LOCUS       DAWBSW010000001        37352 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_223_length_37352_cov_2.655495, whole genome shotgun sequence.
ACCESSION   DAWBSW010000001 DAWBSW010000000
VERSION     DAWBSW010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 37352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 37352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..37352
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223_length_37352_cov_2.655495"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2687..2696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4026..4035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6790..6799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16907..16916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18431..18440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21538..21547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28268..28277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    35796..35805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9932 a         8254 c         8118 g        10968 t
ORIGIN      
        1 cttataccca ttataccgag ccacaaacac ggcgcgctga gttttattgt taaaatcacg
       61 gtcgctcatc tcgtcataga tatgcaatat ctcaaagtcg gcatcggcga gccatttttt
      121 gtagttatct attggataag ctttttccga aaattcctcc gtgctgcgat aataggctcc
      181 gtcctcatat tcaaaaatat caagcgaaat atctacgctg tcgtccttgt tgagactgtt
      241 ctgccaaacg cagtaaacat catcgcagtc atagacaaag gtattattcg caagaatctc
      301 tctgtgctta tatattgtat tcacgtcaaa aataaatata ccgttatgct ccataaaaag
      361 tccgactttt tgaatagttt cggcaacatc ctcggcgcag tcaaggtgat taagactgtc
      421 cagcatacag accgcaccgc tgatcgttcc gtaaagatca agctcggtca tatcctgaca
      481 aagaaacaga acatcaacat tttccgaata ctttttattc tgagcctgag aaagcatggc
      541 ataagccgaa tccgtaccta tcatatcata tccgcgcttt gcaagctcaa ttgtcaggct
      601 tcctgttccg caggcaaggt caagaatcgg ttccttaccc ttaaccccgt aacgttcaaa
      661 aagcgcactg atataatcgg cacgcttttc gtattcgata ttatccgtca gcttgtcata
      721 aacgtttgca aacgcattat agccgctcat tctttttctc cgttcattcg gtcaaataca
      781 aggtcatatc cgccgcagcc gtactggagc gaacggttaa cacggctgac tgttgccgtg
      841 cttgcacctg tttcatcaac gatatcgctg tagacttttt tctcacgaag catttttgca
      901 acaacgattc gttgcgagat tgatttaagc tcgttgaccg tgcaaagatc ctcaaagaaa
      961 tcatagcatt catcaacgga tttaaggtga agtattgcgt caaagaaaag gtctgtgttt
     1021 ttatctttaa gctttttttc cataaattag catctccgtt taatttaatc agcggtatcc
     1081 tttacacctt tatattttat cacattaaaa cgtaaaagta aagttattat tcaaaattaa
     1141 atgttgacgt gtgactctca acaccgccta taagtgttgg tgtgtacgca attataacaa
     1201 tatcggggtc ggttctgtca atataatcta taacactgcc gtcaaaataa cgtctgtcaa
     1261 gaagcgttgt gctctcaaat gtctgtgaaa gatacggggc aacgcatttg ttatatgaat
     1321 cgccgataat gagaatcttt ttgccgtttt tgcagtcatg gtttgttatt tgcgtcagcg
     1381 ccttattgcc gttgagataa gacgcatatg cagagtagtt atagtaatcg gtgttgttga
     1441 ggtaactctt attgaatatg acattctcaa agcgtccggt gctcggtgcg tcgttattat
     1501 actgaacggt aatatttgtt ttctttttcg gataaattat gcttatatcc tcgggatcgg
     1561 caaaggtcag cgtcacaatt tttccttgtg agccgaagca gtatttttca tacacatcaa
     1621 tgttatagct ttcaagattg cctatatcgc tgtcaagctt atatccaaag tctgaattaa
     1681 gtgtgcttac aatttgtcct gcagcccaaa ctcctgtttc gggtttccag tgatggtcgg
     1741 ttttaaagaa attcgagtac cagtcactgt tcttttgagc cagcgaagct ctcagatccg
     1801 agcagttaac accgctcttt ttaagccttt caacaaggtt gtcggcagtt atgtttgctc
     1861 cgtccgtgac tccttccgga agcataccgt cgccctcttt tccttttgcc ggatattgaa
     1921 aatatacaaa gtcgattcct ttttctttca ttatatcgct gaaccactta acgctctcta
     1981 cctgcgtcgt cgcatcggag aaagcgctgc tttcaaatgt cagatatccg tttttcataa
     2041 caataacgga atcgcttccg ctgacgattt tcattccgac agtctttccg aaaagggcgt
     2101 ttgcctcaac atatttcatt cttccgggca aaagcttcgt agtataataa tctatgtttt
     2161 cctttaatgt gtttactttt tcaagataag ccgaaaaaat gcccgactcg gttttaccgg
     2221 tttcctctgc cgtatttttt gggtttttga ccgtcggctc gaattcataa ttttcactga
     2281 aaggatattt cttgctccaa tcttcttgag cagaactgtc attcgatgag cttgagcttt
     2341 cggctcctgt caagccgtaa tcaaaataac gtttcgcacg gtaatatctg tagcaaacag
     2401 agccgaaaaa gaacgcgatt atacaaatga aaactattcc gataataaat ttagctttaa
     2461 actttttcat ttgatatcgc attcctttcg tcagaaatta aagtaaataa acgggttgta
     2521 cgttcctttg ataatatatg ttacggaaat cagaaacagt gcaacaagta caattcccga
     2581 aacgaccgtg taaacaggct tgtttgtatt tattttattt ttcagagttc ggattatcgg
     2641 gaagcagcag ataattccga gcacaaggaa aaagctgtag tttgaannnn nnnnnngaaa
     2701 tataaaacgg agctgtgctg tcataaagcg gcagtgagcc gaagccgaac atatttttaa
     2761 gatattccca tgccgaagca atatccttgg ccctgaaaat gacccaaccg attatcacaa
     2821 ggagcattgt gtaaacatgg ctgaaagcgc cgagcttttt tgtgaagtcc gtgagctttt
     2881 cgatagtgag aatcacaaaa tagaacaaac cccaaatgac aaatgtccag tttgctccgt
     2941 gccagattcc cgtaagagtc caaacaacaa agaggttaaa aatcagtctc gattttttct
     3001 ttactctgct gcctccgagc ggaaaataaa cataatccct gaaccatgtt ccgagcgaga
     3061 tatgccatct gcgccaaaac tcggaaacgg attttgatac atacggataa tcgaaatttt
     3121 caagaaattc aaagccgaac atctttccga gtccgatcgc catctcgctg tagcccgaga
     3181 agtcaaaaag aatttgcaag gtataagcga gtgcaccgag ccacgctgat gagaccgagc
     3241 tgacctcgct gtcaaaaatt ccgtcggcaa cgattgcggc attatttgca ataagcacct
     3301 tttttacaaa tcctatcata aaacggtaaa cgccgtcggt gaattgatcg aaattctcat
     3361 gccgttcctt tatttgatcg gcaatggttt gataacgtac tatcggtccc gcaatgagct
     3421 ggggaaagaa ggaaatatac agcgcaacgt tgcacggatt tttctgtaca tcgccgttgc
     3481 cgcggtaaac atcaataaca taagacattg cctgaaacgt aaaaaacgaa atacctatgg
     3541 gcaaggttat gtttagcgta tcaaggcttg tatgaaaaag cgaattgata ttttcaagcg
     3601 taaatgtcag atacttaaat acaaagagca ttcctatatt aacaataacc gatactgtta
     3661 aagctgctct gccttttttg ttgccgcggt ttttatcgac cgccagcccg agcgcccagt
     3721 taacgagaat tgaacctaac atgagaagaa cgtaaacagg ttcaccccac gcataaaaga
     3781 aaatgctcga aacaatcagc acggcatttt tgaaaggcaa cgacttgaaa attgggttat
     3841 agtatgcaat taaaactatc ggcagaaaca aaaataaaaa tgttgttgac gaaaaaacca
     3901 tactataccc cctttttaaa ataaatttat ttttataagt tttttagaaa taaagctgca
     3961 gcaaacttct ttataaattt ttctcagtca cagacaagcc gtgacagctc ccaaaatgtg
     4021 gagccnnnnn nnnnnaaaca aatttcattt atctttcggc aaaagtctcg atcaaagaaa
     4081 ctgcggcaaa tgcttcaaat acgaaagcgc attgataatc aattatacaa tataataata
     4141 attttttcaa ctgtaaaaat agataatacc tgtttctcgg atttgttgac aaataaaaaa
     4201 gggtatagta taatttgaag gtaatcaact gttgtgaaaa attattgatt acatctaaaa
     4261 attacgaatt gaggtaatta cattatgaat atagcagtgg ttcttgcagg cggctcggga
     4321 agccgaatgg gtatggtcga caggccgaag cagtttattg acatctacgg taagccggtt
     4381 attatccaca ctcttgaagc attcgaaatc aacgagaaaa ttgacgcaat ctgcgttgtt
     4441 tgcgtaaagg catggcaaga ggatctaact gtgtggctca aggaatatga tattcgcaag
     4501 gttcgctgga ttgccgatgc aggagcctca cgtcaggagt cctcgctcaa tgcgttaaat
     4561 gcgattcagg cagagtgcag cggcgacgat tatgttataa ttcacgatgc cgcaagaccg
     4621 cttgtttctc agaaaattat caatgagaac attgaaaagg tcaaggaatt cggtgcttgc
     4681 gataccgtta ttccggctca cgatacgatc ataaagagcg tagacgaaaa aacacttgat
     4741 tcaattccgc tcagaaaaga gctttacctc ggtcagaccc ctcagtcttt caagtattcg
     4801 atcgtaagaa aggcttatga cgactatttt gcaatccccg aggaaaagcg ccccgtcatg
     4861 accgatgact gcggacttgt tctttcggca ggcattaaga taggtatggc aatgggagac
     4921 aagctcaaca tgaaaataac aacaatggag gatctccttc ttgttaaatc cattgtaaga
     4981 gccggaagat aaggcagaaa ggattggtta attttgttcg ctaagagtta caaaattaca
     5041 gagccgaaaa gatttgaaat attcgttgag gatattagaa acgatcatgt tcttgtgcga
     5101 cccgagtatc ttgccgtatg caaggcggat atccgttact atctcggcaa cagaagcctt
     5161 cgcatactta aaagaaagtt tccgatgagc cttattcatg aggcaacggg tgttgttgtt
     5221 cgtgataaaa gcggtaaatt caagagcggt cagagaatcg ttcttcttcc gtgccgtaaa
     5281 tcggactgcg acggaacaaa gtgtgaggtc tgtgtaagaa acaatccgcg cctgcttgac
     5341 aattactgtc cggaatccaa attctgctcc agcaactatg acggcttttc aaaggagctc
     5401 attgaccttg aacatgaata catgatcccg atccccgatg agatcggacc ggaagccgtt
     5461 ttctccgagc ttatttccgt gggctgctgt gctatgagac gtgcaggctt tgccgagggc
     5521 gaaagcgtta agagcgtaac cgtttggggc gacggaataa tgggatatat tatctctctc
     5581 gttgcgaaat acggtttcgg ctgtgaggtc aatatcgtgg gtctcaacaa ggaaaagctt
     5641 gatatgtttg attttgcaaa tgtctattat tcaaacgata tcgacgccct tccgagaatg
     5701 tctgttgcaa ttgaggctgt cggcggcaac gcatccggca tagccgcaaa ccaggcaatt
     5761 gataagctct attcgggagg aaagcttatt ctttcgggcg ttgcaaacga aaatgttccg
     5821 ctcaacacaa gaatggtgct tgaacacgga atttcgataa gaggaacaac aaggagtgtg
     5881 cgctgtgact ttgaaaaagc agtcgaactc ctcacaatac ctgagttccg tgaacatatt
     5941 ctcaggctca tgctttcagt aaacgatgta acaaacatca gagattatta cgcgatattt
     6001 gaaaaggagt caaagtcaac ggctcttgga aaaaacatca tgaaaatttc attttaatca
     6061 agcagcaaag tctgacgcaa gcttctgttt gcgtcagact tttgtgagct tttccgttgc
     6121 aaggcgtttt atttttgtaa agataataaa ttgttccata taaacaaact atccgcagac
     6181 tttgattttg tcaatgtctg cggatttttg gtgattacaa taattattta cttgtatgct
     6241 aaatagatgt acgatgtgcc tataccgttt aagtcacttg tcacgttgcc tgaggtgctg
     6301 ttggaaacct tgaagccggt tgaggtaagc gagagaccct gggaggaaag ggattcgttt
     6361 gcaaccgcca tatagctctt gcatgtgctc tttgtgaagt caaccgatac ggcaggcttg
     6421 cccgtcgcca ttagaatcac aagctgcggc ttgaagccga cggaaagcga acgtgttgac
     6481 gagccgtttc caatataaaa tcccgcttca aacggagcgt tccattttat tttgtcctcg
     6541 gcgtttacat gaatttcgct atttctgata tggctgccgc aaagaagctc tttgtcaaga
     6601 aagcttgcaa agttttcatc tgtgcttgcg gcagggcaga agtcggcatt catcaaaaac
     6661 cagccgttca tcacgagagc atcggtgtct ctgtcggagg ttatatcctc acatccggat
     6721 ttttcgatgt tcaggtttgt cctgaaagtc aatgcatctg caattcttgt aaatatatcg
     6781 tggcatgccn nnnnnnnnnc cttttgagaa aggaacgcaa atgctcagat ttgctttaat
     6841 cattgccgtt cggcagtatt cctgtttttc aagcacaccg tcgtcgttgg caacaaattt
     6901 gtcagtgatt tcgatattgt caattccgac ggcaacaatt gcttttctca gcggaacagc
     6961 cttgtttatc ggaggatatt caacaaagaa ggtcagatcg ttcatatcct cctgtttacc
     7021 gagccattcg acaattctta ttgggagtga aacaatactg tccattattc aacctccaca
     7081 atttctctga ttattgccca gacgtaaatc acttcgttct tgaaatacac cttttcacaa
     7141 cggtcaacgg tgtatttctt tccgttggat tcaacgtgac agctttcctt ttcgggatcg
     7201 tggtcggggg gaccgatata aagataatgt ccctggctgt taaagccgat aacagtattg
     7261 acgccgttaa ggtacatctt gtttttatat ctcagcggtt gaataaaagc tccgaagatc
     7321 gggctgctcc agccgtcgtc ggttttgaga gtgacctcct gaccgtattt ttcaaacatt
     7381 gatttgagag tcatgttaca tcaccttgat ttgattaaaa gcgaagctgt cgtccttgca
     7441 aagcggaatt atatccgccg attttttatc gtaaaagtcc tttgcacgtt caagaaaatc
     7501 ctcgaccgtc ttgctgtcct gctctatgct cacatcgccc gccttaaagc ctgtaatgct
     7561 cggctcgctc tcaagagagc tttttttgag cgcaagtttg tagtacgcaa gccctgcggc
     7621 tgcggcaatg acacgaaaat cttccctgtc ggcatcgggc ttcagccttg agtctatctc
     7681 tttgatgcag gagcggcata aaggaagaat ttcttcttcc ttatatgccg aaatgtccgt
     7741 aagctcgccg agatagtcct ttacttccca gcagctcatt ctgagcctac cgtaccctga
     7801 ttctcagagc ctgttgtgat ctcggcagga gcgagagtct taaccgcacc ggggagaatc
     7861 tttgcaaagc ctgtgataga ggtgatagca atctgctcaa gctgacggtc aacaagctta
     7921 ccgtagtctg tttcaacatc gccgaccttg accatttcaa gagcacatgt cttgtcaaga
     7981 ccgatcatca tgccgctttt tgtaacatga gaggacttga tgaggtttgc gccgaacgga
     8041 gtgataagct tgcctgtgcc atgaaaatta agtcctgcgg cagcatctct gaattccgac
     8101 attttgagta cagttgccgt tacgtcgggc gatgcgagaa tcgtgttaag ctcatacggt
     8161 gcaaactgat tccaaaaatc aataagatcg tcatatgtat attcgtctgc tgcaagtgca
     8221 catacgccgg cagggttttt acctccgtcg ccattgataa gaacgtcaac ggcatctgcc
     8281 ataaggctct gcgcaaggta tgagccgatt ctcttgagcg caattgaaac aacatcaaga
     8341 cgctggaaac gaagcacatc gtaagaggtt gtaatcattc tgcctctttt cttgagacgg
     8401 atggttctgt cgcttacttt gaagtcggct gtttcaagag tatcaccctc cttgaaaacc
     8461 ttcatctcgg cgtttgccga tgtcatttca agattcattg cgcggtaatc gagagagtcg
     8521 atattggtcg ttgcagctat gatatcggga agaaagcttg cctcatccat gccgtagcga
     8581 acggctctgg aaacgtactc cgggaagaga actgcggcat cgcttgtctg gaagaacttg
     8641 ccgacaaccg agctgtcgga gccctgcggc ttgatgccga aacgcttgag ctgacgctga
     8701 taagcgtcga gaccttcaag gtcagttcct ctgtaattct ccgagggatc aagtccctcc
     8761 aatacctgtg taaagctgcg gcctgtgccg tacatacctt tctcaagtct taaattatca
     8821 aaacccattt tggttcctcc ttaaaattta aattcattgt tcgtgtttgt attcttttct
     8881 ttttttgcct tcaactgagg cgtgagaggg ataacgtcct cagccttttt tctgaaagcg
     8941 tctcttattc tgattagttc atccgtttca acacccgaac acattttctc aatactttcg
     9001 cccgaaaggg atggcacgga aagaacagca aattttgctg tctcctcggt aagagagcgg
     9061 cggtaaataa tgccgtcctg cgcctgtttt tcaagctttt taatgtattc ggaaagctgc
     9121 tttgcttcgg attcgctgag cttgacggcg ttgccgtcct ttattgcctt gatacagttt
     9181 tccattgttt caatctcctt tttgtagctt tttgtcactc ctgcattaac ctgagccgga
     9241 accgctacga aagaccattc gtaagcatcg tttatatcgg aaagtctgca atagcagagc
     9301 ttaccgtcat agatttcgcc cttgacgtga ttgcatgggt catatctcat gtcactgccg
     9361 caaacagagc aggtgaacga gccgacggca caactgacac tgacttcctt tttgatgcct
     9421 gcgttaatgt cgccgatgag atctgcgttt ttctcggtca ggggcatgta tgctcttgcc
     9481 ttgacgcatc tgtaatcctc gcctgttacg gtttttttgc ccggaatcga aacacattcg
     9541 gcgtgataga ttcttgcact ctggttcgag ctcttgggtt cgtggtcgaa aattcccgtt
     9601 acaccgacaa acagctctgc caactgcatg agagcctcgt ttgtaaagct ttctccgtca
     9661 cggtcaacgt cgttgtcaca gagaattacg ttgaagctgt aaacctcatc ggcagaaagt
     9721 gcctttacgg taaagcggtt gattttttca agatcctcgg ctgtaacttc gcagggagaa
     9781 acgcttgatt taaatgcctt attcaactga tttcaactcc tgttcaatcc tttcactctg
     9841 tgcacggtaa agccgtgcct ttgccagttc aacctcatct tgaagggtaa tgtcatccca
     9901 ttcaacggaa aatccgtcat ttcggccgtt ggtaatcatc caaaaacggc aaattctttc
     9961 gattaccggt gtcagcagcc tgcgatatgc ttcaagctcg cttgtcagca cgtctgcctg
    10021 ctgagaggac attctctcgg tagacgacca gctcagactc agcatgaacg gaggaagacc
    10081 cgtttttgca acgatctgtt caagcatctg acggacggga acctcgctgt cgaggatctg
    10141 attgtccgct ccgattgctt tgatgctgac atcgcctacg gcaacgaagt ctctgacctg
    10201 gttgccgggc tgcattgcct cactccattc acgggcgacc tgctgtgctc tgtcctttgc
    10261 atatgcacgg tcaacaacat cgttctgcgg cttatatgtg acggcaaacc tgacgttgcc
    10321 gaccctgtcc cagttgattc cgatagtgtt ataaatcttc atcagaatgt tgctgacaaa
    10381 cggcagaccc tttaaaagtg agctgccggt aagctgacct gcttcgggat tgagaaccga
    10441 gagaaggaca agctgaggat aagtgaccgg cctggcttcg gcaagttctc gaacgcatac
    10501 tatgcttgag aagccgtcgt cgccgcgttt cagttcaatg tcgtcaatgt cggcattgta
    10561 gagggcgacg aaatctccgt ttgaatcggt gatcatctca ccgaccgccg ttccataggt
    10621 caaaagctgt tcaaaataag ccgacataaa agcatttatg ccctgctgac ccgagccgac
    10681 cctgacctca cgtagaaagc gacgaagctc cttttcacat gtcttgtctt tgcagtcaac
    10741 atgaaaaccg cctgtgagcc tgatgatttt ataaatcgca gcgtccacaa tagggatagc
    10801 ttcacgaagc tcacggtaga gactgttctg cattgatgag aggggagtgt agctgtcaag
    10861 aagactgaaa ggatttttct tgtaggtttg cggtgctgag accgcggttt ttgcagcgga
    10921 taatttcttg aatattccca ctgaaattct cctttctttt tgtgtggagg caaccgccga
    10981 ttcaggaggc ggccgcaaga gtgttaacga gaaacactca taacgaaaaa gtcattttcg
    11041 ggcgcattca gcgccgtagt gacgaagtag cgtagatcgt ccatggcatg gtcattttcc
    11101 ttgcgcggtg cgtcccgaac tgctttatcg tcccacttgt aaagcgaaaa ttcacggagc
    11161 gtgtccttgc attcgggcga aaaatatatc acccgttgct tgagagcatc ggatacctgt
    11221 ctgattccgt caacgacatc attctttgca ggtatcaccg tgaaacgtcc ttttctgcgg
    11281 atgcattgaa taaagcttgc tgccgatggg tcaacgatga ccgattcaat tctgagatta
    11341 tcggcaagct tttcaagctc ctcataatgc tcggcgtctg tcctttgctc gcctattttg
    11401 cgtgaatcat agtagtattc acgtattcgg taccatttct tttcatattc gccccataga
    11461 ccgaacgaac acggattgac cgtgccgtaa tcgcaggaga tgaaaaatcg tgtgaaccga
    11521 tcggtttcgg gagggacagt gacatggatt tcctcactga acataggata aacaacgccc
    11581 tgagccgcga cccatttgcc ctcgacaaat cttttgtaga aagcacccga gtaaaggctt
    11641 tcgtagcgcc ggattatctt ttccgtcagc gacggattgt cacgcattgt aaaatgaaga
    11701 tagaggcagt tcttctgctt tgcctttagt atccattcac ggtagaacca gtgcatagga
    11761 tgttccgggt tgcagttgaa ccacagcttt gagccgtcaa gcgaacatct tgcgattgcc
    11821 tgctcaacaa acgaccgcgg cataagagcc acctcatcga gcagaactcc gccgagcgtc
    11881 ataccctgaa tcaaagccgc cgaaccttca tcttttccgc cgaaaaggta aaaacggttt
    11941 actctgtcct tgtaggtcat ttcaatatag ttttgcgacg atttaagctt cattgaaaaa
    12001 ccgagggaag ccaaaattga atttaaagga acaataacat tcctttttaa cgatgctatg
    12061 gttttcccgc atatagcaaa tgacgtgtcg gaaaacgcat aaaatgacca ggcaataaaa
    12121 gaaagagaca tacatactgt ttttcccgaa cgtacagcac cgtcgcaaat gatagcgtcg
    12181 taaaaattct tgtccgaact gtcacaccac catgtcatcg cttgaagctg cttttcggaa
    12241 aaattcctga atgatgaaag tgagctactc aagcttatca ttcctcactt ttgcggcgct
    12301 gttttcaagc gccttgtaga aggggagagc actgtcctca tcaccgccgg aacaaagctg
    12361 ctccaagtgc tcaagtgctt tgagacggtc aaaaaatttg atctcgagac cgccgccctt
    12421 aggacgtttg atttcgctga cgttgaaaag gtcaagcttt tccgttccaa gcataggctt
    12481 ttcgtcggaa tatgccagtc tgacggcatc cgaaatattt ccgaatgcaa gctggcgata
    12541 tcctgcgatg atctcgtcac ggttgatgtt tcgcagtttg tcaagccgct cgatttcctt
    12601 tttgatgtcc ttgcgctcaa gcattgacgg gccgaccttg cgggctttga ttccgtagcc
    12661 tgccttggct gcagaggctc gtgcatccct tgtcagagaa aagaataaac aaaacagctt
    12721 ttctttttct tcaagtgcca taaaaacttc ctttcatttt tgtacggtta accgttttgt
    12781 gagagttgcc tctcaatcta agcgcaaaaa acagcaatcc ataacctttt tcggttattg
    12841 actgctgtta ataattattt tatttacaat ttattcacat ttagcgtaag tggatgcatt
    12901 gatttcgatt tgatttgtaa aatctgcatc aagtgaaatt tcggtgcaag gagttgttga
    12961 caaaaaaatc acggacggcc gaagccgtcc gctttaattt gccttattca ttgctttgtc
    13021 tatttcgccg tcaagaataa acgggagcac ctcggttgcc tttgttatgg cggccttgag
    13081 ctgttccgta tcgtctttgc cgaattttga aagaacccag tcggcgagat cgtattcggg
    13141 gttcggtttt gcacctacgc cgattttaat tctcgggaat tggtcggaat tgagatgata
    13201 aataatactt ttgattccgt tgtgtccgcc gtccgtaccc ttgcgcctta ttctcagctt
    13261 gccgcagggg agggaaatat cgtcgaaaat aacgattatc ttctccggcg gtatcttata
    13321 gaacttcgca atgtctctga cagccgtgcc gctgttgttc ataaacgtct gcggcttgac
    13381 aagcagacat ctgtgattgt tgataacaac gtctgcgaca agcgcattgt ttttaagatt
    13441 gttaatctct gtgtcaagct cgcttgcaat atggtcaagc gtcagaaagc cagcgttgtg
    13501 gcgagtgaat tcataatttt tacccggatt gccgagaccg actataagaa attcaatcgg
    13561 tcccggggca attcctttgc ttcgtttaaa gaacatcttg tacctcagga aattgccgta
    13621 ttgaagcttt gacctcaata cgacaaattt gtttcttttc tttttgcggt taaccgaatt
    13681 taacggtcat tgttttcttg gttaccgagt cccagttgtc aatatcggta atgctgtcat
    13741 cgacgagggt gtagacaagc ttgaccttca tgtcgttgag ctctttgatt attgtttcct
    13801 cgtcgggata tttatcaacg tttacaaggc ccgaaatata aaccggtaca cccgaaccgg
    13861 atttgattcc gtattcgcag tcgagatttt tcaaaacgac aagattgttg tttgaatttg
    13921 tcaattcaag gaacgatgtg ataagtctct gcgagcttgt gttagcgata taagccgtgt
    13981 atccgaactc gacccatttt tctgcattct tgacgatgtc ctcagccttg ctcttgtcaa
    14041 ctccgttgtc aaccatggtg ttgacaagct ttactttttg agcatcgttt ttaatggtct
    14101 cgctgagtct ggtgctcatc gtcaggtcgt taatgatagg actgtcaacc ttgacgactc
    14161 tcgtcgcatc ttcgctcttg ccttcggcat tgaccgatgt gtacttgaca acggcggtac
    14221 ttgccttttc gtcaacgctc ggctgaaccg gagcaccttt tccgtcaatg acctcttgaa
    14281 cgcttgcctt ggttgttccg ttagaagcgt ctgcaccgga tataacgtcc gcagtgtcgc
    14341 ccttctttcc gcagccgccg acggcgagca caacgctcgc tgccatgaca agcgaaataa
    14401 ttctcattaa aattttgttg ttcataatct acctcttact tctgccatct gtatggcttc
    14461 tttttcttga cccaatcctt cttgacggat tccttggctc ttgcaattgc aagagcattg
    14521 tcgggaacgt catcggtgat gactgagcct gctgccgtgt atgcgttgtc tccgacctga
    14581 acgggagcga caaggcaagt gtcgcagccg atgaacgcat tgtttccgac ttttgttctc
    14641 gccttggatt tgccgtcata gttggcggtc gcacagccgc aaccgaagtt tacaccgctg
    14701 ccaacgtcgg aatcgcctat atatgtcagg tgagcgacgc tcgtatggtc gccgatgacg
    14761 gaattcttga attcaacaaa gttaccgacc ttgactccct cgccgataac cgaaccggga
    14821 cgtacacgaa cgaacggacc catgtgtgca ccgttcttta tctccgcact gtagcattga
    14881 acattgttga gcgaaacgtt gtcaccgata acgctgtcct caatgagact gttcgggcct
    14941 atatcgcagt ctgcaccgat aacggacttg cccttgatta tcgtgccggg gagaatctcc
    15001 gtatcagctt tgattttagc gtcgggagag atgataactc cgtccgcaca cggaatcgaa
    15061 acaccgttga gcatatgctt tttgagaatg ttcctgcgag cgatatcgtt gagcagctgg
    15121 agctgtaccc tgtcgtttgc gccgagaacg atatcgggtg agcttgcatc gtaagcgacg
    15181 gcattgagac ctttttcgag aatgacctca actgcgtcgg taagatgata ttcgatcgcc
    15241 tcatcattct ttctgtcgtg cttttcgatt ataccctcga gcgattcgag aagaaccgag
    15301 ccggaaaacc aatatgcacc cgagtttacc tcattgacta tcttttcggc ctgtgtagct
    15361 tccttttcct caactatcct tttgagctta ccgtctcttg aacggattat tctgccgtaa
    15421 ccgaacggat cggcaacctt tgcggagata accgttgccg cattgttacc tgccttatga
    15481 aaatcaagag ccgctttgag cgtttcggcg gggataaggg cagcgtcacc gttgagaacg
    15541 agcacgttgc ccgggatatg ctccttgata aaatcctttg cctgcatgat tgcgtgaccc
    15601 gtaccgagaa gctccttctg ctcgacggtc ttgaatctgc cgccgaagtg ctcgtcgatc
    15661 atctcacgct tgaagcccgt aaccaggcaa atatcctcga cgccggcatt gaccgccgcg
    15721 tcggtgaccc aatctatcat aggtttgaac aaaacctcgg cgagaacctt aggctttgac
    15781 gatttcattc gcgtgccttg acctgctgcc agaataactg cacaatttga actcattttg
    15841 cttcctcctc agtaaataaa aatctacata ctaattattg cagaaaatca aaagaaatac
    15901 aatagaattt ttcatttttt ttacgacaat tttcacaaaa gtttaggcgt ttatttgtgc
    15961 ttatttattt caaaaatgta tagcaatagt ttgtcccgtg tgttataata atacgaaacg
    16021 tgttattggg attattctgc ccaatcgctg ttaacaattt aggtaataat tttaaatgga
    16081 ggtaataccc atggcaacta agtatactta ccttttttcc gaaggtaaca aagacatgcg
    16141 taatctcctc ggcggtaaag gtgcaaacct tgcagagatg accaacatcg gtcttcctgt
    16201 tccccaggga tttacgatca caacagaagc ctgtacaaag tattatgaag acggcaagaa
    16261 gatcaatgat gagatcaagg gccagatcat ggagtacatc gagaagatgg aagagatcac
    16321 cggcaaaaag ttcggcgata agaagaatcc tcttctcgtt tccgttcgtt cgggtgcccg
    16381 tgcttccatg cccggcatga tggacacaat tcttaacctc ggtcttaacg aggatgttgt
    16441 tgagacaatc gcagagatgt caggcaatcc gcgttgggct tgggactgct acagaagatt
    16501 tatccagatg tactccgacg ttgttatgga agtcggcaag aagtacttcg aggagcttat
    16561 tgataagatg aaagccgaga agggcgttac tcaggatgtt gagcttacag ccgatgacct
    16621 caaggttctc gcaggtcagt tcaaggatga gtacaagtca aagatcggtg ctgacttccc
    16681 gtcagacccg aaggagcagc tcatgggtgc tgttgaggct gttttccgtt catgggacaa
    16741 cccgcgtgct aacgtttacc gtcgtgacaa cgatatcccg tattcatggg gtactgccgt
    16801 taacgttcag tcaatggcat tcggtaacat gggtgacgat tgcggtaccg gtgttgcttt
    16861 tacccgtgac ccggctacag gcgcaaaggg cctcttcggt gagttcnnnn nnnnnnaatg
    16921 cacagggtga ggacgttgtt gctggcgttc gtactccgat gaagatttcc gagatggctg
    16981 acaagttccc cgaggctttc aagcagttcc aggatgtttg ccatactctt gaggatcatt
    17041 acagagatat gcaggacatg gagttcactg ttgagcacgg taagctcttc atgcttcaga
    17101 caagaaacgg taagagaaca gctcaggctg ccctcaagat cgcttgtgac ctcgttgacg
    17161 agggcatgat cgacgagaag caggctgtta agatgatcga cccgagaaat cttgacactc
    17221 ttcttcaccc ccagttcgat gctaaggctc ttaaggctgc tacaccgatg ggcaagggcc
    17281 tcggcgcatc tccgggtgct gcttgcggca agatcgtatt ctcggcagag gacgctgtag
    17341 agtgggctgc aagaggcgag aaggttgttc ttgttcgtct tgagacttct ccggaagata
    17401 tcacaggtat gaagtcggct cagggtattc ttacagttcg tggcggtatg acatctcacg
    17461 cagccgttgt tgcccgtggt atgggtactt gctgtgtatc gggctgcggc gacatcaaga
    17521 tggatgaagc taacaagaag ttcgagcttg caggcaagac cttcgttgag ggtgactaca
    17581 tctcaatcga cggttcaaca ggtaacatct atgacggcat cgtcccgact gttgacgcta
    17641 agatcgcagg cgagttcggc agagttatgg agtgggctga taagtacaga agacttaagg
    17701 ttcgtacaaa tgcagatact ccgacagatg ctaagaaggc aagagagctc ggcgcagagg
    17761 gtatcggcct ttgccgtaca gagcatatgt tcttcgaggc agacagaatc gctgctttcc
    17821 gtgagatgat ttgtgcagat actctcgagg agagagaggc tgctcttgat aagattcttc
    17881 cgtatcagca gggcgacttt gagaagctct atgaggctct tgaaggcaac ccggttacaa
    17941 tccgtttcct tgatccgccg cttcatgagt tcgttccgac tgaggaagct gacatcaagg
    18001 ctcttgcaga tgcacagggc aagtccgttg aggacatcaa ggctctcatc agctctctcc
    18061 atgagttcaa cccgatgatg ggtcaccgtg gatgccgtct tacagtcaca tatcctgaga
    18121 ttgctaagat gcagacaaag gctgttatcc gtgcagctat caacgttaag aaggctcatc
    18181 cggattgggc actcgttccc gagatcatga ttcctcttac cggtgaggtt aaggagctta
    18241 agttcgttaa ggacgtagtt gttgcaacag ctgacgctga gatcgcagca gcaggcatcg
    18301 acctcaagta cgaggttggt acaatgatcg agattcctcg tgccgctctt acggctgacg
    18361 agattgctaa ggaagctgac ttcttctgct tcggtaccaa tgaccttact cagatgacat
    18421 tcggcttcag nnnnnnnnnn gcggtatctg cggcgagcac gacggagatc cttcttcggt
    18481 agcgttctgc cacaagatcg gtctcgacta tgtatcttgc tcaccgttcc gtgttccaat
    18541 cgctcgtctt gcagctgcac aggccgctct cgctgactaa gcttgaacac agtcttataa
    18601 accttgtttt cggacaagca taaatgatac aaagcccccg gagcgtatat gcttcggggg
    18661 ctgttgtgta ttccgtttta tgaggcagac aataatgttt ctcgtctgtc cattagttca
    18721 ttcagttttg ctgtattatt tctgaaaatc aaagctttaa gtttatatat attgtttttt
    18781 gaataatctt tattaaaact ccgttttctg aattatgatt tattttgacg gaattatcac
    18841 ttcaattacg acttttacat tgttgccaac gctgagcgaa aaagaggaca ttcaaaaata
    18901 aataaaaata ttataaaata ataataaata atattgacaa ttacgaatta tactgtataa
    18961 taaatacata attatgtgaa agagatgata aaatgaaaaa gcagttaatt caggttattt
    19021 ctgtcattct tgttattctt acggttcttt cggtaccgac gtttgccgga ttttcggatt
    19081 ccggcatcaa cggtgtgata acggagataa ccgagctttt gaccggaaca acgtccgccg
    19141 acgaggaaac aacggacgaa accacaacgg agcccacgac ggatgagccg actacggaac
    19201 ctactaccga accaactaca gaaccaacta ccgagccgtc aaagacattt gacgactaca
    19261 aggacaacga taaggtggcc gtcatgtata tctgcactca gcaggtcgga ctcggccatg
    19321 cgtggatcta cattgagaac acggcggaat gcgatttgaa ggtcggatgc tatgatttga
    19381 agcctgattg cggcgtctca atgggaacgt ttttgctcag ccgttcggac ggcggcggac
    19441 tttattataa cgtcgaagcc tattgtgcaa ataagtgggg acttaaaaat aagagttggc
    19501 tcaaaaccga gcttacaaag aagcagctta ttaaggtgag cgatagaata aaacagtgga
    19561 attattggga tttttatttt aactgcactt tttttgcagc cgaaatttgg aactgtgctt
    19621 caaagaaaaa gataattccg cttatgtttc cgttctttat aaagtggcag attctggcga
    19681 agggcggaaa caaagatgtt gaaatgaagc ctgtcgaaaa aacagattgc ttcaaacagc
    19741 gtgggtcagg aaagaatgca cacatcgttc aggttaagga aggcacactt gattcaaaat
    19801 tgttttaata ataatcggaa tccgttcata tgggcggatt cttttttgta ttgaaaaaga
    19861 aaaaaaatat ggtataatat aaagaaaagg agctaatttc gatgaaagca ttattatgca
    19921 aacttctttc gggtgtaatt acgctgattt cactgatagc ctttgcagtc aatcagccga
    19981 caattccaaa tccgacaaaa aaggttgata tgtcaaaatt tacgctcacc tttgaggatg
    20041 aatttgagga cgagcttgac cgcagtgttt ggtcgggaca ctatacctat ggtgacaaat
    20101 catcaattcg caaaggttca tattggaaca attatatggc ttataccgag aacgggaatc
    20161 tcattattcc gcttagatat ctccctgacg gcatgggcgg cacgggtgca ggctggtaca
    20221 cggcaggtct cgataccgat tccgatgcgc caaacggctt ttcgcaaaaa tttggctatt
    20281 ttgaatgtcg ctgtattctt cctaagggtg ccgatatctg gagcgcgttc tggatgatga
    20341 atgaaaatgt ttacacggtt gacggctcgg gtcgtgacgg cacagaggtt gatataatgg
    20401 aaagcttcag ctacggaaaa cgtatctcaa atgtcattca gagcaatctt cattgggacg
    20461 gctatgacga agctcatcag accggcaaag cgaataaggc atatgtcatc ggtaacaatc
    20521 cgtatgagga gttcaataca tatggcttgg aatggaacga aaacgagtat attttctatg
    20581 tcaacggcaa ggaatactgt cgctcaaatg ccggcggggt atgtcaaaat ccgctctacc
    20641 ttattctttc ggttgagatg tggggcgaga acgcagttcc cttggacaga gatgccgaca
    20701 gcgctccggc ggattatatt gtagactatg taagagctta tcagtacaat gatttgctga
    20761 acaaataagc tttaaatata aaaacaacct cctaaggtgc aaaccttggg aggttgtcga
    20821 tttattctaa aagacaaaat cgctttgcca gatatcaacg tcaaagtctt ggaaaacatc
    20881 cttgagacgc ttatgaatat cgtcctttga aattcctttt ttcagtatta cttgcaagaa
    20941 tccgccgccg cctgcgccgc atatcatctt tgcatcggtg aggtcatcta cggtcatgat
    21001 tatttgatca ataagagtgt tagtgctgcc gccgtcaatc attttggaca gcccccaatg
    21061 agcatttaaa aggcttgcaa attcgtctat atcgcctcgc tcaagcgcaa aacgcatgag
    21121 agccgcaact ttttgaatat tgcggtgagc ttcaaggctt ttcggctcat tgccaacgta
    21181 gctgccgaca acatcacgca acagatttct tgccaaacgt cgctgacctg tataaatcac
    21241 agcaaagcgc tcggaaagct ctttttttgt gctctcgctc agaattacat gatcaacgtt
    21301 gattttttgc tcgatccctg aatcggaggt tatgaattta attcccgggg ttatgccgcc
    21361 gacctgatcc tgccagccgc cgcccgtgga catgatctgc tccattgcga ggacggttga
    21421 ataaagcttg tcgtcatcat attcaatgtg catgaactcg aaaactgctt tgaccgctgc
    21481 agccgaaaga atcgaagacg taccgagtcc cgagcctttg ggaacattcg taacttcnnn
    21541 nnnnnnncgc cgagacggct gagaacatcg tcaagactgc cgcccgattt cggaattatt
    21601 ccgcaggcaa gcaggcacgc cttttgaagc gcaaacgggt caaacggatc ccccgtattc
    21661 tgtaagggtt ctatgttgtc aaattcgccg tgaacgtcca tatcgcggct gtcaaataca
    21721 attctgtgtt cggaaatctt aacaagtctg acctcaacag gcagttcgcc gttgagcgag
    21781 attgcggcat ttagaactac gccgccgtgc tcaaggcaat gcgggcaggt gtctgtccag
    21841 cctccgcccc aattgacacg aagcggaagc ttgactaccg tttcgttttc ttcaatttta
    21901 cattctgagt taaaacggag ctccttcatt gtgctgctca gaactgtatc cgcaatcagc
    21961 ttaaagcatt tggcaatgta tttttcatca ttgaataccg ttccgaggta gtagtagagt
    22021 cttatggcat atgaaaaatc cggaagactt gaagtgtcga gcttgttgaa ttcgttttca
    22081 agccactgag actgaatagc ggtgagttta tttgctttga gaattccgaa gcacttactt
    22141 gcaggcttgc cttcaagaat gtagttcttg aattcgtcca ttctgacaag ctcctccata
    22201 cgctttgtcc agtctatcac ggcgtcagga tcggcatcgt tgaagcccga gcagagagat
    22261 ttttttgttg atttcagcca tgctccggta ttttttgact catttacaat tatatcataa
    22321 aggttgagcg ccgcattaac agcctcatta accgtcttac attccggata aagctttgcg
    22381 ttccaaaggg tgtgctcgtc gccgtcatcc cacaaatcgc ttgtgtgcag agaaagtcta
    22441 tcggcaattt cctcaagatt tcgaccgaaa atcatgcctt ttttcggatt gtcatgtata
    22501 tccattattc ggcagacaaa acaaccgtca gtctgcttca agccatggat aagcacatta
    22561 tccggaataa tctcgtcgtg aaggtcgata aagctgatat agcagttctt tcctacaacg
    22621 gctttggaat gaatatatga aacttcaaga taagaaccgt cgccgataac ggcaccctct
    22681 gagcgaacgg aattatatgc tgcaatgtca gggtcggaaa tgctgctgtt gatttgtttt
    22741 ttccagccga gagagctgta ttcctcaaca cccttattca tcagcttcat aatttccggg
    22801 attgagccga aatgaatgaa acgtgcaggg gagaggttga gcagcttcat attatattga
    22861 ccgattgccg accaaagtgc tgtccttgcc gctgtcagct catcgcagaa gctaccctcg
    22921 ggactctgag ttaaaaagtt ttcaaggtcg gattcttcgg caaggcagta ggctatatcg
    22981 ccgtaaaggg aaagccttgc tctgtcatta accatgatat cgtatttctc gtcggtatca
    23041 accgtcccgt aaagcttttc aagaatattc gggctgagcc acaacgctcc cgtgtcgatg
    23101 ctgcacttgt ttctctcgtc aaccgcaccc tctgctttga gaatttcaat ggtctgttta
    23161 tgaagaaatt ttttgacatt gccacgttcg cttttgaggt ataccccgtg atttgttgca
    23221 acttccgagt cctctttaaa gctgataaca gcggcattcg aggatccgaa atcgcacata
    23281 agcggattga aaagcagagt aacatccccg gaaagaagaa gcataccgtt tttcaggcgt
    23341 ccgggaatac ttgccatgga aaccatgaac atatcaaaaa gcgttgccgg caaaccgccg
    23401 aacgaggtcg gaattgggct gaaaagctta ccgagagcgc tgtattgcgg agttctcgag
    23461 ctgttgccgc ctgcatggat aaccataatt cttttgctcg acaaattgcc gtacagtctc
    23521 tttaattcac ggataacgga gagcgtggat ccggccgagc cgacacgctt attttccctg
    23581 tccgaaacaa caagaaaatc ggtataaggc gaaactctgc cgacggagcg tctgtattct
    23641 atttgcttgc ggtatccgtc ggcttgtgcg gtgttgcttg ccgttattac aatgcagtcc
    23701 caatgcggaa aattcggatt gcttaccgag cggtcaaaat ccgcctgcga atccatgaga
    23761 gactgctgca aaaacagcga caccatagcc gtatttgtca gtttatttga aaaaacgtta
    23821 tcggacatag gttacctcct acggttcttt tacgtcttgc tgattcttga tttaagctta
    23881 tgaattctgc cgaagaatag ctctttttcg gctttgtttg cgccaaaggt aagcatgcaa
    23941 acggcgagaa tcacgcagaa tgtaactcct ccggccaaaa gggaaaggaa gctgttatga
    24001 atgaaataag aaacagctgc gcttccgaga caggaaatga tcatgcttgg cagaattcct
    24061 ttaaatgatt gaccgaaaag aaacattacg tcaatgtgaa tggctttttt atagtaaatg
    24121 tttatcagta tgatttgacc gatgattgtg aaaaccgccg ttgccgccgc cgcaccgaac
    24181 atgccccagt attttatcca ccaaattgtc agaagaatat tgccgacagc cgttacgccg
    24241 agacataatg agcggacatg ccttttacgc aggacgtcga gaatgttaac gataattccg
    24301 tttgacatat ttataaacat cggaaccatt atcataatgg ctataatcca tgcgtcgaga
    24361 taatccgtgc cgtaaactat cgaaataaac ggacggccga ctgcaataaa accgaaaaga
    24421 accaatcctc cgacgattac aacaagcctg cagggctgaa caagggtttt tgtgagttct
    24481 gaaccctcct tgccgtttcg catgtccttg gcaacctgcg gcatgtacat tgaaatcggt
    24541 atggtggtca tggctgagaa ggttgaataa atgtacattg caaccgaata taccgatacc
    24601 gcttcggggg acatgacgat acctattacg aatttatcaa cattgttgtt agccatgttg
    24661 acaatcgttt gcagaaacat agcgagagcc agcggaagaa tctgacggaa aattccgacg
    24721 ttaaagtatt taaaagtgaa tttaaccttt aattttatta tacagtagaa aattgtaaaa
    24781 ataaaaatgc aaattgtgag cactgtgtct atcgtgacaa gcactatggc actgtgcttt
    24841 acgatcagga gagaaacaac aataatcgaa cggacaataa gataggtcag ggaaacgatt
    24901 gagccgaggc tgtatctttc aaagccgagg acaacaccgt taagggtttg cacaagaaaa
    24961 ttgaatacaa gcttcacggc agatataata aaaattaact tgcctgtttc tatttgttcg
    25021 ggagtaaggg atttgtgata tattgtacca atcaaaagat agaatatgaa tacaccgata
    25081 catatgagcg ccgaaagaat acaggttgtc gtccaaacgg tggaaatatt ttttctgata
    25141 tcgtcgtttt ccgtaccgtc ctttttacaa agagaaatgt ttcttgtcat tatcgtacct
    25201 gttccgaatt caaacagggt cagataggta agaaacgatg tcatggtctg ataaacgccg
    25261 tagtcggttt tgccgagcat tctaagaata aatgcggaca tgaaaaaacc gatgacggtg
    25321 ttaagaattg tatagatata tgaaattgct attcctttac ctctgcgatt cattttgagt
    25381 ctccgatctt ttgcttaatt cgacggtatt tgccaatcgc tcttgtaatc agtgtgtgac
    25441 gattggctgc atcatgaagt aatgcagata tcgaggcagt gccgttaaaa ttgtcaaaga
    25501 aataatctcg gtttaagttg taaccggttt gaatgtacat tgaactgttt tcacgcaaag
    25561 catcatcttc gcttacttct gtaaattcaa tgtcacaaag gctgttaaat gtgcttatcg
    25621 ccttttcgct gttgataaaa gcaagcgtta tgcctgtgtt gtcattcatt tgcggggtta
    25681 ctttttcgca accccaaaaa tctgccattg tgaagtcgga aatacgttcc agcttgcgga
    25741 aagggcattt aaaacaactt tttcgtatgg ttacactgtg aacaaaagcg ctcatataag
    25801 ggtcatcaac aacggtttga cagtatttat ttccgtttga aaaatcaacg gaaaaagaat
    25861 attttttcca gccggatatt ttatttcgga agctcacctt atcggaatgt gcattgttct
    25921 tttcttcaag ctctgaaata tacgcactcc aaacagcggg ggagggaacg ccgtgacata
    25981 tcatatccac ggttaaaaga ttatcatatt tctttttaag atatgcactc agtccttcaa
    26041 tttgacacgg tgtgccggaa aagaggacag ggcgccccga catcaaatct ttttttattt
    26101 ctctgaaaag atttgcgtta tcttcgcatt gaacatattt tgacccgcgc agctttccga
    26161 tatcctctgc tttttcaatg ccgatgtgtt tcacggattt aaaatccgat gaaaatgccg
    26221 caccgtaaac aattcctccc tcggcaataa cctttgaagc gagaaccgag aaaaatccgc
    26281 ccgaggagct gctcatgcga accgagctgt cggttgttct tactgcatat atttttgaag
    26341 cctttgaacg actgctttcg tttaaaacgg ggcacatcga agcacacgct ccgcactcgg
    26401 tgcaaagcga gaaatcaacc tgtgggtaac gaaagccatc ttcgtcggaa caaagagaga
    26461 ttgcgttacg agcgcatacg gcagagcatg cggcacagcc tgtacagttt ttcggtgctt
    26521 taatcggctt catattttta ccttccgaag tttgaaataa gatccttagc ggcgctttcc
    26581 gccctctttt gatattcggg gattacttcc ttcagtctat tatgaataat ttcctgattt
    26641 tgctgcagcc atttgaattc atcaaatacc tcgtcggctg attttaattc ctggaccgga
    26701 agaacatagt gtttataatc accgaaaagg tcggttgcaa ttccttttga ctttattgag
    26761 tagccgagaa caagagtcgg aacaaggctg gaatatgccg caatagacgc atgggttctt
    26821 gcagcaacca aaagagagca ttttgaaata atgtatttca gttggttaca gctgtaatcc
    26881 tcttttggaa gccgtacacg gtcattgctt tcaaatcgct gttttaaaat attgagaggt
    26941 tcatagtcct gacccttaaa atcgttcata taaacatgag ggataagtat tatgttcata
    27001 tcggtatgag cgagaaggtc ttcaataatt tttatgcatc cgtcaataac aagcatatga
    27061 tccggatttt cttccatcga aatgggactg agatttatcc cgacaacgcc ttttttccac
    27121 cagctttcat ctgtttcaac agcttcgggc tcgagggtaa acgcagggtc ggctgtcaaa
    27181 aagagcttgt ctttgtcaaa accggagtta agcatgatat tatatgtcat ttgttctctg
    27241 atataaagtc tgtcatatga taatagggca ttctttatcc tttcatttct taccgcagac
    27301 tcgtcaatag atgctgccca aagaacaaga gggatttgtt ttattttgca aatgcgaaca
    27361 agagcatcat gttcaagcaa aacgccgtag caatatgtgt cgccgccgat gtgaacggca
    27421 atgttatctt tagcggattt taaaaagtcc ttttgtctga gcatttcgta aggaaccgta
    27481 ttgtgaaaaa ctttattcca tgctgctttg aaaaaaagcg acggtttgct gttcaattca
    27541 cggaatttca gcacttcatc ataatattta aaatcctgat tttctttaaa ctcgttctca
    27601 cttctgactg tcagtgatgt tttaacattc aatttgcgaa acatggctga gactgacttt
    27661 gcaattgcct cagtgcctct gttttcgcga cccgtatagc cgcccatgac gatttttttc
    27721 attcgtgccg ctcctcttta ttttgattga tatatcggtt ttgcaatgct gcacggaccg
    27781 aattcgtata atgattttat atctttaccc tcaagaagga aatttgacag atgaataaaa
    27841 cgctttgctg cttcctgagg agaccaattt tgtgtaattg tgtaatatgc ttctttgccg
    27901 tattttttgc aaagctcggg gttatccgtc atagttcgca gcagagaata catctcatca
    27961 attctgccgt tttgatatac tctgccattt atgccgtctt tcatcataaa cggaaccgcg
    28021 ccgatagcac gatttgtaag cacggcacag ccgctgttca ttgattcatt aagaactgcg
    28081 ccccagcctt cgttatagtc gcttgtgaag aggtaaattg acgatttttc cattaatctg
    28141 cgaacttcgt cctgcgagag cgcacctgta aattttacgc agtcatcaag accgttttta
    28201 cttacttttt ctttcatttt agaaagcaac ggaccgtttc cgaccattgt gagtttgaat
    28261 tgaatccnnn nnnnnnnttt tttcaatctt ctcgccattt ctattgcgtg ttcactgtgc
    28321 tttaattcga taaatcgacc tgcccacatg agactgtttg tctctttctc cgaaagcagt
    28381 ttatcaatat tgtcatattt aattacttcg ggaaaatatc cccatttgaa cattttgtcg
    28441 gggtaggcgt gtattatacg gcaatccccg gcaacgtaac ctccggcaca gagcagatag
    28501 aaattctttt ttgcatattt tgtgtgataa ttgtatgaat ttttaagaac tctcggatca
    28561 aggagtcgga tatatccctg tttgaataat ctttcaagat atcggaatgt gattttatcg
    28621 gtattctcga ggcgattttt tatccactcg gttgatgaac cgccctgtat tacgatatcg
    28681 gcatcgtcga tgagcttttt ggcaagcttc ttttgttcct cgccgtcata ggtgcagacg
    28741 ataaaatcat atttgtggtt catatcctca tagccgaagt ccaggcgttc ttgctctatc
    28801 ggttcggttg caatgaagtg atagttgtcg ccgatcagtt ttttcatttc ccttgaaaag
    28861 ggaagctgat gatgattcat aaaattggag ataaaaacga ctttcatata gctctcctcg
    28921 gataaatttt atatcattat ttttttataa tttctttata tatttcaaca agctgttcgg
    28981 ttgatttgtt tatgtcaaga tacggtctga tgtagtcttt tccgttttgg ctgaaagctt
    29041 ttgccttttc atcatcgtta aaaattctga gaataaaatc ggcaagctga atatattctt
    29101 cgtggcggaa aaggaatccg tttttgccgt gctcaattgt ttcgggaatt ccgccgacat
    29161 agcttgctat gctcggaaca ccgactgcca tagcctctct gagtgtagag ctgtgatttt
    29221 caattgccga aggaacgacc ataacgtgcg attgctccat tctttctgcc atttgctgtt
    29281 gagtcagtct gcccgtgaaa acaacgttgt ctctgatgcc gagcttggtg ataagataca
    29341 taagatattt ttcatatccc tgctgtctga atttggactt gaaatcggtt tttgaaaagg
    29401 gatcctgaaa tcccggtgca taaagcttaa catcaggcac gcttcttttt acgattgcga
    29461 gagcctcaag aaggacgtgc agacctttaa gaggataagc ggaagcacaa gtgaaaatcg
    29521 aatgcttttc gcaagtttca agcgaccaat tctttttaaa aaatatttca tcaatgttaa
    29581 gatgatgaac aaaaagcttg gcattgggat tgatatatct gcaatgagct gttgcccagt
    29641 cattttcaat aataatattt tccgaataat taagcatctg cttttctatt tctgcttttt
    29701 tgccgaaaaa cttttgctcg tcataaagtc cgtcatgctt aataaaattt ctcagtgaat
    29761 aagctttttt tatttgagat ctcgtcatgt tgctcaggta atagtattca atggattcca
    29821 ttacaccttg catttcggta acagagggga tacccttttc ttttgcaatt ctgagtgcgc
    29881 acagtccgtg agcatattcg gtgccccact gcatgattat atccggctta gcttcgtcaa
    29941 ttacctgttc ccactgtttg atatgtgcag tgtcattata ttcatatatt tttttgttat
    30001 cttgagggac aaggtaatat gtaatattgt cttcttcaaa atgttcaact gcattgacgg
    30061 gggcaacggt tgcaacagaa atctctatac cgtcggtttt ttcgaggccg agcaatgtcg
    30121 gctccatcca tgttccgctg atggctttaa cattgaattt gcgctctgcg gctgcgatag
    30181 gtctgttggt tatccataaa actttcattt tataagcggc ctccttttaa tgatgaagtt
    30241 attattaatt aacagctcat gaaagtaagg aacaagaata aacatattca ttgaataaat
    30301 aactatagag aaatagttta aaataccgga tacaatgtaa aaaatactta cagcgataat
    30361 aattttttta cctgcataat cagaatctat aaattgcttg cgtatataat tgaaaattga
    30421 aacataataa acgaaaatca agcaaccgaa tttagcaata taatctatcc aaattgagtg
    30481 accgccgttt ttaaacttat tcggtgtcag ctctccaagg aagaaattgt gtcccattgc
    30541 acggaagctt tcgatatagt gcgtgagtct gccctgtgtg cttgtataat ttttttccaa
    30601 gcctgtgctg tattcattga taatttcagc aaatgaggca tccctgaaga aaatgtcata
    30661 aacatcctgc aagcgctttg taacctctat ggggaaaaaa ctgtttttta atatccagtt
    30721 taatcccgtc ggcaaaagaa ccatgaagaa caggcagaat ataacggaaa aaagaagaat
    30781 ttttttgatg tctacggttt ttacgattaa gatataaagt gttattatta ttgtaagaag
    30841 caacggcagc gacatctgtg ccaaatagag aacataaaga gtcaatgcca tatatgctgt
    30901 tgaaagaatc ttatgctttg aacgtttgaa attgcaaaga acaataaaat aaataactgc
    30961 aaaggtgtat atcgaaggga tatttaccag cttttcgatt cccgtaccgt ttacgctgag
    31021 cacgtcaaaa ccggaatctc tgataatcag cggatttctt aaaagctcat aaatatctac
    31081 aaattctttt agaattacct ccgcaaggat atagagcagg atgattttcc gttccttttc
    31141 tttgtcatgc atatatgcgc cgatacacat catcacgaag cagtaaaaag aaatgagaaa
    31201 gcccgatttt gtgaatgact catgcgaaaa aaacatatac gccagcgaaa gcagcatgaa
    31261 cgatgcaatg ccgtaaacct tcatgtcata gcatagaaaa tttctgcttt tctgcgaggt
    31321 tagaataaag aaaacaagaa ggagactgat tccgaaagct attgttccgt taagaggagg
    31381 aataagcagc caaattccgc cctgatagaa gctgataaat gcacaggata aaagcacaat
    31441 aaacatgtgt gttattttct gacccgcaga aacatttatt tctggcatgg gctatccctc
    31501 ctttcaatag tttatctttt aataattgat ttaaaaatat ctctcttttt gtaaacgcgt
    31561 ttaatctgat tggcaactgt ttttgggatg atctttgccg gagtagagag aaaaaccatg
    31621 ctgaatttat aaaaaggatt gctcattgag cgtttgctga acaatttttt cctcgtcatt
    31681 ttgtcgtaaa aattatcgcc ggttgtttct tcatcaacaa gatatctgag gctttcgtcg
    31741 cagtaaatcg tatagccgta ttttctccaa gcacggatcg tccattcata gtcggagccg
    31801 tagtggggga ggaggagggg atgaaaaccg ccgattgtta taaaatcgct tattctgaag
    31861 aacagacttc ttgtcgaggc acagttgcct tctccggcac cgccgcgctt aactgtcgtt
    31921 gttgcaaaat cgtaatcaac cgcaccgtca aagaccttgc ctgtctgacg gctgactcca
    31981 tagcccgtga gaagagtatg ctctcgggta tcaagaattt taatcgcttt ttcgatatag
    32041 tcatcctcaa attcggaatc gtcatttgca aacatggcaa aggcagatct gtcggcgttt
    32101 ttgacagtcc acttataagc ctcatgcaaa gcgcctcccc accaaaggtt gccgtcacct
    32161 tgaatgataa cggcttcggg gatttttgaa cgcaccatat cagccgtacc gtccgtacag
    32221 ccgtcgtcga cgagcaccag ctttatatcc ttataggtct gtcttaaaag ctgatccaca
    32281 aatttttccg ttattttata tctgtcatgt actgccgtga atacatagat catagttatt
    32341 cctcttaaag tctccagtat ttcattcctg agttttcaag ctcctcgacg gtgtataagc
    32401 ctttaacgtc aaggaaaact ctgctttctg cggcgagctt gtcgttgaac agctttgatg
    32461 ctccgtccaa tccaaggtct ttgaattctt tatgagcgac tgccataata acgcagtctg
    32521 cgtcatggac atcgttcata tccgtaagct cgatgccgta ttcggcaagt gcctccgcct
    32581 tgtttgccca cgggtcaaca acaagcggct tgatctcata ctcatggagt ctttttatga
    32641 tatcctcaac ctttgagttt ctgatatcgg ggcagttttc tttaaaagtg agacccataa
    32701 tgacgacctt actgttcttg ggagcctgtc ctgcttcaac catttttttg atagcggcat
    32761 cggcgacgta tttgcccata ttgtcgttta caatacgtcc gctgagaatg atttgactgt
    32821 gatagccgag cttttccgct tcataggtga aataataggg gtcaacgccg atgcaatgtc
    32881 cgccgacaag acccggtctg aagccgagag cattccattt tgtattcatt ccgtcaatga
    32941 cctcgtttgt gtcgatgttc atgcggtcaa ataccattgc aagctcattc ataaaggcaa
    33001 tgttgatatc acgctggctg ttttcgacga cctttaccgc ctcagcggtc tttatgtttg
    33061 atacgggatg agttccgacc tcaataacga tatcgtaaat atttttgatt tcgtcaagtg
    33121 actctttatc cattccagaa actatcttat gtatattttc aaggcggtga accttgtcgc
    33181 ccgggttgat tctctcgggt gagtagccga ttttaaaatc tacgccgcat ttgagtcccg
    33241 attccttttc aagtacgggg atacatacgt cctcggtaca gcccgggtaa acggttgact
    33301 cgaaaacaac gattgagccc ttggtaaggt tgcgtccgac aatttgcgaa gctccgataa
    33361 cgggagtaag gtcgggagta tggtctgtat ttacaggtgt cggaacggca acaatatgaa
    33421 atttcgcctt tctcaaatcc ttttcatcgg gtgtgaaata tattgttgtt gagcgtatag
    33481 cctcgtcacc gacctccttt gtcgggtcga tgcctgattt gtaaagattt atcttctttt
    33541 cattgaggtc aaaacctata acgttcaagc cttttgcggc gaaagcaaca gccagcggca
    33601 tgccgacata gccgaggccg acaacggaca atgattcttc tttactgata agtttttcgt
    33661 acaaagacat taccgatcac caattccttt tttgttttat tctgcttcaa cagatctttt
    33721 ggctatattg ttgtaggaaa atttttcctt gccgtgagtt tcagcggcat attttatttc
    33781 atcaagcttt tctatagaaa gcttgatttt ttttatgtat tcgtcctcgc tgtcgttttc
    33841 aaagaaaaga cagttgcctc ctatatcaat atgagtgaag ccgctgattc ttttaaatat
    33901 acacggcttg cccatagcga cagcctgctc ccaaagaacc gaatgaagtc ccggaaaaac
    33961 aactatatcc gcagcggcaa attcatcgta aatatcatcg gatttcttcc aacccgaata
    34021 tattacgttg tcgcttaaaa gggagttgaa tttttctttc agctccggaa caacgcttcc
    34081 aaacacggtg agcttgattt taggattatc cagcttgttt attgcatcca tcagcgttaa
    34141 ggtctgcgtc ttattgtggt cgattttacc gcccgttaca agcaatattt cgtcgtcatt
    34201 gattttataa cgcttcctga cagcttccct gaccgccgga tctgaggcct gtgaaaccct
    34261 gtcgtcatcg gcgcccataa caagcagctc gcatttttcc tccgggagac catacacttc
    34321 cttgagaaag tcaactcttg cgggaagaac gccgtaaaac tttgttgtgt aaggctcaat
    34381 acttttggca cagtgcttcc agataattcc gtgaagaatt tttcgtgaaa caaatatcgg
    34441 gctttgccat ttcaagagcc tgactcaatc ctgaaaacct tttaagcttt ttataaaatt
    34501 tatccggaga tttgtagttg agccttgtaa ccaatacatc attttcgttt gtgtagcgct
    34561 ttgcctcgct gtaataacac gtttttccgt cattatcaaa tgtttcaagg gaagcgatca
    34621 cttcaacatc gtaaccctgt tttttatgga atttcggcag catattttct tgatatgaat
    34681 agttatccgg aaaaaatgcg ccaagacaaa gatgaactat tttcatctca gacttcctct
    34741 cgtaaaaaat ctttcggttc accgacatat tcagctcttg cggcaatttc tttgtcggat
    34801 aatttccacc attcgctttt cagaagtttc tcaactgttt cttccggaaa acggtatctg
    34861 ataattttag caggaactcc gccgactatg gcatatggag gtacgtcctt tgttacaact
    34921 gcacctgcgg caacaataga tcctgttcct atcgttattc cgctttttat ctgggcatga
    34981 gcaccaatcc aaacgtcgtt gcctattgtt gtaaggggac aatcttcata agcgacggaa
    35041 gaaaagtttt ttttgagata atttcttccg gagaggaaaa caggcgaggg agaaacaaga
    35101 tttgttgggt gtgacggcat gcctatgttg cacccctctg agattgagca aaatgcacct
    35161 attgatgcgt tttgaatcag cgaatttctt gcaacgtagg agtatttttc cattgtaact
    35221 ccgtaaagcc gtgatccttg acgtacagca gaagtcttat caattttact gttatataaa
    35281 attgccgaaa aggctatgcg ggaattaaaa atacttttaa tttttgaaag tgctacattt
    35341 atcaatgcac tcattttact tcacaacctt ttcaagcgtt tcaacatatt tcttcacgca
    35401 ggtgtctctg tcaaactcgg acataagaaa agtttttccg tttttgccct gcaaaaatgc
    35461 tttttcacgg tcgttataca tttttttgac cgcctcaata aatccgtcaa ggtcgcctgc
    35521 gtcggcaaca agaccgcaat cgttttcttc aatgattttg caaagctcac tttctttatc
    35581 aaatgaggcg agaagcggct tttccgccgc cataatgctc catgttttgc tcggcacgga
    35641 attacttccg acattcggct tgctgattat aagtccgaca tcgccgaggc tgaaaacatg
    35701 tgcaatatcg tcgtacggct ggaacggaat atgaataacg ttctcaatat gctcgtcggc
    35761 tattctttta cctatctcgt ccattgccgc accgtnnnnn nnnnnatgag agtaaaacga
    35821 agctgcggaa tttcgtcctt caaaatcttt gcggcttcaa gcagaagctc catgttttgc
    35881 gtatatccga tattgccgca ataggttata taaaataggc tcgggtcaag tccgtacttt
    35941 tcgataagct tattgccctt tcttccgatc gggtgaacgc cttgagtatc cgcccagttc
    36001 ggaacgaccg taatcttatt ttcgggaacg ccctttgcca ttatattctt tttaaaatcc
    36061 tctgaaataa ctatgatatt atcggcatgg cggtatgtat aattctcgac cgcacgtcct
    36121 attttccaaa tgatgcttcc ttcggatgtc atacccgtat ttacaaggga atcgggaaaa
    36181 atatcctgga gattatatac aaacggtatc tttttgcagg ctttcaggaa tcctgacatc
    36241 attccctgag tcggaggagt gctttgggta aatataacat cggctttttt gctcaagcct
    36301 tttattataa aaatgagatt caaaaggccg taacggaaag cacgcatgag ggaattcctg
    36361 ccctctttat aaagcgcaaa tctgtgaatc gtcagcttgc cgtcgtaaag aatctcagtt
    36421 ttcttttttt tatattcatt tctaacttcg tcgctgactc ctcggcaggg tacgggcaca
    36481 tacatttcaa tgctgtgacc cgcttcagcc atgcctttca gcatatcttc tgtcaggtac
    36541 atgcttgccg caatttccgg ggtatagtaa cagctaaagg ttaaaatttt cattatccga
    36601 ctaatcctct caacgaatgg taatctttat cttatccatg gcttcctcgc atgttttaac
    36661 ggcggcatcg gcgttgctgc cctgagcgat tataaagccc atgcgcgagc cgctgtccgt
    36721 aacgtccgta atcgtttcgc cgacatcgtg aacaatgttt attgaaacga ctccgtcaat
    36781 ttttcttgcc tcttccaagc cttcaatgct ttcaaccgtt cctctatgct ggggaaagta
    36841 gcgtattgcc gctccgcggt caatgataac agagagatcc ggcttttcac ccatggcaat
    36901 tttgatgcag ctttcaacca tattcatacc ggttgagagc ggaacaaggt gggtcgttat
    36961 gcagtctccg ccgagtcttg caccgagttc gacaatcttc gggccgtcct ttgttacaat
    37021 gatttccgta tgtgacggac cgttgcttat tccgacggct ctgttcgctt ctcttgctat
    37081 tcggcttatc tcgtccgttg tttttttatc cagtctgctg ggctgagtgt gacccatttc
    37141 aacgaaatga ggtgcgccgg ttgtgatttt atccgtaatc tgaatgacat gacatttgcc
    37201 gtcaacgctc agagtttcta cgctgacttc ggggccttcc atgaattcct caacaacaac
    37261 cttgccgttc cttgaggatt caaggctgta ttcataggcg ctgtcaacgg cggccttatc
    37321 ggatgaatca gagagtctga tcaccccgcg gc
//
LOCUS       DAWBSW010000002        35878 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_233_length_35878_cov_2.729140, whole genome shotgun sequence.
ACCESSION   DAWBSW010000002 DAWBSW010000000
VERSION     DAWBSW010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 35878)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 35878)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35878
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233_length_35878_cov_2.729140"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4218..4227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6823..6832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8764..8773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14935..14944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15983..15992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16580..16589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19806..19815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    31826..31835
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9341 a         8694 c         7712 g        10051 t
ORIGIN      
        1 agacaccgcc ctttcacggc ggtaacacga gttcaaatct cgtccgggtc accaagtcgg
       61 gatttatccc gactttattt tttgcctgaa aataaacata atcggcattt ataaaagctg
      121 cggaaataaa agtcgtggct ttatatttta tgttgaaaat actttcgtta tgaggatgaa
      181 cccttctctt acattcattc ctgctttgac gggcacacag tctgcgtcct cgtcatacaa
      241 ttttcaatgc aatgaccgtt gctgcatcac ttttttatgg ctgcggaaca gttaaaatca
      301 ccgccacggt cggcaaagca gcggaacaca ttgaattata aataacaata atttaaacaa
      361 caaaacgcaa tcaagttcat ttgccttgac tgcgttttgc gtttttattt tctttttttg
      421 ccgaagaagc ccttcttctc ttcgcccgtc ggggttgaac cgcccatcag cgaattgaat
      481 ttaagcaaag cttcgcgctg ctcggaattg agcttggtcg gaatatcgac aataattctg
      541 acaagctggt ctcccctcgc cttgctgcga atattctgaa tacccttgcc cttgagtctg
      601 aatacagagc cgggctgtgt accctcggga atgtcgtatt taaccttgcc gtcaagcgtc
      661 ggaaccatga gcgtatcacc caaggttgcc tgaacgaggt ttacatggac ctcgcaccaa
      721 acatcaaagc cgtcacgttc aaagaacggg tgcggtctga catttacaac aacatgaaga
      781 tcacctgcgg ggccgccgtt aagacccttg tttccctcac cgcgtacatt gaggatctga
      841 cggtcgtcaa tgccggcagg gatattgacc tcaagcgtgc tctgcttcgt aatgcgtcct
      901 ctgccgcggc actttgaaca cggagtttcg attatttttc ctttgccgcc gcactttgta
      961 catgcacgct gagtctgaat aacgccgaaa ggtgttctct gctgagccgt gacctgaccc
     1021 gtaccgtgac attcgggaca ggttttaggc gaggtgcctg ccgccgctcc gctgccgtta
     1081 caatccgggc agacctcaat acgatttatg tttatcttac gtttgcagcc ctttgccgcc
     1141 tcctcaaacg agatagtaac gctcgactga agatcttcgc cgcgtctcgg accgttcggg
     1201 ttggcacgtc ttccgccacc gccgaatccg cctgcgccgc cgaaaaagct ggagaatatg
     1261 tctcccaaat caaaatcgtc gccgaatccg ccgttgaagc cgccaccgcc tgcaccgaag
     1321 ttcgggtcaa ctcctgcatg accgtattgg tcataacgag cttttttctg ctcatcgctg
     1381 agcacctcat aagcttcgtt agcctccttg aacttcgcct cggcttcctt atcgccgggg
     1441 ttcaggtcgg gatggtactg cttggctttt ttacggtaag ccttttttat atcatcggca
     1501 cttgcgtttt tgtcaacgcc cagcacctca tagtaatctc ttttatcagc cataaattaa
     1561 agcccctgac ttgaaatagg gcaacgccgt gcgaaacggc gtgcccgtta ttagtttatt
     1621 gacaattatt tgttgtcatc gtccgttacg tctgtgtagt cggcatcata gaaaccgtcg
     1681 gctccctgtg cgcctgcacc cggattgccg cccatgttcg gatccgcctg ctgacctgcc
     1741 gcgccctgtg cgccctgagc agccttgtat atcttctccg atacctcata gaacttcttc
     1801 tggagagcct cctgcttgtt cttgatgaga ttgatatcct gacccttgag agcttccttc
     1861 aagccctcga tctcggcatt aagagcgtcc ttatctgcct ggtcgaactt gtcgccttcc
     1921 tcggagagaa gcttctcgca ctggtaaacc atctgatcgg cattgtttct cgtgtcaacc
     1981 tcctcacggc gctgcttatc ctcctgagcg aactgctcgg cttcatgaac agccttttca
     2041 atgtcgtcct ttgacatatt ggttgaagaa gtgatggaaa tcgactgctc cttgttcgtt
     2101 ccgagatcct tagcggaaac gtgaacgata ccgtttgcat ctatatcgaa tgtaacctcg
     2161 atctgcggca caccgcgacg tgccggcatg attccgtcaa ggtggaatac accgagagtc
     2221 ttgttatcct tagcaaactc tctttcaccc tggagaacgt gaacctcaac agacggctga
     2281 ttgtcaactg cggttgagaa tatctggctc ttctttacag ggatagtcgt gtttctgtca
     2341 atgatctttg tgttgatacc gcccattgtc tcgatgccga gtgaaagcgg agtaacgtca
     2401 agaagaagaa gtcccttaac ttcgccgccc ataacaccgg cctggagagc cgcaccgatt
     2461 gcaacgcact catcggggtt gatgcccttg aaaggctcct tgccgatgaa cttcttgatt
     2521 tcttcctgaa ctgcggggat tcttgaagaa ccgccgacca tgagtacctt atcgatttca
     2581 gaagttgaaa ggcctgagtc actcattgcc tggcgaacag gacccattgt agattcaaca
     2641 aggtctgccg taagctcgtt gaactttgct cttgtgagtg taagctcaag gtgcttcgga
     2701 cctgttgcgt caacggtgat gtacggaagg ttgatatcgg aagatgtaac gcccgaaagc
     2761 tcgatcttag ccttctctgc agcttcctta agtctctgca tagccatctt gtcgccgcga
     2821 aggtcaatgc cgtttgactt cttgaactcg tcgatgatcc agttcataac tctctgatca
     2881 aagtcgtcgc cgccggggtg gttgttacct gctgttgcaa gaacctcctg aacgccgtca
     2941 cccatctcaa tgatcgaaac atcgaatgta ccgccgccga gatcataaac cataaccttc
     3001 tgatcgtctt ccttatcaat accgtaagca agagctgcgg ctgtcggctc gttaataatt
     3061 ctcttgacct caaggcctgc aatgtgacct gcatcctttg ttgcctgacg ctgtgcgtcg
     3121 gtgaagtatg ccggaactgt gataactgcc tcggaaacct tctcgccgag atatgcttcc
     3181 gcatcggtct tgagcttctg aagaatcatt gcggaaattt cctgcggagt gtatttcttt
     3241 ccgtcgatag taaccttata atcggaaccc atctgacgct tgattgacga aaccgttctg
     3301 tcgggatttg tgatagcctg acgctttgcg acctggccta ccattctctc gccgttcttg
     3361 cggaagccga caaccgacgg agttgttctt gcaccttctg cgttagcgat aactacaggc
     3421 tcgccgccct cgataactgc tacgcatgaa tttgttgtac ctaaatcaat accaataacc
     3481 tttgacataa tatattcctc ctaagatgtt tatatacact tttatttaag tcaatgccac
     3541 acgcctcatt ttgtgcggtg ttgacttagt tcgcaacggc taccattgcc ggacgaatta
     3601 ccttatcgcc tatcttgtag ccctttgcaa atacatttgt gataacgttc tcgccgagat
     3661 tctcatcctc cgagtgcatt acggcgttgt ggaagttcgg atcaaactcc tcgccctcct
     3721 cgccgaacgg ctcgaccttg agatccttga aaacattttc aaactgtttg aatgtcattt
     3781 caacaccctt tttgtaatcc tcaaaagtaa tatcgggatt agcgaaaatt ctgtcgaaat
     3841 tgtccgccac gggaagcagc tccttgatga ccgaagcctt gcagtcaacg taaagcgcat
     3901 ccttttctct ctgagaacgc ttgcggaagt tatcgtactc cgccgcaatt ctgagaagct
     3961 gatcgctctt ttcggcaagc tgcttttgaa gcttttcctc gttgctttct tccttggctt
     4021 ttttctcttt ctttacctct gcatcttctt ttttatcttc tttctctacc tttttatcct
     4081 cagcctttac ggctgtttct tcactgtttt tcttttttgc caacagtaat cattcctttc
     4141 agtcttcgtt gagaacctgg gagagtattt ttccgaccat atcggtcaga tatctgacgc
     4201 tcggaatcag cgttgcannn nnnnnnnttg catagtaaat cctcgtcggt ccgataattc
     4261 ctatcgagcc gtgattttct ccgccgacat cgtactgcgc cattatcacg cttgaatttt
     4321 caagcggttt aaacttgttt tcgctgccga ttttgacgtc caaatctttt ttattgtccg
     4381 tcaggatctt gctcaacggc tcgccacgcc tcagaaaatc cataagctca taagccgttg
     4441 caccgtagtc gccgtaatgg aagatatttg actgaccgcc gagcataagc ggaatttcca
     4501 tcgcctcgct cgcaagctcc gataccgtcg aaatgagcgg tatcattgtc agcgcatcct
     4561 cacccattga agcaaccaag gtttgaagcg tcgcagtgtc aatagcgtca agctccgtcc
     4621 cgacaaatga agttttgaca atgttgtaga acttctcgat aacggaagcg ttcaaatcca
     4681 catcgctccg acaaactctg cttttgatca taccgtttga ggtaagcaaa acaaccattc
     4741 ccgtcctcgt tccgacaggt acgaattcaa cacgcttaat cgtgacgttc tcgcccgacg
     4801 gagcagttga aattgcggcg cagtttgtca tatccgcaag gaaatcgttt gcccgcttga
     4861 gtatgttttc cggactggcg tttcttatag aaataccggc ttcgatacgt cttttgtccg
     4921 catcgtcaag ctgccgcact cccataagat gatctacata gtaacggtag ccctcgtcgg
     4981 tgggaattcg acccgccgag gtgtggggct gttcgagaag tcccatgctt gaaagctcag
     5041 ccatctcatt tctgatggtc gccgacgaaa ccgaaatctc gggatccttt tgcaagagct
     5101 ttgaaccgac aggctcgccc gtctttatat agtgttcgac aacagcagca agtatttttt
     5161 cttttctctg actgagttcc ataatctcac cgcctttagc accgttagca ctcttttgcc
     5221 atgagtgcta actacattta atactataca accgtccgac cgatttgtca acactttaat
     5281 tgtgaattct ttgttaacat ttgcagtttt tccattaaca atgattgcag acgaacttga
     5341 ttaacggagt gatacataat ttctgccact ttccatcttt atccccctca gcgcactaca
     5401 ctgcgggctc tgtcggcgga ggagcctgtg ttacaacgga ctttattcag accttgggtc
     5461 gatgtgggca tcgaccctac aaactgtgct ttttcaaatc acaataaaaa tgcaatatca
     5521 aaaataataa aaaattttgc atatctatac cttataatat attttgacgc agcaggcagg
     5581 attattctgc atttccgcac attacctgtt gcttttacga aaaagatatg atacaatcgc
     5641 caatgtcagg aggtagtgaa aatgataaga aaaattatta aaatagacga agaaaaatgc
     5701 aacggctgcg gcgcatgtgt ttctgcatgt cacgaaggcg ccattggtct tgttgacggc
     5761 aaggcgaagc ttatgcgtga tgattattgt gacggactcg gagactgtct gccctcatgc
     5821 cctaccggtg ccatttcctt tgagcagaga gaagcggcag actacaacga aaaagccgtt
     5881 atggaaaaca ggctcaagaa gcacggcatt gctaatcacg gcggatgccc gggctcaaaa
     5941 ctcagccgta tcatgcacga tcacggctcg catacagata taaaagctga aagccgtctt
     6001 tcgcagtggc ccgttcagat aaagcttgtt cccgtcaatg ctccctattt tgacggtgcc
     6061 gacctgctca tcgccgccga ctgcacggct ttcgcttacg gcaatttcca caatgatttc
     6121 atcaagaata aaattacgct catcggatgt ccgaagcttg acagcgtgga ctattccgaa
     6181 aagctgacag ctattttggc agttaacgac atcaagagtg ttaccgtagt acgcatggag
     6241 gtgccctgct gcggcggaat tcaaacggct gtccaaaagg cacttgacgc aagcggcaaa
     6301 aacgttcctc tcaacgtcac ggttatttcg actgacggta aaatcaaaaa caaataaaat
     6361 ttttcggaac tgcggcaaat aagcggttcc gaaatttttc tataatatca caatgtataa
     6421 cacaagcacc aataaagcat ttttttatct ttcttttttg cctaatccaa tgatataata
     6481 tttaaaaacc gtcttattcg gcaacatttt attctttttt gtaaaatatt ttgctttttt
     6541 tcaatatttc cctttgcctt gtcacttgac aataccgtta caaggtgtta aaataggttg
     6601 caataagatt gatacgggag aacatcccgt ccaccgaacg tcgatcgcat tgcgattgtg
     6661 ttcggctttt atttttttcg gaggtatcat gaaaatgaaa acagacgttg taatcgtcgg
     6721 agcaggccct gccggtatat tcacagctat cgagatgctt cgcaagggca ctaagaaaaa
     6781 gattgtcatt gtcgaaaaag gtcagtcggt cgaaaacaga cannnnnnnn nngtcccaag
     6841 gcgaagacta aaaaatgtat gaattgcaaa ccgtattgcc acattactac cggcttttcc
     6901 ggcgcaggcg cattctctga cggcaagctg tcgctgagct atgaggtcgg cggagacctt
     6961 ccgaatcttg tcggagaaaa actggttcag gaaaccatca actataccga cggtatatat
     7021 cttgaattcg gcgcagatac taaggttgag ggcgtcggaa acaccgaaga ggtcagaaag
     7081 atccgtaccc gtgcaattca ggcaggactg aagcttgttg actgtccgat ccgtcatatg
     7141 ggaacggaaa aggcacagga tatctacctt gcaatcgaaa aatatctcat tgcaagcggc
     7201 gttgagatct tcttcggcta tgagtgctct aacatcatcc taaacggcaa aaagtgcgaa
     7261 ggaatctatg tttccaacaa caagggcgtt gaaatggaga tcagagccga caatacgatc
     7321 attgcaacgg gacgcagagg cgcagaatgg cttgaaaaaa tctgcggcga gcatgatatc
     7381 gctcatttac ccggaacggt tgacatcggc gtaagagtcg aggtcagaaa cgaggtaatc
     7441 gagaaggtca atgacgttct ctacgagtca aagctcatcg gctatccccg tccgttcaaa
     7501 aacaaggtca gaaccttctg ccaaaacccc ggcggctttg tcagccagga gaattacgac
     7561 aacaatcttg ccgttgtcaa cggtcattcg tataaggagc tcaaaagtga caacacaaac
     7621 cttgccattc tttgctcaca taacttttct tacccgttca atcagcctat cgcttacgct
     7681 cagaaggtcg gcgagctgac aaacatgctc ggtacgggtc acatcctcgt tcagcgcttc
     7741 ggcgatattc ttgacggtaa gcgcacatgg gaaaaggagc tttcgcagtc caacctcaag
     7801 cctacgcttc cggacgcagt tgcaggcgat atcacagccg caatgccgta cagagccatg
     7861 acaaatatta tcaactttat tcaggctgtt gaccatgttg ctcccggctt cgcttcctac
     7921 gaaacacttc tctactctcc cgagctcaag ttctacagca acagagtcaa gatggacgag
     7981 cacttcaaca caaacgttga gggccttcac tgcctcggcg actcaagcgg ctggacaaga
     8041 ggccttatga tggcttccgt catgggcgtt ttgatgggca gagaaatcta ttaaattatt
     8101 gcaagtaata ttttaatgcc gcaagaacgg agatgctgcc atgaaagtca cactcaatcc
     8161 cgacaaggag atagtcaata tagtcaaaga aggtctcaag cgcacgggcg gctactgccc
     8221 ctgccgcaga gagcatacgc ctgagaacaa gtgtatgtgt gaggaattca gaaagcagat
     8281 tgaagatccg aattttgaag gcttttgcca ttgtatgctt tattataagt caaaggacta
     8341 aggaggtttt tatcatggct gaagttaaaa tcacaaaaga caattttaca gccgaggtac
     8401 ttaaatgcga tattccggtt ctgctcgatt tctgggcgga atggtgcggt ccgtgccgta
     8461 tgctcgctcc gatagttgaa gaaatagcgg acgagtacga cgatgtagtt aaggtcggca
     8521 aagtcaatgt tgacgaagag cccgagcttt cctcgatttt caacgtttca agcattccga
     8581 caatggtcgt tatcgaccac ggcaaggcga ataaatccgc agtcggatat atgtctaaag
     8641 aaaaagtcct cgagcttctc ggactgtgaa ataaaacaaa atcggcagcc tgcattttca
     8701 gcagactgcc ttatttttta attatatgtg cttttatcga actgcttctt gaaatattcg
     8761 tcannnnnnn nnnctctcgg cgttttcggg aacattctct gtgcttcttg agcggcgatg
     8821 tcttttgccg tcgggcgact gtttttgctc tgtttttgct ctgttttcgc gacgtggttc
     8881 ggaataaatt tccgccacac cctttgaaaa tgccttcggc tctctgtcaa aatcaagaat
     8941 ttcattttcc ttaattccgc cgaatctttt cgcttgctcg gtctcgttca tcatcataag
     9001 atagttattt gacgcctttt tttcgtcaag ctctgtttcg gattcgttca atgccgaaag
     9061 gacatcatga cgtgttttca tttctttttc atattctgca ttgactctgt cacgtctgta
     9121 atagaacgga ataaagatca cacacgaaat taactggata actaatgact tcacaagcag
     9181 ccacttatcc ggcaagcggt cgggcgaaag ataaatgcag gtcattatga acgcattgac
     9241 aaaatgttcc gcaatcgcca tccataccga gccgttgacc ttgtaataat atccccaacg
     9301 tattgagctg aaaaacacag cagccgcaga tgccgaaatt gttataacca cgtccgcctt
     9361 cggcatattt ttaaatccgc cctcggcaaa agctccgatt gcgggcaaaa atgacagtgc
     9421 ggtgaagatt atcgactgga cataatttga ctttttctca ccgagctttt tgcaaagccg
     9481 attgagcata aagcccctgt aaagctcctt gaaaacgacg ttgacgatca aagcaaaggc
     9541 ataagcggca atcgccggaa tcaatccctt ctccgttgaa ttcggcggaa gaataaaaat
     9601 acttatatcc gaatagaatc ccttgatttt gaaataaatc agttccgcag catagataag
     9661 cacaatcggc gcaagcgaaa agccgatgcc gtaaagcagc tcgaaaaacc agccgaagcg
     9721 acctaagcat ctcctgccaa tattgaactt cagcatcttt gaagctgcaa gaaccgcaaa
     9781 cagtcccaaa actcttgcga tcacggtatc gccgaagatt gtattatcgc tttttagaaa
     9841 gaaaacgtca atcgcttgaa ttataaaaat aaatgccgtc agaaaaaaag tgattgtaat
     9901 tcttttttct ttcaaatctt tcataatttc ttctcgttcc gttttatttt atctcttcaa
     9961 tctccctgag ccaaatcgca tacgaagcgt cgctcggcat tctccaatct cctctcggcg
    10021 aaaggcaaac gctgcctatc ttcaccccgt ctggcaggca gctccttttg aactgctgag
    10081 tgaaaaatct cttgtagaat accttgagcc atttaagtat cgtttccctg tcaaaatcct
    10141 ctttgaatgc tttctcggcg aggaagaata tctttgaagg cgaaaatccg aagcgaagaa
    10201 gatgataaag gaagaagtca tgcaatgcat aaggtccgat tatctcctct gtctcctgca
    10261 tgatattgcc ctcctcatcg ggaggaagaa gctccggact tatcggcgta tctattatat
    10321 cctcaagcac atgagtgctg tcggggaaaa tattataatc aatcagcgcc gctatcatcc
    10381 agcgcacaag ggttttcggc acaccgcaat tgacgccgta catactcata tggtcggcat
    10441 tatatgtgca ccagccgagt gccagctcgc tgaggtcgcc cgttccgaca acgaagccgc
    10501 cgaccttgca ggcataatcc ataaggacct gcgttctttc acgagcctgg atattttcat
    10561 atgttacatc gaatttatcc gtcgggtgac cgatatcacg gcagtgaagc gtgcaagcct
    10621 cttttatgtc aatctccatt gcgtgaacgc cgagagtctc cataagctcc caagcgtttg
    10681 aatgggtgcg tttcgtcgtg ccgaagcaag gaagcgttat cgcaataacg tccgagggat
    10741 cgcgtccgag ctgacgtgcg gtttctgcgc agacaagcag cgcaagagtg gaatccagtc
    10801 cgcccgaaac accgagaacc atttttcctc cgacctttga cgccctcttt ttaagcccgg
    10861 cgacctgcat acggaagata tccatacatc gttcgattct gtccttctta tccgagggaa
    10921 caaacgggag cttcttcact tcgtatatct ctccgtcgct ttcaagctcc gctgctgtct
    10981 cgacttcaac ataatcctta ccctcaaggt aaagagctgc cgagtccgca aacgtcttgt
    11041 tctctgtccg gtccgccctt atcttgccga gatcgacatc atgctttata gaatatcctc
    11101 cgtcgatcag gttgtcatta tgagaaagaa cggcaccgtt ttcgcatata agagaatgac
    11161 ccgagaagat aagatccgtt gtcgattcgc tcatacctgc gctcgcatag gcataggcgc
    11221 aaaggctcct tgccgactgc tgagaaacaa gctcggttct gtattttctt ttcattatag
    11281 tctcgtttga ggcggaaaga ttgagaatga tctccgcacc tgcaagcgtc agaagcgttg
    11341 acggcggcaa aggcgcccaa aggtcttcac aaatctcgac tccgattttc actcccgagc
    11401 agttaaacac aaggttgttt ccgacgggaa tttcataatc gccgtcaagc ataagctctt
    11461 tcgaactgat tgaggcgcat ttcaaatcag ccgcagatga aaaccaacgc ttttcgtagt
    11521 attcgttata ggtcggaata aaggttttta cggcgatgcc gttcaccatt ccgtgtgaca
    11581 tcacaacggc acagttataa agctgaccgg cgataataag gggcgcaccg actgcgacaa
    11641 cggagttgct gtcctttgta gtcctgacta tttcggcaat gcccttcttt gcgccgtcaa
    11701 tcagagtttt ctgaaagaaa aggtcggcgc aggtgtagcc cgtaacgcaa agctcgggga
    11761 acactatcac atcggcgtcc ttattgatat cgatatactt aattatttcc tgcgtgttga
    11821 attccgtgtt gccgacctcg acatcgggaa ccgcacacgc caatctgata aaattaaaca
    11881 tgtttatcac cttaccgtat ttatacataa tttattataa cataaaattg caaagtgtca
    11941 aatatcttgt tgcaaatgtt gcatgacttt tattttgata aaatttattg aaattttcgt
    12001 cttaatatgt taaattatat gtaatatata caattagata gagaggaaca cggaaatgca
    12061 aaaatattta aagacaaaag agcttcattt cggctcgaag actttgaagc tgccctccga
    12121 ggcgctttcc tacggaatag gacttttcgg cattgccctg ctctcgggct gggttcccga
    12181 atatgcaaaa acctactttg acgattttgc attcaaaaat caaaatttcg gttcaaccgc
    12241 tcttgcaacg ttgatatcca ccgtttttct cgttgcagga tttgtcggag cggcgatgga
    12301 aataataata ggctttcttg ttgacaagac gaaatcaaag cgcggcaaaa ttcatccatg
    12361 gcttctctac ggctgcgttc cgctcggtat aatctcgatg ctcgttttcg ttgcgcctaa
    12421 aacgtcgaat tttatcgttg caacgatttg gatgttcgca atttactgtg catatgtaat
    12481 gctgctcgtc tgcgtcgaaa gccccggtaa ctgcttcgga gccgtctgta cgccgaatcc
    12541 agatgagcga agcgacgcaa tttcaattgc aggaattatg cgctcaatcg gtcaatccgg
    12601 cggaatggtt gttctcatcg tcgtcggtct tgtcatgaaa gctatcatgg gtaatgaggg
    12661 cttcaaagcc gccgagggca gagggcttga tctgcagatt tcaacagccg tctgcgttat
    12721 cggcttcatt cttttccttg taatcatgct cagaaacaca agggagcgtg ttccctacac
    12781 aacggagaaa gtcaatctcg gtcaaagcct taaatttgtt ttcaccaaca agaatttgct
    12841 catggtttct cttgccaagc ttgcaggctt cggacgcggt gtttacggtg cggcaagcct
    12901 ttatatcgca atatatcttc tcggcgataa aggtctcaag cttgcacttc ttctcccgat
    12961 gggaatcggt acggcggtca gtatgctcgt cgttaaaaag ctcatcaaaa ccataggcac
    13021 aaagaacact tttatcgctt gcagtgccta tggcgcagcc tcgttcctgc ttctattcgg
    13081 cgtttcaatg ataatcggat tcaggaagga gcttattatc cccttcctta tcatcaattt
    13141 ccttgtcgga cttcagaacg gaaacaccaa cctcacgccg aatgtcatga ttgccgactg
    13201 tgttgatgaa atcgagctga aaacaggcaa gcgtcaagag ggtctctgct acgctggcta
    13261 cggactgttc tcaaagattg cttcggcatt cactacctct ctcggtacat atcttgtttt
    13321 caaatggtcc ggttatctcc cgtcaacgga ttcgtcggtc gcttatgcgg ctcagagcga
    13381 cggcacactt acaagatttc ttattatcta cacgataatt cctgcttgct tcgttgttct
    13441 ccagataatc ccgatgcttt tctatgacct caacgcaaag aagaaggagc agatttcact
    13501 tgaacttatc aagcgcagag gagttgttga aaccgatgaa tgatacggca tttgaatatc
    13561 cgctcgactt aattataacg actgacgctc actactattc accgaagctc ggcatagata
    13621 ctccgtcata caaaaaatat gattcaacca atcaaaaggc tgtaaaggac agccctgcga
    13681 taatctccgc cgcattcgct cagattgccg aaagcaactg tgaaaacgtt attttctgcg
    13741 gcgatgcaac ctgcgacggc gactatgaca gccatgttga atttatagag ctgctatatg
    13801 ccttgaagaa gtgcggcaag cgtgtttttg cgataacctc aacgcatgac tatcaggacg
    13861 acggactgac aaactgctac acgggcgatg aaaaaacaaa agttccgtcc gccaagcgtg
    13921 aggagctatt aaaaatgtat cacagcttcg gacccgatga ggcgttctcg cagtttgaga
    13981 tgtcctacgc cgccgagctt gacgaaaact atattctcct tgccctcaat tcggacaaaa
    14041 acggcaaggg caagagcggt tattccttag agcataaggc atggatagcc aaaatcgcaa
    14101 atgcggcaaa gcaatgcaac aagcgtgtga ttgcttttac tcatcacccg attctttcgc
    14161 cctctcccgt ttattcactg ataggcaaga acgacatgat gggtgaacac gaagagatta
    14221 aagacttcct tgccgacctc ggcatatgtc ttgttttcac gggtcattcg catgtgcatg
    14281 atatatcata tgatttttca ccgagcggaa atatttttta cgacgtttca tcctccgccc
    14341 ttgccggcta tccgggtctt tatcgcacgg taagaatttg cggcagcaat gttcacattg
    14401 aaagcgttgc gataaccgaa agcgtcagcg gcatcagcaa tttgcccgag tatcttgaaa
    14461 ataaattcat aggaatgatt gacagaacgc ttgatgcagc ggcctcggac attgatacat
    14521 ttgccgcctg cgtggattca atgagtattc accccaatgt ttcctatcga ataggctggt
    14581 ttttcaggcc ttttgcacgg cttatcaaaa agcttaagct gggtgttttt gcaagatggg
    14641 taaagagcga aacgcataat gccgaccttt ccccggctga gaacgagagc gtgttcgact
    14701 tgattcgtca gctcgtcctg cacctgtatt ccggcaacgc tccctatacg cccgacacgg
    14761 cagaatacaa aattgtaatc ggctttgtca acatcatcga cagcgtgctg aagcttcttc
    14821 atatcaaaat gaggctctcc gaccttgtgc gcccccttct ctacaacagc ggaattgacg
    14881 acagcaacgc cgatctgccg ctgaacgcag acaaaaaagc tgtcaataca atctnnnnnn
    14941 nnnnccgctt attccggtca tagccgttgc gtttttgatt ggcgcaggag tcaatgaaat
    15001 tatatatcga aaacaaatac gaggtaaata gtatgaacaa tcctttaaaa gccgtcatag
    15061 tcggtggcgg ccatcgctcg atgatatacg ccgggctggc aaaaacaaat cccgaaatgc
    15121 ttaagatagt cggtgttgtc gatccaaata aatttcgacg taatcaaata ggtgaatatt
    15181 ttgatattcc gagtgagtat cgctttgaga atgtcagtga ccttgttgcg ataccgaaat
    15241 tcgccgatgc cgctataaac ggaacgatgg atcacatcca cgttgcaaca tctatccctc
    15301 tgctcaaagc aggctacgac attctgcttg aaaagccgtt tgctgtcaat gaagaggaaa
    15361 tgcgtgagct gataagctgt gccgaggata acggacgcaa aataatgatt tgctttgttc
    15421 tcagatacgc tccgttttac cgccggataa aagaaaccgt cctcagcggt gaaatcggac
    15481 agatagttaa tattcaaacg gtcgaacacg tcagctacca ccatatgtct acaagccata
    15541 tcaggggaaa atggcgaaac gatgagcagt gccatgcaac tatgcttctt gcgaagtgct
    15601 gccacgatat agacatcatg atgtggctca tgggtgatga cgcacctgca aaagtatcga
    15661 gcttcggttc tcttatgcag ttcagaccag aaaacgctcc aaagggaagc ggtacacgct
    15721 gccttgttga ctgtcctctt gttgacacct gcctttactc ggcaaagcgg atttatatcg
    15781 accaccccga tcgctggtca ttctacgttt gggacaagct tgaggacagg gaaaacgcaa
    15841 cgctcgagga caaaatcgag cttctcaaaa caagtccata cggcgtttgt gcctacaaga
    15901 gcgacaacaa cgttgttgac caccagagtc ttgccgtccg ctttaagagc ggtgcaaccg
    15961 gcacgcataa catggttgga ggnnnnnnnn nncatataat cggtacaaag ggcgagatct
    16021 tcggaacact cgagagcgga aaattcaccg tttcgctcat tgacccgaga cccggttgcg
    16081 agcatcttga ccgtgttgtt gatgtcaatg taaccgacga ttctcacgga ggcggagata
    16141 ttgagcttgt caaggatttt gtacgctatg ttcgtgacgg tgtaatgtct gtttcatgca
    16201 cctcgattga ggactcgatg aagggtcatc tgaccgtttt ccgtgccgat aagagcatga
    16261 aaaacgacgg cgaggtcgaa aatgtaattg aatagcgaat aaaggtcatg ccttttcgta
    16321 tataatataa gtgtaatgca gaaaatattc ggagctgaat tgttttatag taagtaaaat
    16381 ttctaccgac atttatcagc tccccgcagg agcccacgct caacggaaat ttctgccgat
    16441 atccaacaac atttatacga aaggcgtgat ttttttgacc gtcaagacaa atatcaattt
    16501 caagcacctg ataatcgcag ttgctattcc ccttgccgtc ggcggaatat ccggccacgt
    16561 gtggcgtggc aatcagtgcn nnnnnnnnnt atcatcactc atagtcggca gcggcttcga
    16621 tgtttattcc gaagcggtca caaagccgcc catgtcccct cccgattggc ttttccccgt
    16681 ggtgtggacg ttactttaca ttataatggg gatttcttct tatctcgtgt atgaaaagga
    16741 caaatcggaa gccaaacagc ctcttgcggt ttatgccatt cagcttgcaa tgaacttcat
    16801 gtggccgata ttcttctttt cgcttaatgc ttatcttttt tcatttatat ggctaatcgt
    16861 tctttggtca cttgcgatca ctatgaccgt catgttttac cgagtttcaa agcttgcggg
    16921 aatgctccaa atcccatata ttctatggct cacatttgcg gcttatctca acctctcggt
    16981 gttcctgctc aattaaaata ttaacaaatt attaaatttt taaaaaaata ttgactattg
    17041 gtcatttttg cgtataatgt taaacgaaag caaaagtgac cgatagtcat tttattacgg
    17101 agatatgcaa aatgagtaag atatctgaaa aacaacagaa taaaaggacg agtattctcg
    17161 atgctgccta tgagcttttt attgaaaaaa gcttcaaaaa cacctcgatt gacgacgttg
    17221 tcaagagtgc aggaatagcc aagggcacgt tttaccttta ttttaaggat aagcatgacc
    17281 tcatggagcg tataatcgtc cgaaagtgtg ctttgattct tcgctatgtt cttgaggaat
    17341 tgatgaagaa aaaagaggcc tgccggatgt cttttcccga gcagatgctt tttataacag
    17401 aaaaaatcat tgattatctt gaagatcata aagaaatcat taccctcata ggaaaaaatt
    17461 tttcctcatg cctgagctat ttcagcacga ttgaggatga ccggcttaaa tcaatgctca
    17521 gcgatctggt tcacgaaaat ctcgaaaacg gtttttccga acatgaaaca ctcaaaagaa
    17581 tttatcttat cgtaacactt gtcggctcgg tttgctacga ctcgtttgtt tttgaatcgc
    17641 cgttcaagat ttcggaaatc aagccgctgc ttttaagaag cgttgaaaac ataatttccg
    17701 aacctccgac agacgaaaaa aataaatcag tttaataccc ttttaatctt tacatattac
    17761 ttgtaaagta ccatataaaa atctaaaaaa ggagatcggt tatagatgaa aagaatagca
    17821 aaatttattg cctaccatcc gaagacgata acgattattg ccgtcttgct tctcattccc
    17881 gctgcaatcg gctacatcaa caccttcgta aactacgata ttctttcgta tcttccgggt
    17941 gacctcgatt cggtcaaggg cgaaaatgag ctggatgaga ccttcaacag cgcctcaatg
    18001 tcattccttg ttattgagga tatgccttcg aaggacgtcg cagccttgaa agaaaaaatt
    18061 gcaaaggttg ataatgtaag cagtgttatc tgggtcaacg atatcgcaga tataagcatt
    18121 ccgcaggaag tcattccgca ggccgtcaag gatgtttttt acagtaaaga cggcaaatgc
    18181 acaatgatgc ttatacagta taaatacaaa gcggtcaccg atgaaaccat gaaagccatt
    18241 gaagatgtca gaggcttgct caacaatcag tgcttcctca gcggtatggg cgtcattatg
    18301 aaggatacgc gagacctcgc agattcgcag gctccgatat tcattgcaat cgctattgca
    18361 atcgccctcg ttgtcatgac cttctgtatg gagtcatggg ttgagccttg ggttcttctg
    18421 gcagcgctgt gcatggcggt tgtttacaac atgggcacaa acctcatatt cggtcagata
    18481 tcgtacatta cacaatgtat cgccgcaatc ctgcagcttg gcgttacgat ggactattcg
    18541 gtatttctca ttgaccgatt tgaagaggaa aagccgaaat atgctgacaa gagagacgct
    18601 atggcaagcg caatccaggg cgcatttctt tccctctccg gcagttcgct gacaacaata
    18661 ttcggtttcc ttgcgctctg cttcatggat ctttcactcg gcaaggatat cggtcttgtt
    18721 atggcgaagg gcgttgtatt cggtgttctt accgttacga tcgttctccc cgcccttgtt
    18781 cttcagtgcg ataagattat tcacaagtat cgccacagaa gcttcattcc aaagtttgac
    18841 aagctcaaca attttgttgt caaccacaag aaggtttggg ttgcaatttt cattgttctc
    18901 ttcattccgt catacatcat gcagtccaat gtcaatgttt actactcgat ggacagaatg
    18961 ttgccggacg atatcggctg tatcgccgct ctcaacaaga tgaaagagga gtttaatatg
    19021 gcaacctcac acttcatcat tgttgacgac agccttgacg cctctgacct cgttaagatg
    19081 gagaatgaaa tcaagggaac cgcaggcgtt acaaacatgg ttgcatacaa cagcttcgtc
    19141 ggttcgggta ttcccgattc aatgattccc gatgagctga agagcatagc aaagtccggc
    19201 ggcagacaga tgattctcgt caacacctca tacgttgcag caacagacga atgtaacgaa
    19261 cagctaacca agataaataa tattgttaag aaatacgata agaacggcta cgtcaccggt
    19321 gagggcgcaa tgtacaagga tttgatcgac atcaccgacc acgatttaaa gctgaccagc
    19381 gtgctttcaa ttctttcgat attcattctt atcgcaatca tctttaagtc gatttcgatt
    19441 ccgacaatac ttgtaatgtc gattgagctt gcgatattcg tcaataaggc tatatcgttt
    19501 gcaacgggca cgacgctgtc gttcatagcc ccgacaatta tcggctgtgt tcagctcggt
    19561 gcaacggttg actacgcaat tcttcttacg acgcgtttcc gtgaggaaat gagaaacggc
    19621 cacaacaagc tcgacgcaat caagattgcc gccaacgctt ccgaccgttc gatattccag
    19681 agtgcgctcg tattcttcgg tgcaaccttc ggcgtattcc tcacctgcga tatcaagata
    19741 gttaaagagg tttgtctcct gctcgcaaga ggatctctta tcagcgcagg cgttatagta
    19801 ttcatnnnnn nnnnnccgtt gctgcttgtg cttgagggtc tcatcgagaa aacgacttat
    19861 gacttcctcg gcaaaaagct caagcttaag atgaaaaaaa acgaaaacaa aacggcagat
    19921 aacaatgcct gaacaatacg gaggtaataa tatgaaaaac aatctgaaaa aaatcatgtg
    19981 cgttcttctc tgcgcttcta tgcttgcatc cttctctgcc tgttcaaaga agaatgacgg
    20041 cagcacagac gaaccgacca cagctccgag tcagtccgac acaacaaagg taagctacga
    20101 caccgccgca acctcataca agaaaaatga gacagtttat gtcaacatgg cttcaaacgg
    20161 cgaggttaca agcaaggtcg taacagactg gcttcatacc gacaaggctc agacctatat
    20221 cgacgacaag accgaccttt ccgatataaa aaacgttaaa tccaacgttg aacccgttaa
    20281 gaacaaggac ggttcatatc gctggaacat ggaatcaact gacctctact acagaggtac
    20341 aacggataag gagcttcccg taaacttcaa gataacctac taccttgacg gcaaggagac
    20401 cgaagcaaag gatatcgcag gcaaaaaagg tcaggtcaag atggtagtta ccgttaacaa
    20461 cgagtccaaa aagaccgtta aggtcagcgg taaagataca acaatctata ctccgttcat
    20521 cgttgcaggc ggaatgattc tcccggagga taatttctca aatgttaccg ttgagaacgg
    20581 aaagaccatc ggcgacggaa caaaagaaat tgctctcatg gtcggcgcac ccggactcaa
    20641 ggagagcctc aacctcagcg atgagatgct caagcagctc ggcgatttca acttctcaaa
    20701 cacttacaca attacggcag acacggaaaa atttgagctt tcaaacatga tttttgccgt
    20761 tcttcccctc tcggctatcg agagcagcgt tgagaacaac ctcccgaaca ccgtttccga
    20821 tgtcaagaac acactcaatg aggttcaagc ggttattgac aagttcaaca gcatgaatgc
    20881 aacagagctt atcaacaagc tcttctccaa tacagataac cttacggagc tcgcctcctc
    20941 tgtttccgat gttacaaagc tttataacga caacaaggct ttgcttgacg tactcgaaaa
    21001 gtacatgaca aaggaaaata tcgcagcaat tgaaaagctc attgccgata ccgatgatac
    21061 agatcttgag caggtcgcaa agcttctcaa caatcccatt cttcagaagt tctttaagca
    21121 gctcccgacc ctttcaaagg atatggaaac cgtaatgcct atcatcaccg gactttcagc
    21181 ggatatgcag aagcccgagg ttcagaaggc tcttaagaat cttccgcaga cgattgaaac
    21241 acttaagaag ctcaaaacaa cgattgacaa gaatcaggat ttgtttaaca ccctcggcga
    21301 aactcttgat gacgacacaa tcgcatcact caagggcatc atgagctcac ttgataagat
    21361 tatttccgag aatgaccttg aagcttatgc aaagcttgcc gacaatgccg acgatctgat
    21421 tgctcgcgca aaggcatgga tcgaagcagg tcaaaagtac gacatcttca caacaaaggg
    21481 tgccgcttca agcacatcgg taatgtttgt ttatgagaca gccgctgttt ccgctccgag
    21541 tgaggaggtt aaggaagagg cagagactgt tgaagaaaac gcaattctta aatggttcaa
    21601 gaatcttttc aagaaagata aataatccgg cagatgcaga aaaagcctca cgaagttttc
    21661 gtggggcttt cgttttgtac actctcaaaa ataatatccg ctattgcctc ggcgttctcg
    21721 tcgaggcttt tatcaaagct tttattatca acctcgtttg tcacgaagcc gacctcgaga
    21781 agaatcgacg gcatatttgt attggaattg acatagtaat tgtttccgct tgagttgcga
    21841 taaccctttt tcactcccct gttcggcaga ctcgaaatgt cactgagcgc ctcaaccagc
    21901 ttttgcgcgg tctcctcttc gttgcccttc ggctcctttg ctatccacgc ctcaaagccc
    21961 gatcccgaag ccgaactgtt tctatgtaat gacaggaaaa aatcacatcg ttttctgttt
    22021 gctaatttgc agcgctcctt aagcgtaata tcggaatcgt cctcacgagt caaaataacc
    22081 tttattccct ttgcctccag cctgtctttc accttgagcg tcaggcgaag attatcatcc
    22141 ttctcaagcc ttgctccgcc cgttgaggtt gcgccgacat cgtcggcacc gtggcctgca
    22201 tcaaggcaga cggttttgac actttcataa tgctcctcaa ccacgcccga caggaatttg
    22261 accgccacaa gaaagcttgt cagaacaaga cccacggcaa taacagccgt aattcccgtt
    22321 tttcccttca atatattcat ttttctccct cttttttgtt actttctaaa aaaaacaatt
    22381 gactttatcc ctgtttggta gtatattgga ataagtatat gtttacggag gctgaaataa
    22441 tgctcgataa aaatttcaac ccgtctctgc gattcatggt cgtcagcgac attcacatta
    22501 aggatgaaga gtgcattgag gaacagcgct tcgcttctgc tctgaaatat gcttattcta
    22561 ttgctcaaag ctccgagaat tacaaaaagc ttgatgcaat cgtcattgtc ggcgactttg
    22621 caaactcggg aacggaaatt cagatgcaaa aggtcaggaa cattcttgac gcaggcgttg
    22681 attatgacga aacgcaggtt atatcctcgt ttgcaagcca tgaaacaaac agcgacggta
    22741 cagaggttgc gaccgaaaga atgaaacgta ttctcggtca ggatcccgat caacacaagg
    22801 tcatcaacgg cttccatttt gtctccatct caccgtcacg cagttgtaac tatgatgacg
    22861 gcaaaaaggc ttggatggca cgggagcttg cttctgccgc aaaagatgac agagctaagc
    22921 cgattttcgt atttcagcat cctcacaaca ccaacacggt ttacggaagt attctttggg
    22981 gtgaggacga gctgataaca acctatatga actatcccca gataattcat ttctccggtc
    23041 actcccacgc tccgataaat gacccaaggt cgattcatca acagcatttc acctcgctcg
    23101 gcacgggtac gctcagctat tttgagatgg atgagttcga caaaattaca ggttcaatac
    23161 ccgataacaa agaaaatgca gctcaaatgc tcattgttga agctgacgca gacaacagag
    23221 taagggttta tcccttcgat gttctgacag gtcatttctt cccgcaggtg cgtaaaatcg
    23281 acactccgag cgatataagc tcctttatct acaccgccga aagatataag acaaaggttg
    23341 tcccctattt tgccgatgat tcgaagctga cggtttccga tgtaaccgac acatcgttta
    23401 aaatagaatt tgatcaggca agaattgacg aggattatgt cgactgttat gatattgtgc
    23461 tcaaaaatgc agacggcttt gtcatcagac atttaagtat atggtcggaa tattattttt
    23521 atgatatgcc tgaaaaaaga tcttacgtat tcaccgatct tgaaccaaaa acaaaataca
    23581 cggtaactgt caaggcgtgc ggcttttgga acaacatttc cgaaaacagc cttgatgcgg
    23641 atataaaaac attataacaa cacgggaggg gtctcaaatg tcaaaattca aagcgtttct
    23701 caagcgcaag gacatcgaat tttccgccaa aagatacgga attgatgctc tcggcgcaat
    23761 ggcgcagggt ctttttgctt ccctgcttgt cggcacaatt ctttcaacga taggaactca
    23821 gttttctatt gaagcacttg taacaatcgg cgattttgca tcggcagtca agggctgcgc
    23881 aatggcaatt gcaatcggat ttgccctcaa agctccgccg cttgttctct tctcgctcgc
    23941 aaccgtaggc tatgccgcag ataagctcgg cggtgcaggc ggtcctctcg cagtattact
    24001 cattacaata gtagccgccg aattcggcaa agccgtaagc aaggaaacaa agcttgatat
    24061 tcttgtcact cccgttgtaa cgatcggtct cggctgtgtg ctctcaatgc tctgtgcacc
    24121 gtatataggc aaggcggcaa gtgcagtcgg tcacgctata atgtgggcaa ccgagcttcg
    24181 tccgttcctg atgggaatta ttatttcggc tattgtcggt atcgcgctca ccctaccgat
    24241 aagcagtgcc gcaatttgtg ctgctctcgg acttacgggt cttgccggcg gagccgctct
    24301 tgcaggctgc tgtgctcaga tgataggctt tgccgtcatg agctttcgtg agaacaaatg
    24361 gggcggactt gtttcgcaag gtctcggcac aagtatgctt caaatgggca atatcgtcaa
    24421 gaatccccga atttggattc cgccgactct cgcctcgatg attacgggac cgatagcaac
    24481 ctgcattttc aagcttgaaa tgaacggagc agcggtttct tcgggtatgg gcacatgcgg
    24541 acttgttggc cagataggcg tttacaccgg atgggtcaat gccattgccg acggcacaaa
    24601 ggcaagcatc accgcctttg actggatagg tcttgtcctc atcagtttcg tccttccggc
    24661 tgtcctctca tgggttttct gccttatcct tagaaaaata ggctggataa aggaaaacga
    24721 cctcaagctt gacctttaat aaagcaaaaa attcagcggt cgcaattgcg accgctgtta
    24781 ctttatacat atggatatat caagaaaagc ggccatcgtt tgatgaccgc cgttaattat
    24841 ttacagaatt ttggactcac accttttcat attactatga gttatgatta agtgtacatg
    24901 tcactcactc tcttctgagt aaattttgtt tagtttaaag ctttagtgaa acatcgtact
    24961 cactctttca atttattaac cggaagaagc tttacgtttt catcggactc actctctctg
    25021 aaaaaattta gttcaactta aactttaata aaacatctta actcactctc tcaatgttaa
    25081 tcgggaaaga tttaactgtt ctttggactc acgctttctt aatagatttt gatctgagtt
    25141 taagtcttaa agaaacattg cactcactct ctcaatttaa ttgatgaaga aaatcttacc
    25201 tatgcctttg gactcacgct ttctgttagt tatttggcat attgcctggt attgttatct
    25261 cttaacgaga ttcaattaac tgttcttcgg actcacgcct ttaataaaac atcttactca
    25321 ctctctcaag tttatttgat ttcaaaataa gattttatta aatatcttct tacttgatac
    25381 taagaaggat tttattacct gtacatttca actcactctt tcaatcttta tggaggggta
    25441 aattaactgt acttgatact cacctttcct tggattattc ggtgatttga gctttcatgc
    25501 tctccttcta tgtcatgtgg gcgttcccac tactccgcga tgccgggtgt ttaaaatctt
    25561 tactgtttat caattattca tttggctttg tcgtgccctc ttatttcaga tttaacagaa
    25621 ccggatggcg gccatattaa attgtgtttc atctatcgtg ttcattggac tcgcctccct
    25681 ttcttttatt tgggaaacct tggtttttgt tggtttattt tttgttgttt ccctttgttc
    25741 acctatatat tagcacgcgt tttatggttt gtcaatagtt tttttaaaaa atattgaaaa
    25801 tttttataaa atttgactta attgtatttt cgcttgacta ttaatcaaaa tgcggttaaa
    25861 atatatttat atgaaaacta cacggaggac gcaaaaatgg ctgaagaata caatccggac
    25921 atcgtcagcg tagttgacga gaacggcgaa gaacatattt ttgaagagct tgacagaatc
    25981 gaaaccgatg acgcacgtta cgccgccctt ctccccgtct atgacgacgc agaggatatt
    26041 cttgaggacg acggcgaaat aatccttctc aaggttgagg aagaggcaaa cggcgacaca
    26101 tatctttgcc cgattgaaaa cgcaaaggaa tttgacgagg ttcaggcaat attcgaggaa
    26161 agacttcagg atcttttcaa cgaagaagag gaataataag aagtaaaaac gcagccctgc
    26221 cttgtgcgat aacttacggt ccatattaac cctacaattt tatctaaggg agcccggctc
    26281 cgtgcacgga ttgcagacct cccggctttt gccggatgtt gggagcgaga actgtatggg
    26341 aggcacccac ctgctttaaa cgcaggttac tatcgaccgt atccggcttg gcggggttca
    26401 aaaacaaagg tgatttaatt gataagaatt gtcagagcaa acgtgcttga cattgttccg
    26461 ttggaagttg gctttatcta tgctcaaaag acacggcttt caaacggtca ggacacagtt
    26521 gctttttatg tatatgacca ggacggcgac agaacgctgc ctgttaaaat aaaaacttat
    26581 ctcgaggcaa aattcggatt tcagtaccgt gaaattgcaa aaaaactcgg taactacata
    26641 gcctgcgatt cagccgtgct caaaagcaac ggcgttgtta cactgtttga cgacggaaac
    26701 atgaatattt ttgactctaa cggcgacata gcaatgcagg aaaagctcgt ttatcagaac
    26761 tgccctatca gaagccttgc ccttgacgga acgtgtttct ggtgcgttgt gccggacaga
    26821 aattcaatcg taaaattctc ccctattgaa aagagagttc ttttgagaat cggcggcggt
    26881 gcatcggtcg cattcagacg tccgatctcc gttttcaaaa acaaggacaa gctttttgtt
    26941 tgcaatgagg caaccaatga gataagaaca ataagcctga aagattattc tgtaaacgac
    27001 tatatgaaat tcaaagagcc tctgaagaag tatttcaagg ttggtttttt tgaatatgtt
    27061 gtccttgatt cgggagttta ctgcctcgag gaaagggata aataatctga atacaaaacc
    27121 gtacatatat ggaaagaagc acctaaagaa aaggtgcttc ttttttattt ttgaggttat
    27181 cataatatag gtgatattat ggataaaatc aaagaatatt ttgttgtttt tgcctcggga
    27241 ggaataatct acagccttat tgaggttgtt ttccgcggtt ttactcactg gacaatgacg
    27301 ctgaccggcg gagctgcact gctgattatt tacatcacta acataaaaat caaaacgaaa
    27361 ggtcttatcg tccgctgtct tgcaggctgc gcgataatca catcgctcga attcattgtc
    27421 ggctgtattg tcaaccgagg gcttcacatg aaggtttggg attattccgg ggaaaaatac
    27481 aacgtgctcg gtcaaatatg tccgcttttc tctgtcctgt ggttcttact ctgtattccc
    27541 gctactctgc tctccttctt tttccgccga cgtttcggtg aatacagatg atttgatatt
    27601 tttgaattcc tcgctgaaat cgggatttgt gagcgaagta taagagaaaa atgccacgcc
    27661 gccgtagtta tccgttgcct ttgcgacgga tatttccttc tctattatgc cgtttcctgc
    27721 gtcaaactcc tcgtcgctgt cgtttatttt gtacgccgca attccgtata tcattctaac
    27781 ggcccctatc gtatccagct ttgaccaact gtcgcaggcc ttatcaaacg gtaccgcctc
    27841 gtcctcaaag ccgtaataaa gctgaggaat aatccaatca caatatccgc gctctgagca
    27901 ccagcgttca acgtcagcat actcctcctc atagttgttg ttgatatttc ctgccgggct
    27961 gatcgagaaa acgcagtcct tgttctcact cttgactgcc gtatacatct gagaaacgaa
    28021 cgaggaaatc atttcaagac gccatttgtc gtgaaacagc ttgccgccgc ttttcttata
    28081 atcgtcgtag taaaccttat ccacgctctc gtcggttgag ggatagaaat aatcgtcaat
    28141 atgtattccg tcaacatcat agtttttgat aatttcacgc acgccgtcga ttatcagctt
    28201 gtgattatcc gtcgaggcgg ggcaaagata cagaccgccg tccgcagtta cgacgcgttt
    28261 tttcgtcttt tcgtcgctca aaagaatttt tgccggattc tttttgctga gcttatcaag
    28321 atcggtatca aacgacaccc tgaacggatt tacccatgca tgaagtccga ttcccctctc
    28381 atgggcctct tcaaccataa tttcaagagg gtcatagtat atcggatctc cctgataccc
    28441 cgtcagatac gccgaccagg gaaatatatc cgacggatac attgcgtcgc aaaacggcct
    28501 gacctgcaca aacgcagtgt ttatgccaag ctttgcgcag ttatcaaaca tctctctgat
    28561 tttctctctg aacgatgctt cgtcaccgcc gtccgaatcc ttcatggaaa gttcgttata
    28621 tgatatccat acagcggaaa tctccttgcc gccgggcttt ttgctctttt gcgatttgtc
    28681 aggaattgtg caagaggtaa gcatcatcat tgcaagcaac aaacatatta tacgcttcat
    28741 attatttctc cgttgcggcg tatcggcctg ccaccaagcc gctcgcccat gcccattgaa
    28801 gattgaagcc tccgcaaatg ctgtccgtat cgagcacctc tccggcgcag aacagcccct
    28861 ttacaagctt tgactccatg gtttttctgt taaattcggt aacatccgca ccgccgacgc
    28921 ttatttgagc atttttgaat cccatagtgc ccgttaccgt gaaatcaagg ttctttgctg
    28981 tctttattat ttccgttatt tcttttttgc cgagttcacc gagcggagtg agactgttaa
    29041 tacccgctct tttgagtata taccttccga gtgccttcgg tatcataccc gaaagcgcgt
    29101 tttcaatcgg aattttctta tcaagcgaaa gtaaaaatct ttccgcttca tccgtttcgc
    29161 acctcggcaa ggtgtcaata aagcagttat actgtccgtt ctttacactt cggcttatat
    29221 ccattattga gattccggaa aggccgtaat cggtgaacag aacctctcct tttgcatcgt
    29281 caatcggctt aacgccttgt cggagactga catttgcctt ggctctcaca cccttgagag
    29341 attttgtgtt ttctttagtt gtgagctgaa caagtccggg ataaacctcg gttatgtgat
    29401 gaccgagcga tttgagcaaa ccatagccgc tgccgtcgga accctgcgac gcagaggcac
    29461 agccgcccgt gcaaatgata acctttttag caacaagctt tccgacggtg aaaatgccgt
    29521 ttttcttttc gattttttca acgtgactgt cgcatataag ctccacgccg agcctttccg
    29581 cctttgagcg gagagcgtca agcaccgacg aggcggtatt gctcatcggg tataccctgc
    29641 cttcgccgtc ggcaacgcac aaaacgccga ttgagcgaaa gaattcaatc accctttcag
    29701 gaggaaactc attcagtgca gccgaaacaa aatcgggatt tgtgtagctg tctgcagcag
    29761 cgttaaggtt tgaaagattg caccttccgt tgcccgtcgc aaggattttt ttgccgacct
    29821 ttggcatcgc atccaatgcg gcaatgttaa gcttacgatc ggtttgcttg gcgcttatcg
    29881 ccgctgcaag ccccgaagca cccgctccga tgattattat gtcgtaaatc atttcaccgt
    29941 ctccgtaaat tttatgattt attctatcat attttgcttg tcttatcaat atgattttat
    30001 cggagggacg caaaatgatt ttagatattt tttcagacac ggacagaata cacagttacg
    30061 ataaggatct cacgcaagca gccgaaaata taagaatcct acttcagggt acatatgcac
    30121 gttttaacga agcgaccgat ccgctgataa tcgaggcctt gatttacaga atgaaagaac
    30181 ttgaaactca gtacagcttt cttatcaaaa aagcgaaact tgaaagactc tgcggcgaga
    30241 tagactcaaa gggtgatttt gtatgaagta tgccttgatt gcaggagtat gcattattct
    30301 gctgatacct ataatcgcga tgttcaagaa aggcgctttt ttcaaatgca tttttctctc
    30361 ggcatttcaa ggcttggcag ccctgctcgc cgtgaactca ctcgggctgc tgacgggagt
    30421 aagtctcgcc gtgaattggt acacggttct tgcctgcgtc tgtctcggaa tccccgcaag
    30481 catctccatg ctggtgttag atattattat gtaaaaaaag cataggcctc tcatttcgga
    30541 aaatctacgc tgttatattt tattcttgtt ttatctgcat gagtgtataa tattgtttac
    30601 ggtctatctt aagttttcct atttaaacgt atcgagaatt tatttcactc tgtgtgcttt
    30661 acggatttct cacgtggatc ggtgtaggca tcgaccacta ctcttcactt ttagctttta
    30721 tcttatacga aacccatcag tcacagctca aggtcgtgtc agctccccta cagggacgcc
    30781 aagagaaatt cacgctgcct taacacaaca aaaagacccc gacatatgtc gaggtctgat
    30841 tatatttata tggacaatca gttgttgtcg cccttcttct tgatgatgtc gttaagaatt
    30901 acatagaaat cctcatcgtc aactccgctc tttgcctgct gcttggagct gttaacaacc
    30961 ggcggctcaa tcggagcgtt tgcagaagca gccgctgttg aacgaatcgg tgcacctgca
    31021 tttgatgttg acttaaaagt cgggatagag ccaaaatcct ctgacggctt agcgtcaaaa
    31081 ccggtagcga taaccgtaat gataatctca tcctcaagct tggagtcaaa tgcaacaccc
    31141 catgtgatgt ttgcatccgg atgtgccttc tctgtgatgg attcacaagc gttctcaatc
    31201 tcttcaagac cgatatccgg tgaagatgtg atgctgatga taactccctt tgcacctgcg
    31261 atagatgttt cgagcagcgg gcttgatgta gccatatcgg ctgcctgaag gctcttgtcc
    31321 ttgccctttg cagtgccgac gcccatgtga gcatagcctg cgtctctcat tacgctgcaa
    31381 acatcggcga agtcaaggtt aacgaagccc ggaaccttaa taagctccga aatgctctta
    31441 acgccctgtg aaagaacctc gtctgctacg aggaatgcct ctgaaagagt cttgcagcgg
    31501 tcgctgagga gcttaagtct gtcattcggg ataacgatga ggctgtcaac gttctctgaa
    31561 agacgtgcaa taccctgctc tgcgagcgtg cttctcttct tgccctcaaa cttaaagggc
    31621 tttgtaacaa caccgactgt gagaacgccc atttctttag cgatctcggc aattacggga
    31681 gctgcacctg tacctgtgcc gccgcccata cctgcggtga taaataccat gtctgttccc
    31741 ttgaggatcg aggaaatttc ttctctgctc tcctcggctg ccttctcgcc aacctcggga
    31801 cgtccgccgg cgccctgtcc tcttgnnnnn nnnnncaccc tgcataccct gagcgatcat
    31861 tgtgttgaca gcgtttccgc cgccgccacc gacgcctata accttgatct gaacggtgct
    31921 ctcgaaatcg ttatcaattt caaatgccat ttttaaaacc tccgttttgt aagtcatatt
    31981 tactccaagt tttacacatc tgttatgata tcatactttt ttatcaattt caagactaat
    32041 atcacgaaaa caggcaaaaa tatcggattt ttttcagttt tcacaagcta tgacctcata
    32101 catttatccg tcatgctttt tctgccttaa aaaagcggat tttcctcaat ttattcttcc
    32161 tcagccgtgt cctgtgccgt tgtattttct tcctccgact cggtttcctc ttccggaacg
    32221 gtcgtttctt cttcgccgtc cgtattgtct gcctctgtgg tttcgacctc gggctgcgtt
    32281 gttttttctt cagcggaaac aggttttgtt gttgcggcag cggttgtctt ttcgagcgtt
    32341 tcaagcggct ctacatagac ttttttcact atcgagagat tgatttcacc gatttgattc
    32401 gggttgttct cgtcctcctg tgcaattacc ttttttgcct ctttgagctt atactcaata
    32461 tcgctgtcat cgccgagata aagtctgtat ctgctctgat attcaagata gatattcttt
    32521 tgatcggaaa tgtcaatgcc cgttatgttc tttatatccg cactcttgat agcggcgcag
    32581 atattgtcat aaagcttttt ttcggtttcg tcggcaaagg ctataacctc tcccggcttg
    32641 gcatttattt tgttcttcac ggtcaaaagc gtaagtccgc tgtttttagg cttatctttt
    32701 atgatttcaa gtacttttcc gtttgagtcg gctatcgcat atccgttgac ggtcttaaaa
    32761 atcaaagccg ccttaccgtc ctttaccgtg aaagtaattt ttccgcttat gcttttgctt
    32821 atatcgagag aaaaaatata aggaagcttt tcctcagccc tcgctttaac cttttcccag
    32881 tcggttgtta tcatattgtc accgacctca acgcccgcgg catcaagtat ctgttcggcc
    32941 ttgtaatgag ttcctgcctt ggcgaccttt atttcactga cgttaaacag cacagtcacc
    33001 gaaagcacgg caaagactgc cgcaacggca acaaccgcaa taattcccgt aagaattctg
    33061 cggttacgag ttaccttttt cagttttttt tcgtaccgct ccctcgcctc cggtgaaagg
    33121 gcagcgacct gtcttgctgt cagttttttc attgtcagtc aatccttttt atttttgctc
    33181 ctatatagtc aaggctcttt tcgagcgctt catatcctct gtcgataaaa tgaatatttt
    33241 caacggttgt tcgtccctcg gcgcatagtc cggcaacggt cagcgccgca cctgctctca
    33301 gatcatgggc gcaaaccgat gctccctgca atcttccgac accgttgaca acggcaattt
    33361 tatcatggac gcttatatcc gcgcccattc tgttaagctc gcctgcatga cgaaagcggt
    33421 tttcaaatat attctcgcaa atcacggttg ttccgtcggc aacagccgcc atagccatta
    33481 taagagcctg acagtccgtc ggaaatcccg gatatggcat tgtccttatt gtgcccgttg
    33541 cgcaaagacg tctcggtgat attattcgca aacggttctg tgacgcggtt attttgcatc
    33601 cgctcagctc aaagcattgc attatcggca caatgtgacc gggtacaaca tcgtcgagca
    33661 gaatatccga acccgtcacc gctgccgcag ccatgtatgt gcaagctgca attctgtccg
    33721 gcataacggt gaactgcgta ccgtgcagac gttcgacgcc ctcaatatag accgtgctct
    33781 cccctgctcc gcttatccgt gcgccgcagg aattgagaaa cattgcaagg tcaactacct
    33841 ccggctctct ggcggcgttt attattgtcg tcgttcccga acagcgtaat gaagcgagca
    33901 taatattctc tgttgctccg acgctcggaa acgaaagtgc aatttttgct cccttcacct
    33961 tttgacattt gcacgagatg tgcccgaatt cctcggaaag actcacgccg agcttttcca
    34021 ttgcaaaaag atgcagatca atcggtctca ttccgatatc acaaccgccg ggagcgcaga
    34081 tatgagcctc gcccgtccgt gtgatcaatg cgccgagaaa aacaattgac gagcgcattt
    34141 cacgcataag ctcatcggga ataccgttca atttcattct gccgctgtct accgaaacgg
    34201 aatttccgct tcggtgcacc tcacagccga gtgattttaa aatgttcagt gttgagtcta
    34261 tatccgtaag cgacggacaa ttatgtatca cgcacggtct gccgacggca acggttgccg
    34321 caagaatcgg gagtgcgctg ttttttgcac cctgcacctt cagtctgccg ccgagcttcc
    34381 tgccgccctc aataataaag cgtcccatat atcgcaccct ttcggaaaat atcaatctgt
    34441 tctcagttta tgatactttc cgaaaagtgt taatttttta ctatcttaca aacgcactcc
    34501 gttatgcgct gcgaagcgtc aagtatagcc atggctcttg cgttttcgcc catcttttta
    34561 aggcgttcgg gctttgagag aagcttttca acctctgaaa gaagtctttc gcccgtgaag
    34621 tccttttcct caatcataat tgcggcatct ctgtcggaca ttgtttttgc attataatac
    34681 tgatgatttt ccgccgcata cggataagga acaagtattg acggcttgcc gagagcctgt
    34741 atttcgctca gtgagcttgc tccggcacgg cagacaacca gatcagccgc cgccagacaa
    34801 cgatccatat catttatata ttctctgagc attatctgcg aattagcatt aaggtcaacg
    34861 tccttgccga tgtcatcaat cattgaggcg cctgccttgc ccgtggcatg cagataataa
    34921 caatctttct tttcaaatct atggagtatg agatatttaa cagcttcgtt aattgccctt
    34981 gcgccgagac tgccgcccat tgaaagaata agcggacgtg aatcaagctt catttcggct
    35041 cttgcaaatt ccttgttcgc agagataatc tctcctctta tcgggagacc ggtaacgacg
    35101 cacggatttt tcggtttaag aagcttttct gcttcgggag cggtcagcat tacaacgtca
    35161 accttttttg cgagattttt gtttgtaacg ccggggaaag cgttctgctc gtgtacggcg
    35221 gtcggaatcc ccattctcgc cgcaacgctg agcacgggtc cgctgacata gcctccgaat
    35281 ccgataacaa cgtcgggctt gaagtcctca ataatctttt tcgcctgacc ctctgattta
    35341 aaaagcaaat ttaccgtttt tatattgtgc ttaagtcctt caaaattgag ctcacggctg
    35401 aagccctgaa tctcaatcgt tttaaaatca tatcctgcgg cgggaacaag ctgagcctcc
    35461 attctgtcgg cagtgccgac aaaaagtatc tccgccttgg gatagttttc tcttatgtag
    35521 cctgcaacgg caagcgccgg attgatgtga ccgcccgtgc cgcccgttgc gaataaaatc
    35581 ttttttccgt taagcatctg ttttcgccct cttttttctg tcgcccgacc ttgaaaccgc
    35641 aagcactatt cccatttcaa caagctgcat gacaagcgca gtaccgccat agctgaaaaa
    35701 cgggagtgaa atgcccgtgt tcggcataag atccgttaca accaggatat ttaatatagt
    35761 ctgcaagccg acctgaagtg aaattccgag aacgagcaga cgttcaaaca tgctctttgc
    35821 tcttgaagcg attacaaagc ccctgaaaac aagtgcggca aagagaagta tgatgagc
//
LOCUS       DAWBSW010000003        29212 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_328_length_29212_cov_3.127002, whole genome shotgun sequence.
ACCESSION   DAWBSW010000003 DAWBSW010000000
VERSION     DAWBSW010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 29212)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29212)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29212
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_29212_cov_3.127002"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15815..15824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21023..21032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7272 a         7268 c         6169 g         8483 t
ORIGIN      
        1 gtccagcgca aagtaaaaca aagtttgaaa agatggtaag aattctgtcg cccgcaatcg
       61 tagttgccga tgcagaaaaa atgctgacaa aaacagtcaa gccgagaacc actccgagcc
      121 cgacgatttt attcagagat tttttctttt gcgtgttaag atatcttatt actctgcgtg
      181 aatccttcgt ctgcgaatat tcgattccca caaagctttc cgattctttt cttctcggcc
      241 tttcctcgtt cgaggcaaac agctctccta ttttactgct ctccggatca agcccgtaag
      301 gatctccgtt ttcatccttt tcaagcaatg aaaacttgct gattttttct tttctctttt
      361 caaaaagctc tttctctgct tcgctctcgt cgattcggtt cggcgcttca tcctccgtgt
      421 ccattccgct gaaaaccatc tgtccgccaa gctcggcatc acgaatctta ttttgaaaat
      481 caccggcggg agtttgttct ccctccgctg ccttttcgtc ttcaagcgca tcgtccgccg
      541 acatgattat aggtgcgccc tcaagatcct cctgcgcctc acctttcttt ataacaaagc
      601 ccggcttttc gatatagacc tctcttgtcg cttcaagggt gggaatctgc cttgtttttt
      661 cgtcctcggg gaaagaaata ctttcaacat cttcctcctc tttttcctca agctcatcat
      721 caaaaacttc gtcgcttgtt tcttcctttt tctccgattt gtttttgaat gactcaaaga
      781 tttttttgcc ggaaattttt attttttttg cgaattcgga caggcttttc ttcgccgacg
      841 ctacggcttt ttcttctttg atcggcgcaa catccgtcat tattgatttc agcctgtcat
      901 caccgtctgt gttttcgtgc tcttcggaaa tatcaaaagg ctttatgttt tcttcctttt
      961 ccgtcttcat ttcgcccgac aaaaacttaa cggcattttc caggctttcg gcttcaaatt
     1021 catcgtcatc aatctcaccg tcaacgcttt gaccggactg ctcttcaatt acgatttccg
     1081 cttcttcggc atccttcgcc tgaacctcgt cgtgttcttc tgtttcttct acggtcggtt
     1141 ctgcttcgga ttcagagaat tcttcgttcg agacggactg ctcctcgggg actccgtctc
     1201 tctctcggtc aagctcctca agctgctttc ctctgtcggt cagctcaagc aaacgctcga
     1261 tatcctcatg cgaccagttt ttcgcatcgg tttctccgcc gtttttcttt tttatttctt
     1321 caatcagctc cataagctgt tcgtttgtta aatctgacaa ggatctcacc ccttagcttt
     1381 gattcccgac ctctgccttg cgacctcata catgagtaca cctgccgcaa cggaggcatt
     1441 gagtgaattt atttttcctt tcatcggcat agacacaatg aaatcgcatt tctctttaac
     1501 aagtcggctg ataccctttc cctccgaacc gatgacaagt ccgactccgc cgtcataatc
     1561 aacgccgcac cagttctctc cgtccatgtc tgcgccgtag aaccaaattc ctctttcctt
     1621 caactcatcc atagtctggg caagatttgc aaccctgaca acagggacat attctaccgc
     1681 tccggccgac gcttttccga caacatagct cagggaagcg tttcgccttt ttggaatgat
     1741 aactccgtga acgcccgtcg cctccgccgt tctgaggatt gctccgagat tatgcggatc
     1801 ctcaagctcg tcgcaaataa ttataaaagg cttttcgcct ctctcctctg caagtgcaaa
     1861 aacatcctcc atgcttgcat attcatgcga ggcggcataa gcggcaactc cctgatgagt
     1921 tgaagaattg cacatcatat caagcttttt tctgtcaacc tctttgatga cgactccaag
     1981 atcacggcat tttgcgagaa tctgtcctat tgagccgtta cgctcgccct ttgccacaag
     2041 caaagtatca atcgctctgc cgcttttgag ggcttcggaa acggcatttc ttcctattat
     2101 aagatcgtcg cgttcggtgc gtttttcatt atccataata atcctctatt ctgaaaatat
     2161 aaacatatat atattaacat atatcaggga caaaaaacaa gacaaaacgg cgtttatttt
     2221 caaacttcct caaatgttcc tctaatgccc ctttttcttt cgacctgttt ttccttaggc
     2281 gagttataaa atatagataa ccattgagta gcagggagat aagaattatg ttgaaaacaa
     2341 gagttaaaaa attcattacg gtaaaagagg acgagaaaca gcctaagctg caattcaaac
     2401 ccgttatgaa aacggtactg gcatttattt tcggattttt gctgatgaat ccttttgtta
     2461 cgggaacggt atcgcctttt tcaatatcgc tcattgcttc gctctcgggt atgcaatgca
     2521 ttgccgcaac agcaggaaca gtaatcggtt cttttgtttt ttttgatgcg accgacacgg
     2581 tgaaatatct cgccgtcgcc cttttttgct gtatgatcaa cgagatctgc gcacggtatt
     2641 tcggctcgga gctgatgaaa atcacgccct atataaattc atttctcagt atgctcatca
     2701 tcagcggagc aataatgttt gccacaggct ttgagcttga aacctttatt acgattatat
     2761 atgaatccat gctttgctgc gtcggcacct acattttcgt acaaggcaat ttacttgttt
     2821 tcggcgagaa ggatttttca cgcttcacga cgaaggaatt tatgatcact cttttatcgg
     2881 caggcttttt gctgatgccg ttttacaaat acaccgtgct gaatttttct tttgtaggcg
     2941 ttatcttttc ttttgtcatt cttctgctcg gcagactgaa aaacggaaac ggaggaaccc
     3001 ttgccggcgt ttgcgtcgga atatgtgtcg ggctcagcgg tcaggtttct ttcctcggtg
     3061 caggctatgc gctgtcggga cttttatgcg gcgagctttc acgcaaaaac aaatattttg
     3121 ccgctctcgg ctttgtcggc tgtgctgccg tttgtgcgct gattgattca tcgctcaagg
     3181 catatcttgc aattgttgag gctcttgttg ccgccgcaat atttttgctt ttgcccgaca
     3241 gatttttttc atttctcagc gaaaaagtaa atttacccgt tcccgtttac ataaaaagca
     3301 acaacagccg tgtgctgaca cgacgactga ccgaagcttc cgaagcgata accgagcttt
     3361 cggattgcgt tgacaccgtt cagagaactc tcgcccctca gcacgaaaaa gaactacggc
     3421 tcgccgtcaa gtcggcgtgg aacaaagttt gcgacaactg tgatttaaaa aagagctgcc
     3481 gtgaggaagt gcgctgcccg aacgacgaga cgatcgacaa aatctcaaga gctttgaccg
     3541 accgtgcaac tctcggcgaa atgcacttcc cgaaaaattt tcccgaagcg tgttactgct
     3601 ttgaagagat gaaagaaaaa atttccatga gatacatgag ctatgttgcg tcgctcggtg
     3661 cgcagggcaa ggttgagcaa atgcagggcc ttatgtccga ccaatttaag agcatggcgg
     3721 atattctgca tgatatcgcc tgcgaatttg acgaggattt aaatttaata taactcaatt
     3781 caccgatgcg ctctctatgg caacgggaac ggtacttgat attcccgata tcgacacaga
     3841 caacgatgaa tgtgtgatga aattcaagca ggaaataatt tacgatgttg aggtcggcgc
     3901 attcagccgc tccgccgatg atataaaggt ctgcggcgac tattacagga gctttcggga
     3961 cgaaaacggg aggcatataa cagtgctcag cgacggcatg ggaacgggca gccgtgcggc
     4021 ggtcgattcg gcaatggcgg cggagctttt ttcaaagctg ataaaatccg gactgagctt
     4081 tgactgcgcc ctgccgattg cgaactcggc gctgcttgta aaaagctcgg atgaatcgct
     4141 tgccactctc gatgttgttt gcattgacct ttatacgggc agaacggatt ttatgaaagc
     4201 cggcgcggca gctaccttta tccgccataa ggacagcgtc gcatctcttg agcaggcctc
     4261 gctgccgata ggaattctgc gtgaaattga gttttcaaaa gcaacggcaa agcccgagaa
     4321 cggcgatatt attttaatga tctccgacgg tgtgctcggt gactgcaacg gttggattca
     4381 gcaggaaatg aaactttggg acacaaaaca ttctccccgt gagctggcag aatttattgt
     4441 taactcggca tgtgagcgca agctcggtaa acagcgtgac gacatgaccg ccattgcaat
     4501 atatgttaag gaaaaaagat aaatagaaag gctgcacttc atcacaaagc gcagcttttt
     4561 cttttgtaaa attcatttta ttatatttct ttgagtcaca ttctatcttg ataacagcgc
     4621 cgtctttatc aatttttgca ctgcccgtaa aggcttcacc ctctccgctt tcatcggaaa
     4681 acatcacgaa caaatctccg ctgagtcctc ctcgtctgaa agaaacatga tagaccgttg
     4741 aaccgtcggt cattaaaaat tcttcgtctt tttcggaaaa gtcgagatat aaattgtttt
     4801 ctttgtcgta atatcttccg tcaaattttt cggcaacttg cagtccgtcg agcgaggaat
     4861 ttttttcggc acatcgaatt aaaattgctt ttccgccgaa cacaagcgca gcgaccaaag
     4921 cacaaatgca aacaattccg acgtacgctt ttttacttgt ctttttcatt ccgacggccc
     4981 ctttcagcca acatatccga cagtgaacgt atttgaaatt gagtaaccgt tcttattttc
     5041 gcaggtaaaa gggactgtaa ttctgtaagt gccgcttgac acgccgtcga gcgattctaa
     5101 atcaaaaagc acacaaaagc tcatcggaat tctgattgtt ttactttcat tcgttttgac
     5161 tgtgccttct gattcctgct taaatttttc ataaacctcc gcattgtaag aaagatcatt
     5221 ttcatcgacc gaataccaat tatcgcctat ctttttttca agtttaaatg acgaacagct
     5281 tatcggttta ttctcattat tttttatgaa taattcaaaa atcacagtgt cgtcttgata
     5341 atttgttccg ttcgttttta caattatttt ttcattgctt tcggatatgt catacgcact
     5401 ttttaaaagt tctgcggcag gcttttgtgt tttgtttttc tcaatatatg tcttgttgta
     5461 aatcctgaag cagtaaatat tttttatcaa gccgtaatcg gcaacgatgt tgtattcgct
     5521 tacttcactt gactgcaaat cggaaagtgt atataacctg ctcgccttat cgtaatcaac
     5581 tttgattcta tctccctttt catttgccgc caattcataa attctaagcg gcttgtgagg
     5641 aagcttaaat ttaatttttt cattttcatt aagcgcaacg acccgagtgt ttatgtccaa
     5701 caggtttgcc gttgcatcaa attcatcttg cattgtatta taaagataca ttttttcaaa
     5761 atagtattca atattttctg cttcgccgac aaaatcaatt ttttcttttg aaaagctgct
     5821 ctccacaccc aaagcaacgg caaatacgac aactagagca attatagcag caattatctt
     5881 tttcataata ttttcctttc ggaattttta tctataatca ttatacataa ttttttaccg
     5941 ttatcaacta aaatttgaat tatgtccgta tatttcttac ttaacgcaaa aaaacggcag
     6001 gcactttcgt gtctgccgtt gatttatacg gatttatacc tgattatttc gactcggtgt
     6061 caaccagctc gatgatgcac atttccgctg catcaccacg gcgggcgcct gtcttaataa
     6121 tgcggcaata accgccgttt acgtccttgt atttcggagc aatttcatca aaaagcttct
     6181 taacaacgtc ctctttggta acgtatgcta aaacctgtct tcttgaatga agtgtgttat
     6241 ccttagcaat ggtaatcatt ttctcagcca ttgcacgaac ttcctttgct ctcgtaacgg
     6301 tggtctctat tctgccgttc tctaaaaggt atgttaccat gcctctgagc attgacttgc
     6361 gatggtcact tgctcttcca agctttctgg ctcctggcat taaaagtcac tcctttaccg
     6421 ttagtgtgtt gtttgattta attacagctt tgacaatcag tcgtcatccc tgcgaagatc
     6481 gtatccgagg gaagcaagct ttgcaacaac ctcatcaagc gactttctgc caaggttacg
     6541 aaccttcatc atatcgtcct cggacttacc gatcaagtcc tccactgtat tgattcctgc
     6601 tctcttcaag cagttaaaag agcgcacaga caggtcaagt tcctcaatgg tcatctccaa
     6661 gactttttcc ttgccattct tactcttctc aaccattatc tcggtttcaa aagcctcatc
     6721 agagagatct ccgaaaagac tgagatgctc gttgaggatc ttggcgccca gagaaacggc
     6781 ttccttagcg gaaattgtac cgtttgtcca cacttcaagt gtcagcttat catagtcggt
     6841 tatctggccg acacgtgtgt tctcgactgt gtaatttacc tttaataccg gtgaatagat
     6901 cgagtcgatt gcaataacgc cgatcggcgg ctgaagatac tgcttgtttc tctcggcaga
     6961 atcatagcca cggccgttgt tacatgtcaa ctccattacc acatgagcgt cgctgtcaag
     7021 agatgcaatg tgaagatccg gattaaggat ctcaacatct gaatctgtct tgaaggagcc
     7081 tgcggtgatt tcaccttcac ctttaacatc aacgtacatg gtcttgggac catcgccgtg
     7141 aattttaagg attacgctct tcaagttaag aacgatttcc gtaacgtctt ccttgacgcc
     7201 cgggatcgtt gagaactcgt gaagaattcc gtcaatctta acggaagtta ttgcgtagcc
     7261 cggcaatgaa gaaagaagaa ctcgtctgag gctgttgccc agagttgtgc cgtaacctct
     7321 ctccaaaggt tctactacaa atttaccgta agtaccgtca ggtaaaacat cggctgtttc
     7381 gattcttggt ttttctatgc caatcattca aaaccctcct tgttgtatta agtatacggt
     7441 taattatttt gaataaagct cgacgatgag atgctcctca acggggtagt ctatatcgtc
     7501 acgcgacggc atagcaacaa ccttaccggt aaacggatca tctgtcttct caagccactt
     7561 cggaagaaga acaaccggtg ccttctcgcc tgtaagctct gtgaatacag agcttgatct
     7621 gctgttctcg caaacggaga taacctcacc cggcttaacc atgtatgacg gaatgtctac
     7681 acgcttaccg ttgatggtga aatgaccgtg agcaacgagc tgacgagcct gacgacgagt
     7741 tgctgcaaat ccgagacgga aaacaacatt gtcaagacgg cgttcgcaaa gaacaaggag
     7801 attttcacct gctttgccct gcatcttcgt agccttttca taatatgaat agaactgctt
     7861 ttcaagcata ccatatatga acttaacctt ctgcttctcg ttgagctgaa gagcatactc
     7921 ggacttcttt cttctcattt gacccttggg attacgatta gtttccttgt tcttgtaacc
     7981 cataacagcc ggagaaatgc cgagagcctt gcaacgctta gcaataggct gcatatttct
     8041 tgccatatca atatcctcct tcttctgaat tatacacgtc ttctcttcgg cggacggcag
     8101 ccgttgtgcg ggatcggagt aacgtccttg ataaggctta cctccaagcc tgctgtctgc
     8161 aatgctctga ttgcagcttc acgtcctgct cccggaccct taacgtaaac ctcaactgtc
     8221 ttcataccat gctcgatagc aatcttagcg gcagtctcgg cagcggtctg agctgcgaac
     8281 ggagttgact tctttgaacc tctgaagccc atctcaccag cgcttgccca tgatactgcg
     8341 ttgccctgtg tatcactgat ggtaacgata gtattgttga aggtggattg gatatgagct
     8401 gcgccgcgtt caatgttctt acgttcacgg cgtctgcgtg tcgcagtgcc cttcttagcg
     8461 gaacccttag gtgccatatt atccctccta attacttctt cttgttagca atagtcttct
     8521 tcggaccctt gcgtgtacgt gcgtttgtct ttgagcgctg tcctcttacg ggaaggccct
     8581 ttctgtggcg tacgccacga taacaaccga tttcgataag tctcttgata tcaaaagcgg
     8641 tctctctgcg gagatcaccc tcaaccttaa cgttgtggtc aatatactca cgcagttttg
     8701 ctacatcgtc ttctgacaga tccttaactc tgatgtcggg atctacgcct gtagctgcga
     8761 taatgtcgcc ggcagtttta cgaccgatgc catagatata agtaagagcg atttcaattc
     8821 tcttctctct cggcaagtca acacctgata tacgcgccat ttctcagttg cacctccata
     8881 aatggtttgc gtcggattag ccctgacgct gtttgtgctt aggattctca cagataacca
     8941 tgatttttcc cttgcgctta ataatcttgc acttctcgca aattttcttt acagaaggtc
     9001 tgactttcat ttgaatgccc tccttgaaaa attagcctta gcttattttg atcgccatgt
     9061 tattcttcct ttggtgaggt catatggcga aacttcgacc gtcaccttgt ctccgggaag
     9121 gatacgaatg taattcattc tgagcttacc ggaaatatgt gctaaaatct tgtgtccgtt
     9181 ttcgagctct acctgaaata ttgcgttcgg caatgcctca acaacaacac cttcaagctc
     9241 aagcatatcc tgttttgaca tatactgcac ctccttaaag cagagtgagt atcttcggac
     9301 cgctcggtgt aattgccacc gtgtgttcga agtgcgccga cagcttgccg tcacgggttt
     9361 ttactgtcca tccgtccggc aacgttttga cgtctgcctt accgaggttg atcatcggtt
     9421 caattgcgat cgtcattccc ggcaacaggc gaactcccct tccgggggtt ccgtaattcg
     9481 gaacactggg gtcctcatgg agcttgtgac cgactccgtg tccgacaaat tcacggacca
     9541 cggaatagcc tcttgactcg caatgatgct gtaccgcata gcctatatcg ccgattctgc
     9601 cgcctgcgac agccttttca atgcctaaaa acaggctctc cttggttgtg tcgcacagcc
     9661 gcttcgcttc ggagcttatc gctcctgccg cgaaagtagc agcattgtca ccgttaaagc
     9721 cttcgagaac cgcacccaag tctatgctta cgatgtcgcc ctcttcgata acacgcttct
     9781 tgctgggtat tccatgaata acctcgtcgt ttatggaaat gcatgcggtg gcaggatagc
     9841 cgttataatt aaggaagttt ggttttgcac cgcacttgat gatataatca taggctatct
     9901 tgtctatctc agccgtggta acccccggct cgacagcctc tcctgccaac tttaatgctc
     9961 ctgccgaaat gatgcaagct tctctcatga gagcgagctc tctgttcgtt ttgagcacta
    10021 tcatgcttaa tcctccaatg ccttgagtgt gagtgccgac gtgtcggcga tctcctcctg
    10081 gccttcaact attctgagaa ttcctttctt agaatagtaa tcttttagcg gctctgtctg
    10141 cttgtgataa acagcaagtc tgtcaagaac cgtttcggga gcgtcgtcct tacgctgaat
    10201 aagttcaccg tcgcaagcat cacatacacc ctcggccttc ggagccttat actccagatg
    10261 atacgaggaa ccgcaatcct tgcagacacg tctgcccgac attctggcag cgattttctc
    10321 atccggaacc tcgatatcaa tgactttgtc aatgattacg cccatagcgt cgagtgcttc
    10381 cgcctgagga atcgtgcgtg ggaaaccgtc aaggataaaa ccgtttgcac agtcgtcctt
    10441 agcaagtctt tccttgatga tgccgataac gacatcatcg ggaacaagct gacctgcatc
    10501 catgaaggac tttgccttga ggcccatctc cgtgccctgt gcgagggctt cacggattat
    10561 attgcccgtt gaaatcgcag gaatattgag agccttacag ataacctctg cctgtgtgcc
    10621 cttacctgcg cccggggcgc cgagaagaat taatttcata attaactcct ttttgattag
    10681 tcaagaaatc ccttgtaatg acgcatcatc atctgagact caagctgctt aactgtttca
    10741 atcgcaacac caacaaggat gataatcgaa gtaccgccaa gtgaaacgtt catcggctta
    10801 ccgcaagctg atgtgataag ctggaacaga agcgggaaga gcgcaacaac cgagagaaga
    10861 agagctccga gcaaggtgat tcttgaaagg atcttctgaa tataatcgga tgtcggctta
    10921 ccgggacgga tacccggaat agcgccgctg ttcttacgga tgttgttagc catctcgatc
    10981 gggttgtact gaattgaagc atagaaatat gcgaagaaga ttataagaag gaaatacaaa
    11041 atcacatata cccagcttga gcttgagaag aacttgaaga aatggtcata ccagccgtca
    11101 ccatccttgg caccaacgaa gaggttgatt gtcggaggaa gggagaggat tgaagaagcg
    11161 aagatgatcg gcataacacc tgacatatta accttaattg gaatatgtgt gctctgtccg
    11221 ccgtacatct tacggcctac aactctcttt gcgtactgaa cgggaattct tctttcggcg
    11281 ttatccatcc aaacgatgta agcgatcata agaagaagac aaatcgcaac aacaacagaa
    11341 aggactgcat aagcgccgcc gtatgagaag taaccgccgt ttgcatcgaa aagtgaagcg
    11401 atgattgacg ggattctcgc aacgataccg gcgaaaagaa taattgagat tccgttgccg
    11461 attcccttgt cattgatctg ctctccgagc cacatgatga gtgcacatcc tgcggagaag
    11521 cagaggataa tgacaagtgc ctggaagata tcgtcaaata cgccgatgtc tttcataaga
    11581 agaagataat tatttccacg aaggtagaaa taataagccg tactctgaag aagagcaaga
    11641 ccgaccgtta cataacgggt gattgttgca atcttcttgc gtccctcctc gccgtctttt
    11701 gaaagacgct caagagcggg aatagcaact gtcaacaact gaactataat ggagctgtta
    11761 atgtacggtg tgattgacat tgcgaaaatc gtaccgtagt taaaagcgtt acctgtcatc
    11821 atgttcaggt attccatgaa cgttccggac atctgtgcgt ctgagaaaat gccgttggca
    11881 cccgccgcga caaaaggaac cggaatggct gcaccgattc tgaagatcag aacgataaga
    11941 gccgtgaata aaattttctt acgaagatct gcgattttcc atgcgttggc aagcgtcctc
    12001 aacatttcaa atcacctctg caattccgcc cgcagcctcg atcttctgct tcgcagactc
    12061 tgagaatgcg ttagctttaa ctgtgagctt cttagtgagc tcgccgttac caaggatctt
    12121 tacgccgtca aaggaattct taacaagtcc tgctgctctg agagcctcag tgtcaacaac
    12181 tgcgccgttg tcaaacttct tctcaagatc cttaatgttg agagctacga tctctttgcg
    12241 gaatatatta ttgaaacctc tcttgggaag acgtctctgt aaaggcatct ggccgccttc
    12301 aaagcccgga cgcataccgc ggcctgctct ggctttctgg cccttgtgac ccttacctgc
    12361 agttttaccc tgaccggaac ctgcacctct accgatacgc ttgcgctcct ttgttgagcc
    12421 gggcgccggt gaaagttcgt gcaacttcat aattcgcacc tccttgctta tgcttcggtt
    12481 acagcgataa gatgctgaat tttcttcacc ttaccgagtg tctgcggatt gttcggctga
    12541 accgaaacat ctccgatctt atgaagacca agtgcatgag cggtggcaat ctggtccttt
    12601 ttgctgccga ttaagctctt aacgagcttt acctgtacat ctgccatttg taccgccctc
    12661 cttaacctaa aatttcctta gctgacttgt cgcgaactgc tgcaacctcg tcaacgtcac
    12721 ggagtcttga aagtccgtca atagtagctc tgactacatt gcacggattg tttgaacgca
    12781 gtgcctttga acggatgtct ctgattccga cggactcaac gacagcacga accgggccgc
    12841 ctgcgataac accggtaccg ggagcagccg gcttgagaag tacaacgcct gcgccgtatt
    12901 taccgatgat ctcatgagga atagttgttc ctctgagaga aactttaata aggttcttct
    12961 tcgcatcctc gataccctta cggatagcgt ccggaacttc gcccgacttg ccgataccga
    13021 agcctactgt tccgttgccg tcgccgacaa ctacaagagc agcgaacttg aagatacgac
    13081 cacctttaac tgtcttagaa acacggttga tagtaacaac gcgttccgta agttccatag
    13141 ccgatgcatc aatcttagcc aaaagtaatt cctccttacc ttagaacttg aggccgccct
    13201 cacgggcgcc ttcggccagt tcctgaacac ggccgtgata tatatagcca ccgcggtcaa
    13261 atacgcattc ttcaatgcct ttgtccttag cgcgctgtgc gagtacctcg cccaccttgt
    13321 gagctgcggc tttgtttccg ccgtattccg tgaaatcctt ctcaacagta gaagcgctga
    13381 caagtgttac gcctgcctcg tcatctatta actgagcgta gatgttctgt aatgagcgga
    13441 atacatcaag gcgggggcac tgagctgtgc cggagatctt gttacgtact ctctggtgac
    13501 gtgcaagtct cgccttatta ctgtctggtc tgttaaccat aagtttttca ctccttcatt
    13561 aagattattt gccaccctta ccggctttac cttccttacg gcggatatgc tcatcaacat
    13621 acttgatgcc cttaccctta taaggctccg gaggacgctt ctcgcgtact tcagcggcaa
    13681 actgacctac cttctgcttg tcaggaccgg aaataacgat tttgttcggt gagggaactt
    13741 cgatcttgat gccctcgggc tgatccatga atacctgatg tgaaaatccg atattcatta
    13801 cgagctgtgt tccctgcata gcggcacgat aaccgatacc gttgatctca agctccttct
    13861 tgtatccgtg agcaacaccc tcaaccatgt tggcgatgag tgttctcgta agaccgtgaa
    13921 gagatctgtt gagcttttca tcattcggac gagtaacaac gatctcgttg ttctcaactg
    13981 caacagcgat attgctgtgc actgttctgg taagagttcc ctgaggtccc tttacggtaa
    14041 cagtgctgcc gtcgattgta acatcaacgc ctgcaggaat tgcgattggc aacttaccta
    14101 tacgtgacat tctaacagca cctcccctta ccaaacgaat gcaagaactt caccgccgac
    14161 gttagccttg cgtgcgtcct tgtctgtcat aacgcccttg gatgtggaaa cgattgcgat
    14221 accgagaccc ttcatgacct tcggcatgtc ctcacaacct gagtaaattc tcagaccggg
    14281 cttagaaact ctgcgtaaac ctgtgatggc tgcggtcttg ttctgaccgt acttgagggc
    14341 gatctcgatc atgccctgct ttccgtcatc ctcaaccttg aagcctttga tataaccttc
    14401 atcaacaaga atctgagcaa ttgccttctt catgttagat gccgggattt tgacagaatc
    14461 atgttttgca gaacttgcgt tgcgaattct ggtcaacata tctgcgattg aatctgtagt
    14521 ttgcataccg tcaacctcct taatgcaatt actctaaatt accaacttgc tttcttaact
    14581 ccggggatct gacctgcatg agcaagctcc ctgaagcaga tacggcaaac gccgtactta
    14641 cgaagatatg catgtggtct gccgcagatc ttgcatctgt tatatgctct tgatgaatac
    14701 tttgcagtct tggcctgctt aaccttcatt gatgtcttag ccacaattat acctccttat
    14761 ttctcgaacg gagcacccat aagcgtaagc agctcacgag cctcttcgtc tgtttttgct
    14821 gtggttacga aacaaatatc cataccacga accttatcta ccttatcata atcgatttcg
    14881 gggaagataa gctgctcttt gatacccatg gaataatttc cgcggccgtc aaaagagtta
    14941 gcgttgattc ctctgaagtc acgaacacgg ggaagagcga gattgaagaa tctgtcaagg
    15001 aattcataca ttctgcttcc tctgagtgtg accttaacgc cgattgtcat gccttcacgg
    15061 agcttgaagt tagcaactga cttcttagcc ttgcaaagaa caggcttttg gcctgtgatc
    15121 tggctgagat cgcttacgat agcgtcaaca accttcgggt tgtctcttgc ttcgccgcaa
    15181 gcaacgttga taacgatctt atcaagctta gggatctgca tgacactctt gtattcaaac
    15241 tttttcatca aagccggtgc gacttcgtta acataggttt cttttaacct tgctgccatt
    15301 tgtcatgtac ctcccttatt aaaattccgc gttgcacttt ttgcaaatgc gcttcttatc
    15361 tttcttgccg tcagccttag ctgcgtgacc gactcttgtc ggtctgccgc acttcgggca
    15421 aacgagctgt accttatcgg catagagtgc gctctcagcc tttacaaggc ctccctgctc
    15481 acccatctgt ctcggcttaa catgctttgt tacgatgtta atgccctcaa cgataacctt
    15541 accctctgaa ggagcaacct ggagaacctt gcctttagct ccacggtact taccgttgat
    15601 tacgatgacc tgatcaccgg tcttaacgtg tactttatta ctcatagttg caccccctga
    15661 ttaaagcact tcgggagcga gtgaaaggat cttcatgaaa tcatggtcac gaagctctct
    15721 tgcaacaggt ccaaaaatac gtgtgccctt tgggttttta tcgtctctga tgatgacagc
    15781 agcattctca tcaaaacgaa tatatgtgcc gtcgnnnnnn nnnnttcatt ttccatcttc
    15841 atagcttcca gaatcaatac aacctcgccc ttctttacga cgccgccggg tgttgctttc
    15901 ttaaccgaag caacaataac gtcgccgatg ttggcgtatc ttctgccggt accaccgaga
    15961 actcggatac atctgatctc cttcgctccg gtgttgtcgg caactttgag gttactttct
    16021 tgctgtatca cagtgtttgc ctccttacac ctgcgccaat tatttggctt tctcgataat
    16081 ctcgactaca cgccatctct tgtcctttga aagaggacgt gtttccataa tcaatacctt
    16141 gtcgcctacg ccgcactcgt tcttctcgtc atgagccttg aacttgattg tacggttaac
    16201 gatcttctta taaagcggat gctttatctt gtccttaaca gtaacaacga ccgtcttgtc
    16261 catcttgtcg ctgctgacaa cgcctacacg cgtctttctg aggtttcttt cttccattac
    16321 agttaacctc cctttcatta cgactcagcg ccgtgaagtt cgatatcacg ctgaacagtc
    16381 tttattcttg cgatatcctt tttaacggcg ttaatacgca ttgggttgtc gagctggtta
    16441 atggcctgct ggaaacgaag cttgaaaagc tcatccttaa gatcgtgaag cttggtgtca
    16501 agctcgcttg cggacatttt tctgatttca cttgctttca ttactgctca ccacccgttt
    16561 cttctttagt aacaaacttg catttgatcg ggagcttgtt agctgcgaga cgaagagcct
    16621 cacgagctgt gtcctcatca acacctgcaa tttcaaacat aactctgccc ggcttaacga
    16681 ctgcaaccca gtattcgggt gatcctttac ctgaacccat tcgggtctca gccggcttct
    16741 ctgtaatcgg cttatccggg aaaatcttga tccaaacctg accgttacgc ttgatgtaac
    16801 gagtcatagc gatacgagct gcctcgatct ggtttgatgt gatccatgcc ggctcgagag
    16861 cctgaagtcc atattcaccg ttggtaacct tgttaccgcg agttgcctta cccttcagac
    16921 gacctctctg aacacgacga tatttaactc tcttaggtaa cagcattatt tagctccctc
    16981 cttacggcgt ggtctgcgat gattgttgtt agcctcatgg agaacctcgc ccttgtaaag
    17041 ccaaacctta acgccgatac gaccgtaagt tgtctttgcc tcagcaaagc cgtagtcgat
    17101 gtcggcacgg attgtctgaa ggggaattgt accctcatga taagtctccg aacgtgcaat
    17161 ttctgcgccg ccgagacgac ctgaacactg aattttgata ccctttgcgc cgagcttcat
    17221 tgtgcgtccg atgcactgct tcatagcacg gcggaaggaa atacgtcttt cgagctgaga
    17281 tgcgatgttc tcagcaacaa gctgtgcgtc aaggtcaggc tgcttaatct cgataatgtt
    17341 gattgaaaca tccttgtctc cgccaagctt cttcttacaa atttccttga gcttctcgat
    17401 ctccgagccg ccgcggccga ttaccatgcc gggctttgcg caatgaatgt tgatataaac
    17461 acgctttgcg tctctctcga tttcaacctt aggtacacct gcaggagcga gtgtatcgag
    17521 aaggtattca cgaagattat aatcctcaac aagagtatcg ccgaaagtgc tcttaccggc
    17581 ataccagcgg gattcccaat tcttaataac accgattcta agaccggtag gatttatttt
    17641 ctggcccata tgtttacctc ctcctcgctt attctgcttc tctgagtact aatgtaatgt
    17701 gagaagtctt cttgtttatt ctgtatgcac gtccctgtgc acggggtctg attctcttaa
    17761 gagtcgggcc ctggcctacg aagcactctg caacgtagag gctggaaacg tccatatctt
    17821 tgttttccgc atttgccata gctgacttaa gcagcttttc aagcggttca cacgcagcct
    17881 tcggcgtgtg tttaaggata gccattgcct ggtcgcaagg cttatttctg ataaggtcaa
    17941 gcacaatctg aacctttctc ggtgcaatac gcacgtaggt tacttttgct gttgcttcca
    18001 tgaatacaca ctcctttcgg ctaattattt accattattt gttgtttttg aacctgcatg
    18061 acctctgaag gttcttgtag gtgcaaattc gcccaactta tgaccaacca tgtcctccgt
    18121 aacatatacg ggaacgtgtt tgcgtccgtc atgaaccgca aatgtgtggc cgacaaagtc
    18181 agggaagatt gtggagctgc ggctccatgt cttgagaact attttttcgc ccttctcgtt
    18241 catggcctgt actctctgga acaacttagc ctgcacgtag gggcctttct ttacgcttct
    18301 gcccatagat ctttatctcc tttcaactta tttgccgtta cgacgtctta cgatcatctt
    18361 gttggaagcc ttcttcttgt tacgagtctt gtaaccgaga gccggcttgc cccaaggtgt
    18421 aacagggccg ggacgaccga taggtgactt accttcacca ccgccgtggg ggtgatcgtt
    18481 cgggttcatt acagaaccgc ggactgtagg tctccaaccc atatgacgtt ttctacctgc
    18541 gttaccgatc ttaacgttct cgtgatcaag gtttccaacc tggcctactg ttgccatgca
    18601 gtttgcggga accttacgaa gctcgcccga gggaagtctg aggagagcat attcgccttc
    18661 cttagccata agctgtgcag cgttacctgc agctcttgca agctgaccgc cgcggcccgg
    18721 ataaagctca acgttgtgaa taacggtacc tgtggggata ttaacgagcg gcagtgcatt
    18781 gcccggcttg atatcggctt cgggacctgc cataaccttg tcgccgacct taaggcctgc
    18841 gggagcaagg atgtagctct ttacgccgtc ctcatacttg atgagagcga tgtgagctga
    18901 acggttcggg tcatattcga gtgaaactac ctcagcagga acatcgaact tggaacgctt
    18961 gaagtcgatt acacggtact ttctgcggtt tgcgccgccg cgatgacgta ctgtgatgcg
    19021 accgtaattg ttacgacccg atttattctt gagcggctca aggaggcttc tttcgggagc
    19081 aacctttgaa agctctgaat aatctgtaac cgacatattt ctacgtgaat ttgaagtcgg
    19141 cttatagact ttgatcgaca tttgattcac actcctttaa gtggcttcgc ggagtatgtc
    19201 gaactccata cattaccacc ggaattttac ggtttgtttt gtgatgtcag attacatcat
    19261 gccatcgaag aactcgatgg tctttgaatc ctctgtaaga gtaacgatcg cctttttcca
    19321 agcggctgta tagccctggg aaacgccctg tctgcgaagc ttgccgaggc agttgatggt
    19381 gttgacctta gctaccttta cgccgaaaag ctcctcaacg gctgctgcga tctcaatctt
    19441 gtttgcatcc ttggcaaccg agaaggtgta cttcttatcg gctgttccga gcatgctctc
    19501 ttcagtgata atcggacgga ggattatgtc ctgtgctgct ttactcatgc atatacctcc
    19561 tcaatctttg caacgctgtc cttaagaacg agcattgtgt cacaattaag aatgtcataa
    19621 acattcaatg tgtttacggt tgaaacctta acaccggcaa tgtttcttgc gcttctgtaa
    19681 agaacatcgt tgttctccgg aagaacgaga agaaccttct tgcctgtttc aagagctgta
    19741 agaaccttta caacttcctt agtcttgatt gcgtcaagct tgagatcgtc aagaactaca
    19801 agctcctctg ctgcaacctt tgaagagaaa gcagacttca tagcaagtct tcttaccttc
    19861 ttgttgattg agaagccgta ctctctcggc ttggggccgt gtgcgatacc gccgtgtgtc
    19921 cactgaggag ctcttgttga accctgacgt gcacgacctg tacccttctg cttccacggc
    19981 ttcttgccgc cgccgcttac ctctgagcgt gtacgggttg actgtgtgcc ctgacgctga
    20041 tttgccaaat agttaactac gcaaagatgc atagcataag aattcggctc gattccgaaa
    20101 acagactctg caagagcaag atcggagacc tttttaccgt tcatatcgaa tactgataca
    20161 ttaggcatac tttaacactc cttccgttac gctttcttgg catcggcgat aacaacgatt
    20221 ccgcccttcg ggcccggaat tgcgccctta acggcgatga ggttgttctc gacgtcaacc
    20281 ttagcaatag ttaaattctg aactgtggtg cggtctacac cgtagtgacc tgcaagcttc
    20341 tttcccttgt aaacacggga aggagttgaa caagcaccca atgaaccggc atgtcttgca
    20401 acgggacctg taccgtggga ctctctgagt cttgagaagt tccagcgctt gatggaacct
    20461 gctgttccgt gacccttgct tgtgccgata gcgtcaacct tgtcgcctgt tgcgaacaca
    20521 tcggccttga ggatatcgcc tacgttaacg tctgcaatgc tctcgagacg gaactccttg
    20581 agtaccttct tgggagcaac atcgttcttt gcgaagtgac ccttcatagc cttgttgact
    20641 ctctgtgcct ttacgtcacc gaagccgacc tgaacagcct catagccgtc attttcgatc
    20701 gtcttcttct gtgtgattac acacggacct gcttcaacga ctgttacggg gattacgtta
    20761 cccttttcgt cgaagatctg cgtcataccg attttctttc cgataagacc ctttttcata
    20821 taagttttcc tccttatagg ttattggttg gcaactgcgc caacttgacc tgttagcgta
    20881 aaatcaaagc ttgatttcga tctctacgcc ggcaggaagc tcaagacctc tcaaagcgtc
    20941 aacagtcttg gctgacggtc tgatgatgtc gataagcctt ttgtgtgtgc gctgttcgaa
    21001 ctgctctctg gagtccttgt acnnnnnnnn nnggtcatag cccttcaatc tgattctgat
    21061 ttttcccttt actgccatga ttttgcctcc ttacaaagtt tggcgggcaa cgtttaaaac
    21121 ctaatttcgc aacgtttccg ggtgtttcca ccctctgcat ttaaaaaccg gaaatgggtt
    21181 agtttccggg atagtgcatt taaggtctta aacgcacata cacgcgcaag tttgctaagg
    21241 gatagcaaac ctgtgctgta ggttattaaa tcgcccggtt ttaaatcgga catactccgt
    21301 gagaattccc gacttctacg agccgccacc tctcacatca acgcatatct acgcactaaa
    21361 ctacataata atatagcatg ctcgagtatc ataccacact ttatttcaaa ttgcaagctt
    21421 tttttgaata tttttaaaaa attttttgag caaaaattac ccccgaaagc aagctgtatc
    21481 aaggctttcg gggattttta acttgaattt tccacacttt cccacatagt cggttctgca
    21541 caaaaaccgt acccatatat tgtggtttat gacggttgac aagagagcaa aaaaatcaga
    21601 ttttatcacc gaatgaatat catatacatt tatatatata atatataatg gcatcgtctt
    21661 ttttgcaaaa aaatgataat ttgcgctcac tgcccctatt tcaaaaacgg agactgaata
    21721 aacagcctcc gttttagtta tcttttattt aacaacgatg tttacaagtc tgcctttgac
    21781 atatagctcc ttgacaacgt tcttgccctc gattgcggcg gcgattgccg gatccttctt
    21841 ggcaagctcg attgcctccg ccgagtcaat ttctgccgga atcatgagct ttgcacgaac
    21901 cttgccgttg atctgtgcaa cgatttcaat ttcatcgtca atgcactttg cttcatcaaa
    21961 atcgacccag tgaccgtcag ccagcattcc gccgtagttc tgagccgccc aaatttcctc
    22021 cgtaacatga ggtgcgaacg gattgagaag cgttacaaac gttttcagct cggcattgtt
    22081 gattgagcct ttattataga tatcattgag caatgtcata agtgcggcga tcgccgtatt
    22141 aaacttcatg ccctcgatat cctcggaaac cttcttgatc gtcttgtgga aagctgattc
    22201 aagctccttt gaatacccgt caccctctgt gaggatatcc tgaagtgccc acactctgtc
    22261 gataaacctc ttacagccct taaccgatga ctttgaccac ggagcagctt tctcatagtc
    22321 gcccatgaaa agaacatagg tacgaagaac atctgcgccg tactcatcaa caacatcgtt
    22381 cgggtcaact acgttgccct tggacttgct catcttgtcg ccgtcgggtc cgagaataag
    22441 accctgagcg gtacgctttg catacggctc atcatacgga acctcgccga tatcatagag
    22501 gaacttatgc cagaagcgtg aataaatcat gtgacgtgta acatgctcca ttccgccgtt
    22561 ataccagtca acctcgcccc aatacttgag cttatccttg tcggcaaaag ccttgtcgtt
    22621 gtgaggatca atgtatctca ggaaatacca tgaagatcct gcccactgag gcattgtgtc
    22681 ggtctcacgc tttgcgtctg cgccgcactt cggacacttg cagttgacaa aagaatcaat
    22741 ctttgcaagc gggctttcgc cgtccgaacc cggctcaaag ttttcaacct tcggcagctt
    22801 gagcggaagc tcttcataag gaacgggaac catgccgcaa tgcgggcagt aaacgatcgg
    22861 aatcggctcg ccccaataac gctgacggtt gaatgcccag tccttcatct tgtactgaac
    22921 acccttttcg ccgatgccct tttcgctgag ataatcggcc atcttctcaa tagcttcttt
    22981 tttattctta attccgtcga ggaagcccga attcacgcac tcgccctcgc cggtatatgc
    23041 ttcttttgaa atatctccgc ccttgataac ctcgatgata tcaatgccga acttcttggc
    23101 aaactcatag tcacgggtat catgtgccgg aacagccata attgcgcctg taccgtagcc
    23161 catcataaca tagtcggaaa tgtaaatcgg aatctccttg ccgttaacgg ggttgattcc
    23221 cgaaagaccg tctatcttaa cgcctgtctt ttccttgaca agctgagttc tctcaaactc
    23281 tgttttcttt gcgcattctt ttctgtagcc tgtaatctcg tccatattgg caatgcgatc
    23341 ggcatactta tcaatcatcg gatgctcggg agccataacc atgaatgtaa cgccgaaaag
    23401 agtgtcggga cgggtcgtat aaatcttgag cttttcgtta tccgagccct tgacgtcgaa
    23461 atcaacaaaa gcgcccgttg acttgccgat ccagtttctc tgctgaagct tgatattcgg
    23521 aagatagtca acctcatcca gtccctcaag gagcttgtcg gcatactttg ttatgcgaag
    23581 ataccaaaca tcctttgact tctgtacaat atcactgtgg caaatatcgc acttgccgcc
    23641 ctgcgaatcc tcgttggaaa gaacaacctt gcaagagggg cagtagttaa caagcgtttt
    23701 atctctgaag gcaagtccgt gctcaaacat cttgaggaat atccactggg tccacttgta
    23761 gtatccctcc tgggtggtgt cgattacacg agtccaatca aaggagaagc ctactttttt
    23821 tagctggctt gaaaatcttt cgatattcat gtccgtaacc ttacggggat gaatacctgt
    23881 ttttatggca tagttttcgg tcggaagtcc gtatgcgtca aagccgatcg gaaacagaac
    23941 attgtagccc tgcattcttc tttttctgga aatgacctca aggccggaat aagccttaat
    24001 gtgtccgacg tgcatacccg ctccgctggg ataagggaac tcaacaaggc cgtaaaactt
    24061 cggtttgtcc gaaaaatcca ccgcatggaa aacgcctgtt tcctcccatt tatcctgcca
    24121 ctttttttct accgatttaa aatcataacc catttcaata atcctccgat gtgtattgcg
    24181 atttgctgca taggtatgct tgcaaactta ctctgaaata atgcaagttt tatgctaagc
    24241 ctccccttgt tgcaagggta agagggtgac cgcctgcggc gttttaaccc ctcagtcacg
    24301 gacgaaccgt gacagctccc ctacaggggc gcccaagaca agcacctatg caaaataaat
    24361 aatcagtcct cggtcagaac gtcggaaagg tcaatccttg ccgcatcgtt ccaaagacgc
    24421 tcgatgttgt aatactgtct gctttccggc tggaaaacat gaacaagaac atcgttataa
    24481 tcaagcagta tccagcccgt cgctctgccc tcgatgtggt caacctcaac gccgagcttt
    24541 ttcatctcgt cgtcaacctc atcggcaagc gcacgaacat gagtgtttga tgtaccgctt
    24601 gcaatgatga aatagtctga cattaccgtt agctcttttg tctcgattcc gatgatgtct
    24661 atcgcctttt tcttgtccaa aatcttggca atgttttttg ctttttcgag tgatgtcata
    24721 aactgtttcc tccctgcttt ttaataataa tttcattgta aaggtcaatg ctgtccggat
    24781 gaataacctg accctttgaa acaaggtcgg gaattccgaa aaccagcgca tattcgatcg
    24841 ccgaatccat gtccttttcg caaagccttc gcatatcctc aacgccgtta taatttctct
    24901 cagcagaaat atagtcggcg agataaacaa ttttttcgag cagactcatt tccgctctac
    24961 ccgtcgtgtg atatctgacc gctctgatta tttcacggtc gtctatgccc ataacgaact
    25021 ttatgtaggc tgcgcccgcc atagcgtgcc acagcttttt gttggctttt tcaacaggca
    25081 tcagctcaat gcccgcctcg ctcaataccc ctaactgctc ctcctcggaa gcatttttca
    25141 tcacgtcatg cagaagcccc gccgtatatg ccttatccgc atcaccgccg tatctgagag
    25201 caagctctct tgcgctgtcg gcaacattga ggctgtggtt aaatctgtaa tcatcaagtc
    25261 gggacttgat aagtgtgacg attttatcat ctgtccaatt gctcaaagag gatatctcct
    25321 ttatcaaaat tatctgatat ttaccttctt gttcataaca tagaacgttg catagtaaag
    25381 acctacgccg aaaataaggt aagctataac cgaaacaagg tcggtaaagc ttgcgccgga
    25441 tgcaagctga tcatagcttg ttataataga aagatcgttg ccgacagtcg caatgccgaa
    25501 aacaatgtta tcggaaataa ttcccgatat tctttcaacg gcaaaaagga tcacaatcgt
    25561 gaaaactatc gtccaaagaa ggtatttctt ctggaagtga cgtgtgtttg caagagaaat
    25621 tgcaaaatac atttcaaggg tgataactgc gaaacgcaga aaatatgaaa ccattgaaat
    25681 cacaatagaa gtgataatag tcgaaacgga tttaccctga aggaacattg gcagaagcga
    25741 ttcaatcgtt gagtatgtct cctcgccgat aacgtcttct ttaacaacaa aaaacgttat
    25801 agccgctgtg cccataaggc aaacaaaagc tgcaataaac catacggttg atgtgatgat
    25861 cttagaaata aggagcgttg agcctttaac gggaagcgta aagctcaggt aaccctggtc
    25921 gccgtaaagc gacttttgga aattcattat cataacaaca gccgtgagaa taaaactgca
    25981 tgatgaaatg agaagaagca ccataatgcc aagcatttga cctgtcgaag cggtatcctt
    26041 ggtaatgtaa taggagccga ggacatatgc catgattacg gcgacgactg cgtagagtgc
    26101 gcccatgatt ctgcccgtgg caatgaattc atttttcaaa agcttaccca acatagctga
    26161 acacctccct gaacagatcc tcaaccgttt tgccgtcagc cgctatcttt gaaacgtctg
    26221 tgttgacgac aagcttgccg tccttgacca tgagcacacg gtcaagcacc ttttcgatat
    26281 cataaataag gtgggtcgaa agcaaaatcg ttgaattttc ggcgtggttt ttcataatgg
    26341 tgtcaagaat aaattctctt gaaacaaggt caacgccgcc gaggggctca tcgagaatat
    26401 aaagatccgc ctttcggctc ataacaagaa gaagctggag cttttcctgc tgaccctttg
    26461 acatagtctt aattttctgc ttcatcggaa gctcaaacct tgccgccata tcaagcgctt
    26521 tttgcatatc gaaattgtca tagaaatcag ccatgtactt aattgcgctt ttcggcgtca
    26581 tccagttggg aagatagttc ttctcgggca gatatgcaat aatttttttc gactcaacgc
    26641 cgggcgcttt tccgtttatg aggatcttgc cctcatagtc attgatcagt ccgacaatgg
    26701 actttatcat cgaggttttg ccgcagccgt tcgggccgag caagcctacg attttgccct
    26761 gttcgatgtc gtaagagacg cccttgagaa cggcatgatc gccgtagctc tttttgaggt
    26821 ccctcacttt cagaagtgtt tcactcattg atgtattcct ccaatcccat tcatgggaaa
    26881 atcagtcatc agccaatctg gcttcctcaa taatatcctc tgtgatctcc tccgactcgc
    26941 catgcttaac gaaaaggata cagatcaagg atagaataaa gcatatcggg cagtagtaga
    27001 acagcgagcc gtatgtaccc gtgagcatac gaacgattcc gtaaacgatc ggggttgcta
    27061 tctgcgccga gaatgttgct gtataataat aaccagtgaa tgtgcctacc ttgtcaagtc
    27121 cgcctatttc aagaacaagc ggaagcgtat taacggtgat aagcatattt gcgaatccgc
    27181 cgacaacaag cgcaacatat acaagagtca aggcgttgcc tgttgttgca tcaactacaa
    27241 gataaaccac aaatgcggtt atgaacataa gaagtcctgc gacaattgtc ttttttctgc
    27301 cgaattttct tccaagaaga cctgcaggaa tagcgccgat tgcagttgaa accgcaaaaa
    27361 tcgtaagcat tgacgttgcc tcgccgcttg acttgccgag gatctcggca gcaaacagtg
    27421 cgaagaaggt ctggatcgcg tttgctccgc agaaaaggaa gaaaagacct gcaagcatac
    27481 agataaggct gactctttcg cccttggaaa gcttgattgc cttgagtctc tccttttctt
    27541 tcttcgccgc ctctttttct gcctggcgct ttgcctttgc gtcattttcc atgtttgcct
    27601 gcatctgaac cttcagtctg ctgtcaggct ccttaacgaa gaacgtaacg acgagggtac
    27661 agattatcat cactattgaa gcgaacagga aaacatacgg tatgtattcc tcttcctgag
    27721 ttcctttgtc ccagccgcct attgcgcaga caatggttgc tgcctgcaaa gcgagaagac
    27781 tgccgactcc gcctacaagg ttgataaccg agttaccctc actgcgaagt gcgggcggcg
    27841 taaggtcggg cataagtgca acgcaaggtg ctcgccagaa tgacataaca aatacaaaga
    27901 gaataatgca aaacatcata gcggaaattc ttccgttttc atgcagagca actctcggaa
    27961 ttatcgcaaa aatgactgca gcgagaggtg cgccgataag tataaaaggc ttacgcttac
    28021 cgatacgggt atggcatctg tcagatattt ttccgaatgt cggctggaaa acaacgccga
    28081 atatgttatc aaatgtcatt ataataccaa tgaaaagagg tacaagggta aaacctgttg
    28141 ccgcagagcc tacgtcctcg ccctgagcct gcatgaattc aagaagaata gggaactttt
    28201 cgacaagctt tgcgctccag cctgcaatca gtgcggattc gccgaggatc ttattgagca
    28261 gggtggtgat atacgggtca taaacacccc atgctattga agaagccata aatgcaaaac
    28321 ctatcaaaag ggtcttgccg taattaagct tcattttttt tgctttttca gccataaaaa
    28381 caatcctttc ttatcatgga taacaaccga taaacacttc tcgattattg tccgtatcat
    28441 tatatcacga attgtcacaa aatcaaatgc ttttatggcg ttttttgcat aatttatgat
    28501 attatttttc catctaattt ttaaaattgt atgaatttgc ggaaaagaaa aagtaaacat
    28561 tacgttttga actgaaattg acaacaaatt cttgacaaaa acgaaaagaa agaatataat
    28621 ttttgtgtca aacgggaatg cattcaatca caccgcattg ccttaatatc cgggggcgta
    28681 gctcagctgg gagagcgtac ggttcgcatc cgtaaggtcg agagttcgat cctcttcgtc
    28741 tccaccaagc atccgccgta gttcttatag aagaattgcg gcggattatt gttttatttt
    28801 tattatatat aattagatag gagcaacagt ataatgaata tcagagcagc tcaaaggaag
    28861 gatatccctc agattaaaga attgttgagt caggtgcttc aaattcatgc agaaatccgt
    28921 cccgatattt ttgttccagg aaccacaaag tataccgacg cagagttaga agaaattctt
    28981 aacgatgaaa caaaaccgat ctatgtcgca gtggatgaaa acgataagtg tttaggatac
    29041 gctttctgcc agttgaaatc acagcctttt tcaaataata tgataccgtt taagtcttta
    29101 tttattgatg atctttgtgt cgataaaaat acaagagggc agcacatcgg agaaaggttg
    29161 tttgaatttg taaagtgcga agcaaggaaa aaaggatgct atgaggttac tc
//
LOCUS       DAWBSW010000004        28712 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_340_length_28712_cov_2.691245, whole genome shotgun sequence.
ACCESSION   DAWBSW010000004 DAWBSW010000000
VERSION     DAWBSW010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 28712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28712
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_340_length_28712_cov_2.691245"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4266..4275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10260..10271
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12329..12338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16012..16076
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16301..16310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8067 a         6149 c         6945 g         7444 t
ORIGIN      
        1 tagcttactt tgtaaaattt tgcgattctt ttcatggtta cctctttata tcagcttttc
       61 aacgttgctt tttcctgttg caagattaaa ctcaagtgtc tttttctttt ctccctcggt
      121 gtagcaaact tgaatttcgt tttcacttat aaagcctgcc gatgtgatga ggcactcatt
      181 ctttttgaag aactttttca aaagctcaat atcctcgtca ctgtaatcgc cggcattgtc
      241 cgagacgaaa agcacatcgc cgcagagatt gttgatcttg gcaagaagaa gcttttgctc
      301 ctttgtaaac gtgaggttct tttccctcag gaagaaaacg tcgggatcgt tgcagaatac
      361 tctgccgttg agatgacgtc ggaatacgga gttcgtaatt gcattttgag ccgagggaag
      421 ctcaagattg attcccattt tgttataata tttaggtaaa tatttgaggt cgacgtcgca
      481 gcttattctg caagcatcta caacgccgaa ggacggaccg agcggaacgc cgcaaccgag
      541 aatcaacttc tcctcaccga cgcattcgcg caggaagtcc attgcttcgc acattatttg
      601 acctcttgat tttccgtttc tcggctgcat tgcttctgaa tagagaaaat caagcttaac
      661 catgtcgaag ccccagtcgt tgagaatgac attgaagaaa ttgcgaatat actccctgac
      721 ttctccgttg taaatgtcaa gcgtatatgc gccgccccag cctacgcagc cgagcagagg
      781 cttgcccgtg gcattgtcct taatcagcca atcggggtga tccttagcga taacggaact
      841 tctctgcgcc gagaacggag caatccatat gcctgcaaga tagccctttg cgtgaattgc
      901 atcggcaatc ggcttcatgc cgttcgggaa ttttctgtaa tcgggaacaa gccagtcacc
      961 gaccttgctt tcatagccgt catcaacttg gaatattgaa acgctgttct ttgcacggtc
     1021 gaggccgtca agatcacgca gaatgatttc ttcgtttatt ttttgaaaat agttgtacca
     1081 ggaggtgtag cccgaaaggt gatcgacctt tggctttcta aggttcattg aaccgaaata
     1141 tttgtcaaaa acctcgttgt atccgccgtt gaagcaggta atgtcaagga gaatgatttc
     1201 atcgtcggtt tccagtccgt caacgtcctt ggcaattgta aatcggttgg tttcatagct
     1261 tacagagaaa accgtgtaac cgtatctctc atcacacgag ccgaaaagtt caaccctgtc
     1321 tccgactcgg aaataggtgt atgtatagga atgaaaaacg ccttttttac cgtactgcgc
     1381 aaagctgtag tcgcctgaaa tttccgaaag cgcctttgtt ttttcggtaa gcccggcaag
     1441 ctttgaaatg ccctcgagcg tatcgtccct gccgaactct ctcgacgttg tccatgactg
     1501 ataaccgttg gcaaagaaaa gctcgttttc gtcaaacttc ttaatagccg aaatactgaa
     1561 ttcgatcagc ttcattttaa cattcggagc aagcgtgcac tgtattccgt cgtcattgat
     1621 tgaaagctcc aaactgtagt tgtcacattt taatttactg ttttcgtaaa ttcgtccgtt
     1681 atattcgtat ttaataaaag gttcaaatct gtacatttca caacctccta agcattaagg
     1741 cgatactgca tacctcagtt gctcggtatc gtattaaaat aataacacat tttgcatggc
     1801 aatacaatat ttatttgttg cggcggaaat aaatcataaa caggttgaaa tttttggagt
     1861 tttactttat aatataatgc agtatgaaat gaaggaagta atattatgaa gttacgtata
     1921 gtaggcttgc cgaatgtcgg caaaagtaca ctttttaatg caataacaaa cgcgggtgca
     1981 cagtccgcaa actatccgtt ttgcacaatt gaaccgaatg tcggtgtggt tgccgttccc
     2041 gacagcagaa tagacgcact ggcaaaaatg tacaacccgg tgaagattac tcccgccgtc
     2101 atcgaattcg ttgacatcgc aggtcttgtt aagggagcgt caaagggaga gggactcggc
     2161 aacaagttcc tttcgcatat tcgtgagacg gacgctataa tacatgttgt tcgctgcttt
     2221 gagaacgatg atattattca tgtcgagggc agtgttgacc cggcaagaga cattgagacc
     2281 ataaactttg aacttatttt ctcggatatt gagatggttg accgccgcct tgacagaaca
     2341 atgaaagcga tgaagggcga taagagtctt gcaaaagaag ttgaattctt gaaaaacctc
     2401 aaggctcacc ttgaaagcgg taaatgtgca agaagctatg attgcgatga tgacgaaaaa
     2461 gcggttctca atgacatggc tctgcttaca tcaaagcctg ttatctatgc ttgcaacatg
     2521 agcgaggatg actttgcaaa caatatcgag gaaaacgaaa gataccgtgc ggtctgcaaa
     2581 atcgcagagg atgagggttc gcagactctt ccgatttgcg ccgaaatgga agccgaaatt
     2641 gcaagcctca gcaaggaaga gaaggaaatg ttccttgccg acctcggcat tgagcaggga
     2701 ggcctcgacc tcctcattca gcgcagctat gatttgctcg gactcatttc gtacctgacg
     2761 gcaggtcagc ccgaggttcg cgcatggaca attaagaagg gcacaaaggc tccgcaggcg
     2821 gcaggaaaga tacattccga ctttgaaaaa ggctttatca gagccgaggt gatcagcttt
     2881 gataacctta tggaatgcgg ctcgcttgct gttgcaagag aaaaaggcct tatccgctcc
     2941 gagggtaagg agtacgttat gaaggacgga gacatcgttc acttcctctt taacgtttaa
     3001 ttgaatattc acggcagccg cagaaatgcg gctgtttttc atatataaaa tcaacctccc
     3061 gaaatcaatc gggaggctgt gtttcatatt gaattatgat ttcatgctga tgcccttgct
     3121 ctcaagcttt gcaatgaggt cattgacaat tgccataacc tcgtccttaa caagagttct
     3181 gtcggggtgc gagaacacaa tccttatcgt gatagacttg ccgcttgcgt cctggtaagt
     3241 gtcaacaacc gatgtctttt tgataagctc ggaattgata agctcaattg cttctttaat
     3301 cggcttgaat gtatcggcaa cgaatgttac gtcaacctca attgacggat actttgatgc
     3361 ttcggtatat ttgatgcctg cattttcaat agcaacaaga tcctttacgt ccatttcaag
     3421 gaagacaacg cttgctttcc tgtcaatgtt tctgagaacc tcggggtgaa caacaccgat
     3481 aacgccaagc tccttgccgt cgcaaacgat tgcgttatag ttcttcggat gaatatagtt
     3541 aagctcggtg ctctttgcct tgaacgaaag gctcttgtgc ttaatatcgt caacaacaac
     3601 cgaaatcatg ttgataagct tgaaataaag ctcctcggtt gtttctgttt tgctgtagag
     3661 cgtgacagca agcttcttat gctcacggca gaaattattc tcgtcaacgc cgtcaacaat
     3721 gcgtcctatt tcaaagatac cgaacgaagg agcatatgaa gaattagact taacctggca
     3781 aagctgagtc gggataatcg accttctgat tgtttcgatg ttcggattgg tagcgttggc
     3841 aagcttaatg ttcggctctg tcgaaatgcc gagcttttta tactcgtcgg tgtattccca
     3901 gatatatgaa tgaacctcat gaagtccgaa ttcctttaca agaatgtcct taatcttgtc
     3961 ctcgatactc ttctcggcaa acggacgaac agggtatagg ggagcggttg ccgtgtgaat
     4021 atcaaagttg tcgtagccgt aaattcttgt gatttcctcg atgatatcgg ctttgagcgt
     4081 aacgtccttt gttgctctcc agctcggaac gtcaacggtg aagtcgtcac cgtcaagctt
     4141 aaccttgaat ccgagtgcgg taagggtctt aacgatcgtg tcgttgtcaa tctcaatacc
     4201 tgtgtatcgg tcaacgaatt tcttgtcaaa attgagttca acgttgtcga acttatgagc
     4261 gtactnnnnn nnnnnccttt actcccgagt cgtagtcgct gagaagctta acaaaacgag
     4321 caattgcggt aacggtcatc tcggggtcaa gactcttttc ataacgcatg gaagcatctg
     4381 ttctgtgagc aagacgagcc gaagattttc tgaccgaaac agcatcaaat gttgccgatt
     4441 caagtgtgag tgaatgtgta tcttcaacga tttccgaatc aagtccgccc atgatacctg
     4501 cgatggcaac gggtgtgttg tcgttgcaga tcataagagt gttttcatca atgtgtctct
     4561 cgacgccgtc aagggtctga aattcgaacg gagtgtcaaa tctcttgatt ctgatttttt
     4621 caacctttct tgagtcgaaa gcgtgcatcg gctgacccat ttcaagcatg agatagttag
     4681 tcaggtcagc gaggaagttg atggctctca ttccgcagta atagagacgg atcctcatgt
     4741 cagccgggct gacgtttctt gtgatattct caaactgaag gcatgaatat ctgtagcaga
     4801 ggggatcctc aatcttcata tccaccttcg ggagatcctt gtatcggctg aggtcaaccc
     4861 tatcaagcgg cttaagcggt ctgcctgcga gtgcggcaaa ttctcttgca attccgtaat
     4921 gaccccaaag gtcgggacgg tttgtaagcg acttgttatc aacctcaaag atgatatcct
     4981 caattgcaaa aacatcctta atatctgtgc cgttggcaag gctttcatca aggtccataa
     5041 tgccggaatt gtcgtcgctt atgccgattt ccttttcgga gcagcacatt ccgtacgaca
     5101 tatatcctcg gtgcaatttc tatctcgccg agctttgcac cgacctttgc aaatgcggtt
     5161 ttcataccta ctcttacgtt cggcgcgccg caaacaacat ctgtaagctg tccgtcgcct
     5221 gcatcaacct tgagaagatg aagcttcttt gattcggggt gctcttcgac ggatttaatc
     5281 tcggcaacaa cgattccgct gatgtcgctg cccttataga atatttcgtt ttcaacctcg
     5341 gcggtggaca gggagaactg atgaataagc tcattgatgt caagacccga aaggtcaacg
     5401 aaatctttaa tccagttaat tgaaataaac attgctttgc ctccttattc gtcgtccgtg
     5461 aattgctcaa gattaacaag agcgccgctg ttgaggtcac ggatatcctt gatgccgtac
     5521 ttcatcattg caagtctggt aagaccgaga ccgaatgcaa agcctgtgta ctcgtcggga
     5581 tcaatgccgc cctctctgag tacgttcggg tgaatcattc cgcaggggca aagctcaagc
     5641 cagccgctgt gcttgcagga cgggcagccc tcaccgccgc agatgaggca gttgatgtca
     5701 agctcaaatc cgggttcaac gaacgggaag aagcccggac gcagacgaac tttaatatct
     5761 ctttggaaaa cctcggaaag catggttttc ataaagaaaa tgaggttgga tattgaaata
     5821 tccttgccaa ccataacgcc ttccatctgg aaaaatgtgt tttcgtggca ggcgtcggtg
     5881 ctctcgttac ggaagcatct gcccgggaaa acagccttga tcggtttgcc ctcgttgatg
     5941 agctgatcct tgtatttttt atagatagcg ttctgagccg cagaggtctg cgacttgaga
     6001 agctgaccgt ttgtcagata atatgtgtcc tgcatatcac gggcggggtg gaatttcgga
     6061 atattgagcg actcaaagca ctcgtagtcc gttacgacct cgctgtaatc ctcgacggtg
     6121 aagcccagtg atttgaacac gttttcgcac tcgcgctgaa ccagcgtgat cgggtgatat
     6181 gaacctcttg tgagattcgt cggaacagaa acgtcaaatt cgggggtcat ttcaagctct
     6241 cttttaactg caagcttatg aagctcctca ccctttttgc ttatcgcttc ggcaacgctc
     6301 tgcttaaagg tgttgaccgc ctgacccatt ttctttttct cgtcgggtga aagcttaccc
     6361 atgcccttga gaagtgttgt tacaaaaccg ctttttccga ggaaattgac tcgaagcgtt
     6421 tccagctcgg aagcgtcctt gacctgcaaa atagagtctt caaacttttt tctgagcaat
     6481 tcaatttgtt ctgccattct ataacctcct gataaaaata aaacccatcc tataacaaga
     6541 tcgaaaccct gttatgggac gggataaacc gtggtaccac ccatattcgg agccgtggct
     6601 ccgcactcct tagccgtcat gatgcggacg gaaacgccct gcttaatcaa agctttcgca
     6661 ggtcactccg atgctgaaat tcgtttctgt ccgtaaggcg gtgctttcat ccgatggcaa
     6721 ccgctctctt tgcttacctt aacaaaaact acttacacat cttcaacgtg tatcgtcacg
     6781 gactatctta acatcttaat tcatttttgt caagtgataa tatgcggcaa attatgcttt
     6841 atttatttta cgaacggggc ggacatattt ccaaaagcgc ttgcagtcgt gatagaaata
     6901 aaatactctg cccgcttctt tgtcaagctc ataacccgat gcggcgtagt cgaaatcctt
     6961 catcgcctgc tcgattttct gctcaacggc agcgttctcg gtcgcaaaat caaacggacc
     7021 gtgttcaaaa acaagatatt caccctcggg tacgtccatg atcatcattt gcggcggaat
     7081 ttcaccgtca tagtcggcag gcagtcttac accgtaagcc tcggcgagcg gaattcccca
     7141 actgcaaatt ctgccctctg gctcgttgat aaatgccatg acctgaccgt tgaagctgtc
     7201 gttatcttct ccgccgacgt aatcaagctt gccctttatg ctgtcaagga gaccgcagat
     7261 agtttcgcag tcctgaccgg ggattttgct ttgcttctgc caaaaatccc aatagccgat
     7321 gctttcatag tttcttatgt gcaaaaattt gtgtgcggga atagttataa aatatgtttt
     7381 tatttcatga tttgtctctt tcataccaaa tcctccgata tccataagat agcagtcaaa
     7441 gggtctcaga attgtacgaa gaacaacggg tacgggattt tttcggtact cactggggtt
     7501 tattccgcag gcgtcacgga aggctcttgt gaatgcttca tgtgacgaaa agccgtaatc
     7561 gagagcgata tcgagaattc cgcggtcggt atctctcagc tccctgagtg caaaggcaag
     7621 ccgcctgtat cgcatataat cacgaagcga cattccggaa atttcgctga actttctcga
     7681 aacataaaat tccgagtagc cgagattttt agccagccgg ccgagagcca aagactcccc
     7741 atcgtgattt ttaatgcagg cgtctatttc gtcaatgata ttctgaatgt tcctgtacca
     7801 atcgtacatc gcacttattc cccctaagca accgttgatt aaatctgcgg ttacctgata
     7861 tgtctattat atcggaattt acaagtatat gcttgattta agttgctgta ataaatatgt
     7921 tggattttct gtgtgatttt aaaaactatc agttaaaaaa cgttaagtgt tatatctcct
     7981 tctgttcaag gatcattcgt tcaagctctt ttgaagccat gctgagcgac tggtcctttc
     8041 tttttatgag tacaacgttt cgctcgggga ttttttcctt taggtcgatt atgcaagcgc
     8101 tgcttttctc ctgcaaaaat tcctcgggaa caaagccgat tccgagatca gcttcgacca
     8161 tgggcaggat ttgatctgcg gtgaaggctt caatgtccgg ctgaaacggc agtccgtgag
     8221 ctgtgaaaag cttggaataa aattcaaagg attttgtatt cgtaccgagt gagataagag
     8281 gataatcaat aagctcggaa agcgagactt ttctgccgat aagagatgaa aatgactttg
     8341 agcatacggc aacctcattg acgggcttga cggttgtttc cgaaagagat gccgaacgaa
     8401 cagtcggagt tgtgacaacg gcaatatcgg cagttccctc tctcagcgcc gcaacggctt
     8461 gcggagttga atggttactg attttaatat gtataccggg gtaaagcata cggtattttt
     8521 tcagaacggg cagaagcagg cagtgcagtg cgacctcact ggcggctaca tagaccgtgc
     8581 ccgtttgcat atttctactt tcggtcagtt cggcttcacc ggcttcaatg tgctcaaaag
     8641 caatacggat atggctgtaa agccgctccc cctcgggagt cagcttcatt ccgctgtgac
     8701 ttctgagaaa cagcggacac ccaagctcac tctcaaggtt ctttattgaa cgtgtgagat
     8761 tcggctgatt atttaataaa agctttgcag cctgagtaat atttccgtac tttgcaacat
     8821 agtaaaaaat tcggtagtag tcatagctga tatacataaa gccacctcgt atatacaaaa
     8881 atgatatgat aaacatataa atcatagatt ttacatatag ctacaagcgc attataatat
     8941 agccgtactt gaaagtcaag gacaattatt aatcggattt taagggtcgt gttactgatg
     9001 aattacaaga atgcagatat gtcggtgttg gctgatgaaa tgaaagactg tcagaagagg
     9061 cttgacgaat atgttaagag cggtatttca cttgaccttt cacgcggtaa gccgtccgcg
     9121 gaacagcttg agctgtcgct gaaaatgctt gacgtattgg atcatcgcag cattcttgac
     9181 agcgaaaacg gaacagactg ccgtaattac ggcggacttg acggtattcc tgaggctaaa
     9241 cgtctgctgg ctcatattat gggtacacat tcggcgaata caattgtcgg cggaaacagc
     9301 agccttacaa tgatgtttca gattattagc cacggcatga cggacggaat atgcggcaat
     9361 actccgtggc aggaggtcaa ggaccgcaag tttctttgcc ctgtccccgg atatgaccgc
     9421 cattttgcaa taaccgagca ttttggcttt gagcttgttc ctgtaaagat gaacgatgac
     9481 ggacccgaca tggacgaggt cgagaaactt attaaggacg ataaggttaa gggcatatgg
     9541 tgcgttccga agtatcagaa tccgatgggt attgttttca gcgatgaggt ggttcgccgt
     9601 tttgcacatc tgaaacccgc ggcagaggat ttccgtattt tctgggacaa cgcatattgc
     9661 gttcacaatt ttgacggcga atgcgctgag atccccgata taatcgaaga atgtcagaaa
     9721 gcaggaaacg cagacatggt ctatgagttc tgctcaacga gtaagataac ttttccgggt
     9781 gcaggtattt cggctgttgc ttcaagtcct gcaaacctta ttgatctcaa ggcattttta
     9841 aaatatgcaa cgatcggtcc cgacaagctt aatcagctcc agcacgcaag gttctttaag
     9901 agcagtgccg gacttcgttt acatatgaaa aagcacgctt caattctcaa accgaaattt
     9961 gacgctgtgt atgaaacgct tgatagggag ttaggcgaca gcggaattgc cgactggaca
    10021 aagccgacgg gcggatattt tctttctttc aacaccatgc ccggctgtgc gtcaaaggta
    10081 gttaaaatgt gcgccgactg cggagtgaaa tttacaccgg caggttcgac ctatcctcac
    10141 ggtattgacc ccgaagacag aaatataagg ctggcaccgt catttgcaac ggttgaggaa
    10201 ataaccaagg ctatcacaat tttgagcctt tgcacaaaga ttgttacgat tgaaaagatn
    10261 nnnnnnnnnn ngtcaaatgt ccgaccattt ccgcacggcg ctgtttatca ttctttcggg
    10321 cggatttcag gacgcatata catatatatc gcgcggcggc gtgtttgcca atgcacagac
    10381 aggtaacatt gtgctgatga gctcggcgat gtttgacggc gatgcaagca aaattttcaa
    10441 ataccttatt cctgttgttt cgtttatact tggcatagcc accgccgagg ttgtgcacat
    10501 gcacttcaag cattacgaaa aaatacattg gcggcagatg atactcttct cggagattat
    10561 tctgctgttt gtcgtcggat ttcttccgca tcgggtcgat tatcttgcaa atgcacttgt
    10621 ttcatatgtg tgcgccctgc aggttcaaac attccataag ataagaggtc acgcttacgc
    10681 aagcacaatg tgtatcggaa acatgagaag cggcacggag gcactgtgtg tatattatca
    10741 tacgcatgat aaagaggtgc tcaaaaaggc actgacgtat ttctcggtaa ttctggtctt
    10801 tgccgtcggt gcgggaatcg gaagctttat aacaaagatt gcaggagaca aatcaatctg
    10861 gatttgctgc gtcctgttgt caataagctt cggtatgatg tttgtaccgg aaaaagaata
    10921 aaaggagttt ttgaaaaatg ggcaagatta aaaaagttgt actggcatat tccggaggtc
    10981 tcgatacctc aattatcatt ccgtggctta aagaaaatta cggtaactgc gaggttattg
    11041 ccgtttgtgc aaatgtcggt cagacgggtg agcttgacgg acttgagaaa agggcaaagg
    11101 cgagcggagc ttcaaagctt tacattgaag atttgacaga cgagttcgtt gatgattatg
    11161 taattccgac catgcaggcg ggtgcagatt atgagggtta tctcctcggt acaagctttg
    11221 cgagacccgt aatcgcaaag agacttgttg aaattgctaa ggccgaagac gcagacgcta
    11281 tttgccacgg cagcacaggc aagggcaacg atcaggtccg ttttgagctt gcggttatgc
    11341 actttgctcc gaaaatgaag attattaccc cgtggcgtga atgggatatt cagagccgtg
    11401 atgaggaaat cgactatgcc gaggcgcaca atattcctct taaaatcagc cgtgagacaa
    11461 actattcaaa ggataaaaat ctttggcatt tgagccatga gggtctcgat cttgaggatc
    11521 cgtcaaacga gccgcagtat gagaaacacg ggttccttga gcttggcgtt ttcccgactc
    11581 aagcacccaa caagtcggaa tatgtcgaaa ttgattttaa aaagggcgtt ccgattaagc
    11641 tgaacggcaa gacggaaaaa gcatccgaaa taataaaaga attgaaccag attggcggta
    11701 agaacggtat cggtctttat gacgttgttg aaaaccgtct tgttggaatg aaatcgagag
    11761 gcgtttatga gaccccgggc ggaacaatcc tttaccgtgc ccatgaagtc cttgaaacgc
    11821 ttgttcttga cagagatacg cttcataaga agaaggagct tgctgttacc ttcggtgagc
    11881 ttgtctatga cggaaaatgg ttcactccgc ttcgtcatgc gctttctgct tttgttgagg
    11941 atactcagca gaatgttacc ggtaaggttc gtttgagact ctataagggt aatattatta
    12001 acgccggcgt ttggtcgccg tactcactct atcgtgagga tattgcaaca ttcgatgccg
    12061 gcggagaata ccgtcagtcg gatgcgacag gatttatcag cctttacggt ctgccgacaa
    12121 gagttcaggc aaccgttgat gaagaaaata aaaataaata atcaaaggag aagatcaata
    12181 tgctttcggc agttgtagga attaactggg gtgacgaggg taagggtcga attgtcgatt
    12241 atctcgcacc gaaatacgat gttgtttcaa gatatcaggg cggcaacaat gcaggccata
    12301 cagttgttaa tgacaagggc aagtttatnn nnnnnnnnca aacgatacgt acgctgctat
    12361 aaatttgatt atatgcgttt ttttaccttg acaattattc gacaaaatga tatattattc
    12421 gatggcgaac gccacaaata catatttttg cggaggctta tatgtcagta acggctttag
    12481 tcggttcaca gtggggcgac gaaggaaaag gtaaaattat agacatactt gcgtcggaag
    12541 cggacgtggt agtacgagct cagggcggca acaatgcagg ccatacagtt gttaatgaca
    12601 agggcaagtt tattcttaac cttttgccct cgggaattct tcaggacgga acggttaatg
    12661 tcctcggaac aggaatggta attgatattg aacaccttgt caatgaaatc ggcaagctgc
    12721 gtgagggcgg aatcaagatt tctcccaaaa atctcaaaat cagtgaccgt gcaacgattt
    12781 gtatgccgta tcaccgcttg caggattgcc ttgaggagga cagactttca gataaaaagt
    12841 tcggttcaac aagacgaggt ataggacctg tttattcgga taaatacatg aaaaagacca
    12901 ttcgtatggg tgacctcttt gaccttgaaa gaatcaagga gcatctttat gacattgttg
    12961 agtggaaaaa tcttataata gtcggcggat atcaccatga accgattgat gccgaggaga
    13021 tgtacaactg gctcaaaacc tacggcgaac cgctgcttcc atatatttgt gacacgtcgg
    13081 aatacctcac aaaggctgca aacaaaaata agaacattct ttttgaggct cagctcggtg
    13141 cgatgagaga tattgacttc ggtatttatc cttacacctc atcgtcaaac agtatcgctg
    13201 ctttttcaac aataggcgca ggtattccgc aagtcaatct tgatgagtgc ataggcatta
    13261 tgaaagcgta ttcaacctgt gtcggcgaag gtccgtttac ttgtgagctt ttcggcgatg
    13321 aggcggagaa gcttcgaaag gccggcaatg aatacggagc ggctaccggc agaccgagaa
    13381 gagtcggcgg atttgatatc gttgcttcaa aatacggcgt aagagttcag aacgcaactt
    13441 atattgcgct gactaagctt gacgtgcttt cctatatgga caagataccc gtctgcgttg
    13501 catatgaata tgacggaaaa actatagact tcttcccgtc cggcaatgtg ctaaacaaag
    13561 cagtgcctgt ctatgagtat cttgacggtt ggaaagagga tatctcaaag tgtcgttcat
    13621 attcggagct tccggagaac gcacgcaaat atattgaata cattgaaaag aatgttggtt
    13681 gtaaaattaa gtatatttcc gtcggtgcag aaagagacgc tttgattgaa aaataaacct
    13741 tcaatattac ctaaagcaaa ggacagagac ttcggtctct gtcctttgcc gtgaggacgc
    13801 ataatgagtt tattatatat tgacacttat gttataatct ttccaaaggt ggtaatgcga
    13861 tgaacaagat tgaacttcga gaaaagtgca gagagataat taacggcaca cctcccgaaa
    13921 aatttgttga atggggcgag aaaatttccg ttaatgtgct cgcttcaaac gagtggaaaa
    13981 atgccgaaac ggtgttcgtc tttgcttcaa tggggaacga acccgataca aaactgttga
    14041 ttttgaatgc aatgaaggag ggcaagcggc tttgcgttcc gagaataacg ggtgacggca
    14101 caatggaagc cgttgaaata aaaaattttt ccgcgcttaa aaccggaaag ttcggaattc
    14161 ccgaaccggt cgatggctgt aatgctgttg aaaaaaacga gatcaatttg atcttgcttc
    14221 catgctttgc tgccgatgag agcggaaaca gactcggaaa gggcggcgga ttttacgaca
    14281 ggttttgcga aggaaccgat tgcggaaaaa ttataatttg tcccgaattg tttttgatga
    14341 aacaaggagt aatttcgaca gaagagcatg atgttgccgc agataatgtt gcaacagaga
    14401 aaaaatttat tagagtgagg cgataaatat ggatttcaaa aagagtttta acagggtcgt
    14461 aacgaatgtt gttcagttct ttgcaaacgg actttcacag agcgcgaccg acagggacgc
    14521 tgaagaaaaa gaagtaactc cgggtatgcc ggagcttctg agacgcgcgg cggcagaggg
    14581 agctgttctg attgaaaaca atggagttct tccgctgaaa gagggcacaa aggttgcact
    14641 tttcggtata acgggatatg aaagtcatta tgtcggatac ggctcgggcg gagatgtaaa
    14701 caagccgtat gctgtcagct ttgccgacgg aatcgaaaac tgcgacaggc ttgaaattga
    14761 caaagtactt gaaagcacat acaaaaactg ggacaagcaa aacccgataa atcacggctt
    14821 ttgggggcac tggccgtttt atttcccgga gatgccgctt gataaacaaa cggtcaggga
    14881 atctaaatcc cgaaatgatg ttgctgtggc ggttatcggc cgttcggcag gtgaggacag
    14941 ggattgtaac cttgaaaaag gcagctatta ccttgccgat gacgagattg caatgctcga
    15001 tatgatagcc gctgagtttg ataatattgt tattctgctc aatatcggcg gaattacgga
    15061 catgacatgg gtcgaaaaat acagggataa aatcggcgcg attctgattg tgtggcaggg
    15121 cggcatggag tgcggcaatg ccgcggcaga cctgctatgc ggcaacgttt ctccgagcgg
    15181 gcgtctcacg gatacaattg caaagcgcta tgaggattat ccgtcctcgt caagcttcgg
    15241 cggcaaggac tttaacgagt ataaagagga tatctatgtc ggctacagat atttcgagac
    15301 ctttgccaag gacaaggttc tttatccgtt cggctacgga ttgagctata ccgattttga
    15361 aatatcttcg aatgtgtcag aagaaacgga caggggcttc agattttctg tcagcgtaaa
    15421 aaacatcggt cactcggtcg gcaaagaggc cgttcagctt tacctgcgta agccgtgcgg
    15481 caggctcggc aatcccgaga gaattcttgt cgcttttaaa aagacgggcg agcttgcgcc
    15541 gaacgaagaa gcaagacttg atttgttcgt cgaaaaatct cagctttcgt catacgacga
    15601 ggacattagc gcctacgtta tcgagagagg aaaatatgaa tttttccttg gaaagaatgt
    15661 ccgagacaat gatttgattt acagctttga acagacagat gaagaaataa tcagtcagct
    15721 cgctcagtcg tccgctccga ttgagaagtt tgaagttatg aaagcctgcg aggaaagcgg
    15781 caaacgggtt ataaagcaaa gaactgtaag aacaagagaa tatgacctcg gcaaacggat
    15841 acttgaaaac ctgccgaaag caattccgca gacgggcgat aagggcttga agctcagcga
    15901 cgttaagaac ggtgaatgca ccatggagca gttcgtcgct cagttgagca acaaggagct
    15961 tgaagcgata tcgagaggcg attatgtgat gaacagtccg ctcggcgcaa annnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttct
    16081 tcattcattg agagcaaagg gcgttgagcc gataacgacg acggacggcc cttccggcat
    16141 ccgactttcc gcaagctgtt cgcttatgcc tgtcggaaca ctttttgcct gctcgttcaa
    16201 ccccgagctt gtagaggagg tttattcggc aattgcggct gaaatgaaag caaaaggctc
    16261 cgatgttttg cttgcaccgg gaatgaatat tcacaggaat nnnnnnnnnn gtgaggaccc
    16321 gtatttggcg ggtaagaccg cagcggcagc agtcagggga atacagtcac gcggagcatc
    16381 ggcttgtccg aagcattttg cctgcaacag tcaggaaaaa cgccgcaata tgaatgattc
    16441 acgtctttcc gagcgagcac ttagagagat ttatctcaag ggctttgaaa tctgcgttaa
    16501 ggagtccgag ccgaaaaata tcatgacctc gtataaccga ataaactcgg tttacggtca
    16561 ttataattac gaactttgca caacgattct tcgagatgaa tggggctaca agggcaacgt
    16621 tatcaccgat tggtggatga aaagcgagag aagtcccgag ttcccgtcaa tcagggatca
    16681 ggcataccgt gtacgggcgc aggtagatgt gcttatgccc ggcggaggaa ggtcaaaacg
    16741 ccgcagaccc gacggaacac tgcttgaatc gctcggcaag cagggcggaa taactctcgg
    16801 cgagcttcaa cggacggcaa taaatgtctt gaaagcggtt atcgacataa aaatataaat
    16861 ttcacttgtg caatcataca aatttgtaag gcaaaatccc gtttaggttg attttgcctt
    16921 tattatttga taaaataaaa atgattacga actaaatatt cagaggaaga gttaactatg
    16981 aaaaagataa tttcattatt gatggcaacg gttattgtta taggctgtct gcccataaca
    17041 acatttgcgg catataataa cggcggaaaa gcatattccg aaaacaccga tgaggtgtat
    17101 ttcagctccg gatatgctca ggtcggtgtg ccgataagcg taaaggtcaa ggaatccgac
    17161 acggatattc tctataaatg gtatataaac ggcgacgaga taagcaacag cggcgacagc
    17221 tacaccccgg ttgaggccga tcttgagtcc atgctgaccg cagaggctta cggcgctgac
    17281 ggcgaaaaaa tcggagcggc aaatatgctc ataagcaagc tgcccgttat ttatatcgaa
    17341 acgaaaaaca gggagcagat aactgttaag ggtaaggttt tgaaggctca tatgaagatt
    17401 cagggcaatt ctgagtttaa cgatgcttcg gttctttatg acggcgaaac ggatataaag
    17461 ggcaggggta attcaacatg gcttgcgaat aaaaagcctt acaaattgaa gctcgcttca
    17521 aaatcaaatc tgctcggaat gggaaaaaac aagcattggg ttttgctgtc caatccttat
    17581 gacaagacgc ttttaagaaa taagctgacg tatgaccttg cggaggaaat gggtcttgat
    17641 actatgagct cgcagtgggt tgacgtggtt ttcaacggca aggttgtcgg caattatctt
    17701 ctttgtgagc atgttcgcat aggcagtacc cgcgttgaca taactaactg ggacgatatc
    17761 gccgaagacg tggcaaaggc catttacaag gcaaataaaa aatccatgag caaggatgag
    17821 cgtgacgagc tttctgcgct tatggaggat aatatgaact gggtgaccga cggctcggtt
    17881 acatataagg gaaaagctta taaaatttcg gattactatg agttgccgag tgcaaacgga
    17941 ggctatctcc ttgagtgctt cggttcggaa aaaccgcgat ttgtctcaaa gggcggttac
    18001 ggagtttgca taagcaagcc cgagggtatc ggaaaaggta tgctcagtga gattcaagcc
    18061 tattattctg cctttgagga agcgacgcag gcagaggact tttgcgccga atataacgga
    18121 aaaaaggtga gatattccga gcttgccgat gcagagtctt ttgcaaaggg cattttattt
    18181 aacgaaattt ttcataatca ggatttcctg cgggacagca catatatgta caaggatgtt
    18241 gacggaaagc ttattttcgg tcctgtttgg gatatggata taagtgccga taattcatca
    18301 tatatatatc cacacaactc ttgggtttgc tttaccagag ggaatattct gaagatgctc
    18361 agagatcctg cgtttttaaa gttagtttat gacgcataca gggaatatcg ctatacaata
    18421 attgaaaaat ttgttatgtc gagcggttat attgacacgg cacacgaaaa aatctatgaa
    18481 tcgggaattg ccaacgacat gctttggaat acatatgcga gctttgaaga tgacacggaa
    18541 aatttaaagc tatggctcag ccgcagactc gattggatag acagccaaac gacgagcttt
    18601 gagacttttt actcctctgt taacggcaca aagctcaata attcgggcag cacaatgctt
    18661 gagctgaacg gctcacagct tgaaataacg atgaacgata ctggcattac agcgtgtaag
    18721 gtgcttgtga acggtacgga ggttaaaaca ctttcatata atgcacatac ctcggttaat
    18781 ctcggtacgc ttgcggaaaa cagcgttgtc agcgttatga gctatgacgt aagcggtaat
    18841 ttcctcggta tgtcaaccgt gacgagctat aacgaacctg tcagtctgaa aatgacaaag
    18901 gctccgacca agaacgtgta taattcggga gataaaatcg agctcaacgg acttgaactc
    18961 aaggcagttt atgccgacgg gtcggagaag accgttcagc ccgagtcggc gctttcatat
    19021 gttgaagatt gcatgggagc acaggagtcc gtgtacaatg agataaccga caaaatcggc
    19081 aatacatata tttcactcag atatcgcgga ctgaaggtcg attataaaat aaataaaaat
    19141 ccgaacgagg acgccgaaaa ggttgcgaag cttattgaaa tgcttcccgt gaataatttt
    19201 gaggataatc tcgaaaatgt tttcaatgcg aagcaggagt acgatgcgct cagcgacacg
    19261 gctaagaaaa aggttacgaa ttcttccaag cttgaagcgg caatgaagaa aatcaatgag
    19321 ctttcaaaga acagcgatac cccgatcctc ggctgctata tcgaccgtta tgcccgtgtc
    19381 gggcagaaga ataaggttgt ggttattgca aagggcaagc cgaacaaaat aagatttttt
    19441 atcggcggta gcacgactac aattcccgtt gcaaaccgtg attattgcat ctccgagaag
    19501 cagattgccg gctacaatgt tatgacgata gtttattatc tcggcggcag tgatgttgat
    19561 gtcggagcat attataatca tattcaaaag ggcgaattct atcatctcaa tgtaaaaaaa
    19621 gcaattgaaa accgcagcgg aatgataaaa aatatccgct ataacaagtc gacggcttcg
    19681 gcaggcgaaa ttgccgagct gaatgttact gttaataacc gtgttgaaat gctgacagca
    19741 acctgcgacg gcctcgaatt tgcggctgtt ccggaaaacg gcaaagcaac gcttgagata
    19801 actcctgccg tatcgggatt gaagaatatt gaaatcagct atattgcgga cggtgagctt
    19861 cacgaatata aaaccgtttc gctctatgtc agggagaaga aagccgatga agccttcacg
    19921 gcactcagat tcccgtcggc tacagcagag gacacggctg atgtatttgc tgccgcttcg
    19981 cagagcgtta aatccgttaa gctggtttca aagaacgaga cgcttataat gaaatcggct
    20041 cagaagaacg gattcaaaat ttggcaatgt aatgtcaaga tggattcaag caaatcatat
    20101 agggtttatg tcgattcaaa ggatatcggc agaacggtaa atattcaaaa gcttgataag
    20161 cttgtgattg aaaacggaag gctcgttgag tgccgtgtta agaacggaaa tattgatatt
    20221 ccgtacggtg taacgtctgt tgccgacgga gcatttaaag attttacagg aacggtttat
    20281 tgctataaaa attcagcggt gcaaggctat gccgagaaaa acggactcaa gtttgtcaat
    20341 tacggatata aaataaacat tcctgatgag attaaaatga aagcaggcga gagcgtttca
    20401 ttcgctccga ttgcagaccc ggttattgct cccgatttcg gcatgacaat aactgccgac
    20461 aatgcgccgc tgatttcgca aatcggaaac ggcttcaagg caaataaggc aggctatgca
    20521 agggtgacgg taaaatgcaa tgacggcatg tcaaaggtaa tcaagctgta tatcggaggc
    20581 ggctgcacaa agggagacgt caatgccgac ggtgtaatca attccgccga tgcgcttctc
    20641 attctcagaa atacggttgg attgatcgaa ttcaacgagg acgagaaaaa tgccgctaac
    20701 gtcaacacgg acggaataat aaattccctt gacgcactta tcgttcttca gataaccacg
    20761 ggaataagtt caatttggga ttttgtataa aaataaacga ctataaagag atgagccgca
    20821 gcaccaaaag tgcacggctc attttttcca ttatatatat tatttagttt ttttatttag
    20881 tttttagctt tacaaggtca cggtagctga taagcttgat attatgctcg tcaaaggcct
    20941 tgtagatgtc ggggtctttc ataatgagat actcccagta tcttcgtttc catcttccgg
    21001 tgatggcttt catctcgtcg gtagcaaggg cggggtgtat atatgtctca cagatcccgt
    21061 ccggaatgtc cgtaagatat gtgatgaaat tgtgtttaaa atcctcatag gattcgccgc
    21121 tttcaatgct ctcggggaaa atgagataat ccggcatcgg aacatgattt attttgccga
    21181 atatacccga gaaacggcat gccgctttga aaagccacat cggtgtgccg ggaggacact
    21241 gggattcaag aggctttgaa aacatacgga acggatagcc ctttttgccg cagattgcga
    21301 gcgttttcgg aagcattttc agcttgccgt tgagtccgta aagagcgccc atatgcgagt
    21361 caacgtgcga aggcttcatg cccttctttt cacagtattc gatttgagca agagtttcgg
    21421 cacatgcgtc gtcatatgaa cagtgagcct caaagtcctc actttcgggc cacattctgc
    21481 cctcgggggt tctgatgctc ttgccgtcgg tcaaaggacc ccacgggaat ctgtccatcc
    21541 actcgttagt gtgggtgaga tggactccga tagcgtactg agggtgtgtt tttgcaaatt
    21601 cgattgcttc gtctgcgccg gggcacggag ccattattgt tgaggaaagc agacagccgc
    21661 tttcaaaaag atccatgcaa gcctcatttg ccgaatggca aaggccaaag tcgtcggcgt
    21721 ttataataag atatttactg ataattatca ctcctgtttt ttctcgatcg gagaatgact
    21781 gtataaccat acatatttta ctatatcata caaaaaaaag caagcacttt aaatatacaa
    21841 accgcttgaa aaatataaac gtatattata taattaaatc aaagatcagt ctgaaaaggg
    21901 tgacagccgt gagaaaatat gacataaccg cactcggcga aatacttata gactttgtac
    21961 ccgacggggt tgattctgtc ggcgatatgc gctttatccg taaagcgggc ggtgcgccgc
    22021 tgaatgtgct tgcaactgcg gcaagggcag ggcttaaaac ggctttcatc ggcaaggtcg
    22081 gaaatgatct tccgggtgaa tttctgattg aaaccgtcaa gggctgcggc atcgactgta
    22141 attacctcaa aaaggacacc gagcacaaca ctacccttgc ctttgtcgag cttgataata
    22201 aaggagacag aaagttcagc ttttacagaa cttacggtgc agacagattt atttatgaat
    22261 ccgacgttga caaaaagctg atagccgact cgaaagtatt tcatttcggt tcactttcat
    22321 tgacggacga accgtcatat ttttcaacgg agatagccgt caaaaccgca aaagattgcg
    22381 gctgtaccgt gacctatgat ccaaattacc gtccgcccct gtgggacagt cccgaaaagg
    22441 cggcggagcg catggcggtt cttcttccgt atgtcgatat cataaagctt tcggttgagg
    22501 agcttgaaat gataagcgag ggcaggggaa cggaattcct ctttgaaaaa ggagtaaggc
    22561 ttgcactggt aacggacggt gacaagggag caacgctctg cttcaaggga gaaaaagctc
    22621 acattccggc aattaaagcc aaaaccgtgg acacgacggg cgccggagat atcttcttag
    22681 gcactttcgt cagcgaattt atcaaaaaag gtgaaaatct tgacgagctt tcgcttgacg
    22741 atgcaataaa ttttactcaa aaagccgtat ttatttcggg caaaagcact gaaaaatacg
    22801 gcgcaatagc ctcaatttct gatgtgatta tataaacaat tcaggctgtt aattccttcc
    22861 gtcaagggta tatcgatata tgcaataccg atatgagaaa gaggcaattc ccaaaacgag
    22921 aaaactttga taaaataata acaatcaaag gaacgacata cgttcatggg aggtgtcatc
    22981 ttgaaagaga cggagatatc acggccggca ctcggcagaa ttccgatgta tctcagattt
    23041 ctcaaaggct tgcccgagag tatgcagaat atatcctcaa cggtaattgc caaggagctg
    23101 ggactcgggg aggttcaagt aagaaaggat ctcggcgcat tgtgcggttc gggtaaaccg
    23161 aaggtcggat atcagcggga agaacttatc gggagtcttg aaaaattcat gacgggcgat
    23221 aaggtcgagg cagtaatcgt gggcgcaggc agactcggaa aagcgttgct tgactattcg
    23281 ggcttcgagg atttcggaat ttcggttctt gccgcttttg acaaaaaggc gcaggaggac
    23341 acgatatccg agggaggcaa gccgataatg cagatgtcgg agttttcaga cttctgcaag
    23401 gtgcataata tcaagatcgg tattattgca gttccgtcgg aatccgctca gtcggtgagc
    23461 aacctttttt acgagaacgg tgttcgactg atgtggtgct ttgcaccgtg tcagctctac
    23521 aagccggcag acgcagttat tcagtatgaa aatcttgcgc tgtcgcttgc acatttgaaa
    23581 atgcagataa aagaatcaca gacaaacctg accgacgcaa aacggcggtc ataagaaacc
    23641 gggaggttaa gatatggaaa caacagaaag aaaaatcgtg gacagtgttg aaacactgga
    23701 ggaaacactc gcaagagtta aggaagcgca gaaaaaattc gctacctaca ctcaggagca
    23761 ggtcgacaag attttccttg ccgctgcaat agccgccaac caggcaagaa ttcctcttgc
    23821 aaagatggct gttgaagaga caggcatggg cgtcgttgag gacaaggtta tcaagaacaa
    23881 ctatgcagca gagtatatct acaatgcata caagaacaca aagacctgcg gcgttatcga
    23941 agaggataag gcatacggca tcaagaagat cgcggaaccg atcggtgtta tcgccgctgt
    24001 tatcccgaca acaaacccga cgtcaacggc tattttcaag tgccttatcg cactcaagac
    24061 ccgaaacgct attatcatca gcccccaccc gagagcaaag ctttcgacta tcgctgccgc
    24121 aaagatcgtt cttgaagccg ctgttgaagc gggcgcaccc gaggagatca tcagttgggt
    24181 tgacattccg agccttgata tgacaaacct tcttatgaag gaagctgata tcatccttgc
    24241 aacaggcggc cccggaatgg ttaaggctgc ttattcatcg ggcaagccgg cactcggtgt
    24301 tggcgcaggt aacactccgg caatcatcga cgagacagcg gacatcgtcc ttgcagtcaa
    24361 ctcgatcatt cattcaaaga catttgataa cggtatgatt tgcgcttccg agcagtcggt
    24421 catcgttcat cagaatatct acgacgcagt taaggctgag tttgctgaca gaggctgcta
    24481 cttccttaat cccgaggaga cagagaaggt cagaaagacc attatcatca acggctcgct
    24541 taacgctaaa atcgtaggtc agcccgctgc gaagattgca gctcttgcag gcgtaacggt
    24601 tccgaccgga acaaaaatca ttatcggtga ggttgaaagc gtggatatca gcgaggaatt
    24661 cgcccatgaa aagctttctc ccgtccttgc aatgtataag gcaacagata ttcatgatgc
    24721 ttttgataag gcagagcatc ttattgcaga cggcggttac ggtcatacat cttcaatcta
    24781 cctcaatgaa gtaactgagc aggcaaagct cgaagagttc acttcaagaa tgaagacctg
    24841 ccgtatcctt gtaaatactc cttcttcaca cggcggtatc ggtgatcttt acaacttcaa
    24901 gcttgctccg tcgcttaccc tcggctgcgg ctcatggggc ggcaacagtg tcagcgagaa
    24961 cgttggcgta aagcatctta tcaatatcaa gaccgtagct gaaaggagac agaatatgtt
    25021 gtggttcaga gcacctgaaa aggtatatat caagaagggc tgtctcccgg ttgcacttga
    25081 cgagcttaaa aatgtcatgg gcaagaagag agcattcatc gtaacggaca gcttccttta
    25141 tcataacggc tatacaaagc ctatcgcaga caagcttgac gaaatgggta tcgttcatac
    25201 gacgttcttc gacgttgctc cggacccgac acttgccagt gcaaaagagg gcgcagagca
    25261 gatgaaggca ttccagcccg actgcatcat cgcactcggc ggcggttccg caatggacgc
    25321 aggcaaaatc atgtgggttc tctatgagca tccggaagca gacttcatgg atatggcaat
    25381 gcgtttcata gatatccgta agcgtgttta taccttcccg aagatgggcg aaaaggcata
    25441 tttcatcgct gttccgactt cggcaggtac aggctcggag gttactccgt tcgcagttat
    25501 cacagacgaa aagtcaggcg ttaagtatcc gcttgccgac tatcagctcc tcccgaacat
    25561 ggcgattatc gaccctgatt tccacatgag cgcaccgaaa ggacttactg ccgcttcagg
    25621 tatcgacgct gttacacatg ctctcgaggc ttacgcttca atgatggcaa cagactatac
    25681 cgacggcctt gcaattcagg ctctcaaggg aatctttgag tatctgcccc gtgcatatga
    25741 taacggtcag accgatatcg aggctcgtga gaagatggca aactgcgcaa caatggcagg
    25801 tatggctttc gctaacgcat tcctcggagt atgccactca atggctcata agctcggtgc
    25861 attccaccat ctgccgcacg gtatcgcaaa cgcgcttatg attgaagagg ttctccgctt
    25921 taacgcagca gaggctccgg caaagatggg tacattcgca cagtatgacc atccgcatac
    25981 acttgcacgt tatgctgagg tttcaagagc actcggcttc ggcggcaaga atgataagga
    26041 aagtcttgag aaccttatca acgctatcaa tgcacttaaa gagagagtag gcatcaagaa
    26101 gacgatccgt gattacggcg ttgacgagaa ggacttcctc gatcgtcttg acgcaatgac
    26161 tgagcaggct ttcgacgacc agtgcacagg tgctaacccg cgttatccgc tcatgagtga
    26221 gatcaagcag atgtatctcg acgcttacta tggcaacaag catttcacag aagaagaaaa
    26281 acccaccgct gccgattttg aggctacgga cgacaagcat gatttcaaaa aggcttatcg
    26341 cagagccgaa aacggcaaaa gcaaagagta aggaggctta tatatgaatt tggacagaat
    26401 tattgcggtc agaaccgata agaccgtcta ccgtgacggc gaccgctgcc ttaaggtttt
    26461 caatgaggac ttctcaaagg ccgatgttct gaacgaggct ctcaatcagg cccgcgttga
    26521 ggaaacagga cttaatatcc cgaagatcct tgaggtcact gttattgacg gcaaatggac
    26581 tattgtttcc gaatttatca agggcaagac tcttgcacgc ttgatggaag aaaacccgga
    26641 gaaaaaggac gagtatctca gcctttttgt tgaccttcag ctccagatgc acagcaagac
    26701 ctgcaagggc ttgacaaagc tcaaggacaa gatgaacaga aagatcagcg agaccgacct
    26761 ctcggcaacc gttcgctatg accttcacac acgtcttgag tccatgccta agcacaacaa
    26821 ggtttgccac ggcgatttta acccgacaaa cattatcatc acagacgacg gcactccgta
    26881 tattctcgac tggtctcatg ccactcaggg taacgcttcg gccgacgcag caagaaccta
    26941 ccttcttttc tggctcaacg gtgatatcga cggcgcaaag aagtatcttg accttttctg
    27001 cgagagaagc aacaccgcta agcagtatgt tcagaagtgg atgccgatcg tagcggcttc
    27061 acagacagtt aagggcaatg aggcagagcg tgagttcctg cactcttgga ttgacgttgt
    27121 tgactacgaa taattaaggt aacatcgcac aaattgatcc gcacgtctca attgtgcggt
    27181 attaccttac acaaattgac ttaaaatgac cgccgccgat cggttccgat aagcaagcac
    27241 gaatcggtcg gcggatacct aagataatta acttaaaaat gggggatatt tcaatgaaga
    27301 ttacggtttg tatcggctca tcatgccaca tcaaaggctc acgtcaggta gttgagcagc
    27361 tccagtatct tattcacgaa aacaatctcg gcgacaaggt cgagcttggc ggaacattct
    27421 gcatgggcaa gtgccagcag ggcgtttgcg ttaccgttga tgacgagttc ttctcagtct
    27481 caccggagac ggtaaaggat ttctttaacg aaaacgttct tgcaaaggtg tgaaaataaa
    27541 tgatcgttca aatctgtgta ggttcatcct gccatttgaa agggtctgaa aaattggtag
    27601 agctttttca aaaggctatt gccgagaata agcttgacag cgaggttaca cttgccggca
    27661 gtttctgtac cggcaagtgc aatcgtgtcg gtgtgaccgt taccgtggat gacgatgttt
    27721 tcacaggtgt tacacccgat aatttcagca gctttttcaa tgataaggta cttaacaaaa
    27781 taaacgagga aaggaaataa cggaatggaa tgtctgactc tgaaaaaatc caactgtaag
    27841 aattgttaca agtgtattcg tcactgtccg gtcaaatcaa ttcgtttttc cggtaatcag
    27901 gcatatataa tcggcaacga gtgtattatg tgcggtcagt gcttcgtggt ctgtccgcag
    27961 gacgctaagc agatcgttga cgaaacagag aaggtcaagg tgcttttgca gagcggtgac
    28021 cccgttgttg tcagccttgc tccgtctttc atagctaact atgagggcgt gggtatcggc
    28081 gcaatgcgtg aggctctcaa gcagctcggc ttctacgacg tcgaggaaac agccatcggc
    28141 gcgactatag ttaaaaatga atatgagaga atgatagggg agaagagcca cgatataatc
    28201 atctcttcct gctgccattc cataaatctt cttatacaaa aatattttcc ggcggaatta
    28261 ccctatcttg cggatgttct ttctcctatg caggcacact gcaaggatat caaaaagaga
    28321 atcccgaatg caaaaacagt ctttatcgga ccgtgcgttg ccaagaagga cgaagctcag
    28381 tattatgagg gtatcgttga tgccgttctg acctttgacg agcttacaaa ctggctcaaa
    28441 gctcagaata tagagcttaa gccggaaatg gatcaggacg aaaacagccg tgcaagattt
    28501 ttcccgacga caggcggaat cctcaagaca atgaataccg agaacacggg ctacacatat
    28561 atggctattg acggaacgga gaattgtatc gccgctctgc gtgatattga aagaggtgga
    28621 cttcatagct gctttatcga aatgtcagct tgtgtaggca gttgtgtcgg cggtcccgtt
    28681 atggaaaaat tccaccgttc acctgtcaag ga
//
LOCUS       DAWBSW010000005        25990 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_400_length_25990_cov_3.164642, whole genome shotgun sequence.
ACCESSION   DAWBSW010000005 DAWBSW010000000
VERSION     DAWBSW010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 25990)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25990)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25990
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_25990_cov_3.164642"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9095..9104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13950..13959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7311 a         5795 c         6207 g         6657 t
ORIGIN      
        1 gattttgatt gccccagtaa ggaaattctt gatattgtaa ccaagcattt tgaaggacag
       61 aaaaaatcta tgacggctct tgaggaggcc gagcttgacg ttatatttga aattgtaacg
      121 caggaaaaca gcgttgaaag cttcgacgct tactttgcaa cggcgaacga gccgaagaag
      181 gaagaaaaga aagagaaaaa gactgaaaaa tcagacgagc cgaagaagtc ggaagcaaag
      241 tccgatgctc ctgccaagga cgagaaaaag tctccggcaa agagcaacaa tcagccgaag
      301 aaaaaacaga aggatcccaa cgcagctccg cagagcagaa caaagggcga attcagaacg
      361 atcgacacaa gggcaagcaa tgttgatctt gataaataca acgagaagta cgagaatatc
      421 tctcctgtca atcaggacag aagaagcgac agaacggtca acaagcagaa gctcaagcag
      481 aagtctcagc agcgcagacc gggcatgaaa tcacgccgtg agactgaggc tgagagactc
      541 aagagaattg cggcggagag agcaaagaag ccgcagcttc acattcaggt tcccgaggag
      601 ataacagtcg gcgagcttgc cgcgcttctc cacatgacgg cggcagaggt tatcaaaaag
      661 ctctttgccc tcggtcagat ggcaacggtc aatgaggtca tcgactatga tacggccgaa
      721 atcgttgcga ccgagcttgg tgcaaagtgc gaaaaggcag ttgttgtcac aattgaggat
      781 agaattattg acgacagcga ggatgatgat aaagatcttg ttccccgtga ccccgttgtt
      841 tgcgtaatgg gtcacgttga tcacggtaaa acatcgctcc ttgacgcaat cagacatacc
      901 tccgtcacct ctaccgaggc aggcggaatt acacagcata taggcgctta cagagttgat
      961 ctcaacggtc agaaaatcac attccttgac actccgggtc acgccgcatt tacatcaatg
     1021 cgtgcaagag gtgcccaggc aacggatata gctgttcttg ttgttgctgc agatgacggt
     1081 attatgccgc agacgattga ggctatcaac catgcaaagg cagcggaggt tcagataata
     1141 gttgccatca acaagatgga cagaccgggt gcaaatcccg acagaatcat gcagcagctt
     1201 acagagcaca gcctcgttcc cgaggagtgg ggcggcgacg ttatctgcgt tcctgtttcg
     1261 gcaaagacac atatgggtat tgataagctg cttgaaagca ttctccttgt tgccgagatg
     1321 caggagctca aggctaatcc gaacagagcg gcaaagggca tcgttatcga agctcgtctt
     1381 gataagggca gaggcccgat cgcaaccctc cttgttcaga aaggtacgct taattcaggc
     1441 gacatcatcg ttgccggtca gtctgtcggc cgtgtccgcg taatggttga cgacaagggc
     1501 agaaagatca aatctgcagg cccgtccgtt ccggttgaaa tcacaggtct tgcagatgtt
     1561 ccgaattcgg gcgatatctt tgacgcagta acagatgagc gtcttgcgcg tgagcttgtt
     1621 gagcagagaa aggctgaggc gaaggaagag cagttcaagt ctgttcaaaa ggttactctt
     1681 gataatctct ttgattcgct caaggacggt gagatgaagg agcttaacat aatcgttaag
     1741 gctgacgttc agggctcggc tgaggctgtt aaacagagcc ttgaaaagct ctcaaacgag
     1801 gaagtcaggg ttcgtgttat tcacagcggt gtcggtgctc cgagcgagtc tgacgttatg
     1861 cttgcaaacg cttccggcgc gattatcgtc ggtttcaatg ttcgtccgga tccggttgct
     1921 ataaaccttg cagagcgtga cggcgttgag atgaggatgt acagaatcat ctacgactgc
     1981 atcgaggaaa ttcaggcggc tatcaagggt atgcttgctc cgaaattccg tgaggttgag
     2041 cagggccgtg ccgaggtcag aaatgtcatt actatttcct ccgtcggcaa gatcgcaggc
     2101 tgctatgttc ttgacggaaa gattacaaga aattcaaagg ttcgtgttgt ccgtgacgga
     2161 atcgtacttg ctgttgacga tatcgcttcg ctgcgccgct tcaaggacga tgtgaaggag
     2221 gtcgcttcaa actatgagtg cggtatcggt ctcacaaagt tctccgatat taaggagggc
     2281 gacatctttg aagcctacac tgtcgaagaa tacagagatt aagaggtagc ttatggcagg
     2341 ttacagaata gacagagtat ccgaggatat taagcgtgaa ataattgcgg ttatcagaga
     2401 acttaaggat ccgagggtaa tggataaaat gcttaccgtg gtgagggtgg aggtcagctc
     2461 cgacgcctcc tacgctaagg tttatatcag cgcaatggag cgacttgacg ttgctaagga
     2521 ggcagtcaag ggtctcaaga gtgcgacggg atatattcga agagaggtcg gcaagcggct
     2581 tcatttgaga aaaactcccg agcttagctt tgtagctgac gattctattg agcacggtat
     2641 gaatatcgtt aagattatgg atgatttaag gactgaggac taatggttat caatcttgaa
     2701 acggcggttg aatttttaaa aacaagggac aattttttga tattgaccca ttcacacccg
     2761 gacggagata cgctcggttc ggcttttgcg ctcaaatatg cgcttgaagc cgtcggcaaa
     2821 agggcgaacg taaagtgcaa tgacacggtt gcgaaaaaat tcgattatct tggaaaagtt
     2881 tgcgaggatg agtgcgcttt tgacgctctt gttgcggttg atgtggctga cacaaagctg
     2941 ctcggcaagg actttgagga gaaattcgcc gacaggattg agctttgtat tgatcaccac
     3001 ggttcaaacc gtatgtttgc cgaaagaaca ttgcttgatg cgaatgctgc ggcggcatgc
     3061 gaaatagttc ttgaagttat tcatgcgctc ggcattaagc cgacgaagca gattgccgat
     3121 tgtatataca cgggaatttc aaccgataca ggctgcttcc gctattcgaa cgtcacccca
     3181 cgcacaatgc gtatggcggc ggaaatgatt gaatgcggcg ctgacaatgt gacgataaat
     3241 acggttatgt ttgaaacgaa aacgagaaca tacgttgcgc ttgaaaagct ggccgtcgga
     3301 ggaatgaaga tgttccttga cggtaaatgc gcgctgataa cggttactca ggatatgtac
     3361 cgtcagagcg gctcggacga gagtgaggtt gacgcaatcg cttcgcttcc gaggcagatt
     3421 gagggcgtat tggtcggtgt tacaatgcgt gaacgcaagg acggaagcta taagatctca
     3481 atgagaagta accgaccgat caacgtttcg gaaatctgcg cggcaatggg cggcggcgga
     3541 cacccgcagg cggccggctg tcaggtcagc ggtacacttg aagaagcaac tcagaccgtg
     3601 atagaaaatg ttaaaaaata tatagaaaaa ttataaaaga aaaacgcaaa cccgattgat
     3661 ttgggcttgc gttttttagc ttttaacaaa ttaaaaaaca accgtcagaa gcattttaaa
     3721 tttatcctca ccgaaaacgg agtggggatg cttggcgggc ataacgattg tgtcgccctc
     3781 gtgcaagaaa tattcaatgc cgtcaatagt gatttttcct gttccgtcaa ggcatgtaac
     3841 cattgcgtcg ccgcccgact catgggtgct gatttcttcg cccttgtcaa aggcaaaaag
     3901 tgtaacactc agtgcggagt tttgcgcaag agttttgctc actacctgac ctgtctgata
     3961 ttcaacctgt tctttcaggc tgaggatttc agctttattt atgtttttat aaccgttcat
     4021 aactcagtcc tcctgctcgg ttatatcatt gcccgtttag taactgaaaa ttctttttgt
     4081 ttgaggaata aagctttttg aacacctcaa agtttttgtc ataaactttt ttgtctgccg
     4141 tgtttgcatg gaaggtcttt ttgaccggta tataattctt aacactgccg agatccttaa
     4201 taattccgag accgattcct attatagccg ccgcaccgac agcaccgaca tcctgcggct
     4261 tctcaacaac ttcaatcgtc ctgccggtta tatctgcaag aatctgtgcc gttacatcgg
     4321 agagcgcacc tccaccgaca aagcggattg tctgtgaggt ctttatcttt ctgtcctgcg
     4381 cctcaagcat ccagcgcaga tgatagcaaa cgccctcgat aaccgccctg agcatatcgg
     4441 atttacctgt gttgatgttg atgttaaaga acattcccgc ggcattcgga tcctcaaacg
     4501 gacagcggtt accgtgaagc cacggagtga aaataactcc gtttgcgcct gccggaacag
     4561 agtcgataac ctctgtcata tagtcgtaaa ggctggtgta cgctttttct gtacggtctg
     4621 ttatcttctt cttttcaatg aaaatcccta tttcatcaag acaaagatga tccttgaccc
     4681 attcgaggca tttgcctgcc gtttccatct cggcaaaata gttaaagctg ttcggatcag
     4741 caccgacaat tgcggcaatc atcgcagagg tatcgacaag ttgtttttct acgactgtac
     4801 cgacccagcc cgatgttccc gaataaatgt gagtatcgcc gacggagcag ctgcctgcgc
     4861 cgacgccgat gagcgaggcg tcaccgccgc cgccgaatac gggtgttccc ggcaaaagac
     4921 cgagcttctc cgccgccttt tcggtaactc cgccgaccat gtcggtcgat tcgattatat
     4981 caggcagatg attcatgttt acatcaaaaa tatcggcaac ggttttgctc cagccctctt
     5041 tgccctttct tgtatcataa aggagagtgg caaacgccga atcctttgtc atcgtgaatt
     5101 tatccgtaca acggctgata aggaaatcct taacatcaag ccatttgtac gcttttttgt
     5161 agttctgcgg ctcgttgttt tcaacccact tgtatttgta aacggggtcc tttacgctgc
     5221 atgagacggc tcccgtgacg gcaagagatt tgagaagcgg aacaatattt gctcctgcaa
     5281 tctgtaagcc gtatgccatg tattttttca gctcctcctt ggcgcgctga tccatatagc
     5341 tcatgggtct gcgtacggga tcaccgttct tgtcaacgag gacgagagcc tgcatctgag
     5401 agcagaatga aattcccgcg atttgttcgg gcttgatatc cgtctttgta aagatttctt
     5461 tcgtggtggt acacattgcg ttccaccatt cctcggcgtc ctgctcggcg ccgccgtttt
     5521 cgagaatata aaggttgtat gagccgtaag aattgacaat cggtttgatt tttttatcaa
     5581 tctcaaacag gcaggtttta agtcctgttg ttccgacatc atatgcaata acgtaagcgc
     5641 tcatgttcaa cactccagtc aggttaaatt tctgtctcta ttctatcata aagaagatca
     5701 aaagtaaaaa tattttatga atttttacaa atttttttta gtgacgtatt taaaaactaa
     5761 actatatgtg ttaaaataaa tctaattaaa aaagcaactg cccgtgaagt tatacgtttt
     5821 tcggcagttc ccaaaggagg tttttttatg ggaaacaatt ttgctatcaa aaattatctt
     5881 gatgccgata aggtcacaaa ggagcttgac gctcttatta aaaagcctat ttacagcatt
     5941 aagccggaag ctctcaaaaa atatgagaga gaatattacg caaagaaatg tgcaaagtca
     6001 aaggaaatga tcgacattgc caagcagaga atacccggcg gcgttcagca taacctcgca
     6061 tttaattatc ctttcccgct tgtttttacc aaagcatcgg gtaacaagct ttacgatata
     6121 gacggcaacg aatattacga ttttctccag gcaggcggac cgacgattat cggcagcaac
     6181 cctcccgagg ttcgcgaaaa ggttattgaa ctgctcaatg actgcggccc gtcaacaggt
     6241 cttttccatg agtatgagta caagctcgca aataaaattg ccgagtgtgt tccgtctgtt
     6301 gaaatgttcc gtatgctcgg ttcgggcaca gaggcttgta tgtgtgcggt tcgtgttgca
     6361 agacttgcga caaagcacaa gaatatcctt aaaatgggcg gcgcatacca cggctggtcg
     6421 gatcagcttg cctacggaat gagagttccg ggcacaaaga acctccagtc aaggggcgtt
     6481 ccgatgtttg tattcaagca cacggacgag tttttcccga atgacctcaa tgaccttgaa
     6541 agaaagctca agaagaatga gcttacgggc ggcacggcag gcgtattcat tgagcctgtc
     6601 ggtcccgaga gcggaacaag accgttgagc aaggaattta taaagggagc agaggcactc
     6661 tgccataaat acggcgcact tcttatattc gacgaggttg tcacaggctt cagaatcgga
     6721 ctttccggtg ctcagggcta tttcggagtt gatcccgatc ttacaatttt cggcaaaatt
     6781 gttgccggcg gatatcccgg agcaggcgga atcggcggac acagagatgt tatggctcac
     6841 ctcggcgcag gactcgactc ttcgggcaag aaggttaaaa aggcaatgtg cggcggcaca
     6901 atggcggcaa ctccgctttc gtgcgttgca ggttatcata caatttgcga gattgaaaga
     6961 accaatgcct gtgaaaaggc aggacttatg ggcgacaggc tcatcgaggg tattcagaag
     7021 tcgatcaaga agcataatct cccgttcgtt gcgttcaatc agggctcaat ctgccacctt
     7081 gatacagtag gcacaatgca ctttgcaatc aactggtcca agccgtggga gattccggaa
     7141 attctcaagc agacgagcgc ccgtcagaag gaaatggagc acatgggagc cgcttacatg
     7201 gcagaaggta tggttactct cgccggcagc agactttata ccagtgctgc atatacggaa
     7261 gaagatatcg acgatgcaat tgctcgtttc gacaaagtat ttgacaactg cggcagaatc
     7321 ggctgacaga ataaggggga cgtttaggtg tacactttgg aagaaggaaa aaaggctgtt
     7381 atcgaagcgg gtcacagact gcttgaaaca ggcctcatag cgagaacgtg gggaaacgta
     7441 agcgcaagaa tttcggacac tcagttcgtt atcactccga gcggcagaac gtatgatacg
     7501 ctcacgcccg atgatattgt tgttgtcaat attgacgatt gcagctatga gggcgacatc
     7561 aagccctcaa gtgaaaaagg catccatgcc gatgcttacg ctttgagaag cgacgttaat
     7621 tttgttatac atactcatca ggtcaacgcc tcggttgtcg gagcggccgg cattgacgtt
     7681 gatccggtgc caaaggctta cagagatatt ctcggcgaga ctgttccttg cgcaggctac
     7741 ggtatgccgt caacggataa actgagaaac acagtaaaaa aggaatatga aaacaatccg
     7801 ggctgcaatg cttttcttat ggcacaccac ggtgcggttt gcctcggcaa ggattttgat
     7861 gagacctttg aaatcattca ggctcttgaa aaggcatgcg gaagtaagct tgaaaaggca
     7921 tttctcatgt atgagggcac cgctgaatat acaaagaaag cgatgctcaa cagctatatc
     7981 agacacacgg gcggcaaggc tctgcccgag tatgtgtcgg atttcggaag cagtgtcaga
     8041 gacggtgagt ttttcctgct gacctttaag agcggcgcaa gcttcaatgt aaaaatcaag
     8101 ggctgcacct gcgacagtga cgcaaagctg cctgcaattg cgaaaattca tgctgcgatt
     8161 tataaatcgt ccgatgtaaa ttatattcgt cacctttccg aaagcgacat cattgcatac
     8221 agcatgacct cggactgtat taacgcaaga cttgacgatt ttgctcagat tgcaggtgca
     8281 aaaatcggct cgttccgctg ggacggctcg gcaagctgcg cttcggaagc ggcaagaaaa
     8341 attaagggta aaaatgcagt gcttattcgc ggctcgggtg ctattgtcac gggtaaaacc
     8401 ataggagatc ttgacgcagt tgagcttgtt atgagcaagg aatgtaaaac ccagataggt
     8461 tcacttttcc tcggaagcgg cgagcctctc ggctatgccg accgtgttct tcaaagagta
     8521 atttatgtaa acaagtattc aaagaaagca gagtcttaaa acaagcaaaa agccgtgaat
     8581 cacaaagcat tttcgcaatg tggtttacgg ttttgttttt ataaattatt tcctctcgga
     8641 taaaaatttt tcagtctcgg ccggacttca aattgatttc ggctcagaca aattataaca
     8701 taaggattct cttttacaat tataaagggc gaaaaaacaa attgtatatt aaaattgtta
     8761 ataaaatgtc aatttaacca atattttcaa gagtagggat taaattattc atatactttt
     8821 ttcattgact tatttgccga aatactttat aatttcatag gattttacaa tgagggagaa
     8881 ttggttttta tgttatcgaa tacagaaaag actatggaca agattgttgc cctttgcaaa
     8941 aacagaggat tcatttttgc cggcagcgat atttacggcg gtcttgcaaa cacatgggat
     9001 tacggcccgc tcggagcaag acttaaaaac aatgttaagg acacctggag aaagcgtttt
     9061 atccaggaga gaaagaacag ctacgaggtt gacgnnnnnn nnnnttgacg ccgatattct
     9121 catgcatccg agagtatggg aagcaagcgg tcacgttgcc agcttctcag atccgcttct
     9181 tgactgcaag gaatgtaaaa tgcgtcacag agcagaccag cttatcgaca actttgatcc
     9241 ggaagctcat gcagacggca tgacgaagga ggagatgttt gattacatca aggctcattc
     9301 gattccgtgt ccgaactgcg gcaagcataa ctttacggac atcagagaat tcaatcttat
     9361 gttccagaca tacaggggag ttactaacga tgcaaagagc attatttatc ttcgtcccga
     9421 aaatgcgcag ggcgaatatg ttaactacct gaatgtacag cgcagcatga gagctaagct
     9481 cccgttcagc ataggtcaga taggtaaggc tttcagaaat gagattactc ccggcaactt
     9541 tacattcaga accatagagt tcgagcagat ggagttccag accttctgca aggagggaat
     9601 ggacacagac ctctacgaat atttcaagaa ttacggccat cggtattaca tggatctcgg
     9661 tattgccgag gagcatctcc gcttccacga ccatgagaag cttgctcact atgcaaaggc
     9721 ggcgtgcgat atcgaatttc tcttcccgtt cggatggggc gagatcaacg gtacacacaa
     9781 cagaacgaac tttgacctca caagacatca ggaatacagc ggccagaagc tcgaatacct
     9841 tgatcccgag acaaacgaaa gatatatacc ttttataata gaatcaacat acggactgga
     9901 cagaacggtt ctcgcccttc tctgcgaggc atatgatgag gaagttattg atgccgagaa
     9961 gaacgataca agagttgttc ttcacctcaa tccggcaatc gctccttaca aggctgcggt
    10021 tcttcctctc tcaaagaagc tttcctcgga cgctctcaag gtttatgaaa agcttcagaa
    10081 ggatttcatg gttgactttg atgagacagg ctcgatcggc aagagatacc gccgtgagga
    10141 cgagatcgga actccgttct gtatcacatt cgactttgat tcccttgagg acaactgcgt
    10201 aaccgttcgc gacagagaca caatggagca ggttcgtatg ccgattgatg aaatcagtga
    10261 atatatttcg tcaaagatta cattctaatt attggcggat aataaacata aacagaattt
    10321 tgaattggaa ggcaaagcta tgaaaaaaat caattttaca gaaacagttc tccgtgacgc
    10381 tcagcagtcg ctgattgcaa cgagaatgcc ttttgaaacc tttgagccga ttctttcaac
    10441 aatggacaag gcgggttact actcgctgga atgctggggc ggcgcaacat ttgattcgtg
    10501 tctgcgttac ctgaacgagg atccgtggga gagactcaga aagattcgca aggcatgtcc
    10561 gaacacaaag cttcagatgc ttctcagagg tcagaatctt ctcggctaca agcattaccc
    10621 gaacgatgtt gtaagaaagt ttgtagcaaa gagcgttgaa aacggaatcg acatcatcag
    10681 gatttttgat gcactcaacg atgtaagaaa cctcgaggtt gccgttgacc agacaattaa
    10741 gtcgggcgct cacgcatcgg gtactatttg ctacacgctc agtccggtgc acaacacaaa
    10801 ggcttatatc aagcttgcca aggatatgga aagcatgggc gttcagtcca tctgtatcaa
    10861 ggatatggca ggtatcatca gccctaagga ggcttatgag cttgtagatg cactcaagga
    10921 gaacgttaaa ctcccgatca tccttcacac gcactgcaca acaggcctcg gcccgatgac
    10981 cttgctcaag gcggccgaag cgggcgttga tgttatcgac accgcaattt caagcttctc
    11041 gggcggcaca agccagccgg caacagaaac ccttaactat atccttaccc aggacggtta
    11101 taagacaggt cttgataccg atgttctcaa gcagatcaac gatttcttca agcccgttaa
    11161 ggcggatgcg ctcaagagcg gactgctcaa tcctgttgtt ctttcgacgg atacggacgc
    11221 tctgacatat caggttccgg gcggaatgct ttcgaacctt gttgctcagc ttacggctca
    11281 gaagaagatg gataagtttg aagaggttct tgaggaaact ccgagagtaa gaaaagacct
    11341 cggttatccg cctcttgtta ctccgatgag ccagatggtc ggtgttcagg caacgtcgaa
    11401 tgtccttgca ggcgagagat ataagaatgt ttccaaggag attcgcgctt atgtaagagg
    11461 cgagtacggc aaggctcccg gaaagataga tcctgcactc atcaaaaagg ttctcggtga
    11521 cgagaagccc atcaagggca gatatgcaga taccctcggt gacggttacg aggctgcaaa
    11581 ggctgagatc ggcgcaaagg cacacagcga agaggatgtt ctttcatata tcgcattccc
    11641 cactcaggcc gaagcgttct tcgacaagcg tgacgaaaga gcgaagaaca ccttcaaata
    11701 caccattgaa aaaattgatt gaggaggaag atgaatatga atatgactcc taagcttctc
    11761 ggcgttttta ctcagatcta cggttcaaac gatgccatgg atttcggtaa agcggcactt
    11821 gttgcggttg tcggatttgt taccgttatc gcaattcttg cgattatcgc actgttcatt
    11881 aaggctattg ccgcaatgtt cgataaaaca agcaagactg cccaagctcc tgttgatccg
    11941 gcacctgccg cacctgcggc tccgagcgta cagcccgcac agtcgggagt tgagcttgta
    12001 ggcgttgacg agcctacggc ggcggttatc atggcaatag taagtcaccg gagcggaata
    12061 agcctggaca gactcgcatt taaatccatt aaattatcgg aggacaaata aatgaaatat
    12121 gttgttacgt taaacggaaa aaagtatgat gtagaggtta acgaaagcga agcagttatc
    12181 acctccgtaa gcaatgccgc tccggcagcg gcacctgttg ctcccgttgc agcggctccg
    12241 gcacctgcgg caagcacagc cccgagcgca ccggctgcgg tagtaggtac ggcagtcagc
    12301 ggaactcaga tcacatcacc gatgcccggt acaattctca agctgaatgt tgccgagggt
    12361 caggctgtta aggcaggcga cgttgttctt attctcgagg ctatgaaaat ggagaacgag
    12421 atcgttgctc cctgcgacgg cacgatcaag cagctccttg tttcaaaggg atcgactgtt
    12481 gacacggatc agattcttgc agttttataa taaaggacgg tattcaagat ggacttaatt
    12541 ttaaatgttt tgaaaggctt gtgggaatcg tccggatttg caactctcga gtggcagaat
    12601 cttgtaatga tactggtttc attcgtgttc cttttccttg ccattcatcc taaattcagg
    12661 tttgaacctc ttctcttggt tcctattgca ttcggtattc ttcttgcaaa ccttcccggc
    12721 gcaggcatca tggatgacct cactgccggt cagatattcg gcggcgacgg ttcgcttgcc
    12781 ggtcagggcg agcactggta tgattccggc gttcttcgaa ttatttatgc cggtgttaaa
    12841 tcaaacatat tcccgtgtct tatctttatg ggcgtaggtg ctatgactga tttcggacct
    12901 cttctttcca atccggtcag cttgcttctc ggcgctgcgg cacagctcgg tatttatgtt
    12961 gcgttcctta ttgctattgc gcttggattt aatccgcagg aagccgcttc aatcgctatt
    13021 atcggcggtg cggacggccc aacggctatt tatcttacgt caaagctggc gtcgcatctt
    13081 cttgcgccta ttgcgcttgc ggcgtactca tatatggctc ttattccgct tattcagcct
    13141 ccgataatga aggctcttac gactaagaaa gagcgcgagg tcaagatgac ccagcttaga
    13201 aaggttacaa agacagagaa gatcatcttc ccggtaatcg ttcttgtcct tgttgcactt
    13261 attcttccgt ctgtcgctcc tctcctcggt atgttcatgc tcggcaacct cttccgtgaa
    13321 tgcggcgttg ttgacagact ttccgatgtt gctcagaatg cgcttaacaa tataattgtt
    13381 attcttctcg gtgttaccgt aggcgcaaca gcaaacggtc agagcttcct tcaggttcag
    13441 acactcaaaa ttgttgctct cggacttatt gcattctgct tctcaacagt cggcggtgtt
    13501 ctcctcggca agcttctcta tctcgttacg ggcggaaaaa tcaatccgct catcggtgct
    13561 gccggtgttt ccgctgttcc tatggcggct cgtgttgctc agaacgaagg cagaaaatac
    13621 aatccgacaa atttccttct catgcatgcc atgggtccga atgttgccgg agttatcggt
    13681 tcagccgttg cggcaggttt cctgctcatc atgttccagt gatttatcaa aggacaggtt
    13741 cattctgtga gcctgtcttt tttcaatatt tgaaagataa caaattattt aaaaaaaggc
    13801 ttgatttatt tttaaatttg cgatagaata agacaattta tattctgagt ggaggttgta
    13861 aaatgacttt tgatgaaaaa ttcggaaaag aaggtttgac ctttgacgat gttctgctca
    13921 ttcctgccga atcggatata cttccaaagn nnnnnnnnna tatttcgaca aagctttcga
    13981 acacggtcgt tcttaaaaca cctattttga ctgcggctat ggataccgtt accgaagccc
    14041 ccatggcaat tgcgatagcc cgtgaaggcg gcatcggtgt tattcataag aatatgccga
    14101 tggaactcca ggcacaggag gttgacaagg taaagcgttc tgaaaacggc gttattgctc
    14161 agccgttcag ccttactccc gatcactatg catatgatgc ggaaaatctc tgcaagaagt
    14221 ataaaatttc cggtgttccg atttgcgacg aaaaaggaaa actcgtcgga attctcacaa
    14281 accgtgatat gcgttttatg actgatttca atatcaaaat cagtgaggta atgacaaagg
    14341 atcaccttgt taccgctcct gtcggaacaa cgctggagca ggcaaagcag atccttatga
    14401 agcacagaat tgaaaagctt ctgcttgttg acgatgaggg ttacctcaag ggtcttatta
    14461 caataaaaga tattgaaaag agcgttcagt atcccaattc ggcacgtgac aatggcggca
    14521 gacttctctg cggtgcggct atcggtgcaa cgtcagatgt gcttgacaga gcaggtattc
    14581 tcgcaaaggc cggggttgat atgttcgttc ttgactccgc tcacggtcac agcaagaata
    14641 ttctcaaagc ggttgaaaag gttaagaccg cattcccgaa cattacgctt tgtgcaggta
    14701 acgttgcaac ggccgaggct acagaggctc ttattgctgc aggtgccgat gttgttaagg
    14761 tcggtatcgg acccggttca atctgcacga cacgagttgt cgcaggtatc ggcgttccgc
    14821 agatcacggc aatctttgat tccgcgaatg ctgccgcaaa gcacggtatc cccgtaatcg
    14881 cagacggcgg tatcaagtat tccggtgaaa ttgttaaggc tatcgccgca ggcgcaagcg
    14941 caataatggt aggttcactc gttgcaggct gcaaggaaag cccgggtgca acagaaatat
    15001 tccagggcag aagctttaag gtttatcgcg gaatgggcag ccttgccgcc atggcaaacg
    15061 gcagtaagga cagatacttc caggaggaca acaaaaagct tgttccggag ggagttgagg
    15121 gacgtgttcc ttacaaggga cctctcggag atacaatctt ccagatgctc ggcggtttgc
    15181 gttcgggtat gggctactgc ggctgtcata atatcgaaga gctcaagaca aagacaagat
    15241 tcgtcagaat aacgaatgcc ggtcttatcg agagccaccc ccacgacgta tttattacaa
    15301 aggaagcacc gaactattcg ggcacgaaat aattataatt tgtcgaaacg gcaggactta
    15361 tgttatgttt tataacgtat gtcctgccat cgttttttgc atatataatt cataaagaga
    15421 atgatacaaa atttcattat tacttatgta taaaatgtac aaaatcaata atacgactaa
    15481 aaaaatattg acttttgacg ttttatgcgt tatagttaaa ctaaccataa aagtggtgtc
    15541 attgggtgaa ttgcgccttt tgcatgaagc cctcattacg acccacctgg tttaccaatg
    15601 cgtcgtaatg acgttgatgt ttgactcatt taaacggctg tatgcccttg cgggaaggct
    15661 tcggcaacag agtctcgttt ttaaaagagc agcaatgacg taatgatgtc gcgccggtat
    15721 gcacgagccg gaaaaaacgt gcggtatgta tgctaaaggc atataaataa tatggaggtg
    15781 caatccaaaa atgagtaatg caaaaagatt cctcagtgtc attcttgcaa tgatcatggt
    15841 tctttcaacg ctcgttgtcg gtgcaagtgc ttattctgct tataaggacg aagctatcaa
    15901 ggatcagtac aataagcttg ataagccggt tcttacaaca gaacagtatg catcagcagc
    15961 agttgatgag cttgaccgta tgctcggtaa ggagcagatc aaacttgaaa agtctgatct
    16021 ttacggtatc ggcgaacttg atcttacatc agttgacaaa acgatggagt cggtttacaa
    16081 cttcgtaacc ggccgaacct ttgaaacatt caaaggaatg cttggcgacc tcgccaacct
    16141 caatgctaac gcttttgcta cagtcagaag atcgagcgga gatatcaacg ttctttacgc
    16201 tgttctccag ttcctctatg acaataaggg cattttcgtc agctacatta accagacgat
    16261 taacctcggc acaattcttc cctcggttgt tgacctcagc gattacactg atgttaacca
    16321 gcttctcaag aagatgctct acgaggtagt ttacacaact aaggattctg ccggcaacaa
    16381 gattaagcct acagttccta agacggtaac tgagtctgtt gattcaatgg ttcagaagct
    16441 tattgataat cttatagctc agaatcttcc cgagctcaac ggttatacaa acatcagctc
    16501 aggaacaacc tacacattca ttgacaacgc tctcaagaag gtattcaatt cttcccttgt
    16561 tcttgacgcc ctcaacggca gcgttaagga tgagatcaag aaggcttgcg gcgtagagtt
    16621 ccagaagaat gctgacggtt cctacgttaa ggatgctaac ggaaacaagg ttgaagctaa
    16681 cagagatcat cttaacagct atgcaaagta tctcaatatc gactatacat atagcggatt
    16741 tactttcaca agcgcttcgt tcatgtctca gctcaacaac attctcggtt cattcattaa
    16801 ccttcttctc aagcccggcg tttacacctg gaagacaggc gataactccg ttcttatgga
    16861 taaccttgta ggtcttgcaa aggctgttct tatcaacacg ggcgatgact tcttcgcttc
    16921 ttacatcaag gttgctacac ctgcagaagt tgagaagatg tcaaatgaag agcttatcgt
    16981 ttatgctctc cgtgctatca tcaacggttc tgttaagggt atgtacattc ctgatgacgt
    17041 aacaacaatt gttgatttcg cttactacgg aatgactcag cttcttgcaa catctgctcc
    17101 ggagcttgat ttctccggct atgcacacac aatcgataca atcatcgtta tgggcgttaa
    17161 ctacgctatc tacagcgtaa attccgctat cgacatgggt cttacctatg tttccgatat
    17221 gaacggtgtt gaccagcagc ttaaggttgc agctcagtac ggtatcgaaa attacggcgg
    17281 acttctcaac ggtctttcac tcagcacgag tgacagcggt tggacaactc tcaacaagat
    17341 tgttgacggc cttttcggtc tcaactggct tccgacatct gcaaacaagg atgtcaagac
    17401 atttgtaatc gacaacctta tcaagaagat tgctaacctt gatctcaagg gcgttcttga
    17461 tatgtttgag ctttctgcat tcccgtcagc ttccgatctc aacaagactc cgaaggctgc
    17521 agttctcgac gtagttacaa gaattgttaa catcatcttc ccgggtgctt tcaatacttc
    17581 agctacaact ttcgatgcac ttgtaaccaa cgcagctctt gcaaacacag ttgatgcgat
    17641 cttctcggat ctttacaact acagagtaaa ccttgttaag ggtgctcttc cgatcgtatg
    17701 ttcggttctc agccttacaa cagctcagga atttaagttc ccgaccgtta cttgtgataa
    17761 attcatttat gacaagacag gttctccgag cttcacgatc aagcttcgta acaactcttc
    17821 aggtatcaat acaggttata caacagcttc cggcgcattt gttcaggata agctctatac
    17881 atatgagatt ctttccatca cttcaaacag ctccgttctt aagcttacac ctgataccac
    17941 aaaggttgct gcaggccagg aggcaaacat cagcgttgcc gctacaagca catattcagg
    18001 ttctaacccg ttcatcgttg ccattacata taacgttttg actgaggacg gatcaaacct
    18061 tacaacttct ccgattactg agtatgttta cagcttcatc tcggctgcag ccgatgacga
    18121 cagcggcaag gaagtcagct atacaaaggg caactaccac atcacgggcg gcccggctta
    18181 catctatgca agcagcatga gagaccttta cagagctcag tacacattcg aaaacacaac
    18241 ggcaaatgaa gttaagggcg catcggttgt tgctaattca tcagctccga tcaaagcttc
    18301 atacatcgcc gttaaggatg agaagtttga tattctcggc aagacaggcg accagaacgg
    18361 tatcgctaag tcatatatct ttgaaacaac cgctgaatac gatgctctta ctcccgaaga
    18421 gaaagaggct gcttggacag caatctgcaa ggttggtaca atgacctcta agcagtacac
    18481 ctacagcatt ggtgctaagt ttggttcgac aacggcatca acagcagtta aggtattctg
    18541 ctatgaggag ttcggacttg gttcactcct ttcttcggaa atcggcagac accgtcaggc
    18601 ttccaactac agcgcaggct ggtcagattg ggagaaggca atgaaggacg ccgttgacgc
    18661 agtttactca ccgtttgttc agggcgcttt cagaaacaca aaggctaaga agtatcagtc
    18721 agcttatact gctcttaagg cagcggttga aactctcgat gctaacgaga tggcaggcgg
    18781 acttgattcc acaaaggcaa ttatgaattc ttatgctccg tcaaacgagg gcaagaatta
    18841 tacagatgct gattacagct tcttcggtgt tgcggactat gagccgtaca catattacaa
    18901 ctaccgtaac gaggttaagc aggctaacag catgattaac agagcagaga ttcccgatgc
    18961 acagggcaag acctatcctg cggattcact cactgttact tacagaaacc atcgtctcaa
    19021 cctttacggc agccgtcttc ttaagaagga cgctttgaag actcaccttg cttatgagat
    19081 tgcaaatgct caggcaaagg gatacaactc ggctgattat actgctgagt catgggcagc
    19141 atatcagaca gctcttaact ttgctaattc tgtaaacaac gattcttcat cgtctctcag
    19201 acagacaaag gttaacacag cttatgagat gctccttgag tatcagaaga gacttatctc
    19261 agcaggcggt tcaacaccgg taacgccgac ttattcgctt atcagcgatg ttgagactat
    19321 tgagatggca gacggaaacg tacttgtcgg tgttttgggc gacggttcta atgatgcaac
    19381 atactatttc agcacaactg agaattgcac tgttgagttt gtagagaaca atcagggctc
    19441 atattcaaca gatgctaaga ttgttgttaa gaacaatgcc ggtgacatta ttgaaacata
    19501 tattgtatcg gtaacaggtg atgttaacgg tgacggcgct tgcggtgacc cggttgacgc
    19561 acttgaggtt gaagttgtct ctaacggttt ggatgacttt gcaacatcgg caagagagct
    19621 ttcaggtgac cttaacggtg acggcgcagt tgacccgtct gactcttcag caatagaaat
    19681 cgttgcatca ggtatggctg acatcgactt cgtaaatcgt acagttattt attaagtttt
    19741 aaaatttcat gtggcacctc taaaaggggt gtcacatgag gaaaaatggt agttacagtt
    19801 catctgtaat tatatatcgg ctgactgcgt agaaatataa tgtcagacga aaaaaatcaa
    19861 cgggagaggt cagctcttta tcccgaaaaa tgaaggagga atttccaagt gaatataacg
    19921 aagaaaattc tttccgtcat attgtctgtt attcttgcag tagctgtttt cggtgtctgt
    19981 gcctctgctg aaggtacggc taatggcgac gaaagcatga cggtaaccgt ttcaaccgat
    20041 aaggatgtat atgctcccgg tgacgaaatt accgttactg tttcgctcaa gactaatttc
    20101 aatatgactg ccttccgttt cccgatattg ttcaattccg atgtttttga aatcccgaac
    20161 cttatcggcc ttacggcact taacaccaca aaggtaaaag gaacgttgtt ggcaaactca
    20221 cttaatgacg gttcagcttc agttgacggc tatgattctt cggaattcag tgttgtcctt
    20281 gtccagtgga cggcaggtat taccgattca accatgggtg tcctgaatct tccgaacggt
    20341 gagcaaagtt tctcattcaa agtaaaagcc aagtcatctg cagcgggtaa agagggtaca
    20401 ttctttatcc cctcggagta taagggattc tacaatcagg caatccagac tccgacagat
    20461 gcaaccacgg tctactatat cgatgatgcg aaatttacaa aggaattcgt ttctaaaact
    20521 gtaagtgttg tcggcgagac agtagatctt gtaccgaata cggcctatga atcacaggca
    20581 gttattgaca aggcacaact gtttgtttac ggtcttgata ccggaattac aacaagtgcc
    20641 gagttaaagc agaaggttat tgtttccggc tccggaacga tgaaggttgt ttttaccgaa
    20701 tacggtctcg gaacgggtgc aaaaatcaat gttgtcgttg ataataatat tgtaaagtca
    20761 tatacaattg ttattttcgg cgatgtaaac ggagacgcaa ttattgatac caatgactca
    20821 tcaactattg aagttgttgc gaacggtatt gaggatttcg gacttgatgc tttagttttt
    20881 gctgccgatc tgaatggcga tggcacagtt gacccgctgg atacagcttc tgcggagtcc
    20941 gctgctaacg gcattacaga tattgatcag gtcaaccctt attaattcct attaaacact
    21001 aatatgggag gaaattttgt atgaaattaa caaaaaaagt acttagtata ttgctcgcaa
    21061 ttgctatggt acttggcacc tttgctgtag ccgcttccgc taacggaaat ccggatactg
    21121 ctccctataa gtcaaagatt tggcttactg catcgccgat cacagccggt gcggagtgga
    21181 caagtaaaac agaatggact gcacctacct ggacagacac caaaacaggt gacatggaag
    21241 ttacagcagg tcagaaaatt atgatcgctg tccacattaa cacaaacttc gacgtaggac
    21301 attgctcaag cttcatttac tatgatgaca gactccttga tccgaacgag attcgtatgg
    21361 ctcagggtta tgctaagtca acacttggta acatgagaac aatgacaagc tacaacgaaa
    21421 gcgaccctta tcttacaaac acaaatttta ataagcgtgc ttcttcatgc tcatcgttgt
    21481 acacaaacaa caacttcttg aacgctctga accgttgcgc agatgatgac ggaaacgttt
    21541 acttcaagaa gcttgtcggt gagacaggca agtatgcaaa cattaccgag gctaaggcag
    21601 caggctgggg cttctatatg tatgatacat acccggatcc cgaaattgca acaacaacaa
    21661 gccttgacgg tactgagtat cttcttgcaa tccccatgca gattcctgag gatgcacagc
    21721 ccggcgacac atacaagttt gtaatgcctg aggacaacat cagacgtgca actaacaagg
    21781 ttagcggtac ttatatcggt cgttgcccgg acggtgacgg tagttcacaa atcgttaaca
    21841 gccttgatta tatctatctt gacggtgagc gttactttga ccttaggggt acagagatca
    21901 ctcttacagt taagggtgcc gaagctcagg ttgattactc aaagcttcag gctaagtatg
    21961 acgaagttaa ggatacagta gttgctaact acaacaacac tgaggacttc gtaaaagcac
    22021 ttgcagcagc taagacaatc cttgacgaga agaaagcaga tcagacaact gttaataacg
    22081 ctcttacagc tctcgaggcc ggctatgcta agcttcagat caagggtgct gattacacag
    22141 ctctcaacgc tgctaaggac gcagcagcta acatcaaggc cgagaattat gagcaggatg
    22201 ctaactggac agcattccag aacgcttatg atgcagcaaa ggctattgca gcaggtcttg
    22261 atattaatca tcagacagaa atcactgcag cagcaacagc tcttacgaac gctattgcta
    22321 acctcactcc gaaggtagtt gaggaagacg ctgattacac agttctcaac gctgagatta
    22381 cagcttctca gaatatcgtt gatacagagc aggcttcctg gtacacagag gctacttggt
    22441 ctgcattcac aacagctctt gcagaagcta aggctgttcc cacaggactc aagaagtcat
    22501 cacagggcac aatcgatgca gcagcttcag ctcttaacgc tgcaagaaca ggccttgaag
    22561 aggcagcagc taactacaca aatattgatg ctcttatcaa agagtgcgac gctctcacag
    22621 caggcgatta cacatcggtt tcttggcaga atctcacaat tgctctcaat gcagctaagg
    22681 cattagcaag agatattaag gccagaaatc aggctacaat cgacacagct tataataacc
    22741 ttaaggcagc taaggatgct ctcgttcctc tcggcaaggc taactatcag ccgcttatcg
    22801 acgagatcaa caagggcaca gcttacacag aggattatta cacaactgag tcctgggcag
    22861 catatcagac agttcttgct gcagcacagg caatggtaga tgcaggtaac ctcaaggaag
    22921 acgagcaggc tcagatttca aaaatggtcg aggaccttat tgcagctaag gcagctctca
    22981 agtttgtaga cgctgactac acagctgttg acgcagctct tgctaagatc ccggcagacg
    23041 ctgatcttgc agcatactac acaacagatt ctgctacagc agttatcgca gcaagaaatg
    23101 ctgttgttcg tggcttgaag aagaacagac agcctgacgt tgataagatg gcagctgaca
    23161 tcgaaacagc agttgcaaat cttaagctta ttccggccga cacaacagct cttaaggcag
    23221 ctattgacga ggctaagcgc gttaactccg ctctttacac agcagattca tacaacgcta
    23281 tgaaggcaga gcttgataag gctgaagctc tctatgctaa gactgacctc actaagaagg
    23341 acaatcaggc agaagtagat gcacagacta aggctctcaa cgatgctatt acagctcttg
    23401 ttccggcagg cgctgattac acaaagctta acaatgctat taaggcattt gaggctctta
    23461 cagagtccga ttggacagca gatacatggg tagttgcaaa ggctaagtat gatgcagctg
    23521 tagaggcttc caagaaggtt tacacagttg ataagcaggc tgagcttgac gctattgccg
    23581 atgcacttac agaagctatc agcaagctcg ttccggcacc ggctgatttc acagctcttg
    23641 acgctattct taaggatatc aacatatatc tcacttcttg ggcaaaatat gttacagatg
    23701 attacaagac aagggctaat gcagccgttg agtctgcaaa tgcagccgat ttccgtgcac
    23761 ttactaagaa ggatcaggct actgtagacg caaagacagc agaaatcaca gaactctata
    23821 acaatcctga gttccttgct tgggattaca caaagatcaa cgaagctaag gcagctttcg
    23881 cagcaatcga ccgttcgaag tatactgacg attctcttgc agcagtagaa gctctctttg
    23941 cacaggttgg cgacgattgg aaacaggatc cgagaaagcc ggaaggcgca cagcagagtg
    24001 catatgcttc tcagatgctc atccagtcca gagttctcaa gtgggaaact ctccttgttg
    24061 agaagccggt tgaggagaag gctgattata cagctctcga cgcagctatc aagacagccg
    24121 aggaactcat tgctaaggga acatccatct acacagatga ttccgtaaag gttctccagg
    24181 atgctcttgc agaaggcaag gctctttcaa gagatctcct tgcttccgag cagggaacag
    24241 ttgacgcagc aacagctaag atcaacgctg caatgccgct cacagagaag gacgcttcct
    24301 acacagctct cgacgcagct atcgctcttg ctaagacaaa gaatgccgat gattatacag
    24361 aggcttcatt caaggcaatg acagataagc ttgcagcagc cgaggcagtt gcacgtgacc
    24421 tcaagattac agctcaggca acaatcgaca aggctactaa cgagctcaac gcagctatct
    24481 ctgctctcgt tcttaagccg acagaggtta agggcgctat ccagttagtt gagtggactc
    24541 cgtctactga ttccttcaac acattcagtg taaaggttaa ccacgttgac ggcgactatg
    24601 cttataagat ccagtttatt gatgctgacg gtaacacaag aacaattaca agacgcaaca
    24661 aggccggtat cgtaacagca gctactgtta agacctatga tgcacagggt aacgagtgct
    24721 cagagctcag tgcagatgca gcatatgata tttggacaat caacacaaga attgcaacag
    24781 gctgcgagat caaggttatc gctaagttcg gttacacttg ggagagccag gatgttgctt
    24841 acaagttcac agttgatctt gttaagccga ctcttgacac agcagtatat gccatcactc
    24901 cggctgctac agcaggtaag gttggccgtg tcggtgtcaa ggttgagaca ggtatggata
    24961 tccagggcgt tcgtacagta atgtcaacag gtgcaaccct cactcagaag gcttacacag
    25021 ttgaaaacgg ccataaggta ttcgatgctc aggcaagctg ctaccttgag ggcgaaaacg
    25081 taatcaaggt acaggttaag tatggcaacg agtggcatga cgctgattcg ttcacataca
    25141 cagcaacaaa ataagttgaa cactgataaa tcggtgtgaa actccggagg gggtgcaggg
    25201 taacctgcac ccccgtttct atgagcattt ttcaatataa taaaatagaa attgtttctt
    25261 ttttaacaaa gtctttttgc aacttttgta aaaacctaca aaaaaagtaa gaattacccg
    25321 ttataatgtg cattttgtat gttgaaaatt gctcaaaaaa aggatataat atagtcgtaa
    25381 aaaattgatt tttacgaaag gaatgaattt ctatggaatt aacgactaac aagtccgtac
    25441 ttgactggat caacggaaaa gttgaccttg ttaagcccga caaaatcgta tggatcgacg
    25501 gctctgagga gcagcttgac ggccttcgca aagaggctgt tgagaccggt gaaatgatta
    25561 agcttaatga agaaaagctt cccggatgtt atcttcacag aacaaagccg aacgatgtag
    25621 cacgtgttga ggacagaact tttatctgct caaagaagca ggagaacgcc ggcccgacca
    25681 acaactggtg tgatcccgac gagatgtata agagacttta cgatatagca agagattctt
    25741 ataagggcag aacaatgtat gttatcccgt tctcaatggg tcctatcggc tcacctcttg
    25801 caaagattgg tattgagctt acagactcga tttatgttgt tctcaacatg gctattatga
    25861 cccgtgtagg caagaaggtt cttgacgtac tcggagacag caacgattgg gttcgcggct
    25921 tccatgcaaa atgtgatgtt gatcccgaaa agagatatat ctgcctcgac acggtctatc
    25981 tctccttccc
//
LOCUS       DAWBSW010000006        23695 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_458_length_23695_cov_2.713409, whole genome shotgun sequence.
ACCESSION   DAWBSW010000006 DAWBSW010000000
VERSION     DAWBSW010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 23695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23695
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_458_length_23695_cov_2.713409"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    856..865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9828..9837
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16141..16245
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17178..17187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17707..17716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21440..21449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6830 a         5241 c         5136 g         6333 t
ORIGIN      
        1 cccgaaaaga gaacaatgtc gttgctgtta agatacatta ttttttcaac ctcgtttcaa
       61 agcagagcca gccgcagctt tcgtgcctgc cgacgacctc aaagccgtac ttttcaaagg
      121 catcgagaac gtcctgctcc cttgtgtcaa taattccgga tgttatataa attccgtcat
      181 ccttgagaaa tctgccgaca tcctttgtaa acatcacaat gatatcggca acaatgtttg
      241 caacaacaac gtcgtatttg ccgctgacct tatcggtcag gttaccctga aataccgtat
      301 atttcggctc cgtaaagccg ttcatttcgc cgttttccct tgccgtctta accgcaagct
      361 cgtcgatatc aacccccgtt gcactccttg cgccgagcaa cagaccggca atgccgagga
      421 ttccgcttcc gcatccgata tcaagcagct ccgtatcggg cgttatgtga ttttcaagcg
      481 tttcaagaca aagccgtgtt gtgtcgtgtc ctccgctgcc gaaagcaagt ccgggttcga
      541 tatcaagcac cgttctgccc tgcggatcat agtcgttatc ccaaaccgga cgaatcagca
      601 gcctttgacc gacaggcatc ggcttgaaat actctttcca gttattttcc caatcctgat
      661 tgcggcacac gctgaggtcg atctcatgct taataccctc cgcattatag cgttcggaaa
      721 gaaaagcaac cgcctcggca ggactctcct cgggagaaat ataaatatga attgtcgcct
      781 tgttcctgtc ctttttaagc agatcctcat caatcaagtc gatatgggca atttcccatg
      841 ccgcctgttc aaggtnnnnn nnnnnctgta atcctcaata taaattccgt acggaacaac
      901 catttgagcg atgttgcctg ccgtttccac gtcctttgaa tcaatgttaa tttttacttc
      961 tgtccagtcc ataaaatctc cttatttgat ccagccgatg ccgttcttaa taagctcagg
     1021 gatattgatt atcgccttaa aaagcacctt cgcaattgtt ttaactgccg tcataatgta
     1081 atcgctgagg ttaaaggttt cggtattctt gctttcaatc ggctttacct catccggatt
     1141 tacggtgtag gcaagctttg ttctgaccgc cgcatggtcg gaaagatgtc cgttccatga
     1201 aatcccgtcg cagtcgaagc cgtcataaac cgcttcaagc aattcaaaag aaacacctgc
     1261 accgtcacga taataaacat ggtcgagtgt atcccatgtt ctctgatagt cacatccgta
     1321 acgctcccac atctcggaat aggtaatgtc atagtttccg tcaacattat attcgatcca
     1381 agcatcctta aagcccttgc cgacaaagtt gtcaatgagc ttttgacaaa gatcgatttt
     1441 atatgttccg ttctgatatc cggcacggaa aacagtgtag tttgtattaa aatcacccgt
     1501 caaaataaca gccctgtccg cacccgaatg ttcctcaaca agctcgataa gctggtcaaa
     1561 ttgatcggcc tttgcgagca tggacttttc atcctcccat gcgtcggcat gaatattgta
     1621 aacatcaata aagactccgt cggctatctc aaccgtacaa tagagtatac ccttcggcgt
     1681 cagctcgtcc gagccgcagt cgtacacgcc gtatgccgtt ctccacggaa ctctgccaac
     1741 gttataaaca ggatattttg agaatacatt cagcccgtct ccgacggcaa ctccgccgct
     1801 cgtcagcgtt gcatatttca tgtcaagatt cttctcaaga atcgggtgga ggttaaaatc
     1861 ctcctgtacg ctgagaatat cgcagtcgga tgcattgatt tgctttgcaa taagcccggt
     1921 cgccttgatc gggtcacgct tggttgaaga aagcgccgcg ggaatgggaa gcccgtcaac
     1981 attgaacgaa acaaggttca gcgttccctt tatcgtgttg tcattctctg caaaggcgag
     2041 cgacgtgcag ccgacaaaaa gaacaactgc caataaaata ctgattattt ttttcatgct
     2101 tactcctccg ttcaaagcaa gtccgctttc ggcttgattt ctttaagtcc gtgattgtaa
     2161 agctttctgt tttcaagcgc ccactgtctc ggcgtgaaat cgccagactc aacctcggaa
     2221 accgcctgcg aaatctcata tattcttgcg tcaagcaagt caagctgcga taggatttcc
     2281 gcttcaagaa acatcggtct gaccgccgct ccgaactcag gctcaccgtg gtgagaaatc
     2341 accatatgct caatgagcgt caacagctct ctgtccgtgc cgagctcctc tccgattttt
     2401 tcaatattca ttgcgccctt aacaaggtgg ccgataagtg ttccctctac cgtgtagccg
     2461 gatgcaattc ccgtttccga gacctcaaat tcaccgattt ttgcaacgtc atgcaggata
     2521 acgcccgaga aaagcaagtc cttttctacc gagggataaa tctcggctat actctgcgcc
     2581 attctgagaa ttgaaagtgt atgataaagc agtccgcctc tgatcgcgtg atgaagcttg
     2641 aatgccgccg gcaaatagag cattgccgtc ctgttctgct caagcaccgc cataacaaga
     2701 gttttgagct gtgcgttttc aaaatcattg acaacgtcga ctatcgccga atacatcgac
     2761 tctccgttat attccgcact tggaacataa tcctcaatgc gaacgccgtc gttttcattc
     2821 gcaaggcgaa ctctgtcaag tcgcatctgc ggcgcatcat tgaacattga tatagatccg
     2881 cgaaccttca gcagcgaatt ttgttttata aatccgaaaa cctcttcctt atagtcccag
     2941 agctttgctc cgatttcacc ggagttgtcc gtcagaataa ggtcaagata aggaattcct
     3001 ttagcggttt ttttaacctc aagatttttt acaaggcaaa agccttctac cgtgccgttg
     3061 tcgtttattt caatgaaatt catatgaacc tcccctatga gataaatata tattgattgt
     3121 atcacatata aagaaaaaaa gaaagaattt ccttgcaaaa gatgtcgctg tatgttaaaa
     3181 tttttttatt aaggtgattg tcgttgaaat ttctcaatgt tttaccgaga gagggtgctt
     3241 taatgagcaa aaactatcat ttttttgcaa tgctttcaag aatgaaatat atcaaccgct
     3301 gggcgctgat gaggaacaca tatatggaga acatcagcga gcattcgctt gaggtcgcaa
     3361 ccgtggctca ctcgctcgcc gttattcaca acaagcgttt cggcggcaat gtcaattccg
     3421 agcgtgtggc agttctcgga gtattccatg atgctccaga gattatcacc ggtgatatgc
     3481 cgacccccgt caagtattac agcaagcaga ttcgtgacgc atacaactgc gttgaggacg
     3541 atgcgtgcaa ggcacttttg gagatgattc cggaggatat ccgcgaggaa tacaaaccgt
     3601 tctttatcaa gcaggaagcc gaccgtcagc tctggaaatt tgtcaaggcg gcggacaaaa
     3661 tctgtgccta tacaaaatgc attgaagaat caaaggcggg taatcacgac tttgacaaag
     3721 cggcgaaaac aaatctgaag gggcttgagg ctctggatat gcccgaggtc aagtgcttca
     3781 tggaggattt tctcccgagc tacagcttga cgctcgacga aatgaactaa agtcattatt
     3841 atttgagaga ggtatcaata tgggtaagta cacaaaagaa gacattctca gaatggcgag
     3901 agagcaaaac gtcagattcg tgcgacttca attcacggat attctgggct gcatgaaaaa
     3961 tgttgcaatc acagtcaatc agcttgaaaa ggctcttgac aacgagtgca tgaccgacgg
     4021 ttcttccatt gagggctttg ttcgtattga agagtcggat atgtatctct atcccgatta
     4081 cgactcattc gttgttcttc cgtggaatca tctttccgac ggcaaaacag cacgtcttat
     4141 ttgcgacgtt tactgctcgg acagaactcc attcatcggc gacccgagaa acgtgctcaa
     4201 acgcgcaatc aaaaaagctg atgacatggg atataaattc aacgtcggtc cggagcttga
     4261 attcttcctt ttccatacgg acgacgaggg cagaccgaca accaaaactc acgacaaagc
     4321 aggctacttt gaccttgata acgttgacct cggcggcgac tgccgacgtg atatgtgtgt
     4381 agctcttgag gatatgggct ttgaaatcga agcctcccac catgagtgtg ccgagggtca
     4441 gcacgagatt gatttcaaat acggcgatgt tctcagcatt gcagataaag taatgacatt
     4501 taaatacgtt gtaaaaaaat atgcccagct tcataatctc tatgcaacct ttatgccgaa
     4561 gccgattttc ggcattgcag gctcgggtat gcacacaaat atgtccctct ttgataaaga
     4621 cggcaacaat gccttctgtg accctaacgg acagtacgga ctttcggaga cagctctcca
     4681 ctttgtcgca ggtatcatga agcatattaa agcaacaaca gccatcaata atccgcttgt
     4741 caattcatat aagagactgg ttccgggcta cgaagctccc gtctatatcg catggtcggc
     4801 gtcaaaccgt tccgccctcg tccgcgttcc cgccgcaaga ggcaagggaa caagagtcga
     4861 gctgagaagt gcagaccctt catgcaaccc atatcttgaa atggctcttt gcctgcttgc
     4921 aggtcttgac ggaattgaaa agggctatat gccgcccgaa tcgaccgtta aaaacatatt
     4981 tgacatgagc attgaggagc gcaaagcggc tcaaattgag tctctccccg gcactcttga
     5041 ggaggctatt gacgagtttg aaaagagcga cttcatcaaa gaatccctcg gcgagcatgt
     5101 ttatacaaaa ttcatcgaag caaagcgccg cgaatgggac agctaccgca ccagtatctc
     5161 tcagtgggaa atagacacct atcttgcaaa atactgattt caattcttta taaagtcaac
     5221 cccgtacctt gcattacgca aagtgcgggg tgttttttag ctttcttcgg cgctgtcggg
     5281 ttttgaaatg aagaatcccg agcctttttc ttttagcagg atcaatgaga cctccagcaa
     5341 aagcttaaaa tgcaaatcgg aatcatcaag cggaatttca aaatcctctt tcatctttct
     5401 tatgcgatag aggatcgtgt ttcgatgagt aaagagggct tcgcatgtat ctttaagtga
     5461 atgatcgcat gctaaataat agtataatgt ctcgcagtat tgactttcct tccttctgtc
     5521 ataatccgcc agcgaaagaa tcttttgcga aataagcttg tcatagtctc gcattttatt
     5581 cagcatgatc ggaaccgcca attgggaaac ataaaagaca ttgccttgcc cgacgctctc
     5641 atctacgact atattcagtg cttcaagcac caaatcatac attttcggaa tgttataaag
     5701 ctcatctacc gggtttgata ttacaacctt aagatgaaat tcctccgcca acttttcaaa
     5761 agcttttcga tcatatccct tttgcaggaa cataaacacc tttcctttat gtaagaaagg
     5821 gtgagattca taaaacctgt atgtcagctc gtcctttaac ggccttttcc ctatatattg
     5881 cgaacggtaa gcaaccaagt caatcaacgc aaaagcaacg ggatgaaaat cggcaagata
     5941 ggttgatgat atttgcagct taaaatatga ttgcgattga aagcctccat ccaaaaggtg
     6001 tatcataatt tcttccgctg tttcaaacgg tctgtcgacc ctgcctgcct caataaacat
     6061 ttgcttagcc agaatattct ctattcgtat aaatgtttcg ggcgcaacgc tgccgagttt
     6121 attgtcaaca tcaatgcaaa tcaaatagcc gagtcgaatt cctccgctga taagaggagc
     6181 aaatctcctg ctgtatgagc tgtcctccag ctctacaaaa tcagccattc cgccgacaag
     6241 ctcatcgctt tcgcttatca gcgcaccggc ttcatatgta atttcgccaa gcttaactgc
     6301 cgaatcgaaa acatcgtcgc tgaatccttc gggtgagtaa tgcgccgcca cacggtaagt
     6361 gtcatcaatc accatcagcg gacagcctaa gattttaccg gcatcttcag tgagcaaatc
     6421 aatatcgtcc cgatcaaaaa aatccgatgt caatattgat aatttatcat caacagacat
     6481 gagctttcac ctccgaatgt cattgtgctc acagcacaat atatttcaaa attattatac
     6541 tcccatatta ttgaaattgc aataaaaatg agtaaaatat aggtgtaaaa agaaaagaga
     6601 gaaaagtgaa gaagagaaag gaaagaaaaa taagagaaca aaaaatgtat atcctgtttg
     6661 gcggcattga gccgtcggac ttcatataaa aaacaattat aacggaggtt ttatttatgt
     6721 taccgagtat ctttagtgaa aacatgtttg atgatttctt taacgatccg ttcgatcgtg
     6781 aattcttagg tgcacataat ccgttatacg gtaagcacgc aaagaatatg atgaagaccg
     6841 atatccatga aaaggatgac ggctatgaat tggctgttga gttgccggga tttaagaaag
     6901 acgaaatcaa cgtcgatgta agtgacggct atcttacaat caaagcagcg aagggccttg
     6961 acaaagacga aaacgacaag aagggtcatt tgattcgcag agagcgttat gccggagctt
     7021 gctcacgcag tttctatatc ggcgatgtaa agcctgaaga tgtaaaggca aaatatgaat
     7081 cgggcgttct cactcttgaa gttccgaaag cggaaaccaa aaagctttct tcaagctcat
     7141 caatagcgat cgaataatca atattcggcg cagaccgatt tcaaacatta aatcggatgc
     7201 tgagacagca attgtctcag catccgattt ttttttgctt taaaagtttt gatatataaa
     7261 attctgaagc gtaaaaggaa caattgcagt aattgaaatt atatcaatca ctgcaatcat
     7321 tctggtggga gagggtggat tcgaaccacc gaagtcactg acgacagatt tacagtctgt
     7381 tccctttggc cactcgggaa ctctcccata ttcactttta aaaagtggag ctggtggacg
     7441 gactcgaacc cccgacctgc tgattacaaa tcagctgctc taccaactga gctacaccag
     7501 cgtctcaacg ctcgtatact ataccacaac aagaaccgtt tgtcaacact ttttttaatt
     7561 ttttattttt atcttgaact gaaactttaa aagtgatata gttatcgtat tgttttttcg
     7621 aaggggttat attatgaaaa aaattattgc tgtctttatg gctatatcac tcagtttatc
     7681 ttttgtttgt atacctgttt gcgcggctga cgacaacggt atgatttcag ccgttgtaac
     7741 cgatgagcga gctcagagac aagccgtccg tgccgctgct gctgccataa tcggcgaaag
     7801 tccgagtgac agcataaccg taagcaacaa ctttgtaaat gatattcttg acaatgtcgg
     7861 tgaaaatccg acaccggaga atatttccga cgaagtatca aaatacgcag gcattttcaa
     7921 aaataaaaca gatgaatacg ctaaggtcat tgcagacgat gccgaatatt acattgccga
     7981 tgacggcgaa ttctttatta tcatcgatat cacaaagcat cccgaggtgc ttgagcttac
     8041 gataatgtgc aagacggcaa ggcttatgct caataagcag aatgagattg catctcagag
     8101 cggcagagag tgcataacaa tggattaccg ccatgttgtc ggtgagcttg cgctgcacaa
     8161 cacgatttat gcactgacca aagccctcgg aggcgagaag gcgaacagtc ctctgcatgc
     8221 tcactacgaa agcgcaaaga ttgccgagct aaataaaaac gagcaaagag gagcggacta
     8281 tattcgcgtc gtcggaggat ttcttgacat ttgctacagg gttttctcaa cgatttttca
     8341 tcagttctta taaatctcat aagccataaa aaagcggaag ccgaacgact tccgctcttt
     8401 atttttatgt cttatttaac actttcaaca agatccatga attcggaaat aacctttgaa
     8461 gccgtgttct taccccaaga gataaggcac catgtatccg tatcaaaaga ttctttcttc
     8521 tcgctgtact gtacacctgc cattacgaaa tcgtcatccg gctcaatgta tccgatagca
     8581 tcgttcataa gatcgaatac gataatgttc tcgccgtaca tttcgcgaag cgacttatat
     8641 ttgaagccct tcagaccatc gccgcccata aggagttctc cgtatgtctc gccagggcaa
     8701 agctcaacga gaagtgaatc gccaagctcc atgtagcccg actctgtgac tgtatagtac
     8761 ttatcggtcg tcttgtcata gaggaagaaa taatccgtga tacctgtctt gccgacgata
     8821 tccataagaa cgttggaaac ctcaatgata tactgcttgt gagcaatgtt gagcatcggg
     8881 gcaacagtcg tctcgtttac ggacttccaa tccttgtacc atgaagtata tccctcattc
     8941 gaaccgtact cagcctcgcc gaggaaatca ccgagagtat agttcattgt cctgcattcg
     9001 gcttcactct ttgtaagtcc gagaatgata tagcctatct catagccgta tctgacagtt
     9061 tcctcatgtc ttgtaaggtc aagtccgtca tcagatgcac cgcgctgata agtgattgtt
     9121 ccgatacagc cctgaatata aatgaagtta tagcctgcct tattgactac ctcctcaata
     9181 taaggaacga aatcggcgga aataacaaca tgaccgtatc ccgaattctc ggggtgagcg
     9241 ctgaacgaag caataaccgt tcccttttcg ctcttgttat ccggagtgaa catcaaccgg
     9301 taaagattgc cgtcaaatgt ataaggagct gttctgtcac gaagataatt gtctgcaata
     9361 tccttctttg caagcgtaag tgtgccgccc ttcatgtcgg aataagccga cttgattgta
     9421 gccgtgcact ggtcaacgat cttcttgagg aaggtgcggt taacgccgct gtgaatctcg
     9481 gaaagtccga gatttgaaag gaaaagattg ttcagaacat ttggaacggt gttatcccat
     9541 acgccctgga ggtctatgcc gctgtgggta tgagtaacgg aaacatttac gctgacaata
     9601 ttgttctttg ccgcaaaatc cgagatagct tcacggataa gtcgaacgtc cgcatttgca
     9661 attccgattg cgtcaagcac gcagaaagct acatttcctc tgcctgtgcc gtcattgaga
     9721 acgatcgttc gaacccagag gccgtcaaag gtctcggttg ttgttgccat aaggccgtag
     9781 cctcccatgg tgtacggtgt tgtgccgaaa tcatcgggaa gaatcgannn nnnnnnngcc
     9841 tgggcataac cgagatgcca ttttgcacct gccgccggag cgtcaaggaa atccgacatt
     9901 cccttgtaga aattaccgta gctgtcaaga tcaaaatcgg cataatcatg gataacggaa
     9961 gttttcggaa taacaaagcc gagagccttg ccgataacat tgatgagcgt attgctcaaa
    10021 agattgataa ccttggcaag cagcttcatg aaaaccgtct tgggactcgt tgaagcaagt
    10081 gagccaaagg catcgccgat tttgtcaagt gtgtcgttcg cgttgctgtt aactacctga
    10141 acacggttgc cttttgcagc cgcatcggca aggtactctt caagagtcac gcttgactca
    10201 gcaacaatcg gagcttcctc gttctttgca aatgccgtta cgccgatcgg aacgatcaga
    10261 caaagacaaa gagcgaccga gagaaccgac aaaagaattt ttttcataat tctgtctcct
    10321 ttcggaaaat gatattattc ctatgatact tgattatatc acaaaagtcc gtttttttca
    10381 aataaaacaa tcgccctctt ttttattcaa attgacgaaa gtataatgcc tatttcaatt
    10441 gacttttgtg cctgacaaga atataatata atttgatttt attattgtac acagtcaatc
    10501 ggaggttttt gtatatggat ctatcaggca aaaaggttct cgttgtcgga cttgcacgca
    10561 gcggcatggc ggcaatcaga gttcttaaaa agctcggtgc agaggttact ctctccgaaa
    10621 gcaagaaaaa agacgatata aaagaaattg gttttcttaa cgaaaacaat gttgagatcg
    10681 tcggacagga tatggcggtt tttgaacgtg attttgactt tgtcgtcaaa aatccgggcg
    10741 taccctacag aagtccgatg atgcagaagc ttcaagagcg caacattccg gttatcacgg
    10801 aaattgagct tgcgtatcag gttgcgaagc ctcagcatta tattgcaata accggcacaa
    10861 acggcaaaac cacgacctcc actcttacat atgaaattct tcgccgtgct tttccgggca
    10921 aggcgcatct ttgcggtaat atcggtattc cgctttgtga aattgttatg gaaaacggcc
    10981 ttatggaaga gggcggacac tatatcgctc tcgaaatttc aaacttccag cttgttaata
    11041 ttgataagtt ccgtcccgag gttgccacga taattaacct cactcccgac cacattgatt
    11101 tcatgggcag ccttgataat tattacaagt ccaagatcga ggtttacagg aacatggcag
    11161 gcaatgacgt tttcctgctc aacgcagacg atcccgtcgt taaggaatac accgaccgat
    11221 atcccgtgaa gtgcgctaaa gaaagcttca gtacagacag ccaaagcgcc gactgcattg
    11281 ccaaggacgg ctatttagag ataaagggcg aaaaaataat ccctcttaat gcaattaaaa
    11341 ttgtcggcaa gcacaatttg cagaatgtca tgattgccgt ttcagccgct aaggctatag
    11401 gcatttcaaa tgacgttatt cttgaggctg tttcgtcatt taagggcgtt gagcatcgaa
    11461 ttgaatttgt acgtgaaatc gacggagtta aatactataa cgactcaaaa ggaactaaca
    11521 ccgacgcaac aataaccgct ctcaaggcct ttgaccgcgg tgtgattctc cttgtcggcg
    11581 gatttgaaaa gggcttgccg atggacgaga tgaaaaagca cctcggctgt gtcaaaaaga
    11641 ttatcggatt cggcgcctgc ggtgcaagac ttgtgcatga ccttgtcggc gaggacggaa
    11701 ttgtcgttac aacacttgat gaagccgttg cagaggcaaa caaaattgcc gaaagcggag
    11761 acaccgttct cctttccccc accaccagct cattcgacca gtatacctgc tttgaggagc
    11821 gcggcgacca ttttaagaag attataaatg ctctataaac cgaaaggaaa aatatgacag
    11881 aaaaagagaa atacatcggc gttttcgact cgggaatcgg cggactgacc gtcgttaaaa
    11941 gcatagtcga aacaatgccg aacgaaaaca taatttactt cggcgacacg gctcacgttc
    12001 cctacgggac acgctcaaag gaacagataa ccgaatacgt tctcagcgat gtaaaattcc
    12061 tcagcagatt taatatcaaa gcaatagtta tcgcctgcaa cacggcagat tcagcagcaa
    12121 gagatacggt tgaaaaaaac tacagcgata tccctgttta cggcgttgtt gctcctgcgg
    12181 ctaagaaagc ggcaaagctg acaaagaata aaaaaatcgg tgtaattgcc accaacgcaa
    12241 cggtcaacag cggcgcatac gaaaagacga tacacagctt tgattccgac gcacaggtta
    12301 taagccttgc gtgtccgctt ctcgttccgc tcgttgaaga aggacgtttc aaaaagggag
    12361 atcaggtcat cgaggaggtt ctgcgtgaat acctcactcc gctgaaagag cagggcattg
    12421 acacactcgt tctcggctgt actcactatc ccctgcttta tgacattata accgagatta
    12481 tgggcaacgg agttacagtc atcagctcat cggttgcggc ggcagaggaa ctgaaaaaag
    12541 gtctcggaga aagaaatcta ctcagcaact caaaaataag cgaacgaaag tatttcgtca
    12601 gcgacaatgc agaggatttt gaaaagcacg ccctgatatt tatgggcaat gccctcggcg
    12661 gcacagtcga acaggttgat atcgaagaat aaaagaatac cgcataaaaa atcgcagatt
    12721 aacggaaact actccgaaaa tctgcgattt tattttttgt ttgtcaagcc caaaatgtaa
    12781 tcggtcgagg tgttataaag cttcgccaat gcaatcagca catcggtcgg caggtcacgg
    12841 taaccgtttt cgtatcggct gtattgaggc tgtttcattc cgagataatc cgaaacaaat
    12901 ttttgcgtat aatccgaatc ctctctcaaa tcttttactc tgcgataata tgacaaaaaa
    12961 tttcgctcct tataaccaat tagttattga catattaaca ttatcatgtt ataattgctc
    13021 caatatataa ccgtttggtt atatagtaga aaggaagtgt ataaaatggc aagattagaa
    13081 agtaccagcc tgtcggtatc accgagcaag gaacaagaag caattgaaac atatcaaaaa
    13141 ttcggttggg aattgaaatc atcgcaagag attttcaaca aagattctca caatgaagtc
    13201 agaggtgatt ctttatatag cgtcacagaa acgaccaatt atgttaaact tgtttttcaa
    13261 cgtgataaag atatgccgta ttataatgaa atatgcgaga ttgaaaagaa gttttttgag
    13321 gcattaaatc gagaaccgtc gtattcatat agcaaggctc cgttaataat tggaatcatc
    13381 atcgctgtaa tcggtgcgtt tgttttattg tcaggtgacc ttactgaaaa attgatcgga
    13441 ttgcccatta ccgcattagg aatattaatt attgtttggc gagtaaaaac caagaacaaa
    13501 aaagaagcag aatatgatga gaattataaa aaatggaaca acgaatgtac aatgcttctt
    13561 gcagaggttg agaattatat ctaatggaca ctcaaactgt aaaatgtcca aattgcggcg
    13621 cagcaataac cgaaaagccc aagtcagatt ttgtaaaatg tgcattttgt gatgctgttt
    13681 ttaaaatgtc aaccaacctt atcagcccaa ccacatcgga ttctgtgaaa tatggttttg
    13741 ttataaaaag taatgttttg acagaatata acggagaaag cacagaaatt attgttccct
    13801 ccaatgttga gaaaatatcc gatggtgttt tcagaaattc aggtttaaca agtgtattgt
    13861 tgcctgaatc tttaaaagaa ataggggcat atgctttcgc tgactgtaga aatctttgta
    13921 gcattgttat cccaaaaaat gtttactata ttggcaacag agctttctgg cgttgtacta
    13981 acctttcgca aattgatttt ctcggagaga atacacaatt aggtgaaggg gtaattttag
    14041 gtacagaaat atacaaaaag tttttagagg aatacgatag cgaagtcaag gctcaattag
    14101 agaaagaagc ctttaaaaga agaaaagcat atggtctgtg cccatattgc ggcagtaatt
    14161 acaatatctg gggcaagtgt aaaggttgcg gcagaaaaaa agaatagcca aatttacaaa
    14221 aagactgcaa atgaaacaca atcatttgca gtctttgtaa tattttattt aaccaactca
    14281 ccgacataat cggcatagat ttttccgata acctctgcgc cgcggtcata ggtcgggtga
    14341 acgccgtcgg cggaaatctt ctgccaattc tcgtggcaga tatgcgatgc aagcatttgt
    14401 acatgacacg gaagcagaag attcccgaaa agtcaattga cactttggca attgtgctgt
    14461 aatcatgctt caattatggt atgattaaca taataaaatg attatttcag tcacagaaag
    14521 gagcaaacat atgcaaaaaa caaacataaa aaccaaactg ttgacagttc tgctcacgtt
    14581 gtgcatggta ctgtcaccaa tgtcagtttc ggtgtttgcc gtaaatttgt cagttaagaa
    14641 tgactctgag ctggaggcag catttgaaaa tgcagactgt acggagatca aattgggtga
    14701 aaacattgag accgtttggg aacttgatgt agaccgcacc gtaactcttg atctgaacgg
    14761 atatacactg agttgcagtt cgacggacga agatataatt cgggtacgca gcaacggaaa
    14821 ccttacggtg aaggacagcg gcatcggcgg caaaatcgac ggacagaaca agaactgcgg
    14881 aattgaagtt aagggcggca cgctgacctt agaaagcggc agtattgtaa actgcacaga
    14941 tgccgacggt gacggcggcg cagtggatgt ggcaaacacg ggtgtaacgg aaacaccgat
    15001 caagtacggt aagttcatta tgaacggcgg tgccatcatg ggctgcaagg ccggcgatga
    15061 cggcggtgca gtggatatag gtagcggttg caccttcgtt ataaacggcg gaaccatcag
    15121 taattgcaga gctgatgatg acggcggagc cgtatttatt aagcaaggcg gagcctttga
    15181 gttaaatgac ggtatcattc aaaattgctc tgcaggtgcc aacggcggag cagtgaatat
    15241 ttatcaagac ggtcacttta gcatgacagg cggcacaata aaaagctgca aagtggatct
    15301 tggcggactc ggcatggcgg tgtatggcag taacgataat gctgtagtca ccatgatcgg
    15361 aggcaccttt gaagattgcg gagcattccc ttattccttt gatgaattca ctgtgacatt
    15421 cgattctgac ggcggaagca cagtgcccga gcagaagctg cgaaacactt cggcagtcaa
    15481 gcctgcagac ccgaagaagt caggctataa ttttgtcgga tggtatctgg gagaggaaga
    15541 atacaccttt actgccaatg ttgtcaaaaa cattaccctg aaagcacatt ggacacccgt
    15601 agctacgccg atcaccatcg ccactgccgc tattggaaat gcaaaattca actatcagcc
    15661 cggcgatgtt ccgcaggcaa cggcatgggt ttcagaggtc gatgcagata aatacgagat
    15721 cgcctatgaa tgttggcagc agtttgaaaa caacgaaccc gttgccgctt ggtattccga
    15781 taacggttca cacggttctt tgccgaccat cgcaaaattt gaaagcggta aaaaatatgt
    15841 atattccatt atgctgaagc cgaaagacgg atattccttc agcagcgaaa ccgtcattac
    15901 agtgaacgga gaaaaagtat cggcgccttt tgtcggcggt tcaatgtata tccctgccgt
    15961 taaaacgatc actatgcaaa ctttgattgc cattgatgta gtagagatca acgatgtcac
    16021 cgtcagcttt aaggacggcg ataaacctgt gtttaccggc aaggttcctg acggtgcaaa
    16081 ctacgcatac cgatgtgagt ggtgggagct ggacagcagg accggcgcaa tgtctacaga
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtcgg aaacgtccgc
    16261 tatatattca cacccgatac aaagcttaaa atcaacggtg aatttgtgaa ttatacacgt
    16321 tacgaaggcg atgagagcga cggttctgac ggcaccatgt gggttctcac cgatctgaca
    16381 atgacacccg aagaatctac acctcaaaaa catagctttt tagattggtt tatcaatctt
    16441 ttcacaaaag tcgttaaatg ggtcattgac tttatcggaa aggtttgcta aatgattaaa
    16501 aacagcgtcc tcttgttcaa tagaaaaagg ggacgcaaat tttatataat tttgcttttc
    16561 ttataataac aaagatatat gttattcaag aatcgagact cttatttaac caactcaccg
    16621 acataatcgg catagatttt tccgataacc tctgcgccgc ggtcataggt cgggtgaacg
    16681 ccgtcggcgg aaatcttctg ccaattctcg tggcagatat gagatgcaag cagtccgtca
    16741 agcggaataa aaatatcggc atattcacgg gcgagcttcc ttgtaatctg aatcttcggg
    16801 tcaaggtcct cacggaaata gagcttatcc tcggcaggaa gcaggaactg ctccatcata
    16861 acgatttttg catttgtctt tgtcttgatc gcttcaagaa ccgtgcgata tcttttttca
    16921 aactcggcgt tgtcgagcca ctctctcgta tcggctctgt gccatgtgtc gttgacaccg
    16981 atgagaatcg aaacaatatc cggctgaatg tcgataaaat ccgactgcaa cctgttcaca
    17041 agatcaatcg tctgattgcc gctgattccg aggtcaataa attcaaaatc aagctccggg
    17101 aatttttctt tcaaaaatct tgccgcatcc ttcgggtatc cgttgccgag gttatgcgga
    17161 tcttcacggt cacggtcnnn nnnnnnncgg taatgctgtc accctgaaac aaaatcttca
    17221 tataaagtcc tccgttttaa cattttttat tttatcaaaa tatctgtctc cgcaaacgga
    17281 aatcaaaaca tttttttcac tcaaacatga aaactttttc tcatattttt caaaaaaggt
    17341 ttcgtaatct ccgaaagaaa attctccgag atctgcaagc gatttcccct cgcattttat
    17401 tgcgctttct ttaagcgtcc atattctcgt aaacgctttt gctttgtcgg gcgaagcgtt
    17461 aatttttttg ttttcgctct cattgaaaaa tctttttgca accttctcgt taaagtcgcc
    17521 gatttcctgc acgtcaattc cgacaggcgc aatatcaatc gcaacagcca ctccctcggc
    17581 acaatggctg atgttaaaaa acaattcacc gcttttcaaa aacggctttc cattctctcc
    17641 gataacgatt tcaaaatttt caattttgtt ttccttcaac gcaaagcgaa gcaaaagata
    17701 agcaacnnnn nnnnnnaccg cgcaattgtc acggtcattt tttcttttta gtctgttgaa
    17761 tctttcctgt cggttcttcg ggagaaattt taaaaaattt tcggcagaaa aattatcata
    17821 atcattaaaa aaataaatca ttttcaatta aaaacgtccg gctttaacgc cggtggcatt
    17881 atgctcggca atcgtcgatg catagtaaaa atgctcgccg cttttatccg tgagaaagaa
    17941 aacataattc gtatcggcag gatgaagcgc agcatttatc gctttaagtc ccgggttgca
    18001 gatcggaccg acaggcaaac cttcacgata atatgtgctg taaagctcca tatatttttt
    18061 tacttcatca ttctgatttt aaagtgccac tccgtcttga taaccgataa catctttaat
    18121 attctgcgaa acatagaatg tcgtaacgtc gctttcgagc ttcggaaacg atgcgggatc
    18181 cttgagcctg ttatggaaaa cagacgaaac attggcgcac tcgtcaatgt cggcatcgca
    18241 ctccttttga attacggaag cgagtgccat catctgatcc atagtgtagc cgagcgtctt
    18301 tgctctctgc ttcatttcgt cggtaatttt ggaattgtag ttttcaataa atttgtcaac
    18361 gacagaccga gcatcggact caagataaaa ttcgtaagta tcagggaaaa tatatccctc
    18421 caagaggaaa actctgtcgt ccgcattgtc aagctcaatt cccgcagcag gcttcttgca
    18481 ggcctcgaga aacgcctgag ccgaacaaac tttgctttgc tccagctttt gtgctatctg
    18541 aactatcgtg taaccctccg gaaatgtaac cgtcacggta ttatccggca gggtggtttt
    18601 gctttttgtt gacaaaaaag gaatcccttc tcccggatta cacgccgcaa aagaaaaaaa
    18661 cataacggcg cagagcatta aaattgcgat ttttttatat agcttcattt gtatacctca
    18721 tgtattcggt ttttgtcatt ttcgtatatc atacatcttt ttcgtttcta tgtcaataaa
    18781 agtgtacttt ctgcaaatat tattgttgga ggtgattttt atgaataaga aagagcgcaa
    18841 aaagaaaaaa gagttgaaac tttcgccgtt ggtacagtca atcgtcagcg attgggaagc
    18901 gggcagtgac accgatgttc tcggcagcta tacgggcaac tcgacggaaa ccgtcaagcc
    18961 gcagcaagac gcagacgatc tttaattgac attttaatga atataatagt ataataaatt
    19021 caaaagaaaa ccgctttctg cggcgcaaaa aatcatttcc ctgcgtcgca gttagtggtt
    19081 caaaatcaca atcaccgcta catattgata cttacaaatt ttattgccgt tttacattat
    19141 aataagcaaa atttgcttga caatgccggc atattgtgat agaataactg tacctcttta
    19201 aagagggttc tttttttgtg ccctctttta ggaacttaca gggtgtaggt tcccacatga
    19261 gggaggctta aaatcccgga caaaccggtt aaatggaggt gccgaaatga gagttaaggt
    19321 tacactcgct tgcacagaat gtaagcagcg caattacgac acaatgaaaa acaagaaaaa
    19381 cacacccgac aggttggaga tgaacaagta ttgcagattc tgcaagaagc atactctcca
    19441 caaagagacg aagtaagccg gaaggagaac tgttatggca aagaaagaga gttccgaagc
    19501 tgcaaagaaa atcgcaaagg ctgaaaaaga aaagacttca aagaagccga gtaataagcc
    19561 caatgttttc gtgcgtatgg gcaaagccat aaagaatttc ttcaagaaca gcaagggcga
    19621 tctcaagaag atcacatggc ccggtgcaaa ggaagtcctc aagggaaccg ttgttaccat
    19681 cgtttgcata gcaatcgtcg gcgtacttgt attccttgtt gacctcggat taactaaggg
    19741 catcagcgga cttcgtactg tcgcagagaa ccgcacaact acaactgttg ccgagaccac
    19801 gaccgcttcc gaaaaggaaa ccacaacggc cggaatcaac gtagtgaccg acagcagcac
    19861 aacagcggct caataatttc agcgagacgg aggaacggca atgtcaaatg atgccagatg
    19921 gtatgttgtt catacctact cgggctacga aaacaaagta gccacaaacg ttttgaaagc
    19981 tgccgaaaac cgcaaaatgc aggatgagat ctttgaggtc aagattccta ccgaaaccgt
    20041 cgtcgaagtt cgtgaggacg gtaagacccg cgaggttgaa cgaaagctct tcccgggtta
    20101 cgttatggtt aaaattgcaa ctttccttga tgaaaacgat atgccgaaaa tcagcgatga
    20161 agcgtggctc gttatccgca acacaagagg tgcgacgggc ttcgtaggtc ccgagggcaa
    20221 gcccattccc ctgacagagg aagaagtcta cagactcggc gttgaaaaga aggtcgttga
    20281 agtcaagtat gaggttggcg atacagtcaa catcaccgat gaaatctttg aaggcttcac
    20341 cggtaaggta gaggaaattg accttgacaa taataaggtt aaggttacgg tttccgcatt
    20401 gttcggtaag gaaactctgg ttgagcttga gcttgatcag gttgaaccga tttccgactg
    20461 atgaaacaag ataattgagc tgaaaggctt gatttatatg tcccgatggg gcatggagct
    20521 tcggctccgt tgtgggagga gcaaaaattt taatcaaatt gctccgacga accacattat
    20581 tttggaggtg caagaaatgg ctcagaaggt agtaggcttt attaagcttc agatccctgc
    20641 cggcaaagca acaccggctc cgcccgttgg tccggcactc ggacagcacg gcgttaacat
    20701 tatggcattc acaaaagaat tcaacgagcg tacaaagaac gacgcaggtc ttatcatccc
    20761 cgttgttatc acggtttatg cagaccgtac ttttactttc gttacaaaga caccgcccgc
    20821 agcagttctt atcaagaagg cttgcggaat cgagagcggt tcaggtgtgc ctaacaagac
    20881 aaaggttgcc acaatcacaa gtgagcaggt tagaaaaatc gctgaacaga agatgcctga
    20941 tttgaatgca gcaacgatcg aaacagcaat gtcaatgatc gccggtactg cacgcagcat
    21001 gggcgttact gttgtagact gatgctcccg cgtgggaggt aaaatccgat ttaaccacta
    21061 taatgggagg aatatcttat gaaacacggt aagaaatatt tagagagcgc aaagctcgtt
    21121 gacagagcta cgctttatga tccggctgag gctcttgatc tcgcagtcaa gacttcttcc
    21181 gctaagtttg atgaaactgt tgaaatccac gttcgtctcg gtgttgactc tcgtcacgct
    21241 gaccagcagg ttcgtggtgc tgtcgttctt ccgaacggta caggtaagaa ggtaagagtt
    21301 gctgttttcg ctaagggcga cgcagcagac gcagcagctg ctgcaggtgc agaaatcgtc
    21361 ggcgccgagg atcttgttgc taagattcag ggcgaaggct tccttgattt cgacgtcgct
    21421 atcgcagctc ccaacatgan nnnnnnnnng ctgttgaaga ggctaaggcc ggtaagattg
    21481 agtacagact tgacaagaca aacattatcc actgcccgat cggtaaggtt tcgttcggtg
    21541 ccgagaagct tcaggagaac tacaagactc tccttgacgc tatcaacaag gcaaagccgg
    21601 ctgcatcaaa gggtcagtac atcaagagct gcgttgttgc tacaacaatg ggccccggcg
    21661 tacgcatcaa ccctgcaaag gctgttattg ctgaataatt caaacaaaat aaaaaggtcg
    21721 ggcattgcgt ctgacctttt ttgttttatt aaattcgaga gttacgggag tgaagtaaat
    21781 gaaggccgct aaaaattccg ttattctttc ttctgttcaa tatatcataa tcttcatagg
    21841 ctttgcactg tcatatatgt tcacctatcc gcagtatgac gttctgcttt tcacaagaga
    21901 tttcaaaggt gattttgtca gcatcttgcg tgaggcgctg ctttacggca atggcaggtt
    21961 tttaggcaat gtgcttggct tttattattc gcatcatttc acggcagcga ttttcgtcac
    22021 ggcatttttc ctgacactga ttgttttcct tgcaaataaa ttattttttg acggaaacaa
    22081 aaaagcgata tttccgattg ctgtattgat tgcgttccct gccgtcggcc tgattcgtga
    22141 ggcatacagc atgattgccg cattctgcaa ctatgttgtt ccgctctcat ttgcgttaat
    22201 gtctgggatt tttataaaaa aactgaaatc aagcggttca cgttttattt atattccgct
    22261 cgccttttcg gcaatcgggg tatgtctttt cagtgagaac acgactatcg ttatgctctg
    22321 cgttgcagta ctgattattg ccggagactt tataagcaat aaaaaagcgt ctgtggcaag
    22381 cattatgaat ctgattttta cggcaatcgg cacattgata atgtatctca tcccgagagt
    22441 gaccgacacc aaagaaaagc ttgactatta cagaggttat gttgaaacgg actctttgat
    22501 tttcaatata acatcaacgc tgaggagctt tgctcttttg gcaaatcaat atgctcttct
    22561 ttatgttata atttccgccg ctctcattta tgtgctatac aaacagtgca aaacgagcaa
    22621 aattataaaa tttgttctgg ccgcaattct cgcagtcttt ccgctaacgt gcgtttttgc
    22681 tttgattttt gcacaaaaag caaacgagct ggcagcatta tatttgccgc ttgaggcaat
    22741 ctatttgatc gccgctttga ttgtttcaat tctctcaaag caaaaaaaat tgatagcctc
    22801 ggttatcatg accgtcattt tgctcggcag tgcagtcgct ccgatgacaa tagtaaatca
    22861 caggggtgac agaacatttt ttaccacgtt tgctatcctt ttctgctacg ctttgtactt
    22921 aacaaaagaa atcggagtag atataaaagt gaagcgtata ataaacattg ccgctcccgt
    22981 tgcttttgcc acaggctgct gcgttatgct cacactcacg gcgcataact ttgattcata
    23041 tgcctacaga gccgactatc tcgcacaaaa aagtgtgact gacagcagtg ccgatgtgcc
    23101 gtatctcgcg cacggccgtc tcgctcagga gacagcgctc ggtcagattg acccctgcct
    23161 tttcggaagc agtgttcaaa ttagcttcaa cgttatccca tcggacgaat gggaaaaagc
    23221 agacgaatac aaggagataa cgagcctgcc gcctgttgcc gcagtcaaat acggacttga
    23281 gcattgggaa tttaaggatg caacctatcc gcttaagctt cacgaaaaat attcgcagaa
    23341 caagtaatca atattatttt ttcaatatgc agtaaaaaag gctgtcaatt tcggttaatg
    23401 cttatgcgcc gaatttgaca gccgtaattt tattctgtta tcggcatctc tgtcatgcga
    23461 agatttgtac agccgtacgg aatgagctcc gtttcaatcg gttcaccaat tgactttttg
    23521 ctctccggaa gctcctcgca tctgccgtcg gtcatcttcc aatcgacatg tgccattttc
    23581 gctttaagct ttatcggtgc tccgtctggt gagaacggga catcgcctac ctcgccgaat
    23641 tcaacggtga atttcttgtc ggcaaagccg taattccaat ccgtggtcgg gaaaa
//
LOCUS       DAWBSW010000007        22796 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_483_length_22796_cov_3.099116, whole genome shotgun sequence.
ACCESSION   DAWBSW010000007 DAWBSW010000000
VERSION     DAWBSW010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 22796)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22796)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22796
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_22796_cov_3.099116"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4140..4149
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5099..5108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9061..9070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9787..9796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13511..13520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17854..17863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18664..18673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19754..19763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21396..21405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6283 a         4961 c         5019 g         6443 t
ORIGIN      
        1 attttatttt tttcttttaa aaggtcgtat gaaataaatc ttgtttcaag aaggtcaaac
       61 catttttcat gccaaagctc tgtgaaactg acactttttg tattattaaa ataaatatac
      121 aactcggcat caataaaaat aaacttttct gcttttgaat aaatctcaga attaaaaacc
      181 gtatcttcac cgattttgta ttttttaaat ttaatatcat ttaaaaattt ttttgaaatc
      241 aattttgtac atgccgagga aaaaacaagt tcattattgt ttgtaaattc agaatcgtaa
      301 aagcttataa aattttcagg tttaaaagtt tctgttttat attcaaattt tttctgaata
      361 aattcagatg tttttttata tcgacaaaca gaaaaatcgc agttattctc cgtaacggct
      421 ttgtaaagaa attcatacat ctgcggctga ataaaatcat cggaatcaac aaatccgata
      481 taatcgcctt ttgcattttc aatgccgaaa tttcttgcca atgaaacgcc tttattagga
      541 tttttcatcg gaataattct gctgtctttt tcagaatatt tttttataat atcaaatgtt
      601 ttatcgttac ttccgtcgtc aacgcaaaga acttcaaaat cggaaaacgt ctgattcata
      661 acagagtcaa gacaacggga aatatttttt tcttcgttat atgaaggaat aattatgctg
      721 attttaggag acataataat cacctgttga tcttttttat caggtcggca agcggatcct
      781 tgttagttgg ttgattatca tttgatttgt aaattccgag tctgtatttt ctgcctgtta
      841 cgtcaaaaac cgctttacga attgccattg aatgattgct ctgacgaatg aactgtgaaa
      901 ataccggatt gtcacattta atgagaataa aatctcctct gacatatgcc gttgaagccc
      961 cgagaatccc tgtaagaggc ttatctgaaa catcaaggag cgaaagcacc tcaccccatt
     1021 ggttaaaaag cgaatcctct tcggtatcat tgggagtttc gtttgtcgga agatcgctgt
     1081 gaatttcagg cggagtgtcg gaagaatcag aagctttttc ctgtataata ggctgagaag
     1141 ttttaacttc ttctttaaaa ctctctgtcg gtttttcagc agaaaatttt ccgcttttaa
     1201 taattctttc aaggttatca attctgctca taacggcagt attgtcactt gaaatattcg
     1261 gcacagtcat tttaactgcc gtaagttcca tttcagtgcg cttgcttgag ctggttttta
     1321 acacagttga agcttcttca agcaaagaaa tgcagttaag tattttataa agattcgttt
     1381 tgtcagcttg ttttttataa agctcaagat cgtgatccgt acatacaatc agtttttgcg
     1441 gttcctttac cgtcttaatc atcatcagat ttctgtaatg attgataagc tctgcacaaa
     1501 gtctttccat atcgcaagat tctccgtgaa gcttgtcaat tatcgaaaga acctcagacg
     1561 aattattatt atttatattg tcaaccattt caaaaagatg gcctttacct gcaaggcctg
     1621 ctgcattgct gacaatatct gccgtgacat ggtcgctgat tccggcacaa cggtcgagaa
     1681 tcgaaagtgc atcacgcatt gcgccgtcgg caattcttgc aataagaagg gaggcatcat
     1741 catcaagctc gattttttct tcccctgcta catatttaag tcttgcgctt atattttcag
     1801 gtgtaattct gtgaaaatca aagcgctgac atcttgaaag aatcgttgcc gggagcttat
     1861 gaacctcggt agttgcaaga acaaatttga catgctcggg cggctcttca agagttttga
     1921 gaagagcatt gaatgcgccg attgaaagca tatgaacctc gtcgattata tacacacggt
     1981 attttgtatg aacgggggta taatttgctt cctcacgaag gtcacggata ttgtcaacac
     2041 cgttgttgct tgcggcatca atttcaataa cgtcaagtat cgaaccgtcg tcaataccct
     2101 tgcatatttc acactcattg catggattgc cgttgtgggt atgcggacag ttgaccgcct
     2161 tggcaaatat ctttgcacag gttgttttac ccgttcctcg tgagcctgtg aaaagatagg
     2221 catgagaaac cttgcctgat tcaatttcgt gggcaagagt tgaggtaata tgttcctgac
     2281 ctataacgtc ggaaaaagtc tgaggtctcc actttctgta aagcactctg tacacgtcat
     2341 atacctcctc ttgtaataat aaaaagcatt atctcagtca catggtgtgc aggccgtact
     2401 gtgtcgcacg gtgttaaccg cttaatgctg ctcggttccc cgcctgacat ggttcgcggc
     2461 accccgtcgc acaggaccaa cacaccacat gactgaaata atgcttcaga ttaaatctcc
     2521 tgcaatatct taattattat aatatatttt cggtatttta gcaagggatt ttataaaaaa
     2581 ttattcattt tctttaggtt ttttcttttt aacgacgtat ttttttatca gcagaacagc
     2641 aattactatc ggaattgaac aaatcgccgc aaggacaata tatttcatcg gaataaggtc
     2701 gtaaattccg tcgccgagaa tcgtaaaagc aataactctc ggtgcaattc cgaggactga
     2761 aagcaaaagg taaggcaaaa atttaaaatc aaacgcaccc atgaaaagcg atgaaaaatc
     2821 aataggaaag gccgggataa aacgcatcag aaaaacagca acatttcgat ttttatcaag
     2881 cattgagaaa aatttatcac cggcttttgt gttgcggatt ttcttttcaa cggcgtcctt
     2941 gccgagaaat ttgcccatga aaaatgttac cgtgacctca atggcaatgc cgagaagatt
     3001 gacaataacc gcgcgtttgg tgtcgaaagc cataccgacg gaaatataga tgagtgatgc
     3061 gggaatgacc attaaaacag ctttgacgga ataaacaccg agtatgatca actcggcaat
     3121 caataatgat gacgcacctg caatcagtac acggacatcg agattcgaaa gataatcgta
     3181 gttgactatt gccgtaacaa atatggcgac agcaaatacg atttttatta tatttttaac
     3241 ggtttctttt ttcattggct tctccgaaaa tgaaatttta taatattata ccacactgaa
     3301 tgcaaaaaga aaacgaaagc cgaaaaaaat tcgactttcg cttaactcaa taccgcataa
     3361 ttgagatgta ttgacttagt attttatttt tatcccatct tttccttgat gtgtgaaagt
     3421 ctttcgagag cctcattgag ggtttcatcc ttctttgcaa agtgaacacg aacgatatta
     3481 ttgatgccct tttcgttgaa gaaagatgta ccgggaacgc aagcaactcc gactttctga
     3541 gccatttcaa ggcagaaatc aacgtccgac tgacccggtt taaggaacgg ggcaatatct
     3601 gcaagaacaa agtatgcacc ttgcgggtct gtatacggaa ttccgatacg gtcaagaccc
     3661 tctgtgaaga gctttttcat atgagcatag tgtgcaccga actcttcgta atagctgtcg
     3721 ggcatattga gaccgacgca ggcagcctcc ataagaggag aagccgcacc gacagcgttg
     3781 aagtcgtgat actgttttat cctgtccatg agcggcttag gagcaattgc atagccgaga
     3841 cgccagcctg ttactgaata tgtcttggaa agagaggaac aactgactgt tctctcccac
     3901 attccgtcga gactgttcat ataaatatgt ttatgtcctt cataaatgat gtgctcataa
     3961 acctcgtcca tgatagcata aacatcatac ttgatacaaa gatctgcaat ggttctcagc
     4021 tcgtcgtatg tgaaaacctt gccgctcgga tttgaggggt tgcagataag aattgctttt
     4081 gcgccttttt taaattcagc ctcgatgata tcgggatcaa agttaaactc gggcggaacn
     4141 nnnnnnnnna aacaggctca cagtctgcca taatagcctg cgctctgtaa ttttcaaaat
     4201 agggagagaa cataacaatt ctgtctcccg gatttgtgag agcgaaaatt gtatctacca
     4261 ttgcttcggt tgaaccgatc gttacaacca tatctgtatt tggatcaagc tcgcgaccca
     4321 taaaacgtcc gcactttctt gcgagagcct cacgaaaatt cggtgctccc attgtgatgg
     4381 gatactggtg agggcctgta tgagcaacct ctgcgagacg atccgtcata gcctttggag
     4441 gatcaaaatc cgggaagccc tgtgcaaggt tgattgcgcc gcagtcaaga gcaacacggg
     4501 tcattcttct gataacggaa tcggtgaaaa gtgcattttt tctgcttaat tcctgcattg
     4561 ttgtatattc ctttcttaac aaaccgactg aaaggaaagt cgatttattt tatttgttta
     4621 gatttatttc tcctttgtta aattcaacgg aacaaagaag agcgacagaa aagataaagt
     4681 ttatttaaaa gcatttatct gtttcaccgc aaaaggtccc cctccccttg aaataagagg
     4741 agacagactt gtttttaatt taacggcata taaataatac tccgtcagtc cgaaaaaatc
     4801 aatagttatt taattccgag atcgtcgccg gttgcaacaa cgacaaagta ttttttgaga
     4861 acagacgtga aaatatcaac atccttgacc ttgaaaatca tgtatgcaag accgtctttc
     4921 tgtccgaaaa cggaatacat gtattcaatg tcaatatcct cgtttgaaat gatattgaga
     4981 agctcgttga ggccgcctgc tttattggga acggcagcag caacaacggg agtttgcgta
     5041 acaataaaac cgttattacg aagaagcttt gcagcttcat tgcaatcgtc ggaaatgann
     5101 nnnnnnnncg aagaattccg taatcggcgg tctcggcgat gttcagcgcc ttgaggtcga
     5161 tgttgttatc ggaaaggatt ttcgtaatct ccgaaagctg tcctgctctg ttttccaaaa
     5221 aaaccgaaat ctgagtaata gccattgtcc ttcctcctta tatctttctc ttgtcaataa
     5281 cacggactgc cttgccttcg cttctggtga ttgacttcgg agcaacaagg cgaaccttgg
     5341 tgtaaattcc gagcatagcc ttcaaagcgg caacaagtga tttttccatc tcagtaatct
     5401 taccgaggtt atctgagaag ttgtcctgag tcatttcaac cataacttca agagtatcgc
     5461 tgttgtttac acggtcaact atgatctgat aattagactg atagccctgc tcaatgagaa
     5521 cggtctcgat ctgtgacggg aatacgttga cgcccttaac gataagcata tcgtcgcttc
     5581 tgcccatcgg cttgcacatt ttaacaagag ttctgccaca ggagcatttt tctcttgtaa
     5641 gaacgcagat gtcacgggtt ctgtatctga gaagcgggaa tgcttcttta gtgattgatg
     5701 tgaaaacaag ctcgccctga gttccgtcgg gaagaacctc acctgtatcg gggtcgataa
     5761 tttcggcaat gaaatgatcc tcgttaatgt gcatgcctgt ctgatcggag cattcaaatg
     5821 caacgcccgg accgctgatt tcagtcagtc cgtaaatatc atatgccttg attccaagct
     5881 gattttgaat ttcctgacgc attttctcac tccatgcttc tgcgccgaag atacctgcct
     5941 tgagcttgat tttatctctt agaccgcgct cgtggatact ctctgcaaga tatgccgcat
     6001 aggacggagt acagcagaga attgttgact gaaggtcggt catgaactga agctgcctgt
     6061 ccgtgttgcc cgaggacata ggaagagtga gactgccgac cttatggctg ccgccgtgaa
     6121 gtcctgcgcc gcctgtgaaa agtccgtaac cgtatgcaat ctgaacaaca tcttcattcg
     6181 ctccgcctgc cgcaacaata gcacgtgcac agcagtcctc ccagaggtcg atatcattct
     6241 ttgtgtagaa ggcaacaaca cgtctgcctg ttgttcccga ggtggactga attcttacgc
     6301 agtcctttaa cggagcggca agaagtccgt agggataagc atcacgaaga tccgcctttg
     6361 aaaggaacgg aagcttgtga agatcgtcaa cacccttgat atcgtcgggt gtaacgcctt
     6421 tctcctccat cttctttctg tagtaaggaa cattgtccca aacatgcttg acctgcttaa
     6481 caagtctttc gtcctgaagt tcacggagtt tttccggtga ggcacattca atttctttct
     6541 gatagtaatt ttccataacg aggtctccta tttatgcgtc tttgccgagc aagaaagctt
     6601 ttttgtttat ctcaaggaac ttaggaggaa cgcttttttc aatcgctgcc atccattcct
     6661 ctgttgtaaa gtcaaaatac tttgaaagtc tgcccatgag aacaaggttg accgctttgg
     6721 aagttcctgc ctcctcggca agtgaaagag catcaattgc gtcaagatta acaccgagag
     6781 ccatgatttt tttaacaagc tcggtcgggt attctgccgc accggtaata accggcatag
     6841 ggttaatttc ctgagtgttg gtaactatag ttccgtcggg tttgagaaat tcagtccatc
     6901 gagccgcttc taacagctca aaggatataa taatatcagc ttcgcccttg tcgataatcg
     6961 gagaataaac cttgtcaccg tatctgacat atgtaacaac actgccgcct cgctggctca
     7021 ttccgtgaac ctcgctgacc ttaacgtcgt agcctttttc aagaagaagt ctgccgagaa
     7081 gcttgcttgc aagcaaggtt ccctggccgc cgactccgac aatcataata ttcttcgtcc
     7141 gcataatcag gactcctttc cgttcggaag tgcgccgaat ttgcagagct gactgcaaac
     7201 gccgcatccg gtacaaagtg ttgcgtcaat cttaaccttg ccgtcaacaa tgctgatggc
     7261 aggacagccg atcttcatgc aggatttaca accgatgcac ttgtccgcat ttgccgtaat
     7321 cggtccgtta tgtttaacat atttgagaag tgcgcacggt cttcttgaaa tgataaccga
     7381 aggttcgtct gccgcaagct cctctttaac tgcgtcgtca cattctttaa ggttatacgg
     7441 atcaacaact ctgactctct tgatgccgac cgaacggcaa agggtttcaa ggtcgatctt
     7501 tgtgcacggg tcacccttga ggttgaagcc tgttgtcggg ttctgctgat gtcccgtcat
     7561 gcccgtaatg gagttatcaa gaatgataac ggttgaattc gattcattat aagcgatatt
     7621 gataagtccc gtaacgcccg agtgcatgaa tgtggaatca ccgataacgg caaccgtttt
     7681 tccgtcgctc ttgccttcgt tagccttgtt aaagccgtga attccagaaa cggaagcacc
     7741 catgcaaatg gttgtgtcaa ttgcggcaag cggcggaact gcaccgagtg tgtaacatcc
     7801 gatatcaccg aaaacagtga gcttattctt tttaagcgta tagaaaaggc ctctgtgcgg
     7861 acatcctgca cacataaccg gaggtcttgc aggaatgttt tcgtcaagct tctttccaca
     7921 gtcgggggta aatccgagct tctctgcaat gatgttctga ctcagctcgt caatacaact
     7981 gaacagttcc tttccggtta cctcaacgcc gatgttgcgg cagtgagttt cgattatgcc
     8041 gtcaagctcc tcaataacaa ttaccttgtc aaccgacttt gcaaaatcct gaattttttt
     8101 gaccggcaga ggatttatca taccgagctt gagaacggga tatttatcgc cgcaaacctc
     8161 ttttgcgtac tgatagcttg tgcttgatgt gatgataccg attgaatgat cgcttccgtc
     8221 ctcaatgcgg ttgaaaggag ctgtttcggc atactctgta agagccgccg ttcttgcttc
     8281 aacgatcgga tgtcggcgct ttgcgttgcc cggcatcata atatattttg cgccgtcttt
     8341 ttcgtaggtc tttgaggtct caactctgtc cgatgtttcg ataatgcttt gagaatgagc
     8401 aactcttgta cacatcttga caaaaacggg tgtatcgtat ttttcggaaa tgtcgtatgc
     8461 gagcttcgta aagctgaggg attcgcttgc gtccgccggt tcaagcatcg gaagctttgc
     8521 ggcaatagcg taatgacgtg aatcctgctc gttctgagag ctgtgcatac ctgcatcgtc
     8581 cgcaacgccg ataatcatac ctgcgttaac gccggtatat gcgattgtga aaaccgggtc
     8641 ggctgcaacg ttgagtccga cgtgcttcat tgaacagaag cttctcttac ctgcaacgca
     8701 tgcaccgaaa gccgcctcaa cggcaacttt ttcatttggt gcccattcgg cataaatttc
     8761 cttatacttt gcggcatatt ccgtgatctc ggtactcgga gtacccgggt agctggaaac
     8821 aaaggaacag ccggcttcgt aaagtcctct ggcaactgct tcgttgccga gcatcaactg
     8881 cttcattata taaacctccg tttgctttta gcctgtaatc tgtgattcga caagtctgcc
     8941 cttgcaatac tttgccctga gtacaggctt ttctatcttt cctgtcgggt tgcggggaac
     9001 tttttcaaat ataatcttct taggacgttt atatctcgga agctcctcac agaaatcgtt
     9061 nnnnnnnnnn gcagtaatct caccgagtct gggatcggga atacctataa cggcaacgtc
     9121 cttgatttta tcgttacggc ggaggaagtc ctcaatttga acaggataaa tattctcacc
     9181 gccggaaata acaacatcct ttttacggtc aacaagatag ataaatccgt cctcatcctc
     9241 tcgtgccata tcaccggtgt agagccaatc gcccttgaga atttcttctg tcgccttcgg
     9301 gtttttgtag tattcgagca taacgcccgg tccttttacg gcaagctcgc cgacttcacc
     9361 ctgcttaacc ggttcgccgt ggtcatcaat gatttttgta tcccaaagat agcctgcctt
     9421 gccgattgcg ccgaccttgt taatgttttc aacgccgaga tgaacgcagc ccggtccgag
     9481 agactcgctg aggccgtagt ttgtatcgta ctgatgatgc gggaaaatct ttttccatct
     9541 gtggataagg ctcggcggaa caggctgtgc accgatgtgc atgagtctcc actgatcgag
     9601 cagataatgg tcgagattga ttctgcccga atctattgca tccagaatat cctgcgccca
     9661 tggaacgaga agccaaacaa ttgtgcattt ctcctccgta attgtgcgaa gaatccattc
     9721 gggcttaatt cctctgagga taacggtttt actgcctgag agcaagctgc cgaaccagtg
     9781 cattttnnnn nnnnnntttt gctcctgtgt gataaagcgg aggaatgcag aggaaaacat
     9841 cgttcttttg ctgtccgtgg tgtttctgtt cagtcaggca cgaacaatag agagccttgt
     9901 ggcgatgaag aatcgccttc ggaaagccgg ttgtgcccga tgaaaaataa attgcggcat
     9961 ggtcgttttc gtccaaagct atcggcggaa gcgacgacga gcaatagcga ataagagtca
    10021 tgcagttttc tgcatattca ggagtatttt tgccaacaaa aagagtaatt ttaatatttt
    10081 tcagttcatt gtgtatcggg tcaactcggc tgatgaattc agggccgaaa ataaaaacct
    10141 caacatccgc aagctcgaga caatatttaa tttcatcgcc tgagtatcgg tagttcatcg
    10201 gcacggcgat gcagcctgcc ttgagaattc cgaaatataa cggaagccat tcaaggcaat
    10261 tcataagaag aattgctacc ttggtgcctc tttttacatt tctgctgagc agaagatttg
    10321 caaatcggtt tgccctgcgg tcaaagtctt tccacgaaag ctcacggcga tagggtgcgc
    10381 cggggcttgc actttctata agacttgctt cacgccaggt tacggcgttg tcgcgctcaa
    10441 cggtggggtt aacctcaacg agagcggtgt ccgagggata aagacgagca tttctttcaa
    10501 gaagctcggt aataaccatt ttttccacct ctgattgttt taacttcccg tggtttcgga
    10561 aaattttatt ataaatgtat tatagaatac tttatcactt ttaaccgata aagtcaatgt
    10621 gttttaagaa aatgttgtcg gataaaagga catgataaac atgacagtat tataacatat
    10681 attatgctgt aatgtctata acatttgtgg gcgtatattg aacaggaaat tatttaaaca
    10741 caagctgaac aaggagcata taacaaactg tgccgcctgc gattgaaagc agcatctgtt
    10801 ttttccaaag gtggagaaga actacaacta taattgatat caattcaggc aaaccgtggc
    10861 ttcccgtgag aatatcaaca cttttaaggc aatagacaac cagcatcgcg aacacagccg
    10921 caggcaaagc cttgccgagg tactcaataa atttcggtgt ctgacgtttt tttgactgaa
    10981 aaacggcgaa cggaagaaat cttgttataa tagtcgccac ggcggttatt gcaatggtta
    11041 taacttgctg agtaacggtc attcaagtcc gccccctttc tcgataggct tgcgaagcgc
    11101 tgtaagcaga aaaagtataa gaagcattgt cggaatcata aaggagtccg agccgaaaat
    11161 gagcagacaa cttacagacg caaaaaggcc gataagcgac gtgtaatgtt tcttttcttt
    11221 catccactgt tcgaggaaaa ttacgacaaa cattgccgtc ataacaaaat caagacctgt
    11281 tgtgtcaaac ggaatgagtg agcccacaag tccgccgatg agcgaaccgt tgatccaata
    11341 aaggtgatcg agcagggtta cagcagtcat gaaccagccc ttgtcaattc cctcgggaat
    11401 atcaaccgag cagttgattg aaaatgtttc gtcgcacata ccgaagatca aataatattt
    11461 ttttatcccg acatttttgt acttatcaag cattgaaagt ccgtagaaaa gatgtcttgc
    11521 ctgaatcatc agtgacatta taagcgtttg aatcggtgca aaaggactca gcagcatcga
    11581 aacagcgaca aattcaattg aaccgccaaa aacaataatg ctcataacaa gcggatacca
    11641 aaagctgaat cccgaaacgt tcatcaaaac gccgaacgaa aatccgagga aagaaaagcc
    11701 ggcaaggatg ggtattgtgt gcggaaaagc agccttaaaa gcttcggcaa tcactttctt
    11761 ttttttcata aagtcccttc tcaacgtata attattcaca gtataataat tgtaggtctt
    11821 tgtcgttttt ttgtcaatgt caaagaaagt taaactttac aaagcaaaat tgtaattatt
    11881 atataaatag ctttaaattg tagcaaaaat tatgaatgta ttattataca aaaattctcc
    11941 cgaccgttaa aaaggtcggg agaaaaatta aatatgtttt tatttgttaa ccttaacgcc
    12001 gaaggtaact ttttcaccgt tgatgttcca ttctgcggta tgaccgtcgg cagagcctgt
    12061 aacgaggtcg tcggcaacaa cagcagtgaa gatatcagcc ttgttgcggg caatgatatc
    12121 ggcaatctta ccgttaccct caacgctgat gatgatgtga tcggtaacgt tgaagtccgc
    12181 atctttacgc attgtctgaa tcttgctgat aatttcacgg acaaagccct cttcaacaag
    12241 ttccggagtg agagttgtgt caattgctac agttacgccg aagtcggaaa ccgagaacag
    12301 acccgatttc tgaacagcct caataagaag atcgtcggct gtcagttcga ttgcaccgat
    12361 gctgatattc agcgtgatat gaccgtctgc gtcaagctct ttcttagcct ttgaaccgtc
    12421 aagctccgaa agcgcagttc tgatttcacc gagattcttg ccgtacttag gaccgagtgt
    12481 tttaagctga ggcttaaagt tgtaatcgga aagttcatct gcggcatcaa cgaagatgac
    12541 gttcttaata ttcagctcgt cctcgatgat atccttataa tagccctcaa gcttaatgtc
    12601 tgcattgaca tacatctttg cgagaggctg acggttttta atgtttgcgc cgtttcttgc
    12661 cgcacgtccg agacctacga tgttgagaac ctcgtccatg ttcttctcaa gctcaaggtc
    12721 aatatggttt tcgtttacct cggggaatga gcagagatgt acgctttcct cggcattctt
    12781 gtcaaccgag cgtacaaggt tctgatacat gtcctcagcc ataaacggaa tcatcggagc
    12841 agatgccttt gcaaccgtaa caagcgcggt gtaaagtgtc agataagcat tgattttatc
    12901 ctgctcaaga ccctgtttcc aataacgctc acggcagcgg cgaacatacc agttactcat
    12961 atcgtcaaca aattcctgga gagccttaca agcctcgggg attcgataat tgaaaagatt
    13021 gttgtcgacg ctctttacca ttgagttgag ctttgagaga agccacttgt ccatgactga
    13081 aagcttactg taatcaagct catactttgt cgggtcgaat ttgtcgatct ctgcataaag
    13141 aatgtagaat gcgtatgtgt tccaaagagt gcccataaac ttgcgctgac cctcaataac
    13201 agccttgcca tggaatcggt tcgggagcca cggagcggag tttgtgtaga aataccaacg
    13261 aatagcatct gcgccgtagg tcttgagagc gtcaaacggg tcaacggcgt tgcccttgga
    13321 tttactcatt ttctgaccgt tttcatcctg aacgtgaccg agaacgataa cgttcttgaa
    13381 cggtgccttg ttaaagataa gggttgaaat tgcaagaagt gaatagaacc agcctcttgt
    13441 ctggtcaaca gcttctgaaa tgaaatctgc cgggaactgt ctctcaaata agtctttatt
    13501 ttcaaatgga nnnnnnnnnn cggatagtga tgctgagcga aaggcattga gcctgaatca
    13561 aaccagcagt caataacctc aggaacacgc ttcatctgct tgccgcactc gggacacttg
    13621 attgtaacag cgtcgatata cggacggtga agctcgatat cgtcaggaca gttgtcggac
    13681 atgctcttga gttcctcaat gctgccgatt gaatgtctgt gaccgcactc acattcccaa
    13741 atgttgagcg gagtacccca gtaacggtta cggctgatgc cccagtcctg gacattttcg
    13801 agccagtctc cgaagcgtcc tttgccgata ctttcgggaa tccagttaat tgtattgttg
    13861 tttctgataa ggtcatcctt aacagccgtc atcttgatga accaggactc tcttgcatag
    13921 taaataagcg gagtgtcaca gcgccagcag aacggataat catgttcaaa tttcggtgcg
    13981 ctgaaaagaa gtcctctctc ttcaagattc tgaagaacgg gaacgtccgc atccttaaca
    14041 aagagacctg caaacggagt ttccgctgtc atgttgccct tgccgtcaac gaactgaaca
    14101 aacggaaggt catagtttct gccgattcgg gagtcgtcct caccgaatgc cggcgcaatg
    14161 tgaacgatgc ctgtaccgtc tgacattgta acgtagttgt cacaggttac gaagtgagct
    14221 tttttatgct gctgttccgc ttttttgccg gcgcactcaa agagaggctc atattcttta
    14281 tattcaagat ccttgccgac gaattcttca agaatttcgt aagccggttt tccgtcctct
    14341 gcgagaggag aaagaacctt atccataaga gcaacggcaa tgtaataaac atttccgtca
    14401 acacacttga cctttgcata tttctcggtc ggatttacgc agagcgcaac atttgaagga
    14461 agggtccacg gagttgttgt ccatgcaaga agataagcgt cctctccctt gaccttgaag
    14521 cgtgcgattg ccgaacgctc tttaacggat ttatatccct gggcaacctc atgtgaagaa
    14581 agcggagttc cgcagcgagg acagtaggga acgatcttga agcccttgta gatgagaccc
    14641 ttttcgtaaa tctttttgag cgcccaccac tctgattcga tgtatggatt atggtaggtt
    14701 acataaggat tatccatgtc tgcccaaaag ccgacagtaa aagagaaatc ctcccacata
    14761 cccttgtatt tccaaacgct ttccttacat tttttgataa acggttccag accgtactgc
    14821 tctatttgct ccttgccgtc aagaccgaga agcttttcaa cctcaagctc aaccgggaga
    14881 ccgtgggtat cccagcccgc cttacgcgga acctgatagc ccttcattgt gcgataacgc
    14941 ggaatcaggt ctttgataac acgagtaaga acgtggccga tgtgcggctt gccgttggca
    15001 gtcggcggac cgtcatagaa aacataggac gggccttttt tctcgcaggt tttttcaaag
    15061 attttgtttt ccttccagaa ctcctcaacc gctttctctc ggtcaacaaa gttcaggttt
    15121 gcagaaactt tcttgtacat ttatgatagc tcctttttgt gtgataggat aatgctcaaa
    15181 acaaaacccc cgtcccgtga aaaacaggac gaggatgact cgcaaccacc tattcaacat
    15241 acttcaatat gtgttccctt taacggagga cacccgtcaa aacttactca attcagtccg
    15301 cagctcgaaa gtgatattca ccgcaatgta cttcacgccg ggcttacacc ctccccggct
    15361 cgcttttgct tcgcatagcg gctactgtct ttgtcacagc ctttattata tcgcttataa
    15421 gatatcacaa aaccattttt gtgtcaagga atattttgtt tcattttggg tttattatat
    15481 atataatgta ataggagacg aaaaaattta tttagacaac ttatcttttt tgttaaaaac
    15541 ggcattaaaa ttgtattttg cacagtttta accataatat ttagtgactt tttcaaaaat
    15601 agataaaatt tttctccaaa ggcaaaaaaa tacttgcaaa atgacgggcc aacgattata
    15661 attcatagtg aggggagtat ttgccagggt tgctgtcttc gggcagctca actttataag
    15721 cggtactaac gttataccac tttaaagccg tggaaaagat gctcaaacct caaaaaatta
    15781 aaggaggaat tgacatgacc aagatcattt caatcatcgt ttccattgta atgcttgtca
    15841 tgaccagcat tttcccgggc tttgtatggc cgggaacaaa gacggttgag acaggcgcat
    15901 tcttggagca ggttaatgaa gcgtttggtt atcaggcggt tgagtccacg gaagaaattc
    15961 gtggagttaa gccggacaac gagtattatg cagctgttgc aacagcagat aataatgacg
    16021 ttctcgttga ttatgataaa atcgacgtta caaaggccgt tactccggaa ttcgtagcaa
    16081 cagttcttgt taatgcatcg ggcgttaagg ttgacgagac caccgttgta atcgagaatg
    16141 ctacatccat caaaaacatt gcaaaagttt ccgcagctgt tgacaatggc atcattaagc
    16201 ttgatgctaa gaacaagctc ggtctcggcg caatggcagc tgacgacgta acagcagcta
    16261 tcgcaaaggc tgttgagctt cgtggttcag ttgacgaaaa cacacagaag ctcgttctca
    16321 aggacggcgt taagacggtt tccgattacg ctgttgaggg tgacagcatc gttgttcccg
    16381 caagctcaga ccttgcagta ggcgacgttt atgttgttga gcagtctgat gctatcttca
    16441 caggtgcagc ttacaaggtt gaggctatcg acgttgtaga cggcgagaag gtagttaaga
    16501 attctgatgt tgctatcgag gacgttatcg agaagatcga ctacgaaggc tcatctgatg
    16561 ttgatttcgc aacatcaatg atcgctgacg gtaacggcga gattctttca gacggttacc
    16621 ttgatacaaa tgccatctct aaggatgaca tcatgaagac tctcaagaag cttgctaatg
    16681 ttagcttctc tgtaaagggc ttcaaggtta aggctaagat cacagataca ggcctcaact
    16741 tctcagttgc taagagcgta tgcgacggcg ttacactttc taaggcttat gagctcacaa
    16801 acctcacggt tgatgctaag gctgacatga acgttgcaaa gcttaacttc aacgaagtat
    16861 atcttaagtt caactatgac cttaaggata caacagctat ctcaggttcc tacgctaaga
    16921 ccttcggcga cgaagttaca tctgttggcg agaagctctc aggcgacagc ttctgggcta
    16981 aggctgttaa caagtatgct ctctcaaagc ttgattctac atcaatcaag ctcttcacct
    17041 tcactcttcc tctcggttca actccgctta ccattacatt cgacgttctc cttaacatcg
    17101 atgtaaacgg cagaatggaa atcgttgtaa cttctcagga aatgcacggt attgagatca
    17161 tcaacaataa ggtaagcaca gttaacgatt caacagttct cgatcgtcag gttaacatct
    17221 atggtcagtt cgagcttcgt ctcggccttg atgtatcact cggtctctat ggctacgctc
    17281 ttgtagacgt atgcgttgag ggcggcgttg gtgcttatgt taaggcacag gctaagtatg
    17341 ttgacgcaaa cggaaacgtt gtagttcagt catctcttac aattcctgtt gactacctca
    17401 aggagcttgc agcaggttca aacacagacg gtaagatcga tatcagcggt catgcggata
    17461 tctacggtat cctcaacgtc tctgttggta accacagcgc tatcggcaag gtaggtctca
    17521 agaagacatg gaacatcttc aatcagagca acggaacatt tgcttctttt gatttctgat
    17581 tttgatttcc gaatatcggc tcccgggaga aatcccggga gtttttcttt ttgtagattg
    17641 ggagcgcctt caaccgaagt tgaagacgct cccttttgtt gattattcgt gtggttgtga
    17701 attcatgaaa ggttatttca tgtttttctc ataggattca atcatctttt taaccatctg
    17761 accgccgaca gaacctgcct gcttagatgt gagatcaccg ttgtaaccgt tagaaaggtt
    17821 tacacctacc tcagatgcag cctccatctt gaannnnnnn nnncattctt cttgctgctt
    17881 gacatttgca tttcacccct tcgcaattca tttgcgtttg caattatatc ttttgcgtta
    17941 ttctcaaaaa tacaactgaa aaattttgta aacaagttgc catattttac atattgtgat
    18001 aaaatagttc cctgtgaact tatatttaat attatgttat tgtaagctgt tcagcatata
    18061 ctatcatcgg aggaattact ttggtttatt caaatgttct tgaagcaatc ggacacacgc
    18121 ctatgattcg cctttcaaaa attgtacccg agggcagcgc agaggttctt gtcaagtacg
    18181 aaggcgtcaa catcggcggt tcgattaaaa cacgaactgc atataatatg atctgcgacg
    18241 cagaggaaaa aggaataata cattcaaata cgattattgt tgagccgaca agcggcaatc
    18301 agggaatagg tcttgccctt atcggtgcag tcagaggcta tcatgttcgt attattatgc
    18361 cggattctgt cagcgaggag cgtcgaaagc ttgtcaaggc ttacggagcc gagcttgtgc
    18421 tggtccatga tggcggggat atcggcaagt gcatcaacga gtgcctgcaa accgcacttc
    18481 gtatggcgaa ggaggataaa aacgtttgga ttcctcagca gtttgagaat ccggcgaacc
    18541 cgatggttca taaaagctat accgcacagg aaataattga gcaggtcggc aagccgatcg
    18601 acggtttctg ctcgggtgtc ggaacgggtg gaactttcag cggaatcgga gagactctta
    18661 aagnnnnnnn nnnccgaaaa atgccgcaat tctttcgggc ggaagcatcg gaactcatct
    18721 tcaaatgggc atcggtgacg gacttattcc gaaaaatcta aacacacaaa tctatgagca
    18781 tattgatatc gttacagacg aggaagcgat cgaaacctca cgacgcctta tccgtgaaga
    18841 gggaatcctt tgcggaatat caagcggaac gaacgttact gcggcacttc gccttgcaaa
    18901 ggagctcggt gaaggcaaaa cggttgtgac tgttcttccc gatacaggtg aaagatattt
    18961 cagcacggtt ttgtttgatt gattttcagc gaggtgtttt tatgaatgaa aaagagcttt
    19021 gcaaagcggc aattgacgca atgaagaatg cgtatgttcc gtattccggc tacagggtcg
    19081 gtgcggcatt gatgaccgag gacggaaagc ttttcaccgg ctgcaatatt gaaaacgccg
    19141 catacactcc gaccgtctgc gccgaaagaa cagctttttt caatgcgatt tcttcaggcg
    19201 aaagagattt tgccgctatt gccgttgccg gcggcaagga cggaattata agcggagcct
    19261 ttcccccttg cggagtttgc cgtcaggtta tggctgaatt ttgctctccc gattttacgg
    19321 ttcttgttgt cacgggagag gagtcgtata aaaagtattc acttaacgaa ctgcttcccg
    19381 aagcgtttac gccgaaaaat attgaaaaat aaaaattacc gcaattaaag cctgccctta
    19441 tcaatgattt gaaagggcag gcttttgaat attttgacca aaaataaatg agttcttttt
    19501 tagtcaaatc tttaacaaac tcttgaaatg cgatgagttt taatatataa ttaaaataag
    19561 aatgtacggt ctcctcaaac gcaagcgacg gaggcttggt tcggaaataa taagtcctcc
    19621 gtgtcggggg cttatgagga taaaaggagg ataaacctta tgaatgaaaa cttcagctac
    19681 aaaatgaagc tgacagacga ggaaaaagaa attcttcacg gctcaaaggg cgaggtaatg
    19741 gcgaaggtta tgannnnnnn nnntttcgga gcagagaaat tcgttcccgt aaacggcaaa
    19801 ggacatcttg ttacaagctt cggcatttcg gttctcaagc ccgtgttctc cattatggat
    19861 gagctcatta agggcggcct caaggttgac gagggattta cagttgaccc gagacccatt
    19921 gactacgcaa acgttaagtg taatccgctc cagaagctga tcttcaacaa gattctctac
    19981 ggtatgcagg attcctacga ggctcagctt cataagcttg gactcaagag tgacaagtca
    20041 ttcacttgcg cctgctattt tgacgaggta ggcaacacac ccaagaaggg cgatattctt
    20101 tcatgggcag agagctcggc tgttgtattt gcaaactccg ttctcggtgc aagatgtaac
    20161 cgtaactccg gtatcatcga gctgttcggt tcaattctcg gcaaggttcc cgagtttgat
    20221 cttctcacag atgagggaag aaaggcaaag tggatcatcg aggttaagac cacaaagatt
    20281 ccggaggctc aggttctcgg ttcggcaatc ggtatgaagg ttatggagga cgttccttac
    20341 gtcaagggtc ttgacaagtg gatcggcaca gagctcacag acgatgccaa ggcatatctc
    20401 aaggatttcg gcgcagcaac cgcttcaaac ggtgcagtcg gactctacca tattgaaaat
    20461 cttactcccg aggcagttga gcagggcgaa agcctcatcg ccgagggtgc tcagacttat
    20521 gttatcgacg atgcagagct tgaaagagtt tacaaaaact atcccgttat gtggaaggat
    20581 aagggcgcag ctccgaagct ttgctttatc ggctgtcctc acctttcata tgcgcagctc
    20641 ggcgaatgga cagaaagatt tgagaatgag cttaaaaagc agggcaagac aaaggtcggt
    20701 cttcgtactg ttatgacaac cgctcccgag gttctcgaca gatttaagaa ggaaaacagt
    20761 gccgcttaca acaagcttat cggcttcggc atcaacattt cctgtatttg tccgcttatg
    20821 tatatgaaca atccgctctg cggcggcgac acgagcaatg ttattacttg ctccaacaag
    20881 cttcgtacat ataccaccgc acgttatttc acaactgacg atatcaccaa gatcgctgtc
    20941 aagggaggtt tttgattatg agtaagacat tcaaaggacg tcctgttgta gcaggcacaa
    21001 tcaataaggg cgaggctgtt gtttctcata acggtgtaaa cacacttgcc acattccaga
    21061 aaacagccct catgcatgat attccgatta tcggcaagga cgaggttatc ggttccgacc
    21121 agaataaccc ggatgtattc ggcaaaaatc ttaccggcaa ggttctttgc ctgcctcaga
    21181 caatcggctc aacaaccggc ggtatggttc tctacaccgt atgcgctctt aatatggctc
    21241 cggcggcaat gcttttctcg ctgccgatcg actctcttgc agccgcaggc gttatcctct
    21301 ccgacgtttg gacggataac actatcgttg ccgttgactc tctcggcgat gaattcctca
    21361 gcgctgtaaa gaacggcgac aagatcaccg ttaaannnnn nnnnnggacg gaactgttat
    21421 cgttgaataa aacctgactt taagagctgt tgcagaaaaa cggcaagttt ctctgctgca
    21481 gctctttctt ttcggaggaa tttatggcga atatcagaat agacaacgct tccgatgacg
    21541 tgctgaaaat cgagcttgac ggtgaggaat actttattcc cgaagacggg agtataacaa
    21601 tctcgcctgt tgaaaaagga aatcacaggc ttatcgcctg ccgtgcaata aggacggaga
    21661 cggataagaa attcgacagc gataaggata atccgacacg caagctttca aaggacgaga
    21721 acagccaata tgtgcagctt aagggagttt ttgaaataaa cgttatcgct tcaaaatccg
    21781 tttggacggt aaagaaaaag ccgatacttg ttgaaaaaac aggtgttgac gcactttttt
    21841 cgggctgcga gcttgagatc acgggaggca gtgttgttgc atcaagacaa agcttcgccg
    21901 atgaaaattt gcgcagggca tatctgaaaa aacagttaat gggcgctttt tttccgatag
    21961 gcatcggaat tttgattttt gcagagttgg ttctggttgc gctctcggca aacatttccg
    22021 gcaatccggt cacgctcggc ggcagagaat ttacatatcc gtggacattc ggtctggcgg
    22081 caattgacct cggctttata ggatatttta ccgcgatgct tataaatatt ttttctttgg
    22141 ataaaagata cagataatgg actgttgctt ttaggcgcag tccattatat tttcgtcaag
    22201 gttttatccg atgtgacaaa aatcgtcttt tatacaaaat aaagtgtaca atctcgccgt
    22261 aatatgttat aatagggcaa aatgtagaaa ggagcgcttt acagtgaaaa ggaaattttt
    22321 atcggttctt ttaattgttg caattgcggt cggtttttgt atgcctgttt ccgccgcaac
    22381 atcattgaag ctgacagacg caacatatcc gacgacatta aaacagggcg atccttttat
    22441 tgcaaggggg attattacct cggattatta tattcgcaag gttgttatcg gcgtttacaa
    22501 ttcgaacgga accgcagagt ttgattatac cggattgcct ggagaaaaaa gctatgatat
    22561 aacacatata aaatcgtagc ttcagacgac aacagctcca acgttgtcct tctcaacaag
    22621 agctttacgg ttacaagcac cggtcaggct gcgagcacat tgaaaataac caatgcaaaa
    22681 tatcccgata cccttgacgt gggagagggt ttttcggtaa cgggaattat ttcgtctaac
    22741 tataatataa cgcggattgt gataggtgca tataaagcgg acggcacaaa gggctt
//
LOCUS       DAWBSW010000008        21836 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_520_length_21836_cov_2.672283, whole genome shotgun sequence.
ACCESSION   DAWBSW010000008 DAWBSW010000000
VERSION     DAWBSW010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 21836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21836
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_520_length_21836_cov_2.672283"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2102..2111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2790..2799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3489..3498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5778..5787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11571..11593
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11910..11919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19229..19238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6325 a         4405 c         4841 g         6182 t
ORIGIN      
        1 caaaaaagga tttactgttt atcaccgcct ctttgcagta gggtgcatta tatttgagtt
       61 ctaattatgt tatgcccgtt tttcaagagc atcaaaattt atcaaatttt gcaaggtcaa
      121 ctcaaaaccg ctgattcaac cggcagtttt gattatgctt ttaaaaaatg cggtgcagag
      181 ttctcttaat agtaccgagc ttataatgca ataattgaca aacaattatt tcgagattat
      241 aattacaaat ataaaaaaag acctcactga agaggtcttt gggcacacaa tgtgcggttg
      301 aacgaattaa cgtcaacgat ttgagttaaa cttttatttg aactcacagt gtagttccat
      361 aggatcaaac tacacttata tgataacata ttgaaaggcg gttgtcaaga tgaaaagagg
      421 aaattattat ttgggtctcg atatcggtac agattcggta ggctacgcag ttacagacga
      481 aagctataat cttttgaaat ataatggcag tcctgcgtgg ggaacaacag tttttgatgc
      541 agtttcatca aatgccgaaa ggagatcgtt tcgttctgcg agaaggaggc ttgaccgacg
      601 ccagcaaaga gttaatctct tacaggattt atttgcaaga gaaattgcaa aggttgatga
      661 aaatttttat aagagaatta gagaaagtca actttacaga gaagatgtcg gagacaagta
      721 catattgttc aacgatacaa attatacaga tactgactat tttgccgagt atcccacaat
      781 tcatcatttg ataaatgagc ttgtgaaaaa tccggctccg catgatgtta ggcttgttta
      841 cctcgcttgt tcatggcttg ttgctcacag aggacatttc ctgaataaca tggaaaaagg
      901 caacatcagt gacgttaaag attttgagaa ggtatataaa aaattccttg agtattttac
      961 agagacaaga aaatgcgccg ccccgtggga atgtgatgat atagaagcgt tcggaaaagc
     1021 agttaaggca aagaaaaatg ttacggcaaa aagcaaggaa ctgattgagg tgcttcttaa
     1081 tgggcaaaag ccctcgaagg aaacaaccga ggagttcccg ttcaggcagg actgtataat
     1141 taaattgctc gcaggcggaa cctgtaagct gagtgatctg ttcgcttgtg aggagtatag
     1201 cgactacggg tcaatttctc tcggcatgga tgaagataca atgaaagaaa tctcgtcaaa
     1261 aatcggggat gattatgagc ttatctctgc aatgagagga atatatgatt ggtcactttt
     1321 ggttgatata ttgggcgaat acagcacaat ttcggaagca aaggctgctg tttatgagca
     1381 gcataaaaaa gatcttggaa cactgaaagc atttatcaaa gaatacaaac ccgagaaata
     1441 caatgaggtc ttcagagaaa tcggcaagga taattacgca gcatattctt accatacaga
     1501 tgagccgaat tcggcagggt taaaaagaaa aaatgttgag gacttttcaa aatacatact
     1561 tggaattatc aataaaattg agccgaagga ggaagacatt gaaaaattca acgatatgaa
     1621 atcccgactt gagcttcgca cgtttctgcc taagcagagg aatacgaata acagggtaat
     1681 tcctcatcag ctttattggc atgagcttaa tgaaatactc aagaacgcat cgaagtatct
     1741 tgatttccta aatgaaaaag atgaggacgg aataagcgtt aaagataaaa ttctgtcagt
     1801 ttttatgttt aaaataccgt acgcttaatg ataagtcacc gaatgcgtgg atcgtgcgta
     1861 aggcaggtag aatttatccg tggaattttg aaagcatggt tgatcttgat gcgagtgaag
     1921 atgcttttat caggaaaatg acaaatcgtt gtacatatct gcccgatgaa tatgtcttgc
     1981 cgaaggattc tttatgctat cacaagtttg cggttttgaa tgaaataaat aatttgaaaa
     2041 taaacggaga gaaaatttct gtcgagctga agcaaagaat atacaatgag ctgttcctcc
     2101 annnnnnnnn ncgtaaaagg attatggatt ttttgatttg caacggatat cttgaaaaag
     2161 gcagcgaaga tttggtaagc ggaatagata taaatatcaa atcaaatctt gagcctcaga
     2221 ttgcatttag aaggttattt gaaaacaata ttttgtcgga gtcagaggcg gaaagaatta
     2281 ttgaaagatc ctcatatgcc gaggataagc tgcgtttttt aaagtggttg aaacgggaat
     2341 ttccccaatt aaatgatgac gacagggaat atatcggcag attgaagttc aaagatttcg
     2401 gcagactgtc gagaaaattc ctatgtgact ttgagggagc ggacagaaaa accggagagg
     2461 tatttacaat tttagaggca ctttggagta cgcaaaataa cctgatggag ctcctttcaa
     2521 acagctttac ctttgccgaa agaatagaag aatatcaaaa agaatattac agcgaaaatc
     2581 cgaaaaccct taatgacagg ttaaacgaga tgtatatttc cgattcggtc aagcgtccta
     2641 tttacagaac acttgacata gtgaaggatg ttgtaaaagc gttcggtgtg ccgaagaaaa
     2701 tatttgttga aatgacacgc ggaggatctg ccgatcaaaa aggaaagaga acaaaatcaa
     2761 ggaagcagca aatacttgat ttatatgaan nnnnnnnnna tcataaacaa caaggtgctt
     2821 gtgttatagg agcttaacgg agaaaaggga gatatctatc cgatcaagtc gaatatcaga
     2881 caaaaaatgt tcggctggtg gaagcactta aaagataaag aactgataag cggcgaaaaa
     2941 tacaagagac ttacccgttc aactccgttc tcagacgatg aaaagcttgg ctttattaac
     3001 agacagctta ccgaaacatc acagtcaacc aaagccgttg ccacacttct cggagaaaaa
     3061 tacgataccg aaatcgtata ttgcaaggca aggcttacat ctgaattccg gcaggaattt
     3121 gacctgctta aatcgagaac ctttaatgat ttgcaccacg caaaggacgc atatctgaac
     3181 atcgtttgcg gaaatgttta taatatgaag tttactaaaa agtggttcaa tattgactcg
     3241 cagtacaaca taaaaacaaa ggcgctgttt tcgcatcctg ttatctgcgg agaaaaaacc
     3301 gtatgggacg gagagccgat gctcgcaaag gttaaggcga ctgtcgcgaa gaacaatgca
     3361 cattttacaa agtacaaatt cttcaaaaga ggtggattgt ttgatcaaaa tcctgtgcat
     3421 gccggggcgg ataaaactcc tctgaagaaa ggtttaccga ctgaaaaata cggcggatat
     3481 aataaggcnn nnnnnnnntt cttcattcct gtaaaatata cagccggcaa aaagacggaa
     3541 ataattataa tgtccgttga attgcttgtg ggaaagaagt tccttgcaga taaaaatttt
     3601 gctctggagt attcatattc aagacttcag cgtattctcg gaaaaccggt tgactcaata
     3661 tcattcccga tgggcatgac accgtggaag gtcaataccg tgctgtcact tgacggattc
     3721 agggtttgta ttaccggtat cggaagcggc ggcaagtgct taagtgctca gcctattatg
     3781 caattctccg atgataagtt ctgggaatac tatttgaaaa agctggaggc tgtctccgaa
     3841 aaaatagcac gcaaccccaa atatgtttac agtgaatatt ttgacaaagt ctctactgaa
     3901 aagaatatcg agctgtatga tttgtatttg aacaaactaa aatcaactat ttattcaaaa
     3961 agaataaata acccggttgc aatagttgaa tccggcaggg aaaaatttgt atctttaaat
     4021 ataagggatc aggtcttaac attgcttaat ttacatactg tgttcggaag aatgacgggg
     4081 ggctgtgatt tgagctgtat cggaggatct aagaaaactg ccgccacggt tagtttcagc
     4141 tcaactgttt ccaactggaa aaagtattac tcggatgttc gcattataga tcaatctgcc
     4201 tcaggcttgt gggaaaagaa atccgaaaat ctccttgatt tgttatgagt tggcggacgg
     4261 taataatttc caatcagtca aagcttgatt acaagatggg ctatcttgtt gtaagaggta
     4321 ttgaaacgcg cagggttcat attgatgaga tacttaacct tgttattgaa aatcctgccg
     4381 tttcaatgac cggctgcctt attgaagttt tggttgaaaa gaaaataaag gtaattttct
     4441 gtgattcaaa aaggaatccg acagccgagc ttgttcctca tcacggcagc catgactcgt
     4501 cggcaaaaat ccgcactcag gttgcgtggc aggacgatat caaagccgct ctatggaaag
     4561 aaataatcag cgagaagata cgaaaacagt ccgatttcct ttttgaattg aataaagaaa
     4621 gagaagcgga actgctttta tcgtatatcg gtcaggtcga aatgcttgat gcaactaacc
     4681 gagagggaca tgcggcaaaa gtgtatttta atgcattgtt cggtatggat tttacccgca
     4741 gtgccaatat tcccgtcaat gcagctctga attacggata cagcttgatt ctttcggctt
     4801 ttaaccgtga aattacggta aacggatacc tgactcagct cgggattttt cacaacaata
     4861 tgtttaatca ttttaatttg agctgcgatt taatggagcc tttcaggatc gcggttgacc
     4921 gagttgttta ttcggcaaag ccgacacagt ttgaaaaaga agaaaagcat gcaatgtgga
     4981 atatcctgga ccaaacagtt attatcgatt caacacatca aaacctgtcg agggcaatta
     5041 aaatctatac aagaagcgta tttgaagcaa tcaatgacgg agatgtttcg gaaattaaat
     5101 tttttgaatt tgaccttgat gatgacggat ttattattga catatgagtg gattcagatt
     5161 tatgagaata tttgtatttt ttgatttgcc gatggactct cttgaagaaa agagagaata
     5221 tcggaaattt agaaaagcgc tgataaaaaa cggatttatt atgatgcagg agtcggtgta
     5281 ttgcaagctt atgacgacac cctcggttga gaattccgtg aaaaatatga tccaaatgaa
     5341 taaaccgtct aagggagttg tgcaaacgct tgttgtaacc gaaaagcaat ttgttaagat
     5401 ggaatatgtt gtaggtgaga ataaaagcga tataatcgat aacgaggata gggtgattat
     5461 actttgaaat atgtttatcc cgagatcggc aaggtctttg aaaccggcag tgattttgta
     5521 aacacgcttg tcattgaaaa tcaaggtttg ttttgcagaa taatagagga ttttcaaaat
     5581 cagataaacg gctttgaagg aaaaggtgta cgctcgacgg atgataaaat tatgccgatg
     5641 aataaaaatc ttgaagtgtt ttcacaattt gtgccgtttg aaataaatcg aaaaaacttg
     5701 atatcaaaaa tttgttctgc cattgaagaa aaagcaataa acgaagaatt ttgttttaaa
     5761 acatcggaat tgatgacnnn nnnnnnncat cgttagttaa agcatcaggc ttggaaatag
     5821 cagacgatta tgactcgctc ggcgaaaaaa taattgattt gatggagttg attacggaat
     5881 ttgacagaga gaaattttat gttataataa atttacgcag ttatttatcc gatgaggaaa
     5941 cggaacgatt tatggatacg gtcatccggc atagattttc ggtgctgatg attgaaagca
     6001 acgagcattt attgttgccc cgggaaagcc gcttccttgt ggataaagac ttgtgtgaaa
     6061 ttagttgaca ttgctttgtt gtaccctttt gcattgctct cggcggttac aggccgcttt
     6121 cctcaaattc ggttttgagg tttgagagca gtgtaattcc atagggtact caaaccggga
     6181 atcagcatca agcgttattt cgtcagtttg agagcagtgt aattccatag ggtactcaaa
     6241 cagcgtcgtt tgactgcatg cggcatccgc agtttgagag cagtgtaatt ccatagggta
     6301 ctcaaactaa ctaattgacc gtcaacagtt gtggagtgtt tgagagcagt gtaattccat
     6361 agggtactca aaccggaaaa cacccacttc caatagaaca cttgtttgag agcagtgtaa
     6421 ttccataggg tactcaaact atattgttaa aggctctgac aaaattttag tttgagagca
     6481 gtgtaattcc atagggtact caaacacgct cgccgtcata agagttaaca acggcgtttg
     6541 agagcagtgt aattccatag ggtactcaaa caaaaactgc aataaaccgc caacaatacc
     6601 cgtttgagag cagtgtaatt ccatagggta ctcaaacaaa aagtattgac attttaaaaa
     6661 tgtgtaggtt tgagagcagt gtaattccat agggtactca aacgaaagta ttcttgagca
     6721 aatcaagcaa attgtttgag agcagtgtaa ttccataggg tactcaaact agtcgcccac
     6781 atatatgtcg aaatgtcttg tttgagagca gtgtaattcc atagggtact caaacttcaa
     6841 tgcagctttt gatatccgag ttattgtttg agagcagtgt aattccatag ggtactcaaa
     6901 ctgaggtata tggttataag cattctcgaa tgtttgagag cagtgtaatt ccatagggta
     6961 ctcaaacttt ttctcataat cgttaccgaa tttaaagttt gagagcagtg taattccata
     7021 gggtactcaa acattgttga gtaaaataaa taacatcaca gtgtttgaga gcagtgtaat
     7081 tccatagggt actcaaacat aaaaaaatct aatagaatat gggtatctgt ttgagagcag
     7141 tgtaattcca tagggtactc aaacaaaaag tattgacatt ttaaaaatgt gtaggtttga
     7201 gagcagtgta attccatagg gtactcaaac gaaagtattc ttgagcaaat caagcaaatt
     7261 gtttgagagc agtgtaattc catagggtac tcaaacagtg agcatatttg ttacagcata
     7321 ggaagcgttt gagagcagtg taattccata gggtactcaa acaattccag gggtatactt
     7381 atagaataca aggtttgaga gcagtgtaat tccatagggt actcaaacct attttgtaat
     7441 ttcctccggc gttgtagtgt ttgagagcag tgtaattcca tagggtactc aaacggttgc
     7501 aaaaacctga gaaaggcggt ataagtttga gagcagtgta attccatagg gtactcaaac
     7561 acactgtcac gccgagctgt cccataaggc gtttgagagc agtgtaattc catagggctt
     7621 tcttagcatt gtctgaaata aacataatta taaagcggag gactaaaatt gatgaacaag
     7681 tcctccgctt tataaaacta ttttttcacc agtttaaatt gaccttttgt caatagttgg
     7741 attagaaaac caaaaaaagt aatgaggcag tggcgatttt ttcgtcactg ccctgtttct
     7801 ttaaataaat attcaaaaga agcgttgcat atcaaataat attacaaaac cggagctttt
     7861 atctgatccg gctccgacat aaacagtccc gccgatacaa gcttatcgac atcgtaactg
     7921 tcaatttttc cgtcacggtt taggtctgca gcggcaagca ccgccctttg aggagtcaac
     7981 attccggatg cgatgagttg agcccaaaca gcgtctcgtc cgtcatgcag tccgtctccg
     8041 tcgaggtcgc cgaaaataac gacggtatat tctttaatgc ttttgtccgg atatgttacc
     8101 ttaacaacag tccccgtgcc gatatattct cccttcacgg tcagtgatat ttcagcgtaa
     8161 tcgcttgaaa agtattctcg gaatttatcg gaggtaatac cttgctttaa cccgtaaata
     8221 atcgaatcat cagcagagca attgtgggca aaccgcttga ttaccgtttt ctccgtttca
     8281 ccgcaaacgg tgcaggtgcg tatactttcg ccgtctgcgg ctgcagtcgg ctcttttgtg
     8341 accgtcggag cactccatat gtggtcattc ggattttcac aaccgataag tgccgtgtaa
     8401 gctttgattc ttccgacata ataaccgaga gtgtaccagt tatcaccgct cggaacgtaa
     8461 gaaagatcct tgcggtctcg gtatccggtg acgatttttg cgtcaccgtt attgtttgaa
     8521 accctgacaa taacagaaaa ccattcaccg ttttcaagcc tcacgggttc gttgagctca
     8581 actgtcacat atccgccgtg ttcaaaaaat ttcgttgttt tgccggcaga agctcctccc
     8641 gaaacgggta cattactctg tccgttgtac ggtgcgtcca aattcttgat tatttcggtt
     8701 tcaacggtta tgttctctcc ctgaacagca acattaactg cttttaaaaa ctcggacttt
     8761 ccgtttgttc ctcctttttt ggcttgatag acattagcta tacacttatc ttttctcaat
     8821 gaaaaatctt caaggttgcc gtcgtagcaa tagttgaaat catacttatc cttgggtgca
     8881 taggtgaaag cgtatgcgct cgagctggtg ttgtcatagg agagccagaa atattcgcat
     8941 gaactgtaac tgttctttac aagccatcct ccgttctgcg aagcgccgcc ggggatgaat
     9001 ttttcggcag gaatattgtc gttccagccg acaattgtgc aagcgtgggg tgaactggat
     9061 gaacccgact cgtttttcgg attatagtac tcgcattcac gcatattgtt atattgaaaa
     9121 gtgactgcac cgtactgtgc aatcgccttt ttaattgcga gaatcccttc tgcgggattt
     9181 gctttgttct ccggaatata gaaagcgttt tcaaatctgt aagtagggtt ttcaaacgga
     9241 tttatattcg cctgactgcc tgaaacgggt ccccaccaaa gagaaaaaat cttagcgatt
     9301 ttaagaggat tacctgttgc cttggtgtaa tcgacggact gccaatcgcc gttggtgttt
     9361 ccgagagggt cggattctct tctgtagatt ctgtaagctg ccgcaaccgg gttaaacgaa
     9421 aggctgtttt tgtcaacgtc ggggtcaatg ccgagacgca agatacttgt ttcggaagcc
     9481 gcaatcgaag aataagccca gcagagatta ctggtgccct gatccttaac gggagtaaca
     9541 attccgtatt ctctgccgtc atacacggac atttgcatta tttcttccgc agtcggaatt
     9601 gtcgcatgtt tcgccgaaat gctcggcata acgctgagca taagcaaaac ggttacaaca
     9661 atcaatgata taactctgtt tttcatcagg tgcatcactt cgctttcctt caattttttt
     9721 cattataccc taaaacaaac aaaaaaatca acccgtttga gcaatttgtg taaacgtttt
     9781 cttgacataa attcggctaa ttgatataat ataaaaaatt ttaaaatagg ttagttttga
     9841 tttcattaaa ggatttgata taaatgagaa aaacaaaaat tatttgcacg atcggccctg
     9901 catgccgcaa tgaggagact ttgactgaca tgatcagggc gggaatgaat gttgcaaggc
     9961 tcaatttttc gcacggaacg catgccgagc atcttgaaac aattgatatg ttgaaaagag
    10021 tcagagaaaa gctcaacgtg ccgcttgcaa ttatgcttga cacaaaaggt ccagagtacc
    10081 gtatcaggag ctttgaaaac ggttcggtat ttctcaatga cggtgatgat tttacgttta
    10141 caaccgacca aattgtcgga aatgagcata aggtttcggt tagctatccc ggccttgcgg
    10201 acgagctttt ggtcggcgat acaattcttc tcaacaacgc tttgctcaat tttgaggtcg
    10261 tctcgattga cggacatgac atcaactgcc gtgttgtttc gggcggtgag ctttccaaca
    10321 acaagagcat gagcttcccc ggaaagacac tcaagcagaa atacgtcagc gaacaggatc
    10381 gctccgatat acttttcggc attgaaaatg atgttgattt tattgcctgc tcctttgttt
    10441 cctgcaagca ggatcttctt gatatcaagg cagtcatggg agacagcgta aacagaattg
    10501 atcttatcgc gaagattgaa aaccgtacgg gaatcgacaa cattgatgat atctgcaccg
    10561 aatgtgacgg aattatgatt gcacgaggcg atatgggcgt tgaaattccg ttcgtggagc
    10621 tgccgataat tcagaaagcg cttatcacaa aatgccgact gctcggcaag cgtgttatca
    10681 cagccacaga gatgcttgaa tcaatgattc acaatccgag accgacgaga gccgagacct
    10741 cagacgttgc caacgctgtt tatgacggaa caagcgcgat catgctttcg ggcgagactg
    10801 cggcaggcaa atatcccgtt gaggcggttg acacaatggc acgaatcgca atgcagacct
    10861 agaaaaacat aaactataca aagcgcttcg gcacaaccga attcagaatc aagaatcctg
    10921 ttgatgcaat ttctcacgca acctgcggaa tggcaatcga tttgaacgca aaggttattg
    10981 ccgtttgctc cctttcggga atgacggtca gaatggtttc ccgtttccgc tcgcctgttg
    11041 atatcatggg aatgacggtc gacaaaaaga cgtggcgcaa gctttcaatg tcatggggtg
    11101 taactcccgt gctctgcgag acatacgaat caaccgacgt gcttttctat gcggcaaaga
    11161 acaaggcgat tgcgacattc ggacttgaat cgggtgacag aattattgtc acgggaggca
    11221 tgacgaacgg cgtttcgggt aacacgaatc ttatcaaggt tgaaactgta tgataaaaaa
    11281 taacggcatc ttccaaatcg gttgatgccg tttttgctat ttaactttta gcgttcgtaa
    11341 atatgatttc tgttcatctg taattctata gctttcgatt gctttctgaa tcgccttgtt
    11401 gtgtgtctgt ttttcgagac gcttgctttc gatataggga attatttcgt tatagttttt
    11461 tgcaagtgca gttgcaaagt accacgcaat catcatcttg acatagtatt cctcacgtgt
    11521 gactgcgcag acgcgttcgg cacattctgc tttaaaattc tcgccgagaa nnnnnnnnnn
    11581 nnnnnnnnnn nnntatcaga gccgagccag tcggggattt tcgagtaaag ctccggcaga
    11641 tgctttttga aaacgggaat tcgcatcata tcgcaggtcg cccagttgtc gacaaacgga
    11701 agaaatttgt caagcagttc tattgctttt tcgtagtctt tttcaaagcc gatcaaagcg
    11761 gcatggacgt tgttttcttc ataatacttg tgcggaagaa tttttaaata gctttcgtaa
    11821 tcgccgcttt tgtaaaactc ttttgcaaac ttttttaatt gcggcgagcg cactccgatg
    11881 acagtttctt tgtcaatcgt cggcatcagn nnnnnnnnnc gctgcatgaa aatcacggta
    11941 tttttcgtcc tgcaattcaa ataatttttc ttgaacatat ttttcaatct cattcatttt
    12001 tatcagacac tctctaaaaa atcttcaatt gctttcactc ttttttgaag cgaaaaatca
    12061 ttggcacgtt tcttttcctc gttactgaat ttttcgagaa gcgacggatt ttccaaaact
    12121 tttttcatcg cattgtaaag accgtcctcg gagttttcac agataattcc acattcgttt
    12181 tcgccgaaaa tttctctcat gcccgagcat tcggtcgtga taacaggcac gctcaatgca
    12241 accgcttcac tgactgaggt gctgtagccc tcggcatatg acgagcaaac atagacatcg
    12301 gagtgtgcaa tatatttata cggattcgtt tgaaaatcaa gagtttttat tgtatcaccg
    12361 agcttttttt cgacaattat ttttttaata tattcttttt cttcgccgtc acccattatg
    12421 agaaccgaaa aaatctttcc gtcatttttc agcctttcgc agacatttac aaagcgctca
    12481 aaacctttaa cctttacaaa gcttcctgca agaatgaact tcattccttt gccgaaatcg
    12541 aagtcgtttt tctctttgct cttttcaatt atgctgtcaa aatcaatcgg gttatagagc
    12601 acatgaacct tttttgccat gccgtatttt tgaatgaacg cctctttcat tgtttcggct
    12661 acgcagacga ttgcatcaaa attttcgtag cacttttttt cttcctctgc cgagccgaaa
    12721 attttctcgc tccacgggtt tactatcata tcggtatgaa cccatgcgat ttttttgcag
    12781 tttctttttt ttgagttacc gataattttt gttgaatatc cctcgcagaa ggcgacctca
    12841 acatcatagc ttcctttgat ataattcttc cttacaaatt cgggagaggc aagcagagac
    12901 gctttgattt ttaacttgtt aagaattgat tttgcgcctg agtccgattt ttttaccatg
    12961 cagcggtgat tggcgtgcga tttgatgata tccgtgtaaa cctcgccgtc ggtctcggtg
    13021 aaaacatcgt atttattttt tgtgttctca atgaacgttg caaaaattct ttccgcaccg
    13081 cctcgaccga gcgattcgat aaaaaacaaa acagatcgca taacgacatc tccgttgatt
    13141 tgatatttaa attatataaa atataaatgc ttatgtcaat gaaaaaagac tgcgccgagt
    13201 gacgcagtct ttgaaaatat gtgattattt gtacatcggg ccgtaaacct ggcatgcacg
    13261 gtagtcctga ccctctttgt ccataccgaa atagttccag tttgccggac ggaagatctt
    13321 ctcctcggga acgttatgaa gagaaacggg aatacgaagc attgaacaca ttgtgatgag
    13381 gtctgcaccg atatggccgt atgtagatgc gccgtggttt gcgccccagt tgttcatgag
    13441 gtcgtaagcc gacttgaaag cacccttgcc gttgcagatc ggagtgaacc atgtgcaggg
    13501 ccatgtgtag tctgttctct tccagagagc atctgttacg tcctcgggaa gtccgacata
    13561 ataaccctcg cagatctgaa ttgtcggtcc gataccctta acaaggttga tgcggatcat
    13621 tgttgcgggc atctcgttct tcgtaacgaa gcgtgaggaa tatccgccgc cacggaagta
    13681 accgttatcg gctgcgttcc atgttgtggc cttgagcatc ttgtcaacgt cctcgtcggt
    13741 tacgtcatac caaggcttca tgcaagcttc gcccttgtcg ttcttcagct cgccgcaagc
    13801 gtcaagacat gaggcgccgg agttgatgag gtggaggaat ccgcctgcct cttttgcgtg
    13861 accctcaagc tcatagcctg taactctctt aacggattcc gcactccagt gagtacgaac
    13921 gtcggagaac attgacggct ggtttgtgag gagcttctca aagagcatcg aaataccgtt
    13981 gagaacgtcg ttctctgttg caaggatgta aggctctctt acgccgtccc agtcaaagga
    14041 ggtgttgaga agtgcctcgg caaagtctgc gttcggccaa tggtctgtcc actgacgctg
    14101 accctggaag cctgcggcaa tagcattgtg acctgccatc tcttcctctc tgcccttggg
    14161 aagattcttg ttgccgttca tgagatcctt gatgatgcac atcatcttga ccgtgaactc
    14221 ccaatctttt tccttttgct cgtccgattt tcttacgaag tcggggttct tatcaaatcc
    14281 ctcggggcaa tgctcctttg tccacttgta agccttcttg tattcttcct cgtcatagat
    14341 gttttcttcc attcggcgga ggatttcaac ctcgtcaacg gactcaactc tcatgccgag
    14401 atattcctcg atgaaatcgg ggtcgattga agaacctgcg atacccatgc agatcgaacc
    14461 gatctgaaga tatgacttgc ccttcattgt cgcaactgca acagcggcac gtgtaaagcg
    14521 aagaagcttt tccttaacat cttctgtgat attctcggaa tcgtcagcct cctgaacctc
    14581 tctgccgtag atgccgaaag ccggaagacc cttctgagca tgagctgcaa gaacggatgc
    14641 aaggtaaact gcgcccggac gctctgttgc gttgaagccc caaacgccct tgatcgtgtt
    14701 aggatccatg tccattgtct cggaaccgta gcaccagcag ggagttacgg aaagagtaat
    14761 ggcaacgccc tctttcttga actgctcggc gcaggctgcc gtctcggcaa cacgaccgat
    14821 agttgtgtcg gagataacga ccttaaccgg ctcgccgttt gaatacttga ggttctcctc
    14881 aaagagcttc ttggctgcct tagccatgcc cattgtctga tcctcaagag actctctgac
    14941 cttaagaggt ccgcgtcttg cgtcgatgat aggacggata ccgattacgg ggtattcgcc
    15001 cagatatctg ttttctgcca tataaaattc atcctttcgt tgaattattt tttatataaa
    15061 tctgcaagca gatttttaac ttaatatgtt aatcaaccga agtgtaagtt tttgtgataa
    15121 tgccgttttc gtcatattca aggacatagg tcttatgcca taaaccggag acgccgacat
    15181 cgtatgttat tctattagac ccgtctgcgg atgtttcatc cgttcccttg ccgagcaagt
    15241 cgttaacctc ataagttttc attcctacaa ttttgtgctt ttcttcaaga tcgtcaatca
    15301 tgtattgacg gccttgcaca tgatttgaat agtttttcca ttttgcggag tcaaatgttt
    15361 tgtattctat gttgactact atagtgtttg ccaagaaaag aatgggtaca atgaaaacaa
    15421 ccagtgctgc ggatattatt acacagagct ttcgcttctt tttattgaac atcgcaaagc
    15481 acagggcagc aaaagcagcc gcaagcatta cggaaggtat taatttgccg ataaagctga
    15541 attttccgta gcttattgca tctcctgcca aaaacacaga gtctataaaa caaacagcga
    15601 taaagagtat aacgaaaatt gtcaaaagaa ttattttaat tgcagcattt gaatctttta
    15661 cctttttcat tttatcccct gcttgcatcg ttagttgttt caacttttgt aaccttaccg
    15721 ttttcgtcat aagtgattat gaatttttta tatcccggga attcctgtcc gatttcataa
    15781 tagaactcgt tatcttcggg agaatcatag ctgttttcgc cgagaatatt tttaacctct
    15841 ttaacgtcca tgccgacaag tccatacttg cttttgatat cgtcaatcat gtattgacgg
    15901 cagttataat attcggggtc gttccagatt tcctgattaa aggtctgata ctccttggca
    15961 gcaacaacgg tgttagctac gccgagagca ggaataacac ttattataag gataaaagaa
    16021 actattccgc tgacaagcct tttcttttta aagaaaattg taatgcaagt gcaaagatac
    16081 gccaaaacaa gaataacggc ggcaattatt tctccgagat gtccgtattt tcctgagctt
    16141 atcgcaggac cgataaagga cagcttcgct atataataaa ttccgcaaaa aattccagcg
    16201 aacaaaatcg ctaagagaat tttaagagtg attttcaatc cctttttctt atccattttt
    16261 gtttctccct ttaggcttac agccctgtat ttgcagtgag gcctgtaaaa taaatattgc
    16321 agattttata gcaggcgata aaagacaatc tgttgactac ccggcagtca ttgtcgagtt
    16381 caaagttgct ttaaattatt ttgagaaaat cctcataagc ccctgcccac ttgtccgtgt
    16441 ccttcggctc aaaggtttta agctcggaag agtcggcaac gatttttctt gcctgtccga
    16501 tatttttaac agaaccgtcg gacatgagct gaacggcaac gttgcccatg acggtagcct
    16561 cgatcggacc tgctttaacc gtgcggtcgc aggagtttgc cgtcatctga caaagcaagc
    16621 cgtcctttgt gccgccgccg ataacgtgaa taacggggta gtcacgctcc gtacattcgc
    16681 gaagcttttc aaatgtcagc ctgtatttca tcgcaagact ttcgtagata cagcgcatga
    16741 tctcgccgac cgtttcggga acgtactgac ctgttttgcg gcagaattca cgtacacgct
    16801 cgggaatatt tccgtgagga acaaactcgg gagtgtccgg gtcaatgaag catttgaacg
    16861 gctctgcggc aagcgcaagc ttttcaaggt cggcatagga atattccttg ccctcccgct
    16921 tccattggcg gcggctctcc tggatgagcc aaagtccgat tatattcttg agaaagcccg
    16981 tcgaaccgtc gaagccaccc tcgttcgtga tatttatgtt cattgaggtc tcattgacta
    17041 tcggtttgtc aagctctgtg ccgaagagcg accatgtgcc gctcgaaagg aaagcaaaat
    17101 cgccgtcctc gcacggaacg gaggtgatag ccgactgagt gtcgtgaccg cagacggaaa
    17161 taacgggaac aggatcaacg ccgcattcct cgcaaacttc ctttgaaagc tcgccgagaa
    17221 ccgtgcccgg ctgaacgagc ggagcaaaga cagattcttt aattccgaaa gcatcaagaa
    17281 tttcctttga ccacgttctt ttatcaaggt caatgagctg tgacgtggag gctatcgaat
    17341 attcggcaca cattttgccc gtgagcatat atccgaaaag gtcgggcata aagagcattt
    17401 tgtctgcacg ctcaagcatc cacggtctgt attttttaag tgaaataagc tgaaaaaggg
    17461 tattcagctc cataaactga attcccgtaa tgccgtaaag cttttctttc ggcatagtct
    17521 tgaatgcctc gtcaacaagt ccgacggttc ttgcgtcacg gtagtgaacg ggattttcca
    17581 tcagcttgcc ctcggagtcg ataagtccga aatcaacgcc ccatgtgtcg attccgattg
    17641 aatcaaagcc gcctgcgatt tttgctttga taattccctg cttgatctca taaaaaaggc
    17701 gcagaacatc ccagtacacc gtgccgccga cacttacagg gtcgtttgag aaacggtgca
    17761 cttcttcaag tgtgatttta tcgccgtcga agcagccgat tattgctctg ccgcttgagg
    17821 cgccgaagtc aaaggctaaa actcttttag tcatttatta caacaccctt ttggagaata
    17881 atattagcgt aaattgcagt ctcacccgtt gcaacgattg caaaagcgtt ctttgcctta
    17941 tcgtagaaat caaaacgctc aataagtccg atattcttat cgccctcgtt tttctcaacg
    18001 atctctttgt atgtagccca gatctcggga cggttgtcgg aatcgccgtt atccatgagg
    18061 gaaacgggag cgtcaacata tgtgtcaagc ggcatgagct tgagtatagc gtcgagaatt
    18121 tcgggaacgc cgtgaccgtc aaggcgaaca agacgctgag catttgtggc tgccgggaaa
    18181 ttgccgtcgc cgattacgat gtagtcgccg tgacccatct ccatgaggat tttaagaagc
    18241 tcgggtgaaa gtatcgaagg tatgttttta agcataataa tatactcctt ttatctatat
    18301 ttagtttatt ataactgatg tttaaagaaa aataaagaga aatacaggaa aaagttacat
    18361 aaattcggtt gtaatttttt gtttgagcta ttaaaatgtt agataagggc aattatctaa
    18421 tgtattagtc tatattcaag cagtaaaagt ataatattat ctcacaagaa agtatattac
    18481 ggagagatat cttatgataa agttagatgt tttgaattta cttgaaaaga acggcaaaac
    18541 aaaatactgg ctctataagc agctcggaat gagctatcag aatttcagcc gcatgataaa
    18601 caacgaaacc aagtcgattc agtatgaaaa tatcgacgca ctctgtcaga tttttaactg
    18661 cacgccgaat gagcttttta aaatgacgga tgagtaaaga aaaaacggaa gattactcga
    18721 ctatcagtct ccccttattg taaggggagg gggaccgctt gcggtggtgg ggataaaatc
    18781 acaagccgca atcgaaaaaa tctaacgaaa gaggatgaaa gaatgtttgc aaaggtcaac
    18841 agtatgggca ttttcggcat ggaggttttc cctgtcagcg ttgaggctga tatatcctcg
    18901 ggcttgcctc gttttgatgt ggtaggcctg cccgattccg ctgtcagcga gtccagaaac
    18961 cgtgttcatt ccgcgataaa gaacagcggc tttgactttc ctgtcagccg aattaccgtt
    19021 aatcttgccc ctgccgaccg ccgaaaggag ggtcctctct atgaccttgc aatgtttgtt
    19081 gctttgctta tggcgtcgga acaactgaga gccgacacgg acggatatat cttcctcggc
    19141 gagctttcgc ttgacggaaa ggtgcgcagg gtcaacggcg ttttgccgat ggttatcgga
    19201 gcttgccaga gcggagtcag taaaatttnn nnnnnnnnaa gccgatgaga tccgaaggct
    19261 cggaagcctt tgactatatg cctgatttcg ccgatgtcaa gggtcagttt gaagctaagc
    19321 gtgctttgga ggttgcagcg gcaggcggtc ataatacgct gatgataggc agcccgggct
    19381 cgggcaaaag tatgcttgcc aagagaatcc cgtcgattct gccgtcgatg acctttgagg
    19441 aaagccttga gacaacaaag atttattcga tatcaggtgc gctgaagaac aattcgagcc
    19501 ttataactca gcggcctttc cgtgctccgc accataccgt ttcgtcaatc ggacttgccg
    19561 gcggcggagc gatcccgaaa cccggtgaaa tctcgcttgc tcacaacggt gttctgtttc
    19621 ttgacgagtt gcccgaattt tcacgtcagg caatggagat gatgcggcag ccgcttgaag
    19681 acggaaaaat cacaatttca agagcgtcgg gaacctcaac atatccttgc tcgattatgc
    19741 tcgtttgtgc aatgaacccc tgcccatgcg gctattacgg acacccgaca agagaatgta
    19801 cctgccgcga cggtgcggcg gcaaagtatc tttcaaagat aagcggtccg ttgcttgacc
    19861 gaattgatat acatatcgag gtgccgcccg tggactttga aaagcttgcc gacgagcacc
    19921 gtggagagcc gtcctcggag attaaaaaga gagttgaagc ggcgagaagg attcaacaaa
    19981 accgcctgag cggaacctcg gcaaactgca atgcacaaat gaacgctgca atgacccgtg
    20041 aattctgcaa ggcaagcgat gcggcaatga agatgcttca ggttgccttt gacagactcg
    20101 gactttccgc cagagcatac gataaaattc taagggtcgc aaggacggtt gcggaccttg
    20161 acaattcgcc gacaatcgaa gcgtcgcaca ttgccgaagc gattcaatat cgctctctgg
    20221 acagaaaatt ttggacaagg taagcaaatc gtaaaaatgt gaattattga cacaaaataa
    20281 aggtatgata taattaaaat agcttattat atcgtacctt ttaaattatt tgagaaggga
    20341 attcaattta tgaaagaaaa taaaaagaat ataccccgaa aaacaatata ttatgagaac
    20401 gaattgaccg acgattttgc aaaaatgggc attgaaataa aacctctgcg tgaaaactac
    20461 cgttatattc acagaggtcc gttgtggcgt cttttctcat tcattcttta cagaatcata
    20521 ggtcagccgc ttgcgtggct ttatactaaa attgtgccgc ttcaccgttt taagaataaa
    20581 aaggctattc tgaaagtggg catttccggc gcatatgttt acgcaaatca caccaatttc
    20641 attcttgatg cctttttacc gaatttgatc agacatttcc gcaggggata tatcgttgtc
    20701 ggtcccgata cgatgtcggt tccgggactt aatactatag ttgaaatgct cggcgcaatt
    20761 ccgctcggct caacgatcaa acagaaaaaa gaaatgataa ggtgcgtcaa aacacgcata
    20821 cgtcagagaa accttgtaac gatttatccc gaggcgcaca tgtggccgta ctatacagga
    20881 ataaggcctt ttacggacgc atccttcagt taccctgctc atacgggcac acctgttttt
    20941 gcgatgacaa cctgctatca gaaaagaaaa atcggcagct tcccaaaaac agtaacattt
    21001 attgacgggc cgttttatcc cgatgaaagc ctgccgatgg ctgagagaaa aaaggttctg
    21061 agagatcagt gttataacgc aatgaaagac cgcgccgaaa agaattcgac ctatgagtac
    21121 attgcttaca ggaagaaaga ggacggcgaa agcaaagact gattttgccg tacaaaatga
    21181 ataatatgta aaatttttca tagatattga ttagtaaaat atttatgtta taataggggt
    21241 aagcgacagg aatatgtcgt aaaaggagga aaagaacaat gacaagacaa cagatgaagg
    21301 caatggcaaa agcacagctc gggaatcaga ttttcggcaa tacgtggctt atcgccgttg
    21361 gcgtgattac gattcagacg gctatttcat attgttaacg caattcccgt agccggtcag
    21421 gttgcatcac ttttggttat gggaccgatt gcatacggtg ttgcatcaat gtttctcaag
    21481 cagacccgca cgggcgaaaa aatggatatc ggtgatattt ttaacggctt caaagacgat
    21541 ttttcgaaca cgttccttat cggacttatg acgatgattt ttaccgtttt gtggagcctg
    21601 ctttttatca ttccgggtat cattaagtca tacagctatt caatggcggt gtatatcaag
    21661 gttgatcatc ccgactatga ttggaagcag tgtattgatg aaagtcagag aatgatgcag
    21721 ggacacaagt gggagctttt tgtgcttgac ctcagcttta tcggttggct ttttgtcggc
    21781 gcatgctgct gcggagtcgg tacactctgg gtaaatgctt atatggctgc tgcgca
//
LOCUS       DAWBSW010000009        20530 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_569_length_20530_cov_3.014505, whole genome shotgun sequence.
ACCESSION   DAWBSW010000009 DAWBSW010000000
VERSION     DAWBSW010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 20530)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20530)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20530
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_569_length_20530_cov_3.014505"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2961..2970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8760..8769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10829..10838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11484..11493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5302 a         4973 c         4645 g         5570 t
ORIGIN      
        1 gtataggatt ataatccaat tataatacat tcattcacgt ttgtaaataa gtttagcaca
       61 atttttgttt ttttattaaa taaattttct ttttgaggat aatagcagtg aaataataaa
      121 tctttattgc ttttgattga atcgataaat ccatatatat ttatatttaa aattcaaaac
      181 ctattgaatt attcttttat ttctctctaa tgatgccgtc ttgttatgca cgtcacgtcc
      241 tcaaacagct ttttcatttc aaaagcaaat tgcataactc cccaaatcgt cattatgcat
      301 aaaattaagt tttttaatat atgcaaaatg cactgtgatt tttgaaagca ataacattat
      361 ttatataaat atttattatg tctttacttt tcttgaaaaa tttgttaaaa tactattata
      421 tctttcgtga ggtgatatgc atgtccgaaa aattaggtgt agccattata ggctgcggca
      481 acatttcccg ttcacacata ccgagctatc ttaaacttga caatgttgag atacgctatt
      541 tttgtgatat tattcccgag cgcgccgaca atgctgttga gacctacggc tgcggcaagg
      601 ctgtgtataa ttacaaggag cttctcggcc acgacgatat agacattgtt tccgtttgca
      661 cccacaacga ctgtcatgca ccgatttcga tcgactttat gcgtgacggc aaagacgtgc
      721 tttgcgaaaa gcctgccgca agaatccttt ccgaggctct tgaaatgcaa aaggttgctc
      781 acgagacggg cagaatgctt tcaatcggtg ttgtaaaccg atttgtcgat acagttaata
      841 aaatcaaaga ccttatcgct gacggtgttc tcggcgaggt ttatcaggtg tatatcagct
      901 tcaggtcgca ccgctcgatc ccgggtctcg gaggcgattt taccacgaag gcagcttccg
      961 gcggcggagt tctcattgat tggggcgttc actttattga ccttgttctt tattgtcttg
     1021 atgatccgac tcccctcacc tgttcgggtg tcgctcattc aaagctcggc acagcgatga
     1081 aagattatgt ctacacaagt atgtgggctg aggacaccaa ggatacggaa aacggcacat
     1141 tcgacgttga cgattttgtt acgggactca tcagaaccga caaagcggca attaccttca
     1201 acggcgcatg ggcacagaac ataaacgaga atgaattgtt tgtcgatttt ctcggcacaa
     1261 agagcggcat aagaatgcaa tacggcggaa aattcaagct ctattcaaca atgaacgata
     1321 tgcttacgga tatcacaccc gactacaact caacaggctt tgactatgcg gcagaaatca
     1381 aatctttcgt agactccgtt atcacaagaa agccaaatca agcatatatt gacaaagccg
     1441 taataacatc aaaaattctt gacgctattt acagatcctc ggattccggt aaagaggtca
     1501 cattataata aaaggaaggc aaaacaatgg atattaaaat tttcgacaac gcagagcaga
     1561 ttgcttgtgc ggcagctcag ctttacaagg atcttatagg aaacaagccg aatgcagttc
     1621 tcggactcgc aacgggcgca actcccgtac ctacatataa gaagcttatt gagctttaca
     1681 ataacggcga aatcagcttc aaggacgtta caacattcaa tcttgatgaa tattgtgact
     1741 tggacaagta tgacaagaac agctactaca ccttcatgca cgagaatctt ttcaatcatg
     1801 ttgatgtcaa agaggagaat gttaacttcc ttaacggcaa tgctaaggat tgcgatgcag
     1861 agagcaagcg ctatgccgaa gcaattaagg ctgcaggcgg aattgacctc cagcttctcg
     1921 gaatcggtac aaacggtcac atcgctttca atgaacctgc cgacaagttc acagacgagg
     1981 ctttcaaggt tacgctcact cagagcacca taaattcaaa tcagaaatac ttcggcgacg
     2041 ttccgatgcc gagatacgca atgacaatgg gcatcggctc aatcatgcgc tcgaagaaga
     2101 ttcttctcat cgcaacgggc gaaagcaaag cgaaggcaat caaagccatg gtttcgggtg
     2161 aaattacacc gcagattccc tgctcaatcc tcaatgagca taacgatgtt gttgttatgc
     2221 ttgataaggc ggcagcaagt ctgcttgact gataaatata acaaatttac ggcagtatcc
     2281 tcacgggtac tgccgttttc attttgacac agcttcgcac aaaatgtcag aaaaaatcct
     2341 ctcctttgaa cagggagagg atttgtttat ttaattatac gcccttgaga agctcaccga
     2401 gtactgttgt tgagtgatcc tcggtcggtc tgagttcaac ctcggaatag cacttcatgc
     2461 cggtacctga cgggagaagc ttaccgatga taacattctc tttaagtccg agaagaggat
     2521 cggtcttgcc cttaatcgca gcgtctgtaa gaactctcgt tgtctcctgg aaggaagcag
     2581 ccgaaaggaa cgactccgtt gcaagagatg cctttgtgat acccataaga gtcggtacgc
     2641 aggttgcgta gttaggctct ttaccctcgg cctcggcagc agccttaacc ttttcgttgg
     2701 caacatagaa ttcatgcttc tcaacgattg aaccgggcag gaagtctgta tcgccggcat
     2761 cctcaatctt aaccttacgc atcatctgac gaacgataac ctcgatatgc ttatcgttga
     2821 tatcaacacc ctgtgtacgg tatgtcttct gaacttcacg gataaggtaa tcatgaacag
     2881 ccgaaacgcc tctgattgca agaacgtcat gcggattcgg agcaccgtcg gtaagggcct
     2941 gaccctttgt aacacggtcg nnnnnnnnnn taacgatctc acggaagccg taaggaataa
     3001 ggtaggattt cgactcgccg ttctcgctgt ctgtgataac aacgtggcgg ctcttcttaa
     3061 cgtcctcgaa tgaaactgta ccggatatct ccgaaagaat tgcaagagcc ttcggctttc
     3121 ttgcctcgaa cagttcttcg atacgcggaa gaccctgcgt gatatcgccg gcatccttgg
     3181 caccgccggt gtggaatgtt ctcattgtaa gctgtgtacc gggctcaccg attgactgtg
     3241 cggcgatgat accgacagct tcaccgatcg taaccggact acctgttgca aggtttgaac
     3301 cgtagcactt gatacatacg ccgtgctcgg tcttacaggt gagaagcgaa cggataacaa
     3361 cggaagctac cgagccttcc tctgcttcgc ctgcatttat ctttgcctga tgatttgcct
     3421 ttgcgtgagt tgcaactctc tctgcatcct cggctgtcat cattctggag ttctccatga
     3481 tgacctcgcc tgtctcctca tcaacataat cgttaagaag gaaacggcct gtgagacgtt
     3541 ctgcaaggtc aacgatctta cggttgccgt ggaagatgtc ataaacctca acgccctctg
     3601 tgcagtggca gtcctcctca cggataatgc actcctgaga aacatcaaca agacgacggg
     3661 taaggtaacc cgagtcggct gtacgaagcg ctgtatcggc aagtccttta cgagcaccac
     3721 gggatgaaat gaagtattca agtatgttaa gaccctcacg gtagttagcc ttgatgggaa
     3781 cttcgattgt cttacctgct gtgttggcga taagtccacg catacctgcg agctgacgaa
     3841 gctgtgaatc gttaccacgg gcaccggaat caagcatcat gttgatcggg ttgtatttat
     3901 cgaagtttgc cttgagttcc ttggcaacct cctgagtaca attctcccaa acctcgataa
     3961 ctcggtcgga acgctcggag tttgtgataa gacctcgctc gtagttacgg ttgatcttat
     4021 caaccttagc ctcggcatct gcgataagct ctgccttctt cggcggaatt gttgcgtcaa
     4081 acgcagcaac ggtgataccg cttcgtgttg agtgcttata gccctgagcc ttaatattat
     4141 caagcatttc cgccgcaacg gctgtgccgt ggacacggat acacttgtcg atgatcttgc
     4201 cgagattgcc cttcttaacg aggaagtcga tctcaagctt gaacatgctg tctatatctt
     4261 cggggttacg ctcaacaaag ccgagatcct gcggaatagg attgttgaag atgaccttac
     4321 cgagagttgt cggaacaagg cgtgtatact caacgccgtt gatcgttttc ttcatgcgaa
     4381 tcttgacaga tgaatggaga gtgatgatac cctcatcata tgccataata gcttcctcaa
     4441 cgttgcggaa agccttaccc tcgcccggct cacccggctt ctcaagagtc aggtaatagg
     4501 aaccgaggat catatcctga gtcggaacgg caacaggacg tccgtccgac ggcttgagga
     4561 ggttgttagc ggcaagcatg aggaatcttg cttcagcctg tgcctcagca gaaagaggaa
     4621 cgtgaacagc catctggtca ccgtcgaagt cagcgttgaa agctgtacaa acgagcggat
     4681 gaagcttaat ggcacgaccc tcaacaagta ccggctcaaa tgcctgaata ccgagtctgt
     4741 gaagtgtcgg tgcacggttg agcattaccg gatgatcctt gataacaact tcaagtgcat
     4801 cccaaacctc cggatgtacc ttttcaacca tctttcttgc agacttaacg ttgcctgcct
     4861 tttctgtttc aacaaggcgc ttcataacga agggcttgaa aagctcaagt gccatttcct
     4921 tcgggagacc gcactgatag agcttaagct caggaccgac aacgataacc gaacggcctg
     4981 aatagtcaac acgcttaccg agaaggttct gacggaagcg tccctgctta cccttgagca
     5041 tatcggaaag agacttgagt gctctgttgt tcggacccgt taccggacgg cctcttctgc
     5101 cattatcaat aagggcgtca acggcctcct gcaacattct cttttcgttg cgcacgatga
     5161 tgtcaggtgc gccaagctca agaagcttct tgagacggtt attacggtta ataacacgac
     5221 ggtaaagatc attaagatcc gatgttgcaa atctgccgcc gtcgagctga accatgggac
     5281 ggatatccgg cggaataacc ggaacaacat cgagaatcat ccactcgggc ttgttgccgg
     5341 atgctctgaa agcctcgatc acctcaagac gtttgagagc ctttgctctc ttctgagccg
     5401 atgtgccgtt aagctcctca cgaagctcaa ccgaaagagc gtcaagatca acctcctgga
     5461 gaagggactt gatagcctcg gcacccatgc ctgccttgaa atcgtcctcg tatctttcac
     5521 gatactccat atatgccttc tcgtcaagga tctgcttctt ctcaagcgga gtatctcccg
     5581 gatcggttac gatatacgat gcgaaataga gaaccttttc aaggtttctc ggtgtaatgt
     5641 caagaacaag acctattctt gacggaattc ccttgaaata ccagatatgg gaaacaggag
     5701 cagcaagctc aatgtgaccc atacgttcac ggcgaacctt ggcttttgta acttcaacgc
     5761 cgcagcggtc acagatgatg cccttgtggc gaactctttt atactttccg cagtggcact
     5821 cccagtcctt ggtgggtccg aaaattcttt cgcagaaaag tccgtctttt tccggcttaa
     5881 gggtacggta atttatagtt tcgggcttgg ttacctcacc gtaagaccat tctctgatct
     5941 gttccggtga agcaagaccg attttaattg attcaaaggt attgttttcc aatctgaatg
     6001 ccccttttcc tatggcaaag cctcaaaatg cggcattgcc tctaatctat ttcaatacaa
     6061 cttgcagtct gttatcaatc gaagctttcc tcgtcgtcat cggagaacat atcgtcaatg
     6121 tcctcgaaga tttcgtcaaa ggattcttcg tcgtcctcat cctcaatgag gttgccttct
     6181 tcgtcctcaa gagcatagcc ctctgcgccc tctgcatcat tgacctgtgt aaacgcttta
     6241 tcatccgaag aagtaaatcc aagctcatcg tcaaaattct gcttaagatc tatctcgttt
     6301 tcgtccttgt cataaacacg aacatcaaga ccgagtgact gaagctcctt aacaagaacc
     6361 ttgaaggatt cgggaatacc cgactgagga acattgttac ccttaacgat cgcctcgtat
     6421 gtctttacac gtcctacaac gtcatccgat ttgactgtaa ggatttcctg aagtgtgtat
     6481 gctgcgccgt aagcttcgag cgcccaaact tccatctcac cgaaacgctg accgccgaac
     6541 tgagctttac cgcccaaagg ctgctgagta accagtgaat acggtcccgt tgaacgggca
     6601 tggattttat cgtcaacaag gtgcaacagc ttgaggtaat acatgatacc gactgttacg
     6661 gggttatcaa acttgcgtcc cgtgcgtccg tctgtaagga ttgacttgcc gtcctcgcga
     6721 ataccgagat ccttaaacat ttccttgata tccgactcat gtgcaccgtc gaatacggga
     6781 gtcatgactc tccagccgag atgcttagcg gcaaatccga ggttaacctc aagaacctga
     6841 ccgatattca ttcgggaagg aacgcccaac gggttaagaa cgatgtcaag cggtgtaccg
     6901 tcctcaagga acggcatatc ctcctgcgga agaatacggg aaacaacgcc cttatttccg
     6961 tgacggcctg ccatcttatc accgacggaa agcttacgct tctgagcgat gtagcagcga
     7021 acaaccttgt ttacgccggg gctgagctcg tcactgtttt ctcttgtgaa tacttcaaca
     7081 ttaacgataa taccgtactc gccgtgcggt acacgaagtg aagtatctct gacttcctta
     7141 gccttctcgc cgaagatagc acggagaagt ctctcctcgg gagtaagctc cgtttcgccc
     7201 ttcggagtta ccttgccgac aaggatatcg cctgagcgaa cctctgcgcc gacacggata
     7261 attccgcgct cgtcaaggtc tttaagagcg tcatcaccga cgttcgggat atctcttgtg
     7321 atctcttcgg gtccgagctt ggtgtctctt gcttcaattt caaattcttc tatatgaatc
     7381 gatgtgtaaa catcatctct tacgagcttt tcgttaataa gaacggcatc ctcgtagttg
     7441 tagccttccc aggtcatgaa gccgataaga gcattcttgc cgagtgaaat ctcaccgtga
     7501 tcggttgccg gaccgtcggc aataacgtcg cctgccttaa cctgctggcc gagatcaacg
     7561 atcggcttct ggttaataca tgtaccctgg ttggagcgca tgaactttgt gagattgtat
     7621 ctgtcgatac cgtctgcagt ctgaatttca acataatcgg cattgacgtg aactacgaca
     7681 ccgtccttct tgcaaagaac aactgtaccg gagtcaaccg ctgccttata ctccataccg
     7741 gttccgacga tcggagcctc tgtgcggaga agcggaactg cctgtcgctg catgtttgaa
     7801 cccatgaggg cacggtttgc gtcatcgttc tcaaggaacg gaatcattgc tgttgcaacc
     7861 gaaacgagca tcttcggcga aacgtccatg aagctgactt ttgaattgtc aatctcgtgg
     7921 aattcgtcac ggtaacgagc catgacctta cgtctcatga aacggccatc ctcgtcgagc
     7981 ggctcgttag cctgagcaac gatgaactcg tcctcctgat ccgctgtcat gtaaacaacc
     8041 tcgtcgagga ccttgccgtc aacaacccgt ctgtacggag cctcgataaa gccgtatttg
     8101 tttatctttg caaaggttgc aagataggag ataagtccga tgttgggacc ttcgggagtc
     8161 tcgatgggac acatacggcc gtaatggctg tagtgaacat ctcgaacctc gaatcctgca
     8221 cggtcacggg aaagtcctcc ggggccgagt gccgaaagac ggcgcttatg agtaagctcg
     8281 gcaagcgggt ttgtctgatc catgaactgt gaaagcggtg atgagccgaa gaattccttg
     8341 atcgccataa gcacggggcg aatgttgata agagtctgcg gcgttatttt gtcgtcgtcc
     8401 tcctgagcct gaagggtcat tctttcacga acaactcttt ccattcttgc aagaccgatt
     8461 ctgaactggt tctgaagaag ctcgcctacg gaacggatac gacggttacc aaggtggtcg
     8521 atatcgtccg ttttgccgat gccgaaagca atggagttaa cgtagttgat tgatgaaaag
     8581 atatcatcaa ttgtaatatg cttaggaatg aggtcatcac aacgctcttt gagaagtccg
     8641 agaagtgcgt tatcatcctc aacgtcagcc tcaagaattt ccttaagagc cgagaaagaa
     8701 accttctcat tgatgccgat ctcgtcaagc tgcttctccg tgaattgagg gaagtagggn
     8761 nnnnnnnnnt gagataacct tgatttcctt atcgtcgtca accttaacga atacaacgtt
     8821 gacgcctgcc tgctcgatct cagtagcctt ctccttggag actacctcac cctcctctgc
     8881 gagaagctcg ccggtgagcg gtgcgataac aggctgtgaa agtgttgcgc ctacaatacg
     8941 gtcaaacaaa gaaagcttct tgttgtattt gtatctgccg actctggaaa tatcgtaacg
     9001 gtacggatca aagaacagat tgtcaagatg agtctgtgcg gtttcgagag ttgcaggctc
     9061 gccgggacga agcttcttga atacttccat taaagcctct tcggtgttct gcgtctcgtc
     9121 tctttcaagc gtagcctcaa gtcgaggatc gtatccgaag aattcacgga tatcagtatt
     9181 tgagctgaga cctaaagctc tgataaaggt tgtgataaag atctttctgt tcttatcaat
     9241 acgaacatag aaaagatcgt ttacatcggt ctcatactca agccaagctc ctcggttcgg
     9301 gataacggtg tttgagtaaa gctcaatacc cgtcttatca tgggtcatat cgtaatatac
     9361 gcccggtgaa cgaacaagct gcgatactat cgcacgctcg gcaccgttga tgataaaggt
     9421 accgctgtcc gtcattatcg ggaaatcacc catatagact tcgctctctt ttacgacgcc
     9481 ggtttccttg ttgagaagac gtgcctttac gcgcatcggt gcagaataac ttgcatcacg
     9541 ctccttgcac tctctgattg aatacttggg cttgtcgtcc attctgtaat cgacaaactt
     9601 gagtacccag ttaccggtat aatcggtaat atccgcgata tcacggaaag cctctttaag
     9661 tccggtctcc agaaaccacc tgtatgagtc cttctgaacc tcaatgaggt ttggcatttc
     9721 cataacctct ttgatttttc cgaagctcat tctggtcctg ctgccccttg caacgggaga
     9781 aacattcgtc attgactaac cactccgttc tttttatttt cgtccgtgtg tgaaacttat
     9841 acttatgaat tacttgtgaa atatgacagt ttttgtcatg aaaacgggca ttatttttcc
     9901 aaggaaaaaa gacccaagga cacacaatgc cattataatc gttatagcat tatacttcat
     9961 tagttaaaat atgtcaagca aaaaagcaaa aaaaataaag atattttgaa aaattttcaa
    10021 aaagtgattt ttcatagtta attccgtatt aacggtcgat ataaatcgct gtaaagaata
    10081 aaaaatctat tctcacccct cccctcttat ttcaaagata cataatttga atataaaagc
    10141 aaaaacgata aaagtaacgg cacaggaaaa cctgcgccgt cgaaattatt attttattgt
    10201 accgtacaat tgactgttgc tttagatatt aaagaccgta ttctgccgca acgtctgcaa
    10261 tcttaaccgg catacctgtc tcaagagact tctttgcggc aatgccgata agtacgggct
    10321 ggaggccgca ttcaaggccg acagtgatcg gcttgtcgtt aacaacacag tcaacgaact
    10381 gagaaacctc ctcaacgtaa gctcccatgt atctctcaag gaagaagtta agcggaacct
    10441 caccgtgaac gccgttagcg tcggaaacaa ctgctgttga ctcagagtca tttgaaatag
    10501 caacctgacc gagtgagccg aaagcctcaa ctctctggtc gtagccgtag cttgcacggc
    10561 ggcagttatc aataactgcg attgcgccgt tagcgagctt catggagata acggccgtgt
    10621 cgatgtcgcc tgcctcaccg attgccggat caacaagaac cgcgccgtat gcgaaaactt
    10681 cctcaacctc tgaacctgac atgaagcgaa ccatatcgaa atcatgaata gtcatatcaa
    10741 ggaagatacc gcccgaaacc ttgatgtagc ttaccggcgg tgcgcccggg tcacgggagc
    10801 aaaccttgag gatattaagg tcgcctatnn nnnnnnnngc cctcaacagc ctttcttgcg
    10861 gccttgaagt tgtggtcaaa gcggcggtta aagcctacct gatacttaac gttgctgttt
    10921 tcaagagcct tcttaacctc aaggatcttg tttacgtcat ggtcgatcgg cttctcgcag
    10981 aagatgtgct tgcctgcggc aatagcttcg agagaaatcg ttgaatgtgt gtcggttgat
    11041 gagcagataa gaacagcctg aatgtcctta tcctcaagaa tcttatgata atcggtgtag
    11101 aactcattta caccgagtcc ctttgcccac tcttttgttt cgtcattgag gaacggatcg
    11161 gcaatagcct taaccgtagc tgacttaaca tatctcatga ttgactcgca gtgtacctta
    11221 ccgatacggc ctgcgccgat aataccaaca tttaacatag ctttatgccc tcctgattat
    11281 attgtgccgt cccatgtgga aacttctgtt ttcttcattt cttcctcgtc gaaccaacgt
    11341 gttgttacgc acttgctctc tgtgaagaag cgatatgcgt ccttgccgag gcagtggaga
    11401 tcgccgaaga acgaatcctt atgacctgcg aacgggaaga atccgatcgg aacgggaata
    11461 ccaacgttta cgccgaccat gccnnnnnnn nnntctgagt gaagataacc gagccgtttg
    11521 catacgggtt agcgttcatg actgccagac cctcttcaaa gctcttaact ctcttgatgc
    11581 aaagaacggg gccgaatacc tcgtcacggc cgattgtcat gtcctctgta acatgatcaa
    11641 agattgtcgg gcctacatag aaaccgtttt catagccctc aacaacgcag tttctgccgt
    11701 caagcacaag ctctgcgccc tctgcaacgc ccttgtcgat gtctgcaaga acttccttgt
    11761 agtgcttctc atatgtaatt ggaccaagct ttgaggtctt atcgtatgca gggccgagtt
    11821 tgagtccctt agcctgctta acaagctctg caacaaactt atctgcgatg ctctcttcga
    11881 caacgcagca gctcagagcc atgcatctct gaccggcaca accgaatgcg gagttcataa
    11941 cacctgccgc ggttctctcg atcggagtat ctgcaagaac aagagcgtgg ttcttagcct
    12001 ggcaaaggca ctggactctc ttgcctgcgc ttgcagcctt ttcatatata agcttgccga
    12061 cgggagtgga gccgacaaat gtgataccct tgacatcagg gctttcaaga atcgtgttga
    12121 tctcgtttct cgaacaggtg acaacgttga taacgccgtc gggaacacct gcttccttat
    12181 aaagctcggc aattctgaga gccgttctcg gagtaaggct cgctgccttg agaaccatcg
    12241 tgttgccgca tgcgatacag gtaggagcca tccagccgaa cggaatcata gcaggaaagt
    12301 ttaccggaac gatacctgcg aaaacgccca tcgactcgcg gtacttaact gtgtcgtagc
    12361 ctcttgaagc gtccataacg ctctcgccca tcataagtga cggaccctga gttgcaagct
    12421 cggtgccctc tttagccttg agaacgtcgc cctcggcctc agcccatacc ttgccgtttt
    12481 cttcggcaac aagcattgta agctcgtcca tgtgctttat aagaagatca cggatgttgt
    12541 aaagaatctg tgctctcttg atcgccggag ttgagctcca gcccgggaaa gcagccttag
    12601 ccgctgcaat agcgccgaga acctcgtcgt tagtacagca gggagcataa cccgttgtct
    12661 cgcccgtgct ggggttgaca agagtgtagt atttgtctgt ctttgattca acgtactcac
    12721 cgttgacaaa gtatttgagt ttttctgcgg acattgttac atctccttgt atgttattct
    12781 attttttaac ttgtttttca gacctctgct ttgagtgtaa gcctcggtcg gtttctgccg
    12841 gcttttcttg cctcccccta aaggggcgcc aagaatttct gctaactttt aaacagaata
    12901 atcaagctta gtctatctgt tcaagccttc cctttgttga ttcaagggga ggtgtcgcat
    12961 tagcgacgga ggggataaag atatgttctt acttatccct tccaccgcaa gcggtccccc
    13021 tccccttaca ataagggtag gcttgcttga ctttcacctt aattattcac tataaattat
    13081 actcaaagtc ctgccttttc tgcaatgtag gctcttgcca tctttgcata cttgaacgga
    13141 tttgcgattg caggatcctg ctcagcttca acgagcatcc agccttcata gcctgtttcg
    13201 tcaagaacat caaaaatcgg cttgaagtca attgcgccgt caccgggcac ggtgaaagca
    13261 cccatacgaa cgccctgaag gaaagacttg tgctcgtcct tgacctgctt aacaatctcg
    13321 ggacggatat ccttgaggtg aacgtgcttg attctcttgg catacttctt gagaactgcc
    13381 atgtagtcct cgccgcaata tgcaaggtga cccgaatcga aaagaaggta aacggcgtcg
    13441 ggatcgcaca tatccatcat cttgtcgatt tcctcggctg tctgaacaac cgtacccatg
    13501 tgatgatgat aaacaagcgt aatgccgtat tccttagcaa tgttgccgag ctttgaaagt
    13561 ccgtctgtga acttcttcca ctcctcgtcg ttcataacgt atttattctc gaaaacaggt
    13621 gtatccatct gtccctgaat gctgtagctc tgctctgaag caccgattct ctttgctccg
    13681 cagcccttaa gaaattcgca gttctctctg aaatccttct caacctcatc aaaaggcttg
    13741 gtgagaatga atgatgaaaa ccagcggtta gcgatctgaa gtcctctgat atcaaggtac
    13801 tcgtgcattt cctcaacggt ctttggatat ttgtttccta tctcacagcc ctcaaagcct
    13861 gcaagtgcca tttcacttac acactgctca aaggtgtttt cactgccgag atcgggcata
    13921 tcgtcgtttg tccagccgat cggtgcaata ccgagtttaa ttttttttgc gtccatgtta
    13981 cctctccttg ttaaagaatt gcgtagtaag tttttgtaag gtgttgcttt aaggcaatat
    14041 cgcacaattg aggtgtgcga tttgcgtagt aagatttttc gtcaggctgt gcaaaaattt
    14101 tgcaggcttg ccgtcacaag tcaagaaatt tttgtgcggc atgacggaaa tagattcaag
    14161 caaggcgtgc gccgcaattt gtgcggtgtt gccttaatac tttttagcct cggaaagctt
    14221 ctgcgttctt tcggcaagtg cttccttacc cttttcagta cgcggaatat ccgaacagcc
    14281 gacattccac cagccgccgt atccgtcagt catggatttc ggaagaactt ttatatcaat
    14341 gagcacaggc ttagtctgct taagtgaatc aagcaccgcc gcctcaagct catccatagt
    14401 ctttgcggtg tatgcgacat agccgtagcc tcttgcacat gcggcatagt cgatattcat
    14461 aaattcgccc tcacgaatcg gcttgtcgcc gtcacggtaa cgagcttcgg tacagagcga
    14521 attaacaccc tgacccatct gaagattgtt gatacaaccg aaggaagcat tatcaaagag
    14581 catgacgttg atcttcttgc cctcctgcaa tgcagtcagc atctcggaat gaagcatatt
    14641 gtagctgccg tcaccggtga aagcgtaaac ctcctgatcg gggcaagcga gctttgatcc
    14701 gaacgcacct gcgatttcat agcccatgca tgaatagccg tactccatat tgtaggaata
    14761 gagcttgtct gtcgtccaca ttctctgcat acagccggga agcgaacccg ccgcaccgac
    14821 tgcaacagca tcggccggga tgagcttgcg aataagcgag agagccgccg tctgagtgat
    14881 aacaccgccc gttgctttaa cgaagctttc aactgcgtcg ggcatatgat taggaatttc
    14941 gggagtgtag ccctcgccgt agttagttgc ggcaagacgt gccatttcct tatcccaatc
    15001 tgcctttgcg tctgcaattt cggtcgtata agccgacttg tagccatcaa gatatttttc
    15061 gagagctttc acaccctcct tggcatcggc aaccatcttt atgctgtcaa gcttgcctgc
    15121 gtggaatgcg ctggcattga tcgttacaac ctgagtgttg gcataaagcc actttgacga
    15181 ggttgtgaaa tcggagaatt tcgttccgat accgataatc aaatctgcgt ccccggcaag
    15241 cacgtttgcg cttgaattgc ctgttacgcc gataccgccg acattgagat agtgacttga
    15301 ttcgattgca ctcttacctg cctgagtctc ggagaacggg atgttgtgct tctcgcagaa
    15361 agcctttacc tgctcgcctg cgaacgaata tctaacaccg ccgcctacaa taacaagggg
    15421 cttcctgctc tttttgattt cctcggcaac cgctttaact tcgtagtcgc acggaatctg
    15481 tcggggaatg aaatgtactc tttttctgaa aaattcagcg ggataatcgt aactttcacc
    15541 ctgaacgtcc tggcagagag cgattgccgc cgcacctgtg tgggcagggt ctgtaaggac
    15601 tctcatagca tttgtcagag ccgtcataac catctcggga cgggtaatac ggtcaaaata
    15661 acgtgttacc gctctgaaag cgtcgtttgt cgtgcatgtg ccgtcatgga gctgctcaac
    15721 ctgctgaaga acggggtcgg gctgtcggct tgcaaatgtg tcgcccatga aaaggagaag
    15781 cggaatattg ttgaccgtgg cagtagccgc cgccgtaacc atattcgccg cacccgggcc
    15841 gattgacgac atacagggta tgattttaag tctgttgttc atcttagcgt atgccgttgc
    15901 agcctgagcc ataccctgct catttctgcc ctgcataacc tttattgagt gctctgcctg
    15961 agagagagcc tcgccgacgc cgagcacaca gccgtggccg aaaatagcat agaaaagctc
    16021 aacaaatttg ctttcaacga gctttccgtc ctgctcaatg gcaacgtact gattgtcaag
    16081 atatctgacg attgcctcac cggcagtcaa tctgattgtt ttcatatctt atgatccttt
    16141 ctttgaggat gttggggata gacgttagat gttagattgt agatattaga tgtctgtact
    16201 ttcagtgctg tcttttctta ccccaaacga aaaatgtgct gtgcagaatt cgccaaatgt
    16261 attctaccga accaaactgc caaaacgcat tttagcggga gatttttgtt aggcggattt
    16321 tattccgtcc tacaagcaaa cctctgaata aatttgagaa aattttgttc acagtgcttt
    16381 ttgcagttcg ggagctgtat gtatatactg ccccgaacga aaaatgtgct gtgggcaaaa
    16441 ttagccaaat ttaagcgcgg gctaccattt cgccgtactt atccttttct tctttgataa
    16501 actttttaat ttcatcaacc gtcggcatga agagcgagca gccgtgagcg gcaacaagca
    16561 ttgaagccga tgcacttccg agctcaaggc agtccatgat gtcgagacct tcgagaagtc
    16621 cgtaaatgaa tgccgaaccg tagccgtcac cgccgccgaa ggacttaaac gccttgaccg
    16681 ggaacggttt gattgaatac tcgccgtcgg ccgtgtgagc cgttgagcct tccttgccgt
    16741 gcttgatgat aacgatttta ttacccatct tcatccagtg ctcggctgtc tctctgtcgg
    16801 ttctgccggg agcggaaata gcctcggtaa ggtcaaattc ctcacgggaa cccattacca
    16861 tgtcactgtt ggaagcaacg attgaataat aaattgcgat ctcgtctgca tttttccagt
    16921 tgtaaggacg atagtcgata tcaaaaataa tctttgtgcc gaccttctta gcaaggtaca
    16981 ttgccttaag gcaagcctct cttgacgggc tttgcgaaag ggctgtgccg ctgatgagaa
    17041 tagcctttgc gctctttatg tagtcctcgt caacatcgtc cgggtggagc ataaggtctg
    17101 caattccgtc acggtacatg aggatgctgc tttcgtgctc cgacttaatc tcggtgaatg
    17161 taagaccgag cttttcgccg ccctttgtgc gagtaacgtg ggaaatgtcg attccctcgc
    17221 ctgcgaagaa atcctcaaca tatgtgccga actgatcatc ggaaaccttg ccgatgaagc
    17281 cgaccttctt gcccaagcgt gcaaggccta cagcgatgtt ggcaggagaa ccgccgacat
    17341 atttcttgta gttttcgcac ttgtcaagcg tgttgtagta gtccgtcggg ttgaagtcga
    17401 ttgcaactct gccgatcgga atgaggtcaa gctctcttga ctcgtcaaaa tcaatgtatt
    17461 tcatactgaa acctccgata atatattcaa taaataatta accgatgtga attccgcgtt
    17521 cctctctctt ttcggcaagc tcgagcgtaa tcttttccat ctttttgccg acaagatcat
    17581 atgacttcat cggaatcgtg ctgagaagaa ggctgatgcc cggaacaata gaaataagtg
    17641 agaacattgc atatctcata gtcatcgaaa gatccgttgc ggcaacggct gcttcctttg
    17701 caagcatttg ttccggatta agaccgattg caagatacat aactatgata cctgccgttg
    17761 cgagggcatt gccgagcttg ttgacgaagc cctggcatgc cgagccgaga ccggtttctc
    17821 tgtagcctgt ctcccactcc atatagtcaa ggctgtcggc aatcatcgca tatgatacga
    17881 cgttgagaac accaagcgga accgcagaaa taatgaggaa cggaatgcag ataaagaagc
    17941 tgagttttcc tgcaacaacg aagtagccga ccaaggaggt caaaagactt gcaacgaagc
    18001 ccaaggtgca tgttgttaca acaatctgtt tgtaattgaa cttttttata agtgcaggca
    18061 taaagagcat tgcaccgaac attccgactg ccgagcaaac cgaaagaatc gtgctgacgg
    18121 ttccgatact ctttgaaata gcttcggatc tttcaagtcc cgtcattgtg ctgaggtctc
    18181 cgccgatata taccgcataa cgtgcaacat gaaccgaagc cgcctgaagc atatatctgc
    18241 cggatgaaag aatacccatg acgataacaa gcataagcgg cttgcatttg aatattctcg
    18301 aaagctgtga gggctctccc ttgacggatt ttttatcggg aataacaaca cgctctttaa
    18361 cgtgcggata tgaactgaca taaagaataa cgccgattac cgcagcaaac acggcaatca
    18421 atatgtattt cattttatca tagtttattg tatttccgac aacaaccgtg aatttattga
    18481 agaaggtctg aaccaaatcg ttatccgacg cctgcatctt agcgcagagt cccgggagaa
    18541 ccaagcccgc aacaagatag agagccatag gaacagccga tccgataccg ttaaaggtac
    18601 gggcaacgga aataaggttg cctcgctcat tcggattggg agtcataaca ttcgggagac
    18661 tccagaaagg aacgtctgcc gctgtgtaaa tcatgcccca aagaacgtaa acaacgcctg
    18721 cataaatcat caggtctgtt ttgtcaaggt cgggataata gaacataagg aaggtaagta
    18781 acgcaatggg aaccgacatg aacacaatat acggcttcat cttgccccag cgtgttgtgt
    18841 ggcggtcaat gatcataccc atgagaggat cattgatggc gtcccaaact cttgccaaaa
    18901 gcatgagcag aagaacaaaa ataagaggga gcttcataac gttggtgtaa aagtccgaga
    18961 tgtaggagga cattacactg tagatcatgc cctgtccgag tcctgccaga cagaacatac
    19021 gaaactcttt ggattcgaca aattttccct gcatcacgtc gctgtgggaa gcctttgacg
    19081 cagctttttt cgaagtgctt gccatataaa aactccttga tatcaatttg acagataact
    19141 ataattctat ctttgctatt ttaacatata ttgcagtatc atgcaatata aaatattaaa
    19201 tttatacaaa aaaataagta ttatataaca agcaaaaacc ttcccctttt tcaagaggaa
    19261 ggtaatgtgc gtatttaatt aaaatacaaa gtttgtgaag tcaacagatg tctttgcgtc
    19321 acctgttgca gacttgattg taactattgt gtatttaacc tttgtaagag caacatccca
    19381 gttataggaa atgtggagcg agacaagatt gcctgtcttt gagttgatcg ttgcggtgat
    19441 cttgatattc ttgttgttct caacaacgct ttcaacggaa gcaccgtcgg cattattaag
    19501 gccgtcaaaa atattgcccg cattcttata atcataagcc ttcttgtcgc ctacgcccga
    19561 gtagagaccg gttcttgcaa gagctgttga atcgctctta cctgccgagc ttgccttgct
    19621 tgtgccgttt ttaagggtca tggtgataac gtaattatcg ccgctctgct tgcatgtcgg
    19681 tgaagaaata tcgtttgcgg aaagagaagc ctttgaaatc tcgccaacct tgccctttgt
    19741 gttcttatag ttgatagtac ctactccgag gaagtcgtta actgtatcaa caactatctt
    19801 catctccatg agctttccgc cgttgaggtt gctgatagaa gtaactctct tctttgagaa
    19861 gcctgccttg gagttaactg ccttgttaac ggcagcattg taatatgctg cgatgtcggc
    19921 tgcagttgaa ggaaccttgg aagctgttcc ggatttgcca ggctttgatg ccgacggagt
    19981 tgccggagaa acgggatttg acggatctgt tgccgaagga tctgcagcat ttgacggatc
    20041 cgttgccgaa ggatcagcag acgggtctgc cggagcagac ggatcagccg aaggattaga
    20101 agacggatct gtcgacggat caccggaggt tgaagaattt gccgcagcag ccttagcggc
    20161 gtcaaccttg gccgtgctta tcttaccaac gctgattaca gatgtaactg caactgcgat
    20221 aacgcaaagc aacgcagtca cacactggat aactgtcttt ttcataagtt acttacccca
    20281 attcttttaa aattcgttta gatttgtcat agacgggtgt attatatcac atatttttta
    20341 taaaatccag tatatttata cattttttga aattttttat ttcgagctaa aaattcatat
    20401 ctttttcata gatttttatt tatatgtatg ctataataca ttttgtgtgc ggaatggggc
    20461 tatttaaaag ttcttaaact tttacatacg ctcccctaca ggggtgccga gaagagtttc
    20521 ttccggtctt
//
LOCUS       DAWBSW010000010        20529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_571_length_20529_cov_2.628456, whole genome shotgun sequence.
ACCESSION   DAWBSW010000010 DAWBSW010000000
VERSION     DAWBSW010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 20529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20529
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_20529_cov_2.628456"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10692..10701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13906..13915
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17025..17072
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5407 a         5022 c         4043 g         5989 t
ORIGIN      
        1 tttgcgtacc tcggcattaa gtctcttggg caaacggctg ttcattttat tcagcgcttc
       61 ctcaacggga aggtctccct tatccatcag cgcccattct tttgaacgga aggtattcgc
      121 aagaacagcg tctctgtcct cgggagcaaa gtgattgctg accgaacgaa ccgggtcaaa
      181 atcaatcaga actccgccca tatcaaatat aatatttttt atcattcaac cgcctccgtt
      241 ctcaaattta ccgtcagtcg gattgctact aaggtattat ataacagatt cccgtaaaaa
      301 tcaatttagg ttggttaaaa ataaatgttt tcttcaaata gttatataac aatttaacta
      361 tatctgcaat atagtagttt taatgtataa tatatatgta agattatgga caataacgga
      421 gaaatcacaa agaataacag tgaaataggt tctttctttc ttaaatacaa ttcataactt
      481 atagtttttg cacccatgca gtatggacac gtgggtgcgc ttttattttc tatgacaaaa
      541 tcttaatcaa aaattaagat ttcaagcgtt tgaagctctt gaataagccc gtaatttgtg
      601 ttaaaatcta ttgtacttgt catgataatc caataaaaac atatgtctgt gaatacggag
      661 gaaatagaat gaaggcaaaa gttaaataca ggaaatacgg aaatattccc gacatggaca
      721 ccgtcaagga tattattata tatggcgcaa agcaaggcaa acataacaaa cagtatgcct
      781 ttaagaatat gaatggcgaa tactgtgaga agacctttga tgaagttttt tatgacacaa
      841 cgggacttgg tcagcatctt tacaccctcg gtttaaaggg taaaaaggtc gccattctca
      901 gcgaaaacag ctactactgg atagcggctt tttattctat cgccagcgga aatatgatcg
      961 ctattccgct tgacccaaag cttccgaaag aagatcttgt cgatctcatg gtaagaagcg
     1021 gatgcgacgc gatttattat gcccgggatt ttgaacctgc aattcagctt atgaaagaaa
     1081 atcccgatgt caagctttcg ttttatctca agctcgagga ttttgatgag cttgtcaaaa
     1141 tgggacatga tgagcttgac aacggagcga aatattatct cgatgacgag gttaagcccg
     1201 atgaccttgg atttatcgtt tatacctccg gcacaacggg caagagcaag ggcgttatgc
     1261 tctctcaaaa gaacgttgcc gtcgatgcaa tagcaacctg ccgggcgatg acggcagacc
     1321 gaacagttgc atttcttccg tttaaccata ctttttcttg ggcaagtgca ctttttgcat
     1381 gcctgctgct ttcaaagtgg ggctatatat gctcaaatct gagaaatctt cagaaggata
     1441 tgtatgccta tcatccgcag cacattacgg ctgttcctct tgccgttgaa acaatctata
     1501 aaaagatttg gttcaccgca cgcaaggagg gtaaggaaga aaagctcaaa aaaggcatca
     1561 agctgagcaa tgcgctaatg aagatcggta ttgatgtcag aaggaagctt tttaaagaga
     1621 ttctcgacaa cctcggcgga gatcttgaaa tgataatctg cggcggtgcg tctcttgata
     1681 caaaatatga gaaaggtctt tatgagctgg gaattcaaat tataaacgga tatggcgcca
     1741 cagagtgttc gccgatcatc acctgcaaca ggctcagcaa ctataagctc ggcagtgcag
     1801 gctatgcgct tgagtgcaat gacattatga taaaagatcc tgacgaagag ggcatcggtg
     1861 aggtctatgc acggggctca aatgttatga tgggctatta tgacgaaccg gaggcaacag
     1921 cagaagcctt tgacggagat tggttcaaaa caggcgacta tggctacatg gataaggacg
     1981 gcttcctttt cctaagaggt cgtaagaaaa acctcatcgt tctttcaaac ggaaaaaatg
     2041 tttcacccga agagattgag gacaagcttt tgacggtgga ctacataaaa gaggtcgttg
     2101 tctacgaaga aaacggtgca ataaccgctg aattctttct tgatgagaat gaacccgacg
     2161 caaaagacag actcaaaaag gatgtgcgtg caataaatat gcagcttccg acgtataagc
     2221 aggtcacaaa aaccaagatt cgtgacacgg agtttccaaa aacaacaacg cttaaaataa
     2281 aaagaaaata ctgattgcta aaaccgcttg agcgataaac tcaagcggtt tcctgttttt
     2341 acgcaacgcc tcaaaaacga ggtgtgtatc attatttgca ggtgttgcct taatcctcaa
     2401 acgggatttt catattaaga tatctttcga atctttccgg tgagcgttta taataacgtt
     2461 cccctctgtc aatcgcaccg atgttaagca tctcactctc tgtcagttca aaatcgaaga
     2521 tatctatatt ttccctgata tgatcgggag attttgatcc ggggatgacg atattaccct
     2581 cctgaacatg ccagcgaaga attatttgtg catttgtctt gccgtatttt tttgccagcc
     2641 ttgtaaatat cggctcgctt atcagtttgc tgtcgccgtg accgagcgga taccatgcct
     2701 ggattcgaat atcctgaaga tttaagaatt ttttcagttc ctgttcaccg gagtactgat
     2761 gtaattctgt ctgaagcaca gccggcttta ttgaacaaac attcatcatc cgacacattt
     2821 cctgctgagt aaaattcgaa aggcctattg atcttacctt gccctcccga taagcttttt
     2881 caagcattct gtatcccgag atgaaatctc ccgtcggctg atgaatcagc aaaaggtcaa
     2941 tatactcggt gcccagtcgc tcaagggttc tgtcaatcgc attctcgtcc gtgtaatgag
     3001 tcggccatat ctttgtttca agaaaaattt cgctgcgttg cagtcccgaa tctttcattg
     3061 ccctgccgac agccttttcg ttggcataca tatttgccgt atctataagt ctgtaaccgc
     3121 attcaagcgc agcaaaaacg gacttttccg cttcatcggg tgaaagcaaa tacgttccta
     3181 ttccgaaaat cggcattgta cggccattgt aaagcttaac atattccata ataccgctcc
     3241 tttcaagcaa tcacccatct tcttttcaaa agtaattata acacctaaac ctgactttat
     3301 gtcaataaaa aaattgtaat aattttataa aaaaacggca acagaattgc cgcttttatt
     3361 acaatataag ctcggggaac ggcacaaaca ttttaacgtc ctcgtcatgc tccccaatca
     3421 ttttttccat ccacaccatg tcgtaccatg tgtcaaactt tctgccgcat ttttcaaacc
     3481 gacctacggt tttatagcct aggtgctcat gaaaattgac gctgttcata gtcaaatgct
     3541 cgtcctcgac ctccggataa ccgatgcagg catatagatt tataacattc tgagccacag
     3601 atatttcttc aatcgcagca tagagccttt tgccgattcc tttattgcgg caaccttcgt
     3661 caatataaat tgacgtttcc gccgaatgat cataagctgc acgccctaca aatgagcctg
     3721 tgtaagcata tcctacgatc ttgtctccga cctcggcaac aaggtacgga tatctttcca
     3781 gtgtgttctc aatccgcttt ttgaattcat gcactgtcgg cacgtcatac tcaaacgtaa
     3841 tagccgtttt ctcaacataa ggtgcataaa ttttcaatag cttctcggca tcgtcgggtg
     3901 acgccacacg aatattaaca ttctccattt ctgtacctcc gcttatattc cgaaatgaat
     3961 tacccattta tcaaacatca ctgccgtttc aagaagcgtt ctgagcttat caagctctgc
     4021 cgaattttct atcgcttcaa taaagctccg ctccgaggtc ggcggaatca acgttatacc
     4081 gcaatatgca agcccttttt tcggctcatc aacaacatgg tgatagaact ctatctcggc
     4141 aagctttggt aaaatacctt caattgcatc gtcaggcacc gctatgcacg catatttctg
     4201 cggctcgtac atatcaaacc tcacgccttt ttgaggctca tgcggcatca tcgcaaattc
     4261 atggaccgcc attcatatca ctcctatatg aaatatcggc tgcagctttt tcgccgcagc
     4321 cgtttaatcc ttcggttact tttttaatct cttctcaggg cttcgatcgg cgtcatttga
     4381 gccgcgcgct ttgcaggata aattccgaag aaaattccta ttgcgcttga aaatcctact
     4441 gcgataagaa ctgttgtaaa tttaaccgtt atcggtatgt taatataagc cgcaaccgcc
     4501 catgcaccgc cgactccgag cacaaagcct attgcaccgc cgagcagaca cagaattacg
     4561 gattctgtca ggaactggag catgatggtt gatgtcttgg ctccgagagc ctttctgata
     4621 ccgatttctc ttgttctctc ggttaccgaa accagcatga tattcataac gcctattccg
     4681 ccgacaagca atgatatcgc actgacagcg gcaatgaatg tggtgaaaat gcttataaca
     4741 gaatcaagca aatcaatata tgtcgccata atgctaagcg aataaaccga atcatcaaag
     4801 ctgccatgtc ttgtttttat gagattgaga gccgattcgc ctgtgttatc aaggtctgcg
     4861 tcgttgcttg ccatcagata aagctgcgta tacctattca ctttgtcagt aagctcgccg
     4921 ttgagcgatg cgggtatcgc aagcatttcc atgctcattg cacctgttga ataagttgac
     4981 atcatgcttt ccagttggtc ttgcgatacc atggaatttt ccgtcatatc aacaacgccg
     5041 ataattttca gggaaaccgt cgtattattt attgtataat ttatcgtttc tccgacacag
     5101 ttttctttgc caaaaatctt tttagccgtc attgtgctga tgatgcaaac atttcgcttc
     5161 tggtcatatt cgtccttagt ccatgatctg ccgtactttg ttgtcatccg attgataaaa
     5221 agcaaatcct cgttgcccgc aattgtgaaa accaaaccgt catatttgcc tgcctgcgcc
     5281 tgaccgaatg agaattcata aggtgaacaa tagctgaccg tgccgagctt ttttattgag
     5341 gaaatatcct cctcggttat atagtttgac tggttggcag tattggcatt gacggaaatc
     5401 tgaatggcat ttgagcccat atcctcaatc ataccgacaa tataatcacg gccaccgttg
     5461 ccgatggtta taatcgaaat aacagaagca acgccgatta tgataccgag catcgtcaaa
     5521 agcgaacgca tcatatttgt ttttatactg aaaatcgcaa tccgtatatt ctctctgata
     5581 tccacagcag tcctccgtta cttcagctta accttttctc cgtcagcaag cccgcttgtg
     5641 tttttgataa gaatatcgcc ctcttttact cccgaaagaa cctgataata ggtatcattt
     5701 gagattccga gagtgacctt cttcttttct gcggttgatt tctcctgatt atatgcataa
     5761 acataaacgt cagagccgtc aataattatc gcttcaacag gaacgacgag cgcattttcc
     5821 tgagtgtccg taattattga gatatcaacg tctgttccga ctatgaatga cttgtcgccg
     5881 tcctctattg taacttgggc cggaattgtg tttgagcttc cgctgcccga cataattgaa
     5941 ttgatatcaa acgagctgcc ggtggatgtt gcaattgctc cgacataaga taccctgccc
     6001 tcatggactt cattatttgc cgattttacc gaaacctctt gattaaggct cacatcaagc
     6061 acatcatatt tactcagcga aatatcggca acaagaggat aattgagaac ctttatgcca
     6121 accttttccg agtcgaagaa agcgcttatc atctttgaaa catcaagccc cgaggagacg
     6181 gaagaaatga tggagctgat atcaatattc ttgccgtcaa cagcggtctg aacctcaacc
     6241 gtttcgccga cagtaatatt aacctcactg acaagtcctt tttcctctgc aatccagccc
     6301 tttttcattt catttgtctg agtaagagcc ttttgatatt ccgactgtgc gttttcatat
     6361 attgttttat aaatcgaaag gtaggtatcg tttgactgca attcaagcgt tgcgagctga
     6421 gccttgagct gaacaagtga catttcggca ttgataagtg cacttgagcc gccgctcatc
     6481 ttagcaaaag cattcatatc aaaggaaccg gagccgcctg caatctttcc gaggttgtcg
     6541 agtaaaccgg aaatatccga gctgctcgat cccgccgaaa gaagcgctgt gagtaccttt
     6601 tttgctgttg cctcgtcata ttcaacaccg aaaagctttg cgacttttat aaatctcttc
     6661 acaaggtcat cagagacctt tacacctgcc gaatttccgg atgacgacgg cttatttttt
     6721 gatgcgtttg atttcgcctt gagcgtttct atctgctttt caaggtcgga aatctgcttc
     6781 tttgtgcttg caatactctt gcttgcagag gatgcctcat tttttgcctt attatatgcg
     6841 gcaagagcct tttgatagtt gttttctttt tcattcagtg ccgcttcaag cgaagaaaca
     6901 tcgaaagtag cgagaacatc tcctgcttta actgtatcgc cgaccttgac gttgactgtc
     6961 ttgaccttta ccttgtcaat cagcgcatag gttttctccg aagcggttga aacagttccc
     7021 gttgtgtcaa gcgtttcgtt aatcgtctgc tttgtaacct cgacagtgct gacagcaagc
     7081 ttttcatcgg gcttgaaaat catgtaagct atggccgaaa ggacaaccac cgccaaaatc
     7141 gagcagagga taataacttt tttgtttttc ttctttttct gctgcataaa cgcacccctt
     7201 aactttatat ctatatacta atcaatattt tatcgggaga acgacatgaa acccctcaaa
     7261 ataatatttc ctttgttaat aataattctt ctgacctcct gcgtgggtca gaaacaactc
     7321 aatattgacg agctgctgaa aaaatacaac agcataacgg gaagcgagct ggaaagctca
     7381 atgtttaccg tttacaagag cggcaatgat tttgtttaca aaagaatcga aggcagtgca
     7441 ttgctttgcc tttatgccgc caaagacggt gaaatcaccc aatgcaccgt cactgtgaaa
     7501 agcaacgata agaccggctt cgaaccgctt tgcatatcgg taatcgaagc tttaacaggc
     7561 ttttctgcca aagagagtga agattttttt aaaagtcccg gtactttgaa aaattataag
     7621 ctgacaatca ataattacgg aatcggcaag accatgatcc tgaatagaat tgaaaatgaa
     7681 ctgaacacca acagtatgcc gaccttaaaa aggaaagtaa aagaggagga tatagcacgt
     7741 cccactcttt ccgacactga taatttcact aaaattatta gacaataaaa ataatgcgta
     7801 caaattaaat aattgttaaa atttggttaa taaagcaaaa aggtgatggt tttccatcac
     7861 ctttacactg cattttaaat agaaatcaac caacgaaacc gcatgcggga atctctggtt
     7921 ccggctcctc cgtaggagtt tccggctctg ttgttgtctc attttcaggc ttgtagtaaa
     7981 gctcaagagc cttgttagca ataatgctgt ttgaaatagc attattaaga gcttctacgt
     8041 ccacgccggc atctgtaagg aatgcatcag cgatatcacg aagtgcgccc ggatccttaa
     8101 tttcatcgga accgatgccc ttatcgttga tgtacttctc gagagttgca acgaggtcag
     8161 caacatcttc ttttgaagcg cccgtcttaa tagcgtcaac aatgtcattt gcaatatctt
     8221 ctgccttttc gtcattctgg agagctgaaa cagcaccttc gattgttgaa gttgttgtct
     8281 cgggaacatc ttcagctaca tcgttttcag cacgaacaga gaaagcaaca gttgcgcaga
     8341 cagcggctac cataagaatt gccaagaact ttttcatacg aattcatcct ttcataatat
     8401 ttattagcat gagtattgta acccataata atacaaaagt caagcggttt aagaaaaatt
     8461 agaaaaaagt ttatacaata aatctgtttt atgaatgagc cttactgtaa tcataatttt
     8521 tccagcaaat cgtaacggtt ttgccgctga ccgagtatct ctcaacattg tggtaattgt
     8581 tgattgagcc ttccttgaag ccctctttct caagtccgtc aatgaataga ttcatcattt
     8641 tatctgtcgg gaattcgatt gttgcaacca tcacgagcgt cgtctggtca cggtgctttt
     8701 caagcacacc cgtctcgaag tccgctgccc accagcaata tctcttgccg tagtcaatct
     8761 tctgaacagg agttgaatcc gcagctttct catactgcca gagcttcatt gaaacaggca
     8821 ttgcgtcctg atcctcaaca cacttgtaaa gaagattatt gggaccgaat ttatcaagtg
     8881 cggtctgatt gtggtctctg taataaaaac caacctcaca gccgaccgta gccatggcat
     8941 attcgccctt ccaaagctca ataagccagt ccttgtcgtt atatgtgaaa tagattctga
     9001 tggttgaaag atagaaatta acaaacttgg cgccccagtc ataagcgccg ttaaatccga
     9061 actttctctg cggagcaaga gcattgtaaa ctacattcgg cgaggctgtt gtcgactccg
     9121 gttttgtagt ggttctcttt gttacggttt tctttgttgt cgtctgtctg tttgtcgaag
     9181 cgctcggagc tgtattgctc ggggtaatat tgcttgccgt gtacttgttg ccggactgat
     9241 tcgggttata aacgaccgaa tgaccgttgt tgtcaacaat aaaagactga ccgtcatcgc
     9301 cgacaacgat ctgtgtcggg ccctccagaa tgtcgttgcc ctgtccgatt tgaatttcgt
     9361 ccgaactgcc ggcagacttc ttctgagtaa ttttcttaac agagccgcag cccgtcagca
     9421 caacgctgaa tactattatg aatgaacaaa gcaaagctat gtaacgtgtt tttgagtttt
     9481 ccttcttcat tttacacaat ctccattata tttttttaca ttgtatcaca gattcaaaaa
     9541 aattacaata cccaaaaatt cccttatata tccgtcgtat aaaaaaaggc tgccgcactc
     9601 aacgacagtc cttctgttta taaattacct gcattcgcga gcatctctct tgcgctctca
     9661 agctggagct ttgtaatctc catgccaccc ataattcgtg caatttccgc aacacgttct
     9721 tcctcgatga gcgacttaat gtttgtatat gtctttccgt cggaaacaga cttgctgata
     9781 taaaggtgaa cgtcgccctg agccgcaatt tgcgcaaggt gtgtaacaca gatgacctgt
     9841 ctgcccttcg atgcgcttct gagcttcatt gcaatcttct gcgctgctct gccgctgaca
     9901 cccgtatcaa tttcatcgaa aatcatcgtg tcaacaccgt cggtctcggc aagaacattt
     9961 ttaatcgcaa gcatgattct tgaaagctca ccgcccgaag caatctttgc aagaggcttc
    10021 ggctcttcac cggcattggc tgaaatcaaa aattcaatct cgtcttttcc gttttccgtc
    10081 aagccgatat cgctgcattt cacgacaaag cgaacattcg gcatatcaag aaattcgagc
    10141 tccgagcata ttgcattgct gagcttttcg cccgccgttt ttcttgattc ggtcagcttg
    10201 caagccgccg tttccgtttc ggcgagggct tccttttcct gcttttgaag ctccttaact
    10261 ctttcctcgg aaaaagctat gttatcaagc tcggctctcg ccttttcaag atattcgagc
    10321 atttcctcct cggtgtcgcc gtattttttt gaaagacgat agagcagatc caacctttcg
    10381 tctatatcct caagctcgga cggatcaaaa tcaacctccg tcattgcgtc acgcaccgtt
    10441 tcggcgatat cctcaagctc gtatctgata tccgttattt tctcagaagc ttcaccgagc
    10501 atctccatat aagcactcgc attcgctatt tccgattccg catcggttat catgtcaatt
    10561 cctgctccgt cggctttcaa ggcttcatag gcgatgttaa ggctttcgat aaccttctgc
    10621 gaattattga taagcgattt tcttgccttc agctcgtcac gctcaccgat tgttatttcc
    10681 gccttttcaa gnnnnnnnnn ntttggtatg ttaaaaaatc aattctttgc gacttggagc
    10741 tttcatcggt cgtcagcttt ttaagcttgc ttcttatctc gcaaagcctg ccgtattttt
    10801 ctctgtaatc cgcaattaaa tccgcattgc ctgcgaagct gtcaacaaag ccgcaatgct
    10861 tttcgggaga aagaagcgac tggttgtcat gctgaccgtg aatgtcaatc agctcgccgc
    10921 cgatttcacg aagcatcgag accgtcacgg gtgcaccgtt gatacggcag acattttttc
    10981 cctcacggct gagtgtcctg ttgattatca gcacaccgtc ctcgcaatca attccgttct
    11041 cgtcaagaaa attgcgaact ctttcattta catcctcaaa gacggcataa acctttgcac
    11101 tctgcgctcc tgttctgatg atatcacggg aagtccgctc gccgaggata gcatttatcg
    11161 agtcaatgac aattgatttt cctgcacccg tttcacctgt cagaacatta agtccgcagc
    11221 cgaattcaat ggcagcactt tcgattattg caacattttc aattttcaga cttgtaagca
    11281 tattatcaca tcctcaaatt gtgcggccga tttatttgac tattctgtcg atagattctt
    11341 tcagctcgtg ggcggtagct tcggttctgc aaagagcaaa aatcgtgtca tcgcctgcaa
    11401 tcgtgccgac aaggccttcc cactccatgt tgtcaaaggc tgcgcacgcc gcattcgcca
    11461 tgccgacatg gcacttgata acaaccatat tccctgcata atccgaggaa acgaccgaat
    11521 gagaaaaaat cgttgtaaat tttgaaaggt attcatcggt ctcactcttg ccctctacat
    11581 agcggtaaac gccgttctta tccatctgct tgacaagtct caactcttta atatcgcggg
    11641 aaactgtcgc ctgagttgag ctgaatccgt tctcttcaag cttctcaagc atctcctcct
    11701 gagtgccgat cgcgtacatc tcgataagct tgagaatcat actgtgtctt ttctttttca
    11761 tgacattttt atctcctttt acagccgcct ctgctccagc ttgctgttaa gtatttcaag
    11821 aaacgtttcg ttctttattc ttataaattc gccgaacata tcggctttgg aaattctgac
    11881 cgagcatccg ctctcaatcg gaattgagtc ctctccgtcg caggaaagac cgatttcgac
    11941 gtctcccttg ccgtgaagaa taaattccga atcaggctta aaaatcaacg cgcgggaaaa
    12001 aagggaatgt gtgcatatcg gtgtaagcaa tatgctttct atctgcggat cgaccacggg
    12061 tccgcccgcc gaaagattgt atgccgttga acctgtcggc gtggaaaaaa taattccgtc
    12121 cgccgtgtat ttattgagaa actttccgtt gtgctcaaca acaaaatcgt ccatctttat
    12181 ctggaagcct cttgcaacaa ccgcatcatt gacgcaatag tgaacgctga gcaccctgtt
    12241 atcgggtgac ataatttccg ctttcagcat catgcgacgg tcaagctcat attttcctct
    12301 cttgagattg gaaagcatat taagctccga cggctcaaga cctgccataa atgcaatccg
    12361 tcccgcattg ataccaagag tcggtttgcc gaatttcgcc gcgtgaatta tagttccgtc
    12421 gccaccgacc gctatcacaa tatcgcagat cttaatcatt tcatcaacag tgatgaactc
    12481 ggcttttgta tccttgaatt catctttata atcacgcttt atgcaatagc tcatactgtg
    12541 cttttcaagc tcagcgcaga cctcaagcga tacctgcaaa gcgttttttc ttgttaaatt
    12601 aggaagtaac gcgattttca tgctgttccc ctgccttatt tatcaagctc gccgtgtgaa
    12661 agcttcacaa gctcctccgg cgttatttca cacattttct gtgcattttc gctcttttga
    12721 atgtgcataa gatattcaat atttccctcc ggacctttga tcggtgaaaa atcaagattt
    12781 tttacgtcaa atccgttttc aagtgcaaaa ttcactatat tttcaacaac ctcaacatga
    12841 acctgcggat cccggacaac accctttttg ccgatttttt ctctgcctgc ttcaaactgc
    12901 ggctttataa ggcacacagc ctgtccgtca cttttgagaa gttcacgcag aacaggaaga
    12961 attattctca gcgaaatgaa agaaacatca acgcttgcaa agtcaatatc ctcgggaatt
    13021 tcattatgtg taacatacct gaaattggtg cgctccatgt tgacaacacg ctcgtccgtt
    13081 ctcagcttcc atgcaagctg accgtagccg acatcaatcg aatacacctt acttgcgccg
    13141 tgggtgagca tacagtccgt aaagcctccc gtcgatgcac ctatatccat acatatgcat
    13201 ccgccgagat caatatcaaa tttttccgct gctttttgta gcttcagtcc gcccctgctg
    13261 acaaaaggat tacggtttcc cctgacttca atttcatcat caggcttcac cgaagctcct
    13321 gctttaagct ccttccgatc attgaggtaa acctgtcccg ccataatcag cgcctttgcc
    13381 ttctccctcg attcggcaag tccgcgctga gtgagcgcta tatcaagtct tattttatca
    13441 gacaatattt acacctcagc cgtcttttca acgattttat tcaccatgct gtcactgtca
    13501 aatccgtact gcttaataag ctcaggtacc tttgcctgct taacaaagca atcctcgaca
    13561 gccgttatat cataattgcc cttgtaaccg tgttggagga gctcggcggc aaacttttca
    13621 cctattccgc cgcttctgac tccctcctca tagaagaaaa tatgcgttgc attcataacc
    13681 gccttgagcg cctcggggtc aatcggcttt atcctgttaa gctttaaaac ctttgcgtca
    13741 atactgtgat ttgccagctc ctttattgcc tttgcagctt caaaaaacag ccttccgtag
    13801 gttacgataa ccttatcggc agagacattg ccgtaaatat cataggttcc gaatgacggt
    13861 tcgtaatcct caggaatttc aggctcgcct ccccttggat aacgannnnn nnnnnaccgc
    13921 gacaacgtcc ttgtcacggt aaaatgcgtt accgaaatca atcttcattt catcataggt
    13981 cgccggggaa tatacagtta ccttgggaat actctgcaaa aatgcaacat cataaagtcc
    14041 gttatgcgtc tcaccgtcgt caccgacaaa gcctgcgcga tcaatagcga gcacaagctt
    14101 ctgatcctgc aatgcgccgt catgcattat ctcatcatag caacgctgta aaaacgttga
    14161 ataaaccgca aaaacaggca acattccgcc ctttgaaagt cccgatgcaa aagttacggc
    14221 gtgctcctcg gcgatgccga catcgaaaaa cctttcagaa tacttttcgc tgaactctgt
    14281 cagtcctgtt ccgagcgaca ttgcggcagt gatgagcgta atgcgtctat cctcatgtgc
    14341 catcttgcac atcagtttac cgaactcatt gcagaaagtg tctcccgacg aatgaggttc
    14401 gcccgtatct atatcgaaat ccgaaacacc gtgaaaatgg ctcggatcct tttccgcata
    14461 gttatatccc ttgcctttga cggtgttaac atgaagcaaa acaggtgcac cgagactctt
    14521 ggcagtctcc aaagcctcac acatcattcc aatatcatgt ccgtcaatcg gtcccatgta
    14581 tctgaatccc attttttcaa aaattgtgct ttcgtaaatg agatttttta tcgctgtttt
    14641 gaaactgaac agtccgcttg ccatgctgtg tccgataagc ggaattttgt caaggaaaca
    14701 ctcaagtctt gctttcattc gaacataacg tttctttgaa cggattttag taagatattt
    14761 tgcaacagag ccgacatttt tcgaaattga catctcatta tcgttgagaa taacgataag
    14821 atttgtcttt gacctgcctg cgttattgag tgcctcataa acaagtccgc ccgtaaaaga
    14881 accgtcaccg ataacagcaa ccgtataatt attgttcttt tttattttat tcgcttctgc
    14941 aatgccgaaa gccgaggaaa ccgacgttcc gctgtgtccg ctgtagaatg tgtcatgctc
    15001 gctctcattc ggatgacaaa atcccgatat tccgtccttt tggcgtatcg tatcaaagac
    15061 atcgtaccga cccgtcaaaa gcttatgcgt atatgcctgg tgacctacgt cccaaacgat
    15121 tctgtccttc ggcgagtcaa acgtcaggtg aattgcaatt gtcaattcaa ctgcgccaag
    15181 attggacgca agatgaccgc cgttttttga aacggtatca acaagtacat cccttatttc
    15241 ctgcgcaagc tcgggatatt cggagctgtt aagctttttc agatcagcag gcgaatgtat
    15301 tttattgaga tattttattt ccttatccaa tactcttcac atccgattaa acaggtaatt
    15361 agttctttct cttttccatt ttatcggcaa agtcaataag aaattcggca tcaccgtcaa
    15421 agcccttcat ggcttccttt gccttttccg taagctcgga aacaatctcc ttcgagcgtt
    15481 cgattccgag aagtgaaaca tatgtgcatt ttttgttttc atcatcactg cctataggct
    15541 tgccgagcgt cttttcgtca cttgtcacgt cgagaatatc atcgactatc tggaaagata
    15601 ttccgatgca tctcgcatac tcctcggcgg cggggatcat atccttccgt ccggcagcaa
    15661 tgcatcccat gacgcaggcg gcacgaataa gctcgcccgt tttgagcgca tcggtttttt
    15721 ctatatcttc aatgcctacc tttttgccct catttttaag atccatgacc tgaccgccga
    15781 ccattcctgc ccagcctgcc gcctttgaga gaacggcaat cgaatctatt attctgtcgg
    15841 caggcagctt cgctttaacc gccgtttcaa atgcaagcgt caaaagagca tcgcctgcaa
    15901 ggagagcata ttcatatccg aatgcaatgt gacaggacgg cttcccacgg cgaaaatcat
    15961 cgttatccat gcacggcaag tcgtcatgaa tcaacgaata ggtgtgtatc atttcaagcg
    16021 cacatgcaag cggcagtgca ttctctgcgt ctccgccgca agcctcacag aacgagagca
    16081 caagctccgg tctgatgcgc ttaccgccgt tctcaacgct gtagcgcatt gcacggctga
    16141 ctatatcctc gctctcgggc atatatccct ccagcgcctt gtttatcatt tcaatgtggc
    16201 tcatataatc attccttttc gtcagccctc ctgcttttcg gagagctcgg tgattttcag
    16261 ctttgccttt tcaaggctcg tgttgcaaaa tgaggcaagc tttgtgcttt cctcaaagag
    16321 ttttattgat tcgtcaagcg gcagactgcc gtcgtcaagc tttgtgacta tctcctcaag
    16381 ccttgccatt gcctgttcat atgtcatttt cgccgccatt taaagcacct cgcaattttt
    16441 ttctccgtcg gcgaatccga cggttattct gtcgcctttt ttaagctttt ttgagcttat
    16501 gacgaccttt tcatctttcg tgactatcga atatcccctt gacatgacct tcaacgggct
    16561 gagtgcgtca agccttgccg ccgttgcgaa aaatctcgaa ttttccttgt ccagcttccg
    16621 ctgtatgctc aaaagtgcgc tcgtcagata gttgtcaacg agctgcagct tttggtcaat
    16681 aagcataatt gggcttctgt attccagtct ttcttttata atatcaagct ttcttttttc
    16741 gtcgtttatt cgttgcaaaa gcattgcttc gcactcgtaa acgaccgttt gaacaaaggc
    16801 aatatcctcc cggatatccg gcacggcaag ctctgccgcc gccgacggtg ttggtgctcg
    16861 caaatcggca acaaaatcgc agatagtgaa atccgtttcg tgtccgaccg ccgaaataat
    16921 cggaatttta ctgttaaaaa ccgcccttgc gacgatttct tcgttgaacg cccacagatc
    16981 ctcaagtgaa ccgccgccgc gaccgacgat cagaacatcg gcatnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcccgaat taaattgctc aatagcttca
    17101 actatctgcg gagccgccga tgctccctgc acctgaacgg gagcaagaat aatctccgcc
    17161 agcggatatc gccttgaaat aacgtttctg atatcctgaa tgacagcgcc tgttgccgag
    17221 gtcacaacgc cgatccgtgt tgaacggtgc ggaatcggtt ttttataaca ctcgtcaaac
    17281 aagccctcct ccgccagctt atttttcaac tgctcaaacg caagattaag tgcgcccaaa
    17341 ccgtcaggct gcatatcatc aatatatagc tgatactgtc cgtcacgttc aaaaaccgaa
    17401 accctgcccc tggcaataac cttcataccg ttctccggca tgaatttcag ccgtctgttt
    17461 gccgaggaaa acataacagc cttaacggcg cagttctcgt ccttgagagt cagataaaaa
    17521 tgtcccgtcc tgtagtgatt cgtgaaattt gatatctcac ccacaacata aagacttctc
    17581 aggagcatat cgccgtcgat tatcgacttt atataggtat tcaattgcgt aacgctcagt
    17641 attgttgatt tcattcaatt gtcctcttgt gcgccagcaa tgcgattccc gcagcgttgt
    17701 ccgccgaaaa ttcaggggtt gcaaagcctg cgccgagagt tttttcaaac tcttctcata
    17761 taattttatt cgacataact ccgcccgaaa aaatcatcgg caaatcgccg tacttttcct
    17821 tcgcaaaaag tgcgatttta taaagagccg tcattatgta atcaatgcag tagcggcaga
    17881 catcctcggg cttttcgccc ttttcaaaca ttgccctgca tttattttca acgcccgaaa
    17941 gacagcagtt cccgtccttg agcttaatat ttatcttata ttttttgtcg cttttctgtg
    18001 ccagctcatc aacatatttt cccgccggga acgggtagcc gagcataacg ccaactctgt
    18061 caaccgcctg acccgctttc agatcgagtg atgaagcgat cagccttgca tccagctcac
    18121 cgctgacgag caatgcctgc gttgtgccgc ccgaaacatg aaaagcgaaa aaatccttgc
    18181 cgtacagctc acgcctgcct gatgaaaaca gagccgccat aatgtgtccc tgctgatgcg
    18241 aaaagcggta aagcggctta cctgttgccg ccgctatggt ctctgcggcg cacacaccgg
    18301 tcaagaagca aggcatatat gagccctccg ccctcatcgg tgcatcggaa acgcctattg
    18361 cgtcaatctc gccgtcaaag tcggcaaaaa gctccttcat cagttcgggc aattgaaccg
    18421 tgtgatgaaa aaccgcgtca ctctgacgga gtccgagctc accttttttt acaggcaaaa
    18481 gcttcttacg atgaatgata gtatcgtttt cgctgtcata aatcgcacat gaggttgtgt
    18541 aattgcttgt atcaattcct aaaaagcagg gcatttcaat cctctttttc tcttgaaatc
    18601 gagccgagaa ttccgtttac aaagccatta tcttcgtccg aaccgtagac cttgcaaatc
    18661 tcgacagctt cattgatcga aacaccgacg ggaaccttgt caaaatattt catctcgcag
    18721 acagccattc tcagcagtgc aaggctgacc tttgaaattc tctgcttgct ccagcccttg
    18781 agattactct caatggcggc atcgatctca tcaatatttt cctcagcctt tttcacgata
    18841 tcggcggtca tcttgtcctt agaaatgacc tccgtctcaa ttgcgatatc cattatttca
    18901 tcgaccgaaa gctccgtatt aaacgacttt tcaaaaagaa taataaaagc ctgctctctt
    18961 gatttacgct tgttcgcaga aggtgaaccc atttttacga ccaatccttt cgcttagata
    19021 taaaaagtcc tccgtgtatt atacacagag ggtcagtacg tctgttttca ttttgcaagg
    19081 tgcactgaat tgtatatata cactattcaa tgcatattat accacaatta aaaaaaatct
    19141 caactgtttt tttgaattat tatttaataa agtaacactt taacaggtgt ttttcataaa
    19201 ttacagtttt ccttcgggtt ttctcctcta taccggcaaa taaaccgcat aacttagttt
    19261 ctgtaatatc tgcaaaaatg ctgctgatta aatctatgtt taatggctca ctatttaatt
    19321 tcctactcaa gttcgtaata atcctcagca ttttatctat gcaatccgct tccacgaaaa
    19381 acgaccgcca cgagtgtgac ggtcgcaaat atttataata attatttctc gaggttagcc
    19441 ttgagagttg caagctgatc tctaagctcc tgcggaagct tgtcgccaaa cttcttgtaa
    19501 tgctcctcga tctcggctgc ttccttggtc catgtatcct tatcaacatc aagaattgac
    19561 ttgagtgtat caatatccat gtcaaggtct gtaaggtcga tatcctccgg cttcggaaca
    19621 taaccgatag cggtctctgt tgcgtcagcc ttaccctcgc agcggtcaac gatccagtta
    19681 agaacacgca tgttgtcgcc gaatcccggc caaatgaagt tgccctcgtc atcggtacgg
    19741 aaccagttaa cattgaagat cttcggagcc ttgtcgccaa gcttcttgcc catgtcgagc
    19801 caatgctgga aatagtcagc catatgatag ccgcagaacg gaagcatagc catcgggtca
    19861 cgacgtacaa cacctactgc accagctgct gctgcagttg tctcggaagc cattgcagaa
    19921 ccgatgaata caccgttttc ccagctcttt gactggtaaa ccagcggagc acactttgca
    19981 cggcgtccgc cgaagataat tgccgaaatc ggcacgccct cgcccttgtc aaactcagga
    20041 gagatgcacg ggcagttaac agccggagca gtgaatcttg agttcggatg agcggcatat
    20101 gtgctcttgt cagccttatt gaacttggaa gcatcccatt tgttgccctt ccagtcaata
    20161 gcgtttgtcg gaagatcctt attgagaccc tcccaccaaa cggtgttgtc atcaaggtta
    20221 agcgcaacat ttgtgaaaat tgtatttttc tttgttgact caagtgcgtt gaaattcgac
    20281 tttgcatttg tacccggtgc aacgccgaag aatccgttct cagggttaat cgcataaagt
    20341 cttccgtctg caccgattct catccatgag atatcgtcgc cgactgtcca gatcttgtag
    20401 cctttctcct gaagatactt cggaggaata agcatagcaa ggtttgtctt gccgcaggct
    20461 gacgggaatg cagcagcaac atatttaact tcacccttcg gattctgaag accgaggata
    20521 agcatatgc
//
LOCUS       DAWBSW010000011        20529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_570_length_20529_cov_2.723747, whole genome shotgun sequence.
ACCESSION   DAWBSW010000011 DAWBSW010000000
VERSION     DAWBSW010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 20529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20529
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_570_length_20529_cov_2.723747"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    387..396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7206..7215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12635..12644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15365..15374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16377..16386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5778 a         4359 c         4963 g         5379 t
ORIGIN      
        1 caccttgaat ttgttatccc tttccataat ctctgtcttg accttttgaa aatacttacc
       61 gatttcgggc ttaaagcgaa gcttatactc agaaagtatt catacgttgt ttatctaaag
      121 gacagcgaga gcattgagga tctgctgacc cttatgggtg cggttaattc aagccttgaa
      181 cttatgggaa ttaaaattca aaaggacgta agaaacaaga taaacagaca gatgaacttt
      241 gagtctgcca acatgagccg ctcaatcgaa gccggattgg cacaggttga agcgatagag
      301 attattgaga aaaagcaggg gcttgactcg ctgtcggacg agctgaagga ggttgcgatt
      361 ttacgcagag aaaaccccga tatgtcnnnn nnnnnntcac aggcttatga aaatcatgga
      421 aattgcggat aagtacaggt aagaagcaaa tataactttt ataataaagg aggcggcgcg
      481 ttgagcttca cacatcttca tttacatacg gaatacagct tgcttgacgg agcatgccgt
      541 cttgatgacc tgcttgaccg tgcggttgag ctgggcatgg attccattgc aattaccgac
      601 cacggcgtta tgtacggcgc tgtggatttt tacaaaaagg cgaaggcgag gggtatcaag
      661 ccaatcatcg gctgcgagtg ttaccttgcc tcaagaggcc gcaaggataa ggttcacgct
      721 ctcgacaatg agagatacca tcttgttctt ctctgtgaga atgcaacggg ttatcagaat
      781 cttatttcaa tggtttcaaa ggcgtggacg gagggctttt acacaaagcc gagaatcgac
      841 cgtgagcttt tggagcagta tcacgagggt ataatcgctc tttcggcttg ccttgcaggt
      901 gaaattccga gagcgctgat agctgatgat tatgagagag cgaaggaaaa ggctctttgg
      961 tacaacaaag ttttcggtca gggtaatttc tatcttgagc ttcagaatca cggaatccgc
     1021 gagcagaagc agattgagcc gatgctcata agactttcaa aggagaccgg tattccgctc
     1081 gttgcgacga atgatacgca ctatgtccgc aaggaggaca gcaagattca gcaggttctc
     1141 atctgcattg caaccaatac gaccctgggt cagggcaacg gtcttgaatt tgacagcgac
     1201 gagttctatt taaagagtga ggaggaaatg cacgagcttt tctcacatgt tcccgaggcg
     1261 atagaaaata cgcagattat cgccgatcgg tgcaattacg actttgaatt cggcaatacc
     1321 aagcttcctc attttgaagt gcccgacggc agagatcatt ttgaatggtt cagggagcag
     1381 tgctacgccg ggatgtacag aaactacggc gataatcctc cgaaagagta ttttgacagg
     1441 ctgaattacg agcttgatgt tattaacaaa atgggctatg ttgattattt cctgatagtc
     1501 catgatttta taagatatgc aaaatccaag ggaattcccg tcggtccggg cagaggttcg
     1561 ggcgcaggca gcattgccgc atattgcatc ggcataacgg gaatcgaccc gatgaaatat
     1621 aatcttctgt ttgaacgttt tctcaatccc gagcgtgtaa gtatgccgga ttttgacgtt
     1681 gacttctgct acgagcgcag gggagaggta atagactatg ttatcgaaaa gtacggcgca
     1741 gaccatgttg cacagatcgt taccttcgga acgcttgcgg caagggcggc tattcgtgat
     1801 gtcggtagag ctatgggaat accttataat atcgtcgata acgtttctaa gcaggttccc
     1861 agagagctga atattacaat tcaaaaggcg cttaaaaagt cgcccgaatt ccgttcactg
     1921 tatgaaagca gcgacgagat aaagagtctg attgatacat ctatgaaggt tgagggtatg
     1981 ccgagacata cctcgaccca tgcggcgggt gttgttataa caagagaccc cgtggcgagc
     2041 tatgtgccgc ttgcgctcaa cgataattcg cccgtcactc agtatacgat gacgacgctt
     2101 gaggagctgg gactgctcaa gatggacttc ctcgggctga ggacgctgac cgttataaac
     2161 gatgcggtca agatgataag acttaaagac aatagctttg atatcaacag gatagacata
     2221 gcagacaagg caacctatga gatgatggct tcgggtcaga ccgagggcgt gttccagttt
     2281 gaatcggcag gcatgaaaag cgtgctcata gggctgaaac ctgtcgatat tgaggatttg
     2341 atagctgtta tctcgctcta ccgtccgggt ccgatggatt cgataaatac ttatatcgaa
     2401 aacaggcatc atccggaaaa gacagtttac aagacggaaa agctccgtga gattcttgaa
     2461 gtcacctacg gctgtatgat ctatcaggag caggttatgc agatattccg ctctcttgcc
     2521 ggatattcct acggcagagc cgatattgtc cgccgtgcaa tgtccaagaa aaaacacgat
     2581 gtcatggaaa aggagcgtaa aaacttcatc tacggtattg tgcgtgagga cggaacggtt
     2641 gaatgtgacg gctgcgttaa aaggggaatt cccgccgagg ttgccaacga tatattcgat
     2701 gatatgtcat cgtttgcctc atatgcattc aacaaatcac acgcaacggc atacgcatat
     2761 gtcgcatacc agacagccta tcttaaatgc cattatcctt gcgagtttat ggcggcgctt
     2821 ctttcaagcg ttcttgacaa tacggacaag gtaacggaat atactgatga atgtaacagg
     2881 ataggcatta aaatcctgcc gccgaacgtc aatgagagca atgacaattt caccgtttca
     2941 aacggaaata tccgtttcgg tatgcttgca attaaaaatc tcggcaggag ctttatcgga
     3001 aatataattt ctgaaagaaa cgcaggcaaa ttttcatcct tttataattt ttgctcgaga
     3061 atgcacggct cggattttaa taaaagagca gttgaaagtc tcattaaaag cggtgcactt
     3121 gacggcttgg gagcaaaccg ccgtcagatg ctcatggcga tgaatgagat aatcgacgag
     3181 cttgactcaa agaaacgcag aaatgttgag ggtcagatcg gtctgtttga ttcggtcggc
     3241 ggcgaatctc actcggaatc ggcattgaaa ttctttgacg aatttccgca aagtgagctt
     3301 ttagcaatgg aaaaatcgac aactggctta tatctatcgg gtcatccgat ggcatctcat
     3361 gccgagcttg cggcttcaat gggttgtgtg agaattgccg agcttgcaaa cagcggcgaa
     3421 aatatacatt cttcgcataa ggacggagaa agaaccaaga taatgggtat aataaccgag
     3481 gtgaagaaaa aaatcacaaa gagcgacaca acgatggcat ttgtcgacgt tgaggatacc
     3541 tctggatcaa ttgagattat tgtattcccg aaagttttaa cagaaaatgc aatgatgctt
     3601 gagacgggca gggttcttgt cttttacgga cggctcgatg tcagggacga agaaccgtca
     3661 aagcttattt gtgagcatat ttatacgctt gaagcggcaa aggattattt tttgcaaaag
     3721 agtgttcagg gctcggattc aattgccgga cctgtcagga aaaggcgcag cggactgttc
     3781 ctgaaattcc cgacggagcg cactccggaa caggttcagg cggaaaagct ccttgcaatt
     3841 ttcgacggca gagttccgct ttattattat tttgccgata ccggaaaata cgttcatcag
     3901 ccgtatgaac gcagtgttga cgtcaacgaa cctttgataa acgagcttaa acggcttctc
     3961 ggcgaagaaa atgtggtttt tcagtagttt tcggcaaata tttttctgtt ttatttgaca
     4021 ttttttttat ttaggtgtat catcatatag tatttttaga gaatccttag gaggaagtta
     4081 taatgaagac aatcggagtt ttgacaagcg gcggagacgc cccgggaatg aatgctgccg
     4141 ttcgtgcagt agttcgtaca gcgtgtgaga accatattaa ggtttacggc atcgaccgtg
     4201 gctataaggg ccttatggag ggctctctca tcccgatgga gatccgcact gtttgcgata
     4261 ttatccacag aggcggcaca atgcttcatt ccgcaagaag caaggagttt gcaaccgagg
     4321 aaggtatgca gaaggctatt gaaatctgcc acgcatacgg cattgaaggc attgttgtta
     4381 tcggcggtga tggttcattc agaggtgcaa gagacctttc cgagcgagga attccctgcg
     4441 taggtattcc cggcacaatc gataacgata ttgcttgctg cgactataca ataggctatg
     4501 acacttgtct taacacaatt atggaaatgg ttgacagaat ccgtgacaca tcggcttccc
     4561 atgaccgctg ctctgttatc gaggttatgg gcagacgcgc aggatatctt gcccttaatg
     4621 caggtatcgc agtcggcgca acggctatcc ttatcccaga gattgagttt gacctcaaga
     4681 gggacgtatt cgacagaatc cgccgtactc aaaagacagg taagaagcat ttccttgtta
     4741 tcgttgcaga gggcgtaacg gctgacggaa acatcaccgt tgatgagctt gcaaaggcta
     4801 tcgaggaaga aacaggcgtt gagtcaagag caatggttct cgctcatgtt cagcgaggcg
     4861 gctcaccgac agtaagagac cgcgttgttg caagccagat gggctacaga gcggttgagc
     4921 ttctcatgga cggcatcggc aaccgtgttg ttgcaatgca gaaggatcag atcgttgatt
     4981 atgatatctt cgaggctctc aacatgaaga agcatattga catgaatctt tacaagatgg
     5041 caaacataat ttcaatctaa aggaaatcaa attatgctta aagacgaatt tttagaactt
     5101 cgccgccgta taattgcaaa agattttgaa agaatgaatg acaggcagca agaggctatc
     5161 tatgcagtaa atggcccctt gctgcttctt gcaggtgcag gcagcggaaa aaccacggtt
     5221 cttgttaaca gaattgccaa ccttatcaag tacggcaacg cttataattc ggactattcc
     5281 gaatttgaac cgagcgagaa cgatattgcc gttatgaagc attaccttga cgatccgacg
     5341 acggatctgt tcgaggtcta tgaccttttg acggttaatg ctcctgctcc gtgggagata
     5401 cttgccatta ctttcacaaa taaggcggcc tcggagctca agggcagact tgccgcaaag
     5461 ctcggcgacg ccgcaaacga tatatgggcg agtacttttc attcctgctg tgcaagaatt
     5521 ctcagacgtt acggtgattt aatcggctac acgaatcgct tcacgattta tgacacggac
     5581 gattcaaagc gagttatgaa ggaggcacaa agactccttg atatcaacga caagatgctt
     5641 ccgcacaaga ctattctcaa agagataagc cgtgccaagg atcagctcat aacgccggac
     5701 gagtacattg cccgggcggg cggagatgtt cgcctaaagg cgataggtga ggcgtacaag
     5761 tgctatcaaa agcttcttaa aaatgccgac gcaatggatt tcgacgatat gattttcaga
     5821 accgtcgagc ttttgcaaaa gaacgaagag gtcagaaatt attatcagaa taaattcaaa
     5881 tacatactgg ttgacgagta tcaggatacc aaccatgcgc agtatgtgct gacaagcctg
     5941 cttgccgaaa aatacagaaa tatttgcgtt gtcggcgatg atgaccagag tatatataaa
     6001 ttccgcggag ctacgattca gaacattctg agctttgagg agcgctatcc cgacgcaaag
     6061 acgataagac ttgaacaaaa ctaccgttcg actcagacta tccttgacgc ggcaaacgcc
     6121 gttattgcca acaacagtca gcgcaagggc aaaaaccttt ggacggctaa cggcaagggc
     6181 aacaagatta acgtcaacac ctcatatgac gagagagagg aagcacgctt tgttgccgac
     6241 acgataatga aggacgttgc gtcaggcaga aaaatgtccg accatgccgt actttacaga
     6301 atgaatgcac agtcgagcac gattgaaaat gcgtttgtcc gtgcaggtat tccgtacagg
     6361 atcatcggcg gcctgcgctt ctatgagcgt aaggaaatca aagacgcttt ggcatacctt
     6421 acacttatca acaacacggc ggacaatgtc cggcttcgcc gtataataaa tgaacccaag
     6481 aggggaatag gcgatacaac gctcaacaat gcgtcggaaa ttgccgcagg actgggcgtc
     6541 agtatgtttg aggtcatttc aaatgccgac tcctatccgt ctctcagccg cgcagcttcg
     6601 aagcttttgc agttcagtgc aatgattaag gagcttatag aagcggcaca ggagctgaag
     6661 cctcacgagc tgcttgaaaa aacattaagc atgacaggct attatcagtt ccttgacctc
     6721 gataaggaca cggcgcagga cagaaaagag aacctcaacg agcttaaagc taacctcatg
     6781 cgttatgagg aggagagcga cgagcctacg ctcaacggct ttcttgagga aattgccctc
     6841 atgaccgata tcgacaacta caatgccgat tccgatacgg tagttatgat gacgcttcac
     6901 tcggcaaagg gacttgaatt ccccgttgtt tttataatcg gcatggagga cggagttttc
     6961 ccgggaaatc agtcgatcta taatcccgac gatatggagg aggaacgccg ccttgcatat
     7021 gtcggtataa caagagcaaa gcaagaactc tacctgacaa acacaagaac acgccttatg
     7081 ttcggttcaa cgacccacaa tgcaccgtcg cgctttctcg gtgagattcc ggacgatttg
     7141 acggaaaaga cgggtgtaac ctcgtattct tcatacagaa gaagtgcgga ggacgagcct
     7201 tacagnnnnn nnnnncttca cggctcagac aacggcgaga aaaccacagc ctgccgccgc
     7261 aagcagggaa acctacaaaa tcggcgatac agtcacacac aaggtgtttg gacagggtgt
     7321 tatactttcg gccaagagca tgggcaatga tacacttttg gaaattgcct ttgaaaaatc
     7381 gggcacaaag aagctcatgg caaattttgc taagcttaca aaataataaa atttaatcaa
     7441 aacttaagat ttgaagtatt gatttattct gtgactgatg atataatctc aatgaagaaa
     7501 ataagtttga ggtgattatc atgacaaaaa cagcaaacaa atttttagcc gtttttcttg
     7561 cgattgcaat gattttcagc ttttccgtta tcggctttgc cgaggaggga aagtttaatc
     7621 tgaaatccgg agaagcggtt gccgcatcgg gaaactttaa agacaaatat gtcggtgatc
     7681 tcaaaattac ggttaagtcg aacagtgagt ttgtgcttgc ggatggcttt tcggtaaatg
     7741 tgagcggaac agacggacag aataaaacat acaatgcttc aaatgcaaag aatattgaaa
     7801 tgaagggcaa gagtgcggtt attaccgttt catatgagtc gggaatgact catgaggcgg
     7861 agtacaagat cacttttgca gagggctcat tcaagacggc tgacggaaag ataagctcga
     7921 attattcgtt cacgacaacg ggaaatctca ttctcgaaaa gctgaacggt gaccgtccga
     7981 caactacgat gcagaagttt atcagatggc tttcgggctg gaagtatgca gatatcataa
     8041 agccgataat tgatcttctc aagtggtttg attcacttta atatcaacga tttaagcctc
     8101 tccttgattt ttaaggggag gcaaatttaa tttttaggca tcggataatt tatatgcaaa
     8161 aaataaaaga tatgaaaaac attgattacg cgatttttga catggacggc acactgcttg
     8221 attccatgca catatgggat acagcctcgg gcgctttcct catgatgagg ggcattatcc
     8281 cgagggaatt tgaccttttc cgcaagcagg gctataaaac gggaataagc tatatgatta
     8341 aggagtacaa cctcgatatg agctttgacg aagtgctcga aagcttgcag gagattctgt
     8401 ggttctacta ttcaaacatt gctcctatca aggacggagt aaaggatttt ttgcagtcgc
     8461 tcaaggataa cggcgtcaaa atggtcgttg caactgcgac tgagagcaga atggcaaaaa
     8521 aggcgcttga gcgcaacggc atactcggct attttgagga tgttatctca atgcatgaga
     8581 taggtattca taagaatgac cctgctgcat ttgaatatgt caaaaatgcc attcatgccg
     8641 agggtgtcgg ctatgtattt gaggacgctc tctacgcaat agaaaccgcc aaatcggcag
     8701 gctataaggt gatcggcatt gaggattatt ccaatgagga cgacagggac gagataaaga
     8761 aaatcgtcga tttctatgcc gaaaattacg aacaggtgaa gaaatatttt gaattttaac
     8821 tacggggaat aaaaatgaac atctatcctt tctttaagaa ttgtattacc gtcggtctgc
     8881 cgaaagagga gattatcggt atctttgaaa aaaatgatat aaaaacggac accatggctt
     8941 ataatttcaa gtttgataaa gagaaaagct attacattcc gtataaaaat ccatacggtt
     9001 ggggagtacg tcaatcgttt gttcccgaaa tatatattgc tctttcggag cagggcacag
     9061 aaacgaaaat cacattgcat ttttgccttg aaagaatgtg cgaagaggtt attacccttg
     9121 tgtttgcgat cgttttgacg ttttctattt tcttatttgc tacaggcttt ataaaagaat
     9181 ttatacccat aatgcgccca atcccgttga taatgctcct gctaacatat tttatgccgt
     9241 tgttttatat aggcaagtat cgttcaagag cagtaaaaga gatacaaaaa atggtcggga
     9301 aagaaaagtc tgaattgtat caagattagc agaaacatta ttcggctata cgacaacgaa
     9361 aattatacaa aataattcaa taaaaccctt gacattatcg gcattagctg ataaaatatt
     9421 aaggctgtaa aatattgatt acagctatcc ttgccgctga ataggcggcc ggaatgacca
     9481 aaaggaggtg cttttaaatg gcagtagaga actatgaggc aatgatcgtt tactccgtta
     9541 aggagagcga ggatgcagct aaggctttgg ttgaaaagtt caaggctctt atggagaagc
     9601 atggtactgt tgaaagcgta gatgagtggg gtaagcgcaa gcttgcttat gctatcaact
     9661 acgagacaga aggctactat gctctttaca atttctcggc tgagccggaa ttcccggcag
     9721 agcttgaccg tgtccttaac atcacggacg gcgtgcttcg ttcacttata atcaagaaat
     9781 aagggggacg caacatgatt aacaatgtag tattgatggg tagacttgtt gcaactcccg
     9841 agttgcgcaa tacccagaca ggtcttgccg taacgtcttt ttcgcttgct gttgagcgtt
     9901 catatgctcg tcagggcgaa cagcgtcaga cagatttcat caattgtgtc gcatggagaa
     9961 ataccgctga atttatctca agatatttcc agaagggctc tatgatcgct gtaacgggtt
    10021 cgattcagac ccgtaactat gaggacaaaa acggtaataa gcgcacagca gttgaggttc
    10081 ttgttgacca ggcaagcttc tgcggctcaa aggcagaaac gggcacaggc aacaacaatt
    10141 acaaccagag ctattcggct cctgcgcagc ctgccgctcc ttcattctca accggttccg
    10201 acggtgactt tgaggagatt tcaggagatg acgatctgcc gttctgagca gatgtcaccc
    10261 gaattttata aggaggtatt ttgtaatggc ttatgataag aacaacggcc gtcctaacag
    10321 aaaaggccgc aagaaggttt gttccttctg tgttgataaa gttgagagca ttgattacaa
    10381 ggatgtaaac aagctcaata agtacacttc cgacagagcg aagattcttc cccgtcgtgt
    10441 aacaggtact tgtgcaaagc atcagagaga gcttacaaca gctattaaac gtgcccgtca
    10501 cgtagctctt atgccctaca cttcagactg atttaacagt tatcggactg tcgcttttgc
    10561 ggcagtcttt ttttataatc aaaatcgtcc ggtcagaccg gagacattct gaaatgaata
    10621 ttgataaaat aacgtataaa tgatataata gcccaaatga taaaagaggt ggtattgtga
    10681 aattcaaaaa tgttctgctt gcaagtgact ttgacgggac acttgccgac tgcaacggca
    10741 aaattccgga aagtgtgatt tcggcgataa agtattttat gtccgaggga ggcagattta
    10801 ccgtttgcac gggcaggatt tttcaaggct tccatcttta cagcgaggag tatatcaacg
    10861 ctcccgtgct tctcggcaac ggagctatgg catatgatta taaaaataaa gagattgttt
    10921 tcaatgatgc tataggagac gagggaatag cttcgttaga ggatattatt ttacattttc
    10981 cttccgtggc ggtcgaaact tatagcttca acaaggtttg cagcataaat gcaggcgaga
    11041 acagcataca tcatttcaca agcatgggaa ttgatttcac agaggttgaa tctcccgaaa
    11101 aggctcaaat tccgtggacc aaggtcatga tttacgctca cggctgctcc ttagaggtgc
    11161 agaagctcct tgacagacac gcagaggtta actatctgaa aacgacgggc gaatacatag
    11221 aaatcctcaa aaggggcgtt gacaaaggta caggcctttt aaagctcgga gaaatgcttg
    11281 gctgtgaacc ttgcgatatc tatgccgcag gtgacggcta taatgatgta gatatgctta
    11341 gagctgcagc aaagggcttt gtaccggaga acggttcaca ggaggcattg gcggtagccg
    11401 attatgtaac gagaacgaat aacgacggct gtatcgcaca tgcaattgaa attcttgata
    11461 agctttatta aagattaaga ggctttatag aaatataaaa tagcggcatc ttttaataat
    11521 aagaataaag tataattacc ccttctgcgg caataaaccg tgggaggggt aatttatatt
    11581 cggatcatta tttatctatc acgggcggaa tgaaattctc aattgccttt ctgagcttct
    11641 gatggcgaat tacaaaatga atggcaggtg ccttgctttt tgaaaccatg acccactgat
    11701 cctcgtgaat taaaatctgc aaatttctga aagcagggat agaggctttg agcagcttat
    11761 agtttgaatg gcttgcggca aattcttcgg tcatagcaag gtgagacatg tattcctcat
    11821 agctataggt gatgtccgtt tcgcagaata tgccggagag ctccaaaacc tgcggatgag
    11881 cggcaaactc gtcctttgtg acagtcggaa tttcatcctc aagaattttt gtttcgagta
    11941 ttttgatcac tcgtagcttc tgtgcttcgg catattcaag tatatccttt tgccgctctt
    12001 cggaaatttt atttcgttta agtatttgtt tcagcagctc acggtcggcg gtgtacagcg
    12061 gaagtgttga taatatgctt ctgcgcttgc cgggtttgtc cgagtcggag agcagaaatg
    12121 cgttaagctc attttcttta tcgcttcggt atatttccat gagcggataa gcgtttgcga
    12181 caagctcctg tgcacgcttt tcatagtatg cgatttcttt tcttgatttt gtcagataat
    12241 attttccgtc actgtggtat cctgcaatag cttcgcccgt gagcgcggca gagcctgata
    12301 ctttaagaaa atggtggaaa acaccgttct gtatgccctt taaataatag ggtgagatct
    12361 gaccggtcat atacatcgga atccagcttt ccaagccgag catcatctca tcaaaagagc
    12421 gatctatgtt gtgaatttga ttgaggtgaa gacccttttt cagcatcatt gccatgccga
    12481 acatccattt cttagagaaa tccgcgtcct ttgccatttc ttttaagggc atatcgctgt
    12541 acattgttac cggcgacatt gattttgaga gaactgtcgc tttaagaaaa tcaagctcac
    12601 tttctttcat ttcttctatg ccgtagtatg ttctnnnnnn nnnnaaccgt cggcaccttc
    12661 agctcgtcaa atttaatgac cttgatgtat tcgttaaggt taaattcatc gagcctgttc
    12721 aaaaagactc cgatggtgtc gggtttctgc tgcttttctt tgacaagcca tttctttacc
    12781 gttgtatata ccgcagactg gtcggttatt tcatttgtac tgcagccgag cagctctgca
    12841 aggacagtca gctcggaagg cgatttcatt tcttcggcaa taaaggaagc cacggcagat
    12901 gaaaagcctt caaaatcggc cgggtttctt gttccgtttc tgatacggaa aacggtcgaa
    12961 acatcatagt ttatgtatct gcacatacgg tttatgttaa tactaagcgc agaaataaga
    13021 gtattgaagt ttttgcggag aagctctttg tcggtcgaat caaagtcgga gcatgaacag
    13081 aattcctcct tgattttctc atatgtaaga ttaggggctt tgctttcggc gatttgggct
    13141 attccggtgc aaagtccgtc aaagcctttg gttccaagct cgggaacacg ctctccgctt
    13201 ctgtaacggc tgagcgttgc ttcggaaatt cccgaaaggg cacaaagctc cttagccgtg
    13261 caggatatcg aggcaatata ttcgtttagt ttttcacaga atttcatttg aaatacctca
    13321 taaaagatga tttatcgaca ttatacaaca ttttattcgg aattaaaagc cttgttaacc
    13381 ggataatttc agaattggca tttgactatt cggaaatgcg gcttaattat aacccgtgaa
    13441 catgctataa tcatcgcaag ggtatagcag aagagttatg attttaaaat aattttaaaa
    13501 acggaggttt tgtaaatgtc ggaatcaaga gtatttatgc tgaacggcac ggaggtgcag
    13561 gctgttgttg acagacttga gacctttttg cataccgaaa agggaatgga ggttcaaagc
    13621 tcacagacgg cagacggata cgtgatgcag gcaagtcagc cgaaggacgg ctggaaaacc
    13681 ctgacgggaa tgagactcgc cgttacaatc cagttgacgg tttttgacag tcagttgaat
    13741 gtaaccgtcg gtgaaggaca gtgggcggat aagatcggag cgggtgcttt gggcttgttc
    13801 atcgcatggc ccttggcaat taccgccgga atgggtgcat ttaagcagaa aaaacttccc
    13861 gatgagattt tcagaacggt tgaaaactgc attatttcag gcggacgttc tgccgtttac
    13921 ggcggagcgg aacattcggc acacactgca gccgaaacaa tggcgtgccc gaaatgcggc
    13981 gcacaactgc ctgtcggttc aaagttctgc aaccaatgcg gagagaagct cggaaatgta
    14041 tgcccgagct gcggtgccga agtcgttcaa gggagtatgt tctgctcaaa ctgcggacaa
    14101 aagctacaat aaaataaaaa tgtttatatg cgaacgggtt gacatgtttt gtcagcccgt
    14161 ttgcaattat ataaatgccc gtgtctcaac aaaccatttg ccgtcatcaa caaaaaagtc
    14221 acgggtctga ccctgaataa tcagggtata ttttatagct ctgccgcccg aacggacagg
    14281 ggaattgttc ttgatgttta taatctgctc aacagtgaat tttctgccgt ccttccagag
    14341 tatgctcagc ggcacgacct gcccttcggg aagcacttct atatacattg tcacatattc
    14401 tcggttgtat ttgacataca taatctgacc tcccgattta ttatctgaac aaatgttcgt
    14461 gtatattata actcattttc gagacatgtc aagaggggat ttttgcatgg aagccggcag
    14521 gagcggattt tgcctcaaat gcaggggaac gctcaaagct tcggtaggga taaagaggag
    14581 cctgtataat ttttaaatga tgttattttt actcaatcaa tccgtgatat ctcagcgtgt
    14641 aatcaatcct ttcctttgcc gagtcgctcg gacgggtgag aggaaggcgg caaactccgc
    14701 tgtctgtgcc catttgtgcc attgcatatt tgaccggcat cgggtttacg tcgcagaaaa
    14761 gcacgttaca caagccgaga taccgaagct ggagagcggt tgcataatct gcatctccgt
    14821 cgagtgccgc cgacactata tcatgggacg cctgcggcat tatgttagag agaacggaaa
    14881 ttgttccttt tcctccgagc agggtaaagg cagcgctctg gtcgtcgttg ccgcagtaga
    14941 tatccaattc gtcaccgcaa agcataagtg ttttcatcag tgcggaaagg tctccgttgg
    15001 cttcttttgt tgccacaaca ttttcaattt ggctgagtcg tttgtaggtt tcgggtttta
    15061 tgttcactcc cgttcgtgac ggcacattat aaacgattat cggctttgtt acgctgtcgc
    15121 ttatgcgtga atagtgctct accaatccct cctgcgagca tttgttatag taaggcgtaa
    15181 ctatcagcag tgcatcaacg ccgagcgatt ccgccgattg acttttcttt ataacgtcct
    15241 gtgtgttgtt gccgcccgtg cctgcgataa cgggaacctt gccgtttacc tgcttgaccg
    15301 ctgttttgat tacctgacgt cgttcccttg agtcaagggt ggcgctttca cccgttgtac
    15361 cgcannnnnn nnnncggcgt gactatggca catgccgcac ccgtaaaaac tgtttgtttc
    15421 attaaaacgc tccttatata aaaataaaga caggaaaatg aattcctgtc tttttaacga
    15481 tatgatgtgt ttatcagtcc atatatccct ttgcacagag aagctctgca aggagaactg
    15541 cgccgccggc ggcaccgcgg agggtgttgt gtgaaaggca cacgaactta tagtcgtact
    15601 gagtgtcctc acggagtctg ccgatgctga ctgccatgcc gccctcgagg tcacgctgaa
    15661 actttgtctg cggcatattg tcctctgtga aatagtggag gaactgcttc ggtgcgctgg
    15721 gaagtgcaag cttctgcggc tcgcccgaga agttttccca caccttgaga atttcttcct
    15781 ttgacggagc cttgtctgcg aatctcatga atactgcacc catatgaccg tttgaaacgg
    15841 gaacacggat acactgagct gtgaagtgcg gctcggatgc acaaacgatt tcaccgtttt
    15901 caaccttacc ccaaatttta agcggctcct gctcactctt ctcttcctca ccgccgatga
    15961 acggaataac gttatctacc atctcgggcc atctctcaaa ggtcttgccg gcgccggaaa
    16021 ttgcctgata tgtgcaaacg agaacgtcct caaccttgta gccggccttg tcaagcggat
    16081 aaatcgccgg aacatagctc tgaagcgagc agtttgactt aacggcgatg aagcctctct
    16141 ttgtgccgag acgctttctc tgatccgcaa taacctctat gtgatcggcg ttaagctcgg
    16201 gaacaaccat cggaacatcg ggagtaaatc tgtgagcgct gttgtttgaa acaacggggc
    16261 actctgcctt tgcgtatctc tcctcaagag ccttgatctc gtcctttttc atgtcaacgg
    16321 cgcagaaaac gaaatcaacg agcgaagcga ttttctcaac atcagccgtt gcgtcgnnnn
    16381 nnnnnngtaa acgacaaggt cagccatgct ttcaggcaac gggttgtcca tgcaccagcg
    16441 gctgccgaga gcctctttgt atgttttgcc cgccgaacgg ggacttgcag caacaagaac
    16501 aacctcgaac caagggtgat tttcaagcag ggtcgcaaat ctctgtccaa ccatgccggt
    16561 agctccgata atacctacct tgtacttttt cattacaaat tcacctctta aataaatttt
    16621 taaaaatgtg ttgccattcg accgattatg caatataata attagttgtc aacactttgt
    16681 aaccgccggt ttgatctgcg gttactgtta gaagtgatga taccattttt tattattcaa
    16741 gtcaagagcc aaatggccga aacagaggtt ttacgatgaa aaaaagtgat ttttattacg
    16801 atcttcccga ggagctgata gctcagcacc cgattgagcc gagagacagc tcaaggctta
    16861 tgcatctcga taaaaaaacg ggcgaggtcg agcatcttca tttttacgat atagtcgatc
    16921 ttcttcatga gggtgactgc cttatactaa atgatacgag ggttctccct gcacgtcttt
    16981 acggcgttaa ggaggagacg ggtgccgttg ttgaatttct cctgctcaat cagcatgacg
    17041 ctcacacatg ggaggtcatg gcaggacccg gcaagaaggc gaaagaggga gcaaaattca
    17101 ctttcggaga gggcatactc aaagccgaga taaaggaagt gcttgaaaac ggaaacagaa
    17161 ttgccgagtt ctcgtatgag ggcaatttct ataacgtcct cgacaaaatc ggtgaaatgc
    17221 cgcttccgca ttatataaaa gaaaagcttg aaaacaagga acgatatcag acggtctatt
    17281 caaaggagct tggttcggca gccgcaccga cggcgggctt gcatttcaca cccgagcttt
    17341 tgcggaagat caaggataag ggtgtaaaaa taggctttgt aacgctccat gtcggcattg
    17401 gcaccttccg tcccgttaag acggaaaata tcgaggagca caaaatgcac tccgagcact
    17461 atatgattcc tgccgaaacg gcggatatta taaacgagac gaagaagaac ggaggcaggg
    17521 tcatcgcagt cggcacgacc tgctgtcgca cgcttgaatc cgtatgtcag gcgaagggca
    17581 aggtttgcga atatgacgat tggacaagca tttttattta ccccggctat gaatttaagt
    17641 gcattgacgg tcttatcacg aactttcacc tgcccgaaag cacgcttatc atgctcgtca
    17701 gtgcattttg cggctatgaa cacacgatga acgcatataa aattgccgtt gaagaaaaat
    17761 acagattttt ctcgtttggc gatgcaatgg ctattcttta aggagacgga aaatggcaga
    17821 attcaaaatt ataaagcagt gcggagcggc gcgtctcggt gaattcaaaa cggttcacgg
    17881 aacggtcaaa acgcctgcct ttcagaatgt cgcaacctgc ggagcgataa agggtgcgct
    17941 tgacgcatat gacctcaagg acattcatgc ccaggttcag ctttgcaaca cctatcatct
    18001 ccatgttcgc ccgggtgatg agcttattaa ggagcttggt ggactccacc ggttcacggg
    18061 ctgggacggt cctatcctga ccgacagcgg cggatttcag gttttctcgc ttgcaaagct
    18121 ccgtcagatc aaggaggagg gcgtaacttt tgcctcccac atcgacggca gacgtatttt
    18181 tatgggaccc gaagaaagca tgaggattca gtcgaatctc gcttcaacaa ttgcaatggc
    18241 ttttgacgag tgcgttgaaa atccctcgac ctacgaatat gccaaaaagt cctgcgaaag
    18301 gactacaaga tggcttgtaa gatgcaagga ggagatggaa agacttaatt ccctgcacga
    18361 cactataaat aaaaatcagc ttcttttcgg catcaatcag ggctgtgttt acgatgagct
    18421 gagaatcgag catatgcaga gaattgccga gcttgatctt gacggctatg caatcggcgg
    18481 acttgccgtc ggcgagccga aggaggatat gtacaggatc atttccgccg ttgagccgta
    18541 tgcgccgaaa aacaagatcc gttatcttat gggtgtaggc actcccggca acatcattga
    18601 ggctgtcagc aggggagttg accttttcga ctgtgttatg ccgtcgagaa acgcacgcca
    18661 cggacatctt ttcacaatga acggaataat caatatcaac aatgccaagt atatgaagga
    18721 cgatacaccg attgaccccg agtgtgactg cccgacctgc cgcagacatt ccaaggctta
    18781 tatcagacat cttttcaagg ctaaggaaat gcttgcgatg cgcctttgcg ttactcacaa
    18841 tctttatttc tataatacgc tcatggagaa aatacgtgac gctttggata acggaacatt
    18901 tgaggaattc aaggaaaagt acgttgattt gctcgatacg agaatttaat ttttaaaatt
    18961 aacagaatat acacaaataa cgcttgcaaa aacttacatt ctaatgtata atacaagcgt
    19021 tatgtataga ttaggaggat aaccaatgaa ctatttaact ttattagcag ctcaggcaac
    19081 cggaaaaggc agcagctgga caatgattct catgatggta gctgttttcg ccgttatgta
    19141 ttttcttatg atcagacctc agaagaaaaa gcagaaggaa gaacaggcga tgcgtgacaa
    19201 ccttcagatc ggcgacgaga ttactacgat cggcggtatc gtcggcaaga tcgttaccgt
    19261 taaggacgat tctctcatta ttgagacagg cgcagacaga aaccgtatga agatcacacg
    19321 ctgggctatc agccagaaca acacggctaa cgagaagctt gcggcagagc gtgcagcagc
    19381 caaggaggca gccgagaagg aaaaggcagc ccgtatggaa gctaagggca agaccccgaa
    19441 gaaaaagaat aaagaagaga aattctaatc gaatttaagc tttatctcaa aaatcccgac
    19501 tctaaggagc cgggattttt ttgatggtat gaagataatt aataattcag aggagcaatg
    19561 cttctgtcca ggattccgtt catctgggcg attgcgacgc cgtaattgac tatcttgacg
    19621 ccctcttgaa cggcatgagc gattctgtat ttcatctccg tttcgttgag catacagccg
    19681 ccgcagtgaa cgacgagggc atatttgctc agatcgtcgg gaaattcggt gccggaggtg
    19741 aattcgaaat tgagcttttt gccggagtat ttcctaatcc agttcggaag cttgaccgtg
    19801 ccgatatcgt tgcactgcct gtggtgcgta catccttcgg aaatcagaac cgtgtcgccg
    19861 tcctccagct ttttgagctg tcttgcgccg tcaatgagct gcttcaaatc gcctttatag
    19921 cgtgcgaaaa gaatcgagaa tgaggtaagt ccgatatcct ccggaacgat tgcgaaaacc
    19981 tgttcaaaag cctgcgaatc tgtgataacg agcttcggcc ttttgacgag atttttaata
    20041 gtagcgtcaa gctccgtcac ctgacagcag actgccgtac agttgttgtc aagaagctct
    20101 ctgagcacct gctgctgagg aagaatgact ctgcccttgg gcgcggactc gtcaatcggg
    20161 ataacgagaa tgaccgtgtc gcccttttcg agaacgtcgg caacaatgtc cttgccgatc
    20221 tgcttcatct tgcagaatga agcgattttc tcttttaatt cataaacgtt tttgtcgttc
    20281 tttgcgctga cataaatctc atttttcgcc gcatccggca catttttaag taaatccgat
    20341 ttattgtaag cgataacaaa cggaattttc ttgtccttga agaagtctat catcatgttg
    20401 tcgaatttgc cgagaccctt agttgaatcg acaaccaaaa cggcaatatc tgtctttgta
    20461 agcacctgcc ttgccttttg aactcgcttt tcgcccagct caccctcatc gtcaaggccc
    20521 ggtgtgtcg
//
LOCUS       DAWBSW010000012        19886 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_600_length_19886_cov_2.661137, whole genome shotgun sequence.
ACCESSION   DAWBSW010000012 DAWBSW010000000
VERSION     DAWBSW010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 19886)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19886)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19886
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_600_length_19886_cov_2.661137"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1150..1159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2901..2910
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9205..9214
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12000..12009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13795..13804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19220..19229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5188 a         4738 c         4278 g         5622 t
ORIGIN      
        1 ccgtttatat taagacaaaa gtctccctgt gacaagggag actgctttaa cttttatttt
       61 ttatatgctc aatactccta ttgaaattct cagaacaaca agcgcatcgg cggaatttac
      121 ctttccgtcg ccgtttacat ctgccgttga cttagcaaca agagaaggtg ttgatattgt
      181 cttgtgacaa acagaacact ccatatgctt aagaccggtg ttgtaaaccg acggtctttc
      241 tgagtagacc caaactgccg tatgctcggt tttcggcgta gtaacattgt agctgtgacc
      301 gcaggcgcag atgtatgtat caattcccgt cgagctgcat gttgcctttt tcgtaaccgt
      361 tttggtgtaa gaatgaacat ggttgcttgt gctgctgaga ctaattgcat acattgtcca
      421 gttaatgtct ccgcactcgg gacagcccca tgaaatgtat gttgttgtat tgtgatgcgg
      481 attgttatgt gcatagtccg tcataagtcc gtcttcaccg tcgccgcaga gaattgcatg
      541 ctggaacgat ttgcaactgt tgcaatagta gaatacggga tcgccgggcg aaagcttgcc
      601 ggcattatcg tcaaaataaa tgtacggccc gtcggttttg agaacatgag ccgttccgta
      661 tgttcctttg agagcattga aaagatttcc gacagagcgc tccataacat tgattccgcc
      721 ggcttgcaag cagttggaaa caaatcctgc acaaagttca attccgttat cccagttttc
      781 ctcggcataa tcaagtgccg actgcgggtt ataatcaacg gcgcttgcat ttgtgcttgc
      841 gaacgcaaac gcagacacaa tcattgtgag ggctacaaat acggatataa ctcttttcat
      901 gttccttcct ccgagatctt tttaacgtta gtttttaaac aacggtttaa ttataacaac
      961 aaattacagt cttgtcaata agttacaaga tgttacagct gttttttctt gttctaagca
     1021 tgttttgagg tgaaaaagct atatatatta taaaaggata agcgtaatca ggctacaaat
     1081 taccctcaaa gtaacaaatc cgagtcgttt ctgtaacctt ttgtaacttt ttaggttttg
     1141 cacaaaaaan nnnnnnnnnt tttttgactg tatttgcttt gattgtctcg atattatgac
     1201 aaataatttg tagaatataa acaaaatcga tttgcttttt catttgacaa attgggcata
     1261 gattactata ataagccacg ttagttcaaa aactaatttg aatatcacgg ggttgcgccc
     1321 gaataaaacg gcttttgccg tataaaagga gcttcctaaa atggaaatga taaaaaccac
     1381 actgaagaag atcggcattg cgatctccaa agccaaggta aaggctatta tgatagctgt
     1441 tatcgtgtgc tttgccgcag caaccatcta tgctgccgct gccaattcat attcggtaaa
     1501 aatcatagcc gacgatacag agatcaatat aacaacaatg cgtgaaagcg cagaggatgt
     1561 tctcaatcag gcaaatatac tgataaacaa gggcgatgtt gttgaccttt ccgatttctc
     1621 aagcggttca aaatcaacta taactgttta cagagcttgt gtaatcaccg tttatgacgg
     1681 ttcagcggaa ggagtcaagt accctgccgt aggttcggtt gaaagatccc tcagtgaaaa
     1741 cggaatcgga cttaacgacg gtgacgagct cagctgcaat aaagaagacg ttgtttacga
     1801 aggcatggag gttgttattt cccgtgcatt ccccgtttct gttattgccg acggcgagac
     1861 ctacaagctc aatatggctt ccggcactgt tgccgacgct ctcaacaagg ctgttgttac
     1921 aatcgacgat gacgatatta tttccgcttc ccttaataca gttcttacaa agggcatgac
     1981 catcaaggtt acacgagtaa cctataaaga aagaacagtt gacgaggttc tcccgttcac
     2041 aacgcagaaa aagaataatg caaatctcta tacagaccag tcaaagaccc ttcagaaggg
     2101 tgttaacggc cgtcagaacg taacctataa ggataagtat gttgacggca agctcaagga
     2161 gtccgtaaag atcaattccg ttgttacaaa ggcggcaaca gagcgtattc ttgaggtcgg
     2221 cacaaagaga cgtccgacaa cgagtgcaaa cggcataagc actatttcga cgcttagtgc
     2281 accgtcaagc cttcagattg gcaagaacgg cgttccgacg tcgtacaaga gagttatcac
     2341 gggtaccgct tccgcatatt ccggcggcgg agttaccgca acgggcaaat ctgtaagacc
     2401 cggttatatc gcagttaacc cgaatcagat tccctacggc acaaagatgt ggatcgtttc
     2461 aaacgacggt tcgtatgttt acggctacgc ttcggctgag gacaccggcg gattcatcta
     2521 ctggtcgggc agcagctcaa cagtctgcga cctttacttt gattccgagg gtcaggcaag
     2581 cgcattcggc agacgtggag taacaattta cattctctaa tgaatttaaa ataatacgca
     2641 aagagacctc tttccgattt ggaaagaggt cttagttttg tattcggttt tattttacct
     2701 gccagaagcc aaccttcttc ataaccttgg tcagtgtctt gttggcaatg tatgaggctt
     2761 tctctgcgcc ctctttagcg cacttttcaa cgtatgcctt gtccttcatt atttcgtcga
     2821 atttgtcctg aacaggcttc acatcggcta taacgctttc ggcaacggca attttaaaat
     2881 ctccgtaacc cttaccgtca nnnnnnnnnn attcggcttc aatttcatca aagctcttac
     2941 ctgtgcagca agaataaatg cccataagat tgttgattcc gtccttgccc tcggcatagc
     3001 gcacgctcgc ctcggaatcg gtaactgccg acttgaattt tttcataata tcatcgggct
     3061 tgtcaagaac atagaccgac gcttttggat ttgtatcgga ctttgacatt ttcttggtcg
     3121 ggtcctgaag cgacataacc tttgcacccg tcttgccgat atacggcttg gggatagtga
     3181 atgtcggaga atatgtgccg ttgaaacgct ccgcaatatc acgggtgatt tcaagatgct
     3241 gcttctgatc cgcgcctacg ggaacgtaat ccgcctgata gagcaagata tcggctgcca
     3301 taagcgtcgg atatgcaaaa agtccgacat tgacattgtc ggcgtgcttt gccgacttat
     3361 ccttaaactg ggtcattctc gccgcttcgc cgaattgcgt gtagcagttg agaatccagc
     3421 cgagctggct gtgctgcggc acatgtgact gaacaaaaat tatattctta tcgggatcaa
     3481 gaccgagagc tatcatcaaa gcgtacattt caagcgactg ctttctgagc tttgcaggtt
     3541 cctgacgaac cgtaattgca tgaagatccg caacggcaaa tatgcaatcg ttgtctgcac
     3601 tcatctgctt ccaattttta agtgcgccaa ggtagttgcc gagggtcgga acagacgtcg
     3661 gctgaatcat gctcagcaca cggtcttttc tttctgtatt ttccatttaa tatcactcca
     3721 aataaaaact gggaacatta tatcataaaa aaatcgtatt gtaaatcatt attatttatg
     3781 atagaattat cgggtatgaa cggaggtctt attatgtcga attttgacgc agttcttttt
     3841 gattttgacg gcacggttgc agataccggc gagggcgttt ttctttgcgt ccgtcatgct
     3901 atcgaggttc acgggcttaa acagccgagc gattcggaaa tacgcaggtt tatcggtccg
     3961 ccgatgatag tcagctatcg cacactctat cctcagcttt gcgacgacga gattcaatcg
     4021 cttatgcaga gctatcgcga aaaatatgcc aaggtcgggc tttataaata caagctttac
     4081 gacggcatta ccgaacttct ccgaaagctc aatgaagccg gaattaaaac cgctgttgca
     4141 agctcaaagc cgcaggaagc cttaacaaat ataatcaagg tcagcggcat tgacaaatat
     4201 tttgactgca tagtcggcgc agataagaat tatacggact ccgacaaggc gacaatcatc
     4261 gaagaagcaa tcaggcgaac cgaagttacg gataggagca gggttctcat ggtcggcgac
     4321 agaaaatatg atattgtcgg tgcgcacaag gcgggagtag cctgcgccgc agtacttttc
     4381 ggctacggca gccgtgagga attcgacgaa tacaaagcgg attatattgt tgaaagcttc
     4441 aaagaggttg aaaatctcgt aatataaaca aaaacagata tataatgtga aattattgct
     4501 catttttatt tataatgagt tgtttaaaaa tgagctttgc cgaaaatttt cgagccgcac
     4561 gaaagaagct taacctcact caaatggagg gtgccaagcc ggtcggcgtt gaacgctccg
     4621 ccgtggctca ctacaaaaat gtcgtttccc ttccccacga cggcaacctt caaaaaattt
     4681 gcaaaagact tcaaataact atcgaaagaa tatttctgta attaaaaacg ctctctctga
     4741 aaaagagaaa gcgtttctgt ttttttattg attttttatt gagttacaat atccgcaagc
     4801 ttcgttccct gaacctcatc ggcaccggca accgtggtcg caccggtgtt tacattaacg
     4861 gtgttgacaa atccgattcc gttcattttg aggctgacaa tgagaaggat caaaagacaa
     4921 agtacggtga tacctatcat tgaaagtgcg gtagccttgc cctttttttc tctatcgcag
     4981 gccttaacat attgatccat gcgcttctga gcatcattaa ggtcaatacc ggttgctttt
     5041 tctgtctttt tctttgccat tgccatcaac tccgatttca agcggttaat atattataca
     5101 acaattattt tattttgtca attacggctg cacggtaatc ggatgcgact cactctcatg
     5161 ttcaaaggaa atgaaattat ttctctcggc ataagcccat ctcatatcga aaagctcaca
     5221 cgcagaggct ggcatataaa gctggtcatg aattctgagc accttggcgc aaatcgcctt
     5281 ttcttcaccg ttgacaatta ccctgtcgct gttttcgaaa aactctaccc tcttgccgta
     5341 aacctcaaga gtaattttgc ccttttcctt gagaacatat cctccgatat atccaacaag
     5401 tgtaccgaag caggcatacc atactccttc aacattttcg catttaacgc ccgactgaca
     5461 aagtcccata agggcaccct tgaaggtcat tctggtctga ttgaatttcg gcgcaattgc
     5521 atagggaact atctcatcct tgcccttgtc aattgtgcac ttgtcaacaa ttctgccgtc
     5581 ctcaacaatc tttgaagtga gggtcatttt atctccgtca acctcaacga ttgcaacaac
     5641 ggaaacaggc tcctcctcgc agtaaaaagc agaatgccaa acctttgaaa ttgctcttgt
     5701 tcccggagga ttctgattgg aattgccgag catatagtga accgtgccct gtgacggacg
     5761 ttcgaaaagt tcctcgttcc ttctcgggaa gcttctcgag aagttatgct catggcccga
     5821 gaatacgaga tcaagcatct cgaagccctc tgtcatcatg ggatatgcgt cgtaattggc
     5881 gcagtttgag cctgaatagc aaatcgggaa atgatatgta ccgatattcc actgtctgcg
     5941 gttcttcttc atatcgttta tcatccattc gttaacgtcc tcgggaccgt taatgccgat
     6001 catctggaaa tgaacgttac cgtagttgaa tgtatagttt tctgttttcc agtccttagg
     6061 accgtcatag gcatacgagc ctctgaactg attgtccaag aactcggcag gctccgcata
     6121 atatctgccc tgctcatgca cggggtctct gaagccgcgg ttatcatggt tgccgagagc
     6181 catctgaagc ggaatgctct caacaacgcc ctgcattccc tcaaatgtgc cgttccactg
     6241 aatttcatgc tggccgcagt ttgtgttgtc accaccggtg agaataaatc ttgcatcggg
     6301 attttcggca agaacctttt tgagaattcc cgtaaaatat gaataatcgg gatctataag
     6361 gtcacccttt tgctggtccg aaacgcagat aaacttaaat tttgtgagat tttcaggctc
     6421 tgttgtaaag ctgaattccc cgcttctgtg ctcatcgtcg ccgcaggtgt agaagtactt
     6481 tgtatcgggt ttgagtcccg tcagctttac ccagaacata ttgcttatgt ctatatcact
     6541 cttgaaaaca tcggtatcgg catctgtgcg cttaacctct tccgagccgt cctcacggta
     6601 aagaacataa ccgcttttaa cctcaatgct cgttctccaa gtgacgttca tcgtggtttt
     6661 tgcatcatct gcaatgctga taaaaatatg gtcgggttga ggcttagagc ctcttaacgg
     6721 ttttgttgac ataattttgc ctccttatga tatacttgaa tcgtttataa taatcgttga
     6781 ttgctgcctg ttcggcgagt tgcagaaagc cttgacggtc gccattgctt ccgctgcctc
     6841 aaagaatgaa atatcgctgt ttgaatcgag gtttgcaaga agcttttgat atatttttga
     6901 gccgttttca acattttcgt cggttatata actgtcaagc atggcacata tcagcttacc
     6961 ctgcgttctc attccgtccg tgccgagcgt tttgcaatat ctgaaatact taataagcgt
     7021 ttcacccttg attgtctgac ttccctttgt gagaatcagt gtgtaattat tgtttctgta
     7081 ctcaattctt ttgtcaaccg tgtattccaa gccgtccata tagttaattg caagggcaaa
     7141 ggtctcggca tccgaaccga catacttgtt gatctttact ccctctttct gctccactac
     7201 cgaaacaagc ttgcctgcgc cgccctcctt gagggtctgt acatagcttt tgccatcggc
     7261 ggtcttaccg tcgggattca gcggacgatc cttaataata caattgtcca tatccgcttc
     7321 gataatgtga ataaaataca tttccgaaaa gtcgcttgcc gagcaaaaaa caagaaaatt
     7381 cgtttttccg ctcagctccg tcggtgaaac aggctgactg tcggtcgtcg tttcttcggc
     7441 tttggttgtc gtctcttttg taaaaatact tttcagcgaa atctcattgt gtttgagaat
     7501 aactaccgcc gaaacagagc cgaacacaac ggcgaaagca agaaaagcca aaaagaatct
     7561 caaaaggtcg ccctttttat cctgcttgct tgttttgaat ttgtgacctc cgaatgccat
     7621 ttatatcact ttcttttcaa aattactgtt gatttttctt caatatccac catgtttgcg
     7681 gcaactttgc cgttgccgag caggtcgtgt gcatcgcacc attccgactt tacataatag
     7741 gttatcgggt gcggattgct gtttgaaata acaagtattg cctcatcgtc attctttctc
     7801 gagaaagcaa cacaccccgc gaccgcggaa agaatttcaa accttccgtc cttaaaaacg
     7861 ctgttttcat ttcttatctt gccgagcttt ttataccatt cgacaagctc gccgttttcc
     7921 ttgccccaag ggtagcagcg gcgattgaac gggtccttat atccctgcat acctgcctcg
     7981 tcaccgtaat aaatcgtagg aacacccggt aaagcgaact gcatcgccga agccgccata
     8041 agaagcttca tgccgtagtg atattccctt tcgtcaagag aatgagtgct ttgccatttt
     8101 ctgtcacggt aggcacagtt ctcgcctgcg agcgcggtaa ttgccctcat tgtgtcatgg
     8161 gtagaaagat gattcatcag cacatcgagc acttcctttg gatagttctc aactatgctc
     8221 attactcttg aagcgaaaag ctctgcgttt gcgtcacgga tgaaatcaat aactgcccct
     8281 gcgaacggat agttcatcac actgtcaagc tgcttgccga gaagatatcg gcgaagctta
     8341 ccgtagctcg atttgtttga agcgtcctcc caaacctcgc cgagaacaag tccatcggct
     8401 ttttcttcct tgaccgcctt gcggaactcg tcaagaaatt catcgggaag ctcgtcggca
     8461 acgtcaagcc gccagccgga tgcgcctagc ttcagccatt ttcgcgcaat tccgtttttg
     8521 cctgttatat attcgataaa atcagagtta tcctcgttaa cctcgggcag aatctcaatt
     8581 ccccaccagc tttcgtaatc gtccggccat ttgttaaact tgtaccatcc gtaatacggt
     8641 gattccttgg actgataagc tcccaggctg tcgtagcggg aatagcgatt aaagtacctg
     8701 ctgtcatcac ccgtatggct gaaaacgccg tcgagcatga ttcttatgcc gagctttttc
     8761 gccgattcgc aaagcgattt aaaatccttt tccgtgccga gcatggagtc gattttttca
     8821 tagttcgccg tgtcgtaacg gtggttgctt tgcgcttcaa atatcggatt gagataaatg
     8881 caggtaaccc cgagtgattt aagataaccg agcttctgct cgattccgtt cagatcgccg
     8941 ccgaaataat cgttgttgag cacctttcct tctgcggtcg ggatccagta agggtcgccg
     9001 tctctgtcgg ttcgcaaaat cctgtcagac ggaacattct gctttttcgt gcccgaggaa
     9061 gcaaaacggt cgggaaaaat ttgatagatt attcctcccc tgagccattc gggggtgtga
     9121 aagtccttat cgtaaaccgt gagctgccag tcctcaccgt cgcctttaat tgcggcaagt
     9181 ccgtttcctg cgtggtaaat cctgnnnnnn nnnngcttgt gccccatgag gtgtcatatt
     9241 caaaatgata cttgtaaagt gcagcttttt cggcggtaaa atcgaccttc caccattcct
     9301 caccgacgcc ctccatacag tcccattgca tattgatata accgtattca tcagcttcgg
     9361 ctttttttat gaccagcttt gcggcatggc agcaaaaatc acggggcaga attatacgaa
     9421 atgtaaccgt ttgatcggtt ttcactgccc cgaaaatgct tttgtgatat tctaatcttg
     9481 agttatacgg aatgtacatc aattcgtggt ttccttgctg cccgttgtaa tctcatcggg
     9541 cgtttttgtg acaatatttt caaaatattt tttgtcgatt tccgttttat cttcggaaac
     9601 agtcgtcttt tcttctacat acggtgcttc ggaaatttcg agcaaagcgt ccgttatcaa
     9661 aaaacccgca acaacaaata tgacaaagaa aacggcaata acaatatact tcaccacggg
     9721 catccaagag cttttcctgt ccgtgctgat aaaatatttc ttattgtcgc tgtaagagga
     9781 gcggaactcc gtaatgctgc ggcgacgttt attatatttt tggtattttt ccatgctttt
     9841 actcccgaga gcggcatata atataatgtc ggctcttaaa ttggatttac cataatttga
     9901 tggttgaata ccgattttaa cggtcatata ctttacttaa caagtttatg aggagatgcg
     9961 gaaatggata ttcaaccgat aagcaacaat aaatttatag tcggattgac ctgtgacgag
    10021 atgaaagacc tcgatattac atatgaagat atggattact ccaatattga aacaagacgc
    10081 gtgatttgga caattcttga caccgttcgc cgtgatactg gacgtgacgt cgatccttcc
    10141 ggcaacctga tgattgaagc ggcaccggat accgacggag gctgtctttt gatgttcacc
    10201 gttgcaagcg gaccgaataa aaagagcgaa accgtgattt caaaagcaaa tcctacacgg
    10261 atctatgaat ttgaaaatct tgataatatg cttgatttcc tgcgatttct gcccgacaat
    10321 tcacgacgga tatttacaaa aggaatcaaa taccgactgg agcttccgac ggaaacggca
    10381 aacaattttc atcgcatact aaatgaatac ggagaatttg tcggcaaaag cagtcttacc
    10441 gccgctcata cgcatgagca ttggcgggat ctttaacctc tcaatgcttc aattgttttc
    10501 gccggatcgg gcgatccgaa aatcgcactg ccggcaacaa ggacgtttgc ccctgcctca
    10561 acggctttcg gtgcagtcga ttcggaaatt ccgccgtcaa cctgaatatc aatatcaatt
    10621 ccacgctctg cacattcctt gcgcagagca acgatttttt tcatcatgtc tcccatgaat
    10681 ttctgacctc cgaagccggg ctcaacagtc atgataagaa ccatgctcag cctgtcaagg
    10741 aagggaaaaa cggactcaac cggtgtgttc ggcttaacag caaggcctgc cttacagcct
    10801 cccgaaataa ttttgtctat agtctcgtcg atcggagaat ctgattcaac gtgaaagtca
    10861 attatatctg cgcccgcctt gatgaaatcg tcaacatatt taagcggatc gcttatcata
    10921 aggtggcagt cgaaaacctt gtcgctgcac tttcttatgc acttaacaac aggcgcaccg
    10981 aaggtaatgt tcggcacaaa atggccgtcc ataacatcaa tgtggatcca gtctgcgccg
    11041 caggcatcca tgcgagcggt ttcctccccc atttttgaaa aatcgcagga gagaatcgac
    11101 ggagcaataa gtatcataag aagcaccctt tactataaat tattaactat acataattaa
    11161 acatattata taattaaatg cgcccgaggt caagaaaaat cggacgcatt tttgtaatgt
    11221 aaccactgat taaagatatt tttttatttc tgaaagtgag cacacgttaa aatcctgcaa
    11281 atcggagctt tcatttgtca gattatggtt ataccggcag gttttcattc cgaagcttgc
    11341 accgccctca atatctgcgg tcaaagaatc gcctatcatc actgtttccg acggaacaat
    11401 aggactgatt ttttcaaagc atcgctcaaa gaattccttt tggggctttg aaaagcctat
    11461 ctgctccgag ataaaaattc tgtcaagata ttcggtcatt cctgcattgt tgagccgctt
    11521 tatctgctgt gcatacgaag cgttgcttgc cgcacaaaca agatatttgc cgtgaagata
    11581 tttaagcaga tcaagcgcac cgtcaacagg ctcggcgctc tctgtcagat aagagtagaa
    11641 cacattttcg aattcaacgc cgtcaacatc aatcccgatc aatgcgaaaa tcatattcca
    11701 acggcaggca taaagctcct cttttgtcag ttcgcccttt tcaattctga gccaaagtcg
    11761 attgtttatc atattgaagg tgtcgaacat cgagttttca aagctcaaac cataatcatt
    11821 aaatgccttt tgcattgacc aaagggcaca tttgttgaaa tccaaaagag tattgtcaat
    11881 atcaataaag acagccttga tattatccat aaaatcacct tatttattgt agcatatttt
    11941 acaatcgttt tcaaataaat aaattttgca aagcaatgga cacacatgcc gaaaggagtn
    12001 nnnnnnnnnc atacgttgac gccatgaaaa agctgaatga aataggcaga gaatttaaga
    12061 aaaacaaata agcttcgata aaaaaggctg taaatccaaa agcgatttgc tttacgattt
    12121 acagcctgat ttttatattg tctcgatata atacgggcat atgtcctcat aatgtccgtc
    12181 gtccattctg aagccgccgg gaattgttcc gagctgtttg aaccctatcc gctcataaag
    12241 atgtcttgcg tgaatgttcg tcgcgacgac cgcattgaat tgcaggattc cgaagccgtg
    12301 cgctttcgcc tgagctatgc aatcccgaac cagcttttcg ccgatgtgca atcccctgct
    12361 ttcggacgaa acggcatagc ttgcatttgc aatgtggccg catctgccga cgttgttcgg
    12421 atgaagaata tacattccgt aaaccttgcc gtcctcatcg accgccacac cgcaataggt
    12481 ctgctgagca aagaattctc ttgcgctccc gtccgtgagc tcatcctcct gaggaaatgc
    12541 aacaccctcg tcaacaacct cgttccatat atctttcata gcggggatat cgtccgcact
    12601 gtattttctt acaacaatat ttttcacggt aacatcctct tcatatcaat aacattctgt
    12661 cattgtcgag gtcacgtcct gcctcttcct tgaatttctg aagcagatcg ggtactgtaa
    12721 gagcctttct ttcctcgccc gaggtgtcca tgataatccg gccgttgttc atcattattg
    12781 ttctgtttcc aagctcgaga gccgactgca tattgtgagt gatcatcata caggtgagct
    12841 tgttttcggc aacgattttt tccgtcaacg aaagaacctt ttccgcagtt cccgggtcga
    12901 gagctgccgt gtgctcatca aggagaagca gctccggcgg gttgaacgtc gccatcataa
    12961 gcgtaagagc ctgtcgctga ccgcccgaga gaagcccaac gggctggctc atcctgtttt
    13021 caagtcccat atcgagcaac gcgacacgct cacggaaagc ctttttatcc gctttcgaaa
    13081 cgtggctaag ccaaccgcct ctgcctgctg caagagcaag attttcttca accgtcattc
    13141 cgggtgcgct gcccttcatc gggtcctgaa aaagtctgcc gatttttctt gcccgcttat
    13201 actccggcat gagcgttata tcctttccgt tcaattcaat cctgccgctg tcaacaataa
    13261 aagttcctgc aatcgcattg aaaagcgttg atttgcctgc gccgttcgct ccgataactg
    13321 taataaaatc gccgtcatta accttgagcg aaatgtcgtt gagagctttt ttggcatcag
    13381 tagttccgga attaaaggtg atggaaatgt tggagatatt aagcattacg cagtcctcct
    13441 tccgaagcgc tgatcaagct tacgctttaa gaacgggaac cttgttttga gatatggaat
    13501 cgaaatcgca agcacaacga tgaccgatga aacgaattta agcatgaatg ccggcatatc
    13561 gaagcgcaga gcaatagtat aaacaagtct gaaaacaaat gcgccgagaa ccattccgac
    13621 aatctgaagc ggaattttct ttctgccgag aaaaacgtta ccgataagca atgatgcaag
    13681 tgcaatcgta accatacccg ttccgatatc aatattaacg gacttctgcg actgagcaag
    13741 cagacaaccg gaaagagccg taaacgagtt cgcaatgcaa agtcccacaa ttgtnnnnnn
    13801 nnnncacaat tgttgtgaaa acgggattga ttgaagatga gcggaccata tccgggttgt
    13861 tgcccgttgc acgaattgca agtccgagct tcgttctgag gaaaagcgca agaagaacgc
    13921 cgatgataat aaccgcaagc agtgcgacaa tcagcttata ctgctccata agaggagtgt
    13981 ttctgagcaa gtcgagcgct ttggtgaaaa cggtgtcggt tttgttgaga ttaaggatcg
    14041 atgaattgcc cataacggca atattgattg aataaagcgc cgtattgaca ataattcctg
    14101 ccagcaggct gttgacaccc attttcgtct gaaggaatgc ggtgatgaag ccagacgcaa
    14161 tacctgccgc catagcggcg ggaagtgcaa gaaacgggtg acccgagagc gtaacgaccg
    14221 caccgacggt tgccccaaga gtgaagcagc cgtcggtcga taagtcgcat acattaagca
    14281 tcgaatagct cagataaagt gcaattacgg taagtgcgct tatgagtcca agttcggatg
    14341 cggtttgaat aagtgctaaa acagaaccca gcgacataag ttaagttcct ttccgaataa
    14401 ttatttctgt ccgtcgaagc tctcggcggt cttgattttt tcaacctttg tgcagtgcgg
    14461 agcgattgcc ttcgcaacat cgtcatagtt catgccgagc tgtgagcaga tttccgtgtt
    14521 gattgttgcc gtaccgttat caaatgtgcg aacagcaacc tcagacggat tcttgccctc
    14581 aagaagaatt tcggcaatca tgtttgcggt ttccttgccg agattgatgt agtcaacacc
    14641 gaagccgagg aatgcaccgt taagagcaaa tgaatcggca cctgtgaaat gcggaatacc
    14701 tgcctttgca aagatctcat agattgaaag ctctgctgtc atgatcgagt tatctgtcgg
    14761 ggtgaaaact gcgtcaacct tatcagaaac aagagcctga gcggcgagtg taatttcatc
    14821 tgcggtcttg cctgtctttt cgacgtaagc aatgcctttc ttttcaagat atgcctttgc
    14881 gtccttgata gcggctgttg atgaatcctg acctacatcg tagaggagac cgaccttttt
    14941 gatgctcggc tttgtctcaa acatgatatc cataatagcg tttgtgttga gataatccga
    15001 agtgcctgtg atgtttgcgc ccggagcttc aagtgattta acaagctcgg cacccacggg
    15061 atcggtaact gcggagaaaa caacggggag gtttttgccc tctgttgcgt tctgcattgc
    15121 cattgcaacc ggtgttgcga cgccgaccat aagatcaaca tcatcagcga tgaagttgga
    15181 aactatctga ttaagaacat tgttgtccgc gttgcagttt tgatatttaa cgtcgaaagt
    15241 aacgcccttt tcctttccta tagcggcgag ctgtgactga atgttttcaa ctatctgatt
    15301 gagcgaagca tcgttaacat agttgcaaag tccgatggta tatgtgtttt ttgttttgga
    15361 agctgtaccg caagcggtaa gagttgcgag aatgagtatg gcacaaagtg caagagaaat
    15421 aaactttttc atgataatat caacctttct gaaaataaat gaagtgtttt taaaatatat
    15481 tcggagcaga aaatcaatcg gcgcacctgc gccaaagctc agtcaataaa atcataataa
    15541 aacctcccgt gaaaataaaa aatcccgtcc ctgcaatttc ttgccgggac aggaataaaa
    15601 atcatatcct gtggtgccac cctgcttgac gctttcgcat ccactctgac gtactaatca
    15661 tacgctgaca tttgttcacg gagagtcacc tccgtctcac atactccgaa gctaacttcg
    15721 tttccgctcg ccctcgaaag tccattcgat caaatcttct tctgccgcaa tcccaccgcc
    15781 tgcggctctc tgaaagaaca gtgttaagat ctactcactc tttctcatcg gtttagacgc
    15841 attatactac cgaatttttc ggttgtcaat agttttttta aaaaaattcg ttataatgaa
    15901 acaaattcta cgttcactac cattttattc cgccacgtct tgaaatgaca gaaaattata
    15961 tttgacaggc tgtcatatcc tcaaagctct cacgcttaac agcgacatat tcgctgtcct
    16021 tcgtcagcat tataacaggc ggctttgtga tggcattgta gttggacgac atagcataat
    16081 tgtatgcacc cgttgtcaaa accgcaagga tatcgtctct ctgcggctcg gggattttaa
    16141 ctccctcctg aatgaggtcg ccgctctcgc agcagcgtcc cgcaacagtg cattcatagt
    16201 ccgctttttc gttcattctg tttgcaacac aaacagtgta ttccgactga taaagcgcat
    16261 atctcggatt atccggcata ccgccgtcaa tcgaaacata gttcttaaag cccggaattt
    16321 ctttttttgc accgacggtg taaagagtca tgcctgcatc ggcaacaatg cttctgcccg
    16381 gttccataag gatctgcggc atattcactt tcttttcggc gcactccgct ttaaatattt
    16441 ccgaaaggct cttaatatta gccgcatagt cgatctccgg gtcgctctca acatagcgaa
    16501 cacccatacc gccgccgatg ttgagtgtct gagcctcata gccctgctcg ttcttgacct
    16561 ttgcgataaa gtcaatcatt atctttgccg catcgctgaa cggcttgtac tcaaaaatct
    16621 gcgaaccgat atggcagtga tatcctctca gctcgatatt ctcgagagaa agcgcaagcc
    16681 ttacaagctc gtccgcctga cctgtttcaa tcgccgttcc gaacttggaa tcgaccttgc
    16741 ctgttgagat tttcgcatgg gtatgagggt caataccggg agtgagacga agcagaatct
    16801 tctgcctgat tcccctcttt ttggcgtggc ggtcaattgc gcgaagctcg tcgcagttgt
    16861 cgcagacgaa atatccgata gaattatcca ttgcataggc tatatcgttg cccgtcttgt
    16921 tattgccgtg aaaataggca ttttcaagag ggaaaccggc aaccgccgcc gtataaagct
    16981 caccgggcga aacgacatca atgcccatgc cctcctcttt cataatcctg tatatctgct
    17041 tgaagcacaa tgatttgctg gcaaaaagag ggaaagagtc tttgccgaaa tactcagcca
    17101 tagccgtttt gtaaattcga catttctccc tgattttttc ttcatccatt atatatgccg
    17161 gtgtgccgta cttctcggca agctcgaccg tgtctctgcc cgcaaaagta aggtgtccct
    17221 tttcatttat tccgagattg tcgcaaatca ttttaaaaat cctttctgtt gtgcaaatta
    17281 cagcatatgt gctctgattt cttcgccgtc catatcaacg agaagcgcag gtgcgatatc
    17341 gaacaccgtt cggcagcccg attctccgtt tgccttgagc ttaaaagcag ctcttgcata
    17401 agcggcaaga acgctgcccg taaattccgg atttgattca agcttgagac tgtattctat
    17461 agtatgcttt gtgccgaggt caaagcccgt gcggccgctt ctgataacac tgccgccgtg
    17521 cggaattccg ctgtgattgg ctttcagctc atcaagtgaa ataaagttta ccgttgtatc
    17581 ataatccgag aagtagttag gcatggtttt gatctcattc tcgattctcg ccttgtctgc
    17641 gccctcctca acaacaacgt agcactctct tgtgtgcttt tctcttgttg taagctcggg
    17701 attttcgccg tttctgactc tttcgactgc actctcgaca ggtacggtat actgtcttgc
    17761 gtcaacaacg ccgtcaatcc tgcggatagc atcggaatgt ccctggctga ctcctctgcc
    17821 ccagaaggtg taatccttgc cgttcggaag aacagcattc atgtaaagtc ggttgaggga
    17881 gaaaagaccc ggatcccagc cgacggaaat aatcgcaata ttgttgcctg acttcgcctt
    17941 tgcgtcaaca ttggcaaaat gctccggaat ttttgcgtgt gtgtcaaagc tgtcgatgac
    18001 gctgaaattt tcggcaatca tcggagttgt tttcggaagg tcggttgcac tgccgccgca
    18061 gttcacgata acgtcgatct tatccttaaa gtccgctatc ttatcaaagc cgtaaacgcc
    18121 tgctccttct gtccttatct taacgctttc ggggtctcga cgagtaaaaa cggcaaccag
    18181 ctccatatcg tctgaatttt taaccgccgc ttcaactcct ctgccgaggt tgccgtaacc
    18241 ggcaatacca attctgatca tatatattat cctttctcac ggaataatta ttctgtaaca
    18301 atgctgttca tatcgtaaag tcctgccggc ttgccgagaa gaaatcttgc ggccgaaatc
    18361 ccgccctcgg caaagagcgc tcttgaatga gcctcatgct tgagggttat cgtttggctg
    18421 tcggttgaaa catagacttc atgaattccg actatatttc cgcgtcttac ggcacttata
    18481 ccgatttcat cgggttcacg cttaccgttt cgcgacctgc cgagaacaaa ttttgccttt
    18541 tggcgaacct ttttcagctc ctttgcaatc atgagagccg ttccgctcgg agcgtcaagc
    18601 tttctgttgt gatgcgtttc gacaatctca atatcggcat cgggcataac ctccgcagca
    18661 cgcttcgcaa gctcaacgag aagtgcaacg ccgagcgaca tatttgccga atggaaaacg
    18721 ggaattttct ttgccgcctc ggaaatcatt ttgagctcct catcggtgtg tcccgtggtc
    18781 gcaatgaccg tcgggatatt tttcctaacc gcatattcaa gcagctcagc tgttccgagg
    18841 tggttggaaa agtcgattat aacatcagcc tccccgtcaa aatcattgat atcgctgtaa
    18901 catccgctct tgtcggccat cttgtcaacc ttggcagcag ccttcatgtc ctccgccgca
    18961 tcaacaagct tttcgacctc acagcccatt ctgccgcatg cgccgttaat caatacctta
    19021 atcatacatt aagtccctgc tctctcatgc actgaagaag aacttccttg tgtgcgtcct
    19081 ccatcggagt aagcggaagt ctgagataat cctcgcagaa gcccattgca gccattgcag
    19141 ccttaacggg aatcgggttg acctccgaga aaagagcctt gatgagcttg aaatacttga
    19201 attgaagctc aagggcgccn nnnnnnnnnc cctttgtgtc gccttcaaag aacttcttgc
    19261 acatgagtga tgtttccttc gggaaaacat tggaaagaac ggagataaca cccgagccgc
    19321 ctaccgaaag aagaggaacg atctcatcat cgttgcccga atagaggtca agtctgtctc
    19381 cgacgattga catggtctca acgatctttg caatatttcc gttagcttcc ttgatgccct
    19441 gaatcatcgg gtgatctgca agtatgtcat aggttgcagg ctcgatattt acacctgttc
    19501 ttgacggaac gttgtaaaga atacagggct ttgagcttgc gtcggctgtt gccatgtatg
    19561 acttgatgag tccgttctgt gttgccttat tgtaataggg ggtaacaaca agaactgcgt
    19621 cgtagccgat ctcgcaagca aacttcgtca ggtcgatacc gtaagcgata tcatttgagc
    19681 ctgtgcccgc aatcatcgga actctgccgt cggctctctg aattgcgaaa tcgagagcct
    19741 ctctatgctc cttgtcgctg agagttgagc cttcgccaga ggtaccgcag attacaaggc
    19801 cgtcaatgcc cgattcaatc tgccaatcga taagtctgcc gaagctttcg taatcaattc
    19861 catccgctgt gagcggtgtg atgagc
//
LOCUS       DAWBSW010000013        19220 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_634_length_19220_cov_2.809392, whole genome shotgun sequence.
ACCESSION   DAWBSW010000013 DAWBSW010000000
VERSION     DAWBSW010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 19220)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19220)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19220
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_19220_cov_2.809392"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3596..3605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5023..5032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6398..6407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9557..9566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13126..13135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16262..16271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18980..18993
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5347 a         4469 c         4369 g         4961 t
ORIGIN      
        1 aatacttgcc ctttccatac ttgttgagct tcttctcggg atcaactgac attacatatt
       61 ttacaaaatc gagatcgccg aacggaacac gggcctcaag actgttgacc gaaatacaac
      121 ggtcggcacg aagaacatca tacatatgaa gctcacggat tctcttctgc gattccttct
      181 ggaactcctc ggctgacgga gcaaaatccg tatacttgta gccgaaaagc tcatcggaaa
      241 tctcacccgt caaaagaact cggatatccg tgttttcgtg aatccatttg caaagaagat
      301 acatacccat cgaagcacgg atagtcgtta tatcaaatgt gccgaggaga tgaataacat
      361 catcgagcga atcgagaacc tgctgtttcg tcatgatgac ctcggtatga tcgctgccga
      421 tatagtccgc aacctgcttt gcatacttga ggtcgattgc gtcctcgctc atgccgattg
      481 caaatgtacg aatcggcttg tcgctctttt tctgggcgat tgcacacaca agtgaggaat
      541 caagtccgcc ggaaagaaga aaaccaacct ttgcgtctgc aacaagtctt ttttcaacgc
      601 ctgcaacaag cttatcatgg atctttgagc aaatctcgtc aagcgaatcg tagcttacct
      661 tatcaactgc ggcaatatcg caatagcagt gaaattcgcc gcccttataa tagtgtcccg
      721 gagggaaagg catgatatcc ttgcacattt ccacaaggtt cttagcctcg cttgcgaaaa
      781 cgattgtacc cttttcgtca tggccgtaat aaagcggtct gatgccgatc gggtctcttg
      841 cggctatgta ctcgcctgcc tctgcatcat agataatcat tgcaaattcg gcatcaagca
      901 ttttgaacat atcaacgccg tattcacggt acatcgggaa aatgatctca cagtccgagc
      961 cgctcttaaa tgtatatttt ttgctaagct cttcctttgt tttttcaaat ccgtaaagct
     1021 caccgttgca gattgcatag ctgccgtcaa gctcaaacgg ctgcattcct tcgggggtca
     1081 gtcccataat agaaagacgg tgaaagccta aaagaccctt gcctgtatca acgatacggc
     1141 tgtcatcggg gcctctggat accgtcttgt caaaaccttt tttaaatctc tcaaaatcgt
     1201 cacagctgtc gcagtaaccc ataattgaac acataataaa atcctccgtg atttttttca
     1261 gcatagaata gggcaggtgc cgtaacccgc tgtgccacct atttttgttc tttggggtct
     1321 ctgtcaagac cctgtcaccg taacggttga cctccgtctc acctacttga aaatctttcg
     1381 gatcgaagct cgtcggatgt tattcatgca gcttccgctg tgcggcttcc accgttccgc
     1441 actctctgag agtgaacctc tgcattactt cttccgactc aaacgccttt agcattatcg
     1501 tctttacttt tcttatcgat caccgtcttg gtatctgact gtccctttcg ccgatgcaaa
     1561 agggacacga taccgagaaa ggataaatca ataaggagtt gtttatttta cgccgaagag
     1621 gagatcgccg taggtcggga acggccagta gctctctgcc gtgatcgtct cggcctcatc
     1681 gcaggcgata cgaagaacgg acatcttcgg aattacatcg tcacggatag cctcggaagc
     1741 ctcggtgata tccttgatcg tgttgtattt aacaagtgca atttcaagct cttttgttgc
     1801 cgcatccatt tcgtcaacaa gaacggaaag cttcttgagc atatcctcct cgtactttac
     1861 ggaaataccg gcaacggaat tcttctttgc gctgtaagct gttgcaatat cggaaacata
     1921 cttctcaacg gcaggaataa tttctttcct tgccatgtca accattgtgt tgccttcgat
     1981 gttaacagtc ttgcagtagt tttcaagagt gatctcgtaa cgtgatacga tttcggcagg
     2041 tgagaagatc ttgtgagccg taagcatatc aacatttttc ttctcaagca aggtcgggag
     2101 tgcatcggga gttgtgcgga ggttgagaag gcctctcttt tccgtagcct ctttgatcca
     2161 tgcatcgtca tagccgttgc cgttgaagat gattctcttg tgatccttga tggtcttttt
     2221 aatcatgtca tggagagcgg tctcaaaatc gtcagcgccc tcaagacggt ctgcataaat
     2281 tttaaggctc tctgcaactg cggcgttaag catgatgttt gtgcaagcga tgctgtttgc
     2341 cgagccgagc atacggaatt caaacttgtt gcccgtgaaa gcaaacggtg aagtacggtt
     2401 tctgtccgtt gtgtctctga agaacttcgg aagaacgtga acgccgagct tcataactgt
     2461 cttttctgct ccgctgtacg gcttgtcact ctcgattgcc tcaagaacct ctgtaagctc
     2521 atcgccgagg aagattgaaa caactgccgg cggtgcttcg tttgcaccga gtctgtggtc
     2581 gttacctgcc gttgcaacag aaagacggag aagatcctga taatcatcaa cagccttaat
     2641 aactgcacaa aggaaaagca ggaactgagc gttctcataa ggagtttcac ccggggtaag
     2701 aaggtttaca cctgtgtcgg ttgccattga ccagttgttg tgcttgccgc tgccgtttac
     2761 accggcgaac ggcttctcat ggaggagaca tacaagaccg tgtcttgctg caaccttctg
     2821 catgatctcc attgtgatct gattgtggtc cgtagcaatg ttggttgtcg aataaatcgg
     2881 agcaagctca tgctgagcgg gagcaacctc gttatgctct gtcttggcaa ggatgccaag
     2941 cttccaaagc tcctcgttaa ggtctgccat atattcggcg actctcggct ttataacgcc
     3001 gaaatagtga tcgtccattt cctgaccctt cggcggcttt gctccgaaaa gggttctgcc
     3061 ggtaaatttc aaatccttgc gcttctcgta catttcctta tcaacaagga aatattcctg
     3121 ctcaggaccg acggaggtgc gaacgcactt tacatcatcg ttgccgaaga gcttgaggat
     3181 tctcattgcc tgcttgttaa gagcctgcat tgaacgaaga agcggtgttt tcttgtcaag
     3241 tgcctcacca ccgtatgaac agaaagcggt gggaatgcaa agggtattac ccttgataaa
     3301 tgcgtatgat gtgggatccc atgcggtata tcctcttgcc tcaaatgttg cacgaagtcc
     3361 gcctgagggg aaagatgatg cgtcaggctc gcccttgatg agctctttgc ctgagaattc
     3421 catgattact ctgccgtcgg gagcgggaga gatgaagctg tcgtgcttct cagccgtgat
     3481 acccgtaagc ggctggaacc agtgtgtgaa atgagttgca ccgtgtgcga ccgcccagtc
     3541 cttcattgct tctgcgactg cgtttgccac gtcgggatta aggcttctgc cctcgnnnnn
     3601 nnnnnatcct tcattgcgtc ggctaccgca cttgctacct cgctgtcaag acgggcacct
     3661 tcgtctatcg tctttttcag cgagcgataa acgggaagcg gaagtcttgc tttcattacc
     3721 ctgtcgtcaa aaacaagact tccgaaatac tccggtacgg ttttcataac caacactcct
     3781 ttaacattaa attaaaaaac taaaaacggg gcagagagtc catgatactg tcatgagacg
     3841 ccgttgcccc gttaaatata ctgttgtatt gtaaatatgt ctttagcaca catcaatttc
     3901 aagccgtcca aacaccgaaa gcggcgtgct ataaactttt tcttatcgct tagagtgcgt
     3961 tagttccgag gcttggcgga gccgacatac ttaggtattt caagccgcca taacgcagga
     4021 aataacgtgc tataatcctt tgagtttatc gcttagagtg cgtcggtttc aaggcgaaac
     4081 gtcgcagaca tacacttgta tttcaagacg tttcaacgcc gaaagcggcg tgctataagc
     4141 gataagaagc aataagaaaa ggcgtcagag agcttcatta ctctcagacg ccattgcctt
     4201 tatgccgaca ttataacgca aaaaaatatt ttgtcaaccc ttttttaaaa aaatttttta
     4261 ttttttattt tcttgccgaa gcaaatcaaa atcagtcaaa tttgctttca agaatatcat
     4321 atgtgtttgc cgtttcaagt gttactcctc cgctgtattc gggcgaaacg gttttatcgt
     4381 accactgctg cgaagtttca tcggttcgct cgagcagaac ggacggagcg agccttccgc
     4441 cgataagtct gtaatcggtg attttatcct gctttcctgc gtcacggatc gcaattacta
     4501 tcccgttgcc gtcacgggat accgccatca cggaatgacc cgtaaagtca atcattgtgc
     4561 caatgtgctc cgctttgccc gtcattgaat tatagcgata aacatatata tcacgaaaag
     4621 cggatgatgc gacacgaata tcccagacga gcagctcggc tgtgccgtcg ccgtcaaaat
     4681 caaaaagcga atacatcggc agttcatccg gctcgccgtt gccgtaacgg taaatcttct
     4741 cgataagaac cgatctgtat tctgtcaaaa tgctgctttt cacatcaacg tttgccgctt
     4801 ttttagcggg tgaacatgaa cagcaagccg atatcaaagc cgcaataatt acgagtgaaa
     4861 atattttctt cattttacat ccgctcccga taaaagtata ttaaagtcaa tataaataaa
     4921 tgtctgcgaa aaatccgtga ttttttcgtc ggtttgcagc cttgttgcaa cctcaatgtc
     4981 gttgacaaaa ttcgcgtaat atgttaaaat gccgtatcaa tgnnnnnnnn nnggtcttga
     5041 cgacccctgt ccgaatgggc gtctttttct ttacagaaag gaaggtctgc catgaaacga
     5101 gagggagaaa tacgtatccc gtccggctgt gccgtttcgg cggtcatatc gaggaacggt
     5161 aacaagatga ccggcgagga tattatcaaa agtatgcttc cgatgcacga ccgttccaac
     5221 ggattgggcg gcggcttcgc aggctacgga atttatcccg aataccgcga tttttatgct
     5281 tttcatattt ttttcgacaa caatgattca agggttgaat gtgaacgctt tctcaaggag
     5341 aattttgaaa ttgtcaaggc cgagtccgtg cccatcagaa aaattcccga gataaccgat
     5401 gttccttata tttggcgcta tttcgttgcg ccgtttgcat ctgttctttc aaggcttcag
     5461 cttgacgaaa acacctatgt tgcaaggact gttatggata tcaatacccg aatcaagggc
     5521 gcatatgttt tttcaagcgg caaaaatatg ggttgcttta aggccgtagg cttccccgag
     5581 gacgtcggca gattttaccg acttgaagaa tacgagggat attcttggac ggctcacggc
     5641 agatatccta caaacacacc cggctggtgg ggcggtgctc acccgttttc gcttctcgat
     5701 ttttccgttg ttcataacgg tgaaatatca tcctacgatg caaaccgccg atatatcgaa
     5761 atgttcggat acaagtgcac gcttcagacc gataccgagg tcataactta tattgccgac
     5821 tatcttctcc gccgtcaggg tctctctctt gaagagactg cttcggttat tgccgctccg
     5881 ttttggagta caatcgaagg aatgagtgaa gaagaagccg aagaacacgc atatctcaga
     5941 aaaatgtatt caagcctttt gattacggga ccgttctcca ttatactggg ctttgagggc
     6001 ggaattatgg ctctcaacga ccgtcttaaa ttgcgttcaa tggttaccgc agaaaaagac
     6061 gataaagtat atatcgcaag tgaggaagct gcaatccgtg caatagctcc ggatgcaggc
     6121 gaaatatatt ctcctgccgg cggcgagccc ataatagtca gggtaaagga gggtgcttac
     6181 tgatgttccc gtcatatctt acctcggatt ttgaaataat aagaaatccg tctcgctgta
     6241 caaactgccg catctgcgaa aagcagtgtg caaacggcgt ccactcctat aatgccgacc
     6301 ttaaagcaat gatcagcgac gaaacaaaat gtgtcgactg tctcagatgc gtcacctact
     6361 gcccgacaag agctttgaaa atcgttaaaa acgactgnnn nnnnnnnaat gaaaactgga
     6421 caagcgatac aataaaagaa atctataagc aagccgacag cggcggagtt cttctctcgt
     6481 caatgggcaa tccgaagcct ctgcccgtct actgggacag aatacttatc aatgcttcgc
     6541 aggttaccaa tccaccgatt gacccgttgc gtgaaccgat ggaaacaaag gttttcctcg
     6601 gcaaaaagcc gtcggaaatt aaaagaaatc ccgacggtac tcttgataca aagcttccgc
     6661 ctcagcttga gctggcaatg ccagtcatgt tctcggcaat gagctacggc tcaatcagct
     6721 acaacgctca caaaagcctc gccaaggccg ccgagcttct cggaatctgc tacaatacag
     6781 gtgagggcgg actgcatgag gatttttact gctacggcga aaacaccgtt gttcaggtgg
     6841 catcgggtcg tttcggtgtt cacgagggat atctgaacgc aggtgcggct atagaaataa
     6901 aaatgggaca gggtgcaaag ccgggcatcg gcggacatct tcccggaaca aaaaccgtag
     6961 gagatattgc ctgtactcga atgattcccg aaggttcgga cgctatctcc ccggctccgc
     7021 accacgatat atattcgatt gaggatttac gccagcttgt ttattcctta aaggaagcga
     7081 caaactacca aaagcctatc atagtcaagg tcgccgcagt tcacaatatt gccgccatag
     7141 caagcggaat tgcccgaagc ggagccgaca tgattgcaat tgacggtttc cgaggaggta
     7201 ccggagccgc accgacaaga atccgagaca acgtaggaat tccgattgag cttgccctcg
     7261 ccgcagtcga ccagcggctc agagatgagg gcataagaaa cagtgtttct ctcattgcag
     7321 gcggaagtat cagaagcgcc gcagacgttg tcaaggcaat agctctcggc gcggatgcat
     7381 gctatattgc aaccgccgca cttattgcaa tgggctgtca cctttgccgt aactgtcagt
     7441 cgggtaaatg caactggggc atcgcaactc aggttccgga gcttgttgac agacttgacc
     7501 ccgagacggc gtgcggcaac ctcgtcaacc ttatgacagc atggcagcgt gaaatgaaag
     7561 agatcatggg aggtatgggc attaactcca ttgaagccct caagggtaac cgtcttatgc
     7621 tcagaggtat aggcttaacc gagaaggaac ttgaaattct cggaatatca cacgccggag
     7681 agtaaaaagc tttccgaaaa gcaaaaaatt ttttacaaat actttaaatt gaaataaaag
     7741 gtgtgatata atatgttgat agatgcaaaa gggcttgatt atgcagccct caataagctt
     7801 ataaaaagct cggacgacga ttgcaaaatc gagaactgcc tgggtcaaag attcatcgcc
     7861 gacggactcg caaaaggcag tgtcacgatt gacggaatac cgggcaatgc actcggcgct
     7921 tacctcaacg gtgcgcagat taccgtcaac ggcaatgcgc aggatgcggt cggagacaca
     7981 atgaatgacg gaaaaatagt cattcacggc agcatcggag acacggcagg atatgcaatg
     8041 cgaggcggcg aaatttatgt caagggcaac gcaggttacc gcgccggaat tcatatgaag
     8101 gagtaccggg acaaaaagcc gataattgtt atcggcggca gaacgggaag cttcctcggc
     8161 gaatatcagg caggcggtct gataattgtt ctcggcagaa ataccgacgg caagccgaca
     8221 gtcggcaatt tcccgtgcac gggaatgcac ggaggcaaaa tgattctcag aggctcggtc
     8281 aaagggatta aattcccgaa tcaggtaacg ctcagagatg caacagccga tgatatggca
     8341 gagatcatgc cgttcataaa aaactattgc gaatacttta actttgatgt agacgagctg
     8401 acgagcgaaa aattcaccgt tataaccccc gacagcaaaa aaccatataa acagatgtac
     8461 gttgcaaact gaaaaaggta ggtaactgtt atgaaatatt cacccgatga aataatgcaa
     8521 tatattgagg aagaggatgt taagtttatt cgtcttgcat tcacggatat ctatggcaag
     8581 cagaagaaca tttccgttat gcccgacgag cttgaaaaag cgtttcgtta cggaatagca
     8641 attgacgctt cggcaatatc gggcttcggc tgtgacgttc attccgatct gcttcttcat
     8701 cctgatccgt caaccattgc cgttctccca tggagacccg atcacggcag ggttgtcaga
     8761 atgttctgct caattacccg tcttgacgga acacctttcg gctcggacac gagagcaatt
     8821 ttaaaaaagg ctattgcaga cgcaaaaaac gagggaataa gctttgcttt cggtcccgaa
     8881 atggaattct atcttttcac ctgcgatgag gacggtaatc cgacgaaaaa tccttatgac
     8941 aacgccggat atatggacat tgctcccgac gacaagggtg aaaacgtgcg ccgtgaggtt
     9001 tgccttacac tcgaaagaat gggcataaga cccgaaagct cacaccatga ggagggcccc
     9061 ggtcagaacg agatagattt cagatattcc gagccgctca cagccgctga caatgcggta
     9121 acattcaagg cggtagtcaa cacaatagca gcaaggaacg gactcaaggc cgatttttcg
     9181 ccgaagcctc ttgaatcaat gcccggcaac ggtatgcaca tcaatctttc ggcaaaatcc
     9241 gaggacggag aggacatcat gccgcacgtt atcgcaggaa ttctcggaca tatctgcgag
     9301 ataaccgctt ttcttaacac aacatcggat tcgtttaagc gtttcggaaa caacaaagct
     9361 ccaagatata tttcatggtc aagtgagaac cgttcacagc ttgtccgtat tcctgccgca
     9421 tacggagaat acaagagagc cgagcttcgt tcacccgacc cgatgtgtaa tccgtatctc
     9481 gcctttgccc tgctgattta tgcgggtctt gacggcataa aaaataaaac agagcttccc
     9541 gaaatggcgg acataannnn nnnnnnacat aaatcttttt aatgcaccgg aggatgtcac
     9601 caaaaaattc aaaaccctgc cgagcgacat tttcagcgcc ggcaaggcag caagagaaag
     9661 cgaatttgtg aaggcgcatc tgcccgagtc gatcatagat aattattgta agtaaagcgc
     9721 aattcgcaca ccaaaatgtg cggtattgcc ttaaaacaga caaaaggagg ggagccgaat
     9781 ggattttgca caacattcct acaaagttct gatagtttca tcgtcggaca aattcgttcg
     9841 accgatgctt gatatgctcc ccgaaaaaat gtttgagccg attgaaacgg cgtccgacgt
     9901 tgcgcaggca aggcggaagt tacttgaacg cgagtttgat attgtaatta tcaactcgcc
     9961 gcttcgtgac gaattcggaa cgaaacttgc gcttgatatc tgccaaaaga gcagcgcagg
    10021 tgtcttactt ttcgtcaagg cggagcatta ttccgatatc acgtcaaagg taatgcaata
    10081 cggtgttctg acaatatcca agcccacctc gccgtctatg ataatgcaat ccgttcagat
    10141 gctttgctca acgagagagc gactgaggat aatggaaaag aaaaacgcct cggtcgaaga
    10201 aaaaatcgaa gaaatccgaa tagtaaacag ggcaaaatgc cttttgatag aacagcttaa
    10261 aatgacagag ccggaggcgc acagatacat tgaaaagcaa gcgatggaca gatgcgttac
    10321 acggcgaaaa atagcggaaa atataatttt gacttataaa taaacggagg gacatttatg
    10381 acaaaataca tctttgtaac aggcggcgtt gtttccggcc tcggcaaggg aatcacggcg
    10441 gcttcactcg gacgacttct caaggcaaga ggactcaagg ttgccgcgca gaagctcgac
    10501 ccctacatca acgttgatcc gggtacaatg agcccgtacc agcacggtga ggtttacgtc
    10561 acggaggacg gcgcagagac cgaccttgac ctcggtcatt acgagagatt tattgatgaa
    10621 aaccttaaca agtattcaaa cctcacaaca ggtaaggtct actggaacgt gctcaacaag
    10681 gagcgacgcg gcgaatacct cggctcaacg gttcaggtaa ttccccatat caccaacgag
    10741 atcaaggact ttgtttaccg tgtaggtaaa aagactgacg cagacgttgt tataaccgaa
    10801 atcggcggca caatcggcga tatcgagagc cagccgtttc ttgaggcggt aagacagatc
    10861 tctcttgaag tcggcaagga aaacagtctt ttcattcatg ttacacttgt tccgttcctc
    10921 cgcggttcgg acgaacataa atcaaagcca actcagcact ccgtcaagga gcttcaggga
    10981 atgggaatcc gtcccgacat cattgttctt cgctgcgatg agccgcttga agaatcgatt
    11041 ttcaagaaga tttccatgtt ctgtaacgtt aaacccgatt gcgtaatcga gaatatcaca
    11101 cttccttatc tttatgaagc accgctcatg cttgaaaagg caaacttctc ctccgttgtt
    11161 tgccgcgagc tcaatatcga cgctcccgcc ccgaatctct cagagtggac aagccttgtc
    11221 gaaaggatca agaatcgcag aaacaccgta catatcggac ttgtcggcaa atacgttcag
    11281 ttgcatgatg cttatctctc cgttgccgaa gcaatgcgcc atgcaggcta tacgctcgac
    11341 acgcatattg acataaactg gatagattcc gagcagctca ccaaggaaac ctatgaggat
    11401 atgctcaaag gccttgacgg aatcattgtt cccggcggat tcggcggcag aggaatcgaa
    11461 ggtatgatcc ttgccgcaaa gtacgcaaga gagaacaacg tgccgtattt cggaatttgt
    11521 ctcggtatgc aaattgccgt tatcgaatac gcaagaaacg tctgcggaat ttcagacgca
    11581 cattcgggcg aatttgatga aatatgcaaa aataaggtta ttgacttcat gcccggtcaa
    11641 agcgaaaaca ttgacaaggg cggcacactc cgtctcggcg catatccctg cgtaattgcc
    11701 gagggcacaa ccatggaacg ttgctacggc acgaatgaaa tttccgagag acaccgtcac
    11761 cgttatgagt tcaacaacga ttatcgcgat atactcaccg aaaacggtct tgtcctttcc
    11821 ggcaagtccc ccgacggcag acttgttgag actgtcgagc tttccgaccg tccgtttttc
    11881 gtcggtgttc agtaccaccc cgaattcaag tccagaccga ataaggctca cccgctcttc
    11941 aagggcttca ttgaggcagc gttgaagatg cagagcgaga aataatccgg cggtttatcc
    12001 acccaacatt gactttttgc aaggcaaagc aaatctgttc gccttataaa cagctatcaa
    12061 gataaagcag caccccgaag cacatgattg tatttcgggg tgcatttttt attatcgtct
    12121 caccacatgg agcacgccag ccgggtttat aactcattag tgtacgccgt atctgttttc
    12181 cgaaaattcg caatttcata aagctttcac tcggtttttc cgagactgat aaacgttcga
    12241 tattccgtcg actctgcaat gaaattcctt tcatctgccg tattcggcgg atattcttat
    12301 ttttagaata ataaataata cctgaagccg aaaaagtcac cctgcctgcg gcagaatgac
    12361 ttttatctgt ttgctgattc taagatcaaa tagccttatc ttccgaagat gaaatcaaaa
    12421 atacccttga taaactcaaa aacatttctg aagaagtttg ttgttctcga aataacttca
    12481 acttcaacct cagccgttgt gcctgaaatc ggctcggtta ccgtaagcgt tgtttttccg
    12541 taatgcttgc cgtaaacggt gattgaaccc gtctccggat tatattcggc agtcgcaatt
    12601 ctttcattgc cgacggaaac atcaagtctg tcgtagttga acatagaacc tatcggataa
    12661 acctcgggaa aaacgttctc gcttgtacgt ttaataacag tgatatcgtc aagttcaatt
    12721 acatcgggag aaatcgtttt gccgtcaacc acatgaaata cggaatcaac cggcttatcg
    12781 ttctcatcat agctgccttc tacatatatg ccgtaaagct ctccggcctt atatttaaaa
    12841 ggtacatctg ccgggaatcc gtcggtgtca atagagccgt agtaaatatt tccggacgta
    12901 tccataaccg aaatattcgg gcactcataa gaccagatat cgcaaactcc gtcctcagtc
    12961 ggcttaaaaa taaagtattc ggtaccgttt gccttatagg attcgcctat tttgatctcc
    13021 ttgagatcct gtactctcgt gattttgaag gtaacggttg tttcggtatg tgtgatattc
    13081 tcttcagaat ccggcaaacc tatttcaacg gaaacatcta aatcgnnnnn nnnnntcaaa
    13141 atttgaaaaa tccaaaatgt cttcctcttc cgaaacatcc ataaaataaa gatgtattga
    13201 agaaaagatt ttttcctcgt ttccgacaac aacagatgcg atgccgaaat ctttttcttt
    13261 gaacttaatt gatgcgctga ggcgatatgc tccgtcctcg ggtgcggtaa acgtgtagtt
    13321 cgccgtatcg tttttgagag taaccgtgta ctctttgtca agctcgattg atgagccttt
    13381 ttctgcaaat gcgattattg aaaccgaaaa catcataaca accgcaagta caacactgag
    13441 tatttttttc atagaaattc tcctcctgtt ttatttgggg caatgtcctc accctcaaat
    13501 attttataac ttattttgcc ataaaaatca atagctttta catttttttg acagaattac
    13561 ataattttat attcagaagc aatattttaa aaagcttttg caaaaaacaa tccgcatccg
    13621 tataaacgtt ttattaaatc tttagccgtt gtgcccacat cgcaccgagg agctacgatt
    13681 taatttgctt ttaggcagaa attttcttgc ctccccttga tttaatcaag aagaggcaag
    13741 ctattatata tttacatcgt aaattattat aaattaaacg gtagaaatgc tattgcaacg
    13801 gcaaagacaa gcgacggaag caggtttgca accttgattc tcttatccca tacaagatta
    13861 actccaacgc agaaaatcaa aatcgagccg atgagagaaa gatttgccaa tgcgccgtcc
    13921 gtcatcagcg gcttaataag ccttgcaagt gcggtaatca atccttgaaa aacagcaaca
    13981 ggtattgccg aaaacgcaca gccctttcca agggagcagg tcataaccgt aataataatc
    14041 aagtcaagta tcgctttggt aacaagtatt gaataatcgc cggaaatccc gtccttaatt
    14101 gaaccgacca ccgccattgc gcctatgcaa accgtcagtg aagtggtcac aaagccttca
    14161 acaaagcttt tgtcctttgc atttcctgtc ttaactttca gccattcgcc gaaacgctca
    14221 aagccgtgct caatattaag aatctcaccg accaaagctc cgagagcaag acttgcaaca
    14281 ataagcattg aacgtcctgc cgaaagcgac gagcctgaga gcttgagcat accctccatc
    14341 gctcctgcaa ttccgataaa aagtacgcat ataccgctcg ctttttgcag cgaatcctga
    14401 attctttcgt taagaatttt gccgaacaaa tggccgaaca ctccgccgac aattattgcc
    14461 gcagaattta tgattgtgcc gagacctacc aaaacaaact acctctttaa aattacagct
    14521 tttttattcc cgaaattctt tgaacttcca ccatattatc atcaatattc attatctttt
    14581 tcgagtcatt aaatctgccc tttgcattgt tgggattatg tattatggtg tcaaaaaacc
    14641 tgacaatcca aaacaaaggc aaaaaaaatt ttttattctt caaaaccgga taacgaattt
    14701 tcatctcatc ataattaaga aaaattattc cgaaaatatt ttttattttt tttgaatgaa
    14761 tatcttcatc aatattatcc aaaataaaac tgttttgcat tgatattccg tcaatgccgt
    14821 aaactccgcc ggacactata tattccgaca tgatatcaaa ctctccgtca aagccgcccg
    14881 aataccaatc atatgtaatc tttctgattt tgttgtaaaa aacaagcagg ccgatttttt
    14941 caagctcgcc gtttatatag tcaaaatcga gcgacggcag agacttttca taaatataca
    15001 aatcgagcag ggatcgaatt cccgttccgc cgtagcgata atgctttgca atatgagcga
    15061 caagaaaaac ataaaaatcc tcgtctgtga gcttgtattc gtattcactg ccttcgctct
    15121 tgtgcggcaa gcggaagccg tccccgtagt aagccgacag cgtctcatgg cagctatcca
    15181 ccattgagcg atgaaattca atatatacgt tgcctctttt tacattagag tgcaagttgc
    15241 cttcgccggc aaattcaaat ccgtcggcca tgaaggcttt aacaacatca tcacagcggg
    15301 ctgcatcaac aagaatatca atatcaccca ttgtccgcat atcgggagag ggataaaaat
    15361 ttttcatcac cgagcctttg aggagcatat gacgaacacc cattttatca agcttttggc
    15421 aagcgtcaca agcggcaagc tcctgttgag cctccacgac catcttttgc atacgaaatt
    15481 catcgagaaa tctcattgtg agttcatccg gtttgatttt aagcgactct gcggcatagg
    15541 caacaagatt aagtacactc tgcttctttg cgaagtcaat tatcccctgc cagtcgatga
    15601 tctcgttcac cggcagagca aagccgttaa ccgtcgacgc aatcaatgcg ataacatcgg
    15661 cggcagttct tttttcttca accgtaagca tcaaaaatcc ctcgtttata taaattacgg
    15721 ataaattata tattatagaa agaaaaaagt ctatactaat tttgattttt atattgacat
    15781 taacggcaat ttatgataga atatccgagc attaggagag ataccgaagt ggttataacg
    15841 gaacggtctt gaaaaccgtc gtatggcaac ataccgtggg ttcgaatccc actctctccg
    15901 ccactttgca ggacttccga tttcggaggt cctgcaacaa caaaatagta actgttttgt
    15961 taccaatgga gaagtactca agttggtgac gaggcgcccc tgctaagggc gtaggtcggg
    16021 taaccggcgc gggagttcga gtctcccctt ctccgccatg aaaaaccact tgcatcttgc
    16081 aagtggtttt ttcaaattaa gcgcacctta cggtgcatga aagatgaagc agggcttcgc
    16141 cctataatgc gtggtatctc cacgccgaaa aatactggcg cttcattaaa aacacagatt
    16201 acagttcgta tcgggagaga taacaatgag ttatataagt gaaatgagaa aatatatcgg
    16261 cnnnnnnnnn naaatcggtt ccgaaattgt tattttttcg ggatatttta gctctttcgt
    16321 caaaaatgtt tattttgaat tacaatcttt caagttcggt tgttttatgc gtaataatat
    16381 gctataatac aaaatatcat gattgtatat gggggtttat caatgaatta cgatgttgcc
    16441 gttgtgggcg caggtgtcat cggttcgctc atcgcaagag agctgagccg ctatgatatc
    16501 agcgttgccc ttgttgaaaa atgcaacgat gtggcaatgg gaacttcaaa agcaaacagt
    16561 gcgattgttc acgcgggttt tgatgcaatg cccggcacgc ttaaagcgcg cttcaatgtc
    16621 aggggaaccg agcttatgcc ggagatttgc aaaacgctga acgttccttt caaaaatata
    16681 ggctcgcttg ttgttgcctt ctcagacgaa gaaacagaaa ccttaaacgt tcttcttaaa
    16741 agaggaaagg cgaacggcgt tccaaacctt gaaatctatg atgcggacaa gctccgcgag
    16801 gctgagccga atatcagcga cgaggctaaa gcggctcttt gggcgccgac ggcgggtatt
    16861 gtctgcccct atgagctgac ggttgcggca acggaaaatg ccgttataaa cggtactgat
    16921 tttattcgta actttaaggt tgagaagatt gattttgacg gcaacggatt cacccttgtt
    16981 gacggtgaaa aatcaatcgg cgcaaaatat ataatcaacg ccgcaggtgt ttacagcgac
    17041 gagatcgcag cgcttatagg cgacgattca ataaaaatta tcccgagaaa gggcgaatat
    17101 atgctctgcg acaagtcggt cggttcactc gcaaagcata caatattcca gtgcccgtcc
    17161 aagatgggca aaggagttct tgtcaccccg acggttgacg gcaatctgct catcggtccg
    17221 agtgcacttg atattgagga taaggaggac gtttcaacaa cggcgagtga gcttgccgat
    17281 gttctcaatg ctgctcaaaa atctgtaccg acggtttcca caaggacggt catcacctcc
    17341 ttcgcaggtc tcagggcaca cggtgtcaat gatgatttta tcattgagcc gagcaaaaag
    17401 aacgaccgtt tctttaacgt tgcaggtatt gaatctccgg gcctttctgc cgctcccgct
    17461 attgctgaat atgtcggaca aacagtagcg aaggcaatcg gcgcggagga aaaaaatagc
    17521 tttattcccg agcgcaaggc tcccgtgcgc ttcagaaaca tgagcaatga agagcgcaag
    17581 gagcttgtta agaaaaaccc cgcttacggc agaattattt gccgctgcga gacagtcact
    17641 gagggcgaaa ttctcgacgc aattcatgct ccggctggtg cacgagacgt tgacggagtt
    17701 aagcgcagaa cgagagcggg catgggtcgc tgtcagggcg gcttctgtgg ttcaaaggta
    17761 gtcgaactgc tcgcccgtga gcttggcgta gaaatcaatg aaattacaaa attcggcggc
    17821 gagtcgaaaa ttctctatga cagaacaaag tgaggcggga attatgctta attatgatat
    17881 agttgtaatc ggcggcggtc ctgccggaat ggcagccgca atcaaagcaa aggaatgcgg
    17941 cgtcgacagc attcttgtac ttgagagagc cgagactctc ggcggaattc ttgagcagtg
    18001 catacatacg ggcttcggac ttcattattt cggcgaggag ctttcaggcc ccgaatatgc
    18061 cgacagattc attcagcttg tgcacgaaaa aggaattgaa tacaaaaccg atacaatggc
    18121 acttgaaata acggacggta acattgttta tgcggtcaac aaggaggacg gtctgcttga
    18181 aattcaggcg aaggcaatta tccttgcaat gggctgccgt gagcgtccga gaggtgcgct
    18241 gaacattgcc ggaacaagag cttcgggcgt tatgacagca ggcacggcgc agaaatatgt
    18301 caacatagac ggttacatgc cgggcaaaaa agttgttatc ctcggttcgg gagatatcgg
    18361 tcttatcatg gcaaggcgta tgactcttga gggtgccgag gttaaggtcg tgtgcgagct
    18421 tatgccttat tcaagcggac tgacaagaaa cattgttcag tgccttgacg atttcaacat
    18481 tccgcttcgt ctgagctgta ccgttatcga ggttcacggc aaagacagag tcgagggcgt
    18541 tacgattgcc gacgttgacg aaaaccgccg tcctatcaag ggtacggagc agtacattga
    18601 gtgcgacacg ctcatgcttt cggtcggtct tattccggaa aacgagctta caagagctgt
    18661 cggcattgag cttgatcccg ttacaaacgg tgcagtcgta aatgagctca gagagacctc
    18721 tcacaagggt gtttttgcct gcggcaacgt tctccacgtt cacgaccttg ttgactacgt
    18781 tactcttgaa agccagactg ccggcgaggg tgcggcaaga tatgtcctcg gtcagacaag
    18841 cagccatgaa tatgtaaaaa ccaagggagt caacggagtt cgctacattg ttccgcagaa
    18901 agtggatatt aaaaacgaga atgacgttaa gctcttcttc cgagtcggtc agacctataa
    18961 aaatgcaaaa atcgtcgttn nnnnnnnnnn nnnatataac ggcgaggaaa tactcagccg
    19021 caagaagccg agacttgctc ccggcgagat ggaaaacgtt gttattaaaa atgacgttct
    19081 caaaagcttt gaacacgacg gtgtaattga aatttcggtg gagggttgat ttatgcagaa
    19141 gaatattatt tgcgtttcat gccctatggg ctgtggaatt acagtcgagc tgaatgacag
    19201 cggcgaggtc gtttccgtaa
//
LOCUS       DAWBSW010000014        19066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_639_length_19066_cov_2.546526, whole genome shotgun sequence.
ACCESSION   DAWBSW010000014 DAWBSW010000000
VERSION     DAWBSW010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 19066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19066
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_639_length_19066_cov_2.546526"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6851..6860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7535..7544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8911..8920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12315..12324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15333..15342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15938..15947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5568 a         3916 c         4477 g         5045 t
ORIGIN      
        1 tattttcagg gcctatgcgg aatccggcga tttccacatc aaggagcttg ttgcatacgt
       61 tattgaacga cttatgaacg gcaattttac gattgaaagc tcaatgcccg acaggggtat
      121 cgttacccta cttcaacaga acgacagaaa gatcgttcac cttctttatt ctcacacgac
      181 ggtgcgcggc aagaatacgg aggtgatcga ggatatagtt cctctttata atgttgacgt
      241 cagcgtgaaa tgcaacaaac cgaaaagagt tattctcgtg ccgcagaatg aagaaatcag
      301 ctttaactac tccgacggaa gagtctcatt tacggtaccc aaggttgaaa tacatcagat
      361 gatttcgatt gagtagaaac atacattata ataataaaca aaaaggagcg gaaagccgaa
      421 aagctaaccg ctccgattta tttataagaa acccataaaa atatattctt gaagcaataa
      481 atcccaaaac cgcaaaaatc accgtttttt actgccttaa atcaggtata gaaaaagccg
      541 caagcccttg atacacaaag gcttgcggct tggagctact ggtcggactc gaaccgacga
      601 cctgctcatt acgaatgagc tgctctacca actgagccac agtagcatct gctgaaagtg
      661 tcaacgactg atattctaat atattttgga gaaaaaagca atagttttta taaaaaaagt
      721 tattattttt aacgcaacgg gcttttttta caatgcactg ctattgtttg ctcaaagttg
      781 acaggcactt ttattttgag tataatgaaa acagagaata attatgagaa gaggtgcaca
      841 aaaatgtctg aaattattgt tgccggagca ggtcacggcg gacttgtagc agcatggaag
      901 cttgcgcagc tcggtcacaa agttaccgtt tatgaacgct atgcgcgaga aaacctcggc
      961 tatgaccaaa aggatccggt ggattctgcg tattttgatt atgcagggat cacatatccc
     1021 gaggagtggc acgctccgaa taatgttata acgcttgttc cgattgatga aacagttgcc
     1081 cccgttacac ttccggagga gaaatctgct tcgactctca tcgttgaacg ccgtgagctt
     1141 ttagcttttc tcattgatct ttgcgaaaag gcaggggtgc actttgcgtt cggctgtgag
     1201 gtgcgtgaac ctgttgttct cggcagccgt attatcggca taatgacatc gcagggcata
     1261 agacttgccg atcttgtaat tgatgcctgt ggaattgatt cgccggtgcg tcggaatttg
     1321 ccagatttta ctcatgtcca aaaggagctt ggacgctttg accggcttca tgtttaccgt
     1381 gcatattata acaggattga gggcaagccc gatcccgaac acagatatta tgtctacgtc
     1441 aatgcaaacg gaacggtcgg cttcacatgg cttataaccg agcctgacag cgcggacatt
     1501 cttattgcaa gattcggaga agtgaccgaa gcggatataa aagaaaatct tgattttgtt
     1561 tgcgaaaaaa acgagcatgt cggacgtgag cttgttcgcg gaggtaaggt ggtcgatatc
     1621 cccgttcgtc agccgttggc ggtttttatt gccgacggtt atgccgcagt cggcgacagc
     1681 gcatgtatga caatttcgct caagggctcg ggaataggat attcaattaa ggcaggcacg
     1741 atacttgcga gagcggtcga ggcggacagg gacggcttgt ttaactgcga tacgctctgg
     1801 aaatatgaac gtgaattttt ccatgaaatc ggatttgccg catgtacgat tgcgctggaa
     1861 aaaaatcttc ttccatatct cacggcgaag gatgtcagcg attttatcgc aagcggaatt
     1921 ctgaccgccg aagagcttgc cgagcttaac acggacaaaa ttgaatatct cagaaaaaat
     1981 aaaattgttt caactgtcaa gaataagctg aagcttctca atgaaatgcc ggagttgaga
     2041 aacaagggac ttaaaattgt cggatggttt ggcagggtga agcttgttga accgctgttc
     2101 ccgaccaaat atgaccgaga tgacgttgaa aagtgggcgg agcactacaa tgagttcttt
     2161 gaatcaatca agacagatag aatacccggt gaggataacg aataaaacat caagggctgt
     2221 tgcttcggcg acagcctttt ttaaaataac tcttgaaaaa gcgaaagaaa gaatataaaa
     2281 tagtttatat gattttttgg ggttgataaa ttgaaggata tgaaaagata tttgaaaatg
     2341 gcggcaccgt acaagggcgc attgatattg accgtcctcg ccttgctcgg atcctccgcc
     2401 gtcagtcttg ttatgccgga tgcggttcgc aggctgacgg gagccttgac tgacggaacg
     2461 gcgaccaagg aacgagtcct tgtgtttgcg gcactaatac tttcatcgta catagtcaaa
     2521 gcgctgctga cattcgtatc aaaatacaag gctcacgttg ccgcatggaa tttcgtcggc
     2581 gatatgatgc ttgatgttta taataagctg caatcacttt caatgagatt tttcggcgat
     2641 aaacaaaccg gcgaaataat gagccgagtc ataaacgatt caaggaacat ggaaacgctt
     2701 atcgcacatg ctttgcccga tcttctttca aacattctca ttgtcatatt ggttgcaata
     2761 atgatttttt caatcaatcc gattcttgcg gcaatttcgc ttattcccag tccgctgatt
     2821 ttattcgtaa caatgaaatt ttccggcaag gttcacgctc ttttcaaacg caaccaagag
     2881 gtgcttgccg aggtcaacgg caaggttcag gacaatattt cgggaatccg tgagattcaa
     2941 tccttcggaa gagagaaaga agaatatatg agcatgaagg agtattgcaa gcggtacagt
     3001 tttgtcaatg tccgtgcaaa ttttgttgct tcgattttct tcccgacaat tgaatttctt
     3061 acttcggtcg gttcggtact tgttatggga atcggcggaa cgcttgcgct gaaggatgtg
     3121 ctttccgttt ccgacgttgt aggtttcttt atgtatcttt ctcttttcta ttcgccgata
     3181 gcggcgcttt caagaattat tgaagatatg cagaatgcat ttgcaggcgg tcacagagtg
     3241 ctgagtattc tcgatatgga gtcggaaatc aaggacagcg agaatgcaga tgacatcggc
     3301 agggcaaatg gcgagatgga gtttgatcac gtttcgtttt attataacga gaatgagcct
     3361 gtgcttaagg atatttcatt taagattgat gcaggaaaaa tggttgcgtt tgtcggagct
     3421 acaggcgtcg gaaaatcgac gatcgtttcg ctcatggaaa gattttatga ccctgtcagc
     3481 ggtacggtgc gtcttgacgg caaggatatc aaggatataa cggtcaaatc gctcagagaa
     3541 aacatttcaa tggtcttgca ggatgttttt cttttcaacg gttcaattta tgacaacatt
     3601 gcttacggaa atccctcggc gaaccgcgag gatgtaatca aagcggcgga aacggcgaga
     3661 gtgagcgatt ttgtgtgcaa aatgcccgat ggatatgata caataatcgg cgagagaggc
     3721 gttcgcctct cgggagggca gaagcagaga attgcaatcg caagagcggt gcttaaaaat
     3781 tcaccgattt tgattctcga cgaggcaacg tcggcggtcg acaacgagac ggaagcactc
     3841 attcagcagg cgattgacga gctttcaaag agccgcacgg tgatagtcat tgctcacagg
     3901 ctttcgactg ttatgaaggc ggataatata attgtgctcg aaaacggcag aattgccgaa
     3961 cagggcacgc ataatgagct tctgaagctc ggcggaattt acgcaaagct ttgcaatgtt
     4021 aattcggaca gaattaaggc tgagacgtga taatataata tgaaaggaaa attaccatga
     4081 tagtaacaac gattgacaag ctggattctt acagcgaaat tccacatgca aaggacatta
     4141 tggactttat tgaaaacttc aaaaagacgg acatgaagcc cgacagatac gatattcacg
     4201 gcgacgacct ttttgccgca gtcagccgct atgataccga gccgagggag cagagaaaat
     4261 ttgagagcca caggaaatac atagacctcc agatagttct cgacggcagt gaggaaatgg
     4321 actgggcacc gattgattcg ctcaatatga cggataacgg ctttgaaaga ggcgacgata
     4381 tcgctttcta tgagggcacc gagctttcga cagtcacact cggcggcgat cagtgcgcta
     4441 tacttttccc tgaggacgga cacagaccta atattatgca caaaaacatc aacaatgttt
     4501 tgaaaatagt atttaaaatc agaaaataat aatatttatg aataaatatt cgttttttgc
     4561 ttgacatatt caatataaag tggtatattg ttcacgtcaa aaaaacagag gaggacaaaa
     4621 ctatgaaaaa atttatagct attgtaatgt cattagtact tattgcaagt gttgcaatgc
     4681 ttgcaggctg cggtaagaaa gacgatgtta aggcagcaaa ggttattggt atcgacctta
     4741 ctaatgaaaa ctatgcattc ggtgttgata aggcacagcc ggagcttctt gaaaaaacaa
     4801 acgcatttat tgacaagata ttgagcaacg gcacatttga tgagatttgc tctcgttact
     4861 ctagcggtgg tgagccggtg cttgttaagt cggccgctaa ggattcttca aaggatcagc
     4921 tcgtcgttgc tacaaatgcc gcttttgagc cgtttgagta cactgtaggc gattcatacg
     4981 caggtattga tatggagatc gctaagcttc ttgccgatga gcttggtaaa gagcttgtta
     5041 ttgataacat ggactttgac gctgtttgcc tttctgtagg tcagcacaag tgcgatatcg
     5101 caatggcagg acttacggtt aatccggaca gagaagcatc cgttacattc tcaaagacat
     5161 attacaaggc atctcagaga gttgttgtta agggcgacga tacaacattt gatgactgca
     5221 agacagccga tgatgttctc aaaaagctcc agagctttga cgcttctgtt aagatcggtt
     5281 atcagaacgg tacaaccggc ggtatgtaca tcaacaatga gagcgactac gcctcaaaca
     5341 acctcaaggt tacgggcaag ggctacaaga acggttctct tgcaattcag gaccttatca
     5401 acggaaacat caactacgtt attattgact ctgctccggc agacagcatt gttaagtcaa
     5461 ttaacgaagt tgcataatta aagtggaata aaagcaaagc cccactgaat aagtggggta
     5521 tttgttctta taagaggatt ttttatgggt aattttgata tcaaaatcga aaagtttctc
     5581 aaagccttta tcgacaacgg cggttatcac aacgttctca cgggcttgaa gaatacaatg
     5641 gaaattgcaa taatcggttt gctcatcggt attgcaatcg gtacgctgat tgcatcgatc
     5701 cgtgttcttc cgaaatacag gacgcttccg agagttctcg ataagatatg ctcgttttat
     5761 gttgcgattt tcagaggaac gcctatggtc gttcagcttc ttgttttcta ttatgttctt
     5821 atgccattga tgggaattaa gattgacggc ttgaatgttg cgattacggt tttcggactg
     5881 aacagcggtg cttatgtttc ggaaataatg cgcggcggta tcctatccgt tgacccgggt
     5941 cagcttgagg gcggacgctc cgtcggtatg agctatgcga caacaatgtt taaaatcgtt
     6001 attcctcagg ctatcaagaa tattcttccg accctcggca acgagtttat cacgcttgtc
     6061 aaggaaacgt caatcgtttc tttcgtcgga gcaatggatt tgtataaggt gtttaacgat
     6121 atcggaacaa acaactatga atttatggtg ccgtaccttg taatggcagt catttatatt
     6181 ataattgttt gcctcatcac gcttggcatt aagtttatgg aaaggagact gagaaagagt
     6241 gatagacgta gttaagctga ataaaagttt cggcgatcat catgttctta aggatatttc
     6301 cgtaacgatt gaaaagggcg aacgagttgt tattatcgga ccgtcgggtt cgggaaaatc
     6361 aacctttctc cgttgcctta actgtatgga ggatccgacc tccggtcagg ttatctttga
     6421 gggagttaat attgcggatc tcagcgtaaa tatcaacacc tacagagagc gaataggaat
     6481 ggttttccag cactttaatc ttttcaataa caagacggtg cttgaaaata taatgctttc
     6541 tccgtatctt gttgagaaaa agaaaatgaa gaaagctaaa aaggataatt ttttccataa
     6601 gctcggaatg aaaaagcatc cgtatacgga aaatattcca agtgcaaaag agatcaaggc
     6661 gcagaaaaag gaagaggcta tggtgcttct taaaagaatc ggtcttgagg aaaaggcaaa
     6721 tgcataccct tcaacgcttt cgggcggaca gaagcaaaga attgcaatca tccgttcgct
     6781 tgcaatgcat ccggaggtta ttctctttga tgagcctacg tcggcgcttg acccggaaat
     6841 ggtcggcgag nnnnnnnnnn aaaacatgac gatggttgtt gttactcacg agatgggctt
     6901 tgccaaagag gcggcaacaa gagttatttt catggacgac ggaagaatgg ttgaggaaaa
     6961 cactcctgcc gaatttttct cgaatcccaa aacagaccgc tgtaaggaat tcctttcaaa
     7021 ggttcttgta taattgacag taaaactgcc gcttcggtta tccgaagcgg cagcttgtgt
     7081 tattattgtc atgtcaacaa aaaaaccgtc cgcactgtac ggacggttaa ttattcgcaa
     7141 tattactgga acgatgctcc gctttcctgc ttatcggcaa tgtcgttagg accgtctttc
     7201 caggcgggta cgcacttctg cgtcaaattt ccgcttgaat caaacctgaa ttgaatcttg
     7261 tatccaaggc ccttgaagct tgcactgccg tcctcgtatg actgagtctg cacgcaaaaa
     7321 acaggggagt agccctgagc ggcaattgtg tgatctgtcg taaatacaat gcaccaaaga
     7381 agcgcaacac cgactacgca catcagaaca atctgcaagg cattgaattt aaccttttcc
     7441 ctgtgcttct tgacaggcgc accgtcagcg atattctgat aagcttccgc actgtcggta
     7501 agctcaagcg tcgttgaagt ctcatcaatt atgcnnnnnn nnnnaatctt catcattgtg
     7561 ttcatcctct gctataggat tcgcttcaat gccgctaaca acagcttctt caagctcggc
     7621 aggcttatcc tcctgctcgg aaagtatatc tattgcttcg tcctcattga acttgattct
     7681 gttttcggct gaagtatctt taaggagctc atcaattctg ttgaactgct cctttttatt
     7741 ctcgtccata acaaacctcc aaatagttgt ataataatta tatacgatta tgaaattaaa
     7801 tcaagagaaa aacaaaaaaa ttaacaatct tttttgctat ttatattcta tagccatata
     7861 aaaagctgcc tcaggcctta acggttgagg gcagctttta tgatgattcg gtaatttgct
     7921 cagaagattt gtcatacctc tgaggctgat tttaagcaac aaagcatgcg aagcagtgcc
     7981 gaagtagcct gcgggaggat ttatgcttaa taaaatcaat tatttaaaac ctcgttaaaa
     8041 tattcggaag gactgactgt tccgataatt catcatctta tttccccgaa aatgtaaata
     8101 tttgtatcgt gcaaaatata actgccaaac attattttgc caataacgca acagtctcga
     8161 catgtgccgt gtggggaaac atatcgacgg gggtggcggt gagcgttcgg tagccgaggg
     8221 cggcaaagcg ttggcagtca cgggcgaggg ttgcgctgtc acaggagaca taaaccactc
     8281 tctcgggtga catctcggcg acggttttta tcaatgcttc gtcgcagccc tttctcggag
     8341 ggtcaaggat gacaatgtcg ggttttactc ctctttcaag cagagttgac gccgcttgtg
     8401 ccgcatcggc gcaaataaat tccgcattat caatgccgtt tagtcttgca ttttcttttg
     8461 cgttttcaat agcctgcgga atgatttcaa caccgattaa gtctttgaca tcctttgcca
     8521 tggtcagtcc gatcgtaccc gttccgcagt aaaggtcaac gagcgtttca ctacctgtga
     8581 gagaggcata ttctttagct ttgttgtata gaatttccgc tccgtcgtga ttgacctgat
     8641 aaaacgaaag gggagagaga ttgaatttta agccgcaaag ctcgtcggtg atgtaatcgc
     8701 tcccgaaaag cgttttacat tccttgccga gcacaacatt tgtcttatcg cggtttatat
     8761 tcagaataac gcttgccaag ccgtcaattt ccttgagctg ttcaatcaga gcttccgcat
     8821 gaggaatact gtctgaattg gcgacgatgc agaccattat ctgacctgtg acgaccgcct
     8881 tgcgaatata aatatgacga ataattcccg nnnnnnnnnn taaacgctta tctttttatc
     8941 acggatccag ttgcggaaaa tatcggttat ttccgaaaat tctttcggct gtaaaaggca
     9001 atcggcacag tcgattattc ggtgtgtcat cggcgcataa aaacctatcg tcgcaaatct
     9061 tccgtccgaa ccgacggggt actgtgcttt gttcctgtat cgctcagcag agggcgacgg
     9121 aatgatcgga gccggcgtaa tatcgagctt gccgagacgc ttaaaattgt cggcaacctg
     9181 ctgtgctttg atttcaagct ccgattcata ttttatatga gcaaagcagc atccgccgca
     9241 tttttccgca atagggcaga aattttttgc tctcatcggt gaacgctgca caagcttttt
     9301 aataatgccg acggcataag tctttttgac cttgataata tgagcaagca cgcggtcgcc
     9361 tgttaccgct ccgcgaacaa agaccgtcat gccgtcatgg tcacagacgc cgctgccctg
     9421 tacggtacag cttttgacag tcagatatat ttcatcgttt ttcttaagca taaaatcacc
     9481 gaagctattc tatcacattt cggacatgaa atcaacaaaa agggaaggga agagagctga
     9541 caatgcggtg tcgctttatt tttatccgat tcgccgtaaa ataacaactc ccctcgaaat
     9601 tcaaggggag agcagagctt tcattgatat attaaatatg tcagatctct ttaggcttga
     9661 agagaaattt aaaaataata cctgccaatg ccgcgccggc aagcggagcg acaagaaata
     9721 cccatacttg attgagagca ataccgccat ctaaaactgc cggagcaagg cttctggcag
     9781 gattgaccga ggtacctgtg agagggattc cgatgatgtg tacaaaggtg agcgtcagac
     9841 cgataacgat acctgcaacc gaggaatact tttcgcttga ggtaacaccg agaacagtca
     9901 gaacgaagac aaacgtcagg acaatttcaa caataattgc acccgtcatg gatataccga
     9961 cactgctgag ctcgccgtag ccgtttgagc ccaagccatc tgcagcagtc tccttggtaa
    10021 tgagcataag caagccgctg cctgcaaatg caccggcaac ctgagagaca atgtagccga
    10081 taaggtcgat tatgtcaagc ttgccgttga tgaacattgc gagcgaaact gccggattga
    10141 catgacagcc cgaaatatga ccgattgaat atgcaccgga tacgattgaa agaccgaacg
    10201 cgagggcgat tgagacaaca ccgagcgtgc cgttacctgt tccgccggaa agtccgccgc
    10261 tgaaaacagc cgttccgcag ccgaaaagta caagaattgc cgtgcctaaa aattctgcaa
    10321 tgtatttttt gagtctcata aaaattcctc ccattattca gcatcttagt atcaaactgt
    10381 tgcagccgat actgccgccg acagaatagc taaaaataaa tttgtaacag tttatttaat
    10441 ttcaaaatcc gagaccggca ataaatgccg ttgttgatgt cgtatcgttg tttactgctc
    10501 cggcgactaa gtagaaagcc aaaataataa cagtcagtat gattttccat ttcttatcca
    10561 gcttttctga tttccatatg gttacgataa ccatgacagg caggaaaaaa atccataaca
    10621 gtattgttcc tattgaggtc ttgcttttat tgtgcctgtt gttattaaag ctattagctg
    10681 caggtgatgt ctgaaatgcg gttgtcgtct gatgcgcgtt ttgaaccgaa ccgttgtatc
    10741 cgcaattcgg acaggcgttt gtataatatt cgtttccgca cttaggacac gtctgcggct
    10801 tttgtccgac cttaacaccg caattcgggc agaaacccga atcaaaaacc gtaccgcagt
    10861 tacggcactt gttgctgaat tccgacgctc cgcacgaatg gcagaccttc tcggtgtcat
    10921 cacactcact gttgcaatac ttgcatactt tcatttatac tcctccaagg tttaaacttc
    10981 attaagtata tcacacaata tattttcatg caacagtttg ttttgtctat acaaattcat
    11041 aaaaaattaa cttgtttata tctatatgtc gtgatataat tatatagaaa caaaagagcg
    11101 tttcaaggag gtcaaacata tggatatagc agatcttgcg gcaagcgccg tgaagaaaat
    11161 tacgaaaagc aagggtaaaa gacttgccaa aaaaggttat cccgaggatt tgccgaaggt
    11221 tcagcctgta aaaatctcta ataaaacaga cgataagttt gacgtcggct ttgcaatgaa
    11281 agagacgatg ccggacgatt ttgagtccaa gacctattac atagcgggac ataagatggc
    11341 tcagaaaatg acaggcttcc atgacccgat tacaataagc gcaatgtgga tcggctgcgg
    11401 cgatgagggc ggcgtaatga tggtctgtgc cgacgttatc ggccttacca attttgaggt
    11461 aagtataatc agagcgagcc ttgaagagtt ttcgagcaag gcaaagtgca aggcgatcaa
    11521 tgtttgctgc tcgcataccc acggcggatt tgataccgta ggctattggg gcaagctccc
    11581 gaagagcggc aaggtcgata catatatgca gaagattttt aagaccgtca aggaagtatg
    11641 ccttgaggct tatgacaatc gcaaaaaggg tgatctttat atcggaacaa ctcatgttcc
    11701 cgacgctcag tatgataagc gtcctccggt agtccttcat gatacattga caaggattcg
    11761 ctttgttccc gatgacggca gcaaggaaac gtggctcctc aactatgctg ctcatccgaa
    11821 tacactcggc ggctccaaca ctctttgcag tgccgactat ccttacttcc tcaggcagac
    11881 gatttataag gaaaaggatg tcaatgtcct ttacggtatc ggtgctatcg gcgctgtcga
    11941 cccgggctat ccggatattg aggatagatg gaagaggacc gagcttcagg gtgagactct
    12001 cggaaaggcc gctctttcaa tcgacaacga cgagaagatg aagccggaaa ttaccgttgt
    12061 tcagcagccg ttctattact atgcggataa cgctgtgctt gctttcctcg caatgctcaa
    12121 ggttatgagc tcaaagattg ttcctgcaaa gaagggcgat ctcggaattg ctctcatttc
    12181 cgaaatgaca tacatgaaaa tcggttcgca gcagattctc cttatgcccg gcgaagtttt
    12241 ccccgaggtt gcttacggcg gatatgattc ggcagaggat tcggcaacgg gcagaggtcc
    12301 cgaaattgat ccgannnnnn nnnnccgctc gttgacatcg cagaagataa ggatttgctt
    12361 atcttcggtg taacaaatga tatgacgggc tacgttgtcg ctccgaatga tttcattctc
    12421 cacaagacgc agccgtatct ttcaagcggc agagaccgtt ttgacagaaa tcactatcat
    12481 gagactaact caatgggata ttctgcggcg cagacgctta ccgatgtgtt tgccgatatt
    12541 atgaagagag ttaaataaaa gcatttacct ccccttgtca agcgagggga ggcgattttt
    12601 gagagaagat gaataacgga aaaattctcg gtgcagtggg tattgcaccc tacgataagt
    12661 gtgataagag aataatccgt aggggtcgat gcccacatcg acccaaggcc agaagggact
    12721 attcccgtgc gtgggttcct ccatcgctga agccgaccga gggagtaaga tctcatcaaa
    12781 gcagaaacgc ttattgaaat taactttttt gtagttgcgt gcattacacg ttaccaaggt
    12841 cagaagaaaa taaaagcctg ataattaccg attaaagaag gaaacccgat gaaatacaaa
    12901 tacgagttgc attgccatac tgccgaaacg agcgaatgtg cggcggcctt ggcaaaggac
    12961 acggttgaat tctacaagtg aataggttac agtggtcttg tgattacgga tcattattcg
    13021 tttctgactt tcggaacgga cagtgctttt aaaaggcaga tcgacgttga caaatatctc
    13081 aaaggttatc actgtgccct tgaagccgca ggcgatgact tcacggttct gctcggaatg
    13141 gagataagat attttgcaac gacgaacgac tatctcgttt acggaattga tgaggatttt
    13201 ctaagaaaaa acggtaatat gcttttcaaa ggtccgcgca gattttataa gcttgtaaag
    13261 gagagcggag cgataatcgt tcaggcgcat ccgttccgac cgtatattca cagagcaaac
    13321 ccgaagtata ttgacggatg tgagattttc aacggcaagg ataaggacaa ggatcttaac
    13381 caaaaggcgc aggcatgggc gaagaaggag aaatttcaaa tcgtgacagg cggtgcggac
    13441 tatcaccgtg aaagccagag gggaaatgta tcgggaatca ttacggagga aaagataaac
    13501 acaaacgatg atcttgtcag aattttgaga aacggcagat atgaaataaa catgggatag
    13561 gcaaccgtcg gcgagtgtgt cggcggtttt gcttaattgt aaatttttta caaattgggc
    13621 aaaatgtatt tatttttaaa atataatatg atattatatc agcagagatt tatatgtatg
    13681 aaatggggaa agcatgactt gaaatgccgg aataaaataa tagcagcatt gctcacggtt
    13741 gtcgtcgctc tttcatcaat gccgtccatg gcaaaggcgg agaaaaaagc aaccggtgtt
    13801 tatcagtgcg attggtttga cgagagcctt tattatcctt atgaatacga tgatgaatgg
    13861 ttcacgggga actcttttga atataatcac aagcttgccc tgctcgcctt gaatgtctcg
    13921 atggcaacct ttaattcctt caacacaagc gacacggacg agcatattga agccatgctc
    13981 aaaaactgcg gctatgaaac taaggcatat ggctatgaga ccgagggcta cgatacagcg
    14041 gcggtcgaga tggcaaaaaa aaccgttacg ctttcgggcg agaaatgcac gatcgtcatt
    14101 gctgcaataa gaagcggaaa ctatggcatg gagtggggcg gaaatcttag agtcggcaac
    14161 ggtgacaatc accttggatt tgatatcggc aaggagatta ttctgaacta tatcaacgat
    14221 tatttcacaa cggaaaagct tgagggcaga gttaagcttc tgattcccgg ctacagcagg
    14281 ggcggctcaa tagcgaacct tgtcggcgga gagcttgacg acggaagcta tacgaagtgc
    14341 ctcaaaaatg ccgacagcat aaagacggtc ggaattgcaa agaatgacat ttatgtttac
    14401 acctttgagg cgccgcagtg cacaaaaaat gacggcgttg acggtgctgc atacggcaat
    14461 attatgaata taatgaaccc gaacgactat gttcccaagt tcgttatgaa agactgggga
    14521 ttcacacgct atggcgtgga gtattatttg ccgtcggctg aaaactgtgc gaattattcg
    14581 agctattacg aaaatgtttg caagacattt gatacattga tggaggatac cggtaaaaaa
    14641 tcaagctcga atttctacag cgaggaggac tcccgctcgg tcggcgctat gttagattca
    14701 ctgatgtcga ggcttgcaaa ggacattttt gtaagtcaag aaaattatgc cgaaaaatat
    14761 gaggacggcc ttgtatttat agcgggtcag tatatcggaa aaaaactcaa tgcaggcaat
    14821 gcccttaaaa ccctcggagt aattctgtcg gcttttgcgc tcggaattat tccgaccaat
    14881 atggatacga tcaagagcga cggcttccgc gcttatattg cttcgcagat tgcggaaagc
    14941 gatgcaagcc gaaatctcac tcaaaatcag atacagggta taattgatgt tatcatagaa
    15001 atacttgaat ttgcaaagga taaccgttcg gaggtcaggg ctttgttggg tcagctcaac
    15061 acagtgttga atgttcatca gccgtatgtg acactttcgt ggatgcgctc tgtaaatcaa
    15121 aacgatatgt tgaaaataaa cggtgagtcc gagaaacctc tgaaggtttc ttttaacaga
    15181 attgacttgc gatataaagc gaatgcaaga ataattgccg attatgacaa gactctcggc
    15241 tcactcatat ggaaatcgga ttctaacgga gttgtttccg tcgacaggga cggatttgta
    15301 acggcaaagg gtgacggaga ggcgatagta acnnnnnnnn nncacatgaa caagctggag
    15361 atcatagttt caaccgtaaa gaatatattc ggaaaggcgg cggcttaatt gagcagatat
    15421 tttgttctga tttttcttgt tctcggcata ggtgtgggtg ctttgctctc gcttgtgacg
    15481 gacgtaaata tatttgtcaa catgattatc ggtgctcttg caggcgccgc agtcggatat
    15541 ttccttaaag ttaaggttga aaaggataac ggttcggacg atataaaaga ataaaatatg
    15601 ccttttctca gttcaaaatt gggaaaaggc ttttattttt aaaatatata gtgtataata
    15661 tctatgacaa tttccaaagg agatttttta tgaaaaatat tcttgttacc ggcggagccg
    15721 gatttatcgg cagcaatttc atttattata tgcagcgcaa gcaccccgac tacagattgg
    15781 tatgtattga cgctctgacc tatgcaggca atctttatac tcttgatgcg gcacgcaagg
    15841 gtaattttcg atttgtcctc ggagatataa ctgacagaaa gactgttttt gcactctgcg
    15901 aggaggaaaa attcgatgca atagtcaatt ttgcggcnnn nnnnnnnatt tttaattctc
    15961 atgaagactt atctcgtaac cggtgccgcc ggttttatcg gtgccaagta tattaaatat
    16021 atcctggcaa aacataatga tattaaagtg gtgatactgg atgccttgac ctacgcaggc
    16081 aatctgggca ccattgccaa tgacattgac gacgaacgtt gcttttttgt aaaaggtgac
    16141 atctgcgacc gcgtgcttgc cgacgaactg tttgccaagt ataaattcga ttacgtagtg
    16201 aattttgctg ccgagagcca cgtagaccgc tcaatcgaaa atcccgaggt cttcctcaaa
    16261 acaaatattc tcggcaccca ggttttgctt gatgcctgca acaagttcga tatcccccgc
    16321 tatcatcagg tatcgacaga cgaggtttac ggcgatcttc cgcttgaccg tcccgatctt
    16381 ttctttaccg agacaacacc gatccacact tcttcaccgt attcggcaag caaggcgtcg
    16441 gctgatctgc tcgtaatggc ttatcaccga acattcggaa ctcccgcaac gatttcccgt
    16501 tgctccaaca attacggacc gtatcagttc cccgagaagc ttataccgct catgatttca
    16561 aaggctcttg ccgacgaaaa gctccccgtt tacggcgagg gactcaatgt ccgtgactgg
    16621 ctctatgttg acgaccattg taaggcgatc gacatgatcc ttggaaacgg caagatcggc
    16681 gaggtatata atatcggcgg tcataatgaa aaagcgaata ttgatgttgt taaaacaatt
    16741 cttaaagcag tcggcaagga tgaaagtctt attacttatg ttaaggatcg cccgggtcac
    16801 gacatgagat atgccattga tccggcaaaa attcatgccg agctcggctg gacacccgag
    16861 actttgttca atgagggaat tcagaaaact atcgagtggt atctcaacaa ccgtgaatgg
    16921 tggcagaaga tactcagtgg cgaatacaag aactataggg gatgatttta tgaaaagaat
    16981 tgttgctgtt ctgcttactg ccgttattgc gataacgttc tttgcctgcg gcaagaaaga
    17041 gggcgacctc ggcaaacaga caactgttga caagaaaaac ggctatactc tcgttgaaga
    17101 agttctgacc tttaagtata atgacgatga ggaagaaatc aagaaaaatc ccaatgcaaa
    17161 aaccgaggga tttgttacaa atgagagtaa tgcagtcggc aaggtcaaca caaaatccgc
    17221 cgtccttgac atcgcaaaga aagaagtcgg cgaaaaattc aacaaagtca attttgcctt
    17281 tgaccgcacg cagggaattt ggagagtaac attctccatg gacaccgtga ccgatgaaaa
    17341 tacaacaagc aagaaagtta tgaccgttta cattgatgag gacggttata ccttggcaac
    17401 aattaaggca taaataaacg gcggtgaaag tgccgccgtt tttttaaaag gagtgctcaa
    17461 aatggcattt gaaagaagac atgagctgtg gacttttgat atattcccaa aggttatggc
    17521 ggcaggcaaa gaaaaaaaca tacatatccg gccgtttggt gacagattat tttttacagt
    17581 cggcaacgag tataccgcag ttttgacttg gcttaacggc ggctccgagg atcaatatcc
    17641 ggctaccgga tatcgccgca gaattcccgt tacggcaaca gagaacggaa ttgatataaa
    17701 agtgacattt ccttatgagg gcgaatatcg cattaagttc accaacgaag aagatgacaa
    17761 tttaaccttt gccgtctatg ctgttgacgg cgatttgaca gatgtttatc cgttcatcgg
    17821 cgatcttcat atgcacacca actattccga cggcgatcac gaccctgcga atgttgttgc
    17881 aagctaccgt gcacacggct atgatttcct ttcgataacc gaccacagac gctactatcc
    17941 gtcgctttat gcgattgagc agtttaagaa tattccgacc gaaatgaatc tggttatggg
    18001 cgaggaggtt catctccctc cgataaaggg cttcagagtt tgtcctcata caataaactt
    18061 tggcggcgaa tacagcatta actcccttgt tgaggatgag gcagtcgaag aagtcggcaa
    18121 ggacaaaaag tttcgtgcta cccgtgaaga ttgtcccgag gttatgaccc gtgatgagtt
    18181 tgaggacaaa atgaccgagc ttgcaaagga tttcaaggtt cccgataacg ttgatccgct
    18241 tgtcgcttca actcttaaat ggatttatga cgagataaga aaggcgaacg gacttgcaat
    18301 ttttgtccac ccgacttgga taacgggcaa caccttccat gattccgatg cactcaacga
    18361 ttggcttgtc gagaacaaaa tatttgacgc attcgaggtg ctcggcggcg aaaactattt
    18421 tgaacagaac ggatatcaga ccgtacgtta ttacgaggat aaagcaagag gctacagata
    18481 ccccgttgtc ggcagtaccg acagtcacaa ctgcacgccc gaaaacagga acgcttatat
    18541 ttgctcgaca atcgtttttt cacctgaaaa cgaacgcagg gcaataattg attcgataaa
    18601 gaatttccga agcgttgccg ttgatacaat aagtaaggaa ttccgccttg taggtgaaat
    18661 gagatatgtc cgctacggct gcttcctgct caagaactat ttcccgattc atgacgatgc
    18721 atgcttcgag gaaggcagaa tgatgaagca ggcaatatac ggcacagacg acgaaaagca
    18781 aacggcgatt ggtatgctca aacttatgaa tggcagaatg aagaaaatga gagaaaaata
    18841 tttttcattc taattaaaca gaacaaaaca cagctcccga cttgaacaaa tcgggagctg
    18901 tgtttttaat ttatgatgaa ttggcaactt agtttaataa agtctgaaga atggagtgta
    18961 atgacaatca agtatagtgt agaaaacaaa gaagtctacc acatggaatg gcaaaaatca
    19021 aataaagaaa aacgcggaga aaagcaacac gcggaggagt tggagg
//
LOCUS       DAWBSW010000015        18580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_664_length_18580_cov_2.709690, whole genome shotgun sequence.
ACCESSION   DAWBSW010000015 DAWBSW010000000
VERSION     DAWBSW010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 18580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18580
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_664_length_18580_cov_2.709690"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6899..6908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12141..12236
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13182..13191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5136 a         4028 c         4282 g         5018 t
ORIGIN      
        1 atatggattt acaatagtaa agactgaaaa cggtgtttca aggtatgcag accctaaaaa
       61 aggaaaagat gctgccgagc tttcagaaat gcttaatgcg tgggctgaac aatccgagaa
      121 atatcacaac ggcgacataa atcgtgacga atacgataag tggcgctaca attatccaaa
      181 gtatgatgaa acttccagct ttgtaaaagt accgtcacag aatctcagcg atgctatggt
      241 tgaagctttc aaagataggt tgaagaacga ttaaaaatga taaaacaaaa agagctatca
      301 gactttcaaa aaatctgata gctctttact tttacaataa ttcaaataat gttgccattt
      361 tgttgccaca cgggacactc aaatggctaa atccgcataa ctacgggctt tttcacgggt
      421 gtgaaattat tcccactcca ccgttccggg aggtttggag gtgatgtcgt agacgatgcg
      481 gttgacgccg tcaacctcgt tggtgatgcg gttggagacg gttgcaagga tatcatacgg
      541 gattcttgcc cagtctgcgg tcatgaagtc gtccgttgtg acggcacgga tcgcgatgac
      601 attctcgtac gttctgtgat cgcccataac gccgaccgac tttgagcctg tgtaaacgca
      661 gaagaactgg ctgaggttct tttcatagcc gttctttgcc atctcatctc tgagaacaaa
      721 gtctgcgtcc tggagtattc ttatcttttc ggctgttata tcgccgacaa ttcttacgcc
      781 gagacccgga cccgggaacg gctggcgcca tacaagctca tggggaattc cgagcatctc
      841 accgaccttg cgaacctcgt ccttaaaaag atccttgagc ggttcaatcg tgccgaggaa
      901 ctgtatatcc gacggctttt caaccatgtt gtgatgagtt ttgataacgg ccgtgtttgc
      961 cttatccttg ctcttgccct taccggactc gattctgtcg ggataaatcg taccctgtgc
     1021 gaaaaatccg tcctcagcct gttttctgac ctcgtcccag aataccttat agaattccgt
     1081 accgataatt tttctctttt gctcggggtc aacaacgccc ttgagcatcg caaggaagtg
     1141 gtcgccgcag tttacgcgga caaaattcat atcaaagagg gaagtgaagg tctgctcaac
     1201 aaaatctccc tcatcttttc tcataagacc ctgatcgaca aaaacgcagg tgagttgctt
     1261 gccgacggca cggctgagaa gtgcagcggc aacagagctg tcaacaccgc ccgaaagacc
     1321 gagaacgacc tttttatcgc cgatcttttc acggagcttt tcaacggttg tatcaatgaa
     1381 gctgtccatc ttccagtcac ctgtgcagcc gcaaacattg aaaacaaagt ttctgagcat
     1441 ttgattgccg tactcggaat gagtaacctc ggggtggaat tgaacagcat agagctttct
     1501 gtcaacgttc tgcattgcag cgcaggggca gttgtcagtt ttggcgataa tctcaaagcc
     1561 cttgggcggt tcggagatat aatccgtatg gctcatccaa acaacactct tttcgggaac
     1621 gttctcccaa agaaggctgt ccttgctcag ctgaagctca atcttgccgt attcggaaac
     1681 aggagcggtt gcaaccttgc cgttgcccat ccatgcgatg agctggcatc cgtagcagat
     1741 gccgagaata ggaacgccga tgtcaagaat atcttttgta taatgcggag attccatatc
     1801 gaaaacagag ttcggtccgc cggtgaagat gattcccttg tagccgtttg ccttgatcgt
     1861 ctcaaggtcg gtggtgtagg ggagaatttc ggcgtaaacg ttgaaatcac ggactctgcg
     1921 ggcgatgagc tgattgtact gtccgccgaa atcgaggaca agtatttttt cgttcattct
     1981 tttcgtcctt tctaaaaatg gtgaggggtt gttgaattca gatgcagaaa gcgttttctt
     2041 tgccccgaag ctgtacttgt gtactgcgag agggcaaaaa agcgcttaaa agagcataaa
     2101 aattttagat aacatcaatt aaatcaaagc tataatccac atggcttatc ttggctactg
     2161 tatgtgaatt atagcttttt atttttgctc tttggtctgc gctgaatgcg gcaacacctt
     2221 tttatcggta tataatttct tctctcttcg gaccgttgga aaccattgta atcggcttgc
     2281 cgatctcctt ttcgataaag aggacatagt cctgtgcttc cttcgggagc ttgtcgaatt
     2341 ctttgatgcc tcttatgtcg cacttgaagc cgggaagtgt ttcgaaaatc ggttttgctt
     2401 tcttgagcat atgagtgttc gggaaatcct taatgatttt tccgtcaatc tcatagccgg
     2461 tgcaaacctt gatttcatca agatagctga gacagtcgag agcggtcagg caaacctgag
     2521 ttgcgccctg aacctcaaca ccgtagcgtg ttgcaacgca gtcgaaccag cccatacgac
     2581 ggggacgacc tgttgttgcg ccgtattcgc cggcatcgcc gccgtgatta cgcattttat
     2641 cggcttcctc gccgaaaatc tcgctgacga attcgcctgc gccgactgcc gaggaataag
     2701 cctttgtaac cgtgattata tccttgattt catgtgccgg aatacctgcg cctacagtgc
     2761 cgtagcctgc gagagttgag gaggatgtaa ccatcgggta gataccgaaa tcgggatctt
     2821 tgagtgagcc gagctgaccc tcaagaagaa tttccttgcc gtccttgatt gcctgacgaa
     2881 taaatgcgcc cgtgtcggca acataaggct caatcatgtc cttgtactcc ataagtgtgt
     2941 tgaaaagctc gtcaacatca aggagaggtt tatgataaac gtacttaaga aggaggttct
     3001 taacctcgca aacgcttgca agcttttcct taagttcatc ggggtagaaa agatcgcata
     3061 cctggaaacc gatcttagca tacttgtccg aatagaacgg agcgatacca gattttgtcg
     3121 agccgaactg cttgtctgcg agacgctctt cctcgtatgt gtcgagaaga acgtgataag
     3181 gcatcataat ctgtgccctg tcggaaacaa gaagcttggg ctgaggaacg cccttggaag
     3241 taacgtcgtt aatttcctta acgagcttag gaatatcaag agctacgccg ttaccgatga
     3301 tgtttgtaat ctcgggatgg catatgcccg acggaagaag atgcagagca aactttccgt
     3361 aatcgttgat aattgtgtga cctgcgtttg caccgccctg gaaacggata acgacatccg
     3421 ctttggaagc aaacatatct gtgattttac ccttaccttc gtcgccccag ttagcgccga
     3481 caattgctct taccaatgaa agcacctcca aatgaaatat ggaactctcg ttccaaacat
     3541 gataattata atatttttaa gaagtaatgt caatgagaat aaggaagttt ttcgtggcaa
     3601 tcgacggaaa aagaagaaaa cagtcgttcg ggtatgattg acgacggtga caattgatga
     3661 tataatcaaa gcggtgatta aattgagcat gagaaagcag ataaaattta ataacggaca
     3721 ccttaaggtt ttgcagattt ccgatttgca ggataatcgc ttcacttgcg ttgacacact
     3781 tcgcttcatg gaagcagcgg tgaaaaaaat aaagcccgat cttatagttt tcacgggcga
     3841 tcagcttgat gtcgttgacc tttggggcaa aggggagagg tgccgaagga atgttgaaaa
     3901 ggcgatcaac cgtcttttca gtgtttttga aagttttgat attccttttg tgctgacctt
     3961 cggcaatcac gaccgtgaaa cgggacttcc aaacgatgaa caggcgaaaa tctatgcaaa
     4021 gatgaaaaac tgtatctgtt ttgacgatgt gaacgacggc agacccgatg caggaacgtt
     4081 taatgtacca atcatgtcct cagacggaag ccgcacggct atgaatattt atgtttttga
     4141 cacaggttca aaaaccaacg gtgtttacgg cggcgtcaga aaggaacagc ttgaatggct
     4201 tgacaaaacg agccgtgagg tcaatgcgga ttcaatcgtt tttcagcata ttccggttga
     4261 cgaaatatat gagctgctcg aaaaggttcc gaagggaacg gagggtgccg agcctgccta
     4321 cggaaacaga aaaggcgaat attacaggcg cagggacggc attaaattca tgggtgagta
     4381 cggcgaaaca cccgcggctc tgccccgtga gtgcggcgaa tttgaacagc tgaaaaagca
     4441 gggagatgtt tttgccgtct attgcggcca tgaccattat gacagcttta tcggaacggt
     4501 tgacggaatt gacctcggct attgccccgg tgcaggctat aacacctatg gcatatatca
     4561 aagagaggtc agagtttttg aattcaacga aaacgatgta aaaaactata agacctatac
     4621 tgtgagttac ggcgatgttt gcaataagcc gctcgccgaa cctgtcaaaa cctatatttt
     4681 cagcattgca ccgtgctgca ctccgcagct gccgatgttt gcggtcaagg tttttgcatt
     4741 gcttgccgca attgccgtgc tgtttgttct gctggcaaag gtcatcggat cgaaaatcgt
     4801 aatcggaata ctattggcgt tgcttgccgt ttctgtaata tatttcggcg gagcgataat
     4861 ttataatatt gttacaagaa aaaagctgat tgagaggtat cgaaatgaac gaggtaatta
     4921 aagcaattct tgaaagacag acgataagat catataaaaa agagcagata acggacgagc
     4981 agctcgacct gctcatgcag gcggcaaaga aggcgccgag cggaagaaat atgcagccct
     5041 gccatgtccg gtttattcaa aacaaagaaa tgcttgacca aatgaacact gattttaagg
     5101 agcttgtcgg ctacgataca cctgcctaca cccgttggga tgtgaatccc gtttaccaca
     5161 atgctccgac ctttgcggtt cttttcgcag agaatgagag ctatatggac ggcggaatta
     5221 tgtgtgaaaa tatctgcatt gcagcacagg gtctcggact cggaacatgt ataatcgcaa
     5281 gcgtcggtgc actctttgct gacggaaatg ccgagggtaa caagtggaaa agggcatggg
     5341 atattccgga aaattataaa tttcttattg ccgtagcaat aggatatccc gatgagaagc
     5401 ccgaggaaaa gccgcgcttc gacgaccgta taaaggtaat aagatgaagt attcgataga
     5461 cagaattgaa gaaaacatcg ccgtttgcga gggcgacgac ggcaacgttc tgaagctgaa
     5521 gcttgacgag ctgccaaagg gaacccgtga gggagacata atagagaaaa cagaaaacgg
     5581 ctttataatt gacgctgacg aaactcaatt acgccgcaaa aaaatggcgg aaatgcaaag
     5641 aaatatattc ggaaagaaaa gaaaatgaac tctaaaaggg tgattttatg aaaaaactgg
     5701 aatgccatct ttccttcgac aatacatgct gctggatgtg gacgctgtcg tctatatttg
     5761 tcggctttaa gatcttggag gaaaaaggac ttttgaaggt taaatccgtc tcgatggaca
     5821 gaaatttccg tgccgacgga cgctatcccg accgtatgat agttgaactg aaggcggacg
     5881 gaaaaacaat tgcctatgat atgtccgacg gatatcaaag catcaatatt cccgaattgt
     5941 tcgattctca gctggacagg cttgattatt attttaaatc gagctatgat ccgaattttg
     6001 ccgaaaagct caggaatcac gataaatttc ttcctctcgg aatagcttac gaatgttcat
     6061 gtgacggaaa ctattttgaa aaagcaaata ttaacgatgc gctgaaaaat cacagatata
     6121 aagagttcgc ttttcagata ttgacaaaag ccaaaaggca aaggctgttg aactacaaaa
     6181 actttgaagg caatgagcac tttgataact ataaaatcct tttttggtca cggctttgga
     6241 atgtgcatac gacgccggaa gaaattttaa aggtgtattc tgagcttgat tatgataggg
     6301 caaaggaaaa ggcagagacc cagaaccgaa tgtttgagga tgtcaacagg caaagaattc
     6361 aatgcgtgca ggttctcaaa aaggaattcg gctcgcgatt tgtcggtgga ctgagtcaca
     6421 gtgaggaaag ccgcagtctt gcccctgaac tgattactca cgatcctgct atagagacaa
     6481 gggaggaata cctcgcttca ttaaaaaaga actacataaa tgttttatca aagggcttgc
     6541 acggctgtat aggtgcgaga tacggagaga catttgctgc gggcagagca tttatgacgg
     6601 atccgcttgt gtatgcgccg gcaggaaacc cgcaaaagga tatcaattat cttgaatata
     6661 ccgatgcgta ttcattggca gaaaacatga atcgacttat taccgatgtt gacagaatac
     6721 atgaaatcga aaacgcaaat aatgagtatt acaataatta tgtcagacct gattcaagaa
     6781 tactgaacac gctgaaaatt gctttccctg aatattttta aaaagaacag aaccgcagat
     6841 ttttgagatg ctgcggttct gccgtttttt attttaattt aactgcaata tattcatcnn
     6901 nnnnnnnnta ttcatccgtt tcaacgatgc agtctttgct cgggtagggg gcgatatcag
     6961 aatacatttt gtgagcttca tcatactgtg ctcttgtgca atgcttaaag ctgacgccga
     7021 tgtaattgca gaatttgtaa agttcgtcct cgggcttgtc tctgtcaaat gagtttctta
     7081 tgctccaatc cagataggat cttccgatat aatcatattt tcgctttgct ttatcggcgg
     7141 aatcgccgga tgccaaacgg tttatgagtt tgcccaacgg agatgaatcg gaaacggagg
     7201 ttaagtctgt tatgtgatcc gacatattat agccgccgat aaagacaaca ggcttgtttg
     7261 tatcgcagtt ttcacttatt cggctgttca ctgtcaaaat aacgtttttt tcttcctcaa
     7321 atcgctggtt atttatccaa tacgatttgt ttaaagcgcc gatttgaata atcagtgtga
     7381 aaacaaataa agcggcaaag gctttcttag cgaggctttt gcctgattga acattaagac
     7441 aaagaagaaa agccgtaaaa gcaacaaaga acggcattcc ctgttcggtt ctgtattccg
     7501 cgccgccgcc tctgaaaagt gtcatagcaa aaacagggag aatcccgccg agaaaaagaa
     7561 gaaaactcga aaagcatttt gtctttttta tgttgacaat gaataaaata atgaatatta
     7621 ttcctgccgc aaacaataca gtaaaggcgg gattgcagaa tactttaagc ccccaatagg
     7681 caatatatga acggataaga tttttaacag cttttattga tgctgtccaa aagctttgag
     7741 ttcttgcaaa agaattgact tcaattgaga agatttttat gagcgccttt gtgataacag
     7801 tctcaaacac aacaccgagt acaagaactg cggcatgaaa aattcctttt gaaataacct
     7861 ctcccgtttt tattccgttt tcagtgcgtt ctttaagtat aaaaactgca aataccgtgc
     7921 cgatataggg cagaattatg cattcatacc aggaaaaaac aaacatcatc agcacagtcg
     7981 gcagaataag gcatcgccat atatcgctgt atttaatcga aaaatacaat gcaagcgaca
     8041 gcagaaccgt acagcctccg accgtcagta ttgctccgtt ataaataaaa tactccgcca
     8101 gaagaggaaa tgagatatat atgcaggaaa aaacaataga cggaacgatg cttctgtatt
     8161 ccgtgaaacc gtcaaaaatt gcgagcatta taagcgttcc gagaataaag caaacgagtc
     8221 cgagcaacgg ctcaaaccaa acggggcagt cccataaatt gagcactctt tcgacaatcg
     8281 ttgccgtaaa ccttccctga ccgaaaagca taccgctgta atatcggtca tatgaaagcc
     8341 agtcgattga cggcgaaggg ttggtaagat aaaagccgta tgctccgaga gcggtcaacg
     8401 caaccgctat cagataaaaa ggattggtta ttcgtttgca tatcgactct ttgaggtcaa
     8461 gattttgatt tgtaagctta tgtttaatca tttttatttt ccccgttatt attaaagttt
     8521 attctttctt cctcaataac aagcggccga ttcataaccc ttgtgtttat attgagaaca
     8581 tattctccga gaagtccgat aaagaatagc tgaacgcttc cgagaaagca catgccgata
     8641 agcacgggtg ccatgcccat cgggaagtta tcccacatga taagcttgag aatgagataa
     8701 attattccga caatgaagct tatcgcacct actcctacgc cgaaaaatgt tgcgagcctg
     8761 aggccgattt tggtgtaaga ggtgaagctg agcatcgccg catcataaag cgtgtaaaaa
     8821 ttattatgtg tctttcccgc tctgcgcttt gcctgctcat aaggaatatc cttgcgcctg
     8881 tagccgagct ccgcaacaat tcctctgagg aaaggcgtgg gatccttgag atttctgaga
     8941 acctcaatga atgacttgtc ataaagaccg aagccggtaa agtgttcaat ctgctcaacg
     9001 tcgctcattt tcttgatgag tttataatag caggtacgca aaaatctgat gattttattt
     9061 tccttgctcg ttgttttaat ggcgcagaca attttatagc cgttctccca ttcgtgaaca
     9121 agcttcggaa tcatttcgat cgggtcctga aaatcgcagc acatacaaat tgtgcagtcg
     9181 cctgttgttt gcagcattcc gtaataaggt gaattgaatt ggccgaagtt tttggcattg
     9241 aaaattgcct tgatcttcgg attgtttttg caaatttcac gaaggagcgg acgtgtgttg
     9301 tcctttgaat cattgtcaat aaagagaatt tcatagtcat attcgggaag actttttcct
     9361 aattcattta tgacagcctc acttatcgga acaacgtttt cttcctcgtt atagcacgga
     9421 atcataatgc tgattttttt catattcaag acctcaatcc aaaaattctt ttattcctgc
     9481 ttcaaagctt atttcgggaa caaatcccgt gtcctttgtc agctcggaaa tatccgcctg
     9541 taaaaacatg acctgattgt gagagtacgg tattgcgccg aagtcaattt ccgaccggct
     9601 gccgcagagc tcatgtattt tttctatata ttctctcagc ggtttggctt tgccgcttcc
     9661 gagacagtag attttttcat tgtgttcact gtcacagccg acgagataaa acgcgtttgc
     9721 ggcatccctt gcactcagaa aatcccagag ctgttcgccc ttgctgaatt cggccttttc
     9781 attggcggtg aatttattta ttgccgattt tattacggag ttctccggat cgtacttccc
     9841 gtaaacgctg agaattctca cccatgtgtg atttatgccg agcttgcggc attttagcct
     9901 gctgagctga cctgcggcaa gcttagccat gccgtagccc gtaaaaggaa atgccggtgt
     9961 ttttggggtc agcgacttat cgcaaagacc gtattccgcc tgcgaacctg cgccgataaa
    10021 ttttcggcag cccatttttt ttgccagctc aacggcatca agggtgtatt caatattttt
    10081 aagctgaagg tcggcgtcgt ctcttgccgt tcccgtggtg cccgcccaag cgaggtggta
    10141 gaaaacatca taatcatttt cttcgggaac aaagcttttc atttcgtcaa gagaacagca
    10201 gactttttta ctgagtctgt tgtcgggaat atcgtcaact ctttttgaac ctctgtgaca
    10261 gagcacggtg atctcgacgt tttcggcgat gagcttgtcg attattgctc ggccgaccga
    10321 gcctgttgca cctgttatta cggctttttt cataatatca ctcttttatc agcggaatgt
    10381 acatgttctg agccagttca tctctgtcaa ggaacggagc aagatcctca agcggaggac
    10441 tgacaagcgt tccgtcgggg agacgtttag tgctgctctt aggctcccaa acctgctttg
    10501 tatccgtgaa gatttcgcag aaagcatagc cgtcggttgc aagagccttt ttgaccgcgt
    10561 cctgcatttc ggcattactg cttgccgaaa aatacgggaa tccgaaggcc ttggctatat
    10621 tctcaaattt cgggaaggag agatcgccgc tctccggacc gatgcctacc ttagtatgat
    10681 ctttgaacag gttgttttga gttattctga tactgtggta gccgctgttg ttaataagga
    10741 aaagcttgat cggcagctta tttgttacta ttgtctggag ctcctgaaga ttcatcatta
    10801 tgctgccgtc accctcgagg caaatcgtgt cctgcttttt gcccgaaaca cagagaccga
    10861 cagccgccgg cagaccgtag cccatgcttg cgattgcgct gttgatgatg aagcgtgaac
    10921 cgtgcttgat tgtccagcac tgatggccgg caacgcagca agcgccgttg ctgaccgccg
    10981 taagcgaatt ttcgggaagc tgatcggcaa gatattttat gaaagcataa acatttgccg
    11041 ttgtgccgtt ctcctcctta tgacgcggga gcacgaccgg atatttttct ttccattcaa
    11101 gacactgtct gcgccaatcg tttccgccga aaacgggagc attttcaaca cttacatttc
    11161 tgataacggc ttcgagaaaa tcctttgcgt ccgcccaaac aggcatatca acatgaatgg
    11221 tcggtttttt catctcagcc ttgtcaatgt caaccatgat tacctcggcg gctcttgccc
    11281 atgtgccgta gtcatagccg acctgacgta tgctgattct tgtgccaacc gtgagaagaa
    11341 gatcggaatt ttgaacggca aagtttcccg ggcggtcgcc catgttgccg cccctgccgc
    11401 aatagagagg gtctttatcc tctataacgt caatgctgtt ccagtatgtt acgacgggaa
    11461 cgccgagaag ctttgaagct tttttgaaag cttcatatcc gcccgaaaga cgtattccgc
    11521 cgccggcata aataaccggg cgcttggcat tttttatttt tcggagaatt attttaattg
    11581 tattgtcatc gatcttgggc ggaagctcac cgtcgtcatc ggtcgggtca tagcccttga
    11641 ggtcatccgt ttcaatataa ctgccctgat aattaacggg tatatcaagc caaacgggac
    11701 ccggtctgcc cgttgtggcg agatgccatg ccttttcaag catataacgt atatcgttga
    11761 tatcctcaag catggcggag tatttgcaca tgcaggagat agcttttgtg atatcaaatt
    11821 cctggtcacc gacagctctg agagggagtc cctcggtgaa ctgctcggca tatcgtgccg
    11881 ttgtggtgta tttaacctga ccgctgatga caaacatagg aatcgaatcg agccatccgc
    11941 cgacaacgcc ggtaattgcg tttgtgccgc cgggacctgt agtaacgcag agagcggcaa
    12001 ttttgttatt gagtctggca taggcttctg ccgcaattgc acttgcctgc tcatggtgat
    12061 tataggtgca atgaattctg tcgttatgac cgaatgcgtc gttgaggtgc attgcaccgc
    12121 cgccgacaac agtaaagcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgc
    12241 aatatagtca gcaagtctga ttttcatttt tttcatcctt tgtcggtttt gatttaaaag
    12301 cccaaaattt gttcatgaca aaattgagcg gaattgttat aacgagcgcg ccgaggcttg
    12361 caatgaagaa cggaacgctg atttttgtaa gtagaatttt tattatcggc gaaagtatca
    12421 agcctgtaat accgtagcag gcaaaggtct tgaaataagt tttgaaatgc tcggaaaagc
    12481 tctttttgcc tgaaccgaaa acatatctgt tgttccaaaa atacgagttt gaaacgctga
    12541 caacaaaagc aatgatagtc gttataacga ttttaacatt gttgccgtcg gtagatatgc
    12601 cgagaagtgc aagaagattt aaaatgccga taaattttgt gttcttgaaa agaacgtagt
    12661 agcaaagcat ttcaatgccg taggaaatcg cggtgttaga cacgccgaca agcccgaatt
    12721 ttataaactg tatcagcgag ctaagctttt cgttctgcaa aagagctttg attttattca
    12781 tttttgagcg cctcgatgac agtctttgcc atatacatga gcttttcgtc cgtcatgccg
    12841 ggataaacgc cgatccaaaa ggaatcattc atgattctgt cggtgttttc gagcgtaccg
    12901 acaacacggt agcccttgcc gcttgcacgc atctcgtcaa agcagggatg ctttataaga
    12961 ttgccggcga acagcattct tgtctgaacg cccttactct caacgtattg aacgagcttg
    13021 ttcctgtcaa cgccgtccct gcaggtgatg aagaatccga accagctcgg gtcggaattc
    13081 tcattcgcct ccggaagcat gagcttatcg gaaacgcact caagagcatc gtgaagtatc
    13141 ttgaaattgt gctttctctt ttcaacgaag cttggaaact tnnnnnnnnn ncagccgaca
    13201 gccgcctgca tatccgtggc tttaagattg tagccgaagt gggagtaaac atatttatgg
    13261 tcgtagccga gcggaagctc gccgtactgt ctgtcaaatc ggtgaccgca gaggttatcc
    13321 tgaccgggct cgcacataca gtcacgtccc cagtcacgca tagagcggat aatcttgtga
    13381 agaagcggat tgtttgtata aactgcgccg ccttcgccca ttgtcatatg gtgcggcgga
    13441 tagaagctgc ttgtgccgat atctccgatt gtgcccgtaa atttcgtctc tccgtcaatt
    13501 gtataggtcg agccgagcga atcgcagtta tcttcaacaa gccaaaggtt gtgcctgtcg
    13561 cagaaagcct tgacagactt gaggtcaaac ggattgccga gcgtatgtgc aaccatgacg
    13621 gcctttgtct tatctgaata tgctttttcg agcatggaaa cgtcaatgtc tgcattcgga
    13681 agctttacat caacaaaaac gggaattgcg ccgaactgaa tcatcggtgt aacagtcgtc
    13741 gggaagcctg ccgcaacggt gattacctca tcgccgggtt tgatcgctct ttctttgagg
    13801 aggggagagg tgagcgtcat aaacgcaaca aggtttgcgc ttgagcccga attgacgagc
    13861 gaacaaaact ttacgccgag atatttgctg aaatcgctct caaactgctt tgtatatctt
    13921 cctgctgtga gccagaattc aagcgcgctg tcaacaaggt tcaccatttc ctcagagtcg
    13981 taaactcttg aagcgtaagg gattcgctgt ccctctctga attcggttgg aacattgtga
    14041 aatttgtcgc agtattccct taccttttcg agtatttcat catgtaactg ttctttttcg
    14101 ttcattgcga tttctccgtt caaatattaa aaaattcttt tatctgcttg gcggtatata
    14161 ccgtcatgtc ctcattgccg acatatgcct ttgtccattc aacggttttt tcaacagcct
    14221 gacgtgccgt ccatctcggt ttccagccga aaacagattt gacctttgaa cagtcaagct
    14281 tgaggaaatt tgcctcgtgc ggtccgtcct tatgctcagc aacgaagctc tgtccgccgc
    14341 cccaagcgtc gcagaagcag cctgccagct cgcccgttgt gagacagtcg gactcattag
    14401 ggccgacatt gtaccaatcg gcaacgcttg cgtcctcata ctgcgccgca gctatcatca
    14461 agtaaacata gagcggttca agcacatgct gatagggacg aatggagttc ggatttcgca
    14521 ccttaatatc ctcaccttta attgccgccc ttacgcagtc ggggataatt ctgtttgccg
    14581 caaagtcgcc gccgcctata acattgcctg ctcttgcggt ggaaattctt acacgattat
    14641 ctgcaaagaa tgagcttttg tagctgtggg ttacaagctc ggagcaggat tttgagtttg
    14701 aatacggatc aaagccgtca agaggctcat tctcacggaa gccccaaata gtttcatcgt
    14761 ttttataaac tttatcggtc gtaacgttga gaaacgattt gaccgaatct cccgttctta
    14821 tacattcgag aatgttgacc gtgcccatta cattggtttc ataagtatat ttcggattgt
    14881 tgtaggattc gaggacgatc ggctgagctg caagatgtaa aacaatctcg ggctgaatct
    14941 cgtcaaaaac atttttgaga ttctcaaagt ctctgatatc gccgattacg gaattcattc
    15001 ttcctccgag acccgtaatc tcaaacatac tcgggttcgt cggcggttca agcgaatagc
    15061 ctgttatatc ggcacccgca agcaggagaa tgttgcaaag ccacgttccc ttgaagcccg
    15121 tgtggcccgt aatgagaacc tttttacctt tataaaacgt aagatcaagc ataataatta
    15181 ctccgttaag ttatctttat taccagattt tccacggtgc ggtggagtct gcccacattt
    15241 tttcaagctg ctgcttttct ctgagggtgt ccatcggttt ccagaagccg tcatgcatat
    15301 aagcgtcaag ccgccgtcgt tatcgctttt ttcacggaag gaagaaatct tattgtcttt
    15361 ttcaatatca agcacaccga agcgttgacc gatattaacg gcggtcagtg ttgcaatgtg
    15421 tccgttatcc ttatgaaatt taacgaggtc gtcaatattg atgtctgcaa cgccgtcacc
    15481 gtaggtcagc atgaaaggct cgtcgcctat atatttctga actctcttaa ctctgccgcc
    15541 tgtcattgtg ttaaggcccg tatcaacaag cgtaaccttc catggctcat tctcgtttga
    15601 atgaacaatc attttgccgt tatcggtaaa gtcgaaggta atatcactcc tgtaaaggaa
    15661 ataatcggca aaatattcct tgataacatg ctgcttatag ccgcagcaga taacaaaatc
    15721 attatagccg taagatgagt agattttcat tatatgccaa aggatcggca taccgcctat
    15781 ctcaaccata ggtttaggaa taagatgact ctcctcactt attctggttc cgaatccgcc
    15841 cgcaagaata accgttttca taaaatcgcc gccttttcat tatttttaaa tacaaattcc
    15901 cagtataaaa tacctatatt actatatcac atgcaacggt taaaatcaag aatttaatac
    15961 tgtttacacg ctttgatttg aacaaaaaac tatcttatgc gctttatgtc caaaatagga
    16021 agcacgtttt tttgcacatt tcacaaaaat aaaaaatctg agggaaatct attgacattt
    16081 gctcgtgttt ggtttatcct atggtcgtag ggtaaacgat tacccaagcc aaacgaaatg
    16141 gagagtatat ggacggtaaa agcatttcga tggtcgatat agcccgaatg tcgggagttt
    16201 cgatcgccac ggtctcaaga gtccttaata agaacggacg gtattccgcc gagaccgaaa
    16261 aaaaagtaat ggcagtcgtc gagaagtaca attatgccat aaatcccaac gcacagggat
    16321 taagaaccaa caggacaaaa actatcggag ttataattcc cgatatcaca aacgaatatt
    16381 tctcacagat agtaaggtcg gttgaaagca cggttatccc gatgggatat aacgtatttg
    16441 tctgcgacag tgacgagaat gttgattatg aatctcatca tattgcgtca ctccagtcaa
    16501 agggtgttga aggtataatt tatttcagcg taagaaaacc gggcgacgaa gtttcaaagg
    16561 aatttacggt tcccgttgtt tacattgaca gacgcccggg tgttgaattt aacttcgttt
    16621 cttccgacaa cgaaacgggc ggcttcctcg caacggagga gcttatcaaa aagggctgcc
    16681 gaaagatagt tcttattcac gatgtcaact cttactcaac ggttaacaaa cgtaagcagg
    16741 gctacctcag agcacaaaag caatatggca ttgagattga tgacagcaga atggttcctt
    16801 gcagagtttc ctatcccgat gcgaggaaca cggtaaaaaa acttatcgcc gacggcgtag
    16861 actttgacgg agttttctgc acaacggatt ccatggcact cggagcactg agaggtctta
    16921 aagagtgcgg aaaaagagtt cccgaggacg tgaagctggt aggctttgac ggtataacac
    16981 ataccgagat ttcagatccg ccgataacaa cggtcattca ggacacagac cagctcggta
    17041 aaagatcggt tgaggttttg atggatctta tcaataacaa cacgggtaag attcacgaat
    17101 acacaattcc cgttaagctt tcggttagag gaagcacgga ctgattaaat aatttaagaa
    17161 agggttcagc ccttttttaa acagctgttg agtaaacctt tactcaacga tatatacaaa
    17221 ttatttccac ataatatatt tcgtaaagca gcattgagaa aggaaggata ccatgagtac
    17281 tctcgttgaa atgaaagggg tagtcaaaaa gttccccgga gttacagcgc tgaaggatat
    17341 ttccttcgat atccaagcgg gcgaagtaca cgtcctgctc ggagagaacg gagctggtaa
    17401 atcgaccctg atgaaaattc tttcgggcgt ttaccagccg accgagggaa aaataatcat
    17461 cggcggcaag gaattcagca agctcacgcc gaaggactcc tatgataacg gaatcagcgt
    17521 tatctatcag gaattgagcg ttatcaatga actttcgatt caggaaaatc tttttgtcgg
    17581 aaagcttccg atgaaaaaga taggacttgc atcggtcgtt gattacggcg aaatgaacag
    17641 gaaagcgaaa gagcttctgg caaagatcgg tcttaacaga gacccgaaaa cgttggtcga
    17701 ggatatcagc gtttcggaaa agcagcaggt cgaaattgca aaggcgctgg catccgacag
    17761 taaggtaatc gtcatggacg agcctacggc ctctctgaca aacaaagagg ttgagcacct
    17821 ttttgatgtt gtcagatcac tgaaaaagga aggcaaagga atcgtattta tctcgcataa
    17881 gcttgccgag attaaagaaa tcggagacag agtttcggtt cttaaggacg gtgcttatgt
    17941 cggaacaaag caggtaagcg atgtttcggt tgacgatctt gtaactatga tggtcggcag
    18001 aacggttcag aacacctacc agcatgagga cgctcctgat aattacgacg gcgaggaggt
    18061 aatcttcgag gtcaagaatc tttcccgtaa ggacggaaag gtaaaggacg tttcgttcaa
    18121 gcttcataag ggcgaaatag tcggtttttc cggactcgtc ggatcgggcc gaagcgaaat
    18181 gatgaactcg atcttcggtg ccgaaccgaa atcgtcgggc gaagttttca taggcggcaa
    18241 gaaggtcaac atcaaaagcc cgtattcggc aataaagcac ggacttgcga tggttacgga
    18301 aaacagacgt gagaccggat tcttccacaa ctttgatatc aagcagaata tttcgattct
    18361 gccgtttata aaagcgtcaa gtctcggcgg tgccggcgga ctgataagca gcaaatttga
    18421 aaaagaatgt gccgaagctc agaaaaagag ccttaatatc aaatgttatt ccgtagatca
    18481 gggtatcacg gagctttcgg gcggtaatca gcagaaggta atcctcggca aatgggtagc
    18541 cgccgattca cagataatta tttttgacga gcccacaaag
//
LOCUS       DAWBSW010000016        18489 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_667_length_18489_cov_3.136812, whole genome shotgun sequence.
ACCESSION   DAWBSW010000016 DAWBSW010000000
VERSION     DAWBSW010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 18489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18489
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_18489_cov_3.136812"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11642..11651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4993 a         4390 c         3784 g         5302 t
ORIGIN      
        1 gataacggat atgattgtta tgaaatttat tccgaggaca aaaaagttgt gctcgccgga
       61 aattcaccgc tgagtcaagc aatggcctat tacgactatc tcggcaagta tcacggcgtt
      121 gttataacaa gcggagatta tgatatttca acgatctcct ccgctcccct gcccgagaaa
      181 aaaatcagcc gaacaataaa gaaaaaaatc cgtgccatga caagctatga atcgttttcg
      241 ctcaacggca atttccgcgg ctttgaccgc tgggaaaaag agattgactt tatggctatg
      301 cacggcttta accgtgcgct tcagcctgtc ggctttgacg gcgtactttt cagaacgttg
      361 accgatatcg gcatgcccga gaatttctgt cttgaatttt cctccggccc tgcattttta
      421 atgaaccagc tcacgggcaa catcgccgga acaaattcag tcaacagcaa agagtatctt
      481 gagagaaaaa tcaccgtcgg caagcttata agtgaccgtg aaagaagtct cgggattgaa
      541 ccgatttttc ctgcggcaat tccgagtgtt cctttcagcc tcagaaaaaa gtatatcaag
      601 atggacattt ttaaagctcc gatgtggtac aattttcctc ctattttttt catcagaccg
      661 aagaatgctt ttttctctgt ctttaataaa aaattcctga caattcagcg tgaagcgctc
      721 ggcgagacac acagctatat ctttgagggc gtatacgaat cggataaaaa aggctacaat
      781 tctcatcttg ccgacctcgg taaagccctc gaagaaatgc tcggcgaatt tgatgcggaa
      841 gccgtctgct atctgcacac aagctcaata aatgccgact ttttcaaaaa ctgttcggga
      901 gacagataca tttttcttga caactgtgaa atgagcaaaa gctccgatat tctgaacggt
      961 cggaagtaca ttcccgagat aagcggcaac cgttacggca gaacgggcat ttacggcaat
     1021 gtgcaaaaac tttgcgacaa tccgtttgcc aattccgagc tcggcggcgc tctttcattt
     1081 gatacttttg acattaaccc ggtttattgn nnnnnnnnng ctgctttaaa agctatcact
     1141 actgacggca attttgaccg cgacgaattc atcagagatt tttgtgccaa aagatacaag
     1201 acagattctt tcaccgatga cattatttcc ctcattgacc tttgctcaag cgacgagtgt
     1261 acaggctcga taatctgcgc ccgtccgtgc acaaacgtca agcataccgc accgtacgat
     1321 acaatggaac gggactatga tttccacaag ctttatccga tcgcccaaaa tattgccagc
     1381 tccgatgaca aaaaaatcga cgcaatgcgt gccgatcttc aatcaattgt ccgtcagttt
     1441 ttgtccgacc tcgcttaccc tgtttacatt aaggcaactg aatttttcaa agagaaaaat
     1501 gtcagaaact ttgaacaggc aagcaatctt ttccttgaaa tttgcgagga catcgaccgc
     1561 cttctgagga cgagagaaga aacgaacttc tgcacaaaat atcttgaggc gcaggaactc
     1621 ggaagcagtc aggaagaaaa ggaatccttg cagataaact tccttctgct ccacacaatc
     1681 tggggaccgt tcgatcattc gattctctat gatacggttt ggaacgagtg gggcggactg
     1741 gtgaaggatt attatgaggc acgatggcat atgtactacc gttcgctcgc cgcatatttt
     1801 gacaatccga aaaagctcaa ggacaattca aaaaagcagc cgcttgaccg caacgaatac
     1861 aacggctcat atcaggcaaa acgacttgct ctttttgaaa acaacttcct cgaaaattat
     1921 attcctcgca aaaacggaat cggtgaggag gacactgtgg aagtggcaaa agaattgctt
     1981 gaaaaatatt cagaagttta tacacagttt taaggagaac ctcaatgaaa aatattccga
     2041 attcaattac cgctcacatt gacgaagtgg cgggaaaatt aaaaaaatat ccgaagcttg
     2101 aaatgcttta ccgcaactgt taccctaaca cgcttgagac aacggcggaa attcttcccg
     2161 acggttcaac atttctttac acaggcgata ttcctgcaat gtggctcaga gactccacgg
     2221 ctcaggttac gcattatctt ccgctgacta agaacgaccc cgagatccgt tcgattatcc
     2281 gaggtctcat agttcgtcag cttgcttaca taagaatcga cccttatgcc aatgcattca
     2341 acattgagaa gaacggccac ggtcacaccg acgatttgcc gctcaacgat ccgtgggttt
     2401 gggagcgtaa atatgaaatc gactcgctct gttacccgtt cagactcgca tatctctatt
     2461 ataaagagtc cggcgataag actataattg atgacgattt catcaaaacc gctaagaccg
     2521 ttgttgatct ttggataacc gaacagcgtc attttgaaaa atcgccgtac aaatttcagc
     2581 gcttcggctg tcgatggatc gacaccctcc ataacggcgg catgggctat cccgttaatt
     2641 tcaccggcat gacatggagc ggcttccgtc cgagcgacga cagttgtaca ttcggctatc
     2701 ttatcgcttc aaatatgttt gccgctgttg ttctcggata tattgatgag attttcaccg
     2761 agatgaatat tgaagatgcg gattttcttg caaaggttcg aaatcttcgt gaagaaattg
     2821 agtatggaat caatacctac ggcaccgtta ttcacccgaa atacggcagg atttttgcct
     2881 gtgaaacgga cggcttcggt aatcatgttc ttgccgatga cgctaatatt ccaagccttc
     2941 tcagcgcacc gtatctcggc tattgtgacg taaatgaccc tgtttatata aacacaagaa
     3001 gatttattct cagcgaggat aacccgttct attacacagg taaggcagct tcgggtgtag
     3061 gcagtcccca cacgccgcac ggcttcatct ggcacattgc tctttcgatg cagggcttaa
     3121 cctcaactga cccgaatgag attaaaaagc tcattgaaac gctcgtagcg acagacgcag
     3181 acacaggcta tatgcatgag ggcttcaatg ccgacgatgc gacagccttt acccgtccgt
     3241 ggtttgcatg gtcgaattca atcttcgcag agtttatcga gtctgccctt gataaaggaa
     3301 ttatataaat tcaaggcaat gccgcacact tgctgtattg ccttaatata tgcacgaata
     3361 aaccccgccg gaatttttcc gacggggaat ctttgtttaa gctataagtt acggcatgtt
     3421 tacatagtcg agcggatcga ccgtttcgcc gttatatctg acttcaaagt gaagatgcgg
     3481 acccgttgac cagccggtgc tgccgactcg ggcaataatt tgacctcttg aaacatactg
     3541 tccctgcgaa acaagaagcg cgctgcaatg gccgtagagg gaggtatatc cgtcgccgtg
     3601 gtcaattata atatagttgc catagcccgt gccgctctcg ctctcaagcg atgaaagaat
     3661 aaccgtacct gctcttgtcg caacaatgtt cttgccgtaa gctccgctca tggaaacatc
     3721 tataccgctg tggtatctcc agtcgccgtc aagcggatga taacgatagc cataatatga
     3781 tgtgaggtaa actccgtcat aaggcaatgg ccatgcccag cccgaggagc tgacgtttga
     3841 attcatactg ccggcgttag agccgcccga tgatgagccg ccgcttgaac ccgaggagct
     3901 ctggttggct tttctgatag cttccttgat ttctttgtca agtctcgaca tttcagaatt
     3961 atactcatca atcgaaaccc tgagttcctt tgtctgagca ttcagcgcat tgagctttgc
     4021 ttcaacctct tcggtcttct gcttgataac gttctgattt gccgtcaacg tgtcctccgt
     4081 ggactgcaag tcgcttcgtt ttgcttcaag ctccgtcttt tccttttgga gcgctgcctt
     4141 cttgacttca agctcctttt gcattttttc aatagaagct atttccttat tgagtttatc
     4201 aacaagatcc tgatcgctct ccgtcacacg tttgaacatt tcgagacggt taagaaagct
     4261 tgacatatcc gacgaagaag tgaaaatctc aagcaccgag gtctcgcctg ccatgtaatt
     4321 tgctctcatt ctatcacaga aaagcttaac ggtgtcttta atttcctgat cctttgcggc
     4381 aatctctgcg tcaagctttg cgatctcgga attgagcgaa gatatcttct tttcggttgc
     4441 atcaatatcc tcagttatga gatctttttg cgccgttatg ttctcacgct cctgattaag
     4501 cttttgaagc tgaacattga gcgcggagac aatttttgac tgatccgcct gctgactttc
     4561 aagctctttt actttctgct cgctctccgc caccttgtct tcaatgtcgg agtactcgcc
     4621 tctcaaatcg ctaagcgtct tcgcctgcgc ttttggcgca tacgacatga atgacacaaa
     4681 gacaagagcc gtactcataa gcagtgcaat tattttattc agtttcttca tacacaactc
     4741 ctccacgttc cttgagatac ttctgtatgg aaataagact tcctattccg ccggaaacaa
     4801 tacctatagc aaggaaagca acaagaaggt ataccgcata atcggtaaac ggtacaattg
     4861 taaagcccct gcttaccatc gtaagcattt gggtaaccgt tgactgaagc gctctgtaaa
     4921 cgccccaaac gattgccagg gagagaactc ccgaaattgc gccgagaacc ataccttcaa
     4981 ccatgaacgg ccaacggatg aaccaattcg tcgcgccgac tgatttcata atatttattt
     5041 caagtcttct gctgtacatt gtgatgcgaa cggtgttcgc aatgatgaac agcgatatta
     5101 gaagcagaac ggcaattaca ccgatgctta cataaaaaat cgtacgtctg attctgacaa
     5161 gtgtctgtgc aatatctctg ttgtctcgaa ccgagtcaac tccgtcaagc ttttgcagct
     5221 cggaaacggt actgtcaaac ttggtcatat cgctgacaga aaccttaaaa agatccggca
     5281 agggattgtc aacgccctta aaatatgttg catccgtgcc aagttcttca agctgcttct
     5341 taaatgccgt ttccttgtca acaaactcgc acttttttac attctgcata agtgtgattt
     5401 tttcctgcag ttcagttatg ccctcgccct gaacctcgtc cttgagataa accatgacaa
     5461 cgttctgact ttcaatctcg gccataacgt tgttgacatt gactataacc ataaatgccg
     5521 cacccatcat aacaaggcat gacatcaaaa cggcgattga agcaatggac ataaggtggt
     5581 taactctgat atttctgaag ccctcttttg taagatagcc taagcgtcct ctttttctca
     5641 ctgatcctca ccgtcctttt ccgaatcctg ttcaatgtaa atacggtaag aatcattgtc
     5701 gcttataata gcgtcaagga aatcctcatc aaccttttct tcttttttat cctcagtatt
     5761 tgtcttttct tcctttgaag aatatttttc ctcaatggtc tgcttttcaa ggttaaggtg
     5821 tgccgcagac tgaaccggca gctcgccgtt ttcaatcatt ccgtcggcta caacctcgcc
     5881 gctttcaagc gttatgactc gcttgtcaaa tttcttgaca aggtcatgtg catgagtaac
     5941 cattataact gttgtgcctg cctcattgag tctcgtgaga agctcaacaa tttccaacga
     6001 catctgcgga tcaacattac ctgtaggctc atcggcgata attatatctg cgttatttgc
     6061 aagcgcacgt gcaagcgcaa ctctttgctg ctcgccgccc gaaagctcat taggatagtt
     6121 tcttgccttt gcggaaagac cgacaatatt tataacatag ggaacacgtt ttctgatatc
     6181 cttctccctt gcaccgacaa ctctcatcgc aaacgcaacg ttatcgtaaa cggtcatagt
     6241 cggaataaga cggaaatcct ggaaaacaat tcccatgttt ctgcgaaaat acggaatttt
     6301 ctttttcttc atctcgttga gattatagcc cttgatgact atagttcccg atgaaggaac
     6361 ctcctcgcgc ataataagct tgaggaatgt actctttccc gctcccgatg ctccgacgac
     6421 gaaaacgaac tcacccttgt caatatgaat attaacattg tgcagagcct tggttccgtt
     6481 gtcgtaaacc ttggatacgt ttttaaaatc aatcatgtta tctgtcctta gttgtatcag
     6541 tatttgatcg gatttgcgtc aagatatctc tttaccatga gtgcgacttt gaatacgata
     6601 gcgtcgtcaa actcacgaag gtcgagaccc gtgatcttct taatcttctc aagacgataa
     6661 acaagagtat ttctgtggac aaagagcttc cttgatgtct cactgacgtt gaggttattc
     6721 tcaaagaact tctgaatcgt gaacagagtt tcctgatcga gactctcaat tgagccgcgc
     6781 ttaaatacct cacgaaggaa aagctcgcaa agcgttgtcg gcaactgata gataagacgg
     6841 gcaatgccga gactgtcata gctgacgata gttttttcgt tgtcaaatac cttgccgacc
     6901 tcaagagaga tctgcgcttc cttaaaggag cgtgcaagct ccttgatgcc tgcaaccgtt
     6961 gtgccgatac cgataacgga atgaacataa agctcggtcg aaagtgaatc ggcaattgag
     7021 cgtgcaagct tttcaagatc cttggattct atgttgctct taacctcctt gacaagtgca
     7081 atatcggtct cgttgatact tacaacgaaa tccttggact tgtccgggaa aagattctga
     7141 actatgtcat aggcggaaac atctgtctgg ttcgggattc tgataagaag aaccgcacgg
     7201 gtggcgtcgt tgttaaagcg aagctcacga gccttgagat agatatctcc gggaagaatg
     7261 ttgtcaagaa taacattctt tataaagttg tttctgtcat atttctcgtc gaaatactgc
     7321 ttgagattgc tcaaagcaac tgcgctgata ttggtatatt tctctgcaag ctcgtcatca
     7381 ccgtcaacaa aaaccgcata ctcggggtga atctgattgc cgaaggtctt gtatgtgcag
     7441 ccgtcaacaa cacatgcttc cgtgtccata aaaacgctgg ctgcagccgt gggcactacc
     7501 tcgccgattc ttccaagctc gctgcacgaa ataattgtgc ctgtttcatc aatgatacca
     7561 attgatctgt cgatcgcgct cctcatctga tgaatgactc cctgaaaaag tctgtttgac
     7621 ataaaatctc tcctcacgat tgtttttcac atcatacttc gcatttcaaa tggttttagg
     7681 catgtttata caaaatgcca acctaaacat atacatttta cacaatttca aagaatattt
     7741 caaccctttt aagcatattt tttgaaaaat cttttcaaaa cagacgtaaa tcaacggata
     7801 ttttataaat aatgcccaaa gtattccttt ttttgccgtc attatgactt gtgtaaaatc
     7861 accaaatatt gatttaaaac gtcaaaaaca tacccctgta tacaatattt tggaaaactg
     7921 ttgtcatttt caccaatggc aaaaggagtc cgcgactttc ggtcggaacc ccttttgttg
     7981 ttataaatta ttcaaaaagc gaactgcatc aatatccgct accgaagaac tggccccaat
     8041 cgtatccgcc ctgagcctgg ctctcggttg ttgtctcagt ctcggacgag ttacccttgt
     8101 cctctacaag tgtcgcttca acggtgaatg tatcaaccga gtagtctgaa ttgatcctgc
     8161 aaagctcaag cttgagctta tctccaacct tgccgttttc aatcttatca agaagaacat
     8221 cggctgtgtc aagcttctcg ccgtcaaccg ccgtgataag atcgccgacc tttgccttgc
     8281 tgtttgcaag tgagctgtcg gaattgatcg aagcgataac aagagaaccg ctcggtaaat
     8341 tcttgagctt aactaccata gcatactgct gatatttttc tgctgcggca taggagatgc
     8401 cgagcttcgg tctgtctgta acatagccgt tggcgatgat atcgtcaata atttgcttag
     8461 cgtcggagat cggaattgcg aatgccattc cctcgtatcc ggtgcttaca atctttgaag
     8521 agttaatacc gataacctga ccgtattcat tcataagagc gccgcccgag ttacccgagt
     8581 tgataggcgt tgtgtgctga atacatttca tctcgtagcc gctctcgctg ctgataggtc
     8641 tgtcgatagc ggaaaccatg ccctttgtaa aagaaccgaa gaattcggag ccgcccgggt
     8701 taccgatagc ataaacagtc tgtccctttt tcagggttgt cgaatcgccg aactcagcgg
     8761 cggtaagacc tgttttggca atcttgataa cgccgacatc ggtctttgaa tcataaccga
     8821 caagaatgcc gtcatagttg ttgccctcgt agtccgtaac cgtcatcgtg tagcccgagg
     8881 tcttaacgga gctgataaca tgggcacagg ttacaatgta ggtgtatttc tttgagctgt
     8941 cctcgcccat gataataccc gagccttcgc cgacaagcga attttttgcg gaagacgaga
     9001 atatagactg tgactgaccg taaagtttaa taccgacatt gaatttttca gcctttgcgg
     9061 caacctcttc gctggacgga acacccgttg tattattctt tgccgtaggt gtttcggaaa
     9121 gcgttagctg agtcgagtcg cttgatactt ttgaagtatc cgccttctta accatcggat
     9181 aaactccgag aataacggca acgcaaacaa caactgcgac cagtgcgatt acaaagccct
     9241 taaagccctt gctttttttc ttctttggaa cctcgtttac ggtctttgcg tactgctgac
     9301 tgtaataatc attctgcgaa gctcccgtgc cgttgtttga gccgtagctc tgcgaattgt
     9361 acgggctgta cggtgactct ttgggtgtat catccacggt gtcattgtaa cggctgaaat
     9421 cgttataatt gttattatag ctctgctcat tgtttcggct cggctctgca tatgcctttt
     9481 ccgtttcatt ctgctgagca tatccgtttt cgtcagtccg gttgttttca gcggcggact
     9541 cggcaggctc cggagttcct tctgttccgt aatatctgtt ttcgtcactc ataataattc
     9601 ctcctgcgct gtttatagga acttttttgc tttctgttcc ttatgttgtc tacaagtata
     9661 agcccgggtt tttacgacgg tttttataaa ttgtaaacaa aaattaaacg tttggaagcc
     9721 agaatacaaa ttgtgtgtat tcattcagct tgcttttgca gacgatttga ccaccgtgaa
     9781 gctctacaat gctctttaca aggtaaagac tgagaccggc gcccttgaca tcatagcttc
     9841 tggacttatc aatcttataa aatctttcaa atattttgtc cgcttcctca cgttcaatac
     9901 ctgcaccgct gtttttgatg ctgacaaaaa gcttttcctt gtcatgatag gtcttgaact
     9961 ctatatatcc gttctgcggc gtgaacttga ccgcattatc aataaggtta tagataacct
    10021 gcatcatcat gtcctcatcg gcgctgacaa tcatactttc catcttgtca aggccgacaa
    10081 tttcaatatc tttatcgttg atctttcttt caaatgacag gagcgttttg aaaatatctg
    10141 cgctgatgtc aaactgtttc aaatttatct gcatttcacc cgcttcaagc ttggacatat
    10201 tcagcatacc tgtaacaagc cttgaaagac gcttaacctc atctgaaaca attcgcaggt
    10261 aataatcata cttgtcttcg cttatggtgc cgtcgagcat tccgtcgatg aatccgccga
    10321 tcgacgtcat cggagttttc agctcatgcg aaacatttgc aacaaagctt cttcgcgagc
    10381 tttcaagtgc agaaagcgac attgccattg tgttaaacgc ctcggaaagc tgcgccacct
    10441 cgtcatgtcc ccgaactctg accttatagc tgaaatctcc ctgagaataa tgctcggtcg
    10501 cagttgccat atccctcagc ggacgtgtaa gcctgtagct gagataataa acggcaagga
    10561 acgataaaac caacgagaac aacgcagcga caagaaagat ctgaagcatc cttttggcat
    10621 agccgtacca gcttgaagtt atcttctcgg tcgctataac gattgcaacg gttttattgt
    10681 taattctaat cggcgatgcg gcggtaatct gagttcttac aaaggttgtt cccagtctgc
    10741 cgacctccgt atagtcctta tcggttgcag cgtctataat tgattgatct atcttatatc
    10801 cgccgtgtac aacgcaggct gcctttgaat caaacctaaa gcggtcgctc atcatttctt
    10861 tgcaaacgac aacctcgccg ttcatattga taataaaaat atccgcatca agcgccgccg
    10921 acatattatt gatagtgttg caaatcattg aaagcggaga gatgtcaacc tcctctccgc
    10981 tgttgtattt ccctgaaaac tggctgagaa ttttctccga gtttttggaa atgatcgttg
    11041 tgtttcgttc aagtgtcgaa agctgctcat cattgcgata tttggaagca attccgagat
    11101 acgttgagca gactatcagc acgctgaaaa gactgactaa ggttataata gaaagatatt
    11161 tgtaaaacag ttttttgttt ttgggttctt ttcttaatct tctgcccatg gatcatcagt
    11221 ccttggtttc aaatttatat ccgacgctcc aaacagtctt gatttcccat ttgtcggaaa
    11281 cgccctcaag tttttcacgg atgcgtttaa catgaacgtc aaccgttctg ctgtcgccgt
    11341 aatagtcgaa gccccagacc tcgtcaagaa gctgatctct tgtgaaaacc ctgttcgggt
    11401 tgcttgcgag atgatagaga agctcaagct ctttcggcgg agcatcaacc ttttcgccct
    11461 ttactctcaa ttcataattg gtgagattga ttgaaagatt gtcgtaggaa acctgcttga
    11521 tattgttctc ggcttcggga gcggaagcgt tcgacgttct gcgaagaacc gctttgattc
    11581 ttgcaagaac ctctttagcc tcaaacggct tggtgatgta atcatcggca ccgagctcaa
    11641 gnnnnnnnnn nttatcaaaa gtttcacctt tggcagtaag cataattatc ggtttgttgg
    11701 aatatttcct gatttcacgg caaacctgcc agccgtcaag ctcgggcagc ataatatcaa
    11761 gaagcatgag gtcgggctga tgctcggcaa agagtttaac cgcctgcata cctgtgtttg
    11821 cgattgtaac gctgtagccc tctttttcaa gatagagtct gagaagctca catatgttgg
    11881 tgtcatcatc gactacgaga attttttctg ttgccatagt aattcctccg tttcaataaa
    11941 accctttaag gcaacacaag aaatgtgcgg cgttgcctta atgttgtctt tatcttaatt
    12001 atacttttta atttgtccat tttgtgaact aatcaaaaat attctttaaa atacatttat
    12061 ttcaaacgtc gtttttgact tgtattcctc gcctgccctg aacgtgcact gcgggaaatc
    12121 ggggtgattc ggcgtgtcgg ggtaatactg cgtttcaagg cagaagcctg catgctgaac
    12181 aagcggaagt ccgcccttgc cgatgaccga accgtcaagg aagttgcccg tatagagctg
    12241 aacacccggg aggtcggtat aaaccttaag ctgtctgccg ctgacaggct cataaacaac
    12301 tgcgtccgct tcgccctcaa catggtcaag gcagaagttg tggtcatagc ctctgcacat
    12361 tacgagctga gcatctcttt cgtgaatcct cttgccgatt gaggtcggat ttgtaaaatc
    12421 aaacggcgtg ttgaaaaccg aacggagctg acctgtcggt atactgtcct catcggtcgg
    12481 tgtaaatctc ttcgcattga gtttgagtac atgaccgagc acattgctca ttgccgaacc
    12541 gctgagatta aagtatgtat gattggtgaa gttaataacc gtatccttgt cgctgactgc
    12601 gctgtattcg atcacaacct tgttgtcctc ggtgagagta tatttcacct tggcgtccat
    12661 cttgccctca aagccgcttg ctccgtcggg gctcaagtaa gaaagctcaa cggcgttatc
    12721 gcctactgcc tctgcgtccc aaaccgcatc ggaaaactcg cctgcgctgt ggagacatgt
    12781 cctgcctttt tcatttcggg tgagttgaac ttttttgccg ttcatcacga aggaaccgtt
    12841 tgctattctg ttggcatatc tgccgacagt tttaccctga tagtccgtaa aattaagatg
    12901 accgtaaagg ctgtcaaagc cgactgtgat atcgttaact ctgccgtatt tatcgggagt
    12961 gtataacgcc tgcagcgttg caccgagcgt aatcacctga agaataattc cgtttgaatt
    13021 ttttaccgtg aaaagcctga cttcttttcc gtcgggcata tttccgaaaa aagaaacatt
    13081 aactgacata aataaaatct cctatcaatt tgttacattg tatccgacgg cgaaagaaaa
    13141 acatgaagag gaagccactg ctgaacattc gcaggaaaag cctctctgtc cgccgaaaga
    13201 tttgagaaaa atgctcttgt ggcggcatgg tgactcagtt catattccgc cttgccgtca
    13261 aatttttcga ccttaacttt attgtcaagc acctctatgc gaagcttttc gtcgccgtat
    13321 ttgccatgga caagtacggt aagcacaccg tcgcaaagct ttgagtactt agtttttgca
    13381 ccgagataag ctctgataac cttttcaaaa ttatagataa gaatcatctc acagccttca
    13441 acattgaagc cgtcagaatt ctctgtaaag aatttaatct tctcagtctc aaacggagca
    13501 acgacaaatc cgacttgaaa cgcttccgtc ttttccataa ccgcggcaac aagattcgga
    13561 taatatttcg ggttaataac accgaattca ttgacgttac tctttgtata atccatcaca
    13621 aagtatccga caaaaacgcc gttatctttg agaatatatg ggcaatccct ccacgaacag
    13681 agaacgtcaa agcagctctc cggacttctg tttgacctta tcgtaagctt cgagtaaatc
    13741 ttttcgatat tctcaatact ttcggtatct ttagaatcaa gcttttcaac ctcaagtccg
    13801 cagggcatgg ctttaagtga atgtctgtag gcacttcggc tgaaattgaa acggtaggaa
    13861 gttcccgagg actcaaatcc gaaatagccg taacgctgtc gctgaccgcc gagataagcg
    13921 acgtcaacac cgttttttct catatcttca accgaaagct ccatgagctc gatcatatag
    13981 cccattgatc tgtaatcttt tccgacagca acattgccga tgcagcaagc tttaatttgc
    14041 tcatcaccga ccgtcatgtc attataaaag ctgccgattg cggaacggaa attgccgctc
    14101 tcgtccttga caacaaaatt attgtacgcc ggcctgtatt tatccttata gcattttgga
    14161 agcaggctga gaaaatctct tccttcgggg tcgtctgtga aaaaaacttc attgagaaaa
    14221 gcaatcagtc tttcgttttc ctcatcattt cccctgccgt aatataggtt gtttgccata
    14281 gctttatatc ctttcgattt taaatcaata tccgcttgct tcctcgtttt taacaagctt
    14341 gactattctg cttgtgccga gcctgtccgc gccgagtttt ataaaatcct cggcatcggc
    14401 aagagaagca attccgccgg ccgctttaat ttttgtatcg cccgtaacgt attttgcaaa
    14461 aagggcaacg tcttctctcg ttgctccggc ggttgaaaag ccggtcgatg tttttatgaa
    14521 atctgctccc gattcggata caatctcaca catctttttc ttttcgtcat cagtcagcag
    14581 gcaggtctca ataataacct tgagaagctt gccgcggcag gcagccttga ccgcatttat
    14641 ttcattaagg gtaaaatcat actttttatc tttaagggcg ccgatattta tcaccatgtc
    14701 aatttcttcc gcaccgtttt ttaccgcgtc ctctgcttca aagcatttaa ccgcagttgt
    14761 ggaatatccg ttcgggaaac cgataaccgt gcatacagga atttttccgt caacatattc
    14821 tgcggcatcc ttaacatagc tcgccggaat gcaaaccgat gcgcagccgt attttatgcc
    14881 gtcgtcgcaa atctgcttga tttcctccca cgttgccgtc tgagaaagca aggtgtggtc
    14941 aacctttgaa agtatactct taatatccat attttttcct ttcgttatga aactacctca
    15001 cccgtctgag cgttgacaag attctggaac agcgccacct gagtggcaga aacgaacttg
    15061 tcctcgtgta agctgccgaa aaaccagcac ttaaaatcac agttcgcact cagctcctga
    15121 agataggcat tgagacccga aaccgtaagc ggttcattat ctttcagccg cagaaaattc
    15181 ttaatcttct gcgaaggttc atgagtaatc atataatcga ccttatattt gcagcgttca
    15241 agattctccg cgccctctcg catttcctct tgagtcggaa tctctgctct tgaccatgtg
    15301 tcgtggtcaa ctctcatgtc tatatcgggg ctttctccgc cgcccatggc aaagtatttc
    15361 tcaccctcta tttcaaagat ctgtcccctc ataagatgat atatattgtc cctgatctta
    15421 tgcgccttgc ctccggcgaa attaacgacg ggatatctcg caatcaaatc aaaattctca
    15481 tgggttccgt caacaaaaag aattttatat ttctttttgc cgagcttgtc gagaattttt
    15541 ttctcctttg agtcgccgcg ccatataaat ccgaaatctc cgcagacaat aagcgtgtcg
    15601 ccctctttta tattcttgaa ttttttctga gaaaagagag catagtcacc gtgcatatct
    15661 ccggttacat aaaccattgt cagatcactc cccaaaatgt gataaatttt ttaaaaatca
    15721 tgtttatttt tatagcctaa tctgttataa tacttttaag gcatttccgc ccgattgagg
    15781 cgcacgcctt atgcaatcca tgattacata aaatacacga ttacacaata tataatacta
    15841 caagttcggg tgtcttgtct atgaaaaaat ttataatttt tatatgttgt acggctttaa
    15901 ttcttacctc ggcggtttta cctgcctcag ccgcagatta tacgggagat gttactcttt
    15961 attctcaaat tgacttgctt atcaaccttg acagcaatac cgttatcatt gacaaaaatg
    16021 cagatcggca agccgacccg tcatcactga cgaaaattat gaccgctctc gtcgtgctgc
    16081 agaacgaaaa ggatctcagc aaaaaaatca ctgtttccgg atcgtcactt gaaagtcttt
    16141 acggcacagg ctgcatcatg tccggacttc aggacggcga gatcatcagc gtttatgact
    16201 tgctctgctg cctgctcatt ccgagcggaa atgacgcagc ccttgttctc gcaaacgagt
    16261 acggcaaggg cataccgaat ttcgttaaaa aaatgaacga cacagccaaa aagctcggct
    16321 gtacaaagac aaaattcacc aaccctcacg gacttgacga catcaatcag aaatccacgg
    16381 caaacgacat tgcaaaaatc acacaggcgg caatgaaata ttccgtcttt gagacgatag
    16441 tcaagatgct gacctatgac cttcaaaaga caaacaaaaa cgaggaacgc acgatagtca
    16501 ataccaacta catgctcaac tacggatttc ccgattacta ctatgaaaac gcaaaaggaa
    16561 taaaaaccgg ttccagcaat gccgaaggcg aaagcattgt atccgtagcc tcaagggacg
    16621 gttacagcta catggcaatt gccctcggcg gaccttataa ggacaccgac ggtgataatg
    16681 atactgaaaa tcaagcgatt ctcgacgcag tcagaatgtt tgaatgggca tttgacagcc
    16741 tcagctatga aatcgtcaca aaggaagcac agttcgtcac cacggttccg gtcaactatt
    16801 gctgggatat ggacagtgtc agactggttg caaagaatga agttcttgcc cttgtcccgg
    16861 agggcaacgg ttccgaaagc gtaagctttg aaccgattga tatgccggaa gctcttgatg
    16921 cgccgtttaa aaagggcgat acggtctgca aagccaaaat aatgttcgcc gaacgtcaaa
    16981 taggaacagt cgagcttgtt gtcgccgaaa atgctaacat gagcatgctt ctctatctta
    17041 aagcggcact caaaaggatg acggcaagca cggtatttaa aatattggtt gtagtagttg
    17101 ttttggtaat cggtgcatat gttgcacttt acatccgtgc gaaccgcaga cggaaaaaga
    17161 atcgggaatt aaaaatgttt aaatacagtg agcttcacca aaaccccaaa accaaggata
    17221 aaaaataaaa aaataaaacg cagagagatt cttttcgact ttctctgcgt tttttcgacc
    17281 tgccgtatat ccgacatgcc taaaattaaa agttttttgt ttttttagtt atttattttc
    17341 acctttttgt tgacatttta atttttcggt attataatat agacgttcta taaattcaca
    17401 ataataacag gtgggaatat gaatattgaa tatcaactaa caaaaactta tatttatttg
    17461 gagaatcagc agttctgcac atatggcata gatgccatca acagatcaac cgctaaaatc
    17521 caaagtcaga tttccgatgt ttctcttgac aaatcttttg ttcagcaagt catagacctg
    17581 ttgaattcgg ctgaggttga actgtgccat tttgccgaag tcgtggaaga tgagctgaat
    17641 aagtaaaaga aacaatgaat actttctgct gccgtgaaaa tctttcaccc ggccaattgt
    17701 tttatactct gtttttgccg catagcgtca agataaatat acagaatctc tgccgaaaat
    17761 agcttccgac ggattctcag ttagttcttt ctttgcaacc atacaaatat aaagaaaaac
    17821 agaccatgaa atcatggtct gtttttttgg cagcggaata gggattcgaa ccccaacaaa
    17881 cagagtcaga gtctgtcgtg ctaccgttac acaattccgc taaagaacgt ttgatattat
    17941 acatcagcaa acaggttttg tcaatatgtt ttgcagaaaa atttgaaaaa ttcctaaccc
    18001 attgcaacat ccaacagcat cataagtgca aatccgactg ccgtgcctgc tgttcctact
    18061 ctgcttttcg ccttgggaat cagctcattt gacacgacat atatcattgc tcccgccgca
    18121 aaggaaagaa catacggcaa tatcggggca acaagccaag tcagcaacac cgtcaagaca
    18181 gacgcaacag gctcgactgc tcccgaaagc acgccgtaaa gaaacgctct cttttttccc
    18241 attccgtctg taaccagcgg tgcggatata attgttccct cggggaaatt ctgcacagca
    18301 atgccgattg aaagagtcag agcttccgac agcggtataa ttccgcttat taacccggcg
    18361 aacgccacgc cgaccgccat tccctcaggt atgttatgca aggtcacggc aataaaaagc
    18421 ataatatttt cgccgagctt cggctctttc cccgtgttta aatcaaaatc gggcacaagt
    18481 gcatccaaa
//
LOCUS       DAWBSW010000017        18154 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_685_length_18154_cov_2.683297, whole genome shotgun sequence.
ACCESSION   DAWBSW010000017 DAWBSW010000000
VERSION     DAWBSW010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 18154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18154
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_18154_cov_2.683297"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1560..1611
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2667..2676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6194..6203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8433..8442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14319..14328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15746..15810
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17524..17533
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5018 a         3924 c         4473 g         4572 t
ORIGIN      
        1 agattatgct tccggttatc cccgagccga gaaaagagct ttcaaagtat gctccgaaga
       61 tgctttcaac caaggttccc gttgacaaga ttgccgaggt tatcggtaag cagggtaagg
      121 ttatccagaa gatttgcgca gagtgcgatt gtaaggttga tgttgaggag gacggcagcg
      181 tatttgtttg ttctcaggat attgagaatg caaagcgtgc tatcttcatg atcgagacta
      241 tcgccaatga tcccgaggtc ggcgcaattt acaagggcgt tgtaacaaga attatggact
      301 tcggcgcatt cgttgaaatt gcaccgggca aggaaggcct tgttcatatc agccatcttg
      361 atgtcaagag agttgacaag gtaacagacg ttgtcaacgt aggcgacgag gttatcgtta
      421 aggttcgtga gattgacgac cagggaagaa tcaatctttc gcgccgtgag gctcttatcg
      481 aggttgaagg tcttgttccg gagaatgaga tttccgacgc tccgagacgt ccgtcaggtg
      541 accgccgcag aggtggcttt agaggcggaa aaagagagca caaataatca ataagcaata
      601 attgaggctg tatcatggct catcttgagc tatggtgcag tctttttttg tcagtaaaat
      661 gacgtggtct tcttttcgtg gagcgacaga agctgaggac gtggtcgcaa gataaaagta
      721 acgagcacaa ttcgcagggg tcgatgtgtg cttgacccaa ggtcagctta tcatttattc
      781 tgcactgaaa gcgattatat taaacttttt tggtgcgatg tgggcatcgc accctacaat
      841 aagcacgaaa atcgacactt catagagaac gataggagca caattcgtag gggtcgatgt
      901 ccacatcgac ccaaggcaag aaaaagctgg ttctttcata acagtaaaat atgtaacgtg
      961 atttttttgc acaaagtata aaaaatactg gattttattt gaaattagtg ctataatatt
     1021 tttgctaaac ataatttcat atttttacca cttgtatggt ttgcgaatta tcctttcgga
     1081 taaaaatgca agctcttttt ctgcatacca tgtgagtgga taacggaatt atgtttagcg
     1141 acagttgagc tatgcgtttt ttgtgcgtgg cttcggctgc gcacttttta ttttatggag
     1201 taattataaa atgagctttt cagaaaactt taagaaagca agaaggaaca gcggactttc
     1261 acagccgcag aaagaaaaag ttgtgacttt ttatatcagt aaaatatgta acgtgattta
     1321 cttgcacaaa aaataaaaaa tactggattt ttttaaatat aaatgatata ataattatgc
     1381 gaatcaaaag atacattaaa acctaaaata ttgtccccca atgagcggta tttttattcg
     1441 ataaaaatac atgctctgaa tccatttgct cgttggggtt aattggtatc ttttgtttcg
     1501 cgacaatcgg agccgtgtgt tttttgtgcg tggcttaggg tgcgtacttt ttattttatn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaaaaaata
     1621 tgtgagattt ttggtgttac gatagacagt ctgctcaaat aacgggaaat aaagcagagc
     1681 tttctcagtg caagaagaaa aagttccgct tttttgattt aagcattgat ttttgtgtcg
     1741 atttgtggta actttgtttt atacaattta taaactttgt ccggagggtt tgaaatgaaa
     1801 aagtttatga aaaaatcagt tacaataata cttgcctgta tcatggcttt cagcgcagtg
     1861 acgtgtttcg ccgctgacgg aaaacagtat tatgattatg gtacatacgt ccttttggga
     1921 gacagcgttg ccagcggaca caatgatctt gtttacgttg attcggaatt taagcgtgtc
     1981 gaggattcct acggcgcata tgttgccgat gagcttgggg tagagtacat tcccatggct
     2041 tgtccgggca caagaacgat tgatatgcgt tatatgcttg aggacgatta tccggcggac
     2101 gattatcttt tccatgacgc tcacaatgct gaggttatga aagcaagaat ccccgaatat
     2161 cgtaaggcga tttccgaagc gagacttatc actctcggcg taggcggcaa cgatttcggc
     2221 acttttctta cgtgggttat tgccgatgag cttgaaaaaa atgactcgtg cgcagaatat
     2281 gttaaagcct tgagagatct catggagcag ggcggagccg atgacgatgt gcttgccaaa
     2341 cttgataaag caattgagct tgcaaaggtt atgggagcac tccccgagct tatgcgtgtt
     2401 ctgccgaagg caataaaata cggtatagaa aatttttcga aaaactggaa tatagttatt
     2461 gaggatattc ttgctctcaa tccggatgtt aagcttcttg ttatcggcat gtttgataac
     2521 ggcattaaaa acgaagagga cgccgctgca agcatggcag gtacaacggt tccgagtatc
     2581 ggtcagattg ttgttgatgc agcgaacact ccgatgaagg agggcgcaag gaaatacggc
     2641 tacaccttcg tttacacgac aggtacnnnn nnnnnnatat tgccgacaga gtgcttgagg
     2701 cacttcccga cgcaagattc ccgtacacag atgttgcgaa ggattcggaa tactacagcg
     2761 atgttgagtt tatgtatact cacggctata tgaacggact taccgatacg gaattcggcc
     2821 ctgatgaagc tttcacaaag gctgcacttg cacaggcact ttataatatc gcaggaacac
     2881 ctgaggttga cacagggaat gtagccttct cggatattga cagctccgac ccgacattca
     2941 atgcggcagc ttgggcaacg agtaacggag tgctgagcgc gaagaacggc aaattcgctc
     3001 ccgacagcgg gataactgta tttaattttg tattgagcct cgtcaagctt tcgtatgcag
     3061 gtaatttcgt acttgccaag gtaataaaaa caatggtttt ggcattcaat attatcaagg
     3121 atttcggttt tgcaagcttt aatacaacgc tctccagagc gcaggcggca catatgcttg
     3181 tcgattattg cggaatataa ttgaataatt ataaaaagct gtgacagttt tttgtcgcag
     3241 cttttaaaat gagtaacaca aaaaccgctg ttcgggatat ttcctaaaca gcggtttgtt
     3301 atgttaaatt atcttttaac cctgtgcgta agcgccgacc tgcatcatga agtcgccgat
     3361 ctgtgcgccg aactcgggag caccgtccat gatgagcttg ccgtgctttg tggattcaag
     3421 catatcgtcc gttgaaagga ggataccgaa tgcactctca aggcaatcaa atctcatggt
     3481 gaagaaaggc atagctctct tgtatgtgcc gcggcctgct ctggtcttgc cggccttaac
     3541 acgaaggaaa gcgttgacct cgggatgtcc gtcaaccgcc catgcataaa cacggtcggg
     3601 gctcttcttt gcccaatcat gaacagcttc gtgtccgttc ttgttgagct ggctgatgcc
     3661 cgatgaaagg agatagaaga agcacttgca ggcgagaacc tgtgttgcct cgtcctccgg
     3721 gcaagctttg atgttaagaa gcgatgacat cttgagaaga acagccataa acgctgcgaa
     3781 ttcaacgggc tttttaagaa cacccttcat cttcgggagg gttgccggag caatcttgcc
     3841 cttaaagaaa gcattcatag cttccattga cttgaactca agctcaatat gagcgtttgt
     3901 agcaacttta tccgcacaaa ctttccattc gccgttgttg actgtaaagt gcgttgcata
     3961 cttgccgcaa ggggcatcgg gatccaaaca actgacctga tagatgcagt gaatatgctc
     4021 gaatttctta gcgagcttcg gatcgtcgtt agcaatgact ttgagcaacg gcaatgcaga
     4081 gttaaggaag attttattgg ttaacaatac atcatttgcc attatcaatc attcctttcg
     4141 gtattataca tcgtcgtccc agttgtcgtc ttcctcgaag tccttattca tagcctcacg
     4201 aacagcggca atgataccgt tgcagtcgat gccgagcatt gacatgatgt cctcatggag
     4261 accgatggga gcgaacttat cctggagacc gagacgggtg aatgcacagc ccttgcctgc
     4321 ttcagccata acctcggcaa cagcgccgcc gagaccgccg agaacagtgt gatcctcaac
     4381 agtgatgata cggcgagtat ccataactgc cttgagaact gcttccttat caatgggctt
     4441 gattgtgtgc atatcaagaa cacgaacctt gagtccgtca gcttcctcga ggaacttagc
     4501 tgcctggtaa gcctggaata cacaggaacc gcaagcgata accgtgatat ctgtacccgg
     4561 gttaacctcg atagccttgc ctacctcaaa cttatactca cggggataat ccttgccgta
     4621 aagagtacgg tcgaaaccgc ggttgatacg gatataataa ggaccgaagt tctcgtaagc
     4681 tgccttaact gcttctgctg tctcgatacc gtcagcgggg cagataagag tgatgttcgg
     4741 cattgaacgg ataacagcca tatcgcagat agcgtggtga gttgaacccg cctgaccgaa
     4801 tgatgtaccc gaatgggtag caatgatctt tgcgttaacg ttctgatagc agatatccgt
     4861 atgaatctga tctgcagaac gaagagatgc gaatgttgag aaagttgaaa cgaagggaac
     4921 aagtccggcc ttagccatac ctgctgccat accgaacata ttctgctcgg caatacctac
     4981 gttgaagaaa cgatccggaa actctttacc gaatacaccg atctttgtag aacctgcaag
     5041 gtcagccgaa agaccgacga tttccttatg ctcacgaccg aggtcaacaa gtgtctggcc
     5101 gtagatttcc gctgttgaaa gctctgtgga ttcaagagct gtatatgaaa gtccgcctgc
     5161 cattattttg cctccttttc tacacgagca atacgcttct catagtgctt cttaaggctt
     5221 tccttagcct ctttatactt agactcgtca actgcgccgt aatgccagtg gtaatcgtcc
     5281 tccatgaagt cgataccttc acccttgatg gtgttggcaa taatcataac gggagcgtcc
     5341 ggctcgccga gagcgtagtc gattgcatct gaaagttcca tcatattgtg accgtcaatg
     5401 tcaaatacat ggaagccgaa tgcacgccac ttgtctgcga aaggctcaag gggcataaca
     5461 tcctctgtgc ggccgtcgat catgcacttg ttacggtcaa cgaacgagat aacgtttgtt
     5521 gcatggaatg cattgattga catagctgct tcccagtttg aaccctcgtc gcactcgccg
     5581 tcgccgagga tacagtatgt cttgtaatcc ttgccgagaa cacgggctgc aagagcagta
     5641 ccgagagcga ttgaaagtcc gtgaccgaga gaacctgttg aagcgtcaac gcctacaacc
     5701 ttgttggagt caagatgaat acccatcttt gagtttgtga ggttgaaggt cttgagctgc
     5761 tcgggatcgt tgtaaccgaa gtcacaaaga acgggagcgt aagcgattcc cgaatgaccc
     5821 tttgaaagga tgaaacggtc tctgtcttcc cagttgggat cgtccttctt gatgttgagg
     5881 tacttgtggt agagaatggt caacatatcc ataacgctca taccgccgcc gatgtggccg
     5941 ctgcctgccc agtatgtagt atcaaggcag aggttacgaa gctcaaaagc tttcttctca
     6001 agagccaacc attcctgttt ggtcaatgcc ataagagttt tcctccctat ttttattctt
     6061 ttttacacgg gcggcaagct ctgccgcccg tgattgatta tcttatctga aacttaaaaa
     6121 gcttcaggat gatccttctt aacaaccttg aatgcttcgt cggctacctc gatcgtatgc
     6181 ttgatatcgg catnnnnnnn nnnttgtggt gacctgcgaa gaaaatgcct ctcttgacgc
     6241 actctgcgat ccatgcctga tggagcatca ttgtgtcgtc gttggcaatt ctgaggtaga
     6301 agagtgacgg ctcaccggaa acaacaaggt caaatccgtt gttctttcct gcttccttaa
     6361 gaccgtcagt gagcttcttg cccttttcca taaggattga cgggaggttg agctctctca
     6421 tcttggtgat atttgcgata cctgccgcga aggggatagc gctcatccag taagaacctg
     6481 tgtaggacat tcccgaaaca gcgtccttga ggaaatcctt accgcaaagg caggatacgt
     6541 tccagccgtt ggcgatagcc ttacagaagc agatcatatc agcctcaaag ccgtagtagt
     6601 ggtcagaacc tgcaaggtca agacggaagc ctgcacgaac gtcgtcgacg ataagtacga
     6661 tgcccttctc tgtgcaaagg ttacgaacct tctgccagta gccgtctgcc ggaagctggt
     6721 tgtcaaaata gttaccgtgg aggtaaggag ttgagatgaa tgcggcaatt tcgccgtcat
     6781 gctcattaac gagcttctca agagcgtcaa agtcgttcca gtcaacatag aggttgttgg
     6841 caacgtcctc ggggagaatg cccggatagt cgatcttctg aacaactgcg gaaacaccgt
     6901 gatagaagcc gttaacgaag atgatcttct ttctgtgggt tgcgttacgg gcagccatga
     6961 tagcaagggt cgtaacgtcg ttaccgttct ttgcgaagaa agcccagtcg gctgtgttga
     7021 ctgtgtcaac cataagctct gcaagctcaa ccatcttgta ggacggagaa gttacgcagt
     7081 tgccgatctt agcctgctca agagcagcct tgtcaacgtc gggatcgccg taaccgagaa
     7141 tgttcgggcc gtatgcgcac atatagtcga tgaacttgtt gccgtcaaca tcccagaagt
     7201 atgtaccctt agccttctcg gagaagaagg gccatgcgct gacgggaatg aagttacctt
     7261 cagccgggcc gagatgaccg taaacacctg aagggattac ttttatagca cgatcgaacc
     7321 actcatggct cttctcgtac tgatttattt taaaattagc catttttcgt atccccctta
     7381 tgcaagatag agaccgactc tgtcaagaat tcggttcata ttatcgagca tgtttatcat
     7441 gccgttcatt gtgatggtac cctcgccgat cttagccatg gcgcttacct taccgttgaa
     7501 gaggtcgttt gcaagctgca ttgaaccgaa ctccatgacg gcacgcggat cctcgcaagc
     7561 cttcttgatt gtaacaaggt gaccgttctt ggcgtggata gtagccttcg ggccgccctt
     7621 aattgacatc atgatgtcgc cgtcaacgat atgctcagcg gagaacttgc cgatctcgtc
     7681 gtggttaccg atctgagaaa ttgcaacggt gatggtgtag aacataaggg ttgtgctcat
     7741 tctgaagaat tcctcatcct tgaggtcctc ctcgcttgca cgcatgtagt tcgaaagcat
     7801 gtcggtgagc ttcgtaaatg tgttcaaaag gaactttatg tgaaggaaac cttttgaggg
     7861 aataggtgta acagttccgt cgatgagacc gttaaacttc tcacatgatg agaacggaag
     7921 cttaacatcg cagtctgtgc agccctgttc catacggcat ctgccgtttt tgaatgtaag
     7981 tgttgcagac gggccgtcct taacttcaaa accgatggaa atcggcttaa tgtcagctac
     8041 aagggcgctt gccttcgggt caagctcgca caggttttca agtgtgccga gcacggcata
     8101 catattgatg tatgcaagtg tttttgcatc tgtcaaggtg cattcctcct tatcgctttt
     8161 attcaaagca tattttccat tacattgtaa caaataaagt tattaaagtc aaatgaaatt
     8221 gaccaaaaaa caacaatttt ttgttaattt attatgtctt tttattgttt ttgttcaatt
     8281 atttcctata aaagaattac aaaaatgttt ttgcaagctg attttggcgt taaaataaac
     8341 aaatattatc aacaataaat tattcctgag cttcatcctg cttattttct tcggtttctt
     8401 gctcctcggc ggtgatttcg gcattatcat cgnnnnnnnn nngtatttcc ctcctcggcc
     8461 gacgtttttt cttcctcggc aacggtcggt tccccgtcaa actccgacgg agcatcgccg
     8521 agaacgataa tgccgtttgt cttttctctg ccgcagggca gaatcacggg cgaagcgaga
     8581 gaataaacgt ccgtgcacat atcctcaagt gagaaaacat ggagaccgta atcgtcaagc
     8641 ctgtcaaagt cggaagcctt ggtgacaatg ggcttagaag acttatcctt ggcctccctg
     8701 agaatttcct tacccttgtc gttcatcgca agaacatgaa tatacggcgg ttcgcccttg
     8761 tagtcgtcgg caacgaagcc catgaaagcg tgaaagacaa ttcgtctgat tcttgcgtgt
     8821 gagtaacgtt tcgtttttgc aagctgataa agctcttcaa gagagcttgc cttgagagca
     8881 gcgtcatgaa ttctgtattc gattccctcg ctgacgtcgg gtgaaagtcc gatatcctcg
     8941 gcgctgagct ggcgcataca gcaaagaata ccgaattcga gctttgaatt ggggcagggg
     9001 gcaaggttgt tttcctcttc acggtgatag atatcggcaa cggattgcgg aacatacatt
     9061 tcccactcat tacttcccga gagcatcatc tgacgtatat ccgacgcgct ggcaaagttg
     9121 tcgcttctca taagcgaatc gtgagcggcg cctatacgct ttatcgtcat cggaatcatg
     9181 tttgaaccga gcttgtccat tgcctgaaga tattcaatgc cgagggtgtt gttcggctcc
     9241 tcgagaatgt caaaaaaacg gttgccgcag actgctttaa gagcctccga acgtgccgag
     9301 gcaaagctca ggccgtattc aagattttct ttcagacgtt cggctactgc ttcgctgttg
     9361 attgcgttct tacattctat aagctcgtca atgttgccgc attcgctgcc gaagctgagc
     9421 atatctacgc agccgaggct gttgagcagt gagactccgc cgtaggcaaa gcgctgagca
     9481 aaggatgtgc tccaaagcgt cggcagttct ataacgagat cgacgccgtt tttgactgcc
     9541 atttccgctc tcgcccattt tgagatgaga gccgtttcac ctcgctgaac atagttcgag
     9601 ctcataacgg cgacgacgtg tgtccattcg ccgagttcct tgcttttttc gatttgataa
     9661 gcatgaccgt tgtggaaggg gttgtattcc gcaacaattc ctccgatttt cataaaattc
     9721 ctccataaaa acatatattg tataaaaaat gcgaaaaaac tcaatctttg tcttgaaaaa
     9781 acaaagtcac agtaatataa tataacagaa atctttttga tacaatatct tggagggaaa
     9841 aattttgaag attctcgttg ttaatgcagg aagctcatca ctcaagtatc agcttattga
     9901 tatggaaaac gagtccgttc ttgcaaaagg aaactgcgaa cagatcggaa ccgaaagcac
     9961 atttacgcat aaggtgccgt cagacgaaac caaaaacatc aaggctttgc cgatggagat
    10021 gcaggatcat gccgcagctc ttaagattgt tcttgacgct cttgttgacg ccgagcacgg
    10081 cgttatcggc tcggttaaag agattgacgc ggtaggtcac cgtgttcttc acggcggcga
    10141 aaagttcagc ggctcggttc tcgtagacga taaggttgag gaggctatca aggagtgttt
    10201 cgacctcgga ccgcttcata acccggcaaa ccttacgggt atcaaggcat gtcagaaaat
    10261 catgcccggc gttcctcagg tagccgtgtt cgatacaggc ttccatcaga caatgcccga
    10321 ctatgcatat atgtatgctc ttccgtatga gtactatgag aagtacggta tccgccgtta
    10381 cggcttccac ggcacatcac accgcttcgt aagcaaaaaa tgcattgagc ttctcggcaa
    10441 ccccgagcac agcaagatcg taacctgcca ccttggcaac ggttcttcaa tttccgcagt
    10501 tgtcgacggc aagtgcttcg atacaacaat gggcgtaact cctcttgagg gtatcatgat
    10561 gggcacacgc tgcggctcta tcgacccggc tattgttccg attataatga aaaaagagaa
    10621 tctcactccc gacgagatgg acaccgtgat gaacaagaag tccggtatgc tcggtctttg
    10681 cggcacaagt gacaactgaa ctattgagtc gagagcgaaa gagggggaca agagagcaaa
    10741 gcttatcgag tccatgctct gtcaccagct caccaagtac ataggcggct atgctgcggc
    10801 aatgggagga cttgatgcta tcgtattcac aggcggtatc ggcgagaaca acccgcagta
    10861 cagagaccgt gtatgtgaca agctcaagtt tatgggtgtt gagatcaact atgaaatcaa
    10921 cgacaagctc aagagaggtc ccgagggcga gatctcaact ccgaactcca aggttaaggt
    10981 atatgttatc tgtaccaatg aggagcttat gatcgcaaga gacaccaagg agatcgtcga
    11041 agctctttaa tatgaaaata ttgatagatg cggacggctg tcccgttgtt gatctgacgg
    11101 taaggttcgc tgagaaatac ggcattgagt gcacaattct atgcgacaca tcgcacgaat
    11161 tcaaccgtga gggagcgaaa accgtcgtag tcgaaaaggg tgcggacagc gttgatttca
    11221 gaatagtcaa tcttgtcggc gagggcgata tagtcgttac tcaggattac ggacttgccg
    11281 ccatgtgtct tgcaaggaag gcgatacccg tcagccagaa cggaatggta tatacggata
    11341 agaatatcga tcaacttttg ttcacacgct atgtttccaa gaagataaga aaagccggcg
    11401 gcagaatgaa aggtccgtct aagcgcactc ccgaacagga caaagcattt gcagcggctc
    11461 tcgaaaagct cattcaagca taaaataaaa cctgcctcag ctatggggca ggttgatttt
    11521 tttcggtcat gcctttttag gctttttttc tgctttttat aaatccgatt gcaaggctga
    11581 taacgagcat caccaaactt acgctgaccc agcagaagcc cattgaataa agaggaagct
    11641 tttgacagag cgaggtgatg aagccgagtg aaagaccggc ttcgtcgcag gcataaataa
    11701 cgctgataac acctgtgccg ccgataacga gcggataaac ataacggttc ttcttccaaa
    11761 gcctgtcgct gagtccgagg ataatgagca cgatagccat tgggtagatc gcattaagaa
    11821 tcggaatcga aatgctgaga attgccgtca aaccgaggtt gcagaccaag aaagaaaaag
    11881 cgatgatgat gcaaacccat ttgcgatagc ttatcttttt gaaaattacc gagaaaaact
    11941 gcgaaatgga attgatgaga ccgacgcagg ttgtaaggca cgcaagtgta aaaatagcgg
    12001 cgaggagaat cgcacccggt gcgccgaaaa gctgatatac gatacagcga agcgtccatg
    12061 cgccgttttc ctgaatagga taaacgcctg agcttgccgc gcccatgtag gagagcatta
    12121 tgtaaacagc ggcaagaatc gagcctgcga ttatacctgc gaaaacagtg tgacgaacaa
    12181 ggtctttttt ctctttaagt ccgaatgtgc cgagcgtggt ggcgattaca aggccgaagt
    12241 tgagggcggc gatggtgtcc attgtctgat atccctcgga aaagcctttg aaaaatgcgc
    12301 ctttttgata cacttcctga gccggagcga tattgacctc gcccttgaat aaaaagctta
    12361 taaaaaggaa aacgataaga atgagcaggc tcggtgtcag cacatggccg attctgttga
    12421 caagcttgcc gggtgtcatg caaagccaaa gagcaatcag gaaaaacaca agcgaataaa
    12481 cagccatcca cagctttacg gactgtccct cggggaggta tggagcaaca gccatttcaa
    12541 acggaacaga tgccgccctc ggtattccga gtccgggtcc gatcgagaga taaatcaaaa
    12601 cggtaaagat aagcgaaaaa tgcttaccca cattttgagc caagcggtca agtccgtcaa
    12661 attcggcaac aacgataaca cccaataccg ggagaataac cgccgtggcg agaaagccga
    12721 ccatagccga aaccaaatga tcacctgcat tttgaccgag aaacggtgga aagataaggt
    12781 tacctgcgcc gaaaaagagt gaaaacagca tgaagcttat caatgtcatt tttctttttg
    12841 ttagtttcat tttatcctat cctttaacat tataatttat gcagaaatgt tgtctcgggg
    12901 cataaaaaaa ccgtcccaaa aatctttgag acgaatataa actgcacaaa atacccgtaa
    12961 aatgcccttc aatcaataat tatatcattt taatatggga gtgtcaatag aatcagaatg
    13021 ttaataaaga aattaaggaa aaaatatgta tttctattga caaatgcgtt tgacgggact
    13081 ataatataga aaaatttgaa ggttggagga agcttgatgt taaacaagaa aattaacaat
    13141 cgattttatt attaccatca ctgcggcctt tctgcctttg caaaaatcgg ctaaaatgat
    13201 ggctttttgc cgttgccgaa aaataaaaga gtagagattc cgcatagaag attcgttgag
    13261 aatatgctat gcggattttt tattgtcatt atcatctaag cgagggatat tcaaatgaac
    13321 aatttgcaaa atgcacgaag aacaatcgaa aagatcgacg ccgaaatggc agagcttttt
    13381 gaaaagagaa tgaaggcggc tgaggtcgtt gcggagttca aaaaagaaaa cgcactgccg
    13441 attgaggata aaaagcgtga agcggagctg accgcaaaga acagcgagtt tgtttcattt
    13501 gaatataagc cctattacga aagctttata cagaatgtta tggacgtctc aaagaaatat
    13561 cagaagcggg tgcttgaggg catgaaaatt gcctacagcg gagttgaagg cgcatttgcc
    13621 catatcgccg cgaggagaat ttttaagtgc ggcgagaccg tttcctgccc cgattttgac
    13681 agcgcatata aagccgttga ggacggagac tgcgcctgtg ccgttctgcc gatcgagaac
    13741 agctatgcgg gcgatgttga ccgtgtgctc gatcttgctt tccacgggaa tctcaccgtt
    13801 tccggcattt acgatatgcc gctcggtcag agcctgctcg caaagccggg tacggatatt
    13861 gacgatatca ccgaggtaag aagccatccg caggcactct ctcagtgtat gccttatctc
    13921 aagcgacacg gctggaagct cattcaggct gtcaacaccg ccgttgccgc caagtcggtg
    13981 gccgagggcg aaagaaacga tattgccgtc gttgcttcaa aggacacggc ggagctttac
    14041 ggattgaaaa ttttacagga ggacatcaac gagaacaaga acaacacaac gaggtttgcc
    14101 gttttcacaa gagccgaatg taaggtatca ccgaaggatg agcattttct tataatgttc
    14161 acaacgaaaa atgagccggg ttctctcggc ggagctattg ccgttatcgg cgaatacaga
    14221 ttcaacctca gaagtctcaa aagcagaccg acgggcaacg agaattggga atattatttt
    14281 tacgccgagg gcgagggtaa catcgactcc gtcgacggnn nnnnnnnnaa aatgatacat
    14341 gatcttaaaa agctttgcga gaatgtaaaa atcatcggaa gcttctcgga cgaaaaggta
    14401 ctcagcgaaa ggagcgcaga ataatgagcg tattgcacat ggagcttgga gcgaacagct
    14461 acgatataac ggtcgagcgc ggcgcactcc aaagagcagg cgagcttttg aagctcggca
    14521 gaaaggtttt gatagtaacg gatgacggcg taccgttcag gtattccgac aaaatcgcct
    14581 ccttttgcaa agagccttat gttgtgactc tgccgcaggg cgagcggaac aagtgtatgt
    14641 caacatacat gcgactgctt gaaaaaatgg tcgaaaataa tttcacaaga aatgactgcg
    14701 ttgtagccgt aggcggcgga gttatgggcg atcttgcagg ttttgtcgcc gcgtcataca
    14761 tgaggggcgt cgatttctac aatgttccga caacgctcct ttctcagatc gactcctcaa
    14821 ttggcggcaa ggttgcggtt gatttcgcgg gatacaaaaa ccttgttggc gcgttctatc
    14881 agccgaaagc cgtgctgatt gatcccgatg ttttaagcac gctcccgtca aggcagcttg
    14941 cctgcggtat ggccgaggca gtcaagatgt ttgcaacctt caatgcagag atgttctgcg
    15001 aggtcgagga aaactacgac gaaaaaattg acagaataat tattgaagct ttgaaaatca
    15061 aaaaggctgt agttgaggag gacgagaagg aaaaaggact tcgcaaggtt ctcaatttcg
    15121 gccacacggt cggtcatgcg atcgagagca tcgaagagag caacgattat ccgcttttcc
    15181 acggcgaatg tgtcggactc ggaatgcttt gcatgagcag tcccgatatt gtcaaaaggc
    15241 ttcgccgtat tcttcacaga atggatattc ctacctcatg gagaggctat gagaaggagc
    15301 ttaaagcagc tatgcttcac gataaaaagg caggcggcga cacaataacg gttataaccg
    15361 ttgataaaat aggaactttt aaagaagaaa aaatgaccgt tgacgaaatc gtagcaaggt
    15421 gcagggaggt gctttcactg tcatgaagaa cacattcgga aacgctttga ccgttacgct
    15481 tttcggcgag agccacggaa gcgcaatcgg agcggtgata gacggaattg cccccggaat
    15541 taaaatcgac acggattata tagagacgaa aatggcgctt cgcagaccaa gcggaaagat
    15601 ttccacagct cgaagcgaga aggacggact tatttttgag agcggagttt acgacggtca
    15661 cacgatgggc acgccgattt gcattttgat aaaaaatgcc gatacgcaca gctccgatta
    15721 ttccgcactt gcaggtaagg cgagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcgaaaatg ccgaatttcc tcttattgat
    15841 tcaacacttg aagaaaaaat gaaggatgcg atcatgcagg cgagagccga gggcgacagc
    15901 gtagggggag tgctcgaaac ggcggttgtc ggaatcccga aaggagtcgg tgagccatgg
    15961 tttgatacgg tcgagggctt gctttctcat gcgcttttct cggttcctgc tgtcaagggt
    16021 gttgagttcg gcgacggttt tgcccttgcc gatatgagag gcagcgagtc gaacgacgct
    16081 tacagagttg acgaaaacgg caaggcatat acgctgacga accgtaacgg aggtatcggc
    16141 ggaggaatca cgaacggaca gccgatagtt tttcgctgtg cagtcaagcc cactccgtca
    16201 atagccaaga ggcaggagac acttgatttt ctcaaggccg aaaacacgga gattgaaatc
    16261 aagggcagac atgacccgtg catagttcac cgtgcaagag cggtcgttga tgctgtcact
    16321 gcgctgacct tggcggatat gctgaccgtc agatacggta cggactggct tgcgaggtag
    16381 aattatggaa tacggatgta taggcaaaaa gctcggacac agcttttcgg cggttattca
    16441 cgcaaagctc ggcaattatg actaccatct ttgtgagctg acggaaacag agctgaccga
    16501 gtttatgaca aagaaagatt ttaaaggtat caatgtcaca attccttata aggaaacggt
    16561 tatcccgttc cttgataata ttgacgagtc ggcgaagaaa atcggcgcag ttaatacggt
    16621 tataaataac aacggtattc tgacgggtta caacaccgat ttttacggaa tgaccgagct
    16681 tttaaaaaaa ggcagtgtcg agcttgcagg caggaaagtt tttattctcg gcacgggcgg
    16741 cacctcaaag acggcaagag ccgttgcaaa gagtctcgga gcgagtgaaa ttaacgtcgt
    16801 ttcgagaaat ccggaaaacg gcgagatatc gtacgatgag atgtacaaga cagcggacgg
    16861 tgttgaggtt attatcaaca caacgccggt cggaatgttc ccgaagcccg acgcttttgc
    16921 tgttgagccc gagaaatttc cgaacctcag cggtgcggcg gacgcagttt acaatccgct
    16981 gacaacggaa tttataagcc gtgcaagagc ctgcggagcg aacgccgtca acggacttta
    17041 tatgcttgtc gctcaggcgg caaaggcggc ggaattgttc ttcaatgaca aaacgatgac
    17101 cgaaaagacc gataaggttt tctcggagat ttataaagaa aagatcaata tcgtgcttat
    17161 cggaatgccc ggaagcggca aaaccacgat aggaaaaata gtagcaaacg ctctcggcag
    17221 ggagttttac gacacagacg gggagtttgt caagtcaaac ggagctattg ccgattattt
    17281 taattcaaac ggcgaagcag cgtttaggga taaggaaagc gagattgttt cggagctcgg
    17341 agcgaagaac ggacttgtta tcgcaacggg cggcggagcg atactcagag cggagaatgt
    17401 gaagcactta aagcaaaacg gcattgtgtt tttccttgac cgtgaggttg aaaatataat
    17461 tccgacctcg ggcagaccgc tcggaagtga ccgtgaggcg atccgaaaac gctacgggga
    17521 acgnnnnnnn nnnggggaac gctatccgat ttattgctcg gcggcggata tcaaggttga
    17581 ttccaacaga gacgtcaaca ccgttgccgc cgatatagaa aaagctttca atagcatgga
    17641 gggacaggta tgaaaattct tgtaatcaac gggccgaaca tcaatatgct cggcatcaga
    17701 gaacctgata tttacggcag agagacctat gccgatctcc ttgaaaaaat aaagaattac
    17761 gccgacaaaa agggcgttga ggttgatttc tttcaatcta accatgaggg cgatatcgtc
    17821 gataagatcc agcagtcgta taataaaatt gacggcatag taatgaatcc tgcggcatac
    17881 acgcacacaa gcgtagcaat tcccgatgca ctcaaggcag tcggcatacc gaccgttgaa
    17941 gtgcacattt cggacgtaga ttcccgtgag gatttccgac agataagcta cgtcagaccg
    18001 tgctgtgcgg cgtgtataaa gggaaagggt acggacggtt actgcgaagc aatagacgtc
    18061 ttgacggatc tttttccgca atacagccca aagctttctt gacatataat aatatagtct
    18121 gcgaaatctt tgaactgtct tgcgaaaaaa tctc
//
LOCUS       DAWBSW010000018        17960 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_696_length_17960_cov_2.768277, whole genome shotgun sequence.
ACCESSION   DAWBSW010000018 DAWBSW010000000
VERSION     DAWBSW010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 17960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17960
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_17960_cov_2.768277"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4678..4687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6104..6113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10869..10878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12306..12315
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4524 a         4422 c         4023 g         4951 t
ORIGIN      
        1 gcttggtatc ataaaagctg atcgttatca tacaatcccc tccggaatta gcataaacgg
       61 tcagctttta tttattcatt taattattct atatataagc ttttgtgcag gcggcttttt
      121 attgccgaag gtgaaagcat caaaaaattc tcgcttcgct tcttcgattt tactttcatc
      181 gtcggtataa aatatcgcca gcgtatctcc cttttcaaca cggtcccctg ttttcttttt
      241 gaggactatt cctgcgctcg aatcgatcgg gtcgcctttt ttctcacgtc ctgcgccgag
      301 gattacagac gcaagcccga ttttttctgc atctgtgtgc tcaatataac cctctcgctc
      361 ggaagatatt tcaacagtat gcttcggttg tttaaaaagt gaaaaatcgt caacaacacc
      421 tgcatttccg ccctgagcgg aaatcatttc gcaaagctta ttgagcgccg agccgtcatc
      481 tattgcgccc tttgccattg catagcaagc cttttcgtcc tcacccgtca ccatgcaaag
      541 catctgcgcc gcaagtgtga gacagacatc cgtcaagtcg ctttcgccct cacctttgag
      601 cgtttttatc gcttcgatga cctcaagcga attaccgacg gaattaccga gcgggatatc
      661 catattcgta atcactgctg ttacgcttct gcctgccgat ttgcctatag agaccatttc
      721 ctttgccagg ttttcggact cctcgaaggt tttcataaat gcaccgctgc cagttttgac
      781 gtcgagaaca attccctttg agcctgcggc aagcttttta ctcattatgc ttgaggcaat
      841 caggggtatg ctctcaacgg tcgccgtaac gtcacgcaaa gcgtacagct tcttgtccgc
      901 cggggcaagc tcgcccgtct ggcctacgat gcaaagtcct atattgttga cctgctttat
      961 aaactcgtcg ggggttaatt ctgttctgaa gttcggaatt gcttcgagct tatcgaccgt
     1021 tcctcccgtg tgtccgagtc ccctgccgct catcttggca accttgccgc cgcacgaggc
     1081 aactatcggt gcaacaatca atgaggtttt atcacccacg ccgcccgtgc tgtgcttatc
     1141 gacagtgaag ccgtcaatac cgctgaggtc gatttgatcg cccgattccg ccattgcgag
     1201 cgttaattca gacgtttctt tttccgtcat gccttgaaaa taaacagcca tgcaaaaagc
     1261 cgccgcctga taatcaggaa cacttccgtt cgtgtagccc tcgacgaaga agcgtatttc
     1321 atcgccgctc aactcaccgc cgttgcgctt tttatgaata atatcgtaca ttcgcataaa
     1381 atcactcctg tgcaaataac agttaaatct tttctataat tataatacat ctttaataat
     1441 atatccagca taatttgaat taacaatgca aacagaccca cacagtagta aagctgcgtg
     1501 ggtccatgca tattttttat atacctaagg cttatgctca gtatcagaaa tttccgccgc
     1561 caccgccgta ggtattaccc gacgatgatt catgcgtact gctgcctgaa ccgctgttgt
     1621 tgtcgttatc ctgcggttta gcggtttttg tcacattgct gtaaaggaat atatcgttgc
     1681 tctcggttat attgaggctg ccgtttttga gatagttatt tgccgccgcc tgcatattta
     1741 ccgtatgaag cttcctcttc atgctgccga caacgataaa tgcaataaca acgcctacag
     1801 ctattgaaat gagaatccat accggtggag gcaaaacaga gccgtcatct tcgtcatcta
     1861 cagcacccat cggttcgtca tatacattgc cgttcttggc atcggttatg aactcgtcgc
     1921 aaagacgtcc gaagtattcc gccgcagcat agtaatttcc ctcaccgagg tcgcccttga
     1981 tctgattgcc tatgtactga attcctgcat cggtgaacgc ctgaatagca tatcctctcg
     2041 ttgcgatata ccagtcacgg ttctcgaagc tgacaagaag cattataccg tccttgtttt
     2101 cgccgtagcc gtaattatgt ttctcgtaaa gagcttcggc ataatccgag gttctctttc
     2161 cgtcaaggct gtttgcaaag caaattacga tatccacctt ttgcctgttt gcaatttcgt
     2221 caaactgttc aacaagctta tcttcctctt gctgcggaat tacacctgca tagtcaatca
     2281 cacggctgta ttcatcggca aatccgttga tttccgtaga atcagtaacc gcaaatgccg
     2341 gtaaaaccat acagaaacag agaacaaaag cgaaaagaat tgaaactatt tttttattca
     2401 tgactcaatc ctcctcatat aattcccaaa aggtttaaaa gtattgttat tccgtatgtt
     2461 acaatgcttg cgccgactgc cagacctgcg aaccacttga gcgccgcagc tgtatcaatc
     2521 ggaagatttc cgacgaattt acctgtctgt ccgttcatgg caaaggtgta tttctcgttt
     2581 ttccacgtcg tgttgagaat ccacacagga tacagggcat atttggcttt tccgttatgc
     2641 agatttacgg tcgaagactc cgcctcaacg gaagtgtatc cctttaccgt agaggcaaat
     2701 gtttcctcgg ttgagcgctt gattcgctcg ttggctctgt caacactttc gtccgccgta
     2761 acatcgtatt tgtcggcaag atatccggca agatacgctg tctggaaatc gacggcatcg
     2821 ccgatattga acggttcaac cgattccata aggtcatccg gcatctttga tgaaccgtcg
     2881 accggaacat tttcaaatcc gaccgtgcct gctctgaaaa cattgtaata gctcgtttcg
     2941 gtataatcgt acttactgtc gctccatgtc cttacacttg ttgcccggta acgcacgctt
     3001 gcatcggcat ctgcgtcaaa cagccagaaa ggaacataaa ttcccttaat ttcatccaga
     3061 tgattttgac ttttgaatac ctttggcaaa aggcgcttgc cggtcaaatg cttttgcaag
     3121 ccgtcttttg cggctttttt atccagctta aacggaataa tgatatcggg cttcaatgct
     3181 ccggaaaact gacccatcat aacaatggga ttgccgcaga acgggcatga ggttgcagcc
     3241 gtggtctcgt cgccgacgat ttctccgccg caagacttgc agacataggt tttcaagccg
     3301 tccgtctcgc cgctctgcca ttcgccgccg gccgaggttt cccactgcat actgtcctca
     3361 gagtcttttt tcaggctttc gtcgtagttt ttgagtgttt caacgtcaaa ttccgtgtcg
     3421 caatacggac acttcatctt ctgactttga ctgtcaaatt caattgctcc gccgcaacaa
     3481 ggacatttat attcctgcaa tacgctcaaa gctcattcct cctttatgat gcttatcact
     3541 gcttgtcgtt atcgtcaaac gggtcgccgc actcagggca gaacttcgga ggattatgag
     3601 gatcctcagg ctcccagccg cacttgtcgc agcgataaag cggtgctccc tcaggcttct
     3661 ttgcgccgca gttctgacag aacttaccct tgtttactgc gccgcacgca catgtccagc
     3721 cgttatctgc cgccggcttc ggtgaaccgc attccgggca gaatttgcct gttacgttcg
     3781 ctccgcatga gcatttccaa gcgtctgcct gtaccggctc gggcttcttt gcgccgcatt
     3841 cggggcagaa tttgcctgtt accgttgctc cgcacgagca tttccatgag tctgccgcag
     3901 gtgctgcggg tgcagatgct gcctgctgct gtgcgcccat attgtaaagg ttctgagcag
     3961 ctccgaatcc gccttgtccg cccattccga ccatgcccat tcccatgaag cctgtcattg
     4021 cgcctgccga gttgcctgct gctgtctgca ttgcatctgc cgttgcgttc gtcattctgc
     4081 ctgccatcat aaacggattg gaaccgatgc ttgccgcatc ctccatctcg tttatcttct
     4141 tcatgtcctc atctgtgagc gtaatcgggt taagtgcaat cttctcaaca gaaatacctc
     4201 tgttctctac ccactcatcc ttgagggcat tgttcattgc agctttgagc tcgctcgctt
     4261 ttgacggaat ctgtgccggt ctcagttcct gctcggcaag tgcgccgaat gcaggctgaa
     4321 gcgcatctat gaattcaacc ttgagctgat catcaatcgc atctcttgta aattcgcttt
     4381 caacattacc gcaaagtctt gtataaaact tgagcgggtc ggagattatg taagtataaa
     4441 cgccgttaca gcgaacctga acggttctgc tcatgccgat tctgttgtta actacctcga
     4501 acatgatggg gtttgccgtg ccgaacttgt tgccgattat ttcctttgtg ttgaaatagt
     4561 aaacacgctg atccttgcct gtgtcaccgc cgtaggtgaa gcgcttgccg atcgtcttaa
     4621 atgtttcttt aacgctgtca ccgaacttgc ccgagaaaat gctcggctcg gtcgaggnnn
     4681 nnnnnnngtc gaggtatcat aggtatactc gcccggctcg gcgcagacat cgacaatctt
     4741 gccctgctca acgataatca tacactgacc gtcggcaacg gctattcccg aaccgttgga
     4801 aatgatgttg tcattgccct ttgtgttgga agaacgaccg cttgtgcgct tctcaccctt
     4861 tctcataagc acttcattcg gaagtgcgtc gcagtagaaa aattctttcc actgatctgc
     4921 aagtgttcct ccgagtgcac ctgctcctgc cttaataagt cccattgtgt ttgctccttt
     4981 cagaatatat atattatttt tttataaaca atactatcaa aaacagggta attctattcc
     5041 tgttatatca gcacaccgtt acaatgatct atttcgtgct gaattatctg cgctgtgaat
     5101 ccggtatagg ttttcagtct tatttcaaat ttttctgttt gatattttat tttgattttc
     5161 ttgtaccttt tgcattttct cggtccgccg atcagagaaa gacagccttc ctccgtttca
     5221 aaaggctcgg ttttctttat gatttcggga ttgaacatcg tcacatattc ttttccgtcg
     5281 gtaaagcaaa ttatcctttt ccgctcgcct atcatgtttg ccgccattcc gacgcaggtt
     5341 tcgctgtgtg ctctcagcgt atcaagcaga tccgacgcaa tgcctgtatc ctcggcggtg
     5401 gcttccgcag attttgcggc gagccgtatc gggtcatgta taagctccct aaccatttct
     5461 tttgctcctg tattcttttt ctatccactc cataagaaga ttgactattc tccgttgttc
     5521 ggattctgtg agtgctcgac cctgcggtac tctgtaccat ttgttaagac atcctcgaca
     5581 gcaacacgca caagcatgct gtgcgacaaa gacggggtgt cccctcatcg gcgtctgctt
     5641 tccgtcattg ggaatttcgg caggcgcgag ccttttcgca acaaaatcgg cggcatgcga
     5701 acgaatcaca tcaaaccctt tttcgtcgat atacctgaca ttcttctcgt caagatgaaa
     5761 tcccgaacgg aattttgatt tctgaagctt tgccagagcc tcatcaactg tctgcattct
     5821 tattcttcct tatcgctgtt attcttctca tcacgaataa gcttttcaag tataacctgc
     5881 tttgcgatta ttttgttcat tttatcgtga agatcttcaa tcattatctc cgttttgaga
     5941 ttgaccttat aatcgttctc tgcacggcgg cgatccttct cctcctgacg gttctgactc
     6001 atcataatga gcggagcctg aattgccgca acgcaggaaa gaacaagatt aagcaagata
     6061 aacggatacg ggtcaaatgc acgtttggca agaagaatat tgcnnnnnnn nnntccaaag
     6121 tatcagcact gccgtaaacg aaaatatgaa ggcccaactg cctgcaaatt gggcaattct
     6181 gtcggcggca cgctgaccga ctgtatattt ttccttttga ggatttgcgg aaacacggct
     6241 gttcgcaagc atgttaagta tttcctcatc ggacatatca tgctttacat cggagattat
     6301 ttctctgata agctttttat ttgatttcac aaagaagact cctttgttct tttataacac
     6361 gattatagca tattattatt cattttacca gtactattga caagaacttt tatattgtga
     6421 tagaattttt acaaatattt atagaggtaa tgtaaaatga atacaaataa aattgcagtt
     6481 atcaccggtg caggttccgg aatcggtctt gcaacagctc ggatgctgca ggaaaagggt
     6541 tatacggtct ataatctcag ccgacaccct gccgaaaatg agaaaaacaa attcatcttt
     6601 gccgatgttt ccgactcttt gactataaaa gctgcaattg ataaagtcat tgccgaaagc
     6661 ggtaggattg acctgcttgt aacctcggcc ggaatgggag tttcgggagc aattgaattc
     6721 attgaggaag aagagatgaa acgtcagttt gaggtcaacc tcttcggcac gataaataca
     6781 gtcaaggctg ttttgcctca aatgaggaag caaaagcgtg gcagaattat ttgcataagc
     6841 tccgtagccg ccgtttattc aattccgttt caggcttatt attcggcatc aaaggcagcc
     6901 ataaattcct tcgtcgatgc cctgaccaat gaggtcaagt tcttcggaat agaggtaacc
     6961 tcagttatgc ccggcgacat ttcaaccgga ttcaccgccg caaggataaa gtcaaatgcg
     7021 ggtgacgacg tgtactcggg ggttatcggt tcggctgttt cggcgatgga aaaagacgag
     7081 aaaaacggta tgacaccgga ttctgtcgca agacttgttg tcaagctcgc cgaaaagaaa
     7141 cacgtcgctc ccctttacac cgcaggattg cagtataaaa cgctcgtatt tctttcgagg
     7201 cttttaccgc acaagcttgc agtgaaagtc gtcggcggaa tgtataaata aaatactgct
     7261 gcacttagcg taacaactaa atgcagcagt tgttttttta ttcggctttt ttcggcaaac
     7321 aaatgtaatg gtgaagaata ttgatatcct caggcatata aagtccgtga gaaccgcagc
     7381 ctgcatcaat ctcatggcat ccgtgatcca tcgcaaagcc caagagagtg ttgtgatttt
     7441 tccaattatt gcgaatcaac tcgcaaaaga ttgaaaacac ctgcgaattt tcggtaagct
     7501 gagcgagcgt gttcggatct tcgggcccct tgtgatgcat tattgcatca aagttttcgt
     7561 tataaacaac tatcaaatca tatttatcct caagaataag ctcacaagcc tttgcgttga
     7621 tttcaggaac ggtttcatag ataaaataat ccatgtcgcg ctcaaggaat atcttgccga
     7681 tcgagcaggt cgtatccgca ataattgccg ccttttttcc tgcacggata aatgcgtcaa
     7741 aaatcgtgtc tattgtcaga acgggctttt tatataccgt gataccgtga acctcaggct
     7801 gtgcacctgt gtacatcgtc gcaaaacaca cgggagtgac cgacggcata accgttgcaa
     7861 acggcacatg aagctccgtg cggtcaagcg tatcgtccat aaattcggga tatttttcct
     7921 gcacccattg cgctaccgca tcgggattat acataaagag cctgtcggct ttttctccgt
     7981 tgaatttttt ttcggcatag tcaacgatga cctgagccgc ctctgccgcc tctttcggcg
     8041 gttcgacacc cataaccttg cacagcgccg cacacaaggt gtcaagcgaa aggctgttaa
     8101 attcgtccat tttatacacc ccttaattga tatgggttta tgatatcata tcaaacggta
     8161 aaaagcaata aaaactgccg cagaaaactc tgcgacagca ttttttataa aattttattt
     8221 aatcactctt actctgataa cgccgtcgat tgcttcaagg gcggcagtgt ctgcctcgga
     8281 gtcaacaacc gagtaaccgt atgctcctct tactgcgctc tttgcgtctg caccgagtac
     8341 agccttaaca gcgtctgcgg cgtcagcctt gtgaataacg caggttcttg caccgtcaac
     8401 cttgtcaagc ggcattctcg gcatatttac cgagtttacg atctcgcctt tttcgatgta
     8461 agcgataagc tcatcggcag ccataaccgc acagttgtcc tcgctctcgg gagttgatgc
     8521 gccgaggtgc ggaatagcga taacgccgtc aacgccgata acctcatccg acgggaagtc
     8581 cgtaacataa gcggcagcct taccgctttc aagtgcggca atcatgtctg cgctgtttac
     8641 aagtcctccg cgggcaaagt tgagaattct tactccgtcc ttgcaaagtg caagcgtgtc
     8701 cttattgact gtatcttttg ttgcgtcatt gagcggaacg tgaattgtta tgtaatcact
     8761 ctttttgaag atgtcgttga tgtcctcaac gatctctgta tctgccgaaa gagcagcctt
     8821 aacaccctca gaaagaaacg ggtcatagcc gacaattttc attccgagtg cttctgcagc
     8881 ctgagcaacc ttgaagccta ttgcgccgag accgataaca ccgagcgtct tgcccatgat
     8941 ctccggacct gcaaactgcg acttgccttt ttcaacgagc ttgccgacat tgtcgccctc
     9001 gcccttgaga cccttgcacc agtcataagc agccgtaacc tttctcgaag cgagaagaag
     9061 tccgcaaatt acaagctcct taacggcatt tgcatttgca ccgggtgtgt tgaaaacaac
     9121 tattccctgc tctgcacact tatcaaccgg gatattgttt gtacctgcac cggcacgggc
     9181 gattgcgaga gtttcgggag cgaatgtcgt ctcgtgcatc gaagccgaac gaaccataac
     9241 ggctgtcgga ttctcacagc tgtccgagca agagtacttt ccgtcgaaac ggtcggtgcc
     9301 gcaggcggca attttgttga gagtaagaat attatacata aaattatctc cttttataaa
     9361 ataaggctgt tccgttaaag gtaaaggcaa cacaagcctt ccccttgagg ggaaggtggg
     9421 cggcggattg cgccgctcgg atgaggtggc aagtttaatc acagcaattt aatgaaatca
     9481 aaacttggct tttccacctc atcagtcgct tatgcgacag cttcccctca aggggaagcc
     9541 tgctttgtgc catctgactt taagataaca gcctgtgtgt ttttatttat gaattagctt
     9601 cctcgaactt cttcatgaac tcaacgagct tttcaacgcc ctcaatcggc attgcgttgt
     9661 agatagaagc tctcataccg ccaacggtct tatgaccctt gaggttgatg aagcctgcct
     9721 ctgttgcctc ggcaatgaac tttgcttcca tgtccttgtc gccgataacg aacggaacat
     9781 tcataagaga acggtcctcc ttaacaacag tgcccttgaa gatcttgctt gaatcaaggt
     9841 aatcgtaaag gatcttagct ttagcttcgt ttcttgcctt catagcgtca agaccgccga
     9901 tgctcttgat ccactcaaga acaagcttgc acatataaat tgtgtagcac ggcggagtgt
     9961 tgtacattga accgttatct gcgtgaatct tatagtcaag cattgtcggt gtataatcac
    10021 gcgcatgacc gagcatatcc tcgcggatga tagcgattac aagacctgcc ggagcaacgt
    10081 tcttctgagc accgccgtaa accatcgcaa acttggagat atcaagcggc tccgagaaga
    10141 agcaggatga aacgtctgca ataagaggaa tatcgcctgt gtccgggata tacttgaatg
    10201 ttgtgccgta aatcgtattg ttcatgcaat agtaaacata gtcaatatcg tcacggaagc
    10261 tctcacgggt cgtcttgggg atataagtgt aagtcttatc ctccgatgaa gctgcggcaa
    10321 caacatcgcc gtacttctta gcctctttga aagcctgatt tgcccaacgg cctgtgatga
    10381 tgtagtcagc cttgcctgtg ccgttcataa agttgagcgg aatggctgcg aactgagtgg
    10441 aagcgccgcc ctgaaggaaa agaaccttgt agttatccgg aaccttgtag atctcacgaa
    10501 caagagcctc tgcggaatca ataattccct ggaaagcctt tgaacggtga gacatctcca
    10561 taacggatgt gcctgtgccc tcatagtcca gcatctctgc tgctgccttg ttcagaaccg
    10621 actcgggaag catggacgga cctgctgaaa agttatatac tctacccata ataaaacctc
    10681 cgatagattt taggcaatgt cacataattt caactgcggt attgccttaa atattttact
    10741 gaaatattat acctttatgt taattctttg tcaactatgt tttgtttgta ttattttaaa
    10801 ttgttttatt ctttctttga acaaaatggt gaataaacga aaaaaatatg ctaattttat
    10861 gtatatacnn nnnnnnnntg gcttgcgaat tgtctgaaac tatttctgaa aggaagatca
    10921 ataatggaag ctcttaacaa aaagactgtt gaggatattg acgtaaacgg caagaaggtt
    10981 cttgtacgct gcgacttcaa cgttcctctc aaggacggcg ttattacaag cgacaagcgt
    11041 attgttgctt cgctcccgac aatcaaatat cttatcgacc ataatgcaaa ggttatcctt
    11101 tgctcacatc tcggcagacc caagggtgaa ttcaagcctg agttttctct tgctcccgtt
    11161 gccgctcgcc tttccgagct cctcggcaag gaagttaaga tggctaagga cgttgtaggc
    11221 gaaagtgcac agtcactcgc cgcttcgctt caggacggcg acgtaatgct tctcgaaaac
    11281 gttcgtttcc acaaggagga gacaaagaac gatcccgaat tctcaaagaa gcttgcttct
    11341 cttgcagata tctttgtaaa cgatgccttc ggttctgctc acagagccca cagctcaaca
    11401 acaggtgttg ccgactatct tcccgcagtc tgcggattcc tcattcagaa ggaaatcgaa
    11461 ttcattggcg gtgcccttgc taacccgaag cgtccgcttg ttgctattct cggcggtgcc
    11521 aaggtatctg acaagatcgg cgttattaca aatctcctcg acaagtgtga tacaatcatc
    11581 gtcggcggtg gtatggctta cacattcatg aaggccctcg gctacaacat cggtaattct
    11641 cttcttgagg aagacagaat cgaagatgct ttgaacatga tgaatacagc taaggaaaag
    11701 ggagttaagt tccttattcc ggttgacaat aaggtaggta aagagtacaa gcccgacaca
    11761 gaggctatga ttatcgacag tgatatcatt cccgacggct ggatgggtct tgacatcggt
    11821 cctaagacac aggctctttt cgctgacgct atcaagggca gcggaacggt aatctggaac
    11881 ggacctatgg gcgtttccga gtgggataac ttcgcagcag gtacaatcgc tgttgcaact
    11941 gccgttgcag attccggcgc tatctcaatc atcggcggcg gcgactcggc tgctgctgtt
    12001 cagaagctcg gttttgctga taagatgtct cacatctcaa caggcggcgg tgcttctctt
    12061 gaattccttg agggcaagga gcttccgggc atcgtagctc ttaacgacaa ataatcggag
    12121 gtagttaaaa tgaacaaatc acttcgtaaa gcagttatcg caggtaactg gaaaatgaat
    12181 aaaacccctg cggagactac agctctcatc aatgagatca agcctctcgt tgcagacgca
    12241 gattgtgacg tagtttgctg cgttccctat gttgaccttt ttgcagctct tgaagcagct
    12301 aagggnnnnn nnnnncgtta tcacaggcca cagcgagaga aggacatact tcggcgatac
    12361 agacgttacg gttcatgacc gcacgagagc cgctcttgac aacggcatga ctgttatcct
    12421 ctgcgtaggt gaatatcttg agcagcgtga gcagaacatc acaatggagg tcgtttcctc
    12481 tcaggttaag attgctcttg cgggcgtaag caaggaagag cttgacagaa tcatcatcgc
    12541 atatgagccg gtttgggcta tcggaacagg caagactgca acagccgatc aggctgagga
    12601 ggtctgcaag gctatccgtg agcttatcgc cgagctttat gataaggaag ctgccgacaa
    12661 gtttgttgtt cagtacggcg gatcaatgaa cgccaagaat gcagatgagc ttctcggcaa
    12721 ggaagatgtt gacggcggtc ttatcggcgg cgcatctctc aaggctcccg actttgcggc
    12781 tattgttaag gcagcaagta aataatttat atcggatata attttcgcgg ggctgacgca
    12841 ttatgcgcag accaaagaga ctcgagctct ttggtcttca taaaatgcgg cagtcctctg
    12901 attttgaaag tggtgattta ttatgaaaag accggttgta ttatgtatca tggacggctt
    12961 cggaatcaac cccggtgcaa agggaaatgc aattgaagct gcaaatactc ccaatcttac
    13021 aagacttttt aaagaaaatc cgttcactac tatcggcgct tcgggacttg atgtaggtct
    13081 cccggacggt cagatgggaa acagcgaggt cggtcataca aacatcggcg caggccgcgt
    13141 tgtttatcag atgctcgtta agatttcaaa gtccattcag gacggcgatt tcttcaagaa
    13201 tgaggccctg ctcaaggctt gcgaaaactg caagaaaaac ggcactgccc tccaccttgt
    13261 aggacttctt tccgacggcg gcgttcacag ccacattgag cacctttacg gcttgcttga
    13321 attggcaaag cgcgagggtc tcgataaggt ttatatccat tgcataatgg acggacgtga
    13381 tgtttccgta acatcgggcg tcggcttcat caaagatgtt tatgctaaaa caaaggaact
    13441 tggagtcggc gaaattgcaa cggttatggg tcgttactac gctatggacc gtgactttgc
    13501 ttgggacaga gttgagaagg catatgccgc aatggtttac ggcgaaggaa ttcaggcaga
    13561 ctgcgcagtt gacagcatga acgaatccta tgctaacgga gttacggacg aattcatcat
    13621 tccgaccgtt atcaataaga acggcatgat caaagcaaac gacagcgttg tattcttcaa
    13681 cttccgtcct gaccgtgcaa gacagatcac aagaaccttt gttgacgaga agtttgacgg
    13741 ctttgagaga aagaacggtt tcttcccgct cacatacgtt tgcatgactc agtacgacga
    13801 aacaatgccg aatgtaagcg ttgcattccc gccggaggag cttaaaatga ctatgggcga
    13861 gtaccttgct tcacagggaa aaactcagct cagaatcgcc gagacgcaaa aatatgctca
    13921 cgttacattc ttcttcaacg gaggcgagga aaagacattt aagggtgaag acagaattct
    13981 tatccactcc cccgatgttc cgactttcga tctcaagccg gagatgagtg catacgaggt
    14041 ttgcgatgca gtctgcgagc agattaaatc cgacaaatat gacgttgtta tcctcaactt
    14101 tgctaactgc gacatggtcg gtcacacagg cgtatttgag gcggctgtca aggctgttga
    14161 ggccgttgac gagtgcgtag gcagagtttg cgacgcagcg ctcagcatgg gcggcgcagt
    14221 cctcatcact gccgaccacg gcaacgcaga caagatgatt ggtgacgatg gcaagccgtt
    14281 cacaccgcat tcaaccaatc cggttccgct ctgcattgta ggctatgact gcgagctcaa
    14341 ggaaacaggt aagctctgcg accttgcacc gacaatgctc caaatcatgg atctccccca
    14401 gccgaaggaa atgacaggcg aatcaatgat caagtcggtt aaataaaata ttatataatc
    14461 aacagctctt tacctcgtgt aaggagctgt ctttcttttc atttatttgc ataaactcag
    14521 ctgtaaaaaa agacaggcct ttcgacctgt ctcttatatt gaataattta attattcgtt
    14581 gatagcggta acaacgcctg aaccaactgt acgtccgccc tcacggatag cgaaacgaag
    14641 accttcttcg atagcgatcg gagtgatgag ctcaacgttc attgtaacgt tatcgccggg
    14701 catgcacatc tctgttccct caggaagagt gataacacct gtaacgtctg ttgttctgaa
    14761 atagaactga ggacggtagt tgttgaagaa cggagtatga cggccaccct cgtccttagt
    14821 cagaacgtaa acctgacctg agaactttgt gtgaggatga attgaaccgg gcttagaaag
    14881 aacctggcca cgctcgatgt ctgttctctg gataccacga agaagagcac cgatgttgtc
    14941 gcctgcctca gcatagtcga ggagcttacg gaacatttcg atacctgtaa caacagtctt
    15001 cttcttctcg tcggaaagac caacgatttc aacttcgtca gacatcttga gctgtccacg
    15061 ctcaactcta cctgtagcaa ctgtaccacg gcctgtgatt gtgaatacgt cctcaacagg
    15121 cataaggaac ggaaggtcag ccttacggtc aggagtcggg atgtaagtat caacagcatc
    15181 catgagctcc cagatgcaag caagctcagg agcgtttaca tcgtcaccgt cgtactcaag
    15241 agccttaaga gcagaaccct tgatgatcgg tgtgtcgtcg cccgggaaat cgtactcgtt
    15301 aagagtctca cggatttcca tctcaacaag ctcgataagc tcaggatcgt caacctggtc
    15361 gatcttgttc atgaatacga tgatgtaggg aacgccaacc tgacgtgcaa gaagaaggtg
    15421 ctccttagtc tgagccatag gaccgtctgt tgcagcgata acaaggatag cgccgtccat
    15481 ctgagcagca cctgtgatca tgttcttgat atagtcagca tggcccgggc agtcaacgtg
    15541 agcatagtga cgcttgtcag tttcatactc aacatgagct gtgttaattg tgataccacg
    15601 ttctctttct tcaggagcct tatcgatatc tgcatagtct tcaaactttg catagccctt
    15661 gtttgcaaga tactttgtga tagcagcagt aagagtagtc ttaccatggt caacgtgacc
    15721 gatggtacca atgtttacat ggggcttggt acggttaaat ttttcctttg ccatttggaa
    15781 tttcctccct ttggtattaa ataattttat tcaatgcata ctgcatagtt acattgtaac
    15841 aatttccatt caaaaaagca agcctttttt gaaaataatt agccctttgt tcttgcgctg
    15901 atgatgtcat ctgcgataga cttcggaaca gccgtgtagt ggctgggttc cattacgtac
    15961 tgaccacgac cctgagtctt ggaacgaagg tctgtagcgt aaccgaacat gttggagagc
    16021 gggatattag cgtcaacctg aacggcaccc gttctctgct cctgaccgag gatcataccg
    16081 cggcgggagt taacgtcacc cataacgtcg ccgagataat cggacggaac gatgatggca
    16141 accttcataa taggctccat gagaacagga gatgcctttc tcatagcgtc cttgaatgcc
    16201 atagaaccgg caatcttaaa ggccatttct gaggagtcga cctcatgata agatccatca
    16261 taaagagtaa ccttaacgtc aacaacagga tatcctgcga gaacacctga ctgcattgca
    16321 cccttgatac cggcctcaac agcaggaatg tactccttcg gaacagcacc gccgacgata
    16381 gcgttaacga actcgaagcc cttttccgga ttcggctcaa tcttaatctt aacgtgaccg
    16441 tactgaccct taccgcctga ctgacgtgaa tacttgcaat caacgtctgc cggggttgtg
    16501 attgtttctc tgtaagcaac ctgtggctta ccgatgttag cctcaacctt gaactcacgg
    16561 agaagtctgt caacgatgat ttcaaggtga agctcaccca tacctgcgat gatcgtctga
    16621 cctgtttctt cgtcggtgta gcaacggaat gtcggatcct cttcagcaag cttctgaagt
    16681 ccgataccca tcttttcctg acctgccttg gtcttaggct cgatagcaac acggataaca
    16741 ggctccggga attccattga ttcaagaaca atcggattct tctcatcgca gagagtatca
    16801 cctgtggttg tgtacttgag gcctacggca gcggcgatat caccggcgta aacaacatca
    16861 agatcctgtc tgtggttagc gtgcatctga agaatacgtc cgagacgctc gtctgtgtcc
    16921 ttaacagagt tgtaaacggt tgtacctgcc gaaacagtac ctgaataaac tctgaagaag
    16981 cagagcttac cgacaaacgg gtctgttgca atcttaaatg caagagctgc aaacggctct
    17041 gtatcggaag aatgacgatc ttcctcttcg cctgtctcgg ggttggtgcc tctgatagcc
    17101 tcaacgtccg tcggagcagg catgtaagca atgatattat caaggagcat ctgaacgccc
    17161 ttgttacggt aagatgtacc acagcatacg ggaaccattt catttgcgat agtcgcctta
    17221 cggatgcagt tcttgatctc gtcgaccgta agctcctcgc cctcgaagaa tttctccata
    17281 agagcgtcat cctgctcggc aacagcctcg ataagctcct cgtgatactt ctgagcaagc
    17341 tctttcatat catcggggat aggctcgatt ctgatatcga gacccttatc gtcataataa
    17401 acctcagctt ccattgtaac aaggtcgatg attcctctga actcgagttc acgaccgatg
    17461 gggagctgaa tcggaacggc attacacttg agacgctcct tcatcatgtc gataacatga
    17521 tagaaatctg cgcccataat gtccattttg ttaacatata ccatacgagg tacatgatac
    17581 ttatcagcct gacgccaaac agtctcagac tgaggctcaa cgccaccctt tgcgcaaagg
    17641 acggtaacgg aaccgtcaag aacacgaaga gaacgctcaa cttcaacagt aaagtcaacg
    17701 tgacccgggg tatcaatgat gttgatacgg tgacccttcc agaaacaggt ggtagcagca
    17761 gaagtgattg tgataccacg ctcctgctcc tgagccatcc agtccattgt tgctgcaccg
    17821 tcatgtgttt cacccatctt gtggttaaca ccggtataga aaaggatacg ctcggtcgta
    17881 gttgttttac cggcatcgat atgagccatg ataccgatgt ttcttgttaa ttcaagtggt
    17941 acttgtcttc ccatataaac
//
LOCUS       DAWBSW010000019        17634 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_709_length_17634_cov_2.982877, whole genome shotgun sequence.
ACCESSION   DAWBSW010000019 DAWBSW010000000
VERSION     DAWBSW010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 17634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17634
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_17634_cov_2.982877"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3094..3103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4455..4464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16807..16816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5027 a         3428 c         4131 g         5018 t
ORIGIN      
        1 ttgctataca gctaagtctg attatatatt attttacttg aaaaaaacgt cggttcatag
       61 taaaataaat ttattatatg cttacttgag tcgggagaaa aatatgagca aagaagaatc
      121 taagatgtta cagggaatat ctgtacttgc tatggtctgt ttgcaccttt ttgataatct
      181 taattattct gatttattta cgcccttgat tatgctcaag ggataccctt tgatttttta
      241 ttttgcacag ctttcggatt tctgcgttat gggatttgcc ttttgcagcg gctatgcaca
      301 catgaaattg tttcataaaa agaattatta caaaagaaga ctgataagct tattgtctct
      361 ttactgcaat ttttggataa ttcttctttt gtttaccttg atcagcgtcg ccacaggtca
      421 ggctgatttt atgccgggag attttgtgaa gtttctcaaa acttttacaa cggtttcggt
      481 ttcttataac ggcgcatggt ggtatatgtc ggtttatgcc gtgattgttt ttatctctcc
      541 gattgtgctc aaagcgactg aaaaaatgaa ctcaattgca gttttgctga tatcgtttgc
      601 aatctattgc ggcggttatc ttatgcggtt taaatacgga accccgtatg aagcgataaa
      661 ttggttcggt ccgttcggaa caactttttt tgaatacatg atcggtgttg tttgctgtaa
      721 ggagcggttt ttcgaaaagc ttgagagggc tgcaacaaaa gtaaaaccga tatggagatg
      781 tgtaatttcg gcggcggtct ttgttgcaat gcttttgggg catacacttg ttatcagatc
      841 gttggttata gctccgctga caggcttttt gattatatgt atttttaaat tttgcccgag
      901 attgcggccg gttgacaaag ttttcggctt tttcggaaca cattcaacaa acatttggtt
      961 tacgcatatg tttttctatt tggtgctgtt tccaaagctt gtatacagag cggaataccc
     1021 tatcccgata tttttattta tgcttgcaat atgcgtcgcg gtgtcgtatg taataaatct
     1081 tatttattat cccataaata agcaggtcag aaaacttgaa aatttacttt aattatatta
     1141 taaagaggaa taacccatgc tttcacaaaa aatcgaaaaa attttgcaag aggttcagaa
     1201 gccgggacgc tacgtcggcg gcgagttgaa cagcgtaata aaagataaga acaaagtaga
     1261 cgtcagatat gcattctgct tcccggatac atatgaaatt ggcatgtctc atctcggcat
     1321 gaagattttg tactccgttg tcaacaggct tgactatgcg tggtgcgagc gtgtgtttgc
     1381 tcccgatgtt gatatggaag aaaaaatgcg tgagaacggt ataaagctgt gggcgcttga
     1441 gagcggagat ccgcttgatg aatttgatat gctcggcttt acgctccaat atgaattgag
     1501 ctattccaat attctcaata tgcttgacct tgcgggaata cctctctatt ccaaggacag
     1561 aaccgagctt gctccgttgg ttgttgcagg cggtccgtgt gcgtgcaacg gcgaaccgat
     1621 tgccgatttt attgatatct ttatgctcgg cgacggtgag gaaactacgg ttcagctcgt
     1681 tgaccttatt cgcaagcaca aaaaactcgg ctcgccaaag atcgagcttc tgcgtgaggt
     1741 agcacagctc aagggctttt atgttccttc gttttatgat attgaataca acgcggacgg
     1801 tacaataaaa agcgttacaa ataaggaaac cgcacccgaa aaggctgtca aggcggtagt
     1861 cgaggatatg gacaaggtgt attatccgga gagctttgtt gttccgtcaa tcgaggttgt
     1921 ccatgacaga gtgacggcgg aaattttcag aggatgcata agaggatgcc gtttttgtca
     1981 ggcgggattt ttgtacagac cgataaggga gaagtctcct gaggtcgttg aggagcaatg
     2041 tagaacgctt tgcgaaagca cgggttatga cgagatatcg ctttgctcgt tgagtacaag
     2101 cgactacaca gggcttcaac cgttacttac gtctatgctg aaatggacaa cggaggaaaa
     2161 tataaatgtt gcgcttccgt cgctgagagt tgataatttt tcggacgagc ttatggaaaa
     2221 gctgaccgag gttcgccgaa gcagcctgac ctttgctccc gaggcaggaa ctcagcgctt
     2281 gcgtgatgca atcaacaaaa atgttacata tgccgatgtt atgaaaaccg tgaatatggc
     2341 tttttcaggc ggttggactg ccgttaaact ctattttatg atcggattac cgaccgaaac
     2401 tcttgatgat gttgcaggaa ttgcacagct cgggcagcag gtcgttgatg aattttaccg
     2461 caatcccgac agacctaagg gcaaaggcgt tcaggtaagc ataagtgcgt cgtcattcgt
     2521 gccgaagccg ttcaccccgt ttcagtggga gccgcaggac accatggagc agcttgaaga
     2581 aaagcagaag catttgtatg caagcgttac gacgaagaaa atccgaattt caacccacct
     2641 tactccgacg agcttccttg agggcgtgtt tgcaagaggt gaccgccgtc tttcaaaggt
     2701 tttggagctt gcatggaaaa aaggatgtaa atttgacagt tgggacgatc attttgactt
     2761 tgaaaaatgg atggaagctt ttgatgaggc agggcttgac ccaagcttct atgcaaatcg
     2821 ccgccgtgat tttgacgaga ttctcccatg ggatcatctt gattacggca taagcaaaaa
     2881 attccttatg aacgagaata aaaaggcgca tgaaagcgtc acaactccgc attgcaggat
     2941 caaatgtgca ggctgcggag caaaccgact caacggaggt aagtgcgatg cgcgcagtga
     3001 ggcttaattt ttcaaaaaca ggcagggcaa tttatatttc ccaccttgat ataaaccgaa
     3061 tgatgacccg tgccgtcaga cgggcaaagc tgcnnnnnnn nnnaaagctg ccgatgtggt
     3121 acaccgaagg attcaatcct cacccttatt tgacctttgc tcttcctctt tctctcggtc
     3181 aatcgagcga ctgcgaatac atggatatcc gcattgaggg cgatattaca gacgaggaga
     3241 taatgaacag gcttaacgcc gttcttcccg agggtgtgaa aattctttcg gtttccgctc
     3301 cggtttacga tgcgaaggag attgaaaagg cgctttattt tgtgaagctt gttttcaagg
     3361 atgccgagac atctaagatc tttgccgaaa gggcgcagac ccttgccgag ggcgaagaat
     3421 tgcttgccga gaagagaggc aaaaagggtc acagaaaggt tatgaagcag ataaatctta
     3481 ttgattttat ctatgatatg aaattctcaa cggcggataa tattgtgaat ttgcaatgtg
     3541 ttctcgccgc aggaaatacg aacaatctta atccgaccct gcttgtcgaa acactcgaaa
     3601 aagagatagg cattgagcat gagatggaat atattgtccg caggaagctg attaccaagg
     3661 acaataaaac tttcaaataa aaggagacag ccgaaatggt tcagccgata gaaagaatta
     3721 aaagagaaaa gattttttcc gttgttttga cggttcttct tctcggcttt gtcggcatgg
     3781 tcttttatat aaatttcagc atcaatcctg agtattacga tggggatatt tataatgata
     3841 tcaattatgc aaaagaagcg tggaaagcga agtctctttt tccgaaggat tggattttcg
     3901 gcaatcagac ctatgttgtg gcaactcccg ttttggcggc gttgttttac ggaataacag
     3961 gtaacggttt taccgccatg gcaattgcgt catgcattat gactgtgttg acactgctga
     4021 cctatgactg gatgacaagg acgctgtttt cttataacga acgcacggca ggctttttat
     4081 ttatgatcgg atttctgctg ctcaaagccc atgtggcaac aagtcagcag ggaatacagg
     4141 cttttttcac tatggcaagc tattatgcgt gctatcttat aaatgcgttt atcgtttacg
     4201 gctgttatgt aagaattcgc cagggaaaat ttacgggaaa gcatattatt ccggcaatta
     4261 tcggtgttgc gctcagcttc ggaacgggta tgcaaagctt gagacaaacg gcagttatgg
     4321 cattgccgct cgttgcctgt gaggtgctgt tgataataat ttattcagcc aaggataaaa
     4381 aatttgcaat atcctattca acgctttttt cggcaatcgt gtttattgca aacattgccg
     4441 gacttgtcgc aatgnnnnnn nnnnaatgag gtttatagaa ataaatcaaa attctatcta
     4501 cgggacaacg gcgtttgtaa gcagctttaa ggacttcttc agaaagcttt ttctgaatat
     4561 ggagagcgtg gcattgacat ttggcctcga cgcactcgaa ttaagggtgc ggctggttgc
     4621 ttctataccg tttttgctga ttattcttat cggatttata ctttgcgtta aggattatat
     4681 caaaaacaaa tgcaacgaac agggaagctt tgttttagta atgcttctta ctctcggctg
     4741 tgtatccgtt tttgccgcag gtgttctcac cgatgttgtt aaccgtgcgc tttattattt
     4801 catgatctat cctcttctgg cggtctgcgt ttcgtatatc atcgtaaaat ttgagaaaaa
     4861 aagagacgtc cttttctctg ttgttgctgt ttttacggca ggaatgataa tattccgcac
     4921 tgtcggcgca gtagaagaga taaaggcggg caaggagaaa aattcgacgg cgcatcagat
     4981 tgcaaattat atgctcgata acggctatga tacaatctat tcggtgttcg gactcagcgg
     5041 cgttatggac ggagcggaaa atgtagttgt tgcctcggga gataaaattc atattgttca
     5101 attcaaaaac gttgacagct caaagcccat gaagccggtt gaataccttt gcgttaagga
     5161 tggctacaag cagtgggata attcaaagag cttatatctt ctgagagacc acgaattgcc
     5221 gaaggtcaaa aaaattgccg aaaaatatga cgttacgatg accgaaaagg ctcggttcgg
     5281 tgacggactg tatctttaca gcatgtcgga gaatatttgt atctataccg atatgcaaaa
     5341 atagtggtga aaaacatagc gtataacaag atgttgtgaa caaatatatt tcagaaaaaa
     5401 attattgatt ttttcctata aatcatcaaa aaatacttga aataaatcga tcaatatgtt
     5461 aatatattta agcatttgtg accaccaagg cggcagtgtc ggtttggcgg ggtttaatgc
     5521 ccaacattaa agcggacggt cctctgtcgg tttaaccgtt aagaacgcct gaaattctat
     5581 gggaggtgtc tgtaatggac gcacttaagt taatcgctca ggacagcctc aaggctgacg
     5641 tttctgaggc aatcaaggta ggtaacacta tcaaaatctg ggttaagatc cgtgaaggcg
     5701 agagagaaag actccaggct ttcgagggta ccgtaattgc cattaagggc agcggagttt
     5761 cccagacatt taccgttcgt cgagtttctt acggcgttgg cgttgagagg gttttccctg
     5821 ttcattcacc caatgttgac aagattgagc ttatcagaac aggtaaggta cgcagaagca
     5881 agctctacta tctgcgtgac agagtcggta aggctgctaa ggttaagcag agcatcggct
     5941 aatgtaatct ttgattacga aaacgagctg aggggtatgg cggtggttcc atgccctttt
     6001 gtttttatta agaattatat aattagcggt tttgaaccga ttcgatataa ggaggtaatt
     6061 caatgttatt aagcagacgc agtgttcagg aaagctggaa tgaatacctc aaaaacggca
     6121 tgactcccga gcaggctaaa aaggaacgtt cacgttgggt aaaaatttac gatcttgtcg
     6181 gctcggttat aacggctttg gttctggttt ttgtcatatt tactttcatt tgccgcccga
     6241 cgagcgtggt cggcacctct atggttccga cgcttcagga cggagactgg ctgctgacaa
     6301 tggctaagag tgagtataat tacggggata tcgttattat aactcagccg aatgtttttg
     6361 atgagccgat tgttaaaaga attatagcaa ctgagggaca aacggtagat attaactttg
     6421 tttccggtca ggtttttatc gacgggcagg aaatcaagga gtcatatatc aatgaactga
     6481 ccaagcgttc gggcgatgtt acgttccctc ttgttgttcc caagggtaag gtttttgtta
     6541 tgggagacaa cagaatgcac tcgacggaca gccgaagcac cgatatcgga tttattgata
     6601 cgcgatatat tctcggtaag gcaactgtga gagttctccc gtttggagat atgaatatat
     6661 atgcgaattt tcaacagggt gaggaataat ccatgaatga aaataacaca aacgaaacgg
     6721 cggcagaggt tgccgaagaa agcaaaccga agggttcatt tctttatgag accgtatcgg
     6781 tttttgtaac tgcgcttgtg attatagcga ttatgttcac gtttcttttt cgctttgtcg
     6841 gagttgacgg atcgtcaatg gttccgacgc ttgaaaataa cgactggctc atcgtttcgg
     6901 caatcaatac ggatattgag cagggcgata tagttataag tacgcagccg aatgcttttg
     6961 acgagcctat tgttaaaaga gttattgcca agggcggaca gacggtagac attgattttt
     7021 caacaggtaa ggtctatgtt gacggcacgg agcttgacga gccgtatgtt gccgcgccga
     7081 catatgctca ggagggcaac acttttccca tggttgttcc cgagggcaag cttttcctga
     7141 tgggtgacaa cagaaacgat tctactgaca gccgttcgcc gatggtcggt tgtgttgacg
     7201 agcgctatat tctcggcgtt gtcaagtgca gacttatgcc gaacttcaca tttttcggca
     7261 agtcggataa ttaagataat atcgtataat aaaaatcacg gtcgaatcct ttggttcgac
     7321 cttttttcaa agcagggcta ttttataaag cctttgattg accgtgaggt atatcaaagg
     7381 ctaaaagagg ggtataaaag tgaaagaaag aaaaatgtat ggtatcagaa ttatcgctgc
     7441 ctttatgtca tttgttactg taatttcatt tgcgggctgt tcggctaaga aaagcgaggg
     7501 cagtaagggc ctgactactg agtcgaatat aacaggagat aatgaaaaaa acggtataac
     7561 ggtaacagag cctgacggca ataagccgac tgcaacaggg gctgtaaaca gtaaagacgg
     7621 aaaatcgtca tctgataatt caagcaagac aaataatgca aacgctacta aatcattaga
     7681 cagcatatta aaagagcttg aaaaaaacac tgcaaaattt tctcccgatg tcaggagtta
     7741 tttaaaagac ttgataaaga atgcttataa taactatgat aatttggctg acgtattatg
     7801 caaaagttct ttgcccgata aatgtacatt cttagaagaa aaaataatcg atcccctaaa
     7861 aagagttgat actattgata taatttcaag caacaatccg aacttttatg agttgaaaag
     7921 cgagtacgaa acatcaagat ggattgaaga aaaaaagaaa attatagtat tttgtgatgg
     7981 ctttaatgta gatgagtatc ctcaaatttt aatggaagaa ataatacatt cgggtcagga
     8041 aaaattgaat acagctaaat tgggatattc cgagtattgt atttttgcag aaggcgaagc
     8101 caatgcatac gcatggcctc ttactttcgg caagataaat aattcaggaa ttgatatatt
     8161 ttatgatgat gaatcgatga acaatgccaa ttacggttac ggtaccggtt atttttattg
     8221 tgccttagcg actaaatatt atatgtatct tttaactctt acagattatg acacagtaaa
     8281 taaatttaaa aaaacattag attccgatgt aattacagat gaattaagca aactttatgg
     8341 tataaacgga gccgaatttt acacgaaaat gaaagaagta atagtagatt cggcttccag
     8401 tatatatgac aaaagaactc ctcaaatggt atattgtgaa aaagtatata atacttgcat
     8461 gagtaaaaaa atcagtgata tcggcaatgc caaagaagct aaggaagtgc ttaatttata
     8521 tagatatatg aatattcaat atggctatga gtattctttc tatgatgaaa aaaatgatac
     8581 atatgttgat aagactaatg aaaaaacagg caatttgaga acaattgata atctatataa
     8641 taagtgcaaa aaatacggtg tttttagtgt ttcggataat agcgctaaag ataaaaaagc
     8701 tttttatgca tttgtttctc cggaacgtaa ttctgatgaa aacatttatt ttatatcatg
     8761 cagcaacgca gatattaagt tgtccgggaa taatctgacg gttatcaata atctcggaac
     8821 ataccaaaca aatatcgatg atttaagttc aaaaacggtt aaatcatatt ataattatct
     8881 tgataatttt cctaacttaa ataatgttaa atcttaaact aaaacggaga ataacagcga
     8941 acggaagtga ttaaagtgga tatgcatcaa aaaagaaaaa tcagagccga gaagaaaagc
     9001 aatgaggatc aaaagaactt ttcaaaagta tccgttcagt ggtttccggg acatatggca
     9061 aaaacgagga ggcttatcaa ggaaagtctc tcaagagtgg actgcgtgac cgagatactt
     9121 gacgcccgta ttcctctttc cagcagaaat cccgaaatcg gagagataac agcaggcaaa
     9181 ccgaggataa ttcttctcaa caaatgcgac atggcagacg aaaaggtgac cgcacagtgg
     9241 ataaaatatt ttcgctctac gggagcggag gcaatggcgg ttgactgccg ctcgggcagg
     9301 gggctcaacg gctacacgca gcttgtacgc aaggttcttg ccgataaaat tcagcagaac
     9361 aacgaacgcg gtatggtcgg aaaagctctg agagtcatgg ttgtcggaat tccgaatacg
     9421 ggaaaatctt cttttataaa taagatggca ggcagagtcc gtgctaaggt tgccgacaga
     9481 cccggagtta caacgagcaa tcaatggttc ttcatcggca acggaattga acttcttgac
     9541 actcccggtg tgctttggcc aaagtttgat gatccggttg tcggcgaccg cttagccttt
     9601 atcggctcgg tcaaggatgc tgttctcgat gtcgaaacaa tggcggtcag actgatcgac
     9661 gttctccgtg agggctatcc cgagcggctt gccgaaagat ataagctcga tgatattacg
     9721 gatatgcagt cgtgggaaat cctcgaagca atcggcagga aaagaggtat gctcataagc
     9781 ggtggcgagg taaacaccga acgcgcctcg gttatgctcc ttgacgagta cagaggggga
     9841 aaacttggaa agataacaat ggagtttcct cctgcttgac ggatcttttt ccgtgatacg
     9901 aattttattc ctctcggcat atatgtatat tgccgtcaag aaataaaatc cgcctaacga
     9961 aaaaatctca cgtcaagaat aaagacgatt ctttatgatt acgcatccga tctctataat
    10021 ctaacatcta aaatctaatt tggggatgac cgcaatgaaa acggaagtaa aaattgattg
    10081 gttcaaatat gaaaacgaag ccaaagcgga cggttataac atagtctgcg gaattgacga
    10141 agccggcaga ggcccgcttg caggaccggt ttgtgcggca gcggttattc ttcccgacgg
    10201 ctgtataatc gagggagtca acgattccaa aaagctcacc gaaaaaaagc gtgagcagct
    10261 ttttgacgtg ataaaggata ctgccgttgc ctattctatt gcgaccgcag acgaaaagga
    10321 aattgatgag ataaatattc ttcaggccac atatcttgcg atgaaccgtg cctttcgtgg
    10381 attgagcgta agaccggata tggcgctcgt tgacgggaac cgtgatcccg gtctcggtat
    10441 tccgacaaga acaattgtta agggcgacgc aaattcaatg tcgattgccg cagcttcgat
    10501 tcttgcaaag gtgacaaggg atcgctttat gcttgaaatg gataaaaaat atccggaata
    10561 tcaatttgcg aagcacaagg gttacggaac aaagcttcat tatgaaatgt tggataaata
    10621 cggcgcttcg gaaattcaca ggatgtcgtt tttgaggaaa tactatgaaa ataaataaat
    10681 cgggtgtttg gggcgaaatt tttgccgcaa gatatctcag agacaacggc tacagaatta
    10741 ttacggctaa ctttcgatcc cgcttgggcg agatcgatat cattgcacaa aaagggaatt
    10801 atatgtgctt tgttgaggtc aagacgagag gcgaggaaag gctgtatgag ccgaaagagg
    10861 cggttgattt ttcaaagcag gaaaagctga tatcaacctc gaagcttttt atgaaagcct
    10921 atccgaacgg gaagcagccg cgatttgatg tctgcgaggt ttttcttgac ggtgggttta
    10981 atctcataaa gataaattac attgaaaatg cgtttacgga aggcatttaa ttgctgtttt
    11041 ttataatatt tcattgaatt gtttttttac ttatgataca attagttcag aacttaccaa
    11101 aggagaggta atatgtttta cacaagcaca agagataaga gcataaaggt aacttcctca
    11161 caggcgattt ctcacggcat ttctgccgag ggaggtttgt ttgtaccgga ggagatccct
    11221 catataacaa tggacgatat cgtgcgtctt gccaactgcg attatataga aagagcaaag
    11281 gaaatacttt cactttatct tacggacttt acccccgagg agattgattc ctgcgttaac
    11341 ggcgcctacg gagagggcaa attttcaagc gataaaattg cgccgcttca tacattgaca
    11401 aaaggcgttg aaatccttga gctgtggaag ggcccgacct gtgcattcaa ggatatggct
    11461 cttcagcttc ttccgcatct tcttaccgtt gcctcggcaa agagtgagcc gggcaagcag
    11521 atagttattc ttgtcgcaac ctccggcgat acgggcaagg cagcgcttga aggcttcaag
    11581 gacgtcaaga acacaaagat tcttgtattc taccccaatg acggcgtttc tccgatgcag
    11641 aagcttcaga tgacgaccca ggagggctca aatgtcggcg tttgcgctat cgaaggtaat
    11701 tttgatgacg cacagacggg agttaaaaag atttttacgg acaagtcggt tgccgcaaag
    11761 cttgccgagc agggcatgat gttctcgtcg gcaaactcaa tcaactgggg acgtcttgtt
    11821 ccgcagatcg tttactatgt atccgcctat tgcgagatgc taaacaaggg cgatatcaaa
    11881 gcgggtgacg agatcaatgt tgttgtacct acgggcaact tcggtaatat tcttgccgct
    11941 tactatgcaa aggagatggg cgtgcctatc aaaaagcttg tctgcgcctc caatgcgaac
    12001 aatgttttga cagacttcct caggacggga acctataacc gtgtaagaga tttctacacg
    12061 accgtttcgc cgtcaatgga tattcttatt tcgtcgaatc ttgaaagact tcttttcctt
    12121 ctctcgggca tggatgatgc cattgtcaga gagtggatgg gcgagctatc ttcaaaaggc
    12181 tcatacaagg taagtgacga tgtttttgca aagctttctg agctgttcga tgcaggctgc
    12241 tgtgacgata acggcaccaa ggcttgcatt gccaaggaat ttaatgagca caaatatctt
    12301 tgcgataccc atacggcagt tgcagtctcc gtttatgagg actatgctaa gagaacggga
    12361 gacacaaccc cgactgttat agcttcaacg gctaacccgt ttaaattcag caaggcagtt
    12421 cttgacgctg ttcgtcccgg catgaccgaa aatcttgatg agttcgagat ggttacggct
    12481 ctgcatgacg agacgggaga ggtatgtccg ccgcagcttg caaaccttaa gggcaaaacc
    12541 ccgagattta ccggctgctg caagaaagag gatatggagc aggtcgtatt tgatttgctg
    12601 aaataattct atattattag agatacgccg gatgaaaaag gcaggattta gttattaaac
    12661 agctttgtgc tgtttaataa ctaaatattg agagggtgtt tcttatgaaa aagcatgttc
    12721 tcagcttggt ttcatttatt gttatttttt ctgttgtgtt ctgcaacatc tttgtgacca
    12781 atgcttcaag tgttgtcggc gacattaacg gcgacggcag ggcgaactcg tcggatgcat
    12841 tgatcgttct tcgatattcc gtaggacttt ccacgggtat caaggatatg tctcccggtg
    12901 acttaaatca tgacggaaag ataaacactg ccgatgcact tattatactc agaatatgcg
    12961 tcggtctgga tgaccccaag aactatacaa caacggcgaa gccgacaacg acaaagccgg
    13021 ttcagtttac cccgtctgcc gtcaaggagg tttctccgtc atctgtaact cattatgcag
    13081 gaacaattca gcgcgcatgg cggatttcat ccaaagtttc aagcgttgag atccataagg
    13141 ttgcatacgg tcctaaaatc acgcagaact ttttaaagag tcaggacagt gcattggatg
    13201 tttcatctaa gagcgttaca tatcagccga tttgcaatgt tgccattgtt tcatgtgctc
    13261 cgacaacggt taaatatgct attacatcgg tggttctcgg aaccacaaag agcaagcagg
    13321 aggatatctc caaaaaggtt ggtgcattgg tggctatcaa cggcgaatca accagcgcag
    13381 ccacggctat ggcgataaaa aacggtaacg catatttcca gaacggtgcg ggcagagagg
    13441 ttatgcgttt gtacagaaac ggcaagtgcg attacggccc gcttaataag cagaatcaga
    13501 atcagcttat caaagacggt gtgtttaata cggtcagata tcattatgtt cttatcaagg
    13561 acggtaagaa gatcaataac aatgagtctt attatcacaa ccgtacggtt cttgctcaga
    13621 taagcgcaaa caaatacatc cttgcggttg ccgaatttat gccgttgaat acgattgccg
    13681 atcttctttt gaaatacggt gcaaaaaatg ctgttgtaac caatggcggc aattgctcat
    13741 atatgtatgt cagaggaatc ggaaacgtta ccggaacaag agcaactcag ctcaagaatt
    13801 tgaacaaggt aaatgttgtt gaaaccgaat tctttgcaaa tcacggtatg ctcggtctta
    13861 acagtgccgg aaagccaaag ctcggcgcac cgtgcagcga tgaggtcaat tctgtgtttg
    13921 ttatgtaaca ttcgcttgat tttcaatgca gtttaattga agaaaactgt cacattaagc
    13981 ctgtgatata cagccttgat gtgacagtcc cttttaaaga agttttactt gcttacgtcg
    14041 ctgcttggct caaaggttgc gcagattgtt tccgcgcagg tgcaagcgct tgacgggccg
    14101 acttcgatct tgccggctac gcacttgcaa ccctcttcgt tatagatgca gttgctagca
    14161 tggcacttga tatctttaat ttcgtgcttc attgcaaatt accccttttt cagatttgtg
    14221 aaacgtttca cgcttatatt atctgcttaa tctcgttttt tattcttttt ttgaggaggt
    14281 gaagaaatgt cattatttgc cattgcagat acgcatcttt cttttggtac ggataagccg
    14341 atgaatgttt tcagcggttg gcaggactat gaaaaaagac ttgaaaaaaa ctggaacgct
    14401 gttgtaacgg aaagagatac cgttgtaatc gccggtgata tttcgtgggg aatgaagctt
    14461 gaagacacat tggaggattt ccgttttttg aataagctga acggaacgaa gattattata
    14521 aaaggcaacc acgattattg gtggtcaact aagagcaaga ccgaaaaatt tttgaaagaa
    14581 aatggaatcg aaagcataaa aattcttttt aataatgcgt ataaagttga caatgttgca
    14641 gtttgcggca caagaggctg gttcttcgaa tgctcaaagg atgaggatca aaaggttctt
    14701 gcgcgtgaag tcggcaggct gaaaacgagc attgccgaag caaaaaagct cggcggcgaa
    14761 gttatcgcat ttcttcatta tccgccgata agtttgaccg agacctgcca tgagatttat
    14821 gatacattgg ttgaggagca gatcaggaac tgctattacg gacaccttca cggtaattcc
    14881 gtaaactggg cattcagagg ggaaagcgac ggagtgaatt ttgatcttat ctccgccgat
    14941 tacttaagct tttgcccgaa attgataaga aaattttaat tacagacaga aaaaagctcc
    15001 cggaaaaaat ccgagagctt gattttttgt tgaaacaaac ttacttgagt tcgatctctg
    15061 cgccgacttc ctcgagctta gccttgatgt cctcagcctc gtccttagaa gcaccttcct
    15121 taacagcctt cggtgcgccg tcaacaagag cctttgcctc agcaagtcca agacctgtga
    15181 tgtccttaac tgccttaaca accttgatct tctgtgcgcc tgcgctcttg aggataacgt
    15241 caaactctgt cttctcctca actgccgggc ctgctgctgc cggacctgct acaactgctg
    15301 ctgcagctga tacgccgaac tcatcctcta ctgcgtgtac gagctctgaa agctcaagaa
    15361 ctgaaagagt cttcaactct tcaacgatag cattaatttt ctctgatgcc atggtaattt
    15421 acctccgtaa taatttagta attttttaat tttagtttcg ctgacaatta agcctcagcg
    15481 ggagcctctg ccttatcggc gatagcctga acagcacgag caaagcctgc gataggtgcg
    15541 ctgaatacat ttgcaaccat agagagcaat gtttctctcg agggaaggtt tgcaagatcc
    15601 ttgagcttct ccatcgtcat tacctcaccg tcgaggtaac ctgctttaag cttgaagttc
    15661 tcgtgatcct ctgcatactt gttgaggata cgggctgcgg ctacatagtc gtccttggaa
    15721 gttgcgagag ctgttgttcc gtcaagaacc tcacccatgc tttcgaggga cgtaccgttg
    15781 attgcgagct tgaggagagt gttcttaaca actgtgtact caactcctgc ttcacgaagc
    15841 tccttacgaa gagctgtatc gtcggcaacg gaaattccct tgtaatctac gattacacct
    15901 gcgcatgaat tgctgattct gtctgctacg gaagcaacta tctgcttctt ctgttccaat
    15961 acgcttgcac ttggcatttt tttcacctct catgcaatga aataggtgcg tgtaaacgaa
    16021 aataaatccc tgtccactca ccgtgaacag ggatatgaca tacaaatatt gatccgaata
    16081 gctcggttat cgtatgctcc cattctcggc aggcggtctg ttaaaaccat tacgctgttt
    16141 tgcacctgct gtctttgaaa agaacagctt agttatatta gcacacgttt ggatttttgt
    16201 caatacttat ttttgatttt aagcaaatta tttgccgaaa acgggataaa aggagataat
    16261 aatcttgttg agaaggtcta taacaagctt aaagagtttt tttgccataa caataaactg
    16321 attttcattg gcttccttga ctgcgtagcc gggaatagca ccgtcacggt tttccactcc
    16381 gtcaacatca ctgaagtatc cgccccaaag gccgtaggca attgcaaggg cctccttttc
    16441 ctcgggagtg gcaaaaccgt atttttcgct gaaatcgcgg agcatattat cggcaacgct
    16501 gcccttatag ccgagcttgc caagcatctc gtttgcaccg gaaagaatgt cgctgtctgc
    16561 cgttgcggcg attgatttac gaacggtgaa cgcaagcagc cttatgagaa ccgtaaaatc
    16621 attcgagttg agattatatc tcttgtcgcc gattttgcat ctgagcatgg ccagcgtcat
    16681 ggcatcattg agagttttat actcactgtc gggaatttca aggccgtatg ccgctgcttg
    16741 ctcttgaatt cctcccttgc cgtaaatcgg tgtatcggac attttcttta ttgtatccgt
    16801 aacaggnnnn nnnnnnaaca ggtttgagct tggcataaag tgcgcttgac tcttcgacgc
    16861 cgagcgtttc gggggagata ttttctctca ggtcctcgga atactcccta taagacatgc
    16921 tccaagtgta tgtttgaaga ttattcttca tcatttcgag agcttcattt gagaagcctt
    16981 tgcaaacttc ggcaagcttg tctgcgtcga tatggttgat tttctttgtt tcatagctaa
    17041 ttttactgtc ggttacgtta aaatatttgt attcggcggg gaagttgccg aggcttgaca
    17101 ttgagaaatc ataaattgtg ttgccaagaa agcttgtgta tgattttaca ttgttgcagt
    17161 gtgtatgacc cgaaaaaaca agcttaacac ccaagtcggc aagaagtccg gcaaaggaat
    17221 atggggtatt aacaatatag tttttttcgt tcagcttctg aaacgggttg tgctcaagaa
    17281 ggttgtggtg catgatcatt ataacctttt tgccttgctt ctttgcggct ttgatttgct
    17341 ttaccgccca gtccattctt gtaatgtcgt tatttggaac aacccgatat ctttcatcac
    17401 aggtatcaag cgcaacaaga gtatattcat cgttgaggtt tgcgctgtag gagcaggttc
    17461 cctcatctgt tgaaagagcc tcgtcgtagc cgaattcgtg ataaatttcg gtgaattttt
    17521 tatggtcaac gggcatatcc tttgcgttgt catggttgcc gttgatgaca tagatcttca
    17581 gccccggaat atcctgctgc agctgctgca gctgctttgc cagcgccgcg tggc
//
LOCUS       DAWBSW010000020        16513 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_772_length_16513_cov_2.913902, whole genome shotgun sequence.
ACCESSION   DAWBSW010000020 DAWBSW010000000
VERSION     DAWBSW010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 16513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16513
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_772_length_16513_cov_2.913902"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1077..1086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2134..2143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4251 a         3847 c         3344 g         5051 t
ORIGIN      
        1 gccatatact gagtaagaag cgtcttgaat tccgaaaaat cgtctgtcaa atatccctta
       61 gtataaacag ggacaagttc ttttgccgcc ttcttaatga attcctccga aaaagcagga
      121 atttcatggc tgtttggatc gtccttgtca aggtaaatcg gtgaacggcc tcgcacatag
      181 attatcggca cctcatcaca gtcacaggtg tctttcgcta ttgcacttat gggcgcaata
      241 accataacga agattaccaa actcagaaac actgctaaaa attttttcaa aaccatctct
      301 ccttttccag gttaagcttt atttcggcta ttccgcggga gttaattatt tgaacagctt
      361 acccaaagtc tcaataatga tcttcaatac atttactata agctttttga taagagtaaa
      421 gaatccgggt ctttccagcg gatttccctt gtcctcgcgg gtcatcggtc ttattgtatc
      481 gccgttattt tcctctccct catagataat gtactgcggg tagttttcgt cactgaacac
      541 cgtcatcggc tctccgtcgt gcctgaggag ctgataaata aacgggcaaa gtattctcgg
      601 ataacagtta tgcttcatgt tttgtatata ccacgttgaa tccgggaaaa gcgttgttga
      661 tgcatcaacg gcaagatcgg gagaaatata tttatccgtt ccgttttgct ttgcctgagc
      721 cacgtagtca tcgctgagct ttttgccgat cggtgccgtt gttgtaccgg gtgcctgctg
      781 ctcgcaggta acaatcatgt cgctctgtct gtcggcattg taaaccacag gataaagctg
      841 ataaccgtac tttgcgatta tagaaacgtt cactccgtcg gccttgatat ctttatataa
      901 cgttgtaagc tttgaaccga ctttttcgtg gtaatcattg atttttttaa tcaatcctgc
      961 gtattcttct tcaacacctt caaagatata atcccttgcg ctttcaaatc tgtcggcaga
     1021 aaccattgac cagtaaccgg gagttgtacc gtagcagact ctcataacgt ctggagnnnn
     1081 nnnnnnactt ctttgaggag tgtaagcgga agtgattcgt caagattctg atttgcaaag
     1141 aagtcaacag catcggcatc aaaatgcatt tcgccgttaa aaagtgaatt tgtgacctct
     1201 gttccgaagc aaatcggatt ataaagaaca atgtcgtcaa tgtcctccca gccgtattca
     1261 acaagataag cagagaggat cgtcgtaccg aggcaccttg caagaacctt gatacgcgaa
     1321 tggcccgtaa cctctttgac cgcctgaata tattcatgaa ggtcatctgc aattgagcaa
     1381 gggtcaaggc ggcaatcata ctgatagaag tatttataca attcatttgt tgcgccctcg
     1441 ggcgttgtta cgtcattgga cggcttgtgg atatcatata taggattgtt tttccaatag
     1501 tctgctgttt tatatcctga attgttcgca atttcaccgt tattgtcaag ggcgaaatcc
     1561 ttgtaggctt ctgccatata ctttgtgaaa agcgctttaa attccgaaaa atcgttcgtc
     1621 agatagccct ttgtatagac aggaacaagc tccttcaaag cattctcaac aaatccgtca
     1681 ggaacatacg gcagtgtctc actgctcggg tcatccttat cggtaaggat tgtcgcacgg
     1741 ccacgaacat agattatcgg taccgtgtca catttacagg tatcttcagc cattgcactc
     1801 ataggagcga tgatcattac gaagattacc aggcttagaa atactgctaa aaactttttc
     1861 aaaactatct ctccttattc aattttatat atgaaatcct gtcttaccgc cgcaattatt
     1921 tttcttcctt tgcggcaagc tcgtcaagat actcatcata tgtgatgttc ttatcgtaga
     1981 aactgcctgt tgtgacatca attattctgt cggctatcgt ttgcacaaac tgatggtcat
     2041 gcgatgtaaa aagaacagtc tccggaaaat tgataatgcc cttgttaagc gattcgatcg
     2101 actcaaggtc aaggtggttc gtcggctcat cgannnnnnn nnncaaggtc ggaatatctt
     2161 cttacccagt cgataagcga atcctcgcag ccatcgaaga actccgagtt gtccgacggg
     2221 aaatatgact gcgacgttgt aactccccac ttaaaagtac cctcgtcggg ttcaagctca
     2281 ccgacaagaa ttttgaaaag tgttgttttt gcaagtccgt ccgtgccgac aaaagcaacc
     2341 ttttcacggg gctttattgt aaacgaaaca ttgtcaagaa cttttctgtc tccgattgtt
     2401 ttgctgattc ccgtaacagt aagcagctca ttgcccacct cacggtccgg tttaaatgaa
     2461 acaaaaggaa aacggcgtga cgaaaccggc atatcctcaa tattaagctc atcaagaagc
     2521 ttcttgcggc tcgttgcctg ctttgatttt gaagcgtttg cgctgaatcg tgcaataaat
     2581 tcctgcaatt cttttttctt ctgttcagcc tttttgttct gctctctgag ctgacgctgt
     2641 gcaatctggg tgtagtcata ccagaagtca tagttgccga catacatttc aatcttaccg
     2701 aaatctatat cgacaatatg tgtgcaaacc ttgttgagga aatgtcggtc atgcgaaaca
     2761 acgataaccg tgctttcaag gtcgataagg aaatcctcaa gccatcgaat agccgcaaca
     2821 tcaaggtgat tggtcggctc gtccatcaga agaatatcgg ggtttccgaa aagagcctgt
     2881 gcaagaagaa ccttgacctt gatatcgtta tcaagctcac tcatcttggc atagtgaaat
     2941 tcatttgtaa ctccgagtcc gttgagaagg atttccgcat cgctctctgc gctccagccg
     3001 cccatctcgg caaactcttc ctcaagctca ctgactttga tgccgtcctc ctccgagaag
     3061 tcctctttca tgtagagggc ttccttttca tccattattt cgcaaaggtg aggatttccc
     3121 ataagcaccg tgcgaataac gtcgtactcg tcaaaggcaa agtgatcctg tctcaggacg
     3181 gacattcttt cacccggggt gataatgacc tcgcccttgt tcggttcgat ctcgcccgaa
     3241 aggattttta aaaaagtcga ttttccggcg ccgtttgcgc cgataagtcc gtagcagtta
     3301 ccgtcggtaa atttcagatc cgctccctta aaaagctctc tgccgccgta ctgcaagcta
     3361 acattaactg tgctgatcaa ttgtatttct cccgaatcta tttatttatc ttttcaaaag
     3421 atattttaca aatttataaa ccttgctttt gaatccgtaa taagtttttc ttaaaagacg
     3481 gatagcaggt aaaaatttaa catacatcag ataatcgcga tcggttacat tatgcacctt
     3541 gccgttccgc tcaaagcttt tcagaacacc gattatctgg tcgttacgaa tattatactc
     3601 attgtcccat tgattgtctc cgcgcataac atatgttccc gaattgataa acataacacg
     3661 gtgaagcaca aatcggccgt tatcccgctg ataaaaaaca atgtcattcg gtttaattgc
     3721 ctttctcggc ttaatcagcg taatcgtatc ctttctgtcc ctgataagcg gcagcatact
     3781 gcgacccgcc gagacgaaag agacctcacc gttactgtca agcgcgtcgc gaataacggg
     3841 tgccatatcg ttaaggctta ttttctcact caagtatatc ggctcctttt aaggcttcaa
     3901 taaattccgt aacatcaaat ctaatatcat cgggctgaac cttgcattcc tttgcaagct
     3961 cagaaacgat ttcctcggtt tctgcgccct tttcgagcat gccccatata aaagtgccgg
     4021 tctcattaac agtaatcaaa cctgtaaaat ttattccgcc gggcgcaaca acaacactct
     4081 tgctgccgac ctttcttatt ctgtagccct ctttaatttt catcgctttt acctccgggg
     4141 gtttccgtaa tgcaaatctt cctgtcctct attcttactt ccttgttgca aacattcaga
     4201 gcggcaggct tgtgagttat tataatacat gttctgtttt caagacctct gagattttta
     4261 agcaggcgtt cctccgttgc ttcgtcgagt gcagatgttg cctcgtcaag caaaagaatg
     4321 ggtgcgtcat ataaaattgc tctcgcaatt gcaatgcgct gtatctgacc ttcggaaagt
     4381 ccgagtcctc tctcacctac caccgtttcc gttccttcgg gaagctgcga aacaaattcg
     4441 tcggagcagc tcaattgaac cgcacgtttg atttcttcgt cgcttttttc gtcacacata
     4501 aaggtgatat tttctctcaa cgtgcccgaa agcagcatat tgccttgcgg aacataggaa
     4561 aacagctttc tcgtatttct gtcaactgtt ctttcggagt catcggtaac gacccttatc
     4621 gttccttttt ccggcgacat aacgcccaaa agaagcttga gcaaggtgct cttgcctatt
     4681 cccgaaattc ccgttatagc cgcgaaatca cccttgttga ttgtagcgct cgcgtcctca
     4741 aagacataat ccctgttata tttgaatgtt atcgaatcaa attcaattga tttcaacttc
     4801 tcataatatt cataacggtc accgatatcc tcgtcggcat caagctcatc atcgtaattc
     4861 tcaatctcga ttattctttc cgctgacgct ataatcgaat aatatgtcgg aattacgcct
     4921 gccaaagcgg aaagcggcga ctgaatctga ttgacgagct gcagaatagc cgtcaactct
     4981 cctatatcga ttgttccctg atacacacga aatccgccca gcaccattgc caaaagatat
     5041 ccgaggctga acgccatctg aattcccgtg tttgcaaata tgcttatcgt tgctctttta
     5101 atgcgtattc cgtaattatc cgcctggagc ttacgagcct tatcggcaac ctgtttgtaa
     5161 gcgttgaata ccttaattac aagcaaactc gctattgact cctgaaagaa tgagcgaacc
     5221 tttccgtcag cctcctgtgc ttttttgtga attcccttta aatagtttct gaaaaaagcg
     5281 gtcgtcagcg cgatcattcc gcctcctgca agaaaaatca cggcaaaaat ccagtcgaga
     5341 cctatcatta ttatcactgc cgaaataagc tttgtcaaaa gcgaaacaac atttggcacc
     5401 aaacccgtaa ctccgtcaca aatcgtttgg acgtcggagg tcaagcggtt aagcagatct
     5461 ccgctatggt gctttgaaat ctcggaataa tcctttttca ttatggattc aaaaacactt
     5521 gttctgattt ttatctcgat ttttgcacgg gcacgctcat acatattgct gttgaaaatt
     5581 ttcagtgtga tttgtataag aacgagaatc aaaagataaa tcgcaccgtg tttgagcagt
     5641 tcaaaatccg cacctgttgc accgtttacg atatccttgg tgccatatgc ggtcaatatt
     5701 ccgaaatatg caagtgcggc gctcaaaaca gcatacaaaa agacaaaaag ccaactgcct
     5761 tttgatgtct taattatcca tttaagagca gaaatatcgc cgccgttttt acaagcttga
     5821 ccttttggtt ttttgtcttt caaaatcatc tatccttttt catcacaaca gtctttgact
     5881 cattgttctg tatgaaagct ctgccgcttc aagacttata ttacatttaa gtctgtaaat
     5941 agggacttcg gcaagcagtt cattgagaaa gtcgcaaaga agaatcattc tctcggggtt
     6001 tgccggtcgt atcgtctgtg aaataagagg actgagcgca tcatcgggat caacacgttc
     6061 tatcgaattt gtcgggcttt gctcaataaa gcagattgcg ccaagctcgg cagaggtttt
     6121 gacgttgata tcgtttttac ccgaaaacgg cgtgccaaag atataatact taccgtcaat
     6181 cttcctgtat gccgccttat cgtcattgat tataaacgcc ttatcgccca aaagcttcag
     6241 ccaaagagaa gtgtgcgtag acttgcccgt tgagcacggc gcggaaaaag aatatgcctt
     6301 accatctgcg acaacggttg aagaatgaag aaacataccg ttatattcaa gcaacttgag
     6361 attaaatgca gagcctaccc aaagatattc gaggctttcg ccctccaaaa catccttata
     6421 atactccatt tgccgttcca tcaggtcatc actgaccccg attttaaaat cggctttttc
     6481 atcacagcga tatttttcgc ttcgctctct tagcaaatca aatctcggtt cataaccgac
     6541 gtttagccct gcaattttat aaacaggcat ccgttcacct ctttcaggtc ttattcacgt
     6601 ctaccataat tatgataaat atgaataaaa tctaagttcg gcagacaacc gtaaaggctg
     6661 tctgccgact cggtcttttt gaaaattagc caatgatgag atcgctgccg atgctctcgc
     6721 ctgctgactt tgcgcgtgtg tcaacagcgg gatctgtcgg cggagtcact gctgaacttc
     6781 cgtcagccat gatgtcctca acaatatcaa attttctgat atcaagctca ggcttattgt
     6841 aggtcatttt ttcacccctc gtaattatac tcgacacggt gagcaaaaaa ttaaacaaga
     6901 attctttacg ttaacattta atttttcact actccaatat ctatgttaat tctatcattt
     6961 atttacttgt atgtcaatat atttttctgc aaacgaacga aattaaatca caaaagacaa
     7021 agctccgtgc cgaagcacgg agccaaatta gcattgtctt taaagaatta gaacacacct
     7081 ctacctgtga tataatcacc tgtaagagca aatgttgtat ttgcggcacg tgttctcaag
     7141 ccaaggttga acttgccaat atagaaatta accttaatag tccatacaag ctcgttacca
     7201 tcctcaacag gagtccatgt aacctgatca tatgtacgtg tatttccgtc cggatcaata
     7261 agctgaagct taaatacatc tttgcttgtt ctgatagtta catcatgaac acctgcatag
     7321 atcttaccgt ccatatatgt tcctgcagga tacatatcgg gaataaggat tgaatgaata
     7381 ctgagatcaa gtccctctgt tgcattgatc tcgagagcaa gacctgcatc gttgtattcg
     7441 tctccccatt tgccgaatac gttgtatgaa gaatgagctg ttgtagcagc aaccttgatg
     7501 ttccatactt cgttatcgcc gtttgttgtg ataacagcgt cttcacggtt aaatgtaaga
     7561 gtgttttcgc cgtcgcctac aagacggata gccttcggag aaccgttagc tactacctga
     7621 acgttagcgt ttgaaccgat tgtcggtgtt ccgataacat cagcgcttac aagcttgttg
     7681 agaacaagag ctgccttagc gtctgtgatg ctctttgttg catcgtcaac tgttgtctgc
     7741 ttatccttag taaggtctct tgctacgcgt gaacctgctg ttactgcagc ggcatatgcg
     7801 ttccatgtgg aagcggtgta aagacctgaa actgagctgt caaagccggc aatagctgcg
     7861 tcaagtgctg tgtagtcagc gtactcaacc tgtgcgcctg caactgttac tcttgtacct
     7921 gtgatatcaa gagtcttatc gaactgatat gattcatctg caagatagca aaggcaagca
     7981 tttgaatcct tgtttgaacg atagaactgc cagcacggtg cgattggctt attctcagcc
     8041 tcacctgcgt catacacgtc aagcttattg aaagtgtaca ttgcatcatt ggggcagaaa
     8101 atatctgagt atgtgcccgg ttttgcggta tcttttacct taaacttgta tgtgataatc
     8161 tttgtgttat cgcacttaag gctcttaacg tctgtaagct catccttcgg tctgattgaa
     8221 aaacgatagc cctcatactt gtcatagcag ccttcttctg ctctgaggct gtcaggccac
     8281 tcaacgttac gcatgtcgcc gtatgctgcg aggtcgtaat cgccgtagtg cttaccttca
     8341 atatagtccc attcgccgag ctcttcgcca agagaatgaa ccttgatttc atctgcagta
     8401 tccggtgtgc ctctgtgaga ctcgctgcaa cctacgatat caacgactga gttatcatag
     8461 aaaacagttg ctgtcatgat acagaatgtg aaatcggttg taagatagag atcaactgtg
     8521 aactcatcac ccggattaag gacatccttg cttgcaacaa actttactgt tgccttgccc
     8581 tttgaaagat ccttgtactc ataccagaag tttccgtatg cgtcaaagtc gttgttgtgc
     8641 ggtgtcttca tcttacgata gagagcgccc cactctgcat ctgtagcggc atcgcccttt
     8701 gcttcaagag catgatactc gtcgatgatt tcatccttcc aatagtaaag attgtcttcg
     8761 ataacatctt tgcttgctgc cgtgtcctgg attatgtagc cgaatttctt gccggaaagc
     8821 aatgtgcggt caattcgatc cttcggatcg gcaatagcaa gttcttcttc aggataaagt
     8881 tctctgtctt ctgccgttat tgtgtcgccg gcaaaactgc aaattgcaaa agccgagaac
     8941 gcaaaaataa cagaaagaac aatgctgata attttctttg ccatgaatat tcctccttta
     9001 aaatatctta atccatccgt aatggaatta agcccgatta ggcaagctta cctgtctttt
     9061 gattgatgga agcttgtttt ctgacaacag ctctcaatgc aagatagtcc gcattggtca
     9121 gcgcctttct tgaaaccagt cgtgccgcct tgcttgccgc aggcgaaaga atgaagttct
     9181 tctgaatggt attggaaacc ataagctcat cgaccgagtt gattacgccg tcgccgctga
     9241 catcaccgta aataataatt gtatatgttc cgataacctc acctgtaaga gttgatgttg
     9301 ccgtcacaac ggttcccgtt ccgagatatc tgcccttgtt gccggaatag ctgattgtaa
     9361 cgttttcata tgttacgaag ttattcagga acgaagcctt tgtcaaaccg gtagtcaagc
     9421 cgtagatata accgtctttg attaccgttg tgctaccgtc tttagcctta aagattgcct
     9481 tagcatcctc aagctcgaga gaatcaatat agccctgcaa agttgcggtc atttcatcaa
     9541 cctgcgactg cttatcaatc ggcagattca tattattctt cacggtttct tcacgataag
     9601 cattgatgag agccattacc tcatcaaaat tcttatatgt attattataa atttctgagc
     9661 tcgaattgtc aagcaaatcc aagatttttt gaagttctgt atagtttgcc aattttttga
     9721 ccaaatcctt ggtagctttg acgatagttt ccttatacga gtcaacctcg ccctgtctgt
     9781 caaccgtcaa acctaacgga atattgtcga gaacctcctg cagcgctgca acgctctcat
     9841 cggtatacca gccggtttcg atttctgtac gtgccgcagc agcagcttcc tcaagctcag
     9901 tgtaatctgc accgatatat ttaaggtcaa gcaatgcctg ctcgattgct tcggcatagc
     9961 ctgtaacctg ctcctgatat ctgatatcaa gaccttcttc aacagcattg agcttattct
    10021 gaagattttg ccagctttct tctgtgtaaa gtgatgaatt cgtgggaacc ttgcccttag
    10081 cggtttcaac tgccgagtaa tctgccggct tgtacttcaa gccactgatt ccggttctga
    10141 ccgcctgctc atacatatcg acctttttct gatttctgat atcaagattc ttgtcgattg
    10201 atttaagcac gtcgttgaga gaagcaaccg tttcgtcggt ataatatccg gtcttgattg
    10261 ccgcttcggc atccgcaata gcttcattca gcttcgtata atctgccgga agatatttga
    10321 ggtcggttat cgcctgctcg attgccttgg cgtaattgtt gacctgctcc tgatatctga
    10381 tatcaaggcc ttcctcaact gcactaatct tatcctgtag attcttccag ctctccgctg
    10441 tataaagtga cgaatccgtc ggaatcttgc ccttggctgt tgttaccgca ctgtaatccg
    10501 ccgggttata ttcaagctcc gcaacagcct ttctgattgc cgctgcataa ccgtcaaccg
    10561 ttgcctgctc ggtaacatca aggttatagt taacaagatt gatagcgttt cttaccgccg
    10621 ctcttgacgc tgcggtgtgg agcttttcat ccatcgctgc atttgcttct tcaatagcct
    10681 ttcttacatc atcataattt gcaccgatat aatcaaggtc agcaatagca tcttcaatat
    10741 tctgagcgaa tttatcaacc tcggcctggt gagtaatatc aaggccttct ttgacggcat
    10801 tgagcatatc ctgaaggtgc tgccagctct cctcggtgta aagtgaggaa tctgtcggca
    10861 ctttagcttt cgccgcctca acagcactgt aatcagcatc cttatatgta agtcctgcga
    10921 ttgctgcacg gattgccggc ggatatgtat ctaccgttga ctgctgagtg atgtcaagct
    10981 catagtttat tccgttgaga acctcattga gatttgcaac ggtttcatca gtgtaaagcg
    11041 agagatccga aggaatttct cccttagccg ttgtaacctc gctgtaatca gccggaagaa
    11101 ccttaagagc tactatagca tcctctatat ccttagcgaa agcatcaact ctgctctgct
    11161 gagtaatgtc aagaccttca acgaccgcgt taagcttatt ctgaagattc gtccaacttt
    11221 ctgtcgtgta aatgcttgaa tcggcaggaa ccttaccctt agcgacttca accttgccgt
    11281 aatcagccgg cttgtattta agtccctctc ttgcagcctt gattgcggct gcatagccgt
    11341 caaccgtcgc ctgctgcggc ttgccgagag ttctgtcaac agcattgacc gcatcattaa
    11401 gagcctgtac cgtttcatcc gtgtaaaccg taaggtctga cggtatcttg gcaatttcgc
    11461 tatcaacctg tgtgtagtcg gcagggataa atttaacctc aagctttgaa attgcatcat
    11521 tgattgcggt cgcgtaaccg tcaacctgtg cctgtttcgt tatatcgaga ttgtaatcga
    11581 ccgcatttat cacattattg agattagcca ccgattcatc ggtataaatg ccctctgcga
    11641 tttttgcatt tgccgcatta acagccgcaa tgacagccgt gtaatcagcg ccctttggtt
    11701 tgagtgcgat aattttagct ctgatatcgg aagcgtaacc gtcaaccgtt ttctgatatc
    11761 tgatatcaag atccttattc attccgttca ccgtatcatt gagagcctga acggattcat
    11821 ctgtataaat tgtaaggtct gccggaattt ctgagatagc attgtttact tcggtataat
    11881 cggcaggctt atatttcaaa accattatcg ccgtttcgat agcctcggca tattggtcaa
    11941 cctgattctg atatctgata tcaaggcctt cctcaacttc attcagctta tcctgaagat
    12001 gctgccagct ctcctcggta taaagtgacg aatcgctcgg aaccttagcc cttgctacgg
    12061 taacctttga ataatctgca tccttaaatt taaggttgct gatagcgttg tttatgctcg
    12121 ttacataacc gtcaaccttt gactgatggt ctgcagtgag ggtcgtgtct atctttttaa
    12181 gcacggaatt aagcgttgct acagaaagag tggtataaag cgaaagatcc gtcggtatat
    12241 ttgtctttgc aaggtcgacc tgtgtgtaat ctgcgggtgc atacttcaga ccgattgttg
    12301 cgtctgatat tgccgtggca tatccgtcaa cagttttctg ctgggttata ttaaggcttc
    12361 tgtcaacgcc tgcgattgca aggttgagag ccgacagcgt ttcgggcgtg taaagagtgt
    12421 agtccgggtg tgcacttgca tatgcctcgt tttcagcaaa ctttgcattg gcttccgtta
    12481 ttctttgatt aacaacggca taattagccg gaagatattc aagccctccg atggctgttc
    12541 tgatagcctc ggcatagccg tcaacatcgg cctgcctgct gatgttgagg tttggcacaa
    12601 ctgccatata agcatccctc actcttttaa ggctttcagc cgtatagagc gagcttccgt
    12661 ttgtgattat cggagttgct tcctcctgcg cagaggtaac agccgtataa tcggccttgt
    12721 tttccttccg cgccgatatt gcatttctga tagctgtagc atagccgtca acctgttcct
    12781 gcttgctgcc gtcaagattt ctgtcgacac tgtttattgc cgcagtgaca tccgaccagt
    12841 ttgaatagaa ggtatcagcc gtaaatccgt atgttgaggc ataggtttga agaatattat
    12901 ttgcaatctg attcgcttcc tcaatcgcct tgtcaacctc cgtataatcg gctctgtttg
    12961 ctataagtgt atcaattgcc gatctgacag cggaaaccgc tctgtcaatt tcgctctggt
    13021 atcttatgtc aagaccgagc ttcgattcaa gagtcgcaac tgccgaatca acagccgcaa
    13081 tattgctgta ctttgttctg tcaattgcat tggcctctgc aataatattg attgcctcgg
    13141 ttgtatttgc aggcttgtat tcaatattat ccagccaata cttgagtgtt gcgactgcgg
    13201 cttcgatatc attctgtctt gagagatcca aattgtgaag cttaataagg tcattataag
    13261 cctttgtaag gttttcagcc acatcatcaa caatatatga aagattgagc agcttatatg
    13321 cgctttccgc cttcatggcc tcggaataat ccgcaccgtt gagtttcaag gcgtctatcg
    13381 cgtttttaag aagaaacgta tatccgtcaa ccgttgactg ttcggtaata agaaggtaag
    13441 tgccaacggt atagccctca aggtcattat atacacaacc tgaattttca aggacggata
    13501 ctacctttgc ccagcccgaa tatgcctcgg cggcgctctt tccgtatatt gtcggcgttt
    13561 cgttcaaaaa tgtattaact gtgttaacat tggcaagaaa ctcggtataa tcggcagttg
    13621 tgtactcaag cgcatccatt gccgccttaa tatccatcgc cattttatcg actgcaggct
    13681 ggtaaagtgc gctgcaatca tcggcatagc tgtcaataag agtttgaagc tttgcccatg
    13741 aggcaagagt atatttgctt tcgttctttg aaattgcctg cttataataa gcgtctgcga
    13801 gtgaataatc cgccttgcgc atttgcagat ttgcataagc cgtctgcaac gttgcatatg
    13861 ccgaatcaat atctgtctga gtttcattgg gctttgcaag gcatttcttt gcgtttacaa
    13921 aagcgttttt gaaatcctcc cagcccgatg aataatacca tgactgagga ttatagccct
    13981 taaattctcc gtcggcaaga tttattgaca tcgttgtcgg atccgttccc tgaatttctt
    14041 caacaagagt tctcagagag cctttatcat atgtaacaaa tctgaagctg aatgaatgac
    14101 cgacataaac tttcgtccat gctgccgggg tgtgatactt taaggaaaca gtatattcct
    14161 tcgttcctgt ttccttacac ggacctacgc cctcaaaata cgactgcata gtcgtactta
    14221 aagctgacgt tgttatatcc gttttattgg ttggcaaggt tatgccaagc tcagaagcag
    14281 cagttgtttc attactcgga ttgtttgatg tgtcgtcggc actgaatgtg tctgcgatac
    14341 ccgaaaccgt atatgaactg tcaactttca cggtaacgcg ctcttttgtt gcagccgtat
    14401 atgtcggaag gagggtatga aagcgaagat taagagaaga aagagtagac gtaacactct
    14461 tgtcaatgta aacagttgac agaggtctgt tgctgtccgc ttttattgag acattataat
    14521 ttttgcttgc acttgatccg ccgaaattct tcatattgcc aaaattttta gtccaagccg
    14581 aaacctcgct tgtaaacgca cttgcattgg tgaaatcaac cgtaccgttg gcggttccgt
    14641 tgttataaat tgaaccgtat gtattctcgc cgagaatcat cgttgcaatg tatgaacggt
    14701 tcttggtatc cgtaccaaaa ccatagtctt cataggttct cttggttgaa taaatacccg
    14761 ttggggcagc tatcgcttca acgtaagaca cgcattgagt ctcatagtta ttggtatagg
    14821 ttttgccgct tatttcattc gtctcgctgt atgtatagct gacggtgaag ataagaacag
    14881 aacccgcctc ggcagtacct ccgctgacgg tccatgtgta tgtaccgtta gaaaatgcgg
    14941 gcgtagacga agcataagtg accgtagtgt tgttgcacga aatcgtcggt gcactgtcaa
    15001 gagtaactcc cggtgtaaaa gaaatctgcg ttgcatacgg cgtttctcct acatatgcaa
    15061 tattggcaaa gttaccggac agataaggaa cacctgactt tgttgcctta actatcaccg
    15121 aattgttccc cgaaggaggc ttgagagagt tgcttgcata agcgactctt gtaacctcgt
    15181 tgacataaat gcctgcggtc ggtctgttaa gaacaagctt cgggtcggca ggagccgccg
    15241 cagccgtgaa tccacccggc atcagagtca gcggaacaga tgaaagaacc aaggcgatca
    15301 ccataactat gctgatgacc tttcttttca gtttcatcgc aaacaccacc ttatatttat
    15361 taaaatgttt tacaaaatat atccgttgtt taaatcagat ttcccgaata tacctaagtt
    15421 tattttaaca catgatatac ttccagtcaa taaaattaaa tgtattttat atttttgaca
    15481 aaaaaattat aatttttttt gctaactttc ttgtgcaata tgttatataa aacaaaatac
    15541 accgtatgac tttattgaaa tcggacattt tgttcaatta ttgttctttt attagaacaa
    15601 agtctccttt tagaaatcaa acttgtttat tctctcaaca aaacggatca ctttgttttt
    15661 ttgttacagt tatcgcttta tcctgacaaa tcaagtttct ctttcttttt tcatttttta
    15721 acctcgattt tgctttttta tcgcataatg acagtgatca ctccgtcgaa ggcagagttt
    15781 tttcggtttt gacaagtctg ttgtttacaa ttaccgaata tacaagcgaa acatcaaaaa
    15841 tgaggctgcc gttttaccgg cagcctctgt atttttaaat tttattttgc cgcattggca
    15901 agatcgatga atgatgtaaa cttaccgatg cactccgaag gagactgatt gattgtctcc
    15961 ttgcgtacgc ctgcattttc aaccgactta acatcggagt taccgatatt gccgtcaaag
    16021 ttgaggtcgc ccgcatactt cgtatagtcg gcaatcaaat cgggatcaag cataagtgat
    16081 gcgtaagcct tgagaacaac tgcatcctgt ccgtcaatct ttccgtctcc gttaacatca
    16141 ccgaacataa tgagcgtaaa ctcgttcttt acagtgtatt ctccgtacgg atcctcaacg
    16201 atctggagct tggagcctgt tcccgggccg tgcttggtag ccttgaggca gatatcaccg
    16261 tctccggagt atccaaactc acttgagaaa tagtagctga aggattcttc gccgtaggtt
    16321 tccttagcag tatcgttcca gaatgtatcg tcaaatccga ttgcgtatcc gtaacgatag
    16381 tcaatgttct gattaaatgc cgttgagccg ccgttgtaga actcaatcgt tgcaacgttc
    16441 tcaagctttt cgattgcatc gtaaagtctc ttatagtaat tgtcaacaag cttctgactg
    16501 tccgcatcat agt
//
LOCUS       DAWBSW010000021        16352 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_789_length_16352_cov_2.871449, whole genome shotgun sequence.
ACCESSION   DAWBSW010000021 DAWBSW010000000
VERSION     DAWBSW010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 16352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16352
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_789_length_16352_cov_2.871449"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3577..3586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4469..4478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5184..5193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11364..11373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13337..13346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4574 a         3665 c         3417 g         4646 t
ORIGIN      
        1 atgcttttca gctccgtcgg caccgagttt ttgagcgtaa ctcaaagcgt caaaatcatc
       61 tgcttttctg tagctctcgg tgtcaattcc gttataaata agcgttgatt ttctctcgcc
      121 gtaaaaatat tccgatgcaa catccgaaca ggccaaaagc tctgttgacg agcgaataat
      181 aagacctttc atagtgcggc tgtagatttt ttgtaaaagc ttcttcaaag ccgccaatct
      241 gccgacggga tcatactgac tttttgaatt gtgagcatgg caaattctca ttggaattcc
      301 gcattttttc gcaattgaca gcgttattcc gttgagaaga tccatatttg aatgggcaat
      361 gtcaaaatgc tccgccgaaa gaattttctt gacatttttg atataatcaa gcttcttttt
      421 cggtgcatca agatcgcctg catggataac tcttatgccc atatcggcaa ctgtcttttc
      481 gtgaaggcaa taaacgccct cgtcaatcgc aattatcacc gttatatcat attttgtttt
      541 gtcgattttc ctgagcacat tcacaaggaa ggtttcgatt ccgtttgcgc tcagtccgtg
      601 tgaaaaaaat acgatttttt tcatgtcaac acctgccggc tgttatttgg aagcttcttc
      661 gtgtcttagg gaaaccgtgg caagaatttc tctcttgaaa tcgccgtaga ttttgtatct
      721 ctttgagaaa acgatgaatg aaataatgat aagcaccgga ggaacaatga acataagcgc
      781 cgagatagcg tccttagcct cctgcgtctg ctcgggagca gttccctcat actttgttat
      841 gctgaacgtc agatacatga taatacactg aaccgtatat gccattttct gcaaaaagcc
      901 cttcatcgag aatgtcaagc tctgatttct cttgccgagc ttatattcgc cgtaatcaac
      961 aacatcggca agcataacgg tctgattgac gaacatactt ccgataccga ttgatgttat
     1021 aagatagcac gcaccgagtc cgtaaacgtt gccgccgaag ccgtagccga tcacagccat
     1081 gccgatatat ccgactgccg ccatgccgag cgaaagctta tagatatttc tgttgttcat
     1141 attaagcttt gaacatatcg gaataacggc aataccgatt accgagccga ctgctccgaa
     1201 catattgaac tttgactgaa ggttgaggtc tcctgcaacc gagttgaaat aatagctgct
     1261 gatacccgag gtcatataat agcctgcgtt tgagatcatg gcaaacagca taaatgtcaa
     1321 aagctgatcg ttgttcttta caacgtttat agcctgctta aacgaaaact tctcgccgct
     1381 ttgaattaca tttctttcct ttgtcgaggc gacgctgatg catgcaaaaa caacaagcat
     1441 gacggcggca atgattgacc attttccaaa gcctgttcta aggcactccg gcgtcattgc
     1501 tttgagactg ctctcgcttg aaccgccgag atatgcaaca ataggcgccg taagaataag
     1561 cacgattccc tgacccagac cggagaaggt tcttgcaacc gtagaaataa agtttctgtc
     1621 cttttcctca gttgcaaacg acggaatcat tgaccagtaa ggtatatccg ccaaggtatt
     1681 tgacatgccc caaagcacat agaacaccgc aacataaaca tacatccaaa ccgaatctgt
     1741 ttttattccc ggattattaa aaagcaggca gaggacgact gcgtttgcta ttgcacctct
     1801 gagtatccac ggacgatact tacccatttt gctgtttgac atatcaacaa gagttcccat
     1861 catagggtca ttgataccgt cccacaatct cgcgatcggc gcaagaatga gcagaaaaag
     1921 cgagctgata ccgagcgcat ttgtaagata aagcgaaagg taagaataga ataagccata
     1981 gctgagatca agaccgaccg cacctacgcc gtagccaagc ttctctctta aaggaacttt
     2041 tttatccatt ttgataccta ctttcataaa ttagcctaaa ccaacttata taataccaca
     2101 aaaaaacatt ttagacaata gaaaaaatga cgttccgcaa gaaaaaggaa cgtcattttc
     2161 ggtttattat ataattatta cagtctaacc aatccgacag caattctgag aatcgtaagt
     2221 gcatccatgg aattaacctt gttgtcaccg tttgtatcgg cattcataaa tttctcatcg
     2281 gtgtttatct cactcgcgag accgaccgca tggcggagaa caatcaatgc gtctgccgag
     2341 ttaataagct tatccccgtt aacatcggga tatgcctttg cgattatctc tgtctttgcc
     2401 ctcgccttac aaaccttgca aagcatatat tttgatccgg tcgtgctgat cgtcggtctc
     2461 ttttcgatta cccattttcc gtcgccggta tgtcccgttg ccggaataac ctcattggtt
     2521 ttatttccgc aaagattaca ggtaactata tagtttccgg cccttgtgca ggtcgccgcc
     2581 ttgctgacct tctttgttac atttttatgt ttacatacaa tcttaaactg cttaacataa
     2641 ccctcgccgt atgaatttat acctacgata taagcttttg ccgttcctat ttcgatattg
     2701 ttacgacaaa caaccttata gtccttgccc aaaacaactt tttcaccgct tgtaagcgtg
     2761 attgtaacgg caggaattac ctcagatttt gtatattcct gtccggcaat attggcaacc
     2821 gtaccgtggg aaaggtcctt aacaatttca accgatgctt tgattccgct tgatttgata
     2881 taattattaa gtcttgtgtt gtttgccacg gaaataaatt tcaaattgtc ggagcaggcc
     2941 ttaaacgcat ttgctcctac ggtcgtattc tttgaaatct taaccgtatt aagtaaggta
     3001 cagttttcaa atgcaccctt accgagagtc ttaagcgtaa acggaagctc aacagatgtc
     3061 aggaacgggc agccggaaaa agcgtttaca cctattgtct ccagacccaa aggcaagctg
     3121 aggcttttaa ggccgtcgca ctgtctgaaa gcaccctcat aaattgaagt cacattatcc
     3181 ggaataacca gctcggtaat tcccgaacag cccatgaagc tttcttttcc gatatattca
     3241 agagttgacg gaagaacaag cttttttatc tcggcatgat taagaaatgc agcataatca
     3301 atcccgacaa tagggagtcc cttatatgta tccgaaagaa taacggtatc ataagttgca
     3361 caagtataat ccgtaataat ataacccgtg cctgccttat taagcttaca gtcaatatca
     3421 ttcattgaat tgcgaagaat ggtaaatgat gcacccgaaa cattcacatc gttcgtcatg
     3481 ttaacaaaac cggagtttac ggcattgttg attttcagac tcaacttgct gcttccgtga
     3541 tttgcatagc ttttgacctt gaaaacaatt gtagagnnnn nnnnnnattc ctgagaactc
     3601 atagttattt cctcccgcaa ggctccagtt gatttttccg ggatcacctg catattcgga
     3661 ataaacctca ccgtttgtct gcttatccct tgctcttcta agatccttgc cctgcttcat
     3721 actgacaaac tcaagctttt gagaatcata taaaaggctc atgtcgagag acgagatctt
     3781 tttatatgcc ctcggaaaat aaacttcggc ggtaatctga tcgtcctggg aatatgaggt
     3841 cgcatcaatt cttatagaaa tatcagcaga tgccgctgac ggagaaatag ccattgcaca
     3901 gaaaatcagc acaaagacag ccgtaaccgc aacaatcctt ctgaataatc ctttcattct
     3961 ttccaccctt tcaagtatat aattgccata gtatttatta tattttatat tatttaaata
     4021 gaattgtcaa ttgctaaaat ataaagatta cttaacctat atttttttgc ttgaagaata
     4081 catatctttg tgatataata ttattgtttc cgcccgtagc ataattggat aatgcatcag
     4141 attccgattc tgacgactgg gggttcaaat cccttcgggc gggccataaa aataagaacg
     4201 gagccatgct aaattgcaca gctccgtatt attttatttc aattttacct caatgaaaag
     4261 ccctcacggc gaaccggcaa aacctctttt tcgaggttgc cgccgttgct gagataaatc
     4321 ccgtaaagct cctgctgacg ttcatccgaa taggtatatc cccactgatt ctttatcttt
     4381 gcaaaatcct caaaggtctc gtcaggcagg gtagtgatcc tgtcaatatc aagcggaagc
     4441 ggaatacaaa ccgtgccgtc agaggttann nnnnnnnnct cgataagtct cctgtttgcc
     4501 tcctgctttg gatattcctc gtcaaacata attcttgtaa tcgggaatac ggcgaccttt
     4561 gcgccgataa gctcatatgc atgcgtcgaa agccccgaat gtccgtgatg tgcgacctga
     4621 acaacgtcgc atttcagttc attattgtag cgtgcttcaa gcatcttgcc tgcaagggcg
     4681 gagcagtcgc cggggataaa caccttcgtt ccgccgacgc tcatcatcaa agcacatgag
     4741 gaatcgttat aatccggcat cttcttggga aagatatcct catgggtgca aagcacattg
     4801 aagctgagat ttctcaccgt gaagcgcata ccggaatgaa gcttatactt aggaatcgaa
     4861 agcgcatcaa gctgtctgaa aagcttctga gaaagaagca cttcttcaat atcccattcg
     4921 ttatcaacgt catacttggg gtcaataaga tttgaataga atccttcaat aataacgtca
     4981 tcacagttat agcgaagaaa atccatcatt ttagagatat gatcggaatg agcgtgagag
     5041 ataaaccagc cgttgacaac aatttttctg tccttcggcg ttctgtcacg cataaattta
     5101 tgaattctgt cattgtcgtt gaactggaaa tagtgaccgc tgtccacaac aaaaaagctg
     5161 taatcggggc actgaataag caannnnnnn nnngcacatt ccgcagtcga tatatgtgtg
     5221 gtcattctcg aagccgtaga acactgtttc gcagtctccg tcgcacacag gctcgccgtc
     5281 ctcggggaca gggttctccc ctaccgtcac tctgagcgag ctgtcgcatg gcgtataata
     5341 tgacgtgatg tgcaagccgt ccttttcgaa catggcgaaa cggttcccgt ctatttcatt
     5401 ctttcttatg gctaaaaagc cgttttcgct gagttttttg agccaagcgt taacttcatc
     5461 aatatccgtc gagcgataaa tcgccatttc gcagttttca cggcaatcgt aaatcggctc
     5521 gtcgacatgc tgcctgaatg cgagatcact ttcaaaaaaa atcattattc ggcgtcctct
     5581 tttttatact tctttgcaat aaccactaca ccgactacga taattgcaag cacaatcaat
     5641 gccgccgcac cgacaattac aatcttcttt ttatcggtta tctttgtgct gttattctcg
     5701 ccgttcttgt ccgatgtcga ttcggcaaca gaagtggttg actctgcgct gagttcggag
     5761 ctgtcggcag tggtcgtaac cgccgaggtt gcggtcgttt tgaccgaagc gtttgtcttg
     5821 tcaacggctg tacccgcctg acctgccgcc gcagaggtct ttatatccgc cgaaccgccg
     5881 atctggataa cctgagcatt cacggaaata acctcatttg cggttgctcc gcttattccg
     5941 caatttgtta ccttgagctt aacgtccttc tctgtcagct tggcagacga tgttttcttc
     6001 agcgtgagca caagaagatc ctttctgttc acagctttga atcctgttat tgatgcaaag
     6061 gtaaacatca tcgggtttgc atccttattg aagcttgtca atacaacgcc gttttcattg
     6121 tcctccgtat aaatcttaaa gtttctgagg cctgcaccgt tcgcactttt ttcaacgctg
     6181 accctgcttg aaagagttac ctcaaaatcg aggcagttaa atgcagttcc tccctcataa
     6241 tcaattgaaa cgacggcttt tgtattgtcc tctgagacaa cattgaccgc aaaggttgcg
     6301 ctctgcgccg caaaagcagg cagacacagg caaaatgcca tcactgcggc aattataatc
     6361 gaaataattt ttttcataaa aacgacctcc gtttataaac ctatttaaac aatatgttat
     6421 atatgtgaca taggctttat taagagtaag tataacacaa aacaatgttt ttttcaaccg
     6481 ttgtatcttt agtcattttg cataagtaaa gattcgttat tttgttaatt tttcaatatt
     6541 gactgattat aatatttcgc atatgatatt cggagggcaa tattatgaaa aaagagagta
     6601 aattcaagct taaccaagtc gttctggcgg cactttttgc ggcacttacg accgtcctga
     6661 cctattacgt caagattccg agcgttaacg gatacatgca catcggcgat tcgatgattt
     6721 atctctgtgc cagcattctc ccgacaccgc ttgccgctct ttcggcaggt atcggtgctg
     6781 ctctttccga cgctgtgggc ggctacacaa tgtacatcat tccgaccttt attatcaagg
     6841 cacttttggt tctctgcttt acgcatacgg ccaagaagct tctttgcaag agaaacatta
     6901 tcgccgtttt cgttgcagcc gcaatcacga tggtcggtta ttatatcgca gaggttatta
     6961 tccttgcact ttcctcaccg gtcggattca cggaatatct tttcagcgtt ccgtcatggg
     7021 tcagcgcact taacactctc cccggcaacg ctattcaggc aggctcgagc ggaatacttt
     7081 atattgtact ttccgccgca cttgacaagg taaaaattaa agacagaata aggaagattt
     7141 aaatcatggc tgatattgtt tacacctacg gcaacagcgt ttatatgaac ataaccaaca
     7201 aatgcccctg ttcctgcgtt ttttgcatca ggagcaacgg cgacggactc ggctcagcag
     7261 aatcgctttg gttaaagaag gatccgacgc ttgaagaaat tgaggatgaa atcaacaagt
     7321 ttgatttttc accgtattcc gaagtcgtat tctgcggtta cggcgaaccg acacaggctc
     7381 ttgataatct catcgcaagc gcaaagtatc tcaaggaaaa gtacggactt aaaattcgtc
     7441 tcaattcaaa cgggctcagc gaccttatca acggcaagga gactgcaaag cttcttgaag
     7501 gcattgttga ttcaatctca atcagcctta acgcaccgaa tgcaaagcgc taccaggaag
     7561 tcagccgttc acgcttcggc gaggatgcat tccctgccct gctgaaattt gccgaggatt
     7621 gtaaaaaata tattccgaaa attaaattca cggtagttga cgtccttaat aaggaagaga
     7681 ttgccgaatg tcagaagatt gcagataaca tgggcattcc gctgagagtc agagagtgga
     7741 ttaaataagt taatatataa ttcaacgggt ggtcgaaaag gccgcccgtt tttatttttg
     7801 gctgcatttc acgaagccta tgtcacgccg tattttcgac atacttttag aatataatac
     7861 tggttcattt tctttaaatc acgtatcaaa ttaaatttaa gtctgatttc ttcaaaaatg
     7921 catagaaacg taatactctt cattttttca cataaaaata aaaagaggct caaaatgaac
     7981 ctcaaaaaag aaaaaccaca caagccgtac gcccataggc ttgtgtgggg gcgtttagaa
     8041 ttccagtaag gatttatccg gaataaatcc taaattctta gattagtctt ccttcttgcg
     8101 aagtgcaact actgctgcac cgctgatagc gagaagtgca actactgctg cagaagcagt
     8161 gagacggtcg cctgtctcag ggttaacaac atcgctgctt gaagcggctg ttgttgtatc
     8221 tgctgccgga gcagttgtaa caacgtcatc agactttgtt gatgatgtaa catcatcagc
     8281 agccttagtt gtagttgtaa cgtcctcagc agccttggtt gtagttgtaa cgtcctcagc
     8341 agccttggtt gttgttgtaa catcttcggc agccttggtt gtcgttgtaa catcagcctt
     8401 cttggttgtc gtagtaacgt cagccttctt ggttgttgtt gttgttgtag ttgttgccgg
     8461 tgccggaatc ttgttgtcaa gagtcggtgt aacaggatta ttgtttccgt ctacagtacc
     8521 gtcgaactca acaatgatgt cactcggttc aatctttgct ttcgcagaat tgccctttgc
     8581 aaaggtgaag tagagcataa cacccttttc gctgtatgct gctacggaag caaaagaagc
     8641 ctctccaaat ccgcggtccg tattgatagc aataagcgga ggaactttat cattttcgtg
     8701 agcagttgcg aagtctctga aagcctgacc tctttctaca ctcttaatag ttaaaccggt
     8761 tgtcttgttg ggattgttcc aatctgcggc cttaacgtaa acaccgcagc tgttgatgct
     8821 acccgactca aggcagagag caagctttac agaatccttg ctttcctcag caacgctgat
     8881 ggagaacttc ggagctgtaa ctgcgaatgc agaaacaagc attgcaactg taacaccgat
     8941 tactaaagaa acggaaagaa caactgacag gatttttgca atcttattca ttgtaatcac
     9001 caatttctcc ttcattgtag atttacacac atggtgcatg aatttatatt agacactgtc
     9061 gcccataggc gaataggaga caatgagcta acctaagtta atcaatagcc gaaattcgaa
     9121 taatgcgcgc cgttctttcc aagtgtcgtc atttcggcag taaggccgac ggtggtaacc
     9181 ttggagtcgg aaatacagct tgcgtaccat tcgatatcaa tgaattccga ggtcagcgga
     9241 tcaatttcac acttgataaa acaatcctta taaacaaaat caaatgattc gacgtttgat
     9301 tttgcaccgc ccacctcatt ttcgttcccg atcttctttt tagcctcatc atatgaatcg
     9361 cagccgagaa ctcttaccat ccgactgttt tcggtgggat tcttttcgtc cttaaatttg
     9421 acagtcagca tgtaatgacc atttttattt ttaaagcatt cgacatctgc ataacgggaa
     9481 atgtcggagc tctcaatcgc accgagcatt ttatcggctg aatcatcgct cttttgcttt
     9541 attatacttg aatagctttt gttatagctg ttttcttttt tcgttctctc ttcaatctct
     9601 ctgagccttg cggcgctaat caagggattc ttagagctga cctttatatc gtcaacgttg
     9661 tttgaaaagt tttcaagcaa tgtgtaagaa ggcctaaatc tttttgcttt ttcaatcgcc
     9721 ttgttataag tttcaactgc ttctgccgaa tcaacggttt caccgtcttc tgctttgaaa
     9781 cggtcgatta ttccgaccgt atatctgaga atttcaagtg catctatcga attaacgctt
     9841 ttatcggcat tgacatcggc gcgaaaaaac gcttccgaag gaagctcaag gttgccgaca
     9901 caatacttga gaaccgccaa agcgtcggcg gagttaaccg tcgaatctcc gtttacatct
     9961 ccgaggcgaa cactttctgc accgcacgcc acagggtacg acacaacagc ggcaagagaa
    10021 agaaaaactg atattgcttt ttcggtaaat tttcgcatct ctttcatcct tatcaacaaa
    10081 aatgcataaa cactcatgct atttttaaaa attatataac aaacatgata agatgtcaat
    10141 agccaattta agctaaaaaa agagcaactt aaaccggaaa aagagatact atttaaaagc
    10201 tgtttatccc tctgcccggg tagccgttga cattttgcga aatatactat ataatacaat
    10261 taacttaatt atatatcgga ggaatggaaa tgaagaaaat atcatctttg tgtgccgtaa
    10321 tccttgcagc ggttattatg atttcgtgcc ttatgatacc ggcctcggca gtcaactcca
    10381 aagtcgtgcg caaacctgac agcacaacct tctatcaggg cgttgattgg atgtactcaa
    10441 aaagcggcga aatcctcttg atgaagggcg gactcaacct ctcaggcaca tcaatttctt
    10501 acaataacaa aaccgtagat tataaaaaga gtaatttcgg tcccaatatg tatgcaaaaa
    10561 gcaattccgg cacatggcag gcaggcaaga atacaattcg tatttactgc gacagctttg
    10621 acggggtata tgctctttgc gaggtctatt ttgcttctgt taccaaaata agcattgtca
    10681 gaactcccaa aacgaagctt gtcgtcgggg tcgaatggaa ctacggcatc ggcaaggacg
    10741 ttgaaatgac taaatacgac ctcacaggta cgattatcag cgcaacatat gacaatgccg
    10801 ctacacaaac cgttgaagct ccgaatgcat gcttgaactg gtcgattccg gatgatacaa
    10861 acgaggtttt gccgacagac gatacacttt atataacctt ctgcggcaaa aaagctccgt
    10921 ttaatgtaac atttgttacc gaaacaagct tttcaaaggg cgatgtaagc ttggataaaa
    10981 aaatcaacgc ttatgatgcg ctgaatcttc ttcagtatgc aacgggttca attaccctca
    11041 gtccgacgca gataggtctc ggcgatatcg acggcaacaa aaaaataaat tctgccgacg
    11101 cgttgtatat tcttcaatat gttgtcggaa taaagaattc gctttgatta aaaggaggat
    11161 gaagaatgaa agcatttcag aaaattattt gtgcgatagt ttctctgtcg gtcgttgctg
    11221 cgacaatgac atgtatggct acctcatcat cggcagttgc aaacactccg ggcgatgtaa
    11281 acagtgacgg ctcggtaaac tcttttgatg cgctttcggt cgttagatac tgcacgggtg
    11341 ccgatacact caccaaggaa gaannnnnnn nnncagattt ccggctcaga cagatgcggg
    11401 acgagttcat cttttcggca atttctacta tgacaaagca accggcaaag taacgaatac
    11461 ggacggctcc ggtctcctcg gcttttcgta tgatgcaaag gacggagcat tttacgcctc
    11521 aacatatgcc tggcagagga cgttcggcta tacatatctt tatgacgtag ccgcaccgct
    11581 gattttatgt aactttgata caagccgtat cttcttcgaa tacaacgata tggaatggat
    11641 ggtacagctt tggaatggtc agtacggcgc aactttcaac ggctgcgagg ttggtcttta
    11701 ctatcgtgat tttgacgatg acgagcttgt tgatgccaac ggcagaaagt tctataagtg
    11761 cgccgatgat gaaatgctta tgcaaatgga gcttacaatg tataagaacg atactatgtt
    11821 cttccatcgc agcaagcagt attcatggtg gctcacaggc tttaagctcg gcactctcga
    11881 ttcgctcggc tactccgcgg aatccactca ggttcttcgt ttaagtgcaa caatttattt
    11941 taccgaagag gaaatgatgg acgcctttat cggcggactt gaaaaatgca cagaaattga
    12001 gcacaacgca tcaaagaaaa aacgtcagat taaatttgca aacggcaacg gctatgttgt
    12061 caacagagcc cagaaatccg taacattctc atgggtttaa tcgaaaataa caaaaaacct
    12121 accgcagttc aagctacggt aggtttttct atgtcatacc atatataaca tactaagaaa
    12181 cttattgctt aatctttcgg aaaatgggct ttgatagccg caaaagattc gtcgctgatg
    12241 tcatgctcta tcttgcatgc gtcctcggcg gcaattttcg ggtcaactcc cagcttcaca
    12301 aggaaatccg tcagaacggt atggcgctca tagattttct ttgcaacata aagtccgtcg
    12361 gcagttaaag tgatgtagcc gtctttatcg acaacaatat agtcgccgct tttgagcaga
    12421 ccgacagcac ggcatacgct tggcttggaa aagcccatat actcggcaac atcaagcgac
    12481 ctgacggcgc tgcttttctt tgaaagaata tatatagttt cgagatacat ctctccggat
    12541 tcctgtaaat gcataataaa gcctccaaaa ttacactttt aatattatat cacaacaatc
    12601 aaataaattc aagctcagtt atggttatct ttcttattat gaactcacaa aaataaatag
    12661 tattttagtt agacgcggct aactgtttgt tcatactgac aaatatcgtc gcaaaaacaa
    12721 aaaaattcgt tttttatatt gagatttctg ctttttggcg ataatatatt tctagttagc
    12781 gagagctaac cttatttaaa gccttcggtt agtctaaact aaccggcgaa agtgagggat
    12841 tagctatgat gcctttggca cttgcagatg tcggtgaagt gaacaccatt aaaaaaatcg
    12901 gcggaagtcc ggaagtcaag aaacatcttg aaaatctcgg gtttgttgtc ggcggaaacg
    12961 tgatggtcat taacacgctc ggcggtaatg ttatcgtcaa cgtgaaagaa gccagagtcg
    13021 ctataagcga agaaatggca agaaaaataa tgatctgatt cgtaatcgaa taccaaaaag
    13081 gtatttcaaa taatctgtac aatattaagg aggatgagac agatgaaaac tttaaaggaa
    13141 gcaaaggttg gctctactgt caaggttgtc aagctccacg gtgagggcgc agttaagcgc
    13201 agaatcatgg acatgggcat cacaagaggc gttgaggttt atatccgtaa ggttgctccg
    13261 ctcggagatc ctgttgaagt taacgtccgc ggttatgagc tttcgctcag aaaagccgac
    13321 gctgaaatga ttgaggnnnn nnnnnnagtg aattggctta actgacacat acggggattg
    13381 acccctattt ttttgaaatt acgattagca taagctaatt gaaagagagg aatttggtat
    13441 gaagttacgt attgcccttg cgggtaaccc aaactgcggt aaaaccacgt tgttcaatgc
    13501 gctgaccggt tcaaatcagt atgttggtaa ctggcccggc gttacggttg aaaaaaagga
    13561 aggtaagctc aaaaagcatg acggcgttat catcaccgac cttccgggta tttattcact
    13621 ttctccgtac accttggagg aggttgttgc aagaaattat cttatcggtg aacgccccga
    13681 tgcaattctt aatatcatcg acggtacaaa ccttgagaga aacctttatc ttacaaccca
    13741 gctcacagag ctcggaatac cggttgtagt cgcaatcaac atgatcgatg tcgttaaaaa
    13801 gaacggcgat aagatcaaca ttcaggagct ttcacgtcag ctcggctgca aggtggttga
    13861 gatttccgct cttaaaggaa caggaattca cgaagcggcg gaagcggcta tcgacgctgc
    13921 aaagaacggc aagacagttc ctatgcacac cttctcaggc tgtgtcgagc atgccatcgc
    13981 tcatatcgag gaagccgccg ttcacaatat gccagaggag cagcagagat ggtacgccat
    14041 taagattttt gagcgagacg acaaggttct cggaaatctt aatatagaca aggctgttat
    14101 ggatcacatt gagtccgaca ttaaagcggc tgagaaagaa atggacgacg acgcagagtc
    14161 catcataaca aacgagagat atatctacat atcctcaatc atcaaagctt gctacaagaa
    14221 aaagaatgcc ggaaagcttt caacctccga taagattgac cgtgttgtta caaaccgttg
    14281 gcttggactt ccgatcttcg ccgttatcat gttccttgtt tattggatcg caatggtcgg
    14341 tgtcggcgct cctgcaacgg actggatgaa cgacggtgtg ttcggcgatg gctggcacct
    14401 cttcggaatc ggctcatccg cttatagcga aaaggccgat aattatacaa atgcaacgga
    14461 agctgcacag gcatttgttg attttgacac ggaagcagaa gattttgatg cagacaagac
    14521 tgttgaagaa ctcaaaaact tcaagaccga caagcagtcg gcggttatcg gcgttgaaga
    14581 tgaagagaca ctcgctatca acgacatgac agtttactac agcgagattc ccgcaaatgc
    14641 cgataaagat tcaacaatcg gtatgactta tcttgaggct gttgattacc tcagcaaaaa
    14701 cggctttgac gagcccgatc cggctgacta cggcgtatgg gttccgggta tcccgacact
    14761 catcggaaac gcttttgaaa acagcaatgt tgcagattgg cttcagggac ttgtccttga
    14821 cggtatcgta gccggtgtcg gtgcggttct cggcttcgtt cctcagatgc ttgttctttt
    14881 cctccttctt gcattccttg aaggctgcgg ctatatgtcg cgtatcgcct tcgtccttga
    14941 ccgtatcttc agaaagttcg gtctttcggg taaatccttc atcccgatgc ttatcggcgt
    15001 aggctgcggt gttccgggta tcatggcttc aagaaccatc gaaaatgaac gtgaccgccg
    15061 tatgacaatc atgacaacaa catttattcc ctgctcggct aaggttccgt tcatcggaat
    15121 gattgcaggt gcactcttcg gaggctcagc tcttgtttcg acttcggctt acttcatcgg
    15181 tatgctcgca atcatctgct ccggtatcat gcttaaaaag acaaagatct tctccggcga
    15241 tcctgctccg ttcgttatgg agcttcccgc ttaccactgg ccgacagttt caaacgttct
    15301 ccgttcgaca tgggagcgtg gctggtcatt catcaagaag gcaggtacgg ttatccttct
    15361 ttcaacaatc tttgtttggt tcacaactta cttcggtgtt gttgacggag cattcagaat
    15421 gctttccgag gacgaaattg attcctccat ccttgcaaag atcggcagcg taatcgccgt
    15481 aatattcaga cctctcggct ggggcaactg gcaggcagcc gttgcatcaa tcacaggtct
    15541 tgttgcaaag gagaacatcg tcggtacgct tggtatcctt tacggcggcg gagacggaac
    15601 ggtttacagc aacattgcag cagccttcac gggtatcacc gcttattcat tcctcgtatt
    15661 caaccttctt tgcgctccct gcttcgctgc aatcggtgct atcaagcgtg agatgaacaa
    15721 cgctaaatgg acatggttcg caatcgctta tcagtgcggt tttgcatact gcatcgcact
    15781 catggtaact cagttcggca acatctttgt cggcaaggca aacgttatcg gcatcatcgt
    15841 tgcggccgct atccttatcg gtatgatcta catgctcttc ttcaagaagt acaccgaggc
    15901 tacaaagctt actcagaacg taaaggttaa aaaataaatc taaagaaaaa acgcacaatt
    15961 gaggtgtgcg aatgcaaaat atacagaaaa cgatttatgc aaaacgtgca ccgcaatttg
    16021 tgcaatattc cataattcaa tgtgaaagga actgatatta tgatttcatg gatttcacaa
    16081 aaccttgcaa ccattattat atgccttgtg cttgcgctta tcgtcacctt gataattgtg
    16141 ggcctggtga gggataagaa aaaaggcaaa tcctcctgcg gttgcaacgg tgcgaactgt
    16201 gcaatgagag gttcctgcca taaataaata gattttagat gttagatggc agacatcaga
    16261 ttgcttttga attaaattgc agatttgaat tttgcctgtc ttatatcttt aatctataat
    16321 gcaaattgcg ctttcgaaac gattcgtcta ag
//
LOCUS       DAWBSW010000022        15641 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_822_length_15641_cov_2.656615, whole genome shotgun sequence.
ACCESSION   DAWBSW010000022 DAWBSW010000000
VERSION     DAWBSW010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15641)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_822_length_15641_cov_2.656615"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7843..7852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9247..9256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4415 a         3546 c         3291 g         4369 t
ORIGIN      
        1 gagcactgtt gaaatacgat ttgcccgaat ttataaaccg tgcggtatgg agatcgtcgc
       61 tgtgaccctc gtcctcaaat tttccgtttt ccgcatggtt caccttgaat tcgatgccga
      121 gctgaacgcc gagagtcaca agatgctcaa catcgacctt gtctattttt cctgtgtgtg
      181 acggagaaat attcaatatc agcgagccgt tgtttccaac ggacttgaaa taaacctcaa
      241 gcgccttaga aagcacctta acgtcaaagt tttccgtctc atggaaaaac cagcccggtc
      301 tgatcgaaat atcggtaatc gacgggaacc atacaagctc ctttgctctt ttaatttttc
      361 ttctgctgcc gagatctggc tcaacaagct tttttgacgg ggcattgttt attaaatcct
      421 tcggcacaac gctccattca cttgttctgt tcactccgac atggttgccg caccagcgga
      481 tatcggggcc gcagttgcat atcatcgcat tcggctgata tttgcgaatc gtatcgtagt
      541 atctctgcca atcataattt tgctgaccgc tgttgtcacg ctcaaaccat acgcagaaaa
      601 tttcgccgta attcgtgcaa agctcagtca actgatttac aaaaaagtcg ttgtattttt
      661 ccgtgccgaa tgtcgtctca tgcatgtcat agggggaaag atagattccg aattttattc
      721 cttcggcggc acattcatcg gcaagctccc tgacgagatc accctcgcct tctctccaag
      781 gtgaattctt aacactgtgt tccgtatatt tgctcggcca aagacagaat ccgtcggaat
      841 acttgcatgt gagcactatt cctctcattt tcgccgcctt gaccgtactt acccattgct
      901 ttgcggaaaa atcggcaggc ttgaaatatt tttcgggttc acggcctgtt ccccattccg
      961 tattacagaa tgtgttcatt ccgaaatgga taaacgccat gaattcagta ttcataaaat
     1021 caatctgtgc ctttgacggc acaaccctgt ttgctctttc aatataatcc gacataataa
     1081 tacgttccaa tctaaaaact aaatatcttg tttttattga atttcataaa ttttacctcc
     1141 aaaatgatat gttttggagg ttgaaaatct acagtctttt gcaaccgcaa aatcatccgt
     1201 tgtttttcat tttttctttt atctgagttg tggaaatgcc ttgtgtgtac ggcaaatact
     1261 ctatgcttac tccgattttt gcaaattgct gttcggtttt gttgtatctc tccgtaccct
     1321 tccagtcatc tcccgaaaac aaaacatcaa atttatattt gctttggcaa aacattttat
     1381 cctctgtctc ctcgggattt attacaacga cctcatcaac gcatttaagc gccgccaaaa
     1441 ttctcactct gtcctcaagc ggataaacag gctccttatg ctttatattt ctcacataat
     1501 cgtcgtcgca gacagcgaca atgagatgag aacacatagt cttgcatttt tcaagcaaat
     1561 tcaaatgacc gacatgaaaa acatcaaaaa cacctgttgt aaaacctacg ttatacatac
     1621 tcatgccagc tccttttcag taaattcatt tattttttca aaaatctcct caggaaaaac
     1681 gacggtatct aacgggaaat gagaatgact taccatgggt gtcatataat tatttccgta
     1741 gtacagcttc aggtatgaat cataatcctt gggaatttta aaagttcttg tttcaaaaat
     1801 atgctcgtcc aattcttcaa taaatcttct gggaattacc ctgtgatcgg tgtgcatgca
     1861 aatattgaac agcctgtcat tttttcttcc caacaaacag attataaatc ttagtaacga
     1921 aatcaattta atccttatga actcaggtaa attctcggat atatcataaa ttcgtgtgtt
     1981 tggaaaatat atgttatgtc tttttttgta tctgttaaga tacttctcat gtatcataaa
     2041 tatacagcca agcagttttt tctggatttc taaaagcttg tcatttcccg cgcagtcgca
     2101 aaaaacaaaa atatctatcc aaagaccttt attgatatta agctcgtcag ttgcatactc
     2161 atcaacatat gtaccatcca gccttattct cggcaggaag tgaattgttt cgtgatcggt
     2221 cgaataatcg tcaaaaaata atccgtcaat acggttgttg tgtttcttaa aatacttctt
     2281 cagctttttg aaatctctgt tgctcattaa aacatctata tcatcatccc atgggataaa
     2341 tccgttgtgt cttattgctc ccaacagtgt tccgcagccc aaaatcaagc ttgctccgat
     2401 tttttcacaa atatcattta atctgcaaag catataatac tgtgtatgtt gaagatcttc
     2461 aagggtaaaa tctttatttt gcatttcaaa caccaccaaa tacgatatgt ctctttcgga
     2521 ttattttatc aattatttat ataaaagtca acatcaacct aacgttgttt tcttttgaat
     2581 caatgaaaaa aatgattgac tgttttcgat taaattagta taattatatt atctttataa
     2641 tggaggcagt atgtaatgaa aaagtttctc aaaatcatca tctcttccct gcttgtttcc
     2701 gcacttatga taggtgtagg aacggtagct ttcgcagagg aggacacatc aaacaagctt
     2761 acggttattt cttcaaatgt tgcgggactt ccgattccgt caaagtttga taaggacggc
     2821 aaggttgttc ccaaaactca gaaaatcatg ggcgaaatgc tcaacaacag cggcattgac
     2881 attatctgtg ttcaggaaga tttccagtat catgcgatcc tcgcaaagca gatgacaaac
     2941 tatccctaca agacatatac aagcggtggc gttcctgtcg gtgatggctt gaacattttc
     3001 tccaagtatc ccatctacaa catcgagcgt gttgaatggg aggcttacaa cggaattctt
     3061 gacgctgcaa acgatggcct tacccccaag ggctttctta aatgcacggt tgattttaac
     3121 ggtgttcttg ttgatgtgta tgacactcac cttgacgcta acggatctct cgccgactgt
     3181 gcggcgaaga aggctcagct tgagcagctt aaggcttaca taaacgaaaa ttcaaaggac
     3241 atgccggtta ttatcaccgg tgacatgaat attgctatgc actgtgacat caatgcggaa
     3301 ttctatcccg taatgattga aaacggcgga ttcaaggacg catggtcaga gtattgcaac
     3361 gacggcgttt atttcacaag ccgactctcg cccgaggtta atgcagaata cgaggcaaga
     3421 ttcggcggaa actattgggg tcgctgggat tccgttgagc gtgttgtcta tcgcagcagc
     3481 agtaacgttg agcttacgcc gacggatttc cgctatgagg actacaataa ccgtgacggt
     3541 cacggtgtag ttacggatca cagcgttatg atatgcgaaa tggaagttgc aacgacagat
     3601 tacgttgcac cggcaattga tcttaacaag gaaaaaagag aattattcgt tcataagctc
     3661 gttcgcactg taaagctcac gtcaagatgc atttacagaa ttcttaccga tcttatcgca
     3721 aaaatcaagt caggcgaaat cacaataaag taaggtgctt atataatgaa aaaaacgttt
     3781 aaaataatac tctcgacctt gcttgcgctt gttttgctga tttccgtcgg cacggtcgca
     3841 tttgccgagg acgaagatgt taatataacg gaagacaagc tttccgttat gtctgctaat
     3901 gttgcaggac ttccgatccc ctccaagttt gacaaggacg gcaaggttgt tccaaaaact
     3961 cagaaaatcc tcggtcagct tctcaacacc agcggtgtgg acattatctg tgttcaggag
     4021 gatttccagt atcatgcaat tctcgccgct caaatgaaga attaccccta cacaacctac
     4081 acaagcggcg gtgttcccgt aggcgacggc atgaatattt actcgaagta cccgatttac
     4141 aacgtagagc gtgttgcatg ggaagttttt aacggcatcc tcgacgctgc aaacgacggt
     4201 cttacaccca agggctttat gaaatgcacg gttgacttca acggaatcct cattgacctt
     4261 tatgacgttc attccgatgc gaacggctct ccggatgatg tcaaggcgaa acacgcgcaa
     4321 aatgaacagc tcgccgccta cattgacaaa aactctgcgg accgtcctgt tataatcaca
     4381 ggcgatatga atgtttacac tcattccgag caggatgtag gtatctatga aatctatgtt
     4441 tcccgtgagg gattcgatga cggctggaca atgttctgcc atgacggaat ttattttgag
     4501 cataatgtaa cctggcagga acgtcaggag cttgaacagc gctacggcgg aggtccgtgg
     4561 ggtcgttggg attccgccga aagacttctt tatcgcggaa attcaaacgt cggatttgag
     4621 gttacggatt tcagatatga cgactacaac gagcttgcag gacaacccgt ttctgaccac
     4681 aacatgatgg tttgcgagct caaggttaca tcgacagact atgtccgtcc cgagattgaa
     4741 ctcaatgttg aaaaaagaag acctcttttc gaaagaattt ttcacggcac aaaaatggta
     4801 ttcaggtgtc tcagactcat attcaccgac cttattgcaa aaatcaaatc gggtgaaata
     4861 acattcccga ccaaataaag gagttcacta tgagaccgat tacaactttt gtcagacata
     4921 attcaatcaa agcaattctc gccgcagaga agggattaaa agccgtatca aacggcaaaa
     4981 tcggcaaaaa tttatttgat attgagccga aatttatcgg tacggatgtc agaattatca
     5041 aggaaacgga cgattcaaca ttcatagcca aatgtgacac aaacggcaga atcgtcaaca
     5101 agccttttaa aatccttgta acgaccgacc ttcacctttg cgatgataaa gagctcataa
     5161 acaagtctct ttttatgctt gcaaagcacg ttgaagcaga aaagcctgat ctcatccttt
     5221 tcaccggcga tgttgttctg accgaccatc agcaggttga ctgtattcaa ttcggcaaat
     5281 ttatggaaaa gatgggcatt tactgggctt acgttttcgg caatcacgag gcaagggccg
     5341 agaaggaata tcacaaatat ttcatgctca aaaatctcat cagcttcccg cactgccttt
     5401 caaaattcgg agacccgtcg cttttcggtt acggcaattt tttcatcaat attctttcct
     5461 cgccaacctc gattcagcag agcattgtaa tgcttgattc gggcaggaat acaagcccga
     5521 cacacaatat cgaaaacggt gcgcctgccg agcttaaatg ctacgacttt ttgaaaaaag
     5581 accagattga ctggtacagg aaaacaatac ttgaccttga aaacgaatac ggtaaatttg
     5641 agtccataat ggcaatgcac attgctcttt gcgaatacga aaaagtaatg cagcttgatg
     5701 aaaacggtaa ttatattcct acaggcaagg ctgaaattct ttacggcgaa atgcacgaat
     5761 cgatcggatg ttcggaattt aacagcggaa tgtttgacgt tatcaaggag cttggcacca
     5821 cccaagctgt tattgtcggt cacgaccata tcaacgattt ttgcgccaaa tacgacggta
     5881 tttatctcgt ttacgctcag tgtgacggct acaacaccta cactatgggt tcaaacttcg
     5941 gctgggacga aaaggactgg atgcagggcg tcacaatgat cgacattgac aaggaaggca
     6001 aaattgtttt cagacagcgc ttcaacagag attacttata aggagatttt taatgacccc
     6061 ctatatactc aaaaaacatt taacacgcag aataactttt ttaaataaaa atgatccgca
     6121 agtccctgcc gctcttttca gtgcagtgag caagactccc gatctgtcga aatttcccta
     6181 tcccctccct tcagccaaaa gtgaatacac ctgctcggct gagggcgata attcggtcat
     6241 gtggtacggc ggcaaaaccg gaatcacacg ctatgacccg aacggtgaga gcatcgagga
     6301 taaaatcatg tatttcagcg ctcaccgtca ccttcctcat catgccgttg acgcaatgct
     6361 tgccgacgga atgaaccttt gggcactttg cagagataag gttgctcaca ttgagatgat
     6421 tatgctcacg gcggaggaaa aagcggatat tcttcttgac gagaccaata attatgttat
     6481 gcgtcgcgga atggtaagcc agcgtggtct caaggagtgc agagattatg aatcacgtta
     6541 tccttatgct gcctgcgata atgacggact tttcacctca ggctacgcta tcggcgaaat
     6601 gttccgttac gcaacgctca aagagaagct cggcaaggat catccgagag ttcgggatgc
     6661 aaggaaaaat gcaacaaagg cttgcgaggc ttgcctgctt ttgatgtata ttcacggcag
     6721 acccgaggga tttatctcgc gctcatatca cgtcaccggc gagccggtac ccgatgatgg
     6781 tattttctat aagagagagg gcaaaacagc tcattgcatt tttacaacag aggcaaagag
     6841 cagaggcaga gtcggtgaag aagttccctg cgattatccc attccggaaa gacttgccgc
     6901 tctttaccgt gacctcggct atacagacga cgatatttat tacaaagccg atacctccag
     6961 cgatgaggtt acgggtcatt ttctccagat gaaaatcgct catgattttc tcgcagagga
     7021 cgaccccgag cttgacgagc tcatcaagga cgcatgcaca agaacaacga agcacatcat
     7081 tgaccacgga ttcgaattct gcgaatcctc aggcaagccg accacatggg caaaatggag
     7141 caaacgttat tttgacaacg atcctatagg ctatgttgat gctccgctca attcctccga
     7201 aatgcttgtt tatctcaaga ttacaatgta cataacaggt gaaaaaggga tttggcagga
     7261 gacctatgac aagctcattt ccgagggcta tgccgacctc ggcgcaaagc actatgaccg
     7321 tttctatcag ggtgcaatga gagaaaaagt aaaccccgaa gaggatctca tgtacggcga
     7381 caatatgctt gcactgatga catactggat gctgtgcacg ctcgaaacgg acgaggagct
     7441 tctcgcaaaa taccgtgccg cattcaaatc ttggagcgga cttattctgc gtgagcacac
     7501 tccgggctac gactttatgt acaagctcgg cgttcccgat gcggaaattg atattgaaaa
     7561 ggatgcaaaa tggttcaccc atttcgaggc taaccgtttg attgcttcaa tcaactccga
     7621 gcgtcacgac gttgcggtta agctcggcag aaattacgac gaaaagagag agtttgaaat
     7681 ctcggctctg ctcatgccga atgaaagatt catttcaaaa tatgacagaa acccctatga
     7741 tcttttcaag ggtgaaaata aaggaacgtg catcgaaagc tgctacattt acacctatgc
     7801 ctattggata ggcagatact acggatttat tgacgaggag ggnnnnnnnn nnaatattaa
     7861 ccgaattcag cattgaagcc gtcaaggacg ctttgcagaa gctcgagtct ttcccaaatc
     7921 tcaaggtcaa cggtcttaac gcatatcagc tcaccgagct ttcagcgatt gatcccgagc
     7981 tttttgcgaa aatttccgac attatcaagg ctgaccgctg gtttccctat gccggcgtat
     8041 ggacaagcac cgacgagctt tcggaaattg cgcttgtcaa gagctgtctt tattccgtaa
     8101 gatatttcct cgataatttc ggcaagaaat accgtgtttt tcacggcagc aagctttaca
     8161 acaatatgct gccgcaaatc gtttattctt cgctttttga tgcagtcgtg ctcgaaagtg
     8221 agaccgaaag caagtggctt cacggcgcag acgatttcag aacactcgtt atgactgccg
     8281 agaccgttga cgttaacgac cttgacgacg acggcatttc aaaaaataat tttatatcat
     8341 atgaggattt ggcggataat ttctttgaag cccatcttga tttgaaaacc gtttttcttc
     8401 ctgccggcag cataaaaccc gatggaattg aaaaagctct cattgatgcg gaaaaatttg
     8461 ccgcaatcaa cagtgaggat aaaactgccg agattaaggc ggcttggctt gcctactttg
     8521 acggcgacct tgaagccgca agagagattg ccgacggtat tacgggcggc tcagccccgg
     8581 atgaaaacga cctcaagctc agtgatgccg gcattactgt taccgaaatc aagctcgctg
     8641 aggacggcag cggagataca gttatccgtg ttgccgaaac aaacggcaaa gaacactcgg
     8701 catacatcat gtgtgaccgt ctcgacgcag gcttccgatt tgagatcatg ccttacgaaa
     8761 tgccgacctt ccgtattccg aagaacacgg acggatattc aaaagaaatc tacatctgcg
     8821 aataaaaagc taaggattgt cgctcaattg cggcgatcct ttttattgca atatacctgc
     8881 tttgtgatat aattatatga aaactaactc ggaggggtta tagatgtcca aaaaaattac
     8941 gcttcacggc gaatttgtct acgttgcggc aatacttctg ctttcgcttg ccgttgcaat
     9001 gctcagcgca gcaaatttcg gcgtttcaat gatcgttgcg cctgcttaca taatcagcca
     9061 aaagctcggt gttatcacct tcggtcaggg cgaatacctt gttcaaagcc tgctttttat
     9121 tgctttttgt atactgatga aaaaggtcaa gcttgtttat ttcagctcgt tcgcaacctg
     9181 tcttatctac ggcgccgtgc ttgacctttg gcgtaaattc gttccgcttt tcaatcccaa
     9241 tatcacnnnn nnnnnnatat cactgctccc ggttcaatgg gcttgatcgt gcgtattctg
     9301 cttttcatcg gcggaatggt tttaacttcg ttctcggtta tgctgtttta caaaacgtat
     9361 ttatatcctc aggtctatga cttctttgtt aagggtatca gcgcacggtt taacaaggac
     9421 agaacaaaat ttaagattgc atttgatttt tcctgccttg ccgtttcatg cgtactcact
     9481 ctcgttttgt tccgcaaatt tgtcggaatc ggcgtcggaa cgcttgttct tacggcactc
     9541 aacggcttta ttataggttg gtttgacaag ctgtttgaca aatgcgttga tctcaagcca
     9601 atgttcccga aatttgccgc gaaatttgat ttataaatcc gtaaaaaaga attaacaaat
     9661 aattcacttg aattatcggg caaaatgttg tatgcttata tcaaaggaaa atgcttttgc
     9721 ttcagtcgtt ttcctaaatt acggaggtaa acaatcatga aaaaaggttt atgtatagtt
     9781 ctttcggctc ttatgatagc cttctcgttc gcatcgtgcg gcaagaagga cgtcagcgac
     9841 aagccgacaa aaattgacga aaacggttca gcttatgtcg aggttaccga caaggacggc
     9901 aatgaggtaa catccatcct ttccgataag gacaaagcaa aggctgataa aaatgcagct
     9961 aaggacaata aaaacaataa caatacaacg acagtagatg cctctgagct tgcctcaaaa
    10021 gcagaaggag caatgtcggg cttcacaaat gttgatgaaa aggatatcca gtccgataag
    10081 aaagacctta ttaaagacgg tacatccact aaaaaaacat ctctgcgtga tgacgttatc
    10141 atcaaaacat caaaaaccgg aaaattcact ttgaaagcca agatcaaagc agcatccggc
    10201 gagaataccc ccgtaacatg cgttacaaac ggcaaaaaat ttgcttatga catgacaatg
    10261 aacggttcgc aagtcagaat aataatggac ggtaacaacc tttatttatt gttacctgct
    10321 attaaatggt atgttaaaat gtcaaccgat gacattggcc ttgaaagcat ggacgatatg
    10381 atgtccaacc ttgcaagtac caaagataaa tatgtcggct caacaaaggt aaccgttaac
    10441 ggcactgaat acacttgtga ggaatataaa aacagtgacg gaaggataac caagtattat
    10501 tttgataaaa acaataattg gaagcgtatt gaaggaatta acggtgacga agttaccatt
    10561 atggaaattg actcattctc aggaaaagtt gatgaaagtg tattctcact caaaggctac
    10621 acggatatga ccgcccttgt gatgggacag gcaaatatca ctacaaccac taaaaaaagc
    10681 taatgaaaaa tgtacttata acaggctcgg caagaggaat cggtgcacag acggcgaagc
    10741 tttttgccgc agagggctgc actgttatcg taaattataa tacaagtgaa aaagaagccg
    10801 ctgagacttg tcgcgagata aggctcagcg gtggcaattc tgtcatgatt caggcggacg
    10861 tttccaaaag gagcgaggcc aagcgccttt ttgccgaagc aaaaaaagaa ttcggccctg
    10921 ttgatgtgct cgtgaataac gcaggcgttg cacaaatcaa gctgttcaca gacatcacgg
    10981 atgatgacta cgaccgtatg tttgattgca atgtgagaag cgttttcaac tgctgtcagc
    11041 ttgcacttcc cgatatgatt cacaagaagc agggcagaat tataaatgta tcctccatgt
    11101 ggggaatcac aggagcctca tgtgaggttc attactcggc atcaaaagca gccgtaatag
    11161 gattgaccaa ggcattggca agggaggttg ccccatccgg aattaccgta aatgccgttg
    11221 ctcccggagt tattgacaca ccgatgaaca gcggctttga caaagagaca ttagaagccc
    11281 tcaaagaaga aaccccggtc ggcaggatag gcacacccga ggacgtcgca agagcgatac
    11341 ttttcctcgc agacgaaaaa tcctctttta ttacgggtca aacgtttggc atcgacggcg
    11401 cttttatctg aattataaaa aggctgattt ttttcacaaa ttgtatttat acaatttgta
    11461 gaaaaagctt cttgatttta tgataaatat gtgttagaat atcctatacg ctaaagtgat
    11521 tactgaaatg gaggaaatcc ttatggcagt ttgccctaaa tgcggaagaa aacttaaact
    11581 tacagatatc aagcccacat gtcccggttg cggagttaat cttctttatt acaagattga
    11641 agaaaggctt gaggttgacg ctatcaatgc cgagcttgag cacgcaaaaa ctcaaaagag
    11701 ggttgaccgt gccaaggacg caatgttcgg cacacccctt gcaatcgtaa gacttgtttt
    11761 tttggtcctg gttgtcggaa tgttctttgt tccccttgca acattccatg ctgtaggccc
    11821 ttattttgac aaaacaacga cgctcaatgc tcttgagatt tacaactcgg tttcatctct
    11881 tgactttgac ggaatgtttg ttctcgcagg ctcgcctgtt ctcaagacaa gcttcatgtt
    11941 ccttgcaatt tcagctgtgg caatcgttgt ttccgttctt tgtgcgctgc ttgagctgat
    12001 tttcagcttt ctttcaagct caccgcacgg tttttcaagg aacataaccc ttgcttctgt
    12061 cggcattgtt tccgcagtcg cttcacttgt tacatataaa aagttccttg caaatattga
    12121 atccgttttc aacggaatca tcgacggctc cgttaagatc ggtatttacc ttgtaattgc
    12181 cgcatttgtg ctttgcattg caatcaatat cataatcaaa gttaagcata aatccattaa
    12241 gtatacggac acatacgttg accgtgttcc ttatgagatc tttgtcgaaa agtttggcgt
    12301 taaaaagtac gacatggaat ctcttgatgc catcaaggct gacatggaac agtacaaggt
    12361 cgttgaagaa taaataaaaa atataatcgg tcataagtct tagtaattaa gctttatgac
    12421 cgatttttaa ctaataagac tgcaagccat aataatttac ggtttgcagt cttttgtttt
    12481 atttaagcat tgaaatagcg tcggtgatcg tcttttcgta ggcttccttg ccatacttat
    12541 cgacctcggc cttattcctt tcgccgtggg tcaagcagcc tgcgccgccg atacagccgc
    12601 cgatacaagc catgccctcg ataaagtttg cgtcaagcct gtccttgctc tttttgagca
    12661 atgcggcacg gcaagcttca attccgtcac aggcacacgg tttaagctca aagtccttta
    12721 tgtcctgctc cttgagagcc tgtgcaacgg catccgaaag accgccgctt cgtgcaaata
    12781 tccgtccgaa gtaggaagcg ttgtcaagca caccctcgct gagcgtcata atgtcgatat
    12841 ccttgctgtc aaagagtgcc tgaagctcct caaaagtcat cgcaacatca acatagggtg
    12901 caacggtctc cctctgaacc tccgcctttt tcgccgtgca cggtccgata aatataacct
    12961 tacagttagg ctcacgggat ttgatatact ttgaaaccgc tgccataggt gaaagattgt
    13021 gcgaaacaaa cggcaaaagc tgcggaaatg tcttttcaat ataagttaca aatgccggac
    13081 agcatgagct tgtcaaaaat cccttttcgg caagctcctt tgactcggaa tatgcaacca
    13141 tatccgcacc gagagccgcc tcgataaccg tgtggaaacc aagctcttta agtcccgtta
    13201 taacctgtcc aagctttgca tacttaaact ggcttgaaat tgacggcgca acgacggcat
    13261 aaaccttgta tttcttatta ttctcgctct tttttattat gtcaacaacg tcgagaatat
    13321 atgacttgtc cgttattgca ccgaacgggc actgataaac gcaggcgccg caggcaatgc
    13381 acttctcgtt gtcaattgcc gccgctttgt tctcgttcat cttgatagcc ttaatcttac
    13441 aggcattctc acagggacgc ttacggctga taattgccgt atagggacaa actcttgaac
    13501 aggcgccgca ttccttacac tttgttttat caatatgtgc aacgtggttc tgatcaaatg
    13561 tgattgcacc gaaacggcat gcgtccatac aacggtgtgc aagacagccg cggcatgcgt
    13621 ttgttacctc atagccaccc atcggacatt cgtcacaagc agtttcaata acctcaataa
    13681 cgttcgggtt tgacttgtca ccgcccatag cgattttcac acgctcgccg agaattgctc
    13741 gctctttata aacacagcaa cgcatagtcg gcgtgttgcc cggaacaatt gttttcggaa
    13801 tttcaagaag attgtccaac agtgtgtcat cccatgcgtg gcgggctact tcacggagaa
    13861 ccttatattt gagatactga accttggtgt caaatttata tagcattgtt ttcctcctta
    13921 gaaataactc acctttattc tcttgcccat ttgcttcaag cagctcgaaa gctcctcaac
    13981 aagattcatt ttaatattga aattaatcgg caaatccgga ttttgatgag caggattgac
    14041 tgctctgccg acataaaaat tgatatctgt tgcttcctca aacagcaacc ggcaaattct
    14101 tgatgctccg tcctgcttga tgctccactg atcgtaaagc tcattgctgc ttaaagcatc
    14161 cttggcgtac tcaataacct tgtttattgt aataacgccc tcagttacaa ggtcaacacc
    14221 ctcaatttcc gcaatcggcg gaacatcgct ctgctcaaaa ttgagcgttg ccctgacggg
    14281 cttgccaagg tatttcgccg caattgatga cgttgtaccg ccgcagacaa tgtgctttcc
    14341 ctcctttgag aagaaaaggc tcatcatgcg gtcgcagtcg tctctgttgc gaggcggacc
    14401 aaagagcaga ttcatcggct ccctctttct gacacgaaca acacacgccg tcgcatcgtc
    14461 gccgggatgg tagccgtact gcttgttgca ctcatcaata agcatcgttg aaagagtttt
    14521 tgccgtgtag ccaaggtcgg ctatcatttc catgaaactg ataatatcat ctctgcgcca
    14581 gccaaaatta taggcaatac ctattccggc gtggggacag ccgtcgctca tggcgacaaa
    14641 aatatcgttc tcctgcaaat caataacgga tttgtatatc tgcttgccgt ttatattcag
    14701 ctcatttttg ggatagtcaa aatttttgcc ctccctgagc aatatcacat gggggttgtc
    14761 gtactgaata agttcggctg tctcattgtt gatcaggtga ataatagtga aagttgaata
    14821 tgcaactcct ctgacagagc atatcggaag cgttgcggca atggtcgaaa cacattcctc
    14881 aagggaaagt ccctctgcca tcatcgttga aattatcttt gatgttagcg ttgaaaggat
    14941 gctcgcctta actccgctgc cgaggccgtc cgccagcacg ataacagtcg aattctcatt
    15001 ttgttcaact atatccacat ggtcgccgca gagctgttcg ccctcgtgat ttatactttt
    15061 ccagccgatg tcagcacaaa gattattcat cgctgatcga ctcctttaat ttagtaagcg
    15121 caatttttgt ctcagccgcc gtttcgccga gaagtgaagc gatctcctga acgattctca
    15181 tctgattatc aacaacacgg tcggcaatct caactgtctg gtggctgatt ttttcctttt
    15241 tctccttctg attttcttcc tcggtaacat cacgaagaat acaaaccagc aggtggtatt
    15301 ccttatcgta aaggattgat tcctcaacat attttccgta ctcggcaagg tatgttctct
    15361 tgtcatggat acttctgccg ctgttgagaa cgctgaggaa atcggtcgga tcaagaattc
    15421 tgacaacctg atcgccgaga acaaccgaag cgttacgaac gttaacaatt ttgagcgcag
    15481 cgtggttgat ctgctgaacc tcaagctttt cgttgagaac gataagtccg ttcggcgtat
    15541 tacgcacgat tgtgtcggag aagctctccg ccttgtcctt gaggtatggc agacacatgg
    15601 aaatctcagc cttgccctga agaattgcaa cagccttttc a
//
LOCUS       DAWBSW010000023        15519 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_832_length_15519_cov_2.393689, whole genome shotgun sequence.
ACCESSION   DAWBSW010000023 DAWBSW010000000
VERSION     DAWBSW010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 15519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15519
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_15519_cov_2.393689"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1523..1532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2912..2921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3641..3650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7015..7024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10563..10572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13490..13499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14163..14172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15107..15116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3970 a         3444 c         3684 g         4341 t
ORIGIN      
        1 aaaaacaggc gactgttacg ttgaactgaa gctcgttgac gagagcgaaa gcgaagattt
       61 ttcgattgaa catgaaaaag aaataattac ggttacggcg catcgtttgc gaggcttgat
      121 ttacggctac tctgtctttc tcagaaaatg cagggtgatt gacggtgagc ttgcactgac
      181 gaaagatata agcgaaaaat attcaccgta tatgcctata agaggtcatc agctcagcta
      241 tacggacatg aacaatacat atgagacgtg ggacgaaaag caatacgaaa gatatatccg
      301 tgatttgatg atgttcggaa cgaacacggt tgaggcgtgt ctcggcgacg atgaaaagca
      361 cacggatttg atgaaataca gctttgaaga aatcacaaaa attaaatcgc aaatttgcga
      421 aagacttgat ataaattttt caattttctg cgcttatgca aagagacttt ccgacgaaga
      481 aagcagcgat tatttctgcg gctgttgtca taatataccg aaatttaatt tttattttcc
      541 gccgggagga gatccgggtg atttgcaggc tgaagacttt tttgtgcgct gcaaaaaaat
      601 aaaaaaagat ctgcaaaagg aatttccgga cattgagctt tggccgtcgg ctcaggctcc
      661 gcatcagtat gccgattggg gccaacggtt tataaaagaa atggcaaaaa tgcccgagga
      721 aatcgacgga cttatttacg ggccgaatca tccgtttacg ctcgatgaaa tgcgccgctt
      781 tgttgatgtt aaatacccga taagatacta cccggatatt tgccacaatt tgcgctgtga
      841 aattcccgtt cactttgacc gtgacgattg gcactacgcc tatgccgcaa cgctcagccg
      901 tgaagcaatc aacccgagac cgagcgagta caggctgatt caccgcctga cgaagcaata
      961 tgtttgcggc agcgtttctt attccgaagg ggttaatgac gacgttaata aattcgtttt
     1021 cggcgcactt gattttgacc ccgatgccga cctccgtgaa attctcaggg attatgcccg
     1081 tgcgtttttc tacggcgagg actgcgaaaa aattgttgac gtgatttttg gaatggagca
     1141 gagctggaac ggtgaccccg ctgagaattg gtcggttgaa aatgtttaca aggcgcttat
     1201 tgaaatgaaa tcggataagc taatgaaaaa ctggagatat gttcttttcc ttttccgagc
     1261 gagctgtgac aaaattgttc gcgacagaag aatttttgag cttgatttga ttgatgacgc
     1321 aaggactcag ataagaaaag gcaatattga gcttgcaaaa gaaattcttt caacggattt
     1381 tgacgaagaa tataaagatt tgcgagcgga attattcccg cttgcagaga agctttttaa
     1441 tcttatcgga atgcagcttg atgttgagca tttcggcgga atgaacgtcg aaagaggttg
     1501 tgttcttgac acgatcgata tgnnnnnnnn nncagggttg aaaaggacga gtattatttc
     1561 tcgtttgctg agcacggctt tgaggtctgc ggcaagcaaa agggcgaagt ctatatgaat
     1621 ttccaagggg acgataatgc cgatgcaagg cttccgatgt gcattaccaa ggtttatgac
     1681 catttcaatt ttaactgtaa tgttgcgggg ctgacaggcg gcgattataa gctgcgagtg
     1741 acatataaat cacgcccgaa cgaaaaaata aatcatcata aaattacagt gaacggcaac
     1801 gttatttatg acgggccgca gttcggcggc agacgtgacg gggagtacga aaagaaattc
     1861 cttgccgacg gatatcaaag tattgtctat gacgtgccga aagatttttt gcaaaacggt
     1921 tgtgccgagc ttgaaattac agagccgctc gacggcttta tgataagcga atttcgcttt
     1981 gtgaaaaaga ggtgagccaa tgccgaaata cagtgttttg ctgacggttt acaacagtga
     2041 aaaatatctc gatgaatgct tcaattcgat tttgtgccag acctttaacg attttgaggt
     2101 ggttgttgtc gatgacgcct cgacggattc aagcggaaaa atatgtgata aatatgcccg
     2161 aaaagacagc agagttaagg ttattcacaa ggcaaacgaa ggagtaaacg ttgcccgtaa
     2221 aaccgctttt gaaaattcaa cgggagatta tattctgacc gttgattgcg atgacataat
     2281 tgatcatgat ttaattgaaa cggttgacgg aatttttgaa aaatattcct gcgatattgt
     2341 catgttcgat ctgtcgattt ttgataacaa aacgggcaag gttacaataa aaaagatggc
     2401 ggaaaaaagc tgcttttacg ataaaaaaaa taaaaaagaa ttgtatttga ttttgctgaa
     2461 tcgctgcatg aattcacttt gtacaaaatg ctgccgcaga gaaatttacg gcaggtcgtt
     2521 tgactttttg aaatatcttt cgatgtgtca cggcgaggat tggttccaat cggcaattca
     2581 ggtttacaaa atgaaaacag gctattatct tcagcgaccg atgtatcatt atcgaaacct
     2641 cggctcgagt ctttctcaca gctttgacct cgactcattc agactcaatt ccgattcgct
     2701 ttcggacgtg aaagcgatga ttattgatga cggatatttt gacgccgaag taaaagcgat
     2761 gtggaaggca tatgcaagga aggttgtcaa tacacttttg ctcggacttt caagttccga
     2821 tttgaacatg aaagaaaaaa tcggcacggt caaatcagtg gccgataccg atatttataa
     2881 aactgcgatt gatagcgatg ccgattacgg tnnnnnnnnn ngaatgacag ttcttaaatt
     2941 ccgcttgctt aaaaacaaaa tgtacagatt gctttttgcg ctgatgaaaa acagaaattt
     3001 atgatttttg caagctctta tcaatatgaa tcagcactgc gagagaagcg acaaaggtta
     3061 tgatatcggc tattgccatg aaccaaagca gtccgtcaag tccgaaccaa atcggaagca
     3121 gaagcacaag acctacgccg caaacaattt ctctgagcac tgagagcagg gttgctgttc
     3181 ctgccctgcc gagggattgc agaaaaatcg taacaccctt gttgacgcat gaaagaatcg
     3241 tagcgcaaag aattatgcga atagctttta ttgcgaactg cgtgtagtaa acgctctcgt
     3301 tgcctgcgcc gaatatgccg ataagctgcg tagggaaaag ctcaaaaata attgttgcaa
     3361 caagtcctgc ggttgcctct gcaagcatga gcaaacgcat aagctcacga actctgtcct
     3421 tgcgacctgc accgaggttg aacccgacga tcgggataca gcctgccgac atgccgattg
     3481 cgatcgacat gattatctga aaaaatttaa gaacaatgcc gatgacagcc gtcggtattt
     3541 gggcgtactc gttctgtccg aaaatcggat cgagtgcgcc gtattttttg cacatattga
     3601 gcaccgccgc cattgaaaag acgacggaaa tctgagcaag nnnnnnnnnn gatttgtcgg
     3661 gaacaaagct cgccctgcag agctttacgc tttttagttt aaaaagataa ataagtgaga
     3721 gaacagcgga gagaatttga ccgatcacgg ttgcgaccgc agcacccatc atgccccaat
     3781 gaaatttaaa aatgaatatc gggtcgagaa tacagttgct tattgcgccg acaagcaggg
     3841 tgatcatggc gaaacggggg ttgccgtccg aacggattat cggattaagc gcctgaccga
     3901 acatataaaa cggaacaccg agcgaaatcc aaaagaaata ttcctttgac agcctgaagg
     3961 tttcttcgtt gaccgtgccg ccgaaagcgg ttataattcg gtcggcaaag atcaagtaaa
     4021 ttaccattag aacaatgctt gaagcgacgg tcagaagaac cgaatttccg atgcttttgt
     4081 gcgctttttg aaagtccttt gcaccgagac gaatgctgac gaatgcgccg cagccgtcgc
     4141 cgatcattgt tgctattgca agggcgataa ccgtcagcgg aaatacgacc gagtttgccg
     4201 cattgccgta agagccgaga taatcggcgt ttgcaatgaa aatctgatcg acaatgttgt
     4261 agagcgcgcc gacaagcagg gagattatgc acggcaatga gaatttgcgc atgagcctgc
     4321 cgattcgctc cgtttccaaa aatgaattga cctttgtttc ttccataatg aatcctcctg
     4381 tcgctttaca acacaaacaa aaagcacacg cctaaagcgt gcgccgtaaa gccggagtaa
     4441 aacgatacac gccaagcctg caccttgtgt cgtatcagga cagggagtcc gtgtatcgtg
     4501 tcttcaagct gaaatattta ttattgcaaa ttgaattact tttaattcaa tcaacgatta
     4561 attattatac attgattttt tgaatttgtc aaagaaaata taaggataaa acagaaaaaa
     4621 gcttgcataa tttaaaaata tatggtatat ttataatcac cgaaagggag tagtcggcga
     4681 aaaccgttac attcagcgtc aattcggtca aaaagacccg cttttgtatt aaatgacgag
     4741 acttttattg ctattgcagt agaagtctct ttttttatgc cgattcttcc gaaaattttt
     4801 aaaaaggagt ttgtttatgg aatttttctc ttttctgtat caaaatgccg tagataattt
     4861 tgattacaga atgcttatca tgtgggcgat aggcggattg ctgatttttc ttgcaatcaa
     4921 aaagaatatg gagccgacgc tgcttttgcc gataggcttc ggcgttattc ttgttaacct
     4981 gccgctctcg ggagtggttg accgtgtggt aaacggcttg ccgcaggagg gtgcgctaag
     5041 tattctctac aagtcaggaa tcgcaaatga gctttttccg ctgatacttt ttatcggcat
     5101 aggggcaatg atcgatttcg gaccgattct tgcaaatccg aagctgatga tattcggtgc
     5161 ggcagcgcag ttcggaatat tttttaccct ttgcacatcg tcaatgtttt ttgacataaa
     5221 ggacgctgcg gcgatagcaa cgatcggagc ggcagacggc ccgaccgcaa tagtggttgc
     5281 tcaaatgctg gattcgaaat atctcggcgc aataatggtt gcggcatatt catatatggc
     5341 acttgtacct atcattcagc cgccggtaat aaagctgctt acaacggaaa aagagcgaaa
     5401 aatacgaatg aaatataagc cgagtcaggt ttcaaagctt acaaaaatac tgttcccgat
     5461 tgtgataaca ataattgcgg gagttgtttc gccaaactcc gttgcactcg taggatttct
     5521 catgttcgga aatcttatcc gtgaatgcgg tgtgcttgat tcgctgtctg aaacggcgca
     5581 aagagtgctt gcaaatcttg tcacgatttt tctcggaatt accattgcga ctcagatgca
     5641 gtttgacaaa tttcttcgga gggagacgct gcttattctc ggcctcggat ttatagcgtt
     5701 tatattcgat actgcaggcg gtgttatttt tgcaaaattt ttaaatcttt tcctcaagga
     5761 aaaaatcaat cctatgattg gtgccgccgg aatttcggct ttcccgatgt cgggccgtgt
     5821 tgttcacaaa atgggccttg ccgaggatcc tcaaaacttt ttgcttatgc agtcgatggg
     5881 cgttaatgtt tcgggtcaga ttgcctcggt aattgccggc ggactcatac ttaacttttt
     5941 tgcatgatga aaggagattt atttatgaat ttattaggtt ttaatccgta cgctttcata
     6001 gaaacgctcc catatatggg aaaaggtatg cttggaattt ttattgttat aggaattata
     6061 ataatttgtg caacaatttt gaataagctg acaggaaaga acaaatcaaa agacaataaa
     6121 taaaaacagc cgcaaagcaa gcatcacaat ttgctttgcg gctgaaattt tgtcttattc
     6181 aatactcatc gtcgcatagg cgttcaatga catatcggcg gtgttgccgc tgatgaattc
     6241 ataatagccc tgtgcaccga ccattgcgcc gttgtcgccg caatatttaa gctcgggggc
     6301 aaaaagctcc caaccgttct ttttgcaaag agcctccata tccctgcgga gaaccgagtt
     6361 ggccgaaacg ccgccggcaa gcacgatctt tttataaccg aggtcctttg ccgctttctc
     6421 cgtattttcg gtcaggcaac gcacaaccgc cttgatatac gatgcgccaa ggtcgggaac
     6481 gtttatctcc tcgcccttct gctttgcaat atttatggtg ttcaatgcgg ctgttttcag
     6541 tcccgaaaag ctgaaatcgt aaggtgcgcc gtcaactctc gggtgcggca ttttgtatgc
     6601 cgtctggtcg ccgtcctttg caacacggtc aaggttcaga ccgccggggt agggcagacc
     6661 cattgctctt gccgccttgt ccattgcttc gcctgccgca tcgtcatgcg tgtagccgac
     6721 gattttaaat ttcgtatagt ccttaacctc gacaatgtga ctgtgtccgc ccgaaacaac
     6781 gaggcagaga aattcaggct caagctcggg gtgggtgatg tagtttgcgg caatgtgtga
     6841 gcgcaggtga tgcacgggaa caagcggaat tccgctcgca agcgacaggc cctttgcgta
     6901 atttacaccg accaaaagtg cgccgataag tccgggcgca tatgtaacgg cgattgccga
     6961 aatgtcgtca agtttgcagc cggcatcact cagagccttg ttgacaacgc cggannnnnn
     7021 nnnnacgccg gaaacggctt cgacatggcg acgcgatgca atctcgggca caactccgcc
     7081 gtagataata tgttcctgca cctgagaagc tacaacgctt gacattacct ttctgccgtc
     7141 ctcaacgacg gcggccgcag tctcgtcaca cgaggattcg agggataata ttttcatttg
     7201 atatcacatt ctttcaatgt catgatgacc gcatcttcgg cgggatttgt atagaaattt
     7261 ttgcgctcgc ctgccgtttg gtagccgagc tttttatata gcgaaatcgc tttttcgttg
     7321 cttctgcgaa cctcaagcgt aatgaattca cacttcctgc cgaacgcatt ttcctgcgcc
     7381 gttttgagaa gcttttcgcc gatgccgagc cgcctgtaat cggggcgcac tgcgatattg
     7441 tcaatgtacg cctcgccgca aacctcgtga acgccgatgt agccgagaac atttttgccc
     7501 gactgagcga cgagaaaacg agcgttttca ttgtcaagct cctcggcgat ccctttttcc
     7561 gtccacggag cggagaagca gagtttttct atctcggcaa tgtcggaaac gtgctcggcg
     7621 gacatgggct tgatttcggc agtcagggcg tcgagaagcc ttgaaacgaa gctttcaatc
     7681 tgatccgcac aggcggagta aatgtcctcg tcaccgccgt aagggtcgga gatttcgggc
     7741 tcgggaacaa taaccttgaa tttcggattc agcgtcatta ttgcctgctt atgcgcctta
     7801 gtcatgcaga ccatgaggtc ggtttcgtca aaaatatatt ggttgatctg ccttgaacgg
     7861 tggtcggaaa tgtccgcacc gtgcattctt gccgcccaaa cggcaagcgg cgatgcactg
     7921 tcgcccgtaa atgcaccgag accgcagctt tggcagaata tgtttttaat ttttctgtcg
     7981 gcaatgagcc ttttgcaaat gccctctgcc attgcgctgc ggcaggtatt gcctgtgcaa
     8041 acaaaggtaa ttgtcatatt atcatcctat cagatagcgg ctgtcaagcg ttccgttaaa
     8101 gaattcgata acgcttttgg cagccctgtt gttcatttca cggaaatttt cttttgtaaa
     8161 aggagctgtg tgaggtgtta agacaacatt cggcaaaccg ataagagggg agtccgtgca
     8221 aggctcattg tcaaagacat caattgctgc acccgagagc ttgcccgatt tcaacgcatc
     8281 gacaagcgca tttgtatcaa caattccgct tcttgccgtg ttgattagaa ttgctccgtc
     8341 cttcattttt gaaaatattt ttttgcctat catgttgacg gtgctttcgt tgagcggaac
     8401 gtttattgaa acgtaatcgc tttttgcgag aagctcgtca agctcggcgt aatgtgcacc
     8461 gaattcatcc ttggggtggc ggcaatagta aagcacatcc ataccgaacg gaacggcacg
     8521 ctttgcgatt tccttgccga ttccgccgaa tccgataagg ccgagcgtgt gactttttgc
     8581 gcccggggtc atgattcgct tccattcgcc cttgttcatg ctctcgcttt gcaggtcgat
     8641 tcgtcttgca aaatagagga tataagccat aacgctctct gcaaccgagc cttcaaccgt
     8701 accgagagca cgggcaactg caacgccgtt ctttttgcag aaatcaatgt ctatcgaatc
     8761 gtagccgatt ccgcggcggg agacaatttt gagatttttg ctttcagcta ttgctttatt
     8821 acttactttt tcgggaccgc atataactgc gtcggcgtca agcacaagcg aacgtatata
     8881 ctccttgtcc gtgccgctgc ctatgtcctc tttgctgtga tcctcaacct caaagcctgc
     8941 atcctgcagc atttgcaatg ctgttttgtc atctgtcgag aaatttcttg ccgtaacaac
     9001 aaccttcatt ttgcattatc cttttgcgtc gtaccagctc ttgccgcaat gaacatctgc
     9061 ggtgagcttt accttcatct tacatgcatt ttccatctct tcacggatga ttttttgaac
     9121 ctgcgcctgc tcattctcgg gagcctcgac aataagttcg tcatggatct gcataataag
     9181 ctttgatttg agattttctt tttcaagcct gtcgtagaca cgaaccatcg caattttgat
     9241 tatatctgcg gcggttccct gaataggcat attccttgcg acacgctcgc cgaagttgcg
     9301 gatcatagca ttcgacgcgg caagctcggg cagatagcgg cggcgcttga acagagtctg
     9361 aacatagccg tcctccttcg ccttggcaat gacattcttc atgtagctgt caacgcccga
     9421 aaaatgatga agatagcctt tgatatattt gtcggcttcg gctctcgtta cgccgatgtc
     9481 gtgggcgagt gagaatgcgc cgatgccgta aactatgccg aaattgaccg ccttggcacg
     9541 gcttctcatc aacggagtta ccgcctcaac gggcatgttg aaaacctgcg aagccgttat
     9601 tgtgtggata tcgtcgccgt tgttgaaggc ggatatcatt gtttcgtcgt ttgcaatgtc
     9661 cgagagaaca cgaagctcaa tctgcgaata gtcggcgtca acaagtacac agccgtcctt
     9721 ggcgataaag aattttctca gctcgcgtcc gacgggagtt ctgacgggaa tgttttgaag
     9781 attaggttcg gtcgatgata ttcgacccgt ctttgtctcg gtttggttga gcgtcgaatg
     9841 aattctgccg tcaggcgcaa tgcatttctg caagccgtcg cagtaggtcg atttgagctt
     9901 tgcaagcgtt ctgtattcga gaatgagcga aactatcgga taatcgggtg caagaccttc
     9961 aagcattggt cgaataaccc gattttgttt ttttctttgc cggcagtccg agcttttcaa
    10021 aaaggattgt gccgagctgc tttggcgaat ttatattgaa ttcctcgcct gcaaggtcat
    10081 aaatctcgct gaccagggag ttgatttttt cttcaagcac cgctccgtat tgttgaatgc
    10141 tctcacggtc aacaagaaat cccgtaacct ccattgagga aagcaccttt gaaagcggca
    10201 tttcaatatc gtataggagc ggaagctggt cattttcctc gatttgcttg atgacctttt
    10261 cccatgcgcc ctttaaagcg gcggctttaa gagcggtctc attttcttcg cttatttcgg
    10321 gacagagtgc accgtattcc tgcgccattc ttacaggcga atagtcgttc gccgaagggt
    10381 taagaatata gccggcaatc ataatgtcgc ttacttttcc ttttatccga acatcacgca
    10441 tgaacgccca tgaatagagc tgcttggcat cgcaggttat tttttcgatt ttttcgtttg
    10501 agagaatatc ggcaagctcc gaagcaaagg tgaagctgct gttctcgacg acggcaatga
    10561 atnnnnnnnn nngcaatgaa ttcgccgcag tcgaaagcga ttgaaattat atcgccgtcc
    10621 gaaaattcgg gaatgaaata agccttatcg ccgagcctga tttccgagag cgactcaagg
    10681 tgcttgattg gaatgctgac tgtttcgata tcggtctgct cgcccttgcc gaggtcaaat
    10741 ttttcaatca ttttaaacat ttcaagcgaa gcaagaagcc ttgaggcctt gccgctgtcg
    10801 ggaacggacg gaatgtagaa atccatgtcc gtatcaatcg gagcgtccgt gcgaatggtg
    10861 ccgagcgtgt ggctgagcat tgcgctgtcc ttggaattga cgagcttttc acggagcttg
    10921 tcctttattt caattgtatc aatgttactg taaatgtttt caaccgagcc gaagcgctga
    10981 attaaatctc ctgcggtctt gggacctact ccggcaactc cgggaatatt gtccgagctg
    11041 tcgccctgga gagcctttat ttcaatcatc tgccacggtt caacgccgta gtcctctttg
    11101 atttttgctt tgtcataggg gattgtctga gttttgccca tcttggttga agcgagaaga
    11161 acggtcgtcg tgtcgctgac aagctgtaag ctgtcacggt ctcccgttgc tatgaagcac
    11221 ttgtcaccgt ccttcatctg aaccgaaagc gtgccgagaa tgtcatcggc ttcccatccc
    11281 gctttttcga caaccttgta gcccatgagc gtcaaaagct ccttgagaac gggcagttgc
    11341 tgcttaagct cctcgggcat ggcatggcgg cctgccttat attcgggata catttcgtga
    11401 cggaaggtcg gcttcttgac gtcaaaagca acggcaaccc tgtccggtcg gcaatcgtcg
    11461 agaagcttat gaaaaatatt catgaagccg taaattccgt ttgtatattt gccgtccttg
    11521 gtgctaagga gctttattcc gtaaaaagca cggttaatta tactgttgcc gtcaataaca
    11581 agaagtcgca ttttatttca ccttacttaa tttagcgttg attttgtcgc ggtcgggtgt
    11641 gacgttgacc gagtagtaaa cgtggtatat gaccttaccg tagggtgcac cctgcggctt
    11701 tttgagattt ttaacatatg tgtagtcaac cggtacaagc tttgcatcgc gagcctttga
    11761 atgataggcg gcgatttcgg ccgcttcaac tatcgcttcg tcgcttatct ccctgttgtc
    11821 gctgacaatt ataacgtgag agccgggaaa tttctgcgtg tgaagccaca tatcattttt
    11881 tgcggccgtt ttaagcgaaa gtttgtcgtt ttgcacgttg ttccgcccga cgagaaccct
    11941 aaagccgtca gttgtcctgt attcaatcgg cggcaaggcc tttgatgatt ttttatcagt
    12001 tccacgcttt ttcttgacgt aaccgccaag cgaaagctcg ttacgaattt cgtcaatttc
    12061 agcctgcgtg tcggctcgct cgagtgaatc cgcaaccgtt tcaagatagg caatctcctg
    12121 cttgctctcc tcaatgaagc cggtcaaaag ctgctcggca gtcttcgcct tgcgatattc
    12181 cttgaagtat ttttgtgcgt tctgcgacgg ggattttgac gggtcggctt taatcctaat
    12241 cggtttgttt tcgtcatagt aattttcaag gtcgtagaaa agcgcacctt tttcgagtct
    12301 gtaaaggttt gcgttgataa gctcggcatt gattctcagc ttttctctgt ctgcacagtg
    12361 agccagctcc gcctgcctca gactgagctt ttttgaaact ctcgatattg cggaattgag
    12421 aagctttacc aagtcctgcc ccttttgctt tgtgcgttcg agtcggtcac attcatagta
    12481 gaactcgtca agaagctcgg aaaaacttga ctttatctcc gttctgtagc ggtcgccgta
    12541 ctgttttatt ggaaaaaaag agaagtcaac gggcttgcct gtctcgtcct tgagcatgaa
    12601 aggaacgccc ggttcattca taatgatatt gcgtaaattg tcaagatttt cggagagata
    12661 ctcaaagccc ttgtcgctga cctcgtcggt gtaaagctca ccgaactctc cgcaaagctc
    12721 acggcaggtg agaggagagg caccctgcaa ggtgctgaga acggcagacg aaagagactt
    12781 tgaacggaag cttttaatct gtgcgataat gttctccgtc gtgtgatcct ttatgttaag
    12841 cttgctctgc ggcggcggtg caatatattt gaagccgggc agaatctgac ggaccgccga
    12901 ctggctgagg tcaacacgtt tgattgcgtc aacgattttt ccgtcgccgt cgaagagaat
    12961 gatatttgaa tattttgcca ttatctcaat cgagagaatg agttttattt tatcgccgat
    13021 ttcgtttgtg gcattgaagt cgaggtaaag tatacggtca agacccgatt gacggatatc
    13081 gacaagctga gcgttgagca gcttttttct catcagcatg caaaacatcg gtggaacagc
    13141 cgggttctcg ggcgcatgtt cggtcagatg gattctcggg ctgttgcccc ttatcgaaag
    13201 aaaaagcttc ttcgctccgt ttttgccgcg caggtgaata accagctcct cacgggaggg
    13261 ctggtggatt ttttcaactc tgccgaatga gatttctctg agcagttcgt ctttaatttt
    13321 gctgagaaat attccgtcaa gtgccataat tacctcaaat aaattttatc ggattttcct
    13381 gtttaatatc tataatttca atgtcgggga actgtgcctg caattttctt acaagaacgg
    13441 gaacaacaac tatttcagtt tcgtaatgtc ccactgcgat gagcgttatn nnnnnnnnna
    13501 gaaagttatg atgcgatgcg tcgcccgtta tgaaagcgtc aatttcacct gccgcttcaa
    13561 tcagcgaaca gcctgcgccg gtgcagattg cgattttttt aattgttttg cctgcgtcgg
    13621 catagcgcac cttcgtgttg agcgtttttg cgattttgcc tgcaagattt tccgccgtgg
    13681 tcggctcaac cgttccgagc cttaatatcg gatcttcggt acgggtgacc tctattccga
    13741 gcagtttcgc aaggctgtcg ttcgttccgc cgtctgcaat gtcgagcgga gtatgacagc
    13801 agagagcgtt tatcgagctt gcgacaagct catacggcac actgcccttt gttacggatt
    13861 tgatagggtt gaaaatgaca gggtgatggc tgattattaa gtccgcaccg attgctttgg
    13921 cttttttgat ttcttcgtga gtgatatcga gcacaacggc aacctttttt acttcggcgt
    13981 ccatatcgcc gacgagcatt ccgctgttgt cccagtccac ctgcgtttca aagggagcaa
    14041 tgctgttaag ataaccgtaa aaatccttta cctttgccat ttttccacct cttttattat
    14101 tgcttcaagc cgctcttttt cgccctcgtc accgccgtgt gcgttgagtg cagaaaggcg
    14161 agnnnnnnnn nnctccgata catatgtaaa tttttccgtc ctcgaagcag accgtctcgt
    14221 ctgttatatt aaaaccgttg gcaaaaaggt atctgcgaac gtcctcgtca tgcgactgcg
    14281 gctgtagaat aagcttgtat ttttcgtttt ttacccattc ggcggctgtc agaatttcgg
    14341 agatcaaaat accgcccatg cctgcgataa caatatcgtc ggcatcatcg ggggaaacac
    14401 atttaagccc atcggaaagg cgaagctgaa ccctgtcctc aatatgattg gagcggacgg
    14461 tttccaaggc gttttccaat ggaccttttc tgaggtctgc cgcaatggca gacggtattt
    14521 tgcccgacaa aatgagcgct gtcggcagat aggcatggtc ggttccgatg tcagcgacac
    14581 gggaaccgta acgaaccata tccgccgccg attgaagcct cggcttaagt cgaatcattt
    14641 gcttgcaaga taagcgttga tgtctgcaac catctgctca acgtcaacac cgtgaacggc
    14701 gcatgccatc tcaaggctct ctcctcttgc cgaggggcag ccgagacagt gcatacccat
    14761 gttgaggaag aactcggctg tgccgctgtc tgcatcaaga atatcgccta taagcatatc
    14821 tttagtaact tctgtcatgt taaaacctcc gtttcatact gttatatata ttatacacat
    14881 tatttattat ttttcaaggt taaatgcctt tatactcttt tcaaaagggt aaaaatatgt
    14941 tagaataaac cgaacggagg gttaaagata tgaaaaagtt tttgttttat cttgtagaat
    15001 ggacatgggc gctgccgatc aaccttatcg gcttcattgc ctatataatt tttgcggtca
    15061 tcaagggctg taagcacgaa agatttttca atgcgacggt tacatannnn nnnnnngccc
    15121 gatatggttc ctcgtcattg cgattccgtc atttatctgg tgcaatttta agccctgcat
    15181 aaactaccga aagaacaaca acgtttctta ttactccctc tattgtgaag cttgggcaaa
    15241 cgcatgggga cagaagttca caggcctcaa gcagacgaga ataggaaagt gagtattgaa
    15301 gataaattca aggtgcgatg tgggcatcgc accctacgag ttagttatga caaaggcacg
    15361 tttcgtaggg gtagatgacc acatcgaccc aaagtaagaa gaaaccaaat tcggcgatcc
    15421 tatatgggaa caagagatga tttcggcgga tttgaaataa attataatag tttcaaatcc
    15481 ccaccaccgc aagcggtccc cctccccttg cttcaaggg
//
LOCUS       DAWBSW010000024        15247 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_850_length_15247_cov_2.595642, whole genome shotgun sequence.
ACCESSION   DAWBSW010000024 DAWBSW010000000
VERSION     DAWBSW010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15247
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_850_length_15247_cov_2.595642"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3290..3299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3877..3886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9012..9021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9625..9634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3927 a         3797 c         3107 g         4376 t
ORIGIN      
        1 gctggaaacc gtaatactgt catagccgtc aattccgcag tttttaagaa cctttaagca
       61 ttccctatag cttctgacgg aacggttaca aaaatactca aagaacattc tttttctgaa
      121 atccgatccg atttcttcct tgtcccactt ataataatcc gttggggcga aaatattacg
      181 caagccgccg acaatatctc ctgttcttgc aaccgactgc tttttttgcg agacattata
      241 atactctatg ccctccggca gagctctcat aatcggctca ccgggatcaa gctcaatggg
      301 caatattata acacgctcaa agtgatcctt caaaaaggct acctcggatt caatataggt
      361 ttcacttata ccgtaaggga aaccgcttgt tatcataatc aggcatcttt tcaaaaaatc
      421 acctccacaa cgctttattc tatcatttta caggtaccgt gtctacaatt tttttaaaat
      481 tcctgcaatt tccacgcttt atctgcaaat ttcggcaatt acctctgtaa atatcgctgc
      541 cgcagtcaga atatcttcaa tcggcataaa ctcatttgca ccatgcatat ttgtcacagt
      601 gtcacggctt atagcgccga aagcgactcc gttctctata tcgtgaacgt aggttccgcc
      661 acccatgctg atacattcgc ccttgtcgcc gctgtaatcc tcataagttc tgagcaaggt
      721 ctttataaaa tccgagtttt cgtcaacgta atgtgcctct ctcattttcg tattttcaat
      781 ttcaaagcca tgctttctca ggcttgccgc aataacatcg gcacagtttt cctttgtcgc
      841 cgacatcggt gttcttgtat caaagcagcc gtcgaatttg ccgttttcaa atctcacgac
      901 attcaaagtc agtgtaagcc gcccgagcat atcgctcata tcgacgccga gcgcctcgcc
      961 gttatatttt ccatgtggaa acagagcttt aagactgtct gcaaattccg tacattcgct
     1021 gtcaaaatca atatagctca gcaattcaaa aagtgccgtt atcggattgt taccgttctc
     1081 cggaagcgaa gcatgagccg atgtacccga gcaaatcagt ttctcaccgt caaatgtaaa
     1141 ttcggcattc gtgtgctttg ctttctctgc ggcaatatca atttcctctt ttgtcactcc
     1201 gataagatcc gcttttgcaa aagcgggtac ggcattggcc gcttcgccgg catcaatgct
     1261 ctttatgtat ttcctgcagt ttgaaaaatc aaccgtcctg taaaaatgct ttgtaaatct
     1321 tcccttttct ccattcgtaa cggggaaatt cgcatctggc gtaaacgcat atttcggata
     1381 cttattcttt gaaaaaaaat atttgaggtc accgcttccg cattcctcat cggtgccgag
     1441 aatcaatcga caattcttgc tgacgggcag attgaggtct ttaaccgctt tcatagcgta
     1501 aagcgccgca acagcgggac ctttatcatc actgactccc ctgccgaaaa tcataccgtc
     1561 cttttcaaca acggtataag ggtcggtatc ccagccctca ccggccggca caacatcaag
     1621 atgcgccaga acagccagct cctgctcgcc gccgttaagg tcaaccgtac cgacgtagcc
     1681 gtcgcagctt gccgttttga atccaaagct ctctgctttt ttgagagcaa gcataagagc
     1741 ctcatacggc tttttgccaa aaggcttgcc ctcctcggcg gtaccgcgta cgctcggcac
     1801 agcgacaagc tcggatattg cttttatcat ctcatcttta tttttcttaa agtaatcaat
     1861 tatttctctt ttcatcagaa ctcacactcg gcattacatt tttctgtttt aacaagcgat
     1921 cctttttcaa ccgttccgtt ttctgctccg caccacggac aatagatatg atcctgcggt
     1981 gcggtaagat cgggattgag tggaacattc gtgtttctga caacttcaaa acgtttatag
     2041 cagcattcgc attgcttttt cattatgtac acctctgtta caaaaaagag ttgccgaccg
     2101 aggaaaaatt gcacacttcc cccactgcag caaccccatt taatttatat ctctttcttt
     2161 tcttatctgc ggtcaccgaa aaggctctgt cttgcctttc tgatctcggc gatgttcgac
     2221 gagagaacct catcgctgtt cttcataata tcctcaacga actctgcggc cgccgagcga
     2281 atttctccgg cccacttgtc cgccttgctc ttcgtgtctt gagcctgatt gtttgcatca
     2341 agaataattg catccgcctg gcttcttgcg tttctgacaa tgttctccgc ctcggctgta
     2401 gcctgagcac ggacacggtc agaaaactca tgggcaagtc ttgtgatctc cgagctttcg
     2461 atcatctgct ttgcctgctc cgaagcagcg gaaagagttg actttgcgtt tgcctctgcc
     2521 gttgcggcaa ttcccgaagc cttttccttt gcggcgctga ttataccgtc cgcctccgtc
     2581 tgagcggaag aaacgattga cgaagcctcg ttatttgcct ttgttataag gttattatgg
     2641 tgagccacta cctcctgagc cttttcaagc tcaatcggca aatcatatct gatgtcctcg
     2701 atgcattgct taatggtctc tgcatcaacc gcaacctttc ccgaaagagg aattttgctg
     2761 cccatgtcaa tggtatcttc aattttatca agtaagccct gtacgttcat cgttagtctt
     2821 cctttcttct tattccttta actatatcgt cgagcacctg ctcgggtaca aagtccgaaa
     2881 cgtcgcctcc aagctcgcaa acctgcttga caatgctgga cgaaagatac atataatccg
     2941 cacctgcggt gacaaacact gtttcaacat cgggattaag ctgcttattg gtaagtgcct
     3001 gctggaattc gtcctcaaaa tccgaaacgg cacggagtcc ctttacgatt gcgtccgctt
     3061 ttttgattct tgcatattcg gcaagcagtc cgtcataaaa atcaacctca acgttcggaa
     3121 gattcttgac acatctttcg atgaattcca ctcttttttc cggattaaag cagccgacag
     3181 gctttttggg atttatcatc acaacaacaa tgactctgtc aaaaagcttc gatgcacggg
     3241 ttataacact gaggtggcct tttgttaccg ggtcaaagct gcccggacan nnnnnnnnng
     3301 acatattgcc gttctcatcg gtcgtcctcc tcgctgatac gatagaccgt tataccgatt
     3361 tttccgtatt tatagttacg gtaaattgag aaattgccga ccgtctgcgg catctcctct
     3421 ccgtaggggt gttcgcagat tatcacaccg ccgtcattaa cattcttaac gacatagggc
     3481 agggtattct gcaagagtcc ctttgagtac ggaggatcca taaaaacgat atcgtatttt
     3541 tttctgtttc ctttaaggaa cgttactgcc tccgtgcgaa taacctcggc attgttctca
     3601 agcttggtcg agataagatt tttctttacg atatccacac tttcggcgct catatcggta
     3661 aacgtggcgt aagccgctcc cctgctgagt gcttcaattc cgagctgacc cgatcctgcg
     3721 aaaagatcaa gtacacgcct gccctccagt tcgaactgga taatgctgaa aatcgcttct
     3781 ttaaccctgt ccgttgtcgg acgaacgtcc tcgccctcca aggttttgag aacccgtcct
     3841 cttgctgttc ccgtaatcac acgcacggcg caagctnnnn nnnnnnacta attttatata
     3901 catcttatta tcacattttt taccttgtgt tgtcaacatt ttttctaaaa taatcataaa
     3961 ctttttgcgc cgtggctctg tttatcccct caacacggca aagttcgtca atttccgcat
     4021 tttttatcgc cgtaacggtt ttgaaatgct tcagaagagc cttggctttt ttctcgccga
     4081 ttccgtcgat ttttgtcaaa gttccgccga gagacgattt ctgatgtttc gatcggtgat
     4141 atgaaattgc aaatcggtga acctcctcct gaatcgtcga aacgagtgta aatgcctgtc
     4201 gctttgagtt gattgcgatt gcgccgccgt catatgcaat ggccctcgtt ctgtgcttgt
     4261 tgtccttaac catgccgtaa acaggaatat cataaccgaa cgcacgaaca ataggcaaaa
     4321 ccgcattgac ctgtccctgt ccgccgtcaa gaagaataag atccggcagg attccgaacc
     4381 cctcgccgct ttccttctct tcttcatatc tgttaagccg acgtgaaata acttcagcca
     4441 ttgagccgta atcgtcctga ccgtcaaagc cctttatcgc aaaccggcga tatgattttt
     4501 tgtaaggcaa tccgtttttg aaaacgacca tgcctgcaac gttatcgctg cctgccgtat
     4561 gcgaaatatc gtaggattcg atcatcacgg gcggcttcgg caaattaagg agccttgcaa
     4621 gctcgtcaag agccgccgta gcctttgcat ttctgcccat actcaaagca agcttctgcg
     4681 ctgcattgtt catgcacatc gtcatgattt caacctgcct gccacgctgc ggaacgttga
     4741 tctcaacctt tcgtcctgca ttttcgctca gccattgcac aagcaattct ttatcgtcaa
     4801 cctcaccgtc aaccgtaata aacggaggaa tatgtctgcc cattgaatag aaacgggtta
     4861 taagctcacg tctcgcctcg ggcagggtat ctatttcatt gaataaaaac cattcgttat
     4921 ccctgagtct gccgtcatag aaacgcaaaa cggaaagaca gtatctttca tcggactgtg
     4981 cgattgcaaa aacgtcctgc tccctgacat ccgagccaac gaccttttgc ttttcattaa
     5041 cttttttaat agaatttatt ctgtctctca gctttgccgc acgctcaaaa tcaagccttt
     5101 cggcggcgga ctccatttct tccgttaatt ctttaattat ctttgcgctg ttgccctgca
     5161 aaaaatcgag agcggcattg acagattcgt tgtattcggc gcagcttatc ttgcccgcac
     5221 acggagctga gcattgcttg ataaaataat tcaagcaagg tctgcctttg ccgatttcct
     5281 tcggaaagct ttttccgcaa cgcggaagca tgaaaatttt ctgcgcctca tcaacggaat
     5341 tggttacgct gaaggagctt gtatagggcc cgatatagcg tgctccgtca tccgcctttt
     5401 gcaacactga gctgattttc ggccacggtc cctcactgac tctgatatag ctgtagccct
     5461 tgtcatcctt cagaagaatg ttatatttag gcgaatactg ttttataagc gaacattcaa
     5521 gcacgagcgc ctcaaactcg ctgtcacaaa gaatatagtc aaagtcaaat acattttcga
     5581 ccatctttct gaccttgatt gagtgattat gctgtgagcc gaaatattgg ctgacacggt
     5641 ttttgagctt tttcgccttg ccgacataga taatctcgcc cttggtgttc ttcataagat
     5701 atacacccgg cgtcagcggc aaagacattg ccttttttct caactcatga atgtccacgg
     5761 tattcacctc gattccgtaa attctctata aattcaaaaa ctgccgcaca tcatatgcgg
     5821 cagtcacggt ttagtaaggg attcttatac tgcaaagccc gagttaacaa gcttaacaag
     5881 ctcatcaacg gcctgctcct cgtcagggcc tgcggcgatg actttaattg ttgtgccgcc
     5941 gtcaataccg agagaaagta caccgagcaa gctctttgca ttcacacgtc tttcttcctt
     6001 ttcgatccag atagatgact tgaagcaatt agccttctga ataaaaaatg ttgcaggacg
     6061 ggcactgaga cccacctgat tctgaactgt aacgtctttt tcacacataa ttgatatctc
     6121 caatcaaagt taaatccaag tctgttttca gcctttcata caacagttat tatatcacaa
     6181 aagttgtcat cgtcaacaaa taaaattgac tttaaaatta tttcggcttg tattccaaat
     6241 gtgagtattt tcagaagtca tgctttgtgc actttgtaca ccgattaact gtacttttaa
     6301 tgcacattga ctatttttta ctctcattat ccaacgattg gagatatttc atgtcttttt
     6361 cacttaattc ggcttttttc agcaaatcgg gtctgcgtct ctttgtccgc tccagcgact
     6421 gctccctgcg ccacttcatt atattggcat ggtgacccgt caaaaggaca tcgggaacgg
     6481 ttttttcgtg ccattcatac ggtcttgtgt actgcggata ttcgagcaag ccgctatagt
     6541 ggctctcctc ctcaaacgct tcatcgctcg caagcacacc gggcaacatt cttgttatgc
     6601 tgtcggcaag tacgagagca ggaagctcgc cgcctgtcaa aacataatcg ccgattgaaa
     6661 tttcttcatc gacaagttcc tcgataaccc gctcatcaac accctcataa tggccgcaga
     6721 gaatggctat attatccatt tttgcaagct ccttgactct ctgctggtca agtgttttac
     6781 cctgcggaga catatatatc aggtgcggac ggctgccgat ttctctttca agctcgccga
     6841 aacaatcaaa tatcggctga cactgcatta tcattcccgt gccgccgccg taggttgtat
     6901 catccacacg gcgatgtttg tccgttgaaa aatcacggat attgacacag tttatttcaa
     6961 ccagtccgct ctctctcgag cggccgataa tgctctcgtc gaggacacgc ccgcacatct
     7021 ccgggaacaa tgtcattatg tcaattctca tcgtcaaata tacctttcat cggtcttatc
     7081 agcaccttgt tttccttaac gtccgtattg atcacaacgc tcggaattgc gggaataaga
     7141 tattcctttt caccgtccgc cgcaatgtgc caaacgtcat ttgcacccgt ttcgctgacg
     7201 tcggaaattt ttccgtatcg cttatccgta tcatcggcat caaaaacatc acagccgata
     7261 agctcctcga tgaaataaac tccgtcggga agccttgcgt ccgagcggcg catataaagc
     7321 cgcttgtttc tcatcgccga agcagcttcc acagtgtcaa tgccctcaag cttcatcagc
     7381 acgatgtttc cgtgcggacg tgcggaaata actccgacac tgtttttgcc ctcggcgtca
     7441 taatacaatg ttttgaattg tttaagaaat tcgggtgagt ccgcccacgg gttaacacgc
     7501 atttcgccgc gcacgccgtg agttcctacg atctgaccga ctgcaagaaa ttcttttatc
     7561 ataaaatcat ttaacctctg tgaaatatct tacaaagctt ctgcctgcgc tcaacgttcc
     7621 gtcagtcagg ttgatacctg cgttcataag caatgcaccg gaataaatct caccgtctgt
     7681 ttcatcgcgg tacatctttt cgggatcaag tcccttcatc ttaataagca gaaaatcgtg
     7741 cggcgctctg ttttttataa caacagtcag caaggcttca ctcttatcct ttgcaacata
     7801 ttcccatgca cacttgaact catcgtccgt cggtgcaata attctgtaaa gatcgccgaa
     7861 gtgaattaca ttataatacc tgtgataatt cttaacctgc tctttgacct tttccttatc
     7921 ctcctcggac atctccttgg ggtcaagctc ataaccgaag gtgcccgcca ttgcgactgc
     7981 cgccttggtt tcgattcctg ttctgtcaca ggcggaaaca tgagcgccca tgctcgatgc
     8041 cggatagcac attgttgtgc cgaactgaat tgtcagtcgc tcgatcgggt cggtgttgtc
     8101 cgaagcccat gcctgcggca tatagtaaag cataccggca tcaaaacgtc cgccgccgcc
     8161 ggcacagctc tcaaagagga tatgcggaaa tgcgcttgtt attctgtcca taagctcata
     8221 tgtgccgaga ataaagcggt ggaaaaactc ctttgaatga cgcgcatcaa gcatcaccga
     8281 cgcaggctct gtaatagctc tgttgtagtc ccatttgaga tatgcaatat tgttcttcga
     8341 aagaacatca tacatctgat tgaatatgca atcacgaaca tcctgtcttg tcatatcaat
     8401 aacatactgc tgacgggcag gcgactgctc tctgccctca acatgaacgc accagtccgg
     8461 gtgagcctta taaagctccg aatcctcgga aatcatctca ggctcatacc aaatgccgaa
     8521 ctgtacgccc tctgcattta ctctttcgat aagctttgtg aggccgccct tgagcttgct
     8581 ttcattgaca aaccaatcgc cgagagagga attgtcgctg tcacggtgac cgaaccagcc
     8641 gtcgtccata acgagcagtt cgattcccag cttcttagct tccttggcat agcggacgag
     8701 ggtctcctgg tcaaaatcaa agcccgagcc ctcccagctg ttgataagaa gcggacgctt
     8761 tttatttttc cattctcctc tgatgagatt gttgttgtaa agatcgtgga atgtgcggct
     8821 cattttgccc atgccgctgt cggaataaac cattacgacc tcagggcttt ggaactcatc
     8881 gccgggttca acgacccatg tgaaatcaat agggttgata cccattacaa ggcgtgtgca
     8941 gtcaagataa tccgtctcga tatcagcgga gaagttaccg ctgtatacaa ggttaaaacc
     9001 gaaagcatca cnnnnnnnnn ncatcaccga aatcctcgcc gcctttatca tcgacaagtg
     9061 caacaaacgg attgtggtta tggcttgacg aacctctttt gctggcaaca gactgtattc
     9121 cgtgagcaag gtgccgtgtt atgactcttc tctctctcga ccaaacgccc gaaaggttta
     9181 tcatgttata atccatcgac gggaagttta cacagcagct tgcaaccttt tcaatctcaa
     9241 aggtttcctt tcccgtattc tcaaccttga cgctctcggt gacaacgctg tagtttgcaa
     9301 atgcggtgta ataaagcgtt acctttgcgc ccgtgaattt atcaattgta ataagctcaa
     9361 gcgtctgagc ctcactgtca tcattgcaat aagttgccgg aagtccttta agcttcggct
     9421 ttccgtcgta gattttatgg tcaagatagc gtatatccgt cgttgttctt cccattgaat
     9481 cctttattga aagtgcggca agtctgtaat cgcccgaacc gttacaggaa tactccatcg
     9541 gctgaacgtc aagagaaaac ttataatcgt caacatgaac gtttttcggc gaaatagtgg
     9601 caaaatatcc tctgaacggt aaatnnnnnn nnnntcatcc ggaattttct ttccgtaata
     9661 gagatgaata agatatccct cacggattcc gattgcatat gttgaatcaa gcgtatcaag
     9721 cttgaaaatc tttctttttt catcaaatgt aataggcata gctaaacctc gcttatttta
     9781 tcgttatgtt atattttatc atatactgag ttgcaggtca acaatttacg attgacaaat
     9841 tcacttaatt actataaaat tttatcatag aaaaaaacgc acagaagagg tgttcaccac
     9901 ggaaaagaaa aacatacata aggaccatcg tcaaagggta agagattcct atctcaaaac
     9961 aggcattgac gcaatggcag atcacaatat tctcgaactt cttcttttct attgtatacc
    10021 tctgaaggac accaatccga tagcgcatga gctgattgaa aaatatcacg atctcaacgg
    10081 cgtgcttgat gctccgataa aggagcttga aaagataaac ggaatcggcg aaaatgccgc
    10141 cgtaatgata aagctcatcc gtgacatttg ctcaaaatac catgacaatg cgataaacaa
    10201 caaggtcaat cttgcctcgg cggaacgtct ttacgatttc ataagaatga aatatctcgg
    10261 agaaacaaga gagatcgtct acatgctttc gctcgacgct cacggcaggc tcaagcattg
    10321 catcaaggtc accgacggaa caccgacaac cgcggtttcc gataacagaa gccttgttga
    10381 gcttgcgctg agatttgaca ttacaaacgc gatcatcact cacaatcacc cgaacggatt
    10441 tgccactccg tctcaggcgg atattgtcgc gaccgaagcg attgcgaagc ttttttcaac
    10501 ggtcagcatc aatcttgttg atcacgtcat cgttgccgag gacgagatat tctaatttgc
    10561 gatgagcaag aagtattcat catttttagg ataaagccat ttaatccgaa aggagattga
    10621 tttatatgaa aataattaca gtaagccgtg aattcggaag cggcggtcgt gaaattgcca
    10681 aaagactttc cgacgttctc ggaattgcct attacgacag cgagattatc accgaaattg
    10741 ccaagaaagc cgaccttgat gaaaactatg tagcacattc tttggaaaaa ggcattcagc
    10801 cgacgtttca gattcacttt gcatgtgctt tttctcacat ttccgctccg gatccgacgg
    10861 ttaaactgct ttccgctcag cacaacatca tcaatgagct tgccgaaaag ggcgattgca
    10921 taatcgtcgg cagagccgca gatgttttgc ttgaaaaata cgatccgttc cgtatctttg
    10981 tttatgccga tatgcagtca aaaatcgcaa gatgccgcaa gcgtgcaggt gacggcagca
    11041 agctcacgga caaagaaata gagaagaaaa tcaaacagat agacaagggc agagcctcaa
    11101 gctacaacat gatttcctcc accgactggg gtgacaagcg cggctatgac ctttgtgtta
    11161 acacaacaaa tatgaatata aaggagatta cgcccttcat tgccgaatat gcaaagcagt
    11221 ggtttaagga taagcaggca tgaagataaa actttcggat cactttactt attccaagct
    11281 tttaagattt gtatttccgt caataataat gatggttttc acttctattt acggagttgt
    11341 tgacgggctt tttgtctcaa actttgcagg caaaaccgcc tttgcctcaa tcaatctcgt
    11401 tatgccgttt gtaatgatac tcggaggcat cggcttcatg ataggcactg gcggaacagc
    11461 gcttgtctca aaggttctcg gcgagggcaa gaaggaaaaa gcgaatgaga ttttcacaat
    11521 gatgattatt tttacgcttc ttctcggcgc tttgctctct gtcatcggcg ttatttcaat
    11581 gccgtgggta gcgaagttcc tcggcgcaac cgaggagatg atggctgatt gtgttcttta
    11641 cggcagaatc gtaacaggct ttaccgtcgc attcatgctt cagaacgttt tccaaagctt
    11701 tctcatcgcc gccgaaaagc ctaagctcgg acttcttgca acggtgctgg caggcattac
    11761 gaatatggcg cttgatgcga tatttataat tgttttcaaa tggggcgttg caggtgcggc
    11821 aatcgcaacg ggtctgagcc aatgtgttgg cgggattttc ccgttgattt atttcttgag
    11881 gaagaattca agcctattgc gcctgacaaa aacgaagttg gaaataaaac ctattctcaa
    11941 cgcctgcggc aacggctcct cggagcttat gagcaatatt tcctcatccg ttgtcagcat
    12001 gatctacaac ttccagttga tgaaatatgt cggtgaggac ggcgtttccg cttacggcgt
    12061 gctgatgtat gttcagttca ttttcgttgc catttatatc ggatatgcga tcggctgtgc
    12121 gccgataacg ggctaccatt tcggagcccg gaaccacaat gagctgaaaa atatgctcag
    12181 aaaaagctca tttctcagcg ccgtttcggg cgttgtttta acaatactgg cgattgcgct
    12241 ttcatcgccg ctggcgaagc ttttcgtcgg ctatgataaa gagctttatg aattgacccg
    12301 tcacgcattc agattgttcg cctattcatt cctgctggca ggcttcaata ttttcacctc
    12361 atcgtttttc acagctctca acaacggtgc ggtttccgca gcaatatctt tcatgcgaac
    12421 gctgattttt caaacctcgt cggttcttat acttccgata tttctcggcg tggacggtat
    12481 atggtgggca atcacggttg cggagatttt cgcgtttatc ctctccctca tcttcctttt
    12541 tgccaagcgc aagaaataca attacatata ataacggcat ataaatataa ttaaaaaagc
    12601 agaaatcttc cgttttggaa aatctctgct ttattatttg ttgattaaag aaccttgttg
    12661 aagaaatcaa ttgccctcgg ctctttcgga ttgaggataa cctcctgcgg cgtgccttct
    12721 tcgataatat agccgtcgtc aatgaaaagc actcggtcgg caacctcacg ggcaaagcct
    12781 atctcatggg tgacgattgc cattgtcatt ccctctgccg caagatcctt cataacctgg
    12841 agaacctcgc cgaccatttc ggggtcaagc gccgatgtcg gctcgtcaaa gagcatgata
    12901 tcgggattca tgcaaagaga acgtgcgatt gcaactctct gcttctgtcc gccggagagc
    12961 tgatgcggat aagcctccgc cttgtcctca agtccgacct ttttaagcat tgccattgct
    13021 tcctcttttg ctttttcctt tgaagccttt ttaagctgaa cgggagcaag cataagattc
    13081 tgaataacgt tgagattgtt aaaaagattg aagtgctgga acaccattcc gatgttctcc
    13141 cgaatcttgt tgaggtcggc ttttttatcc gtaatcgcct gaccgtcaac aatgatctcg
    13201 ccgcctgtcg gctcttcaag acggtttatg cagcgaagaa aagttgactt gccgctgcct
    13261 gacggtccga ggataacgac aacctcgccc tcatatatat cggtcgagat atccttgata
    13321 acctccaaat gaccgaagtt ctttttgagg tgagaaacgt gaattttaac gttgttttta
    13381 tttacggcca cggttcatcc tcctctcaag agcctttgca accttggaaa gtatcgtgat
    13441 aacgataagg tacatcacgc cgacaataag ccatacctgg aacgacttaa agttaattgc
    13501 aatgacgttc tttgcggcat ttacaagctc ggggaagccg ataacggaaa gaatcgaggt
    13561 atccttgagc gtgataataa actgattgat gatgctcggt atcatcgtgc ggatagcctg
    13621 cggaagaacg accttctgca tagatttcca atagggaata ccgagactcc ttgctgcctc
    13681 catctgtccg acgtcaacgg actgaattcc ggcacggatg atttccgaca tatatgcgcc
    13741 gcagttgagc gaaagacaga ttatacctgc ctgcaatgcg gtgaatgtaa aggtactgtg
    13801 taaaaagttt ttcaaagcgt aaggtacgcc gaagaaaacg aaaaatgcaa gaactatcat
    13861 cggaacaccg cggataatat caacaaagac ctgtgcgatg acgttgcata ccttactttt
    13921 aagcacgctg agcataccga aaatgagacc gataatgctt gcaaaaatca ggctgtaaaa
    13981 agccatgata agcgtctgtc ccattgcctc cagcaacagc ttgccgtaaa ccgaaattat
    14041 atctgccatt tagcggactc cttttgacaa acagcagggc atctttgcag acgccccacc
    14101 gctcattttt tcaaattagc caacgtactt gttgatgatt tcgtcatact tgccgcttgt
    14161 cttaatgttc ttaagacctg cgttaaacat atcgaggaat tcctgatttg ccgaattgaa
    14221 gatagcaaga ccgtaatcgc tgccttcgct ggctgtgttc tcaagaacct taagtgcaag
    14281 attgccgttc ttgatggagt caaccatgat cggtgtatcc tcgaagcaag caacagcctg
    14341 accgccgata actgcctgat acattgtcgg agaatcctca aatgaagcga ttgtgaagcc
    14401 atacttatcc ttaaggctgt ttgcatagtc ggcacccatt gtacctgtct taactgcaac
    14461 ctgcttgccc ttgagatcct caaagccctt gattgtggaa tcagcagcaa ctgtcattgt
    14521 ctgtgtgcag tgataatatc cgtctgagaa aatccatccc tcgctcttac gcttctctgt
    14581 gatagaagca cctgcgatca tggcgtctgc ctgacctgac tgacatgctg cgatagatgc
    14641 gtcccagcca acgctgttaa gctcgtactt gaagccctga tcctcggcta tagcggcaag
    14701 gatctcaaca tcgataccaa cgagcttacc cgaagcatcc ttgtactcaa acggtctgaa
    14761 aactgtgtcg gtagaaatct tccatacttt ttcatctgtc ttgtcgtctc ccttggagcc
    14821 gcagcctgcg aaaaggcaga acattgtagc tacgcaaaga agaattgcga taaatttttt
    14881 catgaactca aatcctttca attattgatt cgcaaaataa tacagagata gtttaatcta
    14941 taattgcgaa ttagtcaagg ctaacaaagc ctgaaatagt cattatttta acattttttt
    15001 gcttttgaat cataaaatcg actttttgaa aaatttattt actattcaca ccgttccgaa
    15061 ccgccgtttg taaacattaa attccttcaa ccgatttcag taaagaacgt ataataatgt
    15121 ataatatagt attatgcaac ccgactgtcg gtaatgcttt gcaaaaaatc tttgactgta
    15181 acacgctttg cgccgcacac aaaaaaagag caggcgtaaa cctgctccga ggataagggt
    15241 attcaat
//
LOCUS       DAWBSW010000025        15155 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_858_length_15155_cov_2.479205, whole genome shotgun sequence.
ACCESSION   DAWBSW010000025 DAWBSW010000000
VERSION     DAWBSW010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_15155_cov_2.479205"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    792..801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2052..2061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3222..3231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3893..3902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4858..4867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6038..6047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9644..9653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10995..11004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11583..11592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12082..12091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12742..12751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13075..13084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4061 a         3368 c         3303 g         4303 t
ORIGIN      
        1 aatcaatgct gtaataaagc cggcgatcga ggcggcaaga acaattgcgg aggcggcaat
       61 tccgagaata tccttaaagc tcttatccgc tgctttttct tcctccgatt cttcaacaaa
      121 gcttgctatg cgctcgtagt cgcaaagaat aaacaaaagc ttgtccgcac aggttctgag
      181 aagctctttc ggtgtttcgc cgggctccga gatgagcttg tatgtctttg ttccctcggc
      241 attggcaacg gcaatataag cgcaaagcat atttgcgtcg tcctcggaaa caaacgggtt
      301 ggatatgtac acgccaagct cggtgcggtt cttttcccgt acatttcttg caatctgaat
      361 cgcaagtttt ttgatatccg ttacatcatc gggaagctca tagtccatat cgaccgtgaa
      421 aacggattta tcgtttttct tgaggaatga aataagatat tccgctccgt cgttccccgc
      481 caaagccact cttactgagt cggcgcttaa tttattcact gcacactcta tcagtgctct
      541 gtgacggttc tttaatgcgt gagacgcggc acaaggctcg ctaagctcgg caaagtagtc
      601 atacagtgag ccgagaatta tctgctgagc cttaatgtcc tcaacaggca tataaataat
      661 atgctgaccg ccggaggtga gcgcaaatcc gcagttgaga taatcggaag tcggaagaat
      721 tgttgctgct tcgggaaagg ttacagaggc tttgaaaaag ttctgcttca tcttcttggc
      781 atcgggtctt gnnnnnnnnn nttgacgaaa gcactgcgga aacttcgccg ttttctgcgg
      841 tttttgtgtc aagcgcagca caaagcattc ttttggttgc attgtacatt gccgactgaa
      901 ccgcaacaag aacgatgtcg gcggcaagca gactctgtga aattgcaacc agcattctct
      961 tttgcgacgt aaattcggca aatccgttac attcggggaa gaaatcctta acgcatttga
     1021 gaatgaattc cttgcccttt tccgcctcct gcgagtcctg attgcaaagt gaaaaaagtt
     1081 ttatgtccat tgttttgcac ctctgtataa gttattcaag cgtgaatttt tttgcatctg
     1141 caaagggatt gtccatgccg tttgcatcgt accacgcctg aggaaatttt tgattgttgt
     1201 tttctttgac ctttgaaacg tcaacggttt cgctttcgcc gtcgcggaca cgtctcgcca
     1261 ttacgacgaa gcgtgagtcc tcaaattcat agcagcaggt tgagagagta agaattttgt
     1321 ctccgggttt tatatcgact gcatcgtttg tataatagga acgggatttg acgtcggcaa
     1381 taaattcgtt gaatttttcg tccgagggga aattgaccgt tagatagggg aatgtgtatc
     1441 cgttatcctg cgaatcatag atattcgcaa taaatgcggc aaaaatcttc caggttccgt
     1501 cctcgtatac ggtgttgaat ttaaccgtcg ggtattcctt ataattctcg atttccttgt
     1561 agtaattcag gtgtccgaaa atttgaccgt cacgcatttt atgaccgtaa ataatcgtgt
     1621 tcgtgtcaaa attatcgacc ttgtttctgt aatcgacaaa aagtgtgccg taaacggaat
     1681 attcgtcgtt gaaattctta taaaggtatt cttcgttatc ttcaccctga actaccttca
     1741 tatcgttctc gtcaaggaga ggaatcttta atattccgac aaaatcgttg ttcttttcta
     1801 aaagatcctt gtatttggca agcacatgct tggatttgtt cgccatcatg gttgtttgtg
     1861 tatcgctttc gtttatcgac ggagtttcca gtgcgttttt attcttgtac ggctcaatta
     1921 catagtattt aatgagatat gaaaagcacg caataaagca aagcacggaa aggccgagaa
     1981 gagcctttct gaaaaacgaa agcaaagatt tttttatctc ttttttgttc atctgttttt
     2041 cctctcaggt cnnnnnnnnn ntctgttacc tctgtgtcgg tgttgcgctt gataccgtcg
     2101 tccgcatcct cgtcaacgaa tataacacgt gtgttggtgt tgtattcctc gcttcggtcg
     2161 atatcttttc tttcgccgtt gccgatgaga ctttcaaagg aatcaaggtc aatgtctatt
     2221 tctccgaatg atatcgcatc gttgtcaaat tcactgtccg ccgatccgaa ctcttgagcc
     2281 aagggtattt gattattcga ttcgattata tcttttttaa agctcggcgc acttcgaagc
     2341 ccgtcttcaa cagctttttt tgtcgcttta ccggcaatca gcggattgaa gtcctcatcg
     2401 cgtatgcttt gatcctcttc tgcggctgca aagcgctttt tcaaggattc aaaggagctg
     2461 tcggttaaat attccgttat atcgtcattt tcatccggca aaatcggaac aaggtccgaa
     2521 tttatgccga ttttattttc atcaggcaaa atgatccccg ctttctcggt ttatcttgtg
     2581 ctcggcttcc atgagcgtga gccggagtag ggattggacg ttcccttata gtcgtaccaa
     2641 acctgaggcc tcctatagtt cggattttcc tttgcttttg aagtgtttac gtcgtcactt
     2701 tcacccgaac gaagaagtct gccgataaca acaagtcttg tatctacctt tttcccaata
     2761 taattcgtgt atgtataatt acatgtcgaa agatttataa ttttgtccgt cgctttgaga
     2821 tcaactcccg ttgcgtagag agcacgctgg ttaacctgct cgataaagcc ggcaaagttt
     2881 gaatcggtca tattcggctc aataaagtta aacatatatc cgccgtcgtc cttggagtct
     2941 actgttgtta ggaacgctgt gtaaatcaaa aacgtgtagt taccgttcag ggtacaataa
     3001 cggataacgg gatttttctt ataccattcg atatcgtcat atctgttaag gtcataaaaa
     3061 gctgttccgc agctcatatc gtgaccgtag ataaccgtat ttttactgag cgtatttctc
     3121 ttgcactgat agtcggcata aacgcagccg tatcttgtat atgagccgta aaaattcttt
     3181 gtcagataga aatcgttatc cttgtgctga acgatcgggg tnnnnnnnnn ncgatgccgc
     3241 cgagcctgta gaaacgccgg ttgcgatatt gaacgccgcc aaagccgttg atgaaacaat
     3301 tgcaatgatc agcacggcgc aaagaagagt gaaaatattc ttctttgcct taatatttgc
     3361 aggcttttct tgctttgaaa caggaactgc ttcggttttt gtttcaattt tcgtttcatt
     3421 tttcgactcg ggctttgtct gggtgtcggt tggcttcttt tcaacaggct tctttgttat
     3481 agtttcaagc gagtcgaagt cggggaacga gtcaaaaata tttccgccgt agcccgtatt
     3541 atactttttc ttatctttat tttcagccat tttactcatc ctcaaagttt gttgtcgata
     3601 taaagtctta tcatattgag cgcattggaa gcgctgacgt atctgttata gcttctgtcc
     3661 ttgcgtgagg ttctcagctc cctgacccaa acgttatccc tgtccgcaag tccgatataa
     3721 accagtccga ccggcttgtt tgtcgagtcg ctgtcgggac ctgctatgcc tgtaacagaa
     3781 actgcgatat ccgctccgct gaccttcagc gcaccctgtg ccatttgtct tgcaacaggc
     3841 tcgctgaccg caccgtattt attaaggtcg tcctcgctga cgccgagaac acnnnnnnnn
     3901 nntttgcata agaaataatt ccgcagtcaa aaacctccga ggctccggga acgtctgtaa
     3961 tacgctttgc tataagtcct ccggtgcagg attccgcagt tgcgactttc atgtgttttt
     4021 ctttcagctt ttcaataacg gcttcttcga tacaggtgta gtccacgccg taaacaaagc
     4081 cgtcaagtct gctttttatc tcatttataa caggcttgca gagattttcg gcttcttctt
     4141 ttgtccttgc catcgccgtg actctcagca atgcttcgcc gtccttggca tacggcgcca
     4201 cagtcggatt ctcggcttca aaaaggtcgt tgaccctttc tgccattaag gattcgccga
     4261 taccaaaggt tcgaatatta tgagagacga taattcggtc ggaaaactgc ataagatagg
     4321 gtacggcaca attccggaac atcggcttaa gctctctcgt aggaccggga agaacaagaa
     4381 tatgaacgcc gtccttttca atcgccatgc cgggtgcggt tccgttatcg tttggaaaaa
     4441 tcacacagtc cttcggaagc atggcctgct tgaggttgtt ctgtgccatt ttcatgccct
     4501 tggtgctgaa ataggtcttt atcttctcaa ctgtcggctc gtgcaaaaac atttttttgc
     4561 cgaaaaattc acagcatacc tcttttgtca aatcgtctgg agttggtccg agaccgcccg
     4621 aaagaataat aatatcgctt ctttcagccg cttctttgag ctgggcgagc agcctttctc
     4681 tgttgtcgcc gaccgtgctt tgatgaataa cgcttatacc gagcttcgca agctcaatgc
     4741 ttaaaaactg cgcatcggta ttaagtatat cacccaaaag tatttcggtt cctacgctga
     4801 taagttcgca aactgccaat ttactcacct gttattctta caaatttaat attgttgnnn
     4861 nnnnnnntca caagggcttc aacatcaaga acggcttccg cttcctcaac ctgcttgaga
     4921 tgcggacgtg tcctcgggtg cttcggcgtg aaaaccgtgc agcagtcttc atagggaaga
     4981 atggaggttt caaatgtgcc gatatatctt gccctttcaa taacctcatc cttatccatt
     5041 ccgataaggg gacggaaaac cgggagatcc tcaaccacgg cttcggtaca gcctatcgcc
     5101 tgcattgtct gacttgcgac ttggcctacg ctttcgcctg tgataagcgc tccgcagccg
     5161 tttttctttg cgattttttc actgcatctc atcataaatc ttcgcataat gatcgtgaaa
     5221 agctcctcgg ggcacttatc cttgatttcc tcctgaatat gtgtaaaagg aacaatgtta
     5281 agcggaattt caccgctgta tttcgcaacc tgtttaagaa gcgagcagac cttcatttcc
     5341 gcacgctgtg aggtgtacgg aggggaggcg aagtgtacgc cgcaaaggct gagtcctcgc
     5401 cttgacatca tccatgctgc aacgggactg tcgattcctc ccgagatgag gattgcggct
     5461 tgtcctgcgg tgccgacggg cataccgcct gcgcccttga cctgattgcc gtgaataaat
     5521 gcgtaattat ctcttatctc aacgttgact atgatatcgg ggttgtgaac gtcaacggtg
     5581 agatgcggaa atgcgtcaag aattttttcg ccaaccgtca tacatatagc aggggagttg
     5641 agcggaaatt ttttatcgga acgttttgcg ttgactttga aggtctttgc aaaaatcagg
     5701 tcgtctttaa ggtattcgca ggcggcggga attataacgt ccatatcctt ttcgactgcg
     5761 gcacagcgtg aaattcccgc aattccgaat atcatcctga tccttctgaa tgcctcgtcc
     5821 aaatcgacat cctcgtcgat aggctcaacg gttatcgttg actgggatga aacatatctt
     5881 atttttccga gatcgtgaag tctgaacttc atatttttaa taagctgatt ttcaaatgtg
     5941 tgacggttga gacccttgag ggcaagctca ccgtttttca gcagaattat ttctttcatt
     6001 taaaatacct catagctttc ggcaaccgtt tcggccgnnn nnnnnnnttc tcattgtttg
     6061 gcgcgccgat gaaattccgc cgatcagcat gtcgatttcc tccttggtcg tgaaaacgga
     6121 ggtgctgatc ctcaacgccg tgcttataac gtcctcgctc aggttcatgg ctttcagaac
     6181 tcggctcttg tgtccctttg agcaagccga gccgctcgaa atataaatac ccattccgga
     6241 aagatagttg agcatgacct cggacgggat accttcaaca gaaatattcg ttatataggg
     6301 cagggcattc tcaggcgaat tgattctgac tccgtcaatt gctctgagcc ttgaaaccat
     6361 atattctctc agctcggttg catatttgag gctctgcttg cagtcgggaa tagccgccgc
     6421 agccgcaccg aagccggcaa ttgccggaac gggctctgtg ccagggcgga tcttgctttc
     6481 ctgcaatcct ccgtaggtgt gttgaacaag tcttgtgcct ttcttaacaa agagcgctcc
     6541 ggcacccttg ggaccgtgta ttttgtgaga gcttatggac ataaggtcaa cacccatgtt
     6601 atatggcttt agctgaacct tgccgaatgc ctgaaccgca tcgcagtgaa caagagcatt
     6661 tgcgcccgag tgagttactg cgcgttttgc aaattcgaca ggctgaatcg aaccgatctc
     6721 gttgttgacg tacatcatgc tgatgagcac aacggaggga ttggttgcgg cataaagctg
     6781 cctttcgtca attacgcctc tttcgtttac acgcagccga acaacgtcat agccctgatt
     6841 ttcaagagcc ttgacgcttt cctcaatgct tgaatgttca attgaggttg taattatacg
     6901 gctgccacgt cttttttgcg catttgccgc accgaaaacg gcaatattgt tgctttcggt
     6961 tccgccggaa gtaaaatata tctcgcttga ttcgcaggag agcgcttttg ctatattcct
     7021 ccttgaatcc tcgataagct cggatgcggc tatacctttg gcatggagcg acgaaggatt
     7081 gccccaacag gttttcagcg catagttcat tttatcgact gcctctttgc agggctgcgt
     7141 cgttgcgctg ttatccaaat aagcttccat agtccatacc tccgggcaac attaaaccat
     7201 ttttaacttt tatattatac atcatcattc atctactttc aatattttta ttacattaaa
     7261 aaatgaattt tttcttaatt ttaataaata tatggtggct gttaaacgtc ctatactaca
     7321 tcttatgcac ggcttttcaa taagataatg caaaatataa atgtttttgt tgcattttcg
     7381 ggaaatctat gataatataa attgctaaaa gaatgaataa atgttgaaaa aataacgtta
     7441 tttctgattt ctaattataa ggaataattt aattgagagg aaaaaagcgg tactatgagt
     7501 gaaaataaaa acatgaaagt gcaggaaaag tcgagtgatg ttgctgcttt cagcattatc
     7561 aacatggcaa tcagaaatct gcactatatt cttatttccg gcttggcggt tgcgatactt
     7621 gtttttttga tcgttacgat atttgtcagc cctacctatg aggttcgcac aagcttctat
     7681 gtttataata catcggcgag tgaatccggg tcggtcaaca gcagtgatat gcaggcggcg
     7741 aagaatcttg cgacaaccta cgctaaaatt ctcggaagca atgcggttgt ggatactgtt
     7801 attaacgacc ttgacgatat caacggcaat gaaagaacag ccctcagaaa caatattcgc
     7861 gttaacggtg ttaacgacac tcaggttatt gaagttaagg ttttgaccga cagccgcgat
     7921 ttgtcatatc agattgcgga ttccttttcg agaattgcgc ctaaagaggt tattcgtatt
     7981 gcaaaggttg gtaatatcga ggttcttgat cagcccgaaa tgccgaataa gaaatcctca
     8041 ccgagaacgc tttatgatac aatgctcggt tttcttgccg gaatgatact cgcattcgga
     8101 attatctgtc tcagagtagt ttccgatacg accgttaaaa attcttatga tgttgataaa
     8161 tcggtcggac ttccggttct cggcatgatt ccggaaataa aaaattcgac aaacaataag
     8221 ccttggacgt taaaagatgg gggagtggtc agctatgatg aagcagaagc atgataagca
     8281 gagctctcag gacgggaact ttctccttaa tttgcgctcg ccggcggaaa taaaggaagc
     8341 ctatgtaaaa ttgagaacta atttgatgtt ttgcatgacg gctgacggaa agcgaaattg
     8401 caaggttttt gccgtaacgg gagccaatca aggcgagggt aaaagcatta ccgcagccaa
     8461 tatcgccata agctttgcca tgatcggcaa gaaaacgctt cttcttgacg ccgatatgag
     8521 aagctcatct caaagacgta tatggaagaa cagaacccta acgggtcttt ccgattttct
     8581 cgccgagata aagccccttg aggttttttc ggtaaaagag cttcctttgt cgattgtttt
     8641 cacgggaacg cttccgccga atccgtcgga gcttctttca agcgaaaaaa tgtccggttt
     8701 tgttaaggaa tgttccaacc gttttgatta tattatcatt gatacgccgt ctttgaacga
     8761 ctacgcagat gcacagataa tttcaactct tgccgacgga gttattcttg ttgcaagatc
     8821 gggtgaaacg gattccgccg accttgctga atcgttagac tccgtaaagc gatcgggagg
     8881 aaatgtatgc ggcgtggctt tgaattttct taagccagac agatcaccgt ttttggcttt
     8941 gaaaaatttt aaaatctcat tgagataaaa attattgaat atattaaaag tacagtcttt
     9001 ataggctgta tttttttttg catttaagag cattttttaa aatattcgga tgttaaaacg
     9061 caatttaaaa aataactgta aaaatgctct gaaaatgttt ttaaactgct taaatatttt
     9121 atttgtttat atttagcaaa aaggtcatta ttatatcagt gatattgacg tttcctccaa
     9181 aaatattaca aaaatgaatt taatgcttga ataataatta tttttgtgat aatatttaac
     9241 taataataaa taattttttt attatgttgg tgaattttat ctaaatttaa ctaattatat
     9301 tttaattaca caaaatttta agaatatttt aaattggagg taataaaaaa tgtggttttt
     9361 gtgcttatgg caacgacatt ttttatcttt gacccgagca ttttgctccc gaagtcaaag
     9421 gccgttgtcg atgtcaagaa gttggtcaac accgttgaac cgactgtaaa gttctatgtt
     9481 ccggaggcga tttaccttaa tccggtggtt ggtacaggct ccaagccgta ttctttccag
     9541 tatttcatcg acagcgatga gaacggaacg cttcgcagaa gcgcaacgca gacaaccggt
     9601 acggtttatt tctcatgctc tgcggcttgt acatctatta ctannnnnnn nnnattgggg
     9661 cgatagcgat tctgtgtctc ctaatgtgac ggaaaacacc cacacaaatc agatagtaaa
     9721 gcaaacgatc acaggtggta ctacgtccgt aagggatggc agagtcaagg tcacggcaac
     9781 ctacgttgtc gagggcaagc catactatgc ctatgcttat acatatatgt attatcctga
     9841 gcttgatctc ttgacaggta cggcttcttc gtatgtttat aaggcgagcg gagcagagcc
     9901 gaagcttgcc gcattctcgt ttattaccgg tgtgcataag gtcggtaatg aaacaaccta
     9961 tggcaacaca aatgagcgtg gcgtcagtaa ctattacgac cagtacaacg gaaattcatc
    10021 aaagggctac tacggtattt ctccgttaat tccgggctgg gcacatgttt ggcaaaacgg
    10081 aagtcttgtt ccaggttctt ccggtgcgat ttttaaaacc gcaaacggcg atgcttatat
    10141 taatgaccgt aatgttgttt acggcgagac aacatcaagt cttccgaacg gttttcttga
    10201 cagaggtaac ggcggtgtat atttccgtga gagggaacgt ggcagcggaa ctaacaaaac
    10261 ctatggcgga ataagcaatt cctggggtgt tttgtacgtt gacacgagcc gtgtaaccga
    10321 ctacaatcag ataccgaatc ttcgcggcgg ttttatctgc cactattatc atcgctgcgg
    10381 caagcgtggc gagcttgaaa ccttcacctc aacggataat actattacga ttcttaacaa
    10441 cgttaatttc cgtaacggtg acggacacag ctatggtatg agcgacgcca accgattgag
    10501 cggcacagta agctccttgg acaaaaccta tactatgcgt gccaatttca agtatagaat
    10561 ggatgccgat atcggcagct cttcgaccgt aacaatgaac caggatttcg gtcttaaggt
    10621 ataccctgtc aataaaaaga gccttcgtga agcttacaga gcggctatcg agaacggctg
    10681 gcagtctgaa actgcaaaaa aacacggttg gtcatcttct gctcagtctc agtgggcgac
    10741 tgcctacggt aattttgatg cagcacttgc aaaagcgggt gaggttctcg gcagacccta
    10801 tgcaacttta agcgaggttt ccacagctct ttcaagtctt aataaccgag ttacagcatg
    10861 taacaatatt gttaatgcta atttccaagg cggagttaat accgacatgg aagctccgtc
    10921 atacactttc tatgttcccg aaacaattta tctagctccg tcaacaagcg caacaaaaac
    10981 gattttgtgc tgtgnnnnnn nnnngtacta tgttgaccgt gaaaactcag ttaacagctc
    11041 gctgagaaaa ggcgagaaca caagcggtaa catttacttt aaatgtgata aggcgtcttc
    11101 aatcaaaagt cttaccctga gtgactgctc atcagttaca ataggagcaa ctatttcgtc
    11161 aagcggaacg ctttctactg ccgttagtgc aggttcgctt tccgcaggtc tcaatccggg
    11221 agaaaaaaag cttcttacgt ggactattgt ctatgttgcg gacggcgttg aatacacagc
    11281 aaatgcatat actgtcgctt atgcaccctg gccgacggtt gtttccgctt cttcacaggg
    11341 acgaaacaaa cactccggca ataaaagatc atatattatg cagacaacct ggatttcggg
    11401 tattcattcg gttattgata acggaagcgg ttctgatgga agcggtgttt ataaatacag
    11461 ccctatcatt tcgtcatcat ctattccaac aggtgctgat agtaatatgg gtaatctgtt
    11521 taacgagggc acaggaggaa gcggattttg gaaatcaaac gagaatgcaa catgttacgg
    11581 agnnnnnnnn nntgctctcc tcgttgatac tagccgatac tcatattatt atcaagttcc
    11641 caatttgaaa gtcggtttgg attttaacat tgttcatggt aaccatgatt caaatacaac
    11701 ggatcttggc atctttaccg aaggatcgac tgaacaaaca acaaatttag gttctacgca
    11761 aacaggtaat gaccagaatt catatcctcg ccaaatatat aatttgaatt taggtagtcg
    11821 atacataggt tcaacaaatg aaaaccttgt tgttcgagga aaaacaaggt tcagaaacag
    11881 cggtggtggt tcttcacatg aaagtgtatc gtatgtttat cttaatgtaa caaaggttaa
    11941 caagtcttct ctcagaaact catataatac tatattgtca aagggctacc agaagtcatg
    12001 gtacacctcc gatacatggg acaaatacga aaaaaagctc aaggaagtcg ccttgcacct
    12061 cggtgacccg acatctaagg annnnnnnnn nggatacatc gggtattacg gatcttcagg
    12121 gattccagga cggacttgtc cgtaactcaa acggcaatac gacagccgtt catcttcgtg
    12181 acggttcttc tgcggactat aacggaaaaa caggttcggc aattatcggt acaacaaccg
    12241 agtctatgag ctacacctac ggagataccg tatacggtta ttataatgaa attcccggct
    12301 ttacggcaag cagatactcc gtaacttacg gctcaacaac aaagacgagc ggcgttccgc
    12361 aggacagcac acacaataat tactattata ttaagaacgc tgcgacctca aatgttgact
    12421 ggaaattctg gtatacaccc aatacataca gcataaatta tgaccccaac ggcggtacat
    12481 ataacggatc aacgggcttg acctcgtcct ctgttctgtt ccagtcaaag tacaccgtcg
    12541 gaaagatagg cagtgcaacc cctgccaatc cgacacgtcc gggctgtacg ttcatgggat
    12601 ggaagtgcag tgcggatgat atggtatatc agcgtggtgc aaatatcaac tgggaattcc
    12661 tcgagaatgt aacatttacg gctcagtggg aatacaactc gataaacctt cgctttaacg
    12721 agaatcatga cagtcttgcc gnnnnnnnnn nctcacacat gattcaacag cgtccgactc
    12781 aacggcttgc agctttactc ttgaaaagct tgacgacggt acgctcaaag ctaacggcaa
    12841 gcttgccgga gcgtatacaa tcggttttgt tccgatgaag ttcgaagcgg gaaagaccta
    12901 tcacttcagt aacctcgtca caggcggcag catgacaaac ggctgtttgg ttatggagct
    12961 tgccaaagag gacacaaata acataaaccc gagaaagttc tttaatattg aatcacattc
    13021 gactgcaacc ggaactgttg aaagcagaac cgccggggca gatggtggag aaacnnnnnn
    13081 nnnnccgata taacaatcac tcaggaaatg gctgatcaaa ttgccggtat tcgcttctgg
    13141 atctggcatg gctataatga gtacatgacg tttaataatt tcacgtttaa accacgtata
    13201 gagttgaaga gtgacagcac gactgtggcg acaagcagta cggaatataa caacatcttt
    13261 gcggattata ttcagtataa cactcttatt caggagcttc cgactgccac ccgtacagga
    13321 tataccttcg acggctggta cactcaggcg aatggcggca caaaggtaaa ggtaggcgac
    13381 cgtcttctct acagcgactg gaatctcaac aacaccagtc agaccctcta tgctcattgg
    13441 actatcaata aatataaact catgtatgag aatgagtttg actttgataa tttcaaattc
    13501 agtatggcta atcctcctgt gactaacaca aattcgccaa ttacaataaa tgagcatgaa
    13561 aattcaattc agttcacatc acaaaagaga gccgacggag taaccgatga ctttactccg
    13621 actggtcaga attatactcc tgccgaaacc ggctacatga ctcttgttcc gggtcatact
    13681 tatgagattt catatagcta taccgttgat aaggcggcga gcctgaactg ctatatattc
    13741 tcctatacat caacttcatc aacttcatgg agcacggagt acggaaatag cggcacaggt
    13801 acatattata acgtcggcgt tgctccgtcg ggagcgacaa ccggcactat gacgggatat
    13861 tataccgttc cgcaaggcag gccttatgca agactgcgtt tcggttcaac taccgaaggg
    13921 gtaactgtaa agttttctga tatctgcgtc cgtgatctca ccgacccgat ggattacaga
    13981 ggtaaatcag gcggcgacgg caaggaaaat tctctcaata agaagtttga taccgttacc
    14041 tataaaacaa ctcagagtcc gctcgcaaca ataactcgcc agggctatac attcaacggc
    14101 tggtatgaga gcgaaaatac gtcaaacggc aacggctacg gcactcagtt tactacctca
    14161 accgcaatgc cggcaaggga cgttcctctc tatgctcagt ggaaattgaa cgaatatcct
    14221 atcgtaatca atctcaatgc cgacagcaca gataaggcga caatcggttc atttacctac
    14281 agcaaggcaa acaacaatac ttcaaacggc aatatttctc tttcaacagc cacatcttct
    14341 cagcaggaat cggccggaaa tattaccaca ataaatgttg cttacggcac atcgttcacc
    14401 ttgccgattc cgtcaagaac aggttataca tttaccggtt ggacggcaag cgcaacacct
    14461 gcctatgctt cgctgacgga caacggcaac acaagcgcat cggcataccg tgtcggtgcc
    14521 ggcggagtta cattgaccgc tcactggtca agaaattcct atcccgtaaa ggcttacgct
    14581 tacggtgata tctacaattc tgcgaccttt gcactcggca gaggcggcac tgttcagata
    14641 gcatctgacg ccgcaggtac atcggtcagc aaaaatattg cttatcgttc cacaacaact
    14701 cttaaggcaa ctgcctcaac aggctatgct ttcgtcggct ggtacacaga ttccgcactt
    14761 acaaatctct acagcacaag tgcaagcatt acaagcgaat ccgtattgac agacggcaga
    14821 acctactatg cgaagtttaa ggtacagtca tatacaatta ccgttaatcc cgacaatgca
    14881 acgacgggaa caaccatttc gggttggtat tcggcggcag gaactgcggc tccttcacag
    14941 acgataacga cgtcaaccga aatcacaatg gtttacggct caacggtaac aatgaccgcc
    15001 ccgcagaaga cgggatatac cttcggcgga tgggaaaaga taacaggagc cggaacactt
    15061 acatcgtcta cgggcacttc aaccttcaaa tgcggcgcgg gtgatgcaac gatcaaagcc
    15121 cagtggacgg cgaatgtcta tacaattacg ttttt
//
LOCUS       DAWBSW010000026        15142 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_860_length_15142_cov_2.412010, whole genome shotgun sequence.
ACCESSION   DAWBSW010000026 DAWBSW010000000
VERSION     DAWBSW010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 15142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15142
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_860_length_15142_cov_2.412010"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6436..6445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8904..8913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11026..11035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12780..12789
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4241 a         3227 c         3711 g         3923 t
ORIGIN      
        1 gatatcatta tttccgacag atacgctacc tccaatgcct atcatcaggc gactaagatt
       61 gaaaaaagcg aaagagcgga gttctttaaa tggctggagg attttgaata cggacttatc
      121 ggtgtgccga agcccgatgc ggtaatttat cttgatatgc cgattgagat ttcgcagaag
      181 atgatgtccg agagatatca cggcgatgag acaaaaaagg atattcacga gagcaatctc
      241 gattacttat ataaatgcag agatgcggca ctcgacgccg cagaaagaat gggctggtat
      301 gtggttaaat gcaacgacgg tgacagcccg agaagtattg agtccatagg agacgaaata
      361 tactctatta tttccacgga ggttttatga gttattcatt tcacaagccc ggctttgatg
      421 acattaaatg gattaaagaa aaaatttcgg aaagaagaac ggagtgctgc gatttcacac
      481 ccggcaacct tataggctgg agcagatttt tcggcggaga aatcggcagt gtttcggatt
      541 gccttgttgt aaaaattaaa aaatacaatt catattcgtt tccgaagggt aaaaaccgcg
      601 aagaagcttt gcgcgaaatg gttccttatt ttgattatcc taaattttcc acgattgaaa
      661 agcatgagac ggaaattctc gaaaagcttt tcccgggaaa atatgagttc actgaggaac
      721 gcgacagctt tgactatgtt tattcccgtg agaagctcgc aacgctcggc ggcaagaaat
      781 atcacggtaa gcggaatcac atagcctatt ttgaaagaac gtttaactgg agctttgagg
      841 agcttaacag aagcaatatt gacgactgta tcgccatgaa tgagaaatgg tatactctca
      901 atgtcgaaaa ggacagggag ggaatagagg acgagcgtga ggttttgcgc cttaattttg
      961 cccattttga agaaatcggc ggcatcggcg gtgttctcag aatcgacgga gaggttgttg
     1021 cgtttacctt cggcgaaagg ctgaacgaca atacctttga tactcatttt gaaaaggcat
     1081 attccgatat caggggagcg tatccgatga taaatcagct ttttgcgcag aatctgcttt
     1141 cggattatga gttcataaac cgtgaggacg acgtcggctc ggagggtctg agaagcgcta
     1201 agctttcgta tcatcccgaa ttattggtta aaaaatattc ggctgttgca aaatgaaagg
     1261 tttgtcgcta tcatggttaa aattgttgat attaaaatat acagggagaa tataattgca
     1321 ttgtggcatg agtgcttcgg tgacgaaagg ggttatattg aattcttttt ggataactgc
     1381 ccgaataagc tttgtatcgg cagctttgaa aacggtgtgc tcgcttcaat gctttttctt
     1441 ttgaacggcg gcgtcggcgg actttcgtgc aggtatattt atgccgcctg tacgtctcga
     1501 aggtttcgtt ccaagggact tatgggcgga cttatcgaat actcaaagga atattgtgcc
     1561 gagaataaaa ctgacatgat cttccttgtt cccgccgagg agagccttta cggttactat
     1621 gcaaaattcg gatttattcc gaaaatgcag agagccgaat ttgctttgaa gggcacctcg
     1681 ggagaattta aagcaacgaa aacaacggac ataaaatcag tggcaaaaag acgattagag
     1741 cttctttcgt caatgaaatg ctttgccttt gatatgcaaa cgacggaata taccgtggcg
     1801 gagtttttgc gcacaggagg cgaaatatac tcttgcgacg gcgaaaacgg ctttcttgcc
     1861 tttaccgtta cggatggtaa aagcaccgtt gtcaaggaga ttttgaccga ttttgacagt
     1921 aattttacaa ttattttgga tttatttaaa aatttaaggg cggaaaatgt ttacattcat
     1981 gctcctttag tgtataataa tacagatatg gggttcaaag ctactaaatg tggtatgctt
     2041 tacccgatta ccgataattc aaaaagcttt atcaaggatg aagatatttt ctattccgga
     2101 atgtatcttg actgagaggt aataaaatga tttacgtttt tcttgcaaac ggatttgagg
     2161 agatcgaggg tcttacggtt gttgacttcc tgcgcagagc ggagcttgag gtttgcaccg
     2221 tcggcgtggg cggaaagctc atcacgggag cgcacaatat tccggttttc tgcgatcttg
     2281 acgaatccga ggtcagactt gacagcgagc ttgaggcagt cgttcttccg ggcggtatgc
     2341 cgggaacgct caatcttgag aagtctcaga ccgtgaaaaa taccgtgagc tactgtgccg
     2401 ataacgacag gtatgtttgt gccatttgcg ccgcaccgtc gatactcggt catatgggtc
     2461 tgctcaaggg caagacggcg tgctgtttcc cgggctttga aaacgagctt gagggcgcag
     2521 acgtaagcga gaattatgtc tgccgtgacg gaaaaactat tactgccaag ggtatgggag
     2581 ctgcgattga tttttcggaa aaaatcacag cctgtcttat aaacgagagt aaagcgaaga
     2641 agataaaaga atctcttcag tgtccctgtt gattgaagaa ggaggacgaa tatgaaagac
     2701 gagaacggca gagaatattc cgattacgat attgacaagc tcattgaaga gtattcgcat
     2761 atcaatcgga acgatgagcc ggagcaggct tcacagccgc caagggaaaa gaaaaagttc
     2821 gttgttcata tagatgaatc gctgattgac gattcgccgc agagcgagga acccaagccg
     2881 cagtccggcg gaatatattt ttcaaattat cccaaacacc gcaggtcacc cgatttgcag
     2941 gagaaacccg ctgccgagcc tgagcataag cacatggaga aaaaggctca caaggtaatg
     3001 cacggttccc gtgagaatgc ggtcaaaaaa gttaagaatg aaatcgaaaa agtcggcggt
     3061 aaggctgccg tcggatttct tgcgttcatt cttatttcaa ccatcctgct ttcatatgtc
     3121 ggaatcacct gtctcggaga tatgctcgca atcaacagga gcgacgagaa tgttacggta
     3181 gatatccctg ccgacgcaac ctacagccag ataattgata ttctcaagga taataagctt
     3241 atcaagcgca aggccttttg caaggtgttt acaaagttca gaggctttga cgacggaaca
     3301 tatctgagcg gtcagtatta tctcaacagc aagatgggcg ttgagggtat gctcaaggat
     3361 attatggcgg ctccggttac ggccgattcg atttcgctca gcttcccgga gggctggacg
     3421 gcagctcaga tcgttgaaaa gcttgagaaa tacgatgttt gcaactccgc aaaggttctc
     3481 acggcgatga aaaccggcaa atataattat gactttataa atgaaattac ggataacaag
     3541 agcagatatc tgaagcttga gggctatctt ttcccggata cctacgattt ctatgtggat
     3601 gcggatccga actatgttat cacaaagttc ctcgacaact tcaattccaa gtgggaggac
     3661 gagtacgata agcgtgccgc cgagcttgga ctttcaagag acgagattat aacaatagct
     3721 tccattattc agaaggaagc cgcaaacacg gatcagatga aggttatttc ttcggtaatc
     3781 cataaccgtc ttaaccatca ggcggactac ccgacgctcg gctgtgacag tacggctcta
     3841 tacatcagca attatgtaac tccgactgtc ggcgaggctc agggcgcaat ctactacagc
     3901 gcttacgata catcggcggt caagggactt cctcccggac cgatctgtaa ccccggaata
     3961 gacgctataa gagcggcgct ttatccctcg gataccgatt actatttctt cgctcacgat
     4021 aaggcgagca atatctacac cgcttcgacc ttcaaggagc acaagaacaa ccttgttctt
     4081 atcatcaagg cgaacagctc aactgattaa gaggatttat gagtaatatt aaaacagctt
     4141 ttcccgagct tctctctccg gcaggggata cagaaagatt tcagcgtgcg ctttatttcg
     4201 gcgcagacgc aatctatgtc ggcggttctg cgttttcaat gcgagccgca cctgccaatt
     4261 ttaccgcaga ggagctcaag gcggcggtcg atgccgcaca taaaaagggc gttaaggtct
     4321 atcagacctg caacaccgtg ccgagaaacg atgaaattga cagacttccc gattttctta
     4381 tggagtcggc agagacagga gttgacgctt tcatcatttc cgacctcggc gttatgacgc
     4441 tcgcaaaaaa atacacgccg aatgttgata tccatatttc gacccaggcc ggaattacga
     4501 attatgcctc ggcgaacgaa tggtataaca tgggcgcaac aagggttgtc acggcgagag
     4561 agctttccct tgaggacatc gcaacgctca gagcaaagac cccgaaggag cttgagatag
     4621 agtgcttcgt tcacggcgca atgtgcgttt cgttctccgg aagatgcctt ttgtcgaact
     4681 atcttgtcaa ccgtgattcc aacaggggag agtgcgctca gccatgccgc tggcagtaca
     4741 gcctgatgga aaagacccgt gagggtgaat atttcccgat cgacgaggac gagaacggca
     4801 cgtatatatt taattccaag gatatgtgca tgattgagca tatccccgaa atggtcaagg
     4861 ccggtatttc gagctttaaa atcgagggaa gagcaaaatc ggcatactat acctcggtta
     4921 taaccaacgc ataccgtcag gctatcgacg gatatctaaa aaatccgacc gatgattaca
     4981 agcctgaaaa atgggtcgta gacgagatgt ataaaatcag cagccgcgaa tactgcacgg
     5041 gcttttactt cgggcacccg agggacaacg cgcagatcta ttacgacggc ggctacaaga
     5101 gggactggag cgttatggcg gaggttcttt attccgagaa cggacgtgtt ttctgccgcc
     5161 agaggaacag attttttgaa ggtgaagttc ttgaggtgct tcagcgggga gtcgaaccct
     5221 acgaggttac ggtcaccgac ctgagagacg aaaacggaga gaaaatagat aaagctcccc
     5281 atccgatttg aatgtgaaaa ggatattccg ccggacgccc ttttgagaaa ggctgtctga
     5341 caggtctttc ggaggcttat atgcttttaa gtgatattct caacggtata gattattctt
     5401 gcgataattt taagaatact gatattgttg acattacata caattcaaaa aatgcaggcg
     5461 agggcaagtg ctttttctgc ctttcgggaa cggttaccga tgggcacaaa tttgctcgca
     5521 gtgcctacga cggcggctgc agatgtattt ttgccgagca cacggtagaa cttcccgagg
     5581 actgcgttca gataatcacc gacgatacgc ggcacgctct tgcaattgct tcggcaaact
     5641 ttttcggcag accctgcgaa aagctcaagg tcgtcggaat aacgggcaca aagggtaaaa
     5701 cgacaacggc acatattgtg aaagccttga ttgaagcctc gggcgagatg tgcggaataa
     5761 tcggaacgaa cggagccgca tacggcaacg cttccgagga aacgaagaac acaacccccg
     5821 aaagctacga gcttcaaaga ctttttcgca aaatggttga cgcaggctgc aaatactgcg
     5881 ttattgaagc ctcgtcactc ggactcaaaa tgcacagaac cgacgggatt ccgtttgagg
     5941 tcggcgtttt tatgaatctt gctcccgacc atatcggcac aatcgagcat ccgacatttg
     6001 aggattataa aaacagcaaa aagctccttt tcaagatgtg caggcatgcc gttgtaaatg
     6061 ttgatgaccc ggcatacacg gatatgcttg aaggctcatc ggcagacgtt acaagcttcg
     6121 gactcggtga tgccgacgtc agggcggaga atgttgagct tctgagaagc aaggatattc
     6181 tcggcgtttc ctttgactgc attgacggag aaggaaggtt ttctattgag gcatcaatgc
     6241 cgggatattt caacgtttat aatgttcttg ccgcagcggc tgtttgcggg gtgctcggct
     6301 ttgaggttaa aaaacataaa gatgttttcc gcagcttcag cgttgcagga aggtttgagc
     6361 cggttcacgt cagcgatgat tttgacgtta ttatcgactt tgcccacaac ggcttgagta
     6421 cacacaacat tatttnnnnn nnnnnacttg ttgcaggcag ggaagccgac ctttcaattc
     6481 tgacaacgga ggaccccggc agggaggatc ccgtgaaaat tgccgaggag atcgcttcct
     6541 atatccgtca gacaggcgga aaatatatga taatccgtga caggagagag gctatagttt
     6601 atgcgctctc ccatatgcaa aaaggcgata tacttgttct cgcaggaaaa ggaaacgaaa
     6661 cctttataaa aataaacgga ggaaagattc cctattccga gcgtgaggaa gttgagaaat
     6721 atatcaaatc aaagaagaat taaactctgg ttaaagaaga attaaattct gttttcagtg
     6781 aaaattcaag gaggtagtta tatgccgcag aaaggaacgg gcgcagcaaa aaagaagaaa
     6841 agaaagagac tgaacgattt atctaatgca ggcgctttct ttatctcgtt gctcgctata
     6901 ttctgtttta cactcgtgat atgcttgggc tatgttttta tcaacgcagt cacggttgtg
     6961 gcaggcgatc ccgttatcaa tcttgagagt gcaaggaaca atcagaacca gacctcgttc
     7021 ctctatgcca aggactccga cggaaagcag atcgaggttt tgcgtttgca cggtgaggaa
     7081 aaccgtatct gggtcgattt cgacaagatt cccaagcaga tgaaggacgc tttcattgcc
     7141 cttgaggata agagattcaa taaccaccac ggtgttgact ggattcgtac catcggcgtt
     7201 ttcgttaagc agaacgacca gggcggttcg acgataactc agcagcttgt caagaacatt
     7261 acgggcaaca ctcaggtaac ctacgtccgt aaatataacg agattctttc tgcgctgaac
     7321 cttgaaaaga attttaacaa ggatcagata ctggaggctt atctcaacac gatctatctc
     7381 ggtcagggct gttacggcgt tgagaccgca gccgaaacat atttcggaaa gtctgtcgat
     7441 gagcttaacg ttgccgaatg tgccgttata gcttcgatta ctcagatgcc gtattcctat
     7501 gacccgatca acaatcccga gaataacagg aagagacaaa tctactgcct tgatgaaatg
     7561 ctcgaccagg agaagattac caagaaggaa tatgacgaag ccgttaacta tcaccttatc
     7621 tatacaacgg atgatgatta tgtccccagc gaggcggagc ttgagcgacg caaaaatgag
     7681 gagaacgcaa aggcttcatc ggagttccag agcttctatg tcgattatgt tatcgacgac
     7741 gttatcgcca ggcttcagag cgatctcggc tattccgcaa atgaagcccg tgacgccatc
     7801 tacggcggcg gtttgagaat ttacacagcc gttgatcttg atgttcagaa aacggcggag
     7861 gatatttatg taaatcgaaa gggctttgag gataggaaaa acgcacagtc ggctatcacg
     7921 attatggact acaagggcag agttgttgca attgtcggtc aggcaggcgt taaggagggc
     7981 aaccgagttc tcaacagagc aagctcgtct ccgagatctc ccggttcttc aatcaagccg
     8041 ctcacctcat acagtccggc aattgagatg aacgagatca catggtcgtc ctatctcctt
     8101 gattccgctg tcagctacgg cggatggaga acacgaaatg ccgacggcgg ctacggctcg
     8161 ggctataatg ttactgttca gaacgctctt gcaagatcac tcaacacgat tccggcaaga
     8221 atcgttatca atatgatagg cataaacgag tcctataact accttgtaaa taaatatcat
     8281 atcacaaccg ctgttgaggc gtcggataag agcgcggctc cgctttccat cggctcaatg
     8341 acctacggaa caacatcgct tgaaatgact gcggcttatg ctgctttcgg caacggcggt
     8401 tactattaca agccctactc tttctactat attgaggatg ccaacggcaa ggttcttata
     8461 gacaacagga acaataaggg cgagcagatt atttcaacgg gaacagccga ggttatgaga
     8521 catcttctta tgaccgttac aaccagctcc tacggtacag gctcaagcta caaggtaagc
     8581 ggtttcgata cctttgcaaa aaccggtacg acagacgaca acaacgataa gtggttcgtc
     8641 ggcggaacgc cttattatgt tgctgcggtt tggtacggct atgatattcc cgaatatatc
     8701 agcacatggg gtaaccctgc cggtgctgtt tacaagaccg tgtttgacgc aatacatgag
     8761 gatcttcccg ataaggattt tgaggatctt gacggtgcgg ttcagaagga atactgtacc
     8821 gatacgggta agcttgcttc ctcaagctgc tattcaacgg ccacaggctg gtacaagaag
     8881 gacaatgttc ccgagtattg cacnnnnnnn nnnctactat tcatcaaacg atagcggcga
     8941 cgacaaggaa gaagaaaaat caacaaaatc aaatcagccc gccacctccg cagctcctgc
     9001 gacagaaagc aacggcggcg agcaatagaa tttttaaaaa agtcaagcaa taaataataa
     9061 cacggaggcg gttgcatggt tattatgtct gtcgattacg gtgatgcgag gacgggcata
     9121 gcgctttgcg accgccttga aagtcttgcg catcccgtgg aggtcatcaa gcaaggctat
     9181 gagccgaagg tgcttgagag aatatcggag cttgcaaaaa ttcacaagcc ggagcttatt
     9241 gttgtcggct ttccgaagaa catggactct tcggcaggcg agagggcgca gaaatgtgcc
     9301 gaatttgcaa agagaatttc cgaggcaacg ggcattgaaa cagatctttg ggacgagaga
     9361 ctgacgaccg tttccgctca tatgtatctc agcaacaata acgtcagagg aaagaagcgt
     9421 aaaaacgttg ttgatgctgt tgccgctgtt attattttgc aggactatct tgattacagg
     9481 agaaataaaa atgcaagcaa gggcagaaaa tataattaaa accttttttt cggggctata
     9541 ttgcgttgtt tttatgctca tctcggcata tgcaggtatg catttcacgg gcagactcgc
     9601 atttcagatt atattgacgt tttcgctcac gcttattttt acatttgttg ccgatatttt
     9661 aaaaataaaa atgcaggtta tctcttttgc ggcttgcttt tctgcggcat tttgggtcat
     9721 gtgcgcaatt gtcgctcaga tgcttttgaa aggcactttt ttaattttca attcggcatg
     9781 ggaatatatg ttttattacg acagcatcgg catggttttt attgtgctga tagttgtcac
     9841 ggcatatcag cgaataaaag catttataaa aaaagacgca gaaataaccg agcagtatga
     9901 taaattctat tcggctgttt ctgttgcttt tactttattc tatattgttg tgctggttta
     9961 ctgcttcttc atttgcagga ttcccggagg agagcgtgtt gagccaaacc tcgatccgct
    10021 cgatgtgttc agagtaacgt ttttttcggg atcgcttgat tatgaacggc taattctttt
    10081 cctgggaaat attgcgattt ttgttccgct cggctatctc ctttgccaca ggctgaacag
    10141 gggagggctg aaaaccgttc ttgtcctcct gccgtttgtt ctcagcacgg cgatagaggt
    10201 ttcgcagtat tatttcggta tgggtcaccc cgatattgat gacgtgattt tgaatgttat
    10261 cggattctat atcggcgtat tgctgaagct tttgtttgat aaagttttta cgctgagacg
    10321 atgattgcag gcagagattt aagcttaatt tatctgacct tgggtcgatg tgggcatcga
    10381 cccctacgga ttatgctcca accgtacctc tgcgtaacaa tattttttcg caccatacaa
    10441 atttttcgca cattacatat atttattttt tcttcttcgg caaaagcaga tcctttgcgc
    10501 cgacgagaat gagaataacg gcgaaaatta ttctcagcgt tctgtcctca aggcggttga
    10561 gaaggaaaaa tccgagcgca acgccgagga gaccgtagcc gatgtaggtc aatgccgtct
    10621 ttttatcaat cagatcgttc tttatgtgaa atataattgc caatatggcg gaggggataa
    10681 aaaatataag gttaattccc tgcgaaaccg cctgcggcat atccttataa agcgtgaggt
    10741 agagaataag gattcctcct ccgccgaagc ccattgcgcc gagaattccg gagactgttc
    10801 cggcaataat atcaagtatc atttcatcag catcctgatt cctgcgtaga ttataaaagc
    10861 cgaaaacacc ttgctcaaca cgtccttggg tatcttcggg agtaggagtg aacctatcgc
    10921 cgagccgata agaccgggga taagataaac atagctgtcg ctcagcctga cctgaccctt
    10981 tatcaggtag ccggcggcgc ttatcacagt cagcggcaga attatnnnnn nnnnngaatt
    11041 atcgctattg ccgttgcgtg cgattttgtc tgattcgttc cgcggagttt cagtgcttca
    11101 acagcaacaa ttcctccgca tgagccgatt agaatattca cgacgcccgt cagaatcccg
    11161 agcagaggag tcattatttt tttcatagtt cattcaccgt ttgcatattt tttatgtatg
    11221 aggtggtaac gtgaaaatcg gaaaaaagag tatcatcgca attataactg cgtttcttat
    11281 aatattatcc gcaatgcttt atcttacatt catggctaat ttttccgccg ccgaggcttc
    11341 ttcaatgccg attacgcaga aaacggtaat cgttgacgca gggcacggcg gcgacgacgg
    11401 cggagcgata ggaattgacg gaaccgttga aaaggacatc aatcttgata tcgcattgaa
    11461 gctcgaaaaa attttgaaat tttacggctt taatgttata atgacaagaa cgcaggacgt
    11521 catgacctgt gacgacggtc ttgattctct gagaaaaaga aaaatttcgg atattcacaa
    11581 ccgctttgaa ttgatgcgga aaaatcccga cgcgattttt ataagcgtgc accagaataa
    11641 atttgaggac tcctctcagc acggcacaca ggttttttat tcgggaaatg atgagagaag
    11701 caaggagctt gccgaagcga ttcagacctc ggtaaccttg actttgcagc ggaaaaatga
    11761 cagggtcgtt aaaaaatcag gctcgggaat ttatcttctt tatcatgcga agatccctgc
    11821 ggttctcgtt gagtgcggat ttatttccaa ttccgacgag gtgaagaagc ttaaggacga
    11881 aagctacaga atgaagcttg caattcttat tgcagacgga ctgttaaaat atctaagtaa
    11941 ccactgaggt gataacatgg ctcagaaagc caaaacttta tatgtttgct cagcctgcgg
    12001 acacgagagc gccaaatggt acggctgctg cccgggctgc ggcgagtgga acaccatgga
    12061 agaggttttt aaagcacctg aaaaaacaaa ggcctccggc agaacagcgt cggtcggttt
    12121 caattcaaac gttcagatga taaacgacat aagctgtgac ggcgaggttc gccataagac
    12181 cggacttgag gagcttgacc gagttctcgg cggcggaata gtcaaaggct cgcttgtgct
    12241 tctcagcggc gaccccggca tcggtaaatc gacgctcctt ttacagattt gccaatacct
    12301 cggcagaacg cttaaaattc tatatgtttc gggcgaggag tcggcgcatc agatcaagct
    12361 cagagcggac aggctcggag tcaatacgga aaatcttttt atcctctgcg aaacagacgt
    12421 tcaaatcgtt gcagagcata tgagagcgtc aaagcccgac cttgttatta ttgattcgat
    12481 tcagactatg aatgtttctg aactgaattc ctcgctcggc agtgtcgcac aggtcaggga
    12541 gtgcacaaac tgtcttatgc agacttcaaa aagcctgaat attccgacga tagttgtcgg
    12601 tcatgtcaat aaggacggca atatcgcggg tcctaaggtg cttgagcata tcgttgacgc
    12661 agtgctctat tttgagggcg aaagacatct ttcctaccga attttgcgtg ccgtcaagaa
    12721 cagatacggc tcaactaatg aaatcggcgt tttcgagatg aaggataacg gacccagcgn
    12781 nnnnnnnnng atgatttcgg gcagaccgaa gaacacttca ggctcgtgcg ttgcctgtct
    12841 tatggagggc tcgcgcccga ttcttgccga ggttcaggca cttgtcagtg cgtcgggcta
    12901 cggtaatccg agacgcatga cgaacggctt tgattataac aggcttgcga tgcttgccgc
    12961 cgttctcgag aaaagagcgg gatttttcct gagcaactgc gatatttata tcaatgttgt
    13021 cggcggactg aagctggacg agcctgcttc cgacctttcg gttgcgcttg cgcttatttc
    13081 gagtctcaag gatacgccca ttcgtgatga tgttattgca ttcggcgaga taggtcttgg
    13141 cggtgagata cgctcgatag gtagctgcga acagcgaatc aaggaggcgg caaggctcgg
    13201 ctttaatcgc tgcattgtgc cctaccataa ctataaaaac ctgaattcaa atattaaatc
    13261 gctcgttgaa attgttccgg caaggacaat aagagcggca tttgattcgg caatataaat
    13321 tatgcctctc cttattgcaa ggggaggcaa gctttttatg tgctaaagtt actttatcaa
    13381 ttttctttgg tgacagtggg catcgcacct tacgatttgc tatgacaatt acactttttg
    13441 taggggtcga tgaaacttcg tgtgcgccca agcatgagag gcaagtgcgg aaattgtaac
    13501 ttgatattca ctattgcact ctcaatacaa acaaatcaaa aaagcaagga agtgcgtgac
    13561 cgttccgccg aggatgaaaa ggtggaagat aaagtgataa tatcttttga atctgccgat
    13621 tgaatagaaa atcgtgccga gcgtataaat tgtgccgccg agaacgagca aaccgaaaag
    13681 aagaagtgca ttcgggtcgc atttgtaaat cggataaaag agaaaaatgc acatccagcc
    13741 gagcaccatg taaagcacca tttgaagctt tttatatttt tcctgatcta taaatgtcag
    13801 cactattccc agcagtgccg tcactacgct gaccgaaatg caggtgatag caagggcttt
    13861 gtaatagggg agcacatcga gtatcataag cggaatgctt gtgcccgtaa tcaaaacaaa
    13921 aataaccgaa tggtcaacaa gtcttgcaat tttctttgct tttcccgggg aaagtctatg
    13981 ataaatcgaa gaaccgctgt aaagaataat catagcaacg ccgaaaatta tcgagctgat
    14041 tatgtgactt atatcattcg atctcgtgag cagaacagcg gttgcagccg ctccgaaaac
    14101 aactccaaca aggtgcgata tcgagtttgc tttgtcctct ttttcagtat aataaggcag
    14161 gcttacattt tccaaaacca tgttgatttc tcctctgttt attttattct tttgagccgc
    14221 cgattaaatt tcatgcaaag acgttgcgag agggatgctg ttcaaccggc ggcggtttat
    14281 tgatttcatt gtacgacgaa aaccggcaaa agtcacccta caccgtgttt cgggctctct
    14341 acagtgattc tactccgggt agagtgattt ttttgatctc taccgagcga aattcgacgt
    14401 tttttgaaaa aaattcccta taactcttga taaatctttc acagactgat ataataatcg
    14461 ggtaaccact gatttattga gaagcacaaa tcttgacggt atatttcttg tcatatcgca
    14521 caaaccatct tgtcaatacg aacagtatta actgcgatgc cttgtacgat ctgacgaaaa
    14581 atctactcgc ctatttttgc gctttcaatc aatcagcggt tacccagatt gggatacacc
    14641 aaaaccggta aaaccccgaa aaaccgaaag gagattttta taatgaacta ttcagcagaa
    14701 gttcagggaa tgtgctgtgt agccaagggt ccgaaacatg atccggctcc gattcccgaa
    14761 gaaggcaagt ggatcgctgc aaagcagatc actgatattt caggctatac ccacggtgtg
    14821 ggctggtgcg cacctcagca gggtgcctgc aagctttcgc ttaacgttaa gggcggcgtt
    14881 atcgaagagg ctctcgttga gactatcggc tgctcaggta tgacacattc cgcagctatg
    14941 gcggctgaga tccttcccgg aaagacaatt cttgaggctc tcaacactga ccttgtctgc
    15001 gacgctatca atacagctat gcgtgagctt ttccttcaga ttgtttacgg tcgtacacag
    15061 tccgctttct cggaggacgg tcttgtaatc ggcgcaggta tggaggatct cggtaagggc
    15121 ttccgttcaa tggtcggcac aa
//
LOCUS       DAWBSW010000027        15035 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_869_length_15035_cov_2.588852, whole genome shotgun sequence.
ACCESSION   DAWBSW010000027 DAWBSW010000000
VERSION     DAWBSW010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15035)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_869_length_15035_cov_2.588852"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    582..591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4072 a         3478 c         2939 g         4536 t
ORIGIN      
        1 ttcgatcgtt ttatcggcat tttgatacat ttcttgacgc tcatcaaact cttataaaaa
       61 atatttttat tgacattatt attcctctat ggtaatatga agttgcagat agaaatatct
      121 gacattaaaa aggaggataa caaaatggaa gctattcttg cctttgtaca ggagctcctt
      181 actcatctta aggaatttga tgcagcagct attattgaaa ttatcaaaaa cttctttgca
      241 ggtcttgcat aagttaaacc acttgccccg tttttttcgg ggcatttgtt aactatttca
      301 tatttttttc tgttttatac attgttaatt acgtgttatt ttacctattt tacacattgt
      361 ataattgaca aatttatact ttatatggta aaatatactt gcagatagtt atctgacact
      421 aaaaaaggag gaaaaatcca tggatgctat tctcgctgca atccagaagg ttcttgaggc
      481 tcttaaggat attgacatca ctcctatcat cgaagctatc aaggagttct tcattaagtt
      541 tgcttaattg aacttacaaa tgaatcggcg tcctgcaaaa gnnnnnnnnn ntcttattat
      601 aaaatactct ctgtgggttt gatattcatt atgcttataa tttcggaaac ggttttttcc
      661 acgttgtcgg taacctcaac ccgtatatcg gcaaggaatt tataaatcgg agttctctca
      721 tcatcaaggc gtttgagcgc ttcaaaattt cttatcagcg gaacaccgtc aaatttcaat
      781 ccgctcagct cacgggtaag ccatacgaca tagccgttct gacggatata atactgattt
      841 tccttgctga gtatcgctcc gctttcgcac gcaatgacct gaccgccgtt gcgtgttatt
      901 tttttaagtg tctccgtctc aagttcacgg tagtatcttt caccgtatgc caagtaaacc
      961 gttgaaagag gaacccccgc tttcattgaa ataagccttt caacatcaat gcaaggtctg
     1021 ccgagcttag cggcaataac ctttgcaatc gtcgtttttc cgcagccggg aagtccgacg
     1081 agaataatat tctttctgcg gtcactcatc gtcttatacg ttgttctgat aagctcgtca
     1141 tcaggctcaa ttctgccaaa cttgatctcg gtctgatacg cctgagcaac aagcatggaa
     1201 agtccgtcgg aattgggaat atctctgtcc tcggcatcgc agataagacg ggttcttctc
     1261 gggttataga taagctccat gacgccgcaa agcctcggaa agcggtcaag ctcaataaat
     1321 ttcattccgt tattcggata cattccgttg gctgtggtat tgattattat atcaacctcg
     1381 tcgtgattgt aaaaatcctc cgactcctca atctcctgcc ttgaaacagt gacaatatcc
     1441 tttgccccga ggtcctttat aacagcctga actgtaagag aatcactgcc tgttccgaaa
     1501 acaatcacat ttttgcccga aacatcaatg ccacacttgt ttatcataaa gcaaaatccg
     1561 aaatattccg aattatcgcc tatgagtttg ccgttttcat caacaacgat cgtatcgacg
     1621 ctgccaattt tctttgccga ctcagttata acgtcgcagt atttcataac atctttctta
     1681 taagggaaag tcacattgat tccggcaaag tcacgctttt caaaaaaagc gtcaagctca
     1741 tcgggttcaa gctgaaaaag atcatacctg ttatttccca gctctctgtg aatttccgga
     1801 gaaagactgt ggagaagctt tcttcctaac aatccgtatt ttttcattat tcatgccctc
     1861 cgtctttatc atttttcctg ttttttattc ctgccgtaca acctgccgca accgcaacaa
     1921 gcgtcgccgc gacacccaat gccagcatca gtttctttct tctgttattt atctcaactt
     1981 cgttgcctat acctgaatct ataactgcct gagacatctc gtcttcgttt tcgctgtcat
     2041 tatctgcggc tttgccagtt cttgatttct ttggctttgt tgttttgcgc tttgtggttt
     2101 tgtgctttgt ggttttaatc tttgtggttc tcactttagc ggtcttgacc ttggcatctc
     2161 tctcgccgga atccattatt gcggcctcgt ctgcataacc gataggttct tcgtcctcag
     2221 tgatatcaac attgtatgta ttcgacttca ctccgtttgt atcgatcaca ttgacgttca
     2281 taatgacatt ctccggcact ccgctcttta taccgaccgt tgtctcaagc agaacgtttt
     2341 tgctgtgaac atcgtgaagc tcatcttcaa gctcggcgaa atagacattg ctgtcataat
     2401 ccgccgtacc gtcacacatc atctttatat tgcccatccc gttaaaattc attatcacgc
     2461 cgcaggattc aatgttgttg aattcattaa attcaaacgt aaacaagaga taaatacgat
     2521 ttatgcttcg gtcatatttc accttaaccg ctgcgaagca aagatcgctg tttgattctt
     2581 cgcccgcctc aagaaacgga caaagctcag ccttctccca aagcgcatta tccgagattg
     2641 caagggctgt tgacactgat gaacagagaa tggcagaaat aatcattaaa ataattatta
     2701 ttttaaaaga cctgcgcatc tccatccctc tttttcagtt tacttttatt gtaataattt
     2761 caaaaggttt tgccgtgaag cttacggaat tgttttcaat taccagcttt ttgatgtctc
     2821 tttcgagaag gtcgcaaaca gttgcttcat ttatgtcaaa gccaaagtga agcgttacat
     2881 ctgttctctt gttatagctt tcatagccgc ggacaataat atcattgcct ctttcggctt
     2941 tttttactgt ttcaacaatg aaattatcct tgtcgcatga aacaaacgaa tactcctcgg
     3001 gaagggtacc gtcccgtttc tctatctgga aagcctcaag cggattgttt atctcataag
     3061 caagctgaac cgtgcctgcc tccctgtaat tacccgcatg agggaacagg gaatatgtga
     3121 aaatatgctc ccctttgtct gcatcgggat cgggataggt cgcacactta aaaagcgaaa
     3181 gcttcattgt accgttgtca acattgtgac cgtacttgca gtcattgaga aggctcactc
     3241 cgtatccgta atcggaaata tccatgtact tatgtccgca gacctcaaac ctcgccgcat
     3301 cccagcttgt gtttttatgc gtcgggcgct caaccgagcc gaactgaatt tcgtaggttg
     3361 ccttatcgga attgatatca atatcaaaag ccgccttgac cataatgtgc tcctgatgcc
     3421 aatcggcatg ggtatcaaag tcgattcttg cgctgttgtc atagaaccaa atcttctggc
     3481 tgaaatccga cttgtagaac tttcttttaa tactgaagcc gaagcgtgca ccctcgtcga
     3541 taaattcaac cgaggaaaca tcgtttatct cgtacttctt ctccgtataa tagtttgaaa
     3601 gctcccatgc gtcatatgaa taaggaaaat cttcaaacgc ttcaatataa ttagctctgc
     3661 cgccctcacg aagcacttca cggtcattta tcttatcgta aagccttgtg attacatatt
     3721 cgtcgttgaa ttcaacgata taattcgagg tttcaagatg ctttccgtca agcttgtaag
     3781 atgacttgta ttcgtcaagc ttaaccacct tatatccctt ggcaggtatt ccgttcacac
     3841 ggtaggtctt tccctcgtag ttaagaaatc cgttatccgt gaatgaattt gaattgaaga
     3901 caactgttcc tccgtcggtt tttatatttg atattacggt attatatgca tcgctctccg
     3961 ccttgtgacc gatttcggca atttttgcat aatccttgtc gcattgctca taaacggaac
     4021 gaattgacga gccggggata atgtcatgga actggttaag aagaatacac tcccaacccc
     4081 cgttgagctt gtcctgcgga tagctcgcac cgaaaagctt atatgccata gatgaaagca
     4141 tctcggcttt ctgatagagg atctcgctct ttctgttgtt cctcttgttc ttcgcctgtg
     4201 aagtatatgt acctctgtgg aactcgagat aaagctcacc gacccatttc ggcagtctct
     4261 tgttaccctc ggtctttttt ctcagccttt cgaggaactc gcctgcgaac tcccagtgcg
     4321 gctgcggaca atttgcaacg ccgtatttaa gtctttcgag caattcaata ttctcggtct
     4381 ccggtccgcc gcctccgtca ccgtgaccga acggcatcat gacctcattt gtaagctcct
     4441 tcggctcgaa gcggtcatat gtgcccttga gataagatgc tcttgtcatc ggtatatatg
     4501 ttgcaaagat gccgtcgagt tcgcccttgt tattaagctc ctttgcggtc atgaaataag
     4561 aaaagacctc agagccgtca atgcccttcc acataaatgc gtcataaggc attctgtttg
     4621 tctcgttcca actgattttt gatgtaacga atttatcaat gccgctcttt ttcataatct
     4681 gcggcatggc tgcactgtaa ccgaaaacat cgggcagcca gattatcctg ttgtcaacgc
     4741 cgaactcgtc cttgaaaaag cgcttgccga aaatgatctg acgcacaaga ctttcaccgc
     4801 tcgtaagatt acagtcggct tcaagccaca tcgacccctc aagctcaatg cgtttttcct
     4861 tgacaagttt tttaatctcg tcatacattt cggggctttc ctctttaagg aatttcaaaa
     4921 gctgcggctg acttgacata aatttgtaat cgggatattt tttcatcatt tcgataacac
     4981 tgccaaaggt acgctgaacc ttttccctcg tctgagcgag agtccatcgc catgcaacat
     5041 cgatatgagt atgaccgatc acgctgattt ttatatcctg atcgtgacaa tattttccgt
     5101 aaaactccgc catgagatat tcgttcgccg catcaaccga acgcagaaac tcctccgaca
     5161 tcggagcgca ccaattgata taattgattg ccgtgtttaa atattctctt acgtcggcat
     5221 attcctttgt gctctcgtcc gtataataaa gaattctgta gggcgcttta agattataat
     5281 aaagcttttc aacctcacgg ttaaattcgc aaagctgagc aaaaaagtct gttctttttc
     5341 ccgacggctg agaataagcg taaacatgaa cgtcgtagcc gttcctgttg ctgtcaagct
     5401 taacatatct gtgttttgta tccattccgc gtataagctt gccgtcaaga taaaccataa
     5461 actgcggctc ccagtcctca tcatagacat cggtagaaga aacatacagc tcaagatcct
     5521 tgcttttgag ctcctgcgga atttcaatgt gtttgaaaaa ccatctgtgt tcctccggct
     5581 tcatgcccca gcgctccgtt tcaccgaatt cacgccactc gccctcatca ggcgacggga
     5641 caacattgcc gtttttatag ccgcactccg ttgtcagcca gccgtttatc ggaattctga
     5701 caggcgtgat atatttttca agctgcttta ttacttcgcc gattcttttg tcaagaaaat
     5761 tgttcatttg ccgctcctca gataagtcca tatatttttt ctatttcagc cgattttgag
     5821 aaatcggtga tttttaaatt ttcaattatt cttttttgaa tctctttgtc gttcaaaaat
     5881 ttcgtaaggc cctcggcaca ttccttgttt tcaagcggaa cgatcacacc gtcgaccccg
     5941 ttgtttatct gacttttcgc cgtagcatag ttcgttattg caacgagctt gccaagtatc
     6001 agcgcttcgt tgacagtgac agcattgccc tcatagcgcg aaggctgaat ataaaaatca
     6061 caagccttaa aataagggta cgggttcgtt tttttcccga gaaggataac cctgtcctgc
     6121 ataccaaact cggcgatttt tctcttaatt atttcttcgt ctgcaccgta gccgattata
     6181 taccacttga cattgttgag catcgaacag atttcgggca cgttgtcaaa attcttcgca
     6241 tcgcagtaac gtccgaccga aaggaatttt atatacccat catccggcat ttcgttcgtc
     6301 acgtcgaatt cattcgcctg tgtttttata aattctgccg ggtgaatatt ttcgataacc
     6361 tccaccctgt cggaaagcga cggaaaagtt tttacaaacg cttcttttcc gctctccgaa
     6421 acggcaatta ttttatcata tgccgaccac attttcagct ctgattcaac atcaatttca
     6481 atgaacgaat aatccgtatg aatccatgcg atttttttct ttgccctggt tttctccgct
     6541 gcaaaataat gcggcgtcaa aaaactgatc acaagatcgt aatccttttc ggatatcatc
     6601 ggcatatggc gcacggtgta tttgtggctg tattcaatga aaacatattc actgctttta
     6661 aagctgtgct catcggcaaa cttcgccgcc ctttttttgc cctcatatct gccgagagca
     6721 acaccgaatg cgcctttttt tataacgtcg gcaagcggaa cggcaagtga ggaatagctc
     6781 ttattttccg gcagaagctt gaccttttcg ggaatatatt tcatcagttc tccggtgtgg
     6841 cgcagaagaa aaagctcaat attatattta ttacagtcaa aagcatcgag aagaccgagc
     6901 aacgctcttt ctgctccgcc cagctccata caatggctga ctataagcac atttttcatg
     6961 ccctacctcc gatgccgatt tctaaaaaca taacccgatt atataaaaag tattatacta
     7021 atttaaaaag ataaagtcaa tgaatgattc gacatatttc aaattttttt gcgaattatg
     7081 ccgagtttca tttgcgaatt tgatatttct ttatttatat gctataattt tcagtacaga
     7141 taatcgaaga ttaaacaagg ggtgatttga atggacaata aaaaaatcga taagcttgcc
     7201 gaaatgataa acaattcttc caaaacggtt ttcttcggcg gagcaggcgt ctcaaccgaa
     7261 agcggagtta aggactaccg aagcaaggac ggtctttaca acaccgtgcg tgaatacggc
     7321 gtatcgccgg agacgatttt aagtcacagc ttctttattt caaaacccga tattttttat
     7381 gatttctatt ataaatattt tcttgaaatg aatgttgagc cgaacacggc acatattgtc
     7441 cttgccgaaa tggagaaaaa aggtaagctc tcctccgtca taacgcagaa cattgacggg
     7501 cttcatcaaa aggcaggcag taaaaacgta attgagctgc acggaacgac ggctgttcac
     7561 tattgcatgg agtgcggcaa accttatccg ctcgaaaaac tgaaatctct caagggtaag
     7621 gtccctcact gcgaaaaatg cggcggactt gttcgacccg aggttgttct ttatgaagag
     7681 cctcttaatg aaaaaacagt tgaagacgca atcagcgaaa tctcaacggc agatttgctc
     7741 attatcggcg gcacatctct cgccgtttat cctgcggcta tgtatatccg ttatttccgg
     7801 ggttcaaaca ttgtgcttat caacaaagga aaaaccgacc ttgactcaaa cgccgatctt
     7861 gttttcagag acagcatcgg tcaggttttg ggaagcatta aagaaaaaat ttaaggcggt
     7921 gatttatttg tttagagaaa tgagaagaat taagcaacag ctctcaaaag aggattgtga
     7981 agacattctt cttcgaggca aaacgggagt gcttgccgtt ttaggtgacg gcggctatcc
     8041 ctataccgtt ccgataaatt attactacac ggacggtaaa atttatctcc actgtgcaaa
     8101 aacagggcat aagctcgacg caatcaaaaa agagcaaaag gtttcattct gcgtcgttga
     8161 ccgtgacgaa attctccaag agaaatacac aacgcttttt aagagcgtcg tagctttcgg
     8221 caaggcggaa attctaacag acgaaaatga gatgcgttca tccgtaacgt cactcgccga
     8281 aaaatactgc acggactgtc tcgacggaat ttcggcagag gtcgaacgcg gattcggcat
     8341 actttgcatg attaaaataa acattgaaca catgaccggc aagcaaggaa aagaactttt
     8401 gaaggaggaa atccgatgaa aaataaagtt atcaggttaa cttcatttat tctgacgatc
     8461 attatcgcaa tgagtacaat tttctacacc tcggcattcg atgaaacggg acagataaaa
     8521 ataacagagc ccactcccct gctcaaagag gacggcagtc tcactcagcc cggatattgc
     8581 ttcacggagc tttatcaata cgaccgcagt gcgattaaag cgaacaagac ccgcatcaag
     8641 gaatgggatt tctatcaaat ttcaaacagc cgctatgtta tgcaggtcac aatggctgat
     8701 atctcgctcg gcggagcggc aaacgttcct ctctttcata tgcagaccgg caaaagctac
     8761 agcgcggtct cgctcagact tctgagcttc ggaagattcg ggctttccga aacggctatg
     8821 tcaccgcaga ccttttcacg tcacaagctc ggctttaacc ttgacgtaaa cgtgaccgaa
     8881 acccggcgca ctataaagtt caacggacgc acgggcggca agcctttcac cgtcgatatt
     8941 tcaatggata tgttcccgaa ccatgaatat cttgcaatgg ctctcccgtt cggcgacaga
     9001 gacagcaaaa agttctatta caatcagaaa ataaactgta tgcccgtaac gggaacggtt
     9061 aaaatcggcg atatgacagt tgaatttgac ccgtcggatt cgttttgcgt tctcgattgg
     9121 ggccgaggag tttggcccta ccacacaaac tggtactggg gcaacggcag cacgaagctt
     9181 gaaaacggtg acatcttcgg ctttgaaatc ggctggggct tcggcgatat gagtgccgcc
     9241 acggaaaata cacttttcct caacggcaag gctcacaaaa tcggtgaagt ctatcttgaa
     9301 aaagacgaaa acgactggat gaaaccgtgg aaattcacct cgtccgacgg ccgttttgaa
     9361 atgacgatga ccccgtttta tgacaactac actaagtcaa gagttctctt tatcggcaac
     9421 atctgccacc aagttttcgg caaatggaac ggcaccgtca ctctcgatga cggcacggtt
     9481 cttgaaatca aggacatggt cgccttctgc gagaactctg ataatatgtg gtaaggagaa
     9541 agcacataat attaatgttg aaaagataaa agagcttcat tccacacata aggctgagtt
     9601 caagcagttt atcagatacc aaaatttttt caacaattcg ccggctcatg attggcacga
     9661 aagctatgcc attatcttta tcgccttcag cattgcggca ctgataatca ttgcaatcat
     9721 aaaaagcttc ttaacgaaaa gattaacgca caagaaaaaa cgataaacaa aagaaacaac
     9781 tttcccacaa tttttttgca ggaaagttgt tttttatttg aaaataattt ttattttatg
     9841 ctcaccgggt gcggttgaaa tcctgaaaaa tccttttcgt tttattgcaa tttcttcgcc
     9901 gtcaagctcg gcatatttcg gcacggacgg aacaaaaatt tccgtctttg cttttgtttc
     9961 ctcaagctga acaaagctga gatgaaactc cttattctca cggtcgtagc cgtagctttc
    10021 aatctcgccc gtgacagcca cgggatgcgg acgggtgaga acctttttgt aaacctccgt
    10081 atccgtaaag ccgccgaagt agcaccaata ggtattgctc catttatatg aatcgaaaag
    10141 gtcgagcaaa aattcaatat gcgggaacca ttcgtcaccc tctgtgaagc cgccccattc
    10201 accgactata acggggactc cgagccgctg ctgagagcgt ctgtgctcgg caaaaatcga
    10261 accgacacgg tcattgcttg cgtacttata cgacggagtg tccaccataa aatcgtaagc
    10321 atgcggagaa aaaataacgt tgttatccgt cctgcccgaa agtatcgggc gtgggcatga
    10381 ataaggaatt ccgagattgg aatagtagct gttttcaaga aaaacaaatt tttcgttgcc
    10441 gcattttctc gtcgccctcg ccattttatt tatgaacgga aaatactttt ttgtgtcaaa
    10501 ggttttgata atatcatcgc cggcacttgt gatttttctc aggtgtttgc ctgtgtaaag
    10561 gtcaagcact cttgccggat cgtcgctctt tgtcgcaagc tcgataagct ttttctttga
    10621 tatcgtttta tccaaaacag ttgttgaaac aagtcgcccg attatttcac gaaagacctt
    10681 gcctccgctc ttgcccggaa acggctcgtt gaaaaaatca aatccgatta ccgccggatg
    10741 atcgccgaac ttttcaataa catgcgtcca catttcctca aaccaaacct gtacgccctt
    10801 accgttaact ttggaattat tccaaaaaga atcaaatgcg ctgtgaactg ccttgcccca
    10861 gaaatagccc tcagcccaga cgattttcgt cggctgatat ttatattttc ccgtcagcgt
    10921 agcccatttc ggcgcaccgt caccgcagag cagctccgta cccgaaaaaa gatcctggtg
    10981 catatcaaga aaaacataaa ctccggattc tttacattca tcaagaatac cgccgataaa
    11041 atcaagatac tcttcgttgt attcacccgg ctcgggctcg attgcgtccc acgttattcc
    11101 gaggcgaaca agatccattc cgtgagcctt catgtcggcg gcaagtccct ttgtccattg
    11161 ctcaccgtag atgcgtcttt gaccctcgat atatctgccc ttatcgcaca cattgagtcc
    11221 ggtcaaaatt ctttcacgtc cgtgagaatc aactattcgt gtgcctttta ccgtaagttt
    11281 atccattttt gcctcctttg tttctgcgct gttcctttgc ttttgcagca tactccctgc
    11341 gcttcgcctt aagttcctcc tcctcggcaa tgcggcgctg aatttcctct tcattgcgtt
    11401 tcttggattc ctcataacga gcttttatat cctcataatg aagataatta tctctttgct
    11461 tcaagagctt ttcagcttca atatacggct ttgccgctct ggtgtaggtt gaattttcat
    11521 ctgtggcaac cttgattttt ttgcggattt cgtctcggct ttttctgaga gacttgattt
    11581 tttcgttcgc ttttttctcg gctgctttat ccttattttt tgccgccgtt ctcagctcgt
    11641 taaccgtctc tttgatctga gcatcaagct cgtcttcctt ttcaaagagc tgctcaaaaa
    11701 ctctgacgtc gaatttttcg attccgtcgg tatatttctt tgtcaaaacc tttttggaat
    11761 ccctgatcat gcggactctg tttatcgcat tttgccgctg ttccttttca ttctcgtttg
    11821 atttcggcat cgccttcgcc gccttcatcg acggaacctc aagatcgtaa aggccttcaa
    11881 gccctgcgtc gtaaattttc tttgcctctt cgagctgagc cttgatcgct tccgtttcaa
    11941 acttgtttat ctctttaata acatactgtg caatttcgat tttttcgttt tcttcttttt
    12001 gatttttata gctttgcttt gctgccttca cggctgattt atccttggct ttcttcgcct
    12061 ttttgatttc atccttcgtc ggcttcacga tatttttatc gtgataaatc tttgcttcct
    12121 cgataatgtc aatcgcctca acaagggcgg catcggaaag aactccgttg ccgtaatcct
    12181 caaacaatgc tctgattttg agtaccgtta ccattgcctt ttgcttaatc tcggtcagat
    12241 catagaagaa atacggtata acattcagcg ttgcgcccac agttgcggca ataattaaaa
    12301 cgctgcaaat atttttgaaa taccacgggt cggaaagaac ctcataaaca ttgcttccgt
    12361 caaagccgag gctttttgca acctcctcat taagacccga acggtcataa agctccggaa
    12421 gcgcggcatt tgtaataaga gtaactatgc ttccgatagc accgacggca acaaacattc
    12481 cgtcaattcg ctctcccgtt atatattgct gatagtctct gatatctccg ttaacgccgg
    12541 gtgttaagag tgttacggcg tatgctgcca tataattaaa gaagaagcag aacataagcg
    12601 gccaaataat ccgggaagga tcaccgagtc tgattatcgg cagcatcagt ataataaaaa
    12661 ttacatttac aacattgctg acaacaagaa tttttctctt gccgagcaca cggataagga
    12721 acggaataaa aagcatcggc caaagtgacg cattgcctct tatcgtgaca atgagtgaat
    12781 attccgcagg actgcatgca tcctgatagc tgtagagcca gtccatgata ttctgaacgg
    12841 cattttcaag aaaacctatc cagcctgcga gcgaaatgat ccaaaaatat ttattgcgaa
    12901 ctacggctcg aaatgcgtcg gtgaatttaa tttttacagt gggagttttt gcctgaacga
    12961 ttttttcttc ggtgttgaaa taaacaagca gtccgaggaa gaaaccgaaa attatcatcg
    13021 gcggataaaa tgctctgtaa attctgatgt caacaagcgt gttttcgccc gttattgccc
    13081 ttgcaacaag cggaggtata atgttgccca aggttggcgc aagcgaattg ataaccgatt
    13141 taatcgacgt tacgtcactg cgctcgaacg tgttcggcga aagaacattg atgatgcttt
    13201 gatcaacatc ataatagaac atatagaaaa actgcaagcc gatgttataa agcaaaacag
    13261 tgatacattt tgcaagcatc gacatacgtt catacggcat aaagacaaag ccgattgcaa
    13321 gaagcgccgt cggcagaccc attgaaagaa taaacggccg atagcgtcct tttttgctcc
    13381 tcgacgagtc aatgattttt cccctaagtg ccgtcagcgg aaagcttagg ataacactga
    13441 gaatatatat tatgtacaaa ggcttcggcg caataccgat ggtgttgctt atcagcgtgt
    13501 tgccgatgct gattatcata ttctgcgcac agaaaaccac aaattgaatt ccgagtccgc
    13561 ccacggcaag cgacacaatc tctttaaaag acatataccg acccatcggc ggctttttcc
    13621 agtataggcg caaatcttca cgcagcgttg tcgctttttc ctttatattt attcccatga
    13681 gcatcactcc gtccgaaatt ataatttgat tatatcatag cagaaaataa aagtaaatcg
    13741 gaacattata taaaaaaaca ctccgaaaat tttacatcga agtgtttata ttatcattca
    13801 aaaatatcaa gttttaattc atacagcaac tcatttagtt ctatgatggt cagcctattc
    13861 tcaagagcaa ataaaatcac gttgtctctt ttgttctttg cataaagaag cggagcagag
    13921 cattcaagca gaaaatgctg agcttcctcg gctgtcatct ccagtccgaa gcagaacata
    13981 agcaatttat cccgtgaagg agttttttta cccgaaagaa tttgataagc gtaatgtctt
    14041 tcaatgccgg aacttcttat aacctcagct tttgtaagtt tccgcttttc catttcttca
    14101 tttaacaatt cattcggttt tccggttaaa aattccgtct cattcgcttt tatatattcg
    14161 ttaaggctgt tgcatttttt tagcatttct tgcagttgcg ccgtggattt tttcatgtta
    14221 taatactcct atctgaaaaa gtatggcggt gattttttga gtgagcttgc gcaaaagctt
    14281 ttcgtgtgga acacggagga attaaaggat caatacaaag ctatattgac gaagggaaaa
    14341 tattcagcga agaagaagcc ttgaaaatcg ctcttcaaat ttgtgacggg cttgaaaagc
    14401 ttcactcgaa cggaataatt caccgcgata taactgccgc taatattatc ctctcttatg
    14461 acggtaatgt caagataatt gactacggta ttgcaagagc cgcaaaagag aacgcttcaa
    14521 gggatacata tatcctcggt acggcaggat atgcagcgcc cgagcaattc ggtttttcac
    14581 aaaccgatga aagaaccgac atatatgcag tcggcgtact ggtcaatatg cttttgacag
    14641 gcaagttccc taatgaagaa atgtgctccg gaagatttaa aagaattgtt aagcgatgca
    14701 cttctatgga ttcttcagac agattttcaa gcgtcaatga attaaaaagc gagctgtcgg
    14761 gcccaaaagt tttcttaaaa aatgacgacg gctttcccgg ctttagaagt caaaatttga
    14821 ttataaaaat tctggcatcg ttgatctatt tcataattat ttttatagac tgcgccttgg
    14881 tttcgtccac ttataccggc aaaggtttgt tccgtgcagt tttgcaatgt gttaatctta
    14941 ctgtagcttt tgccgttccg ttttgcctga tttgtaatcc gtatgatatt caaggaaaaa
    15001 tcaaacactt ttccgcaatg aaagattcca caaaa
//
LOCUS       DAWBSW010000028        14640 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_892_length_14640_cov_2.737333, whole genome shotgun sequence.
ACCESSION   DAWBSW010000028 DAWBSW010000000
VERSION     DAWBSW010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_14640_cov_2.737333"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6594..6603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11449..11458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14278..14287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4044 a         3160 c         3499 g         3907 t
ORIGIN      
        1 atccatgttt tcagagaggg tcaaatagtt aaaaccttcc cgcacagaga agcaacagaa
       61 gaaaatatca tcaaagcgtc tacggcggat atcgcctgaa ttaggaaagg aacgtgaata
      121 ccatgtctga aaaaataaag caggttaaac ctaaggaaaa actcactttt aatatgatct
      181 ggcagaagta cggaacattc gggattcttg taatcttgct tgtaattctt gcaattatga
      241 gacccggaac cttcttctcc acagcaacga taacacagat tctttcacag agttcggtta
      301 acattcttat tgcgatgggc gagttcttcg caatccttat cgcaggtatc gacctttcgg
      361 tcggctcggt tgttgctctg accggtatgg tctcggctca gatgatggtc gcaggcatca
      421 atccgctgat tgcagttttg ctcggcataa tcttcggcgg cttactcggc ttcatcaacg
      481 gacttcttat caacgtaacc ggacttcatc cgttcatcat cacgctcggt acgcaatcga
      541 ttttcagagg cgtaacactt atcatctcaa actcaagcgc agttttcggt ttcccgaaat
      601 catttacaaa cattatttct tacaaagtat ttgatcttct cccaatcgtt gcgataatcg
      661 caatcgttgt agcgcttctc ctctcgttcc taacaaggaa aacaaaggtc ggcagaaaca
      721 tttacgcact cggcggcaac aaagaatcgg cttggtactc gggtatcaac gttaacatgc
      781 acacgcttat cgtattcatt atctcgggta tttgcgcagg tatcgcaggt atcgtccttc
      841 tcggacgtgt cggttctgct gagccggcag ccgcaacagg atttgaaact tacgcaatcg
      901 cagcttcaat catcggcgga acaagcttct tcggcggtaa aggtaagatc ttcggcgttg
      961 taatgggcgg tctcatcatc ggcgttatca actacggtct taacattctc gcagttccga
     1021 gcgtttggca gcaggttgtt atgggcttgc tcatcatcgg ttccgttgca ctcgacagat
     1081 tcgtagctcg caagaaataa tctaaccgca aagaggtgct ttttatgaaa ccaatgttta
     1141 atccgtcgct tatgtgcatg gatctccttg atataaaaaa tcagacggag atcctcaacg
     1201 aaagatgcga cctctatcat gtggatataa tggacggtca cttcgtcaag aacattactc
     1261 tttcacctga ttttgtaaaa gctttctcaa ctatagcaaa gaaacctatt gattgtcact
     1321 tgatggttac aacacccgat gattacatcg aaaatctcgc taaggcaggg gcaggctata
     1381 tctgtcccca tgccgaaacg atagtcaacg acgctttcag aatcatgaac aagattgagt
     1441 cgctcggctg caaaaaggga gttgtgctca gtccgtcaac tccgctctcg gcaatcaagc
     1501 attatatcaa caaaattgat aagcttacaa taatgtctgt tgaccccgga tttgcaggac
     1561 agccttttat cgaggaaatg cttgataaaa tcagcgaggc gagagagctt aaagaaaagt
     1621 acggctataa ctatcttatt gaggttgacg gctcatgcaa tcgaaaaacc ttcaagcgtc
     1681 tttacgacgc aggaacggaa gtgttcattg tcggttcctc cggactgttc tcaaaggatc
     1741 cggatctttg caaggcatgg gatatcatga cagatgaatt caaaaccctg acaggcgcag
     1801 aaatataatt gattacctgc caaaaggagg tggacaggat gggaaattta actttgggaa
     1861 ttgacatcgg cggaacgaat ttcaggctcg gtgcagtcga cgaagaaggc aacatcagtc
     1921 actttgaaaa aaattcaagc agagtttttg atgcgggcga tgttgtggag ataattcaca
     1981 aggaaattga ggattatatg gacagatacg gaatccgtga ttctgtcaga gccgcggcgg
     2041 tcggaatacc gtcaatagtc agcaaggata aaaagacaat tatttcaacc ccgaacctta
     2101 aagggttcaa caatatccga ttcagcgaca agcttcagga aaagctcggt attccggttt
     2161 ttcttgacag agatgtaaat tttctcctta tgaatgacat gaataagctg aaaatcgaca
     2221 atactaaaac cgtgctcggt ttttatgtcg gaaccggttt cggaaatgcc atctgcatca
     2281 acggcaagct ctatgccggc aagaacggtg cggcaggcga gcttggacac attcctcttt
     2341 atgatgttga tgaggaatgt acctgcggca atatcggctg ctcggaaaca agatgctcgg
     2401 gcaagagact tgaatacctt gcggaaaggt atttccccga tgtttcgata agagaggttt
     2461 ttaagtatca cggaaattca ccgattattt taaaatttgt caaggatctt gcaattccta
     2521 ttgcaacaga gataaatatt cttgatccag attattcaat aattgcaggc ggagttacct
     2581 ttatggaggg ctttccgaag gacgttcttc tcaaagctgt ccatgaaaag gtgcgcaagc
     2641 cttacccgga ggaaaacctt gaaatcatgt ttaccgagca tgaccagcag agcggaattt
     2701 acggcagcgg aatttacgct caccaaatgt taaagaatat tatctgacgg tagattgatc
     2761 cgttggtagt attaaaaaaa cggaagctgt taaaacagtt accgaaaaaa ttattcaaaa
     2821 cagaaaggat aatcactatg aaaaagacac gaattctcag cattatttta gcggcagtta
     2881 tggtatttgc acttgtactc acaggttgca gcacaggcgg caatacagac aagaacacag
     2941 gcaacgcaga ctatgcagtt attctcaaac cgctttcaaa tgacttctgg gcaaagatga
     3001 aggccggtat tgaggaagaa gcaaagaagc agggtgttac tgttgatatc tttgcagcaa
     3061 caacagagga agacacagaa ggccagctca agatccttga aaactgcatc aacaagggct
     3121 acaaggcaat cggtgttgct ccgctttcac cgacaaacct tattaacggt attgttgagg
     3181 ctaacaagaa gggcatctat gtaatgaaca tcgacgagaa gatcgacatg gatactctta
     3241 aggcagcagg cggcagcgtt atcgctttcg caacaacgga taacgaaaag gtcggcgaaa
     3301 agggcgctca gtatatcatt gaaaagcttg caaacggcgg cgaagttgct atcatcgagg
     3361 gtaaagcagg taacgcttcc ggtgaatcaa gaaagacagg cgcaacaaac gcattcaatg
     3421 ctaactcaaa ggttaagctt gtagcttctc agccggcaga ctgggatcgt cagaaggctc
     3481 ttgacacggc agcaagtatg cttcagtcaa atccgaacct caaggctatc tactgctgca
     3541 acgacacaat ggcactcggc gcacttcagg ctgttaagaa tgcaggcaag gacgttatcg
     3601 tagtaggtac agacggtgca gaagaggctc tccagagcgt tcaggcaggc gagctttcag
     3661 caacggttgc tcaggactcc gcaggaatcg gtgcaacatc acttaagcgt atgatcgaag
     3721 ccgttaagaa taaggagact atcgacgcta acgcaactcc ggaaacaatt ccggttgact
     3781 ccaaccttgt tacaaaataa ttaaataagg ttctttcctc cgaactttat tatatgtaat
     3841 ctgtagatta caaaagctcc gggacaccta cggcatactg cgtcccggag cacgagcaaa
     3901 aaaatgacgg ctcgatcggc tcacttcttg atatattttc cccttctatt ttccaaaaac
     3961 tgctttacga gtttagatcg accgacattt tgttgccgcc gcattgttaa taaacggtgt
     4021 ggcggcaaca ataaagcata ataaccgctg attaaatttc agcacggtac agcgaatgcg
     4081 tgccgcaatt taatcaacga ttatttgaaa ggtggttttt ttcatgaaaa ttgcaatagg
     4141 ctgtgaccat gtcggatttg agttaaaggg caaggtcatt gagcatctta aagaaaaagg
     4201 aatcgaagtt gttgacttcg gcacaaacag caccgagcgc acggattatc cgatttacgg
     4261 caaggcggta ggtgaggcgg ttgcctccgg tgagtgcgat aagggcatat tgatttgcgg
     4321 cacaggcgta ggtatttctc tggcggcaaa caaggtaaag ggtatccgcg ccgttgtttg
     4381 cagcgagccc tattcagcat tgctttcaag acagcacaat gatacaaatg tgcttgcctt
     4441 cggcgcaaga gttgtcggac ttgacctcgc tttgatgatt gttgacgcat ggctctccgg
     4501 cgtttatgaa ggcggacgtc atgcaaagag agtacagatg cttgcagata ttgaaaatga
     4561 aagataatcg gattgcaccg aaaacgaccg ttgcagattt tttgcggcgg tcgtttttcg
     4621 gtgccggaac cgtgtatata cagatctgag atacctattt aaaagcagca ataagacata
     4681 aatctccgta aagtgcatat ttcacaaaaa aaaccaacta atattgtcta ttaacaccaa
     4741 gtcaagcacc ttgcactttg acggaaaata atttataata ttatttgtga tggtatagtc
     4801 aattgttaga ttttgtataa taggagtgtt gcttatgaaa cgtaggatct tatccgtcgc
     4861 gatagcgatt cttattttga ccgtctcggt gattccggga ctttccgcgc tggcggcagg
     4921 tacggtgccg accttatcgg gcggagttta tcaaatcgga accgcttctg agctcatttg
     4981 gtttgccgat gccgtaaaca gcggctcaca gtcaataaag ggtaagctga cggcagatat
     5041 ccagctcaat gctcccggct ctgttgctaa caagtggacg cctatcggta cgcaggcaag
     5101 tccgttccgc ggaacctttg acggtgacgg acacacggtc agcggagttt atgttgattc
     5161 gggtattgac tgtgcgggat tcttcggcag cgttgtaatt ccgagcaata cttccggtga
     5221 tgcggacgct cccgataaaa tgacttcgga atatgttctt cagcacactc agacaagcat
     5281 taagaatata aagataacaa atgcggaaat tcacggcggc tacagcgtcg gcggtattgt
     5341 cggctatgcc gagaatctcg gactttccga ttgctccttc gagggagatg tttacggctc
     5401 gggtaacagc atcggcggca tcgtcggttg ggcatactat tatacggtta tcaatcagtg
     5461 ctattcttcg ggaacagtca gcggtaatca gcgtgtcggc ggcatcgcag gctatgcaaa
     5521 cggcaactcg gttatagtaa agagctacag cgatatggct gtttccggca aattgaatgt
     5581 cggcggtgtc ggcggaactc tctcgggcgc attccttgag ggctgcttct tcctcggctc
     5641 tgtttcggca gatgatacta tcggcggact tgtgggctac gcccttttct caacagtttg
     5701 cgacgcctat gtaatagctc ccgttacaag cacgggaaaa gaggttggcg gtacgatagg
     5761 ttccgtttac ggcagcgaat gtgacagcaa cttctacagc tatgagactt cgggcgttga
     5821 cggcacggca ggcgtcggac gtacattggc agacatgaag ctttccgcct ttgtaaaaga
     5881 actcaacggt caaaaagcat acttctgctt tgactatacg gagatcaata acggttatcc
     5941 cgttcttgca tggatgcttt cgcttgatgt ttgggcaggc gaccgctctg ttccgcagaa
     6001 gaattcaagc ggtacatatc ttatttcaaa accgtcggag cttgcatggt ttgcggcttt
     6061 ggtaaacggt cagctcaacg gcatggatgc aaatcccaat gccaacgcaa ccgttacgga
     6121 caaccttctt atgaacatca atgtcaatga tgacagcttt ggtgttattg aatggacgcc
     6181 gataggcatt gacgaggcac acggatataa cggcattttc aacggcggcg gctacaatat
     6241 cgcgggtctt tatacgtcat caagctcggg cgacagcgga agaaatatcg gtctctttgg
     6301 atatatcaat accggtacgg tcacgaatac ggtcgtgctt gaaggcagaa tctcatgcat
     6361 tgaaaatgtc ggaggtattg ccggctattt aaaaagcggt tcgattctta actgtgcggt
     6421 tgacagtgaa gtaagcggtg ataaggcagt cggcggaatt gtcggcaatc tcggcggctc
     6481 aacggcaagc gtatcaacga gcagtatgct cggcagcatc gtgggtacaa attattccaa
     6541 cgataaagcg ttcctgacaa atgtcggagg agttgtaggc atcagcaata aggnnnnnnn
     6601 nnngcaataa ggcaacggta aacaagtgct ttaactatgc gacgataagc gctccgaatg
     6661 caagatttgt cggcggtatt ctcggctaca actcagccgg aacacttacc aacagctaca
     6721 gcacagcagc tcagacaagc ggtaacgtca tggtcggcgg acttgtcggc tacaacaata
     6781 acggcacggt caaaacctgc tatgtttcaa gcaaagtaac gggttcaagc caggtcggcg
     6841 tcgctttcgg caatactgtg ggtgcaaatg tttcgagcat tatatttgac ggaacatata
     6901 aaacaaacct gttgacaacg ggtgcaacgg aaaaaactcc cgcccagatg acagggtctt
     6961 cctctgtttc aaacatcgga ttttcaagct cagactggag agcaaccgcc gatgatacgt
     7021 atttttatta ttatcctcag cttacatcaa tgtacaacat gtcctccgtc aaggtgataa
     7081 aagacggttc cgtgaggagc gtaaagagag ttcaggacaa gtatgttgca aaggttcaga
     7141 tagacggacg cacggataca tattatgaaa ccgttgcggc agctattgac tatgcttcaa
     7201 acacaagctc aaccgttctt ccgacggttt atgttatccg tgataccgag cttgcctcaa
     7261 ccgtaaacat cacctcggaa atcggaattc tctccgagga cggcgcaacg ctcacaaggg
     7321 cggcttcgct taccgctccg atgttcaatg taaccggcaa gctgaccttg ggctcaaagg
     7381 tctatggaga cgaaagctcg gctaatttct atatcgacgg caacaatgtt gcggcaacgg
     7441 cttctgcaat cagggttgcc gagggagcaa cgcttacggt tcagccgggt gttgaattat
     7501 atgacttcaa aaccgcagag cagtcagcgg caaccgtaat gggtgcagtt gtttataacc
     7561 aaggtaaaac ggagatcgtc ggcggtattt tcagcggaaa tatcggcaag tctgtcggcg
     7621 gcgtaatata taaccttgag ggcaccgtaa aaatcagcgg cggcatattc agatcaaatg
     7681 aggcgacaca gggcgcagtt ctttacaata acggcggaca ggctgtcatc acaggaggct
     7741 catttaccgc taatattgca aagctgaagg gcggtgttgt ggcatcgtac agtgtttatg
     7801 caacaacggc tgtcggcgga aaggcttcaa tgaccactaa tcaggcaacc tatggcggcg
     7861 cgatttgcgc aatgaattat tcaacggttg aaatcagcgg cggtacgctt tcctctaacc
     7921 gtgcatattc ttcgggcggt gcaattcaga tagaaacagg agcagaggtc agaatctcag
     7981 gcggcgatgt ttcaagcaac acctcggata aggcactcgg aaatgcgatc tataacggag
     8041 caacgctctc tctcgacgct gcggctcaga tcgactcttc aaatgatatt tatcttgtca
     8101 gcggcaagac cgttgatata accggcaagc tcacatgctc cggatttgcg gcaactgtaa
     8161 cgccgcaaac ctacgctgaa aagacaaaga ttctcggcgg aagtgcaatg agcaccaact
     8221 atatgaaatt cggactttca aattccaact ggtacacgct cgctaacggt acaataacca
     8281 gcatggagac cacaacggtc gcaagggtaa gtaaggcagg agccttttcg gttgaataca
     8341 ccgatttgtc cgatgctttt gcggcagtct cggcagatga gacggcgata attacgctcg
     8401 ttgccaatgc ttcggtgacc tcaacaatta ccgtcaacgg cgacgtaact cttctctgcg
     8461 acgatgcggg ctataaggcg atcagaacag gctcattcta cggaataatg tttgatatca
     8521 agaaaggcgc aacgctgaga ttgggcaatt ccgttgccga taacgttcag cagtcccaga
     8581 gcgactatga atcgggcacg gattccgacg gacttatgat aatcgacgga ggcaaggagc
     8641 tgaccggagt tgtcggtgca gcggctgtaa atgtccagac gggcggaagc ctttatatgc
     8701 acgagaaatc cgcggttcag aattgtaaga acaccacgac cggtgcaatt acagtttccg
     8761 gcacaatgta tatctacggc ggaacggtaa gaaataatga gtgtaattac ggcggcgttt
     8821 atgtcaaggc agggggcaag ctctttacct acggcggcgt tatagccgat aataaatccg
     8881 caacgagcgg agacgcagtc tattccctcg gcaaagttac aagggttgtt caaacataca
     8941 gttacctcta tgttgagaag gaatatgacg aagacggcaa cgttatcggc tcaaaagatc
     9001 ccgttgcggc aggctcttca aagacagata tattgattaa gaacggcgac gcggtctaca
     9061 attcaacgaa cattatctat attgacacgg cgacgacgga tatttatatc acaagtatgt
     9121 cgcaggttcc cgaacagacg gagttcaaac gctatgtcat gactcttgac tgtgcaaagt
     9181 acactgtcgg caatgtgatt gtttcgggcg agaatgtgat agacagctac agttatttcg
     9241 acacctacgt tgacggctat tgcatacttt atacgggtaa gcttggcata aacaagcttg
     9301 ttgcgaaaag cacttcggga ttgattgttg acagaaagaa caatctggtt tctggcttca
     9361 atttcaataa tctgaccgta agcaaggtag tttcactctt tgaaaatgag gtagtttacc
     9421 tcagagttta tgatgcaaag aacaccatgc tgaaaaacac gaataaggtg acaacaggct
     9481 gcaccgtaag gctgattgac gcttcgggca atgtgattga ttcactcact gttgttattc
     9541 gcggcgatgt caacggtgac ggctacatcg atggcagaga ttctgttatt gtcagagcaa
     9601 ttgcaggagg aatgttgagc ggcgataatg tgactgcggc tcagacggcg gcaggcgatg
     9661 tcaactttga cggaacggta acttccgttg atgcagaaca tatcgacctc gcaggcatcg
     9721 gactgcaaac aatcgctcaa aaagcttgaa cggttaaaac gggaaattga tttttcatgc
     9781 aacaaaacag tgatttaaga catccgaaag ggtgtctttt ttcatatctt cacttcaaaa
     9841 gtctgtgaga aagagagtga ttcaaataca agatgtaact tgtaaaaatg caggataaca
     9901 ttttattgca aaaatgacgg ttgaaagctc cgttaaatga gtctatcgag tcttttataa
     9961 gtgtaaacac tttgatttac tctgaaacac cgacaggctt cggaaaaact ctttcggtaa
    10021 ccttgaaccg atataaagag aaaaccgtcg ccccagattc tgaacagatt acaataatcg
    10081 gaagtgatga tttgaaagga tttgaaagta tagctgtcgc tcagatagaa ttaaacagat
    10141 taagaaaacc caaaacaaga aaaaagcctt atttatcaac gttttcattg ataaataaga
    10201 cttttaataa gtggtcggag tggcgggatt tgaacccacg gcctcttggt cccgaaccaa
    10261 gcacgctacc atctgcgcta caccccgaca acgcttgaat attatatact ataatcatca
    10321 taaaatcaag tgtttttcta aaaaattttg atttttttta gaaaaacaga tgaattgaga
    10381 taaaggaaaa ataaatgcgt tagtgatagg acatctattg cggtaattgc ttacttcctg
    10441 tactgttatt ttgcagagct tttttgccga aacgtcgcag gtgcgtcact tgactgagaa
    10501 tccattgact ttttcgagct gtgggcgtag aattacggca aggtgaaagt gcgggaacgc
    10561 tgaggtgaac aggcgccgtc cgtcggtgtg tttccggctg tcaactttaa cttgttgaaa
    10621 ggattgatct tatgatcgaa aaaaatatgc ttggaaaatt catcagaata aagagagaga
    10681 atgccggact ttctcaaaag cagtttgccg aacagctctt tgtgaccgag tcggctgttt
    10741 caaaatggga gaggggagtt aattatcccg acatcacaat gatctccgac atttgccgtg
    10801 tgctgaatat aagcgaaaag gagcttatca cggcttcgga tgacacagca tatcggcaga
    10861 tgaaaaagca gtcggcaaaa tatgagagaa ttcaaaagct tacgttttgg atcccgacct
    10921 cggcatatat ttttagtttt attatttgcc ttatttgtgc cttatgcggc gtcatgccgt
    10981 tttcgaccct gttcattgtt acggcgagtc ttttttgtgc atttcttttc ttcccgtcgg
    11041 ttacgaattt tttcagtaat caaaagatgc ttgcctatct tgcttcatcg gcgataggtg
    11101 ttatattgtt gcttgttgta tgcggaatcg caacaaagag cattacatgg gtcccgacag
    11161 cggtcatggg gacattgctt ggatattctg ccgtatttct gccgattgtt ttaaaaaaat
    11221 attcaaaagc aggatttgtt tcaacgcata aaacatttat aagcattatc gccgatacgg
    11281 tgctgacaat cattctcgtt gcaacggttc aaattcttac accgataaat ttacgcagtg
    11341 cggaactgac ggtgctttat tgtttctgtc cgttgctgat aacaggtctt gttgtcagtc
    11401 tcagacttaa cgggctgata aaaggcggaa tttgtacggt tatattcgnn nnnnnnnngt
    11461 atttatgctt tctttgcgaa ctattttgtc ggcttggttt atgatgttcc gacagacatg
    11521 gcaatcaatc tgtcggattg ggcaacatat accaacggaa atgtgctgct gactgtgctg
    11581 gtcacatgcg ctgcggttgg aataggtctc atcggctgtg gaataaagaa aagcagaaag
    11641 taaatagaaa agggctgtga cattttttgc cacagcctga ttttatttca atatattttt
    11701 ttcaacatac tttgatgtca ttgcgttgag caggcagttg taggtcattt taaaatctac
    11761 gaccgagccg actttataat cgtcgccgca gtcggttacg tcaagaacaa cgtgatcact
    11821 gctgccgccg acagttttca ttcgcttgtc tgcacagata agtccgggca ggtaaatatc
    11881 ctgcttgccg atcgcaacta ttgcacgctt catcagcttg tcgcagtcct gcggaagagt
    11941 ttcgggtaca ctcggctttt ctttcagctc gatgatttcg gctttaagaa tgaaattgtc
    12001 ctctctcgct ccgtcaaagg gaacatcata aacgggaatg ttgccaagaa aaatcgactc
    12061 acccattcgc atattgttga tgactttcgg caatttgccg tcaaaaagca tatttattga
    12121 gcttgacgca ccgccggaaa ctatcggaag gttaatattg tattccttct gaacttcatt
    12181 gacggcgttc tcaaacagag ccatgttttt ttcactcggg agcacctcgt ttacacaact
    12241 gaggttcgtt cctacgccgt aaagctcaag gttcggcatt ttgaggatct cacccgcaag
    12301 agacttgagc tcgtttgtat agcagccgtc acgcaagtcg ccgcattcat acatgagaat
    12361 gaccttatgg cgcttattct gcttcttcgc ttcgtcattc agtgcacgga gcacatcaag
    12421 ctcgctgtta aggctgacgt cggtgaactt cacaacgtcg ctcacctgcg aaagcatagg
    12481 agagcgaatc agccattttt cagccttgat atcaacaaat tccatgaagt tttcaactct
    12541 ggaatcgcaa attgccttga ttttgccctc ctcgacgagg tagcgcacaa gaggctcata
    12601 gccgacaagc atcttggtaa cgaccgagag agacattccg ttttctgaac atctttgctt
    12661 cattatttta aggttatgaa gtattccgtc cggtcttatt tcgatacatg gaaacatatt
    12721 tttactccgt tttctgatct atgaaacgtc ttacggtctt gaacgaattg cgctcgattt
    12781 caaaaaagct ttcgtcaagc ggcttgcacg gttcgcagcc ctttgccttg agagcctcgt
    12841 tgattatctt ctccgtgtac cacggattct ttacgaatac cggaccgagc gcattggtga
    12901 agataacgtt tttgtatttt gcaccctcgg tttgatcctt gtcattgttg cccttgccgt
    12961 aagtgagctt tccgagtgcg attgacgggt cgttaaggaa gaaatcaaca aggtgaatct
    13021 gaaccgccat gatttcaaaa tttttgtcct cgcagagagt gaattgagga tcgtctctgt
    13081 aaatctcgtt gcgttcaaca gcagtggcgt caagaagtcc gaggccttca acaacagagc
    13141 cgtcgcatct ttttgtggac ttgatgagaa ccatacccgt tgttccgatt gcaataatcg
    13201 gttttcctct ctcggcgaag ttataaacgg catcgccgta ttttttgatg ctttttacta
    13261 ttgaagctgc ggtttttatt tcgcccgcat tcataaagat aatgtcggaa ttctcaaaat
    13321 caatctcctg agcgaaggtg tcaatacggt tgattttaac gtcgagaccc attgcctttc
    13381 cgacacgctc aaaagccatg agatttccgc ggtcgccgtg gagattaagc aggtcgggat
    13441 aaagccatgt tatatttaat gtgttcatta ttcgttacct ccgtactgct ttgtggattt
    13501 ttcaagttca tgatatttct tgagccacgt gataaggtaa acattgtcaa cgtcgaattt
    13561 atcaagctcc ttgagaattg cctcgtcgct gtcgttcgga agtattgaaa tgtcatttct
    13621 gtcgataccc gcataaatca ttctgtttgc agtgtcatat gcaacggcct cggagaagca
    13681 gatatagtgc ttgatgttgt tctttttaag cgactcaaaa tcaacgtcaa aagcgtagaa
    13741 tgtgttggta tagtacgggt cgaagtcctt gagctcctcc agacccatga gaagaagctt
    13801 cggtgaatcg tcctttgcga tatagtcaag cgcagtctgg agagtttcgg ggttttcctg
    13861 tttgattctg ctatatttta gtgtcttggt cttgtatttg agggtttcca tgcggccgct
    13921 tatgttttta aagtttgaaa agctcctttg aagctcctcg tttgtcatgc cgagctttgt
    13981 gcagacggca atgcaaagcg catagttgaa gatgtagaaa ggctccttgt tcgttacggt
    14041 gaagcgatat ccgttacagt cgaagctgca ctcggagaaa tcaacgttct gagcgtagaa
    14101 atcaaccttt ttctcggatg aaaaatcgca gcctgcgcag tgaaacttgc cgacattgtc
    14161 aacgttgtaa taatcgaaaa taatcttgtc gttgcacttg gggcagggca ttgttacgtc
    14221 gtagaacttg tccttgacga aagaatactg agttttgtca acgccgtagt agtatacnnn
    14281 nnnnnnngaa aaatcctcga aggattttga tctcggctca ccgtcattga ggaagaatgt
    14341 catgctgtcg ttaaatacct tgcggaattt ctgaacgatg taatccggct cgccgttacg
    14401 ctgaacctga tccttctgaa ggtttgttat gcatatcttc ttagccggga tatatttata
    14461 aataccggca agcgaacgct cgtcgatctc aaaagagaaa aactccgttg taaccttgcc
    14521 tgtaagcgtt gaattgcgga tgagagctgt tgcaattccg cctatcatgt tggcaccctc
    14581 aagatttgag caaactgttc tgccggaatc tctgagagcg tgaactatca tattgttggc
//
LOCUS       DAWBSW010000029        14609 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_896_length_14609_cov_2.351381, whole genome shotgun sequence.
ACCESSION   DAWBSW010000029 DAWBSW010000000
VERSION     DAWBSW010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 14609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14609
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_14609_cov_2.351381"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2623..2632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3435..3444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3873..3882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5861..5870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6536..6607
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8370..8379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9722..9731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10181..10190
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10736..10745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3964 a         2993 c         3232 g         4268 t
ORIGIN      
        1 gtgcgcgccc tgaccgaaaa atatatttga aacgagcatc gaaagcgagc cgcacaagaa
       61 accgacatag ggaccgaacg atatcgccgc aaggattacg attgcacagg ttagctttac
      121 ctgaggaagc ataaaaagcg ccgcgcgtcc gacaaccgcc agagcaacaa cacaggcaat
      181 tgcgacaagc tccctcgtct gcatcttttt cttttcaagg ctcaaaaaaa gagcggaaag
      241 cgcaagtgcg attatcaacg cgctcgcaac gtaatattgt tttgagatta tcagcttgga
      301 acatccgaaa attatagcgg ggattccgag gatgaagaca aaaagagtga ttattttttt
      361 atacattcaa tcacgtcctt ttctgttatt gcgccgtcga taaatccttt tgaaattttg
      421 ttagccgcag ttgtgtaaaa acggttgccg tcaaaaaatt tatctgttgt gttgactgcg
      481 gaaatattcc tgtcaaaaag cattgcacaa agctctgcat tttcggcgca aaaatctatg
      541 tcatgcgaaa taataacaac agttttattt tttgctttca gcttggataa tatatttgaa
      601 aaaatctttt taaattctgc gtccataccc ttggtaggct cgtcaaagaa ataaatgtca
      661 cgatcacaaa gtaaaagctt tgcaacggca acacgctgct gctcgccgcc gctgaggtca
      721 aacgggtgat ttttaagaag attttcaatt tgcgtaagct cggcagcttt ttttatttct
      781 tcactgctct gcgacatctc tctgagctca tcttgtactg ttcttgaagc gaaaagagtc
      841 tgaacctcct gcggcatggc ggcaacattg ccgttgattt ttattttacc gctgtagggc
      901 ttttcaattc cgagaataat tttaaaaaga gttgttttgc ccgctccgtt tccgccgagc
      961 aaagcgaata tgcagccctg cggaattttc aggttcattg agtgtaaaat atcatcggaa
     1021 tctttttcgt atttaaacga aatatttttt aattctattg catttttttc actgaaaact
     1081 atactttttc tttcaacttt tttgcaaaaa atattttgac tcccgagcca atttcttccg
     1141 tcacgcacac aaacgggagc ttcacctttt tcatttgtgg aagcaaaaat cttcatagcc
     1201 gcaggcaagc tcagcttaac gaactcgggt aaattgcttg cgatttctcc gatttttttc
     1261 ggctcaccat cggcaataat ttttccgtta tacattacaa caattctgtc ggcaaaagaa
     1321 aaaatatttt caagtctgtg ctcggtcagg attacagtga cgccgagatc tttattgatt
     1381 ttattgacgg tttcgaggaa gcgctcgccg gcgatcgggt caagacgtga ggtcggctcg
     1441 tcaagaatca aaatgtcggg gtgcattgcc atcacggcgg caagatttaa tatttgtttc
     1501 tgaccgccgg aaagctcggc tgttttttta tcgaaccaat cgtcgatgcc gaagtatgaa
     1561 gccatctcgg caactctcag acggatttct gccgtttcca ttccgaggtt ttcaagagca
     1621 aatgcaagct cgtgccacac cttgtccgtg acaatttgac tctcgggatt ttgcataaca
     1681 aagccgatct tttcagactg cgttctgagg tctgtttcgt caagcttcgt gccgttaaaa
     1741 tatatttcgc catcgcgctc accctttggt gcaattgcgg gcttgagttg acgaagcagg
     1801 gtcgtttttc cacagccgct ttttccgcag agaattataa actcgccctc gttgattcta
     1861 aggttaattt tttcaagact gtttttattg ccgagagaat atcggaagct taaattttcg
     1921 attgtaatct gtgccatttg atatcctcct ttaaatcaat tgttagcggt aaaaagcaaa
     1981 gcaaagtgaa agcaatgagt gaaactattg atagaaaacc gaacggattt attgtcaaat
     2041 tcagttcgtt tatgatgtag gtcgtgccga tttcggacgg ttgatttatt atcacatagg
     2101 gattgtaaat gcagtaagcc gaattgctga caagaccgaa tataattaaa gcgtcgaaca
     2161 gcacaagcaa aataagcgca agtgtgtcgc ccgtatgcca ttgaaatctt gagtaatagg
     2221 ttctgccttt gagaccgtag cctctcgctt tcatggagtc ggcagtttcg attccattct
     2281 cgaagctcca cgaaacaagt attccgatat ttttgaaagt gtttttcatc ttgcagatga
     2341 agctgtccgt tccgtttagt cccatacatt ttcttgcttg cgatatttct ttatagcgtc
     2401 ggcgatagag aggaacaaat cgaagtatca ttgaaacaac aagtgcaccc tttggtaaaa
     2461 tcctgccgaa gatgtgcatg aacttgtcct ctgtcacaac ctcgttgcag caaaagaacc
     2521 attggattac cgaaacgact gtaattccag aaacgatgcc catgacgagg gcctcaaatg
     2581 taaaattgtt gccggagggc aaaataaaaa gagttgtaac tcnnnnnnnn nncaataatg
     2641 cttatgaaaa ataatacatt aacaagaggg tgatatcctt taaatcctga gctcataatt
     2701 taaccgaaaa gctttttgaa aaagctgaca atagcattga ttattctttt gaaaaatgat
     2761 atttttcccg ttttgttgcg aagggcgaag atatagcctg agtcgttctt atagaagatc
     2821 gttccgtttt cgccggcaac aattgagctg atacaatagt tgcttttttc ccctgtagga
     2881 gtgtaaagct cctgcatatc cgctacggtc tgaccggcgg aatccttgaa cacggtcact
     2941 ccgccggggg cacagttata agtgatataa atatttacct tgcccgtatc cttgagataa
     3001 gcgtcactta aaagaaattt accctgcgga tagccttttg tctgcgccga atagataacg
     3061 gccagcctgt tagcatcaat taccttgaaa tatcccgctc cgaagccttt gccctgaacg
     3121 ccgagataaa gcctgccgtt atagatgagc ggcgttgaag tcgaggctcc cccgagcgag
     3181 agacgcttaa ccgaggaatc gttaaaagtt ccgtttgcgt taagcttcac actgtacaga
     3241 tatcctgcct tggtagtgaa ataaagcgtt ccgttttgat aagtcatgga tgaacgttgg
     3301 tcaccgacta tcgggagagt atctacaagc tttccggtct ttttgttgat ggaaaccgcc
     3361 tttgaatttt tgttggcgta aatcgttccg tcgtcgcagc cgaaaacaac attgtcaccg
     3421 actgcggcac aaccnnnnnn nnnnaccgat tatagcatat ttatcggtaa catatgcacc
     3481 tgcccaatag aagccgccga ggcttgtgta agtccatgtt gccttctttg cttcatgggt
     3541 ttcttttttg ttttcatcgg taacgtttat gcaaacataa tttgcttttc tgtcctcgtc
     3601 gttccaaaat cccgtgtaaa tacagccgtc gctgtaagtt atcggggtca gcgattgacc
     3661 gccgagcgga tcggaataga cccacagtga tttcattgtt ttatagtcaa atgcttgaat
     3721 ttttgcgttg tcgagagggc agtagataac accgtttgca taaacaggtg gtgtatagct
     3781 gtagctcggc atatcgacca tatcggtaga tttgaccgtt ttgccgttct tgacattgag
     3841 cttcagaagt ttcttgttgc tcataactat gannnnnnnn nnagtgtatc gccgacaacg
     3901 acctgagggc tcggtgcgtc tttatatgat gagccgagct tgacgtccca tgcaagctcc
     3961 gtgttcgcag cggatacggg agttttgcat gaaacgatct gggaatcgga aacatataat
     4021 gttgacgcaa atacctgaac gggagaaacg agaagcagca cacaaagaac ggtgcaaaga
     4081 attcttttaa gcttcattta tcagcacatc ccatcaaaaa ttttgttaaa gatttttaca
     4141 atgaaattat aaatggcctt aaacatacga attatgaaaa tatccttctt tgatgttacg
     4201 gtcacatttg aataggcgac aaccgtcgga gcctcctcac tgtcggagct tctgacggca
     4261 acgagggtat attcgccttc ctcgctgaac ttgatttttg catttccttt tttgtttgtc
     4321 gttgcaagtt ttttaagctc tccgttttca tatagataga tgtcaacgtt cttgagattg
     4381 gtcatgtaat ttgctctgac cgtatcaata tcgttgagac cgtgctccat gagcgagtac
     4441 cccttgatgt ttaccttgag cttacttcct gccgaaacag tgtagctgtc agagtcaaac
     4501 tcggcataca tatcactgta gtatcttgta tcctgataga aataatatga aacgctgtcg
     4561 ccgtcaacga ttgctgcggt gtcgcaagcg tagcctgtcg gagtgccgta ggagggattg
     4621 atgatgccgt cgttgggcat tgttccgttg actgtaaatc cgcaagccga agtgtttctg
     4681 ccgaatgact tcataataaa gctgctgttc ataacaaggt agtttttggc ggtgcttctt
     4741 gtaaattcgt caccgtaata tgccttatga gcggctacta ttgcatccat aaccgttgcg
     4801 ccttcgacct tttgaccatt gtggtcaaat gaggctacct ggtagccgta agcccttgcg
     4861 atgcctgctt caacctcaag agtctcggga gccatgataa tcgtgccgtc ggaaacgctg
     4921 aacgttacgc tgactgtctc tgtgcttccc ttaggaagaa ccgtcacaac acactcggct
     4981 gtctgaccct tgggagaggt tgcggttatt gttgccgtgc cctcgttgat tgtataaacc
     5041 ttgccgtcat tgtcaacgta tgcaacgtct tcgtttgatg aagtccatgt tactcccttg
     5101 agatttgctt tgagctgagc cgactcgtat ttttgaattt caagagttga gtaggagagt
     5161 ttaaactcac tctcggggag ggcggagctg acgtcaagca tgaagatctg agcttcaccg
     5221 tcgcctgttt gaacaattac tcggacaaga ccctttgcgg gagcctcggc aaacattttt
     5281 tcttctgttc tctcattgtt gatataaaca tttgagttct cggcagttac tttaatgtca
     5341 acaccttttc cgagatcgtc aagctttgca aagttatttg aatcaaattc aacctttttt
     5401 ccgtttacgg tgatcgcgac gtcagacttg tcatttgtaa aatcttcatc ggaagcgagg
     5461 ttaacaccgt tgatttctgt tgacttttcg ccgaaaacac cgccgtcaat gaaggttgcg
     5521 gtctgaacct taatgtactt gacccagtca aggtgaacgg gcataccgct ctcgtcaacc
     5581 gcccatgaga tatcaaactg accgtcctta gcattctgct gcggtttttc aacgtatgga
     5641 ttaacgggaa tgtcactcat tttccagctc aaagcatcaa catatccgaa tgaggtaata
     5701 atgccgtttg ctgttgacgg ggtgaggttc tttctgagaa gaacgcccga aagtgtaagg
     5761 ctgttttcgt caaaatcaac cgttggatag tttgccttga gggggtaggg cgatcttgag
     5821 caaacgttgc cgctttcacc aaggctgtca acataggtcg nnnnnnnnnn tttttgatac
     5881 gtaacgctgt agttccagtt tgtctcgtcc tcatagtgct cgctgcctgc gagagcatac
     5941 catgttgagc cgtcctgaga aacccagacc tgtcccggtt cctgagttgt tgcggcggca
     6001 ttaaatgcat ttcccgaaat cataaaatca atgccgaaac gatgcctgtc actgttttct
     6061 atattcttat tgaattcata gataacataa ccgccgaagt tgccgagcga ggctgtgtag
     6121 gctttgcctg aaagtgtgtt ttcaatattt gctccgtagt acggattgtt tgtgtactgg
     6181 ccgggcgcta caaggtattc cttgatcttt gcatcaatcg ctccttcggc aaatgctgtt
     6241 accgaaatgc atgaaagtgc catgataatt gcaataaaaa ttgaaagtgt ctttttaagc
     6301 attgtttgat tctccttttc tgcgcgcttt tgagcgctgc cttttttcat agttgcgctg
     6361 tttgcgctcc tcttgctttg attctttaag tcccgctgct tcgagtgccc atggccaagg
     6421 cccgaaaaag ccttttattc ggttaacatc gtcctcggaa aaatcactct ttttcgggaa
     6481 gcgcttttct ttgctcgcaa cagctctgag taaatcaaga tactcttcct ttgtcnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnttc aatcacggta atggctgttg cgacggattt gcaccgaact tccctccgat
     6661 atgcttttta tttatgataa catttattat cggcaatgtc aataaagttg acatcctgaa
     6721 tttatattgt ataattaaac tatcatataa aaggcgggca gtgaaaagat gaaaaaaaat
     6781 aaaattactt ttgcggttat aattgcggcg gaaattgcgg caattgttat ttctcttgtc
     6841 gtttttaacg gattgttctc tttgtgggca gtctgtcttc ttgctgttga acttgctgcg
     6901 gccaccatgt tctttatcca tgccgaaagc tatgaaaatc agcttgagct tatactcgtc
     6961 ccgtttttta tgtctccgat tattgcaaca ttcataaaaa caaaatcgta tttaatcatt
     7021 gttccgattg cggcgatatt tatatttttg actgttgagg gaatacgtcg aagatttaaa
     7081 aaatgcgaaa taatattaaa tgaaacagac gataaggcag aaatatatgc cgataaatcc
     7141 gtgcttctta ttgttcctca tcaggacgat gatataaacc tgatgggcgg tatgctcgaa
     7201 gaatatgtca gatacaaaag tgatataaga atttgttttt acactaacgg agattttgaa
     7261 gttagtgcag aaacaaggct gaacgagagc ttgaaggtgg ctgaattcta cggaatatcc
     7321 gcagaaaata ttatttttat gggctatggc gaccgttgga atccggagga cggacacatt
     7381 tatacctcac cagagggaaa aaccgtaaac tcagcttccg gaaaaacaat gacatacgct
     7441 ttacccgatc atcccgcata caataacggc gcagagtata agcgtgagaa tgttgtcggc
     7501 gacatgaaag agattatcga agagttcaag ccgacagata tcttttgtgt cgattatgat
     7561 tgccacacgg atcacgttgc gtgttcgctt ttctttgagg aggctttgct taaggtcctt
     7621 gaagacaacg gcgattatcg cccaaatgtt tacaagggtt ttgcctatga aacggctttc
     7681 ttcggagaaa aggacttctt taaggaaaat atcgtttcaa ccgtgaatga aagacgtacc
     7741 gactatatgc agaagcgagt caattttaac tggcatgacc gcgtgcgctt tcctgtttct
     7801 gctaaggcgg caacaaggtt tatcgggaac agctcggttt atcgggcgct gtctttttat
     7861 aaatcccagg atgcaacgga ttatgccgaa tcaattataa acggagacaa agtcttttgg
     7921 cacaggagaa ccgactccgt tctttacagt gcaaagatta ccgtcagctc gggcaatggc
     7981 aaattgctga atgattttaa gctgtttgat acggatgctc tttatgattt tccgttgtca
     8041 aatataaagg gtatggatgt accgaattcc gccgaattcc ccgaaagcgg aatttggaag
     8101 cccgatgtga acgacgaaaa gaaggaaatc aaggttgaac tcggagaaag caagaatata
     8161 aaagtgattt atctttatga taatccttcg cgcaccgaca atataatcaa tgcagagatt
     8221 atttttgaca gcggcaaaac gatagaaacg ggtccgctta attcggacgg ctctgcaacc
     8281 tcggttgaaa caaatgcgga taatgtttcg tcgttcgtgg tcaaaataac agattgggac
     8341 ggagacgcac ccggacttac cgagcttgan nnnnnnnnng tttataaagc tgaccgatga
     8401 gtgtgagaat tttatttacg attatattat tgaatcgggc gataagacaa agctgtcatt
     8461 atattcatat aatgctcccg agttttcaac ggagaattat agagttgact gcgacaacaa
     8521 aaaatgcttg gcagctattg aaagtgaaat gattgttgtg gcttgcccta aaggtgaaag
     8581 ctgcatgatt accgtgacat caaaggacgg attttcggac agcgcaagag tatcgaaccc
     8641 aaagaataaa aagcttctta aaaaggcaat tgattttgac aaataccatc atcgtgtttt
     8701 gaaatctcac aaacaaaagg tttattacaa aaatatgctt cttcattttt atgacgagct
     8761 gatgtggaaa ataaaaagga caaaataaaa tgttcctcga aattactttc ggggaacgta
     8821 aaatttttca gtgtcaatgc cctcatgttt caaaagaaag atttttttat caatgccgcc
     8881 ggcatagcct gtcaggtttc cgcttgcacc gacaacacgg tggcagggaa ttataatcga
     8941 aatcggattg tgaccgacag cattaccgac tgcctgagcc gacatccttt caatgccgtt
     9001 gagatgagca atctcttttg caatttcgcc gtaggttgtt gtttcgccgt acgggatttc
     9061 acaaagaatt ttccatacag attgcctgaa ttctcctcct ttcggagcga gcttaagctc
     9121 ggatattttc ggcttttcgc cgttcatgta acggtcaagc cattccttgg ttttgcaaag
     9181 cgttatatca tcgtcggagt atataaatcc gtccttgcaa tccgagcgga aatatttctg
     9241 accctcgatc cataggccga cgagcttgtt atcttccgca gcaagtgtaa gcttacccaa
     9301 tgtccgacag ccgtattcgg tcaatttaat cacatgataa actcccttcg accgtattat
     9361 ataaactaat tatagcatat gtttttgcag ctttcaacgt tgacacaata attaaaataa
     9421 gttataattc taaaaaaagg aggcagaaaa atgtttgatg caattatgat ttttattctg
     9481 gatgtaattg atcttataaa ggtaataatc ggtattttcg gttaaccgtt gacaacgaga
     9541 atttttaatt attcaaagaa gcgcaatact tttttgattt ttatggggtg ttgaatttga
     9601 aagcaatcaa aaaaataatt gcaattattc ttgccgttgt actttgcata ggattcgtct
     9661 ctgtatcggc gagcgcaaag gaaagactta attatcttct tatcggagat tcaatagccg
     9721 annnnnnnnn ngccgagggc ttcggtgtta aaaaccccga cgaggcgtca tacggaaaaa
     9781 tcattgccga cactaacgga tataattata aaaacgatgc gattatgggc agaaacagcg
     9841 tcggacttct caatcatctg actaatgatg aggaatatat aaacgatgtt aagtgggcgg
     9901 atattatcag cgtttcaatc ggcggaaacg acttccttct tgatcatgcg gcgctgctca
     9961 ttattgaggg aattattttc aacgattact caaaatttga taagataggc gagacctttt
    10021 atgagaattt ttcaaagagc atggaaagaa ttcgtgagtt gaatccaaat gcgaaagtca
    10081 ttgttcagct cctttatacg tcatggacat ttgattttgc gagaaagcct tacaatcagg
    10141 catcgaagcg aatcaatgat gcgattataa aatatgctga nnnnnnnnnn gaatatttac
    10201 gttgttgaca caagcagaat ttttgacggc cgccgggact gcatttccac agatacaatt
    10261 caccccaatg cgcagggaaa catcgagctt gcaaaggcgg ttcaggcaaa gctttgtgaa
    10321 ataggtatgg caagcacaac cgagcttgtt cttcttacgc ccggaattga cagaaattat
    10381 ctcatagagt atttcggcac gcctctcggt cagataatta tttttgccgc aaatgttgcg
    10441 acaggaaatt tcaactttgc attttaattt aaaggtcatc ggcttaggtc ggtgaccttt
    10501 tcgggttgac atatatgtga cgcattgcta taatctaaat tgatttttat ttgatttcgg
    10561 agaagaaaat ggcagaaaaa aagtgtaata ataaatgtaa aataaaaatg atcgacagtg
    10621 aaagctcgta cggttttgtg aatgaattat ccgaaaaagg cggacgattt ttaacaacaa
    10681 cgtattctgt tttcagcggt attgagctga tttacaatga cgctcacata atgagnnnnn
    10741 nnnnnttgaa tcaaaagctc cgtcggggaa tgtgatagag ataaatcact gccgggaggg
    10801 cagaatggag tgtcattacc gtgacgagtt ctgctacgtt gcaccgggtg atcttgttat
    10861 cggacgcgcg gaggaaaaat ctcagacaac atattttccg ctcagtcatt atcacggcat
    10921 aacggtgcgt atcgacgttg accgtgcgcc gagctgcctt tcctgtttac ttgacgatgt
    10981 aaatgtcaag ccgcaggcga ttgccgaaaa attctgcgga gaaaaaagct gctttatcgc
    11041 ccgatccgat ccgtctgtcg aacatatttt ttccgagctt tattctgtgc ctgaggagat
    11101 aaaaaggggt tatttcaaaa taaagatact tgagcttctg ctgtttctca gtgtgcttga
    11161 tacgggcgaa aacgaaatcg acagcgttta ttcggcaacg caggttgaac ttgcaaagca
    11221 ggtaagcgta tatctgacga aaaacatgga taagcgaatt acccttgaac agattgcaag
    11281 acattttcat gtttcgggaa cgcatatcaa aaacattttt aagggagttt acggcgtttc
    11341 actctacgcc tacacccgcg cgataaaaat ggagtcggcg gcatatatgc tcgaatatac
    11401 ggacaggaca gttacggata ttgccggaga gcacggatac gataacagca gcaagttcgc
    11461 acgggccttc cgtgaggtta agggaatgac cccgagcgag tacaggtcaa aaaataaaaa
    11521 ataaaaatgt ccgattggag cttttttcta tccttttgga gcggaaaagg ctacattttg
    11581 catgtaaaat aatgtacgaa gaaaggcaat gcggatatga tttatgcata cgtattgcct
    11641 aaaaaaacac cgacgagtta gcagacgcta accgaatgaa cggaggcggt agaatgtcgg
    11701 aagaattact tgttaagcat tgctcgccga cccttgccgg aattaaaacc ggcaatctgt
    11761 tttccgtgaa ttttgacaat gcggatgaat tgagagcatg cgtgcgcgat tggaacagaa
    11821 tgctttcgcc gaagggtctg cggattatgc cgatgaggtt caacgacggc cgcgcgttga
    11881 tttatgttta ccgtccctcc agactttcaa aggatttgag tgaaagcctg gcggaggata
    11941 tgcttgattc tttcggttac ggcggcaaaa cgcccgaacg ctgtgtaagt gagcttatga
    12001 agcggctgag aaaaaacgaa gcgtttccgc acgagatagg tttattcctc ggatatccgc
    12061 cggaggatgt taagggattt attgagaata aagcctgcgg ctgtaaatgc aagggctgct
    12121 ggaaggttta cggagacgaa gcggcagcaa gaaaaatctt tgcgagatat aagaaatgta
    12181 ctgcgattta ttgcgagctt ttcaaaaacg gcagaagtct tgataagctc acggttgcag
    12241 cataaatata agaaaggttt gatgaaaaat gagtaaaata gcagtagtat attggagcgg
    12301 cacaggtaac acagaggcaa tggcaggtgc agttgccgac ggcgcgaaag aaaagggtgc
    12361 agacgtttcc gtgtttacgg catccgagtt cagcggctcc atgatggatg aatttgacgc
    12421 ggttgctttc ggctgcccgt caatgggtgc ggaacagctt gaggacagcg agttcgaacc
    12481 tatgttcagc gattgcgagt ccaaactcag cggcaaaaga attgcccttt tcggctcata
    12541 cggctggggc gacggtgaat ggatgcgtaa ctgggcagag acctgcaagg atgacggcgc
    12601 agtaatggcg tgtgacttcg tgatttgcaa cgaagctccc gatgatgacg ctctcgacga
    12661 ttgcaaggca ctcggcgaag cattgacttg acagtacatc tccgatatgt aattgataat
    12721 ctcgattact aaaagcgcag gcatgttcaa tgtctgcctc cctcaccgtc acaaaagcgc
    12781 cgcacttcgt ttttgtgtgt tcctcttcct tgagaaaagc ggacggtgca cctgcgcttt
    12841 cggagattta ttatatttgt tatatatagc ggttatcaac ccttccgctt attatataaa
    12901 ctctttgctg aatagaaacg gcggctcact taggtgggtc gccgtttcgt tatacttata
    12961 aagctttgca aattatcgca aaacaccgcc gaacagaatc cctgctcggc ggcgggaatg
    13021 gtattttaac ttaatattta tcttttggga tacgggtttt gctgagcggt tctgccgaga
    13081 atataatcgg tggaggtctc gaggatatcg gcaagctcgc aaagcatttc aagcttcatg
    13141 gtccttctcc cgctctcata catcgaatac attgtttgac tgattccgaa atgcttagcc
    13201 atgtcaagct gtgtcaaatg tttatcacgt ctgagctctt ttaaaatgtc tgtgtaaact
    13261 gccataacaa cctccaaata ttacaaatgc tgttattgac aattagaaca cattttgata
    13321 taataatgtt gaaatattac atatgttgta atatcatata gcttgattgc tgcgaaacaa
    13381 aaacaccgcc gaacagattt tcactgctcg gcggtagtat gtattattta tttttatcat
    13441 tgctcagacc gagcaggtaa tctgtggaaa cattgtagta tttcgcgagt gtgataatgt
    13501 gatgactcgg aatgtccctg acaccgcgtt cgtatgcact gtaatattga tatgttgtgt
    13561 tcaatacatc ggcaatatcc ttttgagttt tatcgttatc ctcacgaata tctttcagtc
    13621 tgggatagta gtattcttgc ataaaattat caccgattct ttgtatattt gactgaatta
    13681 tatcatatat ggttagctta tgttgaaatt taatcatata tggtttataa tactattgca
    13741 acacattttg ttgcagttgg tattggcata tacacatcag tttttacaag ccctcagtca
    13801 cgacattttt gtgacagctc tcctacagga gcacagagat aaaaattttg actttttatt
    13861 tatataaatc gcatggcttg attgctgcga aacaaaaaca ccgccgaaca gaatccctgc
    13921 tcggcggcgt tttgctttat ttggttttat tctgctgcct cggcggagtc aaatacaaat
    13981 ccgtcatcgg tatgcttgca gatatacttg ttgcctgagg ttattctgcc gtcaagcatc
    14041 tcctcgctga gcttatcctc aatctccgac tgaatagcac gtctgagcgg tcttgcaccg
    14101 taaaccgggt caaagccctc atcggcaatc ttttcaattg cgctgtcgtc aaagctaagc
    14161 tcaatgccca tgtcgtgaac acggttcttg agtgttttga gcattctgcg tgcaatttcc
    14221 ttaatgtcgt ccttattgag ctgctcaaat acgatgatat catcaaccct gttaaggaac
    14281 tcgggacgga agcacttctt caattcgccc atgactgcgt ctttaatatc actctggctc
    14341 attgtgccgt tgccttcctc gcccttgaag ccgagtgcgg cgttgccggc gtttgtgatg
    14401 agctttgcac cgacgtttga agtcatgatg atgatgcagt tcttgaagtc aacctttctg
    14461 ccctgcgaat cggtgagaac accgtcgtca aggatctgaa ggagcatgtt gaatacatcg
    14521 gggtgagcct tttcaatctc gtcgaaaaga actacgctgt agggtttacg gcgaaccttt
    14581 tctgtgagct gaccgccctc gtcatatcc
//
LOCUS       DAWBSW010000030        14006 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_942_length_14006_cov_2.620959, whole genome shotgun sequence.
ACCESSION   DAWBSW010000030 DAWBSW010000000
VERSION     DAWBSW010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_14006_cov_2.620959"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    210..262
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2057..2066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2448..2457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4053..4062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5999..6008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6324..6333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6753..6762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9777..9786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12168..12177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3598 a         3458 c         2740 g         4077 t
ORIGIN      
        1 ccctctgctt gtaaggcaga tgctctccca gctgagctat gctcccattc gccatatttt
       61 cttattgtta cgcagcctta ttggctgaga acgtatgtta ttataccaca gaatttgata
      121 attgcaagtg tttttttgaa aaaacttcaa ttattttatc atctctctga attcgtcctc
      181 gctgatgacc tttatgccga ggtcattcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nntcaaaaca aaggtcttgc cggcaaagcg catatcctta
      301 acctctttaa gcgatttcat gttaagtcct gccgccatga gtttatctat aagttctatg
      361 ctctcgggca tattaaaata ctcaacaacg ctctttgcca taattgcgcc gaagccgtca
      421 atcgtcatta tctcatcaaa gcttgcattc atcagcgcag tcattgtgcc gaaccgctcg
      481 gatataagct tcgccgcctt ctgtcctaca tggcggattc ccagcgcaaa aatgagcttt
      541 gaaaggtcgt ttttctttga ctcattgatt gccgcaacaa gattatttgc cgactgctcc
      601 gccattcttt cgagtggaac gatctgttcc tgcttcaacg catagatatc cgcaggtgtc
      661 tttatcaatc ccgaattaac aaaaagctcg atattgctct caccgagtcc ctcaatatcc
      721 attgcgtcac gggaacagaa atgaataaga tttcgcaaaa gctgagcggg acaatcagta
      781 ttaaggcaac gaaccgccgc ctcaccctcc tcgtggacga cctttgcgcc gcatgacggg
      841 caaactgacg gcatgacgta tggcaacgat gcacccgaat gctcaacaac gttcagcact
      901 tccggaataa tgtcgcccgc cttacgtata gttacagtgt cgcctatgcg gatatccttt
      961 tcggaaataa agtcctgatt atgaagcgtt gcacggctta taagcgaacc tgcgaccatg
     1021 acaggttcaa acacagccgt cggggtcaga acgcctgtcc tgccgacatt tatttcaatg
     1081 tcaacgacct tcgtctgctt ctcctcgggc ggatatttaa aggcaattgc ccatttcgga
     1141 aactttgatg tgcttcccaa ggcttcacgc tgctcaaagc tgtcaacttt tataaccgca
     1201 ccgtcaatat caaacggcaa tgttcctctt atatcgccta ttctctcaac ctctttaatt
     1261 gcatcctcaa tattcgtgca cggagtatag aatggaatcg ttttgaaacc gagctctttg
     1321 atataatcaa gcgactgctt gtgccctgtc agcaccttgc cgtttatacg ttgaatattg
     1381 aatacgaaaa tatcaagatg gcgcttcgcc gtaatcttcg gatctttctg tctgagcgag
     1441 cctgccgccg cattgcgcgg atttttgaac ggcttctcct cgttattctc ctgctgctca
     1501 acaagcttca taaagacctc acgcggcata taaacctcgc ctctgacctc tatctcgtca
     1561 atctcccttt taaggcgcag aggtatagag ccgaccgtgc agagatttgc gctgacgtcc
     1621 tcgccgataa caccgtcgcc acgggttgag cctcttttaa aaactccgtt ttcatattca
     1681 agcgaaacgg aaagtccgtc tattttcggc tcaacaacat aggttgcgtt cggctcgacc
     1741 tctttaactc tcttgtcaaa agctctcagt tcgtcaaggc tgaaggcatc ctgcaagctc
     1801 tccattcgca cggtatgctc aacgggcgta aactgaccgt cagcactgcc gccgacacgc
     1861 cttgtcggag aatcgggcgt gacaagctcg ggaaacttat cctcaatatc agaaagctcc
     1921 ctcatcatct tgtcgtattc aaaatcctca atctcgggat tatcctcgac atagtatttg
     1981 tggctgtggt agttaaggag atccctcagc tcctttgcgc gtttagttga tctttcaaat
     2041 tcactcataa cagcacnnnn nnnnnnattt tagactttta accttttaaa ggctgcgttt
     2101 tattttaata tcatttaaag ccgaatatca taatttcaat attctttctt gcctctttat
     2161 cccgttatac ccgcagcaca ttctcttgat tattatacca aattcaagca cgagttacaa
     2221 cttttatcga ataatttccg caaaagtttg cggtgtaatt ccgacaacta tgctttctgc
     2281 tatgcacacg ttggttttat acggcacaac ggttacattg cccgaatgaa taacataagt
     2341 attcacagtt atattcatca taattcgatg atgtgtctga ttaactcccg ccgccgtgaa
     2401 ttcgttttca aaatcggtcg ttgttattcc cgtcataacg aatatcannn nnnnnnnatt
     2461 ttcggtcctc tgccgttgat aagctgttcg ctgaaaagca cgctgaacgg aatttttata
     2521 acatatgatt tcatttgatt tatgtgcttg tcgatctcgt cgcctatatt gttacctatt
     2581 ttgttaagat aatatgcgtc ggtcgtaacg gatacgatat ttccgttttt gtctctgccg
     2641 aattttatca gatcggcata cctcaccttg tcggtatcaa gggaattctc aattcctgcc
     2701 gtcgtcgccg cagaagccgc attcctcgcc gaataattaa caagctcttt tcggatgttc
     2761 gatatccgaa tttccgccat aataagcaaa gccccgatcg gaacgattag aagcatcgca
     2821 acgatcgggg aaaattttct ttttcgtttc aattatctca ccgcctattt tatactatgc
     2881 aaaaaggcgt cggtgagaaa ctaagcaatc aatatattaa atcattgtgc ctaaaatggc
     2941 aataataagc ggcgctaaga aagtaagatt gaaatagaag cccgaaacat cgaacatatc
     3001 gtccgaaatg tccgcggtta tttcagacac attgaacggc atcatatctg ccgaagaaat
     3061 agtctgactt gcatcttcaa ggtctgcggc atacatcttg aagcctacca aagactcgcc
     3121 gcgataataa aaatcaacgt aagagtcaag cattgcagca actccgtcgc cgttgtctcc
     3181 cgcaagctcg gcgtatttaa gaagttcttc ctttgtgata tcttcgggat tcatgccctc
     3241 aggcaaggtg ataatgccct tttcaaccgc cttttttgcg gttgcggcaa cgtcgggctc
     3301 aaaatgctca acgctgacct gtccgtaagg ctcaacctcc ttatcctcaa cgcttgcaag
     3361 cttcatgcaa ggcataatgt cattgccaag gacttcaata acggcaaaaa gaagctcacc
     3421 gtagtccgaa gcactgccgt cataatcaag aaaatccgtt attctcatct taaccttcca
     3481 tgaaggtata tatatccaaa tgtctttttt atccataatc agccttgcat tcacgccgaa
     3541 aacagttccc gtgcccgcaa ttttaagctc gtttttgccg tttttgtccg taaaaatctt
     3601 agccgcaagc ttaacatcgg ttattttaat tttaccgaaa tcaagctcac cgtcgggaac
     3661 ggacacgcct acggattttg tttcattcag ctttttaaga aaatcctcgg atctgcttcc
     3721 ctcggcaaaa gcattgatgc caagaagcgc ggaaacaaga attacaactg ttaaaagaac
     3781 actgataaat tttttcataa atattcctcc ttgatttaat gataatacac gcatgcatca
     3841 ttgtcaagtt tttgcgtaaa gaaaaaaatc agtctcccat ttaagagaga ctgacaaaat
     3901 tctaatatca atcaaggaaa tccttgagct tcttgcttct gctcgggtga cggagctttc
     3961 tgagagcctt ggcctcaatc tgacggatac gctcacgggt aacatcaaat tccttgccga
     4021 cctcctcaag cgtgtgcgga tgtccgccct cannnnnnnn nnaatgaagt cgccaaggtg
     4081 gctgtcctcc tcctcaccga tgggtgtttc aagcgaaacg ggttcctgag ccacgcgaag
     4141 aatttcacga acctttgaaa caggcattga aagctcctgt gcgatctcct ctgcgctcgg
     4201 atctctgccg ttcttatgga gaagctgact gttggtcttt ttaaccttat tgatagtctc
     4261 aaccatatga acggggatac gaatcgttct cgcctgatcg gcaatggcac gggttatagc
     4321 ctgtctgatc caccaagtag cataggttga aaacttaaag ccctttgtat agtcgaactt
     4381 gtcaaccgcc ttgataagtc cgaggttacc ctcctggata aggtcaagga agtgcatacc
     4441 tctgccgaca tatctctttg caattgaaac aacaagacgg aggtttgcct cggcaagacg
     4501 tttcttggcg gcggcatcgt tatcgtttat tctcattgca aggtcaattt cttcctcggc
     4561 cgtaagaagc ggaatacgtc cgatctcttt aagataaacc ttgacaggat cgtctgctcc
     4621 ctgattttta tcgtcaacat cttcggttgc aagctccgcc gacttgggca tatccatatt
     4681 aaaatcaaca ttttcaagat caacgtttga aaggtcgtca acaagctcga tattctgatt
     4741 ttcaatcgtt tcatagagct tgtccagatc ctcaaggtca aggtcaatat cgagaattgc
     4801 ctggtcgata tcctgagttg tgagcttgcc gttcgccttg cctttttcaa taaggttttt
     4861 gagaacggtt ttcttgtcca aaggtgctgt cattgcattg ttttccatag cgttactcct
     4921 tatttaactt ccgtttggat ttttgaaaag attcctgaat tcatcgtcgc tcaactgaga
     4981 aacatcggaa acagacatct tatttttttc tttttcaaga acttttatac aatcatcaca
     5041 ttccgtgacg gtgttactta gctgatcggt cattgacgcg aagcgtgcga ttcttcccat
     5101 ctcctccgga gtgaactccg cagaaatcag cgaaagctcc gtttccctgc cctcgtcaag
     5161 tcggcttgca agcgcatgcc aaacgcgacg attgaaatcg gtaacaaata tatcctcgct
     5221 gaccttatcc ttgatttttc tgtaaaaatc gggattttgc attatcgtgg caatgagcgt
     5281 ttcctccgct tttgcggcct tgaggtgctt tgaccgctcg ggattcaccg tatccttcgg
     5341 catgaccgta tcacggacga tctcgttgaa ctgctccttg ctttgcactt tcgtgcgtcg
     5401 tttatatgca cgatttatct cacggattat ggcatccttg ctcaccgaca tttcatcggc
     5461 aagcttcgac gcatacatat cctgctcaat cggattaccc tttgaaagga tatcggcaac
     5521 ggctgtcaga aacttcgact tgccgtcgat cgactcaacg tcgtattttt cacgctcacg
     5581 caaaatgccg tattctatat catttgcggc accgtcgaga agattctgaa acttctctct
     5641 gccgtaattt ttaataatct catcgggatc cttgccgcct gtcagcttca ggactcttat
     5701 tttcagaccc gtctgtccga aaattccgat tgcacgcttg gttgccttct gacctgcctc
     5761 atcggaatca taggaaagaa taacctcgtc ggtatatctt gcgatcaatc tcgcctgttc
     5821 ctgcgtaagt gcagttccga gacaggcgat ggaattagta aatcctgcct gatgaagcgc
     5881 gataacatcc atatagccct cgcagactat cagactgctc gggttgccgt tcttggcaaa
     5941 attcagcgca aatacgccgt tgcttttctt gtaaacaagc gtgtccgatg tgttgacgnn
     6001 nnnnnnnnga cgtacttagg ctttgagtcg tcaagcacac gtccgccgaa agctataaca
     6061 tttcctctta ggtcgattat cggcaccatg gcacggtttc tgaaattatc ataatagctc
     6121 actctgccgt ttttttcgct ctttttaaca aggttggcgt cataaagttc ctgatttgtg
     6181 aagccttttg agtgcatatg attacgcagt gcgtcccagg aatcgggagc aaaaccgagg
     6241 ccgaaacgct ttatcgtctg cattgaaagc cgtcgcttgc ctgcaaaata atcaagacct
     6301 accttgccct gcggcgacat catnnnnnnn nnnaaaatcc gctggcggtg ctttgcgatc
     6361 gtatcatcgt agccatcgtc gggcattctc attcctgccc tgtctgcgag ctgcttgacc
     6421 gcctcgacat aatcaagatt ttcgattcgt cgcataaatg ttattgcgtc gccgcctgcg
     6481 ccgcaaccga agcaatagaa cgactgactc tctggataaa cggtaaagga cggtgtcttt
     6541 tcattatgaa acgggcacaa tccctttggg tttctgcctc gttttgagag gttgacatat
     6601 tgagatataa ggctttcgat atcggttctt tcacgcaatt cattcagaaa ttcatcgctt
     6661 attgccataa tatcaacctc cttaattttt attttaaacc atcatgcacg aattgggcac
     6721 gaaaagcttt tcaaacgtgc taacggcaaa tcnnnnnnnn nntccgtcat gcctgcaaca
     6781 taatcgcaga cggcacgctc gacgccctct ttttcactta tttttatgta aagctcgggc
     6841 atctcgtcag gatgattcaa atagtattca taaagccact ttatcatctc gattgccttt
     6901 gtctcttcgc tcttgcaagc gggattagtg taaactctct caaacataaa cgaatgaaga
     6961 gttgcgaacg catcggcgca gtcttcgccg agaattatat cattcctgct gtttttcaca
     7021 accgatttaa caagcttatc tatacgctgc ccctttgaat agccgagcgc atcacgaata
     7081 tcttcctctt tcatcactcc ggcatgaacc gcatcatcaa tatcatgatt tatatatgcg
     7141 attctgtcgg caatacgaac aattctgccc tccagcgttg ctgcttcctc accgtttgtg
     7201 tggcggtcaa tgccgtttct gacctcccat gtgagattca aacctttgcc ctctttttca
     7261 atgacttcaa ccactctgac gctctgctca tagtgacgga aaccgccgta gcaaacgctg
     7321 ttaagtgcac gctcacccgc atggccgaac ggggtatgtc cgaggtcatg gccgagcgca
     7381 atagcttccg tcagtgtctc gttaagtccg agacaaacgg caattgtcct cgcaatctga
     7441 gcgacctcaa gggtgtgtgt cagtcgggtt ctgtaataat cgccctgcgg cgaaagaaaa
     7501 acctgggttt tatgcttgag acggcgaaaa gagttgcaat gtattacacg gtcacggtca
     7561 tgctgaaagc acgttctcag ctcggtttcg ggttcaaata cctttctgcc tttgctcgaa
     7621 tccgagaaac aggcgttact gctcagaata cgatgctcgt tttcacaaag tctttctctg
     7681 accgtttttt ccatacaaaa cacctcccga aggcttctca ataattatat actccgtttt
     7741 tcgccctttt cctcttttta ttttaaaaat tttaaggcat tgcctcaaaa acctcgccga
     7801 tttcttcgct tttgaacttt ccgaagctca aatcaacaag ttttttttca aaattatcga
     7861 catcatcggt cggcattttg aatgtaaccg tcactgtgtc ggcaaaatcg gcattatcaa
     7921 ttgccccgcc gcactcgggt ataagcgaat tgagcctgcc gtaaaagttg taatcacagc
     7981 agaccttcag caccttgcag agcttcattg ttataattcc gcctgcgtca acggcaatct
     8041 tggcggtatg ggaatatgcc ctgacaagtc cgccggtgcc gagaagcgtg ccgccgaaat
     8101 atcgggttgc aacaacaaca cagtcggtca gattctcttt aagaagaaca tcaagcacgg
     8161 gtatccctgc cgtgccctgc ggctcgccgt catcggaata ccgtcgagtc tgaccgtccc
     8221 tgaggacgta ggcgtagaca ttgtgggtcg cgtcccaatg cttcgatttt atctcattta
     8281 taaaggtgac cgcctcatcc tgagttgtga cgggcttaat atagccgata aatcttgatt
     8341 ttttttcaac gaattcgtcg ctcgacgctt tcgccacggt tctgtactcc aaagcgatcc
     8401 ccctttcact cttgttgatt taaaaaaaga cggtgttaaa cacaccgcct tttctctcat
     8461 cattatttag acaaattgaa acgcttattg aagcggtcaa cacgtccgcc ggtatcaaca
     8521 agcttctgct tacctgtaaa gaacgggtgg cattttgagc aaatatcaac acggagatct
     8581 ttctttgttg aacaagtctt tatctcatta ccgcaagcac aacgaacaac tgtctcttcg
     8641 tacttaggat gaattccttc cttcatgtgt ttcacctctt tcgatatatt cacgcaacag
     8701 ctattgtatc acaggttttg taaaaatgca atagctttta aaaatatttt taaaaaaaca
     8761 gaccccgtct gctgacagga tccgtgaaag taaaacagcc gttacaaaac gactgttcaa
     8821 tgatgtcggc gtcgccttat cttcccgggc ggctgcccgc caagtatttt cagcgctgaa
     8881 gagcttaact accgtgttcg ggatgggaac gggtggaccc tcttcgccat cgacaccgac
     8941 tccgctggga actattgttc ccaaacgact tgcttattat ataccgtgaa cgtcaatttg
     9001 tcaagcattt ttttaaattt tttacttttt ttcgataact ttttcaattt caccgacata
     9061 catatagtga taatcccctg aggcatagtt tttctcaatt gttttatcaa ggaatccgga
     9121 attatccaag ggctgaaccg caattttttt acatttaatt atcagtctcg cctctgccgg
     9181 ataaacggct gcgccgtcgg ttgcaagagt tagacccgct tcctttatct tgttacagtc
     9241 tctgccggaa attctgccgc aaaggcgaag catatcctta tattcgggat caaaaaagct
     9301 gatagtgaaa taatcctgct catcaataaa cttctttgta tatctctgcg gacgtataaa
     9361 tataaaagcg acgtctctgc cccaaagctc acctattcct ccccaactga cggtcagcgt
     9421 gttccaatct tcgggagttc ctgcgctgac aagcgcccag tcatcggcaa tcagcttaac
     9481 gagattttct tttaattctt taattgtcat agtaccacct catagagtaa tttatgcagt
     9541 ttttcttttt atcattccga cgagtgagaa tattccgaac atcatcgcat tcacggcaac
     9601 aacgctcgca cctgccggca ttgaaaacgc aaacgaaaca aacaaaccga taaagaagca
     9661 tataaccgaa ataacggccg aaacggcaac aactttttta aacgttttgc aaactcgcat
     9721 tgccgtcagc gccggaaaaa ttacaaggct cgaaatcagc attgctccca ttatccnnnn
     9781 nnnnnnccat tatcctcatg ccgataacaa ccgttattgc cgtcagcaag gctatcagcg
     9841 tgttgtacat tcccgttttt gttcctgttg ccgtcgcaaa gctttcatca aatgtaacag
     9901 aaaagatctt attataaaat acgcagtata gaatcagcac cgccgccgaa atcactatgc
     9961 ttattataac atccgtactg ctcattgcaa gtatactgcc gaacatatat ccgtttacgt
    10021 cggaattaag acccgttgtc attgaggtta caaggattcc gatcgccaat gccgtgcttg
    10081 agatcaaagc tatcgccgca tcgccttgga tcttgctgtt gtcacttatc cttaaaagca
    10141 gaaatgcgga tattattaca acaggtacgg aaactgcaag cggcgcaaca ttcattgcaa
    10201 gagcaacgca gagggcgccg aagccgacat gcgaaagtcc gtcgcctatc attgaatatc
    10261 gctttaagac gagcgtaacc ccgagcaaag ctgcacagag ggcaaccagc acaccgacga
    10321 ttatcgccct tcgcataaat ccataggaaa acatctgaac gaacatatca agcatggctg
    10381 tcacctccga gaatcctttt tgccgccgct gtttttagat attcctcagt cgtgccgaag
    10441 aaatcgccgt catgctccat gcaaagaatt ttgtttgcgt actgaattac gctcttaacg
    10501 tcatgggtta ccataatcac cgtaacgccg tgttctttgt tgagatgact tatcagctta
    10561 tacatatcct ctgtcacaaa cgggtcaaga ccggttgtcg gttcgtcaag aagcaaaagc
    10621 ctgtccgttg cgcataatgc tcttgcgagc aaaaccctct gctgttgacc gcccgaaagc
    10681 tcattgaagg acttattctt tattgaattt atcttcataa tatccatatt ttcctgcgca
    10741 attcgcctgt ctgcagcagt ataaaagagc ttcccttttc cgctgagtct gccactgaga
    10801 acgacctctc tgactcctgc cggaaaatct ttttgcacct gcgttttttg cggcagatag
    10861 ccaattccgc ttttctttat tccgttaaat tcaacttttc cctcggagat tttctgcaag
    10921 ccgaggatac ctttcaaaag cgtgctttta ccggctccgt tttcgccgac gatacaaaga
    10981 aaatcgccct catcaacggt gaaactgaca ccgttgaccg ctttggtcga atcgtaattc
    11041 atgtgaatgt tttcgcattt caaaatctgc atcagttcag cgcctccttt aaattcctgt
    11101 aattttgccg catgagcgag agataagtct ctcccctctt catttcgtcc gaggaaacat
    11161 tatgacacga atgaagcaga agcatttttg cacccgtttc aattgaaacc gcccttgcaa
    11221 ttttcggctc ggtcagctcc tcatagaaaa caacaggcaa attatcagca tttatcttgt
    11281 caatgatctg cgccataacc gatgcactcg gctcggtatt gtcaacgcag gaatcaaatg
    11341 ccgccgtaaa ttcaatgccg aattcacgag taaaattctt cattgcaaac cgtgaccccg
    11401 aaacaatctc ccttcgcttt gagcttttaa caagccgtga aaaatcgtct ctgagcttca
    11461 atatttccgc agaatatgcc tttgcgtttt cgctgtaata atccgcattg tccgtgtccg
    11521 ctttgcccat ttcctttgca atgcagtcaa tcatttttgt tgccgtttca aggtctgtcc
    11581 agatgtgctc gtccgttcca ctctcatcgt gctcatcatg ttcttcgccg tgatgatggc
    11641 catggtcgtg atcgtggtcg tttatcacaa ttccaagctc atcggctata tcaatttttt
    11701 taacgctttc attttcaaca gcatccgtta tctgcgtgac ccatgtttcc gtgtgctcgc
    11761 tgacatagaa gaaaatatcc gaattttcga gttcaattat atccctcgat gtcggctcgt
    11821 acgaatgact ctcaactccc gtcggcaaaa gcatttttac ttccgccctg tcgcctgcaa
    11881 tccggcgggc aaaatcatac ggtgcaaaaa tcgagcagac aacgctcagc ctgtcactct
    11941 ttttcggcgc aatgtacgaa cagccgcaaa gcataagcat taaaacagcg agcaaaatcg
    12001 ccgtaatttt attcttcatc caatgcctcc ctgcatttat cgcacaatcc gtaaagaacg
    12061 gttttctcgg ggcttatcac aaagccgtgg tgctccaaaa catgctgtgc cagctcatca
    12121 agataaccgc actccacgtg aataagtctg ccgcagcctg agcatttnnn nnnnnnngcc
    12181 tatacacagt ggccttgccg acaggttgtc cgtccttcgc aagctctgaa ataatttcgt
    12241 cggcagtaaa atgtctgccc ggtttttttt gcaagagtga aaggatcaat ccgccctgct
    12301 tcgtgttata tgaattctcc atttaattcg ccttccgaat atgaaactga atttcaattt
    12361 catattgtac tactcttttt ttgatttgtc aacaccgctt gtcaatatga ttattgtatg
    12421 gtaaaataaa atgtaaacat attatatgag ttatacaagg atgagattat gaaaaaaatt
    12481 atttcactga tatcatgcac cgttttgctt ttctccctct cctcttgttc ggttgaaaaa
    12541 acgccgatgc ttgacaacag cgaccaaagc tattttgtgg atttctatac cgatgacgat
    12601 tacgtttata ttgaatgtgt gctgaatatc tacaacccga acaacaccga atcagaggta
    12661 aaaatctcgg cgattgataa cgaggacgtt gaaatcggtc ttttgaaaac ttcaaatctc
    12721 attgctgttg ataaagaaac ctcaaaggag acctttcggc taaaaagcgg agaaaacaaa
    12781 ataaccgttc tttttaaagg cgaatatgcc ggaatttatc aaataacttc ccgcgagctg
    12841 ccgagattca tttatatcag cgaaaactga acacgcaaaa gcgcaggcct gacggtctgc
    12901 gcttttttga taaaacaatt atttcttaat cagtttgccc aactgttcaa taataatttt
    12961 gacaatatta actattatgt ttttaataag cgtaaagaaa ttcgggcttt caatcgggtc
    13021 acccttatcc tcctcggtca taggcttgat tgtttcaaca ttatcttcat ttttctcata
    13081 aataagatat tgaggaagat ttttctcgct gaacaccgta agcggctgtt cgccatgcct
    13141 caaaatcctg taaatcaccg ggtacagtgc ccacggataa cagtcgtgat tgaggttttg
    13201 aacataccag gtcgtgtccg ggaaaagcgc tgttgaagcg tcaattgaaa ggtcgggaga
    13261 aatatactta tctgttccgt ttgcctttgc ctgtgcaata taatcatcgc tgagcttttt
    13321 gccgatagga gctgtcgttg tgcccggagc ctgctgctcg caagtggtaa gcatatcact
    13381 ctgaagatcc gcattataaa caatggggta aagctggaaa ccgtacttag caacgattga
    13441 tacatgcact ccgtcggctt ccatctgctt atacatagag gtaagcttag aaccgactgt
    13501 ttcgtaataa tgatttattt tttggataag tcctgcatat tcgtcctcaa caccagcaaa
    13561 gatatagtcc cttgcctctt cgaatctgtc ggcggaaacc atagcccaat aagcggggaa
    13621 cgtaccgtaa cagaccctca taacatcggg aataacgtat tttgcaactc ttgtgcccgt
    13681 cttgttgaaa taatccattg tcatgtcaag tccgttcaac ttgttgctga gtgtgattat
    13741 ctctttaaga agggtaaaaa gaagcgaatc accgagattc tgagttgcaa agtagtcaac
    13801 tccgtccgca tcaaaataaa gttctccgtt gaagagcgaa ttgtttacct ctgtgccgaa
    13861 gcagattgaa ttatagagaa caacatcgtc gacgtcctcc cagccgtatt ccgcaaggta
    13921 tgcggagaga attatggtgc caaggcatct tccgagaagt ttaacacggg aatgccctgt
    13981 taccttttta accgcctgaa tatatt
//
LOCUS       DAWBSW010000031        13723 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_971_length_13723_cov_2.973954, whole genome shotgun sequence.
ACCESSION   DAWBSW010000031 DAWBSW010000000
VERSION     DAWBSW010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13723)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_13723_cov_2.973954"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2793..2802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3403 a         3373 c         3008 g         3929 t
ORIGIN      
        1 ggttttatca atgctctcat ccgtaacgat cggagccgcc gcatcaaaaa agctgagcga
       61 accgccgcaa agctccttga ttttttcggc aagagcatcg gaagcaagcg gaccggcggc
      121 aatgataacg ttgccctcgg gaatatccgt aacctcacgg cactcaagct cgatgagcgg
      181 atgcgacttt attttttccg ttatcatacg ggaaaattca tcacggtcaa cagcgagtgc
      241 accgcctgcc ggaacgctgc atttttttgc acattcaacg cagatcgaac cgagtctttc
      301 catttcggct ttgagcagtc ctgccgccga gccgacacgc tgagccttga gcgagttgga
      361 gcagacaagc tctgcgagca tatctgtttt gtgtgcggga gaatgtttct taggcttcat
      421 ctcggctatt gtcacgggaa cgcctctgtt tgcgagcgcc catgccgctt caacgcctgc
      481 caggcctccg cccacaacaa gaactctgtt catttactga tcttcctttt tcctgctttt
      541 tctttctgcc ttgcgctcat agccgcagtt ctcgtcaagg caaactatca gtccgcctct
      601 gcgcttaaag agcgacttgc cgcatttcgg gcaggtttca tcggtcggct tatcccatgt
      661 cataaagtcg cactcgggcc agtttgagca gccgaagaat gggaagcccc tcttcgactt
      721 tctctcgatg acctcaccgc cgcacttcgg acattttgca ttggtctttg tgacaagcgg
      781 ctttgcgttc ttacactccg gatatccggg acaagcaagg aattttccgt aacggccgac
      841 cttgataacc atcattctgc cgcacttttc gcacggaata tccgtcacgt cctcgtcaag
      901 ttgaatcttg acgtccttca tctctgtctt aaccttttgc aggttcttgt caaattcatc
      961 atagaattca tggagcattg cgatgtaatc ttccttaccc tcgccgacct cgtcaagctt
     1021 ggtctccatg cctgccgtaa attttgtatt aactatcttt gaaaacttct ctttaagaag
     1081 ctttgttatc gcttcgccca gctccgtcgg ataaagctgc ttctgctgac gctttacata
     1141 ttcgcgcttg actatcgttg tgatgatagt tgcgtatgtg ctcggacgtc cgattccgtt
     1201 ctcttcaagg gctttgatga gagaagcttc ggtatatctc gccggcggct gagtgaaatg
     1261 ctgattgcct ttcaaatcct tgagcttgag cttatcgccc gtgttcagct cgggaagctt
     1321 gccctcggct tcggcatcct cgtcgtccgt gctctcctca tagaggaccg taaatccgtc
     1381 gaatttaatg ctgtagcctg cggctgtgaa gcggcaataa ccgtcaacgc cctccgttac
     1441 ggcgtctatc tccgccttaa cggtattctg aatacaggtt gccatcaggc ttgcaacaaa
     1501 gcgcttccaa ataagcgtgt agagcttata ctggtctgaa gtaagactgc ctctgactct
     1561 gtcgggagtg agtgccggat tggtcggtcg gatagcctcg tgaccgtcct gagcattgct
     1621 tcttgacttg aaatatctcg gcttttcggg aagatatttc tcaccgtatg tctcaagtat
     1681 aaagctgttc gtggcggctc ttgcctcctc ggaaatcctc aaagagtcgg ttctcatgta
     1741 tgtgataaga ccggttgttc ctattcctgc aatgtcaacg ccctcgtaaa gctcctgagc
     1801 ggcacgcatt gtacgctgtg cctggaagcc cattcggcgt gacgcctcct gctggagagt
     1861 tgaggtaata aacggcggag cgggattttt ctttcttgtg cctttcttga cggaggtaac
     1921 gctgtacttt gcattgtcaa gtcttgcaag atatgcgtcc gcctgctcct tattcgtaat
     1981 cttgaccttg cctgtttcgt cgccgtagaa gcttgcggaa aaagtctttc tgctcggagc
     2041 ggtgaacttc gcatcgagcg tccaatattc ctcggggaca aaagctctta tctcattttc
     2101 acggtcaacg atgagcctta cggcaaccga ctgaacacgg cctgccgaaa gaccgcggcg
     2161 aattttctgc gaaataaacg ggctgagctt gtagcccaca aggcggtcaa gaattcttct
     2221 cgcttgctga gcatttacaa gattcatgtc aattgaacgg ggatttttca ttccgttctc
     2281 gatgccggtt tttgtgattt cgttgaacgt aattctgttt gccgcgttca tatccagacc
     2341 gagcaaggtg gcaagatgcc atgagatagc ttctccctca cggtccgggt cggttgcgag
     2401 ataaacgccg tctgactcgg caaccatgtt tttaagctcc ttaacgagct tctccttgcc
     2461 cttgataacg gcatatttcg gggcaaagtc gttcttaatg tcaacgctaa gcttagccgc
     2521 cggcaggtca cggacatgac ccatcgaagc ggttacgtta tagccttggc cgagatattt
     2581 ttttactgtt ttaacctttg tgggtgactc cactattacc agtttggaca taaatgcatc
     2641 atcctattta agtttgtagt ttttgcctgc aagaggttca atatatccca gaatttcaag
     2701 ctccgtcatt gccgaaagga aatcaaccgt actgagcttc gttctgagga taagctcctc
     2761 cgcctgcatg gtttcttccg aaaaggcatc gannnnnnnn nncttatatt gttcggagcg
     2821 acagacttga caaagttctt tgccgcctgc ttttccgctt tttcttcctt ttttcttttc
     2881 tctgtcctga ccgtctcggg ctcggttgtg agaatagatt ctatcttgtc aatatcaagc
     2941 atatctggat atctctcggc gtattcctcg agaacgtcca ttgcattgaa aacaggcttt
     3001 gctccgtcgc gcaggagctt gtttgtgccc atattcatgc tcgatgttat atcgcccggc
     3061 acggcaaaaa tgtccctgcc ctgctcgata gcatgattaa cggttctcag cgatccgctt
     3121 ctctcattcg cttctataac gatcgttccg taggaaagac ccgagataat acggtttctg
     3181 aacgggaaat tgtgtccgaa agcgggagtt ctcggcggat attccgtaac aagtgcgccg
     3241 tggtgtgcga tctctcgtct gagcggctcg ttttcgggaa gataatccgt tccgaatccg
     3301 cagccgagaa cagcgaccgt attggcgccg acattaagcg ctcccgtgtg agccgcactg
     3361 tcaataccct ttgccgcgcc gcttattaca acagcgcccg aacggctcaa cgaagccgaa
     3421 agcgaccgtg caattctcac gccgatatcg ctcgcctctc ttgcgccgac cattgcaacc
     3481 gcaattttgt ttttcaaaca gtctaaatct ccgcgaacat aaagcacaag cggtaaatta
     3541 ggcagcttga aaagcataga cggataatta tcgtcgtcag gggttacaat tttaatttta
     3601 tatttctcac attccgcaac gattttttcg gcactatcaa gagaagttga tttgagcttg
     3661 tttatctgtc cgtttgtaaa tattcccgat atcttccatt catattcacc ggcgtaataa
     3721 agcttttcag cattgccgaa ataggaaact atttcatcgg acttgacgct cgccgaaatt
     3781 ccgagagcgt tctgcgccca cacccagtaa agcttattgc tcattctctc accatctccc
     3841 aaataagtat tgccgctgca gttcctgcat taagcgattc cgccttgccg cccatgggta
     3901 tcgtgaccct catcttgcag gcgttcatcg tttccgccgt tacgccgttg ccctcattgc
     3961 cgacaacgca gactattccg cccgccatat tgacctcggt tattttcacg ctgcttttat
     4021 ccggcgttga agcgagggtt agcataccgt ttttgtttgc ctcgtttatt gtctcaacag
     4081 cgctctcacg gctgattatg ttcatcctga gagccgagcc cattgaagct cttagcgcct
     4141 tgggattgta gatatcgcat ccgccgcaga ctataagccc gtcaattccg agagcctcgg
     4201 cggttctgct gattgctccc aaatttgagg gatcctgcac attttcaagc atcagatacc
     4261 gcccgtctgt attcagggca gcgccttgtg ccgatttttc ggctcgacaa atgcaaaaaa
     4321 tcccctgcgg attttgcgta tcggaaagct tggcggcaac ctcgtccgaa atgccgtaaa
     4381 cctcctccga tgcagaaatt atccgttcgc aatattctcc atacttttcc gaagccgtac
     4441 tgctgagaaa ggttctgtaa atttcaatgc cgttttcggc ggcatccgaa cagagtcttg
     4501 ctccctcaag gaaaaaggtt ttgctttccc gacgggtctt tgaactgtcg cgcacggcga
     4561 ctgcggatct tattttttcg ttggttttcg accttaaaat atccatgttc aaagctcctt
     4621 tcgggtataa ttattacaaa aacaagcgac aaacaggtat cgctaaaaag acaaattcac
     4681 gcccccttag caaaaaaggg gcgtgatttt tatttattta agtgctgcct tagccttctc
     4741 tgtaagggct gtgaatgcag caggatcagc gattgcgatc tctgaaagca tctttctgtt
     4801 gagtgtgatg cctgccttgg taaggccgtt catgaatgtt gagtagttca tgccgttgag
     4861 cttgcaagct gcggaaattc ttgtgatcca aaggcggcgg aaatctctct tcttctgctt
     4921 acgtcctaca taagcatact gacctgattt cataactgcc tgcttagctg tcttgaaaag
     4981 acttgacttt gagccgtagt agcccttagc catcttcatt actttatttc ttctcttacg
     5041 agacatggta gcgcctttaa tacgagccat ggtaacttac ctccaatttt aaatttaacg
     5101 atctgaatta tttgtacggg atcaaacgct tgatctgctt aacgttcgtt gtgtctgcaa
     5161 cgactgtctg gcggagacct ctcttacgct tggtcgtctt tttgttgagg atgtgagact
     5221 tgttagcatg tcctctgatg ggcttaccgt tcttagacat cttaaagcgc ttctttgcac
     5281 cgctgtgtgt tttaatttta ggcatggttt tttcctcctt atgaattact tggatgattt
     5341 ggctgcgatg aacatcagca ttgaacgtcc ctcaagctta gccggcttct cgacggtgcc
     5401 gtactcactg acggcctcgg cgaatttgct catggtgaca agtccgtttt cgggatgagc
     5461 catttcacgg cctctgaaac ggattgaaac cttaactcgg ttaccgcttt taaggaactt
     5521 gatggcttgg ttagcctttg tctcaaaatc gtgcgtatca atgttgagcg acagacgaac
     5581 ctctttgatc tcaataactc tctggttctt tttggcttcc ttttcacgct ttgcctgttc
     5641 aaagcgatac ttgccgtaat ccatgatctt acagacaggc ggcttggcct gcggagcgat
     5701 ctttacaagg tctgtgttct tttcatctgc gagacgcata gcctctgcaa tcggcataat
     5761 gccgagctga gttccgtccg aatcgatcac tctgacttcc ctgtcgcgga tctcttcgtt
     5821 gatttgagtt tctttcgtag cgatgttcaa gcacctccaa atgctcaata ataaaatcaa
     5881 agcacggatg ggaacgaacc tatccgtgcg cataaatcta ccaaaaaatc aggcaaacta
     5941 ttgaccgcac ggacgaaacc gttaaggtgg gaacgaacca ttccactttg ttgtgtgagt
     6001 attttatcac cctatccgcc ttttgtcaat agctttttac aatctttttt ctcttattcc
     6061 ttaataaata tattatcatt ctgtaaattt tgcaatacac aattgaattt cattttattt
     6121 cgtgttatta ttgttgaggc aataccgcac aaattcatta caagttagga ggaattccct
     6181 tggcagaaga taaagagaaa tccttaacgg acgaaaccct gccgaaagaa cccgaggaaa
     6241 cttccgcgag ctgggactgg gacgccgccg ttccgcagac cgacacaagc aacataacgc
     6301 ttgacgattt gcaggagacg gaggaaaagg tcgttgagca gcctgccgaa gaaccggagg
     6361 aaaatgaaac agagaaggaa gaagaagcgc cggcttcaaa tcccgacgat gacggacttt
     6421 gcattgtttg cggaaaggaa agaggcaaca gcccgagcga tctttactgc aatgcgtgcc
     6481 gtgcaaaatt tttgagaaca aactacggcg taggccatat tattctcgct tttgttatgg
     6541 ttatcgttgc cgcactcggc tatttcgttg cgacctcaac ggtcacggtg tcctcaaagc
     6601 tttacaaggc ggagcagtgc atttccgaaa agagatacaa cgacgctctc aacctttgca
     6661 gcgaggcttt tgacgcaacg gataagctca acagcggagt taacgccgtt ataaagggta
     6721 tcaattcaaa tgcaaccaat caggactggt tcacggaggg agaaaaggca acacacctta
     6781 tccttgaaac ctacgttcat aacatgacga tcaacgcttc cgaccgtcag acctttgttg
     6841 aagcggttga aaagtattat accgacggcg agcttgacca gccgagaaat gcgaacatca
     6901 agaagcttta caatgcaatc aagtccgttg atgctatcgc aacctcgctt gatgagaaaa
     6961 tgaacagctt tgtaacccag aaagaggacg gaacctatga cgttaaatat gacgaaggca
     7021 tcaagttctt tgattcctat aaggtagata ccgatatcga aaagggtctc atggaatatt
     7081 acaagttcat gcttgcttat tatgcaaaca aggacagcaa gaccgctctc ggctttattg
     7141 acaacgcata caagaagtcc ggcgagtacg gatatctgta tctccagaac tacatgggaa
     7201 tggcatggca gtataaggaa tacgacaagg tgcttgagct ttccaagatc gcaacggagc
     7261 tcaatgtcaa caatgcgtcg gcatattatt acgcagtcaa ggtttatatc gagaagaacg
     7321 actttgatac cgccgataag atgtgtgagg aaatgcgcaa ggcaaatccc gacggaattg
     7381 actattacag cacaaaggct gaggttctcc gccgtcaaaa caagctcgac gaggcgattg
     7441 aaatttgccg caagggtatt gttgcaggct cggattccga aatttaccgt cagcaggcta
     7501 ttgcatatct cctcaagagc gacaaggaga atgcgctcga agcaatcaag cagtcgtatg
     7561 aaattgcagc tcaaaaccag cagctttcaa tccagacggc aaacactgtt gctcttatct
     7621 gcttcatctg cgacgataca aagacgtatg aagaggttga agcgatgttc aagcagagca
     7681 gtatggaatt aagcgactct gttaagaagt gcatcaaggg cgacataacc tttgaggata
     7741 tattcatgaa aggatcgggt gagatatcat gacaatagtt tatgtaatta caaagtatat
     7801 caccgttatc ggctcgattc ttaaaggctt ctgggagcat cttttctgcc gaattctcgg
     7861 cgttcccgtt tccgacgccc gttatcttca ggcaacggag ctgtgcggtc acgttgagca
     7921 cgatttcaca aaaacaaagg cagtcacttt cttcattaac tatcttcccg gcatgatgaa
     7981 ccgtcttttc ggctatggca tggtcgtcgg cggatatctc ggtcttttct atatgaaagc
     8041 cggcacggac acggttgcgt tttggttctg cgttgcgctc tattatatcg gcgtttcgct
     8101 gctgtgcaac aacgctccgc tatatgagga cgctctcaat aactgggatc ttctctacgg
     8161 caagggtaaa aaggcaaaca tcttcctaaa gattttcgct tttcttccgt ccgtttactt
     8221 cattgtttcg gcatggctcg aaaagaacgg tctcagcctc cttatttgga tcgccctgac
     8281 cctcgtagga atttttgtac tttgatttga atgaataagt aaaaatcatt gctaagcaca
     8341 taaatacacc ccgaaagaaa tctttcgggg tgtattgtca tattctttta aaaacctctt
     8401 gaatcagttt ttgtgagaag ttgtctttgt cctttgacat ttctgtgagt tcggctcgtt
     8461 ttttcagcga gtttaccatt catgtcttga actgttatgc ccggtcaaaa ggtctcaact
     8521 tttttgccgg cgattcgctt attactctct gtgccacttt acgccttgtg cggtatcttc
     8581 aagaacgatg cccttggctt taagctcgtc acggatacgg tctgcctcag cccaatttct
     8641 gtccttacgg gcctgtgttc tctgcttaat aagagcttca atctcgtcat caaggctatc
     8701 ctttttacgg tcataaacaa gaccgagaac tcctgcaagc tcgtcgaata cctttatcgc
     8761 ttcctcgacg gagcccttgt ttgcgtttgc ggcaatgacg tttgaattga tgtcgcggac
     8821 aagctcaaac aaagcggcaa tagcgtcggc tgtattgaga tcgtcgctca ttgcatcgat
     8881 gaactgctga cggcgcttgt caaacattgc gagaagctca tcgctcactg ctccgtcctc
     8941 tgccttttca agcgcaaaat caagaccgtc gcggcaattg taaagtcgaa caagggaagc
     9001 cttgcactgt tcgattatat cggtgctgta attgatagga cttctgtaat gcgatgaaat
     9061 cataaggaaa cgaatcggct catatccgta tttttccgca acgtctctta cggtgaagaa
     9121 gttgccgagc gactttgaca ttttaacatt atcaacattg atatagccgt tgtgcatcca
     9181 gtagtgagca aacggaacgc cgttgcagca ctcgctctgt gcgatttcat tctcatggtg
     9241 agggaaaata agatcctgtc cgccgcagtg aatatcaatc gtcttgccga ggtagcgtct
     9301 taccattgcc gagcactcga tatgccagcc cggtctgccg tggccccacg gtgactccca
     9361 gtaaggttcg cccggctttg cgcttttcca aagagcaaag tccatcggct ccctcttgac
     9421 ctcgccgacc atgattcttg cacctgcttc aaggtcctca agcggctgat gcgaaagctt
     9481 gccgtactcc ttgaactttg aggggctgaa atatacgtcg ccgtcaactg cataggcata
     9541 gcccttgtcg atgagcgtcg aaacaatgtc gataatctcg tcgatattct ccgtagcctt
     9601 cggatgaacc gttgcttccc ggacattcat gcccttaacg tccttccaga attcctcgat
     9661 atatttctcg ctgacctcct tgtaggtcaa gccttcctcg tttgcacgct tgataatttt
     9721 atcgtcaata tccgtaaaat tctgaacaaa tcttacgtca tagccgatat actcaaggaa
     9781 gcggcgaaga acgtcaaaaa cgcaaagcgg tcttgcgttt ccgatatgga taaaattgta
     9841 aacggtaggg ccgcaggcgt aaatcttaac cttgccctcc tcaatcggtt taaactcttc
     9901 cttgcttctt gtgagagtgt tgaaaatctt catttatttg tcctccaatt tctttttaag
     9961 cttttcaatt tcgctctgca gtctgcaaag ctcctgggaa acgggatcgg gaatgtgaat
    10021 ctggtcaaga tcctgaaccc tttcgccgtt cctgcgaacg actcttgccg gaacgccgac
    10081 agccgttgaa ttcttcggta ttttatcaag gacaaccgca cctgcggcaa tgtttgtatt
    10141 atcgccgaca gtcacggggc cgagtatttt tgcacctgca ccaatcagca cattgttgcc
    10201 gatagtcggg tgtcgcttgc cgatatcctt acccgtgccg ccgagagtca cgccctgata
    10261 aaccgtaacg tcgtcgccga ttaccgttgt ctcgccgata accgtgcccg tgccgtggtc
    10321 tatgaaaaat cttctgccga tctgcgcacc gggatgtatt tcaattccgg tttttcgcac
    10381 acagtgctga gagatcattc tcgcaataaa aaacatatta tgattataaa accaatgtgc
    10441 tttcctgtga cttcgtatcg ctttaaatcc gggatacagc agcatgacct caatcgccga
    10501 ggttgccgcc ggatcacgct cacagaagca ctttatatcc tcttttattc tttcaaacaa
    10561 taaaaagtct cctttcaagt aaccgccaat atgcaaatac cggcagatac tcaacagctc
    10621 ggagccgaaa actaaagcct ccgccgcaca taatgcgaca gaggcggttt ttccgcggtt
    10681 ccactccgac ttataacttt cagtcctgta acggggacgt ccgcacagag atacttgcct
    10741 atgctttccc tctgcgtgct ggcgggtgca tttcatccga acgttcacaa gaggctttca
    10801 ccgttgcgcc tcactctctg ctgtgtccgt ttcgggttac ttctcccgat aaacgcattt
    10861 ttcatgtgtc attatatcac aaaattaaaa caaatcaata tctttttaga aattgccgcc
    10921 gttccagccc cccatttcaa cctcttcata cttaatatac acgtttgaag cagcaatatc
    10981 gagaacatcg ccgaagattt cgcatatttt tgcggtcaat ttgttggact gcgaataatc
    11041 tatcctgccg aaaaccttaa cctcaacata ggcaatcgga tttgaattat tgccctgaaa
    11101 atagaggtga catttgtcct caaagctgag cataagccag cgctcactct tgccggagag
    11161 aattgaaatc gcctcgccga gccttgattt gagcgtaagc tcctgctcgg gagaaattgt
    11221 aacatttgtc tttgtcgaaa tcattggcat aataaatcat cctttcatca ttgagtttat
    11281 tgtatctctt atcagttttt cgccctcatc ggcgttattg acattaagaa cagccttttc
    11341 catcgggcac ataccggttc ccgaacggtt aataatgtga tctaccttta tatcatatgt
    11401 caaaaccgta tttttatcat taagatatgc aacggcatat ttgcttgtta cttttgcata
    11461 gatttccgat acgccgtact tttcaagcag catagccgcc gccttgccga taacgcggtc
    11521 ggctgccgat gcgtccttta aaaaatcggg atcggtgtca atcttttgtt tgaagttttt
    11581 cgtaacatta aaatactgtt cctaatataa ctttcaattt attataggtt tctaacaaga
    11641 atttacttta atatcttaac gcaaaaacag ttatttttca accttattat gaatatcgtc
    11701 aatatttttc ttgaatttca atttcacata tgttatgata ggtttactca cgaatatgaa
    11761 ctgagggata aacatgattg cggaaaaaga aaagaccgaa aacaaagaga aggctcagaa
    11821 gcctgttaag aaaacagaaa aatacatttg gtttttagct tctgccgtta ttgcggcaat
    11881 cggagctgtt tgccactcca acgaaatcaa atttaacatt tgggtcagcg tcgtttactc
    11941 cgctttcctg ttgtttacca taataatggc agcccaacgc acgagcattg agcgcttcgg
    12001 ctatgccgat gaggaatccg aaacagcaga taagaccaaa tatgccctga catccattct
    12061 ttattacata tttatcataa tcgcagtcgc ctatgcgttc cttgctcttt gggtatccgg
    12121 agtgttctca atataatata aatctaaacc cgccaaaacg gcgggctttc ttttttcgga
    12181 acagactcaa taatatctca ttacagcaac aaccttaccg agaatttcac attcgtcaac
    12241 aatgatcggc tcgtaggtgt cattctccgg ctgcaagcga tagtgaccgt tctccttata
    12301 gaaggtttta caggtcgctt catcatcaat aagtgcaata acgatttctc cgtttcgtgc
    12361 aaacggagtt ttttctacaa ttacgagatc gccgtcaaga attccggcat ttatcatgct
    12421 ttcgcctctg atatgcagac cgaaaagagg cttatctctt gtcgccgtgc ctgtgtacgg
    12481 gaaatatccc tcaatctcct caactgcgag tataggtgca cctgccgtaa cggtgccgag
    12541 aatcggaacc tgagtgacat tctccgcctg tcccattatt ctgatggaac gcttcaataa
    12601 cgggtcacgc tgaatatagc cctcctcctc gagcgtgtca aggttggact gcacggagga
    12661 ggtggatctg agaccgaccg ccgcaccgat ttccctgacc gacggaggaa taccgtctgc
    12721 gctgcgctga acgagaaatt catatatttt acgctgagtt tcgttaatag acataggctc
    12781 aaccttcttt caaactttat acaactgtat tataacacat ttgttcggaa aaagcaaaca
    12841 tttgtttggc aaaaaatata gaaaattcct ctttattttt ttaaattttt gtgctacaat
    12901 tatataaatg aattgatttg aggttgaata tggttaatat ttggaaaaaa acagcaattt
    12961 ttatactttc tcttattctt tttaccgccc tttcggtaag ctctgtcatt gcggcagatg
    13021 cttccgacat tgcaagtgac ttttccgacg gtaaaaagaa tataatctgc atcgctcaca
    13081 gaggcgattg gcacagcttt cccgaaaatt cggcagaagc aatcaatgcc gccgcagagt
    13141 atgacgcagt gtccgtcgat gtacgcttga ccgcagacgg caagcccgtt cttatggcag
    13201 acgaaaaagt tgacagaatg tctgttgacg gcgagggcaa atccgtttcg ggtaaagttt
    13261 catcatttac acttgctcag ctcaaggagc tttatctgag ggaaagcaac ggcggaaccg
    13321 acaagaaaaa aacaacttgc cgtattcccg agctgaaaga gatttatgaa acagccgccg
    13381 gcagaacagc cgttatgctt aatgttcagg aaaatgattt caaagctctc ggcaagcttg
    13441 acgaaacagt tttcagaatt aacgcaaaac cccaaaaaat aattgagctg acgaaggatc
    13501 ttgacggagt taatgtaacg ggcaattatc agggcaacat catcttcctt gccacatcgg
    13561 cggtcaaaaa atattttgcc gctaatattt atacaataga aatgggcagc actaacggca
    13621 acggcgttct ttacgataat tttctcatga agcgctttgt cggcagcaaa agagctatgg
    13681 tctcaatggt aaacggcaga tgcggaaaga gagccgataa cga
//
LOCUS       DAWBSW010000032        13704 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_974_length_13704_cov_2.730530, whole genome shotgun sequence.
ACCESSION   DAWBSW010000032 DAWBSW010000000
VERSION     DAWBSW010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13704
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_974_length_13704_cov_2.730530"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8704..8713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3687 a         3128 c         3077 g         3802 t
ORIGIN      
        1 ccgcaatgcg gacatttaat gaaattgggg ttaatctctt tcttgcaatt tttacagcgc
       61 atgtttttcc tccgccgtgt atataatttt caggacacaa ctatcctgcc cgcacggcaa
      121 atatcttttt tgccgcacag cacagatagg ttatttttaa atcaatactt cggattgaca
      181 atgtcacgcc aatcaaggtc gcctctgtca agcgaatgaa taagagcctc agccgtggca
      241 atattcgttg ccgcaggaac gttgtgaacg tcgcaaagcc tgagcaggct tgaaacattg
      301 ggctcatgct ccttcggatc gaggggatcg ttaaagatga gaagcatatc tatttcgttg
      361 caggcaatga gagctccgat ctgctgatct ccaccctgtg agccacgcag gaatgtcgta
      421 atttcaagac ctgttgcgtc ggcaacaagc ttgcctgttg tgcctgttgc aaagagccta
      481 tgacggctga gaattccaca atatgcaatg cagaactgag tcataagttc tttttttgcg
      541 tcatgagcaa taatagcaat attcataaat tacctccgtt atattatttg taaagtgaaa
      601 aatcatacgg atcatatttg agtttaagat ctgaattctc acttgaatag tattctttaa
      661 tggaaatatc catctgatca agcatttgtt ccatttcctc atggttataa ggtttgatgc
      721 tctgcttatc ctctttgaaa aggcaatccc ctttcactcc gaaatgtaac tgaaatacgc
      781 aggcaaaaat gcaagcgatg cacgcagcga ttcttccgag gttcaaaagc tttccgcatc
      841 gctcagtcgg ctctgatttt aaaatcttgt ttgcaaagac agcatatgaa tacaccagat
      901 aaattgcaaa aaagattata gccggaaaac aggttcgaaa cagtaccgtt ttagtcatca
      961 ccatcataag ctgtgatccg taagcaagaa tcaaagcaac aatcggaaca aagtcatgct
     1021 tttccgtgta aatcaaaaca gcggaataaa gtgaaagaaa aacaaacaaa gcaaagtata
     1081 taatccataa aatcagaaaa atactgttcg ttttttcgcc gaaatcaatt tccctttgca
     1141 ggaacattcc gtaaaaagtc acgtagaacc tcaacaggga attcagaaca aacaggacgg
     1201 caaaaatcgg cattacaatt ccggcaacag tgcgtgtaaa tctattcttt ttcctaaatc
     1261 ggtaagacca gatgcagaat gacacggaaa gcaggataat cataatatag atgcttatgt
     1321 tatcaagcca gtttttcctt gccattgcca acagaacagt aaaaaaactt tgctcgttaa
     1381 gctcgccctg atttgtcatt cttgtgaagg ttccaggaga aaatacaatt gtcaggaagc
     1441 ccacaatcga aaaaacaatg caacctaaaa aatgcttgtt gaatttattt cggctgattt
     1501 tttcatttat aatcagcaaa acgaaccaac ctatgcacat cattccgatt tgttcaaccg
     1561 ttgcgccgca aataaatgcc agaggaaaaa gccactttga attttctttc ttcaaagaaa
     1621 tgaacattat aataatcaaa agcaaaatcg ggaacaaata gttcgtcgac cccgttagcc
     1681 agtaaaccga ctgattgtac atattcgggc gaactgtcat cagaacaaat gaggctgcac
     1741 aaactccgag cctgaacaat tttttgtcat caaaaaacag ccttggaata aaccaacaaa
     1801 gtgctgtcat cgctgccgca gaaagtatgc gccaaactgt aacagtaacg ctgttcctca
     1861 aaaagatgag aacgaaaata tgaacaagag ttctgccgtt gcatttcaat atgtgccatt
     1921 ttaaaaattg caaaacaacg ggaaaagatg aaacggcagg tttcatgtaa aaatagtcgt
     1981 caccaaagag atctgcactg agtgtcacac acaagattgc cgcaaatgtc agcagaagaa
     2041 taatgtaatg atagttattt ttaaaaaagc aagcgcattt tttcatctta acagctcatt
     2101 tcaacaataa taagtcaatc tttaatgcaa ttattcttgc gactgtttct ctgcggagtt
     2161 atcggcctga gttgctccgc cctctgcgtt ttcctcagaa ggtgtctctt cctcttcctt
     2221 ttcaaccgga gtgagagtta caatcttttc gccctcggaa actctcatta cacgaacgcc
     2281 ctttgacgga cgggcaaact tgccgattcc gtctgcagga atacgaatga taattccgtc
     2341 ggaggagatg agaatgatgt cattatcatc actgacagat gtaactgcgg caacatcgcc
     2401 gttcttttct gtatggtaat tgataaggcc cttaccgcct ctggactgaa cacggtaatt
     2461 gtcaaagtcg ctgacacgtc caaagcccgt ttcgctgacg gtgaggatag atttaccttc
     2521 ttcaacagtt gcaacgccga taacctcatc gccttcggca agctcaattg ccttaactcc
     2581 tcgtgcggtt ctgcctatcg gtcgcgcgtc gttctcattg aaacgaatgg acataccttt
     2641 cttggtcgca acaatgatat cgtcgtttcc ctttgtcatc ttgacccatg cgagctcgtc
     2701 gccctcatca agagtgatgg cgataacacc tgtgcggcgg atattagcgt actcggtaac
     2761 tgccgaacgc ttgataatac cgttgcgcgt aaacatacaa agatatttgt agtcgtcacc
     2821 tctgccgcag ttcattgagg agattgtctc tccgccttca agcggaagca ggttgactat
     2881 attcataccc ttactggttc gtgaaccctc ggggatttca taacccttga gcctgaatgt
     2941 tctgcccttc gaggtgaaga atgcaatatg gtcatgggtc gaagcggtga acatcgtctt
     3001 aacgatatcg tcctcgcgtc ttgtaagacc cttaattcct ttaccgcctc tgcgctgagt
     3061 ctggtactcg gacatcggaa tacgcttaat atagccgaaa tcggtaagag taaatacgca
     3121 ggtttcctca ggaataagat cctcgatatc aacctcgccg gaaacgttca ttatctccgt
     3181 gcggcgatcg tcgccgtatt tatcggcaat ttccatagcc tctgccttaa caagtccgag
     3241 gagcttatgc tcgtcaccga gaagctcgag aaattcggca atctttgctt taagctcggc
     3301 aagctcgtct tcaatcttga ttctttcaag gcctgtcaac tgaccgagac gcatctgaac
     3361 tatcgcctgt gcctgaacat cgtcaaggcc gaagcgggtc atgagggctt ctttaccttc
     3421 gttgacgctc tttgaattac gaagaatatt gataacctcg tcgataaaat caagagcagt
     3481 gagaagtcct tcaaggatat gcgctctgtc ctgagccttt ttaaggtcgt actgagttct
     3541 tctcgtaaca acttccttct ggaaatcaat ataattctga agaatctcct tgagagtaag
     3601 aattttaggc tcaccgttga caattgcaag aagaattacg ccgaaggttg tttgaagctg
     3661 agtgtaagag taaagctgat tgagaacaac ctgcggattt gcatcacgct tgaggtcaat
     3721 cgaaatgtgc atacctgcct ttgaggaata gtcattgata tctgagatac cctcaatttt
     3781 cttatccttg acaagatctg ctatgctctc aataagtctc gctttattaa cgttataagg
     3841 aatttcggta accttgatct caaagcgacc gttctttttc tcaacgattt ccgcacgggc
     3901 acggacaact atctttccgc ggcctgttgc ataagcggct ctcattcccg agcgacccat
     3961 aataatgccg taggtcggga aatcgggtcc cttgatgtgc tccataagct cgtcgagcga
     4021 agcatcggga ttatcaatga ggcagcacat tccgtcaatg acctcacgga gggtgtgcgg
     4081 cggaatattc gtagccatac cgacagcgat acccattgaa ccgtttacaa gaatattcgg
     4141 gaaacgtgag ggaaggactg tcggctcctt gagtctgtca tcgtagtttg ttgtaaaatc
     4201 tattgtgttc ttgtcgatat cggtcagcat tttgagagcc attttgctca ttcttgactc
     4261 ggtatatcgg taagcagccg gcgggtcgcc gtcaatcgaa ccgaagttac cgtgaccgtc
     4321 aacaagagtg tatctcattg aaaagctctg cgcaagacga accattgcgt cataaacgga
     4381 agcgtcaccg tgaggatgat agcgaccgag aaccgaaccg actgtgtcgg cgcacttacg
     4441 gtaagccttg tcgggagtca ggttgttttc atacattgta taaagtatac ggcggtgaac
     4501 cggtttcaag ccgtcacgca cgtcgggcaa tgcacgggaa acaataaccg acattgaata
     4561 gtcaaggaag gcttttctga cttccttatt gatatctaca ttatatatgg tggtattctc
     4621 cagattttct ttgaagtttt catccggatg ataaccgtcc tgcttagcca tgtctgtttc
     4681 cctcctttat acatcaagat tctgaacgta cttggcgttc ttttctatga aatcacggcg
     4741 aggctcaacc ttgtcaccca taagaatgga gaaggtttcg tcagcctcca ttgcatcctc
     4801 aagagtaacg cggagcatga ttcttgtttt cggatccatg gtggtctccc aaagctgaat
     4861 gggatccatc tcaccgagac ctttgtatct ctgaatgtcg cagccgccgc ccatttcttc
     4921 cgtgagtctg tcacgttcct catctgagaa ggcataaacg tgttttttgc ccttgctgac
     4981 cttgaaaaga ggtggctggg cgagataaac gtgacccgtg tcaagcagag gtctcatata
     5041 tctgaagaag aatgtaagca gaagcgttct gatatgagca ccgtcgacat cggcatccgc
     5101 catgataacg accttgtcat atctaagctt gtttatatcg aaatcttcgc ctatgcccgt
     5161 gccgagagcg gtaacgatcg ggataagctt ttcgtttgtg ataaccttgt caaggcgtga
     5221 cttttcaacg ttgagcatct ttccccaaag aggaagaata gcctggaact gtctgtcacg
     5281 accctctttg gcggagccgc ctgccgaatc accctcgacg atgtaaagct cggtgctgtc
     5341 cgcactcttt tcggtgcagt cggcgagctt gccggggagc gaattgcttt cgagcgcgcc
     5401 tttacgtcgt gcaaggtcac gtgcctttct tgcggcttct cttgctcttg atgcgtcgag
     5461 agccttggag aatatcgctt ttgcaacgga cggattttcc tcaaaatatg tcatcagctt
     5521 atcataaacg agtgatgaaa caatacctgt catttccgta ttgccaagct tgcccttggt
     5581 ctggccctca aactgagcct cggtcagctt aacgctgatg actgcggaaa ggccctcacg
     5641 gacatcctcg cccgagagct ttttgtcact gtccttgagt atgccgtatt ttcttccgta
     5701 atcgttgaaa acacgggtca gagcggtttt gaatcctgtt tcgtgagtac cgccgtcgat
     5761 ggtgtgaaca ttatttgcaa acgaaagaat gatttcgttg tagctgtcat tatatgccat
     5821 cgcaacctcg gcgtatttgt cgccgacggt cgttgaaatg tgaatcgggg gtgtgtgaag
     5881 cggggtaagc gcacggcttg tgttgatgta ttcaacaaag cttgacagac cgccttcata
     5941 gcagtaaacc ttttcaacaa cattttcggg atctcttgaa tccttaagct ttattgaaag
     6001 tcctgcgttg aggaatgccg actcgcgaag tcgtctttcg agaacgtcaa aatcgtactc
     6061 ggtcgtttcg gtgaaaattt cgggatccgg cttgaagcgg ataagagtac ccgtctcgtt
     6121 tgtatcgccg ataatcttca tatcgcaaac ggcgttgccg cgtgaaaact tctgatagtg
     6181 aacatgtccg tcacgggaaa cctcggcctc catccactcg gaaagagcgt taacaaccga
     6241 tgaaccgacg ccatgcagac cgccggaaac cttgtagccg ctgccgccga acttaccgcc
     6301 tgcgtgcaga acggtgagaa caaccgtcaa agtaggtata cccattttct cgtgcgttgc
     6361 aaccggtata ccgcggccgt tatcacggac tgaaattata ttaccgggaa ggatttcaac
     6421 ctcaatgtgg gtacagaagc ctgcgagagc ctcgtcgatc gagttatcga cgatttcata
     6481 aacaaggtga tgcaagcctc tcggacctgt cgatccgatg tacatacccg gtcttttgcg
     6541 aacggcttcg agaccttcga gaacctgtat ttgatcggcg ccgtattcct cggtgacgac
     6601 cgtatccata gcatcgtcaa gcagttcctc accgctcacc tctgccacgg caattatagg
     6661 ttcattcttt tcttcgtttg ccacggttaa ttacctccgt tcagtctgtt cataaatgat
     6721 tttttctgct tcttgggctt ataaagtggc ggatgctcca gctttgccct tacggatgat
     6781 tttctgagaa gcttgtttgt ttcaataacg gaaagtgtca tgtcgcccat atagtcaacg
     6841 cagtttgcaa gcaactgctc atcgtcatag agcgagccga gaatcgttac gacaataacg
     6901 ctctgagtct gatttgtgcc gtcattgtat atctcattta acatattaaa gagttttctc
     6961 atctgggtcg gatcgttgcg ctgaacggtc tcacgaacct ttgcgttacc gtaattgagg
     7021 aagaaatcgt cggcaagaaa ttcgccgtat ttcgtaacgt tggctctgta gacctccttg
     7081 agctcgggat agataacaac gaatctgctg ccgagagtgt tgacgtcata gtaagccgcg
     7141 ccgctctttg cctccgcctt ggaaacgggc tgagggagct tcttggagcc cttgttcttg
     7201 ccgccgtaaa cttcaattat accgtcgcag aaatcattga ccagatactt tatatctttg
     7261 ctgtcggctt tatcgggatt gaagagtgtc attgtgacct ttttatagtc atcgtcaaca
     7321 gcctccgctg cctgcgactg agcgcagagc aaagaaattt ttccctcaag gaattcaatc
     7381 tttaccgagc ctttcttacc tgtgtaggtg agtatcgagc tgccgttctt cacggcaacg
     7441 ggaatggcac ctttttcggt tccttcggga attaccgcgc tgaagccgtg atcgctcatt
     7501 gtgctttcta tacccttcat tatcaaaccg agagcttcgt taagttccat gtctttttct
     7561 ccttaaaata agcatagtta tactactgta cataacaaat aagattatat catataaata
     7621 ggtttttgtc actatttttt tgcaaatttg tatggagaaa tatcatcaaa aagctttttt
     7681 tcttgacttg atttgactat tatgttaaca taaactattg tgtataggta tagtgaccgt
     7741 tgatttaaac ccggtataat tttcagcggc gtttttttaa taaaatgcga aaaaatacct
     7801 gttaatatat cattatttta ggatgtgact ccaatgaaaa acgcacttat tcttttcggc
     7861 ggcgtatcga gcgaacatga tgtttcggtc gtttccgcac gctccgtaat cgagaacact
     7921 ccgagggata aatataatat tatccttatc gggataacaa aggacggccg ctggttcaag
     7981 tatgacggcg atgttgcaaa gctccccgat gacaagtggc ttgaggacaa aaaatcactc
     8041 acgaaagcag ttatttcgcc tgaccgtgag gatcacggaa tcattgtttt taatgatgac
     8101 ggaatcgaaa aaatacatat tgacgttgtt ttccccgtgc tccacggcaa gaacggcgag
     8161 gacggaacga ttcagggctt cctccaggtc gcagggattc cttttgtcgg ctgtgatatg
     8221 ctttcctcag cttgctgcat ggataaggca atgacaaata cccttgccga cgccgcaggc
     8281 atcccgcagg cgaagtggct cggcttcaca aaatacgaat acggcaaggc tcccgaagaa
     8341 tacaataaaa aagctgagga ttatctcgga tttccgattt ttgtcaagcc cgcaaacgca
     8401 ggctcatcgg tcggcgtaac caaggctgtt gatgcagctt ctctcaaaaa ggctatcggc
     8461 aaggctttca atgaggacag caaaatcgtt cttgaagaag gcgttgacgg aatggaggtt
     8521 gagtgcgccg ttctcggaaa tgaagaaccg atagcttcaa tctgcggtga gatagttccc
     8581 tgcaacgatt tttacgatta tgaggcaaag tacgtcaacc cgtcgagcga gcttcacatt
     8641 ccggcaagac tgccgaagga gaagattgac gaggtcagaa cggcggcttg caacgcatat
     8701 aagnnnnnnn nnnggctgca gcggacttgc aagagtcgat ttctttgtaa gacacagcga
     8761 cggcaaggtt atgctcaacg agccgaacac gatcccgggc tttacttcga tctcaatgta
     8821 tcctaagatg ttcgccgcat ccggaatccc gtacagcgag ctgatcgaca gactactcac
     8881 ccttgcaatg gagaagtggg acagatgaat tgcctgatta cgattcacga tatgcatctg
     8941 actgacggca gacccgagcg ttccgagatg ataacaaatg ccgatatcga gggcagtgca
     9001 gggcattaca ccgtgaaata caacgaaccg tcggacgaga tgaagggctg caaaacctgc
     9061 ataagcgtga cgggaggctg ttgtgttgaa ataacccgtg aaggcgcata caatacggtc
     9121 atgaaaattg aaaagggcag acggaatatt tgctgctacg caacccccgt gggcaacttt
     9181 atgatgggaa tttcaggttc aatggtgttc tcggaattta aagcaggcag gcttataaag
     9241 cttgaattct cttatgagct tgatgccgaa ggaacacttt tgagcaaaaa caggatccgc
     9301 ataacggcgg agtgaaaata aattataaga aacagcaaag cttctcagcc tccccttatt
     9361 gtaaggggag ggggaccgct tgcggtggaa gggataaatt gtctttatcc cctccgtcgc
     9421 taatgcgcca cctccccttg attcaaccaa ggggaggctg agtaaaaagt gcattgccat
     9481 ttaataagag aaaacgtaaa tttcctcaaa ccgaaaggaa tgatgtaaat gtcatatata
     9541 gtaaacgagg ttcagaacga acttaaaaaa ataatagcct gcgcttcgga aaaggctgcc
     9601 gaaaagggca cgcttgcagc cgttccgtcg ggcacattta atgttgagat ccctgccgac
     9661 agagcgaacg gcgatttctc gaccaatgcc gcaatgacat gggcaagaga gcttaaaaat
     9721 gctccgagaa aaattgccga tgcgattatc gccgaagtaa gccttgaagg cacatatttc
     9781 gagcgtgtcg aagtcgcagg tccgggattc atcaatttct atctcggcga ccgctactat
     9841 gccgatattc tcaaggatat ccgtgcaaag ggcaaggact acggccgttc ggactacggc
     9901 atgggccaaa gaataaatgt tgaatttgtt tccgccaacc cgacaggccc catgcacatg
     9961 ggtaatgccc gcggcggcgc actcggcgat tgtcttgccg cagttcttga ccgtgcagga
    10021 tatgaagtca gccgtgaatt ttacatcaac gacgcaggca accagattga caaatttgct
    10081 ctttcactcg acgtcagata tcagcagatt tataagggcg aggacgctgt tgagcttccg
    10141 gaggacagct accacggtga ggatataaag gttcgcgcgc aggaatttgc cgacgtttac
    10201 ggcgacaaat atataaatgc cgatgagact gagcgaagaa aggctcttgt tgattttgca
    10261 ttgccgaaga acattcaggc aatgaaggac aaccttacaa aatacagaat tgaatatgac
    10321 acatggttcc acgagagcgt ccttcacaac gacggcgagc ttgccgacac aatcgagctt
    10381 atgaaagaaa agggcttgac ttatgaaaag gacggagcac tttggtacaa gaatattgat
    10441 gttcagaccg agcgtctgaa aaagcagggt ctttctcagg agagcattga caaacttgag
    10501 cttaaagacg atgttcttat ccgtgcaaac ggaaatccga catatttcgc cgcagatatt
    10561 gcttatcatc gcaataagct ctcggtcaga aatttcgaca cggcaattga tgtttggggc
    10621 gcagatcatc acggtcacgt tgcaagaatg cagggcgcac ttgacgctat aggcgtcggc
    10681 ggcgacagac tgaacgttat cctcatgcag ctcgtccgcc ttacccgtga cggcgaggtt
    10741 gtcagaatgt caaagagaac aggcaaagca atcacacttg tagatttgct cgaggatatt
    10801 cccatcgacg cagttagatt tctctttaat atgagagaac cgggttcaca gatggaattt
    10861 gaccttgatc tcgcggttga gcagagctcg cagaaccctg tttattactg ccagtatgct
    10921 cacgcaagaa tttgttcaat tatcaaaaag ctcaaggccg aaggcattga gccgcgtgac
    10981 tgcaccgagg atgagcttga agcactcaag gctccggagg agcgtgagct tatcagacat
    11041 cttgccggac tcacaaacga aatcgttaat tcggcaaagc tttatgaccc ggcgaaaatt
    11101 acacgctatg ttatcgacct tgcaactcta ttccataaat tctataacgc gtgccgagtt
    11161 cagtgcgacg atgaagtgct tatgcaggct cgcctgtatc tctgcgtctg tgttaaggac
    11221 acaatcaaaa acattcttga aatgctccgt ataaccgttc cggagagtat gtgatttggt
    11281 taattacgga ttgttgcaac ctcaacatct tcggtaaagt atttgaaagc taaaatctaa
    11341 tgtctaaaag tttattatcg gaaaggactg cggaaatatg agaataaaaa acaataaaat
    11401 aatttcggcg gccggcaagg atttccttgc aaagcttttt tcgtcatggt ttttttcagg
    11461 agcggttctg ttcttcttcg gctcggggga tacgataagt gctcagcttt ttcagtcggt
    11521 aaacattttt gttgcaatca ttttgttcct tgtttttttt gccgcacaaa ccggcatacg
    11581 gtattttctt gacagcgacc gaaccgctcc gatatgcgtg ctcgcctcgg caagccttct
    11641 ggcgattgaa gcgtcgatga aggtaacaag cagctttgcc cttctcggct ttgccgctct
    11701 gatttttctc gccgcaagat atgcgtatct caaaaagctt gaaattgacg ttgcgcctaa
    11761 gacaagcttg gcaacggcta taggtgtagc cgtcatttcc gcggtttttg tatcggcaat
    11821 cgtcgttctc cgtctgctta tttatacctc tcctaacttt gatttcggaa ttttctgcaa
    11881 tatttattac aatcttaagg agagctttca gccgctttca acatgcgaga gagacaagct
    11941 tctttcgcat tttgcggtgc atttttcccc tattctgtat attcttttgc cgatttatta
    12001 tgtttttccc tcaaatataa ccctgcaaat tgcacaggtc gtcttgttga tgagcggcat
    12061 tattccgctt tggcttctta tgagaaaaaa caatctcaac tcaacggtaa agcttttttt
    12121 ggcaatagcc tactgcgctt atcctgccat cagctacgga tgtatttatg atttccatga
    12181 aaactgtttt attcttccgc tgcttttgtg gatgttcctt ttctatgagc tgaacaaaaa
    12241 aatcccgatg tttattttcg cttttcttgt gctcacggtt aaggaagaag cctttgcata
    12301 tgtattcatc tttgcctttt atatccttgc cgcaagaaag gactacaaaa aagctattgc
    12361 tttaatggct ctttcgcttg tctatttcgg ctttgccgtg ttctacattt cacatttcgg
    12421 cgaaggaata atgtctgaca gatttgccaa tctgaagatc cccggagaag gacttttagg
    12481 ggttataaag accgtattca aaaccccgat gcttgtgttt aacgagcttt tcagaagcac
    12541 aaacaacgag agcataaaga taatttattt cttacagctt ttcctgccgc tggcaatgct
    12601 tccttttgtg acaaaaaaat tctcaaggct cattcttgtt tgtccgttat tgataaactt
    12661 cctttcggac tactattatc agtgcgacct cggcaaacag tattcattcg gaatgacagc
    12721 gtttcttttc tatgtttcgg taattaacct gtctgagatc gagaggaaca agggcgtgta
    12781 tatgacgttc tccgccgccg taatgagtgc ggttatgatg ctttcgctga tgtacccgag
    12841 agttgtaaat tactcgctga gctacagcgt aaataaacag gcttacgaga gaataactca
    12901 ggtgcttgat gaaataccgg atgatgcaag cgtaacggct tctacatttt ttgtcccgcg
    12961 cctgtcgcat cgcagcgtaa tttacgaaca gtactatcac aaatcggttg atacggatta
    13021 tcttgttctt gatctcagag gaacagcctc cgtcaagata gccgaaatcg aacagccgta
    13081 tattgacgca ggctataaaa tgattgtaaa cgatgaaggc ctcatcaggg tttacgtcag
    13141 agattaaata caagacaccc atgtaagcaa ggctataaaa gttagatgca gaatgcattt
    13201 tcttttcaat gatgctgcac ttgtgcgttg cgagagggaa aagcactaaa aaaagcaaaa
    13261 taatttgcac cacacataaa acaaggctat aatccatgct tggtcatttc atttgattga
    13321 ccttctatga attatagcct tttctattta ttgttttatc tttggtctgc gctaaccggc
    13381 agcccatttt ttatttcctg agcggtttcg tatgccagat atcacgcgca taatcgttga
    13441 ttgctctgtc gcaggcaaaa cgtcctgctc ctgcaatatt catcagcgac attctgttcc
    13501 atgtctttct gtcggcaaaa agcttttcag ccttagcctg agccgcttta tagtctgcaa
    13561 agtcggcaag aaccatgtaa ggatcgttgt gtgtgatagt gccgcctatc tcggggaata
    13621 ccttgccgtt gattccgccg tttataaaat caacaacctt gcgaatatca acattgttct
    13681 gataatagtt catcggattg tagc
//
LOCUS       DAWBSW010000033        13455 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_993_length_13455_cov_2.297836, whole genome shotgun sequence.
ACCESSION   DAWBSW010000033 DAWBSW010000000
VERSION     DAWBSW010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13455
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_13455_cov_2.297836"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3344..3353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3849..3858
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5008..5017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5813..5822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10701..10710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12011..12020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3671 a         2785 c         3105 g         3834 t
ORIGIN      
        1 gattaaattg ctctgccgac gcttcaacct cctgaagctt gtcggaaatc tcggttcttt
       61 ttgcgaaact ttccgaaagc ttttccttga gagcgtcaat atagtcaaac tcgtttgaat
      121 cgactataat ttcatcgccc ttaaaggtgt ccttgagcgt gacgcctgcg attgaagcaa
      181 tttcttctat cttctgaatg gaagatgaat atgggttttc ctcgttcagc ggtacaaatc
      241 cgagcgagtc ggaaatgtaa tccattgcgt tttcgggatg aaaatcgccg ctgatacagc
      301 aggatgttat aaactcatcg aggtgatcaa taagaccgta ggtcctgacg agcttcattt
      361 tttcaattgc caaaacacag cctcctaact ttctgctccg atcaaaacgg aacgtgcttt
      421 atccgccgga atattgtatt ttatcgcctc aacgatgtgg ataatgtttc gcacctcgtt
      481 ttcggcaagg aaaaggtaac aaagcataac cgccgtcggg ttcgtataat aacgaaagcc
      541 cttgagctgc tgcccgataa gcatttctct ggtcgcattc tcaatgtatt tgaagtcaac
      601 ctttgaaaaa tcctttttat aataggtatt gggaatgata tttacaatgt ccgtcgcgtt
      661 tttcgcttcg gatatcgcag tcagatcctt ttcggtaaaa ttcgtgcagc cgggaacgct
      721 gaagtctcga atgaacatct tgttgggact gccgaaattc aaaagtctgt aagctcttgt
      781 gagcatgtac atatcaagcc tgtaattaac cgtttcgtaa atctcttttt tcttgcgccg
      841 atcctttgaa agcttgatgg atttgacaag aaaagagaat ctgtatttat taaggtcgtt
      901 ttcaatcagg ataaacattc cgtcatcgga ataattatcg cgatatcttt caagaatttt
      961 tttataatcc gtgtgttcaa gagccttcag aagatccgaa tagcttcgta cctttgccag
     1021 ctcataaaga tctatgtcgg ttttgctgtt gaagaatggc ggcagggaag agaggtattt
     1081 ctcgggcttg cctattatca acaactgtat aattgtcata agctgtctga tatcattctg
     1141 aacaacaaag taacggtaaa aattttcacc tgccgaaagc tgatattcgc aaagcttttc
     1201 gctcttcttt aaaaggctca tcttgagaat ttcttcgatc tgcgccgagt cgatattagt
     1261 cggcagggcg tcaaaatccg agcgataata tgttcttgtt ttaagatagt tcgcaagctc
     1321 gacaagtgat ttgcagttga gcatatcgtt gtagttctct tttgtcagcc ttttaccgta
     1381 catcgcatgc gcctttgcca taacggcatt gtcggcgtat gaaaccgcca ttacgttcac
     1441 ctcccgcagt gataaacgat cacaatatca caaaacaacc gggattaccg attgcctgtt
     1501 aattctttgt aacgctttca acaattttat ctacccattt gtcggcattc tctttataga
     1561 gattttccat accgttgagg atcttttgat ttctctcgcc gaccttttca agataggcgt
     1621 cgccctcagt gcgctgtttc tgactctttt tcaaagcgaa atcgagggct ttttggtttt
     1681 cttcctcggt aatgcgattt ttcttttcgc ccagttcggc aatttccttg tcacggtatg
     1741 cttcagcctc tttaacgcgc tcacacgctt gcttatccat atcaaggatc acgcgaagca
     1801 tctctttcat tctctcacct cattgcgctt gttaattctt tcacacatta tagcacttct
     1861 aaataaaaaa gcaatggtaa ttttgtacaa tttatggccg tttttttaga gaaaaaagac
     1921 aaatatttca ttgaaaaaaa ttgcgaagta ttgtagaata taaataacct gtgcaggagg
     1981 aaatcgttat gaagcttagc tatataattc ccgtgtataa tgtagagaag tatttaaaag
     2041 aatgtattga ctcaattctt gcgcagacat tcgatgatta tgaaattatt ctcgttgacg
     2101 acgcttcgcc ggataactgc ccttacatat gcgacgggta tgcggaaaga tatccggata
     2161 taataaaggt tatacacaaa aaaaatgcgg gtccagccgc cgcaaggaat gacggcttga
     2221 aaattgcatc gggcgattac atatggttct ttgacagtga tgatgtgctt aaggccgatt
     2281 gtgtaaacaa agtttatgaa atggctttaa aaaacaatgt cgatattttg caaatgtctt
     2341 ttatgtcttt tcatgacgga aaggacgact cctataaaac cgagtcggta tttgaatacg
     2401 gcaaggtgat cggacataag gaacttacaa agtatgttgc aagctccaat acggacagaa
     2461 gcgtgatttt tgcatggcga aatatatata aaaaagcctt tttagatgac aacaatgttt
     2521 tatttgaccc atcattgaga atgctggagg atccgccttt taatacgctt gcttttttga
     2581 aggcaaagag cttttcggca gctgatattc ccatatattc atacagaata aggcaggaca
     2641 gtctgcaaag aaaaaagtat gttgagaatt acgacatcgt gatggaatat caatggagct
     2701 tgaaattaaa atattacaga gatttttccg ataatgacgg gttgttttac aaagatatcg
     2761 ccgagtttac aattaaaacc aatctgcctg ttttgctttc aaatatatac agcaataaca
     2821 ttgacggcaa atatactttt cttaaaagaa tcggcaattc ggaaatgctc cgaaaatcct
     2881 ttgaagatta tgatatcaaa gaatttaagt caaaatcgct tgattggtgg gctacatttt
     2941 ttattaaaaa gaagctttat ccgcttgcac attttatttg caaaaatatt ctctataaat
     3001 agtatataat tataaatcgc acttcgtttt gaagctgcga tttatttttt aaaaccgctt
     3061 ttgtgacaaa aaaatcggaa aacgggagat ataatttaac tgaggtggtg aggatgaccg
     3121 tttatgccga tgtgcttgtc atggttaatt tgtttgtgaa ttatgcgttg ctgctttgct
     3181 cgtcgctgat tttaaaaaac aggatttcaa atcttcgttt gctcgcaggg gcggcgatag
     3241 gaagtgctta cggcttagtg atttttctgc cggagattcc gatttatttt gagctgccgg
     3301 tgagaatagc tgtgagtgcg gcaatcgttc tcacttcgtt cggnnnnnnn nnntcggctt
     3361 tcgcaatata cggcgttttt tgcgctgctt tttcacgttc ctcgccgtga cttttgcttt
     3421 cggcggaatc atgctcgtca tttgggtaac ggttgcaccc gtcggtatga tatacaacaa
     3481 cggaacggtt tattttgaca tcagcctatc cgtgctggcg gtatcaacgg ttgcctgctt
     3541 tgccatcgtc agcctgattt cacgccttat cgcgcgaaaa gctccgataa attccgttta
     3601 cacgctgaat gttacgaatg gcgaacgaat gatatcgggc gtggcactct gcgatacggg
     3661 aaattcgctc gtcgaaagct tcagcggtta ccccgtgata atcggcgaat atgatacact
     3721 gctcggaatt atgccgcaga gtgttattga ttattatgaa aacccgtcgg cgtgctctga
     3781 tgcgtcaaat cttcggctga tattgtgcaa aacggtttca aacgtgggcg ttttaccgtc
     3841 gttcagacnn nnnnnnnnga aaaatgttga ggtcgtttca attgacagga gaataaaaac
     3901 agatgaagtt tatatcgctg tgacgaaata ccggctcggc ggcggtgaat ttgattttat
     3961 ttttaatcca aaattgtttg acggagtgaa cgaccatgaa gataatagaa aaaataaaaa
     4021 agctcctgct tcggctgaag caggaaaaca ccgtgcatta tgtaaacggc cccgaaacgc
     4081 ttccgccgcc gctctcaaag caggaggagc aggcggtaat ggaggaaata ttgaagggga
     4141 acgacagcgt tcgggaaaag ctgatagtac ataatctgag acttgtggtg tacatagcta
     4201 aaaaatttga aagctcgggc gtcggtattg aggaccttgt ttcaatcgga acgataggac
     4261 ttatcaaggc ggtcaacacc ttttgccccg agaaaaatat caagcttgcg acctatgctt
     4321 cacgctgtat cgaaaacgag atattgatgc accttcgcaa aaccggctcg cagcgaaatg
     4381 aaatttccat cgacgagccg ctgaatgttg actgggacgg aaacgagctg ctgctttcgg
     4441 atattctcgg aagcgacccc gatttaatta accgcgatat cgaacttgag gacgaaaaaa
     4501 gtctcctgct cagaacgata aacgcaatgg gcgagcgcga aagaacgata atgtccatgc
     4561 gcttcgggct gaacggtcag cgagagcata cgcagaagga ggttgcggac attctcggaa
     4621 tttcgcaaag ctatatttca aggcttgaaa agaaaatcat tgcttcctta aaaaaggaaa
     4681 ttgagaaagc aacgtttgtt taatgcagct aatttaaagc ttcataaatt taaaatttcc
     4741 gtaaaagcag gtgtcaagaa actccgaggg caggtgagta ttctcgcaga aagccgagta
     4801 aagctccgca tcggagcata ttgcgttgat gcttctcggg atatttaaag aagcttggat
     4861 tcgtcggatc ctttcgatga tcctgtcaac cgaaaacgaa atatcttcgc ccgagcgaaa
     4921 aagaccgaca cgccgtgcca gctcgccgag ggcgtcgcaa cgattgtgct caagatagtc
     4981 cggcacacaa attgccgccg cagtttgnnn nnnnnnntct taatgccaag ctttgcaaag
     5041 aaagacgtga gttcatcgag catcaagctg ccgcagttcg tcgaatttct gtaagcgaga
     5101 acggcgcagt acattgcata ttgcaagcgg gcgattgatt tgtaatttcc tctgtaggct
     5161 ccgacagcat ggtttataat gcttgccgca ctcgacagcg caagtcggag acgactcgat
     5221 cagtgcatat aagcgaagcg ataacgtcat tatcgcagga gataacggca tctgccgcat
     5281 aggcgaagca tgacattgcg tcgtaagcga ttgtacccga ggttatctgc attgcaaggt
     5341 cggggtcaag aacgacctcg acaggggaaa ggcaatagat attgtaatcc gcctcgggga
     5401 aatcggtcat tgagcatgag gaaaaatacg gcaccgcggc aaaaccgaca ccgctttgat
     5461 atgaaatgaa catggcggcg tccatcagcc acgcgctgcc gaccgcaacg attctgtcgg
     5521 gagcattttc ggaaatatat ttgattgatt tttcgtaaag ctcgttaaaa tcctttgaaa
     5581 ccaaaagctt gatatccggc gttttttcgc cgagcatatc ctgaatataa ccgagggatt
     5641 ctgtttgttt aacggtctcg gcatcggaca gaatcaatat gttttcataa tatgattgcg
     5701 caaattttcg ggcggcaccg tcgccgaaaa acaccttttg cggcagagat atttcaggca
     5761 taaagctaca ctcctttttg acttattatt ttcaaagagg agtgttttat tcnnnnnnnn
     5821 nntaaccaat tataataacg aatatattag cgagattgaa aggaaaatcg aaatgaaaag
     5881 ctacaaaact cagtttgcga gaatatcgga cgctttcatc ggcgtaacgg cggttataat
     5941 ggcggtgacg ctcgtcgcct cgggcatcgc cgtatcaaaa ataaacacag aatatatggc
     6001 aacgggagtc aaaacagcaa aaatcgtagc cgaaagggag aatgaagaaa tatttttctc
     6061 gctcggcgaa aagaaattcc cctcgcccaa aagaataaat gataacatca aaactatcgg
     6121 atatcttctt cccgctccgc tcaacgccgt atattatata tatgaaaagg tgagtgagag
     6181 ggtgaagtga aacctgcttg cacacttccc gatgcggtaa acaatcttgg ctcctcctgc
     6241 gtggcagcaa aacatcggca aaggctttgt ggacgcccat tttataggcc acgttttcat
     6301 ctgcatttat taaatccgac aagggctggt gaaaagagga tataattaaa aattccctgc
     6361 gaattgcttc gcagggataa ttatttgtgc tgttttattt aagtccggag acggggatta
     6421 ttgtgccctt atcggttgct acggtgagtg taacggtgtc gccgtcgttg acaaggacga
     6481 tgttctcaaa gtctgccgcc ttgaccgaat agtagcttgc cgaggagtcg agcttaatat
     6541 aatatacgct ctcgccgccg ataacagcgg ttctgatatc cgtaacctta cccgtaacgg
     6601 tgatgtcctt ctgctcgctg ttcgtgtcgt ccttgatatt gtcagtgtca acgcttacct
     6661 tgatgttgtt cttggcaagc tctgcgacgt agttttcaat acagcttgtg agcgtgttgc
     6721 ccgttgcgcc gattgaatac tgaccgacgt tgatcattgc atagcccttg acgagcttgc
     6781 ttgaatcctt gagcgacatg aagtaggtcg gctcaccgtc aatgttgaga agaagcggga
     6841 atgaagcttc gtattcgtac tgctgaacaa gaccctctgc cgaggactgt gccgaggttt
     6901 ccttagcgcc cgaggtgctg taatacttgg cttccttggt acgctgattg ataaggacaa
     6961 agccggtgag cgattcatcg tttgtcagcg aggttacgcc cgtgtacata taaacgtcgt
     7021 cgttcattgc gaggtaggag tatccctcgg tggttgaata tgtgtccttc tgaccgagaa
     7081 cggagttcca gaagccgcgc tgatatcttc cgtagtagtc aaactgctca acaagcaggt
     7141 cggcggtata aacacggtcg atccattcaa ggtcgttgtt gttccttatc tcgtcgatcg
     7201 aatactccgt acattcgccc gttacgctgt cgacaatgat tgcgcccgtt acgtccgtgc
     7261 cgccgaaaag accgatcgtc ttatcaagct ttgcgaatat ccagtatggg tgacccgact
     7321 cgtcaatttc aaacgtcggc tcgtcgagaa gataggtcgg atacttgaaa cgaacgtgac
     7381 gtgttacata gtgtgcaaag tggtcggcgg tcgaatactt cataccgtcc ttgacctcaa
     7441 caagagtgga tttctgagtt cttgcgtcaa cgataacgta agccggaagt ccgttctttg
     7501 cattggtgag ccacttgatg atgttcgaat actggagaga cgcaactctg acgggagggt
     7561 cgtgatagtt gatttgataa tatttcggct gaaccgtgaa ctgcgaaaca tagccgagct
     7621 ttgaaagatc ggagagcgct cgctctgcca taaccgatgt tgtgctttca tcaattacgg
     7681 gaacggtcga gaaatctgct tcgtcaacct cttttgcaaa gtcgcccgtt ttaacgtcga
     7741 ttatctttga atagcttgaa gcacggaaga ataccgacga aacaagaaat ccgacaagaa
     7801 ctatcgccgc aagaacgccg atgaaaattt tcggcacaag cgaggatttc tttgcatagg
     7861 gcgcataatc gggagttttc agcgcaccgc tggtaacata gttgattgca cagtaaacaa
     7921 cggcgattgc gccgaagaag agatacattt tgatatcctt gaagttcatg accggaagca
     7981 tgaaataata aagaatgccg ccgaatatag ctgtcaaaat gatgttgata acgattttaa
     8041 gtcctttttt ttcgggagga atgacaatac cggtcttttt atcggggcct ttttccgctc
     8101 cgctgccggt aaaatctacc ttaatgggat ccattgaacc atctccttga atagtgtaaa
     8161 acacattata cattaaaata acataaaaaa caagatatat atttttctgt atcaatttgt
     8221 tttcaaaaag ttaacagttt accgccttga taaatataca attataggat ataatgaata
     8281 aataaaatat cgaaaagagg tgcaatatgg atagtcaata ttcgtattat caaaaccttt
     8341 ataatatgtt cggcggatat aaccctttgc ttgacaccaa ggtaaaacag ttgcgccggc
     8401 tcggctttat tatcggctgc ggaatggtct gcttcacgct gatgcaatat gccgttgccg
     8461 cattgcttca aatcttcgga ctttattcat tatatcagtc ggatgcaatt tttcaaacag
     8521 gcataagtgc aattgctccg attgtttatg ttcttttgcc gtttgtgctc gtttatgccc
     8581 tgtataaccg agaggaacga aacagcgttg atattttcga tttgccgaaa tcgaaggagc
     8641 ttttcacttt ttctgtgttt accggacttt tgatctgctc gatcggcgac agagcaacgg
     8701 cgttcatttc cgcattcata agtgcggcag gcgttgactt cgaaaagccg gaaggacttg
     8761 acgcaaacac gccgacaggt tatgttttgc agatagttgc ctatgccgtt attcctccgc
     8821 ttgttgagga atttgcaatg agaggcgttg taatgcagcc cttgagaaaa tacggcgaca
     8881 ggtttgcgat agttgtttcc gcagtccttt ttgccgctct ccacggtaat atggttcaaa
     8941 ttccgtttgc atttatcgca ggtcttgcac tcggatattt tgcaattatc acaaattcaa
     9001 tatggacatc tgttgcaatt cactgtctca acaatctttc ggcaacggta atttcgattt
     9061 attatacgaa tcattcggaa aatgatgtgt ttttcttcta cgcaatagaa ggtgctattc
     9121 ttattatcgg aatacttgca ttcattctct ttatgaagtt gaagccgcag aaattggcaa
     9181 aggctaacga cgagatcggc agctcgctta aatattcaac cgttttctgc actccgtcag
     9241 ttgttatttc gcttttgata tcgctattca cctcattttc cttcacgaat attacttctg
     9301 caggcggcgt ccttttctcc gttgcaatgg ttgcgctgat tgcattcctg cttgtcaaag
     9361 gctcaatgaa tgcaaagaag gatacaagaa taaccaaaag ctcaatgtac aatttttcta
     9421 ttgcaataac cgttatcgca tcgtttttta taatgtattt tgtgatgata cttccgctga
     9481 acaaataaga gggcagttta atgaatgacg aatattttat gaatcaggca attgcccttg
     9541 ccaaggaggc cgcggaggag ggtgaagtgc ccgtcggagc ggtcgttgtg cttgacggca
     9601 aaattgtcgg agcgggcaga aaccgccgcg agataagcaa aaatgcgctt gctcatgcag
     9661 agattgaggc tataaacgaa gcttgccgag ctttaggcgg ctggcgcctt tggcaatgcg
     9721 atatgtacgt tacgcttgaa ccgtgcccga tgtgtacggg tgcgattatc aacagcagga
     9781 taaagcggct cgtttacggc gcatctgatt ataaagcggg ttcatgcggc agcgttgttg
     9841 atcttttctc gcttccctat aatcacaagc ctgaggttgt ctcgggagtt atgcaggagg
     9901 aatgtgcggc tttgctcacg gatttcttta aaaaactcag aaaaaaataa tagttgtcaa
     9961 aatctctcct ttcatgtata atttgattat ggagggattt tttatgtcaa aaaataatat
    10021 gaaaatttca aaaggctatt caataatgtg gcttgttttt actgcgattt acgcggtctg
    10081 gatgtgcttt tttatgaaag cggatacata tcctgctgcc gagactggaa tcctcaagcc
    10141 gatttattac ccgatttggg ttgtgggctc gtgcgcaata attcttcttt acataatctt
    10201 gctaaacaga tatctttacg gtgagcttgg taagggcgat aaggcatttg cgctgataag
    10261 ccttgttttc ggttgcgtct ttatcacatg gtacggcttt ttcaaaaatc cgtttgagtt
    10321 cacggcaagc atgataggcc ttgaatatcc ttggcatttc aagatgtggg gaatttttgc
    10381 accgatttcg atttttgtca atacgattta tatgtatcgc aagttcggct acagcaacag
    10441 gggcggaata ataagcggtt cggtcggctg cgcggcgatg ttcgtcacaa tcaatgttcc
    10501 gtcggcaggc gaggagctta ttctcacgtc gctgagatgt atgtcgcatt ggacgggtgc
    10561 gcttgtattt gcattctgct gtgccgctcc gattgttatg ttccttttgc atatggcgaa
    10621 aacgggaaac aaaaaattta ttgcacttac tgttgcattc tgtgcggttc ttgtcacaat
    10681 gcttgttctg ctcgcaacgc nnnnnnnnnn gaggaaaaac agcctgcttt ggtttgattg
    10741 ttaaggagaa aaacgaaatg aaaagactga tttgcatttt actttgcatt acgtttttat
    10801 tttcgatgtc ggcttgcgat gttcggttga atacaacagg cgacgtaaaa aacgttaagg
    10861 tagaaaaatg ggaagcatca aagatttatt ccgatgagga gataaattcc gcaataaagg
    10921 cggttgagaa ctatttcaaa aaggaattcg gcggatgtac gcttacaaaa atcacctatg
    10981 ccggcgatac cgaatcgctt aatgaaacgg cgtacagaaa agagaacggc tatgattacg
    11041 ataaggtgat agttcttgtt tcttcgttcg aaacggattc gttcggcggc gacggttcgc
    11101 tgaaccctaa ttcgacgtat gacaattggg attggatcat gggcgaaaaa catggcggca
    11161 aatgggaaca tatcgaccat ggctatgcgt gagtttgagc atgggtgccc ttttagggga
    11221 gccgccgctt gcggtggtgg ggaagaaatt tgacagataa tacagaaaaa tcctcgttgc
    11281 aatgtgggcg tcgcatccta caattaggtt cggcaaaggc acgttccaaa taaaatttct
    11341 aaacaaaaaa gcagtggcca aaatgaccac tgctttttca tgctcttttt ttcagttcct
    11401 cgcaaagcct gccaagctgt tccattgtca gcgcttcggc tcggacggtg gtggagaggt
    11461 ctgcggcttc gatcgcttcg atgactgtcg gtttggaaat ccccatgcct gcacttatac
    11521 cgtttgacgc cgtctttctg cgttggctga acgccgcttt aaccattgag aagaaaaact
    11581 tttcatcgct gaccttaatc ggcggatttt tcagaatatc aagccgtata acagcgctgt
    11641 caacattcgg cgagggcatg aacgagcctc ttgaaacatc gaaaagcttc tgcgcttcgg
    11701 cataataatt gaccgcaacc gtgactgcgc ccgaatccct tgagccgaca tcggcgcaaa
    11761 gtctgacggc ggcttctttc tgaaccatta ccgtaacgga gtcaattctc agtctgcttt
    11821 caagtaagcg catgataacg ggggatgtaa tataatatgg caggttggcg cacacaacaa
    11881 cgggcatatt accgaattca tcttcaagaa ttctgtgaag gtcaaccttg agaatatcgc
    11941 cgttgattat tttaacgttg tcaaattcgc cgagcgtttc atcaagcacg ggcaaaagcc
    12001 ttgtgtcaag nnnnnnnnnn tgtcaagctc aacgcaaacg accttgcttg cacgttttgc
    12061 aagctctgcg gtcagcacgc cgattcctgc accgacctca agcacaccga cgccctttcc
    12121 tgcaccgcct tcttcggcga ttctcgggca aacgccgggg ttgatgataa aattctgacc
    12181 gagcgccttg gaaaatgtga agccgtgacg gcttaaaact gattttataa cgcttatatc
    12241 tgataaatta ttcataaata cctctttaaa tattgcattt tgaattgaaa acatatataa
    12301 tattacagta tatgataaat tttgtcaatg agggtgattt tatgaaaatt ttggtaacag
    12361 gcggagcggg ttatatcggc tctcatacat gcgttgaatt attaaacgca ggctatgagg
    12421 tggttattct cgataacttc tataacagct ctcccgaggt actcaaaaga attaaagagc
    12481 ttacaggaaa ggatttcacc ttctgcaagt gcgatatcag agatcgtgag ggtcttgaca
    12541 aggtttttgc cgagaacaag attgacgctg ttattcactt tgcgggactc aaggctgtcg
    12601 gcgaaagcgt aagaaagccg cttgaatatt atgaaaacaa cgtcggcgga acggtcacgc
    12661 tttgcgaggc tatgcgtgac gcaggctgca agaagatagt ttttagctca tcggctaccg
    12721 tttacggaat gaacaatccg tcgccgctta atgaaacaat gaaaatcggc ggcacgacca
    12781 atccgtacgg cacaacaaaa tatatgattg agcttatact tcaggatctc tatacctcgg
    12841 ataacgaatg gagtgttgca cttctccgtt acttcaatcc tatcggcgca cacgagagcg
    12901 gacgcatcgg tgagaacccg aacggaatcc cgaactacct tatgccgtat attactcagg
    12961 ttgcggtcgg aaagcttgat catcttaaca ttttcggtga tgattacgat actcccgacg
    13021 gaacgggcgt tcgagattat attcacgttg ttgaccttgc tctcggccat atcaaggcag
    13081 ttgagaagat tgcttcttca aagggtgttt gcatctataa cctcggcacg ggtgtcggct
    13141 acagtgtact tgatgttgta aaggcatttg aaaaggcaag cggaaaagaa atcaaatatg
    13201 aaatcgttcc acgccgtccg ggtgaccttg caacagtata ttccgacccg acaaaggcta
    13261 aggaggagct tggctgggtt gccgaaagag gtcttgaaaa gatgtgcgag gactcatgga
    13321 gatggcagaa gaataatccg gaaggatatt gataaatgga attttacgag aacagtcagg
    13381 agcccgaggt tgccgttctt gtcggtatcg acatgggact ttacaacgct caggtttcaa
    13441 tggtcgagct tgaag
//
LOCUS       DAWBSW010000034        13052 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1026_length_13052_cov_2.423175, whole genome shotgun sequence.
ACCESSION   DAWBSW010000034 DAWBSW010000000
VERSION     DAWBSW010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13052)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_13052_cov_2.423175"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2499..2508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3356..3365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9517..9526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3771 a         2899 c         2604 g         3748 t
ORIGIN      
        1 ttacaggcat aaggataccc cctttgttta ttgcttatgt taaattttca tcaatttatt
       61 atagtttcag gaattttact ttttctcttt ctttatccct tccacagtat gcgagacttg
      121 aaaagctttt ttatgtaatc atagaatttg catcaaaaaa acaatccctt acccaatagg
      181 ataaaggatt gtccggaaat attttgttga agactggtta aaaaaataat ttctttttgt
      241 aatgtgatgt catttaatga attttttaac atcggaagcg agataaagag aaccgcatac
      301 caaaactaca tcatcgtctt tcgccttttt caaggctttg taaaaagcct tttcaggatt
      361 cggctccggg cggacttttt tgcagtattg ctttacctct ttggcaagcg attttgcgtc
      421 aagcgttctc ggatttgaag cttctgtcgt ataaattctc tcacaaagag gagcaagctt
      481 tttgacggca tacctgtaat ccttgtcctt catcattccg aaaacagcga tgattttcct
      541 gtttgcgcag aattttacaa gcgtattgca aagagcatcc actccctcgg cattgtgtcc
      601 gccgtcaatt attgtaagcg gtgaatgtga aataatctcc gtccttgcag gaactttcac
      661 ctttgaaatt ccgtcaatca agctcttata tgtaacattc catccgttct gcttgagaac
      721 attgattgcc gcaacggcgg tcgcaaaatt ataaagctga tgcttgcccg tcagcggaat
      781 tttgaatgta cagccggcgt agattatcgt cgaactgtca atttcctcac gaacaaggcg
      841 gattttgctc aattcatgcg gaaaatattt gcaattcttc tcctttgcct tatccttaat
      901 aaaccgttca acggcaaaat caggctgagg ataaccgaca acattaccgc cctccttgaa
      961 aattccgcat ttgtgctcgg caattttcag tagtgtgtct ccgagaatat ttgtatggtc
     1021 gagagaaata gaggttataa cgctgacctc ggatttcttt atgatattcg ttgagtcaag
     1081 cagtccgccg agaccgacct caagcacgac aatatcacaa ttctgcctga gaaaataaag
     1141 aaacgctgtg attgtaataa tctcaaactc ggtcggttca atcccctgct ttgagagctt
     1201 ttcgacaaca ggcttgacct ctgaaataat ttccgccaaa tcctcccgtg aaatcatctc
     1261 accgttgatt tgaattctct cacgaaaatc gacaacaaac ggagaggtaa agagtccgac
     1321 cttatatccg ctttccgaaa gaacggaagc aatcatatga caggttgaac ctttaccgtt
     1381 cgttcccgca acatgaacgt atttcagctt atcctgcgga ttatcaaagg ctttgcatag
     1441 cgcctcaata cgttccatgc cgggtcggct gccgaattta tccctcgaat gtatgaaatt
     1501 caatgcttcg ttataattca ttgtatcact cttttattat aatcttgcct cgtctgcaga
     1561 ggcgtttaga aagcttctaa taactacgaa tgcgaaaaag aatagccggc taaatcatta
     1621 cctccccaaa ataaaaatga ggaggttgat tttaattttt acttttggaa tttctcgatg
     1681 ctctcgtcga tcatggcaat cttgtcacga agcttctgtg cggcttcacg ctgaccgtca
     1741 acaacagcct tcggagcttt tgcaacgaag ttttcgttat tgagcttctt gtcaataaat
     1801 tcaagatcct tgaggacctt ctccttctcc ttattgagtc ttgcaatttc cttttcaaaa
     1861 tcaacaagct caccgagagg gatataaatc ttagcgtcgg tcgtaacgat acaaacggcg
     1921 tcatcaatct caaagctgtc gcctatctcg gcttcggagc atgaagcgag tctctgcatg
     1981 aagataccgg ctttctcaaa ggtatcctta tgagccgtag ctatataata ctttgccttc
     2041 tttgacggcg gaacattcat ttcagctctg cggttacgga tagcacgaac tgcttccata
     2101 attctgtcca tttcctgctc ttcaacgctg aaatcgtatt catccttata aacaggccac
     2161 tgcgaaatca ttatggactc gccctcgtga ggcaaggtct gccatatttc ctccgtgatg
     2221 aacggcataa acgggtgaag gagcttgagc gtgttcgaca tgatgtaaac gagcatatct
     2281 cttgctgtct gagccgcttt ttcatcgccg gaattgagtc ttatctttga aatctctata
     2341 taccagtcac agaatacgtc ccaaatgaaa tcgtaaagct tctgaactgc gataccaagc
     2401 tcaaacttgt caaggttatc cgtaacttcc tttgtaagct tattgaaaag gctgaggatc
     2461 cacttgtcct caagtgcaag ttcgtcggga atatgcggnn nnnnnnnncc cgttgcaaga
     2521 gtaaatctga gtgcatctgc gccgtactcg tcaataacaa taagcggatc aattccgttg
     2581 ccgagagact ttgacatctt tctgccctgc gcatcacgga caataccgtg aatgaatacc
     2641 gtgtcaaacg gtctttcatg catattctca acagacgaga aaatcattct tgcaacccag
     2701 aagaagataa tatcatagcc cgtaacaagc gtgcttgtcg ggaaataatg agcaagctca
     2761 ggtgttttgt ccggccagcc gagagttgag aacggccaaa gtgcggacga gaaccatgta
     2821 tcaagcgtat ccggatcctg agtgagatga ttgcagccgc atttcgggca aaccgtcggt
     2881 gtttcctttg aaacaacaac ctcgccgcac tcgtcgcaat accatgccgg aatcctgtga
     2941 ccccaccaaa gctgacggga aatacaccag tccttgatgt tctccatcca gtgataatat
     3001 gtcttatcga aacgctcggg aacgaactta acttcaccgt tctttacgca gtcaatagcc
     3061 ggctttgcga gcggctccat cttaacgaac cactgctttg aaaccttggg ctcaattgaa
     3121 ctgtggcaac ggtagcaaac gccgacgtta tgcgaatagt cctcaatctt aacgatagca
     3181 ccctcttttt caaggtcctc aacgatcgcc ttacgagctt ccattctgtc cattccggca
     3241 tacttgcccc attcgggttt gatgtgtgca tcgtctgtaa atgtatcaat aacctcaagg
     3301 ttgtgtctga gaccaacctc aaagtcgttc gggtcatggg caggcgtgat cttaannnnn
     3361 nnnnncattt caacatagtc atcggcaatg accgggatct ctctgtgaac gatcggaaga
     3421 ataagcgttt tgccgacaat atccttgtat ctttcatcat tcgggttaac tgcaactgcg
     3481 gtatcgccga gcattgtctc gggtcttgtc gttgcaagct caagataacc gcttccgtcc
     3541 ttgaacggat agcgaagatg ccagaagtga cctgcctgat cctcatattc aacctcggcg
     3601 tccgaaattg aggtgaggca atgcgggcac cagttgacta tacgctcgcc gcgatagata
     3661 agtcccttat tgtaaagatt gacgaaaacc tctttaacag ccttgctgca gccctcgtcc
     3721 attgtgaaac gctctctgtc ccagtcgcag gaggatccca ttttcttgag ctgttcgata
     3781 attctgccgc cgaacttttc tttccactcc caggctcttt tgagaaagcc ctcacgtccg
     3841 atatcttcct ttgtgatacc ctctttacgc atagcttcaa cgattttagc ctcggttgcg
     3901 atagaagcat gatccgttcc gggaagccac aaagcgtcat agccctgcat tcttctgtaa
     3961 cggataagaa tatcctgaag tgtgttgtcc agcgcatgac ccatgtgaag ctggccggta
     4021 atgttcggcg gcggtataac tatagtatac ggcttcttat ccgggtcgca ctttgcgtga
     4081 aagaactttc cgtcgagcca gaatttataa attctgtcct caacgtcttt cggatcgtac
     4141 tgtttagcaa gatcttttgc cattgaaata tcctccttat gtcaatgcag ttaaattaca
     4201 gtataaataa aaactcgcct caaggaaaac cttgagacga gataaaaccc gcggtaccac
     4261 tcaatttgta ttcaaagaat accactcaaa accttaacgc agaataacgg gagggttcta
     4321 cggcaaatgc tttcttccct ccggctcggg agctacaatc cgaataacgg ccgacgtctc
     4381 tcacctgccg acgcctctct gaaaaccgaa caatccgaat actctccgtc tttgcctttc
     4441 gatatcgcta ttattctatc atcacgggag atttttgtca atagaaattc actttttttc
     4501 gttcgccgca cggccaagag ccgcttcgat atttcccgaa acggttatgt tgagcaagtg
     4561 aataagctcc tgactcgaca tccgtgccgc gccgttagtt tcgagccagc tctgtaccat
     4621 tccgccgaaa gctatcgcat agaaatttga aataaagttt ttatagtact cgtcaatatt
     4681 aagatctttc gactggaggt caacaacacg ttttatactt gtccttaacc cctcgtaaac
     4741 gaagtttgaa aaataaacca tggctcttga gtttataacg ctgagaattt catttctatg
     4801 ctcctcaata taattgaaaa gagccttgat tacctcttcc cagtcatctc ttgtgccaac
     4861 ctcaccctga agaaacgctt tgttgtttct ctcgataagc tcaaccgcgt catgctggta
     4921 catccactcg acaagagcat aaatatcctg aaaatgataa taaaatgtct gtctgtttac
     4981 cccgcaggca tcaactatct catggatttt gattttatca agaggttttt tccgcatcag
     5041 atttcttaac gattctgcaa gcatcagttt tgttttatca cccgaacgct gacttgccat
     5101 tttatcactt cctttatgaa tttgttaaaa ttataacaca ctattataca tatatcaaga
     5161 cattttacta tacagttgta tatacaatat tgggatattg taagattttt agcaaaaaaa
     5221 tatagcctgc aaaaagcagg ctaaagcttt ataaatactg tctgatttca gccgtgaaat
     5281 cgctttcgat tttaatcagt ctttttgtca gttcctttga cgcatcatcg gcggctttat
     5341 actgatttag atatcggcag agggatttta ctcccatatt gcagccgtcg gtcatcaaat
     5401 cggcaattgt ttcatcggat ttttcggcag ccattctaaa atttgtttta agctgtgaca
     5461 ttcccttaat aaaaggattg gggtttttgc cgtcatcgtg atacttgccg aggagctttt
     5521 ggatatcgcc gttgagctgc tgatggtcgt ccttcaaatc tatgagcttt gctttaaacc
     5581 gctcattttc aacatcggaa agcacctcgt ctatcgcagc aatccccatt ttaactccgg
     5641 cgtcgcactc tctgagaagc cgtattgtat cctgttcgat catttttctt cacctctacc
     5701 ctatttttac cgataattaa aataatatac aataataaaa gaacggataa agagcggaaa
     5761 ataaaaaaat cgacaaccct caaaagaaga ttgtcgatat ggtccgagtg gcgagactcg
     5821 aactcacaaa agcacattta atgagcttca atgaagtgca acaaacgttg atatagagcc
     5881 atttttgaag cggtgagctt caatgagctt tgatgaaagt aaacaccaaa cgacgagaat
     5941 aaacaccaaa taaacaccaa ataaacacca aaattcagca gtcgggcatt gacaagttcg
     6001 gctatattta atataatgct cttgtggtgc taccatttac ggtaggcggt tacactctgc
     6061 gccctcgtga gggggtgatc ttatgcagta tgttacatac gaaaacttat ttactttcgc
     6121 tcttgtaatt atcggacttg tgacggtgtt tgtcgcagtc cttgcatata aaaagaaata
     6181 accgcccaac gttccaaagc taaggcggtt aaatcttttt acaatcttat ttgagggcgt
     6241 aaccgtctat cggtagcacc actctttgta tcttcattat atgctatatg ccttgaattg
     6301 tcaaggctat ttttatgccg taaaggtaaa gaaatgttaa gaaataacgt tgtcacaagc
     6361 tcggcaaagt cttatatatc aatgcttcag ggcgtttttt aattataagg gcttacccct
     6421 aaggaatttt gtgaaattct cacgcttcat atgagacgct cagagccgat ataacggctt
     6481 ttatctttaa taaccgagct tgccgataaa acctataacc tcaagaattg acaagctcgg
     6541 cagataacaa aataacggta cacttgctac ggtgtaccgt ctttttttat gtctgttttg
     6601 tttctgtcgg tgttatagtc aaagcgtttg cgatcgtttc gctcgccctt gcttgcgctt
     6661 cttggaagct gtgagcatat atattcaagg ttgttgacgt ctggctatga cctaacgcag
     6721 ccgagaccgt gcgaacatca acgccattca taataagcaa tgaagcgttg aggtgtcgga
     6781 agctgtggac gtttacttgc cttaacccgt tccgcttgca gaattttgta agccaatgtc
     6841 gagcgctgtc aacgcccata ggtgcgccgt tctcggcaat gaatattttg tttgaattaa
     6901 cccacctatc gcccattaaa agccgctgct tgttctgctc cgctttatat tcacgcagca
     6961 cgtcaataac gttttcgggg agcttcaagc tgcgttgtga tccttttgtt ttcggtgtac
     7021 ccgtaaacgt gccgccctgc tctttgtcaa ataaagaagt acgcataaca gatataacgc
     7081 ccgtttcaaa atcaagatca gaccattcaa agccgagaag ctcaccacga cggaagccgc
     7141 cgaacatagc aagtgtaaaa aaggctttat attttatcgg tgcagtttca agcagggtta
     7201 agaaagtcct tgcttcatca atcgtataac attcccgttc tttcgggtct ttttttataa
     7261 cctttacatt ggtacacggg ttcttgacgt caatatcaca atcaatagca taattgaaca
     7321 cgtcggaaat gaagttcaaa tacagtttct gtgttttggt agaaagcccg ccgcccgtct
     7381 gctgatttat gccgtcctca gcaaggttat ttataaacgc ttgaatatgt gttttattga
     7441 ttttattcat tttcaagtgt ccgatcgcct tatatgttct ctcctggcat tgcttaaagc
     7501 gttttatagt aagcggcttt aattcgcccc ctctctcgat ctgcttcacc cactgccgag
     7561 cgaactcctc aaattttatg ttaccgtccg ttgtaaagcc gttttttata ctttcctcaa
     7621 ataacaacgc ttgccggtgc gcttcctttt cggcttttct ctttgtcata ccggcttcgg
     7681 gcttgtaggt cattgatcgc cgtatctgct ttccgttaag gtcatagcct gccgataccg
     7741 ttatacggta cgtttcccct ctcttttcaa tcgttgccat aattaccgcc taacgtctgt
     7801 gtaaaaagtc ttgcaagtgc aaaagctgga tcatctgcta atttcttttc taattcttcg
     7861 tttgcttctt caaaataact atccttttct aacaatgtat tagcaatttt ttgaaattta
     7921 agtgcattta tttcgcacaa aagttccttg ttgcaggctt cacgcccgaa tttttcattt
     7981 tcttcgggtg tttctgcatt taaatatttt tgtttgtacc aaacggaact tgaaaaattc
     8041 attttataca tatatgcaag ctcggataat ggcagggcat attcggaaat aaatttattt
     8101 gtaaacttta acatttcgat attaaataca tctcttccgc cgtcttcgag tacctctgtg
     8161 ttcttatgaa gcgttgaaat tgaagtatct gttaaccccg tataatcgca aataaaccgg
     8221 atatctttat cggttgtcgg tgtatccgtt tttcccacca agtaatcaag ggaaacgtta
     8281 aaataatctg cgtatctaat cgcaaaatct aaagtgatag agcgattgcc gttgtagtgc
     8341 tttgtaacca aagaagtgtc gcagtcaaga gctttagcga ctttttctct tgttgtttta
     8401 tcaataagtt ttctaaagtt tgaaatgtta atgccttttt tgtcacacat agtattaaat
     8461 caacccgttt cttttagttt tgtcacaatt ttattttttt gtgacttttt cgttgactta
     8521 gagattttgt cactttataa tgagattgtg acataacagt catcacgact attctaacaa
     8581 acaatcatca ttatgtcaac agaaaggatt gaaagaaatg acaaaaacag caaacctcga
     8641 attgagaaga acagcaaagg cgaacggtgt tgcgctatgg cagatagccg acaagctcgg
     8701 cataagcgaa cccacgatga cacgccgcct gcgcttcgag cttcccgaag aggaaaagga
     8761 taagattatc ggcattatta ccgagcttgc aaaggagtgt taaagaatga aaaacaaaaa
     8821 cataaaaaag aacgcaccga taagagatac accatttaca aaaaggcagt taagccgtgc
     8881 tgatgttatg gatatcatta tagacttgca acaaatacaa gaaagcattg ataaaactat
     8941 taaaaagtta tattcactcg acgatgaatg gactattatt cctgacacgt cgtttcttga
     9001 gggggaagaa caatgacgtt accgaaaatg agaacagcac ccgaagcgat caaggagatc
     9061 aaggagcttg accctcgaag ctgtctgaca cttaacgcta tacgcagtat gatgagaacg
     9121 ggtaagatcc ccgttacata ttcgggtaca aagcgtttaa taaaccttga tttgctcata
     9181 gaacaccttt acaaggacaa caccgagctt gcgagcgaac ccgaatacgg caaaatccgc
     9241 agattatcat aacaaagcaa aaaaagaaag cgcctgccga agcaagcacc cattgagata
     9301 ttgaaatata ctgcacaaat attttaatac atcgtggggg ttgtgtcaat ggcaacaaat
     9361 gaagaacttg cattacttat tcagcaagga cataaggaat atatttccga gctgtgggcg
     9421 aactgttata aacttctgta tatgcttgca gataaaatct attatgcgaa ccccgacagg
     9481 ctcgccgcct gcggctatac gcaagaggat ctatatnnnn nnnnnnatct atatcaaggg
     9541 tgtttctttg tgcttctgaa aatgatcgaa gcatacgacc ccgaaaagcc ttttaaattc
     9601 acaagttatg caaagctcca tttacaaaac tattttcggc gctccgtact gcattatgac
     9661 agcgaagcga agataataaa aagcgtttta aatatctcaa gctcgattga taagcctatc
     9721 ggaagcggtg aagatgaaga actgacggta ggaagcctta taaaagacga taatgccgag
     9781 cttgcatttg aaaacgtgga agaagatatt taccgtaccg agctgcacaa cgcccttgaa
     9841 cagggcatac agacgttatc agaaccgcag cgaatagtta ttcgggaacg tttctatagc
     9901 tcaagcacgt taaaaggtgt agcggaaacg ttgggagtga ccgaaagtca ggcacattcc
     9961 cacgaagcta acgcccttaa agccttgcgt acatataatg ccaaaacaaa gcagcttgaa
    10021 tgtttccgag ctgatattat cgacacggca gcatatggcg gcgtaggcgt tggagcgttc
    10081 aagaacagac aggcaagctc ggtagaatac acaacagaac tactcgaaga aaggaagcaa
    10141 caccacaatg acaaaaagta ttattgatat cgaaagagaa agacacgccg cccttgcggc
    10201 aagcaggacc ttaaataata accgtattac tcttaaccct gcccttgagg aagaagatta
    10261 cgaagagtgc tgcgttattg tcggcggtcg agaatggcac tcaaattgta tgtcacctgc
    10321 cgagcttgca gagttcgcag aattggcaaa cgcttgccat ttctttaatt aaaactatat
    10381 agatctcccg aaaaaattac acggcgaaaa gccgtgtttc gggaggaagt gggtattaag
    10441 ttaccgacga aaaagagaaa ggagcgacac gaaaaggcta tgttattacg agagaatata
    10501 cacaagtcgg gtgattattt tgaagtcgat atattcccga tcaaaaagat tgcttgcggt
    10561 cgctcctcaa agctccacct tacctctgaa acacaaagga agctaaatga acgcaacgtc
    10621 caaaggcggt tgacgtggat aattcaagag aatttcactc cggaagacgt tttacttact
    10681 cttacatatc ctcaaaatgt tcatataacg cccgaacagg gaagaaaaga ccttgaaaac
    10741 tatctgcgcc gtatcagata tgcccttaac aagcagaata ttaaattaaa atacatatca
    10801 aaaacagaga tcggcacgtt gaaaggcaga gtacatcatc acgttattat ttcaggtgac
    10861 ttaggacgtg attatttaga gcagcaatgg aaagacaaag taaacggatt agctacaaca
    10921 cgctatatag agttctacga aaacggagta agcggactgg cggagtacat aaccaaagat
    10981 aatagccttg cttatcgctc gtataattgt agtaagaacc ttaaaagacc tgaagccgag
    11041 aaacaagacg gcagaataac aattaagaag ctgcaagact tacgcaacga tatacacaac
    11101 gctgaagcgt tcgagagatt ataccccggt tatcgttttg ttgaagctga tccgttcttt
    11161 aacgagtatt tttttgacag tgtaagcaga gaggttgaac gggtagaact tccgtacata
    11221 aacatcaaaa tgtataagaa agacagcacc tacatacggc gagggtttag gtaagctctg
    11281 ccgagcttgc tatatctcgc tatataaata aatacaaccg tcgagcttgc cactcccccc
    11341 cattcttcac gccgtgagcg gctctgtttg accgaacgct accccttaaa attaccctgc
    11401 attgcgtgcg taacgggtgt ggtatgcgtt gaaagaggtc aaaagggctt aatttgcttt
    11461 aaagcagagt aaatacaagg ggtagccgta attctgtaag cctatacggg gataacggta
    11521 caagaggaat gttttatgcc aaaaactccc cacgggaaag ataaaagccg ccgcccgacc
    11581 cttgaaaagt gaaaagtgta ctataaatct ttgtttttgc aagctcggcg agcttctcag
    11641 cgccttgtat gtcaatgctt cgggagttca tataaattct ctgcttttgc tcaaacgtcg
    11701 ctaaaatcgg cgcagaacga atgatataaa gtgtgtgttt ttaggttatt tttgcggaat
    11761 aaatcaaaga aaaagttaaa aaagcattat agcaagctcg gcagaataaa aaagaaagga
    11821 aatcacaatg cagaataaaa ataaacttcc cgatcacgtc agagcggcag cattggcagt
    11881 tgttaaaagc tatgaaccgt ataaacgtga tatcgaaaca gaagaaaacg aatgtaaata
    11941 cagcttcgtt caagcggtcg aaagcgcatt agatgaaatc ctcaagggag aaaatcaaat
    12001 atattcttta aagctcagac aagctctgct tcaatcgtgc attaaggggc ggaaattctc
    12061 tttcaattat tcgggtattg actgtatgag ctgttctacc ttttaccaca gaaaaaatca
    12121 gtttttatac ctgatcgccg acaagctcgg tcaaatttga agaaatgcga agaaaaaacg
    12181 ggattttcaa gcaccgccga agccgccgag gcccttattt atcaaggctt tcagggatca
    12241 aaaaccgccc gaaatagtta acctcggttt aagtacccga aaagcccctt gaaacgccta
    12301 taaatcagca taaaaaaagc cgccgaagcg actttaaaat atatccgccg agcttgtcgg
    12361 ctatgtatca actatcattt ttgctacggt attcatcata aagcattaaa gccgtaaaaa
    12421 cacaagaaaa tgttcttgct tcatcttctg cgatatcgct gtcaaatgag ccgcttaaat
    12481 gcccaaataa tacgtgtgca agttcatgag cggctgtcat ttgttgtgtc catttagaac
    12541 gctcggtatc aacaaaaact acaggcttat tttcatagtg agttgcaaga cctaaatagc
    12601 cctcacgctt tgtttcttct ggcattacat aaagacctat tccgagatct ttatatattc
    12661 gaccgagaat acccatttta acactccttt acgactgtaa cgcctttgat ttgctcatac
    12721 attcgcttaa agcggtctgt atttgctcgt gtagttccct tgccttatcg tcaacggcat
    12781 aaattaaagt tatcataaga tccacacgtt gagcataagc cattcggcgg cattgatcct
    12841 cccgaccgcc tgcgtgatcg cattcttcgg ctgcaagctg caagaagttc ctttgcacat
    12901 caagtgcggt aagtgcttca tcaagaacgc acactacttt tggcattaaa tcatccattg
    12961 tcgcatatcc cctttgttat ttagtaagcg gtcgagcttg cggctcttgc cggtgtgctt
    13021 cgttgctttc ggcggttaag ctcctgacgg tg
//
LOCUS       DAWBSW010000035        12964 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1037_length_12964_cov_2.700287, whole genome shotgun sequence.
ACCESSION   DAWBSW010000035 DAWBSW010000000
VERSION     DAWBSW010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1037_length_12964_cov_2.700287"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    969..978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2058..2067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6194..6203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3491 a         3042 c         2705 g         3696 t
ORIGIN      
        1 ctacgatagc tttatcggca gctcttgttt tgataagctt ttcggtactg cttgtttaca
       61 gctcggtatc ggtcggtgaa aaagcatggt acggcggcat tacgtccaat aaaagaaact
      121 tttcaaaaag atttttcttt tggcttaaac caaaaaattc cttgcgtgct tttcgattca
      181 aagcattatt atttctttta aaatccatgt gggctgtcgt attctttctg ccctcggcaa
      241 ttctcgcttg gtccgtctac tacctttcgg gcacgggagg acttgagctg tatctgttta
      301 tttcgctttc cggcggaacg tttttgctga caacaagcgg agctgtattt tattttattg
      361 ccatgcaaaa atattttata gcggaatatc tcttttcttc aaatccaaag cttggcgctc
      421 tgacggcgat aaagcaaagt aaaaatctgc ttgacggtca tatttatgaa atcgttcgct
      481 tcaaattgag ctttttaccg tggtttttta gctgtatttt tatttttccc gttatttatt
      541 tcctgccata ttataaagaa agctgctgcg ttgttgcaaa aagaataact ttgtgataaa
      601 ataaaaaaga attttttcac ggagatgttt atatgaagga taacttaaaa tggacaaagt
      661 caaaagtttt tttgatatgc ataaactgta ttctttgcgt tgcaattgtt gtcagcgcga
      721 tgatgattgt aattgacaaa tcgagaatta aaaacggcgc tgttttcacc ggcggcgaaa
      781 aagaaacgac cgcttccgac tcttcggtct ctggaacggc cggcagcgca aggctgatga
      841 ttgccggcga caatgtcgtt tacaaaaccg tttacactca ggcaaatcaa aaagccggcg
      901 gcagcggata tgatttttca tccgcatatg acggaatcaa ggaaattatc gccaaatcgg
      961 atattgcann nnnnnnnngt caaaacaccg ttatggacga caaaaacgaa ccttcggcat
     1021 ctcctgaatt taacacgccc gaccagatgc ttgataagct tctcgatctc ggatttgatg
     1081 tctacaatca agctaacgat cacatcatgg atatgggtct ctcgggtgcg ataaacgaca
     1141 ttgccctttt caaggcaaag gaaaatcaag cacttttaac aggtctctat gaaaacaaag
     1201 aggaaatgat gaagccgcag gtcaaagagg tcaacggaat caaaatttcc tttgtcggca
     1261 taactcagtc actcggcgat tacgagcttt cggaggaaac ggatataggt atcctcaatc
     1321 tttccgacag ccgttcaaca tcggacgagc ttgagggcac aatgaaacag cttgtcgaaa
     1381 gcgcaaaaaa cgcttcggat attgtttgcg taacggttca ttgggacggc gaatcaacaa
     1441 gcgagctttc cgatactcag aaagatatta tttcaaagct tttgaaatac ggcgccgata
     1501 ttatcgtcgg cacgggtaaa aacgacctgc aaaaattcga atataggtca aacggcgacg
     1561 gtgaacaggc acttgttatt ccgtcactgg gcaaagtgat ttctcttgag gaaagcgtta
     1621 acagcttcct cggcggaatc gcagatatca ccgtttcaaa agaccccaag acgaacaaaa
     1681 cgcacgtctc ctcggcaaaa cttataccga ccgttacagt ctatgaggag gattacgcaa
     1741 acgtccgtgt tctcccgttt gcaaagtgca ctgaggatat tatcaaaaag catggatttg
     1801 ttcttgagga cgaaacattt acctacagct acattaaaaa ttattacaat caaaagttca
     1861 gcgatacgct tgaattgaat tattgataaa gaaaaaccgc tctcacaaag ggcggttttt
     1921 acaatgctta aataaattca atatatattt tttacagact ttcttggtgc aacgaggttt
     1981 aaaaaatgaa cattccgtag gggtcgatga ccacatcggc ccaaggtcag gtgaaagagt
     2041 gtcacccgtc tcccctgnnn nnnnnnnaac tgccacaccg aaatgaacga tgtgacagtt
     2101 tttatttatc tgttaataag cggtttgacc tgtttgccca aaagcacggc gattatcatc
     2161 ttcgcacagt caagcgcaat gtagggaata actcccgcaa gaagagcctg cttgaggttc
     2221 atgcctgcct gataagcgag ccaaagtgaa ccgaaaaggt aacaaacagc tgttccgagc
     2281 gccatgccca aaagtgaaaa gaaaaacttt cctttgaact tatcgacgaa tattccgccg
     2341 ataacggcaa tgaagataaa tccgattata tatccgcccg tgggtccgac aagcttggca
     2401 gctccgcccg tgaagcccga gaaaacaggt acgccggcaa gaccgagcag aatataaata
     2461 ataacgctga ccgttccggt tttcgctccg agcaaatagg ttgtaaggta aaccgccata
     2521 atcgtgaacg aaaccggcac aggtgtaaac ggaagcggaa ccgaaagagg tccgagcact
     2581 gatattatcg ctgccataag ggcaatttta actgtttcaa ttattctctc atgtcctgtt
     2641 ttcgcttttg cactcataaa ttatacctcc ggagccggtt ctgacggctg gaactttctc
     2701 ttaataagct caaatataca cttccacatt ttcttgaaag cctcaaagaa tgtaacgtga
     2761 tatctcgaat cgcaattcat attctcggta acgagaggag tgatctcatt cgcatccttg
     2821 tcataaagca tatactgcgg cagctccggg tcgctgaaca ccgtaaaatc actgccgtta
     2881 tttacaattg cgtcaatcaa gcggttaacg gctttcggga aagtcttgtg tattatgttc
     2941 ttaacaaacc atgtcttttc ggggaaaaga caggttgatg cgtcaatctg aaggtcgggt
     3001 gaaatatact tatatgtgcc gtcaagcttt gctttctcaa tataattgtc atcaaagccc
     3061 tgcatgagcg ttgctgttgt tgcgcccttt gatgcgtcat aaacactgca aatattatcg
     3121 gttatcatat ccgcttctgt agttacggga tatgtctgat agccgtactt tgtaatgttt
     3181 gcaacattaa cgccctcttc ggcgaacttc ttgagaagct cgtttgactt aacctgtacg
     3241 ttatagtgat aattgtcgat tatgctgata agatcggcat atttttcctt aacgccctcg
     3301 ccgtggaata ccgtgtcctt ggctctctca tagtcacggt cattgaccat gctccaatag
     3361 ccggggaacg tgccgtagga gttcataagc gcttgcggca taaattcaag atagtttttc
     3421 ttgagaacat tattgatcgc cgcacaggtg atgtccaagc cgtaggtttt attgaacacg
     3481 gttacaaagg actgaatcaa ctgattggtg acatcatcct cggagagctg aatgtcatac
     3541 ataaatctgt caactccgtc gggatcaata tacatattgc ctgcgaagca tctgctgcac
     3601 tgtgttgcac cgtcaagcgc acttgcataa agaatatagt ccgaaatata ttcggcattg
     3661 tatttctcca tgtaagccat agtgacgcaa gagccgagac agcggccgct cagcgcaacc
     3721 ttgtcggcgc cggtgacctc acgcacgtcc tcgatatacc gatgcattat ttctgcattc
     3781 tcgtaagggt caagtctcca gtcatagtta aacgtgaacc tctgagtcgg atactttccg
     3841 tgtgactttt taccgttaag ctttgcttta ttccatgtcc atctaagata aacgccgttc
     3901 tgcgtctttc cgtgctcgtc aaggcttatc gttgaatata tctcgctgag ggcatcaccg
     3961 agagcattgc cgaattcctc ccatttctga gtaagaacag ccttggcaaa aattccgatg
     4021 ttttctttaa cggtcttttc aacaaaaccg tctggaatct taacgggata aacctcctgc
     4081 tcctcgccgt tttcgtcctt aacaaacaga ggcatacctg aaccctgaac ataaattagc
     4141 ggaatgtctg ttccgtcaga cgcagcagcc gttcccggaa aatcaatgaa aataaatgcg
     4201 gaaataacga cggaaatgca gagaataacg gaaattattc ttttaaataa tttgctcaat
     4261 ttatttcacc tgtttcaaat ttaaatgttt gtatttgctt tttggtactc gtcaacagtc
     4321 agtgagttct ctgtttcaac gtgagagaag aaaaactttt ctctctcggg agagttgata
     4381 accgttacgg gcttaatctg attgagaacg acgccctttg ctctgagcga ctctctgtaa
     4441 tcccaatagc tcttttcttt aagaataagg atttccggct cgctgagcat accccagcgt
     4501 ctgtatttga aatatttctc gttaacgccc ttgatctcct catcaagaat ttcaatcatt
     4561 tttctgcgag tgtcttcatc aacggtcttt tccggctcaa caaacatgca atagcgaggc
     4621 ggcttggttg aataatcggg atagaagctg aagcccttaa agcctacgcc ggctttttcg
     4681 gctgtctttg cggcggaata ctcaaccatc tgagttgttg ttttttcatt ggcaacattc
     4741 atgctgagat tctgtctgta aagaagctca actctcgggg tgttgttata catctttgta
     4801 acatgaacaa cgtcctcgat cttatatctg taaagaccgg agaaatttga aacaacaagc
     4861 tcataatcct tgccgacttc aatctcattg ataagaagcg gcttaacgtc gtcttttgcg
     4921 tgatcctcgc cctcctcgat cggaaggaac tcaaagaata tgcaatgtgg aagaagaacg
     4981 gaatcattaa cattaagctc aaccggtgcg gcaaagaagc cttcggccgc agcatagccc
     5041 atgttgtgaa gcggaagatc gccgaccgat ttacgaagct tgtcaatata aacctggagg
     5101 cttgaaccca tcataccgta gccccaaaca agcttcggcc agattctcgg tgcgataggc
     5161 gtatcgaaac ctttttcgaa ttcttttcta agctctgccg ctcttgaagg attgggcttg
     5221 aacatctttg aatacttggc tctcagctcc ggagtgcact tgatattcgg gttaattatt
     5281 cccttttcaa tatcgtcgca gagcatctcc cagttctcct caaggtagtc aaacatggtt
     5341 gtaagaagcg tgataacaag agaaccgaga tatgttactt ttctgttttc aagagcaaaa
     5401 cgaagctgga gatacgaagt atcaataagc tcctcgtgct cggggcaaag aagtgaatag
     5461 ggcgatgtgc agaaaaatct tgtaatcggt ttaagatatg taagcggaac ctgtcctgct
     5521 ccgttacaac gcttgttatc gtcaagatta tgtcccgaga gaaccaaaac aagcggaccc
     5581 atctggttag gcattctgat gcccttgttc tttttgagcc aatgagcggc aacggcaacc
     5641 gaaaccgaaa atccgataca ctgcattttc caaaggtcgt tgataccctt tggaagaatt
     5701 ttcggcttgc cgaccgaacc cgacgacgag caatatctga tatttctgcc cgagaacatg
     5761 agtctgtcct ctttattgtt tatcattctg tcaacatacg gctcataatc ggcgtaagtt
     5821 gtcagaggaa caatacgctg ataatcctcg attgatttaa cctcaccgag acagagcttt
     5881 ttaccaagct cacaattttt atttcttctt acgatcttct tcaaaacgct tttctgcgcc
     5941 ttgagaggat cctttgtgaa atgatttatc tcccagagag aaacgtctcc gagaaaaatt
     6001 ccgaaatctg aaaaaggcac ttttttctac ctcccgctgt tttttattta tcacggaaat
     6061 atgcggcact gctcaatctc aagccaaggc ttcccctcaa ggcgaaggca ctccgggctt
     6121 gcttgactgt tgattaaagc agaaagccta ccaagctttc cgtagaatac gtttattatt
     6181 ctatcttata accnnnnnnn nnnccgctgt aatgtcaaca aacgacatta ggcaattaca
     6241 ttttctttgt tttttcatat gccgcaataa cggcattcat tgcatttgaa cggaatgcac
     6301 cgtcctcaag agctttaacg cccgcaatcg ttgtgccgcc cggactgcaa acctcgtcct
     6361 tgagcttgcc cgggtgcttg ccgctctcaa taaccgcctt agcggagccg agaacggttt
     6421 cggcagcata cagcattgcc ttgtctctcg gaagtccgca gcttaccgcg ccgtcggcaa
     6481 gagcctcaat gaacatataa acgaatgcag gaccgcagcc ggaaatcgca cagccggcgt
     6541 caatgagttt ttcgtcaatc caatcaacta tacctgcgca tttcattatc gaatcgaact
     6601 cgtccctgtt tctttcatcg acctcatagc cgctcgcaag aacaacgcct tcgccgacag
     6661 atacaggcat attcggcata attcttataa tatgctttgc ctttgaaaga gacttgattt
     6721 tttcaatcgc aacgcctgcc gccattgaaa cgagcacgca atcttctctg ctttcgagaa
     6781 tatcgcttat ctcggcaaat gccttgtcaa tgacctgcgg ctttactccc atgaatataa
     6841 agtcacattc ctttgctatt gtcacactgt cggaaacgtg aacgccaagc tcctttgaaa
     6901 gagcgtcggt tttctcggca aaatgatcgc agatgtaaat gctgtccgaa tagcctgcat
     6961 ttgccgccgc cttaacaaga gcgccgccca tatttccgca gcctatgaat cctatttcag
     7021 gcataatttt tccctcctta tcttatttgt ccgttaccct tgacaatata tttatatgtt
     7081 gtcagctcgc aaagacccat tggtcctctt gcgtgaagct tctgagttga aatacccatt
     7141 tcgcagccga ggccgaagca gccgccgtcc gtaaatcgtg tgcttgcgtt gacataaacc
     7201 gccgcagaat caacatctcg agtaaattcc gccgcatggg cgtcattgtt agttattata
     7261 gcgtcgctgt gctgagttga atgttcgctt atatgcctga cggcttcctc aacgctgtca
     7321 acgatgccga cagcaagaat ataattgaga aattccgtgt caaaatcctt ttcaccggcc
     7381 ttggttccgt caataatcct tgccgctctc tcgtcaaggc gaagctcaac ggggttttct
     7441 tttctgtcgt caaccagtct cttcttgagc atcggaagaa atttatctgc aatcttgctg
     7501 ttgacgagca aaacctcctc tgcattgcaa accgacggtc tgcttgtctt tgcgttttcg
     7561 ataatattga gcgccatatc aaggtcggca tcatcgtcaa cataaatatg gcaaataccc
     7621 gtgcctgtct gaataacggg aacagttgcg ttctcaacac aagccttgat aagacctgct
     7681 ccgcctctcg ggatcaaaag atcaatatat ccgtcggcag tcataagctc cgttgagctt
     7741 tgacgtgtcg tgtcctgaac catatttata aagttttcac ataagccgag ctttttaagt
     7801 ccgcttctga gcgcattaac tattgcgttg gcacttctga aagcctcttt accgcctctt
     7861 agaacgcaga cgtttccgct cttgagcgca agtgcggcgg catcggatga aacattcggt
     7921 ctgctctcat agattatggc aacaacaccc atgggtacgg ctgttttttc aacgataagt
     7981 ccgtttgaaa gctccacacg gctgagcaca cggcctaccg gatcgggaag cttgacaaca
     8041 tctcttattc cgtcagccat gccctttata cggtcttcgt caaggctgag cctgtcgatc
     8101 ataacgtcgg taatcttacc tcttgccgct tcaacgtcct ttttgttttc ttcaagaata
     8161 gctgcacagt tttcccggag tgcgtctgcc atggcaagca aagcgttgtt cttgtcctct
     8221 gagccgaact ttgcaagctc actctttgcg gccttcgctt gctttaaaat atcaagtgtt
     8281 gtcataatat catcaccttt ttataaacct tgtgcccacg gcttttccgt caacgatatc
     8341 gtaaagaata tcgggctttt cgccgttggc aattatcata tcgcaattct tttccgtgca
     8401 aatctgagcg gcatggagtt ttgttctcat tccgcccgtg ccgaaagcag aaccttcatc
     8461 gccgccgaga gcgagaatat cgtcatttat ttccgaaacg acggaaatga gctttgcgtt
     8521 cgggtcagtg tgaggatcgg cggtgaaaag tccgtcgata tccgaaagaa gaatgagtaa
     8581 atcggcactg acgttgaccg caaccatagc cgaaagcgta tcattgtcgc cgactttgat
     8641 ttcctcggtt gcaacagtgt cgttctcgtt tataatagga agcgccttaa actcaaggag
     8701 cttatccatt gtatttttaa aattgatatg tctttcctcg ttttcaaaat ccgagcctgt
     8761 cataagaagc tgagccacgg tgtgattata ctccgaaaag agcttatcgt aggtatacat
     8821 cagctcacat tgaccgactg cagcggcggc ctgctttgcg gcaacgtcac gaggtcgttc
     8881 gcttaactgg agcttacctg cgcccatacc gatggcgccc gaagtaacaa gtataatatc
     8941 atgtcccgca tttttgagat cgctcatgac cttgcaaagc tcctcaacac gtcttatatt
     9001 gataagaccg ctcggatgcg caagcgttga tgtgccgacc ttgacaacaa ttctcatgtc
     9061 gtaaacctcc gttaaggtaa tattactgca ttatcgcttt attttatcac ataactctct
     9121 attatgcaag aaaaaacaca cccgaaaaat aaatttcagg tgtgtttaaa cgtcaatata
     9181 aattatttaa ccgtatatgt cagtgtttgc tgatatttcc agccgtctgt cggagttttg
     9241 atgtaaagct tgaactcatt ttttccggtg tgacggggtt taacggtgat attgtaggtg
     9301 tatgtcagca agctttcgtt aaacgttgcc gccgaggtgt tgagggttgt ggtcattccg
     9361 tcctcatact caatgcgaac cttggttact cccgagcctg tgacaatttt acagtcggca
     9421 taactgtcgt tatccttatt cgatgaaatt tccgctgact tcaatgtctg atccgaataa
     9481 agagttgtaa ccgtaaattt atgtttaatt tcagaccatt caagaccgta ttttgagcga
     9541 acctgtaaat tagtgccggg tgtcagcctt gtatgaaccg tccatacctc atatgcaagg
     9601 tcggcggaca tatcgtttac cctttgtccg ttcgcactgt aagattcaat gctgacattg
     9661 cctgcatgac ggttaaaggt tctcgttgcg cccgtttctt caatgacctg aattttatcc
     9721 gctctgtcgt caacatggaa tttgtaatca tttatactgc tgttcgatgg ggtgtaattt
     9781 acagccgaaa ccatataaag agggactgca ttagggatat aaaagatagc tccgggtttt
     9841 gtatatatat cagaaaattc ttttggcata taaattttag tattatcact gaatccacaa
     9901 tcaatcaaat cagagggatc gggaatgccg caaattataa ctttttcaag atttttgcaa
     9961 ctgtaaaaag atgaataaca tttaacatta ctgccccaaa taatctcttt aagactcgga
    10021 cagttatcaa agaccatgta acctattgtc agtttgttat tgctttttct tgtgaaatca
    10081 atcttttcaa gcgcagtaca gccctcaaaa gcacggtcat caacttctgt tgcgatattg
    10141 tcgggagtaa aatatacctc cttgagtgaa gagcatcccg aaaacatgcc attgcagacc
    10201 tcgcttgcaa cataagctgt tttcagattg tcacaaccgg aaaaagcact tccgcgataa
    10261 cctgatctat cctgctcaaa aacaacgttt gacggaacat acagcgtctc tatctcaatt
    10321 cccgagaaag cgtcgccgcg aagcgtaatt tttttgcccg aaccgtcatc attcaaaata
    10381 agattataaa cagttgcgta tgaaaaatcc tcatcataaa cggaagcacc cggattaaca
    10441 ataaccttat caaaggatct gccgaaggta tagcttgttg ttatcatgtc gtcaattgtc
    10501 aggatgtttt cgcccgtaga attatcgcaa tttatcgtaa caaatttaaa gccttcccgt
    10561 ttcgcatatc tctcggcttc cgttccgcta tggccgaata ttacaaaacc cgtgagtctg
    10621 tttccgtaag agctatagcc gaatgcatta tcaccgatcg acttgaccga agccggaata
    10681 tatactttcc aaaaaaaagg caaatcattt acaaatgcct cactgcctat gctttcaagt
    10741 ccctcattga gaataagatt atcaaagtta ttctcagtgt atctgccgtc ggggatttcc
    10801 tttgtaccct cgggaataac gcaataggca ccgtatttct ccgcttctgt tacgtcgccg
    10861 taaaaatttt gcgtctcaat cctgtctgcg gcataagcgt ttgccgaaag aagcatacat
    10921 acggcaataa ctaccgccgc cgttctcatc caaaaaattt tcataatcat tcctcctcat
    10981 atttatcttt aatttcatta tagccaaaag caggaatttg tcaacaaaaa ttcctgcttt
    11041 ataagtctat tctatgtgta tttagttctg ctttcttacg attttgtatt cgtcatacgg
    11101 gtcgtaatac gggcagttat agaacgatgc actgacgaat ttcatgtatt cgtcctcgtc
    11161 aagctccatt tcgcaataat aagtctcgct ctcctcgtca tagacgaagt tgttgcaatc
    11221 ctcacagttt gtaaccttca taaatatcta cgtcctttat tttctgccct gcgccttaat
    11281 aagattttga taaaatctta ccgcacccgg caggcagttg tattcaatac attcgttgag
    11341 tccgtgcatt ccctgcatct gatcggggcc gtaaataaca ggcgcaaaac ggatacagtt
    11401 tttacaaatc ggctgataga aatgagcgtc ggttgcgccc gtcataacat aagggctcga
    11461 cgcaataccc gggaatgtct cggcgataac gccctcaaaa agcttgaacg cttcaccgtt
    11521 gatgtcaacg ggcttcgaat agtcattgga atgtaaaacc tcggtatcaa gtccgtgctt
    11581 ttttgcaagc tttgtgataa tatcaaggct ttccttttcg ccctgatgcg gaatgaagcg
    11641 catatttgcg ccgagtgttg cctcctgcgg aataacgtta taggcgtcgg aaccggactg
    11701 aactgtgaac gctatcgtag ttttcagcat tgcaccggcc tgggcgctga tagagggcag
    11761 agccgcaata ataagcggtt taaaaagcca catgttgcca agaagaaatc ttaagccgaa
    11821 tgaagaagcg taaggcgcaa gggtcgtaaa cattgcctca acctccggga gaagtctctt
    11881 tttaaacggt gagtgttttt caacgtcatc aacgaacgaa gagagtcttg cgataggcgt
    11941 gttctttgtt ggagcactcg catgtcctcc cgtgctgtga gcggtgaatt tgacgtccgc
    12001 cttgcctttt tcaaaaacgc cgaccattgc aaaattaccg ttaacaccgc cgatcggatt
    12061 tgtgataata cagccgccct cgtcgcagac aaggaaaagc tcaattccgc gccgtttcag
    12121 ctcatttacg attttcggcg caccgtcacc tgcccattcc tctgtgcatg acgaagcaag
    12181 atatacgtca acaggcggcg tgtagccctc tttcagcaat tcttcaaccg cttcaaaaaa
    12241 tgccataacg gagcattttg tatctgcggc gcctctgccc caaaccttgc cgtcctcaat
    12301 atcaccggag aaaggctcgt gcgaccattc gccctcggca ggcaccacgt cctggtggct
    12361 catgagaagt atcggctttt cggagctttc acccttccaa tagaaaagca aatttccgtc
    12421 gatctctgtc ttttcaagct tttcgtggac aagcggaaag agcttttcaa gcaccttgtg
    12481 aaaaccgagg aatttatcaa cctcatgtac attcgcgtgc gaggttgtat cgtacttaat
    12541 catttcagag agcttttcgg caaggcgaag cgattcctgc tcgtcctcat tcgccttgta
    12601 atcggatttt ttcttaggca tgagaagcgt gcgaattaaa gcgatcagca ataaaagcac
    12661 tataacggcc ggtataataa gcaaatactt cattttcaag cctccttgct ttttttataa
    12721 ataaagtagg aaattcctat ggctattgcg cctgcgggaa taatcataaa actcgtcgag
    12781 cctacaagcg aaggaacaag catgaacagc acgctcatca caacgagcgg agcaacggca
    12841 atgctcatgt tcttgcgata atatttataa gccattgcac cgaaaagagc cggaacaacg
    12901 ttatcaaacg caggctgaag cgtcgggctt tggagaagcg gctgaagcgg agtaagcgcg
    12961 agca
//
LOCUS       DAWBSW010000036        12852 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1052_length_12852_cov_2.677190, whole genome shotgun sequence.
ACCESSION   DAWBSW010000036 DAWBSW010000000
VERSION     DAWBSW010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12852)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_12852_cov_2.677190"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3516..3525
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4456..4465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3380 a         2765 c         3038 g         3649 t
ORIGIN      
        1 gtcttttggt gccggtgacc ggacttgaac cggtacgatg ttgccaccga gggattttaa
       61 gtcccttgcg tctgcctatt ccgccacacc ggcttatgtg cggcaactgt tacattatac
      121 acaacagttg ccgttttgtc aacataacaa taatctaatt atttatcagc cgttgattat
      181 ttcgtgaagg tgtgccttca atgcatcaag gcgagcgtca ttttctctgc tcttgtcctg
      241 aatagaatag taaaccttga gcttcggctc tgttcctgac ggtctgatag caagccatga
      301 gccgtcctca aaaatatact tgagaacatt ctccttcgga aggtctgcaa taccctttga
      361 gaagtcgtaa accttcttaa tgttgtcgaa gaatttgctg ccgttttcac ggaactcggt
      421 catgaggctc tgaatcttct gtgcaccctc gatgcccttg agaacgaagg tgtcaagaga
      481 atcaagataa taaccgtatt tatcgtagat atcattaagt gcgtcaataa gggtcttgcc
      541 ctgattgtaa taataagccg ccatctggca tacaagcatt gatgaaacaa cagcgtcctt
      601 atcccttgca tgtgtgccga cgaggtagcc gtagctctcc tcatagccga taatgaattc
      661 tctgtcaccg gacttttcgt atctgtttat ctgatcgccg atgtatttaa agcctgtgag
      721 cgtttcggca acctgaagtc cgaagcttct tgcaatgttt gcaccaagct cacttgtaac
      781 gattgtcttg acaagagttg atttgctgtt gagcgaatcg ttcttcatgt tgagcacaaa
      841 gttaacgaga agcgcaccca tctgattacc tgtgataaga acgtatttgc cgtcgtgctt
      901 tacgcctgcg ccgatacggt cacagtcggg atcggttccg aaaacaaggt cggcatcttc
      961 acgctcagcc tgcttgagag cgagagtaag agcgtccttc tcctcgggat tcggggaacg
     1021 aacggttgag aaatttccgt cgggaagctc ctgctcctta acgacagaga tattcatacc
     1081 cttgagaatt tttctgacgg ggatattgcc cgtgccgtga aggggagtgt aaacgatctt
     1141 gatgttcgaa ggctcctcgt aaagtgactg cttcctgact tccttgagaa atgcgtcaag
     1201 cacctctgag ccgattgact gataaagtcc tttctttgca gcttcttcaa gatccatgtg
     1261 cggaaccgcc ttgagatcct caatcgcatt tacatatcct ataaccttgt ttgcctgatc
     1321 ggggcaaagc tggcagcctg tttcgtcata aatcttgtag ccgttatatt ccttcgggtt
     1381 gtggctggcg gtaacaaccg agcccgacgt gcagccgaga tatcttacgg tgaacgaaag
     1441 aaccggagtc ggcatgagtg tatcgtaaag ataaacctta attccgttag cgcagagaac
     1501 ctctgcggcg tatatggcaa actgtcttga attgtttctt gaatcgtggg cgatcgcaac
     1561 gctcttttcg ccgtcaaact cggctttaag ataatccgca agaccctgag tagccttgct
     1621 tacggtatat ttgttcattc tgttggcgcc tgcgcccatg attcctctca gtccgcctgt
     1681 gccgaactcg agatccttat aaaatctgtc ctcaatttct ttttcatcgg tgattgcgag
     1741 aagctctgct tttgtatctt cgtcaaagcc gagccactgc tgatattttt ctttgtagtc
     1801 cataacgatg ctcctttaca taagaaaatt taattttccc tcaaatttct ccacataaat
     1861 catatcacaa atgcaccgct tagtcaacgg acaaagggca aaatataata tattttatgc
     1921 gaaaaaaaac tcggatatta agtgacaata aaatatttat ttatgaaaaa atccgaccct
     1981 cgtttgaagg tcggataatt ttcggtctgt cactgtttgt ttttctttct ctttttcttc
     2041 acgattaaaa ttacaagtgc cgcaacggct gcgatgagca cgatataggg gctttcgccg
     2101 agaataaata tgccgaggct tctgaaaaac tgtcctatat tgtaaatact ctcgaagaaa
     2161 ttctctttga cctgcgagcc gaagccggtc ggagttttct ttacccgttc aacctcggaa
     2221 atacttatca ggatttcgga atatgcaatg cgctgatcgt agtttttctt ttgcactctg
     2281 agcgattcaa gctcacctct gacgcttgcg agtctttcct gaacgctcat tgtgtcctga
     2341 actgtttcgc attttgcgag gatgccgagc aaggcctttt cttccgtttc gagtgctttt
     2401 atttggctct cggtattggt gtaattatcc gtcacgtcca aggtttcaat gttctttgat
     2461 gtaatcgttc ccgttttttc aagaaaatca atgaattctt caagcttttc ggcagggatg
     2521 caaacgtttg tgtcaaccgt cagataaccg tcgtacttgc tttgattgag cgacgaggtg
     2581 tagcccttga gggagcttat ctgtgagttg atatttttaa tcagctcgtc ggcatttttg
     2641 gtttccaacc tgacggaagc cttttttatc agcttctgcg aagatgctga tgcagtgttc
     2701 gcattgccgt atgatccggc actttcgtta gcgttgcctg cgccgtttga attgttatag
     2761 ctgtatgtgc cgctgtcgcc gatctcaatc gaacttttgc tttggaaaag tgacgggcct
     2821 actccgtaaa ctccgcatat cagcactgta acagcgaggc aggcggcaac aattgccgta
     2881 atcttttttc cttttttgtg ctttgccgaa gggacaggct tcagcttgga aatattgtct
     2941 ttaagcgaat cgggagcatg aagctcgtcg acttctctga tgtagtccgt tttattcatc
     3001 gtcaaaacct ccgatcattt cttcctttag catagctctg ccacggctga gccatgaaag
     3061 gacggtgttc ggctttgcgt cgagaatttt gccgattttc ttggcggtat agccctcgta
     3121 atagtgaagg taaactgcgt tgcggtagtt ttcgggcaac gctctgaccg cttcaaggac
     3181 agaatcctct ttctgcgccg tgttatttgg caaaggagac tcgcaaagca cgattcttga
     3241 gttttttctc agctcgtcac ggcaaaggtt tattgtcaca cggataagcc atgctttcag
     3301 atgctcctcg cctctgaaat gtttttcact ttcaagaagc ttgacaaata cagcctgcgt
     3361 tatatcttcc gcatcggcag gtctcgccga attatgtacc gcaacacggt agaccgtatc
     3421 cgtaaactga gaaaatgcgt aggggaaagc gtcctttccc gacataaaaa atatcatgat
     3481 attgcctcct ttcatctata acacgttttg acaatnnnnn nnnnnattgc aagggggatt
     3541 attttttata aaagatttat aagctcggca actgtgaaat cattatacat ggaagcgtcg
     3601 agaatatcaa ggtttatctg cgaatattcg acctccttcg agtatccgta aagaatacgc
     3661 acacgcctgt caaagctcag ctcggggacg ctgtcgtaga gcctttcggt gatgatacac
     3721 gcaaaaatgc cgtacttgat ctttgtaagc aggtcgaggt caaagcaagc cgtcatcatg
     3781 taccgcatga ggtaatattt gaaaagtcgg attatatcgt cggaatatat ttcactgttg
     3841 cggtaaatcg ctttttcgtc cgagagggaa atgagctttt ctctgcgctc atcgctgata
     3901 tattccatcc tttcaagaac cttgacgcac tcggaaaact caacggtgct gtcgtcgccg
     3961 agcaagtcaa ccttgtcaat atcgctttga aattctgcgg cggagaaaag cacggcggaa
     4021 agcttcattt taaggtcaaa gctttcgttg tcgagaatcg caaaaaactc ctcacgcttt
     4081 tctgtcagca ggttatatat tctgtccggg ctttcaaccg tcctgtcaag gctcacgtcc
     4141 gtttcgggag acagaagaat tcttgccgct tcggggcagc cgagcccgag acccgtctca
     4201 tgaatttcgc cgtaatcgtc gaagtatctc ggaaaaagcc ggcaggtgtc gcaaagcgcc
     4261 gcttcgccct tctcaagctg aattcggcaa aggttgtttt cattgagcag ggggcagcgg
     4321 ccgttctcaa gagagaaaat atatccgtcc tcgtccgtcg tcaggtgcct gtcaaaatat
     4381 tgcttcaagc cgccactttc atttttgtaa ttctcagccg aagcatcgtc gatctcaacg
     4441 tcccagccgg cacagnnnnn nnnnnttttt tcatcacttg ttcttccgtt ccttaacagt
     4501 aaaaacgctg ttctttttaa aattgttaat aaaattgtaa caccttgcaa ttaaatgtca
     4561 agtataatat atatcgtaat atcgtaaacc gatacggtta tcaaagcctg aaagcgggtt
     4621 aattataaat gaacagtttc atgcaaaaat tatataactt catgtgcggc aggtacgggc
     4681 ttgacaagct gacgggattt ttgattttat taaatcttct tctcaacggt ataggctcgt
     4741 ttatgcgtaa tcgaatagct tattatatct tctatgttct ggctcttgct gttttcgtct
     4801 ttgcggtttt ccgtgtgctg tcgaaaaata tcgaaaagcg ccgccgcgag ggtgaatggt
     4861 ttgacaattt gcttgtcaga accaattata ccgagagctt tcgaaagatg aagcgctggt
     4921 tcaaaaggca gggcttgaga tttaagtatt tcaaaacaca caggttcaga gtttgtccga
     4981 attgcggtga aaatctgcgc ctttcaaaaa agcgcggcaa gcggaacata acctgcccga
     5041 tttgcggaaa taaattcagt attcacattt tgttctgagg aagtggactt ttgcttgggt
     5101 atgtcagggc ttacaggccc gaaatgaaat tcaaggatta tgatatgtac aagggcgttt
     5161 attgctcgct ttgcaagtcg ataggcaagc gttacggctt gcttgcaagg cttacactgt
     5221 cttatgattt cacttttctt gcaattctgc gaatggcggt cagggaaaac agagtgtgca
     5281 tgaaaaaatc gcactgctcg tttaatcccg taaaaagatg ctacgattgc tcgggcagca
     5341 gcgttgatat agcatacacc gccgatgttt ccatgctaac gttttattat aaaatcaggg
     5401 ataacatcgc agacagtaaa tttttcaaaa aattgctttg caaaatgctg atgccctatg
     5461 ccaatcatat atataaaaag gcgaagaaaa acaatcccgt gatcgccgaa aagctgggaa
     5521 agctcattaa ggagcaggca gaggcggaga aaagaaatgc cggcgttgac gaggcggcgg
     5581 acgcaagcgc aaaaatgctt gccgagatgg tctcgaccga cattgaagcg tcggacaaca
     5641 cggcgctgaa caggctcggc tatttccttg gccgctgggt ttatcttatt gacgcggttg
     5701 acgactgcgg ggacgatatc agaacgggaa gcttcaatcc gctgaaaaag cgatataata
     5761 tgccggattt caaggaagcc tgtgagagaa tgttgtcgct tactgtcgga gaagcaataa
     5821 ataacttgaa taagctggaa attttcagat ttcgtgatat aatagataac gtgcttgttt
     5881 acggaacgga atgttccgcc aaaaaagtaa tttacaagga ggttgaatgt tgtgaaaaat
     5941 ccgtatgaag tgctcggcgt tacatcgtcg gcaagcatgg acgagataaa aagagcatac
     6001 cgcgacaagg ctcgccgata cagcgaaaat caagcgaaaa tggacgagtt gaacgatgct
     6061 tatgatttta taatcaacaa tttcggcagc aacaaagcaa atacctccta tgccgattcg
     6121 ggctacggaa ccggcgcagc aggcggacag acctcggaat tcggcgatat aagagccaag
     6181 ctcaacagcg gcagaataga cgatgcagaa atgctgctcg acggaatacc ttatcagcgc
     6241 agaaatgccg aatggtacta tctcaaggga acgattcagc atagaaaagg ctggctggag
     6301 agtgcacttg aaaactttcg gaaagccaag gagatggaac ccggcaattc ggagtatgaa
     6361 gccgcattca atcagcttga gcgtgagtcg aagggtgatt tcagaaggaa caggcagaat
     6421 gaagataacg acggttgctg cagcccgtgc aatatctgtt cgtcgctcat gtgcgccgat
     6481 tgctgctgcg aatgtatggg cggcgacctt ataagatgct gttgacggtg atgatatgaa
     6541 aaatacggta aaaacggctc tgggcggaat gtgcatcgga ctttcggcgg cgctgatgat
     6601 gctctcctca gtcattcctt ttttgacata tgcgattcct gccatggcgg cgatactcgt
     6661 aatgtttatg agaattgaat gtgactggaa atggggactc ggcgtctatt tcggcactgc
     6721 gatagtcagc gcacttgttg tccccgagaa ggaagcggtg gctatctaca tagcgctgat
     6781 aggctattat ccgctgttaa aaattctctt tgacaagctg ccgaaaattg tcggctatat
     6841 agtaaaggca gttttttttg tcggggtaac ggttgcgacg tattatataa taatgcacgt
     6901 tttcggcata tcgaccgagc ttttggagga tacgcaggga tttatgattc cgttgctcgt
     6961 cgtgctcgga cttgcggcat tcataatgta tgacattgca attgaaaagc ttgaaatagc
     7021 atattacaga aaatggcaga agcaattcag aaagctattt aagaagcact gatttttcgg
     7081 tgcttctttt catatgtacc gacttgtccg catagtattt cttcgggagg aatgaacaat
     7141 gaggaaatac ctgatacttt gttcggtgct ggtggcggcg atgataattc tgccgctgac
     7201 ggcaatggaa aagccgaaaa aaacggtaat tgtcagcaat aagccgtccg taaatacgga
     7261 aagcgacatt ataagcgtta tgaaaagtga gagcggtaag gtcgaaaaaa ccgacataaa
     7321 ggaatacacc gtcggagcac tggcggcgga aatgaatctt gaaagtcacg atgaagcact
     7381 caaggcgcag gcggttgcgt gctataccta tgcgctttac tgcaaggaaa aggcaaacaa
     7441 ggacgacttg aacggagcgg atatatccga cgattcaaaa acgcatcaag gctatctcaa
     7501 caatgaggca agaaagaaaa aatggggaga caagtatgac agctacgaaa aaaaggcgga
     7561 aaggctcgtt gatgaggtgc tcgggaaaag gatgacttat gacgggaagc cgattcttgc
     7621 ggcatatcat gatatatgca gcggcaaaac cgagagcgca aaaaccgttt ggggcgagga
     7681 tatcgcttat cttcaatcag tcacaagcga cggcgacagg ctttcgcccg actatgaaac
     7741 aacgatgggc tatgacaagg ataaattcct ttccgcaatc aggataattg acggggtcga
     7801 gctgcccgag aacgccgaaa aatggatcgg caagacggat aaaaccgaca cgggatttgt
     7861 gcgttccgtt gaaataggag gtacaaaggt tgacggtaac gatataaaaa aagcactcgg
     7921 actgccgagc agaaatttca cgataagctg caagaacggc aggtttacgg tcaaaatcat
     7981 cggcaacggt cacatggtcg gcatgagtca gtacggagcg gactatatgg ctcgtcaggg
     8041 ctcggattgg caggagatat tggagcatta ctatacaggc ataaaaatcg agtaaaaata
     8101 ataacatttt ccatgaaaat aatttgtaaa aactcttgct tttttgaaag aatatgttac
     8161 aataaagtca aatcaaccag caaggaggta acaacaatgg cttttaagat tacagacgac
     8221 tgtatcgctt gcggtgcttg cgaggctgag tgcccggtag gcgctatttc cgaaggcgac
     8281 ggcaagtatg agatcgacgc tgatgcttgt gcatcctgcg gcgcttgtgc cgatgtttgc
     8341 ccggtgggag cacctgtaga ggaataatta aagctttgtg atgtttaagc gaagcggctg
     8401 aaatcattcc acaatatgat tcgcttcaaa aaacataata aaagagcaaa gacgctgtgc
     8461 atatcaaaaa ggtatgcaca gcgttttctc attttttatt tcttataaat cctgttcgtt
     8521 acgaatgttg tgatgcccat ttcgtaatat ttccgcatgg tttccttatc gtcaactgtc
     8581 caacaggcga ggggtattcc ggcgtctctg agctgcttga tcttcttctc gttattgatc
     8641 ctctgatgcc cgttgaagct gacgccgatt gtcggatcgt caagacactg tttaagagaa
     8701 tccttgtcga gcttcgagac gagtgcatag agttctatct tgccattgcg ctctctgacc
     8761 ttgtttaagg cgtttcggtc aaacgagatt accgagcagg actcggtgaa gccgtttttg
     8821 tcgatgattt gcagcagtgt gtcaagtccc tcgtcggtat agtcctttat ttctatcatc
     8881 ggtttgacgt tgtttgcaag gcaagcctga agcatttggt caagggtagc gatttttaat
     8941 ccgccgtagt ttttgtgatt tgcgccgtta tcaatgtcgg cggttatcag atcatagtac
     9001 gtataggacg aaattttacc gctcttgtct gtcatgccct tgatgtcggc atcgtgagaa
     9061 acaacccaaa cgctgtcggc ggtttgtctt atgtcgattt caactgtgtc gaagccgtat
     9121 tctgcggctt ttttgattgc cgcaatggtg ttctcgggcg cctgacagga aaagcctctg
     9181 tgagcaatca ggttgatttt attctgatct gtttttttga catagatgct cgagagagca
     9241 atgggacttt tggccgtgta tcttttgtgc agagcgacgg catttgaaac aatcggcgcc
     9301 gatatcaccg caattacaat caatacggct aaaacgatgg gccatttttt tctttttata
     9361 atacgaaacg aacgcttaaa agcctcttta ttcataaaaa atcctccgaa atgaagttta
     9421 accgtaacta cagtttatct cattcggaga attatgtcaa tcatatattt gccgacggcg
     9481 gatatccttt tgaccgtcgg ctttatattt aaacggatat cttacgattg cggcatttct
     9541 gcggacagga tgtcaccgcg gatcaatatc cgttgacctt atttttaaca tcctgttaca
     9601 ttatatgcct ctaaagcggc tcgggtctca aaaaaatacg gtgagccgtc gctttttggc
     9661 agctcaccgt gagattaatc ttttttatcg cccgagtcct tgctctcttt atactcgtta
     9721 tatttttcgg aaacggtttc aatcatggat tccgtctttt ccttgacggt ttcggtggtt
     9781 tctttaaaga aacgcttgat tttgttcgag cttgttttcg ggaactcgct gtcacgtatc
     9841 ttgacctttg ttatctgctt gtagataggc agctcgacac atgccttttt aatgctcttg
     9901 agaacctcag agggtgtttt cttttcctca agatatacct cggcggtcag gctgctctcg
     9961 ttgccgtctt tgtccttttc gccgctgaca acaacctcat tgatatagtc tatatcctga
    10021 atatagccct cgatttcctc cgggcagaca tttttaccgt tgttgagaac gatgatgttc
    10081 ttctttctgc ctgagataac gagctgatcc ttttcattga tgtaaccgta gtcacccgtg
    10141 tagaaccaac cgtcctttag cacctcggcc gtcagctcgg gctgcttgta gtagccaatc
    10201 attacaatgt cgcccttgac gcatatttct ccgattccat cgtcgtttgg ctggtctatt
    10261 ctccattcaa tgcaggggag cttgatacct accgtcttga aatcattgta catatcatgg
    10321 ttggcgcata caagagggga gcactccgtg attccgtagc cgttgatgag gtcaagtccg
    10381 atatcgtcaa agaactttgc aacctcaggt cttatcggtg cgccgccgca tacgattttt
    10441 ttcagcttac ctccgaagcc ctcaagaata aatccgaaga actcacggcg cttgtcgatg
    10501 ccctgagcac gaagctcgtt gctctttttg acaagttcgt tgaactgctc ctccatgccc
    10561 tgctttttta tgtttttgat tatgcgcttg tacatcgact cggcaattgc gggcacaacg
    10621 taaacgtatg aaggcttata aataataagg tttttgagga ttgcaggcag actttcgttg
    10681 atgcagagcg ttgaatgatg gtggaaagaa acaagaatat cgctgaccgc ctcgtaagtg
    10741 tggtgatagg gcagcaggga aagacccgtg tcgtagaccg tggagaccat gagtccgtaa
    10801 tagacgctcg aaacaaggtt gtgctcggag agcataactc ccttggaaaa gcctgttgtg
    10861 cccgatgtgt aaacaagaag cttcagctca ttcggatcac tctgctcggc aagaaacgcc
    10921 gaagggtcaa gctcggcgcc cttttcaagt gcctttgaat aggaaaggaa cattccgtcg
    10981 tcatcttcct tgtcaaagac gataaaatgc tcgacacccg ggagcttatc aagattattt
    11041 ctgaaaaact cctcaaattt tccgtcgcag aaaacaattg tgcactcact gttgtttatg
    11101 acgtgaataa tatccgcttc cggcagctct ttgtcgagcg gaacaaaaac gccgttgccg
    11161 cgaagcgtcg taatatatgc cgttatccaa tcgtaggagt tctttccgat acaggcaaca
    11221 tgtcttttat catcggagat ttcgtggaga aatgcgccga gtttttttaa agtttcatag
    11281 aactccgaga aggtaatctc atggacgtcg tcatcaatct taaatttata tgctattttg
    11341 tcgcctgcgt cacggacggc aagctcaatc atctctttta cggacgaaaa aggctgaacc
    11401 tcgtaaagag gatagttaat ttttttagac atacttagta cctcccactg agaagaatat
    11461 acttttatta tcttaattat tgcctaaatt atcgcatttg aatataaaaa agtcaataga
    11521 cgaattgctg attatttgta aacaaaaaat gacttatatg taccttttta agttatttat
    11581 attttatttt cggcatacaa tttatctgag gtgattgtat ggatgaggca gtagcaagac
    11641 cgaaagaaaa gacagagcaa actgcgccgg cggagaagaa aggcagcccc gtcaatacgg
    11701 tgatgcttgt tcagcttgtt gtgtgtattc ttgccgttgc atcgatattt cttatcggca
    11761 ggctgagtcc gcagacattt gaatttatca ggaatgagta taacagaata atgtccgttg
    11821 acatgaatgc agaagaaatt gcgtcctcgg caaaaaaggt tatcaagaaa tcggagacgt
    11881 cggaagatga acagacggcg gagcgggagc aaacaagcaa aaaacagagt gtaaataccg
    11941 ttaaaagcgc cgccgacggc gaagctatgg ctgttatgtc gctttttaaa agtgacgaag
    12001 aaataaccgt tccggttcac ggagaaataa cctcggagta cggaaacaga accaatcccg
    12061 tttcgggcga atatttgatg cactcgggta ttgacatcgc agcggatctg ggaacggaaa
    12121 taagggcggc atacagcggt atcgtcagcg aggtcggcag caacagtgtc ggcggcaatt
    12181 acataagcgt tgttcaccgt gacggcagtg aaacgctcta ttgtcattgc tcggaaataa
    12241 ttgccgaaaa gggcgacgtt atccgtgccg gtgaggttat cgcgcttgtc ggcagcaccg
    12301 gcagaagcac ggggccgcat cttcattttg agattattgt tgacggcaaa acagaaaatc
    12361 cgttattata tctgccgcac gaaaacggtg aggtatgagg cttcgcatac ggggagtgcc
    12421 cgttataatt gattattatt tcatcgccgt tctgacgctg atgcttgtgg ttttcaaaaa
    12481 tgagaatatt ttgatgtgtt ttgttttttg tattctccac gagctcggac atctgacggc
    12541 gatgactttt ttcggtgaaa gggcaaaaag tgttactctc ggctatttcg gaatgaggat
    12601 tgactgcgga gcaagaatcc tgcctaaaat ccccgaaatt gtcattgcgg cggcaggtcc
    12661 tacggtcaat ttgattttaa tgctcttttg ccgtcttttt aagcttgatg aagcggcgca
    12721 gataaattta agtcttgcgg ttttcaatct tctgcccgtc actatgcttg acggcggaaa
    12781 aattctctca tcatttgtca cagagcggac aatacgtatt atcggtataa caatcggtat
    12841 tattctctcg tt
//
LOCUS       DAWBSW010000037        12773 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1060_length_12773_cov_2.703570, whole genome shotgun sequence.
ACCESSION   DAWBSW010000037 DAWBSW010000000
VERSION     DAWBSW010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12773)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1060_length_12773_cov_2.703570"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9724..9733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10340..10349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11494..11503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12323..12332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3591 a         2935 c         2958 g         3249 t
ORIGIN      
        1 tcgttttaat ttagttgctt tccctgcgac gatcaatacc aaatccggcc gaagaggaat
       61 atcaatatta accaccggaa gaaattgtaa ttaacttcaa ccttatacat tctaaggttg
      121 gcatccgctt catccatagc ataaataatt gtcgatccgg tgttgttcgc tctgattttt
      181 ccgtttgaat ccacacttac aatagagctg tttcccgact cataagcagc ataatcggaa
      241 ccgttgttta ccgaataagt atccttataa ttgagataga cccttttttc ttgataggat
      301 caagacataa actctgtccc aaactgctct cctttccttt tgttttctcg ggaaatttcg
      361 acctttaatt ctgtcttaat ttcttcccgt gttttcaata aatatatttc atttattttg
      421 gcggttgtaa tttgaattat accaattgta aaccctgtca gaaattattt aataacgcct
      481 aaaggcgaag tgcggatcgg gagaatctgc atttcgcctt tataaattat ataaatcttt
      541 ccctgcgaag catagattag gaaaaatccg aaccgaagcc tcacatcgga taaaaccggt
      601 ggataaaaga cccgatgcac ataccgatta ttatccgcat taccgacatt ttgccgacag
      661 ttaacatgaa tattttacct tttgaagttc aaaagcgcaa ccatgtgaca gcaaaagatt
      721 tttttctttt gttgtcacac cccctgtttt tttcaatttt gtatgaatgc acaaattgaa
      781 cttttttatt ttgcgttctt ttgtttgctt ttgtttccgt ttcggaacat ttcgctttgt
      841 cctttatgcc gtctttttta attacatctg cttgcaaagt gcatatcaaa tgacgccggc
      901 aatgtctgat tatcaatttt ctttgttatt ttctgcctaa agcaacttca tggatagtta
      961 ctttcgtttt atcggcgaca gcattgtttg ccgataattg ttttattctt gtttgctctg
     1021 tctgttttgt aacaaagtac acaaataatg cgacaatttt ttacatttga tttgaatata
     1081 ataatcaaaa acggcttgaa ttttactttt gtttttgata caatatataa tgtataaaaa
     1141 tataatacca ataactattt ctttgtttgt cgaggtgacg caatgaaaaa gaaaaaaaca
     1201 agtgtaaacg acgttcccct ttaccttttt catcagggtc agaatgccaa gacctatgat
     1261 tttctcggtt ctcaccgttc gggcagggac ggggttgtgt tccgtgtttg ggcgccccat
     1321 gcggcggcgg tcagcgtggt aggcgatttc aacgaatggg atattgaagc cgacccgatg
     1381 aagaaacttg aggacaacgc cgtttgggaa tgtaaaataa aaggaataaa gcagtttgat
     1441 aactacaaat actgcataac cactccgtcg ggcgagcttc gctacaaggc ggatccttac
     1501 ggattccatg ccgaaacacg gccggaaacc gcatcaaagt tctatgacat tgacggatat
     1561 gaatggaaag acgaaaaatg gatggccgaa agaaaatccg taaatattta tgaaagtccc
     1621 gtcaatattt acgaggttca tgcaggatca tggaaacagc atgaggacgg aaattttctt
     1681 tcctaccgtc agttagcaga cgaattgaca aaatatgtca aagatatggg ttatactcat
     1741 atagagttgc ttccgatatg cgaacatccg tttgacggct catggggcta tcaggtaacc
     1801 ggctattttg ccccgacctc tcgctatggc gagccgaagg acttcatgta ctttgtcgac
     1861 aagtgccaca gagagggaat cggcgttatt cttgactggg ttcccgctca tttcccgaag
     1921 gacgcacacg gcctgtttga atttgacgga cagtgctgct acgaatacga ggatatccgc
     1981 aaaggagagc atttgcaatg gggcacaaaa attttcgact acggccgtaa cgaggtcaga
     2041 agcttcctta cctcatcggc aatgttctgg cttgaaaaat atcacgttga cggacttcgc
     2101 gttgacgccg ttgcctcaat gctttatctt gactacggca gaaacgacgg cgaatggata
     2161 gcaaacaaaa acggcggcag agaaaacctt gaagcggttc agtttttgca gaatctcaat
     2221 gtccttattt tccgtgagca tcccgacgtc atgatgatcg ccgaggaaag cacctcatgg
     2281 ccactggttt caaagcctgt ctgcgacgga ggtctcggat ttaattttaa atggaacatg
     2341 ggttggatga atgactgtct gaaatatttt tcaatggacg gaatttacag aaaattcaat
     2401 cacaactgtc tgacgttctc atttttctac gctttttcgg agaattttgt tcttccaatt
     2461 tcacacgatg aggtcgttca cggaaaatgc tccatgatag gcaaaatgcc gggcgaatat
     2521 gatgaaaaat ttgcgggact gagagctttc tatgcataca tgaccgctca cccgggcaag
     2581 aaactgcttt tcatgggtca ggaatttgcc caattcattg agtggaatta taagcagggt
     2641 cttgactggc ttctgctcga ctatgaaaag cacgctcaaa tgcaaagcta tgtccgcact
     2701 cttaacacta tctacaaaaa gcagagtcct ctctggcaga tcgactatga ctgggagggc
     2761 ttcaactgga taagcaacga cgacattgac aattctgtta tttctttcag aagaatcgat
     2821 aagaataaaa aagaaattat tgcggtttgc aacttcacta atgttgagcg cagcggctac
     2881 cgaatcggcg taccgagagc aggaacttat aaagttattc tcaacacgga tgacgagagc
     2941 ttcggcggaa gcggcaaggg aaccgcagtt aaggttaaaa gtgaaaaagt tccgatgcac
     3001 ggctttaaaa acagcatagt gcttgacctt gccgggctga gcgttatcta tctgacgccg
     3061 ccggcacaga gaaaaaaaac aaaaaaaatc acgaccgata aggtcagcaa ataaaggagg
     3121 aataataatg tcccgaaaac atgagtgtat cgcaatgctt cttgcaggcg gacagggaag
     3181 ccgccttggt attctgacaa aaaacattgc aaaacctgcc gttccttacg gcggaaaata
     3241 ccgaattatc gatttccctc tttcaaactg cgtgaattcc ggcatctaca ccgtcggtgt
     3301 tctgactcag tatcagccgc ttgagttgaa cgattacatc ggcaacggac aggcttggga
     3361 tcttgaccgt gccgacggcg gcgttcacat tctctccccc tatcagcaga tatcgggtac
     3421 cgaatggtac aagggcacgg caaatgcaat ctatcagaac atcaacttta tagacagata
     3481 tgaccccgaa tatgttctcg ttctttcggg agaccatatc tacaaaatgg actattccaa
     3541 gatgctttct ttccacaagg agaagaacgc agactgcaca atagcaatgc ttgaagttcc
     3601 gtgggaggag gcttcacgct tcggtcttat gcttgtcaac gacgacggct cgattgccga
     3661 atttgaggag aagccaaaga acccccgttc aaacaaggct tcgatgggcg tttacgtttt
     3721 ctcatggaag aaactcagac aatacctgct tgacgacgag gctaatccga actcaagcaa
     3781 cgacttcggc aaggatctta ttcctgcaat gcacgccgcc catgagaggc ttttcgccta
     3841 tgaatttgac ggctactgga aggacgtcgg aactatcgac agcctttggg aggctaatct
     3901 tgacctgctc aatccgaagg ttgaccttga cctgacggac acaagctgga gaatttacgc
     3961 aaaagatccg atcacaggcc ctcactatgt ttccgacacc gcaaagatac agaattcaat
     4021 ggttgccgag ggatgcgaaa ttgagggcga ggttgatttc tccgtgctct ttgcaaacgt
     4081 aaccgtcgag gacggtgctg tagtcaagga ttcgatcgta atgcccggca gtgtaattaa
     4141 aaagggcgca gttgttcagt atgcaatcgt tgccgaaaac gttaccgttg aagaaaaagc
     4201 aagagtcggc aagcgacccg aggaaatgga aaatctcgac gattggggcg tttccgttgt
     4261 cggaatggga gtcaacatcg gcaagggcgc aacagttgag ccgaaagcaa tgatcggcga
     4321 agatgtaaag gaggcgtaag caatgagagc aaataatgta ctcggactta ttttttcaaa
     4381 tacatatgac aattatctca gcgagataac atcggttcgc tccatgggtt ccgtgcctta
     4441 cggctgccgc taccgcctta tcgactttca gctttccgca atggtaaaca gcggaatcgg
     4501 caaggtcggc atcattacaa agagcaatta tcagtcactc atggatcacg tcggacacgg
     4561 cagagcatgg gatctttccc gtaaaaacgg cggactttat cttctcccgc ccttcaatat
     4621 cggcacaacg ggaatttact cgaacaaaat tgaagctatt tccagcgcaa tgaatttcct
     4681 tcgcaactcg gaggaggaat atgtccttat gacggattgc aacgctatat ataatgttga
     4741 cttcacggat atgttcgagc gtcacgaaaa atcgggcgca gatatcacaa tcgcctacaa
     4801 acgttctccc gctccggcaa taagagagct tacaaaattt gagcttgcgg acgacggcag
     4861 aatcccttcg atcagccgcc gcagcgacga tgaggcaaac tattcgctca atgttatctt
     4921 tatgagaaag tcgcttcttg aaagacttat ttcggaagca atcggtctcg gctatcattc
     4981 gttcaccaag gacgttctcc agaagaacat taactcgctg aagatttacg gctgcgagtt
     5041 taacggctac gttaaggtta ttgacggaat tcagagctat tttgaagcaa acatggatct
     5101 tctcaagaaa gataatcgca aggcgctttt tgcaaaagaa aagcccgtat tcacaaaggt
     5161 caaggacgat gtaccggcta tctacggtct tgattccaac gcttccaatt cgctcattgc
     5221 ggacggctgc gtaattgagg gcaccgtcga aaacagcgtt cttttcagag gcgttcatat
     5281 cggcaagggt gccgtagtta aaaactgcgt actcatgcag aacacatata taagcgacga
     5341 cgttaaactc aactgcgtta tcgccgacaa aaatgttgtt atcaagccca acaaaaatct
     5401 ttcgggcgat gtcaagttcc ctgtatttat cggaaaggga atagtaatct aaaacatacc
     5461 gcaaaattac ggtgttgctt ttaagaaagg atgatttaga tgaaagtttt atatgccacc
     5521 tcagaggcgc tcccgttcat tgcgagcggc ggactcggtg atgttgcagg ctctctgccg
     5581 catgcactga gaaagaggct tatcggatgc cgtgtcgttc ttccgatgta tgacacgatc
     5641 aagcaagagc tcaaggacac aatgaagttt atcactcaca tttctgttcc cgttgcatgg
     5701 cgacgtcagt attgcggaat ttttgaagca aaatataacg gcgttatcta ttatcttatt
     5761 gacaaccagt attatttcaa gcgtgacaca atctacggac actatgacga tgcggaaaga
     5821 tttgctttct tttcccgtgc cgttcttgag ataatccctt atattgattt caagcccgat
     5881 attattcatt gcaacgactg gcagactgct cttactcccg tttactattc ggcaatgtat
     5941 gcaaactcac cgggctacga aaatataaaa acgattttca cgattcataa cattcagtat
     6001 cagggcgttt acggcaagga gcttcttgag gacgttctcg gaatccctgc cgacaaggct
     6061 tctttgcttg aatatgacgg ctgcatcaac ttcatgaagg gcgccatcga gtgcgccgac
     6121 aaggttacga ccgtcagccc gtcctatgcg gatgaaatcc ttgacccgtg gtattcacac
     6181 ggtcttgatt caattctcaa cgaacgccgc tacaagctca gaggtattct taacggaatt
     6241 gacactgtag gctatgaccc tgcaacagac aagtgcatta aggcaaactt ccccgccgac
     6301 gatatttcgg gcaaaaagat tgacaaggaa gagcttcaga aggaaatggg tcttcctgtc
     6361 aaacgcggcg ttcccgtaat cggaatggtt acaaggcttg tttcccataa gggtctcgac
     6421 ctcgtcaagg gaattcttga tgagcttctt gccacctcgg aaatgcagct tgttgttctc
     6481 ggctcgggtg actatgaata cgaaagctat ttccgctgga tcgcagaaaa atatccggac
     6541 aaggtaggtt taagaatcgg ctttgttccc gagcttgcaa gaaaaatcta tgcaggcgct
     6601 gatatgttcc ttatgccgtc caagagtgag ccttgcggtc tttcacagat ggttgcgcta
     6661 cgttacggta cggttcccat cgtccgtgaa acaggcggtc tcagagactc aatcaccgac
     6721 tgcggcgacg gtaagggcaa cggcttcaca ttcaagagct acaacgctca cgacatgctt
     6781 gacgctattc gacgcgcgct tactctctac tacaacggcg attggaacga gcttgttctt
     6841 cgtgctctca attgcgattt cagttggggt cgctcggcaa atgcatatat cagactttac
     6901 aaggaaactc tcaaaggctg agaaatcaaa aaataataat gtccccggtg tgtcctgcgc
     6961 cggggcattg taggtaaaag gaataatata tgaaatcata ctacattcaa ctcattcgca
     7021 acggtctgac cgagggcaat gtcagcggaa aatatgtcgg tcatattgat gagcctctca
     7081 gccgcgaggg catagctcag attgagcaga tgaaatccga ctacaaatat ccgcctgttg
     7141 acgctgtttt tgcaagtccg ctttcacgct gcactcaaac ggcgaaaatt atctatccga
     7201 attgtgaacc aatcgtaata aacggactga ttgaatataa cttcggcgaa tttgagggta
     7261 agacagcgga ggaattacag aatcaccctg tttttcccga ctggttggca ggcaaaaaag
     7321 gcgttgagcc tccgttcggc gagagtaacg acgaatttgc aaacagaatc gcaaccgcat
     7381 tcattaaaat cgttgacggc cttattgaaa ccggagtgac ccacacggca atcgtcactc
     7441 acggcggagt tattatggcg ctcctgaccc ttttcggact gccgcaggct cctatgcatg
     7501 agtggctgac cccaggcggc tgcggctata ccataagaat caccccctcg ctctggtcgc
     7561 agggtcgcaa ggttgaggtt attgcgcaga ttcccgaggt tgaaggtgaa aaagacagct
     7621 tcggcacgga tgacgacaat tttaatgttg aggactacat aaacgattaa ggatatttct
     7681 aaaaaaatag gagtgtttac atgaggcaat acagatcggt tataaaaaca aataaatttc
     7741 cacagcttac acgccgagtt tttacaacgg cagacgggct gaaatccgac aacgttacgg
     7801 cgatgcgttt cgacaataac ggcgtgcttt tcgtcggcac ggataagggt cttgcacgtt
     7861 tcgacggtga aaaattctca cccgtttcaa tcggggcaaa agatgcagcc atctcaatga
     7921 tttatttcaa tgccgaaaat catatgtttg tcggcgtagg caactcaatg cttgaatttg
     7981 acggcaaaaa gaaactttcg gagaaaaagt tcagctccaa gctcattgac attaaagttg
     8041 accttgacgg tgcaacgtgg attttgaccg agagcgtgct ctacaagctc ggtgaaaacg
     8101 gctatgatat tgaaatcggt gttcccggca agggaagctg cctcgccgtc tacaagaaca
     8161 ataaggtcta tgtaggcacg gcaggcggcg gacttcatgc tctcgtcggc aagcgctggc
     8221 actggtcgga gctcatggag ggcgttaccg gaattctcag caacacaatt acctgccttt
     8281 acatggatcc cgtcggcagc atttgggtcg gcacggacaa gggcgtttgt gtctatgacg
     8341 acaacagcta ttggctggat cattcaaaaa tcgacggtct gcccgatgct cacataacgg
     8401 gtatggcaat cgcagaaaac ggcgataaat atttctcgac gacaacgggt cttattcatc
     8461 agcacaacgg cagactttcg tattacggct ataagcgttg gcttccgagc ccgaacgcaa
     8521 caggcgttgc aatctctccc gacggctcgg tttgtgtttc gacagataag ggcataagcc
     8581 tttttgagac aaagatgata tcactcagag aaaaggcaaa cgagcttcgt gcacaaacgg
     8641 aaaaatataa cgttcgccat ggctatgtca ttgaccgtga cctcgagcat gagggagtta
     8701 tctcaaccga ggagggtcat attcttgctt cggacaatga aggcctttgg acaggtctct
     8761 acacggcggc tctttgcttc gaatatgcct gcacgaagga tcccgaggtt cgcaaggacg
     8821 ctcacagaag cttgcttgca atgataaagc tgaccgagat tacaggaatt gaaggcttct
     8881 tcgcccgtgc tatcagatat tccgacgccg ttgactacgg tacaggcgcc cgccatgaat
     8941 ggcacattac aaaggacgct gagggcagag aatgcgaatg gctcggcgag acctcgtcgg
     9001 acgaggttgt cggtcatttc tattgctatg caaactattt tgaccttgtt gcggatgatg
     9061 aagaaaagaa gctcattgcg gacacggtca aaaagattct cgaccatatt atcgacaaca
     9121 atttccgcct tgtcgattcc gacggtctgc cgacaacatg ggcaaactgg gatccggatc
     9181 ttctcaacaa tgaccacaag tggatatatg aaaagggtac gaactcgctc cagatgctta
     9241 ccttcctcaa aataggttat cacatcacag gtgatgaaaa atatgagaag cttttcattg
     9301 agtttgcagg caaaaaacac tttgcaatga atctcatgca gtataagatt cccgacggac
     9361 atctccttca tattgacgat aaccacgatt tccttatgat ttcactcttg atgaaatata
     9421 cggacgatcc gaacctccgt tcaatcttcg ccatgggtct tacccatcac tgggaggacg
     9481 aaaaggttga gagaaatgcc ttcttcaact ttgtttacgg cgcagtaacc ggcgaatggt
     9541 acaatgccga cgattgcatc gacgagctta tggatatgcc gatggatcag gttatgtggc
     9601 ctctttataa cagctaccgt aaggatttaa agtgggatat gtctccggca gagcttggca
     9661 tgcctcctca gctttatgag ccgttgccgg cacatgaaag aagaataaca agcaacgaca
     9721 gcannnnnnn nnntttcacc gttgacagcg gagctgagga cgttgcacag gaaatattca
     9781 aaaaatccga tgaaccgacc gcttacacaa tgttcccggg aacaggcgat gacaaaggtc
     9841 tcgttctcaa aacctgcatc aacttcactc acccgtactg gtatgcaaga tattacggtc
     9901 tcatagaaga ctgcgaataa ggagtgtgta aaatggggaa aaagattatc gttgccggag
     9961 gcggacacgg cggtattgcc gcagccgctc ttttggctga acacggcttc gacgtaactg
    10021 tttatgaaaa gaacaggcgt gaggatatgg gctacgactg gacggatatt tttgaccgca
    10081 agggtcttct tgcctgcgga atgactatgc cgcccgaaga taaataccgt ctaaaaaacg
    10141 atatgacctt tttcagtccg tcaagaaaca aaacacttaa acagcatgtt cccgaggatc
    10201 aacttgaaat ccagatggaa cgcaaggata tttacaacca cataatcgcc catgccgaaa
    10261 aatgcggcgt gaaattctcc tatgaaaccg aaattctcgc tcccgtaatg ctcggcggca
    10321 gagtcgccgg aataaaaacn nnnnnnnnna cgatttacgc agaccttgtc attgactcgg
    10381 caggcataaa ctcccccatt cgtaaaaatc ttcccgacgc cctcggaatc cagaaagagc
    10441 cggataaata cgatacattc tatgtttacc gtgcttttta taacaaaacc gctacggttg
    10501 aggacgacaa gtacagggtt tatcttctct ttgacaacaa gcagcagatt tgctgggttg
    10561 ccgacgacga ggaatacacc gacgttctta tcggccgttt ctctccgctg accgatgcgg
    10621 aggtggagga cacgctcgga aaactccgtg aacttaacga cgaccttggc acagaacgcc
    10681 tcagaggcgg acagtatgtc actatccccg ttcgtcagcc gctcggtctt ttggttgccg
    10741 acggctatgc cgcgatcggt gacagcgcat ttatgaccgt tccgattatc ggctcgggaa
    10801 ttgcaaacag tctcaaggcg gcaaggcttc ttgccgatgc cgtgatagcc gataaggacg
    10861 agcttttcag tgcggatact ctttggaaat atcagcgtga tttttataag aaactcggtg
    10921 caggccttgc tccgattgca attattaagc ttatggttgc ccgtttttct ccgaaggatc
    10981 ttgattattt ctttgataac gaaattctca attccaacga tatgactatc ggtgcagaca
    11041 ccaacagcat tatgtgcttt atcgaaaagc tcatgaattt caactcactc aagctcaagg
    11101 taaagggcgt tatgagcgat tttgttcttt caaaaaaggt tctcggcatg aatacaagaa
    11161 ttgccgccgt tatggcgata acaaaagcta tgcccgttcg atatgaccgc aagcgtgttc
    11221 tgtcatgggt cgagggatat aataaatgct ttaaaattga taaaggagat taaagaggtt
    11281 ttattcttct cggcagcctt cctgcccggg atttcacgct cttctctttt gtctcttata
    11341 gaaaacctga tttttaaaag attaaaaaaa gcagaagaaa aaaatgtacc tagtaaagat
    11401 acagcaaaag agagattgca tttagtttta atgcaagata gagcaaatgt ttctgctgac
    11461 tttctagaat taatgaaaca ggaaattata gaannnnnnn nnnaaaaagg aaagccacac
    11521 tgatgtgcgg ctttcctttt tggcgtcccc ggcgggattc gaacccacgg cctttcgctt
    11581 aggaggcgaa cgctctatcc tgctgagcta cggagacata ttcacttatt tatagcttaa
    11641 taagtttatc aaaaaagcgg cgaaatgtca aggggaaaaa ttccggtcgg aaaaattttc
    11701 atattcgttg aattcgccga taaataatgc ttgaaaaatt cggcacaata atataaaata
    11761 tataattggt taagaatgtt tcaataaaaa ccgaaaggaa gaagaattat gagcttactt
    11821 aacaagaaaa ccgtagacga tattaatgca aagggcaaga gagttctcgt gcgctgcgac
    11881 ttcaacgtcc ccctcaagga cggcgttatc accgatgaga acagaatcaa tgcggctctt
    11941 cccaccatac agaagctgat aaacgacggc gcaaaggtca tcctctgctc ccatctcggc
    12001 aagcccaaga acggccccga ggctaagttc tcccttgcac ccgttgcaaa gagactcagc
    12061 gagaagctcg gcaaggaagt cgtttttgcc gctgacgaca acgttgtcgg cgacaatgca
    12121 agggcagccg ttgccgccat gaaggacggc gacgtagtcc tgcttgagaa cacccgcttc
    12181 cgccccgaag agaccaagaa cggcgaagca ttctccaagg atctcgcaag cctttgcgac
    12241 atttatgtca acgacgcatt cggcgccgct cacagagctc actgctccac cgtcggcgtt
    12301 accgcattcg tcaaggaagc cgnnnnnnnn nnctcaagta tctcggcaac gctgttgaga
    12361 accccgcaag gcccttcgtc accatcctcg gcggcgcaaa gggtgcggac aagctcaatg
    12421 ttatcgacaa cctcctcaac aaggcggaca ccctcatcat cggcggcggc atggcttaca
    12481 ccttccttgc ggcaaagggc tacggcgtag gcagatcgct ccttgacgag agcaagatag
    12541 attactgcaa ggatatgctc aagaaggctg aggacaaggg cgttaagatc ctgctgccca
    12601 tcgacgtggt agccgctcct gacttcccga accccatcga cgcacctgtc gaggtcagca
    12661 cctttgacgc cgacgctctc cccgcagaca tggaaggcct cgacatcggc cccaagacct
    12721 cggagctttt cgcaaacgaa gttaaaaacg ctaagaccgt tgtttggaac gga
//
LOCUS       DAWBSW010000038        12719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1072_length_12719_cov_2.709097, whole genome shotgun sequence.
ACCESSION   DAWBSW010000038 DAWBSW010000000
VERSION     DAWBSW010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_12719_cov_2.709097"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3276..3285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9971..9980
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3147 a         3176 c         2703 g         3673 t
ORIGIN      
        1 ctcccgagcc gagggcaaac agcgattggt tcgtctgcca gcgggtatcc gtatcctcgt
       61 agtccttatg aataaacgaa tcaattcttt ccgcctgata cgggtcgagg attttatctc
      121 tgaacagaac aacaacggcg atcaaaacta tgcctgccgt tatgccgaga atgaaccatc
      181 ggttatcgac accgccgaga taaagcatac aaatgccgat aagcattata agaattgtac
      241 atgaaaggtg cttttcaagg tagataagca cacaaagaaa tccgagcagt ccgaaaagta
      301 caagagcgaa ccaccatttt gtttcgatct gatgtctgta tttttcaaga agccatgcga
      361 aggtcattat aataccgagc ttcgcaacgt cggagggctg gaatactatt ccgagcttca
      421 gccagcgtct gaattcctcc ttgccgtcga gacggtacgg atgaataaga acaagaataa
      481 gcaggataag cgatatcgcc gcaataacag gcgtggcttt ttttataagc tctggattga
      541 tttttgaaac agcaaacatg agcgcaacgc ccacaagagc gaatccgagc tgacgcttga
      601 tataatacgt tgcatcgtag cccgtcgcag cgtcgtattt tgcgctgata taactggcgg
      661 aaaacatcat cacaagtccg atagtcagaa gcatcagaac aagcaccata taaacgatat
      721 cgaatccgcc gccgatgaac acgtccaaac cgaacagtct gaacctgcct tttgattttt
      781 tttcggttga ggctgcggca tttctgcctg ccgtctgtct gagctgttcg gatgacattt
      841 tccgtatccc cctttgtttt gtttaggttt atttttgttt tctcttgaac caaagctccc
      901 ctgcatggag cgtcaaggat aggtttttgc tgatattttt acctcgaaaa tccgccgtaa
      961 tacatcaaaa gaacaccgat tattccgccg acaaggttga ctgtgcagaa caccgtgtca
     1021 atcttcttct cgctccagcc gcccatctca aaatgatggt gaatgggagc cattctgaat
     1081 attctcttgc cgtgagttat cttgaaatat cctatctgaa taacgtccga aaggccttcg
     1141 atgaaataaa taatacctgt caatataagg attatcgggc aatcaaaagc atatgcaatt
     1201 gcaacgacga gtccgccgag gaacattgag cctgtatcgc ccatgaacac ctttgccgga
     1261 tatctgttcc acacaaagaa gcctatgcag ccgccgagaa gagcggcgga aactatgccg
     1321 attccgaagg ccttgtgaac aacagccata atgataaagc tgattgccgc cgtagctgtg
     1381 actgacgagc aaagtccgtc aattccgtcg gtgaagttaa ccgcatttat tgcgccgtag
     1441 agtacgcaaa cgccgagaat ccagaaccaa atgccgagcg aaacattgcc gacaaacgga
     1501 atgaacatat acgggtcctt atccatcgcc agatagaggc ttgtaagata agcggcacag
     1561 attatgagct gaaaaatagt tttctggata atcgaaagac cgagattgcg tttttttact
     1621 acctttatgt agtcgtcggc aaaaccgact gcgccgaaca tcagcgccat aataagtcct
     1681 gcccaaagct tcgtcttttc gcttgagtca agtcccgctc tgctgactgt catcgaataa
     1741 caaacaagca caacggctgc ggagcaaaat attcctatta taaacatcag tccgcccatt
     1801 gtcggtgtgc cctgcttttt cttgtgccat ttcggcccga tatcgagaat tgtctgaccg
     1861 aatttgagct tatgaagcca tggaataatc acatagccga gcagtgcggt tattgcgaat
     1921 gcgccgaccg cgctgagtat aagtgcaaga tagctgttca tttgtttcac ccgtgtcctt
     1981 tcaacccttt gaattatttt attctgccgt aaaggtcgtt tataacatcc tcaagcgcca
     2041 tgccgcggct cgccttgaaa agaaccgcat cgccgggctt aagcgtttca aaaagcttgt
     2101 cggcaagcgc cttcttatcc gtgaagccat atacctcacg gacaccgcac tccctcgcct
     2161 tttctgccga ggcaagcgac ctttcgccgt aggtcataaa aacatcaacc tttgcttttg
     2221 ccgcgagcag acctgcattt ctgtgcgatt cctcacttat tgtgccaagc tccagcatat
     2281 cgccgagaac cgcaattttt ctttccgcct tcatctcaga gagcattttc aaagccgctc
     2341 tctgcgaatc ggggcttgca ttgtagcagt cctcaatgaa ctttattccg ttaaccgtct
     2401 tcatacgctg tctcatgccg gatggagtgt actttgcaag tcctttgaca gcctcctcgg
     2461 cggtgacgcc gagctgcaca cccgttccga aagcggcaag agcgttatat acattatgtc
     2521 tgccgatagt cggaataacg acatgctgat ttttgccgtc ataaactata tcaaattcag
     2581 tgctttcatc gcactgttca atgttaactg ctctgaaatc acaggtttta tcgtcgattc
     2641 cgtagtaaat cacaggatga ttctcaataa ccgcacccgc aagataaggg tcgtcaccgt
     2701 tgagaagaat aggacagttt tcacggagtc cgtcaagaat ttcaagcttc gccttgagga
     2761 tattctctct tgagccgagg ttctccatat gagaaacacc tatattgctg attattgcaa
     2821 ttgtcggctc ggcaatgaga gaaagctcgg agatctctcc cctgttgctc atgcccatct
     2881 cgataactgc tgcctgagtg tcctcatcca ttctaagaag cgttctcggc agaccgatgt
     2941 tattattcag gttgccctcg gtcttaagcg cattaaactt ctcattgagc acaaaccatg
     3001 tcatttcctt tgtggtcgtt ttgccgacgc tgcctgtcag accgacaacg gggatatcaa
     3061 atgtcgttct gtaccaatga gcgagcgcca taagagcctt gtgtgtatcc tcaactctga
     3121 gcatggggcc gtctgcgtca acatcgtgat gaacgattgc cgccgaagca ccgtttgcaa
     3181 aagcggaagc ggtgaaatcg tggccgtcaa agttttcgcc cttgattgcg atataaagac
     3241 aaccgggctt gcaaagtctt gagtctatac aaaccnnnnn nnnnncctct gtaaattccg
     3301 catcaatatt aaatgttccg ccgatgtgct cggcaatttc ttttaaagta agctttttca
     3361 tttttcaaaa ttcgctccgt tatcaagtat ttgcttcacg atttcacgtt cgtcatagtg
     3421 tatcttctcc gtcccgaaaa tctgatactt ttcatgtccc tttcccgcaa taagaaccgt
     3481 gtccccgggt tttgcttcaa cgagagcctc tcttattgcc tgtgttcggt cggtaatgcg
     3541 gacataggag tttttatcga gtccgcagac aatatcgtta attatcgaat cgggattctc
     3601 ggttctcgga ttatccgatg taacaaaaac cttatccgaa tatttgcagg caattttgcc
     3661 cattatcggt cttttggtct tgtctctgtc tccgccgcag ccgaatacgg ttatcagtct
     3721 tttgtcatat acttccctga ccgttttaag aacattttca agagaatccg gtgtatgtgc
     3781 gaaatcaata agtatattga ttttcctgtc attctttatc ttctcgattc ttcctctgac
     3841 cgttttcata tcggcgactg ccttgaccgc ccttttcatg ctgacgttca tcaccatgcc
     3901 gaccgtcaga gccgcaagag cattgtaaac cgagaatttg cctataacat tatatactat
     3961 atcatagctt ccgctttttg acgtaagctt atactcaacc ccgtcatggt gaagcctgac
     4021 tttttctgcc ataacatcag cgtctgtgtc aattgcatat gttacagttt tattccttgt
     4081 attctttttc ataaacggcg catattcatc gtccgcattt ataacgcaaa tatcactgct
     4141 ttgcatcaat atcgcctttg cttcggcgta gctttccata gatttatgat agtcgagatg
     4201 ctcacgtgaa agattcgtat aaactccgat atcaaaattt attccgtaaa gtcttttttg
     4261 aaccaaagcc tgtgacgagg cctccattat gcaaacatca cagcctgcca tcttcatcat
     4321 atataaatat ctgtgaagca ccgacggatc gggcgtcgta taatcggcag gatatttttc
     4381 gttgcctatt gcaactccga cagtgccgac aacgccgacc ttgtagccgt tctcttcaag
     4441 aatctttttt ataataaagg ctgttgttgt cttgccgttt gtgcccgtta cgcctatcat
     4501 tttgagcttt ctgtgacagt tgccgtaata attggcacac atgagtgcgt aagtctctct
     4561 cgtatcatta acgattatgc tgttttttat tcccatgtca cgctcacata ttacaactgc
     4621 ggctcctctt tcgacagccc tgcgtgcata gtcgtgtccg tcatttttgt ttccctttat
     4681 acaaacaaaa gcgcttgaat tccttacctt tcttgaatcg tcggtcacgt tagcgatttc
     4741 aggattaagg gtgtaaccgt tttttatttc aattccgtca agcaattcct gcagcttcat
     4801 agttacctcc gttcaaaaaa ctgacatgct gtattgcctt aatgatccga aactcccgat
     4861 gttgttctga aatataccgt tacggtagag ccgtattcta tttccgaatt tttcagcgag
     4921 ctttgacggt acgctgtcat actctcatcg gacagagaat ttccggatac tttaatattt
     4981 atacctgcat tgattgcggc ataattagct tccgttatcg tcattcccgt gaaatcggga
     5041 acaaccgttt tcagcttttt cttctgctcg tcggtataga gaacaacaac tccgccctgc
     5101 ggaatatact gtcccgaagc cggcatctgc gaaagaacct tgtcaccgtc accgacgaca
     5161 cgaacgctga acttattatc ctcaagagag gactgagcct cgccgacagc cttgcccgta
     5221 acctggacgt caagcaaagc cttctcggaa tcgttgtact gacgctcaat attgagataa
     5281 gtgagcgtat tttcgataac ctcgcctgca accggagccg caaccgctcc gccgcccgtt
     5341 gctccctcag gctcatctac aacgataagc acggtaacct gaggatcgtt tgacggtgct
     5401 actccgacaa aggacgccgc aacctcgccc ggtttgttca gcttgtcgga agtacctgtc
     5461 ttgccgccga ctctgtagcc cgcaacatag gcatttctcg ccgttccgtt gagagcgacc
     5521 tgctccattg aagccatgat tttttccgat gttgattcgg aaatgacctg tctcttttca
     5581 atcggctgtg tctgagaaac aacattgccc tcgctgtcaa gagtcttgct gacaacatac
     5641 ggctgcataa gcttgccgtc gtttgcgatg gcattgactg ccgttatcat ctgtatcggc
     5701 gtgatctgga aggactgacc gaacgatgag ctggcaaggt ttacctttgt cattttgtcc
     5761 ttttcaaagt aggttacgcc tgccttcggt gcggtttcac ccggaaggtc gatacctgtt
     5821 ttttcggtaa atccgaaggc ttcaaaatat ttatagaagg tatcaacgcc gagtcgctga
     5881 ccgatggtga tgaaataggg gttacaggaa ttcataaggc cctcggtaag agtctgggtt
     5941 ccgtgacctg ccgtgttatg gcatgaatag attctgtctg caacggatat tgagcctgtg
     6001 caagtgaact tttcgtcata tttaacggcg ttctcctcaa gtccggcagc catggttatt
     6061 accttgaaaa ccgagccggg ctcatatgta tccgtaagcg ctctgttgtt ccactgagcg
     6121 tacattgcgt ttttctccgc ctcgtccttt tctgcggtat cggtaatttt attgagcgac
     6181 tcaaccgttc ttttgtcata aaccgtgtac gggtcgttcg ggtcatagtc gcccttggag
     6241 gacattgcaa gaatagcacc ggttttaacg tccataacaa tgcccgtaca gctcgccgcc
     6301 ttgcttgttt catagcagga gaaaagcgca ttttcgagat atctttgtat cgtttcgtct
     6361 attgtcagga caaggctcgt tccctgcttt gcctcataaa cggacttata ctcaatatcc
     6421 tgtgcaacgg aggaattgct ccttgccgaa atcactctgc cgtcaacgcc cgtcagtgtg
     6481 ctgttgtagt attcctcgat tccggaaaga ccgttattat ccgttcccgt gaagccgaga
     6541 atttgcgagg caagcgtcga atagggatag taacgcttaa cgtcctcgtc aatcataacg
     6601 atagagccga agctttgctc ctgattgtcc ttatcaagga aagtgccctt tataaaggct
     6661 tccagcttat cctttgcttc tttttccacc ttggttttga tgcttacgct gttgtattgc
     6721 ttatatgatg ccttatcttc aagatacttt ctcgaaacgc cgagaacgct cgaaagggtc
     6781 gaatacacct tctcctgagc ctgagccgga atatcggcaa aagcggtcgc gttgagagaa
     6841 atcttccaag ccgaggcact ctgggcaaga accgacatat tagcgtcata aatgatgccc
     6901 ctttgtgcgg ttatcgccgt atcgctgagc tgctgagtct ccgcctttgt tttgaagaaa
     6961 tctcccttgt aaagctgagt atacgccatg ccgaatattg tggcaccgaa tccgagaata
     7021 atgattatcg caagcaggcg cattgttctc ttttgaagat ttttcattat tattttttta
     7081 ggtctgagaa aaccgtttgt gcctctttgc aaccctatca tcctttcgcc cttcattttt
     7141 gcgctgtacc cttaaaatca ctctaagggc ggataaaaaa tccgccctca ccgttcatat
     7201 tataaatatg tctgatatca tttttatatt cattgattcg tatccttctg agctgacttt
     7261 gtgttgttat catcctctga catatcaaaa tatcttacct gatagctttc acgcttaacc
     7321 atgccgagct ttgtctgagc atattcctca attttatcca tcgacataag agagttgaac
     7381 tgcatctgca agcgtacatt ttcgctctgt gcggttttga gagccgttct ctcgtcggca
     7441 agctcgctct gataatttgt cagcattact ctgctgtaga taagcgagcc gagtataagc
     7501 aatgcggcaa tggcaataat tcctgctcta agtgcagcga tcctgctggc tcttacctct
     7561 gtctttgcct gttcaaagct gatcggcttc ggattgatta ctgtcgggat ttcaattttt
     7621 ttctttttgc gttcaacggg aacgtatccg ttttttgtat tatcgaacaa gtcgagatca
     7681 agggccaagc cgtcattacg attagccata tccgtcagct cctttcaaaa tttaacgtca
     7741 ttttaattta atctttctta tttttgtttt atatcttttc tattgctctg agccttgcac
     7801 tgtgtgctct gaaattctcc ccaacctcgt tctccgtcgg tgccttcgac ttgaacggga
     7861 gcttacctct cggctttctg ccgcagacgc aaacgggaaa ttcaggcgga caaatgcagc
     7921 cctcacagaa tttattgaac ttctttttaa ctattctgtc ctcaagcgaa tggaatgtga
     7981 ttatcgctat cctgcccgag gggttgagtg cctcaaacat atcgtcaagc gcacgttcaa
     8041 gcttttcaag ttcgccgttt acctcgattc tcagcgcctg aaacgtcttt cgtgccgggt
     8101 gtccgtcacg cttggctttc ataggcatcg aagccttaat gatatcaaca agctcaaagg
     8161 tcgtttcaat cggcttcttc tccctcgcct tgacgatatt ccttgcaatc gacggagcaa
     8221 atttttcctc accgtaggtg taaatgattt tttgcaattc gctttgtgag gcggtgttta
     8281 caagatctgc ggcgctcatg ccctccatgc tcattctcat gtccagcggt gcatccttgt
     8341 gaaatgagaa tccgcgttcg tcggtatcaa gctgatgcga gctgactccg agatccgcca
     8401 taattccgtc cacgccgtct attccgagat tctcgagtat cgacgatata ttaacgaaat
     8461 tgtcgtgaac aatcgtcact ctgccgttat ttttaaaacg ctcctgcaaa ttcgcgacag
     8521 cttcggggtc ttggtcaatc gcaatgatcc tgcccgtcgt cagtctttcg gcaactgcgg
     8581 cgcaatgtcc tccgccgcct gcggtgcaat caacgtaaat cccgtccggg cgaacattga
     8641 gagcgtctat cgtttcattg aataatacgg ggatatgctt gaattcgctc atcagaacgg
     8701 aacaacgaag tcggcaggct cgtcatcctc tctcatcatt tcgtcgaagc gatccttcgc
     8761 ccaaatctcg gctctctgac cgacaccgat gataacgata tcacggacaa tgcctgcttt
     8821 ttcgataaac cttgccggca gaacaacgcg gctctgcgaa tccatctcgc aattctcgct
     8881 gttgagaaaa atcgttctct gaagcttagt cctgtcaacc ccgtcattga cgaacaaaag
     8941 ctcgttggta atttcctccc actgctcctc tctgtaaatt ctgaggaact gctcgccggg
     9001 cttggcaaag acaacaaact ccttgctgcc gatcatatct ctgattttcg gaggcataac
     9061 tattcttttt tttgcgtcaa gagtacggtc ataatagccg ttgaactgca taaaagctgc
     9121 ctccttttcg aaaaactctt ccacttcgtg ggttttatgt gtaaattcac tccacttttg
     9181 taactttatt atacaataca atttccaaat gtcaagaaaa aatattgcac aaaaagctta
     9241 aataatatta caaattgtta caaagccgga aataactctt ttctcaatca aaaagtattg
     9301 acaacaaaca aaaatatgtt taaaattcac ttgtgacttt tcgccgcaaa taacacacat
     9361 tataattaag gaatgattaa atgaagcttt ttgtaaatcc cgataaaata atcggcaaaa
     9421 taaagcctat gaacgccgtc aacaacggcc ctgtgtacac cgaaaatgcg gatcagaatc
     9481 tgaccaatct gccctatttt aaggaagcta acatacccta tgccagaact cacgattcct
     9541 ctatctgcta cgactacggc ggggagcaca cggttgacat aataaacata ttcacggact
     9601 ttgatgcaga tccttactcc cccgagtcct acgatttcac gctgacggat atgtatcttg
     9661 acaatatcgc gcgctcgggc gcacgggtgt tttaccgtct cggcaacaag atcgagcatt
     9721 ggccgaagca ttacggaatc atgccgccga aggatttcaa aaaatgggcg gtcatctgtg
     9781 agcatattat ccgccacatg aacgagggct gggcaaacgg tcatcacaga ggcattgttt
     9841 actgggaaat ctggaacgag cctgatttca acaataaatg ctggacgggc actcccgagg
     9901 agtttttcga cctctttgaa atcacggcga aacatcttaa atcctgcttt cccgagatca
     9961 aaatcggcgg nnnnnnnnnn gccgtgtgtc gctacaatgc agactggctg attcctttct
    10021 ttgaagagat gaagaagcgt aatgtgccga tggatttcta ctcgtggcat tgctactctt
    10081 caaaggttga ggattttata agagataccc gtctgcacag agccttgctc gaccgatacg
    10141 gctacagtga aagcgagagc atactcaacg aatggaacta tgtctgcggt tggagcggcg
    10201 aaggatggaa aaaaagcctg gcaaccgagc tttcaataaa aggagcggct tttatcgccg
    10261 ccgttgcaac agcctgccag catgaaaagc ttgatatgct gatgtattac gatgcgagga
    10321 tcaactcaac aatgaacggc cttttcagac ccggcacatt ggaagtgcgc aagggttact
    10381 atccgctgaa aatctgggga gaaatgcttt caagcgacgg cgaatgtgaa acatcatgtg
    10441 acattcccga catctatgcc gtatccgccg ttaaagacgg tgcaagcgtc acgatgataa
    10501 cctattactc ggatgacgac gaagccttgc ctcgcacatt caccgttgag ctttcgggtg
    10561 acgttcttcg cacggtgtat ttgctcgaca gcactcacga tatgacagct tgtgaaacaa
    10621 ttgctccgga ttgcggctca ttcagattga ccatgcagcc gaacacggtg gtggtaatca
    10681 gataaatgaa agactatatt tttcttataa taattctcct tttgagagtt cctcagaatt
    10741 tttcaagcaa aaagacaagc ggtcttgtta caaactcgca atcatacttt ctgtacggaa
    10801 catacagtta cactttggca gggctaattg cctttgtaat gcttctgttt gacggaatgt
    10861 cgggcttcag tcttcctgcc gtcggaattt ccgctcttgg tgccgtttcg ctcgccgcca
    10921 gcctgttctg cagcatcgag gcactcaaaa acggcgtaat ggttctcgcc gcaatggccg
    10981 gctcggcagg acttcttctt ccgtgtattg caggaatttt catgtttaac gagcctatga
    11041 agcctatgca gtttatcgga atcgcactgc tcattttttc cggatggctt ttaatcggtt
    11101 attcaaaaga gcagacaggc agctttacgc cgagaacact gcttcttcta atcggaagta
    11161 tgctttccaa cggttcggtc atgctcgctc aaaagatgtt ttcgaaatat ctgcccgaca
    11221 ccagtgtttc aatcttctcg tttttgacat tcggtcttat cggaatagga atgtttatcg
    11281 gtcttgttcc gtcgcttctc agtcagagcg gcagagccaa aattgcggca gttccaaagc
    11341 ctgttttttt gtacggtaca atttcgtcaa tcattctgct taccatcaat cagcttgcaa
    11401 cgcttgaggg acgcaatgtt ccctcggcaa tcatgttccc gataaacgac gccggagcaa
    11461 cgataataac ggcgattacg gcggcaattt tcttcaaaga aaagctcacc gttcgcagtg
    11521 tttgcggact gattctcgga ataggttcgc tcatagtaat caatcttttc ggataaaaaa
    11581 gcacacctca aaagcatcat acttttgggg tgtgtttcga ttttatacaa tttataatac
    11641 ttttttaaga tattgacccg tatatgagcc ttcgacagcc gcaacctctt cgggagttcc
    11701 tacggcaata atattgccgc ccttgtcgcc accgtcgggg ccgagatcaa taacatgatc
    11761 cgccgtttta ataatatcaa gattatgctc tattacaagt accgtattgc ccttgtcaac
    11821 aagcttctgc aaaacctcaa taagcctgtg aacatcggcg gtgtgaagtc ccgtcgtcgg
    11881 ctcgtcaagg atataaatcg tcctgcctgt tgcaggcttt gcaagctcgg ttgcaagctt
    11941 aactcgctgt gcctcgccgc ccgaaagcgt cgtcgcagac tgtccgagct tcacatatcc
    12001 gagaccgacc tcgaacaagg tcaaaagctt tttatgaatt ttcggcactg cggagaaaaa
    12061 gttcaccgcc tcctcaaccg tcatttcaag cacatcataa atattcttac ccttatactt
    12121 gacctcaagc gtttcacggt tatatcttgc tccatggcaa acctcgcacg gaacaaaaat
    12181 atcgggcaga aagtgcattt caatcttcat gattccgtcg ccctgacagg tctcacagcg
    12241 tccgcccttg acgttgaacg aaaatctgct tgccgtgtat cccctgctct ttgcgtcaga
    12301 ggtcattgcg aaaagctcgc ggatatcgtt aaaaacgcct gtgtatgtcg ccggattcga
    12361 gcgtggcgtt ctgcctatcg gcgattggtc aatgtcaatg accttgtcca ggttttcaat
    12421 gccgagcatt tccttatgct tgccggggaa gcgctttgcg ccgttcagct cctcggcaag
    12481 acgtttatag ataatttcgt ttacaagcga ggacttgccc gagcccgaag cgcccgttac
    12541 gcaaacaaat tcgccgagcg gaattttaac atttatatta ttaagattat tttctgcggc
    12601 gcccctgact tcaaggagct ttccgttacc ctttcgtctt ttctgcggca cgggaatttt
    12661 cttccttccg ctgagatact gacccgttat cgacttctca cagttcatta tatcctgcg
//
LOCUS       DAWBSW010000039        12367 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1114_length_12367_cov_3.076511, whole genome shotgun sequence.
ACCESSION   DAWBSW010000039 DAWBSW010000000
VERSION     DAWBSW010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_12367_cov_3.076511"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1417..1426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2000..2009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3412 a         2814 c         2726 g         3395 t
ORIGIN      
        1 tgcagacccc gaggagggcg gctacggcgg ctggggaatt tatcttgatg agggctcgtc
       61 gtatatgctt gttgaaaaaa atcttgttta ctcctgcggc agtgactctt atcatctcca
      121 ttacggctca tacaatactt taaggaacaa tatttttgcc ctcagcggag aatcccaact
      181 tagaatcgtt accgcaccga ctcgttgcac acccgatgac ggcggacaaa aaaccgttga
      241 cctttacaac aatattattc tcacggatca aaagacctgt gtcttatcct atatgcataa
      301 cacttccaca atggacgaac gcaacaatac atattgggat ctttcgtttg gaggaaaact
      361 ttatgcaagc aaagaaacaa atataaatcg ttcattaagt attaaaacgg caatcagaaa
      421 aggatttata cattcgccca taatagccga cccaatgttc agagatgccg caagctttga
      481 ttttgaactc agtcccgatt ctccggcgat cgccgcaggc ttcgagcctt gggattactc
      541 aaacgcaggc actctttcgg aaacaacgat aggtctttcg accgagggcg gcaacaccgc
      601 ctacaatgca aattcggcgc acgttccgat gactcctgca aaggagcaaa aacgcttcct
      661 tgccgaaata atcaacgcaa tttatttctt ctttaaaaac cttttcggct cgatttgctg
      721 agataagttt attaaaatcc gcaagaactt attttgttcc tgcggatttt ttctcaaatg
      781 atttattaaa gaaactataa acctcaattc aaatattttc aattcaatat ttgcagaata
      841 tttttatagc cccttgacat atgcctatga gaagaatata cttatcttag ttagcatatg
      901 ctaaccaaag ggggctatgt tatgaaaaag aatgttttca gagtaattct tgcagttgtt
      961 cttgttgcaa cggttgtagt tttcaccgtg agtgtaggcg cagttacagc cgctcactat
     1021 aaaagaggaa catatgaggg cagcggccga ggaaagttta gtgacgttgt cctctcggtc
     1081 aaaataaggc gtggcaaaat cacagctatt gacgttatat cacagaacga aacaccgaat
     1141 tactggaaac gggcagtcgc agtgattgac agaataattg aatcaaacaa caccgccgtg
     1201 gacggcgtta caggggcaac caaaagcagt aacgcaatca aagccgccgt tgacaacgcc
     1261 ttgaaacagg caaccgtcta tggggacgtt tcatttttaa caccggaatt tttaaagaat
     1321 ataacggata tctttattcg ggcgctttcg tttataaaag tcgcttgctc ctgataatat
     1381 aaaaactgtc gcaatggtaa ttcactgcga cagtaannnn nnnnnnaatt ttttatgcat
     1441 cgagcttagc tcgctttttg tgttcaacca aaggtgctgc gttgttttcc acgcaatcct
     1501 cggtcacctg aacgctgacg attgatttat cgctcggaac agcatacata atcggcatga
     1561 gaaggttctc cataatggag cgcaatcctc ttgcgccggt tttcttttca agggtttttc
     1621 tcgctatcgc cgtgagtgcc tcagaagtaa agtcaagctc aactccatcc atcgaaagca
     1681 gagatttata ctgcttaaca atggcgtttt tcggttcggt cagaattctt acaagagcgt
     1741 tttcatcaag ctccttgagc gttgcgataa cggggagtct gccgacgagc tcgggaatta
     1801 tgccgaactt tgtaatatct tgctgaacga cctttgccat cgcattttca accgtaaagg
     1861 cttccttatc tttgatgtcg gcttcaaaac cgagagccga accgccgata cgtttttcga
     1921 caatcttttc tattccgtca aatgcgccgc cgcagatgaa aagaatattt gaggtgtcaa
     1981 cctgtatgca ctcctgctgn nnnnnnnnng gtacattcgc aactgttcct tcaacgattt
     2041 tcagcaatgc ctgctgaaca ccctcgccgc tgacatcacg ggtaattgac ggattctcgg
     2101 attttcttgc aatcttgtct atttcatcga catagattat tccgcgctcc gccttttcaa
     2161 tatcgaaatc ggcagcctga attagcctga gaagaatgtt ttcaacatct tcgccgacat
     2221 agcctgcctc agtgagcgtt gttgcgtccg caatcgcaaa cggaacgtcg attatcttgg
     2281 cgagcgtctg agcaagcatg gttttgccta cgcccgtagg tccgagaaga agaatattac
     2341 tcttctgaat ctcaacgtcg gaatcctcct gcttgaaaac acgcttatag tggttataaa
     2401 cggcaacgct gagagtgacc ttggcttcat cctgaccgat aacatactcg tcaagacctt
     2461 ttttgatctc catcggagtc ggcagcttga agtcctttgc ttttctcttt cttgctctgc
     2521 ctgccgccgc ctgctcctct gccataattg cactgcaaag gtcaatgcac tcgttgcaga
     2581 tataaactcc cgggccggcg attatttttt caacctgtcc gtcggtcttg ccgcaaaagg
     2641 aacagcgcac acttttttca cgtctttcat catctcttgg catcgttaca cccctgattt
     2701 atcttttgta aagaacctta tcaacaagac cgtaatccat ggcctcctga gctgtaaggt
     2761 aattgtcacg ctcggtgtca atagcaatct gctcaatagg cttacctgtg ttctcggcaa
     2821 gaatctgatt gagcttttct cttgtcttaa gtatttggtc ggcaactatc ttgatttctg
     2881 ttgcctgacc ctgtgctccg ccgagcggct gatggatcat gatagtgctg ttcgggagcg
     2941 caattctctt gccctttgcg cccgaggaga gcaaaaatgc gcccatcgaa gcagccatac
     3001 ccatgcaaat cgtcgaaacg tcgcacttga tatactgcat cgtgtcataa attgcaaagc
     3061 ctgcggtaac agagccgccg gggctgttga tgtaaaggct gatatcctta tcgggatcct
     3121 gtccttcaag gaaaagcatc tgagcaacaa caaggcttgc ggttgcgtcg ttcacttcat
     3181 cggacagaac aatgattctg tcgctgagaa gacgagaaaa aatatcgtaa gagcgctcgc
     3241 ctctgttggt ctgttcgatg acataaggta ccagtgccat atttattcct ccattcgagt
     3301 tgtttcgttg tttttttcag cgtcataata gtgcaaatga atggcaaaga gccatccatc
     3361 cgcattaatt ataatcattt gaaagtgaaa ttattcagcg tctgccggct tttcttcctc
     3421 tttctttgct gcagccttct ttgttgtctt cttcggcttt tccgctacga tagctttagc
     3481 gtttgcaaca aggaaatcaa ctgtcttttt gccgacaacg tccttcttaa cgtcctcaat
     3541 attaacccac ttcttgacct gctcaacgtc gagctgatac atatcagcca tttccttgag
     3601 ctgagcctcg gcctcctcgt cggttgcctc aatcttttca agctcggcaa ttttttcaac
     3661 tgcaagctgg agcttaacct gcttaacagc gttgtccctc atgctctcct tgaacttgtc
     3721 tctgtccata cccatgtact ggagataagt atcaagagga atgccctgct gtgcaagtct
     3781 gttctcgtag tccttaacgt cgtcctcaag cttattgtca tacataacct cgggaatctc
     3841 gccctcaacg agatcaacga ctttttcaag gactgcgctc tcgaagtcct tctcaacatc
     3901 ttccttcttc ttatcggaaa gctctgcctt gatcttcttc ttaagctcgg cgagtgtatc
     3961 aacctcatcg tcaacgtcct ttgcgaaatc gtcgtcgaga gtcggaagct ccttcatctt
     4021 gatttcgtga agaactacct tgaatacggc ttccttacct gcaagctcgg gagtatactc
     4081 ggtcgggaaa gtaacattta cgtcgaactc gtcgccggtc ttgtggccga tgatctgctc
     4141 ctcgaagccc gggataaact gacccgaacc gagggtaagc tcatagttct cgcccttgcc
     4201 gccgtcgaat gctacgccgt caacaaagcc ctcaaagtca ataactgcaa tatcgttctt
     4261 cttagccttt ctgtcgtcaa cagtaacaac acgagcgttt ctctcctgca tggaagcaag
     4321 ctctttctta acgtcgtctg cgcaaacgga agcgtcgccc ttgtctgcct cgattccctt
     4381 ataattgtca atcgagattt ccggcttaac ggtaaccttc atcgtcattt caacgccgtc
     4441 cttgccgatc tttgtaacgt caagatcgtg aggagctgca acaggctcaa ttccggcctc
     4501 tttgatggca tcgccgacaa cgccgggata tacgatatca agcgcatctt catagaatac
     4561 gccctcgccg tagaactttt caatcatttt aagcggagct ttacccttac ggaaaccggg
     4621 aagctgaatc ttgttcttct gcttgttgta agcctgaaga attgccttgt tgaagtcctc
     4681 gccacttacg ctgacttcaa gagtgtagac gtttgtttct gttttgttag ctgattttaa
     4741 cattaaaaac ttcctcctgt gtggtatttg gagcgtacat acttccacaa atacctatta
     4801 acttttgtat tatagcagat gttttaatct ttttccatac cttttttaaa aaattttatt
     4861 atttacagaa ttattttcaa gttttttaca ttacattatg agcaaaaggt cgaaaaaggt
     4921 tgtctgtaaa ggtgataagc tttacagaca accttttgat tatatgttct tttctgtcaa
     4981 caaaatttta taaaaattat gccaaaacca ttgacaaatc aattcttttg tactacaatg
     5041 cagtaaaccg atgaggaaga gtgagtagat tccgacggac acttcagaga gctgtaggtt
     5101 ggtgcgatac agtgtggaaa tcagagttga atggacttcc gagggcgagc agaaataaaa
     5161 agtatgcgag ccggagagag aactccgtta acaataaccg tcgtataatg gggtacgagc
     5221 taagtggaca cttgaatgtg tcaagcaggg tggcaccgca ggagtatatc tcttgtcccg
     5281 gcaatgttgt cgggacagga gttttttgtt tttatgcccc gacacggctc agacaaaaat
     5341 attttttgaa aaggagagtc agttatgagc aacacagaaa aaattccgta caaaatctac
     5401 ctcgatgaaa aggaaatgcc gaagcagtgg tataacgttc gtgcagacat gaagaacaag
     5461 ccggcaccgc ttctcaatcc cggcactcat cagccgatgg gctttgacga tcttcgtccc
     5521 gttttctgcg acgagctcat taagcaggag cttgacaatg acacggctta tttcgacatt
     5581 cccgatgaga ttcttgattt ttacaaaatg taccgtccgt ctcctctggt aagagcctat
     5641 tgccttgaga aaaagcttgg tactcctgca aagatttact ataaatttga gggcaacaac
     5701 accagcggaa gccataagct caacagtgcg attgcccagg catattacgc aaagaagcag
     5761 ggtcttaaag gcgttacgac agagacaggt gcaggtcagt ggggcacggc tctcagcatg
     5821 gcttgtgcat atctcggact tgactgtaag gtatacatgg ttaaatgctc ctatgagcag
     5881 aagccgtttc gccgcgaggt tatgagaacc tacggagcat cggtcactcc cagcccgagc
     5941 acggaaacag aagtcggcaa gaagattctt gcagagcacc cgggcacaac gggaagcctt
     6001 ggctgtgcta tcagtgaggc cgttgaggtt gcaacaactc atgagggcta tcgctatgtt
     6061 ctcggcagcg ttctcaacca ggttcttctt catcagtctg tcatcggact tgagtccaag
     6121 atcgcaatgg acaaatacgg catcaagccc gatatcataa tcggctgcgc aggcggcggt
     6181 tcaaacctcg gcggacttat ctctccgttc atgggcgaaa agctcagagg ggaggccgat
     6241 tatcggttca tcgcagttga accggcaagc tgtccgagcc tcacaagagg taagtttgct
     6301 tacgatttct gcgacaccgg catggtttgt cctcttgcaa agatgtacac actcggcagc
     6361 ggtttcatcc cgtcggcaaa ccacgcaggc ggtctgcgtt accacggcat gagctcgatt
     6421 ctcagccagc tttaccatga cggtcttatg gaagcgacaa gcgttgaaca gaccaaggtg
     6481 tttgaagcgg cggagcagtt cgcaagagtt gagggtatcc tccccgctcc cgaaagcagc
     6541 cacgcaatca gagttgccat tgatgaggct ctcaagtgca aggaaacggg cgaagaaaag
     6601 acaattctct tcggcttgac gggtacgggc tacttcgata tgtacgcata cgaagcattc
     6661 aacgacggca caatgaccga ttacgtcccg accgatgccg aacttgaaaa gggctttgca
     6721 ggtctgccga aaatggactg attttcttgc gtgcaagcct tgacaaacca atataattga
     6781 tgtaatatat tcacagcagc aggggtgctg agaaatcggc tgagatcata cccttaacct
     6841 gattcggata ataccgacgt aggaagctat tgaagtaaaa tctacgcccg gtggttaatc
     6901 cgccgggcga ttttctttga tcaggtaatg ctgtgaaagg agttttatta tgaaaagcaa
     6961 aacaatggac aaatcaaacg aaacgacctt aaaattgacg gtcagcgcaa tcatggttgc
     7021 actttcaacg gtgctttcat ttgtaaagtt tagcgagctt ccgtatggcg gttccgtaac
     7081 gctcttcagc tttgtgccga ttcttttcgt aggctatgct tacggtgcaa agtggggtct
     7141 cggtgcaggt cttgttcacg gaatccttca gatgattttc ggaatctccg gtgcgactgc
     7201 aggtgcaggc ttcaaatggt ggcaggttgt tctctgcgca cttcttgact atcttgtagc
     7261 attcagcgta ctcggtctca gcggtgtatt caagaagtca atcaagaatc cgcaggcatc
     7321 atttgcactc ggcgcacttt tcgcctgcgt actcaagtat atttcgcact tcttctcagg
     7381 cgacattctc tttggcggct gggctgaatg gtttttcaca gagggcggcg gaatgtccta
     7441 cggttcaaag attctttcgg catacagcgg aaacgttctc agcgcagttt attcggttat
     7501 ttacaacgca acgttcagca ttcccgaaac cgttctttct cttgtaatgg ttgttgttct
     7561 catcagcatc aagccgattc gcaaggcagc gggaatcaaa taatcaaaca tatagccaaa
     7621 cacggagctg ttgcatttgc atagccccgt gttttttttt cttatcataa acaaatactt
     7681 tttcgctttc ggtatcaatg aaaccagttt tataaaaatt atgcttccga tgaatgaaac
     7741 aacctcaagg taaatcaaaa tcatcggctg cgatattttg gttgcaagaa aagaatacgt
     7801 cgttgctcgg agaatttcca taattattat atgtacaaaa taaatgccga acgatatttt
     7861 tgagaggccc tcaacagccg gtctgattcc ggcgatacga tccgagtatc ttctgaatat
     7921 ttcaaataga aaaactgcac aaacgaaaaa tccgacaaat tcatatctca gaattacatc
     7981 cggcttttgc gagaacaaaa acatctgtat cccgacagaa acagcaaaag aaagaacagc
     8041 accgagcatc aataaatatt tattaaggtt tttcaatgcg cccttgctga ccagatatcc
     8101 gacaaccaca atcaataaat attgcggtaa aatcacatct tcctgaacag aaaaggtcga
     8161 tgaacctaat ccgcgaagaa tatcatttaa ggtggggata agatagtaat aaatcagggt
     8221 tgccgccatt gggatcataa ttatcatctt tggaacctta ttttttatca ttatgaggaa
     8281 cggcaggaaa agataaatgc ataaaatcat cgggatatac cacatgctgt ggaaagtagt
     8341 ttgattgaca aaaaacatag tttcaagcaa tccgagaatt gacataaata tactttcggt
     8401 gcggaaatgt ccttccatga tataaattac ccagtacata ataaacatcc aaatctcgga
     8461 agtaataagc agactcagca aattatgctt gtagaacttt ttaatatctt cggcattatt
     8521 gatttcttta ttaaaaagca atgcgccgga aatcataaga aacagcggaa cacctatgcg
     8581 actgaaaacg taaacaaaag ttttaaataa agttgaccct aacggaatgc tgaaaaattc
     8641 cgctgattga ccctcataga catggtatgc tcgattgact gcatgattta acgttataga
     8701 aattatcgca acgcatcttg ccacatcaag ccagaaaatt cgagaatcgg aaatcttttt
     8761 attcatatat ttatcccctt tgcttaaata tacgttattt ctttccttta accttatccc
     8821 aatatgcctt tgctgcctgc tccatcttgt tgtcgccgaa ttcataatac ttggcatttt
     8881 ttatggcatc cggcagatac tgctgaggaa tccaatgatt ttcatatgta tgagggtaga
     8941 gatagaattg acccttattc gggttgtcct cgccgtcaca gtgtttgttc tgaagccggc
     9001 ggggaatggg accgcttttg ccctttttaa tatcactcag agcggcatca attcccaggt
     9061 atgccgaatt ggatttcgga gaggtgcaga caagaactac cgcatcggca agcggaattc
     9121 ttgcttccgg caagcctacc atgagcgcag catcaacggc agcttttaca atcggaatta
     9181 tcatcggata agcaagtccg acatcttcac aggcgcaaac cataagcctg cggcaagccg
     9241 agggcaggtc gcctgcatca agcagccttc cgagataatg caaggctgca tcgggatctg
     9301 agcctcgcat ggatttctga taagcggaaa caatatcgta gtgctcgtca ccgtcacggt
     9361 catacttcat tgcactgcgc tgagtcagtg aacgggcggt ttccgccgtg atgtggatta
     9421 ttccattatc attcggcttt gttgaaagtg cgcaaagctc gaccgaattt atcgcctttc
     9481 gcacatcgcc accgcaagcg gaggaaatat gctttatcgc gtcgggatct acttcaaatt
     9541 tcattccgcg cttttcttca aggaatctga atccgcgttc aacggcagga acgatctccg
     9601 caggcgcaac cgccttaaat tcaaagaccg tcgaacggct gagaatggcg ttgtaaacgt
     9661 aaaaatacgg attttccgtt gttgaggcta taagggtaat tgaaccgacc tcaatatatt
     9721 caagcagtgt ttgctgctgc tttttgttga aatactgaat ctcgtcgaga tagagcaaga
     9781 tgccgttcgg agcttcaaag gtgtcgagct gagcgacaat ggccttgata tccgccgttc
     9841 ccgctgtcgt tccgttgagc ttgcagagct ttctgtccgt cgcctttgca atgattgaag
     9901 cgacggtcgt cttgccgact cccgacgggc cgtagaaaat catgttgggc agttctcccg
     9961 attctattat atttctcagc ggcatatttt cgccgataag gtggcgctga ccgacaacct
    10021 catcaatggt ttgcggtcta agctgttgag ctaaaggtat agacatttta tcgcctcccg
    10081 gttgcataca attatatatt aaaatgcgtg gtttgacaag gataaattta ttgactaatt
    10141 atgtattatg tgatataata actaaaattt ttttatgaaa ggagtttgtc cacaatggat
    10201 gacccgggtg ggattatact caagctgatt ttacttttat tacttatttt agtcaacgca
    10261 ttttttgcaa tgagtgaaat agcggtaata tccctcaacg ataacaaaat agaaaagatg
    10321 gctgaagacg gccacaagaa agcaaagcag gttgttaagc tgaccgaaaa ttcgagcaag
    10381 tttctctcga ccatacaaat cggagtaacc cttgcgggat tccttacttc ggcgagtgca
    10441 tcgcagacct ttgcagacat gatagtcaag gctcttaagg gcacggcatt ttataatgcc
    10501 ctcggtgcaa gcgtagttag cggactttcg attgttataa taacaatcat catgtcgtat
    10561 ttttcgcttg ttctcggcga acttacaccg aagaagatcg ctatgcagga ccctgaaaaa
    10621 atttctttca aggtagtcgg aattttgctt ttcttctcca aggccttttc tccgttcgtc
    10681 aaagtgctgt ccgtctcaac caatgcggtc gttcgacttc tcggctacaa tcctcatgcc
    10741 gatgaagagc ctcttaccga agaagaaatc cgaatgatgg ttgatgtcgg cggcgaaaaa
    10801 ggtgttatcg aagacgttca aaaagaaatg ataaataaca ttttcgaatt tgacgatctt
    10861 gatgcaggcg atattatgac ccacagaacc gatatgacgg cggttgacgt aaacgatccg
    10921 ataatggacg ttgtcaaaac tgcaattgaa tccggatatt cgagaatccc cgttttcgat
    10981 gacgacccgg ataatataat cggtatcatc tatgttaagg acttgctcag atatatagga
    11041 acggatcttc cgaaacacac catgagaaaa atcatgcgta ctccgatgta cgttccggaa
    11101 tccaagagct gcggtgacct tttcaaggaa atgacaagtc agcatacgca aatggctgtt
    11161 gttgttgacg aatacggcgg aacggcagga ctcgtcaccc ttgaggatat cattgaagca
    11221 atcgtcggaa acattcagga tgaatatgac gatgaagaag aggaaatctc aaagataaac
    11281 gaaacaacct tcactattga cgggttgaca gatatagacg aacttgacga aataatcggc
    11341 aaggatctcc ctgagggcga ttatgacaca atcgcgggtt acatcataag ccgactcggc
    11401 tatctcccga agcagggcga gcacagcgag gtcatgtttg aaaacataag atttaccgtg
    11461 cttgaggtcg aaaaccgacg tattgaaaag gtcaaggttg aaattcttcc gctccccgac
    11521 gaggacgatg aagaggaaga ggactcggac aaaaagaata aaaacaagga aaaagaataa
    11581 tttcaatgcc gcatccaaat gagtgcggct gttttttgaa aaaatttttg taaattatta
    11641 acgagataaa tatatgaaaa acggagataa aacgagaata agagagttcg cgaatgataa
    11701 attaaaaaat tgagaaaaag ttcttgactt agtaaaacgg ttgtgttaat atattcgagc
    11761 agtaaaaatt tatttggagg taagtttatg tcaacttata tgcctaagtc gggcgacatc
    11821 gtccgcaagt ggtatgtgct tgacgcagcg ggaaagccgc tcggccgtgt tgcggctgaa
    11881 gcagccgtac ttctccgcgg taagcataag ccccagttcg ctcctcatac agattgcggc
    11941 gatcatgtta tcataatcaa cgctgagaag gttgtcctta caggcaacaa gctcgatcag
    12001 aaaatgtact atcatcacac cggttacatc ggcaacatga agaaggttaa gtacagcact
    12061 cttatgcgta caaagccgac tttcgttgtt gagaaggctg ttaagggaat gatccccgac
    12121 acaacaatcg gtcgtgaagc actcacaaga cttcgtgtat atgcaggcgc agagcacaag
    12181 cacgctgcac agaagcccga agagtgggca ttttaatgaa gggaggcaac aataatgtac
    12241 gattctaatc catatttcta cggcacaggc cgcagaaaga aatcagtagc tcgtgttcgt
    12301 gtttacgcag gtacgggcaa ggtaaccatc aacgacagag acattgatga ttatttcgga
    12361 cttgaga
//
LOCUS       DAWBSW010000040        12229 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1127_length_12229_cov_2.759323, whole genome shotgun sequence.
ACCESSION   DAWBSW010000040 DAWBSW010000000
VERSION     DAWBSW010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_12229_cov_2.759323"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    868..877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3938..3947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7065..7074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9138..9147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3472 a         2407 c         2972 g         3338 t
ORIGIN      
        1 cctcatcacc gtcgccgtca taaaacgcaa ctccgccggc atcgagatcg ggcttaagaa
       61 cgcattgtct gccgttctta acaaagttga tattgagacc gtcaaacata tctgcgccgc
      121 cctcggtgcc gcaaatcatt gttattgcct ctttaggatt tctgatgttc aaagcccaac
      181 tggattcgag cattatcgtt gctccgtttt ccataacgat aaagccgaac gcagcgtcct
      241 caaccgtgaa tttttccgga tcccagtcgc cccaagcgtt gccgcagtcc ttttgatttt
      301 tgagcttttc aaaagtcgtg ccgactacca tcttcggctt gtaattatcc atcatccaaa
      361 gggtaagatc gagcgcatgg gtgccgatat caatgagcgg accgccgccc tgctcgtatt
      421 cgttgaggaa aacgccccat gtcggaacag cacgtcggcg aagcgcaata gccttgccgt
      481 aatatatttc gccaagctcg ccgtcctcac acattttttt gagatagagc gaatccgctc
      541 tccagcgtga ctggtatccg atagtgagct tcataccggt tctttcggaa gtgtcaagca
      601 tttctttggc ctctttatat gtctttgcca tcggcttttc acacattaca tgcttgcctg
      661 cttccatggc atcgattgaa ataaaagcgt gtgagcggtt cggcgtgcag acgtgaacaa
      721 cctcaatatc cttatccttg agaagctcct tatagtccgt gtagaccttt gcgccttcaa
      781 tgccatattc ttcagccgct ttgaccgctc tttcttcgat aacatcgcag aaagcaacca
      841 tttgagcatt ctttattttc ttgagcgnnn nnnnnnnatt atacctattt ttactgttct
      901 gtccatttta aaactccttt attcggtttg ttttatgata tcatattgag tgaaaaagtc
      961 aagataatcc gttcgcaaaa tagaaatacg cccttgcaaa agcggcactt tattttttgc
     1021 gaatcttatg atacaataaa gcaaattaaa ggcggtgaag atatgattaa aacagtgatt
     1081 tttgaccttg acggtctgct ggtcaacagc gaattgatga cttatgaatt ttataaaagg
     1141 atacttgctg aaaaggaaat tgaattttct ttaaaaacat ataccgatgt ttattgcgga
     1201 cgaacggctg tacgtaatct aacggaattg attgaaaact atgatttacc gtttgatgtg
     1261 gaaaagggct tggagctaac tcatgaggcg gagaaggaat acctaagcac gaagggtgtt
     1321 ccgctcaaaa agggcgccaa agagcttctt gactttctca aagataaaga ctacggtatc
     1381 tatcttgctt cgtcgagcgg aaaagagcga gcgcatacga ttctttccgg aaacggcata
     1441 gacggatatt tcgacggcgg agttttcggc gatgaaatag agcacagtaa gccggcaccc
     1501 gatgttttcc taaaagcaaa agaaaaggca ggcgtaaggg cagaggagtg tcttgtgttg
     1561 gaggacagtg acgcgggaat ccatgctgcc tatgccgccg gaattcccgt tatctgtatt
     1621 cctgatctga agcagccgtc cgaggagtca agaaaaatgg caaagcttgt tcttccatct
     1681 ttgcttgacg ttaaagaaat gcttgaaaag aaagaaatat aaaaaaaggc tgtggcaaaa
     1741 tgaactttgc cacagccttt gctgttgttc tttaaggtaa taccatacaa ttgagatgtg
     1801 caaattgcgt ggtataatta ttcatcatgg atgaagctga gctcagagga gctttattct
     1861 attcttcaat ctctctaata acttttttac tgcaggcgac gaatttttga acgatgagag
     1921 gcattgtatt cttggacttc acgataatgc cgaggtcacg gtgctcatcg gggtagagag
     1981 gctttgtgat gacatttcct ttcattccgt caagaatgag cttcggcaat atgctgtagc
     2041 ccaagccgtg gctgacgatt gaaacaacgg ttgcatcgtc aacagtcgtc gagtttatgt
     2101 tcggctcgat tccgctttcc tgaatgattc tcaagatatc accgtctgca ccgtaggtag
     2161 gaatgaaaaa tgatttgtcc tgaaaaaagt ccatcgtaat gtaatcgtca gagttagtgt
     2221 cgttcggcgg aagaactgca aaaatacggt cgtcaagcag gtgaatccaa tcgaatttat
     2281 tgtttttaat aaggctcata aagccgaggt caacctcact gtttgcaaca agacgtgaaa
     2341 tttcctccgg caagccgacg tgaagctcaa attttgcatt tggattttct ttgcgaaagc
     2401 tgcttataat aacagggagc caatggttcg caatgcttga atatgcggcg attcttatta
     2461 ccttgttgtc gtccgccttg atgtttccga ttgccgtgtc gagagccgag gcggcatcgg
     2521 tgaagttttt gatatacggc aaaagatcct tgccgtcggg tgaaagcttt actccgagtt
     2581 ttgttcgctg gagaagcgaa acaccgattt ccttttccag tcggttcatc atatgcgtga
     2641 gtccggcctg agtgtaaccg agctcttcgg cggctcctgt gagactgccg cattctattg
     2701 cttttaaaag agcatgaaat ttctctgagt ccatatagtt atcattccta acgtaataac
     2761 attttgtaat agatatatta taatatgtag gtttactttt ttcaagtttt attgtataat
     2821 ttttctacaa tttttggatt ttttaaaaag agaggggatt taattcacat ggcagaatta
     2881 aaaaagaaat acggtttgat caccgctatc tgcatggttg tcggaatcgt tgtcggttcc
     2941 ggtgttttct tcaaggcaga ggcgatgctc aacatcacaa acggtgacct tccggttgcc
     3001 ttgctctcgt ggcttatcgg tggctgcgtt atggtaatca gcgcatatac ttttgccgtt
     3061 atgggaacaa aattcgagaa aatcaacggt gttgttgact attccgaggc ccttgtcggc
     3121 agtaagtatg cttattatgt cggatggttc atggctacga tttactaccc ggcaatgaca
     3181 tctgcgcttg catggctttc agcgagatat acaatggtgc ttttcggatt tgagttgaac
     3241 tctgcggaaa caatggttct cgcatttgtt tatcttattc tcagctttgc gcttaatacg
     3301 ctttctccga agcttgcagg tcatttccag gtttcaacaa ccgtaatcaa gcttattccg
     3361 cttattctta tggctgttgt cggaacaatt tacggaatca aaaacggagt tcttatagat
     3421 aacatgaatt ctgttgttga acttgttaag gacgccgagg gaaatattgt ttcaagcaca
     3481 caggttgcaa ggcaaggcgt ttcaacgcat cttattcttg ccggtgtttg cacatctgtt
     3541 tttgcttatg aaggctgggt tatcgctacg gctatcaact ccgagcttaa ggacgcaaag
     3601 aagaatcttc cccgtgctct tgttctcgga acaattatcg ttgtcgttgt ttatatgctt
     3661 tactatatcg gaatttcggg cagcatcaag actgccgagc ttctcccgaa cggtgcaggc
     3721 cttgcgttca agacaatttt cggaaacgtt gccggaacaa ttttgactgt attcattgca
     3781 gtttcatgtc tcggaactct taacggactt actcttgcgt gtgaccgcgg attctattct
     3841 cttgcggcaa gaaatcacgg accgagaccc gatttcctcg gcaagcttga ccatgccacg
     3901 gagatgccct caaacgcttc gatcgttgcg cttatctnnn nnnnnnncgc ttatctttaa
     3961 tgcgctttgg ctcttctatt tctacatggc taaccttact ccttttgcaa gctacacggg
     4021 aaagggctct atattcctct ttgactctac agagcttccg cttatctgtg tttacgcatt
     4081 ctatcttccg ctttatgtta tgctcatggt aaagggaaag gaattcaacg ttttccgtcg
     4141 ctttattatg ccgattctgt cttttgcagg tgcatgcctg tttatctatg ccgctattgc
     4201 aaagcacggt ataaagaaca ttggcttcat tattgttgcc ggagttatca tgcttctcgg
     4261 agcaattctt tataatccga gaagtgtaaa gcaaaaagca aaaagcaaaa aataaaaagc
     4321 aataattttt gaaaagcctg cggtgtaaac tgcgggctta tttattgaca gagggcattt
     4381 tttggagtat cattaaaagc gaataaacag taaattattc agcaaaggaa atgaatacta
     4441 tgaaattgaa tgaactggct gtcggcaaga gcgccgctat actgagggtt ggcggtaagg
     4501 gcgctttgcg tcagcatttt cttgacatgg gtatgattcc cggcgccgag gtcacggtta
     4561 taaagcttgc gccgatggga gacccaatgg aattgcagat tcacggctat gagctgacat
     4621 tgagactcgc cgaagccgaa cagatcgaaa ttaagcctat acaaaagatc aagagcagat
     4681 acaaggatac agaaagaatc aaggcgtcgg agcaccccgg actgggtgag gacggtaaat
     4741 atcactcaaa ggcggaggaa aatccgttgc ccgacggtac aaaattgacc tatgctcttg
     4801 taggcaatca aaactgcggc aaaacgacgc tttttaatca aattacaggc gcaaaacagc
     4861 atgtcggcaa cttccccgga gtaacggttg accgcaagga cggaatgatt cgaggttatc
     4921 cgaacactga ggtgacggat ctgccgggaa tatattccat gtccccgttc agcagtgaag
     4981 agattgtatc aagaaatttt gtgctcaatg ataaaccaaa ggcgataata aatatcgttg
     5041 acgcaaccaa tatcgagaga aatctttatc tgacaatgca actgcttgag atgaatgtcc
     5101 cgatggtagt tgccttgaat atgatggatg aggtcgtcgg taatcaaggc tctatagacg
     5161 ttaacgctat ggaagctatg ctcggcgtac cggttatccc gatttctgcg gctaagaacg
     5221 agggcgttga cgagctgata aagcacgcga tacatattgc aaagtatcag gaacgaccgc
     5281 tcaaacagga tttttgcgac aagaacgacc ataacggtgc agtccacaga tgtattcatg
     5341 ccgttgttca tcttattgag gatcatgctg caaatgccga gctgcctgtg cgttttgccg
     5401 caacaaaggc aatcgagggc gatccgctta ttcttaacca gctcaagctt gataaaaacg
     5461 agcatgaact gcttgaacac attgttcagc agatggaggt tgagtgcggt cttgaccgaa
     5521 gtgcggcaat agccgatatg cgctttgatt ttatcgaaaa gctctgtgaa aaaacggttg
     5581 tcaaaccgag agagagcaag gagcgaataa gaagcgaaaa aatagacaaa atacttacag
     5641 gcaaatacac cgcgatcccg tgcttcattg cgataatgct tctcgttttt tacctgacat
     5701 tcaacgtaat cggtgcgtgg cttcagaatc ttttggcaat gggaatcgac aagctctcgg
     5761 aaattacaga tgcggcactg accgcagcta atgtcaatgc ggcagtgcat agcctcataa
     5821 ttgacggaat tttcacgggc gtaggcagtg tgctgagctt tttgccgata atcgttactc
     5881 tgtttttctt cctctcaatg atggaggaca gcggctacat tgcaagagta gcatttgtta
     5941 tggataagct gcttcgtaaa atcggacttt cgggcaagag catagtgcct atgcttatag
     6001 gattcggctg taccgtgcct gcggttatgg caacaaggac gctgacgagt gagcgagaca
     6061 gaaaaatgac gattttgctc actccgttca tgagctgttc ggcgaagctt ccgatttatt
     6121 cgttcttcgt cagtgagttc ttccccggca agggcggact gattatggcc ggtctttatt
     6181 tccttggaat tttgctcgga attttggcgg cgtttttgta taaaggaaca ttgttcaagg
     6241 gcgagccggt tccgtttgtt atggagctgc ccaactaccg tctgcccgga ataaaaaatg
     6301 tttcacaatt gctttgggaa aaagcaaaag attttttgca gaaggctttt tcaattatac
     6361 ttatcgcaac ggtggttgtt tggttcctgc aaagctttga tcttcatttc aacatggtta
     6421 cgaattcagt ggacagcatc cttgcggcga tatcgggatt gattacaccg atattcaaac
     6481 cgctcggtct cggcgattgg agaatttgca cggctcttat cagcggattc atggcgaagg
     6541 aaagcgttgt ttcgacaatg caggttcttt tcggaagcgg tgttgcagcg gtgctcactc
     6601 cgcttgcggc agcttcaatg cttgtattca gccttattta taccccttgc gttgctgcga
     6661 ttgcttcaat caagcgtgag cttggcttca agtgggcggc aggcgttgtt atttggcagt
     6721 gtgttattgc gtgggtagtg gcatttctcg tttatcttat aggatcggcg gtcggaatat
     6781 aatgaatatt tgggatattg tgatagttgc gatccttgtg cttgtaattg ccggtgcggt
     6841 atttacaatt atcaaacgca agaaaaatgg cggctgctgc ggcggaagct gcagtcaatg
     6901 ctcatgctgc aaggagaaaa acaaacacgg ttaataatta aaatgaggat tgtcatatcg
     6961 gcaatcctta tttactttct attgattttt tggtagaata ctatcagaac aaattcaatc
     7021 gaggtagcta tgaaaaagat cgttgcagga attttggctc atgtnnnnnn nnnnggtaaa
     7081 acaacgctcg ccgaagcgat gctttaccgc acaggaaaat tacgtaaaat cggtcgtgtc
     7141 gatcacggtg acacggctct ggatacccat acccttgagc gtgaaagagg aatcactatt
     7201 tttgcaagtc aggcggtttt ttcgacggat aaaatcgagg ttacactgct tgatacgccc
     7261 ggtcacgttg atttttcctc cgagacggag agaacactct ccgtgctcga ttatgctatt
     7321 cttgtcatca gcggacttga cggtgtgcag tcgcacacta tgactttatg gaagcttttg
     7381 aagctctaca acgttccgac atttgttttt gtgactaaaa tggacttcgc acgaaagagc
     7441 cgcgaggaga taatcgaaaa ccttaattcc gagcttgacg gcgaatttgt tgatttcggc
     7501 gacgaggaag ctgtgtcgga gaacatggct ctttgcagcg agagcctgat ggagaaatat
     7561 ctcagcggcg aagaaataga tgaaaaagaa atagccgaag caattaaact gagaaagatt
     7621 attccgtgct tcttcggctc cggcttgaag cttgacggta ttgacaaatt cataaaagct
     7681 ctcgaagaat atacaattca gcccgaatat cccgaagtct tcggcgcaaa ggtttttaaa
     7741 ataagtcatg attcgcaagg cgtgcgcctg acccacatta aggtcacagg tggctcggtc
     7801 aaggtccgag agatgatagg cgacgaaaaa atcagcggca taagaatata ttcgggtgca
     7861 aaatttacaa cggcagacga ggtgggatcg ggcgaaatat gcgccctgac ggggcttgat
     7921 aaaacgcata acggacaggg gcttggcttt gaagatgcag gggagaagcc gacgcttgag
     7981 cctgttatga attaccgtgt tgtgcttccc gacgggtgcg atgcagatac gcttctgcca
     8041 aagcttcgtg agcttgagga ggaggatccg cagctccatg taacatggaa cagtcacctt
     8101 aaagagatcc atgtcggact gatgggcgag gtgcaggcgg agatactcaa aagcattgtt
     8161 gccgagcgtt tcggcgtgaa aatagatatc gactcgggca gggtcatgta taaggagacg
     8221 atagagaaca ctgttgaggg cgtcggtcac tatgaaccct tgcgccatta tgccgaggta
     8281 catcttatca tggagccgtt gccgagggga gccgggctta tctttaaaac cgattgcagc
     8341 gaggacaccc tcgaccgcaa ctggcaaagg ttgattctta tgcatttaga cgaaaagcag
     8401 cacctcggag tgctgacggg ctcaccgata acggatatga aaatcaccct tgcggcaggc
     8461 agggcgcaca ttaagcatac ggagggcggc gacttccgcc aggcgaccta cagagcggtg
     8521 cgtcagggtc ttatgcaggc aaaatcaaag ctgcttgaac cgtatttctc atttagactt
     8581 gaggtgccga gcgagcagat aggcagggcg ataaacgata ttagaatgaa gtcgggaagc
     8641 tttgaatcac ccgaggagag cggcggaatt tccgttctca gtggcagagc gcccgttacc
     8701 gagctgaacg gctatgcatc cgaggttgcg gcttatacgg gcggcagagg ccgtctctat
     8761 tgcgagagcg caggctatga cgattgtcac aatgccgaaa aggtgatagc cgagcttgcc
     8821 tacgatcctg aggcagatct tgaaaacaca cccgattccg tcttttgtgc tcacggaggc
     8881 ggatttggcg tgaagtggaa caaggtcgga gagtatatgc accttgaaag ctgtcttgaa
     8941 aaagagaagc cctacactcc gcccgtcaat cgccgaaacc tgcacattga cgataaggag
     9001 ctggaggcta taatggagcg agaattcggc aagccgaaat atgagcttta cagaccgacg
     9061 gcgaagaaaa atgacgaaaa tcaaacggat tttgagctga ccgagcgcaa gagctatgtc
     9121 ctcgttgacg gctacaannn nnnnnnngag cggctgatgg aaattctttg caattacagc
     9181 gcctacacga agaacaacgt cgtgcttgtt ttcgacgcat acaaggtgcc gggcaacacg
     9241 ggcgagcgat tcgattttca taatatccat gttgtttaca ccaaggagag ggagctgggc
     9301 gacgtttata ttgaaaagct catatcggaa atcggcaaga acgaccgggt acgggtcgtt
     9361 acgtcggata atcttattca gctttcggcg gttcgcttcg gtgtgctgag aatgtcggcg
     9421 gcggagtttg agcgtgaggt agattccgtt cacgccaaaa tcggcaaatt cctcgacgaa
     9481 atcagagaga aaaattctaa aacgaagatt gatgaaattt caaaaatctg aaatcgagcg
     9541 atagattacc ttaaaagggg atttatgaat gaaaaattta tgtgagcaaa tgtggagtga
     9601 aaactgccga tacataagaa atctatgtga atataagctc agtgattcac acaaggctga
     9661 tgaatgtata agtgaggtat atacagcttt ggtgcaggca ttagagaata aaactgaaat
     9721 attgaatcca aaggcatggt taacagctgt tgcaaagaat aaaataattg atttttatga
     9781 ttcggacagg aagaacaatg ccgttgttgt ttcgctgtct gctttcggcg agtcggtagg
     9841 ttcgcatgac agctatagct ttgataaggt ctctgatgat gagatagaac tgattaaaaa
     9901 taaagtgctt gaacgtcttg atgaaagaga aaaagtaata attgaggact attatcaaag
     9961 gggcaagaaa atcaaggtaa ttgccacaga gagaaatttg agtgaagtga atgtcaaaca
    10021 gatacttttt cgagcaagag aaaagataaa aagcaatgca aagaaagaat tgaatagaca
    10081 tattgaacaa agaaaagata aataatttgt gcaatatttt ttgtaacctt ttgatttttt
    10141 ctcgactata taagcaaagg agaaacgttt atgacaaaca acgaattcag aaatattttg
    10201 aatgacgaag aaaatttact gtcaaaagaa gaacttgagt tgttgataaa tgctgaactt
    10261 gccaagcccg aagaagagat ggataccgag cttatagatt attgtttaaa ggcacttcag
    10321 gcatttaata atgacgataa aaaggaaagg gacggtgaaa taaagactaa aaagaaaaaa
    10381 cgtgtattaa cgaggataat tgcaattgcc gccgcagtcg cagttataat tgcgggagca
    10441 gtgtcggctt cgacgggaat gcacaagacg aatgttaagg aggggattgt ggaattttac
    10501 agggattata tacagatcaa tttcagcggc agtgagaata gaaagcagga atttaaatca
    10561 tcagattcaa agctgataaa ggagcttgcc gacaatggca tatcgcctgt ggttttgccc
    10621 gaggcactgc tttcggagga ttacaaaata gaaaggcttg agtatgaaga caccgaatac
    10681 attaaatctg caaacataat gcttaagcaa aaaaggaaaa aagtaaacat tacgataatc
    10741 agatatgatg aagcaatagg attgcctgat atggaatata tgaataccga gggactggaa
    10801 aaaattacgg tagacggagt agatgtatat gtcattataa gagatgatga ttataaggtc
    10861 atcacatatg ttgacagaga catgtattac agtatcgtag ccgagtggga tattgaaacg
    10921 gctttggaga tagcaaaaac gataaagtag gaggaaaatt ataatgaaaa aaataaaaat
    10981 gataatggca gtgatatttg cattgattct gttgacggta agctatgcga ttcctgcttc
    11041 attataaagt gtatgaaggt aaatcaaatg agaaatttaa tctatatgta aatatgggct
    11101 gtgtcttaca aggaaagagg ctattgggtt catattctca ttcggttata gttgttttgg
    11161 taggcagatc aatacgatat tgtgctcata ctacagcacg aaaagatgag cctataaaaa
    11221 cgttctatga tgggtttaat aaatgtagag ctatagaggt ctaccaaact tattaaaaag
    11281 agggtaaatc aatgaaaaaa attattgttc cgttgctttg cattttgttc cttttaacct
    11341 catgcagcgc aggtacggat aacctgaaaa gttattggaa ttacagagaa tatttcggta
    11401 tatcatatct gactattatg cataaaagct cggacaaaga aacagaaatc ggtgaatctg
    11461 ttttagaaac ggctcaaaag gtttttacat atgtcggcga cgaaaaagat gctgataaaa
    11521 atgtaggaga attatacagg tattatgcct ttgtcaaagc cccgttagag gttaaaagca
    11581 gtgaagcaaa attagagttg attacaacta aaattaccgg aaaccgaggg gaaatgtggg
    11641 tggaatactc cagaatggcg gcatatgcga atgaagaagg cggctacggc ggtgaggcac
    11701 tgtgcctttg gaaattgaaa aaaacagacg gtaaatggat cgttaccgga gttaaagaaa
    11761 cggcataata taccaaacaa gcggctgcat tttcgtacag ccgcttgttt gttacagtga
    11821 tttatttttt tagcatttca taaaccgtct gatatttttc ctgagaatag tccttgtcga
    11881 gtgagaaatc ggaattgcct gcgatttcaa cgacatcttt ctgactgccc ttgaagaatt
    11941 tcttcgcata ggaatttacc tgataaagag cttcggcata aacgtcctcg tcctcaaccg
    12001 ttgcggtgag cgatttgata tcgtcactca gcgtgtcgct ttcggcaagg ttggagtttg
    12061 agaaagcaat caatcgaagc gcaaggcttg ccgcctttgc cgatgagcct gccgtgccga
    12121 tagggaaatt attgatatcg gcaaagatat ctttgatcgt atcaatcttc tgaggagcct
    12181 cggttgtcgt ttccgtttca gtctctgccg tggtctcggg ctcggtcgg
//
LOCUS       DAWBSW010000041        12225 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1128_length_12225_cov_2.912736, whole genome shotgun sequence.
ACCESSION   DAWBSW010000041 DAWBSW010000000
VERSION     DAWBSW010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1128_length_12225_cov_2.912736"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    901..910
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4888..4897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7765..7774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3501 a         2476 c         2894 g         3324 t
ORIGIN      
        1 ttaatattta ttttaaataa tcctgttaaa tctgttttga ccttttcgtt gctgttttgc
       61 gtgttgctgc catagtcggg aggcaagaag atttctgtct tgaaagcaat ttctgtcaga
      121 cctccaaggt gcgatgtggg catcgaccca aggtcggaag aaaacaatct cgaccctgta
      181 ggggagccgg ccggaaatta tccttataaa agaaaaaagg cttgcgccga aagcacaagc
      241 ctgctgttta gatgttttat cttttgctgt attgctcaat gaggtcattt ttgacgtccc
      301 aaaggcgctg agaaaggtca acgtaatagt tatggggatt ttcaaatctt gtaacctcgt
      361 cccaaagagt gtcgacctga tcaagacagt attttttgat atcgttaata tcctgatact
      421 cgtaaactct ttttccgtta atgaaaagcg gttgaagaag ctgacgtgca gtaaagttgt
      481 agacattttt gcgcttccag gtgtaatcgg ggtcgaaaat ctcatagtcg tgactgtcgt
      541 caatagtctc atcctcaagg gtaataacat cggcaagagc cttgcccgat tcattatcgt
      601 agagtctcca aagctttttc gcacccgggg ttgtaacctt gacggcgttc tcgctgagct
      661 tgattttcgg aaccattttg ccgtcctttt caactgcact gagcttatac actcctccga
      721 aaacgggggc agaagatgct gtgataagtc tttcgccgac gccgaaagaa tcgacctttg
      781 caccctggag aagcatatct cttataatgt attcatcaag cgagttagaa gcaacgatct
      841 ttacatcctc aaaacctgct tcgtcaagca tgatccttgc tttcttggaa agataggtaa
      901 nnnnnnnnnn tgtctccgct gtctattcgg attcctttcg ggcggaatcc gcggggcaga
      961 acctctcgat taaaggcttc aatcgcgttc ggaataccgg atttcagaac attgtaggta
     1021 tcgacaagga gggtgcagtc atcgggataa gcctttgcat aagcgcagaa tgcctcaagc
     1081 tccgaatcga aaagctgaac ccaactgtga gccattgtgc caagcgccgg aacgttgaat
     1141 tcacggtcaa ctattgtaca agccgttcct gcacagcctg caatatacgc agcccttgcg
     1201 ccgtaaacag cgccgtcaaa gccctgagca cgtcttgaac cgaattccat tatcggtctg
     1261 ccctgagcgg cacggcaaat tctgtttgct tttgttgcga taaggctctg gtggttgatt
     1321 gcgagcaaaa ccatcgtttc gatgaactgc gcctgcataa ccggtccctt gaccgttacg
     1381 atcggctcac ggggaaagat gggagttcct tcgggaacgc tgtatatgtc gcatttgaat
     1441 tcaaaattct tgagatagtc gaggaattcc tcgcagaagc ccctgcctct gagatattca
     1501 atatccttat ccgaaaagct cagattttcg agatattcaa cgagctgttc gacgcctgcc
     1561 atgattgcaa agccgccgtt atcgggaatg gttcggaaga acatatcaaa ctgtgcggtc
     1621 gtgtcgccct ttccgttgag gaaatagccg ttagccattg taatctcgta aaaatcagtg
     1681 agcattgtaa ggtttctttt tttaagatcg ttcatatttg caccgccttt tgggttgtta
     1741 tcgacattat aacaccgaaa accgtctttt tcaatcccat atttataaaa gggttatgtt
     1801 aattgtataa caaaacggga ttttaaaacg caaaaatcgt taaaatgtat aaaaatgatt
     1861 tgcagttgat agaatatggt ctataaataa ctaaaaaata tggtattacg ttttatttgt
     1921 tgttttaaaa gttgctttat gttattatat agaaagaaaa aatatgtttt tagcataaat
     1981 aggaggaaaa ccatgagaac aagaaaacag gcgcttggcg acatgaacat tatcggcgct
     2041 aaggttgaac agcagagaaa agcaatcgga atgaagcaga aggatcttct tgctcagctt
     2101 cagatcagag gtattgacct caatgcttcg ggtctctcaa agcttgaggg tcagctcaga
     2161 ggagttaccg attatgagct taaagcaatc gcagagtcac ttaacgtcag ccttgaatgg
     2221 cttctcggct gcgaggaata attacggaac tgccgcattc ggcgcagaac gaagagcaaa
     2281 ttaacaagaa taaaagctat aattcggttt attgctgtca gaagactgaa cacgaattat
     2341 agcttttgct ttgctcttct aagcattttc aactccctcg cagtacagtt gtacagcttc
     2401 ggagtgagaa aatgctttct gcatctgaat gagacagctc catatgtgac agtatgttct
     2461 cgatttatta ttgacaaagc tgaggtaaat atagtaaaat taaatttgat aaaaacggtt
     2521 tggggcagcc ctgaccgtat gaacaaggag cagtgaatat tatggcagtt tttagaccct
     2581 ttaaggcgat cagaccgatc cccgagagag caaaagatgt tgctgcattg ccttatgacg
     2641 taatgaacag cgacgaagca agagaaatgg ttaaaggaaa tccctattcc tttcttcatg
     2701 ttgataaggc ggagatagac cttgatccct cgatagatct ttatgacgag aaggtttatc
     2761 tcaaggcaag agagaacctt gacaagcttg tgacagacgg cgtttgcaag gaggacgaga
     2821 ctccgtgcat ctatatctac cgccaggtta tgaacggcag aagtcagact ggtcttgtcg
     2881 gttgtaccgc tattgacgat tatatcaaca acgttatcaa aaagcatgag cttacaagag
     2941 ccgataaaga ggcggacaga atcaaccacg ttgactattg cgacgccaac acaggcccga
     3001 tattccttac ataccgccct gacgcattca taagcaagac cgttgaggct tggaaggaga
     3061 accatgctcc ggtttacgat ttcgtctccg agggtgttga aaacacggtt tgggttatcg
     3121 acgatgccga cacagtcaag gctgtcacgg ataagtttgc tcagatcgag tctctttaca
     3181 ttgccgacgg tcatcatcga gcagcttcgg cagttaaggt aggcaagaaa cgccgtgagc
     3241 agaatccgaa ctacacaggt gaagaggagt tcaacttctt ccttgcggtt cttttcccga
     3301 ccgatgagct tgaaatcatg gactacaacc gcgtaatgaa ggaccttaac ggaaatacat
     3361 ctgatgagtt tatggctaaa ctcggagagg ttatggatat cgaagaatac ggcaaggacg
     3421 catacaagcc cgagaagaaa cataacttcg gtatgctgct tgacggaaca tggtacaagc
     3481 ttactgccaa ggacggcaca tttgatgcaa acgatcctgt tgatcagctc gatgtttcga
     3541 ttcttcagaa taatgttatt tctcccatct tcggaataga cgatccccgt accgacaaga
     3601 gaattgattt catcggcggt atcagaggac tcggcgagct tgagagaagg gttaataccg
     3661 atatgaagat cgcattctcc atgttcccga caacgcttga tgaccttatg gatattgcgg
     3721 atgcaggcaa gatcatgccg ccgaaatcca catggtttga gccgaagctg atgagcggac
     3781 tgtttattca taagctttca taattcagga cagtctcacc cggcacagaa cgaaaaaaat
     3841 aaatataggc cataatttac aatgaggtta gaacgttagt tgtaaattat ggctcttatt
     3901 ttttattgta tctgtatatt gcgtgacctt ttctttgatg atataatgaa aatataaatc
     3961 ggacaggaga gggttatatg aagaagatta ttgcggttat tctcagcctt attattgcct
     4021 tgactttaag cgtgcagacc tttgcggtcg aggtcagacg tgatgatttc tatcctattg
     4081 taattgtgcc cggttattcc tcatcggggc tttacatgaa caatgaggac ggcactaaaa
     4141 ctcatgtttg gggaattgat acaaatctga ttattaagag ggtgcttgcc cgttctatag
     4201 accttgctaa gggcatcggt aagctgacaa agggcaacgc aaaatatatt gcagacatcg
     4261 tcggcggaga atttgcggaa atgtttgagc atatgcgctg caatcccgac ggctcaagcg
     4321 tttatgatgt tcatacatac acgtccacgg cggcagattc aagcaacacg tatctgcttg
     4381 caaatgagga cggcaagtat atgtatgagc aggaaatcat gggtatgtac ggcagctata
     4441 tcggcgagga ttggaacgat tatattttta atttcagcac cgatttcaga atgaatgttg
     4501 aaaagtgcgc cgctgacctt gacaaatata ttgacagcgt gctcgaatac acgggtgcgg
     4561 aaaaggttaa tctttattgc gtcagccacg gcggacaggt cggagcgaca tttctcaatc
     4621 tttacggaga aaagaaagcc gacaagctca ataacgtttt gctgaccgtt cccgccatcg
     4681 gcggagcttc tattgcatac ggatttttca cgggtgacat aaagtttgac gaggaaaatc
     4741 ttctttattt catcgaaaac ggtatgatgt ttgagaatga ctatcactgg ctgcttgcaa
     4801 atgagaatct tgattttatt gatgatattc ttcattattt tcttctttta atattttatt
     4861 tctctttttg tttgctttta aattatcnnn nnnnnnnata ttcttaatta ttttcttccc
     4921 tatgtagatc atattatgga gttttggggc agcatgtggg attttgtccc ctatgaggag
     4981 tatgacaaaa ttcgtgacag ctatcttgac agcaatgctt ctgccgatct gatagaaaga
     5041 tcagaccgct ttcatcacga aatctatccg agtatgacgc agaggcttcg ggcgtgcgtt
     5101 gatgccggaa taaatgttta tataattgcc ggaacgggca acagggatat tatcggatac
     5161 aaagcaacag gcgatgcgat aattcccact tcgtcctcaa ccggtgcaac atgcgcacct
     5221 tacggcaaaa ggttcaataa tggctataag acgcttggca cggtttgtga cgatgaagct
     5281 cacaaccacc tttcgccgtc aatggagatt gatgcttcga cgtcctatct gcccgaatac
     5341 acatggttta ttgacggctt ttatcacgga atgacgataa agagtgatta caatacggag
     5401 cttatcacaa cccttatgta taatgacgag cgtgccgatg tttataccta tcccgaattt
     5461 cctcagttcc atgcagacac aaaccgctgt caggctgttt ttgcaaagtt caataacaat
     5521 gttgagggtt atgtttcgga cagcgataac gccttgataa tcgaaaattg ttcaaagaaa
     5581 tatgatttga gaattgtatc tgtttcttgc aacggtatgg atataaagat tgataaaagc
     5641 tttatgacag aaattccggt cgggggaaga attgagattc cgttcacagg cgatatcccg
     5701 aatgcttccg gggtcaaaac acaggtgacg gtcaattacg ttcaggtcgg ggcggttacg
     5761 ccgttcggtt caaggacgtt tgactttaca ataatcaacg gagataaagt tgacttcgac
     5821 agcgaaaatc cgcttgtcaa taccgatttg ccgcaggacg ctattcttaa aataatactt
     5881 aaatttattc cgtctctcgg cttgcaaaag ctgaccgagc tggtgtataa taccgttaaa
     5941 tcattgtttt taagaggtgt taatgcatga aaagcaatat cacaagagat gaggcctttg
     6001 aacttctgag aaagtacaac agcgaaaaat ttcacattca gcacggtctg accgttgagg
     6061 gtgttatgag atggtacgca aacgacctcg gctacggcga tgatgcggaa ttctggggca
     6121 ttgtcggttt gcttcatgat atagattttg agaagtatcc cgaagcgcat tgcatcaagg
     6181 ctcccgagct tcttcgagag gcgggggccg gcgaggacat gatccatgca atttgctccc
     6241 acggctatga tctgacggtt gatatcaagc cggagcatga gatggaaaag gtgctttacg
     6301 caacggacga gcttacgggt cttatcggtg cggcggcgct tatgagaccg tcaaagagcg
     6361 ttatggatat ggaggtcaag agcctcaaaa agaaatataa ggacaggaaa tttgccgcag
     6421 gctgttcccg tgacgtcata gaaaagggtg cggaaatgct cggatgggag cttccgacgc
     6481 tttgggaaaa gacgattgaa gctatgcgct catgcgaagc cgatatcgca aagacaatgg
     6541 aaactatttg acaatttaat aatattattt cagcacatct tcgggtgtgc tgtttttttt
     6601 atgtcagaaa aggtcgccgc agggcggaaa aaaccgaaaa agctctgttt tgagtaaaaa
     6661 agattttttg gaattattat tggtgtgtcg gttgaacaaa accgaaaata aaactatcgg
     6721 gaggataata tgaacacttt ttccgaaaaa atcatatccg ttgaggaggc cagaaccgcc
     6781 ttgcgatgta tgaagctcgg cgatttgtcg gaggatgaag ctttggaatg tcttgacgaa
     6841 tttgttttca gagttcgcaa cgtcacaacg gcaaaaattg ttgaagaaat catcgaaaag
     6901 gagctgactc cgctgcagac tgaggtaatg aaaatgtatc tctacgataa tatgagagtt
     6961 gcgcagatcg gacgtgtgct cgggctgtca caggcggctg cttcgaaaat cgttgtaagg
     7021 gcgaacaaca ctatcgttaa gctcatgaca ccgcttatta agtatcagag tgatatctcg
     7081 gatgctcagt tggtgccggt taaggtcggc aagcttttgg atatctgtgc ggcgagaaac
     7141 ggaaacgcag cgtctttctg cgaggagctg agaaatttac gaattgcata tgatatcggc
     7201 gcagaacagc tttcgtcaaa cctgaaaata agcaaatggg atttggctgc aattgaggaa
     7261 ggaaggaaga ttccgtcaat aaccactgcg atgagatatt cggcgctttt tgatgttgag
     7321 attgaaatga aattcaaaaa cgggaggggc ttctattcgt gcaaaagacc ttagaggagc
     7381 ttggcaaaga atatctcgaa tctgcaaaga caattgaagc tctgataaaa aagtacaggg
     7441 gaatactgag ggaagcttac agctcgggaa attacttgaa aacatatgat accaaacgca
     7501 ggcttacgat tttttatgat cagaagcgtg agcttgaggc aatcggaaac aaactgataa
     7561 attattataa caaggaggat aattctgatg aattacatta atatgtcggt cgagggcaat
     7621 cgacttagaa taaaagagga aaaaatatcc gtcggaggaa gtataaacta tgaccgttgt
     7681 aaatttactt tcaatgacga atgggtcggc tttacgaaga cagtcgtttt ttcgataaac
     7741 ggagatgaga gctaccgtgt tgcgnnnnnn nnnnaaggag ggcattttgg aaatcggcgt
     7801 tttcggcgtt aatgataacg atgtcgtaat tacaacaaat tccgttgctc atcatgtcga
     7861 aaaagggctc aatatggtgg gcgagtggat tgaaggagac ggtgaatttg ttattaacgc
     7921 cgtggaggaa atgcgacgtg cggtcgatga gtacaccgag actctttcaa aaagagtttc
     7981 cgaggagatt gaccgattga aaacgggcag cgattcggat tccggtgctc cgacaccgtc
     8041 ggaatggtat tttccaatag cgtttaccga caccgaggcg gtttccccgc tttccaaaag
     8101 cggagccgaa tatgagcagt tccttgattt cagactcaat gccctgcttc gagacttccc
     8161 ggattacgta acgagaaagg aattgggcac ggacgcaagt gaggaatata tgatgtattc
     8221 gtattcgttt gcgccggcac gctatgacaa gacgattttt attgcggcat gtctgcacgg
     8281 ctcggataaa tgcggcttgc ttgcggtttc acacttcctt gattgccttt gccgcgaata
     8341 ccgtaatgac agactgctta aaataattca cgacagcgta aagatagttc ttatcccggc
     8401 ggttaatccg tatgctctga aatcaaattc cacctacaac aagaacggaa taaatctcgc
     8461 ttacaatttc ccgtatcttt gggaacaatg cgaccgctac aaaaagggcg acgcggcggc
     8521 tgaccagaaa gagacacaaa cggttatgag ctgtcttgag tcaataaaaa atgacaaact
     8581 cagtgcggtc atcgaactgc atacaagtaa tattacatat gccggacgaa gcgtttttta
     8641 tacaagacag cacgcaaact gttcaacggc acttgccgat atggtgaaca atttcaacta
     8701 taattataac tatgtcgatt ataccgatga agcggttctt gccgcatcaa acaatgccta
     8761 tctttccgac tatgccgcag atacatacgg tgttaatgcg tgtcagctca tctggtcaac
     8821 gaatctttac ggcggagctt ttacaaacta ttgcataaca aaatatacgg agcttatagg
     8881 aaatacaata agagttatgg cggaaaacag ccgatttatt ccaaagcgaa ggccgcagca
     8941 gtttatcaaa catctttcgt ggagaaaaag cagcgacagc gatgtgttca ctgtaaaatc
     9001 aacctccgag cttgaaaaaa tgccgatttc ctcatacaag cttaagcttg acgccccata
     9061 taatattacg gttaacggct acgttgagct tgatgtctcc gaagcgtgta cggttaaaat
     9121 caatcctgtt ctttatcagc aatattcctc cgagttggat ttcaaaacga ggaaggaagc
     9181 aacgcagttt acgcaggagc ttgccctgac ggcagggacg catattgtgc ctgtctccag
     9241 cataacacag ggatttttca gttgctataa tttcagcaat gataatcaat acactgagga
     9301 attatatttt acgctcatgt tcagcgcttc ggcttcggac aaggtcaagg tgacgtccta
     9361 tgccgttacg cttacggcga ctccgagcga tgttgcaaaa ccgattgaag tcctttcacc
     9421 gatcggactt tgctcggatt attcggcaga tgatattcct acccagaagg ttatttatcc
     9481 gctcgggaca ttatcggaat atgataaaaa ctttaacaac tgaggtgagg gcattggctg
     9541 aaattacggt ttctttgatt gcatttgccg gaacgcttgt cggttctctc ggcggaattc
     9601 ttgccgcaaa caagctgtcg tcatatcgta ttgaacagct tgaaaagaag gttgcaaagc
     9661 acaacaatct tgttgaaaga atgtatgctg ttgaaagctc cgtcaagtcg gcacaccaca
     9721 ggattgacga cctcagaaat gaaatagaat aaaaaggggg actgccgctt gtttttgaag
     9781 tgacagtccc gtatttttta tttaagtccg aagtattcgg aggcgttgtt gtatgaaata
     9841 tccctgatga tatcaataag cgacttgtcg ttggggtaaa gtccctgttc aacccaatcg
     9901 ccgataagtc cgcagagaat tcggcggaaa tattcgtgac gcgggtagga aaggaagctt
     9961 ctcgaatccg taaccatgcc gacgaatttt ccgagcacgc cgaggttcgc aaatgttttt
    10021 agctgttctc tcatgccgtc aatgttatcg ttgaaccacc atgccgagcc gagctgaatt
    10081 tttgaagcgg cttcgcttga ctggaagttg ccgagcattg agccgaggac ataattcgcc
    10141 gtcgggttga gcgcaaagag aatcgttttc ggcagacagc cttcctcgtc aagagaatcc
    10201 aacattcttg aaagcttata tgcgatatcg ccgtcaccta ttgagtcgaa gccggtgtcg
    10261 ggaccgattg acttgaacat tcttgtattg ttgtttctca ttgcgccgat atgaatttcg
    10321 caagcccagc cgtggcgtgc atattcacgc gcgaaaaatc tgaacagctc ggttctgtat
    10381 ttgtcctgct cgtcagcact cgggattttg ccgttgacag cttttttgaa tattgcttca
    10441 agctcatcgg agcttgctct gctgtacggg acatagccga aggaatggtc gctcgcaagg
    10501 cagccgtttt cggcaaagaa atcaattcgt tctttcagaa cctccgtcag cttttggtag
    10561 ctgtcgattt tctgaccggc gactttttcg agcgcctcaa gccacggaag aaatgaaggc
    10621 tgctcaatat tcaatgcctt atcgggtcgg aatgcgggat agaccttgca tttgaagctc
    10681 ttatcctcgg caaggagctt gtgatattca agcgtatccg cagcatcgtc tgttgtgcaa
    10741 agacaggtga cgtttgaatt aacaataagc tccctcggag tgaagccgcc tgcggcaatc
    10801 tttgcattgg ctttgttcca gatttcatcg caggttttct cattaagagg ttcgtaaata
    10861 tcaaaatatc tttgcaattc aaggtgcgtc cagtggtaaa ggggattacc tatgcaggca
    10921 ggtaaaacct tagcccatgc tttgaatttt tcatagcccg aagcgttccc cgttatgtat
    10981 ctttcgtcta tgccgaagct tcgcatggct cgccatttgt agtgatctcc gccgagccaa
    11041 agctcagcaa tatcggcggg ctgtttgttt tcataaatct ccttgggtga gagatgacag
    11101 tgccagtcga aaatcggcat atccgctgca ccctcaaaga aaagcttttt tgccgtgtta
    11161 ttgtaaagaa gaaaatctgt atccataaat ctctccattt tgttcagctc ctttttaatg
    11221 attatacaat atcagaaagc aaaataaaag tgcaaaacgt caaataatct tgaaaaaaag
    11281 aattgaatgt gttaatatat ataaagatca gagtaggtgg tattattatg tcaaaagaaa
    11341 aaggattttt tgcggaattt aaaaaattta taatgcgcgg caacgttatt gatcttgcag
    11401 tcggcgttat tatcggcggt gcttttcagg cgatagtcag ctcactcgtt gaggatatcg
    11461 ttaccccgtt gctcggaatt attttaggta aggtcgattt ttcaaatctt gccgtgacga
    11521 tcggcgacgc ttcaatcggc tacggaaagt ttatcactgc ggttatcaat ttcttcatta
    11581 tggcgcttgt tatttttgcc attattaagg caattaacga tgtttccgac cgcttacata
    11641 aaaagacgga tgaggagcct gctccggcaa caaaaatttg cccttattgc aagtcagaaa
    11701 tcgacattga ggctgttaag tgccctcatt gcacatcgga tgttgaataa ttaaggagcg
    11761 gataatatgc aggaggtaat cctgaaaaag gtgatgttcg gaggatatga ttgctatgag
    11821 gttatgaacc gcataaatgc aatgcagatc aagctgaaca gaacaaaaaa agactcggat
    11881 aagctcgagg cactgatacg ggaagcggac gggctgaaat ccgaaatcgc cgaaaaggac
    11941 gaggaaatta tacacttgac cgagaaaatc agagaacgtg aggaagcgct tgagaaaaat
    12001 cagccttcaa aacttttcct caaaaaggcc gaggaataca cggataatta tattgaggcg
    12061 gcacgttcgc ttgaaaaatc cgtcagagaa cttacggcgg atcaggtgct tgaagccaag
    12121 cagagaataa aggatctgca ggacgagctt gaaagcattt cttcggcaat cggggaaacc
    12181 tttgtatctg tttccgacct taaaaacgaa tataaaaata tcaac
//
LOCUS       DAWBSW010000042        12188 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1132_length_12188_cov_2.704360, whole genome shotgun sequence.
ACCESSION   DAWBSW010000042 DAWBSW010000000
VERSION     DAWBSW010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_12188_cov_2.704360"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    379..388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    714..723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4514..4523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7064..7073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10109..10118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10988..10997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3362 a         2790 c         2764 g         3212 t
ORIGIN      
        1 aatgtaacgt tgttgaatgg agcgctcaaa taagcatttc cacaaacatt gacggcaaat
       61 ccgccgactc aactcttttc tataatataa ctgttgaaaa caacagcttt gataatttct
      121 atggcaggct tgttgatgcg tcaaacgtat caaatctcaa aatttgcagc aacagggtaa
      181 ataataagga cggcagctat gaagccagac gtggaagaat cataatccgc caaagctgct
      241 caagggtaac gatcagcaac aatgaataca aggcttcggt taaagcaccg ttccagagac
      301 tgattgagtt taagaacatt aagtcagcga tataaaagag ctgtgtctga acagttgaat
      361 atttaaattg ttgagtacnn nnnnnnnnct attatagtta atttggaatt atatagagat
      421 tgaaaaatta cgtgggctcc tcctttgggg gagctgtcac gaagtgactg agggagtgaa
      481 aaaatgattg actacaaccg taaactaatt aaaaaagctc aaagtctaag acgcaatgca
      541 accccgcagg aaaataagct ttggtatgaa tttttacgca attatccaat acgatttcaa
      601 cggcaaaaga caatagataa ttatatcgta gatttctatt gtcacaaagc aaagcttgtt
      661 attgaaattg acggttctca gcattacaat gaagcaaaag ctttacttga taannnnnnn
      721 nnntgtcaca gcctttcttt ttacagctta tttctgataa tcttgccgct cgttgttttg
      781 ggaagctcgt ccttgaaaac gacgattctc ggatatttat acggtgcggt gtgctcctta
      841 acataggttt ggatttcctt tttgagctcc tcggtcggct ccgtgccttt agtcaggaca
      901 acggaagcct taacgacctg acctcttacc tcgtcgggag cggccgaaac gccgcactca
      961 agaacatacg gaagctccat gataacgttc tcaatctcga acggtcctat acggtagccc
     1021 gacgatttga taacatcgtc gatacgtccg acataccagt agaagccgtc ctcatctcgc
     1081 catgcaacgt cgcccgtatg gtaaagtccg tcatgccagc actcggaagt cttttcttcg
     1141 tcgccgtagt agccgataaa gagtccgcag ggaaccttgt catgctctgc cttgataaca
     1201 atctcaccct cctgacctac gccgacgctc ttgccgtcgg ggtcaactat atcgacatca
     1261 aatgtcggaa cgggcttgcc cattgagccg agcttgtgtt cggagccgaa gaggttgccg
     1321 acaacgagcg ttgtttcgct ctgaccgaag ccctccataa ccttgagacc ggtaagcctg
     1381 ttgagctggc ggaaaacctc ggggttgagt gcctcgcctg caatcgtagc atgctcgatc
     1441 gagctgaggt cgtactttgt gagatcctct ttgatcatca ttctgtacat cgtcggcggc
     1501 gcgcagaagg tggttatatt atatttcttg aacataggaa gaatgtcgga ggcgtggaag
     1561 cggtcaaagt cataaacaaa gatacctgcc tcgcagagcc actgaccgta aagcttgccc
     1621 caaagagcct ttgcccaacc tgtgtcggaa atcgtcaggt gaagtccttc cggatttacg
     1681 cagtgccaat agtttgccgt tacgaaatgg ccgagaggat atttaaagtt gtgggttgcg
     1741 attttcggat atcctgttgt gcccgaagtg aaaaacataa gcatcttatc gttaccgcat
     1801 gcgctttcct cggtacgttc aaactccttg ctgaaaagct taacctcatc attgaagtcg
     1861 tgccagccct ccctcggctc gcctacgata atcttcgttt tgagcgtcgg tgagtttttc
     1921 gccgcaaggt caacctgatg cgccgtatcg tcgtccgagg tacagagaat tgcgcttacc
     1981 cctgccgcat tgaaacggta atcaaggtca tgctcctgga gaagatgggt tgccggaatt
     2041 gcgattgcac ctatcttgtg gagcgcaaga atggagaacc agaactgata atgacgcttg
     2101 agaacgatca taaccctgtc gcccttcttg atgccgagcg acttgaaata attcgccgtc
     2161 tgagctgaat actccatcat gtccttgaag gagaatcggc gctcattctt attcttatca
     2221 atatgaagca ttgcgagctt gtccgggagc ttctttccga gtccgtcaac aatatcaaag
     2281 gcaaaattga acttatcctc gttctcatac gaaagcgaaa gaatcttgcc gttttcatcc
     2341 tccttaggat gaataaagtt gtggtaaatt cgcttacctg tgccggatct ctttggtgat
     2401 tcgtcgtcaa tcgtgttctt aggcgtaacc acctcgcctg ccggattaaa aacaagtgca
     2461 tagaacgtcg tttcttcctc gtcaacggca atcattccgt gaggcgttga gctgtcaaaa
     2521 tagatcgtgt cgcccgggtg gagaatttca acatggttgc cgatctgcac tctcagggtt
     2581 ccgctgacaa cgatatcaag ctcctgaccg gcatgagttg tgagggcaat atcatcgctg
     2641 ccctcattag gatcgcatct gacgaaaagc ggttcacaaa tcttattttt aaatgctgcc
     2701 gcaagattat aatattccat gccgtgggcg tggtcaatct caagaccctc gccgctcctt
     2761 gtgactgtgt acgatgcgag aacggggctt ttaccctcga taatatcatt gacatcaaca
     2821 ttgaaagcaa ttgcgcacct gtacaagaac gcaaacgtga gatccttctc gccgcgttcg
     2881 caggcaatat aatcctcgat agtcatgtcg gtcttttccg ccatttcggc aggagtaaat
     2941 cccacgatct cacgaagctc cttgattcgc cgagccattt cgttgatctt gaaattcaag
     3001 cctgtcattt ctttcatttg tgaccattcc tttctttatt ttatagattt taagacaata
     3061 aaagtatgtt tagtattgat taaaactttg ggctgtataa ctgaaaaaga aacctaaaat
     3121 cggtttgtga gaaaattgtt cacagtggat tttgcagttt gggaactgta cataggtact
     3181 gcatgcgctg ttgcaatttt ttccgattac atcttgacga gagatttttt cgttagacag
     3241 ttcaaaggtc tcgaaggcta tattcttata tgccgaggga gctttgggat gtataacgga
     3301 aaaagatccg tcaagaaaaa gaaaaacccg tctcgaggct tctttgcctt gagacgagtt
     3361 gtaattattg atttactacc cgcggtacca ctcaaattgc gttcattgaa caccacttcg
     3421 gactccaaca agtcctatgc tttcacgcag ccttacggga gggttctact tgaaaatctt
     3481 tcttccctcc gactcagaag ctacaaacct aaaactcacc tatcggcttt cacctgccgc
     3541 cgactctctg gaaagctacc gctttaagtc ctcttcgtca ttgtctttaa atattttatt
     3601 tgtacgaatt ttaacacgac ttttttatct tgtcaagcct ttgaaaagaa tttttttaca
     3661 atttgtctta tttatgatat ttcccgccgc tgtttatgcg aaaagctctg taaatctgct
     3721 caagaagcat gattcttgca agctgatgcg ggaacgtcat ttcagacatt gaaagcctga
     3781 aatcggaacg ctttttcacc tcgtcggaaa gcccgtatga gctgccgatg ataaaaacga
     3841 cattgctgaa accgagtacg ccgcaggaat tgatctccct tgcaagcctt tcggagctga
     3901 gctgtttgcc ctcaatacac atcgaaaaga cctttgctcc gctcggaatc ttagatagta
     3961 tctccttgcc ctcggcattc aaagcatttg caatctcgct atccgacgga ttctcgggga
     4021 gctttttcgg cgaaagctca attatattaa gcctgcaata tgctccgaga cgtttaacat
     4081 attcgtccga agcgtcccga agatactttt ctttgagctt cgcttcgcag attatcgtaa
     4141 cgcctaacat cacagcacca ccgtttccgg ttcgtctgag cctgcgacct tgagtatgta
     4201 atccctgttt tcggtcgctc ccatgcagtc aagtgccgat tttgaagttc tgtatgccaa
     4261 tgacggaata ttgttttctt tgctcagatg tccgagaatg agacgtgttg taccgctttc
     4321 tacaagctcg gtgacaaaat cgccgcacgc aatattcgaa agatgcccga catcggaaag
     4381 aatacgtcgc tttaaaagat acggataact gccgttgcgc agcataccta tatcgtgatt
     4441 gctctcgaca agtgcaagat cgcagcccgt gagcgccgtc ctcatttcgt cggtaacgat
     4501 tcccgtatcg gtcnnnnnnn nnngattttt ctgctgtcgc tcgttgtgac ggaatagccg
     4561 cagctttcgg caacatcatg cgaggtacgg aacggaacga caagctggcc gttacactcg
     4621 acaccgtcac cgataggaat aacaggaaat ttgccgttta taatgcccat tttctcaagc
     4681 tctcgaatcg ttccctcagt agcatatacg ggaatattgt acttcgacgc caaaacacgc
     4741 agacccttta catggtcacc gtgctcatgg gtaacaaaaa cagcaccgat attcctctcg
     4801 ctgacaccta tgccgttgag cttcagtgaa atctgctttg cgctcactcc ggcatcaatc
     4861 agcacaccgc cgcccgaaga gcctatatat gtgcagtttc cgctgcttga cgaaaagagt
     4921 gaacaaaaac gagccatatg taccttcctt atatataaat aaaaatactg ccccgagctg
     4981 ctcggggcat aaagaattca ataagacatt ttgtgcgagt ctgtctcaac ctacctgcgt
     5041 atcctccaac ggcttaggct tgtcctcctc gcttactcgc acaatatctg cgccgaggtt
     5101 gcggaacttg ccgacaacgt tatcatagcc acggtcgata taaactatat tttcaattga
     5161 ggtcgtgccc cttgcaataa ggcctgcgat aagcatcgcc gcacctgctc tgaggtcatc
     5221 tgccttgacg ggagaaccgt tgagatattc aacgccctct atagtagccg ccttgccgtt
     5281 gacggaaatg tttgcgccca ttcttgccag ttgctcaaca tactggaaac ggctgtccca
     5341 aacaccctcg gaaacaatgc ttgtaccgtc ggcaatcgag agcatggtag caaactgcgg
     5401 ctgcatatcc gtcgggaagc ccgggtgagg catcgtcttg atattgcatt tacccggtct
     5461 gccgttgccg attacacgaa ccgaatcatc gtattcctca accgttgcgc cgcactggat
     5521 aagcttggct gtgattgact caaggtgctt cggaattacg ttcttaacaa gcacattgcc
     5581 gttagttgct actgcggcaa ccatgaatgt gccggcttcg atttgatcgg gaattatcga
     5641 ataggtgcag ccgtgcatat gctcaacgcc gcggatctta ataacatccg tacctgcgcc
     5701 tctgacatcc gcacccatag agttcaaaaa gtttgcaagg tcaacaatat gcggctcctt
     5761 agcggcattt tcgataatcg tcaagccctt tgccttaacg gcggcgagca tgagattgat
     5821 agttgcaccg acggaaacca catcgaaata gacaagaccg ccggtaaggt ccggagcctc
     5881 aacatcaatc atcgcattct ccttgagagt tactgttgag ccgaggatct caaagccctt
     5941 gatatgctgg tcaatcggac gaactccgaa attacagccg ccgggcatag cgacctttgc
     6001 cctgccgaag cgggcaagca aagagccgat gagataatac gaagctctca gatgctttgt
     6061 catatcgtgg gaaacaacgt atgaattgat tgttgatgag tctatatcgt aggtatttgc
     6121 gtcaacctta gtaatttttg cgcccaactg atgaagaatc cttatcatca tcgtaacatc
     6181 gctgatatcg gggatattct caaccctgca cacgtcctgt gcaaggataa ctgccggaag
     6241 aattgcgaca gcggcatttt tggctccgct gacggtaatc tcgccgtgga gcggctttcc
     6301 gccgttgata tatatcttat ccacccgtta cactccttaa aaatttatat atgtaaaaaa
     6361 agatgaaatt ctcatttatg ccacaaaagg ggcaattaac caaatatatt attaaacttt
     6421 taagattata ccactaaatg aaacaaagtt caatattttt ttattttccg caataattca
     6481 tattttatgt ctcaaaccgc tatatataga atatattatc cgcttatata actatatatt
     6541 gtgttatatt gagtaaaatt aaatataagc caacattttt tcagcatatt gtacaaaaac
     6601 ttattttcat tttttctata tacaatatgt agatatgttc ggttgaaaaa atatcactgc
     6661 cgttaaggca atgccgagca attaagctgt tgcattgaat tcttgtcacg gttattatgc
     6721 gttaaaccgt caataataat gtaggtgata ttatggaaat ttttaaaatt tgcttaacaa
     6781 ccgttttgtc aattatcgag ttgtttttcc tgacaaaaat aatgggcaag cgtcaggtat
     6841 cccagctcag ccttttcgac tacatcaacg gaataacgat cggctcaatt gccgccgata
     6901 tggcgttcag tccgcttaac gaatgctgga agcctgccgt tgccattgtt atttacggta
     6961 tttttgcggt gcttttttcg gtgcttgcga acaaatcaat taaattccgc agattttttg
     7021 tcggcaaggc ggttattctc atggataacg ggacccttta ccgnnnnnnn nnntgagaat
     7081 ttcaagaaat cgaagcttga tttgaacgaa tttctgacgc agtgcagaat aaacggttat
     7141 ttcaacattg acgacctgca aacggtcgtc atggagccga acggccagct gagctttctt
     7201 ccgaaatcga cattaaggcc aataaatccg caggatatgg gcatacatcc cgaaaaggag
     7261 acttctccga tcgttgtggt ttccgacggt gaaattctgt ttgagaacct caaggcctgc
     7321 ggcaagaatg aggtttggct cagaaaagcg ttgaaggacg gcaattttcc tccggtaggc
     7381 aagattttta ccgcctttgt caatgacggc aagctttccg tctacgagat aaacgaagaa
     7441 aataacgaaa acgatatata cagttaaaaa tcatttcacg gcctcctgcc aaaattacgg
     7501 catgaggtcg tttattttgg ctttaacact tgttttattt tcgccgatgt tgtaaaatgt
     7561 ttgtatcaaa tttcggagag tattatgaca gtattgagtt ttcaaaatct tgcgttctct
     7621 ttcggtgagc gtgagctttt cagaaatgtt cattttgaaa taaacgataa agaaaaggtc
     7681 ggctttatcg gctctaacgg cgtgggcaaa accactatat tgaagctcat cagaggagag
     7741 cttgaaccga cggagggcgc aatcgtaaca ggcaaggagg caaggctcgg ctatatggag
     7801 cagcacacct gctcaaagaa gggaataacc ctttatgacg agcttatcag cgtttttgac
     7861 gacctgatga accttgaaat tgagataaat caggtcaatt accgccttga gcataacctc
     7921 ggcgatatgg aggatgatct gcggcttcag gacgagctga cgactaaatt caataacggg
     7981 ggcggtctga cattcagaag ccgcacaaga agtgcgcttc tcggcctcgg attcacagag
     8041 gacgatttta atttaacgac agacaagctg tcgggcggtc agcgttcaaa ggtcgctctt
     8101 gcaaagctcc ttctttcaaa gagcaatctc ctacttcttg atgagccgac taaccacctt
     8161 gacataaagt cggtagaatg gcttgagaac ttcttgaaaa attacaacgg cgcaatattt
     8221 gtaatatcgc atgaccgtta ttttctcgat aaaataacgg acaaaaccgt ggaacttgaa
     8281 aatcgcaagg tgcgatgctt caagggtaat tattccgaat tcctggtcaa aaagaaggca
     8341 gagcaaaagg cgattgagga taaatacgaa acggatatga aagagataca gcggctcgaa
     8401 ggcgttgtgg ctcagcaaag gcaatggaac agggagaaga acatcaagac tgccgagagc
     8461 aagctcaagc agattgagag aatcagagaa cagcttgttg ttcccgacag caaggttgag
     8521 cgaatccgat ttcgctttga gccgaaatgc gtcagcggca acgacgttat tatcgccgac
     8581 gggctggcta aaaggttcgg caataagaca gtattccgca acgcttcgct gcacataacg
     8641 aggggcgaaa gagtgttcat tctcggtgac aacggctgcg gcaagacaac atttctcaaa
     8701 acgctgatgg ggcagtattc tccggacgag ggcacattca tattcggcga aaatcttttc
     8761 aagggctatt tcgaccaggt tcaggcagga ctcgatctca ataaaacagc gattgaggag
     8821 atttggtcac attatccgaa aatgacgcag actcaggttc gcagtgcatt ggcggcgttc
     8881 cttttcaaag gggacgatgt tttcaaaaag atttccgagc tgagcggcgg agaaagagca
     8941 aggatcgcac ttctcaatct tatgctcggc ggttacaatc ttctgctttt ggacgagccg
     9001 acgaaccacc ttgacgcatt ctcccgcgag gagcttgaaa acaccctgct tgactatgac
     9061 ggcacgatgc tgatagtttc ccatgaccga tattttataa acaagctcag ctcacgaatc
     9121 ctcgagctta atgagaacgg atttacggaa tacctcggca attacgatta ctatattgaa
     9181 aagaaagctg cacgtcagcc taccgcagta aatactgaca cggcgaaaaa agccgaaaaa
     9241 gtcaacgact acaagctgaa aaaggagcag gcatcgcagc agcgaaagct caaaactcag
     9301 ctgacaagaa cagaaaatga tattgcatcg cttgaaaaag aaattgagga tattcaggaa
     9361 aaactgaata cggaagaaat tcagaccgac tatgaaaagc tgctcgaatt aacagacctg
     9421 ctcaatttaa agaacgatga attgcttgac aaatattccc tctgggacga actgcaggca
     9481 atggttgagg aataaaacga tcatcaacaa aacgaaagga caatatatgc cggaatataa
     9541 agattatttc ttggagcttg aggacgttga aaagcacaat tcctttatcg gccgcaagcc
     9601 gaacatggcg gatctgcctg actttgagag caacaaaagt aagctccccg agcctgtctg
     9661 ggacggtcac gccgattcaa tcgaagccta ctataaggcg tggaaaatcg cattctcaaa
     9721 tctcggcaag ccgaccgagg agaacggctt tgtttccccg tacattgatg ccgcattcaa
     9781 cggcgacatc tttctttggg acagttgctt catgctgatg ttcggcaaat acggcgacag
     9841 cgtgttcaaa ttccaaggca cgctcgacga tttctattgc aagcaggagc cggacggatt
     9901 catcggccga cagtaccatg aaacaaacgg cttttcaaaa tttcaccgcc ttgaccccgt
     9961 aagcacaggt ccggaaattc ttgcatggtg cgagtggcag tactatcaga actacggcga
    10021 caaaaagcgc cttgccgatg tttattatcc gcttctctgc taccacagat ggatgcgcaa
    10081 ttatcaccgc tggcaggacg gttcctatnn nnnnnnnngg gctgcggaat ggacaatcag
    10141 ccgagaaccg accttgaagc cgttcccgga gttgaggatt ggcaggttga aacattccac
    10201 cacggcttta tgtcctggat cgacgcaaac ttccaggctc ttctaagctg cagggagctt
    10261 ctgaaaatgg caagggagct tgacataaca gacggcgttg acgagcttca aaaagaggct
    10321 gaatacctga cgaaattcat caacgaaaaa atgtggtcgg aggaagataa atactatttt
    10381 gaccgcagag gcaacggtga gctgctcaag gtcaagagta ttgcaagcta ctggggtttg
    10441 ctcgccgacg gtgtacccga ggagagaaaa gccgatttca tagctcatct tgaaaatgaa
    10501 aaggaattca agcgtccgca ccgtgtgccc gctctttcgg cagatcaccc cgattattcg
    10561 gacgacggcg catactggaa cggcggcgtt tgggcaccca ccaattacat ggtaataagg
    10621 ggtctttctg agtgcggcag ggaaaagctc gctcacggaa tcgccctcaa ccactacgaa
    10681 aacatgatgg aggtttatcg caaaacgggc acattctttg aaaactatgc ccccgaatcg
    10741 gctaaccccg gcaacccggc gaagggcgat ttcgtcggtt gggcaggaat cattccgata
    10801 accgttctta ttgaatatgt tctcggaatt caggttcatg ccgagaaaga tgaaataata
    10861 tggtatgtaa acaatcttga gcgccacgga ataaagcata ttcccgtcgg cagagacgca
    10921 tacgctgacc tcatttgcgg agcaagaagc gatgcaaatg agaaaccgaa tataacagtt
    10981 aagtcagnnn nnnnnnncag ttatttacgg cgacaatgaa tttgtgattg gagaatgatt
    11041 atgaagctcg gatttactct ttacaatttt cattcggtaa tagactctct tgaggatctc
    11101 gataatgtgc ttgcgaagct tgagaatatg ggcgttgata ccgttcaggt atcgggcata
    11161 ggctacctca ataactatga cgttgcgaag ctttgcaaaa agcacggcat ggaggtttgc
    11221 ttaacccacc tttcgttcga cagaatcgtt aacgataccg atgcggtaat tgaggagcac
    11281 aaggctctcg gctgcaagaa tgtcggaatc ggctggatcg aagaaaaata ccgcggcgag
    11341 gacggcgtca agaagttcgt tgaggagctt acccctgctg tcaaaaagct tcatgcggca
    11401 ggaatgacct tctcttatca taaccatcag tttgaattta tgagatatcc caacggtaaa
    11461 acgggatatc agatgctcac ggagcttgat cccgaactga taagtttcat ttttgacata
    11521 cattgggcac agaccggcgg agtcgacccg gcatcatata tcagaaagct cggcaagcgt
    11581 atcagcgtgt gtcacttcaa ggattatcgc attattaagg atgccgaggg caattttgtc
    11641 cgtgattttg ccgagatcgg aacgggaaat cttgaccttg acgagtgcta cagagcgtgc
    11701 cgcgattcgg ggatcgaata tataattata gaacaggatt cgactccgct tgatgttttt
    11761 gactcggcag aaatcagttg gaagaatctc aaaaaaatcg ctgaaagaaa ctgaaaaaac
    11821 gaacctcctg ccaagtgaca ggaggtaaat ttttgttatt tcttataatc tctgagaatc
    11881 ttgagcgtag tcgttttcgg gaattcgttg tctctcgtct gaattcttgt tacccgtttg
    11941 taagagggca tctttctgtt aatctcatta acgtctgcct tgattctgcc ctttgcgtcc
    12001 ggtgcttcaa cggtgtcaag gaaaaactcg gctgttatat agcccttttc ctcatataca
    12061 acaacttcct taacatagtc gattgtcgaa agcttgtcct caatctcctc gggagaaacg
    12121 ttcttgccgt ttgaaaggat aataaggttc ttctttctgc ccttgaagaa gaggaatccg
    12181 tcctcatc
//
LOCUS       DAWBSW010000043        12107 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1144_length_12107_cov_2.789413, whole genome shotgun sequence.
ACCESSION   DAWBSW010000043 DAWBSW010000000
VERSION     DAWBSW010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_12107_cov_2.789413"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2180..2189
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5407..5416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6223..6237
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3281 a         2860 c         2505 g         3426 t
ORIGIN      
        1 gtcgttacgg ggttggaagc cgatagaaag gagctttccg ctctccttct cagccttgca
       61 gatttcaacc gcttcctctg tcgtaacgca catcggcttt tcaagaagaa cgtttacgcc
      121 gtgcttgagt gaataaatag tacactcggc gtgcgtcatg ttgtatgtgc aaacgcttac
      181 ggcatcaagc tcaggctcgt tgtcgataag ctcctcgtga ttcttgtaaa aatgtacatt
      241 gtcaaggcgt ccatccttgt tataccttgc ggcaaacttc tcagccttgc ccggaatgag
      301 gtcagccatg gcaacaatgt caacatcgtc catgtcgaga tagctctcaa tgtgtgcctc
      361 tgcgatccag cctgtgccga taatgcctac cttgaatctt ctgcccgttg agaccttctc
      421 ctcctcaacg gcctgggtga attggttcaa tactgcgtca gacatattta atctccttta
      481 tttgttaatt tctttaatgt aatcaatact catttttgcg cactcaagcg gagtcttgtc
      541 aagagcgggc tgatcctgct caacaacaac ccacgatgtg ccggctttct tgcaagcttc
      601 aagaatttca gggaaattct gaacgccgta gccgcaggga cggaagccaa atgcttcttc
      661 ctcctcggcg tcctgcgcac cgtcgtcaat gccgataagc tgatagagct ttgccggctt
      721 gcccttgccg ctcatgtgga aatccttgag gtgaacaacg ggtgctctgc ccgaatactt
      781 ttcaacatac tttgccggat cctcgccgcc gacattgacc caacaggtgt caagctcggt
      841 ctgaagctca tccggagtaa gcgttgaata gagaagatcg agtccgtaaa catctccaac
      901 ctttttaaac tcgaagtcgt gattatgata aaggaatgtg ataccgtact ttttacctat
      961 agcggcaaag cgcttgattc cctcaacagt atcgtcaaaa agctctgcgc cgggacgtct
     1021 ttcctcaacg gcatacggaa ttgcgatata cttgcaaccg atctcggcat aagttacaat
     1081 tgtcttttcg ggatctgcat caagctcgtc atacggaacg tgagcggaga tcggctcaac
     1141 gcctacctcg gcgcaaatct ttttaacctc ttctgccgaa tgatcaaaaa gtcctgcgaa
     1201 ctcgactccg tcatagccca tgtccttaac cttttttatt gtacctgcaa agtcctccgc
     1261 catagcgtcg cggacggagt aaagctgcaa agcaacagga aaactcatag taaaacctcc
     1321 taagatgtgt tttgtttatt ctatcacata gcaaaaaata ttacaataaa ttttgataaa
     1381 tttatttaaa aacatacaaa agacagatta taatcgctcc gtaaagtgcg ccgagaagca
     1441 tgagccactt gtctttaaga ataacgctga tgggatcgcc gtcggaatcg ccctcaacgg
     1501 tcagactgta cttcatacaa atgataataa caagcggaat cgtgaacgca atcttattgg
     1561 aataaatcgc aatcgttccc gaattgacgc accaaagaga atagaacact atgcccaatc
     1621 ccagcgtcat atacatgttt ttatcgagga aatccttgtt ataatatttc agcaccttcc
     1681 ttgtattctt ctcgctgttt atgatttcac ctcggcgctt tccgagaccc atatagaatg
     1741 aaaacgccat taccgtaaga taaagccagc ttgaaaccgg aatttcggtg atcacggaac
     1801 cgtaaaggat acggataagg tatcccgaaa caagaatcag aatatccaga agcacaacat
     1861 ttttcagtcc gaagctgtag ccgagattga gcacaagata cagtgcgatc aacagccagc
     1921 cgtaaagatt tttttcgcag acaaaccagt ttatcgcaac ggaatcgaca agtaaaaagc
     1981 acgcaagcac agccgcactt ttcttttgaa ttttccccga agcaataggt cttgtacatt
     2041 tggtcgggtg gtgtctgtcc ttttcggcat cacaaatatc attgattata taaacaacgg
     2101 agctgacaaa gctgaacacg acaaaaccgc cgataacgtg caaaagcctg tccttctgaa
     2161 gcaggcttcc gccgaaaatn nnnnnnnnnc tttggcctta tgagcctgat atattcttta
     2221 agcttcattt ttatgctcct tgagggttgc gactgactga ataagaattg ccgccgccat
     2281 tataatatac ggtaaaatcg ggaaatgata cctcggtcct ccgaccaaaa gcatcgttac
     2341 tccgattgca ccggctacgg taaatataaa cggcataagc ttcgccgctc tgcgcttgac
     2401 gatcaggaac acaacacatg caaacgcaag aacaagcgtt atcatgtaaa ctgcctgcgt
     2461 ccagataacg attatatccg ccgacgagag attctcctcg gattgacccg tcattctgcc
     2521 gataagtcct ctgtagaacg caccgccgtt gcctgccgtt gcatcgttat agaaggtgcc
     2581 gagcgaatat ccctcggaat agagcgtgac aagcattttt ttcggcatga gcttaatata
     2641 atcgcccggg ttttccttta tccatttcat cgcttcggat ttataaaaag cgtccttctc
     2701 cttgaaggtc atttcgcttt tctgtttctc ggaaagatag cctgctccgc cctcgccgaa
     2761 aacctcaaac ttcgatgttc cgtctgcgcc ctccgaagcg cccataagga gattgtagcc
     2821 gcccgtcgtt gcctggtatg caaaatatcc gcaattgaag taagctatgc cgccgatgac
     2881 acaaaccgtt accgctgcgc cgagcacaag gcaaagatat tgtttcagct tggctctctg
     2941 cgttgcaaga agccaaattc ccgctatcag aaaaaccaca ccgagcggcc ttgtccagtt
     3001 tgcaagagca aaaaagattc ccgaaatcag gcaagtgtat attttccccg tcatcagcaa
     3061 tgccgttgcg ccgaaggcaa aagccataaa aatcagctcc gtcctcgcct gtgcgacctc
     3121 accccaaaat gtgcccgtag agcagaaaat aaccgtgaac caatattgaa tttccgtatc
     3181 accgaatatt tttctgatta tatatatgca agaaatcaaa atcgcataaa tgcaaaaaat
     3241 attgacaaat tttatcgctt caattgatcc cgtaagcctt atcgaaagta tcaaaagatt
     3301 gataagaccg ttgccgaaga tatatgtgtc gtgaatattg agcgatgacg gataccaaac
     3361 gccgttcttt accgcattag tcgcaagctc aaaatatcgc tgagagtcag aagcaagcac
     3421 gaccttatga gtcagcaaaa cataaatctg tattccgagc attacggcca tcgcgataat
     3481 gctcagaatt ttcaaaaccg atttatattt cctgccgccc gacgaaaaaa tattcagcgg
     3541 ttcggcagct ctgacatcaa atatttgtct gaagaacata cactcaccca atcatagcat
     3601 ttaaggcttg cgtcgcaatt tgtgcggcat tgacttattt ttcctctttt ttcttatgga
     3661 aaagaatttt gtcaaccggg aaatagatga agaactgtat tgccgagcaa gccatcgttg
     3721 aaatatttgc ggcaagctga gcagacaggc cgaatgaacc gatgagccag ccctgaattg
     3781 tcggagcaag ccatgccgag aagcagatga gtgcaaccgt aaaaacaata taaatcggca
     3841 gagtaattgc aatgttgctc gacgagccga aggtcttttc acggtttaca aagaatgcga
     3901 cgatctgtgc aagaatattt gcaatattga aagcaaggaa atatccgaag cccttaccct
     3961 cgccgacata gctgaaaaca aaccatgtga aatcctgatt cattatcgcc tttatctgcg
     4021 gcaaaagcat gataagattc aaagaggcga actgaactat gcaagccaac aggcttacaa
     4081 ctatgaattt aacaaactgc caaagagtaa cgattcccga gcccttctgc tctgcggatt
     4141 tatcaatgtt ttttaatccc ataatacaac ttcctttcat gtaaccggag gttatcaaaa
     4201 aattaccgcc gacagtcggt tccgccgtat gcgtaccttt ttctgtcagc ggtagtttta
     4261 gtataccatc tgttgcgaat tatttcaata gtgcagcgtt atttaacggg tatcataagc
     4321 atcatttttt caccgatttc atcagaggtc aactcgtttt cactcataat atcctctacg
     4381 gtcgtgttat attttcttgc gatgtcccaa atcttttcac cgccgttgca gaaacagata
     4441 acaagcgatg agtgtctctt ttttaactcg gaattttcca ctgtcttgag acttacaagc
     4501 gtgcgacatt cgctcgtctc ggttatatcg gcgctgatat tcatttccgc tttgaattga
     4561 gcccgaccgt cgctagtcag tgtacagcta tagcctgtta agaaaagctt cgggtcacag
     4621 tgcagattgc cgcctttgtc atcaatggac cgtgaatatt caaattcggc ttctctctca
     4681 cagaaggtcg gtgttccctc tccgtcaatg agaataaatt tcaacggaat ttttcccgta
     4741 accgtcattt tgtccgcatc gaagcggctt tttgcttcgg gcgactgtgc aatggcggca
     4801 taaatcctct gcggattcat tgaggaaaga tcgacgcttt cacggcagat aatactgtca
     4861 ttaagatttt gtaaaatctg agtaaatgtc tctgttttat actttgcatc tatctccttt
     4921 tgcgttgaat aagaatcggt tataacacaa atatcctccg tcctatatgc cgtcacaaca
     4981 gcacaaatat cacagccgag attgagatat ttatactcgc cgtcgttgtc ggtcttcggt
     5041 tcggcctgaa cctgatcaac gttaaagccg acacttacct tgcagctatc gtcaatactt
     5101 tcgatatcaa ttatctcgct gaaaggcagg ttataattaa cgcaaacgct ttcgttttca
     5161 cccgttgccg agcaatacat aaccttgaga acggcctcac ccttgagaag tattttacct
     5221 ttaattattt tagtctcgga caaaacagga gaagcacagg cataaaagat tttgccgatc
     5281 tctcctccgt cggcttcttc ggtctgagaa agctgaaatt ttttcataac aacggcagca
     5341 acgctatccg tgccgatttt cttttttctc agctgaattc cgtcaccctc ggcgtctgtg
     5401 ataacgnnnn nnnnnncaca acgccgtgaa tatcaattct ctttttgctg accgcacgac
     5461 agtttgcata ttcggttttt gccgaagagc aaagcacagc accgtcataa gtctgcggaa
     5521 gctcgctgaa ttttgagaaa ggatagtcct gctcatagca ataaattccg tttttgtcgt
     5581 cggcataaac tattcgtatt tttgcgttgc cgtcaacggt ggcgcggtcg ccggatatct
     5641 ttgaatttgt tatgctgttc gtcacgctgc acctgagaat tctcattatg tcggggcagt
     5701 aatccggcaa agaaatatcg caatcaatgc cttgttcggc aaaattatcg taaagtacct
     5761 ccgccgttcc tatgtcggtc tttgtcaatt cgatgctcat ataatcaacc tctctttttt
     5821 tatttcactt gattatatga aatcaaaggg acaaatatga ttgtttatgc aagaaccagt
     5881 gtccagccct tttcggcaag tttgttttca aatatcttcc tttcatcggc agtcggataa
     5941 ttgttttcaa aagcgtatgt tgataaagta ataacgccgt cttgtaagac ggcaagtccg
     6001 tcaaagatgc tctccatact ctcctttgaa agtgacttgc acgatgcaaa ggaaatatcc
     6061 atgccgatgg tttctttttt aaatcttata gtttgcagac ttgtgcactt atcaaatgta
     6121 cccgaaatcc aatttgccgc actcatattt aacgaagcat ttatttcctt gagtgaaata
     6181 cagccgaaga acgtctccga ccagtcctgc attctctcac aannnnnnnn nnnnnnngca
     6241 ttgctgcatt ttgtcaaatc tccgagtatt acttctcgaa gcgaggggga atttgagaaa
     6301 cagtttatca tcgtcgttat ctgatccgtg tttctgagtt caattcttcg cagattcttt
     6361 ttctgggtga acatctgcga aagtgttgtt gccgaggtca aatcaagtcc caattcggta
     6421 acattattgt caaagttgcc agaggacaaa attgccatgt tctttgtgtt taaaaaagga
     6481 agcttgtatt cctttgtgcc gcctgtccac agtgcaaaat tactcctcgt tttgtctgcc
     6541 atagccgcat cgacaagatc ccatttatcc gccttgagct gcgcagcgga aagagattta
     6601 ttgagttcgg caatttttgt gttgaaagta agattttcct cggcgtgctc ttgacgaatt
     6661 ttcgcaatct ccgcgtcctt ttctttttca acattttttg tgttctcgtt tgctttgttg
     6721 atttttactc ccatttccag aattttcttt tcataggcat acttaagcga ctcaatttca
     6781 tcctcgtatt ttttgtatgc ctcgtcaata ctgtcctgtt tgaatctgtc aaagagagtc
     6841 tcgctcggcg aaaacgtctt ataatcgtcc ggcaaaagcg atgaaacaac cctgacatca
     6901 accttgcctg ccgtgagcac catagacccg tcatagccta taaccgagag ttcaaaatcg
     6961 tgatcgacag ccagcacctc ccaaggcacc tttgccgacg attcctttat ctcgataatg
     7021 tagtattcgt tttttgctgt tctgaaaatc gcagcctttg cgtcaaagat tctccagttc
     7081 gcatcaaaat gaaagcgaag tgaaattgtt tcgttcgcat tggcggcaat tgcaatcttc
     7141 tctgcgccga agattcgctg tttttcgatt cttaaatcat aattaaccat ttcgtatctc
     7201 ctctgcttcc ccatagcatc atgtatatta tagcgaacat ttgttcgttt tgtcaatagt
     7261 aaatttttac tttacaatag aaacgcgata atgtataata tttttggaat ttttcattga
     7321 aaagaaggtt taaaaacatg gaaatggtta aattatcccc ctttgttgcc gacaatatct
     7381 ggggaggaac aagactcatc accgaatacg gtatcaagac agataaagac cctgccgccg
     7441 aagcatgggt gctttcatgt catcctgccg gaccgtcaac ggttctcaac ggaaagtatc
     7501 agggtaagac cttgcaggag gtttacctcg ccgacaagag catatgcggc acaaagggaa
     7561 aaaaatatga atttttcccg cttcttatta aattcattga cgcaaagaga gatctttcga
     7621 ttcaggttca tcccgatgac gaatatgcaa tgcgcgttga gggcgaatac ggcaagaccg
     7681 aagcatggta cattcttgat tgtgagcccg acgcagagct tatcctcgga ttcaaccgca
     7741 atgttacggt tgaagaattc aaaaaagccg ctcagggcga cgagatgatg agcatatgca
     7801 acagagtcaa ggtcaagaag ggcgatgttt tcttcatcga gtcgggcaca atgcacgcaa
     7861 tatgcaaggg catacttctc gccgaggttc agcagaactc caacacaacc tacagaattt
     7921 acgattacgg ccgtctcgga aaggacggaa agccccgtga gcttcacgtt gacaaagccg
     7981 ctgatgttac aaagctctgt cccccgtcta ttcccgattc atcgaaacgt gaggttgaaa
     8041 agtttgacaa cgcaagcaga agtaaactca catcatgcga tcttttcaca atgtacgaac
     8101 tgagcgtcga cggggaatat tcatcggttt gcgatgagca gtcgtttgta tcactccttt
     8161 gccttgaggg cagcgccgag attgaatgtg acggcgaaaa gcttatgatg aagaagggag
     8221 aaagcatctt cattcccgca aacaagggca agttcacaat caacggaaat gtaaaaattc
     8281 ttgagacaag attataattt aaggcataaa aaaaagaggt tcagattgaa cctctttttc
     8341 gttatatttt attccgtctc tttgagcttc ggcgtaatgc cgcatttgag cgccttcgga
     8401 agctgagctt ccgcttcctc ccttgtgatc tcgcctgcgt tatatcttgc catgacctga
     8461 acatccttgt ctctgagctt atagaaaaga tagataaacg acgttattgc aagtccgatg
     8521 ctcggaacaa gtatgtacgt tacccagaga ccgttaagcg ccgttgcgct ctgaacaatg
     8581 tgcaattttt cgatatcggc aaagctttcg gcttcgatct ttgtccaatc aaaaaacgta
     8641 agaatcgcaa gaccgagtga agaggcaatt gccgctgcga ttttcgttgt gaacgtttga
     8701 attgagaatg tgattccttt tgcatcaacg cccgttttat actgaccgta ttccgcacaa
     8761 tcgggagtga acataaaccc gagcgtaccc gttacactgg tgaaaattcc tctgacaacc
     8821 gtaagcacaa tgaaaagcat cttgttctgc cagccgatga aatacattgc caatccaagt
     8881 attgccgcaa cggtgttgca gataaaaaac agtttgaatt tgtcgaattt ctttgaaata
     8941 acgggaataa gcagagccgc aataaccgac ggcacaacac cgagaattcc gagaattata
     9001 ttgaagttcg cactgccgaa aaggtaatac gaaacaaaca gcgcaagcgc attgttcgtc
     9061 atcattccgt tggcaaaaag ataaccgagg taataaatca gaagatattt gttatgcgca
     9121 agatatgtca gcattctctt gattgagaaa gcttcatcct caagctcgcc gtgatagttt
     9181 ctctccttgc cggtaaagca aatcgggatc ataaaaagaa gccctattac cgagagaaga
     9241 ataaccgtcg ggctgaagct cataccgacc ttttcgctga caagcagtgt gcaaagcaat
     9301 cccgaaatgc ccatgcccgc actgctgaac agtctgccga tggacataag tgtattgcgc
     9361 tcgttgacat tatctgtcat cgtcgttacc atgctgtaaa tcggaacgtc acaaaccgta
     9421 tagctcgaat cccaaagcag ataagctatc ataaaccata tcagcttgcc cgtctgtgcc
     9481 atgccgctcg gaattatgaa caaaagaacc gtagtaatcg gaacaagtcc ggcggaaata
     9541 cgaatccaag gcatacattt ttgcttgttt ttgaatttaa ttttatcgaa aattatacca
     9601 aacatcgggt cgttcacggc gtcccaaatc ttgacaatca acgtaacaag agcgattttg
     9661 ccaaggtcaa ttccgttcat catcaaatag gttgccatga atgtcgttac aacattatag
     9721 aaaatattct gtccgataaa atacgtccag tagctcacac gctcgccgag cttagttgta
     9781 taatcggcag gcgcgataat ttctttaatc ttcatgctat acccccttta tataattata
     9841 cattttttac aaattgccgt caatgataat atataacaaa acggattgcc gcagtttatg
     9901 caacaatccg tatttttata ttcttatcag aagctcattg tatgggtgca aatccacttg
     9961 ccgtgtgccg acgctgtgac taccatgccc ttaacgcccg gaatattctt ggtaacaacg
    10021 cccgtgccgt ccttaacggt gaggggcata tcaactctca cgtagctaac acgtcctgcg
    10081 gcgtcgatat tcgccgtgag cattgtgcct ggataattaa actgagcctt tgtgatctct
    10141 gcaaatccgt tcaaagcacc tgcaacctca ctgatatcaa gcggccatgc acatgatgcg
    10201 ttatagggcg gcttgctgtt gtgtccgcag ctttcggcaa cgagggttat aacaaccttg
    10261 tagccgtttc cgtttcttga aatgcttgcg cttttaacgc ctgccgagga aaggttggta
    10321 gggagaataa aggtgcttgc cgctgtgccg tcagaagatc ttccgtttga gaagcttttg
    10381 ctgtcctcgg tctgcttctg attgcctctc gcaatgctgt ttgcgatgct tgttaccgtg
    10441 ccgttttgct ttccgttaat tgttaccgaa tcaacgccga tagttacctg ctcggtcttt
    10501 ttgtcagtca tcctcgcctg tgcataggag ctttggaggc aggtgttata ataggcaaca
    10561 atctgatttt tgttatatgc gagaacattt gcagccgcct gagccgaacc gcccgtgcct
    10621 gtgccgcctg tggctgtgcc cgttccgtta ttggtagcga cgcccgttcc gttggcggtt
    10681 gtaacggtct gtccgttcgc atttgtaaca gtacccgttc cgttctgaac tgtgcccgca
    10741 gagccgttat tgctaacgtt gccggaggaa ttatcagtat tattcgactg acttatgtca
    10801 ggctcaatac ttcccgtaat cttatcaagc gcaacattcc aagccgtgca gccgataata
    10861 acgcaaagca tgaccgaaac ggttataata aatcttttcc agcttctgtt catgggtttt
    10921 acactctccc gtaatttatt atatttattc tatcatattt caaataaaat ttccactgtt
    10981 ttttttgata atccgaacac gttttacagg ctgaaatttg tatattcaat caccgttttt
    11041 gccgagccgc tgaccgctcc tccgctgagc ttaacgagag taacgtttga aagattgaag
    11101 cgctccgtgt agcttaccgt catggactga aggcttgaat cctctacgct gattcttgcg
    11161 actacgctga ttgaatctgc cgagacgctg accgaggctg cggacgactt gaattcaccg
    11221 agagacttgc ttacggcttc ggcgcttata taatctccgc cgaggcgatg aagtattccg
    11281 ttctcgtctg tcggattgct ttcgccgttg aactgagccg tcagcaccac aacatcgcct
    11341 tcctgcttgg ctgtaattga agatatttcc tccgtctcaa acttgccgct tataaaatta
    11401 tctgcaaaaa gctttttatc ggcattgtaa tcataatcgg ctgttatatc cttttcaccg
    11461 aaaacagaag caaaggcttc tgcggcattt gaagccgagg acagtgagcc gaggctgtca
    11521 atgctcgcct cacacttgag cgcctttttg tatttatagc agtatttgtc tacatagttg
    11581 acggcagcat tgagcatatc cgtcaatcca ttgctatccg taggaatgtt cgcaattttg
    11641 accgttttct cggttacgga tttttcatat gtcggttcaa cattctgcgt cggtgtttct
    11701 gtcttgcctt tacatgaagc aagcgagatg acaacgcaaa gtgccgtcag caaagataat
    11761 attttcaaaa aacgattatt catcttatca ccatagataa gattaaccta tcgcaggtga
    11821 tttgtcaagt gttatcctta ctgatggatt ccctgatcag cagtcggctt attgcctttc
    11881 cgttgaaggg aataagagga tagagatatc ccctgcctgt taccgttttc gtaaaggcga
    11941 taacaagggc aacagtcaag agtcctgcaa tgaagccata cagattgaat attgctgtca
    12001 tgacaactat gaatatccgc gaaagcttga aggcgtagcc caattcaaag ttcggctgag
    12061 caaaattagc aattgcgaca aatgccatta tcagcacgac ttcggac
//
LOCUS       DAWBSW010000044        12018 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1155_length_12018_cov_2.922093, whole genome shotgun sequence.
ACCESSION   DAWBSW010000044 DAWBSW010000000
VERSION     DAWBSW010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1155_length_12018_cov_2.922093"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3485..3494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6887..6896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10555..10564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11690..11699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3128 a         2963 c         2412 g         3475 t
ORIGIN      
        1 gcaatatttt tgaaaatctg ctgagacaaa atttcagatt cggacgaatg taatttttct
       61 ttgagctaaa acattacaaa tattttttta tcaagaaagg aaaattacca tgtcagatta
      121 taaatttgaa acacttcagc ttcacgttgg tcaggagact cccgacccgg caacagattc
      181 aagagcagtt ccaatctatg caacaacttc atacgttttc aaagactccg cacaggcggc
      241 cggccgtttc ggtttgaccg aaggaggcaa tatatataca agactgatga acccgacctc
      301 tgacgttttc gagaagagaa ttgcggctct tgaaggcggc gcggcagcgc ttgcgacagc
      361 ctccggttca gcggcaatct catatgcaat tcaaaacatc gcaattgcag gtgaccatat
      421 cgtttcttcg accaatcttt acggcggcac acacaacctt ttcgcaaaca cacttaagga
      481 gcagggactc gatacaactt ttgtcgatcc ctccaatccc gaaaattttg aaaaggcaat
      541 tcagccgaac accaagcttc tatatgccga aacgctcggt aacccgaact ctgatgtaat
      601 cgaccttgaa gccattgcag ctatcgctca taagcacggt attccgttca tcgttgacag
      661 caccttcgca acgccttatc ttcttcgtcc gattgagcac ggcgcagatg ttgttgttca
      721 ttccgctaca aaatttatcg gcggtcacgg cacggtaatg ggcggcgttg ttgttgattc
      781 aggtaaattc gactgggcac agaacgacaa gtttcccgga ctttcaaaac caaacccgtc
      841 atatcacggc gtcgttttca ccgaggcttg cggcaatctt gcctatattc tcaagcttcg
      901 tacaactctt atgagagatc aaggcgcaac gatttcgccg tttaactctt tccttcttct
      961 ccaaggactt gagactcttt cgctcagagt agagcgtcac gttgagaatg cgctcaaggt
     1021 tgttgacttc ctcaaaaatc atccgcaggt agaaagagtt aaccacccct cacttgccac
     1081 aggcaagcag aaggagcttt acgacaaata cttcaagaag ggcgcagctt caatcttcac
     1141 ctttgaaata aagggcgatg cagaaacagc caagaagttc accgagagtg tcaagctttt
     1201 ctcgcttctt gcaaatgttg ccgacgttaa gtcgctcgtt atccatcctg catcaacaac
     1261 ccattcacag ctttccgaag aggagcttct ctcctgcgga atcaagccga acaccgttcg
     1321 tctttcaatc ggcacggaac acatcgacga tattatcgca gaccttaaac agggttttga
     1381 tgcggttaaa taaaacataa attaaattct tccttctctt tttctccccc tataaaacaa
     1441 agactccgat ttattcaacc ggagtctttg ctgtttttat aacctttaat ttgaatacgg
     1501 atggaaaaat ctgttgaata cattatatcc gagcgcaaca tatttaaaca gccagataag
     1561 cggattgaat cggaagaaaa gccaatccgt tacggttgca agtccacgca gaaatgttga
     1621 aacatcgtgt gtttcgggaa catcaacggg agtaaattcc tcgccctcaa cgctgagcac
     1681 gaacggctga gcgtaacaaa taccgttctc gcccgtaata taaaatctta tataaagata
     1741 aggatcgtcg agcaagtcat cgctatagag atccagcatt gcggttccgc ttgtgatatt
     1801 ttcctcacgc ttgataacgt ttccgtttga aacccatgtg atgcgatcaa agtttgtgcc
     1861 ctcaatcttg attgtaccgt tatcccggtc aacatcaatt cgggttacca tcggcgtgtt
     1921 atcctgagaa taaagttttt cgtcgtaaac cttatcttca tcgaagcccg gaatttcctc
     1981 catgccgtta agctcaacgc cgtttgaata atgcgatacg gcaaagctcc aaccgttttc
     2041 catgcttgca cggaaattgt tcatgtcgaa atccttcatc ataagcattg tataggcatc
     2101 attgagagat cttacattat gagaatccga gaaagcaaat ccccacggaa cgacgccgtt
     2161 cggaatagtc ttttgaagaa tctgatcata gagaattctg tcgcaacgag tatgcgcatc
     2221 ggttgcactg ttgatgccaa tgcctaagct tgagcccgca ttgtcaagaa aaagacgtgc
     2281 aaacttgttc gcataataat cgtcaacttt ctgaccgaca tgatctgcac tgtccttatc
     2341 gggataaaca tattcgccga catgagagag catcgaaatt ccgccggctt ctcttacgcc
     2401 cttgctcggg gtctcataat cgccgtaaac gccggcaagt ccctgaccgt aatccgaaaa
     2461 atatcccgta aggtgacagt ccgccttggg cgttgccatg ttaagctcta tgccctgcgg
     2521 aacatccaac attccgttct tatgcgttct ctcggcagaa ggcgcagttc cgctctgata
     2581 ggattcatat tcttcatccg tcaacggaat aatgggagcc atgtgcgttc tatcatactt
     2641 aacaagtctg ataagcggaa ccaggtcggg cttctgattc cagcccttgt ttatcgttcc
     2701 gtgatccgtc agcgcaacaa tatcatagtt gaggtcatag tgcatctgaa cagattcttt
     2761 aaccgtgaga tatccgtcgc tggcgtttgt atggcagtgc ggttgaaatt tgtagcttcc
     2821 ccactcatcc caatcaacct tctcataggg atttacaatg atgtactttt tcccttttgc
     2881 cgatacaccg attacaaaaa gctgtgcgga aagaacaatg ctcaaaaata ctgctaaaat
     2941 tttctttgcc attatgtttc cctcctcaaa aaggtctttt ttctattatg tctgactttg
     3001 caggatttgt caacaattga atattctttt tttaaataaa attgcaaacc tattaaaaaa
     3061 acaagccgaa tcttttatcg gattcggctg aattttatta ttttctttga ttttttctct
     3121 ttatttcatg aaggcgttcg agctcttcat tgcgttctct ttcaatcttt ttctttgcgt
     3181 tttcttcttt ttttgctttt atttttccgc ttatgtcaaa ttttatctga ctgagcacaa
     3241 tgcaaattat aacgacggca acgagtgcga ttatccatat cgccttttgt ttctcatcat
     3301 tgttgttctg caccggctca agctcgctga cgctgatagc caaagtgttc aaaaaataat
     3361 taaacaaaat cactcatctc cctgtatagt ttcgcaatcg gcaatcccat tacattaaaa
     3421 aaatcgccgt tgattttttt aacaagtacc gagccttttc cttgaattcc gtatgcgcct
     3481 gcctnnnnnn nnnncgccga gcgggtaaaa ttccacgtcg gtttcatcaa agaagctttt
     3541 ctccttgccg tcaccgacta ccgcgacgcc cgtgaaaacc ttatgagtcc tgcccgaaag
     3601 caaccgaagc atttcgcaag cctccgactc cgaatgaggc ttgccgagaa tttttccgtc
     3661 cagtgcaact accgtatccg agccgacaac aaccgcatct tcattctctg ccgcaaccgc
     3721 ctgcgccttt tgcagtgcga gcgacataac aacctcctgc ggctgaacat tttcgggaac
     3781 ggcctcaaca atatcagcgc agatcacctc aaagtcatat cctgcaagct ccatcagttc
     3841 tctgcgtctc ggcgaagcgg aagcaagaat tatccttgac atcaccacag ccccttatct
     3901 ctgagctgtt tgaccttaag aaccgccgcc tgactttttc cgtccttgat ctcgttgctc
     3961 atgaccattt caaccgccgt ttcaagcgga attttttcaa cgttgaggaa ctcgtcaacg
     4021 tcaagatgct ggtgcgtata ggtcaagccc tttgcggcat agagatgaat gatttcgccg
     4081 caatatccgg gtgacgggta aaacttgcca agatcataat actcgtcggc aacacaaccg
     4141 gcttcctcct caagctcacg cttgcccgtt tcaaacggat cctctccctt ttcaagcttg
     4201 cctgccggaa gttcgggaat aacttccata tacggatagc ggaactggcg gacaaaaagc
     4261 agctcgttat cctctgtcag tgcggcaatg caaactccgc ccggatgctc aacaacctca
     4321 cgccggcatg gctttccgtt cggaagctct gcatcgtcat tacgaacatt gattattctg
     4381 ccatgataaa tataattctt tttaactgtt ttttcttcaa gcttcatgat tttctccaaa
     4441 agaaaagggg caaccgagga aacgaatttt cgcccaaggt cacccccgtt tttaaaaatg
     4501 attatttgtc gtcgccgtcg tcctcgtcgt caaaatcgaa ctcaagagtc tcgccgcaag
     4561 cggggcactg aattgattct tcatcaagaa cactctcatc aaggcagatt gtctcgccgc
     4621 aagcggggca ctcaacctca aaatagtcgc tgtcgtcatg atcgccgcag caacaatcat
     4681 cgtcacagca gcagtcgtca tcgtcgcaat aatcgtcgta gatgcactcc tcaacgtcgc
     4741 cgagatcctc atcgatctca tcaacaacct ctgtgagagc tgcgacctcg tcctcgagat
     4801 ctgcaattgt gagagccatc tcatcaatag cgtcaaccat tgccttgatg attttaactt
     4861 catctgtatc tgcgtcaaaa tgagccatct caacaagacc cttgagatat gctgcttttt
     4921 cggtaactgt catcattgtg aatcatcctt tcttttaggg taaataaatt gcttatgctc
     4981 tgccgagata ctcgcctgta cgggtgtcga tctgaacctt ctcaccctcg ttgataaaga
     5041 gcggaacctt gatctctgca ccggtctcaa gagttgccgg ctttgtagcg tttgtagccg
     5101 tgttgcctgc gaagcccgga tctgtctttg caatctcaag ctcaacgaat gtcggcggtt
     5161 caacgccgaa aaccttgccc ttgtaggaaa gaatcttgca aaccatgccg tccttaacaa
     5221 acttaaagtt atcggaaaga tcagaagcat tgataggaac aagctcatat gactccggat
     5281 ccatgaagta ataaagatct ccgtcgttgt atgaatattc catgtccttt ctctcgataa
     5341 aggctgtcgg gaatttagct gtcgggttgt agctcttttc aactacggaa ccggtgagaa
     5401 cgttctttgt ctttgttcta acgaaagctg cgcccttacc cggcttaaca tgctggaatt
     5461 caacaacctg aagtacgttg ccgtcctcct caaatgtcat accgtttctg aaatcacctg
     5521 ctgaaatcat gtgttattcc tccgatttat tttattcgtt atgaataggt caaacaccta
     5581 ctcgtatttt atctctatta ttttatacta ttatcgtgta tttttcaagt cttttcagct
     5641 aaaaagcatt atagcgcact tattttttta caaaattaca agctctttcg gtgcatttgt
     5701 aaggtttttg cagccgtttt cggtaatcag ggccatatcc tcgattctga cgccgaattt
     5761 cccggggata taaataccgg gctcaaccgt cacgatattg cccgaatgaa gcgttgctgc
     5821 cgacttcgga gaaaggttcg gcttttcatg gatttcaatg ccgacaccgt gccccgtcga
     5881 atggcggaaa aattctccgt atcctgcttc gattataaca tccctcgccg ctttatcacc
     5941 gtcgaagcat ttaagtccgc ttcttaaaac ttccagagag gcaagctgag ccttcaatac
     6001 ggtatcatag acttttttct gctcgtcacc gatatggccg accgcaaccg tccttgtcat
     6061 gtccgaatga tagccgttaa caacagcgcc gtaatccatt gtcacaaaat cgccgtcctc
     6121 aattctttta tcggtcggaa cgccgtgcgg ctttgacgta tttgctccgc tgactgctat
     6181 tgtctcaaat gaaagcgcct gtgcaccgtg gctgagcata taatgatcca gttccagtgc
     6241 aatttctttc tctgtcacac cggctttgat aaagccgaga atatgctcaa gtgccgcttc
     6301 tgcaatgcgt tgagccttgc aaatattttt gatttcttcg tcggttttta tcgctcggaa
     6361 cgagtcgatg gttttatctg ttccgaccgt cgtgatcctt attccgcgaa gagcttttct
     6421 cagattttta agctgagtaa cggtcagtct gtccgcctcg acagcaaggg ctttgatgcc
     6481 taatcgtttg gcatattcaa tcagacctgt tttttgttcc tcgacttcgc agcaatttat
     6541 tttgtccttt gccgcttcaa tatatcggct gtcggtcagg aaaattgaac cgttttttgt
     6601 tgcaaaaagg aatccgtccg atgcgttaaa gcccgtaaaa tactgtctgt tttcaggcga
     6661 aactatcaat gcaccgtcaa catttttcgg cagttttttt aaaaattcat caatcatttt
     6721 atctcactcc tatatcagct cggcaataat tgaatttgaa agaagaaatc ctttttcggt
     6781 cagagccatt gatttttcat cgcatttcat caattcgttt tttaaaaact tttccgtctt
     6841 ttttattata tcacgcggaa atgcttttcc gaatctcttt tcaaaannnn nnnnnngcat
     6901 aatatattct tcctcgtctc cgccgtttcc gtcgggaatc ggttcccctc cgtttttaaa
     6961 atattgaata tctctcggat aaaaaaatct ttttccgtca ataaaagaat gtgccgacgg
     7021 tccgagcccg aggtattcct cgcaattcca gtatttcaaa ttatgctttc cctcatatcc
     7081 ttgacgggca aaatttgaaa tctcgtaatg ctcaaatccg tttgacagaa gcctctcgct
     7141 cattgcaaga tacatgtcgg caacctcatc gtcatcgggc aaattaagcg aatccctttt
     7201 tttataataa gttgttccct cttcgatttt cagcatataa gcgctgatgt gttttgcgcc
     7261 tgacgcaatg caaaaatcga gcgtttcgct aagacttttc tgcgtctgat cctgcacgcc
     7321 gagcataaca tcaagtgaaa tgttttctat tcctgccgat ttgcattgaa tgattctttt
     7381 ttcgatgagt tccctgtcgg aaaacctgcc gagctttttt ctttcgcttt cgattaccga
     7441 ttgaacgcca agagaaattc ggttaactcc ggcatcggca actgtttcaa aaaaatcctc
     7501 ctcaaccgcc gaggggttac attcaaccgt aatttctccg tcaacgccga aaagattttt
     7561 tgcgcgccgt atgagcatag caatgtttgc cccgccgaga accgacgggg tgccgccgcc
     7621 gatataaatc gtatcggctt ttctttcaag cctgtccgac caagcttcca tgctttcgac
     7681 aagagaaatc atatacatat ctttatcgga ttcgttgcct cgcatagaat agaaatcaca
     7741 ataagggcat ttggttttgc aaaaagggat atgaagatat aatccaatat tattcatggc
     7801 ttctccgaaa aaaacggact gttctttcga gcagtccgtt gatttaataa attatttctg
     7861 ttcagcagac gcctccgcca tttcaagctc gtgagctttg ttaagctcct tagctgtttc
     7921 ggcgcgacgc tctgcaagct ctttgtacat tctcgctctt gtctcggagt cgatgttgta
     7981 gaagagcttc ggaatgatac cgataagacc cgttgccgcg ggaagaagag tagtcattgt
     8041 gaacaggagt ctctctgttc tgtcactctg ctgctttggc ttaaggccct gtttgtatcc
     8101 gatgataccg agaagagctg aactgaagcc gttggtaaag gttgcgacca gctttctggc
     8161 aaggccttta acaacacccg ccatagcctc ggtacggtag ccgttctgcc attcgccgta
     8221 gtcaatggct tcgtttctga tttcctcggg aacaacctta cgcatagcgt aaacaaagtt
     8281 ccaaagagtc tctctaatca taaacgcggc aatcatggga atggtcttct tatagttctt
     8341 gtttatgctg ccgaataggt aaactccgag atagaggaag tcaccccagt gagaaccgat
     8401 tgcccacaag gttctcgttg agaacttctc acgaagctta tcaacataag catatgaagc
     8461 gtatgtaaca aacattccgg gaataccaac gatcagcttt gccgatccgg cattcaaaac
     8521 gctgatataa tacagcgaaa tattggttcc gacagcaaac tgtccaagga actccgaaat
     8581 agtcaaaagc ataagcggac gagacttaaa cagcgaaccg accgcaccct tgaaggttgg
     8641 tttttcgacc gattgaagaa ctctttcttt tactttaaag gagaagaaaa gagccaaaat
     8701 acctgcgcac agcgctgtgc cgacacccat ataaacaaac aatgatgtca tcttgatctt
     8761 gagcttgttg tggttaacaa ggtcaatgag aaccgtacat acctggttcg gaatattttc
     8821 aacaagactt gagaacaggt tcgcctggtt gataagacgg gttctgtcga aaacatccgg
     8881 tgtaatcgtt gcaataatac ctgttttcga gatctcataa agcgaggtcg ccaggttttg
     8941 cagcatttgc agcacaaaat acgttacgat cttcggcaca tatgtacccg aggtttcggg
     9001 gaacataaca ggcatacccc agaaaatcat tgccatgatt gttcccggga gagcataaat
     9061 aataagatac ggtttgaatt taccgaatct cgtgtttgta cggtcaacaa tagacgcaag
     9121 aaacaaatca ttgatgatat cccagatact gacaacaaca ctgatgattg cctgccacca
     9181 ataggagatg acaagaacgt cagtcaggta atatgtcttg ttctgatcca gattgaagct
     9241 ctgagcaata tcgaaaagaa cataggtgaa agtttctcta cttccgacgt aacgtctgcc
     9301 ctttggcagc ttacgtttgg cagccttctg ctcctccggc tctgcctgag aggtctgaac
     9361 ttctttagtt tcagcagcca tgctttacct ccttactgat ttatacaaat ttgcttttac
     9421 attatagcac aaattatatt caatatgcaa cagttatttg aaaattttta gtcaaattaa
     9481 attattcgtc aagtttcaga attgacataa acgcttcctg aggcacctca accgtaccga
     9541 actgtctcat gcgctttttt ccttcttttt gcttttcaag cagctttttc tttctcgtta
     9601 tatctccgcc gtagcacttt gcaagaacat ccttgcgcat tgccttaatc gtttcgcggg
     9661 caataacctt gcccccgacg gcaatctgaa caggaatttc aaacatctgt ctcgggatat
     9721 tgtccttgag cttttctgca atttttcttg ctctgccgta tgccttttcg ctgtgaatga
     9781 taaacgaaag cgcatcaatc atgtcgccgt tgagaagcac atcaagctta accagatttg
     9841 atcgggcata accctcgatt tcataatcaa acgacgcata gccttttgtt cttgacttca
     9901 gcacgtcaaa aaaatcgtat ataatttcgt tcagcggaag gtcatatgca atgtcaacac
     9961 gttccggagt aacataggtc atgttcttga aaactccgcg acgttcctgg caaagatcca
    10021 taattgcgcc gacgaactca ttcggagcat atatatttgc gttggttatc ggctcttcgc
    10081 aatagtcaat aaccgcagga tcgggatatt ttgttgggtt gtcaatttcg ataaccgatc
    10141 cgtctgtcag gtgaattttg taaacaacac tcggaaccgt ggttacgaga tcaaggtcat
    10201 attcacgctc cagacgttct tgaataattt caaggtgaag cagtccgaga aatccgcagc
    10261 ggaaaccgaa tcccaatgcg ccggaggtct cgggttcaaa ggaaagcgaa gcatcattaa
    10321 gctgaagctt gctcaaagca tcgcgcaagg cttcatagtc tgctccgtcg gcaggataaa
    10381 caccgcagaa aaccatcggg ttgacctttc tgtaaccggg aagcgcctcc tctgcaggat
    10441 attccgcagt cgtaacggta tcgccgactc ttgcatcgcc tactgttttg attgaagcgg
    10501 ttatatatcc gacctcgccg gaggtaagct ccggtgccgg ctccattgat gttgnnnnnn
    10561 nnnntcatgt agccgacttc aacgacggta aattccgccc cggtcgccat cattcgcatt
    10621 gtatcgccgg ctttgatttt accgtcaaca acacgcacat aaacaataac gcccttgtaa
    10681 ttatcataaa ccgaatcgaa aacaagcgct ctgagcggct tgctgtcgtc ggaatccaac
    10741 ggagccggaa tatcggaaac tatcttttca agtacagctt cgacgttttc gcctgtcttt
    10801 gccgaaaccc tcggagcgtc ggagcaatca aggcctataa cttcctcaac ctcctccgca
    10861 acacggtcgg gatctgcggc cggcagatca attttgttaa ttatcggaac tatctcaagg
    10921 tcatggtcaa gcgcaagata tgtgttggca agtgtctgcg cctcaactcc ctgcgatgca
    10981 tcaacaataa gcaatgctcc ttcacatgcg gcaagcgaac gtgaaacctc gtagttaaag
    11041 tcaacatgac ccggggtgtc aatcagatta agctcatatt cctcgccgtc cttcgccgta
    11101 tagttgagtt tgacggcgtg cgccttgatt gtaattcctc tttctctttc gagatccatg
    11161 tcatcaagga gttgctcggt catgtcacga accgcaacgg actgcgtcaa ttcaagaaga
    11221 cggtcggcaa gggtggactt gccgtggtca atatgcgcta ttatcgaaaa atttcttata
    11281 tgatcttttt ttactgccaa aatatcacct ctgtgacaaa cttatataaa actcaaaaca
    11341 ggacaggcta tttgaaccga tagcctgtcc gggtttttat tatttatttt caaaagctcc
    11401 tctgaaaacc gaagctactc tttcaagatc ctcctcggtc attgccgttc ccgagggcaa
    11461 acaaactccg cggcggaaaa tatcatttcc gacgctttca ccctgcttgc agcttatgaa
    11521 atccatttcg gaaaagatag gctggttgtg catcggattc catgcgcgtc ttgattcaat
    11581 attgttatcc tccagctcct tgattatctc gtccggggta acatcacagt cctcgtttat
    11641 tgtcacaacg ctaagccaaa aattcggctt gcagccgtca agaacgggan nnnnnnnnnt
    11701 caccggaaga tcggcaaatg tctttttata aaactcataa tttcgaactc ttgcggcaat
    11761 tctttcgtcc atatttttaa gctgtcccct gccgatacct gcacaaatat tggacattct
    11821 gtagttataa ccgatttcat tatgaagata gtaattaacg ttttccttgg actgatttga
    11881 ccaaaaatgt atctttttaa tagcttccgg gtcatcggaa agagccattc cgccgccgga
    11941 ggtagttaca attttatttc cgttaaagga cagcgcgctt accttgccga atgttccgca
    12001 tttttttcct ttataggt
//
LOCUS       DAWBSW010000045        11586 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1207_length_11586_cov_3.026884, whole genome shotgun sequence.
ACCESSION   DAWBSW010000045 DAWBSW010000000
VERSION     DAWBSW010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1207_length_11586_cov_3.026884"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1357..1366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3038 a         2771 c         2600 g         3167 t
ORIGIN      
        1 gttattacct cactttatta tttgctgagt ttttttaccg tttccatcac atagtcgccg
       61 aattcggagc atgtgcagcc cgtgtcgcga cccgtaattg tgagcttttt ctctgtaaac
      121 atacagatgt cgagagcctt ttcgagcata tctgcctttt cctgctctcc gatatgggag
      181 aggagcataa ccgaagctct gagcattgag cacgggtcgg catactgacc tctgccttct
      241 tcaatcattc tcggagccga accgtgaatg gcttcaaaca tggcatactt cttgccgatg
      301 tttgcactgc cggcagtacc tacgccgcct tgaaattctg cagcttcgtc ggtgataatg
      361 tcggcgtaaa ggttggggag aataaatacc ttgaaatcct tgcggcgctt cgggtcgatg
      421 agcttagctg tcgtgatgtc gatataccac tcatcggtcg taatctcggg ataatccttg
      481 cctatgttct tgcaaagatt gagaaattta ccgtcggttg tcttgataat attggccttg
      541 gtgataattg aaactctgtc cttgtgattt cttcttgcgt agtcataggc gagctttgca
      601 attctctcac agccctccgt tgtggttaca acaaaatcaa cagcgagatc gtccgttacg
      661 ttaactccct ttgaaccgac agcataagca ccttctgtgt tctcacggaa gaatgtccag
      721 tcgataccct cctcaggaac cttaacggga cgaagatttg cgaaaaggtc gagagccttt
      781 ctcattgcaa cgtttgcgct ctcaatgttc ggccacgggc cgccctgctg aggagttgtt
      841 gtcggtcctt tgagaataac gtggcatgat ttgagttctt caagaacgtc gtcgggaatt
      901 gccttgttgg cggcaacacg attctcaatt gtaaggccgt cgataacctt gaactcgacc
      961 ttgcccgtct taaccttatc ggcaagcata tattcaagaa ctcttgcgct ctccttggtg
     1021 ataatcgggc cgattccgtc accgccgcaa acgccgatta caattttatc aagcttttca
     1081 tagtcaacga aatcgccctg agccttgatt ttatcggaac ggtcgctctg ctcacggata
     1141 agagcctcaa atttttcaac tgcgttttta atctgtatat ctgtcattgt tttgctcctt
     1201 tacaatgtat tttgctttaa taccgacctc gggtcaatgc ggacaccgac ccctgcggac
     1261 tatgattttg ccccgcttcg gtgtgtcaaa gaagtttgat ggaaatatta ttcaggcatt
     1321 tatcaaagct tccaaattaa ggcgaagtct tgacaannnn nnnnnncgac tctcttgtat
     1381 aatcaagcaa tccaccggca aggagaatat ctctctgacg ctcggaaagc tgatagtcaa
     1441 gcttatagct ctcgcctgtt gtgaggttct tgagcgtaac atcagtaccg tttgcgatgc
     1501 tctcacgaat tccctcaagg caaagctcgt catttacgct gattttgtcg taatcattct
     1561 cgttcgcaaa ggtgaacgga ataatacctg cattaacaag gtttgcacaa tggatacggg
     1621 caaacgactt ggtgatgacc gccttaattc caagatagag cggaacaaga gcggcatgct
     1681 cgcgtgatga gccctgtccg tagtttgcac cgccgatgat gatgcccttg cctgccgcct
     1741 tgcaatgctc tgcaaacttc tcgtcacact gcttgaagca gaagtttgaa aggtacggaa
     1801 tatttgaacg gtatggcaaa atctttgcac ctgccggcat gatgtggtct gttgtgatgt
     1861 tgtcgccgac cttgagaaca gccttggctg tgatgtcctc gggcattgcc tcggtcttgg
     1921 ggaaaggctt gatgttcggg ccgtacataa cctcaacgga atctgcgtcc tcaacgcttg
     1981 cgggcattgc gatcatgttg tcgtttataa taaactcgtc cggcatctcg atctcgggca
     2041 tttcaccgag ctcacgggga tcagtaagaa cacctgtaag agcagaagcg gctgcgacct
     2101 cggggctgac aagataaatg cctgcgtcgg ctgtgcccga acgtccctca aagttacggt
     2161 taaatgtacg aagcgatacg ccctttgaat tcggcgactg acccatgcct atgcacggac
     2221 cgcaagccga ttcaagaatt cttgcgcctg catcaatcat atccgaaagt gcaccgtcaa
     2281 gagcaagcat attaagcacc tgctttgaac cgggagcgat tgaaaggctg accgacggac
     2341 aaacagtctt gcccttgaga atagcggcaa ccttgagcat atccatgaga gatgagtttg
     2401 tgcacgagcc gatacaaacc tgatcgatgt gaattttgcc tatctcccgg cagctcttta
     2461 cattatcggg catatgaggc atagctgcga gcggaacaag ctcgttgagg tcgatatcaa
     2521 taatctcatc gtaaaccgca tcatcgtctg cgctaagctc aatccaatcc ttttcacgac
     2581 cctgagcctt gagaaaagcc ctggtgatat catcggacgg aaaaatagag gttgttgcgc
     2641 caagctccgc tcccatgttt gtgattgttg ctctctcggg aaccgaaagc gtcttgacac
     2701 cctctccgcc gtattcaact atctttccta cgccgccctt gacggacatg atctgaagaa
     2761 ccttgaggat aatatccttt gcggcaaccc acggctgaag cttgccggtg agattgacac
     2821 gaaccatttt cggcattgtt atgtaatatg cgccgccgcc catagcaaca gcaacgtcaa
     2881 gtccgcctgc gcccatggca agcataccta ttccgccgcc tgtcggtgtg tggctgtcgg
     2941 agccgataag agtttttccg gggataccga agcgctcaag atgaacctgg tgacaaatac
     3001 cgttgccggg acgtgaaaaa cgaagacctc tcttctttgc gacagactga ataaagcgat
     3061 gatcatcggc attttcgaag cctgtctgca atgtgttgtg gtcgatatat gcaacggaaa
     3121 gctccgtctt aacacggtca attcccattg cctcaaactc aaggtatgcc attgtacccg
     3181 tggcgtcctg agtcagagtc tggtcgattt taagaccgat ttcactgcca accgtcatat
     3241 tgccggaaag cagatgattt ttgataattt tctgagctat tgtgtatccc atagcgttga
     3301 aacctccgac ttgttcaaat ttttaacaat ctttatttta ttatataatg ccttatttgt
     3361 caatgttttg acagtatttt ccgacaaaaa tttaaggcga tatcacataa aatatacagc
     3421 gcaccttgct caaatccgct tccttcacgt tatattaaaa ctccttgcat atacaagaat
     3481 aaaatctctt taagagacag aagaaaacta aaaagcaaag ccataatcca cactaagcgc
     3541 acatcattgc cgccgcctgt gaattatagc ttttttcttt ggtctatgca aaatgcacca
     3601 accccgtgta ttcttatccg ccgaggtatg ccttcttaac attctcgctc ttcataagct
     3661 cctcgcctgt gcccgaaaga actatctttc cggtttcaag aacataagct ctgtcggaga
     3721 tcgcaaggct cttacctgcg ttctgctcaa caagaagaat tgttgttccg tcattattta
     3781 tatccttaat gatttcaaaa acctgatcga caagcagcgg agaaagaccc attgaaggct
     3841 catccatcat aagaagcttc gggtggctca taagagcacg tcccattgca agcatctgct
     3901 gctcaccgcc cgagagtgtt ccggcaatct gattttttcg ttcttcaaga cggggaaagc
     3961 gagtgtatat cttctcaata tcatccttga tatttttctt atccttgagt gcgaacgcgc
     4021 ccatgagcag attatcgtaa accgagagct cctggaaaat tctgcggcct tcgggacact
     4081 gagtcatgcc catttcaacg attttgtgac agggagtttt tgtaatgtcc ttgccctcat
     4141 atgtcacgtg accctcacga atcgggataa gtcccatgat cgactgcatc gttgttgtct
     4201 tacctgcgcc gttggctccg ataagcgtaa ctatttcgcc ctgattaacc tcaaagctga
     4261 tacctctgat agcctgtata acaccgtaat agaccgaaag gtctttaact tccaacattg
     4321 ccataacatc aacctccgat ataagccttg acaacctcgg ggtcattaag agcgtcgtct
     4381 accttgccct ggcagagaac cgtgccgaag ttgagaacgg taatctcatc gcaaagaccc
     4441 gaaacaaatt taaggtcatg ctcgatgaga agaattgtaa tgtcaaaatt gtcacggaga
     4501 tatcttaccg tgtccataag ctcctgagtt tcatgaggat tcatacctgc cgccggctca
     4561 tcaagaagca aaagcttcgg attagtagca agcgcacggg caatttcaag ctttctctgt
     4621 ttaccgtaag gaaggttgca ggcaagattg tttctttcct cataaaggtc aaatatcttg
     4681 agaatttctt ttgccttgtc tctcattctg acctctgtat catagtgctt cggcagcttt
     4741 atcatgctga taaaggggtt gcacttaaac tcgggctgat tgtggagtcc tacaagaacg
     4801 tttctgataa cgctcatatt attgaaaaga cggatattct ggaaggttct tgcaatgccc
     4861 ttgtgattga tcttttcctg agttttgccc gtcaaatcct caccgtcaag gtaaaatgta
     4921 cctgttgtcg gcttgtaaac acctgtgagg aggttgaaaa ccgtcgtttt accggcaccg
     4981 ttagggccga taagaccata gatctgacct tttttaatct tcagattaac atcctgaacc
     5041 gctttaagtc cgccgaatga aattccaaga tttttcgttt caagtacata gtcagccatg
     5101 cttatttgcc ccctttctga ggtttatcgg gtttgaacgg gtcgttctgc tttacatctg
     5161 tcgtaaggat ctcatccata cgaatctttg ttgtaacaac atcccatctg tttgtatccg
     5221 aatactgctt ttccggattt ttgaggcgct tctcacgaag cttgttagcg attgagaatc
     5281 tctctctgac gctcttgaga gcgggagcat tgttgaagat aacaacaaca ataaggatga
     5341 tggcgtaaac aagatacttg agtgccgcca ggtcgcccga aagctgagtc tggagctgga
     5401 aattgaggta tgtgataaat acagccgaaa ttattgagcc ggtgatatta cccataccgc
     5461 cgagaacaac cataacaaga atctcgattg aatagttgaa tgagaagaac gagtaaacaa
     5521 ccggagtaac atatgagccg taaataacgc ctgccatgcc tgcaaaaaat gcggcaaaga
     5581 cgaaaacaaa cagcttatag tatgtgatgt tgatacccat tgcctttgcg gcgatctcgt
     5641 tgtcacggat agccgtgatt gcacgaccgt gcttacttct gataatattt tgaataagga
     5701 aaagcgaaat cagaaccgta acaagagcca caatataaag gtccttgccg tacttcttgg
     5761 ttgaaagacc gagagcggca ccgaaagtcg gaagattctt aaatatggtt cttacgattt
     5821 caccgaatgc aagggtaaca attgcaagat agtcgccctt gagtcgaaga gcgggaagtc
     5881 cgataataaa tccgaatact gcggcaacaa gtccgccgat aagcattgaa atgataagca
     5941 caagaagctt tgaatccatt acattgtaaa gctcttttgc aatatagcat ccgatgtatg
     6001 caccgacgca catgaagccg gcatgaccga gtgacaattc accgaggaaa ccgactacaa
     6061 ggttaagtga aacagcaagc aaaatactgt atgccatctg agcaagcaag cctgcgcttg
     6121 agcggggaag atttccaaaa gtgctcataa gaagaggaac tgcaagcatt acaattataa
     6181 taaaataatt gaatgtatcc ttaattctta ttcctttttt aatattcttg agataattca
     6241 tagcttatac cttctccctt ctcttcttgc cgaggattcc gataggtctt acaaggagaa
     6301 taactatcag caaagcgaat tcaatagctg ttgcatacgg agcaagtacc ggaacgctca
     6361 aagcaagctt ctcaacaacg ccgagaataa cgcctccgag cattgcaccc gggattgaac
     6421 cgataccgcc gataaccgct gcagcaaagg ccttaatacc aggcattgag ccgagtgtcg
     6481 ggctgacaga aggaatctgc atgagataga aaacgcctgc acaagccgca agagccgaac
     6541 ctatagcgaa tgttatcata ataatcttat ttacattaac acccatgagc tgagctgctc
     6601 ccctatcctc ggaaacagcc tgcatggcac gtcctgtttt tgtatatttt acgaaaagtg
     6661 taagagcaac catgataacc gcaccgacaa cgagtgtaag aatcgtgtta agattaagct
     6721 ctgccgaacc gacattcacc atatgagttt cgctgattgt aacagcctgg ctctttgact
     6781 tgaaaatgag ctgagcaata ctctgaagaa gatagctgac accgatagcc gtgataagaa
     6841 cagcaagggg agaagcacct ctcagaggct tataagcaac cttttcaatg acaataccca
     6901 caaccgtaca aacaacaact gcggcaacaa cgccgagaag cggatgaccg tttgcattga
     6961 tggttacaag gatagcataa gcgccgacca tgatgatatc accatgggca aagttcagca
     7021 tcttagcgat accgtaaacc attgtgtagc ccaaagcaat aagagcatag atagctccta
     7081 agctgaatcc gtccaatatt gtagacaaca tttgatctac tcctttccgt tagattctaa
     7141 aaattaaacc gctgataaac cgatacctgc aaaccgtatt aaattggttt tcagatacag
     7201 attgatcggc ggtttaaaat acacctttac gggtgccgaa tgacacccgt aaagattgag
     7261 ttataaaatt ccgtatcttg gattaaagaa catccttgaa ctgaagttcc ttcttgcaag
     7321 cgccggaagc gtcccaggac attgtgcctg ttgcgcccgt gagtgtgaat ccgtcagctg
     7381 tgatagcagc cttaaccttc tcgcaaagat cgctcgggct gattgttaca tcattaacgc
     7441 ctgctgcctt cattgcattg aagatagcgt aaactgcatc gtaagcgtct gctgcaaact
     7501 ggtcgggagt ctcgttgtac tttgccttgt aggctgtaac gaacttgctt accgttgcgt
     7561 ccttgctgtc tgcatcaaaa ggagtgatgt acttaacctt atttgtaaca gtctttgaat
     7621 caatgaggtc tgcaataccg tcaaatccgt ctgtaccgac aaggtcggct ttgcagccct
     7681 tagctgttgc agccttgcag ataagtgaac cttcctgata gtacatcggg aggaagataa
     7741 cgtcgcaatc cttgagctgt tcgatctgag ttgagaagtc cttattgttc tcagcgtcga
     7801 atgtagctgc cttgaatgtt acgcccttag cgtccatctc tgccttgaaa gcatcgaaga
     7861 cacctgttga atatgtgtcg ctggtgttgt agataacacc gatattcttg tagttcttag
     7921 caagatcgtc tgcagcaaga gtaccctgat ccggatcacc gaagcaaaca cggaagccgt
     7981 tatctctgcc ctcaataacc ttagctgcgg aagctgacgg agtgatgtag aagaggttgt
     8041 cctcaactgc acttgcaccg aaagcatcgc aagaatcagt cgtaacggaa ccgagcgaga
     8101 tctgcatacc tgcttcataa agtgagttgt aaccggtggt tgcatcctga gcagttgcct
     8161 tgtcatcctt catatcgaac ttaaatttaa caccgttaag accgcctgcc gcattgatct
     8221 cttctatagc gatcgttgca ccgttcttta cggagttacc gtaggaagca ttatcgcctg
     8281 taagagggcc tgttgtacca atgaaatact ctgtgttgtt tgctgcgtag ttgtcacccg
     8341 atgctttttt gctgtcgctg ccttctttac cgccgcaagc ggagaagccg agagcaatca
     8401 aggcaagagc aagaactgcc gcaagaacct tttttactga ctttttcata aaatgacctc
     8461 cttttaattt ccgagctgct tttgtaactc ggtaaacatc atctatgatg tgaataatga
     8521 tacactctta atattgagtt gtcaacagat tatttttaaa aattacgtcg attttttaat
     8581 gaaattggct aaattcagag tctcgttttt gtgcactttt cacattacga aatgtaatgt
     8641 cattttttac aacatgaata tattcaataa ggggtataaa tatacaaatt gactaaaaaa
     8701 tatcatgatt tgagcgcttt aaagcgctgt tttattaaat cattcagaac tgagccttca
     8761 aagcatcaat cttatccgtg tgctcccacg ggagatcgac gtctgttctt cccatatgac
     8821 cgtaagccga agttgctgta tagatcggtc tgcgaaggtc aagctcgtcg ataatcgccg
     8881 ccggacgaag gtcaaaaaca ttctgaaccg cctttgcaag ctccgcgtcg gaatgaacac
     8941 ccgtaccgaa ggtatcgacc atgaccgaaa caggctttgc aacgccgatt gcataagcga
     9001 gctgaagctc acactttttg gcaaggcctg ccgcaacaat gtttttcgca atgtagcgac
     9061 acatataagc tgccgaacgg tcgacctttg tcggatcctt tccgctgaaa gctccgccgc
     9121 cgtgacgtga atatccaccg taggtgtcaa caattatctt acgtcccgta aggcccgaat
     9181 cgccctgcgg tccacctgtt acgaaacgac ctgtcgggtt tacataaatc tttgtgtttt
     9241 catcaataag tgacgggtca atcgtcggct tgattacctt ttcaataata tccttgcgga
     9301 ttgtttcaag ctcaacatcg gcgctgtgct gggaggaaac aactattgcg tcgatcctaa
     9361 caacccgatc gtcgtcatat tcaaccgtga cctgagtttt accgtcggct ctgagatatg
     9421 caagttctcc gttctctctc tcctctgtca gcttaagagc aagcttgtgg gcgagagaaa
     9481 tcggcatcgg cataagctcg ggcgtttcgt cacacgcata gccgaacatc attccctgat
     9541 cgcctgcgcc gttgagattg tacttatcct cctcgccgcc tttaagttca aaggaagcgt
     9601 caacgcccat tgcgatgtcc ggtgactgct tatcaagaga agtgatgatt gcacaggtgt
     9661 tgccgtcaaa gcccttgtcc gcactgtcat aaccgatatc gcagatagcc tttcttgcga
     9721 ttgacgggat atcaatattt gccgttgttg aaatctcgcc cataatgaga accataccgg
     9781 ttgtgcagca ggtctcgcag gcgacacgtc cgttcgggtc ctgagccatg atagcgtcaa
     9841 ggattgagtc ggaaattctg tcacatattt tatcgggatg accctttgtt acagattctg
     9901 atgaaaaaag atgctttgcc attttaaaat acctccattt tccgagccgc cgattaagca
     9961 aaacaagatg tcggcagccg ctccgttcgt aaaaaacgcc cccggcaaaa accgagagcg
    10021 tatatagcct tatctttcgg ttacaccgca ggatttagca ccttgccaat ggcaggttgc
    10081 cgagcttcac agggcctgtt ccctcagcta ctctgaataa ggggtgtttc gtttcacgaa
    10141 atctatcaaa ttgtatatgt gattatatac tatattactg taatagtcaa gtggtaaatt
    10201 atagttttta gaggtactgt cacttgcaag gggtgctgag cggttagtga acagtggtta
    10261 aagaaggtct gataaaattg ttttctgctg actaacttag aaatatgcag taacaaacaa
    10321 gggtgctgcc ccgtgacaaa taaaggtact gcccgagcga aaatgctatg ggcggtcgat
    10381 acctttaaac gggtttgaaa gaaaatcgtc cacagtgctt tttgcagtga gcggtgctgt
    10441 acaaaggtac tgccccgagt gaaaatgcta tgggcggtcg atacctttaa acgagtttga
    10501 aagaaaatcg tccacagtgc tttttgcagt gagcggtgct gtacaaaggt actgccccaa
    10561 acgaaaaaag tgctgtgggc gattttattc aaactattag atctttgtgt ttatcgagga
    10621 actgtatatc tacaaccgcc ttgacgtaaa ttccgtccgc acggtagtct tcctttatca
    10681 atacgccctc ttcacggata tagctgacaa ggccgccttc attataggga ataagaagtt
    10741 ccgcttttct gcgtgtgggc ggaagcgaaa gctgaatctt ggcaaggaga ttgccgagtc
    10801 cctcacccgt ctttgccgag atcatgacga actcgccctg tgctggaaga acgaatgatc
    10861 cgccgcagag gtcacattta ttcagcaccg aaataatagg cttatcggca catccgagcg
    10921 ccgcaagcag gttcattgta acattaagat gctcggtaca acattcgtcg gaggcgtcgc
    10981 agaggttgag aataaccgtt gcattcgccg cttcttcaag agttgagcga aaggcatcaa
    11041 cgagctgatg tggcaggcgt ctgacaagtc cgacggtgtc aatgagcatg accgttcttc
    11101 catcagggag agtcagcgca cgggaggtag gatcaagcgt tgcaaaaagc ttgttttcgg
    11161 cgagcacacc cgcattggta agcgtattca taagagttga tttgccggca tttgtgtagc
    11221 cgacaatagc aaccgtttca atgccgtcct tttcacgtct tgcacggaga ttttctcgcc
    11281 ttttcgcaat ttgaacaagc tcctcctgca ggctttgaat tcttcggtgg atatgccgtc
    11341 tgtcgctttc gagcttgctt tcgccgggtc ctcttgtgcc tattccgccg ccaagccttg
    11401 acatttccgc gcccttgccg ccgagtcggg gcaatgaata tttcaactga gccagctcga
    11461 cctggatttt gccctcgttg ctcctcgctc ttgcggcgaa gatatcgaga ataagcgttg
    11521 ttcggtcgat caccctgata tcggtctcgt cctcgatatt tctctgttga gacggggtaa
    11581 gctcac
//
LOCUS       DAWBSW010000046        11586 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1208_length_11586_cov_2.570636, whole genome shotgun sequence.
ACCESSION   DAWBSW010000046 DAWBSW010000000
VERSION     DAWBSW010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_11586_cov_2.570636"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10342..10351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3251 a         2675 c         2651 g         2999 t
ORIGIN      
        1 tcttgccctt cttgtagctt ctgagcatca tgttatcaac aatctccatg ttgcctacaa
       61 gtcccatacc gagtctgtcc tcgggaacaa aggaaagtct gatgccaagc tctcttatct
      121 gaacgggagt cttgtcacgg aggttatcct gcttgccggt ctttgcgtta ttataaatga
      181 ttgaaccgtg ggtaagatga cgaaggcctg cgattgattc aagcagctca cgctggccgc
      241 tgcccgcaat acctgctatg ccgagaattt cgcccgactt tgccgtaaac gaaacgtgat
      301 taagcacctt gacgccctca cggttgacat agcacatatt gttgataagg agtctgtcct
      361 ccttgttttc aacctctgtt ctctcgatgt tgaggtcgat cttcttgccg accatcatct
      421 ccgtaagagc ggattcgttc gtttcggcag tcatgaccgt accgatgtat tcaccttttc
      481 tgagaacggc aaccttgtcg gaaatctcca taacctcgtt gagcttatga gtgattatta
      541 taattgactt gttgtccttt ttcatgtttc tgataacgtc aaagagcttc tttgtctcct
      601 gcggagtaag aacggctgtc ggttcatcaa gaataagaat atctgcgcct ctgtaaagca
      661 ctttgacgat ttcaaccgtc tgcttctcgg aaacggacat ctgataaatc ttcttgttga
      721 ggtcaagttc aaagccgtat ttgtcaagaa ttgcctgaat ctttgctctt gattttttaa
      781 gattgtactt gccgtcatca aagccgagaa cgatgttctc cgttgcggaa aatacatcaa
      841 cgagcttaaa atgctgatga atcataccga tcttcaagtc aaaagcgtcc ttcggcgatc
      901 tgataaccac ttctttaccg tcaacaaaga tctgaccgtg atccgggaaa taaatgccgg
      961 aaatcatgtt cataagcgtt gttttaccgc ttccgttctc accgagaatc gagaggattt
     1021 cacccttgtt cacggtaaga ttaacgtcct tgttggcgac aacggagcca aaggtctttg
     1081 tgatgtttct gagttcgatt gcaacctgtg tactcacttt atgccctcca attttttatt
     1141 ctgaaaaatg tgataaataa tatattaaac tgattgatct tccccttgag gggtatactt
     1201 ggttagccca tatcttttta aaataaagaa accaaacaac ctgctttacc gcatttttca
     1261 aaataaaata cgaattataa aaatcaaagt aaggcggagc gataaggctc cgccttgaaa
     1321 ttgcgtaaaa tgattagaac ttagcgttga ggagattgat gccgtcgatc tgtacatcga
     1381 agtacggagc cgaacggaac tctgactcgt ggaagtagcc gtccttaaca gcctctgtgt
     1441 cgcctgtgaa gttagcgtct gtgtcaacgt ctgccatgta gcttgtgagc ttaccgttag
     1501 cgtcaacctt agcgttcttg ttcttgtcgg cagaaacagt tactgtgaat gtgcttgtat
     1561 cgaatacgtg aagtgtgccc ttatcaagct gagccttaac ctcgtcgatc ttagcctgtg
     1621 tatccttagc ggcagccttg ccgtttactt ctgtaagaac aacagagttt gttgcaagag
     1681 tacctgtcca gtctgtgtcg attgccttac cgtcctgagc gcacttgatc atgtactcga
     1741 agtacggctc ccagttgatt ctggaagaaa cgatgaatgt gttcgggcaa gcggagagtg
     1801 tgcttccgtt gtaagaaacg ttcggaacac ctgctgtctc acaagctgtc ggagcaccca
     1861 ttgaatcggc gtgctggctg atgagcttac aaccgttgtt gatgagcttt gttgcagctt
     1921 ccttctcaag agcctcatcg taccatgaac ctgtgaactg aacatccatt gttactgtcg
     1981 ggcaaactga ctttgcgccg agatagaatg aagtatagcc ggagataacc tcagcatatg
     2041 tgaaagcgcc gacataaccc atcttagcgt ccttagcatc aaacttgcct gctgcgatca
     2101 tttcgttgag cttgagacct gcagcaacac ctgcaaggta acgtccctca tagatcgaag
     2161 cgaaagcgtt gtggtagtta gcgatcttct cagtgtgagc cttagtacct gtagcgtggc
     2221 agaactgaac gttcgggaac tccttagctg cctttatcat gaaatcctca tgaccgaagc
     2281 tgtcagcgaa aataactgtg catcccttgt caacaagatc tgctgctgca tcgtagcaag
     2341 cgttctgctc gggaatgttt gtcttcttga gaatctctac gcccatcttc ttgcaggctt
     2401 cctcagcgcc tgtcataaag ttgttgtcgt aggttgagtt ctcgtcatgc aggaaaatga
     2461 atcctacctt gagcttcttt tcagtgtcgt tgccgccgtc cttcttggtg ccgcaagctg
     2521 cgaatgaaaa gaccattgcg attgccatga gaattgcgag aatctttttc atgtgttctt
     2581 cctccaaatt tttaatatat agcatttttt tgctaccgaa attatttgca gaacgttatc
     2641 ttgccgtcat cggtcatgga ctcgattctt gccagcgact caacacgaac gccctcactg
     2701 cgaatgagat cgccgccctg ctgaaacgct ttttcgataa ctattcctgc tccgacgatt
     2761 tgagcgcccg cagacttaat cagctcgata agtccgttga gagcacagcc ctgtgcgaga
     2821 aaatcgtcaa ttataagaat tctatcatcc ttattaagga attcctgcga gacgataacg
     2881 tcatacgtct tgccgtgggt aaaggacttg atcggcgtcg aataaacatc ggcagcgata
     2941 ttcttgccca gtgttttctt ggcaaagaca acaggaacat gaaaatactg cgcagtgatg
     3001 caggcaattc caatgccgga tgcctcaatc gtaaagatct tgttcactcc gcagtcctta
     3061 aaaagtctat aaaactcctt gccgatctca tttatcagct ctacgtctac ctggtggtta
     3121 agaaaactgt caactttcag aacattaccc ggaaacacct ttccgtcttt aagaattctt
     3181 tcttcaagaa gctgcatttc aaaccctccg aaataaaata agcagcaggg aacaagccct
     3241 accgccgaaa gaccgatcaa caacatattg tattcaaaac gctgtcaatc atctaccaat
     3301 agtgacagtt acggcactgt cgtagagact gccgggcctt ttatccggca atatatcggc
     3361 aatataatat tcttaagtat cgtcaatact ctggcgacag ttatatttta acacattatc
     3421 aacatttgtc aacactatac gacaacaaat catttttctc ttataagcat gctttttgtc
     3481 tattatcaac aatatttttc ttaaaatttt attcacatat tttttacatt ccgaatgtta
     3541 cgtatttatt gcattaaatc attcaatgtg ataaaatatc attgatttaa ggattattat
     3601 aattcgccga ttttccccct gtctactcgt ataatgagtt aaagggacgc ggctcaaaaa
     3661 agcaggaaca gataaaaagc tccgcatttt tcaggtaagc agcattgctc tgcggtataa
     3721 cataaataat gtatccgagg tataatcatg tcacatctta ttgaactaaa aaatgcatac
     3781 aaaatatata atccgggtga aaatgaggtt cgagcgcttg acggagtcga tctggaaatt
     3841 gaccaaggcg attttgttgc aattgtcggt cattccggtt cgggaaaatc aacgcttatg
     3901 aatatgctcg gtctgcttga cgttgtgaca agcggaacat acattctcaa cggcaaggac
     3961 gtttccgaca tggacgacga cgagctgagc agtataagaa atcttgaaat cggcttcatt
     4021 tttcagggtt tcaaccttat tccgagcctt acggctcagg gcaacgttga actgccgctg
     4081 atatacagag gtatgaaggc cgagcagcgc catgagctct ccgaagcggc gctcaaaaga
     4141 gtcggtcttg aaaagcgaat ggatcacctg ccaaatcaaa tgtcgggcgg acagcagcag
     4201 agagttgcca ttgcacgtgc tcttgccgca aagccgccga ttatactcgc cgatgagcct
     4261 acgggtaatc ttgattcaaa ttccggcaaa gatgtaatgc gtataatcaa tgagctttgg
     4321 gcggagggaa aaacgataat ccttatcaca cacgatgatt cgattgccga aaccgcacat
     4381 cgcataatca ggataagcga cgggaaaata gttgaggatt atgtcaacga aaacatcaca
     4441 cccgaaagta atttaaaata acaaatgacc gtgttaacga atacttgtaa ccctctcaac
     4501 agcaagttag cgttaggcag tgatcaagat ataaaattcg ttaaatttaa tttaaaaaca
     4561 ttaaaccgaa aaaaggataa tatgtctccc tctgcataga atagaaatat atctatttct
     4621 gtgaagaggg ttttgttatg tgcggaatta tgggctacat cggcgatgaa aacgcaaccg
     4681 acatcattat ttcgggtctt gaagagcttg aatacagggg ctatgactcg gcaggtcttg
     4741 ccgtctgtga cgggaaagaa atcaaaaccg tcaaggttgt cggcagtccg tcgggacttc
     4801 gtgaagaagc ggacggtata aagggtaaaa tcggaatcgg tcacacacgc tgggcgactc
     4861 acggtgcacc cacggttgtc aacgcgcacc cgcacacaag tcaaagcgga cttttcaccg
     4921 ttgtgcacaa cggcataatt gagaactacg cagcgttaaa aaacgagctt atcgaaaagg
     4981 gctttagatt caaaagcgac accgacaccg aggttattgc tcaaatgctc gaatatctct
     5041 ataacggcga tctcaaggga actgtcggtg cagctctttc tcgcttcaaa ggctcgtatg
     5101 cacttgcgat aatgtgccgt gattaccccg actgcattgt ttgcgccaag ctgtccagtc
     5161 cactgtttgc aggcatttca agcggctgcg tttatcttgc gagcgatgtt tccgccattg
     5221 cgacaaaaag tgacagaatt ttcagagtta atgacggtga aatcggtctt ttaaaaaaag
     5281 aaggtctgaa ggttttcgac cttgacggct gtcgaataag aaagagcgaa atcaagctca
     5341 aaagtgcgga acgaagctca gataagctcg gctataagca ttatatgctc aaggaaataa
     5401 tggaacagcc ggcggcgatc aggcgcactc tttcggaaat catcggcgag accggcaggc
     5461 tgaaattcaa ggggcttcgc atgactaggg cagaaatcaa tgcgctcgat cacgttgttt
     5521 tcgttgcctg cggctcggct taccatgtcg gcgcagtcgg cgcatacgcc tgcgaacggc
     5581 tctttaaacg tcattctcaa gcagttatcg catcggaatt tcgctatatg caccctcatc
     5641 ttaacgaaaa gacgcttgtt ataatcatca gccagagcgg tgaaacagca gacacgcttg
     5701 ccgccatgcg aatagctcag gaaaaaggag caaaggttat cggcatagtc aatgttgaaa
     5761 acagcacgat agctcaggag tgcaaaaacg ttgttctgac caaggccggc agggaaattg
     5821 cagttgcgac aaccaaggca tattccgccc agctcgccgt tgtttatgcc cttgcaattc
     5881 gccttgccga ggcagacgga gcaatcagcc gccgtgaagc cgcagagctt gtagaggagc
     5941 tgaaggcaat acccgataaa atcgaagcga tactttccga cgccgaaaaa atcaaaccct
     6001 ttgctgacat tgccgatcaa tcggactata tctgcttcat cggcaggggc gttgactact
     6061 gtgcggcaat ggaggcgtcg cttaagctca aagagatttc atatattccg tgcgaagctt
     6121 atgccgcagg agaattgaag cacggaacta tttccttaat agacaggaac agtctgaccg
     6181 ttgcgctgtg ctgcgtcgat gagctgaaaa gtaaaactat atcaaatatt aacgaggtca
     6241 aggcgagaaa cggcagaaca gtaaccgttt caaccgaagg cggcgatata accgtacccg
     6301 atgcgcccga ccttttcatg ccgatgcttg aggtcattcc gatgcagctt ttggcatacc
     6361 ataccgcact caaaaggtgc tgtccaatcg acaagcctag gaaccttgca aagagtgtga
     6421 cggtggagtg ataaattgac agtgagctta tgtttgtggt cagtgggtgc gacccctacg
     6481 aaatgtgtct ttatctaacc tcaccttagc accatgaaag cctgaaaaaa ttcatttttc
     6541 ttgtacaagt ttttttcctc ccacacagat atgccgagat aaatttctgc aactccagca
     6601 accatttaac aaaattttct caacaattat gtttttatta tactcattat acaaaataat
     6661 cacctgtgtt tattgacaaa cacccgatct cggttgtata attatagaga ataaaaatac
     6721 aatggaggca gactctatga aaaaagaacc tttgcgtttt aataaagacg ggaaatttaa
     6781 aattatgctt atcggagatc ttcatgaata ctacgacatg aagagcgaca atgctgcggc
     6841 aaaggttgac gacactctca atcttcttac gaaagccgtc agcgagctgg agcctgactt
     6901 tgttatctat ctcggcgaca atgcgagagc tgcaaccgag agtcagatga gatccatcat
     6961 cagccgtata acctaccccg tgtcggttaa tgagattccg tttgaccttg ttttcggcaa
     7021 ccatgaccgt gagtgcgacg ttccgcttaa aactcagctc aagctctatc aggagcatga
     7081 taactgctat gcattcaatg cggacgacag catttcgggc tgcggcaacc acaatataat
     7141 catcaaatcg cacgacggcg aaaaagatat gttcaacctt tggcttattg acagtcataa
     7201 tctttatgag gatcgctcaa agtcatatta cgatgttgtc cacgaggatc agcttgaatg
     7261 gtacaaaaag actgcaaagg agcttgcgga aaagaatgac ggcaagccta tcccctccct
     7321 cgttttccag cacattccgg ttcccgagga atatgagctt ctcagagaag ccaagccata
     7381 tgagagactc gacagcgttc agggacacaa gacctacagc gacaagtatt atgttctcaa
     7441 gccgcaggtt gaaggctacc tcggcgaggc ccctgctgtt ccggatttca acggcggcga
     7501 gtttgtcgca tggaaagaga caggtgacgt tctcggagca ttcttcggtc acgatcatat
     7561 gaacgacttt gtcggctttg ttgacggaat catgctcggt cagtgcaaga cagcaagctt
     7621 ccgagtttac accgacggct gccgaccggg cgtaagaatg ataactcttg acgaaaacaa
     7681 cattgaaaac gttcagacaa agatgtatca ttttaaggat ttcggactca agagtaaaag
     7741 ccttgacccg tacatgagac acgttaccga caggcaggat atgaagctga aggtttacgg
     7801 cactgcaatc ggaacggttg cggctgttac tgcggcggct gttgcggtaa ataaggtttc
     7861 aaaggcgaaa aagaagaaat aatcggacat actaagactg aggcttcggc ttcagtcttt
     7921 ttttaacggg tacaacgatg accttgggtc gatgtgggca tcgaccccta cggattgtgc
     7981 ctttatcact ccctcagtca cgacacaagg tcgtgccagc tcccccggag gaggagccca
     8041 cgctttaatc aaatttctgc ggactttttt cttgtctccc cggcagggga ggcttcggcg
     8101 ggttgtgccg aagtcggaag ggttaaaata aaactcatct agctttttaa aaacctcagt
     8161 cagcttcact gtccggctcc cctaaatggg aaccgagtaa atttctgccg atcttaaata
     8221 cataccaaat tacaactctc ggtgatgcta tgcttctgtc attcggcaac aaaataaata
     8281 aaatcggctt tgatgaggtt aattcatctc cgttcacggt cgcccttatt tcggctgatg
     8341 aatttaaaaa gcattattct caactcggta tttcaacgag ggaatacgtc tgctttgatc
     8401 tcagcgattc cggctttcgc agctcggtga cggtcaattc cgacagcttt gttttctctg
     8461 tcaaaatcgt tgaaccgagt cttagctgta ctgacgaaag cgcggcttgc atttatctga
     8521 ggaaaaatct tgtccttatt gtaaccgtga aggacgacgc aaagattctt tataatgccg
     8581 tttatgccgc acttgactat gcagagccga gtgacatttc cgttgagaaa ttcacaggtc
     8641 aaattcttca caagcttatt gaaaatgacg gcagaaagaa cgaggaaaca gaaaccgcga
     8701 taaacgagct tgaggaaacc gtcattgaaa agggtaagta caagaacgtc aacgaacaga
     8761 tactcgccta caacaagaaa cttgtatcgc tgagaaatta ctatgaacag cttgtcggta
     8821 tcgccgaacg gctttatgag aacgagaacg gcatatttga cggcgaaaaa gcatattatt
     8881 taaaaaccgc cgccgacaga tcaaaacgcc tttgctccga gataaatctt tttcgagaaa
     8941 accttgtccg cctcagagaa gcctatcaag ctcggctcga cctgaaattg aacagcacaa
     9001 tgaagcttct tactgtcata acggtcattt ttcttccgct gacgcttatc acgggctggt
     9061 acggaatgaa ctttgtcaat atgccggagc tttactctcc ctatggctat cccgccgtca
     9121 ttgccgtgag tattgtgata attttacttt gcattattat tttcagaaag aaaaaacttc
     9181 tttaaactgt tgacaatata aaaaagcggt ggtataattc gttcatatcg aaatgcaaag
     9241 atgaagagaa gtaacgcaag agatcgttct cagtgagcgg cagagagtgc gaacgccgta
     9301 aaagagcttg cgtgaataac acttcggagc ttcaagctga acgggcaact cagtaggtgc
     9361 gacgtggaca cagcgttaat gtgcatggct tattcgagcc gtaaagggac tcagtaatga
     9421 gtaatttagg tggtaccgcg gatacaaggc tattcgtcct atactaaggg acgaatggcc
     9481 ttttttgttc gtcaattacg atttttccaa aaaggagaat gatttattat gaaacacacc
     9541 gacatcaaag aaatcctgaa agacgcatcg tatatcggca ctcaggtcac cgtttgcggc
     9601 tgggtcagaa cctcaagaga ttccaagaac atggctttta tcgagcttaa cgacggcaca
     9661 tcgctcagcc atcttcaaat cgttatcgac aagtcgaagc tttccgacat ttcatcattc
     9721 atgcccggcg gaacggctct caaggtaatc ggcacagtcg ttaagggcgc agtcaacaat
     9781 gccgttgagg tcaatgcgga cgagattact ctcctcggcg aatgtccgag tgactacccg
     9841 ctccagaaaa agcgctgttc gctcgaattt ctcagaacaa ttccccatct tcgtgtaaga
     9901 accaacacgt tcaacgccgt tctcagggtt cgttcggttg ccgctgcggc tatccacagc
     9961 tttttccagc agaacaacta tgtttatatc cacactcccc tgctcaccgc ttccgactgc
    10021 gagggagcag gcgaggtatt caaggtaact actatcggtt attccgacaa gtacaaaagc
    10081 gaggaagaat actatgccga tgatttcttc ggcagaaagg caggactttc cgtttccggc
    10141 cagcttgagg gcgaggttgc cgcaatggca atgggcaaga tttacacatt cggtccctcc
    10201 ttccgtgccg aaaagagcaa cactcagcgt cacgttgccg agttctggca cgttgagcct
    10261 gaaatcgcct tcgccgagct tgacgacatt atcgaggttg ccgaagcaat gattaaattt
    10321 gttctcaagg acgttcttga annnnnnnnn nttctttgaa aaaggacttc gtgagaagct
    10381 tcagaaaatc gttgacggag attttgaaag aataacctac acaaaggcaa ttgagcttct
    10441 caaggatgca gacgtcgagt tccagtaccc gattgaatgg ggctgcgacc ttgcaacgga
    10501 gcatgaaaga tacatcaccg agcaggttta caacaagcct gtgttcgtta ctgattaccc
    10561 gaaggagatc aagtccttct atatgaagca gaaccccgac ggaaagacag ttgcagcaac
    10621 agaccttctt gttcccggtg tcggcgagat catcggcggc agtcagcgtg aggaaagcct
    10681 cgacaagctc cttgccgcaa tggaggagag aggcatgact caggacgagt acaaggatta
    10741 ccttgacctt agaaaattcg gctcggttcc tcacggcggc ttcggcctcg gatttgacag
    10801 aatcgatatg tacatgaccg gcatggcaaa catccgtgac gttcagctct acccgagaac
    10861 agtcggaaac atcagataaa tcagggagga atatattatg agaagcttac agacagtgga
    10921 cggatactcc tccgccctca cactcagaga aactcagaag gcaattaagc ttgttaagga
    10981 tacctttgca aatgagcttg ccgctgcgct caaccttgag cgtgtcagtg caccgttcat
    11041 tgttaagaaa ggcagcggta tcaacgacga tctcaacggt gttgagagaa aggtcgagtt
    11101 tgatattaaa gaaattgaag gcactgcgga aatcgtccag tcgcttgcga agtggaagag
    11161 aaccgctctc aaggcttacg gctacggcgt tgacgagggt ctttacacca acatgaacgc
    11221 aatccgccgc gacgacgact gcgacaatgt tcattcgatt ttcgttgacc agtgggactg
    11281 ggaacgtgtc atctcaaagg aagaaagaaa cgttgaattt ctcaaagata tcgtaaagaa
    11341 aatcgtcggc gcaatcgaca acactcagaa gatcgctcac aaggctttcc cgcagctcag
    11401 gaaggagctt gcaacggatg ttttcttcat cacaactcag gagcttgagg atatgtaccc
    11461 cgacaagact ccgaaggagc gcgaggacat catcacaaag gaaaagggcg tcgttttcat
    11521 aatgcagata ggtgacctgc tcaaaagcgg catcaagcac gacggcagag cgcccgacta
    11581 tgacga
//
LOCUS       DAWBSW010000047        11528 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1211_length_11528_cov_2.798832, whole genome shotgun sequence.
ACCESSION   DAWBSW010000047 DAWBSW010000000
VERSION     DAWBSW010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_11528_cov_2.798832"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2859..2868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2939 a         2888 c         2468 g         3223 t
ORIGIN      
        1 cccctgcttc tcttgtaaat attattgccg aagcaggtgc tgtgttttcc gttatggtga
       61 caaacatgtc ccctttgctg aacgctcatg agcaaagcgt cgccgccgca atcaaggtca
      121 agcgacataa ggcgctgcgc attgcccttc tcctcgccga atttataaac cttgtctgtt
      181 tgagcgtcat agtaatatgc cctgcgtgtt ttgagcgtat aggcaaaggc ctccttattc
      241 atatatccga gcatgagcac ctttcccgat ccgtattcta ccgttatcac gggcatggtg
      301 gttttgtttt cccaaagctt gtaaagatct gtcattttat ttcctccgtt attaaaatcc
      361 aaaaaagctc atctgcgtgc tctgcggcaa accttcaagg gcgccgcacg catcaagcgt
      421 ttccataacc gacttgctga ttccggctcg ctgctgaaga tcgtcacggg acatatactc
      481 tccctcgccg tcatctcttg ctttcataag agcaacagcc gcagactcgc cggtgccggc
      541 catggacatg aacggaagtc ttatttttcc gtcctccaat ttataaaccg ttgcatgaga
      601 tttgtaaagg tcaatcggca ggaactcaac acctctcgcc atcatttcgt taacaacctg
      661 cattgcaaca tacatatttt cttcctttgc cgttgcctcg tgacctttat tcaggatgga
      721 gttcatgagc tgtttaacgg ctcccctacc ctccatgatt gcgactgtat caaggtcttc
      781 accacgaacc gtcatatagg tcgcatagta ttcagtcggc ttatatacct tgtaccatgc
      841 gaggcgcatt gcggcactga catatgccgc ggcgtgagcc ttcgggaaca tatacttgat
      901 cttcatgcag gaatcaatgt accaatcggg aacattatgc tccttcatgg ctttaatgtg
      961 cgcttccgtc agaagcttcg ttgcattacc cttacggaca atctccatta tcttaaatgc
     1021 catatcgggc tcaagtccct tgtgcataag gtaaaccatg atgttatcac gagttcctat
     1081 aacctcggaa atcgtacatg gaccgttttt tataagctcc tgagcgttac cgagccaaac
     1141 gtccgtgccg tgggaaagtc ccgaaatctg aagcatatcg gaaaaattct ttggcttagc
     1201 ttccataagc attcctctaa cgaatggagt gccaagctcg ggaagcgaaa gcgtaccggt
     1261 ttcgcagcca atctcctcca ctgttactcc gagcggttcg ggggaggtca aaagctcata
     1321 aagcttcggg tcacagatat caacatcaaa aacattgatg cccgtcgaat cctcaagatg
     1381 acggtaaagc gtcggaacat cgtgaccgag gttatccagc ttgagaatag tatcatgaag
     1441 cgaatggaag tcgaagtggg tcgttctcag tccgcccgaa acgtcgtccg ccgggtgctg
     1501 aataggtgtg aagtcctcgg cgtcattgct tgccggaaca acgaccattc cgccggggtg
     1561 ctgaccggtc gttctcttaa ctcccgtaca gccctttgtc agtctgtcct cctcggcacg
     1621 gtttaccgtt atacccttga cctcaagcca ttttttaaca aagccgtaag cagtcttgtc
     1681 ggcaatagtg ccgattgtgc cggccttaaa tgtatggtca attccgaaaa gctcctcggt
     1741 gtatcggtga gcatagaact gatattcacc gctgaagtta aggtcaatat cgggctgttt
     1801 atctcccttg aatccaagga atgtttcaaa cggaatatcg tgaccgtcac ggtgcatcgg
     1861 aattccgcag ttcgggcaat ccttcggcgg caggtcataa cccgaaccga cagaaccgtc
     1921 catgaaaaat tccgagtgct tacatttttt acatatatag tgcggcggca gagggttaac
     1981 ctcggaaata cctgccgcat gggcaacgaa cgacgagccg acagaacctc tcgagccgac
     2041 ataataaccg tgctcctcag aattctgaac aagcttttgt gcaatcatat aaagaactcc
     2101 gaatccgttc gaaatgattg atttcagctc tttttcaagt cgttttgcaa cattttcggg
     2161 cacgggatcg ccataatcct tcttagttct ctcccagcaa atacgggtaa ggtcctcatc
     2221 cgctccttca aggctcggcg gaaatgttcc tctcgggaag ggtgtcatgc cgctttcgca
     2281 aaggtcggcg attttattcg gattggtgat aacgacctct ttcgccgtct cctcaccgag
     2341 atatgcaaac tcggcaagca tttcttccgt agttctgaaa tagagcggag cctgctgaga
     2401 agcgtcggaa aatcccattg aggtcatgag aatttcgcga aaaacagcat tgccggggtc
     2461 aatgaaatgg acatcgcaag tggcaacaac cattttgccg agcttgtccg ctatatgaat
     2521 tatctgtctg tcaacatttt caagatcctc aaccgtctta aagcgctcat aacgctcgtc
     2581 ctgactgcga agcataaaag cgttgttgcc attgggttga atttcaagat agtcatagaa
     2641 agaagcgatt ctgagaatat cctcatcgct tttttggtcg acaagcgcac ggtaaagctc
     2701 gcccgcttca caggcacttc cgataataag accctcgcgg aactgcataa gtctgtgacg
     2761 gggaattctc ggtctgcgct tgaaaaattt aaggtttgaa tccgtaataa gcttatagag
     2821 atttttaagc ccctgagtat tttttgcaat tataattann nnnnnnnnta attatatgat
     2881 aagtcttttc ggcacggact ttttcgatat attcggcagg tatctcatcg tcaacgccga
     2941 agccgtaatc atcatagaaa gcatcatcgt caacgaggta gccctccatg ccgtaaataa
     3001 tttttattcc tgccttggcg gcagtattgc aagcctcggg gaaagcctgc acaactccgt
     3061 gatcggtaat tgcaatagcc ttgtgacccc atgctatcgc acggctgaca agctcctttg
     3121 cgctcgtcat accgtccatt gccgacatat ttgtatgcag gtgcagctcc actcgctttt
     3181 cgggagcgtt atcctgcttc tcgatttttt caatttccgt gacggctctt gcattgatga
     3241 gataggtgtg catataggtg tccatattta ttgagcctcg gacaagcacg gttttgccgt
     3301 ccttgagatt ttccaccaag tcttcgcact ttttcttctc accaaaaacc ttgaccggat
     3361 atgatgaagt gtagtcggtt atattgaaat tgattatgct gtagcggccg tcctttgact
     3421 ctctcgtttc gagggagaaa acatcgcccc aaacaacaac ctcgccgtcc tccggcaggc
     3481 actctttgat aggcttcggc ttacccttga caagcgtgcc gaaaagaggc ttagcatttg
     3541 taattgaaat cggtaaatcg tcgtaggtct tttctttagg cttcttctga gccggtacct
     3601 ttgaagcggc aacgggattc gccatcggcg aagcaactgc aagcttttga ttttcctttt
     3661 tctgaagctc ttcaagtgct tttatgtcgt cctgagccga agcattgccg acaaattcaa
     3721 ccgctatcag tctttcaaag cgtcggtcaa tcgtttgacg aatgaattta tcgcatccga
     3781 gattaacaag cacgtccttg ccgttcgccg caagctcaac tgtaagcctg tcaccgttga
     3841 ggttgtaggt tgcgccctca agaattgttc ttgacgcagg aaaatgctca tagatttcct
     3901 tgacgaagtt cgtcatatag tttatatcaa atgtctcggg agggaaatgt attccgaggt
     3961 taagccgatg caaattcatc acacggataa gctctgctct cgcctttttg attactgccg
     4021 agtcaatggg cgcaggaaaa ctgacgagca tactgacact gcgttcagaa aggctcgcct
     4081 caatgctctc aaccgttgcg gaatcgaaat acgccgcaat gccctgatct atgtactcgc
     4141 cgaaaaggct gagaaaactg acttctttca aaattacact cccaaattat ttaaggtgtg
     4201 tgccgcaatc tatgcgttat tgccttaaat cttatctatt tcagccagca gctcgctgac
     4261 aatatcttcc tctttaatct ttcttataat ttcgcctttt ttgataagca cagcgcatcc
     4321 gtcaccgccc gctataccga tatcggcttc cttcgcctcg ccggggccgt tgacaacaca
     4381 gcccattaca gctaccttga gcggcttacg gcaatctctg agcgcatttt caacctcgcc
     4441 tgcgaccttg ataagatcaa tctttgttct gccgcaggtc gggcaggaaa ctatctgaac
     4501 gcagtcgctt ctcaaatcaa gagccttgag aatatcaagt cctgcataga cctccttgac
     4561 ggggtcggca gtcagcgaaa cccgaacggt atcgccgatt ccgtgcatga gcagacttcc
     4621 gattcctgca ctcgatttaa taagacccat gcgctccgtg cccgcctcgg ttacgccgag
     4681 atgaagcgga taatcgcatc tttcggcaac ggtttcatat gcctttatca tattgccgac
     4741 atttgacgac ttaattgaaa tcacaatgtc atcaaagtca aatttttcaa gcaatgaagc
     4801 atgatacatt gcgctttccg ccatagcttc cggggtcggc gcaccgtatt ttgcaagaat
     4861 atccttttcg accgagcctg agtttacgcc gatacgaata ggaatatttc tcgaacggca
     4921 cgcttcgaca acctttttga ccctgtcatc cgacccgatg ttgcccggat ttatgcgcac
     4981 cttgtcaact cccgccgcaa ccgcttcgac agcaagacga tagtcaaaat gaatatccgc
     5041 aacaataggc acgctaaccg cttccttgag cgcagggatt agcttcactg cgtccatatc
     5101 gggaattgcg cctcggataa tctgacagcc cgctttttca agctcgaccg cctgcttaac
     5161 gctcgcttcg atatcgtgag ccggtttgtt gagcattgac tgcaccgtta tcggtgcgtc
     5221 gccgccgata tatgtgtttc cgatttttat ttttttcgtt tttcttcgtt ccattttaat
     5281 acctcggttc ggccaaaatg tttttctgtt tttctttata tcctcacttc acaatcagcg
     5341 aatatatatc cttaaacgtg attgccgcca taaagagcag gagaataacc ataccgatag
     5401 cgtgaacaag ggattcgtac ttttgcttta tcggctttct tctgacaagc tcaatgagca
     5461 ggaagaaaag acgtccgccg tcaagagcgg gaatcggcag aagattgaac acgccgatat
     5521 ttattgtaat aagagccatt atgctgagaa gtcctgtata gtcagccgtc ttgaccgact
     5581 cctgtgccgc atcggaaaca tagtccacaa cgccgacggg gcccgaaaca tccttaacgg
     5641 aatacttgcc cgtaacaagg tcaaacaagc tcagccagac cattctgaaa atactgacgg
     5701 attccttaaa tgtatcctta aaaaccgtgc cggcagtctt gtcaacaccg accataacta
     5761 tttttgaata gggcatttca acgtcattga tttcaacctt cttgccgtca cgctcaacga
     5821 gaagatctgc ggttgagcct tcgtcacgac tgagaagata gggaacatcg gtataataat
     5881 aaactctctt gccgtctatt tttattattt tgtcttttgc ctttagtccg ttatactctg
     5941 cgacctgctt gccgttgtca ttgaagaaat tgacatagtt cgttccggaa agattgtccg
     6001 aagcaccgag cattatcgca attataataa gtccgagaat tatatttacc atagcgccgg
     6061 ctacaacaat tattattctc tgccaacagg gcttgttgtt aaaggcacgt tcatccgtgc
     6121 tgtcttcgtc ctcgccctcc atgctgacaa agcccccgat tggaagcaaa cgccatgaat
     6181 attgagtctc gccctttttc tttttcagca atgtaggccc catgccgaga gagaattcat
     6241 ttacctttac gccgaaaatc tttgcaaaag tgaagtgtcc tgcctcgtgc accatgatta
     6301 ttatgccgaa gaatattatc gcaagcagaa tcgtccattt gctcgccagt gcagataaaa
     6361 atcctatttt aatcgcctct ttcgtgagcc gagcgacctc agcccattat tttttctttc
     6421 acataacccc tcgcccagtt atacatatcc aaaacgtcgc cgagtttata tgtatcaaag
     6481 cgctgagcgt tctcaacggt ctctttgacc agttcctcaa tctgcaaaaa tttaatcttg
     6541 ccctgtctga aagcaaggtt tgcttcctcg tttgccgcat ttgcacaggc agggaaaagt
     6601 ccgccgtcgt tgattgcctt tcggcagacg tttatcaagc cgaaggtttc gtaatccggc
     6661 ttatcaaaag taagtgtgcc gtaatccgtc agcgaaagct ccttgacggg tgacggaatt
     6721 ctgttcggat aagtcagcgc atactgaatc gggattcgca tatcgggaac accgagctgg
     6781 gcaataacgg aattatcgca gaactcaaca agcgagtgaa taacgctttg acggtgaaca
     6841 acaatatcaa tatcctttgg atccatcgaa aaaagccaaa ctgcttcaat aagctcaagc
     6901 cctttgttca tcattgaagc ggaatcaatc gtaatttttg cacccatgct ccagttcggg
     6961 tggttgagcg cctctttgac cgtaacatca acaagctcgt cacgcttttt tccgaagaat
     7021 gggccgcccg aagctgtaag aatgagcttt tttatctctt tcttgtcccg acaaccctgc
     7081 attgactgaa aaatcgccga atgttcgctg tcaacgggaa gaatgttgac gcccttttcc
     7141 tttgcaagcc ccataacgag ctgaccgccg gcaacaagcg tttccttgtt ggcaagtgca
     7201 agctcactgc ctgcgttaat tgcagctatt gtcggctcaa gtcctgccat gccgacaatg
     7261 ctgttgagca catagtccgc tccgcttgca gcacattcgc aaacgccctc catgcccgaa
     7321 agcaccttag ttcgagtgtc tgcaactctg attttaagct ctgccgccgc tttttcatcc
     7381 accattgcgg caagctcggg cttaaatttt ctgatttgct tttcaatcgt atcaacgctt
     7441 atgcttgccg ttaaagcttt gacttcgtag ccctgcattt cgatgacttc aagagcctgt
     7501 gttccgattg agcctgttga gccgagaacg gctacggttt tactcatagt atcaccttaa
     7561 attttatttt taaaatgtct cgattatgct gagtattccg tacatgatcg gaaaaacaaa
     7621 cagcgtactg tcaaatctgt ccataattcc gccgtgaccg ggaatcagct ttccgtaatc
     7681 cttcacgccg tagtttctct taataaccga ggcggaaagg tcaccgaaaa tgctgataac
     7741 ggaaagcacg agcgaaatcc gaattactgc ccaccaatcc caaaggaaaa agcttccgtc
     7801 cgggaaaaat ttctttgcaa acacaaaata taatccgaca ttcagcgctg cagtgaggac
     7861 aactccgccg attgctccct cccaggtttt tttcgggctg acaacagggg tcatcttatg
     7921 cttgccgaag aatacgcccg tgaaataggc gaacatatcg gtaagccatg cgcagaaaag
     7981 cgtaaacaat atgagataaa ccacgttctc ctgagtgtat ctttcgtcga taagctgaag
     8041 atcgtttatt cttacccaac agccgagcga ctcgggaacg gcgattgagc aaagaacaga
     8101 aattgcgacc tgctcaaatt taacctcacc gtgccactta accatcataa tgagcatggc
     8161 gataatatat acggcaagag ccgcaaacgg tgtaacatgg attttttcaa ggaaatgata
     8221 tctgatttca atgggaatat atgcaccgac tataatcgac ggaatcgcaa gcggaagctt
     8281 gaccttcgca acatggttga gctcataaac ggcaacgccg caaataaatg ccatgagtat
     8341 cggcaatgcc gccgtgttca tgaaaaacat cacggcaata atgatgaggg aatatgaaac
     8401 tcccgaaata attctggttt tcatgtggtt tatcctttca aataaggtac tgcatcgaac
     8461 aaatgctttt tcagcaaact tttgagattg aggaaaattt tttcacaggc ggttttgcag
     8521 gtcgggtgct gtgctgttgc actgccctga gcgaaaaatc ggttgtggag ttttacctct
     8581 gaataagttt gtaagaaaat tgttcacagt gctttttgca gcttgtgagc tgtacaaagg
     8641 tactgccaca agcgaaaaaa gtgctgtggg cgattttata caaacttaga cgccgccgaa
     8701 acgacggttg cgcttattat aatcgtctat tgccttgtcg agatcggcag gagtgaagtc
     8761 cggccaaaga atgtcggaaa accaaagctc ggaatatgcc gactgccagg taagaaagtt
     8821 tgagagacgg tattcgccgc tgggtcgaat gatcaaatca gggtcgcttt gtcctgcggt
     8881 atagagatga gaggaaatat cgtcctcggt gatgtcggcc ggatcaagct cgccggcctt
     8941 gattttttcg ccgagctgtt tgacagcatc gacaatctca agcctgccgc cgtaattgat
     9001 ggcgatattg acatcgtatt tttcggcgcc tcttgactct tcctcggagg tgtcaaagag
     9061 attgagcaga tcctcgggaa taccctcacg gtgtccgatg aagtgcattc tcatgccctt
     9121 ttgaagattt tcggcttttc tgtcctcggc ctcgtgcata tattcacgga aaagatccat
     9181 gagtgacgaa acctcctcgg gaggtcgctt ccaattctcg gttgagaacg cataaaaagt
     9241 aatgctttgt acacctattt ctcctgcata atcgcaaatc tgtttgaaca cctttgcacc
     9301 ggctttatga ccctcggaac gcggcaagcc tcgctgcttt gcccaacggc cgttgccgtc
     9361 catgattata ccgatgtgtg tcggaacttt tctttcttcc ataacaggtt ctcctctatt
     9421 gacaacaggc agaccgtgaa agcctgcctg ttaaaataat caaagcgtac gcaattttat
     9481 attgacataa cttccttttc ttttgttgaa gcggctgcgt cgatctcctt gatgaattca
     9541 tcggtaatct tctgaagctc gtcctcgccg ttcttgaggt catcctcggt gatttccgac
     9601 gccttctgca ttttcttaat cttgtcgata gcgtcacggc ggatcgaacg gatagcaacc
     9661 tttgaatcct ctgccatttt cttgatttct ttaacgatct ccttacgtct gtcctctgtg
     9721 agctgaggga aggtaaggcg aataacggat ccgtcattct gcggattgat gccgatatcg
     9781 gaagcttgaa ttgccttgta aatcggggta agcgtggact tatcccacgg ctgaataaca
     9841 agaatgcgag cctctgaaac cgagattgca gccatctgat tgattggcgt cggcactccg
     9901 tagtaatcaa caagaacctt gtcaagtacg cccggatttg cgcggcctgc tctgattgaa
     9961 gcatagtcct tgccgagaac agaaaccgtc tttgtcattt tttctctggt attgctgtaa
    10021 acttctttca tgaaaattct ccttacttga tttatttata tttttattct ttaacgagtg
    10081 tgcctatatt ttcgccttta acggctctga taatattctc gggatcttca aggttgaaaa
    10141 cgagaattgc tattgaatta tccctgcaaa gcgaagcagc cgtgctgtcc attaccttga
    10201 gtcccttggc aagaacctcg gaatgagtga gcgtatcata cttgaccgca tcgctgaact
    10261 tgttcgggtc cttatcaaaa acgccgtcaa cattagttgc cttgaatata acatcggctt
    10321 cgatttcagc cgctctgagc gccgccgttg tatcggttga gaagaacggg cagcctgtgc
    10381 cgcatccgaa gataacaacc tcgccttcgt tgagatgagc gaccgcgtca cgggcaacat
    10441 actgctctgc aatcggctgc atacctaccg ccgacataac tctcgcctta actccgagct
    10501 gctcgagagc ctcacaaaga gcaagcgaat tcataaccgt tgcaagcata cccatgttat
    10561 cggctcttgt gcggttcatt gtgccgctgc ttcttccgcg ccagaagttt ccgccgccga
    10621 caacgagacc aatttgaacc cccatatcgg caaccttttt aacgtatgta caaacattgg
    10681 tcaccatgtc aaaatcaatt ccgtgaccct gctcgccggc aagaacctca ccgctgagct
    10741 tcagcagaat tcttttatac atctgaatac ccccactgcc ctaatatatc gtatatattt
    10801 tactttaaaa cgacggttat gtaaagtgaa aaatgatatt tttcatgaaa aattcaaaaa
    10861 tatttttaca aaaacaaaaa cggatcgttt ccacgacccg tcgaattact atttaattag
    10921 ccaaacaaac cgaaagcaat ggttccgact atcaatcctg caatcaaaac acccaaggca
    10981 atcatcggaa gcgacttttt gattttgata tccattatct ccgcaacaag tgcgcctgtc
    11041 caaccgcctg tacccggcaa cggaatagca acaagaacca aaagtcccca tatgccgtat
    11101 ttctcaattg tgtgtctgtg cttttcggct tttttctcca ttttgtggac aattttaccg
    11161 agacctttgg attttttcat cagctcgaaa atctttttca agaaaagtaa gatgaaagga
    11221 atcggaagca tattgccgag atagcaaatg atgaacgcct tcataacgtc catattcaaa
    11281 agtcttgccg ctatcaatcc gcctctgagc tccaatatcg ggagtacaga gatgatgaaa
    11341 acagtaagct catttggaat tttgctttga aaaaaagcca caatggactc tacaagttca
    11401 ttcgtcattt ttttactccg ttataatttt tttgttcaga agataattat aagccttgga
    11461 aagaatgttt ctgtcgttct caaagcgaag cttattaaga gctttttcat atccgagcca
    11521 aatataat
//
LOCUS       DAWBSW010000048        11515 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1212_length_11515_cov_2.680716, whole genome shotgun sequence.
ACCESSION   DAWBSW010000048 DAWBSW010000000
VERSION     DAWBSW010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1212_length_11515_cov_2.680716"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6858..6867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3316 a         2551 c         2512 g         3126 t
ORIGIN      
        1 gaaaatcccg acatcttctc cgttaagctt gttcttgcag gaattttctc ttatcccgac
       61 ggtcttgcga gagagattat tcatattcag acctataagg agcttttccc gtacgcaaga
      121 gcttttatca cgacgataac cgccaatgct ggaattcagc cgataatgat acccgatatg
      181 gatatcgaaa acagagagat ttaccgtttt gataatccga acaagcagaa ctgaacaaat
      241 taaccgcatt tgatttttca tctgcggttt ttcttttgtc aatttaatta aactgcacaa
      301 ataaaattga caatttattt ctttcgtgtt aaaattaaat gaaattaaat ccataggaag
      361 gtgtatcttt tgaaaacaac attattttca cgcattgctt ataagctgat ggttcttatt
      421 attgccatct catatacttt gactccgtat gtcggtccgt caaatgagga tccgatcctt
      481 acaaacgaaa agacaggcgc taatctcacg gctgttatgt gggccgatcc gcagttctca
      541 aactatcttg ttgaaagaac aaggtatttt gacgccgctt gcgaggacgt tacatacaat
      601 atcgacggca aggttgacgc aattgttaac gccggcgata tagcggaaaa cggacttctc
      661 tgcgaatatc agtatatttc cgagaagctt gcaggcgcaa gaacaaactg cttccttaat
      721 gcagtcggaa accatgatgt aagactcaag gcaagctaca aaaaaacggt cagaaacttt
      781 acaggcttta ccaatgagct taacaagtcc gtgggcagtg acttcaccat tgattcgctt
      841 cattattcat atgagatcaa cggctacaga ttcattgttc tcggaacaga cagaacagag
      901 tttgaggagt cctatatcag cgaggcacag ctttgctggc ttgacgctca gcttatcgag
      961 gcttcaaaga ccggcaagcc catcttcgtt atgatccatc agccgctcaa gcagacacac
     1021 ggtcttcctg atacatggaa cagtccgatt gattcggcag gctcggttgg tgctcagtcg
     1081 gattcgctta agtatttcct taataagtat gataatgttt tccttattac gggtcacctt
     1141 catacaggca tcggacagta cacttacgag aagatcggca acttccattc gatcaatctc
     1201 ccgtcgctta ctattgataa caaggacggc gagtgcaacg ataacggcat aggtttcgta
     1261 atggaggttt atgacagtca cgttctcttc agagcgagaa actttgccaa gggtctttat
     1321 atgcccgaat atgacattga tatcccggta gaataatata aatatttatc aggagggcgt
     1381 ttttatggaa atcgtcagaa aagaatattc ctatccttcg gtcacaggtg aggccgacat
     1441 tttcgcaaga agctgggcac ctgccgacgg cagaattaag gctgttgttc aaggcgttca
     1501 cggcatggct gaattcggtg aaagatatga ggagtttgca actgctcttt gcaatgccgg
     1561 cttcgctttc attatgaacg accatatcgg tcacggaaaa tcagtcgcac cgaacggaac
     1621 taaaggctat ttcggcggcg agaaaaacgc tttcggcaag ggctttgttg atgacgctca
     1681 tcagctcact cttattgcta aagacgagtt caaaaagcct gtaattatct tcggccacag
     1741 tatgggctcg ttcgtcacaa gaagctatat tacaaaatat gccgacgata ttgcagcagc
     1801 cgtgatctgc ggcacaagcg gaaaaaatcc gggtgcaggc gcaggaattc ttcttgcttc
     1861 actcatttca aaggtcaaag gtgaaaagca cccgagtaag ctgattgata aaattgcttt
     1921 cggtacatat aacaagcgca cagagggcag gaccgctttt gactggctct caagaaacaa
     1981 ggaaaacgtt gactggtata ttaacaatga cgattgcggt ttcctgttca cggcatcggg
     2041 ctacaagaat atgtttgaac ttcttgactc cgtttctacc gacggctggt acagaaaggt
     2101 tccgaaggac ctcagcattc ttcttattgc gggcgaagag gatccggtcg gcgcttattc
     2161 aaagggagtt aacgaagtct ttgaaaagct taaagcaaca ggccacagca acgttcaatg
     2221 caagctttat aaagatgaca gacacgagat tctcaatgag cttgacagag aacacgtctt
     2281 tgatgatgtt atcggcttct gcgacagtgt tattaaataa aggagcaata aaatgctgac
     2341 ggtattaaac tttgtaaaat cgtttttcta tagggctttt tccgttttta ttgctttcat
     2401 aatgactttc agcaacggcc ttaacagctt cttcaacggc aatatttaca aatatgaatc
     2461 caattccaag gttgtcggcc ttgaaactct cgaacgttct cagggtgtta caacggacgg
     2521 agaatactgg tacttcagcg gaaaaacttc acttgttaaa ataggctttg acaatcagac
     2581 tgtttacgct tgtaattata aagccttgac cgatgaaatt gttgaaaaat acggctcaaa
     2641 gcacatcggc ggaatcagct attataacgg ttttatctat gcttcccttg aggacagcaa
     2701 gatctggtca catcccgtta tcgctcttta tgatgcgcaa acgcttgagt acaccggcca
     2761 ggcacatgag ttgcccaaca gcattcttac aagaggcatt ccctgggtat cggttgatgc
     2821 ggccaacggt ctgctttatt cctcatacag caagacggca gaggttattt actgctttga
     2881 tcttataact tttgattatg tcggtgagct gaagctttcg tccccgataa atgcgattca
     2941 gggcggcgag gtttacgaag gaaagcttta tgtcggttca aatgacgata ccagagctgt
     3001 ttactcgatt gatgtcaaga caggcagggt cgaaaagctc tttgacagaa tcatgtaccg
     3061 ttggaaatgg attgacaatt tcggcggaga aggggaggat gtcaccgttt accctatgaa
     3121 tgacggaacg gttattcatg ctcttgatgt cggcgcatta ttcattgatt ccaacctcag
     3181 gcattacaaa tggaatgttt gaataaaaat cgtataaatt acggcacgat gggaaaataa
     3241 aatctcaccg tgccgattta tttaatttgt ctatttattt ttttcgaaaa gtatgttatt
     3301 ataaattatc aattttgaaa ggggatcatc atggttcata caagaaagat aatatctgtt
     3361 ttacttattg tcactctgtt tgcaggctgt atcggtctga gtgttaacgc agcggacagc
     3421 ttgctcctgc cggactacac aatgactgac gccgaatggg ataagtattg gagcacagtt
     3481 gagggcgaca atactcaaat tgcgcttacc cccggtgcag acgcttcgga gcttaatttt
     3541 aactggcatt ccgaaaggaa agcagcggtt cccatggtta agattgcaga caacgagaag
     3601 atggaaaatg caaagacctt ctacggatac tgcactcttg ccgacaacgg ccagcaaacc
     3661 aacagagtta ccgcaaaagg ccttgaggag aatacaaaat actattattc atattctctc
     3721 ggcaaggata attggagcga accgaccttc tacataaccc gttcaaccga ttcatttaag
     3781 gctctgctcg tcggtgatat ccagtgctct gcagtggacg acggaacagg atacaaggac
     3841 gctacaaact ggaatacaac gctcaacacg gctttgaaaa agtatcccga tacaagcttc
     3901 atcatttcct gcggcgacca gactcagacc ggtacaagtg ccgttgaatg ggcggctacg
     3961 ctttcgccaa aggcaatgcg aaatctcccg atggcaacga ctatcggcaa ccatgaccat
     4021 aaaggttcaa cctatcagca ctatgtaaac aatccgaatt ctcagcttgt ttccacaagc
     4081 aaaacaggca caccttactg gttcagatac ggcgacgttc tctttgttgt tttcaacacg
     4141 acaaacgtaa atgtatttga aagccacgct cttgccgaaa gagctatact cgctaacccc
     4201 gacgcaaagt ggagagtagc tgttttccac catgatatat acggaacggg tcaccatgcg
     4261 attgacaacg ataactatat gcttcagggc gtttattcgg ctattttgga taagtttgag
     4321 tttgacctcg cctttgacgg acatgagcac tactacggtc gttcctacaa tatgcttaac
     4381 aacgaaaagg ttgaccttga ctacacgtca aataaggtaa cggatcccga cggaacactt
     4441 tatattacga ccgcttcggc atccggcaag aaccgtgttt acgatgagcc ttatcatcac
     4501 tcatggataa actacagcta catgagccca gagcttatct actgtgaggt tgagttcacc
     4561 gagtcaacat ttaatctcaa aacatacacc gttgagggtg acaagctcat tgacgaatat
     4621 actatcgaaa agacagattt tacatattcg gatattgacg cttcacaaac acttttcagc
     4681 accgacgcac ttaacagagt tctcaagcac tttatgggta aatactatgt tatatttgag
     4741 gtgtttgaca aggttgtaag atatctttgg aatttgattt tctcaattat aaaataaggt
     4801 tcttttattg acggtttaat taaattatgt attgaaaaat aaagaacagg cagtctgaat
     4861 caatcggact gcctgctttt tatactgccg caaaaatatt caaatttctt atattagggt
     4921 cgtttattga gtcattgacc gagtatgcgt gcttttcaag gtcgccgcaa attgcccttg
     4981 tcatgttgtt ttcaatcaaa tatccgatta tcatacctgc gatatcctca attactccaa
     5041 tcttgacctc ggcggtctcg ctgtcgtttt cgcttgtcag cagatattca aaagagtctg
     5101 aaaaagtcga aaaatgttcg agattccgca ttgcgcgaaa actccattta taatacggca
     5161 tatacttttt attgagcaga aaaaccgttt tcatcgcggc tttgacaaat tctatcacgg
     5221 caagctgagc cgctccggtt tcaccgtgcg aaaggcatcg gctgtagtta tactgacccg
     5281 attgcgccat aagaagcaaa tttcccgcaa gccttttaag cctgatattc tcgggcatat
     5341 gcagtattct gttgcgaatt tcagtgaatt caccgtaatt atcaaaaaat atttcaccgt
     5401 tcgttgcttc aaaaagatag ctctcggaaa ccgtcagcca atcacgaacg tttaaattac
     5461 cgtctgaaca gccgatttta ttcaaataaa aatcagaaat acgtataact ccgcttcttc
     5521 ttccgccgac agggcttaca aggcttcttt taagtcccat aaattccttc ggaagcttgg
     5581 catatgctct ttcaagcaga aattccgttc gcctgtcaat cacatcttca tcgggaagga
     5641 aaatacaaaa tcccggctca aaatcgtgat ccctcgatgt ttcgtcgtca aagccgtagc
     5701 agtccgagcc gcttccgacc aaaccgacgc aaagataggg gagtatttcc agaaaatcat
     5761 tattcagcat cggtaaaccg tattcattga aataagcctt agaaatttca attcctttca
     5821 taaatacacc tcgctctgtc gctcagttct ttttgataaa gaaaacgtcc gaaatatccg
     5881 aatgtcggag cgcatttttc acagacgaaa gcgtaataac cgtttctcgg caaataatcc
     5941 ctgttaagaa gctcataagc tttttcgata tactcatcga ttatcttttc actttgctcc
     6001 gagccgagct tcgcctcgtt caaatcgcat aaattaagat aggtaatcgc aatctccaat
     6061 tcgccgttct tgttattcga cataacattt atcgctttat tataatattc ctccgcttcg
     6121 tcgaaccgct tgaggtcggt caaagtcagg ccgagattgt tataaagacc gccgagacgc
     6181 ggatcatcgg actgtaattc cttttcatat atttcttttg ctttttcaaa aagctctatt
     6241 gattcctcgg gcatggcaaa ggccttgtaa accgttgcgg cgttggtaaa agtcgtacct
     6301 gcaacaacgg cgctgccgag ctttaattca tcaattaagt ccaaagcttt tcttacgctt
     6361 tcaagtgcgt tttctttacg gttcgtctta cgataaagac ccatcagctc gttttcaacc
     6421 gtaagcattc cgtgcttgtc tctgccgtcc tttgcttcgt cgagccagta gagaagatgc
     6481 ctttccgccg agtcataatc gttcctgcca aggtgctcgt cgagcttttc aagcaccctc
     6541 tgagtcggaa tcgggcggac aggcggctgc tttttgaact gatcggtgca gaatgggcag
     6601 ataggatcgg tataatcggt aaaatccatt ctttcctttt ccataatttc actcccttgt
     6661 gtttactttt ctgccattat actatatttt gtgcaaaaag tgaagtgaaa gtttgtatat
     6721 atttttattt ctttcttgtt gtaaaccata tcatgttgtg gtagaatact ttggaacgat
     6781 aaaatatgct ttcggaggtg ctgtgatgcg tattgacgga cttcaaaaaa tgaccctgtt
     6841 ggatttcccg ggcaaagnnn nnnnnnngtg taatttccgc tgtcctttct gccacaacgc
     6901 cctgcttgta accgaactgc ccgaaaagcc cgattacacg gttgatgaaa ttctttcttt
     6961 ccttaaaaaa agagccggac ttcttgacgg agttgcgata acgggcggcg aaccgctgat
     7021 gaatcccgaa ataggtgatt ttattaaaga gattcgtaac ctcggatatg ccgtgaaatt
     7081 agacacaaac ggcagctatc ccgacaggct caaaacaatt gttaatgaag gtcttgtcga
     7141 ttatgtcgca atggacatta agaactgtaa ggataaatat gccgaaacaa taggcttgaa
     7201 aagctatgat ttgtcgaaaa tagaagaaag cgtcgaattt ctcaaaagcg gcaaggttga
     7261 ctacgaattc agaaccacga tagttaaaga atttcatagc gtagatgata ttcgtaaagc
     7321 cgcagactgg ataaagggtg cagaaagata ttttcttcaa aattttgtcg attcgggcaa
     7381 tcttattgac agcagtgtaa ccggagtaga caagcaaaca atgctcgata tgcagaaagc
     7441 ggcgacagaa tttgtgccgc aaactaaaat cagaggaatt taaggcggca acacatgtat
     7501 tattgcgcac gccttaattg tgccgcattg cctttataca agatattgta aaaaaggtgt
     7561 tgacagcaca caagatatat gatataattt cgatcgtaac actaaataaa ggagacagag
     7621 gtatgtataa tgttgtaaaa cgtgacggca aggttgtcgg ctttgacctt cacaaaatca
     7681 gagatgccat aaccaaggca ttcgacgcat gtcagaagca gtataattcg gatactgttg
     7741 attttcttgc actcaaggta accgcggatt ttgagcaaaa aatcaagaat gacaaaatct
     7801 cggttgagag cattcaggac agcgttgagt ctgttcttat taaagcaggc tacgacgatg
     7861 ttgccaaggc ttatatcctt taccgtaagc aaagagaaaa gatcagaaat atcaacgcaa
     7921 cggttcttga ctataaggag atcgttgaca actatctcca tgttaacgac tggcgagtta
     7981 aggagaattc caccgttact tattccgtag gcggtcttat cctcagcaac tcaggcgcta
     8041 tcactgccaa ctactggctt tccgaaatct acgatgatga aatcgcagag gcccacagaa
     8101 atgcggatat ccatcttcac gacctttcca tgctcaccgg ctactgtgcc ggctggtcac
     8161 tcaagcagct tattcaggag ggtctcggcg gtgtaagagg caagatcacc tccgctccgg
     8221 ccaagcatct tgcaaccctt tgcaaccaga tggtaaactt cctcggcatc atgcagaatg
     8281 aatgggcagg cgctcaggct ttctcatcat ttgataccta tcttgcttcc tttgtaaaat
     8341 atgataacct tgactacgaa tcggtcaaga agtgcattga atcctttatc tacggcgtta
     8401 acacaccgag ccgttggggt actcaggctc ctttctcgaa catcactctt gactgggtcg
     8461 ttccgaatga ccttgcagag cttaactgca tcatcggcgg caaggaggta gacttcaagt
     8521 ataaggactg ccagaaggaa atggacatgg tcaacaaggc gttcatcgag attatgatcg
     8581 agggcgacgc caacggcaga ggcttccagt atcctatccc gacctattca atcacaagaa
     8641 actttgactg gtccgacacc gaaaacaaca gacttctctt tgaaatgaca tcaaaatacg
     8701 gtactccgta tttctcaaac tacatcaaca gcgatatgga gccgagcgac gttcgttcaa
     8761 tgtgctgccg tctgcgtctt gaccttcgtg agcttcgcaa gaagtccggc ggtttcttcg
     8821 gaagcggtga gagcacgggc tcggtaggtg ttgtaacgat caatatgccc cgtattgctt
     8881 atctcgctaa ggacgagaag gacttctatg agcgtcttga cagacttatg gatatttccg
     8941 cccgctcgct caaggtcaag agaaccgtta ttacaaagct tcttgacgaa ggcctttatc
     9001 cctacacaaa gagatatctc ggcaccttca acaaccactt ctcaacaatc ggtctcgtcg
     9061 gtatgaatga ggtcggtctc aacgctaagt ggctcggcaa ggatatgact gatcctaaaa
     9121 ctcagcagtt caccatcgac gttctcaacc acatgagaga aagactttcc gattatcagg
     9181 ttcagtacgg cgacctttac aaccttgaag caactccggc agaatccaca gcttaccgtc
     9241 ttgcaaagca cgacgttaag agatatcccg acatcaagac tgccgctcag catgagggcg
     9301 acacaccgta ctacaccaac agctcacatc ttcctgtcgg ctacacagac gatattttct
     9361 cggctcttga cattcaggac gagcttcaga cgctttacac ctcgggtacg gtattccacg
     9421 cattcctcgg tgagaagctt cccgactgga aggctgccgc aaaccttgtc agaaagatcg
     9481 cagagaacta taagcttccg tattatactc tttcaccgac atattctgta tgtaagaacc
     9541 acggttatct cacaggcgag cagtacgttt gccccgagtg cggcgagcac actgaggttt
     9601 acagccgtat caccggttat taccgtcccg ttcagaactt caacgacggt aaggctcagg
     9661 agttttcaga ccgtaaggtt tatgatattg ccaattctca tcttacccat cacggcgtaa
     9721 aaaaggaagc aaagacagac gaaagctcta aggttgaagc aggtgaaaac gccagctatc
     9781 ttttcacaac ggcaacatgc ccgaactgca agattgcagc cgcaactctt gacaaggcag
     9841 gcttcgatta cacaaagctt cttgccgatg aaaacgctga gcttgcacag gagctcggta
     9901 tcaagcaggc accgacactt gttgtaatta aagacggcga agcaacgaaa tatacgggtg
     9961 tttcggacat caaaaaattc cttaagaatt aacaggagca attatgtacg acataattat
    10021 aattggcggc ggccctgcgg ggctgaccgc cgctctgtat gcttgcaggg caaataaaaa
    10081 aacgcttgta atagaaaaag agacttttgg cggacagata accttttccc caaaggtcga
    10141 gaacattccc ggctttataa gccttaccgg taatgaattt gcggaaaagc ttgttgaaca
    10201 ggtgctcgag cagggagcag atgttgagtc ctgcgaagtg cttgaaatca aggacggcga
    10261 cattaaaaca gttgtaacag acgacagcga atatcaatgc aaggctgtaa tcattgctac
    10321 aggcgcaaaa caccgtatgc tcggcattaa agacgaagag aaatacgtcg gtgaaggaat
    10381 ttcattctgc gccgtatgcg acggagcttt ctataagaac aagaccgttg ctgttgtcgg
    10441 cggaggaaat tccgctttac aggaggcaat tttgctttcc gacctcgcaa aaaaagttta
    10501 cgttgttcag aatcttgatt ttctgacagg cgaaaagaag ctttcgaatc agctttataa
    10561 gcttgaaaat gtggaggtaa taacgggagt taccgttgaa tccttcctcg gcgattcgga
    10621 gcttaaggga attgtaataa aagattcggc aggggagagc agagagcttt ctgttgacgg
    10681 acttttcatt gccataggtc ttatcccgca gaatgaagtg tttaaaaatg taattgagct
    10741 tgacggcaga ggctacgcaa ttattgatga aacctgtgaa tcggcaacca ggggattttt
    10801 tgttgccggc gactgccgca cgaaaaaaat tcgtcaggtt acaaccgccg ccgccgacgg
    10861 agcaacagca gccctcgcag cgtgcgatta cattgattct atagaataat tgattacggt
    10921 tgcaaaagac ttctccgatt ttgagaagtc ttttttattt agcaattgac ctcatatcaa
    10981 tttttctgta taatactcac gaggtgattt tatggcaata ctgacatata aaggcaacaa
    11041 attcttcctt gacagggagg aatatactat aatttcaggc gcaatgcact atttcaggat
    11101 tccgagagaa tactggcatg atcgcctttt aaagctaaag gaatgcggct tcaacactgt
    11161 tgaaacatat acctgctgga atcttcacga accgagagaa aatgtttttg acttttccgg
    11221 tatgcttgat cttgaagcat atatcgacat tgccaaagag cttggactga atgttattct
    11281 ccgtccgggt ccgtatatct gcgccgaatg ggagatgggc ggactgcctg catggcttct
    11341 caattatccg aatttgacaa ttcgctgtaa tgatgagctg ttcatttcaa aggttgagcg
    11401 ctactataac gagcttctcg ggcgcattgt tccgaggctt tcaacaaacg gcggaaacat
    11461 cataatgatg cagattgaaa acgaatacgg cagctacggt gacgaccacg aatat
//
LOCUS       DAWBSW010000049        11448 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1221_length_11448_cov_2.944615, whole genome shotgun sequence.
ACCESSION   DAWBSW010000049 DAWBSW010000000
VERSION     DAWBSW010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_11448_cov_2.944615"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4946..4955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7292..7301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3169 a         2650 c         2190 g         3419 t
ORIGIN      
        1 atttcaaaca cgttcgtgtt taaatcctta atccgtgagc aaatccacgc ctgtttcacc
       61 gtcgatacac tcaaccttga ccgataccgt ctcatttctc gaggtcggaa tacttttgcc
      121 gatgtagtct gccttgaacg gcagctcacg gtgtcctctg tcgatgagca cggcaagctg
      181 tatctgcgac ggtctgccca ttgtaaaaat cgcttcgatt gccgctctga ccgttctgcc
      241 ggtgtatata acgtcatcaa ccagaactat ctttttgtcc gtaacctcaa atcggtcatt
      301 ttcggcgtta taaatcggag cttcgctctc cttggttaaa tcgtcacggt aaaagtgtat
      361 atccaaggtt tggaacggga cgtcaacgcc ttcaatcttc ataatattgt cgtgtatcat
      421 ctgtccgagg ataattcccc tacgcttgat tccgacaagc acaatgtttt ccgtcccctt
      481 gtttttctcg attatttcgt gtgagatcct cattacggct cttccgagag ccgcttcatc
      541 catgattttt ttattcatgt gagcctccga aaaaagaaaa tgccctgtcg cacagtgcga
      601 caagacacaa aattacataa gtatatatgc agaccttgct ccgcttcatg taaaatcttg
      661 tcggcatcac tgtaccgctc ttaaagagtt cgatttattt tataccagaa agtatgattt
      721 gtcaatcact tatttataat ttgttcacat tttacggttt atacaaaaaa acagaccttg
      781 aataaatcat tcaaggccat tttgtataag cacgggcgtt tcatcatcgg taataagtgt
      841 aacggttttt ctcgtgtcaa gtgtatcata gtatttcttg aagcgaactg caacacgtac
      901 ccgtttgccg caattcggac attcatatat cgcgcggaaa tattgattgg aatagcgcca
      961 tttctttata agcttgccct tgactccgca ttcctcgcag gtataattga gtaaactttt
     1021 atcaacaagc ggactgttga tattgctgat tgcatagggc ttgaaaaaaa gcttctcata
     1081 aaacgcattg tcacagggca aaatatatct tgcaaaatca tcgctgcgaa ctcgcctgaa
     1141 gcattcggcg gtcaacagac tgtcatccag cgccctatgg tgagaaaagc tgttgggatc
     1201 cattcccaca tcctcggcgg cattaaaaag accgagctgt ttttcaacag gtatgtttct
     1261 tttaatctga aaataacgct gaacgtcagt atagtttgac aaaaacggaa cggtctctcg
     1321 acccgtaaga aagcggaagt tttcaataat aacacgaata tcgccgttgc cccatgtgag
     1381 aataacgttt tccctgtcgc cgatccagtc ttgaaacttc ttcatcaccg cttcaaaggg
     1441 ttcgccgctt ctcatatcgt catttgtaat gtgcgtcagc tttttaatgt tgctgcgtat
     1501 tttcttaccg attcttgatt taacgaagca tgaaaactcg cttatctctt caagctcctc
     1561 gttgagcata acggcgccga cctcaatgat ctcgttcatg aagctcttgg ttcggtgaac
     1621 aaaagcgttg ttccactcga ggtccattat aatataatac atacttctcc tttcatcttc
     1681 cctcgaaata accactgatt aaagcaaagt actgtcctca gcggttatat aaataaagta
     1741 taacctataa cggtgtttat ttcaaggaaa aagagaaaat gatttcataa gtttttacaa
     1801 aactctttac aaagcacaaa aagtatgata aaataactct actttgaggg tgtagctcag
     1861 ttggttagag tacttgcttg acatgcaagg ggtcgatggt tcaaatccat tcatcctcac
     1921 cagagtaaag tcctgttctt cggaacggga cttttttgct aaccccatcc cattaagctg
     1981 agttgattta acagcttgtt tatgctataa tttttttcag aggtgatcga atgataccca
     2041 aaaagattca ctattgctgg ttcggcggtg cagataagcc gaaatccgta aaaaaatgta
     2101 tatcaagctg gaaaaagttt tgtcccgatt atgagattat tgagtggaac gaaagtaact
     2161 atgacatcaa ttcaactgga tataccaaaa tgtgcgccga agaaaagaaa tatgcttttc
     2221 tcagcgattt tgcccgtctt gaaataattg agaaaaacgg cggaatatat tttgacaccg
     2281 atgttgagct tattaaaaat attgatttcc ttcttgataa cgaggcgttt ttcggtctgg
     2341 aaaaccccga ttacgtcgca acgggactcg gcttcgggtc tgttgctcac ggggaagcca
     2401 ttacagcgat ggttaaagaa tatgagccgt accttgacgg aaagagcggc acggttacct
     2461 gcccccgact caatacggat gccctgcttc gctacggact gaagcttgac ggaaagatgc
     2521 agaccgtggc aggtgcactg attttgccga gcgactacat gaacccctac ggcagcaaca
     2581 cgggaatcat gagcaaaacg gaaaacacaa tttctattca ctggtattcg ggaagctgga
     2641 tgtcacagac gcagcgtatg aaatcaaaaa ttacaaaacc gattcacagg gttttcggaa
     2701 ttgacttttt aaagaaaaaa taaaaagaaa gcgtggccgt aacggtcacg ctttttactg
     2761 taattcagtt tacgcaatga ggtcactgac gcccatcggc ttgaagcctt caagaagcac
     2821 gccgataatt cttttgaccg aggtatcttc aaccgttacg gtcatctgcg ttgacttgcc
     2881 ctctgaattg attgcagtta ttactgccgt tccgccggaa acgccctcta caacgccgtc
     2941 actgcttaca acagcaacat gatcgtctga tgaagtccag cttgtaattt tctttccgcc
     3001 tttaacggac ggagtatagg tctccatata tgaaagagtg acatttttct cctcaaattc
     3061 ataaggaatg acctttggca cggctttctt tgcaacagtc ttttttactt ccttctttac
     3121 agcggcggca cttgaggtcg atctgtaaag cgatacgccg taagtgatat cagcgatata
     3181 ctcataggtg atataatgca cggcgcagag atacgattca acatagttgc tgtcaaaaac
     3241 ataaaatccg ttctcatcac atgaaataaa aattgacgaa tgctgcgaat aagctgttgc
     3301 ccactgaatg tagtcacccg aagccgcttt gtgcatgatg ttataaaaat ctttgtatcc
     3361 cgccgagctg agtcttgcga tctcggttgc cttgcgcgga gtcggaacat aatagctgta
     3421 agcgcccgaa ttataagcgc cgacaccacc gtttccgtaa agctcgttaa acatcatatc
     3481 ggcaaagccc ttacaggttg ttgcggttct gaacgaaccc gtccagtaaa cgccctccca
     3541 ttcattgatg agctgctgaa gctttgcatt cgtacaatta tatccttttg cgcttgcagt
     3601 gttaaaagaa gcgaccgtaa ggattgtaac tattgccaaa agaaccggca ttattctttt
     3661 tataagtgtt ttcataaaaa taactcctgt cggtagtttt tacacaacgg acaggagtat
     3721 aacacaaaag gttacaaatg tcaaatcttc agatgcttaa attgtgactt ttattccaaa
     3781 atttcaattt agatcaaaca aataagccca ttttattgta tattatgcaa tatattttat
     3841 cttttagtaa gcattatcat gttaataata aacaaacttt ataaaagtta caaaaagtta
     3901 caatttgtaa tcaatttatg ttcagatata cttaatatag ccctctcttc tcggagctgt
     3961 ttctttcggt tcaacggccg gatagccgag agcaattgat cccacacctc gaagcgtttc
     4021 gggaagtccc cattcacgga ggagtttctt gccgtcctcg ctgtcaaata tctcacgttc
     4081 acggtgaacc caaacagagc cgagaccctc ggcttcggca gcaagcataa gattttcaag
     4141 cacacagctt ccgtcctcga caaaggtgtt tgcattgccg tcggcaagca ccagaactat
     4201 cacaggcgca ccgtaatagg ggtcgctatc cgtgcgcaaa actgcggcat taagtgcgga
     4261 aatcctatct ctcgtcatct tatcctttat tgctataatc tgcggtgact gctttcctct
     4321 gcctgtcggc gcatatgttc ccgcatcaag aattcttgca agcttatcgg caggtacttc
     4381 atcactcttg tatcctctga tacttctgcg tgacttgatt atatcataaa aatctttcat
     4441 aacaaaacct ccgtttcttt ttctatatac aattataaca taaatgtcaa gaaggtattg
     4501 attttctacg ctttaaaatg gtataatatt ttcagtatca gcggatacac aaattttaaa
     4561 gatacggagg aaaaatatga tagttaattt tatgcaaaag cttattgcgc tcattctttc
     4621 agctcttatt tcagccggaa ttattgctcc tgctccgatt ccgcccgatg gcgttaaagc
     4681 aaattctaac tttgttttcg ccaatgaaga agccggttca gccgagggca cagcaatggt
     4741 aacggcgaat tttgacgcaa cttatgaact gtattggggc gatgcacagg gcaacaagct
     4801 tacaacaagc tcaccgagcg gcaagattgt tccttacagt tggtttgcac aggttgacgt
     4861 caagaaaggc aagggcgagc acgaaacaaa ctcattcctt gcgattcccg acggcgcaga
     4921 aaccattctt ctctattatc aggatnnnnn nnnnngatca gcttcttgac acagacaaga
     4981 ttcctgagga aaacattccc gattacggtg acatgaccta cagcttcggc tcactcagcg
     5041 acgttcactt cggcagatat tttgatgacg aaggcaatga ttggtccgat acctcttatc
     5101 cgcaggctct taacttcctc gacgacatgg gtgtttcaat agtcggcgtt tcaggcgatc
     5161 tttcatatga aggcgagaca tcttcatatg aatcattcca caaatacaac gatcagcacg
     5221 acttcaatgt tttttcctgc aagggcaacc acgattgcag agacaaattt gactataatt
     5281 catggaaagc aaacgtaaat gtcggcgtat tttccgagaa caaaccggca ggtgttcttg
     5341 acgtagcaga taacggatat gacttcgttt actcgggcaa agagacaaac ggcgatgtat
     5401 tcattttctt cagccaagtt aaagacgcat acgttccgtt cgttcagatt gtaaccgacg
     5461 agcagcagga ttggcttgaa gcaatgtttg aaaaatacaa ggataagaga gtttacctct
     5521 atttccatac cttcctcaat gctccgaagg gcaacccctt ccttggtgaa ggcaacattt
     5581 ataacgactg gggtcttttc tatacaattc cttatttcaa gggcaacaag gacgaaagac
     5641 gtttcagaaa gctccttgaa aaatatcata acgttgtatt cttcaacggt cactctcact
     5701 gggcatatca catggagtgc tacaaccccg accttaatat ctcggactat gacggaacaa
     5761 cggcaacaat ggttcacgtt tccagcacag gcgcaccgag aacaacgagt ttcacccacc
     5821 cgacaaagaa atcaaacccc ggcataatgt cagagggtat ttacgttcag gcatataagg
     5881 atttcatcat cacaaacgga tgtgacttcg ttaacggtca gttccttgct tatgcaattt
     5941 ataaaattga caacagataa aaatttatgc taaaaccgac gcctcggaga ttctttgtat
     6001 agaaatctcc gaggtgtttt catgtaaaaa gactgccggt aatacagcag ccttttgaat
     6061 atttatttaa ccgactatta cagtcacaac accggtgacc tctcccttta gttcaccgtc
     6121 ttgatatgct tgcgccgaat atgagcaatt atattcgcct gccttaacaa cggttgacag
     6181 cttatccttt tcaaggcact gacccggttt tatcatcgga gacgtataaa tcatttcgcc
     6241 gtcatcctta taaattatga acttgatatc atactgacag ctctcggggt tttcaagcat
     6301 cacactacca agcgaatagc ttttttcaaa cacaatgcgt ttatttatca agtagcgtat
     6361 ttcaccttcg ggaacgcttg ttatatgtgc atctgcatca acaccggtca gaattccgtt
     6421 actgacctta cctccgcccg caagtgacag ccttatgcga ggtgcactgc ctgtcaatgc
     6481 aatcacgcac agcaccacgg caaccacagc cacagcaaca aatactattg acttttttga
     6541 tttattcttc gccattgtac tccacaccga ccgcaatttt aattttagca tatcttgtgc
     6601 cgattttttt atttgtattt ttgtcatagg cgttgacagc gaccttgaca tcatgtattc
     6661 cgtaactcat ttctccaaaa agcttggcac ggtttatttc cttgcccggt tctagtccga
     6721 gagaacgata aagcacggta ttgtccttat cataaaccgt tgcaatcagc acaaaatcat
     6781 ttgttttcgg gttgccgaag cataaatcct ccgttgtaaa atcatctccg atatttacaa
     6841 ccgaattaaa ataaaagctg aacttgcgac tgccttttga ttttatcgca tcaacaaaaa
     6901 cctgctgctg ttgaacgttg aatatactgt ctcctgcaac cgtttcactt tcaactcttc
     6961 cgttgaactc ggctgccctt atatcagcag ggacatttac aagaaccgat cgagaaatgc
     7021 aaatgcttgc tgttataatc aaagatatcg gcaatataat gcaggtgaaa atgcgaaaat
     7081 aattcaacga tgaaagaaac tttttgcttg tgtaatagcg gattttctta acaacgcgcg
     7141 ccttgcgacg tttatgtaat agcttagctt tttctttctt tgcttttctt tcagccgtcc
     7201 ttgtctcctt tgcggaaatc ttttggcgct gtctgcgaat ctcctcactg cgtttttcgc
     7261 gctgcttctg tttctttctc ttctcctcaa tnnnnnnnnn ncctcaatca gacgctttgc
     7321 gttcttctcc gcttcagctt ttttacgctg tgcatccttt ttcttattat aattgttttt
     7381 gcctgcgaca gttttacctg ccacaggcac atttttctta tttgacatag aaatcaactt
     7441 ccattattcc cacggtaatt tttcaatatt ccaaagctgc ggataacggt ttgtttgtac
     7501 tgcgtcaacc tctatcaggg ctttaactgt tgttcctgcc tcaacatcag agacatcaaa
     7561 cttgctttca tcaaagtcac tgatgagata gagcgtgtca ctttttgctt ttcccgagcg
     7621 atcgccgccc ataaaatata cacagtaatc tgtactgcgg ttgtcatacc attcttcgtt
     7681 aaaaacatac ggaacgtttg ttgtgaattt tgtgatcgaa ccgtcggcaa gctgccactc
     7741 aaaagcagtc ttaactctga ggcaacaggc tgctccgctg cgatgaacaa tcaccttgag
     7801 ctttccgata taattctcat cgtccttatt aaggctgagc gtataaagcc cattttcgtc
     7861 cttgaatttt tcagcatcaa catctctttt attattagca aaataaacag ttgccgaagc
     7921 tttaatgccg gaaagaatga agctttggga ctttgaggtc tgagtaacga accacgccaa
     7981 agaactgcct acaacggcaa ttattgcgat aaccgcaacg ataacaacta atgctttttt
     8041 gcttatcttt tttgacatat tcgtcacctc gatttcctta gccgattacg ccgtctttat
     8101 cctgcgttgc tgtcatataa attcttgttg caaaggtgag cgattcaccc tgcgcaagag
     8161 ctgtgctgac ctcatcatac tccacccagc ttgcgatctt caaaactttc gattcacccg
     8221 gagccaaaag cacatatttt gaactgttgt acatgttcag ctccgtctca gtgagagagg
     8281 gcaggtttgc tttgattgtc tcctttacac ttccgttaac aagcatgtca tcggtatata
     8341 caaaccagcg ataacctttt gcaccgtttg taacatcaac attgatgtat actcttgccg
     8401 gaattgttcc cgaattggtg acattgatat catcaaaatt gaccgcccga tcaaaaagct
     8461 tttcattttt gtctgcaagc ttggtcgcag catcaaaaat tacactgtgg tttataacga
     8521 attttgtgtt aacggagaga tttccgaaaa caaagcgatc gccgctgtca atgacgccgc
     8581 tatcataaaa ccacgcggag gtttttgaca ttcccgaaaa atatcctatg caaagaacaa
     8641 gaacaagagc cattatcggc gtcataattc ttttaacctt attcatcgct gtcacctccg
     8701 ttttttttct tcatatgagt taaaagcata tttaatacaa caagcgcaac tgctgcaatc
     8761 gcaagaatta tcaaaccttt tatctgagaa acaccgccaa atgctattga aaaatatccg
     8821 agcagcggca cgctgtacca catcttgccc acaattctat cggcaggagt cggcatcggg
     8881 tcatcttcgg gattgttgtc gccccgtgtt gttacagttt gattatctct gtctatagca
     8941 acaattctgt gggtaaagta ttcatcaagc tcctttgagc cgaccgtaac gacatcgccg
     9001 acatcaagct ctgcgaactc ggcttttttt gaaaagacaa gatcgcctcg atgcaaggtc
     9061 tccgccatgc tgtcactggt cacggcatat ccttttgcac ccgaaagaag attaaaagca
     9121 acaaaaccga ccgaaattac ggtaattatc gcaagaatat taaataaaac attgccgacc
     9181 ttcggcttca ctctgtcatt cttcatgcag ccgtgccggt gagctgataa gaatctatgc
     9241 tgttccacga aacattgtca gcctgcttag cctcaaaaat tacctttaaa tttaccggct
     9301 gtcctgcata ggcctttgaa atgttttcct tataggtaat gcttgaaatt gcgtcaatat
     9361 aagagatctc agagctgatt gacggaacag cgttcaagtc ggctatgctc tctgactcat
     9421 acttgtcgcc catatagtag aaataacaag taccgcttgc gtttgtgttc tttgaccact
     9481 tgtttgcggc aaaggtaaca tcgatatcat ctccgctgcc tgaataaatc ttctgctccg
     9541 cttttcccga agcatagctt gtatattcga tagaaagtct tatctgaaca tcggttgtcg
     9601 agccgttatt tattctgagc attgcaccct gtccgtcgag gtttacaaga ttgtctccgg
     9661 gcataataat ggttttctca tcactgtcta agtcagtaag ctttgcggtc tgcgccgcgc
     9721 tgacaacgga aaccgaaatg ctctgcttct tttccagcgt tgtaacgaac catgcatagg
     9781 aggttccgca gttcataaga ccaatcacaa ccgccaacga cgctacaaaa gcaattattc
     9841 ttttagtcat aagctttctt cctttccgct ataaacagct caatctcaat cgccgcccat
     9901 gcgatgtaaa ttattgcaat cacaatctta acggcagtat atcgcaggaa cttaacgaga
     9961 taacccagat acgggatcgt tgcttcgacc ttgccttcgg catactcggc cgatgttttt
    10021 ccaagatcgt tttcctcgtt tgcatcgccc tttgtcacaa agctttgcgt ttgttcatca
    10081 atttcaacaa ttctgtgagt aaagcttttc tgctgataat tatcggtaaa ggtgacaata
    10141 tcgccaactc tatagtcctc aaaattatca taaggcttta tatatatcag gctgtacggt
    10201 tttattgtcg gagacatgct gcccgtttca acaaccttca tatccatttt gagaattttc
    10261 ggagtataaa caactcctac agccacgacc gaaatgcaaa taattattgc ggtcagaagc
    10321 atattaatct tttgttttcg aagatttttc atcttcttca tccttctgat ccgagaattt
    10381 atattcggca atcagctttg ccaaattaac cgcttcgccc aaggtgataa aaatcaaggg
    10441 caaaacaata aaaataaata ttattttagg atttctcagc acatccaatg ccgagcttcc
    10501 gaccgttcct acattaagaa ttaccttgcc gatcagatcc acttcaaaaa cagccgccgt
    10561 atcgacagaa tcatttgcgt cacccttggt gagaaagatt ttttcgccgc tgtcgctgtc
    10621 ttttatttca actattctgt gggtgttgat ttgaccgctt attgtcatat cgtttgaata
    10681 aaaagaaatc acatcgccga ctttgagcga ggaaggtttt gttttgtgaa caataacaat
    10741 ccctccggcg gggatttccg gctccatgct tcctgtctgc acttgaaaca cactgaatcc
    10801 cagcacactc ggcgctttac cggcggaagc gctgaggaca gatacaaaca ctgtaaatcc
    10861 gagaataaat ataagtgccg aaagcaccgc cattattctt gagaaaattt ttcctgcttt
    10921 tttcataact tatgaaaccg gctgaacgtt gattttaaga gtgccgagcg acgatgacaa
    10981 accttcgatt gaggacggac tgtctgccgc aagacctatc tcataataaa taataaacac
    11041 atcgttcttc tcggtttgac tgtcggcaag cgatcttccg tcaaaaatct ttccgtttga
    11101 aagagacgaa agcctcatgt aatccgaacc gaaaagctgt gctccgtcgg taaatgttac
    11161 aactccgttt ttcacagttt ttatcgcctt atacacggta tatttaacct gaacggaatt
    11221 ttttagatcc gaattcatat ttgacgggag agaatcgatc gacatgctta acttaagctt
    11281 ttcatcggtt gcagttgtaa gaatcatctt atattttttg taggtgcccg atgtccaatt
    11341 gttaagctca atatttcctg ttaatcttga ataatttccg ctgttgtcgg cagtatagta
    11401 tgctacgctt gcatacgtcg gactgacaac ctcccccgta attgatgg
//
LOCUS       DAWBSW010000050        11358 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1234_length_11358_cov_2.749182, whole genome shotgun sequence.
ACCESSION   DAWBSW010000050 DAWBSW010000000
VERSION     DAWBSW010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_11358_cov_2.749182"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2862..2871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2702 a         2896 c         2296 g         3454 t
ORIGIN      
        1 gcctcggagc acttcatatt aagctcttca attttctttt ctgctttttc ggacctttct
       61 ttcagctcgt ttttttcttc ggtaagagtt tttacgagcc tatccttatc cgaaataata
      121 tttttatatt cctgctgagt ttttttcaac tgctgaatat agtttattac ggactgacgg
      181 tcaaatccgc cgaagggagt ttttttgaaa agaacctctt ctttcattct gtaatcattc
      241 ctttcacgat tacgtattta accgatatac attatcttcg ttacaccgtt gccgcacttc
      301 acggctccgt ttttcagcga atagcagtac atttcgccgc ttccgctctt ataattcttg
      361 atatatgcaa acgcgctgtc gctgtaaacg aaattattca gattgaggtc gctgtcaaca
      421 acggcatagc ctgttccgtc gtatattcct gccgttacaa gttcctcgga gttgctctgt
      481 ctcgtcaaaa ctatcgtcag tgacttattg acggcgaaat aagaatatat attcttgcaa
      541 atctccgcct tgccgccctt tggttggaat ttataaagtg ttcgtgagcc cgaggattca
      601 agcttctcat agaataatgt atcttcgatc accgtgcagt cggaaacctt tgaatctatc
      661 agtcttctgc tgctcagctc ggcgttttcg gcactgctgt aatacaattc tccgtcaaca
      721 aggccgtata ttgtgcccat atcggcaaga ataaaccgag ttctgtccag atcatagcct
      781 ttaagctcat attcaaccac agtcgaaccg tcataccatg agcaaaagct gctgtcgctg
      841 acttcatacg agctttggac agcattgcgt acataatcca tcgccttttg cacacttgaa
      901 ttttgagccg tttcaacaag cgaatccatg ctgattatat ttccgactcc gataaccgat
      961 tttctgtaaa tcagcctcgg atctcctgcc ttggcaaatt caataatgtt ctcaagagtt
     1021 atgcctgaaa ccagcctgtg cgaagcaccg ttgctgtaag cgaagcaggt gtactcatcg
     1081 ccgactgcaa gacccaaatc aatcttgtcc agctcctcac gaagctgatc tctgattgcc
     1141 ttgagcttat aacggtcgag tgcggcattg tattttgccg tgtcaagaac ctttctctta
     1201 aatataaagc ccttttcaac catgtaatcg ctttcaaccg gcttttgaat ttttttatcg
     1261 ctttcgtaat agttgtccga tataaaatcc tgccaattga catttgactt atttcttgtg
     1321 aaataataaa gattgcctcc gtaaacgaag tcattgagat agacctcgct gacgtccgcg
     1381 catatttccg tcggcttttc gtcgcccttt actgaatata tattcacatc tgtacttttg
     1441 ccggtcggct gagtataaag tatctcatag tcattacggt caccggcata cttgacatgg
     1501 ctgatgccgc tcacaacatt tttactgttt tcgccgaatt tcgccctatg aagcgaatag
     1561 acgttactgc ttcgacgtgt gaaataaact atattgccaa ccgacggcgg aaaatattct
     1621 tcaaccgact cggcaaccgt ctctgccctt tgcatctcgg tgctgtacat atagcagctt
     1681 gttatatttg attttgtgat tgcatagcag gcaaaaagac cgtccttggt cgttttccag
     1741 ccggcatcaa tgcccttctc aataaatttg ccctgctttt tcaaggattt tttgctgccg
     1801 acctcaatca ttctgaggtc atatttaccc gttgcggacg ctgtattacg gctaaaccat
     1861 atatacattc cgtccgtcga aacggaaatt tctctcgcct ctccgatatc gtaatcggtt
     1921 ccgtcggcaa gcttaattgt tgtgcttttg tctgttgtgt tataaatcgc agtcaccgga
     1981 aagagatttc tgtcagctct ttcggctccg aaatataccc ctgccattgc gctcgcaaca
     2041 ataagtatca gcgcaaggca agcaacaaac gtttttattc ttgaatccct ctttgaggga
     2101 atttttttct tctttattga taatttttta tccgaatttc tgttttccga tttctttgtt
     2161 ccgaaatcag cggcaatttt ttcaggctcg gaaatatcgt caagcggctg aatatcgccc
     2221 gagaatccca ttgcgtcata aattggcaaa agctcagact cctgaactgc ctgagcgtca
     2281 ctgtcatatt cgtcttttga aagtctgatg tcgtcgcttt ctttcatctt tttctccgag
     2341 tcccgttccg cccgaaaaca agcggaacgg gatgataaaa aataattatt tcatcgaaat
     2401 ggctttcttt gctttttcaa caatatttga agcgcaaagg ccgtatatct tgagcatctc
     2461 aactgccgga ccggagccgc caaacttatc ttcaacgccg actctaacaa ccggaacagg
     2521 cttgccctcg cagacaacct ctgtaaccgc agagccgaga ccgccgataa tgctgtgctc
     2581 ctctgccgta acgattgcgc ccgtctcttc agccgccttg agaatgatgt ccttatcaat
     2641 cggcttgatg gtgtggatat tgataactct tgccgagatg ccctcttcct tgagcatatc
     2701 cttggcaatc atagcctcat taaccataag acctgttgca acgatagtaa catcgctgcc
     2761 ctctgcaagc tcgatgccct ttccgatctc gaatttatag gtatcgggat cattgaagcg
     2821 cggaactgca agtctgccga aacgaagata aaccggtccc tnnnnnnnnn ntgccgctgc
     2881 aaaaacagcc gcacgagctt caacatcgtc cgccggattg ataatcgtca tgcccgggat
     2941 agttctcata agagcgatat cctcacagca ctgatggctt gcgccgtcct cacctacgga
     3001 aatgccggca tgagttgcgc cgagcttaac gttgaggtgc ggataaccga tagtatttct
     3061 gacctgctca aaagcacggc ctgcaaggaa cattgcgaag gagcttgcga aaacggtgtg
     3121 acccgtcgtt gcaagacctg cggcaacgcc taccatatta cattcggcga ttcctgcatc
     3181 aaaaaatcta tccgggaatg cctttttaaa catgcctgtt tttgttgcgg cggcaagatc
     3241 ggcgtccata acaataaggc tctcgtcctt ctcgccgagc tcaaccaaag ctttaccgta
     3301 agcttctctt gttgctgttt tgattacgtc tgccatcttt tacgcctcca attctgcaag
     3361 ttgtgcttta agctcgttca tagcctgctc atattgctcg gcattcggag ccgttccgtg
     3421 ccatgagcac tgatcctcca tgaaggaaac gcccttgccc ttgaccgtct ttgcaataat
     3481 tgctgtgggc ttgcccttga actcggctgc tgccttgaaa gctgcgtcga tctcgtcaaa
     3541 tgaatgaccg tagatttcaa ttacattcca gccgaatgca cggaattttt cgggaatcgg
     3601 atacggagag ttaacctctg cgatggagcc gtcgatctgg agattgttgt tatcaacaac
     3661 ggcgcagaga ttgtcaagac ccttggctgc ggcaaacata gctgcttccc aaacctgtcc
     3721 ttcttcaatc tcaccgtcac cgagaactgt gtaaactctg taatcctttt tgtcaatttt
     3781 tcctgcaaga gccataccga ccgccgttga aataccctgt ccgagggagc ctgtgctcat
     3841 gtcaacgccc ggagtacgat tcatgctcgg atggccctgg agcattgcgc ccggcttacg
     3901 aagattttta atttccgata cggggaagaa gcccttgttc gcgagaaccg cgtagagagc
     3961 cggagccgta tgaccctttg aaagaacaaa tctgtctctg ttctcatcct tgggattttt
     4021 cggatcaaca ttcatgtggt cgaaataaag atatgtgaga atatccgcta ctgagagcga
     4081 tccgcccggg tgacccgact ttgcattgaa cacaccttcg atcacgccca tacgaaccgt
     4141 acaagccttg atctgtaatt gttttttagt tactgcgtcc aaaaaaacac ctccaattat
     4201 tcgcttaaat tttgtttttt caagaaatcc ttaaaataat ccggaacctc tgcactgaat
     4261 tcaaggtatc ttccgtctct cgggtgtttg aacccgatca agcctgcgtg cagacattgt
     4321 ccgttcaatg aggttactcc gttcttcggt ccgtaaatat tatcgcctgc aacggggtgg
     4381 ccgatatatg ccatatgaac tcttatctga tgagttcttc ccgtctcaag agtaacctta
     4441 atatatgcaa atttctcatt ctgatcgatc acttcataat gagaaacggc atccttggaa
     4501 ttttgataga taacgcacat tttctttctg tcattgggat ttctgccgat cggtgcattg
     4561 accgttccgc tcatctcctt gaaatgtccg tgaactaccg ctctgtattg ccttgtaaag
     4621 ctgtgctcct tgatctgagc ggcaagcttt tcatgagcaa aatcgttttt tgcaactatc
     4681 agcagaccgc ttgtgttttt gtcgatacgg tgcacgattc cgggacggat aactccgttg
     4741 attcccgaaa gactgcttcc gcaatgataa agcaaagcgt tgacaagcgt tctgtcatag
     4801 ttgcctgcgg cagggtgaac caccattccc ctgggcttgt tgacaaccaa caaatcatca
     4861 tcctcataga taatatcaag aggaatattc tcgggctttg cctcaatcgt tttaagctcg
     4921 ggcattgaga agcttatctc gtcgcctatt gacactctga acgattttga aacgggcttg
     4981 ccgttgacct tgacctcgcc gtcgtcgata atatgctgaa tatgcgaacg ggtaaagtcg
     5041 gaacaaagat ccgacagggt tttatcaatt ctctcccccg aaaaatcaga atcaatgcgg
     5101 aagaaaaaat cactcatgct tctcacccgt ctttttgcgt tccctgaaca tatcgacaat
     5161 aacgtaaatc ataatcgcaa ttgcaccgac ggttacaaga atatccgcaa aattgaaaac
     5221 tgcgaaatcc gtaaattgaa cctcaataaa atcaacaaca tatcccttta aaacacggtc
     5281 aatcagattg ccgagtcctc ccgaaataac catcacggcg caagtaagat agaattttga
     5341 ttttattttc ttcgttacga taaggactat accggcaaga attacaatgc ccgtcacgat
     5401 tgaaagcagg gtcgtgctct gcgaaaacga gccaaacgcc gctcccgtgt tgcgaacata
     5461 attccaaccg atgaatccgt taataaattc caccgagcct atcggctgca aataacgttc
     5521 aacaaagaac tttatcacct gatcgaatgc ggtcagaacc gctattgaaa taaaactaat
     5581 cagtacgctc attatttttt gaagaagccc ttgattttgc ctgccaatcc gccgtcgttg
     5641 ccgtcatcgt caaggtcgtc atcgtcaaga tcgtcgagat atccgttatc gctatcgtcg
     5701 gcggcatagg agtcaatatt ctcaaattta agcttaaagc cttcatttga gtccttcttg
     5761 ggctttgtaa acattatatc cttggcttcg gatttttcct ccgagccgta atcgtcaaca
     5821 attttttcaa gctcctcatc gcttgcatcg tcaatatcgg gaacaacaaa ctcggaggaa
     5881 tccgttactt cctctgccgg ctcgtcctca tcctcgtctg cgggatcgga ctctgcgtct
     5941 gtgtcctcgg tctcataaat catctgctga agatgctcaa cgtccatcga ctcggcttct
     6001 cttacctcgg gagctttagc ctcctcgacg gtctcctcaa aagggactgc agcgtcttct
     6061 gccggagctt cctctgtctt tacttcctcg gaagtttcct ctgctgcggg ttcttctgcc
     6121 gcttcaggct cggagctttc ctccttcgct tcttcggcaa caggctcctc tgccggaact
     6181 tcctcagtct ttgcttcctc ctcggctgtc atcttttcga taactgtctc gggcagttta
     6241 ttgatgagct caagctgagc ggcatactca ccgagaatct gctgcttgaa ctttgtaacc
     6301 tcctgcttca tggtctcaag agcggcaagc tccttgatca tgtcgcttct cgcattaacg
     6361 gatgcttcct tagactcctg aacagccttt tgagcgattt cctcggcctg ctttcgagcc
     6421 tcggcaacaa ttgcatcagc ctgatacttt gcatttgaaa gcatttcctt tgcggcggca
     6481 tcaagtctct cgttttcggt atcggttctc tccttgacct cggcaacgag cttgtctgcc
     6541 ttttcctgtg cctcacgggt aatcttctcg gcagctctct gagccgtaag aagcgcatta
     6601 cggatattat cctcgtcgtt tctgtattcc tcaagtctct gtgcaaggag atttatcttt
     6661 ttaaacatct ctccgttttc gcggaacatc tgctcgtagg actcggcaac ttctctgatg
     6721 aaggtgtcta cgctctctgc tttataagca tttcttccgg aggcttcaaa atgatgattt
     6781 gtgatttttg ccggtgttaa catggtttat tcctcctgta ttttaaacaa tatctttttt
     6841 gcacggaatt tttaatcaat aatttgaatg ttcaacctcg tcgagaagct cacctgtgag
     6901 cggaacatta taaggcgtaa ttatatatgc cctgcccgca acacgcttga tgtcaccgtt
     6961 atttgcatag gcaacaccgc tgagaaagtc gattattctt atcgttgcgt cattcgggca
     7021 agtctcaagg ttgagaataa caattctctt ttcattgagc acatctgcga ccgagcctac
     7081 gtcctcatat ctgtcaagct ttttgaaaac gacgtgagcc tttgaagaag ctgcagcagc
     7141 agccggaaca gaagaacgga tatcaacaac cttgttgtct ctttcattct tatgaccaaa
     7201 agagaaattg cttctcacgt catgtgagta atcgtccttc tcgggctctt cctcatcgat
     7261 atcaacatcg tcaaggaatt cttcctcaga catattaaac atgttcttaa atttgtccat
     7321 cattcccatt ataataaatc ctcctgaatc aatatactcg tttgccgaaa agcgacgatc
     7381 ctactcttac catagtcgag ccttccagta tcgcttcaac atagtctccg gacataccca
     7441 tcgacaagat gtccatactg atattatcta atttttttgc ctttatgtca agaaacattc
     7501 ggttcatatt cataaaaaat ttacgaattt cggcgctgct gtcgcaaatc ggcggtattg
     7561 ccatgagtcc cttgactctg acggagccga tttccgatat ttcgcaaagg gtttcggtca
     7621 gttccgaggg atcaaggccg aacttgctcg cctcctcgcc gatgtttatt tcaaccaaaa
     7681 catcggtcac aattcccttt ttagccgaga ttttgcctat ctccttggca accttaacac
     7741 tgtcaacgct gtcgatcata tcgacctctc cgacaatctg cttaaccttg ttggtctgca
     7801 aatgtccgat aaggtgtttt tcaacgccgt cgagatgcag ctcgtcacgc ttgccgagat
     7861 attcctgcac cctgttttcg ccgataaggt caatgccgag cgaaagtgca tgattgatat
     7921 aaaggctgtc aaccgttttg gtgacgccca tcaggcgcac cgagccctcg tctctgccgc
     7981 tttcaagcgt agccttttgt atgttttctt tgatacgctt atagttttct tcaatatcct
     8041 tgaagcgttc atcaactaat gctttttcca tcactgagat ttcttccttt cacgataact
     8101 tcatcgtagc gtttgatcgg gcggtagccg ctcgtcgctt tgctttcttc gcttacgata
     8161 acataatcgc ctgcatcata gattatatca ataactctgg cgttcataat atcgcctcgg
     8221 agaatataaa caacgtccac ttccttgcct ttttcatcct tttcacgcct tatggctgag
     8281 ctgttgattt tgtagcctgt gtattccttg aaaacaagct caatatcctc ggttctcata
     8341 tttgcatagg tttcgttcat catattgcac gaaaaagcca cggcaacctt atcattctga
     8401 gtcgttgata tcttttcaac aacgacctcg acgttcttaa agccgtaata cggcatattt
     8461 atcttcattt tttcgccttc ggacatgagg agtgagtatt tgctgtccat gaccgtcaca
     8521 aggtaccact tgtagcttcc gacgattttt cccatctgac ccgaaacatc gcccggcttc
     8581 ttttcaaaga gcttttcggc ttcggaaaca gtcaaatcgg agattttatt gtaatccatg
     8641 gcattctcat agccgtcaag gttacttata taataaccgg aaagcggtgc aagaacgtcc
     8701 gaagtgttaa tgctttttga ttcaagagtc tgaatttttg aattcagctc gttgaactta
     8761 gccgtcaggt caattgttcc gtctttaagt atctgcttgc tcgcaagctt attttcaagc
     8821 tcctcaatat tcctgctgag ctcggaaaaa tcaccgctgt ttgacgtcct tacaagctcg
     8881 gtaaagcttg catcaatggt cttgttgagc ttttccatat ccagagcgtt aagctcggtc
     8941 tgttcggaga tttcctggta tcttgcaaga ctttctttcg cttcaagcaa agctgaaaca
     9001 tttgccgcat cctcgtccga agcgcaaact ctcgcaacgg cgtcgccgct ggcaacacgc
     9061 ataccgtcct ttatgagagg aaccaccgtt cccgttgttt tctgatttat atattcttca
     9121 tcgcgcacca gaaaggcttt caattcaagc ttttcctgtt caacgacctc atccgccgtt
     9181 tccgtgtcgt agcgatcgcg attgtttctc acagcatcat gaaaaagata aacaaaagtc
     9241 gcaagcacaa gaaccgtgca tatcatgttt atccaagtgc ggtttttcat cctgcctttt
     9301 ctcctttcat aaagctttcg acaagcgtaa atgcgtcctt taacatatct tccctttttt
     9361 gatatttgtc aatatagagc caatttattc gttcattgcg cctgaaccat gtcaactgcc
     9421 gctttgcata tctgcgtgta gctcttttca ggttctcaac cgcttcattg agtgaaacgt
     9481 caccgtcaat atacggtcga agctccttat agcctatcgc ctgtgccgag gtcgaactgc
     9541 ttgccgtggc aagatactcc ctcgcttcgc tttccaagcc tcgttcaagc atgatatcaa
     9601 cacggcggtc gattctgtca taaaggaact gtctgtcctc tgcgtcaaga ccgatgaagc
     9661 atacgtcata cggcgaagcc tgctttgata tttcgatttg cccggtcatt gttgtgcccg
     9721 tcgttttgta tacctcaagt gcacggacaa ttctttttat attattcggg tgaagctttt
     9781 cggcatactc gggatcaaag gccatgacct cctcaagcag cttttccgcg ccctcgtcct
     9841 ctgctctttt cagaagcgaa cggcgaagct ccccatcata ggattcctcg ctgagcttaa
     9901 cattgttgag aagcgtatca acgtaaagtc ctgttccgcc gcagacaacg ggaagcaagc
     9961 cccttgaatg aatatcggca atgcattccg ccgccgcccg ctgatacagt gcaacactgt
    10021 agtcctcggt attatcaaga aaatccatca tatgatgcgg aacgttcttt ttttctttct
    10081 cggtcggctt ggcagttgca atatccatgc ccttataaat ctgcatggag tccgccgaaa
    10141 cgacctcacc gccgaagtga cggcatattt caacagccaa atcggatttt cccgacgccg
    10201 tggggcctat aacggccaca acaggtattc tcttcatccg atcagctccc aaagattgat
    10261 ttttatatgt tggatctaaa atgtaagata tctaatatct aatatctaat gtctatatcg
    10321 gtactgcccc gagcgaaaaa tgcgttgtgg gcaaatttac acaatttaga cgcggccgaa
    10381 gtttttttcg atctcatacc ttgacatttc tatcaaaaca ggtctgccgt gcgggcaata
    10441 tctgatatcg ggattgttga gaagactttc aacaaacttg ttcagctcgt agtctgatgt
    10501 tcggtccccg gctttaatag cggcacggca agcggttgag tgatatatcc aatcgagttt
    10561 ctcgggaatc aattcctttt gactggtgac aagatatcct gcaagctcca tgatgacctc
    10621 ggcaaggtct gcgtcggcga ctgctgtcgg acactcgttg acgataacca ttccgccgcc
    10681 gaaatcttcg acgtaatagc ctgctttttc gagcaaatca agattgtcaa gaaccgccga
    10741 atattcctct ttgctgagag tcacggtcac gggagcaaga agcatctgcg tttcaatacc
    10801 gtcgcggttc gccttgaatt tttcaaagag catacgctca tgcgcagcgt gcttatcgat
    10861 tatcaggagc ttttttccct gctcaacaag tatgtaggtt ttgaacgcct cgccgataac
    10921 cctataaggt tcaacctcgt gctgaacctt ttcaacaatc ggctgaacgg gttcaggctc
    10981 gattgagggc tttatttcaa ccggctcggg tttcgtttgt gcaacgggat ccggctcctg
    11041 caccgtcggc ttaaagcacg gaacgaggtc gggcatttcg tttttttcta tttcaaattt
    11101 tgagctgttg tcgttaaaaa ctatagtctc ggtttttggt gattcaaccg ttttcttgaa
    11161 ttcggtcgag gttgtgttcg tccaaaaatc ctgtttcggc tgagcttctt ttttgatttg
    11221 ctcaagctgc tgttcatata tttttatctg cttgccctcg gcaggtttcg gcataaactg
    11281 acggttgacc ttctgctgaa atgttgcctg tactctcgtg tcgccctgct gcaacgccga
    11341 ttttgcacag taataaac
//
LOCUS       DAWBSW010000051        11190 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1254_length_11190_cov_2.736237, whole genome shotgun sequence.
ACCESSION   DAWBSW010000051 DAWBSW010000000
VERSION     DAWBSW010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_11190_cov_2.736237"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5502..5511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5888..5897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3247 a         2304 c         2781 g         2838 t
ORIGIN      
        1 gaagacaagg atcatgttac cgttaagctt gttttggcgg aaggtaaaac gctttgcttt
       61 gacgctgttg ttaaaacatc cgaagcatta aagcgttggt cgattaaata ttcgagcgag
      121 ctcaacgctt ttgttgccga gaataccgaa ggtgctttgg cttcaaataa agaaacaggt
      181 aagctttcat ttgcaaattc ggttgagctt tcggctcctt tcgatattgc ggaatttaca
      241 tatttaaagg aagataagcg cggtgttgtg ctttccgatt ttacgttaag cgttgattcg
      301 tgctattata aaaacggcga gggacaggat attcagcttg gcacgaccgt tatcaatacg
      361 attcctgcaa ctcatactca tgttgagtcg ggcgtttggg tcgaaacaga gcatgcaaca
      421 tgccaacaca gcggtcaaag ggtaatgtac tgttccgaat gcaatgaaat ctgtacagtg
      481 gagtacacca cacagacgga tcatacaaca aaaaccgtaa gggttgagcc tacttgtgaa
      541 aaagacggct atatttcaga ggtctgtacc tcttgcggtc aggagataag cagaacggtt
      601 ttgccgaaaa caggtcacaa aaataccgtt gttcagcata aagacgcgac ctgcaccgag
      661 gacggatatg accgcgttgt atgtaatgac tgtaacaaaa taatcagtga aaccgtaatc
      721 aagtctccag gacataagac ggttgttgat gaaaagcctg caacctgtac agaggacggt
      781 tactatatag aaaaatgtac cgtttgcaat gaggttatca taaatgacgt tttaaaggct
      841 ccgggacaca agatcgttgt cgacgaaaag cctgcaacat gtaccgagaa cggttactat
      901 aaggagacct gctccgtatg taaagaggtt cttgtcgata agatccttcc ggcaacaaat
      961 catgcaaaca cagaagtaca gcacaaggac gcaacctgca cagaggacgg atacgaccgt
     1021 attttctgta aggactgtaa tcaaattatc agtgagactg tattcaaggc acagggtcac
     1081 aagactgttg cagaagaaaa agatgcaaca tgtacagagg acggatacag cagagaggta
     1141 tgctccgttt gtaaggagat attaaaaaac gaaataaagc cggctacggg acatcagcat
     1201 aaaaagaccg atactcttgc ggctacctgc accgaggacg gatacatcag ggaaacctgt
     1261 ctcgactgcg gaaacgtagt taaagaaaca attctcaagg caaacggaca taagtttgtc
     1321 aatgacatta aaaaagcgac ctgcaccgag gacggctatg ttcgtcagca gtgtacggtg
     1381 tgcaagacct acagcggtat cagcagagtg cttaaggcta cgggtcatgc atggggcaag
     1441 tggagagttg tcaaggagcc gacataccgt tctgtaggct tacgcagatc tacctgcaca
     1501 aactgcggag aacacatgga cgaagaaatc ccgatgatta ttgttcccgt taaaaagata
     1561 gttatttccc ccgaagagga tttcaaaatt tattgtaaga agagcgacag gctccaggca
     1621 aatgtgtatc cggaagaggc tgcatattcg gcggagatcg tttggacatc ttcaaatccg
     1681 aatgttcttg atgtaagtga ggacggtaca attaccgcaa agctcagagg aacggcaacg
     1741 gttaccgcct caacggcaga cggctccgtt accgcaacaa gaaaagttac ggttgaatat
     1801 accttccttc agtggataat catttatatt cttttcggtt ggatttggta tctttaataa
     1861 ctgaataact tatttaagaa gggggcagtg aggattgttc actgctccct ttttaacagt
     1921 aaaaattaag ggatatatta tgaaaaagat tttatcaatt attctttctg cggttatgac
     1981 tttttcggtt gtcggcattg ctgcaaacgc aaaggaaaac gacgatctca gctttgccgt
     2041 tgcttccgat attcattaca gtgcacctga ggcggagctt gaaaagacaa acgattccga
     2101 gctttactgg cacgctataa gacgctgcga aatggaaaac gaaacaggac ttatcattga
     2161 tgagttcctt aatcagtgtg ccgaaagcga cgatgtggaa tatgttctca tttccggtga
     2221 ccttgcaaac agaggtcgct cacggcctga agatcatctt gctgttgcac agaagctccg
     2281 tgattttgaa aacaaatcgg gcaaggaggt ctatgtgatc aacggcaatc atgatgcaag
     2341 caacgatcag gctactacgt ttgaacgttt taaggagatt tatgctgaat tcggctatga
     2401 ccatgccttg acaacgagag acgacgattg ttcatatacg gcaaacctcg gcaaaaaata
     2461 ccgccttatt gcacttgata caaatcatgc gactaagtca accgaggacg gtatgaccgc
     2521 agaaaaactt aaatgggtaa aagagcaggc aaagcttgca aaaaaagacg gcaggtatcc
     2581 tatagttatg atgcaccata atttgcttga ccatcttccg attcagcgtg tactcagcag
     2641 aaactttatt gttaagttcc atttcacgac cgccgaactt tttgctgact ggggcattaa
     2701 gacggtgttt acgggtcacg agcattgctc ggacgcaacg gtttacacaa gcgctttggg
     2761 caacaggctt tatgattttg caacaacctc attggctatg tatccgctcg aatacagggt
     2821 cataaagtat aacgataatg aaatcaagta tgagacgagg tcggtcaata agattgatta
     2881 tgacgctctt accgcaacca ccaagggata tacggatgag cagatatccg caatgaaaaa
     2941 ggatctcaga gccttttcaa agggttatct taaggcggga gttcagtaca gactggagct
     3001 ttcactctct ccggagaaaa tgggtattga cgaaaatgca gcttattcaa aaccgattat
     3061 ttatgctgtt gataagctga tagaattact gagaatgccc ctctgcggcg aaaacagctt
     3121 gcaatcaatt gcaaaggaat acggcattga aattcccgat tccgactacg aaacaggctg
     3181 ggatttagca accgatttgg ttgcatggca ctattccggc ggtgaacact tcgatatgag
     3241 cagcgttgag gtcacaactc ttctcagagc ctttgccgca attctcagaa gtgattttgc
     3301 cggcgttgcc gacgacgtcc ttctcaaggc ggtcaataac attctcggtg atatgggtta
     3361 cggtccgata gccgacagcc ttattaaatt ttgtatttca aaattcggag tttatacaaa
     3421 ggctgagatt tttcttgttg gtatcgcttc gcctgtactt tacaagtttg catacgacaa
     3481 tgacggagtt gatgacaata acggcactat tgagggctac ggcacggtaa atcttaagag
     3541 caatgttaca aatatcttta aaaatctcgg aagtctttcc gagaagatgg tattctatat
     3601 tcagcttgtt ttgtcatatc ttgcgaagct taatgttatt tttcttaaat aaggaatgat
     3661 ttctaatgag aaagaaaccc gaggataaaa aaacgacggg caaatatgca tcaatgtata
     3721 tgccgatcgg aatgtgcata ggcataggaa taggaatgtg tttgggaaat gcaatttttg
     3781 acaacttagc tatcggaatg agctttggtg ttggcgtggg actttcactc ggctgcgctt
     3841 acggtgcatc acttgacaaa aaggcattga atgttgttga aatcattgag gatgatttcg
     3901 gctgtgaagg agttcccgaa gatgccgagg ctacggtgac ggttgttgta accgacgctg
     3961 agggcaagga acagcgcatc aggatggcag ataagctctg ctatgaaaga aatatcgagg
     4021 caggagattc cgttatgctc gataaggacg gtacgctcaa gcagatttac aaaatcccgc
     4081 cgaaaaagaa aaaataacag ttaacaggga actgccatgt gcggttcctt ttgtttagcc
     4141 gataaataat taaaatgcgg catcccgcag cgaaacgaaa caagggcaca ttttctgcaa
     4201 gaaaagtgaa aagagtcggc agggagtctc tttgattgat acggatataa tactgtaaga
     4261 caacaaatct aacctattac agaaagcttc cgtaaataac aaaataatat caaagaaaaa
     4321 tattgacctt ggcacagaaa aaaagtataa taataaagtt aaatagaagt aaacgtatgt
     4381 attatttaaa aactgaatta tatcacagat aattcaatcc gagagctaaa atcttaaatt
     4441 taatatctgt tttgaaaggg ggcaggatga tggataaata taagcgagac cacagcgtta
     4501 taaaactttt gcaaattgct tcggcgatca gctttattct ttgcgtcgtt ttcatcattt
     4561 tgttgattct ttcaagaaac agcgatatac agctttggta ttcgaggtat cttgaatatc
     4621 ttgcttcggc agaatatagg gttgagcata tgagtgaaaa attttctgtc tttttgatcg
     4681 taatttttct ttatgcgttc aaggcggttt tcccgattta cctttatccg gtttcggctc
     4741 tctgcgccgt aacaagtgcc gtgttcccgt cgtatttttc aattccgata aatctttttg
     4801 gactttcggt tctttattct gttaaatatt tttggggcac aaaggtcggt gcaaacggag
     4861 ttcagaacat tctccaaaaa agcgaaacag tgcgttatct cgttgaacgt gacggcaggg
     4921 gaaacccttg gcttttggct ctcttccggc tcgttccggg gattccggta aatcaggtca
     4981 gcaagcttta cggcgcaatg gggtttaaat atgaatattt ccttttgctc tctcttgtcg
     5041 gttattcgcc gctgctggct tcctacacgt tcatcggtcg taacgtgttc aacccgttat
     5101 caacggcgtt ccttttgccg tttattttac tgttcctgtt aatgggaata tcaatgcttg
     5161 ccataagcaa aatagttcag atccagtcaa ggaggagaaa aaacaatggc tgatgcaaaa
     5221 acattgaaat caaggcttgc ttcgggcgaa ttcgacgcaa gattaaaaga ggtctatctc
     5281 agcgataaag ctgttgaaga tcagaaaaaa agagatgcgg ttataattga tgagttcgtt
     5341 cgtcttttcg gcgataacga ttcaattgag cttttcagtg cacccggcag aaccgaggtc
     5401 ggcggaaatc ataccgatca taaccacggc aaggtgcttg cggcaggcgt aaaccttgac
     5461 gtaatcgcaa ttattgagcc tgttgaggca aacaagatcg tnnnnnnnnn ncctcgatac
     5521 ggttgccgct gcggcaaaga gagacgacgg aatcattgtt gagaaatctt ttaagtttga
     5581 tgctcttgag gttgatatat ccgaccttga ggtacatacg gaggagttcg gaaaatcctc
     5641 gggtcttatc aggggaatgt gtgccggctt taaggaacgc ggatacaata tcggcggctt
     5701 caatgcagca agcatgagcc gtgttctctc gggctcgggt ctttcgtcat ctgcggctta
     5761 tgaggtgctt atcggaacga ttcttaatca tctttataat gacggcaagg tttcacccgt
     5821 cgaggttgcg aagattgccc agtatgccga gaataaatat ttcggcaagc catgcggact
     5881 ccttgatnnn nnnnnnngca agctctgtcg gcagcttcat cactattgat ttcaaggatc
     5941 cgtcccggcc gattattaac aaggtcgatt ttgactttgc ttcctgtggc catgcgcttt
     6001 gtattatcga cacgggtgga aatcattccg acctcaccga cgattatgcg gcggtaagag
     6061 gcgagatgga aaaggctgcg gcagtgtttg gcaagagcgt tctccgtgag gttgacgagg
     6121 atgagttcat ggataatatt tcgcttgtcc gtgaaaaggt caatgaccgt gccgtgcttc
     6181 gcgcaatgca tttctacggt gaaaacagac gtgccgaagc tgaggtcaag gctcttgagg
     6241 acggagattt tgaaaagttc aaggagcttg taacagagag cggtcgctcg tcatatatgt
     6301 ataatcagaa tgtctttacg tcaaaggacg ttgagcatca gagcgtttcc gttgcacttg
     6361 caatgtgcga atatcttctc aagaacaagg gcgcatggcg cgttcacggc ggcggatttg
     6421 cgggaacaat acaagcattc gttccgcttg atatgcttga tgatttttgt gagaagatgg
     6481 aagctgtttt ctcgaaaggc tcatgctatg ttctttctgt tagacctttc ggcggaatca
     6541 aactgtttta agcaattttc aggcgtgtaa actcgggttt acacgctttt ttcagaaaag
     6601 acaacctaag ttgatagaca gagcataaaa aattgatata atgctcagtg taaaacaact
     6661 ttttttaata ggtcgaaagg ggtaatacaa tgaatatatc cgaaaaaata cagatcttgc
     6721 gccgtgacaa ggaatggtca caggacgagc tggcggagaa gcttaatgtt tcacgtcagt
     6781 ctgtttcaaa atgggaatca ggaaaagctt tgcccgattc ggaaaaaatt cttgcaatgg
     6841 cgaatctctt tgacgtttca acggattttc ttttgaagga tgaacaggag ccggtttttg
     6901 tcgatgatga gaagaaacag acgaaagata aaacagacgg cgttttgaca gagaacacag
     6961 aaaagaaaaa gcataagttt tcaggaaaaa agattgtcgc aatcgttgtt gtcgcatgta
     7021 ttgttattgc tgcaattaca ccgctttttt tcggcggata ctcggcattt ctctcaaaga
     7081 taagcgaaga tcccgtacag tacccatatg tactcgtgca cggcctcggc ggctggggtc
     7141 ccgaatctca gattgaccaa acctcgccct attggggcag ctcaacgggc aatcttgccg
     7201 aatatctcaa ttcagagggc tacagcgtaa gcgttgcttc ggtcggtcct ttttcaagca
     7261 catgggacag aacctgtgag ctttatgccg agctgactgg cacaaaggtt gactacggtg
     7321 aggcgcacag caaggaacac gggcatgaaa ggtacggcag agaatacact gccgaaaatg
     7381 ccattgtaca gaactggggc ggcaagacaa aaagaggtca gcgtattaaa attaacgtca
     7441 taggccacag cttcggcggt gagaccgtaa gactccttgc ctcactcatg gcgtacggcg
     7501 atgaggcgga aaaggcggct acaggtaagg atacctccga gcttttcacg ggcggtaagg
     7561 ctgattggat aaacagcgtt acaactcttt gctctcccca taacggttca acgcttttct
     7621 atgttgtgga tcagggtaaa ctcgtaaata cggttctcgg ccttgtttat gccgcaagcg
     7681 gagttgcaaa aacggctcag ataggcgatt tttatgattt tcgccttgaa cagttcggct
     7741 tgaacggagc ttcgagcgaa agtcaggtgg aatcgattat aaatacggtt ttcagcgagg
     7801 gaaccgataa tgccgcatac gatctttcac cggacggagc acaggaattg aacaagaaaa
     7861 tcaagcttgt tgacggcgtt tattattttt catattcata tctcacaacc gaaacgagtg
     7921 ctttgaccgg caaacaaatc ccgaaaagct caacgcttcc tgtcctgatt attccggcaa
     7981 cgctcatggg tagatattcg acaaacacca aaacggattt caagattgac gaaacatggc
     8041 ttccgaatga cggacttgtc aacgttgttt cggcaaggta tccgatcggc gacgaatatc
     8101 aggaatatga cgcagagaat atcgtaaaag gcaagtggaa cgttatgccg accctgcccg
     8161 gcgaccacgg aacggttatc ggtatgaatg tcagtgcaga ggaaacgcac agcttctacg
     8221 acatactttt taagatgatc gactctcagc cgagagataa gaagtattat attttttaat
     8281 gaggcgaaca aatgaaaatt ctttttaaaa atgcagatat acttgtaaga gatgaaaaat
     8341 tcaatgtgct ttcaaacgct tttcttgccg ttgacggcga taagataagc tacatcggtg
     8401 cggaagagcc gaaagacgaa tttgacgaaa taaaggacat gagcggtaaa ttgctcatgc
     8461 ccggtctcat caactgtcac aaccattgtc cgatggttct tcttcgcgga attggcagtg
     8521 atctgccgct tcagcagtgg ctgtttgata tgatatttcc gattgaggac agactcacgg
     8581 acgatgaaat caaagcagga agctaccttg cgcttttgga gatgattgcc ggaggaacgg
     8641 tcagcttttc ggatatgtac ggaccgcaaa tgaatgttga ggctgtcgga gagtcgggta
     8701 tgaaggcaaa tgtcacccgt cccatcctgt ctgccgaacc gaatgaaaca cccgagcaat
     8761 catacagaat gagagaagcg atcgagcatt acgaaaaata caataacacc tttaacggaa
     8821 gagttttgat tgattattgt attcatgcag aatatacctg tgacgagaga acggcaaggg
     8881 tcttgataga cgagatgaat gcacacaacg gaaacctgca tattcacctt tcggagacga
     8941 aaaaggagca tgatgagtgt attctgagac acggcaaaac gcctgccgag tggttcgaca
     9001 gtctcggcgc atttgagtcc tcggcttttg ccgctcactg tgtatggctg agtgaaagcg
     9061 acatggagct gttccgccgc aagggagtta gcgttgttca taacccgacc agcaacatga
     9121 agcttgcaag cggctttgcc cctgttccaa aaatgcttga tatgggtata aatgttgcac
     9181 tcggtaccga cggcccctca agcaacaaca atcttgatat gttcgaggaa atgcacatcg
     9241 cttcgataat tcacaacggc tacacgcttg acgcaacggt tatgaacgca gatactgttc
     9301 tcaaaatggc gacagtcaac ggtgcaaagg ttcagcgcag agagaactgc ggcgagctga
     9361 aggtcggcaa taaggcggat attatcgctg tttctctcga cgcacctcac atggtgcctg
     9421 cactcgacaa aaaggcgctg ctgacatatt ccgcacagag cagcgatgtt tcaatgacaa
     9481 tggttgacgg caagattctt tatgaaaacg gcgagtataa aacactcgat aaagaaaaga
     9541 tttattttga gttcaacaag gctgttgaga agctttataa ataagaggta gcacaatgga
     9601 aagaatggac aagggcttgg cttttatgct gagatatgaa aacgttgcat ggtacgagaa
     9661 cggcgaggtg agaattcttg accgccgaat ttacccgaga aaggtcgagt ttgtgacctg
     9721 caggaatcat cacgaggtca ctcaggcgat taaggacatg gtgacacaga gcgcagggcc
     9781 gtacactgcg gcggcaatgg gtatggcact tgccgcttac gaatgtaagg acaagtcaga
     9841 agcggagcag atcggctacc tcaccgaggc ggcatatacg atttcgcacg cacgcccgac
     9901 caccgttcag cgtatggcgc tcatcacggg cggatgcctc gatgcggcaa agaaagcgat
     9961 tgcggcaggg gaggacgtca gcgaaaaaat cgttgagcac actattgaag cgaacaatct
    10021 ccgctacaaa aaaatcaatc tcattgccga acatctcgtc gatatgttcc ctcagaacgg
    10081 aacggttatg acccagtgct tcggtgaaac gatagtcggc atgatgctca aggtagcaag
    10141 agagaggggc aacaacgtca gaatcttctg ccccgagaca aggccgtatt tccagggcgc
    10201 aaggctgacg gcgacggttt gccatgagat gggctttgac acgaccgtta ttacggacaa
    10261 tatgcctgcg tttgtaatga agaaagaaaa tgttgatgtg ttcacctcgg cggccgacgt
    10321 tatctgcatg gacggccacg ttgtcaacaa ggtcggcact tatcaaatcg ctattgcggc
    10381 gaagtacacg ggaattccgt atttcgtaac gggtgcgccc gacagaggac acacaacgat
    10441 cgacacggtt aaaattgaga tgcgtgatcc cgattttgtg cttcaggcta tgggagtaaa
    10501 gaccgccgca gacggactca agggatatta tccggcgttt gacataacgc cgcccgagct
    10561 tgtcagcgga gttgtcaccg acacggggat ttacacaccc tatgacctcg aaagctattt
    10621 taaaaagggc acaatgggcg agtttgagat gatattgtaa tagggtaaat gaacttggcg
    10681 cccctttagg ggagctgtca gcgtagctga ctgaggggtt ttgtaggaag tttgctgatt
    10741 tttaaattaa cccctccgac ttaggcgcaa cccgccgaag tcacctcccc tgcatgggag
    10801 gcaagatgct tttctgcacc ggaagcaata ttcatcagac tctcttggtg cgatgtgggc
    10861 atcgcaccct acgatgatgt gcaaaaaccg aacaattcgt aggggtcgat gcccacatcg
    10921 acccaaggtc aggaaaaatt gactaaggct tccccttgag gggaagctcc gtcgtaaggc
    10981 ggtgatgagg tggaaaaagt cgagtcagat tttgttaagt aatgagatga aaaattgcca
    11041 cctcatccga gcggcgcaat ccgccgccca ccttcccctc aaggggaagg ctgttaaaat
    11101 gtctgcaaaa attttcatgg agcgtgggct cctcctccgg gggagctgac tgagggagtg
    11161 aggtttcatc agacttaaac tccttgacga
//
LOCUS       DAWBSW010000052        11126 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1261_length_11126_cov_2.462560, whole genome shotgun sequence.
ACCESSION   DAWBSW010000052 DAWBSW010000000
VERSION     DAWBSW010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_11126_cov_2.462560"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    526..593
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2516..2525
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3929..3938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4794..4803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6184..6193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3206 a         2273 c         2389 g         3150 t
ORIGIN      
        1 gaacaacgtt gagcgcaatg gcgacacagc cgctgagaat gccgtatttc attcttactt
       61 tggagtcggt aacgttttta tagtctttta taaaagtttt gacaataata tttgtcattt
      121 aatcactcgt ttcatttgtt tatatgcaca tcaagctgat cataggggat atgtattccg
      181 tttttgtcaa aagcaagctt gactttttcc tgcatactga aatacgcagg ccaataattt
      241 ccacttttac accaaacgaa gcaggcgagg tcaacgccgc ttgcaccgtg ttttgaaaca
      301 ccgatggtat gcttcggctc cttgagaaga agcggttcgc tctctacaac ctgtttaagc
      361 accttcttgg ctttttcaac gtcgtcgtca tagcttatcg agtaggtcag gtcaagtctt
      421 atcttgtccc ttgcagtgta gtttgtgagt gttgaagtcg taattttctg atttggtaca
      481 actacaagct tgttatcgtt ggtgacaagc gttgtgtaca taaatnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgaacc
      601 tttccgctga cgttttcaat ctcaacaaaa tcgccgctct tgaacggttt gttgaaaaga
      661 atctgaatgc cgcctgcaag ctgagcgatg ctgttttgca gtccgaggcc tgccgttatt
      721 cctgccgctc cgagggcggt aatgaatgaa tttacgttaa agccgacctg ttcgagagca
      781 acgacagcga caatgattct gagaaaaatg acagttgagc ggtttacaaa ccggtgcatt
      841 gacttgtcaa cgttcttgct ttcgagtatt ttaaccatga gttttccggc aagcttcgac
      901 agccaaaaac cgacggcgat aacaacaata aaaacaataa gctttggcaa tgcggcggca
      961 aatttttcgg gaaagttcgc aaagctccat tgcttaagct cattcaaaaa tgaattcata
     1021 gttttcctcc gtgttatttt attattcgat agtttatttg ggccgagtgg gcatcgatcc
     1081 ctacggattt tacatatacc aatccctgca aaacacattc acactcaaat tatactatct
     1141 gtctgccgta attgcaagcg aagaaaggac tgccgcacgg gcgacagtcc ggaaaatttc
     1201 gttttagatt atgccgagaa gatttgcaat cttttcaaca aggaattgaa taattccgaa
     1261 gctgtgagac tgaatgagcc tcgtgaactt atcgccgagc ggacccgtga agagccaagg
     1321 catctcagga atatcctttt cttttgcctc tctgccgtca taccgaagct caaacgcctg
     1381 cgaatatgtt acgccgccct cacccataag ctcaaagcga atgtagcagc cgagctgatc
     1441 ctcataatcg ttgaggtcaa tctttgtatc tgtagaaatc accttaccgt cggcaatcca
     1501 cttaacggag ctgcaatcac gtctgctgtc aagcgtaagc tcgattgtgt tgtctttttg
     1561 gtcaaccttg atgtttgaaa caagaggaac ctcaccgttg ccttcaaaac ccggtgtgcc
     1621 gtctgtatat ttttggaatt tgctgcaagc aaagaagttg ccgacctgca tggcttgctt
     1681 gacgttttcc tctgtgtttt cggggagaag gaagtattca aagcttctgc ctacgtcgga
     1741 tacgtactcg ctgtcgtcat cggcaaatac gatgatgttt ctgcctgtgg ggataacgac
     1801 ctgaagaagc tcatcccaaa gggctctgtc ggctcttgtt accgagtcgg tattgttgat
     1861 aatctccata ccgaggcagc tcttataatc cttaaaaata tttgcaaaat aagaaatgta
     1921 aaaatcctga tgtgagcgct cgggaaaatt attcgaatta acccagtcgc cgacatggtt
     1981 aagaacagtg aaaccgccgt aacggtcaac tgcggcaacg gaagaacggt agtcgttttc
     2041 gttgccccaa acagcctggc catagtcggt gaaatagccg ttcacatgag tcttggtcat
     2101 aacagccatg ttcatttcaa ttccgcgcgt gatgtcgatc atacctctgc cgtttctgtc
     2161 ggaaccggtt gtgattctca aataatcctc atcgctcatc ggatcggctt tgcggaaata
     2221 gttaaaaatg ccgtttgtct gtctcggctt attccagccg tagttaataa caccgtgatc
     2281 ggtcatcgca agaatatcat agtcgagact gtaataaagc tcaaccatat ccgtaatact
     2341 gagatcgccg tcggacgcaa ccgaatgggt gtggagattt gctttgtagt aatcccactt
     2401 atcccagtca acgcagtcat acggattgac tatcgtgtag tcggtttctc ctaccgcata
     2461 ggcggacatg agaggggaga acgctgtgaa taaaagagtt gcgcaagtga tgacannnnn
     2521 nnnnnttaag acctgccgac gcaaatcaaa aaaacttatt gcaacacggc gaaaacaggt
     2581 taaagaaaga cgtgcacaag aatttcagag gtgctatctt agcttatttt attctgaatt
     2641 gaagtgcgat tccgccgaaa gaaacaacgt ctccgtcgtt tacaacggta ggctcttcaa
     2701 tacgtttatt atgtacatat gtgcctgaac ttgaatgagt gtctgtaata atccagccct
     2761 ttttatgctt ggatattaca gcatgaaaac gtgagaccga ggcataattg agcacaatgt
     2821 cacaggcgtt gctcctgccg atcgaggtct cccagcttgt tataacatat tccttgccgt
     2881 tttgctcatc gtagaaatac gccggcgtga ccttgggcgc acggctcgaa agcaaatgtt
     2941 tgacgcaata tgcggcaatg atgatgcaaa gggcgggaat aagatatctg taaatttcat
     3001 ttaacagttc tttcattgaa tcatcctttt aatgtttaat tcaattataa ggtttgcaca
     3061 aaaaaaagtc aacatttttt gctttaaatg ttttcagtat tgtgatataa ttatgcaaag
     3121 ctttaaatgt acaaacggag gtgcattttc aatgaacatt gcaaaaagga ttttagctgt
     3181 attcttaacg ttgacaatgg cggtgacgct gttcactttt tcaacgggag cgatcgaatc
     3241 acagcaggaa tggattgaaa aaaactggtc tcggcttttg gagtcaaaag gatcattgct
     3301 tatgacgccc ggcgaggata acacaaagat gaacttttca tggcagtcgt cctataagag
     3361 ctgtaaggct gaaatcgagg tcggaacaaa gcaggatatg agcgacgctc aaaagcttga
     3421 tgttgacagc aaattcaata ttttctgctt tgaatatacc catgaagcaa cggcgtcgga
     3481 gcttgaagca gacacaacat actactacaa gtattatgcc gataaggttt ggagcgaggt
     3541 ttactcgttc actacggctt cttccgaaag cacaaaggtt ctgtttgttt cggactgtca
     3601 gataggacgc ttccgtcaag gaacggatga ggagatactt cagcatgaca cttacggctg
     3661 gtacaagaca atggagcttg caaccgaaag tcacaagggc atcaatttcg tcgtttcagc
     3721 aggcgatcag gttgaggatt cctattccga gaagcagtat tcgcttatgg aatcggtacc
     3781 ccagcttaga aattatccga tagccgctgt tgtcggaaat catgaattct ataccactaa
     3841 ctattcaagt cactacaata acccgaacag agataccgag gtgccattta agaaccctgc
     3901 cggaaacgga tattatttcc tatataacnn nnnnnnnnat gcatcacagc ccgtatgatg
     3961 caaatgcagc caagtatttc acaagtaaaa tcacaagagc aactatcacg ccgttcttcg
     4021 atgagttcgg aatcgacctt tgtctgagcg gtcatgacca ttactacagc cgttcttata
     4081 ttatcaagaa caacagggta accgacgacg tgcttacaaa caatacctac acaaacccga
     4141 agggtactct ttatgtcagc gcaaactcct cctcgggcag taattataac ggaatagata
     4201 ccgaaaatgt aggaccggaa tgtgatttct ggttccagtc cgatacgcct tgctactcaa
     4261 taatggacgt ttccggcgat aagctcagcg taacgacata tgaaacggat aacaacagtg
     4321 tagtagatac aatctcgatt gcaaaaaaat aagactgaaa tcattaaaag gactgccgat
     4381 cacaaaccga cagtccttgg ttttttttga ttttattttg tagcttcacc gatttgttgt
     4441 gcacagtcct tgcagatttt ctgaccgttg aattcaatca aatcatcggt ttcatgacag
     4501 aaaatacagg agggagcata ctttttcatc aagatttcat ttcctcttgc aaggatctca
     4561 accttgttgt tttctcccat tatatcgagt tcttttctga tctcgatagg aagaacaatt
     4621 ctcccggcgt tatcaacgct tctgacgatt cccacgctct tcataaatac gactcctttc
     4681 ttaaaaatcc gaccgcataa gcggcttttc tttatttgat tacataatac cactttttac
     4741 catattagtc aacacatatt ttaaaaaaaa taagcaaccg caaaagcagt tgcnnnnnnn
     4801 nnngattcag gtaattattt aatcaaacct gtaatgaaat cgataatctt ctgaaggatt
     4861 gcctggaagt caatctttga aagaatatct gtaatctttc caaggatagc ctgaatgtcg
     4921 atcttatcaa aaattgtgct gaaatccata actcgttact cctttcgtag attcggtaac
     4981 agaaccaatc tgctatctgt gttgtatgtt agcacatttt tgcttaattg tcaatacacg
     5041 gataagtatt ttttgcgacg gttcattaaa tatataaaaa agcagtgaca acgttcattg
     5101 ccactgcccg ttttggttat aaacaaataa caaattataa tcaaattaac ctttgttaat
     5161 aaagcccata acaagatcga taattttatt aagaatcgcc gatacgtcaa tctttgtaat
     5221 gagatcaaca agcttgttca taactgtgtc aagaatcgca ttgaaatcca taacttgtta
     5281 ctcctttcat aaaataaaag tgtagaggat agatcttcta tctgtactta taataacaca
     5341 ttaatttatg ctatgtcaag gaaaatacct ttataattta caattattgt tgattttggt
     5401 ttaatacggc tgtaaaaaat tactgtttta ttcatctttt tacgtagaat tatttcaata
     5461 atgcatattt aattatattg agcgtgtttt tcctgaaaaa aattgcaatt ttgccgttgt
     5521 attgaagaaa actattgtca attcgattca aatgtcttat aatctaaaca aagagaaaaa
     5581 aggacgtgtt ataattgaaa aaaactatta ctgtttgtat caccgttatt atctgtgtcg
     5641 ccatgatgtg cggctcgttt gtttatgtag gcaaggattt taacgagagt ataggaaagc
     5701 ttatcccgtc gattcttgtt tcttcgagaa taaacaacga taaaaaagaa cccgaaacag
     5761 ttcccgcaac cgttccggca accgaaagca cgaccgagcc ggcaaccgag gagactacgg
     5821 gcgagatgac gacgagcgaa agcacaacgg gcaagcagaa taatgtaaag ccgaatacga
     5881 ccgcaaagcc gagcacgaca aaaaaacaga ctactaccaa aaaggtaaca acgacaaaga
     5941 aggttaccac gacaaaaaag gttaccacaa caaaaaaggt tacgacaacg aagaaaacaa
     6001 cgacagctcc gaaaaagctt acaacggcgc agataatcaa taactacaac gcagccgtga
     6061 ataaggtaat cagctcaaag gcaggttatt ccaagacaag aagcacaaag ctcaacaagc
     6121 ttgatgcagg cgcactcagc aacatgggcg tcgttaagga tgctgtttcg ggcttcctcg
     6181 gagnnnnnnn nnntcctcgg agtgggcgac aaaacctata caaacgcaaa gggcaagagc
     6241 gaatttatgt caaccgcagg acttaaagcc gccgatgtca agagcgcaag ctacactctc
     6301 agcggcggaa aatatacata tacacttgtt ctcaataacg gttcaagcta cgccgattca
     6361 gcaaacaaaa agaataattc gcctgttgac aggagcggaa tcctcgtcgg aacgggcgat
     6421 aaatccgcat tcgatcacaa gtgcgccgca aatctttaca cagccatcaa caataccgac
     6481 ggcgcttcgg ttaagtctgt aagagagtct tcgtcaaacg ttaagtgtgt tgccgttgta
     6541 aacgcatcta acggcagact cgaaagactt accgtttcat ttgattttgc cgttacgctt
     6601 accaacacaa agtatgttgt aacaattaag aacgcaggcg gaagcgcgtc aacatccgtt
     6661 aagtattccg gcttcaagtt ctgagcaaag taaaataatc gaccgctctc aaacgagggc
     6721 ggtcttgctt tctgtgcaga ttatgcattt gtgtcaataa cggcattgca gatttccggc
     6781 gttatgaaat cgaatacggc gatgccgagg tgagcctttt tatccttgat attcttcata
     6841 aattccgcat tgacgtgctt cgcaaccctc agaacatggg gatatccgag aacgccgctg
     6901 tgaaaattta tgctgagatt tttcttgccg ttgctttcct tgaggatttt gttaaaatgc
     6961 tccgtaacga gcttccacgc ctcctcaacg ttttcaagct tgaatttatc ctgaatgtgc
     7021 agtcgaaaat cgccgtgctc gattaccgat gtgccgttat gtacccagtt ttcggaagcg
     7081 tcgattccga ttccgagatt tttgacacgg ttaagaagaa ccgcttttcc tctgacctcc
     7141 gaaagcttcg gcaggcgatt ctctgtaaac catatatcac cgtgagcttc aagctttttc
     7201 ataaaaatag aattgaattc attctttttg tctcttgaaa taagctcatg cttgacggat
     7261 ataattatcg tctcgctcgg gtttttgcga agaaaatcaa agcactgttc cgttaccttg
     7321 tcaaatgtga ttccctgaaa gatcggaccg tgggagattg caagatcacc tttgacaaca
     7381 ttcgctcgga tatcaagata acgaactccc atattaagct gatcggtgac tgtatacatc
     7441 tggcacttgg caagctcttt gagcggttca tacagagcac aggtatcgtg tgatccggga
     7501 acagaaattt cgtttatata tttttcaccg tcaagacggg acatccagtt gtttttatca
     7561 aaattgccta tatccttgtg cacatagttt ccacgaagcg cttttatcca ggttaaaaag
     7621 gggaatgagg gtgattttat ctccgacata gcattattta ccttcttttt ggttagaaat
     7681 attgtaaaac acataaaaaa atcaaataac ggtttatttt atttataaat aattaaagtc
     7741 aaatgtaaat aaaaattgaa tttttctttt ttagcttgca taatttccga aaaaaatgta
     7801 ttatatatgt aaataattac atttattata aggaaaggtg aggcttttga agaaagattt
     7861 aatactcaaa aaccgaaccg ctgtcacgga gaagatcaag gaatcggctc gctcagttct
     7921 cccgatagtt attatcgtta cattgttatg cttcagcatt gcgcctatta acacagacct
     7981 gatgctgaat ttttttatag gtacagtgat ggtggttatc ggtatcggtc ttttctcgct
     8041 cggtgcggat acttcgatga tcaaaatagg aaacaagata ggaaccgcgc ttacaaagac
     8101 aaagaagctg ccgctgattg tgcttgtcag ctttgcgttc ggctttgcgg ttacaattgc
     8161 cgagcctgat ttgcaggtgc ttgccgagac ggtcccgcat attaacaata cggtgctttt
     8221 gataacggtc ggcgtaggag tcggattttt tcttgccgtc tgcatgatga gaattctgac
     8281 gggtctgaag atgaggtggc ttttgattgc attttatgcc gttgtattta ttcttgcggg
     8341 attcacggac gatagatttt taagcatcgc atttgactcg ggcggggtaa cgaccggtcc
     8401 gatgaccgtt ccttttatcc ttgcgctagg tgtcggcgtt tctaatattc gaagcgacga
     8461 taaggcggag gcggatagct tcggccttgt tgcgctcagc tcaataggtc ctattcttgc
     8521 cgttcttgtg ctcggattct tttatcccgg cggggacacg gttaccgaag tcagcgttct
     8581 ttctttcggc agtacgggtg aaataggcaa gatctatctt accgaattgc cgagatatat
     8641 gggcgaggtt gcactttcgc ttttaccgat tgtcggaatt tttttattgt tacagctttt
     8701 gattttgaag atttcaaggc gcagtcttgc aaaaatctgc gtcggacttg tttatacata
     8761 tgtcggcttg gtgcttttcc ttacgggcgt caacgtcgga ttttcttctc tcggctctgt
     8821 tctcggagcc gcattggcag aggattggac gaagtatatt atgataccgc ttgcgatgat
     8881 tttcggctgg tttataatct ccgccgagcc tgccgttacg gtgcttgaaa atcagatcga
     8941 ggaggtcagc gccggagcta tcccgggcaa ggcgataaag cttagccttt ccgcggcaat
     9001 tgcggttgca atgggaattg ccatgctccg ggtaatgacg ggaatctcaa ttctctggtt
     9061 ccttgttccc ggttatacaa ttgcacttat tctgtcgttt tttgttcccg atatttacac
     9121 ggcgatcgcc tttgactcag gaggagttgc ctccggtcct atgacggcaa cgtttatgct
     9181 tcaattcttt atcggtgcga gcattgctct gggcggtgac gtgctcaaag atgctttcgg
     9241 tgcggttgca atggtggcga tgatgccgct gataaccatc cagacggtcg gtattttcta
     9301 tacaataaga gacagaaaag aggagaagca cgccgctgcc gaattcggcg attacgatat
     9361 tgttgagctt tgggaggaga gtgccgtatg aattttgtga tttcaatttg caacccggac
     9421 aatgtaaata ttttaaaaga gatctgtcac gagctgagtc ttcctataaa tgtcatactc
     9481 aggggcaggg gaacggcggt aagaagtatg cttgaccttc tcgggcttga aagcaacgaa
     9541 aagcgtgttc tactctcggt tgcggataat gaaaagctta aaaaatttat caaagagcag
     9601 aagaaaaaaa tgtttatcgg tgtaccgggt cacggaattg tcgtatctgt gccgataaaa
     9661 agcataggag gtaaaagaac gatggaaaag cttaatgccg atgctcagct tatcgggacg
     9721 aaacccgaat ttaatccctc atatgagctc attgtcgcca ttgcaaacga gggcagaatg
     9781 gataacgtta tgaatgccgc aagagctgcc ggcgcaacgg gaggaacggt tctgcacggt
     9841 aagggagagg gcggaagaaa tgaggaaaaa ttctacaatg tttcaattgc aagcgagaaa
     9901 gaagttatcc tcatcgttgc aagaaaggaa caaaaatcgg atataatgcg ttccattctt
     9961 gaaaaagcgg gaccgaatac ggatgcggag gcaattgtgt tttcactgcc gacgagcgag
    10021 gtcgcaggat tcggaatgtt tgacgatata aacgaataat attaaacgcc gtgattttat
    10081 acggcgtttt taaaatttta tattatatac ttgatttttt tattctgttt ggtataattg
    10141 aaccatagtc caaataaaag gagaaaaaag caaatgtcaa gaagatacga caaggaaggt
    10201 tctaagagaa gaattttatc cgtgtgcgta aaactcttta tcgaaaaagg atttaaaaaa
    10261 acaacaaatg cggaaattct tgagcttgcc gacgttacaa gcagcacatt ctataatata
    10321 tttaaaacaa aggacggtgt actccttgag ctgaccgagt ttatgtttga aaatcagttc
    10381 agcatagccg aaaagcttgt aggagagggc gcgtctccca ttatggtata tgccgcagag
    10441 actgccttgc aattaactct taccgagctt aacgaaaacc tcagagaaat atatgttgag
    10501 gcgtattctc agccgcacat tgcggagtat atttaccaga atactacaaa tgagctatat
    10561 aagattttta gttcgtatat gccaaactgc tcgctcagtg atttttatga gcttgaaatt
    10621 ggctctgcag gaatcatgcg aggctatatg tcaagaccgt gcgataaata tttcactatt
    10681 gaaaagaagc ttgaacgctt tatcagaatg agcctgagcg cctataacgt tccgaaagag
    10741 gaacaggata aggcagttgc atatgtgctg gaatccgaca taatatcaac cgcaaatgca
    10801 gttatgaaaa tgctttttga ggctctttcc atgaaatttg atttaactct aaatcaaaaa
    10861 gcgaaagaaa tttcaggtta atcagaatct cggttttatt ttaaagaatc aaccgccctc
    10921 agattgagag cggttgaatt gtttatgtat taaattattt caaaaatctt ccgacggtgt
    10981 ccgtatcgct gtagaatgtg ctgaaaaggt ctccgccgac ttcaaggaag ttttcaaatc
    11041 tcgggccaac gttcatgccc atattgaatt caacattaaa cttcgccaat gcaccgaagc
    11101 cgggatagga tttctgcttc aggata
//
LOCUS       DAWBSW010000053        11074 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1271_length_11074_cov_2.549052, whole genome shotgun sequence.
ACCESSION   DAWBSW010000053 DAWBSW010000000
VERSION     DAWBSW010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_11074_cov_2.549052"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4022..4031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4790..4799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2768 a         2798 c         2579 g         2909 t
ORIGIN      
        1 ggcctcattt attatgattt tatattcttg ctacacccga atcacgagcc gcctgagcaa
       61 cagccgccgc aacggtcttg ccgacacggg gatcgaaagg cttcggaata atataatctg
      121 cggaaagctc ctcatcggaa acaagacctg caagcgcctt tgccgctgca atcttcattt
      181 cttcattgat atcgcttgca cgaacatcaa gcgctccacg gaaaattccc gggaatgcga
      241 gaacgttatt gatctggttc gggaagtcgc ttctgcctgt tccaacaacc gccgcgcctg
      301 ccgccttggc aagatcgggc atgatttcgg gaacgggatt agccattgcg agaacgatcg
      361 ggtccttagc catcgacttg atcatatcct cggtaacaac tccgggagcg gaaacaccga
      421 taaagatgtc tgcgcccttc attgcatcgg cgagtgaacc tctgaggtga tttttgtttg
      481 tgaagtctgc gatatcctgc ttagccggct tgaggtcgtc accatcgcag attatgccct
      541 tacggtcgca catgatgatg ttgcccatac ccatgcttgt gaaaagcttt gcaatcgcaa
      601 cgcctgccgc acctgcaccg ttgaatacgg ctgtgcagtc ctcaatcttc ttgccgacga
      661 gcttcaaagc attgataatt cccgccgaac agataactgc ggtgccgtgc tggtcatcat
      721 ggaaaatagg aatatcggct ttttccttga gctttctttc aatttcaaag catctcggag
      781 cggcgatatc ctcaaggttg attccgccga agctgccgca aagcagagca acggtgttta
      841 caaattcgtc aacatcctgt gtcctgatgc aaagagggaa agcgtcaaca ccgccgaact
      901 ccttgaaaag aacgcacttg ccctccataa cgggcatacc tgcctcgggg cctatattgc
      961 cgagaccgag aacagccgaa ccgtcggtga taacggcaac gagatttgaa cggcgtgtaa
     1021 gctcatagct cttattgatg tccttctgaa tttcaagaca cggctgagca acgccgggtg
     1081 tgtaagcaag cgaaagggca tccttgttat cgacctttgc tcttgcgatt acttctatct
     1141 tacccttcca ctcataatga agcctcaggg attcctttgc ataatccatt atacttgtcc
     1201 tccgatcaaa ttattatatt tctcccacgg gaatacatag ttaagcttga ggtcatcacg
     1261 aaccttgttc atttcagcct gaacggattc gtttatcggc acgccggaat cctttctctg
     1321 aagccaaacg ttgtactcct tctcgcctgc cgagaagata tggtcgtgac ccggtgcttt
     1381 cttggatgcg cgaacggagc gaatgatctc gcctgccttt ttccttgtaa gctcctcgcc
     1441 gaggaagtga cctgtatcaa tggcaataaa gaaatgaccg agatgataag gtacaagatt
     1501 accgttttcg tccttgccgt caagcatctt gccgtagttg ccgtcctgga gagcagccga
     1561 aagaagctcg ataaccatag cgtagccgta gcccttgtat ccgccgagag cctcgccgat
     1621 accgccgagg gtcgtcaaag cggctgttcc gttacgaatc tttttgagag cttcgcctgc
     1681 gtcgccctca acgggattgc cgtcgatatc aataactgtg cccgggtgaa caggctcgtc
     1741 aattcttgca taatactcaa tcttgccgtt ctgcgtaatt gatgttgcgc agtcgataac
     1801 aaaattaaaa tcctcatcgg tcggaatacc gatcgtgagc gggtttgttc cgaacatacc
     1861 ctcaacgccg aaggtcggag caacagacgg acgggcattt gtacccgtga taccgataca
     1921 gcctgccttc gcagccattg acgcatagta gcccgcaata ccgtagtggc atgaattacg
     1981 aacggcaacc atgcccatgc cgtattcctt agcctttttg attgccattt ccatcgactt
     2041 gtagccgatg acctgaccca tgccgtcatg gccgtcaaca acagccgttg tcggggtttc
     2101 tttaataatc tcaaaattcg tgacaggatt ctgaattccc gcttcgattc ggtcgatata
     2161 aatcggcttg aagcggttgc agccgtggga ttcaattcct cgtctgtccg attcaaggat
     2221 aacgtcaacg acgatctcgc agtcctcggc aggaacacct acgcccttaa atgcgtcaac
     2281 cataaaatca tgcaatgttt tccaatcaca aacaactttg cccataataa aacctcctat
     2341 gtaaaataaa taatttataa attaagattg atctttatca atcaaaatcg tcctcctgcg
     2401 cccagttgcg cgaacgctca acggcacgct tccaatcctc gcagagctta tccctgacct
     2461 tcttgtccat tttcggaata aatacccttt caacctcacg gttcttttct atctcctcgg
     2521 gcgttttcca cattcctacg cagagacctg cgagataagc cgcgccgagt gccgttgttt
     2581 cgactgtctt tggtctgttg acaaccgttc tgatgagatc cgcctgaatc tgcatcataa
     2641 tattgcttac gcttgcgccg ccgtcaactc ggagctcgga aagaattata tttgaatcct
     2701 tcgtcatggc tcggagaagg tcggtcatct ggaagcatat tgattcaaga acggcacggc
     2761 agaaatgcgc acggttaaca cctcttgtaa gtcctacaat agttcctcgg gcatacatat
     2821 cccagtaagg tgcgccgaga ccggtgaaag ccggaacaag gaacattccg tttgcgttct
     2881 caacactttc ggcaagctcg tcaacacggg gcgccgtatc gaccatgtga agctcgtctc
     2941 tgagccactt gataaccgag cctgcgttga aggcactgcc ctcgattgaa tatgtggttt
     3001 taccgtttat gctccacgca acgccggtaa gaagattgtt ttccgaacgg actcgctttt
     3061 cgccggtgtt catgagcagg aagcagccgg ttccgtaggt gttcttcgcc tgaccgggat
     3121 tgaagcaggc ctgaccgaac aatgcagccg gctgatcgcc gattgcaccg cagatgggaa
     3181 caccctcaag ctcctcaata ccgataattc cgggggcaac tgtgccgtag atcttgctcg
     3241 attcaacagg ctcgggaagc atacacatag gtacgccgag cttttcgcaa aggaactcgt
     3301 cccactgaag cttgtcaacg tcaaaaagca tcgttcttga agcgtttgaa tagtccgtga
     3361 cgtgaacctt gccgcctgta aggttccaaa tgagccatgt ctcgaccgtt ccgaaaagaa
     3421 gttcgccgtt ctcggctctc tctcttgcac cctcgacatt atcaaggatc cactttattt
     3481 ttgttcccga aaaataagcg tcaataagaa gtccggtgtg ctcacggata tagtcgccga
     3541 gtccctcctc cttgagctgt tcgcaaagct cggcggtacg gcggcactgc caaacgatgg
     3601 cattgtatat cggcttaccc gtctccttat cccatataat tgttgtctca cgctgatttg
     3661 ttatacctat tcccgcgatt tccttaacat caaaatcacc tgaattgata acggttgaaa
     3721 ttgaatgcat ctgactgtag agaatatcca tcgggtcatg ctcaacccag cccgacttcg
     3781 gatatatctg ctcaaattca ttctgtgcct tggaaacaat gcgtccgcgc ttgtcaaata
     3841 ctattgcacg ggaactcgtt gttccctggt caagtgctaa aacatacata aaattttcct
     3901 ccctatataa ttaaggtact gccgcaccga ttgcggttca cgccttgctt gaatctttac
     3961 cctgccgcgc aatttgcacg actcaatttg tgcgggattg ctaaaaaaag aacagaaaaa
     4021 cnnnnnnnnn nctgtttcga aaaacagccg attttccgtt cattgaatac gtttcatata
     4081 ataaccgccg cccattttca ggcatttgca ctaaacgtgc agtgttgcct taatcaatga
     4141 tattgtacct tattttttct gaaaagtcaa cactgtttat cctcccaata tataaataaa
     4201 tgtataaatt caacaaaaat tcacggtttt cggcttgctt ctgaaactgt taaaaatcat
     4261 atatccagcc gtttgttttt ttgactgata aaaagctttt ctttgtcacc aaccttcctc
     4321 ttttccatac aatgttagtg taagacgaaa ggaggtcaga aacatgggtt gctgcaataa
     4381 ctggatcctt attctcataa ttatcctctt attctcagag tgcggttgcg gctgtggctg
     4441 cggaaacaac ggcacatcgt tcctcaacaa caacggttgc ggttgctgac attagctgag
     4501 aaagacgcct gcggacagat gctcgcaggc gtcgtctttc tgcattattg ttaaattttt
     4561 aattagttcc gtttctattg aatttgtcaa tttttcatag tataatacat tagtatatat
     4621 gaaaaaaggt ggattacttt gaaaaacggt gcaaaaaata aatttaacaa aaaatcgctc
     4681 attgccgtaa tactttgtgc cgtcattgtt ttcggcattg tttcatatga ctttataagc
     4741 ggcagaaact catgtgaaaa aacgagcgtt gccatgggta cggtcgtcan nnnnnnnnnc
     4801 ttcggtgcaa aaaatgacct cgataaaatc gaaaccgaga tcaacggtct cgaaaattcc
     4861 gttctaagct ggagaaaaaa aggctccgac gtttacagaa tcaacgaaag tgcaggtgct
     4921 tctgtttccg tttctcccga tacagccaaa atcatcgggc agtgcatcga catctcggac
     4981 gattgcggcg gcgtttttga cataacaatc ggcaacgtta cgaagctttg ggatttcggc
     5041 ggcaataatc agcgtctgcc ctccgatgat gaaatcaaga cagcgctcgg cagtgtcggc
     5101 tacaaaaatg tttcaatctc gggcaacgca gttcggataa ataaaggtca gtcgctcgac
     5161 ctcggcgcag tcggcaaggg cttcgcttgc gacaagataa aggatctgct tgccaagggc
     5221 agaacaaaat cagcagttgt ttcggtcggc ggcagtctgc ttatctacgg caacagaaca
     5281 ttccccatcg gcatagtcaa tcccgataac gacaagcagt caatgggaac cctgaagctc
     5341 aaggacacct gcgtttcaac ctcaggcaat tatgagaaat actttgagaa ggacggcaag
     5401 cgatatcatc acattctcaa cgcaacgacg ggctaccctg cgacaagcga attcaagagc
     5461 gttaccgtag tttgcagctc gggacttctc agcgacgctc tttcgacggt atgctatatt
     5521 gcaggatatc gcaaaagcgt cgatatcctc aaggaatacg atgccgaggc ggtttttata
     5581 ttcaacaaca atgcggtcaa ggtcaccgac ggacttgcgg acaagttcac ggtaaccgac
     5641 gacagctaca ctcttgacaa atgaagctat ttaaacgaac ggatttaatt ataatacttg
     5701 ttgtgcttct ttcggcggcg gcgatatcca ttccgagatt tttgaactcg gacaaattga
     5761 ccgcagatat ttacgtcgga ggcaagcttg aagaaacgat cgaccttttg gctgttgaga
     5821 aagaatacac actctctccg aacagcgacc cgaaagttga gataaccgtc ggcaagggcg
     5881 aaatatattt ttcacatgcc gaatgtaagg acaagctttg cgtcaaaagc ggaaaactga
     5941 cctccggcgg tgaaacggca gcatgtcttc cggcgagggt cgttatctcg gtcaaatcga
     6001 acaaaaacaa aacagatata atgacttatt gatatgaaaa acaaaaatct gacaagaaaa
     6061 atagccgtga cgggcatgat gtgtgcgctt gcgcttgcac tctcctacct tgaaacgctt
     6121 atccccgcct atccgggatt tcctcccgga gcgaagcccg gtctttccaa cattgtaacg
     6181 atgttcatgg caagctgtgc agggtcggga tacgcatttt tcataacgat aatcaaggga
     6241 cttttcgcag acgtcacaag aggaatgacg gcgatgctga tgagcctttc gggcggaatt
     6301 ctcagcactg cggcggcgtg cctccttttc ggattttgca agaacaaatt aggatatatc
     6361 ggcatcggaa tcatctgtgc cgtttgtcac aatatcggcc agcttgcggc ggcatgtatc
     6421 atttccggta cggcttcgct catcgtaggc tacggaccgc tgttgttaat attcgcgatc
     6481 gtgacgggct ttcttaccgg aacgattctc aaggcggtta tgcctccgtt ggagaaaatc
     6541 gcaaaattta aaaattaaat gggaaattcc catacagcaa aggggttgaa caaatgggta
     6601 aaattcacgt ggacggagtt atctcggcag tcaaaaaggt tcgcggcggt gtcaaggttg
     6661 accaccacaa gaacacggca ggctgtgagg tagttcgtat tcccgtcccg aagcaggtag
     6721 ttttgcctat gcagcagcac atcggcgctc cgtgtactcc gactgtcaag gtcggcgata
     6781 ccgttgcggt cggtcaggtg atcggagaca gcgataaatt tgtcagtgca ccgattcacg
     6841 catctatttc aggtactgta actgcaatcg gaaacgttaa gcttccgaac ggaaccatgg
     6901 ttcagggagt tactattgaa tcggacggtg agatgagact ttacgaaggg ctcaaggctc
     6961 ccgaggtaaa cacaaaggag gagctttgca aggcgatcag ggattcgggt cttgtcggtc
     7021 tcggcggtgc aggcttcccg actcatgtta agctcaatgt tccggcagat aagaatattg
     7081 atactcttat catcaatgcc gcagaatgtg agccgtacat aacggttgac taccgtgaat
     7141 gtattgacaa ttcatgggat atctgttccg gtatcaagac cgttaaagag cttctcaaca
     7201 ttccgaatgt cgttatagcg gtcgaggata acaaacccga cgctttcgag attcttcatc
     7261 agatcgttga taaagactcg ggatcggaca gctcaatcaa aatcatgcag ctcaaatccg
     7321 tttatcctca gggcgcagaa aaaatgatgg ttcagtcggc aaccggccgt caggtacctc
     7381 ccggaaagct tccggcagac gtcggctgta tcgttatgaa cgtcacttca atcgcgttcg
     7441 tcgcccgtta tctcaaaaca ggcaagccgc ttgtcagccg ttcaatgacc gttgacggca
     7501 tggctatcgc cgaaccgaag aatgttcgtg ttcctatcgg cacaagcttt gccgatgtcg
     7561 ttgagttctg cggcggattc aaagaggatc ccgttaaaat tattgcaggc ggtccgatga
     7621 tgggcatggc aatcaccgat gtaaattacc cgatttccaa gagtaacaat gctctgcttg
     7681 ctttctcggc taaggacgca aagattttca gagaaacgga ctgtatccgc tgcggccgct
     7741 gtgcagccgc ctgtccgatg agtcttgtcc cgaccaagat tgtccgtgac tcaaaggcta
     7801 aggacgttga cgctctgacc agggacggcg ttatggtctg catggaatgt ggaagctgtg
     7861 catattcatg ccctgcaaag aagccgctcg tccagcatat gagacttgca aaatcaatca
     7921 tcagagaggc aggtgcaaag aaatgacatt aaaaaacaag ctcgttgtca gcgcatctcc
     7981 tcacgaccgc agtccgagga ctgtaagaag tctgatgctt gacgttatta tcgcacttat
     8041 tccggcgctt atcgcttcgg ttatcttcta cggcccgaga gcgctgctca ttgaatgtgt
     8101 cagcgttgcg acctgcgtaa tatttgaata catcaccaga atcgtattca agcgcagtca
     8161 gtctatcggt gacctcagcg cagttatcac aggacttttg cttgctttca accttccggt
     8221 ttccattccg ctttggatgg tagttatcgg ctcgctcgtt gcgatagttg ttgcaaagca
     8281 gttcttcggc ggcatcggtc agaactttgt caatcccgct cttgccggac gtattgttct
     8341 tctgacctcg ttccctgttg cgatgtcaac atggactcag ccgctcacat ggactatgtc
     8401 aaagcttgac ggcatcacct ccgcttctcc cctctcgtct attcacgaga tgacttcggg
     8461 cggtgcggct cccgacgttt cactcgttga aatgctcatc ggctggcgag ccggctgtct
     8521 cggtgaaacc tgcgccgttg cgcttctcct cggcggcatc tatcttgttg tccgtaaggt
     8581 catatcgccg acaatccctc ttacatatat cggcacggtt gccgttatta tgctcatcgc
     8641 gggcaagggt gatttccgct ttgttgcata tgagcttctc ggcggcggac ttatgctcgg
     8701 cgcaatcttc atggcaacgg attacacaac ctcaccgatt acatttaagg gcaagataat
     8761 cttcggtata ggttgcggcc ttattacttc aataatccga attttcggca atcttccgga
     8821 gggcgtttcc tattcgataa tcatcatgaa tattcttgtt ccgctgattg aaaacgttac
     8881 gcttcccaag ccgttcggca ctccgaaaaa agaaaagaag gaggctgagg caaaatgaaa
     8941 aaacaaatca taattcctac ggttgcgctc tttattatct gccttgtcgc aacaaccttg
     9001 cttgcgctgg caaataacgt aaccgctccg ataatcgaaa aaaactcggc ggaatccgag
     9061 gctcagtccc gtatggaggt tcttgcggat gcaaagagct tcgaggacaa gacttttgac
     9121 ggcataagct acgcagtcgg tcttgacggc agtaagcaga cggtcggcat ggttttcaca
     9181 acaacgtcaa agagctacgg cggcgacctt attgttatga caggcgttga caatgaaggc
     9241 aaggttacgg gaattcaaat tctctcaatc agcgacactg caggtcttgg tatgaaagcg
     9301 cagacagatt ccttcaagtc tcaattcaag gggcttgttg acggagtcaa ggtagccaag
     9361 aacagcgccg atcacgacag caacgagatc ctcgctctca cgggcgcaac gatcacgtcc
     9421 aacgccgtaa ctacggcggt caacgaggct ctcgcaaact acaaaactat cacagaaaac
     9481 ggaggtgcta actgatggct gaaaagaaat ccttaggcaa ggagcttacc aagggtttta
     9541 tcattgaaaa ccctgttctt cgccttgtac tcggcacctg ccccaccctc gccacaacga
     9601 cctctgtttc aagtgcgatc ggcatgggcg tttcggcttc aatcgttctc atctgctcga
     9661 acatcgttat ttcggcattg agaaaggtta ttccgcagaa ggtccgtatc ccggcatata
     9721 ttgttatcat cgcttcgttc gttacaatcg ttcagatgct tgtcaaggca tttgttcccg
     9781 tgctcaacga acagctcggc gtttatcttc cgcttattgt tgttaactgt attatcctcg
     9841 gcagagcgga agccttcgca agcaaaaacg gtgtgctcgc ctcggcagtc gacggtctcg
     9901 gcatgggaat cggcttcacc ggtgcactct ttgtcatggg caccatccgt gagattctcg
     9961 gtgccggcac attcctcaac ggaatcagcg acctcatcgg cggcagcttc aacggtttct
    10021 caatcccctt cctaaacgaa aacccgatgc ttatatttat tctcgctccc ggcggattct
    10081 ttgtattcgg catggtaatg gctcttgcaa ataaaattgc cgagtcaaag ggcaaaccca
    10141 aggctgtact ccacggctgt gaaaactgcc cgatggctaa atcctgctcc atcagagaca
    10201 ctcacgagag ttgtgaagag aagaaggagg ctgaagcata atggaaatga caaaaggcct
    10261 tttgagcata ttgctcacaa tgattctcac tcaaaacttc gttctttcac gatttctcgg
    10321 catctgcccg tttctcggcg tttcaaagaa gctgaatacg gcggtcggca tgtcacttgc
    10381 cgttatcttc gttatggctc tttcgacggc ggttacatat cctataaaca agtatctgct
    10441 tgtcgccaat aatcttgaat atcttcagac gatcgttttc attctcgtca tcgccgcact
    10501 cgttcagatg gttgagatta ttctcaagaa gtatatgccg gctctttaca atgcgctcgg
    10561 tatctatctt ccgcttatca caacgaactg cgcggttctc ggtgttgtaa cactcaacgt
    10621 tgacaacggc tacaacttta tgcagtcgct catctgtgct atcggcggcg gtgtcggatt
    10681 tatgattgcc atgatcatgt ttgcaggtgt tcgttcaaga cttgaatcct gcgacattcc
    10741 gaaattcctt aaaggtctgc ccatcacact tgttgccgct tcgcttgttt ccctttcgtt
    10801 cttgggcttc gcaggcgttg ttgacaagat ctttgcataa ggaggcgcaa atatgaatcc
    10861 tattgtttta gcaattctga tcgtttcggt catcggactc attgctggtc tcggacttgc
    10921 agttgcctcc atcgttatgg ctgttcccgt cgataagaag gcggaggcta tccgtgaatg
    10981 tctccccggt gcaaactgcg gcgcatgcgg cttctccggt tgtgacggct atgcggcggc
    11041 tctttcaaag ggtattacaa caaacacagc tctc
//
LOCUS       DAWBSW010000054        10968 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1280_length_10968_cov_2.970494, whole genome shotgun sequence.
ACCESSION   DAWBSW010000054 DAWBSW010000000
VERSION     DAWBSW010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1280_length_10968_cov_2.970494"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1681..1704
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8440..8449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3011 a         2496 c         2513 g         2914 t
ORIGIN      
        1 ataatgatgt tgtcttcacc gacagcctcg tgaatttcaa catttgcacc gtccattgtg
       61 ccgagtgtga cagcgccgtt tatcataagc ttcatgttgc cggtgccgct cgcttccgtt
      121 cccgaaaggg agatctgttc gctgatatcg gcggccggca taaggtgctc tgccattgta
      181 acattataat cctcaagata tacgaccttg agacgtcctc tcacgtcggg gtcgttattg
      241 ataagctcgc tgagtgagca gatgaagctg ataattttct ttgccataaa gtagccggga
      301 gcagctttag cggcaaaaat ataggtgtgc ggagtgaagt ctccgtcggg attttccttg
      361 attgcaaggt actttgaaag aatatggaaa gcattgaggt gctgacgttt gtattcgtgc
      421 agtcttttaa cctgaacgtc aaagattgat gtcggatcaa gctctacccc gtttgatttt
      481 ttaacaagct cggtaagtct ttccttgttg tgaagcttga ttttttcaag cttcttgaga
      541 acctcggcat cggttgcata gggtttgagc ttttcaagat catcgccgtt atagacaaat
      601 ccgttgccga taagctcggt aagaagcttt gtaagctcag ggttggactg gcagagccaa
      661 cggcggtgag caataccgtt ggtaacgttt gtgaacttgt cgggagtgag cgaatagaaa
      721 tccttgaaaa cactctcctt gagaatctcc gaatggagag ccgaaacgcc gtttacgcag
      781 tgtgaacccg caacggacat atttgccatt ctgataaccc cgtttgaaac gattgccatg
      841 cgttcaacct tgtctgcgtc accggttgca ttccagacat agctgcgaaa acggttgtca
      901 atctcttttg taatctcgta tattctcggg agaagtcgtt tgaaaagatc ctcgggccag
      961 cactcaagag cctccgccat aacggtgtgg ttggtgtaag cgacggtatc ggtgacaatc
     1021 ttccacgcct catcccagcc gtagccgcag tcgtcgagaa gaattctcat cagctcggga
     1081 atggcaaggg tcgggtgggt atcattgatg tgaattgcaa ttttgtcggc aagattttca
     1141 accgtgccgt aaactctcag gtgattattg ataatatcct gaattgaagc agagacaagg
     1201 aaatactgct ggcgcagacg aaggctctta ccctccggat gattatcgga tggatagaga
     1261 accttgctga tgacctcagc cattgcgttc tgctccattg cgcgcatata atcgccctgg
     1321 ttgaacatat ccatgtcaag tcccggagcc tttgcgctcc aaagacggag aacgctgatt
     1381 cccttaccgt ccttacccga aacatacata tcatacggaa tagcttttac cttattgcag
     1441 tctcgctgct caacatggtg gtactgattg tcccagctct cgtcaaccca gccctcaaag
     1501 aggatatcta ctgcctcctc ctcacgggga acaagccaaa catctccgcc cggcagccag
     1561 aaatccggca gctcggtctg ccaaccgtca acaagcttct gcttgaaaat gccgtattcg
     1621 taaagaatcg aatagccttt tgcgtaatag ccctgacttg caagaacgtc aaggtagcat
     1681 nnnnnnnnnn nnnnnnnnnn nnnngttctt gagtgaacgg cccatgagaa attccatgca
     1741 aagataataa atcttctttg aatcggattt ctttgcttca ttgtggaatt cctttcttcc
     1801 ctgcatcatc atgtcacgaa gaataagggc aattgccttg tagtaatgct cataggtagc
     1861 ctcttcgggc gaaacgccgt agaaatgcga aagcttatta acaataagct ccttggcttc
     1921 cttttttgta agtttataat tcataaaaag cctcctgact tgattacctg atttaatatt
     1981 tttattttat accatttttg tagaaaatac aacaccctat ttaaaattgc ttatacgaat
     2041 ttcaaccctg tattttatgc acttcaacga aaaatattta ttattttagc atataagaaa
     2101 aataattata aaatttaaac aaaataacgg cttaattacg ttataacagt aaatataaat
     2161 gctttattaa aacaaataat gtcaaaaaaa tacgaaaaaa ctctttatca aaattgaaaa
     2221 atatagtata atgattttat aatcttaata tttgcgatgt ttttcgcaat tattctgcgg
     2281 aggtacagtt atgggaaaaa acaaagtcag actcacgatc ggaggattgg attattatat
     2341 tacgaccgat gaggagatcg cctatatgaa aaatctcggc gaggaccttg acgacgcaat
     2401 cacaggtctt atacacgacc acccgagact ttctcaggtt caggcggcgg ttctcagcgc
     2461 ccttgactat gccgacaagc ttaaacaggc tgaaaaatca agcgattttt taaagtctca
     2521 aattcagatg tacatggagg atgccgcaaa ggcaaagacg gaagccgaaa tggctcgccg
     2581 tgaagccgag cgcatgacaa gagacctcag gagcatccgc cgcagtctcg aagaaaagga
     2641 aatgctatga taaaacctga aattcttgcg cctgccggtt cgccccaggc gcttattgca
     2701 gccgtgcgaa gcggagcaga tgccgtttac ctcggtatta aaaatctgaa cgcgcgccga
     2761 agtgcagaaa attttgacga tgagcagctt agagaagccg ttgcatattg tcacaagcac
     2821 aacgtgaaag tgcatttaac gcttaacact cttgtatccg acggcgagct tgaaaaagca
     2881 caggaagcgg ttcagcttgc atgtgaggca ggcgttgacg caattatcgt gcaggatata
     2941 ggaattgccg agcttattca cagaacggcg cccgatatgc cccttcatgc ttcaactcag
     3001 atgtccgtcc agaccgccgc agggcttaaa agacttaaaa gactcggctt cacccgtgcc
     3061 gttcttcccc gtgaattgtc aaaagaagaa atcaaaaatc tttgcgaaaa ttcgcctgtc
     3121 gagcttgaat gctttgtaca cggtgcgctt tgtatgtgcg tttcaggcca atgccttttc
     3181 agtgccgttc tcggttcacg aagcggcaac agaggcgcat gtgctcaacc gtgccggcta
     3241 cccttctcgg ttgaaaacgg aacaggccat gacctgagct taaaggatct ttcgctaatt
     3301 gattatatat ccgaaatggc tgaaatgggc gtttgctcat tcaaaataga aggtagaatg
     3361 aagcgtccgg aatacgttgc cgcagcggtc aaggcttgcc gcaacagcgt ggacggcgtg
     3421 acggataacg ctctccgaga cgatctgcgt tccgtgttct cccgttcggg tttcacggac
     3481 ggctattaca gaaacaagct cggatatgac atgttcggaa ttcgccgcaa ggatgatgtt
     3541 acggcggcat ccggcgttct taaaaagctt gaaaagctct acgaaaaaga gaaagcggat
     3601 actgttgtcg attttgcttt cacggctttg aagggtgaac cgatatcgct tgccgcaaga
     3661 gcgggagcaa aaaacgtttt tattgaagga gaggttcctc agctcgcaat gaataaggcg
     3721 acaactgccg aagcggttca taatcagctt tcaaaggtcg gcggaacaag atttttgtgc
     3781 ggagatattg atattgagct tgacgacgga ctttttattc ccgtctctga acttaacaag
     3841 ctccgacgtg aggttatcga aaagcttgac gggaacaaag ctcagtcaaa gagcttcacc
     3901 cccgaatata ttaaaattct gccgcacaca ccgggcaaaa aacggcttat ttgccgcttt
     3961 gccgacattg acagtatacc cgaaaattgg gattatatcg aacaggtcat cgtgccgctg
     4021 ggagacgagc aaaatgttaa aaaatcgctc agagtcagcg tcgaagtgcc gagagggatt
     4081 ttttcaaatg atgaaaagat tctcgaaaaa ctgaaaagcg caaaggaata cggcataacc
     4141 gaagcatggg caggcacact tgacggaatt gtgcttattc aaaaggcagg gcttaaaccg
     4201 aatgcctttg tcggctcaaa cgtgttcaac tcgctctccg taaagtcgct cgaaggtatg
     4261 ggaataaacc gaattctgct ttcgcccgag ctaaccttgg ctcaggctca ggcaatcggc
     4321 ggagatgcgc cgaggggcgt ttttgcttac ggcagactgc cgttgatgct gacaagaaac
     4381 tgtccgcaga aaaacggaaa atcgtgtgca gaatgtaagc gcacgggcaa gctgaccgac
     4441 cgcagaggaa ttgaatttcc gatcgactgc cgctcaggct gcagcgaaat tctcaacagc
     4501 gtccctgtct atatggcgga caggctcagc gagataagaa acatggactt cattcttctc
     4561 tattttacaa acgagagcaa ggaaaaaacg tcgctcgtga ttcaggatta tattaaaggc
     4621 tccaagaagc cgcacggcga atttacaaga ggcctccttt acaggggtgt tgaataaaca
     4681 aaatataagc aacaacaaag ccgcaggaat caccctgcgg catttcttct tatttcttct
     4741 tatttcttct ttttcttcat aaacgacagc ttcgggagct tgattttagg ctcggaggaa
     4801 acttcatctt caaacgcatc gctgttgttc caaaggtgga taattccctc atcgttgaga
     4861 agcttgacga tgattataag aatcggcaca aggaagattc cgataaatcc gaacagttgt
     4921 gaaccgatat acatacccat aagggtgacg aacgacggca aatcaaccgt tccgccgacg
     4981 attttcggct ctattatctg tcttacaaca tagataattg catagacaac aaacagtcct
     5041 attgctaatc cgaccttgcc ggttatcaga ctgtaaagcg cccacggaac cataaatgta
     5101 cccgttccga tgacaggtag gatatccagc acggcgataa tcagcgacag tgcaacaatg
     5161 ttgccgtcct tgtaaattcc gataagtgta aggatattca atccgacaaa aatctcaata
     5221 cccgttatgc aaataataag aacatatgat tttataagtt ttccgagtga tttaaataca
     5281 attcgcttgg ttgcggaaag cttaccttca ttttcctccg aaatctgacc cttgataaat
     5341 ataacaagcc ggtcatagtc aactgccata aagcaagagg aaactatcgc tatcataacg
     5401 gcaacaaagg cggacggtat ctgctttgct gtgctccaga ttccgctgag cggagttgaa
     5461 aggctcgcaa ttgagaactt ctcaccgttt gacgccttgt ttacgataaa gcttgcaatt
     5521 tcgctggcac tcttttcctt gagagcgtca aagaacggta tcatcgactg tgttgctttt
     5581 gtttcaagac catcgggtaa aaatttgata gcattaagcg accaattctt gatattctca
     5641 ataagcgtcg gtatatccgt cagctttgac tgaataaaag caataaggct ttttattccg
     5701 tcgaccagct tcactccgat aagcgagatc aaggcagcaa ttaccagata caaaagaaga
     5761 acaagaatcg ttgcggttat accctttttg atttttgtct ttctcgttat aaaattggtc
     5821 ggcttttgca ctattaccgc aataaaaaaa gcaataagaa acggtgcaaa catcgagaag
     5881 cagtatttca aaaataaata tgccaagcct aatacaatgg cgtaatagac tatatttata
     5941 atttttttac gtcttacttc ggctttatcc atacgcattt tcactccata gctgttaact
     6001 ttattcctta caatgaatat attataccat aaaagagaat aatgcaaata aaaaatgaaa
     6061 aaatttcaaa aaacacttta ttattttaca cttttatgat aaactaagtt gattatatcg
     6121 tttaaacgta tcagggagtt gaaaacaatg gacatagctg ttttaggcta cggtgttgta
     6181 ggttcgggtg ttgcggaagt tatttcgctc aacgagcaaa aaattacagg caaaatcgga
     6241 gaaaaaatga acgtcaagta tatccttgac ctcagaaaat ttccgggtga caaaaacgaa
     6301 gataagataa tagatgattt caatattatt cttaacgacg gcgatgttaa aattgtcgca
     6361 gaggcaatgg gcgggcttca tcccgctttt gaatttgttt cctcctgctt aaaggcaggc
     6421 aaatccgttg tcacttcaaa caaggagctt gtcgccgaaa aaggtgatat cctgcttgaa
     6481 ctggcaaagg aaaacaatgt taactttatg tttgaggcac gcgtcggcgg cggaattccg
     6541 gttctgcgcc cgattattca gtgccttgcc gcaaatgaaa taaccgaggt tgccggcata
     6601 ctcaacggta cgacaaactt cattctttca aaaatgattt ttgactcaat gagctttgat
     6661 gatgctctca agctggctca ggacaacggc tatgccgaaa aagacccgac ggcagatatc
     6721 gagggctttg atgcatgccg caagatttgt attcttgccg cgctttgctt cggcaatcat
     6781 gtttatccca agtcggtcta taccgaaggt ataacaaaga ttacgcttga agatgttgat
     6841 tatgcacgct catggggcgg cgtaatcaag cttctcggca gagccaaaaa ggtagacggc
     6901 aaaacctccg caatggtttc gcccgcattc gtttcaaatt caagtattct ttcgggtgtc
     6961 aacggagtgt tcaattccgt tcttgtccgc ggtaatgcgg tcggcgacgt tgctttctac
     7021 ggtcagggtg caggcaaaat ggcaacggca agtgcggttg tcggcgatat gattgactgt
     7081 gcacagcaca tcggacgcaa caagggcata agctgggata aggcagttga cggttctctt
     7141 atcgatcata acgaggttaa aactgctctt tacgtccgtg caacaggctg caaggaatgt
     7201 gcgctcagca aagccggtga gcttttcggc agcatcagcg ttctttcgag agagaacgct
     7261 ccggaggatg aatttgcttt cgtcacatcg gaggacaggg agggcacgct cagagccaag
     7321 ctcggtgaga taaagaacat caatgtgctt tcaacaatca gggtagttaa ttattaaaat
     7381 ataaagtggt gaattgatca tgggacttat agttcagaaa ttcggcggca gctcggtcgc
     7441 caacacagag cgtgtcatga acgttgcaaa aattgtaacc gacacctaca aaaaaggcaa
     7501 tgacgttgta gttgttgttt ctgctcaggg tgacacaaca gacgacctta tcgcaaaggc
     7561 aagagagatc agcgctcata attcaaagcg tgagatggat atgctcatgg cagcaggcga
     7621 gcagatttcg attgctctgc ttgcaatggc cattgacgag ctcggctgtc cggcgatttc
     7681 gcttctcggc tggcaggcag gattccgcac agattccgtt tactctgctg caagaatcaa
     7741 aaaggttgag acagacagaa tcagaaccga gcttgacaac cgcaagatcg ttgttgttgc
     7801 aggcttccag ggtcttaaca agtatcagga catgacgacg ctcggccgcg gcggttctga
     7861 cacaagcgca gttgcaattg cagcggctct caacgccgac agatgtcaaa tttttaccga
     7921 cgttgacggt gtttacaccg ccgatccccg taaggtagcc ggcgcacgca gactccagga
     7981 aatcacatat gatgagatgc ttgagcttgc aacgctcggt gcacaggttc tcaacaaccg
     8041 ttcggtcgaa atggcaaaga aatataatgt tgagcttgag gttatttcaa gccttaaccc
     8101 gattccggga acaatcgtaa aggagaccac taaaatggaa aagatgttag tcagaggcgt
     8161 aacaaaggac aaggacgtat caaaaatttc tatcgtaggc gttgaggatg ttccgggaat
     8221 cgcattcaag gttttctcac agcttgctaa aaacaacata aatgttgata tcattctcca
     8281 gtctgtcggc agagataata caaaagatat cgttttcact gtcagcatgg caaatgaaga
     8341 tgaagcagtt gaatgtatca aggctctccc gttcatcggc gacgcaaagg tattcagcga
     8401 taacacggtt gcaaaggttt ccgttgtcgg cgccggtatn nnnnnnnnnc cggtatggag
     8461 actcacccgg gcacagcttc aaaaatgttt gaggctctct acggcaaggg aatcaacatt
     8521 cagacgattt caacaagcga gatcaaaatc tccgtactta tcgacgagaa ggatgcagac
     8581 aaggctgttt ccgcagttca cgaagccttc ttcggcaatg actgagataa ataaagcttt
     8641 aatgcacggt acgatattcg caccgtgcat ttttgttttg taagcaaatc gcctttatat
     8701 attcggttga attagctgtt tttgcaaatc tatatagttg aatcgaccgt tccgcctaat
     8761 gccattgaca aacttcatat attatcttaa tattacttta cggctccgat tgctgcgaca
     8821 caaaaccacc gccgagcgga aatctgctcg gcggtggtgc cgtttatata ttcagctttt
     8881 tatcaataca ttccgccgcc ctggcttgcc attgctgcgg cagcagctgc gtcagcctgc
     8941 ggatcgggct tgtcggcaac gagtgactcg gttgtgagta ccattgcggc aacgcttgat
     9001 gcgttctgga tagccgaacg agtaaccttt gtcgggtcaa gaatacctgc ctcgaacata
     9061 tcaacaaact cgcctgttgc gaagttgtag ccgaagcctg tcttgccgct cgaaacgata
     9121 tcgtttacga taacagagcc ttcaagacct gcgtttgcgg cgatctgacg tgccggagct
     9181 tcaagagcct tgagaacgat cttgatacct gtcttttcgt cagcgtcctc tgttgaatca
     9241 aggagggcct taactgccgg gatagcgttg agaagtgcaa caccgccgcc tgcaactgta
     9301 ccctcttcaa cggctgcctt tgtagccgaa agagcgtcct cgatacggag cttcttatcc
     9361 ttcatttcgg tctctgtagc agcacctaca cggataacag ctacgccgcc ggaaagcttt
     9421 gcaagtcttt cctggagctt ttcacggtcg aaatcggagg ttgtgttctc aagctgtgac
     9481 ttgatctgag caactctgcc cttaatctcg tccttatcgc ctgcgccgtc aacgatgatg
     9541 gtgttctcct tggaaatctt aacctgacgg gcctgaccga gctgagccat tgttgcgtct
     9601 gcaagctcaa gtccgaggtc tgatgtgata acctcgccgc ctgtgaggat agcgatatca
     9661 cggagcattt ccttacgtct gtcgccgaag cccggagcct tgacagcaac acaggtgaat
     9721 gtgccgcgga gcttgttgac aaggattgtt gaaagagcct cgccctcaac gtcctcagcg
     9781 atgataacaa gcttcttgcc tcccttgaga acctgctcaa ggagcggaag aatttcctga
     9841 atgtttgaaa tcttcttatc tgtaataaga atgagagcat cgtcgataac agcttccatc
     9901 ttatctgtat cggttaccat gtagggtgaa atgtaaccgc ggtcgaactg cataccttca
     9961 acaatgtcgg aatcagtaac ggctgtcttt gattcctcga cggtgataac gccgtctgca
    10021 gtaacctttt ccatagcctc ggcaatgatc ttgccgattt cttcgtctgc ggcagaaacc
    10081 gtagcgacac gggcaatatc atttgaagat gtgacgggct ttgaatttgc cttgacagcc
    10141 tcaacggctg cgtcaacacc catcttgatg cccttcttga ctaccatcgg gtttgcgcct
    10201 gcggcaacat tcttcattcc ctcacggaca aaagcctgtg caaggagtgt agcggtagtt
    10261 gtaccgtcgc cggcaacgtc gtttgttttt gttgcaactt ccttaacgag ctgtgcgccc
    10321 atgttctcga acggatcctc aagctcaacg tcctttgcga tcgttacacc gtcgttcgta
    10381 atcaacggtg agccgtactt cttgtcaaga acaacgtttc tgcccttggg gccgagtgtg
    10441 attttaactg tatcggcaag cttatcaata cctgcctgga gagcctttct ggcttcttct
    10501 ccgtaaataa tctgtttagc catgatgaat tcctcctgtt attctactat tgcaagaata
    10561 tcgctctgac gaacgatgat gtactcttct ttgtcgagct taacttcggt gcctgcatat
    10621 tttccggtga ttaccttatc gccgacctta acatacattt caacctcttt gccgtcaaca
    10681 agacctccgg gacctacagc aaggacctca gctatctgcg gcttctcctt agcggaagat
    10741 gcaagaataa ttccactttg tgttttttct tcagcctctg tcatcttaac aacaacacgg
    10801 tctgcaagcg gtttgatttt cattgtgaaa gcctcctata aaaactaaat atttgtcgtt
    10861 ttagcactct tgttccctga gtgctaaata tattatagcc cgtttgccgt aaaaatcaag
    10921 tgtttttgaa aaaattaaca atttgataat attttctgca aataacag
//
LOCUS       DAWBSW010000055        10853 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1298_length_10853_cov_2.622615, whole genome shotgun sequence.
ACCESSION   DAWBSW010000055 DAWBSW010000000
VERSION     DAWBSW010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_10853_cov_2.622615"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    694..703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3010..3019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7121..7130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2905 a         2393 c         2083 g         3442 t
ORIGIN      
        1 ttcttgcaac atttaagagc tggcttctct tttgcttacg catatttttt cttcctttca
       61 aagaaattgc tttttgtcac agtcagggga aaaagttgtg tgtggcggca gcttaagcca
      121 ccacactttt tatcccctgt gaccgtaggg aaaaatcact tttaatatat ttatatatat
      181 tgtttttcct tccctacgga aattttcgat aaataatcga ctttttccct cactcggaaa
      241 ttttcgataa actaatcgaa tttttccttt ttcaggtttt aagggaaaat cgggaaattt
      301 atttttttct atcgactttt tccctcactc gattttaccg acaacaccgt ctttaatcca
      361 aaaatctgag tgttcctcaa cataatttct gacagttttt tcactctttc cgatatattc
      421 ggataagtct ttaagtgatg atttaccgtc attattacaa tactcaaaag ctccttcaag
      481 agagacttgc cgatcctttt tattctcctg cttagatttc cttttgttaa aattcttctg
      541 ccaaggtgag cctttagaat tctgagtatc aatatctttg aggatatccg catcgtctat
      601 gacatgtacc ggatacttaa accaaagatt gataggattg aatttcggat attctctgag
      661 cgttccttca atcctccacg ccgtaaagct ttcnnnnnnn nnnctgttaa tatctttcat
      721 cataagttta aagcttgtag gctttaaaag ccccctggcg gcttctgtcg ctttgtcagc
      781 gacaagtaaa tcatcaatgg caacttcgtc tttcatatta aagcgtgaca gccaatctga
      841 cgcaacttca catactgcct tgttgtctaa ctgtttcttt attgcttcgt ccaaatcaag
      901 ctctatgagg tcaatcagcg catcggggtc acgagcaaaa actcccgagc ctgatgctct
      961 gtccattgac tttttctcgc cctgcaaacc ttttgagtga tggtgacaat aaataacggc
     1021 acagccgagt tcggtacaaa ccttatcgaa ctgattacag aattttgaca tctgttcggc
     1081 tgagttttcg tcacctgtaa tgatcttata tatcggatca ataatgattg caatgtaatt
     1141 ctttttagct gctcggcgaa tcattttcgg ggcgagctta tccatcggaa tcgacttacc
     1201 tctaaggttc caaatatcaa tgttactgat attttcaggc tcccaataaa gtgaattata
     1261 tacatcttta aagcggtgca aacatgaggc tctgtcaagc tcaaggttta catacataac
     1321 cttaccctgc gtacaatcaa aaccaagcca tttcttaccc tctgcaatgg cacagcacag
     1381 ttcaatcaaa gcgaatgatt ttcctgcctt tgacggaccc gcaacaagca ttttatgccc
     1441 ctgccgaaga ataccgtcaa taagcggcgg tgcaagctcg gggaggttat cccatacact
     1501 ttcaagcttc tcaggatcgg gaagatcgtc gtcaacgccc tcaatccatt cctgccattc
     1561 agcccagctt tgtttaccga tattggtttc aagtaaatac tgtttttgac ctttgcgtgt
     1621 cactcccggc atacgggaaa gtcttgaagg attcttattc tgcgaatcaa tcgtcaatcc
     1681 gttcttttgg catttagcat aaagatagtc aactcttttt cgatactcgg aataatccgc
     1741 agcgtcaatt ttgacaatgg catgtaagct ttttccgccg ctgtaaacaa gtgcggcaat
     1801 aggcaattca agctcgtgta aaagggcgtt ttgcatttca agctccatgt tatccgattc
     1861 aacaagcgca aaacgaaaat ctgttacatt gtcgtttttt acaccctctc cgtcgagcgg
     1921 attaaacctg atccacgccc ccgcttcttc tctgtaatct ccgagaacag agccgatatc
     1981 tcccttgcag ttgtttaatg cctgaataag ttctcctgcc gttcgatcat aatttccttt
     2041 tgtaggaaga tatttgccgt ctttttccca tgtggtagtt acgtatccga cattttctgt
     2101 actgtcaaat aatgtttcaa gatatgtaat aagctgctta acaggattcc aatgttccgg
     2161 aatgttcagt tcacggcttt cgacccatga tttatcgatt actcgatcct tgcctatttc
     2221 agaatcccaa tcaagctccg agccctcaaa ggacggcttc cagccttgat cttttgcaat
     2281 ttgaaaaata gtaccggctg taacgggatt ttgatttcct tggaaggttt tccattttct
     2341 ttctcattcg ccgttatgat atctgcaatc gtttttactc cattcatccc atacactgac
     2401 ggtatagccc tcttctttga gagccatacc gacatttacc cattcctgat acgaacataa
     2461 cgcagggtta atgtgttcaa gtaattcaat tattccgatt tcaccatcct gcataggtca
     2521 ttgcctcctt atgttcaggt gtatatttgt ttggtactat atcgtgcggt actcgccaac
     2581 tgttggcagc aatgcgatca attagttttc ttgcctcttc aaaacgccat gttccgacat
     2641 gctgaaagcc tctaccctca aggaagcgaa tctgtttcgg cgtagtcaag ccctcgtctc
     2701 ggcgtttgtt taaacggtca agaagcattt tagccttgcc tgcatttcct acttcatcgg
     2761 ggaaaatacc gaatttttca agtgcggcaa gctgtttatc tgacggtgga gacatctccc
     2821 agccaaacga cggcacataa cctgaaagat cttcggcttg tatagacatc tcaaattgaa
     2881 gtggatcaac aagtttacgc ttgcgatttt tcatctcagc aagagctttc gccaatgctt
     2941 cttctctctg agcaacgacg tcggtttctg cctgctgttc tgcttcttca atatctatcg
     3001 ggcaagctgn nnnnnnnnnt ctttcatatt ttcaatcatt ttatcggcaa cttcgggatt
     3061 agtgcaaata aggtgtgccg gtctgcaaag ctcatgcttc tcggtcatcc aaaggaaatc
     3121 taacaaaaga agttccttct tgccgggaga aagccttgtc cctctgccaa ccatctgact
     3181 gtaaaggctg cgaacctttg tcggtctgag gactatgaca caatcaacgg acgggcagtc
     3241 ccaaccctcg gtaagaagca ttgaattaca aagaacattg tacttatcgt tctcaaagtc
     3301 ttctaagacc tccgccctgt cctcacttgt tccgtttact tcggcagctt taaatccttt
     3361 agaattcaaa atatccttaa acttctgact cgttttaata agcggtaaaa agacaactgt
     3421 ttttctgtcc gagcaatgct gcaacatttc atcggctatt tgctcaagat acgggtcaag
     3481 tgccgtatca agatcaccga ccttaaaatc tccgctctgc atgccgactt ttgtaaggtc
     3541 aagcttaagc ggaatagtca aagcttttat cgggcagaga aaaccgtctc tgattgcctg
     3601 cggcagctta tattcatatg ccaaactttg aaagtattca ccgagatttt tcatgtctcc
     3661 tctgtctggt gtagccgtta cgcctaaaac atttgcatta tcaaaatgct gtaatacctt
     3721 ttgatagccg tcagaaatgc aatgatgagc ttcatcaatg attattgaat taaagtagtt
     3781 ttcatcaaaa cggttaagtc gcttttctcg ctgtaaggat tgaaccgaac cgacggcaat
     3841 tctgtaccaa cttccaagac agctttgctc ggctttttct gttgcgcaaa caagccctgt
     3901 tgcctttctg attttgtccg cagcctgttc aagtaattcg cctcggtgag caagaattaa
     3961 cactcgctcg ccgtttttta cacattcttc ggcgattttt gcaaacacta tcgttttgcc
     4021 tgtacctgtc ggaagaacaa gaagtgtacg tttgttgccg gtatgccatt catgaaaaac
     4081 agaatccttt gccgcctgct gatatgggcg cagttccatt accaaccacc tttatttcct
     4141 gtcttccacg acggtgtagc gggtgcagac tgctgagcag gttcaaggaa gcgtttaatc
     4201 ttgtttgact gccccgttgt accatccttt cgtgtaaatg tatcaatata taccttacat
     4261 cttcctgtct tgccctgtat gtcattccaa ttcatggcat atttttcgcc gtgcttttta
     4321 agcccgatcg caataaaaaa ctcactgagc tttccctcac accttgtatg gagaaaaaga
     4381 taatgcagaa tagtggtact tcccaattct ccgccgtcaa tttttatttt tacgataacc
     4441 tcattacaag acggcagctt tgcaccgccc tgatatcttg ctctttcaaa cgatacaacg
     4501 gtaaaattgt aatcgccttc gggaaataat gtaaattcgg attccatgtt tatttcggaa
     4561 ttccaatcaa gctctctttc aatattgtta ttatccatga taaatctcct ctttaataat
     4621 taaatggtaa gtcgttgttt tccttaatgc tcgcaagtac cttatcccat atgctgacga
     4681 catattcaac aaagtcggca gggtaatttt ctataggtgt tgattcgggg aaatagcctt
     4741 gtaaagctat ggtttcacgg agcatttttt ctgtaactcc gctcgcattc atcaaatccg
     4801 ccaaaggctt tgctatttta ggattgattg tttcatttgc aggttttacc ttctgctgag
     4861 tttcaattgg tttatctgtc tcagctgtgt tatcgataat ttcttcaaaa tcgcctctgt
     4921 caacagttgt tgtcggctga ggtacagact tcttgaagtc aggaataaca tgagctatcg
     4981 acgaaaaatc gaaaggtatt tcctccggca atccgtcacg gttcttggca tcccaacaag
     5041 gattatgagt ggtgtacatc actcgctgac cgccctgtgc tttatacttc tgtgtttttt
     5101 cattaaacac ggtaatggtt ttgtaatttg caaaaagcac catatccgcc cactctttaa
     5161 taagcggtga gatctgagag cttgtctttt taccgagctt aagttcccaa cggtcatatg
     5221 cgcccatttc gtcaggctgc tcaaacttac gaagctgagc gtgcgccgta agaacaacgt
     5281 taatattctt tgcgattact tcatcaagaa gattaaggaa acgcccgatt tcttcttttt
     5341 catatacata accgtttccg tagccaaaat cttcaatacc ctttttgttt gcccttgcac
     5401 agatatcgtc aatacaaagc tgttcagccc aatcaatggt atcgacaacg agtgtgctgc
     5461 ataattcggg atgatcaatg atatatttga tctgttcctt aagcatggtc cagcttgtag
     5521 gcttgtccat acgcttcacg ttgagttttt ttgtgcttcc ctcggtgtca ataaaaatgg
     5581 gatcagggaa acatgaagca aaagttgatt taccgattcc ctctgaaccg tataacacga
     5641 ctttctgtgc ggaaacaatt actcctgttg atatgttcat gaattccact ccttatattt
     5701 tgctttttct tccggcttaa cataaccgtc ctcgataata atggaacatt cgttacctgt
     5761 gcttactctt gttgcgattg cctgtaagcc ctcggattca agccacttgc cgaaattctg
     5821 caaggtttct tcgtccatct gttcaagctt atcaagcaaa acaaatccgc aattcggatt
     5881 aagcttgcga acaatggcag ttgttacctt aagctgatcc gatccgctga ggttatccca
     5941 tttaaagccg ttatatatca gctctccgtt ttccaccgaa agccccggaa gaggaagctc
     6001 cgcactactg agaagatcgt acttttcttt gcgtacatca tcgatttcac gagataacgt
     6061 atcgtactga tctgtatatt gttttgcttc ttcttcaatg tgcattttat cgagatttgc
     6121 tcttacatga ctgttgatga tatcaatttc ggcaatactc ttttcaagct ctgctgttga
     6181 ttcatcctgc aagctctgaa cggtcatttc ggcaattctt aaatcattgg agagcgtatc
     6241 tcttttatcc gaaagctccc gtaattttac ctgtaatgca gtgatttcat cgttcaccgt
     6301 atcatatgct cttttgatat tggcagcatt ttcacgctta cgctgatttt cgccgttccg
     6361 tgcaaggatg tcctgctgtt cttttatgag atcagccgcc gaaacaaggt ctgtaggtgc
     6421 ttcgggatag cttttcattt ccgaagcatg ttttctttta ctgtcggcaa gctgtcctat
     6481 gtatctgcgc ttgttgtaga tttccgattc tttctgttca agctcaaaca gcttatctcc
     6541 gacaccgatt atctgtaaaa gaatatctgc cttttcctta ctgcttgatt ccataaactt
     6601 cggaatattt aaagcaaatt gagaaacaaa ttcattaaga agctgctgac ctgcacggtt
     6661 acccgacgga tcaataacct ttaaatcgct gtttttacct ctgcgttcaa caaccaaacc
     6721 gtttgaaagc ttgatttgaa gaagtggcgg aatgtttgag ccttcacgca ttgctgatga
     6781 cggcttgaat ctatcgccac ccaaagccca tgcaatggca tcaagaacag aagttttacc
     6841 ttgattattt cgtccgccga ttaccgtcaa tccgttttcc tgcggttcaa cggcaacggc
     6901 tttaatacgt tttacatttt ctaattcaaa ctgtgtgatt tttaccatat ttgcctccta
     6961 cttgacaaaa actatttttt tgatataata gggttgaata aagtgtgtga tttttattca
     7021 accttagaac tcgttcagtt gcttcagttg ctgctgggcg ggttcttttc ctttatcagg
     7081 acattctctg cgttggcttc aaagtgtgcc tggcgctgtt nnnnnnnnnn gtcaagcgtc
     7141 tttgcaagcc tgacgatctc ggcatctcgt tcggcgttga tgttgtaaag cttgtccttg
     7201 attgcctgat atccgccgaa gaaaaacgcc gccgtgaaca gacttccgaa aaatcccgca
     7261 aaaaatatcc agaaaaacaa atccattgct tatccctcct ttccaaagaa atcgccgaag
     7321 cgatccatta ttgcctgttt gggtattctc catgagctgc cgaacttggc agcaccgggc
     7381 atttcgcctt ttcggcaaag ataattgacg ttatcggacg tgcaatgtaa aatttgagcc
     7441 gcttcctgtg ccgtgagaat atctccgtac tccgaaaact ttacgggtcg atacactggg
     7501 gatttacatc ttactgccat actctcacct cctcattttt atttggtggt tcataaaggt
     7561 tgtattgccg ttctgagtgt aattaccgtt tttcataatt gacactcctt gcattgtgaa
     7621 ttttcttcac tcgattgtaa ttcttccttg ttttgtgcta taatcgcctt gaaaggtggt
     7681 gattatatgc gtgagttttc ataccgattg aaaaacggta tgcctgacga agtggaacat
     7741 gtaatttaca tatatacctc cgcaggttgg gagtttaaag aattgttgcc ggctgacggt
     7801 attcctcagc aaattgtttt tacatggaat caagaccgag taccgtttta tcctcctatt
     7861 tacttacctg agtgataact atacttttaa agtctttcaa atcgtcactt ccgattgttg
     7921 taatcagctt caaatcggga ttggcggttt ttgcttcttc gtattgttct tcgctttgaa
     7981 agataattac cgtccctgca cggtctaatg taaatccgta tgatttttgt ttcatatttc
     8041 acctcctgtt cttgcatttg tatctaatta agatacatta tcttcaaaaa aaatgtgctc
     8101 aatttcgtca gcagtcaagt gatagcgtct tttcattgcc gacatctctg gctgcgtaaa
     8161 acttgcacga ccgtttatct ttgcagaaag cgttgttctt gatatgccta acgcttctgc
     8221 caacttatac tgtgtgtcac cgttaagaca cataacgctc atcagcattc ctttgttcat
     8281 tgtatcgcct cctgtatctt tttaagatac ttttatttta gcacacattt tttatttgtc
     8341 aacccttttt gtatcttttt tttaactttt tttaaaaaat attgacattg ttctaaaaat
     8401 gcgatacaat tcgtttatac aaggtggtga atataaatga ccgttggaga ttatataaaa
     8461 gagttgcgaa cttcaagagg ttattctcag gagcaattag gtaaaatgct tggagttcaa
     8521 agagccgccg tccaaaaatg ggaatgtgga gcagtacaga atcttaaaag agaaacaata
     8581 aagaaactct cggaagtatt caacgttcct gcctcttcct ttattgatag tgtttgtgat
     8641 ccagcatata aacctaccga tgaagatata aaattcgccc tctttgacgg tgcggacaat
     8701 ataacagatg aaatgtatga agaagtcaag cgctttgccg aagccttggg tacttcatca
     8761 tgctacctac ttgacggtaa agaaacatca aaagattttg tttcatcttt tgctttaacc
     8821 gaacgagaaa aagcgttagt aattgcatat agaaatcacc ccgatatgca gaaatccgtt
     8881 gataagcttc tcggcattga gtcggaggaa aagctttata taatagaaaa agccgcccgt
     8941 aacggagata acactccgct tgtagttacg gacagcaagc tcaaggaact tgagaattta
     9001 cccgaagttc ctcttgagga tggttaaatt ttgttgtgtt ttataaaggt ttcaaattgt
     9061 gagtagactt tctgctcaag cggagatatc aaaaagcgat tgcgcttgta aagctcgttc
     9121 attctttccg ctctgtactc tgcggcggtt cggctgatct tgcaaagttc ttgtatttga
     9181 tcggcggtcc gaacattgca cccccacagc acgcaggcgg gagctaacag acggcttgca
     9241 aacacgtttg ctgcttgttc aatcggatta tcggtcggtg acggctctcg gtttacaagc
     9301 tcgaacttcc cgacgtgatt gagaataatg tgcccgattt cgtgagcaac agtaaacctt
     9361 cgacgctcag gcggaacagc agagcttacg aaaatgtgtg gtatgttgtt aatgtagcag
     9421 ctcatgccgt cgctttcatc ggtcggctcg taatagtgca gcttgattcc aagctcggcg
     9481 cagattttga cggtgttgat cggcagttcc gaaatgccta aatcaatcaa caactgccat
     9541 accgagtctc ttgcattttg atagtcttta taattcatat aaaatcacct cacttaaaat
     9601 tttaagtgag gcaaagtgta aaatctacag gtaagttttg gtaattactt gccgtgaaaa
     9661 aggagggtga aaatgagagg tgcgaaaaga aagccaagca agaaaacgat cattataatt
     9721 gttgctgtcg ttttatttct gggaataatc ggttcgtgca atgattcgct gcaaaacaaa
     9781 aacgaggaac ccgaaacaac agcatcaagc actacagttg ttacaacaac gtcaaggcca
     9841 acaacggtaa caactaccga aacaacaaca gagccgacaa ccgaggaaga gactgagcct
     9901 aaaagggaaa tcgttcatac tcaggcatct tacgattata tcgtaaacac aaatacaggc
     9961 aaattccatt acagctcttg ttctcaggcg ggtcgaatca aggaagagaa taaagctgtt
    10021 attcatggaa cttccgacga gctggaaagc atgggatata ctccttgcgg caaatgtaat
    10081 ccataataaa aaagccgctc gaggctgctt ataagagaaa ttgattagct tagttcataa
    10141 ccgccttgcg gtgtgataaa tgactttaat tgaaagggtg ttaaaaatgg gatttattcc
    10201 gatggtttgt ccgcaatgcg gtgcacaggt tcagttggac gattcgagag aatttggctt
    10261 ctgttcctat tgcggcacaa aaattgtgca ggaaaaggta gtcgtcgaac atcgtggaag
    10321 tgttggcgtt gaccattccg gtgaaattga taatcttctt cgcagagctt ccgaatatat
    10381 gcaaagaggt gacaccgacg gagcagagat ctattacaat cgtgttttag acttagattt
    10441 tgataacgag attgcacgga acgcaatgga gagattaaat caaatcgtaa aagaaccaaa
    10501 tcttttcatt acagcaacaa caggaaagct ttacaataaa aaggcaagta tccgtataaa
    10561 aatcgacggc atagattacg gtacaatttt taacggcaat acaggctctt ataaattaaa
    10621 tgtcggtaca cacaaaatac gattaaaaat taacagcgta cctttttaca aattagattt
    10681 taatgttgaa attaaaaatc gatttacaaa actacaatat gtcgctacat gtaaaatcgg
    10741 caacgtaata gaaataaaat aacggagata ttgaaaatga tatgttcaaa gtgtaaaaag
    10801 gttattccca acggttcagt acagtgcccc gagtgcggcg cagatcttaa atc
//
LOCUS       DAWBSW010000056        10659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1332_length_10659_cov_2.831479, whole genome shotgun sequence.
ACCESSION   DAWBSW010000056 DAWBSW010000000
VERSION     DAWBSW010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_10659_cov_2.831479"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2675..2684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6043..6052
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2982 a         2403 c         2663 g         2591 t
ORIGIN      
        1 tcgtcgcctt acttttggcg taacgtaaaa cggaggttta atatggcaaa atatgcaatc
       61 ggtgttgatt tcggtactct ctcgggcaga gcactgctcg ttgacgtcaa aacaggtgag
      121 gagcttgcaa cctcggtcct cgactaccct cattccgtta tggacgaaga gctcccgagc
      181 ggcaaaaagc tcggcgcgga ctgggcgctt caggaccctc aggactatct cgacgtgcta
      241 aaaacaacta ttcccgacgt tataaagcaa agcggagtta atgcggagaa catcatcggt
      301 ctcggactgg atttcaccgc ctgcacagtc atgccggtca agaaggacgg cacgccgctt
      361 tgttttattg ataaattcaa ggacgagccg cacgcttatg ttaagctttg gaagcatcat
      421 gcggcacagg ctcaggcaac aaagctcaat gagattgccg aaaagatgaa tcaggactgg
      481 ctcaagctct acggcggaaa gatttcctcc gagtgggcta tcccgaagct ttggcagatt
      541 cttgacgagg ctcccgagat ttacgacgag gcagatcgct ttatcgaagc ctccgactgg
      601 gttgtttggc agatgtgcgg ccatgagatc cgcaattcct gctgtgctgg ctacaagggt
      661 atgtggaata agaaaaacgg atatccgtcg gacgagttct tcaaggctct cgacccgaga
      721 atggagcatg ttatcgacga gaaattctcg agagatattc ttccgctcgg ctcaaaggca
      781 ggcgaggtca cagccgagtt tgccgcaatg acgggactaa aagagggtac ggctgtcgcg
      841 gttcctatta ttgacgctca cgttttcgtt cctgccgtag gtatcacgaa gccgggcgaa
      901 atgctcgcta tcatgggcac ttcaacctgt catatgcttc tcagcgagga ggaaattccg
      961 gttccgggta tcagcggcgt tgttgccgac ggtattctcc cgggtcttta cgcttatgag
     1021 gccggtcagt gctgtgtagg cgaccatttc cagtggttca ttgacaactg cctgcccaag
     1081 agctactatg acgaggctga gaagcaggga gttaatatac ataaattcct tcgccaaaag
     1141 tgcgagcaga agaagccggg cgagagcgga cttgttgcgc ttgactggtg gaacggcgtt
     1201 cgttcggtgc ttgttgacgc cgatctttcg ggcatgatgc tcggaatgac gctaagaaca
     1261 aagcctgagg atatctacag agccttgatt gaagcaacgg cttacggtac aagaatgatc
     1321 attgatgttt tccgtgagaa cggtgttgac gttaactcct tctatgccgc aggcggtata
     1381 agccagaagg atccgatgac tatgcagatc tatgccgatg ttatcaatat gccgataaag
     1441 attgccgact gtgagcaggg cggtgcgctc ggcagcgcga tctacggcgc atgtgccgca
     1501 ggtgctcaga agggcggcta cgacagcctt tatgacggtg ctgcggctct cggcaaggtc
     1561 aaggataagg tttactatcc gattccggag aacgttgagc tttacaacaa gcttttcggc
     1621 gagtataaaa tcctccacga ttatttcggt aggggagaga acaacgttat gaaacgcctc
     1681 aaggagctta aaaattcctg attgacagca tttttatata gtggtaatat aatttgaatt
     1741 ttaaatatag gagtctttta aatgagcatc ttagatcttg aagtaattaa aaaatttgca
     1801 cttacctgca atgacggcta cgagcagggc tggcacgagg gcaacggcgg taacctttcc
     1861 taccgtatga cagccgaaga ggttgaggct tgcaagcctt actttacata tgacagaccg
     1921 tgggttgacc tcggtgttca ggcggataac ctcaaaaagg agtatttcat ggtaacgggc
     1981 acggggaaat ttttccagaa cgttaaaaga gatcctgccg caaatatgtg tattcttgaa
     2041 atcaacgacg caggcgacgg ttacagaata gtatggggtc tagttaacgg cggcggcccg
     2101 acaagtgaat ttccgaccca ttttatgaac cacagcgttc gtgttgaggc aacggacgga
     2161 gcctgcagag ttatctatca tgctcatccg gtcaatatta ttgcgcttac ttttgttctt
     2221 ccgctcactt ccgaggcttt ttcaagagtc ctctgggaga gcatgaccga gtgtccgatc
     2281 attttcccga acggcgtcgg tgttgttgaa tggatgattc cgggcggagc cgagatcgct
     2341 cacaaaacct gcgagcttca gaaaaagtac gacgctattg tttgggcgca tcacggactt
     2401 tttgtatcgg gcaacacatt tgatgccgcc ttcggactta tgcacacgat tgagaagtcg
     2461 gcagaaatct attgcaaggt tctcagcacc ggcaagccga tcgctcagac aattcccgat
     2521 gagggactta tcaatgccgc aaccgcttac ggtaagaagc tcaatgcgga gcttttgaag
     2581 taattacgtt ttgtaatgtc aacattacaa gctgtctgtt tacaaatcga caaaagtcaa
     2641 ctataataac tgcaatattt gaaatgcgtt gaagnnnnnn nnnnatatta agcattatat
     2701 ttctgatttc agagagtcgg cggcaggtgt gagccgatat ggaaatttaa ttgctgtctc
     2761 gtatccgagc agagctcccc aaccgttttc actgtataag gagctacggc aagcaccgtt
     2821 atcacagctt aggatttgtc agaatccgaa gaggccttat ttaggcaatg cgggtggtac
     2881 cgcggtaaac atgttttatc gtcccggaag ttttaatact tccgggactt tgttttttat
     2941 atatggcgat accgcatata ttacggtatt gcaatacata acaaaaagga gaatcgttat
     3001 gagaaaggtt attttatccg atgtgacgct cagagaaagc gagtcgtcag ccagccattc
     3061 cttaagcttt aaggagaaga tcgaaattgc aaagcagatg gacaagctgc actgcgatat
     3121 tatcgagatg ccgaaaatta agaacacttc aacagatgtt cttcttataa aaacaatcgc
     3181 ttcgcttatg aaacattcgg aaatagccgt tgatgcaggc tacgatgcgg caggcgttga
     3241 aacggcatat aactgtgcaa agtgtgcaaa gaaattttct cttaaggttt ctctcccgat
     3301 gtcggccgtt cagatggagt tcaagtgcgg caaaaagccg ccggttgtca tgggcatggt
     3361 tactgagctc atctccgaat gcaagaagta caccgacaat attgaattct ctgccgagga
     3421 cgcggtaaga gccgaaaaac cgttccttta tcaggcaatc ggcgacgcga taaaggcagg
     3481 cgcaacgaga gttaccgttt gtgattccga gggcacaatg tttccgtcag agttcggtgg
     3541 atttatctcc gacattttcg agaacgttcc gtcggctaag gatgtcactc tcggcgttga
     3601 ggtcgttgac tcaatgaaca tggcttcggc ttccgcatgc gccgcaatcg aagcgggcgc
     3661 aacgcaggtc aaggtcacgg cagataactt caatttcccg acggttgaaa aaatcgcgaa
     3721 gatattcacc ctcagaggtg attcgataaa ggtttcgagc aagataagga cgacagagat
     3781 acgtcgagta acgggtcaga taaagagaat gctcaacacc tccaagagcg agaatacacc
     3841 gtttgaaaca ggtgtgaatg aggacagcga gaccctcact ctcgataaga acgatacgat
     3901 cgcaaccgtt atcaaggcgg tcaagaagat aggctacgac ctttctccgg aggataacgc
     3961 aaatgtttac gaagccttca agcgtgttgc ggtaaagaag gatttcgtcg gaacaaagga
     4021 gcttgaggct atcatcgctt cctcggcttt gcaggttccg tcaatctatt cgatcggcag
     4081 ctacgttatc aacagcggca acattatcac cgcaaccgct aacatcaagc tcgtcaagaa
     4141 cggcaccgag atcagcggcg taagcgtcgg cgacggcccg attgatgccg ctttcctcgc
     4201 aatcgagcag atcatcggcc atcactatga gctggatgat ttccagatca aggcggttac
     4261 ggagggacgt gaggctatgg gtcatgctct cgttaagctc agaagctcga ccggcaagct
     4321 ctattccgga acaggcattt caacagatat catcggcgca agtatccgtg catacgtcag
     4381 cgcactgaac aagatagcat atgaggaggg ttaattgtga aattagcttt ttctacaaaa
     4441 ttccacaaga ataaaagtct tgatgagttt ctcaatatgg cggacgagct gaaatacggc
     4501 ggaatcgaga ttcacgattt ccgagacccg atgttctcgg gcagaacctc accgttcaac
     4561 cccgacatgg cttcttcaac caaaagaaag ctcattaact caaaaaagag catcgtttgc
     4621 ataaactcaa actgcaatgc cgccgacacc tcggacgaaa atgttgaaaa aatgaattcg
     4681 gcaattgccg tggcaagcaa tatcaacgtt ccctatataa agctttatgt cgagggcggc
     4741 gaggatgctt acgacaaggc gatagagttc atcggcaagg caatagcaga gggcgaaaag
     4801 cataacgtaa tcgtgcttgt tgaaacgacg ggaatttttg ccgacacaac aagactttgc
     4861 gacttgatga actacttcgc aagcgacgag ggcacaatgc ctattaagga tatgatgaac
     4921 gctctcggtt caatcaatta taacggcttt atttcggttg aatggaatcc cgaatggttc
     4981 aagtcgattg acgatctcga aatcattttg acccactatg agagctatat ggagaccgtt
     5041 aaagacgaaa caaacaagaa aaagtattac tataacaaga cccatacagg caaatttgta
     5101 tggaaaaaag aggagcttat tgaggagact tttccgcagg ttcttgacag aatggttgag
     5161 gaatttcccg atcagcttgc attcaaatac acaacgcttg actacacaag aacttatgca
     5221 cagttccgtg atgacgttga tacctttgca agagcgctta tcgcaatggg cgtcaaggca
     5281 ggctctcatg ttgccgtttg ggcaaccaat gttccgcagt ggtatatttc attctgggca
     5341 acgaccaaaa tcggtgcagt tctcgttacg atgaacacag cctataaaat ccatgaggcc
     5401 gagtacctgc tcaggcagtc agacactcat acgcttatta tgataaaagg ctacagagat
     5461 tcgcactatg atgagatcat ggctgagctt tgccccgagc tggagcacac aaagagcggc
     5521 gaaaagctcc acgcaaagag actgccgttt ttgagaaata ttgttactgt cggctatgag
     5581 cagcagggat gtatgacatg ggagcaggca gttgacaagt ccggagagat tcctgtcgag
     5641 gaggtctaca gaatggcttc tctcgtcaga aaggacgatg tctgcaacat gcagtatacc
     5701 tcgggcacaa cgggcttccc caagggcgtt atgctgacgc actataatgt tgtaaacaac
     5761 ggcaagtgca tcggcgacag aatggatctt tcaacggcgg acagaatgat gattcatgtt
     5821 ccgatgttcc attgcttcgg catggttctt gccatgacgg caattatgac acacggcgga
     5881 acggcacttc ctttgcctta tttctcaccg aaatcttcgc ttgcatgtat caattccgag
     5941 cgcataacgg ctttccacgg cgttccgaca atgtttattg caatgctcca gcaccccgac
     6001 tttgaaaaga cggatttctc atacatgaga acgggtatca tgnnnnnnnn nngctcgccg
     6061 tgcccgattt caaagatgcg tgaggttgtc gataagatgc acatgaccga aatcacaatt
     6121 gtttacggtc agacggaatc ctctcccggc tgtacaatga gcagcacgga tgatcccttg
     6181 gaggaccgcg ttgcgacggt cggacatccg cttccaaaaa tcgaatgtaa aatcgtcgat
     6241 cccgatacgg gtcttgagtg ccccgacaat gtaacgggcg agtttgttgc ccgaggctac
     6301 aatatcatga agggctatta taaaatgccg aaggctacgg caagcgcaat tgataaggac
     6361 ggctggcttc atacaggcga ccttgcatgc cgcactccgg agggtaactt cagaatcaca
     6421 ggcagactta aggatatgat tattcgcggc ggcgagaata tctaccccaa ggaaatcgag
     6481 gagtttatct atactcaccc gaaggtcaag gacgttcagg ttatcggcgt tcccgatgag
     6541 ggactcggcg aggaaatcat ggcttgcatt atcctcaagg agggcgagac catgaccgag
     6601 caggagatga agggctacgt tctttcgcac atggcaaagc acaaggtgcc gaagtatatt
     6661 gactttgtga ccgagttccc gatgaatgtt gccggcaaga ttttgaaata caagatgcgt
     6721 gaggacgcta tcaagaaata cggcctgcaa aaggcaaatt ccgttgtaac ggcgtaacat
     6781 tacaaaatgt aatgttaata ttacaaataa tctcttgaaa aatcaagaaa actatcttat
     6841 aatgtaccgc aacaggtaca aataacctgt tgaaacaaag gctgtgacga agacggttgc
     6901 gtttatgtcc tttcagagag tcggcgtttg gtgtgagccg atagcgagat gaatgtaaaa
     6961 cccatcactt ccgagccgga gagatgaaac aacagtagtt tctcccgtga caactgcgtc
     7021 agtgttgagg gattgtcaga tcctgcagag aggcgagaag caatagcaga tcgcaatttg
     7081 agtggtaccg cggattatca aatccgtctc aaagttctaa ggactttggg acggattttt
     7141 tgttttatcc gaaatcggtg ccggtacatt acggaggtaa aaattatgtc aaatcaaaca
     7201 gaaacaaacc agctttatga agtggcggaa cgtatccacg aaatgagaga catatgtgcc
     7261 tttaccgttg aacagatggc ggaaaagacc gaggtctctg ttgaaaccta cagactttat
     7321 gagagcggaa cggtagatct cccgttcaca tttattcaca aatgtgcgct tgcgtttgac
     7381 atcggcatta cggatcttct tgaaggtcac agcgccgtac tttcatctta caccgtcaca
     7441 agaaagggca agggtcaggt taccgccagt gaaaacggaa tcgagattca gaaccttgcg
     7501 ccgaaattca gaaaaaagct ctccgaaccg tactgggtta gatatgagta tgatgcagag
     7561 cttgaaaata aacctattca cactacaact cacagcggtc aggaatttga cctcgttatc
     7621 agcggtactc ttaaggtaag agtcggcaat catgaagaga tccttcacga gggcgacagc
     7681 atctactata actcgtcaac tcctcacgga atgatagcca ttgacggcag agactgtctt
     7741 ttccttgcaa tggttatggc aagtgacgaa cctgttcaga atattttgca tgaaagagct
     7801 gttatgggcg ttaaggctaa gggcagctac gtttgcgaga agtttatcga cgcaaccgag
     7861 gacgagaacg gcaaccttgt ttcaatcgac ttcaatcacg aggacgaatt taactttgct
     7921 tttgatatcg ttgataagat tgctaagaaa tcacccgata agcgtgctct tgttcatgtt
     7981 gaccgtgaca agacagagag aatcttcaca ttcaaggatg ttaaagaaca ttcggctcag
     8041 gcggcaaact acttcaagtc gctcggtatc aagaagggcg acagagttat gcttgttctc
     8101 aagcgtcact atcagttctg gtttgcgatc ctcggtctgc ataagatagg cgcaattgca
     8161 attccggcaa caaaccttct tgttgatcat gactttgagt acagatttga agctgcaggc
     8221 gtaacttcaa ttctttgtac ggctgacggc gacacagccc atcaggttga tatcgccgat
     8281 tcaaagacac atactctcgt aaacaagatc atcgtcggcg gcgagcgtga aggctggcac
     8341 aacttcgaca gcgaatattg cctgttctca agacgctaca gacgtgagga ggacgcaccg
     8401 tgcggaaatg atccgatgct catgttcttc acttcgggca caacgggtta tccgaaaatc
     8461 gcaacccatt cctacaaata tccgctcggt cactatatca cagctaaata ttggcactgc
     8521 gtaagcaagg acggacttca tcttactatc tccgacacag gctggggcaa ggctctttgg
     8581 ggcaagctct acggacagtg gctctgcgag ggtgccgtat ttgtatataa cttcgatcgc
     8641 ttcgatgcat cggatatcct tccgatgttc gctaagtatc atataacaac gttctgtgca
     8701 cctccgacga tgtacagaat gatgataaaa gaggatctcg gcaagtacga tctttcttct
     8761 atccgtcacg caacaacagc aggtgaggct ctcaaccccg aggtattccg tcagttctac
     8821 aatgctacgg gacttgagct tatggagggc ttcggtcaga cagaaatgac cctcggaatc
     8881 gcaaccctca cgggtatgac tccgaagccg ggctcaatgg gcaagccgac accgctctac
     8941 gatatcaaaa ttctccgtcc cgacggaacg gaagccgacc tcggtgaaac cggcgaaatc
     9001 tgcgtcaata cctccgaaaa ggtaccgtgc ggaatattcc tcggctacta tagaaatcag
     9061 gaaaaaacag acgaggtttg gcatgacggc gtttatcaca caggtgatat cgcatggcga
     9121 gatgaggacg gttacttctg gtatgtcggc cgtattgatg acgttatcaa gtcttccggc
     9181 taccgtatcg gaccgttcga gatcgaaaat gttattatgg agcttcctta tgttcttgag
     9241 tgcggcgttt ccgctgctcc agacgatgta agaggacagg ttgttaaggc atccattgtt
     9301 cttaccaagg gcacagaacc taccgaggag ctcaagaaag agattcagaa ctacgtcaag
     9361 aagcacaccg ctccttacaa gtacccgaga atcgttgtat tcaaagatga attaccgaag
     9421 actataagcg gcaagattat ccgtaatcaa ctgtaaaatt gcccatggca cgccgatttc
     9481 ggcgttgaca ggcttagcaa tacacatagt attgcgtcag cctgtcgcct tgaactcgac
     9541 gcaccctgaa caatttgatt taaggtatgg cacattgctt tcttcgttgg tgagcctcga
     9601 aatacacata gtattccatc ggctcaccgc cttgaaatcg atgcaccctg aacgcttggt
     9661 tctaaggtat ggcatgccga ttttagaaga aatataccgg cttccccttg agggaaggct
     9721 gattagttcc tgtaactata ataatttttt atcaaacttt cccattgctg cgtggtgcga
     9781 taaaggaaca attcgtaggg gtcgatgccc acatcgaccc aatgacaggg gaaaaatttt
     9841 ttcggcgctc ccatatgagg gggtttgcta aaatttatca tttaatcatc aaactttccg
     9901 aggtgaaaat atgaagcgta taacgctttt tgccggtcat tacggcagcg gaaaaacgaa
     9961 tattgccgtc aattatgctt ttaagctgag acagcagggc cttgaggttg cggttgccga
    10021 ccttgatatc gtcaatccct atttcagaac gaaagacagc gaaaaggaga tatattcggt
    10081 tattgacgac cgttccgaat ttgccgtcat ggatatcggc ggagacgaca ggggagctta
    10141 tgcgctcggt cgctattcgg aaagcatagt ccgtgaaaat gactatgaaa tgtaccttgt
    10201 aataaacatg tacagaccgc tgacaaggga cgcttacagc actcttgagg tcatgaacga
    10261 aatcgagaaa gccgcttcaa tgaaatttac cgctataata aacaattcaa atctcggcga
    10321 agagacgact gcacaggatg ttctcgattc actcaaatat gccgacgagg tttcaaagct
    10381 tacgggactt cccgttgcga tgacgaccgt taagaaaaca ctctataaag aactgtacgg
    10441 aaaagtggac aatatgcttc cgcttaccct gcaggagaaa ataaattagg aggaatgtca
    10501 aatggctaaa gtaacattca aaacagacct ttgcaagggc tgcggacttt gcgtgaacgc
    10561 ttgtcccaag ggaatagttc acttgtcaga gacaacgctg aacaacaagg gacatcatcc
    10621 cgcagttgtg acagagcagg aaaaatgtat aggctgcgc
//
LOCUS       DAWBSW010000057        10243 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1385_length_10243_cov_2.901943, whole genome shotgun sequence.
ACCESSION   DAWBSW010000057 DAWBSW010000000
VERSION     DAWBSW010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1385_length_10243_cov_2.901943"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2947..2956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6712..6721
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2825 a         2321 c         2342 g         2735 t
ORIGIN      
        1 cacagtcacc gtccgccgca atcataccgt gggagcatag tctgcacgca ggagtgatat
       61 cctcagtatt cacaagtttt tttctcattt tatcagtcct ttcggaatga agtatccgaa
      121 aagtatttta tcatattttt ctgtcggttg caaggttaat gtcgattatg gtaaaagcac
      181 cgagaacaag catggcggcc gaggccggaa cggagatcga ataaggcttt ataatcacgt
      241 ccgagaagct tgaaaacacc tgcgtttcac acgggaaaag cttgaaaagc aaatatgcaa
      301 tcggcagggc aacaaggcaa ttaacagctc ttgaaagcca aaattcgggg aaagatatgc
      361 cgatcttttt gatgtaaggc agaagctgca tgtgaacggc aaagcccgac cagcaaagaa
      421 cgggagctaa tagataaacg ggcagatgct cggcggcaaa aacgcagcct cccgttacct
      481 cggacattac aacggcggtg ttgaacagtg gatttcgttc ggcaagtttt atcaaaaaag
      541 gattgaacgc cgaaaaaagt aaaatccatg cgcatatatt cagcatggct cccgttgcac
      601 agaaaacgga gtccgtaaga acggaagtat caatttcaaa tttttttatg gctttttctc
      661 tgcctttttt atctgcaaaa aatcttgaaa aaaaaccgat aatcagcgcc gcgagagttt
      721 gagaagcgaa taaaataatc ccgcactttt tgcttgaaag cattgtcgtt ccgactgcgc
      781 ctacaacaaa ggcgggaccg gaatttatac aaaaccacac cattcgtttt gcctgcgaag
      841 ccgaaagaga attgttctcg tataactggg aaatcatttt tgctccaaca ggaaatccgc
      901 cgacaagact cataaatatt gcgcacgccg agcagcccgg gagcttgaaa aacttctcgg
      961 tcagaggaga taaaaacctg ccggcagcat tgccgagatt ggagcggaca atgaaatcag
     1021 aaagaatcat aaaaggaaac agcgatggga taaccgtttc gccgcaaaga aaaagtccgt
     1081 ttcgcacacc gtcacccatt aaggacggat caaagaaaaa aagtataata aaaccgatcg
     1141 aagcagtcag cgaaaaaatc agttttctgt aattatccaa ggcaatcacc cgtaaatata
     1201 tatgagggga aatctttagt ttatgatagg gtgattatga tgccaaaaga ccgcagacaa
     1261 tataacactc cgtttgcaca caggatttgc gaacggctgg aacttccgga ggatgttttt
     1321 agaaattgca gtcatattga gcttgtttca aactgctgcg ctatggtaga cggatgcaaa
     1381 agcgttatgg aatacgacga cacggttata aagctctgtc tggggaaaag cagcgtaaaa
     1441 ttcacgggat acgatttaac gataaaatcg ctttcccttg agcaggcgat gatcgaaggg
     1501 aaaattattt cattggaatt ctgcgaatga gggcttgtta tgatttttaa tctttttcat
     1561 ttaatattcg gctgtgtttg tttcacggcg agcggcggct ttgcagaaag gtttataaac
     1621 gtgtgcaccg ctaaaggtat ccagctttgg gggataagaa aaaaatcata cggtctgaca
     1681 gcgtgcacga caattgaggg atacaaaaaa ataataaatg ccgcccgtgc aagcggcatg
     1741 aaagtacgaa taaaaaagaa agtcgggctt ccgtttgtta tccgtaaata tgagagcctc
     1801 acgggacttt ttgtcggttt ttttgttgtg cttttaattc tttcgataat gtctaatcac
     1861 atttgggtcg tcaatattca cggaaacaca acggttgaca gtgaaaaaat cgctgatgtt
     1921 ttttcagacg caggcttaag tatcggtgta aggaaaaata aatttgacgc aagcgatatc
     1981 aattccgatg ccgtgctgag catcggcgag ctttcatggg cgtcgatcaa tattgacggc
     2041 tcggttgccg aaatcgaggt cagagaagca atgcacagac cgagccttga aaaatattac
     2101 ggcacgtcaa atattgtcgc atgcaaggac ggtcaggttg aaatcatcga accgtacaag
     2161 ggagccgccg cagtcaaggg cggacagacc gttatggagg gcagtctgct cgtcagcgga
     2221 ataagtcgtg tcaaaagcgg aatgtccgta ttcaacgacg ccgacggtta tattgtcgca
     2281 aggacaaaaa taaacgtcga cgttccgatt gaaaataatt ctgttgtatt aaagccgaaa
     2341 acgagaaaga tatattctct cttttttctc ggcaaagagt tttcagtttc aaaaaagcgt
     2401 aaagccgata cggtttaccg ccacaaatca tggctttaca ttcacggggt aagaatgccg
     2461 ttcggtattt tttatactca gtacaccgat tttgaagaag aaaacaaatc ggcggacaaa
     2521 gaattatccg agctttcggc tcttaacgat tatgcactga aaagctacaa tgaaacgctc
     2581 catgcacaga ttatttcaca aatggttgaa ataaaaaacg gcacggtgaa gggtgaatac
     2641 agtgcttttg aaaatatcgg aaaacgggtt ccgtttgaaa cggaggaaac accgcaggag
     2701 tcagccgatt aagcaagctt caccttttgc agcagctttg cacggtcaag cgcaagctcg
     2761 gcaaagatta tctcaacaac gctcatcacg acgaactgta tcgctcttgt tgcaagcagt
     2821 gcggtgaaag cctttgtgta aagaattgag atgaacagcg tgttgagaag cagttcgcaa
     2881 acaagctgag tggagagcac ggcaatgacg attctcggga aattgatttt tttgtaaaga
     2941 agcagcnnnn nnnnnngaag cagcccgaaa acaagtcccg ttattgccgc cgtgacggtg
     3001 aagccgggaa aataggcacc tgtcggcata agtatcgctc cgataaagtc aacgagtgca
     3061 ccgacgacag ctccgccgat aggtcccgag atgcaggcgg cgagcatgac ggcaacaaac
     3121 gcaaattcaa tcttcgtgtt ccaaagattg accacgggaa ccagtctgcc gagtacaacg
     3181 cctgccgctg tcagcagtgc gagcacacag atttgttttg tcaaattttt cttttttgtg
     3241 ttcataaaat aaaaacctcc ttattacccg ttttgaataa taaggaggac aaaagtcgca
     3301 ttattactca gcgggatgcg acagaagaac cgcaagaaat cttttcgtcc gacggacaac
     3361 tccccgttcc gccggcactt aacgctcatc tgtctactct gaacagtgct attatatacc
     3421 ttatattaag tttgtcaata gttttgcaac cgttaattta attatttatt aaatccttcg
     3481 accttcatgt agtccttgga cttatatttg atgaataccg tcagcgccgc cccgaacgga
     3541 acagcgagaa cggttaaaac ctttttcgga gattcagaaa taaaatgctt gtttttcatc
     3601 agaatcgagc ttgaaacata gtgaatacag tttcttataa cgtccttagg ctggggaaag
     3661 gcggtcatat cgaattttct cagaaaagca aagccctgcg gattcctgag atactggcgg
     3721 tacatattcg tgcttgatcc gtcagccata tattcgacca cacaaacggg ttcattcata
     3781 attatcaggg tgtaatcaag gtcggcaagc gtatatttat aagagagcgg aacatatttt
     3841 tcacccggaa aggtggggta ggggggatag tttttcatta tatcggttcg gtagattatt
     3901 tttttatcgc cgcgtccgcc tcgttgatag taggagtaga acgtcgtgtc aaaccgatcg
     3961 gtttcaagct ccttgcctat cacgttgccg ttatcaaaaa tatcgagcgc aagaattccc
     4021 gcatatttat cactgccgtg tttcctccag cagttcacga tcttttcaac agcatcgtca
     4081 gtcatataat cgtcggagtc gatacaggtg ttcagctcgg tgtcgatatt ttcgtaggcg
     4141 gtgttgtgcg ccgtgtgcat accgccgttt tctttataaa catatttaat ttcaaatccg
     4201 ttatcgggca ccgtctgcca atgttttata agctcggctg tattgtcggt cgagccgtcg
     4261 tcaacaacaa gccatacaaa atcctttagt gtctgacggc gcaggctttc atagcatttg
     4321 tgaattgtgt aggctctgtt gtacgccggc gtgaacaccg ttagggtttt cttttgcgtt
     4381 tccatgtgat aatcctccga tagatgagcg ctgttctcat tttgcctcct ttgtttaaac
     4441 aagacgaggc tcgccctttt cgatacgtta atcccgaaaa aatccgccgg gtaaagcccg
     4501 acggattacc tttattcaat tatacaatca aataagcttt ttgtaaagag ctgtaatctc
     4561 ttcaacgctt gtgtctctcg ggttgcccgg acggcaagca tcatcataag cagactgtgc
     4621 aaggaacgga atatcctctt ccttaacaat ctctttgagg tttgccggaa ttccgacatc
     4681 gtgtgaaagc tgcttaactg cgtcaatagc tgccttgcga gcttcttcga tgctcatatc
     4741 ctctgtgccc tcaacgccca tagcctttgc gatatcacgg tacttatcgc ctgttgcagg
     4801 agcgttatac tccataacgg tcgggagaag aattgcgttt gcaacaccgt gcggtgtgtc
     4861 ataaagtgcg ccgagcggat gagccattga atgaacgata ccgagaccta cgtttgagaa
     4921 gcccataccg gcgacatact gaccgagagc cataccctct ctgccctctg cggtgttttc
     4981 aacggcacct ctgaggttct tggagattat ctcaattgcc ttgagatgga acatatccga
     5041 aagtgcccat gcgccctttg tgatatagcc ctcaatggca tgagtaagag cgtccatacc
     5101 tgttgcggcg gtaagaccct tcggcattga tgacatcatg tcaggatcaa cgactgcaac
     5161 aaccgggata tcatgtacgt caacgcaaac catcttgcgg ttcttctcgg catcggtgat
     5221 aacatagttg attgtaacct cggcggctgt gcctgccgtg gtaggaactg cgatgatggg
     5281 aacgcattta ttctttgtcg gagcaacgcc ctcaagagaa cgaacgtccg caaactcggg
     5341 atttgcaatg ataatgccga ctgccttcgc ggtatccatt gatgaaccgc cgccgatggc
     5401 tacgatacag tctgcaccga tttccttgaa cttggcaacg cctgcctgaa cattctgaat
     5461 agtcggattc ggtttgatgt cggtaaataa ttcgtatttg attcccgcat tttcaagaac
     5521 atctgtaacc ttctttgata cgccgaactt tacaaggtca gggtctgaag caaccataac
     5581 cttcttaaag cctctgccct ttacttcatc ggggatagca ttgatagcgc ctgcgccatg
     5641 gtaagaagtt tcgttaagta caaatctgtt catggtatta cactctcctt aataatcgct
     5701 tttctgcggt tgatttatct tttcggcgtg cttttgttat cttgcggtaa cgtttaccca
     5761 cgctttaatt atattataaa cttaaatttt gcggaaattg tgacattatt ttaacaacct
     5821 gtgaactttt ttaattcttt tacgaaaagt gttccgttgt taacggtgaa tcttatttcg
     5881 aatcccgcaa aattaaagcc gtaaattctt tcggggtcgt tctgatattg gggtctcggg
     5941 tcaagctcca gaaccttaat aagtgcagac tgcttgcttt cggggaacac ggataaaaga
     6001 ctgtcgggaa taacgacctt caaacgctcg ctgccgtctt ttgaaaatcc tccgacggcg
     6061 tcggggtgac agtcagcata gggtatatat ggcttgatat cgacaagctc cgttccgtcc
     6121 atcaaatcgg ctcccgaaat tataaggtcc attccgtcga ttctgtcgag tctcacacag
     6181 gaaagaccga tattgttagg acgaaaagga gaacgggtag caaaaacacc cattctggta
     6241 ttgccgccga gctttggcgg acgaacggtc agcgaatgat tttcggcagg tgtgttgaac
     6301 ttccacagaa gccagatgtg cgagaaaccg tcaagccctc ttattgcatc gggactgtgg
     6361 aattcgggct caaaaacaat tttgcccttc agttcctcga ccaaaccgct ctgtctcggg
     6421 attccgaact ttgtcggaaa gtccgtgtga atttttgcaa tcgttttcat agcgtcacct
     6481 tttcggcttt tttcaaaagt attatatcaa atcatgtgca aaaaaacaac gtcgactact
     6541 tgaaaaaata aaatagggga gtataataaa gaaaaattca gggagggata attatgtctg
     6601 actggaattc aaatcaatac atgaaattcg gtgccgagcg tactcagccg tcaatagatc
     6661 ttgtaaaccg cattgccgac ataaacccga atcgcattct tgatatcgga tnnnnnnnnn
     6721 nctggagcac tggcagatca gaggaaacga acagattctt gatatcggat gcggacccgg
     6781 aaacagcacc gataagctcg ccaagcaatt tccgaatgcc gacattctcg gtgtggattt
     6841 ttcggaggat atgttaaaaa aagcacggac aacctacccc gagcttagat ttgagcagag
     6901 ctttataccc gatgatctcg ataagcttga tggggaattt gaccttattt tttcaaatgc
     6961 ttgtattcat tggattccga atcatgagtc gctgctccct gcgattttcg ataagctcag
     7021 cgacggcggt actcttgccg ttcagattcc gtacattcaa aaggctccgt tttaccgact
     7081 tttaaatctt cttgttcaca ctgtcgaatg gaaagatctt tcttctatac ataattatca
     7141 caatcttctt cctgaggatt attatgacat tctcggcagg ctcagcgata atttcaatat
     7201 ttgggagacg acatattatc atacggttca gtcacataac ggcgtgatcg aatggtacaa
     7261 gggaagcggc ctgagaccgt atctcgactc tctctcggag gaaaaaagac ccgaatttct
     7321 tgcggatctg aacgagataa tttccgagaa ttttcctcgc agagccaacg gctcgataat
     7381 tttgaaaatg ccccgacttt tcttcacggc gaaaaaatga atataaaaca accgccgacg
     7441 agcaaatcgt cggcggttcg cttgttatta aggtttatgc ttgcttcttt ttattgatag
     7501 atcttctgat cgccttgcct atttcaaatg caggaaccgt tgaaagtgca agaccgaagg
     7561 aaataagaag ctcttccgta ctgaatgtac ccggctcgat tccgaatacg gtgcaaagac
     7621 ccggaatata gatagccgcg agagtcaatg ccgttgttgc aaagaatgca cctacaagcc
     7681 accagttcat ttttttcatc atgtctgccg agaagagtga agcctgacgt gaacgcatat
     7741 ttattgcaca catcatttca gcaaagttaa ctgtaaggaa tgccattgcc attgcgttga
     7801 cacattccgt gccgctgaag aaacctgcga ggctgtgtgt tgcaccgcct acgggttcaa
     7861 gccaatatcc tataatgtat gcggcaattt cgatgattgc aaggtaaatt ccctgccata
     7921 ccatgtcaac accggcaccg tttgaaaaga taccgtccgt tgtcgggcgc ggacgtcttc
     7981 tcatgatgtc accctcagct ttttccatac cgagagcaag acccgggaga gagtccgtaa
     8041 ccatgttgat ccaaagaaga tgaacagggg aaaggatagt gaagtgaaga atcgaagcaa
     8101 cgaacatgat tataacttca ctgagatttg ttgaaagctg gaactgaaga accttgagaa
     8161 cattgtcgta aatcttacgt ccttcttcaa cagcgttgac aattgttgca aagttatcat
     8221 cggcaagaac catgtccgag gtgccctttg taacgtctgt acctgtgata cccattccga
     8281 taccgatatc ggctgccttg attgacggag cgtcgttaac gccgtctccc gtcatggccg
     8341 taaccattcc gcgagccttc catgctttaa ctatgcgtgt cttgtgctct ggctgaacac
     8401 gggcataaac ggaatacttt gtaacttcct ctttaagctc ctcgtcgctg aatttatcaa
     8461 gctctgcgcc cattatagcc tgtgaagcat ctgtaatgat gccaagctcc ttgccgatgg
     8521 caacagccgt atccttatgg tcacccgtaa tcataacggg gcggatacct gcctgacggc
     8581 actcgtcgat agctgcctta acctcgggac gaaccgggtc gatcatgcct gtaagaccga
     8641 taaagataag gtcgttttca agtgcttcgg gagcgaagtc cgtaggagcg gaatcataat
     8701 ttttatatgc gcaggcaaga actctgagag ccttatctgc cattcttttg ttgtccttga
     8761 gtgcggcggc ttttatatcg tccgttatat caactacttt accgccgata agagcatatt
     8821 tacacttttt aaggatttca tcgggtgctc cctttgtgta ctgaacgata ccgtcggcag
     8881 actgatgaac ggttgacatc atctttctgc ccgaatcgaa cggagcttcg ccgatacgcg
     8941 gcatggcggc aacaagggta ctcttgtgca tattaaggga atttgcgtaa gcaacgaggg
     9001 cagcctctgt cggctcaccc gtaaccttgc cgtttgcatc aatttcagcg tcacagcaga
     9061 gagccattgc ttttgcaaga agctgttcgt catcgccgta atggtcaaca acggtcatct
     9121 tgttctgtgt gagcgtacct gttttatccg agcaaatgat ctgcgtacag ccgagagttt
     9181 cgacagcggt cagctttctg atgagagcct gacgcttact catggcggta actccgattg
     9241 aaaggataat cgtaacaacc gcaggaagac cttcggggat agcggcaacc gcaagagcta
     9301 tagccgcaat gaatgtatta agtgaagatt caccgagaag cttccatgta aaaggttcgt
     9361 ttgaaagtac aagcgtttca acaacgccga caacgaaaac gagaacgctg atgccgataa
     9421 caagctttgt gaggaagtga gaaagctcgc ccatcttctt ctggagcgga gtttcctcct
     9481 tttcggccgt gctgagagcg tcggcaattt tacccatttc cgtgttcatg cccgtgcctg
     9541 tgacaacagc tttgccgcgg ccgtaaacga ttgtagaacc gctgtaaagc atattttttc
     9601 tgtcgccgag ggcaacatcc tttgtgctgt ccttaagcat gagaacgtca accatttttg
     9661 taacgggaac agattcacct gtcagagccg cttcctcaac cttcattgag tgggattcga
     9721 gaattcggca gtcggcagga acggcgtcgc ctgcttcaaa gagaacgata tctccgataa
     9781 caacatcctc gctcttgaga acaacaacct cgccgtcacg cagaaccttt gacgttgccg
     9841 ctgtcatttg catgagggcg tccatcgcag cctccgcctt gctctcctga acgagaccca
     9901 ttatcgtgtt aatgataaca acaacaagga taacaaaaac gtcggcaaac tcacgaaccg
     9961 tcggctttcc gccggcttca ataactgcca caacgccctg aatggctgcc gcaacaagaa
    10021 gcatgataat catcggatcg gcaagagagt taataaattt tctgaacagg ctttcctttt
    10081 cggcttcctt gagtttgttt ttaccgtttt tctcaagtcg ttttgccgct tcggcagagc
    10141 ttaagccttt ttcggagctt tgagtctgcg aaagaacctc ttttgcattt tcaagataat
    10201 acttcaaaaa atgacctctt ttttgtaaca aaaaaccttt gac
//
LOCUS       DAWBSW010000058        10152 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1400_length_10152_cov_2.887392, whole genome shotgun sequence.
ACCESSION   DAWBSW010000058 DAWBSW010000000
VERSION     DAWBSW010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_10152_cov_2.887392"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3308..3317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3652..3661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6530..6539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9392..9401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2807 a         2239 c         2274 g         2792 t
ORIGIN      
        1 cggctcgtcg attttaacga acggcagcgg ctcaatacat tcggggtcac aagccgtttc
       61 accgatattg agatctgcaa tgcccgaaac gcaaacgata tctcccatca ttgcgctgtc
      121 gacctcaact cttttaaggc cctcaaactg gtaaagacga gaaattttaa cgttttcctg
      181 tttgccgtcc tgtttgcaga gaacaacatt ttctccacgg agaatctttc ctctttcaac
      241 tctgccgacg ccgattctgc cgatataatc atcataatcg aggctggaga aaagaatctg
      301 aagcgggccg tcaatatcac cgtcgggagc gctgatatgc tcaagaatag cgtcaagaag
      361 cggtctcata tcgccctcac gaacgtcagg atcaagcgat gaatagccgt cctttgccga
      421 agcgtaaaca acggggaatt caatctgatc ttcatctgcg ccgagctcga taaagaggtc
      481 aagcacttcg tcgataacct cggagggcct tgcaccggga cggtcaattt tattgacgac
      541 aacaagaact tttttcttga gattgagcgc ctttttaaga acgaatcttg tctgcggcat
      601 acagccctca aatgcatcaa cgaggagaag aactccgtca accatagtaa gaattcgttc
      661 aacctcgccg ccgaaatcgg catgacccgg agtatcgacg atgtttatct ttgtgttctt
      721 gtagtgaact gccgtattct ttgaaagaat tgtaattcca cgctcccgtt caaggtcgtt
      781 tgagtccatg actctttcct gaacctgctc gttctcacgg aacgttccgc tttgcttcag
      841 aagctcgtca accaatgtcg tcttgccgtg gtcaacgtga gcaatgatgg caacgtttct
      901 gagattttct ctttttgaca tagtatcacc tttcattatc ctgtggttcg agttcttgca
      961 aagtcccgca tgagatgtgt cgattttgca aaaattcgag ccacctttgt atatttattg
     1021 attattatat tttaatatat tatcggtcgg tagtcaagtt ttatcactta catttttatt
     1081 taagattttc aaaaacaaaa actgtgtagc tgacgtattt agccgaatag aattgttcat
     1141 agcaacgggt attaaccctt tgcatcaggt tcaatccgct tcagtcgtcc atggagccgt
     1201 ttcggataca cggcacaagg tttatttaga cataacattg cttttattca taaaaaatac
     1261 gttatatttt ttaaaattgt tgaaataaat ttctatgttt gatataatgg agaaaaatga
     1321 aaaaagagga tgattatgga ccctgtaaat acacctgtag tcaaaaagcg aaagaagaaa
     1381 aaacttaaca ctaagaaaat aataataatt ttgttgtcat tggcaattct gttctcggat
     1441 cttgccgtaa ttattcattt tacaacaaga gttacaaacg agtcatatgc caattgcgcc
     1501 gtgggtgatg taaacggaga cggaaagatc gatcctgccg atgcaacgct cgttataaaa
     1561 tacatggtaa atcaagagaa ccttttctcc aatcagatta agaattccga cgtaaatctt
     1621 gacggtgatg tcaattcgct tgatgcgctt ctgatactca gatattcgat cggcgatatt
     1681 tcgtcaatac cgtatgatga ggagaaagag atgctgctta acggctcgta caggagcaag
     1741 agaaccgtta aaggcggcag cttcacctca acggtgcaga tagtcaacga atgggacaac
     1801 aatgacggaa cccattccta tcaggtcaac ctgaccgtgc gcaacgactc ggacacagac
     1861 tcggattctt ggaaagccgc tgtttctttt gataaagaaa ttatttcgct gaccaaaagc
     1921 tgggattgca attcctttgt tgaggaacag agcataaccc tcggcgggga ggagatcgct
     1981 gccggagaaa ccgcaacctg cggattcatt tttacagccg aagataatat agcgcttaca
     2041 aatttaacag tgaggtaaat ttttatgcat agcttagaga aaaagattat ttcaatcatt
     2101 ctgaccgtta tcatttctgt ctcatcctgt gcggttaccg cagttgccgt tgacggggat
     2161 atcataactc aagtcagcag cgttgagagc gcaactgatg tattgctcaa cagcaagcac
     2221 gttgatgagc ttgaaaagga cgaggtttat ccgacgatcc ttattcacgg aatcggtcag
     2281 gcaaagacct ttatgcttga cggggaaggc aacgatgctg tagatcccga cggcaaaaaa
     2341 attaccggtt ggcctcttta tttctacgtt cccgagcttg ttatcaagct tatcgttccg
     2401 attattcttt ctcttataac tcagaaggac tgcggccttt caaaaacagc ctataatgcc
     2461 gtttacgatg cactggaata tatcgcatat aacgaggacg gcactccgaa gaatgatttc
     2521 cgtgttgaga attacggcaa tcgttctgtt gccgagtgta cggaggaaga aaaagaaacg
     2581 atttacgacc atgttcctat taagggctat accgacgtag tcggcgagga gaatctctat
     2641 tattttgcat acaattcatt cggcgatatg tatgagattg tcgataatct tgaaaagctt
     2701 attgaaaaag caaagaagga tacaggcaag gacaaggtca accttctccc gatcagcctc
     2761 ggcggtgcgg tttccgttgc atatgtcggc gagcatccgc agggtgagga tatcaacaag
     2821 atcgtccttg tcgttcctgc cgcagacggc tctgaaattg tcggcaaaat catgctcggt
     2881 cagcttgatt acagcgacga aggactttac agaaatatgt tcacaaagct ctgcggcgtg
     2941 gattcctaca cgggttggct gatcaatatt gccatcagag ttttaccgaa aaatgttgta
     3001 atcggattta ttgacggagt agcgtcgggt cttgccgact cggctttcgc aagagttacg
     3061 acaatgtggg gtcttgtccc gtcgtcaatg tatgacgagc ttgccgcaaa gtatcttgtt
     3121 aaaggaacaa agtttgctaa cgacgttgaa aggttccaca atgcacaggt caattacata
     3181 aagaatctca aaaactatgc cgcaaacgga gttaaaatat atgacatcag cggctacgga
     3241 cttgagcttt acagccttat tgattccgat tcaaattcgg acaagataat tcattccgca
     3301 tcaacctnnn nnnnnnnaac ctcactcggc gcaacgttca gcaaggtcaa cgaaaagttc
     3361 ccgaagggtt acacacaaaa agagtatcag ggcacaaact ttatgtcgcc ggacggccag
     3421 gttgacgctt caacgtgtgc attcccgtac acgacatggt ttttcggcaa tcaggatcac
     3481 gagaagcttg cgagaaacga tgttgtaatt tcgcttgcca tggagctgct gatcgttaag
     3541 gatatggacg ttttcacaac ggttgaatat cctcagttca acggacacag aaacaccaga
     3601 gaccttaaaa aatacattgc cgaggcggaa aaggttgacc tttcaacgct cnnnnnnnnn
     3661 ncacccccga ggaggcggag agactcacaa atgcacttgc cgacgcaaag gcgatacttg
     3721 ccacaacgat agttgttgag ggcgaggagg aggccgctga ggaggagctt tacaacgctc
     3781 ttgttgagat tggtgtctat gaaaagaaag atacaaccaa ggacgagttt cttctctttg
     3841 cttgcaaaac aacaagcgaa ttgatttatt acctgttcgg gccgagaggc ttcggcgagg
     3901 caaaacccgc ataaaccgca catataattt atccccgaca ttcaattgag tgtcggggtt
     3961 atttgtgccg gttaagcaaa agagctgccg caaaacgaca gctccgattt ttatttcagc
     4021 atttccttaa tcttatccgc cgcatcataa agctctttga tttcggcaag ctcaactgca
     4081 cccttatcga ccagggaaga aatttcgctc ttaatcggaa gctttgcaag tacggggatg
     4141 ttcagctcct tggcgacctc atccagcttg ctctcgccga aaacggaaat cttctttccg
     4201 cagtcggggc actcgaggta gctcatattc tcaacgattc cgagaatcgg aatattcatc
     4261 atattagcca tgttgacggc tttcttaaca atcattgtaa caagatcctg cggcgttgta
     4321 acgataacga ttccgtcaac tggaagcgac tggaacaccg tcagcgcaac atctcccgtt
     4381 cccggaggca tatcgacgaa catataatca acatcacccc aaaagacctc gctccagaac
     4441 tgctggataa caccggaaat aacaggtcct ctccaaacga ccggctgatc ctctttatca
     4501 agaagcaggt tgattgacat gacctcgatt cctgtctttg tctttgccgg aataagtccc
     4561 atttcattct gcattgcagg tcctttcaga ccgaatgcct taggaatcga cggacccgtt
     4621 atgtccgcat caagaattcc gcaggcgttg cccttgacct gcattgctgt tgtgagcatc
     4681 gaggtaacaa gcgactttcc tactccgccc ttgccgctga caactccgat aacctttttt
     4741 acgtcgctgt attcgttgca aggaacatga agatcctttt tttcacagct tgaagaacag
     4801 cttgagcaat ctccgctgca tcccatttaa aacatctcct taaaacaata tcttttccaa
     4861 agccagtgcg ctgtcttcaa caagctgaac gcaggggcgt tttttgtaat agtgttcgtt
     4921 acgctcatcg ggtttcggct gagagttttc accctttgaa agtccgagaa gctcacggca
     4981 aacgattgag ggattaacct ttttgaattc tgcgataaac tcctgaactt tatagtagca
     5041 gagctttttg ccctccgtgt catcgccgtc cgttgcgccg tatttcatgc cgatgagcat
     5101 tgccgccgca gaaaccgttc cgcaaacctc acgggagcga cccactccgc cgccgagacc
     5161 tgccgacacc ttcagcgcgg tatcctcatc aaagccgaac aggtcggcat atgcggcaaa
     5221 aaccgcttga gagcaattgt agccgctttt gaaaagctca acagcctttt cacttctttc
     5281 agacattaca aacacttcct ttccatccat tatagcccga gtgtaacagt tatgtcaaga
     5341 gtgatttcct tgcttgacac tttggtttaa aggtattata attaccctat attgttgaat
     5401 cgaggattat attatgaaaa acacttacga gaccccgctt aattcacgtt acgcaagcaa
     5461 ggagatgcaa tatctctttt cacccgattt caagttcaaa acatggcgca gactgtggat
     5521 cgcacttgca gagagcgaaa tggagcttgg actgaatata actcaagagc agattgatga
     5581 gctgaaggct catgccgagg atatcaacta tgacgttgcg accgagagag aaaagctcgt
     5641 ccgtcacgac gttatgtccc atgtttatgc ttacggcgtt cagtgtccta aggcaaaggg
     5701 aattatccac ctcggtgcaa cctcctgcta tgtaggtgac aacaccgata taattactat
     5761 gaccgaagcg atgaagctta tccgcaagaa gctcgttaat cttatggaca agctttcgaa
     5821 atttgcgctg gaatataaag atcttccgtg tctcggcttt actcattttc agccggctca
     5881 gccgacaacg ctcggtaaaa gagcgtctct ttggctcaat gatcttctga ttgactacga
     5941 acagcttgaa ttccaaatct ctcagatgaa gctgctcggc tcaaagggca caacaggcac
     6001 tcaggcgagc ttcatggatc tttttgaggg cgataccgac aaggtaaagg ctcttgacag
     6061 aaaaatcgca gataaaatgg gatttaagga tttttatccg gtttcgggac aaacttattc
     6121 aagaaaagtg gacactcagg tgcttttcac tctttcaaac attgcccagt cggcggcaaa
     6181 attcacaaac gacctccgac ttcttcagca tcttaaagag gttgaggagc cttttgagaa
     6241 aaatcagatt ggttcttcgg cgatggcata caagcgcaac ccgatgagaa gtgaaagaat
     6301 ttcttcactt gcacgttacg tcatggctga tgttatgaac ccaactataa cggcggcgac
     6361 tcagtggttt gagcgcacac ttgacgattc cgcaaacaag agaatcagcg ttgccgaggc
     6421 tttccttgca accgatgcca ttctcgagat ttatatcaac gtaaccgacg gtcttgttgt
     6481 ttatccaaag atgatcgaaa agcatctcat ggcggagctt ccgtttatgn nnnnnnnnna
     6541 aaacgatctt cttgaaagaa tcgcaaacga ccccgccttt ggcgttacac tcgaacagct
     6601 caaggcaatc atgaagcccg agctttacgt cggcagagcg cctcagcaga ccgaggagtt
     6661 tgttgaaaac ttcattaagc cgataactga gaaatacaag gaccttctcg gaatgaatgt
     6721 tgaaataaag gtgtaaaata tatgaaattg gtaaatataa cttgtccaaa ttgtggagct
     6781 aatttaaagg ttgaatttga aaaaaaacaa gcttattgcg agttctgtgg agcagaacta
     6841 attcttgatg atgaaattaa gcatattcaa attgataatg ccgagaaagc tggatacgat
     6901 tttgaaaaag gacgacaaag agcaaaaaaa gagaggcagc aggaaaagag ttcggacaat
     6961 tatattgttg ctataatcgc aaagcacatt aaaaacgaaa agatagtcaa tctctgccgc
     7021 aaggttctca accgtgaggt ttttatgtac cttgttttcg gcgtgctgac caccgttgtt
     7081 tcctgggcaa gctatgcact gtttgacaga attgtaggca agttcattga aaacaacgat
     7141 atccttcaag cttcgatagc aaatgttctt tcgtggatcg cggcagttct ctttgctttc
     7201 gtcaccaaca agctttgggt gttcgacagc aaaagctgga aaggcagtat tgtgattaaa
     7261 gagcttggct cattcatcgc cgcaaggctt gcaacgggct gtatcgaatg gttcggcgtt
     7321 ccggggctta tgaagctcgg tatggatcag gagcttttcg gaacaaaggg aatgcttgca
     7381 aaaatcattg ccagtgtgct cgttgttata atcaattatt tcttcagcaa gctccttatt
     7441 tttaagaaaa aggaatcata aatcatggaa aaagtcagca taattattcc cgtttataac
     7501 gttgaaaaat atctgccgaa gtgccttgaa agcgtactcg gtcagaccta tgccgacctt
     7561 gaaataatat gcgttgacga cggtactccc gacaggagtg ccgccgttat tttgtcttac
     7621 gccgaaaatg acaggagaat aaatctgatt tctcaggaaa atcaaggact ttcgggtgcg
     7681 agaaacacgg gaatcagatc ggcaaccgga aaatacataa ttttccttga cggcgacgat
     7741 tggattgaac ccgaaacggt tgaaacggcg gtcggaatgg cggagaaaaa caacgccgat
     7801 gttgttatgt ggggatatgt tcgagaattt cccgaccgtt cggacgagaa gaaaatattt
     7861 gcttcagaca aaatatttga tgaaagcgga tgctgggatc tgcacagacg aatggcagga
     7921 ctttacggag aggagacagg cgacccgagc aacgcggatt cgctcgtgac ggcatggggc
     7981 aagctttaca gagccgatat tattaagcaa aacggtcttg aatttgttga tacaaaactg
     8041 ataggtaccg aggacgctct tttcaatttg caatgcttcg gctttgttcg ccgtgcggca
     8101 tttatcaacc gtccgtttaa tcattatcgc aaggataatg atgtctctct gacgaggagc
     8161 tataaggccg ggcttttctc acagtggaat gagctttatg accgaatgga gggctatata
     8221 aaagacaata atttacccga ggatttttcg tcggcactgg gaaatcgaat ttgcttcagc
     8281 atgataggtc tcggcttgac cgaaatgttg aatcccggcg ggcacacggt gagaattaaa
     8341 aatatcaaga aataactttc gacgccgaga tacataaatg cataccaaaa gctcgatatg
     8401 cactatctcc ctgctcattg gaggctgttt tttacgcttt gcaagcgaaa gatggctgtt
     8461 tcggtgtata ttttgctgat tgcaatgaaa aaaatcatcg aaaaatgaaa aattttatat
     8521 tttagcgctt taaagtgttg acaagtatga ttttgtggta tataataggc tcatcatttg
     8581 aaaggagagg cagttatgac gcataaccct tactattatt actatatttg tgactgtctg
     8641 ttcagccttt caaagcgata aattcgtttg aaaaacagac ggtcttgatc agttgcggtc
     8701 aggaccgttt attttttgag gagggtattt tatggcaatt aaaattatct tacttgttgt
     8761 atttttcgct gttatgatcg gcgtcggcat ctactgccga aaaagtgcaa cagacgttaa
     8821 cggctttgtc ctcggcggca gaagcgtcgg cccgtggttg accgccttcg cttacggcac
     8881 ctcatatttt tcggcggtta tcttcgtcgg ctatgccgga cagttcggct ggaaattcgg
     8941 cgttgcttcg acatggatcg gtcttggtaa cgcattcatc ggttcgcttc tcgcttgggt
     9001 aatactcggc agaagaacaa gagttatgac tcagcatctc aactcggcaa ctatgccgca
     9061 gttcttcggt tcacgcttcg acagcaacgc tctcaagctt gcaacctcgc tgataatctt
     9121 tgttttcctt atcccataca ccgcttcgct ttacaacgga ctttcacgtt tgtttgagat
     9181 ggcgttcaat gttgattaca ccgtatgtat tgccgttatg gcgcttctga cggcggttta
     9241 tgttatcgcc ggcggatata tggcaacggc aatcaacgat tttattcagg gtatagttat
     9301 gctcgtcgga atagttgcaa ttatttattc cgtgctcaaa attcatggcg gtttcagcgg
     9361 ctctcttgct gcgcttgccg aggtcagcga cnnnnnnnnn nctgtttgca atcattgttg
     9421 ccggcggctg ctatttcctc ggcggattcg gaaggatatt caatgttgat gttgcttcaa
     9481 agggctatga tgaaattatt ccgacaatgc tttcaaatct ttcaccgctt attatcggaa
     9541 ttacgcttat tcttgttctt tccgcttcga tgtcaacgct ttcgtcgctt gttcttacct
     9601 caagctcaac gctgacgctt gactttatcg ttccgctctc aaagaaaaaa gacgagaaaa
     9661 agacgcttat tataattcgt gcttttattc ttgttttcat tatcatttcc gcggttattg
     9721 ccgtaattca atataaaaat aagataactt tcattgctca gctcatggga ctttcttggg
     9781 gcgcactcgc aggctcgttc cttgcgccgt tcctgtacag cctgtattgg aaaaaggtga
     9841 caaaaagcgc ctgtgcagtc agctttatct ggggttcggg catcatgatt gcaaatatgc
     9901 tctttggctc tcatttcccg acaattattc aaagccccgt taactgcggc gcatttgcaa
     9961 tgcttgcttc gcttgttatc gttcctgttg tcagcttaat cacaaagaag cccgagaagg
    10021 aattcgttga tgatatcttc tcatgctatg acagaaaggt taccgttccc gtaagcgaag
    10081 tgcttgagga aaaatgataa gataataata taaatcacag cagagtatac ctgtttatga
    10141 acgggtatga ag
//
LOCUS       DAWBSW010000059         9937 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1442_length_9937_cov_2.529852, whole genome shotgun sequence.
ACCESSION   DAWBSW010000059 DAWBSW010000000
VERSION     DAWBSW010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_9937_cov_2.529852"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5225..5234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7851..7860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2615 a         2400 c         2134 g         2768 t
ORIGIN      
        1 ataatacttg acaatttcgc ccttaaccgt cgggatgttt tctgcctgct cgagtccgac
       61 agttattctc aaaattttaa gcgcatcggc tgaattgatt ttcccgtcgc cgtttgcatc
      121 ggcattggct ttgttgaagc ttttaacatt cattccgacg ctgtattgaa gcacgcaaag
      181 cgcatcggcg gaattgattt ttccgtcgtc atttgcatct ccgagcaaag ctgccgacga
      241 accgacggtc agcgccgccg caaatgtaag tgccgtaaga attgaaataa attttttcat
      301 tctatcatct ccttaagtaa attgttcgac cgtatcacga attgtatcga gaaacgcatc
      361 ctcaccccat gtgttgattt tgaaaaagac tgcctgactt ccgcccgagc ttattgccgt
      421 gagatgcacg tcttctccgc acgcaaaaag tgtgacgctc aggctgactc tgttgccgcc
      481 tatgtagctg taacgctcat agacctttac agagcaggca caattccctt tttcgcagta
      541 cgactcgtcc tcaacggaag cagaaacgct tttgttgacc gctctgtcaa tggcttcact
      601 gatttcaaac aatgaaccgt gaaggtcaca ctcaagcatt gccataaatt ttctcctttt
      661 tactctcaat cttctttatc aagctcctgc cgatatcatg caaaagctta tatgctgccg
      721 ggaagacctc ttcggggaaa ttaacaagaa aatcatcaga ttcaaatgta aaatcgccct
      781 tataatcaat ctcggcaaga gcctgagtga tttcctccca gtcgagatca aagaaatacg
      841 gcggacaatg tctgtcctca agatagtcat tgtcatggac gtgcagacag ccgacattct
      901 cgtgaccgag agttctgagc atttctggag ccgttgttcc gacaaggcct gcgtgtccta
      961 tatcgacgca agcggtaaaa tatctcgggt caagcatatc catcatggct ttgaactcct
     1021 cgccgacgct gcaaatattc ggaataatac aatgtctgcg gcgatccctg ccgaacatat
     1081 tctcacatgc gatttttata ttatattcct tgcaaagcgg aataagatta ttgtaaaact
     1141 cggcattaag ctcaagattt ctcttgctgt ccttgccgta atatgtcgga tgaattacaa
     1201 tatttttcgc tccgagagta cctgcgattt ctattgaacg tttgattttt tcaagaatta
     1261 ccgtgttgta tgtatcctcc ccgaccttaa agctcggaaa aggtgcatga gcctgattaa
     1321 agtatttacc gtagctttca acgattttct ttacttcgat cacatgctcg agatatacgg
     1381 gctgattaag gatattatcg tctgcctgca tacagaacat tgaatagtcc aatgcatcaa
     1441 agcctgcctc gcatatcatg cgaacacatt tttcgtcacc taagcgtttt gagcccactt
     1501 cagtctgagt tgataatacc attttaatca agtcctttcg agtaatagtt gtttaaagaa
     1561 gcgacgttta tatccgatga agctctacgc cctcattcgg cttcactgac agcttttcta
     1621 cgggcgcctt tgtgtaaatt acacttatcc gccttaattc ctcttatcga gaatgcaaaa
     1681 gaataattgc cgctgtgcat cttgtacggc tcatgtaaac acgccgtgcc gggcatatcg
     1741 ccgccgacgc ctcgttgttt cgcatcaatg caaagcgaat aatattcatc cttggtgagc
     1801 tcgtgattat gcattgcttt ctcaattttt tcctgagaat agctgtgcat ggagaactca
     1861 aacgggaagt ccgagctgac ggagaagccg ttgccgctgt catcagtaat ttcaagcgaa
     1921 cgcacatcgg ttcggttgcc gttttcctgc ggtctcatat aataatggaa gagttcatcg
     1981 gccttcttat tgaattcaga aatgcgctgt cccgtttttc tgtcacaata cgactcctcg
     2041 ggacctcttc cgtaccactt gacattgcaa aaatccttgt ttattccgag tcttacaccc
     2101 gaacgcagca tcggaagtaa gggactgttc atattttgtc cgatgcttac acttccgtcg
     2161 ctgttgacga ttatatcagt ctcaacatga cctgcaaacg gtgcaaacca atgaatattt
     2221 actatacatg atccgctgaa cgggaagata acaaagctct ttatgctgac gcttcggctt
     2281 gttatatccc aaaggtagag cggattgagg cgtttgaaca tcggggcaaa attaaaatag
     2341 tttgtgtcgt tatcagtcaa cgggcggaag aaattcggct taaatgaaag cgaattgtca
     2401 agaatgtttc cgctgccgaa gtcgattccg taaagctttt tatttttaaa ggtaacggtt
     2461 gtgccgcttg caattatctt gtggacgttg ccctttgaaa gaaccttgac catgccctct
     2521 ctcggctcgg gcttcgcctt ttcgttgctg agaatgaact gattgaagct gatttcatca
     2581 ttcgccctgt gccactcatt ttcgtcttta tacctgaacg aaagtgtaag aatatactca
     2641 cctgcttcaa actcggcaga atacgggatt gagagcgtct ttaccgtctg cggagcaatt
     2701 ccgtcgagtg agattctgcc gctttcaagc tccttgccgt tctttgcaac ggaccattcg
     2761 agatagcaat aatcaagcgg aataaaataa ttcttattct ttatcttaac tctttttctt
     2821 tcaacgtcga tatcctcggc gcagacattg gaatagacct gcttgacctc ataatatgcc
     2881 gggtgtggcg tgcggtcggc ggcaattatt ccgttggcgc agaaatagaa gcttgaccaa
     2941 ccttccttaa agtcgccgcc gtatagccac ttaccgtcct tgtgtatcgc ctgatcaacg
     3001 aaatcccata taaatccgcc cgtcatgtgg tcgtactttt caaaatcatc aacatattcc
     3061 ttgaaattgc cgaggctgtt ctccattgag tgagcgtact cacaataaat caccggatga
     3121 tcctcaaatg ccgattccgg tacgtccttg ttgtcggcgg caagggcatt ggctatgttg
     3181 tcaaacagtg tcgccgtgat tgcttcccga tgaacgagct tgtcaacaac attttccatc
     3241 gggtacattc ggctgataaa atcagacttt gtgaggtcaa aatcgccctc atagtgtatc
     3301 ggataaaggt cacaaagctt caaaatcgcc tttttcatct cggcaaaatt cgatccgtct
     3361 cccgcttcgt tgccgagcga ccagaagcag atacaggcgt ggcttctgtc acgcaaaacc
     3421 atcatctctg ccctgtcaat tactgccgag gtccaaagcg gattgtcgcc gggtacattt
     3481 tttctgcgta caccgtgact ttcaacgtcg cactcgtcca ttacataaaa gccaagctcg
     3541 tcacagagct catagaagaa cggatcgtcc ggataatgac tggttcttat ggcattgatg
     3601 tttgcctttt tcattaggta aagatcctga taatatctct ccctcggcac agcccagccg
     3661 taatcggggt catagtcgtg acggtttacg cccttgatta taaccttttg tccgttaata
     3721 tagtaaacat ttccttttat ttcaacacgc ttgaagccta ttcttatctc ttttttgacc
     3781 gagcagtacg tgccctcata gagcttgacg gtcaaagtat aaagctcggg tgtttccgcg
     3841 ctccatttgc ggcatttttc gacaatgcgg tttatttcaa gctcgccgct tacctttccc
     3901 ctgaaaatct cctttttgtc aaggtcaaca cggcatgtaa cttccctatc cgtgctgaat
     3961 ttaagtttca ggtcaagtat gccgtcaatg tagctgtcat caagtgttgt tcttgcatag
     4021 atatcctcaa ccgtggtttc gggctcggca acaaggtaga cctcacggta tattccggag
     4081 aactgccaca tatcctggtt ttcaagatag gttgcgtcac tgaagcgcat aagctttgcg
     4141 gcaagagtgt tttcgccctc atgaagaata tcggttatat caaactcggc cggggtcatc
     4201 gagccctgcg aataaccgac atattcgccg ttgatataga cataaagcgc agactttgcc
     4261 gcgccgaaat gcagaatgat cctcctgccg tcaaagcctt cgggcagagt gaagctgcgg
     4321 cgatagaaac ctatctcgtt cttttcgtgg cttattttcg gaatctgcga ttcggcagtc
     4381 gatacctgcg gcggcattga cgagcagaga taaatcggct tgccgtagcc ttgcatctgc
     4441 cagaccgacg gaacatttac cgtatcccag ccgctgtcat caaaatcggg acagaaaaaa
     4501 tcctgcggct gattttctac ccccatctgc cacataaatt tccattttcc gttcagcgaa
     4561 agcttgtatt ttgacttgcc gtattcaacc ctgtctcttg cgtcatacgg caaagcgagg
     4621 ttatgcccgt cttttttgtt ctcacgaaag aataacgggt tttcccattt gtgaagcggt
     4681 tttttcaaaa aatcaactcc tgtctttaac cttcggttac tataattata tctatagttt
     4741 gtatgcaagt caaataaaaa ttagtttatc tgtttctttt ttatacttta tatgcaataa
     4801 ggcaaaaaat acagggctcg taaaacaaaa tcagcaaatc ttattgaaaa aaaaagccgt
     4861 tagtattata ataaatcgaa tgttgcaatt tttgaggtgt tggtgttgaa aaaaatcgga
     4921 tttgataacg aaaaatatgt taagatgcaa tctgagcata taagagaaag aattaacaag
     4981 ttcggcggca agctctatct tgaattcggc ggcaagctct tcgacgatta ccatgcatcg
     5041 agagtcctcc cgggctttgc acccgacagc aaggttaaaa tgcttatgaa gctcaaggat
     5101 caggcggaga tcgttatcgt cgtcaacgct tccgacattg agaagaacaa gataagaggc
     5161 gaccttggca ttacatacga tcttgacgtt ctcaggctta ttgacgcttt cagaggaatc
     5221 ggacnnnnnn nnnntaccaa ccttgtactt gaaagcctcg gcgttaaggt ataccgccat
     5281 tacacgattc ccggctatcc gtccgacgtt gacagaatcg tcagcgacga gggattcgga
     5341 aaaaacgaat atatcgaaac ttcccgttca ctcgttgtta tcaccgctcc cggcccgggc
     5401 agcggaaaaa tggcgacctg cctttcacag ctctatcacg agcacaaaag aggaatcagt
     5461 gcaggctatg caaaatttga aacattcccg atttggaatc ttccgctcaa ccacccggtt
     5521 aaccttgctt atgaagcggc aacagcagac ctcaacgata taaacatgat cgacccgttc
     5581 caccttgaag catacggcga aatgacagtc aactacaacc gtgacgttga aattttcccg
     5641 gttcttgcgg caacctttga tcagatctac ggcgaatgtc cgtataagtc acccacagac
     5701 atgggagtta acatggcggg caactgcatc attgacgatg aggcatgctg cgaagcttca
     5761 agacaggaga ttatcagacg tttctatgcc gaacagtgct cggttaaaaa gggtctcggc
     5821 aattcgggaa gcgttgacaa aatcaagctt atcatgaaaa aaacgggtat ttctcttgat
     5881 gagcgtcccg ttatcgccgc agcaaaagcc aaggcagagg aaacaggaga gccggcaatg
     5941 gcaattgaga cgggcaagga tactcttgtt acgggcaaaa cctcacttct catgggtgca
     6001 ggtgcagcgg caatgcttaa cgcacttaaa agccttgcgg gtatcaacga cgatattcat
     6061 ctcctctcac ctgccgttat cgagccggtt caggagctga aagtaaaata tatgggcaac
     6121 cacaacccga gacttcacgt tgacgaagtg ctcattgccc tctccgtttc tgcggcaaca
     6181 aatccgcttg cgaagcttgc acttcagcaa attccgaagc tcagaggtat ggaggctcac
     6241 gcaacggtta tcctcaagga gcaggacgaa tccgttttca aaaaattcgg tatcaacata
     6301 acctgcgagc cgaaatatca gaccaaaaag ctctatcaca aatgaggaga acagcatggg
     6361 aaaaattgtt gcaatcggcg gcggacgcta cagtgacggc gaaataatca atatctttga
     6421 aaaaatagtc tcccttgcgg acaaacagaa ccccaaggtt ctgtttcttc cgacggcagg
     6481 ctatgacgat atcaacggag acgaggatat tcagcgcagc tttgaaaaat acggctgtca
     6541 atataatatt ctctatctca cggatgagaa aagaaccgtc gatgatattc gctcagtcat
     6601 tctcgatgcg gatatcgtct atgtcggcgg aggtgatgtc cgcttcatga tggaaacctg
     6661 gcgcaaaaca ggggccgata aagctcttat tgaagcatat aacagcggca aaatcatgtc
     6721 aggctacagc gcaggcgcag tctgctggtt cgatgtcggc tatgacgact gcggcccgga
     6781 gcacagcttc atgttctgcg actgcctcgg aattctcccc tactgtgcct gtccgcacta
     6841 tgaatcggac cactggcaga ccttcaaaaa ggctattctt gaccgacccg agcagggact
     6901 tgcgattgaa aacggcgccg cattgctttt cgtcgacggt atgataggta cggtcagggg
     6961 caatgacggc ggcgaggtat ggattcttga caaaaataat gaactttgtc tcagcaaaat
     7021 tgtcgataaa ataaacagaa tcgactaatc gtcggttgac tttacagtta gtttgtgcta
     7081 acatatatac gaaaaaaata atggagggtg aaaccatgaa aaaagtatta tcaatctttt
     7141 tagctgttgt tattctcatt tcgggcgttg tttttgcgtc tgcaaagggt aatgacaagc
     7201 ttcagtttga cgagaacgga cagtttaaga ttcttgctct tgccgatatt cagtccggct
     7261 tcccggtcgg cgaagcactc aaggcttata tagctgaagc tattgacgcc tcggatcccg
     7321 accttgttat gttcctcggc gataacatta tgagccccga ggacggcacg gttgaaagct
     7381 actggaaagg ctacgacgag gtattcccga tcctcgaaag caagggcgtc ccgttcactt
     7441 tcgtattcgg caatcacgat gatgagtccg ctccgacggt aacaaaggaa gaaatgctca
     7501 aaaagtatca gtcctatgac ggctgccttg cttatgatgc agacccgtcg cttcacggct
     7561 gtgcaactca caatcttccg attctctcaa gcgacggcaa aaaggtcgct tacaaccttt
     7621 ggatgttcga cagcggagat tatgttttct acgagaacgg caaaagaaga ggctatgatt
     7681 gcgttcgcgc agaccagatt gaatggtaca aggcagagag tgcaaagctc aaggcggcca
     7741 acggcggcga agctgttccg tcacttgcat tccagcacat cattccgcag gaagcagctc
     7801 aggctgttat gttctctctt ccgttccagc tcggcaagct cactaagaac nnnnnnnnnn
     7861 ccgaactact ttgcgttcaa cggaatcctt tccgaagctc cgtgcccgtc gcccgataac
     7921 gagggtcagt gggatgcgtt cgtagaaaca ggcgacgtta aggcttgctt cttcggccat
     7981 gaccatgtca acaacttcaa cgttgatgtt gacggcgtta cggcagtcag cgttccgggc
     8041 acaaccttca agtcatacag cagcgttacg gatcagggat caatggtaat tactcttgac
     8101 gaaaaagatc tttcaaccta cagcacagag attctttaca caagcgatct tgcggttaag
     8161 gacggctcaa acatcccgaa tcaggagcac tcggagactg ttgcaggata caggttcaga
     8221 atagtacttc gtcatcttgc tcacggaatc cttgcacttt tgagaggaat ctatgcttgg
     8281 atcccctcac ctctcggcaa gtaatcttga ccgaaaagta aatatttaag gcaacgagat
     8341 caaaacgatt tcgttgcctt ttttacttat aattatttac caattcgtga ctggattgat
     8401 atacagcttt cttaatatcc gatttttgtt ttatttatcc tttgcatcct tattcttggc
     8461 aaatatcata acaagtcctg cgattccgct gattataccg accaaagaga acggaaaatc
     8521 gtaaaaaccg gttaaaagag tattgttaat atccgcaccg gctacacaaa gaagtatacc
     8581 gaacaaaatc aaagcgattc cttttgcaat tttattattc atagcgtttt ctcccaaatt
     8641 atacaatctt ttcaagtccc gatttgacgg gcttggttac atagaccttt ttaacgattg
     8701 atttcaggct ttctgcggcg cactcggctt tcgactcgtc gtcaaagatt ccgtaaaccg
     8761 tcggaccgct tccgctcatg caagccgcca acgccccgta gcgattaaga accgatttta
     8821 tcggcacacg ctcgggcacc tcgataagct gctcaaaaat atttttacag cggcggcaca
     8881 tctcataaag atcgccctgc gatgcggcat acagcattcc gcaggtgtcg aggtggcgta
     8941 tattctgcgc cgtatcaaaa gcagagtaag cctcttttgt tgagactccc ctctcgggct
     9001 tcgcaagcac tatccggcaa tccgttaaat cgggcagcgg agaaagaatc tcaccgatat
     9061 tctgtgcaac gcatgtgccg cccgtcaggc agaacggaac atcagcaccg accttaacgc
     9121 cgactgcaca aagctccttt aatgtcaaac ctgccgaaaa aatatcgtta agcgcggcaa
     9181 tgaccgccgc tccgtccgca ctgccgcctg ccatacctgc ggcaaacgga atgttctttt
     9241 caatatgaat ttttatacct gtgtttttga tttttgtata gtcaaaaaat gcctgcgccg
     9301 ccttgtatgc aatgttcgtt ttgtcgcacg gcagagccgc ttcgctgcac gttatcgtga
     9361 tctttctgtc gtccgttgtc tcaacactca ccgtgtcgta aagtccgaca gactgcatta
     9421 tcataaacag gctgtgatat ccgttgtcaa gcgttcttag tatatcgagc atgaggttta
     9481 ttttggctgc tgccttgtat tctgctttca tatgttcttt cctttcgggc agtttaagtc
     9541 tgtattgcgg taggcggttc tgattatttc caaatcccca ccaccgcaag cggtccccct
     9601 ccccttgctt caaggggagg ctaaggccga cttcaagttt atagtttttc taaactcttt
     9661 ttaactatat tgtcaatata caggcaaact tcatcaaaaa tactgtttat ttcattgttc
     9721 ggtattctta ttacctgtat attatactct tttaaatatt ctgttcttat atggtcttta
     9781 atcattcccg tatcttcata atgctgtgct ccgtcaagct caataaccaa gttcgctctt
     9841 gaagaataaa aatccgcaat atattttcct aaaatttttt gcctataaaa tctgatagga
     9901 tatccactca aaaagtcata ccacaatttt ctttctt
//
LOCUS       DAWBSW010000060         9879 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1448_length_9879_cov_2.764149, whole genome shotgun sequence.
ACCESSION   DAWBSW010000060 DAWBSW010000000
VERSION     DAWBSW010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1448_length_9879_cov_2.764149"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    793..802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2659 a         2320 c         2229 g         2661 t
ORIGIN      
        1 caacgggctt ttacgttttc tttttcttag ccattcatac cactcctctc agcttaaatc
       61 gagcatgtcg atataaagat gtccgttgtc ggcaatgtgc tccttacgtc ctgcaaggtt
      121 gtccccgagc agcatatcaa atatctgtga cgttctctct gcgtcctcgg gaagaatctc
      181 gacaaggcgg cgagtctcgg gattcattgt cgtcatccac atcatatccg cctcattttc
      241 accgagtccc tttgaacgct gaattgtata tttctgattt cctatctcct caagagcctg
      301 cgctttctct ttttcggtat aggcaaacca tacctgatcc ttcgtgttga tttcgtaaag
      361 cggagtttcc gcaataaaga ccttgccttc ctcgatgagg gtcggcataa gtctgtatat
      421 catcgttaaa ataagcgttc ttatctgaaa accgtcaacg tcggcatctg tacatataat
      481 gaccttattg tatctgagca tatcgagatt gaactgtgca agacccttca tcgctttgct
      541 cttgacctca acgccgcagc cgagtacttt aataagatct gttatgatct cgctcttaaa
      601 aattctgtcg tactcaacct tcagacaatt gagtatctta cctctgacgg gcatgagtgc
      661 ctgaaattca gagtttctcg cctgcttaca ggcaccgaga gcagaatcac cctcaacgat
      721 aaaaagctca cgttcgcttg cgtcctttgt gcggcagtcg acaaacttct gaactctgtt
      781 agcaaggtcg ttnnnnnnnn nnttctcagc ctctgccctg ttctctatga aatagatttc
      841 aagctgatgc ttgaagaaat ccgtcatcgc ttcctgaata aatttatttg taatagcctt
      901 tttcgtctga ttttcgtatg aggtctgggt tgagaacgag gaaataacga gaatcataca
      961 gtcctcgata tcctcaaact tgattttcgg gtcgtttttt acatattttc cgttcgcctt
     1021 gagataagcg tcgatctgat atgtgaacgc atttctgacc gccttttcgg gtgcgccgcc
     1081 gtgctccaac cagctcgaat tgtggtagta ctccttaagc tggtttttat ttgagaagca
     1141 aagggcggaa ttgatgagca ccttgtaatc attgaggtcg tcacggtcac gtccgacacg
     1201 ctcggctttc cacgtctgca cacttgtcat tgcgttatcg ccggcaatct ccttgacgta
     1261 atccgtgatg ccgtccgtgt agcaatattc aaacgtttca aactgagtct tgttgagctg
     1321 atttttgaga ataaacttaa cgcctgcgtt gactatagcc tgacgcttaa tagtgctctg
     1381 ataatattca agcggaatgt cgatatcggt aaagaccttg agatccgggc gccattttat
     1441 gcgtgagccc gtgtccctct tggtgtattt ttccttttcc atttcgccga cggccttgcc
     1501 ttttttgaaa ttgagcttat agcaccagcc gtcacggtga atttcggctt ccatgtactc
     1561 cgaggcgaac tgggtggcac aaagtccgag tccgttaaga ccgagtgagt attcatagct
     1621 gccgccgttg ttcgtgttgt atttgcctcc tgcataaaga gtgcagaaaa caaggaacca
     1681 gttatagttc ttttctttct cgttatagtc aaccggaatt cctcggccga aatcctgaat
     1741 ttcaaccgac ttgtcgagaa agcgcgtgat aatgatttta tcgccgaatc cctcacgagc
     1801 ctcgtcaatt gagtttgaca aaatttcaaa aacggaatgc tgacaacctt ccaatccgtc
     1861 ggagccgaaa ataacggcag gacgtttacg aacctggtcg ggtcccttga gcgccacaat
     1921 atcgttattt ccgtagcttg tcttttttgc cataaaaatg accattcctt tctctaaagc
     1981 aatactttcc aagatacaaa gtatatttta cactatatct tgtggtttag tcaagctttt
     2041 ttgcatatga atattcccgc cctgcacggt gccaagggga ggcttctgcc gaacttgagt
     2101 cgctgcaggc atcggcccct acgaattgtg cttttaccaa acctatacaa cacatctcaa
     2161 cgaaaacaaa aactgccccg aaaatcggag cagtcttttt aaaatttgca gcttatttaa
     2221 ttatctcacc catcatgccg ggagttgcga aagctgcgtt tgactcatct gtatcaatgt
     2281 gcattgcaag ggcatacttg gggcttactc ttacaacaac atcaccgaaa acaagcgaac
     2341 gtccgttgga gttgatctta acggaaacta tctccttatc cttgagaccg aacttttcgg
     2401 catcctcggg agttgcatga atgtgacgct ttgcaacgat aacgccctcg ctgagctcga
     2461 cctcacctgc ggggcctacc agcttacatg caccactgcc tgcgatatcg cccgactcac
     2521 ggcagggtgc gtttacgccg attgaacgtg cgtcggaagc tgaaagctca acctgagtct
     2581 cgggacgaac ggggccgaga attgatacag cggggaagct gctcttggat ccgataacct
     2641 gaaccctttc gtttgaagcg aactgacccg gctgagaaag atccttcttt ttggtaagct
     2701 catatccctt gccgaagaga gtttcaagaa cctcctgtgt gacatgaaca tggcgagccg
     2761 atgtttcgac taaaacctga tttgacattt taaacaacct cacttttatt ggtttccgtc
     2821 aattattata aaaccttatt ataaaaaatg caactgtttt tattatcatc tttactttct
     2881 tatttgttga cgaatgggtt tacagatgat ataatgtaaa tattgcggat atctgagggg
     2941 ggaaatgctt gattacaaag aaaatgagca gcacaataaa atccgtcgga tttattatga
     3001 cccttataat tgttttttat cattgcaatt acagcggtga acccttcggc tcatttgacg
     3061 gcaaagcaat cggtattttc gattcatctg ccgaagcttt ggcttttgtt gctatgtgct
     3121 ggttcttttc aatcagcggg ttcctgcttt ttcgcaatct gactcttaag aattatgcag
     3181 agaagttgaa aaaacgtgcg gtttcgcttt tggtgccgta tttcatctgg gaatttgccg
     3241 cagttgttcc gctgatcgtt ataccgctca tacgtcacag ctcaattgtt tcttccaagc
     3301 tttttcaaga tttgctctgc ggcgtatttc tgatgaaaag cctgccgccg aacggtgcgc
     3361 tttggtatct ctacgcactg tttttgctga ccgctttatc tccgcttttt cttttgctat
     3421 tcaaaaataa aaacatcggc tggctcgcaa ccgtggcttt gaccgtgctt atatattgcc
     3481 tggacaacac cgacaacatc gttagcaggc aaattctttc ctacggatat atcaggctgg
     3541 ttttttcata tctcccgtcg tttctcatcg gagcgtttat gggtcatttc tcggagaatt
     3601 ccgacggaac cgataagata aaatatatcc tgtctctgct tcttgcaggc atactttttg
     3661 aaggggcttt cacgggaatt gcaaaggatt cgcttgtcag aattcttccg gtgctgattt
     3721 tgtattttat gcctgacctt acggcttttg aaggcaaaaa agcttatcga atttcatttc
     3781 tcgtctatgc aattcatcag ccgcttcttg aattcaggcg atttttgatc ccggccgtgt
     3841 caaaaatacc gtatgcgttc tttgcaagcc ttacgcttcg ctttttattc attataatta
     3901 taattgccgc tgcagcgttc attcactttg tgctcggaaa aatcgctccc aagctgttaa
     3961 atatcgttac aggcggaaga tcataaaaaa taaggtgcta aaatgaagaa aaaaacattg
     4021 aattcaatcc cgaaattacc taccgccgtt caagtgacat ggtatgtgtg tcgagggctc
     4081 cttttcatat ggggcgcagt catgctttta accggctaca caacggaatt tcttgaggcg
     4141 gtttttgcga taatttttac ttttctgtgg gatctctttc agcttctcgg aggaaaaact
     4201 tttatcacac tctttccgca ccgtgtgcag acgatgctca atcttttgat atgcttcggc
     4261 gtcgtcgtag gctctaccat caataaattc acaacctttg agcacataga tattcccgag
     4321 catttctttg caggctatat cgccgccgca ggcagctatg agctcgccgt cataatccaa
     4381 agcagacagc caaaggacaa gaagctcagt ccggcgcttg ccgcaatgtt ctgtcttgcg
     4441 ttttccgtaa tgctcctgac aggctgggag ttttacgaat tcacaatgga caggctctac
     4501 ggtcttaatc ttcagcgctc gggcttcaat accgaggcgg gactcatcga cacaatgatc
     4561 gaccttataa taggtgcggc aggctcactc accggaatgt ttatcacggc gttctcaaaa
     4621 gcgggatttt tcaaaaagaa aggcaaaaag aatgaacaga agcacaacta aaataattgc
     4681 tctcgtcctc gcaggactga tgattctcgg aactctgaca ggcatcatta ccacgttgct
     4741 ccattgacat ataacgggca atttgataaa ataaaattat acggacaaag gcaacacagc
     4801 acaaattgtc ttaagaaagg atagtgaacg taatgaaaaa cggcataaaa cgtatcggcg
     4861 cattgcttat gatagttgcg ctgtgcttct ctgttgcgtt tgaagcggcg gcggccggct
     4921 atcccgacta ctatccgaat actcacagaa acaccggttc gcacattgca gacttaatag
     4981 gtgttgcaaa aacccagctt ggttatacgg aacttgacag ctccggtaat ccgatttcat
     5041 ccgcaagcga cggcggctat acaaaatacg gtgcttcctt cggtgagccg aacggcgcat
     5101 ggtgcgctta cttcatatcc tggtgtgctt ctcaggcagg cattccttcc tctgtcgttc
     5161 ctcggctcgg caactgcgta acgcttaccg actggtatca aagcaggtcg atttattatt
     5221 cacgctcctc gggttatatt cccaaaccgg gcgatatggt attctttaat tggagcggcg
     5281 gctcaagtgc acagcacatt ggaatcgtaa ccggcgtttc cggcggcagt ctctatacca
     5341 ttgagggcaa cacggacggc gacgagggct atatgtgcaa cggcagaaca agagagctga
     5401 atgcctccta tgtcctcggc tacggagttc cgaaatataa cgacgcttca acctatgtcg
     5461 gctcatacgc ctttgcggct tccgtttctt cctcgggcgg ctcacattcc aattcaaccg
     5521 catacggaag taatctctcg gttgtaacca ccacggcgac aggtataaca agcagcgacg
     5581 caacgctgaa cggttcgatc aggaatacgg gcggaaacta tgtttcggcg gcaggcttct
     5641 acttcggcgc tgacaaaaat gaccttacaa aatatcccgt tgtttcgtca tcatcaagca
     5701 aggaaattcc gctgagcatg agcgtttcaa aaaaattcgg caagcttaag ccgaaaacca
     5761 catactatta cagagcgtat gccacgataa acggcaatct ttacacgggc ccgatgtatg
     5821 ccgttgtaac ggtcagtgac gttcctcagc agcttgttct cagcgaaacc tcaattaacg
     5881 tgggtatcgg tcagactgcc gaaatcatgt gggcgcagct ccctgtcggc tcgaccgata
     5941 aaggcgtaac atggcaatcc tcggatccga gcattgccgt tatcagcaat acaggaatta
     6001 taaccggcat gggctacggc aaggttactc tcacggcaac gacaaattac ggctcggctt
     6061 tttcctcggt tgacgtcaac gttctgatcg gcaagccaca gaatatcaag ctgacaaata
     6121 ttgccaagga cgaaataggc ataagctggg ataaggttga caaagcaaag ggttacataa
     6181 tttaccgatg cgaaagctca aacggtaaat tcaaggaaca taaaaagctt gacagcagca
     6241 caacaagcta cattgataag gacgttaaag agggtcacag atattattac aagctcaaga
     6301 ccattgctga taaagaaaaa tatgacagcg atattaccga aatcatgtac atcacggcaa
     6361 agctccctgc gccggttgtt tcaaaggtcg aaaatgataa aaacggcttt ggaaatctca
     6421 catggagcaa ggtcgacgga gcggaaaaat atgttgttta ccgttcaaga accgttgacg
     6481 gcgtttatgt tgcaatcagc gacgtaagag gtacgagctt caccgacttc tctgcaaatc
     6541 ggtcgggcga atacttctac aagatagtcg cttacgcagc caacgaaaag gcgacaagct
     6601 cattttccga ggttgctacg gtggcgatca cgggcgcata tgccgacgac aatgaaattg
     6661 aaacggtaag aacgcttgaa cgtcagcggg aaaaacagtt tgacgaagaa aatgttttca
     6721 cgagcaaaac aggtttactt aacataatat aacttatctt atcggcagta cctttttcgg
     6781 tgctgtcgat ttgttttaat tgttgctttt aatgctcgat tattatataa ttatatcatt
     6841 aaagcacgga ggaaaatgga aaatatgctc agacttgtaa caggacggat cggctgcggc
     6901 aaaaccgcac gcatttatga agaaattcgc aaaataaccg aacagggcgg cgaggttaca
     6961 ctcgtcgttc cggagcagta ttccttccat acggagaaaa aaatgcttga aatgctcggt
     7021 gctcggggtg ccgacagggt caatgttgtc agcttctctt tccttgcaaa gaatcttttg
     7081 aagaaatacg gtttgaattc caaaaagtct cttgacgaca gcacccgtgc catgctgatg
     7141 agtgtcgctc tcgacgaagc gggtgacgat ttgcaggtct attcacgcca cagatacagt
     7201 ccggccgtaa ttttgcagat gctcaaaact cttaaagatt tccgtcagtg ctctgtgacc
     7261 tccgataaaa tcgaagagac gctcgcaaag ctgaagccgt cgcttcttaa agataagctc
     7321 ggcgaaattc tgcttatcga cagaacgtac accgcactgg ttgagcagaa ttattttgac
     7381 gacgactgtg cgcttgatat tctttgcgac gttttggacg aacacagaga ttttgacggt
     7441 gcagaggttt tcgttgacgg ctttcgcggc ttcacggcgc aggaatatgc cgtgctcgaa
     7501 agaattatgc gccaatccgc cgaaacttgg gtgacgcttt gcgttgacaa agcagatgat
     7561 gaattcggcg tttttgccca cacgttcaga acaaagcgca agctcatccg ctgtgcacaa
     7621 aagagcggaa cagatattgc gaagcctgag cttctcaccc gtggcggcag cattgacccc
     7681 gagcttaatg cccttgaaaa aggtctctac tcgggcggtg ccgaaatata cggtgaagaa
     7741 acgaaaataa tcactctttg ttcggcggaa gattttgaaa gcgaatgtga gttcgtcgct
     7801 tgcacgatca aaagactcat cagaaccgaa aacattcgtt gccgtgacat tgccgtaatt
     7861 tcgcgaagcg aaaataatta ttcaaggcag attcgctctt cccttatcaa gaacggcgtt
     7921 cccgttttcg aggacaagcg tcaaccgata accactgagc cgctgattga atgtgtcagg
     7981 agtgcgattg aaataggtac atcgggcttt tcgacagacg cgcttatgcg acttgcaaaa
     8041 acaggactta ccgaccttac aatcgaagaa atcgcaaaaa tcgaaaacta tgcgcttatg
     8101 tggcagataa acggtaaaaa atggcttgat gaatggactg ctcacccaaa cggcttgggt
     8161 gaaaaaatgc ttgaaaagga tgccgccgag ctcagggaaa taaatgacac aagaataaag
     8221 ctcgttgcac cgttgcagaa attcagagtc aagctcaggg acttcacgtc acttgaagcg
     8281 gcgcaggcta tctattacct gctcattgac atgaatgttg gcgaaaatct caaaaagctt
     8341 gcgataaagc ttaatgagag ttcacagtcc gagctcgcct ccgagcagga aagaatatgg
     8401 tcgctgacaa tggaaatgct cgaccgtata gccgactctc ttgaaggagt gcgactcacg
     8461 ccgtcaagat tcagagagct ttttgatttg ctggtttcca cctattccgt aggcaacctg
     8521 ccgcagggtc ttgacgaaat cactatcggc agtgcggaca gggttaagac ctctgcgccg
     8581 aaaatcgtat ttgccgtcgg aatgaacgac ggcgttttcc cgatgattcc gaattccgac
     8641 ggcatttttt cggagaacga gcggcgtgag cttgccgaat ttgaactgaa aaccgacgac
     8701 agctttgaag aaaaaatgat ggaggagcgt tttatagcat acaatacgct ttgctcaacc
     8761 tccgacagac tttacgtcac atatcttcgc aaggacataa acggcgcaca ggtttcccct
     8821 tcggaaatcg tcactcagat aaaaaagctt tttccgaagc tcaccgttat tgacaccgtt
     8881 gatattgacg acaccgacag gatcgaaagc gtcgaaaccg ccttcgatat catggcggag
     8941 ctaacgcaaa aaaacaccgt tctgcacagc acgcttgaaa aatatttcga agctgttccc
     9001 ggatattccg accgtcttgc cgccctcaaa agaggaatta acaaagaaga aatcaaaatc
     9061 agagacaagg aaatcgcaac caagcttttc gggctcaaca tgtatatgtc agcctcgaga
     9121 accgaggttt atcacaagtg tccgtttgaa tatttctgca aatacggact gaacgctcag
     9181 ccgagaaaga ccgctgagct tgatccgatg caaaagggca cagcgataca ctatatactt
     9241 gaaaagctga tttccgcata cggcagtgac ggactttgct ccatggagaa agcagaccgt
     9301 gacaaatgcg ttttcgacat tcttgaggaa tatttcaaag aaaatctgac atccggcgaa
     9361 gaaatgggtg aaagattcga ttacctcttc cgtcagctcg gtctcaccgt ctgtgaggtc
     9421 gttgacaggc ttgttgcaga atttgccaac agcaactttg ttcctgtcgc atttgagctg
     9481 agcattgaca gcgacggcga ggttgatacc tataacattc ccctgcccga cggcggaacg
     9541 cttaaaatca aaggctcggt tgaccgtgtt gacgttgccg aacagaacgg aacgagctat
     9601 gtcagggttg tcgattacaa atccggagga aaaaaattcg acctcaacga ggttttctac
     9661 gggttgaata tgcaaatgct tatatatctt ttcgccatat ggaaaaacgg attcagggat
     9721 tacaaaaata ttacccctgc cggtattctg tatatgcccg ttaatgctcc cttcgttgaa
     9781 acggagcgcg acgagaacga aaaaacgatt gagcagaaaa aactcaaagg aacaaaaatg
     9841 aacggcatgg tgcttgacga cagccgtgtg atttacatg
//
LOCUS       DAWBSW010000061         9791 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1462_length_9791_cov_3.496816, whole genome shotgun sequence.
ACCESSION   DAWBSW010000061 DAWBSW010000000
VERSION     DAWBSW010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1462_length_9791_cov_3.496816"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3062 a         2157 c         2201 g         2371 t
ORIGIN      
        1 gaattgtttt ggcgttgcga tacaatgagg caacggcagt tgccgacaac atattcaaag
       61 tcacaggttc gagctgctcg tacctcgcag tcaccctttt gtcactccgt gacatttccc
      121 ctgtcagggg aatctctgtt tgaggcgcca taaaagaaga gtaattttga tacaacatca
      181 aggttactct tctttgtttt tatttcaccc cctttgctgc ggctattttt taactttttt
      241 gtgttaatag cgaccgatat tcaaacttga aacacgtttc cgatctttct gccttccttt
      301 ttgtcaccga tattaaaaag acccaaagtg caaatcaatt catagtgata cttatgagat
      361 tgatgccttt aaaatataat tcactattaa aataatttta tataattatg catttttaaa
      421 cttaatttaa taaaatttgt gcattattta tactaaattg acttatataa tagtaaaatt
      481 atagttgaat tttgtactga attgtgatat tataatagta tctatgctgg aaaaataggt
      541 tattggattg aaaagagccg caatctaatt ttagcaaaac tttttttgaa taaaaatgcg
      601 ctgtgtgcaa tggggtgtcg gcgcgtttta gaaccatggg aggtaaatat tatgaaaaga
      661 aagctttcga aactcggaag gagtacggta agcgtactgc tctgccttgt tatgcttttg
      721 actaccttct gcttctttga tatcggttcg atgatatcgg aagcgttggt cagcaagagc
      781 agtcatgcgc tgacggaggc aggcacatcc tcggatacct ttgcacagta taatatttct
      841 gcgcccgagc ttgtctatat caaggccggc ggaaaggact cggaatactt tctgaatgca
      901 acgtcgagcg gtacggtcag cgagccgaca tcggcgacag gtatgctcag attttcctgt
      961 gcaacgtcga ggagtgttac tgtttcaata actgttcttg acgcaaacct caacgcttcc
     1021 ggtgcaaaca gagtaacaaa tgttgctttc tccgacggca catatatgtc atcggataag
     1081 gccttgtcgg cgccttcgtc atctgttacg aagacgatta agagcatttc catggcgaat
     1141 gccgaggttg gcaaagaata tgttattcgt tggaatatcg tttatacaac cgcctcgggt
     1201 tcatatacga cctatgccta tacgggcatt aaattcccgc tgctctctca ggcaggtatg
     1261 acgacaagac agtattatta cggaacatac ggcggagtga ccggtaaccc tgccgagaca
     1321 tctacgtatt catttattac gggtgcagac tcggttaaag gcggcaacgt cgcttccaag
     1381 tgggtcagca caggctcgac tcttacagct ccgcttatca atttcaatgc aggcctttat
     1441 ccgagaggac ttgatatgcc gataaattcg aactattttg atccgaacgg ttcggacgta
     1501 ttgctctgct attcaaagcg taatgacagc aattatttcg gacagggctc gagaatttat
     1561 aacactcagt gggacggttc aaatagctat aatcccaagg caatgatcac ccagtcgggt
     1621 tatttcggcg gttctccttc gacggacggt gacgcatacg gatatgcggc cgccgacatc
     1681 actatcgata catcaaggtt ttcaaatttc aatcaggttc cgaaccttta tgcaggtttt
     1741 gtagagtttg actccgacgg cggcgcaagc cagaactatt tgaacgcaat cagaaatgtt
     1801 tcttatacat cttcagatgc tgcgaaatat ttcagaaaga acaaaactat caataataat
     1861 gccggcagta tgaacacggc gccgacaatc tattgtccgg atgttgaccg ttcagatgaa
     1921 aagaacggcc gttcatgggt tcgcggcctt tacaagcttg acggcgatat aaccgcggca
     1981 aagaataatc ttacaaattc aacatcgggc ggaacatcgc tcactattgc ctctttcagc
     2041 agtaatgttt attcaactta tatccgtttt gaatattcgc ttcgtttcaa tccgcttata
     2101 ggttatgacg agtattgttc cggaatctcg gctgtaagac tcaatgccac gcttgccgat
     2161 aaggcaacgg ctcgtaaata ctacagtaaa ttcctcaatt ccggtgtcaa tgcatcggca
     2221 aacaactggt cggcaatcga ggaatatgtt aagtcatacg tcaagactct ttgcgtctcc
     2281 tctgcggcgg cgggttcata taccgaccct gcgtctgcaa tctcggcggc acagacaaac
     2341 ttaaaaacag tcatgaagac aaccactccg ttgtctccgc cgttgtactt ctatacaccg
     2401 gaagcaattt atcttactcc aagtgcatcc tcatggggag cacagacaag atcttccttc
     2461 cagtggtata ttcagaataa tatctctgac ccggcaaatc cgacaacaac gacttcgaga
     2521 gatacctccg gtacggtata tttcaattat ccgaatgctt cgaatttgag gatcagctat
     2581 aaatggatca acaagagcgg aagcgaaatg ggaagcacat cttccacatc ttccagctat
     2641 tataatacca attcctacat gacgcttggc ggctcgaatc tgtattcaaa aacagacaaa
     2701 acagtttcgc tttcttcggg catcgcgaat ctttcggtca cggcaggata cagtccttat
     2761 cttgtaagcg gcgacacagg ctgctttata gaatggacgg cgaaatatat tgatttaacg
     2821 gacggttacg agaaaacagc tatagcttat acatatgtat ataagccgtg ggttgcaccg
     2881 attgcaggtg cggattatat tggaaactat gcaggtacaa acttctattc cggtcagata
     2941 acctggatca gcggtgttca cggattgaat gctcagggcg gatattaccc cagatattca
     3001 ttctccaaca aggcaaaagg tctttctgct ttcctgagca acagtagctc ggcttacggc
     3061 gcatcaagca caacacttgt tacgggcaca aaaactcagc ccgatgtatc aaagctcacc
     3121 tcgaaaagct catatgtttc ggattcaaca aacatgtatg cctcgtttgc aacaacgagt
     3181 aatactactg ccttgttcag tgcaaatcag gcaaacagcg gtgttaataa caacacgcct
     3241 attgactgga tgcgaagcag cggttctttc acgacaaatg cttccacaac cgataacctt
     3301 tttaacgtga agagtgcagg cagcgcagaa tatacaaacg atgactccgg ttcgtccaag
     3361 tgtgtaaagg tatctgtatt taataacgaa gcaaagggat ctatttatat tgatacaagc
     3421 cgttacacaa accttaagga tattccgaac ctcggcgtcg gacttatggt tactcatgat
     3481 aaatcttccg atgccggtgc atggtatgtt tccgatatgt cgcataacac aacctacgat
     3541 ttgggccata ccgcagacgg cgtacgcaat tcaagaagcc gttcaaacta tgacaccttg
     3601 agagctgtat ttaacaacca gggcacggtt attgccggac agggaacggc aacctccttt
     3661 ggcggttatg aatcagaagg cattaaatac gcaggtattt gggtatcggc tcttaataat
     3721 acaggcagcg ccacaagtac ggaaacatac agaattaaag gcgcatatgc agctaaccag
     3781 gacagtgacc agaacctcgc tgttgttata acaaagcttg ttgcaactca gaccaacaag
     3841 acagaccttc gtaaggcagt taagcgtgcg cagaacaact tccctaatct cggcatacac
     3901 aacgctgcca ctatgtcgag ctactattac tcaggcacga catatacgaa ttttgtaaat
     3961 acatataaaa atgcttatcg tcagcttaca aaacttgacg gtaactatac cgatacaact
     4021 gcgtcagcac ttgcaaccaa cctcaataat tacattgatc agcttacaac aggaacagat
     4081 cttaaaacga actgcacagc ccgtgaatac aatctcggac ttgaaaagct tgataacggt
     4141 cagtataaga ttgttgagat cagcggcgga cagcagtatg aaataacata cgacgcccgt
     4201 gataatgtat atttcacccc cgatacatat gagggatata catatatcgg taagtgtgaa
     4261 ttgacgaatg agacaattgc tccgtcggat tattacggca aaacgcttgc ttctcttccg
     4321 acatttttct cggcaagcaa tttgtacagc aattcgatga gctattccgg ctcaaccgta
     4381 actatcggaa caacaacggt taagacaacg actgttccgc agtttgacgg agataccact
     4441 gcctatgagt gggtcgccgc agtcaatgat acttcgctga cgatggttaa tttctacttc
     4501 attgatcaaa acaagatata ttttgacccg aatgacggat caagcggagt taaccttctc
     4561 actcagccta caacatataa atcgggaaat aatttctatt ctccgacatt tacttcaagc
     4621 cccggcaacg gagtaacggc aacgttcgac caaacagaca gcacgtttac gttcaacgga
     4681 acaccgacga cgggtgcgga atatttcaga ttcccgattg caggcaagct caaggcaaat
     4741 accaattata catacagagt tgtttatgtc agcggctcaa agacacttga cggcggcacg
     4801 cttacattga cacttgatat ctgcgacaag aacggtaaca gatgttacaa ggcaagcggt
     4861 gaaaactaca gcgacagatt tgtttttgac caaaccgcaa accaggaaac aacgtcaagg
     4921 acgcttgctc ccgatgcgta ccaggcggca aatgcggaat ttgttcaggt gtttacctac
     4981 agcccgagct acggcacggt cagatacaat aactataagg ttaagattga gcttgttgaa
     5041 agcggttcgg caaatcaaac ctattctccg aatgcaattc aacagttcta tggtcacagc
     5101 atcggcacaa tgcctgtgcc tgttcgtgac aactaccgtt tcctcggctg gttcacagct
     5161 ccggacggcg gcgaccgtgt aacggcttca tccttaatgg gcagcacaga cagaactgtt
     5221 tatgcgcact gggcagtgac ggataaggtg cttcttcttg ataacgattt caactttgac
     5281 gattttgtaa ggaaggcaag caacaacaat gcatttgata cttttatgaa ttcgtacaag
     5341 gcatgtaagc ttccgggaat gaagaatcct ctcagctatc aggagaatga ttatttctca
     5401 tattcaattg caaaggacac tgagggagat accgctctga atgttaagta cgaccgaaag
     5461 catattgtcg atgccggtgt agatgaggtc tttgatacct ttaagtcgaa cacctacacc
     5521 gcttcggtgg aaggcgaata tacactcaca tatgaataca gaatcaatga ttactattca
     5581 tatataaagg atactaccgg aaagagcgag tatgactcga ttgttctcgg attcagcact
     5641 aatgacggct attggtacaa cggcggtcag ggcgtttcct ataccgaaaa cagaaaaact
     5701 accgacggtt atgaatctgt tgagttgaag cgcacgttca cagcgggaac tcaatatttc
     5761 tttaacatga tattctattt cgcatattat aataattata atgaacttcg tgcggatttg
     5821 gatatcaaga atattgttct taaagacccg aacgggaata tcgttctttc ttcaaacggc
     5881 atgaatacgg ctaacggcac atcatcattt gactccgatg caagaacgat tactctcaag
     5941 ccgacgggga cagattgcta cacaaatact tatgataatt tgtcaagcta ccgttcaacg
     6001 cttgaagcag gcaaaaagta cactcttttg atggattatc aggcaacaac ggccacaaaa
     6061 atttcaccgt ttatcttctt ctacccgaac gcaacttcaa cgagttatac ttcatccgca
     6121 gcggcttata tccaggtccc ggcaggctca ggtacggttg caatgcaatt cactgttccg
     6181 tcagataatt attatcagat aagattcagc gaaaacagcg ttgacgactc aaccgttccg
     6241 ggtgcattga ccttgggcaa tatttcgctt caaagaacag agatattctc agacggagcg
     6301 tatgtagccg attcaagcac atacctcggc tgggtcagca aggtaacagg ttcggcaagc
     6361 gcaacggcgg tttatgtcaa tgaccttaat tcaagaaatg agaaaatcgt ttctcattat
     6421 caggatattg actacggtgc acagtacgac gagctttatg taccgacccg tgagggtttc
     6481 accttcctcg gctggtatga cgcagatggt acaccgtata ccgacgcaaa cggcaacgct
     6541 gtcggcaatc atgtttgtac aacggacgga tttaagctct attcacgttg gacgcctaag
     6601 acattcaatc tcaaatacaa tgctttcaga ccgaacgaca gtgcaggagc ggtaaccgca
     6661 ggcacggtcg gcaatctccc gacaccgaat cctcaggtac ttaagttcgg tgtggaatct
     6721 tcaatcagcc tgacagcacc tactcttacg ggttatacgt tcagcggttg gtcagagacc
     6781 tacggcggag caaaggtgta tgataagggt cagactcttt ccgcttcgac cgtaagccgg
     6841 atgtataaag actgcaatgg cggaacgtac aatatttata caacttggac ggcaaacaag
     6901 tataaaattc attttgataa gaatgcaact gatgcaaacg gttcaatggc tgatatggac
     6961 atggtatatg acgttgcaaa gaatcttagt gaaaacaaat tcagcagaga aggatatact
     7021 ttccaaggct ggaacacggc gaaaaacgga agcggtacat catacgacga taaggctttg
     7081 gtcaataatc tgacagcagc cccgaacgga acagtaacgc tttacgccca gtggacaatc
     7141 aatccataca atataaaagt atattcgaag tcgaacaagt acaacaatgt aacggcgtac
     7201 acgaaggata cggtgggcgg aacggtacag attaacacaa cgcctgcgag cacaacaacg
     7261 acgctgaact acggtacaga gtacagcata accgcaacgg aaaagacggg atataagttt
     7321 gagggctggt atacgccgaa agacggtaac gatttcggaa cactggtaag cacatctgaa
     7381 acatatacga cgacactcgg agcggcaaat atagagtact atgcgaagtt caacgtaaga
     7441 acatacacga taacaataga tcctaacagt ccgacaacag gatcgaatat ttccggctgg
     7501 tatgattacg gtgatcataa gagcacgccg gcacctgtac agacggtaac caaaacaacg
     7561 tcagtcacaa tgacgtacgg ttcaacggta acgatgacag caccgaaaaa gacaggatat
     7621 accttcggcg gatggaagct cgacggaaac ggaacattga attcaacggc agatataaca
     7681 aagaattcaa catatacgtg cggagccggc agcggaactg ttacggcgat ctggaacatc
     7741 aatacgttca cgatcaagta taacggtaac ggatcaaatg caggaaccag tgtaccggat
     7801 cagacattta cgtacaatgt tgcacagaag ctcaataaaa atacctttga gaagaagtac
     7861 acaataacgt tcaacggcaa cggaggaacg attgacggaa agacggaagt aacggatacc
     7921 gcggtatgga atcccaacgg ctggaacaat aaggctgacg gaacaggcgg atattcttac
     7981 gatgatatga aatccgtaac aaatccgtgc ggacttatcg agaatgggtc aagcgcaaca
     8041 ctgtatgcac agtggaagga cggcaacctg aatctgaaaa cagcagagag aacaggctat
     8101 gttttccttg gctgggcgct tacggataat gctgaggtac cgacctacac cgtaaatcag
     8161 agcgtgaact ttacaaagaa tacaacgttc tttgcggtct gggtagaaac cgcaaccgca
     8221 caggaaagag tcaagagtat taacgtagta tcgggaataa cgataaagag caatgacctc
     8281 agtgttgaac cgatagtaaa gactcagaag aagtacttat tcctaaataa tgaggagtac
     8341 acaaatattt gcatagcata cgaaaaagca cttgacaact tcaataagtc aaagacgctc
     8401 gacaacgcca agaagctcgt agaagctgta aaggcagtcg gaagctatca ggcgggagac
     8461 tttaagtatg acgcaccgat aaagactttc attcgagagt tcgagattga atatgcatcg
     8521 ggagtaacgg gagcagtcgg tgcaggaaca cataagctca gtgaccttaa tcttaaccac
     8581 tacacaaagg aaatactcag tgaaaatgag gattcaattc taaagcaggt taagctcgca
     8641 gaagcagtaa caagcataag cagccagagt ataatcaatg aagcggtaaa gaaaatcgca
     8701 aagaattatg caaataaggt taagattagc acaacaccga cctacaaggt atatgagaac
     8761 agtgaggaaa taaagaacat actgtcctta tcagagggaa tcagcggtgt aaactatgtg
     8821 cgcacggatg atggcaatgc ggtatattac tgctatgcaa attctacgaa tccgaaaatc
     8881 taccttgagg tagaggacga aatgacaaca cagaatcgtc tttgctatcc gacaagatcg
     8941 gaagtaacgt caagagcagt tgatccggta ctcaaaccga caacgtcgga tgtaaaggca
     9001 aactttacgc ccggcacaaa ggaagtaaat aacagcacat atacaacgta tatcaacgag
     9061 ggactcggaa atacgtttac gggaacggta aacggaaaga aatttacagg actgagctat
     9121 tacaagcaga agtcaacggt ggaactttca cccgtattta ccgagaacaa gaacggagcc
     9181 gcagagtatg tgcttacatc aacggatgac agctacaaca gtgcaagcgc aacgctcggc
     9241 ggaaactacg caacaacagc aagtctttcg gcaggcatga acagcggcaa ggacttcaca
     9301 ggctcgacga aaaagacagt aacgataatt atcgactatc atgtagccga ggaaatcaag
     9361 gacaagaacg gaaatccgac aggagaattc caaaagcttg atgtaacggg agatcaggtg
     9421 cagatggata agtggctcaa ccagtaccat ctgttcagac agtccggcgg agcaaggaac
     9481 tgggagcttc ccaagaccgg agacaaggcc tacacggtaa atgacaaatc ctatggtcag
     9541 accgacagag gctcattcac attcacctat gagctcggca agacagagct tggcggagaa
     9601 aagatcaata caacggatgc aaatgagctc gtgaaactgt tcaataatga ggcgaaatac
     9661 aatgaggcaa agaagcttac gtttgtcgga acaaaccgaa taacggataa gagcagtgcc
     9721 gaagaaaaag ctaacgcggg actcggatat caggtatggg gcaacaactg gagcttcaac
     9781 tattatccga a
//
LOCUS       DAWBSW010000062         9752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1472_length_9752_cov_2.808601, whole genome shotgun sequence.
ACCESSION   DAWBSW010000062 DAWBSW010000000
VERSION     DAWBSW010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_9752_cov_2.808601"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3690..3699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4717..4726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2542 a         2140 c         2294 g         2756 t
ORIGIN      
        1 cataaaacct ctccttttcc attatatagc tattttatca gtgatggaca tgcttgtcaa
       61 tgaacaaaaa gggggaatta tatcttcttc attatctttt atgaaatggc tataaagagc
      121 aaatagaatt tttgcagcaa tacaatcttc tcgtttagaa accagaaagc ctaaaaaatc
      181 ccaaaaaacg ctcaaaaagc gtaattgtta ataaactgtt catataattg caatattttc
      241 tcagtagaga tataatatta tactcgtgga gatagttatc tctattttca tttttatttt
      301 ctctttccct tgaacggttc gatgttgcac acgtcggacc gttccccctt ttacgaataa
      361 atatgacaga aaaagacatt gtccggcctt cggacgatgt ctttactata ttatttggct
      421 tgatccttgt gtattgacac cgggtgcaaa atgatttata attacaatgt ataataagac
      481 tttttctgag gttttattaa aaaataatat aggagaagtt gaaatgaaaa agattacatc
      541 attattactg agcttatgta ttctgctgac ggtatgcagc gccggtttcg tgacgataac
      601 tgcttctgcg gcggaaaaaa tcgaaggctc tgaggtcaca tggagctttg actatcagaa
      661 caaaactctt acctttgacg gcaaaggcga cataccgaac tatgatacat atgaggatga
      721 gaacggtctt tcgcttattc cgtgggccgg ctgtgactat aatacggttg agtttggcaa
      781 ggaaatcacg ggtataggca attatgctct cagaaagagc cttagtcttg ttacggtaac
      841 gattccgagc acgataacaa aaatcggcaa ggaggctttt tcaaactgcc ttgcgctgaa
      901 gagcgtaaca ataagacccg gaattactga gataagcgat ggcgcttttg caggctgcac
      961 aggtcttaca accgttgagc ttcccgacgg aataacaact atcggattga attctttcta
     1021 taagtgcacg tcgctaaagc ttatcaacat ccccgattct gtaaagacaa ttaacacagg
     1081 ggcttttaac acatgcacgg cgcttgaagt gtttacggca ccaaagtctc ttgaaacaat
     1141 cggtgacaga gctttctatt gctgcgaaca gctcaaaacg gtcactctta acgaaagcat
     1201 gagttatatc ggtgtttctg catttgatag ctgcgcaagc ctcaagaaga ttacactgcc
     1261 ttccggtatt cagaagatat caaatgctgt cttttcgggc tgttcgggac ttgaagaggt
     1321 tcttatcccg gagggaataa gcacgattga ggataacgca tttcagtttt gttcctctct
     1381 caaaaacgtg agaatcccat atactgttaa aacgatcggc gaaaaagctc tcggctacgg
     1441 caacagaggc aagctgataa gcggatttac tgtcacaggc tatgacggaa ccgcggcgca
     1501 gaaatatgcg gcggataata aaataagctt taattctctc ggcgatcctc ttgcaaagag
     1561 cggaaacctc agcgataagc ttacttggaa aattgatgac gagggcaacc ttgttttcgt
     1621 cggcagtggt gaaataccgg attattcgct ctacgatatg ccggtttatc tcaacggaga
     1681 tctccggtcg gtaagtcttg acaaggccat aacaaagctc ggcgcatatt cgctgattat
     1741 ggattgtgag cttttttatg tcggagaaaa ggttgagtct ataggtgaaa aagcaatagg
     1801 ttatcatttt gacgaaacag gtaagcttgt taagaatgat gattttgtaa ttctcggcta
     1861 caagaaaaca gcggcggaaa tatatgccga agcaaacgga tttacgttta tgccgattgt
     1921 tgacgaggga cgctgcggcg aaaagtcgac atggagctat gacattgcct ccgagacact
     1981 tacaataagc ggcgagggcg cagttgatat ctattatgat gacaatgtaa tgccctgctt
     2041 cttaatggac ggatatacgg ttggcaaggt tataatcaac gagggaataa ccgagcttgc
     2101 cgaggacgct ttcgtcaatg tcgaaaacgg tgataaaata attacgttcc gcgttgccaa
     2161 gagtgtaaaa actatcggaa gttatgcgat cggttatgtt gcaaactatg ttttgaacgg
     2221 cgaagatatt gaggttgagt acattcagaa tgagaactgc gtagttgagg gctatgacgg
     2281 aacggcagca aaggattatg cgagcgcaaa caagtttgat tttgttgtcc ttgaccctga
     2341 aacagatcct cccgttgaag cagatacaac ctttaagctt agcgataagg cggttatttg
     2401 cactcttgac gatacaagca agattattaa gatttacgac gagaatgcaa cggcagaaaa
     2461 aataatggct gattttgttg tcggcaagga tcttgttgta agcgaaatca aagcggttgc
     2521 aacgggtgaa tctctcaaga cctcatacag cgcatcagtc tcgaacattt atacattcgc
     2581 tctcatgggc gacgttaacg gtgacggaaa ggtgaattcc gcagatgcac tctgtgtgct
     2641 caggcatgcc gtttcccttg acgttattaa gggagttaat ttccttgctg cagatcttga
     2701 cggaaacaaa agtgtaaact ccgcagatgc tttggtgata cttcgacttg ccgtaggaat
     2761 tgataagctt ggcaattttt atcccaaggc ggtaaccccg acggagccga caactcccga
     2821 aactcccgtt gagccggagt caaaataaat actataaata atttgggacg caggctttat
     2881 gcttgcgtcc atattttttg ctccgttttt aataataatc aacatatttt ttaaaaacct
     2941 attgaaattt atgttaatat gtgatatagt tagagcgttg atacattgat aggaactctg
     3001 ttaattgttg caattccgcg ttcaaaatta tttaaggtac gctgaaatca atgtagaacg
     3061 taaggaggtt attttttgaa atactataat gctaaggata tccgaaatat tgcagttgca
     3121 ggccatgcag gacgaggcaa aactaccctc gttgaggcta tgctttatat cgcaggtctt
     3181 acggacagac tcggcagagt tgatgacgga acagcggttc tcgattttga ccccgaggag
     3241 agaagaagaa atgtttcagt ctcggcagct atcgctccga tcgagtggaa agactgcaag
     3301 cttaatataa ttgatacccc gggtcttttt gactttgagg gagaaatgtg cgaaggtatt
     3361 gccgcttccg agtgcgtact tattgttctc ggagcaggtc ataagtatga cgtcggtgca
     3421 gaaaaggctt tcaaagcagc caagaagcgc ggaaagatgt tcgcaattac ccgatgcgac
     3481 aaggagcatg ccgatttcta taagacattt gaggagatca aggcagttca cggcggcgca
     3541 gtttgccccg ttatcgttcc ttacatagtt gacggtcagg taaaggctta tgttaacctt
     3601 gccgctcgaa agggctatga atatcacgac ggcgatgccg ttgagattcc gatgcccgac
     3661 gacccgaaga ttcaggagat gcttgatatn nnnnnnnnnt cttaccgagg cagttgcaac
     3721 ggcaagcgat gagctcatgg agaagttctt tgcaggtgag gaattcacac gcaaggagat
     3781 tatcaaggct ctcaatgacg gagttaatac aagttctgta tatcccgttt acgcttgctc
     3841 cggttatacg accgacggtg tagaccttct tatggacgag ttggcatatt cagcacccga
     3901 tgccgcatct tatgcaggcg gcaaggacgt tgaatgtgac gaaaacggac ctcttgcggc
     3961 tctttgcttc aagaccgttg ccgacccgtt tgtcggcaag atgtcatatt tcaaggttga
     4021 cagcggaaag atcaccccgg aaactcccgt ttacaatgcg cgcacaggcg agacggagcg
     4081 aatgggcaag attattacaa taagaggtaa taaatccgag gactgcaaat gtattcctgc
     4141 cggcgatatc ggcgttgtaa caaagctcaa ctgcgttaag acgggcgaca ctatctgctc
     4201 ggcgtcgaat gttatagaac tcagcggaat caatttcccg aagccgtgtc tttcaatggc
     4261 tattaaggct tgcaagaagg gtgacgagga aaaagttgtt tccggcatca acagacttct
     4321 tgaagaggat ccgacaatta agtttgagat gaatcatgag accaaggagc agattctctc
     4381 gggcctcggt gagcagcatc ttgacatcat tgtttcaaag ctcaagagca agttcggtat
     4441 cgacgttgag cttactccgc cgcgagttgc ttacagagaa gctatccgca agacggttaa
     4501 ggttcaggga cgtcacaaga agcagtcggg cggtcacggt cagtttggtg atgtttggat
     4561 tcgctttgag ccgacagagg gtgacgatct tgtatttgaa tctgaggttg tcggcggttc
     4621 tgttccgaag aactatttcc cggcagttga aaagggactg cgtgagtgca tgaagaaggg
     4681 cgttatggca ggctatccga tggttggtct caaggcnnnn nnnnnnctca aggctgttct
     4741 ttatgacggt tcttatcatc cggttgactc atctgaaatg gcatttaaga cggccgcttc
     4801 ccttgctttc aaggcaggta ttccgcaggc aagtccggca atacttgaac cgataggaac
     4861 tctcaaggct cttatgcccg aggcatatct cggcgatatc atgggcgacg ttacaaagcg
     4921 ccgcggccgt gttctcggta tgggcccgtc cgaggaggat cacagccttc aggagcttgt
     4981 tgctgaggtt ccgatggcag aaatggctaa ctttgcaaca gttcttcgct ctgttacggc
     5041 aggcagagga tcgttcacct ttgagttcac acgttatgag gacgctcctg ttcccgttgc
     5101 ggaaaaggtt attgctgact cgaagcttct tgaggacgat tgataaatca gaatatgaaa
     5161 caagctcccc gaccgtaaaa gtcggggagc aatttgcttg tattgtttag cctatgaccg
     5221 attccgcata gcgaacggtt tccattgcgt ctgcggaata tttgtctgcg ccgattttat
     5281 cggcatagtc ctgagtaagc acagcgccgc ctacgacggt tttgcaccac ggtgcatttt
     5341 cttttatgag cttgaccgtt tcctccattg cgggaactgt tgttgtcatc agtgcgctca
     5401 gtccgacgag cggagcatga ttgtccgtga cggctttcaa aacggtctcg ggcggaacgt
     5461 ttttgccgag gtcgataaca ttatagccgt agttttcaag caaaagcttt acaatattct
     5521 ttcctatatc gtgaatatca ccgtgaacgg ttgcaattac aatgcttcct tttttgacac
     5581 tgctgccgtc cgccgacata gttgccttga taacctcgaa tgaagccttt gccgcttctg
     5641 cactcatcag aagctggggg aggaacagct ttttttcttc aaatcgcttg cctacgttgt
     5701 caagggcagg tataacatga gcgtttacaa tgtcaagcgg cgcactattg ccgagcatgg
     5761 ctgttgttat ctcgcttgcc ttttccttga ggcccttttc aatcgcctcc ttgagtgtaa
     5821 gtgagctttc ctgtttagct gtcggctgaa cctcctgccg agaagcaaaa ttgataaaat
     5881 ccatgcagtt ttcgtcaagg cctttaacaa cgttgaagct gtaatatgcc tccatcattc
     5941 tttccgagta cggattcata attgcggcgg aaagtccgtt ttccatcgcc gtggtgaaga
     6001 atgccgcatt aacaagatct ctcgacggta agccgaacga aacattggaa acaccgagag
     6061 aggtgttgca gccgagctgt tcggtgattt ttttgacggt ttcgagcgtt gtaaccgccg
     6121 acatcttatc tgcgcttacg gtcattgcca gcgggtcaaa tataatatcc tttttgttga
     6181 ttccgtataa tgcggcggtc aatagaattt ttcttgcaat tttcattctg ccatcaaccg
     6241 ttgacggaat tcctttttcg tcaagcgtaa gagcaacgac aaaaccgccg tactttttca
     6301 cgagaggaaa aattgcattc aggttttcct ctttgccgtt gaccgagttt atcattgcct
     6361 tgccgttgta gcggcggagg gcactttcca tggcaaccgg gtcagaggaa tctatctgca
     6421 gcggcagatc cgttacgcat tgaagctcgc aaacggaatt tgtcagcatt tgagcctcat
     6481 caattcccgg cagaccaacg ttaacgtcaa gcacatgaac tccctttgcc tgctggttta
     6541 cggcttcctg aagaatgtag cctatatcgt tttctaaaag agcctgttta aagcgttttt
     6601 ttcctgtagg gttaattctt tcgcctatca agatcggctt ctctccgaag aaaacggctt
     6661 tgttatatga tgtgcagaca gaatatgtct ttttctcgat ttctttcggt ctcatgtcac
     6721 gggtaagctc gcaaactttt ttgatatatt cgggcgttgt accgcagcag ccgccgacaa
     6781 ttcttactcc gtttgaaacg gcaagcttaa tgtcctgtgc aaattcttct gcatcaacgt
     6841 cgtagtatgt caccttgccg tcgcttttcg gcagaccggc attcggcttg aaagcaacgg
     6901 gaacggagca gtattttaaa agctcatcca taacacccat gagctgcttc gggccgagcg
     6961 agcagtttgc gccgattgcg tcaacgccca ttccttcaag catagttgcc ataaccgcag
     7021 gatcggcacc cgtcataaga cgtccgtttt caccgtatgc gtttgttacg ataatgggga
     7081 ggtcactgtt ttcttttgcg gcaagcacgg cggcctttgt ctcatagctg tcgttcattg
     7141 tttcgattgt gataaggtca acgccgtatt ttacaccgag acttattact tcggcaaaag
     7201 ctttaaccgc atcctcaaag tcgagatcgc cgaggggctt aagcagctta cctgtaggac
     7261 ctacatcaag cgcaataaat ttttcctgct ttcccgaaga ggcttttctc gcttcgtcag
     7321 cgttttttac ggcatgatat ataatttcgc taagctcatc tgtgccgaat ttgagagagt
     7381 ttgcgccgaa ggtgttcgta tttacgatat tactgccgct gtcatagtag cttttgtgga
     7441 tttctttaat cacctcgggg tgagaaatgt tccaacgctc gggcagctcg cccggctgca
     7501 aaccgctttt ttgcagaaga gttcccatgc cgccgtcgag gtaaactata ttatttttaa
     7561 taaaatcacg aatattcatt gaaacacttc ctatcattgg gttattccga taaatgctgt
     7621 tactgacttt gacggtgaca ttaaaagcgt atcaccgagt gtgacgccga ttcttttggg
     7681 gcagtcaagc agcgagaaaa tcattttttg cgtttcaagc ggcaggtctc cgtagccggg
     7741 gctgaatctc ggtcggagct tttctccttt tccttgaaat tccgattcca ttttctcgca
     7801 aaaatcattg cagagagctt caactctttc cgctccgatt gcctgaaggc ataaagcctt
     7861 tgcgggcgat aatcttgaat attttgagat caatcggtca atgccgatgc cgattgttgc
     7921 ggcaaagatt attgctttat cgcagccgct gatatttttt gcaaggtttc ggcttgtgat
     7981 ttgtccgcat gacagagaga tgatattgct gtcggtttca gccgtcagaa tggcgtagca
     8041 aaccttatat tgaagcattt cccgaacctc ggcgagacat tcgtcaacaa gtccgagaat
     8101 ttcatcaccg ccgccctgcg acgacatgta tcgcattatt tcttttttat ttatgctcgg
     8161 ttctttgaat gataagctgt aaaccgtttc cataatatca cgtgcctaaa atatcagaga
     8221 gattggactg tattttctgt gcaacatcgg gcttgttcat tgaataaaca tgaacgtttt
     8281 caaccccgtt tgcgtaaagg tcgatgatct gatccgtcgc ataggcaata cctgcctgct
     8341 tcattgcaag aggatccgaa ccgaacttat caacgaggct tttgaatctc tgaggcatga
     8401 atgagccgga aagtttgatt gctctgtcaa cctgttttgc atttgttatc ggcatgattc
     8461 cggcaacgat tggcacggtg atgcctgctt cacgaatttt gtaaagaaaa ttatataaaa
     8521 gattgttgtc aaaaaacatc tgcgtcgtca ggaattcaca gcccgcgtca accttttctt
     8581 tgaggtgctt aatgtcctcc ttttgattgg agctttccgg gtgaatctcc ggatagcatg
     8641 cgccgccgat gcagaattca aagccgcttt ccttaatttc acgaataagg tcaacggcat
     8701 atcggtgatc ccagccgctc ctgtcatcag cctccatttc tgtaggaata tcacctctga
     8761 gagccagaac gttttttatc ccacgcttgt tcatctcgaa aatcctatct tgaacggttg
     8821 cctttgacga ggaaacacaa gtcagatgcg caagcgtcgg cacgccgtag tccttcataa
     8881 tatcctcggc aatgtcaagt gtatatttgc tcgttcctcc gccggcaccg taggttatac
     8941 tcatgtagga aggcttgagc ttcgcaatct cacgggttgc ctgctgaaca ctttcaaagg
     9001 cagtgtccgt tttgggcgga aaaacctcaa aagataaaga catttttccg ttgttgagaa
     9061 tttctgtgat tttcatatct gtcgctccct tcggggtcaa gttacttctt ttcttttaca
     9121 ctgtcaagac catagtgcat ggcaaaagtt atgttgcgcc aatgctcggg gtcctcggtt
     9181 atgtctgcgc cgtccattga tggggcatgc tgatccgtag tgtaatattt ttcaagctca
     9241 aggtcatagt tgccctgcgc cttctgctct tcaatatatg catcacgctt aacgttaaag
     9301 gaatgaaggc ttttcatgtc ctgcgccgtg tgaacacatg acatcatgct ccatatcgac
     9361 cagaacacga ccgaaatcaa aattaacctg cgtgcaagat tttcgttgag gaggattcgg
     9421 taaacaagaa ttccgaccgc tgtcattgcg tagatataaa ttccgaacca tgcccttgtc
     9481 ggaaactggg gagaggcgac cataactgct gccgcaccga atgcaccgat taaggcaatg
     9541 gctgcaattc ccgttttttt gttgccgctt tcacggtcat atgtgtaaag aataattaca
     9601 cacacaacga acgctccgac gaaaacagag aggaataaaa ccatgttcgc gggaatgtta
     9661 accagccttg ttgcaatcga attgccgacc tcgtcaccga tattgacacg tcttgaattt
     9721 cccggtgccg aaaccatgaa gaaaaatccg ca
//
LOCUS       DAWBSW010000063         9665 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1487_length_9665_cov_2.397711, whole genome shotgun sequence.
ACCESSION   DAWBSW010000063 DAWBSW010000000
VERSION     DAWBSW010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_9665_cov_2.397711"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2299..2308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3090..3099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4635..4698
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6554..6587
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2626 a         2244 c         2173 g         2504 t
ORIGIN      
        1 ctttccgatc cccgtgcggt tattctcaag gaagaggctc gcaaatacgc cgaaaagacc
       61 ggcaacacgg aaaaattcaa tcttattgat gccgttgagc gcctgactcc cgaagtattc
      121 ctcaaagaga agggcaataa aaagcctatt tgcgccaacg ttgacctcta ttcgggcttt
      181 atttacgaga tgcttcgtat ccccgaggat ctttacactc cgcttttcac gaccgcaaga
      241 atcgcaggct ggacggctca ccgtcttgag gagcttgcaa caggcggcag aattatccgt
      301 ccggcctata agagcgtaat ggcaagaaga aaatacgtca ctatcaacaa ccgaattgct
      361 aaatatgctc ccgaccaggg atatgtacct tatgaagaaa gaattattca gggggaataa
      421 aaaatgaaaa gtaaaagtga tgtaatttac aagcttccgc ttttggtttc tctttgcgca
      481 ctgcttgttg cggtgccgct gagggtttat cagtatttca aggtccttga acccgaaacg
      541 ggcttctact ccacgatcga tttctcggtc tatattatct atgccctgct cggaatcgca
      601 gtgattaccg caattgtcat cccgcttatc aacaagaaaa aaatgataac cgttgcatcg
      661 gtcaaaaaat cgcctgtttt tcttgttctc tcgctgattc ttgccgttac gataatcatt
      721 gactcggcag gtcagctcat gagctacttt gacctttatg acgccgccgc ttcttccggc
      781 tcaacaattg ccgaatacgt caagaagcag ggaggaacac ttcttcttct ccaggctgtt
      841 tcgggggcaa ttgccgccgt ttacttcttt gtctccggcc tttcggtcag tctcggaaat
      901 tccgacggct ccaagctcaa gcttctcggt ctgactcctg ttctctggtg cattttcaga
      961 cttctctaca agttcaagag aactataagc tttatcaatg tctcggatct tctgcttgaa
     1021 ctttttgaaa tcgtatttct catgcttttc ttctttgcat tggctcagac cgttgcgaga
     1081 attgacgtta aaaccgtctt ttggaagatt tttgcctacg gaattcctgc cgcaatgttt
     1141 gctctgatgt gtttcctgcc gagattcatt ctcatggtaa ccggcaaatc cgaacttctc
     1201 aacgatcact acggcgtttc ctacagtgac ctcggcgcgg cagcttatat aatctacaat
     1261 cttctttcga gagccaaggc tcaaacgagt gaaccggagg aataatcggg gtagtttatg
     1321 tttcttgaca attttattat tgcggcacgt caggtaggca tactgtatgt catggtgctt
     1381 gtcggagttg tctgcgacat aataggtttg ttcaccgaga aaacgggaaa agcctgcacc
     1441 gacttgcttt tctatataat aacaccaagc gttattatca actcgttctt cacacgggag
     1501 ttcaccaagg acagcggaat gaggcttttg attgctgtcg gatgcggatt tctcatgcat
     1561 tttatagcga ttgcaattaa cactcctttg ttttccaagg gtgaccgtga cgaaaactgc
     1621 gtatacaaat acggcgcaat ttacggcaat gtaggctaca tgaccctgcc gctgaccgaa
     1681 gcgctgctcg gcagtgaggg tgtgttttat tgctcggctg tcgttatggc attcaacgcc
     1741 gtttctttta cccacggcgt tttcatgatg gataagggta agggcttcga cgcaaaaaaa
     1801 ctgattctca acccgggcgt tatcgcaatg ctcataggtt tgccgttttt tctgttcaag
     1861 gttcctcttc cggagcttgt aacaaagccg gtcgatttta tagcgaacac tcagacgcct
     1921 tttgcaatga ttattttcgg cacatttctt tcgcatactc ggctcaaaag tcttttaaaa
     1981 cacaagaaga tttttcttgt agctctgtca aagctgatag ttttgccggc tgtcatgata
     2041 gcgatataca aactcatcgg tctcggcgga acgcttttga ctgcactcgc gatatcctcg
     2101 tgtgctccgt cggctaacaa cactgtcctg ttctccgcca aatacggcaa tgacacaggg
     2161 cttgcatcgc agcttgtcgc aacggtaagc ttcatgtcga taataacaat gcctttaata
     2221 attgcggcag ttgaaaccgt cgcataggat ttgataatat gataaatgat atcatttctg
     2281 caatttctcc tgtttacgnn nnnnnnnnct tctgtgattt tgaaccgttt cggaacaaac
     2341 ttattaactg ccgtgcaaaa ggcagacttc ccgagaatgc caaaagcatt atagtcatgc
     2401 ttttccccta ttatctcggc gaggacgaat ataaggatat caacatctca cgctatgccg
     2461 ttgtcaagga ctatcacctt gcaatcaatt cggcggtcga gggaggactt caaaagcttc
     2521 gcgaaaccta tcccgatgag caattcgttt ttttcaccga caattcaccg attcccgagg
     2581 tgcaggcggc tatggatgcc ggcttgggcg ttaagggaaa aaacaggctt ctcatcaata
     2641 aagactacgg ctcatggttc ttcatttcgg aaatagtcac aacattgcgg ctgcctgcgg
     2701 cggagataaa agaaacggaa tgtctcggct gcggaaaatg catctccgcc tgcccgactc
     2761 atgcactgag cgaaagcggc ttcaataaag agctttgcct ctcggacata actcagcgca
     2821 agggcgagct gacacccgag cagaaaaaaa taattaaaga aacgggatgt gcctggggct
     2881 gcgatatatg ccaaacggtg tgcccgatga ataaaaaagt aataattgat cccgctgaag
     2941 tttttcttga cggaatagaa acgacggcaa gaacagataa tatttccgac agagcctacg
     3001 catggcgcgg cgaaaaggtc ataaaaagaa accttgatat aattagcggg cagtgaaagt
     3061 ctgaaaaaaa tttctttcgg tgcagaagcn nnnnnnnnnt tggtaaattt cttctcacct
     3121 tagatatgaa ataaatactg aattggctta aaaggcaata aatcatatat ctttttacca
     3181 aacacaacag gaggtaaacc atgaaacttt taattgcagt catcaacagc gacgattcgc
     3241 atattgttct cgacgagata acgaaggcag gcttctatgc aaccaagctt tccacgacgg
     3301 gcggctttct ccgtgccgga aacctcacgc ttctcatggg agttgaggac gaaaaggtag
     3361 acgagcttct tgaaattctc agaaccaact gtcgcaagcg cgaggagctg acaccggtcg
     3421 ttccgacata ctcctcgggc cttgtaaatg caatcccggt caaaatcacc gtcggcggag
     3481 caaccgtttt cgttctcgat gttgaccaat tctataaaat gtgatgatta accttaacga
     3541 catcggaatc caaaacagtc ttgcagaaaa tcttgcagga ctaataaaga acgggcgtct
     3601 gccgcatgct ataatgcttc gaggtggcag tgagactctg cgccaaaagc tttcgctctt
     3661 tctcgccgaa gcatttgttt gtgagggcga tgcaaaaccg tgcggcaaat gttctcactg
     3721 tattaaggct cagaacggaa atcatcccga cgttataacc gctgaccctt cggcgcaggg
     3781 cgaaaagatg ttcaaaatcg cacttgtccg tgaaattaaa gatgatgcct acattatccc
     3841 gaatgaagca agaagcaagg tatatattct ccgctcggca gataaaatga acattcaggc
     3901 gcagaatgcg cttttgaagc tcattgagga gccaccggag tatgcgagat ttattctcga
     3961 atgtgattcc tctgcagcaa tgcttgaaac aatcatgtca agggttactc tctttgacct
     4021 cggcgcagac aatgatgaga tatccgacga actgagaaaa aaagcggacg atactgccgc
     4081 aaagttagcc tccgctattc tcaaaccgac agagtgtgaa ttcatgaaaa tcacggctga
     4141 atttgaaaag gacaaaaatc tttttgagcc taccctcccc gccctacagc tcattttccg
     4201 tgacgcagtt gttatcaagg ccggcagtaa tatcactctt tccgaccatt cggacatcag
     4261 ccgcatggcg gcatcaaagc tgacgctgaa ttcactgatg aaaatgaccc gtgaaattga
     4321 caatttcatg gaaagtataa acagaaacgc taataaaaat ttactgatca cccgattttg
     4381 tgcagtcatg cgcagctcgg cgtacaacag gtagttttat tatttttatt atatagatac
     4441 tttacataag gaggtttaat atgtcagagg ttgttggaat ccgatttaaa gaagtcggaa
     4501 aaatatatta tttcgacccc atcggattta agctcaataa gggtgaccga gctatcgtcg
     4561 aaaccataag aggccttgag tgcggcgaag tcgctcaggc aaacaaggac gtaaacgatg
     4621 acgagatcgt caaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnncc cattgtccgt gttgcaacgg cggaggatct caaggtcgtt
     4741 gaaagaaaca aaaagaagga aaaagaagcg ttcgacatct gtcttaaaaa gatcgctctg
     4801 cacggtcttg atatgaagct tattgacgtt gaatatacat tcgattcaag caaggtgatt
     4861 ttctacttca cctcggacgg acgtgttgac ttccgtgagc ttgttaagga tcttgcaagc
     4921 gtattcagaa taagaatcga gcttcgtcag ataggtgtca gagacgaatc gaagatgaag
     4981 ggcggtctcg gaatgtgtgg cagacccttc tgctgcaaca gctttctcgg tgacttccag
     5041 cccgtttcga taaaaatggc aaaggagcag ggtctttccc tcaacccgac caagataagc
     5101 ggaacctgcg gcagacttat gtgctgtctt aaatacgagc aggaatcgta cgagcatttg
     5161 ctcagggtaa ctcccaaggt cggcgcaatc gttgacacaa gctcaggcag aggaacagtt
     5221 gtcgaaaccg atctgctcaa aggaatgctc aaggttcgcc ttgaccgcag tcccgaagcc
     5281 gcaccggcag ttttcagccg acgtgacgtg aaactcataa aggacaaaaa aataaccgta
     5341 aacaaatccg aattagctgc tttaaaggag atcgaaggtt aagaggtcat gaaaaagatc
     5401 aatttaaaaa tcggcggaat gagctgcagc gcatgctcaa acggacttga aaaatattta
     5461 aacaaacaaa ccggggttat ttctgcaagc gttaacctcg ttatggctaa cgctctcatc
     5521 gaatatgatg agagcgttct caataggaaa aaaatagaaa aatttgtaaa ggaagcagga
     5581 ttcgaaagtc tcggtctttt tgacgaattt gagaagaaca gctcacccaa aatgtcaaaa
     5641 ttatggtttg tcataatgac agctttggcg gtaatcctta tgtatatctc catgggctca
     5701 atgataggcc tgccggaaat cccgtttctt gcaatgcaca cccatgataa agcgttcgca
     5761 tcgtcactgc ttattctgac ggtaccgttc cttgtttacg gctttgacat catcaagagc
     5821 ggattcaaaa acctgtttca tcgcacgccg aacatggaca ctcttgttac tctcggcgtt
     5881 ctgacaagcc ttatttacag cattttctcg gttgtgatga tattcatggg acatcaccac
     5941 tatatccatc ggctctattt tgagtcggcc gcaatagtta tctacttcat caagctcgga
     6001 cgttatattg actctgtgag caaggacaag acaaaggaag cgattcaaag ccttgttcaa
     6061 ataacaccaa aggatgcagt tatcaagact gccgagggcg agaaaaaagt cacgattgac
     6121 gaaattcaaa agggcgacac cgttatttgc aagcccggtg aaagaattgc cgttgacggc
     6181 acgataacct tcggcaaaac tcaccttgac gaatcattta tcacgggcga gagcaagccc
     6241 gccgcaaagg aaatcggagc aaaagtcatc gccggaagta tcaactacga cggctacatc
     6301 gaatattccg ccgaacgaat aggcaaggaa tcgacaattt ccgaaattgt tcacattgtt
     6361 acagaggctt cgtcggctaa gattccgctt gcaagaatcg ccgacaggat aagctcttat
     6421 tttgttccga ctattatcgg aatcgcaatt ctcagctttg tgggctatct tgccgcaggt
     6481 cagggcttca acgccgcact gaatacgttc gtaacggttc ttgttgtcgc ctgcccgtgc
     6541 agtctgggtc ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag gtcgatgcgc
     6601 caaaaacggc atactcatca aaaagggaga tgtccttgaa acggcgtcaa aaatcaacaa
     6661 agtcattttc gataagacag gtactctcac ctacggaaat ctcaaaattt cacgagttat
     6721 cgacttcggc aaaatgcccg aaaaggacat gatgagcctt gtctgctcgg cagaagcgaa
     6781 gtcgagccat ccgatatcga aggccttcgc cgaatatgca aaggacaata ttctcacact
     6841 cagcgaaccc gagcaatttg aaaacatcga cggcatggga atccgcaccg ttatcgacgg
     6901 caaatcatat tatttcggca acgcaaagct gcttgccaaa tacggcattg agaacgaccg
     6961 cctcgatgac gcaaacgctc tcgccgatga gggcaacacc cttgtctatg ttgccgacga
     7021 ggtgcaaatt ctttcaatca tcggcatcaa cgatattatc agagataacg ttgacaggct
     7081 catcagaacg cttcgcagct ttcacgtcga gccgataatg ctcacgggcg ataacgagca
     7141 gacagcaaag aaaattgccg cacaggcggg aatagagcac gttatttccg gtgttctgcc
     7201 tgccgaaaag ggcaacgtta tcaaggagct taaagccgag ggcgacaagg ttatgatgat
     7261 cggcgacgga atcaatgact cccctgccct tgcactgagt gatatcggcg tcagcgtcaa
     7321 cagcggctcc gacattgcaa tgaactccgc cgacgtaata ctgatgaacg acgacctgat
     7381 gaaaattccc gagcttataa gaacaagcag ggcaacggtc agaaatatca agcaaaacct
     7441 tttctgggct ttcttctaca actgcctgat gattccgatt gcaatcggcg cactaaagca
     7501 tttcaatata accatcaatc cgatgatagc aagccttgca atgacgcttt cgagcttcac
     7561 ggtaataatc aacgccctcc gattgagagg gtcgaaaaaa taaggaggac tcaatatgtt
     7621 attcggtaaa gaaaaaatta caaaggaagt ttatatcgac ggcatgagct gtatgcactg
     7681 cgccgccaag gttgaaaagg cgctcgcctg cgtttcggga gttgcaaacg ccaaggttga
     7741 ccttgagaat aaaaaagctg ttgttaagct caagaaggac gttgacaatg cgattctcaa
     7801 atccgcactt gaggacctcg gctacagcgt cacggatata aaataacaac aagacaaaga
     7861 atcaccctcc gcatctaagg ccaagggtga taatttttta taccgtgaac gggtcgatgc
     7921 cgttcgtgtt tgtcggaaga attttgaatg tgaagttgaa cgttttgtcg ttcacactgt
     7981 atttcgggtc gatatccgga ccgcacgctc ccgagcccgt tccggtcatc ttatagtcga
     8041 tgcttaatac cgtctctttc atcggttcaa gctcgtaatt gtgctttgcc tcacgaatca
     8101 tctgcggcgt gaagtgctga gcatttgatg agaagctgtc gaaatcctcg gctcggcata
     8161 taaatccgat gccgctctcg tttgtaacct ttgcccactt gcagccgtaa tggcttgaat
     8221 tctcctgcgg acggacatag tgctcgaagt tttccgttac tgtcgttgtg aagtcgtcaa
     8281 cataagtata acgcttcttg tcgatgtagc tctccttggg gccgtaaccg aaatattcat
     8341 agttctcgaa gccttcgggc atgatgagct gaagtccgat tctcggcaga atatccattt
     8401 tctccgcctt cttgccgttg acgttaacga gaacgcttga atctggaagg aacgtgtaag
     8461 taaccttacc ctcaaatatc ggctcaaatg tgtaaacacc gattgcaaga tcaacggtaa
     8521 tttcaacctt atcgtctgat tttgtcacat tgagcgaacg gcacttctga ccgagctttt
     8581 ccatgccgac cttgtaccat ttttctctgt aatactgaga atcgttgtcg atatatgctc
     8641 tgtaaaggtt aatttcaacg ggcttcgaga taagctccgt atcgtcgcag cttatcttat
     8701 tgagcttgcc gtaagcctta tcgaaaacat atttaacact gccgacacct atttctacat
     8761 atctttcgcc gtcgttgact gtcattgcgg tcgtcgcaag cgcagctttg cctgtcggaa
     8821 ttgactcaag tctgaactgt ttaagtccta tctcgtagcc tgcctttgcc cagagcttgt
     8881 ccgtcctgtt gacaaagtga agcgtgagat atgtctcacc tgtaaagctg tatgccgacg
     8941 ggtcgaagat tgaaaaatcc ttcttctctc tcggcatgag cataagtccc gagacttcac
     9001 ccgaagcaat aagcttgccg tttgatttga tttcccagtt tatgcctaca tcgtcaaggc
     9061 ccttgaagaa gttgcggttc ttgattgaaa cgttgccgtt gcctgcatat tctgcaaaga
     9121 aaggctgata aacgtacttc gcttcctcaa atcccgtgtg cggcgttcta tcgggataaa
     9181 caaggccgtc aacgcagaag ttgcccgcat tcggcatatc accgaaatcg ccgccgtagg
     9241 tgtatttata tttgccgttg ccgtcgggaa ttgctacgga atgatccgtg tactcccaga
     9301 tacagccgcc gcagaatttg tcgtcggagt caataagctc ccaatagtca ccgagatctc
     9361 ccggtccgtt gcccatcgca tggcagtatt cgcagagata gagcggcttg tttttgcgct
     9421 ttttgctcga aaggatttcc ttgcactttt cgattgaggg atacatccag ctttcaacgt
     9481 cggagcagtt gccgaggaac ttaccggcaa gctcggagta gcgatagttt gcgttctcat
     9541 agtgaacgat tacgttctta ttacgtgact tgataaagtc atacattgcc tgatggttat
     9601 cgccgcatcc ggattcattg ccaagcgacc agaaaataac cgaaacatcg ttcttatcac
     9661 gttca
//
LOCUS       DAWBSW010000064         9658 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1491_length_9658_cov_2.431740, whole genome shotgun sequence.
ACCESSION   DAWBSW010000064 DAWBSW010000000
VERSION     DAWBSW010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1491_length_9658_cov_2.431740"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1812..1821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2236..2245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2859..2955
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3349..3358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3774..3783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6036..6045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2703 a         2111 c         2478 g         2219 t
ORIGIN      
        1 aacgagatcg gtaagaacat tacggtttat gaatttgaga acgatatcgt aatagttgac
       61 tgcggtcttg cttttcccga ggcagatatg ctcggagttg accttgttat tcccgatttc
      121 acctatcttg aggagaacag agagaaaatc aagggcatcg ttataaccca cgggcatgag
      181 gatcacatcg gcgcacttgc ctatcttctt caaaaaatca acattcccgt ttacggcaca
      241 aggctgacaa tgggtctcgt tgagggcaag cttgaggagc ataagctcct tgacggcgca
      301 aagctcaatg ttatcaagcc gggcgacaca ataaagctcg gaaaatttga cattgaagcg
      361 attcatgtta accactcaat tccggattcg cttgcctttg cgataaagtg cgaggcggga
      421 gttattgttc agatgggcga tttcaaaatc gacagcacac cgatcgacgg cggaatgatt
      481 gatatcaacc gcttcgccga gcttggcggt gagggcgttc tttgtatgct ttccgattcg
      541 acaaatgccg aaagaaaggg ctatacggaa tcggagcgtg ccgtcggagc atcgctcaga
      601 acactctttg agcaggcggg caagagacgg ataatcattg cgaccttcgc ctcaaacgtt
      661 caccgtgttc agcagatcat cgaccaggcg gagtcgctcg gacgtaaggt tgctctctcg
      721 ggcagaagcc ttgaaaacgt tgttgaaatc ggttcaagac tcggttatct caacgtcaag
      781 gagggtgagc tggtcagcat tgacatgatc aacaagtacc ccgacgacaa gatggttatc
      841 atcacgacag gaagtcaggg cgagccgatg tcggcgcttt cgagaatggc tttttccggc
      901 cacaggaagg tttccatagg ccctaacgac tgtattataa tctcggcaaa tccgattccc
      961 ggcaatgaaa agacggtcgg aaacgttatc aatgagctga tgaagctcgg cgcagacgtt
     1021 atctacgaaa agagctacgg cgttcacgtt tcgggtcatg cttgccagga ggagcttaaa
     1081 ctgatgatgg gtatcatcaa gcctaagttc tttattcccg tccacggcga gcagaagcac
     1141 ttgagaaagc acgcacagct tgccatgaat atgggtattc cgaaaagtaa catcttcatt
     1201 cccgacaacg gagtttgtct tgagctttcc gagaacggaa tcaagcagct tgagaatatt
     1261 ccggcaggtc aggtatttgt tgacggatcg ggcgtaggcg acgtcggaaa cgttgtcctc
     1321 agggacagaa agcgcctcgc cgaggacggc ttaataatcg tcgtcgcaac aatggattcg
     1381 tattcgggcg agattatggc aggtccggat atcatgacaa gaggcttcgt ctatgtcaga
     1441 gaatccgaag agcttattga cgaagcaaga gagattgcaa gatatgcaat agaaaactgc
     1501 atttataata atatccgtga ttggagcacg attaaatcca aggtcagaga tgaggtctcg
     1561 cacctcatgt acgaaaagac aaagagaagc ccgatgatcc tttcgattat catggaggtt
     1621 taatttatga aggacaagat caaatcattg atacgcagct ttcccgttat ttgcggcgct
     1681 tcggcggcag cattgctgtt caccgtgctg acgtttattc tcggtccgct gtggcttgcg
     1741 atagttgagc tcattgccga tatagccgta gtggtttatc ttatcctgaa ctatgatttg
     1801 atgagtatca gnnnnnnnnn ntgattaccc atatctcttc caatctcgaa cagtatgtta
     1861 aaaatatttc cgacggcttt ccgctgccga taatcatttg ctcgtcagac ggaaaaatcg
     1921 agtggttcaa cgaccgcttt gaggaggcga tgggtcctca ttcctccgga tacggcgagt
     1981 taagctcggt gtttgaaaac gtcggaatag attctatcct tgacgcttcg gtcaacggcg
     2041 ttgttatcga ctgcgacgaa agaagcttca acgttttctc ccaccgtgca aaggtcggcg
     2101 agagtgagta tatcgtgatg tatcttgtgg atatcacgaa gtacaggcat attgccaacg
     2161 attattttcg ctgccgtccc gctcttgcaa taatgactat cgacaatatg tcggaaattc
     2221 agcaggatca cagggnnnnn nnnnntcatc agcaaaataa gcgacaacag cttctatgtt
     2281 gtagccgaga agcaggatat tgacgacatg atatcgagaa agttcgatat cctcgataag
     2341 ataagaaatt acacctacga cggcaaatat gtaggcgtaa cgctctctgt cggcgtcggt
     2401 acgggcgcgg acgttgccga gtgtgagaag aatgcaaagc tttcgcttga tatggcactc
     2461 ggcaggggcg gagaccaggt ggttatcaag aataaggata aatacgattt cttcggcggc
     2521 gtttcaaaga gcgtcgatac gggcagcaag gtcaaatcaa gagttgtcgc ctctgcgctc
     2581 gccgagctta ttcaaggctg cgaaaacgtg tacgtcatgg gtcacagatt cccggatttc
     2641 gatgcgatcg gctcggcttt cggagtggta tgtattgcta aaaagctcgg taaaaacgca
     2701 ttcctcgtta cggataagga aaagacaatg gcaaagccgc ttgtcgaaag agcggaaacg
     2761 gagttacccg gcgttgttgt ttcaaccgag actgcgaagc ttgaatttgc caagagcaaa
     2821 aaaaatcttc ttatcattgt cgatacgcac attaagagnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnttgac gaaacgggca gctttgttgc cgacgcactt ctttcgggaa
     3001 ttatgcttga tacaaagaac ttcattcttc gtgtcggcgt aggtacattt gaggcagcgg
     3061 cgaagcttaa agacttcggc gccgatccga taagagtcaa aaagcttttc tcgaacgaca
     3121 ttgaaagcta ccatgagagg aaccgaatcg ttgactcggc gagaaagtat aagaactgcg
     3181 caatagccgt cgcagatgtt gaatcaaagg acatcagact gataagcgca caggctgccg
     3241 acgagctttt gacaatcagc ggggttgacg cttcgttcgt tatattcacc ctcggcgatg
     3301 ccgtttgcgt ttccgcccgt tccttcggcg cggtcaacgt tcaggttann nnnnnnnnat
     3361 gtttatctca ggttattatg gaatacatga acggcggcgg acaccagacg atggctgccg
     3421 tgcaggttaa aaattcaacg gttgcagagg tcacggaacg tgttatgcac tgcattgacc
     3481 gctacaacag ttcattaaat aaaagcgagg tttaatttat gaaagtaatt cttactcagg
     3541 atgttaaggg tctcggcaag aagggcgagc ttgttaacgc ttccgacgga tatgcacgca
     3601 acttcctttt cccgagaaag cttgccgcgg aggccaattc acaggctatg accgaactta
     3661 agaaccgtga ggactcaaaa aagcataaga ttgaggttga aactcagcag gcgaaggctt
     3721 ctgccgacaa aatcagcggc aagaccgtta aggtaaccgc caaggcggga cagnnnnnnn
     3781 nnncggctaa ggaaatcgcc gcagttatga aaactcagtt cggcattgat atcgacaaga
     3841 gaaaaatcac tgttgacgac atcaaggcct tcggcacata tcccgcaacg gttaagcttt
     3901 atcagggcgt tacagccgag ttcagcgtta cggtgaccga ggcataaata taaatcggag
     3961 ataaaaaaat ggataataca aattattcaa gtgaagctgt tttcagcctt gaggcggagc
     4021 aggcggttct cggctcaata ctgatggatc ctgcctgtat gccgcaggtt caggtctatc
     4081 tgagcgccga cgatttctat ttgccgcagc acagggcaat ttacaccgcc atagcgacga
     4141 ttgaagcgtc gggcggaaaa attgatccgt taattgtcct tgacagtctc gttaaggata
     4201 aggtatatga tactgcggcg ggaaagaact atcttttcca gctttcacag tgcgttccgt
     4261 caaccgcaaa cgttgagtca tatgcaaaga ttgttaagga gaaattctac atacgctctt
     4321 taataaacgc ttccagagag ataatcgagg atgcctcaac tcaggagcag gcggcggacg
     4381 aactgcttga ggctgcggaa cagaagatat acaatatccg tcagggcaaa aagaccaatg
     4441 cgccgtcaag aataggcgat atcatcgtcg gtgaggtcta tactacgctc cagcagttga
     4501 cctcgcccga aaaggattta tacaagggct acccgacagg ctatgccgat cttgataagg
     4561 tgctcacggg cttgcataag tccgacctca ttcttatcgg tgcacgtcct gcgatgggta
     4621 aaacgagctt tgctttaaac cttgcaagga acgttgccgt tatcggcaag agaaaaacgc
     4681 ttatgttctc tctcgaaatg acgaaggaac agcttgcgat gagagtcctt tcaaccgagg
     4741 cgagagtaaa ttcagccaag atgaggacgg gcgagcttga cggcgacgac tgggagcggc
     4801 tcggatatgc gacgaacgct ctttcaaacg ttgagcttta ttttgacgat acaagctcca
     4861 taaccgttcc ggaaatgaaa gcgagaattc gacgacttcg tgacgttgaa tgtgttataa
     4921 tcgactacct cggacttatc cgtccggcag gcagggtaga aaaccgtgtt caggcggtca
     4981 gcgaaattac gagagaattg aagcttatgg cgaaggatct tgcaattccc gttatcgttt
     5041 gcgcccagct ttccagaggc acggagggca ggggaggctc gcacagacct cagctttccg
     5101 accttcgtga atccggttct atcgagcagg atgccgatat cgttttaatg ctctaccgtc
     5161 cgaattatta cagcaatgac gaggagcagg acgatgacga tgaagcaaag ctttcgccga
     5221 acaaggcgga aataatcgtc gccaagaacc gtcacggtcc gacagacact gttgaaatgt
     5281 tttgggaccc cgatcataca ctttttatat ctgtggagaa gatcagaaat gatacgtgag
     5341 gtcaaaaacg ctgttgtaag atataatatg ccgctctccg gcaggacggt tgccgtcgga
     5401 gtatcgggcg gtgcggattc aatggcattg ctgcacgttc ttcttgagct gaaggatgaa
     5461 ttcggcatga aaatcattgc ctgccacgtc aaccacggta taagaggaga gacggcggac
     5521 agggacgaga aattcgttgt cgaagcctgt aaaaagctcg gcgttgaagc tcgtgttctc
     5581 agagtcgatg tgccgggaac ggcaaaaaaa atgcatctcg gcgttgagga atgcggtcgg
     5641 agaatcagat atgatttctt caattccgtt gccgacgatg ttattatcgc aacggctcac
     5701 acgctcagcg accgcagcga aactctgctt cttaatatca caaggggagc gtccgtcaaa
     5761 gggctttgct ctatccctgc tgtcaggggc aatatcgtca gaccgctcat cgactgcacg
     5821 agggcggata tagaaaagta ttgcagcgac aattccatag ccttcgttac tgacgagacg
     5881 aattttgagg atatttattc cagaaacaga ataagattaa acgttattcc cgagctgaaa
     5941 aagctcaacc cctcgcttga aaaagcgttt ttgcgcctga tttcaaacgc cgaggaggag
     6001 aatgatttca tggacggctt ttcccgggaa atactnnnnn nnnnnagtga ggctcgccga
     6061 aggctatgac gcaggacttt tgaataaaca gcaccgagca gtgagaaaac gtgtgatctt
     6121 tgaaataata aataccgaga cgggaattat ccccgaggcg gtgcacattg agcaggttga
     6181 cggaatcctt tcgggcggca gaaccgagat tatcggcagc actgtcgttg aggtcaaaaa
     6241 cggcattttg aaaatcaatc ccgagacgga aaattatccc gattgggaat gtgattttga
     6301 ccgtcttatt gcggaagctc ctttcggaaa aatcaaagga gaaataattc atagaaataa
     6361 tttaccgccg aaacagtctg ttcataataa agtgttagac tatggcagta tcgtaggtca
     6421 ttgtgtgctt cgcaacaggc ggccgggcga taaaatgaag ctcgcgggca gctcctgcac
     6481 caagacttta aaaaagcttt tcagcgagaa gcatatcgaa aaccgaaaca gcgcagccgt
     6541 ccttgcggac gaagtcggca tttgctgggt tcagggattg ggctgtgccg acagatgtaa
     6601 aataaaagcc gaaacagata aaatcttaat tatcggagag gacaaacaaa atgattgaga
     6661 atgacattaa agaagtatat ttcagcaagg aggagctgca cgggatcgtt aaaaggctcg
     6721 gcgagcagat aagcaaggat tacgagggta aaaaccttac gcttgtcagc atactcaaag
     6781 gctctgttat attcatggca gaccttatgc gtgagatcac cgtgccgtgt gagatcgact
     6841 ttatgtgcgt ttccagctac ggcaacggaa caaaatcctc cggaacggtc aagattatca
     6901 aggatcttga cattaacctt gaaggtaagg atcttctcct tgtcgaggat attctcgaca
     6961 gcggaaagac tctttattat atatcgcagg tgctcaagac gagaaacccg gcaagcatca
     7021 ggatttgtac tcttcttgac aagccggaga gacgtgaggc ggacatcaag gcggattatt
     7081 acggaacctt cgttcccgat gagttcgtag tcggctacgg tcttgacttt gcggaaaagt
     7141 acagaaatct tccgtatatc ggcgtgctca agccggaaat ttatgcggat taacagggag
     7201 tgaattattt gaacaacagt cctaatcaga ccccgaagca aaagaaaaat aaggtttttg
     7261 ccatattgcc gtatattctt attcctctga tgatggttat cggtatcgca atcgcatctc
     7321 agcagtcggg caaccgttcg gcggaggcta agtattacca gatcgttcag tattttgaca
     7381 aggatcaggt cagcgagttt acgctcaacc ttacgagcgg aacgctgaaa tacaagctca
     7441 agggcgacga cacaaaatgg cagtacaagg ttccgaacgt gtctgttttt gttgatgaca
     7501 tcaacgagac cgtcagggag cataacaaga atgccaagag cgaagacgac atgattaaat
     7561 atgagctgat ttccggctca tcgacctcat ggtttgtcaa tcttcttccg acgataatta
     7621 tgggcgtgct cctcgttatt cttcttgtca ttatgatgag acgaatgaac acaatgggcg
     7681 gcgacatgaa caagaccctg aacttcggca agataaatgc caaaaagcag caggatcaga
     7741 agtcaaagac aacctttgaa caggtcgcag gcgcagatga ggaaaaggaa gagctcagcg
     7801 aaatggttga cttcctcaag tgccccgaaa agttcagcac cctcggcgca agaatcccca
     7861 agggcgtgct tcttgtaggc cctccgggta caggtaagac cctccttgcg agagccgttg
     7921 ccggtgaagc aaatgttcct ttcttctcga tttccggttc cgatttcgtt gaaatgtatg
     7981 tcggtgtcgg cgcttcgaga gtcagagacc tttttgacca ggcgaaaaaa gacgctccgt
     8041 cggttatctt cattgatgaa atcgacgccg taggacgcca cagaggcgca ggaatgggcg
     8101 gcggacatga cgagagagag cagactctca accagcgcct tgttgagatg gacggctttg
     8161 agaagaatca gggagttatc gtcattgctg cgacaaaccg tcccgatatc cttgacccgg
     8221 ctctgctgag acccggccgt tttgaccgcc aggttagagt cggctatccc gatacaaagg
     8281 gcagagaggc tatcctcaag gtccattcca agaataaacc gctcgctccc gacgttgacc
     8341 tcagagttat tgcaaattcg accgtcggct ttgtcggtgc tgaccttgaa aatcttctta
     8401 atgaggctgc tttgcttgcc gcaagaagaa acaagaaggc tataacaatg gccgaaatcg
     8461 aggaagcgac cgttaaggtc atcgtaggca cggagaagaa gtcgcacaaa atgagcgaca
     8521 aggagaagaa gctcaccgct taccacgagg caggccatgc gatctcgacc tactaccttg
     8581 aaacacagga tcccgttcgt caggtttcaa tcattccgag aggctcggca ggcggctaca
     8641 ccatgtccgt gccgaccgag gataaatcat acaagaccaa gaacggtatg cttgacgata
     8701 tcgttgttct tctcggcgga cgagttgccg aaaagcttgt gctcggcgat atttcaacgg
     8761 gcgcctcaaa tgatatcgag cgtgcaacaa agcttgcaaa gagcatggtt accgagtacg
     8821 gcatgagcga caggataggc cccgtttcct attcttcggg cagcggcgag gttttcctcg
     8881 gcagggatta cggccatgcc aaggactatt cggaagcctt tgcgcaggag attgacgagg
     8941 agataagctc gattatccat aacggatacg accgtacgga gaaaattctc acggatcata
     9001 tggataagct ccatgagctt gcacagtacc tcatcaagta cgaaaagatt gacggcgaaa
     9061 agtttgctaa gcttatgaag ggcgagcttc aggaggatac cgaggagccg gaaaccctcc
     9121 cgaacgttga cgaggtcaag gaaacccccg atacggaaat aatcgaataa aataagtata
     9181 aagctgtgac gaattgcaat tgttcgccac agcttattta ttggtgcatt tgtgcgcgga
     9241 aataaaaccc cggttctacc gggggaaaga gaaagagatg agcgacaaaa aacggcgagc
     9301 taaaagcaag ctgaaaagta tagacataga agaaacctcc gtttacaatg aaatgcagca
     9361 tttgtatcaa tggacagcga tagacgaatg cacgagaatg aggtttgtgt acgggtttga
     9421 agaacacacg ccggagaact cggtaaagtt tcttttgatg gtgataaaag cgtttccttt
     9481 caggataaaa acaatacaaa cggacaacgg aacagagttc acatacaagt acataagtga
     9541 tgaaaatata tgtccgtttg ataaagcgtt acagaagctt ggaataaagc ataagctgat
     9601 accgccgaga acgccgtggc acaacggaaa ggtagaacgc agtcacagaa acgaccag
//
LOCUS       DAWBSW010000065         9348 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1526_length_9348_cov_2.656300, whole genome shotgun sequence.
ACCESSION   DAWBSW010000065 DAWBSW010000000
VERSION     DAWBSW010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_9348_cov_2.656300"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8902..8911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2059 a         2178 c         2380 g         2721 t
ORIGIN      
        1 cgtttgaggg gcgtgtggta atcccgtacg ggttcaagtc ccgtctctcg caccaaataa
       61 agaagagtcg caatagcggc tctttttatt tttcgcgggg cttgaacccg agagggtctg
      121 agcgtaaaaa aacagtcctg tggactgttt ttagcgagga gcggtgagcc gggtactgtc
      181 gctaagcgac ggtcggcgag ccatcagaat gcaaggcgca gccgcagaag acgccaagtc
      241 ccgtctctca caacaaagat atagccttga aacaacgtag ctacgtagtt tcaaggcttt
      301 tcttttgccc ctttggtgtt tattattttc cctttatttg aatagagtcg tacattagtg
      361 tgcttttttt gaaccaaacc acttagtctg tttcactgac gacgtttatc tttgacttgt
      421 tcgattcgct actatacatg gatgatgagg attggtttta cctgtccttg agttgatgca
      481 gacgccaagc ttgaaaatat agatacaaca aaagagctgc gacttcattc ggtcgcagct
      541 cttttggctg tttgctgtta tttcagaatt gcaagaagca atttgattag attgaaaagc
      601 tgttcgaaga atacaactac ccaattctgt tccgttggtt cttcggtcgg agtcttgtcc
      661 ggattttcgg tcggtgcgtc gtcgggcttt tctgtctcga ctgtctgctc gattatgctt
      721 atatattccg tataagccga gctcgtttca agctccgact gctgagcgtt ttgatttatg
      781 ctctgaagca taatgcctgc ctgctgaaga agctcagaat cagattcatt gatcactccg
      841 gcatggttgc agtcagccgc aaaccatgca gcttcgccga gctgttcctt tgtgaacatg
      901 ccgccgacaa tgcagttgac aagcgttgcg tccgttgcgt cgtaccagcc gtcgccgttt
      961 acgtcgccga agataacaag tgtgtaggtc ttgatgagat tgccgtcctc gtcgtaaagc
     1021 tcaatcttag tgcccgtgcc gttacccttt gaggtggggg agaacttgag cgaaccgctc
     1081 tctgcggcaa gatagctgtc aagagagtcc gtgtgcggct caattccgta aacaaagagc
     1141 ttctcctcgt caattacgac gccgtcctta acggccttta tcttggctgt gacgagcttc
     1201 tgaagcttgc cgagagcttc cgtgattctg tcagccattg cgttgatctc gttttgctga
     1261 gtgatgttga gcgtgtaatc aaccgcatcg acagccgcct tgactgcatt gtagctttcc
     1321 tcggtgtact ttgttgaatc gagtttattg aactctgcaa ccgccgcatt gactcgatcg
     1381 taattaccgc caaggtattc gagcgcatct gttgcgtctt tgagtgcggc gatagcgttg
     1441 ctgatgtcct cggtctttgc cgaagcgttg ttgtaaacag tctctgcgtc tgcgattgcc
     1501 tttgtgaggg cagccgccga ggcttccgta cgatttgaaa tgtcaatagc cttagcgtct
     1561 gtaattgctt tcagaagagc gtcctttgaa acggtgaccg ttaccgtaca ctgtgcgctg
     1621 tggccgccgt ccttggtgta agcagtgatg attgccgtgc cgccgtttac gaatgtaacc
     1681 gtgccgtttt caactgttgc aacgctcggg tcgctcgttg tccatacgac gtcggttatg
     1741 gtagctccgc cgactccgag ttttccctta ggctcaaccg aagcggtgag tgctgcgctc
     1801 tctccgggct tgccgctgag agacgactga ttgagagtaa cgccggttgc ctggatgttt
     1861 acaaatgaaa cgtatacata gtccgttacc gtgttgccga aatggtctgt tattgtaacg
     1921 gtaatctttg cggcacaggc cttgttctct ttcggagaaa ccttgccgtt ctgatcgact
     1981 tccatgaggt cgttatccga cgaccatgtg atcgactcgt acatagcgtc attaggcgca
     2041 tatgtatatg aaagctgaac gctctgcgaa ttgtaggttt tgtagatagg aacctttacg
     2101 gagatgaaat cgggtgcgtc ggaaccgtta tatttgattg taacgttctg agcacggtta
     2161 tacttgacga gtgcggaaac agcactgttg aggttagctg ccgccgtgtc aatctcactc
     2221 tgcgaagcgt ctgcgtcggc ataaaccgtc ttagcgcttt caagagccga tttgaatgtc
     2281 gcaaacgagt ccttggtgta attctgctcg ttgtagtttg cgctttcagc gtcgtcgata
     2341 gcctttttga ggactgtttt gtccgttgca accgttataa cgcaggttgc cgaaagtccg
     2401 ccgacaacgg ttgttgccgt aatctttgca acaccgccgc tgacaaccgt aacaagtccg
     2461 ttttcgtcaa ccgaagcgat ttcggggtga tcggattccc atgttacctt gttgagatcc
     2521 gcttcgttgc cgtctgcgtc cgtaaccgaa gcagtgagct gaactttttc gccgagagta
     2581 tgggttgtgt actcggacgg agtgacggtt attgcagttg cagggttctt tgtgaatata
     2641 attgtaaccg tcttggtgta aacattgccg aaatcatcgg tgagcttaac tgttgccttg
     2701 ccgtagcatg cgctgtttac actcggagcg gcaatgccgt tcatgttgac gaatatgtcg
     2761 ccttcatatg accactctgc cgaaacatac attgcgttgg cgggatcggt cgaaaaatcg
     2821 agctgtgccg ttgccatgat gtagctctga tctgcgggaa cctttacggt tataacgtcg
     2881 gagctgtcgc cgttatagga aatgtttacg ccggaaacgt agatataatc tgcgagtgca
     2941 tttctcttct cggtgagaag tgcgagagca ttgttcacct ctgtctgtgt cggatcctcg
     3001 gcgttgagtg tttctgttgc cgaggtgagg gcctttttga actctgccca tgttgcaggg
     3061 gtataggctg tttcaacgag tgcgtttgcg ctttcgaccg ctgcgctgag agccgatttg
     3121 tcggcataaa ccttgatgct tgtctgagca gtgaagccgc cgtcaagcgt cgttaccgta
     3181 attgtgcaag tgcctgtgtc cttgaaggtc aggttgccgt cattgtcaac ggtgcagacg
     3241 ctttcatcgc tcgatgacca gataacatcg gtgatgtttg cgccgcccat gccgagcgtt
     3301 ttcttaggaa gaacagttgc tgttatcttg tccgttgagc cgacggttgc gtttgcgatt
     3361 tccgtcttgt caacgctgac tcccgtaacc ggggtattgg caaaggaaac ataaacgctg
     3421 tcggaatatg ttcttccctt gtagtccgtt gccgtgacgg tgattttggc aaggcacgcc
     3481 ttgtttgccg tcggtgtaac tacgccgtcc tcaaccttga ccgattcgct gcttgatgac
     3541 catacgacag aatcaatcgt tgcgtcctcg ggggtgagct tgtagccgag ggtcacggac
     3601 tgttcggaat attttgaagt gatgggaacg ctgacagaaa catagcctgt tgtttcctct
     3661 ccgtccttgg tgattgtgat attttcaagc ttaataaatc ttacaagcga attataagcg
     3721 ttgttaagct cctcgtctgc cgcattgata actctctgct tggggttatc ctgcgcaatg
     3781 atttcctcgg cctttgccat tgcggcttca agaaccgccc aactgtcctc ggtgtagtcc
     3841 tctgaaatga ggttaagacc gactatcatt gcccttgttc ttataagagc tgttttatcg
     3901 gatcttacgg ttaccttaca ggttgccgaa acaccgccgt caacggaggt tgccgtaatc
     3961 gtacatgtgc cggcttcgac gaatgtgaca gttccgttct cgaccgttgc aacgtcgggg
     4021 tcagaggttg accagataac gtcgccgacg ctcggcttgt cgccgatagt tttgtccgcc
     4081 gttaccgtcg cggtgagtgt ctgcggctcc gaaccgtagc ctacttcaat ttccgtttta
     4141 tcaagcgtaa cctttgaaac aggggcgttt gcgaacgaaa cgtaaacgct gtttgttaac
     4201 gtgttgccgt aatggtcggt tgcggtcaag gtgatttttc ctccgcaagc cttgttggag
     4261 gcaggggaga ccttgccatc ctcgctgacc ttgatgccgt cgttgtctga tgaccaaaca
     4321 atgcttgagt acatagcgtc ggccggtgca aagtcgtagg tgagctgaac gctctgcgag
     4381 gtgtatgtct tatagatagg aacctttatc gcaatgaact gcggtgccgt ttcaccgttg
     4441 tagtaaacat taagcttctc catgtggata tacggcttaa gtccgtcgag tgcattgtta
     4501 agtctgagcg ttgcggcgtc gatttcctcc tgggagtatt tagcgcccgc ttcaacctcc
     4561 tttgcgtgct cataagctgc cgtatattcc gtgcttgtct cgtatgtgtg ctcctgaatg
     4621 tcaacctgag cttcatcggc tcttgcgatt gcggcacgaa gcgctgtctt gtcaccgccg
     4681 atagttacaa ggcaggttgc ctgaataccg ccgtcacatg aggtggcggt gacagttgcc
     4741 tggcctgcat caatataggt cagttgaccg ttttcgtcaa ccgttacaac gcttgtattg
     4801 tcggaggtcc aaataacgtc ctgaatcgtt gcgtcatatg tgatgataga accgacgtcc
     4861 ttgcattttg cgctgagctt taccggctcc gaatcgaacg gagcgttgac ctcggacgga
     4921 gtaatttcaa tgcttgtaac gggatatctt gcgaatgaaa cgttaacctt gtcagtaacg
     4981 ctgttgcctc gctcgtccgt gacctttatc gtgataacgc cgtagcaagc cttgttgacg
     5041 gtcggggaga ccgttccgct ttcgtcaacc gcgatatccg atgtgctcga tgtccattca
     5101 acagttttgt acattgaatc aagcggactg attcttgtat caagcttgat tgaattgttc
     5161 tgatatgtga atgatgtcga aagatttact tcttttgaga tatattcgcc ggcatcctcg
     5221 ccgttatact gaatataaac agcctgcggc ttgatgagct ttttgagcga attaaacgct
     5281 gcgtcgagcg aagcggttat tgcgtcgata tccgactgct ctgcgttctt atcattatat
     5341 atctcgttag ctcttgcata ggcttccttg agtgcgtcat aggaagcgac cgtgtaatct
     5401 ccttcaacaa gggcggcatt tgttaccttg ctgatttcct cggcaagggc gtccttgttc
     5461 gtcgttacgg ttaccttaca ttcggcagtg tgaccgccgt caaccgttgt taccgtgatt
     5521 acagcttcac cgacggattt gaaggtgagg ctgccgttat cgaccgatac aacatcggga
     5581 ttgcttgatg accagataac cgattcaatg cttgcgccga tcatcggagt gcctgtcggc
     5641 tgaaccgtcg ctttgattga tgccgtgtta taaaccttgc cggttatttc gctctcgctg
     5701 agagtgactc ctgttgccgg gacattcgca aagcttacat aaacgctgtc ggaaacggag
     5761 cagccggtgt aatccgttgc cttaacggtg atcttcgcac tgtctgcgga gttgtgagtg
     5821 ttggttacaa gaccgttctg atcgactgtt accttgtcgt tatccgacga ccaaacgatt
     5881 gacgaataca tcgcattgct cgggttgaga gccgcactga gctgaaccgt cttgttctta
     5941 tatgactggt aaaattcaac ctttgtagtt atgtgcttcg gtgcaggctc accgtcacgg
     6001 gtgatcgtga tgccgtatac ctgaacataa gcaccgatgc gtgaatagat atcaataata
     6061 ttctgcgccg ccttgttgca ggtatcctga gaaaccgtat cggtgttctt gacctcaact
     6121 gcggttttgt atgcgtcctg caatgctgcc caatcctctg ccgagcagtt atcttcctta
     6181 acattgcctg tttcaacaag gtttatcgca tagttaagag ctgttttgtc agcctttacg
     6241 ttaacagtac agcttgcggc aaatccgccg tcaacagttg ttgccttgat gatggttgta
     6301 ccgaagtcaa cgggggttac aacgccgccg tcaaccgtgg caaccgtcgg atttgaagaa
     6361 gtccagataa tgtccttgat cgaggcgtcg ccgactccgg cagtacccgt cggagcaacg
     6421 gttgctttaa ttgtttcgcc tgtgcctaca acaaggtcaa gcttgtcttt gtcaagtgta
     6481 attcctgtta cgggatagtt tgcaaaggaa accgtacagg tgtcggtgta gccgccgtca
     6541 taggtcttga cctgaacgac accgtatgcc gccgaatagc ccttgggact gacaacaccc
     6601 tctgcgttta cctcaatatt gcccgagaga accgaccagg caacgttttt gttggttaca
     6661 tcatagggaa gaatcgaagc ggtgagtgtg gtttttgcgt tcttatatgt gctgtagagc
     6721 ggaacatgga cgctgacaaa tttgccctgg tcaatcgtaa cgccctttgc cagtctgccg
     6781 atataaacct gatatgttgt tataacgtga gtgataaggc cggccttggc atagagagtt
     6841 accgttccgc cgtttgtgcc tgtcagcacg ccgtccttgg taagcgttgc aatgctgttg
     6901 gaaacgtcac cgtttgtcgc ataggttact gtttcaccgt cgtcaccgag gagaccccat
     6961 gttatatccg cctttgatct tgtcggataa agagaagccg tgtattgctg aggtgtgtta
     7021 agctccagct cctgcggtcc cgaaaccgca atgtcaacgg gagttgtgat aagtttacct
     7081 ttaacggtga attcaaggct ttgggaaatg ccgtcaaccg tagcggtaac cgttgcggtt
     7141 ctcttattcg ctgaggaaat tcctcctgcg tctctcgcct ttacgatgcc gttgttatca
     7201 atggtgagaa cgctctcatc ggaggagctc cagagaacat tctgcgagtt tgagcctgcc
     7261 ggaacgatgt ccgtcagcct taccattgtg ctgtcgccct cggtcagctc ggttttgtcc
     7321 gccgaaaggc tgaatttttc aatttcaagg ctctgaacta ttgtaaatgt aatcgaatcg
     7381 tttatagatg ctttcaaaag atagtttgaa agagtcttga ccgctgttgc gataaggtct
     7441 gacgtattgc ctgttgcgcc tgcgatcgtt acaagaacat caagaacagc cttgagtgcc
     7501 gattcgctga tgttaagtcc gagaagcttg atcataattc ttgcaagctg agcggtgtca
     7561 accttttcac ctgcggcaat tgccgagttt atgtagctca tcaatccgtc gacgaggtca
     7621 tcggcattcg gcgaaacctt tacagtgacc ttgccctctt tgagaaaggt gacgttgccc
     7681 ttatcgtcta tcgttgcata atccttgccc gatgtccaga tgctgtcggt gtcaatgctc
     7741 catgtaacgc cgtagccgag acgcagggga gtgacgccgc cctcaagctt gacctgtccg
     7801 ccgaccgcaa ccgtggtggg aacagattct ttatttgtga tgaatgctcc gttagggata
     7861 accttggagt accatttgtc cgaattggta acgagcactc ttacctgatc cgaggcaaga
     7921 acttcgccgt ctgcggaata aagggtgacg gttatacctg tatcaagcga attgagcttg
     7981 tcctcaagct gctttataag gtaatcggag atattgctcg gtatgccctt catagatgcg
     8041 cgaaccgttt caacaatggc ttccgtatcc attgtgtcaa tgtcgatttt gtcattgaac
     8101 agaagctttt ccatggcctt tgccagagcc ggaccgatga ttgcgacggt tcttacgtcg
     8161 ttgtcgatcc acagtctgac ggcagcgccc ttggagctgt catgtgcata tatttttcct
     8221 gttgagtcaa cggaaacaat gagaggagcc tcacttgtcc actttatgta accgccgtcc
     8281 ggcatagaac agtcaagcag cttatgtgtc agttggagct gttcgctttc cataagggag
     8341 atctgctccg tgactgatgt gccgtcctct cggcaaatgt cgattgcatg ggtagccgcc
     8401 gctgaagctg ttgttccgaa agcgggaaca atgactgcga cgagcattgc catcacgaga
     8461 atcaggcttt gccatttgat gattttcttt ttcatataaa tacctccaat cgggatttat
     8521 aattttacat tctattttat cacattgcat atgcgaatat tttctaaaaa agaatatata
     8581 ttttatttta tataatttgt atgcagaatt taaaaattaa atatttttta ttattctaat
     8641 gttattttaa taattgtaaa ctatacttta gtcactgaag caaatcagaa atgaattttg
     8701 gaggtaatga aacatgaaaa acattaagaa aatcatctcg gtagttatgg cgtgcctgat
     8761 tcttgcaggt gtgttcgcaa caagctcctt cgcggcgggc tatatcggca aggacaaggc
     8821 aaaggaaatt gcacttaatc acgcaggcgt tacggcagac aaggtatcgt ttatgaaatg
     8881 tgaatttgac cgtgacgacg gnnnnnnnnn ncgacggcat tgccgtttac gatgttgagt
     8941 tctattttga cagatgcgag tacgattacg agatcaatgc aaagaacgga aagattctca
     9001 aggttgagaa ggacaccttc ggcgttgata tcccgactcc cgataaggag tacatcacaa
     9061 aggcagaggc taaagcaatc gcttttgagc acgcaggcgt aaaggaagca gatgcgaaga
     9121 aggttagagt agagtttgac tttgacgacg gtgttgcgga gtacagcgtt gaattccatg
     9181 ccgacaagtt cgattatgat tacgagatcg acgcagtatc cggcaagatt ctcaaggttg
     9241 aaaaggacag agattttgat ttcagcgacg ttttctttgt tcactggttt gacgctatcg
     9301 tatcattcct taaaaatctt tttaaataag atataagttc aataagcc
//
LOCUS       DAWBSW010000066         9297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1537_length_9297_cov_2.966566, whole genome shotgun sequence.
ACCESSION   DAWBSW010000066 DAWBSW010000000
VERSION     DAWBSW010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_9297_cov_2.966566"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3918..3927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7675..7684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2658 a         2087 c         2081 g         2451 t
ORIGIN      
        1 gtattgcagt tctatccgtg cattttcttt tttctttatc cagtagcatg cacggtaaat
       61 atcaaccatc gggtgttcaa gaattgtctt gcagtcaacc ctgtttagca gatcgattat
      121 tctgcgcata gctttctcga atttttcgcc ctcataatga taaggataga gaatatcgtc
      181 gctgagacgc cagttgatgt cattgacaag catcgcctga aaatagggcg gaaccttatc
      241 cggaaatgtt gcgaaaagag tttcaaaata ctccattgat gtctcaaaaa gataaaaggc
      301 atggaagtac attttcatta tgcttgtttc gttgccctta ttatacatgt atgcgccgtg
      361 agagcaatag cccatcttca tcttgggcat gagcacacgg ttacaatatt cctgatcctc
      421 gagtttaaag ccgggagttg tattgaaaag aacattctta tcgccgagat tttttacgca
      481 gacgttgact cttgtctgtg tcagataggg gtatttctcc aaatcatata cgccatcgtg
      541 cttaagcatt tcaaaacgga aatgaggttt aagaataaca tcgtctctga aacgctcctc
      601 attaaatgta acaaggtcaa cctgatcata taccttatcg aaaaaacgag ttactttttt
      661 cactgtttcg ggagtaaaat aatcatcgct gtccagaaag aaaagatatt ttcccttggc
      721 gtgctttatt ccgaaatttc tcgttgcgga aagtccctcg ttctcttttg aaaagatttt
      781 aataaagtca tatttcttgc tgtactcaag gcagatatcc cagctgttgt cgggagaacc
      841 gtcatttatg acaagaacct ccatttttga tttatccatt gtctggttaa ccaaagagtc
      901 gagacaaatt ctgagatact gctcaacatt atataccggc acgataacag atgcctgata
      961 ctcaaaatcc atataatcac ctttcaaatc agttacgatt agtataacac attcgataca
     1021 agtttgcaac agcatattta aaagcgtaaa aagcttattc gaaaataggt gaaattttta
     1081 acaaaattta tgcttaacta cttccatttt ttataatgac gtgttataat taaacggaaa
     1141 ctgattataa atcaattcag gaggtaaaat atggatcaga aattagaacc tttatttacg
     1201 ccgtggaaga tcggtaattg cgaaatcaag aaccgtatag ttctgacgag catgggcggc
     1261 accgaccttt tcggatggat ggaaaagaac catttcgata aagacggcgc aaagttcatt
     1321 atggaaatcg ccaagaacaa cgtcggactt attctccccg gctgtcagcc cgtttataac
     1381 ccgatgttcg gtcagtggct gtacaagaac aagaagatgt acgatgatct caaaaagtgg
     1441 atgcccgagt tccacaagac gggcgcaaag ctttttgttc agcttaccgc aggcttcggc
     1501 cgttcgttca caatcagctc catgatggag acccttttca ccaatcctgt gcttaaggtt
     1561 ctcagcaagc cttttatgga tcttgatatg atcacggctt cggcaagccc ctctccgaac
     1621 cgctggtcgg ataaggttcc gtcgagagcc ttgactaagg aggaaatcaa gaagtttgtt
     1681 gacgcattcg caaagagcgc aaagatgctc aaggacgcag gcgttgacgg tgttgaggtt
     1741 cacgctgtgc atgagggtta tcttcttgac cagttcacac tcaagtatgt caacaagcgt
     1801 accgacgaat acggcggaag cttcgagaac cgctatcgtt tccctgttga ggttgttcag
     1861 gccatcaaga aggcttgcgg cgacgatttc ccggtttcac ttcgttacag cgttatctcc
     1921 aagacaaagg gcttccgtca gggtgctatc cccggcgagg actatgttga ggtcggccgt
     1981 gacatggaag aatccgagaa ggcagcaaag tatcttcagg atgcaggcta cgactgcctt
     2041 aactgcgata acggtacata tgacgcatgg tactgggcac atcctcctgt ttacatgccc
     2101 gagaactgca accttgcaga tgttgagcac ataaagaact ttattgatat ccccgttatc
     2161 tgcgcaggac gtctcgaccc gagagttgcc gcagactcga tcaaggaagg caagcttgac
     2221 ggcgcaggct ttgcacgtcc gttccttgca gaccaggcat gggtaaccaa gatgatgaac
     2281 gatcaggagg aggatatccg tccctgtatt ctttgccaca acggctgctt caatatgtgc
     2341 cattacaagg gcgtaccgaa cgatcaggag ctttccgaca gtctccacct tgcacgctgt
     2401 gcggttaatg cagagacaat gcagtggaac aagcattata ttaagaaaac gacttcgccc
     2461 aagaccgttc acatcatcgg cggcggtatc ggaggcatgg aggccgcaag agttctcaag
     2521 ctcagaggcc acaacccgat catccacgag aagagcgatg ttctcggcgg tacatttatc
     2581 cccgccagcg cagagagcta caagggcaag cttcgtgagc ttcttgagtg gtacagactt
     2641 cagatgaaga agctcaacat tgaggttaag ctcaacgacg aggtcaagga cattgccgta
     2701 ttcggcgacg caccggtaat cgttgctaca ggttcaactc cgcgtcagct cagaagagtt
     2761 ccgggctacg agaagatggt tgaggcttgc gaattcctca caggcacacc cgtaggcgat
     2821 acggttgccg ttatcggcgg cggacttacc ggcagtgaga tcgcttatga gcttgctctc
     2881 aagggcaaga atccgatcat cgttgagatg aaggacgacc ttgtaagcca gaagggcgtt
     2941 tgtcttgcaa actcctcata cctcagagag tggttcgcac tcaataagac tcctgtttat
     3001 cttgagacaa ccctcaagga agttaaggac ggctcaatcg tttgcactca gaaggacggt
     3061 aaggagattg aaatcccctg cgattccgtc atcagctgtg caggctacat tccggctcct
     3121 cttgccgaga agtcgggcaa cgttcacctt gtcggtgact gtcttgcaat cggtaacctt
     3181 cgttccgttg tatggcgtgc atacgacgtt gcaatgaaga tctgagaaaa gtaaaaaaat
     3241 taagaagcag tcactcggct gcttcttttt cttttcttca aaacggcaaa tcgccgtcaa
     3301 cgtctttgta ctcataattc atcggcagtt cctcggtcgg agaatagctc gaatcacaat
     3361 attccgggaa gcaggcaagg aaccaatcac attcatcgca acagatttca tacacatcat
     3421 tgcttccgtt gccctcacag gtttgcggtt tgccgggagt taagacagcg ccgctgctgt
     3481 ccgaaacggt ctccggtatt ttctcctcgg cttccgaaac aaacttatgc aagtcaaaat
     3541 tcattgcctt ttcctcccat aaaacaaaaa atgcgcagcc gaagctacgc actaaaaacg
     3601 atgcttcgat cttgtcgcaa aacagttcat agccacagga tatgatgatc ttagcataca
     3661 tttttaacaa atgtatgcct gtggcattaa taatttactt ttgaactgtt ttgcaccgac
     3721 attatatcac acgtcatgat tgctttcaat atatttttat aaattactca aaaaatagaa
     3781 ggcattttgg ctctgcttta gagaagcggc gaaaatatat ttcttctgcc cttggccgtt
     3841 gccaacaccg catctttgaa attctgctga aatttatttt tagcgtggaa gtctacttgc
     3901 ggtatcaagt accaaaannn nnnnnnnttt cgcaattctt tcaatgcttt tttctcgatt
     3961 ctcgaaacat atgaccgtga aatattcagt tttttggaaa cctcgagctg agtcatgggg
     4021 cgcataccgt caagaccgta acgcatcatt ataattgatt tctctctcgg gtcttttatt
     4081 ctgccgacct cggttgaaag ctttttcagc atagttattt tataaatatc ctcaacaatt
     4141 tcatcttcgg tggagataat atccataagc gtcagcggat ttccctcgct gtcggtatca
     4201 atcggctcgt tgactgaaat gtcctgagcc gtttttttct gtgcacggaa ctgcataaga
     4261 atctcatttt caatacatct tgccgcatag gttgcaaggc gtgttccctt gtcgggatca
     4321 aatgtattta ttgccttaat cagcccgatc gtgcctatcg agatcaggtc gtcctgctga
     4381 acgctgctcg aataatactt tttgataata tgtgcaacca gcctgagatt atgctcgata
     4441 agctcgttct ttgcatttac atcaccgttt ttcattgcca gcagacattc cctctctctc
     4501 ttggcggaaa ggggaggagg aaaggagccg gtgccggaga gatgaagcgc aaaaaagaaa
     4561 agatttgata ttgttcttat aagcaattca gctatcattt aatcaccgtc cgttataatt
     4621 cttatgaagc aaatgcttaa ttgataacgg attgatttgt atatttatat aaaaaaaata
     4681 atcggctatt gttcaaaatg taaaattagg atatattgta cgttattata gttgactttt
     4741 gatcgaatta aaagtataat aacaatgtat aaaagtgtgg tattgtatgc actttgtata
     4801 gcattatttt ttgggaggaa tattatatgg ctaaaagcag acgtattctc agcgtgttgc
     4861 tggctatcct gatggttctt actatcgttc cggcaggatt gatcggcacc gccggtgccg
     4921 tttcggatga tgagatcggc acatcgtcgg ttgagcttag tcagagcttc cttgagaggc
     4981 ccgatattac aatcagctca acggcagtca ttcgtacagc cgccgaagca aattcacagg
     5041 ctgcaggcac aactattgta aaagctacgg cttcgggctt gcccgagctt acaggtgata
     5101 aggcggaagc cttctatgca ggtgagaccc cgcaggatcc gatggtagtt ttcaccacaa
     5161 acaaggcttt gaaggctaac ccgacaatca catgttcaaa tgcaaacctt accttcaccg
     5221 cagcatcggc aggtgcatat gctgacggtg tttacaccta ttcttggaag gttgcaggtt
     5281 ccagcatttc cgtgaagcag ctcaactttg aagtcggcta tacatatgag tacaccgatg
     5341 caaacggcaa ggtcgtaaca aagacctata cagcttatac cacaagtgcg gttgagaacg
     5401 ttgctcagcc cggcggtatc tcggcttacc gttaccgtca gaatcagttt tttgatcata
     5461 actgtaccaa taaagaaaag gttgaatata ccgctcgtat agtaggtgca aatactttcg
     5521 gtacatatgt cgatacaact cagaccggct ggtcaagagg atattgggat ttcaataccg
     5581 gcagctttac cacagtcagc ggtacagcgg caggctacgg tcaagttaga tttgacggca
     5641 aatcggtcgg cggaactcag aatgcaaata cagccattga cgttaaccgt cctgttacgg
     5701 tggcgtatgt tgacagaagt atggttgctc tgagcaacta taacctcaga ttcacaatta
     5761 ccgattttct caacagaaca gacaaatata gtgaatacgg tattaaggaa ttcaagctcc
     5821 tcacgggttc atctacattt gacggaaatg aagcaagtag caataccgca gaagcacagc
     5881 ttgttcccgt caagcctgcc gagtataatg aaagtacaaa ggaatatgac acagttgcag
     5941 gcggaacgat caccattccg tttacgggta caacctatga aaagaatgca acagcacttg
     6001 ataacggaaa taaaagtgta gattacacaa tcgttgtttc tttcggtgat acatataagc
     6061 acgtttcaag aattcgttca ggtatcaacc tcaacatcgt tacatacgat aagtccagcc
     6121 tccgttcaca gcttcaggat ctttacagac tttatactcc gaccaagctc atggcttccg
     6181 agtcggagat aggcaacagc ccgaactcat ggttctattc tgagggttgg gatcagtacc
     6241 ttgcagctat gagaaacgct cagtatattc tcaatgaccc cgacacggat cagcagaaga
     6301 ttgatgccgc aacagaggct ttgagatctg ccgttgcagc tcttaaggtt gctacagccg
     6361 attatacaga ggctaacgta gttaaggatc aggttagggc tcttgattcg tcaatttata
     6421 cggatgaatc atggtcaaga gtacagactg ctgtttccaa cattacagac ggtatcagcg
     6481 ttttctgcca gacttatctt gattctcttg ttaatgatct tcgcgacgct atcgccaaac
     6541 tcgaaaagtc tccggcaaat tacgatgaag ttaacgacat ggttagaatc tatgagggtc
     6601 ttaatcccga gcattacacg gctgaatcgt ggaagattct ccgcgacacc gtaaattcgg
     6661 ttgactatac tctcacagtc gaaaatcagt cgcttgttga cgcttatgca agaagtattg
     6721 acagcgctat ccaaagcctt attctcgccc cggcagatta taccgaggtc aataatcagg
     6781 ttactcgcta caatgaagtt cttcccgaga aggatctgta caccgatctt tcatggagag
     6841 ctgttcaggc ttcctacaac gcaattgagt gggacaagaa ctggcagcag caggaggacg
     6901 ttgacgatat ggcaacgaac ctcaaaaatg ccatcggcag acttaaatat aaggatgctg
     6961 atcttaccgc tctcaatgca gctataaagg cagctaacgc cgagaaggaa aactggtata
     7021 caccggattc atgggcaccg tttgcccagg ctcttgcagc ggcaaaagag gctgcaaacg
     7081 ctactccggt atacaatatc aagcagcaat caataattga cggtctcaag gaaactcttg
     7141 tacagaaaca gggtgagctt gttccggccg acggcgatta cagagaagta gatgccgcta
     7201 tctctgcagc aaacgagctt gacgaaagca gatatacggc tgagtcatgg ggaaatctcc
     7261 agaatgctat aaacagtgtt gtatataatc ttccggctaa ggagcaggaa agaattgacg
     7321 gttttgcaga tgcaatcaat aaggctatac ttgctcttaa agagattggc gcagactaca
     7381 cggcagtaag agcaatcatt gccgagtggg acgctatgga taagtcactt tatacagatg
     7441 catctatcct taacgtagca aataccgtta attcggttga ctggaatctc ccggtaagca
     7501 agcaggctac ggttgacaat tatgtagttg aaatccgtaa tgctatgaac gctcttgcgt
     7561 ataagccggc agattactct gccgttgacg cagcaatgct taaggttgaa gaagccaaga
     7621 acattcaggc ggcttttgca accgctcata acggatattc atactacacc cagannnnnn
     7681 nnnnccagaa gtcctatgac gatctccttg caacaacaaa ttacgacaag actcttgata
     7741 tcagatatca gtcaactgtt gacggctatg caaaggctat aaatacagct cttttggctc
     7801 tgacgccgaa cgcagcagat tattctgcgg ttgaagcagc attggcaaag attccggcag
     7861 actttgagga cggtgtttat accgacgaga cggctgctga ggttttggct gctaagcttg
     7921 ccattgacag aacgctttca acaaagcagc agagcactgt agacggatac gcacaggata
     7981 tcgagactgc aatcgcaggt cttaaatata agagtgccga ttacacaaag gttgaggcag
     8041 aaaaagctct tatcccgaag gatctcactc cttacaccaa gtcatccgtt gacaatcttc
     8101 aaagagttct tgaaggtata aactataatc ttgatatcaa ccatcagtca gaggttgata
     8161 attatgcaac tgccatcaag gccgcaagag aagctctcga gcttgacctt gccgactaca
     8221 caaaggtaaa cagctccaag gcaaaggctg aggaagctat caaggataca aattacacag
     8281 acgaaagcat caaggcagtc cgggacgcta tttcaaatgt tgttgagggt ctcccgaagg
     8341 ctcagcaggc gatcgttgac ggttatgcat ccgcaattga cgatgcggtt tcacgcctga
     8401 cggataagcc gctcgatctt acaagctata ataaagcact tgaaggcgtt ccggcaaatc
     8461 tcggcaacta tacagatgat tctgtaaagc tttataccga cgctcttacg gctgccgatg
     8521 cctacaagct tacaaacaac tcaatcagaa atcagacaga gtttcaaaca cttgtttctg
     8581 cccttgatgc agctattagg ggactcaagc tcaagggtgc tgattattcg gcagtcatag
     8641 cagcaaagac ggctaaggat gagctttaca aatccggatt gtacaagatt gcttcactca
     8701 cagcttatga tgagcttatc gcttcaatca actggaacct ctcaatagag gagcaggcaa
     8761 ctgttgacgg ttatgcaaag gctatcaatg agtttgtttt cgagctcaag aatgccgatt
     8821 acacagcact tgatacggct attgaagcaa agagaacaga gattgcaaag ggtctcttca
     8881 cagacgcttc cgttgcaggc tttgacgctc ttgtaaacgg cttcgaccgt acacttacaa
     8941 ttgacaagca gagcacggtt aacggctatg ttgaaagagt aaatgcatat acatttacat
     9001 acaagccggc tgactataca aatcttgacg ctctcattgc agaggttgag gctcttgatt
     9061 catcgcttta cacaaactat gatgaaatct acacaggcta catcctggac tacatcctct
     9121 cttacatccc gtctcacagg gattacaaca tcacggagca ggataaggta aacgagatgc
     9181 aggcaactct ccagagctat gtcgatatgc tcattctcaa ggatacaaag gtagcaaagt
     9241 ttgagcttaa gaacggagct aagtacaaga cctcaggcgg agtaacatac ataatcg
//
LOCUS       DAWBSW010000067         9241 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1547_length_9241_cov_2.735902, whole genome shotgun sequence.
ACCESSION   DAWBSW010000067 DAWBSW010000000
VERSION     DAWBSW010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_9241_cov_2.735902"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    286..295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    976..985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1238..1247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1900..1909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7075..7084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8899..8908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2640 a         1920 c         1814 g         2807 t
ORIGIN      
        1 tttgtatata atgtttcggc atttgataat gacaacgatg attataattt tacaaacctt
       61 attgtctttg caaaatttaa tgatgaagag gaatttattg accgtgatta cggtgaaacc
      121 gttagaaaga ttaccgacaa ttcatacaat accgccgaat ataatgtctc tgattatttt
      181 tattctgttt cttccggtaa agttaagatg aaaagtttat ttcttttcga taatggcggt
      241 tctttaacgc ttagcagaaa aagagcttac tatgcttctc aaagcnnnnn nnnnngagca
      301 agccgaacgt ctgtatcaat taaaagaaga ttggtcacaa gcaataaata atttggtaaa
      361 aaaagaaatt acagattacg acggaaaaga aaaatataat ttagatgagc ttgataaaaa
      421 taacgatgga tacattgatt taattacgat aatttataag ccaaccgaac aaaccaacat
      481 ttccgttgaa tgggcatctc ctttatggaa ttatcgtgat caaacaaatc ttgttgaaat
      541 tcaaaccgac aagaaaacta ttcaaagccg tgcgtatgtt caaatgacga aggattataa
      601 atatctttat accgatgcaa agggaaataa aataactgct atcggtgtta actgtcacga
      661 gacggctcat gcattcggcc tttacgatct ttatcagtcg tcgtatcaac cggtcgggta
      721 tatgtcagca atgggtaagc atacaaccga ggttgcgcaa tttatttcag taaaagaacg
      781 tgaagcactt ggttggataa ataataagaa tatttatcac attttaaatg aaggtgatta
      841 cactttaaaa cctgtaattg acgggcttac cgataatgta atcgggtata aaatggatat
      901 ccctgaaata aataaaacct tgtatcttga atacagaaga tttgacggaa aggggagcaa
      961 atacgattca caaaannnnn nnnnntgcaa acgggtcaaa aatcaaaggg ctttctttaa
     1021 aaagcggatt ggtttgctat ttattaaaaa ccggtataaa atttccgaat aatatgcagt
     1081 caagtgttaa taattggaat tacagtgttg cgggcggtca atataataat aagcccgatg
     1141 ccgcagtatc tctcggagaa gaaattgaaa taacagacgg actttatata aatgttacag
     1201 aaatgaccga gaatgaattg acgtttcata tagaaggnnn nnnnnnngga aatttcttca
     1261 attcatcaag cctaccttct gtaaacggag ttgaaattac aacttttccg gataaaatct
     1321 gtgtgggaga gatctataat tttaatgcaa atgttttagg aaaaaataat ccgcctcaga
     1381 cgattatttg gagcgttgaa aacaataccg atgataaaac tacgataaat gaaaacggaa
     1441 aactttttat cggcgaaaat gaaaaatcaa atgaaataac agtaattgca tcatatgaaa
     1501 ataaattctc agacaagaaa attatttcaa ttgaaagaaa atcgcatgat tttgaatatc
     1561 ataaagccgt aaaagcaacg tgtgaaaccg acggcaattt tgaatattat cattgccgtg
     1621 aatgcggaaa gtatttcgtt gatatgggcg aaacttttga agaaaccaca attgaaaata
     1681 tgattataaa aaagacagga catattcccg gagaatggaa agttgttaaa ccggcaacct
     1741 ctgaagaaaa cggaattatg gagcaaagat gtgtaggctg caataaactg atggcttcaa
     1801 aagaatacgg tttttatcct gaaaacccga aagaaaatgt aaagtctttt cttagtaatt
     1861 ttaaaaatct tataaaaaag ttttttgaaa ttataaaaan nnnnnnnnnt taatcggaag
     1921 ataaaaaaat cgacacgact ttttgccgtg tcgatttaaa tttattatct gattatgcct
     1981 tgcctgcgca gccgagaact gtgttgatct tgtgctcaac cattccctca attgcgtcac
     2041 gagccggtct gagatactga cgaggatcaa aatcgctcgg atgctcaaca aaatgctttc
     2101 tgatagctgc ggtcattgca agacgaaggt ctgagtcgat gttgatcttg caaactgcca
     2161 ttgttgctgc ctgacggagc atatcctcgg ggataccgat agcgtccggc atgtttccac
     2221 cgtacttgtt gatttcctca acaaactcgg ggataacaga agatgcaccg tggagaacga
     2281 tcgggaaacc gggaagtctc tttgatactt cttcaagaat gtcaaaacga agctgcggct
     2341 tctgaccggg cttaaacttg tatgcaccgt ggcttgtgcc gatagcaata gcaagcgaat
     2401 caacacctgt gcgctttaca aaatcctcaa cctgatcggg atttgtatat gaagcgtcct
     2461 cagcgttaac gttgacttca tcctcaacac ctgcgagctg gccgagctca ccctcaacaa
     2521 caacaccgtg agcgtgtgca tagtcaacta ccttttttgt aagagcaacg ttttcctcat
     2581 agggaagaga agagccgtcg atcataacag atgtaaatcc gccgtcgatg caagccttgc
     2641 aggtctcaaa atccggaccg tggtcaaggt gaagggcaat cggaagaccc gattcctcaa
     2701 cagcagcctc aacgagcttt acaagataga ggtgatttgc ataagcacgg gcacccttgg
     2761 aaacctggag aataagggga gcgttgagct tctttcctgc tgcaacgatg ccttgaatga
     2821 tttccatgtt gtttacgttg aaagcgccga cggcatagcc gccttcataa gcgtctttga
     2881 acatttttgt ggttgttaca agtggcataa aaaacactcc tttgttttat tacaggataa
     2941 ttatatcatt tttatccttt ttgtcaatgt gaaattgata taaaattaac gttcttaaaa
     3001 aggatttaga aactgtttat aagacgttaa ttatttgata atatctgtac ccatatacgg
     3061 aacaagaacc tcgggaatcg taactgttcc gtcctcattc tggtagtttt cgaggattgc
     3121 tgcggtggtt cttccgatag ctacacccga gccgttgaga gtatgaacaa gctgcgcctt
     3181 gctcttgggg tcttttttaa atctgatagc agcacgtcga gcctggaagt cctcaaagtt
     3241 ggagcatgag gaaatctcaa catatctgtt gtaggacggc atccaaactt ctatatcata
     3301 tgtctttgcc gatgaaaaac cgatatcacc cgttgaaaga caaacaacac gataaggaag
     3361 accgagaagc tgaagaactc tttctgcatc gttcgtaagc ttttcaagct catcataaga
     3421 agtttcgggg tcgacgaatt taacaagctc aaccttgttg aactggtgct gacggataag
     3481 acctcttgta tcacggcctg cagagcccgc ctccgcacgg aagcaagccg agtaagcgca
     3541 atatttaatc ggaagcttgc tgccgtcgag aatctcatct ctgtgcatat tcgttacggg
     3601 aacctcggct gtcggaataa gaaatagatc gtcggttgtt ttgaaagcat cgtcctcaaa
     3661 tttcggaagc tgaccggtac ctgtcattga agctctgttt acaaggaacg gaggaagaat
     3721 ttccgtataa ccgtgctctg tatgggtatt gaggaaaaag ttgataattg ctctttcaag
     3781 ttttgcaccg aggcctttat agaagtgaaa acgtgctcct gtaacttttg cggctgtttc
     3841 gggatcaaga ataccgagat cagcgccgat atcccagtgt gccttgtatt caaaatcgaa
     3901 ctttctcggc tcgccccaac gacgaatttc aacgttttcg ctgtcgtcct tgccaatcgg
     3961 aacgctcttg ttaggaatat tcggaaattc atagataagc gatttctgct tcgcatcaag
     4021 ttcggcaatc ttagcgtcgt ccgcttttat ctttgatttt atttcgttca ttctcgccat
     4081 aatttcagag atatcgccgc catccttctt aatctgaggg attttcttgc ttgcggcgtt
     4141 ctgctcttgc ttgagagcgt cggtcgcctt cataagctca cgtcgttttg catcaatatc
     4201 gagaatatca tcaacaacag cgtccatatc cttatttctt gttttcattg ccgctttgac
     4261 catttcaggg ttttcgcgaa taagtttgat atctaacatt tatatcactc caatttatta
     4321 tttaccgtaa ttttcgagtt tcattgcgag accttcaaga tcgtccttgt caaaaaattc
     4381 tatttcaagt acgcctgcgt cctgctttgc ctcttttatt ttaacctttc tgccgaggtt
     4441 ttcaaccaaa gcaagctcaa cctcgctgaa aaatttatct ctgatctgtt tttttgaagc
     4501 cttcggaacc ttttttgatt ttttaacaag agtctcaacg tcacgaaccg aaagatcttc
     4561 tttaataatc atctttgcag tttgaagaat catatcgtca ctgtcaaatc ctagcaaagc
     4621 ccttgcatgg ccgggtgaga taagattatt ctttaccatt tcttgcactt cattcggaag
     4681 cttgagaagt cgcagcgcgt ttgcaactgc cggacgggat ttaccgacct tttctgccgc
     4741 ctgctcctgc gtaattttaa gctcgtccat catataccga taaccgagag cttcttcaag
     4801 cgggttgagg tcctcacgct ggaggttttc aatcatcgcg atttctatta cttcctcatc
     4861 ggtaagctct ttaattacaa cggggacctc tgtaagtcct gcgattcttg ccgctctcca
     4921 acgtctttcg cctgcaacaa gctgataact tccgttcgca agcggacgaa caacaagcgg
     4981 ctgaagaacc ccgtgctgtt ctatgctttc ggcaagttct gaaagagctt tttcatcaaa
     5041 atccttacga ggctgattgt gattgggctc gatttccata attctgagct tattgacgga
     5101 ggaatttatt tcttcaacgg agttgtcggc aaaaagggag tcaagtcccc tacccaatcc
     5161 gcttaatttt tttgccatat tcaggatacc tccttaccgt tttgtttaag aaattcatct
     5221 gcaagcgcat catatgccat tgcgcccttt gaattcttgt cataataata tatcggcagt
     5281 ccgtagctcg gagcttcgga aagcttaacg tttctcggaa ttgccgtggc atatactttc
     5341 ttcggaaaaa atcgctttaa ttcccctact acctgttgag tcagattaag ccgaccgtca
     5401 tacattgtta aaagtacgcc ttccatatca atatgcggat tgtaaagtct cttgacctga
     5461 cgaatggtgg acataagctg tgaaagaccc tcaagcgcat aatattcgca ttgaatcgga
     5521 actaaaaccg tatctgcagc acacaaagca ttaagcgtta ttagtccgag cgacggcgga
     5581 cagtcaagaa aaataaaatc aaaatccgct ttaatcggtg cgacagcatt ttttattttg
     5641 ctttcacggt gctgcatatc aacaagctca agctctgcgc ctgcaaggtt catatttgat
     5701 ggcaagaccc agagtttttc aaactttgtt tttatcattg ctttctcaat cgccaacgaa
     5761 ttaactataa catcatacga tgaggtttca agctctcttt tgttgattcc gagaccgctt
     5821 gaagcgttac cttgaggatc gatatccatc aaaagaactt tttgaccctt atatgcaacg
     5881 gaagccgcaa gattgacggt ggaagttgtt tttccgactc cgcccttttg gtttacaacc
     5941 gctattattt ttgccatata ttcacttcct tgtattctcc ataaatcaaa ttatatcaca
     6001 actatatata aaataaaaga ttaaattaaa attttcttat gttttatctt ttttgtttca
     6061 cgtgaaacaa aaaaagacgg gtctccccgt ctcatttgca gttcacgttt tcaggtttat
     6121 tgatttttac aacatattct atatattggt ctgtctcgtt cttcgttgcc gaagcattta
     6181 ttccgctttt tctcattgta ctgactgcgt gattaagcgt gttaataaag atacgaacat
     6241 ctttgaaaac cattaccctc ttgccctttc gttctttttc ttcgggctct ttgtttagca
     6301 tttcggtaat caactgctct gaagcgttga cggtcagtcc ctgttcgact attgtatcaa
     6361 gggcttgctc catcattgct accgtcggca gtctcaataa agcgcgagca tgacgctctg
     6421 taagcccgta aagcataacg ctgtaacgaa tttcatcagg caagcgtaga attctcagct
     6481 tgttcgacaa tgccgattgt gattttccaa gtttccttgc aatttcttct tggctgaacc
     6541 cgaagccttt tataagacgt tctattgcca ttgcttcctc aaaaaaatgt aaattttctc
     6601 tttgcaggtt ttcaatcact gcgagtatcg ccgatttttc ctcatcaaca tctacaataa
     6661 ttgacgggat tgcggataga ccggccattt tgcaggctct gagcctgcgt tcacccgaaa
     6721 taagctcata tttgccgttt tccttttttc tgacggtgat cggctgtaac aatccattgt
     6781 aatgaatact ttcggcaagg ccttcaagat catcccaatt aaagctcttt ctcggctgat
     6841 gtggatttgg ttgtataaat cttgccggca acataattac ttcattgctt ttcttaaatt
     6901 tagtttggct tttaagcata gaaaaacctc cttatgcgta ccacaaaaat actgtaacac
     6961 gacaaaagaa ggttgtccac tgtttttcat tgactaaaaa ggctgtattt cacgctgttt
     7021 ttcgtttatg agagcggttt ttttgatatt tgtgccgaag ctcgtggata ttttnnnnnn
     7081 nnnntaaaat aatgtatctt tcgccgcaat tatcaatatt cgcctctttg atttcttcaa
     7141 tccgaccgcc caaaagcttt attgctcccc ttgcagctaa aacttcttca tcggttttta
     7201 ctgatttcat cggagaaaaa tatcctccga gctttacaaa cggtaaacag tattcactga
     7261 gtatattaag acttgcaacc gctcttgcag ttacaatatc atattgttca cgaaaagttt
     7321 tatctttccc tgcaagttca gctcgcatat ggacaatatc ggcttcaaga ccgagttcat
     7381 caagaacgtt tgcaatgaaa acaagccgtt tatttgttcc gtcaagcaaa gtcattttaa
     7441 gatcaggacg tgcaatcaac aatacaacgc ccggaaaacc cgcgcctgtg ccgacatcaa
     7501 tcacttttgc attatcttta aaatttacac aagataaaag tgcaagcgaa tcaacaaaat
     7561 gcttatatac aatttcatcc ggatctgtta ttgcggttag gttgagcgtc ttatttgtct
     7621 caacaagcat ttctgcatat aaatcaaacc tgtcaagcgc agtttcatcg agcaaaacac
     7681 cgattttttc tgcttcacat ttcaaaagtg atttatttat catttctgcg ctccttttca
     7741 agatagataa gtaaaacaga aatatccgac gggctgacgc ctgatattct cgaagcctga
     7801 ccaatgctgt gcggacgaat ttttgagagt ttttctctcg cttcaagtct aagaccgaga
     7861 atcgagttat aatctatttc atcggaaaga gtctgaactt caagacggtg catttcatca
     7921 acctgagcct gctgacgttt gatatagccc tcatattttg tatcaatttc gacctgctca
     7981 aaaatttcat acggtaaatc gggtctgtca acgtcgatcg aagttaaaca gtcatatcct
     8041 atctgcggcc ttttgagcaa atcgcaaaga cgaacgcctg tggttgcttt tgatgtttca
     8101 cgtgaaacaa gtatattgtt taatgcatcc gacggtgaga aaactgtttt ttcggtacgc
     8161 tttttttcat cggcaattag cttaagtttc ttcaaaaatt ttgcataccg ttcgtcgctg
     8221 ataagaccta tttcgtgtcc gattggtgtc aaacggcgat cggcgttatc ttgtcgaagc
     8281 aaaagacggt attcggagcg tgaagtcatc attctgtagg gatcggaaca accttttgtt
     8341 acaagatcat caatcaaagt gccgatatat gaacctgaac gctcgagaat aagagggttt
     8401 tgacccttaa cctttctggc ggcgttaatt cctgcaacaa gaccctgtgc agcagcttcc
     8461 tcatatccgg aggaaccgtt aaactgaccc gcaccgaaaa gaccgtcgta gtccaaaaat
     8521 tcaagacttg ccttaagcgc aagcggatcc acgcagtcat attctattgc ataagctgtt
     8581 ctcataagct cagcatgttc aagtccgggg atagttctta taaatttaag ctgaacatct
     8641 tcgggaagag aagaagaaag accttgcaag tacatttctt ccgtgttctc tccgcagggt
     8701 tcaataaaga tctggtgacg ttccttttcg gcaaagcgaa cgattttatc ctcgatgctc
     8761 ggacagtagc gaggaccttt tccgtcaatt ttgccgctgt aaagagggga gcgatgtaaa
     8821 ttttttaaaa ttatatcttt tgtttcaaga tttgtatagg taatatagca aggaagcttg
     8881 ttttgtatct ttattttgnn nnnnnnnncc aaaatcacac aatccgcggt gtaaacggcc
     8941 tcaaggtgcg ttttcagttg ccatttaccg ttatcaagct tttgaatatc aacaatttcg
     9001 ccttgacgaa ggtcaaggtt gtcctgcttt tcaagcgcat gcttcatata gtcggaataa
     9061 agacgacggt caatttgagc acgcagactg tgaacggcag gtcctttacc aagattgaga
     9121 attcggcttt gcagagtgtt tgcgtcagcc gccttgccca tttctccgcc gagtgcgtca
     9181 atttcacgga caagatgccc tttagaggtt cctccgattg acggattgca cgccatgttg
     9241 c
//
LOCUS       DAWBSW010000068         9239 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1549_length_9239_cov_2.417574, whole genome shotgun sequence.
ACCESSION   DAWBSW010000068 DAWBSW010000000
VERSION     DAWBSW010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1549_length_9239_cov_2.417574"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6027..6036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6951..6990
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8340..8349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2388 a         2256 c         1883 g         2652 t
ORIGIN      
        1 cctatcataa gaccgtcaac gtcgctcgac agaacctctc gcattttgtc tcttattttt
       61 tttgaattat cctgaaacga gatatccttt ccggccgcaa gcttgatttc gccgtcgccg
      121 aaacgggcaa ttgacctttt attatttaca atttcatcaa ttgtgtcatc aaccgaagca
      181 acatgcggaa cttttgtaaa ttttcggtaa aaaaagtcct ttatacgcca aaacgtatct
      241 ttgattttca gccacgtctt aacaagaata tctattattt ttacgataag ctgtttcaag
      301 ccgttcactc tccgatagct tacttttctt cctcgtcacg gtattcgtga tttgttgcgt
      361 cggtatcaat cattccgtta tcctccggaa tgatgattgc aagcatgatg taaactataa
      421 ctcccgggaa gccgcccgtg aagaacgaca caagagcata aataactctt acgatcgtcg
      481 ggtcgattcc gataaaatcg gcaaatcctg cacatacgcc gctgagcatt ttgttgtttg
      541 tacttctgta aagcttcttg tttttcatat ctatcatcct ttctgaattt tagccgcagt
      601 tattgtgttt tgcatgagcg tcgcaatcgt catagggccg actccgcccg gaacaggcgt
      661 aatatagctc gccttttctt tgcacgcttc aaagtcaaca tcgccgcaga gcttgccgtc
      721 ctcgccgcgg tttattccga catcaattac aacagcgccg tccttaatca tatccgcagt
      781 aacaaatccc gctctgccga ctgcggcaac aacaacgtcc gcctgtgaaa cgagttcttt
      841 aagatttttc gtgtgcgaat ggcagaccgt tacggttgca ttctctttga gcatgagcat
      901 cgccataggc ttgccgacaa tgttgctcct gccgattatg acgcagtgct tgccgtcaag
      961 gcttatattt tcatacttta acatttccat aattcctgcc ggtgtgcacg gaaggaaact
     1021 gtagtcacct atcatgatat gccctacgtt ttcgggatga aaggcgtcaa catccttttc
     1081 gggcttgatt agattgagta cctctttttc attgaggtgc tttggcagcg gaagctggca
     1141 gagtatgccg ttcacgctgt tgtcattatt gagcttttca acgagcgaat taagctcctc
     1201 ctgcgttgca tcggtgggaa gaagatattc aagcgaacgg attccgcaga attcgcaagc
     1261 ttttttcttg ttgtttacat atactctcga agccggatcg tcgccgacga gtataactgc
     1321 gaggcagggc tcaatgccgt cctttttcat ttcactcacc tgtgcggcaa ctctttcttt
     1381 gaccgcaagt gaaatttctt taccgctgat tatcttgtac atcactgacc tccgcttctt
     1441 tctcttcctc tttgctttcc ttttcctccg cctgcttttt cagctcgtcc tcaaggaaat
     1501 aatctacgcc gtcaatcggc tttggatgtt tcttgtactt gacaagcaga cagataagaa
     1561 taacaaggca tacaacggcg aggacagccg aaagcacctg tgaaactctg accgttgtac
     1621 ctgtgatata aaggctgtcc gttctgaaac cttcaaccac cgctctttcc gcaccgtacc
     1681 atacgccgta ggtaaggaaa atctgtccgc tgaattttct tgcctttctc gtgatgatat
     1741 agagcacgac aaatccgagc aagcaccaaa ccgattcata aaggaatgtc gggtgaacag
     1801 ccctttccgg atccattgtg attccgtttg cgctgagctt gtatgaattc tccgttatgt
     1861 aacgggcaat tttttcgctc cacatgcccc atggcatatc cgtgtttgtg ccgaatgcct
     1921 cttggttggc gaaattgccc caacggccta ttccctgacc tatcaaaagg ctcatggacg
     1981 caatatccat aagattccag aaattcatct tgcgcactcg gcaaacaatg aacgctgcga
     2041 ttataccgcc gattataccg ccgtaaatcg caagtccgcc gttccaaatg taaaggatct
     2101 caataggatt atccttgtac ctgtcccatt caaagatgca ataataaagc cttgcaccta
     2161 gaattccgaa aattgtcgaa taaataagca catcgagcat cttatcaatg ctcattttcc
     2221 atttgtaagc cattctgccg ccgaaaagca ctgcaagcaa aaatccgaag gcaattatcg
     2281 ctccgtacca gcggattgtg atatcgtggc cgaaaaggga gaattgagca aggattgagg
     2341 agacctcaaa tccatgctct atgccgttaa aatacactgt tgtagtgtcc atagtatcaa
     2401 ctccgatcag ttgggattta ttacggaaag aacgctgcgg ttttcgtcaa gcgtcttttt
     2461 aaagatatca ttaacgtcat caagcgtaac ggttttaaaa atttcaaaat cggtgaaaac
     2521 atcttcgccg gcaaagtatg aaacagccat gttattcgca agaccctcaa tgtcgttcat
     2581 gcccattatc attctgccgt aaagcttctt ctttgttctt tcaaaggccg ttttgtcaaa
     2641 gccggtctct ctgaattttc cgatttctcc gactatctcc tcggcaacct ttttcggatc
     2701 attactttca cctgcgaaaa gcacgcacga atagccgaag ccggtaaaat attcaaagcc
     2761 gaagctgtta tttataagct tgccgtcaaa aagtctctta taaagctccg aggactgacc
     2821 ggaaatcata tcgaggagaa tttccataga aatttcctcc ttagtcgtgc gctcgggagt
     2881 atcccagctc tccttgaagc cgaacatgaa ctggggcgtt gcaaccgaga gcttttcttc
     2941 aatatagctg tcaatgacct cttccggttc gtcgatgacc ttacgctgag ccattttgcc
     3001 ctcaacaggc ttgagcacct tgtcggcaac ctcgactatc tcatcaacat cggcattgcc
     3061 tgcaacggca agaaccatgt tatgaagatt gtagaagttg tcatagcagc cgtaaagcgt
     3121 tttcgccgtg atctgagcaa tcgattcctg cgtgcccgca atatcaattc ttacgggcag
     3181 attgtgatac atcgttctaa gacagttgaa catgacctcc cagtcgggag cgtccttgta
     3241 catgtcgatc tcctgaccga taattccctg ctctttttca accgtagcct gtgtgaaata
     3301 cggattctga acaaaatcga gaagaatttc aagattcttt ttgaaattcg ccgagcagga
     3361 aaaaagatat cccgttcttt caaagcttgt atatgcgttc gccgaagcgc ccgttttggc
     3421 aaagcgctca aatgcgtcaa gatcctcgct ttcaaaaagc ttgtgttcaa ggaaatgagc
     3481 cgttccctcg ggaatttcct taaatgatcc gtcgctcatc tgaatcatcg tgtcaatcga
     3541 accgtacttc gttgcaaaaa tcgcatacgt gcttgaataa ttctccttgg gcatgacaag
     3601 aattttcaag cccgacggat ggtctatatc gtagtatttt tcattgagga gctcgttcct
     3661 gacttcttta atattcatca ttctgccgcc tccccgcttg atttaagcat aaatattgtg
     3721 tcaagcatta ttgtctttgc cgctccgacg acctcagacc tgttaacatt tctgatctcc
     3781 tcggcataag cctcgggagt tttcagctcc tcgcggagta tctgtgatgc gtaccacgcc
     3841 gttatctcct ccggtgtatc ggaattgccg agaatagtat ccgtaaggct cttgattgaa
     3901 gaagagtaat cctcgtcgga aaattcgccc tctgcggtca gtctgagctg attgattatc
     3961 tcattctttg ccttttcttc gtttgcgtcc tcaattccgc tttgaaccat tacgattccc
     4021 tttgaattaa agagcgccgc cgagcaataa tagcaaaggc tcatcttttc ccgaaccact
     4081 gagaaaagct ttgaataggt tccgccgccg aagatatcaa cggcaaccct catagccggc
     4141 atcatgtcgt cctcgcttct cattccggtt ctgaagccca taacaagctt gccctgcttg
     4201 agaggcattg tctcgcaaac gtattcgggc ttcggaagtc ccgaaacaaa ttgagttttt
     4261 atctcaacgg gatttctttc gatttcagaa aatttatcct taataagttc agccacgggc
     4321 tgaggatcca tgctcgaaac catagttatc tgaacggttg ctttttcgag cacgtcacgc
     4381 catgcggaat aaacatcagc tggtgtgagt gctttaacct cgtctaccga gccgagacgg
     4441 ttgattccgt aggcttcatc ggcaaacatg agctgttcgc atctgagcat ggcatagcgg
     4501 cgcttgtcat tctgctcgtt ctcgattgtc tcaacaagca gacgcttctc ctgctcgatt
     4561 atatcctccg gaaagctttt gccctcggtt agaggatcaa aaatcatttc ggcaagaagc
     4621 tgtgcacact caaaagcaac cttatcgccg tcgagagcaa agcggtcgtc aatggcagtc
     4681 atactgaagg aaagaatttg agcctctccc cttttgagaa tgcctgccga aacagaagcc
     4741 ccgtaaagcc cgtcaagcac acggttgaga gaagtaaaat caggatattt tgcacatctg
     4801 cgtgaaaaca tgaacggcaa aatcgccctt gccgaagcgc tcttatcaag cggcatcgcc
     4861 aagctgatgt tcacacggca ggtcttgaat ttatcggttt tgaccgagca aaagcggaca
     4921 ccgtccgcaa tattttgcgt tgtcataagt tcactaccct tttcaatata aaaattcagt
     4981 atttagtata acacattatt gacgaaaata aaagtatttt tattataatt aaattgccgg
     5041 attttttcga ttatttttta ttgtcttttc agacacgatg cacaccgtaa aaaacgattc
     5101 gattttatgt tccttacata aaattcatcg cagtgcatga tgcccgcatt aactcaaagc
     5161 tattataaaa gggtgcccta aaccggacac ccgaaattat tttatgatat ttaccgttga
     5221 attcggatcg aaaagatgaa gtctgtcggt aagtattccg agctcgacgg tttcgtttgt
     5281 tgttgagtct gcatctactc ttgccgtcag cttttgaccc gaataatcaa gatagagata
     5341 gctttcgctt cccataagct cgctgacctc cagctttgcc gtcaacactt tatccggatt
     5401 atttttgata aatttgctgt caaccgtgac atcctcggga cggattccta acttgacggg
     5461 tttatcgaga taccccgtta tctcctttcg gctttcgaat gggtctgtaa tgtcaagctc
     5521 gttgtctgcg agcgaaagaa caagctttcc gtcacgcttt aatacggttg cgtcaaggaa
     5581 attcatctgc ggcgatccga taaagcctgc gacaaacata ttctgcggat gcttatatag
     5641 attttgcggc gaatcgacct gctgaattac tccgtccttc attactacga ttctatcgcc
     5701 cattgtcatt gcctcggtct ggtcatgggt aacataaatg aacgtagtgc ccaagcgccg
     5761 gtgaagcttt tttatctctg ttctcatatt cgttcgcagc tttgcgtcaa ggttagagag
     5821 cggctcatcg agcagaaata cagacggaga gcgcaccatt gctctgccga gcgcaacgcg
     5881 ctgacgctgg cctcccgaaa gtgctctcgg cttccttttt aaaagatgct caatgtcgag
     5941 aacctttgcc gcctcacgaa ctcggcgatc aatttcgtcc ttaggaattt ttctcaattc
     6001 aaggccgaat gccatgtttt tataaannnn nnnnnnccgt catgtgtgga taaagagcgt
     6061 agctttggaa taccattgct atatccctgt ccttgggcgc aacatcattc actacacggt
     6121 cgccgatttt cagttcaccc tttgttatgt cctccagtcc ggctatcatt ctcagcgttg
     6181 ttgacttgcc gcagcctgac ggacctacaa gaataatgaa ctccttatcg gcgatttcaa
     6241 gggaaaaatc attgactgcc gttgtgccgt catcgaatat tttatataca tttttcaaac
     6301 tgatacttga cataatttat cctttcacgg ctcctgcaac gatgcccttg ataatatatt
     6361 tctgacacaa aagatagaaa acgacgatag gtataatcga aagcacaagc attgccatca
     6421 ttgcacccat atcaattgaa ccgtagccgc ctctgagata ctgaacggcg atgggaatgg
     6481 ttttgtattc gctgccgatg agaagatacg ggagcagata gtcgttccag atccacattg
     6541 cgtcaagaat tgcgactgtt atcgccgtcg gcttcattat cggaaacacg acgtaaaaaa
     6601 acgtttgcag cggactgcac ccgtctatca ttgccgcttc ttcaatctca agcggaacgg
     6661 atttcacaaa gccgcagaac atgaaaaccg ccagtcccgc accgaagccg aggtagacga
     6721 ttatgagtcc ccacggctta tcaagcccga ggtcggcaac tgttttcgtc atggtgaaca
     6781 ttaccatctg gaacggaact atcattgaaa aaacaaagat ataataaagc gcctttgtaa
     6841 atttattctt tactctgaca atgtaccacg ccgtcatgga cgtaaaaaga acaattgccg
     6901 caacagagaa caccgttatg aaaagcgacc agccgaaagc ggcgggaaaa nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttcaatcc ccgaaaaggt ctcggcattt
     7021 ggaagcgcaa acgggtcgtc gctgataaaa aacttgcctt tgaacgaatt ataaagaacg
     7081 attattatcg gtgcaaggaa cgccgcactt gttgcgataa ggaatatata cagcagaact
     7141 ctgcctgctt tttttgccgt tatcccgttc attgctccac ctctttgttc cttgtgtaaa
     7201 taagctgtgc aaacgaaatt gccgcaacga tgatgaaaaa tataaccgcc ttagcctgac
     7261 cgatgccttc ccgtccgatt tttccgtaga aggtgttgta gatatccaag gcgagcatcg
     7321 ccgtttgctt tgagggcgcc cccgccgtga gtgctaaatt ctgatcgaac agcttgaagg
     7381 aattcgtaat cgtcagaaag aggcaaatcg taattgacgg catgacaagc ggcaaggtaa
     7441 catgtctgag cgtctgaatt ctgttggcac cgtcaacctt tgatgcgtcc tgcaaatccg
     7501 agggaatatt ttgaattccg gcgatataaa ttatcatcat gtaaccgata agctgccagt
     7561 tcatcataaa aacgagtccc caaaagccgt attttgcatc ataagttatt gttaccccga
     7621 aattttgaag aactccgttt attatcatct gccatatata tccgagcacg attccgccga
     7681 taagattcgg cataaaaaag accgttctga aaatgttcgt acccttgaag ccctttgtca
     7741 aaagcgtcgc aagcccgaat gaaattagat ttatcgtgat gaccgacacg gccgtgaact
     7801 tgacggtaaa ccatagagca ttgagaaaat ccctatttga aaaagccttg acgtagttcg
     7861 caaaagcaac ccattttgca tcaagtacgg tcgtgaattc cgtaaacgaa aggccgagac
     7921 cgaggataaa cggcacggca aaaacaattg tgaatgccgc aagagtcggt aaaacgaata
     7981 ccgggaacca ttttttcagt gccatattat cctcccctca tttatgattt tgccgatttc
     8041 cagccgtcga ccgtgacttt tttaacatca tcgaagcttt tggtgccctg cacatattca
     8101 agcaacgcat ttccgaaatc gtctttaaac tcctggctcg ggaaagagtt gaaaacccat
     8161 tccaccgttg ttttgtcttt ttccatccac ttgataacct ctcttgcaag cggatcgtcg
     8221 ggtctttcgt tttccttaaa ggtgttgaac ggagaaataa aatcaagttt gtttaccgca
     8281 taatccttgc cggtttcact tgaaaacagc cactcaagaa attcaacgga tgccttttgn
     8341 nnnnnnnnna tacaaagccc ctgtttttct tcgccgtcga ttccgatata aatcggaaga
     8401 tacttaatat cgtccgcctt gaccgtattt cctttgacct ttgaaatctg ctcccacgcc
     8461 cagttgccgt tctgaaccat tgccgccttg ccgagcgcaa attcggacat tgaatcttca
     8521 accgatttgc ccgaaagaag tcccttctct gttgtggaat tgttgatata aaggtcaaaa
     8581 acgttcctga aattatccgc atatttaaaa tcaatcgttt ttgcattata agctgtcgat
     8641 attgccgatt cgtcacttat gtctttcatt tcataataaa tcggcatatt caaaagatga
     8701 gtagtccagc gccattgctc tccgcccgac atcgaggttg aagcaaaaac tccgtcaatg
     8761 ccaagctcct gcttatgctt tgtcatgtcc tcaacaacct ctttgagaag cttaaagctt
     8821 gtgatttcct ccgcctttga aatgctcacg gctttatctt taagagcaaa gtatttttcc
     8881 atgatagcgt tgttgtagat gattccgtat ccctcgacaa cataagggat tccgaagact
     8941 ccgccgctgt ttttgatggc aaggtcttta tccgagagat agtcatatag ctttgtgtca
     9001 ctcaaatcct tacagtattt tgcccaggac taatatccga ccgggccgtt tatctgaaaa
     9061 atcgtcggag cgtccgactt tgcgatctcc gatttaagcg ttgtctcata ggtgttgctg
     9121 gcggcggtga cgaccttgac cttgacgccc ttttcagcct catatttttg agctatttcc
     9181 ttgtagatat ccgcaatttc cggcttgaaa ttgagaaaat aaaccgtgtt gtccgcctt
//
LOCUS       DAWBSW010000069         9220 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1552_length_9220_cov_2.654774, whole genome shotgun sequence.
ACCESSION   DAWBSW010000069 DAWBSW010000000
VERSION     DAWBSW010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_9220_cov_2.654774"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    645..654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1341..1350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7384..7393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2591 a         2081 c         1937 g         2581 t
ORIGIN      
        1 ttaagctttc cgaaagggag aaactttctg atatatgaaa agccccaaac gaacggcata
       61 gcaaacggtc tttgctttat catcggtgca ttcatatcga attttttggt ttcgggcgca
      121 ccccctcgga catactttcc gaattctttg ctcaaaacgt cacttccctc gctttaattt
      181 tgtgttttaa acactttaac gattataaca tgctcgtaca atttatgcaa tattaaaaat
      241 taccatttaa cagccgtatt ttcgccatat tttaaacaaa ttccacggat attcgccgat
      301 attcggtatg cttttgaacg aaacggattt accgtcaaat tcaaactcaa ccttatgcga
      361 ccatagagcg acctcacagc cgttatcgcg gcttccgtac tttccgtcgc ccgcgagcgg
      421 catttttctc gaagcgaact gaactcttat ctgatggctt ctgcccgtgt tcagcataat
      481 gcagaccagc gctttgccgt ccgcctcgtc aaggaccctg taatcaaggc ttgcacggcg
      541 tactcccttg cgctcacgct tgacaacata gcttttattc ttttgtttat ccttaaagag
      601 caaatcttca agcctgccct ccttctcctc ggggactccg tcggnnnnnn nnnnttcatc
      661 gcccgtctgc tcctttaaca gcttaaccgc atcctccgct ccgtcgcatt gtgacggcac
      721 gccgaccggt ttaatgcagg ctaaaaaatc ctcgttttca aataaaatct caatcattgt
      781 caatattctt ttccttgaat ttttcagccg tatttttaat tttgagcacc tgcttcatga
      841 atcccataag ctcaatcggg tttgacgatt tctgaagctc gggcgtaaca agaccaataa
      901 tatcctgcag ctcggaaagc tcgtttataa gctccttttt ttcctcatcg ctcatttttt
      961 taacatccgc cctgagcttt ttttcaatac ctgcaagctc gtcggaaaca tcgctgacct
     1021 tctcggcagc aatcggtctg ccctcttcat cacgaataat catatttttc acctatcaaa
     1081 acaaataggg attgtcgcgt atgtgtttgc ggcatcccta tttttcttta aatatttcgg
     1141 tagcgggtta tagatttcca aaaaaccata aagcctcggt taatttcttc tgcatggcaa
     1201 attcatttaa ccgtaccgtt aacttcggat atttattttg cttctttggt tgtaacttcc
     1261 tggatatact tgaagttaag agcaccctga tccttcatga ttttctcaac agccttgtca
     1321 agagcagcct tattgtaaac nnnnnnnnnn ttgtgtaata atacgaaaca taagcgccct
     1381 caaagctctt gagcttctcg cgcatcatct tcataacgtc gtccgtaact ccgatgtact
     1441 cggcagcctc ggcctcggtc atgtatgtcg tgtctgcggc aacggcagag gtctccttga
     1501 cgtttgacgg tgactttaca gcgtatgtaa cgcagattgc ggctgtgcaa agaacagcga
     1561 ctattgaagc aatgacagta tttttcatta aaaaaactcc ttcttttaat ttctctatgt
     1621 ataataacac attagtaatt ttattgcaag attttcggga aaatttattc ccattccttc
     1681 catatttcag gcttatagcc cacagtcgct tttttgccgt ttctgactat cggcgttttg
     1741 aaaagcgtct gattttccaa gagcttctcc tcgaccgctt ccttggaggc aagatatttg
     1801 ataaagcact tttcataagc ctttgatttt tcgtctatca agccgtcaat tgagcctatt
     1861 gccgctttga tgtttctgta ttcccctaaa ctcatgccgt atttttcaag atcaatatat
     1921 tgatatttaa tcctgcgctc tttgaaatag cgctcagctt ttttggtgtc aaagcatttt
     1981 gacttaccgt aaatttgaat attcattgcg tcacctcgat ttgatattga aaattataac
     2041 gtaaaatggt agaattgtaa agaggtgaaa acgatgcgta taaattgtcc cgaaaaaatt
     2101 ctgaaagcta ttgccctgct tgaaagcaac ggctattccg cctatgcagt cggcggctgc
     2161 gtccgtgact ctgtaatggg cagagctccg aacgactggg acatgacgac ctcggcagca
     2221 cccgatgaga caagggaagt tttcaaagac ttccgtaccg ttgcgacggg aataaagcac
     2281 ggcaccgtta cggttttaat cgactccgag cctgtcgaaa taacaacaat gagagttgac
     2341 gggaaatatt ccgacaaccg ccatgacctt tcacgccgcg attttaccgt caacgcaatg
     2401 gcatacaacc ccaaaacagg cgttgttgac ccgttcggcg gtcagaacga tattaagaat
     2461 aaaatcattc gctgtgtcgg caatccggat acacgtttcg gcgaggatgc gctgaggatt
     2521 ctccgtgcaa tcagattttc ctcggttctt ggctttgata ttgataaaag aacttcgcaa
     2581 agtataatcg gcaaccgttc tcttcttcaa aacatttcaa aagaacgcat cagagttgag
     2641 cttatcaaaa tgctatgcgg tcaaaatgtc gaaaaaattc ttgccgatta caagagcgta
     2701 atctttgaaa ttatccccga gcttgccacc gaggacggtt ttttgcagca cacgccctat
     2761 catatcttcg atgtctggac tcacaccgcc aaggtcgtat caagcattga acccgtctcc
     2821 gatttaagag tagccgccct gcttcacgat attgaaaagc cgtcgatgtt ccggacagac
     2881 tcgcataaca tcggccattt caaaggacac cctcaaaagg gagcggaaac ggcggacatt
     2941 attcttcgca gacttcgctt ttcaaacgcc gagataaagc atataacaac aattatcctt
     3001 ctgcacgatg agcgacccga cggcaaccgt tttcatctgg ctaagctttg ctcgcaatac
     3061 ggcattgaca atgtcgaaga tacattaaag ctgatacttg ccgacgcaca cggaaagaat
     3121 cctgcgtttt ttatcaaaga ggtcgatgcg gttcttgttg cacagaagca gattgaattt
     3181 atgagaaaaa accgtgtttg tcttaagacc tcggagctta acatcaacgg caacgacatc
     3241 atggcgctcg gaatcggcag agcacgaata aaagccaccc ttgacttcct gcttgatgag
     3301 gttataaacg agcgaatcga gaacgaaatc tccgctctct cacgagcagc ggcggagttt
     3361 aacgaaaaaa caccgatttt tgatactttc ggttaactaa tttatatatc catataaaat
     3421 tcgattattt tccaaaaaaa tcatgtaagt tattgaaaag caaaaatata aatgatataa
     3481 taaattggtt ttagtctatc agacgaaaaa taaccggcaa aatggaaaat aaggagctga
     3541 caaaatggca gaaaatgaga aaaagaccat accttccgtt gaaggcatgg aagctcatat
     3601 cgacacatgg cgagacatta ttaaaaacaa gcagagaggt attttcacct ttgacgagct
     3661 tgttgaaatt aagcttaagg cactcaaaaa gagaatatac acggactttg tgaacaccgc
     3721 tccctggaag atgcgtgagt gcatctacaa ggacatcggc gactacgagt atctttacga
     3781 cggttggaag gatctcgacg taggcggcta ctggggcggc aacggtctga gctcgttctt
     3841 caagaataaa atcactatcc ccgagagatt cgagggtaaa aaggttactc tctacgttta
     3901 cttcggcgga gactctctcg tttccgtaaa cggcgtgccc ttccagggtc ttgacccgtt
     3961 ccgtaacgca gttgttctta ccgattgtgc taaggccggc gatacatacg atatcgacat
     4021 cgaatcctat ttcgtatggc actcaaacga aagctctctc aagaagcttg agtgctcctt
     4081 catcgctaca acagacgatg aaatcaacga aatttactgg gatttgaaag cagtttacaa
     4141 tgcactcttt atgccgggca tgggcaacga ctatgctacc cttatccgtg aatcgcttaa
     4201 agaggctttc cagtacgttg actttgacga gcctgatttt gacgtattca aggctaagct
     4261 gagaatgtgc aaaaagattc tctacgacaa agtttacaac aacccgaact ataaatgcat
     4321 gggcaagctc gaccttatcg gcaactccca ccttgacatg gtttacatgt gggcttacaa
     4381 ggagttcgtc cgtaaagtcg gccgtactca cgcaacaatg cttcgtctta tggagcagta
     4441 tcccgacttc atcttctctc agagccaggc aggtatgtat gaggaaatga gagttcatta
     4501 tccgaacatc ttcgagcagg ttcaaaagag agtcaaagag ggtcgctggg aatatatcgg
     4561 cggcatgtgg gttgagccgg attgtaacat tatctccggc gaaagctttg tccgtcagtt
     4621 cctccacggc gttcgctatg ccgagaagtg gttcggcgta acgcccaaga cctgctggct
     4681 tcccgacgtt ttcggcaaca gctattgtat gccgcagatc atgaaaaagg caggaatgaa
     4741 atatttcgtt acccacaaga tgggtatctg gaacgataca aacccgtggc tctataactc
     4801 cttctggtgg gagggcccgg acggatcgaa ggtattcgca gccgttcccc cgactcactt
     4861 catcggttca atggaggcgg attcactcaa gacaaactgg gtcaagtata ccgataagga
     4921 caccatcggc gagtccatgt actgctacgg ctggggcgac ggcggcggcg gagtcgataa
     4981 cgagatgctt gaatatgtca agagatataa gaacttcccc ggtctcccgg aaacaaaggc
     5041 ttgcaagatt gaggaatccc ttgaaaatat gcgtcagaag gctattgctg ccggcgacaa
     5101 gatcgttaca tggaaggacg agctttatct tgaggagcac cgcggagttc acaccacaaa
     5161 ggctgctctc aagaaggcca accgttactg tgagaacctc ttcagagagg ccgagatgta
     5221 cgcagttatc gcagaaagct acggctatga atacccgact gaggagctcg ctctcgcatg
     5281 gagaaagatt ctcaccaatc agtttcatga ttcacttccc ggaactcaca tcacagaggt
     5341 attcggcaag ctcatgagta tctatgatga ggtaatcgag accggcgaaa gagtccgcaa
     5401 cgaagctctt gcagttatcg ccgagcatat cggctatgac aagaacctcg gcaagccgtt
     5461 tgcactcttc aactcacttg caaatgtcgc tacaaccaag gttgagcttg attatcagga
     5521 cgttgatatc ttcgacgaaa acggcaataa ggttgcaact caggcctaca caaagccgaa
     5581 cggagacaag aagcttgtat tcgttgccaa ggacattccg gcagtcggct acaaggttta
     5641 ctataaaatg cctgccgcaa aggctcccgc atatgatgag agcaacggca aagagatcga
     5701 gaacgacaat ttcaagcttg ttcttgacga caatgcaact cttatctcaa tctatgataa
     5761 gaagaaccgc cgtgaggtta tctccaaggg cggcaagggc aacgatttca gactctttga
     5821 agatatgccc ggcggatacg acgcatggga tatcgttgca acatatgttg accgtgagtt
     5881 cgagcttaag gacggcattg ttaaggatat agtcaagggc gatgtttaca caatgatctc
     5941 aattgagaag gatgttctta aatcaaaaat cgagcagaac atcatcatct acaatgacct
     6001 tgacagaatt gactttgata cacacattag ctggcacgag gatcagaagc tcctcaaggt
     6061 attcttcgac gtcgatcttc agacgaagaa ctatacgaga gatatagcct acgccacaat
     6121 ggagtgctgc tcatacagat acaatccgta tgacaaggca aagtttgagg tcaacgctca
     6181 caactggatt gacatgtccg acgaggatta cggcgtatcg cttctcaacg actgcaagta
     6241 cggccacgag gtcaacgagc acaagatgat ccttactctt cttaaagctc cgatcaagcc
     6301 cgacccgaaa tcagacagag gcgaccacta cttcacatat tcgctctatc cccatgccga
     6361 gacatggaag aaggcaaaga cccttgaaag gggacttgaa atcaatcatc cgcttgttcc
     6421 cgttgacatc aaggcggagg gcgaactggg cgcagataag tccttcatca ctcttgcaag
     6481 cgacagcatg actcttgagg cagtcaagaa gtgcgaagaa gaggacgcat acatcattcg
     6541 tatggttgag aagaagggca aaagcgttga tgcaagcgtt aaattcttcg ctcccctcac
     6601 ctatgccgca gaatgcgacc tcattgagag aaacgacgtt cccgttgact ttgacggcga
     6661 cacaatcagc ttccacgcaa ccccgtttga gatcaagaac tacaaggtaa agatcaaata
     6721 aaacgatttt aagagggctg ttgcagcaaa tgcagcagcc ctgtttttta tagttatgag
     6781 ctttgaaaat gatttaaaga aacaaattga aacatacata tttttcccgg agaatataat
     6841 cgacgaggtc ataaaagcaa actgcaaaag tgttgagctt ctgacggaaa agcaaatggc
     6901 tgacatctgt gccataagga gaaccgcctg tgtttcggcg aataaattca ggtattcgat
     6961 tgatgatctt gacctgatta tcaagacgat cgacaccaat cccaactcgt cctttgccaa
     7021 gctcagagat gccgtaagac aaatcgaatt tatactcggt gaaaaataat tcggaggaaa
     7081 aaatggaaga gaaaaaaaga ctgatttacc ctgatctgct gagaataatt tcaatcttca
     7141 gtgttcttgt gttgcatgcg gtaggtgaaa tatggggaga tattcctata cagagcaaaa
     7201 gctgggtggc ccttgttgca atagacggca tgtttcgctt tgccgttcct gtctttatca
     7261 tggtctcggg aatgtttatg ttatcccctc aaaaagacag aggaattaag gagttatatt
     7321 caaaaaaagt tctcagaatc attaccgcct ttattttttg gtcgctggtt tatacttttt
     7381 ttcnnnnnnn nnnctttttt tcatttagca tcaggatttg ttaccgaagg agtctcattt
     7441 aagcctgatt ttcaattaac tgttaacaga tttctgctcg gagaatacca tctttggttt
     7501 atgtacatga ttgcggggct ttatatcgtt acgcccctgc ttcgcaagat tgtcgaaaag
     7561 aaatcaacca tgcaatattt tatttttaca tggtttatct tttgcatcgt tgccaatttt
     7621 acgggcctta taccagtaat cggcaacaag atctacggat ttattgacct gtttaagatt
     7681 tctattgctg tccaatacag cggttactat gttctcggct attatttgca taattacacg
     7741 gttaaaaagc cgataaaagc agcagtgtat tttctggcaa ttctcggtac tctcggaatt
     7801 gtcggcatga cagttttcac atctttcaga tcgggtgaaa cagtcagcaa atatctcgga
     7861 tatcttttgc caatgactgc cttccaatct gcggcgttgt ttttattctt ccgggataaa
     7921 tttgaagatt tcaatccttc aaagaaggta aaaaaggcaa tatttatact ctcaaaaatt
     7981 tcattcggca tatatctgtc tcacattctt gtgatgaagg ctgttgagaa gttctgccac
     8041 atgattcttc ccttgccgcc atatgcactg ttcggagcgt tgctgatttc tgcggctgtt
     8101 atcagctcgc tgatttccta tctgctcaat aggattcccg gcgcaagcaa atatattgtt
     8161 taagggggcg gacaagatgg aaaacaaaaa aaacagcgtt tacacggata ttttgataat
     8221 aatttctgta ttggcggttg ttgccgtcag cgtattatta aagaaatggt tcaaggcgga
     8281 ggtcggctct atatcatggt ttttcatgac cgcgctccag agtatattca gatttgcgat
     8341 tccgacaata attatggttt caggtgccaa aacactctca tcgccggaca gcttaagcat
     8401 cagaacggtt ttttataaag atattctccg cttggctgcg gcatttgttt gttggtcgac
     8461 attctgcatc ttactggaca aataccttct taaaagaggc ggactcctct actgcgccga
     8521 aaaatttttg tccgacactc tgattgacat gaattatatg tggctcgttt tatcactaat
     8581 cggactttat attgtatcgc cgctgctgaa aagaattatc gaggacaaat ccttactcga
     8641 atactttctt ttaatatggt ttatattcac aatatgcggc aattttttca gctcgattcc
     8701 cgattcgtat attaatttcg aaaaaataaa caatgccttt aaaatgtcgg cggcaattca
     8761 tttaagcgga tatttttgtc tcgggtatta cctgtccaat tacagcataa aaaagccact
     8821 gagattttta atctacgctc tgagtattct cagtatcatt gcatttttgg caattactta
     8881 ttatatctca gtcaaggaag gaagaaccat cagtgcattt attccgaaaa agctgccaat
     8941 gacggttttc gttcctgctt ccgtctttct tttaataaag aatgttttcg gagaagaaaa
     9001 aagctcaccg aaaatcatat ccttctgggc aaaagcctcg ttcgggattt tcatgtgtca
     9061 ccttgcagtc cttgactgtc ttatgaaatc aggcatcttg ccgcacacac gggtgatata
     9121 cctgattgtt ataacaacgg ctgcgacata tttaatcagc ctcattatct cggcaatact
     9181 gaattttatt ccgtttgtta aaaaatatat agtataaaaa
//
LOCUS       DAWBSW010000070         9182 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1561_length_9182_cov_2.852306, whole genome shotgun sequence.
ACCESSION   DAWBSW010000070 DAWBSW010000000
VERSION     DAWBSW010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_9182_cov_2.852306"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4707..4716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2558 a         1938 c         2054 g         2622 t
ORIGIN      
        1 ccgcaggaag ctatacatat aaaatcacgg caagcgacac cgagagctca aatgtggttc
       61 ttcttaacaa acccttcacc gtcggaacgg ttcagtcaag caagctaaaa ataacaaatg
      121 ccaattatcc ctatgtgctt gacgtaggag agggtttttc ggtaacggga attgtttcgt
      181 ctaactataa tataacgagg attgtgatag gtgcatataa agcgaacggt acaaagggct
      241 ttgaatatat cgcaacccct aatgcaaaaa gcttcgatat aaacagcgtt gactacagcc
      301 tgccgttctc gagacttgcc gcaggaagct atacatataa aatcacggca agcgacaccg
      361 agagctcaaa tgttgttctt cttaacaaat ccttcaccgt cggaagcagc tctgtgccga
      421 caggcaattt aacaaaagtc aactgggatg ttattgatat ttcatattgg aacagtgact
      481 caataagctc gtggtcgaaa attgcatccg acattgatgc gatcatcctc agactcggat
      541 ttgcatatac cgcaagcaag aaaatggaag aagacgacag ctttgcggat tactatgctg
      601 cggcaaaaaa atataacatt cctgtcggag tttattattt cggagcagca acgaccgcca
      661 gggaagcaag cagggaagcg gattttgtta taagtctttt gaaaaaatat aactgcaagc
      721 tcgaaatgcc tgtttattac gaccttgaaa cagaggacca ggttgattta actcaggccg
      781 aatgcaccga ggttgctcga accttctgtg acaagcttaa agcggcagga tattttgtcg
      841 gaatttattg caacaaatac ttcgcgagag acgagcttta tgcctcaagg ctttcggact
      901 atcatttttg gattgccgag tataattcaa gctgcacata taacggcgct tacggaatgt
      961 ggcagtacac ggagaccggc agagtttcgg gaattgatcg agtttgcgac aaaaactatt
     1021 gctactatga ttatccgtca tatattaagg caaacggtct gaacggtttt tcaaagccgt
     1081 cggttgcaaa tcctcaattc agctttaaga ccggagcaaa tctcagcctg aacgaagcaa
     1141 gcaagaggat ttatctgagg aaagaaccgg ggcttacgca agcgcagttc atttcaaaat
     1201 atgtttcata caaggatgta acggtaaagg tgacagcgct tacctcagcc ggcacggtaa
     1261 aaacgggatc aaccgtttcc gccgagggca acggaaaaag cttcggtccg tacacagtgt
     1321 gtcttatcgg cgatatcaat gccgattcaa agataaattc ggcagatgcg ctcttgattc
     1381 ttcgccacgc tgtcgagtta acaacgcttt cgggcaattt gctgatcagt gccgactgga
     1441 acggcgacgg aaaagtgaat tcctccgatg cgcttgaagt tttaaaattt gcggtttcaa
     1501 ggtgatcatt atgaaaatag gtatttttac agacacacat tattgcgacc tcgacctgct
     1561 tgagcaggac agaaagccgc gctatgccta cgctgcggtg aaaagggcct ttgaagattt
     1621 caaggcacag gacgtgcaga ttgccgtttg cttgggtgat ctcgttcatt ttcataacgg
     1681 aaccgaggag agcatcaggc atcttaaaca gatctcgtct cttatcaatt cctacggtat
     1741 tcctacatat cagtgcatgg gaaaccatga taatgaggta gtcagtgccg aggacatggc
     1801 aaaaataaca ggctttcacg tagcaccgac aacggttgag gataatgagg tgaggctggt
     1861 ttttcttgac gcttcttatt ctcccgacgg ggagccttac ggccgtgaag atatcgactg
     1921 gacgaagagc tatattccta agaccgagct tgcttggctt gacgaacagc tcaacacgga
     1981 taaaagaaaa atcgtattta ctcatcaaaa tatagatata aatgttgaaa gccgtcatat
     2041 tgtttcaaat gcgaatgaag tgaacggcat aattgcaaga cacgatgttt cacatgtcta
     2101 tcaggggcat tatcattacg gggcagagaa cattatcaac ggtattccgt atacgacgct
     2161 tcgtgcaatg tgcatcggtg aggaaacaaa ttatcttata gcagaggttt aatatgtcgg
     2221 ttgaggagct tgtacttttt atttttgaaa tggtcggaac cgtcgctttt gcaatatccg
     2281 gagcaatggt tgctataaag aaaagggtag acgttttcgg cgttatcgtt ctcagcgcaa
     2341 tgacggcgct cggcggagga attgtcaggg atattcttat cggtcatctg ccgccgacga
     2401 tgttctccga ctacagatat gtcatggttg cggttattac gtctgtgatt gtatttattg
     2461 tggcatatat ttttcgtgat tcatatcgaa aaagcgaaaa aaccgttgat gcggtcaata
     2521 acgtttttga tgctctcggt ttgggtgttt ttaccgttat gggcgcaaag gttgctatcg
     2581 aatcaggctt cacggttaac ggaatcctcg ttgtttgcct tgccgtggtg accggagtcg
     2641 gcggcggtct gattcgtgat gttatgctca tggaaatacc gtttgttctc aaaaaaagaa
     2701 tatacgccgt tgcctcaatt ctcggaggaa cagcgtatca tttgatgtat attcacaatg
     2761 taaatgttag ggcggcgtcg attattgccg tgctggttgt ttttgcggtc agaattcttg
     2821 caaccgtgtt taagctcgac ttgccgaagg taagattccc gaaagaggaa aaatcaatat
     2881 aacaagtaca tatgaaaaac ctcccctttt tcaagaggag gttttattat atccggtctt
     2941 attttttgaa tgcgttttta attaccttcg ggagaagaac gaagagatac ttaacgagca
     3001 tatcgacgat agccttaaca aagtttgtta tgtaagcact gatgtttgaa acgttatcgg
     3061 tcggaagctc ctgataatct tctgccggct tgttctgtcc aacgtccttc ttaatagcgt
     3121 acttatcaat aagagtaatc tcttgagttt tgtcatcaac aaggtaggat ttcataacaa
     3181 gcttgccgcc gtcaatctct gttgtcatga aacagccgcc caaatctctt gttgaaaggc
     3241 ggaaatcgca gcagcccggg ataggatcaa tagcgtcgta aactctgtat ctctttgtgc
     3301 ctgccgtagc cggaacaatg tgaacggagc cttcgggatt gagtgcaaat gttgtttcct
     3361 cgccattgaa catttctgtt acatactgaa cattatcaac aaccttatcg cccttaacct
     3421 gctttgatct atagtaaaca tggtcgtgac ctgcctctac tacgtcgata tcaagctcgt
     3481 caaagagcgg aataagaacg tctctcatta taatcgcatc aggcttgttg atgttcttgc
     3541 cctcggagta tgatgccctg tgcatgagaa cgattttcca gtccgcatcg gtcttgctca
     3601 tgtcattaac aagccagttt ctctgagact gtgtcatcgg atacatgtcg ttcgtgttaa
     3661 taacagcaaa atgagcgttg ccgtaatcga atgagtagta aacgccgtta acccagtttg
     3721 tgttggactt ggtgttcggg ttactaaggt taaacattga gttgaaaaca ttgatgttga
     3781 gcttgttcgt gatgttgccg tcgtggttgc ctgcaacggg agcaattgtg gtattcatgt
     3841 ttgcgaaagc aaagttctta aaataccagt tccactcgtc gtttgtatcg tcgttaacaa
     3901 agtcaccgag gtttatcatg aactcatagt tcggaagagt tttaacagca ccgtgcattg
     3961 tttcggcggc atgtgcaaag ttctcgtcgc tgcttgcttg aacgtcggca atggaaatga
     4021 aggagaattt gctgtcaccg tcatctgttc tgaatgtgcc gtcaccgctc caaaggttga
     4081 gagatctgtc tcctacacgg tatgtatagc ttgtgcccgg ctcaagattg tcaacggtaa
     4141 ccttatgaca atagaggtcg cggaacttgt aaatattgcc tgccgaatac tctgttgcat
     4201 ttgcgtagct gccgtcaaaa tcagatgtct taatgatttg aacatcgctt cttgtatagg
     4261 tctcggtgta ccagcagaag cctcttgaag tttgagaatc gccgttgaaa gcacaactga
     4321 ctctcgttac cgtatctgtt gtttctgccg atgatgctat tgccgaacag ccgaagatca
     4381 tgcagataca aagaattacg gaaataattt ttgtagaaat ctttttcata aaaaacctcc
     4441 tgtgagtgct atattgtcgg gaacaatgtc cctattatta gttaaattat acaacaaaaa
     4501 agcaattcag tcaacgtaca aaatcggcac ctttttttgt gtaaaacgct cataaaaccc
     4561 gacacaatcg tttgaccgtg tcgggtgaag ctttaatgtt atgctttctt tttagaaaga
     4621 tttgcaaagc tgaaattgat atatgctttg ttgagcttgt gctcgtcgtg cttatagaac
     4681 caatttacaa gcagaccgcc gataatnnnn nnnnnnccga ttgcaccgat aataagcccg
     4741 ccgatgacat cggtcggata gtgaacgcaa agataaattc tcgacattcc catgaatatc
     4801 gcaaagagaa acgctaacca actgatcttt ttatttgtaa cgcagaaaag tcccgttacc
     4861 attgccattg ccgaggttgt gtgacccgac gggaacgaac ggtcgctttc aacctgcgct
     4921 cctgcattca gccaccattg ataaacgggg ctgtcaagat gagtgtacgg cctcggacgt
     4981 gagacgaggg gcttgattgt caggtttgta aaaattgctc cgacaataat tcctgcgagc
     5041 attgccgagc ctgcttttct cgtctttttg aaaagcatga ggataagtga aatgataatc
     5101 agcgcaattc cgtcatgtcc gagcgttgtt atcgcattga aaaacacatc aaggaatccg
     5161 cctgccgcac tgtcatgcaa tctatggaaa aagtcaaaaa ttgcaaggtc gaagtcggca
     5221 aaagttgagt tgagccaaga ggcaactgct gttacttcca ttatttaaac ctccaaaaaa
     5281 agatatgatt tatattagca ttttttagga aataaatcaa tgttattgaa aaataattta
     5341 ttttttgtta ttataatttt gcccttattc taaattttta cgggagggtt aaacaatgat
     5401 ttttataacc aggattattt cagccgttat ggcggtaatc atggcacttg tcacattgaa
     5461 cggcaccgtg acgaaaagct cggattctcc ttcttccgac aattatccgt acacgcttga
     5521 ggatatgtcc gttgtttatg ataatgttga cgaaaacggc aattattatc cccttgtcgt
     5581 tgtgcccggc atctcccatt cgataaccta tgttgttgac agggattaca acagctcagc
     5641 cgccgaggat ccgtctcgtc cgccgataaa aaaggacgcc ttcggcaatg accttcagag
     5701 cggaaccgtt atttttgatg ttcccgaaat tctcaaaacg gttgcaagca atcttatagc
     5761 accgcttgtt aaatcggtca ttgctcaaaa ggactgcgga cttgttgatg caatcggaaa
     5821 ggttgccgat acagcttttt cagcacagaa aactttgccc gacggaacct ttgcgaacaa
     5881 tgaaatcggc cttcttgaat ttaacgggtc ctttgccgat tttgcaactc cggccgatgc
     5941 cgaaacgcag agctatatgc aatatcagtg tgactatctc tatagaattc ttccgcttcg
     6001 tgccatttcg gatataatag gcgaagataa tcttttcttt tttacttttt ctcttttcgc
     6061 cgacccaatg tcatctgctc aacggcttga caggtacata gatatggtac ttcaaaagac
     6121 gggagccaag aaagttaatc ttcttccgat aagtctcggc ggtacagtgt tcacagcgtt
     6181 ctgtgaccaa tttaccgaca ccgacaaggt taatacaata gttaatgtcg tccccgttct
     6241 caacggaacg cagtcggtta ccgatatgtt caaccgtagt tttgatgttt cggcggaatt
     6301 ctggtacaac gcgggaatcc cgatgatgat ttccgagttc acggagtacg gcgagcttat
     6361 cggtcacgcc gtcaacttct tgcttcgtgc actccctgcc gaggttaacg ccgcaatgct
     6421 gactaaaatt tatgatattc tttttaacaa tctttttgtc aacacaccgc agatctgggc
     6481 aatggttacg aaagaagctt atcccgaact tgcagaaaaa tttcttagcg ataaggagca
     6541 ttctcccgtc aggtcaaaga ccgatgcgtt ttatcaggca cagcttgacc ttgccgacaa
     6601 tataaagaaa atgactgcaa acggaattaa gataaatata atttccggct acggacttca
     6661 cacaggtgat gcacggtata atttctttca tgttactgcg aatgcggaca atgtgaacgg
     6721 cgacggagtt atcaatattg aatcaacagc tctcggcgca acggcggctt tgcccgggga
     6781 aaagctctcc gagggaggca gactttctcc cgacggtgag attgacgctt caacgggtgt
     6841 tcttccggac aatacatggt atttctacaa tatgcatcac gaggatgcgg cgaacaatgc
     6901 accggttatc aatcttgcgg ttgcgctgat gtattcgcat gaagttgaaa atgtgaaatc
     6961 aaagccggaa aaatatccgc agtttaacgg caattccgac aactggttta tccgccgctg
     7021 gagatacaat gacatgaaaa atctctatgc agactatcag gcaggcaagc ttgattggtc
     7081 acaggagacc gtcgaggaat gtaaggcgat aatgtatgac tgtgagcgtg tgatgcgtgc
     7141 gacgattgcc gacgcagagt tttgcaagga aacgacaagg cgagttaatg aattctgtgc
     7201 gaaacaaggc aagataagcg aggaaaacgc aaagctcctc acgcaaaaac agctcaatga
     7261 gctgccggaa tgggcaaagg ctttgacgaa gataataaca tttgtcgacg acaccgtttt
     7321 caaaatttac ggcgacaaag catattcgga attttggaag acatatttca aagaaaagat
     7381 acagcaccgg gtttggtttt cggtgctgta tctttttata ttgcagttgc ttctgcatca
     7441 attatgtcat caactttttc gccgatatca aaattatttt ctacaaacat ctgatacgct
     7501 ctttttgcaa tggcttttgt ttcggcaaaa tctacaccgc cgttaattac ggcaccgact
     7561 ccgggaacca acttgccgag gttaattaaa cccttttccc cgaattttgt taaaagtcta
     7621 aaaccgacct tttgatttat ttttgttaaa acttttcccg gtattttttc aatcattcct
     7681 acggcaactt tttgacctaa ttttattccg aactgcttag ctatttcacc gaccgataac
     7741 cctgcaaggc atgcatagac taatgtttga acttggtcac ttgagatatc atagccggcc
     7801 atatatgcgg tgcaggctat cattctcatt tgcacataaa ggacgcttcc gatatttgca
     7861 ggcattgtaa ccggtaaagt tattgcccct ccgaatcccg taatgaatcc ggatgttgtg
     7921 cattttaaaa cttgtttttt cagcatatct tttatggctt cttctttaga agcgtgttta
     7981 cttaaataat cgcccgcaag tcgatctatt gaaggactga tttttgatat tccgtttgtg
     8041 gctttttcat aacaggtgtc aagaactttt aaaatatctt cctgagaaaa tttaattttg
     8101 gttttcatta aaattttatc ctccatattg acattcactg aatgtcatgt tttggcctat
     8161 tataacatat acttttcttg ttgactactt ttcttgttga caagcactaa atgtcaaaaa
     8221 ggagaaaaaa tgtttatcaa taaaactgat gagggcttta ataatttctg tggaagcaga
     8281 atcgccgaaa gaagaaaaga gatgaaaatt tcccagcgag aactcgctga cagacttcag
     8341 ataattggac ttgatgtgga taaaaacgct gttcagcgta ttgaatgcgg taaaagattt
     8401 gtgaccgata tcgagctaag agccttttca aaggcactcg gactttcatt tgatgaactt
     8461 ctatcagctt gaaaaacagg ctgcaccgaa gcgattgctt tgatacagcc tgatattttt
     8521 ttgacttatc ttaaatgaat cgtgtcaaaa aacgagttct ttgctttgcc gatgagcgtg
     8581 gaaatcaaaa tacagattac acacacgccg acgtcggcga aaaccgcaag ccaaacagga
     8641 gcataacctg ccgctccgag gatgagaact atcatcttta taacgattgc aaatataaga
     8701 ttgaaatgaa taatgtttat tgttctcttg aaaaggttgt gagccggtgc gagcttgtcg
     8761 agcctgtcgt tcgtgagaat gacatcggcc gcttcacttg ccgcctgagt gccgagaccc
     8821 attgcaacgc ctgcgtccgc ggaagcaaga acgggagcat cgttaattcc gtcgccgacg
     8881 taaacgacct tgccgtactc gttcttgatt tcctcaaggt ggctgagctt ttcttcggga
     8941 agaagattgc aatagtattc gtcaagattg attgcttttg caacctcgtc ggatgcagtt
     9001 tcgttgtcgc cggtaagcat tataatgtgt tcaacgcctt gatcacgcag atgctcgacc
     9061 atgtcctcaa cgccctttct gagcgcactt ctgagggcga tcgtgccgat gattttgccg
     9121 tcaaaaacaa cgcaaacgtc gccgtccgtg tagtcgggag tttcgatacc ctccgtctca
     9181 ag
//
LOCUS       DAWBSW010000071         9159 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1566_length_9159_cov_2.738357, whole genome shotgun sequence.
ACCESSION   DAWBSW010000071 DAWBSW010000000
VERSION     DAWBSW010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_9159_cov_2.738357"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    841..850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3505..3514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2540 a         2093 c         2031 g         2475 t
ORIGIN      
        1 tttatcttct gtgccgacgt tgttttggaa acaggacgga tataatcgaa ttcctccgtt
       61 tcgtcaatgt tcggtaaatc tccggcctcg tcacttccgg cattgagcgt gtaaagtttg
      121 atgacagatt ccggctgacc tttttttatc atcgccgaaa gagttcgaac aatcgcttca
      181 cagccgtggt tttcgcatcc gccgtgggaa tagaaagcgt atctcattgc attcactcct
      241 caataagctt aacatcaacg tccctgagct gtacccagcc ccattgtctc ggccatgttc
      301 gtgccgtgcc atcggcatgg agaacctcaa acacaaatgc aggataaaca taatcaaggt
      361 tttcgctgtt agcgtttgat gttccgccag agagaattat ggtgagcttg tacagaccgg
      421 gtgcaaggtt atgcttgttt atcgcaaccc caacatcata ttctccgatt tcgttgatat
      481 ttgcaaaggt cttggtgaat gcaatttcaa tcggtgtttc atcagcctgc caaacaagaa
      541 ttctgaaact catgtcaccg atattcggaa ccttggtctt gattcggagc ttaaactcta
      601 tcggctcgtc ggcagcataa acgcaggatt cattatgaag aacatcaagc gaaagaattt
      661 cagccttgtc aaggttgtaa ccgggaagcc ttgcaagatc gctgaaatta taatgattca
      721 gtttttccat gtaagcttcg ctgttatata ttctgatgcc gccttcaaca tctccgtcat
      781 aaatcacctg tccctctttg aggacaatta ctcggtcgca gagttggcga atagtattca
      841 nnnnnnnnnn tgtcgccgac tgcaagaacc tcatcaacaa gcagaatatc cggctccaag
      901 tgagatgcaa cggcaaaagc gagacggaca aacattccgc tggaatatct ttttaccggt
      961 gtgtcaatga attttcctac ctcagaaaac tcgagaatat cgtcaaattt ttcatctatt
     1021 tctgctttgg tcattccgag aatcgcaccg ttaagataaa tattttccct gccggtcaac
     1081 tcgttgttaa agcctgtacc aacctcaagc atagaggaga tttttccata aaattcaatc
     1141 tttccgtcgg tcggctcggt tattcttgaa ataatcttaa gcagggtcga ttttccggca
     1201 ccgttttttc cgataatgcc gaccttctcg ccttgcttaa cgtcaaaaga aacaccttta
     1261 agcgcccaaa aatcctcctt ggcatttgcc tgaatcggat gaataacgtt atgagccatg
     1321 agcttcaaac gctcaattct gctgtccggt ctttttataa cctgcttgat aacgtatttt
     1381 ttctttatat ttgagatatg taatgcaatc ggttgttcgc tcatatttac tcctccgtca
     1441 aacgatatca acaaagtttc tctcggttct tctgaataat ataattccga aaaccgctaa
     1501 cagcacaagc catgccattg caaccaagaa tgaaagtgta tcaaaggaca tatcaccgat
     1561 aacgcaccat ttgaatgccg aaatcatacc ggtaaccgga ttaagcgaat agatgagtct
     1621 gtatttttcg ggaattgagg acatggaata agaaacaggg cacgcatatt gcaaaattga
     1681 aagtgcaaac ggtacaattt gtgccatgtc acgccattta acattaaacg aagaaagaaa
     1741 caaacctgta aaaagcccga ggaaagcgtt gagcaaaaga aaaacaggga aaagaagaaa
     1801 acgtacggtc gggtaatatc cgacaatgag catcataacc acaagaacaa caatggaaat
     1861 catagagtca aaggcagtca caaagcatgc accgatcggc gaaagtattc gcggatagta
     1921 gacttttttc atcaatgccg catttgtgat gaatgttgac gacgtttgga gaacattgcc
     1981 ctgaaaaatc gcccatggga tacttccggc ataaaccatt ataaaataag gggcatttcc
     2041 gtcagacgaa aggccggcaa gagttccgaa aataaaggtt gagacaaaca tgctgattat
     2101 aggagcgata accgcccaac cgagaccgat tcttgtctgc ttgtatctta ccgtaatatc
     2161 acgttttgca aggttatatg caagttctct gtaattccag atatctctcc aatactggag
     2221 attttttccg ttaggctcaa taacagtttt cctcaaatcg aaaatcctct cattaatttt
     2281 ttgcacatag aaaattatat catatacatt atgtaatagt caatacatga aagtcctgca
     2341 acgtcaaata aaaagcttag aaaaaacaat attcgtggtt taaatatatt gactttttta
     2401 aatgattttg atagtataaa ttgattaaga ttatttaaag caaaggaagt atcatatgct
     2461 tttggacaaa acaattcata ttcccgattt gggagacatc ggtgcagaca gcggctttca
     2521 cgttttggac gctactgagg acggaaaatt ttccgtcggt aagaacggag ttgtcgatat
     2581 agtttcgacg ggtgacagca aggttgagtt cgtctctttc ggctcacaca ctcttgcctg
     2641 tgtaaaatcg gcaatcgcat accccgtata ttatcccgtt gcgcccgtta ctcttgagaa
     2701 gcctgttaag gctgttctta tggatctcga cggcacaacg gttcgaagtg aagaattctg
     2761 gatctggata attcaaatga gcattgcaag ccttctcggc aatccgaagt ttgagcttga
     2821 agaagctgac cttccgtttg tttccggtca ttctgtttcc gagcatcttc agtattgtat
     2881 tgataaatac tgtcccggcg catccctaga caaggctcgt gatttttact ttgagcacac
     2941 tcacagagag atggaagaga tcatggcagg cagaggaaag gacggcgcat ttactccgac
     3001 ggagggcgtt aaggactttc tgtatgagct taaggacatg ggcgtaaaaa tcggtcttgt
     3061 tacctcgggt ctttacgaaa aggcatggcc ggaaattctt tcggcattta agactctcgg
     3121 catgggtgcc ccgaaggatt tctatgatgc aatcatttcc gcaggcttcc cgctcagaaa
     3181 aggctcggtc ggaactctcg gcgagctttc cccgaagccg cacccgtggc tctattccga
     3241 aacctctatt gtaggtctcg gagaggcttt cgctgaccgt aatcacgttg tcggcattga
     3301 ggacagcggt gcaggtgttt gctccgtccg tcttgcaggc tacacgacta tcggtatcgc
     3361 aggcggcaat atcgttgaca gcggcacaaa ggctgtttgc aattattacg aaaacagctt
     3421 tgccgatatt gcggcatatt gcggaaaaaa caatctgcgc ctttcggatt acgtttacaa
     3481 atacgagggc gatgagataa aagannnnnn nnnnggtggg gctgtgccgg ttgccgatct
     3541 cgttctggac gatgagcact ggccgaatgc cgccctgttc cgatcccacc accggtgaaa
     3601 acagctttac cgatatttta aaaattataa agggatgatt taattgaaga aaagaatcac
     3661 ttccgaaaag cttgttcacg cagtaagctt tgcacttttt gttcttatgc tgaccgaggc
     3721 ataccttgtc catgaggacg agatcaaggc aagcgttgca agactcaaga caatcatcga
     3781 gaataaaaac aaaacaaaag aagccgaagc actcaagcag acggaagctt cggaaaaagg
     3841 agaacagaca gatggacaac aaaccgcaga agattaactg ccatttcatc tcaaacaccc
     3901 attgggacag agagtggcgt ttttcagcaa gaagaactca gtatatgctc ggctatatgc
     3961 ttgatatgct gacggatata cttgacaagt atcccgagta cagacatttc catcttgatt
     4021 cacagactat gcccattcag gactaccttg aggcatatcc cgagaagaaa gagcttatga
     4081 aaaaatacat atccgagggt cgaatcggtg tcggtccttg gttctgcctc cccgatgaat
     4141 tcactgtcgg cggcgaatcg cttatccgta acctcctgct cggccacaga atcggcaagg
     4201 aaatgggcgg cataagcaag acaggctatt caccgttcgg ctggggtcag atttctcagc
     4261 ttccgcagat ctatgcaggc tttgacattc actttgcctc attctatcgc ggaatcaacg
     4321 agtattgcgc tccgaagtcg gagttctact gggaaagccc ggacggaaca agaatttatg
     4381 cttcacgtct cggcaagcgt ccgcgttaca acatttggta cattgttcag cgtcctgttt
     4441 attacaatca gcagaacgag aataaccgtg acatggtttg gaatgacaac aacggtatat
     4501 tcaagttcat tgattccgag aacaaaaatc ttgactatca atacgctcac ccgttctatg
     4561 agtaccataa ggagaacatt aagccccgtg cggagcaggc aatcaaggag caggacaacg
     4621 attggacaac gtctcaccgt ttctggtcac tcggccatga ctcatcatgc cccgatgccc
     4681 gtgaggttga gatgattaaa gaccttgacg cagctctccc gaacgctgtt gtttatcaca
     4741 gctcactcaa agaaatggag cagggcatca ttgatgagtt tgatccgaat tcacctgttc
     4801 tcaagggaga aatgcgtgat ccgtacacca aaggcagcgt cagcagcatg atgggctgga
     4861 tcatttccgc aagaacctat gttaagcagg caaacttcaa gacggaaact cagcttcaga
     4921 attacgccga gcctatggcg gtttttgcgg cactcagcgg agctccgtat cagaagaact
     4981 tcatcgacct ttcatataac tatcttcttc agaatcacgg tcatgacagt ataggtgcat
     5041 gcggacgtga tgtagtttat gaggacgtta tctatcgtta caggcaatca agagagcttt
     5101 caaactgtgt ttatgagcga gctttcatgg acgttgcagg cgatattaag cttgacggct
     5161 tcagccgtga cgatgcagcg atcgttgtat ttaacccggc tccgttcaag agaagcgaag
     5221 cggtcaagat gacggttgag cttccggcag agtggaaagc ggacagcttt aagattaccg
     5281 atcccgacgg aactgttctt acctatcagg taattacgac aaacaagaac tcggctcaga
     5341 tcattcagaa tccgaacgac gttgccaatg ttcttcatac tcagcagtac acgattgagc
     5401 ttgacgtcaa agatgtaccg gctatgggct ataaaacgct aaagcttcag ccgctttatc
     5461 atgtccgcgc aacaacgcct aagactatgc tcaagagaaa tcaggttatg gagaacgagt
     5521 acctgagagt ttcgttcaac tccaacggaa cttatgatgt tcttgataag gagaacggca
     5581 agagctatgt tggactcgga tatttcaagg ataccggcga aatcggcaac ccgtgggagc
     5641 acttcacccc ggagaacgat gaaaccttta caactcttaa cgagaatgca aagattacgc
     5701 ttctccacga gggcgagctt gccgtttcat acaaggttgc catcgactgg gcacttcccg
     5761 agggaagaaa cccggacgaa aagacacgtt caaagcacct caatccctgc aggatcgaga
     5821 gcattgtcac attgaagaag ggctcacgtt atcttgaggt tgaaacaact gttgacaatc
     5881 agagcgagga tcactacctc caggtagcat tcccgactga catcaagtcg gaattctcat
     5941 acgctcaggg tcagtttgac gttgttaagc gccccgttgc aaagcttgac tattcgcttt
     6001 atgacgagat accgatgacc gagcagccga tgaactcctt cgttgatgta acagacggca
     6061 aggtcggcgc agctctcctc aatacgggac ttaaagcata tgaagcctca gatgatgagt
     6121 gcggcacggt ttatctttca cttcttcgcg ctttcccgct tcgcatctgt gttacaagcg
     6181 atatgcagga ctacagcgca agcgataagg gctcacagtg catcggcaag aacacctacc
     6241 gttatgcttt catgccgcat aagggagact gggaagatgg caatgtttgg ggcgagagcg
     6301 aaagattcaa tcttgctttc ggcgtagcac agcttgctcc gacagaccac ggaaagaacg
     6361 accttgttca ttcattcctt gagcttggca cagaaaagct caacgtcagc ggcattaagc
     6421 agagcgagga cggtacaggc tatgttgttc gtctctttaa cccgtctgac aagactgtaa
     6481 agacaaatat tcgcctcaac ggcggtatcg ctccgattga aaaggctcag tcgcccgttg
     6541 aaagacagca ggcagagttt gaactcccgc cgtattccgg caagaagtgg agcgaggtta
     6601 agctcgtaag ccttgaggaa aaggatgttg ctccgcttac ggttgatgag aacggcttcg
     6661 cggactttga gattacaggc aaaaagattc ttacgattaa atttgtcggc tgataaatta
     6721 caactgaggg aacgccatta gtccggcgtt ctctcgggca agctgattgc ccgaactcga
     6781 ggaataaatc aaacaatgaa acatctacgg aaatttatgt ttacggcggc ggctctgttt
     6841 gccgttctca gtgttatttt cctaatttcg catattcacc atgacaatca ctattctttc
     6901 acctgtttta taactttttt aacctgcttc taccattttt catacagaat tcttgtttcg
     6961 gaagttgttt caaaaaagcc gataagattc aactataaaa gcggttggtt tgcaccgaaa
     7021 aagtttgaag agtctgtttt caaagctctc agagtaaaac gttggaaaga aaagcttcca
     7081 aatcttttgc cgcagacctc ctcatcaaag gatttcccgg ttgaaacgct tatcaatgag
     7141 acctgttcgg ctgaggctat acatgagatt aacatcgtgc tcagcttcgt tccgttcata
     7201 gtttcgctgt tttacagtga gcacgaatgg atttttcttg ctacgggctt ggtcacggcg
     7261 ctggttgacc tgtgcttcat tatctttcac aggcatttca ggccgagatt gattaaattg
     7321 atgaatacaa aagcataaaa gccatcgaac aaaactgtcc gacggctttt gataaatctc
     7381 atgctgacga attgcgcttc atggcgcata ataaagatac aaagggcggc actgccgata
     7441 tacggttacg ctctcgaatg aaaggttaaa gaataaccgc tcgttttctc aggacagggc
     7501 ggttatttct ttttatatgc aagaactgca acgaacacag tcataagtcc gatgattaca
     7561 agtgcgaatg tgaataaatt ctcatatgta acgtattgca taaaatcacc ccctgacgag
     7621 ggcaaagagt gtaaccgcct accgcattgg tagtgccacg gagataatta tatctactca
     7681 ggttgattta taaattcttc tcgaggcaca caagctgtac gccgtcaatg ccgttgaaca
     7741 cacatggaac tatcgaaact tcggtatagc cgagcttttt atatagactg cgtgcggcgg
     7801 tgttcctcgc attggtgtcc attctgagat aaggacatcc gttttcaagc gcatattttt
     7861 catagaaatc gacaaatgcc gttccgtagc cctttccctt agctttaggc gaaacgacaa
     7921 gcgcatgaag caccataacc tcatcatcgg gtgcatcggc agtccagctt gcatcggcat
     7981 attcgggcac ctgttctttg tttatcctcg cggcagcaac tattgttccg tcaatttctt
     8041 caacaaacat atctcctgcc gcaattgcaa tttctgctgt cttccttgtc ggataaatat
     8101 ttcttatcca gcctattgta accagccctg cctcttccga gtcgtgaatt tcatcataaa
     8161 tttcactgat acggtcaatg tctttctttg tagcttttct gaacataaat ttcatgcctt
     8221 tcaaaaaaat cgcccgtctg aaacggacga ttttctatat ttatcttacc aaatcgtcaa
     8281 gcttttcttt catggcctgc tcggtatcac ggtaatattg cgttttagga ttaggatgct
     8341 tgttttccgc ccaaagcttg tccgcctgtt ccttccatgc ggcggcgaat ttatcgcact
     8401 ttgcgcaaag atgttcaaca ccttcctgat tttcaaggtc ggtcgatttt gcgcctgtct
     8461 gcttaatcat cttcctgaga tattcggggt tctcaagcat cgggcacgga cgcagatggt
     8521 tatcattgaa cggctgattg tgataatagc tcataaacag cggatttctc aaagcctcaa
     8581 gaatcgtatg tgttctgata tttgaatcag aaaaatgaat gaatacgcaa ggctcaatat
     8641 caccggcgga attgatgtgg aaatagtttc tgccgcctgc gatacaaccg ccgacatatt
     8701 cgccgtcatc ctggaaatcc attacaaata acggcttgcc ggttttgccg tttcgcatct
     8761 ttttgagcca gaaatacata tgcgttctct gctcaggggt gggaataagc tcctcaacag
     8821 ctcccgtgcc gaccggcata taattaaagt aaagtgcaaa ttttgcacct ttttcaatca
     8881 tcaaatcaat aaattcgtca cttgttacct tatcgacatt ctgcgaggtg tagcaaatcg
     8941 aaagaccgta aatgcactta ttctttttaa gaagctccat tgctttaatg gttctctcgt
     9001 aagctccctt gccgcggcgg gaatcgtttg actcctcaga accttcgatg ctgagcgcaa
     9061 gagtaagatt gccgacatcc ttcatgtcgt cgcagaattt ttggtcgatc aatgttgcgt
     9121 ttgtataagc aaggaacgtg cagtttttat gctcacggc
//
LOCUS       DAWBSW010000072         8992 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1593_length_8992_cov_2.960613, whole genome shotgun sequence.
ACCESSION   DAWBSW010000072 DAWBSW010000000
VERSION     DAWBSW010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8992)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_8992_cov_2.960613"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1985..2016
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2468..2477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2446 a         2035 c         1823 g         2646 t
ORIGIN      
        1 ttgcaaggtc tccttcggcg cagagccgaa agactttgag gttttgaatt gcattcaaaa
       61 aaattattgc aggctgaaat tcagtccgtc tgttgaaacg gctgtaaaaa atgactcgac
      121 aaatccgaag cgcaagcagc gagaagcaaa gaagttgacg gctcaaagcg ggatcggaac
      181 aaaaagccag caggctttac agcttcagcg agaggaaaac aagcttaaac gcaaaactct
      241 cagcaagcaa cagcgtgatg ccgaaaaaga atggcggttt gagctgaaac agcagaaacg
      301 aaaagaaaaa cacagaggtc actgatctct gtgttttttc gttctgttat gcaagagttt
      361 ctattcaatt tccttgagcg tttctgccat attgattttg tctatcttga gccttatagg
      421 cagggctgaa atcagtgata tcagagcaat aatgacaatc gtaacgaggt agctccacca
      481 tgcgatcgag ctgctgtaaa cgatgttgtc aaccgagata aagccgacca aaaccttatg
      541 aaggaaaatt ccggcaatca atccgatcac tgcaccgtaa acggttgaca tgacactctc
      601 acggataaca taaatcaaaa cctcatgatc gctcagaccg atgaccttga tatttgcgat
      661 ttcccttgtg cgctcactga ggttgacatt cgagtttgta tacataacta tcaacgcaag
      721 catacaagcc gagagaacaa acatcataac aacggcaaga attctgctta tagaaacatc
      781 aaccgagctc gccatttccg acgcggtcga cgcacccgtt acgatatcct ccttgagaaa
      841 atcggatgca aaatttttga tatcatgctc atcgacatat gactttgacg taacgattat
      901 atatcggcac tgcggcggtg ttccgaaaat ttcttcatag ctttcatcag tcatgtaaac
      961 ataatgattc gtatagtttc tgactattcc gctgacggat gcgctgtatg tcgatccgtc
     1021 cgtggatgcg agatatatct caccgccgac ctttattccg agatcgttcg ccattttttc
     1081 tgttataata acattaccct tgccgaaatc aaggtcgccg ctgactgttt tgagattgac
     1141 ataatcgaaa aaactgtcgc tgttcttcat agcgatgatg ctgacggttc gtttaacctg
     1201 tgcctcgctg tctgccgaag caaatgcgct cgtgttgctg aagagtgccg cattttccgt
     1261 tcgctgatca ctctggattt tcttaagcac ctttgcgtcc tcctgctgag gagttctcag
     1321 tacaaacaat atatcatact taaatattcc gtcatcggca tactgcgcgt ttgaaacagc
     1381 cgtcgtcgaa tttatcaatc cgagtgaaga aagaatcagc gcagtacagc aggcaatgcc
     1441 aagcgtgccg gcggtaactc tctctcggct acgggaaatt gttcgtgcaa caacaaccat
     1501 tccgtagctc atttttcccc aaagaccggg aatcttttcc agaatatttc tgcggttata
     1561 cgcaatgttt tttggcctca tgagcgttgc cggattatgc ttaatttcat ggatcactgc
     1621 aatcaaggtt gccgcaacaa tcaatatcaa tgaaatcgca aatccgaaga aaacgtagtc
     1681 tgcaaggaaa accgtgccga cattcgggac actgtatgtc atgttgtata tggagcaaat
     1741 aacaaacgga agcacgcagg ttcccataac agttccgata aagatgccga ttatccatgc
     1801 aagtacggcg tagaggataa acttaccggc aaccgccgaa ttagaataac ctaacgcttt
     1861 aaaaatgccg atttcttttc tttcctcctc aacatttttt atcataataa caagacacgc
     1921 tatcattgct gtgaggaaaa agaccatcgg aaaaactgtt gacattgatt ttatgttttc
     1981 aagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttc tcatgcccga aaaatcgttt
     2041 ctcggcgttg ccgtccatct gaggtttgca atattgccga gcgttgtctg aaggcttttc
     2101 agctcgctgt tcaacgttcc ggatttactg tcaatgtcat ttttaagctc ggtgtattcg
     2161 tcgtcaaccg ttgaatagcg gctttttgca acctttaaat catactccgc ctttgaaatt
     2221 tcaaccgaac cgtttcttga agccaaagta tagctgattt gtgcctctgt aacacgtcgc
     2281 ttgagtgttt ccaactcgtt tgcttcgctt tcaacctgag caattagctt tgtaaggctg
     2341 ctctgtccct ttgtcagctc attcattgaa gagacatatt tttcaagcgt tgcggaatcg
     2401 gcatcatagt ttttctttgc cgaattgtaa ttcgacaagg catttgaata ttcggtttta
     2461 agcacttnnn nnnnnnnctg ttaagccttg ttcttctgct tattgccgca atcttttctt
     2521 cgtttgtcgc cgatgaatcg gaaataatgc tattctgttt ttgtatatcg ctttttattc
     2581 cgtcaacaac ggaattttga gcctctactc tttgagtata agatgaaacg ctcgagttaa
     2641 gagatgaaat tgttctttca agctcgtcta tctcagaggt aagccttgaa attcttgatt
     2701 catagtccga gattttattt tcacgctcgg atatttcatt attttttgca tcgagtgcgt
     2761 tctttttgct ttgataattg ctttcaagcg aatccagttc cgttttggct gttaaatgtt
     2821 ttcgattaag gtcatcgtag agctttttca gctcgctgta acccttgatc tccgagcttt
     2881 gactgtctct caatttaacg tgtgtattat aatcagcctg aagcgttgta agctgctttt
     2941 caaggctgtt caatgttgac gaagcggatt ttatcttgtt ttcatttgtc gttttcagtt
     3001 caccgagtat cttatcctcg ttatcgacat actgcttaaa ttctttaatg gaagcctctt
     3061 tttccttgag ctgtttatcg ctcgaatcgg aaaatgcttt tatctccgct tgcttatctt
     3121 caattttttg attgtattca tttttcagtt cgacgagctt cgagtcaatt gcctcttttg
     3181 atgcctctcg gattttttcc gcaatcattt ccgctctgtc cgagtattgg cttgaaaaag
     3241 tatcgagact gtcggaatac ctcatcttga cggcaagagt gttgacctca gatgaggaaa
     3301 acgcctctgg caaaacataa atatatgagt caaggctgcc cgaaccgagc ttagtctgac
     3361 ctctgtcaag tgaaatgtat atcggagatg tgaccgttcc gactatgaca aatttattgt
     3421 ttttcaacga aactccgacg ctgctgtcgg catccgttat gttcagcgtc ttaccgattt
     3481 cgatatcgtc ataaagctct gccgttttct catcaacaac acactccccc gccttctcgg
     3541 gaagtcttcc gtctttaagg tcaagggcgc tgaggaagga cggatcgctt tctccgtcaa
     3601 ggaatttctt ttccttttca acgtcaaatg agctgattct cagaataagc tccgtaccgt
     3661 ttttgttgac aagggtctcc ctgccgagcg tcgcatagcc gtcgatatag cttgatgcga
     3721 caacccgagt tacatttttt atatttttaa ttttctctat atcttcgttc gtaaaaggaa
     3781 ttgacgatga aacataaacg tccatcaaat tattcctgtt gaaatactca ttagcctcat
     3841 aattcatggc ggggcttgcc gagtttattc cgacgaaaaa gctcattccc agcgcaataa
     3901 taaccgctac ggagagaaat ctgagcttgt tggcggtaat ggaacgaaga gcttccttaa
     3961 tgagggtttt cgtcattacc actctatcct ttcaaccggc atcggtgtat cgttgatttc
     4021 aattttgctg attttcccgc tcttcattga gataattctg tccgctatcg gcgcaatggc
     4081 atggttatgg gttattatta caactgtcat tttcataatc tccgaaacct ttttaataag
     4141 attgagtact tggattcccg tttcatagtc aagcgcaccc gtcggctcat cacaaagaag
     4201 aagcttaggc ttttttacaa gtgcccttgc aatcgcaact cgctgctgct caccgccgga
     4261 aagctgcgac gggaaaagat ttgccctttt ctccagtccg accaatctca gaagcgccat
     4321 gcagtcaacc gccttgtcgc tcagattggc aacaagctca agattttcaa ccgcagtaag
     4381 gttcggaaca agattgtaag actgaaaaat aaatccgacc tcctgcctgc gataaagcat
     4441 aagcatgcgc tttccgaaat cggttattat cttgtcatcg accgcaatct gaccctgtga
     4501 gcatctgtcc attcctccca acacgttgag aagcgtggtt ttgccggctc cgctcggacc
     4561 tacgacaaca cacagctctc cctgttcaat ggaaaagctg accccgtcga gagcatttac
     4621 ggtctccgag cctgatttat atcttttata cacatctgca aattcaatgt aacccataat
     4681 acaccgcctt tttaggcatt ataaaaatta tccatagtaa ttatacaaca cccatgctct
     4741 gtttgcaaga aataacttga tatgatataa aatattttta tacaaatatt attttatgaa
     4801 caaaaacact tcctatattt gaatgattac agtacggttt ttaaaatttt ccttgatttt
     4861 cctcaccaaa tactctataa tgtatctgta atttattaaa cgtataattc tctaaggagg
     4921 actcttaatg aaaacgttca aaaaagtctt ggcacttgtt cttgttgccg tgctttgctt
     4981 tgcgcttgct tcgtgcagca tggttgacga caccgacatc acaaaggata acatcaaaat
     5041 cggtgttgtt cttcccggac aacgcgacga tgcggatgat tatgttcccg acctctcgac
     5101 acttcagtgt gtctccgcta taaatgatct tatttccttc ggttcgggta tcaataaaga
     5161 cagattccgc tatattgaac atgttgatcc ttcaaatgaa caggctgcat atgacgccat
     5221 tcattctctc acaaatcagg agtgctcaat aattttcctt gcagatccga gctatatgtc
     5281 cgcggttaac aagtacgttt cgaattcatc ttccgatgtt cagaaatcta aggttcaata
     5341 catcgtattt aacggtgagg ataacggcaa gaatatccat tcctacacag ctaacatctc
     5401 cggcgccgtt tacctttccg gtattgcggc aggtctcaag gctaaggaac tcaaggttcc
     5461 gaaaatcggc tatcttctca agtcggccga caaccttacg ctcctcaatg cttttgctct
     5521 cggtgcagag tctgtttact cagaggttaa gatcgatgca agagtatgca aggacgttaa
     5581 gtcggactgt cacacgctcg ttgcaaacgg caatgtcgtt atcgcatcag acttctacag
     5641 ccgggaaatt gcagataacc gtgaggacgc attcttctgc ggattcggtt cggataagct
     5701 cgccgattgc gagggcttca tctgtgctcc gcgctatgac tttgctcagt attatctcaa
     5761 cacgatcaac gaagttcttg actatgacaa tgttgaatat gacaagaacg gcaaagaaat
     5821 tgacaagtca aatgcacttc cagagattcc cgcatataac ggagactaca agggcggcgc
     5881 aacatatctt tcaagactcg gcacggccgc agcgcccggc acgcaggaga ctgttgacaa
     5941 atcagaggca atgatcctca acggcacctt ctctcagaaa gtcagcgcaa ccgttccgat
     6001 tgcaggaatc acacttgcaa aataaaaccg aatataaact aaggcggagg tcattgcgat
     6061 ctccgccttt ttagatttaa acttttgata ttaggtaaag tatataaatc atattttatt
     6121 ataaaaatta tcagctccgt cagacaagcc gattgcaagc cctcagatcg tataggtttt
     6181 caaaatatcg ccgtcaaaat ctttccaaag gaacacaccg ttttcaagcg tcatatatcc
     6241 gtggcggctg ttctccttag gaattgaaat cgaaccggga ttcatataaa tatagttttc
     6301 atgctcaacg catttcggta catgggtatg accgtggaga agtatatctc cctttttcat
     6361 cggtggcact ttgctctcgt tataaacatg gccgtgagtg agatatatca ttttttcgcc
     6421 gagatcaatc aaagcataat cggcgagaac cggaaaatcc aataccatct gatcaacctc
     6481 cgcctcgcaa tttcctctga cgcagagaat atcgtcctta atttcgttga gcattgcaat
     6541 aacctgtttg ggcgcatact cttcgggcaa atcgtttctc ggaccgtgat aaagcacatc
     6601 gccgagaagg ataagcctgt cggccttttc ctctttatat ttttcaataa gcttgcggca
     6661 ccagtatgca ctgccgtgaa tatcggaagc aatcaaccat ttcatgcttt atgtctcctc
     6721 tccgaatgtt cttctttttg atttaaccag ttcctcaata aacatgttgt cgatttcgga
     6781 aagagtatag tctctgcgat ggataagcac atctttatac actcgcttat tatcaacaca
     6841 gtatttttga acgagaccgt agcggtcaag caaggtttgc ggaatcggcg atgaccacat
     6901 aaaggtttcg tgattttcgg caagcagctc aaactgactt gcacgttcaa aaacaaaaat
     6961 ctttctatcc gaattatccg gaagctcctc cttcttgacc tccgaaaacg gaagcgaagg
     7021 cacatacgga tcggcatggg caatcataac aagattgcgg agatcatcat aagtaacctc
     7081 ctcctttttt gcaagaggac tgtccttatg ataaagaagc ttgtagcgga actcggttat
     7141 aagctcatat tccaatccct tttcgtccat catgttttta tagtatttgt caaagctttc
     7201 ggcatagcgg actattccga gcttaaactc atcgttaagc aaattcttta ttgcgcgcaa
     7261 agcattggtt tccttataaa aaagctctgc ttcggtatct tttccaatca gctttgaaaa
     7321 ctttgcaaac gcatcggaaa tatagcttgc cctcggaaca gaaatcgaga actgagttct
     7381 tgcggcatgg tcatcgctga acatattttc aattgcatca acctgtttca gcaccgtttt
     7441 ggcatagcga acaaagacct cgccgtctgg ggtgggaaac attccttttg cgcttctttc
     7501 aaaaagcgtt accccgagac ttgtctccaa ttcttttatg gcacggctta gtgccgactg
     7561 acctacaaaa agcttttcgg cagccttgtt tatggatttt gtttctgcta tttcaactgc
     7621 atatttcatg tgtaacagat tcatgtctcg acctccgttt gcgctgcaat atttataggc
     7681 aacaccgcac aaattgaaac atagcgccct gtttaaatct tgctgcgcaa ttcatacacc
     7741 tcaaaagtgt ggcattgcct atagtatatc ataaaaagtg tttttttcaa tagatattgc
     7801 gacgaagcaa taaaattcaa agcagatact tttgacaaaa tgtcataaat gatataagtc
     7861 ttgaaaaatc aaaattcata taatataata aaagccaaag ggtagcattg tcgattgaag
     7921 tttacgtcct cattgtaagg gtaaagagtg cagccgcctg ccgcattgcc ggtgccgcaa
     7981 aagcattata aagccattaa aaggcttgtc ggtattcggc ttttttcgac agaaataaaa
     8041 atttctctaa attatttaaa acatattgca ttttttatta ttttatagta taatatgctt
     8101 tgtcgaacat ttgtacggat acggagtgtg attataaatg gctgataaac aaaaagctct
     8161 tgaaacagct atcgctcagc ttgaaaaaca atacggaaaa ggaacagtca tgcgtttggg
     8221 acagaacgtc gccatgaacg ttgaggctat ctcgacggga tcaatggctc ttgatgtcgc
     8281 aacaggtatc ggcggacttc caaagggcag aattgttgaa atatacggcc ccgaatcatc
     8341 cggtaaaaca acgcttgccc tgcacgcggt tgccgaggct cagaaagccg gcggagaggc
     8401 agccttcatt gatgccgagc atgcccttga ccctgtttac gccgcaaacc tcggcgtgga
     8461 cgttgattca ttgcttgttt ctcagccgga taacggtgag caggcacttg aaatcgccga
     8521 agctcttgtg cgctcaggcg ctattgatat tgtggttgtc gactctgttg cagccctggt
     8581 tccccgttcg gaaattgagg gtgaaatggg tgattcgcat gtcggtcagc acgcaagact
     8641 tatgtcgcag gctctcagaa agcttgcagg cgcaattaac aagtcgaata ctattattat
     8701 atttataaat cagctccgtg aaaaaatcgg cgttatgtat ggcaaccccg aaacaacaac
     8761 gggcggccgt gcgctgaaat tctatgcttc aatgcgtatg gacgtcagaa aagccgaaac
     8821 aatcaaaggt gctaacggcg aatttgtcgg ctcaagaaca agagttaaaa ttgtcaagaa
     8881 caaggtcgct cccccgttca aggaggctga atttgacatc atatacggcg aaggtatctc
     8941 taaggtcggc gagcttcttg atatcgcagt tgcaatggat attatccaca ag
//
LOCUS       DAWBSW010000073         8961 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1597_length_8961_cov_2.515046, whole genome shotgun sequence.
ACCESSION   DAWBSW010000073 DAWBSW010000000
VERSION     DAWBSW010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_8961_cov_2.515046"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    626..641
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    947..956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5074..5083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2480 a         1880 c         2197 g         2368 t
ORIGIN      
        1 gaataggatt atggaaaatt ccggcgagcc gataagtccg tacaaagaaa aaaacgcaga
       61 cttcttcttc gataaagaaa aagacgcctg cgacaggata tataaattta ttgcggaatg
      121 acatgtgatg ttgatttcga ttgtaaaaaa ccaccggctt gtgaccggtg gcttttattt
      181 aaccgttctg ttttgcatat gcctcggcga ccgccctggc gagctgatcc ggacaggatg
      241 tattcttgaa gccgcaggtg attccgctca gcttcttttc gatatccccg accttcattc
      301 cgtcaactat tgcggaaatt cccttgaggt ttccgttaca gcctcccgtg aattttacgt
      361 tccttacaac gtcgccgtca aggtcaaatg tgatgcggga tgagcatgtg ccctttgttt
      421 tgtattcata tgtcattgct ttgtacctcc gaattattgc tgaaaatatg ataattcaat
      481 ttctgctttt tgtcaaggtc aaagcattat atacatcagc gccatgagca aaagcatgtc
      541 tccgccgtca cccgaaagca ggagcatcat tgcgattata agcttcatat cgccgtcgaa
      601 aagatttccg agcggattgt taaatnnnnn nnnnnnnnnn ncggcggagg gggtggtgag
      661 ggctgcttgc ggaaaacagg ctcaccgttc ggcggcggag ttgcacgcct gtacatctcc
      721 tgagccctca ttatcgcctg acgctcggcg gcgttaaaat cctgttgcgg catatctatt
      781 catcccgatc tgtcaagtta ttgattagcg gtaaaatttc aagcagttta agtatttgaa
      841 ttgcctggtc ggctcttttt tgtctctcgg ggctgagatt cggcttcagt gcctctatga
      901 gcttgcagcg tttgcccgac tcgccgttca tcgctcccat tattttnnnn nnnnnncttg
      961 cctaccattt tcgggtcgat tgaggaaaac atatcggagg cggacggctg cgactgtttt
     1021 tcttttgctt catttccctt gccgaaaacc gcttcggcag tggctttcag tccttctatg
     1081 tcgtcgggag tgagagacga tagaatttca ttgatgtcca tttttcttgc cgttgagctt
     1141 ttccatgatt ttttgagcgg caggggagga aagcaccgca tttattttat ccctgtcgcc
     1201 gagaatctcc tgtagctcgt ctttttcatc tgcattcagt ccgctaatca gtttgcttgc
     1261 ctttttttcg gcgtctgcct tatcggtcgg tgcagagaaa aaattcggaa aattattatt
     1321 tgccatttac actcactctc caatgattta taatgattat ataatttata ttatgaaagg
     1381 tgttgtatgt atgcgcctgc tcgttttgtc cgattcccac ggcaggaaaa atcttttact
     1441 ccaagtcgtt gagctgcacc cggaagcgga tgccgtgatc ttcctcggcg acggtgaaag
     1501 agatattgat tttctcaaag agcttcatcc cgaaataaag ctttatgccg tctgcggcaa
     1561 ttgcgatttc aactcgtctt tgccgccttt tctgatagaa aaattcggtg ggaggacagt
     1621 ctatgccgtt cacggacatt atgaaagcgt caagtattcc cttgccgttt tgatgcaaaa
     1681 ggcgcataac tgttccgctt ctatagcatt atacggtcat acgcatgtcc ctgatacaaa
     1741 atatgaggac ggaatcctgt atgtcaatcc cggctcgatt tctgtcggca aatatgcaat
     1801 ggtcgatata acggacaagg ggatcatgcc gattctctgc gatttgtata aatgaagtaa
     1861 taaacctccg caaatcagca tagattaaat tagctgataa acggaggtta attatatgtt
     1921 tgtattttct ttcaaggcga acaagcctaa gctgattatt gctctttgcg ttgttatttg
     1981 tgtactgatt gccggtacac ttgctctcag cggcagaaaa aagccggttg tcaatgataa
     2041 ggtgatcaat tacaaggcaa aaacagcctc ggaatgtatc acgtttttgt ctcagttcgg
     2101 ctggactgta aacgaggaac ccgtggaagt cagagaggtg attattccgt ccgagtttaa
     2161 cgatacatat gaagcataca acgtcattca aaaaaatcag ggacttgacc ttgaaccgta
     2221 taaaggattc agagcaaaaa aatggatttt tgaggtaaaa aactaccctg aatatcctgc
     2281 cgacagcgga tatatcaggg caactttact ggtttattac ggagccgtaa tcggtggcga
     2341 tatttcgtcg attgagcaaa acggctttat tcagggcttt gagtttccga atgtttcttc
     2401 gcgggaagct gggcaatgat tatcgccgcg aacatgaccg cacagccgat atactcaaca
     2461 accgtcatac gctctccgag aatgatgaaa gagaatatga ccgagaaaac ggattcggtg
     2521 cagagaagca tactcgcaag cgtcggttcg gtatatttct gaccgatgat ttgaaaggta
     2581 aaagcacaac tgcagctcat tataccggcg tagagaatcg gaacagcggc ttgcataatt
     2641 ccgtccatgc tcggcttttc aaagatgaac atgagaatgc aggaaacaat gcctgttacc
     2701 atatactgac cgcatgaaag aacgatggcg tcaatcttcg aagcatattt gtcgataacg
     2761 attatatgca aggcgaaagc gatcgagcaa agcaatgcaa gaatttcacc cttgccgagc
     2821 ccgaacgagg atgagccgcc catgcaaatc atatacagtc cgccgacacc gaggaaaact
     2881 ccgagccatg tcgtgacggg ctgtttcttt ttatatatta taaaagaaat cagcggcaca
     2941 agcagcatgt ataaagctgt gataaagcca accttgcccg gctcgatata gtcaaaggca
     3001 aactgctgga ggtttgagcc gatgcaaagc attgtgccga cgattataat tccttttatt
     3061 acgtcttttc ggttcgattt cttttcggca ggggagagct tgacgatgtc tttatttttt
     3121 atgcttctga aaatgatgat cggcagaagc actattccgc ccaaaaggct tcttattccg
     3181 ttaaatgtga agccgtcaac atattccatg ccctgtttct gtgcaacgaa tgagagaccc
     3241 catatcattg cggcgctgat tagcagaatc ggaccgatta ttctcttctt gtttgccatt
     3301 ggataggatt cctttcaatt tgaactgccg cgtgagactg aactcacaaa tatacatttt
     3361 atcagccgag tagggatatg tcaaataaat atttgatttt ttttcttaat gtggtataat
     3421 attttttgaa ttaaaatttg ttttgctacg gaggaatgaa aatggctgtt tgtaatgtta
     3481 tttcacatcg cggttcaaat aaaatcgcac cgcagaacac tcttcctgct ttcagaaaat
     3541 ccattgattt tcatgccgac ggctttgaaa cggacgttca tttaaccttt gacggcgtcc
     3601 cggttatctg ccataactat actatcgacg agacctcgaa cggaaagggt cttattgcaa
     3661 atcagacact tgattactta aaaactcttg atttcggctc gtatttccac agggcctaca
     3721 agggaacaag aattcccaca cttgaggaat ttcttcgcct ttgcgaaaaa gctaagctca
     3781 aggtgctcaa tattgaaatt aagccgccta agaacaagga ttattcgatt gtgccgaaaa
     3841 caataaatat ggtcaaggct cacggacttt tcaaggagct tcttatttca agctttgacc
     3901 caatcgcatt gacaatatgc aaggacgttg acgagaactg taagacaggc tttctttaca
     3961 gtccaaacag tccgaacttt ttcagaattt acggcaaaga ggttgatttt gccaaatgta
     4021 tcggcgcaga cgctttgcac ccgtatcttt ggcttgtcga taaaatcctg gttgacaatg
     4081 cccataaaaa cggcatgacg gttaatccct ggactgtcaa caaggagaga gacataaaaa
     4141 gactcgttga attgggtgtt gacggtgtta ttaccgatgt tccgaatgtt gccaaaaaga
     4201 ttattgaaga attataataa acaataatta ttatagctta taagcgactg ttaaaatacg
     4261 gtcgcttttg cttttatttt ttaataaaac gttgttgata attatcttta aatatgttaa
     4321 aattataaaa attcagctat aaaatggggg ttgtgcaaat gaattgctgc tgcaaaaaaa
     4381 gcgatttgtc gcttattaaa cccgtgcttg acgagtacgg cgatattaaa ggcagcctta
     4441 taaccgttct tcagcaggct caggatatct acggctatct tccgaccgat gtgctctatt
     4501 gtattgccga gagaataggt gtttctcctg ctaaggttat gggtgttgca acgttctatt
     4561 cacagttcag actcaagccc gtaggcaaat atcttatcat gctctgccag ggtacggcgt
     4621 gccatgtcaa cggttctcag cgaatcgagg cagccataac tcaggagctg ggaattgccg
     4681 acggcgaaac gaccgacgac ggacttttta ctcttaaaaa cgttgcttgt ctcggctgct
     4741 gcagtctttc tcccgttatg atgataaacg gcgaaaccta cggaagtctc actccgtcaa
     4801 agacggttga gatcctccgc aagcttaaag aggagggctg aaaatgaaga ttgttgttgg
     4861 cgagggaagc tgcggaatag ccgcaggcgc cgcaaaggtt tattccgcaa ttgagaatct
     4921 tatcggtttg acaggctcaa cgctgaccgt taccggttgt ataggaatgt gcttccttga
     4981 accgattgtc gatatttatg acggcggcga gcttaaaaag cgtcttgtca aggttcgacc
     5041 cgaggatgcc gaaaaaatta tcgcatatgt aaannnnnnn nnntatgtaa acggtgacga
     5101 aaacgccgtt aacgatattg aaatttccga tgacgataag ctttttcttg aaaagcagac
     5161 aagaatcgcg cttcgtcact gcggaattat aaatccggaa attatagacg aatacacagc
     5221 ctgtgacggc tatagagcac tcgaaaaggt gctcaaaaca atgactcccg aggatgttat
     5281 cgaagaaatc aagatatcgg gactcgcagg tcgcggcggt gcaggcttcc cgacgtggtt
     5341 caaatggaac gccgcaaggc agtcgccggg cgagacaaag taccttattt gtaatgccga
     5401 cgagggtgat ccgggcgcat tcatggacag agccgttatt gagagcgatc ctcataatct
     5461 gattgagggt atgctcattg gcgcatatgc tatcggcgca aaggaagccg ttgtttatgt
     5521 ccgtgccgaa tatccgcttg cgattgagcg attgaaagaa gcaattaaac aggccgaaga
     5581 aaagggtttc ctcggtgaaa atattatggg aacgggattt tcatgcaaaa tgagaatcaa
     5641 ggcaggcgca ggcgcattcg tttgcggcga agaaacggct cttattgaat ctctccaggg
     5701 cgagagaggt atgccgaggc tcaaaccgcc gttcccggct cagtcgggct actggtataa
     5761 accgtctaat atcaacaatg ttgagacctt tgccaatgtt tcgtggatca ttctcaacgg
     5821 cggcgagtcc tttgccgcaa tgggaacaga tggcagcaag ggcacaaagg tatttgctct
     5881 cacgggcaaa atcaaaaggg gcggacttgt tgaaattccg atgggcaaaa cgctcagaga
     5941 tgttattttt gacatcggag gcggaatcaa gaacggtaaa aaattcaagg ccgttcagat
     6001 gggcggcccc tccggcggct gtatacctgc cgaattgctt gacacggtaa tcgactataa
     6061 ggcgctcggc gctacgggcg cgatcatggg ttcgggcgga atggttgtaa tggacgagac
     6121 gacctgtatg gtaagcatgg cgcagttctt ccttgatttc actgccaagg aatcctgcgg
     6181 caagtgtatt cattgccgaa tcggaacaaa gagaatgttg gaaattctca acagaatagt
     6241 caagggcgag ggcagagaag gcgatatcga gcttcttgag gagctttgct attcaatcaa
     6301 ggacggtgct ctttgcggcc tcggtcagac tgctccgaac cccgttctga caacaatcaa
     6361 gtatttcaga aatgagtatg aggctcatat aaatgataag aagtgccctg caaagagctg
     6421 tcccgacctt ttggaatata cgattgacgc cgacaagtgc cgaggctgca cgctctgtgc
     6481 aaagaaatgc cctgccaatg cgatttcggg cgaggtaaaa tcggcacaca agatagatcg
     6541 ggacaagtgc atcaagtgcg gctcgtgcgt aagcgtatgc cgattcggtg cggttaaggt
     6601 agattgagag gtgatttgaa tggataaaat taaggttacg atagacggca aagagctgac
     6661 cgccgaaaag ggtcagacca tccttcaaat tgcccgtgat aataatatcg agattccgac
     6721 gctttgttat attgacgagg taaaggcata cggtgcgtgc ggactttgcg ctgttgaagc
     6781 cgagaatata cctaagcttc tcagagcttg tgcaagcgtt gcatcggacg gaatggttat
     6841 caatacaaat actccgagag tgcgccggtc gagaaaaatt gcccttgaat tgcttgtttc
     6901 cgaccatacg ggcgactgta aaggcccttg ctctctcaac tgtcctgccg gaacggactg
     6961 tcagggctat gtcaagctta tctcagaggg caaatataaa gaagccgttg agcttgtcaa
     7021 ggaaagagtt cctctcccgg cttcgatcgg acgggtttgt ccgcatccgt gtgagactgc
     7081 atgccgcaga aagcttgtcg aggagccgat ttcaatcgct tttctcaaga gctttgccgc
     7141 cgatgaggat ttgaaatcag gcaataagtt tatgccggaa gtgaagaaag caagcggaaa
     7201 aacagtcggc ataatcggcg gaggtcccgc aggactgacg gcggcatact tccttgccgt
     7261 caagggtcac aaggttacgg tttacgatgc aatgccgaag atgggcggta tgctcagata
     7321 cggaattccg cagtacagac ttccgaaaga ggttcttgac gccgagatag gctcgattga
     7381 ggagcttggt gtcgagatga aaaataatgt taaaatcggt aatgacatta cgcttgacga
     7441 gataaggaaa agccacgatg cggttattgc tgcaatcggc gcatggacga gcagctcaat
     7501 gggcgttgag ggcgaaggac ttgacggtgt gctcggcgga attgatttcc tacgttctgt
     7561 tgcgatgaaa aatcccgttg acatcggcaa aaaagtagct gttatcggcg gcggaaatac
     7621 cgctatggac gcctgccgct cggctgtaag gctcggtgcg gaagaggttt atattatcta
     7681 cagaagaacg agagccgaaa tgccggcaga aaaggttgag attgacgagg cggaggaaga
     7741 gggagtaatc tacaaattcc tatgcaatcc gtctgaaatc ataggtgaaa acggcagagt
     7801 gaaggctgtt aagcttcaga aaatgatgct tggcgagccc gatgcaggcg gacggcgcag
     7861 acccgttccc gttgagggcg agtttgaaac gatcgagctt gacactgtta ttatggcaat
     7921 cggtcaaaag ataaatatca aaggctttga acagcttgaa ttgacgaaca ggggcaacat
     7981 tgccgccgac gaaaagtcat tcagaacctc tcttgacggt gtttttgccg tcggtgacgc
     8041 aacgaacagg ggcgcctcaa tcgcaattga agcgataggc gaagcgcaga aggcatccga
     8101 ggtcgttgac aggtatctcc atggcgaaaa catcagctat aaaaagccgt tcgttgttga
     8161 aaagaccgtc acggcagccg atcttgccga cagagaaaag tgcgcaagag cgagaatgcc
     8221 gcagcgctct cccgaggaga gaaagcatga ttttgacgag ataaatctcg gctttactcc
     8281 cgagcaggca atgaatgaag caaagcgctg tttacagtgc ggttgtcacg attatcacga
     8341 ttgcaagctg ataagatatg caaatcaata tgatattcac cccgaacgtt ttgcaggcga
     8401 taagcataac tgccccaagg agcagaagct tgtttgcatt gaaagagatc agaacaagtg
     8461 tattctttgc aatctttgcg ttcgtgtctg tgatgaggag gtcggaaagg gcgttctcgg
     8521 tcttgtggga cgaggattta caacagtcat caagccggaa tttgattcgg cggagactgt
     8581 tgacttctgc aaaaactgcc tcaagtgcgt cgatgcttgt ccgacgggtg cgttaaaatc
     8641 attgtaaaag tgttgtatca ctgaattttt atacaatttt caaccggaaa gctttaaaaa
     8701 gttttccggt tgtcaatatt attacggcaa taaaaaacga gctgaaacga gcaaaacaga
     8761 gcaaaacgga gcaaaaaaga gtatttcaaa aaccattcaa aaaaactgat gatttaatcc
     8821 taataatttg caaaaaaatg ttgacatttg cgagtttctg cactatatta tatatcaata
     8881 tcgaaaggaa aaatttattt gcatatttcc gactgatatt tataccgtat tcggtaaaga
     8941 gaggggctgc ataattggaa a
//
LOCUS       DAWBSW010000074         8849 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1622_length_8849_cov_2.469638, whole genome shotgun sequence.
ACCESSION   DAWBSW010000074 DAWBSW010000000
VERSION     DAWBSW010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_8849_cov_2.469638"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6319..6328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2497 a         1900 c         1982 g         2460 t
ORIGIN      
        1 gcataagacc caaacagatt attgcgatta tcggccacaa ccgtgtgacc gcttcataat
       61 caaaatatga tataacaagg gaaacgcata tacccatcat aacagcgaga aacatcgttt
      121 tcgcatctct tgaaatgatc tgatcctcgg tcaggtttac tctcgttgcg ctgtaaacca
      181 tgactgttcc gactgcggaa agaatcatgc aaagacctaa aagaatcttg tctgttccct
      241 tgataaagtc ctttaaccaa tatacaattt ttcccatttt ttcacctccc gaggatactc
      301 atattattat atatgttatc gccgagtttt tcaagttttt atttgacatt ttatatttaa
      361 gtgttataat taaataaaat aaatcgaata ttgcgaaacg ccctgacgct gaatttttta
      421 aggcgaaaga accgttttgt gcgattttgc cgtattcttc attggatgcg gttttatttt
      481 gaaaaaattc tgaaagaaag taaatctgac ttaaaggaaa ggaaatcatt atgacagttt
      541 ataacccgaa atcacttaaa gccgaggaat tcatcaacca tgaggaaatt ctcgaaacaa
      601 tcgaatatgc agaaaaaaat aagcacaatg ttgagcttat agattcattg cttgaaaagg
      661 ctcgcccgaa gaagaacgac cgcggcgtta cctgtgcagg tctaacccac agagaggctt
      721 ccgtcctcct tgcctgcgac attcccgaga aggttgagga gatgtataag ctcgcaaatg
      781 agattaagct tgctttctac ggcaacagaa tcgttatgtt tgctccgctt tatctttcca
      841 actattgcgt aaacggctgt gtttactgcc cgtatcatta caaaaacaag catatcgcaa
      901 gaaagaagct ctctcaggag gaagttaaga acgaggttat tgccctccag gatatgggtc
      961 ataagcgact tgcaattgaa gcgggcgagg atcctgttaa taatccgatt gaatatattc
     1021 tcgaatgtat taagacgatt tattcggttc atcacaagaa cggagatatc cgccgtgtca
     1081 acgtaaatat cgccgcaaca acggttgaga attacagaaa gctcaaggaa gcgggtatcg
     1141 gcacttatat tctcttccag gagacctaca ataaaaagag ttatctcgag cttcacccga
     1201 agggaccgaa gcacgattac gattaccata cggaggctat ggacagagcc atggagggcg
     1261 gcattgatga tgtcggtctc ggcgttctgt tcggtcttga gggctataaa tatgaatttg
     1321 ccggacttct catgcacgcc gagcatcttg aagcggttca cggcgtaggc cctcacacga
     1381 taagtgtccc gagagttaag catgcggacg atatcgaccc cgatgttttc gataacagtc
     1441 ttgcggacga tatgtttgca aagatcattg catgtatccg tatcgccgtt ccttacaccg
     1501 gaatgattat ttccacccgt gagagcgaag ctgtcagagg aaaggtgctt gattacggta
     1561 tttcgcagat cagcggcgca tcgagaacct ccgtcggcgg ttattgcgag gaggagcgtc
     1621 ctcacgattc cgagcagttc gacgtttccg accagagaac cctcgatgag gttgttcgct
     1681 ggcttatgga aaaggatcat attccttcat tctgcacggc ttgctaccgt gagggaagaa
     1741 caggcgacag gtttatgagc ctttgcaaga gcggacagat tctcaactgc tgtcacccga
     1801 atgcattgat gacgcttacg gaatatctca ttgactacgc ctcggaggaa acgagaaaga
     1861 taggctttga aatgatcgac cgtgagcttg aaaaaattcc aaaagaaaag gtcagagaga
     1921 tcgcacgtca gaatattgag gctataaagt cctcgaacag acgagatttc cgcttctgaa
     1981 tgtaaccgat ggtcgattaa aagcacaaac cttatcggta tattttccgt catatcacat
     2041 aagccatctt gataatacga cagtataacc tgcgatgcct tatgcaatct gacaaaaaat
     2101 cttctcgcta atctttgcac tttaaatcaa tcatcggtta ctaaatatca tcttcggata
     2161 tttacatcac gaaaaaatgt gttataatat ctcggaggtg atatttttgt gtgtttatta
     2221 cagccataat gttgaggaaa cttacagaat cggagagagg ctcggctcga aattaagaag
     2281 cggagatgtc gttgcttatt tcggcggaat gggtatgggc aaaacgacat ttactcacgg
     2341 tcttgctacc ggtctcggca taaacgaaaa ggatgtcagc agtccgactt ttgcccttgt
     2401 ccatgaatac agaggcaagg aaaacacgct ttatcacttc gatatgtatc gaatcgaaag
     2461 ctgggacgac ctttactcaa caggcttttt tgattatctc gactacggcg gtatacttgc
     2521 cgttgaatgg agcgagaata tcgaaaacgc actgcccgat aacagcataa gggtcacatt
     2581 caagcccggc aaaaatgaaa acgaaagaat aatccaaata gagggagttg atcttacatg
     2641 aaaatactcg gaatcgacag ctcggcaaga tcggcttccg cagcaataac ggacggtgaa
     2701 aaggttctcg gctgctttta tacaaacacg gggctgaccc attcgcagac gcttatgccg
     2761 atgattgaaa gccttttgaa aaatgcaaac gtcaggcttg acgatattga tcttatagcc
     2821 gtcaacaagg gccccggctc attcacggga ataagaatag gagttgcggc ggcaaagggt
     2881 atggcggatg ttcgaaaact ccctgtttac ggtgcttcga cgctttgctc aatggcatat
     2941 aatctcattg attcggactg tatcgcttgc tgtgccatgg acgcacgttg cggtcaggat
     3001 tattatgctt tgtttgatat tacggacggt aaaatcacaa gattgaccga ggataacgcc
     3061 gattcgatag aaaatgttga aaaaaagctg aaagaataca aaaaaattaa atttatagtt
     3121 ggcgacggcg cagaaatatg ttataataat ctgaaaaaca ttgatgatgt gcgccttgca
     3181 tcgcaaacac accgctttca gtcggcaatc ggcgtatgct ttgaggcgat gaatgcgccc
     3241 gaggatcaat atatcgagag tgcaatgctt gtgcctgagt atctcagact tccgcaggcg
     3301 gagcgtgaac taaataaaaa acttagaaaa ggcgagtgat ttaatgaaaa tagcaatcgg
     3361 agccgatcac ggcggatatg agctcaagga gtccatcaag aaacaccttg aagaagtcgg
     3421 cggatatgaa atcactgatt acggtactca cagcaccgat tcggtcgact atgccgagat
     3481 agcattcaag gtaggtaacg cagtaacgaa cagtgacgag aaaattctcg gaattctttg
     3541 ctgcggcacg ggtatcggca tcagcatggc tgccaacaaa gtcaagggcg ttcgtgcggc
     3601 ttgctgttct gattatttca gcgctaaatt tacgagactt cacaatgacg ccaacattct
     3661 ttgtatgggc ggcagagttg tgggtccggg acttgccaat gagatggtcg atgttttcct
     3721 caacacggag tttgagggcg gacgtcatca gagacgtgtt gaccagatca cggctattga
     3781 aaacggtaca ttttaattta cggaggtata aatatgtcaa cagtaaccat taccaatcat
     3841 cctcttatcc aacataagct ctctatcctc agagacaaga acacttcttc caaggatttc
     3901 agatcacttg taagcgagat tgcaactctt gagtgctacg aggcaacaag agaccttccc
     3961 gttcaggaag ttgagattga aacacctatc tgcaagtgta tcgctcataa ccttgagggc
     4021 aagaagttag cgttcgttcc gatcctcaga gcaggacttg gcatggttga cggcgttctt
     4081 tcgctcgttc cgtctgcccg tgtaggtcat atcggtcttt accgtgaccc cgagacactc
     4141 aagcctgttg aatattactg taagctcccc gtagatatcg cagagcgtga cgtaattgtt
     4201 cttgacccga tgctcgcaac cggcggttcg gcaattgacg ctatttcact tatcaaggag
     4261 agaaatccga agtcaattaa gttcatgggt attatcgcag ctccggaggg acttgcggct
     4321 ctccagaagg ctcatcccga tgttgacatt tactgtgcag ctctcgacga gaagctcaat
     4381 gagcattgct acattgttcc cggtctcggc gacgcaggcg acagaatctt cggcacaaaa
     4441 taagctaaat aatactacag aggctcagga ttttttcccg agcctctttt ttttgatttg
     4501 taatttgatt ttcggcagaa atttctctta gctttcctgc acggagcccg gaaagctgtc
     4561 tgcctcgcaa gcatatttac cgtaactcct cagtgcctca tcgaaccaag gtcagataaa
     4621 aataaggcgg aatccgcagt taatataatt gctgaaaaag gacgacaatc tgccgtcctt
     4681 tttgaatatt tattacttca cataagccgc gacatcgcgt tcatagtgtc cggtattttg
     4741 gaagccctcg tcagttctga gacatgagcc ccattttctc gcgatttctt cgtaggaatc
     4801 cgtttgaacc tttgcccgct caaagccgtc ctttgcaccg aacattaccc agtcggagtc
     4861 ccacattccg tgacccgaag ccttgtaggt ccaaagcatc cagttgtagt tgagattttt
     4921 catcgctttg aagcagcttt cccatgttgt tgtaccaagg ggataaaatt ctcccatcat
     4981 cagcggcgtg ttcatgtaat agagcttagt cagcgttaca aaaagaacga agataaaatc
     5041 ggatttctga tagaaatgaa cctgataaac aacattcttg aagcctgcaa gagccttgtg
     5101 gggcagtgca aacgccgtcc agatacattc gagagtaatt atatgatcct cgtcaacggc
     5161 ccttacggcc ttgtatagac gcttgtattc ttttgtgttg ttaattcttc ttgttacctc
     5221 gccgtaatcg caatcgcagg acggctcatt aagaagatcg tacattgcta cagccggatt
     5281 acccttgaag cgtgaagcaa ttgctgtcca aagctcgtcg gcaagcgttc tgaaaaattc
     5341 gccctgctcc gagttctcat aaagccggca tgagtctctc ttgccgctgt ggggagcgtc
     5401 actctgaaaa ccgggtgcac cgtgcatatc gagtataacg taaatctgtc tttttgagca
     5461 ctcctcgact atccagtcaa gataacgaaa atcccattcg ccgtttttgt cgagtatttt
     5521 tgtaccgtta tcgtcggaat agaaatttct gtaccagaag gggacacgga cgcagttgaa
     5581 gcccatttcc ttgatttggt caaggtcata ttccgtgatc cagttgtcct gatatgtatt
     5641 catcagcgca tatgctctct ctgtgccgaa gcggtcaatg agagtttcaa gcatatcgtg
     5701 atgatcctgc gcttcttcat agggagaaag ccaatcctct tgaatgagcc atgaaccgag
     5761 gttcacgccg cgaagaataa tttcctcacc tttttgattg tagattttat tgccctttgt
     5821 tacgagaaaa tcctcctgag tgaagccggt gtcaattccc gccgccgcag cgctgagcgc
     5881 aaagggtaga atgagcacaa aacacattac aacgcataaa aatcttttga ttgtcttttt
     5941 catagaacca acctccttta attgcattat atcattaaaa aattattttg tctatacaga
     6001 taaagaaact cccgaaatcg agatgataac gggagtaaaa ttattatttt ttcttttcgg
     6061 ctgtgccctc ggaatttctg aaaacaacaa atttatcaaa gatgaactcg gtaatcatgt
     6121 ttgcaagcat ggttatgata agcactatat aatcgacggc ttccgaattt gaatatttgt
     6181 ttgtaaaatg aacaccgata acagtagaaa tcggcgtaaa caccgcataa tatgcaaaga
     6241 ccttgagcat tgcaatcgga acattgttgg ctgatttgaa tgtaaacttt ctgttgaacg
     6301 tgaagttcca tataacggnn nnnnnnnnag atacatcgga ccgtattcgt tgttcattat
     6361 tcttgcgaag gtctcatgct cgagcatcag attctgaaag aaatcaagct taatgatgac
     6421 ctctttcatc aatgtaaagc tgccgaactg aatgattccc gcggaagcgg agaacagaac
     6481 gaactttata aattgtaata tgtctttttt gcttttcttt tcgctcataa aatcacctga
     6541 ttcacttttc ttactataat acatctcttt ggcgatttat gcaagaattt ttttgactca
     6601 ttgcaaaaaa acatccctgc taaaaacaga gatgtttcta taatttattt aagtcccttt
     6661 ttatcaagca gagtgttgat cttttcggcg gggtcgagaa ggtcgctgat tgatgtgtca
     6721 tggttaaggg tgtcaatgat gtatgaaaga tactttgaca tatcaactct tatataccaa
     6781 tccttgctga gaagctcggg ggtaccgtaa atgaggttag ttgtgaacac acggtcgata
     6841 atgccttcct gaaatgcttt atcgaatgca tcaaagccgt tacagaaaag tccgaatgcc
     6901 gagaaaatat aaattctggc agccttgagc tccttgagct ttgttgcaac ctcgatcatt
     6961 gattcgcctg aggaaatcat atcgtcaacg atgataacat ccttgccctc aacgttgtcg
     7021 ccgaggaact catgagcgat gatcgggttt ctgccgtcaa cgattcttga gaaatcacgg
     7081 cgcttgtaga acattccgag ctcaacgccg agaacggtcg aatagtaaat acaacgtgac
     7141 attccgccct catcggggga gataatcatt atgttgtcct tgttgatctt aaggttgtca
     7201 acgttgttga ctgcggcctt gatcatctga taggcggggg atatgctctc aaatccgcaa
     7261 agggggattg cgttcattac tctcgggtcg tgtgcgtcga aggtgatgaa gttttcaaca
     7321 cccatgttgt gaagctcctg aagcatcatt gcacagtcga gcgattcacg gctcgaacgc
     7381 ttgtgctgtc tgccctcata gagcatcggc ataataacgg aaactctctt tgccttgccc
     7441 tcaattgcgc tgattgcacg cttgaggttt gcaaagtgct cgtccggtga catcggaacg
     7501 gtcattccgt acatcttgta tgttacgccg tagttaaaaa catcgcaaag aatgtagatg
     7561 tcatagcctc tgaccgtctg gttaagcgct gctttacctt cgcctgtacc gaaacggggg
     7621 aaatccatgt tgataagata ggttgatttt tcatatcctg cgaatgcaag cgtgtccttg
     7681 tgctcgctgt tacgctgaaa tctccatctg atcaggtaat tgtcgatctt gttggcaagc
     7741 tcttcgcagc ccgggagagc gatgatacca agctgtccga caggtatggt gttgtaatta
     7801 ggttcacttc tcatttatac tatccgcctt tcagagttat atggttattt tactataaaa
     7861 gagcaatttt ttaaagagat tgacaagctt tttgtcgaaa aatatatcac aaataaaatg
     7921 gcataatttg ttaagtttat acaaaactat gttctgcttt tatgacggtg aaataactgt
     7981 cgttcacttc ggaaattttg aggctgattt ctttgataat ttcattcggc acttccttga
     8041 agccgtaggg gataacaaca tcaaaaatga gatttgtgtg catggggccg tcaacaactc
     8101 tgaaatcatg tatgctgagc tccgggttaa tgttctttac gatattatct gtcatttctt
     8161 ttagctcgtt tacacgctca tcgttgacaa caatcgggtc atagtgaatc gtaagatcaa
     8221 tgcctatttc tttttttata tcgttttcga tgttgtcgat tatgtcgtga cctaccatga
     8281 cgtcaatatc ggcgggaatt tcaacatgta ctgttgcgaa ataatgctcg ggtccgtagt
     8341 tgtgaacgat gaggtcatga attccgctaa catggtcgta tgccaatatc ttatttttga
     8401 tttcgtcaac aagctccttt gacggcggct gaccgataag agagcttaga gtatccctga
     8461 atatatcaag acctgccttt aagacgaagc aggcaacgat aattccgagc cagccgtcga
     8521 tatggaaaga agggaagaat tttgaaagaa ttaaggcgat cagcgttacc gaggtcgcgg
     8581 caatatcact gagactgtcg gcaacgacgg cggtcatggc aggggagtcg attcgtttgc
     8641 cgaggttctt attgaaaaat gcaagccaaa gctttccgag aatcgaaacg gcaatgataa
     8701 cagaaccgat aagactgaac ttaacgtcct cgggcgtacg gatctttttt accgactgaa
     8761 tcgcaagctc aacacccatg aaaagaatga tgaacgaaac tatcatggca gtaatgtatt
     8821 cgattctgcc gtgaccgaac ggatgatcc
//
LOCUS       DAWBSW010000075         8803 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1634_length_8803_cov_2.753086, whole genome shotgun sequence.
ACCESSION   DAWBSW010000075 DAWBSW010000000
VERSION     DAWBSW010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_8803_cov_2.753086"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2494..2503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3295..3304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2286 a         2108 c         1891 g         2498 t
ORIGIN      
        1 agagctttgc acctgccgga atgtggtcat ctaccatcag aagatgaagc ttttcctcgc
       61 cctcttcctc atgaatagcg gaaagaagca taccacagga atcaatgccc atcatagctc
      121 tcggcggaag atttgtgatt gcgatacagg tcttgccgat aagctcctca ggctcataat
      181 aagcgtgaat accgctgagg atcgttctgt cctcgcctgt tccgtcgtca agtgtgaact
      241 gaagaagctt cttcgacttc ttaacggcta cgcagtcctt gatcttaact gctctgaaat
      301 ccgactttga aaatgtatca aagtcaacat attccttgaa cagcggctcg atctcgacct
      361 ttgagaagtc gattacttcg ggagcagcct tttcttccgt tgcttcctct gcaaccgtct
      421 gagccggctt gtcgtacttc ttgtccgaat caagcggttt cattgtcggg aagagcaaca
      481 catctctgat cgaggggctg tttgtaagaa gcataacaag acggtcgata ccgtagccga
      541 taccgcctgt cgggggcata ccgtactcca tagcggtgag gaaatcctca tcggtgtggt
      601 tagcttcctc gtcacctgca gccgcggcag cgtcctgagc cttgaaccgc tcacgctggt
      661 cgatcgggtc attaagctcg gaatatgcgt tacacatctc ccaagtgttt atataaagct
      721 cgaaacgctc aacctgatcc ggtcttgacg gctttctctt tgtaagcgga gaaatgtcaa
      781 ccgggtgatc cataatgaat gtcggctgaa tgagcttgtc ctcgcagaac tcctcaaaga
      841 agagattgag aatgtcgccc tttgtgtgac gctcctcgaa ctcaatatta tgctctttag
      901 caagcttctt tgcttctgca tcgtccttga cagcatcaaa atcaacgccg gcatacttct
      961 tgactgcgtc agtcattgta agtctctcga acggcttgcc gaggtcgatt tccttatcgc
     1021 cgtaggtgat ggtcgttgaa ccgcaaacct cggttgcaag gtatctgaac atgctctcgg
     1081 tgagatccat cattccgtga taatccgtat atgcctgata aagctccatg agagtgaact
     1141 cgggattatg tcttgtgtcc atgccctcgt tacggaaaac tcgaccgatc tcgtaaaccc
     1201 tctccatgcc gccgatgata aggcgcttga ggtaaagctc aagggaaatt ctgagcttga
     1261 agtcctcgtt gagtgaatta tgatgagtga cgaacggtct tgccgccgca ccgcctgcgt
     1321 ttgaaacaag aataggagtt tcaacctcaa caaagttctt ttcgtcaagg aagtttctta
     1381 tcttctttat aataagcgaa cgcttgtaga acgttgtctt aacgtcggga ttaacgataa
     1441 ggtcaagata acgctgacgg tatcttgtat cggtatcctt aaggccgtgg aacttctcgg
     1501 gaagaggaag aagcgacttt gaaagaagtc ttacgttctg agcatgaacg gagatctgac
     1561 ctctgcgtgt tctgaaaaca aagcccttga cctcgataat atcgccgata tcccattttt
     1621 tgaactcggc aaactcatcc tcgccgatat cgttcattct gacatatacc tgcatacgtc
     1681 ctgcgcggtc ggtgatatca ataaagttgg ccttgcccat gtctctccag gtcatgatac
     1741 gtccgcaaag agttaagtcc tcaattctga cctgctccgg tctctgatcc tcgggaagct
     1801 cgtttgtctc tgcctcaagc ttctcgaagg cggctgtaat tttcgcactg gttgccgact
     1861 gctccgcagt cgttatctga aacgggtcct tgccagcttg ctgaagcgca acaagcttgt
     1921 caaccctgat ttttctgagt tcgttggtgt tctcctctgt ctcaacaacg ggagtatttt
     1981 tttcttctgc catattcaac tctccattca tagcaaaaag ttttaacagt caaagcgggc
     2041 gtaaggctaa gcctgcgccc gatatgttta tattgaaatt tcaagaacct caaaagcgat
     2101 tacgtttccg ttcggaagat taacattgac aacgtcgccg accttattgc cgataagcgc
     2161 cttgccgact gcggactgat ccgaaataat accctcatcg ggatttgcct ggctctcgcc
     2221 gagaatcttg tattcctcaa tatcaccgta ttcaacatca cggatcttaa cctttgatcc
     2281 gacgtgaata gcctcggtag taattgcgct ttcgtcaaga attttagcgt tcttcatttc
     2341 attttcgagc tgagcgattt tagcctccat tttagcctga tcgttcaatg cctcatcata
     2401 ttcgctgttt tcagaaagat ctccgaatga tctggcctct ttgattcttt cggctatctc
     2461 gtcacgtccc gctgttttaa gatattccag ctcnnnnnnn nnntcatcat ttttcttttt
     2521 gtaagcctca gctgtaagca agatttcctg tgccataatt ttcatccttt caatgcaatt
     2581 actcggtctt ttgaccttga tacagataaa agcctgtacc tcaaagtcaa agtacagtaa
     2641 tatatctatt atagtgcatt cctcgaaaaa agtcaagtat ttgttaaaaa cgacttttaa
     2701 cgaagcgtta atataaagca acgcagcaca atcaaggcgt cgggatatgc aataaattat
     2761 ttcgtctgtt tgcgaaaaag ataactgaaa aaaccttcgc agcatgacgt aaaatcaagc
     2821 agtatatgcg cctcaattta tgcgctgtcg cctttatata atatgtccga aaaattcaaa
     2881 cattcctgat ttatcctttt tctatttttt gaacctcgtg agcgtcaagc aaacggcatt
     2941 cgccctcttt aaggctcacg tcaagcgtaa gcccgcccat ctgcgttcgc ttcaattcaa
     3001 ttacaccgtt gcctgcggcg gcaaacattc ttttgatctg atgatattta ccctcgcaga
     3061 tttttatttc aacaagcggc ttttcaccgt cggcaaggac agtcaccttt gccggcagac
     3121 attttgttcc gtcctttagc ccgataccgt tgcgaaccgc ttcaagctga ccctccgtca
     3181 cactttcggc aagccgtgcc tcataggttt tttcaatatg attcttcggg gaaaggattc
     3241 tgtgtgcaaa atcgccgtca tcggtaacaa gtacaaagcc ggtcgttgta atatnnnnnn
     3301 nnnntgttga gcatgctata aacaaagcgt tgaaaatcaa gctgttcgcc gttcacgcag
     3361 ataatatcct tttcgtcatc cgacttggct cccctgtcgc gaaccgttat tccgttgacc
     3421 ttgacagctc cgtttttaat aagctcctga acctctctgc ggctgaatct gccctgcgaa
     3481 gcgatcaatt tatcaagacg ttgaagcatt ttttactccc tgtcaagata atcaaggctg
     3541 acccagccct cattaccgtc ataatatgta tggccccagc cgtcggacac acggtcgatt
     3601 ttaagaaccg taccttcgct gagctcaagt atgatgtcgt tttgtgttcc gatatcggtt
     3661 ctgagcttga gtccgtccgt gttgactgta taatttcccg ctccctctgc tgttgttgtt
     3721 tcgtcggaag aggtcttttc agtcgttttc tcggtcggct tttcagtcgt tgtttccggt
     3781 ttggttgtcg ttgccgcttc agtccttgcg gaagcgtccg aagaatcacc gaacgaagca
     3841 agaccccatt tgccgtcatt gcaaatatat gctctgccgc ttacaacggg cgttgcccaa
     3901 tcataaatca tttgagtgac agccttgccc tcgcttgaat atattccgaa tttaccgtct
     3961 tttgtaacgg ttacatatcc gtcaaagcac tcatacggag tatcctcgcc gccgaaagcg
     4021 ctgtatccct taacggcatt gaatccgaac ggaagaaccg tttttccgtt tgaatcaata
     4081 tagccccaaa ctccgtttga ctcgaaagca gccattccgt tggagaagca gcctgcaccg
     4141 gaataaatca tacccataat gttcctgccg ttgatatata atccgtacgt gccgtcagaa
     4201 acatattttc cgtcaacgtc cttaacgccg cgtactgtct cgggcaagga ggcctttaaa
     4261 tcgtccttgt aaatttcgcc gttatcattc tgaacaaaaa cggcggaatt atccgttgtg
     4321 ctccaaagat attcatatct gagcgtatta tattctttgt aagccgtctc ggtggaaaat
     4381 gtcttgtaat caatataggt ctgcacggtc tcaccgtcgg aagacttgct aaagccgaga
     4441 aaatccttgc tgtttctgat agcaacaatg ctgtcatact taggctttat aaccgtcttg
     4501 ccgttataat caataattcc gtactttccg ttttgcttga ttttaaaaca gtcggcatag
     4561 cttatatcat agtgtcttgt gctgtcatta taatctgctc ttacaagcgg ttcgatcgct
     4621 tcatattcca tagtaggagc tatgatccat ttaagcgaaa caagagaatt gttattatcc
     4681 gagccaactt tgacaggttc tctttttgac gagcaagccg aaagcacgcc gagcatgctt
     4741 gcaacgcaca aaagtattga tatcagcttt atgaagcctg tgttcttcat cttttacacc
     4801 tctcggagaa gcatgacgac cttttgaaat aaaatcaata catactttct ctttttttct
     4861 tccttattgt cctgtaaata aaatagacaa caacgataac tgcgaaagta atcagtccga
     4921 tatatacgga catgacgtca ttaccttcta ttttaagatt atcccaaagc gaggtcatga
     4981 gcatgagcgt ctcggaatcg agattgtgga aatactcggt cgtcggagtg tgctcctcat
     5041 cgggatagag catttcgtta tctctgagcg aatattcctc attgtcaaga accgcccggt
     5101 taggagtagc ataacacaaa cactcggcat ttgcaagtgc aatgtcgcct ctgagcaaga
     5161 aattgatgta aagctcggcg gcgtccttgt tctgacagcc cttggggata cagatggaat
     5221 cgacgaaaat attcgtgccc tctttaggat aaacaaacgc aagatcagga ttgttctctg
     5281 ccatgccgat acagtcgcct gcgtaatatg cggctattgc agcctcgccg ccctccatct
     5341 tgttaaaaac gtcgtccata acatatgact gaacgacagg cttctgttcc ttgagctttt
     5401 gataagcctt tttccaatcc tgttcgcttg ttgtgttgac atcaattccc aaaaggaact
     5461 gtgcaatacc gaaagcgtcg cgggagttgt tgaacatgag cacctgaccc ttgtactttt
     5521 tatcccacat aatgctccag ctatcaggtg tgccctcaac cattttggtg ttatagacta
     5581 gtccgaccat gccgacattg tagggaacgg aatactcgtt cttcgggtca aagtaaagat
     5641 ctttgtactt cggaatgata tttttatagt tcgggatatt gtcaaagttt attttttcga
     5701 gcatgtcctc gtctatcatg cgctcgatca tgtagtccga cggaataatt atatcataat
     5761 ttgctccgcc gctcttgatc ttgttgtaca tattttcgtt ggaatcgtag gttgtgtaat
     5821 tgaccttgat tcctgtttct ttttcaaatt ccttgtttac gtcgagggta tcctctgcgc
     5881 cgtctgaaat atattcgccc cagttgtaaa cattgatttc cgttcccttg agcttggaaa
     5941 catcaatctg ctttgccgca acaccgaacg aggcggaaca gctcagggcg ataacgataa
     6001 cggcaaacaa aaccgaaaaa atctttttca tcattttgcc gcaccgcctt tcttctcctt
     6061 cctgttatct ccctgtgcct gaagaacatt gacaagaacg aggagaacaa ggataacgat
     6121 aaacataatg gttgaaagag cgtacatatc gggagtaaca cgcttcttcg tcatggagaa
     6181 aatccttata ggaagcgtct ggaactttgg tccgcttaca aaatagctga taataaagtc
     6241 atcaaacgaa agcgtaaatg ccataataag tcctgcgata acgccgggaa gaatcgacgg
     6301 aagaactacc ttgataaacg ctttaatggg aggacaaccg agatcctgcg ccgcctgaga
     6361 aaggtttgcg tccgtttgtc tgagcttggg caatacgttg agaatagcat agggcagact
     6421 gaacgttaca tgagctatga aaagcgtcca gaagccgaga actccgcttg cgccgagaat
     6481 cgagccgatg aatacaaaca gaagcatcat cgaaacaccc gtaacgattt cggggttcat
     6541 cataggaata ttagtaacgg tcataacgct tgatttaaca acgccttttt tcattttgtc
     6601 aattccgacc gcggcaagcg tgcctataac tgtcgctgta attgccgaag taacggcaag
     6661 aatgagcgta tttgtcaagg cggttatcgt tgcgctgtct ttaaaaagct caacatacca
     6721 ttttgttgag aatccctgta taacgcttgt actgttcgtt ccgttgaatg aaagcacaat
     6781 gagcataata atcggcaggt agagaaagag catgaccaag gcaagataaa tgcctgaggc
     6841 ggggtgaatc ttgctgtttt tcatgctaca acacctcccg cttcctcggt atcgccgaat
     6901 ttattcataa cagccataca gataagtatg agaatcataa gaacaagtga aagtgcggct
     6961 ccgaaattga agttgtttga aacctggaac tgcatttcga ttatatcgcc tatgagcgca
     7021 aagctaccgc cgccgagctt ctgagaaata tagaatgtgc tgaccgacgg aacaaagacc
     7081 attgtaacgc ccgagacgac acccgaaagc gttgacggga aaataacgtc cttgataaca
     7141 tggaatctgt ttgcgccgag atcctgtgcc gcttcgataa ggctgttgtc aagctttgtc
     7201 ataacggaat aaatcggcag aatcatatat ggcaggaaat tgtaaaccat tcccaatatg
     7261 accgcacccg aggtgttgat catgtggaag ggtccgatat ttatttttgc gagtatgctg
     7321 ttgataacgc ccgaatcctc aagcaaagtc atccatgcgt aggttctgag aagaaagttc
     7381 atccacatgg gaatcatgac gagcattacc attgtgctct gagctctgag cttcattttt
     7441 gagagaatgt aagccaaagg ataagcaatc aaaaggcaga tgatcgttgc aacgataccg
     7501 aaccacaaag aacgcagaaa cgtgcttgtg taattggtca ggcgtgtaac attgtcaaga
     7561 gtaaatgcgc ccgacttatc tgtaaaggca tagtaaaata ccattataag cggaacaacg
     7621 atgaaaattg ccgaccagag tatatggggt gcggcgcaca aacgctgagt taaagtcctt
     7681 ttattcacgg ctcattcctc ctcaacgatg tcggaaagat gctccagttc atcgctgaag
     7741 gagctgtaat ctccgtacag tcccgaatat ttggattttt tcataatatg aatagcgtcg
     7801 ggttcgattt caaggccgat ttttttgccg acagcctcgt aatcggtcgt ctggatcatc
     7861 catttgaagc cgccgatatc aacaattatt tcaaaatgaa cgcccttgaa cgtaatggaa
     7921 gtaaccgttc cgctgagcat acccttttcg acgggaacaa tatcaacatc ctcgggtctt
     7981 acgacaacat cgacggactc gccgatgccg aagcccttgt caagacactg gaactgtgcg
     8041 ccggagaagg agcacttgaa gtcctcaagc atcttgccgt caagaatatt actctcgccg
     8101 ataaagtcgg caacgaaagc gttcttcggt tcattataaa cgtcctgcgg agtgcctatc
     8161 tgctggataa cgccgccgtc cataacaacg accgtatccg acattgaaag agcctcctcc
     8221 tggtcatgtg tgacatatat aaacgttatg ccgagtctct gctgaatgtt tttaagctcc
     8281 acctgcatat ccttgcggag cttgagatcg agcgctccga gcggctcgtc aagaaggagg
     8341 actctcggct taacggcaag cgcacgggca attgcgacac gctgctgctg accgcccgaa
     8401 agagaattga tatttcgtct ttcaaagccg ttgaggttga caagattaag catttcctta
     8461 accgtctcgc taatttcctt ttccttcatc ttcttgacac gaagtccgaa cgcaatgttc
     8521 tcgtaaacat tcaaatgcgg aaacagcgca taacgctgaa aaatggtgtt tacctgtctc
     8581 ttatgaggcg gtacaccatt gataacttta ttctcaaaga caacctctcc actgtccggt
     8641 tcaaggaatc cggcgatgat acgaagtgtg gttgtttttc cgcaacccga aggtcccaac
     8701 aaggtaataa attccttgtc tcgaatgtac atgctgagat tttcgagtat ctgagcatct
     8761 cccagcttga ccgaaatgtt tctgaggtca ataataacgt tgt
//
LOCUS       DAWBSW010000076         8691 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1656_length_8691_cov_2.483905, whole genome shotgun sequence.
ACCESSION   DAWBSW010000076 DAWBSW010000000
VERSION     DAWBSW010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1656_length_8691_cov_2.483905"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    449..458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6433..6442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2352 a         2102 c         1798 g         2419 t
ORIGIN      
        1 ttttccggca tggacatttt ttcgtcgccc tttgtcatgt gaacgggctt gagtttgata
       61 acgccgttta tttttttctc accgaccgtt accgctctgc cgagcataac acgcttgtca
      121 attccgccga ttctctcaaa accgagagtt ccgtctgaat ttatgcttgt tatcataaat
      181 ccgacctcgt ccatgtgagc gtcaagcatt accttgtttt tcggagtttt cctgcccttt
      241 ttaaacacaa taaggttgcc gagcggatca acctcatagc tttcggcaca gtcctttatt
      301 tcatttatta taaactctct tacgctgtcc tctctgcccg aggttccgtt gagaagtgaa
      361 agcttcttta caagttcaag tatcgagacc acctccgaga atgtattccg caatcaattt
      421 tgccgtgttt tccacatcgt caacggctnn nnnnnnnncg tttgtaccgg ttgaatctcc
      481 catcagctca agctgatacg gtattttctt tgcctcggca gtcgccttga gcttttgact
      541 tatcctgtag tcaagcacgg gcgcaatgcc tatcatcggg cctttattga gcttgccgca
      601 cttttcgctc ggcacatcgg gcgcatcggc aaagctgacg tcaacggcta tgcactcatc
      661 ggcgtcgagg ttaaagctgc ccgttatcgc accgtcgccg cccgtctcct cctgaccgga
      721 gaaaaggagc ttcacgctcg gtctttcctt ctctttcgag acgagctcgg cggctctgat
      781 tatcgccgca cagcctgcgc ggtcgtcgag agcggcgccg aatattctgc cgttttccat
      841 ctcgccgtaa ggacatctta ccgttattct gtcgccttgt gaaataagct tctccgcttc
      901 ctcctttgtc attcccgtgt caataagaat cgcatcaaaa tccttggctt tcttaacgtc
      961 ctcgggggtc gagagatgcg gaggagtcga ggttacaacg ccgtaaacag gctctttgcc
     1021 ccaaacagta acgtcctgag cggcaagaac tcttgcgtcc gttccgccgc actttgcaac
     1081 acgaaggaag ccgccgtcct caatacccgt gacgatcatt cctatcctgt ccgtatgtgc
     1141 gtcaagaaga acggttttct cccctgccgg catgtccgca gtaacattgc cgaatttgtc
     1201 ctttttaacc tcggcaaatt tcgacacata tttttcgatt atatttgata tttcgctttc
     1261 cgctccgggc gttccgttcg cctcggaaag gtcgaaaatc atatccttta aagtgctcat
     1321 tttttcacct catttgtata tacgcgtata ataactatta tattataacc tttataaaca
     1381 aaatgtgcaa taatatttga ttttcttttt aaaatagttt ataatgttaa aaaaccacac
     1441 ggaggtctat ttaatggacg agaataaata tcaggttatt gatccgacgg atgacagcta
     1501 caagctcaca gctgccgatc tgaaaaaaga aaaaaagaaa aagcgcaagg gtctcaaggt
     1561 gtttgtcgcc attatccttg tttttgcaat acttgcaggc ggatcgggaa tatacgtcta
     1621 ctcctacctc aacaagctca attacggcga ttcgcaggga acggcaaacc cgaaccttga
     1681 caaagaggag tccctgagct ttgataatca ggcggacgcc gatgccgatc ttcgagcaag
     1741 actttccaac aacgttattt ggtacgacga cagaattgta aatattcttt tggtcggcgt
     1801 tgactacggt gacgaggaag cagtcatgtt caagggcgca tatctccccc gttcggactc
     1861 catgatcctc atctcgatca acacaataaa caatgtggtc aacatggttt cgctctcccg
     1921 tgcggcatac gttgcaatcc cgggtcacgg caacaagcga ctgaacacgg ctcatgccta
     1981 cggcggagca agcctgcttg ttgacacaat tgagcagaac tataagataa gaattgataa
     2041 atacatcacc gttgatatct cgggctttgc ccagattgtt gacgctctcg gcggagttac
     2101 gattgagatg accgcggcgg aagcgggcaa ggttctcggc gtttcggcag ccggaacata
     2161 cgaccttgac ggagacgagg cggtttacta ttcgagactc cgttcaatcg acacggaccg
     2221 taagcgtacg ggcaggcaga gagctgttct caacgctatc gctttaaagc tcagaaaatc
     2281 ctcggcctcc acaatggtag accttcttga cacggttctg ccgcttgtta caaccaattt
     2341 ttccaagacg gagcttctcg ctcaggttgc aaaggcaccg aaatatcttt cgatgagcat
     2401 acatgaggac atcattcccc atgttcctta tccgctttca acccgtgacg gcaaggaagt
     2461 tcttatcctc aactgggacg aggaggataa atacattcac gatcttcttt atcccgatat
     2521 cattccccag tcggcagaaa aagaataaaa ttcaccgtac cgaagttaaa agcaaggctc
     2581 ggtacggttt tttgatgtgt tgcggtaaaa tttctatctt ggctcccttt taggggagct
     2641 gactggggtt ttattgaaaa tctgataaga tcttgtttaa cctcccatgc atgggaggca
     2701 agttaggtgt ctgccttgaa aacaatttca tcaaaatttt aaaggtgcga tgtgggcatc
     2761 gcaccctacg acgaggtgca gaaaagcaaa attctgcaaa agtgtggtaa gagaacattc
     2821 cgtaggggtc gatgcctaca tcgacccaag ttgagaagaa aattcttggg tgcccctaca
     2881 gtgaagccaa aaaaattttg caaaaaaaat aatttctcac cgaaaaactt cacttcttac
     2941 aaaactgtaa gatttgagag ccgttttgta agacaattct atggagatac gttccttctt
     3001 tctgatatac taacgtcaga aaattgattg gaggtcaaaa caatgagtat tctggaagtc
     3061 aaaggactta aaaaaatcta ttcaacacgc ttcggcggca ataaggtcga ggcgctcagg
     3121 aacgtaaact tcaccgtcga ggagggcgaa tatgtcgccg tcatgggtga atcgggatcg
     3181 ggtaaaacaa cgcttctcaa cattcttgcg gcccttgata aaccgacgaa cggcaacgtt
     3241 gttcttgacg gcaaggatct ttccaagata aaagaaaaag acgttgctgc attccgccgt
     3301 gacaatctcg gcttcgtatt tcaggatttc aacctgcttg acacattcac acttgaggac
     3361 aacatctatc ttccccttgt tcttgcaggc aagaactata aggaaatgag cggcaagctt
     3421 gaaacagtcg caaaaaggct tggtattctt ccgcttctca agaaatatcc ctatgaggtt
     3481 tccggcggac agaagcagag agccgcagtt gcccgtgcga taatcacaaa tccgaagctt
     3541 atccttgccg acgagccgac gggtgcactt gattcaaaag caacggacga attgctccgc
     3601 cttttctccg atatcaacag caccggtcag accatcgtta tggttactca ctcgctcaag
     3661 gcggcaagct cggcgaacag agtccttttc attaaggacg gcgaggtttt ccatcagatt
     3721 tacagaggca aggataccga cgaacagctt tatcagaaga tttcggatac cctcactctg
     3781 cttgcgacag gcggtgaaaa ataatgaaaa aaggatttta ccccaagctt gcctttgacg
     3841 gcattcgcaa gaataaaaaa atgtacctgc cctatattct cacttcaatg ggcatggtaa
     3901 tgatgtacaa tatcattatt ttcctacagt tttcacagag tattaaggac gcagtgcaaa
     3961 gctcgacagt cacccaaatt ctcggtctcg gctcatgggt catcgcaatt ttctcatgca
     4021 tcttcctttt ttacacaaac tcgtttctta tcaagcgacg aaaaaaggaa ttcggacttt
     4081 acaacattct cggaatggac aaacacaatc tcggcataat acttttctgg gaaacactca
     4141 taatagcggt tatttccctc gtcataggtc ttgctgcggg tatcgctttc tcgaagcttg
     4201 ccgagcttgt atttctcaat ttgttaaaaa gcgacgcaac atttgacctt acggtttcaa
     4261 gccagggcat cggactttgt gcaataattt ttacggtaat taacattctc cttttcttca
     4321 attcacttcg tcaggttcgg ttctcaagcg caatttcgct tgttaagagc gagcaaacgg
     4381 gggaaaaacc gccaaagggc aattggattt tcggtattct cggagtactt ctgctcggcg
     4441 gtgcctatta catttcgctc agcattaaag agccgctcca ggcgttgacg ctcttctttg
     4501 tagccgtcat tatggttatc atcggaacat atctcgtcat gatttccggc tcggttcttt
     4561 tctgccgcct tttgcagaag aacaagatgt attactacaa accgaaccac tttgtatccg
     4621 tttcctcaat ggtttacaga atgaagcgca acggtgcagg ccttgcttcg atctgtattc
     4681 tcgcaacaat ggttctcgtt atgatctctt caacgacaag cctttatttc ggtgaggagg
     4741 acgcacttgc aatccgttat cccagggaga taaacattga catttcgact cttgataccg
     4801 atcttgagaa aaacggacag atggataata taatatctga cattaaaaac atatgcaaaa
     4861 atcacgatgt taaaggaact gacgtctata cgtatctcaa cggcatgatc agcggaatga
     4921 taaccgacga tggcaaggtt gaaacggaca taaacagaat tgataacttc tccaatatag
     4981 ctctcggaga tttcagcagt gtttattcat tcatattcat cccggtcgat gattataata
     5041 aaatcatggg cacaagctac acgcttaagg acgatgaggc aattatctat aattatcgta
     5101 acgattcacg ctataaaaac aacgtaatat cctttaatcg gggtcagagc tttaaaattg
     5161 tcaaagagac ggatgagttt gttattgata ccaatgcggc aatatccgtt actccctcaa
     5221 tggcactgat tgtaaacgat attgacaacg caacaaaagg acttacttat aacgctacca
     5281 cggctcaaaa aatcgaaaat accggaataa gctttcacag gtattattgc tttgatacag
     5341 gagtttctcc gcagcaccaa atcgagcttt ccaaggatat cgcaaattat ctggacggaa
     5401 acatagaaaa atacagagcc gatacatcga gccgtatgtt ccgctcgtct gttgaaagca
     5461 aggaacagaa ccgattggat ttcttcagcc tgtacggcgg acttttcgga ctcggaatca
     5521 ttctcagcat agtattcatc ttcgccgccg tcctcataat ctactacaag cagatttccg
     5581 agggctacga ggatcagtcg agattcgaga tcatgcaaaa ggtcggcatg acaaaaaatg
     5641 aaatcagaaa gagcatcaat tcacagctcc tgacggtatt ctttctcccc ctcatcttcg
     5701 cgggaatgca ccttgctttc gcagcaccga taattaaaaa gcttcttctg atgttcaaca
     5761 taacaaatct taatttcttc attgtaactg tcgttatcag cttcgcgata ttctcgctgt
     5821 tctacatgat tgtttacaag atcacctcaa gcgcctatta caggatagta agcggtgcaa
     5881 aggagggctg acaatgaaaa agctgcttgc aattttcatc gcagccgtta tcattctcgg
     5941 tgcagtcgag gtcgtcagaa agccgtcggc acagcagatt gtcgatgatt ttgacagcat
     6001 agttcacgtt atcagcctca cgcagataac aagagattca aagctcatcg gaaagagaga
     6061 aaatgtcggc aaggatacct atatcggcag ctattcctcc tcctgcattt cggagaacgg
     6121 acgtgacgtg gtgttcggcg gagcatcggt caaggacaga aaaatcaagc tcaaagtgaa
     6181 aattcttacg gaaagcggag ccgcctctct cagggtcaga acaggctcgg acgtcaagga
     6241 atattccgtt gatgagaatg gaattattga aaagacattt gattttgaca gcggcggaaa
     6301 ctatgcaatg ataatttaca acaacttcac cggaacggtc gaaatgacat cggaatatgc
     6361 gaaatgaaaa agctgaaaca tattttttca aagcttgtat tcttcggcgg actcgccgca
     6421 ataggcatac tcnnnnnnnn nnggcatact caccgcccct gctgcaatgc ttttcggaat
     6481 gatagcgttc attcgtgcca tgaccgattt gataacaaat aaacttgata aatgaaaaag
     6541 gactgcacag cctctctcgg ttgtgcggtc ctgctgtttt aatatttcag tctcccctgc
     6601 agtggtgcca agaatttttt cttctaacct tgggtcgatt cggaaaagtc aagaaagtgc
     6661 ggtcggaaaa tgtcctaaaa accgaggctt ttttatattt acaccttcgg aatacgaaca
     6721 agaaattcac tgccctgccc cggctcgctc ttaacgcata ccgagccgcc gtgaaatgag
     6781 acgatctcct ttaccatcga cagtccgaga cccgttccgc cgccgttatg cgaagggtcg
     6841 gtctgataaa atctgcggaa tattttttcc tgctcgtcct ttgctattcc tattccgtta
     6901 tccttaacgc acagcattgc atttttgctg tttgaaagaa gcgtaatatt tattctgccg
     6961 ttttcaacgc cgtatctgta tgcattggaa acaagattac tgacaagcct tgtcagcagc
     7021 atccggtttc cttttactct tattcccggc tcaacctcac attcaagagt tatatttttt
     7081 gttttgagca acgcaaggtc gtcgcaaaga ttttcaacaa gctccgagaa atcaagttct
     7141 tcaacattat acttttcgct ccgtctttca attcgtgaaa attcgagcat ttccgttatc
     7201 attttggtca ttttactgcc ctgccgtctg ataagctcta tcgactcctt gtattcctcc
     7261 tgcgtcattt cctcgccgag cgcatagtcg cactgagcca taataaccgc catcggcgtt
     7321 cttaattcgt gcgaaacgtc gtttgtaaat tgcttctcgc tttcaaagga atcctccagc
     7381 ctgtcaaaca ttgtgtcaaa tgtatttgca aggctgtgaa gctcatcgtt acccttgccg
     7441 agctttattc tctttttcaa atcgccgctt tgcgaaatat cacgggcaac ctcctcgata
     7501 tcctttatcg gtttaagcga tttcatagca ataatatagc cgccgccgat tccgagaatc
     7561 acgagaacaa taagcgcaat cattgaacgt cttgcaacat cggaaatagg ctcgtcggct
     7621 ttttcatgac tgactattcc tcttatccac atattttcga cgcccctgtt ggtgagacgt
     7681 cggtcgtaaa tatagtaacg ctttccgtca acatacacgg ttttcaagcc cttgtccgaa
     7741 aaagcttcgc cgtcggtctt tttgttcgta gggtcggtgc cgtaaatctg tactccgctt
     7801 tcatcatata taaccgaata aatgccgttc ataacgccga taaaatcgtc gtcgatttca
     7861 acatagccgt ctttatacgc aaaataatga tcattgtcgt tgtcatcaat cacgccctga
     7921 aggctgtcat aatactctac ctcgtcaacg ttatcctcaa ccgtcataat taaatcattg
     7981 cgcaggtttt tcaaaagcac ggaattgctg accgatatca caagaacata ggaaagcgtt
     8041 acaagaaata tcagcatagc cgaaaaccat aatgtaattt ttaattttaa aggaagcttt
     8101 ttcatcattc gtcacttatc acatatccga aacctcggac ggttttaatc agttttttat
     8161 caaattctcc gtcaaccttc ttgcgaagat agctgacgta aacatcaacg aggtttgttc
     8221 cgccctcata gtcataattc cagacgtggt tttcgagctt ttctctcgaa agaacaattc
     8281 cgacgtttct catcataaat tcgagcatcg aatactcctt tgccgaaaga gtgattgact
     8341 ttccgtcacg tgtcacctca tgagttgagc agttaaggct caggccgtca accgagagaa
     8401 cattctcgga aactccgtat tttattctcg tcatcgcccg tacccttgcc atcaattcat
     8461 caaaagaaaa aggcttgacg agataatcat ttgcgccgag gtcaagcccc gtcaccctgt
     8521 cctcaatcga atcccgtgcg gtaaggaata aaacgggagt gcttataccc ctgcttctca
     8581 tttctttaat aacggcaaag ccgtcctttt tcggcatcat aacatcaaga atcaccgcat
     8641 catattctgc aaattcaaca aaatccaacg cttcctcccc gtcaaaggca g
//
LOCUS       DAWBSW010000077         8668 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1662_length_8668_cov_2.416231, whole genome shotgun sequence.
ACCESSION   DAWBSW010000077 DAWBSW010000000
VERSION     DAWBSW010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_8668_cov_2.416231"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7922..7931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2242 a         2007 c         1973 g         2436 t
ORIGIN      
        1 gttctccttt tttcttgaat cgagggattt tctgacgatt tcaagtgaga ggattttcct
       61 tttggaaatg cgcagagctt ttgccgcacc tgccagcaca tcgtcatatg taccctccaa
      121 cggagttttg atttcattaa ctcttatcat ataattatcc tgttaaaaat ttttggtata
      181 ttttataatt cgcatctcgt ggttctgcaa attatacatt acttggtaaa ccgtgtgctt
      241 gcccgagtcg ggaagcgtgt tgtatgcctt tgactcgcag tcataaaaaa taaagaggtt
      301 gtatcccgag ttgtcaacga gcgaatattt caaaccattg attttgtccg gaatttcgct
      361 gagccacaaa ggggattttt taataaaatt ttcaaattcc tccgcctgct cgggcttata
      421 aattacatcc gtgcatgaat agagcttctc tctttttata aatgacttta acgaagcgac
      481 agcgcttttg ttttcgtctt tgagtcctat actgtcaacg gtggttgcgc tgccgtcagc
      541 cgggaaagca attccgatta tgttccctgc ttccgtgaca tacccaaaat cagatgaaaa
      601 gccgtttttg ccgagtgtaa tttgctttga atctatcggc gaaaaaatat tgaaaatctg
      661 cgccgcacaa aaagcaaaac aaatcagctt gataataatt tcgctgccga ctctgcccat
      721 ataataatag ataaaaaata gcgagccgag cggaacgaga agaaaagcaa gcggtatcca
      781 aacgggcgtt gtcccgtgaa cactgtacca gctttgcaag aatgaacggc tgaaaataag
      841 agccgcaagg ttgacaacaa caagcatgat tctcatcaga acaggcgagc ggtattcaac
      901 attgaactgc gggtcaaact ccggcggacg ttcgtaaagc tcatcgccgt aagcagaccc
      961 gttaatttcg ttttgaggca atagcttcac cgtccttgat ttaattgccg atattttcag
     1021 cacaatgctc ggctccctgc atcgtctgca tagggagcct gttttttatt taatagccat
     1081 gagaaaagtg ggtttagttt tacgctttaa agaaagctgt gaaagccttg tttgccatgt
     1141 aaacgtcgag cttggcgatg agctcgaacg gagcgtgcat cgagagaacg ggaacgccta
     1201 cgtcgataac atcaacgtca aggtttgcga catactttgc aaccgttccg ccgccgccgg
     1261 cgtcaaccgc gccgagctcg ccgagttgcc aatttacgtt agcgttgttg aaaattctcg
     1321 taacctcgcc gacaaattct gcacttgcgt ccgaggtgcc gcccttgcct cttgcacccg
     1381 tatacttcgt gacaacgatg cctctgttaa gataagaagc gtttcttgcc tcgaacggag
     1441 tcgggaaggt cgggtcaaat gccgcattga catcggcgga gaggcacttt gattttgaaa
     1501 gaacatccca aaccataagt ccctcggatc ttgcgaggtc ggcgatgaaa tatttcatat
     1561 atgatgaatt caggcctgtg tttccgtctg agcccgtctc ctccttgtcg gtgagaacgg
     1621 caacggctgt atagtcgggc gtttcgtcaa ggtcaaggat tgctctgaac tcgggataag
     1681 cgcagacacg gtcgtcatgg ccgtagccgc cgataagact gcggtcgaag ccgatatccg
     1741 tgacctttgc cgcaggaacc atttcaagct cagccgtgag gaagtcctcc tcgaccatgc
     1801 cgtacttttc gttgagaagc ttgagaatgt taagcttaac gagctcgctg cccttgtccg
     1861 ccttgaacgg aagcgagccg acaataacat tcagctcctc gcctttaatt ccgtcgtcaa
     1921 gggttctctt gtcctgctcc ttcgaaaggt gaggaagaag gtcggatata acgaactgag
     1981 gctccccttc tttttcgccg aggctgactt ctacctccgt gccgtcacgc agaacgacta
     2041 tgccgtggag tgacatagga atcgttgtcc actgatattt cttgattccg ccgtagtagt
     2101 gagtcttgaa aagagcaacc tcgtttgctt catagagagg gttaggctta aggtcaagcc
     2161 ggggagaatc aatatgagcc gctgcgatcc ttacaccctc gccgacgctt tttttgccga
     2221 taacggcaag gataaccgct tttttacggt tgagatagta aaccttgtcg ccgggtgcgt
     2281 acttcttgaa tttatcaaag ggaacgaagc cctttttctc tgcctcggca acaacaaatt
     2341 ttgcgacctc tcgctcagtt ttgttatcgt tgagaaattt tttgtaatcc tcggcgaaat
     2401 ccatcgcctt ttgaagctct gcttcatcaa caaaatttgc gctgtgcttt ttgttcataa
     2461 acaatttttc gcacaaaaca tcaacttctg tcttttcttc tgccattgtg atttcctcct
     2521 taaaaattcg accgatttac ggtaaactta ttctttttcg ccttgcttga accgacagtt
     2581 tgtgcagggc gtttgtttta taattcattc agttgatcgg acaggttttc gcttccgtta
     2641 ttgcggacaa taatatctgc cttttctata taatattgct cgtttttttg tgcatttatt
     2701 ctgagcatgg ctgtctcgcg gcttatgccg tcacgcttca ttactcgctc ggcacgtata
     2761 tccctgtcgg cggttacaac tatcattttt gtgcagtatt tcggcagatc acaatcgaaa
     2821 atcgccgccg catcaatgat aacgcccttg tattccgatg tgaaattatt ccttattgtt
     2881 tcaagcgcaa gagcggttat cacaggatgg gtgattctgt taagacgctg acgcttatgc
     2941 tcgttggcaa atgcacgctt ggcaagctca ctgcgaataa gattgccttc ggaatcaaga
     3001 atttcttttc cgaattcggc ggcaagcgca ttgagaacat cgggaatttc aacagccctt
     3061 cttgcaagaa tatcgccgtc aactatcaga tagcccttct ttttcagctc attgccgact
     3121 gtggttttgc cggcgccgct ctgacctgtc agaccgtaaa taacgggcgg ctcgataatt
     3181 ttgaatgccg ctgcacgcaa aagacggttg taaactccga gacctactcc ctcggcggac
     3241 ggtgcacgga cgaaaacacg ctttattccc atatcgtcaa atttacgcaa atcatcaaaa
     3301 aggctgtgcg cctgttcgag cgaattatcc tttctcccga acgaaaggca gggaacggga
     3361 agttctttct cttcaccgtc aaagcaaaga gccgcatctc cttcacgcaa aacgcagtca
     3421 atctgatatt taaagctcgg cagactgccg tttactatat atacctcggc tttcggcgaa
     3481 taatgcttat atttcattcc cggagaagcc ggacgctcgc cttccttgag ctgtgagagc
     3541 acggcagggt cgatattaac gtgacccagt accgcctcga gctgctcatg ggttattccg
     3601 ccgggtctga gaagtctcgg cggatcggtt gcaagcgtaa cgaccgtgga ttcaatacct
     3661 acgtcgcacg ggccgccgtc gacgattgct tcgatttttc cgttcatgtc gtccataaca
     3721 tgttttgccg tcgtcggact cggaagtccc gaggaatttg cggacggcgc ggcaagcgga
     3781 cgaccggttt tctcaattat cgctcttgct acagggtgag acggcatacg aacagcaact
     3841 gtcggcaggc ctgcgctcac ctcgtcggga attatatcgg atttcggcaa aattattgtc
     3901 atcggacccg gccagtagcg gtcggcaagc tcctttgcct tgtgcggcac acccttgaca
     3961 agcctgtaga tttcctcaaa acggctgata tgtactatca gcggattgtc catcggtctg
     4021 ccctttgctt taaagatttt cgcaaccgca tcggaattca gcgcgtccgc cgcaagtccg
     4081 taaaccgtct cggtcggaat tgcgacaagc ccgccgcttt tgagaatttt agcggccttt
     4141 tcaatatcct tctgtgagcc gccgtcaaaa atttcagttt ccatgttctt actcagcttt
     4201 cagtttttcg gcagtgtctg cggtgatgag agcgtcgata agctcgtcaa gatcaccgtt
     4261 cataatagcc tctattttat ataaggtcaa cccgattctg tggtcgctga cacgaccctg
     4321 cgggaagttg taggttctta ttctctcgct tctgtcacct gtaccgacct gcgattttct
     4381 ttcgcccgcg atcgcttcgt tctgctttgc acgctcctgc tcaaggattc tcgaacgcag
     4441 aattttcatc gctctgtctt tatttttata ttggcttcgc tcgtcctgac attcaacgac
     4501 cgtgcctgtc ggaatatgtg taattcttat tgccgattcg gttttgttga tatgctgacc
     4561 gcctgctccg cttgaacgga aggtatcaat ctgaaggtcg gtcgggttga tttgcaggtc
     4621 aacctcctcc gcctcgggca gaacggcaac ggtaacggtc gaggtatgaa tcctaccctg
     4681 cgattccgtt tcgggaacac gctgaacacg gtgaacaccg ctttcaaact tgaagcgaga
     4741 gtatgcaccg tctccggata tcatgaaact gatttcttta tacccgccaa gctcggttgg
     4801 atttgcattg agaatttccg ttttccagcc cttagcctcg gcatacatcg aatacattct
     4861 gaaaagagaa tttgcaaaaa gtgcggattc ctcaccgcct gcgccgcccc ggatttcaat
     4921 aataacgttt ttgtcatcgt tcggatctct cggcagcagc agaattttca gctcttcctt
     4981 gattttctcc agatcgtctc ttgactgctc aaactgctcc tgaaccatct cacggaagtc
     5041 tttatcgagt cctccctcgc cgagcaaggc ttgagcttct tcaaagtcgg cgtttacttt
     5101 tttatattct cggtattttt caaccacggg agtcaggtgc ttcgcttcct gcatgagcgt
     5161 tttgtacttc tctatgtccg aaacaacgtc cgggtcacag agctgcgagt ttatgctttc
     5221 aaatttttct tcaacagatt tcagtttttc tatcatagta agttcctccg aaaattattt
     5281 gttctcacac ggggaacgct aatcctcggg gtgaattatt ctgcgaatat ttttttctgc
     5341 cttagagcgt ggaaccatga cctcatggcc gcatttttcg catttgattt taaagtccat
     5401 gcccgagcgc aaaacatcaa aggtattaca gccgcagggg tggtttttct tcataacgag
     5461 cttgtcgccc acacgaacgt ccatgcaatc gctccttttc aataatttct ccattatatt
     5521 ataacacatt ctgtgccgaa agtatagagg tatttgtcat tatcgggaat agaaatcata
     5581 taaattagta aagtaaccgc taacaagcag tcactaaaaa acaaatttaa ataaaacaaa
     5641 ggtggtaaaa cttatgcaaa actttaaacc ggaaggtcag actattctca ctccgtccaa
     5701 tcaggctgcg ctttcttcgc cgaattcttt gcacgaggct tgtatcgtcg gagatacgct
     5761 tgaagcacgg gctttgcttt gcgacaagga gcataatctg cgtgtcgatc tcggttgtat
     5821 gtacggaata attccccgca acgagggagc gctcggcatc gacaacggct cggtcaggga
     5881 tattgcgctg atttcgaggg tcaacaagcc tgtcatgttc aaaattatcg gtttccaaaa
     5941 ggacgataac gaacgtgaat atgcaatcct cagtcgaagg gctgttcaaa aggactgtat
     6001 ggataattat atttcaaagc ttcgccccgg tgatgtgatc acagctaagg taacccacat
     6061 ggagaacttc ggcgtttttc tcgatatcgg tgcaggaatt aacgggcttt tgccaattga
     6121 cagcatttcc gtttcgagga ttccgcatcc gtccgttcgt ttttcggtcg gacagacgat
     6181 aagagttgtt gtcaagtcaa ttgatgagca aggtcgcctg acattttctc acaaagagct
     6241 gctcggcaca tgggagcaaa atgccgacga atttgctccg ggcgaaaccg tgcccggaat
     6301 tgttcgctcg gttgaaagct atggtatatt cgttgagctg acaccgaatc tggcaggcct
     6361 tgccgaatat tcctccggca tcacggcagg cagtcatgcg ggtgtttata taaaaagtat
     6421 gctccctgaa cgcatgaaga taaaattgat aatcgtcgat gcgtttgagg cggaatatcc
     6481 tgctccgccg ataaagtatt tcaccgaact tgaccatatt gacagatggg tttattctcc
     6541 ggcagagtgc tcaaaggtca tagaaagcag cttttcttaa cttttttcgt ttctcgacag
     6601 aaaaaataaa ttcttgcatt ttcaagccac ttgtattata atataatcag aataatattg
     6661 tggtttttgt tgaaatggga ggatttttaa gaaatgatct gtgatttaca ttgtcatact
     6721 aagctctcgg acggctcgat gggcatagat gacattattg ttctggcaga aaaatgcggc
     6781 gttaccacct tagctgtaac cgatcttgat tgccttgcag gcacggtaag ggcacgtctt
     6841 ataggagaac gccatggaat aaaggttatt cagggcgtcg agatttccgc ttatgacagc
     6901 gaaaacgaca gggaagtttt tattctcgga tacatgagcg attcccctga ccgccttgaa
     6961 ggtctttgcc accgcaacct tgttgcgaga aaaagagcgg ctcagtttat gatgataaag
     7021 gcggctcagc gttttccgat aacgaccgac ctggttctca aatgcgctca gggctcaacg
     7081 aacgtttacc ctgttcatat aatggcagct ctcgttgaga gcggagttac gaccgagctt
     7141 ttcggagacc tttataagga gcttttttca aagggcggag acaagagtat tctcatcaag
     7201 ccgaagttct ccgaaatggc ggaggttatt caggcagttc atgacgcagg cggaattgcg
     7261 atactttccc acccgggcga ttatgaaggt acaaatattg ttgagaaagc aatggaagca
     7321 ggaattgacg gttttgaggc ttggtgtccc gagcatgacg aggcaacatc ggaaaagttc
     7381 ccttcgcttg caaagaaaaa cggtattctt gcaatcggag gcagcagctt ccgcggcaga
     7441 cacaacagat cctgcgtaac cctcggaaca attgttactc ctgaggataa gttcacagag
     7501 ttttctaatt ataaggcaaa aatccgccgt caaaaagcaa aggcggaata atcggagaca
     7561 cgagaaatgg cagaggacag agatattaaa atctatgagg gcgggaaaag ccgcgagctg
     7621 aatgatattc agcggatttc cgaggatatt gaccgcaaca agcggaacgg aaatatcgat
     7681 aaggcaaaag ctctcggaaa aaggcttgca aaaattcgcc ccgactgcaa aaagctcgga
     7741 cttgatattg gcagaatgcc tgcggctgag ctttattgcg tgcgtgtttt gctgactttt
     7801 actgcggaat atgctgtcca aaagtatgtt ttatccgata ctcttattga tgccgtaagt
     7861 gcctcaatgt atgattatct caaggctgag gaaacggggt attacgataa catttccgac
     7921 gnnnnnnnnn nctcaaaaag agcggcgata cagcaaaaaa tatcggcgaa cagtttgcac
     7981 agagatgcgg aataaacagc gatgaatacg tcaccttcgg tgcagacata ttcaataagt
     8041 cgcttgaact ttactcaaaa ataatcgacg aaaccaaatt tgtcggtgaa taacaaataa
     8101 aatataaagc tgttgcatgg aaccttatct gtgcaacagc tttattgttt tacggcgatg
     8161 taaagtattg taattacaat aaaaacgatc agcagcaatc cccatttttt attatcaatt
     8221 aaatacacaa tcagttttat aaaccaatac agcagggcta aaataaatcc tatcatagtt
     8281 tcacctttct ttaaattttt tcgggtatta caagatacaa cctgtatata taaccgcaag
     8341 gggtggtctg tcgacgtatt ctgattgtgt aagagatata aaatcacata taatattttg
     8401 atgaaccgtg atttaacctc actttatatt aaaaagcaga aaccgtcatg gctccctttt
     8461 aggggagctg tcacggagtg tccgtgactg aagggtttat ttatgacttg cctaaatcta
     8521 tcttaaccgt ggtctcctcc gttttaggag gtttagttcg agggaaaaga tataaagata
     8581 catataactg acagaaaaac gagcagagaa aataaaaaag cagcaaagaa agcaaaatca
     8641 agatatatcc acaaaaagga gaaaaggg
//
LOCUS       DAWBSW010000078         8658 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1665_length_8658_cov_2.797977, whole genome shotgun sequence.
ACCESSION   DAWBSW010000078 DAWBSW010000000
VERSION     DAWBSW010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_8658_cov_2.797977"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2774 a         1666 c         2006 g         2212 t
ORIGIN      
        1 atgatataca taccgatatt tgattgtatt tatctataaa aactaattta ataaaattga
       61 taaagaaagc aggtgaaaag taattaaata atgaattttt ttattcaata attgaggaag
      121 tgaatcaatg aaaaagaaga aagtgtgtaa aatgataacc gctgccgcat tgtcgttgtt
      181 atttgtgttt agtattatcc cggcatctgc cttggatagt ctgagcgttg aagagacggc
      241 ggcaaagata gtcagcgagg ttaccggaat gagggaagag tccgtaaaat acttcctgtg
      301 tgatgacggt tcatacatag cggcgaccta tgccgcaccc gtgcattaca acgaaaacgg
      361 agtatggaaa gaaattgata acaccttaac tccttcaagc aaatcgggag aaacggttta
      421 ttcaacaaaa ggcggactta acgttaccgt tccgagcgag ttgggaagcg gaaaaagatt
      481 taccgcgacc aacgggggct acaccatcag ctttggtgtg aaaagcattg ataacagctt
      541 gtcggcgcag gcaaaggttg tggagaccga tgcattgccg tcggttgtta aaatgaactc
      601 gacggctgaa atctctaatg gaaaggttac agcctcatca atggctcaaa aggccgaaac
      661 attaacggaa aagcaaaagg ttgagaagtt caataacgag caaatgacag ttgataaaca
      721 atcgggtgcg gttgtttaca agggcttcga tcagcagtct gatttggagt atattgtcac
      781 gtcaaacagt ctgaaagaaa acattgtggt ttataagccg caggacgaat atgtttatag
      841 atttgattta gattccgacg ggcttattcc gattgagcag ccgaacggtt caatcatatt
      901 ggttgaatcg gaagattctc aggaagcggt gttcacactg gacgcaccct atatgtatga
      961 cgcaaacggt gcggagagtt atgacattga gctttcaatt aaggaaaacg gcgatgaata
     1021 cgttgtaatg gttgaagcgg attcaacatg gctcaacaac agcgaaagag aatttccggt
     1081 tgtcattgat ccgacgtgga gcgctccgaa ttcaaagatt caggacgttt acgttatcaa
     1141 cggaacattt gcaaattcgc cacgttcaaa cacagaaatc agagccggca gaaatctgac
     1201 aaatatcgtg cgctcgtata tcaagctgac gttgcctact aatttgccga taggatattc
     1261 tctttataac agtacacttg tgcttaaaaa gcagaattat tttaaggtat ctaaggacat
     1321 tgatatccgt gcatacgact gtaagaatgt tgcttcctgg agtacgtcga gtatttcatg
     1381 gaataatcaa ccgtttgata actcaaacaa cggatatctt aacagcgatc ccgagttgtt
     1441 atcgtcagtt gcggcgaaat ctgattcgga agattatata tttaatatta caaaggctgt
     1501 tagcagatgg gaaaagggtg aaaccaataa cggaataatg cttgcatcgt cggatgaaag
     1561 cacaaaaact caggtcgatt tttattcgac aagagcaact gcgaccgaaa ctcgtccaaa
     1621 ctttgtgatg tattatcatg acccgtatgt ttcgttaaag aaatggacgc cgacgtgtga
     1681 atcgtcagtc agcagtactg taaatattct ttgtcgttta ccttggactg ttgaaattac
     1741 acctgattct ccatggctca ctgttacgga cttaaaggct aaaagcttca agctgaaagc
     1801 cgctgagaac accttagcgt ccgaaagatc cggtacggta acggtaaaaa catcattagg
     1861 taatgtgact tctgtaatag gcacaataac cgtaacacag tttggtgcag agccaaacat
     1921 tattctcaat acagagcaca tggatgtgaa gcacacaaat caaacaaaaa ccatttcggt
     1981 tgtttctaat tcaagttgga ctgcatcgag taattcggac tggattgata ttgaaaacgg
     2041 aaacggtatc ggaaattcta cttttaaagt gaaagtacag gaaattccga aaaatgacta
     2101 caatacaacc aattcacgaa tcggcacggt aaccgttcag gcaggaacgg tatctaagac
     2161 aataaccgta aggcaattcg acgaggcttc ggagtatttc aatccgataa attccgaggg
     2221 aatcggtgta acgaggccgt ctacggaata caatcatcct cttgctcaat gggcgatgaa
     2281 gcttgcatac tcggcatata tgccattgac aggacaactt tcgggtattg caccgggaat
     2341 gtttatggaa acaggcataa gaacggcaca ggaggagctt gaagattgcg gttttgagtc
     2401 aagaatttat aataatggtg aaaatgacac ggtttgtcat attatcggac ataaacaaat
     2461 ttatacaaat gtaagcaata atatatctta taatacaaat ggaggtaatg acaatggatc
     2521 tttgggaagt attgaaaaga ctatcggagg caaatccaat ggaagtttta atgatgattc
     2581 taaatccaat ttatggaccg atgtttctat gggcaatgtt ggggttaatt atagcggaaa
     2641 ttcagaagct tttgggaata ggatgaattt atctaatacg gactgtatgc gcaccttggt
     2701 ggttgtcgat ataaggggaa catcgacaaa cgaggattgg ttaactgacg ctggtacaca
     2761 gtttgaattt ttgggtataa attttgaaac aggaatgaac atggtattaa actctttatt
     2821 tcacggaacg ggagataccg aaaactgtac ggaatgtagc ggcgtaggtt gtgaaacatg
     2881 tgagggatat attccgcaca ataatattac caatccgatt ttccttgtta caggccatag
     2941 cttgggtgcg gcggttgcta atcttgttgc gaatcatttg gattcgtgcg aggacacaaa
     3001 ttgctgctca ggttcaagaa cggtagacga tgtttatgca tatacctttg caacgccgaa
     3061 aaccgttaaa aataagcagg gcaacgaata tcaaaatata tttaatattt tgaataacaa
     3121 tgatgttgtt ccgttagttc cgaccaatat acttgcgtta aattggggtg acaacggttg
     3181 gacaaggcac ggaagagatt tccatatcac tatgccgttg tatctgcaaa tggatttgtt
     3241 aaaaatcttt gattcggcac tcttaggtct tggcggtcat gcaatgtcca catacagtca
     3301 ttggctggaa aaattacccg gtatgcttaa taaaaaagcc gaagatatta cggcatagga
     3361 tttaaagtca ataagtgacg aacgggaagc agtgggatta ttacccaggt tgttcaaggt
     3421 aaagtgccct gtcgatgtta cgctgaaaga ctctgccgga aatattgtcg cctacgaaag
     3481 tgctcgtgaa aacgccgttt atcccgagct tgcggaaagc ggaattactt catggatatc
     3541 gaaagacaac gaaaaggtat tcttcctgcc ctttggctgt gaggacgtta ccgcagaaat
     3601 tgaggcgtat gactacggca caatgaatgt tgcgcttgaa accataggcg caggggatca
     3661 actggaaagt atgacataca gcaacgtcag cctctatccc ggcaaggatt ttgaaattca
     3721 gattgatgaa aattctgttc cgaatgagag ctgtcttatg gccgttgccg aggacggaac
     3781 tcaaacagaa ccgtcaaaga atccgtatct caaaagcgca gttcccgata cgccatatgc
     3841 gggaaacaat tacataactg ttgttacgga tcgaagtgtt acaaaagttc aatttgtaca
     3901 tcacgacagc agagatacaa tgacatacac aagggataat gttgatgtga cgcttgttga
     3961 tgacggcgaa accttgactt ggcgaattca tcgaaatttc ccagcgacag tatatgatgt
     4021 aggtgtgaaa gtcggttcat ataattggta ctatacagaa cgtgtttttg aattgaccag
     4081 agcataacac ctaagacata agctgcatta aagttgatca tggctttaat gcagcttaaa
     4141 ataagagaag tatattcaga ggaggtaaaa taatgaaaaa atacagcatt tttatgaaag
     4201 tgattgcggt tattctgacg gtcacgatga tcatcggaat attgccgatg acggtacttg
     4261 ctcaggctgt tgaagatgag aaagaaaagt ttgaacagaa cggatattca agcaatgttt
     4321 ctcaggagga agaggagcca accgtaatcg gtgaagatac tgagcgtcgc aacagcgaaa
     4381 atacaaagta tttcaagatg agcgacggca cgattaaagc tgcaatgtat aatgccccgg
     4441 tcctgtataa ggatggaacg ggtaagtggc aggaaattga caattctctt gactcctcta
     4501 atgaggttag taatgatgag gcgtcggatt tcaatggcta tgaaaccaag aaaaataagt
     4561 ttaaagtaaa gtttgcaaaa aatgcaagtc aaaaaaagct tgtgagcatg aagatggaca
     4621 actattccgt tagcctctca ttgctcaaca aaactaagaa aaacaactcc tcgatgaagc
     4681 aggaaaagaa agctaaaatt gaggatctta ctgttgcttc aaaagcaagt caaaaggttt
     4741 attacgagaa tatcttgcca gacacgaata ttgagtatat tgtcaacggt tcgggcgtta
     4801 aggaaaatat agtagttaaa tctgctcaga gcagctatga atataacttt gagattgctg
     4861 taaaggatgt tactctctct ttggcagagg acggttgtat ttatgccaag gacgcaacca
     4921 caggtacgac tgtatttgtt atgcctaagc cgtttatgct tgacgctaat tatgaatact
     4981 cgaataaggt cagctactgt ttgagtgcaa aaaacaagca caaatacgaa attacaattt
     5041 ctgccgattc cgaatggttg aacagcagtg aaagagcgtt ccctgtaacg attgacccgg
     5101 cgatacaaac aaaacagtca ataagcacaa ttgattcggt gtatgttgcc aaaggtttgc
     5161 ctaatcagaa tcgctattcg gatccgatga tgatggtcgg cagagagtca acagacgcag
     5221 atatttgcca aggtcttatt aaatttgctc ttccaaaaat aaatcgcggc gatgttgtaa
     5281 ttgatgcaca gcttttatca aagcaggtgc ttatggatgc gtattcagac actactcccg
     5341 atacggcgat agaggtgcat gccattacaa aaagttggaa cgttacaaca gtaacgtgga
     5401 atacgaaacc gacctacgaa tccaccgttg ccgactttga aattttgaaa gcaagcgaaa
     5461 agaattcagg agctaaggat cgacagtgga atgttacatc cattgtaaaa cgttggtatg
     5521 aaaatccaaa ctttgcaaac aatggattgt tgctgagatc atcgaacgaa aataaaacct
     5581 cgtataacga ttcctgcatt tatatgtggg tttatggcga aaagtatgcc caaagcacgg
     5641 cttatccaat gctttatatt acatacaggt caaataaagg acttgaatcg tattggagct
     5701 atacggatat cagcgcaggc agggcaggaa cggcgtcaat atgcgagttc tccggcaacc
     5761 tcgtatttac ccataacgat gtaacaactg ccggcagccg tgcgccggct tcgatttctc
     5821 acgttttcaa caactatatg gcagcggaaa acttcggtac cgtcatgccg tacagaggta
     5881 agggctggat gctcagctgt cagcagcagc ttctgccgtc atcaaaagtc ggtcttcctg
     5941 ccgatgcaca gaaaaaatat ccgtatgttt atatcgacgg cgacggaaca gagcattatt
     6001 tctataagaa aagtgacgga acaatggtcg atgaggacgg aatgggtctg actcttaccg
     6061 tccccggcaa ggataaaaac ggctattata ttgtcaaaga tgataagggt aatcagatga
     6121 tttttaacgt tgcaggtgca tttgcacggt cgcgtgacag caacggtaat cagattgcca
     6181 attactatgc cgccgccgac aatccgaaaa tttggaaggt taccgatggt gcgggtcatg
     6241 ctgtaacatt tacaccgacc tctgacagta aaacaattca gaaaataaca gattcggcag
     6301 gcagagttac cacatacaca tggaacagtg acggacgttt gactactata acatatcccg
     6361 acggaaaaga aacgaagttt acttatgatt ccgatggagc tatgacgtcg gtgattgcac
     6421 ccgacggata taagcttcag tttacttata cgccgttggc aaaaggcaag cgagtttcca
     6481 aggttgttga gagtgcaaac ggaacggcag gtcaaacaat aacatttgat tattcgcagt
     6541 acggacaaac agttattcgc tcttcgggta aagacggcgt ttatggcaac agcgatgatg
     6601 tctacacaac ggtcaagttt gacagcgcag gcagaaccgt gtgctcggaa gcgacaagca
     6661 acggcaagtc tttagcggct tcggcggcgg aatacactgc ttcaagtccc aatagcaacg
     6721 caagcaacat caagcagctt aaccgattga gtaaatccat gacaggcggt cagtatgtca
     6781 gaaactatct tcgtgacgga agcgccgata taagcggaac atggactaaa gctcagtgga
     6841 acggtacggc ggatttcgtc ggacaacctg ttgatacgga tcagctttac ggcagataca
     6901 gctattattt aaacagtaaa acctttagcg acaaagccgc agcgagagcc tatcagcccc
     6961 tttcgtccgg tcagttcggc gcaggaaaaa catatacctt ctctgcatgg gtaaagacta
     7021 aaaacattgt gccgagggac gcttcgattc cgttcggcgc ggagcttatg gtaacatatt
     7081 ggctatcaaa caacaaagcg gtcgatgtac attccgatgt gcttgtcgga acaacggatt
     7141 cgaccattaa caacggttgg cagcgccttg cggtaacatt tacacttccg tccgatgcca
     7201 caagaatgaa tataaatata atgattcgtg acgcaacggg tgaggcgtgg tttgatggaa
     7261 tgcagcttga ggagggcaca agcgcaagcc cgttcaatat gataaacaac tcctcatttg
     7321 agcggtattc tcaggcatct aacggggcat atttgccgca ggattggagc ggacatcttt
     7381 ttgattccgg agattctgta cataatattc atcaaaaaga tgacggacat acgttccgtt
     7441 tcaacagtgt gccgactgtt gaaaaatcat tgattcagac tctgtatttt aagggaaaaa
     7501 ctgcacaaaa tcttgacgat acatatattc tcagcggctg ggtatatgcg aacccggttg
     7561 gcggaagcaa tgaaaataac agagtaggtc ttgcgataaa gattttttac agtgacggaa
     7621 cgtattatgt acgttggttt gattacaaca attccgttat gggttcccaa tatgttatgg
     7681 gtgcatttac attgcgtgac ggaagtaagc cgactgcgaa taaaaccccg acttctatga
     7741 atgtctatct aatcaatttc aagcagtcaa ataacagctg gtttgataat attcagctcg
     7801 ttcgtgatga agccccgtca tatacctata acagcgacgg aaagctcgtt acggttgcgg
     7861 caaacgcaaa gcaaaagggc acaatggagt attccaacgg caaccttaca aaatctgttg
     7921 atgccaaggg ctttaattat tcatacaaat atgacgacaa tcataatatg accgaagcca
     7981 cgaaccagag cggtgttaag tatgcctatg tctatgacaa aatgggtaat cctacctcgc
     8041 ttaaagtcaa agtaaacgat aattattaca tcgaaacagg cgcaacgtac acagcaaacg
     8101 gcgcatatgt ttcgcagacc tcggatcagg acggctacac ggaaagctat ttgtataacg
     8161 acacaagggg tacgctcaca aaatatacag acaaaatggg tcgagtgacg aactacacct
     8221 atgataacaa taccgatgcg gttacgtctg tttcacaaac tctttcaaac ggtcaaacgg
     8281 tcagcaacag ttatacttat gacaattatc gtttgaaaac gatatcgcat aacggattta
     8341 attattcgtt cgcatacgat aatttcggca atgtaactca gaccaaggtc ggcactcagg
     8401 tgctcagcac gaatacctac ggcgcaaaca acggcgattt gaagcaagtc aagtacggta
     8461 ataacgatta tgttgactac acctacgatg attacggcaa catttccgca atttcgcaga
     8521 acggaacgaa gaatttcaca tggcagtatg actcaacagg aaatctgtac tctcacgaag
     8581 acttagtcaa caatcagcgc ttcgtttata catacgattc aacaggcagg cttgtccgcc
     8641 agcaggttct ggataaca
//
LOCUS       DAWBSW010000079         8608 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1680_length_8608_cov_2.190693, whole genome shotgun sequence.
ACCESSION   DAWBSW010000079 DAWBSW010000000
VERSION     DAWBSW010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1680_length_8608_cov_2.190693"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1638..1647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4800..4809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7520..7529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2247 a         2216 c         1697 g         2418 t
ORIGIN      
        1 ttttacaaag tccgtcaact ctcagaaatt ccattttcct caacaccttt agcaatagat
       61 tgaacaatat cgttcagaca ttcggctcgc tgcagtgcaa ttcccctgct tttgtcggca
      121 aggagaacga gcaaatcacc ggatttgagg ccaaatatat cccttacttc tttcggaata
      181 actatttgcc cctttgggcc gatctttacc gttgcaataa atttgtcgtt aagcattaca
      241 ccatctccgt tatacaagta ttacttttct tacttataat acttccgact ttaaaaattg
      301 tcaatgattt cagagaatat ttttttaaaa caatgacaaa actatctttg acgaaaacag
      361 gaggagataa catgattaac aagaatgaaa aaaagaacaa gtggcaaaac tcaaaggttt
      421 tctatgccgt tgccatgtca cttgtaattg caatagtcgg tgttgcatcc gtaatatata
      481 atctttcaaa aatcaaaaat gttctgcccg acaacggaca gagcggattt tattccgtca
      541 caacgggaga gagaacgacc gccgagcaca ttgccaacaa gccgcagacg ggagtaccgg
      601 ataatcgaga cataaccgac cgtacagagg aaaagacgac cgccaatgac ctcaaccgac
      661 cgtacacagg caaatatctt ttgccgctca attcaaccct cacaaaaagc ttcagtgacg
      721 gcaacgttgt cctttcaaaa acaatgaacg actggagaac ccatgacgga atcgacatca
      781 agagcaacat cggcgacaat gccgtggcaa tcaatgacgg aaaagttacc gctgtttata
      841 acgatgcgct ctggggtgac gttatcgaaa tcgaacacgg caacggactg aaagcaaaat
      901 attgcggaat caaatccgag ctgaaggccg aaacaacggt taagcaggga caggttatcg
      961 gcacggtaac tcagatcccg attgagaaag atgacggcat tcacgtccac cttgaaacct
     1021 tggtcgagga caagacggtt gaccccgtaa aagcactcaa cctcatgtcc gacaccgcaa
     1081 caacggaata aaaataaaaa ccgccgaatc ggcggttttt ggttttatat gtttgtcacc
     1141 tgtcaagctc aggtcatctt ttcctgcttg accttatggt cgattatgtg acgtgaagca
     1201 agttcgcggt gaacatcctc taccgaaatg cccatctcaa ccatgagaac cattgcatga
     1261 tatgcgaggt ctgcaagctc ataaacagtt tcctttttat catttgcctt gcctgcaatt
     1321 ataacctcgg tgcactcctc accgaccttt ttcaaaatct tgtcgattcc cttttcaaaa
     1381 agataggtcg tgtatgagcc ttccggcttc tcctcttttc tgccgacaag aaggtcatag
     1441 agtccctgca agctgaattc ttgcaattct tcgctccgat aaacaggctt tgtgaagcat
     1501 gaatccgttc ccgtgtggca tgccgggccg tccttttcta cgacaacaac aagtgcgtcg
     1561 ttgtcacagt ccgccgttat gctgacaatg tgctgataat tgccgctggt ttcgcccttg
     1621 agccaaagct cctgcctnnn nnnnnnntgc cttgaacggc tccaaaagca ggtcaagccc
     1681 ttttccattg agattttaag gctctccttg ttcatatatg caacagtcaa aaccttcttg
     1741 gttattgagt caacaaccac ggcaggaata agaccctttt cgtcaaattt aagctcatct
     1801 atattcaaca taatcataac ctcactgtaa tattattctt tctgagttca cgcttcaaat
     1861 cgggaatcgc aacctcgccg aaatgaaaga ttgaagcggc aagtcccgcg tcaatatcgg
     1921 gaatttcacg gaacaaatcg acaaaatctt taattccgcc tgcgccgccc gacgcaataa
     1981 caggtacatt gactgcgttg caaaccgcct ttaacatttc aatatcaaag ccttttttca
     2041 cgccgtcggt atcaatggaa ttgacaaccg cctcacctgc tccgagttcg acacagtgtt
     2101 ttatccagct tatcgcctcc atgccggtgt cctctctgcc gcccttggca aaaacatgaa
     2161 attcgccgtc aacacgttta acatcggcgg aaataacaac gcactgattt ccgtagcgct
     2221 ccgccgcttc acggataagc tcgggatttt ttatagcacc cgagttcacg ctgaccttgt
     2281 ctgcgccgca tttcagcact ctgtcaaaat catcaacggt atttattcct ccgccgaccg
     2341 taagcggaat aaagatctga cttgcaacct cacggagtat gtctgtgaat aatttcctct
     2401 cctcaaacga tgccgtgata tcgtaaaaaa caagctcgtc cgctccgttg tcggagtaat
     2461 actttgcaag ctcgacgggc gatgaaacat cggaaagtcc cttgaaattt acgcccttga
     2521 caactctgcc gtttttaacg tcaaggcagg gtataattct ttttgtaatc atctcgccac
     2581 ctcaatcgct tcttcaaggt cgacagcccc cgtataatac gcctttccga ttattgcccc
     2641 ataaagattc atttccctaa gccttttgac gtcctcaatg ctcgaaactc cgcccgaagc
     2701 ggttatgtca agcgaatact ttgccgaaag ctcacggtaa agctcaaggt tagttcccct
     2761 cattgcgccg tcacgggaaa tatcggtgca tattatattc ttcacgccga gatcctgcat
     2821 ctttaaaaag aactcgtcaa gcgtcacatc agactgttca agccagcctt tgacggcgat
     2881 tttgccgtcc ttaacgtcgg caccgacagc gattttttcg ccgtaagcct tgacggcttc
     2941 tttaagaaaa tcctcgtcgc atattgccgc cgtgccgaga atgacacgga aaatgcctgc
     3001 gtcaatatat ttcttcactg tctccatgct tcggattccg ccgcctattt caataaaaag
     3061 tcccgtcttt tcggcaacct cggcaacaat atccatgttc ggcgcagtgc cgtattttgc
     3121 gccgtcaagg tcaaccatgt ggatatgcgt tgcaccgagc gacttgaatt tttctgcaac
     3181 ctcaagcggt gaatcgctgt aaacagtcac ctgattataa tcacccttca gcagtctgac
     3241 cgctttgcgg ccgagcaaat ctatcgcagg aagtattatc atttgtataa tcgttccttt
     3301 cttgtgaaca tcttaatcct cgcaaaaagc ctttagaata gcaagtccga ccttgccgct
     3361 tttttcgggg tgaaactggc atccctttac gttgccgagc tcaactgcgg ctgtcatttc
     3421 tctgccgtat tccgttgttg caatcacgct gtcaccgcaa tccgtcgcat agtatgaatg
     3481 aacaaaataa acgcagtcgt tttcatttat gtatttaaaa agtccgctcg acctcgcaaa
     3541 atgaagagcg ttccaaccga tatgcggaat tttcagattt tccggaatcg ttccttccat
     3601 gggaacaacc gagcccttga gaagtccgag tccctcatgc tctccgtact ctaagctttt
     3661 ttcaaaaaga agctgcatcc cgaggcagat tcccataatg tccttacctg acgccgcctg
     3721 ctccttaacg acaatatcca taccgctttc acgaagcttt tgtgcggcat cggcaaacgc
     3781 accgacaccc ggcaaaatca gcttgtcggc agattttatt gtttcaatat ccgaagtaac
     3841 aaccgcctgt gcacctatgc ttgacaagga cgagcataga gaaaacaggt tgccgactcc
     3901 gtaatcaatt atcgctatca ttaaagcaac ccctttgttg acggaatttc atcggcaaac
     3961 tctttgtcaa tgctcaccgc cgtgcgtaag cttcttgcga ccgacttgaa tgaaccttcg
     4021 attatgtggt gggaattgct tcctgcaagc tgacgaatgt gaagccttat tcccgcatcg
     4081 caggcaaatg cacgccagaa ttcttcaaca agctcggtat caaaatcgcc gaccttttga
     4141 gtcggaattt ccagtgcata ataaagaccg cctctgcctg aaatatcgac agcggaaaga
     4201 atgagcgact cgtccatcgg aagagccgta tcgccgtagc gaactatacc ttttttgtcg
     4261 ccgagggctt ctttgaaggc cttgccgaga ctgattccga tatcctcaac cgtgtgatga
     4321 taatcaacgt aggtatcgcc gacgcacttt accgtcaaat caaatctgcc gtgacgtgca
     4381 aagaggttca gcatgtggtc aaggaatccg cagcccgttg tgatgtcagc cttgccgctt
     4441 ccgtcaagat taacattcag ctttatatct gtctcgtttg tttttctgtc gatatttcct
     4501 gttctcatct gaaacaccct ttcgtttttt caataccctt tttaagaatc ataagctcgt
     4561 ccatagctat gcaatttttg aagctcatgt tttgaacacc gtcaacacac tcattgaatc
     4621 caatccattt gacgctttca agctcttcct tttgaaccgt gaattcattc tcttccatat
     4681 cgcgccaaag aagaaatatt cttgaaaact gattgtcgtg aaacggcttt cccgaaaaca
     4741 catcgtccca ttgaacattt ctgtcgccgc aataaatcaa ttcattctca tctgtctctn
     4801 nnnnnnnnnc tttaagctca cggacggctg attttctgaa tccgtcgcct gccgggatat
     4861 gtcccgcact cgaaatatca tagcatcccg gatacgaatc cttgttctcg gcacgttttt
     4921 gcaacagtat ttcagctctg ccgtttcttt ttcttgcgag ccacacatga gatgttctgt
     4981 ggcgaattcc gtcggcatgt gcaaattctc tttcaacggt ttcgcctgtc ggctctccgt
     5041 tttcatcaac aatatctaaa atttcctgca tatttcaatc aatgatttcc ttaactgcgt
     5101 caataaaagt tttcatctgt tcggctgtgc cgattgtaat tctattataa tcgcaaatgc
     5161 gttcctttgt aaagtggcga ataagaatcc ctttttcttt aagcgttaaa taaagctcct
     5221 ctccgccgat tttatccgac tttgcaaaga tataattcgc ttttgacgga agaaccgtaa
     5281 atccgagctt ctcgagttcg gcggttgtcc atgctctgtt ttcctgaatc cttttacaat
     5341 tattcatata atagccgttt tcttcaagag ccgcaatacc tgccgccgcc gtcaaacggt
     5401 tgacattata cggattcgtc gaatacttaa ccgtgttcag gtcggcaatg agctttgcgc
     5461 tgccgatgcc gaatccgagc cttgcacctg ccattgagcg tgattttgaa aatgttcccg
     5521 tgacaagtaa attatcgtat ttctcagtga gcggcactgc gctttgaccg ccaaaatcaa
     5581 tatatgcctc gtctattata acaacgttat cggggttgct ctttacaatt tcttcaatcg
     5641 tttcgagact gagagcaagt cccgtcggcg cattgggatt ggcaattaca acggtttcgt
     5701 ttactgccat atagtcattc gggtcaagtg taaagtcctc tttgagtggg atttccgtat
     5761 aaggtatatg attaagctcg gcaaacacag ggtaaaagcc gtatgtgata tcagcgaagc
     5821 ggagagggtt tttctcgtcg gcaaacgcca taaatgcaaa attcagcacc tcgtccgaac
     5881 cgtttgtcat gattatttca tcgtcattta tgccgaaaac ctcagccgcc ttttttctca
     5941 gggcaacaca ctccggatcg gaataaagct gaagcctgcc tgcttcctcc tcaaccgcct
     6001 ttgcgaccgc ctgcgacggc ggaaacggcg attcgttcgt gttgagcttt acatattttt
     6061 tgtcctgcgg ctgttcaccc ggggtgtacg gaacaagtga agaatattta tcagaaaaaa
     6121 atttgctcat tatttctcga aacgcaccgt tgcgctcttg ccgtgtgcgc tcagtccctc
     6181 cttgtttgca aaatatgata tcttgtccgc caccttttca agagccgcgg aagaatagta
     6241 ggtgaactgc gattttttga caaaatcatc aaccgaaagc ggagaagaaa atcttgccgt
     6301 tccgctcgtc ggcaaagtgt gattcgggcc tgcgaaataa tcgccgagag cctcggggca
     6361 attcttaccc atgaagattg agcctgcgtg ctttatctta tcgagataat caaacggatt
     6421 atcaacacaa agctcaagat gctcgggggc aatttcattt gcaatatcaa tagcaagaag
     6481 caaattatcc tcggcaacaa ttatttttcc gttattgtcg atcgaagctc tcgcaatttc
     6541 ggcacgggga aggagcggaa tctgtgcttc aagttcgtcg cttaccgcct gcgcaaaagc
     6601 cttgtcatcc gttacaagaa ccgcacttgc catcttgtca tgctcggcct gagaaagaag
     6661 atctgcggca acgaatttcg gattgcatgt tgcatcggca acaacaagaa tttcgctcgg
     6721 accggcaatc atgtctattg aaaccttgcc gaaaacctgt ttcttagctt cggcgacaaa
     6781 cgcatttccc gggccgacga ttttatcaac cttcggcaca ctctgagttc cgtaagccag
     6841 agcggcaacc gcctgagctc cgcctacttt gaaaattcgg tcaacgcctg cgattgatgc
     6901 ggcggcaaga ataacggggt taaccttgcc gtcctgcttc ggcggagtta ccataacaat
     6961 ttcggggcag cctgcgatct tagccggaat actgtccatt aaaaccgttg aaggatatgc
     7021 cgccgtgccg cccggaacat aaagtccgac cttttcaata ggcataacct tctgaccgat
     7081 aacaacgccg tccctatcgt tgatcacaaa gcttgttctc acctgctttt cgtggaaggc
     7141 gcggatgttt tgcgcagcct ctgccatgac ggaaataaat tcgggatcaa caaggcttaa
     7201 cgcctcgtca atttcctctt ttgtgacctc aaggctcgca agctcggcct tatcaaactt
     7261 ctttgaataa gcaaaaagag cctcgtctcc gttttttctg acatcggcaa ttatatcggc
     7321 aacaatatcc tcaacacttg cagatatatt ttctctcgca aaaatttcgc tgttcggcac
     7381 ttcgccgtaa ttatatatct taatcatttt caatctgctc ctttatgctt gcaacaatct
     7441 tttcaatctg ctcacgttta aaattatacg ccgacttgtt tgcaatcagc cttgcgctta
     7501 tcggcagaaa ttttgttttn nnnnnnnnna tcaatatctc tgccgatgct gtcgtaataa
     7561 ttcgacgcaa tatttgtaaa ctttgtcgcc accctcagcg tgcgtgtgcc gtcatcattg
     7621 aaattcttcg gtcctgcgac cgccatgtga cattttccta tatcaagatc gagcagctca
     7681 taaacattcg gctcatattc aagcagaata tccttaccgg caacgccgat atccgccgct
     7741 cccctctcaa cataaattga aacatcggag ggctttaccc aaaaatatct gacctgcttt
     7801 tcttcgtttt caaaaatcag tttcctgtta ttttctttta tcgaaggaca ttcaaatcct
     7861 gcggcctcaa acattgcata aaccttttcg ccgagtcttc ctttcggcag agccacatta
     7921 agcatttgaa tccctcctta agtcaacaaa ttctttatat ctcagctttt cgggaacagt
     7981 cttttgtgcg ctaaccgttt tacccgattt tataagctca ttcaccttgt cggcaacggt
     8041 cttgatgccc gttttgccgt cataaacgag aagcacgtca acgtcatagc ccggcttctc
     8101 cgttctgagc tgttcaagca aatcaaggta cagcgcaaat cctatagcgc ctgattttct
     8161 gcccattttt ttcatcattc tgtcatactg accgccggca agaacaccgt cgcatattcc
     8221 gtcaacaaag ccgcggaaaa caaaaccgct gtaataattc atattgttga caacggaaaa
     8281 gtcaaaaaga attttatccg aataaggaga ggtctccagc atttccgata ttgcacgaag
     8341 ctcattcaaa tcctcgtttg aacaaatttt ttcgatctgt gaaataactc tgtttctttc
     8401 gccgctcgtc agaataattg tcgaaagaac ctgagatttg tcatcttcaa tcccgtattc
     8461 cttgcaaagc ttcacaaggt catggctgtt tttctcttca ataagcctga tcgctttctc
     8521 ggaaaatcct gcatcggggc aaacatcatc aagaatcttc tgcaaaaagc cgagatgcga
     8581 aacattcaga acaaagctgt cggaaatc
//
LOCUS       DAWBSW010000080         8482 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1709_length_8482_cov_2.849413, whole genome shotgun sequence.
ACCESSION   DAWBSW010000080 DAWBSW010000000
VERSION     DAWBSW010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_8482_cov_2.849413"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2344 a         2047 c         1861 g         2230 t
ORIGIN      
        1 tataaataat ttgtgaaaag tctattgaca aaatcataaa ataacactaa tatcttttag
       61 tgtataaaat aaattatttt ggaggagttc tatctatgtc agtatcagta ttcgaaagac
      121 tgcaaaagat aatagccgaa cagcttgaaa tcgacgagga tgatatccgc cccgacagcg
      181 acattatcga tgatctcggc gcagacagtc tcgacattgt tgaccttgtt atgagcattg
      241 aggacgagtt ccatgttgaa attcccgacg aggcagttga agaaatgaga acggttgacg
      301 acgcagtaag atatattgag gatcacgaat aataattaaa ggcggtagtt aaattggcac
      361 agcagaaaaa aatggttgaa gaaatcacgt caatggacga agattttgca aaatggtaca
      421 cagacgttgt aaaaaaagcc gagctttgcg aatacacaag cgttaagggc tgcatggtta
      481 tcagacccta cggctatgcg atttgggaga acattcagca cattatggac agtatgttca
      541 aggcaacagg acatgagaat gtatgtatgc ccatgttcat tcccgaaagc ctgcttgaaa
      601 aagaaaagga tcacgttgaa ggcttcgctc ccgaggttgc gtgggtaacc cacggcggca
      661 gcgagaagct tgaggaaaga ctttgcgtca gacccacgtc ggagactctt ttctgtgagc
      721 attacgcaaa catcgttcat tcatacagag atcttcctaa gctctacaat cagtgggtaa
      781 gcgttgttcg ctgggaaaag acaacacgtc ctttcctgcg tacaagagaa ttcctttggc
      841 aggagggtca cacgatccat gcaacggccg aggaggctca ggaggaaaca atccgaatgc
      901 tcaacgtata tgccgagttc tgcgagaaat atcttgcaat gccggttgta aagggcgtta
      961 agactgcaaa agaacgtttc gccggagccg aggatacata caccatcgaa gctctcatgc
     1021 atgacggcaa ggctcttcag agcggcacat cgcactattt cggcgacggc tttgcaaagg
     1081 cgtttgacat tcagtttaca ggtaaggata acaagcttca tcatcctttc cagacctcat
     1141 ggggaacaac aacacgtctt atcggtgcta tcattatgac acacggtgat gacaacggac
     1201 ttgttcttcc gccggcggtc gctccgattc aacttgttat cattcccatc gcctcccata
     1261 aggagggtgt tctcgataag gctaacgagc tgaagaataa gctcgcaagc gtttgccgag
     1321 ttaagcttga cgactccgac aacagcgccg gctggaagtt ttcggaatat gagatgaagg
     1381 gtgttccgct tcgtcttgaa atcggtccca aggatatcga aaacaaccgc tgtgttgttg
     1441 ttcgtcgtga taacggtgag aaaatttttg tttcacttga tgagcttgaa gcaaagatcc
     1501 ccgaattgct tgaggctgtc cgtgacggtc tttatcagaa ggctattgac cgaagagcgg
     1561 caatgacctt caccgcaagg gactatgcag agctcaaaga tattgcagac aacaagcccg
     1621 gttttatcaa agccatgtgg tgcggcgacc gtgagtgcga ggataagctc aaagacgagc
     1681 ttggaataac atcacgttgt attccgttcg agcaggagca cctcagcgat acctgtgttt
     1741 gttgcggcaa gcctgcaaag cacatgctct attggggcaa ggcatattga ttcagcgata
     1801 atattaaaga cattcccgaa aaaggaatgt cttttttata taattctatg tattactttt
     1861 ttgtttgctg ggtcggtccg taatttgccg ctccggtata tgcaacatcc tccggtgtcg
     1921 gatagtattt cggcttggag agatcacgct gataaagtct gacatgctcc cctctcggca
     1981 tcaaatcaac gacatcacat ccgcgccaat aaaccgtaaa ctcaattccg cgcaccgttt
     2041 ctttcttcgc aacagtctcg atatcaaacg taagccggag aacgtccccc tctttcattc
     2101 ccgcaaatgt gccctcgatt tcttcgccgt tgagagtaac cgatatatca cgcatccaat
     2161 cgtacctgcg gaagcctgct ttgcacgcct tttttgccgt aaggcaaatt atgccatcat
     2221 tagaaataaa tgattccatt ttaagctcgg cggtttcctt gtcgcaggga atattgaccg
     2281 tcagaacccc gtcattctcc gttatcgcat tgtcccacac ggttttcagc gccacgggag
     2341 ccgtgccgac gcaacagccg gcaatcgaac gaaaacgtga aagcgaaatc gaattaggct
     2401 cacttccgcc ggtaaatccg ccgatcatac gtttgtcgat gtcacggtaa gttattccca
     2461 agccgtcggg tcttgaatta tcgcactcga cgtagcccgt gtatttaacc tgattttcca
     2521 taagctgatt gcgtgcaaag agattcatat cattccaata ctcctcataa ccgcacttga
     2581 taagctcatc ggcacagact atcatatccc tgatacagca agtttcacag tgttcctcct
     2641 caacgggatg ccactgcgca tactcgggca cccagccaaa gtccgaagaa agcgaacgta
     2701 cataatcgta aattgctttg ccgtctttaa tatatctttc ctcgtttgtg agtctgccga
     2761 gcttcacaac tccgcacgca aaccacattg ccgaatgaac atgtccgaaa aactcctttt
     2821 tatagttgaa atatcttgag gaatcgagga cataattagc aatacctctt gcaaggtcaa
     2881 gcgcaacctc gtctcccttt aactcgtacc ggcggacaag cgcctgtaaa gaaacggcat
     2941 tgcgaatcac ctgctctccc ctgcccgtat gccgggtaag gtcaaatccg ccttctttta
     3001 aataaacatc gttaggaaat tcgtaaagcg gctcgtcctc gtcataatca cctgaccaga
     3061 aggtacacac cttgcgctcg atcacaagtg aacgcatacc gcgcacaagc tcggatgcac
     3121 gtttttccgc ctcctcatcc tccggatatt cctcggtaat catattaagc gcaggcagaa
     3181 tatatcccat ttcgtgaaaa gacgcatgaa ccgtgtgcgt ccacggatat ttctcgcaga
     3241 agcgcagtcc gtgctcgccc catgatttca taagatgacg gcgcagtgac tgcttaacat
     3301 catcaccctc gtttgtgtta agcataagca ttgcgcttgt caagccctca taccatgatc
     3361 cgacaagctc ggcatcgtca acacggcaat gcgccgcttc ggcaggcttt ttatttggga
     3421 gaagcaacca atatggcagg ttgtcataag ccttgtcgca catattcgtt atgtaatgat
     3481 ggaccagttt tgcactgttt atcgggtcaa atttctcata cattgcaatc acctcatgaa
     3541 aatttcattc tgttttgcgg atttataaat attattctat cataatatcg gcaattgttc
     3601 aatcacttgt tgataaagct tttttaaaac tcaaaaatgt tgtccgtata tataatttta
     3661 ttgactgaaa agcaaaacga tattataata ttcaaaaagc ttatcaaagg aaagattcca
     3721 tgaaaatacc aaaaaggctg tctgcaattt tgctcgctgc ggcaattata atgctgactt
     3781 cttgtcagac aaaaaggaca tattcaaatg atattacgac actttccgat caggcaagct
     3841 ccacgactgt tgttgatata acgaaagaga cggccgtgca agccgcaacc gttccggcaa
     3901 ctgccggctt aaccgcagta tctgcaacgg ccgcagccac gacaaaaacg cctgctgacc
     3961 cgtacaagac agcactcaca tactgcgttg acacggacag gttcagtcag caaaaaaaca
     4021 ttgacaagcg catttatcct gcgagcatga cgaaattcct gaccgcttgc acggcactca
     4081 aatacgtcaa gcccgatgca aaattcaccg tcggttcgga gcaggacatg gttcagcccg
     4141 aatccagcct gtgccttatt caaaagggca acgtgctgac tctttacgac ctgttgacag
     4201 gacttttgct gatgtcgggc aacgatgcgg cttacaccat agctgtcggc acggcacgtc
     4261 agcttcgtcc gaacgataag ctcagcgaca agcaggcgat cgcatatttc tgcgagctga
     4321 tgaatcaaac cgctaaagaa atcggcatga cggacagccg gtttgaaacg cccgacggct
     4381 gggacagcaa gaatcagtac accaccacgg cggatcttct gactctgaca aaatatgcaa
     4441 ttaaaaatcc aaacattaag cagataattt ccattaaaga gacgaaagta acctttgccg
     4501 caggcggaag cataacgtgg aaaaatcata ttaagctcat tgacccgaaa agcccctact
     4561 attgcaagga ctttgtcggc ggcaaaacag gtacaaccga cagtgcaggc tattgcttga
     4621 tatcggtttt cgagaagaac agcaaagaat atataatcat tgtaacagga tgcaaggacg
     4681 ataattcacg ctgtgccgct acgcttgatt tgtacggacg gattaagtga tcgtttaatc
     4741 actgcagcaa taaaaacaca gcagcggttt tctccggatt tacacggtgc gatgagactc
     4801 attaaagtaa tattttgtag aggccgatgc ccacatcgac cgaagtacag cagaaatttt
     4861 attaaaacaa ttataaaaag gctatgacag gtttttatcc cgtcatagcc tttgcttatt
     4921 caattttaaa cagtctgcga gcattttcgt ttgcggtatc tataagcttt tgcgtgtccg
     4981 tgtgtctgac ctcggcaagc ttttcggcgg aatatgcaat caaatccgac gaattacgcc
     5041 ggcctctgaa cggcacaggc gccatatacg gacagtccgt ttccagcaac agcctatcct
     5101 cgggaatgat ttcggcaacc tcaagcggtt tgcgtgcatt cttgaacgtg actgctccgc
     5161 cgagaccgat atacattccg agcttcagca cctcttttgc catctcggcg ctgccggaga
     5221 agcaatgcac aacgcccttt ggcttgtacc ttttcaagag gttcattgtg tcctcatgcg
     5281 cttcacggtc atgaacgata acgggcagtt caagctcgtt tgcaagaata atctgctttt
     5341 caaaaatatc aagctgttta tccttcggaa caaaatcgta gtgatactcc aatccgattt
     5401 caccgacggc aacgcacttc ggatgcgaat aaagctctct gattttttga agctcatcat
     5461 atgaatcgcc cgtgatgttg ctcggatgaa ctcctacggc agaataaaaa taatcatatt
     5521 cctctgagaa ttcaatgctt ttaaggctcg atgcaaggtc acagccgcag ttgattatat
     5581 ttacaacacc ctttgagggc agagatttca aaagatcttc tctgtcctca ttgaatttct
     5641 catcatcata gtgggcgtgc gtatcaaaaa tcgagtggta cattatctta tctttgctcc
     5701 gttcggcatg ccgtcaacaa atacgacttt tgcctcgtcg cctgcgtcgg cggcaagaat
     5761 cattccgttg ctctcaacac cgcagagtac ggcaggcttg aggttggaaa caacgattac
     5821 cttgtgtccg ataagctcct ctggcttgta ccattttgca attccgctcg caacggttct
     5881 cggcgtcttg ctgccatcgt caagcgtaag ctttaaaagc ttcttcgctt tcttgacagg
     5941 ctcgcagtcc ttgatttccg caaccttaag ctcaacctta gaaaaatctt caataccaat
     6001 ctttgcaagg ccctctgctt cctcgacctc ggcattttcc tccttggctg cctcttgagc
     6061 agctttggct ctcgcttcaa tcttctctaa catttccttt gcgtcaattc gtgcaaagag
     6121 cggctcggcc ttacctacaa cagtgcctgc cttgagtgca ccaaaagctg aaagcgaatc
     6181 gtaatccttg atatccgtgt taatttgagc gagaatcttc tcagatgttt cgggcaggaa
     6241 cggagaaaga agaacagcta caaagcgaat gctttcaagc aaattgtaaa gcacggtgcc
     6301 gagacgggct ttcttactct catcctttgc aagtgcccac ggggctgtct cgtcgatgta
     6361 tttgttgcag cgctttgcaa gcgtgaatat tgcgtcaagc gtatcgctga ccctgtattc
     6421 cgaaagaagc ttgtccacct tgccgacagt ctcaaccgca agagctttca actcaccgtc
     6481 aagctcctcg gttacgtcgc ccggctgaac aacgccgtca aaatacttat tcgtcatggc
     6541 gatagttctg ttaacgagat tgccgagtgt gtttgcaagg tcggcattgt agcgttcgat
     6601 catgttttcg tatgtgattg aaccgtccga agcatagggc atttcggcaa gcaaatagta
     6661 tcttacagca tcaacgccga acaggtcaac aagatcgtcg gcataaataa cattgcctct
     6721 tgattttgac attttgtcgg tgccgaaaag aagccacggg tgtccgaaaa cctgcttagg
     6781 aagcggctct ccgagagcca taagcatgat cggccaataa atagtatgga aacggacaat
     6841 atccttaccg attatatgaa cgtcggcagg ccagtatttc ttatactgct cactcgagcc
     6901 gtcgggatcg tagccgatac ccgtaatgta gtttgcaaga gcgtcaagcc aaacgtaaac
     6961 aacgtgcttc gggtcaaaat caacaggaat gccccattta aacgaggttc ttgaaacaca
     7021 aagatcctgt aaacccggct tgatgaagtt attaagcatc tctttctttc ggctttcggg
     7081 atagataaag ttctcgttag tctcaatgta ttcctctaac tgtttctgat attttgaaag
     7141 cttgaggaaa tacgcctctt cacgggcagg cttaacctca cggccgcagt cagggcactt
     7201 gccgtcaatg agctgtgatt ctgtccagaa ggactcgcag ggggtgcagt acattccctc
     7261 atagtgaccc ttatagatat caccctgatc gtagagcttt ttaaaaatct tctgaataac
     7321 tctctcgtgg tcggggtcgg tcgttctgat gaacttatcg taggtagtgt tcaaaaggtc
     7381 gcagatttct ctgatctcac ctgcaacacg gtcaacatgc tgcttcgggg tgataccctc
     7441 tgcggcggcg atctcctcaa tcttctgacc gtgctcgtcc gtgccggtct ggaagaaaac
     7501 atcgtagccc tgtaatcgct tgtatcttgc aattgcgtct gtcattacga tctcatagct
     7561 gttgccgata tgcggcttgc gtgaggtata ggcaatagcc gtcgtaatat aaaactttcc
     7621 tttactcatt atatatcctc ctctgtttat tattgtcata ataaaaatgt tttaataaat
     7681 ttcagccgta ccgttatcgt ttagatagct tttcgtttag cgtttccgac ggaaaaatag
     7741 ccaacgagag caaaaaaata agtctcaaag cataatactt tgagacgaga aaacccgcgg
     7801 taccactcaa tttgtaatcc ttatcggaat accactcgat tctttaacgc agatatacgg
     7861 aagaccctac tgttatttca ggccttcggc tcaaaagcca taggcacatg gagcttcact
     7921 gtcggctctc acctgccgcc gactctctga aaagctttgc tccggccctc tttatcacag
     7981 ccgttgcaag aataataaca ccaatttgaa aaaaagtcaa ggtcagataa tctcagattc
     8041 ggcaatatcg ggactgtttt tctttgctat tcttgattta atgcacggca caagcgttcc
     8101 gactattgtg cagacaaccc actcgatata tgcattatac gttatcagtg ttctgacaat
     8161 ttgagtaggg tttgagccga ggttctgtat atagtcgctg ttgtaaaatg caataagcaa
     8221 tgccgcaaca aagaaaacag tgttgaggaa tccgccgctt aacgacgaaa taatgttcgc
     8281 aaatatcggc gaggtgtttc gactgaaaag cttatgtata agtccgcagt taagacccat
     8341 cagcgctctc ggaataatgc agacaagcaa taacagaatc ggactgatgt gcagaaatgt
     8401 ttgtccgaaa gcatagttcg gatcaaagca atgaacaaag ctcgaaattc cgaaaaccgt
     8461 gccaagataa agtccgcaaa gc
//
LOCUS       DAWBSW010000081         8425 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1726_length_8425_cov_2.947551, whole genome shotgun sequence.
ACCESSION   DAWBSW010000081 DAWBSW010000000
VERSION     DAWBSW010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_8425_cov_2.947551"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    928..937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4605..4614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2114 a         1943 c         2007 g         2341 t
ORIGIN      
        1 tttgatttga gccaaatcca agaataatct gcaaatttca tatactaaga acttggtgga
       61 cacctattcc tatgcgcatg aaagacggta gtacagttgc actttttaac gaaaggcaca
      121 ccttttaacg atttatacga ggggtgtgca tttggtatca gaattggtca ctactttcaa
      181 ataaagaaga gtcgcagcag cggctctttt ttattttacg cgggacttga acccgagagg
      241 gtctgagcgt taaaaaacag tcctgtggac tgtttttagc gaggagcggt gagccgggta
      301 ctgtcgctaa gcgacggtcg gcgagccatc agcatgcaag gcgcagccgc agcagacgcc
      361 aagttcccgt ctctcgcacc aaataaagaa gagtcgcaac agcggctctt ttttatttta
      421 cgcggggctt gaacccgaga gtgtacgagg gttaagctat ggtgtgttat tcgtatcatc
      481 acagggtatt ttactgccat tttaaactac aaaataatct aaatgtataa atcggtaaaa
      541 aagttcataa ttggttattt tttacgttat atattcataa tatcatggct ttaacatcat
      601 aataatctta ttatacagga ttattattgc ggaggagtta aggttatgaa agaagttaaa
      661 caacccaaaa aaccgattat ttattactac ggaatagttt tactgattat tctcattttc
      721 aacatggttg ttgcaccgct tattacaaat acaagggtga aagaagtgga ctacggtacg
      781 ttcatgaaga tgattgagga gaaaagtatc ggaagcgttg aagtcaatga ttccgaaatc
      841 attttcacgg ataaggatgc aaagcagatt tacaaaaccg gagaaatgaa tgacccgaac
      901 ctgaccgacc gtctttatga gtcgggannn nnnnnnngag ctaaattcac ccgaaacatt
      961 gaaaaaggaa tgtcgccgct tttgagtttc ttcctttcgt ttattttgcc gattctgatt
     1021 tttgccgcac ttggtcagta tgtaggtaaa aaaatgatgt cacaggcagg cggaccgaat
     1081 gcaatgtcct tcggactggg caagagtaac gcaaaggtct atgttccgtc gagcgacgga
     1141 atacggtttg ccgatgttgc aggtgaggac gaggcgaaag agaacctgtc agaaattgtc
     1201 gattatttgc acaatcccaa gaaatattcc gatgtaggtg catcaatgcc gaagggtgtt
     1261 ttgcttgtag gccctccggg cacaggtaaa accatgctcg caaaagccgt agccggtgag
     1321 gcaggcgtgc ctttcttctc aatgtcgggt tcggaatttg ttgagatgtt tgtcggcatg
     1381 ggagcttcaa aagttcgtga tcttttcaag caggcaaaag aaaaggctcc gtgtatcgtt
     1441 tttattgatg aaatagatgc tataggcaaa aagcgtgacg gacaaatcgg cggcaatgac
     1501 gagcgtgagc agacgctcaa ccagcttttg acagagatgg acggatttga cggaaataac
     1561 ggagttatta tccttgccgc aaccaaccgt cctgaatcgc ttgatccggc actgacacgc
     1621 ccgggaagat ttgacagacg ggttcccgtt gaacttcccg accttgcagg acgtgaggca
     1681 attcttaagg ttcatgcaaa aaaaatcaag acagccgata acgttgactt tcataccatt
     1741 gcccgtatgg cttccggtac atcgggtgcc gagcttgcaa atataatcaa tgaagcggct
     1801 ttgcgcgcgg ttcgcaacgg acgaacagtt gttgaacagg ccgaccttga ggaaagcatc
     1861 gaggttgtta tcgcaggata tcagaagaaa aatgcgatta tgacagatga agaaaagaag
     1921 atagttgctt atcacgaaat aggccatgca cttgttgcgg cgttgcagac ggcatctgcg
     1981 cccgtgcaga aaattacaat aattccccgt acatccggcg ctctcggcta cactatgcag
     2041 gttgagcaga acgataaggt actgatgacg aaggaagagc ttgaaaataa aattgcaacg
     2101 cttacgggag gacgtgcggc tgaagaaatc gtgttcggac aaattacaac cggagcttca
     2161 aacgatattg agcaggcgac aaagctggct cgcgctatga ttacacgcta cggaatgacg
     2221 gatgattttg atatggttgc catggaaacg gtcagcaatc agtatctcgg cggcgatatg
     2281 tcgcttgcat gctcagccga gacgcagaaa gagatcgata aaaagattgt tgatacggtc
     2341 aaaaagcagc atgaaaaggc acgcaggcta cttgcggata atcgggagaa gcttgatgag
     2401 ctcgcaaagt atctttatga gaaggagaca atcacaggca gtgaatttat gacaatattg
     2461 agcggaaagg acggtgccgc ggtatgaaca aaaaatcaaa cttcggttgg ggcgaactga
     2521 ttataggact gcttatgacc gtgctcggaa ttttcacatt cattaaaccc gaaaacattt
     2581 tatcgggcgc aattattgct tacggaatta ttatagccgt gatgggaatt gatgacatta
     2641 ttgtttatgc gagggtgtca cgtttcacgg gattcggacc gacgcttgcg ttgatttccg
     2701 gtattctcag tgttatgtgc ggcattatgc tcatcgctaa tccgaacata ggcaaatggg
     2761 cgctgacgat tcttatgccg atttggttta ttgcacacag tatcgcagga cttacgaggt
     2821 caaacttcat tcgtttggtc ggcaatgcag tttattatta tctctcattg atattgaatg
     2881 ttctgggtct gatcatcggt attattatga ttttcagtcc tgcaatgtca tttttaactc
     2941 tcagagtagg cacatatatc gtatcccggc ttctccttgt tttaggaatt gagaacatca
     3001 tttccgcttt tgcaagacgg aactacgatt ggtgaaagcg agaaaaagag tactgttaaa
     3061 attatataag gctgtgacgg aacatacgtt ttgtcacagc ctttattgat attatttata
     3121 taagtctaaa ggttttaacc tcatgcggtt tcatggtgaa ctcgaattca ccgttagtgc
     3181 aaggaatttc gtgctcgtca cactccataa ggttacattc gataaccttt gaatatccga
     3241 ggtcgaacca cgctttcgcc gtgcagtgac gtgtctcgga ttcgtacatt ctgacgatta
     3301 ctccgttgcc gtcctgagcg ggcttgactg catcaagaat aacgctgtca ccttcaattc
     3361 tgacaaatgt gtggccgata tcactgccgc cgtttgcctt tataaactcg ccgtcaagtc
     3421 tgatgtttcc ctcaaagccg agccttacgg tatcggcgtc acgccatgtt ccggtgtgcg
     3481 gatagaagga atatctgaac gagtgcattc cgatatcgcc gacaggatcg gggaggatcg
     3541 gacctctgag aagcgttatt ttcatcacgt tgtcatgcac gttgtagccg tacttgcagt
     3601 cattgatgat gcttgcaccg tagccgccct cagagagatc ggcccacttg tgagcacaga
     3661 actcaaactt tgcctgatcg tagcttgtgt tgtaatgggt cggatattcc gccgcaccgt
     3721 gagcgatctc acagcttgcg ctgcggcttc tgatgtcaac ggggaactct gccttgagca
     3781 ccttttcacg ctcgtgccag tcaactgtcg tgtcaaaatc aaccgtcggt ttatcctttt
     3841 caagaatgat atcctgagtt attgttgact catggaactt tctgacaaca cggataatgc
     3901 ccctgagcgg tgatgcctca acaacctcaa tactctcggc tttgtcgagc ggccagaact
     3961 tcatctgata gtcaaactcc atgttccatg cgctctcgtg aatgggcttg tcgtgagaaa
     4021 ttgtgagcag gttgcccttg cccgtgagaa tctgtctgcc tgtctgcttg tcgattacct
     4081 cgtcgagcag gccgttatcg tcaagagtaa tcttgagata tttattttca agtgaatttt
     4141 tcgttgcctt gactgtatca aactccgccg tttcatcgca gagagtgaaa actccgtagc
     4201 cgaatgcagg aacgggatcg gcgtaaaact cgatataatg cttgtcgtcc tcgtcgtaaa
     4261 ccttgcttgc aaggaacttg ccgtcggaat ttttgatgcc ctttgccgca tacggaatct
     4321 ctgccttgac aattcccttt gtctgccagg tgagcatatt ccaaacaaga accgaatcgt
     4381 ccttaacgct cacattgttg aggattttat tcttgccgtc acggacaagt ctgccaccaa
     4441 gctcacgaag ctcggcatat tcttctctcg tgttctctat tgcctcatga atcgaggtgc
     4501 cggggagaat atcgtggaac tgattgatga gcaggatctt ccatgccttt tccatatcct
     4561 cggcaggata atctccgccg aaaacgttga gaatttcggc atttnnnnnn nnnnttctca
     4621 tcataaattc gccacggcgg ttgttcttct tgatgaaagc ctgactggtg aaagtaccgc
     4681 ggtggttttc gaagtaaagc tcgccgtcgt aaacaggaag cttgtcaaaa tcgccgtcaa
     4741 tagcgtggaa gaactccgaa accttgccgt ttctcgtctt aggcataccg gggagctttt
     4801 caagtcgttt gcctcgttca acctgattaa aggtgcatcc gccgccgccg tcgccgtagc
     4861 cgaacatacc gcaggatacg tcaacaatgc tgttctgacg gttgtttttg cgaagtgtgg
     4921 ttatgtattc tgcgtcgtat tcactctggt acggcttttt gcaaagataa gcgaggattt
     4981 cgtctccgct gtgcgctctc caacggaaaa cgctgtacgg gaactcgttt gtgtcgttat
     5041 agaaaagctt tgaggtcatg aaatacttca ttcccgaacg cttgattatc tgcggcagtg
     5101 cccatgtgaa gccaaagcaa tcgggcagcc agtaaatatc ggacgagaca ccgaattcct
     5161 tcataaagaa ctctctgccg taaagaagct gacgaatgag agcctcgccg cttgcgatat
     5221 ttgtatcggc ctcaacccaa gcgttgccga cgatttccca ctgaccgttc ttgacctttt
     5281 ctttgacgcg ctcgaaaatg tccggatagt gcttcttcat gaagtcatag agaaccgcct
     5341 gactctgagt gaacttgaag tcgggatagt tgtccatgag tgcaagattg ttagagaaag
     5401 ttcttgccgt ttttctcgta atctctttga ctgtccaaag ccatgcaacg tcaaggtggc
     5461 tgtgacctgc cataataatt tcgccgggtg aggagtaatt gattttttca atctcgcccc
     5521 agagaacatt gactgctttc ggcacggaag cgataaacgc ctcgtcgccg aggtcataat
     5581 caagctgatg aaccgaattg tccatagcgt tgaaaaggcg ggatttaacg tattcgtcct
     5641 cgcagtgctc gtatgcgtcc caggcggtcg agatatcaaa ccaatagctc tccgtcagcc
     5701 tgtcaacagc gataagctcc gccttgttga gcgtgtattc catatatttt gcacctgcaa
     5761 tcgcacggtc gcagaagcct gcacagttga ccgcaccctc aacctgaatg ttgagctttt
     5821 cgccggcttt aactccgtct ttgaaaagaa tttcagtgcg atgaacccag ccgaagttct
     5881 cctccgagct gactgctccg acgatttgac cgttgattct gacaatggct tcgccgccga
     5941 aatcaatcga aagataaacc tttttgccct caaattcggc aggaacggtg atctctgatt
     6001 caaaatatct cgtctcgtcg tagccgacct tccatctgtc gccgagatac atctgaatgg
     6061 gcggattctc gtcaagaaca atgttgttcg ggctgacgtg catgcccgtg tagcttttcc
     6121 acgagtcgat ttcaaggcga tttgtcacgc agtacggctc aatctgcctt atctggtttt
     6181 taagtacgtt cgggttgagt gtgtgcattt ttttgaccat tcctttcttt ggaggaagtt
     6241 gaaattatat aattctgaaa ttatgtgtta tattaaaaat gtttgcttat atcgtgttca
     6301 gcagaaatct tacctgtaac actcaacaat acctgcattg ccgctgagct tgaatctgcc
     6361 ctcggcggca acgggcatca ggaagtaatc gccctttttg attgcctttt cgtagccgtc
     6421 acccgtgatt ttgccctcgc cctctgtgac aacatagatc cttgcgccgt tgtcaaggct
     6481 gaattcgccg cccgaaagct cgattttcct gaccgtgaag ctcgttgtca ttctctcgtc
     6541 aatgaacgaa aggtacttta caccgccctt gtcactgatt gttacaggct caagcggagc
     6601 gggatatttt ttcgcaatgt caaagcattc catggccgta tccttgtcga gaccgaggta
     6661 catctcgttg tcgctgagct tgtaatcacc gcaccagcgc tcgggctgaa ccgtgaaatc
     6721 cgacggctcc tgaacctcga gaagcagaca gcctgcaccg attgcgtgaa ccattttcgc
     6781 aggtatatag ataacgtcgc cgggcttgac ctcaaaggat tcgagcagat cctccataac
     6841 ggttttgctg ttttcactct cggcgatcgc cttttcaaac tgctccatcg tcacgccgtc
     6901 cttgaagccg tagaaaatct ttccgtccgg acgtgtggca aggatatacc agctctcctc
     6961 cttgccgtat gaggagttga aatactttct tgaaaaagcc ttgtcggggt gcgcctgaac
     7021 ggggagccga atcgcactgt caaggatttt tgtcagcacg ccgaggtcgt cacgctcgcc
     7081 gataatttcc tttttatatt tgtcaagggc atcattgaga taaagatcct cgcccttgat
     7141 tttagagata ccctcatatt tgtccgtgct tccctcattg agagcatgaa cgcttgaaac
     7201 aacccattct tcgggatagt tgccgtcctc ggaatcgtcc gagaagaagt cggagaagag
     7261 ctttcctccc ttgtagacac ggaaaactct gttctgctcg aagaagatcg gataattgta
     7321 aaacatattt taaccccctt ggttattcgt acctgataag ctgtcttgcc ttatcaagac
     7381 tctgataaat tccgctgcct gccattgccg aaagtgccgc accgtaagcg gcttcctcct
     7441 tatagagcgg aatactgatt tttttgccga acatctcggc agtcacacgt ctaagcgccg
     7501 gatttttccg tattccgttg cctgagcaga caattttttt gcagtccttg ccgatgagct
     7561 tatacatatc gtgaagctcc cgagacattc cgataatcgt cgaaagcaag atatcctcgg
     7621 gtgtaaaatt attctcgtca atgcccgagt atgagccttt gagtgtcgga tcaagccttg
     7681 ttccgcaaaa tcttgtgtcg gcaatgattg aagttgcgct cttttttttg agagccttat
     7741 caatcctttt gtatatattt tcgcattttt cgcccgttgc aagctccgca cattttgcaa
     7801 tgaagttcgc cgcagtcgca aaggctctgc cgccgcagag agcacaaccg gcatagagat
     7861 acttgccatc ggtaaacgga cgtgcttcta taccgtcagg tgcgtctgaa ctgttgccga
     7921 tgaccgatat ctgcgagccc gtgccgaaat tgagcaatgc actttcattg tcgctgaccg
     7981 agccgataaa gctcgcttga ttgtcaccga gagcgacagt gacggggatt ccggcatatt
     8041 cgccgactgt tccaatcttg tctgttactt tcggcagaca gctcaaatcc gtcttgaatt
     8101 tattattttt aatgtcgaac aagccgagcg aagcggcatt tgtaacgtgc atgatcggct
     8161 cggatttttt gcaaagagtt attgcaatgt aatcgccgat tgtcattatg tattttgtct
     8221 tttcgggcac aagtccgttc tcacggttat agaagtcgga gaccaaaccg tagcctgcaa
     8281 atgagccgag acgctcggca tagcttttgc cgtccttgta ttcaaggttg cccctggcgt
     8341 cctgccaggt gtaaagcgga gaaatcgcct tgccgctctc gtcggcgtag agaataccgt
     8401 tcatcttatt ggaaatgccg attgc
//
LOCUS       DAWBSW010000082         8351 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1738_length_8351_cov_2.370540, whole genome shotgun sequence.
ACCESSION   DAWBSW010000082 DAWBSW010000000
VERSION     DAWBSW010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_8351_cov_2.370540"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    657..666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1763..1772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2339..2348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2968..2977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4247..4256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7894..7903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2169 a         1975 c         1653 g         2494 t
ORIGIN      
        1 ttttcgggcg tctgagaaac aatctcattc ggcgtaacgt cgggatcgac cttttctgtt
       61 acttcaaaaa cataattggg atatttgttt ttaaattctt cctcatcata aattattccg
      121 acaaaattcg gaaccttgac cttagagttg cccgtaagat aaccggtctt aaagagaatg
      181 ccgaaagcaa cggcgagcac aacaatgagc gccgtaataa taccaacaag aataggaata
      241 gtcctgttct ttacaacggg ttcttcctcc tcctcatctt cggttacaac agggtcaata
      301 tgtttaattg atcccgtgat attcggaata tactttgtcg gctcggtatt tttgtagtca
      361 tagtcaaact gaattgacgg atttcttctg aactcctcaa ggtcaagaag catctcggcg
      421 gcagactgat atctgtcgtt gacgttcttc tgcatcgcct tgagaacgat ctgctcaaga
      481 ccgacgggaa ttgaaggatt gatctctctc ggcttcacgg gatcctgctg gagctgcata
      541 atggcaacgg aaacagtgtt atcagactca aacggaagct tgcccgtgag catttcataa
      601 agcataacac cgacagaata gatatccgcc ttgtcatcgg taatgtcgcc tctcgcnnnn
      661 nnnnnngggc ttatgtaatg aaccgagccg atcgtcgagt ctgtcatcgt tctcgtttca
      721 ctgcgagaaa atcttgcgat gccgaaatcg gtaactttaa ttgcgccgtt ttcaagaagc
      781 ataatattct gcggctttat atcgcgatga acaataccct tatcgtgcgc atgctggagt
      841 gcacggagga tctgcgttac aaagaaaaca gcctccttat aattgatcac accctgctgc
      901 tgaatgtatt ccttgagagt gattccgtca acgcattcca tgacgatata ttgaattctg
      961 tcgccgtagc tgacatcgta taccttaaca atgttcggat gcgagagcac ggcaatagcc
     1021 tttgattcgt ttttgaatcg gcgtctgaat tcctcattgg caaggaattc ctctttaaga
     1081 attttaatgg caacagttct gtcgtcgata ttgtcatacg ccttataaac aaccgccatt
     1141 ccgcctacac cgattatttc ctgaatttca tagcgaccgt caagtttttt tcctacataa
     1201 ttttccataa acagagtccc ttcttaatta ctcacttacg gtgacaactg taatattgtc
     1261 accgccgccg ttttcatttg caagttcaac aagcttatcg gcaattttat atctgtcctc
     1321 ggttttacaa acctcaagaa taacttcatc gtcaacgtag tttgtcaatc cgtccgtaca
     1381 aagcagcact aaatcatcct cgttaagctg ctcctgggaa taatctattc taacctcggg
     1441 gtcaacaccc aaggctcttg taattacatt cttcttaggg tgctcctttg cctcttccgg
     1501 cgttatttct ccgttatcaa ccatcatttg aacaaccgaa tggtcagttg taacctgagt
     1561 tatgcccgaa tcggaaacaa cataaattct cgaatcgccg acgttggaaa tatagagcgt
     1621 atcggtgttt cttactgcaa gaacaaccgt cgtacccatt ccgctaagat cctcgttatt
     1681 gtttgccatg ctgtagaggg tgatgttagc agcatcaacg gaagatataa gcagatgttt
     1741 tgccgaattg tcgttcatgc cgnnnnnnnn nntatgaatc ctgattgtta gagcgaacct
     1801 ttcctacatc ggtttttgcg gaaattttca tttgtttccc tccttttcgg agatttctct
     1861 gttatgacgg cttctgagct gtccgcagga agcgtcaata tccgaaccga ggctgcgcct
     1921 gactgtcgca tttatgcccc gtgaatttaa tattttaata aagctttcaa gtctttcatt
     1981 tgtacttcgc ttgtgcgagc tttcgactat ctcattcgca ggaatcagat ttacatggca
     2041 caacattcct tttaaaagag ctgccagttc ataagcgcag gcgtcgctgt cgttaacgtc
     2101 cttaatcata gcatattcaa atgaaattct tcttgaagtc ctctttgtat attcgcggca
     2161 tgccttcaaa agctcctcga tagggtactt tttgttgata ggcattgtct ttgaacgcat
     2221 ttcattgttg ggagcgtgca acgaaaccga gagcgtaagc tgaagatgtc tttccatcaa
     2281 atcatatatt ctcggcacaa ctccgcaggt ggaaagcgaa atatttctaa gtgataaann
     2341 nnnnnnnncc gtcaccgaca agctctaaaa aacgcattgc gttgtcaaaa ttatcgagcg
     2401 gctcgcccat tcccatcatt acgacgtgtg aaacacggat attcatgtct ctttgcgccg
     2461 catggatctg tcctatcatc tcggacggaa aaagacttct gacgcaaccg ctttttgtcg
     2521 atgcgcagaa aacgcagccc attttgcagc cgacctgtga cgaaatacaa agcgtgtagc
     2581 cgtatttgta tttcatcaaa acagactcaa taaaattgcc gtcgcaaagt ctaaataaat
     2641 atttaaccgt tccgtcaatt ttcgagacct gcttcaattc gatctcacaa taccgtatcg
     2701 ggtatttttc tctcaacgct tcccggagct gctttgaaat attcgtcatc tcggaatagt
     2761 ccattgctcc gtgaacctga agccattgat aaatttgacc tgccctgaat tcaggctgac
     2821 cgagctcacg cattaccgac tttatctcgg gcaaggtcat tgatttaatg tcgtattcgg
     2881 acaaataaac acatcctcac ttaactcttt caacgcagga aataaaaaat ccgtcacagc
     2941 cgtgaatatg cggcataagt gtaagatnnn nnnnnnntca tatcttttca tctccggcaa
     3001 aacacaaacg ctcttaaagt catcgtgttc cgacaggaac ttattgataa ttttctcatt
     3061 ttcctcgggg ttgagagagc aggtcgaata gaccaatttt ccgccgacat taagatatcg
     3121 gcttgaaaca cacagtatag aatactgcaa atccttcaat ttgtcaactt cctcactttt
     3181 cttgaacctt atctcaggct ttttgcgtat aacgccgaga cccgaacagg gtacatcaca
     3241 tagaatctta tcagcctgca aaagtgaaga attgaacttt gaaccatcgt tttgaattgt
     3301 ttcaatatta tttaaaccga gtctttcggc tccgtcattt ataagcttta atcgctgcgg
     3361 atatatatcg catgaaatca ttttgccggt gttgtgcatc atttcagaaa gcgtgaaagt
     3421 ctttccgccg ggtgcggcac aaatatctat aaccgtttct ccgtcacgga catcgagagc
     3481 cttacagcat agctgagacg ccgtgtcctg aacatggaac aaaccgttcg aatatgcctt
     3541 ggttttatga acggcaccgc cgcttataac agtcagcgag ttttcggcca agcggttttt
     3601 ctcacattga aagccgtcgg ctaaaagaat ttcacaaagc tcctcggcat ttgtttttgt
     3661 tgtattaact ctaattgtgt tttcaacagc tccgaacgac gcttcaagaa ttttttcggc
     3721 attttctctg ccgtagcttt cgctccacat tttgcaaagc cattctggag cggaatactt
     3781 aacactcata ctgccgtctg ttttaaagcc gtttttgaca attttatgta agacggcatt
     3841 gacaagtccg gaggcaaaag ctgccccgtt cttttttaca agcttaacac tctcgttaac
     3901 ggctgcggaa tcaggaattt tgtccataaa caaaatctga tatgcgccga gcctgagtgc
     3961 agtcaaaacc tgcggcttga gttttttcag cggctgtgaa agataaagcg acaggttgta
     4021 atcgagtgtt attcgccttt ccgtcacacc gtaaacgagt gcggaaacaa atctcttatc
     4081 ttttatatca atctctgcct tatcgagcgc cgagtcaagc acaagatttg aatagctctc
     4141 atcacgctgt attttattaa gaatttcaaa cgctgtctgt cgggctgatt tcatataaat
     4201 ttccttttaa tttcatttct ttttgttctc ttagtacatc taatacnnnn nnnnnntcgt
     4261 caataacctt cagcgcacgt ctgcttgcgt taaattcaac gggcagagta acaaactgaa
     4321 aaacggcaac aagaccgtaa agaagaagtc ctgcgattat aacgtaatat ccgatcggcg
     4381 aggttgacag ataagcgaga aaataccccg caaaagcaat tataagtccg aatcttgagc
     4441 cgaaattcgt cacaggaaca agtgccgacc ttatcttatt cggcagataa tcctcggcat
     4501 gctgagcggc atgacctgcc tcgtgacaag cgattccaac ggcggcaacg gaggttgaat
     4561 tatacacctt ttcggatagt cttatcattt tttcgttcgg tgaataattg tccgtcagct
     4621 cgcctgcaca cggtacaata cgaacatccg taatgccgta atgctttaaa atcaattgag
     4681 ctgccgcagc tcctgtcagt cctcttgagt ttctgatttt ggaatatttt gaatatgttg
     4741 acttaactct tatctgcgcc aaaattgcaa aaataagcac gggagcggca aaaataagat
     4801 atcttatata ttctgaaaat gaataaatca tctaatcacc tcattcaaat ttttctccga
     4861 cctcaatttt atgtcctctc agaaaatcgg cacagctcat cgcttttttg ccgggagcct
     4921 gaatcattaa aattctgact ccgttgccgt cgccgcaggc aacagtcaat ccgccttgag
     4981 ttgaaacgat ttcgccgggt tcacccttag tttttccgat tttctctgtc ttatgaagct
     5041 tgtatatttc gccgtttaag actgatgttg caatcggcca aggggaaaga cctctgacct
     5101 gattgtgaat ctcatcggcg gattttgacc agtcaacggg gcaaagctcc tttgaaagca
     5161 tcggagcata atttgaaagc aagtcatcct gtttttttct ttcaagagtt ccgtcgataa
     5221 ttcttttaat tgtttgcgac atgacctctg cgccgagctt cgaaagctta tcatgcagct
     5281 cgcccgccgt catgttttcg ccgatttcgg tctccgcttt gataagcata tcgcccgtgt
     5341 caagccccga atccatctgc atggatgtaa cgccgctctt tttttcaccg ttgagaacgc
     5401 accattgaat cggtgccgct cctctgtatc tcggaagaag cgaagcgtgg atattgacac
     5461 agccgtattt cggcaaatcg agaatatcct gcggaatgat ttttccgtat gcgacaacaa
     5521 ttatcagctc gggatcaagc ccttttaaaa tcccgtatgc cgtaccgtcc ttcatcttag
     5581 tcggctgaaa aacaggaata ttatattctg ctgccttttc ctttacggga ggaaattgca
     5641 ttttatgacc tctgccctta ggtttatccg gctgagtaaa aacaccgact acgtcatatc
     5701 cgtcaaaaac gagtctttca aggcaaggga cggcaaattc cggcgttccc ataaaaacaa
     5761 ttcgcatctg caacagctcc tttgttatca gttgtcctca agctcaccta cgaggtggct
     5821 cgtgtaaaga attccgtcaa ggtggtcgat ttcgtgacaa aagcatctcg ctttgagatc
     5881 ggagccttta taaacgcacc acttgccctc acggttctgt gctttaacca taacgctctt
     5941 cgggcgctcg gtaacgccga atttacccgg aagagagagg catccctcct cctcatgctg
     6001 caattcgggc gatctgtccg taatctcggg attgataagc tcaaggtagc cgtctccgca
     6061 gtcaatcaca acaactcggc gaaggattcc gacctgaaca gccgcaagac ccgcaccttt
     6121 ttctttgtaa agagtatcct tcatgtcatc aagcagggta aaaagtctat cgtcgaattt
     6181 ctcgacgggt cgactctttt tgttgagaat aggatctccg aattttacaa tatttctgat
     6241 tgccatttca ataatcgaac ctttcaaaat caaaagacca taccgaaaat ttacgatatt
     6301 gccttaaatt atattttccg gatttatatc tatataaacg gatacatcat tgtacgccct
     6361 gtctgttcct gtatatttta acagctcgtt catcaaggct ctgaaacgct ttgtattctt
     6421 gcatttcaaa atcaatctgt atctgaattt attgcttatt tttgaaactc tcgccgtcat
     6481 ggggccgaga gcgattattt ttaaatcttt atattcaacg ctgagctttt ccttgagcct
     6541 gtcggtatat ttttgagaag cgaccctgac cttaggttcg ttctcaccgg taaaaccgat
     6601 aagacaaatg tcacagtacg gaggataaat gagcattttt cttgttttta attcatcttt
     6661 aaagaaagcg tcataatcct gacgtgcggc aatttgaata acgtcatttt cggggttgca
     6721 ggtctgaata accgcagttc ctttatattg tcctctgcct gccctgccga caacctgtgt
     6781 cagcaaatcg aacgtccttt cctgacttct gaaatcatcg ttattaagct gctgatcggc
     6841 atttatgaca ccgacgagcg ttacgttttc gaaatcaaga ccctttgcaa ccatctgcgt
     6901 gccgagaaga atatcatatt ttccgtcggc aaaatcctta agcttgtttt catgcgaata
     6961 tcggcccatt gtgctgtcgg catccattct gagaattctt gcttttggga ataaggccgc
     7021 aagctcatct tcaatcttct gcgttcccgt tccggaatac ctgacgtcat gcgagccgca
     7081 ctcggggcaa acatctgtaa acggctcgga atatccgcag taatgacaca taagcctacc
     7141 gttcgcatgg tgatatgtca ttgaaatact gcatgacgga caggtaagaa catgaccgca
     7201 cgccttacat tcggcaaagg tgttgtaccc tctgcgattg ataagcaaaa ttgactgttt
     7261 accgttttca aggttatatt gcagtctcga tctcagctcc tcactgaaat ttgaagactg
     7321 tgacagcgct tccgtcagca tatcgacaat gcgaacctcg ggaagaaccg agcttgaata
     7381 ccgttttgac agtctgttga ttgtgtattg ccctgcctgc gcctttgcaa atgtttcaat
     7441 gctcggcgtg gcgctggaga atacaagaag agccttatga taagcgcagc gaaaacgtgc
     7501 aacgtccttt gcgctgtatc tcggcgtctg ttcggatttg tatgtatgct cctgctcctc
     7561 gtcgattata atcagtccaa ggttttcaag aggagcaaat acggcacttc ttgtgcctac
     7621 aactatcctg acattttcat ttctgactct tttccattcg tcaagacgct gtcctgccga
     7681 gagagcactg tgaaaaacgg caacctctct gccgtaacgg ctcataaaaa gattaagagt
     7741 ctgcggcgta agagcaattt cgggcaccat aacgataaca tttttacctt tatcgagcat
     7801 ttcgtcaatc aatctcaggt aaaccattgt tttgccgctg cccgtaattc cgtaaagaag
     7861 agtagctccg cccatacccg aggaatattg cccnnnnnnn nnntattgcc cgagcatatt
     7921 ttcaaaggct ttttgctgtt cgtcattgag atggatctca tttctttgag gaacaacagt
     7981 cttgttttca tatgggtcac ggtaaatctc gttatcgaag atttcaacaa gacccttttt
     8041 ctcgagaacc tgtacgaccg aggctgtaaa cccagtgaaa taacaaagct ccttgacaga
     8101 aacagttccg atatcattta aaacgtcaag aacgccgatt tgtttttttg tcagcttctc
     8161 tgtcgtttca agaatctctc tgcctccctc ggtcagcctg acggtcttaa cggtcaagtc
     8221 gcccgttttt ctcacggaat cgaaatttct tatcagcagg ccttttttta ccattttttc
     8281 aagaacggta atttcaggtg taaatccaag cttttttagg agcttttcac cctctacata
     8341 gcccttttga g
//
LOCUS       DAWBSW010000083         8302 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1756_length_8302_cov_2.612465, whole genome shotgun sequence.
ACCESSION   DAWBSW010000083 DAWBSW010000000
VERSION     DAWBSW010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_8302_cov_2.612465"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2156 a         1838 c         1995 g         2313 t
ORIGIN      
        1 acacaacagg tgcagggctg cacaagaggg gataccgtcc gcaggcgaat gccgctccta
       61 ttaaggaaac gcttgccgcc gcactgtgcg accttgcgag acttcgccct tatcatacgc
      121 tttatgaccc aatgtgcgga agcggtacaa ttcttatcga gggcgcaatg atggttcata
      181 acattgctcc cggagtgaac agatatttct ccgcagaacg ctgggatgtc atcgacgaaa
      241 aggtttggac ggacgagcgc aaccgtgcga aagacctcgt aaattacgat acggatttca
      301 ttgcttacgg ttcggacatt gatgaggaag cgcttgaaat tgcaaaaata aatgccaaac
      361 gtgcaggcgt tgaaagaaaa ataaaatttg aaaaagctga tattcgcaac tttgaaccta
      421 agagcgaaaa gggtacggtt atctgcaatc caccatacgg cgagcgactg cttgatattg
      481 aaaaggcaca gaagctttac aagattatgg gcgagaaatt cataaaaaaa cacggctggt
      541 cgtattatgt tataagcccg tccgaggagt ttgaaaagga attcgggaga aaagcggata
      601 agcgtcggaa gctctataac ggcatgataa aatgtcagtt gttcatgtat tttaagtgat
      661 tttggaggta tttagtaatg aagcaccttt ttttaatcaa tccgaatgca ggtaaaggga
      721 aggcctttga aacggttatg ccaaagatta aagaagcaac tgccgaaaga aatatggatt
      781 ataaggtcta tgtcagcaag tcatcgcagg atacgcacga atattgcaaa aagataggtg
      841 agagcggcga gaaaacaaga atatatgctt gtggcggtga cggaacgata tatgatatcg
      901 tcaatgcgat ttatggctac aataacgttg agtttgccac tgttcctctc ggttcgggca
      961 atgattttat ccgtatcttc ggaacaaaag agcagtttat ggatataggc gctcagattg
     1021 acggaacggc aataaaaatc gacgctatta agtgcggcga cagaatcgcc gtcaatcagt
     1081 gttctatggg ctttgacgcc gaggtttgtt ccaaacaggc ggatttcaaa aaacttccat
     1141 ggcttacggg tgaatcggct tacacggctt cacttttcta ttgtcttaaa tcaaaaacat
     1201 ctaaccgctt tacaattaca attgacgacg gagagccgat aacggataat tttgtctttg
     1261 ccttctgcgg aaacagccgc ttctacggag gagggtatca ggcggcaccg tatgctgttc
     1321 ccgatgacgg actgctcgat ttttcaatcg tcaaagctat gaagctcccg aggcttatca
     1381 ctcaggtcgg gaactacaaa aagggcagac attacaaatg gccggagact acatatgtca
     1441 gaggaaaaaa gatgacggtt cacagtgata ctgcggcttc ggtcaatgtt gacggcgaat
     1501 gtgatcttgt cacggacagc acgtttgaaa tcatcccgtc tgcatacagc tttgttattc
     1561 ctacgacctc aagctatatt gaagacagaa aaagcggaac aatttccgat aaaataggtg
     1621 tggaaatcaa ataaaaggaa ataataaagt gactgcgatt attaaaacgc aactctttta
     1681 tttcggtgaa cactatggat atttttaatg cattaaccat gatcggcgga ttgtgtctgt
     1741 tcctgttcgg catgaacgtt atgggacagg cactggagcg ccgtgcaggt aacagcctgc
     1801 gaaacacgct tgaaaagctt acatcaaata aaatagccgg cttcctgacc ggtctcggcg
     1861 tcactgctgt cattcagagc tcgtccgcga ctacggtcat ggtggtcggt tttgtcaatt
     1921 cagggcttat gacactcagg caagccatca atgttatcat gggtgcgaat gtcggcacaa
     1981 ctgtgacagg ctgggtgctc tctctcggcg gaatcagcag cgataatgtt tggataagac
     2041 tccttaagcc gagttcattt acaccggtgt ttgctcttgt cggaattgtg atgtaccttt
     2101 tcatgaagga caagaagaaa aaggacagcg gtgcaattct gctcggattt tcggtgctga
     2161 tgttcggtat ggaaacaatg tcatctgccg tgtcgggact ttctgatgtt cccgagtttc
     2221 aacagctttt tgtgatgttt aaaaaccctc ttttgggact tctggcaggt actcttttga
     2281 cggcactgat tcagtcaagc tcagcttcgg tcggcatttt gcaggtgctg accgtcaccg
     2341 gacaggtttc aatcggtgcg gcaattccga ttgtaatggg tgacggtata ggaacatgtg
     2401 tcacggcgat gatatcggga gtcggaacaa agagagaggc aaagcgtgcc gcaacggttc
     2461 atttgctgat aaacgctatc ggctcaattg tttgggtaat ggtattctgg ctgattaaag
     2521 catttttgaa gcctgcattt ttagattcgc cggcaacgtt tgtcggaatc gctataataa
     2581 atacaggctt caaggtgttg aatacggcac tgtttctgcc gctttcggga gttttggaaa
     2641 agcttgtgtg caagcttgtt ccggattcga aggatgaaga agagagcacg gagcttgacg
     2701 agcgactgct ctttacaccg acgcttgcca ttcagcaatg cagcgtgaag gaaattgaaa
     2761 tggctcgtag agcggcgcgg gctctcaaaa acagcgtgac ggcaatcaat gcatactctc
     2821 atgaacttgc ggagaacata agagcggacg aacagcgctg tgaccgctat gaagatatgc
     2881 ttgcaaccta ccttgtcaaa atcggtgcgg aacggctttc tgatcgtgac agcgaggaag
     2941 caaacatgct gttgaagctg ctcggcgatt ttgaaagaat ctccgaccat gccgtgaatt
     3001 tgcttgaatc cgctgaggaa atgagagaca agaagcttgc attttcagcg gaagcagcaa
     3061 gggattttga aatcatttcc tcggcggtca atgaaatact tgacctttca atcgacgcgt
     3121 ttattaacaa cgaccgtgcc aaggcggagg acgttgaacc gctggagcag gtcatcgacg
     3181 agatgaagga aaatatgagg acaagccacg ttcttcgtat gagaaacggc gagtgcagca
     3241 tggaaatggg cttcgtctgg tctgatatac tgacgaattt cgagcgtgtc tccgaccatt
     3301 gctcaaatat agcaggctgc gttattgata cggcggagca caccctgaat ctccatgaga
     3361 cgcttggtaa tttcaaacat aacagtgaag aatttgaaga aagatataaa tattatttaa
     3421 aaaagtacag cacaatataa taattaaagg ttgctcggat ttaaccgtgc aaccttttta
     3481 aaatgatata tttattttac aatgcttacg gtgtaaccgc cgttgaacgt tacaactcgt
     3541 tccgccttag ataaatcggc tttcggagga taatccgtag aaacgatctg tgcaccgctg
     3601 tttagggcaa tctgcgaatc cgtttcctta tatgagccgt agttatccga tctcgttctt
     3661 attataagat tcttttcaag aacctcgtct gcctgaactt gaatatcctt gggcttgttg
     3721 atgagcagaa atgaagcata gtccttatca cggctgtcat agcgaagcat aggaaacatg
     3781 acctgggatt taattgaagt gtcttgcgca atgtatttgt ctgtcacggt cgtgtcatgc
     3841 aagagaagca taacatgacc tcttgtttcg ctgagtgtca tccaaccgtc gacctcacgc
     3901 atctccttga gtgatgaatg atttcccatc atgtcggcag gggttatgag cgtgtcgccg
     3961 aagatttctt ttgcctgttc gccaagctca ttggcataag cgcaggagaa ataatgcata
     4021 tttttgtccg gaatgaaaac ctttttcggt tcaatgatta ttgtaacggg gagatgatta
     4081 gggtttgcat ctgaccaaag cttgatttct ttcagcgcga gcgcaaaatc atagcaatgg
     4141 gttggactgt caagaacagg tgcatgggag caaacgaagc ttgtcttgcc gtcacttaca
     4201 accgtttcaa catcaagctc aacgcttctg actccgagat tgagctgatc ggtcagatag
     4261 tctgccgaga atttagcctt ttcggctttg acgagaccga acgttacggt cgaggcatcc
     4321 ctaaaaagct tttgccttgc cggaacacat tctttctgat aactgttgtg agtgccgaga
     4381 aacgaaactt cattgtattt tactccgtcg gcgcaggctt tatcgaggtc aaaatcacag
     4441 aatgaatttt cgtcaacctt agtataattc ccgctcttgt aatccttttc aagctgttcg
     4501 attcggtcga gctgttcctg aatgtcaatc tgcttctgtt cctgaacctg ttttatcatg
     4561 gcagggtatg taaaaacaaa gcagagaacg agagaaagaa gtgcggaaat atatttcgtc
     4621 atatatttca ttgtcaacag tcctttattc aataagatta tttgctgata aaagggcggt
     4681 tattgagccg cccttaaaca tttatttacc gtggtttgcg ggatttccgc agcacttttt
     4741 atactttttg ccgcttccgc aggggcaggg atcgttcgga ccgggagcag cttttttgcg
     4801 cactgttctg cccttttcgc ttccgtcgga agcgccgctc tcgcttgtga ccttggctgc
     4861 ctgctcacgc tttgtgtcct cctcacttct gatacggacg gtgagcattt gacggacggt
     4921 gttctcacga attgagttgc tcatctcatc aaacatatcg tagctttcca ttctgtatgc
     4981 aacaacgggg tcctgattac cgtaagcacg gagattgatt cctctcttga gctcttccat
     5041 tgcgtcaatg tgatccatcc agaaggtgtc aacgtttctg agaagaatca tgtgctcaag
     5101 gcttctcata atttcgggag tgaattcctt ttcacgggct tcatagcgct cgtggcctct
     5161 gttaatgagc atttctcttg cttcgtcacg gtcaaatgcg tcaatatcct caaagtcgtc
     5221 cggctctgta atccagccga ggtacttctc cttaaggcct gcgatgttcc attcatgctt
     5281 cggaagagcc gatgcacagt agaagtcgat tgattcggtc acggattcgt caagcatctt
     5341 gagaatcgaa gctttgagat cctcgccgtc gagaacacgg ttacgctgct cgtagataag
     5401 ctctctctga cgattcataa cgtcgtcata ctggagaacg ttcttacgga ttgcaaagtt
     5461 ctgagattcc ttcttcttct gagcggattc gattgaacgt gaaaccatct tggaatcgat
     5521 cggcatatca tcatctgcac cgagggtgtt cataacgccc tgaagtctct caccgccgaa
     5581 gagacgcata aggtcatcct caagcgagag atagaagcgg ctttcaccgg gatcgccctg
     5641 acgacccgaa cggcctctca actggttgtc gatacggcgt gaatcgtgac gctctgtgcc
     5701 gatgatgtaa agtccgcctg cttcacgaac cttttcggct tcctctttaa tagattcttt
     5761 aaatgagctt tcaagctctg catattcttt tcttgcggcg atgatttcct cgtcgtctgt
     5821 ctcggcaaag cctgttgcct caacgatcat ttcctcggaa tagcccttgc gacgcatagc
     5881 ggcttttgcg agaaactctg agttaccgcc gagcatgata tccgtaccac ggcctgccat
     5941 gttggtggca attgtaacgg cgccgaactt accggcctga gctacgatct ctgcttcttt
     6001 ttcatggaat ttagcgttga gtacttcatg cttaattcct cttgatttga gaagcttgga
     6061 aagaacctcg gatttttcaa ttgaaacagt accgacgagc acgggctgac ccttttcgtg
     6121 gcactccatg acctgattta tgagcgcatt atatttggca gcctcggtct tgtaaacaag
     6181 gtcgtggttg tcaatacgga tcatcggctt gttagtcgga atttcaacaa cgtcgagtga
     6241 gtagatctcc tggaattcct ggctctccgt catagctgta ccggtcatac cggaaagttt
     6301 cttgtaaaga cggaaatagt tctggaaggt gatggtcgca agagttttgc tctcgtgctc
     6361 aaccttaacg ccttccttcg cctcgatagc ctgatggaga ccctcgttat atcgtctgcc
     6421 gagcatgata cggcctgtga attcatcaac gataagaacc tgattgtcct taacaacgta
     6481 atcaatgtca cggcgcataa cgcccacagc cttgatggcc tgattgatat gatggagaag
     6541 agtcatgttc tcggcgtcca tgaggttgtc aacattgaaa taggcctcag ccttagcaat
     6601 accgctcttg gtaagagagg ctgttcttgc cttttcatca acaatgtagt cgccgtcggc
     6661 aacgtcctcg gcgttctgct tggagtcgat ctccttaaca actgcttttg taagagtgtg
     6721 aacgaagttg tttgcaaggg tgtaaagctc tgttgactgg tcgccgcggc cggaaataat
     6781 aagcggagta cgagcctcat caatgaggat tgagtcgacc tcatcgacga ttgcaaagtt
     6841 atgtcctctc tgaaccttgt tctccttgta cggaaccatg ttgtcacgaa gatagtcaaa
     6901 tcccatctca ttgttcgttc cgtaggttat atcagcggcg taagctgcct tacgctcatc
     6961 gttgtcaagt ccgtgaacga taaggcccac cttgagaccc atgaacttat aaagcttgcc
     7021 catccactcg gagtcacggc gtgcaaggta atcattgacc gtaacaatgt gaactccgtc
     7081 acccgaaaga gcgttgaggt aagcggggag ggtagcaaca agagttttac cttcacctgt
     7141 tttcatttct gcgattcgtc cctgatgaag aacaataccg ccgatgatct gaaccgggaa
     7201 atgcttcatg ccaagaacac gccacgaagc ctcacggcat gcggcaaaag cctcgggaag
     7261 aatatcgtcg agcgtttcgc cgtttgcaag tctctccttg aattcgggag tctttgcctg
     7321 aagctctttg tccgtgagct ttgcatactc ctcttcaagg gcaagaacct tattttttat
     7381 cgggactacc cgcttgactt ccttagcgga ataatctccg aagatttttt taagaagacc
     7441 catattttca ttcctttcag aaattgaatt taaattagtt tcttcacgga agaatgaggt
     7501 acaatgtgca atctcaaatc aaggcttccc tttgagagga aggctcgtca aaaatgtctg
     7561 atagttgtca acaaagtaca atatttcaaa gtttttcgtg aagagtctta tattaaccta
     7621 ttaaatatat cacaaaaaag caaattatac aagactttcg tcaaagttga ttgtaatttc
     7681 tttgtgcgga agagtcaact gtttgtattc cactcctgcg gcgtcgaaca tacgcttgga
     7741 ggcaaggttg ccgctcgttc cgttatactt gtcggaaagg tagaaaacct ttttgattcc
     7801 gcactggatt attgccttgg cgcactcgtt gcaggggaaa agagcaacat aaacgctgca
     7861 tccgttcagc ggagcgccgg cgttgttgag tattgcgtta agctcggcgt ggcaaacata
     7921 cggatatttt gtgtcatagt cgttatctgc ggttctgtcc cacgggaagg agtcgtcgct
     7981 gcatccgagc ggaaagccgt tatagccgac cgacataatt tttttgtttt catttactat
     8041 acaggcacct acctgtgtgt ttgggtcctt gcttctcttc gcggcgagca aagcaacgcc
     8101 catgaagtat tcatcccacg aaatataatc ctctcttttt gacattgttt tccctctcaa
     8161 tctgcgttga atttaacaat acggtttatt gctgcgccat cctcggtgaa gtttgacaca
     8221 agaaccgttt ggttgctgat aaagcaataa ttgcatattc cgctgttgaa aatgtcgctg
     8281 tcggaaataa tgctcgcttt gc
//
LOCUS       DAWBSW010000084         8235 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1773_length_8235_cov_2.856968, whole genome shotgun sequence.
ACCESSION   DAWBSW010000084 DAWBSW010000000
VERSION     DAWBSW010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1773_length_8235_cov_2.856968"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3913..3922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5778..5787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7767..7776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2487 a         1664 c         1981 g         2073 t
ORIGIN      
        1 attcgggaca gaactacacc gtagccggca aatttgaagc aggctggaac agagaaggca
       61 cagccactct gacggcgcac tgaaatacgt aacggcgaat cagactgaat gatttaccga
      121 aataccgccc cgaaccgaag gaattcggct cggggcggtt atctttttac aaagtaaagc
      181 cttgcaaatc atatgaaatg caaggcttta atgtatttta atatgtttat tttaaatatt
      241 tttcaataaa gaatctggga cgccctttgg tttcaagata aatctttccg atatactcac
      301 cgacaacgcc tatggcaata agctgcaacg agccaaaaaa ccaaatggaa aggactgtag
      361 atgtccagcc cggaactgtc cttttgataa aatagcttat caaggcatat aatgcgaaaa
      421 ttatatttaa aataagtgtt gccccgccga gagctgttat caaacggatt ggttttattg
      481 aaaaagatgt tatgccgtca aaagcaaagg tcagcatttt ttttagcgga tacttagatt
      541 ctccggcaag cctcggcgca cggtcatagg tgcccgtgga ggacttaaag ccgacaagcg
      601 gaaccatacc tctgagaaaa agattgactt ccttaaattc gagaagcgcc tttactgctc
      661 ttgaactcat cagtcggaaa tcggcgtggt tatataccgt ttcaacgccc atcattgaca
      721 taaatttgta aaagccctga gctgttgttc gcttaaaaaa tgtgtccttt tcccttgaag
      781 cacgaacgcc gtaaacaatt tcaactccgt tattgaaatc atcaaccatt ttatcaatag
      841 cattgacgtc gtcctgagcg tcggcatcaa ttgtgactat catatcacaa tcatcgacta
      901 ttgttgacat gcctccgaga agagcgtttt ggtgtcctct gtttcttgaa agcttaatgc
      961 cgacaaattt cgggtcaagc tcatgcaagc tttcgataat ttcccaggtt ttgtcctttg
     1021 aaccgtcgtc aacaaaagcg atgcggctgt tttcacttat tttaccttca tcgataagtg
     1081 aattgacctt atcgagaagg atttttgatg taagaggcaa tacttcttct tcattatagc
     1141 agggcgcaag aatatataaa gtcttaggtt ccacagtcag tcctccttta attcaaaaag
     1201 atagtacgtt tggaccgttt ttttattttt gaatgccgat tcggcaataa tgtactcttg
     1261 agagaaaaca agcgtataat cggtctttag tgcgatattt ttattgtaaa tatcggaaag
     1321 ctccatgccg gatttagcgg caatagcaac ataatcggcc aacccgtcgg aaatgtatct
     1381 gttttgttcg tcaatattct cgggatattt atcgtataaa agattctgct gctcaaagtg
     1441 tttaaattgc ggcacctgtt tcgcggcaag ataaatgccc gaatccaaag agccgtagtt
     1501 gagaatcctg ccgttggggt tgcgcgagac gatgtattcg gctgctttgg attgtactgt
     1561 ttcttcgccc ttcctcgggg taaaccttga tgatagattt aaataatttg aagtaactat
     1621 tgccgtcgga ataattgcga tgcaaagaat agccgcaatt attcccgatt tgctttttgt
     1681 aatattattg agcaaatatg ccaaagcgat aaaggtaaat actgtgaata cggacatcgg
     1741 aataaaatag taattatagt atcttattcc gaaataaatt atatacagcc cgatacagca
     1801 aacaaacgga acggaaagcc gagagaaaag ctttttctcg gtaaaaattt ttgttgcggc
     1861 aaaaatgatt attccgagga atccgaggat aaacaaagca ggtgaaatca caagattttc
     1921 gcacaatgaa tgtaacggtt caaaaattcg tgaaataatt ccgagtccct ctgccctggg
     1981 atatgccgtg gcattgagaa caaaataggt gttgatgaaa tcatacagag cgcctttgac
     2041 agcaaaatat actatccacg gtgcaacggc aagtgccatt ccgcagagaa aaatcatagc
     2101 attaacgaag gcttttttga aattcttttt tgcaatccaa atgtaaagca aaatgtatgc
     2161 cgcccatgcg aaccaaaaac cgataacggt aaattttatc attgcaacac agccggcaaa
     2221 aaaaccgacg agaaggaaaa cggacttgct aagctcatct tgttcggtgt ttctgaaata
     2281 tcggcagaaa aaataaatgc tgatcatcaa caacggcagg caaaattcct cggcactgtc
     2341 gccgtaataa tagcagccgg aattgacggt aacaaagcag gtgagcacag cggaaatcac
     2401 cgaccatgcc gttccgacat aaagcgaagc aattttatat gcggcaaatg cagtcgccga
     2461 aagaaataga gattgaataa tataaactcc caaaaacgag gaatcggaaa tcagacttgc
     2521 gacggcatga agaagataaa gcaacggccc cttttgctca aaaatgtctt tgtaaattgt
     2581 cagaccgctt gacattgcac gtcccattgt cagaaatgca tttgcgtcat tccacggatt
     2641 cgtggcataa cggggtgacg atgcggtgca gatgtaaaga ataccgaaag aaaaaatgag
     2701 aaaaagcaaa taaacagcaa aagtcataag cttgtttttg cttgataaca cactttgttt
     2761 tttcaatatt ttcaccaccg ttcatacata catgaattat atcattacac aaagcttaaa
     2821 gtcaatattt attaaatatt atattataga atgttataaa agacttgaag caaaagattt
     2881 tatattaaaa agcttgaaat aatacagttt ttattatata attaaacggt taataagtta
     2941 aaaagagagg tatgcccttt gagcgaaggc acaatgaaaa agattattaa ggtttcgcca
     3001 tccgagatgg agaagcagcg tgaatataca gagcttgtca ggacggtttt gacctcaaga
     3061 tttgagcctt ctcagccgct tgcctttgtt catacctatg gttgccaggg caatgtttcc
     3121 gacggagaac gcattaaggg aattcttgaa acgctcggct acggcttcac cgatgatgtg
     3181 gaaaaagcag acttgattct ctacaacacc tgtgcggttc gtgagcatgc cgaggacaga
     3241 atttacggca acgtcggcgc aatcaagagg ctgaaaaaga ccaatccgaa cttgataatc
     3301 gcactctgcg gctgcatgat gcagcagaaa agagttgttg ataaaatacg aaaaagctat
     3361 ccttttgtca atcttgtttt cggaactcat gttattcaca gaattcccga gctcatttac
     3421 cgtgtgcttt gtacgggaaa aaaggtctat gagtgccccg atgaggcagg cgttattgcc
     3481 gaggatctgc cgataaggcg tgacggtaat atcaagggct ggcttccgat catgtacggt
     3541 tgtaacaatt tttgcagcta ttgcattgtt ccgtatgtaa ggggacgtga acgcagccgc
     3601 gagcccgagg atattatcaa ggaagcaaag gagcttatcg catcgggctg caaggatata
     3661 acactgctcg gtcagaatgt taattcatac ggaaaaaatc ttgacagaga tatcaatttt
     3721 tcaaagcttc ttcgtcagat caacgatttg ccgggtgatt ttataatcag atttatgacc
     3781 tcgcacccta aagactgcac aaaggagctt cttgatacaa tggcttcgtg cgacaaggtt
     3841 gcaaagcatt tgcatcttcc gttccaaagc ggaaatgaca gagtcctcaa ggcgatgaac
     3901 cgaggctata cgnnnnnnnn nnacccgatt tcaagagagg aaaaaggcaa atggttcaag
     3961 gagctgacgg acgcacagga gaaaatcgcc tccgagcgaa cggcagctat gaaggataag
     4021 acctatacgg tgctttgcga gggttatgcc aaggacggta ttctcaacgg cagaaccgag
     4081 ggcaacgtta tgatagagtt ccccgaggtc gagggtaagg agcttatcgg aaaattctgc
     4141 aaggttaggg ttacggagcc cttgacatgg atagtcagag gagtattgga tgaacgctga
     4201 aaggcgtttt tcagataaat aaatttatag gagttttaca atggacatta ttgaaatcac
     4261 aagacagctc ggcgcagcga ttcagcagga cgagcgttat cttgcattcc atgaggcaag
     4321 aaagacaaac gaggctgaca cagatctcaa cgatcttatc aataagattc agcttatcca
     4381 catgtcatat cagcacgagg cggccaagga tgacgcaaac gaggagaagc tcgctgctta
     4441 cgacaaggag tttacagagg tttatcaggc agttatggca aacgagaata tgcagaagta
     4501 tgaggccgca cgtcaggctg ttgacgagat gatgaatcat attacaggta ttcttgccct
     4561 ctgcgttcag ggcgaggacc ccaaaacctg tgagccgcag gaggagcatc attgctcggg
     4621 cgaatgcggt tcatgcggcg gctgccacta agatggattt tagattttag atattagatg
     4681 ttagataata tttatgatat tctcagtcta atatctaaag tctattgctt gaaaaatgac
     4741 gaagtttaat ttatgaggtg aaaaacagat ggcagaaatg acgccgatga tgaagcaata
     4801 tcttgaaatc aaaaaggaat atcaggatac gattcttatg ttcagactcg gtgactttta
     4861 cgagatgttc tttgaagatg ccaaggttgc gtcgagggag cttgagcttg tgctgacggg
     4921 cagagactgc ggtcaggcag aacgagcgcc catgtgcggc gtgccttttc acagcgttga
     4981 ttcatacatc gcaaggctcg tttcaagggg ctataaggtt gctatctgcg aacagaccga
     5041 agaccctgca acggcaaagg gtatagttaa acgtgacgtt atccgtgtgc tgactcccgg
     5101 tacggttatc gagagcagca tgcttgacga atcgaagaac aatttccttt gttcaatctg
     5161 gctcaacggc aatgctgcgg gcgtttgctt tgcagatgtt tcaacgggtg agctgagcct
     5221 tacccagctt gattcaaaat cggtcggtaa tgagatcgtc aatgagctcg gaagatatat
     5281 gccgacggaa attcgctata attccgccgt tgttaagaac gataaaatca gggagttcat
     5341 aaaaaaccgt gttgaatgta ccgccgacag tattgacgag gagaaaatca atgttgatta
     5401 ctgccgccgt gttataacaa atcattttaa aaagagctta accgagcttg ccattgagga
     5461 aagtaatgag gctgtttgcg ctctcggcgc aacgaacgat tatttgaccg aggtgcagaa
     5521 atccggcctt gataatattc gtgagattga cttttattcg gaaaatcagt ttatgaaaat
     5581 tgatttttct tcccgaagaa atctcgaatt gaccgaaacg atgcgaaaca gggaacgcag
     5641 gggaactctc ctgtgggtgc ttgacaagac aaaaaccgca atgggcaagc gccttgtcag
     5701 aaactggatc gagcagccgt tggtcaatct tgcgaagatt accaagagac acaatgccgt
     5761 cggtgagctg tttgacgnnn nnnnnnnaga tagcggcgat ttcaaacgtc agcgacatgg
     5821 aaagactgat ggcgagaatt gtttacgaaa ccgcaaatgc gaaggagctt cgttcgcttc
     5881 ttgcggcgat acagcttttg ccggatatta agagcggaat tgccgggtgc aaatctgcgc
     5941 ttctcaacga gacgtgcaga agcattgacc ttctgacgga tgttgccgag cttattgaca
     6001 aggcaattca ggaggagccg ccgtttacag tccgtgaggg cgacatgata aaggacggct
     6061 ttaacgccga gcttgacgag ctgagggata tcgtacataa cggcaagggc tatattgccg
     6121 ccattcagca gaccgagcag gaaaaaacgg gaatcaagaa gctgaaaatc ggctacaatc
     6181 gggttttcgg ttactatatc gaagtgccga atgcatttaa agagcttgtt cccgaggaat
     6241 atataagaaa acagacgctt gcaaactgcg agcgatttat aacgagcgag ctgaaagacc
     6301 ttgaagccaa ggttctcggc gcacaggaaa gaatcgtaaa gctcgaatat gaaattttcg
     6361 ttcaggtcag aggtaaggtt gccgacgagc tttacagaat ccagaaaacc gcccatgcgg
     6421 tcgcaacaat tgatgcactt tgttcctttg caaaggttgc tcagcagaac gactattcgt
     6481 gcccgataat gacggacggc gacgaaatca ttattaagga cggcagacat ccggttgttg
     6541 aaaaaatgat gagtgatacg ccgtttgtgc cgaatgatac ggcgcttgac tgcaaggaca
     6601 accgttgtgc gataatcaca ggcccgaaca tggcgggtaa atcgacttat atgcgtcagg
     6661 ttgcgcttat cgtgctcatg gcgcagtgcg gcagctttgt tccggcttca agcgcacaaa
     6721 tcggcttggt tgacagcata ttcacccgag tcggtgcgag tgacgatctt gcgtcaggtc
     6781 agtcaacgtt catggttgag atgagcgagg ttgcaaacat tctgaaaaat gcaacgaaga
     6841 aaagcttgtt gatttttgac gaaatcggtc gaggcacttc aacctttgac ggcatgagca
     6901 ttgcacgtgc cgtgcttgaa tacaccgccg acccgaaaaa gctcggcgcg aagacgcttt
     6961 ttgcaacgca ttatcatgaa ttgaccgagc ttgaaaacga aatcggcgga acgaagaatt
     7021 ataacatctc cgtcaaaaag aggggcgacg acataacttt tctcagaaga attgttcgcg
     7081 gcggagcaga cggcagctac ggtattgagg ttgccaagct cgcaggagtt ccgaatgctg
     7141 ttgtcaaccg tgccaaggat attctcaagg agcttgagga aaagggtgcg gttacagtaa
     7201 taagaacggt tgaaaagagc gatgacgata tgcaaatttc attcgcttcg aacgagggca
     7261 atgagattgt tgaggaactc aagacgattg atataaacac gcttacgccg attgaggctt
     7321 tgcaaaagct ctatgagctg aaaaacagag cggacaggct gtaaaaaaat aaggaaagaa
     7381 aaagaggtga tcttatgccg caaataaata ttttacccaa gcatattgcc gagctcatcg
     7441 ccgcaggcga ggttgtcgag cgtccggcat ccattgttaa ggagataatg gaaaatgcga
     7501 ttgatgcagg agcggacaag atcacacttg aaataaagcg cggcggaatt acatatatcc
     7561 gcataaccga taacggctgc ggaattgacc gtgacaacat tcgcaaggcc ttcatcagtc
     7621 atgcgacaag caaaatttcc accgctgacg atttgaatgc gatttgtacc cttggtttcc
     7681 gcggtgaagc gctggcttca attgccgccg tttcaagagt agaggttatg acgaggggcg
     7741 aaaacgagga cgtcggaaca cggtatnnnn nnnnnnggca acgcagttgc ggcggtcgtt
     7801 gacagaattg cactttctca ccccgaggtc agcgttcgat tcatcagaga ctcaaaggac
     7861 gtgcttttca cttcgggtga cggaaagctc gacagcgcaa tatataaggt gctcggcaag
     7921 gattttgcat caacgcttat tccatgcgac tacgagctgg agggtgtgag agtcagcggc
     7981 tatatttcaa agccgttcaa tgcccgtccg aaccgaatga tgcagtattt ctttttgaac
     8041 gacagattta taaaaacacg tacaggtatg gttgcccttg aggaggcata taaaaactca
     8101 atcatggtcg gtaaatttcc tgcctgtgtg ctcaaaatac agattgctcc cggtgcagtg
     8161 gatgtcaatg ttcatcctgc gaaaaccgag gttcgtttcg ccaacgaaaa gatgattttt
     8221 aatgcggttt attac
//
LOCUS       DAWBSW010000085         8192 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1789_length_8192_cov_2.778542, whole genome shotgun sequence.
ACCESSION   DAWBSW010000085 DAWBSW010000000
VERSION     DAWBSW010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_8192_cov_2.778542"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1899..1908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2966..2975
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5893..5902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2375 a         1640 c         1955 g         2192 t
ORIGIN      
        1 cggtaaccgc cgaaaagctc agagtcatca aaacggcggg agtcggcaga ataagcataa
       61 atccgcagac ctttaacgat gatgttctca aagccattgg cagaaggcat accgttgacg
      121 atgttgttcg tgttttcaat gaagcgagag aacttggctt tgacaatata aacatggatc
      181 ttatcgcagg tctgacggac gatacatatg agagcttttg caattcgatt gaccgtgcga
      241 taagaatgaa tcccgagaat attaccctcc acacgcttgc tttgaagcgc tcatcaacta
      301 ttgttactca cggcgttgac gttcaaagcg gggaaattgc aaataaaatg ctggaatatg
      361 ctcaaaatcg gttctattcc gaggggtata agccgtatta tatgtacaga cagtcgcgtt
      421 cactcggcaa ccttgaaaac gtcggctggt gcagggacgg atttgaatgt ttatacaata
      481 tttttatgat ggaggagtgc catacagtgc tcgccgtcgg tgcgggagcg gtaacaaagc
      541 ttaaagatcc gaacagcaga aatattgaaa gaatttttaa ttataaatac ccatatgaat
      601 ataattcacg ctacgatgct attgccgagc gtaaggatca aataaaggag ttttacggta
      661 agctgagatg agaggtagca aaatggcata cagaacttta aatgatataa ataaaagcgt
      721 tgcttcggat gttgagtcgt ttatcgaatc ggcagaggag ggctttgaca gctctgtttc
      781 ttcactggtc ggtaatttca tttccgattg cgactgcgat atcgttctgc ttgcaggtcc
      841 cagctcatcg ggaaaaacca caacggcagg aaaaattgta gaaaaaatca gggacagcgg
      901 cagaaatgcc tatacacttt ctcttgacga ctattatcac aacgcagccg atattccatt
      961 gaccgcaaag ggtgtcaagg atttcgaaaa tatcaatgct cttgatgtgg atttgattca
     1021 cagaaatttt tcggatttaa ttgaaaaaag agaagcgctt gtgccggaat ttgattttat
     1081 gagcggtaca aggaaaggca aaatgagacg cattgagctt aaaaaggacg atgttatcat
     1141 tgttgaggga ttgcatgctc tcaaccccgt tattaccaat ggacttgacg aaagccatat
     1201 ttataaggtt tatatcagtg tttcatcgag ggtgacggat gacgagggca gggtggtttt
     1261 cagcaagaga aatcttcgcc ttatccgtcg gatgataagg gattatcact acagaaacac
     1321 atcggttgaa aagacttttt cgcagtggcc ggaggttctc aagggagagg ataagtatat
     1381 ttttccttat gagaagaatg cacgctacag aatagattcg ttccacccgt acgagtcgtg
     1441 tattttcaga aacgaagcgg ttgagctgct tgacacggtt ggaaagagca gccgttttta
     1501 tccgcttgcg gaacagctta aagaagattt ttcaaggctc aagccgatag atatatctaa
     1561 aatgccgaat gattcgcttt tgagggagtt tgtgtgagtt tacagcaaag tcggcagaaa
     1621 ctaattttgc tgccaatgta tgggcgttaa gaagatttcg gtgccgaaag aatatatatc
     1681 aaacttcttt ggtgcgatga agggcagcac atcctacgat aagagcgata aaaggaacaa
     1741 ttcgtagtgg tcgatgcctc ctatattgac caagggtcag aagaaatcta ccaaggcttt
     1801 ccttgagagg aaggctgata agttatcact ctgaaagcgt ttttatctga gttatggcat
     1861 gaacggttga aaaatttaga acatgcctct cgcatcttnn nnnnnnnntt cccacacgaa
     1921 atatttgata aacatattga taaataagga aatatatgat aaaataattt ctgccgtttt
     1981 tacaatggca ttaaaaagtt aaaattttct ttctaaatat aacagtattg aacaggagga
     2041 aatacgatgg ctgaagaaaa gtcgggcgca agaagtcagt cgcttctcag cggagctttt
     2101 atactcgttg ttgccactgt tcttgtcaag gttatcggtg cactgtttaa aatcccgctt
     2161 tccatgctaa tcggagaggt cggacgagga tatttcagca cggcttatga aatatacact
     2221 cctctttatt ctatctcaat ggcgggctta ccggttgctg tttcacggct cgtcgcaaag
     2281 gagcgctcgc ttgggcattt ccgtgatgtc agaatgataa ggcgtgtttc ggctcgtctg
     2341 tttctgctga tgggtgttct cggaacggtt ctgttaatgc tcgttgccta tccttataca
     2401 aagttcattg tcaagtatgt tgaaaatgtt tactgtgtac ttgcaattgc gccttcgatt
     2461 ttcttttgtt gctgtatgtc aacttatcga ggctattacg agggtatgca gaatatgctc
     2521 ccgacagcga tttccgaggt catagaggtt gtcggcaagc tcatagtcgg tctcggtgca
     2581 agctatgcac ttctcaaaag tcagcttgcc aggtttgaag ccggtcagcc ggttttcgga
     2641 gcaattcgtg aaacaaaaga ggatgccctt gctgcaattt atccttatgc ggcaacaact
     2701 gcggttctcg gggttacctc gggcacggtt ttaggtcttg tttatcttat tataaggcat
     2761 aggttgaagg gtgacggtat aaccaaagct cagcttcttt cttctcccaa acctgccgac
     2821 ggtcaaactc ttgctaaaat aatagtcatg acggctctgc ccattgcggc aagctcactt
     2881 gttacgaata ttacaaatct gattgacgcc gcaaccattc aggctcggct tcaacatgcc
     2941 gtcgaaaaag ctcccgacgt tatctnnnnn nnnnncctct acggggcgga attcgatgag
     3001 atgaaggttg ccgattcgca gattgttaaa tatatttacg gtatttataa cgaggttctt
     3061 gattttaaaa atcttgttcc gacgattacc atgacgctcg gcatcagtgc cattcccgtc
     3121 ctttcggcgg cgtgggccaa tcaaaagcgt cacggcatta agctggccgt tgagtcggtt
     3181 atcagagtta cgatgcttat ttccctgccg accggattcg gcctggcagc ggtctcgtat
     3241 tcgcttctgg atatgttcta tcctgccgcg acggcgagca taggctcaac gatactggct
     3301 gtctatggct gtacgatttt catttttgcc gtttcttcgc cgattacaag tatgcttcag
     3361 gctatcggca gaaccgatat tccgttgaag tcgcttgtcg ttggagcggt tatcaaaatc
     3421 gttctcaatt gtattctcat cggcaatccg catataaaca tcaacggtgc gccaatcgct
     3481 tcaattgtat gttaccttgt tgttgttatc atcaatttgt acagcataat caagattaca
     3541 gaggttaaga taaatttcgg ttccgttttt atcaagccct gtatctgctc ggctatcagc
     3601 gcatttgtgg ctttcctgac aaatcttgtt ttcggaaagt tccttggcag tctcggcaac
     3661 accgacagta tgctgaacgg caatacggtt gctttaataa ttgcggttat tgcggcggtc
     3721 attgcatatg tcattacaat acttcttttc aagggaattt caaaggacga tcttcttatg
     3781 ataccaaaag gagaaaaaat tgccgaaact cttgaaaaac gtggattttt agggtaaaat
     3841 aatatggata tgaaaaacag ttttaaattc aaagataaat acaatgttta tgatcttgtg
     3901 gagatattga gagttctccg ctctgagggc ggttgccctt gggatatcaa gcaaacgcat
     3961 gaaagcataa agaaaaatct tattgaggag acctacgagg caattgaggc tatcaataag
     4021 aaagatcccg atatgcttcg cgaagagctg ggtgatgtcc tgatgcaggt cgttttccat
     4081 tctcagatcg aggcggaaaa aggcgtgttc gatatcgacg atgttgccga tgagaactgt
     4141 aaaaagctta ttgagcgtca tccccacgtc ttcggcgagg tgaatgtcaa cggcgttgac
     4201 gatgtgctga caaattggga tgccatcaag cgcaagacca agggtcagaa atcaacctcg
     4261 gaggctattg actcgatccc gagagagctg ccggcgctca tgagagcgac aaaggttcag
     4321 cagaaggcgg caaaggcagg ctttgactgg gatgatgtcg gcggtgctct tgataagctc
     4381 agtgaggaaa ttgcggagct taaagcggcg atcgcaagca acaataaggc gaacataaat
     4441 gaggagctgg gcgatgtcct gttctcggct gtcaatgttt cgcgctttgt cgatacggac
     4501 gccgaggagt cgcttacgga cgcaacagac aaattcgtca gccgctttaa aactgttgaa
     4561 aagctcgcca aggagcgaga tattgacatg aaggagtccg atattgagac tcttgacagg
     4621 ctttgggatg aagcaaaaca ctgatttaag taaatgatag ctttgaaacg ccgcggtgca
     4681 tcggcagtat caaagatttc ataataaaaa actggaggaa ttacaatgaa taagacagaa
     4741 tttatcaatg cagtagccgc taagggcgag atggacaaga aggctgccga gaaagcagtt
     4801 aacgctgtta tcgctgcaac aacagacgca ctcaagaacg gtgagaagat ccagcttgtc
     4861 ggcttcggca cattcgagat cagagagcgc tcagagaagc aggctcgcaa cccgagaaca
     4921 ggcgagacaa tgactgttcc ggcaagcaag gttccggctt tcaaggcagg caaggctctc
     4981 aaggacgctg ttaaataatt ctcttttact tcgattgtat cgggcaggac agatgctttt
     5041 gtcctgcctt atccaattaa aaaaagaaag gtatttaata tgagactgga taagtatctc
     5101 aaggtttccc gtgttatcaa gcgcagaacc atcgcaaacg aggtctgcga cgcaaagcat
     5161 gtccttgtca acggaaaaat cgcaagagct tcctatgacg ttaaggaaaa tgatatcatc
     5221 gagataacca tgggctcaaa tgttacaaaa atacaggtga ccaaggtttc ggagcatgcc
     5281 acaaaggacg atgcggcttt gatgtacaag gtcatagatt aaatcagaat attatagtat
     5341 ttttaatgcg ccgacctcat atgtttatat gaggtgtttt ttatgtcaga agcattgatg
     5401 cacaatattg ttatagaaaa ccgacagcgt ttgagcgcaa cgggaataca gaatgttgac
     5461 agctatgagg atgactgcat agttgctcaa agtgagtgcg gcgaaataat tatcaaggga
     5521 catgacctta aaattacccg tctttcggtt gagacgggcg atatgacggt cgagggcagt
     5581 attgattccg ctagctataa tgcgccgaag gtcagcggaa gctttttcag cagggtgttc
     5641 aaatgattga atacctccct aaaacctccg aacagctctt gacaataatt tattccgtcg
     5701 gaatcggctt tgcactcgga ttattctatg aatttttgag attatttttt tatctgctgt
     5761 caggaagcga taaaaaattc actcttttaa gagatataat atttttactt ttcgtcctca
     5821 ccgttacatt cttctttttc attgtccgct gtaacggcag ggtgactttc tattccgtaa
     5881 caggtgaggc ggnnnnnnnn nncgttttta ccgaaaaaat caaaaagaaa caaaaaaata
     5941 tcgcaaaagg taaaaaaatt tcggaaaatg acttgcataa tcggcacgat atagtgtata
     6001 atcaatccgt aacggtgcgc ccgttaattg atgaaacaga gaatcgaggt gactgaaatg
     6061 gcagaaaaga aaaagcgcaa gcgcagcttt attgtatcgt tttgcataat tgctttgtgc
     6121 gcatatttcg ccatttcatt gataaccata catagaaata ttaaagaaac caaagctgaa
     6181 attgccaatg tcaaggcgtc ttataacgag caggttgccg aaaacaatcg tatcaaaaag
     6241 cgcattggcg gcggtgaaat agatgaatac gttgagaagg tcgcaagaga tgagcttggc
     6301 tatgttaagc ccggtgagca tgtttattat gacgtctcgg tgaatgatta acggttcgga
     6361 aaaaagagga gtacgcattt tatgcagttg gaagttggtt caatagttga gggaaaagta
     6421 accggtttta cgtctttcgg cgcttttgtc gatcttgacg gcggcaaaaa gggaatggta
     6481 catatttccg aggtcgcaac ggaattcgta aaggatatca aggatcatct caaggtcggt
     6541 cagcaggtaa aggtaaagat tctttccatt gcggacgacg gtaagataag cctttccatc
     6601 cgaaaagcag ttgaacagcc gaaaacccca aggccgaagt ccaatcagcc gaggagacct
     6661 gcggcgaatg tttggcaggg caacaagagc acggacaatc agcctcagtc atttgaaagc
     6721 atgatggcaa agtttaagca ggtaagtgac gagaaaatgt cggatctgaa gcgtgactcc
     6781 aagcatggcg gcggatataa ccgccgaggc acaaataagc tgtaatgtaa tttcaggagc
     6841 aggtttctgc tcctgttttt tatattggca accgctaatt agcttgccgg aaagaataag
     6901 ctaatattga aaatgattgt agcacaactc tcagcggctg atctataatc ggaatatcga
     6961 aacattcagg tgatatattt gagagaaatt gatgtaaata aaataagcga ggcggtcgca
     7021 gagctttgcg tcaaggcgaa caagcttctg cccgatgatc ttgcagaaac aatagtcaat
     7081 tgccgcaaat gtgaaaagaa cagacttgcc gaaagcgttc tcggcgatat cgaagccaat
     7141 cttgatgcgg caaaggagct tgatattcct atctgtcagg acaccggaat ggcggttgtt
     7201 tttcttgaaa tcggtcagga cgttcacttc gttggcggca gccttaacga agccgtaaac
     7261 aagggcgttc atgacggtta tgtaaacggc tatcttcgct gttctgttgt cggcgatccg
     7321 ctcagacgtg tcaacaccaa ggacaacacg cctgctgtta ttcacacaca tatcgttgac
     7381 ggcgataaag tgaaaatcac cgttgcgccc aagggcttcg gcagcgaaaa tatgagctgt
     7441 cttaaaatga tgacgccggc aaggggcagg gaggacatta ttgaatatgt tctttcgtgt
     7501 gtcagaaccg caggcggaaa tccgtgtccg ccaatcgttc tcggaatcgg aatcggcggc
     7561 gattttgagc agtgtgcata ccttgccaaa aaagctcttt gccgaagcgt tttgaaaaaa
     7621 aatcccgatg aattctatgc tcaaatggaa gcggaaattc ttgaacgcat aaatgcagag
     7681 acaaatgtcg gaccgatggg cttcggcggc gatacaacgg cactttccgt cgccatcgaa
     7741 acagcaccga ctcacattgc cggattgcct gtagcagtta atttcggatg ccatgtaaca
     7801 cgtcactcta cagcagaaat ttgaagtgat ttgacggatc tttttccgtt atacagccta
     7861 aagccctctc ggcatataag aatatagcct tcgagaactt taaactgtct aacaaaaaaa
     7921 tctctcgtca aaaggtttgc accttttccg ttatacagcc taaagccctc tcggcatata
     7981 aggatatagc cttcgaaaac cttaaactgt ctaacgaaaa aatctctcgt caaatggttt
     8041 gcaccttttc cgttatacag cctaaagccc tctcggcata taaggatata gccttcgaaa
     8101 accttaaact gtctaacgaa aaaatctctc gtcaaatggt ttgcaccttt tccgttatac
     8161 agcctaaagc cctctcggca tataaggata ta
//
LOCUS       DAWBSW010000086         8095 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1824_length_8095_cov_2.600249, whole genome shotgun sequence.
ACCESSION   DAWBSW010000086 DAWBSW010000000
VERSION     DAWBSW010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1824_length_8095_cov_2.600249"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1691..1700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4308..4317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4829..4838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2150 a         1746 c         2011 g         2158 t
ORIGIN      
        1 gaatatagcc gtcactgccg ccgccgacct tgtagccttg gaaatcctcg gttgaagatt
       61 tcgactgacc tactaccgca aagccgccct tgatgcaggc gattcccaga acggaatcat
      121 catcggcacc gccgataagt cttgaccagc aaacctgacc cttggagttg tatctgagca
      181 catatccgtc acgcttgcca taattcattg tataaattcc gtcggctctt ttgtagatag
      241 taaatgtgcc gccgacaaca caaccgccgt cgtccgttac ggcgagggtg ttaaattcgc
      301 ttttgcccga gccttggaga tactcagccc agtcaaggtt gccttttttg ttgagcttga
      361 ggagaacggt gttctctgtt cgaatcttat tgaatctgat tcccgagaaa tccccatcgg
      421 tggacattgt tacacatgtt gcgtaaatat ctccgtcgga attgacagct aaagcggaaa
      481 taatatttga ctttgagcct tgaagaacct ttctccactt aatatttccg tttttatcaa
      541 acttgaaaat aaaagcctta gtgccgccct tttgcgtgcc tgtgaagaat cctgattcgg
      601 agatcgcctt accgcctgca acgaatcctc cgtcaggggt tgcggcaagt gagctgatgt
      661 attcgcctgt tgaggaggcg tcggacggga aaacatattt ccacataaat gttccgtcgg
      721 ctttaagtct gacaataaat gcggagtttg tggatgctgt tttcacaaca tcgccgctcg
      781 ttgccgcagt ctgaccggcc gcaacaattg tgccgtcctt aagctctata acgtcattga
      841 agttaacctg gctgttgccg cccgttatat atttccattc gacctttccg tcggaggaat
      901 atttaacaag tgatgtgtgc tgcattcccc attcgcctga aacgcccttg tagtcgccgt
      961 cctttgacgc tgaaattcct gcggcaacaa atccgccgtc tttgcagccg atgatatctc
     1021 tgaaggcgtc aagtccggaa ccgccgaaaa acttggtgta ttcgcatttt gcttcgtcaa
     1081 cggattcctt tgacggcttt ttgacgccga ccgttgttga tgttgtaatt ttagcggccg
     1141 aagctttggt tgtcggagtt tcgttcgacg aatagtcggg agattcgttc gtctgctcga
     1201 gctcaagctt tccgtccttg tatttgccgg tgtaatagat catatcccca aaagtgtcag
     1261 tctcgttctt agtgtaaaga atgttgcctt ttgagtcttt cttaccggtt gtgtaaattg
     1321 ttttgccgta gttgtccttg agcccggtcg aatatattct gtgaccggcc ttgtcgacaa
     1381 ttcccgttgc gtcggtgacc ttgccgttag ggtctgttgc tcctgttatt gccgtgacct
     1441 cctcggcgcc ctttttgagc tcatcatcaa cgatatctaa atcgtcggga gtctcggaaa
     1501 taagcttgtc gttcttttca cccggagttg cggaatcgcc gcaggcggag aacaaacaaa
     1561 gcgacattgc caaaacaagc aacaaagcaa ttattttata tttgttcatg tgaaatcctc
     1621 ctttaacagg ttggcaaccg cctgtagcaa ataaatcggc agttactttt ttctataata
     1681 tcatactttc nnnnnnnnnn atttttaaaa agcagcaccg caaatgtgct atgttgattc
     1741 aacgcaaccg tcggttgaca gattatacag tcgggtttct tgcctgcaac cgtaaaaaat
     1801 ggtatgctat cggcaagaaa aggagtgatc accatgatct tggcagacaa aattatatcg
     1861 cttcgtaaaa aatcgggctg gtcgcaggag gagcttgcac agcagctcgg agtaacacgt
     1921 cagtcggttt caaaatggga gggcgcacag tccgtgcccg acatggataa aattttacag
     1981 atgagccgcc tgttcggagt ttcaacggat taccttttaa aggacgaaat cgaagcggaa
     2041 gaaaaaataa cgacaagtga ggattctccg ctgagaaggg tcacgatgaa agaggcctcg
     2101 gattatatcg agcttcgcag aattgccgca ccgaaaatgg cattcgcaac attcctttgc
     2161 gtcatatctc ctattgcgct gattttgctt gcaggcttga gcggggtggc aaagttcggt
     2221 ctttcggaaa atcttgcggc aggcatcgga ctttgcgtac ttccggtaat cgttgccgtt
     2281 gccgttgcaa tctttctttc ctgttcgtct cgctcaaaag attaccgttt ccttgaaaat
     2341 gaagcctttg aaaccgagta cggagtttcg ggtatggtca atgagaagaa aaagaatttc
     2401 agcacagcat attcacgctt caatgctgtc ggaacgctgc tttgcatact ttccgttttg
     2461 ccgcttttta ttgctttttg ttttaaagca tcggatatat tttatatcat cggagtttgc
     2521 ctccttttga ttattgcagg tgtcggagta tatttcttcg tcctcggcgg cgttcggcag
     2581 tcctcgttca acaagctgtt ggaggaagag gactataccc gtgacaaaaa atcaacgggt
     2641 aagctgagag gtgcgataac gatctgctat tggctgatcg ttacggcgat cttccttgtg
     2701 ttcacctacg gaccgaacgg caacgctcag ccccaaagcg ggtggataat ctgggcggtt
     2761 gcaggagtaa tttacggcgg acttgcagtc ctgacgaatt ccgttatctg caagaagaaa
     2821 aagtaatttt ccgaaaagag gaaaacaaat gaacgatgta attgaaataa aaaacttgag
     2881 aaaatctttt ggcgaggtca aggccgttca ggatatttcg ttctgcgtaa agcaaggcga
     2941 gatgttcgct ttcctcggtg tgaacggagc aggaaaaagt acaactattt ccataatatg
     3001 cggttatctt gcaaaggacg gcggcagcgt tgaggtctgc ggaaagagta ttgacaattc
     3061 aattgacgat atcaagagaa atatcggcgt agtgtttcag aattccgtgc tggatcagtc
     3121 gctttccgtt tcggataacc tcagaagccg tgccgcattc tatggaataa agggcgccga
     3181 attcaaaaaa agacaagccg agcttgcaaa aatgcttgac tttgaaaagc ttcttaacag
     3241 aacggtcggc aagctttcgg gcggacagcg gcgcaggata gacattgcgc gtgcgcttct
     3301 tcacaagccg cagctcttga ttttggatga accgacaacg ggtctcgacc ctcagacgag
     3361 gaagctgctc ggcgatgtgg tggccgattt gagaaagaag gaaaatctga cggttctgct
     3421 cacgacgcat tacatggagg aagcggcgga tgcagactat gttgttattc tcgacagcgg
     3481 aaaaatcgtt gccgaaggaa ctccgcttga tttgaagaat aaatacacag gcgattttat
     3541 aacaatttac agggcggatg agaacgaggt taaaaagctc ggcgtgccct atgaaataat
     3601 tcgtgatgcg ataaggattt ccgttccgga caccgcggcg gcaacaaggc tgattacgga
     3661 aaacccgaat atgttcactg attatgaggt cacaaaagga aaaatggacg atgtttttct
     3721 taacgtaaca ggaagaaagc tgacgggagg cgatgaaaaa tgacaggtct cggcaatttg
     3781 ataaggcgaa atattcgtct tttcttcaag gataagggaa tgttcttctc ttcgcttata
     3841 actccgatta ttcttcttgt gctttacgtt acttttctcg caaaggtcta tcgggacagc
     3901 tttgcgtcgg ctatgcctca gggaattacg gtggacgaaa agattatcaa tgccgttgtt
     3961 gcttgtcagc tcatttcttc tctgctggct gtcagttgtg ttacggttgc tttctgttca
     4021 aatcttctga tgataaacga taaggttaac ggtgcaattc gcgacctgac ggtttctccc
     4081 gtgcgccgtt caacattggc tctcggttac tttatctcat cggcagcctc gacgctgata
     4141 atcagctttt ccgctctcgc cgtcggactg gtttatgttt acactcaggg ctggtatatg
     4201 acgctcggtg atgtgttgta tcttattctt gatgtttttc tgctgacact gttcggaaca
     4261 gcgctttctt catgcgttaa tttttcgctc acaacaaacg gacaagcnnn nnnnnnntgc
     4321 aggctacggt tttatttgcg gcgcatatat gccgatgtca cagtttggca gcggacttca
     4381 aaaggctctc ctttttcttc cgggtacata cggaacaagc cttatccgaa accatgcgat
     4441 ggcaggcaca ttcagaaaaa tgagagaaat aggatttacg aaagaggcga tagaagaaat
     4501 tacaggtgct gttgattgca ggctgttttt ctttgaacat gaggtcggaa ttccggcaat
     4561 gtatcttatt ctctccggtt caaccattgc actgataatt ttatatgttt tgttgaacag
     4621 ggctttttcc gggagggcga gatgagcaaa tacgagcctc tttggaatta tgttaaagag
     4681 aaggagaatg attttatact gaactatgag caggttgaaa aaattctcgg atttccgata
     4741 gatcattctt tcctgacatt caaaaaggaa ctgacgaatt acggttggca ggtcgggaaa
     4801 atctcgacga aaaacaaaaa cgttaaatnn nnnnnnnngg cagatttttt gcgaataata
     4861 gaaaaataca ttgatttaat catcagtttg ttttattatt attttaaagc caaaaagaat
     4921 ggagatttga ttatggataa atacgcaaga ttcagatatc aaccgtgtat accgatgggc
     4981 gcagacggca gaaagctgac gggctcaccc gagcacaccg cactctcaag aaaagccgca
     5041 ggtgagggta tggttctgct caagaacgat gataacgcat tgccgcttaa aaaagacgaa
     5101 aaggttgcgc ttttcggcaa ggcgacaatt gaatacataa agggcggcgg aggaagcggt
     5161 gacgttttct gcgcctacac ccacaatatt tacgacggct ttgctcagaa agagaaagag
     5221 ggcaaaatca gtgtatatat gccgactgtt gatttctata aggaatacgt aaagaaagaa
     5281 agcaggaaga tccctacccg tgccgaaatc gaaaagactt gggatatagt caatgcaatg
     5341 gatttctgcc ggaaaaagga tgacattgtc tatgatactt ttgcaagcat gcacgttgtc
     5401 gaagcggaag ctcccgatga gcttataagc gctgcggcag aaaacgcaga cacagcaata
     5461 atcacgctca gccgtttctc cgccgaaggc gtcgaccgtc gtgccatcag cggcgactac
     5521 tatctttccg atgcagcaaa gagccttatt gaccgagttt cggcggcatt caaaaaaaca
     5581 atagttgttc ttaactcggg cggagtggtt gactgtgagc acttcgccga gaacgacaag
     5641 gttcagggca ttctctgcgg ctggcagggc ggcatggagg gcggtatggc ggttgccgat
     5701 attctttgcg gtgacgtcaa tccgtcgggc aagctcggcg acacaattcc gaaatcatac
     5761 gactgttatg agaacggaaa gatgttccaa acgggttatg agcaccttga ctatgaggac
     5821 gatatttatg tcggctatcg ctattttgaa acaattccgg gagcggcgga aaaagttcgc
     5881 tacccgttcg gctttggtct ttcttatacg gactttgaga tgagcggcgc tttttgcggt
     5941 gagagcgaag gcaaaattgt tgctgttgtt acggttaaga atatcggcaa agtctccggc
     6001 aaagaggttg tgcagcttta ttacagcgcg cctcaaggaa agctcggaaa gccgtcaaag
     6061 gagcttgcgg catttgcaaa aacaaagctt cttgctccgg gggagagcca aaccatggca
     6121 ctgagcttcg atataaatga catggcaagt tttgacgatc tcggaaaaat ccaaaaatcc
     6181 gcatttgttc ttgaaaaggg aacatataaa ttcagcctcg gaaattctgt cagaaacacg
     6241 agactgctcg actacgaatt tacggcagac gaggatgtta tcgttaagca gtcgaagtca
     6301 cttcttaaac cgtttaagct tgaaaaaaga ctccttgcgg acggaagcta tgaggcactt
     6361 cctcagagcg agcaaagcta tgattcgggt aaaaataact tagccgatgc aaaggctccc
     6421 gacgaagcgg ttatgtttga ttatgtcggt gaaaaaatca gtcttgacga ttttatcaga
     6481 cagttcaccg ttgatgagct tatagatttt gtcggcggac atcagaatca gcccggcgtt
     6541 tgcaatacag gagccttcgg cggtttgaaa cggctcgata ttcctccgat tcctacagcc
     6601 gacggccccg caggtgttcg tctcaacgcc aaaacaggag cacctacaac ggcgtggccg
     6661 tgtgcaacgc ttcttgcctg cacatggaac accgagctta tcgaggaggt cggttcggcg
     6721 ggcggtgccg agctgagaga gaacaatctc ggcgtttggc ttgctcctgc aatgaacatt
     6781 catcgcaatc cgctttgcgg ccgtaacttt gagtattttt ccgaggatcc gcttcttgcc
     6841 ggaaaatgct gtgcggcaga cgtaagggga attcaatcaa gaaaggtcgc agcctcggtc
     6901 aagcacttcg cttgtaataa cagagagtca aaccgatttg aatgtgattc gagagtttcc
     6961 gagcgtgcgc tgagagaaat ctatctccgt ggctttgaga tatgcgttaa ggaagccgac
     7021 ccatggacca ttatgtcctc atataatatt atcaacggct gtcacacatc aacaagctat
     7081 gagcttttga ccgagattct caaaggtgaa tggggcttca agggcatggt tacgaccgac
     7141 tggggcgttc acagccatca ttccgacgag attcttgcgg gcaacgacct caagatgggt
     7201 gagggcgaac cgaatgagct taaagaggca tacgaaaacg gcaaaataac aagagcagac
     7261 cttgaagcct gcgtcagaag gattctcgtg atgacaataa atgttgccga ataatctgtt
     7321 gtaaatgcga aaatgtttat gaatatttta cctgagtaaa acaattctgt ttttttgaaa
     7381 tttcctattg taatttcacc gaaatatatg tataatggta cggtaaattg aatgcggacg
     7441 aaaaaacaat gtattttgtt gcgtctgcgg taaatccgtg cgcattcggc acacggtgga
     7501 ggagttagga acatgcttgg taaatatgtt cgtatcaagg tgactcatcc gatccactct
     7561 tacagcaatg aacagggctt tacctatgaa ctgaactacg gcgaggtcga gggggctaag
     7621 cagttcaacg cttctgttaa aggcgcattc atcatgggta tcaatcatcc cgtgcgaagt
     7681 tttgacggaa gggtagtcgg catcgtcagg tatgacggca gtaatgaaac agattgggtt
     7741 gtagcgccta aaagtacaag gttcatcaac attgacatcg caaacggcat tgactttgcg
     7801 gtgaagaaca aaaatcacac actggagtgc ctttatgagc gttcctgcag agcggttgtt
     7861 tacaggaaca tcggcggaat gattcgtttt ctgcttatca agaacagacg aagcgcccat
     7921 tggggttttc cgaagggtca cgttgagcag ggcgaaaacg accatcagac ggcttaccgc
     7981 gaggtgcttg aggagacggg aatcagaatt aacattcagc ccgatttcgt cacaaaaagt
     8041 cagtacacca ttcagggcaa ggttgagaaa aacgtaaata tttttcttgc ctcga
//
LOCUS       DAWBSW010000087         8061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1834_length_8061_cov_2.925056, whole genome shotgun sequence.
ACCESSION   DAWBSW010000087 DAWBSW010000000
VERSION     DAWBSW010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1834_length_8061_cov_2.925056"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5098..5107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6012..6021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2229 a         1770 c         1660 g         2382 t
ORIGIN      
        1 tgtattttgc tgttgtatcg gctgcaccgt tattgtattt atacgttatt gtataggtat
       61 ttacagtcca ctgtgcagta agagttacat taccatactt gcctgacgga atgcttgtgc
      121 ctgtgtaaac atcattgaga gtccaattgc ttacatctgc cttttctgtt accttccagc
      181 ccgagaaggt gtaacctgtt ctcgtcggcg tcgggagaga aatagaatca ttgattgtga
      241 aggtcttgga agtcggagtt acgcttccgc cgttgggagc aaaagtaatg gtataactgt
      301 ttacatccca ctgagaccaa agctgagtgt tgcttgaagg catttttgtg ctttcggtat
      361 aagttgtacc cgtaccgttc ttagctgtgt tccagcccgt gaatgtataa ccggatcttt
      421 cgatatcggc aagtgaacct acggtctcac ccggaagctt aaccttgttt atcggatcgg
      481 ccggagtgac gtcatccgca acacctcccg gacttgtagt atcctgaaca tagatgttgc
      541 tgaattcggc cgtatcaccg ttggcagaat catttattcc aaagcgaagt gctccgtaat
      601 atctgcctgt ttgcactgta taattaaaat caattgttga ctcggcaccg cttgcgacgg
      661 taacgcctgc actctttctg tttccgtcag tgtctgtgct gttaagatta gaatcggcaa
      721 agcagaagat ataaactgcc gcatatttag cgcttgaaga tgtgttctta accgtagtcc
      781 ttattctgta ggtatggccc ggtgttacgg caaaagcacc agatttaatt ccataccact
      841 gacctgtata tgcatcggag ccttttgcat taagtctcag cttgtttgag ccgtaatcaa
      901 tgtcgaacgt tgaattgttt gcgccggagg caatatccga agcagtgata ttgtcaaaac
      961 ggaactcatt atcaaacatc agctcgggag caacagtcca ctttgcataa agccttaaat
     1021 tgctgtttcc gacagttgat gtcgaagtgt atttcgttcc cgtaccgttg ccggttgagt
     1081 cctggttatt ataccaaccg tcaaaagcgt aacccattct tgatatactt gcaacattgc
     1141 cgatttgtga gcctgaaatc aagctcagat ttgcatgtgg tgttgcatcg gtaacatcat
     1201 ccttgaatcc gcccggagtt gtgatatcct gaatatagat atcgctgaat gaagcggtaa
     1261 tacttgccgt tctgagtccg agacgaagcg tcatatagct tgcatccgac ggaatcttaa
     1321 attcactcgt caaggttccc tcggcatttg ccgcaaggct gattgaaggt cttgaataat
     1381 aaggtgatgt gcccaccgac ggattagtgc gattggcgtc tctaaaggca aatgccatgt
     1441 aatcaacagt accggccgtg ctgcccatct taactttcat acttattctg taagtatggt
     1501 tcggaagaac attcatgtaa tatgaacctg ttccgttgat attacttgtt gaataatccg
     1561 tgcttgttcc cgggtttgtg attgtggcag tattggtagc catatcgaag ctgtgagctc
     1621 cgctgaatgt agccttcaat gcccactggt cgaaatcgaa ttcattatca aaaagaacat
     1681 cgggatttaa aatgtaatag aatgtatatg aataattaac gttctttgca ttgccgaata
     1741 caagagtttc gttctttgac ttggaaacag gatcggtact gttaaccata ttattataat
     1801 cggttgcgga gaaggcgtct ccgttggacg atgctccgtc ttttatataa ccgacatatt
     1861 tatagccctc atactgctcg gcagagaact caaccttatc gtatgcctta aaggtcttgg
     1921 agatcgaagc tgtgccgtca gaagctgcct cggcaatagc tgcaaccgtg aaagtgccgt
     1981 tggaatttgc aacaagtccg acatttttct gtgttgcggt gtagttgttt gtgtttctcg
     2041 gagcagttcc tctgtttgca ttgagaagaa catcaacagc gtctttaagt gtctttgcaa
     2101 gaccgctgcc cgctacattg ccctcaggga aaatgccgaa atttgccgtt ccgtcaactt
     2161 ttgtcagtgc atcctgcgca ttggcaagag cggttgtata attagtccac tgtgttgaac
     2221 cgttgaagta ttctgcctga agcgcatagc tgtactttgc ggcatactcg actgccttac
     2281 ggagaccgga gcggtcggaa gcagaaacgt ctaccgctga atatgcgttt gtccactcac
     2341 ctttatcgtc tttattgtta gtatacgcat aaagaataaa tgatgcaacg ccgcttgtag
     2401 aaaagcttga aagatctgtt ccgctgatat tatatgcctg cggttttgtt gatgaatatg
     2461 tcgttttatc gcccttaaca acagtttgtt taatagcact gcctgatgtt cttgagttaa
     2521 cagactttgt cattatctcg gcatatctgc acatattact gtcagagtca tggtttgtaa
     2581 acatggcgaa attaggtatt tgtccgaaat tcgaaaaacg acttgtatcc acgtataacg
     2641 aaccgatacc ggatacggca accataccgc cattacccga atctctgtaa acatatgaca
     2701 ttccgtcaaa gcccgaggca gtgctgtaaa cttttttatt gttataagga tcagttctgt
     2761 taaaccaacg ataacctgta ccgttcgtat cgtaagtagg tttagttaaa atgggttttg
     2821 ggttgtatcc tccgccgtag ccgtttttgg tattactgtg atcagaagtc gtatttgaat
     2881 tggttgcata ataaatgttc atgtttttat aaacattttc ttttaaacca tgaatacccc
     2941 aaataatgct tgtagcagat tcacactcac ctgaactgtc tcgtccgaga gccgcagcag
     3001 ctactgaacc gacatacgga ctgtaaacat acgatttagc cgtaacagtc ttttcaatgt
     3061 tatcctcaac atacgttgct gtccatttga tatagcttga cttcgcctct gaaaccgaac
     3121 cgccggaaat gtttgctgtc gtaacggcag actgcgatgt gtttacatat gagttaaagg
     3181 aagtaacatt tcctccgctc agagaaacac tcgacgcacc gctttgggtg atcttaacct
     3241 tgcttgcatt tttataatag aaatatatat tacctgttgc atctttgtta gcactgttta
     3301 cggtaggtgt gcaaacgccg ttgctcaaag ataccgatga catatccgaa taatactgga
     3361 atgtcgtttt gcccggtgta agataaatta cctcgggaac atagaaataa acataattat
     3421 ccgcagtcgt agtcgaatta acgatagagg tttctatcgc cttaaccgaa cccgaagccg
     3481 ttccgctcag agatgtagtc gtcttggaat aaagctgcgg attgcaaaga gtttccgcta
     3541 ccgacttaag ctccgtataa tatgccgaat agccgcttga atatgttttg taatcaatat
     3601 gggagtgtga ggcatcgtta taaacagtac gaagcgtctg cttattgacc gtctcggtga
     3661 aaagtccgtt atagtgatga acaccgatac tctcggtata tcccacgaaa tgcttatatc
     3721 cgttgaagaa ccacgtatca aatgtaacga gtccgtctat aatcgaaccg ttaaacttat
     3781 aaagacccct tgcaacagaa tagctgtcgt gatctttgta gttagaagta tcaacatcgc
     3841 aataaatcgt tccgtcatat gcaatgcttg ttgaactgcc cttgttatat ctcttaacgc
     3901 tcttgataga gttaagcttg ttaccgttac cgtcctggtc atgatagaac tgtgcataac
     3961 ctgcactgag gtaaggaatc tgattatagt ttgtatatct ggaggtatca acgacaatgt
     4021 aagccatacc gagggtaatt cccgatccga tcactccgtc gcccgtgttg gtaatagata
     4081 cattacctgc aacattaccc gaatagcttg atggaacaga gatacttgtc gtatcataat
     4141 aaagctcatt ttgacctctt gtcggcctgt tataaacaaa ggtattataa ggagtctggg
     4201 aagacgagaa gttgttttcg tttaatgcct gtccgtcgcc gcacggaatg gtatagttat
     4261 tctcattctg agcacgtccg acaaacgata caagcggagc tgccataagc ttattcgccg
     4321 ttgtaccgag gaattgagac tttctgttac cgcccgaata cttcatcgca cccgtgatga
     4381 aagaccatga atggttctcg gtcgggttac tacccgaagc aatatatctc tgagaggtcg
     4441 aagtgccggc ctgatcaaga agcggaacat aaacgcccgt atacataaag gcaaaatggt
     4501 atgctccgtc aaccttgtaa cggaataccc actgaatgta atatgtagtt cccgttgctg
     4561 cgcccgtaag cgaacactgg ctcgcattaa gtgtaacgga aattgctccg cttgcactgc
     4621 ttgcaatcga gctgaacgag gttgaagttg tcgcattata agcaacctta ttctgcgtaa
     4681 cagtaacatt gttgatttta agcgttcccg aaaaatcaac atcgttttcg ccgttcttgt
     4741 aatagacttt gttgacgccg atggcaatct ctgttgcgcc cttgtagtca aattcaagtc
     4801 tcgatgttgc gtcattcgtg cttatcgcct tgccggtcgc aggatcatag ttgctgaaat
     4861 actgaaacgc actgctgccg ggcttgagat aagcaatctc gggcgcatac ataaactggc
     4921 tcgagagtga acccttcatc ttgttgtttt ctgttacggt gaccatcgcg ttcgatatca
     4981 cggaaccgat atcgaagaag cagaaggtcg tcagaagcat agtgagacac aggattacgc
     5041 tcaatgtgct ccttccgaat ttggtaaatt tttttatcat tcttttacct cccatacnnn
     5101 nnnnnnnact tggtaattta ctacattttt caagttgtca atttgtgaaa actgtcgcaa
     5161 aaatatactt tgccaacttt aatgggaatt ttagcataca taattatagc gtttcaacaa
     5221 tgttcattaa catacattta ttttcaatta tttgtatata aattttcaaa ttattaaatt
     5281 atatttactg tataattcga cataaaaaca atatttttgc cgcaatttag ttttatataa
     5341 gaactaacat tttttaccat aattgcataa ttttcaatac ggaaaattgt ttaattctta
     5401 tataatatat taactgtagc aatacagtat tgtccgcaag gcatagatga caaaaatgca
     5461 aaaatgtaaa aaataaaagc tttatatgcc gtcgattaca gatgcaacgt caagatcttc
     5521 tctttgacat caattcgcca ttaattcttc ccgacggttc tttttgagtt aaaaagtaaa
     5581 aaaagacggc ttcttaaaag aaaagccatc tttttatcgg aatcataatg ctatgtactc
     5641 atgttaaacg cacttatatt cttttgaatt tataacgacc gcgataccgt tttcagcctg
     5701 accgtcaaaa tcgaagctcg gcataaaatc agacatatct gtatttttca tgttcttttc
     5761 aaagcagaaa tcaacggatt tgccgttaac caaaatctta acctgactct ttctcaggaa
     5821 aaagaacacg ggcgacatca gctttgcctt gccgttttcg gcattgagct tgatattctt
     5881 tgccgagaga agctctctgc cgttcaggac aatgcttatt ttaccttcat cgcaaagcac
     5941 aatcggatga ctgttcgggc ggcgcataag ccaataaaag cgctgcagct ccggcacgga
     6001 cggatgcaga annnnnnnnn ngaacgattg tgtcctcctc aattctcgca agcagtcggt
     6061 caattcgcat atcagccttt tcccattcct cgccgtcgag atgaacaggt aaaaatttcc
     6121 cgtccttgag cttccatcgg aaatcgttga acactatgcc ctgataatat gtcggagcaa
     6181 agcgtccgaa gctgtcaaaa agctgctcgt aaaagtccat tgacgtgtca aatatatttt
     6241 cgggtgaaaa tttcgttgaa acaatgctgt ccatgttgtc acggttatat atatactcgc
     6301 ccttgtcgca aaaaccgatt ttcattttcg ctctgagtac ctcacagcag tatttctcgt
     6361 cctcatggcg gaaattcggc gttgtgtcaa aaagaagatt gtccgctccc atgttcttga
     6421 tgcaaatatt tacatttgtc tgcgtgataa acctgttctc cgggtcgagc aaatcataaa
     6481 caccggtttt cagaagcgtt cgatatctgt aatgtgtaat aacgggaacg ccgttaaagt
     6541 attgaattat tttatatgtt acaagatcaa cctcgtcgta aaccgtatcg aaaaaatccg
     6601 tcacggattt taccgtgtcc gctctgagct tgtcatcgga atcaagaaag aaaatatact
     6661 ttccgttcgc cctcgtgaga gcgtaatttc tcgttttcga aagcccctca ttttccttgg
     6721 aataaagcac aacattttca tatttatcgg caaagccctt gcaaatcttt tcgctgttat
     6781 ccgtcgagcc gtcgttaacg agaaggactt cgatattctc aaagccgatc gtctgctcgg
     6841 tcagagaagc aagacagctt tcgacaaatt tttcaacatt atagacggga ataatcaccg
     6901 tcgccttgta ttcaggcatt gaagtcacct ccgagcctgc tcttgaagaa cgtgcagagc
     6961 ttgtccctaa gagcgccggg tttcatgtga tatgctttgt aatagaggta ggcaaaaagg
     7021 cactgtttga gaacgacctt gccaaatttt ccgaaataaa agctgacaac cgcatccttt
     7081 gcctttatag gcataactac ctcgctgtat tttgtttccg ttccgagcct gcgaaaatcc
     7141 tcaacactta tatcggtctt aagaagctcg gcatcccagc cgtaagcctc cttgaaccat
     7201 gcgcaaacgg catagctgcc catgaacggg agcaaaacat ttttgagcat ttcaaagctt
     7261 tccttctgtt cgcacgaccc gaaaaccgac atcacgttgc gaatataaaa aagcgtcaca
     7321 cggtcgctcg ccgtctgaga aaaaagccac gaataatttt caaggcttgc aatgtcctcg
     7381 gcatcggccg aagcctcagg cagccgcgtg atattaaaaa ggctttcgtg aatcggcgtg
     7441 atctcatcat atcgcgcata gtcggctatc atgttcataa ttgaaaatgc cgtgcctcct
     7501 acaggcagaa tttgattttt ttgcacggtt tcggcattca taaggcagca gaaatagccg
     7561 ttcttcgatg caatctcatc aaataaaaag tcagcgaaaa aaaggttgcc gtctgccgag
     7621 gcaccgttaa acacgcatac acctcttgca agtaatgcgg caaaagcatc gtcagcatag
     7681 gttataccga catcggaaaa aagaatataa ccgccctgcg cgtcggagat cgcctttttc
     7741 aaagcaaccg ccttatcctg cgccggaaaa accttgacct tggcaagaat atcttcaccg
     7801 agagaggcaa gcctttcggg atcaatcctg tcacagataa gaacctcgac atcaactccc
     7861 tgcgaaaaaa cattgtccaa ccagcctgcc agtctgccct catcggacac ctgaggcaga
     7921 attacactta tatatgattt atccatttaa atatcccctt gcatgaaaaa taatcaaaat
     7981 attataaaaa taattctata aataatattg actaaatatc atttagtgca tataataata
     8041 ttagaaaaaa agaaaggagt t
//
LOCUS       DAWBSW010000088         7935 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1861_length_7935_cov_2.590355, whole genome shotgun sequence.
ACCESSION   DAWBSW010000088 DAWBSW010000000
VERSION     DAWBSW010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_7935_cov_2.590355"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6379..6440
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6856..6865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2115 a         1893 c         1951 g         1904 t
ORIGIN      
        1 caagcagaag acggcatacg agataaaaga cattgttctt tgcgttcggt gcggtgttgg
       61 ttgtgctttt cggtacactg cttactgtcc tttataagga ctttacggat tttctttcct
      121 ttgacagcct gataaaggcg ttgaccgaca cgggatatgc gaccgatgag gacattggca
      181 gatttaccgc cctgcccgtt atctcgcaga gatttttgcc cggctttttc agaaagctgt
      241 tcggcttagg cctcggtaac tgcgacagct cgtcgctcag tatgttcaat acgccgtttt
      301 tcgaatctca tcaaacggtt cactattcgt atttttcgta tgcatttttg ttcttggaaa
      361 caggctttgt cggactggca ttgtatgctt cgtttttcgt cgcttcgttt ttcgtttcaa
      421 gaaagctgaa aaagctggaa atggcagacg aatttgcttg tcagatgagc attatacttt
      481 cggtgataag cctgatactt ttggtttaca attcctcttt gcgcatggag atcggattta
      541 tgctgttctt tgttttggca ttgccgatta tttccgccaa tgagcaaaga gaaagtgatg
      601 atgtttacat ataatcgttt ggaaaaatgc aaacaaagga ggcaaaaacg atgagaagag
      661 tgtcaaaaag aattatatct ctctttttgg tattagtgat gcttctcgcc atgatacctg
      721 ccggtactgt tttggcaagt gccgcgtcgg gttcgctgga catgaccgcc gaaggactta
      781 ccgcaactta tgcgggtgac ggcacatgga ccggcggcgg aaatgtggcg cacggcactg
      841 ttaccggtaa gggctcgctt ttcaagcagt cgcagagcgg ctctcttacc ttcactaaca
      901 ccagaagtca agccggatat atttcttttg attacaaaat cacagagaac accggatcgg
      961 ttaccattgc cggtaatacc gttacgggca gcggctctta tgcgccgtcc gcagctttgg
     1021 gcagcggtga aacggttcag gttgttatca aatccggtaa gggtaacgac aaaacaacgt
     1081 cgattgagct tacgaatatc aatcttgttg ttgacgtcag tgccacgacc acctttaaag
     1141 caccgattaa cggttcctat accgtcaacg gtgagtctat cacaaaggac acagccaaaa
     1201 cgcagtcctc aacggtagca tataagcttg ttgcaaagcc tgcaacgggt tataacttta
     1261 tcggttggta cagctcggca atcaacggat atttcgatta caacagtaca acaactctga
     1321 atgttgaaag cgataccgtt gtttaccctg tttttgtcag ctcgtcaatg gctgtatttc
     1381 aggtcggctt caatctttac accgatttga ataaggcaaa tgccgccgca gaggcggatt
     1441 cgagcaagca gattgtgctc tcgcacagcg gtattcttgc cgccggcaca tacaagattt
     1501 caaagggagt taatctgttg attcccttcg attctgccaa tacggccacc ttcggcgacg
     1561 agcccgaatg cacaatgaaa tatgctgccc cgaccatgta ctcatgtctt accatggcgg
     1621 acggtgcggt tatcaactgc tacggtaata tcaatgtcaa cgcaacccag cttgcttcgt
     1681 caacaagaac cgcaggcaga gtctgcggcg cttacggtgc aattcagatg acccccggct
     1741 ctcagattaa actgaagagc ggttcggcac tctatgccta cggctacatc ggcggcgaag
     1801 gacttgtttg gggcgaaagc ggaagcaagg tatatcagtt catgcagatt gaggactggc
     1861 gaggaggtaa cgattcctct tcattgcttt cttcattgaa aaacaactcg ttccttttct
     1921 ctcagtatta tcttcagaac gttgaggccg agcttcgtat agacagcggt tgcaccatgt
     1981 acggtaccgc cgcaatcgcc gcaggcttgg gcagctcgaa atcaccgagc caggcctgcg
     2041 ccgcaatcgt cggcaaggat gagggtatgt tccatatcac aagcggttat gccacactga
     2101 agtatgacgc aacgacagac agaatgacgc tcgatttcta cggtgacctt gtaacggacg
     2161 ctatcagtat gtcgattaag ctcactgttg tttcttcgtc aatgaatacc gccgattaca
     2221 ttctcgctct tccgatgaac tataccataa atattaaatc gggcagtaat gttacatttg
     2281 ctcagaagtt taagcttctt cccggtacgg ctatcaatgt tgaaaagggt gcgacggcaa
     2341 tcgtttcctc gggcggcgca gtttacctct atgacgttga cgactggatg tcgggcacat
     2401 acacttacaa taagaacatt ttccagttac cctatgtatt tgcgaaaaag ggcgctcccg
     2461 taactcgcag tgtaaaggaa aattccgttc tcagggttga cggaacgttg atcgcaaacg
     2521 gcccgatata ttcaaccaaa tccgtcacaa acggcggcga tgccgttatc aagggtaagg
     2581 gcgtgtatgt ttcgaatgtt cacggctcga cagaccttaa agaggtcacc ggctcgaaca
     2641 ccacgcttaa aaccatcgcc tgcgttcccg tcaggggcac gatcgcagga cacagcggat
     2701 acaacagctt tgctatcggc acataccgct cgttgaacgg aaactgctgg tatcagagag
     2761 cagttacggc taacggcttt actgtcgttt cgggcggcgt tcaggacggc gtaaacgttt
     2821 atgttgccga cacaaaggac gcaaccgtta cgctcgttct cacaaccgaa cagccgtgcg
     2881 ttacgcttcc cacaaacgct aaggttgccg taagcggcaa taaatataca ttgaccgaga
     2941 ttaacagcaa tgttacgatc gtcgcaaaac agcataatcc ggttgtttct gttccggcaa
     3001 aggctcccga ctgtcttaac ggcggctata cggccgaaga aaaatgctcg gtctgcggca
     3061 cggtcgtaag cccctcggta agtgttccgg caacgggaca caaggaggag attatccccg
     3121 gaaaagaggc cacatgtacg gaaaacggct tgaccgaggg caagaagtgc tctgtttgcg
     3181 gtgagatcat cgtcgctcag gaggtcatta aggcacacgg ccattccgaa aaaaccgttc
     3241 ccggaaaagc tccgacctgt acggagaacg gcctgaccgc cggcaaggtt tgctcggtct
     3301 gcggtgccgt tatagaaaag caggagatta ttccggctac cggccatacc tatacgacgg
     3361 ttgttaccgc tccgacttgc gaagaggacg gatacaccac ccatacctgt gcctgcggtt
     3421 acagctatgt tgacaatatc gttaaagcaa cgggacacag ctatacaatc acggtggacg
     3481 gcacatacaa ggctccgacc tgtaccgagg atggaaagga agcggatctt aaatgcgcaa
     3541 agtgcggcgc cgttaaaaag ggtgcggtta ttcctgccaa gggtcacgat gaggtgattg
     3601 atgcggcggt tgagcctacc tgcaccaagg acggtcttac agagggtcgt cactgcaaaa
     3661 cctgtaacga ggttatcgtt gcgcagaagg ttgttaaggc gctcggacat gattatgttg
     3721 cggttgtaac gcctccgaca tgcacacagg acggctacac gacacacacc tgctcacgct
     3781 gtcatgacag ctatgttgat tcgcacgttg acagcacggg caccgagcac agctacgatt
     3841 caacaaaggt ttacgagccg acctgtaccg aggacggcta caccgttaaa atatgttcaa
     3901 tttgcggaca tgagttgaag ctggaggaca cgatcgttcc ggcatacggc cacaagttta
     3961 catacgaggt tgttgagccg acctgcacaa cgggcggtta tacggttcgc aaatgtacgg
     4021 tttgcggata cagcgacacg gtcgataagg ttgacgcctt gggtcatgac tttacctcgg
     4081 ttgtaaccga gccgacctgt accgaaaagg gatatacaac gctcacatgt aaacgttgta
     4141 accatacgga aattcccgag gagtcgtatg taccggcact cggacacaaa tccgtcggtg
     4201 ttgtaacaac tccggcaacc tgcaccgaaa acggtgtcat gacatacacc tgtcattgcg
     4261 gcgacagcta caccgagatt atcccggcga caaatcacag ctatgacggg ggcgttgtaa
     4321 ccgctccgac ctgtcatgag cgcggttaca cgacctatac ttgtaaaaaa tgcggacaca
     4381 catatcaggg caactttgtc gaagttctcg gctgtgaatt cggcgattgg gtaatcgtca
     4441 agaatgcaac ctgcaccgag aacggactta agaagagaac ctgtattcgc ggtgaccgct
     4501 atgaaacaga ggttattccg gctaccggcc acagctatac ggcggttgtt acggaaccga
     4561 cctgcaccga aagaggatat acaacctata cctgtcactg cggtcacagc tatgtatccg
     4621 actatacgga cgcaagcgga cataagcttg taattgatcc cgccgttgca cctacctgca
     4681 ccgaatcggg acttaccgag ggtagtcact gctccgtttg cggcgaaatt atcgttgaac
     4741 gtcagacggt tgcggcactc ggacattcct tcggaaaatg gactgtatcc aaggtggcaa
     4801 cctgtaccga aaagggtgaa gaggaacgtt attgcaccgt ttgcggtatc agagaaacaa
     4861 gagagcttgc aatgcttccg catactccgg taattgatgc ggcggtagct ccgacatatt
     4921 catcaacagg acttacagag ggcagtcatt gctcaagctg taacgaaatc cttaaaaagc
     4981 aagaggttgt tgccaagctc aagcttaact gggatacctt taagcttgca atgtctcagc
     5041 ttgagggcta tgcgagcgac tacgccaaga acaaccctgg caaggatccc acaaagctta
     5101 tgctcaactt catccgtacc ggtatcgacc gttacaacga tgacgattgg gtaacaatgg
     5161 caggtgccga ggaaaccgtt ttcgtcaacg aggttatcgc aaaggacgaa atcaacggca
     5221 ctatcgctta tgcgcttcgt gagattgatt atcttgaaat aacaatgccg aacggcgaaa
     5281 aaatggtatt cgaccatctg ttcggttcgt tgaatatttc aagcaataag aactatactc
     5341 agtcaaacac agacttcggc agctgggcag gcgatatttg cgacttgatg gaatattcaa
     5401 atccgagagt aaaagcaaca gagcttgacg caatgaccga agaaatcaaa acaaaatatt
     5461 tcggcgttga cgatgttgag gaaagcggct tcggctatga cgatatcaga accgaccttg
     5521 atgccttcta tatcgtttac caaatcacat cgggcgcttc atcgctcagc gagatttttg
     5581 aaagctacta taacgaggag ctttcgatgc gttcaagagc ggcgttcttc ctgaacaacc
     5641 gcttcccggg cagcctgaca aatgccgagg ttcgtcagtc gattttcacg acttacagag
     5701 atcacctttt gattcagctt cttgaatcgg gcaggggact ttcgggcctt gataccctca
     5761 gagaggcttg ctgttacgca tttgccgatt atctttataa tctggcaaag gacgatctgg
     5821 tagctcccga gaaacccgat aagccgacag atgacccgaa caagccggat atttacagcg
     5881 tattctcttc gaaggattcg atgcttgctc cgggtataac gcagaacatc agctatgctg
     5941 tcgattcgaa cggcgagcag atggtatact atatgtcaaa gtcggacatc aacagaagcg
     6001 atgtcaatat ctacgcaaac tatgcaaaca atgacgcttc ttcatgggca ttgtcaactg
     6061 tcagcgagca gatggccgcc gctcagaaaa agcattccaa ccccaacgac cccgataact
     6121 atattgaaaa ttacaatgcg gttgtcggca caaacgccaa cttcttcaac atgggtacag
     6181 gtcagccgct cggcttgctt gttatgaacg gcaaggagta tacccccatt tacacgaagg
     6241 aaagagaata taatttcttc gctatcctca aggacggcac accggttatc ggagaagcct
     6301 cggaatataa caaatacaag aacaatattc aggaggctgt cggcggtgcg gaaatccttg
     6361 ttaaggacgg tgtaaatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn aacaagcgaa aagatgccga gatgctgcgt cggtattacg
     6481 gcggacggac agattataaa tcttgtactt gacggacgtc agtatccgta ttcggtcggc
     6541 gcaacgtatt atgaggttgc acagattctc attgacgcag gctgtgtgga cgctatggag
     6601 cttgacggcg gcggatccgc aacctatgac gcaaagcagg agggctcgga cgaggtaacc
     6661 gttgttaacc gcccgtgcga cagcgtggag cgttccgtaa gctcatcact tgtaatcgtt
     6721 tccacggctg taacttcaaa ggaatttgac cacgctatcg ttaacaccgc aactgactac
     6781 ctgaccgtag gttcttcctt cgatgtttcg ctgacaggcg tcggtgcggc aggaaatatg
     6841 accgagattc cgaagnnnnn nnnnncactc cgactttccg atgaaacagt cggcactctc
     6901 aacggtaata aatttaccgc cgttgcaaag ggcaaggtcg atattcaggt tgtggttgac
     6961 ggaaaggttg tcggaagcaa aacaatcgag gttatcacaa gacccacggc tctcagcttt
     7021 acaaaggata atatcaacgt gatttacggc gttgctgaag agcttccgct tgttgcaaca
     7081 tacaagaaca atcccgtaac gttcaacacg aacgatatca tctttgaaat gaccaatgat
     7141 ctggcaggtg taatgaacgg cttctcgttc accggtaacg aagcttcggg cgttcgtaac
     7201 gttaccgtaa ccgctaaggt caagaccgat gtcaatgtta gcgccaatat ttccctgcgc
     7261 ctttacaagg cagacgaaag cattttcgat ttcgaaaagg caacggcagg taacgagtcc
     7321 ctcgcatgga acagatctgt ttcgaactct tatacaatcg acaacaagct ttactataag
     7381 gtagatacaa acaaggattc tgtcgcttcg tatgtatttg ccattgatat gaaatcgatt
     7441 accgcagctg tacgcttaca gcccttgatg gaatatctca acggctttgc tggcaacgtc
     7501 ggtgaaaatg ctaccccatg ggattatctt ctcgctttgg gcggacgtgt aagcgacctt
     7561 acaaatgtta ccgttacggc gaaattcccc gagggagtag atgtcgatgt tgaaaatctg
     7621 aggtttgtaa acgacttctt caagatctcg tcatacaaat acgacgaagc cacgcatacg
     7681 cttacaatcg tatgcaagtg gacaagacag actgagggta tcgatccgtc aacggcaaac
     7741 tcgatcggta ttctctccgg tgttaagatt acaccgaaga agaacgccca aaaggataag
     7801 gacggattga tcaatattga tgtaacgggt aatgtctctt acgatattta tcttgataca
     7861 acacagcttt acaagttcgc aaaggatccg gaaaatcaga agaaatacgg catttatgag
     7921 tatatcaatc cgaat
//
LOCUS       DAWBSW010000089         7930 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1863_length_7930_cov_2.830222, whole genome shotgun sequence.
ACCESSION   DAWBSW010000089 DAWBSW010000000
VERSION     DAWBSW010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1863_length_7930_cov_2.830222"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1470..1577
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1835..1844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6396..6405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1993 a         1971 c         1537 g         2301 t
ORIGIN      
        1 acgcaaaaga aaccgctata agagtcaggg ccgatgccac agctaatatt aagtttttaa
       61 taaatgtaaa cttttttttc attgtaacgt caacccttta attatttacc tataacgctc
      121 caaacggatc tgagacccca atcagtctta acctctttga tttcaacagt aatgtcaacg
      181 ctgtcgcttt cgtttacttc aataagcttg ccttcattag caacatccgt aacgttaagc
      241 tcttttccgt cagaatccac agctttaatt tcatatccgt agccctgtgg aagactgagg
      301 tcaatatata ttccgtctgc aacatatacg ctgttaacgg ccttcgcctg agcaccgacc
      361 ttcgtgaaaa agctgaatgc ttcggcattg ttatcggcct ttgttttaag cataacaccg
      421 atgagcttgc cgttaagctt gttcgtttta tctactattg aatagccgat atctgtcaag
      481 ctaccgctcg ccggatttga atatcttgca attatattat ccgttgaagc gtagtctttg
      541 ttcttcataa gtgtaataac aagacccttt gatctgtcca cgcttacgga attagaattt
      601 acttccgacg gaaccttgat atcggtataa ttcattgcag atccgatatt tgatccggac
      661 atatatttta cagcaagagc agcaaattca accgcagttg cgaacttctc cgagctttcg
      721 gttgacttga gagtcgccgg aacagcatat ccggatttcg tgcttgcaaa accgattatt
      781 gaagcttctt caagagcctt tgttgtcgaa gccttcatca tgcctgttgc gccctcgacc
      841 gctgcaatgt cattctttac cgtgcaactg tcaaagagaa cattctctgc cgaagaagtc
      901 tcggcaaatc tgcctgcaat atttccggct ttcttgtcaa gtgagttcgt catatttccg
      961 aaaacggagt tgctgagtgc agtataatct gcttcgccga caagtccgcc tgcatatccg
     1021 ccggctgata catacgccgt tgacaccgta ccgtttatct gagaattgct tgatgagccg
     1081 accaatccgc ctgcagcaag aagcgacgag ccgcttactc tgccggaggt gatacatttt
     1141 tctatctttg tgccgtttgt tgcacgtcct gcaattatac ctgcgtcctg aacctttccg
     1201 aacgtattga catcggcaat tacaacgcag ttttcaatcg ttgctccgtt cagctcgccc
     1261 gccaaagcac cgactgttgt tgatgattta gaatcaatat caaatcccac aaagccgata
     1321 ttctttactg ttccgctgac gcttgcaaac aaagactctc cgctgaaatc ataagcaaca
     1381 tgatttgcac cgtcaagaat gcccgcaaaa ctgtctgcgg atttaacacc gctgaggtcg
     1441 atatcatttg ttaatgtata ataaacatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnngta cggatcggac tttgtacccg agcccgcaag aagtgaggaa
     1621 taagcaaccg gaattgctgt ttcaagaccg tttgaatacg taaatacaag cttagcaccg
     1681 tcaacagcgc cctttgcact gactgaagac gttgacgaat cataagtcaa tacaccctta
     1741 ggtttcgaag aaactgaggc gagctcaaac ttatgtccga gaagctcaaa caacttgtag
     1801 ttaccgttac ctgccgcgat tataatatca ctttnnnnnn nnnncgagaa cagtattttc
     1861 gccgtcaagc gtaataaatg atttttcggt tcctccgaga acacatctca ttgcgctgag
     1921 atcaacggcc atgtatccag agcactgata agaattaagc tcttgcgtac cgaaaatgct
     1981 ctcgttctga tatgatgaat aatagacatt tgacaagatt tttgcattat cattatcaga
     2041 gaatgttccc ttatccgcct ttgcaataac tgccgcagaa atttcaccct cagccttagc
     2101 ggctataacg gtgctctcaa caagagcctg ctcggttttt gcaatatatg tcgaatttga
     2161 tccaacctct gcaattacgc ctgctgcaag cttttcgcct gagacgcttc cgctgatgaa
     2221 acagttcttc acaaagctgt tattcggatt ttcaagcacg ccgatcattc cgccggctgt
     2281 tttagcggaa acttttccgt ctaatacaac gttttcaatc tgtgcttttg ctgagctgcc
     2341 taccgcaccg cctgcaattg ttccggcttt aacggtaacg ctgttcattt gaatatcctt
     2401 aagctctccg ctgaaaattc ccgcaacacc gcctgcggtc tcaaccgttg ccgaggtcgc
     2461 attaagcgtt gttgaagcat ttgattggtt tattgaaata ttttctgctg tcagtccttc
     2521 ggcttttccg gcaataatac ctgccgtact tccgaattca cttgacattg cagttgaatt
     2581 attgaccgtt acatttttaa taacggtatt ttttgcaatg ccgactacaa cgcctgcaaa
     2641 attaagacct gttacacttg cgttgtttat tacaatatcg gagataacgg caccgtccgt
     2701 cgctccgaac agtccgccga gaacattgcc tgaaagcatc aatccgttga ttgaatggat
     2761 ctttccgttg taaagacccg taaagttgcc cttgaagccg ctttcagcac tgccgattgt
     2821 tacaacgccg tttcctacat tatagaatcc gccgccgaaa tcaaagtcct ttgcatcaaa
     2881 gctgatatct tcagcaagtg caaagtattt tccgaggctg tcatagtagg caaccgactc
     2941 aagatcatag gcatttgaaa taagatatgg cgaagtgctt gtgccatcac ccttgagcgg
     3001 cgtgcgtaca tcggatttaa tagcaaaatg taccggaatt cttatctcgc tgtccgaatt
     3061 tgacacatag taagccacaa tagaaatatc ggccatgtga tttactttaa gaaccagctt
     3121 atcctcgctc gaagaaacga cactgattct ttcctcagct tcggataccc agcccgttgt
     3181 gctgtcaaaa gaatatccct tgacttttgg aagattagaa agtgcgatct cagtcttgcc
     3241 gacagtcgat atattttcaa ccgaaggttt aatcgctgaa ccgttcggat tatccatatc
     3301 gacaatattc gtgctcttta tctcagttga ctcgccaaat gcgggaacat ttccgaaata
     3361 agtggaaata accgtgccgc tgactaacga atctatatta tcggagttta cagacttact
     3421 gtcctccaca aaaccgacca cgatgccggc atttttatta gttttctctg tttttatacg
     3481 ggcggcacat accgaatccg aaataacggc tgtcattccg ttgaagttgc caattccctt
     3541 tacaatgcca atcattccgc ctatggcctt ttcacccgat acggttccga gaatctttac
     3601 attcttgaac tggacattgt ttagtgctgc accgcttagc tttgcaactg ctcctgccgc
     3661 agcaacggca tcggcagaag aaacagtctc aacattcgca aacgacttaa tgttttcggc
     3721 aacaagtgca ctctctgcgc ccgttatatc tccgacaata cccgaagcaa cagacgttga
     3781 tataatcgtc gtcgcggagt caacatagcc ctcgtttata acaacagcac cgccggcgtt
     3841 ataaacggca agaattccgg ccgccacaga atttgcactc tctgcgcgaa cggttgtgtt
     3901 gcaaacattt gctcctttaa ttgaaagctt ggcatccata gctgccttgc cgacaattgc
     3961 cgaggcaacg cctgctttta ttgttgtatc acgaacctca acactgctga ttgaagagca
     4021 gaagcttgaa gcaataccgc ccgcaacaaa tgccgctgta tcggcaatcg ttgagatctc
     4081 tgaggaagaa acccgtgtat cagcaacgga aacattttct ctgcctgcgg caatgcccga
     4141 aatctcagac actcctttaa ttatcgatcc ttcaaccgat gcggatttaa ttgccgtctt
     4201 gccctcggca agaccgacta caccgccaac atattctgct ccgttaattt tagcattttt
     4261 aatctctgaa ttttccaaag cgctgtcaac ggaagcatag cctgcaagtg caccgacata
     4321 gtttgccttg gaattaaccg agcaattttc aactcggatt cccgacatgc cggatgttcc
     4381 ggaaacagag cctgctataa ctcccgaata aattccgtcg gaaacaacat tgctgttgac
     4441 aaccgtaatc tccgaaagct tggtgtttcc aaagacctgg cctgcaagga cagccgaata
     4501 aagaccctta gcatttacct gagccgaagc gattacaaga ttgcttacct cggcattttc
     4561 aagagcggca aaaaggcccg acttggatgt tccgctgatt ttcagattag aaatcgtgtg
     4621 tccgtttccg ttgaattttc cgctaaacgg aataagtgtt cctccaatcg gagcgaacgt
     4681 tgaaatctcg gacatatcaa tatcagactc gacaataaag ctcgacttgt taagctccgt
     4741 aatattttca ctgatatatg caagatcata tgctccggaa acaatatagt ttcccgcact
     4801 gtctttttca agtccgtttg aaattttgac ggttgtgtag agccccgtcg tcttgttcgt
     4861 cactgtaacg gcacagccct taacggttat aacacctgcg gcacgaccaa cattaagaat
     4921 aagctttccg ttttcaacgg ttactttcgc cgaaagaagg gatgtatcgg tcttaacaga
     4981 atagtcagaa gccgtcgaac cgctcattgc tttaacagac ggaagcgtaa cattgaactt
     5041 tccgccgttg ccggtaaaga aagctacctc accgtcgtca aatgaccact cgacaaattg
     5101 agatttatcg gcctttgcaa agtcggcagg agtctgcgta cccttgctca tatatatatt
     5161 tgaaatcctt gtatcgtcag cagaaatgtt tgcaataaat gagtattttc ctgcatcatt
     5221 cttgcccgaa acataacaat tttcaattat tacatcggtg gcactcaccg aaccgataac
     5281 gattgccgcg tttgtcgctc tcttggaagc ggcaatgttt gcaacagcac cgctgtttga
     5341 aattctcgtt ccgttaccga ttacagaacc taccagtgca ccgaaagatg caacatctcc
     5401 ggcaaaatta acctctgcct taacacggca attatcaagt tcactgtttc cggcagccgt
     5461 tccgaacatt acgccggaat ttacatcgga agcattaaac gctgcaccgt ttgctattgt
     5521 cacagaaaca ttttttgctt tcgcattaag tgcatttccg agtacagccg acgagagatc
     5581 ggtctttgaa acaaagttaa tatttgaaac cgttccgcaa acctcagtaa aaacagccgc
     5641 agaaacacta acggtatggc cgtttccgtc aagacttccc ttgaaagaaa aagcagaacc
     5701 gcttacggag atatccttac tgagctcagc atgaatgcct tgcgcatatt taagtaaatg
     5761 aaattcagca gtattttcaa caacaagcgg atcggaagct gttccgtcac caacagtatt
     5821 tgccggaacg acaagtatcg gtaaatcaaa gctctgcata atcttcagag agcctgttcc
     5881 gattgttgaa ggtaaagaaa tctcggtttt atagttaaac catgcaacct tatttgcagt
     5941 aacacccttt accgttaagc ccgaaactgc aattccggtt ccgccctgct taaagctgtc
     6001 accgattgca aagcttgcct ttccccatga tgcggaaagc gcagactttg agagagttac
     6061 agtttcgccg accttaacag tcttgaatct gagagtgtca atatcattga tatcagttcc
     6121 gtaattcgtt gcagatgtca ttcttccgct tacgtcgctt gaccaataac aatctgtaag
     6181 catctcggaa gatccgccaa cgccaacaag tccgttgccc gaagcactgc acttgagagc
     6241 tacgagaaca tagttgttca caaatttagc gccctttgac gccgagcctg caagtccgcc
     6301 gacaaaatcg gttgctttgc cgccgacgac attacccgaa gcaaagctgt tcgagataat
     6361 tcctgagttg gatcctgcta ttccgccgta aaccgnnnnn nnnnncgtta atatattcgc
     6421 cgacattgat tgcagagaca ccggtaattt taccgctgtt tttgcctgca attgctccga
     6481 ctgcgccgtt tgcggaaatt gtatgagatg cggcggaagc cccgctgaca ttgggctgca
     6541 ttcccgttac ttttacgccg gtaacggtac ctgtatttac agccgcaacc atacctgcgt
     6601 tcgcaacatt tttaagcgtg agaacagatc tgctgatctc acaatttgaa attgttccgt
     6661 cattttcggc aacaagaaca gcgcaattct ttacatcggt gcttacattg attacgcagt
     6721 tttcaacagt aagattttta attgttgatc tgctgttcac ataaccgaaa attgcaaaat
     6781 tttctccctt gccaaatgca atgttgagac ccttaattct gtgtccgttg ccgtcaagag
     6841 tgaagtaaac attctttgac gaggcgctga gatttttgct tgcggaaaca agcgaaccgg
     6901 aataaaccga gtttgatgta aaatcgcttg cattaaggct tgataaatca atatcacttg
     6961 tcagaacaaa gtttttattt gcacttgttg tgagattgac cttttcctgc attgcaacaa
     7021 aatcggttac atctgaaatc ctataaggat ccgatacggt gccgcttccc ttattgtatg
     7081 caacaagctc tccgccctcg gtagctatag ttgtggacga aattgctcca ccctcctgtg
     7141 aaactgtcgt ggaagaattt gctgcggagg cagagactgc agttgtagaa ccggaagtgc
     7201 caatctcatt ggcagccaga gacgatgcta ttccggttga ggacagcaac actgccgcac
     7261 tgagcaacgt taccgcaacc ctctctattc tccttttaat catagtaaaa cctccactgt
     7321 tttcactcac atcggctcca cgctaccgat gcagcggatc aacatatatt gttaaaataa
     7381 tatcagccgt tttttgctgt cttatttgcc ttgagcttct cttccttggc tttcgcacgc
     7441 tctctcgcaa ggcgttcctg ttctttcgcc tttgctcttg ccttttcgcg agctatacgc
     7501 tctttctcct tggctttttc ttttgcggcg cgttcctgct ccttgatttt ttcacgggca
     7561 atgcgttctt tttcctttgc cttttccttt gcgatacgct gtttctcagc ctcggcagct
     7621 ctcatcttag caatacgctc tttttcttta gcttttgcct tttctttcgc tgctttttcc
     7681 ctttctttcg ctctttcctt tgctaatctc tctttctctg cacgagctgc ttttgctgca
     7741 gcttctttct gcttaacctt ttctttagct atgcgatctt tttctgcttg agcagccttt
     7801 gctgcagcct ctttctgctt agctttttcc ttggcgagac gttctttttc ctttgccttt
     7861 tccttcgcta aacgttcttt ttctgcttga gcagcctttg ctgcagcctc tttctgctta
     7921 gctttttcct
//
LOCUS       DAWBSW010000090         7895 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1873_length_7895_cov_3.166454, whole genome shotgun sequence.
ACCESSION   DAWBSW010000090 DAWBSW010000000
VERSION     DAWBSW010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1873_length_7895_cov_3.166454"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    923..932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3653..3662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7398..7407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2269 a         1635 c         1914 g         2047 t
ORIGIN      
        1 ctcctacagt gctgagtatt cttgtgggaa caaacgacgg tcttttcgga tttttaaacg
       61 gattatcgct caaagaggtt gagggtactt acgaaaaaaa cctcacaaga atgattgagc
      121 tgacgagacg cgctctgccg catacaaaaa taattgtttg tgagcctttt tattttcctc
      181 tttcaaagga cgatttatcc tatcgttaca cgccgcatgt tgaatgtgaa gttgactatg
      241 gcagacccga cagagacgaa acgcaggaag ccatggatta taaatccgct gcaattcaac
      301 tggcgagagc ggcggcgagg agggtcgccg aaagctcggc ggaagttttt gttccccttt
      361 acgataagtt ctcggagaag attgcaggtt caaaaaccga atattttatg tgggacggaa
      421 ctcacccgtc aatcgcagga cacatgctta ttgccgagga atgggaaaaa gcagttgaaa
      481 aggcaaatat tttttaaaaa tatttatata accacttgta aaagcacggt tatatttggt
      541 aaaataaact tattaaaatg aaaagaggtc ttttttatgg cggatatggc aaaaaaagct
      601 ttttacagag catatcagaa aacctttatg gttgccgagt gcttctttga ttggtcggag
      661 cctgagcttc tcaagggtcc cggtgcagtt aaggatcttc cggcacttgt aaagtcaaag
      721 ggaatctcaa gcgttctcgt tgtaacggat aagggactta tgagtctcaa cctgcttgca
      781 ggcctttttg agcagcttga cgctcaggga atcaagtaca cggtttatga cggagttcag
      841 ccgaacccgt cgattgaaaa tattgaggaa gcacgcagac tctatctcaa gaacggctgc
      901 gaaggtatca tcgcattcgg cgnnnnnnnn nnagcgctat acataacaaa gaaaggaagg
      961 cgtatcatgg gaaaaatata tgcacgagtt tttcaagctt gttttaaggt tggcatggcc
     1021 gttttgcctt ggcgcatgcc ggaaactctg gagggtccgg actgcatcaa aaaactgccc
     1081 gccagaatca aaaaagacgg ttttcataaa gtacttgtag ttaccgacgc ggtcctgatg
     1141 aagctcggcg cgttgaacga tctgttcgca gctatggatg aggcaggcat tgcgtatacc
     1201 atctacgata aggctgaacc gaatccgacc gatatccatg tcaacaacgg cgtcgactta
     1261 tacaaggcct ccggctgcga atgcatgatc gcattcggcg gcggttctcc gatggactgc
     1321 gcaaaggctg cgggcgcaag ggttatgaac ccgaatcaga gtgtttctca gatgagaggc
     1381 gagctgaaga tcagacataa acttccgccg tttttcgctg ttccgacaac tgcgggtaca
     1441 ggctccgaga cgacccttgc cgctgtcgtt acaaatatgc agacccatga gaagtacgct
     1501 atcaatgacc cgaagcttcg cccgaagtat gcggttcttg accctgagct tacaacggga
     1561 cttccgcaga agattacttc aacaacaggt atggatgctc ttactcatgc cgttgaggct
     1621 tatatcggtc tgagcggaac aaaatcaacg aatgactatg ctgagaaggc aacaaagctc
     1681 attttcgaga acctcgaaac agcatacaac gacggcaaga atctccaggc tcgtgaaaat
     1741 atgctcgttg catcgttcta tgccggaatg gcattcacaa gagcttatgt aggctatgtt
     1801 catgcaatcg ctcacaatct cggcggtatc tacggcacac ctcacggact tgcaaacgcc
     1861 gttattcttc cgcacgttct tgagtattac ggtgacacgg cttacgagaa gcttgcaaag
     1921 cttgccgaaa tcgtcggtat cggaggatat tcgcaggcgg ataaggcaat gaactttatt
     1981 gacgctatca aggaaatgaa caggaacatg aacattcccg agcactttga ttttatcaag
     2041 gatgaggata tcccgacaat cgttgagcgt gcactcaaag agggcaatcc gctctatccg
     2101 gttccgaaga ttatggacgc aaaggactgt gaaaaggtaa tcaaggccgt aagaggcttc
     2161 taagagaata tctgtatcgg cggagcaatc tgccgatatt ttttaaattt ttcatgcaat
     2221 aagtgcattt tttgcaatta gatattgaca atccgctcac aatgtgataa aatatggtgc
     2281 aaattataaa gattacccct gcaatctgcg gagggaggga atacagtgag tcaggtaaaa
     2341 gttaaagaag gcgagtcttt ggacaacgct ctcagaagat ttaagagaca gacttcgaga
     2401 gacggtgtta tccaggaggt tcgtaaaaga gaacattatg agaagccctc tgtaaagcgc
     2461 aagaagaagt ctgaggctgc tcgtaagaga aagttcaaat aagaatcaaa acaaagaaag
     2521 actgttcgag aattcgagca gtcttttttc tcgcaaatat ttccggacag aaaaagaagc
     2581 cggaatgaac cgacttcttt ttccaataat tgaggggttt attgaggagg ggtatgtact
     2641 cacgagagtt gcggctctct cattgagtac agttagataa taacggctaa atgttaaccg
     2701 cgtatgaact ttctgttagc gtttggtata ttctttatga ataaagcacc gaaaataatc
     2761 ttaattattt gatcaaaaag ggttgaaatg cccgctgtga tgttgtaaaa tttaataagc
     2821 tataattacg caaagggagg tttttctatt gaaattagct gttcagcttt atactctcag
     2881 agaggattat tcaaacggtg aggaattcct tgaaattctc gaaaaagtaa aggaaatcgg
     2941 atttgacggc gttgagtttg caggctatgc aggacttgaa cccgaggtta tcaaggcgaa
     3001 gctcgatgag cttggactcg tcgcagtcgg atgccatatg cgtattgaaa actatgaggg
     3061 cgaaaagctc gaggagtcta taaagctcgg caaagctctc ggcatgaaat atatgggagt
     3121 aggcggtgcc gaccattcca ccaaggagga ggttgacaag cttgttgacg ttttctccaa
     3181 ggccaatgag cgcggcaagg acgagggcat caagttctac tatcacaatc acactgagga
     3241 atttgaaaac gaaattgacg gtaagctcat catggacaga attgccgaag gtgcatatct
     3301 cgaaattgat acctactgga gctactgcgg cggggcagat aattacaagt atataaccga
     3361 gcacaaggat gatattattc ttcttcacgt caaagacggt attgacagac atcctatggc
     3421 tctcggcgag ggtgacaacg accttctcag cgtattcaag gctgcaaagg acagcggcat
     3481 ggaatggctt gttcttgaaa acgatgatcc caagccgaac ggaattgacg atatcacaag
     3541 aagcatggca tatttcaaag caaatcttta atataaggag ctaaaaatta tgaaattagg
     3601 agtttttact tgccttttac aaaatcttcc tcttgaggcg gcacttaaat atnnnnnnnn
     3661 nntctctcgg aattgagatg atcgagcttg gctgcggcgg cttcccggga aacgcacact
     3721 gcgatcccga gacacttctc aacgatgagg aaaagttcaa cgagtttgtt gcaacgatta
     3781 aaaagtatga tatggaaatc agtgcgctga gctgtcacgg caatcccgtt catcccgata
     3841 aggaaaaggc tgccgcattc gataagacga ttcgcgatac gattcttctt gccgaaaagc
     3901 tcggacttca tcagatcaac accttctcgg gctgtcccgg cgactgcccc gaatcgaagc
     3961 atccgaactg ggtggtatgc ccgtggccgg atgatttcag cgaggttctt aaatggcagt
     4021 gggaggaggt tctcgttcca tattggaagg atcttgttgc gtttgcaaag gctcacggag
     4081 ttaacaaaat cgctcttgaa cttcaccccg gcttctgcgt ttacaacacc gagagtcttc
     4141 tcaagcttcg tgaagctgta ggccctgaga taggtgcaaa ctttgacccg agccacctca
     4201 tatggcaggg tatggatccc gtcgcatgta tccgtaagct cggcgatgca attttccatt
     4261 tccatgcaaa agatacgaag atcgacaagt acaacacaat ggtcaacggc gtgcttgata
     4321 caaagccgta ttcggacgaa atcaaccgtt cgtggatttt ccgcagcgta ggatacggta
     4381 acgacgagct ttattggaag gatattatat caaatcttcg tcttgtcggc tatgactatg
     4441 caatcagcat tgagcatgag gacagcctta tgagtcagaa cgaaggcttg acgaaggcgg
     4501 ttcagacgct taaaaatgca cttatcacag aatccacaac ggatatgtgg tgggcataac
     4561 cgtttttgaa gtgggtcaaa taaacaaatt ctcgccaccg caagcggttt tcctcctctt
     4621 gtttcaaggg gaggtttggc aattttgcaa agacttttta aatattagga gcaaaacaaa
     4681 atggtaaacg gaaaaatcgg cggcgcagtc atcggctgcg gcggaatggg cagttggcac
     4741 gccgaaaaga tgtcaaaata tatgaatgac cgtatggagc ttgtcggaat ctacgacatt
     4801 aacccggcaa aaaacaagga agccgaagaa aagggctggc atgctttctc ctctcaggag
     4861 gagcttcttg acgatgacag aattgatttt gtagttgtcg caaccccgaa tgacttccac
     4921 catgatattg taattgccgc acttgaagca ggcaaaaatg tcatgagcga aaagcccgtt
     4981 acaatgtcga gcgaatcact tgacgatatg ataaaggcga gcgaaagaac aggaaaattt
     5041 ttcaccgttc atcagaacag aagatatgat cccgattatc tcaccgtcaa gcgtctttat
     5101 gacgagaaca cactcggcag gattttcagg atcgaatccc gtgtaagcgg ctcgagagga
     5161 attcccggag actggcgtca gagaaagtct cagggcggcg gaatgattct cgactggggt
     5221 attcatattc ttgatcaggc actgatgatg tttgacgaca aggagcttga ctcagtttac
     5281 tgtcaggtga ctcacattac aaatgacgag gttgatgacg gatttatcgc aacgctcaac
     5341 tacaaggatg attcggctta tgttatctcg gtaggcacga ataactttat tgattatccc
     5401 cgctggcatg ttcagggcat aaacggctct gcaatctgcg aatgggatct cagcggcaag
     5461 gtcgttatgg tttccgattg ggagaacaag gacgccgttc ccgtcagaac cggtgtaggc
     5521 cttacaaaga caatggcacc gagaaccgac gattcaataa agactttccc gcttccttat
     5581 gttgaggata agggcttcag cggcgagcac acgctctaca acaacatctg tgatgttatc
     5641 gagggcaagg ctgaacctat cgtaacccat gctcagcaga ggagactcat caaggtcgtt
     5701 gaagcaatgt ttgagtcggc agaaaagaac tgcgttgtaa aattctgatt aaactgatat
     5761 agagagctgt tgcttgtaac tctgtgtcac ctgatacttg taaggcttta ggtgatacag
     5821 agtactaagg gcgacggctc ttagtttttt ctgcttaaac ggcttcgttg tatgtaatta
     5881 aaaagaatat ccaaaaattc agacacactc ggcatgaatt caaggtgata tcctgccatt
     5941 ttttcgaggt cgccggggtt catgcgccac gcacgctgaa ccacggttcg cagatttttc
     6001 tctatcgccg atggggttga acctaaacgt tttgcaactt ccgtgtaaat ttcgcgggtg
     6061 attgaattca gtcggttctc atcctccagg gcaagcgtaa gagcaagaac cgttgctttg
     6121 tagcccttat atgtagtgca aataccgagc cttctgagaa ttatttgaat ttccatattt
     6181 ttacctccgt tttattaagt cggcatcgca caaattgcaa tatgtacata ggctgtgcgg
     6241 tgttgcatta aattatattt ttatttgatc attttttcga aatgtcggct tttatatatc
     6301 tttttttaca aaaaaacctt taaactgcca aaaatcgaca aaaacaccga ttatatcgca
     6361 ttcggttatc gggggtatag cttgccgccg aaaaattact gcgatttaat cagtggttac
     6421 tatatttttg tgtaaaacaa gctgattttc tttcctcttg acaaacattt cctctttgat
     6481 atacttaata tattcaaggc attttatttg ctattgtctt aattttcgga ggacgtattt
     6541 atggaaagaa aaaaatttaa aagtattatt gctgcggtca cggcaatact catttttatt
     6601 gttccgatgc tttctgttgt cgtatcggcg aagaatgaca cggtaccgcg tatctggatt
     6661 cacggattca tgaattcaaa ggtttatgag gatgtttccg atccgaactc gccacttgca
     6721 tggccgccca cgcaggagga aatcacaaca gcagttgaag aaagcgtgcc tgcctttgcc
     6781 aagctggctg taacaaagga ttgggacacc ttcggcaaag agatgggtga aataacgggc
     6841 aagctttttt cgagggcaat gaacaaccct gacggaacgg tcagggatgg ctccggaatt
     6901 cgctttgaat accctgcaaa gagcaaggtc aaggccgaca aggatattta ttttaagtat
     6961 gactggcgag aagatccgat caagattgcg tcagagctca acgattttat aaactatgtg
     7021 cttgatgcgt cgggctgcga tcaggttgcc ctaacatgcc acagcttcgg cggagttgtt
     7081 gcgacaacat atttttcaat ctacggctgc gataaggtta agacggtcgt gtttaactca
     7141 acggctattt acggcgagac ctacacggga gaattgatga ccggcagact ggctttcggc
     7201 ggcgacgctt tgcagaatta tcttttttat gctttccagg gcaacgagta tgaaaacctt
     7261 atcaatgcgg caattaaagc ggttcacgat acgggtctga tggatattct cacaaaaggg
     7321 ttgaacggat taagcgcaaa gatcgaaaaa gaggtttgtt cgcagtcgat cgttccgctt
     7381 ttcggaggct ggcttgcnnn nnnnnnnttg tttttaacac tatttacggc aactcggaaa
     7441 ccgattattc cgtattgaga gcgaaaattg aaaattataa caaccttgtc cgcagtaaaa
     7501 agaccgagac gctgaaagag gtcaacgaga ggtgtaatgt ttatgtaatt acccgttacg
     7561 gctactcgtc acttccgatg actccgtccg caagcaacct tggcgacgga ataattgacg
     7621 ttaaatatgc ttcgttcgga gcaacggcaa gcgaatacgg caagactctt aaaaatgttg
     7681 cgtcagatgc aaaattcatt tcacccgacg aaaccgttga cgcttcgacc tgccttttcc
     7741 ccgagcagac atggttcgtc aaaaacatga agcatggcag cgatgaagag tctttgaaca
     7801 atatgatgcg cagatttatg agatattacg gtcaggcaac cgttgaaacc tttgaggaat
     7861 atccgagatt tacggtcttt gacggcgaga agatt
//
LOCUS       DAWBSW010000091         7875 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1878_length_7875_cov_2.498210, whole genome shotgun sequence.
ACCESSION   DAWBSW010000091 DAWBSW010000000
VERSION     DAWBSW010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1878_length_7875_cov_2.498210"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1582..1591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4012..4021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4547..4561
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2158 a         1666 c         2123 g         1893 t
ORIGIN      
        1 ccgtagctcg aagtcacggc cttgagattg caagcggaga ttatattatg ttcgttgacg
       61 gtgatgatta tctgcattat caggcagttg agctgttcgt cagagagatt gaaaaatcgg
      121 actgtgacgt tgtctgtgca catgaagttt atacgtcgaa taccgatgaa aaaatgcagc
      181 ccgtcagtga atataaaaca cgcgaaataa ctgttgaaga attgtttgac aacagaaaaa
      241 acaggagcat gggtaaatcc gtttggggaa aaatcttcaa gaccgagatt gcccgtcgga
      301 gccgtttccc ggttggaatt tcaaacggag aggacgctta ttacatcata actctgctcg
      361 ataagggcat agcggtgagg tgtatagacg ccgagctgta ttattatctc aacagacaga
      421 attcaacggt tacatcaagg tttacaatga agcgtttcac gatgacatat tctttcgata
      481 tgctttgtga aaggctgaaa aattcatcaa attcattctt gaaaaaatac tgcttgcaat
      541 acctgtttca gtcgattttc tataacagaa cgcaggcgat tggcacgtca tgcgaaaaag
      601 aggtcatttc cgagtcgaaa agaataggga aaaaatggct aaaggatttt caaaggaata
      661 aagatatttc gccgattata aaaatatcgt ttacagtgtt cttttattca cggcatgtat
      721 acgagcttgc aagggcgata caggatccga caatgattga tttttataaa agcaggaaaa
      781 aggagaatgt ccgtaatgaa gcttgaaatg agaaacaaag acgattgcac ctttgatatg
      841 atatcactcg gtgagataat gctgagattc gatcccggtg attcgagaat aagaaccgcg
      901 cgttcattcc gagtatggga gggcggcggt gaatacaacg tcgcaagagg acttcgcaaa
      961 tgctttggac tgaaagaggc ggcggttacg gcgaatctgt tcgcgcggac ggcggcggtc
     1021 acggcgctcg ccgataatga gatcggcagg cttgttgagg atttcatgct tcaaggcgga
     1081 gtggatacct cgctgataaa gtgggtgaaa tacgacggca tcggaagaac ggtcagaaac
     1141 ggactgaatt ttaccgagcg aggctacgga atccgtggag cactcggagt ttccgacagg
     1201 ggaaacaccg cctcctctca gctcaaagag gcgatatcga ctgggagtac attttcggca
     1261 ctctcggtgt tagatggttt cacacgggcg ggatttttgc cgcactctcc gacacaacgg
     1321 ctaaggttgt tatcgaagcc gttaaaacag cgaaaaaata cggaacaaca gtttcctatg
     1381 acctcaatta ccgaccgtct ctttggagcg atatcggcgg caaggagaag gctcaagagg
     1441 tcaacaggga gatcgcaaaa tatatcgacg tcatgatcgg caacgaggag gatttcaccg
     1501 cttgtctcgg acttgaggtt gagggcaacg acgtgaattt aaaggagctg aacatcgacg
     1561 gctacaaaaa aatgatggca annnnnnnnn ngtcaaaacc gctactgtca acgattggag
     1621 cgcaatatgc tgggctgacg gcaggattta taacggactt gattttaagg gccttgaaat
     1681 ttatgaccgt gtcggcggcg gcgacagctt tgcttcggga cttatttacg gtttaatgaa
     1741 aaccggcgat ccgcagacgg cggtcaatta cggtgtggct cacggtgcgc ttgccatgac
     1801 gaccccggga gacacttcaa tggcaacgaa agccgaggtt gaaagaataa tcggcggtgc
     1861 tggtgcaaga gtacaaagat aagcgaggat gatttatgct atataagaaa aacagcgaaa
     1921 aaaagctttc aaaggagctt tttaaaaatc cgacatgtga attcagagga acaccgttct
     1981 gggcatggaa cagttggctt gaaaaggacg agcttgaaag acagaccgga attttcaagg
     2041 aaatgggctt cggcggattt catatgcacg tcagaacggg acttaaaaat aaatacctca
     2101 gcgatgaata tatgcagctc ataagagact gcgttgaaaa ggctaaggat gaaaaaatgc
     2161 ttgcctggct ttacgatgag gacagatggc cgtcgggagc ggcaggcgga tatgtaacgg
     2221 aggatgtacg gtacagagcg agatatcttc tttttactcc gttcaagacc gcagaggcta
     2281 agaaatccgt tgaggtcagc gcaggcagaa ccaataacgg aaaattgctt gcctgttatg
     2341 atgttgtgct tgacagggac ggatacttgt cctcttacaa gcgaatcggc gaggacgatg
     2401 atgccgaggg cacaaaatgg tatgcgttta tggagattat aggcgagtcc gattggttca
     2461 acggcaaaac ctatgccgat acgctctcaa aggacgcagt cgatcggttt gttgaggtca
     2521 ctcacgaaaa atacaggaaa tgcacaggcg acgagtttga caagaccgtt ccggcaattt
     2581 ttactgacga gccgcagttc actcacaagg cggttatgaa caattccttt gataaaatgg
     2641 acatgataat gccctggacc gacaaggttc ccgagcttta cgaaaaggct tacggcgcag
     2701 atatttatgc gacgctcccc gagcttttct gggatctgcc ggagagcaag ccgtccgtgc
     2761 acagatacag atatcacgat tttatttcag agcttttctc aagctccttt gccgacaata
     2821 tcggctcatg gtgtcgcaaa aacggaattg ctttaacggg acatatgatg gaggaaccga
     2881 cgcttcgcag tcagtgcgcc gcactcggag aggctatgcg ttcctacagg ggctttgata
     2941 tccccggcat tgatatgctg tgcaactggc acgagttcac aacggcaaag caggcacagt
     3001 cggcggttca ccagttcggc tatgagggta tgctctccga gctttacgga gtgacggcct
     3061 gggactgcga tttcagaacc tataagcatc agggcgattg gcaggcggcg ctcggcgtaa
     3121 cggtcagagt tccgcatctt tcatggtatg caatgggcgg cgaggcgaag cgtgactatc
     3181 ccgcttcaat ctcgtatcag tcgccgtggt ataaaaaatt ctcggttgtc gaggatcact
     3241 ttgcaagact caatacggct ctgacaaggg gcaagcctgt tgtcaaggtt gccgtcattc
     3301 acccgatcga gagcttctgg cttcactggg gaccgaacga caagacagcc gttctgcgtg
     3361 agagccttga cgaaagattt gataacgtta caaactggct gcttgagggc agtatagatt
     3421 ttgattttat ttcggagtcg cttcttccgt ccctttgcga gaagggctca aatccgctca
     3481 gggtcggaaa gatggaatac gatgcagtca tcgttcccgg ctgcgaaaca ctccgctcaa
     3541 ctactgttga caggctttcc gagttcggca aaaacggagg caggctcata ttcatgggcg
     3601 acgcgccgta tctttgcgac gccgttgaat ccgacagggg caggaagctt tttgaaagaa
     3661 gcataagaat tgatttttca agagcttcgc ttctcaatac gcttgaaagc gacagaacag
     3721 ttactttgag atatgccaac ggcagactga cggataacct tatctatcag cttcgccgtg
     3781 acaacgactg cgaatggctc tttatctgcc acgattcgga accgaaaatc aaggatattg
     3841 attacggcag tgacataaaa attacggttg acggcgagtt tgtgccggag ctttacgata
     3901 cgcaaagcgg tgagataact ccgctcggcg cttcgtatat taacggaaaa accgttattt
     3961 cacataagct ttacagctac gattcgcttc ttataaaact gaccgacggc annnnnnnnn
     4021 nagggcaagt cggttgtcgg cttggtggac tatgagctcg acggaaagaa cgtgcttctg
     4081 ctcgatatgg caaaatttaa gctttcgggg gagaccgatt tttctcccga ggaggaaatt
     4141 ctccgtcttg ataatatctg ccgtgagcgt ctcggacttc cgcttcgagg cggttcgata
     4201 gttcagccgt gggtttcggg cgatattccg ccggtggaca ggcttacgct gcgtttcact
     4261 ttcgacagcg agattgatta cagcggtgcc gagcttgcat ttgaagagcc ggacagactt
     4321 gaaaaaatcg tattcaacgg cagtgaggtt gcaaaagagc tttgcggcag ctatgttgat
     4381 atttcgattt tcagagtaaa gctttccgac attatcaagg gtaaaaatac acttgaattg
     4441 acatatgcat acggcgaaaa gaccgacatt gagagcgtgt ttattctcgg cgatttcggc
     4501 gttaaggtta cgggaaccga aaaaacagtt atcgctttgc ccgaaannnn nnnnnnnnnn
     4561 ncataacaag acagggcttc ccgttctacg gcgacaatat tacatacaaa ttcaaggcag
     4621 ttgcagaaaa cggaaaattt gatatctgtg cttcatggta caggggtgca atgattttcg
     4681 tcaaggttga cggcgaggaa aagggtaata taatctatcc gccgtacatt ctcggcgttg
     4741 acggactttc cgacggtgag catgatgttg aaatgacgct ttacactcac cgctacaaca
     4801 ccttcggccc acttcacctt gtcaatgagg ccgagccctg gcacggaccc gacgcatgga
     4861 gaagcaagaa gtgcaactgg agcaatgaat atgttctcag acgggtggga atactttctg
     4921 cgccgaaaat caataattga cggaggatac tatggataaa gaaaatattc ttatgagaat
     4981 tcaggactgc ggcattgtcg ccgttgtcag agcagaaaat gccgacaagg cggaaagaat
     5041 cgttgatgcc tgcatggagg gcggagttgc ggcaatcgag ctgaccttca gcgttcctca
     5101 tgcggacaga gttatcgaaa ctctctcata taaatacgac cctaaggaca taatactcgg
     5161 tgcaggtact gtcatggacg ccgcaacggc gagaatcgca atgctcagcg gtgcgcagta
     5221 tattgtaagc ccgtatcttg atcttgaaac gctcaggatg tgcaaccgtt acagagtgcc
     5281 gatgatgccc ggcgttatgt ccgttcgtga ggcggtaacg gcaatggaga acggcgcgga
     5341 tattctcaag gttttcccgg gcgatctctt cggacctaag ataattaagg atattctcgg
     5401 tcctattccg tatgcaaaga tgatgcccac gggcggagtc gatgccgata atgtaggcga
     5461 atggataaag gccggtgctg ttgccgtagg agcgggttca tcgctcacgg caggcgcaaa
     5521 aacaggcgat tataagctta tcacagagac ggcaaagaaa tttaaagcaa acatcaaggc
     5581 ggcaagagcc tgacataatt taacagaatg aggtaaatct atgtttgtag acatagcaaa
     5641 aattaaaata acggcaggcg acggcggcaa cggagcggtt gcgttcagaa gagagaaata
     5701 tgttgccgca ggcggtcccg acggcggaga cggcggcagg ggcggaaacg ttgtgttcaa
     5761 ggtcaacgac aatctttcga cgcttgcaga tttcagatac aaaagaaaat acaaagcccc
     5821 gaacggtgct ccgggtcagt caggccacaa gagcggaaaa aagggcgagg acttaatcat
     5881 tgaagtgccg agaggtacta taattcgtga acagactaca ggcaaggtca tggcagatat
     5941 gtccgacgat gacgagttca ttgccgcaaa gggcggcaga ggaggctggg gaaattccca
     6001 tttcaagacc ccgacccgtc agacgccgag attcgcaaag cccggcacgc ccggcgagag
     6061 ctgggacgtt tcgcttgaat tgaagctcct tgccgatgtc ggacttttgg gattcccgaa
     6121 tgtcggcaaa tcaacgctta tttccgttgt cagcgaggct aagcccatta ttgcggatta
     6181 tcatttcacg acgctcactc ccgtgctcgg cgttgtttcg atgggcgagg gcaattcgtt
     6241 cgttatggcg gatatcccgg gactcattga gggcgcaagc gacggcgtag gtctcggcca
     6301 tgaatttctc aggcatgttg agcgctgccg tctgctcgtt catattattg atgccgcagg
     6361 cagcgagggc agggatccga ttgaggattt caacaagata aacgaggagc ttgttaagtt
     6421 caatcccgat atggcggatt tgccgcagat agttgtcggc tttttgcggc gcagataggc
     6481 gaaaacagcg tcgaaaagct cgaaaaatat ttcaccgagc gcggatatca atattttacc
     6541 atgtgcgctc cgattgcaga gggtacgcag gagattatca atgcagttgc ggcgaagctt
     6601 gcgacgcttc ctccgataaa gagatacgaa aaagaggaga ttcctgccga gttctttgag
     6661 aaaaacgcag acggaaagtt tacgatttcc gttcaggacg gaatatacag cgttgagggc
     6721 gagtggcttt tgagaatact ccagcgctgt gatctcgacg attacgagtc tttgcagtat
     6781 ttccagcgtg tgcttcattc cagcggaata atcgacgcgc ttgtagaaaa aggaattcag
     6841 gagggtgaca cggtcgagat atacgatctt gagttcgact ttgtgccgta aaatatatgg
     6901 aacgtttttt gaaagataaa gaagtaaagc ccaacgaatt aagccctttg acgcttgctt
     6961 ttatcggtga tacggttttt gacctgcttg ttcgcgagga gcttatctgc gatgcaaaca
     7021 gacccgcaaa cgacctgcat cacttggcgg ttgagcgggt cagagcgacg gcacaggcac
     7081 agggcgttga aaaaatcatg cctgttcttt ccgaggagga aaccgccgtt tttaagcgag
     7141 gaagaaatgc aaaatcggga catcagccga agaatgtcag ccgttcggat tatcatatgg
     7201 caacggcgat ggaaactctt ttcggctacc tctatcttat gaacaggacg gacaggattc
     7261 ttgaattata caggttgatt tacggttaag gaggataact ttgcaaaaga aaaaatcact
     7321 tattcaggat ttaaaaaaat acagtctgaa aaagtttgcc gttgataacg tatattttgt
     7381 catcggctgc cttctttatt ccctcggagt caatattttt gcaattccga ataaaattgc
     7441 acagagcggc attaccggcg ttgcaattat cattaactat ctgtttccgc agtgcccggt
     7501 aggacttact ggatttttgc ttaacgtgcc gctttttatc ctcgcatgga ttttcatagg
     7561 caagtttttt acgttcaaaa cgctgtgggt atcgggagtt ctttccttaa taatcgacgg
     7621 cgtgaagctt gccatgaaca ggggcttgat aggcgcatat gagggcgaca agctgcttgc
     7681 ttcgattttc tgcggcgcaa tgtgcggagc gggaattgca cttattatcg taagaggcgc
     7741 aacttcgggc ggtacggatg tactcggcag gctgctaaaa aagatttggc cgcatatgtc
     7801 aatcggcaga atgataatgc tttgtgatgc ggcggttgtt atagcggcgg ctgtcgtgtt
     7861 caggagtata gacag
//
LOCUS       DAWBSW010000092         7725 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1915_length_7725_cov_2.718905, whole genome shotgun sequence.
ACCESSION   DAWBSW010000092 DAWBSW010000000
VERSION     DAWBSW010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1915_length_7725_cov_2.718905"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2141 a         1871 c         1557 g         2156 t
ORIGIN      
        1 acactatcga caaaaagctt gccgacgtta actgcgacgg cacagttaat tcagccgatg
       61 cgctcatcgt tctcagagtt tgcgtgggca cttatgaggg gccgacagag gttgacctca
      121 agcccgaggt cattgacccc ataatgaaaa ccagaaagtt caccctcggc acagttgttg
      181 acacagtgga cgaaaaagga aattcagtca gcattccttc caccgtcatg gtaagcggca
      241 acaacatttg cacttccatg aaatacagcg gcatgaatat cagacttctt gttcttaacg
      301 gcaaagtata catgataatg ccggacttga aggtctatgt ggaaatgaga aaggaagatc
      361 tcggcggtct tgatttcggc aatatgaatt ttgatgataa tcagacctat gccggttcat
      421 tctactcgac atataacgga aagacctaca ccgtcgattc ctataaatca tcggatggtt
      481 cggttgccga ttactatttc cttgacggaa aatgggtgat gaccggcgaa cattccgcaa
      541 agcctgagga agcaatgaaa attaccgagt tcaaagcagg cgtcaatgaa tcatattttt
      601 cacttaaagg attcaaaaag attgacatta ataaataaag gagataattg aaaatgaaca
      661 agtttattaa aacaggtatt tcaattgctg ccgcagttgc cattaccgct tcggcttctg
      721 caatggcttt ggcagcgaat tacatgggtg acgtcaacga tgacggtaaa gtaaattccg
      781 cagatgcgct ggcaatactc agatacacag tgggtattga cggcacagag atcaatctta
      841 aaaaagccga tgtcaattcc gacggaagca tcaattcttc cgacgctctt aaggttctca
      901 gaatgagcgt aagtctcgag gatcttattg aaattaaaga agaaaacaat gaggaggaga
      961 aaactcctgt cgactttgac aaggctgaag ctgttgaata ttacaacaat gcgctcaaga
     1021 aagcttatgc aagcgaaaat gtaacaatag acaagaaaac agatgttaag gtcaagatag
     1081 ataatctttc tgctttcaag gatctcgcca attctctgat agacaagtat gcggttccga
     1141 ccaaagctac agaaacattt aaatctgaac cggaaaaggc cgaaaagttc cttgttccga
     1201 cagcgcttga agcagacgga gcaaaagagg caaaggttga ggcaacggag aacggctata
     1261 aagtaactat tactcttgtc agcgaaacag ttgactacaa aacagccccc aagtataaca
     1321 cgcaggcttc tcttccgctt acaggcattg cagagcttgc cgctcagaac aatgtaacgg
     1381 ttaaatcctc gtcatttaac tattccggta cggtcctcac ggctgaaatt gataaggacg
     1441 gaaatatcct ttcgctcaat cacactatgc cgcttaagct ttctgctaag gtaagatacg
     1501 gtcttatgaa tatcgaaggc tcaggctcgg gtaagtacac tcttgacgca acgttcactt
     1561 attgattcat aaaagcatta tacacataaa aagacggccg atttaatcgg tcgcctttct
     1621 ttttttgaaa ttcattataa atttgttaag gtacttcaac gggaagttaa aaatatcgag
     1681 tgagaccgca aacgtcaaaa tcacaccgat aacaagaagt aacaaaattc cttccttggt
     1741 tcttacaagt gttttcatgt ccaaaagttt gttcataagg acgaggaacg aatagtgcca
     1801 aaaataaaca tttaccgtat ttctgcccat gtaagaaaca atcggtaatt ttttttgcgg
     1861 aactacaaca agaattgccg cacccataat caaagtaagt atatgtgcca gcaacctgta
     1921 aaggcaaccc caaggcaaga tatcttttct aaaaggattt cttccgctga aaaggtgttg
     1981 caggattttt acatcatcga atttccaaaa gcataccacg caccagcata tcagcaaaat
     2041 cagtgccggg gcaaaaagat acttcttgta ttttttaatt ttctcaatta agatattctg
     2101 atcaatcatt gttccgagcc aaaaatacgg gaagaatacc aacagtcttg atatacaaag
     2161 gaaatcgccg atatttttaa agtatcccgt aaacaatgat atcgcaaatg aaacgatcag
     2221 gacaaccgtt ttgtttgctt ttttcagtag ccacgcaatt gcagtgaacc atgcaagcac
     2281 aaacatgaac caaggcactc cgccctcata aaaaagtccg aatttcgcct tgccgtttat
     2341 cgccagatat gagatataca gcaatccctt taacaaaaaa gccgacgaaa gatagaacaa
     2401 tatttttcta aggcaatcct tttctttata gaacaatccg aaaataaaaa taaacatcgg
     2461 catatgaact gtgtaaagaa aaagatttat cgccatgaca tcttctctgc cgtatgatgc
     2521 aatatacccg acaacatgac cgacaacgac aaaaagaatc ataagaaatt taacggtatc
     2581 ccaataccaa attctctctt tctttattaa attgttattt ttcattttat aatcctttat
     2641 ttttgagcag ttcggctcat ttgatatcgg aatagaattc ctcaatatac ttgtcaacat
     2701 cggctctcgt tcccttatac ggtccgggaa tttccatctt gttggaaacc gttgccgatg
     2761 cataaagaag cgaatccttc atggaatgat gaagtctttc tgtcatgtaa gccgcaaacg
     2821 tcgtatcacc gcggcctgta cgtccgctga gatttcttgc acgaatcgga catgagcaga
     2881 attccttacc gtcatatgca ataacctctg tgttatgagt gataacaacc tcttttgcgc
     2941 cccatgtatg gaggagcttt gccgcctcgt atctgtcggt gagacctgta agaatctccg
     3001 cttcggcggc gtctgtcttg agatactcga tatacggaag aacctctttt ttctccttcc
     3061 aatcctcgaa aaacattgta tgatcctcaa gattaacatg gcggagaaga gcctgaacgt
     3121 caacggcaac cttgcccctc ttggaagctt caataatcat ctcgtttgtg aaatcgccgt
     3181 agagcaaacc tgcaaagtga taaatatccg cttcaacatc ctcggggata tcatcaatcg
     3241 tgaacggagt acctacactt atgcacatac actttctgcg ttccttgtcg gccgtgaaat
     3301 actgattatc catagtgcat gacgactcgg acatgatgca atatatatcc tctttcggaa
     3361 tcgtaaaata tgtaagtctc tcgctgtcgt cgggatttat ctttgtcaaa gcggcaacct
     3421 tgtgcccgag agcatatgcg gacgccgagg aataaataac cgctccgccg attgcaagag
     3481 tttcatttcc gaggtaatcg gtatcatgat ccattgtgat atgaccgata ataagtgatt
     3541 cgtaatgctt cattattctg ctcctttgtt tttatcttcg taattcagta tttcttttgc
     3601 tatatatatc ggtctttcct tgacctgaag ataggttctt gcaaggtatt cgccgattat
     3661 gccgaggaaa agagaattga gtccgccgag gaaaagcaga atcgttataa ttgcgatcgt
     3721 acctgtgatc tcgccgacta cggctccgac gatcaaagcg attatgagat aaagcagaga
     3781 aatccccata agacctacac ctgcatatga tgccgccttg agcggaattg tagaatagcc
     3841 gacaatgcct tcgattgcat atctaaaaag cttcatgaac gaccattttg acgtacctgc
     3901 cgccctctcc tgcacctcat agggaatata ctttgtgtta tagccgacaa aggagaaaat
     3961 accctttgag aagcggctgt attccgacat atcgagaacg gagtcaagca tctgacggcg
     4021 cataagtctg aaatcgcttg ctccgggata aagctcaaca tccgagagct tgttgattat
     4081 ctcgtagaac ttatccttga agaaaatcaa aagcttactc tccttacgct ctccctgata
     4141 agcggcgaca cagtcaatat ccgcattttc atcaagaatt tgcttcatct caagcacaac
     4201 ctcggggcgc tgctgaagat cggcgtcaat aacgcaggta tagtctccgc tcgactgctt
     4261 caagccggca taaatagccg cttccttgcc gaagttacgc gagaaattga caaccttgac
     4321 attatctgcc ttttcgtata tagctctgag tatctgcggt gttttgtcac ggcttccgtc
     4381 gtttacaaac accagttcat aatcctcaac cttgtcctta aaaacacgtg taacctctga
     4441 ataaaattca attacacttt cttcctcatt aaagcaagga acaacaagag ttaatttcat
     4501 ttaattcacc caattctttt aaaaatctgt ttaaagcgtc ctgccatgtc ggcagccgtt
     4561 tgattcctgc gtcatcaaga cattttttgc tcattcttga gttgtgcggt ctttcggctt
     4621 tcaccggata ttcgcttgaa gcaacaggat tgatctttgc ctttcttcct ccgagcttca
     4681 ttatttcagc cgcaaactcc gcccatgagc agtagttttc gtttgtgccg tgatatattc
     4741 cgtacttatc cgtcacaatg aggtcacaaa taagccttgc gagatcggga gtgtaggtcg
     4801 gtgaaccgat ttggtcacaa accacattta actcgtcctt ttcacttccc agtctgagca
     4861 tcgttttaac aaaattgttt ccgtttatgc cgaacaccca tgaggttctc acaataaaaa
     4921 atcggtcgag atgcctcata accgcctctt cgccgtcaag cttagttctg ccataaacat
     4981 tcttcgggtc ggtcgggtcg gtcgtctcaa acggtttttc gccggctccg ccgtaaacgt
     5041 aatccgtgct gacatagagc attcttgcac cgagcctttc gcaggcaatg gctatgttct
     5101 ctgttccgtc aacattaact ttacggcagt tctcctcatc atcctccgcc ctgtccactg
     5161 ccgtatatgc ggcgcaatga acaacggcat cgggagcgta gccacagata taatcaaccg
     5221 tttcttcacg gtttgttata tcaaaatcct ccctgtcaac tcccttgcac tcaattgagc
     5281 gtgagttcaa ctccttgata acatcaaagc cgagctgacc gttatatcct gttacaagaa
     5341 ttttcatgtt ctgtcctcaa aaatcaaagc ttacgtcgct gtccttgaga agcggagcgg
     5401 aaatatcctt ctttgaaaga ataggattat caattcccca ttcaacgcct atctcgggat
     5461 cgttgaactt aacgcttctg tcgtcctccg gacaatagaa ttcatcaacc ttgtacataa
     5521 cctcaacgtc gtccgtcagc gtaacaaagc cgtgcagaca gcccttggga ataaaaaagg
     5581 atttcttgtt ctcgcccgaa aggataaccg acgtccactt catgtaagtc ggcgagcctt
     5641 ttctgatatc aactgcaacg tccataattt cgccgtgcac gcaggtaagc agcttactct
     5701 gagcggtcgg gttgacctga cagtgcaagc ctctgagcgt acctttctcg gcagaaaacg
     5761 acctgttatc ctgaataaaa tcaacatcta taccaacctc ggcgagctta cgtttggaat
     5821 aagactccat aaaccagcct ctgttatcgc cgaaaacatc gggctcgatt accattgcac
     5881 ccgttacttc tgtttcatat gctttcaaat atgtcagtcc ttttctttta gcaaatttgt
     5941 gcgatatcgc ctttacatct tttccgttga acacggcggc tcctgctccg tgccccagcg
     6001 aggacgttcg ccctgcttat cggaatacat gatcttgccc tcggcaactc tcataagatg
     6061 ctgaccgtaa ggcgatttgc catactgctg cgcggattcg ataagagttt tctttgaaat
     6121 ccagcgcatg ttatatgcga tctcctcggg tgcggaaatt tgaataccct ggagcttttc
     6181 tatcgtcctg atgaaattcg ccgcttccat aaggttatcg accgttcctg tgtcaagcca
     6241 tgcgaagcct ctgcccatga gcttgatatc aagatcgccg ttgtcaagat aaatctggtt
     6301 gatatccgtt atctcgagct ctcctctttc agacggttta aggctcttgg catactcaac
     6361 aaccctgttg tcgtagaaat agagtcccgt aattgcataa ttcgactttg gctccttcgg
     6421 cttctcgaca attctctttg gcttgccctt tctgtcaaat tcaacaacac cgaaggcctc
     6481 cggattgtca acatagtaac cgaaaacagt tgctctgtcc ttgttgagag cggcctgcct
     6541 gagcatatga ccgaagccgc tgccatagaa aatgttatcg ccgagaacca tcgcgacgct
     6601 gtccttgccg atgaactttt caccgatgat aaacgcctga gcgagaccct cgggtctctc
     6661 ctgaccggca tatgagagct tcaaaccgaa ctgatgaccc gttccgagaa gtctttcgaa
     6721 tttcggcgtg tcgtccggtg ttgaaatgat gagaatttcc ctgatgcccg caagcatgag
     6781 agttgaaagc ggataataaa tcatcggttt gtcataaacc ggtaaaagct gcttactggt
     6841 aaccattgtc aaggggtaaa ggcgggttcc cgaaccgcct gcaagtatga ttcctttcat
     6901 tacttttaac ctccgtaatt atgtatcaat tattcgtgtt ttttaaaaag acttcccgaa
     6961 tggcagtcaa ttccgactat cagcggaaat ttatcgagta cgattctctt gaccgattca
     7021 cagccaagat caaaaaatgc gatttcttta cattccttaa cgcattttgc ggcaagtgca
     7081 ccggcgccgc cgacagcgca gagataaacg gcattattcc tgacaattgc atcgcaaact
     7141 tcattgtctc tgccgccctt tcctatcatc gccgccaatc ccttatcgag cagcaacgta
     7201 gcaaaaggat ccattctgcc agatgtagtc ggtccgcatg agcctatcgg cagtccctgc
     7261 ggcgcaggcg tgggacctgc gtaataaatg cacgctccgt caaggtcaaa cggcagctcg
     7321 ccgccctcat cgatgagctt gactattctt ttatgtgctg cgtctctcga ggtataaacc
     7381 actcccgaaa ggatgaccct gtccccggcg tgaagctcct ttgccctctc cttgagctcg
     7441 gacgcatcta aaacatatgc tgccattaac tcaactcccg aataagctgt tgaagcaatt
     7501 tttccgcttc ttgcggatcg gtttttacct tatcaagctt ttttgccgcc ttttttatct
     7561 gcgactgtgc gttcttttta agcttcgccg cagcagcttc ggcagactca accagcttat
     7621 cgcatcgctc tatggtttcc tgtgccgctt ttttatcggc ggattcctca agctgttttt
     7681 cgagcatctc atattttgct ctcattttct tagcgttttc ctcgg
//
LOCUS       DAWBSW010000093         7687 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1929_length_7687_cov_2.462526, whole genome shotgun sequence.
ACCESSION   DAWBSW010000093 DAWBSW010000000
VERSION     DAWBSW010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_7687_cov_2.462526"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6351..6360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1956 a         1862 c         1578 g         2281 t
ORIGIN      
        1 ggattaccac acgcccctca aacgtgcgcg tctgccgatt ccgccactct cgcattcaat
       61 tgtcgttccc tcaaggaacg cttgatatta tatcagattc atttttgttt gtcaactgtt
      121 tttgaaataa atttaggcct ttttcataat tattttttaa aaaccttccg acagcgatca
      181 agccgtcgga agtgatttta ttttgctgtc aaaaaacaat tttttatact cacggcgtaa
      241 ttgtatgccg ccttttattt ctcctcaaaa acatattcac ccgaaccgag gcgaataatc
      301 gcacagccgt ccttgcactc atactcatcg aagctgtgtt caacgccgtt tattgtgagc
      361 tttttgccca taatcggcaa tctcagctcg gtcggaaccg gaacggtaca tttatatata
      421 aggtttttct cgttgctcca ttcgctcacg ataagtcccg aggaggaatc atagcttgcc
      481 ttaacatatg tcatgcgctg ctgtccgtca ggaagctcct cggcggttct tgtatcaatt
      541 ctcggccgaa gaataacagt ttcaaagccc ggcattcccg gctcgatacc tgccatatag
      601 cgatacatcc attcaaccac cgagccgtaa gcatagtgat tgaatgagtt cataccgaca
      661 tctccgaagc cgttttcctt tgtataagaa ttccagcgtt cccaaacggt cgttgcgccc
      721 tggtcgatgc tgtaaatcca agacggctca ttgcgctgca taaggagcgt atatgccatt
      781 ttatccttac cgtattttga aagcgtcgtg caaagattat atgttccgag gaagcccgtc
      841 gaaagtctgt tgccgttcgc atttatctgc tcaacaagct cgtcggcgag aacttttgca
      901 tagtcggcat caacaaggtc aaaggcaagc gcaaggattt tatcggtttg ggtctgtccg
      961 atgagctttc cgtccttcat gtaattctca acaaagtaag cataagcctg ttttctcagc
     1021 ttcttataat gctcggctct gtcggattta ccgagaacgt cgctcatctt tatcataagg
     1081 tcaatatcgt gtacaaaata aacggacgaa acatacgttt tgtcgcactg atcatatgca
     1141 agccagtcac cgaagcgagc gtttgcgcct gccatgccga acttatcaac gagctgagca
     1201 atgtatttct ccattgattc ataatgagtt tcaagaagct caatatcttt atacataaca
     1261 tacatgttat aaggaacgat tatgcctgca tctgtccacg ccgcttcatt ctccgcatgg
     1321 cagcaaaacg ttttgggaat aacctcagta tatccgccgt cgtcaccctg agaatcccgg
     1381 gcatcctgga tccacttgcg gaaaaattcc ttgacatctg cattgtaagc agccgttacg
     1441 ctgaacgcct gcgcgtctcc tgtccagccg taacgctcat cacgctgagg gcaatcggtc
     1501 ggcacgctca gatagttact gcgttgaccc caaagagtat ttgaaataag cttgttgaca
     1561 agctcattgg aggtttcaag cgttcctatt tctttcgtaa cagagccgac aacttcggaa
     1621 ataaaatcaa gtatttcaat atccttcgtt gagcttatct gaacataacg atagccgtaa
     1681 aaagtaaatg aaggtctgta aacctcctca ttcttaccgc tgagtacata tgtttccttc
     1741 gcaagcgcgg ttctgaaatt gatggtataa agtgatcctt ttgcgccgtc atttcctctt
     1801 gaaatttcac cgctgtcgtt caaaaactcg gcatagtgaa cattaacaaa tgttcctctt
     1861 tcgcctttaa gggtcagctt agcccagccg accatttcct gaccgagatc aatgataagc
     1921 ttttgaccct ttttgagcgt tagaggaaaa gatttaggat tgtcgcagat atttatctcg
     1981 ccgtaatagg agccgttata attaacaccg tcataaaccg tgagagtttt tgctctcaga
     2041 cttaattcat cacgaacttg aaccttagga ccgataaaag gagtcaacac accgtcgaaa
     2101 taatcacaaa ccgtcgcctt gcgaaccttt ttcggcacaa atcctactgt tgaaatctca
     2161 tcataaccgt tctctgttgc atcacagtat tctccgtccc agatgtcagc caagcgaacc
     2221 ggtccgccga ccgttgcgtc ccagttttcg tctgttacaa gaattgattt gccgccatgc
     2281 acaatatttg caatgaaagc gggcggattt gcaccgtatg tactctgaac aattctgcct
     2341 gaataccagc ctgccgatac cactgcaagg attcgattct ttccctcatg aatatacttt
     2401 gaaacatcat agacgtagta aaaggcacgt ttgtgataat ttgtccagcc cggtttcatc
     2461 tcgtcattgc cgacacgctt gccgttaatg tagatatcaa caataccgag agccgaaaaa
     2521 atcaactcgg ttttctcgcc gcttacgata tcaaaatcac tccaaaacat cggtaatccg
     2581 ttattatcga caatttcgca atgctgatta acataaggca ctctgctgcc ccacggattt
     2641 tttgaagcgt caaaatagtt tcggtattct gtgtaaataa actttgcgtt attccatatt
     2701 ttttccatgt tgccctcctg taaaaaggac agaaacgaaa gcctttatta tgctcttgtt
     2761 tctgtcctct aatgtcttta attattttgt aaggaaatac caaatgatat caataactct
     2821 gaaatcctta agaaccttta caaggaactt aacgattgta tcggcaagga gagtcgttgc
     2881 tccgccgaga tcgacaaaac ggtttgtctc cgcctcgggt gcgccgtcat attcaagggt
     2941 gaacggctga gtatagataa ctccgccctc gcctacgatt tcggctctga catatgagcc
     3001 gatctcctct gaataatcat caaggtcaat catgttgccc gttgcaatta ccttgccgtc
     3061 ggcaatccag tgaaccatat atgcgtcccg agtgctgatc gtgatagtgt cctcaacatc
     3121 atcaacggaa acagccttta ccttaggagt aaccgcttcc tgatcaaaag caaaaatcgt
     3181 gcgctgcttg cctgcatttt cttcctcaac gatcttgctg taggtctcgg caaagctgtc
     3241 cgccagctca aggcccacac ctgccgcttt tagctcctcg gcccatgatc ggatttccgc
     3301 cttgctgccc atatagtagc ttgcggcaaa gaaagctccg tccttcatag cagttttaag
     3361 ctccgggatt gtcttttcag gcatacacat gagggtatag ccggaattga tgatgccctc
     3421 gttatgagaa tccgatgttg caagaccgaa aacgtttctg ccggtcggaa caaccttttg
     3481 aagcaggaga tcccaaagct ttctgtcgta acgggttctg tagtcgccct tgctgttcac
     3541 gtcgataccg agacatgccg ggtatttttc gagaatgttt gcaaacttat caactacata
     3601 cttgaactta acattatccg tgttataggc ctcatcatag ttggcaacat ttcttgcacc
     3661 gctgtactcg cccgggtgat tgattacgca gagtccgccg agtttatcaa cattcttaat
     3721 cggggtctca tagtcacttg taccgccgat tgtgtcgtta ccgtaattaa caaaccatga
     3781 gcaaacgtga gcattgttga acgaggtcgg gttctgctcg ttgccgaaag gcactctgag
     3841 catcgaattt tcggcatttc cgttctcgtc atactctgta taatagttgc cgtcgtatgt
     3901 atattcctta ccgtccgagg tctcgccctt tgacgaaaga acctcaatgg gaagcttgcc
     3961 ttttctgacg ctcattgcga ttttaacggc cttgtttgtc gagggatcgt cccagccgta
     4021 atccgttgtg ccatggtcgg tgattgcaag aatatcaaaa ttgtggtcat agtgcttttc
     4081 gacagtctct ttctttgttg atgtgccgtc cgtacccgtc gtgtgactgt gcagatccgt
     4141 cttgtattgc ttataggtgc tccagtcaac gttggcatag gtgctgctga ttttgtaatc
     4201 aacgtccttg tcgatcgcaa acgagcaaag agccgtacac gatgttgcaa ttgcggcaga
     4261 catgaaaatt gaaatggctt ttgtaagttt tctgttcatg attatcctcc gtattatttg
     4321 atgattttat cgagtagtgc ttttatatca atatctttat tctgcgtgtc ggaatttttc
     4381 tccgtcagcg gaacaatgat tttgttgacc tgatcgttat aaaggaactg cggaaaaaca
     4441 tcgctttcat ttaccgtcgg ctgactgcca tgctgagcaa gccaccatgt aaaatcataa
     4501 tagcccggcg tgtaccatgt gtgcatcata tctttgataa accatgtgta ttcgggataa
     4561 atacaggtcg atgcgtcaat ggcattgtcg caggaaatat ggttgtgacc gtcggcaacg
     4621 ccctgggtat agccctcggg gagcgtcgct ccgaaatcgg cacaggttgc gccgagcgag
     4681 gtcctcgccg tatctatcaa aaagtcactt tgataggcgt cgtttttcag caccggaacc
     4741 gctgccttac cgtaatttga gataattgca agcttcattc cgtgattcat ggcttcattg
     4801 agaatttcct ttgtgctgtc cataactccg tagtgatagg cgtcaagctt tttgataaga
     4861 gtcgcatttt cgacctcatc aagcatatat tcctttgcct gttcatagta ctcattgggg
     4921 acgaacgtcc atatgcccgt cagatttccg aaaagatctc tcagacagtc ctcataaagc
     4981 acatccttgc ctcttgtaaa caccgtatca agctcgccgc acacgggcga aagaagaaat
     5041 ccggaaagcg catagaaaat gcaacgataa aaagcagtca caggatcgct gccctcaatg
     5101 aaatttccga tataattcat tactgacttt gtttttatat tgaggtcacc cgtgaaaagt
     5161 ccgccgacaa gatttattcc gttaaaagcc gatgactgca ttattaccgt atcgacgctt
     5221 ccgtagccgt actgcttaag ataggtcata atgactgcgc cgcccatgct ctcgcccttg
     5281 agaaccactt tgtcgtggcg agtgagttcc ttagtctttt cgatatattc gttaagctcc
     5341 gcggcaattt caaaaacatc ctcacgccaa tcgtaattaa agcgattgtc acagcgatat
     5401 ccgtgctccg aggtcgggtc aaccctgaat ttgacgtcaa ccgtttcgtt gaggggatcg
     5461 ccgttatcgt cgcaggcaac gtccttcatc gtatcattga gaattttgct caaagagcct
     5521 gcaaatccct cataatcacc ggttattctg agcataaccg tcggaacgat aagcctcgca
     5581 acggcagata ctatcgccga ggtttcgggc atgaaaacgt tatattcctc ctcggttccg
     5641 ggatttgcaa ccaggtcggt catcgcaaat ccggtaacat aaacgatcgg cgtatgcgaa
     5701 cagccgcatt tgtctgttgc gtacgaagaa aatgatgcgg cactgaccga aaaagtcagc
     5761 aaaaccgcaa gaaaaacaga aaccgctttt ttcatagaag tcctccccgg tttgttatca
     5821 taataaaaat cttaggcaga tttcatacac ccacggccgg caatattcgc accgtaccgt
     5881 aaatgttttc ggaaataccg aaaaactgtc cgcattgtcc catttccgat ttcttttgta
     5941 ttatatcata agtttttatc aatatcaatt cttattttat tgaaattgtc ataatcattt
     6001 atacggagtg atgctgatga caaacgcaaa aaagctgctt acaaatacac ttctgctgac
     6061 ggctgcggca ttcctgatga aaacggtcgc tgtcagcttt aacgtttatc tgacaaataa
     6121 aataggcgca gacggaatcg gtctttttca gttaatcacc gccgtttacg gcatgtcaat
     6181 aaccttttcc gttgccggaa tacgccttgc cgctatgaga atcgttgccg acaatatagc
     6241 tctcggaaaa tataacgaca gacagataat gcgccgctgt cttgtttatt catttatctg
     6301 cggcaccgtt attgccgccc ttttggcagg cttttcggaa tttatcggtt nnnnnnnnnn
     6361 cgacgtgcga agcgttccgt cgcttaaagt actcagcctg agtttgccgt tcgttgcgat
     6421 gtcggcagcg cttaacggat attttacatc ggtaaagaag atcgtccgct atactgccgt
     6481 tcagctttcc gaacaggttt tcaagattgt tgtaactgtt tttttactga aatcggttat
     6541 aactcacgga cttgaagcct catgtacagc aatagttttc ggcatttcct cggcggagtt
     6601 tttttcactt gtctgctcat atacgcttta tcgcttttcc gtatgcaagc caaaagagac
     6661 ggaaaacggg cgtgacattt ggaaaagaat tctaaagatc tcaattcccg atactgtagg
     6721 ctcggagatg cgttcaattc ttatgaccgt cgagcacctg ctcatccctg tcggcttccg
     6781 caaatccggc tgcgatccgc agacggcgct tgcgaactat ggtgttattc acggaatgtc
     6841 gcttccgctc gttctctatc cctccgccct gctctcgtcg ctctccggac tgcttgtgcc
     6901 cgagatatcc gctctgcaca tttcggggaa caagccgaga ataaactaca tgatagcccg
     6961 agtccttcat ctgacgctga ttttttcaat cggcactgca ggaataatgt attttaacgc
     7021 aaaaaggctg tcacttgcgg tttatggcaa tgacagcgct ttcttatata ttcaaattct
     7081 ttcgcccttg attcctgtta tgtattgcga tatgtccgtt gacggaatgt tgaaagggct
     7141 tgaccagcag gtcagctata tgagatacaa tattatcgac gcttcaatat gcgtcgttct
     7201 cgtttacctg ctggttccga ttttctccgt aaagggttac gtcatcgttg tattcgtcag
     7261 tgagataatc aattttattc tcagcttcag acggctgaca aaggtcagcg acgttaacat
     7321 ctcttttttc aaagatattt tcatcccgat tttttgcatt atcggtgcaa atctttcaaa
     7381 aaactttatt ttcttctttt tcaacataac taatttcaaa cttgccgctt cccttgaaat
     7441 tattgcggca gttaacctgt acaccggaat gatagttctt tttcgttcca tagacaagga
     7501 agaacttgat tggatgaaat ctcttgtcaa ataaaacgtt tttcgatata catttttatt
     7561 tttcccatct tatattcaag tttttcacaa aacctgttta caccaatcag gcagaaaagt
     7621 tttctctgcg ctattcctat caatgagcat ccgagcgtta ccgcaagtga gataccgatg
     7681 acaacaa
//
LOCUS       DAWBSW010000094         7603 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1952_length_7603_cov_3.179915, whole genome shotgun sequence.
ACCESSION   DAWBSW010000094 DAWBSW010000000
VERSION     DAWBSW010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1952_length_7603_cov_3.179915"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1964 a         1841 c         1642 g         2156 t
ORIGIN      
        1 gcaccgagac gaatatgaag atcgaagtca aaacctgaat taaatatgtt cttgccgaga
       61 aggccagctt cacgggcttc cttgatagca acatcaagac gatgaacggc aataggatac
      121 tccgcacgaa cgtaaacata tccctcggtt gcacctgttg cgtaaccgca gatcgtcata
      181 gcctcaacga tacaatgggg gtcgccctca agaacggaac ggtccatgaa tgcgcccggg
      241 tcaccttcgt cggcgttaca tacaacgtac ttctggtcgg ctgtattctt agcggtgaag
      301 ctccacttaa gacctgtggg gaatccgccg ccgccacggc ctctgagtcc ggaatcctta
      361 acgatctgga taacctcttc gggcgtaagc tcggtcaagc actttgcaag agcctgatag
      421 ccgtccatag cgatatattc ttcaatattt tcagggtcaa taacaccgca gttacgaagc
      481 gccactctgt gctgcttctt gtagaaattg gtttcattca gcggcttgat atcatcctga
      541 tgaactgtct cttcatagag aagtctcttg accatacgac ccttgagaag atgctcctcg
      601 acgatttcgg gaacgtcctc gaccttaacc tcgctgtaga aacagccctc gggatatacg
      661 atcatgaccg gacctaatgc gcaaagtccg aagcagccgg ttttgattac tttaacctcg
      721 ttttcgagac ccttggcttt aagctcctgc tcaagctttt caatgatctc ggcactgtgt
      781 gaggaagtac agccggtacc gccgcagaca agaacgtgtg aacgatacat tgtttttcct
      841 ccttaatctt ttactacagc gccaacggtg tattcaacaa ccggattgcc gtttacaata
      901 tgggaagcaa cgatttcggc aaccttttcg ggacttacct gaacataggt taccttctcc
      961 ttgccgggtt caaaaacctc aacgatcggc tcatactggc acataccgat gcagcctgtc
     1021 tgagcaaccg taacgttttt aagatttctt tttgcgacct cgtcaacgaa tgcgttgaga
     1081 acgggacgtg cgcctgctgc gattccgcag gttgccatgc cgactacgac acggattccg
     1141 tcgcctgtac cctcacggtc tgtcattgct gccttagcct tatcacggat ggcaagcaac
     1201 tcttcaagtg atttcattta cagtacacct ccacggatag tttgtgtttg ttcatgcagg
     1261 tactctttaa tccactgagt aaccgacggc tcattaagcg gcacgtcacc gagtattcct
     1321 ttaagctcgg aagtggaaag agtaaattct ttttcatcga ccttcagtcg aaagataaaa
     1381 ttacggtctg tgttcatggt gatgagcatg ataacagtgg aatcaatatc gccgagcggt
     1441 gtgtaatcaa tgctgtcggt cttaaaaatc gccttgacct ttgttccgac gccgacctcg
     1501 gattcgatgc agaggcttcc gcccgtctgc tccgccgcca gcttaaacag aggaattccc
     1561 atgccgacct ttcttgtcgt tctggtcgta aagaacgggt cctgaacatt tctgacctgt
     1621 tccggggtca ttcccttgcc gttatcatag attcctatca gcaactcgtt tttctttgtg
     1681 tccccgacaa tttcaatctc aataagcgaa gcgttggcag aaatggaatt ctgcgcaata
     1741 tcgagaacat tcaatgacag ctcacgcatg atttttctcc gtaattatgc tctgtatttg
     1801 gcaagaatgc cgggtacatc gtctgcaaca agctttccgt aaacctcatc gttgacggtg
     1861 agaacggggg caagaccgca cgcgccgata caacggcaag cctcaaccga gaacataccg
     1921 tcgttcgaac attcgcctgc tccgattcca agctcctcac aaatcttgtc aagaagttcc
     1981 tgcgaaccct taacatagca agccgtaccg agacaaacag agatgttgta ctctcccttc
     2041 ggggaaagcg caaactgagc gtaaaaagtg gatactccgt aaaccttttc aagcggtaca
     2101 ttcataccgt cggcaatcat ggcctgcact tcaaacggaa ggtatccgta tatagcctgc
     2161 gcctgctgca taacgtgcat gagcaagctt ttattgtcct tgcactcctc aattactgct
     2221 ttaagctgtg cctcctgctc aggtgttcct ttgaacggga ttttgctgat ttttttgagc
     2281 atttaagttg ttcctccttt acaacagttt agccgtatgc gaacggtcgg aacggaataa
     2341 aaacagtctg ttaaaatatt aacactccga aagcccacaa tacaacatta taatgctgaa
     2401 acattatatc acataaaaaa taaatagtaa acacatttat cgaattaaat tctgccaaaa
     2461 cggctcaaaa tcgacaattt ttcatttttt tgcctgtttt tttcctacaa attttatctt
     2521 gtacgaaaat tgctataata aataagtcat ttattgaata actctttatc taatatattt
     2581 ttatctatat cgcggtatac ggatctgttt tgtgcataaa agtaaaccgc ccgaactgaa
     2641 atgcaattca tttcaagtca agcggttatg aaatcaattt attttatttc ttcgcttttg
     2701 tcggtttctt ttcgggaagc gtcacaacat acgcaagctc ccaaagagca attccgataa
     2761 agaaaatcaa gctgagcgtt actgcatttc cgagtgtgaa cgaatcgacc gagattgcac
     2821 ccttgagaat cgacgtgcct atcgaaccga gcagattgtt actctctccg acaaaggctt
     2881 tcgcaagtat ctcgccgatg tcaaccgaac cgtttatgta cggagaagaa aacccggcaa
     2941 agcatctgag agcagcaaag cagcaaaccg ctccgcctgc tccgagcgca gtaatggtct
     3001 tgtaagcgtt cgtgaaaagc gcacagccga taataactat cgcaatgatc aacgcaatta
     3061 caatcaatat ccccgcggca atcgcccaat tcttgcctga gagaagctcg gcaggtatct
     3121 ttgaaatgtc gaatttcatt ccgccgaatg aaaagctgcc gtcgctgatg tacttgaaaa
     3181 tctccttaac ggaaaaggtg atctccatag cactgcttgc cgttttctcg gcgagctttg
     3241 agagtattgt atacagcgac gaatcctcgg aaattgaaac aacagctctg acaatattca
     3301 taaaatacat aataggaaaa atcgcaagag tcaccaaaca aactacgatt ttgttgaata
     3361 ttttcatcgt tttcagctcc tttatttgcc ccatgaagag ttaagagcaa cggtgcgatt
     3421 gaatacaagc ttgtcctcgg tgctgtcttt atcaacgcaa aaatatccgt ttctcatgaa
     3481 ctggaacgtg tcaccgggct ttgcgttttc aaatccgccc tcaagcaggc aattttcaat
     3541 aacctcaagt gagtcggggt tgagatcgtc aaaatagtcg ccgttcttac cgggctcctc
     3601 ggcgctgaaa agtctgtcat aaaggcgaac ctcacattgc ttacagtcgt tggcattaac
     3661 ccagtgaatt gtgcccttga ccttgcggcc gtcgggggaa tttccgcctc gactctcggg
     3721 atcataagta cagtgcaggc atgtaacgtt gccgttttca tccgtgtcgt agctattgca
     3781 cttaataaaa tatgcgctct tgaagcgaac ctcgttgccc ggaaaaagtc tgaagtattt
     3841 cttgaccggt tcagccataa agtcatcctg ctcaacatag agttcacggg agaaagtgac
     3901 ctttcttgtt ccgaactccg ggtgctcggg gtgaagctca acctcaaaat cctcactctg
     3961 tccttcggga taattatcaa taacaacctt aagcgggcgc agaacagcca ttgcacgagg
     4021 agccttagcg ttgagatcgt ctcttacgca agcttcaagc aatccgtagt caacagtgct
     4081 gtccgccttt gaaacgccta ccctgtcgat aaaatccctg actgcggcag cgctgtagcc
     4141 tcgtcttctc atgccgcaaa gagttatcat tctcgggtcg tcccagccgg aaacgatacc
     4201 cttgttaacc atttcaagca gctttctctt gctggtaaca caataattta tgttaaggcg
     4261 tgcaaactca atctgtctgg gcggttcgtc aaagccgatc tggtcaacga cccagttgta
     4321 aagaggtctg tgattttcaa actcaagcga gcaaagcgag aatgttacac cctctttggc
     4381 atccgaaagc ggatgagcga aatcatacat cggataaaca caccacttgt caccggtctg
     4441 gtgatgtgaa acatgagaaa ttctgtagat aacgggatct cgcatattaa gattcggcga
     4501 tgacatatcg atcttagctc gaagaacctt ctcgccgttg ccgaattcac cgtccgtcat
     4561 acgcttgaag aggtcgagat tctcctcaac gctcctgtta cggtagggac tttccctgcc
     4621 cggctcggtg agtgtgcctc tgtattctct tatctcgtct gcgctgaggt catcaacata
     4681 agccttgccg tccttgataa gcttgattgc gcaatcatag acaaaatcaa aatagcttga
     4741 agcaaagaac acgttatccc agtggaaacc gagccacttg atatcctcct tgattgcgtc
     4801 gatatattca acgtcctcac gggatgggtt ggtgtcgtca aagcgaagat tgcagattcc
     4861 gttgtatttt tccgccgtgc tgaaatctat gcaaagggcc ttcgcatgac cgatgtgaag
     4921 atatccgttc ggctctggcg gaaatcttgt ctgaacacgg gatttcactc cgcttgcaag
     4981 atcctcgtcg ataaagtcat gtataaaatt tgatgaaatt ttcttttctt ccatggtgac
     5041 ctccgattat ttgtatgatt tggctgcttc aatgagtctc gccttaactc tttcattgcc
     5101 gaggagagca attattgaat gaagatccgg cgtgttgcgg cgagaggtta tcgcaattct
     5161 gataaccgtc gaaacatcgc cgacatgacc cttgaaagcg tcggggttct ttttatattc
     5221 cttaacgttc ggagtatagc caagcggctc gcaaatctcc ttgatctttg caaaccaggt
     5281 atctttatca tccgatgcgt caaaaatctc agtatatttt tcaaggatag caacagcgtc
     5341 atccctgctt atgttctccg ggagcgtgta atccggtgtg taaatctcgt catagaaata
     5401 cgaaacataa tcctttacct cgtcccactt tgcgatatcc ttacggggct ttgcagttcc
     5461 cctgtcgatt gagaaaatgc cctttgcaaa ctcggcattt ccgctgagaa gcgcataaag
     5521 ctccggatcg ttatcctttg cccataccgc tgccttgtcg taaacctcgt cggcagtcat
     5581 aagtgaaata acattcttgc tgacatcatt aagcttattg aggtcaaaca gtgctccgct
     5641 tgcgctcatc tttttcaggt tgaacgggaa ggaactctgc ggcgcagtct tgtttgcttt
     5701 gcgccaatct tcaaagttgg agtttgctat tgtcagaaga tattcgataa cactctcctt
     5761 cggatatccg ctctcggaat agaacgaaac ggaagcctcg ggatccttac gctttgaaag
     5821 ctttctcttg ccgccgttgt cctctttcat gataggggaa atatgagcat acttgggcac
     5881 cttgaagccg caagccttga aaagctgaat atggagcggc acggaagaaa tccactcgtc
     5941 acctctgaca acatgagtcg ttctcataaa atggtcgtca acggcatgtg cgaaatgata
     6001 ggtcggaatt ccgtccgtct tgagaagaac aacgtcctgt atattctcgg gcatttcaat
     6061 cttacccttt atcatgtcgt caaacttgat tctgtgatct gcctgtcccg gggagcgaag
     6121 tcgcagagta taggggcagc ctgccttaat tttctcctcc tgctgttcaa gagtgaggtt
     6181 gcggcacttt gcccattctc caaagtagcc ctgaatatcc gttccggctt tttcctgctc
     6241 ggcacggatt ccgtttagct cctcctccga gcagaagcac ggatacgcca tgccctgacg
     6301 aacaagcgac tttgcgatag cttggtaaat gtccttgcgt ttactttgct gatacggtcc
     6361 gtatgctccg ctctccgact caaagcccat tacgccctca tcgggagtta caccgaaatc
     6421 gtcaagaccc ttgataattg cagaaacgcc gtcctcaatc tcacgcttct tatccgtgtc
     6481 ctctattctg aggaagaaaa cgccgtcgtc ggtcgcacgg gtcgtgagct ttgaaatcat
     6541 tgcggcaaaa agtccgccaa gatgaagaaa gcctgtcggg ctgggagcaa atcttgaaac
     6601 tcttgctcca tcggagagac ctctctcggg atatattttt tcatagtcct cgggtgtttt
     6661 tgttatattc ggaaacaaaa gctcggcgag tttttcgtta tctgtcatta caaggtcccc
     6721 ttttaacagt catataaata cataatacat tatcgcagaa aaagcgtgca gtgtcaaggt
     6781 ttagcgcagc tttttatttg acaagttgtc acaaaatcag gattaaaact cattgacaat
     6841 tgtttttgac aatgctatac tttttatgtt cactcaggga gcgattgttc agtaaaagcg
     6901 aaagcctaac agggtgcaga ctgagaaagc tgtatcttgt tcggcttttt gtatgtataa
     6961 ttgttttgtg atacgatttt taaaaaacgg acacagaaac aacgtaaacc attttcagga
     7021 gaataaaaaa tgacaaagga tgaatattta ataacagata cgccactcaa ggcgttgact
     7081 gtttttgcga tgcctatgat tttaggcagc ttctttcaac aagtatacaa tatggcagat
     7141 tcaatcatag tcggtcaatt tgtcggttcc tctgcacttg cagccgttgg tgcttgtgct
     7201 gcacttacga atgtttttat ttgtatagca ctcggcgcag gcgtaggtgc aggtgtactg
     7261 gtcagccgtt atttcggtgc tcataattac ggtaaaatga aaacaatcgt atccacttcg
     7321 ttaatcagct ttttacctct aagtatattt ctcggagttt ttgggttttg cagctctcat
     7381 tggatgatga gcatactgca aacgcctgca gatataatgg aggatgcggt gctgtatttt
     7441 cgtatctatt ttgccggctt tccgtttttg tttatgtaca acattctgtc aaccatgttt
     7501 acttcaatcg gtgaatcaaa aattccgtta tggcttttaa tcttttcatc tgttttaaat
     7561 atcattatgg atctttggat gttaggcggt cttaaactcg gcg
//
LOCUS       DAWBSW010000095         7587 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1955_length_7587_cov_2.427377, whole genome shotgun sequence.
ACCESSION   DAWBSW010000095 DAWBSW010000000
VERSION     DAWBSW010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1955_length_7587_cov_2.427377"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4576..4585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2133 a         1636 c         1797 g         2011 t
ORIGIN      
        1 catgaaacgt aatcgctctc gtcgtcatcg gcaacctcgt tcttgcaggg aagaaccttt
       61 gttacgagat aataaacgcc tgcaacagcg ccggcgatag caacaagaat gccgactacc
      121 ttaaaaatct tctttataac atcgcacata attaaaacct ccaaatgttt tattttccta
      181 attatagtat aagaacaata caaagtcaag cctttttgaa ataaattcat ggtttataca
      241 caaatgaatt tatatgtaaa aaacaaaagg acggaaaaat ccgtccgtca tgtttattca
      301 gattagtctg cgagaagctt tgaaagagcc gacttctttc tggcagcgtt gttcttgtga
      361 ataaggttct ttgcgcaagc ctgatctacc tttttgatag cggctctgac aactgcttcc
      421 ttatctgctg cgtttgtttc agcagcaacg tgtgcctttt tgatagcggt tttcattgca
      481 gaatttcttg acttgttgcg tgcagccttg gtctggttaa caagcactct tttcttagct
      541 gatttaatgt ttgccattgt tgcacctcct ttaataaatg cattacacag ttagttatga
      601 tatcattaaa cagtgaaaaa tgcaagtgtt ttttgatatt ttatttaaaa aaacggataa
      661 tttttcaaat ataccgattg gagcgataaa agatggaacc aagaaccgat cttgcactcg
      721 aaaaacggga gatactcggc aataaagagc ccgagggagt ggaaagctgc gaatttaccg
      781 agggtgaagt aaatttcacc aaaataagaa ttctcaacga aaagggaagt gaagctctcg
      841 gaaagcccgt cggcacctat attaccgccg agattcctca gttgaccaaa aatccgataa
      901 acggtgagga gacgattgaa gcaattgcaa aacagctcag gtcacttctt cccgatgacg
      961 gagcggtgct tgtggcaggc ttgggaaaca ccgatattac acccgacgcc gtaggaccga
     1021 aaagcgtttc gatggtgctt gccacacgtc atattgataa atcgctgtcc gaggagatag
     1081 gtcttggcaa gctcagaagc gttgccggct ttgtgcccgg tgtgcttggc agaacgggac
     1141 ttgaggcggc ggaatcggtg aaaggcatcg taaattccgt ttctccgtca gccgtgatag
     1201 ttgttgatgc ccttgccgca aggaggcttt cgagactcgg aactacagtt cagctctccg
     1261 acacgggaat aattccgggc tcaggagtcg gcaatgcaag gaaagaaata accgaacgct
     1321 ccgtcgggat tcccgtcatt tcggtaggta ttccgactgt tgttgatgtc ggaacgcttg
     1381 taaatgacct gaccggctca aaaagcgata ttccgcaggc taaccgaaac atgataatca
     1441 cgccgaggga aattgatatt gtgattgaac gcgcatcgga gctgatcgga atggcaataa
     1501 ataaagcgct tcagcctgat atatccgttg atgaaatgat gatgattgtc ggaaactgaa
     1561 attatcttat ttgtgaaata taaccggttt taattgcgta ttttttgaca gtctcgatat
     1621 cggtatcgga aacgctgccg tcaaaatcaa catccgctgc gaaagttaca cagttgtcat
     1681 attcgcccat gccgttagtc atgcagccca ttattacggc gtccatgcca tcggctttac
     1741 agtccccgtt aacgtcgccg aaaataataa tcgtaaacgt cttgtaaagc tcccctttca
     1801 gcgtatatat tttgagctgt gagcctgttc cgttgccgtt ggcggtttgg actaactcag
     1861 cagtgcctgt gccgtccttg acggtgacat agcttgcaat gtctgcaaag ccctcgtcga
     1921 gtccgtagat atattctttt gacgaatcgt gaatcaccgt cgtgttgaga ttgaattcta
     1981 tttcgggctt tgttgctccg aagtcaagaa actttacagc gtcgagcatt ttgtatgtat
     2041 aggtggtgtc ggttgaaacc gtcggatttg aagtcgctgc cggtcccgaa aattctctga
     2101 gagagttaaa aatcttgtca acggtttgtt cgggataaac cgaagcgtac aacgcaatgc
     2161 acgcagcaac aaggggcgtg gcctgagaag taccgctcat aacggaaaac cgtccggctt
     2221 caattccgca tcccactatt gcctcgcccg gagcaacaat atcataatac tgaccgttgt
     2281 tttggtcata gtttgagaac gtggtaagct cggacgtgct tgtcttgctg cttgccatta
     2341 cgccgataac gttgcttgcc gcggcagggt aggcgagcaa cgacgatgtt cccgtgccgt
     2401 tgtttcccgc cgccgcaaca atgcatatgc tgtccgcata agcgtcgtca acggcgttct
     2461 tgattgccgt cgggtaggcg ttattgattc cgagcgacat actgataaca tcggcaccgt
     2521 taacgcgtgc ataaactatt gcatttgcaa cggcggagct tgttattctg gttttgacgt
     2581 cggaagcggt gtaattgtct tccgctacct tgataagcat gagctcggcg ccgaaagccg
     2641 ctccgacaaa attgcttccg tttgaagcca ttccgataat tccggcaaca tttgatccgt
     2701 gtgcactgtc gttgagggca gaaccgtcgg gacgcagagc agggctgatg tcgtaattgc
     2761 cacttgccgt atttctgccg tgattgccgt ttccgtcgtc ccaaagattg gtcgggaagt
     2821 cggccgcatc aatgtagaag ccgttatcaa tgaccgcgac tgtcacgccc tcaccgcagg
     2881 tttggtattt ttcccacgcc gcaggaatgt tcatgacggt atcgaggtac catttttcat
     2941 aatttgtata attagctata ccgtttgtta tttccaaagg tgctgtatag ccgtaggttt
     3001 caagcagata attcggctca gcatatctga cgccgctgat cttttcaatc tcacggcagg
     3061 ttttttcaac gtcaccgctg atttttgcaa catatgttgt cgattccgag gaaaccgagc
     3121 ttgtatgaat tgcgtcatca taattatcca gcgctttcag ctccgttact ccgagagccc
     3181 tgagcattta acggaaagcc gcaggcgcac gccgcacacc gcccgaggat atcggcggag
     3241 cgtactcgaa tacgacctcg tcctcaacat aatcggctgt tatgatttcc tttgccgaaa
     3301 cggagaacgg caaacatgtg aaaaccgtaa cggcagtcag caatacgcaa ataattctct
     3361 tgattttcat aatatcactt ccttcggtaa aaatagtatg gggtgattat attgaaaaaa
     3421 ataatatcaa taattatatc gggaataatg cttctgctta tgtgctcgtg cggagccgat
     3481 atgcccgacg acatgacaaa caaagttaca acggcggaaa atgtcgccgg aaaaacaggg
     3541 cagaaagcgg cggcaaccta tacgggttat cctgcgcttg agccggtgac gctcacctgt
     3601 gcggatcccg agaattcaag agggctttcg actgcgaaaa tatcacattc atacggtgtc
     3661 gccaaggacg gaaagcctca tcaaatctcc gttgactcgg aaaaattttt tgagagtaaa
     3721 aatttcaagg ctgtgactta tgacaccaag tcgcaaagca aggtactcta tctgaccttt
     3781 gactgcggct acgataacgg ctacacggag aaaattctcg atactcttaa ggagaagaag
     3841 gtaaaggctg cgttcttttg caccgccgat gagctgaaat ccgctcccga ggtcattgca
     3901 agaatgataa aggaaggcca tattgtcgga aatcattcga caacacatcc gagctttgat
     3961 gaaatcagcc gcagtgagat ggcaaaggaa attcaggact gcgataatta tctgcgctct
     4021 aatttcgggt acacatcgcc gtatttccga tttcctaaag gggaatactc ggaatgtgcg
     4081 ctcgagcttg taggctctct cggatatacg agtgtattct ggtcgctctc atatgccgac
     4141 tgggacacaa aagcgcaaaa gggcgcagat tatgcttttg aaaaagtaac ggcacgtctg
     4201 caccccggtg cgataatcct tttgcacgcc gtttcgtctg acaatgcggg agcaatggca
     4261 cggattatag actatgcgag ggaacagggc tatgaattca aaagtcttga cgatttgaaa
     4321 ttataattat acaaagtaat tatatcataa gtcgaattcc gttacaacat ttttttaaaa
     4381 tcttaacatc ttgcaattaa agctgtgaaa tgctataatt attccattaa acacatcgga
     4441 ggaatagatt atggcttcac agctttccat gcccacgaag ggcgacatga ttgcaattat
     4501 gaaaacaaac aggggtacaa tcaagttcag acttttcccg aatgagtgcc cgaaaacggt
     4561 cgaaaacttc acgacnnnnn nnnnncaaac ggcagccagt tctttatcgt tcaggcaggc
     4621 acggttgacg gcaacctcat gggtcagatg gaaatgatcg gcgaaaagat gttcccggct
     4681 cagtgtatcg aggactacaa aaaggtcggc ggaactccgt ggcttgattt taagcacacc
     4741 gttttcggtc aggcttatga cggaatggac gttgttgatg aaattgccaa gacaagggtt
     4801 gacttaatgg acaaaccgtt tgatgacgtt gttatcgaga aaatcgagat cggcgagtac
     4861 gagggctgag gaagcttttc acgggcaaaa cggcaggaat cttttctcgg cgcccctgta
     4921 ggggaggcaa gcccacatcg acccaaggtc agatgaaacc gtaatggacg cccatttagg
     4981 ggagctgtca gcgaagccga ctgaggggtt ttatgaatat agtctgatga aatttaaata
     5041 acccctccga cttccgaaaa ggaggagaaa aaatgaaata tattaaatgg ctgatagcgt
     5101 cggttgttgt tgtcgctttg ctgatattta ccgcagtgtt tgttatcaat gcttataata
     5161 cgggcaggat aggcaataag gacgaaaagg ttgccaacga atttatcgag gaaatacagg
     5221 gagtttggaa gaatacctca actggtaccg tcgcttcaaa ggtcagcacc gtccgcttcg
     5281 gcgaggacgg aagactgacg gttattgttc tcggtcagac ggtaggcgga gcatattccg
     5341 acgaatatga ccttgattca aaaaagcata tattgacggt caaaggtaat atttacggcg
     5401 gactttcaat tgaacgctca tttgaagcgt cgctgaacga ggataaggat acacttacac
     5461 tcaaggacac aaagggctcg ttcgatttta cacttgtaaa aaccgatgaa accgaattga
     5521 caacggaaaa aacgacgcag aagaaagaaa cgactgtccg gaaaacggat aaaccgaccg
     5581 caccgacggg cgatacggcg aaatatgccg aagctctgct cggcaaatgg gtatcaaggc
     5641 tcaacacggc atcgggatac gaatttgttg actattcgac ggtcaggatt tcgcttgcag
     5701 gtatctcaac cgacggaacg tacacgcttt ctctcaacga aaacggtcgg tgcgaggtaa
     5761 aaataaatta tgtgagcctg gcaggaatga ccgtatccaa cacatacatt gccgaaatcg
     5821 gagaaacgga gcttactctt attcaaaaga atgccgaatc cgtgtcggtt acctatgtta
     5881 aagcacagta aaacggtgtg ttaacaaaaa atttataaat atcttcattt ttctacttgt
     5941 tatattcccc gttttggtat ataataaaat gaaactaagc ggaggtgttt gcaatgacgg
     6001 ataaaacgat tcagttttcc ataaaggaag accatgagaa ggaaatgaaa tcgatattaa
     6061 aagccgttta taatgcgctg agtgagaagg gctacaatcc caccagtcag atcgtaggct
     6121 atcttctttc cgaggatccg acatatatca ccactcacaa taacgcaaga agtcttatcc
     6181 gaaggataga ccgtgatgag ttgctccagt ccttggttaa aaattatctc aaagattaag
     6241 aaaggctgat ataaatggca gttaagaaaa agggagatca gtttctcacc tacagaggca
     6301 agcctcttgt tcgaagcgga aacatgatct attacggaaa catgaatgac ccgtacgtta
     6361 ttatgattca gattatgagc aaaaagatgc agggggatat tgaggttccg gacagggtcc
     6421 tcattcagct cctcaacaca gatcccgatt gccgtccaaa ggagcgcata gttaagaaaa
     6481 gcgaaaagca cggactttac aatgcgatgg atatcggaac gatctggctt gaaagagcgc
     6541 tcaaataaac atagcaggat taacggactg tacccaatcg gcacggtccg tttttgcgaa
     6601 ttttgacgtt tttaacaaaa gcatgctatt gtcttaatgt taacaaaagt tgatttttat
     6661 ttagtggact ttattgaaaa gatatgttat aatatcaaat taggtaagcg aattatctgt
     6721 aacggaatat atgggagggg cagcaggtgc aaaagattga tgccaccgta aaaaaagaaa
     6781 cgcttttcat tgccgctttc actttgatat tcagtgcggt catggagctt gttttccttc
     6841 ttctcaatta ttggagctat aaggttgtgc ttggcaattt gctcggcttt accgcggctg
     6901 tgctaaactt tttcctgatg ggctatacgg ttcagaaggc tgttctcctt gaagagaagg
     6961 acgcaaagaa aaaggtgaag ctgtcgcagt ccctgcgaac actcatgctt gtggcttttg
     7021 cagccatagg ttgcgttttc aagtgcttta accccattgc ggttctttta cccttgcttt
     7081 tcccgagaat cgctatcctt ttcagaccgt tattcaaaaa taacgattga tatataattc
     7141 atacacggag gtgttgccga atgaataaaa gaagcatagg cttcaggctt gttgatgcct
     7201 tgctgatact tatgatgatt ctgccgctcg tcggcgcaat ggcattgcag gttttgacca
     7261 agcccgcttc ggacggcgtt gcaattgccg gcgcaagaat tttctttaca gttcatatgc
     7321 cgatacagga tctcccgata acggaaagtc aggtcaacac atggcttatt atgatttctc
     7381 ttttcttcct ctgtctcttc ttcacgcacg gcttgaccga agaatgcagc tcgaaacggc
     7441 aggcaatagc tgaatggata gtagaaaaga ccgatgacct tgtaaaggac aacatgggcg
     7501 aatatttcaa ggagttttct ccgttcgtct gcggaattat ggcgctttcg gccttttcaa
     7561 gcctttcgtc aatgctcgga atttttc
//
LOCUS       DAWBSW010000096         7552 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1968_length_7552_cov_2.386688, whole genome shotgun sequence.
ACCESSION   DAWBSW010000096 DAWBSW010000000
VERSION     DAWBSW010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1968_length_7552_cov_2.386688"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1069..1078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2096..2105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2783..2792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6165..6174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2077 a         1695 c         1537 g         2203 t
ORIGIN      
        1 gctatggctc aggtggtaga gcacatcctt ggtaaggatg aggtcaccgg ttcgagtccg
       61 gttagcagct ccaaaaggca ggtttttacc tgtctttttg ttttgtccaa attaagctga
      121 ttttcctcga aacaacgtaa aatcaacgtt tttggcattt tgcagaaaaa ccgacttaaa
      181 ccgaaattaa cccaaaatca accgtattgc agtcactatt gcagtcattt tcacaccccc
      241 ataagcgcaa aaaatccccg agctaccaca caagtggtag ttcggggata agtttatttt
      301 agcaagttac cgtcaaatta cgggcaagtt aaaatacctc ataccagtca tcggaaagga
      361 tatcggactg agaggcgagc cagcccgtga ggacggcagt tctgccggtg ctgtcatgtg
      421 tgaacattct aattgagccg agacagttca attcattctc accatctccg aagcactcaa
      481 gaagtctttc gtcacggcac cactctcgct ttaccatcgc cggaggaaga agccaaatat
      541 acattccttt gccattccat ccggttcgtg cgaccttttt gccttctttg agcttacgga
      601 tcgcctcact gaagctgaat gtctcatatt cctttctggc cggatattct gcatttttcc
      661 cgtaattctt ctctcatata gtaaagcggt acctgatttt gcacataatc cgggtaccgc
      721 tttgccatat atatattata taaatttccc tacaaattta atctccgctt ttattatgga
      781 taatcataat aaacaacggt ttccgaaccg gcagtaaagg ttaccttcaa cctgtagctg
      841 gcaagaacat ttatgagtcg ttcttcttcc attgtcagga ccattccggt tcttgatgcg
      901 gtccatgttt ctattgtctg atatatgccg ctctttttct tctgcaattc cattttgatg
      961 tgaaggtaca ttgaaccctt ggcatgaagg accgcagatg ataccgagtt tacgcccgag
     1021 agtttcagcg agcatgaatt ggccgaaata ttttcatatg ccggctcann nnnnnnnngt
     1081 gccatagcgg ccgtggagcc gcacattatc agacacatag caacgcaaag aatttttttc
     1141 agaacttttc ttatcatatg ataagcctcc tttaatagta tttgtatata tatgagcttt
     1201 tccctgcttt gtgacaaaaa tttttgccga ttttttcttt tctctgtttt gcacaaaaag
     1261 cctttttata attgttagtt ttcaccaatg tgccacgcga tttccttcaa aacctgactt
     1321 gaaatattgc cggaaactgt gtattgatat ccgttttttt cccaaataat tccgtatccg
     1381 tgccgggttt ctccatattc aatatagtca attccgttta tttgcgttat tttgcagtcc
     1441 ttgtattcgg tattgatgtt tatcccttca ttacaagctt ttttagtgac ggtaaaaaaa
     1501 tcttttcctt ttacatattc ttttgaatgt tcatatttat tatcgctttc aaagctcaag
     1561 gcaaacccgc ttggaataaa gcccaattta aaattatcta ccatcttgct gtccgactgt
     1621 tcaaacagta cggttgaatg atccgaaaaa ttaaagatat tatatttata ttgtgcatac
     1681 cctattgatc ctattgctaa cataaccagt attatcgcag cgataagaag tattcttagt
     1741 ggcctcatga gccctatgat ctctttaggt tttttgcaga taattgcaat tgagtctttc
     1801 acttccgatt ccgtgacagt gtaacgtttt atgtcgtcta ttcgcagcag accgtcgcaa
     1861 caagccataa tcaattcagt gtcaatctga ggtatgttct ttttcagctc atcgtctata
     1921 atttgattta attttctttc cagagatctc atgttttctt ccattatttc aacctcccaa
     1981 tatatatgtc gaattttttg atttgtgaca aaaattttta aaaaattttc aaaaaaaatc
     2041 agcaggcact ttttcatgtg cctgctcagc tgttttttta ttctatttct gcaccnnnnn
     2101 nnnnncatat cataggaaag tcccaggcgt tcaaaatact ggagcattaa ctcctccggc
     2161 gaaaaccttt ccggcaagct tgcatttttc gtaacagact atccaaccct caagcccgtg
     2221 aagcgcgcgt tcgtcaactc cgccctcgtc atcggcggca gttgacagga aatagatgtc
     2281 cctgaattta tagtcactcg aaaaaagcga atttgcacgg tcaagcaatg ttttcatctg
     2341 accgctcatc tcataataat aaatcggcgt tgcgaaagct ataacctcgg cgtttttcat
     2401 tttttcggta atttcaagcg catcgtcacc gattacgcaa tggccgagat tctgacaggc
     2461 aagacagccc ttgcaaaacg ctatattctt gcccatgagc gaaacggttt caacctcgtt
     2521 gcctgcactg aaagcaccgt cggcaaaagc ctttgcaagt gcctcggaat tgctgttttt
     2581 tcgcaagctt gtacttataa taagaacttt tttggacatg atttgttcct tcctttcgac
     2641 attgacatat ataattcttt gctgtataat gattatatca tctaaactta actttaggtc
     2701 aagtgttttt tgaaaaattg tgaggtgttt ttatgtacag tatcggtcag gtctcggaaa
     2761 aattcaattt gccggtttca acnnnnnnnn nnaactcttc gttattacga caaggagggt
     2821 cttctgccga acatcgagcg ttcctccggc atacgcaaat tcagtgaccg tgaccttgaa
     2881 acgctcaatg ttatcgagtg tcttaaaaag tcgggtcttg aaatcaagga tattcgtcaa
     2941 ttcattcagt ggtgtgccga aggtaacggc acatattcca agcgactgga gctttttatc
     3001 aagcaaaagg aaaacgttga aaaagaaatg aaacgtcttg aaaaaacccg tgccatgctt
     3061 cagttcaagt gctggtatta tgaacaggcg ataaaggacg ggaacgagga taatttgaaa
     3121 aaaatgcttc cggacaagct gccgcccgag atacagaaaa tatacgattt tgcacacgaa
     3181 taaaaagcgg gagatttaac atgaacaata aaccttacag actgggaatt ttggtcggac
     3241 gctttcagac tctccattcg ggacacgccg acatgataaa cagggcgatt gagctttgcg
     3301 acaaggtcgg aatcttcgtc ggctcgtcca acgaatccgg cacaagcaaa aacccatttt
     3361 catatgagac gagagaggat atcctcaaaa cggttttcgg cgaaaaaata tacgtctacc
     3421 ctctccccga tataggcgtc ggcaacaatt caacgtgggg agattacgtc ctgaaaaatg
     3481 tcattgaccg cttcggcgaa atgccggatc tgctcatatc cggcaaggag gcaagacgaa
     3541 taagctggtt tgacagtgtt gagggtgctt caatcgccga gctttatatt cctaaaacaa
     3601 ttgatatttc agcaacggaa atgagagatt ttctcataaa taataatttt gaaagctgga
     3661 aaaaatacac cgacccaaag ctttggtgca agtacgggaa tcttcgtcaa gaggtgcttt
     3721 tgtctaagga caataacaaa acgcacagta tataaaaaac gctgaggctt tacgtaaaag
     3781 tctcagcgtt attcttttaa ttatcagtca ttcttctgcc aatagccgtt gaggaagtca
     3841 ccgatatcac ggatagcctg tgcgatctcg ttcggctcgg gaagcataac aattctgaag
     3901 tgatccggct catgccagtc aaagcccgaa ccgggaacga tgaatatgtg cttctcatga
     3961 agaagatcaa aagcaaactt cttgtcattt gtaatgttga acttctttac gtcaagcttc
     4021 gggaacacat agaacgcacc gtggtttttg acaaccgaaa gtccgtcaat cttgtcgatt
     4081 tcacggataa cggcctctct ctgctcataa agtctgccgc ccggaacaag catagcctgt
     4141 gtgcttgcgt tgtcttcaag agctgcagga ataacgagct gagtaagagc gttgccgcaa
     4201 agacgcatgg cggcaatctg gaatataccc tcaacatagt cgtcggcctt gcccttatag
     4261 ccgctgatgc acatccagcc gcagcggaaa ccgcagacaa tgtgtgactt tgaaagtccg
     4321 ttcattgtga taaccattct gtcgggagcg agagcggctg tcgaatagtg ctcaacgccg
     4381 tccataacaa gtctgtcgta aatctcatct gagaaaatga gaagatcgtt ttcacgggca
     4441 acctgaataa tgtcctcaag aacctccttc gggtaaacag caccggtcgg gttgttcggg
     4501 ttgatgataa caatagcctt tgtcttggag gttaccttgc ttctcatatc gtcgatatcg
     4561 ggataccaat tgttttcctc gttacactta tagaaaacgg gctgaccgcc tgcgatataa
     4621 gttgagttcg accaaagcga atagctcggc gagggcataa gaacctcgtc gcccttatta
     4681 agaagcggaa gaagcgccat gagaacaagc tcgctgacac cgttaccgat atatatatca
     4741 tcgggagtta ttccctgaac acccttgctt ttgtgataat tgcaaattgc ctcacgggca
     4801 tcgggcatac ctttaagatc acagtatgca acagccttat cggcattgtt gaggagtgca
     4861 tttcttacgc tgtcgggcat tccgaagccg aatgtggcag ggttacctgt gttgagacga
     4921 agcaccttaa tgccttcctt attcattctc tggctttcaa gataaagcgg acctctgatg
     4981 tcggaatgaa ccggctgtaa attttctgaa cgtttgattt ccatagcaaa ctcctatccg
     5041 ccgctcaaat agtgcggcaa tttttatctc atacaatata gcacaattta atgtatattt
     5101 caagaataaa aaccaataaa aatgaataat attgaatttt aacaataaca tgcacaaaaa
     5161 gctgttgaaa ggtgtatact ttcacacagc aaataaaagc ttttacaact tgaacaaatc
     5221 ataccgtaat ttttcggtaa aaacgaacaa gaataaggtt ggctcgaaaa cattttgctt
     5281 ctattagggc aatgcttctt ttttgtctca gaccgatgac gggcattatt cctacggaat
     5341 tcgacatttt tgattccatc gtattcactt ttcccttttc tttcaaaaaa caatctcccc
     5401 tcgatcaaat caaggggaga atcggcataa ttattgttca accgaaaaca ttttattttt
     5461 ctcagaatat gaagctccag aagtcaccga agcccttttt gccgaagatg agagtcaaaa
     5521 cctttgcgac tgcaccgaga gcggaagtga gaaccaccgt tgtcttgtcc ggctccgatg
     5581 caggcttgta gtcgcctctc ataagtccgt cgtagtcgag ctgttcctta acctcttccg
     5641 ttgcaaggtc atactttgca acaagctcag ccataattga acgcatattc tctgtagtct
     5701 tgttgtcggt ctccgggtcg attaccgtgt ttgcgataac ggcgtttgcg tcggcaactg
     5761 cctttctgag tgccgcatca tcctcagccg agagaacacc aagcttgaat acctgctccg
     5821 catcaccgag ataacggttc agcttcttga ggtttctcac attattgaac tgcggataag
     5881 tatccttgca atcatcgatg ctcttcacct tgttcattgc gatatcataa gcaaggttga
     5941 gagcgatgtt attgtttgta agctcgtgat actgcttatt gaagtaccat gtcgtcttct
     6001 cgaaataagc cgttgaggtg tcgatagatc cgtcgggaga aacatggtga gacgggtctg
     6061 cgatatattc gtcgctgaat gatgtgcccg ccggaacata tgatgtgccc ggagccgttg
     6121 acgagatctc gataacctca tcggagttcg ttgtatcctg agttnnnnnn nnnngccgag
     6181 gttatagcct gcaatgaagt aaagctccgt gccgaacttc tcctgctgat actcaagtct
     6241 ttccttgact tcacgctgag cctttgcgta gctgtctgcc tgagcctttg tctctgtatg
     6301 ctcctcgccc tgaagatact tatctgcaaa ctcctcgtat ctgtcgggag ggcagagaga
     6361 aataagcgat gtgttcggta cgataagcgt cttaacaaaa gcggaaatta tgtcataaag
     6421 aaggttgtcc acttcctgct tcggaagaat tcttgctgca atattgatta gtgagccggt
     6481 catagcgtca acaccgatct gctgaatagc gtcggtataa accaaatcgg gagcgttctc
     6541 gtcaaagtct gcaagcataa ggtcggcaaa aacgctgctg ccctgccatg cgcctacgac
     6601 gctgattatc ttctcaactc tgcctgcatc gggatagaga ttgagatagt tgtttacaac
     6661 gcttgctccc atgctcatcg gaacaaggat gaccttatcc gagcctgtct gcggcagaac
     6721 aacattctct atgtaatcat taagaccctg tgcattctca aatgtgtttg agaaaatcga
     6781 atagttgtag caataaacat tgtcctcacc tatctcgtca acgagagcct gacagggaat
     6841 acgtctgtta aaaattcctc ttgcgtcctc gttgtatccc gaaatcgggc aaggatagtt
     6901 aggtgttaca acgttgttaa cgaacgagcc gtcggcattt ctgagatggt caacaaagag
     6961 aacctcaagt gctcccttga tcgtgctctc ggaaacgacg tcacgctgaa ccgcaattgt
     7021 tacaataagg cccgcaacaa gacgaatcat cttgacccaa tcaataattg agtagcttgc
     7081 aaagtccgtg ttgacatgga gcatattcca gcttgcgatc tgcttgccct cgtcatcata
     7141 tttgtatgtc tgcgactggc cgatacccgg aataaaaacg accggtgtaa catcgtcctt
     7201 tgcgttcgcc gttatggcaa cagccgacat ggtgaaaacc ataatcacgc aaagaattac
     7261 ggaaatgatc ttctttgaaa gtttcataaa ataacctccg ttatatttta tcaagataat
     7321 aataacaaat atatcgaata aaatcaatat acaattaaaa ttttatgcaa cattatgcaa
     7381 aaatcttaat tggaaatatt tcacaaaata tcgaatatct ccgttatgca gcttatcgtg
     7441 taggttgcat cgcttacggt tttttgattt ttcctgttca cccagcagga gtcaagtccg
     7501 ctgttttcgg cgcctgcaat atccgaagaa agagaatcgc cgacgacgag aa
//
LOCUS       DAWBSW010000097         7455 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1996_length_7455_cov_2.551486, whole genome shotgun sequence.
ACCESSION   DAWBSW010000097 DAWBSW010000000
VERSION     DAWBSW010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1996_length_7455_cov_2.551486"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4817..4826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2084 a         1728 c         1347 g         2286 t
ORIGIN      
        1 gatcaatccg tttaatccat cggttatctg atcatgcacg gttgaaaaat tcgtaacaac
       61 tatcggtctg caaagacatt tcgcttcgtc gattgctatt gacttaccct catacaatga
      121 cggctggaca taaatatcac actgtcccat ataaggatac ggttccgatt tttcaccgag
      181 caaaataaat atattttgca aattattttc agaaatttgt ttttccgttt ttcccttcag
      241 ctgaccgtca ccgatgtgat accatttaat gttttttcct ctttttatca gctcgccgca
      301 agcatcgacg gcaagggaaa tgtttttttg ttcaaaaaat cttcctaccg tgcaaagaac
      361 ggtttctttt ctttccggat atgcatcaaa tttcttgctt tcttcaaaaa caatttttct
      421 gcttgtaata ttttcgatca cgcagatttt atttgagaat ttctcaaaat ttttaagcag
      481 gcttttttcg cactccgccg agacagtaac aattttatct gccgcggaaa aagttttttc
      541 gcaaacttct ctgtcaaagc caagcttttc aatatcatta tgtatataac aaatcttgtg
      601 cacggctctc acattttcgc aaacaaatcg atttgcgttt ccttcaagat atcctatcgc
      661 acaatcatat tcttttttta ttttcggaag ctgttttttt agatatttcc actgaatttc
      721 ttcgacctcg tgagtgtcgg cagcagaata aattcttgaa tatttccacc tgtcaacagc
      781 agaaaaaatt tttccttttt tcagaaagaa attaactgcc cggacggcat cgccgtcaaa
      841 tatttcatag ttcttgcttc cgccgataat gttgacctct ttcgggattt tctcaaagaa
      901 aagtccgtca tgtcgaaagc acaaaaggtc aacctcgtat ttttcataat caaacagcgt
      961 caatacggtc aaaaggcttt tttctgcacc gccgcttcgc atagagggaa tgacaaaaag
     1021 tattttcttt ttcaaaatac cagctcctgt cagattttca aaagcatttc tcccgcttcc
     1081 attgcgcttc tcattctaag cggaatactt cccatcagtt ctttcctgat ttccttttcg
     1141 ttgacaacca acttcaaaaa ttccttttcc aaaacctttg agtccgtaag cgaattaaag
     1201 ttcaaaacaa atttttcttc gccgaaaaca tcctttgcaa tgcctctcgc tttaacactg
     1261 taccccaaga caattgtcgg aacaccgctc gaatatgcgg cgattgtcgc atgcgtcctc
     1321 gctccgacaa aaaaccttgt gttggctata tagcccttat attgcgtcgc gttgagcgtc
     1381 gacggaagaa tcgcaattct ttccgtttcc gcaaactttt cataaatggg tttcataaaa
     1441 tcataatcat tgttattctc ctcaaccaca tgagggacaa gcaaaatttt cagcgttgtg
     1501 ttttccaaaa caaaagaaat aaaatcctct gcaatttctc ttttttcgga tcaccctttg
     1561 caacaagggg acttatattg aagccgagcg tgttctctct tgtgaagcct tcgatcggtt
     1621 cgacattttc tcgctccatg caaaaggcag gatcggaaaa caaataaata ttttcgtttg
     1681 catttttttc tttcagcagt tcataggtta tcggttctct tgcaaatatc gcatcaaaat
     1741 caccgaggct ctccagcttt tcgtcggtaa gatcggtctc gccgattgac gctccccaga
     1801 gaacggtctt tttgcctgtt tttttcagct ctcttgtaag aacctggatc tcgtgattat
     1861 cgccataaca gtatgtgtcg ccgcctatgg acaaacagat gtcgcaatcg gcagcatctg
     1921 aaactatcgg cgaatacatt ttttcgagtg catatatttc cgacttgttt attttcaaat
     1981 caagcgttgc aattatcctt tcgggcaatg acaactctct tctttttgcc ggttttattt
     2041 caatatcatc aatcacttca tcagcttgag aatcataaga ggatagaata aattctgcat
     2101 ttttatcact gttttcaatt atattcactg ttcccctgac aagtgcttcg cagccgtgat
     2161 ttttgctgcc tgcatgatta aacaggaaat atttcattgc aaacacctca tctgaaaagt
     2221 tttcttcccg gtaagaaata atccgactga tacatattta tctgatatgc cataaatcgt
     2281 gaaaacagga agaacagaat aaccgttccg gttgtgtaaa ttgccctgtc ttgcggttta
     2341 acggcatagg ttactaccca accggtgagt ataatataat atacactcaa ataaattgcc
     2401 agtcttgcaa aaatccagtt gtagcttgcg acaatatata ttgcagcgtt caggaaagcc
     2461 atatttatta aaatgtcccc gatatggccg agcctgttca tacggcctct gttcaggtag
     2521 gcaacaacaa tcggcgcggc tgcaacaatt gccctgaaca gactgctgcc gccttctctt
     2581 ccgtctgtaa accatccgtt attcgcatat ccctcatagg acgtttcttt aagcgcgttc
     2641 aggaaagacg gaagaattgc gtcaaaagca acagtaacaa caatgcttcc gagcacaata
     2701 atatatgaac tcattttcca tgcctttcgt cgaacgacaa agaatactac cagcattatt
     2761 aaggcactgt tgtgcataca tgccgccaac aggacgaaga ttgcatattt aaaaaacgag
     2821 cctcgtttta acgagaacaa aaatcttgtt ccgaacaaca gtatcgctgc tgccatatac
     2881 tgtctcattc cgtccatcat tcctccgaga tatccgtaag caaaaaacag aaatatcgaa
     2941 agttcaaacg gggcagaata tatatacaat attatcagcg gaggaaccaa agcaattaca
     3001 gcgcatacaa agatcaatac ctgcggatca tcggtaaaat ttcttatcag gttttgaata
     3061 aaagaataca tttgaccgtc gagaatcatc tgaatgttaa ctgtattttt atcaggcaaa
     3121 ttctcgaaag aatacatata aaaaaatgtg tctccgatag tttttctcaa gcctgcaaat
     3181 gatacaaaaa aagctatcgg aagaaggacc gcaatgggat tcggcaacgg ctgtccgagg
     3241 cctcccctta ctttaagcgg aagagaacga gccgccaccg ctatcaagca cataacaatt
     3301 gtggcaaccc agacaactat ttcatagaaa tcgaaattaa agtttatcat tccgttcctt
     3361 tcctgtaaaa aagactcaaa aaaattcatt ataaaccact tcggcctctt ccgttacctt
     3421 ttcttttgaa aaaggccgaa cagcctcgat cccattgtcc gaaagccggt tataaagatt
     3481 tttgtcgttt ataatcaaag caattttatc ggccatcgct tcatcgtcat tcggtgcaac
     3541 aataaatccg ttatatccat cacggcaaag ctcgcggtgg cctctgttat ctgccagcac
     3601 aacgggcaaa ccgcaagcca tcgcctccat cacattgact ggcaatccct cgcggagact
     3661 tgacgcaacg gcaatgtcac tcagatgaac aaggtcgcac gcgtcgttcc tgtgtccgag
     3721 aaaagatatg cagtcagaca cgccgagttc ctcggcaagc ttaatgtact tcccgttata
     3781 attatcctca ccgacgatga gcagtctgat tttttctccg cgccgtttca gcctttgtac
     3841 cgtgtgaatc agcattccct gatttttgtt ggcattcatt tcggcaacat aaatcaaaac
     3901 agtttcgtcc tcggcaactc ctttttcctt gcgaagccga agtttttcgt catcggaaat
     3961 tttatagaaa aggtcgctgt tatagccgac cccgttgatt ttaacggttt tcttcgcttt
     4021 caggtgtttt cttgcaaaaa aataatcttc tttgtttatt gtaataaggc aatctgtaat
     4081 tcccgacatc acaagctcga tgggataata aatcaaccag ttgagcaagg gtgcgccctt
     4141 gtaaaaatga aacccgtggg cagtgtaaat tacttttgtt cctttttttc gctcaccttt
     4201 tgccgcaagt cttgcaagga ttcctcccat cggagtgtgg caatgaataa tatcataccc
     4261 gcccgtttta attattctct tcaactcttt atatgctttg atattttcct tatcgacggg
     4321 ggatctcttt atgggaattt caaatttatt gtcgcagtac ggaagttctc tgtcaccgtg
     4381 gcaggcaacg tcaacctgcc atcctttgtc cttgaacatt ttgaagcaag gcagatggaa
     4441 ataaaaaaaa tgctccgcaa cggttgcgac aaaaagaatt tttttcattg cgaaacatcc
     4501 tctcactcgg taaacttact aaagcccgtc ccaaaagttt atatgcatcc aaatcaaact
     4561 ttttcggact ctttattccc tttgaatttt tccatagtat tttcttttcc gctgttgatt
     4621 ccgtcctggc ggataacatt ttcaatcgtt ttaaagaaaa ttttcatatc catgccaaag
     4681 ccgtaatggt caacataata gacgtcgtaa ctgaattttt cgttccatgt gagcgtattg
     4741 cgcccgttga cctgagccca tcccgaaatt ccgggcagga cctcatggcg gcgcatctgc
     4801 tcgggagaat aaaggtnnnn nnnnnngata ttccgtaagc atcggtctgg ggccgataaa
     4861 gctcatgtcg cctttcagaa tattgaaaag ctgcggaagc tcatccacgc ttgtttttct
     4921 gagaaatctg ccgattttcg tcattctctc aaagtcactc agctcacgtc cgtttttgtc
     4981 atacagcctt gtagacatgg ttctgaactt ataaagcata aatattttac cgttttttcc
     5041 gggtctctcc tgtctgaaaa gcaccggtcc gtcacggtta gccgcaatgc aaaccgcagc
     5101 ggcaagcatt aacggcgaga ggatgataag cagcaaaaaa gctacaacaa aatccattgc
     5161 tctttttata tatttataca ccggattcac tctctcgcaa aaattatttg tcagttgttc
     5221 tttgcttcat ggtagttggg aacaatttcc ttaataattc cccttacttc atttgcatct
     5281 gcatctttaa gcttttcaag ctctttttca aagagttcgt catccatatt aacgggcttg
     5341 gtgacaaata ttttattgtt tgcggtcatc tttctctcat cgctttcttc gtcaagaaca
     5401 agctcctcat aaagcttttc gcccggacga agacccgaga attttatttc aatttccgta
     5461 tagggtttat aacccgaaag cataataagg ttctcggcaa gagttacgat cttgacgggc
     5521 tcacccatat caaggacgaa tatctctcct ccctttgcca agccgcccgc ctgaacaacg
     5581 agctgagccg cctccggaat ggtcatgaaa tatcttgccg agaacattgc cgaaacgaac
     5641 agccgcaaag atggtgctgt ttttgcattt gcgctcaaaa tactgcacta tcatttccgt
     5701 gattcttttt gttgcgccca taacgtttgc cggatttaca gccttgtccg aagaaatatt
     5761 tacaaaacgt ttaacgttat attcgacagc aatgcttgca acattgaatg tgccgaaaac
     5821 attgtttttt accgcttcct tcgggctgtc ctccataaga gggacatgct tatgagccgc
     5881 cgcatgaaaa actatcgacg ggtggaattc gtcaaaaacc tctctgagtc tcttagggtc
     5941 tctgacggaa ccgattctta tgtcgatttc gggattgccc ttgtggtaag catcaagctg
     6001 ctgtttaagc aagtaagcgt tgttttcata tatatcaaat acaacaattt ttttcgggtg
     6061 gtactttgaa acctgacggc aaagctcact gcctatggag ccgccgccgc ccgttacaag
     6121 aacgatctga tccttaagat atccgcaaac gtcacggttc agctcaactt cgggacggga
     6181 aaggaggtcg gttatattaa catcgcgtat tctgcctcca tgagaggtgt ccgtcacttc
     6241 taaaaggctg ggtgaggtct tcagcttaca accggttttg accgcaatac gcaaaatttc
     6301 cgtttgtctt tcgctaccga cagacgggag acacattata atcgtattta tatcatattt
     6361 ttttgccagc tcgggtattt ggtcgcatgt tcccttaacg ggaattcccc tgattctttt
     6421 gccgaccttg agaggattgt catcaacggc aataatcggc tttccttttg aatagttgtt
     6481 gacttcaagt tcattgataa tgatcatgcc catgtcacct gcgccgacta tcataacgcg
     6541 gtcaagcttt tcgtcctttt caaggctctt ctctcggtag taccttctga caagacggta
     6601 aaggattctc attccggcaa caagaattat tgctacaagc gttccgaaaa catagaaata
     6661 tgcatcaaaa cagccgttgt taagctttga tgccgctccg ccgatgcttg aaagtatcaa
     6721 atcgaccgca attccggaaa ttccaccgat tatagtcgcc aaggcaacat tccagacttc
     6781 cctgattccg gcatattccc acatgcttga gtaaatctta aaggcataat acaacacaat
     6841 aaaaattgcc gtaaccggca gtgctctctg ccatatcgcg tccatattat gctgaccgct
     6901 gccgataaac ataacaaata tgtaagcgaa atttaccgca aaaacatcgt acagcatcat
     6961 caaagcactt ttgattcctg cgcttttgaa atcaaccttt ttcacaacaa ttgacctccc
     7021 aattaaaaaa tccttttctt gatctttcga ttctgcccct aatatacact gttctatttt
     7081 aatatattat ttcactaaag tcaagcaaac gttctgtata tttgttttat ttggcaaaat
     7141 attatctatt tatagtaaat agataacaga atcatatctg ttttttcctt tttatgatca
     7201 cataagcttt attttcggtt gacagccctg ttgaaatatg atacgctaaa gaaaaaatga
     7261 acggagcgca aaaaatgata ggaatcggca aatgggaagc ctcaatcaat acaatgcttt
     7321 tcaaaggaac aggcagagtt acaataagcg acaacaacgg gaaatatgat ttcaagcttg
     7381 aaatagtcgg cgaaaatgtt cccgaattca ttgtcacgga tgttattgag gaaggaaaca
     7441 ccttgcgtgc cgttg
//
LOCUS       DAWBSW010000098         7445 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1998_length_7445_cov_2.963194, whole genome shotgun sequence.
ACCESSION   DAWBSW010000098 DAWBSW010000000
VERSION     DAWBSW010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_7445_cov_2.963194"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4320..4329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1975 a         1721 c         1447 g         2292 t
ORIGIN      
        1 caaggagcca gtcgacgata gtaaactttc ctgcccaggc tttaaagatt tttatgtact
       61 gcggaatatt gaaaatattg gcaataccca agccgatacc tttaaatata ccccaaacac
      121 cttttacgaa aaactggttt aaaaattctg caaggaactt tgtgattgtc atttcggtca
      181 ccctctgttt ttaagtttta tattggtatg agttgaatgt tatttttctt ctttattttg
      241 ggagttttct ttcttttgct gagccagata ttcatcaatt accctctgct ttgactcttc
      301 attaaggctt tctgccttgg cagtcataaa catggaaatc agcttataaa tctgccaaat
      361 aaagaacagg gcgagcggaa ttatgatgca aatcataaag ccagttctgc tttgaaggaa
      421 actgagcgct gaaccgaaat tagcaatctt cattcccgtg agcttttttc ccgtttcttt
      481 gtctataccc gagttaacct gcttggcaat aacctcgtca ggaagaacgt ttacggtatc
      541 ctccattcct acggcatcac cggctgtaac aaacgcataa accgagccgt ccccggcctt
      601 cagtatatcg gtaattctat gcgtattata gcctctgatt tttttgcctg ccttttcacc
      661 ctcaataata gtggtgtaag taataacatc gcccacctga agtgtttcgg gatctttaac
      721 ctccttggag attatcagat cgccggttcc gaagcccgac ggttcgccgg aatccattga
      781 atcagactgt acggcaaacg gcgtatatcc gagaacattt ccaatgccca tatccgaatt
      841 ttttgaggta aacgtcataa ccgttataat aatcgcaatt ataattataa taatcagcaa
      901 tatgtcggaa ataacacccc aaatgctttt ccctttttca ttcatttaca acattcactc
      961 ctgtattttt ttcctaaaaa ggtcatttgt catcacagcc gtctgtgttg acaaatcgcc
     1021 cttttagctt aggatcacgg gtgacaccgg gttagcggtg tcaccctccc aaagtgacgg
     1081 gttaaactcg aatcaacgat ccgaatgttc cgaaaatata caaattagtc tatttcaaga
     1141 acaagattga agttaagagt tgcgcctgca acatcgtttg cacagtcaac atcattacct
     1201 tccatccaca tgtaaactct tacacgcgtg atacctgctg caacagtaac tcttgcctgt
     1261 gaggaagatg ttgcgagtgt agcctcgagc gggcttatgt aagttacatt tgtaacaatg
     1321 ttgttgtatg tcgcgtttgt gccgatatta agacctgctg ctttgagcgg gcttgttgtc
     1381 atgattccgc ctgcaccgag agctgaagca gctgcagtgt ggttagcggc gtcaacttca
     1441 taaacaacct tcttggtttt gtctgtaccc ggaagaacgc tctggatagc agcagcgtct
     1501 gccttttcag ctactgtgcc gcagtttaca agtgctacac gcatagctgt gggaagctca
     1561 gggttgcctt cgcatgttat cttcgttgaa ccgaacttaa caactgtttc tgccttaacc
     1621 tttacgaaaa ggtcaaatgc aacaaatccg ctgtcgacat cggatcttgc gtagatgtcc
     1681 attctgcctg aatcattgat tgatccgtcg aagaatttcg gaaggtttcc gttggttgca
     1741 aaaactgacg atgacggctt aagagtaccg ggaatattgt tcatatttcc ttcatatgcc
     1801 gagaaacgtg ttgttgtggt gccggaaatc tcatcagctg taagctttgt tgtgaaagca
     1861 gaggtatttg cagaaatctg aataccttca ggtgatgtga cgttcaattc catggactcg
     1921 acttcaactc tttttgccat tgagaaccat gcatatgtag ctgacgtaag aacgatagca
     1981 gatacgataa gcattgcaat ggcagaaatg aatgcgcgct ttttgaatgt ctttttcatt
     2041 atctttacct ccataaagat ttcatgcctt ggcgaccgtt aacgcgccgc caaacgcata
     2101 atttttatcc gaattgtctt tgatatccgg agctttgcat tagttcgatg aaattaatct
     2161 tatatgtgta ggcttcccgt cacgtaagct tatacataat cataatcaaa tttcatcgag
     2221 agcttaatgc ttccgcccaa aatgtcattg acacaatcag gatcccagcc ttcaagccac
     2281 attactatcg tatatttatg atagctgccg gcttttgtaa ggacgttctt atcctgcatg
     2341 actgttgaat catttataaa cagtttatcg tccttattat atttatcatt ggtgtcaaat
     2401 acgtcggtcg ttcccttttt cggcttagcg taaatagtgg gtgttccgcc gtctttgatt
     2461 accataactc tgaccgcctc atcggcaccg agatttacag attcaatctg aaggtttgcg
     2521 gtgtagctga catcaacatc tcccacattc ttagcataga atgtgtatgc aaagaattcc
     2581 ttgccgtttg aacggttatg agaaccgtca atactgtcaa gctctgccgc cgacggaagc
     2641 catgtgtatg tgcagtcgcc catatcgttt acacttgcgc cgctgagcac tgccttcata
     2701 tcggagaaat ctccggattc ggaaaggaga agtcctcttg tgggctccga cttattgatt
     2761 atcgctgtaa agtcacctgt atcggttacg cttgtcttgt tttcaacgat ctcacggata
     2821 ctcgttactt cgctgatgct cgtcacggga acaatcgttg taacctcgat ctccttaatg
     2881 atctccttga taacttcaac ttccttgata acctctttga caacttcttt aattacctcg
     2941 acctcgcgaa tgcttgtaac ctcgctgata ctggttacaa ccgaatagct cggaacctca
     3001 acataacgtg taacatcgct gtatcttgta atgtcactgt aacgtgtaac atcactgtac
     3061 cttgttatat cgctgtaacg ggttatgtcg ctgtagcggg taacatcatt gtatcttgtt
     3121 acatcgttat aacgtgttat gtcactatat cttgttatgt cactgtatct tgtaacgtcg
     3181 ctgtaacgag tcacatcatt gtatcttgtt acatcgttat agcgtgtaac gtcgctgtat
     3241 cttgttacat cgctgaaacg tgtaacgtcg ctgtatcttg taatgtcgct gaaacgggtg
     3301 atatcgctgt atcttgtgat ttcaacaggt ctgacgcttg taacttcttt ttcaaccgtc
     3361 tttataactt cttcgattac ctcgacgcgc ttctcgataa cccttgtaac ttccttaact
     3421 cgctcaagcg tctgaattct gacgcttgtt acctctcgaa tactggtaac ctcttttgcg
     3481 gtagttgttg ctccgccgat atcatcaata tccgcatcaa ggatcttgtt gatatcgtcc
     3541 ctgatctgag taagctttgc cttgatgaag ctgatctgtc cgggagtaag ctcaagcgta
     3601 ttgattgccg tgctgactgc ctgggtctct ctgtccattt tatcaacaac tctttttaaa
     3661 tcatcaaagc tgttgatctc cggaacttca tcaagcatgg cattcttgat tatgtcgtca
     3721 tattctcttc tctcgtttat gtatgaggag catccgaaca taacagaaag aaaaatcaaa
     3781 aggagaacaa gaattatgag aagaatacga ataactgctt ctcttttctt gacctttttg
     3841 gactctcttc tgactccgta tcggctcatg cggtcctgct cgggagttgc tccgtcgcgg
     3901 ggaagcggat ttttcttatc tttctttgcc aaagccgatc actccttagt tgatttgttt
     3961 ttctttcatc gcctcgccga aataagatcc ggtgaactga tccgctccca tcttgttgag
     4021 aagatcaaac atttccttgg actctattcc gtcgaccctg acctgcgtgt ctatcttttt
     4081 gcagaaatca atgattccct gcaccgtgtt ctgaaccctt tcgcttgtca ttatcccgtc
     4141 aagcaaactt ttgtgaagcc cgatataatc aacgtcgtaa tgagcaagat gagtaagaga
     4201 tgtatattca agaccgaaat cgttaatgga aaccttgagg cccatctctc tgaacgccat
     4261 gatattttcc attacctgat ccttaaccgc ttcaagaatg ctttcattta tatcaaagcn
     4321 nnnnnnnnna atctgaacga ttatagagtt caggtcggcc tcacgttcct cgcagcgctt
     4381 aatcgcttcg caggcttctt caacctccca aagattaagc tgaacgatct ggtttgactt
     4441 ttccgctatc gggacaaaaa gcttcggtag catgactccc ataaccttat cattgataag
     4501 cattgagatt tcataatcta tcgccatatt caagtgaatg tcaaagaccg gtctgtacct
     4561 aagctcaagc gttctgagcg ttctgccgga tccggttttc tttttgtttt cagccatatg
     4621 cgacactctc catcaatgta aaatccgctg atactttttg ccttgtggcc aacaatcgac
     4681 aaaatacaac atcttgtgtt tttgtacaaa agggggcact tgtcccattg gcgcactatt
     4741 acatatatat tactttaaca tattccaaac gattagtcaa tgattttttt atcaaaaagt
     4801 gcaaaaatag ttatatgaaa tttgttcgat tgttaatcat ttgttaaatt atattacaat
     4861 gaattataac tgtattcatt gctctcagaa cagataaaat ttcagcattt atttttttca
     4921 aatcactgtt gctgccgcaa atataatcat aaaaatcctt tgcttcctcg ctcatcaata
     4981 catgcttttc cacttcgccg acaagctttt ctttcgcgcc gtcactataa cagatttcca
     5041 cgtcggtcag cttggaaatc gagccgaaag tcatcgtgcc tttgtcgccg attatttgag
     5101 agcccagata atcctgaccg atttttgaat aggtcaacgt aacttgttta tcggcatagt
     5161 caagaattgc cgtgccgtag ccgtccgctc cggttgaaag gaatcctgcc gaagaaataa
     5221 ttttttccgg ctcgccgaaa aaatcaatcg ccggataaaa gcagtaaatt ccgagatcac
     5281 gcagacagcc tgttttcatt tcagggttga aaatgttcgg gttttctccg cgaagcaatg
     5341 cgggatattt cgaagaaagc tgagagaaat caaaatgagc cgttgaaatt tttccgagtg
     5401 attcaagctt cttttttata atttttcttt gcgaaagatg cagcatcatt attgcttccg
     5461 tgtaaactaa attttttctc tcggcaagct cacataattc tgtaaattct tccggcgtta
     5521 ttgtaatcgg cttttcgcaa agcacatgct ttccgttttc aagcatcagt ttgctctggg
     5581 aataatgaag tgaattcgga cttgcaatat agacggcatc aacattgctt ttcgcaaatt
     5641 cattcaagtc cgtataaacc ggagctccgc cgaatttttc ggcaaacgct tttccttttt
     5701 catatgtgcg ggaatacatc gccgagaata cgaacccgtc aacaagcttc gctcctttaa
     5761 caaactcctc ggcaatccag cccgagccta taactccgaa ttttatcatt aaaaaatctc
     5821 ctttcaaatc atatataact ttacacattt gtatacgccg gtctcaagaa aacgaactga
     5881 cgcatttaga tgcagaaagc gctaaatgcg tcagcccttt gggaattatt ttgatgaatt
     5941 tccgatgctg acaacattga cgattccgat aaatccgaag gtcacaagaa tttgcatcag
     6001 cagaacaaaa atctgcggca aatcacatat gacattcatc gccgaaagta tcagcacaat
     6061 gacaaagccc atcagaattc cgagcattgt cagcagaccc gaaaccttga acatttgcct
     6121 cagcgttgaa gcgaggcaaa ggcttttcat aaaagtgtac ggcgtgccgt catggattgc
     6181 aacggctctt gccgaaagct tgggtcttga tttatatttt ttgaaaattc tgcccgatga
     6241 tgagttaaga attttaatgt tgttcaatct catgtcaaag cagcttgaaa tcatcgcctc
     6301 attgacgttc ggatcacagt tgcggacaag aatacttact ccgctgtccc gaagctttaa
     6361 aagatatggg cgaatctgtt cattcggagc atagtcgaca accatcatcg ccgcaagctt
     6421 atgtgctatg ccgagataca aaacctcgtg acctgctata agatacttat cctcgtcaac
     6481 cgtttcggga atctgtatgt tatggttcac catcatcaaa cggtttccga gaacgacctt
     6541 ctgaccgaag atccagcccg aaataccttg cttttcttca tagctgaagc tcttgacggc
     6601 agggagaaga tcacgtctgt ttacaaccac ctgatcgaac actcccataa gcggtccgcc
     6661 cgagcggata accattgctg cggcataaag catcgcatcg tcaaggcgta catttttaaa
     6721 atccttgaag ccgtgcatcg agcaggcctt ttggtcaaag agatctttac tgtcgataat
     6781 aacaacattc gttctgcaaa gctcgttaac ggcatcctga caggaaatca ttcctccctc
     6841 cttgttgaac tttttgtttg cacggctgag cggcagctcg taaagcagcg gggcataagc
     6901 aggaacacac atagcaaacg ttattgagaa tgcggcaaag cccgtcgcaa aatctctttt
     6961 gaccgcccat gcaattgcaa gatttatcag cgccagaagc gcggtaagcg gaagcaaaaa
     7021 tcttgtcatt ttatctgccg aggtttcgct catacaaagc tcaatgaggt gcgccgggaa
     7081 tttggtcttg caggaaaaac gaattctcgg gctcgtgttc ttaacgttct ttgcaagctc
     7141 aaccgcatct ttatcgtcgg aaattccttc gatactgtac agcttgtcct tattgtcacc
     7201 cgtgcacatt tccattgcgt caaccgttcg cgaatgaaca atataattgt ttatctcacc
     7261 cgtcaagaga agcaccagcc ctgcgcccga aaaaacgctg acagtatttt tgtcggcaaa
     7321 ataaaaaatc agcatcaaca aactttgaaa gaaacaaatt atcgccgata tggaaatcat
     7381 cgtgttaata tttatttttt ttcgcctgag catttcaaaa ctgccgacta tagattgatt
     7441 tgcaa
//
LOCUS       DAWBSW010000099         7445 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_1999_length_7445_cov_2.494046, whole genome shotgun sequence.
ACCESSION   DAWBSW010000099 DAWBSW010000000
VERSION     DAWBSW010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_7445_cov_2.494046"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2068 a         1549 c         1760 g         2068 t
ORIGIN      
        1 aaacagcata acaacacccc cgaatctcaa aaaggttcgg gggttgtgct ttttgtcaag
       61 aaaaaagcag ccgcacagtt aagtgcagcc gctctgaaac tgattcgata ttttttgaat
      121 ttgtgctacg gtaacgagat accttttgct gtaaaacaaa cgtgttatct tgcttcgctc
      181 atataaacgt ccattaactt gcccgaccat tcttcatatg tagcgtattg tccgtcggta
      241 ccttttgccg tccacatata ttctgccatt ttgccaacgc cctcgtttgc gatttctgcc
      301 agctccgaaa tacttgaagt cggtgctgcg tcccggatct tttgggcgta ctcattatat
      361 attgtttcat attttgaaac gccttgagtg gagtgcgctt ttgttttgtc ggtactgctt
      421 ttttcagact ttgattcggt cggttttggt tcagcctgtt ttgtagtatc ttttgaagct
      481 gacggtgctg tgctgtcctc gtcaggagcg tcaatatcaa tgctcataat attgttgccc
      541 tcatatttga tcgaaacgtg ccagcccgca ctgttatcgg cattataaaa gctgtcccct
      601 ttgtcataat taaccgtaaa acccttatcg gagcaagctt ttacatagct gttgtaatca
      661 tctttgctcg tgtttccgat gtaaccgttg aagccgtcat cgttttcatg ggtaaactta
      721 ccttttgtgg attttggagc ggggagctgc ttgcctgctt tgcttgttgg ccatgtgatc
      781 gaggacatct caatcggttt cgccaattcg atcttcattt cgtcttcact gccgtaatga
      841 ctcaggctca atttgtatcc ctcggaattg tatgcgccgt atgacatgga gtccgattcg
      901 ggatcaaccg taaatccttt tgctttgcag gcttccgtat aatcgttata ctgcttgtca
      961 gagattttgt ttatatcaac ccaaagtttt tcggcagtgt tttcgtgaat ttcacctttg
     1021 tttttaggcg gttcgggtaa ttgtgagcct aaaattatat cgctccagtc gattctttct
     1081 gatttttcgc cgctcgaaaa tttgccgata atagcaatca aggctatggc aatgattaca
     1141 taaaaccacc attttttgta aatcggtttt ttctttttct tggtcggcgt aggctcggta
     1201 gggtatgtac cgggggttgg tgctgcaacg tttggtgccg cagtgctcgg agcaggcgct
     1261 ccgcagtgct cacaaaattt accgtggaac tcttttccgc atcttgaaca aatcacttta
     1321 atgtctcctt attatttaat agttttaatg atttttgcgt taattcaatt ttattatctc
     1381 ttaacctcct ttataaaatt ggcaagagca aatacagctt tttcggatag ttcgggcagc
     1441 gtgttttcgt cggagcgccg gacaccgagc aggtaatccg tgctgacatt aagcaacatc
     1501 gctatgtcaa ctatgtgctg caaatttgga acaatatctc cactttccca tcgtgaaacg
     1561 gccgttcttg aaacatcgac tgcctccgcg agattttcct gagtgaatcc tgcaatctca
     1621 cgagcaagcc gaattcgttt tccgatatcc gttacaacca tttttttacc tttccaaaag
     1681 ccttgtcttt taaaattcat aaatcatcct cctttgtgtt ttgattcact caaaagagga
     1741 cttgtaattt tcgcgaatat agcattatta tatatttttt attttggtat aaacagcaac
     1801 tgacggtaaa atttgtaaac cgtgagttac gcgggaactt tttacttgaa aatatcggat
     1861 cataatgata taataataga aattctaata ttagacaatt tggaggacgc tatgactgcg
     1921 ataaaaatga tagctgttgg gaagaagctg gcgaatattt acagcgagct ttcacagcct
     1981 ttgtgcaaaa agtacggaat aaatcaatca tgctttgacg ttctgatgtt ctgcgcgaac
     2041 aaccccgaga ataatacggc gagggacatt tgcgctgtca ggagaatcaa aagcggaatt
     2101 gcttccgttg cggtagaaac gcttattcaa aaaggatatt taatccgtac ggacgatatc
     2161 tcggacaggc gaatccgccg tcttgtgccg actgaatttg cacaaaatat aatttccgac
     2221 ggcagggtaa tgcaggaaag atttatttct acgctgaaaa gcggaatttc ccctgaagag
     2281 gaaatctttg aaagagtgaa tgaaaaaata cttgagaaca caaatttgtt cgatcgggag
     2341 gaagcataat gccttttaaa attataatct gtattattgc gggtatcggg gcaggcttgg
     2401 gcaccggctt tgccggaatg agtgccgccg cagtaataag ccccatgctt atcacatttc
     2461 ttgatatgga ctcttacctt gccgtcggaa ttgccttggc gagcgatgtt cttgcaagtg
     2521 ccgtttcggc ttatacttac ggtaaaaata aaaaccttga tatccgcaac ggtcttatca
     2581 tgatgttttc ggtgctctgt tttacgcttg tcggcatcta cgttgcgagc attgtgccga
     2641 aaacgaccat gggtagtttt tcggtgttta tgacccttct gctcggcatt aaattcatcg
     2701 ttaaacccgt tattgcaccg aaagcgagat tgcaaaatgc cactgctaag caaaaggcga
     2761 ttcgctcggt gatttgcggc tctatggttg gatttatctg cggttttatc ggtgcaggcg
     2821 gcggaatgat gatgcttctt gttctcacaa cagtgctcgg ctatgagctt aaaacagccg
     2881 tcggcacgag cgtattcatt atggcggcaa cagctctgac aggctctgtc agccactttg
     2941 cgataggcgg tgcgcccgat cccgtggcac ttattacatg cgtaagcagc acccttgtct
     3001 ttgctctcat tgcggcgctg atcgccaaca aggtttcggc aaaaacgctg aaccgtgcaa
     3061 caggtgttgt tctcgttgtg ctcggagcgg caataattat cgtaaacaga ataaaataaa
     3121 gcagaaacag cgccgttacc tttgacacgg cgctgtttac gttgtataat atgtattatt
     3181 attactgaat gggggcaatg atgtgtccga ttataaatat gaatttctcg gctcgggcat
     3241 atatgttgcc gtatcaaagg agcatacctt cggcacggat gctgttctgc ttgccgattt
     3301 tgcttcaccg aagcgcaatg aaaaggcgtg cgatctcggc acgggctgcg gaataattcc
     3361 tatgctctgg tgcagaaata actgcgccaa agaaataacc gcagttgaat tgcaggagag
     3421 gggatattta cagacctgcg aatctgttca caaaagctcg gtcgaagaaa aggtcacgcc
     3481 gataaaacat gatctcaagg acataaaatc ggttctttct catgcttcaa tggatgttgt
     3541 tacaatgaac cctccgtata aggcggcgga cagcggcata aaaagcgttg cacctgccga
     3601 gcttatagca aggcatgagg tagaatgtaa gcttgacgat atctgttctg cagcggcatt
     3661 cctgctgaat ttcggcggca gactttgtat gtgcaaccgc ccggagagac tcagcgacac
     3721 aatttttgca atgaaaaaca acggaattga gcctaagcgg cttcgccttg tcacaaaaac
     3781 gccgtacacc cgtccctggc tgtttcttat tgagggcagg aagggaggca agccgttcat
     3841 gaatgttgat ccgcttttga taatgtacgg tgaggacggc gagctttcaa aggaagtaac
     3901 cgatatctac ggtgaatata aggagaacgt taaatgagta agctatatgt tgtcggaact
     3961 ccgataggca atctcggtga tttttcgccg cgtgcggttg aaacacttga aaaggttgac
     4021 tttatcgccg ccgaagatac aagagtgaca cttaaactgc tcaatcattt cggcataaaa
     4081 aagagcatga taagttattt tgaacacaat aaaaaggaac acggcgagct tatcgttcag
     4141 cgaatccttg cgggtgaaaa cggtgcaatt gtgaccgacg caggtatgcc tgctatttca
     4201 gatccgggtg aggatcttgt acgtctctgc catgacaggg gcgtggaggt tgaatccgtc
     4261 cccggtccgt gtgctttttc gactgcactt gcgctttccg gctttccgtc cggacgcttc
     4321 acctttgagg gattcctcag cgtgaacaag ccgagccgcc gtgagcatct tctctcactc
     4381 gagaaggaaa caagaaccat gcttttttat gaagcgccgc acaagctggg cgcaacgctc
     4441 cgtgacatga acaaaatctt cggaaaccgt gaaatcgcaa tagtccgtga aatcacgaaa
     4501 attcatgagg aggttatccg aacaacgctt gacgaagccg ttgagaagta tgccgacggc
     4561 agcatcaaag gagaaatagt tcttgtcatc aagggcgcag aatccgagaa agccgaagat
     4621 aaaatgactc tcgatcaggc tatagagtgc gcccgtcaca gcgttgaaag cggcatgagt
     4681 gtcagcgaag ctgcgaaaca ggcggcaaag ctgtcggggt ataaaaaagg cgacatctac
     4741 aaagcactga tttagcggat ctttttccgt tatacagtcc aaagccctct cggcatataa
     4801 gaatatagcc gtcgagaact ttgaactgtc taacgaaaaa atctctcgct aaatagattg
     4861 tatagctccc aagctgcaaa atgcactgtg aacaatttta attcaatcta aaaacaggtt
     4921 tcgctataat gcagctcgca aggagtttga actgtctaac gaaaaaatct ctcgctaata
     4981 gattgtacag ctcccaagct gcaaaatgca ctgtgaacaa ttttaattca atctaaagac
     5041 aggtttcgct ataatgtaac ttcaaagtgt tttgactgta taaaatattt caaagccggt
     5101 aatttttgca tagcaaagtt tataaaaaac tacttaaaaa tataaaaata taatagaaag
     5161 aaggatttta aaaatgagta tgttccaata cattgcgcag cacccgtgga taggagctgt
     5221 acttgtgctt ttgattgccc tgacaatttt tgtttggtac aaggccattg tttcgggcaa
     5281 aaagagaaac gaagaaagag aacgcattat cgccgacctt gaaagagaaa aggcactgag
     5341 aaacgagttc aggaatcccg acgaaacaac atttcttccc gaaaaggacg attacaggtt
     5401 gattgtaggt atgtgtgcca atattcaaat gaagcttgaa aaggcgtcga acatgactga
     5461 ggctttcatg gagctttccg acgttaaaaa gaatgtttac tgcctcggct atgtttttga
     5521 ggacagcaag aacaagctct cggaattctt tcgttcaaac ggtgaaccgc ttctttccgc
     5581 ttcaagggta gcggtcaatg aggcgatcgg cggtgaattc tcggaaatat tcaataagga
     5641 attcataatg cttgacgata acgacgagac tacgtcggtt gataacgagc ttttggcaaa
     5701 gtatgacgca gagtttaaaa gccttatgga cgctaagaaa aatgaaatct gcaagtcggc
     5761 ggcggattat atccgtgaga acaaggaaga atttttgaaa aaaatctgaa aaaatttcta
     5821 attacctatt gacaaagtag gtaaatgatg ttatatcacc aacataaaga aaacgacgta
     5881 agaaacggac gaattggcaa tgagaaagtc atttaacgct tgttcaatgt gtaatgagtg
     5941 ctgtatgtgc gctcttgttg tcatgccgtc ggttaagttt tgattaaact tattcagtcg
     6001 aaacaccgga aggctcccag gcttttcggt tattttttta ggaagtgata ttgtgagatt
     6061 tatttacggc aaaatgctta gaaataacat cagattcgga cgatgtatga gctgatattt
     6121 tcttttatct aaaacaagtt tacggtattt aatatttagt tttaaggaga taattattat
     6181 gtcaaagatt tatacatcag cagatcagct tatcggaaaa accccacttc tcgaactcac
     6241 acatattgaa aagaaatacg gactcaaggc aaagattctt gcaaagcttg agtattttaa
     6301 cccggcagga tcggtcaagg acagaatcgc aaagaagatg attgacgacg ctgaggctgc
     6361 gggcaagctt aaggaaggct cggtaatcat tgaaccgacc agtggcaaca cgggtatcgg
     6421 tcttgcggct gttgcggcgg caagaggata cagaattatc attgtaatgc ccgaaacgat
     6481 gtcagaggaa cgccgcagac ttatcaaggc atacggcgca gagcttgttc tgacagaggg
     6541 cgcaaagggc atgaaaggcg caatcgctaa ggccgatgag cttgcaaaag aatatccgaa
     6601 cagcttcatc ccgggtcagt tcgtcaatcc ctcgaacccg aaggctcact atgagaccac
     6661 aggtcccgaa atctttgagg acaccgacgg agaggttgat atctttgttg caggcgtagg
     6721 caccggcgga accgttacag gtgttggtaa gtatctcaag gagaagaagc ccgaggtcaa
     6781 ggttgttgcc gttgagccgc tttcatcacc ggttctttca cagggcgttt caggttcaca
     6841 taagattcag ggtatcggtg caggcttcgt tcccgatgtt cttgacacaa gaatttatga
     6901 tgagattatc cctgtatcaa atgaggatgc attcgcaaca ggcaagctta tcggcagcga
     6961 aggtgtgctt gtaggtatct cggcaggtgc agccgtatat gcggcaatcg agcttgcaaa
     7021 gcgtgaggag aacgagggca agacgattgt tgcactgctt ccggatacag gcgacagata
     7081 tctctcaact gcactttttg aataatatta cgaggttgtt aaaatgattt caacaagagg
     7141 aagatatgca ctaagggtta tgatcgacct tgctgagcac agggacgagg gatatatccc
     7201 gatgaaggac gttgttgcaa ggcagggtat ctcaaaaaaa tacatggagc agatcatgcc
     7261 ttcgctcgtc aggaacggct ttgttgaagg cgttcacggc aagggcggcg gatataagct
     7321 gacaagatct ccgagcgaat acacggcagg ggagatactc cgtgccgcag agggtgacct
     7381 cgctccagtt gcatgccttt cgtgtaacgc agaggaatgt ccgagaaaaa aggactgcaa
     7441 aactc
//
LOCUS       DAWBSW010000100         7425 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2007_length_7425_cov_2.641113, whole genome shotgun sequence.
ACCESSION   DAWBSW010000100 DAWBSW010000000
VERSION     DAWBSW010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2007_length_7425_cov_2.641113"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    309..331
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1895..1904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5851..5860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7079..7088
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1996 a         1502 c         1765 g         2109 t
ORIGIN      
        1 cgagaagtgt gcacgacgaa gtcgtgacgg agggagttta agtctgatga aacctcactc
       61 cctcagtcag cttcgctgac agctcccccg gaggaggagc ccacgctcaa tcacctgttc
      121 cgatttgtta acctaatacc aaaaccaaac gctcccttac atctgcaaga gagcgttaaa
      181 tttatattcc gttagattac caaacgatgt ttgtgaatgt ttccgtgctg acgatatcct
      241 tgacggaaac gctgccgaaa attgtgatat ttgcctggat atctgcggcg gcggagaact
      301 cggcggcann nnnnnnnnnn nnnnnnnnnn ncttgccggt ctttgaatcg acctttgcat
      361 aaacatagcc gtcgtgatag ttcatgttga tggacttgat gcccggaacc attttgcctg
      421 cttccgcagc gtcaataaca gacattactc tgccgtagct actcttttca cccttggcag
      481 gattctgctc tttgccgagc gtaagtctga tttcataata ctgaccgctt gttttgattg
      541 ttgcatttac aacatcctgc tctctgagct tgcttgacca ttcaaaacct gctgcaggga
      601 aatcgtcctt gatattgtcg ctgccttttt tcattgttgt atttttgttt acggcaacaa
      661 gcttgataag gctcggagaa ataccgtctg tacttccttt aacctctctt accatcttct
      721 tgttgtagcc aacctttgct gccttcgcct tgttgagagc ggtattgcaa tagttgatta
      781 tttcagcctt actcattact ttgcttccgc tcggctttga cgatgtctga ttgtttgatg
      841 agcctgcacc tgtctgtgac gagtctgatc ctgcgttgcc tgactgagtg ctgtcgggct
      901 gagcggcatc ggtctgattt gtacctgccg catcggttac ggcgtcaccg tttgcaccgc
      961 tgtcaatgat aggcgaatct gtcggtatac tgtctgtgcc gtaagaagca ccgccctgct
     1021 gaacctgtga tgaggtcgcc gtggctttaa ttgtgtctgc gaccttattg gtcgaaataa
     1081 ctgccgatat gcaaatgcct gcaacgcaga gaaccgcagt tgccgatttg ataagctgtt
     1141 tgccggtttc gttgttcatg gtagtaccct cctgtatttt tggtatttta ataatataat
     1201 tattttgcgc ttttgtcaat ccccctgata ttaaagagaa ttgatcatag agagaagctc
     1261 gacccaaaac agcctgccgt taaagttttc tctttgtgca ccttgaagtg ttgtatgatc
     1321 gcccttgata atttccatca cgttccaaac gccggtttta acgcttgaac tgtcatattg
     1381 aacagacggt gctccgatgg gagcaagagc cgaatatgta tttacaaggc cgtcgtttgc
     1441 ttgccatctt tcgtcaatta catatccgcc cggagttgtg cccgtgaagg agcaaaggat
     1501 ttttgctgtc tgagtatatt gagaagctat aagcttgctg tcggggaggc agttgccgtc
     1561 ctcgtcacgg tatgttccgt cgcaggcgta agaaaaatag taaatgtcgg aaacggtcga
     1621 gattctctcg ttgagagcca ttgcgttgtc aatcatcata tcgtaaagag cattatcgga
     1681 gaatttagga atgactgaag cggcggaatt aaagccgttc gtgattgccg ctgccgcatt
     1741 gtggctgacg caataagccg ttgttccgtt gtgaggagag gaaagagtta cgatgctgta
     1801 tatccagctt gacttgccgc ctgtgaaaag cggtgaaacg tcggagctgg ttttttcacc
     1861 ttcgtcaccg tttgccataa gctccgccaa cattnnnnnn nnnnttctta cggttgctcc
     1921 tccgaaggaa tgaccgaaaa gatttacttt tttctctgcg ctccaatcgt tgatgagacg
     1981 gcattcggaa taatctttgc cgaagcgggc gtgaccgcat tttttgctgt gagcttcgcc
     2041 gtaatcggtg acggtgccca tgagctgggc ataaagttca catgctctgt cccatgcact
     2101 gccggcagga gcaaccgtcg cggcatgaca atcaaagccg cgggcattga ggtattccat
     2161 gaggtcaccg tttctgactc cccagtacgg gaaaagcttg taaacatcgt cgtattctcc
     2221 ccagccggaa aagccgtgaa caaaaacatt tgtatattcc gtgttgaagc tttccttatt
     2281 gacatcggca tggggaatat taagcatcgg aaaaagaaac attattattg aaataaaaag
     2341 agaaataatt tttgtcatac aggacttcct ttcaaaaaaa cttgcatttt atttaagtat
     2401 atattattat ttatttaaga aattgtcaat tctcaaatga cgaaatttgt ccggaaaggg
     2461 cggttattat gcattacaga aaaaacggtg atactataag tgtaagactg agcatcggcg
     2521 aggatatcat aaagtccttg cttgagcttt gcgaaaaaga gaacatagaa tttgctcagg
     2581 taaacggcat cggagcagtc agccgtgcga ctgtcggttt ctacgatgtc aaaaagggtg
     2641 aatatatgcc gaaaactttt gacgagccaa tggagatcat ttcgctgctc ggaaacatga
     2701 caaggaaaga cggcaagccg tatttacatc tgcatgccgc tttctcggga gaggactgca
     2761 acgttgtagg cggacatctc aacgaagcaa ttatcggagt tacggcggag atttttgtca
     2821 atattatcga cggcaaaatg aaccgtcggg ttcatcctgt tacgggtata aatatttttg
     2881 acatttaaga aaaacatata aacggtttta acgcggtttt tcttgacaaa acgacaaaaa
     2941 tatcatatta ttaaattaaa aataaaagag ggcgtggtgt gtttatgttt aagtctttag
     3001 ttgctgcttg ggttgcattg gtcatggcat ttgtaagctt tgttccgggc tttgttgtgc
     3061 cggaggataa atccggtgag acggacaaaa gctaccctta tatttttgtt cacggatttc
     3121 tcggctgggg cgaggacgag ggcataaatc aggacttttc ctattggggc gcgacctcat
     3181 gctatcttat gcagaagctc agagaaaacg gcattgaatg tcgagatgct tctgtaggtc
     3241 ccttctcaag taactgggac agggcgtgcg agctttttgc acagttgacc ggcacacgtg
     3301 ttgactatgg cgaggctcat tcaaagagac acaatcatct tcgctatggc agaacttaca
     3361 cagagccgat tgttaaaaac tggggcgaga aggatgaaaa tgaccttatg aataaggtga
     3421 atcttatcgg acattccttc ggcggaaata caatcagact tttgcagggt cttctttcaa
     3481 aaggtgatcc ggacgagatt gcagccactg atcccaatga tatttctccg ctctttacag
     3541 gcggtaaagc aaattgggtg aattccgtta ccacgatctg cactccgaac aacggaacaa
     3601 cgcttgctta tatagccgat gaccttaacc ttatcgaaat aggtgaaata gctttgttcc
     3661 tatatgctgc cgttctggga cgttcaccgc tcaacggata tgttgatttt catcttgaac
     3721 agttcggact gacgtttgtt cccggagaga agacaacggt caaccatgtt tacaaagcgc
     3781 tgcatacaat actgcagcag aagtatgata acgttgcgtt tgacctttca tgtgaaggtg
     3841 caaaaggact taacgacagg atttctcttg atgataatgt ctattattat tcctatgcgt
     3901 tcagaactac cgtggacagc cctataaccg gtaaggcgca aatcgctttg ccgagcacac
     3961 tgtttattct ccgtgcatat tcggagatga tgggcgtata ttcctcaaat cccaatgcgc
     4021 cgttcccgat tgacgaatcg tggaagccca atgacggact cgttaacgtt gttgcggctc
     4081 aatatccgtt cggtgatgct catacggatt acgacccgaa taatatcaaa acaggaatat
     4141 ggaacgttat gccggtcagc acgggtgacc acggcaatgc gataggaata ggcgttgacg
     4201 aagaatcact gctcaaatat tatatggaca tgatagctat gattgaaaat caaccgatta
     4261 cggattgata atgattttaa tcattttgta catattgatt ttaaaaactg aataaaaaag
     4321 caccttcggg ataaattaaa accggaggtg ctttttaatt gaagcagtgt gttcggataa
     4381 tcagtatgat ctgttttttc gtttcgggcg ttattatgat gctcgttatt gtggggtatg
     4441 tccgtatccc ggacgaaatg acgattggcg aatatgatga aatgaatgtc ggaaaaacat
     4501 acgtctgtca agctcttgtg aaccatgcgg cagaggatgc ttctcccgtc gaaaccgaat
     4561 acatgacctc cgttaagctc tttaatgttt ttcccattaa atccgcaaag gttaaggtct
     4621 cacgccgaaa atacgttgtc cccggaggaa acgctttcgg aattcgcctg tatacaaaag
     4681 gcgtaatggt tatccgtatt gacgcagtaa caactgcgtc gggtaacgtc agcccgggca
     4741 agtcggcagg gcttaaagag ggagacatga taatctccgt tgacggtgtt gaagtctatc
     4801 gaaaccgtga gctttcggca atatttgcat caagtaacgg caaaactcga gcgggaagcc
     4861 aagacgagag agatcgaatt cactcccgtt ctttgttcgg aggactcaac ctacaagggc
     4921 ggtctttgga taagagacag caccgcgggg atcggtacgg ttacctggta tgacagaaca
     4981 aacggaattt ttgcgggtct cggtcacgcc gtgtgtgata cggatacggg agaaataatg
     5041 cccctttcgg gcggagatgc ggtcgaagct cagataaagg ggtgctataa gggcacaagc
     5101 ggcagcgccg gcgagctgtg cggagttttc tccgccggca ccataggaag cctttatatt
     5161 aacggcgaaa caggtgttta cggaataatg gattcctttg acgaaagcag tcgggtcgtt
     5221 ccggtcgctc ttcgtcaaga ggtgaaaacc ggtgctgccc aaatcatatg tactgtggac
     5281 gacagcggct cggattatta cgatattgag attgaaaaaa tttttgacag caaggataac
     5341 aggcgaaatc ttgttctgcg tgttaccgat ccagagctgc ttgaaaaaac cggcggcata
     5401 gtccagggaa tgagcggcag tccgatcatt caaaacggga tgctggtcgg agcggtcaca
     5461 catgtctttg ttgatgaccc tgccgaaggc tacggaattt ttgcgcagga tatgaccgat
     5521 accgccgagg gagtggagga gtatctcaag gtctcgtaaa ataatatacg ggcatatata
     5581 aataacggat aaaatgcttc aaataacatc aaactccgat tttctcggac gcaaaatgca
     5641 aatatggtgc aaaaatcggt taaacaaaaa gcgctctctt tcttgcaaaa gcacttgaga
     5701 gagcgtttat ttttcattct tcaacaacat ttttatagtt ttttcacaag gcataattgt
     5761 atcaaaaaaa tttttggtaa aaaatggcaa ataactattg acataaatgc cataaaatgg
     5821 tagtatatcg gtgaaacgaa atcacggagg nnnnnnnnnn cacggaggtg cgttttatgg
     5881 aaaataaggt caaaatacta attgcggaaa acaacgagtt cggtcagaac tgcgtcaggg
     5941 agttctcgtc ctacggctac aatgcattgc tttcggacaa ggacggcgcg caggttctga
     6001 ccaaaatgaa gtatgaaaag ccggatgtaa taatcatgga tgcattcatg cttcatgtcg
     6061 acgctctggg cgttatcaag cagatacggg agatgaagga cgggaaaaag ccgatgttga
     6121 ttgttctttc aagcgtggat aatatgcgtt ttgaaagtga aattctcaac agcggtgcag
     6181 attattattt tctcaagcct gttgatgcaa acataattgc tcagcgtatt acacagcttg
     6241 cgggctggaa aaatacgtgc acacatgaga gaacagagaa tgatcttgaa gttgtcgttt
     6301 ccgagatcat gcatcagata ggtgtgcctg cacacatcaa gggctatcag tatttgagag
     6361 aagcgataat tctttcaata aacaataccg agatgatggg ctctgtaacg aagcttcttt
     6421 atcctacggt tgccaagaca ttcaaaacaa cctcatcgag agttgaaaga gcaattcgcc
     6481 acgccattga ggttgcgtgg gacagaggcg acgttgatgt gttaagctca tattttggct
     6541 atactattca atcatcaaga ggtaagccga caaattctga atttattgca atgattgccg
     6601 ataagcttag attgagactg aaggcgtcgt aaaccaacgt ttatgctttg ttgacatttt
     6661 aaatgctctg tgttaaaatt tcattgttaa acatggaagg tgaatagaat gaaaaacaaa
     6721 gctcaaattt tagattcaag gaattataat atagatttat taaagatttc cgcgatgttt
     6781 cttgttgtgg ttttgcacat aacgggaatg ggcggagtga tagaaggcag tttaagattg
     6841 tcgacagata tatctcagaa gatcttgaac gctgttgcag tgtgttgcat caatttattt
     6901 tcgctttgta ccggatacct tctctgtaca aaaaccgtga aagtaaaaag gctgtttgct
     6961 ttatggcttg atgttttttt ctactcagta gtatgctttg cagcggctct tgttataagc
     7021 aaggatacaa gcctgctcag ctttccgtcg ctcggattct tgtttccgat ctgcttttnn
     7081 nnnnnnnngt acatgagcgt atattttgct gtttacattt tttccccgtt tttcaacagg
     7141 ttgattgaat cgcttgataa gaaacagttc tcgtggatgc tgatttgcgc tcttacggca
     7201 ttttcggtaa ttacgactgt tgcaggagag gatacatttc ttatcggagg agtatctgta
     7261 ggtcagtcct ttgtttggat aacaacaatg tattttatcg gagcgtatat caaaaaatac
     7321 ggattgaata taaaacgcgg aattcttttc gcggctgctg ctttgtcaat tatgtgtaac
     7381 gttattttta catatattcc ggaacagttc agatcctatg gggac
//
LOCUS       DAWBSW010000101         7297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2045_length_7297_cov_2.614333, whole genome shotgun sequence.
ACCESSION   DAWBSW010000101 DAWBSW010000000
VERSION     DAWBSW010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_7297_cov_2.614333"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2077 a         1434 c         1880 g         1906 t
ORIGIN      
        1 cctgaagtaa attaaatcac ggcggaaatc ggctaatccc gttttccgcc gtgttatttt
       61 gcaggtttat cattcttctg catcgctcaa ggcacggaaa accgtgcctt ttttctttgc
      121 gagaggttaa attgtacccg taaccgtgag aaccgttgca cccggagtca cattgtaggc
      181 gccggtcact gcgtcgcgaa cataggttgc cgccggaacg gtaactgcac cggcaactgt
      241 agcaaattcg cccgtttcgg cgttataagt gtaatccgtt gtcggcgtga gggctgttcc
      301 gccaagagtt acgccggtta tattgagaat cgggtcaaag gtatcgcgga taacaaggtt
      361 atctgccgct gttgcttcgg tgaagccgtt gtttctgata acaaaggtgt aagtcaaagt
      421 tccgttttcg ggaacggaaa gcgggcaaac tgcctttgtg atagagagaa gcggacctgc
      481 ttcaagcgta acggtttcct ctgccgtgac gggtgaggag agtcctccgc cgtttacgct
      541 gacggtgttt gtgatacgtg cttcgccgtc aagaggcgca aattcattca ccgttgccga
      601 gtaaacaaca gttgcgttac cgttggcagg aaccgtgatt cccgttattg tcagcggtga
      661 agcagccgta actgtcggct gtggctgaag atcgcctttg atgaaataac gcacactgcc
      721 ggcaacatag gtcagcggaa cgagtgtcgt tgtgttaaag gcgtatgctc cgagattatc
      781 ggttacggtc agaccggtga attccgtatc gcttgagttg acgatgttga tgatataggt
      841 tatggctttg tctctttcat aggtgtttga aagtgcattt tttgtgactg taagaacctg
      901 aacgatctcg cctgtaacga tgttggaatt gataacgtta tcgttatagg tcaaggtcgc
      961 ttggtttgtg aaaagagcca ttgcaagacc tcctttgttt ttttgaaagc ttttgcttcc
     1021 gttatattat atgaaacgaa aaataatttg ttattgttca aaataatcgg taattactgt
     1081 atttatatct ttatttttta ttttttgtgt gatataatca ttctgtaaaa ttatgtttac
     1141 gcaaagtcag cataaggagg ataatcaatg gcacatttta ttttttccgc tttcagcgac
     1201 gaatccggtg aaagtacggt aaaaggtcaa attgcagcat gcaaggagaa cggcatttcc
     1261 gaaatggagc ttcgcggttt cggcaaggag cttaacatca acaatatgac tgttgatcag
     1321 gccaaagaga tcaaaaaaac aattgacgat gagggcatga aggttgcctc gattggctcg
     1381 gcttacggaa aaatcaatat caaggacgat tttgaaccgc attttgaagc atttaagaat
     1441 acggttgaag ttgcaaaaat ccttgaggct aaatatatca gaattttcag cttcttcttt
     1501 gacaaagagg acagctatga tgattataag gaagaagtgt tccgccgagt taaggcaatg
     1561 gttgactatg cagacgaaaa cggtataatg tgctgccatg aaaatgaaaa aggaatattc
     1621 ggcgacacgg cagagcgctg ccttgaggtt cttgaagcct gcggcggtaa attaagagct
     1681 gtttttgacc cggctaactt tgttcagtgc ggcgtcgata ctctcaaggc ttatgaactt
     1741 cttgaggatt atatcgaata tatgcacgtc aaggacgcaa tgtattccga cggtcaggtt
     1801 gtccctgccg gagagggtga gggaaatgtt gaaaagataa tcggaaagct ctcattcaag
     1861 tggaaaagaa ttatcctttc gcttgagcct cacctcaaag tgtttgaagg ctttgactcg
     1921 cttgaaaacg atgacgaaac caagaagggt atggacaagc atacctatgc ttcatataaa
     1981 gaatcattca aggctgccgc agatgccatg cacaggcttg ttgaaaaagc tcagcctatt
     2041 cgcacgggta ttgtcggtgg aggtaatatg ggatcgagcc atatccgcaa tttccttgac
     2101 ggcgaaatga gcgagatgag agttacggca gttgccgata ttaaccccga gagacttgaa
     2161 tgggcgaaga aaaatgttcc ctgggctaag agatattcaa ctgccgagga gctttttaaa
     2221 agcggtgagt gtgacgctgt tataatcgct gttcctcact atttccatcc gccttatgtt
     2281 attgaggctc tcaaaaacgg actgcatgtt gtctccgaga agccggcggg cgtttataca
     2341 aagcaggttc gcgagatgaa cgagtttgcc gctaagagtg acaaggtgcg tacaaactgc
     2401 gttttcagaa agatgaaaga aattgttgac agcggaaaat acggtgaaat tcgccgtgta
     2461 aactggataa tcaccgactg gtaccgcacc caggcatatt ataattcggg cggatggcgc
     2521 gcaacctggg caggcgaggg cggcggcgtt ctccttaacc agtgtccgca ccagcttgac
     2581 ttgtggcagt ggatctgcgg aatgccctca aaaatccgtg cattctgcaa cgagggcaag
     2641 tggcatgata tcgaggttga ggacgacgtt acgatttatg ccgagtatcc gaacggagca
     2701 acgggcgttt tcatcaccac gacgggagat taccccggta caaacagatt tgaaattacg
     2761 cttgataagg ctaaaattat atgcgagaag gctgagaaaa tcacgcttat cgagcttgag
     2821 gaaggtctga cacacaatat acaaaatgcc aaggacggct ttgtaaggat cggaactcat
     2881 actgttgatg ttgagaccga cggaaggaat gagcagcatc ccggagttct caatgcgttt
     2941 gcaggaaaga ttcttcacgg aactccgctt gtcgccgacg gcaaggaggg cattaacgga
     3001 ctcacgattt ccaatgccgc tcatctttca agctggacga atgaaacggt ttccattcct
     3061 tttgacgagg ataaatatta cgatttgctc atggagcacg ttaagaactc caaagcaaaa
     3121 acaaacgttg tcgagcaggt tgccgacgat atgagcgata catactgagg tgttttatga
     3181 gggtttctgt tataggctgc ggcaacattg cgcaggttca tttggagata cttaattcca
     3241 tggagggaat cgagatttcg tcggttgccg atatcgttga ggaaaaagca gacaaggctg
     3301 ccgaaaaata cggttgcaag gcatactatg attatataaa aatgcttgat gaggatcacc
     3361 ctgacgccgt gcatatctgc actccgcatt atcttcatgt tgaaatgtcg gtcgaggctc
     3421 tttcaaggga tatccacgtc ctttgcgaga agccgtgcgc catgaacagg gaggagcttg
     3481 cgaaaatccg catggctcag ctcatgagca cgcatgaata cggcgtttgc ttccagaacc
     3541 gctacaattc gtctgtaaga gcaataaaaa atactcttga aacagggaaa tacggcgcaa
     3601 ttaagggcgc aagagcggtt gtccagtggt gcaggaacga ggattattat tccgatgatt
     3661 ggcacggaac gctcagaaaa gagggcggcg gtgttgtcat aaatcaggga atccatacgc
     3721 aggatctgct tcgatatctt gtcggaagcg atgttgtcag cgttacggga catgttgcca
     3781 acgaccattt gaaggacaaa atcgaggttg aggacactgc tcacgcaagg tttgtttttg
     3841 caaacggagc aataggtctt tatgatgcaa cgacggcttt cagccttaac gcaaagccta
     3901 taattgatat tttctgcgag agagcaacgc tcagggtcga gggcgacaat gcctgcgtta
     3961 tttataacaa cggagaaatt gagcttctcg gcaccgaaag cgatatcgca agaggtaagg
     4021 attactgggg cggcggccat gaggctctta tacacgactt ttacgattgt attgcctcgg
     4081 acagcagatt cccgatagat tcccatgagg gcggcaagtc cgtcgaggag cttgcggcta
     4141 tttatgagtc ctctgaaagc ggagaggaag ttattctcgg aaagaggtga tcttatgcag
     4201 atgacaatgc gatggttcgg cgacggtaag gacaccgttt cgcttgagca gataagacag
     4261 gtgcccggcg tggcaggcgt tgttcctgct ttgcacacct tgccggcagg agaggtttgg
     4321 ccgcttgaca tgattcttga tatgaaaaaa gagatcgaag cggcagggct tacaatggaa
     4381 tgtattgaga gcgtcaatgt gcatgagagc attaagctcg gtgacggcga tgccgataaa
     4441 tacatcgaaa actacaagga gtgtatccga aatctttcca aggcgggagt gaaggtgatt
     4501 tgctacaatt tcatgcctgt ttttgactgg acaagaaccg atcttgcaat ggatatgggc
     4561 aacggcgcaa cttgtctttg ctatgacgga aagcaggttg agggcaaaag ccccgaggat
     4621 atgttcaggg agatcgacag caactccaac ggctatgcga tgccgggctg ggaaacggag
     4681 cgcatgggcg agatcaagga gctgttccga aaatataaaa acatcacgca tgaggatatg
     4741 tggagaaatc tcaggcattt cctcgaagaa atcattcccg tttgcgagga atgtgatgtt
     4801 aaaattgcta ttcatcccga tgacccgccg tgggatattt tcgggttgcc gagaattata
     4861 aaaaatattg atgatataag acgttttctt aatcttgttg attcgccgta taacggactg
     4921 acgttctgca caggctctct cggagcgagt acggataacg atttgccggc gatgataaga
     4981 gaggttggcg acaggattta ttttgcccac ctgagaaacg taaaaataag agaccggttc
     5041 aatgaaacct cacatctttc ctcggacgga agccttgata tgtatgagat agtcaaggcc
     5101 ttacaggagg tcggatttga cggctatgta agacccgacc acggaagaat gatctggggc
     5161 gaggttgcgc gacccggcta cggactctat gaccgtgcgc ttggctgtgc atatctcaac
     5221 ggactgtggg aagcagtcgc aaagaacaaa aaatcggagt gatattatga ctaagcatga
     5281 aagtcttaac ggaaaagttg cggttataac cggcggcggc ggagtgcttt gctcaggctt
     5341 tgcaaagacc cttgccgagc agggcgtaaa ggttgccgtg cttgacctta atgaagatgc
     5401 ggcaaaaaag gtcgccgacg aaataaattc aaacggaggt acggctattg ccgtcggctg
     5461 caacgtgctt gaaccggaga gcatgaaaaa ggcaagggac attgttgccg aaaagctcgg
     5521 cacatgcgat attctcctca acggtgcggg aggaaacaac cccaagggca cgacgacaaa
     5581 ggaaacgctt gaaaaaatcg accttgttga aaaggacgag aatgtgaaaa ccttctttga
     5641 tcttgacccc gagggaatat cgttcgtttt taatctcaac ttcctcggaa cgctcattcc
     5701 cacgcagatc tttgcccgtg acatggcgga aaaagaggat agctgcattg ttatgataac
     5761 ctcgatgaac gctttccgcc cgctgacaaa aattccggca tattcggcag caaaagcggc
     5821 ggttgcgaac ttcactcagt tcatggcggt tcattttgcc gatatgggta tcagagtcaa
     5881 tgcgattgca cctggctttt tctcaacaaa gcagaataag gatcttttat ggaatcccga
     5941 cggttcgccg acggcgagaa caggtaaaat tcttgccgca acgccgatgg gacgcttcgg
     6001 caagcccgag gagctttccg gcgcattgct ttttctctgc gacagggagt attctggatt
     6061 tgtaaccggt acaacgattg ccgttgacgg cggctttaac gcatattcgg gagtataaac
     6121 cggaggtgat aaaatgtcag cgcttgtcag cgtagttgtt cctgtataca atctcgaaaa
     6181 atacatagaa aattgtctca aaagcattgt cgctcagact tataaaaatc ttgaaatact
     6241 ctgtattgat gacggcagca ccgacgggtc tgctcggata ataaaagagt ttgccgggac
     6301 tgatgagaga ataaagtatt tttatcaaag aaatcaaggg ctgtctgcta caagaaacga
     6361 aggtattagg atttcaactg gggaatatat tttgtttatt gatcctgatg attttttgca
     6421 cgttgaagcc gtggagagat ttgtgcaagc ggctttggaa gaaaatgccg atttggtttg
     6481 cagtgaatta aaatatgttt ataataacaa aataagtgta aatccaaaaa tttctgaaag
     6541 ggatttttat agtatttcta ttgcagatat gttttataaa tataaatgcg gttacatggt
     6601 caacggaagc ctgttcaaaa gagatttaat ttttgacaaa cggtttatgg aatcaataaa
     6661 aaacggcgaa gacagcatct ttatgcttca agttattctt tgctcaccca aaaccgtgtt
     6721 tattgatgac gtttgttatt actattatgt acgtgacgat tcgctttcaa gaaatataaa
     6781 atataatatc ggcatggctg atattgtcga tgcggcctcc atatgctatg aacttgcaaa
     6841 ggaagcagga aacgacactt tgatttccgt ttaccttagg ggtatataca gcacgttgtt
     6901 tgaacataga ataagatgta agcattccga atgtcaaaag aagttgagcg aagaattaaa
     6961 tcaaccggca agaaatattt aaagagttta tttgaagatc cgaatatttt gcagactgaa
     7021 aaatttgcaa ttgctgtttc ttttaaaatt ccgattatgt ataagttttt cagaatatta
     7081 aaagacccgt caatgttcgg tatttatttg cgaggaaaac tatgaagaaa accgtcagta
     7141 taataattcc ggtttataac cttgaaaagt acatagtcaa ttgtttagaa agcatttttg
     7201 cgcagacata tgaaagtctt gagattattt gtatagatga cggaagcaac gacaaaagcg
     7261 cggaaattat aaaaagattt gccgcttcgg atagtag
//
LOCUS       DAWBSW010000102         7273 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2057_length_7273_cov_3.094209, whole genome shotgun sequence.
ACCESSION   DAWBSW010000102 DAWBSW010000000
VERSION     DAWBSW010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_7273_cov_3.094209"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4078..4087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6699..6708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2061 a         1587 c         1603 g         2002 t
ORIGIN      
        1 tcaagccttc tgccctcgtt gtaagcctta acaaccgcct gaactgaagc gtcggtatag
       61 gtgctgggcg ggaaaagatt tgcaacacgg ctgatctgag catcaagctg tgaataatcg
      121 gcaagtccga tataattctt caacgaatga taagccttcg aaagctgacg tcttgccgta
      181 ttgatcttgg actgtctgta atccataaca gttccggtag caaccttttc gttatactcg
      241 gtcataacct tgtttgcaaa ctggtatgcg ttccagaatg cattccatgt tctgtcggta
      301 tacttcttag ttccgtcagc gttgagatta tcgttgtaat taacggttcc gacaacatta
      361 agaacctttt caagctgata tgttgacttg tatgtctgat ttcctataag gagatccttg
      421 tacttcataa gagcatagca actgaaagta acgtctgcac ttgacgggaa cagttccttg
      481 cctatctttg cctgctcata ctgattaagg atatttcctg cttctctgag tctctgctta
      541 aatgcaagat atgcccatgg cgcaaagtca accatgtgga agtattcata attatcgtcg
      601 taataatgaa gtccttcatt tcccgcaaga gcaagctcat catatactgt tttgagctcg
      661 ctgatgctgt aggtctttcc tgccggatga ttgttgttgt aatcaaccgt tacataatca
      721 taactcttga ggttaagaac accgagaagc tcggcaagaa tatcaagcga tgtgtcgcaa
      781 agtgaacccg gacccgtaat attgccgagg cttgcaagaa gctgcttaac agtattcttg
      841 agtcctgtac cgttattaac tccgtcggaa tttacaacag taataacctg gtcaagcttg
      901 gtatagctgt aaggaataat aaggccaacc tgagctgcat tatcggttgt tcctgccgtt
      961 tcatagtgag gaagctggaa caagccgtta atagctcttg caagaaggtt tgtgataatc
     1021 tgcgtaagcg gcttatcaag agttgctgta agcttaggtg tcgaagcacg gcgaccgata
     1081 agtgaaagga ctgtgttgat atcaaagtca aggatagcgt tcaagagttt atccataacg
     1141 atttctctga aaccgtcctt attattctct acaccgtaaa gaatgttagt gagaggaata
     1201 atctggttaa ccgaatagta aacctttgcc caaacgtcgg taccgttggc tgccgagccg
     1261 agattgaaga actttgaata ctttgtaagg aagaaatcaa atgctgcgtt aagctctgcc
     1321 gagaaggtaa tctttgtagg atccgaatta actgtctgca tgccgaactc accgacaagg
     1381 tagtagtcaa ccaatgcacc tacaaggtca aggcaccagt ctccgtcagg attgtactgc
     1441 gaaccgatat agttgccgtt ctcatccttc ttagccatga gatcctcaaa ctctgcgttc
     1501 ggatagagat cctttaatac gtcgataaga gtaaatgttg caagctctct gatagaattg
     1561 cagttgtccg agaactcaac atcatcaaca agagcctcaa gaaggaactt aacaaggaat
     1621 gcgaacgtct gctcgtcatt catgttttca agagcctttg actgctcgtc tgtaatttcc
     1681 ggagcgtcct tccagaagga gcctatgatc gggagcaatg tcttaacata tccggagagc
     1741 tgcgatacaa agcccggctc aagtgtaaga tactttccgc cgtttccgtc atctttaaag
     1801 tagatgtact ttgtaaggcc ttccttaagg atatattgaa gtgtaacgtt tatcttatct
     1861 atcggcttcg gggctgtcgg attttcaacg cccttcgact caaggaattt ggcgatcaat
     1921 tcatcctgag atgcatcttc gggaagaccc attgtctcat tagtaacgaa aagggttatc
     1981 gcgatattga tataagatgt gtctgccgtg gtatcgtccg gcttgatctt gagtgcatca
     2041 agaataaggt taccggcata cttaacaacg atatcatcaa taagcgcatc gaaaagagcc
     2101 tcaatgaaat catatgtctt aacctttgta aagtcgatcg aaggtccgat tgacgtcttg
     2161 agtgacggaa gaagatcaac gaatttaacg taattcttca aggtaccgtc ggcgttcttt
     2221 tcaagtccga ggaaatcagc aacagcattt gagccgtcag cctttgccat cttatcgcag
     2281 acaaacttga caagacggtt gttaaagaaa tcattaacta tgtagtcgag gttgtcataa
     2341 gctccgccta ccgcaaagcc tgtacctgtg gaatcaatca atgccttata gaccatctcg
     2401 ttgatatagc cgtgaaggtc gttaagcata ggaatctttt tcttcggatt ccagaagttc
     2461 tttatgatac caagattgaa cgagttgtct acccatttat aaagcggatc ggcattctcc
     2521 tggagccact gaagaagata tccgagaacc tcaagatcgc tcttgttctc tgaacggcgt
     2581 ctgatgtcac cgttcttcgg gattccgaag ctcaggttat caatgtctcc gaagctgaac
     2641 attttataaa gtgtgtgtcc tgtaatatcc ttgattgaat caaagagagt gtcaagagaa
     2701 tagatgtgaa tatgaagact tagctggctc aagtcaaagt ttacatccag tcctgcaaga
     2761 acctcatcat cgagatagtc aagaacagcg gaagccgcct gctcttttgt gaaaacaggc
     2821 ttttttacac tgctgtagct acaggtattg tatggggttt tgtatgcgct tacgcctacc
     2881 gtgaagcatg aaagcaacat gacaagggca ataagaacgc acaacagtcg tttggattgt
     2941 ttcacctaaa acacctccgt taaatttccg gcctacgacc ggagaaactg taaatagggt
     3001 cgccaaaatc caccattcca gtttggctta taataatatt atcagaaaag ccttaatttg
     3061 taaataggtt tttccaaatt aaaatattac aaactgtaca aaatttctta ttatttttat
     3121 atgaatacaa taaacaatac aaaataaatg tataaacaac agaaaaaaca cagcatttta
     3181 gtcaattata cggctgtgtt ttcttcttga tctgcaagtt ctcacatacc ttttataata
     3241 tataatctgc accgtcatga catttttcta tattttatat tgatttcagg ttgaaaaagc
     3301 aaaccgatat ggtataataa cctcaaccaa ccgtttgtta aatgagcaaa gaagtttaaa
     3361 gaggtgctat atgaaaaatt ctgttaatat tggaattatc ggcctcggag gcagaggcac
     3421 aagtatgatg agtgtcatgg caaagatgga ggatatcaat attcttgccg tttgcgacaa
     3481 gtatcccgac agagttgaca atgccgtaag aattcttgtt gacagcggca gaccggctcc
     3541 gatgggcact gttgattaca acgaggttct cgatatgccg gggattgact cggtttacat
     3601 tgcaacggat tgggaatgtc acgttaaaat cgccattgat tcaatgaaaa aaggcattcc
     3661 ggctgcaatg gaagtcggcg gggcttacac cctcgatgaa tgttgggagc ttgttcatac
     3721 atatgaggaa accggcgttt ggacaatgat gatggaaaac tgctgctacg gcaagcgtga
     3781 gcttatggtt cacaatatgg ttcgcgaggg tgtattcggc gacattgttt tctgctcagg
     3841 ccagtatgcc cacgatctcc gagacgaggt cgcctacggc gaaaagaacc gccactacag
     3901 gcttcgcaat tacattgcaa gaaactgtga aaactatcct acgcacgaat tgggtccgat
     3961 tgcaaagcta ctcggcataa actacggcaa ccgttttgtt tccatgtcaa ccgtttgcag
     4021 caaagccgca ggcatggagg acttcgttaa acagcataag gatctcaaac cctctctnnn
     4081 nnnnnnnctc taaccctctc tcggcagcac gaaattcaat cagacggaca ttgtcaagac
     4141 atttagtaaa accgaaaaag gagagcttat cgagcttact cttgacacct gtctcccccg
     4201 tttctacagc cgaggcttca cggttcgagg cacaaaggga ctgtatcagg aagacggcga
     4261 catcgttttc ctcgacaaga agctatatag aaaaaacgag tgggatcaga aaaaattctg
     4321 gggaagagga aaaaaattcg cacgcaaata caatcattcg ctttggcacg acaccactaa
     4381 ggaaatgctc gatgcaggtc acggcggaat ggattggttc attataaggg cctggatcgt
     4441 atcggttaaa aacggcactc tcccgccgat tgacgtctat gatgcggcag cgtggatgtg
     4501 cattactccg ctgagcgagc tttcaattca gaacggcggt gcaaccattg agattcccga
     4561 ctttacgaac ggcaaataca agaacagaac ccccaaaaac gacaatatat atgatataag
     4621 ataattgagg aggataagaa atggtaaaca aactgaccgc tgtcattgtt gcgataacaa
     4681 cagtaattat gtccgtaaca ggccttttcg tatggggctt tgcacccgag gatgaaaccg
     4741 agttcacccc ggtgattcgt tttgtcgcat gcagcgactc tcatcttaaa actgccgccg
     4801 gcgtcggaag ccacaggctc gaaaagacgg caaagctcgc ttatgctatt gcaaaggcag
     4861 acgatgaata caataatctt gatgcatttc ttatcaacgg tgatataaca aacgacggca
     4921 atattgacca attcgcaggc ttcaaggtca ttgccgacaa gtatatcaag agtgagactg
     4981 agcttctccc gataattgcg aacaaccacg atttcagaaa catgaagaag cagaccgttg
     5041 cctatttctc acagctcatg ggcaaggaca cagactatca caaggtagtc aacggttatc
     5101 attttatcgg aatttcgact tccgacgttg agggtgtgaa ctacaccgag gcgcagagag
     5161 tatggctcaa ggctcagctt gacgaagcgg ttaaggacga tcctaacaag ccgatttttg
     5221 tgtctcagca tgagcatgtg ctcaacactg tttacggcag ctcggatttt gacggctggg
     5281 gaattgacga tttcagccac attcttgaac agcatccgca ggttgttgac ttttcgggtc
     5341 actcccacta tccgcttaac gatccccgtt ctgtatggca gggcagcttt accgctatag
     5401 gaacaggtgc tctcgcatac atggagctta cggttgagaa tcagcgcaag gttcacccga
     5461 caggccacag cctcgaagct cagttctgga tcgttgagct tgataaggac agcaatatgc
     5521 gtctgagagg cattgacctc attgagcaaa aggttctatg cgaatatatt cttgccaatc
     5581 ccgcaaatcc ggcaaaccgc gaatacactc cggaaaagca ggcagcaaga tcgaccgcac
     5641 ctgttttcag tgaaaatgct gagctcaagg tcacaaagct cgcaaagagc gttacggtga
     5701 aatttgatgc ggcacagtcc gcaagctctg aacctatttt cttgtacaag gttaaggtca
     5761 taaactccga cggcgaggtt gttaaatccg actgggttct tccgaagtat tattcggcaa
     5821 cggttgagaa aaccgagcgt ctcaaactcg gcaaatttga agaaggcgat tatacggttt
     5881 ccgtcacagc cgagactgct tggggcgtac agtcggagtc tataacgaca gatttcacgg
     5941 ttaaataaat tatggcgact ttacttttaa ttgttatctt tattgatttt atcgggctcg
     6001 gaattcccga ctcgcttttt ggagcggcat ggcccgcaat caattatgac tttggtctgc
     6061 cgatttcggc ggcgaatgcg gtcaccgtca caatgacggt atgctcgata atttcaagtc
     6121 tgatgagtgc aaagctgaca gaaagatttt cgacgtcaaa aattgcagcg gtgagcacag
     6181 cgcttaccgc tgtcggtctg ttcggctttt caatctcaaa gaacatatat atgatgttct
     6241 tcttcaccct gtttctcgga ttcggtgcag gagcgataga tgcagcactc aataattatg
     6301 ttgcagttca ttataaggca tcgcacatga attttctgca ctgcttctac ggaataggcg
     6361 taactctcag tccgtacata atgtcgcttg cgctcaagaa ccgatcttgg gaaagcggct
     6421 accgctgggc atctattatt cagcttgtca tttccgttat cgcttttgcc tccctccccc
     6481 tttggcaaaa gaacggcata ttatcgggtg tttcggaaga aaacagcaaa agcagctttg
     6541 cagagcttat aaaacttccc ggagttaaaa caacctggct tgtgcttttc ggttcatgct
     6601 cgcttgaata tgtcagtggt acatgggcaa gctcgtttct cgtcaattcg agaggactta
     6661 cggctgacaa agctgcgcta tttattaccg tctactacnn nnnnnnnnta ctacggcgga
     6721 atggcattcg gacgttttct ttcggggatt ctctcgacaa aacttaaacc gcagcaaatt
     6781 attgcaatcg gtacaattct tataattccc gccattgctc tcgtttcaca accgtttgta
     6841 ccatttctgt gtgcagcagg actattcctt atcggactcg gcaacggtcc tctttatccg
     6901 aacatggtgc atctaacccc aattcgcttt ggcaaaaaaa tgtctcagat ggtcatgggt
     6961 tcccagatgg cggcggcata tatcggaatt ctttccatgc ccgcactgac aggcttcctt
     7021 gctcagaagt tctcaaccga tatttttcca tattgcttga ttatcttata cggcataatg
     7081 cttatttccc ttatttttac tcgaaaatca aaaattgtaa atgaataatt tattaaatta
     7141 aaaaatttgt taataaaaaa ctattgatta tttaataaat agctgataaa gtcaaagtaa
     7201 tcataaatac aaacagaaag gacgttatgt atgaaaaaga tactttctat tttgctttcg
     7261 gcactccttg cgg
//
LOCUS       DAWBSW010000103         7160 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2092_length_7160_cov_2.745813, whole genome shotgun sequence.
ACCESSION   DAWBSW010000103 DAWBSW010000000
VERSION     DAWBSW010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_7160_cov_2.745813"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4530..4539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5279..5305
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5515..5524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6297..6306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1909 a         1546 c         1630 g         2018 t
ORIGIN      
        1 aatggagccg tacaaaaaca gcgatactca gtgtacggtt gaaattgacc ttaaaaagga
       61 agaaacaatt cacttcgttg aaatcagcga ggatatagct cggggacaga ggatagaaaa
      121 cttttttgtt cagcggtata agaataacag gtgggaaaac tgttttgagg gaaccaccgt
      181 cggcgcaaga aaaatcgtcc gttttgacga gggtcttatt gccgacagac tgagaatttt
      241 tgttaccgcg gctcgggata tgcccgagtt caaaagaatt gcgatttact gataactgtt
      301 gaaaaaacgg aggtatgtta tgaaaagggt gcttgatttt ttaatcagcc tttttgcatt
      361 gattattctt tcgccgttgt tccttattgt ttctgtcggc gtcttgattt ccgacggcag
      421 ccccgtgttc tttcgccaaa aacgcgtagg aaaaaacaac gagctgtttg aaatatataa
      481 attccgaaca atgaagcgcg gaacggaaaa cgttgccagc aacgatcttt ccgatgccga
      541 tgttaagata acaaggttcg gaaaaattct tcgtgcaaca agtattgatg agcttccgca
      601 acttctgaat attctcaacg gttcgatgag ccttataggt ccgcgtccgc tcattccgga
      661 ggagacaaga atcagagagc ttcgtgagaa atataatgtc tacagtgttc gcccgggaat
      721 aaccggttgg gcgcaggtca acggacgtga caatgtttcg gcagagaaaa aggctctgct
      781 cgacaaggaa tatgtcgaaa agcaaagcct tatgcttgat attaaaattt tctttatgac
      841 gattcatcag gttctttgcc gcaaggacgt aaacgaagga aacaacagaa cttaatttgc
      901 gttcaggtat tcctcaagcg taacaccttt tgccgtgata tcctttgcgg cctcaacgcc
      961 gacaaagcgg taatgccacg gctcatagat aaccttggta atggcacggg attttgcgtc
     1021 tttcggatag cggagtatga atccgaaatc agcggcattt tctctcaacc atgctgcctc
     1081 atcggtatcc tcaaagcttt cggaaagcga gcagatatcc attgcaagac ctgcattgtg
     1141 ttcgcttccg ccgggaatca ttacctcggt tgcggcagca acggttgcct ttgttttgtc
     1201 aagaccgttg ttatcaattt caagctgaat ttggtcttcg aaaagctcga cctgaagatc
     1261 atatgagcgg tagccggaaa caggtgtcag ctcaattccg tcctccaagg ccttgtcata
     1321 cattgcctga tagtacggcg caacacggta atcgagatat tttcccgagc ctttaacggc
     1381 ttcggcaagc gtcggttcat agccttcagg caaaacatgg gtgccgttta gaagaataga
     1441 tgaaagcgaa gaatttgcgt caatattgat tatttgagga ttaaatcccg cgtaagacta
     1501 tttataactg ctgacggtgt tcaacttctt ttcggttttc gtttcgccga cagttgtttg
     1561 tttttcggcg gacgtgcctt cattcttttt ggacaattct ccggaattgt tcgttgtgtt
     1621 cgcccccgta tttgtggtaa tctcaacttg tgcatttact gcttttccgg tgtttttata
     1681 ttcgctgtgc acatttattg ccgcacctac ggttgtaaca gcgacaatga gaatgattga
     1741 ctgaataaca atcagcttta ttacacgagg tgtgattttt ttattcattt aagatgtcac
     1801 ttccttgata ttttatattt ctattttatt cgtttattga gtcattgtca acaatgcttt
     1861 ataaaattat tattatttga aaggaaagat tccaatgaaa gcatcggaaa gcgtagagag
     1921 atacgattct gaagtacgtc tctataccaa ctacgcaatc aaaaacatta aaaaggtctg
     1981 taaggacttc ggcccccgtc cggtgggaag tgaggcagag aagaacgcgc agcttcatat
     2041 gaagaaggag cttgaatcct catgcgatac cgttacaaga gaggagtaca aatgctcgga
     2101 taaggcgttt atggcttggg ttccgctcgg agcaatactt attctctttt caattgtaat
     2161 gttcacgctc ggcattcccg ttgcgtcgct tgcagcttca cttgttacgc tttttataat
     2221 tcttgcagag ttcattttct acaagcccgt gcttgatgtt ttcttcccga aaaaaacatc
     2281 cggtaacgtc atcggtgttc gcaaggcttc gggcgagaca aagaagcgta ttattattgc
     2341 gggccataca gattcggctt ttgaatggac atacacctat cacggcggac acaatgcggt
     2401 tcttacaatt attctcacgg caattattgc gattctcctt ggcatcggcg gaagcatcta
     2461 tgctcttgtt gcaggcgctc agggcgttgt atggacaggc gataaccttg caatgaagat
     2521 tatcgcagtt gttacctatg tgacggttcc cgttatttgt gcggctattg ttttcacaaa
     2581 ctataagcgc ccggttatgg gagcaaacga tgacctttca ggctgtttcc tttcggcagc
     2641 ggttgtgaag ttccttgccg caaatgacat tcgttttgaa aatacagagg ttgttgccgc
     2701 tatggttggc ggtgaagaag caggactcag aggaacaaag gcttatatga aggctcatgc
     2761 acaggagttc aaggacgaaa aggatgttga aacgatattc gttgcctttg atactatccg
     2821 tgaacacgaa tttatggcta tttacgataa agacatgacc ggtatggtaa agaacgacaa
     2881 gagagttgca aagcttcttc aggatgcggc aaagagtgtc ggctatgatg ttccggtcaa
     2941 ggctatagag cttggctcaa cagatgcagc cgccgcttca caggcgggta tccctgcggc
     3001 ttcatttacg gcgatggatc ccgctccggc aagatattat cacacccgtc ttgacacaga
     3061 ggataatctt gatcccaaga cccttgaaat cggactcaag attgctcttg aaacagtatt
     3121 cgattttgac gagaagggca tctgacagaa atcaatcaca tttcccaaat attttcctta
     3181 aaactattgg atttttgaaa ttattatggt ataattacat agtgaattta ttattgctca
     3241 aaaggagttg gcattgtgaa taaaaaaatt gtaagatttt cgtcactctt gcttgcattg
     3301 ctcatggttc tttccctgtg cgcttgcggc gaggaggaag aaacatacga ttacaagtca
     3361 cccataccta caactacgga agagatcctt tcgagattta acgacgcaat ggcagacgct
     3421 aaaaaaggca accccggcat cagctatagc ataaagcagg gcgcaaacat gagcggcgag
     3481 cagaaagaaa agtgcgaaaa cgaatacgtt aaggctgctt tcaagacagt ttcaaaggct
     3541 atcaccaagg agaacttctc taacgagact gcttacggcg agagcacaaa ggatattttc
     3601 ccgacattcg gttcggatga tgcacccgta ctcaccaatg ccgatatccg ttcagcttat
     3661 gttatggaga ataaggatga cggtacaggc gcaacttaca taatcgttat aaagatctat
     3721 ccggagaagg atcctaacca gaaggacagc atctacggaa agctctataa tatcatggac
     3781 gacaaggaaa ttttgtcaca ctttgatgtt attaacagcg ttgtaacggt taattcttac
     3841 agcgcacagt acggcgaggg aacgatccgt gcaacgctca agaaggaaga taataagctc
     3901 accgagctca gtctttcaag gaacgttgtt gtttcaacag agcttacggg cgtaggtact
     3961 cttgccgatc tcggaactgt tccgcttgag ttcaattatg aagcaacgga aaactacagc
     4021 gttgactggt ttgatcctgc aacaaaggac gcaaaataaa ctgattgata aaaaattacc
     4081 ctcggcggag cgattccgct gagggttttg tcatgttatg gttttatttg caatagtttt
     4141 ctatgtaatt atcaattgct tcggagataa ccttgacggc aacactgcgt tcactgacct
     4201 cattgacggc tgtttcctta ttctcaagcg ttttgtaaac cgaaaagaaa tggcgcatct
     4261 cgtcaaaaat gtgctttgga agctggtcaa tattggtgta aatattatag ttcgggtcat
     4321 tgaacggaat tgcgattatt ttttcatcgt tgcgtccgtt gtcaatcatt gatataacac
     4381 cgatcggata ggcgcggcaa agggtgagcg gttcaagcgg ctcggaacaa agaagaagca
     4441 catcgagcgg atcgccgtca tcgccgagcg atctcggaat gaaaccgtag ttggccggat
     4501 agtgggtcga ggtgtaaaga attcgatcan nnnnnnnnna tccagccgca gcagaccggt
     4561 ctgcttgtcc agctcatact ttttcttgct gccctttgga atctcaacaa cgcaaacaaa
     4621 atcctccggt gaaattcttt ttggcgaaat gtcatgccat atattcatgc gttcacgctc
     4681 ctttaatttt atagtaactt atttgacacg aattgtcaag caacagatgt atcagagaac
     4741 aagttcgttt gttccggtgc tgagagaaat ttttttgcag tcgccgataa aaacagcgtt
     4801 gataccgtca cacaagtcaa tgaaaatctt aatttttttc tcacatcgct tccatgcaac
     4861 cgcaatctct ccgtgaggcg ttgttatttt tcctcttgca aaggttagac actcgggaaa
     4921 gacgggggag atgatgattt tatcataacg gacagaatct ttttcctgct ttatgccgag
     4981 aatttcttca aataaaaact cagtgcttgc gccgaacatc gggtgagaat gtgacgcctc
     5041 accgttccag ttttcccaga gcgtcgtagc tccggaacgc cgcatgaagt cgaaggatac
     5101 atcacccttg ccggcaagca gacgggttgc aagatcgctg ttgccggttt tgtaaagcac
     5161 gttgagaagt atttccgtgc cgaaaatgcc tgtatcaaat tcggtttttt tgctgtattt
     5221 ttttacgatg ttttcaaccg ttctctcgtc gccgagaccg caatcgacgg caaaagcann
     5281 nnnnnnnnnn nnnnnnnnnn nnnnngtatt tttttgtcac ggctcgtgaa tacttttcga
     5341 ttaaacgctc atatttgtta tgccgatttt caagagcttc ggcacaaaaa atcatacgct
     5401 tgagctgaga aataaacatt gtcgtgttga caaaatcctc gggaatttct atcttttccg
     5461 gagtacacca atctccgaga caccagccgt ctttttcttc acgaacgata aggcnnnnnn
     5521 nnnnggccgt tctctgtgcg gctctccata tattcaacaa atttttccat cgacgggaac
     5581 agctcttcaa gctcgtctct atgaccgtaa tggcgataga attgataagg aacgttgatt
     5641 atcgctcctc cccagccgga aggtccgccg ccgccaccgg caaagggcgc ggtgtgctga
     5701 acatgacctg tcgttttgtc ctgacagtcc cgtatatcgg atatccattt tctgtagaat
     5761 tcctctgcgt caaattccgt cattacggcg ttgcaggtta gctgaccgtc acccgtatag
     5821 ccgagccttt cgcggtgcgg acagtccgac ggtactcccg agtgcatatt ggaaagctgg
     5881 gtgttaacaa aagctttgta ataccaatta agtgtttcgt cgctgcactc aaagtccgag
     5941 gtcacatcaa cgtcggaatg aataacgcgt atctcaacgg gctttgcgtt atttgtcagc
     6001 gtaaaatatc tgaatccgaa ccaacagaaa aacgggtgat agattttttc tttatcggta
     6061 atgaaggatt caatctgcct ctgctcatta aacccaacgg acatattgtt aagagtcaaa
     6121 tcgttattta gattttccgc acattccatg actacggttt cgcctgcctt ctcacacatg
     6181 acgacgggat agccggaaac gttgcgttcg attttgtaaa ccgagcagtc gccgaaattt
     6241 ttgacaagct caggcttgag cgtttcaatt actcggtctg cagggcaagc ttgaatnnnn
     6301 nnnnnngttc ttcgccgtcg ggaagagctc cgtcaacagt gttccagttg cggtcaaagt
     6361 ttgtataatc aagcacttct ccgaaaaaaa gattggagct tttaaaaaat cccgaagaaa
     6421 aaacaacatc ttttctgttt gaataaaagc tttcgccgtc aacgtcgagc ttgtagcaga
     6481 gctttattgc gccgtagttc atattgccct cggctttgcg gacggtttga tgataatatc
     6541 ctccgccgac tgccgctgcg agaatatttt ttcctgtatg aagatatttt gtaacatcgt
     6601 atttcatcac ataaatccga tgggtctgct cgtcattgag aggataaaca agatccatgt
     6661 tcgggcgttc gtgataatcg gaatatgccg gaacaaattc gtcggaaccg acacgcttgc
     6721 cgtttatgaa aaaaagaaac cagccgagac cgcagacggt taattcggct ttttggaaat
     6781 cattttttat ttcaatctcg gagcgaaaga gtgccgcgtc gagattcgag cgtgggctga
     6841 gccatctcgc ttctccgaaa acttctctgt gattcataga attctccaat gttgtttcaa
     6901 aaatgccgcc gtatcaaagc atttgcctta atacgacggc agggaaattt aaattttatt
     6961 tcatcgttgc ggtatatctg ctgacatagc ccatgtactg cttctttgca tttgaaccgt
     7021 taaacgcctg gtcgtcgctc ttggtccagc ctgcaattgc gatcgagccg tcgctgagca
     7081 cggcaacatc cattgcgctg tcgtcctgcg caccgttaag acgaaccgtt gcggtcgttt
     7141 taccctcatc gttgagatag
//
LOCUS       DAWBSW010000104         7077 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2115_length_7077_cov_2.396041, whole genome shotgun sequence.
ACCESSION   DAWBSW010000104 DAWBSW010000000
VERSION     DAWBSW010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_7077_cov_2.396041"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2822..2831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3318..3327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1899 a         1709 c         1346 g         2103 t
ORIGIN      
        1 aaagctttga tccagccctt tgcgtcctcc caatccgcaa gatatgcgaa cgcaaagaat
       61 gcaacgaaca ttctgatatt attcctcaat ccccataaat agtaaaacac cgattgataa
      121 ttaaacaaat acccgacgag ggtaatgaag aagaacagac ccacaataac gacgaacggc
      181 attgaataat tgtctatctt cgtgaagcgt tgcgaaaaca gcttcagcaa cagcaaaacc
      241 aagaagccgt cgcataggaa cttgatcgca tcgggtatcg gcaacagctc cgtcaaaaag
      301 gcctgcgcag cggggaagaa aaagatgaat agcatcatcc actccgagac ggttctcttt
      361 tttatatata attttcctgc tgaactctta tccacagaaa atcgttctcc ttaatactaa
      421 tttcgtttta aaaataaaat tattttgcag gcaaaatagc atttgtgctt tagcagtgta
      481 taaaacaggc cgagcctttt attatgaagc tttataacgt cggccgttag gcttttcctg
      541 agcacattgc ttttaaaaat cttgtttaac tcagtctgct tttctttcaa gggtaattcg
      601 gcggcaacga tttgcttcat cgccgttatc gaatacatat gataccttgc ggtaacgtga
      661 tttataacct gtcgcgaaag attgttttgt cgggcaagct cgacgcactt tataagaaaa
      721 acatcgttct gcttttgcct gtccgcctta taggtgcgag tgagcgaatt gctccgctta
      781 taataataat acagactctc ttttataatc gaaacgctca ttatttgaga gaaaagctcc
      841 aaaataaaat aggcgtcctc ggaaataatc tctttttcgg atttaaattt caggtttaat
      901 tctttaattt ttgataaacg gtacatcttt ccgcacgagc ttattccgaa gcccatcgaa
      961 taggtaaaca ttccgggcaa gacctcgttt tgaacctcgt cgctgacaaa aaacgtttta
     1021 tcggttacaa tctctttttc tgaggttctg ccgtcgtcat acacgtcgca cagggcgtaa
     1081 atcacagcgt cgctgtcatt tttttcggct gtcgagacgc atttttcgac cgtttttgta
     1141 tcaacgtagt cgtcgctgtc gaaaaagaat atgtattttc cgcctgccga gtccataccg
     1201 ctgttcctcg ccatgcccag acctgcgttc tttttatgaa ttactttaat tctgccgtcg
     1261 gagcttgccc aattgtcaca aatcaccgca ctgccgtcgg ttgagccgtc gtccaccaga
     1321 ataatctcaa gattatcata tgtttgaccg acaacagact gcacacacct gtccaaatat
     1381 ttttcaacat tatatacagg cacaacaaca gaaactaagg gcatatccgt cataacaaaa
     1441 ctcctacaat atattacgaa aagtcaccga tgcaattatc ccaatcgtat ggaagcacgg
     1501 cttctctcgt tgaacaatgc tcatcctccg ttgcggattt tatgagcttg ttcaatccgt
     1561 tttcgtcagc gccgttcata cggtaaaatc cgggcttatt aataaattcc ttcatctcgc
     1621 cgaagtcggt tgtaataacc ggcaggtcgc acgacgccgc ctccaaacag gaaagcggaa
     1681 catctatgca atgtccgtcc tccgtcacgg ggaaaagata cacgtcgctc atttgatata
     1741 tctcctcaat ttcggggata tacgaatcga atatttttat attggatttt ttgagcagtt
     1801 tttctctcaa ttccgaattc tgttcgtcct tcgtcaatgt gctcactacc aaaagcacct
     1861 gatattcgga atctatattc aacagggtct gaatgttcct gccctcgttc agatgtccta
     1921 catgaagaac cagcggcttt tcgggatcta acccgtattt tagttttaac cgtctttttt
     1981 cgtccgaggt tacaggcacg aattttttcg tatcgacgcc ggtttttatg tatctgattc
     2041 tgcctttggg tacgatgctt ttataaaaat tttcggactg ctttgaaaaa acaatcaggt
     2101 cggttttact cagtcttatt aaaagcttcg atataccttc ggcattgatt ttcatcggca
     2161 gaattacgtc caagccgttt cggcacatta aggacataac aaaaatcctt atcgccgttg
     2221 caatcggctt cgctgggaac ggcacataca agaccttttc ttttttttgc tttattatcg
     2281 aacgaagctc gccgtttaac atgagcttat tcaattccat atgcctgtcc gaaagctcgg
     2341 attttcgctc atatgttata acgaaagcgc cgccatgcga tttttttaat cttttaataa
     2401 gattataggt cacttttaaa cagccttcat caaccttttc ggtcaatgag tttgaaataa
     2461 tcaacatatt aacctcaatc tatctctgca cagcttttgt tctttccgca cccctcgcag
     2521 gtcgaaagag cgtcaagctc ctccttggac accttgccgt cacggaaatc ccgaacgatt
     2581 ttattctcat acatcgaata ctttttcttc ttgaaaaagc gtagaaattt atgcttaata
     2641 acccaagcgg ccggcttata aatatacttg tgcatggcgg gcgaaacaga gcctatcatc
     2701 cagcagttgc ggtcgcagtt tcttacaaag cttctcacct tttccgcctg ctcgctgttc
     2761 caaagctcct cccagctctg cctgttcagg tttcccataa cctgcttttc cttcgtaccg
     2821 tnnnnnnnnn ngcacggcat gacatcgccg taagggtcga taaagaatgt gtcaaagctc
     2881 atatcgcagg gcaacaagcg tttttgagag aaaatataat tgataagtcc gtgattgaaa
     2941 tacgctctga accacttttt gggactgttg gaatcaagca agcgattaac gagatcctcg
     3001 aaattctgcg caaccgtgag gcgatccttg attatattat tagactcaac aaaatagaag
     3061 ctgttgtgaa gcgacgctgt tgcaaactcc ataccgagct cgtcggaaat attgtaaagc
     3121 ggaaccaaat ccgcggcgtt tctgtcctga acggtcatgc cgaagccaac gtccttcatg
     3181 cccatatcaa cgagcttttt gagggttgtg tagcctcggt taaagccgtc gtcaaggcct
     3241 cggatttcat tgttcgtctt ttcaagaccc tcaatagaaa tcctgattcc gacctgcgga
     3301 aattccttgc aaagctcnnn nnnnnnncaa ttcggtcgct ctttttcaaa agctcacgaa
     3361 caatatcttt taaatcgtca cgaataaaag gttcgccgcc cgtgatattg gtgaaataca
     3421 tttcgggaag ctttttaatc gtttcaattg aaatttcttc ctcgggcttc gacgggacct
     3481 tatatcggtt gcacatagtg cattttgcat tacagcggta tgttacaatt accgtgccgt
     3541 ttaatttttt cgacattcaa ttcacctttt aaaattatca tagtaatcct gagcgacctt
     3601 tgaccatgtg aaattgtcga ccgcattttc cctcgccttc tgcttcatat ccttcagata
     3661 gtcggagtca ttcaaaaggc gtgtaatttg agcaacagca tcgtccttta cggcatatat
     3721 gcttccgtcg ccgaaggatg ataaagactc ggaacaaagc actaccggaa ctccggatga
     3781 aattgactca atacacacaa gaccaaagct ctcgtacaaa gaattaaaaa ttgttgtatc
     3841 cgcaatattg tagatctcgt tcatttcctc tccgggactg aatgtgccta aataaacaac
     3901 ctgcttttca acattttctt ccttcgctgt gtttaaaact tcatgatagt attccggcga
     3961 aacaatatct ccggcgaaga caaacacgat attgctgttt ttctttaata aaggcgcaat
     4021 tatttttacg gttctgccct gccctttatt ttcattgacg gagccaacct gtaaaatcac
     4081 ctttttatct ttcaagccga acttttcttt aaccgtgtca attcgctcac tattcagcgg
     4141 agtataaata tcggtattaa caccgttatt gatttgaacg accttattct cttcaatgcc
     4201 aagatttttg acgatattct ttttcatttt aggattcaaa acgaagatta aatcggcgtt
     4261 cctcattccc tcgatttcct gaaaatagcg tgctttcaat gtgctttcgg cttcttcaaa
     4321 cggcaacgac catacgccgt tgtgattggt gtacgctata accgccctgt cccgaagctc
     4381 ctttttaaca agctttaaga agaaaaagat attgtactga ttatgaaaat gaaaaaccgt
     4441 tttttgatcg gtattcttca atattttctt taattttaag gcaagtgcaa cggaataaac
     4501 cactctttta accttatgta taacgcctaa gctgacatcc gatttagaga aaaccgacgg
     4561 caccttaacc tcggttatcg gcaagctgtt cggtgctctg tcctcggcgg aaatatctac
     4621 tgcctcgaca tcaatattca tttgcaaaaa ggccttcgtc agctcctcaa ccacgctttc
     4681 ggttgccgcc gcaaccttgg caggtatcgg tgtgtagcct gtgccgagtt cgtaaatctt
     4741 catgtcattc tccgtaaatt ttaaatagct tttgataata tgtcttaggt gtttcaaatt
     4801 caacagattt gcaattttcg gtgtaataat ccgcatttcg gattacggaa tacagcttat
     4861 cggaaagctg tgttcggttg ccggattcaa acagctcacc cgtttcaccc ggccgaataa
     4921 gctcgggaat gccgccaata tcggcaccga caacaggtgt tccaagcagc agagactcca
     4981 taacggaata cggacaattt tcataccatt cggagggaca aacggtaacg gtcgcatttg
     5041 caatgagcct gttcagctcc tcccccgttt tgaagccgac gtattcggca ttgggcactt
     5101 ttccgatctc ttcaaccgcc ttgccgaagc ctgcgaaaac gaacttaacc tcgggcattt
     5161 gccgagcggc ctcaagcaag gtcaaggtgc ctttatcctt agacagatga ccgaattcca
     5221 aaacatagcc gcccttattt gcggaacata tctcccgctt ttcgataaaa ttatgaatca
     5281 caaccgtttt ctctttaaaa cggggctgtg tatcaagctt gcttttcaaa aaatacgacg
     5341 gacaaataat agtatcgaca taggaatacg ccgtgctcag cttccaaaaa taagcgtccg
     5401 ccgttgccaa aatgctcttc ggcagtgaat tcttcataca ccttgatttg acgcagttca
     5461 tatagttgcc cgtcatgcat ttttcgcaaa catttatatt tgaatcgaac aagccgtggc
     5521 tcggacacac aagctgatag tcatgcgccg tgtaaattat ctttaccttt ttgccgacgg
     5581 tttttctgta tttttcaatc tcaagaataa tgctcggtgt gagataaaac tgaatattgt
     5641 taaggtgaac aacgtcgggc ttaaagcttt ccaaaacctt ttttatctgc cgccttgcct
     5701 gacgtgaata aataatctta aacggtgcca aaaggttttc ggaaatacct gcggtcaaat
     5761 ccatatcggg agcataagca ttcgcactgt ttccgacaat gtttttttcg ttttccagtc
     5821 cgaaatattc gacctcatgg ccgttatcct tcaaggcttc gccgagcttg aaaacatagg
     5881 tttctgcgcc gcccttcggg tacaaaaatt tattaacaag taaaaccttc atatttattc
     5941 accgtaatac agctcgagcg ttctccgagt aacctcgtcc caattaaacc tgttgcagac
     6001 ataatcggac gaaacggatt tgtatttttc aacggcctcg gaatcgttca aaagccgttg
     6061 aagcttttct ttcaggtcgc cgacatcgcc ctttttaaag gtaacgccgc actctccgat
     6121 aacactttta cattcatgaa tatcggaggt gacgcaacag tttccgtagc tcatcgcttc
     6181 aagtaagctc agcggcatcc cctccaaatc ggagggcaaa acatagatat atgcgttcga
     6241 ataaagctcc tccagcaatc cgccctttac aaaatcggtg aaaattatat tgtcgcaggc
     6301 ggattttttc agctcgtcgg catattccgt gctgtcggat gcgccgcccg caatgacaag
     6361 cttcttatcc gtatcaattt ctttaaaggc ttcaatcaga taatgtattc ccttttcggg
     6421 aacaagtctg cctaaatata agatgtagcc gtccttttca aggccgaatc tttcggttat
     6481 ttcctcggcc ggcttcaaag cgtttttact caccccgttg ggtataaaaa cggtttttct
     6541 tgaatatgtg tcctcaaaat acttttgtgt attctcgctc aggacaatga tttcatcggc
     6601 aaattttacc gcacattttt caccggcaaa aatgtaaaac gaggcgaatt taccccattt
     6661 tgcacgttga tggtcaaggc cgtgaacggt tgcgacgcac cttttgccga acaatttcgg
     6721 aatccaaagc atggcgcacg gaccctcggc atgaaaatga acaacgtcga atttgccgaa
     6781 agcggccttc acggcggcaa aaaaggacga ggtcacagcc gccaatccct ttgcatcaac
     6841 cgtaaaaaca cgctttagct ttactccctt atattcggtc atcgcctttt cgtcaaaatc
     6901 gactccgctg acatggtgtc ctcccctgtt aaagcaggta acatcgtgac ccatagcagc
     6961 catacgggta gccaattcgc cgacaacgat ctcgaccccg ccctctctgg acggaattcg
     7021 tttgtgaccc aacatggcaa tatgcaattt ttcgtccttg cgctgaggca tttacac
//
LOCUS       DAWBSW010000105         7063 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2121_length_7063_cov_2.488014, whole genome shotgun sequence.
ACCESSION   DAWBSW010000105 DAWBSW010000000
VERSION     DAWBSW010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_7063_cov_2.488014"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4005..4014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5085..5094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2104 a         1464 c         1718 g         1757 t
ORIGIN      
        1 gaatatgccc gaggagatat tataatagaa aacaaacggc agaccgatta tgtatattaa
       61 aagataaagc tttgaatcgg cgaagatgtt ttcgggcgtg ttgataagct caagtagctt
      121 tgttgagaaa atcaagccga gagccatgag caaaacacac agcacgccgc ctgaaatcag
      181 agtggtataa actgccgttt tcatgtctct gaaacgcttt gcgccgaaaa gctgagaggt
      241 gatgaccgaa cagccgatat tgcagccgaa agcgaacgca atgaagataa gcgtgatttc
      301 atagctgttg ccgaccgccg ccaaagcgtc attgccgatg aatttacccg caacgaagct
      361 gtcggcaaga ttataaagct gctgaaatat tatgctgccg aaaaggggaa ggcagaattt
      421 ccaaaccaca gtatagggct ttcctatcgt taagtcctta ttgataacac acacatcctt
      481 attgacgaat attcttaatt atattataat acaagaaacc aagtatgtaa atcatttttt
      541 gaagcttggc tgacggagtg aggttacaca aactcaaaaa gggagaatga atctatgaag
      601 aaaataattc ttttgataac aaccttttta acagcttttg ttttggtgat tctcggtatt
      661 gtaggaacac tttctgttcg gtcaaagtcc gaaaccgatt cccaatgtac aattgaaaag
      721 atagaaaatt atgaaattaa ggatgcttct tacaatttaa ctaagcttga cggaatgatg
      781 aaaaaatacg agattcaagt aaatggcttg ttgacctttt atttggatgg agagaagatt
      841 gagaataacg aagaacattt ttttcacacg tcgaatgaag aattctttaa taatttaaag
      901 gaacattccg atcaatacgg gctctatgaa gtaaatttta aaattaacaa tgaatcaaac
      961 gaaatgatgt ttgacattga ttggaagctt gatagtgaaa ggcatgccgt catagaaact
     1021 gatttcacag tagaaggaag atcagttgat gcaaaaaagg aagaacagtc cggtctagtt
     1081 gtgctaatag ataaccctga tatgtctgag gaacaggtga aaaatttgct tatttccgat
     1141 ggattgaagc tcacttatca aacggaaagc ggtgcagaca ttcataataa cgaaatacat
     1201 ataaaataaa catgtcttta aaatttgagg aaaatatcgt gaaaagaatt ttttgattgt
     1261 gaggataacc tcagcttgaa agcgggcaca gaaaagaaaa ctgttgttga ttgatattta
     1321 tatttcgccg tgacgctact ctgcaaggaa ggcaagagtg ttcctgcacc gaaagcaatt
     1381 ttattagatt ttcttggtgc gatgtgggta tcgcactcta cggtgatgtg caagaaacga
     1441 acaatttgta ggggtcgatg tccacatcga cccaaaggca gaagaaactc tactcagcgc
     1501 ccttttaggg tagcgaagtg tcgacacggt agtgaatgac agtccggtgg actgtcagag
     1561 ccgtggcgtg accgagccgc agcgagacgc tgtcggcgaa agtgataatg ggctcctcca
     1621 atgggggagc tgtcagcgaa gctgactgag ggagtaaagt ctcatcaaac taaaactccc
     1681 tccgtcacga cttcgtcgtg ccacctccct caagagggcg gccacgaata ttctgcaccg
     1741 aaagcggttt ttatcagatt ttcttggtgc gatgtaggca tcgcacccta cggtaagagt
     1801 agaaaagtaa tgtcgtacag aggcatgaag cagcacaaac ggtaatttta ctctccgaca
     1861 aatgaaataa cacaattcct tgaaaaaagt gtatttttat tcacttatca ttcactgcac
     1921 tgctcgccgc tttttagcaa acaaccgatt tatacgggcg gtaacgtgct attgaagtca
     1981 aacgccttgt aaatttgcat ataatatttt atacacttga aaaaccgttg tgcatatata
     2041 caaaaacgct tgaagtatga aaaaatgtct tgcataaata tacattttat gctaatatat
     2101 gtgcataaaa cacagacaat gctgtatatc ggaggtatta ttatgaataa gaaggatatc
     2161 aaaaaaattg tactcgctta ctcgggcgga cttgatacat caatcatcat tccgtggctc
     2221 aaggagaact acaacaaccc cgagataatc gctgtttcgg gtaacgtagg tcaggcaagc
     2281 gagcttgaag gacttgagga gaaggctctc aagacaggcg catcgaagct ctatgttgag
     2341 gatctcacaa agcagatgct tgacgaggtt gctttcccgt gcgttcaggc aggcgctctt
     2401 tatgagaact atccgctcgg cacagctctt gctcgtccga tcattgcaaa gaggctttgc
     2461 gagatcgccg aaaaagaggg cgcggacgct atctgccacg gctgcacagg taagggcaat
     2521 gaccaggtaa gattcgaggt tgccattaag gctttcgctc ccgacatgac aatcatcgct
     2581 ccgtggagag agtgggatat caagtcacgt gatgaggaaa tcgactacgc agaggcacac
     2641 aatgttcctc ttaagataaa cagagagaca aactactcaa aggacaagaa catttggcat
     2701 ctttctcatg agggtcttga ccttgaggat ccggcaaacg agccgcagta taacaagccc
     2761 ggcttccttg agatgggtat ttctcccgaa caggctccgg acaagccgac ctatgtaaag
     2821 attcactttg aaaagggtgt tccgaccgct atcgacggag ttgagatgga ttccgtagct
     2881 cttgttacaa agctcaacga gcttggcggc gcaaacggca tcggacttct tgatatcgtt
     2941 gaaaacagac ttgtcggcat gaagtcaagg ggcgtatatg agactcctgg cggaacaatc
     3001 ctctacaagg ctcatgagat gctcgaaaca atcactctcg ataaggaaac ttcgcactat
     3061 aaggcacttg taggtcataa gctcggcgag cttgtttaca acgctcagtg gtattcaccg
     3121 cttacagagg ctatccttgc tttcgttaag gagactcaga agacggttac aggcgacgta
     3181 aacgttaagc tctacaaggg caacatcatc aacgccggcg taacatctcc gttctcgctc
     3241 tacgatcccg agatcgcaac cttcgatgag gacgaggttt acaaccaggc tgattcggca
     3301 ggattcatca acctttacgg tctgccgacc aaggtttatt caaagatgaa gaaaaaggcc
     3361 ggtcttgact aatcaataac ggagaattat aagctatgga aaagatgtgg gcaggcaggt
     3421 ttgccaagga actgaataag gaagccgatg atttcaattc gtcgatccat tttgactgca
     3481 agatgtataa gcaggatata accggctcga tgaagcatgc aacaatgctt gcaatgcaga
     3541 aaatcatcac cgacgacgat tgcgacaaga ttcttgccgg acttcagtcg atacttgacg
     3601 accttgacag cggcgctctt aagttcgata tggacgccga ggatattcat atgttcgttg
     3661 agtccgagct gacaaagcgt ataggcgacg tcggcaagaa gctccacacg gcgagaagcc
     3721 gtaacgatca ggttgccgtc gatatccgtc tttatctcag agacgagacg gaagagatta
     3781 ttccgctcat caagaatctg cttgctgcca tactttccgt tgcaaaggat aataaaaaca
     3841 cgattttacc cggatatact catcttcagc gtgctcagcc gataaccttt gctcatcatc
     3901 ttttggctta cgctatgatg tttatccgtg atatcggaag gcttgaggac gttatgaaga
     3961 ggatgaatta ttcgcctctc ggctcctgcg ccctttcggg tacannnnnn nnnncctttc
     4021 ccgtttttcg gaggagatta tcctttggtc gagctgggag tttaagttca ttgagcttga
     4081 cgacagcttc acgacgggct caagcataat gcctcagaag aaaaaccccg atatggcgga
     4141 gcttgtcagg ggcaaaacag gccgtgttta cggcgacctt ttcacaatgc tcaccgttct
     4201 caagggtatt ccgcttgcat acaacaagga tatgcaggag gacaaggagt gcatatttga
     4261 cagcattgct accgttaagc agtgtcttaa agtttttgct ccgatgattg aaacaatgaa
     4321 ggtcatcaag gagaatatgt attctgcggc tcaaaagggc tttatcaacg caaccgacct
     4381 tgccgattac cttgctaaga agggtcttcc gttcagaagc gcatataaac ttgtcggtca
     4441 gatcgttgct cattgcattg ataacggtct tgtgcttgaa acaatgccgc ttgaggatta
     4501 caaaaagtac agcgatttgt ttgaggagga tctttttacc gagatttctc tcgaaacctg
     4561 cgtctccaag agaatttcgg caggcggaac gggtcccgaa tccgttgaaa agcagattgc
     4621 gtatgttgaa aattatattt aaacttaaaa atatttgggc tgtcctgatg aacagccctt
     4681 ttgttctaat taagtatttg aaaatattgc aaaactattt gaaattttta ttaaatgatg
     4741 tataattaca ggagggtgat accgatggtt aatttaaaag gcagagattt tctgaaactg
     4801 cttgatttca caagcgagga aattgagtat ctcgtcgatc ttgcggctga tttgaaagca
     4861 aagaagaaag cgggaatccc acacaggatc cacgagggta agaatgttgc tcttatcttt
     4921 gaaaagacaa gcacaagaac gcgttgcagc tttgaggtcg gtgctaacga tctcggcatg
     4981 ggctcaacct atcttgccga aggttcgcaa ataggcaaaa aggagagcat agccgacacg
     5041 gcgagagttc tctcgggcat gtttgacggc atcgagtacc gcggnnnnnn nnnngatctt
     5101 gttgaggagc ttgcaaagta tgcgagcgtt cccgtatgga acggccttac aaatgaattt
     5161 cacccgactc agattcttgc cgacttcctg actattaagg agcagttcgg tcatcttaag
     5221 ggaatcaact ttgtttacat gggtgacgca aggtacaata tgggcaattc actcatggtc
     5281 ggctgtgcta aaatgggcat caacttcatc gcatgcgcac cgaagaagta ttttcccgac
     5341 catgaccttg ttgacacctg caaggcgatt gcaaaggaaa acggctgtga aattaaattc
     5401 agcgagaatc ccacggaggc cgtaaaggac gcaaacgtaa tttatacgga tgtttgggtt
     5461 tcaatgggcg aaccgatgga ggtttggaaa gaaagaatcg atgaccttat gccgtaccag
     5521 gtcaatcaga agcttatgga tgcggcggca gacaacgttg tgtttatgca ctgtctgccc
     5581 gcttatcatg accataagac aattgtcggc aaggagatcg gcgaaagatt cgactgtgat
     5641 gctcttgagg tcacggacga ggtctttgaa agcgaacgtt caatcgtttt cagagaggca
     5701 gagaacagaa tgcacactat aaaagcagtt atggcggcga cattatgatt aaaaaaattt
     5761 acattgtact tgaagacgga cacatttttg aaggcaagag cttcggcgca gagggcgagg
     5821 ctctcggcga gcttgtcttt acaacaggca tgggcggata tatcgaaaca ctcaccgacc
     5881 ccagctatta cggacagata gttatgcaaa ccttcccgct tatcggcaac tacggcatca
     5941 tggaggagga ctttgaaagc gacaggtcct acgtcagcgg ctacgttgtc agagagtggt
     6001 gcgagctgcc gtccaatttc aggacggagt atgaccttga caccttcctg aaaaagcagg
     6061 gaatagtcgg cgtttacggc gttgacaccc gtgagattac gagaataatc cgtgaaaagg
     6121 gtgttatgaa cgcaaaaatt gtcagctcgg ttgataatat cgactatgac gaaatcaagg
     6181 cttacagaat cgttgacgca gtcaagagcg taagctgtaa ggaaaaatat acggtatcgg
     6241 acgatggagc gaagtataag gttgcgcttc tcgactacgg cgcaaagaaa aacatcgttc
     6301 gtgagcttgt tgcaaggggc tgcgaggtta cggtttttcc gtatgacacc tcggctcagg
     6361 agataatcaa tatgaatccg gacggaatca tgctttcaaa cggtccgggc gacccggagg
     6421 aaaacatcgt tccgattgaa aacataaagg agcttgccgg caaattcccg attttcggaa
     6481 tctgtctcgg tcatcagctc accgctcttg caatgggcgg aaaaacgatg aagctcaaat
     6541 acggtcacag aggcgttaat cagccggtca agcagtttga aacgggcaga acctatattt
     6601 caagccagaa ccacggttac gccgttgttg ccgaaagtct tgacagacga tatgcaagag
     6661 agagctttat caacgcaaac gacggaacct gcgagggtat ggaatacaga agtaagcaga
     6721 ttttcaccgt tcagttccac ccggaggcgt gttcgggtcc gaaggataca aagttcctgt
     6781 tcgacaggtt tatttcaatg atgggaggaa aatacaatgc cgctgaataa agacataaaa
     6841 aaggtgcttg ttatcggttc cggtcccatc gttatcggtc aggcggctga gtttgactac
     6901 gccggagcac aggcctgccg cgttctcaag gacgcaggtc ttgacgttgt gcttgtcaac
     6961 tccaaccccg caaccattat gacggataat gcgctcgccg acgagatcta tcttgaaccg
     7021 ctgacgcagg agaccgtcaa gagaattatc gaaaaagaac atc
//
LOCUS       DAWBSW010000106         6996 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2143_length_6996_cov_2.577006, whole genome shotgun sequence.
ACCESSION   DAWBSW010000106 DAWBSW010000000
VERSION     DAWBSW010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2143_length_6996_cov_2.577006"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1380..1389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2336..2345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3947..3956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4453..4462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2027 a         1560 c         1522 g         1847 t
ORIGIN      
        1 gcgttttctt tgtcccgaag ctgtgctttt gcactgcgag tgggcaaaaa aacgttttct
       61 gcgccgaaag aagccgtttt ttagtctttc ttggatactt ctttgatata ctctctgaag
      121 aaaactacac tttctttgag cgccgagatc gggcaacgct cgttggttgc gtgcgttgta
      181 aggaaaagtt caacggaaac attgaacgga gcataacgaa ggatgttgtc acaaatctct
      241 ccgtaacggt aggaatccgt gccacccatt acaaggtagg gagcaacggc aacatcattg
      301 gggtgaagtc cttcttcaac ctttcttatc gcattgaatg ccctggaatc cgtcggtgaa
      361 aacttagttg cttccttact gaagagacgc tcaacctcaa gatctttata gcgaacagcc
      421 ttgcgaatgt gcttttcaac atcgtcaacc gtatcgcccg ggagaggtct gaaattgacc
      481 gtgatgctcg gtctctgcgg caaaacgttc ggctcgggac ttccctcaca cattgagata
      541 ctctgaatcg ttctgaccat gcttgcagat tcgggaatta ctttaagcac aagcttaata
      601 atcggcttga gaagccatac attgcacatt acaactctgc cgagataggt tgcctttctg
      661 ccgaccgtat caaaaagctc tgtaagaaac ggaagcatat gcgacttaaa ctgatgcctt
      721 tcaacgtcct taattgtatt ggcaagcttc gccataccgc tgtgctttgg agctgccgaa
      781 gtgtgaccgc ccttatcatg aacggtgagc ttcatatccg catagccttt ttcggcaacg
      841 ccgaccgcag caatatatgt atgaataagt cccttgacgt caagacgaag aagtgcgctt
      901 ccctcatcga gaacactgtc aagatgaatt cctcttgaac ggagaacctt aacgatctca
      961 tccgcacccg attccttgga gcaaacgatc tcctcattgt ggccgaagca gagataaacg
     1021 tctctttccg gttggaagcc ctcctccata agggattcaa ccgcctccat aacgcagata
     1081 agatgatttt tcatgtcaag agcgcctctg ccccagatta tttcaccgtc gttatagccg
     1141 ctgaaagcat catgcgtcca gtcgtcctcc gtgccctcgg caacagggac aacatcctga
     1201 tgcgaaagca gcgccatggg ctcaagatcc ggattcttgc ccttccagcg atatataagg
     1261 ctcgcctttg agacattttc cttaaccgta ttctgcttta taagcggata ggcttcatca
     1321 agaaaagcgt gaaatttttc aaactccgac cagtctatct catcgggatt gcttcttgan
     1381 nnnnnnnnna cactgaattg cttttgaaag atgctcggca accttatcca cgtcgatgtc
     1441 ggcatcctcg attttaaccc cttcaaactt tttcggcttg taaaaaattg cccttataag
     1501 agttacaaca ataaaaagag ccaacaaagc caataaaatt ttccaccaca tgattatgca
     1561 atacctccta aaaatacagt gtagatttta gcataatgac aaaaaattgt caatactaca
     1621 tatcgtttta aaagtcaaaa caacagtaaa cggtgtcaag cagtccattg cattacattt
     1681 tcagtgtgag attgtataaa acgtcaaaaa acatactttt tggtacgatt taatgtgttt
     1741 ctattaggta attatgcaca tagcacaatt tcagttgggt aaaagtgcac aaattgagca
     1801 taaattattt gtgcaaaacg cttgacaagt agatactttg gtgctataat gtagtcacaa
     1861 aaagaaaaga aaggaatgaa tgacttatga caaacaacat gtactatgtc gcagaggaaa
     1921 aaatattaaa cgagatcgca aatgaaaaga agttttcatt ccgtgctatc ttaaagctct
     1981 tgacagcagg cgctatttca agaaagtttt aatagcatcg gcttttgcga gccgttaata
     2041 ttgaatttct ctatgcccga tttaaccgcc atgcggattg caccccggta attaccggct
     2101 ccgaaatatc gtaattgcgt gacggcaaag gcagaactca taatcactcg aaaaggtttt
     2161 attcccccct tccttttcgg gtgatttttt attttcattc ggaaacatta ctcaaaaatt
     2221 cataaattgt taacaggcgg aaatattgac aattgttgaa cagtaaacta taatgttgaa
     2281 tgtcgtacaa atataaaagg gagtgaatat tatggaagag acagttcaca acgagnnnnn
     2341 nnnnnaatcc atgacatgca caaacggctg tataatcggt tatcttgccg agaacagcgg
     2401 ccatgatgtt tttcaaaagg acattgaaaa ggccttcggg ataacacgct cgaccgcttc
     2461 aaaggtgata aatcttatgg agcaaaaggg tcttgtggaa aggcagtcgg taagctgtga
     2521 cgcacgtctg aaaaaaatcg tcatgacgga taaatccaag gttgtatcac accttatggc
     2581 tgaggatatg gccctcgttg aaggtattct gaccgatggg ttcagccccg aggaaaagga
     2641 agccttgtat gattatctca aaagaatgaa acaaaatctc aaaaactatc atttaaagga
     2701 aaaggaggaa tgttgatatg catatggtaa aaagacttat gcaatgtatc cgagaataca
     2761 agctgccgtc acttctcagt ccgctgttta tcacgggcga agtcgttatc gagtgcttta
     2821 taccttttat aacggctcag ctcattgaca gcattgaagc agggcgcggc atggacgtaa
     2881 taacacaata cggtatcaag cttgtgattc tcgcaatttg ctcgcttatg tgcggcgcac
     2941 ttgcaggata tttctgcgcc gtagcctcgt gcggatttgc aaaaaatctc cgtcacgatc
     3001 ttttctatgc ggttcagaac tattcatttt caaacattga caaattttcg tcgtcaagcc
     3061 tcgttacaag actcacgacc gatgtaacaa acgttcaaaa ctcatacatg atgataataa
     3121 gaacggcaat aagatgcccg ttgatgcttg ttttctcaat tgttatgaca atcaagctca
     3181 gcccgagact gacaacagca ttcgttgctc ttattccgtt ccttgcaatc ggactcgtgc
     3241 tgatgataaa gatggtaatg ccgcttttca agcgaatatt caagcgttac gatgcgatga
     3301 ataattccgt tcaggaaaat gttgcaagta tcagagcggt caaatcattt gttcgtgagg
     3361 attttgaaaa ccgtaaattc tctgtcgctt ccgacaatgt ttgtaacgat tttactaagg
     3421 ccgagaagct catggcactc accaatcccc ttgtcagcgc cgcaatttac gttatcatgc
     3481 tcatagtatt ctatttcgga gcgaagataa ttgttacatc gggcggcact gagcttcagg
     3541 tcggcggtct ttcgtcaatt ttaacctacg gtatgcaggt tctgatgagc cttctcatgg
     3601 tttccatgac gcttgttatg atttccatgt cattcgcttc ggcgggccgt atcgttgagg
     3661 ttcttgaaga aggcagcacg atcaagaatc cgacgaatgc cgtttgcaaa gttgaggacg
     3721 gatcgattga ctttgaaaat gttgatttcg cttattcaaa aaagcaggag gtcaatactc
     3781 tgcgaaatat aagcttccac atcaactcag gagaaacgat aggcataatc ggcggaacgg
     3841 gaagcagtaa atcaacgctt atccagctca ttccgagact ttacgacgtt acaaaaggaa
     3901 gcgttaaggt cggcggaatt gatgtcagaa aatatgacat caaggcnnnn nnnnnnggca
     3961 gacgaattca tcgagcagat gcccgacaaa tacgacacct atatcgagcg cggcggcgca
     4021 aatgtctccg gtgggcagaa gcagagactt tgcattgcaa gagccctgct caaaaagccg
     4081 aaaattctta ttctcgacga ctccaccagc gccgttgaca caaagacaga cgcaatgata
     4141 agaaaagctt tccgtgaaga aattcccgat acaaccaagc tgatcatcgc tcaacgcatc
     4201 tcctcggttc aggacgccga cagaataatc gtcctcaaca acggtgaggt caacggaatc
     4261 ggcacccacg acgaacttat ggagaacaac gaaatttacc gtgaggtatt tgaatctcag
     4321 acaaaggcag gtgattttga tgagtaatcc gatgccgaaa ggaaaaaaag ctccgatgat
     4381 gggaatgaaa tccggagcaa aaaaaggaac gtttaaaaga cttctgaaaa tgcttttcag
     4441 cgagtacaaa tcnnnnnnnn nngtacaaat ccccgctcat cgcggttgtt gtttgcataa
     4501 ttctttccac gcttgcttca accacttcaa atctgtttct taacaacttc attgttgaga
     4561 tagaaaaagg aataaaaacg gggtgggatg cggcactgcc gaatatcgtc aaaatcattg
     4621 cggcaatggg ctgcatttat cttgtgggcg ttgtagcttc tttcttccat gcaagaataa
     4681 tggcaattgt tacgcagggc tttctcaaca aaacccgtaa gacaatgttc cgtaagatgg
     4741 aaaagctacc gataaaatac ttcgatacac atccgcacgg agatataatg tcctactaca
     4801 cgaacgacat tgacaccctt cgacagcttg tttcgcaggc gatgccggct ctcattcagt
     4861 cggcactgac aattatcatt cttgtagctt acatgcttta tctgagcgtt tggatgaccc
     4921 ttattgtttt cggcgcagta ggactgatga tgatagtcgt taaaattgtc ggcggcaatt
     4981 cggcaaaatt cttcgtccgt cagcagaagg ctctcggcaa aacagagggc ttcatcgagg
     5041 aaatgatgag cggacagaag gtcgtcaagg tctttaacca tgagcaggag agctgcaagg
     5101 attttgacag aataaacggc gagcttttta aagaagcctc aaaggctaac agcttcgcaa
     5161 atattcttat gcctatcatg ggcaatatag gtaatattct ctatgtttgc atcgctttca
     5221 tcggcggact tcttattctc tcgccgaata ctcacaatct ttcattctcg ggtcaggttc
     5281 tcggcattgc cgttgttgtt cctttcctga acatgacaag acagtttacg aacaacatca
     5341 gccagatttc ccagcacatc aacagcatcg ttatggctct tgccggcgcg gacagaatat
     5401 ttgacctcat ggacgaaaag cccgaggttg acgacggcta tgttacactc gtcaatgcaa
     5461 aagagattga cggcaaactc attgagtgta aggaacgcac gggaacatgg gcatggaaac
     5521 acccccacga ggacggaagc gttacctata ccaagctcat gggcgacgtt cgcatggagc
     5581 atatggactt cggctatgac ccgaataaaa ttgttctcca tgatatttcg ctcttcgccg
     5641 aaccgggtca gaaggtcgcc tttgtcggtg caacgggtgc aggcaaaaca actataacaa
     5701 accttatcaa ccgtttctat gacgttgccg acggcaaaat acgttatgac ggaattaaca
     5761 tcaataaaat taaaaaagac gatcttcgtc attcgctcgg aatcgttctc caggaaacca
     5821 atcttttcac cggcacggtt atggaaaata tccgttacgg caagcttgac gcaacagacg
     5881 gtgaatgtat tgcggcggca aagctcgcca atgccgacga attcatccgt atgcttcccg
     5941 acggctacaa caccatgctg acagccaacg gcggaaatct ttcacagggt cagcgccagc
     6001 ttattgcaat cgcgagggcc gcagttgcag atcctcccgt tatgattctc gatgaggcta
     6061 cgtcttcaat tgatacaaga accgaagcaa tcgttcaaaa aggcatggac gctctgatga
     6121 agggcagaac cgtatttgtt attgctcaca ggctctcgac aattcagaat tcagatgtta
     6181 taatggtact tgaacacgga cggataatcg agcgaggcaa ccatgacgat cttatcgccg
     6241 ctcagggcag atattatcag ctttatacgg gctcgtttga taaagaagaa taacgtcctg
     6301 caaatcagca aaaactaacc gtgatttcac tttaagacaa catcgcacaa atgaggagtt
     6361 attatggctt tggtatatgc tgtgctcagc gcacttgtgg attttctcat aacgtatttc
     6421 aaggggtttg ttaaaggctt cggagatatt tggaaaccgc tcttgctgtt tcctttggtt
     6481 tatgtttgct ttttattgct tcatctcggc attttctgcc tttattcttt cacaacggat
     6541 aaggtctaca ccagcaacat tcacaattca taccgaagat tcatgctctc aagcatcaat
     6601 cttttcctta aaaccgcaaa ggttcacgtt catgtcgaag gcaaggagct tgttccgaac
     6661 gacggacagt ttttgctcgt ctgcaatcat atttcaacat ttgacccgat gattacaata
     6721 acggctttgg ctgataaaaa ccttgccttt gttgccaaaa aggagatttt cggtatattt
     6781 gccgcaggaa aatatcttgc aaaatccgga tgtatcggtc ttgacagaga gaataaccgt
     6841 gaggcggtca aggcaataaa ccgagcggcg gacaatatca aaaacggcgt gtgtgcaatg
     6901 ggaatttacc ccgagggcta cgtaaataaa aagccgggca cgttgctccc gttcagaaac
     6961 ggcgcgttta aaattgccaa gaaagcgaaa gtcccg
//
LOCUS       DAWBSW010000107         6981 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2148_length_6981_cov_2.635287, whole genome shotgun sequence.
ACCESSION   DAWBSW010000107 DAWBSW010000000
VERSION     DAWBSW010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_6981_cov_2.635287"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3119..3128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2390 a         1188 c         1531 g         1862 t
ORIGIN      
        1 ctcatccccc ataatcagac agacagacag acagacagac agacagacag acagacagac
       61 agacagacag acgtaattgt ctgcgatttt gtgtatcgaa atataaataa ttgtgctaaa
      121 ccaacctgac agggcttgaa cattaagcct tgacgggttg gttttctata tgaaagacaa
      181 aacttgaagt attctgaaat caaaatacct caagtgatca ctaaaattag gttgaaaatt
      241 ttagcggtta attacctgct ttacaaagtt tatgagaaac aactaaagag aacagaggag
      301 atttttatgg ctgtttttaa gtgtaaaatg tgcggcggca cgatagaatt taatcaaggc
      361 gatacggtcg gagtatgcga ttcgtgcgga acaaagcaga gcttgcccgt cggtcttgat
      421 gacgaaaaga gagccaatct ttacgacaga gctaatcatt ttcgccgcaa taacgaatat
      481 gacaaggcga tgtcgatata tgagcaaatc cttaatgaag acagcaagga tgccgaagca
      541 tattggtcaa tcattctctg ccgttacggt attgaatatg ttgaagaccc cacgactcat
      601 acaagagttc ctaccgtaaa ccgtgttcag ttttcgtccg ttgtttcgga taaggattat
      661 aaatcggctt tgaaatacgg aaccgttgaa cagaaagaaa tttacaaggc ggaagcacgt
      721 aagatagata aaatacagaa gggtattctt gaaatttcat caaaagaaga accttttgat
      781 atattcatct gctacaagga aacggacaac agcggacgta gaacaccaga ttccgtactt
      841 gcaaatgatt tgtaccacca gctcacgcaa gagggctaca aggtgttttt ttcaagaatt
      901 actcttgagg ataagctcgg tcaggagtat gagccataca tatttgcggc actcaacagt
      961 gcaaaggtta tggtcgtgct tggcacaaag cccgaatatt tcaatgctgt atgggtcagg
     1021 aatgaatgga gcagatatct tacacttatc aaaaacggtg aaaagaaaat gctcattcct
     1081 gcatacaagg acatggatcc gtatgatctg cctaaggagt tttcacatct tcaggcacag
     1141 gatatgtcaa agcttggatt tatgcaagat ctcattcgtg gaataaacaa gataatcacg
     1201 aaagatgagc cgaaagaaac aattaaggaa acagtcgttg ttaatgctaa taattcaaat
     1261 gtagtgccgc tgcttgaaag agtatcgatt tttctcgaag acggcaaatg gaacgatgca
     1321 aatatttatt gtgaaaaggt gcttgatatt gaccctaaaa atgcacaggc gtatctcggc
     1381 aagcttatgg cggagctgaa agttaaaagc cgcaagcagt tagccgactg tgcacggccg
     1441 tttgataatc ttgataatta cggcaaggtt attcgattcg gtgacgaaaa gctcgaaaac
     1501 gagattcgtg gatacatatc acacattaaa gaacgcaacg aaaataatcg cctgacagac
     1561 gcctatacca atgcaataaa cgctatgaat tccgctgaaa cagaagctga ttttaaggct
     1621 gccgcacacg cttttcaaag tattgctgat ttcaaggatt caaaagacaa ggcaaaagaa
     1681 tgtcttgaaa aagccgaagt agctcgtaag gatgctattt ttgctgacgg cagagaaaag
     1741 atgtatgttg agagtatttc aagctatgaa ggtgctatca aactgtttga atctgtttcg
     1801 ggttggagag atgctaacaa gcaaatagca gtttgcaagc aaaaaataga acagctcaaa
     1861 ataaaagaag aagaggatag acttgaagcc gagcgcaggg ctgaaaaacg cagaatcgaa
     1921 aaagaaaaaa caaaaaagaa atatattaga attgcaaaaa ttggaggacc gattctggct
     1981 gttgtaatag tttttataat catattgaat acagttatta ttccgaagca agaatacagt
     2041 ctcttagttg cacagtacgg caaagaatcg gcagataagt tagtgaaaat agatgtgggg
     2101 gacacctaca catttggtac atatgagcaa gataataatt tctcaaatgg gaaggaggca
     2161 attgagtgga tagttcttgc taaagatggt aatgagttat tgctgatcag cgacaaagca
     2221 cttgattgtc aaccatataa caaatcatgg ggtgatgtta catgggaaac ctgttctctg
     2281 agaaagtggc tgaatcaaga ttttttggat gtcgcatttt ctgaaagcga aaaagacaaa
     2341 atatcgacag ttgctgttcc tgcaacaaat aatcaaaagt atcacactaa tgcaggtaat
     2401 tcaactcgag acaaagtgtt tatacttaat atagatgaag ctaaaaaata ttttgaaaca
     2461 gatgaaagca ggagatgtgc ccctactgat tatgccgttg cccaaggggc aagtatggat
     2521 aacttttata caacatacgg acaagaagct acaacatgtt ggttgctacg ttcgcccggt
     2581 gaggcacaag aaaaagctac cagtgttact tttgcaggtt caattgcctt tagtggtaat
     2641 tctggtgatg atggtgttcg ccccgcaata tggatttctt tttgaaaaga gagatatttt
     2701 gatttttcca agcaatgcaa cgttttaagt ttaaatttct attataaaaa cggaggaaac
     2761 ttaaatgaaa gatataatat ttctgtttgg gtcgggtgct gattcagatg ttaacagaga
     2821 tttagtgtcc ggaagccaat tcgctgaaag cataattcaa aataaatatg caaaggaaat
     2881 aaataatata acgcaagaag attttaataa ctttaagttg ataagtcata acagcaaaaa
     2941 gatattcata cagacgatag ttcataacga aaatcttgca cgtaaaaagt tatctcgtga
     3001 agaagtaaat ttggttatag attataacaa tggagccaag aattccgata ctactaagaa
     3061 aaaaataaca gaaatgtgca gaaagtggta cgaccttata acaaacacag aaattcctnn
     3121 nnnnnnnnga gggtaatcaa tgcgtattgg actgtattta tggtaatgtt tgaaagcttg
     3181 ttcggagaaa tttcggaatt aaattttgat gccgttctta aaaagctgtc agatgaaaaa
     3241 acagttgcga aaaatatgtt gtcaggaaaa agcaattgct attataacac agtaagcaaa
     3301 gttaagggca actatcatat tgctacaacc aattatacgg ctcttgtgtc cacagccttt
     3361 gaaaatcttg caaattattt gaacggaaaa ttgagctggt ttgaagattt atataatctg
     3421 acggtatatg attgtaccca agaaagcgaa cgtgacttga tagtaaaaca ccccaaaaac
     3481 ataatcccat ttatttttat accgagcgga gtgaaaccta ttgtatgccc taagcaggtt
     3541 aaagaataca ctgatttcaa aagcaattta gaaagcagta gcgttctttg cgttatcggt
     3601 tacaggttca acagcgaaga taatcatatc aattcgttta tagcggactg gttaagaaaa
     3661 gataaaaatg tcctgattta tttcaattat gaagagggct gtaagttgga gaatttgcat
     3721 tggattaaaa tgcttattga aggtggaaaa tataaaacga ttgataaaat tgagggtttt
     3781 aattttgatt ttatagatga gcataaagtt atagacataa aaataaataa gaataatgca
     3841 agaaaaatgg tttcgcattt tgttgagcaa tacaaccaaa gaatagcggc aatgtaatcc
     3901 ataagatagt tgttaaataa gacaggagga aaataaaatg gcgcatattg cagatgtaat
     3961 caaatatgag ggtgacaaca gtacatttat ctggaaacat cctcgggagg actttaacag
     4021 tcttacgcag ctgatcgttc atgaaagcca ggaggcaata ttcttcatga acggacaggc
     4081 tcttgacctg ttcggacccg gcagatatac tctcgaaact cagaatatcc cgaaaatcgg
     4141 cggagcatta aagcacaaca caggattaaa aaatcctttc cattgcgagg tctatttcat
     4201 taatcagact gttcaaatgg gattaaaatg gggaaccgat tccaagataa gatacataga
     4261 ccccgaatcg ggcgtgcccc ttgaattggg tgcaagcggc gagatgaatt tgcaggtttg
     4321 cgatagcagg aagctcctgg ttaagcttgt cggcacgatg aaaggaatag catgggatgc
     4381 cgacagcaag gattttacaa aatctattca aaattctttc agaccgatga tttccacaga
     4441 ggttaaagcg aaccttgcga ctgtaattaa atcaaaaaaa ataaatattc tcgaagttga
     4501 cgaacacctt gttgaaatag gtaacgagct tcaccaaaga attcttccgg gctttgaaga
     4561 gtttggcctt acaattccac agttctatct caatacggtg gttctgcctg aagaagatcc
     4621 gaatttcaga aagataagag agctgtatac aatctctttg cagaagcgaa tgattgatgc
     4681 cgaaaaggat atcagaacat caaaagcgca ggctgaggcg gaaattaccg ccgctcaaag
     4741 agctgttgag attgaaaagc aggcgactca aaccgaaata gcacgccacg aagcggaaag
     4801 aaaaataatt gagtctgaag cagaggcaga ggcgatgcgc cgtgcaggtt acgccgaagc
     4861 acaggttatg caagctcagg gaatcaccaa gaaagactat gtacaggcag atgttcaaaa
     4921 ggcttttgcc gaaggcatgg gaaatatggg aggctccgga ggctcggcaa tgggagacat
     4981 aatgggactc ggtatcggat tgcaggcggc acaagccatg agcggccaaa tcggaaatat
     5041 gtttggcacg tttaataatc aacagagccc acttaagtgt gcaaaatgcg gagcagaaat
     5101 tccgcaagga tcaaaattct gcttgcagtg cggcgaaaag gttgaagcaa actcaaatga
     5161 tacggtcgtt tgtccggaat gcggaaaaac cgttgccaaa ggcaagttct gtttggagtg
     5221 cggacataag tttgtaacta cttgtccgaa ttgcggcaag gaaattcctg aaggtgcaaa
     5281 attctgcctt gagtgcggaa ctaaaattta aaatgagggg tatataatca tgaaaatcaa
     5341 aagacttgct attacaattt tcacagtatt gtttgttgcg tttacgcttg tctcgttctt
     5401 tgcatacgat aaatcatctt ctgtcccaat tgtttgtgaa gataaatcct atgctcagaa
     5461 atatgcaaag catcataata tcgactataa aattttgtct gattccgata agaatatcgg
     5521 tcttgtaaat cttgacgatt ttaagtataa cgacgacgga acattggttt cctacaacgg
     5581 agacagtgaa acaattgcta tccccgaatc gatcaatgat actgctatca tcagggttaa
     5641 agaaaacgca tttgatgatg caaaaaattt gaaggagatt tatatttcaa aaacactcct
     5701 tatttttgaa ccgaacagcc ttaagggggt tacggtttat gtcccggagg attctcttgt
     5761 ttataatctt atgcaatgga aggataagaa taaaattgat ctctccgtcg gagaaaatac
     5821 aggcgatgtt gttgtttcaa attttgatga ttttaaggtc tcgtcaaacg gcacgttgga
     5881 atcatatgta ggtgacgatt caattatctc cattcctgcc aaaatcggtg acttgaaaat
     5941 aaggaagatt gccaaggatg cttttataac cgcaaagcgt ttaacaaagg tttatcttcc
     6001 cgaaagcctt gaaaaatttg aaccgatgcg tgttgaaaac gttgaattct gtatttcaac
     6061 aaagtccgat gcttataaag atgcgaagga taacaagtca aaggttgatt tctcaagcaa
     6121 tacggatcat agcaaaatgg cggcttctct cgatgaggac tttgactata acgaaaacgg
     6181 ccaaattctt gaatacagag gaactgatca aaccgttgta attcccgaag agatcaacgg
     6241 aactaagatt atttccgtta acgaaaaggc atttgacaag gcaaaagaca caaagacaat
     6301 ttatttgccg gaaacaatga tagctttttg gccggaaact cttgacggag taaaggttta
     6361 tgctccgagc gacgctccca tcaacaacat ctacgccgat aaaaagtttg attatgttac
     6421 atatgcggat tcatattatg tgaactttta ttcggcgaac attccctact cgtatgatga
     6481 tatttcaaat gacgaagtga gggtaaacag ctataacggc gaggacaaaa atatcattat
     6541 tccggaaacg attgacggca aaaaagtgac agctatcagc gtggacgcac tcggtgtcgg
     6601 agcagaaagc attttgattc ccggtacagt cgataaaatt gaaacaaagc tttatactgc
     6661 aaaatatgat gcggcctttt atattggttt ggtaatcgca ttactgggat ttgctttggc
     6721 tttggttgct gtattgaaac ttaatcttga cgaaagcaag gaaaaaacat tcctccgttt
     6781 aagctttctt cgtacagcct atactgtttc aattttgtcc gtaattcttg cggctgcata
     6841 tatgtttgta cttccgagat ttatagtcac tcccgtatgg gttggcggtg tgttcgcagt
     6901 attgattgtt gcgttcggaa caacatcttt tataaagacg aatacggcaa cagagcttgt
     6961 tgaaaaggtt gataaggagg t
//
LOCUS       DAWBSW010000108         6959 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2155_length_6959_cov_3.401217, whole genome shotgun sequence.
ACCESSION   DAWBSW010000108 DAWBSW010000000
VERSION     DAWBSW010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2155_length_6959_cov_3.401217"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1901 a         1647 c         1561 g         1850 t
ORIGIN      
        1 ccgagaagct ttcccttatt ttctcgccga gttccttgag ccttacaacg tctttttcgt
       61 caaaagtgcc gtcgggcttc ggcggaatat tcaaaataaa agttgtgttg ccaccgaccg
      121 aacgcaggta tgtgtcaaac aaacggtcga gcgaataagg ctcttcgttt tcatggtaaa
      181 accaacccgg gcggatagac atgtccacct cggacggagc aaaaaccaag cccctcgaat
      241 atataatatt gctgagcgaa ccgatatttt cgtccatgtt atacatgtaa gaaaggtcgc
      301 cggcaaaagg acccggctcg gtttgaattt cgcaattcgg gcaaagctca gacggaacga
      361 cagcccattc ggcatgtctc gacgaaccgt cctcgttgcc gcaccagcga acgggacctc
      421 tgtcgttaaa aacagttgca ttcggctgat atttgtgtat cagctcaaag taaccgtcaa
      481 aatcgtaaac ctgcttcttt ccgttcggcc cctcgccgca ggctccgtca aaccaaacat
      541 ggaatatttc tccgtagttt gtcagaagct cggtaagctg cgccttgtaa taaacattat
      601 attcatccgt gccgtaaagc tcggaatgtc tgtcccatgg agaaaggtaa aacccgaatt
      661 tcaatccgtg gcggcggcat gcttcggaaa gctcctttac aatgtcgccc ttgccgttcc
      721 tgtacgggct ttttttcatg ctgtgatcgg tgtattttgt ctgccaaagg caaaatccgt
      781 cgtgatgctt tgccgtaatg acaacgccct tcatacctgc ggattttata gccttgcacc
      841 aaccgtcaca gtcaaggtca acgggattaa aaatcgacgg atcctcgtca cccgtgcccc
      901 attcgagatt tgtatatgtg ttcggactga aatggacgaa agcataaaag ggcgtttcgg
      961 tcaaaagcct gagctgattt gcgctcggct taacatgtgc cgcctgcttt atgaagtcct
     1021 tcatttctgt cctccgctct tcaaatttta ttccacaaaa tatagtcgtc aatcagaacc
     1081 gctctgacgg gtgcgccgtc gattgcgccc atttttacgg gcaaagcaat aagataataa
     1141 tcgcccggct caaccctcga aaggtcgaga ttttcaagta tcgcaactcc gttttgcaga
     1201 aaagccttgt gtggcttgat ttgattgcct gatttgccga ccgagtctgc gtccgtgccg
     1261 ataaggcaaa tacccgtatc gaccgcttca taagcggcgc tttccataaa gaacgactgt
     1321 gcatcgccct taatgagcag acgggaacac tttctcggaa aatatctgtt gacatattcg
     1381 cctgttataa ctcccggcgg aacttcaatg accgtacact gaccgatata tttattgagg
     1441 tcaacgtcct cgatagtctc gccgccgtca aggaaatgac agggcgcatc ggcatgcgtt
     1501 gccgtatgaa cacacgcata aaccgccgaa aggttacact cgtcgccctt ttcaatggaa
     1561 cggacggtat caagccttgt ttcggggtcg cccgggtatt caactgtggt caaaagcggt
     1621 tttgaaatgt caatgatatt catagtaaca tcccttatta tttgttatcc aaagatgcct
     1681 gtttaagata tgcgttgatg aagccgtcaa ggtcaccgtc cataacggcg ttgatgttgc
     1741 cgacctcgta gcctgttctg tgatccttaa cgagagtata tggcatgaaa acataggagc
     1801 gaatctggct gccccagccg atttccttct gctcgccctt gatgtcctcg atcttatcga
     1861 ggtgctctct ctccttgatt tcaagaagtt ttgacttaag cattctcatt gcaacctcac
     1921 ggttctgatg ctggcttctt tcaacctgac atgaaacaac aatgtttgtc ggaatatgga
     1981 taagacgaac agcagatgag gtcttgttaa ccttttgtcc gcctgcgccc gaagctctgt
     2041 aaacttccat tctgatatcc tcgggattga tctcaacctc gatcgtatcg tcgatttcgg
     2101 gcattacttc aagagaagcg aacgacgtat ggcggcgtcc ggatgaatcg aacggtgaaa
     2161 cacgaacgag acggtgaaca cctgcctcac ttttcatata gccgtaagca ttttcgccct
     2221 caatgcagag aacggcagat ttaagacccg cttcgtctcc gtcaagaaag tccagcatgc
     2281 tgaccttaaa gccgtgctgc tcaccccaac gggtatacat tctgacaagc atctggttcc
     2341 aatcctgagc ctccgttccg cctgcgcccg cgtggaaggt gagaatggca tttttgccgt
     2401 catactcccc cgagagcaga gtgctgaggg tctgagcgtc aagctccttg ataaatgcgt
     2461 caacactgcg ctgacattcc tcaagcatat cgagatcctc ttcctcgtcg gaaagctcga
     2521 tcatggtcag atcgtcctca taggtcgctt taagattttc atatttttga attttgtttt
     2581 taagtgatgc aattttctgg agaacgactt gcgttccggc aacatcgtcc caaaatccgt
     2641 tttctgctgt ctttgcttca agctcggcaa cggtcttttt catttcttcc agaccgagag
     2701 cctcaccgag ctccttgagc ttatcctcat actccagcaa atgcagtctt aattcttcaa
     2761 attgtaccat tatattacct cacaatacca tatacacata gttttactaa tagattatat
     2821 cgtaaaaaag cccgtatgta aagaaagatt taaataatat ttcctaaaaa gttcaaaaaa
     2881 aagctacaat atgcttgact cttgtgttat aataataccg ttaaaaatga aaaatggggt
     2941 aaattgatga attatacagg aaaaatatgc gacggatgcc gagaaccgtt caaagacggc
     3001 gatgacattg ttgtttgtcc gatatgcgga actcctcagc accgaagctg ttacgaaaaa
     3061 aatcataaat gtgtcaacga atatctccac tcaaccgact tcacctggac cgatcccgat
     3121 gcggagaagc aaaaaacgga agctgagcag gctgtccgag aggaacagtc tcagcccgag
     3181 caacagccgt ttgttcaggc aacgccgcag agcatggagt ctgttttcat gcgcggagtg
     3241 ctttacgacc ctaaggacga tatcggcgga gcgaccgtcg gcgaagcagc cgattacatt
     3301 cagaattccg ctccgagata tatttccaag tttatgaagc agaaaaagaa aaacggcaaa
     3361 atctcgtgga attgggcggc atttttcttt gcgccatact ggttctttta cagaaagctt
     3421 tacaaggcag gcaccgtttt ccttgcgctc tcggttgcac tgagccttgc aacgatctca
     3481 cagaccgaaa aggtgtacgg cgtttatgaa aaaatggtct ccgttcagca agagataaat
     3541 gaacttgtca aaggtaagga taatctctcg ggcgaggaca gtcaaaaatt tgataaactc
     3601 tatgcagaag cgcaaaaact aaccaaggcg gcgatcccga cgctcgctat tatattattg
     3661 attcaaaaaa tcattcccaa tacaattgct gcacttatcg caaactattt gttaaagaaa
     3721 aaaatgctca aggtcatcag ccttgcacac gatgcatcga ctgagccgaa cactgttaag
     3781 tatacaatta tgcgtaacgg cggagttgcg attattatgc cgataatcgc aattatggtg
     3841 gataattatc tgccaagcct gctgttgaat attgcatata aaataaattt ctgatttcag
     3901 gaggactttt tatgaaaatc aagaaagcta taatccccgc agcgggtctc ggaacaagag
     3961 ttctgcccgc atcaaaggct gttccgaagg agatgctcaa cattgttgac aagcctgcga
     4021 tacagtacat cgttgaggaa gccgcagcgg caggtattga ggatatcctc ataatcacaa
     4081 acagaggcaa gggtgttatt gaggatcatt tcgatcacag ctacgagctt gaggataagc
     4141 tcgcaaagaa cccgagcaaa aagaagctct atgaggacgt tctccactgc tcaaacattg
     4201 ctaacgttta tttccttcgt cagaaggaga caaagggtct cggccatgct atcatgtgtg
     4261 caaagagctt cgttggcaac gagccgttcg caattctcta cggtgacgat gttatcatca
     4321 acaacgaata ccctgtaacc aagcagcttt gcgatgtttt tgaagcaacg ggcaagggcg
     4381 ttgtcggcgt taaagaggtt ccgagaaaag acgttccgaa gtattgttcg cttgatgtca
     4441 cacaccatga ggacaacccg aaggttatgg acgttcacaa catcatcgag aagccgaccg
     4501 acgaacagat catgtcctgc tattcgattc tcggccgtgt tgttgctccg ccgcaggtat
     4561 ttgattacct tgcagaagat cttgcaaaaa ctcctgaggg agaggagctt tatttcacgg
     4621 atacgcttac ccgtctcggc aaagaaggcg accttcttgc gcttgacttt gacggaatca
     4681 gatatgatat gggcaacaag ctcggtatca tgaaggctaa ctgtgaggtt gctctcaacc
     4741 atgatgaaat cggtgaggat tttaaggcat atatcaagga tcttgcagca aagctttaat
     4801 aaacataata aaattaaggc gttcactctc tcctgagggc gaacgccttt ttgatttata
     4861 tttataatgt aatgcctgcc tctttaagcg aattggcaac ctgaatatcg gggcgcaaat
     4921 aattgagata ataatcacgg ttgcggcgct tcatttcata aaccgaatcc ggatcgttga
     4981 atagcttttc aacatttgcc atacattcgt caacactttc aaaatcgaga tagttcttct
     5041 ctgcctcaaa gtctcccgtc acctcataac gaagcttttc gtttatgatt gcacgggaag
     5101 cggcaacgta ttcgcccgtt ttccacccga ttgagccgtg caggcctata ctgccgatac
     5161 agatatccga cttttttatt gtttcaagaa aattttcgcg tttggtaaga ctgcccgaca
     5221 aaacaagctc ggggcaaagc tcccttgcaa gctcgccgtc ataaattccc gttaccgaat
     5281 ttttaggata ttcttttctc aatgctttcg ttatttcaat tcgcattgaa ttgacggcat
     5341 caagattttt ttcggaagtc tgcgaagcac tccaaagacg tgtcataaac aaaattctct
     5401 gctcatcatt tttgttcggg acagcttcaa agcgatcata tgtgaaatat ttcagcggtt
     5461 ttcttcccct gaaaaaattt acggcttcaa gcatcctttt ttctggattg tgattgaaat
     5521 aaccgtttcc gtcgcaggtc gcaagatagt tgaagcccca cctgtaaatt ttgccgctat
     5581 gcttcggaaa aagcttgtcg ttcagctcgt cggaaaagct gcgtttgaaa taaaaatcgc
     5641 aggagtcaat atatctttca atctcggcag gatcacggaa attgtaaccg tccgccatgt
     5701 cataagcaag acgttttccg tcgattatcg cttccattat gctcgccgag ggcattttga
     5761 cattcgtatt cagcttcagt ctgagcttgc cctgcttttc aagcaaaata aaccccgtca
     5821 gcaccgagca taggtgcggc gttcttgcgg gaatattcag cgtaacatct gtcatacaaa
     5881 aacctccaac cgcagttgtt gtttattata acatattttt aggagctcaa caagatatga
     5941 aagaatgcaa atatacatca aacacaaaat actcccaccc gtcggacgag tgggagtaaa
     6001 tttatatttt tatcttagct tggcttatgc cttcttgcct ttctttgaaa ggattacatt
     6061 tgtgataata ccgaggataa gtgcgcaagc gatagaagtg agcgttacct tgccgaagtt
     6121 tacggtaagt ccgccgatgc cgcagataag gataaccgct acaacaaaga ggttcttgtt
     6181 gtcgtcaagg tcaaccttct ggatcatctt aagtccgctg actgcgatga agccatagag
     6241 agtgatgcaa acaccgccca ttacgcagtt cggaatactt gcaaggaatg ttacgaacgg
     6301 tccgaagaag gagatgatca tgcacatgat tgctgtggcg aggattgtta caacagaagc
     6361 gttaccggtg attgctacgc agccgaccga ctcgccgtat gttgtgttcg ggcagccgcc
     6421 gaagattgcg ccgaacattg aaccgacgcc gtcaccgaga agcgttctgt ggagacccgg
     6481 ctcctcaagg agatcctgct caatgattga agaaaggttc ttatggtctg cgatatgctc
     6541 tgcgaataca acgaatgcga cgggaacata agcaactgca acagttgcga gatactgagc
     6601 gtcgatttcc ttagcgccct ttgcagcctc aagaaatgtg aaatcgggaa cggagaacaa
     6661 cttaaggtcg tggaaaaccg tgaagtcgat aacctgaagt gctgtgttgt ctgtcttgat
     6721 accgattacg gtgaagattg cagcggcaac atagcctgca aggataccga taatgaacgg
     6781 aatgagcttt gtcatcttct tgccgtaaac cgagcagata acaacagtaa agagagttat
     6841 gagtccgcag ataagcgcaa catacgggct tgcggttgat acggaggaaa caacgtcaac
     6901 gatctttccg ttttcaaccg tctgagtggt aacctcggtc ataacctaac cgagtgtga
//
LOCUS       DAWBSW010000109         6903 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2169_length_6903_cov_2.639895, whole genome shotgun sequence.
ACCESSION   DAWBSW010000109 DAWBSW010000000
VERSION     DAWBSW010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2169_length_6903_cov_2.639895"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3583..3592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1579 a         1822 c         1467 g         2025 t
ORIGIN      
        1 aaattcggtt ccagcccgaa aatatgcgac tcctcgccgt tcgtgttaaa cggttgttta
       61 ccgttcggga aacgagaaca ctgaacctga tttgttaact gatcatactg atgagtccac
      121 atatcggggc tgacagtcgc agggaaagca ttgaaagcgg tcttttccaa gcgctcagac
      181 cagtagctgt cccctgtcag cgcaataagc cattcatatg aatacatcgc ttcggcaacc
      241 gaacagcact ccgagccgtg aatgggactt gttccgctca aacattcgtc gcccgaaaaa
      301 tgtccgcatg ccatgccatg gtcacgcaaa agaatttcca tcgcctgctt tgcaaattcg
      361 ttaggatcac caccgatgaa ttcggtcatc agtgcttcac ccttgagcat cattgcaatg
      421 ttgacaacat gatttagata gctccaattt tcacgtcttt cggcatagcg ccatgattta
      481 aacatttttt tatagtcaat gccctgtatg tagagcttgt caacaaggtc aagctgccaa
      541 tcctcaggct ttctttcgcg aagccaaaga atcggtataa gacactcgaa ccagcgagcc
      601 gacccccaat caaaaagagt tcggctgttc atgtgtgaac tgagattttg aagcgcccgt
      661 ctgacggcgt tttcgattcg ctcatcgccc gagcaatctg catatacgac aagtgccttg
      721 cagattataa agcaagccca gatatcatag ttaacacgct tttccttttc gcacgggcag
      781 atccagccat cctcacactg acgttcaaga atagcgtcaa catatttctt cgcacgagcc
      841 ttcatgtcgt cattgtcaag aaggtaagcg agcggcacaa aaccgtcgag ccaataaggg
      901 actctctccc agccctcgtc atttccgcct atccagcttg attccttgac actgttccac
      961 atcttatcaa gattgccgga aagtcctttc gcctgaattt cgagctgatt tctgagccaa
     1021 cccgtggcct ttatttcatt ggtggtaaat tgtttgaaaa tcatagaccg ctccttaact
     1081 tattccgagc ttcttagcca tatccgccgt ctgaagctcg agcatctttg aaacgccctc
     1141 ctcttgcatc gtgatgccgt agagaacgtc ggcttcttcc attgtgcctc gtctgtgggt
     1201 gatgagaata aactgcgtat ttgtcgtcat gcggcgaaca tagcgtgcat aacgggcaac
     1261 gttgacatca tcaggcgcag cctccacctc gtcaaaaata cagaacgggg acggggcaac
     1321 cttgagaata gagaagagaa gcgcaatagc cgccaatccc ttttctccgc ccgaaagcag
     1381 gtcaatattt tgaacattct tgcccggcgg ctgaaccttt atttcaatgt tacattcaag
     1441 cacgtcgttc ggattttcaa gaataagctc tgccttgccg ccgccgaaaa gctcaacaaa
     1501 agtttcgccg aaatagctgt tgatacgtac aaactgagcc ttgaactgct ccgccatttt
     1561 gctcgtcagc tccgtaatga gtctgttcag ctcctccctt gatttctcaa tgtcctcaag
     1621 ctgagcgctc ataaattcgt aacgctcgga aacctcttta tattcctcga ttgcaccgac
     1681 gtttacattg ccgagggcac gaatcttgcc cttaatctcc tgcaattttc tgtgcgcctg
     1741 ctgataatca tcaattacaa tttcaagcgc ttcggcctct gatttcgtca gttggtactc
     1801 gtcaaagagc ttatttatcg cattttcaag ctgctgctcc atctgagcct tgcgttcctc
     1861 aagacgagcc agctcaccgc tgattttttc gcgctcggcg gattttgaac gctcctgcga
     1921 acggagctgt gcgcttcttg cttcacactt tgtgcgctcg gtaataagcg tttcaatgtc
     1981 actttttgcg cttgcgccaa gctcgtgaag ctcctgcgcc tgctttgaaa gctcttgaat
     2041 ttttatctca atttctttat ttgcgttttc aattaccgcg atttcatcgt ttaggtcgtc
     2101 cagcctgcct tcgtggcttg ccatacggcg attgagaaga tcaacggctt cctttttcgc
     2161 ctcaatatcc ttttcgagag cgagaatatc gagattgatt cttgcctcgg tctgtgcaag
     2221 ctcctccttc ttttcttcaa gcttctctct gccgtgggta acgacgtcaa gatcagcctg
     2281 tagggaaccc atttccttgt tgagcttgtc aatctcggtc tgagcttcgt ttttcaatgc
     2341 ttcaagatca ctgattctca gcgatgccga cttcttctca cggcggagct cttcaagagc
     2401 cgcattagcc gtgtcaagct taccctcgat aagcgcaagc tcgctctcct tacggataac
     2461 gtcctcctgc gtgcgcttga ggtcagcctc ggaagcgtcg agatcagcct tgaccgacga
     2521 aagctcaacg ctcaattttt tatacgcttc gttatcctgc tcctgctcgg caacgagctt
     2581 tgcaatctgt gaattgagct tgtccttctc attgcttcgg ctgaggatac ctgcgttgtg
     2641 tcctctcgaa ccgcccgtca tcgagccgcc ggcattgaca acctggccgt cgagagtaac
     2701 tattttaaat ctgtatgaat atttcttcgc catggtaatt gcgctgtcga gatcctcggc
     2761 aacagcggtt ctgcccaaaa gcgactgaat aatttcatta tactttccgt catagtcgag
     2821 cagcttatcc gccatatcaa caaatccgta gcaatcgtca agtccctttt ccgtaaacgg
     2881 ctttgactta actgaggtca gcggcaggaa tgttgcacgt ccgcccttct tctctttaag
     2941 gaagttaatg gcacgctttg catcattttc gctgtcggtt acgatgttct gtatcgccgc
     3001 accgagagcc gtttcaaccg ccacggcata gcgatcctca acgctgatta gctgggagag
     3061 cgggccgtga attcccctga gtgctctgcc ctttgcctca cggacaacgg ctttaacagc
     3121 acccgaatag ccctccatgt tcttttccat atcgtcaagc aagcgaacct tgtccttctt
     3181 ctggagaata tcaagtcctc ttgcgtcgat tgccgatttc agcttgtccg ccttttcctg
     3241 acgggactga acccgaagat tatatcccga aacggcattc attatctctg tcgccgtgtc
     3301 attaagcttg tcaagctgag cctgacaagc gttgcgcttt tcttcaagct cggttatgta
     3361 gccgtttctg gttgagagca ctccctcaat gttgctgaca cgtcctttga tttcatcaat
     3421 agatgaattt gccgtggttt cctcaattct cttttcaccg accttttgag taagagcggt
     3481 tattttttgc gaaagctcgg cgattcggtc tgaaaactcg cctgtctcgt ttttcgtatc
     3541 ggctatgctt gctgccgcag agtcaagctc cttgcgtttc ttnnnnnnnn nngcgtttct
     3601 ttttaatctc gcctaaaagc tcattgattt ccgcttcggc ttcttcaacc tcttttttga
     3661 gggagttttc gctgtttgaa gcgtcgctca tatcacgctt tatacgatcg atcgtctgtt
     3721 cgttatggag aattgtgttt ttctcaacgg agatctgacc ttcaagctga acagcttttt
     3781 cctcatagct tgtcgaattg tttcttatat tgtcaatttt gaccgtgatt tcctgaccct
     3841 tggcgattat ctcctcggtc tcgttttcaa tctgagagag tgaagcttca attgaatcat
     3901 actgaccctg agcgatggag agcttatcct cctgctctct caatttgacc tttgacgtat
     3961 caatggtatg aagccaaagt ccgatttcaa gctccttttt ctcctttgca agaacgagaa
     4021 acttctgtgc cttttcactc tgcgttttaa gcggtccgac acggctttca agctcggaaa
     4081 gaatatccct caagcgaaca agattttcct ccgcctgatc gagttttttg ttagcgtcgt
     4141 tgcggcgata tctgtaatgg gaaattcccg ccgcttcctc aagcatatca cggcgctgac
     4201 ccgactttga agaaatcaaa tcggcaattc tgccctgact gacgagagaa taaccgtcac
     4261 gtccaaggcc tgtatccatg aaaagctcat ggatatcgcg caggcggaca acctgaccgt
     4321 tgagcatata ttcactctct ccggaacggt aatagcgacg tgttacggaa acctcgtcct
     4381 tatcgttgtt aagagttctg tctgtgttgt caagacgcag agtgacctcg gcaaagccga
     4441 gaggttttct gaccttcgtg ccgccgaaga taacatcttc catttttgag cctcgcaggt
     4501 tcttagttga ctgttcaccg agaacccagc gcacggcatc ggcgatgttg ctcttgcccg
     4561 agccgttggg accgacaaca gccgtcattc cgtgattgaa attaaatacc gttttgtcgg
     4621 ggaaggactt gaagccctgc atttcgagcg cttttaatct cataattctt cgttccttat
     4681 tatgtaaagt tgatacttgc ctgttttgtt gtccgccgta attttagcat cgtagcccat
     4741 ttctttgagc tttaaaatat tacagcgttt ctgccccgtc atttttgaaa ttgcgtcgcg
     4801 ggcaacataa atagtaattt ttccttgagg aattttattt tcttttatat attctatagc
     4861 tttgtttata tagatttttc cctcgcaaag ctctttgaat gccggatggt atgcgcccgc
     4921 aaggtactcg ccctcaacgc cgccgcccga atgaagtccc gttctgataa cggttatatc
     4981 cgcctcattg aacatcaaaa gcagctttgc acaaagctct gccgccgaat caaccgtttc
     5041 gggctgatat ttgccgcttt tgtaaagctc cgcaagctcg gtattctcaa gcacaacggt
     5101 cggataaatg cgaaccgtcg ccggtttcag tgcaataatt ttcttcgccg tttcaatact
     5161 gtcctcatct gttgagccgt aaagccccgt catcatttga agaccaagct cgaagccgta
     5221 gcttttcagc atttccgaag cattgataac gtcctgagag gtgtggcctc tgcgattaag
     5281 tctcagaacc tcatcgctca tgctctgtgc tccaagttcg actgccgtaa ctccgtagag
     5341 ctttaaaatc cgacagattt cgtcatctat cgcatcggga cgtgttgagc atcgaatgcc
     5401 cttgaagctg ccgttttcga catatttata cgccgatttc aaaagtccga ccataacatc
     5461 acggtcaata gccgtgaagc tgccgccgaa aaaagctatt tcgccgcctc tgctgtccgg
     5521 attgttcatt gcaattttaa ccgcctcgtc aacgtcggaa ggcgttacct gcttgctctg
     5581 acccgagatt gcacgctgat tgcaaaatgt acattggtga gggcagccgt tgtgcggaac
     5641 aaaaagcccc acgtttacat gcttcacagt cccataagct ccaaggcttc ccttgcagcc
     5701 tcctgttcgg cgcgcttttt gcttctgccg actcccctgc cgattacgtt tgaattgaga
     5761 tggacctcaa cctcaaagcg tttgtcgtgg tcgggaccgc tctcacctgc aaggacatat
     5821 tcaactacct cgcccgggtt gcgctgaaca acctcttgaa gcatggtttt gtaatccttg
     5881 acggaacggt gctttcttat agcttcgtca atatatttaa gtacaaattt tttcgcttcg
     5941 tccatgccgc cgtcaagata gattgcggca ataacagcct caaatgcgtc cgcctgaatc
     6001 gaaggacgct ctctgccgcc tgttacgacc tcgcccttgc cgagcatgag gtacttgccg
     6061 agttgaatct cacgtgcaaa tgacgcaagc gacttttcgc agaccattgc cgcacggagc
     6121 tttgttaaat ctccctcggg aaaatccgta tagttttgaa aaaagttttc cgccgttata
     6181 aagccgagaa cagaatcgcc gagaaactcg agacgttcat ttgaaccttt acaggtgctg
     6241 tggttctcat tgaaatatga tgaatgtgag agtgcacgca gtaacagtga ttcatcttta
     6301 aatttatatc cgagcttttg ctgaagctca tttatatcag gtttcatctt caccaaacct
     6361 ttcgttgaaa taatcaagac ttctctttgc aagagtttcg tatcttaatt ttttatctct
     6421 tattttttca tcgagcggcg gaagaattcc gaaatttgcg cccataggct ggaaatccgt
     6481 cacgctctca ttgcttatat attttgaaag tgctcccatc attgtgaaat ccggaagcgc
     6541 aagcggcgcc ttaccgccaa gcctgtttgc catgtttatt ccggcaagaa tccctgacga
     6601 tgcggattcc atataaccct caacacctgt gatctgacct gcaaaataca ggccgtaacg
     6661 acctctgaac gaaaaatagc tattcagatg tctcggtgaa tcaataaacg tattcctgtg
     6721 catcacaccg tatcgaacaa attccgcatt tttcagcgca ggaatcattg agaataccct
     6781 tttttgttca ctgaatttaa gatttgtttg aaatccgacg aggttataca tcgtaccgcg
     6841 cgaatccgta cggcgaagct gcagaaccgc ccacggacga tgacctgttc tcgggtctct
     6901 caa
//
LOCUS       DAWBSW010000110         6832 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2199_length_6832_cov_2.288918, whole genome shotgun sequence.
ACCESSION   DAWBSW010000110 DAWBSW010000000
VERSION     DAWBSW010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_6832_cov_2.288918"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2295..2304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2661..2670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3566..3575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1969 a         1516 c         1410 g         1907 t
ORIGIN      
        1 acgacggagc cgaagtttat tcccgaaaat gcagttgaaa tcaatcttcc cgacaatgca
       61 catttcagcg tcaggatgat tgactgcgta ggatatatag ttccgagctc actcggatat
      121 attgaaaacg atcagccgag aatggttaaa accccgtggt ttgaaaagga aatccccttt
      181 aatatggcgg cggaaatagg aacgcaaaag gttatcacgg agcactcgac tataggcctt
      241 gtaataacga ctgacggctc cataagcgac attccaagag aagaatatgc cgaagcagag
      301 gaaagagtta tcagtgaact aaaagaactc aacaagccgt ttatagttct tctcaacagc
      361 accgatcccg acagcgaggc tgcgagaaaa atgggatgtg agctttcaaa taagtataac
      421 gtcccggtca tgcctgtcaa ctgccttgag cttgacgagg aaaaaattaa atcggtgctt
      481 gctcagatac tttttgaatt tccgattcag gaggtatgta ttgagctgcc cggctgggtg
      541 acgtcgcttg aaaaaactca ctggctgaag cgtgatattt ttgattatat aaaaaaagaa
      601 gcttccgctc tgagcagaat aagagatgtc aactcattct ccgcaagcct taacagatgc
      661 aaatatctcg gcaaaacaaa catgaaaaat ctcgatcttt ccgacggaag cgctgtcatt
      721 tgtgcgaatg tagaaagcag tatgttctat aaaatccttg cggaaaagac aaagcttgac
      781 attgaaaacg aggaagacct gatgagctgt ataggcgagc ttgccgaaac gaaaaaatca
      841 tatgacagaa ttaaaaatgc tcttgatgag gtcgaggcga cgggctacgg aattgtcatg
      901 ccgactatgg atgagctgca tcttgaagaa cccgagataa tgaagcaggg cggaaaatac
      961 ggagttcggc tcaaggcctc cgctccgtca attcatatga tgaaggtcaa tacaaatacc
     1021 gaggttgcac ctctcgtcgg cagcgaatct cagtctgagg atttggtaat gtatcttctg
     1081 aaagaatttg aagaaaatcc gacgcagata tgggagtcaa acatattcgg aaaatcattg
     1141 catgagcttg tcaacgaagg tcttcacaac aagcttttca ggatgcctac agatgccaga
     1201 atgaagcttc aggaaacact tgaaaaggtt atcaacgagg gttgcagcgg tttaatttgc
     1261 ataattttat aacatatttt ttgttgacaa tcacgaaaac ggtagtataa ttaaacatga
     1321 acaaagttta acaattgttt aaattatcaa aggaagtatt tttatggatt ttgacgtatt
     1381 gaaaattcta tattaccttg ctgtaattct gcttgcaaca aagggcatgg gaattctttc
     1441 gagaatgctc ggccttcctc aggttgtcgg tatggtaatt gccggtctgc ttatcggacc
     1501 cgccatattc agtcagatcg acgccataag ctttaacggc ataatcaatc cgactcagga
     1561 ggaaatggac gttctgaaaa cattttcgca aatcggcgtt attctcattc tcttttcaag
     1621 cggacttgag acagacacca aggagcttag aaaaagcggc tttgcggcaa caatggtagc
     1681 aacgatgggt gttattgttc cgattgcact cggaacgctt gtaacaggct ttttcatggg
     1741 cggaattgga atgcttaaag agcatgaaaa cctcatgaac gctcttcttg tcggatgtat
     1801 cctcgccgca acaagtgtcg gtattacggt cgaaacactt cgcgaactcg gcaagttgaa
     1861 aaccaaggtt ggcacaacga tactcagtgc cgcaattatt gacgatgttc tcggaattct
     1921 tgcattgagc ttgataacaa gctttgacgg cggaggaagt gtcggaatga cgctgctgaa
     1981 atccctcggg ttctttgctg tcggcatcgg tctcggtttc ccgataaaat atctttttgt
     2041 atggatatgc aagcgttatc ctcacacgag aagaacaagc atttttgccc ttgcgatatg
     2101 cttcctctac gcttatgcgg cagaaacatt tttcggtatc gcatcaatta caggcgcatt
     2161 tcttgccgga atcatgctca gcggacttaa agatacagac ttcgttgaca aaaaggttct
     2221 gacaaacggc tatatgatct tctcaccgat tttctttgcc tatatcggca taagcgccga
     2281 tttcagcgca tttannnnnn nnnncccgtg attccgcaac aatcggcttc ggtatgattg
     2341 cacgaggcga ggttgcgctg gctgtttaca caacaggtca tactcttata aaggacggag
     2401 acggaatcaa tccgcttatt gcaacgatta tgcttattgt aatttcgtca atcctctgcc
     2461 cgattttgtt aaagctcgtt ttcggctctc ataatcacga ggattctcat ccaacaaggc
     2521 tccatgacat tcaaactcat tcgcttgaag gcggatattg ataaaattaa cacaacgagc
     2581 tgtgaactct cagtatgaat tcacagctct tttttattat catatatata aaaatgccgc
     2641 accaagaatt tatcggatac nnnnnnnnnn cttggtgcgg cgaaatttta attatttatt
     2701 tgtattcggg ataaaactcg ggaaggaatt ccttatgtgc ttccttgagt tcgttgaagc
     2761 acttaacagc cttgtcataa tcaccaacaa gcggatgaat aagaagtccg ttgattgcgg
     2821 cattctcgtt gcctgttgta gcagcctcaa ctgtgtactt ctcataagcc ttaacaattc
     2881 tgataaggtt gatgatgtgc ttgttgtgga agtcagtaac cttgatcggc tttgcgccgt
     2941 ccttaccgat aacgcacgga gcctcgataa catcgtcatc ctcaaggaaa tcaagagtgc
     3001 cgttattctt aacgttaaca acctgaatat ctcctacatc gtttgcaagc gaattgatga
     3061 gattaacagc ggcaagagaa tacttgtggc cgcctctctt gtcaagaaga gcgggcttta
     3121 cgcaaagctc ctcatcctga tacatcttaa gaagctgctc ctcaatctcc atgcaaacct
     3181 gtgctcttgt cttttcgtcg tcacgctgat gctgaagctt agcctcacgg cagtagtagt
     3241 actgaagata tgatgacggg atagcgttaa cggccttcag gcagtcaaga ccgattccgt
     3301 cgtcctgaat attagccata accttagcct tatagccctg gttgatcatg tctgtgataa
     3361 gctccttgcc gtccttctta atagaagtta cccagctaag gtggttaagt ccgacataag
     3421 taatatcaac gtcgggacct gtagtctcct tgatatcgtc aagcatatca ataggaacat
     3481 tgcaaagacc gatattctta acgttggtgt tgttaagaac aaactcggtg ataaggcccg
     3541 acgggttagt aaagttgatg agccannnnn nnnnngataa ggttatactt cttggggatg
     3601 ctctcatcaa gataacgtgc atgaagcatc ccgacacgaa tttgagtaac aacgaagtct
     3661 gcaccctgaa gcgcaagatc aagatcgtct gtcataacaa cctcacagtc aacgcctgct
     3721 tttttaagca tacgaacgca aagattgccg acgattgtac gctttctctc gttgatatcc
     3781 ataaaggata ttttcttaag cttaagagaa tcggatgcct tgagaaatcc gtcaacaagc
     3841 tccggacaat acgtgctgcc cgaaccgatt acagctacag tgatttcttt catatgtaat
     3901 tacctctctt gttatatata ttgctggaaa atccagttga tacagccggt taccgggggt
     3961 tgagtcagct taatgaattt aagctttctt ccgccggccg ccgacgcctt ttccataagt
     4021 gctttaatat aaatttccga cgggagctta gtatgaattg agcccgaaag aacgacctca
     4081 acaacatcgt cggtaaaatt attagtgtta agatgtgcgg caatgagagc ggcacctctt
     4141 gaagccattt tgtcaattac atcaagaaca gctttatcgc ccagctttgc ggcatcaaag
     4201 aagaaatcaa taagacgcat gataaacttg tccgcatcgg aatcctcaaa ccttgaaaca
     4261 agcgaaagaa attcttcctt tgaggaaagc gagtactcat caaacaccat cttggtaacg
     4321 gcagtctttc tcaatccgag ataaacgtcg tcataaacca ttctgtaggt ttcgctcgca
     4381 atccaatgac cgttgccgat atcgcccgaa aattcgccga gacccgcaag ctgaagcatc
     4441 ttgccgtcgt tatcaatcga gttacagcat gttcccgtac cgcagttata tccaatagcc
     4501 gcagcacctg ttgagccggc tttaacaaca atgaagccgt cgttatatat ctcaaacttt
     4561 tcaaggccgt aatcaagaag acgctttttc atttcgtcat gctggaaagg atggtcgatg
     4621 ccggcaagac ccatgagagt aaaatcaacg tcatgaattg aaatgtttgc ctcgtgaagc
     4681 agatttttta tacctgccca gatgatatcg gccgcttcat caaaaccgtt ttccatattc
     4741 tcatggttgc tgcccggtcc cttgaccata gcaacgatgt ttttcttttc atcaaaaagc
     4801 gcataagcgg tcttggttcc gccgccgtca ataccaatgt agtaactcat gtgtaactcc
     4861 tttgtttgaa aatttttgca atcggcaatg acaccatcat aattgcacat ataaccttta
     4921 atattatact atctttgtct tttaaaaaca atagcttttt gtaaaataat ggttaaaaag
     4981 tacaaaaaat caacactgta aagaaaatca gacctttttc ataaggtcaa ttccgaactc
     5041 aatttctgtt tcgaggctgc cgatattgta aagcttctcc tgatcagact tacccgtttt
     5101 ataataatag ggcgtcattc tgaaaagatt cataatatct tcattgccgt aaacagtaat
     5161 tttttcttta atttcaacgt gttcttttat ttcaaaaccg tccggagcac ttctgtcaac
     5221 ctcattaaga taaggttttt cataaattaa gtctttgaga cccattaaat gtctctcaag
     5281 aggataagct ctgagccggt atccgttgct tttgagaact ctgtgtatct catcggaatt
     5341 gtgcggtgca aaaaccgtca tcgcaaaatc gcaatattca tcttcaatcg gtaaattgaa
     5401 cacactcgca acggcaagcg agatattttt actgcgcttg cttcccgcaa tcaacgcctg
     5461 ctttgaaata tcaatcccgc cgtaggctaa atcagggaaa gcctttgcga ctctcgaggt
     5521 gtagaaacat tcgccgcagc cgagatcgat caagcgcata tttgaagaag cggtctcccc
     5581 tatcatgctt gttatcttat ctgcaagaga atcgtaatat cccttgtcaa gaaaatccga
     5641 gcgggactta accattagct tatcgtcgcc atgccttttt gacgaagatt tttgagactg
     5701 caaaagattg acatagccct gttttgcaag gtcgaaacaa tgtccgcttg cacagcgcag
     5761 cgagccgccg tctttttcaa ggcggcagcc gcatacggga catgaaaaat taaaactcat
     5821 attatgcctt cttcaattca tcctcgggag cctttgcgca aggacattca atcggctctt
     5881 tacggatatt gagcggagca acatatctta ctgcatttgt aataacagtt tgtacgtttg
     5941 gatttttaaa tgtaggattt gtctcatgac ccggctggaa atagaatacc tttccgagtc
     6001 ctctgttcca tacgcagccg gaacggaaaa cctctccgcc gccgaaccag ccgataagaa
     6061 caagctcatc cggatcggga acgtcaaatc tttcaccgta catttcctcg tgctcaagat
     6121 agaaatattc acctaagccc tgagtaatat gatggttagg agcgatattc caaacacgtt
     6181 ctctgtcacc ctctctccac ttaagagaac aggttgtacc gagcgttttg cgtaaaatct
     6241 ttgaaaaatg acctgaatga agaacgataa gacccatacc tctgagaatt ctgtcgtgaa
     6301 tcttttcaac aatctcatcg ggaacctttt catgtgcaac atgaccccac cagataagaa
     6361 cgtcggtatt atcgagcacc tcgtctttga gtccgcactc aggcatatcg agagttgcac
     6421 aggttacgtt tatatcatcg tttttgccga gaaaaccggc aatacaagcg tttattccgt
     6481 caggataaac tgcaagaaca ttgtcctcct tatcctgaac atgctcattc catacggtta
     6541 cgttaatcat tttaacgacc tcctttttct taaaatgata tcacattggc atgataattt
     6601 caactgtttt gcaatgatat ttatttcccg tcaatataat ttccgtctcc cgaaatatac
     6661 cgtaaataat cgatcgaaac gcaatcaatt gccgtgccgt gaatgatttg atgtctgagc
     6721 atcgaacccg caaaaccgaa accgaaaggt ttaattgttt tatcgtggaa caactgataa
     6781 aagctccaat gttcactttc cgtcggcttc tgcccgtttt gcatcagaaa cc
//
LOCUS       DAWBSW010000111         6740 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2235_length_6740_cov_2.634555, whole genome shotgun sequence.
ACCESSION   DAWBSW010000111 DAWBSW010000000
VERSION     DAWBSW010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_6740_cov_2.634555"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2135..2144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1715 a         1671 c         1448 g         1896 t
ORIGIN      
        1 ccgtcctctg tgaacatctc ttttgtttct tcgggcattt tgtagtatcc gagcataacc
       61 gtcggtgtgt ttacctgaat ttcgccgttc tgaatacgaa ccgtgcaaac gtccgtaaca
      121 cgtccgacag actccctcga agccgtgtta tcgggaacgg aaattctgca tcctgcctcg
      181 gtcatgccgt aaccctgacg caggaaaatt ccgagcttct cgtattcgtc aataagctcc
      241 gggttaagat aagcgccgcc ggaaataagc cacttgatgt ttctgccgaa cacctgagcg
      301 accgcatcct taacgcttgt ctcgggttct tttgcaagaa taaccttgac tcgctgcaaa
      361 agagactgag cgatcatcgg aactacacga acgaccttcg gctcaaagat tttgagatta
      421 tggatcaaat ccatcatcga atcgttcaag caaacctgca agccgtcctt aagatttttt
      481 atatagtctc ccgagaagca ataaatgtga tacatcggga gaaccgaaag cgaaacatcg
      541 ttcggcagga aaatatctgt gcaatagtcg tgatacatga tatttccgac aagggcaaga
      601 gtcgaaagct caacgccctt ctttatgccc gttgtgccgg atgtaaaaat gataacgcag
      661 caggcatttt tatcaaccgt aatgtcagaa agcgccgcat actccgagtt ctcggaataa
      721 tcggtataaa ttctgttaaa ttcttcaccg ttgccgagat tgacggagaa cctgaggaac
      781 gggcaaagct ccttgagctt ttctgcgttc tcggtaaact cgtcttcata caaaagcatc
      841 tcgatatcgg cacgcttgaa aagctctgcc gcctcctcaa cggagatatc cggagcgaac
      901 ggaacggcaa cattaccgct gataagaatg cccgaaagac aggttatata ctcgtaattt
      961 gattttccga taatcgcaat gtgcattctc ttatcggaaa gattacgaat ccagcggcat
     1021 accgcaaggc tgtcgcttct cactttttta aagcttcttt cttcgatttt tccatctctg
     1081 atgaacttga tgaacgttgc gtcgccgaat ttctcggcac cgttgtcaag cagctctctt
     1141 acggatggtg cgtcaaattt tttcatgtcg tttctcctta ttaccttttt taaataataa
     1201 ataagagcaa ttcagtgcag gtcatcattg aaatcttttg caatgtgata gcactcaaat
     1261 aggattatat cacaataaca gtataatttc aacatatata aacaaaaagc tgcggcctcc
     1321 gaagaagccg ctgtaagaat tatttttcgt tttctttaat ccattttacc atgtcgtcaa
     1381 ccgactgttg aacgctcatc ggttcatatc ccaattcttt tcttgccttc tcaatcgaga
     1441 aattgcaatt agacataagc tttctgatcg aataacgggt gaaaagcggc gttgtcttgg
     1501 tgaaggaata atatttctcc ataagcggcg caaacggtct taccatgcca taggacattt
     1561 ttaatgacgg cgctttcttg ccgcaggcct tagcaactgc cttgataaag ccgtcggtcg
     1621 agatcatctc gccgcaaaga atatagcagt cgccggcctt acctttctcg gcagcagcgt
     1681 gcattccctt cgcaacgtca cgaacgtcaa caaagttgta tgcgccgaag ctgagcgata
     1741 ccggaaaact gcctttgata aacatacgta ccatttcgcc gacatttgaa accttgaagt
     1801 cataaggacc gatacatgca cccggatgaa caacgacagc gtcgatattg ccctcgtgaa
     1861 cgttatcgag aacgaactga gttgcaatag ccttagtctt tgcgtatgtt ccgtcgagat
     1921 ttttcgggct gaaatgatca agctccgtca ttacctgatt gtcgggaagc ggcggaaaag
     1981 catcaacact cgaagcgtaa acaagtctgc ggacattgca tctctgacag gctcttacta
     2041 cgttctttgt tccgttgacg ttaactctcc atgtgagatc ctccataccg gggttgatat
     2101 caataactcc ggcaagatgg taaacaacgt caacnnnnnn nnnncaagcg attcggaatc
     2161 ggtaacgtca ccgtagacct tttcgcagtc aatgccgtca aatacgggaa catctttacg
     2221 gataagaata cgcacctttt cgccgctgtc aacaagctcc ttcaaaagcg catagccgat
     2281 atgacccgtt gcacccgtga taagacttat tttttccatg acttattctc ccttcatttc
     2341 aagaagcttc tgctttccag taaaggcatc aacggaaagc ttaacggaat caaagatagc
     2401 tgattctata tcgtcctcct gaacgccgag agaaacgctc cattttttat tgataaatgt
     2461 gttcatgcac atgatatcag caagataaac cgcattgatg cccgactcga cattgcgctt
     2521 tttgtactcg ttcatcatgt aaacgccgaa tgccttgaac atccactttg ctatatgaat
     2581 gatctttctg ttcggctgtc ccatagccct gtgaacgatg cgaaggaact cgtcccatgt
     2641 gaggttataa tagcctatcg gatatgcatt gccgccgaca ttcttctcgg ctgctcctac
     2701 gatagcttcg ccaacctgac gaacggtaac catagctgtg ccgcccttag gatacattgt
     2761 tgtcttgccc atgtttgtaa gctgctcaat aagaattacc caaacaggct ttctgcccgg
     2821 ctgagtgccg aagatatacg gaagctcaag cacggcaacg tccatctcgc cgtcctttgc
     2881 aaatgaaaga gcaacctcct cctgctcgat acggcttctt atatagtgat gcttctttgt
     2941 aagctccatc tccgggaatt cttttgcgaa ataagcaaag taagagccga ggataacact
     3001 cttttttata ccgacctgct ttgcaattgt aagaagatgt cttacaggct cgatattgta
     3061 ctttctgtat gcctcataaa caggtgccgg gaattcaact ctctcgtcaa cgcccgctgc
     3121 aaatacgaag cagtcgcagc ctgtcatcat tccaacaagc tcatcatcgg aaagctcaag
     3181 atagttgccg aaaacaagct ccatctcctc ggggatcggt gctccctcgg gaagcggagg
     3241 aagggcaact gattttacgg aatggcctct tttaatgaac tctgcagcgg ctgccgagcc
     3301 gagaagacca gtgccgccaa tcataaacac tttcataata ttcacctata gggcaccgtt
     3361 tccgatgccc cgttatcctt tctgaattta ccagttgatt gtgaaatcgg actcaatgcc
     3421 gaacggcgca ttgacattgt tgcctgcaac agttacgtca agctcccaca catagtaata
     3481 gttgatatgc gtaacatttc ccgtcttagg gtcaatcgtt gctacgattt taccgttgcg
     3541 atatgctatt ttaacgttgc cgtcaagcgt tgacttgagc gggcctgcgt tgtcaagaat
     3601 cgttgaaacc tgaacgatat tgaacgcctt accgtggttt cctgctccgt gagtcggatt
     3661 ggttgaggct gcgtccggct taacatggat cgttataaca tacttgccgt tcttctcggc
     3721 gagagttgca cttgaaatgt tagctgttgt aagctttgac gaccagtttt cgttttcaac
     3781 agggaacaaa gctttcatat cagccttgga gagcttgcgg tttgtgtcgc ccttcttctc
     3841 gcccatgttc ttcttaacga gtgaattgat aactcccgaa aagctcttaa gcataccctt
     3901 aagagtaaat gtacccgcct gagaattctt ctcgtagttc tttacgccgc ccttggagct
     3961 tgtcttaacc ttattaaccg atgtgttgaa atagctaaga atttcagcct gagttgaggg
     4021 ctttgaagat gcaggcttgt ttgccgaagc gctgccgcct gtatttccgc ctgcgctcgg
     4081 tgttgccgaa ccgccgttat ttgcgtctgc ggccgtgctg tcgggctcgc ctgttgccgg
     4141 gtccgtcgga gtctccggtg taacagaaga atcgcccggt ataacgtcac ccgaaccgct
     4201 gcttccgttt gaaccgccgc tgccgtttga agctgatgat gccggagcat attttgccgc
     4261 ctcaaccttt gcgtcggcaa gtttatttgt tccgagaaca gatgaaacgc agactgcaac
     4321 aacgcagaga aaggccgtaa tgcatgtaac tactgttttt ttcatatata tcactcctgt
     4381 atttttaaat acttatttct cgccgaaatc cttgaagata tccatgccgt aaacgttgtg
     4441 ctcgtccttg gtgtggttcg gataccaaac ctgagcgaag aacggattct tctttgcaat
     4501 ttcgtcgtaa ttccacggaa tcggatcgca ctccgggcac cagtgaccgc cgttgagaac
     4561 aaggttcggc gtcatctcaa atgtatgacc gaatgcacac tgccacttga gcggtgtgaa
     4621 aaggtcgccc ttcgtcatgc tctcactcag gcacttgccg cccctgaact ccgcagcctt
     4681 cttcatatcg tcaaggtcaa gctcggactg cggctttgtc tcgtcatagc cgtggtcaag
     4741 atatgtcgga gtcatggagg ccttctttat ctcaaactta tcccaggtct tggggatgtt
     4801 cttccaatcc tcatatgagc cgtaatatgc gctgatacgc tcggggactc tgttcttgat
     4861 ccagttcatt gtaccgaata cttccttgtt tgcaaggccc tccataagga acttcttaac
     4921 aagtccgccg ggagcaagct tagaaagctt gtagaacttc ggcaaggtct ttgacatatc
     4981 gtcaaaatac ttcttgatgg gaacattgtg tctgaagtgg aggtaattct caagcttgtc
     5041 ggagtcggca taatactgac cgtggaagtt tttgagaatg aaccagttcg gtgcaaagag
     5101 cttcttcggt gaacccatgc cgattgcgcc gagaagatac tcctcaaact caaagtttgt
     5161 gatgcggtac tgctcgcccg aaccgatgtt atagaacttt ctccagaaat cctcgggaac
     5221 ggaatcctcg cagacatttg caagaagaag tcccgaatcc tcgatagttg cccactcaag
     5281 aacgccgttg attggaacgt ggaacattat cgggtcaatg ttcttgagga tacccggata
     5341 aaggataccc gactgacgaa gcgataccca atacttaagt cccgactctg cgataatatg
     5401 ctcggctcta accttgctta cggcatagtg atcgtaaatg ctgattttaa ttgggtcacc
     5461 cgttctgccc caatggatgg gcggattacg gtcgccggtc tctgcaaccg ttcctatgta
     5521 aacgaccttg attgcgtccg gatcgggctg agccttaacg gcgtcaacga tgtactgcat
     5581 agcagtcgta ttggtcttga ttgttctctt cgggaaatag tcggcagccg gagaaaccat
     5641 tccgccgacg tgaagaacga tgtccgagcc tgttacacac ttgcgaacat catcgtaatt
     5701 tgtaaggtcg ccccaaacaa ttttgacggc aggatcgttc tcgtagtcct tgaacttgat
     5761 gcgattcttt tcgcttcctc tgaggagaag aacaaggttg tacttatcct ttctcttata
     5821 aatctcctta aaaccggcat atcccatatt acctgatgca ccggtcaaaa atacggtttt
     5881 tttgctcatc ataatcctcc caatttataa ataaataact ctttaagcaa ccgtcgatta
     5941 aagcacagca cgcagcgctt caatccaagc ttcggtcact caatgtctat tttattatag
     6001 gtcatttcac gcttcgttgt caagcattta aacgtcaatt tcgacaaaat attaacatgc
     6061 cgtgaaatta aacaggatat aattactgtt tgaaattctt tacacgtcct ataacaaaag
     6121 ccgcctgcaa aaagcaagcg accttttatg ttttattaag caaatgtgtg cgataattgc
     6181 attaaactat ttttgcgata atctgacttt cgtattcgct gtcgtgaagc tcgccgagat
     6241 ccgaactgta cgcttcatgc accattttgt tcctgacggg aacctttttc ttcgctttca
     6301 tgcgatacca ctcatattca acatcaacat gaccgatatc taccgcctga tagccgagct
     6361 tatgcaaatc ataggcaagg caggttgccg cagggccgag agccaaaaga atgagtacgt
     6421 ctttatcgag tttttttgcc tcatcaagaa tttcgtcata ctttgaaaat gcctgccttg
     6481 acgggccgag aatacgcttt atagattttg cgttgtcgag cagatcgtta ccgacgccga
     6541 gacggctcat ttcgccctcg attatcacaa cgtctctgcc gtcccatatc ttcttgatat
     6601 gctcaaagat ctcgctgctt tcctccttgt gccgcaagcc gatatactga cgtgtcatgg
     6661 aagcgttata atatttcttg cccttgataa ggtacttata ccaaacgcct ctgaatcggg
     6721 caagatgatt tttccaatga
//
LOCUS       DAWBSW010000112         6687 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2257_length_6687_cov_2.685464, whole genome shotgun sequence.
ACCESSION   DAWBSW010000112 DAWBSW010000000
VERSION     DAWBSW010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_6687_cov_2.685464"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    874..883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1932 a         1310 c         1606 g         1829 t
ORIGIN      
        1 agggcacatg gctatgatgt ttatgaggaa agaaacgcgc tcaattatct tcctttgaga
       61 aggtttctcc ttgaaaactg cgatgccgta tatccgtgct ccgatgttgg aacggaacac
      121 atcgtttcaa gatatcccga gtacgccgat aaggtgaaaa ccgctcatct cggtacgatt
      181 gatgcaggac tgggaacgca gtccgaggag ggcttacaca ttgtttcctg ctcgcttatt
      241 acggatatta agcgaattga taagataata gatattctcg aaaagcttga aaaaacggga
      301 aaatataaaa taaaatggac gcatatcggc gacggaaaca gacggtccga gcttgaaaaa
      361 agcgtcagaa aaaagctgaa gaaaaccgac acggaatttc tcggaaacat tccgaataac
      421 gaggtttatg aattctacag gaataacccc gtcgatcttt ttataagcac aagcaaaagc
      481 gaaggcctgc ccgtatccat gatggaggcg gcatcgttcg gaattccgat cgtttcaacc
      541 gatgtaggcg gagtccgtga aattgtggaa agcggatata acggtcagct tctcggtgaa
      601 agagcatctg ccgacgagtt tgccgatttt ataaaacgat attacggtat ggataaatcc
      661 gaaagggatt tctacagaga aaattcaaga aagctttggg aaaggtcatt caatgcaggc
      721 aaggcatatc ctgatttttt ctcaatgccc gtattgtcac gatcatgagg aaaggataat
      781 tatgatatct ctgaaaaatg gtgttttgag aataaactcc ggcgatcaga gcagggcaag
      841 acgcttgtat gagtttgcat ttttgctcaa catnnnnnnn nnnacatggt aattgtcttt
      901 gctttttaca gggttccgat aatcgctccg gctgccgcgg tttttatgtt tgtggcgtcc
      961 ttcctcgtgc ttttggaaaa aagtaagaac aggataatga taccgtatat tacggtatgg
     1021 tatttgacgc tgatattata cggaggtctg tccggattat gggcgcagtt ctttacctca
     1081 tataatataa gctttgtgat aaagctatgt attattctgc ttatctcaac ctctgttgcg
     1141 atttatgttg acacaatgga ggatctggac agattgatgt cgctgtttat tgcaggtgca
     1201 gttataataa cgattctcga atttcttgcc gttccggcag acggttggaa aaacggaagc
     1261 gtcggaagct atttcagcgg caacaatccc aatgatataa ccgtctggat cgatttcgct
     1321 tcggcggttg cgttctacag agcctatgtc aacggtaaga aatggatgta tccgttggtg
     1381 gctgttttcg ttgtcttttg cgctttttca agctcgcgaa agggtctgct tgcctcgctt
     1441 gcggctccga taatgatagt ttttctctcg gtcggaaaga aaaattactt tttgagaata
     1501 gtagcggcgg tgacaatcgg tgtagccgtg ctgcttattg taatggaaaa cgatacactc
     1561 tacacggtta tcggtcgaag atttgacagt atgattgact atataaaggg cgaaggaaca
     1621 gatggaagta ttgctctaag acggagatat attgcagcgg caaaaagtat gttcaacgaa
     1681 tccccgatta taggaaaagg aatgggcaac tattcccgta ttcttgaaag tgaatacttc
     1741 ctcggcaact tttacagcca taataattat tggcagattt taagtgagct tgggctgata
     1801 ggctttttga tttatttttc aatgtatgta tattgtgttg tcattttttt gagagcatat
     1861 tttgtggaga aaagaaaaat ttcggctttg tttataacgg ctattacgat gatgattgtt
     1921 cttgacagcg gaataatcag ttatagctca aaatacggac agcttgttat cgcaattatt
     1981 tattgcgcta cctatgctat acattccgac ggcggcagaa aatacagcat gaaacgacag
     2041 taaatatttg gagtggttaa agtgaaatca tattcggaca gaaaaaactc aaagggaatt
     2101 gaaataaata ttctcgcttt tttcaaggag ctttccaaaa agctttggat cgttgcgata
     2161 attgcagtta tcggagcgac aataggcggt gttatcggcg aggcgacaaa gagcgagact
     2221 tatacttcaa cggtatcttt tgttgttaac tccgcaacgg aaaacggaca ggtttcaagc
     2281 ggtgaaatca atgctcatat caatatggcg ggcacattca attatattct ttcaagcctt
     2341 gcgttgaaag aggcaatcgt tgaaaaatgt cctgaaaagg ttaacttcgg cactgttgac
     2401 aaaagtatct cggtcaatgt tgtttcctcg acaaatgtta ttgttatgtc ggtgacgacg
     2461 ggtgacccga agatttccta cgcaattgca agcacggttg ttgatgttta cggcgatata
     2521 gttaccgaaa gatatcctaa tgccaagctc actctttgcg acactcccgt gcttccgacc
     2581 tatccgaact caaacagatc tgttgtttca cttgctttga tggtcggctt tattgccgca
     2641 gttgcgagct gtgtggtttt cttcattatg ttccttgtta aggatacggc aaagaccgca
     2701 gacgagctca gcgaaaagct ggatgtgcat attctcggct cggttcagca gattacgaat
     2761 aagaataaaa ccgcaaaggg cttgcttgtt acggacagaa agaacggctt ctcttttatc
     2821 gaaacatata aggcaatcag aacaaaaatc gaaaataatt ctacaagaac gggcaataag
     2881 gtctttcttg taacaagtgc ttgcgagaat gagggaaaaa cgacctcctg cgttaatatt
     2941 gctctttcaa ttgcgcagaa cggcaagaac gttttgataa tcgatgccga cctcagaaag
     3001 ccgtcggtca gcaagatgct ctcacttcct actacggaat acggactttc cgatgtgatt
     3061 ctcggtaatg tggagctgaa ccaagccatc aagcgtgtta caagcttcaa tgtctgtgtg
     3121 cttgccgatc agaagggcgt taataatccg tcggagcttc tttcaacaga aaaaatggag
     3181 tctataatcg aagaagcgcg caaggaattt gattatgttc ttattgatac cgcacctgcg
     3241 agcgttgtta cggatacctc ggttattgca ggcttcgtcg atgcggcgat aatcgttgtc
     3301 cgtgaggact tctcgcctta ttcgagaatc agaatgtcaa ttgaggatat cgactcgaac
     3361 ggtgcggaaa ttatcggatg tatcttcaac gccgatacaa gcaacctcag caaaacgagc
     3421 cgctacggcc gtcgctacgg cggctatggc aagagatacg gctacggtca gtcatcgtca
     3481 aaaaaataat tattgaatgg catacaaaac ctctttcaca tattataaag cgaaggaggt
     3541 tttttaatgc gcgagcttga aaaaatgatt gaaaaatatc ggcaggagct tgttgaattt
     3601 tcaaaacaaa aaccgattgc cgatgaggaa attttcgaag ccgaggaaga gaatactgtt
     3661 ccggcaatgg caaatgcttt gccttttgta agcaatgcgg gtgcaaacaa ggaaacggat
     3721 ttttacaaga attttgagga tttccaaaac agaaacccgt ctaagggcag tatgcgcgtt
     3781 caggtctcat cgggaggaag aagctacccg ataatcaatg caattgttaa gataagcgtt
     3841 ccgctcgaaa caggggaccg ggaaatttat tcgggatata cggatataaa cgggatcgtg
     3901 gacaatattg ttctgcctgc tcccgacacg agctattccc ttgatgagaa gaacacaacc
     3961 gttgagcctt atgcaactta tgaggtcacg gtcactcacc cggaatttgc aaagtcaaaa
     4021 ttttttaatg ttcctgtttt tgcgaatatc aaggcaattc aaccggcaag gcttgttccg
     4081 ctgacggaaa cgggcgggga accgggcccg gtcaatattg cagaacagcc tatgagactt
     4141 tacggaggtg acttatgatg cctgacagac ctatcgttcc cgaatttatt actgttcatc
     4201 tcggcaaacc gaatgcggcg gcaagaaacg tccgtgtgcc gtttgcacag tatataaaaa
     4261 acgttgcatc aagtgagatt tatccgacat ggccggaaaa tgctctccga gcgaatattt
     4321 atgccgagat aacttttgca cttaacagag tttatacaga gcattacagg tcaagaggct
     4381 atgatttcga cataacaaat tcgactcaat tcgaccaata ttatgttgag ggcagagata
     4441 tttttgaaaa cataagccgt gtggttgatg atattttcaa tgattacgtt gtcaaacaag
     4501 gtcagataca gccgtatttc acccaatact gtgcaggaac ctgcgagggg ctttcgcaat
     4561 ggggcacggt tgcacttgca aatcagggct acacaccgta cagaatactt cagtattatt
     4621 acggaaacga tattgatatt gtgaaaaatg cccctataaa atcaaatacg ccgacctacc
     4681 ccggaacgcc gttaaggctt ggaaccggcg gcgaagaggt tattgaaatt cagcgtgagc
     4741 tgaaccgaat tgccgcaaac tatccgtcaa ttccgaaaat ttcgggctca agaggatatt
     4801 ttgagcagac gactcaaaat gcagtcagac agttccagca aattttcaat cttgagtccg
     4861 atggggttgt gggcaaggat acatggtaca aaatcaaacg aatctataac ggcataaagg
     4921 gtctttcgga gctttattcc gaagggatca caataagcga agccgaaaga aaatacgaga
     4981 gagttctgaa gaaaggcagc agaggtccgt ccgtaaaaat cctgcagtat tatctttgtt
     5041 ttctcagcta cttcaatctg aaattgccgt atgttgcggt tgacgggatc ttcggtgatg
     5101 aaacgcgtga cgccgtgctt gcatttcagt cgctctacgg acttgacgtt gacggaatag
     5161 tcggcagaga cacatgggat atgattcaga acgcatatgc aggtgttctc acttcactgc
     5221 ccgatgaata caggagctat tcgtcattgc tttacccggg atatatcatc acaacgggtg
     5281 cgagcggtaa ggtagtcgaa cagctacaga cttatctcaa aacgattgcc gccaacaatc
     5341 cgtcaatacc ggatgttacg gtcgacggct attacggcga agagacgaaa aaagcagttc
     5401 ttgcggttca gaagcttgag ggtattgagc aaaacggtca ggtcggtgtt ctgacatgga
     5461 atgcaatagt aaatctttac aatcagttta gataataggt atattttaac tcagtccgaa
     5521 aataataaca ttatcaattt tacggagaaa tgctgatgga taatgtgttg ttttcggata
     5581 tttttgatta taacgaaaag cttttggaca agtatttcag aatcggtctg aactacgatg
     5641 tcgtagccac aaaattcaat gcgggcggca ggaaagcgaa gctctatttt gttgacggat
     5701 ttatcaacgg tgaaacttcc gagcgtgtga tgaactttat gatgcaggca agcggggttg
     5761 ataaaggtgt cgccaacgcc gaagagtttg cgcaaaggct cattgcctat atggaaattg
     5821 aactgagcaa ggatattgaa aaaattgcca aagttgtata ttcgggagcg atgctcgtta
     5881 ttgttgacgg ttttgacaca gggttccttg tcaagacgag gagctatcct aagcgcagtg
     5941 tatgcgaacc cgataacgat aaggtgctca gcggagcgca tgacggtttt gtagaatcta
     6001 tccttataaa cacggcgctt atccgtcgcc gaattcgtga ccgtgatttg gtaatggagg
     6061 tcagagaggc cggtgcgaaa tcaaagacag acattgcaat atgctatctc aagggaaagg
     6121 cggacgaaaa gaccgttgac gatctcagac agagaattga taaaattgag gtcaatacgc
     6181 tcaatatgag tcaggagagc gtgattgaat gtcttgtcag aaaacagaaa tggaatccgt
     6241 ttccaaaggt cagatatacg gaacgccccg atgctgcggc ggcgagcatt gccgagggaa
     6301 gcgtaattct ttttgttgac aattcgccga cggcaatgat aatcccgacc ggcttttttg
     6361 attttttgca ggacacaaac gattattatt ttccgccatg cgtgggcaca tatctgcgct
     6421 ttgtcaaggg attgattttc gctcttgcat tgcttcttgc gcctgtatgg tttttgctga
     6481 taaaaaatcc cgatcacatt ccggcatggc tgagcttcat tcaaatcaag gagtattatt
     6541 cgctgccaat cattgctcag cttttgataa ttgaggtagt cattgacgct ttgaagcttg
     6601 cttcgctgaa taccccaagc tctttgagta attcattcag cgtcatcggt gctcttgttc
     6661 tcggtgatct cgccgtcagt gccgaat
//
LOCUS       DAWBSW010000113         6642 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2274_length_6642_cov_2.800820, whole genome shotgun sequence.
ACCESSION   DAWBSW010000113 DAWBSW010000000
VERSION     DAWBSW010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2274_length_6642_cov_2.800820"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3533..3542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5983..5992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6263..6272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2186 a         1343 c         1604 g         1479 t
ORIGIN      
        1 ctcacaggac ttgatacagg gcatgacgct cgccggcgtt ctttttgatg aggttgcatt
       61 gatgcccgaa tcgttcgttt cacaggctga ggcacgatgc agcgttgagg gctcaaagct
      121 atggtacaac tgtaatccga aaggaccgac gcactatttc aagacaaaat atgttgatgc
      181 gataaaggac aaaaatcttc tttatctcca tttcacaatg gacgataatc ttacgctgtc
      241 cgaagccgtt aaagaaagat atcgccgaat gtttacgggc gttttctatg accgaaatat
      301 tttaggctta tgggtaacgg cggaaggcaa gatatatttt tcgtttacga aggacaatgt
      361 aattgcttct gccgattggt atgcaaaaga cggaaacggt aattatattc acccgctgag
      421 aaagaatata tcaatatgca ccataggcgt cgatttcggc ggaaataaat caagcacagc
      481 gtttacatgc acggcattta caaagggctt tgccgagtgc gtgacggtca aggagcggcg
      541 cattaaaaat gagatcgacc cgacacagct tgaaaaggag tttgttgact tctgccgaga
      601 gtgcagagcg gaatatacgg tgcttgccgc ttattgcgac tcagccgagc aggtgcttat
      661 acgagggctg agaaaggcgg tcatgaacga gcacatacct attgcaataa ataacgcccg
      721 taagggtgaa ataatagaca gaatacggtt tggattgacg atgtttgcac aacatcgttt
      781 ttttattgtc tcaaattgcc cggagacgat acaggcgtat accgacgctg tatgggacga
      841 taagaaagag gacgtaaggc ttgacgacgg gacaacaaac atagataacc ttgacagctc
      901 ggaatacagc caggagccct acatgaaaac aatgatagac ttaacgaaga aaagaggata
      961 gcatgaatcc gcagattgta aattatttaa agagcaaagg aaatacggtc attgacgaaa
     1021 acattgatga caagcttgga acgtgggagc agtggtacag aggacacgtc gaccgcttcc
     1081 attcatacaa gatatacgag ggtcagaaga acctcaatca gaaaaggcgc acgctcaata
     1141 tggcggctcg agtatgccag gattgggcgg acctgctgct taacgaaaag gttgagcttt
     1201 cggcatcgga taaatacacc gaggaagttt tgcacaggct tcttgaacag gttaatttct
     1261 atgtcagagg gaacaacctt attgaagcgg ctttcgcaaa gggcggcggc tttcttatcc
     1321 aatattggga cggaatgaag gtcaatcaaa agtacattac gcaggataaa ttctatccga
     1381 tttcctttga ttccggcaag gtaacggaag cggcttttgc ttcggaaata acaatcaagg
     1441 gtcagaccta tacatatctt gagacgcata caaaagacga aaacggcttt tatgttgttg
     1501 aaaataatat tttaaagtcg gaaagtcaaa gcatttccga aatgcccgag atgtacgggg
     1561 aattgggctt ggatcagtat atttacacgc tttccaaaac gccgacattt cagcagatac
     1621 gcccgaacat tgcaaacaag gataaattcg attcgcctta cggaacaagc atatttgccg
     1681 gtgcaacgga tgtccttgag ggcgttgaca ttgtatatga cgcttatgtt aaggaaatcc
     1741 ttctcggacg aaagagaata tttgcgaggg attcagttac caacgttcac attatggaga
     1801 acggacaaac ggtcaaggtg ttcgacccgt cggacgaggt cttttatctg ctgcccgaga
     1861 acaaggacga cccgtctgca ccgccgatta ttgagagcaa tatgcagttg agagttaccg
     1921 agcttgatgc ggcgatgcaa acgcagttga atttgctgtc acaggcgtgc ggcttcggag
     1981 caaacggtta caaatgggac agcggcaatg tgtcaacggc tacacaggtc atatcggaaa
     2041 acagcaaaat gttccgcact ctgcataagc acgaatcggt tctgagaacc gcaataattg
     2101 acatggcaca cagccttttg aactttgaag cgatatataa cggggataaa agcattgatt
     2161 taaacgcaac gataacggtt gattttgacg attctatcat cgaagatacg gcggagataa
     2221 aaagacaggc tatgcttgaa ttcacagccg ataatcaata tttacagtca aattgagctt
     2281 gatctcatca aggaaatagc gagaagattt gatttgaacg atgaaatcgg cggtacaatg
     2341 gaatggaagc tgaaaaagct tgatgaattg ggtgttctca atgccgacac ggtaaaggtt
     2401 attgcctcat attcggaaaa gagcgagcag gaaatactca atatgctcaa aaaggcgcag
     2461 cttgcaaaca tcaatatgac cgagcttgaa gaagcataca ggcagggcag tatcacggtt
     2521 gacccgatga atataaaaac caattcggca tttgccgagg tcctggagct gagctataag
     2581 gagctgagca atacatacag acttattcag acaaaggcgc ttgaaagcgc aaaacaggcg
     2641 tatatgaacg ttatcaaccg ctcatatatc gaagtcgcga cgggaaccta tgactatcag
     2701 acggcgatca agcgagccat aaaggatatg gcaaagaacg gcataagcgg cgcaacatac
     2761 aagagaaacg gaaagcttgt tcaatactca cttgagggaa cagtaagacg tgatactctg
     2821 acggcggtca ataagcttgc caacaagtcc tcggagacgt tttgcaaaga gctcggtgcg
     2881 gaatatgttg aaatttcttc gcacttggga gcgagaacac accctacaaa tcccattgca
     2941 aatcacgcag gctggcaggg caaggtgttc aaaatagacg gaagcgacaa gaaatacccg
     3001 aaccttaaag agagtacagg ctatcccgat gacatcttag gcttgggcgg tgtgaattgc
     3061 cgacaccgta tgtttccgtt tttcacgggt ataagcacac cgaacccgat caggtttaac
     3121 gaggaagaaa accgccgcgt ttatgagctt acgcagaagc agcgggctat ggagcgcaga
     3181 atgagacagc tcaagaaaga acaggcggcg gcaaaggaaa taggcgataa ggagacggtg
     3241 aagaagctca ataagaaaat ttccgagcaa agcacaaaga ttgatgaatt ctgcaagaaa
     3301 aacggcttga gacgagatca cagccgagag ctggtcaaag agcagatcat aaagaataat
     3361 gtacatcgca tgggaaatat ttattcttca ccactggaaa aagagtttgg aaagctcaaa
     3421 acaaaggaaa cagtaataac cgatgagcgt ctcaaacata taaaagaaag acatccggaa
     3481 gattatgaac tgtttaaaaa atatggatca gaagctataa aaaatcctga tannnnnnnn
     3541 nnatcctgat ataataattt ctgatttcga aaatgaaaat accgtattta tggtaaaacg
     3601 cttaggtgat acaaatataa acgttgttac aaagcttata ttaaagacgg atgattcaaa
     3661 gtttaaaaat tcggtgttga ccttttatag aataagagat aaaaacttaa taaaagcagc
     3721 aaaaaaagga aaaactcttt acattaagga ataattgtgc tattatattt atacaataac
     3781 taaggtattt tgaagtagag attgtgctgc tacgcacctt aatggttaaa agaaatgtgg
     3841 gaaagggcac acccaccaaa taccaagcaa aaaagccatc tgattttcag gtggcttttt
     3901 caatataata taatgattca agacatccga aagggtgtct tttttcatat acaccgctga
     3961 gcagtcggac ataaaaggct caatcacccc tgagcacggg acataaaagg cttaatactg
     4021 ccggagacac cggacataaa aacaaggagt atttatatgg aaaatcttga aatcctgaaa
     4081 ggcatcgtat ccgatgagac ctatgcggcg cttgaagccg aaacgaagga ctcgaaaatc
     4141 agacttggtg acttgtccac cgacaaatat gtcagcgccg acaagtacaa ggaccttgaa
     4201 aagcagttgg gtaaggtcaa cagcgcattg accgagaaaa caaccgaata cgataccctc
     4261 aaggaaaaag cgggcgacaa cgcagaattg aaggcagaaa tcgaacgatt gaaagcggaa
     4321 aacacaaaca cagtaaacag ccttaaggcc gattacgaaa agcagttgaa gcgttcaacc
     4381 gttatcaatg aaattcagaa caaatatcac ccgaaagacg ttaacgacat aatcccacac
     4441 atcgacatgg aaaaaatcag cgtcgaaaat gataatattg tcggtctgac cgagcagata
     4501 acgcctctta cggaaagtaa ggcatatctt ttcgacaacg aaccgcagcg aaataagagc
     4561 ggattgaagc atgacgacaa ggattatgac accgcaagac tcgaagcggc tttcggaata
     4621 acgaaaaagg agtaatgaaa catggcaaat acaattgcac ttgcaaagaa atattcaacg
     4681 atgcttgacc ttgtgtataa gcaggcatca ttaacaagcg tacttgacgg agcgcaggag
     4741 cttgtcaggg agggctataa tgccaacgaa atggtaattc ctatcctcga catgaacggt
     4801 cttgccgatt attcacgcaa cgacggctat gtaaacggcg atgtacagct cagatatgag
     4861 actgtaaaat gtaacttcga cagaggcaga atgttccaag ttgacgagct tgacaacatt
     4921 gaaacaggcg gcattgcctt gggcagattg ggcggcgaat ttgtgagaac aaaggtcgtt
     4981 cccgagcttg acgctttcag aatttccaag tatgcgagca aagagggcat ctcaaaggat
     5041 tatacgacct cgatcaccac gggcgcaaag gctatcgaag ctatcagaac agccaatgat
     5101 gccatggata acgacgaagt accttatgag ggaagaatcc ttctcgctac acccggtatt
     5161 atcggctcta tccaggatat ggatacaacc aagtcgagag agcttctcag atctttctca
     5221 ggtattatca agatgcccaa gaagagattt aacaataaga tcactcagct cagcggcaca
     5281 ggtgattata aagagggcgg atacagtgtt cccgaggacg caaatccgct taactttatg
     5341 atcattcacc cgtcggctct tattcagttt aacaagcttg taaagccgaa gatcatcagc
     5401 cccgaagcaa accagaacgc cgatgcatgg aagtacggct accgtcttgt ttctatcgcc
     5461 gatgtttatg caaataagct cgcaggcgta tatgcaagcg ttaacggtac cgcaccgacc
     5521 gccgccgggg acacgaacac ggagacacag ggtgatacat aatgtatcag ttctatgttg
     5581 aagagtatca cggaaataat atcgacaaaa ccacgtttga ccgcttatgc gtggaagcag
     5641 aagcttatgt tgacgctctg atcacgaacc ccgacaacct taaatatgaa cagattgaca
     5701 gaaagcgcag acttgccgtt tgcgccgctg ttgactgctt atatgcggag agggaaacag
     5761 cgaaacagag tataaaagcg agcgaaagcg tcggcgacca ttcggtatcg taccgcgata
     5821 caagaaaaac atccgatgaa tacagctcgg agcttacaca gaaagtaatg ctttatctgt
     5881 cgggcaccgg gctttgctat tcggctttga ggtgataatg tgtttccaaa cacaataaca
     5941 atctattccc attcctccca aaacctcaaa gatatatata ccnnnnnnnn nncacgtcgc
     6001 ccaagactac agccgaatac ggtaaatcct ggacggtaca caacggcgac cgcattgtaa
     6061 agggcgagca cggcgatatt gcttccttta aggatttaaa cggtgagcag gtcgtaacgg
     6121 taacgtcggt tgcggagcat atctgcggct cagaggttga taatatcacg atcgggggca
     6181 agtaagatgt ttacggttaa gattgatgta agcgacgctg caacaatttt gaagaaccac
     6241 ggattaaatc cgggcggaag agnnnnnnnn nngtgcaggc attttttaca agcgaagtaa
     6301 tgaaaaaagc tgatccgtat gtgccgtttt tggcaggcgc attgaaaaac tcggctcgtg
     6361 tgtcagagga taaatgtgct attatctatg atacgccgta cgcaagatac cattggttcg
     6421 gtaagctgat ggttgacccc gtgacgggaa aaggcgcatt ttacagcccg acttacggat
     6481 tttggagcag acccgacgta aacaaagaga aaaccgaccg ggaccttaaa tataacggtg
     6541 cgccattgag aggtcctcgg tgggtcgagc ggtgctggat aaacgaaaag gaaaacataa
     6601 tcaaatcaac ggaggcgttt atgagaaaat gacaataatc ga
//
LOCUS       DAWBSW010000114         6461 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2340_length_6461_cov_2.551046, whole genome shotgun sequence.
ACCESSION   DAWBSW010000114 DAWBSW010000000
VERSION     DAWBSW010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2340_length_6461_cov_2.551046"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4405..4439
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1845 a         1436 c         1475 g         1670 t
ORIGIN      
        1 ggttagtaca attccgaccg cacctttcac caaggcgtcc gaaaaaacca cgacagcaaa
       61 taatcagtaa ggggtaatca ttatgttgtt ttcacaggat ataggaattg atctcggaac
      121 atcaaacacc cttgttttcg ttaagggcaa gggtataatc attcgtgagc cttcggttgt
      181 tgccgttgac cagagattta ctccggtcaa ggttgttgcc gtcggttccg aggctaagga
      241 tatgatcggc agaactcccg gttctatcgt tgccctccgt cccctgcgcg acggcgttat
      301 tgccgacttt gacgcaacgg cggaaatgct taaaaaattt attcgcaagg ctatcacggc
      361 ttctccgctg agcaaaacga gagttatgat ctgcgttccg tccggcgtta ccgaggttga
      421 gcgcagagcc gttaacgatg ccgcaaaatc ggcaggcgca agatatgtca gccttattga
      481 ggaacccatg gcggcggcaa tcggcgccgg aattgacgtt gaaaagcccg aaggctcaat
      541 gatagtcgat atcggcggcg gtacggcaga ggttgccgtt gttgcactca gcgatattgt
      601 aacctctcgt tcggtcaagg gcgcaggcga cagctatgac gaggcaatta tcaattatgt
      661 cagaaaaaaa tataaccttc ttatcggcga aagaacggcc gaggatataa aaattcagat
      721 cggctccgcc tatgaatacg agggcgaggg cgcaatcaac gttaagggca gaaacctcat
      781 tgacggcctt ccgaaggacg ttgaggttac agctgctgaa atcagagagg ctctcaagga
      841 atcggtctat caaatcatcg acgcaatcaa aatcactctt gagaacactc cgcccgagct
      901 cgccgcagat attattgaca acggcatcac cctcacaggc ggcggcgcac agctcagagg
      961 tcttgacaag ctcatcgagc aggaggtaaa aatccccgtt acggttgcgg acaatccgct
     1021 tgactgcgtt gttgagggaa cgggcatttg ccttgaaaaa cgtcgctgat gaaaaaactt
     1081 tctacaaatc aaggaggtga agaggaatga aaaaaattat cagaaaccgt gcgtttaagg
     1141 cgcttgtctg cgttattgtc gccctttcgc tgggcgctgt tattgcagca tacacgcata
     1201 atagctcaac tccgctttcg tcagccacaa gtacggtgct tcatcctctt cagcgctttt
     1261 cttcatatct aagctataaa ttttccaact ttaacgacag ctttaagtcg tcaaagactc
     1321 tccaggagga aaattcaaag ctcaaagaac agattgaaga atatcagaat aaaattattg
     1381 attataacaa cataaaaaag aagctcggta cttatgagga ctttctcgga gttaaagagg
     1441 aaaatcccga cttcaaattt cagaacgcaa aaataatttc aaacgatttt gaaaatccgt
     1501 tttcttcatt cacccttaac aagggcacga cggcaggcgt taagatcaat gaccctgtta
     1561 tttacggcaa aaatcttgtc ggcgtcgttg caagcgtcgg tccgacttca tgcacggtct
     1621 ataccctttc aaatcccaag atgaacgtca gcgtttacga aacctacacg ggagaaatcg
     1681 gctatgttac gggtcttggc aagaccgagg acagagttta ctgcaagctc ccgggtctta
     1741 aatcggattc gtccgtttca ccgagcggaa ttatctgcac aacgggaaac ggcggtgttt
     1801 atcccaagga tttgattgtc ggcacggtag ttaaacttga aaactccaag gaggataatt
     1861 cctcctttgc aacaatcagg tcggctgtca atatcgagga gctttccgag gttttcatca
     1921 tcaccgagtt tgaaggtcag ggcgttataa ccatcacggg tgactgatat gaattatatt
     1981 gaagagctta aaaaaaatga cagtataaaa agaagaatac ttctcgccgt attggttctc
     2041 gtcacgttta tttttcagac aacgaacgga tttttcccct ctcccttcgg aatacatgct
     2101 tgcctgctgg ttccagcaac ggtttgcatt gccatgtttg agcgtgaatt ttccgggctg
     2161 ttttttggcc ttgccgcagg aatgatgctt gaagccgcca gccccgacag cgtgtgcttc
     2221 tattcggtta tgtttaccct tataggcttc gtctccggta tgctcataac ctaccttatg
     2281 agaaacaatc ttgtaactgc gctcatattt acatttgtgt ttacattgct tctcaattcg
     2341 ctttattgct tatttttcta tgtctttgac ggaatagaaa tcacttttgt tttctatctt
     2401 aaatattatg ttttttctgc cgtttacaca acgcttttta caccgatttt ctatctgatt
     2461 gtccgtctca cggtcaaaaa attcaaataa aaccaaaaag tttttaaaaa aaggagtgaa
     2521 aaaatgagac ttgcgcgtga agagaaaaaa ggaagattta ttgcaatgat aacgattttc
     2581 gtctgtctta cggcggtttt cattttcacc cttgcaaaaa gtcagataat cgacggtaaa
     2641 aaagacacgg cgtcgaacac cgcctcggtt gaatcaacag aggtcaaagc aacaagaggt
     2701 cagatccttg accgcaacgg taaggttatc gtcggcaacc gtcagggtaa tgatgttgtt
     2761 ttcaacgctg cggattttcc cgaatattcc aagcaggagg agcgtaacaa gcttattaaa
     2821 tccctcattg atctttttga aaagaacaaa gttaaatgga acgatgatat gcccatcgtc
     2881 ctcgattcaa acggaaatta tcagtttgcc gaggacaggg aaaaagatat cgaaaccatg
     2941 aaatcccgag atattcttcg tcttaataag tatgccacgg cagacgattg catgaacgag
     3001 cttgtcgaca gatataagct tgagagctat tcaaaggcag acagaagaaa aattgcttcg
     3061 gtctgttatc aaatgaagat taacaatttc aattcggcaa atccctatgt ttttgcaacc
     3121 gatgtcacag atcaggttgc ttcggtaatc aaggaaaaca gccgttatta taagggtgtt
     3181 gatattcaga taggcaacta ccgtgaatac accgacggta ctcttgctcc gcacataatc
     3241 ggcgtaacag gcgttatcag tgccgaggaa tacgccgagc tgaaaaaaga cggctacgga
     3301 atggatgata ttgtcggaaa aacaggtatt gaacagctct ttgaaaaaca gcttaagggc
     3361 aaaaacggaa cgaagcttgt caccaccgct cttgacggca cgcagacgac cgttactaag
     3421 gggctggaaa acggcgataa tgtttttctt accctcgatg cagggcttca aaaggtcact
     3481 caggacgctt tggcggcaaa gatcaactcc cttgacagcg caaacaaagg cggcggcgca
     3541 gcagtcgtca tgaactgtaa gacgggagag attctttcaa ttgccaccta cccgtcctac
     3601 gacctcaaca catactataa gaactattca aagcttgcga aggattccaa ctcccctctt
     3661 tataaccgtg cgcttctgag cacttatgct ccgggttcaa ccgccaaggt ttcaacagct
     3721 atcggtattc ttgaggaggg cgttgccgat gaaaattcaa cgattgaatg tacaggaacg
     3781 tataagtacg gtcttgtttg taataacaac ccggaaacga ccacaattga tataagaacg
     3841 gcaattcagg attcttgtaa catatatttc taccatttcg gcggcgaact gctcggaatt
     3901 gaaaagatga atatgtaccg tgaaatgctc ggactcggtc aacctaccgg catagagctt
     3961 gacgaaaaca caggcgtcct tgactcccac agctatcgtg cctcaatcgg ccagacatgg
     4021 caaaccggct tctcgctcca gtcggcaatc ggtcaggccg gcaacctctt cactcccctt
     4081 cagctttgca actatgtttc aactatcgca aacggcggaa cacgctatgc cgcacacata
     4141 gttaaagcgg tttactccgc agacaattca accattatcg aagaaaagca gcctgaggtt
     4201 ctttgcgaaa caggcttcaa aaagaagaat attcaaataa tcaaggaagg tatgcgccgg
     4261 gttgcgaccg taacccttgc cggaagcttt gacgattctg ttgttgaggt tgcctgtaaa
     4321 acaggaactt caacccttga gaaaacaatt aacggaaaaa ccggaattta tacaaacgga
     4381 tttcttatta caatggcacc gtatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4441 acaggaagtg atcaaatggc aagaaaaatt gttttggctt cgggcaaagg cggagtggga
     4501 aaatcttcac tgaccgccgg cattgccatt gagctttgct ccatgggtca gaacgttttg
     4561 gttgttgatt ttgatatcgg aatggggtgc cttgacctta ttcttgcttc tgatgacaca
     4621 ggaattttta actggggcga cgtgatttca ggctcatgcg aacctgctca ggcgcttcgt
     4681 tcaacagtcg gtccgtctct gctgaccgct ccgacctatt ttagcgacga atataatgaa
     4741 aaaagtgtca tggaaatgat tgaaaactat gatgacaact ttgattatat tcttatggac
     4801 gctcctgcgg gtgtcagcgg tggttttctt ctcgctgcgg catgcgccga tgaaggaata
     4861 atcgtttcaa ctgccgacga tgtctgtgtt cgtgtcggtt cctttgccgg cgacaagctt
     4921 tcaacgctcg gtgtcggaga aatcaagctt attattaacc gttttaataa aaaacaaacc
     4981 acacggggac attatctcaa tatcgacgag gttatcgacg caacgtcact ccagcttatc
     5041 ggagttgttc ccgaggacag caaaatttcc tattgctccg ttacaggcat gagaatgctg
     5101 gataactcaa aggcaaagga agcctttaag cgcattgcaa tgcgactgga aggctttaat
     5161 atcaatctta aattgtgagg tcacgaaatg agtgatttat cagcaatcgc cgccatatcc
     5221 acaccgttgg gtaccggcgg cgttggaatt atcagaatat cgggaaaaaa tgcaactgaa
     5281 attgccgaca gaatttttgt ttccgtaaac ggcaaaaagc tcagttcttc caagggctac
     5341 agagcttatt ttggcaggat ttttgacggt gaaacagccg ttgatgaggt cgtttgtctt
     5401 gtttttcgtg cgccgcacag ctacacaggc gaggatgtcg ttgagataaa ttgccacggc
     5461 ggtgttgttc ttttgaaaaa aatactccgt ctcgttctgc aaaacggggc tcaggcggct
     5521 gcaccgggcg aattcacaaa aagagctttt cttaacggta agcttgacct ttccgaagcc
     5581 gaaagcgtta tgactcttat atccgctcaa ggcgaacagg gtgcaaacgc cgccttcaac
     5641 cagcttgaag gcagtctcag ccgtaaaatt gaaaaaatca actcctctct cctctctctt
     5701 gcagcccata ttgccgcttg ggttgattat cccgatgacg agattgaaga attaggcaat
     5761 aacgagcttt acagtacaat atataatgct catttggaat tatgtgcttt gttaagcaac
     5821 tttgacagcg gaatggcagt aacaaacgga gtagaagctg caattgtcgg aaaaccaaat
     5881 gttggaaaat ccactcttat gaatctcttg acgggctatg accgatctat cgttacggaa
     5941 attgaaggca caacaagaga cattgttgaa gaaacggtta acctcaacgg ctgcattctc
     6001 agaatttcgg atacggcagg aatgagagag acaggcgaca tagttgaaaa gctcggagtt
     6061 gaacgaagca gaaagaagct tgaacgcgcc gctattgtat tcgctgtttt tgatctctca
     6121 aaaccgcttt ccgacgagga taaggaactt attgatgagt gcaaagacaa aaatgttata
     6181 cctattgtaa ataaaacaga ccttgaacct cgtcttgatg ttgattacat taaaaatatg
     6241 cttggctctc cactgttcat ctctgccaaa agcggcgacg gctacaatga actttgcgac
     6301 cgagttgccg aattgatggg aactaagaat ttcgatacaa catcggcaat gcttgtcaac
     6361 gaacgacagc gcatttgctg tcaaaaagct tccgatgcgt taaaagacgc tcttgaagct
     6421 ttgaatattg gcctcactcc cgatgctatc ggcgtttgca t
//
LOCUS       DAWBSW010000115         6375 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2363_length_6375_cov_2.778006, whole genome shotgun sequence.
ACCESSION   DAWBSW010000115 DAWBSW010000000
VERSION     DAWBSW010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2363_length_6375_cov_2.778006"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1696 a         1550 c         1379 g         1750 t
ORIGIN      
        1 cctttgatct tattgtaacg agggaaaccc ttacgtttga aacgctgaaa aatcacggga
       61 tagaaaaaaa taccgtgctt tatcccgatc ccgcttttac ccttgatatt gatgaaaaaa
      121 caggcaaaga aattgaagtt aagccgaata cgctcggatt taacatcagc tcgcttgtcg
      181 gtaaatactc cgctaaaggc gcaagcatag atgatatttc gacggaattt ctccgatata
      241 ttcttgataa cagcgacaag aatatacttt taattccgca tgttacgaaa gaaaatgacg
      301 gtgatcagct tattctctcc gggattgccg aacagctcgg ctcggacaga gtttccgttg
      361 tcccctcaac gtttactgcg tctcaataca agagtataat ctcacgctgc gatatgttca
      421 tcggtgcaag aactcatgca acaattgccg cttacagcac atgtgttccg acacttgtta
      481 taggatacag cgtcaaatca agaggcattg ccaaagacat tttcggaacg gatgacggac
      541 ttgttgtccc tgtttttgaa attgacagtg cagaaaaact cataaaagca tatgaggatt
      601 tttcacgcaa aaaggaaata taccgcaagc agcttgaaaa aattatgcca agctacattg
      661 aaaaggcacg aagctcgatt aacgaacttt tcagaatata aaattatccc gactctcatt
      721 tgaaagtcgg gatttttcat gtgcaaaagc aggtaattgc cgaggcaact acctgcttaa
      781 tattatttat tcggagaaat tattcctctg tgccgtaaag cttaacaagc ttctggagat
      841 gtgcataacg atcctttgcg ccgtcctctg ccttctcgaa gagcttttct gctctctccg
      901 ggaacgaacg tgtaagagcg gagtaacgag cctcgttcat gaggaatgca cggtaaccgt
      961 ctgttgcggg aaccttagaa tcaactgtga acgggttctt gccctctgcc ttaagagacg
     1021 gattgtaacg gaacatattc cagtaaccgc aatcaacagc tctcttcatc tcagcctgac
     1081 agttggtcat gccgcccttg atgctgtgca tctcacaggg agcgtaaccg ataacaagtg
     1141 acggacccgg ataagcctca gcctcctgga gagccttaag tgtctggttc tggtctgcac
     1201 ccatagcgat ctgagcaaca tagatgtagc catatgacat cgcaatctcg gcaagactct
     1261 tcttagcgat tgccttacct gctgctgcga actgagcaac ctgaccgatc tgagaagcct
     1321 tggaagcctg tccgcctgtg ttggagtaaa cctcggtatc gaataccata acgtttacat
     1381 cctcaccgga agcaagaaca tggtcaagac cgccgaaacc gatatcgtat gcccatccgt
     1441 caccgccgaa gatccatacg gacttcttgg agaggtaatc cttctgagcg aggatttcct
     1501 tagcgtcatc gcagccgcac ttttcaagct cagctacaag agcatctgtt gcagcgccgt
     1561 tcttctcgcc gtcatttact gttgcaaggt aagcatctgc ggcagccttg atctctgcgc
     1621 tgacgccttc tttagctgca agagccgtaa ccttctcaat aaggttattt ctgagagcct
     1681 tctggccgag gtacataccg tagccgtgct ctgcgttatc ctcaaagagt gagtttgccc
     1741 atgccgggcc cttgccgttc ttatctgttg tgtacggtga tgtacctgcc gggccgcccc
     1801 aaattgagga gcatcctgtt gcgttggaga tgtacattct gtcaccgaaa agctgtgtaa
     1861 cgagacgagc atatgaagtc tcggcacaac ctgcacaaga tccggagaac tcaaggagag
     1921 gcttcttata ctggctggag ataacgttga tcttagccgt aagatccttc ttctcttcaa
     1981 catgagctac gcagtaatcg aatgctgcct gctgctcatc ctggctctca cgggatacca
     2041 tcttaagaga cttagtcggg caaacgccga tacatacgcc gcaacccata caatccatcg
     2101 gggaaattgc aagagtatat gtgtagtttg tctttactac cggcttagga gcgaactttg
     2161 taactgccgg agcagctgcc ttctcctcat cattgagagc gaaaggtctg attgtagcat
     2221 gcggacaaac atatgcgcac ttattacact ttgcgcaggt ctcagcgttc cactcgggaa
     2281 ccataactgc aacgccgcgc ttctcatatg ccgatgcacc ctgctcgaat gtaccgtcaa
     2341 catgatttga gaacttaccg actgaaagct cataaccgtc catatgacct acaggcttca
     2401 tgatgctctc aacctgctca acgagctttg ccggaccttc ggtcttaacc tcagactcag
     2461 cgtcaactgc gtttgcccag tcagccggaa cgtcgatctt aacgaatgct gttgcacctg
     2521 cgtcaatagc cttctcgttc atctcaacga tttctttacc cttcttcatg aacttctgtg
     2581 ccttttcctt catgtagccg atagcctcgt caacaggaag aacctttgca agagcaaaga
     2641 aagcggactg aagaactgta tttgtacgct tgccaaggcc gatttttgcg gcaatgtcaa
     2701 tagcgttaac tgtataaagc tgaatattgt tctttgcgat gtactgcttg gtttctgcat
     2761 tcatcttctc agcaagttcg gcggcatccc actggcagtt gataaggaat actccgcccg
     2821 gcttaacgtc ctgaaccatc ttgtaaccct tttcaatata tgacgggttg tggcaggcaa
     2881 cgaagtcagc cttgtttaca tagtaggggc tcttaatcgg ctcatcaccg aaacgaagat
     2941 gggagatagt aataccgccg gtcttctttg agtcgtactg gaaatatgcc tgagcatact
     3001 tgtctgtgtg gtcaccgatg atcttaatgg agtccttgtt tgcgccgact gtaccgtcac
     3061 cgccgagacc ccagaattta cactcgattg tgctctcggc tgctgtattc ggagccggct
     3121 tctcttcaag tgaaagataa gtaacatcat cgttgatacc gattgtgaag tgagccttgg
     3181 gagcgtcctt cttgagctcg tcatatacag cgaatacgct tgacggcggt gtatccttag
     3241 aaccgagacc gtaacggccg ccgataacct tgatgccttc cttgcctgct gcattgagag
     3301 cggcaacaac atcgaggaag aggggctcac cgattgagcc gggctccttg gttctgtcaa
     3361 gaaccgcaag cttcttgcat gaagccggaa tagcttcaat gaacttctca accgagaaag
     3421 gtctgtaaag acgaacctta ataagaccaa ccttctcacc cttagcggta agatagtcga
     3481 taacctcttc tgcaacgtcg cagattgaac ccatagcaac gattacgttc tctgcgtcct
     3541 cagcgccgta gtagttgaag agctgatagt ttgtgccgag ctttgcgtta accttgccca
     3601 tgtacttctc gacaacagcc ggaactgcgt catagtactt gttgcaagcc tcacgatgct
     3661 ggaagaagat atcatcgttc tcgtgagaac cgcgcattac gggatgctcg gggttaagcg
     3721 ctctgtgtct gaatgcagca actgcatcca tgtcgcacat ttctttaaga tcatcgtaat
     3781 cccaaacttc gatcttctga atctcgtgag atgtacggaa accgtcgaag aagttgatga
     3841 acggaactct gccttcaata gctgcaagat gagcgactgc accgagatcc ataacttcct
     3901 gcggatttgt ttctgcaagc attgcgaaac ctgtctgacg gcaggcataa acgtccgaat
     3961 ggtcgccgaa aatgttgagg gcgtgagacg caacgcaacg agccgataca tggaatacgc
     4021 agggaagaag ctcacctgcg atcttataca tattggggat catgaggaga agaccctgtg
     4081 acgccgtata agtcgttgtg agagcacctg ctgcaaggga gccgtgaact gtacccgaag
     4141 cacctgcctc ggactgcatc tcagcaacct ttactgtcgt gccgaaaata ttcttacgac
     4201 cctgagcaga ccactgatca acgtagtcgg ccatcgggct tgacggggtg ataggataga
     4261 taccggcaac ttccgtgaac gcataagata cgtgagcggc agcggtatta ccatccatgg
     4321 ttaacttttt tcttgccatt ctttgttcct ccttgtaaag ttaatagcaa tcttattacc
     4381 acccataata ataacacttt atttttgata tgtaaacatt attaagagat ttttttcaac
     4441 tttttattcc cgtttttgcc ataaaatgtt tcaaaaagcg gctctcggac agtttttttc
     4501 attttttcgg atatttattc gtttttgttt gatttaataa agaaataacg aagctgccgc
     4561 caaaagcaac agctccgttg ttttttacat tatagcatat tccgcaatgc gcttgacaca
     4621 ttcaccgtcg catgcgccca tgaatttttc tctgaatgca ggaatcctgt caactcggaa
     4681 atcaccggcc ttcacggctt caattatctc gtcgttgttt ataactatcc tgcccggcac
     4741 aaagctcttg taatcaaaat aaaaactcct gtcgtgctca tattcctcaa ggtcatacgc
     4801 ataaaaaagc atgggcttat caagtaaagc atattcaaaa atcagtgagg aataatcggc
     4861 aatcagaata tccgccgcac agagtgcggt ttcgattttt aagctctttg aaccgtcgaa
     4921 aataaagtca ccgtacttct ccttttcttc ttcggtcaag ccgaatttct tcgctgtgaa
     4981 cggatgaagc ttcatcacaa gggcgtattc gtcaccgagc gcagctttga gcttttcgta
     5041 atcgagcgga ttttcgttat aagccgcagt cggcgaattt cctctgaatg tcggagcgta
     5101 aagtatgatt tttctttcgc cgatttcggg aaaaattttc aacatctcct cacgctgaga
     5161 agatacaaag ctcttatcaa aataaacgtc ggttctcggt gttccgaaag ccttgattct
     5221 gcgctccgag cagcgaaaag cctctgcata gcacggaata accgcctcgg acgagacaaa
     5281 aacatcggta tatgtattgt gcatcggaaa tctgagcata tcctcacggc ttccgcccca
     5341 agcgagatca agcgttgaat aaccccactt tttgaacgct ccgcaggcgt gccaaagctg
     5401 aattaccttt gtaccctttt gcggctgatg cgcatagagc gggtcaaaat tgtcggtcag
     5461 aaaaacgcat ttgcactgtg cataatattt ttggaaagtt attccgaaca gaaatctttt
     5521 taatgagctt ttcggagaat tcataattcg gcaatcatag cctttgtctt taagaaagtc
     5581 atatatccca accatgttat cgggaagagt gtcataattc tgattgtaag caaaaagtat
     5641 aagcttttcg tttatcggct tctttgcaaa aaaattaaac gccatcggcc aaagccaaaa
     5701 gaaaagaatt cttcttctga gtaagacaag ttttttcttc aattcgttca tggaatccac
     5761 ctttcggttt attatggttg ataaactaac cgctgagaag acaaagagac tgtcacccgg
     5821 acagtctctg aatcatcatc ttaccattaa acggcacgtg taaccttacc tgaacgcaag
     5881 caacgtgtgc aagcatatac cttgcgcgga gaaccgttaa cgattgcctt aacacgctta
     5941 acgttcggct tccatgttct gtttgaacgt ctgtgtgagt gggaaacctt gataccgaaa
     6001 gcaacatctt taccgcagaa ttcgcatttt gccatttgtc ggcacctcct ttaactacga
     6061 atactaaaca acatttcata tactaacaga tttatgcgaa aaaatcaaga cttttttgga
     6121 aataattttt tatatcaagt taaaaatcgc aaaatccgcc tttatactat gaattttttc
     6181 atcaagtcgg ggccgaaatc aatataatta cccgtcgcct tggctgactc ctcatttatt
     6241 gactcaactc cgctgtcctt ctcatcggat ttctgataaa tcatgtaggg gacgggatcg
     6301 ctcgcatgtg tcttggtaac gatcggtgtc ggatgatcgg gaagaatcat aatcttataa
     6361 tcctctccta tctcc
//
LOCUS       DAWBSW010000116         6247 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2413_length_6247_cov_2.257106, whole genome shotgun sequence.
ACCESSION   DAWBSW010000116 DAWBSW010000000
VERSION     DAWBSW010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_6247_cov_2.257106"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1869..1878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1818 a         1405 c         1312 g         1702 t
ORIGIN      
        1 gtattcgctc aaatccaagg gagtcgacag ggactcggta attaagctca tcaacaagat
       61 atatgaggag gaataataaa tgattgaaat aaacaacata acgaaaacct tcgatgatta
      121 tacggcaatt aagaacctct ctttcacagt caacgaatcc tcgctttacg ggcttgtcgg
      181 ctacaacgga gcaggtaaaa caactcttct gaaaatcatc gcaggcgttt atctccccga
      241 ctgcggcgac gttttgctca acggcaaaag tctttttgag catccggagc ttaaaacaga
      301 ccttttctat gttcccgacg atttgtattt cggtctcggc gcttcgatga attccatggc
      361 aaagttttac atgggctttc acccgaactt ttgcattgat acattcaaaa agctgacagg
      421 tcttttcggc cttgacccga agaaaaagct tatcggtttt tcaaagggta tgcagcgtca
      481 ggccgaaatg atactcggtc tctcctgcct gccgaaggta attcttctcg acgagtcctt
      541 tgacggactt gacccggcaa agagaaatct gacaaaaaag attttgcttg actacatggc
      601 agaaaaggaa tgttcgataa tcatttcctc tcataacctc catgagcttg ccgatatgtg
      661 cgaccacatt gcacttatca acggtcagac gattgcaata aactgcgccg ttgacgacat
      721 gaacggcaca cgctgcaaat tccgccttgc cttcaagaac gaggttgagc gcgaagcatt
      781 cggtgacctc aaaatcaaga atttcaaaaa agacggcaag atagtaacat ttacggtctc
      841 ggaaaatgtc gaaaaagctc aggaaattat caattcgctt gaccccgtgc tttgcgaacg
      901 cttccctctt tcacttgaag agattttcct tgaggagatg gaggatacgg attatgactt
      961 tgaaaaaatc ttctcgtgat ccctttgcgt caatgttttg gaactctgtc aaaaagacgc
     1021 gttttgttcc gatcgtgacg cttgttgtgt tgcttctcac cacactgctc gaatttgttt
     1081 ttgattatat aaacggttat tatgattgga accaaaaacg atacggacat ttttgggatt
     1141 ccattcttga cggaatactc cttgctgttc cgatcatagt tattttcgga gtcatctcgg
     1201 gaaccgtttt attcagattt gtacaaagca agaagcaagt taacgtcatc ttttcgctcg
     1261 gactcagccg caggaaaatt ttccttgcaa aatacctcgg cggaattgtt ccccttgcgg
     1321 ctgcgcttgc cttttcggca acagtcgaaa ttcttgcaga cgttatttcc ggctgtatcg
     1381 tcaaatttcc gacaatacac ttggcgctga tttcggtagc cggtatactc ggaacatata
     1441 cattcgcatt taccgttgcg gctgtatcaa tggctttttc gggaaacatt gtcgaagcgg
     1501 gaattttttc ggtcatactt gcgatttttc cgaatttttc aggtattttc tttggccata
     1561 tgcgcagcat ctttacccat ggaggtgtcg aagcctatgg caaagactgg aactttttca
     1621 gccctttttc ggcattgctc tttgtgcaaa caggcgaagg ctacgaaaaa aactatcttc
     1681 tccgtattct ctatacgagc gagaaattga ccgccaccga ctggtcgggt acgatcactt
     1741 gttttgttct ttctgcaatt atttttgcaa ttgcttattg ggcgttcccg aagcgcaaaa
     1801 acgagaccag cggttatttc ggcagagcga agggtctcaa cgaaatctgc ggcgcaatga
     1861 cggaatttnn nnnnnnnnaa cgattgtttc cttaaatctg acggaaaatc acagtgcaat
     1921 tatcgcgttc atttcattca ttttaacttt tatacttgtg tatttggttt tcaaattcct
     1981 tttcacatac aagcgaatga aaaatatgaa gcaatcggta aaaagaattg ccgcatatgt
     2041 tgccgctttc gctttaattg ctgttatttt ttcaacggga cttttcggtt attcctcata
     2101 tgttccgaac gctaaaaagg tcgaatcaat tgaaatttct ttagaaattt ccaatccgta
     2161 cgacacaatc aattacagca cggatattca cggcgatgag gtctacggct actctccgat
     2221 gataagcaac ggcttttcaa gccaggttga attatccgac gttatttttt ggggcggaac
     2281 agcaaacttc ggatctttta atctcgactg tacggttgaa aaagacaatg agattaaaaa
     2341 agttattgat gttcacaaag ccattgccaa agagggcaag atcaaagcca atgcggacaa
     2401 cgcttgcggc tacgttatta tgataagcta caagcttcgc aacggcagaa cggtagaacg
     2461 ccgttacagc accgtcagca cggaaagtgc aatgaaaatg ctcggcctca gcgaccttga
     2521 aggatttaaa gacgaaatat ctgaatattt ctattatcat tcccaatcaa tggaaaagct
     2581 cgggccttcg gactatctcg acggtttctg tctgctgtcg aaaaatctca agagctgcaa
     2641 gtatgttgac ggactgaaat acgactttat ggaagccatt cttgccgacc ttgaaagaca
     2701 aagtgcacag cagatttatt ttcacagtcc cgaggatgag cttggagtta tctatttccc
     2761 gcttaacgat tcggtttcat caatcctcaa cctccgggat aacgagataa tcggcagcga
     2821 cggagtaatt atcaatgccg atacaaacga aattacgggc actgtgaagg aacgccgtgc
     2881 cggatttccg aatcttcccg atacgagtat ctcaatctcg ggagatagat gcatagttgt
     2941 cacgaaggat atgacaaata taattaaata tctcactgac aaaaatctta tgcaatactt
     3001 taaatccaac gtcactgcca atgacgtaaa caaaattaag attgcaacaa gagccgaaac
     3061 ggtcggcaag aaaaactccg aaatgcttcc gctctttgct gccggctatg cctgcgccga
     3121 tgaggtcaag tcgaacgaca ccatgagcag acaggcaagc cacgactttg caaaccacgt
     3181 cggcggatca atcaaggaca aagctacgat tcaaaccgta cttgacaata ccctgcttta
     3241 cggctttaac gataacagtg accgcattgt tgaggtaaca tacaacgacg gctcgattgc
     3301 tacctacgcc ttagaatcgg acgtttacaa caagctgaat attaaatagg agggaacaat
     3361 ctatgaaata taaacataca gcggccggac ctttctcagc gctgttacga aactctttta
     3421 taaaaacctg tttcgttccg ataatcacct tttttgcgct gttcatgtca acgctattta
     3481 actatatcaa cataaaagca ttccatcgct ttggcggttc accgtttgta aaagacaaat
     3541 ttttcggatt tttctggagc aatgtcagtg acagcatcct tcttgcggaa gtgctgatta
     3601 tcctcggcgg cgttttcgcc ggaatagtcc tcttttcatt tgcacagagc aaaaagcact
     3661 gcaatgtcat cttttcgctc ggcataagca gaagaaaaat cttcctggca aaatatctcg
     3721 gcggacttct ccccttctgc gctgccgtca ttatagccgc attttttgag cttgtgtctg
     3781 tattcggcgt cggttacacg ccgaacgcac cgataatcaa gatggctgtt tactgggtag
     3841 tctgctttat cggaatatac gccgtctcat tcactataac ctcggcggta atcgcctata
     3901 ccggaaatat tgcggagggc ttggtattca ccgccataat agccgtattt cctatggctg
     3961 ccggtgtgct cttcagcgaa atgagaggac tttacactgt cggtggaata ggcgtatatg
     4021 agggtgactg gaacttcttc agtccttaca tttatatgtt cagatttttc agtccgggcg
     4081 aatatggcgc tcaggagcaa tacagcgaag gcttgaacat ggtctttagc agctatcagc
     4141 tttattttac attcaacgaa agcggtgcac cgctttataa tctcacaatt tccgattact
     4201 cgggtgcaat cacggacttt gtctatgccg caataatttt tgcaattgct tttctcgctt
     4261 ttccaaagcg cagaaacgaa atcagcggtt ctttcggaag agcaaagggc atcaacgaga
     4321 tctgtgctgt attttcggga atatatgtca tgactttcgg aatgtatgct gttgtcgaag
     4381 gcacactcgg aatagaaaga ggaaacatgc tcaccttcct ctactcaatc ggcttcttca
     4441 ttgtagcgta tttcgttttc aaaatgatct tcgctcataa gcgtaagctg atattgaaat
     4501 ccatgctcaa aagaataccg tgctatatcg cagcaatagc gatagttacc acggttttct
     4561 actccggact tttgggctat gcttcaagaa tacccgacgc cgaggacatt gattcaatcg
     4621 agttttcttc tacaatctcc aacccttaca gcagcgttaa cgataacgat gccctccatc
     4681 gccgtccgat ttacggatat gcggtaatga agacctactc gggctacaca agcaccggcg
     4741 gctttgatcg gttcttttac ggcgaaggct atcaggatat atattacccc aaatactccg
     4801 ttgacgatga gagcatgatt cacaggctta ttgaaattca caggagcttt gttcaaaagg
     4861 gacatatcaa gagcacggct tcggacacct gcgcaatgaa cttccagata gtttatacgc
     4921 tcaaaaacgg caaaacaatc aagcgttact acagcgtaac aacggaagaa aacgcaaaga
     4981 gaatcatggg acttagcgat acaattgtcg ccaagagcgg aattgaagaa tattttgaaa
     5041 ccaattccga cgattactat gcaagcgata gctatttaag caaagtatct gccgaattga
     5101 atcccggaaa ctatttctat ctctattcaa agtatcttaa agacagttac cgctgcggca
     5161 taataaccga tgagctgaaa aaagccgttc tgaaagatct cgaaaaccag acggcgaacg
     5221 atattttctt ccacaagccg gaggatgagc tgggcgttat aagctttgga atcggtgata
     5281 atttcataga cgatttgccc gataattatt gctgcaacga atatggggat attattgaca
     5341 gatatacagg cgaaattgta aatgataaca ctctgcaaac agaacaggcg aaggaatcaa
     5401 tgaagcttac cgacctgacg attggcgcaa tggacaacac atctaaatca ttcgttataa
     5461 caaagaccat gacgaacacg ataaagtatc tcaccgacaa cggatacatg aagtgtcttg
     5521 agtcaaaaat cacggcaagc gacgtcaaaa gcatgaagct ttgcacaaaa tcggagtcca
     5581 tcaacaaaac aaatgccgat atgctgccgc ttttctcggg cggatataac agcgttgccg
     5641 atataaagag ctttgattcc gacatggatt tatattacgc caattataat caattgaatt
     5701 cccattatat cgagcataat gttcccaacg agataaccag caaatcaacc atccaaaagg
     5761 ttcttgacaa ctgcttcctt tacggctact gcggcaacga ttaccgaatt gccgaaataa
     5821 ccttcaacga cggctcaatc gcaacctatt gcattaccgg cgaggtttac gaaaagctga
     5881 tgaagtgagt tttgttgcac aaacattccc gcctccctta ccggtgaggc ggggcagcag
     5941 aattctatct cattattaag caaaataagc ctcagccacc aaaagtttaa cctatcatta
     6001 ttaaggtgat aaaatgaagt tcaaaatcaa gaagaaacaa agaaatatca tcattgccgc
     6061 tgttatttta acggctgtca taattgctgt ctgcgtacga agcggagttt ttaaacccaa
     6121 atacacctac tccggtgacc cgtacgtttg ccttgacgca ggacacggtg cctatgacgt
     6181 aggcgcagca aacggcaacc gctacgaaaa agacgatgac atcaagctga cgctgaaaat
     6241 cagagaa
//
LOCUS       DAWBSW010000117         6197 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2430_length_6197_cov_2.540703, whole genome shotgun sequence.
ACCESSION   DAWBSW010000117 DAWBSW010000000
VERSION     DAWBSW010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_6197_cov_2.540703"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    232..241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    779..883
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1769 a         1304 c         1526 g         1483 t
ORIGIN      
        1 attctctatc ttgcgacgga gggcttgtac gctgagataa cctcatcaaa ctcgggcata
       61 atcgacagaa ttcagcgtga gaatatcatg gttgcgggac cgacgacgat aatcgcactg
      121 ctcaattcgc tctcaatggg cttcaaggcg gttgcgataa acgaaaaggc gaacgaggtc
      181 agaaagcttc tggcggcggc gaaggtgcag tacgataaat tcggaattgt cnnnnnnnnn
      241 ngatttctgg catctcaaaa gcatcgagag actcggactt ccggaaatca tggtctgtga
      301 cggacctcac ggtctgagaa aacagaacgc agagaacaag aaagtcggaa tcggcaacag
      361 ctatccggcg acctgttttc cgactgcggt tactactgcc tgctcgtggg acagagacct
      421 tatttacaag atgggtcagg cactcgcaga ggaatgcttg caacacggcg tgagcgttct
      481 gctcggaccc ggtgtgaaca tgaagcgttc acctctctgc ggcagaaact ttgagtattt
      541 ctcggaggat cccgagcttg caggcgagat gggcgctgcc ttcatcgcag gtgtccagtc
      601 caaaggcatc ggcacttctc tcaagcattt cgcaggcaac agtcaggaaa tgaagcgaat
      661 gacctcaaac agcatcattg acgagcgtgc actcagagag atatatctca gagcttttga
      721 aaaagcagtt aaaaagagtc agccgtggac ggtaatgaac gcatacaatc tcctcaacnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcgggc ggagttaacg
      901 acgcaaaaat cgttgaagcc gtcaagtgcg gagttatcga cgaaacgacg cttgacgaaa
      961 gagtagataa gcttattgat atcattctca agggcgcaga taataaaaag ccaggttata
     1021 aatttgatgt taaggctcac aaccttcttt caaggcaaat tgccgagcag tcaatggttc
     1081 ttcttaaaaa tgacggtaat attcttccgc tcaagcgtgt tgagggcgag tatgttgcgg
     1141 ttatcggtgc atttgccgac aacccgagat atcagggcgc aggcagctct ataatcaatc
     1201 cgactatgat cgacagcggc agacgcgcat tcaataattc acctatcagc gttaagtttg
     1261 ccgacggcta tgacagaagc ggcaagagaa agaatgagga tgcttatata acagaggctt
     1321 gcaaccttgc aaagaacgct tcgaaggcgg ttgtttttat aggtcttacg gacgattacg
     1381 agtcagaggg ctttgacaga agtactatga agctccccga cggacacaac agactggttg
     1441 aagcggtcag ccgtgtgaac gacaacgtta ttgttgttct tgagggcgga tcacctgttg
     1501 aaatgccttg ggcagacgac gttaaggcca ttctcaacgc atacctcggc ggacagtcgg
     1561 ttgctccggc aattgttgat gtgctcacgg gcaaggcaaa tccttgcggt aagcttgccg
     1621 aaacatatcc cgtttgtctt aaggatactc cgacatcgtt cagatatccc gattcaaaag
     1681 aggatgttca ataccgtgaa agcattttca tcggctacag atattacgac aaggttgaaa
     1741 gaaatgttcg attcccgttc ggcttcggcc tttcatatac gagctttgaa tattcggata
     1801 ttaagctcaa aaagaaaaat cttactaaag gcgagggagc aaaggttact ttcacaataa
     1861 agaatacggg cgatgttgca ggcagcgaga ttgcacaggt ctatgttgcg aagcccgaaa
     1921 gcaaaatatt cagagcaccg aaggagctga aaggcttcgt aaagatacac cttgatcccg
     1981 gcgaggagaa aaaggttacg gttgagcttg acgacagggc atttgctttc tggaatacgg
     2041 cgacagaaga ttggtgcgtt gaaagcggag aatataaaat tctcgtcggc gcatcgtcga
     2101 gggatataag gcttgaagcc gctgccaaga tgaagtcgga ggacgatgag acaattgttg
     2161 atttgagaga gtcggcaggc gtttacttcg acggcgaccc ggcaagagca agagaggacg
     2221 actttaaggt aatctacggc ggtgaattta agcttgctcc ggaaattacg agtgacagct
     2281 taaataactc aatcgagaga agtaaggata agggtcttgc aaagtttatt tataacacgg
     2341 ttgaccttgc catgaagccc aagggcggag tcggcagctc aatgattaca aatacaatca
     2401 tgcaaactcc gataagaaac tatgtttcaa tgagcaacgg actgttcacc gaagatatgg
     2461 cggacggact tctcaagggg tttgagggga aggacgttgc aaagggtgtc ggcaaaatcg
     2521 ccaagggcgt accgaacgca cttgttaatc ttaagtcttt gttgaaatcc atataaaagc
     2581 atttcgcaca ttttaattgt gcggtgctac caaaaaaatg aagggggatt atttggaaaa
     2641 tgaataagac aaagaaagta agcagaaaaa ttatcgcttt tgtgctgacg ttgataatga
     2701 ttttatcaat cgttccgatg agctcatcgg cggcagatgc gataaagaaa ggccttaccg
     2761 cggacaagga agttaacttt gccgttatgt cggatatgca ctattatccg gtgacactga
     2821 caggtaatta caacgaggct ttcatgaaga gcaccaagac tgctcttgca agggagccgt
     2881 atcagtcggt cggtattctt gaatcggctc tcgcaggaat tgcggagcat gccaagaaga
     2941 acggcatgaa gtatcttatc ctttcgggtg atctcacctc caacggtgag tatgaagccc
     3001 acagacagct tgccgcaagg cttgaaaagt tcgagaagga tacgggtatt caggttattg
     3061 ctatcaacgg taaccatgat atcaacaagg caaacggaac tacatacgaa aacggcaagg
     3121 ctgaaccgac aaagcgcaca acgcccgagg atttccttga aatatacaaa aatctcggct
     3181 atgaccttgc ttatcacaga tacactccgt caaagggcaa ggcaaatatg ctttcatatt
     3241 ccgtccgtgc cgacggctac agattcatcg ttatggacac gggcaaatac agctccgacg
     3301 taacggaaaa gggcaaggac cttgccgaaa cggcaggctg ccttacaacc gaggctacca
     3361 actgggttct cggtgaaatt gccgatgcaa aggcaaaggg cgagacggtt ataggcgtca
     3421 gtcaccataa cttcgttccg catttcacag gcgaatacac aatcatcaga ggcttcgtta
     3481 tcgacggctg ggaggagctt acggacaagc tcgttgatgc aggtatgcac ttctcgttta
     3541 cgggtcatat tcatgacagc gacatcgctc agactgttac cgatgacggc gaaacactga
     3601 cggaaatctg tacggattca ctgactgctt tcccgaacta tttccgtgaa gtaagcgcaa
     3661 ccaccgatgt taacggcaag acaacaatga aggtcgagag taaggacgtt gactgcgttc
     3721 ttcccgttac tgttaacggc gagacatacg caacgcctta cagaatcaag tcgctcggcg
     3781 attcgttctt cggcgagggc ggactttctg caacagctct caatatgctt gagggcttgc
     3841 tcggcgaata ttccgagaag tttgccaagg acggtatggt tgcgacgctt aagggtatgg
     3901 gtcttgatat tgaaggaaag ataagcggct tcttcggcga cggtctcaaa atcggggata
     3961 cagaagtttt cacaaccaaa aatcttatgg gctttattga ggatcttctc aatcagattt
     4021 atgaaaatta tctgaccgat cccgacgcaa cggctaagta tcttgttaac tcaatcaata
     4081 agcttctcaa tgttcaggtt tcggagcttc cgaacacgag attcattgat gaatacggct
     4141 ttggcgacag atccaagcct ggcacattcg aggatcttct tgaatgtata attgtttata
     4201 aatatgaggg taagctccat atgaaggacg atccgttcat gatggacgct atagatcagc
     4261 tcaataacgg cgataccgta tttgatattt tcgacgcact cattgacatt gtttcaaatg
     4321 atcttcttca ggataagctt ctcaaggatc ttgatcttaa cctcggtgca ttcttcccca
     4381 agggaacgac gcttgaatgt gtcggcaaga ttcttgctgt aacaatgatg gtcattttcc
     4441 tcggtgacac aagctacctc aacgtttcaa ataagattct tgaggctgca aataagctcg
     4501 gtgtagttga cttcaagtcg ctttggggca ttgccgaata ttacatgggc gaatatctta
     4561 ccgatactca gctcgagggt gtcggacaga cgctcgcaaa cattgcgtgc gaatttgcat
     4621 atgacgataa ttatatcgag gatgttaaca caaccgttat ttacgacggc aaggttacac
     4681 cggttgctac aagagaaaac tacagacttc cgacgatcgt ttcaactacc ctcggcgcag
     4741 atcagagctc aagaaacgta agctggtaca caaagacgag cgtcaagggc acggatattg
     4801 aaatcattcc ttattcggag aaccctgtat ttacaggcaa atcaatggtt ccctacggcg
     4861 taaaggttaa tgttaaaacg gagagaaccg agagacagtt cccgggagtt gatctcggcg
     4921 ttatcggaat catgaactat aagttcccga tgaacagaca cattgccgag gtttcgggac
     4981 ttgaagcagg caagaaatat ctttaccgtg tcggtgatgc ttcaagaaac tggtggagcg
     5041 atataggcac attcaagatg gctgacggtt cggatgaaac ttcatttatt catatctgtg
     5101 atccgcagtc gcagtcggag cagcagtatg agacgttctc taaggtaatt gaaacagcct
     5161 ataagatgta cgacagcgac ttcatcatca acacgggtga taacgttgac cacggtgata
     5221 acttcagaca gtggcagtgg cttttcaaca cggcatccga aacacttatg aacacaacga
     5281 tgatgtcggc ttcgggcaac catgagggca tgggctctta cgcaattgag aagaactatt
     5341 attattcaaa tgttcccgag caagacacag aatcgggcat ctacttctcg tttgactaca
     5401 acaacattca tgttgctatt cttaatacgg aaaatctcaa tgacgataag agtctcagcg
     5461 acgatcaggt tgactggctc attgacgata tgcagtcgag cgatgcagac tggaagtttg
     5521 ttgcgttcca caaggctatc tattcaaacg gctcccacta caaggacaag gacgtttgca
     5581 agataagaga cgagctttgc aagcttatgc cgcagctcgg tatagatatg gtattccagg
     5641 gacatgacca tgtttacctc agaacggacg ccatgatcga caacaaggtt gagagcgtaa
     5701 caacatcaac cgccgagttc aacggtaagg agtacaccgt taaggtgaat cctgtaggta
     5761 cggtttatga aatcagcgga tgctcgggcg ttaagattta caatcagaag gacgagagct
     5821 tgaccgataa gtatttcccg agagccgaag ttatcaaaaa cgttacggct tcaatgttct
     5881 cgggtataag cattgtcggt gatacactct attttgacgc ttacacagtt gacacggaga
     5941 acggcaaaac agaaaatatt gattctttcg caatcaaaaa agacctttcg gttaagaagg
     6001 gtacgggagt taacccgtcg attgactgga tgaagatttt ctcgcaggta attgcagttg
     6061 ttcttcctct cgttaaatcc atcatcagtt gggtgttcga tttcataagc tcaaagatgt
     6121 ggtaaaaaca gcataaaaat atccgggcga tccgattggg tcgtccggtt gattttttat
     6181 aataatattt ctttgtt
//
LOCUS       DAWBSW010000118         6121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2453_length_6121_cov_1.790801, whole genome shotgun sequence.
ACCESSION   DAWBSW010000118 DAWBSW010000000
VERSION     DAWBSW010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_6121_cov_1.790801"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    619..628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1315..1324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2310..2428
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4532..4541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5304..5313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1501 a         1476 c         1667 g         1318 t
ORIGIN      
        1 ctgccgtgag gcaggtgcaa tttgagtggt accgcgggta tgctgtatgc agcccgtctc
       61 aaagaatcat ttctttgaga cgggcttttt tgcatccacg gaggtaacac catgaaaaaa
      121 acactcacag ggctggactt caaaattcag gaaatggcgg gacgcatccg agagctgcgc
      181 gagatctccg gcttttcggc gcagacgatg gcgctgaaaa caggcacgag cgaagaagaa
      241 tatcttcgct gtgaggccgg gcagagcgac ctcaacttcg cttttctcta ccgctgcgcg
      301 ctggcttttg gcgttgacgt tacagatctc attgagggca gcagcccaaa gcttcgatcc
      361 tatacgctga cacgcgcggg taatggacag aagatcgagc aggcccatga gatgatctat
      421 tacaacatgg cctccggctt tcagaaccgc attgcagacc cgctgttcgt tgaaaacaaa
      481 tactctgatg aagcattcta ttcggacatt gagctgacca gccacgtcgg gcaggaatgt
      541 gatcttgtca ttcgcggcag catgaaggtg caggttggcg agcatcggga ggtgctcgag
      601 gagggcgaca gcatttatnn nnnnnnnncg gcggacatac cgagcttctg catgaaggcg
      661 actgcatcta ctatgacagc tccattccgc acggcatggt tgctgcggac ggtcaggatt
      721 gcctgttcta tgcgatcgtg ctccgcccac aggagccgag cctcccggag gccggcggcg
      781 accgcgctgc catgatcgag acggcgcaga gtccggatcc ggaggcgcgc atttacaccc
      841 gcttcgtcga tgcgcccgag gatgcaaacg ggaaactgca gtccatcgcc ttcaaaaatg
      901 agcaatcctt caatttcgca tttgacattg tggatgcgct gggccgtgaa aagcctgaaa
      961 agctcgccat gctgcatgtg gctctggatg gaacggagcg gcggttcaca ttcaaggaca
     1021 tgaaagatgc ttccagccag gccgccaatt actttacctc gctcggcatc cgacgcggcg
     1081 accgcgtgat gctcgtgctc aagcggcatt accagttctg gtttgccatt ctgggtctgc
     1141 acaagatcgg cgccattgcg attccggcta ccaatcagct tgtggagcat gatttctctt
     1201 accgcttcca ggcagcgggc gtcagtgcag ttctctgcac ggcagacggc gatactgcgt
     1261 accatgtcga cattgcagag gccgacgccg ggatgaagtt gacaaagatc atggnnnnnn
     1321 nnnnccaatg ctcatgttct ttacctccgg tacgtccgga tacccgaaaa ttgcagcaca
     1381 caactataaa tatccgcttg gacattttat caccgcaaaa tactggcatc aggtcaaccc
     1441 ggacgggctg cacctgacca tctccgacac cggctgggcc aagagcctct ggggaaagct
     1501 ctacggtcag tggctgtgtg aggcggctgt gtttgtttat gatttcgacc gctttgatgc
     1561 agaaaaaatt ctgccgatgt ttgcgaaata tcagatcacg acattctgtg cgccgcccac
     1621 gatgtaccgc atgctgatca agcaggacct gtcccgattt gatctttctt ctattcagca
     1681 tgcgtctatt gccggtgaag cgctcaatcc tgaggtcttc cgccaatttg aaaaggcgac
     1741 cggcatgcgc atcatggagg gctttgggca gtctgaatcg acgctcatca tcggcaatct
     1801 ggctggcgac agccataaga tcggctctat gggcaagcct gtcccactct acgatgtgca
     1861 tctgctggac agcagcgggc atgaagcgga ggctggcgac acgggggaga tctgcatcaa
     1921 cattcaaaac ggcattccct gcggacttgc ctacgaatac tacaacagcc cggaggtcac
     1981 ggcaaaaacg tggcacgatg gctggtacca tacgggtgac cttgcatggc gagatgagga
     2041 cggcttctac tggtatgtcg gccgcgcgga cgacgtcatc aagtcctccg gctaccgcat
     2101 cgggccgttc gagatcgaaa acgtcatcat ggagctgccg tacgtgctcg aatgcggcgt
     2161 ttccgccgtg cccgacgaga cccgcggcca ggtggtcaag gccagcatcg tgctggtcaa
     2221 gggcacggaa ggcacggacg cgctgaaaaa agaaattcag acctatgtca agacgcatac
     2281 tgcgccgtac aaatatcccc gtattgtggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ccaaagacca ccagcggaaa aatcatcaga
     2461 agcaagctct gaaggaggag tcggccatga cacacaaacg cctaactctt tttgccggac
     2521 attacggcag tggaaagacg aatatcgcgc tgaattatgc gcggtggctc cggcgaaaag
     2581 gcgaagccgt gaccgtggcc gatcttgaca ttgtaaatcc ctacttccgg acgaaggatt
     2641 cagaagaaga tctgaaaaaa gaggggatcg gactgatcgt ctcaccgttt gcaaattcga
     2701 atctcgatgt tccggccatg ccgaaggaga cctacgcgct gattgcggag cggggagaat
     2761 gcggcgtgct ggacatcggc ggagacgacc gcggagcgct ggcactgggg cgctatgttc
     2821 cggagatccg ggcggagggg gattatgaga tgctgttcgt catcaaccgg gcccggccgc
     2881 tgacaaggac ggtagcggat acgatccttg tgctgcggga aatcgaggcc gcctgtgggc
     2941 tgccgttcac ggcagtcgtc aacaatacga atctcgggca ggcgacggcg gcggtggatg
     3001 tgctgtcaag catgccctat gcggaagcgg tcgccgcgcg ggcagggctg ccgctcaaga
     3061 tgacctgctg cgctgcacgg ctctgcgacg cgctgcgcgg gagcattcca aacctgttcc
     3121 cgatggagat ccagaagcta tattaccaat tacgataaat ggaggaacaa gatggcaaaa
     3181 ctgatatttc aggaagaact ctgcaagggc tgcggcctgt gtgtgaacgc ctgtccgaag
     3241 gggctgctgg cgctgtcgaa gacgcggctg aatcaaaagg gacatgcgcc cgtggagctg
     3301 acggagccgg acaagtgcgt cggctgtgcg ttctgcgcaa cgatgtgtcc cgattgtgtt
     3361 attacggtag agaggtgaaa aaaatgtcag ataaagtact tatgaaaggt aacgaagcaa
     3421 ttgccgaggc tgctatcagg gcaggctgcc gccattactt cggttatcct atcactccgc
     3481 agacagaggt tgctgcctat atgtcaaagc gtatgccgaa gatcggcgga accttccttc
     3541 aggcggagag cgaaattgcc gctatcaata tggttatcgg cgtttcgtca acaggcaaga
     3601 gagtcatgac ctcctcttcc agcccgggag tttcgcttaa aagtgagggt atatcatacc
     3661 tcgcaggctg tgaccttccc gcgctcgttg tcaacgttca gcgtggcggt ccgggactcg
     3721 gcggaattca gccctcacag tctgattact ggcagtcaac aaagggcggc ggacacggcg
     3781 attaccacaa tatcgtactt gctccggctt ctgttcagga aatggcaagc cttacggtca
     3841 agggatttga gcttgcagac aaatacagaa tggttgcaat gatccttgcc gacggtacaa
     3901 tgggtcagat gatggaacct gtttctctcg atgttgccga ggttactccg gttgaaaagc
     3961 cgtgggcact cacaggcaca aagtgcgaga gagagcataa catagtcaat tcgctttacc
     4021 tcaaacccga ggagcttgaa ataaagaact ttgagagata tcagaagtac aagacgattg
     4081 aagaaaacga aacaatgtat gaggaatatc tcatggagga cgccgagatt tgcgtagttg
     4141 cgttcggcat tactgcccga gttgcaaaga atgcagtcaa cgaagctcgc aagagaggcg
     4201 tcaaggtcgg catgataaga ccgatcacac tctggccgtt cccgacaaag ccgctcagtg
     4261 ccgccgccga gaagtgcgag agatttataa ctgttgaaat gaacatgggt cagatgatcg
     4321 acgatgtaaa gcttgcaatc gactgcaaga gaccggttga cctttgcaac cgcgtcggcg
     4381 gtatgattcc gactcccgag gatgttcttt cggctatcat gtcaaacgga ggtgcagaat
     4441 aatgtcagtt gtatttgaaa aaccgaaatc attgacagac gcaacgcttc actattgccc
     4501 gggctgtact cacggtatta ttcaccgtct tnnnnnnnnn nagcttggaa tcgagggaga
     4561 tactatcggt gtcgcatcgg tcggctgctc tgttagtgca tataactatt tcacatgtga
     4621 ctttgttcag gcggctcacg gccgtgctcc cgctgttgca acaggcgtaa aaagaagcga
     4681 cccgtcaaag gttgtattta catatcaggg cgacggcgat cttgccgcta tcggtactgc
     4741 cgagacggtt catgccgccg caagacgtga gaatatcacc gtaattttcg tcaacaaggc
     4801 catctacggt atgaccggcg gtcaaatggc tccgacaact cttcccggcc aggtcactca
     4861 gacctctcct tacggcagag acgtaaacac ggtcggctat cccgttaagg tttgcgagct
     4921 tctttcaaac gttgacggtg cagcatacct tgagagagtt gccgttaaca atgttaagaa
     4981 cgtcaagaac gcaaagaagg caatcaaaaa agctttcaaa aatcagattg aaggcaaggg
     5041 cttctcactt gttgaggtta tttcaacctg cccgacaaac tggggcatga ctccgcagaa
     5101 ggctcttgac tgggcagagg agaacatgat tccttactat cctctcggcg tctacaagga
     5161 tatctatgca aaggaggaag aaaaatgagc aacacttgca gaatgttatt tgcgggcttc
     5221 ggcggacagg gtattctgtt cgcaggcaaa ttccttgcat ataaaggact tatcgagggc
     5281 aagcaggtaa gttggcttcc ttcnnnnnnn nnngtcccga aatgagaggc ggtactgcta
     5341 actgttcggt aatcatcagc gacgagccga taggctctcc gatcgttgac aagccggacg
     5401 tacttgttgc aatgaatctt ccgtcacttg aaaaatatga aaacgaagtg gtcagcggtg
     5461 gaaagatttt tgttgattct acaatgattg acaaaaaggt tgacagaacg gatgtggatg
     5521 tatattatat acctgcaacg aaaatggcga acgataacgg aacgccgacg cttgccaata
     5581 tgattatcac gggcaagatt ctcaaagaag tgccgcagtt ttcaatagac tataccgatg
     5641 acgcactcaa aaaggttgtt tccgcccgtc accttgatct ccttgatgta aacaagggtg
     5701 ctgtcaaggc aggctatgac tatgaataat aagatgagaa tttcctgtat tcagcttgat
     5761 atgaagctcg gcgatgttga gtataactat gccgaggctc tcaggctgat aagagagact
     5821 gttgaaaaag aaaaatccga cgttgttgtt cttcccgaaa catggacaac gggtttctat
     5881 cccaaggaaa accttgcgga ttattgtgac agagacggcg agagggtaaa aaagactttc
     5941 tcggctttgg caaaggagct taacgtaaac atcgttgcgg gcagtattgc caatgagaag
     6001 aacggcggaa tttacaacac ggcttttatt ttcaaccgtg agggtgaagt cgttgccgaa
     6061 tatgacaaga cgcacctttt tactccgatg gacgagcaca ggtttttcag cttcggcaaa
     6121 a
//
LOCUS       DAWBSW010000119         6068 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2472_length_6068_cov_2.040412, whole genome shotgun sequence.
ACCESSION   DAWBSW010000119 DAWBSW010000000
VERSION     DAWBSW010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_6068_cov_2.040412"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    911..920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1555..1564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1846 a         1178 c         1357 g         1667 t
ORIGIN      
        1 atacaattcc cgccagcaag cttgtcagca tggaaaacga gataagcaat gtcaagggcg
       61 tcagcagtat gcttgcatat gatatgtttg tcggaacttc aattccagat tcgattgttc
      121 ccgatgatat tattgatatc gtcaaacaga acggacggca ggtaatgctc gtcaactcta
      181 tctatgaatc ctcgacggac gaatgtaaca gtcaggttga agagattgat aatatagtcc
      241 aaaaatatgc aggttcagac cattacggct atgttacggg cgagggagca ctctataagg
      301 atcttatcga aacgacaaag gttgatttcg ccgtaacgag cattatatcc ataattgccg
      361 tctttattgt tatagcgata gtttttaaat caatttccat accttttatt ctaattcttg
      421 caattgaggt tgcaatatgg ataaatcagg gcatatcaac agttttcgga acagcgattc
      481 cgttcattgc tccgacggta atttcgtgta ttcagctcgg cgcaacggtt gactacgcta
      541 ttctgctgac atcgcgattc aaagaggagc tttcaaaggg tcacggaaga accgccgcca
      601 tgaagattgc ggcaaatgag gcgatacgtt ctgttttcca gagtgcaatg cttttcttct
      661 tcgcaacctt cggagtatat cttgtttgca gcatttcaat tgtcaagtcg atatgtgcaa
      721 tgcttgcaag aggctcttta ataagtgctc ttgtgattgt attatttgtt atgcctattc
      781 ttctcctttg cgaaaaattc attgcaagga caacaaaggg ctggggtatt cacgatgaca
      841 ctcccgagcc ggaaccggaa aaggccgttc cggaaaagaa gaatgttgct gaatacgaat
      901 ttgatgaaga nnnnnnnnnn ttctcaggac gacagcgagg aagaagctaa ttccgttaga
      961 tacaccgtcc ctgccgcatc ggttaaaaaa accgaaaccg ttgatgtcaa tcttgacaat
     1021 acccgcactg agaaaagtat aaaggtcagc gattggcttc atgttccgca aggcggcgta
     1081 tatgttgacg acgtaacaaa tctaacatct gttgtcaacg ttaaggacga ttcgaaaccg
     1141 caggttaacg gatcaacgct tcgctggcat atgagcacga ccgatcttta ttaccagggc
     1201 gactacagcg gcaagcttcc gcttgaattt aagatcaact atacccttga cggtcaaccg
     1261 atttcctcca aggatatcgc cggcaagagc ggcaaagtga aaatcacaat caaaatgaac
     1321 aacgttgata aatataccgt aaaggtcaac ggagttaaca tgacaatgta taacccgatg
     1381 cttgttgtcg gaggaatttc actcagtgaa atgaaatttc agaatatcga agttgaaaac
     1441 ggacgtgttg tcggcaacgg taacacacag tatgccgtgc tcgccggatt ccccggtatc
     1501 aatgaaagtc tcggactttc ctcgcttgca tccgattcgg attcgacata taagnnnnnn
     1561 nnnngttcaa cgatactttt gttatttccg ccgatgtaaa catttttgag attggcaact
     1621 tcatgttttc ggcaattccg atcgcatcgc ttgatatcgg tctaaacaat atttccgatt
     1681 ccgttgacag ccttcgagat aatcttcaaa agcttcagaa tgttcaaaaa agccttcagc
     1741 agatcgacgc atcaaagctt ttaaatacac tttcttcaaa tcccgacaag ctcaataatt
     1801 taagcgcact tgttaagcag gcttcatcgc tctacagcga gaacgccgct ttgataaatg
     1861 ttcttaataa atatacaacg ccgcagaatc ttgcgacaat tcaggtgctt gttgaatata
     1921 taagaaccgc cgatttcaac ggtcttgagg atgcactcaa ggtaatcaac agcattttcg
     1981 gtgacgaagc gagtcaggaa acgataaatc aaggcatgca gcttcttaag gagatgtcct
     2041 ccgacctcaa tgatcctcag gttcaaaaag cgatagagaa tcttccgaaa accgtaaata
     2101 cgctttccga gcttcaaaag gcggttgagg agaacaagga tcttataaat gcgcttcagg
     2161 cgctttcgga gtcgaacgca ctttcttcga ttgattcgac gctttcaagt cttaaaggct
     2221 caatcgcgac cgacagtctt tcttcgattt cgggaattga cgcaaacact ctttcggcaa
     2281 aaatgacggc atggcttgag ctcggtaagt catacactat ttttaccaag aaagctcaga
     2341 caatgtcatc aagcgttatg tttgttttca aaaccgacag cgtgagagtt aattcttcaa
     2401 acaatgaaaa agaaagcacg accgcagccg aggaaagcac agcagcttcc gatgagggcg
     2461 gcattaaagg attttttaat aagatattca aaaagagcga gggctgataa tgaattgcaa
     2521 ggtttcagtc attattcctg tttataactg tataaaatac ctcgaaaacg ctgttaaatc
     2581 cgttatctcg cagaccgaat ttgaaattat tgagttgatt cttgttgacg acggttcaac
     2641 cgacggctcc gagaagctat gcgaccgtta tgcggaaatg tatgataata tctcggtaat
     2701 tcatcagaaa aactcgggag tttccgttgc tcgaaacaac ggaatcaacg tagcaaaagg
     2761 ggagtatata gcttttcttg acagcgacga tgaatataag ccgagcttta ttttggaaat
     2821 gctcaaaagc gccgatgcag accttgtctg ctgcgattat ttcatttctt ccgtagatga
     2881 aagaaatgtc ggactttatt ttaaagccgg aaaatattca aaatatgaat ttgaccttga
     2941 tttttttaaa tgtaccgtgc attcttgttt ttattcctgc tggaacaagc tttataaaaa
     3001 agatattatc aaaaaaaatc acgtctcatt tccggcaggt gtgaaatatg ccgaggatat
     3061 ggtttttgtt tttgaatatt tgaagtattg tgaatctttt gaatttattg acgaaccgct
     3121 ttaccgttac aatgtcaatc ccgacaatgt cacatatgtt gtaaaaaacg gattcgatgt
     3181 tcagcgcttt atttatgaat atcaaacaag atatttcgaa gatgcttttt tcaaggatga
     3241 tatattgaat gagattaccg agaattttgt ttattttaca actaactccg ttaactcgga
     3301 gatcacttac ggaagtattc cggcaggata taaatatgtt aagcgtgttc ttgcaagcga
     3361 tttttacgat ttgtatttga aggcggatta ttctgaattc aagtgctttt atgacaaagt
     3421 atttttcacg ctgttaaaaa aacgtatggc gcttgccatt gtactttggc gaaaactttt
     3481 tgatttaagg agcaagcttt tacatgactg atttaataaa actaccgtca tttgaatggt
     3541 ttttaggctt gatgggcttc gggggaaaag gcaataccta tgccggatca tacggaacag
     3601 atccttacct cggctgtctc ggaaggccgt cattcagata cagggcatgg atcgaaaagg
     3661 acgggaatga tgaaaagcaa ttcaaagccg tttattatat cggcaatgac tgctacgatg
     3721 aaaccgataa agaggttatg acagagaaaa catttgaggc atcagccgca ggaattcttg
     3781 aagcgcagaa atggctgctt aaagagcttg atgcttttaa cggaacaaca aaggaggcgc
     3841 agcaatgagc aatttgaaaa cacgctggac ggataatgtg aattatgact gtcctctcag
     3901 tgaatatcca aggcctcagc ttgtcagaaa cgactggcaa tgccttaacg gaaaatttga
     3961 gtttacaatt acaggcatga ctgacgatat tcctcaggat tttgacgggg agatagttgt
     4021 tccttttgca ccggagtgtt atctttcggg aattgaaaga acaatcgaac ctgatgatta
     4081 tctttggtac cgaaaaaaat ttatacttga cgattgcttc gacggaaaac gctgtattct
     4141 tcattttgag gctgttgact ggcgctgtat ggtatttttc aacggtaaac ccgtcggaac
     4201 acatcagggc ggttatcttc cgttcagctt tgatgtaacc gaatatctca atgagggcta
     4261 taatgagctt gttgttcgtg taaatgaccc tacggagacc aattggcagg ataggggaaa
     4321 gcaggtcaga aaaagcgtcg gcttctggta caccgccacg agcggaatct ggcagagcgt
     4381 ctggcttgaa ccggtaaacg aaaagcacat tgaaaatatc cgttttactc ccgacattga
     4441 cgcttcggcg ataagaatcg agactgaact gaaatacgcc gagaatacac agcttagggc
     4501 gattatcaaa aaggatgatt caattgtttt tgccgggacg atttctcccg acgaaacggt
     4561 taaactcaag gacattcgtc tttggagccc cgaaaaccct tttctatatg atattgtaat
     4621 tgcactttgt gacgaaaacg aaaaaatact tgatgttgtt tcgagctatt tcggcatgag
     4681 aaaattcagc ataggctatg acgataaggc gattccgaga ctttatctca ataacaaacc
     4741 gtattttcag accggacttc tcgaccaggg ctactggccc gacggaggta tgactccggc
     4801 ttgcgatgag gcaatgattt ttgacatcaa agagatgaaa aatctcggct ttaatatgct
     4861 cagaaaacat attaaaattg aaccgagacg ctggtattat cactgcgaca agctcggtat
     4921 gatcgtttgg caggacatgg tatgcggcgc aaaaaagatt gatatgtttt ttgtcggcgt
     4981 tctgccgaat atgtttatca gaagcattcc cgacagccat tataaatggt tcggcgttga
     5041 taatgaggaa tgtcgcagag agcatgaaaa ggcaatcttt gataccgtag atcatcttta
     5101 taactgcaca tcaataggct gctgggttcc tttcaatgag tcgtggggac agtttgatgc
     5161 aaaaaaaatc ggcgaagctg ttaaagcgta tgaccccaca agaatagttg accatgcgag
     5221 cggatggcat gatcagaagg gaccggagct caagagcgtg catcagtata ttcttccgtt
     5281 cattccgcct aagggagacg gcaggccgct tgttctcagc gacttcggcg gctacagccg
     5341 tataatcgaa aatcatgttt ggaacaggga gaaaagtttt ggctatgtca tgtataagac
     5401 aaaggaaact cttacagggg catataaaaa gctgtttgaa aagcagatca ttccaaacgt
     5461 taaaaaaggc ttgagtgcaa cggtttatac acaagtaagc gatgttgaat ttgaggtcaa
     5521 cggaatctat acatatgata gagagcttct taaaatcgac gctgacacga taagagaaat
     5581 caacgataaa ctaaagacaa tgccgaataa ttgaggtgat cggatgaata ctggcgacca
     5641 taatcttaga cttttgttcc tttcggatat ttttaacaga caaacggacg aggagcacgt
     5701 atattcagcg gctcagctta tttcaatgct gggcgaatac ggcattagct gtgagagaaa
     5761 atccatttac cgtgacattg aggatcttaa ggaatacggt atggatatcg taaatatcag
     5821 aactcctcac agagggtatt atcttaaatc aagagatttc tctgagccgg aggcaagact
     5881 tttaattgac gcggttcaag ctgcgaagtt tatttccgag aagaacacaa aaagtctgat
     5941 ttataaaatc ggcagattag tcagcgaata tcaggaggag gagctcagag aacaaatata
     6001 tgttgacggg agcttcaaaa cagataaaga agatctttat gaaacgatcc gtgttctcga
     6061 taaggcga
//
LOCUS       DAWBSW010000120         6066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2476_length_6066_cov_2.336051, whole genome shotgun sequence.
ACCESSION   DAWBSW010000120 DAWBSW010000000
VERSION     DAWBSW010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2476_length_6066_cov_2.336051"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1515..1524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4652..4661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5606..5615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1643 a         1364 c         1297 g         1732 t
ORIGIN      
        1 gaactgtggt ttttcccggt aaaaatgctt tcataaaaga actgcttgaa attcaccccg
       61 agataacaac tatcgttcaa aatatcaaca attccgatac gagcatggtt ctcggtgaaa
      121 cagaaaaagt tctttacggc aagggtcata ttaaggactc gctttgcggc tgtgttttcg
      181 gaatttcgcc gaaatcgttc tatcaaataa atccgattca gaccgaaaag ctctacaata
      241 aagcgatgga gtttgcgaat ctcacgggta aagagaccgt aatcgacgct tattgcggca
      301 tcggcacaat aggtatcgtc gccgccaaga aagctaagca ggttatcggc gttgaggtca
      361 accgtgatgc cgttcacgat gcgattgcca atgccaagct taatgataca aagaatattc
      421 gattttactg cgccgacgca ggcgaattca tggttgacat ggcttcgaac ggcgaaaaag
      481 ctgacattgt tttcatggat cctccgagag cgggaagcga caaggcattt ctttcatctg
      541 tcgtcactct cagcccgaag aaaatcgtct atatctcctg caaccccgaa acacaagcaa
      601 gagacctctc tttcctcgtc cgcaacggct ataaggtcag aaaaatccag cctgtcgata
      661 tgttccccca cactgcacat gttgaatgta ttgcactttt gacgagggta aaataatatg
      721 gctgtttacg ttaatacaaa aaacatgtcg aagaaaacgc aggcatggat atgtattatt
      781 ttcggtctga ttgtcggaac agtgtttacg ttcggtatgc aatattggaa ctcgccgatt
      841 acaaaagacg aagcaagata ctccgaggct attttttcct tttataaaga aataagcaaa
      901 gacggcaatg ttaatgagat gattttatat tttgaaaatc acgatgaatt aaccatcgat
      961 aacacatgtt gcagtcagga agtagttgat aaaattcgtt ctctcaaaga gggtgcaatt
     1021 gtgaaaatat actaccatcc caacagcaac aaaatcatgg aaatgacttg caacagcaac
     1081 ataattcttc aatttgatga ctctgcctca aagctttcac gggatagaca aggctttaca
     1141 gtactcggaa ttttcatgta cttcgtgacg gcttacggcg tcacaatgct tatacgggaa
     1201 aggaaacatt caagcgaaaa atataccacg gaggttgatt tatgacctta aaagaagcta
     1261 taaaatacag aaaaatatgg atgggctttg ctattttgtg gatagtcttt tttcacttcg
     1321 gctacaaact gcccgatcct ttttccgtca tacagtcgag cggctacagc ggcgttgaca
     1381 ttttcttttt tgcttcgggt atcggctgtt atctttcata ttctcgtgac aaaaatgccg
     1441 ccgcttttct caagcgacgc tttataaaaa tcatgcctac atatctggtt ttcatcgtta
     1501 tatggtgtct gtatnnnnnn nnnngtataa ggtcataacc gcaggtatgg attttccgac
     1561 ggtgctcggc aacattttct gcattcagga tctcaccggc aagcccggag gatttaactg
     1621 gtatttcggc gcaatgtgga ttttttatat tttgacgccg tactttgccg ccgttgtcga
     1681 taaaatcaaa aagcctgtcg gatgtgtcct tttctgtctg ctgctggtgc tgttctcctt
     1741 cccgttttgg aacgttaccg cccttatatt aattgtaaca aggcttcccg tttatttctt
     1801 gggaatgttt ttcgcaaaat gctcgcagga aagcgataaa aaaatcacgg caaaatttat
     1861 gctgtttgcc acacttcttt cgctattgag catggcggca tttttcatat gcttctttaa
     1921 tctgcatgat gagctttgga actacgggct gtggtggtat ccgtttataa tcaccgcacc
     1981 gacaatctgt atttaccttt catttatcgg aagatttctt gaaaagaagc cgttcgggca
     2041 aaaaataaat gcagcatttt cgtttgtcgg cggctacact ttcgagcttt ttctcgtgca
     2101 caaattcatt ttcaatgaga cggctgactt gatcatggca aacaagcccg atcacgttgt
     2161 tgcgatttgg tttgcctccc ttattcccgt cgttatcggc actgtgattc tttattggct
     2221 gacaaaaggc attaaagctc tcatattcaa atcatcaaaa taacaaaacg gcagtaacat
     2281 tgaagctact gccgtttttc aatcttaata aatctgtaat tttgacagga agtcctgcat
     2341 gccgtccttc attttgtcgc tcactgcgtc gaaatcaacc gttgcaagca acgagcgaac
     2401 cgaaccgata tccgtattct ctccctctgc tacggtaaag ttgcacttct tcccctcaag
     2461 cttgagcgtg tactcacctg ccggaagctt tgcgctgaaa ttgcccgaag tgtcggtcat
     2521 cattctgtaa accgtgccgt cacgattgct cgttacagta aaatcaatca cgttgctcgg
     2581 cgtgattttc gcccatttaa gatgtccgtt taccgtgccg tagttttcgc actcttttat
     2641 tgccgcttca actgcaagct gagcgtttac cattcgcatt gtgcccgtcg gcagatgaag
     2701 cctggacgag ctgtcggcga cctcatactt tgtgttctcc tcgtcgcaga caaaatgctt
     2761 gacctgagca cccgtaaaag aaggattgac gctccaaacc agtgaagcga tacccgttac
     2821 tatcggggca gccattgatg tgccgttgag gtaactgtaa gagcaattgt tatattggtt
     2881 accatcgccg ttgtaatacg cgctgtaaat ttcataaccc ggggcacaaa ttgaaacctg
     2941 actgccgccg tttgaaaact ctacctgctc aaaccggttg ttgccgttga actttgccgc
     3001 cgcaacaaca attattctgt caacaatttc ctgcggcgta acgcccttga cggactttat
     3061 tgcgttgtca accgttatcg ggcagaatgt gccgttattg cttgcgtcaa cggcataggc
     3121 aaacttatcg tttttgtaaa cgccattgcc tgccgattga cagcagataa aatcatatcc
     3181 gcgttggagc attttggcaa tgtaatatga attgaacttg ccctgagtat ccttaacgat
     3241 ttgcagcctg tcaattccgt tttttattgt gccggaactt ccgagagaaa aattgactac
     3301 ttttgctcct gcacgaacag cgttgacaaa gccggtcatt attcttgcac tgttgttcca
     3361 tttttgcccc tgcttttcat ctgcctgcca atcaacgcaa ataagcttgc cgtcgcgaac
     3421 gattcccgct aaaccttttc cgttatcctg ctgtgctccg ataattccgg caacatgatt
     3481 gccgtgatac gagggagcat tatttttttc aaaaaattca ttcgggaaaa cgatctttcc
     3541 ttcaaggtct tcgtgagtcg ggtcgaatcc cgtatctaca attccaacat cgatatcgct
     3601 gaaatattcg tcgtaatccc aagccttatc gacatctgtc gcctttatcc accagtttga
     3661 gaacgtaaac agtctgtcaa gcgcatcgtc ttcccaatag gtaaagttcc acggatcttt
     3721 cggaacataa tcgggcgtta tcttctccgc taacgaacag cttgcgaatt cgaccttttc
     3781 attttccata agctcttcgc acaggtcatt gatttcataa aaatccgaac gttcaatttt
     3841 tatttcgtat tcgttcataa aatcaacctt accgactatc tcaccgtcaa gtgacttaac
     3901 aacattattt ttatcgagaa gcgttgcatc gtcgctgaaa aacaccgtta cagcatcgtt
     3961 tatgtaggtt gtagagtctt caagagtcga tatactctcc tccttcggtt cataaaaaat
     4021 atcgtcctcg tcttcaaacc gagcgatatt atttgcattg atttcaactg tttttgccga
     4081 attcttttct gccgaagcgc tttttgccac ggtcctttca accgttcttt cggcttttga
     4141 aacggcctga accgtcggtt cgtcagatag acgagcttcc tttgcaagct taatgccgac
     4201 tgtcggcgaa cctgcggcaa taactgccgc caacgcaaaa gaaaggacgg agcttaaaat
     4261 cttccgcttt ttcattttta taacacccct attttacata taattgtatg ttactatgtt
     4321 aagcagtaaa agtcaatgtg attttcaata aaaattgaac tatatttata atttttatag
     4381 aaaatatata gatttttaag ttaatgtatg atattatata tgtatattta ttattcggag
     4441 ggctgaatat gacggtaaaa gaaatcattg atatactcga cggacatata atctacggcg
     4501 aggatctgct tgacagagag gtgtttaccg cctgcggcag tgatatgatg agcgatgtgc
     4561 ttgcttttgt caaggagcag gcagttcttt tgacaggtct tgttaatccg caggttgtga
     4621 gaactgcgga aatgatggac atgagctgca tnnnnnnnnn nttttcgtaa ggggtaaatc
     4681 gcccgactta agcatgattg aacttgccga gcagcgtgac attgttctca tcggaaccga
     4741 gcttgaaatg ttcacctcct gcggccgtct ctacgctcat gggctcagag gaggcagcgg
     4801 aaaataatgg acgagataaa attgcattat gacgtcagtg cctatgactt cacaagagca
     4861 ggtgaagcat cgggcaagct gaagcgttct cttaaagagc ttgggttctc ccctgcagtc
     4921 acacgcaacg ttgcgatttc tgtctatgag gcggaaataa acatggttat tcatgccgac
     4981 ggcggagtta tcgacgcaag cataaccccc gaatgtataa ccgttgtgct ggcagacaag
     5041 ggaccgggaa ttccaaatgt cgagcttgct atgaaggcgg gttattcgac cgcacccgag
     5101 aatgtgcgtt cgcttggctt cggtgcaggc atgggtctgc cgaatattaa aaaatacacc
     5161 gacgaaatga acattgatac ggttatcggt gagggtacaa caatcacttt aaaaatatac
     5221 aataaataat tatctgaaag caaggagtag atcatggata ctgtttttca ttcggttagc
     5281 cttgataagc aaaaatgtct cggatgtatg aactgtataa aaagatgccc cacccaggcg
     5341 ataagagttc gccacggtaa ggcaacgatt atttccgagc gctgtatcga ctgcggagaa
     5401 tgtattcgca tctgtcccca tcacgcaaaa accgctaaaa gcgatccgat agaggatatt
     5461 caaaaattca aatataaaat tgctctgccg gcgccgtcgc tctacggtca gttcaataat
     5521 cttgacgata tcaattacat attgaccgct ttgacggaac tgggctttga caaggttttc
     5581 gaggttgcaa agggagccga aatcannnnn nnnnntctcc gacgcaacga gaaagctgct
     5641 gaaaaacggc aatctgaaaa aaccgattat ttcctcggcg tgtcctgccg tatgccggct
     5701 gataagaatt tcatacccgg atcttatcga caaccttttg gaactaaaca ctccgatgga
     5761 tgagtcggca cgtttggcaa gaattgaagc gagagagcaa acaggccttg acgacagcga
     5821 cataggcgta tttttcatta ccccctgccc tgccaaaatc acggcgataa agaatccgct
     5881 ctgcgccgag aaatctgacg ttgacggagc aatcgcaatc agcgatattt atcccatgct
     5941 tgtcagcaaa atggatcacc tcaaggagct taaaagcata gatgaatcgg gccgaatcgg
     6001 cgtcggctgg gcgacaagcg gcggtgaagc ctcaggctcg ttgagccaga aatatgttgc
     6061 cgccga
//
LOCUS       DAWBSW010000121         6065 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2477_length_6065_cov_2.928286, whole genome shotgun sequence.
ACCESSION   DAWBSW010000121 DAWBSW010000000
VERSION     DAWBSW010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_6065_cov_2.928286"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4660..4669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5059..5068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1577 a         1381 c         1272 g         1815 t
ORIGIN      
        1 ccgttatatg tcggtgtggt gttgtattcc cacttgctct cctgaagaac atcatttccg
       61 ttcttgaagg ttacagtgta ggacttgatt gaccactgag catgaagtgt tatatcttcc
      121 gaaataactg ttgtttcttc aaccttctgt cctgcctcat taaaccaacc gctgaaatcg
      181 taaccctcac gggtcggtgt cgggaactta accgctacat cagtccagat cgcataaagg
      241 ataaccgtat caccgtcagt cgatgaaaga ttggttacgc ttgcaccgtc tgaatattca
      301 gcggcgccgg tttcggtttt tgcccaaccg ttaaagtttg atactgccgt agccgttgtt
      361 gtcgttgcac cgctgtagtt ataatcaaac gttacggtga actctctctt gaagccgttt
      421 gccgtgagag cctttgcaac atcataagta tgtgatgagc ttgcggtttc accgctcgtt
      481 gcgccgtttc cgttataaga aacagtataa gtaatcggag tccagtgagc ggtaagcttt
      541 gtcgcggcgg tcggagtaaa ggtctgactt acctttgttc cgtcgcttgt ataccaaccg
      601 tcaaaagtat aaccggttct ggtcggagtc gggagttcaa cgcttgaatc agcccactta
      661 gcataaagat caataatttt accgtcctct gcggtgaggt tcttaacgct ctgcttatct
      721 tcgtaaactt tattgccgtt ctccgatgtt gcccagccca ggaattctga agttgcagta
      781 gccgtcgttg aggttgaacc gctgtagtta taatcaaacg ttacgtcaaa ttcacgcttg
      841 aagccgtttg ccgtgaggtt ctttgcctca tcgtagatat gaaccgaatt gcctgttgat
      901 cctcctgtgg agccgtttcc gttgtatcta acggtatatg agatcggatc ccagtgagca
      961 taaatatcaa catcacggtc gagggtatcg tcaaagctcg gcgtacctaa acttacagcc
     1021 gtagatccgt ctgtaattgc atcacctttg gctctgactg tgtaccagcc gaggaattta
     1081 tagccctcct ttgtcggagt tccgagacct gaacatgttg aatggagatc aataagtgca
     1141 ccggaggttg ccgttacatt atttacatag tccatgcttg catggaactt gtagttatcg
     1201 aactgaacgg gaacattcgg atttaccgtt gcaaatatct ctaccctttc gcctgtcgaa
     1261 agtgctttaa gcgcatgagt tgaacttgtt ctgctgatag ttgatgtata taccgttgcc
     1321 gaacagccca ttgcgttgcc gccgacatag tatgccgaga aataaacgtt cttcgcttcg
     1381 ggatttgttc ccgaaagaat ttcgatcgtg cccgttgccg tcggagtacc gctgagagtg
     1441 aacaaatcgg acatagtatt atatgttacc gtaacgccat tttccgtctt tgtatatgtc
     1501 ggataatatg tctttgttac tccgtcaagc ttgatatcat agggcaagcc ttgtgctcta
     1561 tagccgctct ttgccgtgtc tgcgataaga tcggcaatga ggttacggcc gacattcttt
     1621 gtgagggtgt taagctgcca tttaagtgcc ggatagtcgt tgttcacgcc ctgcttataa
     1681 tcataggcat tgccgaccga gctgataccg gaaaccgttc tggttgtgtt cagggctgca
     1741 agagccgccg tgcctttgcc cattgtattt gtgaagcatc cgtattgagt aacgccgccc
     1801 gaaccgacaa gctttgaaac cgacgcagta accgagaatg agttatacgc caatgtggtg
     1861 ttgccgttgt tcgtgccgat aaggccgcca gcgtttgtgc tgctgctgat gcttgaagcg
     1921 ttgttataag aatcggcaat aacagcctgc tgattaactt cgccgaaaag gccgccggca
     1981 tttttcgctt tatagcttac cgaagcaata cgtccgtctt ttccgttgcc gttgaaacac
     2041 ttggacatat aaatctttac agtgctttcc cttgcattaa gcttaccgac aataccaccc
     2101 atatccgcaa agttattggt agttgcggaa aggttgccgt agttaccgca cttggtaaag
     2161 ctgaaagttg ttccgttata tgcgttggaa acttcgccga ggataccgcc gatattgttt
     2221 ttagcggtca cattgccata gtttatgcag tctgcaaagg aacttgtatc gtcctcgatc
     2281 catccgcaga taccgcctga aatgctgttt gcggaggtgc ttgatgcgtt aaccgtgcca
     2341 tagttgcggc agccgttgaa ttccgcaaaa ccgtattccc tgccgacaat tcctcctacg
     2401 aatacgccgt cagtgcttcc cgaatttgag aaaatgttgc cgtagttgta gcaatttatg
     2461 tatttatgat gttgagtcga tgagccgtgg ctataaaggt tacctacaat accgccgaca
     2521 tcgttacccg aaacagctat gtttcctttg ttaatacaat cgcttatgat aacagtaccc
     2581 gggccatcaa gattacccat gtaaccaagt attccgccga ttgccgaaac accgtttttg
     2641 atatcaccag tatttacgca attcgtgatt ttccatgtcg ggttgtttgc ctgactctct
     2701 acagtcgaaa gaatacctgc accgtttgat tggccgtttg ttgaaactgt acctgtgtta
     2761 aggcagtttg taatctcaca gccgtcgtcc tgaatccatg cgcaaagtcc tgctgtatac
     2821 tgtgcgccgc tgatattacc ggtattgaca cagttgttca tcatggtctt gcccttggcg
     2881 tagccgacga tgcctccgac ataattgttt gtaggtgatg aaatgcttac tgtattttta
     2941 cagttgttga atgttatatt gccgtcgtta ttacttgcgc ctacaatacc gcctatatac
     3001 cacgttttac ctgttatcgt accgctatta gtacaattct cgaattttga actgttaatt
     3061 ccttcaattc cgccgatgat accgccgata aagtcattat ttgcggtaat tttaccttca
     3121 tttgtactgt tagttatggt aaccgtattg cctgtcgctc ttccgacgat gcctccgaca
     3181 tacttatcac ctgtaaccga accgtaattt ttacaatttg aaatattaaa tacggtgttt
     3241 tctccggaat ttccgataaa accgccgcaa ttgttttgag ctcttacttc tcctctcata
     3301 atggaatttg taatattcgc ttcggcattt acaatatagc cgataaatcc tccgccgctt
     3361 tcaccactgc aaacggttcc ctccattata acattttcaa ttgtagtttt acctgatgtc
     3421 cacccgataa gtccgccgtg gtcggaatcc ttttttgcca aattcttatc ataaacaaag
     3481 gttgtcgaag caacactaac catcggcgta tcatttttta taattatttc ggccccgatt
     3541 gattcaccta cgaatccacc ttctttattg cctcgtctat tgtcatcttg attcggggaa
     3601 acctttgtgt tgattacttt gcaattggta aatacagttt ttttaaaggt ttgacctaca
     3661 atgccaccgc atccgtagcc gtaatcattc tctcttccat ctcttccgaa tttaataatt
     3721 ttcgctccgt caaccgtaac attattaatc tgtacgtccg tataagcagc gctgctttcg
     3781 ccctcaattt ttccaatcag agcgcctaat ccgtacacgt ttgttctgct tttgtctcgt
     3841 ctatttaaca ccttaatata agtgtcctta attgtaagat cctttacaat gccgccgctc
     3901 atttttctga taacaccgaa gccgccaaca tttgttcgct ctgatttgtc tatactctgc
     3961 ctataattgg ctacaatatg attagcaccg tcaagctcgc cgtaccaagc cgttgcggaa
     4021 tcatcccaat acgcatatgt tattccgcta aaatcaacgt tacagtcaag aacaaccttt
     4081 ttaccgttaa atgtggtcgg atttttaaga aaatacgcga atccgttacc tgtcttgatt
     4141 gtatatgtac ttcctgacag tgtgtagtct gtgccggatg tccatgtacc gtttccattt
     4201 gtaaccttcg aataaacaac atcagccgcc aaagccttcg gaagaagctc cgaaagtaca
     4261 ctcggttcaa agataaagaa cgtcgtgaac atcatgacaa ggcacaatgc cacactgatg
     4321 aattttcgtc cgcaacgggc gaaggatcga ttgattttca tgtttttcac ctcataaaaa
     4381 attcaaatca attttcactt tcaaaatctt gttttgaaat ttattatcgg gatgcatagc
     4441 aatcccagtt tgctatacat tataacacgt ttatattctt tattcaacct taaaaaaggt
     4501 tatattttcg tcaaaacata gattttttgc ctttttcccc tttttctttg ttaattatca
     4561 cgaaatacgg tcgttatcaa aaataacagt tttgagtcgg attttatcgg cttgccgttc
     4621 tttttctgtc tgcttctaag aatatgcaat gaataatatn nnnnnnnnna gcaaattgtg
     4681 attgacagcg ccatgtgaca caagcggttg tatctttgaa aatgatgtgt aaaatataat
     4741 ttttctgttt aaatttctgc cgtaaaagtg tataatgtta tggtaaatgc aattattgat
     4801 gtaaaaggaa gtttataatg tcgtatattt ttaattcctc tgttctcggc tccatgtttg
     4861 ccgtgccggc tcaggtcgtg gataactaca taaaatttgc gagcgcttct cagctcaaga
     4921 ccctgctttg gatatgtcga cacatttcag agccgatcga ctccgccaaa atatcgaagg
     4981 aaatcggata cagcgtcagt gacgttgaag atgcgctgac cgtcattgca agctggggag
     5041 ttcttatcgg aacggatgnn nnnnnnnnga tgtttccgct gctcccgctc ctattgctgc
     5101 cgaagagccg gaagcgacgg agccgccgaa gcagcttgag gaaatttccg ctttaaagcc
     5161 ttcaagtgaa cagataatga cacgctgcaa ggaggatccc gaaattctcg caatgttctc
     5221 tgacattgaa aagatgctcg gcaaaactct cggttacgac agcagaagca ttcttctaat
     5281 gatgcatgac cactacggtc tgccgattga ggtaatttat atgcttgtcg attattgtaa
     5341 atccgtcggc aaaagcggat tttcatacat ctcaaaggtc ggcaagactt ggggtgaaaa
     5401 ggaaatcgac acgatcgaaa aagcggacga gcaaataaaa atcctcaact cctgtaacaa
     5461 gctttggaaa gaattcgccg aaatggcagg aattcagaat cccagaccgt cctcgtcgca
     5521 gtcggcttat ctcagaacat ggtcggtcga gctgaaattc aacgttgaaa tgatttatct
     5581 tgcatatgaa gaaatgctca accattcgag caaaataagc ttcccgtaca tgaataaaat
     5641 cctgatgaat tggcactcca agggaattaa aacccctgac gatatcgaaa aagaaaaaga
     5701 agaataccgc aggaagaaat cacctgttgc gaaaaagact gcatcaactg catcatatga
     5761 catcaatgaa tttattaaac gaggcgaagc attgccggta tataagaaag gaagctgaac
     5821 atggcataca gcaaggaaac atataaaaaa gctgagcagg agctggcaca aagaagaaaa
     5881 cgtgcgcttg cagagcgtga ggaacaccac agaattgccg ttgaagccgt tcccgaaatt
     5941 cttgaggttg aggaaagaat gtcctctgca ggcttggcaa ctattaaagc tctcggcatg
     6001 ggggcagcgg acgcaaagga atacataaaa aagctttcaa aaatcaatct ttccgcacag
     6061 gctca
//
LOCUS       DAWBSW010000122         6061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2481_length_6061_cov_2.458541, whole genome shotgun sequence.
ACCESSION   DAWBSW010000122 DAWBSW010000000
VERSION     DAWBSW010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_6061_cov_2.458541"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    287..363
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2826..2835
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1635 a         1148 c         1411 g         1780 t
ORIGIN      
        1 ctccgtagct tatattataa acaggtttgt ttttcgcggc aaaaataaaa ttaaatatac
       61 ggtgattttg cttgcgtttt ccgtaagtgc agtgattatt ctatactgtt ttagatttta
      121 tccttcggcg cctttcggaa ttattcaagc cgtcaatgct tcgccgtatt tcataatcgg
      181 caggtttgca aagcagttga tttataagga cggcaaggta aaagctgcta taccgatttg
      241 ggcaaaggtt attgcttctt taattttgat tttaggcgga gcttttnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnaagaacc gattttgcaa tgaacgattt cggaaagctg tggatttata ttcctaccgc
      421 tgttgtcgaa attttcggat ggtgtttgct tgcaagtata ctccgttccg ccggattggc
      481 atatatcgga cgacacacta tgccgatact tctaatgcat aaattcccga ttcagcttta
      541 tggttttctt ccgatttcaa ccgagaatac gattatttct attcttttat ccttttgcac
      601 ggttgcactt tgcctcgctg cggcatggat ttatgataga ataataagca tctttacaaa
      661 tgataagggc tgatgctcat tcggcatcgc cctttttcat ttatttcctt gacggcggaa
      721 tacatattgt gatagaataa aacacatatc aacaagttct aagggggata tgtaatgcaa
      781 gattttaaag aaagcggatt tatgtacgga cttgcggcaa tggttattgt tttcgtcatt
      841 gttcagtcct tgttttttct tatccgtgca tggaagcatg gcaaggaggt tggtcttacg
      901 accgcagaca tgaaaaaggc ggttacttca agtgcgctgt ttacgatcgc tccggcggtt
      961 gctatcttcg cgacggttat tacactgtcg agcacactcg gaattgttct tccgtggata
     1021 agactttcgg ttgtcggcaa tctccagtat gaggttaccg ctgctcaagc ggctcttgag
     1081 tccttcagag acggtacaag tattgctgtt ttcggtcaaa acatagccga tccgaaaata
     1141 tttgctgccg tttcatgggt tatgactatc ggcgttattc tttcgcttat acttattcct
     1201 gtttttctca agaaaattca gagcaaggtc ggcaaggtaa ctaacaacgc aagcggtgaa
     1261 aacaaattcg gcgatattat ttccgccgca acatttatcg gtcttatttc tgcattcatc
     1321 ggtaatgcac tcgcaggcac agctccgaca atcaaagaga acggaaagct taagcctgtt
     1381 ggagtgggcg caggtgttat gtcggttgcg gttcttatta catctgttat cgttgttgtt
     1441 gttcttcagg ctctctgcaa gaaattcaag tgggataaat ttgagccgtt tgtaatgccg
     1501 ctcggtatgt tcgcaggtat gggaatggca gtccttctgc attatgttct tcccgcggaa
     1561 atatcaacgt ttgaatggag gccgatcgta tgagcaagaa aaaacgcagt tatatggact
     1621 ccacctatac gtggggaaat attttcatgg ttacggcaat ttgtgttttg gttgccgttc
     1681 ccgtcgcaat atgtttgaaa tttaacgcat ggccgacatt taaacaggta tttgacggat
     1741 tattaaaggt tattcctatt ttctggacgt ccgcaatttt ggaaattgtt gtttacgctc
     1801 cgatgctcgg cacgggcgca acataccttt cgttcgttac aggtaacatc acaaacctca
     1861 agcttccgtg tgttctcaac tcccgtgagc ttgcaaagac gaagcccggc acagaggcgg
     1921 acgaggttat ctcaacaatc gcaacggcga catcgtctat tgttacaacc ataattcttg
     1981 cggtcggcgt tcttgcgctt actcctattc ttcctgctct taccggcgag gattcggcac
     2041 tgaagcctgc ttttgaccaa gttcttccgg cacttttcgg tgcgctcggt gcaggatacc
     2101 ttgcaaagca ctggagaatt tcttttgttc ccgtaatcgc tgcggttatt gtgcttatgt
     2161 tcgacggaag tctcggtacg ggtacactta ttccgatttg tgttgttgtt tcaattcttg
     2221 cagcttatct tatgtatatc agcggattcc tcacaggcgg cgtaaagccc gaaaacccgt
     2281 taaaaaggaa gaagtaaaat gaagacgaca acaaaaagaa attatgaaaa tactcttttg
     2341 atctttttta tttacgcact gttatttatt gtttttctgt ttaatttttt tgataccgat
     2401 gattattcaa tgcagacctc atattcggct ttatcgattg caaacggtct cgacttttct
     2461 attcattacg gcaacggaag atttatagga aatcttgcac tttattattt caatttttat
     2521 ccgataacaa gaatggtttt taagccgatg gttttaactg tattgatcgg ttgcataatt
     2581 tatgttttcg agattaaaaa actttggctg aaaattgcaa cggcgcttct tattattatg
     2641 ccgtcatcgg gcttttatgc caagtgctat tcttcaaacc cgtgtatcat gaattacgtt
     2701 gcgccgcttg cgaatgtttt tgtttgtttt gctttgatga aaataatcgg ccgcaagaaa
     2761 aagaaaatga ggttactttt gtataccgtt ctttttattg catcaatatg tatgcagttt
     2821 tacagnnnnn nnnnntaatg atcttgacga gcagtttaag caaactgtgc aaaatgcagg
     2881 agacaaaaaa atcttcttcg gcgggcggtt tgcctataaa tatttcaccg agaaaaaatt
     2941 tgaggcagat catattatat tttttatcgg aggaattata ggcgcggctc tgatgtttat
     3001 tgtgcctaag cacataagct acagcgttat aggcgaaaac gtcatgtcga gttatcggca
     3061 gataattttt aatattccct ttgcaatagg agttgtggca aagtttgcgg agtatttcag
     3121 cacggcggcg atttggatcg ttatttttgg tgctgtctta acgtatattg taataaaaga
     3181 atcgccggac gataaattta agctttggca catcgtcttt tcaaatgtat atccggcggt
     3241 atgcgttctt tataagttta ctcaaacaga ggaagcaaag gtaatttcaa gcgttaagct
     3301 cttccttatg gtattgatgt ttgcgtttct tcttaatgct cttataatta tattcaggtt
     3361 tattaaggat ataaagacaa aatcttattc tgtttttgct atatttgccc ttgcgatgtc
     3421 cgtaggaatg ttcatgattc ttactctcca cggctacaga actttttatc tttccctgat
     3481 agtatttatt tcgcttacaa tttatcttgc aagctacatt gtaaatgaat acggcattag
     3541 gcttgaagga aaggtcttgc gtgtgctcaa tgttacagct gtttcggcgc tgctttgttt
     3601 tgcggttttg cttccgcttc aaacgataca gaattatgac gtttttgcaa tgaggcagga
     3661 atatgtcgat gaaaaaattt cgcagggaga aaagcttgtt tatgttccta aggttccgaa
     3721 caagaacctt gtaagagatg aatttctgca tttctataaa gattacttta ctgccgaaca
     3781 tcaggacgtt gacatcgtat ttattgatat tgaggattgg gagcgatttg aagaatatca
     3841 gtcgatgatg gataatccgc tgacctctat aacatacgcg gtgagtcatc ttcaattcag
     3901 caactgaact ctgaaaaaga aataataaaa aacacttgat atttacatta taatgtggta
     3961 ttatatatct gtaagtttat taaggtattg acaaaggagt ggggcgaccc gctcctttgt
     4021 attgtaaagg aggcgttaca atggcttcaa aaggaaaggg cggaattacc gtcaataccg
     4081 tttgggatat cgctcagccg attgccgaga gtcttggact tgagctttgg gatgtacgct
     4141 ttgaaaaaga gggtgcagac tggtttctca gagttttcat cgacaaggac ggcggtgttt
     4201 caatcgacga ttgcgttgac atgagccatg ctcttgataa gccgcttgac gatgctgacc
     4261 cgatcgaaca gagctactgc cttgaggttt gctcccccgg actggagaga aatctcaaaa
     4321 gggacagcca ttttgaaaag tgcataggca agccgattca ggttaagctg attcgtccgc
     4381 ttgagggcag cagagagtac aagggaactc tcgagagcta cgacaacgga aattttgagc
     4441 ttcttactca ggatggggcg aagctcgtta taaataaaaa agaaacatct tatgttaaac
     4501 ttgatgattt cggaggagaa gaacaatggt aaacaaagag ctttttatgg cgctcgaaat
     4561 gcttgaaaaa gaaaaaggaa tacctatgga tgtactttgc gagggtattc agaaggcgct
     4621 cgtcacggca gttaagcgtg actacaacaa caaggacatc gttttctgcg agcttgaccc
     4681 cgagaaaaag acaatgcgag tttttgtaag aaagaacgtt gtctccgagg tggttgatcc
     4741 cgatgaggat ttgaccgttg aacaggctca gaagtataag ccgacagcta tggtcggaga
     4801 cattattgaa attgagcttg agacaaaaga ggtcagcaga attgcagccg ataagggcaa
     4861 gcatgttatc cgacagggta tccgcgaggc cgagcatggc agactgcgtg aggagttcca
     4921 ggctcacaat caggagatcg ttacagccaa ggtttcgcga gttgatcccg agactaagga
     4981 cgctatcgtt gaaatcggca aggctcagga gccgctcttc agaagcgaac agatcccgaa
     5041 tgaaacactt cttccgggta tgcttatcaa ggtctacatt gccggagttg ccgatgcaaa
     5101 ggaaggcaga agaggaccga gagttacaat ttccagaacg catcccggtc ttgttaaaag
     5161 actgtttgag tccgaggttc ccgaaattta tgacggaacg gttgaaatca aagcagtttc
     5221 ccgtgaggca ggttcaagaa caaaaatcgc cgtttattcg gcagatgaga atgttgacgc
     5281 agtcggcgca tgcataggcc cgagaggagc ccgtgtcggc aagattgttg acatgcttta
     5341 cggcgagaag atagacattg ttaaatacag cgaggatccg aaagagttta ttgccgcttc
     5401 ccttgctccg gcagatgttg tttcggtaac aatagacgag aacgcagaaa agtcgtgcag
     5461 agttatagtt ccggatcttc agctttcact tgctatcggc aataagggac agaatgcaag
     5521 acttgccgct aagcttacgg gctggaagat agatatcaag cccgagagcg gaaaaactga
     5581 cccgtcggta gagctttaat taaaaggagg tgcaggaatg cagcagaaga aaattccgat
     5641 gcgcaaatgt atgggctgcg gcgagatgaa gccgaagaaa gagcttgttc gtgttgtgaa
     5701 aactcccgaa gatgagatag ttcttgacct tacgggcaga aagaacggca gaggagctta
     5761 tatctgccat gacgccgaat gtcttaaaaa agcgagaaaa gccaaaagaa tcgaaaaatc
     5821 attcctgtgc cggattcctg acgaggtcta cgataaaatg gcagaggagc tgaaaaacga
     5881 tgaatgattc ggcgctcaat cttttaggtc tttgcagacg cgcaggcagg ctcagcttgg
     5941 gccatgatgc ttgcaagcag gcgataaaca gcggaaaggc aaagctttgc atagtttgtt
     6001 cggacgcatc aaaaaggctt tccgatgaaa tatcggggct tgcaaaggca cataacaaaa
     6061 c
//
LOCUS       DAWBSW010000123         6005 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2495_length_6005_cov_3.172941, whole genome shotgun sequence.
ACCESSION   DAWBSW010000123 DAWBSW010000000
VERSION     DAWBSW010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2495_length_6005_cov_3.172941"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    474..483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    863..872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1786 a         1248 c         1287 g         1664 t
ORIGIN      
        1 aatgcaaaca gcggtcaacc tttttctgcg gcttttcctg cgttcgccgt tcatcgttat
       61 cggcgcagtc gtcatggcct tcacggttga tccgaaaacg gctataattt ttgccgtttc
      121 gattcctatg cttgcggttg tcgttttcgg catcatgttc tattctgtgc cgatttataa
      181 gaaagtacaa aacaggcttg attccgtaat gaggatgacg agagaaaatc tttcgggcgt
      241 gcgcgtgatt cgtgcattta accatgagca aagggaaatc gaggactttg acaaatgctc
      301 ggaggaattg agggatatgc agctttacgg cggcaagatc tcggcatatc tcaatccgat
      361 aacttatgtt atagtcaatc tttcaatcgt gctcattatt tacgtcggcg gacttcgtgt
      421 agacacgggc agactgacgc agggcgaggt tatttcactc atcaactata tgtnnnnnnn
      481 nnnaatttga ccatgtaagc gcaacatatg caggatcaaa tgaaaaatcg cttgactcgc
      541 tcacgcttaa cgttccgaga ggttcgacgg tcggcataat cggcgcaaca ggctcgggta
      601 aaacaactct tatcaatctt ataccgagat tttacgatgt cagcggaggc tcgctcaagg
      661 tcaacggaac ggacgttcgc aattacaatg ttgacgagct tcgcaagctg gtaggcgttg
      721 ttccgcaaaa ggcggcgctt gtcagcggaa cggttcgaga caatatgaaa tggggaaagc
      781 ccgatgcaac cgatgaggaa ataaatgcgg cactgaaaac tgctcaggct ctcgactttg
      841 ttgacgaaaa ggatggactt gannnnnnnn nnattcttca aggcggcaag aatctttcgg
      901 gcggtcagat tcagcgcttg acaattgcaa gagcacttgt caggaagccc gaaattctca
      961 ttcttgatga cagctcgtcc gctcttgact ttgcgacgga cgccgcactc agacgtgcga
     1021 taaagtcgga cacggataat atgactgttt tcattgtttc tcagcgtttt tcgacaatca
     1081 agaacgctga catgataatt gtgcttgacg atggcagagt ttgcggaatc ggcaagcatg
     1141 acgagctttt tgaaagctgc gaggaatacc gtgatatttg cgaatcccag cttttaagca
     1201 aggaggcggt ataaatgaag aataataaca aaggtacatt ccgccgcctg ctcaagctca
     1261 ttgaaaaata taaatttctt gtacttcttt cgttgctttt ggctgttgta gtcgttgcaa
     1321 ctcagcttta tgcaccgatt ttgttcggta atgcggtcga tcttgctctc ggaaagggtc
     1381 aggtagatat tgaggggatt attgcaattc tcgctaaaac cgcagtaatt atcggcataa
     1441 cggctctggc tcagtggata atgaacatca taaacaatcg cataacatac agggttgtta
     1501 aggatctgcg tgttcaggca ttccgccatt tgcaaaaact tgacatatca tatatcgact
     1561 ctcacgagca cggagatatc gtcagccgaa ttattactga tatagatcag ctctcggacg
     1621 gtctgctgat gggctttacc cagtttttta cgggtgttat aacgattctc ggcacattga
     1681 tttttatgtt ttccgtcaat gtaaaaatcg cgcttattgt cgttctcctc acgcctcttt
     1741 ctcttttcgt tgcttcgttc attgcgaaaa gaacctactc gatgttccaa aagcagtcag
     1801 aaacatgagg cgaaattaca acgcatatca acgagatggt cggcaatcag aagcttgtca
     1861 aggcattcag ccgtgaggac gcttcggaag aaaagtttga cgagataaac gacagactca
     1921 caagatacag tcttaaagcg tcgttcttct cctcaatggt caatccgtca acaagactta
     1981 tcaatgccct tgtttatgcg ggtgtaggcg ccttcggtgc tctctcagcc attgcaggca
     2041 gacttacggt cggtcagctt agctgcttcc tcagctatgc aacgcagtat acgaagccgt
     2101 tcaacgagat ttcgggcgtt gtcacggagc ttcagaatgc cctcgcttcg gcggcaagag
     2161 tctttgaact cattgacgaa aaggaacagc ttcccgactg ctacaatgcg aaggagcttg
     2221 aaaaggcaaa cggcgaggtt gagtttgaaa atgtttcttt ctcatacgat aagagcaagg
     2281 agctcatcaa aaatctcagc atcagtgcaa agcccggtca gcgtgttgca atcgtcggac
     2341 cgacgggctg cggcaagagt acgcttatca atcttcttat gcgtttctat gatcccgaca
     2401 gcggaatgat caaggttgac ggcgaaaatt caatggattt cacaagaaaa tcattgcgta
     2461 aaaacttcgg catggtgctc caggaaacat ggcttaaatc gggcacggtc agggagaata
     2521 tcgcatattc aaatcccgac gcaacggatg aggaaatcat tgccgccgca aagcttacgc
     2581 acgctcacag ctttataaag cgcttgcccg acggctatga cacggttctc ggcgaaagcg
     2641 gcggttctct ctctcaagga caaaagcagc tgctttgtat aacgagagtt ttgctcaatc
     2701 tgcctccgat gcttattctc gatgaagcga catcgtcaat cgacagccac accgagatta
     2761 gaattcagaa aacctttgcc cgaatgatga agggcagaac atgctttgtt gttgctcacc
     2821 gactttcgac gatccgtgaa tcggacatca ttcttgttat gaacaagggt cagatcatcg
     2881 agcagggcac gcacgaacag ctgcttgaaa agcacggctt ctactatgac ctttacaaca
     2941 gccagttcgc cgtgacagaa taacaaaaca aaaatttaac cgttcttttt tgtgcgtggc
     3001 ttcggctgcg cactttttgt tttacgttac aaacagttga aaaatatctg tcaatatgat
     3061 ataatacagc taccacacaa agttcatata gaagctaaac caaattgcgt agctggaata
     3121 gataaaaata ccggcttact tttctatgct tttggtttag aactgtaact ttgtgtggta
     3181 gcaatcaagc cgtgtatttt ttgtgcgtgg cttcggctgc gcacttttta tttttcgggg
     3241 gataataaaa tgattgatct ctatgactat gtctttaact cagaggaaga aattccggaa
     3301 aaggttgttg atgaaacggg agcggtgctg actccatgtc accccgaaac ctgtgagggc
     3361 aacggtatgc actccgacca tgaaatttgc tgtgatgagt gtaattggtt tctcggctgt
     3421 tttcctgaat tctgcgatcc ggattattgc ccgacagaag aataaaataa aacacaaaag
     3481 ctataattca cattcagctt gttgaaatcg ctgatatgcg gattatagct tttcgctttg
     3541 ttatgtcgga ctgttcacat taagggtgaa agtcgtttta tttaacctta tttctgcttg
     3601 ttagcaatga tatccttaat gaacttcggg caaacgatat caataattct gccgatccat
     3661 gttgcgataa caggtgagaa gagatccatg atgtagtaat ttatcggctt agatgcaaca
     3721 tcattatatg ttacaagatg agtttcgtaa ttccttacat cgttctcgtc aatctcaaaa
     3781 attcttgccg aacggtcgcc gcctctgccg tatgtgctga agcctgaacc gccgttgtag
     3841 ccgagagtga ttccctcgtc cgtaacgccc atgaagttgt taacgtggtc atgtgcgaag
     3901 aaagcaccga taatctcttt atgctcaagc catgcttcat actcgcctgt gttgccgttt
     3961 acggattccg aacacggaac ctcaccgaga acgccgcttg tagctttatc gttgagcttg
     4021 taccactgac cgtcgtcaag accgaagatg cagcctgtat gatctgcatt gaacggaacc
     4081 ttatcaacaa actgatagat ttccttaacg ggaacatgct ggaaaacaag agaaggaaca
     4141 tagctgcctg tagctgcctt gagtccgtca cgggtatcct tataccactg aacctgctca
     4201 ggcttgagtc cctgataacc tgtaagaaga ccttcgccct taaccttatt gttggagtcc
     4261 ataacataga tgttaagagg aattttctta ccgtcggacg acttaacggg aatataaaat
     4321 gtacctacat cacagccgtt attaacaata gcgcagttgg aatatgactt gtaaacctcc
     4381 atctgctcct caagcgggaa gatctcctcc cagtcatggt cgtggttgcc gaaggtagct
     4441 acgaaaggta ctccgagagc gttgacgccg tcaagagcct gagtaaggca cttcttgagc
     4501 tgagctttgg ttgcgcccgg gaagaagtct gtgagaaggt cgcctgccat aacaatgagg
     4561 tcgggctttt ccttttcggc aatcttcttg aggtagcttg cctggagaac gtccttgaga
     4621 ataacatcct gtgtgtcgtt gatcatcatg atcttaaatt ttccgtctga gttgaacttg
     4681 aatgccgtgt cggtgctgat cgccgcaaag ctcgggacag caaggaggac aatcataaca
     4741 accgagagaa gaatacttat gacttttttc attgttttaa cctccgaaaa aataattttg
     4801 tagcttaatt atacaccaac aaaaatttaa agtcaacata aaggtttaga tgcccatgaa
     4861 aaattatgac aaatcacaca aaaagatgta tcaaaacaac atgctgtata atacgtcatt
     4921 atatcaatct ttaatatgtt tttttatttg ccattttatc gcatttataa ttttactgaa
     4981 cagcttacaa atcaaattaa taaacatatc aatatgcaaa agcctaaaaa gttgaattcg
     5041 cagggaatca acaagcgaag ctgcaagata aaacataact gaaactccga gcacaatacc
     5101 aatcttttcc aacgcagtcg gagcattaaa aaatgtgtgt aatttagtaa taatgttatt
     5161 ccatgtgaat gggagacatt ggattatata tacactaaat gacgtggatg aaaaaaacct
     5221 gattatcctc ttgcctcgtt tgatattcat tctgctgaaa agcatcagaa aagagataga
     5281 aggcaataaa acagttaatg aattgtattt ttctaaaata tcttttggcg aataattgat
     5341 aaatatattt atattatccc aaacaggcat ataacagcaa tacgttatca acattccaac
     5401 aataattatt acgacatata tacgattaga gatactgttg aaaaaattct cgttcttttt
     5461 tataaatgct ccgataagat aacaaaggga aagccataaa aaactgtatc cgtcaagcaa
     5521 accaagaatg cttatattgg ttggaataaa taaaatagca aaacaaatta ttatataatt
     5581 aaataaaaag ccatttgcaa aactgcttat aatttgatta tacagcggaa taaaaaagaa
     5641 cataataaaa tatgccgcga aataccatag attatctgaa attgtaacag gaaaagatgc
     5701 gctcaacaca tttaaaatat tgataggtct atgaaatatt tgtttgtcaa gtatcaacaa
     5761 tatatatgaa taaaaaacaa cttgaagcca taaattaata atttttgtgt atttgacggt
     5821 ggaattatac ataacaaaac cggaaataat tgcgaaaatg tccactgcac catagcatat
     5881 aaccataatc aaagaaagca catagtatga cagtccgttt tcaggggtac ctaaaaatcc
     5941 gccatatccg gagaaatgta atatcgtgac cataatcata gaaactattc tgagcagatc
     6001 tatac
//
LOCUS       DAWBSW010000124         5958 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2510_length_5958_cov_2.452143, whole genome shotgun sequence.
ACCESSION   DAWBSW010000124 DAWBSW010000000
VERSION     DAWBSW010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_5958_cov_2.452143"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3127..3136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5430..5439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1722 a         1269 c         1552 g         1395 t
ORIGIN      
        1 caattgacaa ggtgtacaaa ttgcacaaat tttagcttta aattatgttt aactttaaga
       61 attaaatctg tgactttttt ataaaactgt gttataatac taagggactg aataaatttt
      121 atttactttc aataaaatat cagtcggaag tcggaggtaa tcggttgttt gagcagatta
      181 taaattttga aagaatggaa caggctgtca gtcttttcgg cagcttcgac gaaaacataa
      241 agtacataga aaagaaatat tccgtctcgg tcgtgtgccg aggtgcggaa atgaagataa
      301 gcggcgaagc cgagaatgtc tcgcttgccg tcagagcggt gaacggcttg cttgatttaa
      361 tcaataaggg tgagtcgctc aatgagcaga atgtcagata cgtcacctct ctcgttgacg
      421 agggcggcga ggatcagctc aaaagcatga acggcaactg catctgcatg acgacgaagg
      481 gtaaacctgt taagccgaaa acgctcggcc aggagaaata cgttcaggca atccgtgaca
      541 acacgatagt tatcggcgta ggccctgccg gtacgggtaa aacatatctt gccgttgcga
      601 tggcggtcag cgcattcaga gccaaggagg tcaacagaat tatcctgaca cgtcctgcgg
      661 tcgaagccgg cgaaaagctt ggatttctcc cgggtgattt gcagcagaag gttgacccgt
      721 atttaagacc gctttacgat gcgcttttcg atatgctcgg cgccgagaat tttcagcgat
      781 atcaggaaaa gggaaatatc gaggttgcac cgcttgcata catgagggga cgaacccttg
      841 acgacagctt tattattctt gatgaggcgc agaacacgac gacggagcag atgaaaatgt
      901 tcctgacccg tctcggcttc aactcgaaaa tggtcgttac gggagacata acgcagatcg
      961 atctgcccga cggaaagcgt tcgggactgg tcgagataac caagatactc aaaaatattg
     1021 aggatataaa aaccgttaaa ttttcagaga aggatgttgt acgccataag ctggtgcagg
     1081 acattataaa ggcgtacgaa aagtatgagg aggcaaagaa aagacgatga gcgataagat
     1141 taaggttatt atcacaaacg atcagaagga aattaagata cccacaggtg tcagaatgtt
     1201 gattcgccgc tgctgcaatg cggtgcttgt taacgagaat tttgaaggct cggcggagat
     1261 aagcgttcga tttgtcgatg acgagatcat ccatgagctt aaccgtgagt acagacacgt
     1321 tgacagaagc acggacgtgc tctctttccc gctcggcgag aacggcgttt acgacatcaa
     1381 ccatgacacg ggcgcaaaaa ttctcggcga tattgtgatt tcaatgcagc atgccgttat
     1441 gcaggctgac ctttacggtc actcgcttca gcgtgaaatt gctttcctta ccgtacattc
     1501 aatgcttcac cttctcggct acgaccacga ggccgaggga cttgaaagag tccgtatgcg
     1561 tgaaaaggaa gaggctgttc tcactcagct cggacttaaa agaaacgaca gctactattc
     1621 ggaggacaac tgatgacaaa aaccgcattt attgccattg tcggctgccc gaatgtcgga
     1681 aaatcgtcga ttcttaaccg tctgctcggt cagaaaattg ccattgtttc ggacaagccg
     1741 cagacgacga gaaccaaaat tatgggcgtt ctgaccgagg gcgacactca gcttgttttc
     1801 accgatactc ccggcttcca cagaccgagg acgaagctcg gtgaaaaaat ggttcaggcc
     1861 gtttcggaca gcgtttcggg cgttgacgcc tgcctttttg tcgttgaacc ggagggtgag
     1921 ttaagacctg ccgagcttga gcttatagaa aaattcaaga agctgaaaat gcctgttatc
     1981 cttgcgataa acaaggttga cacgcttccc gacaaggaag ttttgatggc gagaattctt
     2041 gaaatttcca aaacctacga ttttgaagcg gtcgttcccg tttccgctct cagggggata
     2101 aacatggacg agcttctttc ggaaatgaaa aagcttgccg aggaaagcgt gttcttcttt
     2161 ccggaggaca ctttgacgga tcagcccgag cgtgttattg cggcggagat catccgtgaa
     2221 aagcttttga gaaatcttga cagagagatt ccgcacggcg ttgcggtcag cattgaaaaa
     2281 atgcgtgaac gtgaggactc cgacatcatg gatatcgagg cggttattta ttgcgaaaag
     2341 gacagccata aaggaataat aatcggcaag aacggagcaa tgctcaagcg catttccaca
     2401 agagcgagag aggacatgga aaagtttttc cagtgtcaca tcaacctgag atgttgggtc
     2461 aaggtcaagg aaggctggcg caacagagag ggtcttattc ataatttcgg acttgactga
     2521 taaaaaacgg gcatcaaaat gacggtggat ttttttaccg ccgtgaaaaa taaataaaca
     2581 tctgatccct gcaaataatt ttatttggag ggattttata tgaataacag ctttaaggat
     2641 cttgccgcat gggataaatt cgcatcgagc ggaaagattt gcgactatct tcaatacaag
     2701 catatttcaa cacaggagaa caaggatgaa gtttcggacg gaaggactga taatcaggga
     2761 gcagcagacg ggagaagatg accgtctggt gactctgctt acccgtgacc acggcgttat
     2821 cagagccttt gtaagaggag ccaagaggat aaaaagcagg tcgcagagcg caaccggact
     2881 ttttgcctac ggaagctatt ctatttacag gggcagggac gcctattcgg tcgatgaggc
     2941 tcagccgaag gagatatttt ttgatttaag aaacgacatt gaagctctcg cactggcaca
     3001 gtatttctgc gagctggcgg gggagcttgc tccggttgag gacgacgccg acgaattttt
     3061 gcggctcatc ctcaacgccc tacatatgct gatgaacaaa aaaagaccgc aggctcagct
     3121 caaagcnnnn nnnnnnaact gaggcttctc tgcctttcgg gttacatgcc cgacctcgtt
     3181 gtttgcggcg actgcggcga ggaatacgac ggtaattttc ttttcagccc cgttgaaggt
     3241 cttatcttct gcgaaaaatg cagacacaag cactcgggtg taatgcttac gccgggagtg
     3301 ctccgtgcaa tgagacacat ttgctacagc gaggacgaaa agctgttttc tttcaccctc
     3361 ggcgaggata gcatgaagat gctcggcgag gtgactgaaa gatttctgct gacgcagaca
     3421 tcgaaaaagt ttaaaacact tgatttttat aaagcaatgg caatatcgca aaattaaggt
     3481 gttgccttaa gaaaggatga gcaaaatgaa ccgacagtat ccggcacttg agctggataa
     3541 ggtgcttgag ctactggctc agcatacctc atgtgaggat gctcggcttg ctgctctgaa
     3601 ccttgaaccg cagaccgacc ttgcgtcggc gcaggcactt atgaatcaga cgagggacgc
     3661 acacatgctc cttgcacgct tcggcggtcc cgcattcggc ggccttataa atgttaataa
     3721 cgcactttac cgtgccgatg cgggaagcac gctcagtctc aaggagcttc tcaacgttgc
     3781 gtccgtgctt catgtaataa gaacgatttc gcagtggaga agcaccaacg aaggcgttgc
     3841 aactgtgctt gacgtgtatt ttaatgcact catgccgaac agattccttg aggattcaat
     3901 tacgacggcg atcatctccg aggaggagat tgccgacaac gcttcgccga cacttgccga
     3961 tatcagaaga aagattcgtg tgcaggaatc caaggtcaga gatcagctcg gtaaatatac
     4021 gcacaattca aatttttcga agtatcttca ggataatatc ataacaatga gaaacggccg
     4081 ctacgttata cccgtcagga acgagtacag gggagaggtt cccggtcttg tgcatgacac
     4141 ctcgtcgagc ggtgcaacgg ttttcattga gccgatgccg agagttgagg cgaataacca
     4201 gattaagctc cttaaaaata aagaggagga cgagattgac cgtattcttg ccgaactttc
     4261 ggccaacgtc ggcagcttcg cggattcgat taagaatagc tacgaatgtg ctgtcgagct
     4321 gaaccttatt tttgcaaagg ctcagcttgc ctatgccatg agagcaagcg ttcctcagct
     4381 caataacgag ggcataatcg aattaagacg tgcaagacat ccgctgattg acaaaaataa
     4441 agttgtgccg gttgatatca gtctcggcac ggactttgat acgatagtaa tcaccggtcc
     4501 gaacacgggc ggtaaaaccg tttcgataaa gacgatcggt cttatgtcgc tgatggcgat
     4561 gtgcggactc atgctccctg ttgacgacag aagcagaata tcggtctttg accatgtgct
     4621 tgccgatatc ggcgacgagc agagcattga gcagtcgctt tcaacgttct cgtcgcacat
     4681 gactaagata gttaaaattc ttgaacttgc cgatgataag agccttgtgc ttatcgacga
     4741 gctcggagcg ggtacggatc cgattgaggg tgcggcgctt gctgtttcga ttcttgaaca
     4801 gcttcgtaag caaggcgcaa agattgcggc gacaactcac tacgccgagc ttaaatccta
     4861 tgccttgcag accttgggcg ttatcaacgg aagctgtgaa tttgacgttc agtcactcaa
     4921 gcctacctac aggcttctta tcggtattcc aggaaggtca aatgcttttg caatttcaaa
     4981 aaggctcggg ataagtgacg aaataatcga gcacgcccaa gaacttgttt caaatgaaaa
     5041 tgttcgcttt gaggacgttg tggacaggct tgaacagagc cgtgcaaaga tggaaaaaga
     5101 gcgtgaggaa gccgaaaaaa tccgcgaaga agccaaccgt gagttggaaa aggctcagaa
     5161 gctgaaggcg gatatcgaaa cgctcaggca aaaggagctt gatgacgcaa agagtcgggc
     5221 ggtaagaata acggaacagg ctaagcgtga agcttatcaa ctgcttaacg atcttgagat
     5281 gctaaaaaaa cagcaggcaa aggagaaaaa cgccgccgag atggctcgcc gtgcaagggc
     5341 agttatcaaa aaggatctca atgcaataga cactgcttcc gatccgatag tttctctcgg
     5401 caatgacgag gactatgttt tgcccagacn nnnnnnnnnt gaaaatcggc gatacggtaa
     5461 taattgcaga tatcggaaat gaagcgaccg ttgttactct caaggataaa aaaggacttg
     5521 tcaccgttca gatgggaacg atgaaaacaa gagtcaagga agaaaatctg cggcttattg
     5581 agaagaaaaa gagcgctgaa aagaaacgta ccgttgccac gggcagaatg gaaagccgca
     5641 tgaatatgaa ggctcagacc agcgttgacc ttcggggaat gacggtcgag gagggcatat
     5701 tggagcttga ccgctacatt gaccatgcgt tgagaatggg aataaatgaa ttcacggtaa
     5761 ttcacggcaa gggtacaggt gttctgcgct cggcggtaag ggattatctg aaaaaatcaa
     5821 agtatgttaa aacttcacgt ctcggcacgt tcggagaagg agaggacgga gtaagcattg
     5881 tcacactcaa atagcagaga taaattattc atggcgttca caatgcttgt tttcggcacc
     5941 ttaggaccct tcataaat
//
LOCUS       DAWBSW010000125         5933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2522_length_5933_cov_2.748554, whole genome shotgun sequence.
ACCESSION   DAWBSW010000125 DAWBSW010000000
VERSION     DAWBSW010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2522_length_5933_cov_2.748554"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    787..796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4012..4021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4307..4316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1684 a         1192 c         1288 g         1739 t
ORIGIN      
        1 ctcgatatgg tgatttcact ggcacttgca ttcggcatag gccttttcat ttttttaatt
       61 tataaaaaaa ccttcgccgg cgtaatgtat tcgtcaagct tcggcgtaac tcttgtggca
      121 ctgacaatga tatcaacctt tgtaattctc gccgtaacct cgaatgtggt tctttcactc
      181 ggtatggtcg gtgcgctttc aatcgtaaga ttcagaacgg caatcaaaga accgctcggc
      241 attgcgtttc tcttctgggc aattgccgca ggtatcgttc ttgccgcatg tgcatgtgtt
      301 ctgatactcg gagtcgcatt agcgtttgtt tacagaaaga aaagctaaaa ataaggggcg
      361 gttcaatgta aacggcccct taaagttatt tgataatttt tacatatttc ggatcgtttg
      421 atccgtggta atcatatact acatttgttt tgcgtatccg atatattttt attttgtcgg
      481 attctttgag cagtacggtt cctatgtatt cctttggcat aaatggcgga aattctttcc
      541 tgagtgtgca gtgccaaaag ctcgttttca gtgaaacaag atcacagccg attgtcacaa
      601 tgtatgtgta tttcgaagta tcaagctcca gttttgacgg atctatgccg atatcccgca
      661 agcgttccgg ggatgtgctt atgactccgt tctcataatc gctgacggaa aaccagaaaa
      721 tattttgcat ctcttcgctg atttcctgcg gcttgtcaaa ggaataaacc tcggtaaaat
      781 tatatcnnnn nnnnnntgtg ctcaagaaat attgaattaa gaacaacaat tgtcaaaact
      841 attgcagcta ttataaataa ttttcttttt cctttcatct tttccgctcc ctaaatcaac
      901 cgtcataaac gcccattata acaataccgg tgacggtgag aaggatgcac acatagtgtt
      961 tccacgaaag cttttcttta aggaaaattc tgccccagag gacggatgcg gcgcagtaag
     1021 cggaaataat cggcgccgag agggcaaagt gctcactgtc ggcaatggca tagatatatg
     1081 caaactgacc tgccgtttca aacgctgcgc cgatatattt cggagcttcc tttttcggaa
     1141 agaacttatc cttcttgatg aatttaacat aaatgaagca aagtatgccg cagatgagga
     1201 atgtcagttc atatgaggtg ttggcggcat cctcgtcaag cttttcaaga acaaggctgt
     1261 cggcaaaaga tccgagtgca tcaagcagac agtaaataat cggcaagaga attgcgagaa
     1321 cactttttgt atatttatag ttgcttttgc tctggcgtgc cgctctcagg tcatcatcct
     1381 ctgtcatttc ggcaatgctg agtccgacaa tgccgatggc aacaagggcg attgcaatgt
     1441 actgagcctt aacgagtacg gccattcctg tgattatgct gataatagaa gccaatgaac
     1501 cggatgaatt gcatatcgga gaggaaatcg aaagctcgat aaaacgaagt ccgacatagc
     1561 cgatcgtcat tgacgatatg taaaggaatg agacgggcaa ataggtgagc atggtttgaa
     1621 aagtgaagtc aacgtcgtta aaaataagcg tgtatgcagc atgaaggccc ataacaacac
     1681 cgactgcggt gaccatcttc agtgcgctgt gtttatcctt tgagtcggcg cagccgattt
     1741 ttgaaaataa atccgaaaag ctccagcaaa gcagagctgc aatagaaaaa ataaaccatt
     1801 tcataataat aacaacctca atatactaat tttttttcaa catatagcgt cccgtcggca
     1861 aggctgattt tgcctaaccc gaggaacttc tttgcgggag aataaacacg gcagaagccg
     1921 tcgttttttt cgcccttgat tctgttgagg tcgagttctc cgccgttttc aaagcgtatc
     1981 gcctgttttt cgctgacatt gacggcagtg tagcaacgaa gcatttcgtc acacggggtg
     2041 atcagttcgt caattcgtcc ttcgtcggca agctgctgaa cggtttcaag tgtaacggtt
     2101 ttgtctattg aaactccgtt ggcttcaatg cgtctgagtg cggttatcgt ggccccgcag
     2161 ccaagctcgg aaccgatatc ggcggcaagc gagcgtatat acgttccggc actgcatgag
     2221 acctcgatct caaactcgtt attttcgtcc ggaacgtcaa taagctttat atagcgaata
     2281 acaacattcc ttgcttctct ttctatttcg atgccctgac gggcaagctt atataacctg
     2341 acgccgtcct ttgaaacggc ggaatacatc ggaggaagct gacttattga gccgacaaaa
     2401 cggttgatta ccccgttgag tctgtcaacg ctgacgttaa caggattttt ctgaagcacc
     2461 gtacctgtta tatcgagcgt gtctgtgacg gtgccgagta aaacttttgc acggtaagcc
     2521 ttgtcatgag tcggcaggta ctgtgaaaat ctcgtcgctc cgccaagcat gacaggaagc
     2581 acgccggttg ccattggatc gagtgtgccg gtgtgtcctg ttttatttat tttcagtatt
     2641 ctcttgactc tgtttgccgc aacaaaggac gtaagcccct caggcttgtc aagaaaaatg
     2701 aatcctgtca aaattatccc tccgtgagag catagtgcgg ttgaattata cattatagac
     2761 gaaaaaaagt caaatattat ctatgtatca gagcgattgt cacattgaaa acataaaaag
     2821 tttgtgcaat taaaatattt gtgtcaatat aagtacaatt ttaattgtta aaacaaccaa
     2881 aaaatatctg ttaaatttgt tacaatcttc aaaaattgag aaaacaacgg attttttgtt
     2941 gacattgatt aattaatgag ataccatata aacgtatctt agtcctgtga ggtgtgcgta
     3001 tgagcaaaat tccgataaat tcggataaag tttatgcggc acgttacggc gataaagcag
     3061 caatgaatga actgatttca tctcttgcgc cgactgttga gtgcattgcc acaggctatg
     3121 tcggacggtg tccgatgagc cggagcgatc ttattcagga gggcatgata ggtcttctcg
     3181 gttctgttta cgggtacgac cctgatgagg gcgtgaggtt tgagaccttt gcaacggttt
     3241 gcatttccaa tcgcatcaaa agcgccgtgc gaaatcaact gcggagcaag catatgccgc
     3301 tcaacggata tgttgacatt gacgacgttg aaatcagcga tgatatgtct gacccgcaga
     3361 cgatgataat tatgcaggaa cggttcgagg atctcagcga gagtatcgaa aaaaagctga
     3421 cggatcttga aaaagatgtg ctgaggctcc atatcggagg tcataattac agttctattg
     3481 ccgaaatgct ttcaataagc gtcaaaagtg ttgacaatgc tttgcaaagg gcaagaaaaa
     3541 agctcaagga aaaatgatac ccctatgcct gcgctgtaca taacgtttaa ggacgtgcgc
     3601 agagcgtaaa taaaatttag cgaggtaaat tatcatgtac gaagacaaga cgttagtatg
     3661 taaggattgc ggtcaggagt tcactttcac cgcaggcgag caggagttct acgctgagaa
     3721 aggctttgtt aacgagccgc agcgctgcaa ggcttgccgt gatgcaagaa agaatgcagt
     3781 cagaggcgag cgcgaaatgt tcgaggcaac atgcgctaac tgcggcggcg tagctaaggt
     3841 tcctttccgt ccgagagaag accgtcctgt atattgcagc gagtgctttg caaagatgaa
     3901 ggaagagggc taattaccga ataattgagg agctgtatca gagttcgtaa aagcgaacaa
     3961 cggtacagct ccttttttgt tttaagtcga aattcacaga ttgccgtgaa tnnnnnnnnn
     4021 nccttaagaa tttgaaaagc ctttaagaaa atcatctacg tcaaagcttt caacgttttc
     4081 ctttgtcata tcgggttttg aagtcttagg cggattatat tgtcccttcg gcttaccgaa
     4141 atccttaaga taactgtcaa tttcctcctc ggaaagctcc ccgttatgcg gcaaggcgga
     4201 ttgaacagtg ttttctttta taatctcttt gaattgactc gggcttatct cggctttatc
     4261 gttgaaagcc tcagcgttaa ttacttcttt ttcctcatgc gtttcannnn nnnnnncatt
     4321 ttttgtattt tcttttgctg cgacggttga ttttttttcg cttgtttttt cttcgacgga
     4381 tttggctgaa aaagcttttt tacaatatat tgcaaccaaa gcaagggaaa taagctgggt
     4441 tgcaagcata tggatcataa agagccattt ggtgctttga tctacaatat ggaggctgtt
     4501 tgcaaggaaa acataaagaa agttgtcgat aatcgaaata tataccgctc cgcttacttt
     4561 tgcaagcatg ccccatttca agcccgttat aaactcgtga acaagataaa aaataactac
     4621 aaatgctgca agctttacaa tatcctcact gtagtagttc atggcaaaat ttcgtatcaa
     4681 tttcggaata acgaatgtca tgtaaagaag tgcctgaaaa atgtttgcta ccttaagggt
     4741 cgtaagcttc tcaagcttgt ggagcagaaa tccgcggaaa aacatctcct tgaagcaggc
     4801 ggcaaaaaga gaagtcaaaa tataaatcgc aatacataaa gccggtgtga gatatccctc
     4861 ggtttgcaca tagctgaggc tcggcggagt gaaattgata tcaatggcat ttggatagat
     4921 tatcttaaaa aatatatatt caaatattga aacagttgca atcgggacaa cggaaaaaaa
     4981 tgtgccgaaa ataaatgcgg atttcagttt agttgctttt ctgcgtatac caaaggttat
     5041 gaagtgttcc cttttgaata caactgtcat caaaagtact gttaagcaaa gaaaacgcga
     5101 ataaaaatta tcgccgagaa ccgtgagatc ggatttgaga aaaaacacat ccaaacagta
     5161 tattacaagc attataagat acattgaaat cagatccggt aatgctttgc ctttcggatt
     5221 gctcatcggc tcactccttt ttaagtattt ttcgctgtaa ttatatcatg gattgacaga
     5281 ctaatcaata caacaataaa aatttgattc ccatgcaggg acataaggat gatgagcaaa
     5341 ataaattctg taatcccgac atatcgaact gattttcagc ggcagagcga acaggtcgct
     5401 gttgcgatga taggcgcaaa cataaagctt agggcgaaag gtttttattg ttttcacggc
     5461 tccgtcaagt gccttctctt cgctgccctc tatgtccatt tttaacagag taatttcttc
     5521 tcccttgaga ataccgtcaa cgctgtttgc atcaacttca attttgccga acgatgaaag
     5581 ctttgaattt cttccctttt tcttttcaaa gaagagtgtt tcttttttat cccatgccgc
     5641 aagattaaat ctctcgattc ttgaaagctc ggatgttttg tcgaatagct ttttgaaatt
     5701 tttttcatcg gcctcaacgg cataaatttt ttgatatttg ttatcggtta cgttgagtaa
     5761 ttctcttatc gtgtcaccgt cgtatgcgcc gagatccatg ataatttcat tatcgcccgg
     5821 gcgaagataa tcggaatata tttcttcttt ttctctctcg catttaaaga gatattccgt
     5881 tttaccgctg actttaaaat tgagaacatc aatatagctt tgccgtgatt ttt
//
LOCUS       DAWBSW010000126         5868 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2556_length_5868_cov_2.358851, whole genome shotgun sequence.
ACCESSION   DAWBSW010000126 DAWBSW010000000
VERSION     DAWBSW010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2556_length_5868_cov_2.358851"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    752..761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1704 a         1190 c         1369 g         1595 t
ORIGIN      
        1 tccttcatgt tcaaccgctg cggcgtaatc acaatcgagg cagaggacgt tgatgaggag
       61 aagctcatgg aggatgcact cgaggcaggc gcaaccgatt tcatcaccga cgacgaggga
      121 atttttgaaa tccgaaccga gccgaacgat cttggcggcg ttcgtgacga ccttgaggca
      181 aagggatatt ccttcctttc cgcggaggtt gagtacgttc ccacaaccta caccaagctt
      241 accaatgagg acgacattaa caacatgaca aaaatgcttg atatgttcga tgataacgat
      301 gacgttcaga acgtatggca taactgggaa atggactaat acttgttgag ctggctcctt
      361 tggcgaacaa tccgtttttt cggctttgac gtataagcat acgcctacag ccgaaaaaaa
      421 cgaattcttc atccaaatta gcgcagctca attcttttgt atttttgtat aagcatacgc
      481 tgtcgctgag agaaaatgtc ttcttcaccc aaaatagctc gacccttact ttttgaagga
      541 actattgggc gcccctttag gggagcttgc agtggaaggg ataagaaaaa tgcattttta
      601 tatgatctga ctttttgagc tacctttccg ctggcggtgg cggggattta aagtttggtg
      661 ataaagagtg acagagaaaa agcggaggaa aaatgaaaag aaacgtttta aaagtcatac
      721 cttacggctt tataaaaggt attgtgataa cnnnnnnnnn ngtgataact gttcttcttg
      781 aactttatct ttcatcattt gcaagaaaca tatcagtcgg attggaattt ggactttccg
      841 ctttggcagc aattttgttt gctgctgttt attttattct tgaacgtaaa gaaaaaagct
      901 ttaaatcaat ttcacttttc tttctgctaa acctgctgtc atttgttttc ggcttagccg
      961 ctataactat actgattatt ttgcttccgc acagtttaat tgaattccgt gaaataaaca
     1021 atgcagacgg gttaatgctg atacttgatt tgagctgttt tatcggaata tcgctgattc
     1081 ttgaattggc tgtttcagca gtagtattat taaagaacaa agggacccca caatgaaata
     1141 tctttacata tggctgatca taatcaatat aataacattt gccgtattcg gaatcgacaa
     1201 gaaaaaggcg atagacggaa aattccgtat cagtgagtcg gcactgtttc ttttgtcgct
     1261 tctcggcggc tcactcggcg gatttattgc aatgcatacc tttcatcaca aaacccgaaa
     1321 atggtatttc aaattaggta ttccgcttat tctcgttgcg tggagcgcat tgattgcttg
     1381 gcttgttttt aaaaactcaa tatgattttc gcaccttgaa ggcttgtctt tcggggtgct
     1441 ttcctttaca aacacatctt tttgtctgcc gaaaagccga aaagctggaa ttatttttga
     1501 taatatgcta aaatgaatta tcttcatata tttcttggag gaatcgttat gtttaaaata
     1561 ctgattgcag aggacgatat tgagctttgc cgtcttttct ctcatgtgct gagtaaaaac
     1621 ggctactgcg ttaaaggtgt ttcaaacggt caggaggctc tcaatgagct tgataaggat
     1681 tattttgatc tcattatttc cgatattatg atgccggtta tggacggcta tgagctggtt
     1741 catcggcttc gtgaaagtga taatcacatt cctgttatga tgatcaccgc taaggatgct
     1801 ttcgacgata tgcgtcgggg atttatttcc ggcacggatg attatatgat aaagcccgtc
     1861 aatgttaacg aaatggtgct tcgtgtcggt gccttgcttc ggcgtgcgca aatgataaac
     1921 gagcgtcgtc aaacgatagg ctcgaccgtt atggatcttg attctctgac ggttacgacg
     1981 gagaatgaaa gctttatcct gccgcagaag gagttcatga ttctttacaa aatgtcagcc
     2041 tatcccggta agatctttac aagaaatcag cttatggatg agatctgggg atatgaaaat
     2101 gagtccgaca ctcacacggt cgatgtgcat atcggcagac tcagggacag attccgtgac
     2161 agtaaggatt tcaaaatcgt tactattcga ggcgtcggat acaaggtggt caagttatga
     2221 aaagcagaga aatttttgaa gaaatacgca gtcaaaagga tcagatattc gggctgagaa
     2281 tgagattgac tctttttgtt gccgctgagc tgatagtatg tattcttatt tcattcggag
     2341 cggaaatgct gttaaaaaac gtattgacct cggagtggcg cttgcctttg gcgattgcgg
     2401 gcttggtgct cggactgata atttgctttg gaataaccgg agcaatttca aactggtttt
     2461 ttgacccgat aaagcagttg agtaaggcaa tggaaagggt tgccgacggt gatttttcgg
     2521 ttcgcttgca gacgaaatcc cgtcttaaag agatccgcga ggtctattcg ggatttaaca
     2581 tgatgacgca tgagcttggt tcaattgaga taattcaggg cgattttgtt tcaaacgttt
     2641 cacacgaatt taaaacgccg atcaatgccg tagagggtta cgcaatgctt ttgcaggact
     2701 gcgataatct tgatgacgaa gaaaagaaat atgttgacgg tattattgtc agcactcagc
     2761 ggctttcgac gctgatagga agtattttgt tgctctcgaa gattgagaac cgctcgattc
     2821 ctacggatca gacagaattt tcactcgatg aacaaatccg ctgctcaatc gtcggaatgg
     2881 agaatatgtg ggagaggaag gacatcgaaa ttgatgccga ccttgaaagc ataaggtact
     2941 acggcaatga aaggcttatg attcatgtct gggacaatct tttaagcaat gcgataaagt
     3001 tcagcccgtc gggcgacaca ataataatca ggctcaaaaa cgaaatggag aaaatcgtat
     3061 ttactgtcga ggatcacggc gatggtattt ccgaggaggc gaagaagcac atttttgaaa
     3121 aattttatca agccgacagc tctcataaac aggagggcaa tgggctcgga cttgcgcttg
     3181 tcagccgaat tttgatgctt gaggacggcg aaatttttgc cgaaaatatt gaaggcggat
     3241 gtaggtttac ggttaagctg aaaagaaaaa gagcataggc acacgccttt ttcgggagac
     3301 ataattcgac aaatttcaac aaaagttgaa cttcagttga taatcagttg catttcacgg
     3361 tatataatca aaatgaaata aattataaac aaccgcggag tgataaaaat gtcgaaaaat
     3421 tatgttctct ttaatccgtt agcaggaaat aaaaggtgcg gcaccgatat aaaaatgatc
     3481 gaagatttga ttgagggcga aattgtgtat tgcaacatga caaaacccga aacatacagc
     3541 gatattcttc ccgagatgca gagcggcgat atactgatcg tttgcggcgg cgacggaacg
     3601 ctgaatcgtt ttataaattt gccgtacgat tattcggaaa agaacgaggt ttattatttc
     3661 cctatgggct cgggtaacga ttttgcccat gagctcggtc acaaatacgg tgacaagccg
     3721 ttcaatataa caaaatatct gaagaatttg ccagaggtca ctgtcaacgg caagacatat
     3781 cgcttcctca acggcatagg ctacggaatc gacggctatt gctgcgaggt cggcgataag
     3841 ctcagggaga caaccgaggg aaatataaat tacactgcaa ttgcgatcaa aggattgctt
     3901 ttccattata agccgactga ggcaaccgtt acggtggacg gagtaaagca taagtttaaa
     3961 agagtttggc ttgcaccgac gatgaacgga aaattctatg gcggcggaat gatgccgacg
     4021 ccgaatcagg acagaacagg cggaaagctt tctgtcatgg tttatcataa tctcggcaag
     4081 ctgaaggcgt taatggtgtt cccctcgata ttcaaaggag agcatgttaa gcacaaaaac
     4141 aatattgcta ttcttgaggg cagggagatt acggttgaat ttgatcgccc gacgcccttg
     4201 cagatagacg gcgagaccat actcggtatt tcctcctaca aaacaaaagc tcattatcac
     4261 gatatggtca atgcctaaaa aatacggact gtcataaatt cgacagtccg tgtttttatg
     4321 tttatttttt accttttagt atcggcgaga tcatcggata gatcgcaaag gttatcgcta
     4381 cgctgataag acccttgaca aatgtgaacg gaacgttgaa aaagagaatt gcctgccaga
     4441 gcgagccgac actcgggaga atctcgcggt atgcaccgag aattgcttct tcgggagcca
     4501 taagcttgaa ataaatcgga taaactaaga aataattgat aaaaaagctc gttgccgcca
     4561 ttgaaaaatc accggccatt gcacctataa aggcaaattt gcggttgtga tgatacttgt
     4621 agaacattcc tgcaacgaaa acgaaaacag cgccatgaat gaaattggaa agctctccga
     4681 ctccggcagt ctgggtcgca aacaggtgga taaggttttt gacaaggcag acggcaactc
     4741 cgcatacggg accgaaggca taagaggtga tgagtgcagg caggtcggaa atgtcaagct
     4801 tgataaacga cggtatgatg ggaattgaaa attcaataaa catcagcact gccgagagcg
     4861 ccgacatgat tgccgtgacc gttacctttc gcaagctttt aacttttgtg ttcataaaca
     4921 acaccctttc aaataaattc agtcagagtt gccaacgaac aaaaaatgtc ccgtacaaaa
     4981 actgtacggg actaaacttt tcaagaatac aaatcttctc tcatccggac tgtaccgtcg
     5041 gcttcggaat ctcaccgaat caactttcgc tcacgggctt gtggactgaa tcccattacc
     5101 gtcggtgggg aattgcgccc cgccccgaag acaactgtta acttatctct attttagcac
     5161 tgtatcacga aatgtcaata gtaaaattgt aatttataaa gcgttcacgt cgattgcagc
     5221 tattaccttt tcgatatacg ctccgaatct gtcggctatt tttgccgccg caattccaac
     5281 ctcctcgtcg gaaagcttac agtcgagcac acctgccgcc atatttgtca tgacggagaa
     5341 tgcaagcaga ggcatggaac agtgcgccgc cgtgagagcc tctgtaacgg tggacattcc
     5401 taccgcatcg gcgcccaaaa gccttgcggc tctgatctcc gccggagtct caaactgcgg
     5461 acccgggaag aacatataga ctccctcgcg gaaaatcaaa ccgcttccct cggcacactt
     5521 caatgcgatt ttgcgaagct tctttgtgta catatccgtc acatcaaaga aacggggacc
     5581 gaaaaagtca agattttttc ctctgagcgg acttgctccg ttgagcttta tatggtcgct
     5641 gactaccatt atatcaccga cattgtagct tttattgaca gcaccggcgg cattggtgag
     5701 tatggtcgcc ttaacgccga gaagtttgaa aaggcgaacc ggtataacaa gctgctcaaa
     5761 gtcgtagccc tcgtaggaat gaaaacggcc tgccatgcaa ataacttttt tgcagcctac
     5821 ctcgccgtaa atcagcttgc ctgcgtgaga agcgacggtc gaaacaag
//
LOCUS       DAWBSW010000127         5868 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2555_length_5868_cov_2.476862, whole genome shotgun sequence.
ACCESSION   DAWBSW010000127 DAWBSW010000000
VERSION     DAWBSW010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_5868_cov_2.476862"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2722..2731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3057..3066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4271..4280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2028 a          910 c         1383 g         1517 t
ORIGIN      
        1 gcggtgcagc tcgtgacaaa gtggtgcttc ccgattacag gtaagcatta caacggagag
       61 tataaaatta caagaccgga taccgataat ttgatcaagc ttcttaaaga cgttatgaca
      121 gaattaagct tctggaaaga cgatgctcag gttgcaagtg aaattacgga aaagttttat
      181 tccgatgtac cgggtctgtt tataagcata aaaggcgata tatgatgaac atttccgaaa
      241 tcattataca gctaaaaaaa catattgcaa tggaacggta tgttttttat cagagtagca
      301 gatacatacc ggcatgcctt gtatattggt ttgacagagt tcaaaatcaa tggaattatt
      361 ccgttgagct tatggatcca acgcataaga attcgacgat aagagtaaac gtcgagaaaa
      421 tcacatttaa acctgacgct gagaactacg aagaaatggg ggagtagata tgcaagtttt
      481 agttgcttgt gaggaaagtc aaagggtatg tatggctttc cgtgaaaaag gccacgaagc
      541 atattcgtgc gatataatcg actgttcggg cggacatccg gaatggcata taaaacaaga
      601 tgtaatcccg ctgctaaacg gtcattgtga gtttataact tgtgacggca aaacacatga
      661 aattgagaac agttgggatt taattatcgc acatccaccg tgtacatatt tgacgaatgc
      721 ggctacacgc agtttttcac ttaaagcgac gtcgcccgaa aaagttgtaa aacgttggga
      781 aaatagagca aaagcttcgg tgttcttcat gcattttgta ttggctgatt gtgacagaat
      841 tgtggttgaa aatcctgttg gatttatgtc aacggcgtat aaaaagcctg atcaaattat
      901 aaatccttat gagtttgctt caagcattga tgatagggaa aattatgtta caaagcgtac
      961 ttgcttgtgg cttaaagggt taccaaaact caaagcaaat aatttaccaa agcctacaat
     1021 ggaaaagtat ttttcaacgt atacaggcgg ttataagtcg aaagattggt gtacgtacgg
     1081 cagtcatagt tataaggatc gtcaaaaaga aaaatcgaaa acgtttccgg ggatagccaa
     1141 agctatggct gaacagtggg gataaggagg taacagcatg ggcttaaaac agcagccgaa
     1201 atgtaaagac aagacctgtc aattctgcga tgggaaatat tgtacgatat taaaatcaaa
     1261 gccaaaggtt tgtaattttt acaaagtgag ggttgaaaaa acggaggaca accgatgaaa
     1321 gaaatatgcg gaaattgtaa atacaacaga agaacatttg acggacattg caacgcagag
     1381 ttttgttgtg acaatgaaaa cagcgattat tacgcagttc cgacagctta tgatgatact
     1441 tgcgaggact gggaggaaaa agaatgtggc ttgtaaattt tgcaaatgac aattacgttg
     1501 aatttaatgc gattgacatt gaagatttaa tgcttttgat agttgagtat acaagcaacg
     1561 accgaaagta ttttgaaaaa gctatgagtg gttttaaaaa tgatttagac agagaaaatc
     1621 agtatatatc tctttttaac aatctctata atcgctatgg tgcagatgaa ataagttgca
     1681 tatatgaaat aaaatcaaaa ttcggggagt gaaataacgt gactgataaa aataatacaa
     1741 aaacggtaag ggtgaaagat aaaatcaaag caatttatcc tcatatagtg gttggaggta
     1801 aaattgataa gccgtgtttt tctatccatt ggtatgatgc taagaaaaaa gaaatgtata
     1861 tcggatattc gtcatataat ctcaattatg ttcgtgaatg gctaaaagaa gattttgaaa
     1921 ttgtcgagcc tgaaattgac agccttatca aacgtcagaa gacagagatt gaaagactaa
     1981 aatctatgaa tcaagcgaag cttgactgta ttcacgactt gcaaaaggaa aacgaaatcc
     2041 tttcaagaaa tgctgataca gcatttcaag acggtctaaa tgaaagacga gatttgtttg
     2101 cgcctgaaat caaagccgaa gcatacaaag aatttgcgga aataataaag gacaaatggt
     2161 tcgataatcg ttatgatagt ccaaatgtag actttgatga ttttattgac gatgcgctaa
     2221 aagaattggt aggtgataac aaatgacctg taaatattgt gaagacgaaa cctgcataaa
     2281 cgcagactgt cctgcttaca ggaattattg tccttgtcgg gactatgatt atgttgaatt
     2341 gtgtaagttt gctgaaaagg agatagatgt atttcaagga gggataaaca atggctgata
     2401 ataaaggttg cgaatattgc aaaggtaggg catactctaa aaagccttta actgtcatta
     2461 caagatacgg acgaagcatt aaattagcat ttgattattg cccgaaatgt ggcagacgga
     2521 ttaaaccttt tgatgaagat ttattagagg agcgtgattg acagtgacag ccaaggaata
     2581 tttgtcgcag gggaggtggc ttaatcagaa gatactgaac cgtaacgatg agattaggca
     2641 gcttcgggag ctggcggaga cgataaaggc tacaaactat gagcagagca ttgttcagac
     2701 ctcagggaag cccgatgccg tnnnnnnnnn naaaagatac ttcttgaaaa gcggtacata
     2761 agcttaaaac cctggactgt aatcgccgat gaaatgggct atgtcaggcg gcatatcacg
     2821 agaaaacacg cagaagcatt acagagcttt gaaaaaattc acacagaaat tttgaaaaat
     2881 gtccctaaat gtccttgaat gtccctcttg acatggtata ttattatact tggaaatata
     2941 gtaaagccgt gttgagaagt caatgcggct tttgcttttg gagaagctat gaataagaga
     3001 tgtaagaaat gcgtttggct tgagaggatc aacgctcaga cgacatattg tatgttnnnn
     3061 nnnnnntgtt taaacggtgc atttatggaa attcaagaga aaaagataaa agaactgata
     3121 ccttacgaaa agaacccgag aaagaacgat gcggcggttg agtatgtcac taattcaatt
     3181 aaagaattcg ggtttaaggt gccgatagtc attgacaaaa acggcgtgat cgtatgcggc
     3241 catacacggt ataaggcggc gcagcggctg aggctgaaaa ccgtgccgtg cattatcgcc
     3301 gatgatctca acgacgagca gataaaggcg ttcaggctgg cggataacaa ggtctcggaa
     3361 aaatccgaat gggatttcga tttgctttat gaggagcttg acggcatatt tgatattgat
     3421 atggaggagt ttgattttga cttgccggag gagcaggaga cgcccgacga gccgaaagaa
     3481 aaccaccgtg aggcaaccta caagaaatat aatcttgaat tggtggacgg cacaaggctt
     3541 gacggctttt atcagatgcc gattattcat aaggaagatt ttatccccga ggacttaatc
     3601 gggtttaatt atatgcttac cgcaaaggat aagaatgtcg gtattcattg ctttgttgac
     3661 gattatcaat ttgaaaggct ttggacttcg ccacaggaat atgttgagaa gatagccgaa
     3721 tacgattgct tcttatctcc cgatttcagt ctttatatgg acatgcctat ggcgatgaag
     3781 atatggaata tctatcgaag ccgactgata ggtcagtttt tgcaggactg cggagtatgt
     3841 gttattccga cgatatcatg ggcggagccc gaaacatttt ctttctgctt tgccggcata
     3901 gagaaaggct cggtagttgc ggtatcgact atcggagtga agcgcgaaga atcttctttt
     3961 cacgtctgga aaacaggtat ggacgaaatg atacgtgtgc ttgagccggc tgctattctc
     4021 gtttacggcg gcgaggtcga atacgattac atgggaataa aaggctgttt attatggtaa
     4081 tcatgtgatg gataaaatga aaaacttgaa aaataagggt taaaggatta taatgggtgg
     4141 cagaggtcaa aactattcaa ttcaaaagag gttgaataat cataaaaatg cagtcattgc
     4201 aagaaataaa ctgaaaaact atattttaaa tcctacaaaa gatgagagta aggcaaaatt
     4261 ctttaaatct nnnnnnnnnn tctcttggat ataatatgca aaattacgag cgtctcgaac
     4321 gggatatgag agcaaaatta ggaaccagca aagccttgaa atatcaaaca aatcccgacg
     4381 aaagtgttag ttttcaggtt aatatgtata taggaataga taaaaaagct cttgtaacaa
     4441 ctgcatggag aattgataag ggtagcaatg tgccgagatt cattacagca taccctaaca
     4501 gatcaaaacg tgttggaagg tgattaaatg aaaaactttg aattgtttga taaagtaaga
     4561 attaaaagca aaaatgtgat tggttttatc gtcgatatcc atgaaaatgg gaaattggat
     4621 attgaaaaag agggaaatca aggacccgtt tattggtata ttgatgaatc agatatagag
     4681 aagatagaat aatttcgggt tgactttatt atattaagaa acaaaaccgt tggctgtttt
     4741 acagtcagcg gtatttttat acccaaaaac taaaggaagc gattatatga taaaaataaa
     4801 tatacagctt ttcggcggca gaggagcaag ctcgggagct aatacttcaa ataagccgtc
     4861 agctcttgag gtatttaaag aaagcgcaaa tcaattcaaa agagctttac aaaacggcaa
     4921 ggagaacaaa gcggctattg tcgagtatac ggatgtgaca ggaaagactt ttaaaagata
     4981 ctgggacggt tctacataca gagaccgacc cagttcaatg tacaactctt taagcaataa
     5041 agatatgtcg ggtactttta aatcaaaatt tgaagaaccg aaagattgga gaaaacgctc
     5101 aaagagtaaa taaggtggta atgtggcgaa tgaagaaaac ttaaaaccgt ttacaagtaa
     5161 tcgaagcagt gaggaagcag tgagaaacgg caaaaaaggc ggcgttgcga gcggaagggc
     5221 acggcgcagg aaaaagctgt taaaagatac ggtcaatatg cttttggctt tgccgatgca
     5281 ggagggacag ctcgacaggc tgacggattt aaaatcaatt aaggataaaa atatcacggt
     5341 tgaggaagct atggtattaa agcagatgca aaacgctatg aagggcgatc tgaaagcttt
     5401 tgaaagcata atcgcactgt ccgaatctca taacaagaca ggcgagcagc cgaaagcaca
     5461 ggagaccgac aacgcattta ttgaagcact gaacggcaag gctgcggagg cgtggaacga
     5521 tgcagaaaag gaataaattc aaatgggagc cgttcagcaa taagcagtta aaggttatga
     5581 cttggtggtg cgataattca cccgtcaagg actatgacgg cattatagcc gacggagcgg
     5641 tcagatcggg taaaaccgta tcaatggcta tttcgtttgt gaattgggct atggcacgat
     5701 ttaatgaaac ggattttgcc ttatgcggaa agacggtcgg aagcttgcgc cgtaacgttg
     5761 ttattacgct caaacaacag cttctgagcc ttaactatga gtacgaggaa aaacgcacgg
     5821 ataatctcat aatcatttca aaggggaatg taacaaatta tttctatg
//
LOCUS       DAWBSW010000128         5810 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2585_length_5810_cov_2.574978, whole genome shotgun sequence.
ACCESSION   DAWBSW010000128 DAWBSW010000000
VERSION     DAWBSW010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_5810_cov_2.574978"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2824..2833
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1642 a         1285 c         1126 g         1747 t
ORIGIN      
        1 tatagtgata cataataaca atctcctctt cgcccatgcc acccattcag cgtttctcgc
       61 taaaccatta tagtatactc acgaatgatg cccgtgtcaa tactttcagg taaaaaagaa
      121 gcggatgtaa ctgaaatcag ttacatccgc tcgatatggt tgcggaggct ggacttgaac
      181 caacgacctt cgggtttgtc tcacaaggcc ttgccttgct tgcccctcga aaacatagtc
      241 gctctgctcg cttggagcgc ttcacagttc tccttgtttt ctcacctcag caccacgcct
      301 gtatccgcca ccggcggcgg cgtgccgctt cgccccaacg agcgtagctc gtcgcgtcat
      361 aaccctgaaa gaaaaaaaga gcaggtctct tgacctactc cttcatggtt gcggaggctg
      421 gacttgaacc aacgaccttc gggttatgag cccgacgagc taccaactgc tccactccgc
      481 gatatttctt tgcccctctc tcaaggcgct taattattat agtactgttc atctgttttg
      541 tcaatagttt tttattattt ttttcgtttg atatgatttt attcatatat gaatatttat
      601 attcttaaat attgactttt aactcatact tttatataat ataaaaagtc tatcatttta
      661 ataggttaat tcttttaaaa aggcggtgac ggtatgaatg atttggttga taagctcttt
      721 aacacaggtg atttatctga tgaggagtta aaaacattgc ttgaaagcga cagctttaat
      781 gagccgctcg cccatgctgc agatatccgc cgcagagaga attacggcga taaggtttat
      841 atccgaggtc ttatcgaatt caccaactat tgccgcaaca actgctacta ttgcggtatc
      901 agacgtgata ataaaaaagc cgaacgctat cggctgacaa aagaagaaat tcttctctgc
      961 tgcgacgagg gctatcggct cggcttccgc acctttgtta tgcagggagg agaggatccg
     1021 tactatacgg acgaaatgat ttgcgatatt atctccgaga taaagcgctg ctatcccgat
     1081 tgtgcagtca cgctttcaat cggagaaaaa tcgagaaaaa gctatcaggc atttttcgac
     1141 gcaggcgcag acagatatct gcttcgccat gaaaccgcag atgacgagca ttacggcaag
     1201 cttcaccccg gctcaatgag tcttgaaagc cgaaagcgct gtctttttga tttaaaggaa
     1261 atcggctatc aggtcggttc gggtttcatg gtcggttcgc cgtatcaaac gatttcaaat
     1321 ctcatttgtg atttgcgctt tttgcagaag cttaagcccg acatgattgg tataggcccg
     1381 tatattaccc atgcggacac gccgtttgcc gagtttaaaa gcggaaacgt tcttctcacg
     1441 ctaagacttg tttcaattct caggcttatg ttcccctatg cactcattcc gtcaacgaca
     1501 gcactcggaa caattcatcc gcagggtcgt gagcttgggc tgaaggcagg cgcaaatgtc
     1561 gttatgccga acctttcccc tgtttcggta cgaaaattat attcactcta tgagaataaa
     1621 atttgcacgg gggaggaagc ggctcagtgc cgaggctgtc tcgaaagacg tgttgagtcg
     1681 gcaggatata aaattgtaac ggacatcgga aatgtcaaaa agatcgagga ataattatgc
     1741 ttaatcagtt ttcaagaaca gagcttatat acggacacga ggcaatggaa aagctgaaga
     1801 attcccgtgt tgcgattttc ggaatcggcg gagtaggcag cttcacagcc gaggcacttt
     1861 cacgctcggg agtcggcact cttgatttaa tcgacgacga caaggtttgc ctgacaaaca
     1921 tcaaccgcca gcttcttgcg acacacaaaa ccgtaggcaa gtacaaagtt gacgttgccg
     1981 aagaaagaat tgccgagata aatcccgaag caatcgtgaa caagcacaga attttctaca
     2041 caccggagac tgccgatcaa ttcgactttt cacagtatga ctatatcgtt gacgccattg
     2101 acacggtcat aggcaagctt gcgcttgttg aaaacgctaa caagacaaag acgccgataa
     2161 tttcctcaat gggagcaggc aacaaggttg acccgacggc atttgaggtc gcggatattt
     2221 ttaaaacctc tgtctgcccc ttggcaaagg taatgcgaac cgaactgaag aaaagaaaaa
     2281 tcaaaaagct caaggtggtt tattcaaagg aactgccgat aacgccgatc gacgatatgg
     2341 caataagctg ccgtcagcat tgcatctgcc ctccgggaac agcgagaaaa tgcacacagc
     2401 gcagacaggt tcccggaagc actgctttcg ttccgtcggt agtgggattg ataatcgcag
     2461 gcgaggtcat aaaagacatt acaggcttca aaggcagagg aataggagca taacaaaatc
     2521 atggcagaat tcagaacaga gcgcaagggt gatattatta aatgcgaata caccgtcagc
     2581 ggcggaattg agggcggggc ttactctctc agggcgtcca aaacaggcga taaaacggct
     2641 gagctcgccg tgtcaaatgt tgttaatcaa atgacctttg accgtgagaa atattatgac
     2701 atcggcattg aaatttttga tgagcttgca agaatcgttg accgatacag aatgctgcga
     2761 tggtctgcca ggacggaaaa tcccgtactt gtcagtgatg aggaaataac gaccgtgcga
     2821 attnnnnnnn nnntccgagg acggcgaagc aaaggcgtat aaaatttcga gcaaacagat
     2881 tttgcctttc aacgcaatga aggcgttcaa ggaaattcgc aaatgtatgg aacgctatat
     2941 tgaagaataa aaatcaccga caggatttca atttgattcc tgtcggtgtc tttttatgtt
     3001 atagacataa aaatttcatc aattacttca tcgagaattt tgttgtaaaa tttctgattg
     3061 ctcttcatct cagaaaagct tgaataaaag cttttatttc cgttgtgaga aacacagatt
     3121 ttttcgtctg tttttttcaa aatatatctt tcattgttta atgaaaaagt tacggtcttt
     3181 ccctcattca atcccgtttc aaccatcgtg acgttgttcg gaataagtgg catatcataa
     3241 taattcttaa aatcctcata acactgtggg caaacccata acccctcatc aatagttgaa
     3301 tatccctcct gaatggagcc cgaactcaat ttttcttcgg caaatttttc tgtacaataa
     3361 atgcaatggt catgatccca tcccggacgg taagcatgat aaacattttt cttgaaactt
     3421 cgaccatgaa tataagaatc ctgtgacatt attctccaat catttttttg aagcatagcc
     3481 gatacctcca aataagtcaa atatttttcg ttcccaggaa ttatcttcat aaattctata
     3541 tccgttttcc cacccattgg gattaatgta atcccaatga ggtttcatcg gtccggcatg
     3601 atttaaatcc caatggtaac tttctttagt ttttggatta taccaattac ctttattatc
     3661 ttccggagac gagctgcccc tccattcaaa accctttccg ggtgatttgt ttttgtatcc
     3721 cggaaatttc ggcatatctc tgttctcatc aaaagtcggc ttcgggttgc ctgctccgat
     3781 tccgcctgct gatccgccgc ccttttattt gtcacttaca aggtaaacat cgaaatcttt
     3841 tcttttacct cctacgacac aagtttcaac ctcactgtcc caaaaaggta aaagatcatt
     3901 ttcgtaataa tcttcaatca aagccttttc tttcaaattc tgagataaaa attcatcata
     3961 atttttaatt ataaaaacat atttatcttc tgcaagttcc aaaatatctt ttaaaaaatc
     4021 attttcggtg taaaaatcct ttttccaacc atgaggaatt tcgccgaagt taaaacagtt
     4081 ccacaagcgg tcaaaatatt cttcatatgt attaactccg tcaccgtcca taacaatata
     4141 ttttgtatca tcacgggcat aaagaatttt tcttttttca aattcttctg ttgtcattct
     4201 gtataactta ttcatatgca tcactcctgt tctacttcaa taaaagtttc ataatgatcc
     4261 ggtgaataat aaacatcacc atcattgtct cttactaatc gttcattatc tcttgatccg
     4321 ccaaaaggtc gactgtttac atcatattca cgatagtcaa gtctttgatg tcttgagtta
     4381 tagcttggta gctttccttc tctattgcta tattttcccc cggcgcttgt gccaaccgtt
     4441 tgccctttat agtttccttt ccaccctact ttttcatatt cgatatatgc tctttttaca
     4501 ttttcgggca aatcgtcaag cggaactttc gggatattgc cgcccggctt cgggttgcct
     4561 gctccgattc cgcctgctga accgccgccc ttttcgcgag gcaaaacata tccggtaact
     4621 tttgtcacgt atataggttc atagtgtttg agattgtttg tttcggcata atctatttcg
     4681 attatatcgc ctttcatatc ctcaaaaggc ttgccgtaac aaattatttt cttcggtcgt
     4741 atccgcctgc gcatttcatt atagccaagc ataaaatgag acttttcatt ttttattcca
     4801 agcgttgaaa cggcaaccgt acagccttgt tcaattccgt caaagcagaa attgaagctt
     4861 tcaaggctgc cccaccgtac ggtcggtatg accttaatac cctttgactg cagatacgcc
     4921 ccgacccacc tgtttctgaa gcagttatac aactgcaagc tgacaggcat ttcggtaaac
     4981 atgctgaagt caggcgacat aacggcattg aattttttta gccgttcaat cttgtcattg
     5041 ggatttttat aaatgctttc aaaacggtaa tcatcaagaa aaaagtgtac cgttttttct
     5101 ttttcgtcgc tttgattgat tttatcataa cctataagcg acagtttttc aagagaaacg
     5161 tcctgcattt ttatcagagg cattgaaaac attccgacgc ccttaaactg atttctgaga
     5221 aaaagaggat catttctgaa ctcgaagctg ttaattttta tcattccttt caagtattat
     5281 tgagaaacaa tcttctcata aatagcttaa aaagaatgct ttcataacct tatagggtta
     5341 tatttttcag atttcaccaa tttgtttgca aaacggatac atttatacct gttaaaaact
     5401 gtaccaacat cggcaaaaca catcgcgccg agaaaaacgc ctttgtcgaa cctcaccgta
     5461 ccatattttt cttgtcaatt tttctgttgc catataacta ccgtccctgc ataaacatcg
     5521 gcaaagaata tcagcatcaa gcattttttt gactttaata aatatttgta tagaattatc
     5581 tgaaaaatct tgacaaattt tttggatatg atattataaa tatacatcca ttggggagta
     5641 gctatgggat ttttcctatc tgctgtcgcc acgcgcttta aaggcttgaa atatcaagtc
     5701 attttcagcc ggcagcagac cgatgcgatt tttcgtgttt gatgcaagac ctttggcaga
     5761 ctataaggct ggcagcagac cgatgagatt tttcgtattt gatgcaagac
//
LOCUS       DAWBSW010000129         5781 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2594_length_5781_cov_2.152462, whole genome shotgun sequence.
ACCESSION   DAWBSW010000129 DAWBSW010000000
VERSION     DAWBSW010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_5781_cov_2.152462"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    791..800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1541 a         1219 c         1331 g         1680 t
ORIGIN      
        1 ttgtttccgc cgtggcgctt gcggttacaa gaccgtttgt aaaaaaattt accaaagggc
       61 gaaaacagcc gacaaatgcc gaccgctata taggtcagga ggcgacagtt accgaaaaaa
      121 tctcaaatga gctttcaacg ggagctgtca gaatcggagg ccttgtatgg acggcgagaa
      181 cggtggataa ctccgaggcc gaggaaggcg aaagagttac tgttgaagca atcgagggag
      241 ctaagctttt agttaaaagg agagatgatc tatgagtccc gtaggaatcg gtattgtggt
      301 atttttaatt cttttggcag ttcttcttat agttacaaac gttaagatcg tgccgcagtc
      361 aagagcatat gttgttgaac gtctcggcgc atatcgcgag acatggcaga cgggtattca
      421 tcttaaaatc ccgttctttg agagaattgc aagaaatgtt tctcttaaag agcaggttgt
      481 tgattttccg cctcagcccg ttatcacaaa ggataacgtt accatgcaga tcgacacggt
      541 cgttttctat cagattacag acccgaagct ctatgcatac ggcgttgaaa gcccgatttc
      601 ggcaatcgaa aatctttcgg caacaacgct tcgtaacatc atcggtgagc ttgagcttga
      661 ccatactctt acttcaagag acactataaa cgctaaaatc agaggaattc ttgatgaagc
      721 gaccgacccg tggggaatca aggtcaaccg cgttgaattg aagaatattc ttccgccgcc
      781 ggagatccag nnnnnnnnnn taaggtgctt gttgccgagg gtcagaagga gtctcagatc
      841 cttgccgccg agggcgacaa gagagccgca attctccacg ccgaagccgt taaggaagcg
      901 aagattcgtg aggcagaggg tgaagcagag gcaattctca aaattcagga ggccgaggca
      961 aaggcgattg agcttatcaa tgcttcaaac ccgggcgacg gctatattaa gataaagagc
     1021 cttgaagcgt tttcaaaggc ggcagacggt cagtcaacaa agatcattat tccgtcggaa
     1081 attcagggaa ttgcaggtct cgcagagggc gttgtcgaag cggtcaggaa ataaaaataa
     1141 ataaaaaata caggctgtat tgatcaagtt gaccgataca gcctgtttcg tttttaatcg
     1201 cagttattta ataaaaatcg aaaaaataat ttgcgcagag atttgaacga gctttgtaag
     1261 aaaagcgaat atctgtgaaa ttgcggtgct cttgagaagc tcatcggtaa caggggagag
     1321 tgtgtttgac ttgctgttat atttcataaa ctgggggtag ctttcgctgt cgtgaattgt
     1381 gagctgcttc tcgctcttgg caagccaaat aaggaaatcc gttgagttgc cgacgttgta
     1441 atcgacatga gccatgtcgc gaatgaacca tgtctgttcg gggaacatac aggtcgatgc
     1501 atcaagctga cggtcagcgc tgaggtggtt atgtccgtct gctttagctt gaatgtagtc
     1561 ttcgggaagc gttttgtcaa gctttgagca aatcgcaccg cccgaagcat aatttacatc
     1621 aataagaaaa tcgttattgc ttgttgttga gctttcggaa atcggaaggc cttgcatatt
     1681 gtactgcgcc gtaatataaa tcgttgtgtt tttttcagcg gctttcagaa tgtcgtaagc
     1741 ccttgcctga acgttgcggt gatactcatc aatcgctttt ataagttccg tatctgctcc
     1801 ggcaagcatg aaatttttag actcttcata atttcttgca tcaaccaatg cccaaagtcc
     1861 gggcatatat ccgaaaacgg ggattataag ctccttataa agtctatcat tgagatttgt
     1921 gaggatattg tttatgtatc cgtcaatcga agcgttgatt ttatatgcat caaggctgtt
     1981 gttgaggaac ataataagct cgtcgagaaa atcgtttctt gtaagctgag ccattctgcg
     2041 aatgaggccg taagcgtcaa tgctcatgtc acccgagaac atcgagccca tgcacgaagt
     2101 gccctgaaaa gcggtcgagc agagcgaaac actgtcaacg tctttgctgc cgtatttata
     2161 gagataggag agcgtgactg ttcctcccat gctgaaagcg gcgagtgcta cacggtcaca
     2221 cttggtctca gccttgacat tctttataaa tttattaagc tcgtcggcat gcttgagagg
     2281 gtcaagtctc cagtcgtaat tgaagaaata tgtgttgtct gcgccgaagg cttcgattcc
     2341 cgccttgaca acgccgccct cgtccttgac ctcgttcatg aaggagtcgg ggtagttgcc
     2401 tgcgctcata ggaaacagat cggtggtaat ttcatatttt gagctgccgt ccgggttgca
     2461 ggcaaggtcg tcaaaggctt cctccgccgc agggaaaagc gcgtcgccaa gcttgtcgta
     2521 atcgccgtcc ctgaagaatt cgacaagggg aagaacaatt tttgaggcaa gcttcatgat
     2581 tgtttttgtc gatgtcggga acaccttttc actgtcttta tatagaggaa aagtgttcat
     2641 tccgctgaca acgataaacg ctgtcttagg ctcggttgca aatgcgggta tcagtgagca
     2701 aaaggcaacc gttaggcaga ggataacgct gataagaatt ttagctattt ttttcatatt
     2761 ctttatcctt tcaaaaaaga ggtgttgcat ttatattcaa aaacgcaata cctccggaga
     2821 attatttctg atatgtgtta agaaatgctt tcgttctctc gtttttcgga ttgacaagaa
     2881 cctcgtcggg tgtgcccatc tcggcgataa cgccgttgtc cataaagatg acctcgtcgg
     2941 aaacgtcctt tgcaaagttc atttcatggg tgacgataac cattgtcatc ttgtcctcgg
     3001 cgagctggcg aatcgtcttg agaatttcac ccgtaagctc gggatcaagc gccgaagtcg
     3061 gttcgtcgaa gaagagaatt ttcgggttca ttgcaagcgc acgggcaatc gaaactcgct
     3121 gctgctgtcc gcccgagagc tggcaggggt agaagtccag cttgtctgaa agaccgacct
     3181 tattgagaaa atattttgca gtcttatcaa tctcctcaag catttccttt ttcttctgct
     3241 tatagtcggg acgccgtttt gccgcaagct tcattgcgag cgtaacgttg cccagcgccg
     3301 tgtgattttg ccggaggttg cggtttcgag cccgttgata cagcgaagaa gcgtagtttt
     3361 gccgcttccc gaggagccga tgacggagag aaccttgccc tcctgcaatt caaaagtaat
     3421 gcccttgaga acctcggttg cgccgaaatt ttttgttaag tctttaactt caagtattgc
     3481 catttctcag ccctccttaa actttgtaga agttgagccg cttttctatc ttgttgaaaa
     3541 ggattgtcag cagtccgctt gcgacaagat agaatacacc tgaatagaga agcggccaga
     3601 gaattccgct gcctctgatg tagctttcgg caacaaatat aatctcataa tacgtaatta
     3661 ttcttgcaag tgatgtatct ttgacaagtg taattacttc gttgctgatt gggggaacaa
     3721 catttttaat gacctgcaaa agaattactc ggaaaaatgt ttctgttttt gtcattccga
     3781 gaaccttaca ggcttcgtac tgtccggtgg ggatagattc gatgccgccg cggaaaatct
     3841 cggaaaaata gcaggcatag ttaaaaataa aggtaagata aaccgccggt tcgcaagcca
     3901 gcatgaattc acctttggtt agaagtccgg gtccataata aaaaacgata agctggagca
     3961 taagcggagt gcctcggact atccaaacaa tggttcttac gagccagcga agcggctgaa
     4021 atttgcttct tgagccgaag ctgataatca atccgagcgg cagggcgaag gcgagcgtta
     4081 tcgcaaagat ttttagggtc ggcaaaaagc cgttgacgaa caggttaacc gttacgtccc
     4141 aaaatgatgt gctcatgtgt taaatcctct ctgaaacagc agtatagggt aaaggtattt
     4201 gcccttaccc taaaactgta tttaagtgcg ttattttctt aaattatttt gtggcctgaa
     4261 aatcagttac aatgctgtcc tgaagtccgt atttttcggc aattgtctta actgttccgt
     4321 cagcaacaag ctctgaaatt gcagtgttga cagcggcaac gatgtctgaa ccctttctga
     4381 atgcgatacc gtaggattcg tcatcgccga gcttaacgtc cttcatgatt gaaaggttgc
     4441 tgtatgtctt accctcaaca agcattgagc ctgccataac gtagtcgata acacatgctg
     4501 cagcctgtcc gctctgtacc tggagaagag catcactctg gctttcaaca ggcttatagc
     4561 tcttggagag tgttgcattg gcctggattg cggattcacc tgctgagccc tgctctgcaa
     4621 ctatggtctg acctgcaaga ctttcaacgg tagtgtactt agcgtcctta acaacaacta
     4681 cctgatagtt tgtcatataa gcgcccgaaa catcggcaac attcttgatc gcatcggtga
     4741 ttgtcatacc gttccagatg gcgtcgattt tcttagtgtt aagctcattt tctttctgac
     4801 cccaggtgat taccttgaat tctgcagtaa cgccgagctt ctcgcatact tttctcgcaa
     4861 ggtcagcgtc aaagccgatc cattcgccgt ttgcgtcttt gtagtccatc gggtcgaaat
     4921 cggtgatgcc gataacaagc tttccgttgt ttttaacgta ttcaaggtcg ctgtccgtcg
     4981 cagttttctt atcgccgcag cctgcgaaga tgaatgtcat tgctgccatg agtacggcaa
     5041 gcgtgattgc aataattttt ttcataatgt tttcctccaa actttattgt ggtagtatag
     5101 tagcacatta gcgcactaaa gtcaatagct tttttaaaat ttttttaaaa aaatatttaa
     5161 ccgccgattt aattaaaaaa gggcggtttt agagttgatt ttttagggtg taaacggcag
     5221 gattattatt taatcttata tttctctttg tattcggcaa ccatttgcgc ctcttttttt
     5281 tcggctttta ctgttttaat atacctgtat acaaagaacc ctgccagaag aaaccagcaa
     5341 acggtgagca tataccaagc tccgtttgaa taaattttgt gctcgacgat caaaagaacg
     5401 attgaaaatg ctccggcaag cacgccgaga gctgcaaaaa caatgtaaag caccttttta
     5461 ttgattatat ccgaaagctt ttccttctcc atatcagcac ctccgtgtgt gtttagtaat
     5521 ctctgcttta attctaacat atataaattt attttgcaat atttaatgca tcacatattt
     5581 tgatataagt ataaacatta tcattatcgt aattataact aatgccgcta taattttttt
     5641 ctttgagcct tttttgtttt catttttcgt ggatatatga atatcagcat gatcaacatt
     5701 gaatgttttt tcgttataat aattcctgta ctcgatatcc tgtacaattt ttttaaacga
     5761 tgaactctta ttgtactttc c
//
LOCUS       DAWBSW010000130         5773 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2600_length_5773_cov_2.590416, whole genome shotgun sequence.
ACCESSION   DAWBSW010000130 DAWBSW010000000
VERSION     DAWBSW010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2600_length_5773_cov_2.590416"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3705..3714
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1470 a         1282 c         1292 g         1719 t
ORIGIN      
        1 gtgcaaggga aaaaccattg ttttttttgt catttaacat agttgaaaag ctcctgatat
       61 ttatttctat ccaaatcctt cttgtaatca gtcatcattg ttctgaaatc tgcatatttt
      121 gggatgaagc cgaaatcata ctttgccttt tccatagaga acaaataaga cggcgtattg
      181 tttacaattt ccggtttgta ggttattttt gatttttgat tctcggacgg tgcccaaagg
      241 tcacggataa cctctgcttg ttcttgaagc gtaacacctc tgcctgaggt catgttataa
      301 agtccgtatg tctgctcgct tttcattgca agataaaagg catgagcaac gtctttgaca
      361 tagataacat cacggctgag attagggtca ccgaaaatgc agatatcctt gccctcactt
      421 gcattgtcaa tgaaaatttt aagtcctgat tttcttagag tgccgttaat ataaaggctg
      481 tcatgcggac ctacaccgta aacgggagga aaacggaacc atgcgttttt cattccgtgc
      541 tgctgattgt aatattcaag cacgtcgttt gccgcatttt tggagaaaac ataaacggcg
      601 tggtcgcctg tgtatatgaa atctctcggt tcgtcctctg taattacgcc tttgccccat
      661 gcacctctta catcggcata ggagcagttg gaaataactc tttttatccc gtttttgcgg
      721 caatattcga gaacgttgat cgttccgatt gtgttaattt caaaataatc cgccgcattt
      781 tcgtctgtat taaggtcaac ctgtgcattg gcgggcagta agcctgcaag gagaataacg
      841 ccgtcaactc cctcttttgg aagcttgtca aaatcctttt tctcggtaag atcaaggttt
      901 atgtatggaa cgccaaggcg gtcatagtgt tcacgaaatt tgttgtttct gcctgtgacg
      961 gtgacttcac agccttgctt tataaattca tcgacagtat atacgccgat gaagcttgat
     1021 gcgccgataa cgatatacac ggcaaaaccc ccgttgtaat aaaattaaat aactttatat
     1081 gtattccttg attacatctg tataattatc gattatgtag tcaacctctt cgtccgtcag
     1141 acggctgaaa agaggaaggc tgatttcatt tgcgtaatgc gcgtaggcat tgggataatc
     1201 cttgatgtca aagccgagct ttttgtatcc ggtcatcatg ggcaacggct tataatgcac
     1261 atttgtcgta attcctcttt ctgccagctt tataattatt tcacggcgtt ctccgtcgct
     1321 tacaggctct ccgttctttt tgaaaattct tgtaagatag agatgtcccg aggaaatatg
     1381 ttcgtccgtg aagtggggga gcgtgtgaac tccgaacggc ttaaaggccc gatcgtattt
     1441 ctctataatc tctcttcgac gcttcaaaag tgcagggtag cgctccatct gagcaagacc
     1501 gatagctgca tggatatcgg tcatattgca tttgtaccac ggaccgagaa tatcgtattc
     1561 ccatgcaccg agctggtttt tgctcagtgc gtcctttgac tgaccgtgaa gcgaataaag
     1621 ctgaacctga ttatatatct cctcgtcgtt gaaatcctca cggggcttcc aggtcagtgc
     1681 gccgccttcc gccgttgtga aattcttgac ggcatggaac gaaaatgcgg aaaaatcggc
     1741 aaccgagccg ataggcttac cgtgccatgt tgagccgaac gcatgagcgg tgtcggcgca
     1801 gacgattatt ctgccgaact tagactgaat atcattggac ggagtgaaaa gctccttttt
     1861 cttctcaacg attgagaaga ttcggtcata atcgcaggga acacctccga tatcaacagg
     1921 gataatagcc ttggttttat ctgttattgc tttttcaact gcgtcataat ccatttcgag
     1981 agagtcgggc tgactgtcga taaagatcaa ccttgcacca acatgacaaa caatctctgc
     2041 cgaagccgtg taggtgtatg cgggaacaat aacctcgtca ccctcaccga tgccgagcag
     2101 acggagcgcc atttcggcac atgaggtctg agatgagaga caaaccgctt tgttggtgtg
     2161 acagagagcg gcaacctccc tttcaagctc cttggtttta ggtcccgttg tgatccagcc
     2221 cgattttata gctgaggcaa cttcctcaat ttccgcatcg gtcagatccg gcggagaaaa
     2281 cggtattttc ataaaaacat ccccttcaga aaattcctat ttctttttta aatcataatc
     2341 tgtaatcagt ttaatacgtt ctttcgcctt ttttacggcg tcccgttcaa gctcggggtc
     2401 acgcttgtga cctacatact tgactattct cttgcaatat aatgatggca gtttccatgg
     2461 cttatcataa agctcggggt gcattgccga cattaaatcg taagagggga agaccgagcg
     2521 cagaagtccg aatctgcttc cgccggtttt tgaaaaaata aacgtacttg cgtaggttat
     2581 ctccttatca tcgagtccga aggcacctcc gttgataaga tcctcacaaa gtgtgctcgg
     2641 atcgagctgc tttgacgggg tgttttcttc gccgccgaga agcttcataa gctcaacggt
     2701 gtcggcataa aaagcacctc cgataaattc ggaaaaaacc ttttctgcag atgaaaagtc
     2761 gatttctttt ttatatttat ttccaaacac ggcgagatcc atgatgtgtc tgatgcctgc
     2821 gccggtggtc ataaaatgct ttagataatg taaaaataaa aatatataat gatttgtcgg
     2881 gtcaagcgtg tacagcctgt tgccgtcata attgatttcg taaggatttt tcagcgcttt
     2941 gtcaaaaacg cagttcattg ccgtgcggaa cgaattctga gtcccgaaaa gattcaggtg
     3001 aacctcgatt ttcaaatgat tgcgtctgtt atgaaatgaa acggtctgta tcctttttaa
     3061 atattttaag gaaaagtcac cttcgttgtc tgccgtatat ccgttttcgt tgagaacacg
     3121 gaggcagagt gaaaagtcat gctcggaaac atatatatcc tcatcgccgg aagcacggtg
     3181 attttcaagt ctgccgtaag ccttgcggca gataagaccc tttaaaacta tgggtttcag
     3241 tcctgccgag agaagctttt tatatattga ataaaactca gccgtatttt gcgtttgtgt
     3301 gacaaccgag cttgtcgatt ttgatatcag ctccgcttta acggcaacag gggcagggga
     3361 aaactccgca tatttaactg caccctcaag aaagatcggt gcaacctctt gctgaaaagc
     3421 caagcctgtc agcagcttga aatcgggctt gtccaattca aagacctcgc ccgttaaggc
     3481 tgtattcagg attttgaaaa gattgtcata tcggttattc ataatgagta tctttcctgt
     3541 ccaaaagcac aatgccgttt tcggacacgg aataaatgct gtccgaaaga ttaaaaacgc
     3601 tcggcttgtg cgatgtgaga attatcatct tgttgcgatt gtatgtgagt atatttgata
     3661 taacctcttc ttccgtttca acgtcaagtg cgcttgttgc ttcgnnnnnn nnnngatttt
     3721 gtccgtcaga gaagcattgt ccccgttcgg tgatttttgt atttatcgtt tcggggagct
     3781 tgctgacaaa ctcccatgca caggcgtctc ggagaacctt tataagctcc tcatcggttg
     3841 cgtccggctt tacaaggcgg agattttcgg caattgttcc cgaaaaaacg gtgttgtttt
     3901 gcggcacata gctgaaaagc tttcttgttg ctgcgcttag ctcaatcctg tctccgtcct
     3961 cgttcgttac ggaaatactg ccggatttcg gcttcataag gccgagaaaa agcctgagcg
     4021 ttgaggtctt gcccttgccc gaagcaccga taagtgcagt gattttgccg ctctgtgcgg
     4081 tgagcgagga atttttatat acaacagcgt cgtcattata tgcacaggtt atatccttgc
     4141 atacaagagt cagcttcttg tttttacttt tatctataaa ttgtttttca tattccgtta
     4201 gttcagtttt ttcggtcgga agctcaagca catcaagaac tctgcctgcg cttatgcctg
     4261 ccctgataaa ggagggaacg agagaaacga cagagctgaa cgagccggtc agtgaagctg
     4321 ccattgacat gaaaagcgaa agagttccgt atgttatgtc acccttccaa agtctgtaca
     4381 gtgcaaaacc gtaacaggaa taggtcacaa gctgacccat aaggctcata actatcatca
     4441 tttggttctg atagcggttc tgctccattg caacgccgta gcccttttgc tggaattccc
     4501 tgaatctctg tgaaaagagg tcgacaagtc cgaaggattt aatagtttgg aggttttgat
     4561 agctttcctc ctcaatagca aggcgttcgc cggcgagctc acggtttttt atctgaaatt
     4621 ccgtggcttt accgaaacgg aattttgcgg tcagaacagt tatcggagcg cctgccagcg
     4681 caataagagc catgagcggg tcgtagtgaa gtattataaa gaaggctccg ataaagttga
     4741 cgagtgccgt tacaacattc gggatatagc tgagcaccat atttgaaacg atgccgacat
     4801 cggcacctgt tcttgtcatt atgtctgcgg agtgaaaagc cgatattgca gtccactcag
     4861 tgttcatgag cttgtcataa acatcatttt tgattttgat tccgacattg agccgtattt
     4921 tcagcgatac acggtttgta agtgcggtga taaatatctt tgctatggct gttccgagat
     4981 acactgcggc aaccggtgcg attttcatgg aaatttcgcc ggttactgag tcgataagat
     5041 ccttgagcac ccatgatgta aaaagaccga gtccggtgga aataagtccg ataagggaga
     5101 aggctataac ctgacccttg tagcggagaa tatagctgaa tatccagctc catgagtgaa
     5161 gaattttctt tatttcattg tgattcaaat attttttgat cttttcaatg cttttcaatt
     5221 cgatgttcct aactcttttt ctactgtctt taccgcatgt atatcctttg tacattcaag
     5281 gcgataaacg ggtgtgcatt tgagcagctc atcaagtgtg cttattccca aatccttgca
     5341 tccgtcaaac cagtcgggca gcatgacctc gggtaaaacg catttcagca tgtccattcc
     5401 cttgatctcg gctattaagc atttctctga cttgacggga acgataattg cctttatcgg
     5461 cagtttttta ttcaggcagt atttcgacga accgtgccac ggcgaaccgt aggcatagaa
     5521 acttccgtca ctatccctgt gaataaaaac catgtcaccg ttgacgattt ccgcaccgcg
     5581 gtgttcctgc caaagctctg cctgcgttgt cttgccggtc cccgacggtc cggtaaacag
     5641 aattccgtcg ccgttgacgt tgataagact gctgtgcata acaattgtgt tcctgcgaat
     5701 cagattgccg taaagagcga ccatggaaca gccgtaaatt ccgttgtaat ccctgctgct
     5761 gccgaatatc gtg
//
LOCUS       DAWBSW010000131         5724 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2629_length_5724_cov_2.241489, whole genome shotgun sequence.
ACCESSION   DAWBSW010000131 DAWBSW010000000
VERSION     DAWBSW010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_5724_cov_2.241489"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2758..2779
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3841..3850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1635 a         1228 c         1441 g         1388 t
ORIGIN      
        1 gcgtaaattg aaattaggct atgcccgtgc tgcaagaatc attgatgagc ttgaacagag
       61 gggaatagta ggtccgttcg agggcagtaa accgagaaag gttcttatat ccaaacagca
      121 gtggatggag atgaacgccc tcggaaacgc ttccgctccg attgaaaatc cgattgagac
      181 ggatgcatat gaagaagacg atgaggagta aatcatgttt ttaccgataa ccgcagaaga
      241 agttaaagaa agaggatggg acggggtaga ttttgtctat gtttcgggcg atgcatacgt
      301 tgaccatccc agcttcggtg cggctataat tacaagagta atggaggcgg agggttacag
      361 agttgctttc ctcagccaac ccgattggag aaaaaacgat gattttctcc gcttcggcag
      421 acctaagctt catggtttcc tcgggaaaca ttgattcaat ggtcgctcat tacacggcgg
      481 caaaaaagca tcgaagtcag gacgcatatt ccccgggaaa agttatgggt ttaagacccg
      541 acagagccgt aattgtttat tgcaacaaga tcagggagct ttacggcgat gttcccatca
      601 taatcggcgg tcttgaagcg tcgcttcgcc gctttgccca ctacgactac tgggacgata
      661 aaataagaag gagtattctt tttgattcgc aggccgacct aatttcctac ggaatgggcg
      721 agaatcaaac aattgaaatt tgccgcagac tttccaacgg tgagccgata agctccatta
      781 cggatgtcag gggaacctgc tacgcctgcg acgtgcggga aactccgctt tacggcgcag
      841 aatgtccgtc ctttgaaaac gtttgcaaga gcaaaaagga atatgcggtt tcctgccgaa
      901 tcgaacagga cgagcaggat catataagag gtaagctcat taaacagcgc cacggaaata
      961 aaatgcttgt gcaaaatccg cctatgcctc cgctaactca ggaggagatg gactgggttt
     1021 actcgcttcc ttatgagagg acatatcacc cgtgctatga aaaaatgggc ggtgttcctg
     1081 cgattgaaga ggttgaattt tccattacac ataaccgagg ctgtttcggc gcatgtaact
     1141 tctgctcgat agcgtttcat cagggcagat ttgttacgac aagaagcaag gaatctatta
     1201 tcaaagaagc gaaaatgctt acagaaaagc cgaattttaa aggctacatt cacgatatag
     1261 gcggcccgac tgcgaacttt aggcgcacaa gctgtctcgt tcagcttgaa aagggtcttt
     1321 gtaagggcaa aaagtgcctt gcaccgacac cctgcaaggc tttgaaagcc gaccacagcg
     1381 aatatcttga tattcttaga tccgttcgcg aattgccgaa ggtcaaaaag gtgtttattc
     1441 gctcgggaat aagatatgat tatatgctta aagacccgga cgatacgttt ttccgtgagc
     1501 ttgttaaata tcatgtcagc ggccagctca aggtagctcc cgagcattgc tcggcggcgg
     1561 ttcttgataa gatgggcaag cctcatattg aggcatatat agagttttca aagaaatatt
     1621 ttgaaataac aaattccgtc ggcaaggagc agtaccttgt tccttatctc atgtcgtctc
     1681 atcccggatc aaccttaaag gatgcggttg aactggcggt attcctgaag aaaagacata
     1741 ttcgccccga gcaggttcag gacttttatc ccacaccggg tacaatttca acctgtatgt
     1801 tctacacggg acttgaccca tacacaatga aagaggtcta tgttgccaaa actccgcacg
     1861 acaaagcctt gcagcgtgcg cttttgcaat attatgaccc gaaaaacagg gatctttgcg
     1921 aggaagcgct cagaaaagcc ggcagacacg atttgatagg ctattccgag aagtgcctga
     1981 ttgcaccgaa gcgaacaaac gcagtcagag cctcaaagaa cagcttcggc aacggtaaga
     2041 gatatgaaaa aaagaagaaa aaatattaac acgaaagctg taacgggaat catcgttttg
     2101 attattgccg ttctttcggt gctcggctca cggtttttcg gtgacttcaa ggatatttat
     2161 gatgaagccg gactgaggaa cacagagcag agcaatcccg ataagatgta cgtcagcttc
     2221 attgatgtcg gacagggtaa ttgcacactg ctcagatgcg gcggcaaaac aatacttgtc
     2281 gattcgggcg aggtcggagc ggctcagacc gtcatcggct acataaagaa tcttaacatt
     2341 gatacgcttg actgtgttct tgtcactcat cctcattccg accacatggg cgcgatgaca
     2401 aagctgcttt atgaatttaa aatcaaggat tttatcatgc ctgagattcc cgaaaagatt
     2461 attccgacaa gcaggattta tgaaaagttc ctgacagccg tttcggataa tgccgagaat
     2521 gttatagccg ccgaaccggg cgagaccttt tcctacggtg aaatgactgt ggaaattttt
     2581 gcaccgctgc atgattatga cgatttgaat gatatgtcgg cggtcacgag agtcagctac
     2641 ggcgatacat cggttatgtt catgggagat gcatccgcaa cggttgaaaa agacctgctg
     2701 cagaaggatg ttgattattc cgcaacgatc ctcaatgtcg gccatcacgg cagcaagnnn
     2761 nnnnnnnnnn nnnnnnnnnc gtgccgttac aaaacgcttt gaaagcatgg ggatagaata
     2821 tttccgcacc gatttgctcg gaacgatagt ttttgaatca aattcaaagg aatttacaaa
     2881 atgtgattga ctttaatatg ccgcaaagca tataataata aattagttat taactcaatt
     2941 tacagggagt tgtatttatg gaatggacag gacttaacga attaagagaa aaatttcttt
     3001 cgttttacga gagcaagggt catttgagac tccctagctt ttctcttatt ccgcagggcg
     3061 acaaaagcct tctgcttatc aacgcaggaa tgtcgccgat gaaaaaatat tttacgggcg
     3121 agatcacccc gccgagaacg agagttacaa catgtcagaa atgtatcaga acgcccgata
     3181 tcgagaatgt cggaaaaacc gcgcgtcacg gaacatattt tgaaatgctc ggcaatttct
     3241 ccttcggcga ttattttaag catgatgcca ttacatgggc atgggaattc tgcaccaagg
     3301 taattgagat ggatcccgaa aggctctata taagcgttta tctcgatgat gacgaggctt
     3361 atgacatctg gacaaaggag ataggcgtac aggagagcca catggttcgc ttcggcaagg
     3421 aggataactt ctgggagcac ggagcaggtc cttgcggccc ttgctcggag atttattacg
     3481 acagaggacc tgagcacggc tgcggaaagc cggattgtaa ggtaggctgc gagtgcgaca
     3541 gatatgttga gttctggaac ctcgttttca ctcagtttga gaacgacgga cataatcatt
     3601 atactccgct tgcccatcca aatattgaca caggcatggg tcttgaaagg cttgcctgta
     3661 ttgctcagaa tgtcgacaat ctttttgagg ttgacacaat tcagaacatt atgaaacaca
     3721 ttgcaaaaat cgccggcgtt gaatatcaca ccgacgaaaa gagcgacgtt tcgcttcgtg
     3781 ttatcacgga ccatatacgt tcgacaacct ttatgatcgg cgacggcgtt atgccttcaa
     3841 nnnnnnnnnn gcctttcctc tccgaggttg ccgacacggt catcaatgaa aacaagaatg
     3901 cttatccgaa ccttgtcgaa aagcgtgact ttatcgttaa cgttatctcg accgaggagg
     3961 agagctttaa cagaacgatc gacgcaggct tagacgttct ctcaaaaatg attgaggatt
     4021 ctaagtcaaa ggagctgagt gccgaggacg catttaagct tcaggacaca tttggattcc
     4081 cgatagatct cacaaaggaa atacttgagg aaaagggcat gaccgttgac gaggataaat
     4141 tcagggagct tgttctcgtt cagcgcaagc gttcgcgtga cgctcacaag ggcgcaggcg
     4201 ccgagggctg gaatgatact ggcgtcgtta caaagggtat tgcaaagacc gagttcaagg
     4261 gctacgattc ttacgaagct gagggcaaga taattgcttt tatcacggac ggtatcagat
     4321 gtgacgtgct tgagaacggt gcagattcat tccttgtagc ggacaagacc tccttctatg
     4381 ccgaaagcgg cggacaggta ggcgacacag gctatatcta cggcgacggc ttcacttttg
     4441 aggttgagaa cacaacaaag accaatgacg gcgtaattct tcattacgga agaattatcg
     4501 acggcgacaa cgccaaaaca ggcgacagcg taaagactgc catcgactct cagaggagag
     4561 acgcaattgc aagaaaccac acggcggcgc atcttcttca ggcggctttg aggaaaacac
     4621 tcggctcgca cgtagagcag gcaggtcagc ttgttaacga aaacactgtt cgttttgact
     4681 tttctcattt ctcggctatg acatccgatg agctgaaagc ggttgaaaac gaggttaata
     4741 atattattct ctcggccgag agcgtcacaa tgaccgagat gccgatagaa gaagctaaga
     4801 aaacgggagc aatggctctt ttcggagaga agtacggcga cgttgtacgt gttgtcaagg
     4861 ccggagattt ctcgactgag ctttgcggcg gaactcatgt ttcaaacacg ggcaaactcg
     4921 gacttttcaa gattattacc gaaacctcgg ttgccgcagg cgtaagacgt atcgaagcgg
     4981 taacaggtac gggagttctt aattatatcg actcgcttaa ttcaaagatt ttcggcactg
     5041 ctgctgcact caaggttgcc aatccctcgg atatcgaaaa gaaagccgtt tcgctttcaa
     5101 atgagcttaa agagaaggac agggaaatcg aagcgcttac tgctcagctt accgaaatgc
     5161 gttcgggcag tctctttgac aatgccgttg aggtcggtac acttaaggtt atcgccgcaa
     5221 atgcaggtga agtaaccgtt gacgagcttc gtcagctttc cgacaaggca aagtccgagg
     5281 gagacaacat cgttgccgta ttcggcgccg tgaataaaga aaaaggctcg gctaattttg
     5341 ccgcttgttg cgctcccgag gctgtcaaga gcggagttaa agcaggtgac gttgttcgtg
     5401 cggttgcaaa gttagcaggc ggaaacggcg gcggaaagcc cgatttcgca atggcaggcg
     5461 caaaggatat agataaagtt gatgaagcaa tcgcggcggt tgccgatatc gttaaatctt
     5521 ttataaaata aatttaggag gcatagttat ggattgtctt ttttgcaaaa taatcaatgg
     5581 ggaaattcct tcgaacaaag tctatgagga cgatcgggtt tttgcattcc gtgatatcgc
     5641 acctcaggca ccgacacata ttcttattat cccgaagcag cacatcaagt cggccgccga
     5701 gatcgacgag agcaacagtg atgt
//
LOCUS       DAWBSW010000132         5625 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2674_length_5625_cov_2.436625, whole genome shotgun sequence.
ACCESSION   DAWBSW010000132 DAWBSW010000000
VERSION     DAWBSW010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_5625_cov_2.436625"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1519 a         1243 c         1330 g         1533 t
ORIGIN      
        1 ccgcttgaga aaacgggcag cggcaaaatc aagagaaaag caaccgtatt ataaattttt
       61 aaggaggcat ttatgaattc tgtaaacaag cctgtatatc cgctcattcc gccgcaggaa
      121 atgattcagt ttatgcttaa atacagcttt tttcataagc aggtcacgca gattccggat
      181 tcgataatcg tatcacagaa gattgatttt gacgttatga ccgaggcgtt taatattgaa
      241 attgagcgca atgactgtct gagacttgtt ttcttcaaac agaacggcaa tataatgcag
      301 tatttctgtg atccttacag gataggaagc gttcccgtat ataatttcaa atccgacgag
      361 gaacgtgaaa aggtgctcac ggcagatgca cagaaaccga tcaaaatgct caagggtgaa
      421 atcttcagac tgaaatattt cacaacgtat gacggcagat acggcgtata tatcaatatt
      481 caccatctcg ttatggacaa tgccgcggtt ttcgcattct ttaacgacct ttttgccgtg
      541 tatgaccatc tcaaaaacgg caagccgatg ccgaagcctc tcggaagcta tgaggacaga
      601 atcaagcgag agcttgctta tgttgaggat aagtccaatc tcgaaaagga gaaggcggca
      661 tataccgaat acataacgag aaacggcgaa ccgctttatc tcggcgttga gggacccaag
      721 cttcttgagg cagagcgtaa aaagaagaag gatccgagta tcaatgcacc gtcgctcttt
      781 gacccgattc atgataaggc ggagcttaca aagacaacat tttctcccga gcttagcgaa
      841 aagttcttct ctttctgtga gaataacaat gtcagccccg agtgccttgt tcagctcgcc
      901 ctgagaatgc acctttcaaa aatcaacaac ggtcatcttg atacatattt tatttgcctt
      961 tgcacaaggc gtcgcactct caccgaaaag cgttcgggcg gaacggtcac ggctccgctg
     1021 ccgtggagag ttcatcttga agaggacgat accttcatgt cggcactcga caaaatggct
     1081 gacgctcagg tatgggcatt cagacacatg gactatcctt atcttgagta tcgcgacctc
     1141 cagcgtgagc tgttcaatta ttccgccgcc gccggctcgt caacgatgat gttctcctgg
     1201 atgccgataa atgagaagtc gataaacggc tgggaatatg agtatgtagg ctacggtctc
     1261 ggacgctata taatggtgct ttacaccttc gctatgaagg atgcacacag cggatgtctg
     1321 aaattaagct gcctgcacag gacgaagttc gtcagcgttg aggacatcaa agctttgcat
     1381 aacggcacta agaaggttct tgaaattgca ttgaacgaac cggatatcag cattaaagat
     1441 ttgcttgaaa aaatgtaaga gaggtttatt aaaatgcttg aaacattcag aaatataatt
     1501 tgcaactatg ttgacattgc tcccgaggat atcacggagg attcaaagct tcgttcggat
     1561 atcgaactca actccttcga tatggtaaat gttgcggttg accttgaaaa tcagtatgga
     1621 gtaaaaattg acagtaagaa attcggcgga ttgaaaaccg tcggcgattt gatgagttat
     1681 atcgagtcaa ttaaataaaa ccaaagctgt gatgaaattt tgtttttcat cacagctttt
     1741 tattatatta gctttttatc tccagatgaa ccagataagt atatatcctg tcagcacggc
     1801 ggcgagggtg aagggaacgc cgtatttcat aaaggttgtt gccttgacct catatccctc
     1861 tttcttgagg attccgatgc cggcgatatt tgcgcttgcg ccgatcgggg tgagatttcc
     1921 gccgagcgtt gcgccgcaaa gcagaccgta gtaaaggagc ttcggctcca tacccatgtc
     1981 ggcggcgata acgggaacaa tggtgagcat tgtcgccgtg tagggaatat tgtcgataaa
     2041 cgccgaaaga ataacggaaa tccatactat aatcgtgtag attatgaact cggagcctga
     2101 gccgagcttt gcgataccgt tgccgataac gtcgataacg cccgcgcttt tgattccgcc
     2161 gattacgatg aaaagacctg tgagaagggc aatcgtgtag tagtcgatct ccttgaaggt
     2221 gtccttgact atcttgtaat ttttgttgaa gaaaacctca cggataacgc cgattatgaa
     2281 gaagcctatg cagataagcc cgttggtgat atcgagcttg tagaactgac cctcggctgc
     2341 cccgtccttg tagggaatga acgaagcggc gatgagaagc acgattgttc cgacgagggg
     2401 aaatgtcggg aatttatact caacctttgt tcttgcgttg aattcaatct ttgcattgtc
     2461 cttgcggaac ataaaaagaa taatgagtgc gctgacgatt gctcctgcct caacgaccca
     2521 gaacataccg ggttttccgc tgtcaacgaa gaagtccgag aagtcgaggt tagccgattt
     2581 tgcgagaaga atcgaagttg tgtcgccgac gagcgttgcc gcaccctgga taccgcaatg
     2641 caaatgattg acggaacagg ggagatgttg acctttttgc agaaggcgag ggcaaccggc
     2701 gcaatcatga gcaccgtcgc aacattgtca acgaatgcag aaacaatacc tgcgaaaagc
     2761 gaaagacaaa caattgccca cttgacgttt ggcatttttg agatgagaat gtcggacatg
     2821 agctgcggca tcttcgactc gatgaaaaga taaaccgttc ccattgttcc ggctatcatc
     2881 ataagaacat tccagtcgat ttctccgaga gcctgtgcga cgccgtagga ataagttgac
     2941 tcgccgacgt tgaagaatcc gctgccgaaa agatcgggtg aagctgaggc gatagtgccg
     3001 agagcgatga aaataagcgc agagcctatc gcaacgtatg gcctgatctt ctgaaaagcc
     3061 atcatcagta tgtaggtggc ggcgaacaga atcaaggctg tgatcgtgat tgggttcatg
     3121 gtttgacact cctaaaatta tttcgggcgg aatccgaggg caaacggctt tgcaaaaaaa
     3181 taaggcagaa tctctacgga tcctgcctga aaagaccaaa gcttaattag gtcaatataa
     3241 cccgaaacaa atagaacata atgttctatg acagactcca ccacttgctg ccgaatatct
     3301 tcggctcaat cgggttaccg tatgattata acatatttat gcggcaagtc aagcaaaatg
     3361 aatagatttt aagacttatt ttatggtttt aattgataaa atattttcaa attcaatttc
     3421 gcttctgtcg ctcaaaataa gactgcgtcc gatttcgtca attctcctga ccgtgcacgg
     3481 aacggtgatg attgctccgc cgtcctttct gccgtcgggg cggaaaaagg tcagctcaac
     3541 agccgtttcc ttttgcgggt tatcccttat ttcgagaaga atacggttga ttttttcctt
     3601 ctcggcttcc tcaagctcgg gcttggtgtc cgtcagtctt gccgtttcct ccatctcgtc
     3661 atcaagtccg actaatgcgg caaatggggc gaactgcgca gcccgtgccg agcgtgtcat
     3721 ctgcggtctt ttggtcgaaa cacggtgggg gaggttgaat atatcgtcgt attttccgct
     3781 catgccttat gacctccgat ttgtcggttc ctttcaagcg ccgttgcgcc ctccttgagg
     3841 ttcatgccct ttattattga gttcttgccg tatttactct tgattttgag aattgctttt
     3901 tgcatatcct tctctttctt cagctcgttt tcttcggctt cctgcttccc tgtgtcggaa
     3961 aaaaggttaa gctggatgta ttcgcgttct ttttcggcgt cgttttcggt tataatatga
     4021 ttggcgacga tgtacattcg acgcacgagc agctctttat caacgattct gtcaaaaagc
     4081 ttcatcgccg tttcgcaaat aagctttgtc gaggaagtgt agcgcccgat attttcgctt
     4141 gaatgggctg tctttggaat ttttctgcca tagcgatcgg tctcaaccgc acccgagtag
     4201 ctttttttca ggttttcaat gtcatagccg acagtcagaa caatctggtc ggtaacaatc
     4261 ctttttgaaa caagctctaa ggacagcagg tctgccattt ctttcagcac aagctttgct
     4321 tttgaaaact catacggttc ctgaagcact tgaccagagc tgagactgct gctctcgggg
     4381 cgataggact taatgtcgga aatctcggtc ggctcccagc cccatgcgtg gtctataaga
     4441 agctcggcgt tgactccgaa aagcttataa agcaaatcct cgttataata atcgctctcc
     4501 ctgccgaccg aacagcgggc gacgtcgccc atggtgtaca tgccgtgctc ctcaagcttt
     4561 tttgaaattc ctctgccgac acgccaaaaa tcagtcagcg gacggtgatc ccagagtatt
     4621 ctgcggtagc tcatctcgtc aagctcggct attcggacgc cgtttttgtc gggctcacag
     4681 tgttttgcat agatgtccat tgcgatttta caaagataaa ggttcgttcc tatgcctgcc
     4741 atggcggtta tgcctgttgt ttcaagcacg tcaagcacca ttttcatcgc aagctcacgc
     4801 ggcgtcaagc cataggtttt gagataatcc gttgcgtcaa tgaaaacctc gtcgatcgaa
     4861 taaacgtgga tatcctcggg agcaatatat ttcagataga cattatatat ccgggtgctg
     4921 acctccatgt aatgtgccat tcgaggcggt gcggtgatga atgtcagctc ggcagacggg
     4981 tctttaataa gatcggaatg aaaatacgat tcatggctga agctatgccc gggagccttg
     5041 tatttacgtt ggacgttgac ttcgcgcacc ttttgaattg cctcaaaaag tctcgccctg
     5101 ccggggatcc catatgattt gagggacggg gagacggcga gacagatggt tttctccgtc
     5161 ctcgtcgggt cggcgacaac aaggtttgtg tccaaagggt caagacccct gtcgacacat
     5221 tcgacagaag catagaacga tttaaggtct attgcaatat atgccttgtt cgccaccgac
     5281 ggcacctccg gatatggatt ttagatgttg acaaattata taagcggtat ttgatgctta
     5341 tctcatctaa aagctgatat ctacattata tcccgaacaa acgttcaggt caatatagtt
     5401 tttcaaaatt attgtgataa aattttaaca atatcgcgag tgcaatgtca caatgcacaa
     5461 aaagatattt gttatgttgt gtattttgtc gtataaaatc ttgacaatac gggacagaat
     5521 aattataata ttgttgtatt tacataccaa aagaaagggt gatacacatg aatgaatata
     5581 ttgaaagagt tctcgcttcc gtaagagaaa agaatgcgtc cgagc
//
LOCUS       DAWBSW010000133         5620 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2678_length_5620_cov_2.718239, whole genome shotgun sequence.
ACCESSION   DAWBSW010000133 DAWBSW010000000
VERSION     DAWBSW010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2678_length_5620_cov_2.718239"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1017..1026
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2098..2107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1427 a         1171 c         1264 g         1738 t
ORIGIN      
        1 gctttacagc ggcacgacga tcggcagaaa gaaaatttgt ctcctgccga caatggatgc
       61 gagacgatta aagctggtta tcgaaaagac aagagatttt gctacaataa caaagtttga
      121 agtatattaa caaaagaaaa taattaaaaa ggcgtttcgg aaattttccg aaacgcctga
      181 ttactttcag ctttaatcta tttcaaaatt tatttagcaa cgtctatcac gcggttttca
      241 cggataacat tgaccttgat ctgtccggga tactccagtt catcctcaat tttcttgacg
      301 atatctctgg caacgagtac catttcatcg tccgaaaccg catccggctt aaccatgata
      361 cgaacctcac ggcctgcctg aatggcatac gatttttcaa cgcatgggaa tgagtttgtg
      421 atctcttcga gcttttcaag tctcttgata tagttctgaa gattttctct tcttgctccc
      481 ggtcttgcag cggatatcgc atcggctgcc tgaacgaggc aagcaacaat tgttcttgct
      541 tcaacgtcgc cgtggtgggc ttcgattgca tgaacaacac tgtcgttctc tttatatttc
      601 ttggcatact caacgccgag ttcaatgtga gaaccttcca tttcgtggtc gagagccttt
      661 ccgatatcgt ggagaagacc tgcacgcttt gccgtcttaa catcggcgcc gagttcggca
      721 gccataagac cggcgaggta agaaacctca agtgaatggt tgagaacatt ctgaccgtag
      781 ctggtacggt atttcagctt gccgagctgc ttgataagct cgggatgaat tccgttgacg
      841 cctgcgtcaa tagcggctct ttcacccgtc tgcttaatgg tatgttcaac ctcacgcttt
      901 gatttttcat aaagctcttc aattcttgtg ggatgaattc taccgtctgc aatgagcttt
      961 tcaagggtaa gtcttgcaat ttcacggcga acgggatcaa aacatgaaac ggtaatnnnn
     1021 nnnnnnaatc gcttccggtg tgtcgtcgat aataagatcg actccggtga ttgtttcaag
     1081 agttctgatg tttcgtccct ctcggccgat tatacgaccc ttcatctcat cgttaggcag
     1141 agcaacgacg gaaacggtag cgtccgcact gtgatctgcg gcgcagcgct gaattgccgt
     1201 tgcaataatt tcacgagctt tttcgtctgc ttcatccttt gttttctgct cgtattccat
     1261 aaccttgact gccttctcgt gaacgagatc ggattcaaga gttttgagca gataatcctt
     1321 agcctgttcc ttgttaaagc ctgaaatttt ctcgagcatt tcgatctgac tgcgcttgac
     1381 aagctcaatt tcttccgctt tcttgtctgc ggctttgatc ttgttgttga gctgctcgtc
     1441 ctttttctcg atgttctcca tctttctgtc gagactttcc tctttctgag aaagtctgcg
     1501 ctcctgacgc tgaacctcgc ttcttctctc acgaagctct ttttcaagct cggtgcgctg
     1561 acggtggatt tcgtccttgg cttcgaggat agcttccttt ttcttggact cggcctttga
     1621 aacggcatcg tttacaattc tggtagcttc ctgctgtgcg gaacctatcg tagcttctgc
     1681 taccttctta cggtagacaa ttccaactgc aattcccgca gccgcagcga caacaacaca
     1741 ggcaactatg atcactacaa aaagtgatat tttgattgtc aacaaagtat agatgcacct
     1801 cctgtcttag aatttaattt aagtaaaaaa tcaataatat tatattggca ggcggctgtt
     1861 cgtttccgct tgccgacggt gttattataa tccggcaaac tacaataaca tctgtaatat
     1921 tttaatacat ttttgcactt gtgtcaaggt gaattatacg atttcaataa actgtactaa
     1981 atttaccgat tctatttatt aaaaattgat taagattttg caatttaggt tgacaagtgt
     2041 atttttttat tgtatagttg aactgcttat tattgtattt gaagtataag aatatttnnn
     2101 nnnnnnngtt gaaaaaagtt aatctcgtct tataatacag taataatgaa catataataa
     2161 gtcgtaatag tttttcatta gagtaaccgc agaaaaaacg ggggaaataa tttaatcggc
     2221 ggttatcaag agagaaaggg gtattttatg aaaaaaatct ctgaaaggat aattgcgatt
     2281 ctgctgacgg ttactattgt gttcggcacg ggtacggcaa tttcctatgc gaaaaccgat
     2341 gccgatattc cgagcggaga tgttgttttt gatgagactg acccggggta taacgccgga
     2401 aaggatcatc tgacgactca gcagtttttt gactatattg acacgatcaa tgcaatgttt
     2461 aagaaggttt ttggctttaa cattattaac gagaaaaaaa ttaaggttga agtcaacggc
     2521 gttatgaacg acatttttac cgagtggacc cgtaaaagcg gcggcacact cgacatcatg
     2581 aagatagtca attctcttcc ggattttaca gacggttcgg ataaggtttg cgattttttg
     2641 agaattgacg ttgatacgct tgcaccgatt atgaaggata aggcaaatga gctttataaa
     2701 aacggaaaca ccttaggggg acttttcgtt gactttgcaa gaatttatct gcttcaaatc
     2761 gattatatga cgctcgatgc ggatcagaac aaaaatgatc ctacaatcta taatgttttc
     2821 attgtaattt attataagaa cggtaaatcc gagaagatca agtccgattt tgtttatgac
     2881 acaaccactc atgagcttaa aagaaaaaat gacagaggtg ttttcggatt taatattgat
     2941 gccgatgatt atgttatcta cgcgataaag aatacctggc agagaaattt cggcttctgc
     3001 atagcgtacg atatccttgc taaccttacg atttatgatt atgatacgaa gcgtatctat
     3061 ctcacctacg gcggcaagga ctggcttttc caaatttgga agggaattta ctggatttcc
     3121 aacggtgccg agataggtat ctataaccgt ccggagcaga aggtttggac gacggtgttc
     3181 caatgcgcac ccgatgagga tatgatggta atgagtcttg agctattcca gggcagcaga
     3241 agaatttttg tccgtgaagc tcaacttcat tggtgggtca tcggtttttc aattgaggaa
     3301 aaggtttacg acggcaagta tctcaccgca aagggtacga tagaattccc cgatgaggat
     3361 tttgccgaga tgtttgtcaa tgccgcaaac aagaagggcg ttgtttcaag ccgtgacggt
     3421 gtgaatgttt cctggtgctg ggcgcagggc acataaaagc ttaacataaa aatttgagac
     3481 tgtctcgttt gagacagtct cttttgttat gctttatttt gtcttaggga attgtgtttt
     3541 atcatagccc atgacccttg caagctgagt gcttatgcaa taattgtcat aagcctcgcc
     3601 tgtgataaat cctgcgtcgt tcgctgaaac gtaaatagga acgttgactc ccgtgtgttt
     3661 ttttgtcgtg tagtagtatt cgccggtttc ctcattatag gtaattccgc cggtttcgtg
     3721 atccgctgtt acaagaacaa gcgtatcgcc gtcctgtttt gcaaagtcat acgcaacctg
     3781 aattgccttg ttgaattcaa taacctgtgc cgttgagccc tcaagaatat ttttgtgtga
     3841 gaacttgtcg atgtgtgctg cttcgaccat gaggaaaaat ccgttttcgt tgctgttgag
     3901 aagctcaatt gctttcttcg tcatatcggc aaggtatggg ttgtcgttgt tgtttgtaac
     3961 atttgcaaaa gcatccatat caaactgggc gaatgagcgt gtgccgggtt gaagtgcgtt
     4021 catttctttt gcacttgaaa tatattgaaa tccgccgagt ttgcagccga gctttgtgac
     4081 ggttgtactc ttagatcccc agatgaggtc aagctcggag tgcatctgct cgaccgaaat
     4141 atcaggctca aactgcctta taaatgaatg agccgagaaa gacgcgggag ttgcgccgct
     4201 cgttttatct gttgttacaa cgcctgccgc ttttccgttt tctttcgcaa gctccgttag
     4261 cgatttcggc gtattgaatt tctttgcgaa tggggcaaag gcataaacgc cgactgcatt
     4321 attgtaggtt ctaacaccgc aggaaagagc cgttgcacct gccgcactgt cggttatttt
     4381 gtttgatgca ctgcgtgttt ttgactggct gtgaacagga aaagtttcca tcggaagctc
     4441 atcaatgccg tatctgcttt tggcagcttc gaccgtgtta aaacccattc cgtcgccgat
     4501 cataaggata acatttttgt aggttttgta ttcgttcttt gttccgtccg tgccgtaaaa
     4561 tccgagcata gtgcttatta aaagcaaaac ggtcgttata atcgaaataa aagatttaat
     4621 tccgcgtatc atatttacct ccataaacca acagccgctg aaaaggcttc aacggctgta
     4681 acaattctat tttagtggat agactttata agctcttcaa aagcgtttat tgttgtagag
     4741 cctgcaaggt tacctgttgc aacgatttcc tcgttgactc ccgagaggat actgctgtag
     4801 tcgttatcct caagtatgta accgatctgg tcgttcatga gaccgaatac aagaagctct
     4861 ttgtctgttc cgacgatatc ctgaagtgaa ggatagttga aatcggtgcc tgtccatgag
     4921 tggtcggcgt caagatatcc gccgtacgcg attgccggct caagctctcc ggggattatc
     4981 gcaactgcaa gttttgtgcc gatttcaagg taaccaagct ctgttgcgac ctcaagaaca
     5041 ttgttgttct catctgttgt aacaacggtg tttgtgaggg ctccgagtct acctgcaaat
     5101 tcaaggattt gatttgttac gggaacatag tattctttca tcctgtagtt gaggagggga
     5161 gcgacttctg tttcggcttc gttggactca acaatgagct tgccgagtgt tgtaccgtaa
     5221 atcttgagag attcaagtct cggtgttcct tcgggaaggt taagactgtc atgttttgaa
     5281 gagattgcaa gctccgcgcc ctgaatctga atgaagtttg caccggcctt gttgacttcc
     5341 tgctcgatat agtatgggta gtcgccggtt atctcagttc ctgccgcgcc gttgccgaca
     5401 gcatggatag ccatgttgca aagccatgtt tcctttgtac cgttatcggg aacaaagcgg
     5461 aagcggtgaa tcttcgagtc gaaaaccatg ggttcacgct tgtcacggat atattcgccc
     5521 gcttcaatct cggaatagta cattttaccg gttgtcattg aattgacagc ctcttttacc
     5581 gagtcaacgg tgacgtcata aagatgcttc atgtagcttg
//
LOCUS       DAWBSW010000134         5564 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2701_length_5564_cov_2.666546, whole genome shotgun sequence.
ACCESSION   DAWBSW010000134 DAWBSW010000000
VERSION     DAWBSW010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_5564_cov_2.666546"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    706..715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2706..2715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1584 a         1174 c         1426 g         1360 t
ORIGIN      
        1 gcaagctaaa gtattgctca attcctgaag taaattaaat cacggcggaa atcggttaat
       61 ttcgatttcc gccgtgttat tttccgaaaa ggtgttgact ttatcgggta gttgtggtat
      121 tatacatcaa aagaaatctt taagagcgat acagagatgt cgacaaggtt ttgaatagtg
      181 agacggtagg gtaacagccc gtgtttttgt gcgtccggat ttatgaacct tgtcccaaca
      241 gggttctttt tttattgctc attcaggagg ctctcatgac ggaatacatc ttgaaaaatt
      301 cattggtcta tacggattcg ggatttcaga agcaggatat atttgtttcc gaaggccgtg
      361 ttctttgtgt ttgctgtaag ctttgtgccg aatataccca ttcctatgat ctcacaaata
      421 agcatatttt tcccggtttc gtagatgttc atgttcatct tcgagagccg ggtttttttt
      481 ataaagagac aatcaaaacg ggttcacttg ccgccgcaaa gagcggttac acaacggttt
      541 gcacaatgcc gaatcttgat cccgtgccgg atagtgcgga gcacctcgaa aaacagcttg
      601 atattatcga aagggatgct tcgatcaacg tcattcccta cggttcgata accgtaggca
      661 ggaagggcac ggagctttcc gatatggagt cgcttgccga taaggnnnnn nnnnnccgat
      721 aaggtggttg ccttttccga tgacggcagc ggaattcaga cagaagaaac gatgcgccgg
      781 gcaatgctgg aagcaaaaaa gctcggaaaa ataattgtcg cccattgcga ggacaacagt
      841 cttttggacg gcggatatat tcataagggc gcatatgcaa aagctcacgg tcataagggc
      901 attagctccg agagcgagtg gaagcagatt gagcgtgacc tgagactcgc cgaggaaaca
      961 ggctgtaaat atcatgtatg tcatatctct accaaagaaa gcgttgagct cattcgtaaa
     1021 gcaaaacgca gaggcgttga tgttacctgt gaaacagctc cgcactatct tgttatgacc
     1081 gatgccgacc tcaaggagga cggcagattt aagatgaatc ctcctctaag aagtgcagag
     1141 gacagagagt ctcttattga gggtatatgc gacggaacga tcgacatgat tgcaaccgac
     1201 catgctccgc attccgcaga ggaaaaatca aaaggactta aggacagcgc tatgggtatt
     1261 gtcggacttg aaaccgcttt tccgattctc tacacaaggc ttgtcaaggg cggaattatc
     1321 agcttgcata agcttgtgac tctaatgagt gttaaccctg ccgagcgttt cggcattaag
     1381 accggctttg aaattaagga gccggcaaat ttcacggtat atgacctcaa tgaaaaggaa
     1441 acggtaaatc cggagaattt tacttcgatg ggaagaagta caccgtttga gggtatgacg
     1501 gtatacggca aatgcaagaa aacggtttgc aacggcaaaa ttgtttggga ggatgaagaa
     1561 tgaaccgggt agtaatgaaa ataacaggca acaaaaaaat tgccgaaaaa acatatttca
     1621 tgtcgcttga aggcgacact tcggcaataa caaagccggg gcagttcgta aatataaagc
     1681 ttgacggatt tttcctccgt cgcccgattt cggtttgcga ctgcgaggaa ggaaagcttt
     1741 cgataatata taaggttgtc ggcaacggaa cgaagaagat gtccgaactg cctgtcggtg
     1801 atgagcttga cattctcagc ggtctcggta acggctatga tacttcgaaa agcggcgatt
     1861 gtcccgtgct tataggcggt ggagcgggcg ttccgccgat gtttatgctt tgcaagaagc
     1921 tggttgccga aggcaaaaag gtaacagtga tactcggctt caactccgaa aatgaagtgt
     1981 tcggaattga tgagttcaag gcgacgggcg ccgaagttca cgttacgacg gttgacggaa
     2041 gcgtcggaac aaagggcttt gtaacagatg ttttgaaaga agcggagtat acttattttt
     2101 acacctgcgg tccgatgccg atgttcaaag cgattgaagg cgtcgcaaaa acaagcggac
     2161 agtacagctt tgaagagcgc atgggatgcg gcttcggggc gtgtatgggc tgcacatgca
     2221 aaacaaaata cggcaacaaa cgaatttgta aggacggacc tgttcttgaa agggaggaaa
     2281 tagtatggta acaaaggttg acctttgcgg aattgagctt gataatcccg tcattcctgc
     2341 ctcgggtaca ttcggtttcg gctatgaatt tgcagagctt tatgatataa atatgctcgg
     2401 tacattctcg ttcaagggaa caacaaagga tccgagattc ggcaatccta cgccgagaat
     2461 cgccgagtgc acggcaggca tgataaatgc cgtcggtctt cagaatccgg gcgttgacaa
     2521 ggttataagc gatgaacttc cgaagcttaa aaaatgcttc aacaaacctg taatggcaaa
     2581 tgtaagcgga ttttcgattg atgactatgc ttacacctgc gaaaaactcg acagagagga
     2641 gcaggtcggc tggcttgagg tcaatgtgtc ctgccctaac gtacacggcg gcggaatgag
     2701 cttcgnnnnn nnnnncgcca aggcttgcga ggattcggga gcggacgaaa tcagcctgat
     2761 aaatacaatg ctcggaatga gaattgatct tcgccgcaga aaacctgtga ttgcaaacgt
     2821 gatgggaggc ttttcggggc cggcaatttt cccgatagcc gtcagaatgg tttaccaggt
     2881 ctcgcaggcg gtcaaaattc ccgtcatcgg aatgggcggt gtttcaagtg ccgacgatgt
     2941 aattgaaatg atgctcgcag gagcaacggc ggttgaggtc ggtgcggcaa atttggttga
     3001 cccgtttgca tgcaagaaca taatcgaaac acttccgaaa gcaatggaag aatacggaat
     3061 aaataatctt aacgaaataa taggaggtac gcacaatggg taatgacgta atcgtagcct
     3121 gcgatttttc aagcagggag gagacaatgg cctttcttga caatttcaag ggcagaaaac
     3181 cgtttgttaa aatcggaatg gagcttttct atgccgaggg acccgatatc gtaagagaga
     3241 taaaggcgag aggacacaag atctttcttg acctcaagct tcacgatatt ccgaacacgg
     3301 tgaagaaatc aatggctgtg ctttcaaggc ttgatgtcga tatgacaaat cttcacgcag
     3361 gcggaacaat cgcaatgatg gaagcggcaa ttgagggtct cactcgtccc gacggaacaa
     3421 ggccgctgct cattgccgta actcagctca cctcgacgag cgaggagaga atgaaaaacg
     3481 accttctcat taacgaaagt atggataagg tcgttatgca ctatgcccac aatgcaaagc
     3541 ttgcaggact tgatggcgtg gtatgttcct ccttggagtc gggcaaggtg cacgaggtct
     3601 gcggcagcgg atttcttacc gtaactcccg gtgttcgctt tgcagacggt gacaagggcg
     3661 atcaggtcag agttatgacc cccgaacagg caaagaaaat cggctcggac tacattgtag
     3721 tcggtcgtcc gataactgcg gcggctgatc ccgttgcggc atacgaaaga tgtataaacg
     3781 aatttgtaga ttaaggagca gatactatga cagatattca gattcaaatt gcaaaagacc
     3841 ttctttcaat taaggcggtt ttcttccgtc cggatgagcc gttcacatgg gcaagcggca
     3901 tcaaaagccc gggttattgc gacaacagac ttaccctcgc ttacccggag gttcgtacac
     3961 acgttgagga agctcttgct aagacggtta aagagttcta tccggaggct gaggctctta
     4021 tgggtacttc gaccgcaggt atcgcacacg ctgccatcac cgctcagata atgggtctcc
     4081 cgatgggcta tgttcgctcg ggagcaaagg atcacggcag aaaaaatcag atcgaaggca
     4141 agcttgaaaa gggtcagaag gtcgttgttg ttgaagatct tatctcaacg ggcggcagtg
     4201 ttattgaggt cgtaaacgtt ctcagagaag caggagcaga agttctcgga attgtttcta
     4261 tctttaccta cggtatgcaa aagggtcttg accgtcttgc tgcggctgat gtaaaaaatg
     4321 taagccttac taattttgat gtaatcgccg aaattgcggc tcaggaagga tatattgcgt
     4381 cgacagatgt cgaaagactg atagctttca gaaacaatcc gtccgatgaa agctggatcg
     4441 gaggcacaaa atgagaagtg ttataagcat tcttgatctt tcggttgaag agatagatga
     4501 actcattgcc ctcgcaaatg atataatcgc aaaccccgat aagtatgcgg aagtatgccg
     4561 cagaaaaaag cttgcaacgc tcttttttga accgtcaaca cgcacaaggc ttagctttga
     4621 ggcggcaatg tatgagctcg gcggtaacgt tctttcaatg gcggatgcaa agtcaagctc
     4681 agccgtcaag ggcgaaagcg tagccgatac ggttaaggtc atttcctgtt acgccgatat
     4741 aattgccatg cgtcacccca aggagggcgc accctatgtc gcttctctca atgcgtctgt
     4801 tcccgtaata aacgcaggtg acggaggaca caatcaccct acacaaactc ttgccgatct
     4861 tctcacgata agccgtgaaa agggacactt cgataacctg actgtaggct tctgcggcga
     4921 ccttaagttc ggcagaaccg tccattcgct catcgaagcc cttgcaagat acaacaacgt
     4981 gaaattcgtt ttgatttctc ccgaggagtt gaagcttcca agctatgtca agaacgatgt
     5041 tatcaaggcc aaaaaccttg aatatattca gacaaccgat ctggaaaagg ttatgcccga
     5101 gcttgatgtt ctctatatga cccgggtgca gaaggaaaga tttttcaacg aggaggatta
     5161 tctccgactc aaggacagct atattctcac tcccgataag cttgcaaacg caaaacccga
     5221 tctgtcaatt cttcatccac tgcctcgtgt taacgaaatc agcgttgctg tcgataaaga
     5281 tccccgtgca tgctatttca agcaggttct caacggaaaa tatatgagaa tggcactcat
     5341 tctcaagctc ctggaggtag aattatgaac atagattcca tcaaaaacgg tatagtgatc
     5401 gaccatatta aggcgggaaa aggtatggag atttacaatc tcctcgagct tgataagctt
     5461 gattgctcca ttgccatcat aaaaaatgta atgtcacgca agctcggcaa aaaggacatc
     5521 attaaaattg actccgatta cgacgttgat cttgatgttc tcgg
//
LOCUS       DAWBSW010000135         5525 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2715_length_5525_cov_2.956307, whole genome shotgun sequence.
ACCESSION   DAWBSW010000135 DAWBSW010000000
VERSION     DAWBSW010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2715_length_5525_cov_2.956307"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5276..5285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1456 a         1242 c         1177 g         1640 t
ORIGIN      
        1 gccgtagccg aggctccaaa ccgaatttgc tgcaggagag gtgaggtaat cctccatgcc
       61 ttcaaagaac atatcgcttg tgaagttttc cttctgctca aacttaaccg acgggatatt
      121 tctgttaatg ctctttacga gggcatctat tatcttatca gccagattgt acagagactt
      181 gagcatcatg ccgccaagct cgtcccacga tgtgggaatc ttaatgccgc caaagtcaac
      241 acttgtgacc gcaacgttat catttgtcat tgtatctgcc gcaaaagcac tgctcgttaa
      301 agcaacgctg aaaacggtaa ataccataac gatacttaga agcaatgata ttactttttt
      361 cataatatat cacctttctg aataatattt tacagatata attttatcat aaaaaaagca
      421 tttttacaac agaaagagta ttgtgaaaaa ttaccgaaaa agggagacga tttttgtatg
      481 ggttgttcta tagctgagtt gaggaacaag gaggtcatat gtaaactgaa cggaacaaga
      541 ataggaaatg tggacgatgt ggagcttgat ataaataacg gacgtcttgt ctcgataata
      601 atttacggca aggggaaaat aatgggcgtt ttcgggaaaa gtgaggatat tgtaattccg
      661 tgggaggaca ttgacgttat cggagaggat acgatactgg tcaactgtga atgtcccgtg
      721 cctaaaggca gggcgtaaaa agcatctccg aaatcagttc aaagcaagag gaaagatcaa
      781 acagaaaagg ctataattca taaatgtctg ttaaataaac tgacaaaata tgaattacag
      841 cctttttata attgtaaata ctttgctctt ttcagcaatt ctgaatctaa gctcaacggg
      901 cttttatgta caggttttaa gtctgttttt atattcatcg cgtttgcttt cgatgacatt
      961 gttcttgttg agattgaggt taagaatttc cctgacctta aaaaagtcct ccgtcataaa
     1021 atcgcctgcg ataatattgg cacgggcgag atatctcggc gtgttagcta ttgtgttaat
     1081 gagcgccttg aaatgccagc gcaaggcttt gtcaagcttc cgcggaaaaa caacgccgat
     1141 atcggttccg aggttcggcg tttgctgagt ttggaaccag taaaaaccgc ttgtatcatt
     1201 tgtgtcaaaa aattcggttg cctcggttac aaattcgtga gcatgcttgc cgtgacgctt
     1261 cttttcccat ggaagaatgt gcgggcagtt gacgctgtac ttgtactcct cacagtagtt
     1321 aatcattatg taacgcttgc ctgattttct catgtcgccc attgtaacgt cgccgatatt
     1381 ttcaaaatcg gtaaacgcat atttggacgg ctcaatcgtt ctttcaagaa tctcgtcaac
     1441 ctttttgggg tcggcatgaa agcgcagatt gaaaggtccg agtttctgcg gctgatattc
     1501 tctgatatca agaataaaaa attcgctcgg atgctcgtct ataaccttgc ggaacttttc
     1561 catttcaggc tcaaaaggca agccctttgt tatgccgtgg cagaaaacta tttgaccgtt
     1621 tttatctgtg tcaatacgca cgcagaaatg acggatgccg tagttgaact gctgggttag
     1681 atcgccgtcc tgacagcagg cgataggctt catcttgaag ctgccggcgt tgtgtgcgct
     1741 cggcattacc acattgcaga gcttgacatc atctttaatg tatgacatcc attcggagta
     1801 aatcattttt tcacttcctc agatattgta gcaaatattt atcttgtgcc tgagaacttc
     1861 ttttatattg tcgattgcgc cgcgcatata aattttcggg tcgaaaactt cgggatcctt
     1921 ggcaaattcc gctttgattc cgtttgtgta agcctgacgg agctccgtcg caaaatttat
     1981 cttcgtgatt ccggcagcaa tgcagtcctt aagaacctca tcggaaagtc ctgatgcgcc
     2041 gtgaagaacc aacggtgtgt caattgcagc gtggatttcc tttatgcgct caatgttgat
     2101 atgcggtgta cccttgtaaa caccgtgagc ggttcctacg ccgattgcga gagtgcttac
     2161 gtttgttctt ctgacaaatt cttcggcttc gctgaccttt gtgtagctgg cttcgagatc
     2221 ggtatcgcct tcctctttgc cgccgatcgt accaagctca ccttcaacgg agacgtccat
     2281 ggggagtgca aagtcagtga cttctttcgt gaactttatg ttgtcctcaa aaggcataag
     2341 gcttccgtca aacataacgg atgaaaatcc gccggcaacg catttctttg ccagagcaag
     2401 actgctgccg tggtcaagat gcaaagcata gtccacgggg caatcagcca taagctcacg
     2461 catcatggcc gggtaaatcg caattccgcc gtagttaagc gttctcggaa tcatctgaac
     2521 aatgacggga gtgcgcatct cgtgcatgat ctcggaaacc gccattgccg attcaagatt
     2581 gtcaatattg aatgcgggaa tcgcatagtt ttctttttcg gctcttttca tgagtcttgc
     2641 cgaagaaaca accatataaa acaccttcgt ttcttgaatt tagatttatt ctttaaagtt
     2701 aattattttc caaccgtcgg caatatatac aatgcccgaa gcaacggaaa ttattgcggt
     2761 tattcccatc agaatattcg atataaggct catattgaag ccgagcgaat aaaccgacga
     2821 aacctcacag agaagcatga caacgacaaa ggagaccatt tgcgtaacgg tttttatttt
     2881 gccgcctatg tttgcgggaa taacaacatt ttgcgccgcc gctatcattc ttaatgaggt
     2941 tatcgcaaag tcacgggtaa gtattatcat cactatccat atgctgcaaa gtccatcctt
     3001 catgaatgcg agcagaccgg ccgttacaag cattttatct gcaatcgggt caagtaactt
     3061 gccaaaattt gtgactatat ggttttttcg cgcaattcgt ccgtccagcg catctgttat
     3121 tgcaccgagg gcgaaaataa ttgcggaaat caaaaattta tgttcaatgc cggcaagata
     3181 tatcgcaaga aaaacaggag caataaacat tctgccgatt gtaattttat ttggtaaatt
     3241 cattatgata ccatgcctta taatatactg taggtaattc ggtcaggctg taacctcgcc
     3301 gatgaggtcg taatcctcaa catcaaaaat ttcaacgtca acaaagtcgc cgatatcaag
     3361 atccttgtga gacgtgaatt ttacaagtcc gtcaatctcg ggggcatcca tatatgttct
     3421 gcccgtgtag ctgtcggaat atccgtcgta gtcctcaaca acaaccctgt atgttttgcc
     3481 gattttttca ttattctttt cttccatgat gctgtactga tcctgcatta tgacctcgcc
     3541 gcggcgtgtt ttaatatcct catctacctg atcctccatg tcgaaggccg gagtgccttc
     3601 ctgcggagag aaggtgaata cgcccagacg gtcaaattca atctcgttga caaattcggc
     3661 aagtgtttca aattcttctt cgccctcacc ggggaatccg acaatgaagg tcgttctgat
     3721 gacgacatcg gggatttctt ctctgagctt ggagataaca ttgcgaataa gcgcctgatc
     3781 gcctctgcga ttcattgcct ttaggatttt atcgtcagcg tgttggagcg gaagatcgat
     3841 ataattaaca accttttcct gatttttcat tgtttcaata agctcgtccg taattctgtc
     3901 cgggtagcag taaagaagtc ttatccattc gatagaatca attttgcaaa gctcgttgag
     3961 aagctcgggc agtttaagct caccgtaaag gtcaagaccg tagcttgttg tatcctgggc
     4021 aatgagaata agctctttga cgcccgattc ggcaagctgc ttggcttcgt agataacgtt
     4081 ctctatgctg cggctgcgca tattgcccct gattgacggg atagtgcaat aggtgcagcg
     4141 gtttgaacag ccctcgccga ttttgagata tgcccaataa tgcggcgtgg tgagaattct
     4201 ctgaccttca agcggaagat cgcactgcgg cggatatttc tcgattgtat caacgccgga
     4261 catgacctct tcaactgttt tgattatatc gccgtttgcg ccgatgccga taacggcgtc
     4321 aacctcgggg atctccttaa caatctcgtc cttgtatctt tgcgcaagac agcctgtgac
     4381 aataattttg ctgataacgc cttccttttt caattcaacc atgtcaagaa tattttcgat
     4441 ggcttctttt tttgcatcat caatgaaacc gcaggtgttg acgataacgg catcacagcc
     4501 gtcaacgtaa tcaacgattt cccagccctc ggcgtccatg agcgcaagca tatgctcagc
     4561 atcgacctga tttttcgggc agccgagaga gatcatacca atcttgtaat tcataaaatc
     4621 acctttagaa atttaatagt cctcataata ttcatcgtcg gaaaactcgc tcattgcttc
     4681 acggatatcg gaaaatgaat atcccattct ttgcaaagcg gaaaaaactt tttttcgtcc
     4741 gacttcgtca ttcattctat tataatatct tttgccgata atatcaacta ttctttgaac
     4801 attatccggt tcttcttctt caaaaagttc ctcaacggca gcatttgcaa tgtcggcgga
     4861 gattcctttt gcccgaagct cggattttat acgcatttta ccgaattttt tgcgttcaaa
     4921 aagttcacgt gcatagtttt cggcatagcg ttcatcattg acaaggtcaa gctcaatcaa
     4981 tttttcaact gcctcgtcaa catagtctgc atcgtgcttg cgcaacagct ttgctcggat
     5041 ttccttctcc gaatggtcac ggtaatcgag actgttcagc gcactgttaa aagcacggcg
     5101 agagcctgcc gcctcctcaa aggcagtaag ctcctcctca tttatttcat ctccggagac
     5161 taacccgcag gaaaaccaga aaatctcatc aaccgtcagc agatattctc cgtcgatata
     5221 gatatgaatt ttatccttgc gtcagatatt agcagtaatt ttcattattc gtcatnnnnn
     5281 nnnnnagctg aaaccttttc ggcaggcgct tcatcggatg cggaagattt tgcagccccc
     5341 ttgccctttg agcctttctt cgtagagccg gacttgcatg cttgaagcat tttatctttg
     5401 tcggcataga ccttctcttt gatttcgtca taaagagcct gatcgttttt caaaagctcc
     5461 ttgacgtttt cacgaccctg tccgagacgg gaatcaccgt aggagaacca tgcaccgctc
     5521 ttgtg
//
LOCUS       DAWBSW010000136         5463 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2745_length_5463_cov_2.568232, whole genome shotgun sequence.
ACCESSION   DAWBSW010000136 DAWBSW010000000
VERSION     DAWBSW010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2745_length_5463_cov_2.568232"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4727..4736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1504 a         1123 c         1291 g         1535 t
ORIGIN      
        1 atattacagg gaaccgtgtt cacgatgata tcaaaatcgg cggcttcttt gcaaaattct
       61 ttaaatccga ttgatttaag cccttcgttt tcggcaagga cggcgctgaa cgggtttctc
      121 gttacaacgg ttgtgtcggc accgttcgcc cgaagcgatt tgcatattgc tctgccgcat
      181 tttccgtaac cgagcacggc ggatttgagt gtataaatat ctctgtcggt gctttcgagt
      241 ataattctca ttgttccctg cgccgtgaga cgtgaatttt ttatgagaaa gcttttatta
      301 taatagcttt tgcctttttc accgaacgga ttgttaccga gatttccgta aaaaacgaac
      361 tgatcctttg aaagcttatc gcaaagctcg ccgacggata tcccagtccc cgaaataaca
      421 ccgttcgcaa ccgtgggtat aggcaaaatt atgctgcgat aatgcgaaag cttttccttc
      481 aatgactcgc cgtcacagca tgaggcttca aagccgagtt cgttaagcct gctgcggcac
      541 gcctgcatct ttttgtcgct gcctattaca agaaattttt ctgcctgcat tttacggtca
      601 ttcctttcat tgtattgtga agtgcagagc acactatact tctgcctcca ctgtatcata
      661 tgacaaaaaa ttgtgcgggt gctaaaatat ttcaatttgc tatttatttt tttaccgtta
      721 tgccttataa ttatagcgta aagactgatt aggagatgat atcttgtacg agatagatga
      781 attactcaaa aaggttaaaa gagttcattt tataggtatc ggcggctcag gtatgtgtcc
      841 gcttgccgaa atattgcaca aggagggcta tgagctttcc ggttcggaca ataatgagac
      901 cgacacgctc gcaagggttc gcagtctcgg aatccccgta actctcggtc agaaagccga
      961 gaatatagac ggcgctgagc ttattgttta cacggcggcc attctgcccg acaatcccga
     1021 gcttgttgcg gcgaaaaatt cgggaatccc gactgttgaa cgcgctgacc tgctcggcgc
     1081 gataacaagg ctctattccg actgctactg cgtttgcggc actcacggca aaaccacggt
     1141 ttcgtcgatg ctcactcaga tcatgattca gtgcgacaag gatccgtcgg cagtaatcgg
     1201 cggcgagctt ccgcttatcc gcagccacgg cagggtaggc gagacgcaga atatggtatg
     1261 cgaagcgtgc gaatttaaag atcatttttt aaagctggat cccgatattg caataattct
     1321 caatgttgac gcagatcacc ttgaatactt caagaacatt gagaatatta tcaaatcctt
     1381 caacaggttt gcaaaaatgg cgaacaaggc ggttatttat aacggcgacg acgctaacac
     1441 tctcaaggcg gttgccgacg tcggcggtaa ggatatgata accttcggac ttgataagtc
     1501 caatgatttt tatgccgaaa atgttgaaat ggtgaatgcc ttcgctcatt tcgacgctta
     1561 caaaaagggc gagaaaatag gcagcgtaaa gctgaaggtt ccgggaaagc acaatgtcac
     1621 caatgctctt gcggctcttg cggcggcgga ttactgcggg attccgtttg acaaggcgat
     1681 agcttctctt gagcagtttg gcggagcgtg cagaagattt gaaattctcg caaagctcaa
     1741 cggtattacc tttgccgacg actatgctca tcacccgacc gagctgagag ttacgcttga
     1801 ggcggcaatg cagatgaaat acagaaaggt ctgggcggtt ttccagccgt tcacctattc
     1861 aagaacaaag aagctcatgg atgattttgc cgaggtttta aaaattcccg acgagtgcgt
     1921 tatgaccgag atcatgggtt caagagaggt taacacagag ggcgtttaca cctcacagct
     1981 tgccgcaaag attcccggca gcgtatggtt caatacattt gatgaggttg ccgattatgt
     2041 tctcgctcat gctcagagcg gtgatcttgt aataaccctt ggctgcggtg atatctacaa
     2101 agccgctaag cttatgataa agagacttga aaacgaaaaa taatccgaca gcaaaaactg
     2161 aggattacat gtgaaaagaa gattaaatcc gtactccttg taattgttat actacaattg
     2221 tttaagaatg taaggctttt atgaaacaga gtgtggaatt aagtcggaat tcttgacaaa
     2281 tattgttaac ggggctacaa tgaagttgac ggcaaaacgt taatattatt aaggagatgg
     2341 agcattatga tggcaattat tgctttgatt tcggatttta ttgatttggc tattaaggcg
     2401 atcggatcgg tttttccgga tgctgcgcag gctattcagc aggctctcag tcagcttttt
     2461 gatttcatca catcaattat cggctgacgc agggcagctt tgctttaaac gtggactcct
     2521 atggagcagc aggaagctac agcgcctcat tagcttcttc agattttaat atcaaattat
     2581 atttataaat tttgcctttt gccgcacttt tttcttttta ggtgttgaca aagggcattt
     2641 tttatgtata ataataacag taatgattac tattatttat tgcttatatt gagcagacaa
     2701 taaatttatg cgccgatgtt taaattatcg gttaatacta attatgatta tgaaaaagtg
     2761 aagggagttt cagctatgaa tattacaaag gacattaaat acgttggtgt taatgaccat
     2821 gatatcgacc tttttgaggg tcagtacgtt gttgaaaacg gaatggcata taattcttat
     2881 gttataattg atgagaaaat cgctgtcatg gatacggttg acgcacggtt ccgtcacgaa
     2941 tggcttgata atcttgccga tgcgcttgac ggcagaaagc ccgattatct tatcgttcag
     3001 cacatggagc cggatcattc ggcaaacgta atgagcttca tcggcgctta tcccgaggtt
     3061 aaggtcgttg cctccgcaaa ggcttttgca atgatgggtc agtttttcgg cgacgatctg
     3121 agcgaaagac aaattgttat ttccgaaggt tcaacgcttg agcttggtaa gcatacgctc
     3181 aatttcatag ctgcccccat ggttcattgg cctgaggtta tggttactta tgattcgtac
     3241 gacaaggtgc ttttctccgc cgacggtttc ggaaagttcg gcgcactcga cgttgatgag
     3301 gactgggctt gcgaggcaag acgctattat ttcggcattg tcggaaagta cggcgttcag
     3361 gttcagaagc tcttgcagaa agcgagcgca cttgacatcg aaaaaatctg tccgcttcac
     3421 ggtccgatcc ttacggaaaa tctcggatat tatctcggac tgtataacac ctggtcgtca
     3481 tacggcatcg agagcgaggg aattgtgatt gcatatacct cggtttacgg caatacgaaa
     3541 aaggctgttg aaattcttgc cgataagctc agagcaaacg gctgtcctaa ggttgttgtg
     3601 aacgatcttg cacgctgcga tatggcggag gccgttgagg acgctttccg ctatggtaag
     3661 ctcgttcttg cgacgacaac ttataatgcg gatattttcc cgttcatgcg tacttttatc
     3721 gagtctctga ccgagaggga gtacaagaac agaaccgttg ctttcattga gaacggtaca
     3781 tgggctccgc ttgcggcgaa gataatgaag ggcatgttcg agaaatcgaa aaacatcaat
     3841 ttcgttgagc ctgttgttca tatccgttcg gcgttgaacg aagcctcatc aaatgagatt
     3901 gatgagcttt caaaggagct ttgccgtgat tatattgcac aggacggcga aacggctgac
     3961 aaaaatgacc ttacggcact tttcaatatc ggctacggcc tctatgttgt aacgtcgaat
     4021 gacggtaaga aggataacgg acttatcgta aacacggtaa cgcaggtcac gaattctccg
     4081 aacagaatcg cggttacgat caataaggac aactattctc accacgtaat caagcagaca
     4141 ggcaagatga atataaactg cctttcagtt gacgccccgt tttccgtgtt tgagaatttc
     4201 ggattcagga gcggcagaaa tgtaaacaaa tttgagggca gcgaacctct gcgttcggat
     4261 aacggacttg cttttctgcc gagatatatc aactcgttca tgtccttgaa ggttgagcag
     4321 tatgttgatc ttgacactca cggaatgttt atctgctcga ttaccgaggc gagagttatt
     4381 tccgatgccg agactatgac ctacacctac tatcagaaga atgttaaacc gaggccgcag
     4441 acggaaggca aaaagggctt tgtatgcaag gtctgcggat atatctatga gggcgacgaa
     4501 cttccagagg acttcatctg tcctctttgc aagcacggcg cggcggattt tgaaccgatt
     4561 aaataataac agtgcaataa ttaagaaatt ttgatatttt taagccgctt tttggaatag
     4621 gacgcagaat aattcttttc caaaagcggc ttttgtctta attatctaat gacaaaacag
     4681 gaatttaatg atataattgt aaaaagacgt caggatggtg atgttannnn nnnnnncggt
     4741 tgagactatg gcaaacttaa tcgtttttgt caaattcccc gaggacacaa cctcggaaat
     4801 taccgacaat tcgcagaaga taatgatgta ttataatgat acgtcaaaaa tgtatgtcgg
     4861 ctcggacatt gatttttcgt tcaaaaaata tatcaccgag atttcaagag gaaagctgaa
     4921 tgtcaataat attttccctc agcttgaggg agataagatt acacccctta ccctggcggc
     4981 ttcgcatgac aacagtaatg ataacagcgt tattcaggag attgttgctg cttttaattc
     5041 gggaaagata aagatgcctg aggataagct tgacaataaa tattccggcg ttgttgataa
     5101 tcttactgtt attattcagg gcaagtgccc gagcggctct gattttatgt ggccgcacaa
     5161 gagcgttacg gacgtttcga caaagataaa gaataaatat cagttcggaa actataatct
     5221 tattgattca tacagcgtta caggcaccgt agcggcttgt cagggtgtta ttacgcatga
     5281 atttttacac agtgtcggtt tgccggatct ctatcgcaga agcggaacgg acggaacacc
     5341 cgtcggtgtt tgggacatca tggcaagcaa cagcttcttt atgcagtatc cgctttccta
     5401 ccagcgatac aagcttggct ggataccgat gcagcagatc acccggagcg gaacatacac
     5461 gct
//
LOCUS       DAWBSW010000137         5388 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2787_length_5388_cov_2.454341, whole genome shotgun sequence.
ACCESSION   DAWBSW010000137 DAWBSW010000000
VERSION     DAWBSW010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2787_length_5388_cov_2.454341"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1432..1441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2921..2967
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3905..3959
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4225..4234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4639..4648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5020..5029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1364 a         1206 c         1022 g         1654 t
ORIGIN      
        1 tttacattcc gagcattgcc cgaaagcctt gccgaaatgc aagcgcttcc cgaggcaagc
       61 cttgacacac cgttcaaaac cgctgcactc accgtgcttg cactgtgtgc atacgccgcc
      121 gacaaaaaca tcggaacaga aatgctaaat tggctcagag gtccacgtcc gctgaacgga
      181 atggaaattt cctttttgaa cgaccgtttc cgtgacggaa agacctatct tccgtttact
      241 tattttgcgg gttcgacacc cgagaataac tacactcctt ccgagccata tacaatagtc
      301 gtcgaaagca atcatgtttc ggcagaggag cagggctata tgaagctctt tataccgtgc
      361 ggcggtgcag atgacccgcg tccgataaaa cttcgtcaac gcggaagcga cggaaaatgg
      421 ttcttatggg agcagtacct acttacaggt gtgcgtactc ccaaagcagc cgatccgtgg
      481 gcataagatc tgtaagatca ataaaaggca gtttttcgac aaaaacttaa accgcctccc
      541 ctcttcgacg tatgaacaca atcttaagtt catacattga aagctttaaa aaagcaacat
      601 aaaacataga agagaaatcc tgaaacgatg tcaagatttc tcttcttttt cttttccgat
      661 tttaaatata ttgaaaaaat ctttatgaaa attcacacct caaaaaaatc aaaagaaaaa
      721 caggcttctg ctaaaaagat tcatttcggc aaaagtctgt tcatgttttt taatacttta
      781 ttgtatagct gttctcacag gcaagggcaa atttaacgtt caaattgctg ccggcgacct
      841 taactccgag gttgagcaca tcggtgttgg tgtaaatcgc cttggtaaca tgacctgttg
      901 ctttatcgat tgtacactgg atttttccgt ttattccgtt gatttttacg ctttcaaact
      961 ttgttcccgg tacgttaatt tcctcaaagg ttgacgctga aacggtattg aatactcctg
     1021 catagcctgt tccctttttc gggcttgtaa gcttatcgtt ataaagaacg aatgtgattt
     1081 cataagtttt tcccgtgtcc ttaaacgagg tgctctttac catatccgag gtcaaatgcg
     1141 aagtatagct ttcaccgctt atagggaaat atgtcattat ctcatccttg cttgtaaagg
     1201 ttgccggttc atcggagcct ttaacaaact tttcaatcgc ccattttccg aggctgttga
     1261 tagccgacgg gagttggaga tattcgggta agctttgcat ttttgaataa tttcttgtga
     1321 ttgattttgc atttgattta atcttgtttg cagcagtatt ataaatattt accgcctcgg
     1381 cagtggtttt tgtcgctgac gaggttttgt tttcggtttt attgcttgat gnnnnnnnnn
     1441 nttcctccgc cgttgttcga cgaagcaaca atcgcaggcg ggttactgtc attagatgtg
     1501 tctgccgaaa cattctccga tgcaattccc gtacccgtgt cagccgcgaa ggagttaccg
     1561 ccctccgaga caccggcgtt atcggatact gcgattgatc cgcccgcctt tgccgtctca
     1621 atcttcgcat cgcatacctt tccgatgacg ctgtttgccg tagccgagac tgcaatgaca
     1681 cagatgaatg ccgttaaaga tgtcatccat gtttttttga tctccggcgt catcggagtt
     1741 ttttcttttt ttactttttc ctttttcata ctgtttctcc tttaaataac ggatttaata
     1801 ttgcttgact tttcagcgga tttataatac actattatta tatcaacagt ttgttgagtt
     1861 tacaatcatc agtttggctt atgattgcta ttagtcaaac atatattaaa tgaattggcg
     1921 gtttaattat gaatacaaaa aacaatcaac gtacaagact ttctaaaatg ctttttaaaa
     1981 acgcacttat ggatttgctc aaagagaaag gctcagttga aaagatatcc gttcgtgagc
     2041 tttgctctgt tgccgaactt aacaggtcaa ctttttatgc tcattacaac gagccgaagg
     2101 atttgctcaa tgaaatcgag aatgagctgt tagattccac cgagcagcat cttgaacgaa
     2161 tcggacaaga ggacgatatg ggagcgcaca gatatattct ttcattcctg aagtatatca
     2221 agtcaaacga caaacaattc cgtacccttc tcattgactc ggccgatccg gagtttcgtt
     2281 caaaattcat gcagcaatca atcattcagt tcattgtcaa ccttcaaatc gaattacccc
     2341 atgagcttga acaatatata ttctcatata ttctaaacgg cagcaccgga ataataatcc
     2401 aatggattcg ttcggactat tcggcagatg aaaacatgat tgttgatctg ctcttttcaa
     2461 tcaaccaaag cgctctcaca aatctaaacg tgaaaaaaag aaattagaac tcatgttttc
     2521 ctgcatgaac gctatgcttt tcaccgtttg gtaaaatcac ctcggcggta cagtttgacg
     2581 gtatttcaac ctcaaatttg tattcgccgt tttctttttt ccagccggat tttacttcgc
     2641 cgaaagcact cttgtagctt gctttggcaa atgaaaaatg tccgccgggc atgggcttga
     2701 ttacaaaacg gttttcgccg tcaatattta ttccgcacat tgacctgaaa agccattcgc
     2761 aaacagtacc cttggagtaa tggttaagag agcctatacc gccctgagcc tcggtcccct
     2821 cccatgattc ccatattgtt gtggcaccca tctttgtcat aaagagccag ccgggcattt
     2881 tttcgttttc aagaagcttg taggcataat caatgttaat nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa gattaaagta aattccacgc acaagctgag
     3001 cctgtctgtc ggtatcgagc gaatactgtt tcgatctcat aagagccctg tagctttctt
     3061 tgtttccgtt tgaaaaagcc ttataatcct ttgcgtcgtc cgctttcccg agcgcatcgg
     3121 ctatctctgc catacattcc ataacatagc ttgtataagc cgttgcggtt tcggggtgag
     3181 tgatcacaca ggttttccac gtctgcggat tgacatcctt cggctcagcc cactcgccgt
     3241 aggcctgtcc gaaattatta agaaattttc tgtttttgag cggaattgaa acatgctttg
     3301 cggtcggata cggcttgcca cagtgacttt tcataaactc ggcatatttt ttcattcccg
     3361 aatagtattt ttcgagtatt cttttgtcgc cgtactgctt ccaaaagcgg taaggaatca
     3421 aaactccggc atccgcccag cctacacagc cgtccattgc ggacatataa aaatccacac
     3481 cgcccggcgg aactatttgc ggaagctttc cgtttttctt ctgccaatcg taaatatcgg
     3541 caacgtattt ttcggaaaaa gcagcgtaat caaaaaggaa tgccgccgtt gtgaaaaata
     3601 tctgtgcgtc tcccgtccaa ccgtggcgct ctctcgtcgg gcaatccgtc ggtaaatcca
     3661 cactgttact tttcgttgac cattttgttg catcaacgag tttattcaaa agctcatttg
     3721 aactgtcaaa gaatcccgtt tcctccatgt ttgaatgaac ggctatggcc gtaaattcgg
     3781 catcggtgag atcaatatcc gtttctatct cgacatattt aaacccgaaa atcgcaaact
     3841 tcgttttgta attattctcg ccgtccttgc agatgtactc gatcttttga aggggcgttg
     3901 ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3961 tcgccgtttt tatcgagcat ttcgccgaac cgcattttta ttttttctcc ggcatttgca
     4021 ctcagagaaa atgaaatata tccggcaaga ttattcggaa attcaagaag agtttttccg
     4081 ctcggcgttt ttgagactgt cggtttaaat ctttccttct ctgcgatagg cacattgttt
     4141 gaacataccg gaacaacatt attatttgta accttggcaa atattccgta gctcggcgtc
     4201 attcgtgcgt cgacaatttc tccgnnnnnn nnnnggaaat cgaatttcac cgtcattgca
     4261 ccagttccat gttccgtccg taacgatttt cttaacctct ccgtcggcat agaatatctc
     4321 aagctcagca atgaatttcg tctgcgttcc gtactgattc gtaattcccc atgctccggt
     4381 tgaacctcga taccaaccgt cggcaagctg aatttcaaga gtgttcatgt gctttagcat
     4441 agaagtgatg tcatatgttt gatactggat tctcttgtta tagtcggtga ttcccggtgc
     4501 aagcggcatt gaaaatttct cgccgtttag cgttgcacag tatattccgc aagcggttat
     4561 acaaagtctc gctttcacaa cttctttttt aacagcaaat tttttcagga agcaatcgac
     4621 gggataacgc ttgcttttnn nnnnnnnntg tcaacggtat aattgcccgt tatccattct
     4681 cccgaccaat ttttgattcc gtattcaaaa aagcattctt cgctccactc gccttcgcag
     4741 tctttttcat cccacagctt aatcttaaaa ctaactcttt tacctctttt aaagactacg
     4801 gggactgtgc cgtgcattga gcttgattta accttgccgc tgtcccattc atcggttaca
     4861 attctgtatg cggtctgagt gattcccccc tcgcagttcc acattattcg agggttgtct
     4921 atatcaatac acaatggatt tttcaggtat tccgttttaa tgttaattgc tttcatcggc
     4981 tttttcctct tcctttctcg ccttaatctc ttcttgctcn nnnnnnnnna caggcccgat
     5041 gatagtgatt attgcatcgg taatatattt ttcgacattt ataaatgcaa gaattacaat
     5101 aagaattacg gcggtaattg tttcaagtcc tacgaaagag aacgtaatag cattttttgt
     5161 cgctgcattc tgaactgcgg caacggtttc tccgtttata attgaaggtg caatgtatcc
     5221 gctttttgac agaagtccgt taaaagctcc cgtaactata cccacacttg ttacggcaat
     5281 aatattgtaa atcgacattg caattccgtc gcagcggaag ttgtgtttcc attccaaatg
     5341 gtcaagaaca tcggcaaaaa gcgccataaa tacatatgag cacggaag
//
LOCUS       DAWBSW010000138         5349 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2820_length_5349_cov_2.340385, whole genome shotgun sequence.
ACCESSION   DAWBSW010000138 DAWBSW010000000
VERSION     DAWBSW010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2820_length_5349_cov_2.340385"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1170..1179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1796..1805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1475 a         1188 c         1057 g         1609 t
ORIGIN      
        1 aaggcttcca tgcccttttc gtactgctca tcaagatatg cgccgccgca aacgatcatt
       61 tcaaggttgc cgccgagatt gttgaggatc tcggagaaga tttttcttct cttatcaatt
      121 ccgagtttta aaaggaaatt actgatttta agtccttttt tgagcatttc ctctctgccg
      181 gatttcttcg ccgtgaacca aatcttttta taaatagtct caaccgcaag gggaaccgcc
      241 gaaaaattct gcggatgata tgtattcata tctttttgaa tatctttaag gctgcggcat
      301 ataaatcccc attcagtcag caaattgccc gagaaaagag caccgaccca tgaaaaagca
      361 tgatgaagcg ggagaaatcc gattgccggt ccgcccgtca taacacggca tgaagcaacg
      421 ctgtccgcgg caacattctc ctgcgagagc ataacgccct tgctcttgcc cgttgtgccc
      481 gaggtgtaaa cgaggaaggc aagatcgctc ggaagaacct cgtcctcaag ataactctca
      541 acgccgttct tgacctcatt atgaccctct tcaaccattg aatagaagtc ctctatttta
      601 atatattgct caattacaac accgtctgtt ttcttcattt tttcaacagt gtccttaaag
      661 tcctcgctat agtaaatggc attgcagccg ctgcggatca taatatcgat tatatcgtca
      721 tccggaagct tgggatccat cggaacagcg gttatttttc ctgtaataat cgaaaaatag
      781 caagcgatcc aatagtagct gttctcactg agaatagcta ccttttttcc tctgagaccg
      841 agcttgtaaa ggtactgtcc aagaccggtg aggtcatagt taacctgagc gaatgttttc
      901 atctcaacct cgccgtcaaa attatagaaa gtatactgag gcttgtctgc acctttttta
      961 ccgccaaaaa taactatgtc tttaaccgta ttcatgtccg gaaattcggc atattttatt
     1021 tttttcttca atttatccaa ctcctaacag cattatgttt tttattttag catataacaa
     1081 ggctaaaatc aacctatatt tttgtcaatg cgccttatat cattttacct ttaattgtgt
     1141 tttattgact ttaacaaaac aatgtgatan nnnnnnnnnc agtatcgtaa aatcaggagc
     1201 tttccaaaaa atggaaaagc agaaaaaaac agataaaaga tttctcttaa ttttcattgc
     1261 aatgtttgta ttctttttcg gccttgctta tatgcttcct tacaccggtg atgactgggc
     1321 atggggcgga cagataggaa tagaccgact tcatgagcat tttagaaact ataacggccg
     1381 atatctcgga aacctgctcg taattgcgtt gacgagatcg aatcttctca agtcctttgt
     1441 tatgtccagt actgtaacgg gcttggtttt tttaattcat caatctgtta aacataagag
     1501 catagcaaca cttttgcttt cgctgacagg tctttttgtt atgccgagat atattttcag
     1561 acaaacaatt gcatggacct ccggttttac aaactatgtc gcgccgactt ttttggtaat
     1621 cgcatatatt tgcatcataa aatgcatttt tgacggcaag ccgaaattca ggaaaggaac
     1681 ctttgctttg accttcctcc ttggcctgag ttctgccctt ttcatggaaa atattacatt
     1741 atatcagatt gcaattgaca ttataattat cgttggaacg cttgtgggct ataagnnnnn
     1801 nnnnnatatt tgctcctgtt atcagccact ttttgggttc tgtcgccggc tgtgcaataa
     1861 tgtttaccaa cggagcatat ctgaatatag ccaattcaaa agacgattac agaaccatgg
     1921 aaacaacttc aaccggtatt ctatcgcgca ttaaagaaaa tctatttgac gtcataagaa
     1981 aagaccttgc gcttaacaat gttgttctta acctttttat agcacttgta tgtattgcgc
     2041 ttgtcatatc ttttttcaaa aaggataaag attgcaaact tttcaaaaag cttttcatac
     2101 gttttaatct gtttattgtt atatcttacg ccgcttattc tctatgccgt gtgatttatc
     2161 cgaactggaa tatcttcctt aattatacaa agtattttga gagtgttttc gttttgatct
     2221 tcggcattgc ccttataatg ctcagccttt tatgcgtaga cgaacccggt gttaagctca
     2281 gactcagctt ctacatagtc agcattgccg ttttgacggc tccgctgttc gttgtcacac
     2341 cgataagaag ccgatgcttc ttctgtggat atatgttctt tgtcctgttc ctttgtgaag
     2401 ccttcggcta tgtttttaac gaaaagcatt ccttgattaa aaacggaaat ttgtcgcgta
     2461 tcttacttgc aatttgcatt tgcggcgtaa ttttctatac aactctttac ggttacattt
     2521 ttgttgcgga aagagatcgc aacacctata ttcaggagca gatagcagaa ggaagaagta
     2581 ccgttgaact tcccgaactt ccgtatcagg gatatctttg ggattccact ccgaatatca
     2641 actccatgtg gggcgaccgc tataaggcat acaaaggaat tgataaaaac agcaaactcg
     2701 aaatcgtcag atattccgac tggaaagaat caaccgaata acagcaaatc ccgaacgttc
     2761 attaccgagc gttcgggatt tttttcaatt caatattccg aggctgacag acaatgctct
     2821 gttcactctt gtcatgtccc tttcgccgag gttgcccatg cgctctttga gcctttgctt
     2881 gtcaagagtc ctgacctgct caagcagaac tattgaatcc ttagagaggc cgcagtcctg
     2941 agcgtccacc tgaatatgag tcggcagttg agttttgaag cgctgacttg ttattgcggc
     3001 ggcgatgacc gtcgggctgt atttgttgcc gatatcgttt tgaactatca gcacaggacg
     3061 cataccgccc tgttcagagc ctacgaccgg gctgagatct gcataataaa tatctccccg
     3121 ttttattgtc atttttatca ctctccgaga ttttgattgt cataattata tttgccaatc
     3181 atttgacgga ataatcatct ttttcgggga tcagaaatga tatccggcat tatcattata
     3241 ttcggtttga caaattacgt taatttctat aaaatataat tgaaaaattt atacatctcg
     3301 aatatacggt atcgccttag aatagccgca tattaagccg tcaattttca aactataatc
     3361 ggaggtgttc aaaatggaga aaaagaatgt cggaaagaaa aaaacgatat acattatttt
     3421 gaccgctttt gttgtttttg tcgccgtgat atgtgcattt gcactccccc ataaagctaa
     3481 gctcgacacc gagacggcaa gctatattga aaagcagata attgcccggg aatcgccaac
     3541 ggcatatcat gaagcaaatg acttttccgc tgtctcatat aaggttctca gtacaaaaag
     3601 aaaaggtgat acaacagaaa tatatatggt cgcactctat gaaaattttg tttacaaaaa
     3661 cggcttgagt gtaatttcag gaaactcggg tccgctggtt ttgactatgc aaaagacaga
     3721 cagcggatat aacctgaaag aatattggac gccgagcatg ggtgcggatt acagtcggag
     3781 cataaaagaa agatttccgc tgagcttgca gtataacgct atcggcagca gcaaatacgc
     3841 cgacgagctg aaaaaagaaa atcttctaaa ggcttcggaa catttcggag taagcgttga
     3901 cgaccttgcg acagctccgc aaggcgaaat aatgagcacc actgccgtaa acgttgccgg
     3961 aagctaaagc gataaatcgc acagtttcag ttcaagcctg actcagataa aaaacgatgt
     4021 taaattacag cattgcagac gtagcgaccg ccgaagtctc cgtacccggt ggtgcaaggt
     4081 ttgttaatga ttgacggtta cagcgttatt aagcctcctc ttaaatgatt taaggtgagg
     4141 cttgaaaaat tctttcggct tcaaaaagag attattaaag cttcagcggc tgcgccataa
     4201 ccggcacagc cgctttttta ttggaatata taccttatct tatccgcttc aatctcgcga
     4261 atttttccgt cctttttgtt tgatattgcc gtgcactccg tttcgggcgt gcgatatttg
     4321 agaatataac tgtcaccgct cattcttgca gattccggca cggtgtagca cacggttttc
     4381 agcaccgaat aaagtccgat aaacggacaa aaccctttcg cctgctccgt cgtgtagtca
     4441 atctccatgc cgtcatagcc gagccgacag ccgctgtcgt caacgctgag agtcattccc
     4501 ttaatcggtg aaagcatttc aaccgtcatt ctccccgttt catcataatt tatccttgcc
     4561 gaacagtcaa taccgttata gcttgcgctg acggtgcaat tcggccttga cggggtgacg
     4621 ttttctttct taattctttt tccgcacgag ggcaaaaaaa tcataataaa aatgagaagt
     4681 attgcaagac tctttttcaa aattatcccc gattattcaa aatttgaaag aacctccgct
     4741 atctcgccga tcatttttgt cggggtaact ccgtgcatgg agtatttttt ttctatgtta
     4801 tctccgcaaa gtccgtgcac atacacgccg gcaatcgttg ctgactgcaa gctcatagac
     4861 tgacaaacaa aagaggaaat tatacctgcc aaaacatcac ccgaaccgcc ggttgccatt
     4921 cctgcattac cggtacggtt tatgtacacg cttcccgacg gtcctgcaac aacggtattt
     4981 gagcccttga ggacgagcac acagccgtac tgagttgcaa aattccttgc agccttaacc
     5041 ctgttgcttt gaatttcttc aatgctcaga cccgtcaatc ttgccatctc tcccgggtgc
     5101 ggagtcaaaa tcaacggagc agaggtttct ttcaatatat ttatattaaa cgaaaccgca
     5161 tttattccgt ctgcatcgag aataatcggt ttttttacgt ttgcaagaat cccgttgaca
     5221 aggcgcaccg tgtcctcgca aagtccgagt ccgcagccga gaagtaccgc cgttgattta
     5281 tcggcaagct cgatgatgcg ctgcaaatta tccgccttaa gaaaaccgtc agccgttgag
     5341 ggcatagga
//
LOCUS       DAWBSW010000139         5345 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2825_length_5345_cov_2.961437, whole genome shotgun sequence.
ACCESSION   DAWBSW010000139 DAWBSW010000000
VERSION     DAWBSW010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2825_length_5345_cov_2.961437"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1361 a         1283 c         1120 g         1581 t
ORIGIN      
        1 gcctcttctt ggcacttcgc ttccctcctt catagtaatg atatcatagg tactcaagtg
       61 acaaaaccct gtggcgccaa tggagaagca catacataat atatatatac actctcacat
      121 tgatacgcag cgtaccttac cctgcgcagc ctgcaagaag attctgtctt tcaaactttc
      181 cttttaaaaa gaggaattac tttttagcag ccttcggacg cttagcaccg tactttgaac
      241 gggactgcat tcttccgtta acaccctgag tatcgagtgt gccgcggatg atgtggtaac
      301 gaacaccggg aaggtcctta acacgaccgc cacgaataag aacaacgctg tgctcctgaa
      361 ggttatgacc tacaccgggg atatacgaag aaacttcgat accgtttgta agacgaactc
      421 tggcgatctt acgaagagcc gagttaggct ttttaggtgt agcagtctta actgctgtgc
      481 aaacgccgcg cttctgaggt gaattctggt cggtaacaat gttcttctta gaattgagac
      541 ccttgagaag agcaggagcc tttgctttct tttcgatagc ctgtctgccg tttctaacta
      601 actgattaaa ggtaggcaaa aactacatct cctttcttaa aaattttgaa tattgacaaa
      661 tcatatgccc atggggacac ataataaatc acattatcaa tctttggtat tctatcactt
      721 atgcatatat attgtcaagt gtttttgtaa aaatattcta aatttttgaa aaatcgggga
      781 ataaatcaaa atcaatgcct attttagcga tttatgttgc ttagatcgca cgaaatgtgc
      841 cttttccgaa actttccgta aggtgcgatg gccgctgcac cttggaaaat ttcttcaatc
      901 agcttttctt tatcccctcc gtcgccatag acgacacctt ctcgctgcgg ctcggtcacg
      961 ctccagctct gaacgtccgc cggacgttca ttcactaccg tcgcgccgct ttgctacctt
     1021 gcaacaaggg gaggcatgaa aaatttcttc tgacttcgga agtaccgaaa tattttactt
     1081 ataatacacc gttctgccga taaagattct tacgaagaga tgaccgatcg tttcacaggc
     1141 aagctcaata aagccgagga aattgacctt atcgacaagc tttgcgccgt ccttatcccc
     1201 tgcttttctt aaagcagttg aatcgcaggc agaaaaagca ttgtaccagc gctgggttct
     1261 tgtctgatat gtcgaagtat cattctcatc aaggtcgata attctgtaac cgtactgtga
     1321 tgaataggca tcgccgttat agcgcgtact cagtgaattg gttatattta tgcccttgta
     1381 tttaacgctg aagctgttgg agtggtcgtg acccgtgaac attgcgagta catctcccct
     1441 ctcaaccaat gcgtcaaact gtccgtggtt ataatagccg caggacggcg attcgtggag
     1501 cgtgccgtga ttctcgactt cgggatcgaa agcgtagaat tcatcgtctg tatagaactt
     1561 attatacaca tatgcgccct tatgcttcat ctttttgagt ccgtcataaa cctcagggac
     1621 aataatatgc tgaaagacaa atgaattgat cttaccgccg ttctgctttt cgatctctgc
     1681 ggaggtctgc ttgtaccatt caacctggtc agcctgtacg tttgagtaat gaccctcact
     1741 atcataatcg cccgaatcga atacccaaag gttaaacttc attttgccgt cgatggacga
     1801 atatagcgga acgttgtatg tgccgcagcc ggaaagcatt tcgccatcgt caatcgaaat
     1861 cgagcaagga tattcattgt aaagagccat gagatcctct cttgaaataa cgccgttttc
     1921 ggagtcatga ttaccgaagc aaaccgcgac gggaatatct ctcgactgga aaacgctcat
     1981 gatctgctcg ataaaccatt tggtttcctc cacggtgtcg cacttgcaga tatcgcccgt
     2041 gagcattatc aaatcaggct tttcacggtc gcaggcattg gcaataagcc agattgaagc
     2101 tttaacgtta tccttgctga ggtgcgtatc ggtaacgtgc attattctga agctgccgtc
     2161 ctcattgaag taaagaggtg cggttgtcaa tgacttgtcc gcggcaaagc cggttgaagc
     2221 cattgcgaat gtcaaaacga ataccattat aaatgataca agaatgttta attttttcat
     2281 agtaaatacc tttctttttt atacggtgag ttgtgctgaa gtcggaagga gtttaagtct
     2341 gataagacct tactccctca gtcggcttcg ccgccagctc ccccaaggga ggagcccacg
     2401 aattttcctc tcaccttgag attttttcaa acttaattgt ttgtaaaacc tcagttttat
     2461 ttgtaagggc gatcatcggt cgtccgcttt tgttttttag aagactttgg cggacgtgca
     2521 atgcacgccc ctactgagtc aaagttagtt ttatttaaac taaaattttg ataaaggcta
     2581 aatgttgtgt actaatcccg cagcctccct cttgagggaa gccttggtta aattcttctt
     2641 aactgtaatt tcttactccg taccgataat ccaacaaatc aatccgcatt gacgagccat
     2701 tcatgctcct tggagtaaag acgtgtggtc ttgtaccacg ggtcgccgtc aatatggcgg
     2761 atcatccata cataatacat tgtgtatccc ggagccgtaa cctgctcgtg gtcgttgccg
     2821 tcggttatcg tgcaatatgc gccgtcggtg ctcttgaaaa cgttgtctcc gttgaagcat
     2881 gcgccgaagc cctgcggcgg atcgaactga tagtaataaa cctcgggctg cggatgatgg
     2941 tgcggcggat agctcgacca tctgcccggc tggttgaaca cctcgcccat taccatgttg
     3001 gaataggggg cattgtcata gtcgaacatc gttgagcaga tacggtagcc tgtgccctcc
     3061 cactgacctt tgccgaaatg ctgataaagg caatcctcag gcttatagaa aacaggctca
     3121 aactctctct cgttatcggt ctgctgaaca aggaactcgc tgtcctcgca agcctcaatg
     3181 cttatcttca cacccttgca tacatgcagg caatacggtg ttttctcgaa cggattcttt
     3241 cttttcatgt tctctgttct gtcattccag cttatattca tgttgccctt gaggatgagc
     3301 actgccgtct cgttctcggc actgaaaatt tcgtacttct ccccttttgc aagctttttt
     3361 acccggatat tcatgtgcat ttcgggattt ttgccgttca tttcgcagag aatcttttcg
     3421 ttattctcgt tgaatgcagg attttcgagc atatataatt cctccttgga aaatgttaga
     3481 tttacggtta tattctatca ttctgcaata ataatttcaa tagcagtttg caaaaatgtt
     3541 caaaacatat atcattgttg tatgccttga cattgcaaaa aaacggactt ataattgtat
     3601 atcatcagca aaagaaggtg tttttaatgg tcattgatgc acactgtcac atctacccca
     3661 ataagattgc cgacaaggcg gcgcacagta cgggacgctt ctattccctg cccacaacct
     3721 acgacggaaa ggtttccacc ctgctcgaaa acggagcgaa ggcaggtatt gataaattca
     3781 tcgtacagtc ggtcgcaacg acgccgaagc aggtcagctc tataaataaa ttcattgccg
     3841 ccgaggtagc cgctcacccc gacaagctct acggtctcgg tacgctccac cccgacagcg
     3901 aggatatcag gaccgacgtt gaggagcttg tttctctcgg tctcggcgga gttaagcttc
     3961 accccgacat tcagcagttc aagctcgacg attacagatg ccttaaaata tatgagcttt
     4021 gcgaagaatt caagctcccc gttcttcttc acacaggcga cagcagatat gattactcaa
     4081 acccgaacag gctcattccg atactggata tttacgacaa aatggttgtc gtcggcgctc
     4141 atttcggcgg ctggtcgatg tgggatgagg cgtcaaagct tcttgccgac cgtgagaatt
     4201 tttatgttga ctgctcatcg tcgttctatg ctctctccga cgctaaaatc cgtgagatca
     4261 tcgcacgcta cggcgttgac cgtgtccttt tcggaacgga ctacccgatg tggagcgttg
     4321 aaacagacct gaagcgtttc tatactctcg gctacaacaa agaggattcg gacaagattc
     4381 tttatcaaaa cgccgttaag gtctacggct tgaagctgtg attggcattt tcctatattt
     4441 tattttctgc ttataaataa tgtgggcatc gtacactgca aatatggact caatccatat
     4501 ttgcagagca cgatgcccac tgcttcatta aagttgactt taatcaataa aatctcagaa
     4561 ttctatttta tagaaaaacg gtttgatatc cgcataaatg cggctataat tttcacgttc
     4621 agcggttttt ctgtcaagaa ttttatagat gtcggacatg tattttctga gttcctcggg
     4681 catggtattt acccctgtca aaatcacctc atctgcgtca cattcactgc ttaacaaatc
     4741 ccaaaaagcg tctaaattag caccgtacca ttcaggaaaa tcaaatgctg tcctaattcg
     4801 ttcatgaagc tctccgagat acttacaatc cgttaaatcc aatgtaattg ttttcttttc
     4861 ttccattgcc tgctcctata tttaaacgag ccgttgcaca tttcgcttcg gctcgcttta
     4921 ttattctgtt tacctccaaa ggtcgtccgc tctgacgatc tccctgtcgt ctctgccgcc
     4981 gttgagctgc gggaagtacg acgcaaatct gtatctcggg agcttgtcga attcaagctt
     5041 tatgagcgga taatttataa tcggatcggg atttttttcg ggcaaatcgt atataaaaat
     5101 tctgtcgccc tttttctcga attttatcgg agtgccgtcc atgaaggaaa tcttcttagg
     5161 ctcgtccata aatccggcaa gagccatttt tccgttgcac ggcgcccaaa tcttgttcca
     5221 catataaatc gttgtgccct ttgacgtacc ccttccgacg ccgttgagct ggaatttgtt
     5281 gccctcgccc ttgttgagaa taccgtaaac cacttcgccg ttgaccttga accactcacc
     5341 tacct
//
LOCUS       DAWBSW010000140         5306 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2843_length_5306_cov_2.328318, whole genome shotgun sequence.
ACCESSION   DAWBSW010000140 DAWBSW010000000
VERSION     DAWBSW010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_5306_cov_2.328318"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1351..1360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1494 a         1006 c         1161 g         1635 t
ORIGIN      
        1 caacagccga gagtattctt gcattcatgc ccatggctgc catgggattg gtcatagcgc
       61 tcggcggcgt tttgcttgcg ggcaacgatg ttcaaaaaag ctacgaatat ttcttcctta
      121 ttctcggtgc gactgttttg gtatgcggaa ttatcggttt gtttacactc aaggatgcac
      181 cgaaaaccgt tgaaaactcg ggcaattttt ggggtgacct tatttacggt cttaaaccgt
      241 ccgtcattaa agaaaattca aagctttatc ttacattgct tgcggtctgc gcttattcaa
      301 ttgcggtgca ggttttcttc ccgtacctta ttatctatct tcagcacagc cccgacggat
      361 ttttcagcgg agaatcgctt aaaattacac cgacgctgat tatagttgcg gctgtttgtc
      421 tcattgcgct gattgtcgga attatattag tcggaaagct tgccgataaa tacggtaaaa
      481 ataagcttgt tataccgatg ctggttttct ttgttgcagg gcttgtaatt ctttatttcg
      541 cacatgatat aaagcttctt ctcatcggcg ttgtacccac ggcggtaggt tattgcttgc
      601 tcggaatcat gctcaatgcg gctgtcaggg attttacacc cgagagcaaa acaggtcttt
      661 tccaaggtgt gagaatggta tttgccgtaa tgattcccat ggttgtcggt ccttatgtcg
      721 gcaaaaaagc gatattcagc tcaggcgttt actataccga tgactacggc gtttcgcaga
      781 ttgttcccgc ttcgtcaatg ttcctttacg cggcgatcgt ttgcgcagtt tcccttgttc
      841 ctattgtaat tattctttgc aagggcggtt ttgccgtgtc ggaaaacaag aataaataat
      901 gctttaaata agatccgtat tgacgggtct tattttttga ccgcaaaagt aataaaaacg
      961 gattacatat ttttgtatat taggctgatt ttcagacgat taaagcataa ttatattgac
     1021 aatttgttgt tttgtagtat aatacaatag cgtatttgca cagtggaata atgtgcctta
     1081 gcaggtttat ctttaggtga ttttcaaaca atagtcaaga aggttttacg atgatatctg
     1141 ttgttcttgc cgcatataag ggcgaaaaat atatcggagc tcaggtcgag tcgataattt
     1201 ctcagctcgg cgaaaatgat gagctgatcg tttcagacga tttcccgacg ggagatacaa
     1261 gaaaagccat tgccgatttg attgaaaatg acaagcgaat catctatctc aacggacaag
     1321 caattccgtg acagcatctt tcagaccgtc nnnnnnnnnn gatttggttc tccacaatgc
     1381 gtatattacg gacggcgagc ttcacaaaac aggtgagacc gcctttgaaa tcaataatac
     1441 aaaaaaaggt tttttcagaa atataattaa aaactcatat cagggctgct gcatggcttt
     1501 tcgctcggag atgaagaaat atattcttcc gtttccgaaa aagattccga tgcacgatca
     1561 atggatcggc ttgattgcag agaagcacgg cagaatcggt ttgataaacg agcctttgat
     1621 tttatatcgt cgccacggcg gcaatgtcac gggtaacgga agcaatttta ttacaaagtt
     1681 caaatggaga gcggatatta ttttgtccgt catcggacgc tgaacgttac cgaaaaagga
     1741 aagtaggtta gtgtatgaac aatatggtct cgggaagcat tgttactttt aacaacatct
     1801 ccaccataga aaaaacgctt gaaacaattt ttactcaaac taaggatatt gattttaaac
     1861 tttacattgt tgataacggc tcaagtgacg gaacgcctga atttgttgaa aataattatc
     1921 ctcaggtttc ggtcattcga aacgaaaaaa atgtcggctt cggcgcggga cataatataa
     1981 ttattaactc ggttgaatcg agctatcatg cgattatcaa tcccgacatc gtgctgaaag
     2041 aaaatgtaat taaaaaaatg gttgactaca tggaaaaaaa tcaggatatc ggtctgcttt
     2101 ccccgaggat ttgtttcccc gacggacggg atcagatact cggcaaacgc aatccgaggc
     2161 ttaaatatct cgttgcaagc aggcttagag gcgatgagcc gggaaagctt ctcagggagt
     2221 atgctatgct tgattgtgac ctttccaagc cgattcaaat tgaaaacgca acgggctgtt
     2281 ttatgcttct gagaacggaa gtgcttaaaa aaatcggcgg ctttgacgac ggttatttca
     2341 tgtattttga ggacgccgac cttacaaggc gtgtcaacga ggtttcaaag tgcgtttact
     2401 atcccgatgc gacagttaat catgtttggg gcagagagag caaaagaaat ttgaagctga
     2461 tgtgcataca cattgattcg atgtgcaggt atttctgtaa gtggaaaacg atatgaggtg
     2521 atacaatgga tgaaaatacc aagaaaattc cattaaagca atatgcaaaa aatgcataca
     2581 aatacagata cttgatgcag gaaatcgttc gcaagaacgt caagctgcaa tacagaaatt
     2641 ccttcctcgg aattttttgg acaatgctgc agccgtttct tacgatgctt gttcttgttt
     2701 ttatctttac aaaggttttc ggaaaagcga gcgacggtat tgtttgctat cccgtttatc
     2761 ttcttacggg acgtcttttg tatgagttct atactcaatc aaccaacaga gcaatgagat
     2821 caataagaaa cagtgcttcc gtgctgaaaa aggtttatgt accaaagtat atttacccga
     2881 tttcaaatgt attttctacg tttataacgt ttcttatttc gttgcttgtt ttggtttttg
     2941 ttatggctta tttccttatc aaaaatcagt atacgcacgc atatcccgat ctgcatcttt
     3001 cgtggtatat ttttctttct tttgtgccga ttatatttct ttgcatattc agcacaggcg
     3061 tcggccttat actgtctgtt cttaacgtat attttaagga tatcgaatat atttacagcg
     3121 ttattacgct aatgcttttc tatgttacgc ctattatgta cagtctcaaa accctgcaca
     3181 agagcggtgt taatgcaatt gttatgaaag ttctgatgct taatccgctt tacagtattc
     3241 tcgaaatgtt ccgctcatgt gttctttact gttcaatttg gaactggaac catttctttt
     3301 atgcgttggg cgtttcgatc gtcacgcttg caatcggact tatcgtcttt tacaagcatc
     3361 aggatagatt tatcctttat atctgagagg tgacattatt tttatgagtc agccggctat
     3421 tgaagtaaaa aatgtcagca tacttttcaa tatgaataaa gaaaaggttg acaatttaaa
     3481 ggaatatttt ttaaaacttg taaagcatca gcttatgttc acggagtttt gggcattaag
     3541 ggacatttct ttcactgtag aaaagggtga aaggctcgga gtgctcggat tcaacggtgc
     3601 cggcaagagt accttgctca aaaccgttgc cggtgttttg aagccgacaa agggctcggt
     3661 taaggttcac ggagttatag ctccgctgct tgaattgggc gcaggctttg acagcaacta
     3721 ttccggcaaa gagaatatct tcctttacgg tgcaacaatg ggctattcgc ggaagtatat
     3781 cgaagatagg tatgatgaaa ttgttgaatt ctcggagcta aaagatttta tagatgttcc
     3841 cgtaaagaac tattcctcgg gtatgagagc aagactcggt tttgcgatcg caacggctgt
     3901 tgagcctgag gttcttatcc ttgacgaggt tctttccgtc ggtgacgcaa agttccgtca
     3961 aaagagtgag aacaaagtca ggagtatgtt tgacaagggc gttacggttc tctttgtatc
     4021 tcataatacc gagcaggtca gaagactttg cgacaaagcg attattcttc aaaaaggtca
     4081 gattatcgct tcgggtgagg taaacgaggt ctgcgacatc tacgacacta tggttaataa
     4141 aaaatgattt ttcggggata gggaaaaaac ggagagtcat atgaagaata tattttatac
     4201 tttttatgcg ataatgttcg caatttttcg catttttcct gtaaaactga ataaaattgt
     4261 tttcctttct ccccataacg agaattttaa cgacagcctc ggagcggtca tggatgaggt
     4321 cattcgacgt gacgagttca gagtgattat gatttcgggc agagatctta aatttaaccg
     4381 ctcaaggccg tttcacagca taggcaggat aatcggcttt ttcaccggaa aggcttttca
     4441 ccttgccacg gcgaaatatg tttttttaaa tgataatttt atgccgatgg ggaagcttca
     4501 tttcaacaaa aaagctgtta taactcagct ttggcatgcc gagggagctt ttaaaaaatt
     4561 cggtcttgat atagagcagt ccgaaaaagt cagagcgctt gaaaaggcag gcaacgcaaa
     4621 attgacctat gtcgtttgct cctcggaaga tgttgctccc atttatgccg gtgcattcgg
     4681 cgtatctgat tataaggttc tgcctctcgg atctgcgaga tcggattatt tcttcagaga
     4741 ttttaatctt gaccgtatgc gagccgattt tgacaagaga tatcccgaat gtaagggcaa
     4801 aaagcttatt ctttacgctc ctacattcag agataatccg gcggacgaca gcagaattct
     4861 tgcaaacttc gatattcaac gaaaggttcg gcgatgagta ttctctcctc atacgtttgc
     4921 acccgcaggt tcatgccgat gttgaattcc acggagccgt tgacgtttgt aaatatgaaa
     4981 acacgcttga gcttatgaag ctttgcgaca ttcttataac cgattattcg tcgatctgta
     5041 tggattttgc aattcagaac aagcccgttt atttctacgc ttttgatttg gaacgctacg
     5101 aaaaggaaag agctttttac tttgactatg aaagctacgt tcccggacct gttgcaaagg
     5161 attttcaaac tctccttaat ctcctgaaca gcaatgtttc cgagacctac agattgagaa
     5221 gaaatgattt caactacgct aatttcggta cgctcgacgg aaaagctgcc gaaagaattg
     5281 ttgataaaat aattaacgac aaataa
//
LOCUS       DAWBSW010000141         5287 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2854_length_5287_cov_2.504014, whole genome shotgun sequence.
ACCESSION   DAWBSW010000141 DAWBSW010000000
VERSION     DAWBSW010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2854_length_5287_cov_2.504014"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1631..1640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3713..3722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1645 a         1091 c         1221 g         1310 t
ORIGIN      
        1 gtgtaatata cttgtaaagg aagttataca tattttgtct cctttattct ttgcgaccct
       61 tttctttttt aggaggaacg gggtcataac ctcccttaaa taattgattg cacctaagca
      121 atctgccgag agccataagt gagcctttaa atgctccgta aacttcaatc gcttcaacgg
      181 cataatggga acaggtcgga taatacctgc acatgggcgg cagatgaggc gaaatgtgcc
      241 tttggtaaaa cttgatgcat ctgatgagaa agcttctcat atctttttca cgcccgcttt
      301 tgaaagttgc ttcgacataa ttgccgaaat atccgtgctt ttgagatgtt tggttctgga
      361 tctggcaaca aagacaaagt catatccgtt ctttaaatac ggaagattat ctctgaatgc
      421 cgctctgatg atccttcttg cgcggtttct ctcaaccgca ttgccgatct tcttacttgt
      481 ggtaataccc atacgacaaa ctcccgcgcg gttattcttt aaaacataag taaccaacgc
      541 aggatcagaa aatgattttc ctctgccgta agctcgtcgg aaatcgctat tttgctttaa
      601 aacaatataa ttcttctcgg gcaaaaacat caaatccgtt caacggtggt ctaccgtaaa
      661 atccaagtac gtttaatcag tcaggcaatc gcctgccaat caaacaactc agtaagtaag
      721 ctgctttctg ccctttgcac gacgacgtgc aagaaccttg cggccgtttc tgtcagacat
      781 tctcttgcgg aaaccgtgtt ccatctttct gtggcgcttc ttgggctggt aagttctctt
      841 catctttctg acctccgttt caatactgta ctttgttatt ctgagtgtca acaatgcaat
      901 aatgcatatg cactcacgtt gagatattat ataataaaat tcgccctgtg tcaatcatta
      961 ttttgtaaat aaaagattta catttaaaaa attattatta ttttacgtta taattgttta
     1021 aattactaat tgcatttaat catttcgtat gctatactat ttcggtaaaa atcaaacagg
     1081 agatgtaaaa aacacatgga ttcattcgca gaactttggg aaatggtaaa agaggaatgt
     1141 aaaaatcagg tttccgaatc catatataat gtttggttca aggacatgga gcttgtaagc
     1201 tttgacgata acaaggtcgt tatcgctctt tctgacttta agcgtaaaat tgttgaaagc
     1261 agatccatgg ataagctgga atcttccttc aaaaacgtta tcggttttga tattcaaatc
     1321 attcttgttg atgttgaaaa agcggtaccc gttaaggctg aggaaaccga agacgctctc
     1381 acggacgagg atacgtttga ttccttcatt gtaggcgcat ctaacaaatt tgcttatgcc
     1441 gcagcaaacg ccgttgccaa tgacccgggc ggaaaatata atcccctttt cattcacggc
     1501 aactcgggac tcggcaagac tcacctgctc aatgctatcg ctcatcaagt taagcttacc
     1561 caccctgaag caaatattgt atatacacga ggagagagct ttacaaacga gcttgtcaaa
     1621 tatatcggac nnnnnnnnnn cacgataaat acagaaacgc cgatatactt cttgttgatg
     1681 acgttcagtt tatcgcaggt aaggactcaa ctcaggagga gtttttccac acctttaacg
     1741 cgctctatca ggcaggcaat cagatcgttc tgacatcaga tagaccgccg aaggaaatag
     1801 ctctccttga ggatcgactt cgcaatcgct ttgaatgggg ccttatggct gatattcagt
     1861 ctcccgatct tgaaacaaga atggctatta ttaaaagaaa agccgaggtt ctcaattttg
     1921 accttccgga cgatatcgtt caatacatcg ccgagaagct taaaaacaac attcgtcagc
     1981 ttgagggcgc agtcaagaaa atgcaggcat tcgttactat gcagggtatg cccgtcaata
     2041 taatgacagc tcaaacggca attaaggata tcatcgtcga taacagtccg actccggtta
     2101 cgattaacaa gattgttaca gaggttgcga gaacctacgg agcggacagc gccgatattt
     2161 attccaaaaa gcagaatgct caaacgaccg aaatgcgtca gatggcaatg tatatagtca
     2221 gagaaatgac cggactttca acaaagctta tcggacgtga attcaacaga gatcattcaa
     2281 cggttgttta cagtcttgat aaatttacaa agcgctacca tgaggacagc aagctaagaa
     2341 gcgttgttga caatattata aagaacatcc gcgacggacg ttgatcggag gaaaatatgg
     2401 cttttggtat ttttaaaaag tttaatttag gtctgaaaaa gaccagagaa accatgtcgg
     2461 gtgctatcga cgccgctatc agcgaaaatg aggatatcac agatcagctc tatgacgatc
     2521 ttgaagaaat tctcatcatg ggcgatgtcg gaatgaatac ctcggctgag atttgcgaga
     2581 agctcagaga atatgttgca aagcaccatc tcaaaaaatc ctctcaggtc aagggcgcaa
     2641 tcaaggagat cgtttcggaa atgcttgagg gcggcgaaga tatgggtctt gtcaccatgc
     2701 cgtcaatcat tcttgttata ggcgttaacg gcgtcggaaa gacaacgact atcggtaaaa
     2761 tggcggcgat gtataaggct cagggcaaaa aggtcattct cggcgcagca gatacattcc
     2821 gtgccgccgc aatcgaccag cttgaggaat gggctaaccg tgccggagtt gatatagtca
     2881 agcaccatga aggtgcagat cccgccgcgg ttgtgtttga cacgattcag gcaggtattg
     2941 caagagacgc agacatcatt atctgcgaca cggcaggacg tttgcacaat aaaaagaacc
     3001 tcatggacga gcttgcaaaa atttacagaa ttattgataa ggaactcccc tataacgacc
     3061 gtgaaagcct gcttgttctt gacgcaacaa caggtcagaa cgcagtcaac caggcaagag
     3121 acttcaaaaa tgtttgcgaa atcaccggaa tagttcttac caagcttgac ggaaccgcaa
     3181 aaggcggagt tgttcttgca ataaaaaatg accttaaggt tccggttaag tttgtcggcg
     3241 tcggcgaaaa gatagatgac cttcagccgt tcaatcccaa ggcttttgcc gacggactct
     3301 ttgacaatga agtaaaacat gacgaggaaa acatgttcat ggacacagaa tcggaagaaa
     3361 agccgtcgga ggaaaaagcg gaatgaagct tctgattgcg acacataata acaaaaagaa
     3421 gatcgaattg cagcgcatat tctcccctct cggaattgaa gttgtcaccg ccgaggatat
     3481 cggatgtgat cttcgtgacg ttgaggaaac cggaactacg tttgaagaaa acgcaaagct
     3541 caaggccgtt gcgggctgtg aggacagcgg aattgcgtgt atcgccgacg attcgggttt
     3601 atgcgttgat tatcttaacg gtgagccggg tgtctattcg gcacgctatt cgggtataca
     3661 cggcgacgac gaagcgaaca ttgacaagct tctgaaaaat ctcgaaaacg ttnnnnnnnn
     3721 nnaacggcaa gatttgtctg cgccgcatgc tgcgttttcc ccgacggtaa aataataacc
     3781 gtccgaggcg aatgtgaggg agaaattctc atggaaagac acgggaacgg cggattcgga
     3841 tatgatccga tttttatggc aaacggcaag cccttcggcg agataacatc gcaggaaaaa
     3901 gattcaatga gccacagagg aaaagcgctt agacttatgt cagagcagct taaaaatatt
     3961 attacggagt aaaatatgga agaaaaaatg acaagcaaaa gacgtgcgga attaagaggc
     4021 atggcaaacc gacttgagcc gttgtttcac gtcggaaaga gcggtgtaac gcctgccgtt
     4081 atcgctcaga caaatgaggt tttcaataca agagagctga taaagctaaa ggtattgctt
     4141 gaaagcgcac ccgagaagcc gagagagatc gccgaaaaaa tagcggcaga gacaggctca
     4201 caggttgtac aggttatcgg cggcagcatt atactttata aagagaatcc ggatattgaa
     4261 aacggagttg ttaagaaaaa gaaagctccc gaaaagaaaa agtcgggagt cggcaaaggc
     4321 gcaaaggact tcaaaaaagt ttataacgcc aagaaaaagc gtgaagagga acgtcaggcg
     4381 gcggagcgtg caagatacag cagaagccgc agacgaaatt ttaacggcgg ttattcaaaa
     4441 taatttaaag gaggtcgtgg tatggataag aacgacagca aaaccgtagc tcagaacaaa
     4501 aaagcctacc acgattactt tgttcttgag gaatacgagg caggaattga gcttttcgga
     4561 actgaaatta aatcaattcg ccagggcaaa ataaatctca aggacgcatg gtgcaacatc
     4621 gttgacggag aaatattcgt taacggaatg cacatcagcc cttatgacca cggcaatatt
     4681 ttcaaccgtg acccgatgag agtcagaaag cttctgatgc acaagaaaga gatcaacaag
     4741 cttttcggca cggtaaagca ggacggactt tcgctcattc ccctctcggt ttattttaaa
     4801 aaaggcagag cgaaaatcaa ggtcggtctt tgcaagggta aaaaattata tgataagcgt
     4861 gacgtcgccg caaagaagga tgccgcaaga aacatcgaac gtgcgatgaa ggaacgcttt
     4921 caataacatg gggatgaaag gatttcgacg gggattttga accgtgataa gcgagcagtg
     4981 gcgcggaacc actataaaag ccgtagttta taaaaataaa cgacaataat actcagttaa
     5041 tggctgccta agtgcggccc atccttcccg gaagtactgc ggtccggata agggtgtcga
     5101 tcagcagtga acgtgaatga atgagtagcc ttgcaattca tcatgaccta aaggataccg
     5161 taaaggaaag cctgtttatc ggcgctcctt gaggggaatc ttaaaagata aactacgctc
     5221 gtagaaattc gcgggaattg attttcggac gcggtttcga ttaccgccat ctccaccacc
     5281 gataaac
//
LOCUS       DAWBSW010000142         5274 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2860_length_5274_cov_2.462158, whole genome shotgun sequence.
ACCESSION   DAWBSW010000142 DAWBSW010000000
VERSION     DAWBSW010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_5274_cov_2.462158"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3167..3176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1499 a          993 c          992 g         1770 t
ORIGIN      
        1 ccttttaaac agaaagaaaa aggttgaaaa agccttggaa tatgcaaaaa aattgagaga
       61 tgaaggaaag gttttgattg taagtcccga gagtatagga aaaatgagta cacttactaa
      121 agataaagaa gcacttgaca ggctctataa aatcggatat tccgacgctg agaaaataat
      181 tgattttata aaataaaaat aaggctgtga cgaaaattta annnnnnnnn nactacattt
      241 gtcagcaatg aatatgcaga agaaatatca tttgatgttg cagacggatt accgagaacc
      301 gttcctgcat tcatgagcgc agttgaataa gcgttgaaag cagtggtgct gtaatctgac
      361 gaattatagg cagccaaaat agcagcatta taagcagttc ggagttcagt cttatttaca
      421 taatttgcat tgatcgttat gttataataa ttgttatttt gtctttttcc tctgagcgtg
      481 attgtgtaag cagaaatcgt aagcttcttt gtaccttcgg cacaaagctt attctgataa
      541 ttgagactgt tattatatgc acaaccaacc ttagctgaaa gatttgttat tgttgttgtc
      601 gaaccgtcaa cagtctgctt gatatatccg gaagcaacgt cgtcgccctc aatatcgcac
      661 tgccaataac cgattttaag attcggaatt tgattaagat ttgttacacg gcttgagtca
      721 acatagattg tacctgttcc gccgttaacg gtgtgagaat cgtttgcatt tgatttatgg
      781 ctccagcttc cgtattcaac gccgccgtta gtcgtctttg taaaccaatc gcctatacct
      841 gtatttgatg aaggagctga atccgtaaag aaattctttt tacaaatata tgaaccgcct
      901 gttacattgc taaagccaat tgcataaaga ataccctgat tgaaaacgtc tgtgctatat
      961 gtatgagcct gtcttattcc tgcaccgatt tcatttgtat tcggtgagta aacatatgaa
     1021 taagccgttg ctgttctctt ttcaccgttt ttaagggtat atgttgcagt ccaagtgatt
     1081 gtttttcccg aacctgcggc aggtgaagaa taaagcttac cggatgaaac agatgttgaa
     1141 agagttccgt ttgatgagct gtttccgttt gtatagctga ctgttgcgtc gcttacagaa
     1201 atgctgacat ctgttgcttt attgcaattg aagaaaatgt tacctgttgt tttagttgca
     1261 ctgtttgaaa gtgaaccgtc aaccttattc tctctgtcaa cataatattg gaatgacttt
     1321 gttgaagaat ttgtgtaaat ggtttcggga acatagaatg ttgttgtcac aacacctata
     1381 tctgctttga gttcaacatt gattttaatc tgaagtcccg catttacaga agcctgagaa
     1441 atgctgaagc ttcctgtttt tgtctcaaaa cctgttcttg aagctgtgta agtatagttg
     1501 cctgcaccga gcttataata attatccgag ttgcaagctt tgataacatt gccgtttgag
     1561 tcattaacgg ttacagatgt gtctgttgaa ggaagaataa actgtacagt gcttattttt
     1621 gtatcggact ttgttacaag gaacggttga gcatagcaga taccgccgtc gccgagaaca
     1681 taaaatctga catatgaacc gatttcgttt tcaaactcat taagatcaaa tgttattgtt
     1741 tcgttgtcac ggttaacctt cttttcggca acaaccttgc cgtcggcaac catttttaca
     1801 acatttgcat tgtacgggtt aatgctgatc tgatttgtct ctttatcaac attaactctg
     1861 ctgatcatcg gaaattcacc ttgtgcctca aatccgtcac caagctcttg ctcggttttc
     1921 gcagtttttg agcatgcaaa gaatgttccg ttttccatgc ttgtgcgaat atttgcctga
     1981 gtattctcgg gcataacaaa atactgagca ttatttgcaa catcgccgaa atcatgtgca
     2041 tcatcttcgc agaagcccca aatgtttctt ccgagaggtg cggtacgctt gagagtttcg
     2101 tcataaagat agcggtcatt gtgagttctg ttatcctttg tattgacaag ctccataccg
     2161 atacatgccg agtaatttaa gaaaagcttt gaaaactttg ttacaaactc atcattataa
     2221 gtatttatat catgtctgcc ctctgtccat tcgccgacat ggttgatgtg acagataccg
     2281 ccggcagcct gacttttttt tattgcaccc tcgggccatt ttgcatcaag ctcaaggtca
     2341 ccgtgaccgc aatccgcata ataactgtta acatggcatt ttgtagttga tgcaccgtta
     2401 agctcaatac cgagaggaat ctcggtcata ccgtcgccgc ctctgtctga gcctgttgta
     2461 atctgctgat atctttcttc ggaaagctca tcaatatttc cgtgtgaaag atactgatat
     2521 ccaaaaattg aaagacgtgt ctgatccttt gtccaggaat agttaactgt accgtggtct
     2581 gtaagtgcaa gagcctggta gccgagtgaa taatatttct caatcatatc gtcaagttca
     2641 atatcgccgt cgcttctgac ggtatgaaca tgagttgcgc ccttatatgc cttccaggtg
     2701 tcccaattta ctgtttcata cggattggtg atatcaaaat catatttgcc tattatgtcg
     2761 tatgaggaag caaaggctgt tgccgggata taagcaatca tggcaacagc catcattaca
     2821 ctgagaatta cagaaaatat tttacgcatt gaaattcctc caattctttt ccttatcgaa
     2881 tagaatatca ctattaaaca aaatagtcaa taatttttaa taatacttct caaaattata
     2941 ttttatgcac aaatagttac ttttactatc aatgtgtgat gataaaatta tgacctgatg
     3001 ttgaaaattt tataattata tgataatatt ctaatctgta aaaggagcat tgaaatggat
     3061 aaatttataa ttgcacagat tttcggtttt gcaggtctcg cctgttccgt ttccgcttat
     3121 caatgtaaaa agcacaaaag cgttatgatt ttgaaaactt tgaatgnnnn nnnnnngctt
     3181 tttgcttttc aatatttttt tctcggcact tataccggaa tggcgatgaa tataatcagc
     3241 tcagtcagaa atttaacttt ttcctatctt gtaaagaaag aaaagaatac tctccccttc
     3301 cagattttat tttgtatggc atttattgtt tccggaattt ttacatggaa aaattatctt
     3361 agcctgattg taatttttgc aaagcttttg acaacggttg tttatggaat gaagaataca
     3421 aaatatcttc gttttgcgac ggttcctaca agcatttttt ggttgattta taatttcagt
     3481 tgcaaagcta cagccggagt tatctctgaa attttttcgc tgatttcatt gataagcgca
     3541 ataataagaa ttgatattat aggagaaaaa aataaagcag gtcagcaata actgatctgc
     3601 tttattcatt tgcgattttt tataagattc cgaataagta tctgaaaaat gaaattatat
     3661 tttcataaaa ttttctgaaa cctgaagttg ttttttcttc ttttttgtct tcaatttttt
     3721 cctctggatt aggttctttc tcatccggaa tgtcctgtga tattatactg ctgtattcac
     3781 agtaaactga cgaagtttta atactctcgc cttgagaaaa gtttgaaaga agcaatactg
     3841 cctcttctat aattgtaagg tcgtctgtat ttattacgct gtcatggtta cagtcggcag
     3901 caagtctcat tgccggagac ggagtaagca taccgtttat aataaaagaa agtattgttg
     3961 catcacgacc gtcgtaaatt ccgtcaccgt tgatatcgcc gaatactaca accgtatagg
     4021 ttttaagaac atttcctgtt ttatcgtcaa caatttttat ttcatcaccg gttttagctt
     4081 ttgaaatatc ttcaatttca aaatgtgttc cgttctttaa acctattgat ttattaaccg
     4141 gatcggaaat attctcaagt atattaaatc cataaaccaa atcattctgt tttgtaaagg
     4201 aaagaggacc ggaattctta accaactcta tatcaacttt tatttgtaat cctgcatcaa
     4261 cggttgcctg agtaacattt attgttccaa cctttgtttc ataaccgtct ttgacaacgg
     4321 tatatgaata tttacctgcg gaaacacgga aataattttc attatttatt ggatcaataa
     4381 tattgccctg ctcatcttta agagtgagag ttgtatcgga ataaggaata ttaaactgga
     4441 gtgtacattt tgcatattca gtctttgtta caaggaacgg ctgaacatag cagattccgc
     4501 ctttaccgag aatatagata cgaacatatg aattgatctg atcctcataa tcgttaaggt
     4561 cgaatgttat tgtatcatta tcgtttgcaa cgctcttttc accgataacc ttgccgtcgg
     4621 caacgatttt taccgtgtga gcattataag gattgatgct gatttgattc gtttcgttat
     4681 caacatttac tctgctgacc ataggaaacg gtccctgagc ctcaaagccg tcgccgagtt
     4741 cccaatcgtt ctttgatgtt tttgagcatg caaagaatgc tccgttttcc atgcttgtac
     4801 ggatatttgc ctgagtattc ttaggcatta taaaatactg agcattgttt tcaacatcgc
     4861 cgaaatcatg agcatcgtcc tcacagaagc cccaaatatt tctgccttgc ggtgcagtga
     4921 gcttaagagt ttcatcataa agataacggt cgttatgggt tctgttgtcc tttgtattaa
     4981 caagctccat tcctatgcag gcagaatagt taaggaagat acttgcaaat ttttttacga
     5041 actcctcgtt gtaagtgttt atgtcgtgtc tgccttctgt ccactcaccg acatggttta
     5101 tgtgacaaat tccgccggca gcctgactct tttttaccgc atcctcaggc cactttgctt
     5161 taagctcaag gtcaccgtga ccgcaatcgg cataatagct gttaacgtgg cattttgtgg
     5221 ttgatgcacc gttaagctca ataccgagag gaatctcggt cataccgtcg ccgc
//
LOCUS       DAWBSW010000143         5209 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2888_length_5209_cov_2.567520, whole genome shotgun sequence.
ACCESSION   DAWBSW010000143 DAWBSW010000000
VERSION     DAWBSW010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2888_length_5209_cov_2.567520"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    449..458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3045..3054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3669..3678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1464 a         1063 c         1320 g         1332 t
ORIGIN      
        1 acttaatccc gttgcctgta aagataggca cacttgccgt tgttcttgcc gtatatttgt
       61 tctttgtatg gcacaacatg atgcttctga ggaaatacag atggacggct gttctgtatt
      121 ttatcttggc attggcggtt atcgttcttg taatcttcga tttcggcaat gtatggatta
      181 cgctgaacaa ctggctgaac ggaattattg attacctttt ttataatatg catatgagat
      241 aattgaaaaa gaattgcaac gtcggagatt tccggcgttt ttctttaaat tttttgaaaa
      301 aactattgga agattgcaat aaatattgta taataaacca tgtatggaac aaatatgctt
      361 tatggagtgg tcttatggcg gctaaatata ccatatcact taaaaagata atagaggtca
      421 tcggatttga tattgaatac acacccgtnn nnnnnnnnct tatgctttcc ggatttacaa
      481 aatattttga cccggaacgt attcaaatta tgggtgtttc cgagattgaa tatcttaatt
      541 cattcaagcc tgaggacagg ttcagaaagg ttaaaagact tctttcaaaa aagcccgttg
      601 caatcattct gactcgtgac cagaattatc acgatgacac tatcgagctg gctaagaaat
      661 acggcgttgc gttgctcaaa acaaccgacg gaacgtcaag ctgcatgtca actttgatat
      721 cctatctgag cgttgagctt gcgccgagaa tcacacgtca cggcgttttt gttgaggtct
      781 acggcgaggg cgttctgata atgggcgaga gcggcatggg taagagtgaa acggcgcttg
      841 agcttatcaa gcgaggacac agactggttg ccgacgacgc tgttgaaatt cgaaaggtgt
      901 cgcataaaac attagtcgga tcggcaccgg aaaatatacg gcattttatt gagctgaggg
      961 gagtcggcgt cgtcaacgca aggcggcttt tcggcatcgg ttcggttaag gtttccgaga
     1021 aaataaacat ggtcattcag cttgaaccgt ggaacgccga gaaaaattac gagcgtctcg
     1081 gacttgataa cgaaacgatt gatatactcg gaattgatat tccgatatgc gttgttcccg
     1141 tcaccccggg tcgtaacatc tcaatcatca ttgaagcggc ggctatgaac aacaggcaga
     1201 agaaaatggg ttacaatgca gcaaagcagc tccttcacaa cctcggtatg acagacgaca
     1261 tcaaggttga ggaaaatgag cttgaaattt ggcataactt ttaaagcgca aattgcttaa
     1321 ataacggagg aatttacaat gtacaaggtt ggagttgacc tcggcggaac aaatattgcc
     1381 gtcggagtcg ttgatgaaaa atacagtatt ataggcaagg gcaaggttaa aacaaatgcg
     1441 ccccgtccgg cagaggaaat ttttgccgac ataaaaaagg ctattgatat ggctgtagcc
     1501 gacgcaggac ttactatggc tgatattgaa agcgtaggcg tcggaacgcc gggttcggtt
     1561 aatcaggaca cgggcatgat tgagttctcg aacaatcttg cttttaacaa tgttcctgcc
     1621 aagaaaatgc tggaggagct cacgggcaag ccgtgctatt ttgagaacga cgccaatgcc
     1681 gctgctctcg gcgaggcata cgcaggtgcc ggtaacggcg caaaaaactt tgttgccgta
     1741 acgctcggaa caggcgtcgg cagcggaatt attgtggacg gcaagattgt cagcggcatg
     1801 aactttgccg gcggcgagat gggtcacaca gttatcgttg ttgacggaat tccctgcaac
     1861 tgcggacgta acggctgttg ggagagctat gcttcggcaa ccgctctcat tgctcagaca
     1921 aaggcaaaga tgaaggagtg tccggacagt aagatgtggg agctttgcgg cggagatatt
     1981 aacaaggtca gcggcagaac ctcattcaac gctatgtatg caggtgacga agcaggcagg
     2041 gaggttgttg aaagatacca atattatgtt gctgtcggca ttatcaatat aatcaatatt
     2101 ttccagcccg aggtactttg cgtcggcggc ggaatcagca acgagggcga taaccttctt
     2161 gacccgatca gagaatatgt taaaaaagaa agatacagca agcatgccaa gaagcagact
     2221 gaaatctgca aggcggttct cggtaatgat gccggaatca tcggcgcagc gttgttggga
     2281 taaaagcaaa gaagatttga gatttattgt gagtctaaag tctaacatct aaaatctaag
     2341 gtctatcttg ggtgacatta tgagcttcga taagattttt gaatacgctg tgagcatcgg
     2401 ctgtaaagcc gagcgtgaag ttccgatgag caagtacacg acattccgta tcggcggaaa
     2461 tgcgtcggtt atgctcactc cgctgaacga cgggcagctc gctttgatta tcaaggaatg
     2521 taaaaaagag aatatcaagc cgttcattct cggcaacgga agtaatatgc ttatctgcga
     2581 tgccggtctg aaaacggttg ttgtaaatat gtgccgcccc gagccggaaa tcaagcttgt
     2641 gaacggcgat acaatagttt gcgacgcagg catgactatg agtaaggtct gcaattttgc
     2701 gcttgaaaac ggtcttacgg gcttggaatt tgcattcgga atccccggca gtgcaggcgg
     2761 cgcggcatat atgaacgcag gcgcttacgg cggagaaatg aaggacgtgc tcgttgagtg
     2821 ccgtcacatt gaccccgacg gaaatctcgg atcgctcaag ggtgatgagc ttggacttgc
     2881 ctacagaacg agcgcatatg agcataacgg ctttattata acaacgcttg ttatgaaact
     2941 gaaaaagggc gacaaatcgg aaatcaaatc caaaatgcag gagcttttgc agcgccgtaa
     3001 ggataaacag ccgcttgaat ttccgagcgc aggcagtaca ttcannnnnn nnnnacattc
     3061 aagcgacctg taggctattt tgcaggcggt ttgatcgagg aatgcggtct caagggcttt
     3121 tctgtcggcg gagctcaggt cagcgagaag cacgcaggct ttatcataaa caagggcgga
     3181 gcgaccgctc aagacgttct tgacctcatt aaatatgtac aggacagggt ttatgaagag
     3241 aagggtgtac atctcgaacc cgaagtcaga ctcattacgg ttggctgatc tttttccgtg
     3301 atacgaacca aatccctctc ggcatataag aatatagcct tcgaggaatt tgctccgtct
     3361 aacgaaaaaa tctcaagcct tgattttata gcgttcaaac agcacccaat ctgcaaaatg
     3421 cattgtgaac aattttgtct caacctcaga ctgagtattg gttggcacaa tgtccaaagc
     3481 acgttttcgc ttggaacagc gcaaaagtac agctcccaat ctgcaaaatg cactgtgaac
     3541 aattttgtct caacctcaga ctgagtattg gttggcacaa tgctcacggt aaattttaaa
     3601 atgcagaaga aatttttcaa ggcttcccct tgagggtagc gaagcggcgc gacggtagtg
     3661 aatgaacgnn nnnnnnnngt ggacgttcag agccggagcg tgaccgagcc gcagcgagac
     3721 gctccgtcgt aagacggtga tgaggtggaa aagttcgggc tttatgttat tatataatga
     3781 gatgaaaatt tgccacctca tccgagcggc gcaatccgcc gcccaccttc ccctcaaggg
     3841 gaaggctgat gaatgactgc aaaatttgct ttttgttttg tagaaattgc tttaagcaaa
     3901 agcacaacca ccactgttta caccttgaaa ggaatggttt tatggaatta ttgattgtta
     3961 cgggaatgtc aggcgcaggt aaatctgccg ttgttgacgc tttggaggat ctcggctatt
     4021 tctgcgccga taaccttccg cctgcaatta ttccgaccat tgctcagttt gtaagcaaga
     4081 cggataacgc gcagaagatc gcaacggtta cggatatccg tgtcggcgaa aaaatgttta
     4141 acgagttccc gagtgttctt aaaaatcttg aggaacaggg ctataagtat aaggtgcttt
     4201 ttgttgatgc ttccgaggag gttcttgtca gaagatataa ggaaacaaga agaaagcatc
     4261 ccttacttga caaatgtgac ggctcgcttc atgctgctat agcaatggag catgaaaagc
     4321 ttctcaatat cagaatgaag gccgattata ttattgatac atcaaagtcg agcgttgccg
     4381 agtgcaagca gagggtaaac gagctttttc ttgacaatcc ggacagtgca ttgaaaattc
     4441 gctgtatgtc ctttggcttc aaatacggaa ttccgaacga cgccgacttg gtttttgacg
     4501 ttcgctgtct gcctaatcct ttctatgtgc cgtcgatgaa atacaggaca ggtcttgaac
     4561 ccgacgtcag cgagtatgtc atgaattccg atcattcggt taacgtgctt aacaagctca
     4621 acgatcttat tgattacact gttcctcttt atatcgagga gggcaagagt cagcttgtta
     4681 tcgcaatcgg ctgcacgggc ggacggcacc gttctgtctg cttcgccgag aaaataagag
     4741 aaaatctgct gaagctcggc tacagcgtca gcgttaaaca cagagatata gaaaaataac
     4801 aggggtataa gcagatgtca ttcaccgcag gagtgaaaaa tgaattagca acgcttgcgt
     4861 tcgagtccga atgttgcaag cgttcctttg tgtacggttt tttactgtac ggaaagagct
     4921 ttacggcacg ttcggtaagc tgtgcgacgg attacgccga ggttatgcag gcttttgaaa
     4981 gaacaattgc cgaaacttcc ggagtcaaaa cgacggttac tcggcttaga agcggaaaat
     5041 atgttctgaa aattccggca gttgaggaca gaaagcgcat tatcgagtat ttcgaccatg
     5101 ccgtcaattc aatctcactc aggatcaaca gaggagtttt tgaggatgac gagtgctacg
     5161 ggtcattctt gcgcggggtt tttctctcgt gcggttcaat tgccaaccc
//
LOCUS       DAWBSW010000144         5159 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2920_length_5159_cov_2.228840, whole genome shotgun sequence.
ACCESSION   DAWBSW010000144 DAWBSW010000000
VERSION     DAWBSW010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2920_length_5159_cov_2.228840"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4162..4171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1448 a         1148 c         1039 g         1514 t
ORIGIN      
        1 ggctattata acgaaaacaa tcaacccgaa agatttgaat attacacggg cggtaagctt
       61 agttattatt atatttcaag tgattttgac gataacggaa atgataacgt tcagaaatat
      121 tacagtgccg atggcaaact tctgggaaca gtcaaaaacg gcaaattcta taattcaagc
      181 ggtaaggaaa tttcggaaag cgaaatggat gcgcttcttc cgcaataaaa aatcggcaga
      241 gacacggcaa aatgttatgt ctctgctgtt tttttaccat ttattatgaa ctctttcggc
      301 aaaaacgacc ttgtctttaa cttcaatcac ggtgccgtca tcgaggattg ctttgcccga
      361 aattttgccg aaaacctgat gcggaagcat acagataagc ttgacgtcaa gcggagctac
      421 acggtcaata ataggctcaa agctcatctc aaatcttccg tcgttgctcg taaacgtcca
      481 cggctcggtg taatagtatt ctccgtttct gtacggtata tgaaattcaa cctgatcgag
      541 cttgtgcgat tttccgttat agaaaagcat attttcgctg gctgccgagg tatcgccgaa
      601 gccgtatccg atattgaatc cgaagcggct gccgtcatcg aggatcatct gtccgctgcc
      661 ccagtaccac gtattgtcat aggtccacac tcccctgccc caatcgagcg tagcaagcga
      721 ctgattttcc tcattgaagc tccagcgaag atcgccgaaa ctcatcttgc ccgttgctct
      781 catgcagttg attttctgat tatagtagaa gtgtttatcc ttatcaaacg gagttgcaat
      841 taccatgctt tcctcgggaa tatcggtaag cgtgacgtca aaagtcagga gcttacccga
      901 attgcaatag ttgccgtatg taccgtaaag acgacgtgtt ttgccgtcat ttttaaaggt
      961 catgtcgcag gtacccacct tggcatggat atcacccttt tcgcttgtcg agggcaggtt
     1021 cattttaccc atcgggaatg caccgatttc actgtcattc atctggaacg gcttttcgcc
     1081 gaagcccatg agcgaaatgc tcaggcagct tacgtatccg ctgtcggaaa tcgtgaggca
     1141 aagcccgaag tcctgattgc cgatatagta gtagtcccac tccttaattc gaaatttagg
     1201 cgctttgata tcgtttctgc tgtactccca gtagagcttt ttcgcaaatc cgggttcggt
     1261 aatattgccg tttgcgttta aaagcttttg ctttgaagtg atttcatgct gcataaactc
     1321 tgacataatt atcccctctt tattttttca tttctgcaag aattctatag tttatcagct
     1381 taaagccgag cgactcaatg tgctgcttcg tctgcggatc cttgagcaat ttatattccc
     1441 actcacgtct gaaccaaacg ccggaagctc ctttaagatc ctccgtcggg aacgacggat
     1501 gcatataagt ctcggttact gctccgtcgg gaaaagtctt gtaaagctca taaatatatt
     1561 ccttaaaatt atcatagctt tcgctctgag ggccgatcca ctcgttcgga atcagataat
     1621 cgggaatagc aacgtgaagc tttcttgcct tatccacaac attgccgagg agtccgagga
     1681 cggtttcttt atcaacctga atgtcaagca tcgaatttga aaactgctca tctgcaagat
     1741 ttgcaggaaa tctgaaaggc agaccgtact cggcggcaac cttaaaggca acattcaaaa
     1801 gctcaaagcg acctgttgca acgccgtaaa ttgatcccat gtgattatca atgtgagacg
     1861 gattcaagcc gagcttttta agtaattcga tttgggcgac tgcctcggca taaacctcat
     1921 caagacttgc gtgcttctca aactcatcac tttcatggtg catatagccg aattcgtcgc
     1981 gtagactttc tgtattgttg tgagccacgg gaccccaacg gtagctgctc cattcatttg
     2041 taaaagtcca gtgaacgccg atacaaagct cgggattttt cacggcaaaa ttaaccgcgt
     2101 cgggtgccca cggacacgga gccatgattg ttgacgacgt cagtgcgctg ttcgggtcgg
     2161 tgagcaaatc tgatattgcg agattggcac ctttaaaaga cccgaaatcg tcggcattga
     2221 ttataaggta tttagacatt tatttcacgc tctcttttgc atgatttctc tcggcgattt
     2281 ccgcaaccat ttgctgctgt ctcttttcgg tgatgttata gaaaagcata ggaataatag
     2341 ttgccgccat gctgattgca cccgaaagaa taagaatgtt ccaaagaagc tgtctgccgt
     2401 ctgctgtgat tgccggtgtt gcttcggggt tgatgcctgc ccagctatag gagaatacgc
     2461 cgacgaaagc accgactgcc atgccgattt tattgataag cgtctgcatc gcaaagcaga
     2521 taccctctgc tctctcgccc gttttccatt caagataatc aacggtatcg cctatcatag
     2581 cataagtgat aatgttgttt acgccctgag gaataccgag aaggacaaga ccgattgcat
     2641 taacaacaag cttccacggt gcatcgtagc cgatgaagta cattgcaaac attacaactg
     2701 caccgagaat atgaacataa atatatgccc acttctttgt aaacttcttc gtcatccagg
     2761 gaacaagaat tgaagcgacg agtccgccgg gaactataag catcgttata aggctgaaaa
     2821 gtccctcgtt attaagaaca tattttgcga aatacagtcc tcccgtataa gtataaacgg
     2881 ttcttgcgcc gccgagaata cccgaaataa caaccagaag cagttgctta ttcttgaaaa
     2941 gaagcttgag gttgtgtccg agtgaaggag gagcatcctt tgaagtaaat ctttctgttg
     3001 tgtttttaaa gccgtaataa aacatcggaa cagaaacaac aacgagcaca agcgcacaaa
     3061 tgaaatatgt ccatttaagc gtttctgcat tctgagaaat atactgcgga agaacatttc
     3121 cttctgcgtc tttgaacttt gctgtaacgg cgtttgtaat tagaggtacg ccgacggtta
     3181 caaggcctgc gccgagcgtg cagacaagac gcgcaaacgt cagcacctta cctctgacga
     3241 ccgtatcgtt tgtcatgcag gttgagagtc cccagtaagg aacatcggca acggtataaa
     3301 ccattgacca aataacataa agaacggaaa taacaacaaa ggttgacttc gttttcggct
     3361 caaggaacgg gcagaaacaa agaactgtcg taatagcaat aggaattgcc atccatttaa
     3421 gatatgggcg gcattttccc catttagttc ttgttttgtc aactatagag cccatgatcg
     3481 gatcattgag cgcatcgtat attcttgtaa agagcataag gaatgcaaca gccattgcgc
     3541 ccgacaaact cttgaatacc gtgccctttt caacagcgcc gaaaagcagc gcatctgtca
     3601 taaaaacagt aagaaacgag ccgatgatcg tacatatgaa attctgtccg aatcctgtta
     3661 cgctgtatgc gataacctcc ttcggctgaa taccctcacc gggcgttgtt ccgaacatat
     3721 tgtgccaaaa attctttttt gccatcagaa aaccttcttc ctgtcgtgtg acatacgctc
     3781 acactttatt gtatataatt ttatcacatc agattacctt ttgtaaatta aaaaaataaa
     3841 aacctacttt tcatacaaaa acaaagcagg ttttttatta tatattacta tagataatct
     3901 attatttttc ttttaaaaac tgtataattt aaacaattta ttgagattgt tctgattttt
     3961 cggatatgat ttcttttaat tgctcgtata tctcgacaac cttgaatctc ggctcgtatt
     4021 tcggcttgct ttcaacaagc tttatttcct tctcggagaa tacattttcg atttcatctt
     4081 ttttatccgc cgcgggaaca cacatcggag gaaacatcac gcaccaccaa ttatgaccct
     4141 cgcccgagcc gattacaact cnnnnnnnnn ntcaaagcaa gatatttgcc tgccggaagc
     4201 gtcacggttt cataggttct tgttgtgaaa tattcgtttg aaaggctgat tttaacgtca
     4261 tagtcaaaac ctgcctgatt cacaaatgcc tttgccgatt tttcaagctc aggcagtacg
     4321 ggttctattt ttttaactgc attttcgacg ttgacgctgc cgttaaatat atccttgcct
     4381 tcttgaagaa tataatcacg cagtcttagt tttaaattct gatcaacgct tgtatcggaa
     4441 tttgctataa cgtgaagtct gagaacatcg gagcgaatct cttccgatgt tttcgcaaag
     4501 gaacaaattg aaaaaagaat tgcaacaacg attccgacgg taacggatat tttaattttt
     4561 tttgataaag acatacagtc aactccttac ttgatttgca agtaaattgt tgactgtaat
     4621 ttttcgtttt attcatacat tgccagaatt tggctcgcct tttctttaac ggctcttgca
     4681 agcgcttttg gctgaataac cctgatatcc gtgccgtatt gcaaaatcca gcttacaagt
     4741 ccttcactca tctcgacctc agcctttatg cgaaaatgtt cattatcgtc aatttttatc
     4801 ggaacttcgt ctccgaactt ttctataatc tcatcgagaa tcgaatttga gcatgaaaga
     4861 gtaacgtttg cttcctctcc ggagaacata ttaaaaagct tattcgaata atcggcaatg
     4921 tcaaacttgt ctgtatattg agaaaccttt gaaaaatgct ttgcgggtga atcaagctgt
     4981 tcaatctcaa taattctgtc aagacgcaga tgcatgagat ttgaatactt attgttattg
     5041 caaatcaggt aataatggtc gttagaccaa agcatcgcat aaggatttac aagaaaagtc
     5101 ttaccctcgt tacgaacaag atatttattt tctattgaac gtcttgcata tctgagcat
//
LOCUS       DAWBSW010000145         5128 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2941_length_5128_cov_3.059137, whole genome shotgun sequence.
ACCESSION   DAWBSW010000145 DAWBSW010000000
VERSION     DAWBSW010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2941_length_5128_cov_3.059137"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1343..1352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1557 a         1077 c         1168 g         1316 t
ORIGIN      
        1 atacaagagg cggcactccc gttgccgaca agacgctcta ctgggacgat aaggttctcg
       61 acggtgttgt tcctccgaca aaagagggag gatttgaatt catcggctgg aacattgttt
      121 ctccgaaatt gagcgtaaca actttgaatt gcacatacgg cgagcttgtt caggacgaca
      181 gcattgacac ggttacgctc ggtgcggttt ggagagatac cgaaaagccg gtaataatcg
      241 gtcttgaaaa cggcaagatt tattgcggac ctcagaagtt taaggtaacc gacaatgcca
      301 catcgggaac gacaatcgta ataataaacg gcaaaaatgt tgagccggac gcagacggat
      361 attatacggc tgaacccgca aacggtgagt tcaagattat tgctgttgat tcttttgcta
      421 acatggcaat ggtccaaatt accgtaaacg ccgctcacga ttacagcgat tggaagttcg
      481 acgagaacga gcactggaag gagtgcaagt acggcgattc cgtaatcgaa aagggtgagc
      541 atacctttga atgggtgacg gataaggaag cgaccgagac ggaaaaaggt atcgcacatg
      601 aggaatgtac ggtctgccac gcaaagcgca acgagaatac cgaggttcag aagctcaaga
      661 caaaatggga aaaaatccgt gattggctta tctcgattct tgtgaagata atcaagtggt
      721 ttatcgacct tattaaagat gtttgctgaa cggcaacaaa aactacagct aaataaacaa
      781 attgcgtctc ctcttgattt tttcaagggg agacaacgct tttagcttat gatttgttcg
      841 atcggcggat tacattgttt aatcaacgat aagcttctga ttgttcgtta attgtcgaat
      901 aggtgataaa aaactaagat atgacaactt tttcctctgc caaacctact aaaaaacacc
      961 tcaaatatta ccaatttgtt aattctttta tttacccctt gcaaaatagc ataaatagga
     1021 ctatactaaa ttagcactca gcaataaaga gtgctaattt tcttattaca ttacagacag
     1081 gtgatatata tgcgtacaaa acagttcaag gcagagtcaa aaaagctgct tgacatgatg
     1141 atcaactcaa tctatactca caaggaaatt tttcttcgtg agcttatatc aaatgcgagc
     1201 gacgcaatgg acaagctcta ttatcattca ttgcagaata acctcgctct caacagaagt
     1261 gacctcacaa tcaaaattca gcccgataag gaggcaagaa ccctcacgat ttccgataac
     1321 ggtatcggca tgaacggcga ggnnnnnnnn nnataacgag aagaaagagg acgttgacat
     1381 catcggtcag ttcggcgttg gcttctattc cgcattcatg gtcagcgaca agattgaggt
     1441 agtcagcaag gctttcggcg acagcgaggc tcataaatgg acctcaaagg gtgcagacgg
     1501 ctacacggtt gagccgtgcg aggccgactt tgaccacggc accgtaatca ccctttatct
     1561 aaaggagaat acagatgacg atgattatga gcagtacctt gaagaataca agatcaaaga
     1621 aatcgtgaag aaatattcag attatataag atatccgatt acaatggact gcacccacag
     1681 ccatctcaaa gagggcacgg aggacgagta tgagcaggta acggagaccg aaacgttaaa
     1741 ttccatggtt ccgatctgga aaaagaataa aaacgaggtc acggctgacg aatacagcag
     1801 cttctacaag aataagttct atgactatga ggagccggca aaggttatcc acgctaagat
     1861 ggaaggtcag gtaatgtaca atacactgct ttatattccg cgccatgctc cgtttgatta
     1921 ttacacaaag aactacgaaa agggcttgca gctctattca aacggtgttc tcatcatgga
     1981 caagtgtgcc gatctgcttc ccgattattt cagctttgta aagggtcttg ttgacagcga
     2041 ggatctgtcg ctcaacatct cccgtgaaat gctccagcat gatgcacagc ttaagcttat
     2101 cgcaaagagc cttgaaaaga agattcattc cgagctgatc aagctgatga aggattctcg
     2161 tgatacctac gaaatggttt ataatgcatt cggcactcaa ctcaaattcg gcatttataa
     2221 cgccttcggc gcaaagaacg atacgctcag tgatcttttg atgttccact catcgaacga
     2281 taagaagctg acaacgctca aggaatatgt cggcagaatg aaagagtcac aggatacgat
     2341 ttactatgca tgcggcgaat ccgttgcaaa gatcgagctt cttccgcagg ttgagtctgt
     2401 cattgacaag ggctatgagg ttctctattt caccgagaat gtcgatgaat ttgcaattaa
     2461 gatgctcaat gagtttgagg gaaagaagtt tgcaaacatc tgcaccgaca gcattgagct
     2521 tgacagtgaa gaggagaaag aggagcttaa aaagaagaat gaggaaggca aggagatgct
     2581 cgattacctt aaggagagca tcggtgacgg cgtttcttcc gtaagattta caaataagct
     2641 caaaaaacac cccgtatgtc ttacaactga gggcgaaatc tcactcgaaa tggagaaggt
     2701 tctcaattca atgccgacca atgagcagaa ggttaaggca aatatcgttc ttgaaatcaa
     2761 tgagaatcac ccgattgcgc agaagcttac cgagctttat gagagcgata aggagaaggc
     2821 aggcaagtac gcaaagatcc tttacgctca ggcacgcttg attgagggcc tttcagtcga
     2881 tgacccgacg gagctgagca acctggtatg cgatttgatg gtatgatata acagcttcaa
     2941 gcaccgctga tatttcggcg gtgcttaatt ttttatgctt tacggttaga ttcataaggt
     3001 cgtaccctaa tcattacttg cgcaacttgc acaaaaaaac aatatgtttt ttgtgatata
     3061 gttactttac ttttctttgc aaaaaaagta taatatagta aacatttatg atctcgaaag
     3121 gggcagaata ttatgagcaa gaagaagaaa gtctcggcag atccgaacga ggaagttgtt
     3181 gagaccaaga aggcaaaaaa agaaaagaaa gctaaaaagg aaaagactcc aatggatctt
     3241 gattccaaga aaacgcttgc aaagagcatt acagctctcg tttgcgttat cgctgtttgc
     3301 gttacggctg ttgtaaacac cggtaagatt tgcgacgctg caaagaaagc ggcagagcag
     3361 agaggcggcg cttcagctac cgatacggtg ggcgaagacg ttgataacag cggcgaggtt
     3421 ccgacagacg ccaccggcga agttccgaca gatgctaacg gcgaggttcc gacagacgct
     3481 aacggcgcag atgtaacaaa tgctgccggc ggtaacagca gcacaggcgg aagcacgggc
     3541 ggctcggcat ccaaaacaaa cgcagctaat gcaggttctt caatcccgac aggcacagct
     3601 cagatcgtta gctattacaa cacggctacg gcaaaggttg cctctaagaa ggcagctttc
     3661 aacaagacaa gaacaacaac cgagaagtca tatgatgccg gtgttgctct caagtccatg
     3721 aagagcattg tttataagtt catgggcgta ggcgattcca acaagttttc aaagtccgtt
     3781 acttcggcag acgctggaag ctaccataag tattttaagg catccaagct tacaacctca
     3841 gacgttacaa gcgcaacatg cgtaaagaac ggcaataact acacaatcac gcttcatatc
     3901 aagaacggat caagcaccgt taagggcggc aaggttgttt cttccaacaa tgctcctctt
     3961 gacagaagcg gacttgcatg cggcgagaag gataaggatt attgggatca caagacagcg
     4021 gagaacgtta tgtctgcaat ttccgaggtt ccgggatgcg gcggcgctaa tatctcagag
     4081 agctactcta acgctgttat taaggctgtt gtaaatgctt cgacaggtaa tctcgtttcc
     4141 ctcaacgcca agttcgattt taagtttgag ctttcaagca ttatgagctc atcgggaacg
     4201 gctgttgcat cgtctgttgt taacatgaca ggtttcaaga tgtaatctcg ataacagaat
     4261 acataattaa ggcacggcga aattgccgtg ccttttcttt gttgtacaga tttttgttaa
     4321 tgcgtcgcat tgttatgtat taactgcttt ttattttaac aaccgcaagt attctttctt
     4381 aaaacggagt tagctaagac tataatagct gttaagcgtc aattttatga ccatgtttaa
     4441 cacttatatt taaaaagact atcgtatttt tcgatagaat tatgcaatac ttgcctttaa
     4501 atatgtataa gaaaagagtc gatttctcga ctcttaccac agatataaca ttgggaaaca
     4561 gttttgaaaa taattcttta atacttattc ccaatcttgc gaatacacag tatagtatga
     4621 cctgatttgc caatataccg gaatcagcag aaaatataat tctgtttagc atctctaata
     4681 ctttttgtgt tattccaatg tggactttct ctgcagaaaa ataagaaagt acggtagccc
     4741 tttgtatcat ggactaccgt acttcgtggc ggagagttag ggattttttc ggtgcactct
     4801 tgtgcacctt cgcgtcgatt cgctccttga ccccgtcggc aaaaacagtc caccggactg
     4861 ttttcttccg cagacttcgt ctgctccctc cttgttcgaa tccctttgtt tatttttgcc
     4921 aaagcaaaaa gcaggtctcg cgacctgctt ttcattttgg cggagagtta gggatttttt
     4981 cggtgcactc ccgtgcacct tcgcgtcgct tcgctccttg accacgtcgg caaaaacagt
     5041 ccaccggact gttttcttcc gcagactgcg tctgctccct ccttgttcga atccctcttt
     5101 ttgttttgcc aaagcaaaaa gcaggcct
//
LOCUS       DAWBSW010000146         5121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2948_length_5121_cov_2.173510, whole genome shotgun sequence.
ACCESSION   DAWBSW010000146 DAWBSW010000000
VERSION     DAWBSW010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2948_length_5121_cov_2.173510"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1397..1408
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1235 a         1206 c         1189 g         1479 t
ORIGIN      
        1 cctccatggg cttccgataa taaccctcgc cgaaggtcac gtccgtaagc tccgtggaaa
       61 aggtcgtgtg ccggcaatga tccgaccaat aggtatcaat cattcgtatt tcggtgattg
      121 tcggatttct gttttcggat ttaaaataat tctgacagaa cttgatatcg tcaatatcca
      181 ttgcaagacc gaaatcttca acaaattttt caagctccga gtcggttaaa tcaaggaagc
      241 cgtcaagagt ttcgacggat gtcggaatat catactttgc gacaagagtt tcgggctttg
      301 tgagtgaagc ctctctcgct tcgacggggt taattacata ttttttgatt gtatcaattt
      361 cgcctgcgga aagctcgccc ttgagcaaat aaaccttcgc tgtacgcacg gtcggacgtt
      421 cggattttga aataatctgt atgcactgtg cggcggagtc ggctctctgg tcaaactgac
      481 cgggaagata ttcaaccgca aagcacgaat agccttcgga atttatcacg tccgaaacta
      541 tatcgagctg aggctcggaa aaaaccgtct taacagcata attaaaaagc tcctcggtga
      601 tgttttcggc atcgtaacgg ttgataattc tgacgtcctc gagacccttt atcgagagaa
      661 gctcattggc ttctttaagc aggctctttg cctcatgggc aatttccggc ttcttttcaa
      721 cataaactct gtaaaccata ttattcctcc gttgctttca aggctctttg accgatgtca
      781 tgtctgtaat atgcgttttc aaatgaaatt tctttaacct ttttgtatga gctgtcgatt
      841 gcttccttca gcgtcgaagc aacggatgtt actcccagca ctctgccgcc tgaggtgtaa
      901 agctttccgt ccttttgcgt tgcgcctgca acataaacgg aatcgaatat ttcttcggga
      961 attgtgattt catatccttt tttatacgaa acggggtagc cgtccgaagc catgataacg
     1021 cagcaggcgt cagcgtcctt gaatttgacg tcagtttccg acagagttcc gtctgttgtc
     1081 gccttcataa cagtgagcag gtcactttca agcagcggaa gaactacctg agtttcagga
     1141 tcaccgaagc ggcagttata ttcaattacc ttcgggccgt cattagtgag cataagtccg
     1201 aagtataaac agcccttgaa ggttcgaccc tctcggttca tggcgttgat cgtcgggaca
     1261 aaaattgtgt ccatacaaat ttttgcaacc ttatctgtat aatacggatt cggagcgatt
     1321 gtgcccatac ctccggtatt gagtcccgtg tcattgtcgc ccgaacgctt gtgatccatt
     1381 gaggaaacca tcggctnnnn nnnnnnnncg tcggtaaacg caagaacgga gatttccggt
     1441 ccttcaaggt attcttcaat tacgatgctt tctccgctcc tgccgaattt tttgtcctgc
     1501 attgcttcaa cgacggcttt cttggcctcc tcacgggtaa atgcgaccgt aacgcccttg
     1561 ccgagagcaa gaccgtcggc tttgataaca gtcgggatag gggcattctc aaggtaagcg
     1621 agggcttttt ccatatcgtt aaatgtctcg tatgaagcgg tcggaatact gtatttcttc
     1681 ataagatttt ttgaaaagac cttactgccc tcgattattg cggcatttgc gcgagggcca
     1741 aagcacggga tcccgatttc ctcaagcctg tcaaccgctc cgagaacaag cggatcgtcg
     1801 ggagcgacaa cggcataatc aattttttcg cttgacgcaa agtcaacgat tttatcaatt
     1861 tcacatgctc cgatgggaat gcagacagcg tcgctttcaa ttccgccgtt tccgggcaga
     1921 gcatatattt tatcaattgc tttattttct tttaattttt ttattatggc gtgttcacgg
     1981 cctccgccgc cgataaccat tattttcatt gcttatcata tcctttcaag cggaaaaaga
     2041 accgttaaaa atcagtggtg gaacagtctc attcccgtaa acgcaatcgc catgtcatat
     2101 ttatcggcac attcgattac caggttgtcg cgaatcgagc cgccgggttc ggcaatatat
     2161 tttacgccgc tgagctttgc tctctcaata ttgtcgctga acgggaagaa agcatccgag
     2221 ccgagcgaaa ctccgttgac agtgtcgaga tattcacgta cctccgattc tgtaagcggt
     2281 tcgggacggg tggtaaaata tttctgccag ttaccctcgg cacaaacatc ttcctcgcat
     2341 ttattgatat atctgtcaat tacgttgtca cgctcgggtc tgccgagagt ggggagaagg
     2401 ggaagattaa gaaccctgtc gctctggcga aggaaccatg tgtccgcctt tccgccggca
     2461 agacgggtgc agtgaattct cgactgctga cctgcgccga cgccgatagc ctgtccgtca
     2521 taggcaaagc acacggaatt ggactgcgta tatttgagcg ttataagtga aataatcagg
     2581 tcacgaattg catcgtcggg gatattctta tttttggtga caacattgga aagaagctcc
     2641 ctgttgattt caaaattatt tcttccctgc tcaaaggtga tgccgaacac ctgcttgcgc
     2701 tcaatcggct cgggtgtgta gtcggggtcg attttgacga tgttgtagct gcccttgcgc
     2761 ttcgatttga ggatttcaag agcttcgggc tcatagtcgg gggcaattat tccgtcggaa
     2821 acctcacgct tgatgagctt ggccgttgtg acgtcgcaaa catcggagag agcaacccaa
     2881 tctccgaacg agcacatacg gtcggttcct cttgcacggg cataggcaca ggcgagaggg
     2941 gagctgtcaa ggtctttgat atcgtcaacg aagcaagcct ttttgagctt ttccggaagc
     3001 tttattccga cggccgcaga ggtcgggctg acgtgcttaa atgatgcaac ggcgggaagg
     3061 ccgagagcct cctttaattc cttaacaagc tgccatgagt tgaatgcgtc aaggaaattg
     3121 atatatccgg gcttgccgca aaggatttcg atgggaaggt cgcttgcgtt attcataaat
     3181 attcttgaag gcttctgatt ggggttacag ccgtatttga gctcgaattc tttcatatat
     3241 ttccgttcct ttcttggttg ttttcagcaa ttcttgttta taagcttttc ctcatagctg
     3301 ccgtctgtga ggtcggtgta tcttacataa agggaaatct tattgttctc gtcaagataa
     3361 ttccagattt catcggcaaa ttcgtcaata ctgtcgggga tagctactct ctcgggctca
     3421 cccttaaatg ttgggatcgg gttgccgtcc gtcacatatg tatggataaa gtgaccgagc
     3481 cctgcctttg acggatacga gaaggtgtat ctagcacagt cgcttccgtt ttcatcaagg
     3541 cttttaagta tgctcatttc atacttaaaa tcattttcgc cgaaggtcag cattccgctg
     3601 attcggggag tgaagttcgg cgcatccggc tcaaactcac ggctttcaag gctttcggaa
     3661 aagcttttgc ccgttacaat tccgtcgaaa accgtgtctg tctggtcgcc gttggtgacg
     3721 atgagcttgt tctcaaattt tctgattgcc gcatagatta tcagggaggg atcctctacc
     3781 tttgaagcgt cgaaaggctc ggtaaagacc tcaccgtttt ttaccgtgaa cacacggttg
     3841 cggctgtttg cgcttctgcc catgatgaag tatgccgttg cggctttttt gccgtcagag
     3901 cttttgccga taacgattcc gcgtcctaca tagggattgc cgtctatacg gtctttcatt
     3961 gatttgattt catatatgtt catttcaagc tgtcctttct ttaacgatct cagcggatac
     4021 aagctcggct gcctgaggaa gaagcttcca ttccgcctgc tccattactc ttttttgaag
     4081 aatttcggga gtgtcgccgt cctcgatatt gacggctttt tgaagaatta tttttcctcc
     4141 gtcgggtatc tcatttacat aatgcacggt tgcgcccgtg acctttactc cgtagtcgag
     4201 cgccgcctga tgcactttta atccgtagaa gccctcgccg cagaaggagg gaataagcga
     4261 cgggtggaca ttgattattc gctcgggata ttcagaaaca aaatctgcac cgagtatgct
     4321 cataaagcct gcaagaacga tcatgtcgat ctcgttttca cggagcaatt ccaaaatacg
     4381 gccttcaaaa tccgcctgat ttttgcactc tttttttatc acaacagcag tttttattcc
     4441 tgcgttttgc gctctcgtga gggcgtaagc cgagctgttg tttgaaacga caagtgcaag
     4501 ctcaccgcta tttattattc cgctctgctg agcatcaatg agtgcctgaa gatttgttcc
     4561 gccgcccgaa acaagaacgg ctattttagc cttgtttttc atctgtatgt actcctttta
     4621 gtaaaggtaa aacaattgcg ccaaacgtgt ttcctgcaac aacaagggct gtaaagagga
     4681 ttatttttac gctgaaaatt cccgaagcac ctaaataaaa catatcggca atcgaatgtt
     4741 caaagccgca gaggataaac acgggcacac aaaagagaat gcccagaacg gagtttttct
     4801 ccttataaat gctgactgca agatacatca gtattccgca gaaaaaaccg cggataaacg
     4861 tgtataacgg gaactgtgca agctttgccg aacacatttc ccccgccttt tcgccaatat
     4921 tcggcagaac cgtccgcagc agcattccga gaaaaaacgt tgcggcaaga ttgccgaaaa
     4981 gtccgatgtc gaggttaaga aaatcctgtt tcgtatgctt ttcgactatg tagccgattt
     5041 ttcccgtgaa aaggtagtag cttttatagc agatgcagag cagagcgacc gaaaagagca
     5101 ccgctccgac tgttttgttt t
//
LOCUS       DAWBSW010000147         5077 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2983_length_5077_cov_2.760852, whole genome shotgun sequence.
ACCESSION   DAWBSW010000147 DAWBSW010000000
VERSION     DAWBSW010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_5077_cov_2.760852"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1216 a         1293 c         1045 g         1523 t
ORIGIN      
        1 gctctgccgg cttcgatcgt gtattctctt aaatgtgaaa ggtcaaaatc attatttatc
       61 tcggtgcaaa ttctttcaag catctccgcc gaaagagttg tcggagtgcc gccgccccaa
      121 tagatagatt caagcctcag cccgagaccg tttgcgatct cggcagtctg ctttatctcc
      181 ttgcagagat tttcgacata atcgggcaag agcttctttg cctgtgcaat cgaatgggaa
      241 acaaaggagc aataattgca tcttgtcggg cagaacggta tggaaacata caggctgaag
      301 cttttctcgt cggaaagtga cattattttt tcctcggcct tggcaacgga ataggcaagc
      361 tcggttttat ttttgcaaac caggagctta tccgtgaaat acgcctttgc gccgttatca
      421 ccgtaatcct taataagcga attcatcagc tttgacggtc ttacacccgt cagtatgccc
      481 catttcggca catagccggt tatctcggaa agaagcgaaa acagcaaaat tgccatttct
      541 ctctcgcaat cgtcatagtc ggcaaccgtc tttttcttct cgcccgttgt tccgtttata
      601 ttgaccgaaa caccgacaac atattcgccg ctcattgaag taacaacggt tatatcgtcc
      661 gtttcatcgg actcgtcatt tatgacacgg atcgtctcat agggaaagaa aaccctgcaa
      721 aggttttcca tttcataatg aaatttgtga ttaacgaccc tgagtatcat ttaaatctcc
      781 tcatatatct gtttcttctg cgctcatatt ccatagtcgt ggctctgttg tgaccgggat
      841 aaaccgtata ttcaccgggc atttcataaa gccgcttgat tgaagcatac atatcatcgt
      901 cattgccgcc aaaaagatcg gttcttccgg tcgtaaggca gaaaagcgta tcgcctgtga
      961 acagcgtgcg ctcgctctcg atataatagc aaacactgcc cattgtgtgt ccgggggtat
     1021 gcatgacctt aaattcaagg cttccgagag aaataacatc tccctcctcc accgtgatat
     1081 ccggctcgat atttttctgc atatgcggtg caatgctgaa tgcaatactc atcatctcgt
     1141 ttgtaagaca atcggcatcc tttttatgaa tcacgatttt ggcctcggga aaagcctgtt
     1201 tgaggtcata tacgccgaga atatgatccg catgaccgtg ggtcagcagg atatatttaa
     1261 catttttgcc gttgagtttg ctgataagct caggcgtaca ttcgccacag tcgattatcg
     1321 ccgtttcgca gctaatatcg tccgttaaaa tatatgtgtt tgcgctgaca ggtcccagca
     1381 ccattgtttc aacagtcatt ttaatccctc ttgttccaga tagtcgtgtc cataattatc
     1441 gtaacggggc cgtcgttgtg aatttcgacc ttcatatccg ctccgaatga accgtgctcg
     1501 acctttttaa ttccgtttac cttgagctgc tccatgaaat attcataaag ctcattggca
     1561 tagtcgggct tcgccgccgt tgtaaaagaa ggtctgttgc cctttttgat atctgcgcag
     1621 agcgtgaact gagagattgc aagaacctcg ccgtcaacat cgagaattga catattcatc
     1681 ttctcatcct cgtcctcaaa aatacgcaat ttggctatct ttgcggcaag aatttccgca
     1741 tccttctttg tatcgtcggg gaaaacgccc aaaagaacat tatatccctt gccgattttt
     1801 cctatcgttt cgccgtcaac gactaccgcc gacgaagtta ctctttgtac tactgctttc
     1861 atatcaaaac ctcttatttt ttaccttagc tgttggaacg ttcaacatca agaacaccgt
     1921 ttatctttct gagcttcgcc ataacaccgt tgagatgctc gattccgttg acgccgattg
     1981 tcaattcgag cgatgctctg ccgtcctttt tgctctttgt attgattccg tatatcataa
     2041 ctctcatgtc tgcgatcatc tttgaaacat cagccataag tccgactctg tcaagacaag
     2101 tcatcgaaac cgtagctgtg aaatcctcct taacagagct gtcccatcct gcgggaatcc
     2161 atctgtcagg ctcatcggca agctcgataa ccttaggcac gtttgtacag tcccttttgt
     2221 gtatggaaac gccgtgacct cttgtgataa aaccgataat cttatcgccc ggaacagggc
     2281 tgcagcactt tgaaagcttg ataaggcaat tgtctattcc ctcgataaca acgccgtcct
     2341 gactctttgc gcgcttcttc tgagaaggct ttgttatctt aataacgtcc tcctcagccg
     2401 gctcggtctg cttgaactcc ttaacgtact cgtccttgat atgcggcatc atcttgttaa
     2461 tggaaacgcc gccgtagccg attgccgcaa agaaatcatc ggcactgttg aagcgctgac
     2521 gctgagcgac ctcaaggatg aacttgtcaa actgctgatc gttgagcctt atgaaattgc
     2581 gcttgaactc acgctcaacc tccatcttgc cctcaaggat attttcctct ctgcgttcct
     2641 tcttaaacca tgagcggatt ttattccttg caccgctggt cttgacgaat ttaagccaat
     2701 cccggctcgg tcccttattc tgctgagagg aagtaatgat ttcaacaatc tctcccgttt
     2761 tgacaacata atctatcggt acgattcttc cgtcaacctt tgcgccgacc attctgttgc
     2821 cgactgcaga gtgtatggca taggcaaagt caacaacggt tgcacctgcc gggaggttga
     2881 taaccgttcc cttgggtgtg aacacaaaaa cctcctcctg tacaaggtcg gtttttatcg
     2941 tgttcacaat atcctgcgga ttctcggaaa cattctggct ttcaagaagc tgacgaatcc
     3001 atgaaaattt ttcttcaaac ttctgtcttc cgctcattcc gagcttatat ttccagtggg
     3061 cggcaacacc gtactcggca gtgcggtgca tctcccatgt tcttatctga acctcaaacg
     3121 gtattccctc gtcgccgatt accgtcgtgt gcagggactg atacatgttc gccttcggcg
     3181 ttgaaatata atccttgaag cgtccgggaa tggacttgaa catatcgtga attattccga
     3241 gacagttgta gcagtcaata acgtcatcaa caattattct gacggcataa atatcgtaga
     3301 tctcatcaaa gcttttgccc tgcatataca tcttacggta aatgccgtga acactcttga
     3361 ttctgccgga aatgcttgcg ttcggcacaa caagctttat tctctcgctg atttttttct
     3421 gaatactgtc aaggtattcc ttcctctttg actgctgctg ttcgagcctg tctctgatct
     3481 ccttataagc aacgggatca agatagctga ttgcaagatc ctcaagctcc tccttaacgc
     3541 ctctgatacc gagacggtga gcgatcggag catatatttc aagtgtttcc tgagcttttt
     3601 cacgtctttt atccggtcgc ataaaagaca aggttctcat attgtgtata cggtcggcaa
     3661 gcttgatgat gataacacga atatcctgcg acattgccat cagcatcttg cgcacattct
     3721 ccgcctgctg ctcctccttt gtaaagatag gcaccttgcc gagctttgta acgccctcaa
     3781 caaggtttgc aacatcctcg ccgaattcct cctggatatt ctccttggtc atatcggtgt
     3841 cttcaacaac gtcatgcagc aaagccgcag ccattgactc cccgtccatg cccaaattgt
     3901 aaagaataat tgcgacggag acagggtgga ttatataagg ctcgccggag aatctgaact
     3961 gaccctcatg tgcctttttt gctataaaat acgccttttc gacgagggaa acatcgtttt
     4021 cggtcatgtt ttccctgaca agctttataa gcttgccgta aagttcatcg ggattttcgg
     4081 ggacgaaatc tttcttttcg tcctgctccg cagtcgcttc gttcatcgtt gcggttaatt
     4141 tgtttttttc attagcttct gacattttta taccactcgg cttaccatgc cgattccttt
     4201 aaaagttctg ttaaggcaat tcgcactctc cggtcgtgag gtgttgcctt aatcttttga
     4261 gcaccggcgc atcctgcaag ttgaccttct cgcttgtttt cggtgcgctc agtccgccgt
     4321 tttcatctct tatgagtata cccatttcaa gcattgcctc aacggacacc gctacctgtc
     4381 cgtaatcctc cgcccagaaa tcaagcctgt gacagagcgt ttccgtatcg taattccatc
     4441 ttccgacgga tcgtatgtat ctgtaaacct tagccgttgt ttcacgctga ggaacaagca
     4501 gcgaagcgtc gaagccctcg ctttttcctc tcatgagcat tgaaaaatct ctcatcgaaa
     4561 caaacagatc gtcctccacc atgtcgtgga gctttatatt tttgagcaaa acgctcaccc
     4621 taagctggcc aagatattca ttcggttcaa gtccgacagc agcatcaatc acatcaccct
     4681 tgacgtacgg aaaatccgcc tgaagcgttc tgaattggag cgtttgtaaa gaaattccgt
     4741 ttcgttcaag gattattctc aggtgctttc cgtttcctat cggttgaata tcggttatcg
     4801 tcatgttgta aagaccgaaa accggctgag ggttgcctgc gccgaatggt tcaagctgtt
     4861 caatcgcgct gagcatttcc acattcacat atgcagggtt cagtttaaaa tcaacagtct
     4921 gaattgggaa aggcatttcg aaactgtcgg catattcgtt tatcgctttt ctgaaagtgc
     4981 cgatgttttc tgttttaagt cctattcccg cggcaagcat atgtccgccg aaatgagtca
     5041 gacactcgcc gcaatgtgaa agtgcatcgt aaagatt
//
LOCUS       DAWBSW010000148         5066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2992_length_5066_cov_2.365596, whole genome shotgun sequence.
ACCESSION   DAWBSW010000148 DAWBSW010000000
VERSION     DAWBSW010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2992_length_5066_cov_2.365596"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1484 a         1217 c         1160 g         1205 t
ORIGIN      
        1 gcataggagt catcaatctc atgggcagtg cttttttcaa tgcaaactat gccaatccgt
       61 ttgacagtct cgacagggcg attgaggaaa tcaaggattg caaaataaaa atcgttgatt
      121 ttcatgccga agctacggga gaaaaaagag ctctcggctt ctatgccgac ggacgcgttt
      181 ccgccttttt cggcacccat acccatgtca gaaccgccga cgctcagata cttccgcagg
      241 gcacaggtta tataaccgac ctcggaatgt gcggcagtga agattccgta ctcgggattg
      301 agacggattg cataatccgt gctctcaaaa cgggtatgcc gacgcttttt caagctaagg
      361 aggataaaat tcttttaaac ggctgtatat ttgaaatcga cgaagcaacg ggaaagaccg
      421 tttcggttga agcaatagat aaaagggagt aataccatgt ttaaatacac caaaagaata
      481 atcgccgctt ttgtatgcct gacggttctt ttctcgctct gcggctgcgg cggtaaatcc
      541 gtaattgaca caatttcaaa tctcggaaag gataaggagc ccgagcctat tgacgagatc
      601 ggcttcaccg ttccttatat acgcacggat tctcttgacc cctataagct gacaaattcc
      661 atgaataaat acatttcgag tctgatttat gattcgcttt tcaaggttga cgagaccttt
      721 aaagagaccg ccgtaatcgc cgagtcatac accgttgcgg acaaaaagct caccgtaaaa
      781 atcaagaacg gtctcaaatt cacggacggc acacccctca cggcaaacga tgttgtctat
      841 tcattcaatc aggcaaaagc gagcgtaaca tacgccgcct atcttgaaaa catagactcg
      901 gcgtcaaccg acagcactta taccgctgct ttcacgctca ggaataccga caaatccgag
      961 gcggcaaacc ttatttttcc gattgtcaaa aaaggttcct acgacaaaac agcaagcgag
     1021 gatatacaaa ccgacagcga cgatgaagaa agcacaacga cggcttccgg cggcggatat
     1081 acatcaaaaa ttcccgtcgg cagcggacgc tatacctttg tcactaacag cgaaagcaaa
     1141 tatcttcaat ataataaatc ccgtctgggc ggatacaatc cgaaatatgc aaaaatcggt
     1201 ctcattgata tttccgcatc cgaatctttc agcagccttt tcgagctgaa taaaattgac
     1261 ttttactgcg ataattttga tagcggaaaa tacaccaagt ttacaaaaat aagcaacaat
     1321 attgagctta ccaatcttgt atatctcggc atcaattctc aagacagcgt tctttccgag
     1381 ccaaaggtcc gccgtgcaat ttctcttgct ctcaacagag ccgaacttgc ctccgacgcc
     1441 tttgcgggct gcgccgtcgc ttcggctctc cccttccacc cgtcatatta caagctcaag
     1501 ggctgcactc tgccgacttt aaatcaaaaa acggacagtg caattgagct tcttgaggac
     1561 gtaggatatt caaaaatcaa cgattccggt gcaagatata acgagaataa agtcatggat
     1621 ctgaccctgc ttgtaaacag cgaaaatgat tttcgccgtt ctcttgcacg cggaattcag
     1681 caagcgctcg aaaagatcgg catcaatgtc actatcagag aagcctccta ttccgactac
     1741 gcttcttcga tatcaagtga cagcttcgat atgtatatcg gagaaacaga gcttccgaac
     1801 agcttcggtc tctcgagatt tttctcgacc gacggcggac tcagatacgg tatttccgaa
     1861 aaaagcgaaa cggcaagaat ttataacaaa tacctcggcg gacaggcgga tatgcaaaag
     1921 cttatcggca cgttttccga cgagcttccg tttatcccca ttgctttcag acagggcatc
     1981 accgtcagct ctgataaaat aacgagcgaa atcaagacag ttcccggcga ctgctttgcc
     2041 aatatcgacg aatggacggc gcagtgatgg acagaaccac caatatacaa tatttaaagg
     2101 gagtcggtga aaaaagagcc aagcttttag ggaagctcgg gattttcacc gtcggcgatc
     2161 ttctgcgcta ttatccgaga gaatacacgg actggagcaa ggtgacgagc atcgccgccg
     2221 ctccgtttga cgagccgtgc tgtgttaaag cgaccgttga ccacaagccg aagggagcca
     2281 aggtcagcaa gggaatgacg atttacaaaa ccgttgtgac cgacggcgag tcgcttatga
     2341 acatcacgat tttcaacagc aagtacaccg ccgaagccct cgaagcgggc gaagaatatc
     2401 tcttctacgg aaaagtcggc ggaaatttcc accgacgcga aatgtcctca ccgctgattg
     2461 aaaaagcttc aagcggtgaa aggattcatt caatatacca cctgacggca ggcgtcaatt
     2521 caaaatatat cgaaaagctt atacgtcagg tattttcctc cggcaacgat tattttacag
     2581 attgtctgcc gcagtcgctc agagataagc tctgcctgat ggagattaac aaggcaattc
     2641 acgagcttca cctgccgaca aacgaggata tgcttcacga ggcacggcga agaattattt
     2701 ttgaggagct ttttattttc cagctcggct taatgaagct gaagggtaac aggaaagaaa
     2761 caacaccggt tgccgtcaca caggattata ccgacgaatt taaacgtctt ttgcctttca
     2821 ggcttacggg agcgcagaac cgtgccgtca gcgaaagtat aagtcagatg acaagcggga
     2881 taacgatgaa ccgccttttg cagggcgatg tcggctcagg caagactgcc gtcgccgccg
     2941 cactgattta taccgttgtc aagaacggat accaatgcgc attcatggcg ccgacggaaa
     3001 ttctcgctca acagcatttc ataacatgcc aaaacttttt taaagataca aacattcatg
     3061 ccgagctgct gacaggctcg gtcacggcag caaacaaacg ggcaatcaaa gagcgcctga
     3121 aaagcggtga aacccggctt ataatcggca cccatgcgct gattcaggac gatgtcgagt
     3181 tcagtgctct cggtcttgtc ataacagacg aacagcaccg cttcggcgtt aagcacagag
     3241 cggcactgag caaaaaagga gacaagccgc atacacttgt catgtcggca acacccattc
     3301 cgaggacgct tgccctcatg ctctacggcg atttggatgt ctccgtgctc gacgaacttc
     3361 cgccgggcag acagccgatt gagacatatt acgtcacagg taaactccgc gcaagagcct
     3421 tcggctatgt caagaagcac ctcgatgagg gtcggcaagg ctatatcgtg tgtccccttg
     3481 ttgaggaggg tgacgaggat atcgccgccg ccgagagcta tgcggataat ctttcaaaag
     3541 gattttttaa cggctatgag gtcggactgc tccacggaaa gatgaagtcg aagcaaaagg
     3601 acgaggtaat gtcacgcttt gtttcgggtg aggtcaagct gctcattgcc acaacggtca
     3661 tcgaggtcgg cgttgatgtg ccgaatgccg tcattatggt tatcgaaaac gccgaacggt
     3721 tcggtctttc gcagcttcat cagctcagag gacgaatcgg cagagggcag tataaatcaa
     3781 cctgtattct tatctcagac gcaaaaaccg acagcgcaaa aaagcgcatg gagataatga
     3841 caaaaacgac cgacggcttt aagattgccg acaaggactt gaaaatgcga ggaccgggcg
     3901 atttcttcgg agcacgtcag cacggactgc cgaacatgaa aatggcatcg ctgaccgact
     3961 cggcattatt aaccgaggct caccgctttg cgcaggagat tttgtcagac gacctcaatc
     4021 ttcaaaaggc cgagaacagg aacctgaatg ccgcaattta tgagctgttc aattcggaat
     4081 atataatgaa ctgacaggat taaatcttgc ttgctgctag gtgccgatgt gtaaaaaatt
     4141 caccgaaacc tattccgata aataatgata aaacggaact tatgcataaa tatgcgttgg
     4201 ttccgtttgt tttaagtatt cgctgataaa aacaaagccg atttggtgaa aaaatatgca
     4261 aaaaatcgtt ctgttaacag tttatttgtt tttcaattct tgactttata atacataata
     4321 tagtataata atctgaaaat atattggtgg tgaagctatg aattcgtata tcaatcccct
     4381 tacaaatcaa actacacagt ctttggttga agaacttaac aaaagctatc agataaatcc
     4441 cgattatttc aatcgtttca atgttaaaag aggcctgcgt aattccgacg gaacgggcgt
     4501 catggcaggc attacaaaaa tcagctctgt tgacggctac tacatagatg acggtgaaag
     4561 agtacctaag gaaggtcatc tctatttccg cggctttgac atgcaggaaa tagtaaagca
     4621 ttgccgtgag gaaaaccgct tcggatttga ggaggtcgca tggctgctca ttttcggctc
     4681 tcttccgaca accgagcagc tcacacgctt caaggatatt ctcgccgtaa gccgtaatct
     4741 tcccgaggat ttcatcgagg acacgatgat gaaagctccc tcgccgaaca ttatgaataa
     4801 aatcacgaga acgattcttg cactttattc atatgacgag tcgcccgatg atacttcgct
     4861 tgaaaacgtt gtacgtcaat cgattcagct tgtcggtcag ctccccgtta tcatggctta
     4921 tgcctatcag gtaaagcgac gtcattatct caaaaagagt atgtatatcc atccggcgaa
     4981 gcccgaccag tcaacggccg aaacaattct ccgttcgatt cgctcggaca agactttcac
     5041 ggatgacgag gcgaagcttc ttgacc
//
LOCUS       DAWBSW010000149         5061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2995_length_5061_cov_2.103476, whole genome shotgun sequence.
ACCESSION   DAWBSW010000149 DAWBSW010000000
VERSION     DAWBSW010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2995_length_5061_cov_2.103476"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4613..4622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1468 a         1080 c         1192 g         1311 t
ORIGIN      
        1 gatttgcaat gattgcggat ataaattcag aaacacagaa gattggcacg aagaaataaa
       61 gaaaaaagag caagctgtga aagtcaacct tgtgttaaca atctttttta ttatcgttgc
      121 tatgttactg tttacaggtg gtgaagtatc ctcagttgtt ggcattgtgt tcttgattat
      181 tggagcggta aatgctatca cttatttcct aggaaaaaca aggattcgaa atgaacttca
      241 acatcttgat gatctcgaaa gttcctctat ggagtaattc agtaagcaaa agatttttaa
      301 aaacagttgg gaaaacccaa aaaactgcga tttgataaac aaagaataag gagtgtgaca
      361 aacatgggca gtttatttca ggatatgcta ggcagcgacg gtcaaatgat cgatacaaat
      421 gttgatatcg tttttgtgat cgatgccacc gaaagcatgc agccgttaac tataagttta
      481 caaaaaattt acattttgaa ataagctttg ggtaatcaag gagaatttta tatgaagaaa
      541 aacaacaaac cggtcaggga atactatact atcgacttag tacatatttt taaaaccctt
      601 tggcagagaa tatggctgat tgtcttggca ggcataattg ccgcaggaat cggattttcc
      661 gtgtcggcat ttttaatcaa gccggagtat tcctcatcaa taatgcttta tgtcaacaac
      721 agctcgtttt cgttgggcaa cacgagcttc agcatcagct cctccgaaat caccgccgca
      781 cagagccttg ttaaaacata cggcgagatt ctcaacaacc gctcgaccct tgagcgtgtt
      841 attgaaaagg ccgatgttcc gtattcgtgt aagcagttga gcaaaaaaat tcagtccggc
      901 cagtcgaaca ataccgaaat catgagggtt gacgttacaa ccaacgaccc ttacgaggcc
      961 gcaaagattg caaactgtat cgcagaggtt cttcccgtaa gaatatcgga gattattgac
     1021 ggcgcctcca tggaggttgt cgatgccgca gtgccgaacc ttgaaaaggt ttcaccgagc
     1081 ataactcaat ataccgccgt cggtctgatt ttgggagtgt tgctttcggc attggcgatt
     1141 gccgtcggag ccttgatgga tgacactatc cacgacgaag agtacatctt acagacttat
     1201 gactacccga ttttggcgaa ggttcccgat cttttaggct cggacaagaa aaagtacggc
     1261 tactacagcg cccaaaagaa aacctcgaag aattcataag gacggtatat agacatggga
     1321 ttatttaaaa ataataaaac aagcgctgtt tttaccgaaa aggattcaag aaagactctg
     1381 cacaagaacc ttgaatttac agccattgaa cagtataaaa ttctcagaac aaaccttgat
     1441 tttaccctgc ccgaggacgt taagtgtccg attatcggcg taacaagctc gatgagagga
     1501 gagggaaaaa gcacgaccgc agtcaatctt tcatatgttc tcgccgaaaa gggcgatccg
     1561 gttattctta ttgacggcga cctgagaatt ccttcaatcg caaagaaaat gaacatcgaa
     1621 tccacaccgg gtcttaccga ccttttgatg ggtcacggtg ctcatatgcc ggatttccgt
     1681 tcggcattgc tgaagaactg gtacatattg ccctcgggag aaattccgcc gaacccgtcg
     1741 gagcttctcg gctctcgcag aatggaattt atcctcgaca gccttcgtga aagcttcaat
     1801 tatatcatca ttgatttgcc gcccgtcaac atcgtttcgg acgcattgtc gatagcctca
     1861 cttatcgacg gtatggtagt cgtcgttcgt gaggactaca ccgaaaagaa ggaattggag
     1921 cgttgcttca gacagcttca gctttctaac gtcaatgttc tcggctgcat tttgaacgaa
     1981 tcgaagaacg acggcggatc ttacaagaaa tacagaaagc acaagtatta taaatactac
     2041 aaatactatc aaggttcctc gaagagtgat agaaaatgat tgattggcat tctcatattt
     2101 tgcccggcat cgacgacgga agcggcagta tcgaagaaag ccttgcgctt ttgaaaatgc
     2161 agcgtgaaca gggagcggat accgtaatcg caactcctca ttttttcgcc gacgatgaaa
     2221 cggttgatga attcttagcc cgcaggcagg aaagtctcga cagtctgctt tcagcggctc
     2281 gggacgaaat tccccgtata atctgcggtg ccgaggtcaa atattatccg ggtatcagaa
     2341 aattggagtc gttgagcaag ctctgcatcg gtgaaagcaa tctgattttg cttgaaatgc
     2401 cgatgggtaa atggacggaa tatacggttc gtgaattgac cgaaattgcc gtgactcaca
     2461 gggtgagggt aattctggct cacatcgaca gatacttaaa gcttgaaagc gattccgtgt
     2521 gggaacgtct ttacgaaagc ggaatcctga tgcaggtgaa tgcatccttc ttcaatgagc
     2581 ttttcacaaa gaaaaaggcg acggatatgc tcgctcgggg cggcattcat gcgatcggct
     2641 ccgattgtca caaccttcgc aaccgtccgc cacgcttggg taaggcatat gagctcattc
     2701 gcaaaaagct cggcgatgag ttcgttacac agtttaatga gtatggttat tcattactct
     2761 ttgaataaaa atttatttga aaggattttt attatgaaaa aattatcaac ctttttaact
     2821 gcaattttaa tagcgtcaat ccttacggta ggcgtatttg ccgcaccgtt catcaaaagc
     2881 ccgggttccg catccgctcc cgagctcatt gaatacgaat cggaatctcc ggagtgcaag
     2941 gcgagactgg ttatcacacc gtattctcac cgtgacgatc tgaacaacga cctcaagtcg
     3001 atgatagtaa aagcgtataa cgaaatccgt tccgcaggtg atctcaccga gttgaacaag
     3061 gatctggcaa ccgtcgctca gagtaagggc atcgcaacca ggaatttggc agtctgcgat
     3121 ttgttcgatc ttcactatga gaactgcgcc caccatgagc cgcacggcag cttcagaatc
     3181 aagctcaagg acgacaacgc aaacagattt gttgcgctcc tgcactatta caacggcgag
     3241 tgggagctga ttgacaacgc aaaaatcaac ggcgaatatc ttgaatttac catcaaggag
     3301 ttctctcctt ttgcaattgt tgtcgataat tccgatgttg ccgacgatac gggcacagcg
     3361 gaaaatcccg caacgggtaa catcgaggac ggaatcaaaa tcggcgtatt ggcaggagta
     3421 atgtgcgttt ccctggttgc cggtgttgtc ctttggaaga aatctaagaa acaggccgct
     3481 taaaaagagg agtctttatg ggaaaaaaac ggaacgataa aagaaagctg atgcgtggct
     3541 tagccgtcgc tcttttggca gtgtttcttt taacaggtgc ttttttgctt ttggaattat
     3601 gggaaaagcg tcaaagcatt ttccccgagc aaaaaaccga gaataccgtt tatgaatata
     3661 acggtgtcga gtatgtgaaa aacgaagatg ttgaatcgtt tttgatcctc ggccttgata
     3721 agtttgagga cgcaattaat aacgattctt acaataacga tcagcgggcg gacttcttga
     3781 tgctgttggt ctttgacaac agtgagaaaa agttcacggc ggttcacctt aaccgtgaca
     3841 caatggttaa tatgaacgtt ttgggcgttg ccggtcagaa gattggcacg gtcaacaaac
     3901 agctcgcctt ggctcatacc tacggcaacg gcagggatgt cagctgtcgc aacaccgccg
     3961 acgctgtttc cgagcttttg aacggcgtta aggtaaatca ctacctttca ataaccatgg
     4021 acgccgtgcc gattttgaac gaccttttgg gcggcgttga ggtcaccgtt ttggacgatt
     4081 tcagcggtat tgacgacaca ctcataaagg gtgaaacggt tacgcttcac ggcgatcatg
     4141 cgctcaccta tgtgcgcgaa cgttacggac ttgaggacag ctcaaacagc acccgtatgg
     4201 tgcgtcaaag acagtatatg accgcagttt acgataaggc tatgctcaaa atcgaaaacg
     4261 atgacaattt tgttattgaa gcatcttcaa agcttgccga ttatatcgtg tccgatcgct
     4321 cggtcaatca gcttcaggaa attgcgaaaa agctgtcgca gtataaattt accgaaatcg
     4381 aaacccttga gggcgagtcg gtcgtcaagg acggcttgat ggagtttcgc cccgacgccg
     4441 attcgattga taagattgtt tttgaattgt tttacaagaa gaagtaaaaa gaacaggagg
     4501 agcgtatgga aggcaagaac aattatatca tttcctccgg ctcacaaagc gaaagcgccg
     4561 agcccgaaat tacaatatat gaaaagcctg tctatctcgc agtcaaaaga tgnnnnnnnn
     4621 nntgtataat tttttttaca attccgattc tgtttgtaat gctcttgatc gttatcgaca
     4681 gtccgggagc gtctcctatt tatacccaat acagagttgg caaaaacggc agggtattca
     4741 agttttataa attacgttca atggtgcccg aagcggatca aatgttggat tcgttgcttg
     4801 atcaaaatga aatggaaggt ccggcattca agatttcgaa cgatccccgt atcacacggg
     4861 tcggtcgggt gatcagaaga acctgtattg acgaattgcc tcagctttgg aatatcctaa
     4921 agggtgatat gagcttcgtc ggcccacgtc cgccgcttcc gagggaagtt gagcagtaca
     4981 acgattttca gcggaaccgc ttgagcgttg tgccggggct cacctgttat tggcaaattc
     5041 agccaaacag aaacagcctg a
//
LOCUS       DAWBSW010000150         5049 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_2999_length_5049_cov_3.052663, whole genome shotgun sequence.
ACCESSION   DAWBSW010000150 DAWBSW010000000
VERSION     DAWBSW010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2999_length_5049_cov_3.052663"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1398 a         1189 c         1002 g         1460 t
ORIGIN      
        1 tcaattccga gaacttttgc cgtttccgcg acagcgttgt gcttggcata attccagttt
       61 ttcgtgctcg gaacgctgac gagcacaagc tcagcattat tctttttaca atagtcacgg
      121 agcttggtaa tatattttac attgtccttt gaaattgttt ctttactatc gtcgggcttc
      181 atatattcat acgcatccgc aggactgacc gacatattaa aaagatagcc cttgcttcgt
      241 ctgtcatagg ttttagcctc gttaaatctc cagcttttcg gctgcataag cttccatcgg
      301 tcgtgatact taaacactga aaacaagccc tcagccttat tgagaaacgc ctttgtactt
      361 gtaacctcac gaaaaatcgc atttgtttca agcacaacaa ccttagggtc ttgtttcttc
      421 atgctcttaa taagaagctc atacgaaaag ctgagcacct gatccgatgt gccgcagaca
      481 tatgaggtta tcccatgctc cttccagatt ttcagcggta taaacgtgct gtagcactcg
      541 ctgtccccga gaaaaaccac gtcaatcgta ttcttttttt cggaaagaat tccgtttgtc
      601 agcgggtctt caagcgcatt gacctttgcc gatttgccgg gcttcataat gaaagaaaag
      661 cccatcagca atgaggcgag aattgcaata aaaagaaccg cacttattgt atttttcaaa
      721 atcttattca taattatcct ttaatgcaat gacgcacggt tgaggtgtgt gtttttttct
      781 tagaaattag cgtaaatcgg gtcaacgggc atatagccga ttccgtaggc gccgaaaatc
      841 acgatataca tgcacagcgc aagcaaaacc gcccatcgca gtacgatatt tttcttttta
      901 agctctgctc tgacgcatat tcccctctcg ttgagaacgc tgacaacgaa cacgattgcg
      961 acagttacgc cgacaatcag caaatccgca tagtcaacac cgagctttgt gaaaagagta
     1021 cgagccgacg agccgaaact gaaatccgta accattctgc cgaacatttt catgcctgct
     1081 ctcagtccgt ttgcacggaa gaaaagctca ccgatgacaa caaaaatatc gcttctgata
     1141 atctgcattg ccatatacgg cttcgacttg gtgttgacat gaagcttgcc gttgaccgtg
     1201 ctgatcgccg gcgcaagaac tctgccggtc agtatcagcg caaaatgata cattccgaaa
     1261 aaaatatagc tccatgcggc gccgtgccaa agtccgtttg caaaccaaac gcagaaaagc
     1321 gcaacacttc ccgcaagcaa cggaccgaaa tgattgccga gacttttcct tgccgacttt
     1381 gttagctttt gcatcggctt tgacatcgtg atcggataaa agatataatc cttaagccat
     1441 tcgccgagcg taatatgcca gcgtttccag aattcggaaa tcgtttttga aaagaacgga
     1501 cgcttaaaat tctccggcat ttcaatgccg aaaatctgtg ccgtgccaat aactgcgtcc
     1561 attgaacccg aaaagtccat atagagctga attgtatagc aaacagccgc aaaagcgata
     1621 acgccgccgt gatactcact gtaattatta aaaacattct ccacaggcag gttaaggcgg
     1681 tcggcaacga ccatcttttt catcataccg aaaatgatac gctgaagtcc gagcgtcaat
     1741 gaatcaaatt tgactctttc cgctttccaa agctgctgcg ctgtctgaga atagcggcaa
     1801 atcggtcctt ctacaatctg agggaaaaac gaaagccaaa gaaaaacgcg aaagaaattt
     1861 ctgtctgcct gattttttcc tctgtagaca tcgatgagat acgaaaccgc ctgcaaagta
     1921 aagaatgata tgcctatcgg cataataaac ctcggtatac tcattctcac tccgacattc
     1981 atcaaggcaa gaagcgaatt gatattgcct gctgcaaagg ccgaatactt cagcacaagc
     2041 agagcgccga tatggataac cgtcgcgagg gcgagaacaa gacgtaaacg cccggtaaat
     2101 tgtttcttta tctttttctt ttcttcacgg tcaagcccat gaagagcttc cttctttttt
     2161 aagttgattc tatccagcca aagtccaaag ccgtaaattg aagcggcgga aatgataaga
     2221 tagacaatca attttccgct gatgagccaa aagaaggcac agctttcaaa aataagaacg
     2281 tatcttcgga ttttctgcgg cgagagccaa taaagagcaa tcgcaatcgg aagaaagacg
     2341 aacatatatt caatgctgaa aaaagacgtc attcccgagg ctccgccggg cataagaaac
     2401 gatgaaagtg acatatcggc tcacctcatt tccgatcatc gccgagaaaa aatattctgt
     2461 tttttgaagc gatgcgattt gtcattttgc tgtcctttta aattatgatt ttgaaagcgg
     2521 taatcttatt tcaaatgccg cgccaacctc attgtaagcc gttattgtgc cgttatgcag
     2581 gttgacaatc tcacgtgaga gcgcaaggcc gatacccgtg ctgccctttg ttcccgtata
     2641 gaaacggtca aagatatgcg gcaggtcatc ctcggcaatt ccgttgccgt cgtcgctgat
     2701 ttttaccata gcggatttct gctcggcagt gcataaaact acgattgcac tgttgcaatg
     2761 ccgaattccg ttcgtcagaa catttatgaa tacccgtgaa agctgatttt cgtcgcagtg
     2821 aacccatacg ggactttcgg ggaacatcgg agttattgtg attttcttct tattctgaat
     2881 cggctctaca gaacggatac aatcatatag tatttctctg aggtctgagg tcgtcatatt
     2941 aagcgggagc tgatttgcgt ctatctttga gattgaaagc atctcgtcga tcatatgagc
     3001 cattttgtcg ctctcacgca tgataatctc cgccgaggac ggaatatcga caacgcctgc
     3061 ttcaattccc tcggcatagc cctgaatagc cataagcggc gtttttaact cgtgcgaaga
     3121 attctggaag aattcatgat tggcttcgtg ataatcctcg atcctgctgc cgagcttata
     3181 gccgataacg ctcataaccg cgatcacggc gacaaatatc gcagcaaaaa cccagttgag
     3241 cgatcgaaca taattcacaa gcggctgaat gtttatatac attaccgttg attgataaga
     3301 gccgtccatc gtaaatgtat cgtgatactt tacaagcaca taataaccct tatcggttat
     3361 cagcttcctg atatcgccct ctttaagcga gctgagactg taatgcgatc ttatctcgtt
     3421 ctcggaattc tgcgcggagg aatttgattc tgaacccttt atcggagttc cgctgaattc
     3481 atagccttcg tcaaagaaat ttataaaatt gatgttgccg ctgagaaaag agccttcata
     3541 ttcatcgtcg gcatagtcat tgttttcaaa cttatctata tattccatct cgtattccag
     3601 cgcatttatc gcttcctttt caaaatgaga gggaacaatg aggttgacta tccctacaaa
     3661 gcccgaaaaa ataacgataa ccgaaatcca aattatgcaa agtattttca gtttaacatg
     3721 tttcatttct tatcaccgtt aacctcaatg cgatatccga agccccacat tgttctgacg
     3781 ctgacattgc tctcacaggc cgaaagctgt tttcttatgc gtctgagcgt ctcgtcggta
     3841 actcttgttt cgaccttggt atcatatccc catattgcat tgagaagctc atccctcgaa
     3901 tatgcctttt cgggattttt catcatatat gaaagcattt tcagctcggt tcgtgtaagc
     3961 ttgacgggtc tgccgccgca aaggatttca tttctgctgc ctgaaaaggt caaatcacct
     4021 atacttatgt cctcatcgga catttttgag ctgcgatcgc ccttttcgtt catatccata
     4081 cggcgaagca gtgaacgtac gcgcatgaga agcgctgtcg gtctgaacgg cttggtgaga
     4141 taatcatcac tgcctatggt tattccctga acataatcat attccgaatc cttggcggtg
     4201 agaataataa ttggaacact cgatttctca cgcagctttc tgcacagagt caatccgtcc
     4261 gtgcccggca tcataatgtc aagaattacc aggtcggcag gtttttcatc aaagaccgcc
     4321 ttgagttcat cgcccgtttc aaatgcggaa acctcatatc cgtcgctttc cagaaagctt
     4381 ttgataaggt ctcggatatt cttctcatcg tcggcaatat aaattgaatg gctcatatgc
     4441 ctcccccctt ttcattttac agtttaatta tatatctttt ctttttggct ttcaccgaaa
     4501 aacaacgtca aatatgtcac aaaagtgtca tatacgatta tatcacacgg aacgatttct
     4561 cgcaacatat tttaaatttg gcaggtgagg aaattatttc atcgttcttt tctttatccc
     4621 ttcggaaaga tagttttcag attaaatttt ccgtcttttc gtagtagcaa ccatcggctg
     4681 tccgattttt tcttctgaat tcaaaggacg tgcacgcccc tactgactat gtctaagttt
     4741 cttctgacct tgggtcgatg cggtcatcga ctcctactga atgtgccttt atcgaacatt
     4801 tttgaatccc gccttttttt gccttctgcc accagaatta taaattacca aaaaatcaaa
     4861 aaattttata aaaaaggatt gacaaattaa atctgctgtg ctattatatc gtcaacctac
     4921 caaacgggta gttaattttg aaaggagaat tgcatcatgg cgacattgtt taacgcaacc
     4981 tataccaccg gaatgtgcat gtgtatgcgc acttattgtc atgccctttt tcgatgcgtt
     5041 ctcaattca
//
LOCUS       DAWBSW010000151         5006 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3020_length_5006_cov_2.515653, whole genome shotgun sequence.
ACCESSION   DAWBSW010000151 DAWBSW010000000
VERSION     DAWBSW010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3020_length_5006_cov_2.515653"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3262..3294
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3979..4089
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1140 a         1318 c         1065 g         1339 t
ORIGIN      
        1 ataacaatca tagctatcgc aagtattgca gcgaacaagt atgcgcacca aaccgatctt
       61 gcataattcc tgcgattgat gtttccgtca ctgaagcaga aaacgaggtt aactatcaga
      121 cctatgacag gaatagaaaa caaaatcgaa tagccgaaat atgcccatgc tcctaacggt
      181 ctgtactcct ccggacccgt gtagtttttc tttgccattt ttatcatcct tttctttttt
      241 tattatatca ttccgaaagt aagttttcta tcggactgat atcaagcttg tcaaactgct
      301 ttatgaactc gtaaaagctc actgcgattt tctttactcc gcccttttcg ttgctctcca
      361 tattaaacgg cataacgggg tcgtgcacac tcagacgaag aaggagccag ccgtttccgt
      421 tgtccttatc ggcggaaatt ctcatgccct cacggttgtc gtcggcaatc gtccagccgt
      481 ccttgcccga ataatacttt tcaagctcct cgattatcct ctcgccgtat gctctgaaat
      541 cgtcacactt gattgagaaa cgaagctcct tagcttcaac gggttgcttg agcggagcaa
      601 gaatactctt tatggcttcg cccgacttat ctcttgcgag cagaattaca attttagtca
      661 taagatatgc gccgtcgtca aggtaataat tctccttgaa cgccgcatga cccgaggtct
      721 ccatagcaag ggggcagaac ttacctgact tgttcaattc aatctgcttg tttataacgt
      781 ttctgtagcc tctcttgaag cgataatgct cgcctccgat cgactctatg tactctttca
      841 gtccgtccga ggttacggaa tcggtaacga taacgccgcc gtcgtgaccc tcaagagcta
      901 tgtaagaagc aagcgcaaca agacggttac ggttaatttc ctcgccgtta ttctcaacgc
      961 agcccgctct gtcaacatcg gtatcaaaaa ttattccgag gtctgcatgg ctctcaaccg
     1021 ttgccttcga gacagagtcc atagcttcgg cattttcggg attgggtata tgattcggga
     1081 acattccgtc ggggtcaagg aactgactgc cctcaatatc tgcaccgaga ggagcgagaa
     1141 catcggtcgc atagaagccg ccgacgccgt tgcctgcgtc aacgataatc ttgaagcctt
     1201 caagcggctt gttatagttc tcggcattaa cgcccttctt tatcatctct cgcagatgca
     1261 agcaataggt tttcatgtag tcattctcaa cgcaaagcgt cggttctgct ctgtatttct
     1321 tgccgtagat ttcctccgcc gttttgagaa gctcggcgat gttttcaccc tcaagtccgc
     1381 cgtccggggt gaaaaattta aggccgtttc tctcccacgg gtgatggctt gccgttatct
     1441 gaattgcgcc gtcgcattta aggtcaagcg ttgacatgaa catggcagga gttgaggcaa
     1501 gaccgcagct atagacctta acgccctttg cagtcagcgc agtgatcgcc gccgccatta
     1561 ttctcggtcc ggaaacacgg gaatcacgtc cgattgaaac tgtaatctca tttgtgtatt
     1621 tccccgttct tgcgccgagc cagtttgcga agctttcggc aacagccatt acaaattcat
     1681 cgctgagata gagcgtctca tcgctgaact gctctgctcc gtagccgcgg atatccgtgc
     1741 cgctcttgaa gcccttgaaa ttcattttaa catcgtcctt tcatctgata taccctttaa
     1801 gaaaagataa agctcggcgt attcgtcgaa agccttgccg acttcatcaa taactttttc
     1861 gctttgcaga gttttgaaat ccgttctgac cgccatatag cctattgatt tcatattgta
     1921 atagggttca acctccggaa caggctcacc cggctttttc tttttatagt agtcattcaa
     1981 acgggcagta cccacctttt ccgcacggcg aacagcatga agaaaatcct cgggatgctc
     2041 gataagagcc tttctgtaaa gctcccagaa ctgagcgtcg gcatagtatg tgccgacacc
     2101 gtagccccag caatcctgat tgacctcgaa ccacatacag ggataattca cccattcacg
     2161 cttgtttcgc atgaacatca cccaaaggtg gtcacggtac agtgatttat ccttggtgta
     2221 gcggttgtca cgtctgattc tcgacaccat tctgacgggg tcggtaacta tcatgtcgtc
     2281 aagctcaaag accttgcccc cgagagccat tgcaatttga cgaagcggaa cggtgaagcc
     2341 gtccttcagc tcctgcttgt gctcctcgta aaatgccttg ctgttttcaa atctgttctg
     2401 cgccatgagc gcgagtgttt cgggaaaaat tccgtcgtat ttcaattata tcaatcctct
     2461 ttttgccatt tcctcggcgg caagcattat gccgaccttg gcactgtgga aattaagacc
     2521 gccctgcata tatacggcat agggctctct gagcggagcg tctgcggaaa gctcgattga
     2581 tgcgccgccc gtgaatgctc ctgccgacat gataaccttg ctgtcgtagc cgggcatatc
     2641 ccacggctcg ggcgttacga atgagtcaat cggcgcaacc ttctgcataa cctgacagaa
     2701 ggctatcagg ttttcctcat tgcgaagaag aacgctttga acgatatcaa atctgtcgtc
     2761 cgtgcttttc ggagttgtgc cgaagccaaa ctcctcaaaa agtgcagcgg caaagcgtgc
     2821 ggatttaatt gcttcgccta cagtgtgcgg agcgaagaaa agtcccataa aaagctcacg
     2881 ggaatggccg agcgttgcac cgacctcacg tccgagaccc ggagtcgtca gcctgtagga
     2941 aatcttttcg acaagatctg ctctgcctgc gacatatccg cctgtttttg caattccgcc
     3001 gcctgcgttc ttgatgagcg agcctgcgat tatatccgct ccgacctgag tcggctcgat
     3061 tttctgagtg aactcgccgt agcagttgtc aaccataacg tcggcactgc tgtttgcctt
     3121 gacaatttca attatcttcg ctatttcctc gcaggaaagg ctcgggcgca gggaatagcc
     3181 tcttgaacgc tgaatataaa caagcttcgg ctgctcctcc tttaccctct ttgctatctc
     3241 atcgtaatcg ggcttttcgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgagcc
     3301 atcgcccttg ccgtcaattc cgatgacgga atgaatcgtg tcatagggaa gtccggtaac
     3361 gcaaagcatg gtgtcaccgg gtctgagcat accgaaaagc gcaaccgcca aggtatgagt
     3421 gccgcaggtg aaattgtgac gaacgagcgc atcctcggcg cagaaaatat ccgccgcgac
     3481 cctgtcaatc acgtcgcgtc ccctgtcacc gtagccgtag cccgtgctct cggcaaacat
     3541 cgcctcgctg actccgttct taataaaagc ggaaagaacc ttttgttgat tgtattcggt
     3601 aatttcatct gtctttgcga aacagggctt aatcttctcc agcgctttat ccgccgcaga
     3661 agctaccttt tcgctcacct gaaaaaattc attgttcatc ggttattata ctccattcgt
     3721 tttttacttc aaatccaatt gatttataaa attcaactat actgtcgtgc ttgcaaagaa
     3781 gctcgacacg cttatcgttt tcactttcgg caagccttgt cacaagtgcg cctgccagcc
     3841 ctctgcctct gtattcggga cgggtcgcaa ccgctccgag aagcaccgcc gtctctgtcg
     3901 agaaaagctt catcgcacat gcgacggttt tcccgtcctc aacagccgat aaggccgagg
     3961 tcactcctct gtttattcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnt ttgccaattt gattatatca taaatttcct tcggacggaa tatgttatcc
     4141 gcctgaattt ctttaatttc atttttattt ttaatgtatt ccacaacgta accttcaaga
     4201 ctcgatttcg cgcccatcag ctctgcgatt cttttctcgc actgaacggt gcaaaagccg
     4261 acaatttgca aaaaagcatg gatttcttcg gcattttcgc ccgttgatgt gagcgttgcg
     4321 tctccgtcaa ttctgctgac tgcggaaatt atattcccgt catcgtccgt ctgtttccag
     4381 aatttcacga aatcgtaatc cgtcgaatag caattataaa ggcatgatat ccttgtgccg
     4441 aaaacgtcac gccgacaaaa ttcgacaaaa tccttgtcgc ccttttcgac cgccttaagc
     4501 atcggcaaac tcctcggcaa gcgcacggat aatgcgttcg ctctcggtta tatcctccgc
     4561 cttcagcacg catgacggcg aatgaagata gcggcacggc attgaaacag tgacggtttt
     4621 tatgccgcct gccgtcttgt ggataacgcc tgcattgttg ccgcccgcaa ccgccgactt
     4681 gacctgcgcc ttgattccgt cacgcttcgc aagctcaaat gccttgtcga aaagctccct
     4741 tgaataaatc gtgcgtctgt ccatgaacga aataacagca ccctcgccga gcttgcagac
     4801 ctttttattc tcgggcacat cggcaagatc agccgcagtc gttgtttcaa cgactatcgc
     4861 ataatcggga tcaactctgt atgccgccgt ccctgccgag ccgagtccta cctcctcgcc
     4921 cgttgcgaaa ttaaaataca tgtcatacgg caggtcagat tttatcattt tgataagtat
     4981 tgcacagcct gcacggtcgt caagcg
//
LOCUS       DAWBSW010000152         4879 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3098_length_4879_cov_2.385365, whole genome shotgun sequence.
ACCESSION   DAWBSW010000152 DAWBSW010000000
VERSION     DAWBSW010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3098_length_4879_cov_2.385365"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1484..1493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1416 a         1060 c         1019 g         1374 t
ORIGIN      
        1 agcggaatga acgtaaagcc gttttcgttt gcatatgtct ccgccgccgt tcctctgtag
       61 ccgtagattg tgaagccgtc atatttctta tactcgtttt tttctgtagt ataataccca
      121 aacgcttttt ccccgataga agttacagaa gcaggtactg taacggattt aagcgaacgg
      181 caaccaagaa aagcattttc accaatagtc tccaagccat cgtttaattc taacttcacc
      241 aaacttgaat tatcatagaa gcttttttcg cctatacttt ttaaagtcga agggaatttc
      301 aattctttta tagaattact gttttcaaag cttctgttac caatgctttc caagccttca
      361 ttcaaattca ctctttcaag ataaaaagtt gagtgaaagg ctccgtcctc aatctcggta
      421 accgaagacg gaatagtaat actgagaaga ccgcaggtat caaaacagtc aacgggtatt
      481 tttttaatgt tttggtttaa aaaattaacg taaattaaag accaacactc ctgaaacatt
      541 gccggacaca aatcgcttcg aattgtcgcc cttctcaaaa agcttgctcc gaatacacac
      601 tctgcaccgt tttcaaaaac tacattggat gggatatcaa tatcggtaat gcctgtgtag
      661 caaaacgcaa aagaactgag aatgatttta cctgttccgt ttattacaag gcgctcaacc
      721 tgtctcgtaa cactgccgga aaaagcggat ctttttatta caatcggttc ttctcccaat
      781 gcgtttatag ttaccgtttt tacgcactta aattccggat catattctcc gttaaatctt
      841 tcctcagaat actgattcgc ctcaacaaca tcgccgctgt tgagtgttac gtcatatgtc
      901 ggataatcgg aagcatagac gttggttgtt atcattgcca aaatcacaat gataaccaat
      961 ataattctca ctcttttttt catattgaat cccatcctta actgtttatt tagcaataat
     1021 gaactaattc atcagtccac tactgttccg ttatgactct ttatattatc aggcacatta
     1081 aaagatgtat agcaatcata actacctgat ataaaaagat ttgcaccacc attatttatg
     1141 aagtagaaac cgtcatctgg cacatcattg ttgccaatag gttcttctat accgtaatag
     1201 gctttacaat tgtgaaaacg gtatgtagat tttttcccat catttccttg tacaaaatat
     1261 atacgttttt tactgaaaat ccttctgaaa ttactgaatt cacaattatc aaaaagaaag
     1321 tcccatgaga acatacaact tttattaagc gaaacgtcat atgtttttat tccattattc
     1381 tcctatatct ctttggtttt gttaaaattt acttctgtaa tatcaataaa cattgctttt
     1441 cttcctttta aaatatattt ttaaaagtcg ttagaaataa ccgnnnnnnn nnngattagc
     1501 tccgacaata aaaatcggaa tcgttgataa agtcaaaaaa cagacttcaa cagtcattgt
     1561 taacgactta ccatagcatc attttttcgg aaatgaacgt tcaaagttcc cggatcagct
     1621 tccctttttg ctaatccgta atttcattat atatgaacat ttgttctttg tcaatggttt
     1681 ttgttaattt tgttcacaat gttatcgatt ttgttcacga ttataatttt atttcggatt
     1741 ttttgtttaa tgtgtatagc gatttttgca tacttgtacc gacaaaaccg cattgtatat
     1801 atgattctcc ttttctccgc tttttcacgc ttttattaca actaattgtg aaaattaaca
     1861 gtttttgacc cgcaccggtt agtttattct aactgagaaa agttgcaaaa atgattatat
     1921 tatagtataa tttgtttgaa tggttaaaat accattatca ggatttagtc attgctttca
     1981 actttgttta aatccaagaa aacaaaaaga aagtcaggcg atttttatga aaactgttta
     2041 tgcagacaat gctgcaacaa cagctctctg ccctgaagct cttgacgcaa tggtagattg
     2101 tttaaaaaac aactacggta acgcttcaag tctgcaccac atgggtcagg aggctgccgc
     2161 cgcagttttg caggcaagac gtgacattgc cgacgctatc ggcgccgatg tgaacgagat
     2221 ttacttcacc tcgggcggaa ccgaagcgga caactgggca atcaccaatg ccgcacatct
     2281 cggcgagaaa aaaggaaaaa agcacattat ttcaacaacg tttgagcatc atgccgttct
     2341 tcacacgctg atgaagcttg aaaaagaagg ctttgaggta acctaccttg atgttcatga
     2401 ggacggctac gttcgtcttg acgagtttaa aaaagctctc agagacgaca cctgccttgt
     2461 tacaataatg tatgcaaaca acgaagtcgg aacggttcag cctattcccg aaatcggcgc
     2521 aatttgcaaa gaaaagggcg tttatttcca caccgacgct gttcaggctg tcggtcatct
     2581 tccgatcaac gttaaggaac agaacattga tatgctctcg ttctcatctc ataaattcca
     2641 tggccccaag ggaatcggtg cactttattg ccgcaagggt attcccctca tgggcttcat
     2701 gcagggcggt gctcaggaaa gaaacaagcg ccccggcaca actaacactc ccgccattgt
     2761 tgcaacggcg gcggcgctca aaaaatgctg cgaggacatg gacaaaaaca ttgcctatgt
     2821 cagtaaatta agggacaagc ttattgatac tatccttgaa atcgttcccc gttcaagggt
     2881 aaacggagga cgctcgcccc gtctgcccgg caacgttagc ttctgctttg agggcatcga
     2941 gggtgagtcg cttctgcttc accttgacct caaaggaatc tgcggttcct cgggttcggc
     3001 ttgtacctca ggctcactcg acccgagcca cgttctgctt gcactcggtt tgcctcacga
     3061 gattgctcac ggttcgctcc gtctttctct ctgcgaatac aacaccgagg aagaaattga
     3121 ttatatcatt gaggagcttc cgaaaatcgt ttcgatgctc cgtgacatgt cccccgtttg
     3181 ggagcgaata atgaaaggag aggactacta tgctgtacag tgaaaaggta atggaccact
     3241 tccagcatcc gagaaacctc ggcaaaatgg atgacgcgga cggcgtcggc gaggtcggca
     3301 atgcaaagtg cggagatata atgaaaatgt acctcaaaat taaggacggt aaaatcgagg
     3361 acgttaagtt caatacgttt ggctgcgctt cggcaattgc cacaagctcg attgcaaccg
     3421 agatgatcaa gggtcagaag attgaggatg cactcaagct cagcaacaag gctgttgttg
     3481 aagctcttga cgggcttccg ccggcaaaga ttcactgttc cgtgcttgcc gagcaggctg
     3541 ttaaggcggc tctcaaggat tattacacca agaacggtct tgagcttgat ccgaacgtat
     3601 tcggcagtga atgcggaagc aactgcgaaa gctgcgaggg ctgcagccga gagggctaag
     3661 ccatgaaaaa ggctatgatt gccatgagcg gcggagttga cagctccgtc gctgcttatc
     3721 tgataactca gcagggctat gaagcgatcg gcgttacgat gaagctttac aacaacgagg
     3781 atatcggtat aaaaaaagaa aagacctgct gctcactcga cgacgttgag gacgcaagga
     3841 gcgttgcaaa caggctcggc ataccttatt atgtttttaa ttataccgac aattttaaat
     3901 tgcaggttat cgaacgcttc gttgaagcat atcgcaacgg cagaacaccg aacccgtgca
     3961 tcgactgcaa cagatatatc aagtttgatc agctaatgca cagagcaaag gaattgcagt
     4021 ttgattatgt tgtaacgggt cactacgcca ttaacgagta cgatgaagaa aagggacgct
     4081 ggatactgcg caaggcgacc gacgacacaa aggatcaaag ctacgttttg tattcgctca
     4141 cgcaggatca gctcagcaga atcctttttc cgctcggcag aatgaccaag catgaggcga
     4201 gggaaatcgc cgaagcgcag ggctttgtca acgcagataa gcacgacagc caggatattt
     4261 gcttcgttcc cgacggcgac tatgcccact tcattgagaa ttggtgcgga ttcaggttca
     4321 ccgagggtga ttttgttgac cgtgacggca attttattgc caagcacaag ggcataatca
     4381 actacacgat cggtcagcgc aagggcttgg gcattgcggc gaaagcgccg gactatgttg
     4441 tcagcaagga tcttgcgaaa aatcaggtcg ttctcgggtc aaatgacgat ttgttctcta
     4501 atcaccttga agcaactgat ttgaacttca tttcgattga aaagctcacc gagccgatga
     4561 aggtcaaggc aaaggtcaga tacaggcaaa aggagaccga cgccgtgatt actccgctcg
     4621 aaaacggaaa cgtcagcgtc gattttgaaa cacctcagcg tgcgattgct cccggccagg
     4681 cagttgtttt ctatgacgga gacatagttg tcggcggcgg aacgataata taaattaaac
     4741 aattaataat ttatattata tatattataa atgtatagga ttcactctcc ctgtttttgt
     4801 acgggagagt gaatttttat cggctgattt acttgccttt ccctgcagag gaggagctta
     4861 cgctaaaaga taaattctg
//
LOCUS       DAWBSW010000153         4850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3117_length_4850_cov_2.424192, whole genome shotgun sequence.
ACCESSION   DAWBSW010000153 DAWBSW010000000
VERSION     DAWBSW010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_4850_cov_2.424192"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1290..1299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2105..2114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1601 a          860 c          986 g         1383 t
ORIGIN      
        1 aaaagacatt ctttatcaaa gtgttgactg ttgatgccga cgtactcgtt acaaaggcgc
       61 agtccgatga aatcaagagt gttgctaaaa aggtttatga agctgtaaga tattccgacc
      121 ctatgtcaaa cgataggctc gcaggtgttg aaatgagaat agaaaatctc tttgacgaat
      181 ttaaaaatgc tgtttctgaa aacgatgcag cttcttcaaa gagaattgcg gcagatttgc
      241 ttgaagcaat agatgaaaga aataaaaagt gtaagatgtt gaaataatca aggggtgcaa
      301 catggcagtt tttaagtgta aaatgtgcgg cggttccttg aatgttcagg atcattcaag
      361 aattgttgaa tgcgaatatt gcggaacaag gcaaactttg cctaaattgg aaaatgaaaa
      421 gacctccggc ctgcttgaca gagccggaga ccttcgccgt caaaatgaat ttgataaggc
      481 agaagctctt tatgagcaga tattggagat ggacaacacc gacgcagagg tttattggtc
      541 actggtgctt tgtaaatacg gcatagaata tgttgaagaa ccgggtacag gcaaaagaat
      601 cccgacaatt cacagagctc agttcacgtc aatatttgcc gatgaaaatt tcaaaaaggc
      661 attagaatat gccgacgatg agcagcgtca gctttataat gaagaggcca gcaaaattga
      721 gcaaattcaa aaggatatta ttgaaatatc ttcaaaggaa gagccgtttg atgtttttat
      781 ttgctataag gaaagtgatg aaaacggtaa tcgcactgag gatagcgtta tagcctatga
      841 tttgtatcag aatttaacaa agaacggata taaggtattc ttttcgagag taacacttga
      901 aagtaagctc ggaacagcat atgagccata tattttctct gctttaaatt ctgcaaaagt
      961 tatgatagta atcggtacaa agccggagta ttttaacgct gtatggatta agaatgagtg
     1021 gagcagattc ctcaagatga tgaaagagga taaatccaaa acgctgattc cggcatataa
     1081 aaatatgaat ccgtatgctc ttcctaaaga attttcatat cttcaggctc aggatatgtc
     1141 aaagctcgga ttcttgcagg acattactca cggaatagat aagatacttg aaagcaataa
     1201 agaaaccgaa cagactgaaa agagttctga aaagccgaca gataagatca aaaatctttc
     1261 taaaaaaaat aagattatta tttcgattan nnnnnnnnnc tctgttttgg ttgttgcaat
     1321 aattgtcgga gctgtcgtta taccgaaagt gaaaacgagc agagagaatt cgccaaagga
     1381 taatacagca agtgataatg ctcaagatca aacaacggca caatcatctg tttctatcct
     1441 tgatgagagc acccaaaacg caatagtaaa ggcctatgtg aaacaattag aagaagcggt
     1501 aaccttttat ggagtattat cggaatatac aatttacgat attgacaata atggtatacc
     1561 tgatttgatt tacagaggag gagattaccc gggaggagga cttggatatt tttgttttta
     1621 cacatataca gataaaattg aagaaatgaa gtctttgtca gcatctcatg caagcttggc
     1681 aataccttta tcaggaagtg gattgatcct tttgagagga catatgggat attgttatgt
     1741 gagcagaata acatatagca aagatgttgg atttacagag aaagaattaa ttaattatga
     1801 aatatctgct gaccaaaatt atgatgatta tttcagagca tatgccggca cgtatcttta
     1861 tactttagag caaattgatg agccagctat atcagagttg gtaaagttgg ggtacaacaa
     1921 atatttagat tcaattggag ttacgccaaa gaaagtaggt tcaaccgaat catatgaaga
     1981 gtcggagaca tatgttgaga tgaatgacta tactgatgcc gaagtgaaca actattccga
     2041 agaaccggtt gattatggct ataatgatac tcaatcagac gccggctaca gtgaagtaga
     2101 gatannnnnn nnnncagcaa taatttcacc cttgccggca gataacaact tgaaaccgct
     2161 aacggaggac ttgagccctc cgtttttcta tgaaatattc agaactttgt ccatagtttt
     2221 tgctgcggct tgactcatgc tgtcaaggga atgaccgtaa acgttaagaa ccattgttcc
     2281 gtcgctatgt cctaaccttg ttgcaatgga ttttatgtca acattgtttt taagaagcat
     2341 agttgcacaa gtgtggcgta ggccatgtaa tttaagtctg cgtattccgt tagcggcaag
     2401 aaatttattc caccgccttg taaaattatt tggattgtcc gtctgcccgt cttcacggat
     2461 tataactaaa tctgatagtg attgaatacg gccacaacga aacaacacct cttttgacca
     2521 tgtgcgataa cgaagaaatt cctgtcgagc ttgctgactt aaaaatattt ttcgtttact
     2581 gctttctgtt tttggggtat ctattatttc tttcccgcaa gcatcaagcc gtgtctgtac
     2641 aatgtcgatg taagggtgtt ctatatcgtt gtcaatatga acatctttcc acataagccc
     2701 aaaagttctc ctcttctcat accggtatga ataagtaaat atatggggaa ataaagatct
     2761 gttccttttg cacaaagcag aatctgacgt atttcgtcct cattaaatgc ctgtacttca
     2821 tatttaacca cgggaggaag atttgcatgt ttgcaaggat tatcgggaat cagcttcata
     2881 aacacagcat aattaaaagc ggcttgaaga ttaaggaaaa tatttttaac gcttttaggt
     2941 ttaagcggct tagcttcatt cttctttgtg ggtgggtgag agtatatatc attaacccag
     3001 ttttgaatca tattcacact gactgccgaa acaggatatt tgcctaataa aggattgata
     3061 tacttctaaa gcaatatctt ttatttgcgc tttataattt ccgtctttta cctgcgtaaa
     3121 tttctgagaa gtttcctcgt ttaaaaattt agtaaatatt gacatgttca atatctcctt
     3181 tataaaatta tttaaaaatc ataaattgat gcacctatat ccaaataatc aagtcggaat
     3241 ttttatgttt tgttgcagtc caatttatga gaatttttca agcatatttt aaaccatata
     3301 aaatgaaaaa atcgtgttac tgctacacat ttataaataa tttttatgaa cccaatgatc
     3361 gtatgcatgg agtaaaaaat gattataatt ctacaattca ttctatatga actattgatt
     3421 gcagacgtag tatagcacat aaaattaacc ccaaaacgtg ttacaaatac acattttggg
     3481 agataaaaaa gcctattgct ccaaagaaca ataggctttt tatagaggtt atatatggga
     3541 taaaatatgc acacggatta accatatgcc gaaaagaaag gcaaattggt aaactttgtt
     3601 tgcttgagcg gtcgtcgttt ggtcgtcatt tttacgggcg ggcgagctaa actcgttgaa
     3661 ttatatagtt tccattatag gtttccgagc tgctccaaaa ccacgtgccg aggggtacgg
     3721 gtctccggtg gagacctctg catacgaagt atgcagaagc accgaccgag ccggcaggcg
     3781 agaccaagtc cttctgcccc tgccagaacc aagtctccgg aagccttttt tgcaaggttt
     3841 ccggagactt tttagttaag gtaaaaatac ttctaagtta aagtattctt gggccaagat
     3901 ttccttagcg ccgcatagga aattacaaag ttggcaagcc agcctgccgg tactgcaagc
     3961 caaacaaagg caataccaaa acgcggtgcc aaaatcaatg caacggacaa acgaattgca
     4021 aggttaacca tatttgcaat taaaaacggg cgcatatttc caagtccgcg gagaacaccg
     4081 tctgttgcca ttttgatacc catgaatatg aaaaagtagc cgatccatct catataatct
     4141 ccggacactt gatatgatag agctgttccg tctttgccta agaaaagcga agaaatttga
     4201 gtgtgcatcg tttcaataat tacaaatgca agaacggcaa agcatgcatc taacagcaga
     4261 gcggcacggt aacccttctt aatacggcca atttttttag cgccgagatt ttgggaaaca
     4321 aatggtgaca cagcattccc gatggataca aatatcagtg aaaaaacatt ctctaccctc
     4381 atcgttgccg tatagcctgc gagcgcttgt gtgccgaaag gatttacaac tgcttgcaca
     4441 atcatcatac cgattgatac ggttgattgt tgaaggatag acggaacggc gattttaagc
     4501 attgtacgca gttctttttt gtcaaaccaa tgaaacaaac ctgcatattt tcgcatacgg
     4561 taaagaaaaa tcaaaagcga aagtacggct gaaatacctt gtgcaattag agtggcaaga
     4621 gctgcaccga atactccgag tttaagacct ctcaccatcc aaaggtccat aatgatattc
     4681 gaaacagacg aaaatattaa aagccataac gggatttttg attcaccgat tgaagtgaac
     4741 atggttgaca gaatattata cataaataaa aatggaaagc caacaaaata gatacgtaag
     4801 tacagcaccg catctttcat tatatccgca ggcgttttca gtatgctcat
//
LOCUS       DAWBSW010000154         4816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3136_length_4816_cov_2.819366, whole genome shotgun sequence.
ACCESSION   DAWBSW010000154 DAWBSW010000000
VERSION     DAWBSW010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3136_length_4816_cov_2.819366"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1431 a         1081 c         1045 g         1259 t
ORIGIN      
        1 gaataaaccg aacgacaaat ccgtttatca tatatgcagc atgaccgaat atatttttaa
       61 gcttctgcgg cgtactgtac gaaagatcag ttgcaattcc gaacgaagca atatatgtaa
      121 aatacctgcc gttcaatgta ccgaggtcat agctgttttt ctttccgctc tgtatcagat
      181 cggcggaagc accaagttcg gtcggaattc caagcgtgtt tgccaaatca ttggttgaac
      241 ccgacggaat gtagccgaca ggaatttttt tgtcaagctt cataagtccg ttaatgactt
      301 cgttaagtgt tccgtcgcct ccgcagcaaa gaataacatc gtattttgca ccctcgtcct
      361 taacaatggt ggttgcgtcg ccctggcagc gtgttgttct tatctccgtc tcccattcgg
      421 gcggtgaaaa cttcttgaca atctccaaag caccgaggcg cttgttgttt cgccctgcgc
      481 acggattgac tatcaaaagt gctttcttac tgtccataaa tgcaacccac cctttcttcg
      541 aacattataa ttataccaaa tacgtcccga tttcgtcaat gccttttgcg acgaaaaagc
      601 gaaattgacc taaattaaaa ttttattctt acaataaaaa taataagtaa aaatttatgt
      661 taattctgct taattttatt gacataattg tcgaattttg gtaatatata taataaaaat
      721 ttaaggagat gattttatgg atttgaggaa aattattgaa aaaaagcgtg attcggcaaa
      781 atatatgatt gaggaaatta catatatctg taaaaacatc gaaaagcgca gcccgggctc
      841 tacaggtgag aaaaaagcat gtgaatacat ggcagaggtg ctcaaaaagg actgcggctg
      901 cgaaagagcc gatgttgagt cttttaagga aaatcccggc tcattttacg gctggttgtt
      961 ttttactctc acctttgtgc ttgcggcaat agttctctat ttctttgcgc ctgttgcctc
     1021 gataattctc attgcagcag gtcttgtatt ggtgttgctc cagttcggac tttatatcaa
     1081 agccattgac aggctttttc ccgaaaaaac cggacacaat gtaaccgcaa tcaaaaaatg
     1141 caaaggcgaa accaaaagaa gaatcatctt caacggtcac ccggatgcgg catgggaatg
     1201 gcccgttaac tatgctctgg gcggtgtagg ctttgaggca catgctgtta taggctttgc
     1261 tggcgcattt tattatctta ttcttgcaat cattcagctc gtacagaacg gttctgccgg
     1321 cgcagatata tcgactccgc tcggcaaggc aggcctcatc ggtctcatat ttgttccgtt
     1381 ccttgtaggt ctttatttca tggtcaacta cagacgtgtt gtagacggtg caaacgataa
     1441 tctcagcggc tgctatatgg gaatcgctct tctcaaggct ctcaaggatg aaggcattga
     1501 gcttgaaaat acggaaatcg gcgttgttct caccggctcc gaggaagccg gtctcagagg
     1561 tgcaaaggca tggtgtgagg ctcacaaggg tgaatttgac gatgttccga cattcatctt
     1621 ctcttttgat acaattcatg acccgaaata tctcatggtt aactaccgtg acctcaacgg
     1681 cacggtcaag gcagatgccg atgcttccga cctctttatg gaagcggcag acgagctggg
     1741 aataccgtgc aaaaaaggca tggttcctcc tctcggcggt gcaacagaca gcgctgcctt
     1801 tgctcagggc ggtttcagag ccgcaggtat caccggactt aaccacaagc tcgaatcata
     1861 ctatcacaca agacgtgaca gctatgacaa catgaacctt gacgggcttt cagattgctt
     1921 tgccgcaacg gtaaaggtac ttgagaaatt cgacgagggt gcaaagcagt aaaaagaata
     1981 ttaaaaggtc tgctgccgtt tcccggcggc agatttttta tgtccgatgc ggttttactg
     2041 ttgttttttc gctttgattg ttatataata cttacatata caatctggag tgatagcttg
     2101 aataagaaga aaaagaaaat ccttataccc ctgcttgtta tatcgcttct cgttccgaca
     2161 tggtatgtca ataactactc tctgaagctg acagaacaga caatatattc tgcgaaggtc
     2221 aaaagagata taaaaattgc cgtagtctca gatctgcacg gcgaacattt cggtaaagat
     2281 aacaacaaga taacaaatct tatcagcggc caaaagcccg acctgatatt tgttctcggc
     2341 gatatgtata ctcaaaatcg ctatgagcaa atagataccg ccgttgagct tgtcaggaat
     2401 ctttccgaga ttgccgaaac ctatgttgtc acaggggacc atgatacgga cctgaaatac
     2461 aaggacgaat tgaattcact caaaaaagtt catctgatga actataaaaa agcagatcta
     2521 agcataaaag acacggatat caccgtctac ggcattgata atgtgtattt ctcgccaacg
     2581 tttgatttac acaacgaatt tgacaaaccg gattcaaaaa ggctgaatat actgctcgct
     2641 catattcccg aaacggagca ttttgaggat tttgaagcag atatcatatt cagcggcgac
     2701 acccacggcg gaatgattcg cttgcctctc atagggccgc tctactataa cggatatatt
     2761 ctgccgaagc tgacatattc cgaaaaaata acagataagg gtctttattc ccttgagaac
     2821 aagcatattt ttgtaacctc gggcataggc aactatcccg ttccgctgag atttttcaac
     2881 cgtcccgagg tatgtatgat aaccttaaaa aaccgttcat aacagaagaa aagcatcaat
     2941 acgttaaaaa ggagactgca aaatgagcta cgccgacaaa cttttcattg acatgtgtaa
     3001 caacattctt gaaaacggaa ccgacaccaa gggtgaaaag gttcgtcccc actgggcgga
     3061 cggcacaccc gcctacacgc agaagctttt ctgcgttgta aaccgatacg acctcaaaaa
     3121 agaatttccc gctctcacac ttcgccgaac agcgctaaaa tcatgcgttg acgaaattct
     3181 gtggatatgg cagaaaaagt caaacaatat tcatgatctt cactcccata tctgggattc
     3241 atgggctgat gagaacggct ctatcggcaa ggcatacggc tatcagctcg gcgtcaagca
     3301 taaatataag gagggcatgt ttgaccaggt tgaccgagtt atttatgacc ttaaaaacaa
     3361 tcccttcagc cgtagaatca tgaccaacat ctataatcac cacgacctca gtgagatggg
     3421 gctttatccc tgcgcatact caatgacatt caacgtcacg gagggcgaaa acggaaagct
     3481 tgttctcaac ggaattctca atcagcgcag tcaagacgtt ttgaccgcca acaactggaa
     3541 tgtcgttcag tacagcgtgc tgatgcatat gctcgcacag gtgtgtgata tggaagtcgg
     3601 tgaatttgtt catgttatag ccgatgctca catctatgac agacatattc cgctcgtcaa
     3661 gaaactcata acccgtgagc cgctccccgc tccgacattt tggctcaacc cggaaatcaa
     3721 ggatttctac gaatttacga ccgacgatgt tcgccttgac aactacgaaa cacatgagca
     3781 gataaagaac atccccgttg cgatatgagg tggcaaaatg aagctaattg cggcagttga
     3841 caatgaatgg aatatcggaa acaaaggagg cttgcttttc tccctgcccg acgatatgaa
     3901 gttttttcgc acaaccacat cgggaaagat tgttgtcatg ggcagaaaaa cgcttgaatc
     3961 cttccccggc tcaaaaccgc tgaagaacag ggtaaatatt gtactgtcaa ggtgcaatcg
     4021 ccatgttgac ggtgcggaat ttgcaacaag cgttgatgaa ttgcttgata agctcaaggc
     4081 atacaacttt gacgatatat atgttatcgg aggagcacag atttattcac tgcttcttcc
     4141 ctattgcgat acggcgctga taacccatgt tgacgccgtt gcaagcgaag ccgattccaa
     4201 gtttccagag ctaaaaaccg acgaatggaa aatcacggag caatcggata ttcatgaaaa
     4261 caatggactc aagttccgct ttacgaccta caagaggata taataatgtt cagcggcaaa
     4321 atgaaatccg tcacattcag ctatgacgac ggtgttacgc aggatattcg actggttgaa
     4381 atcctcaacc gatataattt aaaagcaacg tttaatctca attccgagct gctcggcaag
     4441 gacggaagcc ttgatattct cgacaaaagg atattgcaca acaaaattca gccccatgaa
     4501 atcaccgagg tttacaaggg ccatgaaatt gcggtgcaca ccctcaccca ccccgatatg
     4561 acggagatgc cggcagatga ggtcatacgt caggttgagc aggatagact aaatctcagc
     4621 gagctttcgg gcaccgaggt catcggaatg gcatatcccg gcaggcagcc gaattataat
     4681 tcaagaattg ctcgaattat caagaacggc acgggcgtta aatacgcacg aacaacgata
     4741 tgcaacaaag atttttcgta tcaaaacgat ctttacgaat tccatccatc tgtttttcac
     4801 cgagattggg atcagc
//
LOCUS       DAWBSW010000155         4778 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3159_length_4778_cov_2.621427, whole genome shotgun sequence.
ACCESSION   DAWBSW010000155 DAWBSW010000000
VERSION     DAWBSW010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3159_length_4778_cov_2.621427"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1164..1173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1391 a         1139 c         1022 g         1216 t
ORIGIN      
        1 gtgtcaatac cgaggttgac gcttccatcg aaagcatcaa tgccatgtat tcatcatgga
       61 tgataaccaa ggaaaagctt gccgaaaggt cggaatttct gctcgaacag cttcacgaaa
      121 aagagcttga gaaaaaatac cgcgaggatt ttattgccgg cacatcccat gagttgaaaa
      181 caccgatagc tattatccag ggctatgcag agggagcaaa atatgcgctt gacgataagg
      241 aaacgatcaa cgaatactgc gataccataa tcgacgaatg tacaagaatg aacgaccttg
      301 ttgttagcat gatgagtctt tcgaatatcc agcaaaactc ggattctctc aactatgaga
      361 acttctcaat caccgatttt atagcggacg accttgcttc ccattcaatt gtgttcaaaa
      421 agcacgggat taaagttgaa aacctcattc acaagcaaat aataggaaag gcggatttga
      481 agaagcttcc gtttgtttta aacaatctta tttcaaatgc aatttcctat attggaggca
      541 acccgaaagt tatccgcgtc cgctttgagg atgtcggact ttcctacaga atttttgttt
      601 tcaattcggg cgacaatatc cccgatgatg ttcttgaaaa gctttggttc agcttctacc
      661 gccacgatgc ggcaagaatg agaaacgagg gacatttcgg gctcggactt tcaatagtaa
      721 aaggaattca ggacgctcac tccgagcagt gcggtgtcaa caatgctgaa ggcggcgtcg
      781 aattctggtt tgatatcgga aaaggctcgt ttgacagcga cgaagaataa caaagcaaca
      841 gactgtatcg gcattgatct gctgatacag cctgttatat tttagtctat ctttttctga
      901 ataattactt cgcaaaatgc tcgacgattc tctttgccgc ttcttttgta tcgtcgggat
      961 caccgaaagc ggaaagtctg aggaagccct caccgcatga gccgaagcca acgccgggtg
     1021 ttccgacaat ttgaagctcg ttaagaagat aatcaaaaaa ttcccagcta cccatattgt
     1081 tcggacactt catccaaaca taaggggaat tcttaccgcc gacgtaccaa aatccgcact
     1141 tgtcaagagc atccatgata accnnnnnnn nnnatgataa cctttgcgtt ctttttgtag
     1201 accgaaatac tctcgtagat ctgcttctgt ccttctggag taaacacagc cgaagcaccc
     1261 ttttgcagaa tataagaaat accgtttgtt cttgtctcac ggtttctcga ccaaagtgca
     1321 ttgaggttca cgcccatacg ctcaagcttt gacggaacga tcgtatagcc taccctcgtg
     1381 cccgtgaagc ctgccgtttt tgagaatgaa caaacctcga cggcacaggt atccgcaccc
     1441 tcaatctcgt aaatactatg gggaacgtca tcctcctcga taaatatttc ataagcagcg
     1501 tcgtagagaa ttacggcgtc ttttttattt gcataatcga cccatttttt gagcttttca
     1561 cgtgtataag ccgaacccgt cgggttgttc ggggagcaga tgtaaattat atctgcgtcg
     1621 atactctcgt cgggaagcgg taaaaatccg tccccctcgc ctgccggtaa atgaatgacc
     1681 tttctgccgt agagcaggtt tgtgtcaacg tattcggggt aggcaggttc aattacaaga
     1741 accgtgttgt cacggtcaaa catctcaagc aaatcgccta aatcatcgca tgcgccgctt
     1801 gaaacaaaaa cctcgtcggt cgtcatgtca acattccaac ccgaataata cttcgcaatc
     1861 gcttcacgga gaaacattgc gccgtgctcg ggcatatagc cgtggaaggt ttctatcctt
     1921 gactggtcgt caacagcctc atgcaaggct tctatgacct ttttgcaaag cggatatgaa
     1981 acatcgccga caccgagctt caaaacgtgt tcgcccgggt gagcggcaag atattcgtca
     2041 gtctttctgt aaatagtgtt gaaaagatat gtgtccttaa gctccgtata atgcatattc
     2101 ggcttcatta tatatttacc tcgccttcaa aagatattct tgccgggcct gtcatcgtga
     2161 ctgtattatc ctcggcaata tttattatga gcgttccgcc gtcaagctca acggcaacat
     2221 cggcattgtt acgacaaaga ccgattgaaa tagccgccat aaccgttgca cacgagcctg
     2281 tgccgcacgc cattgttatt ccgcttcctc tctcccaaac tctcattctc agcttatttt
     2341 cggaaattgc ctgaacaaat tcaacattta cgccgtcagg gaacctgtcg ctgtgctcca
     2401 tagcggggcc gacagttgtt atttccgcct gaccgatatc gtcaacaaat actacggcgt
     2461 gaggattacc gaccgaaacc ggtatatatt caacgtcctt accgcagaca tcaattctgt
     2521 ttacaagacc cgcatctgct ttacccatgt tgaccgaaac ggaatcgacc ttgccctttt
     2581 cgatttttaa atcaagggtt ttgattcccg aaagagtctc aacggttatt tttgtttttt
     2641 tagtcaagcc cttatcgtag acatattttc cgacgcatct gattccgttg ccgcacatca
     2701 tcgcttcact gccgtcggca ttgaaaattc tcatcttaaa atcggcaata ttcgacggta
     2761 aaatccaaat cataccgtct gagccgatgc cgaaatggcg atcggaaagc ttaatagaaa
     2821 gatcctccgg attttctggt tctctttttg aataaacata aacatagtcg ttgccgagtc
     2881 cctgcatttt tgtaaatttc attgctgacg ccccctgaaa acaaaaaagt ctttaagggc
     2941 gcaaaatacc cttaaagacg tcattgcctt ttattttata atatgagtcg taaacctttt
     3001 tcgcaaatat tttaaccttg ctttaccgat ttgtcaagta ctttattcgt cttacataac
     3061 agacgaaata aaggaaaaca tattgacgga taattccaat tctgttataa tttttatgta
     3121 caaaacaaac agtacaaaag ctaaatacgg aggtacatta tgaaaactcg tgtcgcagtt
     3181 atggcaatca tacttgaatc aaaagaaaac gccgagcagc tcaataccgt tcttcacgat
     3241 tatcgtgaat atatccttgg cagaatgggg ttgccataca caaagcatga tatcaacatc
     3301 atcagcgttg cgcttgacgc tccgcaggat ataatcaact ccctcgcagg caaaatcggc
     3361 agaatcgaag gagtaaatgt aaaaacagcc tattcaaaca acgaatacaa cgtttaagag
     3421 ctttcaaaaa ggggccgtcg ctaaatgcga cagcccctca ttttttgttc actgttactc
     3481 tgtataagca acggaattgt catagagctt ttcgatatcc tcactcggct tctttgtgat
     3541 aagcgttacg acaacagccg caatcatgct tgcaacaaaa ccgggaatga tttcataaat
     3601 accgatatcc gaaaggaaca caagccagca gatatcaaca actgcaccga caacgattcc
     3661 ggcaacagca ccgccgaaat tgaagcgctt ccagaaaagg ctgagcataa tagccggtcc
     3721 gaatgccgca ccgaaaacgc cccatgcgtt tgaaacgagc gacataattg agccggagct
     3781 cgggtttgat gcaataagga gagcgcacac ggcgattaca agaacaacaa ttctgccgga
     3841 ccaaagcatc tctttgtcgc ttgccttgtt cttcctgata acgggctgat aaacgtcggc
     3901 agagaatgag gacgcggcag caagaagctg actgtccgcc gtgctcatcg aagcggcaag
     3961 aaccgctgaa agaagaatac ccgaaataac gcccgggaaa atcttacgaa ccattgtaat
     4021 aaatacaagt gagccttcat tgatctcctc gttaaagcca aatgcaagac gtccgatgat
     4081 gccgataagc gcagcaaaga taacgatgag aacagtccat gaaataccga tcttcgctga
     4141 ttttttaagg tctttctgag acttaagcga cataaatctg attatgatat gcggcatgcc
     4201 gaagtagccg aggccccagc cgagacccga agcaatgtcc ttccagtttg caaaagcgtt
     4261 ccagaaaccg ggagtctgct gagtagcaac agctgtgtcc gaaagagtga gaaccgatgc
     4321 ggcggcaatc ggagcgataa gcatagccgc aaggatgagc ataccctgga agaaatctgt
     4381 ccagcaaacg gcattaaagc cgccgaggaa cgtatagccg acgatgataa cggtcgctat
     4441 atacatagcc gtgctttctt taatcccaat aaccgtgtga aaaagagttc cgcaagcctt
     4501 tacgcttgat gcggcataaa ccgtgtaagc gacgaggaaa acaacagcgc agatgacctg
     4561 caaagccttt gactttgaaa ggaatctgtt tgtgagatac tgcggaatcg tgattgaatc
     4621 gttagcaacg atagagaagc ggcgcagtct cggagcttcc acaatccagc ttatcgtgta
     4681 acctatcgca agtccgacgg gaatccaaac ctgaccgaga ccgagagcat agattgaggt
     4741 cgggagaccc atgagaaccc atgcgctcat gtcggatg
//
LOCUS       DAWBSW010000156         4771 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3162_length_4771_cov_2.611747, whole genome shotgun sequence.
ACCESSION   DAWBSW010000156 DAWBSW010000000
VERSION     DAWBSW010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3162_length_4771_cov_2.611747"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    334..343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3267..3287
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1153 a         1278 c         1053 g         1256 t
ORIGIN      
        1 cttgttgaga acgcccgact ccttcatttc attgtaaagg tcgttaccct cacgggttct
       61 ctcaccgacg cccgtaaaaa cggaaatacc gccgtgctgc tttgcaatat tattgataag
      121 ctccatgatg agaaccgtct ttccgacgcc tgcgccgccg aaaagtccga tttttccgcc
      181 cttggcatac gggcagatga ggtctataac cttgattccg gtctcgagaa tctcggtcga
      241 ggttgaaagc tcatcaaagc tcggagcggg acggtgaatg ttccacctct cctcagtttt
      301 cggggtaggt tcgttgtcga ctgcctcacc gagnnnnnnn nnncgtccga gcgtctcttt
      361 gccgacaggc acacttattg catgacctgt atcggtaacg ggcgcacctc ttgttaagcc
      421 gtctgttgac gccatagcaa tacagcgaac ggtattgtct cctatatgct gggcaacctc
      481 aaccgtcagc gttttttcgc cgataggaac tgtcagcgca ttgtttatct caggcaaatt
      541 gccctcatca aagcgcacgt caacaacagg acccataacc tgagctacgc ttcctatatt
      601 tgaattttcc aaaaatatca ctccttaaaa ggattatgct ccgctgcctg cgatgatctc
      661 cgtaatctcc tgagtgatag caccctgcct tgcgcggtta tactgcaatc tgagatcgtc
      721 aatcatctgc tgtgcgttct tgcctgcgga atccatcgcc gttcttctcg ccacaacctc
      781 gcaggtgtag ctctcgcgca cggcaccgaa gataattccc gtaacatact cgggaacagc
      841 cgtatcaaga atgtattcct cgttcggttc aaaaaccgta ccggcaccgg attgagattc
      901 ttccttttca agcggaagca cccattgagc ggtcgcctcc tgcgtcatca tcgaaacata
      961 atgtgtgctt attataccaa gctttccgaa ttcgccgttt ttgaactcgg tgcagagctt
     1021 ttttgcaagg ttataagcat cagccgacgg atatttttcc gaggaattga cctctgcgct
     1081 atacctttca caagctcgct ttcctatagg gataaactcg gcatccggat actttgccgc
     1141 ttcgcggaac acattggcgt tatagccgcc tgcaagtccc ctgtcgcccg caattacgat
     1201 aagcttcacc ttatcgcttg cgttgcctgc gatgaacgga ctttttttgc actctgcgca
     1261 agcggaaagc tgactgacta ttactccgag agccgaggaa tagtctcggc ttttgagcat
     1321 tgccgccgtc gctcctctta tttttgagga agcgacaagt cccatcgcct tggttaaatg
     1381 cagtgtggaa tcaacgcttt tgatacgaat tctgagagat tttatatcag ctcccattca
     1441 tatcacctct gcatttcctt gagcacagtt tcgatttcgt ctatgtcaca ctgttcaaag
     1501 gagcctgctt caaggcggtt gagaagctcc tgcctctccg cctcaagctt tgaataaagc
     1561 tcctcctcgt aagtcttgac cttactgacc tcaacgctgt caaggaagcc gtgaataacg
     1621 gcataaataa taactatctg acagcctgct ctgagcggat gatttctgtc ctgcttgaga
     1681 acctcaacaa ttctttcgcc gagggcaagt cttttctttg tatccgcatc aaggtcactg
     1741 ccgaactgag caaacgattt aagctcaaga tactgagaat agagaagctt caattcaccc
     1801 gaaacttttt tcatgccctt gagctgagcc gaaccgccga ctcggctgac cgaaataccg
     1861 gggttgatag ccggcataat acccgaatgg aaaagctcag tttcaaggaa tatctgaccg
     1921 tccgtgattg aaataacgtt ggtcggaata taagcggaaa cgtcgcctgc ctgcgtttct
     1981 ataagcggca atgcggtgat cgagcctccg ccgtattcat cggcaacgca cgccgaacgt
     2041 tcaagaagcc ttgaatggag atagaaaacg tctcccgggt aagcctcacg tcccggcgga
     2101 cgtcgaatga gcagggaaag tgcacggtaa gctaccgcat gcttgctcaa atcgtcatat
     2161 acaatgagga catctctgcc cttttctctg aaatactcag ccatagcgca gcccgaatac
     2221 ggtgcaatgt actgcacggg cgcactctcg gaagccgagg cggaaactat aatcgtatat
     2281 tccattgctc ccgctctttg aagattctga gcgagctgaa caacggaagt gttcttctga
     2341 ccgatagcaa catagataca gataacgtcc ttgccctttt ggttgattat cgtgtcaacg
     2401 gcgatttcgg acttacccgt ctgacggtcg ccgatgataa gctcacgctg acctctgccg
     2461 atggggatca tgctgtcaat agccttgata cccgtctgaa gcggaacaca aacgctcttg
     2521 cgctgaataa ttccgggggc ttcggactcg atcggatgag ttgccttgca attaacgggt
     2581 cccttgccgt cgatcggctc gccgagtgca ttaacgactc tgccgagaag ctcctcgccg
     2641 acgggaacag agagaacccg tcccgttctg ctgacttttg agccttcctt tatcttgtca
     2701 gtctcggaca taattgcaac ggaaacggtc tcctcctcga gattctgcgc aaggccgaag
     2761 cttccgtcct caaactcaag aagctcactt gccatgcagt tacgcaagcc gtataccttt
     2821 gcaataccgt cgccgacaag gataaccgtg cccgtctccg ccatttcgat ttttgattta
     2881 tagtttttga tttgttcttt tataatgttg ctgatttcct caggtctggc gttcacctat
     2941 acatcgctcc cttcaattca tgcaacttct gtttaacact gccgtcgata accttgccgt
     3001 caaccgtgac cttcacgcct ccgataagtg atttatcgac actgcaatca agctcgactc
     3061 tatgaccgca aagatcttca agctttgatt tgagggcagt tttttcatcc tctgtcaatt
     3121 caactgcgct gataacctcc gccgtcgaaa ctccgtcagc ggcgtcgctc agctttttat
     3181 aatccgatat acaatccttc agcgtgtgaa tcctgcccct gtcgcaaaga aggcacagga
     3241 aagagaccac cgtttcggga atttttnnnn nnnnnnnnnn nnnnnnnacc gacgcgctcc
     3301 tgcttcggaa tatccggaga ggaaagaaaa tcaatgtatt cggggttttc tttcattaag
     3361 ttcagaacaa tatcgagagc ctcggaatat tccttggcgc tccccgtctc caatgccagc
     3421 gcaaaaagcg cctcggcata ttcgttactt atctgagcca tcgccgtcac ctatcccctc
     3481 tatgaaagag tcgataaatt tccgctgatc atccgaatca atctcacgct cgagcatttt
     3541 ctcggcaacg agcgttgaaa catcggcgat ctgatgcttg atatcgtccg aagctttttt
     3601 cttttccagc tcaatctcac tctgagcctg acggagcata ccgtcggcct tttccttagc
     3661 gtccgtgatg attttttcgc ttcttctgtc tgctcttacg gtggcgtcct tgatcatctc
     3721 gtcagtttcc gccttgaccg tttgaagctt gtcctcgtat gttttcttat cggcaagggc
     3781 ggcattttca gcctcctgcg ctctttcgta ctgtgcgtca acattagcct tgcgcttgtc
     3841 aacgaactta cgcacacggg cataaagaaa cttcttgaac aaaaggaaaa ttatcagaag
     3901 attgcaaagc gagatcagta tctgccatat attgaccgat atgacatcaa gattttgcat
     3961 tacaaattca ctcccgacaa aatcagatta agttcataag aatatcaaca agtctgctcg
     4021 gagcaacgaa gatgagaaga attgcgataa caagggcgta aagtcccgtt gtctcggcaa
     4081 tggcacaacc catgatcatg gtcgttgtaa cgttactctt gcattcgggc tgacggccga
     4141 tagcttcaca agccttggca actgcgttac cttcaccgat accggggccg atacctgcga
     4201 tcattgccat acctgcgccg agagcgcagc atccgagaat aattccgatt gcgattgctg
     4261 tcataataat aacctccgtt tatttatgcg acttttttcc ttgccgcttt tttctgttgt
     4321 cttttttcct ttcgcttctg ccactcctca atcgggaatg cacccgaaac atagatcata
     4381 gtgagcattg cgaaaatgaa agcctgcata catccgctga aaatgtcgaa atacagcgac
     4441 aaaatagcgg gaagtccgat ctggaacagc ggaaattcgc cgattttacc gggaatccag
     4501 ctcaacaaag cgtgcgacac agcctgcaag cctgccgcaa cgagagcgga aatgaccgaa
     4561 cccgaaagca cgttgccgta atgacggaac gacattgaaa caggtgttgc aaattcgctg
     4621 ataacgttaa acggcgtcag cacgggaaca ggctccgtga agctcttgag ataattgaga
     4681 aaacctccct tgagcttgta gtgcgtaata agcacaaaaa cgataactgc ccaaccggca
     4741 acaacgttga tgtccgaggt cggcggaaaa a
//
LOCUS       DAWBSW010000157         4752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3174_length_4752_cov_2.330211, whole genome shotgun sequence.
ACCESSION   DAWBSW010000157 DAWBSW010000000
VERSION     DAWBSW010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_4752_cov_2.330211"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4075..4084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1370 a          996 c          925 g         1351 t
ORIGIN      
        1 gtgtgatatg gtatgtcatt agatatgaaa cagaaaaagc caaaaggtca aacaaaattc
       61 ggaaaaaact gccagaaacg agcggttttg aattacacgg ttattgacaa cggagtaaaa
      121 attcgaaagc aaaaggcttt ttatggtaaa acgctgaaag aagcttgtac tctccgtgat
      181 gagtataaac gcaaaataaa caaaggcatc aaacctgatg atgccaaggt atcagtatcg
      241 gactatgtcg ataagtggct tgcattaaag cagaaaaata tgaatgttaa taatgcaaga
      301 gcaaaagata tcaaaaagta catcggccat atgcgaattg gagatgtccg ccgttcagac
      361 attcaattca tgataaatca gttttcagat actaaaacta ttcgtgggaa atacccaggg
      421 aagaaatctg ttgagtctct aagatatgtt tcacatcagg tttttgaata tgcacgactt
      481 gacatgatta ttgaaatgaa cccagtgcaa agtatagata tccccagcta tgagaaaagt
      541 aaggtcagac cgcttacaat gcaggaaatt gataatgttt tgaaccttga acataagtgg
      601 caaaggatat ttatcattct gttacatagc ggactgcgac gtagcgaact gataccgctt
      661 aaatggaagg atattgattg ggataatcag tgcattattg tcaataaatc aacaaatttt
      721 ataagcaatc aagcagtcgt caaatattat actaagaacg aagcaagtaa caggaaagta
      781 tatataacta accaatttaa atcatatttg gatggtttgt acagtgatga ttccattaaa
      841 tacagagata ggaaaagact aaacgaagcg ttaatttgcc cttctttgac tgggaaaatg
      901 ttttcagaaa cgcaatggac aaatatgttg aaaaaatacc acaccgatta tgaactggca
      961 tatggtaatc cgaataatta tcacaaaaat gataaaaggt ttgagctaac gctgccgatt
     1021 cttaacccac atatgtttcg gcatacattt tgttgttcaa tgttttacgc cggaataccg
     1081 gtaaatattg cacaaagaca aatgggacat gagaaagcat caacaaccct tgatatctac
     1141 gcacaatgcg aaaatgatga gactacccga aaagaaattg atgaaaaaat gagtaaagca
     1201 gacacacggt ctccacagga gaaattactt gaagcaatat tcagtgatgg taagtttcca
     1261 acacgattat taaaatgatg ataatttgat gataattatt gagattttca ccacttctgc
     1321 tgtgtgatta tattgaaaaa taagggattt ttatatcagc taagtccttg gctacgaacc
     1381 aaaaggtcag gggttcgaat cccttgcagc gcgccaaaac agaagcaatg cgattttgtg
     1441 ttgcttctgt ttttttattc atggattcga gccctgagag ggtgagcggc gttaagaaaa
     1501 cagtcctgtg gactgttttt agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nntcgcgaag tgtgaggcgg gtactgtcgc catgcgacgg tcgccgagca cgcaaggtgg
     1681 cttgccgcct gtatcccttg cagcgcgcca aaagagaagt gatacttcgg tattgcttct
     1741 ctttttttta tgaatttttg agggattcga accctgagag ggtgagcgac gttaaggaaa
     1801 cagtcctgtg gactgttttt agtcgcgaag tgtgaggcgg gtactgtcgc catgcgacgg
     1861 tcgccgagca cgcaaggcgg cttgccgcct gtatcccttg cagtgcgcca aaagagaagt
     1921 gatacttcgg tattgcttct cttttttatt tttttatgaa tttttcaggg attcgaaccc
     1981 tgagagggtg agcgaattta aaagccgtgt cttgcacgtt cacttcacag ttgcaaaaca
     2041 cggttttgtt ttatttattt ttatcaatca gctcatcaag caacgcagta cagccgtcaa
     2101 ggacatcttt atatgtcact tcgaaatttc tcgaatacca tgggtcggca acatctcccc
     2161 cacgggaggt ataatccatg agcttatgta tctttcggtc ggggtcattg ccaaagatat
     2221 aaagcatatt tcttatgttt gcactgtcca tgccaatgaa caaatcgtac ttgccgtagt
     2281 catttttctg aagctgaatc gccctcttgc cgtcacattt aagtccgtgc tttgcaagct
     2341 cggctcttgc cggcggataa acgggattgc cgattccgtt ccaaatctcc tccgtgcttg
     2401 ttgcagatga agcaatctca aatttatact caatcccctg ctttttcaca aggtctttca
     2461 ttataaattc cgccatcgga ctgcggcaga tattgccgtg gcagacgaac attattctga
     2521 ttttcggtaa cattttttca ccgccgtttg tttaaaattt catttgctcc gaataaattt
     2581 tacataaaag ctttcggcaa tttcaagcag gtacgaatcc aaatacgaaa acgccgtcgt
     2641 cctcatttca tcctccatac cgtagtacgc ttcggcaatc gagcctgtta tagcggcaat
     2701 agtgtcgctg tcgccgccga tagaaattgc gtttcttatc gcatcctcaa agcttaccga
     2761 ctcaaaaaat gcctccaacg cctgaggaac ggactcctga caggtctcat agaagccgta
     2821 agtatcacga atttcatcaa gtgtgaaatc tattttatag aagctgttta tgtaatcctg
     2881 aatctcagcc atgctgtgtc tcgtccttgc aagataaatt gctcccgcaa cggcaacagc
     2941 gccctttatt ccctcgggat gactgtgcgt aacctctgcg gtcgccgccg ccaatgcttc
     3001 acattcatcg agagaattcg cataccatgc aacaggagaa actctcattg ctgagccgtt
     3061 tccgtagctg ttgtacggtt cggtatagtt ttcccttatc cacacgccaa aatgtccgcc
     3121 gtagccgcaa ttcgggtatt tgattccaac ctcatgcatt accgacacaa gctctttctt
     3181 gaattctttg atatcagtca cggcgtcaaa cttcaaaagc gtctttgcaa ccgccaatgt
     3241 cataaccgta tcgtccgtaa aaaagcacct tttgtcgaaa aaattaaaat cctttgactt
     3301 gtgattataa aactcaaatc ttgatccgac aatatcaccg atcattgcac ctatcataat
     3361 aaattcctcc gaaaataaat ttacaagctt ttcaccttgc agcattatta tatataagac
     3421 atgcgccgtc aaggtcgcct gataggtgac attttattta atttctgtcc cattctcaac
     3481 gagactgtca atatcaattt ttgtgttgtg gaaatcggct tcaaaagcct gctttaaatt
     3541 ttgcgcctca aatgaggaaa gcattctgca attataaaat gttgagccat gcagttgatt
     3601 aacatttgcc gataatttaa cgcattcaag ctccgaacag ctaaaaaaag catagccgcc
     3661 tagatattcg acgctgtcgg gaatcgtaat ttgttttaat tcgccgcact caaagaatgc
     3721 actgtcatca atgcagttaa gcctttgagg aagtgctata tccgataacg ctttgcagcc
     3781 gtggaacgca ccgttgtcaa tgcacaaaag cgactgtgga aggctgacct tctcaagcat
     3841 catgcaattc tcgaaacacg cttcgccgat atactgtata ccatcatcaa ccgtaacttc
     3901 ctttaagcta atattcggca ggaactcact tttatcgaat cggcaggtta cgccgatgac
     3961 ctcatgaccg tcaataacgc tcggaatcgt taccgtggaa gagtcaccgc tgtactcggt
     4021 tatgaaaacc tttttgtttt caaagagccg gtatttaaag tcgccgttct ccccnnnnnn
     4081 nnnnccttcg gatacaagga aattttgatt atcctccgtg ccgaaataat aggtcgaaaa
     4141 ataatcgcaa agcgttatat ccggatgact gtacttttcg gcgatgaaca gataatcgtc
     4201 ccatgtaaat ttatcggaaa tttttacgtt ttccttttga aaattcaccg ttgtgatgtc
     4261 ggttgttgtg gactccgttg tcgttttatc atcaacattc tgcacacacg acgtcaggaa
     4321 aattattaaa agcatggaca gaaatacaac aattcttttc attttattac tcctccctat
     4381 ccaatgcaat atccttatcg ttcatccaaa tcataccgtc gccgaaattt attcctgcgc
     4441 ttttaaaatc ggcaacattg atttttgttc cgctcaatgc cgttgtatct atctctgtca
     4501 gcgctctcgg aatattgata tccgccaaat ttttatcacg aaaaaaagcc tctttttcaa
     4561 ttctgtaaac ggtttcgggg agaataactt tttccgtatc ggcacactcg gcaaagcagc
     4621 ctgccgagat ttttctcagc ttcgtctttg aaagatcaag agtcttgagt cttttgcagt
     4681 cataaaacgc cctgtcgccg atttcttcca gcgtcgaagg gaaatttatt tccgtcaggc
     4741 tctccgcaaa tc
//
LOCUS       DAWBSW010000158         4692 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3213_length_4692_cov_2.202070, whole genome shotgun sequence.
ACCESSION   DAWBSW010000158 DAWBSW010000000
VERSION     DAWBSW010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_4692_cov_2.202070"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    788..797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1526..1535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2481..2490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3405..3414
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1407 a          929 c         1034 g         1282 t
ORIGIN      
        1 tcccacaaca ataagccagc aaaagagtat ggcaaagcag cagcgtcttg caccgaagat
       61 cagacgaatt caagaaaaat atgcgggaaa tcagcagaag attcaggaag aaacccagaa
      121 tctttatgcc agagagggct ataatcccat gtcggcaggt tgtctcccgt tgcttattca
      181 gttcccgttt attatcggtc tttacggcgt gctttacaat ccgcttcgct atgcgatcgg
      241 acttgatgct gcaacgtcac agcaattcgt agatgttttc tccaatatgg ttaaggacgg
      301 ggttgttact gtcggttcaa aagttgacag atactatcct ctttatatca ttgagcattt
      361 ctcggaagta atggattata tttcatcaaa aggaattaca accgtttcgg ctgatgcaat
      421 ttccagagtt cagtcattta ttgatgccaa gaggtttatc ttcctcggtc ttgagcttgg
      481 tgttcaaccg caatataagg tgttcaataa gtattggctt attcctatcc tttcgggtgt
      541 tacatcattg atttccgcga ttgtcacgca gttgcagcag aaaaaaacaa atcctgccgc
      601 aaacacaccc ggaatgggtt gtacaatgct tatgatgccg gcaatgtcgg tttggtttgc
      661 attcatgttc ccgagcggaa tcggagtata ctggattgcg tcaaatatat tcgcattttt
      721 ccagacattt attatgaaaa aattcatgtc tccgcagaag aacattgcga agcttatggt
      781 taaagagnnn nnnnnnnttg aaagacgttc gagagagaac agcattaagc gtatcaaaaa
      841 catctgaaaa ttatgaaaat aacactgtaa agtgtagatc tacagaggtg aaaaccgttg
      901 attaaagaag cattcggaaa gggcgcaacc acagaagctg caatcgaagc cgctaaaagt
      961 gagcttggag caccgtggga cgccgatgta caaatagaag ttttgaaaac agccaagaaa
     1021 aagactctcg gtcttttcgg cggttctctt gccgaggtta gagcttacta tgaggttccg
     1081 gacgaggtta aaaagcccga gccgaagcct gctgttaaga atcagaagct ggcaaagccg
     1141 gagaaaaaag cagaggctaa aaagcccgaa gccaagaaag aagctgctcc caaaaagcaa
     1201 gaagctgtca aggagacgga aaaaaccgtc ggcgaagctt caaaggcagc cgaagcttat
     1261 ataaaggctg ttcttgaagg tatgggagta aaggacgctg aagttacttc gtgtgaagac
     1321 gatgaaagcg tttatgtaca gcttgactgc ggtaacgact acggctatgt tatcggcaga
     1381 aggggagaaa ccctcgatgc aattcagtat cttacaaggc ttgttataaa caaaggtaag
     1441 gataattata agcgtgtttc aatcaacgca ggcaattaca gagaaaagag agaggtaact
     1501 ctccgtgagc ttgcaaagaa gaacgnnnnn nnnnncaaga gtcaagaaat acggcagaaa
     1561 tgtatgtctt gatccgatga atccgtatga gagacgtatt attcatacaa caattcagga
     1621 aatagagggc gtcgattctc attcgatagg ctcggaaagt gacagaagag tagttattac
     1681 tctcgccgag ggatttaagg caaccaatcc gtcgaacggc agaggtcgca gaggggatta
     1741 cagaagacac gacaaccgtt cgcagtcaag ggaacagcag gcacctgcga gagcgccgcg
     1801 ttccgatctt gagggatcgc tttacggcaa gattgagcca aaagcaaaag aggaataatc
     1861 attaaaagaa gcggacaata taattatgtt gttcgctttt cgtcgttctc ccgaggtttt
     1921 attttgcggg cttcggattc gtttaaatgg agttgatttg atggataaca aagaaagaaa
     1981 agacactttg gaaaacatgg atgaattcaa tcagaagtat aaccactatc tttccgaaga
     2041 tgaggaaact caaattgttc ccgttcccca ctttgaaaaa aacgacggga ttgaagatgt
     2101 cagctcgtac agtgaggatg ttaatgcaga ggatcccgat tttgatgcag acagtgacga
     2161 tgagccgact ttggaaaaga aagagaattt cttcacaaag aataagtaca agaacacaaa
     2221 aataattgcc ctttgccttg taattgtttt cctgctcggc atcggcgccg gtttagtttg
     2281 gatctacgtt tcaacaaaga gcaagggcta cggcgacgac ggaattgact ataacaaaat
     2341 tgacaaaaat tatcttgttg atgataatac tcagtttaag gttatgggag atattgatgc
     2401 cgactcgctc aacgctttcc tctatcagtg ggcaaacaac ggcggagata agatgtcgaa
     2461 caagaatatt ataaatgttc nnnnnnnnnn aatttaatct ttgcgactcg cagatactta
     2521 tttccatcga caagaaaaac gaaaagatta caatggtttc tttccttcga gacagctgga
     2581 catatatcag aatgcccaag gaggacggaa cgtattatga cgaatacgaa aagataaatg
     2641 ccgcttatca cggcggtccg aaaactcttt tacagaccat tgaaaacaat tataaaatta
     2701 aaattgacca gtatatagtg gttgacttca agtcattccc gaaggtaatt gatgcaatcg
     2761 gcggggtaac ggtcgatgtt caggactatg aggcagatta tatcagaaga acgtcgtctc
     2821 agactaattt tccttatggc acggcaaagt tgaacggcaa gcaggcgctt atctacagcc
     2881 gaatccgtca ttgtgacgac gacagcgacc tcagccgtac aagacgtcag agatctgtta
     2941 tcaagtcgct catcaaccag gcaaaaaaag cctctaacgg ccagcttgtt aacgcctata
     3001 agcaggtaac gggttatctc aggaccggat atacacagaa agaggttctt tctcttatcg
     3061 caacggctta ttcgcataat tggatggatt ttgaaatcaa ggaatacatt atgcccaatg
     3121 aagattatgt tgaaagaatc ggcggttaca tcaattcttc aacatgggct tggacagttg
     3181 actatcctct ctgtgctcag agacttcaaa aacttcttta cggtaaaacg aacattgtcc
     3241 ttgccgcaga ccgcgtcagc ccgcttgact cggtatcaaa cagacgagac actactacgg
     3301 acagcagctc gggcaattca tcgtcgggag attattcaaa cgataataac gataacggct
     3361 atgttgagcc ggatgtcccc gatattgaac cggtaactga ggaannnnnn nnnnttgatg
     3421 caatgccgtt ttgttttctt caagatccgg aacaaggacg tcctgaccgt caatcatttc
     3481 gctcgaataa ccgtcatcgg tcggaattct gaactgatcg ttgccgtatt tcagcaaggt
     3541 tattgctccg aaagctttta aagtcatctt gaccggtgaa atatccgttg taataagagg
     3601 aagtacatcg ctcataactg agagcgtagt aatcggattc tgagaaacta tctttttaaa
     3661 tatggaattc attaccttac gctgacgctg agttctgtta aagtcggaat caagatatct
     3721 gattctgcaa taaatcagag cataatctcc gtcgagaata ttttctcccg aatgtacatt
     3781 tgcatggctt gtctcattga tgaaatcggc ttcgtcttcg gtaatgtcaa cgcttactcc
     3841 gccgagaccg tcgataagct tttcaaacat ctcaaagtca accatcacat aatcgtttat
     3901 cgaaacgccg aaattctcgt aaattgtttt cagcaaaagc tccgctccgc cgtaggaata
     3961 agctgcatta agccgcccgt cgtcatatcc ggggattgaa acccacaagt cacgcatgaa
     4021 tgatgtcatt ttgattttct gatgctcttt atctattgta acgagaatca ttgagtcgga
     4081 gcgttgagac gttccgtccg atttatcgca gccgatgaga agaacgttat aataattcgg
     4141 atcctcggtg aaatttgagt tgctgctttg aacataattt gtttttgcgc acattgaata
     4201 aacataaccg aagctgccga cgggaataag ggcaatgagt aaaaggaaga taataaacag
     4261 tgttccgcag ccgcaaccgc agcctttttt ctttttcttc tttttctttt cgtttttgct
     4321 ttttttgtct gagccttttc cgctatcctg cctcggagga atctctccgt tttggtagct
     4381 ctcaaaggtt ccgagccgct cctgattttt aattatttct tcgtattttt gctgattaag
     4441 tctgtcttga aagtcttttt cggactgagt gatcggtatt tcacccgtat cgtccctctt
     4501 tttatagttt ttaaaataaa tatcgctcat tttttcacct tctaatgcaa tattttacta
     4561 tatcttttct ttttttacaa gagttttgaa aaatatatga gcgttttaat tgacttttat
     4621 tacattttaa attaaaatat atgggtaatc gttgattatc atacaagcag gttgggcagt
     4681 tgcatgcatt tt
//
LOCUS       DAWBSW010000159         4673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3232_length_4673_cov_2.493504, whole genome shotgun sequence.
ACCESSION   DAWBSW010000159 DAWBSW010000000
VERSION     DAWBSW010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_4673_cov_2.493504"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1228 a         1039 c         1180 g         1226 t
ORIGIN      
        1 gaatagaaca gttcctttga gcgcttcttg cttattttac gaaagacata atgaccgaaa
       61 agtccggcgg cctgcttcgg aagaaatctt ataatcgacg ggtgacgctt caggcagaaa
      121 agccagaatt cgctgcccga ttcgccgtca aaaaccgtct tgtcaaaatc atagagatca
      181 gcgagcatca gaaatcaaca tcctttttgc cgtcaaggcc gttgtagaca atttcaaatt
      241 cgccgctgcc gtcaagcgtg agctttgtaa gcttgccgtc tgcccattcg atatctacct
      301 taactccgcc ctttgccgtc aagccgtaaa tatgaccgtt tgcccattcg tcggggaggg
      361 cggggagtat aatgatacgg ttgccgaagc tttgcatgag cgcctgtgcg atacctgccg
      421 ttgcgccgaa gttaccgtca atctggaacg gcggatgagc gtcaaacata ttcgggaatg
      481 tgccgccgcg acctctcttg tatttgaccg gaatatagcg aagctgatta ttgatgagct
      541 tgatgacctt gttcttgtca cgcagtcttg cccagaagtt gattttccag cccaagctcc
      601 agcccgtgcc gccgtcgccc ctgtttttga gcgtattctt ggcataggaa gccagatcgg
      661 gtgtgcgctc aacgtcaata agatttgcag gatggagagc gtagaggtgg gaaacgtggc
      721 ggtgatgctt atcaaagccc ttttcgtcat agtaccattc cataagtcca ccgtccttgc
      781 cttgcttaaa gggaagcaga cgggggagcg catcttcaat tctgctgctt atctcatctc
      841 taatgccgag gacatcgtat gcagagagaa gatttgtaaa aagatctttg acaatttcca
      901 tggtcatgta tgttgtcttg ctgacggagg tttccttacc gtttaccatg tactcgttct
      961 cgggcgaggt cgagggacag agaataagat atccgttgcc gtcgtcgatg agcatatcaa
     1021 ggcagaagcg tgcgttttct tccattatcg gaactgctgt tttctcaaga taattcttat
     1081 cgagcgtata ctcataatat tcataaaggt gacggcagaa ccatgctcct gccatcggcc
     1141 agaatgacca aacgggattt cctcccgagg gtgtagttat cctccaaagg tcaacgttgt
     1201 gatgacagca tgtgccgtcg acgccgtaga acagctttgc ggtctccttg cccgactccg
     1261 caatttcttc aatgaattta atgagcggca tattcatctc gggcattgag cacatcatag
     1321 tcggccagta gttcatctcg gtgttaatat ttaccgtata gtttgaacgc cacggagagc
     1381 acattgtaaa tgtccagatt ccctgcaatg tcatggcctg tgagccttca cgggaggcgg
     1441 aaatagtgag ataacgtccg taattgaaca gcaggcagta aagattattg tcattcttcc
     1501 tgctgtggag aaagtcgtga attctgcggt ctgttgggac attgtccttt ttgctctcac
     1561 cgaggtcgat agaaacacgg ttgaaatacg gactgacatc atttatatga tcagccttga
     1621 ccgcttcaaa gcccttgctt atggcattgt caagacgtga aacgcagatg ctcttatagt
     1681 cctttagtga ctgagtaacg ggtttgttcc agccctcaaa attatcttcc gcagtcaggt
     1741 aaatcgtcgc atatgttgcg tcggtgacgt gaaggtgatt tttattcgcc ttgaccttgc
     1801 cgtcggtgtc tatcttgaat gcgcagcgga agctgatgcc ctgtctctcg ccgtcctcat
     1861 atgtgcagat ggggttgccg ccattttctt caatatgtgt cggagcgata ccgtcaagga
     1921 aatatgtatt gccgtccgtt gtaacctcat agcgaagctc acagtcaacc atagccttga
     1981 aatcaagctt gtttttgccc tccgcggtca ttctgtagac aagtaccttg tcgggaaagc
     2041 tgatgaagct ctcacgggta tagttcgtat cgcttgaatt gtaaccgacc gttgcgattc
     2101 cggtttcaag gtcaaggtca cggatataat ctctcggaca catattcttc attgaagtga
     2161 ttatgagttt tccgagctga agataccact ccgacggatc gccgtggaag ttctcttcga
     2221 tataatccct tgcttcgatc ttcttatcct caaggatgag ctgacgagcc ttcttgaaat
     2281 gctcaaccct gttttcaacc ttgtagccgt ggcatgaacc cgaccaaagc gtatcaagat
     2341 tgaggccgag tgtttcagtc tcaacaccgc cgaacaccat tgcgccgagg ctgccgtttc
     2401 ctataggcaa agcttcgacc catgcggttg cagggtggct gtaccataaa tattgcgacc
     2461 cttttttcaa ggaaataacc tccaaaataa tatttaagca acaccttgat tgtatggcat
     2521 tgcctttacg gtaaaagtat aactgattgt tcacattaaa gtcaatcaat tatcttgcat
     2581 ttttcataaa tttattataa aatagcttta attggaatta aaaagtgttt ttaggagaag
     2641 acaatgtcag aaaagatttt aattatcggt gccggtgcgg caggacttat ggcggcaggt
     2701 acaatatcgg gcaggggcaa ggatgttgct attcttgaac gcaacgataa gccggcaagg
     2761 aaggtcgcaa taacgggtaa gggcagatgc aatgtcacga ataactgcaa ggagctttcg
     2821 gagcttattg ccaatgttcc gaccaacggc agattccttt acagtgcgtt ttcaaatttc
     2881 atgccgcagg acacaataga ccttatcgag gacatgggcg ttccgcttaa atgcgagaga
     2941 ggaaacagag tttttccgtg ctcggataag gcgacggata ttgttgatgc gcttgttcat
     3001 tacggaacgg acgacggagc acagataatt cacggcagag ccaagaagct tattgttaag
     3061 gacggcgtgc tcaagggagt acgcaccttt gacggcgagg agattgccgc agataaagtc
     3121 attattgcga cgggcggcaa gtcatatccg ctgacaggct caacgggaga cggctacgag
     3181 cttgcaaggc aggcaggtca tacgataatc gaaccgaagc cgtcgcttgt gccgcttaat
     3241 gcacacgagg gcttctgcac agatcttatg ggtctttcgc tgagaaatgt cacgatgaag
     3301 gtcattgaca cgaagaagaa aaataaaatc atatatgatg atttcggcga gatgcttttc
     3361 actcatttcg gagtttcggg tccgatggtt ctttcggcaa gctcgagaat ccgtgacatg
     3421 gagcctggca gatatattat ttcgatcgac cttaaaccgg cgctcaccga agaacagctc
     3481 gatgcacgaa ttctcaggga tttttcggag aatactaata aggatttcat caattcactc
     3541 ggtgcgcttc tgccgagaaa gctgattccg gttacagtca agctaagcgg aataagggcg
     3601 aacgaaaagg ttaatcagat aaccaaggag caaaggcata agttggttac gcttctcaag
     3661 aattttaagg ttacgatcaa gagcttccgt ccgattgatg aggcgatcgt tacctcgggc
     3721 ggcgtgaaag tctcggagat cgacccgaaa accatgcagt caaagcttct cccggggctt
     3781 tactttgcag gtgaggttat tgacgttgac gcatacacgg gcggatttaa tttacaaatt
     3841 gctttctcaa ccggacgatt agccggtgaa aatgtataac ggaggtttat tttatggctg
     3901 taaatattgc aattgacggt cctgcaggcg caggaaagag cagcacggca aagcttgtcg
     3961 caaagaagct cggctacatc tatgttgaca cgggtgcgct ttacagaacg gttggtcttt
     4021 attctataag aaagggtatt gacacaaaga atgccgaaaa ggttatcgca actctgcccg
     4081 acgtcaaggt ggagcttaaa ttcgttgacg gagcccagca tgtttttctc aacggcgagg
     4141 acgtttcgga ggctatcaga actcccgagg cttcaatggg tgcttcgaat gtttcggcaa
     4201 tcccgaaggt cagggaattt ctctttgatt tgcagagaaa aattgccgcc gagaacgact
     4261 gcataatgga cggcagagat atcggaacgg ttgttttacc gaatgccgac gttaaaattt
     4321 ttctcacgac ctcggttgag gagagagcga acagacgcta taaggaaatg ctcgaaaaag
     4381 gcgaagaagc tgattataac gatattttag aggacatcaa aaaacgcgac tatcaggaca
     4441 gccacagaga aattgcaccg ctcaagcagg cggaggacgc gatttatgtc gataacggcg
     4501 gctataacct tgaagacggc gcagaagatc ttctcagaat tatcaaagag catttgaagt
     4561 gatgcattgt atctcggtgc tcattgtatg gcggcaagaa aaagtcagcg gaaattgacc
     4621 aaggcttccc cttgagggga agctccgtcg taagacggtg atgaggtgga aag
//
LOCUS       DAWBSW010000160         4658 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3240_length_4658_cov_2.622855, whole genome shotgun sequence.
ACCESSION   DAWBSW010000160 DAWBSW010000000
VERSION     DAWBSW010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_4658_cov_2.622855"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1407 a         1024 c          979 g         1248 t
ORIGIN      
        1 gtctaaaatc gataaactac agaaagaatc accgcatctg gctttattat gatttttata
       61 cggtagaaaa ggataacggt tattatcaat ttgccaatga ccttaagcat gatgacggaa
      121 tagaaagata ttatgttctc acccatgaat ataaagacct tgacagtgta ttcactccgg
      181 agcaaaagaa gcatcttgta aaagcaggct caaaggagca cagactttta tttatatgtg
      241 ccgaaagagt ttttacggct tattacggtc ttgcaccaat caatccgttc gaaacaccga
      301 agatgaaata tcagtatgag gatcttttgc gattccgtac tatttatctt cagcacggtg
      361 ttttgcacgc atcactccga cttcttaact ccgaggaacg cagccaggcg gaggaaattg
      421 ttgtttcttc gccgtttgaa attaagaatt acatgaataa ttacgcttat gaggagcgtg
      481 aactaatccc gaccggtatg gcaaggtatg accatattga aagaaaccac gagccgaaga
      541 acagaatttt atttgctccg tcatggagaa aatatcttac aaccgaggtc ggcgcttcaa
      601 actgggaggc aaatgttgaa aaaattcaga aatctgatta ctataagaac ttctatgcct
      661 tcctctcgga caaggagctt cataaaaagc ttgaagacaa caaggtctac cttgatatta
      721 agcttcaccc gataatcgcc aaccttaaaa acctgttcaa tattgattgc gaatatatca
      781 atatggtcgg cgacgtagat gttgccgatt acaaggcgtt cgtaacagac ttttcgtcat
      841 ttgtttttga ctttgcttat cttaacagac cgatcatgta tttcgttccg gattatcctc
      901 agtttaagtc cggcatgaat cattaccgtg atcttgatct cccgtttgaa gacgctttcg
      961 gtcatatgtt tacggatgct cacaaagctg ccgaatgtct ttgcggaatt attgaccgcg
     1021 attttgttcc cgaaaacccg ttcagagaga gaatgaagaa tttttatttt gattttgacg
     1081 gcaattgcgc cgaaaatctt tataagttcg ttgttgatca ggataaagca ttttcagaat
     1141 aatgttatat tataattaaa agcataaaag ggctgttgca aatcattaaa tgatgagtga
     1201 ctgccctttt taaatttatt cggtgagata ccttttccga agcgacagct tgtaacttta
     1261 tttttttaaa ccaaatccaa acgtaaatat aaataaattg taaaatttaa ttcataaagc
     1321 aaaaaaatta ttgtttaggt cttgtaaaaa agatgtaaat gtattaaaat actattgtta
     1381 tgctgacttg ttttcggcag atatttcatt aagtaaagga tgaatacaat ggctaacgaa
     1441 aagtcaaagg cagagcagta ccgcgacgaa agaaaagctc gtattgctaa aactgccaaa
     1501 aagaatgcgc gcagcatgga agcaagaaac accgcgaaaa aggttgcaag taaggttatt
     1561 tccattgtgc tttgcgctgt aatcgtttta ggcgtagtag cattctctct caactactac
     1621 ggcgcacttc agagagttat caagctcggt tcggtcggct cggagcagtc cgttacaatc
     1681 gccgaatacg agtattacta catgcgtgct tataaccagg ttcgctatca ggctcagtat
     1741 tatcagtact attaccagac aagcaacggt tatgacctct ctctcactcc cgaggagcag
     1801 actcagacaa ccaaggactc cgaaggtaat gagattacat gggcagagaa gcttcagaag
     1861 gatacgattg agctgattca gctccacaag gcatattata acgaagctct caagaagggt
     1921 ctcaagctta caaaggctga tgaggcttac attgataagc agatcgagga tcttcgcgat
     1981 gaggctaaga gcgcaggttc aaactcaagc tcagacagca aaaacaaggt aacatattca
     2041 ctcaacgcat accttcgtaa ggtttacggc ggttcaatca atgagagatt tctccgcaag
     2101 caactcaaga ttcaggttct tgctcagaaa tatttgaccg agagaacagc cgaaatcgca
     2161 aaaacctacg accagaagga tatcgatgcg gagtacaaga aggacacggc agcatatgat
     2221 tttgtaacct tcagagctta taccttcaag acaaccgagc tcactaagga aaataacgag
     2281 acagatgacg ctctcaaggc tcgtcagacg aaggctaacg ctgaggtaaa gaagaacgct
     2341 aacgacttct acaatgccgt tacaaacgag gcaaccttcg ccgccaaggc aaaggagctc
     2401 aacaaggata cggctgatta caatgttgac gaggaaacaa agtattcaat gctcaagagc
     2461 accgctcagc aaagcttctc agaggatgcg gctaagtggc tcttcgacag ctcgacaaag
     2521 gaaggcagca agaagctctt ctctgatgaa gaaaaaggta agtacatagt tgtccttgcc
     2581 gtaaagaagc cgcatcagga gcagacagtt acagcacgcc atattctttt ccttacaaca
     2641 gatcagagca caggcagtga tctctccgag gaagaaattg caaagaagaa gaccgaggct
     2701 gaggacgttc tcaagaagtt caacgagggt gataagtcag aggacagctt tgcagctctt
     2761 gcaaatgagt acaacgagga cacaggctca acttcaaacg gcggccttta tgaacacatc
     2821 tatcccggtc agatggtaac gcagttcaat gattgggtgt ttgatgcaaa ccgcaaggca
     2881 ggcgacgtcg agatggttaa gactcagtac ggctatcaca tcatctactt cgtagctaag
     2941 gacggcactg attactatga ttcagcgatc cgttcttcaa aggcaagtga ggatattgag
     3001 acagagacca aggaacttca ggacggcgac acatataagg catatatcgg tccgcgtctt
     3061 tcaaaatacg cattgaatag agtttccaag aagatcaaat atcttgttga actcagcaag
     3121 gctaactcct cttcctcagc aagctaagta atacaataac atttgaccgc ttcattatga
     3181 ggcggtcttt tttgataaaa ttataatgac ggacataatt gcccgccata ccataaaaaa
     3241 gctgtgacag atcattccgt cacagctatg tttttattta attgagattt ccgacatgat
     3301 cgggcaatga tccgatggga atgctccgtt cggctgaagc ggtcgctgat gaaaataaaa
     3361 tcaattacat cctccgactc gttatggtga aaatacgggt gaaatgtcgg ataattctcc
     3421 gtttcttcag caacaaactt gctgtccctg aatattccgc cctcgaccat atcaacgtag
     3481 caatccgtac cctcggaaat gttgaaatct cctgagatta caacgggaca atcaagtttt
     3541 gcggcgaact catttataag ccttatgccg ttctttctcg cttcctcgct tctgtggtca
     3601 agatgagtgt tcatggcaac aaatgtcttg ccgctctcgt tttcggtgag cttcgcccac
     3661 gaagcgattc ttgtgcaagc gctattccac gatttcgacg ggacgtccgg tgtttcgctt
     3721 atccaaaacc agcccttgtc aacaagagtg aatttatctt ttttgtaaaa cacaggtgaa
     3781 aactcaccct cgtccttgcc gtcatctctg ccgactccga cgaaatcata ttcgtccatg
     3841 ctctcgcgga aaatgcccat ccagccgata tgtgcttcct ggattccgaa aatgtccggc
     3901 atataatttc tgacggttgc tataacgtca gcgtctctgt ctaaccacga gtgcccctca
     3961 cagccgtagc agagtacatt aaaactcata atcttcaatc tgcctgctcc tccctattca
     4021 ttatctgata actgagagtc ataagactgt cattaagatg aacgttttcg accgttactc
     4081 ccggataatt cgacgcaatg tcgtaatggc tgaaattcca gaaatttgtt attccgagat
     4141 taacaagcct ttctacaatc gcaggagcag cctcggtagg cacgcaaagc acggccacct
     4201 tcggctcaac ctctttgcaa tagacctcca aatcctcaac aggatacacg gaaagtccct
     4261 ttattctgat gtccttcatt ttatctgcgt tgctgtcgaa aagtccgaca agctcaaatc
     4321 ctctgtcctc aaaggtcata tgagaagcaa ttgctctgcc gagattgccg caaccgaaga
     4381 gaattgtttt gaagccgttt tgaacaccga gaattgtgcc gatttcattt tgaagctgtt
     4441 ccacattgta gccgtagccc tgctgaccga atccgccgaa gcagttaagg tcctgtcgaa
     4501 tctgcgatgc ggtcaagccc atgcgctgag acagctcttt tgatgaaatt cttgttatat
     4561 tatttttttt aagttcgccg aggaatctgt aatatcgggg caaacgctta attacggaca
     4621 ttgaaaccga tttcttttcc tttgccacag ttattacc
//
LOCUS       DAWBSW010000161         4632 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3268_length_4632_cov_2.271575, whole genome shotgun sequence.
ACCESSION   DAWBSW010000161 DAWBSW010000000
VERSION     DAWBSW010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_4632_cov_2.271575"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4151..4160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1246 a         1100 c         1045 g         1231 t
ORIGIN      
        1 aaatgcaatg caaatcagga atgagctaag cagcatttcc gacggaatta tggactcgtg
       61 ggaaaagcga aacacaagtc aggatataat aagccaaaag cagagtgatg cgacactcgg
      121 tcttgaaaga atttatgaca ccgagaagga tgaaatatat atggcgaaaa acggctggag
      181 tgacacgaac aaggacgaac gttttcagct tgttaccgat gacagtatgt atcttaaggc
      241 aacctccggc acaattgagt aattaaaaaa ttatatttaa gcctcaaatt acaaaaaact
      301 ctgtaatttg aggcttttgt cttgcgctcg aaaaggcgat atgatacaat gagctgaaca
      361 cgataaattc ggaggaaaaa tcatggatta tttgaaatat gttaacataa aacagggcac
      421 aaattccgtc agacgttttt caaacggaaa tacactgccc cttgttcaga tcccgttcgc
      481 aatggcaggc tattcggctc agaccgaata tgacggaggc tggttctaca acccgactca
      541 ccgcagtctt gagggcataa ggctcactca tcagccgagc ccgtggattt ccgactacgg
      601 aacttttctt ttcactccgc aaacgggtga agtcagggac tctgtcggca gcgcatggtc
      661 gagctaccgt ccggaggacg cagttatgac accgacatat atgaaattcc gttttcttcg
      721 ttcgaggact gattttgaac ttactcccgc tgagcgaagc tgtaaaataa ggctcaccta
      781 tcccgagggc agaactccgt acttcactgt tttccccgtt ttcggcagct atacttttga
      841 ttttgatttt gaaaacaacc gtctgctttg cacaaccgac ggtgcgagag gagcttttca
      901 cgagggcttc aaaacatatc ttattgttca gttcaaatca aagcttgacc cggagaaatc
      961 ctacaaaaag gacggcgagt ttattcatgc cgcactttgt gacacatcgg ttgaagccga
     1021 tattgcctcg tcatacataa gttttgagca ggcacttgaa aacatgaagc aggactgcga
     1081 aggcaaaagc ttcaacgagc ttcgttcaaa gggtaatgac atatggaacg aatatctatc
     1141 gagaatcgag attgaccctg ttgacgaaaa gcaaatgaaa acattctatt cctgtatgta
     1201 cagaacggga ctttttccgc acaaggcata tgaaattgac aaggatggca acaagcttca
     1261 ttattgcccg tttgacggca gcacaaaaaa cggtgtgaga tacaccgata acggtttttg
     1321 ggacacatac agaacggaat ttccgctttt tgcaatgatt gctaagaaag aatatgccga
     1381 gatgctcgaa ggatttatcg ttgactataa agagggcggc tggcttccga gatggccgtc
     1441 aatcggtgag agaggctgta tgccctccac cctgcttgat gcaattatag ccgatgccgc
     1501 agttaaggga atcatcggcg gtgagcttct tgaaacggcg cttgaaggta tgctccgtca
     1561 tgcaaatcac gattcacacc atcccgacct tggcagaaca ggagcggaag catatgtaaa
     1621 atacggctac gttccggcag atattattga caacaactgc gtcaacctca cccttgatgc
     1681 ggcatacggc gactattgca tagcacaggt tgcgaaggtg ctcggcagga atgaaatcgt
     1741 tgacgaatac atgaagcgtt caaaaaatta caaaaatctt ttcgaccccg aatcaggctt
     1801 tatgagagca aaaacgaccg acggaaaatt caaagaaccg tttgactgct tcgactgggc
     1861 ggacgaatac accgagggca gcgcatggca gtcatcgttt tccgttccgc acgatattga
     1921 cggacttgcc gagctttacg gcggcaagga caagctgatt gaaaagcttg acgagctttt
     1981 cgcaactccg ccgatttatt gtacgagcgg ctacggctct gaaattcacg aaatggcgga
     2041 gatggcggcg gtcgatttcg gtcagtgtgc aatttccaat cagccgagct tccatctccc
     2101 ctatttgttt gccgctctcg gtgagaaaga aaagagcgaa tattgggtca agaagctctg
     2161 cgaggaggcg ttcagctaca cggacgacgg cttccccgga gatgaggaca acggcgcaac
     2221 ggcggcatgg tatgtgcttt ccacactcgg aatttacgac atctgcccgg gcaaaaatga
     2281 atatataaaa atcaaaaagc tttgcaagag tgcaaaaatt ctcggcaaag aaatataaaa
     2341 tcatactttt tcggagggtt ggtttccgct ccacggtttt tataatattt ctgcatcatg
     2401 tacaatatcg tataaaccaa ctgccgcaac attcagttgc ggcagtgctt ttatttattc
     2461 acttattcac tgtaaagcgg aaacctttcg caaagggact gaacctcaga gaggaccctg
     2521 tccttatttc cgtcaaagtc attgatgata tcgcttatcc aacctgcgat aagctccatt
     2581 tcaggctcct tgaagcctct tgttgtaacg gcaggtgttc ctattctcag tccgcttgta
     2641 acaaacggac tttgcggatc gtttggaatc gtattcttgt ttgcggtgat gcgaacctca
     2701 tcaagtctgt gctcaagctc cttgcccgtg atgcccttat tgcgaaggtc aagcagcata
     2761 agatggttgt ccgttccgcc ggaaacaata tcaattccgt gcttcataag ctcatctgca
     2821 agaactgcgg cgtttttaac aacctgcttt tgatagtttt tgaactcctc tgtcaatgct
     2881 tcaccgagtg caaccgcctt tgcggcgata atatgcataa gcggtccgcc ctgcgttccc
     2941 gggaaaacgg ccttgtcaat ctgctttgca aactgttctt tacaaaggat catgccgccc
     3001 ctcggtcctc tgagagtctt atgagtggtg gtggttacga tatcggcata cggcacgggc
     3061 gaaggatgaa ctcctgcggc aaccagtccc gcaatatgag ccatatcaac catgagatat
     3121 gcgccgacct tatcggcaat ttcacggcag cgagggaagt cgattactct tgaatatgcg
     3181 cttgctccgg caacaataag ctttggctta cattccagag cgattcgctc catctcgtca
     3241 taatcgattc ttgcagttgc ttcatcaacg ccgtaaggta caacgttaaa atacttgccc
     3301 gaaatgttta cgggagaacc gtgtgaaaga tgtccgccct gctgaagatt cattcccatg
     3361 acggtatcgc cgggattaag cagtgcaaaa aatacggcaa ggtttgcatt tgctccgctg
     3421 tgcggctgaa cattgacatg ctccgctttg aaaagctcct tagctctgtt gcgggcgatt
     3481 tcttcaacaa catcgacgca ctgacagccg ccgtaatacc tctttgacgg atagccttcg
     3541 gcatatttat ttgtcaaaac gcttcccgca gcaagtaaga ccgcctttga gactatgttc
     3601 tcagaagcga taagctcaat gttgcgctgc tggcgttcaa gctcgctttc gcatgccgaa
     3661 taaacctcat tgtcataagc ggaaagttca tcaaaaaatt tcatataccg tcctccgttt
     3721 tctaaaaata ttctatttat aatcccggtc ggtttctcaa ccggcattat cacattcctc
     3781 ggcaggtgtc tgcggcgttg cgctgaaata cttttcaccg ggttcaacat ccactcccct
     3841 gattttcata agctcggaaa atgtaacaaa cttatatccc tgctttgcaa gctcggggat
     3901 aagccttgca acggcatctg ccgtcgcagg ataaagatca tgcataagaa caatgcttcc
     3961 gtcaaaaaca ttgtccatga cggtctgata atccttgtca gcgtttctgt aatcccagtc
     4021 gcgcgagtca acgctccaca aaaccatcgg cataccgaca ttcgctttga ccgtgtcgtt
     4081 cgcagcaccc tcgggaggtc tgagaacctt gacctttttg ccggtatact ttgatatggc
     4141 atcgcatgat nnnnnnnnnn tttccttttg catttcggga acagaaagtc tgttgagatt
     4201 aacatgagaa tacgtatgag tgcctatctc acagcccatt tttgaggctc ttattatttc
     4261 atcggaatat gtatccgctc tgtcgccgac aacaaagaaa gtcgccctgc cgttatattt
     4321 ttccaaggta tcgagaattt tattcgtgac cggagcataa ggaccgtcat cgaaggtaaa
     4381 gcacacaagc ttatccgttg ctttcgggag cttccattca cccttttcct ccgcctcggt
     4441 tgttttctcg gttgttatct ccgtcgtttt ctccgtcgtt tttaaatcat tggaatctgt
     4501 ttgtttgtct gatgtcgttt cgctcgtttt gttctgtgca tcatgtcttt cctgcatcat
     4561 ttttccgctg aatccgtaca cggaaacaac aagaatagcc acaaatgcta ccaaaaaaac
     4621 cgttttacct at
//
LOCUS       DAWBSW010000162         4567 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3312_length_4567_cov_2.396941, whole genome shotgun sequence.
ACCESSION   DAWBSW010000162 DAWBSW010000000
VERSION     DAWBSW010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_4567_cov_2.396941"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1295..1304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3801..3810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1295 a          954 c         1104 g         1194 t
ORIGIN      
        1 agcagattct ggcgctacac tcaggacgaa tatctcgatt taatgaataa atttgagaaa
       61 aaggatattc cgctgacggt ggcatgtatt gatatggact ggcactgggt tgatgttgaa
      121 aagcgcttcg gcgataaagc caaggactac ccaaagccaa aaaatccgat tgcatatatt
      181 accggctgct tttggaatcc cggctggacg ggttacagtt ggaatacgga gcttttcccc
      241 gatcatgtca ggttccttaa aatgcttcac gaaaagggac ttaaaacaaa tctcaatatt
      301 catccggcgc agggagtccg tttttttgag gacagctacg ttgaatttgc aaagcacatg
      361 ggtattgatg aaaaaaacaa agagccgatt gattttgatt ttaccgataa aagcttcata
      421 gagggatatt tcaaatatat caattctcct atggaaaaag agggagtcga tttctggtgg
      481 atagactggc agcagggaag caggtcgaaa attgaagggc ttgatcctct ttggcttctc
      541 aatcactatt attgccttga caggcagtca aagggtcttc gtccgctgac actttcgcgt
      601 ttcgcaggct tcggctcgca tcgttatccg ctcggatttt cgggcgatac ggccatcaaa
      661 tggtctgtgc ttgattttca gccgtatttt acggcgactg ccgctaatgc aggctactgc
      721 tggtggagtc acgatatcgg cggacacaat ttcggtaagc tcagtgatga gctatatctc
      781 aggtgggttc aattcggggt tttcagcccg ataatgcgct ttcattcggc caatgtcgct
      841 cgcaaaagca aggaaccgtg gaaattcggc ggtgaagcgg aaagaatcac aaagaaattt
      901 ttgcgtttaa ggcatcgcat gataccctat acatatacaa tgaacaggct ggttgaaaaa
      961 gagggcaggc cgttgatcat gcctatgtac tatgcttatc cgaaccgtga tgaggcatac
     1021 tcggtcggca atcaatactt tttcggaaca cagcttatag ctgcaccgat aacgcacaag
     1081 gtaaacaaag agactcacat ggcatctgtt gagatttggc ttcccgatgg aagatacacc
     1141 gacattttca cgggcgaggt ttaccgtggc ggaaaaacgg ttgttcaaag agatattacg
     1201 acaattccgg tgattgcgcc tgccggagcg attattcctc ttgacggcag aacaagcgga
     1261 aatgactgcg gaaatcccga gtctcttgaa atccnnnnnn nnnnggcaat ggctcgttca
     1321 agctctacga ggatgacgga gaaaccctcg catacaaaga cggacactgt gtcgagaccg
     1381 agtttaagct tgaaaaaaac gtgtcggagc ttaaatttac cgtcagtcct gccgacggtg
     1441 acctgtcatt gataccgagc ggcagaaaat atattttcag cttccgtgat gttgcggcgg
     1501 cggagaaaat atcggttgtt tcaaacggag aagcggttga ttatacggca gaaaaaaaga
     1561 acggcggact gtctttgaca gtcgaaaggg ttagctctga taagggttta tctgtaacgc
     1621 tctttgccgg gaaaagcaaa atataaaaca gtcaaaaaaa gcgagttgtc aatttaatga
     1681 cagctcactt tttgcttttt atgtttacga atcagaaaaa ctatcaacag aaaaataagt
     1741 attccgacgg agctgccaca ggaatcaacg aacacgtcga aaattcggca ggctctttcc
     1801 ggcacaaaac actggtggat ttcatcgctt acggcgtaaa gaacgcaggg aataagcgga
     1861 atataaaact tgttttttga aaaagtaaaa caaattgctc cgctcatgca aaaaccgagt
     1921 gcggcatatt ccgtgaaatg ggcgattttt ctcagcacgt tgtgtgaaac gatgttgccg
     1981 agatactcga ttattatgct caaaacaccc tcgctgactt cctttgattg ctcgccgttt
     2041 tcgcttgaaa agctgaaaat gatatacatg acggcggcgg taagaagcca gaaaataatc
     2101 gaaccgatta tcttagacca cttcattaac tgtgacctcc cgagatcatg cacacgctct
     2161 caaaggcagg ggcgaagtag agtccgtcaa cacccttgtt gacaactgcg taggacgagc
     2221 cgccgagcat cggaagaaat acggctttat cgagaatata tttttccgca ttcacgcagc
     2281 cgttgagctt tgcggcaccc gaataggtcg taattatcga attgacggtt ttatcgtaat
     2341 tcttatcggc aaagccgaag atatttgacg agctgtcaga cgtgaatttc ttcaaggtat
     2401 ccgtaacggt tgaggagtcg gtcgtgattt tgtgataaag cacctgatag cttgtgttat
     2461 aaatcattgt gcttatttgc tcggctgttt taacctcaac cttggcaaga attgaggttg
     2521 agaaaactct ctgccagttt tgaatcgttt gctgcataag aggtgcattc tcctcggcgc
     2581 aggttatgac gacctcggct gtggttatat cgagcttttt gagtcctttt ttccaaaggg
     2641 tcagtgcctg ctgctcattg taggctattc cgcttacatt gcctgccgct tcacggtagg
     2701 agttttcgcc gtagcgaacg cagtccggga cgataccctt ggtgaaagcg ttatcggaat
     2761 tcgccgtcag ctcacttgtt tttgtaacca ttgcaagcgc acggcgcata tcctcgtcgg
     2821 agagaacgga atcagagcag ttgaagcaaa ggccgtatat catgttttcg gttgagtaat
     2881 ttgacagctt gtcgttgtcg ctcagttcat tctttgccgc ttcggacaga aacgcacagt
     2941 cgtatgtgcg ctgtctgatt ttcttgacaa ccgacgcttc ctccgtgttg acgtagaagt
     3001 aaagctcatc ggggttgact tttacgtttc ccttgtattc gtcattcttg tacatggaaa
     3061 gggtattgtc ctcagcccat cttgaaagat agaaaggtcc gttgcagaat gtatatttga
     3121 ggtcaaggca atattttgca tgagttgcct tgaaaaattc ctcgtggcag ggcattgcga
     3181 cgggaagtgt gagaatccgc aaaaagtcgg gatcctcacg ttcaagggta atgacaaggg
     3241 tcgaatcgtc ctgagccgat atttcgagcg cagatgtcgg ctgcttgccg ctgttgacgg
     3301 cggaagcgtt cttgatgctg aacagctttt cggcatcgcc tgcttgggtt gcaggatcaa
     3361 gcgcacggct tattccgtaa acaaagtcgt cggcaattac ctgagttcgg tatgtatcct
     3421 tataaccctc gggaagtacg tcctcaaatg aatttagaag cttccagtgg gtatcctttc
     3481 gaagcttgaa agtgtaggtc aaaccgtcct ttgagatctc ccagctttcg gcaacgccgg
     3541 gaattatttt ataatcctta tcgagcctga cgagaccttc aaagcagttc gctatcatca
     3601 gcttagcttc cttggagtcc gcaacctgcg gatcaaggca aatcgggtcc gactcaatgg
     3661 ggagtatgag aatgtcttcg ctgctgccgc cgcaggcgca aaggcttatc gctgcgacaa
     3721 gagcaagaaa aagtgacagt attttttttg gcattataaa gacctccgaa aagggaattg
     3781 aatttcagtc aattatgata nnnnnnnnnn taacattata gcaaatacat acaaaaaaag
     3841 ccagttattt ttgaaaagaa aatgaattgt aaatactttg attttttggt ataattatat
     3901 aatcataaac tatatcacgg agtgataata tgtcagacgg tttaatatac ataatttgca
     3961 ttttgctgat tgccgtaatc ggcataatga tatacacttt atctcagata aaaaaactga
     4021 aaaataatga cgagacgtcg caaattgata aacagctcgg tgcagttctc atgcttatga
     4081 agcagaattc cggaagcaat gcggaggaat ttgaacgcaa cagacgcgag acggcaaatt
     4141 cattgaatca gatgaccgaa aagctcgaca caatgacacg tcagaactat gaaacgctta
     4201 ttaagctcaa caaggacatg acggagtcgc tcaaccttat aagaaccaac agcatggagc
     4261 agaatgagaa acagacgcga gtggttgacg ctgccatagc gaagatgcag gagagcaatg
     4321 aaaagaagct cgaccagatg agggcgactg ttgacgaaaa gcttacatca acgcttgcaa
     4381 caaggctcga ttcatcgttc aagacagttt ccgaacagct tgaaaatgtt tataaatctc
     4441 tcggtgagat gaaggagctt tcaaccggag ttactacaaa cgttactgca ctcaacaggg
     4501 tgcttacaaa cgttaaaacg aggggaacat gggctgaaat tcagcttgag ggcatactcg
     4561 atcagac
//
LOCUS       DAWBSW010000163         4532 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3338_length_4532_cov_2.175787, whole genome shotgun sequence.
ACCESSION   DAWBSW010000163 DAWBSW010000000
VERSION     DAWBSW010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_4532_cov_2.175787"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1309..1318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1295 a          987 c          872 g         1368 t
ORIGIN      
        1 cgtcaacaac ggcaagtccg attcccgaac cgtgaacagt catatttgtc ttgtaaaatt
       61 tctctttaac atgaggcaga tcctcagaag atattccgca gcccgtgtcg gaaattacaa
      121 tcgtaatttt atcgtccttg atctccgttg aaacaacaat ttttcctccc tgttttgtat
      181 atttcaaagc gttgtcaaga atgttgacaa aaacctgctt tattctgtcg ggatctccgc
      241 tcatcggagc cggcagattc ggggcattat atataagctc aatgccctca cgcattgctc
      301 tgtccttgaa agcaaataca gtttcgtcaa gttcggcaag tatgtccatt ttttcgtttt
      361 tgagaaccat tctgccgctc tgcattcttg aaaaatcaag aagctcctca acaattccgt
      421 tcaatcttga agattcgctg attattacgt tcatgcctcg tttggtaaga tttgggtcgg
      481 tatcgccaat ctgtaaaagc gtttcgcccc agccctttat ggctgtcagc ggtgttctca
      541 attcatgtga aacggttgaa atgaaatcat tttttatttt atccgaagtt tttatcttct
      601 ctgccatatc attgatagtg ttgcagagct ggccgacctc atctttatat aaataatggt
      661 ctattcgagc gtcataattg ccgtcggcga tcattttagc ggtgtcgcaa acctcctgaa
      721 tcggccgtac aatcgaactg ataaaaacag ttccggagat tacaagaagc aaaacaagaa
      781 gaagaaaaac aagcgaaatt atgaaatacc aggttttgat ttgattgtcg atctcgtcaa
      841 gagaaatcat atacctgacc gaaccgacct gtttatcctc cgcatcggtg ataatttttg
      901 taagggcgat tatcttttcg cccgatgaat tttcaccgtg ccataatgca actttattgc
      961 tcgcggtcat tgcgtcctca tactcgggaa tttttgtctt actgtttacg gggaaaccgc
     1021 tcgaagagat aaaaacactg ccgtacttat cgagcaccca aacctcaaca gatgatttat
     1081 cgttaaaatc gcttatatat tcacgggcac ccttttcaaa aacagcatca tcggttgtgg
     1141 cataattctc gaaataaacg tccacaattc cgtttgcgta cgattcgatt tttctttcaa
     1201 ctgtcgcata gtaataagag tgaataaaat atgcggcggc aacacagatg ataagcaaaa
     1261 cagcaacaat tatggtcgag gattgaaata accaacgctt tgtcaatcnn nnnnnnnnga
     1321 aattgacctt gccgtttctt tttttcatct gtatcacctc ttttcaaaaa ttatgtttaa
     1381 gccttaaatc cagacatagc cgaagcccca gcgtgtaagc aaatgcttgg gattagacgg
     1441 atccggctca attttctttc tgattcgtct gatatttaca tcaacgattt tttcgtctcc
     1501 gaaatattcc gttccccaaa catgattgag aatatccgtt ctgctgagat ttgttccctg
     1561 gttacggaag aaatattcaa gaatctgaaa ttcaacctgt gtaacgtcga tttttctgcc
     1621 gtcgcaggta agggttcggc ttttgcaatc gagcttaaat tcgcccgagg aaatgacgtt
     1681 ttcatcatcc ggctcctgtt tattcttcga tgaagtcgaa acacgtcttg caagtgcatc
     1741 gactctcgcc gtaaattcgg acggtgaaaa cggttttgta acataatcgt cggcgccgag
     1801 aagcaatccg ttaaccttgt ccatttcctg agtcttggct gtgagcatca ttataccgat
     1861 attgttgctc aaggctctga gttttttgca aagctcaagt ccgtccattc tcggcatcat
     1921 aatatcaagc acggcgatgg aaatctcatc gggttcaagc ttgtattttt cataagcgtc
     1981 aagtccgtca aaggcttcga caacatcata gcctgccctt ttaaggttga taacgataaa
     2041 ttctcttatc gtttcctcgt cctcggcaac aaggatcttt ataggtttca ttttcttcct
     2101 cctaaaactg ttgtgttaaa attatcctta aggctctcat ctttaactcc gaaatcggtg
     2161 ccgtcgtctg ttattgtata aacatattcc gtgctgccgt aggagctaag aagctttgag
     2221 cttttatatt ttgttttagc ctgctcatca ttcttgtcat aataataaat actgaacaga
     2281 agctttccga gcttatcctc ggagctgtcc caatggtaga aatcccactg tccgtcatta
     2341 ccgagaactg ttattcttcc tacccaactg ctcggaatat tgagaatata tccaccgtac
     2401 tcatcaataa ccgaatactg aaccgctctg agtttattgt tacggaattt aacccatttt
     2461 gtgtaataaa gattttttgt cggcactgcg gccttttcat ttaaagaggc gggaagagcg
     2521 gtgtttgagc tgttgtcaac aatagatgat cccggcatat ccacactgac gggaatctct
     2581 aaaaatccgt ccgagtcaat gtcctgtgct gaaagcctaa tatgacgaag cgtctgcgac
     2641 gtcatctgag tcgcaacatc aaacagagga gcgacaagcg aattggtttc atcgtcccaa
     2701 taaataacct cggttatcat gtcgttatcg cctttgaaag cttcaacata aataaggcgc
     2761 aaatcctcaa ccttttcaac ctttatattt gaatatgatg aaatatttcc gtcggtctta
     2821 accgagctga gcgtctgaag cgacgaagat tttgcatcaa aagtatatgc cgtcgcagat
     2881 gaagtataat gatcggtaac cgttgaatca acattcattg tgaaaatatc gtcgaagccg
     2941 tcgccgttga catcaataac gtcaaggtac gaataaggga aggatgaaat aagcttcaaa
     3001 tcgcctgcgg atatatcgta aaccgcaaag gctttgtttg tcttgctcga aaaaagattc
     3061 cagcctatta taatttccga ttttccgtca tggttcaaat cggcaagctt aacctcatca
     3121 acggagctgc cgagaccgtc ataggaattg acgggaacga aagcgttgtt tttatactca
     3181 aaataataaa tctttacaag atccggattt ttcttctcgg tataaaaaac aatggcttct
     3241 tcgtccttgt ccatatcaaa atcatttaca ataaacgcag agcgatagag tccgttttca
     3301 ggggtggaaa ggctgtaatc tttatcgaca agcttttcaa acgaatcctg caagctctga
     3361 tattcacccg aaagcttcgg cggacgaagc aaattatcaa aggaggaaaa tataaaagaa
     3421 tcgcaataat acatgaaaaa gccaaaaagc atttcaatcg cttcatcaga atcactcctt
     3481 tcttaattat caaaaccgtt taaaatatac ccattctaac agtcattaca ttataacaca
     3541 ccgtgtgtca cttttacaac ataaaaagta aatttttctt aaataaattg aataaaaaaa
     3601 ccgaccgaac aacacaaagg tcattcagtc ggtcaaatat tcagttttgt acaattacag
     3661 ccgaataaga tccgctcacc caacaatttt ttgaattttt gagagtcacg gtaaccggta
     3721 cagtgttctc cccttcttca agctttatat tggtgagatt gatatttact tcaagcatat
     3781 ttgcattgac gcttgcaagt gccgtctgcg ttccgacaac ggtaaccgaa ccgatagagc
     3841 tgaggtcgac atttccggca ctgacgcccg agccgccggt aatcataatt gaatttatat
     3901 tcggttcaag cttttttgtt gagagtccgt cggtattgac ctcaacgctg aacgatgtcg
     3961 ttccgtcgag gaatttagcg gttttaacat tcgatgcaag gaacgtaaag ctgttgtttg
     4021 aaggcgtaat attggcaaaa tcaattgtgc ctatctcaag cgaattggta gtgtcggaac
     4081 cgttctgtaa aacagcaacc ctgaccctgc tcggagaaat actgtaaagc aaaggactgt
     4141 tgatataatc agaaggtgtg ttcttgaacg aaacggaaac ggctgatgtc tgcattttat
     4201 aaacaggaag cgttacaggt acagttaagc ggctttcgcc gttgatttta acatatttgg
     4261 aatcaatatc actgccgttg ctgagaacaa cagaagcatc aattgtttcg ctttgtgtaa
     4321 gcttcttttc aatgggaata tcggcataag cggcggtaat tgaattaacc tccgtctgtg
     4381 ctcccgaaac cttaacggtt tttgtctcat aaattatatc cgccttatcg aacatataat
     4441 catcggcggt gtagtcatca agctccgaaa taattctcgg agttacctca acttccttct
     4501 cggcatattt atcgaagtaa acctcaatat ag
//
LOCUS       DAWBSW010000164         4511 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3351_length_4511_cov_2.398564, whole genome shotgun sequence.
ACCESSION   DAWBSW010000164 DAWBSW010000000
VERSION     DAWBSW010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_4511_cov_2.398564"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1165 a          980 c          941 g         1425 t
ORIGIN      
        1 ccgttatatg tcggtgtggt gttgtattcc cacttgctct cctgaagaac atcatctccg
       61 ttcttgaagg ttacagtgta ggacttgatt gaccattgag cggtgaattt aaccgtcttt
      121 gtgtaatccc aattgaactg agcatctgtg ttatatgtag caccgtcggc atcacatacc
      181 cagcccgtga aggtgtatcc ctctcttgtc ggctctgcaa ttgttgttcc gtcaaccgaa
      241 ccgacggaat acttcgactg ataatatgca gtagtgtttg taactgtcga agaaccgtta
      301 aagctgccgc cgttcgggtc atagcttgct gtatatgaat tcggcatata gacgaatttc
      361 cattgaagat taccgccgcc ggcacacttt acaaaatagt ttttatcggc tttcacggtt
      421 cctgttgaaa ttactcctgt ttcatcggaa acagtatagc cgctcagggt atagcccgta
      481 atatctttat acggaacaga aacatcttcg ccgcctgtga agctcttaac gttcgaactt
      541 gtgccgatga tcggagattc ggaatttccg ttatagtccg ctccgcctgc cgcatcacga
      601 atatacgagg ttgtaaccgt gccggacact ctttcaagtt tacttctgtc ggtgtttcct
      661 gttgtaacag agcttgccgg attttcaaga tgaattgcca ttgcaagaat tgagttctga
      721 tatacgtcat atcccgcacc aaaccaattc tcctgcaagg cattggaaac agcaacatta
      781 acctttgctc tcaaaagagc cttgttgaca accgttactt caagaggaac gattccggcg
      841 cagtttacgt taccgttgtt gtcttcaata atggaatctc tttttgtgcc gaaacgcgcc
      901 gcacctttga cccgtacggt ctgtgatgag gttacttctt tattccagac gtcgttatag
      961 acaagtctcg gtctgcaata ttctctgtca accttttcgg tcgtagtttc atatttcgta
     1021 tcgcttacaa tatacgttcc tctgttttcg gtgtaagctt ttagatcctt tccgttattc
     1081 tcggacacag gctcataaga aatatttgaa acatcggaga aatagtaccc gaagcgtctt
     1141 tccttacaag agttgcttcc ttcccaggag tatgaaatta aataaccgat tttaagattc
     1201 ggaacatatt tcaggttgct gaaccttgtg gaatcaagaa caagtgatcc tttcggcgca
     1261 acagcctgag cctgagcgtt aacctcgccg ttgctgtaat ctgtaaggaa tgaggttccg
     1321 gaggtgccgc tgaaataaac gttgcccgtt actttggttc cgccgttatt tgttgcgttt
     1381 gtcggagtgc tgataacacc cttcaacgga tcaaagccgg ttgtgttgtt ggttctgttg
     1441 ccgtcacccg agcttcctgt aatgcccgag atccatgcaa tctgatgaag ctcatggttg
     1501 atgcctctgt ttgtatatgc tcttgaagcc gccgctacgg gcttatcata cggagcatag
     1561 cataatgaat atgcgtaagc ttcaagagta aaaccgtcaa cgacataatg agctgtccat
     1621 ttcaggaatc tgtatccgcc ggcgggaaca gccgaattca tcttaccgcc cgtacactga
     1681 atgttaacgg ggaatgtcga ataagtattg cctgttgttg aagcggccgc acccgaattg
     1741 gtcgttccac cgtatgtaac cgagctgagg ttgccgctcg ctgttgattt ccaatcggtc
     1801 gcgttatttg acgaggcttc aacggtaatc gttacgcttg taggcgtaca gtttgttacg
     1861 tcaaaataaa ccattgcacc ttttgcggcg tctgccgttg aagtattttt taccggtttt
     1921 ccgcttgtat caacgccgta gaaataccgg aatgactgat tgtcgttagg atcgagataa
     1981 actgtttccg gaacatagaa ggtaaccttc ggggtaccgg aatcatctat attttccgcc
     2041 agttcatcca tagcctcttt tatggctttt tcggcagcgt caattttcgt ctgatcataa
     2101 acagagctgt aagcgtcggt gccggctctg aaattatcat aggcatttgt gatttgctca
     2161 gctgcggcaa gagctgtttt cagtgaagcc tttgattctt cgattaaaga ggtgtttgca
     2221 atcgcagtcg cagccgtttc catagcagaa cgaagctttg tagtgtcgat ctgcggaaga
     2281 cgctttaaat gagtataaat ataatcgccc ttagcgccct tattggcatc ggctataaca
     2341 ttaaaattag cactttcaag aaattttaca gtttcatatc cggagatatc tgtgtttgta
     2401 tctattttaa tttcaatgat gggatctcct gccaagacat ttttcgttgc aaaataataa
     2461 agataatctc cgccggcttt catgttgagg tcaactacgc cgtcaccgag cttacctgcc
     2521 tccgagatcg gagtttttcc gacagccgta tatgtagctt tgttttcggg agcagtataa
     2581 ttattttgat tgttggcatt tctgattctg agatctttaa ttgaatttgt cggatcggta
     2641 tccgaacgat atccgagaaa aatataattt ccaccggctc cgtcgttaag atccttattt
     2701 ataactgtgt aattcacgcc cgtaataaca gctttagcct tatcggcttt tttcgagctg
     2761 tagccgagaa ccaaattagc aataaacttt gtacccggag catagtaata ctccttctca
     2821 tgcttctttg tatacgaagc ggcagaagcc tgtgttccga ataaattcaa atttaatacc
     2881 ggtgcacaag acagaagaat agcagcgcat aaaatcgcgc tgataatact ttttgccttt
     2941 ttcatcttaa tcaactccta taataatcca ttagaataca ttatactact tttaataatt
     3001 atttcaatag acattatgtt gaaatatagc gaataaaatt ttataaattg ttcaaaagga
     3061 cttgattttc ttgtaacatt actgtataat aaacagcgtt atgtgccggt gtgatggaat
     3121 tggcagacgt gccggactca aaatccggtg gtagcgatac cgtaccggtt cgaccccggt
     3181 caccggcacc atacataata atgtaacaat aaagaaaagc cacacttttg tgtggttttt
     3241 tctatttaga ttatagaatt ttgtgttaga agctccatga gatttttctg caaagctttt
     3301 acatcgccga gcttaggtgc aactttttca aaaaagttaa ttattatttt cagatataac
     3361 agtgtagcta aaaagagggc ctgtgcgatt gaacacagac ccatattttt ttactttaag
     3421 tcggcacctg tactgcccgt tgcaatcaaa aggacgatgc gtgcgtcgtt tgcatttata
     3481 atgccgcttc tgtcaagatc gccggctttg agctggttgg cattgaaatt aacgatgcct
     3541 accgaatagc ggaggatttc aagtgcatcc gaggaattga cctttccgtc ggaattaacg
     3601 tctccgagct tatatgaagg agccggagtc ggttttactt cattaggcat tttataattc
     3661 ttgaattcag gccagtaggt attctgcgcc ttatatcccc tgctctccga ctttgcagcc
     3721 ttgttagccg gtctctcgaa atagtaacac cagtcatagc ctgcggtata tgctccgctt
     3781 gcggtgtttg aaacattctt gagtttatca agaatatagc ctgtgtcgct cttattgtag
     3841 gtaagctcat atttaaggaa ataaagctga ccgttaagcg ttttccagtt gtatccgttc
     3901 ttgttgcaga agcttataag attatcaaat cttgaaaggt tccactgaca tatgccgtag
     3961 ctgtcaccgc cgtcaccgta aaggttcggg ttaaagtttg actccgcctc gatatttgca
     4021 agaacgccgc atgcgcctgc gttattgagc ttaagctcct ttatgcaaaa ttcataaatg
     4081 gtttttgcgt ttgccgaagc tgccgaagca gaaaacggga tacagcatac aaccataaca
     4141 acgctgagaa aagcaagtgt aatcctcttt atcaaagtgg tttttttcat aaaatacctt
     4201 ccttacagaa tttttcaaca cttattctta taccacgaaa atgttgtgta aatatgtctt
     4261 ttttgttaat gttgtaacct tttgtaacta attgagataa ttatttacct gtatgttctt
     4321 attaggaatt ttactccgtg ctaaaatgat ttaatcttag ctctttccga ggagggttta
     4381 tgctcaatgc ttttcttttc gcacatctgt tgaatatcac tttttcagcc aatagccctg
     4441 tgcgatgcaa accgacacag taaaagcccg attttcgaca aaaatcatgc ctcccctgca
     4501 gcaaacaaga g
//
LOCUS       DAWBSW010000165         4454 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3401_length_4454_cov_3.041146, whole genome shotgun sequence.
ACCESSION   DAWBSW010000165 DAWBSW010000000
VERSION     DAWBSW010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_4454_cov_3.041146"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1174..1183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1225 a          901 c          998 g         1320 t
ORIGIN      
        1 ttacaattcc caatccttta ctttctcatt ctttgttcag ttttgagggt gtgaatcctg
       61 aaagatcgct tttagcggtc ttttttttgt tgtcttaata tttttgttta tgctttgcaa
      121 aaattaaaaa ttgtttagtc attataaata agcagtcttt taccgataaa tagattagtt
      181 tttattgttt tttttattct gttgtgttag aataggtttt agattttgtg tagtttttaa
      241 ctttaaagtt agaagatact tctccgcgct cccgtatggg atttgtcttg gcttttcgag
      301 actgagaagc tttgtaaaag cttttatatg ctcagcttgt gataatggaa acgtgcattt
      361 aacgactgcc gaagaaggag cacaaaaaat gaaaaaagtt tgggttatag ttatatccgt
      421 tttactattc ttttcggtcg caatagttgc gataaattat ctctaccctt attatgtgca
      481 tttgagcagc gtgaaatatg ccaacgaagt aaaggaaagc aaaatcgaca aggacaattt
      541 tattttatgc tcaaaaattt ctgaggacaa gaccgttaat tcaatgtata taacttttta
      601 tgttctcgat aaagcttctc agacaattcg ttttgaatgt cccgacggtt ggcgtttaca
      661 ggatttcaag tatcttggat ttgaaaatga tacggataat attcttgcaa tatcgggcga
      721 tatcggaaca tacagatatg tttacgacgg gcaaacatgg aaagaacaaa ccgattatga
      781 taaggaatat gttcctgata ctaagcttta tttgataaat aatcttaatg atgaataaaa
      841 ccggttgagc atgggatctt tctacggggt atctgacggt gacaaaaaag caacaaaact
      901 aagtgaataa attcaaggcg atatataatc gcaccaacta taataattta ataaacagaa
      961 aacatccaaa tacaacgggg gtatgaaatt ggcaggtaaa atcaacatga agagtttttg
     1021 gcatcagttg cggccgatgc tcggatacaa tgccgcgaat attcttttcg gcggatctac
     1081 ctacataatc agcctcttct ttatgtcata tctgacagag gttgagggcc ttaacaccaa
     1141 gcaggcgggc tgggtatttg cttttgctca catnnnnnnn nnncttattc ccgatcgaac
     1201 aaaatcgaag ttcggcaagc atcgccgcta tcttatttgg ggcattgcac ctatttttat
     1261 ttcatacttc cttttgtgga actcgttcgg tctttcacaa atcgtcagca cgaatgtcat
     1321 cactgtttat tacacctgtg cgtatatgct ctttaacact gcatacacgc tcgtttgtgt
     1381 gccttataca gcaatgcttc ccgaaatcgc accggaatac tcgctgagaa cgcagtacaa
     1441 atccgtagag tatatcttta attcggtagg aatggtatct tcatttgtgc tcgtttccgc
     1501 cgttctcgga tttacagatg ttaaaactct tgaagcgacg ccggaaatca gaagcaagtt
     1561 cctcttcatg ggtcttgtgc tttcactgtt cttctctctt ccgcttctta tcacgttttt
     1621 ctcgtccaaa gagaaggatt caagagacat tgttgttccg cccgttaatt tcccgaatat
     1681 gatcaacgaa tacaagcttg ttttcaagaa caagtcattc agacagtatt ttgcactcag
     1741 cacgttttat tcgatttcaa agggcttcta caataactct aaccagtatt ttataaagca
     1801 tgccgcaaag cgctggaaca agtataacat tctcaatacc gttgcaggtg ttgccgaggc
     1861 ttcgggattc ccgataaact atgcgctgac aatgaaatac ggcaaacagc tctgcggcaa
     1921 gctgctcgga ccgattatga tagtcggcct tgccatttcg ctttttgtca atgagagcac
     1981 gccgacatgg gtcctttatc tcgcatccgt tctttattgc ttcggattct cgggcgtggg
     2041 ctttgtcgga acaaacactc agcccgacgt taccgacgtt gacgagctta tcacaggtcg
     2101 ccgccgtgag ggagttattt ccacgttctc atcgcttatc aaaaagacca tcaacggctt
     2161 gatggcggcg cttacaggtt atattctcgc cgcattcggc ttccataccg gcgagggtgc
     2221 ggttcagact actatgggta tgttcggaat caagtttaca tattccattt tgccgattat
     2281 tttcgctctg ctttcaatta tcggaattta ccgttacaag atgacaaaga gcgatcacga
     2341 acttataaag aaggctattg ccgagaagaa agaaacgggc aaggctgttc tttcggctga
     2401 ggaaagaaaa atttgtgagg atattgcagg tcaggaatgg ggtaacatgt ggataggcaa
     2461 agctgaaatt ccgcaggagc ttgcagagct gaccgaagta aatacaaata actgacggag
     2521 gtatttttat gacacgttat gaatatgcga aggcgaaata tgccgagata ggcattgaca
     2581 cggaaaaggt tatcgaaatg cttgcaacgg ttcctgtttc cgttcactgc tggcagggcg
     2641 atgacgttac gggctttgac tcaaagcaag cactctccgg cggaattcag acaacgggaa
     2701 actatccggg aaaggcaaca actccggaag agctcatgac tgacctcgat aaggcttttt
     2761 cacttgttcc gggcaagaaa aagctcaatc ttcatgcgag ctatgctata tttgaggacg
     2821 gcgaatttgc cgacagagat aagattgagc cgaagcactt cgcgaaatgg gtgaagttcg
     2881 caaaggacag gggaatgggc attgatttta acccgacctt cttttctcat ccgatggtta
     2941 aggataatct tacactttct tcacccgatg aaaaggttcg tacattttgg gtaaatcacg
     3001 gtaaggcttg ccttaggatt gccgagtatt ttgcaaacga aacgggtgtt ccttgcgtaa
     3061 tgaacatatg gattccggac ggatacaagg atattcctgc cgacagactt gctccgagag
     3121 cgagattcaa aaaatcgctt gatgaaattc tttcaatacc atatgacaag agcaaggttt
     3181 atattacgct cgaatccaag gttttcggca tcggtcttga atcctatacg gtcggttctg
     3241 ccgaatttgc actctcatat gtaaactaca agggtatcac tccgctcatg gacaacggtc
     3301 attatcaccc gacagaggtc gtttccgata agatttcctc gttgcttctt ttcaatgaga
     3361 agatcgcact ccatataaca cgccctgtcc gctgggacag cgaccatgtt gttctttttg
     3421 acgacgagac aaaggaaatc gcaaaggaaa tcgtcgcgca tgatgcgctt gaccgtgtat
     3481 tcattgcaac ggactatttt gacgcttcga tcaaccgtat tgcagcatgg acaatcggtc
     3541 tgagaaatgt tcagaaggca cttctcaatg cgcttctcac tccgcataag gagcttgcaa
     3601 agcttcagga cgagagtaaa ttcacaaagc tgctcgcaac tcaggaggag ctgaagttct
     3661 tcccgtccgg cgatgtttgg gcggaatact gtgcacgtca gggtgtgatc gccgacggtg
     3721 catggctaaa cgaagtcgaa aaatatgaat gtgaagtcct ttcaaagaga taatttttga
     3781 tgtatatttg ttatatcgct gctttgattc tatcggaaaa aattgtatat tgacgtttgt
     3841 tttgtaagaa acggacaaag actgaaaaaa atcgggaaaa agcggtggat tttgtatttt
     3901 tattattgac aaatcgcgtt ttacactata taatgtttgg tgcatgacat ttgaggtgaa
     3961 gagtatttat gtatttcgtt gagcttgagc ctgtctttaa taatgagggc atggcgctgc
     4021 ctttgaattt cacgattgac atgaatgatt gtgatattta cggcggaaaa tcttttaatt
     4081 cacccgttcg cgttatggga gaaatcaaga actcgacggg catagtcagc attaaagcga
     4141 aggctgattt tactctcaat ctcacctgcg acagatgtgc ggccgatttt gaccgtacct
     4201 tttcaatgcc gatcgagcat gtgcttgtca cggagcttaa caatgacgag ggcaatgacg
     4261 aattcattgt tgtcgattcg ctccgatacg atgccgaacc gcttgttcgg gaggatattc
     4321 tcctgaacct gcctaccaag gtgttgtgca gggatgactg cctcgggatc tgtccccggt
     4381 gcggcaaaga cttaaatgac ggtccgtgtg actgcggcaa ggagtacgac cctcgctggg
     4441 acgccctctt acag
//
LOCUS       DAWBSW010000166         4454 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3400_length_4454_cov_3.105251, whole genome shotgun sequence.
ACCESSION   DAWBSW010000166 DAWBSW010000000
VERSION     DAWBSW010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_4454_cov_3.105251"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1293 a          954 c         1067 g         1140 t
ORIGIN      
        1 tgtgtaaaat gaacgaggaa atgacaggct ttgttccgtt tgatgacggc gaaaccttca
       61 aagaaattac gccgcacgat tatgttgagg gtccttgcat gatgaaaaag gacggcaaat
      121 attatttcat gtattcctgc ggaagctggg gagagggaac ataccgtgtt gactattcaa
      181 tttccgacag ccctctcggc ccgtttgaga gcctcggaac aatcctttcc tcgcagcccg
      241 gagttgccga aggacccgga cacaacggtt attttctgct tgagggcggc gagatgctta
      301 tggtttacca ccgccgccgt gagaatatca aagaccgtga cgcaagattt ctttgcattg
      361 ataaggttga atttgacggc gataaaatca tgccgataaa aatgacctga ggagcaaatt
      421 tcatggaacc gagaaagcta attgaaacgc ttgccgttgc cgaacggctc aaggacgcaa
      481 caagacattg ctatacctcg ggcggacgca gggaaagcgt tgccgaacat tcgtggcgaa
      541 tgactcttat ggcctatttt gtcagtgatg aatttcccaa agcggatttt gaaaaaatat
      601 ttaaaatgtg cctcatacat gaccttggcg aagcattcac cggagatatc cccacattcg
      661 agaaaaccgt agaaaatgaa aagcaggaag aaatgcttct ttttgattgg gtaaaatctc
      721 tgaccgaacc gttttgcacg gaactggcgg cgctttacag tgaaatggaa gaaagaaaaa
      781 ctcttgaagc gaaaatttac aaggctatag acagcctgga agctcttatt cagcataatg
      841 agagtgacat ttcaacatgg cttccgcttg aatatgacct gcaaatgaca tacggaaacg
      901 acaaaacgga cttttcggaa tatatgaagt ccttgcgtga gcttgtacga aacgacagcg
      961 ttgagaaaat aaaacaaaaa gaatcatgaa tgaaaaaaca gcaatgtatt gtgactgata
     1021 cattgctgct tttcttgtca gtttataatt ctttgctcca tttttgagcc ggggttgtcg
     1081 gcaggtcgag aaacgtaaac cggcatattt tctttatctt ctcatgagct tgctcgtgta
     1141 gagcttatgt atgccgaatt tccagcaaag gagcataccg aacactgcgc cgacagccgc
     1201 ctgaagaatc tggaacggaa gctttaatat tgccgcctcg ggagtgccgt agaaaaatgc
     1261 tctgccgatt gaatagccga caaccattat tactgcgccg acagcgacgc cgattcccga
     1321 gctgagcacg ggcttctttt tcaaaacata gtgggagaaa acggaaatga ctattgcttg
     1381 caagccgtgt gttataagtg aaacccacat cggggtcgga tagaagaaga gatctccgag
     1441 aaatgctccg actccgccga ccataaatgc tgccaacggg tcaagaatta ttgccgccgt
     1501 gcatataacg atgtcattaa gatagagatg tccgcccgct acgggaactc cgaaggagct
     1561 gagtgccacg ttcatagcca tgagcactgc cgttgtgcaa agccacattg tattctgcct
     1621 tgctgttctt tttgtttgca ttatgttttc ctccttgcaa atctgttgga aatattatat
     1681 aatagctctg ataatataaa atagtcagaa acggagaaaa atgtataacc agatgaaata
     1741 tataatcgac aaacaaaaac accccgtata tctgcaaatt tacaggcaga ttcgagacga
     1801 tataacaagc ggagcatttg aatttaacag taagctgccg tcaaagaggc tgcttgcaga
     1861 tgagctcgga gtaagcacga taaccgttga acatgcgtat gagcttctgt gcgacgaggg
     1921 atatatcgag ccgagagagc gaagcggata ttttgttatt ttcagctcca atgacggctt
     1981 tgccaaggtc tcgcatatat cagattcgct gaaattccat gcgtcttcga cacactcaag
     2041 cttttcaatt tcacttttaa gcaagacaat gcgcagggtc ttgactgatt ataatgaagt
     2101 tattcttgaa aagtcaccga atgtcggctg cgtagagctt agagccgcat tgaagcagta
     2161 ccttgcacgc aacaggggaa taaacgttga aattgagcaa atagttatcg gttcgggtgc
     2221 ggaatatctt tacaggctta tcgtagacct gctcggtcgt gataaggtct atgccattga
     2281 atcgccgtct tatcaaaaaa ttgagcaggt ttaccgtgcg gcgggtgtaa gatatgagat
     2341 gttgcctctt tgcaacgacg gaattgacgg agccgcactc gctaaaacag atgcggacgt
     2401 cctgcacaca acgccgtaca ggagcttccc gacaggcgtc acggcttcgg cttcaaaacg
     2461 ctatgaatac atacgctggt cggaaaaagg cgagagattt atcatcgaat cggactacga
     2521 ttccgagttt tcggtttcat caaagccgac agagacaatt ttttcccttt ccgatcacga
     2581 taacgttatt tatctcaata cgttttcaaa aacaatttct ccgtccatgc gtgtcggata
     2641 tatggttctt cccaaaaggc ttgtcgagcc gttcaatgac agactcggat tttattcttg
     2701 cactgttcct acttttgaac agcttgtcct caccgagctg ataaataacg gcgattttga
     2761 acgccatatc aatcgagagc gccgaaaaaa acgccgcgaa gctgagacaa agacagacag
     2821 aaatgattaa aaagcttgaa ttattcttgc aaatagatta tattgtttat acacaactat
     2881 cggaggtaac ttatgaaaga tttcagatta agacaggtac accttgattt tcacacttcg
     2941 ccgctcattc ccgatgtcgg cgcaagattt gataaaaagg aatggcagga gacgctgaag
     3001 atgggtcatg ttgattcaat tacggttttc tcaaagtgcc atcacggata ttcatatcat
     3061 ccgacaaaag tcaacacaat gaatccgaca ctgaaattcg accttctcaa ggctcagctt
     3121 gaagcctgcg aggaaatcgg agttcgtgct cccgtatata tctcggcagg cttcgatgaa
     3181 aaggacgcgg ttgagcatca cgattggctg agagtcgaac cgtcgaatat cggcaaggct
     3241 cccgattttg agcacgccgg ctatcatatg atgtgcttca atacggctta tcttgacaag
     3301 cttattgccg agatagagga ggttatggtc aactacaatc catgcgagat cttccttgat
     3361 attgtcggtg aaccgatgtg ctgctgcgaa aaatgccgtg cggacatgac cgcggcaggg
     3421 cttgattaca ataatattaa ggatgttgaa gccttcggca ggaaggtgta ccgcaactat
     3481 cttgacaggg tagaggaggc tgttcataag cataatcccg aaacaattat ttttcaaaat
     3541 tcgggtcatg tttcaaaggg caggcgtgat ctcattgaca cgatcgactg cctcgaattg
     3601 gagagccttc ctacgggcgg ctggggatac gaccatttcc cgatgtctgc ggcatatgcg
     3661 agaactcaac gcgattgctt cctcggcatg acgggtaaat ttcatcagac atggggagaa
     3721 ttcggcggat ttaagcatcc gaatgcactc cgttatgaga ctgcgctttc tcttgcggag
     3781 ggtggcggat gttcaatcgg cgaccagatg catccggaag gactgctcaa taaatcgacc
     3841 tatgccctta taggaaaggc ttatgcagag gtcgaagcta aagagccgtg gtgcaagggg
     3901 gtaaagtata ttgccgatat agctgttctt gctgcggaat gtatcaaagg cagaggcgaa
     3961 ggcggatggc acgatgcggg cgcaaaccgt attctacttg aaacaaacag actttataat
     4021 tttatagatc ttgatgaaga ttttacaaag tataaaatgc ttatcctgcc ggatatttgg
     4081 gtctttgacg aaaagattaa atcgaagata gacgactatc tttcgcaggg cggcaagctt
     4141 cttctttccg gcaaatcggg tcttgataaa aataacgagt tttgcattga tacaggagtt
     4201 aagtttatcg gcgagaacga attcagacca acatatttta tcccggaatt tgatacggta
     4261 aacggcaaaa ccgagtacct tatgcgtgcg gtgaattatc gctttgacaa tattgatgcc
     4321 gaaatcgttg caaacggaca gaatccctat tttaaccgca ccgccgagca tttctgctca
     4381 catcagcatg cgccgaacga cagaaggctt acctatccga ccgttgcgat caagggaaac
     4441 gtcgcttata tcgg
//
LOCUS       DAWBSW010000167         4449 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3412_length_4449_cov_2.325444, whole genome shotgun sequence.
ACCESSION   DAWBSW010000167 DAWBSW010000000
VERSION     DAWBSW010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3412_length_4449_cov_2.325444"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1533..1542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1320 a         1064 c         1002 g         1053 t
ORIGIN      
        1 ccgcaaagcg gtccgtactg aactgcaccg ataacgacca gaacaggtaa aaacgttatg
       61 gacgctctcg gcattttaag atagccgagc ggcgtaagac tcattacaat tatgagcgcg
      121 gtcaaaaccg ccacaaatat caatttctct tttctttctt cccttaaagc tactttcatt
      181 tttaaaaaac cttccgttta taaatatttt tcaaaccctc aagaataaga tcacggtcaa
      241 atataatctc gcgatcgcaa tttttaacaa tttcctccgc caagccgccg gtagcgaccg
      301 ttgttttaac tttccttccg aaatcccttt ccattcgcgc cgtaaggccg tcaagcatcg
      361 ctgccgttcc gaaaaccgta ccggactgca tacagtccac ggtgttcgta ccgattgatg
      421 tcggcggtgc agtcagcctg accgccggaa gctgcgaggt ctttttcgca agagcttcaa
      481 gcgaaatccc aacacctgcc gaaatcgtac agccaagaaa tactccgtcc tcatcaagga
      541 gaataatctt ggtagcagtt ccgagatcaa cgataaagca cggtaattca tacttatcta
      601 tcgccgcaac ggaggctaca gccaaatcgg caccgaggaa tccctctgta cccgtaacga
      661 ttttcaaccc cgttcctata tcagacgaca aaaccttcgg agtaacccct gtaacggtct
      721 caacagcact gcccacggca ttgacgacct cgggcacaac gctgcttatt attgcacccg
      781 aaaagccgga acagcttgca ttatgcagtt taaaaatatt aagaagctca acggaatact
      841 ggtcgcttgt tctctttctg tccgttgcca agcgagaagt aaacagcaat tcgtcgcctt
      901 cataaattcc catagttata ttggtattgc cgatatctat cgtcaaaagc atatcaatca
      961 ccatttagca ttatatttgt tttttattca aaagtcaata acgtcaatat aacagtcaca
     1021 ataaactgca tttttgggtt tttaagccaa ttataaatgc cgattttttg tatttttgcc
     1081 gaaaaattaa aggaatgttg aaaaaaatat aaaagtatga tacgataagc gaagtaaacg
     1141 gaggctcatt atgagaattt tacaaaaagg gtttaattat tcacaggacg gtcccgggaa
     1201 ccgtctcatc tatcacctgc aagggtgcaa tttcaaatgc aaatggtgct ccaatcccga
     1261 gagtatgtcg cttttttccg acaaggcgga ggatgttccg aacgacgatg ttgttaaaga
     1321 aatcgagcga agcagaatga tgttctttga cggcggcggg gtcaccttca ccggagggga
     1381 accgactctg cagcccgatg accttatata tgtgttaaaa aaggtacggg agcttggggt
     1441 aaatacggct atcgaaaaca acggttcaag cccgagactt tgcgaaattt ccgactacgt
     1501 tgattatatg atagtcgaca taaagcatcc cgnnnnnnnn nntatacata aaaagtatgt
     1561 tgaacacagc aacaaaatga cggttgaaaa tctcataaaa ctttcgcata agcgcaatca
     1621 gcttcacgtc agaatcccgc tgataaatca cgtcaacaca aatcccgagc cgttcgccga
     1681 gctgttcaaa aaaatggata tggcaaacac ggtcgtggaa atcctcgcct accatgaatt
     1741 cggcaaatgc aaatggacag aggagtatca aattacggac ggatttgtga ccgatgaaca
     1801 aataaaaata ttcaaaaatc gtcttgaaaa ggacggcata cattttatag aaacctgaac
     1861 ggaggtagcg gtatgatata tgacatctat acgcctgaaa aagtgaccga aaaggcaaag
     1921 gtgctcttca aacacgagag agccaaggac agacttgacg gctggttcat cgccaaggaa
     1981 aaggagctcc ggatctacga ttcgcttgcc gaatacgatg aggaggtcaa aaaggctctc
     2041 ataattaagg aaatcgtaaa ggatatccca attgatcttg atgaaaatca gatctttgca
     2101 ggcactcagg acgattcctt tgcacgaagc tatgcgctta tcaatccgtc gttcagggtt
     2161 gaggagtttg caggctactg cgaccctgcc gctgttttcg gcgatatcgt gccgaatgag
     2221 gaatttaccg aggagagaat taagaaagcc aaggacgaat ttctcaagac agaatatgca
     2281 aagaagctca aaaaggtata taccgaaagc gaaagctaca cgaaggaagt tatatatttc
     2341 tttgaacagg ttacgggaca tcttattcct gacttccgtt ttgctttgaa gcacgggctt
     2401 aaagctctca tcaatgagat gaagaacaag gagggcaacg gctacaaggc atttgttatc
     2461 gcccttgagg cggcgtgcga ccttgccgca agatacgaaa agctcgcaag agaaaaggca
     2521 gaagctctgc cggagccgca gaaatcgcag tttattctca tggcagatac gctcaaaaaa
     2581 gtccccttca acggagcgga gaacctctac gaagcaattc agtcgttcat catattatgg
     2641 cagatgatgt gccttgagca ggctccgaac ccgttcgcat tctccgttgg aaatgccgac
     2701 agaatttttg agccgtacag aaacggactc gaccgtgacg aaactgcggc cttattcaag
     2761 catttccttg ttttcttcaa tgttgcggac agaagctggg caatctcaca gaacatcatc
     2821 gtcagcggac gcagcgtctg cggcgaggat ctgacaaacc cgactacata tgcgctgctt
     2881 gacgcttact acgatatgaa cctgccgcag ccgatactct cggttaagct gcacaagaac
     2941 actcccgaaa agctttaccg tgagcttggc agatttttct tctcccccgg cgttctcact
     3001 ccatcgatgt tcaacgacga cgcactcttt gaaacgctcg gctcacacgg cgttgacaag
     3061 gacgatctcc cggatatcgc aatcgcaggc tgtcaggaac cgctgattat gggcgaggac
     3121 aacgccaaca cgaccaacag ttggttcaac ctgccgaagg ttcttgaaat gacccttacg
     3181 ggcggctatt cactcatcac gggcgaaaag ctttgcgatg cagaaaaagc accgcttgaa
     3241 aatatccgtg aggaattcta taaaaacgta aaattctttg cgcacgaaat ggctaaatcc
     3301 gccaacggcg catcggaggc tatttcaact cagcgtgttc cgtttctctc ctgctttatg
     3361 ggcggtcaac gcaacggaat cgacgcacga gacattcaca ggcagggcac gaagtataac
     3421 ggaagcggct gtcttatcca tggcacaacc gttgttgccg actgcttcgc agcaatcgac
     3481 aagcttctta aagagagacc gcaggacgca aataagctca ttcctgcgct caaggcagat
     3541 tttgtgggct acgaggattt gcgtgacttt ctgcttggtg cagataaatt cggaaacaac
     3601 attgaaaagg ttgacaacga ggcaggcgag atcgcatcaa gggttgccga tgtgattgcg
     3661 aacgagaaaa actatctcgg caatccgttc agacccgatt tcagcacccc gtcaacccat
     3721 cttctctacg gctactgggt cggcgcaaca cccgacggca gaaaaagccg tgatatgctc
     3781 aactacggcg ttgatccgct ctacggcagt gccgaaaacg gactgggctt ccgtgtgctt
     3841 tcaaatatga agatgccgtt tgacaagttt aacggcggct acgcctccca cctcggcgtt
     3901 gacccgaagt atttcaaggc ggaaacatat gaggacaagg gcgttgaatt cagggataaa
     3961 atcgtaaatc cgctgttttt caatccgctt aaagagggca tttctccgtt ctacctctat
     4021 gttaatgtaa caacagccga aattctcaga aaggttctcg ccgaacccga gaaatacgca
     4081 ccgagcggag tgtatattat gagaatccac gggacattcg taaacttcct cgacctctcc
     4141 cctgctatcc agcaggatat cattcgccgt ctcgatccca aatcggcgaa tttggagtga
     4201 gagaagagtc ttttgagaca taattaagct ccccttactt caaggggagg ctgatcaaaa
     4261 gaaagccgta caaaggagca cccaaatgaa ctcactcgga attgatatag gaacaacggg
     4321 aatatgcgga attatcgttg actgtgaaaa cggcgaaatt ctcgattccg taaatatgaa
     4381 caacgattcg ttcataaaaa ccgacaagga atatgagcga attcaatcgt ccgagcggat
     4441 aacggaaat
//
LOCUS       DAWBSW010000168         4428 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3439_length_4428_cov_2.080723, whole genome shotgun sequence.
ACCESSION   DAWBSW010000168 DAWBSW010000000
VERSION     DAWBSW010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_4428_cov_2.080723"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    458..467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1236 a          841 c          953 g         1388 t
ORIGIN      
        1 atagttatgt ttgcgcagac gatgttgatg ttcccgtaga caacgggttt gatgttgacg
       61 aaaaagacat agatgttgac gagatatttg atgatgttca gaaaaaaata ttgaatatat
      121 taaacgataa agaaaaagcg ctttttataa agctttatat agaaaagaaa gaagttaatt
      181 tgataaccaa agagatggat attacatatg ataatttccg aaaaagaaaa agtcggctca
      241 aaagaaaggt gacaaagtcg gttagatatc tgtatttctt tgcgcttgtg ttttctttca
      301 aaatttttca ctgagtttga caaagaaaca ggtgcttgcg gaggttaaaa atgaaagaaa
      361 gtatcaaatc tttggagaaa gaattaaaca gaataattga cgttgaactc gcaaaagatg
      421 tgtcacgtat tgatgaccga ctggttatgg cttgctgnnn nnnnnnntcg actcattatt
      481 ccgtaaaaaa caaaaacccg ttacaaagct gacaaagaaa ataaagatac ttctgattgc
      541 ggcaataata gcaatactcc ttgcgatagg ttcacttgga tattcgcagt ataagtacaa
      601 tatttttaat ttttccgatc attcaaccgt attgtttgac aattctcgta ataagagggt
      661 aggcgatttc actttaagtt atattcccga cgggttcacg ttgaattatg agagtgataa
      721 taaatatgaa cactcaaggg aatatgcaag cgaaagcgga ttctttttag ttactaaaca
      781 gtctaacata aacgaaattg atataaacac ggaatacaaa ggtgccaatg ttcaaacggt
      841 aggcggtata gactatatcg aattcggcga atccgaacac ggtcagggtg ttgtttggga
      901 agatgacgga tataagtata ttgtaagcgg caatgtttca agtcagcttt tgttgaaaat
      961 agcaacttcg gttagtaatg attaacaaaa acgcagcttc ttatttgtac aaatcggaga
     1021 agcgaaaaaa atctaagatt tttttgtcac aaagcggtaa attgttcata tatatatgga
     1081 tgtagtttat ggcaataaag atcggattcg gctctttatc tttaattgat tgaacctttt
     1141 aaatgcacgg cttgtcatcg aaggtctttg aagctgaaat atattttgtt gcttaatcac
     1201 agtataaatg ataactatgt cttatgttcc cacgcggaca tagtgtcaaa accgtacacg
     1261 gttacgtaaa tcgactttat ccgaccgcga atttcgcagc cgtgtgcggc ataaaaattg
     1321 tacgtattat cactttttga gagataatac gttctttttt taaaacagac tttaataatt
     1381 caatcttttt aattttcgtc aaaagttatt gaattttata taaggctgta ttataatatt
     1441 tctctataaa aatgtaagga gatatcataa ttggcaaaga ataaatttga aattgatatg
     1501 atcaacggta cgatcatgaa caaaatgatt tcgtttgcca ttccgctcat gctgtccgga
     1561 attttacagc tgttgttcaa cgccgttgat attatagttg tcggccgctt cagcggaagt
     1621 cagtcgcttg ccgcagtcgg ctcaacgtcg tcactcataa atatgttgac aaatcttttt
     1681 atcggaattt cgctcggcgc caatgtcctt gccgcacgct tctatgccgc cggtaagcat
     1741 aaagagatgt cggagaccgt gcatacttca attttgactg catttatcag cggagtttta
     1801 atgatttttg tcggcatttt cttttcgcgt ccggcacttg agcttatgga tacgccttcc
     1861 gatgttattg agcttgcgac gctttatatg cgcatttatt ttgtcggcat gccgtttttt
     1921 atgctttata actacggagc ggcgatactt cgtgcggtcg gcgataccaa aagaccgctt
     1981 atttttctca ttgtttcggg cgttgttaat gcctgtctca atcttctgct tgttattaaa
     2041 tttaatatgg acgttgcggg agttgcgatt gcaacggtta tttcgcagat gatttcctgc
     2101 gtgcttgtaa ttatatgcct ttacaaaacc gatgcggttt atcagcttcg atttaaaaag
     2161 ctgagaataa agtgggttta cctcggtcag attttcagaa taggtatccc tgccggaatt
     2221 caaagcacac ttatcagctt ttcaaacgtt cttttgcagt cctcggtcaa ttccttcggc
     2281 tcaattgcaa tggcaggcta cacggcggcg aacaacattc tcagctttct ttacatgggc
     2341 gcaaactcaa tcacacaggc gtgcatgagc ttcaccagtc agaattacgg agcgagaaag
     2401 ccgaagcgaa tggatagggt cctgcttgac ggcctgatac ttcaggttgt tatttgtctg
     2461 tcgctcggcg ttttggctta tgttttcggt aatcaggtat cgagcattta caccgacgat
     2521 gcgaacgtaa taaaatgctc tgtcgaaata ctggcgctga cgaccgtgcc gtattttctc
     2581 tgcggattta tggatacatt ccccggagtg ctcagaggta tgggacgttc aaccgtgccg
     2641 atgattcttt gcctgctcgg aacggtcgga gtgagaataa tatggatata ctgcttcttc
     2701 cctcataaca gaacacttgg ttatttattc atttcttacc ccgtttcatg gatagcaacg
     2761 atcctgatgc agcttgttta ttatattttt gtcagaaaaa cagtttacaa agagctgaaa
     2821 tcaacggctg aattcgttta acaataattt ttgcagcacc gtgatataat gctctgattt
     2881 cctcgatgct ccaccggcga gattaagtct gccgatacgt tattatttta ttaaatttgt
     2941 gtttttgaac gcttttattc attgactatt aaacgacgag cataggaaat ctaatataat
     3001 aacaaaaaaa ttccgaccct tgaaattcaa aggtcggata tttttattga attaaaacaa
     3061 tcaatatttg tatgtattct gctgagtcca tgcgccgtga acggttgccg acggcttaaa
     3121 accgagaacg tttgctgtac cgttgccgtt gagcggctgg tcaatcgagc tgcttacaag
     3181 cattccgttt gcgcctaccg ttgctttaag agttatgccg ggatagttga tctctgcctg
     3241 agtaaccttg atcggtccaa ggtcaacgct tgcaagatca agcggagctg cacactgttc
     3301 ggcatacttc ggcttcggat tctcaagagt gctcttttct gcgattgtaa cgattgttac
     3361 aaggtaattt gaaccgctct ttgtaatgct tgccgactta acgcctgcag cctgaagtct
     3421 tgttttcggg aggtagtcgc ttgcctttgt tttatcggca gcaacaccgt ttgtgaatgt
     3481 cttagaatca gttgacggct ttgcatactt tgaaagaaca tgctcattga taatattctt
     3541 gagagtattg ctgttcggct tcattgagtc aactacgatt gaaataacct ctttctttgt
     3601 aacggaaagc ttcgaagcct tggctgtggc cttgatagaa ttgttgtagt aagcaactat
     3661 ctgctccttg gaatacttcg tcgggtctgt tgactttgct gagctgccgc ccgatgaggc
     3721 agagccaccg gttgatgtac ctgccgaaga gcccgaactc ggtgtgcttg cgtcaggtgt
     3781 tgccgcgtcc gttgtgccgg catccggtgc tgttgcgtcg ggtgttgccg acgggtcaac
     3841 cgttgaaccg cccgaggagc cgccggttgc tgagcttccg ccgccggatg tgcttgcgga
     3901 tgcaggtgtg tgttttgccg tttcaaccat agcgtcaccg tatttgccga tggcaagcac
     3961 ggaagaaaca cagactgcaa taacgcagat aacggctgtc accgatttaa taatggtttc
     4021 tttcatgttt ttgtaacccc cattttttat ttttagctta cgctaaaaag ctgcattaca
     4081 tattcataac tatgaccgca cagatgatgc acagaacaaa cattatgaaa ttgagatttc
     4141 cggaaatgta gttttctttc tttttgtgga ttccgtcaag caacaccacg gaggcgattg
     4201 caagaataaa aactccgaaa aagattgaag tgaattttcc gaaaaacatt gagataagaa
     4261 aagcgagaaa ggaaatcatt gaaaagattg tgactataag ccacggaacg gttatctttt
     4321 cagtgaaaaa tccagagtcc ggtttcttct tgttgttatc gctcaacatt tatcactcct
     4381 gtctataaaa tgatatcaaa aattccgctt ttcgtcaagg ttctttta
//
LOCUS       DAWBSW010000169         4423 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3440_length_4423_cov_2.461996, whole genome shotgun sequence.
ACCESSION   DAWBSW010000169 DAWBSW010000000
VERSION     DAWBSW010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_4423_cov_2.461996"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1201 a          952 c         1068 g         1202 t
ORIGIN      
        1 tgcgatacag aagataaggc cgaatttcaa aggatccata gttatcgacg ggcttgcctg
       61 agccgcgtcg gaaaggattc ctgttatcca cgggccgaac gcacagccga agtcgccgaa
      121 cattgcaagc attgcgaaca ttgccgtgcc gccctttgga aaggcttttg cggaaaggct
      181 gaatattccc ggccacattg ttgaagcgga aaggccgcaa agtccgcagc ctaccagaga
      241 aacaagcgga atcgggacaa gcgaggtcat aaggtaactg attatgcaaa gacctgcgca
      301 aaggctgaga actcgtcgaa gattaagcct gtcgccgaac gagccgtaaa gaactctgcc
      361 cgaacccatg agcacggcaa acaggcacgg accgagaagg tcacctgtaa atttgctgac
      421 accgagactt ttttcggcaa aggcggacgc ccattgatcc atcgcaattt cgcttgcgcc
      481 ggcgcagagc atgagcaatg cacagattat aaaggtaggt tctctgaaaa gctcggggat
      541 tttcatttcg ctctcgccct ccggaacagg aggaacgatg ggaaccctca taaaggcaaa
      601 taaattgaaa aacggtataa ccgcccagag cataggcatg tatatccagt tttccgtgcc
      661 gatcaatttg attgaaaatg ttgtaataag tacaaccgaa gcctgtcccc agcaatagaa
      721 ggaatgcaga aaagccattt ctccggattt attgctgaga ggtaagccct cgattatggg
      781 gctgatgagc acctcgataa gtcccgaacc gattgcatat atgattatcg ggatcaccaa
      841 tccgatgaag tggttgttaa acgccctcgg aagaataccg agaaggataa gtcccgcaca
      901 ggcaaaaacg tgtgcaaaaa caattgatat tcgatatccg actttttcga tgactttcac
      961 ggaagcaaag tcaataaaaa tctgtgatac aaagttaata agaataagcc agcttatttg
     1021 tgaatagttc agtccgaatt cgtcctgaaa aataacgtaa aaaagtgacg ctaagttgtt
     1081 gattatcgcc tgaacaaagt atcccgcata acaggcgatt ttggttgaag tgtatttgct
     1141 gttagatgtt gtcattttcg ataaggtcct cattttctgt gttgttgtcc ttccagtacc
     1201 accagcggag ttttttctca tccggtttcg gtgtattggc aaaataaacg gctgttctca
     1261 tttcatagag aataacctta tcggcaacat caccgctctc gacgcagtag tcgagataga
     1321 aattatctcc ctcttttccg acaagctgag atgtttcggt gatttcgaga atgttgaatg
     1381 cattctttgc ttcgtcgctg agaaacttca gttcgtcaat tttgaagttc ttcggcgttt
     1441 tgtctttttt tctgaaaaac attttttatt acctccgtag atgtatttta gcataattgt
     1501 aaatataagt aaagagttga aatcaaaata aaaatatggt attattataa cagctttttg
     1561 attcagagga tgaaataaat gaaggttgca caggactttt tgaatacgca tgactttgtc
     1621 attgtcgata tagaaacaac gggcttgaac tgtaaaacgg acgagataat tgaaatttcg
     1681 gcagtcagag tggaaaacga tactgttacg gacgagttct cgaatttagt tcatacagac
     1741 aagaggataa gctcttttat ttatgacctg acgggaataa acaatgccat gctcgaaaac
     1801 gccgacgata tttcaacggt gctttgcaga ctgttcgatt ttgtcggtga tatgccgatc
     1861 gtcggacaca atatcggctt tgatatgcgt tttatttcgt ggaattcggc gttacttttc
     1921 ggcgaaaagc catgcaacaa gacccttgat tcattgctct tttcaaaaga aatccttccc
     1981 gagctgagaa gccataagct gtcttttttg aaggaatgct ttggaataag cggaggttcc
     2041 caccgtgcgc ttgacgattg cagaacgaca tatgaactgg tcgagatact caagaaaaag
     2101 gcggagtgtt cggtttgatt cttgattaaa agaagaaatt ttccaaggct ttttgtcggc
     2161 acaatttatt agatttaagc tttttatctt tctttattcc ttctattaca cggcttgaag
     2221 agcaggagcg gatagagaaa atgacatatg gttaagcagg ggatttttaa aaactgcaac
     2281 aaatatacaa ctttattgat gaaaacactt tacaaaaccg atttcgggtg gtataatact
     2341 aacgttgtgt ttccgctttc tcggattacg ggtcttaccg caatgcggtg ttcacctgcc
     2401 gtaacctggg aggtcggcgc aaaatattat tttaatgagg tgaaaattat gacacaggca
     2461 gagaagcagg ctatcatggc cgagtacgct cttcacgagg gtgacacagg ttcaccggag
     2521 gttcagatcg ctgttcttac aaagagaatc aacgacctta cagagcatct taaaactcac
     2581 aagaaggatc atcactcaag aagaggtctt cttaagatgg ttggtcacag acgtaacctt
     2641 cttgcttact tgcagaaagt tgatatcgag agatatcgtg ctatcattgc aagactcggt
     2701 attcgtaaat aatttcggca ttatgaggca gacagcattc ttttactgtc tgcctctgtg
     2761 tcgatattta aaaagtatct atttgtcggg tagctcatcg gttttagcag tgaatcggca
     2821 gcttgaaact caaactgcgg atttattgct aaatccttgc ccgacgtaga aataaaattt
     2881 ttacagagag gtattaaaaa tgtttttcaa ggatttcaga aaatttgaaa caactcttgc
     2941 aggacgtccg ctcgtagtcg agaccggtaa aatggctcag cttgcaggcg gctcatgtct
     3001 tatccgttac ggagagactg aggttctttg cacagccaca atggcagaca agcccagaga
     3061 gggtgttgat ttcttccctc tttcggttga ttttgaggag aagctttatt cagtaggccg
     3121 tattcccggt tctttccagc gccgtgaggg caaggcttcc gagaaggcaa cgcttgcttc
     3181 ccgtgttatc gaccgtccga tccgtccgct tttcccgaag gatatgcgta acgatgtcgg
     3241 cgttgttgca acggttatgt ccgttgaccc cgattgctca cccgagatta ccgctatgat
     3301 cggtgtttcg gttgctattt cgatttcggc tattccatgg gacggtccga tttcaggcgt
     3361 ttccgtaggt cttattgacg gtgagtacgt aatcaacccg accgaggagc agagaaaggt
     3421 ttctcagatg gcggttacgg ttgcttctac caaggatctt gttgccatga tcgaggcagg
     3481 cgcaaacgag gtatccaatg aggatatgtt cgacggaatc atgtacgctc acaaggttaa
     3541 ccaggaaatc gttaagttca tcgaggatat ccgtgccgag gtcggcaagg agaagattga
     3601 cttcccgtca aacgatccgt ctcccgagat gttcgaggct atcaaggagt tcgccattga
     3661 cgatgttaag gttgctcttg atacagacga caagagaatc cgtgacgagg cacttcgtcc
     3721 gatctacgcc gctgttcacg agaagttcga cgaggtttat cccgacgata tcgcaaagat
     3781 cgacgattgt atgtataagc ttcagaagtt catcgttcgc cgctggcttc ttgacgaggg
     3841 caagcgtgtt gacggacgtg gaatcgacga gattcgtccg cttaacgcag aggttgacct
     3901 cctcagccgt gttcacggtt caggtatgtt cactcgcggt cagactcagt gtctttcaat
     3961 cacaacgctc ggacctatga gcgatgttca gatgctcgac ggtatcgaca acgagaccga
     4021 aaagagatat attcaccact acaacttccc gtcatactca gtcggcgaga ctcgcccgag
     4081 cagaggaccg ggacgtcgtg aaatcggtca cggtgcactc gcagagagag cacttcttcc
     4141 ggttattcct tcggttgagg agttcccgta ctgtattcgt gtcgtttccg aggttctttc
     4201 ctcaaacggt tcaacctcgc agggttcaat ctgcggctca actctttcac ttatggcagc
     4261 cggtgtgccg attaaggctc ccgttgcagg tatctcctgc ggacttatca ccgagggcga
     4321 ccgcttcatg acaatggttg atattcaggg tcttgaggat ttcttcggcg atatggactt
     4381 caaggttgcc ggtacaaaga agggtatcac agctattcag atg
//
LOCUS       DAWBSW010000170         4417 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3443_length_4417_cov_3.149473, whole genome shotgun sequence.
ACCESSION   DAWBSW010000170 DAWBSW010000000
VERSION     DAWBSW010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3443_length_4417_cov_3.149473"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1120 a         1024 c         1063 g         1210 t
ORIGIN      
        1 ctttaaatag atttaggagg ggatcatatg aaaaaaatca tgcaaagagg aattgcaatt
       61 ttgctttcct gcgccctgat tatcggcgct aactttactt ccgtttttgc ggttcagagc
      121 ttttggcaga gaataggaac gggtgcgctc ggaacggtta tttccggtgc actcggagcg
      181 ataaattcaa ttctgcccga cggcaaaaac tttattgccg aggaggatta cgaaagccac
      241 gatttttata agggcaacga cacttttctt tctgctccgt cgcaaaatgc ctgttggcgg
      301 ctcggctaca acagtgtcag ccttgttcct gacgactggc gggagcacca atactatatc
      361 ggcggataca taatggcgga aaattggttc accaacaagg tcgagggcat aatcgacgac
      421 atgaaagccc gtgttatcgc cgttgacgat tcgagcggac gcggagtttc cgtttttgcg
      481 acgattgact gtatcggcat gacaaacagc gatattaaag aaattcgccg ccggcttgtt
      541 gaaaaatcgg acggaaaatt taattttgca acgataaatg ttgcatcgac acattgccac
      601 agcggtattg ataccgaggg aatttggacg aatcttttcg gcaagcttat tccgaatatt
      661 ttcaagctta aaacgggtct cggcgaggtt gagcagggca cggataagca ctatatggat
      721 tttctttttg ataaagtttc cgacgcaatg ctcgaagcgt gcaatagcat gacagaagga
      781 aagctgacga tctcaagaaa agatatcgga gagggctatt tcacaaacaa gaaccgctca
      841 tcggcttccg caatgcttac ggatatgacg gttatgacgt tcactccatt caataagagc
      901 gcaagagcga caaaaattgt aaatattgcg gcgcatccgg acgttgcggg actgccgaca
      961 agcgacggac agagcagcgg acgtgaggtc agcggcgact atgtttacta tatggacgag
     1021 cttatctcaa aagcaggctt caattgtatg ttttttaacg gagcgattgc cgggatttat
     1081 atggcaaggg gactgacaaa cgattcgcag gatttcaacc gccgttggga gcagtccatg
     1141 cgttacggcc atgagattgc aaaaatggcg ctttcgctca atcttacgca agcgcagata
     1201 aagcaaaata agcttctcta tgacgaggaa gaaattaagc gtgagaccga gattgccgaa
     1261 aagaacggcg gcgaatacac gctttggtgt gagggctgga cgcccgttga cgaaacagaa
     1321 gttaagccgt tctttaatat aagaatgaag gagattcggg tgcctgttac caacccgttt
     1381 attctcatgg caggtaagct gaaaatggcg aactatgagg tcatcaaggc ggagaacggt
     1441 tatgagattt ctacggaggt cggctacatg gagttcggcg attcgctcaa ggcggttaca
     1501 gctccgggcg agatttgccc ggacattatc tacggcggca catcgctgac agcttccgat
     1561 tcgtactcgg gcaaggatta tgaatatccg aaagctacgg agatttttaa cagtgacgag
     1621 ctcgtctgct tcggcttgat gaacgatgcc gtcggttata ttgttcccga caatgactat
     1681 tgtatggcgc ttgcgtttga ccattatcac gagcttgttt cgctcggaaa acatatcgca
     1741 agcagcgtat caaaggcata taccgagctt gcaaaataaa taaagacaaa agccataaat
     1801 tacagagtga tccgtaattt atggcttttt cgttgaagaa atttttatat gtaaggttca
     1861 gcagttagca tagattagat atgttttcaa aatatggcat accggttccg ctttcataat
     1921 gtgcaattgt gaaacggtaa agctccatta tgtcgtcatt ttcttatttc gttttttctg
     1981 taacataaga ttttctctct cacaagttca cgtatgtatg ctcgcatact tcattgattt
     2041 tatctctcag cttaagaaaa tcctcaagcg cctgaggctt attgttcgga ttgcgaagca
     2101 gggcagcggg gtgaaatgtt cccatcatga gaaccccgtt cttttcaata aactcgccat
     2161 gctgcttcgt aaccttgaaa tcgggaccga taagccgctg agcggaaatt ctaccgacac
     2221 aaactataat tttcgggcgc ataagcctga cctgctctct aagccaagag atgcaagcgc
     2281 tttgctcctc cggcttggga tcacgattgt gcggaggtct gcatttaacc atgttggcga
     2341 tgtaaatatt ctttctcctg tccagcccga ttgcgtcaag gtatttatcg agaagctgac
     2401 cgcttctgcc gacaaaaggt tcacccttga gatcctcctg ctcacccgga ccttcgccga
     2461 tgaagagaac ctctgctttt tcatttccga cgccgaaaac aagattggtt ctcgtagctc
     2521 caagctcaca tttgttgcag ttgcagcagt ctgttttcag ctcattccaa ttgttataca
     2581 tcggcgcact cctttgcatc tttttttatc tggcgcgcaa gttcatcgaa ggaagaaaat
     2641 ttcctttccg gtctgatgaa tttaatcagt cggatatcga cgctctcacc gtaaatactt
     2701 tcgtcaaaat caattatata tgtttcgctt agaatttttt ccgttccgac ggttggacgc
     2761 ttgccgatgt ttgttacacc cttgtaacgt ctgtttccaa cggtaacgtc ggttcggtaa
     2821 actccgaatc tcgggagcac aagctcctgc ggaagctcct ggtttatcgt cggaaagccg
     2881 agccttcggc ctcttttatc gccgtcaata acaggtgagg aaaaaccgaa tttgtggccg
     2941 agcatttcgt ttgccttttc tgtttctccg ttttcaataa ggcttcgaat ctgagttgag
     3001 ctgacagcat ggcctgcaac atcaacttca cctgcaattt tgcacttgat gccgagcctg
     3061 tcgcagattt tgcgcattgt ctcagccgag cctgccgcat ccttgccgaa gcggtaattg
     3121 tagccgcagc agaccgcccc tgcgcggagc ttttctacaa gaattttttc aacaaaatcc
     3181 tcgggagaaa gattttttat ttcattaaat ataatcgttt tgagtttaag accctttttg
     3241 attatatatt cattgcgttc accgacagtc atcagcatcg gcggtgcaat tccggttatc
     3301 gccttcatgc tgtgctcgtc aaagagcacg gcgacgggga cgagtccgtc cttttggaag
     3361 cttttcgctc cttcaagcac agccatatga ccgacgtgca taccgtcgaa gttgccgagc
     3421 gcaaccgctg ttctgtttgc aattttttcg tctgtcatag tatcaccgtt tgagaaaaat
     3481 gtcattgaac ttaacgagga gtctcccctg attgttaggg cagcgaagtg tcgacacggc
     3541 agtaaatgga cagtccggcg gactgtcaga gccgtggcgt gaccgagacg cagcgaaaaa
     3601 gggggatcgc ttgcggtggt ggaaatttga aataacggtt aacttattta acccctccga
     3661 cttcggcgca atccgccgta gccacctccc ctaacaatca ggggaggctg atgggataaa
     3721 tattatttaa caaagatatt ctcaaatagt cgcttttctg tagcaatatc cgattatttt
     3781 tttctgaaca tctgcttcat gcgctgttcc tttgaaagaa cacgcttacg aagtctgagc
     3841 gattcgggag taacctcaag aagctcatca tccttgatga attccattga ctgctcaagg
     3901 ctgagcgtcg aatggggaac aaggcgaagc gcatcgtcgc ttccggcggc acgggtattc
     3961 gtcatctgct ttttcttaca aacattgcaa acgatatcct cgtttctcga acattcaccg
     4021 acgatcatgc cttcatagac ctcaacgccc ggtccgataa acaaacggcc tctgtcctga
     4081 gtgttgaaaa ggccgtagcc cgtgctggtg cctgtttcat gaacgacgat cgagccgcgt
     4141 tctctggttt gaatatcacc cttgtacggc tcgtatcccg caaagagctg attcataatg
     4201 ccgttgccgt tggtgtcggt catgaactcc gaacggtagc cgataaggcc tcttgcgggg
     4261 atcttgaatt caaggtgagt tgctccgccg ttccttgagc ccatgttctc aagctcgccc
     4321 ttgcgtgagc cgagtttttc gataacggag ccgacatact gttcgggaac ctcaatgatg
     4381 agaagctcaa tcggttcgca aagctggccg ttgattg
//
LOCUS       DAWBSW010000171         4415 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3444_length_4415_cov_2.555734, whole genome shotgun sequence.
ACCESSION   DAWBSW010000171 DAWBSW010000000
VERSION     DAWBSW010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3444_length_4415_cov_2.555734"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1111 a         1039 c         1019 g         1236 t
ORIGIN      
        1 tctgcatccg aaagagtagt tgccggcata ttgtcgaggt ttgccccgcc gccgaagagt
       61 gcctttgaag cctctcgcgc cttggctgct tcttcctcac catggacgag ctttgtaagc
      121 tcaaacgcaa ggatatcctt tgcctcattg agctgtgcgc cctcccaatg atccattgcg
      181 tcaatttcct caagcggaag gaaggtgagc atacggatac acttgagaac gtcctgatcg
      241 ccgacattgc gccagtactg atagaattca taggggcttg tcttttcggg gtcgagccaa
      301 acagcaccag aagcggtctt gcccatcttt ttaccctcgg agttcatcag cagggtgatc
      361 gtcattgcat aagcgtcctt gccgagcttc tttctgataa gctctgtacc gccgagcatg
      421 tttgaccact ggtcgtcgcc gccgaactgc atgttgcagc cgtactcctt gaaaagatgg
      481 tagaaatcat agctttgcat aatcatgtag ttgaactcaa ggaagctgag acccttttcc
      541 attctctgct tgtagcactc ggcacgaagc atattgttaa cggagaagca tgcgccgacc
      601 tcacgaagga cgtcaacata gttgaggtcg aggagccaat ctgcattgtt gaccatcatt
      661 gccttgtcct cgccgaattc aataaaacgg ctcatctgct tcttgaagca ggcgatgttg
      721 tggtcgatgt cctcctttgt aagcattttt ctcatgtcgg ttctgccgga tgggtcaccg
      781 atcatgcctg tgccgccgcc gagaagggcg atgggtctgt tgcctgccat ctgaagtctc
      841 ttcataaggc agagagccat gaagtgacct acatgaaggc tgtcggctgt cggatcaaag
      901 ccgatgtaga aaactgcctt tccgttgttg ataagctcct tgatttcatc ttcgtccgtg
      961 acctgagcaa tgagtccgcg ggcaacaagt tcgtcataaa ttgtcatttt gatgtttcct
     1021 ttctcgtgaa aaatatttag acagaaaaag ccccctgccc ttagggcaga ggacgaatta
     1081 tcgtgttacc acctctgttc accgaaccct cacgggaacg gcctcagcga gtacatatat
     1141 actctgacgt ttaacgcacg cacacggcac agcctactgc aattttcagc cgcgcagctc
     1201 agggatgtat tttggcaaaa ctctcacgct ttctcgcacc ggccgaaagc tctcttggca
     1261 tccttttttg cttacttctt cccgtcaaag ctgttgaaag cattatatat tgtttaaaga
     1321 attttgtcaa gcggttgacg ggaaaaagaa aaaagtgtaa aataatctgc ggttattaaa
     1381 aattaacgga gcgaagaata tgagaatgcg aagaaaaaag aatcttgaca gaagactgaa
     1441 gagctgcggc ggtctgattt tttctatcga gagcgaggat aaaaacttta acacggcaat
     1501 tgaagaaaag gaatatatag accttgaaaa atggtttggc aactccaatc cggttcgcct
     1561 tgaggtcggc tgcggcaagg gtcagttcgc cgccgaaata gctaagcgca atcccgatat
     1621 caattatatt gcggtcgaga aatgcgccaa cgttgttgtt attgccgccg aaagagcgat
     1681 cgaggagagt attcctaacc tgcgttttat caaaggctcg gcggaatatt tgccaaaata
     1741 tcttcccgac ggaatctttg atagggtcta tctcaatttt tcctgtccgt ttccgaagaa
     1801 aaaatacgca aatcaccgtc tgacaaacag aaagttcctt gcgatctttg accgattgat
     1861 gaccgatgac ggagaaattc atcaaaagac ggacaatatg catttctttg aatattcgat
     1921 tcaggagttt tccgaatacg gaatggcaat aaaaaatgtt tcacttgatc ttcacaacag
     1981 tgactttgag ggcaacatcg tgacagaata tgaaaagaga ttttcggacc tcggtcaacc
     2041 gatttaccga cttgaagctt ataaaaggta aacgcttttt cagaaaaatt tcatagctga
     2101 taaaaatcca cactcggaaa atgccgaatg tggatttttt ataatgttcc gttttcaaan
     2161 nnnnnnnnna aattcacgtt tgaaccagac gtccttgacc tcaaattctc tggaatggag
     2221 ataggagaga attcctgcgc tttcgtcaaa catgaacacc agtttgtagg aataaaggca
     2281 ctgcttgcgg tagccgaagt ccttattgag cttatttgtg ccgtacttgc cgtcgccgag
     2341 aagcggatga ccgattgaag cgaaatgtgc cctgatctga tgagttctgc ccgtcataag
     2401 ctcgacctca acaaggctta gctgtttatc gtaatccctg ccgagaacct tatattttgt
     2461 gcggatttcc ttcgtaccgt cgcagggatg tttaagaatt ttaacagtgt tttttttctc
     2521 atcctttaag agatagccct ccaaaagtcc gctgtcacgt tccataattc catggcagac
     2581 gcagagataa aattttttga gatttgttct ctgcttcatc tgagcgttca ttattctgag
     2641 gctttcggcg tttttggcag cgataacgat gccgccggtg tttcggtcaa tcctgttgac
     2701 gagagcaggg gtgaacgaat tctcctcatc gggcttgtac tcgccctttt cataaagata
     2761 tttctttatc cttgtgatga gcgtatcgac atattctctg tcatctgggt ggcaaagcaa
     2821 gccggcgttt ttatcaagga gcatgatgtt ttcgtcctca tagagaatgt ccagtcttct
     2881 cggtgcgccg gtgaaatcgt aacgtgtttc gggcttggca aaaaactcgt cattgatata
     2941 catatcaaca acgtcgccga cctgcagaat tgtcgatatt tcggcacgct tgttattaac
     3001 cttgattcgc ttttttctta tgtatttata cataagcgac tgcggcagat tcggcatgcc
     3061 ttttcttata aacttgtcga gccgctgacc tgcattggct tttatttcaa aacttttcat
     3121 atcttaattt ttaaacgagt gtatcggtgc aggaattctg ccgcctctgt taatgaaatc
     3181 cgaacagtct gcaccgttga ccggcattat aggagcatag ccgagcaaac cgccgaattc
     3241 aacggtatcg ccgacgtcct tgccgattac gggaatcaat ctgaccgccg tggttttttg
     3301 gtttatcatt cctattgcca tttcgtcggc aattatgccg gaaatcgttg aagcttttgt
     3361 attgccggga atagcaatca tgtcaagtcc gaccgagcaa acgcaggtca tggcttcaag
     3421 tttttcaagt gtgagcatac cggcattgac cgagtctatc atgccctgat cctccgaaac
     3481 ggggataaat gctccggaga gtccgccaac gtatgacgaa gccattatgc cgcccttttt
     3541 aacctgatcg ttgagcaatg caagagccgc agtagttccc ggagcgccga catattcaag
     3601 acccatttcc tccaatattt cagcaacgct gtcgcctatt gcgggcgtgg gagcaagcga
     3661 aagatcaata attccgaacg gaacgcccag cctatgtgat gcttcacgcg ctacgagctg
     3721 acctaccctt gtgaccttga aagcggtttt ctttatcgtc tcgcaaagag tgccgaagtc
     3781 gccgtcctta acctcactga tggcacgttt aacgacaccg gggccgctga caccgacatt
     3841 tataatcgtg tctgcctctg tcacaccgtg gaatgcacct gccatgaacg gattatcatc
     3901 gggtgcattg cagaatacaa cgagctttgc gcagccgata gagccgctgc tctttgtcag
     3961 ttcggcggtt ttaactataa tttcgccgag cagcttgacc gcatccatat caataccggt
     4021 tttagttgag ccgacattga cggagctgca aactctttcc gttgaagcaa gagcttcggg
     4081 aatggattcg ataagcagct tttcggcagg ggtcattccc tttgaaacaa gggctgagaa
     4141 gccgccgatg aagttgacgt ttatctcatg tgctgccttg tcaagagttt ttgcgatttt
     4201 tacaaaatct cccgtggtat tgcagcaagc tgcacctata agggaaatcg gtgtaacgga
     4261 aacacgcttg ttgactatcg ggattccaaa ttcaagtgca atttcatcgc ctgtttcaac
     4321 gagcctgcct gcttttgaaa gtattttttt gtagatatta tcgcaggttt tgtcaacctc
     4381 tgacgtaatg cagtcgagca ggcttatgcc catgg
//
LOCUS       DAWBSW010000172         4413 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3446_length_4413_cov_2.491510, whole genome shotgun sequence.
ACCESSION   DAWBSW010000172 DAWBSW010000000
VERSION     DAWBSW010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_4413_cov_2.491510"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2309..2318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1011 a         1061 c         1083 g         1248 t
ORIGIN      
        1 accataattt caccgctttt gaagcctcgt ttaacgagca cgtgacgcaa aaatcccgtg
       61 cctctgtcct cgttataagc cgtcatgcga tatttcggca tcattgaacg aatcgtcacg
      121 atgatttcat cggctttcct gtcttcgatt cggcagcggt cgatgcagac tattctgtgc
      181 gactttgact gatagacgcc cgagattatt ttgccgcccc ttgttgagcc gataatggaa
      241 cgggtgctcc attccgatta tgttatcaac atggcagtat ttgccgagaa gcttgaccac
      301 cttcgcttcc ttaaagccaa gctgacgttc atatgtcatg ttttgcagtt ggcagccgcc
      361 gcattttccg gcgaccgggc aaaagcttgt tttttgttct ttctttgact ttttatcgtt
      421 catttctgag gttccttatc aattattttg cagacgccga cagttactgt gcccagcagc
      481 gcaattatga cccaactgat tattgtgccg ttccagccga agctgtccga aagcagggcg
      541 aagccgtaag ccgaagccgc cgagcctaca tatgtcatga aattcagcgt accggaaagc
      601 tccgatacat tttcgttgtt gccgtaaatt ccgggaataa agctgatgag aacaaggtta
      661 actccatgca tgcagccaac gatgagtgcg cacataatcg cagtcagaat tacggaatga
      721 ccgccaaagg cgacaagaat tatcgttgaa gcagcgccga cggcgaaaac catcagtccg
      781 catttgaacg gctttctgcc tgttttgtta taaagcttgg agcagatttg caggctgatg
      841 tatgagaaaa tcggaatgac cgcactggta agaattgatt tttcgctgct cagtccgaat
      901 gactcataaa tgtaggttgg aagccatgtc gttacgccgt cgcggagaat tccctggaag
      961 aatatcgccg caagcgttaa aatgagcaac atgagcggca tggctgggct gtgcattttc
     1021 tttttcttgt cagaggattt tttgagaata tatttgatgt ctgccgacct ctctattttt
     1081 gtcagcacgg cgaaccatgt aattgccatg ataatgccgc aaagcgcagt gataaagaat
     1141 acacccttcc agctccaaac ggagataacg gcaggggaga gcagataaat tataattgtt
     1201 gcgaccgtac ttccgagatt tgtctgaacg cagcctttgt tgaaatcatc ttcgttcatt
     1261 gccgttttca ttatcttgag catcggcggc cacatgagcg actgtgccag tccgttcacg
     1321 ccccaaacaa ggcacatcgg aaccgctccc ttgacaaacg gaatgagcag attcatcgcc
     1381 gctgttgaac agaaaccgat agtcatcagc tttttgggat caaaacggtc gccgagcttg
     1441 ccgctgacga gctgacctac gccgtaggta ataaatccga cggtggtgac tgcgcctgcc
     1501 gctgcttttt caatgtagcc gtcggagatt atcgctgcaa ttacggcggc gaaatttatt
     1561 cttgtaaggt agctgacaaa atatgcaagg gagcatagat atattagctt ttttgagttt
     1621 tttctgtcga gcttgttcat tattcaatgt ccttgggctt tttcattgtg agtgaaattc
     1681 ttttcttttc gatattaaca tcaagcaccc aaaccgtgac gatctgtccg accttgagta
     1741 cgtcggacgg gtgctttata tagcgatttg tgatctgcga aacgtgaacc agtccgtcct
     1801 ggtgaacacc gatatcaatg aaagcgccga agtcaataac atttctgacc gttcctttca
     1861 gctccatgcc gggcttgaga tccttcatat ccataacatc tgtacggagc atcggcggcg
     1921 gaagctcatc acgcggatcg cggccggggc gcataagctc cttagtgata tcctgcattg
     1981 tcgggacacc tacgccgatt ttttcggcaa ccttttcttc gccgagagcg gtaatctttt
     2041 cgccgagatc gccgattttg ttttttgcgg cgtcattgac tgtgtagccg cagatttcaa
     2101 gcagagcctt tgcggcggta tagctttccg ggtgaacacc cgtgttatca agaagatttt
     2161 tgctctccgg tacgcgcatg aagccggcac actgctcgaa tgcctttgcg ccgagctttg
     2221 gcactttttt gagctgcgac cgagaagcaa attcgccgtt ttcctcacgg taagcaacga
     2281 tgtttttggc aattgaagca ttgatgccnn nnnnnnnntt ttttgcaacg gtcgagttga
     2341 ttccggaaac ccttgtcaaa agtgaaggtg aagctgtgtt gaggtcaacg ccgacgctgt
     2401 ttactgcgtc ctcaacaacg ccgttcagtg cgctgtcaag ctccttcttc ggcatgtcat
     2461 gctgatattg acctacgccg attgccttcg ggtctatctt aacgagttcg gcaagaggat
     2521 cctgcaaacg tcttgcaatg gaaaccgcac ttctgagcga aacgtcgtac tgcggaaatt
     2581 cctcggcggc aagctttgaa gcggaataga ccgatgcgcc ggcttcgctg actaccatgt
     2641 aagcggtgcc ggtgtcaagc tccttgagaa gctcggcggt gaaaatctcc gtctcctttg
     2701 atgccgttcc gttaccgatt gcaataatct gcgcaccgta tttctttata aaactcttta
     2761 taagtgcctt tgcctgcgcc tgctgagcgg cggagtgggt gatataagca actccggtat
     2821 cgagcacacg tccggttgcg tcaacgactg caaccttgca gcctgttctg tatccgggat
     2881 caagcccgag cgcaaccttg cccttgacgg gaggctggag caaaagctcc tttaaattga
     2941 gcgagaaatt tctgattgcc gaagcgtcgg ctctctcggt caatgcggag cgaatctcac
     3001 gctcgaccga cggaaaaatc agtctgtcgt atgcatcaac gcctgcgtca cgaaccgtgt
     3061 ctgtacaggg cgagcctttg tcgttgagcg tttcggaagc aatgacattc aatgccttaa
     3121 cccggtcaag ctcaaggctg accttgagaa atccctcatt ttctccgcgg ttaattgcga
     3181 gaattctgtg gtcggcaatt ttgctgacag gctcggagta gtcgtagtat tgctcataaa
     3241 cgctctttgc ttcggcgtcg cttgctttta catttactat gccgacaacg ttgagaagat
     3301 ttttcagtct gcggcggata tcggccatat ccgaaatgtc ctcggcgatg atatccattg
     3361 cgccttggag cgcgtcctcg gcggtgttga cctctttctc cgcgtttatg tagtccttgg
     3421 caagatcaat agcggacggg ctgtttgctg tctgaagata gatagcctga gcgagcggtt
     3481 caagtccctt ttctcttgcg acggaggctc tcgtctttct cttgggcttg aacggacggt
     3541 aaatgtcctc caattccgca agagttgccg ccttatcgag tgcggcggag atttcatcgg
     3601 tcagcttttc ctgaccctcg atcgatgaac gtatctcctc gcgccgcttg tccaaattgc
     3661 gaagatactc cagtctttcg gagattgcac gcagggtttg atcgtcgagt gtaccgtgag
     3721 cctcttttct gtatcgggca atgaacggaa tagtgttacc gtcgtcaata agcgagacaa
     3781 cattttcaat ctgccatttt tttaagttga attcggctgt aagtgtaaga attatatcca
     3841 taaattacct cgttttaatt tgtcttatat ctgtgccgtc gaaatagagc atgacgtagt
     3901 tgctgagaat tcgagaagtg attctttgac tgtatgtttt ttcaagctca tcaggaagaa
     3961 ggtttgtgct tataattgtt gatttgccct caagctctct cgaattaaca atgttgtaaa
     4021 gcgaggaaat cgtgaactga gttgtaaact ctgctcctaa atcgtcgatt atcaataggt
     4081 cgcaattaag tatattttct tcggtgttgc cgtcggaacg gccgaatttc tctttttcga
     4141 gcttgttgaa aaggttttgt gccgaggaat agatgactcc gtagcccgct tcaacaaccc
     4201 tgcccgcaat ggcaagagaa agatgagttt ttccgagccc cgtatgaccg tagagcagga
     4261 gacttgatga ttttcggttg aaatcgacgg cataggtttt gcagtaattc agcacgcttt
     4321 ccattctttt gcgcgggctt gtgccgaccc cgtcggggtg atagtcgagc ttgaagtttt
     4381 caaaccgaca gtcgttgaca ggcagctttg agc
//
LOCUS       DAWBSW010000173         4381 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3474_length_4381_cov_3.028895, whole genome shotgun sequence.
ACCESSION   DAWBSW010000173 DAWBSW010000000
VERSION     DAWBSW010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_4381_cov_3.028895"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1158..1167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3652..3661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1204 a          984 c          996 g         1177 t
ORIGIN      
        1 gccgaaggta aaggcgccga ttttaagaaa ggtgagaaac aaagtccaca cttttttcat
       61 tgccgcacct cctgtctttt acgaataata aaaagtataa tgataccgag aataatctga
      121 actgccccaa attgtgcaga cagtataaaa agaccctact cgatgtagca gaataaaaat
      181 taagcaacaa gctgatttcg tttttcaagc ggagtcagtt ttgttttagt ttgaatacgg
      241 tagttgttgt agaagttgat gtattcggat atgatgaggc ttgcctcatc atatccggaa
      301 agtttaactc tgtggataca ttccgttttg agaattgaaa agaaattttc ggctaaagca
      361 ttgtcatatg ggttaccacg ccttgacatt gacggcgtaa tctgtacatc agatttaatc
      421 agagcttcct tagaaaagta taaatatttt acaaaaatta taacacaccg cattatgaaa
      481 agcaatcacc tattttaaaa agtttcttta gccatgagca aaacgtcaaa agtgtagata
      541 tatcaacgct tttcgggcat ggcaagcccg atttatcact ccaaaatata gaaagcaacc
      601 acctaatatg gtataattta attgtagaaa aaccaaccaa ggaggcggtt gctatctatc
      661 gtcaggaaat tttgcaagac attcgtcttt tcacttcaca gcctaaagct aagttttacg
      721 ataacctttt ttgaatctcg aattacccga aaatttctgc aaaggtaaca ctacaggttg
      781 caaaggattt cctaaatatg ccttgctatg tgccttcatt gttatgaaat gtgaaggctt
      841 ttctcaaata accggaaaag tcaaggcaca gcttgagcag ctccgatcca caaaggcagc
      901 acgggaagcc caggcagatg ccatcggagc tttcatgttc gagatgcagg aactggacat
      961 tctcaccgag tttgacgaaa agctctggtt gacagtcatt gacacggtaa cggtccacac
     1021 tgacggacgg atgagattca aattccaggg cggcacagaa atcgaggctt gagtcccaac
     1081 aaaaatgaaa agaccgcagg tttcaccgcc tgcggtttat tgctgtcctc acgggtagag
     1141 taatgcaccc cctcaaannn nnnnnnnaaa ttagagttat ttgccaaaac gaaaagcagg
     1201 tcgcaaggcc tgctttttgt tttgtcaaaa ataaataaag ggattcgaac aaggagggag
     1261 cagacacagt ctgcggaaga aaacagtccg gcggactgtt tttgccgacg tggtcaagga
     1321 gcgaagcgac gcgaaggcgc acgggagtgc gccgaaaaaa tcccttgctc tccgccataa
     1381 agcccaccgt aatttcgata gaattacgat gggctttttc tatgcccgaa aaccgcttaa
     1441 cataagggta tagggcgata ttttttagtg tttttacatt cgaggcagca tatataaccg
     1501 acggtcgagt ctttcaggga ctaaagcgct tatgtcaata aaaaatatac ggttaatcgg
     1561 cggacgatcc tgtcggaatt ctcacagcta ttgttgtgcc ggcatccgaa ctttcttcaa
     1621 cccgaacatc accgccgtgt agagcggcaa tttcccagac aagcgacaga ccaagtccta
     1681 caccgccgta ttcgcggctg cgggatttgt ccacacggaa aaagggctga aaaatgcttt
     1741 ctcgatactg ttcggggata ccgtgccctg tatcagatat acggataaca agatgcgtcc
     1801 cctcttttga cgttttaaca ttcactgttc cgttcgggcg attgtaccgt atggcattct
     1861 cggtcagatt aaaaatcagc cgatatatca gcgtatcgct gccggtcata acaccgtttc
     1921 cgcaaaattc gagagcaata ccgtttttgt ccgccagcgg agaaagatcg gcaaaaattt
     1981 cctcaaccat gggagctatc tctatttggt cggtacacgg aaccgtgcga agctcgctca
     2041 tctcaaggag agttttcgtc atttgagaca ttcgctccgt ctgttcacgc agcaggcaaa
     2101 gaaaatccgc cgtttccggc agcacatcgg gatgctctgc ggaaaaaaga tcaagctgag
     2161 cctgcataag cgcaagcgga gtgcgaagct catgggctgc atttccggtg aactgccgtt
     2221 gagctgtaaa accctcgtcg agacggtcga gcatactgtt gaatgatatg ctgaattgtc
     2281 taaactccgg cagtacatcc tcggttattt tcatatcggt aaggttgttc atctgaactt
     2341 tttcaacttg agacgcaaag gttcgcaagg gcttcagtga acggccgctg aaaaaatatg
     2401 ccaatacacc gccgataaga gtcaccgctg ccgtaatgta ccagttagta gcgataaagg
     2461 aatcctgcac accgtgtata acaatcgtca gctctttatc cgtcttttcc tgttccggat
     2521 caaagaaacc cggttctccg gcgtctgtgt tgctgtaggc cgaaacatag gaaccgatgg
     2581 aatccatata atgtctgccg gaaaagctta aaaggcagtt catggcaacg caggtcacgc
     2641 cgattaagac ggcggtcatc aaagtgatgc gccattgcaa agaaagcttc ctcataagat
     2701 gttgtcctcc ataacatatc cttcaccgat acggttgcga atagggtcat accctagagc
     2761 ggcacgtaat tttttgcgca gagcggaaat atgtacccga atcgaattac tgaaattgtc
     2821 cacgctgtta tcccaaacat ggtcaataag ttcttcctga cttacaggac gtccccggtt
     2881 aagcaacagg tattcaagaa ttgccgactc ttttcgtgtg agcgtcagtt taagcccggc
     2941 ggcagatgct atgcgtgatt tggtgtcaaa agaaatatct ccgcatgtaa gaaccacgtc
     3001 gttttgcgtg aactgtcgcc gtgtcaggct gcgtattctt gcctctaact cttctaaatg
     3061 aaagggcttt gataaatagt catttgctcc ggaatcaagc ccgtccactt tatcggctac
     3121 ttcactgcgt gcggaaagaa tcagaacctt tgtgtctctg tctgtttgac gaagcttgcg
     3181 caacacagtc atgccgtccg tgcccggcag atttaagtca agcagtacaa ggtcatagct
     3241 ctcaatgctg agaagctcaa tcgcttcatt tccgtcatag caagaatcta cactgtaatc
     3301 aagacggcgc agactgcgta caatagtttc gcacagcacc ttctcgtctt caacaactaa
     3361 aatatgcata aaatcctcct cactttttat ttttcatctt atttaacgat tttattatat
     3421 catacaaaca taagattttt gttaagtttt tattcttaac gcagatttta tatactcggt
     3481 gctataatga gggcacaaaa gaaaggggtg acttcattga ttcgatcaaa aaaagccttg
     3541 cttcaaattg cattgcttat cggtgcagcg gcaatgattt gtttcggagc cctgcgcggc
     3601 gaggcggaca ccgtgcttag caaggcgatc agactgtgtt tggagtgtgt cnnnnnnnnn
     3661 ncggcagcga cacttctaac caacatccac ctgccgaatt ttttaaaggg cggaatttat
     3721 caaggaaaag gaaaagccgt ttgtgtcccc ggactgaatt gctattcctg cccggcggca
     3781 tcgggagctt gcccgatagg ctcgtttcaa gcggtggttg ggtcgtcaaa atttgctttt
     3841 tcgtattata ttacgggatt tcttattctg ctgggcgtgt tgctgggacg attcatctgc
     3901 ggtttcctgt gcccgtttgg ctggtttcag gagttgctgc acaaaatccc gacacgaaaa
     3961 atttccacaa agaagctgaa aggacttacc tacataaaat atgccgtttt actcataacg
     4021 gtagttttac tgcccgccct tgcagtcaat gacatcggaa tgggggatcc gttcttctgc
     4081 aaatacattt gtccgcaggg ggttctcgag ggagcaatac ctcttgctgc cgtcgattcc
     4141 ggactccgtg ccgcattagg gtcattgttt tcgtggaagc tcggcatcct gattgttacg
     4201 attgtgctga gcgtgctgtt ctaccgtccg ttctgcaaat ggctgtgtcc gctgggcgca
     4261 ttttatgcgt tgcttaacaa agtatccttt ctcggaatga aggttgataa gaacaagtgt
     4321 gtctcatgcg gaaaatgtgc aaaggcgtgc aaaatggatg tagatgttac caaaagccct
     4381 g
//
LOCUS       DAWBSW010000174         4331 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3523_length_4331_cov_2.298176, whole genome shotgun sequence.
ACCESSION   DAWBSW010000174 DAWBSW010000000
VERSION     DAWBSW010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_4331_cov_2.298176"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3780..3789
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1399 a          896 c          959 g         1067 t
ORIGIN      
        1 gccatgaggc gaacgaaaaa gaagttctca gttttgtaaa gaacagtcag tatgcggata
       61 aatacgattt cctctgcgaa tctccccttg tcaatgcggg cggtgcggag ctttatgcgg
      121 tagtaattgc cgatgttaaa tacagtgcat ccgtttaccg ggcggaaata acagataacg
      181 gtgaatattc cgtaaagacc gacgatgcgc tctataatta cagcggaaac ggaattgatt
      241 attttttact tcgctgcaat gaaagtgaaa tacactcaaa tgtatcgctg tattttaaaa
      301 aaggaaacga ctctttttcg gtattcccga tgctgtccgg aatggacgga catcttgtcg
      361 cggaaaaatt ctatgatttt tccgtataca cagattacgt caacaacagc gaacccgagg
      421 atagaaacgt tgaaattgcc cgtgagattc tttcggagtc tcaggaggtt caatactata
      481 taaataaagg catgagtgtg caatatacag gagagatccg tgaaattgac ggccgtccat
      541 gctggatttt tgcactcggc acagacagag acgaccaatt cgtcagagaa cgttattacg
      601 gagtttgtga caaccttata tatttttatg atacgttgaa tgacaaatgg gaagttctcg
      661 gaagaggata attctttcga acctctctat aatgtgactt accgagtcat acgcggcagc
      721 attcggagga gcttttaaaa agctctgttc cccgagtgcg attatccgtt gatgataaat
      781 acaaacaaaa ataaaaggtg atttattatg aaaaaactaa aaataatgtc ggcaattctt
      841 gctgtgatta tgacattcgg gctttgtgcc tgcggcaaag acggaggaaa ttccccttcc
      901 gacgccccga caacttcggc aacaaccgtt gctcctaccg atacggctac aacaacagcg
      961 gcggcgtcag ccgacgagta tatcactacc ggaaagagcg taaaaattac aattcccaag
     1021 cttgaaaata ccgtcaaaat tgataacgca aaatttgaaa gctccgttgt tcctgacgga
     1081 aaatacgatt ccttcaactc gacaattacc gtcgaaggaa atgacaaaaa attcaatatc
     1141 atcattaaag acggttccct catgggtaaa acaagccttg aggttgccga aaaggtgaat
     1201 aaaggtgaag aagatgatga taaaaagtat aaagcttgcg aaattgccgg ttttaagggc
     1261 attttaaaag tcaatacata ttccggaaat tcaaaaacgc agcatatata tttccttgat
     1321 gttccttgcg gcgatgaaac atatgtcgtt aacttcagac ttgaaaaatt atatgacgac
     1381 gacgtttccg aaatgaaaac aatcgaagaa gctatattaa agagcataaa ggtagaaaaa
     1441 agtaattaag cgcatttaaa gggaggaaca ttttatggga ttgtttgata aaaaattctg
     1501 cgacatttgc ggtgaaaaag ttaacatgct tactcaaaaa aagctttccg acggtcagct
     1561 ttgctccgac tgtaagcaca agttaggctc gttcacaagc ggctggaagc agagaaccgt
     1621 tcaggacgtt aaaaatcacc ttgaacaaag agagcagaac aagcaaaagt atcagcagtt
     1681 taactgcact gcaacagcag gcggaagata cagttcgctt caagtcgatt tcaatcaccg
     1741 ctggtttatc tttgctattg ataacagaga ttttaaaagc ggcaatcctc aggtgtttga
     1801 gttttctcag cttcaggatt tctggatcga accggaatac agaacgcttg acgattcgga
     1861 caacgacggt atccccgaca acagggataa ttttgacaac agacagctta acaattctaa
     1921 cagcttcggc ggaaatatga acagaatgaa taatatgatg aattcatcaa tgctcaatat
     1981 tccgcttgcc gctcagccgt ttgtacgtaa ttcaagctca tactcttcgc caaacgaaat
     2041 acgcgaggtg tgcaatgtga gagtatactt ccgcgttaca gatcagttta taacgaaccc
     2101 gatcggtttt gatgtaacgt cgggaatttc ctcaggaaat caaatggagc ttatgaatgc
     2161 ttatgagact gctgtccagg tcatgcagct ttgccagcaa atcaagcaag gcggagctcc
     2221 cgttaaccaa ggcttcaatc aaatgaatca gggctacggt cagccgcagc aaaacatggg
     2281 cggatttaca ggaagcccgg ttcaaaatgc cgcctacgcc gctgcttccg ctcaggttca
     2341 aaacaacgcc tataataatg ctgcacccgt tacaccgcaa acagttgacg cttcctggat
     2401 ttgctcatcc tgcggcacac gcaacgaagg caaattctgc caaggctgcg gctgtgcaaa
     2461 gcctgccgca atgccttcac gctgcccgag ctgcggatgg accccggcaa acggtcagcc
     2521 tatgccgaaa ttctgccccg aatgcggagc acgcttataa tttgaagcga gcagactgca
     2581 ttaaaaaagc gagcttattt ttgcttatgc tgttattacc tataggtctt ttcggctgcg
     2641 gagcaaagaa aaaatacgct gcggcggatg tgagtgtaat cagtttttcc tgttcgtcaa
     2701 tgagctacac ggattcttat gtttattctc tcaaaaaaga aaatgaagaa tggctttttg
     2761 atgcaaacta ttcctacgac tatgaaaatc caagggtcga atttgagaat aaaaaagtaa
     2821 gcgcacagga tgccgcggca atacttgacg ttgtgaaaga gcaggacctt attttacagg
     2881 ctcagaagta taagcctccg cgaatcaaag cctttgttct cgacggcggc ggatactatc
     2941 tttattttaa gatgaacgac ggcaaagaaa tcaatgcgga aatatataac gaaaatttgg
     3001 taaatgaatt acacactttg gcagaaaagt gccgaaaatc ataacctacc gtgtaaacta
     3061 aagcacggaa taatatattg gaggaaaaaa ctatggcaga cattaaatca attgcaacgg
     3121 caacagcccc gagagagttt gaactctctt attcaaccac cgttgaagag gtatacgaaa
     3181 agctcagcgc acgtgcttcg gcttttaaaa tgcccttcaa aatcaaagag ggaatccccg
     3241 gaaagcgcat ttcttttgag aaagaaccga acctcgatgt tacgatttgg gtctttgtca
     3301 aggacggcaa taaaatcaag gtcatggcaa acattcagga gaacaaaacg accgtcaacg
     3361 gtatgcgcgt tgacaaaaac agcgtattgc aaaaaggcat aaaaggtgta gctaatcttc
     3421 ctattgaaag aggcgaatat cttgacgaag tgaccgaaaa ggttaaaaag atactcaacg
     3481 gcgagcaggt tgaagattat gtagcacccg ttggtgccaa cggcggacag gccgaaaaag
     3541 attggcttgt agctcttctt ctctgccttt tcctcggcgg aatcggtgca caccgtttct
     3601 atgcaggcaa agtaggatcg ggtattcttt atattctcac cgccggcgtt ttcggcatcg
     3661 gtgttcttgt tgacctgata aagatcatta ccggcaaatt caccgacaaa gacggcaatc
     3721 ccattcaaaa aaaataaaaa tttaaaaaag agaaagggtc gattaacatg aaaaaaatcn
     3781 nnnnnnnnna ctcatgattc ttactgtttc tttgagcctt tttgcttgcg gaaaggctga
     3841 cggcgacggc aaggaaagca caacaggaaa tactatcgca gttgataaga cagaagacac
     3901 aacagccgaa gtaacaacgg cggaaggaat gaagaagggt gacgaaggca acggacctgc
     3961 agatatcaac gcagagttta tcactcttaa acttccgtcc ggctacaaat atgaggttga
     4021 ttctttcagt aaagattcaa aaaatccgct caagggcaat gttactctgt ttatttataa
     4081 ggatggctca tactcaccta ttgcgactct taccgctaca gcccgaaaca tggttcgcag
     4141 tcaggaagag gctgttgaaa acacgataaa gctttgcaac cttcagacct ataaaaacgg
     4201 aaaatctgaa atcggcaaag atgctaaata cggtgaaaac acctattcga ctattcatgt
     4261 ttcaacagac tactatgaga aggacttctt tgttacatat gcaaacagag gtgagtcgga
     4321 taagaccgga c
//
LOCUS       DAWBSW010000175         4208 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3639_length_4208_cov_2.747171, whole genome shotgun sequence.
ACCESSION   DAWBSW010000175 DAWBSW010000000
VERSION     DAWBSW010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3639_length_4208_cov_2.747171"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1195 a          989 c          935 g         1089 t
ORIGIN      
        1 atttacggtt cttctcagag ccaattacag attcggacga atgtgccgat gttgacataa
       61 tcgtaaaaaa ttgataaaat aacaataatt tctaaaatga aaggtagata tatcatgaaa
      121 aaaatagttt cacttttact cgtagcagtt ctcgctctca gtctttttgc cggctgctga
      181 tcatcaaaaa gcggcaatat cacaatagcg attccgaacg acaccaccaa tgaagcaaga
      241 gcgctgctcc ttcttcagga aaagggctac ataaagctca aggacggcgt aggcatcacc
      301 gcaacgatca acgacatcgc cgagaacccg aagaacattg agttcaaaga gattgaagcg
      361 gctcagcttc cgaacacact caaggacgtt gactatgcaa tcatcaattc gaactacgct
      421 atcgaagcaa agctcaaccc ggtaaaggat tcgcttatta tcgaaggttc ggcttccgcc
      481 tacggcaata ttcttgcagt taagcagggc aacgagaacg ctccgaaaat caaggcactc
      541 aaggctgctc ttgaaagcaa gcaggttgcc gactatatca ataataaata taacggttcg
      601 gttgtcagcg ttgttgagaa cccgggcgac ggatatgatt catcggtaga ttacaatgcg
      661 ctcaagggca caacgatcac tgttgcggcc tcccccgctc cgcataagga aatccttgac
      721 gtagcaaagg aaatccttgc cgcaaagggc gttacgcttg acatcaaaga atttacggat
      781 tacgttcagc cgaacaacgt tgttgaaaac ggtgagattg atgcaaacta cttccagcac
      841 actccgtacc ttgatgactt caacaaggaa aacggtacgc acattgtctc ggccgcagtt
      901 atccatgttg agccgatggg tctttacggc ggcaagcaga caacacttga cgcattgaac
      961 aaataagtgt agaataagaa gtgctgttcg caacaggcag cacttctatt ttatgtggag
     1021 tgaaataaat gattgaaata aaacacctga ctaaaacctt tgagacctcc gacggaaagc
     1081 ttgatgcgct caacgatgtt tcgttgaaaa tcgacgacgg cgaaatatac ggaataatcg
     1141 gaatgagcgg tgcaggcaaa agcacgcttg tccgctgtat caatatgctt gaacgtccga
     1201 cacagggcag cgtcattata gacggcgttg acgtcggcag cctcagcgat tcccagcttc
     1261 gcaatgagcg cagacagata acgatgattt tccaaggctt taatctgctc atgcagcgca
     1321 catgtcttaa aaatgtatgc ttcccgatgg agcttgcggg agtaaaaaaa tccgaggcag
     1381 cagcccgtgc aaaggagctc cttgaaaccg tcggactcgg cgacaagctc aaaagctacc
     1441 ccgtacagct ttctggcggt cagcagcaga gagttgcaat agcaagagcc cttgcgactc
     1501 atccgaaaat tcttctctgc gacgaggcaa caagtgcgct tgatccccag actacccaat
     1561 cgattctttc gctgatcaga gatatccatg acaagctcgg catcacggtc attgttatca
     1621 ctcatcagat gagcgttgtt gagcagattt gtacccgagt tgccattctc gaccgcggaa
     1681 ccgtcgttga ggaaggaaag gtcagtgatg tttttgccgc tccgaaatcg gacgccgcaa
     1741 agtcattggt ttatcccgac ggatgtgaat tcaatgcggc agattatccc gacgaaagca
     1801 ttatacgtgt tgtattcaac ggcgcttcgg caacaaacaa gcccctcatt gctcagatgg
     1861 caattgacga gggcatcgcc gccgatattc tttcggcttc gacccgttcg cttgacggaa
     1921 aggcttacgg caatatgctt ctgggaattc ccggcggcaa aaccggactt gaaaaagcaa
     1981 tgaattatct gaataacata cccgatatct acgttgagga ggtgacaaaa catgctgagt
     2041 gagtaccttg cctcggaaga ggttcagaat gcgcttgaag tgcttaaaaa tcaatttccg
     2101 cttgcaattt gggagacggt ctatgtaacc ctgctctcca cactttttgc aataataatc
     2161 ggtctgccgc tcggcgtgct tcttgttgcc ggagagaata aaaaaatcct gcgtattccg
     2221 cagggcgttc tccacgttat caacgttatc ataaaccttt tgcgttccgt tccgttcctc
     2281 attcttatga taatggtttt tccgctcaca agactcattg tcggaacggt cgtaggcacg
     2341 cccgcaacgg ttgtgccgct cgttatcgct gctttcccgt tcgttgcgcg actggttgaa
     2401 agcagcctga gagaaatcaa tccgagtatc attgaggcgg cgcagagtat gggcgcttcc
     2461 ccattccaaa tcatcgcaaa agtcatgatc cccgagagcg ttccgtcgct catttcaaac
     2521 ggaactatcg ctctcacgac tgttctcggt tattcggcaa tgagcggaat tatcggagga
     2581 ggaggccttg gcaagatagc cataaactac ggttattaca gatatcagta tcttataatg
     2641 gtattcgccg ttgtgttcct gataatcctt gtgcagatct tccaaagcct cggaactttt
     2701 ctcgccgcaa aatgcgataa gagacttaaa aacagaaaat aaaatttcaa taaaacagct
     2761 ttgcaaacaa aaatttttta tttgcgtggc tgtttttctg ctatattgtt atatataaaa
     2821 aaagcattaa atgttgactt ttgttgattt ttatgattaa atattactat ataaactgaa
     2881 gtagtatgcg tatcgaggtg ttgaaatgaa gtttatcaga attctggtct taatagtatt
     2941 tgttcttgtc gtaggcgcat ggtcagtcaa cgttgttctt gataaaaccg tcagggacca
     3001 cactcccccc gttataacct cggaccgtga tttgcttgag ctttccgtca aagacgacaa
     3061 aagcaagctg gtctcgggct taaaggcaac cgacgaccgt gacggcgacc ttacttccga
     3121 aattcttgtc ggctctcatt caaagttcat ttccgaaggc gtgtgcgaca taacttatct
     3181 cgttttcgat tcgagcaaca atgtgggcaa atatacacga cgggtaaaat atacggatta
     3241 taccgagcct gtattcacac ttaaggaacc ccttatgttc aattccggcg acacggtttc
     3301 catgttctca aaaatcagcc tgaccgattg tcttgacggc gacatttcct caaaggtcaa
     3361 gatcgttgtt aatgacgttg acaattccaa gctcggcaca tactatgtta cgtttgaagc
     3421 ctccaacagc tatgccgatg ttgttaaccg aaagcttccg gtcaacattg ttaacgacac
     3481 tattgactct cccctcattg agcttacgga ttaccttata tatatcaatg tcaacgacac
     3541 cgtcgagcct cgcaaattca ttaaatccgt cacagataaa tatgacgaaa aaacggatat
     3601 aagcaaggtt tcgataacct caacggttga cacaacaact gcaggtgtaa atcagatcaa
     3661 atatgaatac accgattcaa agggactgac aggccattca ttccttatag ttattgtcag
     3721 agagggataa cattatgaaa gaaaccacga cgactaaatc gaatcagatg aatatattca
     3781 tagtcctcag agatgtttgg cacaatgccc tgtttattct gatggctgcg attatcggat
     3841 tctccgccgt gaccatttac ggcagatatg tccgtgttcc cgagtatacg tcatcctcta
     3901 ctcttgttgt tgctgcaaag agcacaacct atgccaatgc ctatgcggcg ctttcgactg
     3961 catcctcaat ggcaggcgtt ctcaaggagg tctttgagag tgatatactc atgcaaaagg
     4021 tcgaggagag cgagggtaat ctgcccgccg ggatctccgt ttcggcaaat gtcatctccg
     4081 gaacgaacct tcttgtgctt gaggtcacgg ccaacgaccc gaaaaccgcc tatgttgtca
     4141 gcaattcaat actgaaaaat tataacggaa tttccgatta tcttttctca aatgccgttc
     4201 ttgaaacg
//
LOCUS       DAWBSW010000176         4191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3670_length_4191_cov_2.388781, whole genome shotgun sequence.
ACCESSION   DAWBSW010000176 DAWBSW010000000
VERSION     DAWBSW010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3670_length_4191_cov_2.388781"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    559..568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1168 a          891 c          920 g         1202 t
ORIGIN      
        1 catcgaccga ttatattgaa tttgttgcgg aaagagttga taaattcgga gcgattcgta
       61 caagaaaaat gttcggtgaa tacatggtct atctcaatga cagaccgatt ttttcagttt
      121 gtgacaacac cgtttttgtc aaaaaatttc ccgagctttc cgaaataatg aacggctcgg
      181 cgtgcggatt tccgtatgac ggcgccaaag aaagatatat tcttgacatt gaaaacgacg
      241 ggcttttgga aaaagtcgtt ccgctcctcg gcgaaattgt ccccctcccg aagccgaaga
      301 aaaagaagca agttttataa aatcaaatta agtattgcaa aaatttttgt ttttcattgt
      361 ttattggaat cgccgcagtc ggtgaaaccg taatgatgct tgcagacttc tcgcgaggca
      421 tatgtgagcg gacacttggc tcaaaattta ccgtaaggct cttgaacgct gctgccatga
      481 cggagaatac taaaaatgca aaatattttc ttttacagtt ttctcttatt gacaaaaaat
      541 ttttcataat atatactcnn nnnnnnnntc atccggagaa aaataaaatg ataaaaatcg
      601 aacatcttac aaaaaaatac ggcgatttca aggcggttga cgatttgtct ctgcacattg
      661 cgccgggcga aatatacggc tttatcggtc acaacggcgc cggtaaaaca acaacgctga
      721 aatccgtcgt cggtattctt caatttgata acggcgaaat ttatattgac ggaacatcca
      781 taaccgcaga tccgctcaaa tgcaaaaaag aaattgcata cattcccgat aatcccgata
      841 tttatgagtt tatgacagga attaagtatc tcaattttgt tgccgacatt ttcggtgttt
      901 ctgccgccga caggcaggaa aaaatcagaa aatatgccga gatatttgag ctgtcctctg
      961 accttgctca gccgatctca acgtattctc acggaatgaa gcaaaagctt gcaatcattt
     1021 ccgcctggat ccacagtcca aagcttatta taatggatga acctttcgtc ggtctcgacc
     1081 ctaaagctgc acaccttctc aaagggatga tgcgtgagat ttgcgacaac ggcggagcca
     1141 ttttcttttc aactcatgtt ctcgaggttg cggaaaagct ctgcgataaa atcgccatta
     1201 taaaagaggg caagcttgtc cgctcgggca caatggaaga ggtcaagggc gacgacagcc
     1261 ttgaagcggt atttcttgaa ctggaggaag aataatgctg aaacaacttc tcaaaaaaca
     1321 gttgcttgaa atgtttcaga acttttttgt tgacagaaag aaaaacaaag ggcgttcaaa
     1381 aacctcaagc ataatgatga tagtcggata ttttgctttt atctgtattt tgttcggtgc
     1441 actgttttca ttttttgcaa gcagcctgct ccccgttgtc gacaagggct atgactggat
     1501 gttcttctgc tttatgggaa ttatcgccgt tgcgatgggt gcgttcggaa gcgtgttcaa
     1561 tacatactcg ggcctttatc ttgccaagga caatgacatg ctgctttcaa tgccgattcc
     1621 cacaggttac ataatggcct cccgtctcct cggagtttat atcatgggca cactctattc
     1681 cgcccttgtt attatccccg cgattattgt ttatctctgc catgttttca gcgtaaaagc
     1741 gctgattggg tcgattctgt ttgttataat aatatcgttg atagttatga ttcttgcctg
     1801 tgcgctcggc tgggtcgttg caaaaatcaa cagcaagctt aaaaataaga gcattataac
     1861 agtgcttgtt tcgctcgcat ttctcgcgat ttattatgtt ttttatttta aggcaaatga
     1921 gatcatccgc agaattattt ccaatgcgga cgcatacgga accaaaataa aagtctcggc
     1981 atatccgctt tatattttcg gaaaagtcgg ttccggtgac cttctttccg cgtttaccgt
     2041 gctcgttatt gtcggtgctt tgtgtgcgct tacctattat ataattgcac acagctttat
     2101 aaaaatcgca acaacgtcgc aaaatgtttc aaagaaaaaa tatgttgaaa aaagagttaa
     2161 agcaaagagt gttgattcgg ctatgctcgg caaggagatt cagaggtttt tctcaagccc
     2221 gaattatatc cttaactgcg gcctcggtat tctgattctt atcggtgcag gtgcttttgt
     2281 gctgataaag ggtcagtggc ttgccgacat catatcaggg atgttcggaa gcgaaaacta
     2341 tatctgcgtt gcttctgcgg cgcttatttg tctgatcgct tcgatgaacg atatgtccgc
     2401 tccgtcggtt tcgcttgaag gaaaaaacat ttggattgca cagtcattgc cgataacctc
     2461 atggcaggca attaaagcaa aaagaagtat gcaccttctt ctcacaggta tacctgcttt
     2521 tgtttgcagt gcttgtacaa ttattgccct caagccgacg cccctcggcg ctttaatggt
     2581 gattgccgtg cctgcggttt ttatcctctt tttctcaatg ttcggacttg tgctcaatct
     2641 taaatatccg aaccttaact ggacaaatga aataacaccc attaagcaga gcatgagcgt
     2701 ttttgtttcc atgtttggag gctggatcta tacaatagtt ttcgcagttg cttattatcc
     2761 tttaagtgca cgaatttcag gcgagcttta tctcttgggg gtagccgcgt ttaccggtct
     2821 tttatgcctt gcgcttgaca cttggctcaa gaaaaagggt gcgaaaattt tcgccgagct
     2881 ttaagcaaaa ctaaacaata tgaaaggagt tgtctatacg gaaatcaaag taattgacgg
     2941 ctccttttcc gtttgtaagg ttaccgattt cagcgaggtt gacccgtcga aaaaattcaa
     3001 ttttatctcg gcaaccgacg aagagaattc gctgatctgt cttacggaaa atgttcccga
     3061 taatttcaca gagcgcattg acggctggcg ggctttaaaa attcagggag tgctcgattt
     3121 ttcgctcatc ggaattcttt caaaaattct cggtctgctt gccgaagcaa agataggaat
     3181 tgccgccgtt tcgacctaca acacagatta catcttcgtc aaagaaactg acctcgacaa
     3241 ggcagttgaa acacttgccg aggcgaatca taaaataatc cgtaaaggag gagccgaatg
     3301 aacaagaaat attttttaat cggaaacgct cacattgatc ccgtgtggca gtggtgcata
     3361 cccgagggtc tctcggttgt taaatcgacc tttcgctccg cactcgacag aatgatagaa
     3421 tacccgaatt acgttttcac gtctgcctgt gcctcatact ataaatggat aaaactcagc
     3481 gagcctgaga tgtttgaaga gatcaagcag agaattaaag agggtcgctg gaaatatgcg
     3541 ggcggaatgt gggttcagcc cgattgcaac attccctgcg gcgagtcctt tgcgaggcat
     3601 tttctctatt cacagagatt tttcaaggag aatttcggcg caataaccga gtcgggctac
     3661 aatgttgact cgttcggtca caatggcatg ctgcctcagc ttctcagaaa agcgggaatg
     3721 aaaaactatg tttatctccg tccgagccgt gatgcggaaa agaaggatct tcccgaaagc
     3781 tcgcttcact attggaacgg tcccgacgga agctccgtta cggcttttca tattcttgat
     3841 ggctacggcg atgacctcca tgacgagcga gttgcaagat atgaaaatca agatcactct
     3901 caaatgcttt tctatggtat cggaaatcac ggcggcggtc ctgcaaaaga gcatttaaag
     3961 caagcggaaa agctcatcga aaagggcaat tccgtttatt ccggtgttga cgaatttttt
     4021 gattatgtcc gtgaaaacga gaccgacgat atcccgaccg tcaacgagga tcttcagcat
     4081 catgcaagcg gctgctacag tgcaaattca aaaattaaaa agctcaaccg tgaggcggaa
     4141 aacgagctta tttatgcaga aaaaattgac gttctcgcct ctgttcttac a
//
LOCUS       DAWBSW010000177         4108 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3737_length_4108_cov_2.607205, whole genome shotgun sequence.
ACCESSION   DAWBSW010000177 DAWBSW010000000
VERSION     DAWBSW010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_4108_cov_2.607205"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2700..2709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1067 a          933 c         1006 g         1092 t
ORIGIN      
        1 aaaaagagct tgctctttca gtcggtttct tcggtgtcgg tacgcttttt gtaattcttg
       61 cctttttcag cctgtttttt aagtgcgcct attattccga aattgcaatg ataatttcgg
      121 ttatcgtttt tcttgtctgt gtaccgttta tttacggtaa ttataaggga ttgccgcaaa
      181 agacagccga aatcctcggc gctgttctgt cactgccttt tatcggcaat tggcttgcta
      241 ttgtcgtctt tgcaaaggtg agcgtaaatt acggaggctt agcagatttc tattatattt
      301 tttcacttgc cgcatttgtt ctgacggata taattctttt gctcagggct gcaggcgttt
      361 ttaagaagcc atatgctctt tcgtggctgt cattggcgct cggaattgca agcgtaattt
      421 ttactgtcgt atattttgca atatcgggtg ttgccaaaag cttagcggta gccataattg
      481 ccgcgcaggc gcttctgccc gaatacatgg ctttccacat acttatgacg gactacggaa
      541 aagaaaaaga tatgattaaa aacagataac agtgaggcta aaggcaatat cgcacaattg
      601 aggtgcgtaa attgcacagc atgacgaaaa tagattcaag caaggcatac gcctcgattt
      661 ttgcggcatt gccttgataa tataactcag tgaggagttt ttatatggca cttataacca
      721 aggcaatcaa gggaacgcag gatgttcttc cctccgagag ccataaaaac cagtttatcg
      781 aaagcacatt gctcaatatt gcaaaggatt tcggattcag agagatccgc acacctgttt
      841 ttgagcatac ggagcttttc acacgttcgg tcggagacac aacggacgtt gttcagaaag
      901 aaatgtacac atttaacgat aagggcggcc gttcgattac cctcagaccc gagggaacag
      961 caggcgcggc aagagctttc cttgagcacg ggcttcagaa cgagccaatg ccgcagaagg
     1021 tcagctatgt aacctcatgc taccgttatg aaaagccgca ggcaggcaga ctgcgtgaat
     1081 ttcaccagtt cggtgttgag tgctacggta cagcttctcc tgccgcagac gcagaggtta
     1141 tctcgctcgg caatgaaatt ttcggattcc tcggaattga cagaatcagt cttgagataa
     1201 attcaatcgg ttgtccgacc tgtcgcaaga agtatcagga agctctgaag gcatattttg
     1261 agtcccgtaa ggacgagctt tgcgacacct gccgtgaaag acttgaaaga aatccgatga
     1321 gaattctcga ctgcaaaagt ccgatttgtc atgaaatcgg gcaggacgca ccgataatcc
     1381 ttgactatct ctgtgacgag tgccacaacc actttgaaaa ggttaaaatg taccttgaag
     1441 cgatgaacat ttcatacacg gtcaatccgc acatcgtaag aggtcttgat tattatacac
     1501 gcacggtatt tgagtttgtg accgacgctc tcggtgcaca gagtgcagtt tgcgcaggcg
     1561 gtcgctatga cggacttgtt gaagaaatcg gcggtcctca tgttccggct ctcggatttg
     1621 cgctcggact tgaaagaatt cttctccttc ttgaggcgca aggaatcgca ctcccggctg
     1681 cggacgttcc tgagatttac atcggcagca tcggcgatgc ggctttgact aaggctgcgg
     1741 ctcttgcact tgagcttcgt cagtccggca tcagcgcaca gttcgacgtt gtcggccgtt
     1801 ccgtaaaggc tcagatgaaa tttgccgata agataggagc aagattcaca acggttatcg
     1861 gcgatgatga tattgccgca ggaattgcaa aagttaaaaa catggaaagc ggcgaaacaa
     1921 aggagatttc tcttgacagc ttttccgatg attttatgga atattcactt caatcggcag
     1981 ccatgaagct tcagggcact gccgctgagg atatggataa tatagattta agcaaaattt
     2041 taggaggctt aatataatgg actttatgac aggcttaaag cgtaccgact attgcggcga
     2101 cctcagaatc ggcgacgtcg gcagagaggt tgtcgttgca ggctgggtac agcgtcagcg
     2161 tgacctcggt gcacttattt ttatcgactt gcgtgacaga acaggtattg ttcagctcgc
     2221 attcggtgaa aacacagaca agaaaatctt cgacaaggct ttttcggcaa gaagcgaatt
     2281 tgttctcatg gcaaagggcg ttgtccgtga gcgttcttca aagaacatgg agataccgac
     2341 aggtgaaatt gaggttgatg tcaatgatct tcgtattctt ggcaagagcg agactccgcc
     2401 gtttgaaatc gttgagaatt gccagacttc cgagctgaca agacttaaat accgctatct
     2461 tgaccttcgt cgtcctgacc ttcagcgcaa cattatcatg cgccacaaga tttgtaagat
     2521 tacccgtgac tatttcgacg agaacggctt catcgagatt gaaaccccga ttcttatccg
     2581 ttccactccc gaaggagcaa gagatttcct tgttcccagc cgtattcaca acggttcctt
     2641 ctatgcgctt ccgcagtcgc ctcagcttta caagcagctt tcaatggttg caggctttgn
     2701 nnnnnnnnna cgctgcttcc gtgacgaaga tcttcgtgcc gaccgacagc ccgaatttac
     2761 acagatcgac cttgaaatgt cattcgttga catggaggac gttctcaata tcggcgaggg
     2821 atacatgaag cgagttttca aagaggctct cggcgttgat attcagcttc cgcttccgag
     2881 aattacttat aaagaggcca tggagcgctt cggttcggac aagcccgaca cacgttacgg
     2941 tatggagctt tatgatctta gcgatatcgt taaggactgc ggcttcggag tgttctccaa
     3001 tacagtcaag gacggcggct ctgtacgtgg catcacggca aagaatgctt tcaagtcact
     3061 ttcaagaaaa gagatagata agctcaccga ttcggttaag ggtaacggtg caaagggtct
     3121 tgcgtgggca agaatcgccg aggacggaac agtctcctcc tcctttgcaa agtttatcac
     3181 cgaggacgag ctcaaggcta ttcttgataa ggccggcgca gaagcaggcg acgttgttct
     3241 cataatcggc gacgttaaga attcaattgt tttcaacgct ctcggtgcac tccgtcagga
     3301 ggttgcaaag agacttgaca tcattccgga gggtcagtac aatctacttt ggatcacgga
     3361 gttcccgttc ttcgattggg acgaggacag caagacttgg gttgctatgc accatccgtt
     3421 tactgctcct atggacgaat gtcttgaata ccttgaaacc gataaggcaa aggtaagagc
     3481 aaaagcttac gaccttgttc tcaacggaat cgagctttcg tcgggttcaa tccgaattac
     3541 gaatcccgag cttcagagca gaattttcac ccttctcaac cttactcagg aggaggctta
     3601 tgcaaaattc ggttatctgc ttgacgcatt cagatacggc gcaccgccgc acggcggaat
     3661 gggtatcggt cttgaccgtc tcgttatgca gatgctcggc tgcgacagct tgagagatgt
     3721 tgttgcttac ccgaaggttc aaaacgcaag cgagccgatg acaggctgcc cggcagaggt
     3781 tgatgacctc cagcttgacg agctcggaat taagctcaaa acagaataaa aaactcgacc
     3841 gctttctgca atagagagcg gctgtttgta ttgtaggatt aagtaaaaag cactccgaaa
     3901 aatcaaattc tcggagtgct tttactgttt ttaattcatc agtcgatctc aacggagatt
     3961 ttctgatcgt ttctgttagc tgccgaacgc atgaccgtac ggatagcctt ggcaattctg
     4021 ccctgcttgc cgataacacg acccatatcg tcagctgcaa catgaagatg gtaaacaata
     4081 acgccgtctt cgtcaatatc gtcaactg
//
LOCUS       DAWBSW010000178         4045 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3800_length_4045_cov_2.551629, whole genome shotgun sequence.
ACCESSION   DAWBSW010000178 DAWBSW010000000
VERSION     DAWBSW010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_4045_cov_2.551629"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    340..349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3784..3804
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1159 a          821 c          990 g         1044 t
ORIGIN      
        1 cacgggttca ggtaaaagca ccctcgttca gcattttaac ggaattctca agccaaacag
       61 cggtaaggta gttgttgacg gcattgacat ttggtcggat aaaaaggcga caagagaagc
      121 tcgcttcaag gtcggtcttt gcttccagta tcccgaatat cagctctttg aggagaccgt
      181 gtataaagat atttcattcg gtccgaaaaa tatgaagctt cccgatgagg agatagcgaa
      241 aagagtcgtt tcggcggcgg catatgtaga acttaaaaag gaattgctcg aaaaatcacc
      301 gtttgacctt tcgggcggta aaaaaagacg agccgcaatn nnnnnnnnna atagcgggag
      361 ttatcgcaat gcagccgaag gttctcattc ttgacgagcc gactgccggt ctcgacccga
      421 taggcagaga cacaatactt gatatgataa agaattaccg tgaaaaaacg ggaagcaccg
      481 ttctgcttgt ttcacacagc atggaggaca ttgcgaagat agcaaccaag gttgccgtca
      541 tgaacaacgc aaggcttgaa atgtacggaa cggttgacga ggtctattct cacgccgaaa
      601 gactcagaga aataggactt aacattccgc agatttcaaa agtatttgaa atactcaaaa
      661 acaacggaca tgatttcggc tcggtctata ccgttgaccg tgccgttgag gttttgacga
      721 ggtaaaccgc tattattttc cctgcacagg aggtgcaaac ggtgttaaaa gacattacta
      781 taggtcagta ttacaacgtt gaatcaccga ttcatcgcat ggacgcaaga atgaaaatca
      841 tactgacggt tttgttcatt gtttcgattt ttatgtgcaa aagctttgag gcgctcggtc
      901 ttatggtgct tttcacattg acggttaata taatttcaaa ggttcccgtg aagatggtcg
      961 caaaaagtct caagccgatc gtcgttatag ttctgttcac gtcgatattg aacattttct
     1021 atattagagg cggcacggag cttgtgaact ggcattttat ccacatcacg acaaacggac
     1081 tttttaccgc tctgtttatg gcgataagaa ttgtctgtct tgttgttatc agctcacttt
     1141 tgacctacac gacaacgccg accgttctga cggattccct tgaaaagctt tttgctccgc
     1201 tcaagctcat aaaagtgccc gtgtcaacgc tcgcaatgat gatgacgctg gctctgcgct
     1261 ttatcccgac tctcattgac gagatagaca gaatcacaaa cgctcagaag gcgaggggag
     1321 ccgaccttga taacggcagc tttatgcaaa gggtcaaggc acttgtgcct atcattgttc
     1381 ctctttttgt ctcttcgata agacgtgcat acgagcttgc cgacgcaatg gactgccgct
     1441 gctatacggg cggtcccgga agaacgagaa tgaaacaaat gaagctcaga gcaacggact
     1501 ttgtgtggct cggcgcagca ctcgtttatt ttgcgggaat tattgtactg aattattatt
     1561 tcaaggcggt gcttgttaaa tgagaaacct tgcgcttcgc ctgatgtatg acggcacgga
     1621 ctttcacgga tggcaggttc agccgaacgg aataaccgtc caggaatgct tgcaggacgc
     1681 cgttgaaaaa attctcggcg tgcgtgaaag cataacgggc tgcagtcgta ctgatgccgg
     1741 cgttcatgcg aacgattttt gctgtacgct gaggacggag agctccattg aatgctaccg
     1801 tttgaccggt gcgctcaatg ctgtattgcc cgactgtgtg gctgtcaaat cctgtaagga
     1861 agtgccgctt gattttcacc cgaggtacga ttgcaaaagc aagagatatc tttacaggat
     1921 atggaactcg caggcgaaaa atccgtttct caagaactat gcatggcatt ataaatcaaa
     1981 gcttgacgcc gatattttaa atgaacaggc aaagcaattc ataggaacgc acgattttgc
     2041 cggtttttgc tcctcgggca gcagcgttga ggacacggtt cgcacgattt atgactgcgg
     2101 cgttgtaagg aacggcgatg aggtcgattt ttacgttgag ggcaacggtt ttctttacaa
     2161 catggtgagg ataatggtcg gctcgttaat agaaatttca gaaaaaagaa ttgaaaaaga
     2221 cacactgaca gctataatag aaagtaggga cagagaaaag gcgggcagaa ccgcccctgc
     2281 gtgcggactt tttcttgatt atgtaagcta tggggaggca gaagtttgaa aagctcggga
     2341 aaatcaaaaa aattcaagct tagaataacg gatcgttcaa agaaaattct gacaatcgtt
     2401 ttagctgttg ccgttgtaat tcttattgtt atgacggcag ctcagaagct cggtaatatg
     2461 actgtatcga cctttacggc cgatgttaaa acttatttta tgagtctcgg ctcgggcgac
     2521 ggatatccct atgaaataag tgccggcaag gttaagaata tcacggtaaa caattcaaat
     2581 ctaaatcttt tgcttgacga taaaacaatt tcgttgacct cctcggcaaa ggagattatg
     2641 ccgaaaagcc acacatattc aaacccggtg atgaaaagca aaggctcaaa aatgattgtt
     2701 tttgaccttg attcgggcaa attcagagtt cagcgaggct cggatacagt aatcgaaaag
     2761 tcgcttgata aaaatatcat ggcggcggcg ataggctcaa agggcaatta tgcggttgcg
     2821 acctacggca aggatgccca gtccgtgctg acggtttaca acaagaacgg caaagagaaa
     2881 ttcgcatgga atttcagcga tgagaggatc acggatatcg acctttcgcc gaacggaaaa
     2941 tatgccgcag ttgccgcaat taaggctttg aacggtgaga tatcttcaaa gctttatgtt
     3001 ttcaacctca gcaaggcgga cgaatatacc gcatgctttg attacggcgg aacaacgctt
     3061 ttgaaggtga attatatcaa gggcaacaac attgttgccg tgggcgataa ccttaaatcg
     3121 tacatcaaaa acaatacgaa caggcaggac gacctgaagt tcaattccga taagcttcac
     3181 aattattgcg tgagtcagga cggctcgtcg gttcttgctc tttcaaaata cggcagctca
     3241 tcgctttcaa cgctcagttt ttacaacagc agcaacaagg agaggtttac cgttgatttc
     3301 gataaggaaa taagaagcgt tgatacggac ggaaaatacg tcgctgttct ttttgacagc
     3361 gaggtcaaaa cgtttaacaa gcgaggcaag caggtcggaa cgatttactt cacgggcgag
     3421 cctaaaagag tgacggttga cggcggcagg acatatattc tgaccggcgt gaatataaaa
     3481 agttataaaa caagaggaac cacggacgaa aggcaggtaa gcaaataatg caggagcata
     3541 tagttgatat aatccttgcg gcagtaattg tgctgatgac ggtaagatat taccgacagg
     3601 gacttgttca gacgatattt aaattcggtt cgtttatcgt tgcgcttttg gcggcgagaa
     3661 ttttctgcgg tgccgtgacg gactggatat acagcaacac aagtcttttc ggcagcacgg
     3721 agagatacct tgcaaagctt attgtatttg tgcttattta catagcggtc agactcttgt
     3781 tcannnnnnn nnnnnnnnnn nnnngataat tcatcttttg gactcactga aaaagctgcc
     3841 gatacttaaa aaggcaaaca aatttctggg cgctcttctc ggcttcggct gcggtgttat
     3901 ggctgtaata gttttgagcg tgggcttgca aatatcctcc catgtggtgt ataatgcgaa
     3961 atatgtaaat gcgatagatt cttcggtcat cgtgcaggcg gttatttcaa acgataagat
     4021 tgccgagaat atcaaaactt tcaat
//
LOCUS       DAWBSW010000179         4016 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3823_length_4016_cov_2.432719, whole genome shotgun sequence.
ACCESSION   DAWBSW010000179 DAWBSW010000000
VERSION     DAWBSW010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_4016_cov_2.432719"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1067 a          821 c          970 g         1158 t
ORIGIN      
        1 agattccgtc gtccggactg ctgatttaca gggtcaacaa gtctgttgtt aatcagacga
       61 atgcctgggg cgacgattat ctttacgttt accgtcccgg cgagacaagc acaacggcga
      121 gcgcaggtga tttgttcaaa tctgctcttg acccgaacag taacaggaca agcttcggcg
      181 ttgcggattt cgacgcatcg ctcactgacg gaacgatttt ctattccaac ggtaaaaaca
      241 gcggtatagt tatttcggac gtaaagtata acagtgattc atctcaaatc agctttcatg
      301 ttgagttccc ggactactcc tcactcggtt tgtggaacgg tatttcaaac agcattgcaa
      361 tggaggcaac cggaataaaa gttgataccg acagtatggg caatgtctat tcatgtataa
      421 tgggtcgtga ggactggaac tttgtgaaca aggtgcttaa gtacgacggc gcatcatgga
      481 ccgcactcgg ttcgtctttt tcaaatgtta acagcatgac gcttaaggtt tataacgatg
      541 ttccttatgt tctctacctc aattcaagcg gcagacccgt tctcgcaaag tacgcaggca
      601 actcgtggca gaccgtttat acggataact cggtcagcta tccgaacgat ttgcagcttt
      661 ttatgggcga ttcggggttc tactgcgcat ggacggttga cggtacaagg ctgagtataa
      721 agaaaattac ttcgaacggc gtgacaaatg tcaattcatc gctgactgcc gattattttg
      781 caaatccgag gctcagcacg gtcggcaatt atatttatgt gacctattgt aatttctcct
      841 tcggcggagg aaagcagtat acgcaggtca agagatataa tctgaccgag ggacagtggg
      901 aaagcattca ggttccgaat ccgcttgtct catccaatct tcacagaagc atcggatata
      961 acggtgaata ctggatgatt gcggcaacgt cgggcagtaa accgataata gtcaaggtcg
     1021 acggcaattc aaatgtcact caatatgagg ttcctaccac gataacaaat attcttgagg
     1081 cttcaatgga tatttccgaa aacggtacgg tttgtgtttc actcattgcc tcgggcgagg
     1141 aaagtcagat actttatctt gacggcggcg agtggaagca gctcggcggt tcgccctgct
     1201 caagctgtca gtcggctgat atgacgattt acaaaaacag ggtttatgta ggctcggttc
     1261 tcacgggtac gggaggagtc agtctaactt ataaggattt gcccgagaag gaattgcccg
     1321 agcttatctc aatcgaatca cagacggttt cggtaactga cggttatata atcggtctgc
     1381 cgcagagagc cgcaaacctc aagctttatc ttgaagcaac gaatggcggc tattttaagt
     1441 atgataaggt ttgcacgggc ggacaggttt tgctttacac tgccgacggc gtgcttgtaa
     1501 aaagatatac ggttgtaatt aaaggcgacg tcaacggtga cggcattgcc gacggatgtg
     1561 atgccgttct tataaatgcc tcggcggcag gaatgcttac gtttgagggt ggcttcaaaa
     1621 aggcggcgga taccgacggc gacggaagca ttacggaaaa ggataacgaa tatccgataa
     1681 aaagcggatt gaatttataa aaaatatcaa gaggggaggc ggcgcacttg cggggtaaaa
     1741 aggcactgct gaattctgcg gcatcgctga tgcttgagtt tgtgacggta atatgcggct
     1801 ttatcgtgcc gagacttatt atcggaacat acggctccga ggtcaatgcg ctgacttctt
     1861 cgatatctca gtttctcagc tacattgccc ttgttgaggc aggtgtcggc ggagttatca
     1921 gagcggcact ttataagccg cttgccgaaa aaaacaatgc tgcgatgagc agcataataa
     1981 aggcgacgga aaactttttt cgtaagatag cttatatttt cgtcggctac atggtcgttc
     2041 ttgcggcaat tttccccgtg cttgtcaaca agaattttga ctgggcattt accgcctcgc
     2101 tggttataat aataggattg agcacctttg cacagtatta tttcggcatg acatataccg
     2161 tgctcgttca ggcggatcag aggagatatg tcacctcgtt tttgcatata ggcacaatta
     2221 cgctcaatgc gataatggtc gtgattttta taaggctcgg cgcttcaatt caaatcttga
     2281 agctcgcaac atcgcttatc tatgtcctgc gtccgcttgc tttgaatgtt tacgtcaaga
     2341 aaaaatataa tattgaccgc aaagcaaagc ccgataacga agccgtcgca cagcgctggg
     2401 acggtctcgg acatcacata gcatatttcg tcaatctgaa taccgacgtt gtaattctta
     2461 cgattttctc aaaaatatca tcgtctcttg ctctcgccga ggtgtcggtt tatacggttt
     2521 attattccgt tgtcaacggc attatgaagg ttgcgtccag tctctcggca ggcgttgagg
     2581 cagctctcgg agacatgatt gccaagaagg aatacgaaaa tcttaacaga aagttcaatc
     2641 tttatgagtt tgtgtcgttc aatcttgtaa cggtgctttt ttcctgcgcc ggacttttga
     2701 tagtgccgtt tatgagcgtc tatatgaagg gcgttacgga tgcaaattat ataagacctc
     2761 ttttcggata tattctcgtg cttgcgaatg cgatgtattc gattagaatt ccttatcaca
     2821 gtgtggttta tgccgcaggg cattataaac agacccgtaa cggtgcattt gccgaagcgg
     2881 gaataaacat agccgtttcc gcaattcttg tttattttta cggaattgtc ggtgtggcgg
     2941 tcggcacgct tgttgcgatg acgttcagaa cggttcagta tgtttattat ctttcaaaga
     3001 atattctgaa cagaagtgtg aagtttttct tcaagcgaat ggcgatttcg attctgacgg
     3061 ttgttatttc ggcaggcgtt gtgcatttta ttccgtcgta cagaactaag agctatctcg
     3121 gctgggtgct atatgcatgc gcagtcggaa ttatcgtttt aacggtaacc tgcggaattg
     3181 atttaatatt ctgccgcaag gattttatcg gagcggttaa ggttataaaa aatgcaattt
     3241 ttgcaaaaat aaaaaagaaa aagtaattga ttggagaatg aatcatggca gttaagaata
     3301 aggaaaaaat ttataagctc acacttaccg cagtgcttac ggcgttgatt tttgtaatgg
     3361 cattcacacc gctcggctat ctgaaaatcg gaacagtttc cataaccttt cttatgattc
     3421 ccgttgttat cggcgcaatt gtcggcggac cctcggtcgg aacggtgctc ggactgatgt
     3481 tcggcgcaac gagctttatc cagtgctttg gtatggacac tttcggaaca actcttttcg
     3541 gaataaatcc tgtttatacc gcaattatgt gctttgtacc gagaattctg atgggtcttt
     3601 tctgcggact gatttataag gctttcggca aaaagaatta cattaagacc ggtacggctt
     3661 taacatcgtt ctgcggcgga tttattaaca ctgttctttt tgtcggagca ttgcttctcc
     3721 ttttctacaa ctccgactat attcagagct tcggcaacag cgtgattgcc gttatctcgg
     3781 tgcttataac attcaatgcc gttatcgagt ggatcgcctg cacggtcatc ggctcggcgg
     3841 tctcaatcgc actgaacaag gcgttgaaaa aaagatttta attgaacggc ttttacttga
     3901 tttcttaaga tttgcttaat atttaatctt taatatagac atttagaaag tttaatggta
     3961 aaataaaaga aggtcaaggg aaaagaaaaa gctaaataat atgcatatca atatcc
//
LOCUS       DAWBSW010000180         4012 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3832_length_4012_cov_2.616881, whole genome shotgun sequence.
ACCESSION   DAWBSW010000180 DAWBSW010000000
VERSION     DAWBSW010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3832_length_4012_cov_2.616881"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    467..476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          948 a         1056 c          891 g         1107 t
ORIGIN      
        1 tcgaaagcga agcctacgtc gcagccatat tccttaacat atctctgaag tccgtcaaga
       61 tgagtcgaac cgcactcacg gttgatattg atgccgttcg gcattgacga cttgatgtgg
      121 cagtcaaccc caagctcggt aaaaagatcg ggagccgtta cgcttgccgc accgtttgcg
      181 cagtcaagag caaccttgta gcccgaaaaa tcaatatcct caaccgtgga aataagatga
      241 cgtgtgtaat caaacgtcgg catctcggag cttctgatac gtccgatatc agcgcctatc
      301 ttaacaggcg gctgagttac gccgtcaagg ataatcgctt caattttctc ctccagttca
      361 tcgggaagct tatagccgtc cgcctgaaag attttgatgc cgttgtattc gcacgggtta
      421 tgcgaagcgg tgatcatgac cgctgcatca tactcatatt ttttaannnn nnnnnngagc
      481 tgcataacat ctgcaccgac cgagcaaagg cctgcagtga tcgcagagca aagcatattt
      541 gatgaaattc ttgtgtccat tccgatcata actctcggac gctttttgtt ctcgtcaata
      601 agaaccatcg cagccgcacg gccgatctgc atggcaagct cgcatgtaag ctccgagttt
      661 gcaactcctc ttgcaccgtc tgttccgaat aatctgccca taaaatgacc tccgaattat
      721 agttcaaatt tttgctgttc catcagcgga accgggttgt tattagctcc gttctgtgca
      781 ggcatcggca tacccaaatg tgcatagccg gcaggcgtaa ccatacgccc tctcggagtt
      841 ctcgaaagaa atccgatctg catcagatac ggttcataga catcttctat tgtgatcgct
      901 tcctcgccta tcgcagcggc gatcgtttca agtccgacgg gacctccgtt atagttcttt
      961 atcattgccg taagaagaag tcggtcgatt gaatcgagac cgagcgggtc aacctcaagg
     1021 cgattgagcg caaggtcggc attttcgtcg gtaataacgc cgttgccgag cacctgtgca
     1081 aagtcacgga cacgtttcaa aaatctgttt gcgattctcg gtgttcctct tgaacgggag
     1141 gctatctgat aagctccctc gtcggtaatt tctatattaa gtattcctgc gcttctttta
     1201 acgatactgc tgagatcctc gggcgaatac agttcaagtc ttgcaataac gccgaaacgg
     1261 tcacgaagcg gagcactgag ctgacctgcc cttgttgttg cgccgacaag ggtgaatctc
     1321 ggcagatcta ttcttatgct tcttgccgac ggtccctgac cgatgattat atcaagcgca
     1381 ttgtcctcca ttgcgggata gagaacctcc tctacactcc tgttcattct gtgaatttca
     1441 tcaatgaaaa gcacatcgcc ctcgctgaga tttgtaagca atgcagccag atccttcggc
     1501 ttttcgattg caggacccga ggtcacacgg aagttaacgc ccatctcgtt ggcaattata
     1561 cctgcaagag ttgtctttcc gagacccgga gggccgtaaa gaagcacatg gtcaagcggt
     1621 tcgtttctct tttttgccgc ttctatataa attgaaagat tttccttaac cttagtctgt
     1681 ccgatatagt cgctcatcgt cctcggacga agctgaaact ccacatcgga atcgttatcc
     1741 atatactcgg gagcgactat tcggttttca aagtccatat cctccatgtc gttccctcct
     1801 tatatcagtg atttaagtgc ctgtcttata agctcctcaa tcggcagggt gctgtcaagc
     1861 ttaccgagtg ccgccgcagc ttccgacctg ctgtagccga gctgaacgag agcttctatc
     1921 gcctcgccca cattctcggc acctgccgtc atctgaaccg ctccgacaag ctcggactgc
     1981 tcctcggtaa gttctgtgct gaacttattt ttgagctcaa gaataattct gttggctatc
     2041 ttcgctccga cgccctgtgc ctttgttata gcctttgcgt cgcccgaagc aatgctcaaa
     2101 gcaagcttat cgggagtcag aacggagagt atcgcaagcg ccgcctttgg acctacaccc
     2161 gaaacggtga taagctgctt aaaggaatca agctcatttg gcgttgtaaa tccgtaaagc
     2221 tccattgcgt cctcacgaac gttaaggtag gtgtaaaccg tcgtttccgt gttgagtgca
     2281 acatttttca gtgtattcat ggtggaaaag cagaaaaacg caattccgcc gcactgaagc
     2341 gccagtccgt tcctgtcaag ggcaactacc ttgcctgtaa gagaatatat catcgtttcc
     2401 cgcctttcgg taaataaaat attttttgtc tcccataaaa gggagccaat gataagtctc
     2461 cgctaacatt tatcagtctt tcccttgaga gaaagccttt ttaagccata gatcaatgat
     2521 catcgactcc tgcgaattca tcttcgccgc gcattatcaa taatacttct tcagctttcc
     2581 gacgagcgaa ccgctggtgt gtgcatggca aatagccatt gcaagggcgt ccgccgtgtc
     2641 gtcgggcttc ggtatttcgt caaatttcag cataattcgc atcatttcct gcacttgctt
     2701 tttctcggct ctgccgtagc cgacaacgct ctgcttcacc tgaagcggcg tgtattcggc
     2761 gatttcaagc ccgtggagct gcgccgtcag cacaataact cccctcgcct gcgcaacgtc
     2821 aatgactgtc tttgcatttg tgttgtagaa aagcttttcg atcgccatga catctggttt
     2881 atattgggtc attatcaaat caagctcacg gtaaatgatc tccagtcgct cgttaaaagg
     2941 cgttcctgcc ttggttgtta ttgcgccgta gccgagcacc ttgaaatgat tgttactgta
     3001 ttccagcacg ccgtaaccga ttatcgcata gccgggatca attcctaaaa taatcattat
     3061 ttcttttcgt ctctttcacg gattaccatt ttcgtcggcg taccctcaag tccgaaaacc
     3121 tcacgaatct gattgtcgat ataccgctga tagctgtaat gaaaaagatc cttgcggttg
     3181 acaaagcaga caaatgtcgg cggtcttgtg gaagcctgag taatataata tattttaagc
     3241 cttctgcctt tatcagtcgg cggctgaact ctcgccgtcg cttcggcaag aatatcgttg
     3301 agctttcctg tcgaaattct cattgcattc tgagtgtcaa caaacttgat aagctcaaag
     3361 agtcggtcaa gccgctgacc cgtctttgcc gaaataaaga taatcggtgc ataagacata
     3421 aacgaaaaat cgttcatcag ctttttacgg tagctgtcca tggtcttgcc gtccttctcg
     3481 actgcatccc atttatttac ggcgataata cacgccttgc cgaggtcgtg ggcaattccg
     3541 gcaaccttag aatcctgctc cgtgaagccc tcctgcgcgt caatcataat aacacacacg
     3601 gttgctcttt caacagccat tctcgcacgg agaacgctgt atttttcaat tgcttcctca
     3661 accttgctct tgcggcggat acctgccgtg tcaatgaaaa tgaacttgcc gaaccggttt
     3721 tcaatcaacg tgtcggtagc gtccctcgtt gtgcctgcga tatttgaaac gatgactcgc
     3781 tcctcgcctg caatggcatt gataagtgag gatttgccga cattcggctt gccgataaca
     3841 gcaacacgga ttctgtcgtc gtcctcgtcc tcatcttcct cctcggggaa atatttgata
     3901 acctcatcga gcagatcgcc cgtgccatgg ccgtgaacag ccgaaactct tatcggctcg
     3961 ccgaggcaga ggttgtagaa ctcatagaat tccatcggcg gatcgcctat tg
//
LOCUS       DAWBSW010000181         3965 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3868_length_3965_cov_2.435550, whole genome shotgun sequence.
ACCESSION   DAWBSW010000181 DAWBSW010000000
VERSION     DAWBSW010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_3965_cov_2.435550"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1772..1781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1131 a          835 c          923 g         1066 t
ORIGIN      
        1 accgttctcc acccggtgaa cgacagcaaa aagagtaaaa acaaggcttc attctttcac
       61 ttcccgaaaa gttttctata ttataactct ttttcgtctt ttttgcaatt ataacggcat
      121 aaaaaacaaa ataaggcgaa cactattttt gcaaacgata aaagaaccgt tcatcggtca
      181 tcaaagagga gaataatata tgaaagcaac cggaattgtt agacgaatcg acgacctcgg
      241 acgtgtggtt attccgaaag agattcgtcg cacaatgcat atcaaggagg gagctccgct
      301 ggaaattttt aaggacacgg agggcggcgt tatatttaaa aaatactcgc cgataagtga
      361 gatttccgat gtgacggagc atttcgccga tgtgctttac agaagcgtta ccactccggt
      421 tttaatctgt gacagagacc atgttatttc atgtgccggt gtttcaaaaa aggattttct
      481 tgagaagcga atcagtgctg aattggaaag cgtaatggag tcaagacagc tctacaccgg
      541 tgatagagat aagcagcttt ttccgattga gggaatcgac tatggggctt ctattgcgat
      601 gccgataatc ggtaacggag acattctcgg agcagtggtt tttctttgga gcgacggctc
      661 caaaatttcc ccgacggaaa caaagctggc tcaggtagca gcgtcgttta tcggcaagca
      721 aatggagcaa taactaaggc gatatcgcac gcttagattg tgcggtattg tccacgaaaa
      781 cgtgtcttaa ccgtttggtt tcaggcacgt tttaattttt ttatgatgaa aaatattaag
      841 aaaaaaggtc aattcttaag aactgattaa gatttaatta agaaccgctt aagattgaat
      901 taagtctatg gacaagtgat tttcaggtga tataatatcg cttgtgagca tgaaacggct
      961 tgttataaca tgctgtttca tgctgatgta aattggaaag agatacacaa acgtcgggga
     1021 aagaaaaata aaaatgaaaa agggtcggca gcgccgaaag agaaagagtc ggtaaagcag
     1081 gttttgcgat aaaaaactgc ttgtcgaggt agggcgatgc tttgcgaggg ggaatcaggg
     1141 gaaaagattc aaaaacagca aagcataaag tccctatctt caagaagaaa aaatcagccg
     1201 cataaaacgt tgtgtttaat gcggctgtga tgatttttaa aaggtcaggt tgaaatgaag
     1261 aatatcgttg caccgaggaa catctgagcg tagaaatatg tccagatatt cacctgcctg
     1321 agtgctccgt gcttttcaac gtcaggcttg aaattgagaa tcgaaagcgt atagtctgaa
     1381 agcatgaaaa ggaacgcgcc gagcataagc attatgcctg taaatatcgg agattcaacc
     1441 aggcttccgg atttcattat cattatagca agcgaaaaag ccttaacaac cattgctgct
     1501 atcgttgccg tgtagagaat acagggaatg agcagcttgc caagctcaat tttgacaatg
     1561 aatattgccg aaagaatggc ggcaacgacc agaattatgt atattatcaa ttccggcgca
     1621 gtaacaaggg gtgtgtcggg gaataaaacc ttgaaagcct tacaatatgc aatcgtgtag
     1681 aaaacatgag cgacaaggaa cgatgaaaga cccgcaacaa aaaatcccga gccggggcgg
     1741 acatggagca gataatctcc gagccatgaa annnnnnnnn nattaggttg ctccgcttta
     1801 agaaagaatg gaataacaat tgccaatatc acaagcagta aagccaaaaa aacaaaatac
     1861 attctatcat ccatattggt gtcacctcga ataaaagatt atttattttt attctctcag
     1921 gggtgttttt tatttaattc ttccgagtat gggtgcgtca ataattccgg gaagttggtc
     1981 aaaggcaacc agtccgaatt tatggagtat cggcgggaag aacgaatcaa cgtccagcgc
     2041 caacgattct gccattgcac tcatcggcat ctgacttgac ggacggagag taacgatatg
     2101 cccgttgaaa ttgagcttga tttttctcat acatattcca ccgacgggac gcatatgtac
     2161 ttcgagcgtg ttttcgtcaa gccatgccgc cgtcgaaaat gccgtaaagt tcattccggc
     2221 aagttttatc gggcttgttc gtgccttgcc gtccattccg cagagaatgg tgttatgctc
     2281 gtcgccctcg tcccatgaca ttgtgcaagt gtcaccgtca aaacgaaaaa caatgttatc
     2341 aacatttccg gctctgtctc cggacatata aactatcggg atcgggagca tactgaacgg
     2401 gaatcccgcc gcattaagta tcggatttat ctgcatggca attgttttgc cgtcaagaga
     2461 tttctcaaat ttactgtgct ctcttggctc aagatcatcg ggcagagcac cgagctcggg
     2521 cttgtttttc ggcgtcggtt cgctgtcgtt ttcaatgatg aggttgttaa agtggcgcca
     2581 gatgcagtct cttgtttttt gttcgttaat ttcaccggcc gtcatgacgt aaacaatgtc
     2641 gtcgtcattt gagacgattc cgaactgaga aaacattccg tcggccctgt agccgttaat
     2701 tccgccacag cgccagaagc aatatccgta gccgcactgg ctgtcaataa cattggtgaa
     2761 cggagaattg tttgcgtgct gtttctgcgc aagataagtc cattcctcgg ggattacctg
     2821 cttgccgtta aacataccgt gctgctgata acagaacata aattttgcaa ggtcttctgt
     2881 cttgaggtaa agaccccagc cgcctgcctc aattccgtca atgtcgtgct cccagaacgg
     2941 gacatcaatt ccaagcggtt caaaaagtct cggagttaaa aattccacaa cagacattcc
     3001 cgttatcctt gtaagcatcg cacagagcat gtactgattt tcgcttatgt actgccagtg
     3061 accgtctccg ggcgaaaact tccaggcaga atcaaaatag tccttgatcc aatgattttt
     3121 cgccttatcc gagaaaacac tgacgttttt accggattgc atggacagca agtggtgaac
     3181 attcagcttt tcaagatttt cgtccttaat tttcggcctg tattcgggga aaatgtcaat
     3241 gagcttggtt tcgagcgtca tcaagccttc cgcaatggca aatccgaccg ccgttgaagt
     3301 gaagctcttg ctgacggaat acatcatgtg gggatattcc ggcgaataag gagccgccca
     3361 tgtttcgtag gcaaccttgc cttccctcaa aatcattata ctgtgaactt caatgttttg
     3421 tgcacggaaa tcctctataa gttcggatat tgttttggac gaaattccga cctgctcggg
     3481 ataatccgcg cgtggaagac ggattttttc tttgatttcc atgtatgttc cctcctattt
     3541 taaccgcctg tcaagcggtt gattcccttt tgcttctcaa aaagagagtt ccgcccgtga
     3601 atgccgccgc acaaagaaca acggcgataa ttctgccggg agtaaatctg aatttaagcg
     3661 caacattttt cttcgcaccg accttcagcg agtcatgagc cgattgcagt gcggagcgag
     3721 cgtccgtaag ctcagaaaag gaagatctac cgtcaagcgt cttttttgcc gccgaaagag
     3781 cggaggaaaa ggctttttcg gtctcgcttg tgtagggcga aagatccgtg ttggcataaa
     3841 tatcaatatt tttttcgaga tcggttgccg ccgtctcggt ttttcttata tattctgtca
     3901 gctcggcagg aaaatcggtt tcgataacgc tgtagcctcg ggaaataagg tcatcccagc
     3961 cggtt
//
LOCUS       DAWBSW010000182         3956 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3878_length_3956_cov_2.238913, whole genome shotgun sequence.
ACCESSION   DAWBSW010000182 DAWBSW010000000
VERSION     DAWBSW010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_3956_cov_2.238913"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1448..1457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3039..3048
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1158 a          828 c          783 g         1167 t
ORIGIN      
        1 ccatgtatca agaccgcatc ttgcatcttc gagaatcata tcaaagaatt tccccagggg
       61 tccctcccct tcttttttgc cgacaacaga ggcatatgaa ataacattcg gtttattttc
      121 aaaatcaata attctgccgc tcatttattt atcacctttt caactataaa ataaatcaaa
      181 ccgtaaagtg aagcggctgt tgttccgtaa agaataacgg gtcctgcgac cttgaatata
      241 cttgcgcctg tacccattat aaatccctcg cttttgaact ccatagcggg agaaacgacg
      301 gaatttgcaa agccggtgat aggaacaatc gtacctgctc cggcatgctt tgctattttg
      361 tcaaaaacac ctattccggt cagaattgcc gtgataataa tcaatgctat tgaaaccaga
      421 gaacggctct catcaaggct cattccgctt tttctgaaaa tgtaaaacaa aagctgaccg
      481 aagcagcaaa ttgcaccgcc gattaaaaat gcttttatac agtccaagac tataggagaa
      541 ttcggtgaag ccttttttac catttcatca tattgtttat tcgatattct cattatcaac
      601 gctccaaaaa taatgttcga taataatatt tgcaaataag cggaaaaaat acaaaaaata
      661 aggacgaccg aagccgtcct ttaaatttat tttgccttaa tcaagcgccg actgccttgt
      721 aaagaatttg caaagcgtca tagctgttaa cctttccgtt agagtccata tctcctctgt
      781 taaaggcgtc ctttgaaagt gttgtgattc ctacaacgtg ctgaagaaca agaagtgcat
      841 ctgccgaatt tatcttgtta tcgtagttaa cgtcaccctt gattggcttg gtaacaatat
      901 tgatcgtgta ggtgtctctt gccttcatat tgctcttgaa gtatacctca ataacattgc
      961 ttccgagagc atattctttt ttgggtgaag catagatatt ctgagttacc tgaacgcccg
     1021 aaacagtttc tttataggcc tcagttcttg ttgaaccgtc ggaatacgtt acctttacaa
     1081 caagacctct catgtcgatc attcctctgt cggcataacg ggtataatat ccgccgtgat
     1141 acttgaaggt aatcagaccc tccagcggaa caaaggttcc gagtccgccg ccctcgggca
     1201 tatcgtaata tccgtagtcc cagtctgttc cctgaacaaa ttcagtcttg tttgggtatg
     1261 tcaaaatttt aactgccgta attttcggag atgttgctgc ggagacgatc gggataagtc
     1321 ccgtacatgt aagtgctaca attattgcca taatacatgc aaataaacga taaagctttt
     1381 tcataaatta ttacctccct taatattcta tttaattata aacgtaaaat atgaatttgt
     1441 caataatnnn nnnnnnntat acgccttgca aatcaaaatc ggggataaat tcttccgatg
     1501 aggaatcaag ctcttgaata taaacattct caaagccgag catttcagcc tttgaaagaa
     1561 ccgtttcgta ttctctgtga ttgatttttc tgttaattgt aggatattcg gatgcccttg
     1621 cgcatggcat atactgtccc atgaggctga ttatagtatt gcttgaaaga ttgccgttga
     1681 tccattcgag cgcttttata ccctgactta tatttccggg taaaacaaga tgacggatta
     1741 tcaatccttt ttttaaaatc ccctcttcaa aaatatcctc gggctgttga cggcgcattt
     1801 caagcacggc ttttgaagca acttcaaaat agttttcagc tcggctgtat ttttttgaaa
     1861 cagtcgggct tacatatttt atatccgtca gaaaaacatc aacgaggcct tcgagctttt
     1921 ttagagtatc aacactgtca tatccgcctg tgttataaac aacgggaatt gaaggcttgt
     1981 attttgagag tgtaacggaa agctgttcgg catagtgtgt gggacttaca agatttatat
     2041 tatttgctcc ctgcgcttca agctctttaa aaatctcaat gagtcttttc tcggagattt
     2101 cttttccgaa gcagccatca ctgatgctgc tgttctggca atagacacat ttcagcggac
     2161 accctgagaa aaaaacagtt ccgcttcctt ttttgccgct tatgcagggc tcctcccaat
     2221 aatgcaatgc cgctcttgcg accttgtagt tttgccctac gccgcagatt ccgacagcta
     2281 cgcttctgtc aacagagcat tttcgcggac actgattgca aatatacatt aaaaacacct
     2341 cgttttgatt acaaatatta tatcataaaa ttttttcttg tctatagttg gcagttgtga
     2401 tataatattt cggtaatata tatatgagag gttatttttt atgaaaaaag caatttctat
     2461 atttatgacg gttatatttg ttcttttatg cttttcctct tgcgcactca ccaagtcgaa
     2521 atggttatta agaattacgg aacggtaaaa atcgagcttg acggcgataa agctcccgtt
     2581 acggtcgaga atttcgtaaa tctttgcaat aacggctttt ataacgggct taccttccat
     2641 cgctatgtta aagattttgt acttcagggc ggcgaccccg agggcactgg tctcggcggc
     2701 tgtgaaagca gcattatcgg agaattttcg gcaaacggac acaaaaacac gatcaaacac
     2761 aagcgcggag taatatcaat ggcgagaaac caatttgatt acaattccgc ttcgtcacag
     2821 ttttttattt gtctcaaaaa ttcgtgctcc gaggatcttg acggccagta tgcggcattc
     2881 ggtcgaatca cggagggtat ggatattatt gataaaatat gtgagaatat ccctgagtcc
     2941 gattgtattc ctgccgatcg gcagcccgtt attgaatcaa tgaaggttat tgactaagag
     3001 gtgtaaaatt tgaacaacaa tcttgattat ctgaacagnn nnnnnnnnct gaacagagtt
     3061 tatcttagaa ttgaccttga taaaatctgc aacaatataa aagaagtaat caaaaaggtc
     3121 ggcgacgata caaaggttat ggcagtcgtt aaagccgacg catacggcca cggtgcaata
     3181 gaggtcagca aggcgctcag cgagataggt acatacggtt gtgccgttgc gactgtcggc
     3241 gaggctcttg cgctcagacg agccggcatt acaaaaccga tactgatcct tgattttgtt
     3301 tttccgaatc agttcgagac gattatcaga aacgacataa tgctcaccat tttccgctac
     3361 gaaattgcca aggagcttaa cgaagcggca gaacaaatgg gcacaacggt gcatattcac
     3421 attaaagttg ataccggaat gggcagaatc ggttttattc ccgatgagga gagtgttgat
     3481 gagatacgca ggatttctca gcttcccaat gttgaaatag acggtatatt tactcatttt
     3541 gcgtgcgccg acgaggctga taaaacatca atgaacaaac aattcgagaa attcaaaagc
     3601 ttcgttaaaa agcttgaaga aatcggtata aatattccga tcaagcatgt ttgcaacagc
     3661 gccgcaatca ttgacagcaa cgataaattt ctgaacatgg tcagaagcgg aatcattacc
     3721 tacggcctct acccttccga gtttgttcac aaggacagac ttcatattga gcctgcaatg
     3781 gagcttcaca gccttgttat aaacgtaaaa acaattcata agggcgatac cgtgagctac
     3841 ggctcaacat atgttgcaaa aaagccgaca gttattgcaa cgattcccgt cggatatgct
     3901 gacggatatc cgagaatgct gtctaacaaa ggaagcgtta ttattcacgg caagag
//
LOCUS       DAWBSW010000183         3955 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3880_length_3955_cov_2.528718, whole genome shotgun sequence.
ACCESSION   DAWBSW010000183 DAWBSW010000000
VERSION     DAWBSW010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3880_length_3955_cov_2.528718"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1139 a          905 c          987 g          924 t
ORIGIN      
        1 gtatgggaat aacctttgac gtcggcagaa cccgttcgtt acatgctatc aaagctgcca
       61 tggcggatga tcagcaaatt ttccttgttg ctcaaaagga cgtcagggat gatgagcccg
      121 actttgataa gctttttaaa ataggtacaa ttgcaaagat cagccatatc ctgcgcctgc
      181 cgaacagcga cacggttcgt gtttctgttg acggaatcac aagagcaact atggttgata
      241 tgcttcagtg cgacccatat ttgattacgg acgttaaaat ccgccgtgaa atcagcgtca
      301 aatcggatga caaggactat gcgactgcac ttgttcgcca aacaaaagac tatttcgagg
      361 actatgccga ggttgttccg cagatgcctg ccgagataat tctcgacgtg ctggaaaaga
      421 acgatccggg cgaactcgcc gactatatcg gttcaaatat catgcttgaa tacaagaaac
      481 gccagcagat tctcaacgaa atcaatccgc tcaagcgtct tgaaaaggtc tgttgtctgc
      541 ttgccaacga gggcgacctg atgaagctcg aaaacgagat aaatcaaaag gttcaggaaa
      601 atatcgacaa gagccagcgt gaatattatc tgcatgagca gatgaaaatc atttccgagg
      661 agctgagcga cggcctttct ccgcaggcgg aatgtgacga attcagagaa aaaatcattg
      721 cactcggtct tgacgaaaaa tccgaaaaga agcttctcaa agagtgtgca aggctcgaaa
      781 aaatgtcctc aacgtcaccc gaggcgacgg ttatcagagt ttacctcgaa acctgtcttg
      841 agcttccgtg gcataaatat tcaacggaca agcttgacct tgctcatgcg agaaaggtgc
      901 tcgaccgcga tcattacggt ctcgacaagg tcaaggaaag aattatcgaa gctcttgccg
      961 tgcgttcgct ttcggacggc tgttacggtc agacgctttg cctcgtaggc cctccgggtg
     1021 tcggtaaaac ctcgattgcc aaaagcattg caacggcgat gggcagacgc tttgcgagaa
     1081 tttcgctcgg cggcacaagc gacgaggctg aaatccgagg ccacagaaaa acctatatcg
     1141 gcgcaatgcc aggcaggatt ataaatgcag ttaagcaggc aggaactcag aatcctatca
     1201 ttttgcttga cgaaatcgac aaactcggct ctgactacaa gggcgaccct tcgtcggcac
     1261 tgcttgaagc tcttgacggc gagcaaaaca gcacgtttgt tgaccgctat atcgagcttg
     1321 aatttgacct gagcaaggtt atgtttatca caacggcgaa cgacgcttcg actatccctg
     1381 ctccactgct tgacagaatg gaaatcattg agctgccggg ttacacgaat gaggaaaagt
     1441 ttaatattgc taagaagcat cttgtaccta agcaggcgaa gctgcacgga ctaaacggca
     1501 gaacgttcaa gataaatgat aaggcacttt atgcgctgat tgacggctat acccgtgaag
     1561 caggcgtaag aaagcttgag cagaaaattg ccgcactttg cagaaaggcg gcggcggaga
     1621 ttgtcggcgg cgaatcaaaa tcgcttaccg tcaaggaagc caaccttgaa aagctgctcg
     1681 gaccgaaaaa atatcttccg ctctcggttg ataaggaagc tgaaatcggc gtcgtaaacg
     1741 gacttgcatg gacgagcgta ggaggcgaaa tgcttcaggt agaggcggca gtctttgacg
     1801 gaacgggcaa ggttgagctt acaggctcgc tcggcgacgt tatgaaggaa tcggcaatgg
     1861 cggcggtcag ctttatccga agcaaggccg acaaatacgg cgttaatcat aatttctata
     1921 aagaaaagga cattcatatt catgttcccg agggtgcggt tccgaaggac ggcccgtcgg
     1981 caggcgttac aatggcaacg gctctgctct cggctctcac gaatacccct gttaatcagt
     2041 ttgtagccat gaccggtgaa atttcactta gaggacgtgt tctcccgatc ggcggcttgc
     2101 gtgaaaaaac aatggcggct tacagaatgg gaatcaaaac ggtcatcatt ccgcagaaga
     2161 acgttcccga cctcagtgaa attgatgaaa aggtcagaac agcattgaaa ttcgttcccg
     2221 tcaccgaatt ggacgaggtc tttgagattg cacttgtgac acccaaggaa gaaaaagaaa
     2281 gaaaaagcat cgccgcagta gcgaagaaag attccggcta cacggcaatg cgaatctgag
     2341 gatagacaaa tgagatttga caaagtaaaa tatgaggctt catacggcac agctcagcag
     2401 ctccctgcga gcgagcatat tgagattgcg tttgcgggac gctcaaatgt cggcaaatca
     2461 tccatgctca ataagattct taacaggaag aatcttgcga gagtcagctc cgttccgggc
     2521 aagacggtca cggtcaactt cttcgactgc gacggaataa agctcgttga cctgcccggc
     2581 tacggctatg caaaagttaa tttcaacgag aaaaagcgtt gggctgacct catggagggc
     2641 tacttcacct ccgacagaaa cattcgcctt gtcgttcagc tgactgatat gcgtcatccg
     2701 gtaacaaatg acgaccttga tatgatgcgt ttcatgcagt cggcaggcta tgatttcatc
     2761 gttgttatga caaagagcga caagctcaac aagaccgagc gtaccaagcg catggaagat
     2821 atccacacgg agcttgcgga gttcggcgat gtgaaaatca ttccgttctc agcctccaac
     2881 ggcgagggcg ctgacgaaat ccgcaaagca atcgaagccg ccgctgaaaa ataaaaaata
     2941 tataactatc tccccgatta catccgtagt cggggagatt tttggtgcaa tttttcggtt
     3001 taaaacgttt gataatatta caattctctg attatgtcta ataagtgctc gccataattc
     3061 ttaatcagac ttttattctt gacttttttt aatgaacatg ataattttta attggtcaat
     3121 aattgatcaa taagattaaa aggtggcgaa aactgtgaaa aacaaatttt tcaaaaagct
     3181 catcagcctt gtgctctgcc tttgtattat ggcattttgc accgttgtcg gctttgcaaa
     3241 aggtgaaaag aaaagcgatt atccgttcat ctatgtacac ggaatgtgcg gctggggcga
     3301 cggtgcaccg caggaagaga acagaccgta ctggggtact cagccggaaa acaatgtcat
     3361 gacttatctg cgtgcacagg gttatacagt atacaatccg acggtcggcg gttacagcag
     3421 cgcatgggac agagcgtgcg agctttatgc acagcttacc ggcactgttg ttgactacgg
     3481 tgcggcgcac tcaaaagaat gcggccatga cagattcggc agagattaca ccggcagggc
     3541 aaccatggga aaagcatggg atcttgagag cccgcttaac tttgtcggtc attccttcgg
     3601 cggcgaaaca atacggcttt tggcttcgct gcttgcttac ggcgatgaaa cggaaatgaa
     3661 agcagccacg gaaaactgct cggagctttt caagggcggt catgaaaagg gcatccgctc
     3721 aattacagcc atctcctccc cgcataacgg ttcgacggct tcaaactatg ttgccgacac
     3781 ctaccttccg gcttttctta tgatatttat gctccattca aaatgtgttt ccggaaaatc
     3841 ggcaaatgac cttatgcttg accaatgggg aatttccaaa gaccctataa gcggagaaaa
     3901 agcaaaatta aatccgtttg cgtgcatgaa aattgccttt tcaaatgatc actgc
//
LOCUS       DAWBSW010000184         3947 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3885_length_3947_cov_3.177801, whole genome shotgun sequence.
ACCESSION   DAWBSW010000184 DAWBSW010000000
VERSION     DAWBSW010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_3947_cov_3.177801"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    291..300
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3535..3544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1216 a          761 c          944 g         1006 t
ORIGIN      
        1 caataattga cgggtgagga aacgcaaatg acagtaatcg ttattcttct tttaataata
       61 attgcaatac tcggctacga tacgtgggag gatttattta catggctcag ccgtataaaa
      121 atcggacagc tccccgagaa tgagtggcga gaaaaaacac agaaaatttt acttaaatgg
      181 attgtgaagg gcgctccgga ggttcgcata aacgataaca gaaaagttaa ttttataaaa
      241 aaaataaacg agtacggtaa aattacctcc gtaacatatt ggcaggatgc nnnnnnnnnn
      301 cccttctgaa agcagccgac ggaataaaaa aagacgtcgg aacagaggta aaaggccttg
      361 cgtcgagata tatttctcaa gatgacggcg agtggaaaac tcttccgaaa agaatggaca
      421 gcgcaatgtt ttgctatgag cttctttcaa gcaagtacat tgacagcgaa acaatcaagc
      481 cggcaatgga aaacacggca tatatgctca agctttccgc agagaaatac ggctcaatac
      541 cgtataatga aaatttcccg aaatacagac ttgttgacac aataggaatg gcgtgcccgt
      601 ttttgattaa atatgccgtt acttacggcg aggacgaata tatttccctc gcaatggatc
      661 agataagaga atacagaaaa aacggcattg aagaaaaaac gaaattaccc ttccacggct
      721 ttggtgaaga ctcacttgaa ccgctcggca tatgcggctg gggcagaggc tgtgcatggt
      781 gggcaatagg actgactgac agcctcagag caatgcttga atcggacggt cacaaccgtg
      841 aaaaggccga gcttttgaag ctttctgtgg aattctttga tgcaatgaaa aaatacattc
      901 acgaggacgg aaccgttgat cgaattgttt taagcttttc gatacaggac agctcggcgt
      961 gtgcgatgct ttcttattgc tatgcatata tggcgaagct tcttaaaaac gaggaatata
     1021 aggctatttc cgagaagatg agagaaaagc tgaaatcggt aacaagacga aacggcacag
     1081 ttgatttttc tcagggagat acccacggaa taggttttta ttcggaaaag ctttgcatag
     1141 tacccgccgc gcagggattt acaatcgctg ccgacaatct tttaaatgaa ttgaactaaa
     1201 acaaaaagga ggagcaggta tgattgatct tcactgtcac attcttcccg gaatggatga
     1261 cggatcgaaa aatatggatg tgtctgtgca gatgtgccgt attgccagac agaacggaat
     1321 taaacatatc gtttgcactc cgcatataac aacagtgggg gatatagaca gttttgtaaa
     1381 gataaggaac gaaaagctcc gcgatctgca gggaataata aacaatctcg gtctcggaat
     1441 aacactctat cccggggcag aggtctatgt cgatgacgat atttttttct cgaccgatct
     1501 caggcgcctt gcgataaacg gcagcagata tattcttgcg gaatttgcat tcagagatgt
     1561 atcaatgaaa agaattgtca actatcttaa tgcgctgatt gatatgggac ttattcccat
     1621 tgttgcgcac ccggaaagat atgagtattt ccagtttgac tatgacgagg tcaacacgct
     1681 gataagaaac ggcgtgctgt ttcagataaa tgcctcaagt cttgcgagtc ttgacggccc
     1741 gcaggagttt gagcttgcct acgcaatggc atatacggga gcggcgtcat tcattgcgac
     1801 cgacgcacat tgatcccatt acagaatgaa tgatctgatg aatatgataa atttcttccc
     1861 gcccgatatt tctcagttta atatgcagat gatggttcac gatgcggcaa aatgtgtgct
     1921 cacggacaag gaagtgccgc ttattaacag aggagaaata tataaaagag gtttttgagg
     1981 ttgactatta actaaatata aactataata aaaaatggag atgatttgaa aatgcaggaa
     2041 ctggatgtcg gcaagaatat atattataat gaatcaggtc cctcgcttga tatgggtcga
     2101 ttagcaaaaa atctcatatc aaagctttgg attatattgc ttgtcggcgc tattttctcc
     2161 ggtgccgcat ttgcaattgc caaggctact tatgttgaat cctacagatc ttccatgaca
     2221 ttgggcttta tgacgactca gtacgttatt gttaaggatt attctaacag caaggattcg
     2281 acgacgcagt acaagcagga gacaaagttt tcctcgggca atcaaatttc atattatcag
     2341 tatcttatca aaggcgacga aatgctttat aagattcaaa aaactcttgc agaggacgag
     2401 gtcggaaagg aatactccac ctcatttatc gaggggagct tatcaattga ggaaacagga
     2461 atagacggat ttttccaggt taacgttaca agtacggatc agggctattg cgaaagagcc
     2521 ttgaaggtcg ttcttaaaga gttccccgag tatgttcaga gctatgacaa cacaatttcg
     2581 ataaaggtcg tcaaaaatcc gacggcaccg acaattgcaa actctgacga tgcaacgaca
     2641 aaggcaattt acggctttgc tatcggcgca gcgatagttg catttattat tttcattata
     2701 acaatcacaa caaatacggt tcttacgctt gacaatttaa gacgcgacgt caatgttacc
     2761 gttctcggtt ctgtgccgtt gctggagaaa aaatcgggat tgtttaatca gaagaaaaaa
     2821 tcgccgcagg gctctctgct gattacggat acgtcaaagg tgagctttgc ctttgtggaa
     2881 agctttaagg cgataagaac aaaaattgaa aacgtcaaag ccgaaaaagg ctacaaggca
     2941 tttgttgtta cgagtaccta tgaggatgag ggtaaaacga ccgttgcagt aaatattgcg
     3001 tgctcactcg cccagaaggg aaagtcggtc cttcttatcg acagtgacct cagaaagccc
     3061 gcggttcttc atgccgtcgg cgttaagagc gacacacgtt acggtcttat cccgataatc
     3121 aagggaacgt caacctatgt tgattcaatt aaatttatca aatctctcgg tattttcgta
     3181 ttgccgacgg gaggtatctc gctgaaatcc accgaggttc ttgatacgga taaggttaag
     3241 gcggttattg agcaggctcg caaggagttt gactatatta tcattgattc gcctccggct
     3301 catgtcgtgt cggatagcct tgttattgct ccgcttgcag acggaataat ttataatatt
     3361 cgccgcgact atgcaaaggt ttcggaaatc aaccgtactc ttgatgaaat aaggggcgcg
     3421 gatattgata tcatcggagc cgtatttaca atgagtgccg gcgaagaaaa cagcagatat
     3481 tatcgtcgta agggttatct gcgttacggc ggatactatg gcagatatgg caggnnnnnn
     3541 nnnnacggct atggaagcgg ctacggctat ggcggctacg gctacggcgg ctatggttac
     3601 ggaaacggct acggttatgg ctacggctac ggaagcggtt acggctatgg cggaggttac
     3661 ggctatggaa ataccccgca gaacaaagaa ccggaaaagg gaaaaaagat agataacgaa
     3721 taaatttccg aaaagaagtg tgtcagatga acaaaagaat ttatctttcc ccaccgtgca
     3781 tgaacggaac ggagattgaa tttatcaacg aggcctttca gacaaactgg atttccccgt
     3841 tcggacctaa tttgacggga tttgaaaaag aaatgtgtga atatgtcgga gcaaagtaca
     3901 gcgttgcgct tgcttcgggt accggcgcaa tacacatggg cttgaag
//
LOCUS       DAWBSW010000185         3907 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3920_length_3907_cov_1.618899, whole genome shotgun sequence.
ACCESSION   DAWBSW010000185 DAWBSW010000000
VERSION     DAWBSW010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_3907_cov_1.618899"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    310..319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1224..1233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2454..2493
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3094..3103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1081 a          824 c          786 g         1146 t
ORIGIN      
        1 atcatatgat agttgaagtt gaatcagact tcgccgatat agaaggaaac actccgggca
       61 ggtactttat tgagatacaa cttaggacca ttgcaatgga ttcctgggca agtcttgaac
      121 acaagcttaa atacaagaaa gatatttcca atcaggagct tatagtcgcc gaactgaaac
      181 gctgtgccga tgaaatggct tcatgcgatg tttcaatgca gactatacgc aacatgattc
      241 acgataagca attctgagaa gatcggagga attaatatga ggattctcct tgcggaagat
      301 gaaaaggagn nnnnnnnnnc tttcacgcgc tctgacagcc attcttgagc attcaaaata
      361 tgcggtagac gctgtatacg atgggcttgc cgccgttgaa agtgcggaaa ataacgctta
      421 tgacgctatg atatttgata taatgatgcc aaaacttgac ggcattgagg ctttgaagca
      481 aatacgctct cacggaaact ttacacccgt actgctgtta acggcaaaag ctgaaatttc
      541 ggacaggata gtggggcttg acgcaggtgc ggacgattcc cttaccaaac cttttgctat
      601 gggtgagctt cttgcaagga ttcgctcaat gacgcgccgc gccggagact atactccaaa
      661 aaagctcaga gtcggttcgg ttacgcttga tatggagcag caggagcttt ttcatgaaaa
      721 cagcatacgt cttgcagcga aagagaccaa gcttatggag tatcttatgc ttaatgaagg
      781 caaaaagctt tctacttccg aaatacttac ccatgtctgg aacggcgagt ccaccgatga
      841 aaaaattgtc tggatgtatg tgaattttct gcgcagcaag cttgattcga ttaatgccga
      901 catcacaata gacggtcagc agggcggaag ctacgttctg tcaacgcttg caggaggaaa
      961 tattgcatga taagaaggct gaggcgtaac ttcatactca tagcatcttc ggcaatactg
     1021 ctgatccttt cggtgattct gcttgttgtg aatactatca gctacatatc aaactactat
     1081 gaatcaatgt caattctcga tattatttcc gataactgcg gccgtattcc cagcgatgag
     1141 caatttgacg gattcagctt tcaggtaagt cgggagcttc cctatgaaac aagatatttc
     1201 acacttgttt acaacagtga tgannnnnnn nnncgtatac atcttctttc acttatggca
     1261 ggaatagggt tctatctggt gtttgtgctg ctggtttcgg ctctttcaaa tcgtgctata
     1321 cagcctttta ttgaaaccta tgaaaagcag cgtcagttca taacgaatgc agggcacgag
     1381 ctgaaaactc cgctcgctat tatctcagct aatactgagg tcatggaggc tatgaacggt
     1441 aagtcagagt ggactcagag caatatgaat caggtaaaac gattgtcagg gcttataagc
     1501 gacctcataa ctcttgcgag aatggaggaa gcttcggaga tgaatgaaag cgtcaatgag
     1561 gagatcgacc tttcaacgca gctcggagag attgcatcct cgttccgtcc cgttattgag
     1621 cagcagggaa aaaagcttga aatagacatt gatgacggca taagaataaa gggcagtcct
     1681 aaatctatgc atgaacttat ttcaattttg cttgacaatg ccgcaaaata ctgtgacgac
     1741 ggcggaattg tccgcgtaga gcttcgtcaa cagcgtaaac aggcaagtat tgtaatatca
     1801 aacagctaca aggagggtga caacgtcgat cacagccgct tctttgagcg cttttatcgc
     1861 gaggatagtt ctcataacag cgaaaaatca ggctacggaa taggactttc catggcagag
     1921 agcatagtaa atctccataa aggaaaaatc acagccgacc acagcaacgg aataacatat
     1981 ttccgcgtat taatataaac gtaaaaagca gcagtttaaa actgctgctt agtctatata
     2041 aattttctat ttaactaata atgggattct aaatggttta taacccttta gctaattcca
     2101 aaggcagagc ctttggtggg gtgtggggca gaattgttac aacagttttt ccctctccga
     2161 gcttttccgc aagctttaga gcggcggcaa catttgtacc gcttgaaatt ccgcacatta
     2221 tgccctcttt tactgcaaga tccttggctg tctgtaaagc ttcctcatcg gtaacaatgt
     2281 aaatatcatc gtatatctca cgattgagaa tatccggtat aataccgtca cctatgccca
     2341 tttgaaggtg tgtcccgata gttcctcctg cgagaattgc cgcgtgttcg ggttcgactg
     2401 cccatatttc aatgtcagga tattgagact ttaatgtctc acctatgcct gttnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgcctg taccgatacc ggagcaaaat
     2521 ccgtcaattt taccggcagt ttgctcaagt atctcaagag cggtatgata cttgtgagca
     2581 tttgtattat tttcatttgc aaactgctga ggaacaaaaa cattctcatc ttcttcagcc
     2641 attcttagag ctgtttgcag acactcatct atgcactttc cgatatctcc tgcatcatgg
     2701 atgagtatta cctctgctac gtaatgcttg acgagttttc tgcgttcttc acttactgaa
     2761 tcaggcatta taataatagt tttatatcct cttaccgctc cgacaagcgc aagacctatg
     2821 ccctgatttc cgcttgttgg ttctacgata atggtatttt cgttgatttt tccctctttt
     2881 tcagcctgtt ttatcatatt gtaggcagtt cttgttttta tcgagcctcc aacattgagt
     2941 ccttcaaatt ttacaagtat ctcagcattg cccggcttgt tcattctgtt tagtcttatc
     3001 atcggtgtat gaccgatcgc atcaaggata ttgttgtaga tcatagctat ctcccaccta
     3061 aataaattgc cgaaatatag tatatcacaa aagnnnnnnn nnnagtcaaa taccagaagc
     3121 agagccattt tgaatctgct ttctgccttt acagaatgct ttccgagttt gtcaaacttg
     3181 aaagtctcgc ctgctttcag ggggtaatct ttgccctcat agcctatgac cgcctcgccg
     3241 tcaagtgcaa aaacgagtgc ttctccgggt gcagcgtgtt ccgataagcc cgttccggca
     3301 tcaaagctca taataatgaa cttcatttta tcattgttta ccaagtccat gtttacgatt
     3361 tttccttctt ggtacggaag aagctctcct aatctgaaaa cttctcctga tttgatgatc
     3421 tcattcataa tatcgtcctt tctaaggctg atttccgtga aaacacagct atttttactc
     3481 tttattccaa cagatatgtc aatatctgtc acaatactgt tattatcttg tgatcctcat
     3541 agttttcagc acttatgctt gttccctctg ccattgaaaa aacaaaaacc gacattccgt
     3601 tttcgttaca gagctgttcc gaaatagtgc agccttttac aggttcattt tctttcggaa
     3661 aatttataat acttcccgat gattttctga tgcccattat caaaaagcgc tcctttcctg
     3721 taattattat gaacaatatc aggaaaaggt ctgtgatttt cttagtatag aatattttac
     3781 cacaaaaacg cctgatcgta tgttgtaaaa acaacgtttt ttagattttg ccatgcctgc
     3841 aataattaat tcaggtaagt aacataatcg gagttataat attcccaact gtctgccgca
     3901 gtaagtc
//
LOCUS       DAWBSW010000186         3899 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3926_length_3899_cov_2.353278, whole genome shotgun sequence.
ACCESSION   DAWBSW010000186 DAWBSW010000000
VERSION     DAWBSW010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3926_length_3899_cov_2.353278"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          977 a          871 c          857 g         1194 t
ORIGIN      
        1 cgggaatgat tatttgataa gaatatcccg tcatcacgaa caaccgatgc ttgcaacgta
       61 gcttccgccg tacatgttaa ggtagggaac aatctttgct tcaacctcgt catggtttga
      121 accgtaaaca ttcatggttt taaccgtacc gcttgcaaaa tcgtagtaca gaagatccca
      181 gcctttaccg gtaacacctg tgtcaaggtt ccagtgagct gttgcgcttc cggttgcgtc
      241 ggaagcacgg ttcgggtcgc cgcttccggt tgtcgaaaat ccgtccgagc cgagcattac
      301 gtcatatgct tctacgggta catactcatc catgttttat tcctccaatc gtattattaa
      361 agattttgtc gatatatcgg caataatctt tgttaacttt gttaaccgtg ccgctctctg
      421 ttgcaagcgt tgcggcacat ctgggacaag cctttgtcca tttgcacttt tgacattcct
      481 ccggccattg aagcttttct tcaaagtcca caaggcgctt ccacgcttcg gcaattccga
      541 gcgaggcggc gtttatgtca ggttcatcaa gaaaggagca aaaggtcatt tttccgtccc
      601 attttatcca aaagcccgta cggtactcct tgcatttctc gcagggcttg gctgcgcttt
      661 ttgaactttt ttctttcagc aaatactctg catcctcaca aagccgaggg tatgcgctat
      721 cgggcagacg aagcgattca acctcggaat cggcgcctct gacggattta cgcaaacctg
      781 cggaaaaagc ccatggaatg tcgagatttt tcataagctc ttgaatttcg ggcaagtcgt
      841 caacattatc tcttgtgacg gtcgtaacgg cctcaagcga aattccgaga gactttataa
      901 gcttaatgtt ttttgcaacc tcggtaaacc cgttgtctgt tccgcataac ctttggtatg
      961 tggcgtcgct cgtaccgtag attgtaaact ttatgcactt cggcggcgat ttcttgaaaa
     1021 gctcaataca ttcatcgctg aggtggtata gattgctttg cagggttatt atgaagccca
     1081 tacgcgaaag ctcgtaatat attttgcaaa aatcgggatg tctcgtcggt tcaccgcccg
     1141 ttatgcaaag gaacagtgtt cccgattgca ttgcttgacg tccgagttct atccattgct
     1201 ccgcgccgag ctcttttccg tgagattcga tttcgctgtc cttgatatgc acgtagcaca
     1261 ttttgcaatt aaagttgcat ctcggcgtta actcaaagga agcaaacaac ggaacagcat
     1321 tctttcttgc gtcaatgagc atttcaaacg gagctgccgc aatcggaagg cttgtgcttt
     1381 cattcatgcg tcatcactca tatccaatat attatttctt gcgagaattt gcaggaaagt
     1441 gattatatcc tcctgcgcct tttctcgggt gacgtttttg tattttgaaa tgatgaggtc
     1501 aacaagctcg tcaagagtga attccctgcc ttccatatgc tgccagagat atgcggcgga
     1561 ggaggaaata taaaccatag acggcacttc cttaacacgt tccattatgg gaacaatgat
     1621 atactgtccc gctatttcgc gcagaacaag ctgatccttt actctcataa tcatgctcct
     1681 tgacttaatg cttgataatt attttttttc ggttgcttca tcgtaatcgt atttgccgta
     1741 accgtatccg ccgtagctgc gataaccgta tttgccgtac ttgccgtagc gtccgtagcc
     1801 gtaatagccg tagctgtagc cggggtcaag tatgctttcc ttggcggcat tgagaactcc
     1861 gccgataata ttgcagcctc gggttgaaat attgtttatg caatcaagaa tcgtccatct
     1921 gtgggtcgaa tcctgacgga taacgaagag gatgccgtca ctgtattcgg aaatcaaaag
     1981 gggatccgtt gttattgcgc aaggcggcga atcaatgatt acataatcgg caattgctct
     2041 gagctccttc atgaaatcat acattttttc gtcattgagc tttttctccg gacttttgct
     2101 tggagcgtcg ctgccgaaaa caacgaagtc ttttggattt tcattaaata gtcttttgat
     2161 ttttttatca cagctctcgg actgaacacg aagaacctta ttgtcgatga agcgtttttt
     2221 aacagttctc gtctcaccga ggagcttgct gagcgaacaa tagtttttct tattctcaag
     2281 ttctctgctg actgaaagct ttctgagatc gaggtcaata agagcaacct ttttgtttgg
     2341 gttacccgcc ttgatatagc gagccaaatt gatggcaact gagctttttc cttcgtttgc
     2401 aagtgcggca gtgaccataa gcaccttggt gtccgtcttt tgcattgagc gaactacctt
     2461 gagcgaaaga gccttcatgc tttcggaata tctcttgccg ttttctgtgc tctcatcggc
     2521 atagataggg tgtgaacctc tttttttgcc gacctttatg taaggaataa tgcccatgca
     2581 gttcatattt aacttatcgt tgaaatcctg ttctgtctga attgtttttc tgaacagagc
     2641 aaaaattatt ataatcaaca agcttgcgac aaaggttatt gcaaagcaga taataacgga
     2701 cttcaaatag ctgattctgt tgttcggctg cgtcggaacg gagctgggtt gaacgatatt
     2761 cagctttgtt tcgccgataa cgtatctcga aacgtcggga agacgtttga cagcggcatc
     2821 gagaattgcc ttggcgtcat cgggattttg actgacaaca gaaattgaga aaagatttga
     2881 atttttgatt gcattaagcg aaatcgttcc gtttattctt tcttcaccga gctcttcctt
     2941 cagaatctca ttgaaaacgg agcttttgag aatataggga agaaccttag ccatctgctc
     3001 ggcgttggcg gaatcatagt agaagccgta tgaagcggct atttcggcat atgaggtcgt
     3061 cgtattgacc gtgaaagatg ccttggattc ataggtggga acatatgtat atttctgata
     3121 ggcgcagaag attacggaaa cggcaacgaa tgccgaaacg cagatccacc acgctctttt
     3181 gaatattttc agaacatccc tcagcagaac atgaaggtca aggacatcat cattatatct
     3241 gtttgaattc atattactca ttttcgctcc tcaatgtagt tgttttcggc tgttttgccg
     3301 tattcgtagt tttcgtaatg gctgtagccg taaatgctgt cgccggctac atgcatatta
     3361 aattcacgat aatcattgag aacataacct aaatattttg cgttgccttc cttaatatct
     3421 tcaatggcgg tattgatgtc ggaaaggcgt acataatcgt gacggacaac tatcagcgag
     3481 cagtcaacga tatccgaaac aagctgaacg tcggttccta cgattattgg ggatgagtca
     3541 agaacaatat agtcatattt cttatcggcg taatcaagaa gctcctgaag ccttactgaa
     3601 tgaatatata cggttgactc tttctttgaa ccgaagttca ttatcatatc aacgcccgtt
     3661 cttgaaaact tgcgaatgat atcatcaaaa tccgactgtc cgtcgatgta cgaaataagg
     3721 tcgggaattg acttcttttc cctctcggtg attttataaa gcgcgggttt cctgaaatcg
     3781 acatcgacaa gcagaaccct tttgcccata ctgctcaggg caatggcgag gttggtggaa
     3841 actgttgatt tgccctcgtt ttctgcaacg cttgttacaa gcacacgctt atatccgtt
//
LOCUS       DAWBSW010000187         3857 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3977_length_3857_cov_2.319832, whole genome shotgun sequence.
ACCESSION   DAWBSW010000187 DAWBSW010000000
VERSION     DAWBSW010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3977_length_3857_cov_2.319832"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2847..2856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1025 a          863 c          880 g         1079 t
ORIGIN      
        1 atatatgata aacgaatttg tcgttaggtt tattccgatg cttttcggat ttaaaccgat
       61 acatatgaaa atcgagtatg atgaaggagt tgtagaggac gatttttatt tcggctcaat
      121 ttccaattca acttctgtcg caggactttt tcagtttgac catcaggacg ccaagctcaa
      181 cgacggatat ctggagctgt ttatggttaa gggcttgaag cgaaacatag acgccttgaa
      241 aatgctgaaa aaagcaatag ctcaggacta tgacggagtt cagatgatga tcgttaaaac
      301 caaaaaggtt aagatcacct gtaatcagga tgttccctgg acactggacg gcgagttcgg
      361 cggacttcac cgtgactgtg agcttgaggt tgtccacgac gcatttgaaa ttttctccga
      421 gaataataaa cttttttgtg aaaaagattg ataaataata taatccccga aagtgttgca
      481 atacgctttc gggggttata attctgagca attacttaac aataaactct ccgctcctaa
      541 tagcaatttc attttcaccg cctgcgaatt tcgcaccgtg gcggagcttt atttttcctg
      601 ttatgccgtc gggaacggtg acattcagct tgatttcatc gcctgtgcgc tcccagcgga
      661 cggaaatttt tcctgccggt gcggtgtacc aaccctcggc atagctcagc gagccgacga
      721 aggcaggaga tatgacaata taattcgggt cgcatacgtc ggcgttcgga atgatacctg
      781 caaggttctt gatgaaccac gccgaaatat caccgaagaa gtggtgattg tgtgaaaaac
      841 cgttcggcgg atcatgtgtg atacattcaa agagagtgtt tgcgttgtat tccgttatcc
      901 agcttgcaaa tgacgggtaa tcgggcttcg ctatcatgtg aagcgccaga tcctcgtagc
      961 cgaactcggc gagaacatgg aacatcgttc ttgcgccgat aattccgaca gtcatgttgt
     1021 cgtttgcatt atgaataagc tcgagcaggt gagcgaatgc gttttctttt tcactttctt
     1081 caaatgcgcc gtagtaaatt gccgccgcct gggttgtctg atggttgccg taaacaacaa
     1141 gattatcgtc aatgagatgc tttcggaagc ttgcacgaag ctcgtcatga agcttctgag
     1201 caaattcggc acgaagcggc atatcgcata ctctgaaaat ttccgtcgct ttttcacata
     1261 tgcccattga aacaagtgta tcggtaacct caagagggca gggcttaacg ttgccgacag
     1321 gcagccagtc gccgagaccg atatgaacaa ggcctttttc gtcaagcctt gtcgataggt
     1381 acgaaacata gcggaaaata gctgtggcgt tttcctcaag aaccctcttg tcgcctctgt
     1441 actgccatat tctgtacggc agctcggtaa gaacagcatc ccacgcagga ccgttgcccc
     1501 attcaaagcc ccagccgtcg gtcggtacga ttccggggag tgcgcctctc tcgtcctgcg
     1561 attttctgac gctgtgaagc cattctcgaa gcgtattttc cgtcccgaga ttcatcatta
     1621 actgctcggc ggagaggtga gcgtcgcccg tccagccgtt tttctcacgg tggggacagt
     1681 cggtcgggaa ataaaacata tttgcaaggt cgctcctgag cgttgcgtcc tgaagcctgt
     1741 tgacaagctc atcggagcag tcgaagccgc cgatctgcgg aacgtcggcg tgcattacaa
     1801 cgtaagtgag cagatcttct gttgcctgct tgtcgtcaat acctgtaaca aagcagtatt
     1861 ggaagccgtg gtagcagaac atcggctccc agatttcctc gccctcgcct ttgcagatgt
     1921 aaacctgaac ctgcgtcatg ccgattggct gaaattcgca gatattgtgc atatcaagtc
     1981 cgccgtcgaa gagactttcg ccgcaggaca tgacgatttt ctgacccttt ctgcctctga
     2041 ttttcagtcg cacaataccc gtaatatttt cgccgaaatc atatatgtag ccgtcgttgc
     2101 agtattcctc ctcgggaaga atgtgctcgg ggagattctc tctcgggtga gcgattgctg
     2161 aaatatgtcc cttatagaat ttaacgggct tgatctcacg gatcttctta attagcgcat
     2221 gaccgccctc aagacattcg ccggacggag cctcaacgga tatagcattg ttccactcac
     2281 tgtcgtcaaa atcgggaaga ttccagccgt cgatctcatt tcttgcatcg tagcgttcac
     2341 cgcagcgcat atcgtcaaaa taaatcggcg aagcgtgaac cttaaagctc tcgtcggcct
     2401 caaagcatat tttcttgccc tctccggaac actcaaagca taaggcaagc ttcggcgcac
     2461 ttctccagcg ggcaagctga aaatcccaaa cctcgccgcc gatcggattc tgcaagccgt
     2521 tgccgagagc aaaagcaatg acgtttttgc cttttttgag cttgtctgtt aggtcatagc
     2581 ggtcataata aatcaggtcg ttcgggttgg agatgtaggg tgcgaggcaa ccccttgtaa
     2641 gatgttcgcc gttgagccag aactcataga agccgagacc ggttacaagc atttctgcct
     2701 tttcgggaat aaaatcgagt tcaaatgttt ttctgagata cggtgcagga acaaaatgca
     2761 gaaattcgca gtattccttt gcagcggata taaattcggt gctgtatttc attatgaata
     2821 aacctccaaa aagaaaaagg cagaatnnnn nnnnnnatct aaatttaact tagattacat
     2881 gcctttcttt gattaagtta attttttatt tcttcggttt caatacattc tacgtgttcc
     2941 tcgggagttt ccatgatatt cgaatgattg acaataagca taggaatcat ggtcagtgtc
     3001 aaaacaacta tttcggctgt tccgccgagc cttaccttga gataaattgc ggcaaagcat
     3061 atccaaagga aaaacacgat tgtgaggttg aagaattttt tgtttgttct tgccttgtaa
     3121 acaagtaagt agattaccgc cgttgcgata catataccga acagcacagc gaaagcggaa
     3181 tgtaagctct ttttggataa gaagaaattt atcagattcg tctgcgtgtc cagagtttga
     3241 tccgcataat cgcttgtgta cgacggggcg gtatatgttt cgtaattaag tataagcgta
     3301 aatcttttca gcttctcgtt tttgcacatt accacactga acattgcaaa aagagcaata
     3361 atcgaaagag ctttcggaaa cttgcgctca acattgaatt tttcgagcga atagtttagg
     3421 tttaatacga tagaggctgc gatgagcatt ccccaaaata taaaagcgat ccagtggtct
     3481 ctgccgacgt tgcttgccgt tgaagcacgg tttccgtaaa gagggttagg ttcaaggagc
     3541 cggtcataca gccacatata aacaatcgta actatgccga aaatcaaaga agcatatttt
     3601 aaagctttgt tttctttttt taccattgct acacctcgtt tgaattaaat ttcaagttaa
     3661 ttctatcatt ttaaaaaaga tttttcaagg aaaaaacaaa actgtgataa attttttata
     3721 tttttgtcaa gaaatgtcta aatttcagaa cgctttccgc cctgaacatg gattttcttt
     3781 cttgtcgcaa gctccagaat cggcccgtcg gaaacgagat tatccgtata aacgtcacgg
     3841 aattcgtaat cggcagc
//
LOCUS       DAWBSW010000188         3850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_3988_length_3850_cov_2.734124, whole genome shotgun sequence.
ACCESSION   DAWBSW010000188 DAWBSW010000000
VERSION     DAWBSW010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_3850_cov_2.734124"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1062 a          884 c          942 g          962 t
ORIGIN      
        1 ataccccgtg atcgtccgtc ctgcattcac actcggcgga acaggcggtg gaattgctca
       61 gaacgaggag gaactccgcg atattgcaac aaacggactc cgcctttcgc ctatcacaca
      121 ggttcttatc gaaaagtgca taagcggctg gaaggagatc gagtttgagg ttatgcgtga
      181 cagcgttggc aacgttatcg ctgtctgctc aatggaaaac tttgacccgg tcggcgttca
      241 tacgggcgac agtatagtta tcgcgcctgc cgtaacactt tcggacaagg aatatcagat
      301 gctgcgttcc gcctcgctcg atattatcac cgagcttggc atcgagggcg gctgtaactg
      361 tcagtttgca ctcaatcccg agagctttga atattccgtt atcgaggtta acccgagagt
      421 ttcacgttca tcggctcttg cctcaaaggc gacaggttat cctatagcaa aggttacaac
      481 caaaattgcg ctcggatata cacttgatga aatcagaaac gacatcacgg gcaaaacctg
      541 tgcctgcttt gaacctacgc ttgactatgt tgttgtcaag ttcccgaagt ggccgtttga
      601 taaattcgtc ggtgcaagcc gtaagctcgg cactcaaatg aaggcgaccg gcgaggtcat
      661 gtcgatcgct ccgagctttg aaatggcggt tatgaaggct gtcagaggcg ccgaaatttc
      721 acttgaaaca ctcaattata ataaattccc cgatgacatg gacgttgtcg aacgcttgca
      781 tgaacaggac gatttgcgaa ttttcaccgt gttcaaggct cttaaagagg gtataagcat
      841 tgagaagatc cacgaaatca cgatgattga cgagtggttc ctcggaaagc tcaagaatct
      901 tgtcgactat gaaaaaatgc tcgaaagcgg caagcttgac aaagattact acgaaaaggg
      961 caagaaatac ggctacaagg attcgactat tcttaaaatg gcaggcaagc ttccggaaaa
     1021 gacgcttgac gccgtttaca aaatggttga cacctgcggt gccgagttca aggcagaaac
     1081 gccatatttc tactcgacat atgacagtga ctgcgagtca agagagatcg aaagaggaga
     1141 gaagaaacga atcatcgttc tcggctccgg ccccatccgt atcggtcagg gtatcgaatt
     1201 tgactattcc tccgttcatt gcgtatggac cctcaaggag cttgggtatg aggtcatcat
     1261 cgtaaataat aaccccgaga cggtttcaac cgacttcgac acggcggaca ggctttattt
     1321 cgaaccgctc aacgacgagg acgtaatgaa catcatcaag gttgaaaaac ctgtaggagt
     1381 tgttgttgcg tttggcggtc agacggctat caagcttacg aagttccttg atgaaaataa
     1441 tattccgatt ctcggcactt cggccgaggg tattgatatc gccgaggaca gagagcgctt
     1501 tgacgaattg cttgaaaaat tcggcatcag cagacccaag ggtcagacga ttatggatac
     1561 cgaggacgct atccgagcgg caaacgagct tgaatatccc gttcttctcc gtccgtccta
     1621 cgttatcggc ggtcagaaca tgactatcgc atataacgac accgatgtta agcgttacat
     1681 ggatatcatt ctttctcaga agattgaaaa tcccgttctt gttgataagt acatgatggg
     1741 tacggagctt gaggttgacg ttatctccga cggcaaggat gttcttattc ccggcattat
     1801 ggagcatatc gagcgtgcag gcgtccactc gggcgactcg atcgcagttt atccgccata
     1861 taatatactc gacagcatga ttgacaagat tgttgatatt tcaacaaagc ttgcgctttc
     1921 gctcggcaca aagggccttg tcaacattca atatcttatc tatcgcaacg agctttacgt
     1981 tatcgaggtc aatccccgtg cgtcaagaac gattccttac atcagcaagg ttacaggcgt
     2041 cccgatggtt gagcttgcaa caaaggtcat gataggcaca aagctcattg agcttggcta
     2101 cggcacggga ctttacaaga tcccgccgta tgttgcgatc aaggttcccg tattctcatt
     2161 cgagaagctc aacgatgtca attccgagct tggtccggag atgaagtcga cgggtgaggt
     2221 tctcggaatc ggcagaaccc tcaatgaggc actgttcaaa ggacttattt ccgcaggctt
     2281 caaggttggc catgagagca catatcacga caataaatac aacggcggag tgcttatcag
     2341 cgttcacgat aaggacaagc cggaagttat atcgcttgca aagaagttcg ctgacgtcgg
     2401 cttgaagatt tattcaacgc cgaacacggg caagcttatt cagtcggcag gtatcaatgc
     2461 cgagattatc gaggaattca gcgacggcaa gcacgttatc gaaatgctcg aaacaggcaa
     2521 aatagattat attgtttaca ccggatcaac ggcaaaatcg acgattgaaa acttcatcaa
     2581 gattcaccgc cgtgcagttc agctctcaat ctgctgcctt acatctctcg atacggcaaa
     2641 tgcgctctgc gatattcttt caagccgctt cactcagcgc aacacggagc ttgttgatat
     2701 caatcatatg cgctcgaagc gtatgcgcct gaattttaca aagatgcagg cgacgggcaa
     2761 cgacgatatc tatttcaaca acatgaataa gaatattccg tcacccgagt cgctgactat
     2821 caacttcacc gaccgtcatt acggaatcgg cggcgacggc gttgttctcg ttgaaaaatc
     2881 ggatattgcc gatgcaaaaa tggttgttta taacgacgac ggaactcaga cgcttatggc
     2941 tgcgaacagt attcgctgtg ctgcaaagtt cctctatgat aacggaattg tgccgaaaac
     3001 gacaatgaaa attgaaacca actgcggcgt aaagaacgtt cgtctgtatt cgttcaacgg
     3061 cgaggtaaga tcggctcagg tcgatatcgg caagcccgat tttgatcctg caagcattcc
     3121 gatgaagacc gacacataca tgataatcga ccagccggtt aatataggcg gcgaatctat
     3181 caatatcagt tgtgttacta tcggtaatcc gcattgcatc gtgtttatgg ataatgttga
     3241 cagtgttgat cttaattctc tcggcgacaa gatccgcaat tcgggcattc ttcccgagta
     3301 tatcaatacc ggcatcgctc gaatgattga cagaaacaca atcaagctcc gctgctatga
     3361 gagagcggtc aacggcgaga gcctcggttg cggaacatcg gccgccgctg ctgttgttgt
     3421 tgcaacggag atgggtcttt gcaagaaggg cgaggatgta acggttaaga tgcccggcgg
     3481 cgacgtcgtt ataacctata ccgatgaaac catttttgtc aacggtgaca caagaaaggt
     3541 atatgagggt gtagttgaat attaaaatga tccgtaggtt ttaatagttc ggcattgctt
     3601 taaattaaaa aaaacagcgt ctcctcttcc aaaaggggag acgttaagtt ttaggttctt
     3661 tatcaatagt tggggtagaa aaaaacaaaa ccgccggttc aaacggcggc tttaatttat
     3721 ccttcaaaat cctgatcgaa tatattgaac tcctcgggct cgtttgcttg agcttcaacc
     3781 tcaagcggcg gagtaatctc aattgaatca agctgtttcg cgatttccga aagctcgtca
     3841 ttgaggttgc
//
LOCUS       DAWBSW010000189         3827 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4016_length_3827_cov_2.833775, whole genome shotgun sequence.
ACCESSION   DAWBSW010000189 DAWBSW010000000
VERSION     DAWBSW010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_3827_cov_2.833775"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2752..2761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1028 a          859 c         1025 g          905 t
ORIGIN      
        1 tgtatctgca agatcaacgg cgagtatcag gatctcagaa ccccgatcac gggcgactgc
       61 gagcttgagt ttctcacctt tgaggacgat gaggaggcaa agagaacctt ccgccataca
      121 gcttcgcata tccttgctca ggcagttaaa agactttatc ccgaagcaaa gcttgctatc
      181 ggtccgtcaa tcgtaaacgg cttctactat gactttgacg ttgaaaagcc gtttcttccc
      241 gaggatcttg aaaagattga agccgagatg aagaaaatcg tcaaggagaa gcttccgctt
      301 gaaagatttg aaatgactcc ggacgaggct atcaaatatt atgaggataa gggcgaaatc
      361 tacaaggttg agcttgttaa ggagcacgcc gacaagggcg agaacatcag cttctataag
      421 cagggagagt ttacggagct ttgcgcaggt ccgcatattc ccgatacggg cagaatcaaa
      481 gcgttcaagc tgacttcggc aacgggtgct tactggcgcg gaagcgagaa gaacaagatg
      541 ctccagcgta tctatgcaac cgctttcaca aagcaggctg accttgacgc ttaccttgag
      601 gctattgaag aagcaaagaa gcgtgaccac agaaagctcg gcagagagct tggacttttc
      661 gctctcagag acgaagctcc gggaatgtcg ttcttcctcc cgaagggcat ggttcttcgc
      721 aacactctta tcaattactg gcgtgacgtt cataagaagt ggggctacta tgagatctca
      781 actccgcaga tcatgcgccg ttcgctttgg gagacctcgg gtcactggga tcactaccat
      841 gacgatatgt acacaacggt tattgacggc gaggatttcg caatcaagcc gatgaactgc
      901 cccggcagta tccttgttta cgagcttgag cctcattcat ataaggaaat gccgctccgc
      961 tacggcgagc ttggcaaggt tcacagaaac gagctttcgg gcgcacttca cggacttttc
     1021 cgtgttcgct gcttcactca ggacgacgct catattctcc ttgctcccga gcagatccgt
     1081 gacgaggttg taaggattgc acagctcttt gatgaggttt acaccctttt cggtcttccg
     1141 tatgaaatcg agctttcaac aatgcccgag gatcatatcg gttcggaaga ggattgggag
     1201 attgcaacga aggcactcgc agacgctatt acaagcgtcg gcaagactta tataatcaac
     1261 gagggcgacg gtgctttcta cggcccgaag cttgacttcc acatcaagga cagcctcgga
     1321 agaacatggc agtgcggtac aattcagctt gactaccagc ttcctggcag attcaatctt
     1381 gaatatacag gctcagacgg agagaagcat acccctgtta tgatccaccg tgttgtattc
     1441 ggttcaatcg agcgtttcat cggagttatc accgagcatt tcgcaggtgc attcccgatt
     1501 tggcttgctc ccgttcaggt taagctcctc ccgattgcag accgtcatca tgactatgcg
     1561 tttgaaattg caaagaagct cgaagccaag ggcattcgct gcgaagttga tacaagaagc
     1621 gaaaagacag gctacaagat tcgtgaagct cagctccaga agattccgta tatgctcctc
     1681 atgggcgaca aggatatcga gggcaactgc gtttcggttc gtaagcgcgg cgagggcgat
     1741 atcggcgcaa tgggcgttga cgaattcgtt gaaatggctg caaagcagat tgaatccaag
     1801 gagattttct gattatgtcc atagaaaaag gcaaggcgta cctcagccgt ttcggcattg
     1861 aggacagggt gagagagttt gacgtgtcaa gcgcaacggt cgagcttgcg gctcaggcgc
     1921 tcggcgttaa tggtcagcga attgcaaaaa gtctctcgtt caaggtcggc gatgaagcga
     1981 tacttgttat cgccgccggt gatgccaagg ttgataacaa aaagtataag gcaactttct
     2041 cgacaaaggc gaagatgcta acttcagatg aggttgaaag catgataggc cacaaaatcg
     2101 gcggtgtttg tccgttcggc ataaatgacg gtgttaatac gtatcttgat gaatcgctga
     2161 agagatttga tacggttttc cccgccgtcg gcagctcgaa cagtgcagtt gagctgacac
     2221 ctgacgagct tgaaaaatat gctcagggtt tcagcggctg ggtcgatgtt tgcaaggttg
     2281 ttgaggaata attaggtcga tgttttgagg atttttcttt gaaaaaatat tgtttcagga
     2341 ataaacctat tagcctcccc ttatggtaag ggtagcgaag tgtcgacacg gtagtgaatg
     2401 acagtccggt ggactgtcag agccgtggcg tgaccgagcc gcagcgagac gggggaccgc
     2461 ttgcggtgga agggataagc attgatgtgt taggaatagg tctttatccc ctccgtcgct
     2521 catgcgacac ctccccttga ttcaaccaag gggaggctga gaacaaataa gttaccgaaa
     2581 aaccttcggg ctgcatttta aaatgtatca taaaccctaa aacatgaaag gaatgattat
     2641 aaaatggcaa acgtaacatt gaaaggcggc gtcgttaagg agtatgaaaa cggcacaacc
     2701 gctatggaaa tcgctaagag cataggcatg ggtctttaca aggctgtttg tnnnnnnnnn
     2761 ncaaggggag gctcaggaaa attgagttta cagaattgtt tataaaatat actcacaagg
     2821 caggtgctat gcatgaaata tgttgttgta ttatatgacg gaatggcgga ttatcccgtt
     2881 cccgctctcg gcggaaaaac gccgatgatg gttgcgaaca agccgaactt tgaccgtctc
     2941 gctcagcgcg gcgaggtcgg acttgtaagg acggttgccg aggggctgaa gcccggcagc
     3001 gatgttgcaa acctcagcgt tatgggatat gatccgaaaa aatattacac gggacgttct
     3061 ccccttgagg ctgtcagcat cggagttaag atggcagacg acgatattgc gctcagatgc
     3121 aatgtcgtaa cgctttccga cgagccgaac tacgaggaca agacgatgat cgactactcg
     3181 gcaggcgata tatcaagtgc cgaagcagcc gagattatca agacggttca ggagcatttc
     3241 ggcggcggtg aatttgattt ttaccacggc gtaagctacc gtcactgcct tattcatcac
     3301 ggcggcacgg tcgatctcgg caacatgact ccgccgcatg atatttccga cagagttgta
     3361 ggtccgtacc tttcaacgtc ggaaaatgcg aagccgcttg ttgcaatgat gaaagaaagc
     3421 tacgatttgc tcaaggatca tcccgtaaac aaaaagagaa ttgccgaggg caagcgtccg
     3481 gcaaattcga tttggctttg gggcgagggc aaaaagccca ttcttccgaa gtttgaggat
     3541 ctctatcata aaaaaggcac ggttatttcc gctgttgacc ttctcaaggg cataggaatt
     3601 tcagcgggca tgaacacgcc agaggttgaa ggagcaacgg gctatatcga cacgaacttt
     3661 gaaggcaagg caaatgccgc cgttgaagat ctcaaaaacg gcgttgactt tgcttatatt
     3721 cattttgaag ctcccgacga gtgcggtcac agaggcgagc ctgagaacaa ggtcaagtcg
     3781 attgaaatga ttgacacccg tgtgcttccg atcattatta agggtct
//
LOCUS       DAWBSW010000190         3826 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4017_length_3826_cov_2.834527, whole genome shotgun sequence.
ACCESSION   DAWBSW010000190 DAWBSW010000000
VERSION     DAWBSW010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4017_length_3826_cov_2.834527"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1847..1856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1116 a          823 c          806 g         1071 t
ORIGIN      
        1 ttcttattcc gccgtcaagc gtgataaacc cgtcggcaat gttatccgtc agactgtgta
       61 cggaatatcc gcacaggcga ataaagattt cttcaatttc ggttctcgaa aatgaaaaaa
      121 gatccgacga ataaaaaccg gtgagccgtc ccgattttgt aatataatca gtccgtccgg
      181 aaaaaacaag ggcaactggt cggtcggctc tcagtcgtat ttcgcaaaga gaatccgtat
      241 ctttttccgc aagagaagta aaaattgagg ggagatattt cagaacaatg tcaatctttt
      301 cttttttgtc gtgcctcatt tttatcacca tttatttttt atgacttctt gtcccgaaat
      361 agaacatatc agatttaaaa taagtgtgaa ttttcagccg tgcttattga aagaccggca
      421 gttgtgtggt aatatataaa ggaaaattat tttgaaaagt gtgtcatgct ttcacataaa
      481 agagaggaat gagtctgttg aaagaaaaaa agagtttttt gaaaaagcac ccgaattcaa
      541 agcttgcaaa atttcttgat cgcaacattt atataatact tgcgtttgtt tgcagtgccg
      601 tacttatgct gttggtatat tattgctttg atattattcc gttcggcaac aaaaccgttc
      661 tgagaatgga tctataccac cagtacggtc cgctttttgc tgagctttat gacagactga
      721 cgcagttcaa gagtatgctt tactcatgga atacgggcgg cggcggagcc ttcctcggca
      781 actattataa ctatctttcc agcccgatcg gcggcttgat agttttactt ttcggtcatg
      841 agaatgtccc cgaagcaatc ggtgcaatgg tatacgtcaa gaatgccatt gccgcggcgg
      901 caatggccta ttatcttaaa aaatcgaacc ataaaaacga tttcacaatc tccgctttcg
      961 gtattctcta tgcattctgc ggcttcttta tcgcctatta ctggaatatt atgtggatcg
     1021 acgcaatgta cctgctcccg cttgttgttc tcggaattga gcgaataatc aataagcaaa
     1081 aggcaacctt ctatattgtt actcttgcaa tcacattctt ctctaactac tacatggcat
     1141 acatgatttg cattttcgcc gtgctctatt tcctcgttta tttcttctca aattattcga
     1201 tcaaggaggt tcttcccccc aagccgatct acgttgatga ggacggtgag atttataacc
     1261 gaaaagtaga caacgtcctt tacagccgat ttgtcgaatc gggtctgctg ttcgctctcg
     1321 gctcgttcgt ggctgtcgga cttgcagccg ttgcgcttct tccgacatat ttctgtctca
     1381 aagcctgctc ggcaacatcg ggcacatttc caagcgatta tcagttctac aataatattt
     1441 ttgatttcct tgcaaatcat cttacctcgc ttgagccgac gatcagaagc agcggcgaag
     1501 acgtccttcc caatgtttat tgcggaatca ttaccgttat gcttgttccg ctctatattt
     1561 tctgcgataa aatcaaagca aaagagcgtg cggcacacat cgttttgctt gctgttttct
     1621 tcctcgggtt caaccttaat tatcttaact atatcgttca tgctttccat ttcccgaacg
     1681 atcttccgtt cagattctca ttcatatatt catttgtttt gctttcaatc gcatacaaag
     1741 caattgtcaa catcaaatcg ctttcaacaa aggccttgct cggctccggc gttgcgacag
     1801 ttctctttgc cgtccttgtt caaaggcgcg gttcaaagaa tgttacnnnn nnnnnnatct
     1861 atctcagcat agcgttcgtt gcggtctata ccgttgttct cggccttatg aaaaagaaaa
     1921 actatgctca gtcggctgtc tcgctcctgc ttatgtgctg cgtctttgcc gaggttgcgg
     1981 tcagcgacac aagccacttt gtcatgagtc agcagaagcc gaactttgtt aacggctata
     2041 ccgagtttag ggagctcaaa gaaaaactcg acaccgttga gggtacggac aaatacagaa
     2101 tggagcttac gaatatcaat acgcttatgg acgcttcatg gttcaactac aacggcgttt
     2161 ccgttttctc atcaatggct tatgaaaaat ccgctaatct ccagcataat ctcggactga
     2221 acagcaacta tatcaacagc tatatttatg ctctgcagac gcctgtttac aatgctatga
     2281 tgtcgctcaa atatcttgtg aataacgacg attcgataat gaacgaccgg ctgtttgaga
     2341 aggttgcgga aaacgacaga tttgttgcat acagaaataa ttactgtctt ccgatcgcat
     2401 acagtgtaag cgaagaagtc gaaaaatgga taacaacggg ttcaaatccg tttgaaatcc
     2461 agaatgatta ctggacaaga gcaaccgatg ccggcgatgt attcaagagg atttcggcaa
     2521 cgacctacag caccgaaaat atcagaagcg acgatgaact ccttacggag aatttctcgt
     2581 ataataaaga aataacgggt caggaaggct caatagtcat caaatatgaa attcccgaat
     2641 ctcaaaacgt ctatctctac accgactgca agagtgcaga tacaataaac gtttcgggca
     2701 caaacggttt ttcaaaatct caggatccga gcgatccgta tgttctcgac tgcggttatc
     2761 ttgaaaaggg cgaaattctt acccttgaaa tcaaaatccc gtcggacaag gagtccggct
     2821 atgccgatat ttatgccgta ggtcttgata tgaataattt tgaagccggt tacaacaagc
     2881 ttgccgcttc ggcgatggat atttccgagt tcacggacac ccacatcaag ggaaccgtta
     2941 acgtcaaggc aaatgagctt gtttacacct caattaacta tgatgaaggc tgggagataa
     3001 ccgttgacgg cgaaaaggtt gagccggtcg ctctcggcga tgcacttatc ggaattgatg
     3061 ccgctcccgg aacacacaca attgaaatga cctataagcc gaagggcttt atgataggag
     3121 cagttgtttc cgcaggcacc ttcgttgcat ttattgttgc tcttattata attcacatcg
     3181 taaagaaaaa gagatatgca aaaagcattg agatcgacag caaggaaaat gttgaaactt
     3241 cgacggacgg accgacaggc attgacgcac tgatggagca ggatctcggt aaaaatgcaa
     3301 ccgttgagga cgttgaaaaa ctgcttgacg ttcctgacga atatgagatg aataagcagg
     3361 atccgaccga aactctcaac agagcggacg agattcttaa cacacagaaa ataaatttaa
     3421 aagaaattct atcccagata gagggtaacg acgactcgga ggacaaggaa tgaacagaac
     3481 aataattacg gtcataggca aggatagagt gggcataatc gcaaaaattt gtacctatct
     3541 cgctgagaat gacatcaatg tactcgacat taaccagtca attgttcagg gctttttcaa
     3601 catgataatg atcgttgacg ctcagaatgc taagaaagac ttcgccgttc tcaacggtga
     3661 aattgaaaaa atcagcgaag agctcggtgt aaaaattaag atgcagcacg aggatatttt
     3721 caacgtaatg cacagaattt gaggtaggct atgataaact cctttgaagt tcgtgaaacg
     3781 aacaaaatga ttgaagaaaa taacctcgac gtgcgtacga tcacca
//
LOCUS       DAWBSW010000191         3816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4026_length_3816_cov_2.526190, whole genome shotgun sequence.
ACCESSION   DAWBSW010000191 DAWBSW010000000
VERSION     DAWBSW010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_3816_cov_2.526190"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    698..707
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1606..1615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1105 a          740 c          830 g         1121 t
ORIGIN      
        1 attgcgctgt tgacatagct ctgcgaataa tcaatcgagc tgagtgattg gcaaagaata
       61 tccttccatt caataagtga attttcttcg caggtgattg ccgcaatgtt ataagccata
      121 taatagccgg cgtcaattcc gaagccactc atcatattgc ctgcgaaatc tattatatcc
      181 gccgtgaaaa ggccctctcc cttggtgccg gctgcatcgg tgaaagtagc acggagcagg
      241 gcgggactga ttgcctgcga tttcatgttg ctgttactcg caaagcgttc aacgctcttg
      301 aacgacccga agctcggaaa gtcgaagctt gaataagttg gttcatatgt cttgacaaga
      361 gagacatatg aattaaaaat cttaaagaag ttttccgtcg acggagagga gagaaccggt
      421 gcgtcagcca tcatgttata gttcccgccg tacatttgat atgactgccg ataataatcc
      481 ttacccgtct cactgtgaag gagcggttga gccttgagca ggaaaaacac ctgatttatg
      541 gggttcttgg ggtcggttac acggattgca tggaacattc cgattcctcc ggcagtaaca
      601 gaccaaccct ccggaatttt cattgaaaag tcggatgttt tgtagtcgac aagctttgct
      661 tgcttcgcct ctgacggggt gatttttaca tcgctgannn nnnnnnnccg gaaggcaagg
      721 acttgtcgtc ggcctccttt ttgccgcagg cggaaagcga aaaaatcatc aaagccgcca
      781 gccaaagcgc agcgattctc tttttcatat gtatcatatc tcccttcgac ctgtattatg
      841 aaatcattat acttgaaatg aaaataaaaa gcaatacaca ttatttcttt tttataattt
      901 ttacttgcag taaaactccc cgaagtcgcc ataaaacgat tcggggagtt gtgtgttttt
      961 tattgtcttt ttcttcctct ttggttttgt tgatgtaata aacaataaat gcggcggcag
     1021 cggcgattgc caaaacagag ctgaaaactg caccgttaac acctaaaagg tcgtcaatgc
     1081 tgaacccgat aaagccaaat agcaaggtta caaaagccac aagcaaagcc gttttaaaag
     1141 ctttattcat tatatattat ccctctctaa atacattatt atttgctaat tccataataa
     1201 tataactggg aaaacttgtc aatcatagat gaagtatggt ataataaaaa aataatctat
     1261 ttaaaactat tatagataaa aaagcaaatg gaggttttag aatggatttg aaaacgtata
     1321 ccgaactatt tagcgactgc ggcgttaatg tttcctgcgt cgaacagttt ttagggttca
     1381 agcattatat caattattat aaactaaaca accataagga ctggatgatg cttgaccgag
     1441 caaaaaagcg gattataaaa tatactggca gcgctgagtt ttacggagag ttagaaagtg
     1501 acgccgattt tgcattgatt tttacgcttc cgcctgaaga atggttcacg attaaatacg
     1561 agaatacgcc agtaaacgca caatcaaaaa gcggcgattt tttaannnnn nnnnncgcta
     1621 atgaattgta tcattgacag cttgcatcgg tcaacctcaa atttgatttt ggactttatc
     1681 gacttgaaac aagttgaatt cagcttttat aattatttcc ctgaaacgca atataaagta
     1741 tcaacgacaa aaagagaagc ggcgctattg cttgaagatg tgaaagagga aatacaaaaa
     1801 cgatatgcag aaatggaaag taaaggactg caaaaagcaa ccgccgagga tttcccatta
     1861 cacgtcattt ttattgacga atacgcaacg cttgacgccg cagcaggtac acggattaac
     1921 tcccttgtta gctatataac cgccgtaggc agggcgtgta atgtgtttat ggtcattgca
     1981 acgcaacacc caaaaaacaa tgtgattgat aatacaattc gggcaaactg tttatcaaaa
     2041 attgttttag cgtgtgcaaa cgtggcacaa agcaatacca tatgcggaca gaaaatcggc
     2101 gttgagctac tcggcaaggg cgacgggtac gcaattctcg acggagtggt taagcccata
     2161 cggtttcaag ctccgctata cgatgacgag acgagaaaag cactttataa aatagtacct
     2221 taattaaaat gagcgactga ggaatcagcc gcttattttt ttactatgtt taaaatattc
     2281 aatgtgagcc gatataagcg actttgcttt gagtagttta tttaaataaa aaagcagaga
     2341 agcaaaactc gcttctctgt cttggtggag acggacggga tcgaaccgtt gacctcttga
     2401 atgccattca caaaaataaa aggtaaaaaa cgttgatatt acgtcgtttg tggctttttt
     2461 gactgacaca atgactgaca tcaaccttga ttgtcgagaa aactgttaag tttatcgata
     2521 tttttattct tgaattgcga atcaagatga gtgtaaatat caagtgtggt ttgtattttt
     2581 gagtgcccga gcagctcttt tgcggtaagc acatcaacac cggcatggta taggatcgta
     2641 gcgtatgtgt gtcgcaacca gtgcggtgta aagtcaggca tcagcggatt agcttcctgc
     2701 aaagcacaga tataagactg ccacattctc cgccatgtag ttaagctcat atatgtacct
     2761 ttttgggtgc agcatacaat atcagacttg gctttggtct tacactcttt taaatacccg
     2821 gagagggcag aggggatatt tacaattcta actgagcttt tagttttggc gccatcttta
     2881 atgttgatct gattagttat gatttcggca cttttattta ttgatataat acgattgtca
     2941 aggtcaatat cattccacgt taaggcgagg agctcgccgc gccttaaacc ggcatacatc
     3001 attatcattg ccgataattt acacgggtgt tcaacggcaa gaattgtttt gcgctcggat
     3061 tcgttcaagg ctcgtctgtt ttgctttgta ttatctttgg gcaggaaaac agatgttgca
     3121 aaattaaagt ccgtcaaacg tgcggagatt gcataatttg caacctgcaa agcggttgat
     3181 tttattgcga cgagcgtccg gtgactgctc ggttttcttg tgttggggtt aaattttgca
     3241 tatcggtcaa tgatatcctg taaatcgctc ggaacaagcc tgcgcatatt cttatcaccg
     3301 atgtatcggt tgaggtgatt aagacaagat ttgtactgca cgacctgaga acggcatacg
     3361 gcgttttctt ttgttttaag ccagcgttcc gcaatttcgg aaaaagttga tgtctcggat
     3421 atgtcaagcc ctttttccaa agctcgacgc acttcatcgg cttttcgacc atcggatcgg
     3481 gttgcatttg actttcttgg cataaaaata accgtccttt caaaaaatgg ttgcaaaaac
     3541 ctgagaaagg cggtataatg ttgccacagc ctttcttttg gtggttcgag agattggatg
     3601 ttttgccgtt cggtgttcca gcgccgggcg gcgtttttta cttctattga tgcgaaaata
     3661 aatcacaaat cagtctttcg ggtaggtgtc gaaggtttca tttgttgaaa tgtcgattat
     3721 attatcttac ttatctaaaa tgacttgcgc tgcggtggtg gtagtgcttt tacccgaact
     3781 atccgggctt gaatcattgg taacgctgat aatgag
//
LOCUS       DAWBSW010000192         3797 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4042_length_3797_cov_2.577766, whole genome shotgun sequence.
ACCESSION   DAWBSW010000192 DAWBSW010000000
VERSION     DAWBSW010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_3797_cov_2.577766"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1085 a          829 c          863 g         1020 t
ORIGIN      
        1 gccgtatgac tgaaaggaag aagaaaactt tgacgatagt cggaactgtc gtgcttgcgg
       61 ttgcagttgc atatactttt cgtctcatcg gaaacggcaa ttattatttt gttctgttcc
      121 cctatctgcg cagctttatt tatatcggtc tttttatcgc gtggggtatt tccatacgcc
      181 gccgtattgt tcaaaaacag gttgcgcaat atttgacggg catatccgta cttcttattc
      241 tgtggttcac tttccgctgc gcaaaatatt ttattttttg gcagccgacc gctataagat
      301 atctatggta tctttactat ttaccaatgc tttttattcc gatacttgct ctgctcgttg
      361 cactgtctct cggaaaacct gatgattacc gtctgccgaa agcggcttgc gcactgtgga
      421 taatctccgg aatattgctt ctcctggtgc taacgaatga cctgcatcag cttgttttca
      481 cttttcccga ggatgcggaa gtgtggtccg acaaatccaa cggatacggc atcggcagct
      541 ttattgtgat cggctggcag atcttttgca tgatatcggc actcgttgtt atgattataa
      601 aatgcagact gaaaaacggc agacgtcaac tgtggacggt aatacctatg gttgtgtcac
      661 ttgtctatct tggccttaat tatatggatg tgcaatggtt taaattgttt ttcggtgata
      721 taaccgcctt ccaaagcctt atgtatatgc tctgttttga aggctgcatc acctgcggct
      781 ttatccactc aaacagccgt cattttgact tgttctcgtc ctcgatcggc acatctgcgg
      841 tgataaccga taaggatttc aatgttcggt atgccgcact gaacgcacgt cggattccga
      901 gaatagatat gataaaggcg gagcaaagcc ccctgacggt tgaaaacggc ttgacggttc
      961 attccattcc cgtgaacgga ggatatgccg tttggacaga ggatatgtct gccctgctca
     1021 aaatcaaaga gcaatccgaa agccttgccg aggaattgaa cgaacgtaac aatctgctgc
     1081 gttatgaata taaacgtgag gcaaaaagac aaaagattga ggaacaaaac cgattgtatg
     1141 atttgcttcg cagtgcaact caaactcaaa tcaacaagat ctcggtgctt acaaaggaat
     1201 atcaaaaaat aaacaaaaca gatccgacaa gggcaaaagc tttacttgcc gaaattgccg
     1261 ttctttgcag ctatatcaag cgtcgtaaac atttaacgtt gttgaccgac cgtgactata
     1321 aggttgcgat aaccgaactg gtaagagcct tcaccgagtc gcttcaaacg ctgaagctgc
     1381 taaacgtgcg cagcacattc tttgccgaca gcgagctttc catgctcccg ggaaaatcgg
     1441 ctgcggcgat atttgatttt tacgaacagg tgattgaatc ggaccttgaa aacatatccg
     1501 gcatacaagt aagtctttct gatgttgaag ggctgcgttt atcaataaat atatgctgca
     1561 agaccgacct gtcctcactt gcgtgcaagg gtaacgtacg gtatgaaacg gacggtgacg
     1621 aaggatatca gcatcttgta ttccttctcg aaggaggtgt cgccgtatga gtgccttttc
     1681 ttcgctcccg gactttttgc aaaccttaat accgcttctg cttttcttgg aagccgtgct
     1741 tgaattggga ctttttattt atcaattctc tcaccgctcc aaatcgctcg gcaatttacc
     1801 aagtctcgtt atttttgcat ttttgcttac acttttactc tcggtgactc agggagaccc
     1861 gtttaaaggc aaggatgcgt tcttattaga tgctccttgg cagattttca ttgcggtaat
     1921 tttacttgtt attgttcatt tcgcctttgc cctgccgaga gagtaccgcc gccgaaaaaa
     1981 tatgctctcc ccgttctccg ttaaagacgc caccgacaag ctgcccatgg gtttatgcta
     2041 tgccgacccg aacggacgta ttattctttg caatgaccgt atgcgccgtc tgtcgtttgc
     2101 gctgagcggc catgagcttc aaatcaccga agatcttgaa aaagcgctgg agcaacccga
     2161 caaaagcatt accgttaacg gcgactgtta cattctgccc gataaaacag tttggcaatt
     2221 ccgaaagcag aacatcaccg ttgacaatga cagcaactgg cttcaaatga cagcacataa
     2281 cgtcactgag ctttataacg gcaacgttcg tcaagccgtt ataaacgacg agctgaagga
     2341 ggtcaaccgc aagcttcaaa aaatgtatga acgaatggcc gacgatataa aagaaaaaga
     2401 aagtcttgac ctgaaaatac atatacatga cacaatcgga agaagtttgc tgacgattcg
     2461 tgacataatc gaaagcgatg aaaacaccga taaaaagctt gaagctttac aagaagccac
     2521 cgcagtgctg acaagcgacc gtgtaacagc cggcggaaca atggacgagg ttattcaaac
     2581 gtcgaaaatt ctcggcgtaa cggtaacggt gaacggatac tttcccgccg attctcttgc
     2641 cgaagagctt accgttgccg cggcgaaaga atgtgtaaca aactgtatca ggcatgccgg
     2701 cggcaatgaa gtatatatct gcatatttga gcgaaacgac ttgtttgata ttacgataac
     2761 gaacaacggc gctgccccgt cggagcccat taaggaggga agcggacttt cttcgcttcg
     2821 ccgcagcata gaatcctccg gcggcgaaat gcacacggca tacaagccac gcttcgcatt
     2881 actgcttact cttccggaaa aggagaacaa catatgataa atacaatact ggttgaagat
     2941 gatttatata ttcaaaagca ttttgccgag cggctgaaat caaacgaagt ttttcgcctt
     3001 gtcggcgtat atcgtgacgc ttttgaggcg gagaaaaact gcaacggatc ggtacagctt
     3061 attcttatgg atgtacaaac tcagcacaag cactccggcc tcgccgccgc agagcgaatt
     3121 aagaaggttt ttcctgatat aaaaatagtg gcagtcacgt ccttggtcga tccggaagtc
     3181 ctttccaagg cgaaaaacgg tgccgccgac agcctgtggt ataaggacca cggaacggag
     3241 gagcttttgg atgtaataaa aagaaccctt gacggagaaa aagttttccc ttcctcatct
     3301 ccgtcggtcg aaatggaaaa agccatgtca aaagactttt cgccacgtca gcttgacatt
     3361 ttgcgtttgt atattcgcgg actgacatat caggaaatcg ccgataaaat cggaatatcg
     3421 aaaaacggtg tgcgatggaa tctggacgac atggtggaaa aaggtgaatt tgaaagccgt
     3481 gaagcgctgg ttgccactgc aattgaaaac aagctgatgg tcacaacatt aaaagatgaa
     3541 taatataaaa acatatatcc tcggtttcaa aaaccgagga tttttttgtg ctgtttgaca
     3601 gatatttttg aaaaacaatt attttttgta cgtttactgg cacaaatgcc agtgacaaac
     3661 gggaaaaaat aagtacaatt ataagtgaaa aagaaaatga ataataaggc ggtggtggga
     3721 atgagaaaaa ttgtattgga tattcaaagt actattcatg cacacaatat ggaaagaatg
     3781 cttatgcagg aattgga
//
LOCUS       DAWBSW010000193         3788 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4049_length_3788_cov_2.487276, whole genome shotgun sequence.
ACCESSION   DAWBSW010000193 DAWBSW010000000
VERSION     DAWBSW010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_3788_cov_2.487276"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    477..486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1249..1258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1117 a          780 c          829 g         1042 t
ORIGIN      
        1 attgtcaaca acgggtttgt aacaaagccg tatgaagtca gagtttatat gtttaaataa
       61 ggtgaaacaa tatgaaaaat gcaattaaag ttctttgctt tacgcttgcc gtaattattg
      121 tcggcacgtc ggcgtttctg atttatttca aatgggataa gtcgcaattt gaatatgttg
      181 acggcacgga aaaaggcacg gtaatgatta caggatacaa ggacgaaagc aaagatatta
      241 caatcccttc aaagctcaga ggaaaaaaag ttacgcagat tgacacctcg gcttttgagg
      301 agagcgatat tacctcgatt gagataggtg ataatgtgac ctatatcgga aagagcgctt
      361 tcaggggctg caaatcactt aaaaccgtta agcttggaaa atcaatactt aatattgacg
      421 agggcagctt taacgattgt acgtcgcttg aagaaatcaa gctcccgaaa agccttnnnn
      481 nnnnnncttg aaaagctcgg cggaagtgtt tttgccggat gtacgtcgct taaaaatgtt
      541 gagcttgagg acggcggaaa ctttgtcatc aaggacggtg ttctcctctc ggcggatatg
      601 aaaacacttt attttgcatt gccttatgca aagctcggca attatacctg cccgagcgag
      661 gtcgaaacaa tcaattcgct tgctttttat aactgcccca cgcttacagg cttcaatttt
      721 tcaaataagg tgaagtcaat tccgcagggt atgtttgtac tttgtaccgg acttaaagaa
      781 ctgcatcttc cggattccgt tactcagatc ggttcggtta ttattacaaa atccggagtc
      841 aaaaccgtta caattcccga atccgttaag gttattgaca aaagcgcttt tatacagtcc
      901 gacggcacaa acagcaagga tcttgtcctt cgagtaagag caggctcgaa agccgaaagc
      961 tttgcaaagg aaaacagtat taaaatcgaa tacataaaat aaaaaagctg ccgcattggg
     1021 ataaacctga atgcggcagt tattttttta atttttatag ttcggaacaa atttttcgcg
     1081 aagaaaaaat gttgatgtct cgcaccggaa ctaacgaatt ttcatatgac cttgggtcga
     1141 tgtggtcatc gacctctacg aattgtggct ttgttatagc aaattgtagg gtgcgatgcc
     1201 cacatcgcac caaaaagatt tcagcttaat ctgtcaaatt ttctttatnn nnnnnnnncc
     1261 cctccgtcgt caatgacgcc acctcccctt gcaacaaggg gaggcttgga taatttctgc
     1321 tgactttatg attgaatcat tatatttacg ttctaatctt gggtcgatgt ggtcatagac
     1381 ccctacaaaa aagctcttaa atcatatctt atgtgctgac tgaatgttac tcaataatct
     1441 ccgcgctcca cgggaatacg aattcttcac cgggtttaag cttctgaatg agacgtttct
     1501 gtgagaaatc gtccttaacc tctcttgaat cgtttattcc gtaccagggt tcgatgcaga
     1561 catattctgc gcccggcttt gcccatattc cgaggacggg agctttgccg aagttgaatt
     1621 taattttttg aggacgcaaa tcgctctcga ggtaaaccgt atccgacttc attcccgaaa
     1681 gaatgagtgc gtcgccgtca aaaatgtcct ttgtgattac aatatctgtt tcattattca
     1741 acgtcggata atgcttgccg tcgagaattg aatctgtatc aattttttcg caaacaagag
     1801 tctcaggctc tgcaaatgac aatctgtcgc cgattttaca attgaatccc gggtgagcgc
     1861 cgatcgaaaa atacataatt ttatcgtcgg catttttaat tgtgtgggtt actgtcaatt
     1921 tcttgccctc aagcttaaat tcaatgtcga aaatatattt gaacggataa cgttcaaggg
     1981 attcgtcatt ccaactttgg gtaagcgtga ggtgatcctc gccctgctct ttaagctcaa
     2041 aatctctttt tcttgcaatt ccgtggcgga acatctcata ttccttgccg tcgattctgc
     2101 acttgttgtc aagcaatgta cctacaatcg ggaagagaat aggggactga ccgtaccaaa
     2161 cgtcgggatt gccctgccaa aggtactcga ttgatgtgtc aagcgatttg aacgaggaaa
     2221 gctctgcacc gtatttttta accgaaatgc cgatcgaatt attatttaaa gtatagttca
     2281 taatgccact ccttcatttg ttttatcaat tatatacaat ctatcaaaaa aaatcaataa
     2341 agttattgat atttgtagcg aaaaatggta ttatatacaa gatattgggc tattatcgct
     2401 taatatatat ttttatatat ttaatatatt acggagggta acatggcggc aaataataat
     2461 aacaagaaaa agaatacgtc gtcagccaag aagaaaacag cttcttcaaa acagaagaat
     2521 actttgcagc aaaagaaagg cccggcaccg actcagcctg tcagaaaaca gactaaagcg
     2581 cctgccgaaa acaaaaaagc ctcgtcgaag aacgagaaca gagtttcggc ggcggttatc
     2641 cgtcagcgcg cggcaataat tctctctgct gtgacggcaa ttcttcttgc ggcaacattt
     2701 ataaaaggcg aaagggtatg gcttgcattt cacgaattta ttttcgggat tgcgggcatc
     2761 tgcgcttatg tttggccgct gatgctgatt tatataatcg ttatgcttgc ttcggataag
     2821 agcgcaaatg caattaagtc gctcagaggc aggcttatcg gtgcatcact cgcaacggga
     2881 atgctttcgg gtattgtcca cgttttctcc gatgctgtcg attcgggcaa tttaaaagat
     2941 caaatcgaat atgtttggaa tttgaaggac accgtgacag taggaagcgg cggcattata
     3001 ggcgcgattt tcggcggatt accggaaatg cttttcggca aaaccgctgc cgcaattgtg
     3061 cttattatcc ttcttatagc aacgcttatg tttgtaacgg gcagcacact tttgggtgtg
     3121 tatattttca ttaaggaaag aattgattcc gcaaaagtaa aacagcagga gagggttgaa
     3181 gcggacgact tcgacgacag cgcttcgttt atcagaaaga aaaagaaata tgttcctgtc
     3241 gatattggcc ccgaggcata cggttctccc gaggacgacg gaatgtatat agaggagctt
     3301 gtcggaaacg ccgagaaaaa ggacattacg ccgagaagca agtctccgaa gccctttgtc
     3361 gatgttaagc ccgaaccgga aatttccgct gcggatcccg ctttgccgct tgacgagata
     3421 attaaaaagg tcaacagctc cgtcaagcct aaggacaagc ctgccgaaaa agaaaagaca
     3481 gaacctaaaa aggatacaaa ggcaggaaaa accgacgaac ccgttcaggt gttccatacc
     3541 tatcaaaagc ctccgatcga gtgcctgaaa tatgcaaata atgcaggtgc aatgaactat
     3601 gaggaggagc tgaaaaacaa cgcaactaag ctggttgaaa cgctcaacag cttcggagtt
     3661 tcgacaaaaa tcgtcgatat ctgccgcggt ccgtccgtta cacgttatga attacagccg
     3721 gcggcaggcg ttaaaatcag caagatcaca agtcttgccg acgatatagc ccttgctctt
     3781 gcttcgtc
//
LOCUS       DAWBSW010000194         3750 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4098_length_3750_cov_2.574560, whole genome shotgun sequence.
ACCESSION   DAWBSW010000194 DAWBSW010000000
VERSION     DAWBSW010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_3750_cov_2.574560"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1936..1945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1088 a          763 c          848 g         1041 t
ORIGIN      
        1 caggagcggc aggcggactc ggctttggac tgataagctt tctcggtgca aggcttgtca
       61 agggcgcgcc ggcggtgctt aaagcgacag gctatgaaaa tgccacaaag aatgccgatc
      121 ttgtaatcac gggcgagggc tgtatggaca accagtccct gcttggaaaa gcgccggccg
      181 aggttgcttc actttcggga gatacacccg ttgttgcaat cgtcgggatg agcagagtca
      241 cggatatgag cggctctaat atccgcagaa tttacgtcac cgaccacggt caaagacctt
      301 ttgagcagat tctgaaagag tgccgcaatg accttgcagc ggcggcgaac agagttgcat
      361 atgattttta caacggtatg atttaaaagt tttcaacatt ttccacagaa ttttccacac
      421 tttttgttga aattcccgtt ttatgatttc gttttagaac attaaaggcg ttttaaagaa
      481 ttttcaacag gatatattat taaaaataaa aacggcaaca gggagacgaa taaatgacaa
      541 aaaagagaaa cggcaatata gaaattctaa gattcatttt ttgcatgctt attatactcg
      601 tgcattttcc gcaggaagtc agatacttct tcggagcttc cggatattta ggtgtagagt
      661 ttttcttcat gatttccgga ctctttcttg gcaggaagct aaaaaaggac aaagaattac
      721 atcaaaaaga acctatatcg gatactttaa catcttcatg gaaatatttt ctttcaagaa
      781 taaagtctat ttatccgttt tatttcgctg cagtcggttt cttctgcatt ttaaacatta
      841 tactaaagta taccaaatta ttaaatagca ttttatactg catcagcgac cttctttttc
      901 tgcaaatttt tagttttccg agcatttcat ataccggaac tttatggttt ttggcagcat
      961 tgtttttcag tctgtttgtt ttatacccta ttgtcagaag atactatgat gtttacataa
     1021 aatatttttc tcttccgatt gttcttgtga atatggcata tgtcatgcga atttttcatt
     1081 cctttgaagc cgaacctttt tttgacagct ttcttgtcaa tccgggaatt tttcgagcaa
     1141 tagcgatgct cactctcggg atgcttgtag gagagctttc ggataaattg aaaaccctga
     1201 cgctctcaag gtcaggtaaa gtgattttga caacagcgga gctgattagc tatgttttcg
     1261 cctttgcctt tatgattgcg tgcagaagcg atcccgtaaa acgctttgat tatgcggcag
     1321 tgatacttat ttttatcggc ctggtgatta ccgtcagtga acattcctat tttaacggaa
     1381 aatttgataa tcgtttttct ttgttcctcg gaaaaagcag tatgattttg tttatgacgc
     1441 attatttttg ggtacataat ataaaagaat tgactctacg gtttacagga cacgatgata
     1501 taggtaacat tgaattgctt ttaatcggat acgctttatc attcattaca ggtatagcag
     1561 tttattttgt aggtcttgcc ataagaaaag tgttttataa aataaaagat atgattgttt
     1621 taagcgaaaa gcctgcatgt tcgtaaattg aattaaatat agaccgattt tttcaataaa
     1681 cacttgacaa tacaaatcaa ataatgtata atacatgcct gtaaagcaaa aaactttaca
     1741 ggcatttttt tgaaaggacg agtgagatga gtaaaaatct tgacataaca ctgttgcttg
     1801 atttttacgg cgacctgctg accgaaaagc agcgtacctt tatcagctat tactacaatg
     1861 acgatctctc acttgccgaa attgcagcca acgagggcat aacccgtcaa ggagtgcgtg
     1921 atgccataaa gcgagnnnnn nnnnnacaag gtagtgtgcc gaatggcatt tgacggtctt
     1981 tcagaaagac ttgaagccgc ttttcagcga ttgaaaagca aaggctcgtt gaccgaagcg
     2041 gacgtccgca gcgcaatgcg tgaggttcgt ctggctctgc ttgaggcgga cgttaactac
     2101 aaggttgcca aggactttac cgaaaaggtt acaaagagag ccgtcggaga gcaggttatg
     2161 gaaagcctta ctcctgctca gatggttatt aaaattgtca acgaagagct gaccgagctc
     2221 atgggcggaa caaaagcccg tcttgcggtt gccaatcatc ccccgacagt cgttatgatg
     2281 tgcggccttc agggctccgg taaaacaact cacagcgcaa agatagcttt gatgctcaag
     2341 aatgagggtc acaggcccct ccttgccgcc tgcgatattt accgtcctgc cgctatcaag
     2401 cagttgcagg ttgtcggcga aagcatcggc gttcccgttt ttgaaatggg tcagacaaat
     2461 cccgttgaaa ttgccaagga agctattaag ctcgccaagg atcacggata tgactatgtt
     2521 tttcttgaca cggcaggacg tctgcacatt gatgaggagc tcatgaccga gctgaagaac
     2581 atcaaggcag aggtcaagcc gcatgaaatt ctgcttgttg tcgatgcaat gacaggtcag
     2641 gacgcagtca atgtagcgac atcattcaat gatgcgctcg gcattgacgg catcgttctt
     2701 acaaagctcg acggtgatac ccgtggcggt gcggcgcttt caacaagagc cgttacaggc
     2761 aaaccgatta agtttgtcgg cgtaggtgaa aagcttaccg atcttgacac cttccacccg
     2821 gatcgaatgg catcgagaat tctcggcatg ggcgatatgc tttctcttat tgaaaaagct
     2881 gaaatggccc ttgatgagaa aaaggcaatg gagcttgaaa agagactcaa gcaaaacaag
     2941 tttgacatga acgacctgct tgaccagttg gcacagattc gccgcatggg ttctatcaag
     3001 gacactcttt cgcttatccc cggcgtcgag aaacaaatca aagacattga tattgatgaa
     3061 cgccagtttg acagagttcg cgcaataatt gagtccatga ctcctcagga gcgtgaaaag
     3121 cccgagatta tcaatccctc tcgcaaaaag agaattgccg caggttcggg tcaggatgtt
     3181 gaagcggtca acaaacttct cacacagcat aagcagatgc aaaagatgtt taagcagatg
     3241 aacggtaagg gcaaaaaacg cagaagagga cgtcccgacc tttctcagct caagggcttt
     3301 aacatttaag gcaataccgc acaaattgcg gctcatgcct tgtcttaagc tttcaaacac
     3361 gcacttatca cttgcgtgat tgatatatta tttataattt ttatttttgg aggaatttta
     3421 caatggttaa aatcagactt cgccgtatgg gcgcaaagaa agcacctttc taccgtgttg
     3481 ttgttgcgga ttcaaagtat ccccgtgacg gtagattcat cgaggagatc ggcacatatg
     3541 atccgatgaa gaaccctgct gaaatcaaga tcgacgcaga ggcagccgca aagtggcttg
     3601 caaacggcgc acagccgaca gataccgtca aggatcttct caagaagagc ggcatcgtta
     3661 aataatcaaa cgaggtaaca atgatgaatg aattattggt acagatcgca aaaggactcg
     3721 taagcgatcc cgatgcggta aacgtaacag
//
LOCUS       DAWBSW010000195         3661 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4218_length_3661_cov_2.436772, whole genome shotgun sequence.
ACCESSION   DAWBSW010000195 DAWBSW010000000
VERSION     DAWBSW010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_3661_cov_2.436772"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2582..2591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1082 a          750 c          893 g          926 t
ORIGIN      
        1 cactctcaga gaggaaattc ctataaactg gaataccgac acgagagaca agggcgatca
       61 gctcaactat ttcggtgcgg ttaaagcaac ggaatatttc ggcaaatatc ttgaagaaaa
      121 aaggattttc agcgataaaa gaaagctgaa tgaatacaaa gattggaaca gcacggcaga
      181 tgaatttttg tcgtcggttg aaaaggttta atgcaataat tttcgaattt caaacgtgtt
      241 aaaagagaaa ggaaaaaata aggggcagga atatggtaat aaatgtactc gaatggctgg
      301 aaaaatcagc cgaaaaatat aatgataaaa ttgcttattc cgatgaggaa agctcgctga
      361 gctttttgca ggtgaaaagc ctggcgcaaa gcatcggctc ggcactcatt gaaagagggg
      421 cggacggaaa tcctatagcc gttttctcgg gcagaagcgt tatgaccgtt gccgcttttc
      481 tcggcgttgt ttacagcgga tgcagctatg ctccgataga cagcaagctg ccgagagcga
      541 gaatagaaaa aataatggaa acgctctctc cgtcgattat tctgacggac ggcgagaact
      601 gcgatttcgt aaaagagctt ggctttgatg atggaaaaat tgttttgctt gaggacgcaa
      661 tcgggcacaa agcggatgat gaaaagctca aaaaaattcg ccgtttttca aatgaaaacg
      721 atccgcttta tattattttt acctcaggct caacaggaaa tcccaagggt gtgatgacgt
      781 cgcatcattc gctgatgtgc tatatcgaag catatacgtc ggttatgaga attgatgata
      841 aagacattct cggcaatcag tcgccgcttg attatatcgc cgcaatcaga gatatttata
      901 ttccgctttt gtgcggctct tcaacggtga taatcccaaa gatgtgtttt tcctcgccgg
      961 ctaagctttt tgactttatg aacgagaaaa agatcacgtc ggtcggttgg agcgtttctg
     1021 cgctgaccgt tcccgtttcg ctcggtgcct ttgaacacgg cagacccgag tatctcaaaa
     1081 aggtttgctt ctcgggctcg gtcatgccgg ggaaatgcct gaagcagtgg cagaaaaatc
     1141 tgcccgatac actctttgtg aatcagtacg gcccgacgga ggcaaccgct tcgtgcacct
     1201 atcatattgt tacggaaaag gcggaggatg atacggttct tccgataggc ttgccgtata
     1261 aaaactaccg agtatttctt ctcaatgagg acggcacggc tacgaagcag ggcgcggagg
     1321 gtgaaatttg cgtcagcggt ccgattcttg cactcggcta ttacaacgac cccgaaagaa
     1381 cagcggcatc gtttattcag aatccgctta acaaagcgta taacgagctt atctacaaaa
     1441 cgggcgatat cggcgttatg cgtgaggacg gagtgattga attccacggc aggatggaca
     1501 ggcagataaa gcatctcgga caccgcgtcg agctggacga ggtcgagctt gccgcaggca
     1561 gggtggacgg tgttaatgaa tgttccgttg tttataataa agagaaggaa accatttggc
     1621 ttttctacac cggcatggca acggtgaaag aaattgccgt tgagctcaga aaatatcttc
     1681 ccggctttat ggtgccgaga aaaattaaaa atcttgagga aataccaaga ttgccaaacg
     1741 gcaaaattga tataaataca ttaaaggcaa tgtcgaacaa ttaacgcttg tgccttaatt
     1801 gaatgacata gcctaaatct aacaaaaaaa tcggaggaag aaacaatgaa agaagaactt
     1861 atggaaatac ttgaggaact cagacccgac gttgatttcg aaaacgaaaa gcagctcatc
     1921 acggacggaa ttctggagtc gtttgatatc gttgcactcg tcggagaggt aagcgatgct
     1981 tttgatattg agcttcacgt cgaggatctt gttcccgaca acttcaattc gatagaggga
     2041 atgatggagc ttatcgaaaa gctccgggac gaataatatg aggaacgaaa tacttttgaa
     2101 agcggcggaa aaattcggca cgccaagctt tgtctttgat acggacgagt ttgcacgcca
     2161 agccgattcg ataaaatcgg cactcgtaga tattccgctt tgcttctcga taaaagcgaa
     2221 tcccttcctg cttcatgcgc ttcccgagtc cgtgagcagg gttgaggttt gcagtccggg
     2281 tgagttgacg atttgccaaa agctgaacgt taagcccgag acgattattt attcgggcgt
     2341 caataaggga acggaagata tcgcccgtgc cgtcgattac ggcgccggga ttatgaccgc
     2401 cgagagccgg ctgcatgttg agttgatcaa ccgtgtctgc cttgagaggg gcaaaagggc
     2461 agatgttatt cttcgcctga caagcggaaa tcagttcggt atcgaccccg atgaaattat
     2521 cgaaataatc tcaaacaaag aaaattatga agcgattgat tttatcggac ttcattatta
     2581 cnnnnnnnnn ncattattac tccggcacgc agaagaaaaa cgcaggagtt atcgaaaagg
     2641 atattcgcaa gtttgaagag tttatcgaac gccttgaagc tgaatgcggc tatgtgccga
     2701 agcacgttga atacggcccc ggagcagcgg cggattattt cgttccgccc tacgaggaaa
     2761 aggataacga gacactgaaa gagacagccg agcttttgaa aagctttgca aaaaaatatc
     2821 cgctcacagt tgaaatgggc agatttcttg cgtcatcatg cggaacctac cttacgtccg
     2881 ttgccgacat caaaaacaat ctcggcacga attacgtcat ctgcgacggc ggaataaatc
     2941 atctgaaata ttacggacaa accatggcta tgcaggtgcc gccgatcacg cttctcggca
     3001 gtgataaggt atatgatcac agctataccc tgtgcggaag tctttgtact accgccgatc
     3061 tgctcgttcg taaggcggaa ttgcccgagc ttaaaatcgg cgatgttctg gcgttcggca
     3121 ggtgcggcgc atacacggta accgagggaa cggttctgtt cctttcgcgc acaatgccga
     3181 gggttgtgct gtgttcggaa aaagacggct taaaactcgt ccgtgatttt tacgatacgg
     3241 acaaactgaa tttttaacaa aaacgaggaa ataaaatggg aattatttct ataaaatttt
     3301 ttgtgttcgc tgcggcggca attttgatat attatctttt tcccgtaaaa aaatatcaat
     3361 ggacagtcct gcttgccgca agctatactt attatattct caactgtaac aagtatgttc
     3421 tttatatgct tgtaacaaca gttactactt atttcggcgc aaggattctt gaatatgccg
     3481 ctgaaaaaag caaacttttt ttgcttcgga ataaagagag ctttacaaaa gaaagcaaga
     3541 aggaattcaa agcaaaaaca cagagaaaaa agcgcttgat tcttgccacc gttctgattt
     3601 ttaactttgg catacttgct ttcttaaaat atttcggctt ttgcgccgat tcggttcact
     3661 c
//
LOCUS       DAWBSW010000196         3604 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4293_length_3604_cov_2.139476, whole genome shotgun sequence.
ACCESSION   DAWBSW010000196 DAWBSW010000000
VERSION     DAWBSW010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_3604_cov_2.139476"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1805..1836
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1072 a          665 c          837 g          998 t
ORIGIN      
        1 ggtttttctt ttgaatgata cagcgcgaat tcgttcggtt gaactcggcg aatatatcgt
       61 taaaaacaaa tcgactgtca gagctgccgc aaaggtgttc ggggtttcaa aatcaacggt
      121 acatactgat gtaacgcaaa aattggagga gattgatccc ggtttatgcc atgaggtcag
      181 ggaaatactt gatattaaca aggcacagcg acatatcaga ggcggaattg ccacaaggga
      241 aaaatacaag ggcagcaaaa tgaggtgatt ttatgtgcgg aatttgcgga caggtttctt
      301 ttacaacgga tttatcaaaa tatgaaagtc aattttataa tatgcaaaat aagctttatt
      361 ccagaggtcc cgaccgacac ggtgaatttt tttctcagaa cgccgtattg atgcacagac
      421 gtctggctgt tgttgatata gaaaaaggca atcaaccgat gacgtatgcg gttgacggag
      481 ctgttttcac tctctgttac aacggagagc tttacaatac aaacgagata agaaatacgc
      541 tcgaaagcag gggatataac tttttcagcc attccgatac ggaggttgtt ttgaaggcat
      601 atgttgaata tcgtgaaaaa tgtgttgata tgttcaacgg tatttttgca tttgctgttt
      661 gggagcatga aacgagacgt ctttttgtcg caagggacag aatgggcgtt aagcccttgt
      721 tttattcgat gactaagagc gggttcgttt ttgcatccga aataaaggcc ttgcttgaac
      781 actctgaaat agagcctgtt atcgacacgc gttcgcttgc cgaaatcatg attatcggcc
      841 cgggcagaac gccgggatgc ggtgttttta agggcataag tgagataaga cctgcacatt
      901 atgcatatta tgatgaaaca ggcttaacaa ttcatcgcta ctggaatgtt gaggcgcacg
      961 agcactcgga cagctttgaa cagacgactg aaaaggttcg ttatcttgta accgattcaa
     1021 taaagcgtca gcttgtgtca gacgttcctc tttgcacgtt cctttcgggc ggactcgatt
     1081 cgagtctgat atcgtctgtt acaaacgggg aatttaagaa aagcggagag gtgcttcata
     1141 ctttttccgt tgactataaa gacaacgaaa aatatttcaa aaaatcaaaa tttcagccga
     1201 attcagatcc ggaatttata cggattatgg agaaatattt aggaggagtt catcaccgaa
     1261 ttgtgattga caccgatgag cttgtcaatg cactgtattg tgcggttgat gcacgtgatc
     1321 tgccgggaat ggcggatgtt gacgcttcaa tgcttctttt atgtaaagcg ataaaggaac
     1381 agggaacggt tgcgctttcg ggtgaatgtg ccgatgaaat tttcggaggc tatccgtggt
     1441 acagggataa aaccatacgt tcctccgagg gcttcccatg ggcgcagtca acggaatatc
     1501 gaatgtcatt tttgaatgat gaatttgccg aaaaaattga cggatcggaa tatgtctatg
     1561 aacggtacaa actgacggca gacagtgctc cgctttataa taaaatgaac gttgatgaca
     1621 tgaaaacggc gcagatgatg aagctgaatc ttgactggtt tatgcaaact ttgcttgacc
     1681 gcaaggatag aatgagtatg tattcgtcac ttgaggtcag ggttccgttc tgtgaccaca
     1741 gaattgtgga atatctttat aatgttccat gggaaatgaa ggatcacaac ggctatgaaa
     1801 aaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatc ctcggcgcca ctgtagggga
     1861 gctggccgag tagttgattg agtggtttgt tgaaaattta ataaaatatt attaacccac
     1921 ttcgtcactc cttcgtcgtg ccaccgcccc tgcaagggag gcaagaataa tttcagcgcc
     1981 gaaaagtgga taaatctcaa aataaatcgt tataacaccc tataaaaatt aaattgtata
     2041 ttgacccgac gttaaaaatc ctgtattata tttaaaaaag ggggatatac atgaattttg
     2101 tttataaaat actttctttt ctaaatataa ttacgctttg ccttgctatg ctttgtggaa
     2161 attatccggg cttcacggct gcggagcttg attacgaaac cgcatacggc aacccgatga
     2221 agggctttat gcctttctac agcgaggacg gtgccgatcc ctccgtgcca tacagcatgg
     2281 aatggctcta tattccgatg acggagcttg ttgcggacga cggcacattt acgcttcgcg
     2341 gaacgcttga agaaaagctt gataaaatcg ctcgccgagg tcatcaggcg gcactcagag
     2401 tttatcttga ctatcccgat aaggaatgtg ctgttccgca gttcatatgg gatatgggag
     2461 taaaaaaata tgaatacagc gagtacggcg ggggagtaag ccccgattac agtgaccaaa
     2521 ggctgattga ttttctgctt gattttattg ataaatttgc cttgaattat gacggtgaca
     2581 agagaatcgc atatattacg acaggcttaa tcggtcattg gggcgaatgg cacgtttgcc
     2641 atgaaatcaa gtccgcaatg gcttcaaaca agcagaaaag acagattatc catgcatttg
     2701 aatataagtt taagcatacg aatattctta cacgttaccc gggaacgccg ggttcgaaca
     2761 acggtcaaat cggcttccat gatgattctt ttacatacaa cactataaat tccgaaaaag
     2821 attatttctt tatcaatcag ttaaaaaaag cgttgaaaac aaatatttgg aaaactcagc
     2881 caatcggcgg tgaatttcgt cccgaaggtc aggataattt ccttaatggc aaggtgacgg
     2941 aagattttca ggactatgac gagtgcgtaa ataagacgca ttgctcatgg ctgatgatga
     3001 caggggcttt tacaggcaag ctgagtgacc gggagatcaa gacggcaaat gaagcctcgg
     3061 caaagctcgg atatgatttt tatgtcgata aagttcaggt caaaaagctt ttcggcaagg
     3121 cttatgtcag agtcggggtt aaaaatctcg gcgttgctcc gatttacgct aaccctgccg
     3181 tttatatcgg aacagaagat aacgaggttg agacaaagca ggacataagc aatcttttac
     3241 cgggtaaaac ggaatatttc accgcaataa tcgaccttaa atcgggtgac agcgttcata
     3301 taagaattga cgatgtttca aaatacggtg catatgtccg cttctccaac gtcggcgggt
     3361 caacctcatc cgacggcaaa tttataatcg gaacggttga gtaaaaataa cagtctgcaa
     3421 catttttaaa aatgcggcag actgcttttt tattgtatgt atttttcgta tggttcaaaa
     3481 ttttcttccg caattgtaag caaaaacgga attgattttt ccttaataag aagcggtttg
     3541 aattcatcaa gcttttgttt aatactttct tcatcaataa tcaacaagtt tgataccact
     3601 aatg
//
LOCUS       DAWBSW010000197         3604 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4291_length_3604_cov_2.372781, whole genome shotgun sequence.
ACCESSION   DAWBSW010000197 DAWBSW010000000
VERSION     DAWBSW010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_3604_cov_2.372781"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    305..314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    614..623
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3170..3244
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          857 a          911 c          717 g         1024 t
ORIGIN      
        1 gcacagacta tctcgccgcc gtgaattccc gcaccgggtc cgacatcgac aatgtagtcc
       61 gccgaaagca ttgtttcctc atcatgttca acaactatca gcgtgttgcc gaggtcacga
      121 agctccttga gtgtgttcag cagcttattg ttatccttct gatgcagtcc tatgctcggc
      181 tcatcgagga tataaagaac gcccataagt gacgatccta tctgtgtcgc aagcctgatg
      241 cgctgacttt caccgccgga aagtgttccg gagcttctcg tcagcgtaag ataatcaagg
      301 cctannnnnn nnnnatctct ctttgaaagc ttcaattcat caagaaatgc aagtgcctct
      361 ttgattgact tgctgcaaaa ttcgtaaata tttattccgc cgacagtgac aaaaagactt
      421 tcttttttca gtcttgcacc gccgcactcg gggcactcaa ccgctgtcat aagctgctct
      481 atctcgttgc gtgaatactc gcttgtcgtt tcggtatagc ggcgttcaat attgttgata
      541 atgccctcaa agtccttcat gtaggtgcct tcgccgtaaa cgctcctgcg ctgcatcttt
      601 atttttttgc cgtnnnnnnn nnncttcgga tcaggcacta caagcttttc gctaaccttc
      661 ataaaaattc ccagaccgct gcacttttgg caggcaccga acgggttgtt aaagctgaac
      721 attctcgggt taagctcctc aacgctgaca ccgtgctcag ggcaggcata gttctgagag
      781 aacataatct cctcgccgcc gataacgtca attataataa gcccatttga aaggcctgcc
      841 gccgtctcaa cggaatcgga aagacggctt cttatctcgt cctttataac gagtcggtca
      901 acaataacct caatattatg ctttttgttt ttctcaagct ttatctgctc cgaaaggtca
      961 tacgttattc cgtcaattct cactctcaca aagccgctct tgcgaacacc gtcaagctcc
     1021 ttgacatact caccttttct gccgctgacg atcggagaaa gtatttggat ttttgtgccc
     1081 gattcaagct ccatgattct gtcaacgatt gtatccacgc tctgacggga aatctctctt
     1141 ccgcagacgg gacagtgagg tataccgacc ctcgcataga gcagtctcag atagtcatat
     1201 atttccgtaa ccgttccgac cgtggagcgc ggattttttg atgttgtttt ctggtcaatc
     1261 gagatcgcgg gcgaaagtcc ctcaatgtag tcaaccttag gtttctccat ctgtccgagg
     1321 aactgcctcg catatgacga gagcgattca acatagcggc gctgaccctc ggcataaatc
     1381 gtgtcaaagg caagcgaaga tttgcccgag cccgaaagcc ctgtaaaaac cacaagcttg
     1441 tccctcggaa tttcaacgtc aacatttttc aggttgtgct cgcacgcacc ctttactatc
     1501 aatttattct gtcgcattac ttttcctctc tcaacttgtt tatgcggtca cgcagatatg
     1561 ccgcatgttc aaattcaagt atcttcgccg ccgctttcat ctcggcggtg agctgtttaa
     1621 tcatctgttc cttctcggca cggctcatgc gcttatgaga tttttcgttc tttgacgacg
     1681 agatttcaag aatgtctctt acgtcctttt taatggtctt aggcacaatt ccgtgttctt
     1741 tattatattt ttcctgcttt tcacgtcggc gcatagtctc cttgattgcg cgttccatcg
     1801 agtttgttac aacatcgccg tacataataa ccttgccgtt tgcatttctt gcggctctgc
     1861 ctatcgtctg aatcagcgag gtttcgctcc tcaaaaatcc ttccttatcc gcgtcaagta
     1921 tcgcaacaag cgacacctcg gggatatcaa gaccctctct caaaaggttg attccgacaa
     1981 gcacatcaaa ctcgcccagt cgcagatcac gaataatctt cattcgctct atcgtatcga
     2041 catcatagtg catatatcgt accttgatgc cgagattttc aatataatcc gtcaaatcct
     2101 cagccatttt cttggtgagg gtcgtaacaa gcacacgctc gccattttct attctcatgt
     2161 taatttcaga tatgaggtcg tcaatttgac cgtcggtcgg gcgcacggaa atctcgggat
     2221 cgagcaagcc cgtaggtctg ataacctgct cggcaatctg cacgctcttt tgtctttcaa
     2281 actctcccgg agttgcgctt acaaaaatct tctgcccgat tttattatag aattcgtcaa
     2341 aggtcagcgg tctgttgtcg taagcagaag gaagccgaaa tccgaaattg ataaggctgt
     2401 cctttcttga tctgtcgccg ccgtacatgc ccctgacctg tggcagcgta acatgagatt
     2461 cgtcgacaaa aagaataaaa tcgtccggga aataatcaag caatgtgaac ggaggttcac
     2521 ccggttttct gccggacatt attgcagaat agttttcgat tcccttacaa aagcccgttt
     2581 cacgaagcat ttcaatatcg tattccgttc tctgcttaat acgctgagct tcaatcagct
     2641 taccctcgga ctgaaagacc ttgacctgct cctccatctc ggaatagatc tgagttatcg
     2701 ccttttccat cttctcgggc gaaacaacgt aatgcgaagc gggataaatc gcaacatgag
     2761 aaagcacgtt tttcacctga cccgtcaggg cgtttatctc acttatacgg tcgatttcat
     2821 ctccgaaaaa ctcaattcta aaagcatttt cattagaata aacagggaat atctcaacaa
     2881 cgtcaccctt gaccctgaat ttgttacgag tgaagctgat gtcgttccgc tcatattgaa
     2941 tttcaatcag tttttttata agatcatcac ggttcttctc catacccgtt ctcagcgaaa
     3001 taaccatgct tcggtaatca atcgggtcgc cgagggagta aatgcaagaa acgctggcaa
     3061 caatgataac gtccctgcgc tcggaaagcg ccgaggttgc agagtggcga agcttgtcta
     3121 tctcgtcatt tatcgccgag tccttttcaa tataggtatc cgtcgtcggn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnatatac gcctcgggct gataatagtc gtaataagaa acaaaatact caactgcatt
     3301 cttcggaaaa aactccttga attctgagca gagctgagcg gcaagggtct tgttatgagc
     3361 cagcacaaga gtcggtctgt ttaccttttc aataaccttc gccatggtga aggtcttacc
     3421 cgatcccgtt gcgccgagaa gcgtttgctc catatatcct ttgttaagtc cgtccgtcaa
     3481 ttcttcaatt gcctgcggtt gatcgcccat cggttcgaaa ggtgaaacaa gttcaaaatg
     3541 atccattgcc aagcctcctg cccttattat aatcgtgccg ttttattata acataacaaa
     3601 ttta
//
LOCUS       DAWBSW010000198         3557 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4361_length_3557_cov_2.210737, whole genome shotgun sequence.
ACCESSION   DAWBSW010000198 DAWBSW010000000
VERSION     DAWBSW010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_3557_cov_2.210737"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          999 a          757 c          781 g         1020 t
ORIGIN      
        1 tagcttatcg tacaatttca acaaaatatt acactttttc aagtataatt gaaatatgaa
       61 ttgatttttt ccttttttta ctctataatt atctaaaaca attgttcgga ggtctaaaat
      121 gaaggatatt aacacgggaa cataccccac gatgattacg ccgtaccatg acgacaacac
      181 agttgactat gaggcggtca agaatatcac aaactggtac atagacaacg gctgcacagg
      241 tatttttgcg gtttgtcagt caagtgaaat gatttatctt tcgcttgaag agaaggtcaa
      301 aatcgcaaag actgttgttg aaacggtcaa ggcgagcgga aaaatgtttc cgttgttgcc
      361 tcgggtcaca attccgacga tatgaacgct cagatcgagg agatgacggc aattgcgaac
      421 accggtgttg acgctattgt atgggtcagc aacagacttg atcttcataa cgacggtgac
      481 gacgtttgga taaagaatgc cgaatatctt ctttcaaagc ttcccgagga tatcgccata
      541 ggtatttatg aatgtcctta tccctacaag agacttctca ctccgaaaat tcttgactgg
      601 tgcatttcga ctggcagatt cgctttcata aaagatacct gctgtgatcc tgatatgctt
      661 gaagcaagac ttgaacagct caagggaaca agcattaagc tcttcaacgc aaactcacag
      721 acatttcttt ataccctcag acgcggcgct gccggatttt cgggaattat gggcaatttc
      781 caccccgatt tatatgcctg ggtttgcgac aattatgaaa aagagcccga aaaggccgag
      841 agagtgcagg gatttcttac tcttacagcc tttcttgagg ctttggctta tcctcagacg
      901 gcgaagtatc atatgaacaa ggtcggcgtt ccgatgagcc tttggtcacg ttcctgtgat
      961 caaaagaaat tcacaaaata tcagagaatg ataattgatg aattgattca aacagaaaat
     1021 cacgtaagag acataattaa ataaaaataa ccctcaaagc tcagaccatc aaatgagctt
     1081 tgaggatttt attatattct gcttaaataa ttaagccatt gaaggctgat attttctatt
     1141 gaatgtgtct tgagaagctt cctgccgttt tccgaaagct tctttgcaaa atctggattt
     1201 tcgataattt caagcattgc ttcggcgagc tgttcgctac tgccgacatc cgtgaggatt
     1261 ccgctgactc tgttctcaat caattcatcg gggcccattt cgcagcgagt cgaaacgcag
     1321 ggaacgccca tggcaagcgc ttcaagaagt gcgttgggca ttccttcaaa atctgacgaa
     1381 aggacaaaaa ccgaggcgaa gtttacaaat ctgaccgctt gcggatcggt tccggcaaga
     1441 ataatcctgt tctttaatcc gagatattcg atctgatttt caagatcctc tttttcctca
     1501 ccgtcgccga aaatgaacag catatagtcg gggatttgat tggcaatttt tgcaaaagcg
     1561 tcaatcagca aatcaaatcg cttaagcttt atcagacgtc ctgtcgcgca gattattctt
     1621 ttgcgctcgg caggagggga gagggaataa acggtttcgt tgaatatcgc atttggtatg
     1681 attatatcgc gctcgctttt cttattgccg aaaaactgag ccgctttgcg tgtttgaaaa
     1741 acatagccgt ccgtgaaatt ataaaagaat tttctcagaa aggtgttaag cttagttgcc
     1801 ttatacctgt atggattgct tcgctccgtg ccaacagatt ttgttttggt aaacattgtt
     1861 gcaagaaccg cgtaccacgt catggcaaag ctcatgccga taagaacatc aagctgtaat
     1921 gatttaacaa gccttcgtat tgcaattatt ctttttatcg agccgaaaag tcttttcgca
     1981 gaagctgact gctccagctc tccgagctcg gctgtgcgga cggaaacgga ctcttcaagc
     2041 ggataaaagc tgtcaacgct gttgaaagtt ataatctcaa cgtcctgcga atgaagcgca
     2101 aagtaattag caagcgaaac cgttgctctt tcggctccgc cagaggatag attttttatc
     2161 aaaaatccga ttttcattta atcaccaagc tttttgaata cttttgattt ttcaagtccg
     2221 atataaagca ggaatccgag tgtgtagcac acaacaatct cgccgagagc gactcccgaa
     2281 gccgaggtga aaaatccgac gattcctgcc tgaccgggtg tgaacatcat tatttccgct
     2341 ccgattatta ttccgttaaa aactgtcggg aaaataaacg acagcagggg aatgttcttg
     2401 attttaatgt ttcttgtgag ccttgtcgcc agagcggcaa gaactgttgc gaccgttccg
     2461 aggagaatgt cgatcggtcc gtacgggctg ccgatattgg caatgatgca gccgattgca
     2521 agaccgggga tcgccgcagg ggtgaacacc gggaggacag taagcgcctc ggaaattctg
     2581 aattgtgttg caccgaagga aagcggggct acggcgtaag tcaacgccgc ataaattgcc
     2641 gcaatcagcg cagcctgtac ggtgtatgcc gtaccttttt tcatcttctt cattttttta
     2701 gctccttagt tttattttaa gtcaggatgg tttcgaactg actttttacc tttagtaact
     2761 cttgactaaa tcatagcaca attctcagcg gataaattta ctgaaatatt tcgcagagat
     2821 cctttatcga atcaacacag ccatctgctt tgatatttga tttttcgtag attttcggtg
     2881 tcgtaacgcc ggtcagaaca caggctgttt tcatgccgtg acgaactcct agagctatat
     2941 ccgcagcaag tgaatcgccg atgaagcata tttcatcacg tctgcaatgc aaaagctccg
     3001 tcagataatc cgcagtgtag acctccggct tgcccataac aagtggctca acgcccgtgc
     3061 aggttgagaa cagagccatc atagaaccgg cgtcgggcat atagtcgtcg cccatcggcg
     3121 ctttgatatc gggatgagtg acgataaatt ttgcaccttt tataataaag ttgcagcctt
     3181 tgcggatttt ctcgtaattg agagtatcgt caagaccgag aacgaccata tcgggcatat
     3241 tctcggtgag aggaattccg gatttttcaa agtcgtagag gaggcggctg tttcctataa
     3301 gataaactga tttatcgggg taattctttt taatatagtt aattgcaacg tgagttgaaa
     3361 cgataatttt ttcctcggga acggggaaac ccattttgat aagcttttct cgacaaacaa
     3421 ctgaattatt cgacgaatta tttgtcagaa aatagaaatc ctttccgttt tcttttacaa
     3481 ggtcaaagaa tttttttgct ccgtcgatca gctcattgcc gagatataca gttccgtcga
     3541 gatcggcgac ataatat
//
LOCUS       DAWBSW010000199         3555 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4363_length_3555_cov_2.404286, whole genome shotgun sequence.
ACCESSION   DAWBSW010000199 DAWBSW010000000
VERSION     DAWBSW010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_3555_cov_2.404286"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1123..1132
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          945 a          837 c          785 g          978 t
ORIGIN      
        1 acatggttct tatcacgttc aaaaagtttg cgtgctctgt cgatatatgc ttctttccat
       61 gccggatcgt tcgcaagaag gctccaacgc tcaaagaaga agccgtcaat cgaggtcgag
      121 aatccgtgag cctcaaggtc ggattcgtca attaccatga agccgagctg atcgcacatc
      181 tccaaaaatc tcgggtcgtt cggataatgc gaggtacgga cggcattgac attgtgacgc
      241 ttgaagatat aaagatcctc gagaacgtgc tcaatcggcg ttgcatggcc gtaaagcggg
      301 tgagaatcat ggcggttaac acccttgatt ttaatcttct gtccgttgag ataaagaaca
      361 gactttgaga taacaagatc cttgataccg accttaacga gtacgacctc atcggaaacg
      421 tcaagaataa gatcatagag cagcggatcc tcatcgctcc aaagaatcgg attatcgaac
      481 ttgattttta tcttaccgtt gacgctttca cccgagtcaa tgctttcgcc gtggggcgaa
      541 acaaggcggt agctgacctc tctgtcaccg acaaggtcaa gatccacgct cataatacat
      601 ttcgtgtatt tctttttaag ctcggttgtg acgtaaaaat cacggattct gcctctgcgg
      661 cttcttttga gaatataaac atcacggaag attccgctca ttcgccacat atcctgatcc
      721 tcaagatatg agccgtcgca ccacttgagc acaagcattg caagtctgtt tgaacctgct
      781 tgaacaaaat cattgatatt gaactcgctc gtcatgtggc tgacctgact gtagccgaca
      841 aatttgccgt ttatccaaag ataaaagcag gaatcgacac cctcaaagtt gagaaaaagg
      901 tcacgtgaaa ggtcgttctc atcaatatca aagtcacgga tataaattcc gcacgggttg
      961 tcgtcgggga catacggcgg atcgcaggga tatgggtagt ccgaattggt gtagttcgga
     1021 gcgtcatagc ccttgttgag cgccatttgc cagttcatcg ggacctttat tttgtcataa
     1081 ttgccgttaa tttcataatc tgcggcgtaa gagccgtcct cannnnnnnn nnctccctgc
     1141 cccacttcgg gttggatgcg aggaagtggc gctcgaggac ttccgagtga ttcgggcaaa
     1201 gcgcgaaatc ccattcgccg ttaagaagct gaaaatattt tgactcctct ctcgactttg
     1261 tgagagcgtt ctcctcattg agatagggaa tgaaatatgc gtgaggttct tcacagccga
     1321 catgtaaggc tgtcggattc tcgtagtatt ttggtatatt cagcattggt tttcctccga
     1381 tcaaagctcg attttagcct ttcttgagtt aatcgcgcgg aagatcgatg tttcgaactt
     1441 cggctttctc gaatatgtgt agagtccgtt ctgctcctgc tcaacgtcgt aaagctgagt
     1501 atagcagaat ccgaacatcc tgtcattgtc aagcaatgcg tccgtgaggc ccttgtagcg
     1561 ctcaataaat tcatacttgt tcttcggcgc attgccatag ccccacgcgt tctgttcatt
     1621 ttcgttaaca gaccagcgga ttccgccgta ttcgctgatg aaggtcggtc cgccggtgta
     1681 tttctgacgg cgctcatggt tatcgtagag cttaccctcg gtcatgagca tatcgaaatg
     1741 ctccttgaat accttcggat cctgctcata gtcatgaagg tcaaaaatat cggtgataac
     1801 atggaagttg ccgcttgtat caatacacgg acgggtctta tcgagtgcct ttgtcgtctt
     1861 ataaatgaga gcaagagcat cgtcgaactg ctttcttccg tcatagtccc atgtttcatt
     1921 gagcggacac cagccgatga ttgccgggtg attaaaatcg cgctcaacct cctcaatcca
     1981 ctccggaatt acggaataaa caatgttcgg attggaatgg tcaagtcccc agtttgggta
     2041 ttcaccccac acaatgtagc ccatcttgtc gcaattataa aggaatctcg gctcaaaaat
     2101 cttctcgtga agtcttgcgc cgttgaagcc gacatcaaga gatatctgaa tatctttgat
     2161 catggcttcc tcgctcggag cggtgtaaat tccgtcggga tagaagccct ggtcgagaac
     2221 aagacgctgg aaaacggatt tgccgttgat aaggaacttc tgaccgtcga gctttacgtc
     2281 acgaaggccg aaatagctgt caaccttatc ctcgttaaag cgaagctcaa gatcataaag
     2341 tctgccctcg ccgacctccc agagataagc ctctttaagg tcaaggtgag cggttatcaa
     2401 acctccctca gtctcaacgc ttgccgcacc catgattctc tcctgataga gggcaagagc
     2461 ttcaaacttg ccggtgccga tgacctctgc ctgaatatca acagagcagt tttcaacgtt
     2521 tggaaaaacc ttgatgctct tgattctcga atcgggaaca aactcaagcc aaaccgtttg
     2581 ccatataccc gttgtccttg tgtaatcaca gccgtgagaa taatactcct cgctctgctt
     2641 tcctctcggc tgaaggggat ttcttgtatc gtcctcggcg taaaccgtca gaacgtttgt
     2701 gccgccgttg agatagtccg tgatatcgaa ttcaaacgag atgtagccgc ccctatgcgt
     2761 gccgacctcc ttgccgttca catacacggt agccttataa tcaaccgcac cgaagtgaag
     2821 aattgttctc agatcggcaa acttctcggg aacggtaaat tctctctgat accaaacggc
     2881 atccatgaag tccttgtact caactcccga aagcacgctc tcggggcaga aaggaacatt
     2941 tattgttctt tcaagctttg tctgcggctc gtaatatttc ttagcaagac cactcttgga
     3001 cttgtcgatt tcaaagctcc actttccgtt gaggttcagc cactctctgc gctgaaactg
     3061 cggattcgga tgctccggac ggggtatatt gatcatatga tattttcctc cataattata
     3121 ttcagtttat cataccatat taaaaaagat tatacaagta attatatata ttttttacaa
     3181 aaaccgaggt gagaaaaaca aaggcttcct cacagtcaaa tatattcgct tccggcttta
     3241 tggcaaaaaa acttgtaacg atgccatatc gcagtctgca atttgctgtg ataaagaact
     3301 attcatatgt gtcgtgacag cataggatca aggtcgaacg atgacaagaa atgaccttta
     3361 agcaatttaa caattctaaa aagcatttct tcataaaatt tttatttttc gtcgtatttc
     3421 tattgaaaaa aaacggacaa aatgatagta tagtataaaa tagcggggtg tatttatgga
     3481 agttaaaaaa atggaatctc taaaggagct ttacaaaatc ggcagaggtc cgtcaagctc
     3541 gcacactatg ggtcc
//
LOCUS       DAWBSW010000200         3543 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4378_length_3543_cov_2.311640, whole genome shotgun sequence.
ACCESSION   DAWBSW010000200 DAWBSW010000000
VERSION     DAWBSW010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4378_length_3543_cov_2.311640"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          848 a          886 c          761 g         1048 t
ORIGIN      
        1 tgcttcttta tattttttct ttatctcaaa agaaataaga ataatttatt aagaatttgt
       61 tgcaaaatgt tgacattgta agataatagt gatataatcc gattgttaaa aacagaatat
      121 ggttctgaga ataactcagg atgatggagg cgcggtgctt tacaagtaac tttcgcaggg
      181 aaggcaactc agaaagtgaa aagactgacc gccgaagcgt gggaaactgc cttgtgaacc
      241 atagctggga cgttgcagaa tatgtaacgg actgtcacaa cttgtatgtg gagtgctgtc
      301 atgtagtaat agtcgttatg acatggttgg tatgaatgta cggaaccatg tcgtttttta
      361 ttttaattca ggagggttta ttgtgaaaaa atccaagaaa atcattttgg cgatcgcctg
      421 cgttgttatt gtcgcagttc tcgctttcgc caagatctcg ggcggttcgc tttcaacagt
      481 aaagtgtacc gactgcaatg gcacaggaat tgttgagcag gttgaatgcg aaacctgcgg
      541 aggcgaaggt gaagtcagag cttcctattg ggctcttgtt cccccggtaa tcgctatcgc
      601 acttgctctt atcaccaagg aagtttacag ctcattgttt gtcggtatcc ttgccggttc
      661 aattctcggc tcggacttca aattcaccaa gacaatggat atccttgtca atgacggact
      721 tatcggcgcc gtttccggca ctgcgggtat cttcattttc ctcgttgttc tcggtgttgt
      781 cgttgccctt atcaacaagg caggcggttc cgccgctttc ggcagatggg cacagaagca
      841 catcaagaca agagcaggcg caatgctcgc tacctttgct ctcggtgttc tcatctttat
      901 cgatgactat ttcaactgcc ttacggttgg ttccgttatg cgtcccgtta ccgacggtca
      961 taacgtttcg agggctaagc tctcatacct catcgacgca acagcggctc cggtctgcat
     1021 gattgctccg atttcctcat gggcggctgc cgtttcttca tatgctgagg acggtcaggg
     1081 tatcgctctt ttcatcagag ctattccgtt caatttctat tcgcttctta ctttcgtatt
     1141 catcatcggc atcacgctca tgaaatttga ctacggttcg atgagaattc acgagctcaa
     1201 tgccaagact aagggtgatc tttacacctg cggcgacaag gctgaggttg aggaagaaaa
     1261 tccgtcatca aacggtaagg ttattgacct tgttctcccg atcgccgttc ttattgcagt
     1321 ttgcatattc gctctcgttt atgtcggcgg aatcttcgac ggaagcaact ttgtcgatgc
     1381 tttctccaac acggacgctt cgataggtct cccgtggggc agcattatcg ctcttgtatt
     1441 cacaatcgtt tacctttgcg caagaaagct tgtttcgttc aaggaagcta tggagtgtat
     1501 tcccaagggc ttcattgcca tggttcctgc gatcctcatt cttacctttg caacatcgct
     1561 taagaacatg acagcggttc tcggtgcaaa gccgttcgtt gccgacgtta tgacaagcgt
     1621 tgcaggtgat ttccagaact tccttccggc tatcatattc ctcgttgcat gctttatctc
     1681 cttcgcaaca ggtacatcat ggggcacatt cggtattctt atcccgatcg ttatcgcaat
     1741 cttcccgtcg ggcgcaattc agacaatcgg tatgtctgct tgccttgcag gttctgtttg
     1801 cggcgaccac tgctcaccga tttccgacac gactatcatg tcctcggcag gcggtcagtg
     1861 caaccacctc aaccacgttt cgactcagct cccgtatgcg atctccgttg cggctatctc
     1921 gttcgtaatg tacattgttt cgggcttcgt tcagaaggca tacatcgttc ttcctatcgg
     1981 cgtcgttctt actcttgcat tcctctttgt aatgaagatg gctatggtaa agaagtacgg
     2041 caaggaaaac gtataaggaa taatcaaaaa tcaagggctg tcgcagagat gcgacagcct
     2101 tttttgtgta ttctattccc tgaatatcag gaaatttttg atattaaaat cctgtaatgt
     2161 ctcgattacc ggtacggtat tcgctgctta tacagctttc tgcttcagcg ttaagcaaga
     2221 ccaacgccgt aattcttgtt atgctgcctt aaatataaac tccgcagtca agctgaccct
     2281 ccttgtcaaa tctatccttt ctgaagcatt cgtagaacct gtatccgccg gcaaagctgt
     2341 aacagtcaaa tccctcctga gccagtattc gggaagctat atagctgcga agtccggact
     2401 gacagacaat atataccggc ttttctatat caagctcgtt tattctgtct ctgagctcgt
     2461 caatcggaat cagcctgtca aagccctcaa tctttcctga tgaatgttcc tcctcggtcc
     2521 tgacatctat cctctggata tcttctctgt ccttaagctt tgcaatgtcc tcacagtaaa
     2581 acagcttaac ctttcccgtc aaaacgtttt cggaaatata tccgaccatg tttatcggat
     2641 ccttcgccga agaaaacggc ggcgcatatg caagatcaat atccttgaga gtaacgatat
     2701 caccgccgga ctgcataaca gccgcaagaa catctattcg cttatcaacc ccgtcatatc
     2761 cgataacctg tgccccgatt atcttccatg tgtttatgtt aaacagcact ttcatcgtca
     2821 taagatgtgc tccgggataa tatgccgcat gggacggagg cgaaagaatt accttttcat
     2881 aaggaatatc aagctctttt gcccattttt cattcaatcc cgtagacgcc gcggtcaggt
     2941 cgtatatttt gattattgat gttcccgatg cgcctttgta cctgctgtca tatcctgcaa
     3001 tgttatccgc cgctatacgt ccctgcttgt tcgccggacc cgcaagagga acggagattc
     3061 tttttcccgt aacaaaactt tttatttcaa cagcgtctcc gactgcgtat atatccggat
     3121 ccgaggtctg catatgtctg ttgaccttaa tcgcacccct gccgccaagc tcaagaccgg
     3181 cagcctttgc aagaccgctt tcgggagtta ttcctatcgc catcaatacc atatctgttt
     3241 caataacgtc accgttatca agattgacaa tgatgccttc ctcaacgctt tcaaaaccgg
     3301 aaaccgttct gttcaggcga agatcaaagc cgttctcctt taaatgagaa tgaacaaagc
     3361 ccgccatgtc acggtcaaac tgcggcaaca cctgatccgc agcgtcaaca agcgttatat
     3421 gaataccgag gctccaaaga ttttccgctg cttcaagtcc gataaagcct ccgccgatca
     3481 ccaccgccga catgatatcg ttatagtcca ccccgttttt tatatggagc gcatcctcta
     3541 tgg
//
LOCUS       DAWBSW010000201         3495 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4440_length_3495_cov_2.393895, whole genome shotgun sequence.
ACCESSION   DAWBSW010000201 DAWBSW010000000
VERSION     DAWBSW010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4440_length_3495_cov_2.393895"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1058 a          759 c          738 g          940 t
ORIGIN      
        1 taagaattaa ttaatttttc ggaaaaatca ttgtgttttc ttctctttta tcttataatt
       61 tacgcagaat aggagaggag taatccaaaa gatgataaat attatagcct gcgacgatga
      121 tgcggacatt gttcgcgctt taaaaatata tctttcctct gagggctata acgtttttga
      181 agcacataac ggcaaagacg ttttgcgttt acttgccgaa aatgatgttc agcttgtgct
      241 catggacgtt atgatgccgc agatggacgg cattgaaacc acagccgaga taagaaaaaa
      301 atataatgtt cccgtcattc tcataactgc caaaagtgag gactatgaca aaattctcgg
      361 tctcaacgtc ggcgccgacg attatatcac caagccgttt aatccggtcg aggttgttgc
      421 aaggtcaaaa tcacaacttc ggcggtatct tgaactcggc aaggctccgg ccggtcagtc
      481 gcttcgcaac ggcggaattt ttattgacga tatttccaaa aaggttgtcg ttgacggtga
      541 agaggtcaac ttcacaccga cggaatactc cattctcaag ctttttatgg aaaatcttga
      601 ctgcgtttta tcgcccaagg agatttatga aaaagtatgg catgacggcg ttttcggaag
      661 cgaaagcaca gttattgtac atatccgtca cataagagaa aagatagaaa ttgaccctgc
      721 cgaccctcat tatattgtcg ctgtctgggg tcacggctat aagatgcaag gaaggggtca
      781 ctgatgaaaa taaagctcaa aacccttgcg gacaaaataa cggcggtagc tctctacctt
      841 atttgtatga caatagttat ttccctgttt cttgaagtca gctatatggc cgattacggt
      901 ttctatgata aatttcagct caccgaaagc acaactaccc gactgatgaa ggaaaaggtt
      961 gacaaggaat actccaatat tgaggaatac gttcttttgt cctttaagaa tcaaattaaa
     1021 aagctgcaaa gcagcgattc tgattacgag ctctaccgtt cgctttgcga aaaatacaac
     1081 aaacgtgata acacaaatct cattatttcc gcaagaacca acaacggtat gtataagctc
     1141 aacaatttgc aaaatggaga actgaacgaa ggcgagctgc tctcggacat tgtcggtaac
     1201 agtcccgaca aaaaaattca gacatgcttt ggaacgagcc gcaccacaag tttttttgac
     1261 gagaattcgg ctactatcag acctgtcaca attcatattt acataagatc ggatctttcc
     1321 gccgacgata cattcaggct tacatcaatc cttattcgca cggcaaacaa tcttaaatac
     1381 ttcattatca ttgcgatgtt catcgcactt attacaggca tggcaactgt cgcagtgctg
     1441 atggttaacg ttaactacac cccgggacat atgagattta tcgacaaaat tccgtttgat
     1501 ctgttctcga ttttgaccgt tgctatgaac ggcttcgtca tctcgatgat cgtgcttatg
     1561 tccgctgaca aaacccgtga ggatgacata gttctttgga atgcctcatg cgcaattctt
     1621 gcctttgtcg ttttctgcat aatgcttatt tacttccttt cgctggcagt cagaataaag
     1681 gcgggccatg ttttcagaaa tacccttatt tataaaataa tcgaacgcat cagaaaaggc
     1741 aattcggacg gcgaaaaatc cggctatttc aaaatgccct ttgtcggaaa gctgataata
     1801 accgtcggct tcgttctggc tctcgacctt gggctgatta tcttcaatta tttccgcttc
     1861 aagtcaaaag gtcttgcata taatttcccc gtttttgcgt taattcaggt cacaatggct
     1921 ctcgttgtcg gaactctcct ctttttcatt gcaataaact tcaaccgttt aagagagagc
     1981 ggaaaccgac ttgcaaacgg cgactatggc gaagatcttg attcaagagt aatgttcggc
     2041 gatttcaagg caataaacga cagcttcatt accctcaaag atgacgttat aaacgctctt
     2101 gaggagaaaa acaagaacga aaaaatgcga agcgaactta tcgcaaacat ttctcacgac
     2161 attaaaacgc cgctgacctc gataattaac tataccgata tcattcgctc aggcaaatgc
     2221 agcgaagccg agctgaatga atactacgag ataatcaaca agcaatcaaa gcggcttaca
     2281 aatcttttgc agaatcttat tgacgtttcg cagataacaa ccggctcggt caagataact
     2341 cttgacagtc ttaatctcgg tattttcatt tcgcaggtgc tcgacggtgt cgattccaaa
     2401 ctcgaagaaa aaaaccttac tctggatgag cgtcttcctc gggaggacat ctacataacc
     2461 gccgacgggc aaaagctttg gcgcatattt gaaaatcttc tgagcaacgt ctcaaaatat
     2521 gcgatgccga acacaagagt atatctgaat gttaaagaga ttgaggacaa cagagtaaaa
     2581 atcgaactga aaaacatttc ggagaaaaaa atcgaagcaa cgccggagga gcttttggag
     2641 cgtttcaccc gtgacgatac cgccagaaat accgacggca acggcctcgg actttcaatt
     2701 atcaagagtc tgaccgagct tcagggagga tctttcgaaa ttgccgttga cggcgacctg
     2761 ttcaaggcta ttcttatctt tgaaaaagcg acaaacggcg ctgaataatt ttttaccggc
     2821 agatcattac gatttgccgg tgctttttat tgacagcctc ggattaaaag attattatat
     2881 atttaataaa acttttcgga ggtgagttat ggcaaagggc ttacctaatt cacataaaca
     2941 catcagaaac ggtcaatctt ctctccgctt cggcgaggac ggaaaattcc gtatacttca
     3001 tctgactgat attcacgagg tcgatcccga aatggatgac gacgaaaacc gatcgattcc
     3061 gataaacaaa tccgccgaaa cggttaacgt tatctgccgc tgtatggagc ttgcaaagcc
     3121 cgaccttgtt gttttcggcg gcgacaatat cagcggctat tggcaggaat ttacatacga
     3181 ctatatgcgt aaaacgataa agaaaatcgt tgcgccaatt gcggaaaaga atattccgct
     3241 tgcaatcgta ttcggcaatc acgatgccga agccgagccg acctgcccct gtcttgcaaa
     3301 agaaaatcaa atcagcgttt actgcgaata cgataatttc agaggaacga tgaatgacga
     3361 ggacgtttac ggctgcggca attacaatct gccgattctt tcctccgacg gcaagcgtgt
     3421 tgcatggaat gtttggtgca ttgattcgaa cgactatgtt cgtgacgaaa actacaacgt
     3481 gataaaggac gaggg
//
LOCUS       DAWBSW010000202         3485 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4455_length_3485_cov_2.582216, whole genome shotgun sequence.
ACCESSION   DAWBSW010000202 DAWBSW010000000
VERSION     DAWBSW010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4455_length_3485_cov_2.582216"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          942 a          701 c          874 g          968 t
ORIGIN      
        1 gaataagaat cggcgtttac ggcactgcag ttgtccgtga cggaaagata acgtctgttc
       61 gtaacgttga ggggcttaat atccgcattc ccgccgaata taaagggtgt gcgttgccga
      121 tatataaccg cctttttcgc ggcagaatgc cgaatacggt tatttgcggc gcgccgatgt
      181 cgggtaagac cactgttttg cgggatcttt gccgtttgat ttcagacgag gagcacaaaa
      241 aaatcaccgt gattgacgaa cgctgtgaaa tgagcggata tgacctcggg atcaacaccg
      301 atgttttaaa aaactatcca aaggctcagg gtatcggcat tgccgtgcgg gctctctcgc
      361 cggatgttgt tatctgcgat gaaataggct cggtgaaaga ggcagaggag ctttgcacgc
      421 tcgtcaactg cggcgttaag tttattgtga cgatgcattg ctcggagctt tatgagctta
      481 aacgcagacc tcaatttaat ctgctcagtg aagcgggagc tacggaggcg tgcgtctttc
      541 tcaacgaaaa ggattttacg gtcaaaaaaa tactgatgaa cagaagctga aagggaaaag
      601 caatgaaatt attatcactt attgcattgg ggcttgcctg tgctatggcc ggacggtata
      661 tagcatacag gctcagccga cgtgttcgca tactcgagaa aatctcgctg atgttcggca
      721 tagcggagaa tgaaattaaa tttttgaacc gtccgacatc ggagcttatt gaactgctcg
      781 aaaaaaaaga cgagcttgcg gagcttgatt ttctgccgaa aagcatttct tatataaacg
      841 agggtatgga cattacggat gcttgggcgc aggcaattca tgagtcgaaa agcgttgacg
      901 gagaggacac atgcgttctc tattcattcg gagagagctt gggcagaagt gatgccgacg
      961 ggcagattgc aagctgccgc tatcatgtaa agcttacaga cgaacggctt aacgaagcgc
     1021 gcgaaaaacg taaacgctat gcttcgcttg cctgcggact cggaatgtta tctggaatcg
     1081 gagtttttat aattttgttt taggagcgga atatggaact ggtaatcaaa attgcggcaa
     1141 tcgggattat tgtcgccgtg cttcagcaaa ttctcacccg ttcggggcgt gacgaatatg
     1201 caatgctcgt tgtgctcgca ggtttgcttg ccgtcgttgc gatgttgatt cccgaattga
     1261 aagaccttta ttcttcggcg acctccgtta tgggattttg atatggaaat cataaaattt
     1321 gctttaatcg gcatactttg cgctgttctt gttgttgtcg tcaggcagca aagatccgaa
     1381 cttgcaataa ttgtccagct tgcaggcata gttgtccttg tgattttatc gattgaatat
     1441 atgaaaaata ttttcaccga aatggacggg ctgttctcgg acgcggaatt tctgaatgac
     1501 ggttaccttg aattgcttgt aaaaattctc ggaattgccg ccgtgacaaa aatcggttcg
     1561 gatatatgca aggacagtgc aaattcggca ctggcgaccg tagttgagct tgtcggaaaa
     1621 accgttatcc ttgccatgtg cctttcgctg ataaaaacgc tcgccgagat ttcaaagggg
     1681 ctgcttgaat aaaatgaaga ttaaaaaaat atcgggaatt gctttaattt ttgttcttat
     1741 ttctttttta ctttcagttt cggcgtgtgc ggaatcctat gccgacgaag tgaaagagat
     1801 ctataactct cttgactccg aaacgcagga gcttttaaag gatatcggtg ccgattcggt
     1861 agattttgac tccatgttcg gcgtcacccc gcagaaaatc tgggcgctga cgaaaaagct
     1921 catcacggga aatatcgagg ctccgacacg ctcggctgtt cgtcttgtct ctgtgataat
     1981 tctaattgca atttttgaat gcttcgctcc cgaggacacg agaatcaaaa caattgtcgg
     2041 cattgtcgga accttactct gtgtgatttc ggttatttct ccgctttcga cggctgtaag
     2101 ctcggcagtt tcttcaatag ccgtcggcga aaagctgatg cttgccctta ttcctgtgct
     2161 cgccgccgta gttacggtga cgggtaatcc gacgcttgcc gcgagctttc agtcaattgc
     2221 atttgcggga gctcaggtca tttcggggat ttccaacggc tatatagttc ctatcgtcgg
     2281 tgcggttatg gcgcttgata taacaggctc aatcatgccg tcatttaagc tcggcggaat
     2341 aacctcgttg ataaaaaaga ccgtaacggc tgtgctgacc tttgtttcaa caatttttgt
     2401 gtcattttta ggtatcaaag gtgcgcttgc caatgccgcg gacacggttg cgtcaaaggg
     2461 aataaagctc gtcataagct cggctgttcc tgttgtcggc ggcgcattga gcgaagcata
     2521 ttcgggaata atcggcagtc ttgtgctcgt caaaagcacg atcggagttt ttggaatagc
     2581 ctctgttgcg ctgataaatc tgccgtcatg cttacagctt cttttttgga tttttgcgtt
     2641 gaagctttcg gcggcagtcg gggaactttt taaccagcaa agcattgccg agcttctgcg
     2701 tgcgctggcc tcatcgctta cgcttttgaa tgttgtgctg ctttttaatg ctgttctttt
     2761 tattatttca acggcactta ttctggttat aaaggcggga taaaaatgaa tgagataaag
     2821 caatggacat tgggcatcac cgtttcggcg atagtcggtg cgataattct ttttatttcg
     2881 cccgagggtt caacggaaaa gctcgttcgt gcggctgtgt cgcttttcct catgtgcgca
     2941 attctcagcc cgttcattac aaatgcagac ccgatggaaa tttttaacag tattgaattg
     3001 acggagactc agtccgctgc gcaggcaggc aatgaagcag ccgaatatct gcaaaacgaa
     3061 ttaaaagaaa aaattcttga cattcttaaa gaatgcggaa taaataacgc agacataagc
     3121 ataagtatca atatagaaaa cggcaatgaa atgaagattg aaaaggtaga aatttttgca
     3181 gaaaaaaatg acgaaaatca atttgaaatt gccgaaaaaa atttaaaaaa ccaactggga
     3241 attgaaacaa aaatcgaggt gaaaaaatga aaaaagccgt tgaaaaaatt ctcgaaaaaa
     3301 ttaaacgcga caaaagaatt gcggcaatag tttgcgtcgg acttgcagga ataatattgc
     3361 tgactttatc ggagcttatg ccgcagggga aagatgtaac cgagaaaagc gataaggagc
     3421 cggtctccga tattcgagac agttacgagg atgatattga gaagagactg acatctattg
     3481 tctcg
//
LOCUS       DAWBSW010000203         3484 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4457_length_3484_cov_2.407991, whole genome shotgun sequence.
ACCESSION   DAWBSW010000203 DAWBSW010000000
VERSION     DAWBSW010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4457_length_3484_cov_2.407991"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2676..2685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a          802 c          784 g         1014 t
ORIGIN      
        1 aatcgttgtt atcagctcgg gtcagatttc cgatatcgtt gacgcaagaa cagctaccaa
       61 ggaacagctt ggtatgctta tgactaagac aaagggaggt aatgacaatg agtaataata
      121 ccgcaaagct tcatgagcct ttgtttcata ttgttaagcg ctcggctata ccgaaatggc
      181 agagctggct tgtaagaatc ggttcggtca ttgccgctct tatcgtttgt ggagtcgtta
      241 ccgttcttct tacaggtgaa gatccgattc aggtttatgt aaaaatggtc gagggtgcgg
      301 tcggctccga aagacgactt tggatgctcg cacagaatac ggcaatgctt ctttgcgttg
      361 cgctcgccgt tacaccggct ttcaagatga aattctggaa tatcggcgca gagggtcagg
      421 tgctcgcagg cggacttgca accgctgcct gtatgatttt cctcggcgac aagctttcac
      481 ccgttgttct tatgatagtc atggttgcgg tcagcattgt tgccggcgca gtgtggggac
      541 tcattcccgc attcttcaag gctctttgga atactaacga aacacttttt accctcatga
      601 tgaactatat cgcaatgtgt attgtttcat atttttcata tgagtggtcg gtgcccaagg
      661 gttcgggcca tatcggtgtt atcaacgaca cgacagaggc cggctggctt ccggctatcg
      721 gcggcaaggc ttatctttgc aacattctga ttgttgttgc aatcaccgta ttcatgtata
      781 tttatctcag atacagcaag cacggctatg aaatatccgt tgtcggcgag agcgaaaaca
      841 ccgccaagta catcggaatc aacgttaaaa aggtcattat cagaacgatg atcctctcgg
      901 gtgcgctctg cggcatcgca ggatttctgc ttgtcagcgg taccgatcac accgtcaacc
      961 gtgacacggc aggcggcagg ggatttacgg cgatcatggt ttcctggctt gctcactttg
     1021 atccgcttta tatgattctc acatctcttc ttcttgtttt ccttgagaga ggtgcaggcg
     1081 agatttcaac gaccttcgga cttaacgaat cgttctcgga aatcatcacg ggaatcatca
     1141 ttttcttcat catcggcagt gaatttttct taaactatca gttgaaattc cgtgagaagg
     1201 aggcgtccaa ataatgttag ttgcatttat tcagcgtgcg gtcgttcagg gcattccgct
     1261 tctgttcggc tcaacgggag agatcctcac cgagaagagc ggtaacctca acctcggtac
     1321 ggcaggcgtt atgtacgtcg gcggcatcac gggagttatc ggcgcattcc tctatgagca
     1381 gaatgcgact cagatgaaca ccttcctcac cgttctcatt ccgattcttt cgtcgatact
     1441 cggttcgctt gtaatgggac ttatctattc gttccttacc gttacgctcc gtgcaaacca
     1501 aaacgttacg ggtcttgcac tcacaacttt cggtgtcggc ttcggcaact tcttcggcgg
     1561 ctcgcttatc aagctcacga aaagtgacgt tccgtccgtt acacttacgg caacgagcct
     1621 tgcgttcaag aaggcgcttc cgtttgcttc aaagctcggc tggttcgggg agattttcct
     1681 ctcatacagc ttccttgcct atgttgcaat cgcaatagca attatagttt cgttttattt
     1741 taaccgcaca cgttcgggac ttaatctccg tgcagtcggt gagaatccgg caacggcaga
     1801 tgccgccgga atcaacgtcg gcaaatacaa gtacatttcg atctgtctcg ccagcatcat
     1861 agcaggcttc ggcggtctct actacgttat ggactatgcc tgcggtgtat ggtcaaataa
     1921 tgccttcggt gacagaggct ggcttgcaat tgcgcttgtt atcttcgcta tttggagacc
     1981 gaacctcagc ataatcggtt ctattctttt cggcggactc tttattgtgc acaactatat
     2041 tccgaatctg agcatgagcg atcaggagct tttcaagatg actccgtacc ttgttacgat
     2101 cattgttctt attgttatca gcatgctcaa gaagagagaa ttccagccgc cgcagagtct
     2161 gggactctct tacttccgcg aggaccgcta agcacttaat ccgaattgtt tattttggcg
     2221 aacaaagccc ttttcccgac ttgacgtata aaacatacgc ctgcgtcgtg agaaagggct
     2281 ttcttcatcc aaactaagca attctttttt attaagaata attgacaaac aaattttttc
     2341 tcaccttgtc gtacaggagt acgcctgcgc tgagataaaa tgtcttcttc atccgaacta
     2401 aactgcccga tttctattta tttataagaa aaaaggacga ccgacggccg ccccgacttt
     2461 cagcatctat tcctttttca atttgtactg ttcaaggtca agcaggataa ccgtgccgct
     2521 gtcaatctcc gaacgtgcgg tgatgccgat accgaggtta tcgcaaattc gcttgcaaag
     2581 ataaagcccg agtccgcttg cttttgaaat gattctgccg tttccgccgg tatagccttt
     2641 ttcaaagatc ctcggtaaat cttcgggtgc gatgcnnnnn nnnnntgtcc tcaaggcaaa
     2701 ggtactgctt gtctatgtaa atcttaatgc agccctcacg ggtgtattta agcgcattgg
     2761 aaagcaactg ttcaattaca aacgcaagcc atttttcgtc tgttattatt tttttgtcaa
     2821 gcggtgaata ttcaagcttg atttttcttt cgatgaattc agtcgaaaag cgtttgatcg
     2881 aatctctcac gatctcatcc aaatcatgct ctttaaaaac gtaatccgtt gaggttgaat
     2941 cgactcgaag aaaagcaaga accatctcaa cgtattgctc aatgcgaaaa agctcggaag
     3001 aaagtcggcg tgaaagggga gtgtcctcac tctgaagcgt gatgtgcatt gacgcgatcg
     3061 gcgtcttgat ttgatgtgcc caaacggtat aatagtcgat catgtcatgg tatttcatgg
     3121 agcttttgta ttcgatatcc gagatctcgt ctgtcagatt tttgataagc ttttgataat
     3181 cggcttcgtc aatgctctta attttaggga gattatctat catcgaggag gtgagatgcc
     3241 tgatttcgca aagcttctca tgtttctttt tgacggaaag aaaatcaaaa acgaaaaaca
     3301 caacgtctat tgccaaacaa agcagaaaag ggtataagac cgctttaaat gtaatgttgt
     3361 aaagtacaaa caccacagaa aaaacaattg aaaaaatcag aaataaaacg attccgctgc
     3421 gccgttgttt cagatattca aaaaacagtt tcattttcag cacccttatt caataatata
     3481 gccc
//
LOCUS       DAWBSW010000204         3448 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4511_length_3448_cov_1.860595, whole genome shotgun sequence.
ACCESSION   DAWBSW010000204 DAWBSW010000000
VERSION     DAWBSW010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4511_length_3448_cov_1.860595"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    324..333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    763..772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1117 a          660 c          633 g         1018 t
ORIGIN      
        1 cttctttatt gtggttatat tataccatat tgatatcaat aatacaatta acataatcaa
       61 caaacatttg atatcaatat gacttatctt tggttaaaat acatattgat atcaatttac
      121 aatgtgtgct ataatagtta tgctgggata ggtattctct ttcaaaactc atacaaagta
      181 ggtgagacac aatgtcaagt gatattacca aaaaaagaaa agccgtttat aacgacaaag
      241 taaataaaac gacacaaaaa tatgtcaaag agcattacga tagcttcata gttcgtatgc
      301 caaagggcaa gaaagaacaa atannnnnnn nnnaataaag acctttgccg aaagtaaagg
      361 tgaaagcctg aacggctttg taaaccgtgc tattgatgaa gcaatggacg aataagcggc
      421 aagctcggcg atttaagccg ttttacaagt caagtagcag gaaatacacc ataaacaaaa
      481 taaccgcccc accaggcggc agatttacgg gtacgccgaa aacaaaagga tcgcaacgca
      541 gcttgaagct gcccgaaaac gttattgatg tgctgcgtga atataaagcg cagcagaaca
      601 aacaaaaggc ataaaaagac ggtacacagt agcaagtgta ccgttatatt tttgctattc
      661 gttgagcttg caagctcgga tattttaata aaaaaagaac tccaaaaaat aaatttttga
      721 agcaataaac accaaataaa caccaaaacc gaaaaagtga gannnnnnnn nntgagaaaa
      781 agaaaaagcc gcaaaccctt gataaataag gatttccgac ttttataata tggtccgagt
      841 ggcgagactc gaactcacgg cctcttgaac cccattcaag cgcgctacca cctgcgctac
      901 acccggatac ctgagttatt ctaccacaaa ttttaaaaaa gtcaagcatt tgtttaaata
      961 atcttttaaa atcttaaaaa aacatttgtc gcacggttaa tatatacagt tttcataata
     1021 agcttcccac tgcttgatgc aaaaaatcaa cctaaatcaa cctaaatata atgaattaaa
     1081 aatctttttt tcgttttgct aacattagct atacaagatt taatatttgt caggttaata
     1141 attttatctg cacgaatgtc acaaaataaa atacagaaat atatgcgtta tgacaataac
     1201 atcaaaaaca taatttttgt ttttgttatt tcttactaat tcgctatcac attttacaaa
     1261 agtattgctt ttataagaac attgtgttat taaagtttaa ataaaaaaca atggattaat
     1321 atgtttattt ccattccaga aattcagttg gattaaaatg attaaaaagc acaaccctat
     1381 tacattttaa ataacggagg taaggcataa aattatgaat gattccattt ttcaacacaa
     1441 aaacggaacc ggaaaagaaa tatcggcatg gagcatattc agtctgatat taaaaaaatt
     1501 tcattggctc cttctcagcg gtgccgcgat tgcattggct gtattttttg ccgtttcttt
     1561 tcttgttaca ccgacatata aatcaaccgc aactttctat gtatataaca gttcaaacgg
     1621 ggtatcaacc tcgggagtaa tcaataataa cgaccttcag gctgccgaga gccttgcttc
     1681 tacatattca aaaatattcc agagtaatgc ggttctcgat tcggttttgc aggatttgaa
     1741 ctacaagaac aagctatcaa gaaaagagct ttccgaaatg gtcgatgttt ccattatatc
     1801 cgacactcgg cttcttgagc tttcggtcac aagtcccgat ccttaatttt catgcaaggt
     1861 tgctaaatca ttcatctcaa ttgctccgct ggagattgtc agaatcacaa aagccggcgg
     1921 agtcgaggtt gttgaccggc ctgaggttgc gaccgaaaaa tcctcaccga ggactgtttt
     1981 cgatacagcg atcggtttta ttgtcggaat aatcattgca tttattatta ttgttatccg
     2041 agcggtatcg gataccactg tttacctttc ggaggatctt gaaagtatac ccggcttgac
     2101 ggttctcggt cagatcccga acataaatgt ttcgggcaga tacagttatt ggtcattgaa
     2161 ggaaggaggc gtaataagtt atgaaaaaaa tatctttcat ggatatccga cggctatgtt
     2221 aaatatctcg gaagtgatat tcatatgctc ggcaacggtt acagggaatt tgaaaaaagc
     2281 aagaagttac tcggcaagca ttaaagccgt aagaaagaag gatgaaggaa ttggcataca
     2341 cacaagaagc taagcctttg gtctccgtca ttatgccctc atacaatgct gaaaaataca
     2401 taaaagaagc gatagaatcc gtaatcgctc agacatatac aaactgggag cttttcgtta
     2461 ttgacgacgg ctcaaccgac ggaagcggaa caattcttga cgggtacgcc cgttccgacg
     2521 atagaattca tgtttttcat caggagaaca gcggtcagtt tttcgcaaga gaaaaaggat
     2581 tctcaccgca ttattcttgc gattgtttcc gtttttcttt tatactgcgg actgctttcg
     2641 gcatcaaagt catttattat aattactgct tgtctgtttt tcgtttggat acctaccctg
     2701 cttaaaaagc aaaacagagg ttcaaccgcc gccaaagttt ttataggctt gctttgtgcc
     2761 ggaattatag tgctttcctc gacggcattt caggagcttt ttaaaatcat tgacacccgc
     2821 ttttcctacg cttcaagcgt ttcggagctg accacgggac gaaccgacat ttggcaaact
     2881 tacattaacg agtttataca caatccggtt cttctgctcc tcggcgaggg cttttcgagt
     2941 gttatcatcg gcaaaagagc ctctcacaat acgataattc agggcgtgtt tcaattcggc
     3001 ataatcggat ttccgttttt gattgcatgg tttgtgatta cgataaaaaa tttgatttca
     3061 aatatttcag ataaaaaacc tcatttttta tacatacttc tgatgggagt cggaatcgtg
     3121 cttccgtgga tggcattgga tattattttc ttcgatgaat tattcccgct gcccgtttac
     3181 ggcgctctcg ctgttgctta cagctcctta gagcttgaaa acagcaaata acatagaaaa
     3241 acaacggaag aatcaaactg ttcctccgtt gttttgtttt attgaatttg aaagcggcat
     3301 tatattatca ttttttattg atgcatcgag ccgcataagc gccgccgatc ggaatcgctc
     3361 actatgacat tgtcaatttt cgtcaggcac gaataataaa atataacgtt ttcaatttaa
     3421 ctgtttttgc tttgctccgt accttatc
//
LOCUS       DAWBSW010000205         3391 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4610_length_3391_cov_2.688849, whole genome shotgun sequence.
ACCESSION   DAWBSW010000205 DAWBSW010000000
VERSION     DAWBSW010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4610_length_3391_cov_2.688849"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1007 a          685 c          787 g          912 t
ORIGIN      
        1 aaaaaacaca tcagaaatca aaaacaaaag aaaaagagca ataattcttt tcataaagat
       61 atctccttgt aataataatt tgttttctgt aaaatatatt acaatgaaat aataaaattg
      121 tcaaaaaatt tttataaatc ctcttgacat ttaaaaataa tcggttataa taacagtaac
      181 gattactatt attgatgagg taaatctatg gcagctctaa aacattcaaa acagcgtgaa
      241 gcaattcttg cagagcttcg ttctcggaag gatcatccca cagccgagga tctgtacttt
      301 acgctcaagg ccgaaatgcc gaatttaagt ctcggcacag tgtacagaaa ccttaatatg
      361 ctcgcttccg acggcgttat tctcaaaatt agctttgagg gtgccgaccg ttacgacggc
      421 aataaagagc ttcattatca tttccgatgc tttgaatgcg gcagagttac cgatgtggat
      481 atgcccccga tcaatgatat taattcaaaa gcgcaagcat atgcaaaggg acggattgat
      541 actcacgaac tgattttcag cggtgtatgc aatgagtgtc tcgatgctaa ataaattttt
      601 tggaggtaaa taattatggc aaaatgggtt tgcagtgtat gcggttatgt tcacgagggc
      661 gacactcctc cggagaagtg cccggtatgt aatgctccgg catcaaagtt caacaagcag
      721 gacgaaggaa tgacttgggc agcagagcac gttgtaggcg ttgcacaggg tgtatcggat
      781 gatatcctcg aggatctcag agctaatttc aacggtgagt gctctgaggt aggcatgtat
      841 cttgcaatgg caagagtagc tcacagagag ggctatccgg agataggtct ttattgggaa
      901 aaggcagctt acgaagaggc tgagcacgct gcaaagttcg ctgagcttct cggagaagtt
      961 attacagact cgacaaagaa aaaccttgag atgagagttg cagccgagaa cggtgctaca
     1021 gccggcaagt ttgaccttgc caagagagct aaggaagcaa acctcgacgc tattcacgac
     1081 acggttcatg agatggcaag agacgaagcc cgtcacggca aggctttcgc aggtctcctc
     1141 aagagatatt tcggcgaata atatatcgta atataatttc tgtcctctga tttctttttt
     1201 gaaatcagag ggctttttat ttcaaaataa cggacgtacg atattgtaac ctattgtaaa
     1261 ttgtcttaac ttgacaaatt tagccttaaa tgttatcata aactaaaata atctgagttt
     1321 cggaagaagg tatatgatga tcaattcaga taatttatgt atgagctgca tgaaggatat
     1381 cggaacggaa aagcagtgcc cctactgtgg cttccatgcc gactcaaagc agattgagcc
     1441 ttacctgcct ataaggacgg ttctcggcaa cagatatctt gtcggaaagc ttcttgaata
     1501 taacggagac ggtgcaacct atatgggtct tgatctctca acgagagagc ctgttaatat
     1561 tcgcgaattt ttcccggaag gaatagcgtt gcgtgattct aaaacactcg gcgtatatgt
     1621 tgctccggga aacgaaaata tttttaatga atgtctgcaa agcttcattg agatgtggag
     1681 aaagctcatg cgtttgagcg ggctttcagc gcttataaag gtaaaggata cccttgaagg
     1741 caacggaacc tgctatgcta ttacggagaa tatcgacggc ataacgctca gagaatatct
     1801 tctccgaaac aatgtaggct atatttcctg ggaaaaggca cgtccgcttc ttatgcccgt
     1861 gctttcgacc ctcggaacac ttcacgcctc cggaattatc cacagaggta tttctccgtc
     1921 aacgcttatc gttgcttctg acggtaaaat tagaataaca gggttcagca tcagccaggt
     1981 cagatctgca agaagcgagc ttaaaagcca gctttacaac ggttatgcag cgtttgagca
     2041 gtacggcttt gacggaagac agggcacatg gacggatatt tacgggtttg gcgcagtgct
     2101 ttacagaact cttatcggaa ccgatccgat cgacgcaacc gaacgtgtaa caaatgacag
     2161 gcttatggtt ccgggcaagt ttgccgaaca gcttccggca tatgttatca acggacttgt
     2221 aaatgctctc cagattctcc cggaggacag aacgagaacg gttgaggagc ttcgtgcaga
     2281 gctttctgca tctccgagtg ccgctgcggg agccggtgtc gaatacgatc ctgttgtacc
     2341 ggtgaacgtc aaaaagcctg ttgcacagcg tattgtcgac gaggaggatg acgaagagga
     2401 ttacacttat aataagcgtg aggcagccaa gaagcgccac aacagatcct cggcaattat
     2461 tgcggtttgc gcggtaataa ttgtgcttgc aatcggattc cttgttcttt ggcagacagg
     2521 tgttattaag cttgataacg actctacaac gacagccgct tcggaaaccg ttgaagttcc
     2581 taattttgtc ggcaagccgt ataatgaaat catcgacaac acttacaaca cgaaaaactt
     2641 taagtttgaa aagagcgaag tctacgattc gacggttgaa gaaggctacg ttatcagtca
     2701 gagtgtcgat gtaaacgaca aagttcctgt cggtacaaca atcaagctcg ttgtcagcaa
     2761 gggcaagcaa agcattgttt tcccgacctc cggcatagtc ggaatgaaat atgatgacgc
     2821 taaaaatatg ctcacaaagc ttggatttgt tgttgagaaa tccgttaagc ttaatacaga
     2881 taacgagatt cccgatacgg ttcagggcgc atcgcttgaa gagggcgcat catatgaaaa
     2941 aggcacaacg gttttccttt ttgtttgggg tgaacccgag acaacagagc ccgttacaga
     3001 tgaaacaaca tcaggtgatg atttcactct tgatgacggt tcggtcagtg aaactattga
     3061 cagactcatc aatttcggca gaggaaatta aataaatcaa aatagaaaca gcagcggaaa
     3121 taaaaatttt gctgctgttt ttttatttaa caataatatc ggctgtttgt cctctcgttg
     3181 cctttaatat atcggcaggg gagatctcaa tctgacttcc tattcgtcct gcgcttatcg
     3241 ttaccgtttc aagtgaattt atatcctcgt gaaaggtggt tttaaagctc tttttcattc
     3301 cgataggact gcatccgccg tgcacatagc ctgtcagagg aagaagctcg ttttgcttaa
     3361 tcatttcaat tgatttttct ttgaccgctt t
//
LOCUS       DAWBSW010000206         3344 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4677_length_3344_cov_2.579203, whole genome shotgun sequence.
ACCESSION   DAWBSW010000206 DAWBSW010000000
VERSION     DAWBSW010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_3344_cov_2.579203"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          938 a          755 c          680 g          971 t
ORIGIN      
        1 gcagtaaatt taacaaaaaa ataaaacagt cgtagcaccg accaaagcac aaacgactgt
       61 ttaggttaga agtttccttc tgtaaatatt ataatgcaga atggaacttc tttcaacacg
      121 aattttgaaa ggagttttta ttatggcgaa attatcaaaa cgagcaaaac aagaatggga
      181 tttcttcata aatctcgaaa cgggcagacg aacatacaac aacctttgcc gaaaatgcaa
      241 aaacaaacgg ttgatatgaa aatgctgtat caaggactta tggaatttac tgaaatgaaa
      301 cagcttacac cgaccgttgt caacaaactt attgagcgaa tagaaattca taacaacgag
      361 aaaaagcatt cgcataacaa tgttaaagtg gacatttatt ttaccgctgt cggcccgttt
      421 gatattccga gcgaacaaaa gcttattgaa acaatgaaca aaattcaaga gcaaaacaaa
      481 tccgatgaaa aatcggcata aagaaaaagg tgtaacctca tttctgagat tacaccatac
      541 tttctaaaat acttccttat taaccctgag caaatccaca gcggtttttt aatgttaata
      601 taagagctta tttcttgaaa agaccgaaca gcccgcccct tttttcttct tcggctgttt
      661 caattgacat gaccttgtag ccggtttcat caatcacctt gcgaagctta tcttcatcga
      721 tcggcttttc cgttaagatg accgtttgcc tctttgtgtg ggaggaagta actttcttga
      781 ccttgaattc acggcgtata gcatcattta tatggctttc acacattccg cacatcatgc
      841 cgtcaatttt cattgtgatc ttcaacattc gtatcatcct ttctgtttgc tatgttatat
      901 agcagcgctt ttaccttggt tgtgcaagca aaagctatgc cgattattgc aacggagagc
      961 aaaatctctt tgacttcgct cattccgcac ctttaagagc ctcgaccatg tcgatttttt
     1021 tgttttttcg tgaaaccata agactgacca gaagcgacat gccgaccgtt agcgcaatgc
     1081 tgattgcgac agataatacg ctgacctgca ttttcatttc atattcaccg gcaagcgctt
     1141 tcagcagata atcgagcgca ccgattccgg caggtatacc gagaattacg ccgaaaacgg
     1201 agagccagag gttttgacct ataagaagct gaccgatttt tttgtcttta aatccgacaa
     1261 ccttgagagt tgccatctca cggtaacgct ccgtatagct cataacgccg aggttgtaaa
     1321 gaacaacgat gccgagaaca agcgcaccga gaacgagaat aatgatcatt gtgttaaata
     1381 tatcaatgaa agtttcaaac gaatccatta tcatttgctt tgactggata ctctttatac
     1441 aagtatctgc ggaaatatcg gatttctgcg tctttgtgta agcagaatca aaagcgaaag
     1501 gaagcttgat ttcctttgca taatcggagc ttatcgcaat gctttcagaa acacttctca
     1561 ttattttgct tatctttacg gtatattcat tgtccgtgcc aaacgggctg aacgtaagct
     1621 catcgccctc tttaacgtcg aattgatctg caagcctttc acagatcaat gcgccgtcgt
     1681 cgggaagctt tacatagtcc atattgtcgc cggggaaagc aacaagctca tgttcgattt
     1741 tataaacatc gaccgaaacg gttttctcct ccgcctgaac gctgacagat gcgctgaggt
     1801 cggcatcgta tttttcggcg agcctgtttt tctgctcgtt cgttgcattt tctgcaaaat
     1861 agattcttga cgaatacttc attgcgccgt catagtaggt atcaaggaaa gaattcattg
     1921 tatctctcat gccgaacgat gccataatga gcagtgtgca gccgacaatt ccgagaaggc
     1981 tcattgccga acgggattta tgacgaacaa tgtcacgcag attccaccgt gtgccgaatg
     2041 aaagcttatg gaaaagcttt gttctttcga taatcatcgg cttcattttc ttcggcgaat
     2101 acggtctgag cgcatccgcc gcagtgccgt tgagcatctt tctgaccgag agaaatccga
     2161 taagggtaag aacaacaatt attgccgcaa gaatgtagta gcagaagctc ggaatataga
     2221 gcttccattc gggcatatca aagtatgtac ccatcattcc gtcggggttc ataatatacc
     2281 atgcgataaa aaacccgaga cccactccga ttgccgaacc gataattcct atcataaacg
     2341 catatgaagt gtagtgcctg agaatgcgcc tgttcttgaa gccgagcgcc ttgagtgtgc
     2401 cgatttgcgt tttctctttt acggtcaggc ggtgcatcgt cgtaaccatt gtcagaacgg
     2461 caataagcag aaacaatgtc ggtaaaatcg aacccattgt ctgtccttct tcaacctcac
     2521 cgtgagcatt ggaatatgaa gcggactcgt ccttggcgag aatcatggca gtattgccga
     2581 gagcgtcatc cgctttttcc gtaaagtctt ttttagacat tgatgaaaca acgtttatct
     2641 gcggataata gtctctgccg attgtctttt cgtacataac aggtgagata taggcaaagc
     2701 cgaacgtgtt gtagtcgggc ataagctggc tcgaatcacg aacgcatata agctgttcgc
     2761 ctgctttgac aagacctttt ataacgcctt tcatctcaat gcctttgtaa gcaagagtta
     2821 tgctgtcacc gatcttgagg tcgtttgcct cggcatattt gtcggaaagc catatgccgt
     2881 ctgtgctttt tgcatcatat ttctcgccgt ccgttataag cactcccgaa accttttcat
     2941 tcgttgttac cgtgagcgca acggcgtcat cttttctctc cttaacgtcg gcggcaatgc
     3001 tgacatagcg tcctgcattg tcaactccgt caatatcgga tattttcttc aggtcggcgg
     3061 cggaatagcc tttttcggag attatcctga aatcggcata gcctgttttc tcaaagaagg
     3121 acgaggtgtt accctcaatg ctgtaccatt ccatattgaa gccgacaaag attcccgtgc
     3181 ctatagcaat cataataatc atcgagatga attgtgcacg gtaaagaccc atcgttctcc
     3241 atagtttttt gaataacata tcgtcacccc tttaccagtc gatactgtcg gcagaggcgg
     3301 gatttttttg ttcctcctgc gaaacgattt ttccgttttt aacc
//
LOCUS       DAWBSW010000207         3318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4718_length_3318_cov_2.284094, whole genome shotgun sequence.
ACCESSION   DAWBSW010000207 DAWBSW010000000
VERSION     DAWBSW010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4718_length_3318_cov_2.284094"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3012..3021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          757 a          807 c          788 g          956 t
ORIGIN      
        1 gattcatacc gcttttgctc aagaattgag gcttctcgga agcaaagtat tctgtcgaga
       61 attccgttgc ttcaaggtct gagggtgaaa cctttgccat aatgcctgca agcgatgaat
      121 acgggtattt atactcggtt ttgcgcttta ttttttccaa aagatcgggg tcggcggcaa
      181 gcttttctcc ctcaccggta ctttcaaccg gtgaaccgag ctcttttatc aattcaaagt
      241 gcatatttct tgctgccggg agggttatcg gcgagttttc accccaaagt gacgctgcgt
      301 ctgggtgtcg gataaaaccg agtagaagcc agtctgccat tgaacttttt gaaagcagag
      361 caaagggcat tatatgctcc tctgtgccga tttcacccct gattgcagaa agcttctttt
      421 ccggattgtc atatcttgtg atgcaaatca gtctttcctt ggctctcgtc attgcaacgt
      481 aaagaactct cagctcctcg gaacgtccgg aaattttttc ggcaatgtca attgcttttt
      541 tgccgacggt cggatatttg catttgcgtt ttgaatcacg ccgctcaaag cagataccca
      601 tatccgggtg gtaggcagca acattccttg cggcatcgcc gttgaacggc gcattgaggt
      661 cggcgattat gcacacgggg aattcaagtc ccttgctttt atgaatcgtc ataatatgca
      721 caacgtctgc ggcttccgaa acgtctgcag cggcttcaag gtcgctgttt ttacgctgaa
      781 ttctgtcgat gaaacgaata aagcccgaaa gtccgcgctt acccgagtct tcgtatttct
      841 ttgcgtagtc aacaagcatg ctgaggtttg ctttgcgctg tgagccgttc ggcatggcgg
      901 aagcaatggc tttacagcct gttttttcaa acatttcttc aaggaatcgg tcacagggga
      961 gagttgcact gagcatacga agctcgtcga tttttgagag aaatgcaatg catttttcat
     1021 tgccggtgtc ggcagctttc cttatgcaat gatagatcgg ctttttgcgt tcgtcaattc
     1081 gcatctgagc cagctcgtcc gcagtgaagc cgaaaatcgg cgagagcaga attgccagca
     1141 gcggaacgtc ctgaatggga ttatccgtca ctctcattag actcagcatg gtgctgattt
     1201 ctggcgaggc gaaaaatccc gacttgcttt cgatatatga cggtatgagg ttttcggcaa
     1261 gtatcttttc gtatatttcg gcttttccgc cgcctgccga gcgcagaaga atgcaaaagt
     1321 cgcggtacga cgcttttctg tagccgtttt tatcctttac cttcatatcg cccgaaagaa
     1381 tttcgtgtat cttatcggca atgaactgcg cttcgctttc tctgccgact ttttccgcca
     1441 tatcgccgag aatatacatc tcggcttctg ggaagctgct ttcttcataa tccgccgccg
     1501 caacaagctg ttcattctcg ttgtaatcga ccgaacccat ctgccttgac attatctgag
     1561 aaaagagaaa gttaacattt tccgtaacac ccgaacggct tctgaagttg cggtcaagcg
     1621 taatttttgc aggatagttg tcgcactcaa agtcattgag agcgtcacgc cgtgagagga
     1681 aaatttcggg cattgcctgg cggaaacgat agatgctctg cttgacatcg ccgacagtga
     1741 acatatttgt ttcatccctc gaaacagccc aaaacagagt gttttgcaag tcgttgacat
     1801 cctgatactc gtcaacaagg atttcttgga aacgctcggc gaagctttcg gcaagcgctg
     1861 tctttccggc gttgccgctc tcgtcgtatt tcacaagaag cttcaacgca aaatggcata
     1921 tgtcggagaa gtcatagctg tttgcccttt ttttctcctc acccaggatt tcgccgtatc
     1981 gcttaaccgc ttcaatgagc ttttcggcaa cagggcgaag ataatccatg tcgtctttat
     2041 tctcactctc agacgcacag aaaagcggcg caagcttctt caattgatcg gtaagaacat
     2101 ttttctgtgc cttgaccgat tcggactgga cagaggaata cttgcttggc aagcggccga
     2161 gactgccgac agaagctgag gtgacagcac tgcgtatctc gtcccatgag ccgttctcaa
     2221 tcaccttgga aagctcgtca aacattacgg cgttgttggc gattccgact ccgtattttt
     2281 cgccgacaac gggatcgtcc ttgcccgatt caatcatttt gccgcagagc atacggcaat
     2341 attcaaccga ctttgcggcg tagtttatga taattttgcc ccacacttga tcacgcaggg
     2401 gagcgtcggt atgatatgcg ctcgtaagac tgtcgagaaa ggcttccggc cttgcaaaag
     2461 ccattgaatc gttataaagc ttgcatataa ggtcggtcag cgcactgtcg tccgtgccgt
     2521 ttgccattat gcctacaagt tcgacgaatt cgggcgaatt ttctctgtaa agctcgtcca
     2581 taacctgtga aacggcgtcg tcacacatca gggcaagctg tgaattgtca agaatcccgt
     2641 agtcggggga aatctctata tcgtgaaaat tctcacgaac aatgtcgtta caaaagccgt
     2701 cgatagtact tattcttgca aaggggagca gaagcgcctg acgcttgagc agatcgttat
     2761 cgggagcctt gtcgatttcg gacatgagcg cctgatagat tctgtctttc atctgtgcgg
     2821 ttgcggctct ggtgaaggta acgattaaaa gcctgtcggc aggacaaggc tctttttcat
     2881 ccatgagcct ttcgataaca cgctgaacca aaacagccgt tttacccgag cctgccgcgg
     2941 cggagacgag aatcgagcct ttgcgcgagt caatcgcttt tttctgttct tttgtccatt
     3001 caggcattct cnnnnnnnnn nattctcatc acctcctaat ttctgcaagc tttcggaatg
     3061 gttcgggctt gttatctctc tcgtcggcgt attttcgtca acatggcaaa cgtccttgta
     3121 gtcgcagtat ttgcaaatat cgttgtaggg actttgactg ttggctgcat gcgccggcat
     3181 aacgtgaata attccgttgt ggagatcaac tgccattttt tcgagtattt tttcacatcg
     3241 cttcatcaga agctccattt gttttattga aataagattg ccgccgttcg tgccgtcctt
     3301 cttgattgag gcgggcac
//
LOCUS       DAWBSW010000208         3311 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4726_length_3311_cov_2.273034, whole genome shotgun sequence.
ACCESSION   DAWBSW010000208 DAWBSW010000000
VERSION     DAWBSW010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4726_length_3311_cov_2.273034"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    632..641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          854 a          769 c          771 g          907 t
ORIGIN      
        1 gagtaatgca aaggggagag cctgtcggat aaacgggatc accgggttta agtccggagg
       61 tttcctcgta gacctgaata acagtctcgt cgctgtcgat tctgataacc tcgccgataa
      121 ggcgctgatt gccgacaaag accatttcgg tcatttgcag gtcggttttt cctttaacag
      181 aaacaacagg accgtttatt gaatatataa cattttccat tatgtttgct ccttctgtca
      241 aagagataag gataagccgg agtagctgta aaaattctgc ttttccgttt caaggcgtgc
      301 gtcaagcgta tcgtcggcag tcattgccgt gcgaagattt tcgcccttgc agccgccgag
      361 ctttatgaat tcatcatatt tgacctcatt gccgagagcc tttatctcgg cttcaagctt
      421 tttgtcatca ggcctgagaa taataacggc gtcgtcaccg attgcagcct tgatatttga
      481 tacactgtta attagaaaat cgcggtagct gtcactcgct gcaaaagcct tgattttttc
      541 gactgcctga gcaaacacct tctcggttat ttcacttcgg cgtgcaacaa gcttttcgat
      601 ttctttagtc tcattttcgg acagctcggc gnnnnnnnnn ngctcggcgt tgatttcttc
      661 gttaagacgg tcaaattcac tgctttttac cggacggaca ttctgacggg caagacggcg
      721 agtctttttt atctcgttct cgatgtattc gtcaacgtca tttttaattt tttcgcagcg
      781 tgcatcggcg tccgaattta ttgccttgag aaaacgctct gttttacttg aattgttgat
      841 caaattcatt cctccttctt ggagttttga aagttatatt ttaattccga tcgcttcgct
      901 tacatagtcg gcgatcgagt tgcttgtacc gtccgagtgt ctgtcgggga tctcgattat
      961 cagcggcagt gagtgagcct tgcgaaatgc gccgatctcg tccgagcaga gtgatacaag
     1021 cgtttgattg atgaggatta tgccgatgtc gggatcctta gcagcctttt caaaaagctc
     1081 gcgaacctta tctctgctct ccgttacggt gccttcaatt cctgcaaggc gcataccgag
     1141 agccgtatcc tcattgtcac aaatcagata aagacgcata agctcacctt agatcttgtt
     1201 aagaataagg attgaaataa caacgccgta aagggcgatt gattcaccga gggcaacgaa
     1261 gataagagct ttaccgaatg ccttagcgtc ctcacttgtt gctgcgatag ccgccggagc
     1321 acctgccgca acggcgatac cgccgccgac gccagaaaga cctgtaacga tagcggctgc
     1381 aagaagtccg agacccttgc cgttatctga atttgatgct gttgacgttg cttcctcgct
     1441 ctgagaggtt gttgtagtct cgtttgtctt gtcctcattg gctgccgaaa cagagaatgt
     1501 aacggcgcaa acggtgattg cgattactgc gaaagcgatg aggtttctgg cgagaacctt
     1561 cttgccggaa acacctcttg acttgcggtt aaaggaatat gcaaaaagtg ccgtgagagc
     1621 ggcgatgggg agtactgcga taagaacata aaacaatgac atggttatta cctccgaaaa
     1681 ttatttttta actgttataa ctacgttttc gttgtcctcg gcatttgagg gcttgaaagc
     1741 gtttttgatg ctgaaaaata cacctttgtt tttgcgtgtg ccaacgctgt tgaacggctt
     1801 gccgtcgccc gcatagaatc ggctgaacat ctcatagaat tcaagtctca gaacctgaat
     1861 acccgtcaga agtccttcga gagcgataac aatgatgttg ccgaatatca tcgagatgat
     1921 accgccgacg gaatattcgt tgcctgccag cgatgagaaa accatcatca tgccggcatg
     1981 gacgagaacg aatgcaccga ctctgaggaa cgaaacggtg ttgctgaaat aggtcaggat
     2041 atattcgata acctcaaaga agttctgcat gatgtagtcc gaccatttct cgggcatgta
     2101 gttctctttt ttgtccacca ttccgataag aatctcctgg aagaagagaa gcagtatgca
     2161 aacaactgcg acgattacga caactcctgt cgggagcacc gctttgcctg ccatgaagcc
     2221 gactaccgca catacaagcg ttgcgtaaag aattataccg acaacgccgt tggtgctgaa
     2281 cagcgcttcg ccgagctttt tattcttcag cgagcagaaa acgttcagac ccatggagat
     2341 cacaacaagc gcaacgccta tgccgattgc aagaatcaga acggtgttta tcgaatccat
     2401 gaccgaaatc ggcttaccct gcaatccaat tgcatgataa aggggatcga gaagctcttc
     2461 gtaaccaaac acagagccga caagcagtcc gaaaaatgcc gagctgatgc cgcacggaac
     2521 aagaattttt gccagctttg ctcctttgat tctgtaaaca agatatccgc cgatcgcaag
     2581 cacaagtccc tgacccagat cggcaaacat aatgccgaaa agaagggaat aggtgattgc
     2641 aacaaaaggc gtgatatcaa ctccgccgta ggagggaagt ccgtacattt caacgaacat
     2701 ttcaaacgga cggaacggct ttttgttttt gagcttgacg gggggactgc ttcgcttctc
     2761 ctgctcggga tcggcatcct caaaggtaac tccgtcaatt ttttcaatat tctgtttaaa
     2821 tcttttgtag cttgatttcg gaacccagcc gacatagatg aaatagtcgg agtctgaatc
     2881 ctttgccgcg taccgtctga ggtcaaaggc aaggcttgcg acctcaagat aggcatgaac
     2941 ctgttcgcag tgttcggaat tctctgccca gaagtctgca atttccttat caatcttgtt
     3001 aagctcggtt gtgagttttt ccttttcggc atttagcttt gcgagtattt ctttcggtgt
     3061 gcccgttgag aaaggaatac gaattctgtc aaaatacagc gaagcaaaaa ttctgtcaac
     3121 ctcgtctatt tgagttctcg gagccgaata aataccccaa tgactctttt cgtcaacaga
     3181 cgattcaagg aaaacgaaaa ggttgttgct ttcgtaagcc ttgagcttcg ggtaacagtc
     3241 ttttggaata ctgccgaagc gaaccttcaa gaattcgcag gaaagaagtt cctcaatcgg
     3301 aatgtcaatt c
//
LOCUS       DAWBSW010000209         3307 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4732_length_3307_cov_2.535978, whole genome shotgun sequence.
ACCESSION   DAWBSW010000209 DAWBSW010000000
VERSION     DAWBSW010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4732_length_3307_cov_2.535978"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1016 a          742 c          643 g          906 t
ORIGIN      
        1 ccgctgtgca catgccgtgc ttaatgacga gttctgagta aaaaaagaaa aaagctatag
       61 ttcacgaccg tcggcagata ccgacactga actatagctt tattttttat tctatgtatt
      121 ttcttcttct ttcttccgca gataaattgt aaaaaccgcc tgagagagac ccagaaacat
      181 catcatatat tgaagcggct tgagcccgta aaacaggcaa tctgtcattc cacaaaggag
      241 aaatcccgaa acacttgcaa aaagagtaac cgcgacattc cttgtgcctg agccgagttt
      301 tgcgagccga aaaatattta tgtagaatat cacaaaaatt gccgcaagca gagttgctcc
      361 gaaaactccg ttttcaagcc acgtttcaag aaagatattg tgcgcatgag gctgtctgac
      421 atgatacact tcatgcatta cacgacggat attatcaaat cccgttccgt atccgaaaat
      481 cggtttttct ttaagcatcg caaaagctgc gtcccaaacc tgctttctcg tgtttacgga
      541 aatatccttg ccgctgatga gcgtgaggag ccttttgaaa ataccgtttg caaaaatcgc
      601 aatggcgcaa actccgactc cgccgagagc cataagcttt aagcctttct ttccgccgta
      661 gagaagcaag agaaagaaaa cgatcaccgc aaagagatac ggacctcttg aataagaaag
      721 cgcaatgccg ccgatggttg taaggcaaca aacaagcccg atccatctgc gctttgaatt
      781 ttccgagcaa aggaaaacat acgccgtaaa cggaagcata gaaacgcaga aggttgcaaa
      841 aaacagcgga ttggaaaatg ttccgcttgc tcttgttaaa aagaaatgga actttgttga
      901 tgccatgttt gaaacaataa aatccggcag atatttaacc agtctttcaa caccgaaatc
      961 aagaaagcgc caaaaaggat tgaagaattt cgcaagatcg cttcccgtcc tccgacagag
     1021 atggtaaagt accatttgtg caattccgat aaatccggca attcccgaag acagacagcc
     1081 gaaatagaac accctgtcaa cagcttcctc ggaatccacg gtttcggtga aaacatagta
     1141 tcccgtaaac atcaggcacc ataagccgat gcttgccaaa gaagagattg ccgaggacga
     1201 ataacttgag cagatcacca tccacgccat gtaaatcaat atcgaaattc cgagtttgcc
     1261 gaaattcggt ttgctcttct ctgctattct ttttttcaaa acaagagcaa aaatcacaat
     1321 gcccaaaaca ggtgcaaagt attcaggcaa cagtgccgcc aaaaggaaca aaagcggagc
     1381 ataaatatag cttttatccg attcgacagt taattttttt gttttcaaga atcagcttcc
     1441 tcctcattgg ttttgtcggc ggtcccttct tcgttttcgt cgtttttctt ctttcctttt
     1501 tcgccgataa tctttttata aaagaacgaa catattatgc tgagaagcgc agtaacggca
     1561 aaaattataa ttgcaagatc aaagtttctt cccgttattt ggtttccaca ccaggttgat
     1621 gaaatgattg acggaattct tgcgatgctc gtaatcagca tgaatttttt catgtcaaga
     1681 tccgttattc cggcgacata agtcaataca tccttgggcg ttcccggaat aagataaata
     1741 acaaaaagcg ttttgtacac cgttttttta tccttgagga atttcaatga gtcgattttc
     1801 tcgatcggta taaaaagatt gacaagacgt ctgccgaaaa gctttgtcaa aagaattatg
     1861 accaacgagc cgatctctgt tccgagcaag cacaggaaca tacccatcca tgttccgtac
     1921 aaggcgcccg aaccgatttc aagcggctcg gcaggtataa ttttgacaac ggtctgcatc
     1981 gcacgaatcg caacaaaaac aagcttgtca aagcctttaa aacgagacag aaaggctttc
     2041 atgtgctcgg catcgcagag cagggaataa agctccttac cgtactttat atatatatag
     2101 ataaaaactg cgactatgac agagcaaaca acaattgacc ccaccctttg aatcaggcga
     2161 agcttttttt gctttgcaac atcagagtca cagcaatttg tatttttcaa aaaaatcacc
     2221 gagccttccg gtgtatttct gttctttcca aggctttttc ttaccgttga aatgaataat
     2281 aaccgtattt ttctctaccc agtcaaggtc gatttttctt tccccggcaa aacaggaaga
     2341 aaaatgacgg taggtttttt catcaagatt ataaactctc tcgtcgatcg gaagcgtgcg
     2401 gtttttcatc aaagcattta tgacatcctg atcggcaaga agaagctttt tatggttgac
     2461 tgagataaat ttaaaaatcc tttttaaaga aatctcttct ctccattttt ttaaatcaat
     2521 cagcaacacg ccggcattaa tatagcggct tcctttgctc agaccgaggc gtaagcgatt
     2581 gaccccctcg gggattccgt aaagatgcgg agcacctgca ataatattcc cctgcaaatc
     2641 gagattataa aaatcactca gatcgccgtt gataacgata tcgggatcga gataaagaac
     2701 tctttctacg tcatttggga gaacatctcc ggcaagcaga cggtaatatg tcgccttgct
     2761 tattctgtca agcacaggac agccgtcaaa catatcgtcg cttacgggga ttctgtgtat
     2821 ctcatggttt gtcatgccga gcgccttttc catgtccgaa aaatcatttt cggtcagcga
     2881 tgaataagca acataaagat caatccgatt gccggaatta gtttcggcta tcgagttgag
     2941 cataacgcac agcggatata tgtaatttga atcaagcgtt ataaaaatat tcatagcttt
     3001 cttcacctgt cagacacttc atgtgtcaat tatagcaata aaccccgtta aagtcaatat
     3061 ggacatattt gctttttaag cttagataca ctgtctttaa tttctattat atttttttat
     3121 aaaataaaat cataaaatat atcgttattc ttgaaaaaag agccgccgaa gcgactctga
     3181 tcggtgtggg aaaagatact tgacgtctgc tgcggctgtg ccttgcattc taacagttcg
     3241 ccgaccgtcg caagcgacag tacccggctc accgctcctc gctaaaaaca gtccacagga
     3301 ctgtttt
//
LOCUS       DAWBSW010000210         3304 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4737_length_3304_cov_2.602031, whole genome shotgun sequence.
ACCESSION   DAWBSW010000210 DAWBSW010000000
VERSION     DAWBSW010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4737_length_3304_cov_2.602031"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    648..657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          939 a          752 c          703 g          900 t
ORIGIN      
        1 gcaaccaaga gcgccgagat tgagaaggca tgggaccgtg tgatgttcaa tcagttccat
       61 gatattcttg ccggctgttc aatcaaacct gcctacgatg atgcttacgc aggcttcggc
      121 tatgcaaaag aagcggcaat ggagatcggc acttttgcgg cggagcgaat ttcatggaga
      181 ataaatacaa ccaagttttt tgactccgag ctttccgaaa tcagaggacg gctttggact
      241 cgaagcggcg agggctcccc gatggtcgtt ttcaatcccc attcgttccc cgttaagggc
      301 tatgcaatct tcggcgagca ttgggcgacg ggagttgtcg atgagaatga caacgacgtt
      361 gaatttcaga tgataagagc gccctatacc gacggccaaa gccgcaacag atgtctcttt
      421 aacgttgaaa ttcccgccta cggctactca acctatttca ttttcagaga caagcagaat
      481 tacgttccgc aggagagaga aaatcaattt gcaatcggcg aaaagttcat tgaaaacagc
      541 gttgttcgcc ttgagtttga cagcgacgga cgcataattt cttatttcaa caagaagaaa
      601 aataaagaat tctgcgaaaa gccgctgagc gcaattgtct gcgaagannn nnnnnnntta
      661 accaggacgt cggcagcttc ggcaatgcgg agttcacagt tgaggaaaca ggcactctcc
      721 gtgcaacact gagagttaaa tcgtattata ataactccgt gctcatccaa tattacacgc
      781 tctacaagga cagcgacaag gtcgaggtca gatgcaagac ctcgttcaag gaggaataca
      841 aaatcctcaa gctcgccttc cctgttgcga taagcgaccc gacggctgtt tattccatgc
      901 cgtttggatt catcaagaaa aagcccaacg gcgaggagga gcctgcacag ggctgggtat
      961 cgctcgaaaa cggcacggac ggctttgcgc tcatcaataa cggacgttac agcttctgcg
     1021 ctataggcaa cgagctgaga atgattgccg ccagagcgtg tgcatatctt gaccattacg
     1081 gtcaggcaac ccgtgacgaa aacatggaaa tgcttgacag cgacgagctt gaatttacat
     1141 acgaattcat gagctgcgac agcggaaatt attccgagct agtccgtgag gcagaggtac
     1201 tcaacatgcc gctgaagctt tatcaggaga cgcaccacaa gggcacgctt ccgccgacct
     1261 acagcggaat gagcattgac atcgacaatg taatcgttca gacaataaag attgccgagg
     1321 acaataacgg atatgttctc cgtgcgattg agacgggcgg ccaagcctgc acggcaacgc
     1381 ttgatttgaa gttcatcggc agaaaaatca ccctcgattt taagccgcag gaattcaaga
     1441 caatttttgt tccgtttgac ggtggagaaa tcagagaaat gttactgaca gaacttgact
     1501 gaaaaatcga cctccttgca ttatgcaggg aggtcattca tgttttatgt caccacttaa
     1561 ttaaaacaca gccgtttttt acaccgaccg tggaggtgtt ttctttaaat ttattgctca
     1621 cgccaagcgg aaaaaacggt gtaagtgtaa gattttccgt ttcataaaca gcacctttga
     1681 ctgatattcc ctcggctatt ccgccgtagg cgaaaacaga aagcgttttc cccttttccg
     1741 agcggagctt gatcgaggaa tttttaataa gcgcaaaggt ttcgttttcg ccgacaagaa
     1801 ttccctcgcc gccgtggtca acaatatacg ctaacgactg aatgtttgca atcgtatgat
     1861 ccgttcgctc gccgccgaga ccgccgtaaa tctcaaagcg tttgtagccg ctgttgaagc
     1921 cgtactttat cgccagcatg gtgtccgtat catccttgag aatcggaaag gtttcaatcg
     1981 gtgtatcggt gttcggcttc tttgacgaat caaaatcacc gattataacg tcgggtctta
     2041 aacctgcctt ttgaacattc aggtatcctg catcagccgc aattataaga tcgtccttat
     2101 cgggcttcgg aaaattattt ttcatctcaa ccgcagagaa aatataacat gttttcattt
     2161 cttcaccatt gttttcaatt tatgtatata ttataatata aactccggtt tttttcaaca
     2221 tatttaaaat atgacatcac ttcgcttatt tcctgtatct cttcagagaa tacagtttta
     2281 aaataaaaac cgccgtattg ccgatatata caatactctg tcaaaaaaga cgaaaatcaa
     2341 gtttttcgct gattttcgtc tttctttgtt ttattttgtc ataagctctt tttgcttttc
     2401 ggatttttct tcaatctctt tgttcacgtc atattttttg ccgtgatgat acgggaatat
     2461 cttgaggaat atccatgcag caactgcacc tattgagttg agaattatat ctcccatggt
     2521 atccatgatc ggccatcttt cgatattttt aggatcaaag atcgttgatt cctttgaggt
     2581 tccttttgca agctcatagc accaatgctg agcgtcaccg attgtgccct tttcggggtt
     2641 tgtcatgacc tgatccattg tgaactccac aagctcccag caggtcgaaa caaagaagga
     2701 aaaacaaagc gcacagagca aagcaacggg aatcgaaacg ggcttgttgt ctctcttttg
     2761 cattgcaacg acaacctcat agccgagtaa aacgcaaagt ccgccgctga gaagatgcat
     2821 tcccgaatcc cagaatctcg aatcataata gaagttaagg aactttccgc agaacgatgc
     2881 ggcaaaaatc ataactattg aaaaatcctg tatcagtgaa ggcatatgtc tcacaaagca
     2941 tttttcggga aaaagcataa atatttccca agcgaacatt gcgataaagt ttgcgccgac
     3001 ctgaagcgga tccgaaatat caaacggctt tctgaaaaaa cctgcgataa aggcataaat
     3061 cataagtgct ctgaaaatcc accagaaaac aatttcccat gtcttgcaat ttgactttat
     3121 acggccgata tattctttca ttacatcacc ttttttaatt tgttaacacg tgcgttcttt
     3181 cataatacga caaaacaatg tgtttgtcaa tatctattca agctcgtcct cgtaatcttc
     3241 cttggaaaag tcgaagtttt catctatctc cactctgccg aattttcttc gtttggaata
     3301 atct
//
LOCUS       DAWBSW010000211         3295 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4756_length_3295_cov_2.539198, whole genome shotgun sequence.
ACCESSION   DAWBSW010000211 DAWBSW010000000
VERSION     DAWBSW010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4756_length_3295_cov_2.539198"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1118..1127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1635..1644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1997..2006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          928 a          702 c          647 g          988 t
ORIGIN      
        1 aaatcggcct ttccgacgga ttatagtttt caatataatc aagcagatcg gaaggggagt
       61 cggacatata gtaaagcttc ttacattcct cattcataaa gtgattgtcg attccgtttt
      121 gaatgaactt ttgcagactg ttatagtaac cgttcgtgtt gagaattgca atcggtttgt
      181 tatgacggtt gagttgttta agcgtaagta tttcaaagaa ttcgtcaaac gttccgattc
      241 cgcccggagt gatgatgaaa gcgtcggaca gctcttccat tttcatttta cgctcacgca
      301 tatttttggg atatataaac tccgtgcaat gctcgtagag tatgccgtct acgttgaaaa
      361 attcgggagc aattccgatt atttttccgc caaaacggtg ttcgcctctg gcggccgcgc
      421 ccatgagtcc gtttgcaccc cctccgaaaa caagtccgat acttcttttt gccatttctt
      481 cgccgagctt ttctccggcg tcgatatagt attgttctat cgttgtgctc gatgcaccgt
      541 aaacacatat attcatattc ttctcccatc aaaagttttc tttaaaaaat ttttctattt
      601 cttctttaga agcctcggcg ctgccctgta aaagctccga ggagccgccg ccgcgaccgt
      661 ttaaggctga ggtaatttct ttcgattttt cacgcaggtc aatgctttta gaagcaattg
      721 caaagcggta tccgtcatta ttattgcctg aaaaaccggc acaaagcttt tcggtttttt
      781 ctgcgccgtt aagcacaagc tttcgaaggt cgttcatgtc cgtatccgct tcaaaaatca
      841 aaatacattc gtccgtattc tccatagccg aagccttaag ctgtgcaagc tctcttttca
      901 gcgcggcaag ctcgattttc aatgcactgt gttcttcatt aaatctttca aaagcctgcg
      961 ccgtttcatt ttgctttgcg caaagcacgg ttgcgaccga gcgaagattt tcatacttct
     1021 tttcgtaatc ctcaagcgca tccttgccgc aaagaagatg aacacgtatt ccgcctctgt
     1081 gacgtgcaaa gtcaagaatt ttaattattc cgatttcnnn nnnnnnngcc gaataaacat
     1141 gcacagcaca gcaggcgcaa acgtcgtatc cctcgacagt cacgattctg acattttccg
     1201 tcagctcaag cttgcttcga tagctgagat ttttcagcgt ttcttcatcg ggatattcac
     1261 aggtgaattt tgcatttttg taaatagcct cgttagctct tttttcaaca tcaatcagtt
     1321 gttctcttgt aagctcgccg tcaaagtcta tagtcacgtc gtcgccgaga tgaaagccga
     1381 cattctcata tccgtaaagc ttatgcacga taccggaaac aatatgctca cccgaatggt
     1441 tctgcatacg ccgaaaacgc tgttcccagt tgatttcaca gcagagttca tctcctatat
     1501 ttaatgccct gtcggcataa tgtacgatct cgccgtttat aatctgcacg tcgtttatat
     1561 cggcttcacc gattttgccc gtatccgcat actgtccgcc gccctcgggg aaaaatgccg
     1621 ttttgtcaag aatgnnnnnn nnnncaaaac cgttttcgtt tttacggcag tcaattacct
     1681 tcgcggtgaa tgccttgata tggctgtctt tatcataaag tttttctgtt atcattgttt
     1741 cactcctaac ctttagcatt gtatcacagt gcagaaaaaa atgcaattca ttgattgaca
     1801 atgacctgca ttatgataaa atatttccga ggtgatttga tggaatacac tcttgtaagg
     1861 tcaaagcgta aaactgttgc tttgcagata aatgccaaag gtgaactgat cgttcgtgca
     1921 ccgatgcttt attcggagcg taaaataaaa aaactcgttg aagaagcagg actcaactgc
     1981 gaagtatctc tcgattnnnn nnnnnncacc gtgaatggat agaaaacaaa aaagaggaaa
     2041 gcattgaaag aaaaagaaat caccccgagc tgtcaaagga agaaattgtc gcatacaaaa
     2101 agcaggcaaa agctctgctg ccggctttaa caaaaaaatt cgccgcagtc atgggcgttg
     2161 aatacggaac cgttaaaatt acatccgctc aaaagcgttt cggctcttgc agcgggaaaa
     2221 acaatatttg ctattcatat attcttatgc aatatcctat tgaagcgatt gaatatgtcg
     2281 ttgtccatga gctggctcac accgttcacc atgaccacag cagagcgttt tatgacatga
     2341 tagccgaata tcttcccgac tataagcaac gggaaaaatt gctgaaaaaa tgaaagcttt
     2401 tattaaagta taattttgtc gatctattat aaccgttttc agtgatagat ttttgcaaat
     2461 gtcatacatg actttattaa aaaaggctat gtcaaaatga atatatattg tgcttgtttt
     2521 tgtagttttt aaacaaaaag tacatatatg tataaaattt aattgatttt tacatttcga
     2581 tgtgataaca taataattgt tgtaataacg caattttatc aaggaggaat tgatatgaaa
     2641 gcaaaaaaag cgtgcaaaaa agcgttatcg atcgttttgg ctttaacgct tatgctcaca
     2701 acatttgttt gctttgatat cggagcgctt ttcggctcgg ctgttgatac aagctcggct
     2761 gttacggttt ccgataacaa tgtaagcaat gtgtactttt atgctccgga gcagatatat
     2821 cttgagccgt cattgacggg atatacagcc cagggcaagt acaagtatca gtggtttgtc
     2881 gattcaaatg tcgatgaatc aacaaaatta caaactctca gaacgggtga gaattcagca
     2941 ggtaattttt acttctatta tgagaatgca agccagattt caatttccta taagtatctt
     3001 aacaatgata tgacggatat gacggcttat cagaacacaa gcgcaactac cacaacggcg
     3061 gattatgcaa atgccaactg taatattaag cttgacgctt acgcaactcc tttgagagct
     3121 tccaacacaa gcggcacaag ctcatatatt tactacacgg ttgcgggaaa taagatcagc
     3181 acaacgatca ctcgcgagag tgagtctccg tatcttcttg ccgcaactaa cggctactac
     3241 attgagtgga aggcaacatt tgtcgataag ctcgacggtc agaccaaggt tgctt
//
LOCUS       DAWBSW010000212         3291 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4764_length_3291_cov_2.433251, whole genome shotgun sequence.
ACCESSION   DAWBSW010000212 DAWBSW010000000
VERSION     DAWBSW010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4764_length_3291_cov_2.433251"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          970 a          743 c          717 g          861 t
ORIGIN      
        1 gtatagcaaa caaatcaatt aaaaatctat tcacaggaaa ttcacatttt tttggtaaaa
       61 tttgaatatc aaaataaagg agtgatttaa atggcaaagg ctttgccgaa gccgattgac
      121 ggaacattga ggaagttctc tctgcgagtt cccgaggtta tccgtgagtg cagcggaatt
      181 gttgtttttg ggaaaaggat aaagtcgctt gttttttcaa ccgacgttgc cataatcaga
      241 aatgttaatg cagacgcgat aatcgccgta tatccgttca caccccaacc gataataacc
      301 caggcgattt tgtccgcttc ggacatccct gtctttgtcg gagtcggcgg aggtttgacg
      361 agcggaaaaa gaattctcag cctttccacc tttgctgaaa tgcagggagc gacgggagtt
      421 gttctcaatg ctccggtgtc aaacgaaatt gtgcgggatg tcagcgacaa tattgatatt
      481 ccgacagtca tgactgttgt tcgcgaggac gatgatattc aggctcgaat agatgccggt
      541 gcggccattc tcaacgtttc cgccgcttca cgcacacctg aaatcgtagc caaaatccgc
      601 aaagatttcc atgatatccc gattattgca acagggggtc cgacagagga ctcgatcaga
      661 aaaacaattg aggcgggcgc aaacgcaatc acatggactc cgccttcaaa cggcgaaatc
      721 ttccgtgatg tcatggatgc ttaccgagcc ggtcagccgc acccgtaaaa cgattataaa
      781 gaaggcgatt tttatgacgc tcaccgaagc aatcacggca agacattccg tgcgaagctt
      841 tacagacaga aaaattgatg acagcattgc cgccgagctt cagaaaacaa ttgatgactg
      901 caaccgtctg agcggcttta ggattcagct tatgctcgat gaaccgaacg ctttttccac
      961 acgaatggca cactacggaa attttcacaa ctgtaaaaat tacattgcaa tgataggtcc
     1021 aaaaggtcac gatgaggact gcggctatta cggcgaaaaa atcgtcatta aagcacagca
     1081 gctcgggctg aattcctgct gggtcgcagt gagctacggg aaatcaaagg tgccttgcaa
     1141 cattcctcac gggcaaaagc tgtatatggt tatcgccctc ggctacggaa caacacaggg
     1201 cacacagcat aaatccaaaa gtatggacga attgtgcaag gttgtcggcg atatgccgcc
     1261 atggtttttc aatgcaatga ctcttgcaat gcttgcaccg accgctgtta atcagcaaaa
     1321 gtttttgttc acccttgata acgatacggt tcgcgcaaag gctctcaggg gtttctactc
     1381 aaaaaccgat ctcggaatcg ccaaatacca ttttgaaata ggtgcaggcg atgccgaatt
     1441 taaatggtca taaaacttta tatttttttt tggagctgat agcgatttca gctccctttt
     1501 ttgttgaaaa aatctatcat atatgatatt atctatagaa gcaagcaaaa ggaagtttca
     1561 gcatgataaa aaaagttgtt tctgttttgc tctcgttagc aatcatattt tcggcagcag
     1621 gctttgctgc ctatgcggag gataaaaaca tgatttttgt ttcaccaagc ggcaatgaca
     1681 atttaagcgg cacaattgat gctccgcttg ctactctttc cgcggccaaa gaaaaggcga
     1741 aaaattttga cggcaaggtt actgtttact tcagagaagg aacatacaca attgagaaca
     1801 ccgttaattt tgatgccgac gataagtccg acgttgttta caaggcgtac cgtaacgaaa
     1861 aggtcatttt tacatcgggc agtccgtaca caggctttga tgagtgcaca gttaacggcg
     1921 ttcgtgcttt taaaaaggat atcggcaagg gtgcagattt caatattctt ttcaacgagc
     1981 aaaccacgct ttcaagaact cgctatcccg aaagcggata tttttatgtt aacgatgttt
     2041 ccgatgatga cattcagccg ggcgacgata aaaacgacca atatcatacg ggatttttgg
     2101 gtatgtacat tgataatagc aactttttcg atttcaagaa tatggaagat gttgaaataa
     2161 agcttcttca tttttggaag gatgaaacac ttttaataaa gtcctacgac aaaaagacga
     2221 gtcatatcgc ttttaacaaa acatctacca tgagaataaa caaaaatgac cgcttcttcc
     2281 ttcaaaatgt ttttgaaacg cttaacaaac ccggtcagtg gtatctcgac aaaaccgaag
     2341 gcgttctcta cgtcatcccc aacgaaagcg atacgcccga aaattatacc gtttgggggt
     2401 caacaacaga gactatgatc tccgtcgacg gtgttgacgg aatttccttt gaaaacattc
     2461 tctttcgtgc caaccgattt ttttatctcc ccaagcgtga attcactcag gcggcatacg
     2521 atgtaaaatc ctgtatctct tataaaaacg caaagaactt ccatataaaa aattgtgaga
     2581 tcagagacat cgctgcctgc tcggttttct tcggcagcaa tgttcggaac gcctcggttg
     2641 aaagttgcct tttcaacaat ataggtgcac aggctgtcta tgtccatggc gaaaacattg
     2701 acgttaacga cccgagcgtt accagaaata taaccatctc caacaatcta attaccaaat
     2761 acggcagaac ttatttcaat gccgttgcaa ttctcgttat tcatgcaaat tccgtcgaca
     2821 tctctcacaa tgaaattcac aacggatatt acacggcaat ttcagtcggc tgggtttggg
     2881 gctactccca caacgtttgc tataacaacc gaatttgcga caaccttata tacaatatcg
     2941 gtcaaggctg gctctccgac atgggcggca tatatacgct cggcaatcag cccggcaccg
     3001 tcatctcggg caacatcatc cataatgtcg ccgccgaccc ggacgaggga ggctacggcg
     3061 gctggggtat ttatcttgac gaaggctcat cgtatattac cgttgagaaa aatctggcgt
     3121 tttcctgcgg cagcaatgcg tatcatctgc attacggctc atataacacg gtgagaaaca
     3181 acatttttgt cctcagcggc gagtcgcaat tcaaaacggt gagcaatctc ggccgtgtaa
     3241 cgcccgatga cggcggcaaa aagaccattg accttttcaa caacattatc c
//
LOCUS       DAWBSW010000213         3266 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4807_length_3266_cov_2.523824, whole genome shotgun sequence.
ACCESSION   DAWBSW010000213 DAWBSW010000000
VERSION     DAWBSW010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_3266_cov_2.523824"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          786 a          832 c          668 g          980 t
ORIGIN      
        1 aaacatctgc tccctgcttt tgaccgacgg aaagctgcct aaaatcaata ttttgctctc
       61 acggtcccat acagggtcaa acgggtgctc aacatgtata agcgctttac ccatcgtatc
      121 acctcggtgt aattatattc ctttaatacc tgtatgtcaa agaatctttt tgttcttttc
      181 tattctcttt tatctaaaat aaataacccc tccgctttgc taccctacag tggcaccgag
      241 attaagcttc ttctgacatt gagtcgattt ggcatcaaca catatgaatt ttacatttac
      301 tgaatcttgt ttcatcaaga catattaaaa taaaaaaaca ccccgaaagg cattaagctt
      361 ttcggagtgt cgattacgat tgaattattc ctcaacggga gcttcttctg ctgcttcctc
      421 tgccggagct tcctccgccg aatcctcagc ggcctcatca gccttcggaa gaagggcacg
      481 gattgaaagg cttacacggc tcttatcgaa gtcgatagct gtgatcttgc acttaacctt
      541 ctcaccgatc ttaagaacat cagaaggctt gccgattctc tggtctgcaa tctgagaaat
      601 atggataagt ccgtcgatgc ccgggataac ggttgcgaat gcaccgaatg ttgtgagacc
      661 gacgatctcg gaatcaataa cagtatcaac cggatagtca cggcgaagga tctcccaagg
      721 attgtcctca gccttcttgt agccgagaga aatcttcttg ttctccttat caaggctctt
      781 gatataaacc tcaatctcct gaccgacgga aagaacctct gaaggatgct tgattctctt
      841 ccaagacatc tcggagatat gtaccattcc gtctacgccg ccgatatcaa cgaatgcgcc
      901 gtagtttgtg agtgacttaa ccacaccgtg gtaaacctgt ccctcttcag cctgtgccca
      961 gaaagcatcc tcagcagcct tgcgagcctc tctgataaca gccttgatcg aaccgatagc
     1021 cttcttgcgg ccgcgctcgg acttaacctc gatgatcttg agatcaacct tggtcttgag
     1081 aagatcgtca agagaatctc ctcttgaagc tgttgcaaga gatgcgggaa cgaatactct
     1141 tacgccgttt acaattacga taacgccgcc cttgacaacg tctgtaacag tacctgaaac
     1201 gacctcatcg ttctccttgg cagcttcgat cttatcccaa gcgccctgag catcgtatct
     1261 cttcttggaa agcataactg tgccttcctg atcgtttgtc ttcatgataa tgagcttgat
     1321 ctcgtcgccg atcttcagtt ccttcatcgg atctgctgtc ggatctgcgc tgtactcgtc
     1381 ggcaggaaca tatcctgtct gctttctgcc gatatcgacc tggatctcac tcggtgaaat
     1441 acctgtgact acaccgacaa ccttttgctc ggtgctcata ttgttaagag actcttcgag
     1501 cgcctccata aaatcaagat cttcagcgga agtttttgcc acctcctctg cattttcaat
     1561 cgggtttgtg tttacatttt ctgacattgt tgaaagtacc tcctttatga ttgccgcggg
     1621 agttgatgct cctgcggtga cacccacctt tcgacgtcct tggaagctga tatttttcag
     1681 ctcgtccgct gtttcgataa gataggtcgg gcagcagttt tcacacacag ccttcaactt
     1741 cactgtattg gaactttgcc gtcctcctac tatgatcatt gcgtcacatt cctccgccaa
     1801 attgatggct tcgtcctgcc gctcattagt agcattacat attgtatcaa aaattaaagc
     1861 ttttgtatag tcttttttta atttttttaa acatttttcc cattctaccg tattaaaagt
     1921 ggtttgagca accgctatta ttcttttttc gcagtttatc tcagcttttt cgagatcctc
     1981 aaggtttttg aaaacaacaa cctcgccctt acagtagctt tttattccct gaacctcggg
     2041 gtgatttgcg tcgcctgcaa tgagaacgat tgaattatcg tccgaattgt ccgtaacgat
     2101 tttatgtatt ttctttacaa aagggcacgc ggcatcccag tattcatatc ccctcgcctt
     2161 taattcgtca atgacctgct tttcaacgcc gtgggttctt agaaccagct ttgtatcttt
     2221 agggatttca tcaattgaat ttgcaataat aacgcccttg cttctcaggt cgtcaataac
     2281 ctgcggatta tgtattatcg gtccgagagt gcataccttt tcgccgcttc ttaccatctg
     2341 atacagccgg tcaacggcac gattcacgcc gaagcagaag ccggcggttt tcgctaactt
     2401 aatctgcatg gcctaacccc caaagctctt ttattctgtc cattatgaga gccgagccgt
     2461 aacggagatc cttcatcttg tcaacatcgt cgtggaaatc aagctcctca tattttatcg
     2521 gcttgccgta gcggactgtc agtcttgttc cctttttggc ctcgtcgctt gtgtagatcg
     2581 aaaccgggac aacgtcgcat ttgcattttt tcgcaacata gcagactccg cccttggctt
     2641 catgcggctt aaaatcatgc gagcgtgtgc cttcggggaa aatgccgagc gctccgccgt
     2701 cctcaacaac cttgacggca tgattcatcg catgaaaatc gaacttgctt ctgtcaacgg
     2761 gaaaagcatt gagattggca agaaaccagc cgacaatatt cttttcaaaa agctcctcct
     2821 tggcgacaaa atgaaacttc accttacagc cgacgccgat aataatggga tcgattgccg
     2881 taatgtgatt ggacgcaaga ataatacccc tgtcggtagg aacgttctcc acgccgacat
     2941 aggttatttt atatcttgag cttacaacta tctttgcaag aggccttatg atcttataaa
     3001 ggaagtggct cttgtcagat tcataattgt atttgtatgc cataatgacc cttccttttt
     3061 agtggtgtgt gaaatattct tgtcgcccct ttaggggagt gacggagtgg ttaaagtttg
     3121 ataaagcttt actccctcag tcacagacaa gctgtgtcca gctcccccga aggaggagca
     3181 cacgctcaaa gcaaattgta tctgactttt tatcagcctt ccccttgagg ggaaggtggg
     3241 cggcggattg cgccgctcgg atgagg
//
LOCUS       DAWBSW010000214         3258 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4819_length_3258_cov_2.517328, whole genome shotgun sequence.
ACCESSION   DAWBSW010000214 DAWBSW010000000
VERSION     DAWBSW010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4819_length_3258_cov_2.517328"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1016..1025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1330..1339
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          787 a          762 c          811 g          878 t
ORIGIN      
        1 aatgactttg agccttgaga agtcctcacg ctctttttcg tcaagcgcat cggaaatgaa
       61 cttgacggtt gatgtgaatt tcggaatcat aacgttgctc agggcatttg tacgcttctg
      121 agttttctta attgcgtcgg caagacgcat aacattgctt tcgatttcag caagctgaac
      181 cgtcagcttt ttgacctctt caaagttaat atatgcctcg tcaagcaagg aattgcttgc
      241 attaagaccg tagtaaaaat atctgttgcg ttcgtcgagc gtgatctgcg gcagctcgac
      301 gcccataacg ctacggacgg tcatgtgcag accgttatca acgggaatac attcggcaaa
      361 ggaggaacag ttgccgattg atatctgcgc ctgctcgaga gtgtaatacg ccttggcata
      421 tgccgtattg atctcgctct gaatggttga agctttttcg ctcatcgtca tgatctcacg
      481 gataagaatg tttctttttc tgtccatgag gtcgtagccg agcttagcaa gctccaacga
      541 tttcttcgtg gcaatgagat tgtttttggt cggaaaaacc tttgaactca tcggctgttc
      601 tcctttcgta aaattaagac ggattattta cccttataat atttctcaat cgtcttatcg
      661 tcaacacggt cgagatcctc tctgggcagc tcggaaagaa gctcccagcc aaggtcaagt
      721 gttttctgaa tcgaacggtt ttcgtaaggc gactgtgaaa tgaactttgc ttcaaagtcc
      781 ttgccaaact gcaaaagacg cttatcggaa gcggaaagtt catcctcgcc gataacgctt
      841 gcaaggctct ttgcatcctg aaccttagcg taggacgaga aaagctggtt tgcaagtatg
      901 ctgtgatcct cacgggtgaa gccctcgccg atgccgtctt tcataagacg tgaaagcgac
      961 ggaagaattc cgatcggcgg ataaattccc tttgaatcaa gctcacggtc aagaannnnn
     1021 nnnnnagaac gatctgaccc tcggtgatgt atcccgtaag gtcgggaatc gggtgtgtga
     1081 tatcgtcgtt cggcatggtg agaatcggga tttgagtaag tgaacccttt gagccctcaa
     1141 taattccggc acgctcatag agcgacgcaa aatctgaata aagatatccc gggaagcctt
     1201 ttcttgcagg aatttcgccc tttgacgagc tgaattcacg gacggcttcc gcatatgacg
     1261 tcatatccgt caaaataaca agcacgttct tttcaagcgt gaatgcaagg tattctgcgg
     1321 cggtcagagn nnnnnnnnna cgttcgcttt ttcaaacgat gatcggaaat agcttgcaac
     1381 gtcgttctta acgcccattg cggcaaaaac gattgcgaag tcctcactgc ctgagattga
     1441 ggcctgacga acgatctgaa cggcaagttc attgtgcttc atacccgagc ccgagaagat
     1501 gggcagcttc tgacctctga taagagtcat gagcgcatca atcgaggaaa tacctgtgtt
     1561 gatatagttc ttgggataaa ctctagaaac ggggttgatg ggcgacgagt taacgtcacg
     1621 tttttcaacg gggtaaacct caccgagtcc gtcgatcggt ctgccgagtc cgtcaaaaac
     1681 tctgccgacg atttcgggag aaaggctcaa ttcaatcggg tgacccgtca ttctcgtctt
     1741 ggtgttgacg agcgaaagac cttttgtacc ctcaaatacc tgaataacga ccttttcgcc
     1801 ctcgatttct actatacggc ctgttcttga tgagccgtcc tcaaggcgaa gctcaaccat
     1861 ttcatcgtag gaggcgtcct tgatgttgtc aagaacgata agcgaaccgt tgatctgact
     1921 taaaccgatg tgttcaataa tcattttgcg gctgcctcct ttgcactgac agaggcaaga
     1981 gcagcgtcga tttccttata gtattcgtcg aacatatcaa gcctgtcatt aggtatatca
     2041 tatttcatct gaacaagttt gtcaaataat ttaagctccg ttacctttga aagaggaatc
     2101 ttgagagcaa cgatttcatt gcttctgtga tagaggtaaa ggattatctg catcattttg
     2161 agctgctttt caagggaaac gtaggtgtcg tccttgtgga aagcgttctg ctgaaggaag
     2221 ccgacacgga taactctcgc aacctcgatg ataagcttct gatcgtcggg aagaacatcg
     2281 gagccgatta gctttacgat ttccataagg cttgtttcct cggcaagcag cgatgcgatt
     2341 tcgtttctgc aagtcataaa gtccgagccg acgttcttgt tgtaccacgg cgcaagctcg
     2401 ttaagatatt cgctgtagct gtcgttccag ttgattgcag gatagtgacg tgcgtaagca
     2461 agcgacttat cgagtgccca gaagcagcgg gtgaaacgct ttgtgttctg agtaaccggc
     2521 tctgagaaat ccgaaccctg aggtgaaaca gcaccgataa cggagattga accgtcctgt
     2581 ccgttgagcg tttcaacata gccggcacgc tcatagaact cggagagtct tgacggcaga
     2641 taagccggaa agccttcgtc ggcaggcatc tcttcaagtc gtccgctgat ttcacgcaga
     2701 gcctcagccc aacgtgaggt cgagtcggcc atcattgcgg tgtggtatcc catgtcacgg
     2761 taatattcag ctatcgtgat acctgtgtat attgaagcct cacgggcggc aacgggcata
     2821 ttcgatgtgt tcgcaatgag cacggtacgg tcggtgaggg gtctgccgga tttcgggtcg
     2881 gtaatttcgc cgaattcctt aagagcctgt gtcatttcgt ttccacgctc gccgcagccg
     2941 acgtaaatga tgatatctgc gtcgcaccat ttggcaagct gatgctgcgt catcgttttt
     3001 cctgtgccga agccgcccgg gatagcggcg gcaccgccct ttgcaagagg aaacagcgta
     3061 tcaattacac gctgacccgt gatgagcggc ttctcgggag gaagtctttt tgaaatcggt
     3121 ctcggtgttc tgataggcca tttgcggcag agagtaagct catgtagctt tccgagatcg
     3181 tcttttaatg tgatgacggt gtcgttgacc ttgtattttc cgtccggctt aacatcgaca
     3241 acctcgccgc tgagctcg
//
LOCUS       DAWBSW010000215         3218 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4882_length_3218_cov_2.356623, whole genome shotgun sequence.
ACCESSION   DAWBSW010000215 DAWBSW010000000
VERSION     DAWBSW010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_3218_cov_2.356623"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2871..2880
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          900 a          722 c          748 g          838 t
ORIGIN      
        1 ctgctcgtcc gtttcaaaca ttgcggccga cattctcttt gtcgcaggct tcaaaatggg
       61 cgtcagcggt gttgcatggg cgacctttat ctgtcagggc gtaagctgca tactcgccgt
      121 attcttcgtg ttccgtcgct ttgcaaatat tgaaaccgac ggcaagcctg ctgcattttc
      181 atttgcgctt cttaaaagaa ttgccgccgt tgcgataccg agcatacttc agcagagctt
      241 catttcggtc ggcaatatta taattcaggg cgttatcaac agcttcggtt cgggcgtaat
      301 tgccggatat tcggcttcga tcaagctcaa caatcttgtc ataacctcgt tcacgacctt
      361 cggcaacgga atttcaaatt acacggcgca aaacctcggc gcacgcaaat tcggccgaat
      421 aaaagagggc ttcaaggcag gcgtaaagct gacgtggatg atttgtattc cgattgttgc
      481 cgtttatctg cttgcaagcc gttatctaat ttatttcttc ctcgaaagcc cgacgggaac
      541 ggcaatggac acaggtatgc ttttcctgag gattctgact ccgttctatt tcttcgcagc
      601 cgcaaagctt gtttccgacg gcgtgctcag gggtgcggga cagatgaaag cctttatgac
      661 gggtacattc acagaccttg tactgcgtgt cgttcttgca ataattcttt caaaaaccgc
      721 cctcgcttca acaggaatct gggtctcatg gcctgtcggc tggtgcgttg ccggcgtgat
      781 ttctctcacc ttctgcttca agtatatgaa catggtgaaa tatgacggaa taatttaaga
      841 aaaagtgagg taacaattat gtttacaact gcttccggta gaaagattac ttattacgga
      901 catgaatatg caaacgagat agtaagaatc aagggcagca acaaaataat aaaatcaata
      961 gatgaattgt ttgacatact actgaaatca tggtgcaaag aaactgcatt cccgagttgt
     1021 caatccgatt atgactttaa aaatgacccg tcatacggtc agtgtgcaat tacagcagtt
     1081 attgtatatg atatgtttgg cggtacgatt cataaggtta aattttccaa cggaggaacg
     1141 cattatttta acaaaatcaa cggacactat attgatttga ccagtgatca gttcacactt
     1201 tacaatttgc ctgttgacta tgaacacaat gaagaagttc cacggcaata ttgcggcaaa
     1261 aatgccgaca cagctaaaag gcttaaactt ctgatttcac aaataggtca aaacctccat
     1321 aaataaacct gaaaggaaga ttctcatgtg cacagcggcg acatacaaaa caaatgattt
     1381 ttatttcggc agaaccttag actatgagtt ttcctacgga gatgaaatca cggtaactcc
     1441 gaggaactat ccttttaact tcaagcacac ggagccgatc gaaagccact atgcgataat
     1501 aggaacggcg cacgttgcag acagctatcc gctctattac gatgcagtca acgagaaggg
     1561 cctcggcatt gcggggctta attttgtcgg caatgcggtc tatgctcagc ctgttgatga
     1621 taaaacaaac atcgcacagt ttgagcttat tccgtggatt ctctctcaat gcgccacggt
     1681 tgatgaagtg cagacgattt tgagcacaaa tgcaaaggct caatgatttc tcgtacctct
     1741 caccgaagca gccgaagaac aatttttcgg acaagctttc gtttgatatg tacagcagag
     1801 gaatgggcgc tataggtctg ccgggggatc tctcctcggc atcaagattc gtaaaagtcg
     1861 catttacaaa aatgaattcc gtctcgggca actccgaaaa ggaaagcgtc agtcagtttt
     1921 tccatattct cggctcggtc gaacagcaaa ggggctgctg tgaggttgcc gacggcaaat
     1981 acgaaatcac gatttacacc tcctgctgca acgcaagcaa gggaatttat tactacacaa
     2041 cgtatgacaa ccgccggatt acggcggtcg atatgcaccg tgaggatctt tggagcaatg
     2101 aactgattcg ctatcccatg ctcacggaca gcagcattct ttggcaaaat taaaaatttc
     2161 ttaaaagggc ggccggcggt cgcctgtttt tatttctgtg atttgacatc acgtcctgcg
     2221 gtttaagtct tatttagaga tatcgcaccg tatcagcttg ataaatatag ctttcttcac
     2281 aaaactattt tacctcaatt tattttgaca atcatataat aaatgtggta gaataaatct
     2341 cggtgattta atgaaaatcg gaaatcttga aataaaagga tacgcttgtc ttgcgcccat
     2401 ggcaggagtt gccgacagag cctttcgcga gctttgcatg agctacggtg cggcatatgt
     2461 catcagcgaa atggtcagct ccaagggttt aacaatgcaa gacaaaaagt ctaaggagct
     2521 gctctttctc tcggatgccg aacgcccggc aggcgctcag atattcggtg acgatcctga
     2581 aatcatggca aatgccgctt tgaaggccat ggagttctct cccgacttta ttgatataaa
     2641 catgggctgc cctgcgccga agattgcagg aaacggcggt ggctcggctc ttctcaaaaa
     2701 tccggagctg atcggcaaaa ttgttaaaaa ggtcgttgag gtctcccccg ttcccgtcac
     2761 ggcaaaaata cgaatcggct gggataagaa cagcataaac gctgttgaaa ttgcaaaaat
     2821 aatcgaggct gccggtgcag acgctatcac ggttcacggc agaacaaagg nnnnnnnnnn
     2881 ggatcagatg tacgctcctc ccgtgtcgct tgacgaaatt gcaaacgtca aaaaagccgt
     2941 ctcgattcct gttatcggca acggcgatat tgttgacgga aagagtgcaa agctgatgct
     3001 tgacatgacg ggttgcgatt ttctgatggt cggcagaggg gcattgggaa atccgtggat
     3061 atttcaatgc ataaatgcat atcttaacaa agaagcagat ttcacagagc cgacactcga
     3121 agaaaaaatg gacgtcatgc tccgccatat cggcactctt tgcgaataca aaggcgtgcg
     3181 aatcggcatg cgcgaagcgc gcaaacacgc cgcttggt
//
LOCUS       DAWBSW010000216         3204 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4907_length_3204_cov_2.570975, whole genome shotgun sequence.
ACCESSION   DAWBSW010000216 DAWBSW010000000
VERSION     DAWBSW010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4907_length_3204_cov_2.570975"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          955 a          725 c          756 g          768 t
ORIGIN      
        1 aaacaaagcg cttcgttatc tgcgttcacg gccacaagtg caacggaccc gatgaattca
       61 gccacatgat gccgttctat cactacgacc tcggctataa ctaccttctc cccgacctca
      121 cgggtcacgg cagaagcgaa ggcgaataca taggcttcgg ctcttttgac gcaaagaata
      181 ttctcctttg ggttgattat ctgatcaatc gcttcggcga ggatatagaa ataattctcc
      241 acggcatttc aatgggagcg gcaaccgtgc tcaactgcaa tgagatgtcg ccgcccgatc
      301 aggtaaaaat tatcattgag gactgcggct tcacaagtgc cgaggctgta atgaacaaca
      361 ccctcaagga tatgatcggc ttcaagttca tgccgatagt aaaactcgga agccttgtca
      421 gcaaggttaa ggcgggctat ttcttctcgg agagcgatcc gctcaaaaat atgaataaag
      481 caaagaatcc cgttctcttc attcacggcg aagaggacac atatgtacct tttgaatacg
      541 gcaaggagct ttacgatgcg tgcccgacac cgaaggatta tctttgggtg cccaacaccg
      601 ttcacgcttt cagctattat aatgccaagg acgaatatga gcagaaggtc aaggacttta
      661 ttgcaaaata tatggataaa aaagaggaag ccgtgacagc ataataacat aaacaagggc
      721 acgcttgtgc cctttacttt tggaggaaac tatgaaatac aaaaatttat caaaagaaga
      781 actctcagga ataaagaaag agcttgaagc cgagtacgaa gcctttaagg cacagggact
      841 taagcttgat atgtcaagag gcaagccgaa caaggcacag ctcgatctga caaacgatat
      901 gctcgataca ataaattcag caagcgatat gaccgcttct gacggcatgg aaacaagaaa
      961 ctacggcgga cttatgggta tacccgagtg tcgtaagctc atggcggaaa ttgttgacgt
     1021 tgacgaaaag aacgtcatcc tcggcggcgt tgcaagtctt acgctcatgt atgattatat
     1081 ttcacagtgc atggtattcg gctgcggcgg caacgagcca tggatcaagc agggtgagat
     1141 taaattcctc tgcccctgcc ccggctacga cagacatttc ggaattcttg agcatttcgg
     1201 catcaagatg ataaatatta aaatgaacga cgacggcccc gacatggacg caatcgagga
     1261 atatatcaag gatgaaagtg ttaaaggcct tttctgcgtt cctaaatatt ccaatccgga
     1321 cggaataaca ttttccgatg aggtcgttcg acgcttcgct gctctcaaac ctgcggcaaa
     1381 ggatttcaga gttatctggg acaacgctta cattgttcat gacctcaacg acacaccgga
     1441 caagcttctc aacatcttcg atgaggcaaa aaagtgcggc aacgaggata tgtttattga
     1501 agtaatttca acctccaaga taagctttgc cggcggcgga atttccgctg tgatcgcttc
     1561 cgacgcaaac atagccgaaa taaagaagcg tatgagcatt caaataattt caaacgataa
     1621 aatcaaccaa ttgagacacg caagatactt caaaaatctt gacggcataa aggctcacat
     1681 gaaaaagcag gcggcaattc tcaagccgaa attcgacgcc gttaccgacg gctttgaaaa
     1741 ggcatttgac ggtacgggaa tcgcaagctg gaagaagcca aacggcggat actttatcag
     1801 tcttttcgtt atgaacggct gtgcaaagcg tgtaggtcag ctctgcaagg aagcgggact
     1861 caccctcacg accgtcggcg caacctatcc ctacggcatt gatcccgatg acagcaatat
     1921 cagaattgca ccgtcctatc ccgacgttga cgagcttaaa aaagccgttg aactgctttg
     1981 catatgcgtc aagcttgccg ctgcggaaaa attaacggaa gagtgattaa atgaacaagc
     2041 cctattatga agtaagagaa tttcataatt tcagagagct gctcaatcaa agtgagaagc
     2101 tttacggaaa cagtcccgca ttcaaaatta aaaaccaaat cggtcagatt cttgatataa
     2161 gctacacacg ttacaaggct gatgttgagg ctctcggcac ggcgttgctc aatctcggac
     2221 tcggcggcgc aagaatcgcg gttgccggaa tgaacagcta taagtggtgc acgagctatc
     2281 ttgcaatcgg ctgcggagtc ggcgttgttg taccgacaga caaggagctt ccgtttgacg
     2341 atattctcag catactgagt gtttccgaag cgaaggcgat catctttgat gaaaaattcg
     2401 gggaaaagct gcttgagcac cgtgaaaagc ttccgaaaaa tctgctgctt atctctatgg
     2461 agcagcagaa agatgcggac ggaatacttt cttatgattt gcttttgaac aagggctatc
     2521 atcttatcgg caagggcaat tcggattaca tcgaaagaga tgttgagggc aacaagctga
     2581 ccgttctcct ctttacctcg ggcacaacgg gtatgtcaaa ggcggtcatg ctcagcgctg
     2641 ataatatctg ctacgacgtc agagcaatta tgggctttgt taaaataaat caaggtgaac
     2701 gaattctttc aatccttccc ctgcaccaca cctatgaatg taccgttaca tttctttgct
     2761 gtatttacgg cggcgttaca atatgcttct gcgacgggct tagatatatt acaaaaaatc
     2821 ttgaggaata caagccgaat attttaattg ttgttccgct cgtgcttgaa aaattttata
     2881 agcgaattat gaaggcggtc gcaaaagagc gtggcggagc tgccaagata gctctcggca
     2941 gtgcgataac caaggttgcc ggagccgcaa agcttgacgt ttcaagcctt ttcttcggca
     3001 agataaaaaa agccttcggc ggctcgataa gacttattat ctccggtgcg gcaggaattg
     3061 accccgagat tataaagaac atgaaccgct tcggtataca gaccttccag ggctacggcc
     3121 tgacggaatg tgcgcctatc gttatttgca acagcgataa ggataacaaa tacgattcca
     3181 tcggtaagcc gattccatat gttg
//
LOCUS       DAWBSW010000217         3202 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4910_length_3202_cov_2.696536, whole genome shotgun sequence.
ACCESSION   DAWBSW010000217 DAWBSW010000000
VERSION     DAWBSW010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4910_length_3202_cov_2.696536"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    554..563
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          963 a          684 c          707 g          838 t
ORIGIN      
        1 ataatggtta gtgtgccata ttgtaataat tgttaggagc ttttttatga aggagatcga
       61 gacatcgtcc ttgaaaaata taattgacac ttttccgttc agcggcgagc cggtaagcat
      121 caagtcaacc gataccggtc ttattaactc gacctatatg ctcacctttt cagacggaaa
      181 tcaggacttt caatatgttc tgcaaaaaat aaatacgaat gtattcaaaa agcctgacga
      241 gcttatgtca aatattatga atgtaacggg ctttctccgc aataagataa atctttcggg
      301 cggaaatgcg gcacgggaaa ctctgacgtt tctttataca aaagaaaata cgccctatta
      361 ccgcgatgaa aacggcggct gctggagaag ctataagtat attggaaaat gctatacatg
      421 cgacaaaatc gattccgaaa tcaaggcttt ccgttccggc aaggctttcg gtaaatttca
      481 aaatatgctc tcggactacc ctgctgcgaa tctttttgag actatccccc atttccatga
      541 cactccttca agannnnnnn nnnttcaaga tatgaggcac tgaaaaaggc tgttaaagat
      601 aacctttccg gcagacttca aagcgttaaa aacgagcttt catttgctct tgaccgtgaa
      661 atggacgctt caaagcttgt tgaccttgcc gctgaggaca gaataccgat gagagtaact
      721 cataacgaca caaaaataaa caacgtcctt tttgacagca tcacaaacga ggctatttgc
      781 gttatcgacc tcgacacaat catgcccggt ctttctctct atgactttgg cgacagtatc
      841 cgctccggcg ccgtaacatc ggacgagaac gagcaggatc tcgataaata cggtcttgat
      901 attgctcttt tcaagagcta tacagagggt ttcctttccg aagccggaca tgctctcaac
      961 gatgaggaga ttgcaaacct tgccttctct gccaagctta tgacccttga atgcggagtg
     1021 agattcctaa ccgattatct caacggagat acatatttca aaaccgatta tcccgagcat
     1081 aatcttgtca gatgcaggac acagttcaag ctcgttgagg acatagagaa gcatttggac
     1141 gaaatgaacg cttgcgttga tgagatatac aggaggagtt tgattgaagg ctgagaaaaa
     1201 aggaattgtc attatttcgg cggtcatagc gatagccgtt gtcacgacaa taatccttat
     1261 tcctctttta aggcacaatt ccgtattggg atatatgaaa gaggatataa aaaatgttga
     1321 taacatttcc ctcatctccc gatgcagtga gatcggcggt aagaaaaact ccgttgccgg
     1381 cgttaaggaa agcgtaagac tcggtgcaaa tgcggttatt gttgaccttt gctttcgcaa
     1441 ggacggaacg cccgtaatca ccgataacta ttcaaatgcc gacagtgcag aaaccgttga
     1501 ggagcttttc aaggcgatga gcgaggataa attcaggaaa atcgggattt atctcaacat
     1561 tgttcagctt tcggacattt caaagcttaa tacccttgcc gtgagctata atatgcttga
     1621 caggctgttt ctcatcggca ttgattccga ccgatatgat ctcataagct cggacgatac
     1681 aataattccc attttgcttg actacacatt cggctccgat gagcttgcgt cagttaaaag
     1741 cggcaaattc aaaagacccg atattcttga agaaaagggt gcgttgggac ttgttctgga
     1801 caattcgcaa attacccccg agctcgtcga cggagttgac ggtactgcgg aaatgtgcaa
     1861 aaccatactt aacggaacgg gaaacatcat tgttaccgat atcaaagaag cgagagaaac
     1921 gcttgacaaa tggactgaaa aaatacagga acgctacaaa aagtccgtgg ataaatcaat
     1981 aaaagagctt tcaaaagaag cagaataata ttaaactgaa acaggagtgg taaacatggc
     2041 aaaaattctt attgcagacg acgaaagcaa gcttcgcaga ctggtctgtg attttctcag
     2101 acgttcgggt cacacccccg ttgaggcaag caccggtcgt gaggtcctcg atattttcaa
     2161 ttctgatgaa aaaatcgatc tttatcttct tgatatcatg attcctgagg ttgacggatg
     2221 ggaaatctgc cgtcagattc gcaagacctc gtctgttccc attatcctcc tcacggcaag
     2281 aagtgccgaa tacgatcaga ttatgggctt tgacgcaggc gccgacgact atgttacaaa
     2341 gccgttcagt cttgcggttc tcatgaaaag aattgacgtt cttctcaagc gttcggcttc
     2401 gggtaaggta agaacggata aggacataat ctcccttgac ggcttgctca tcaacaatga
     2461 cgctcatcag gttacaattg acggtaagcc tgtcgagctt acgctgaagg aatacaatat
     2521 tcttgagaag cttatttcaa gccgaggaag agtatataca agagaacagc tcctcgatga
     2581 tatctggggc tatgattatg tcggcgacac aagaaacgtt gattcccatg ttgcacgttt
     2641 gcgcggcaag ctcggcgtat ggggctcaaa tcatcttaaa acaatttacg gcataggtta
     2701 taaaattgag gtgaactgat ttgagtcctc cgaagaatgc agaggacaag ccgcttgtcc
     2761 gcaacatgaa agcacaggtg tttcttcaat ttgtgctcat attcatggtt attctctgcg
     2821 ttatcctgct gacttcaacc tcgctttata ccaacatcat cacaggaatg acgctttcaa
     2881 atctccgtga tgcggtcgat gaggttaagc ttattgatgt tacaaggacc gacaatgtca
     2941 cccacagcgt tgaggaaatc gagcgaaagc tttctgtttt cattgaggtc tacagcaaag
     3001 acccaggtga caaggatgac gaatactctc ggattattta ctctaaattc gcctatgaca
     3061 gtttctatga ccgtgaagga aaaagatcct acagtcaaag caccacttca tttgtcggtt
     3121 tcagagacgg aatttttgaa atcgacagaa aatacgggga cggaagctat gtcggaaagc
     3181 tgaaaaatcc caacaacaca ta
//
LOCUS       DAWBSW010000218         3200 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4912_length_3200_cov_2.317011, whole genome shotgun sequence.
ACCESSION   DAWBSW010000218 DAWBSW010000000
VERSION     DAWBSW010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4912_length_3200_cov_2.317011"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          842 a          680 c          684 g          994 t
ORIGIN      
        1 ttctcggaat gagcagaacg ttattttcta aatatgagaa cggaattgca aatccgccgc
       61 ttcaggttat gagaaagctt gcaagcattt ataacgtcgg attggaatat cttatatttg
      121 atgacaatac ttcaatcaag ctcaatgacc cgaatgaaga ggacgttaat tcgcccaatg
      181 cccctgtttc aagaataacc gatctcagac cgattgagaa ggagatcgta ggcaaattca
      241 gaactttttc cgaaaaggat aaggaagaat ttatcaaagt atttttttct gataataaat
      301 aagactgaaa aacacttcag cgtaaaaaag gacagaaacc gatttgcatt ggcttctgtc
      361 ctattatttt tagcttattt taccgacaag caagacgtag cacgctctta ttgttcgtga
      421 gcctgccaac ccgtcaacct cgagcttagc cgctctttgt acctgctttg tcgcttcaac
      481 ggtgccgtca ccgaaaatat tgttatcgtc aacgccctgt gtgatgattt ttgctttttt
      541 aagagatatc aaaagctgtt tgtaagcgta aacgccttca ttgcgatcac ccttttgaaa
      601 ataacctctt gtcatatcgt ttcctccgat gttctgtgat tttggtattt cttcatcgtc
      661 ttttttgccg tgaatcagtg acggataatc aacatatccg tagttgaggt cggttttgcc
      721 gtcgattccg ttcaccttgc ccgacgaggt gtactgccat actccgtatg attttttgta
      781 ggtgttctta tcgttgtact gagcgaccca cttggctttt ctgtcatacc acgaatctgt
      841 cagacgtgtg ttgaaccagt ttgtgttggc ataaattcct gcccagtagc cggctttttc
      901 aattccgtta acaaaaactt ttgccatttt gagaattgtt gaatttgact gactgcccgt
      961 agttgcttcg tcctcaaggt catagtagac agggtaggac ggctttttgc ttttgagcag
     1021 tttaaggcag ttatcaagct ctcttttcgc ttcttcaaca gattcggcat agctgaaata
     1081 gtatgcgccc catgggattc cgacccgctc acattcctta acatttcgct cgaaatacga
     1141 atctacacct ccgttgccgt agccgcagcg aatgattgcg aatttaattc ccgaagcttt
     1201 taccttgttc cagtcaattt ccttctgcca atatgacaca tcaacgcctt tagttgccat
     1261 cggtttcatc tcctctaagc attttgtccg ctttttgcgc attgcccgta aagctgttgt
     1321 ttttccacca tgaccaaacg gagccgataa ccgtcaggat atacgttgcg tattcctgcc
     1381 attgatcgac gtcgatcgct tcgcttctct tgccgaggat aacgcaaagc tgacttatga
     1441 gtgcaagtaa gagaactgct gttcttgccc atgtctgtac ggaaacacct ttgatattta
     1501 tttttttcat gttttaccca cctttctgtt tcggtacaag catattttat aaaaaataca
     1561 atattaagcc gaattttcgc ccttttatga tatttgggga taaatgccgc cttttttcga
     1621 taggacggcg ttatataata tgcctgcaaa aaggtgaata tgacaatttt tatatgtaat
     1681 tattctaaaa gtatacggtt gctcttttgc tattgacccg tttttgccgg tatgatataa
     1741 tttttatatc aatttacgaa aggtgagaga aatgcgctac ggaatgtgta tgggcatcga
     1801 cagccctcaa aacgttaaga cagccaagga tgcaggcttt gattatatgg aatgcggttt
     1861 cagcctgctt tcgagagccg atgaagaaat gtttgaaaaa ttcaaggcgg ctcttgagga
     1921 aagcaatata aaatgtgaag cggcgaattg ctttttaccg ggcgatcttc ctgttatcaa
     1981 tcacgattac agaagcagga aatacgttga ttatattgaa aaaggcatga cgagaggtaa
     2041 gcagataggt cttgaagtta tcgttttcgg ctcaagtgcg gcaagaagcg ttcccgaggg
     2101 aacaagcttt gccgagggct tccgtcagct cggtgatttc ctcaaaagcg ttgtttctcc
     2161 tcttgccgag aaatacggca tgacagttgt tatcgagccg ctgagaaaaa atgagacaaa
     2221 catgattaac accgttaaag agggcactat gctcgctgtt ctttccgaaa acggtaatat
     2281 cgcctgtctt gccgatttat atcacatggt cgatgatagc aacgacgata tccgtcagct
     2341 caaaggctca atcaagcatg cgcacttttc aaatccttcg tcaacgaacg gattaaagag
     2401 gggattcccg aaaagctttg atgagtacga ttatcaagca tttattgatg ctctgaaata
     2461 tgcaggctgt gagcgctgct cggttgaggc taagtgtatt gattattcaa ccgaagcttt
     2521 tgaagcggga aatcttatta aacagctcaa ataaagtcaa aaaaaaaaga aggtcggaat
     2581 tgctccgacc gtttcttttt gcttaaaact tattgatttg tccgactgcg tactgaagaa
     2641 ttttcagcgc atcggcggaa ttaacccttt tgtcattatt gacatccgca agtgtaattt
     2701 gctctgaact gagctttgca ctgccgaccg aatactgaag aacaagcaat gcgtcggcgg
     2761 aatttattct tccgtccttg ttgacgtcac ccttttgtga ggttgagttg accttgaatt
     2821 tgagcgttgt gttgcaggta aagtgctgat cgtcggaata gggaagaacc gttcccgtca
     2881 gatccgaggt agtgcaaatt gcactgtaga aataacctgt tttgtaattt tttgttctcg
     2941 ccctctgcgc tgggcttagc cgattttgaa acatcaaagg ttattgttat aagatcctcg
     3001 ttgaggttac cgacagccgt agttgtaatc attacgttcg gtgtcagcgt cactctcaaa
     3061 aactgatgat cctttttgta ttcggcaagc ttttctgcgc tgtaattagg ccaaccgagc
     3121 ttcctgtttc cggcagagat cttttcccaa tcaacgaagc tcgcatgagt cttgcacgaa
     3181 acgccgtaaa gacggcactt
//
LOCUS       DAWBSW010000219         3196 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4918_length_3196_cov_3.054760, whole genome shotgun sequence.
ACCESSION   DAWBSW010000219 DAWBSW010000000
VERSION     DAWBSW010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4918_length_3196_cov_3.054760"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          824 a          752 c          656 g          964 t
ORIGIN      
        1 ttgccattgc ctgctcccga tcaacaactg tctgctcctt tgcgcaaagc ccgttgacct
       61 ttatcttttt ttcaagaaaa atcaaagatg tgtcgccgtt gtagcatcga aggcggaact
      121 tttcacgtcg gtcaacgccg ttcacctttt caagcaaagc cttgtcgtca agattatcaa
      181 aataaagact gcgaatcttg tatcttccgt tgattgcgtg agggtcctgc tgcataacag
      241 ccgacagcct cgacctcaaa gcgagcatat cgcaatagcc tatctcgtgc ttaaattcgt
      301 gtctgaagtt catgtttatc actcccttgc tgtttttgtt gtctatagca taacgggctt
      361 accttagcta aagtttagag aataaatttt ttgcaaaaaa aattgcccac aaaaaatgag
      421 caaaataaaa agcagataac cgaagttacc tgcttcattt gttttgattt ttaatcaata
      481 cttgaacatg aagaaaatcg taatgccaag tgtaagaata aagaaaacga ttgcagaaat
      541 tcttgtaagc ttaacgagct tctgttccat agttctgccc ttactcttgc cgaaaaatgt
      601 gtcggcaccg ccggaaatag ctcccgaaag gcctgccgaa cggctctcct gaagaagaac
      661 aacaattata aggaaaatcg caagaattat aagaactgct ccgaatacat actgcaaagc
      721 actcataatc aaacctccat aagtattttt accgatttat tttagcacac actttcacaa
      781 aatgcaatag caattaccat aattttttca gaaagcaaag tatttcaatg aattattgca
      841 aaaaatcggt tcattataat atcaggcggc aaaaacaagc cgccgtcagc ggaaattata
      901 aataaccggc gtttgcagtt ttccgctgtt cgccgttcac tttttaaagt gagcggcgat
      961 ttttttcgaa caatatcgca caaattgaga tgtgcgaatt gcgtagcaaa atttttcgtc
     1021 ggtttgtgcg gtgttgcctt tatatcagtg aaagctgttc accggcgtcc tctgcgctca
     1081 gggttgctcc cgaggcagga agatttttcg tcttgtatct gtagggcttt gcaaattcgc
     1141 ccttgcgata atccctgacg aatttaacaa catgaccctt tttcagcgtc atgaccgcaa
     1201 cgccctgagt gtccttggtt gtctttggaa ctattgcccc tgtgttgaac agcagcagct
     1261 tgcctgccgt tgattcaatc acaagatcgc agtcctcttc aatctttctt attgccgcta
     1321 tgggtgactt gttactgtat gccttgataa gcttctttct gttcgttttg gtctgatatg
     1381 acgcaaggtc aacctttgca acctttccgt tttcaaagaa gaacattaca tatcccttgt
     1441 agtcgagcgt tgaaaccatg taaattgcgt tttctccgct gtccatctgg agtttatttg
     1501 caacgaaatc gccgagctgg ctcgctttgc cgaggtcaaa atcggcagcc tttgctttat
     1561 atacctgaca ctgatccgta aagaacagca aatccgtatt attagtcgct tcgatttcaa
     1621 gcagcatttc gtcgccctct ttgagcttct gctcgccgct cattctgagc gcctgcggcg
     1681 ttatcttctt gaaatagccc tcctttgaga agaaaagatt tacaggaaaa tcgggaattt
     1741 cctcctcggt ctcctcctcg gctacttctg aagcgaatat gatccgggtt tttctgtcct
     1801 gaccgtattt atttacaaca tcggtcagct cattgacaag aatcttcttt atcttcgact
     1861 tatgagctaa gatatcctcc attttttcaa tgtcggctct gagctgttcg atatcctgcg
     1921 ttctcttgag aatatactca cggttgaggt gacgaagctt tatctcggca acataattcg
     1981 cctgaatttc atcaataccg aagccaatca tcaagttggg aacaacttcg ctttcttgct
     2041 cggtcgagcg gattatttta atcgccttgt cgatatcaag aagaatcttt tgcaaacctt
     2101 caagcaaatg aagcttgtct ttagccttgc tcaaatcaaa ataaactctg cgcttgacac
     2161 attctgttct gaatgctatc cactcattga gtatctcgcc gactcccatg accctcggcg
     2221 cacccgcaat cagtacatta aagttgcagg agaatgtatc ctcgagagga gtagccttat
     2281 aaagcttctg catgagcttg tcgggatccg tgcctttttt gaggtcgatt gtaattttaa
     2341 gtcccgactt gtccgtttcg tcacggatat cgctgatttc acgcagagat ccggacttaa
     2401 atttttcgat aaccttgtcg ataatcgctt cggctgttgt tgtcggcgga atttcggtga
     2461 tatcaatgca ctttccgtct ttatcgtaat tatactttgc tcttatcttg aagccgccgc
     2521 gacctgtttt gtaaatcgtg tcaataacat ctctgtcata gagaagaaga ccgccgccgg
     2581 cgaaatccgg agccttaagc gtttcggaaa tatcggcttc gggatcttta atgagcgcaa
     2641 tcgtcgtttc acaaagctca cgaagattga acgggcaaat tgagcttgcc atacctacgg
     2701 caatacctgt cgtcgggttg gcgagaatac tcggaaagcg cacaggcaaa aggctcggct
     2761 ctttcattgt gttgtcgtag ttatcgacaa aatccaccgt atccttatca atttcggcaa
     2821 aaagctccgt acagatcggc gcgagctttg cctctgtata tctcggagcc gcccactgca
     2881 tatctcttga atacgccttg ccgaagttac cctttgagtc aacaaacggg tgcaaaagcg
     2941 cttcgtagcc tctcgaaagg cgaaccatcg tatcgtaaat cgcagcgtcg ccgtggggat
     3001 tgagcttcat agtttgaccg acgatgttcg ccgattttgt ccttgctccg ttgataagcc
     3061 ccattttata cattgtataa aggagcttcc tgtgagaggg cttgaaaccg tcaatctcgg
     3121 gaattgcacg tgaaagaata acgctcattg catatggcat atagttcgtt tcaagcgtat
     3181 cggtgatatt ctgacg
//
LOCUS       DAWBSW010000220         3173 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4969_length_3173_cov_1.896408, whole genome shotgun sequence.
ACCESSION   DAWBSW010000220 DAWBSW010000000
VERSION     DAWBSW010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4969_length_3173_cov_1.896408"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1599..1608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          746 a          695 c          727 g          995 t
ORIGIN      
        1 gtcatattct gagcgggcat ggtctcgggc acttcaggcg accatcccat gaaatcatag
       61 ccgacttttt tcggagactc aggtggtacg atatcaaatc cttctttgat ttttagctcg
      121 gtaatgtgtt catcaatgat ccacttaatg gtgtattcac gagcgacaac gtgtacttta
      181 cagcttgcgg ttattgtctc tccgtcggta ttgcttatgc ttgcagtgac tgttgctgtt
      241 ccctcgccga ctgcacctat aacacccgat tcgcttacta aaacgacctg ctcgtccgat
      301 gactcccatg tgatcatcat gggtgttgcc tcttgcggga gtatggatat acttaacagt
      361 tttgcactgt tatctccggt gagatccagc gttacttcgg aatcatcaaa tgacatctcg
      421 gaggcctcga cggttacatt ggtgaatttt gcgacaagat tggtattctc ggtaacaacg
      481 aagccgtaat tcgcatcggc cgaaacaagc tctccgcttg caaaccaacc gaggaagctg
      541 ttgtttgagt cggtagaagc attaagggta atgtagtcac ctttagatac ggattcgtat
      601 ccgtttgcgt agccgacacc ttcaattgta acattcacct ttatatcgtg cagaagatct
      661 tcttcaagaa tttcggtttt ttcaatcgtt tcgcccaatt ctgttacaag agccaaatcg
      721 ttgatttcga aatcttctcc cgaatattcg cagcacatct caaagccttt ttctacagtc
      781 acatttgcac aatttcattg agatcgttat catagatttc aatatcgatc gggcaattga
      841 cagtgcccaa aaagcctttt ctgtagcgtc cttttgcata agtataggct tgatcgtagc
      901 tcattgttgt gacataagag gcataggttt ccgctgtatg ggcatattca aagcggcgag
      961 ctaactttcc ttcatgaata aaaaatgctg ttaatgccat atacaatttg tcgccggatg
     1021 taagtgctag ttgagaaatt gctgtagcca tatcaaacat tgacgctgtt ttactaacaa
     1081 aatggcagat tgtattttga aagtatcggt ataacgaata ccaaatcggc gacgtgcttc
     1141 gcaaaaagcc acctatataa aagctgttta gacctccaaa tctgcttgtc atggcaaata
     1201 ttacgctaaa cacttcaaac tcgccggttc cgtatgggac ataattgctg ccatagtact
     1261 ttctaaactc agaactcatt ttgtttttat agatattata gctgtagccg ctctcgtttg
     1321 ttttgcttgg cagagtaagg gtaacgccgt atctgccgta gccccatgcg ccgggcagca
     1381 cttttgtgac aaaatcttcc ggattcacaa aattgaatat gttggtatat atggtatttg
     1441 ctctggtgtt ggattttgtc gtgtttggcg atgcaaaagt ataagtaaat attttactgc
     1501 ttgtagggta aatgcttgag cctgatttgg atgaatctat gagctttgcc gccaagacgt
     1561 tcgcaacggc agcgcccctg ctgtgaccgg taataatgnn nnnnnnnngt aataatgaat
     1621 tcgatgtttt tgcctccggt aagattgctc cgcctcaggt agctgtcaag attgttctta
     1681 acgaactgtg ccgcttgctc gaaataataa tgaccgctgc cggtgtagtt ctttttgtct
     1741 aatttcccgc gagggtcaaa atttgaatac cattcgttgc ctgtcgttcc tctgagagct
     1801 accaaaacaa cggtgtgctt ttcgccgtta cgcacatatt cttttctcgt tataataaag
     1861 ctgttttttg catacacgga gttgcttttg cttggccaat aaattgctgc gtttccgttg
     1921 gaatagccgt tttttagttg tgcagcgctc gggtcttcat caaaggtttt gaagcccaaa
     1981 ttgttcaatc cattgcgcaa gttttcaacc gatgaatatg tcagcagaga tatctgcgaa
     2041 cagaatttgg cgaggttgtg attatagctg tatgaatccg tgtttaacca gttcgcatcg
     2101 aacgaaacat tcattttgcg attggcatca cccaagacca attgacccgt acttttaata
     2161 ttgctgttat acggcttgac ggtaacggtg cattttgcag tctttccgtt gtcgtttgtt
     2221 ttgaccgtta ttgttgccgt accctctgtg cgcgccgtga tcctcccgct tgagtcaacg
     2281 gtcgcaacat ttgtgtttga ggattgccat gttatgccct tgtttgctgc gtttgatggc
     2341 tttacggcgg cagtcagatt ctccgacttt cctgcaaaga gcgtgatttg agacctgttt
     2401 aggctgactc ccgttacttt tatgggctca tttttgacag taacggtgca tttggcggtt
     2461 ttgctgccgt cctttgtcgt tacggttata gttgccgtgc ctgcggcttt cgcagagacc
     2521 ttgccggagg agctgaccgt tgcgatattg ctgttgcttg agctccaggt aacatttctg
     2581 ttgcttgcgc cggacggttg gatcgaggcg gagagctgat atgtttttcc tttgatgatc
     2641 gaaatgcttg actgatttaa tgatatgccc gtgacaggct gagagacaat caccgtgcag
     2701 gttgccgtat agccgccgtc gccagtcctg gcggttatta tcgccgtccc ttttgatttt
     2761 gcagttacca gtccggcgct gacggaggca accgccggat tagaggactc ccaatgaact
     2821 gttttgttct gtgcattttc gggctttacg gaagcgttaa gctgctccga gctgcctata
     2881 gaaacggaga ttgaagatct gtcaagactg ataccggaga cctttacgtt gccggaactc
     2941 tgaaaatgaa tcgttgcatt tttcagacag ttgttgccaa acataatatt aatggcgttc
     3001 cactgctgtt ctgtgccgga ataaaagaca tctttcaagg cagtacaatt tataaacgca
     3061 taaataccta tgctaataat actgttagga agtgcaatgc ttgcgaggcc gctacaattg
     3121 gaaaacgcac accagccgat gcatgtaacg ccgtcgggga tggtgatgct tgt
//
LOCUS       DAWBSW010000221         3163 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_4991_length_3163_cov_2.560167, whole genome shotgun sequence.
ACCESSION   DAWBSW010000221 DAWBSW010000000
VERSION     DAWBSW010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4991_length_3163_cov_2.560167"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1225..1234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          953 a          678 c          655 g          867 t
ORIGIN      
        1 atgcaaatgt aaaaggaatg aaaaagggaa ctatggcatc ttcttatagc attgttgttc
       61 tttacgaaaa agatgaaaag gaatatttga taacttgttt agctgcgttg tcaaatgagg
      121 gaagatataa agcggtgcag tccgctataa atacgattat taaataatag tgaagtatcc
      181 tttaaatgaa acattaaaga aatcccccct acgcacaagg gacagccgag aaaatcgact
      241 gtcctttttc tatgccgaca ggctgcccga atagcagaaa ctccggctca tatcaagggt
      301 aaaatgaacg gctgctaagg cagccgccct tgacatgccc cgaattttcc gctactatgc
      361 aatcaggacg tgtaagcccc tccacggctc acacgcctcg ctattacttc gctttttata
      421 ccattctggt gtgtttacac aaaatttgaa gaagagcctc gacccctgcg aattgagctc
      481 ttatcgtatc tctatgctgt gttgattttc gtacttattg tagggtgcga tgccaatatc
      541 gtacctttga actattcttt acatcggact tgaaagccac agataacaga ggacggctgt
      601 tccgaaaatt atgaatgccg ttatgccgac aaacaatgcc cactcgcggc atttaattcc
      661 taaaatcaat gccgagatgt ccgttaaaaa caaaaataaa tatataatta tgagtataca
      721 tgtgaatgac attatgtttt cgccctcagt tcaaatgctg tttttcgctc ggaatcgaaa
      781 taaaagagcg tatagcttct gaaattatca acgttgaatg tgtaaaaata atcgtccgtg
      841 tcaatcatgc ttttgttgaa ggtatagacc tttttaatgt cgtcaccgag cagaaaatcg
      901 tcgtatgtta cctcaatgcc gctctcgaat ttgtcaatat atttgtttat ttcttcaata
      961 tctttcggag tgacattttt gaagtattcc gatttatcaa actcggcagg ggagaggttg
     1021 tcgtcaaaat aatattcaat atattcataa ccgtccattg caccgaaaaa atatacctca
     1081 tggtcgttat attccggcat ggatttttcg acatggcttt gaactgtgtc gggttctcct
     1141 tcaataagtg atgaaacggt aaccgttccg acaaacccta agataagaaa tccgattatc
     1201 gaaaaaaagc ctactgttac ggcgnnnnnn nnnngtcgag agcttcgcag attttgtcaa
     1261 gggtctggat tttaattgct ttcgcttttc cgtttttgag aatcgaaatg tttgccatag
     1321 ttattcccac acgttccgaa agctcggtaa cgctcatttt tctcttggca agcataacgt
     1381 cgatgttaac tataatcatg ctttcacctc atatcgtaag ctcgttttct tcctgcaatt
     1441 cggcggcgtt gcgaatgagc tgtgaaagcc ccatcgccgc aataaaaata gctatgccga
     1501 taagaacaat tatgcttgag aatagaagaa cggacgggtg gctgatgtca agaagaagat
     1561 aaattatgtt gcctgcgagc acaagagctg cgtcgcaaat agagcttatt gcaatgtttt
     1621 tgagattttt tgaattgtcg agacaaaagg gcttgttcct gccgatattt gacgccgttt
     1681 tccatgaata gataagcgcc ggaatcatag gaatcgccgt tgaccagaga aaagccatcc
     1741 aaggcgctct gagcttgtcg atattatccg catcggaaat gatgagcatg agtgaaatta
     1801 cgctctgaat cgcaaaggcg caaagcccca tagcgatgat aataaattta agactgcccg
     1861 caatcgtttt gagagaaatt ttctttgaca taatttacct ccgaaagttt tgttgtctgc
     1921 attatagcat tagtatatac gaatgtcaat agaaatttat cgtaaaacaa taaatatttt
     1981 ttgaaacacg gaggatgcaa ccgacgacaa tgttctataa agttgtatcc ggcagaggtc
     2041 gatgaccaca tcaaactcta cgaaatgtgc atttatttta acaaatcgta gtgtgcgatg
     2101 cccacatcgc accaaaaaga tttcagctta atctgttatt ttttctttat cctcccatgc
     2161 aggggaggca agagaataca acaaaaaaag agccgagcac cgttgcgatg catcgactct
     2221 tatattttat taaatcaata tgtgtttgaa aacggtgtga aaagcttgcc gtacttgaat
     2281 tcaacgggtt cacggaactg cgacgaacgg taaacactca gcaccatgcc tatcgcaatg
     2341 taaatacaca ggttggacga accgcctgcg ctcataaacg gcagtgtaat tccgacaacg
     2401 gggagaagct tgagcaccat gccgacgttg acgacaacct gagttattat cataaccgca
     2461 acggcactgc aaatcagctt tcccgtgaca gaaattgctt ttttgcctac gacaagaatt
     2521 ttaatcgcaa ttgcaagcag aagtcagaga acgacaatac atccgacaaa tccgagttcc
     2581 tcgccgacaa ccgtgaaaat catgtcgttt tcactctcgg gaacaagtcc gttctgcgtg
     2641 tatgttccgt tgaaaagtcc tgtgccgaat acgccgcccg agcttatggc gttcattcct
     2701 cgttcctgct ggaagatgac ttcgttatat gttgcttcgg tcaggttgct cggagcgtat
     2761 attgcagtaa atctctgctt ctggaagtcc gaaagaacct cgaaccatgc aatcggagcg
     2821 agcaaagcaa cggcgcctgc tccgaaaagg aaataacgca gctgcaaacc tgccatgaaa
     2881 agcattccga cagtaataaa catgaaaaca agcgcagaac ccatatcacc ggtgagaaca
     2941 acaagtccta tggcgatcat tgcgtgaatt ccgagcagga taacattctt gagcttattt
     3001 atctcgctgc cgacgctttc aagatgaacc gaaaatgtga ttataaatcc aattttcaaa
     3061 agctctgacg gctgaaaata gaagcttccg agctttatcc acgaaatagc gtcgtcacga
     3121 ccctccgggg agcttcctat tacgaaaagc gaaagcataa gac
//
LOCUS       DAWBSW010000222         3138 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5042_length_3138_cov_3.115148, whole genome shotgun sequence.
ACCESSION   DAWBSW010000222 DAWBSW010000000
VERSION     DAWBSW010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5042_length_3138_cov_3.115148"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          783 a          790 c          729 g          836 t
ORIGIN      
        1 atgttcatcg gcggaaaata tattgataag tcatggggag caaacgacgg aattgttccg
       61 cttaaaagtg cactatatcc ttttgacgag gatcacatta catatgatga aacaagaagc
      121 ataattcccg gtgtttggta tgttatgccg acaatttacg gtgccgacca ctatgatttc
      181 tgtaatgccg cagacgaaaa ggcgttcggc tcacgtcaag gcttctttga tttctacaca
      241 gatctctcag agcttatttg caatatctga tttttaaaat tcataagtct gcaccccgat
      301 tacaattgta gtcggggtgt tttgcaatat tatattgatt ttgagagaac tgaaaaaagc
      361 agcaaggatg gtttctcatc tctgctgcaa tttttaatta aatcagttgt tgtaaagtcc
      421 gcgcttaacg taagttgtgt ggagaacctc atgcgcctta tggctgccgg gctcgccgaa
      481 gaactcgtca tatacacgct tgatagccga attctcatgt gacttacgat tttcgttgtt
      541 cttgtcaaga ttgtaaagaa cggaagcacg cttgcttctg aagtcctcaa agttgcgaac
      601 cgctgcggaa acgatcggct gtccgccgcc gttgatacat ccgcccgggc aacccatgat
      661 ctcaatgaac tgatactcgc ttgtaccgtt cttgatctgc tccatgagcg tctttgcgtt
      721 ctttgtgccg cttgcaacgg ctaccttgat actgagatcg ccgactgtgt atgtggcttc
      781 cttgacgccc tccatgcctc tgacctcggt gaaatcaaca ttatcaagag cctgacccgt
      841 aatcttctca actgcggtac gcagagccgc ctccataacg ccgcctgttg cgccgaaaat
      901 aacggctgcg cccgagccct cgccgagagg actgtcatag gtctcatcag ggagtgaatt
      961 aaagctgatt cctgcgctct taatcattct tgcaagctca cgggttgtaa tagagatatc
     1021 gacatcggca tagccggatg cgctctgatc ttctctgcca acctcgaact tcttggcggt
     1081 acagggcatt acgctgacgc tgacgatctt agcaggatca atatccatct tctgagcgta
     1141 ccatgtcttg agcgttgcgc cgaacatctg ctgaggtgac ttgcaggttg aaaggtgatc
     1201 aagctgttcg ggatagtaat gctcgcaata cttgacccag cccggtgaac aggaggtgat
     1261 catcgggagt gcgccgccgt ttgtaattct ctcaataagc tcgtttgact cttccataat
     1321 ggtgaggtca gcggagaaat cggtatcaaa caccttgtca aagccaaggc ggcggagagc
     1381 tgcaaccatc ttgccctcaa cgtccgttcc gatcgggaga ccgaactcct cgccgagagc
     1441 cgctcttaca gccggagctg tctgaacaac aacgtacttc tcgggatcgt tgatagcctc
     1501 aagcaccttc ggtgtgtcat ccttctcacg gagtgcaccg gtcggacagt ttacgataca
     1561 ctgaccgcag gcaatacatg agaactcctt gatttctctc tcaaacggag atgtgatgca
     1621 ggtatcaaag ccacgggcat ttgctcctat aacggagatg ctctgctcat cgcaggctgc
     1681 aacgcagcgg cggcaaagga tacatttgct gttgtcacga atcatgtgag gcattgaatc
     1741 gtcataagca tagtcgggcg taacgccgtc aaacttgctc tcgttctcaa cgccgtattc
     1801 cttacagagc ttctgaagct cgcagtttgt gcttcttacg caagaaagac acttgcgctc
     1861 atgagtcgaa agaataagct caagagttgt tcttcttgaa ttacgaactc tgtcggaatt
     1921 ggtgaggatc tccattccct cgtttacggg gaaaacacag gaggcaacaa ggcttcttgc
     1981 tcccttgacc tcaaccatac agatacggca tgcaccgatc tgatttattt tcttaaggta
     2041 gcaaagtgtc gggatttcga ttcctgcata gcgggcagct tcaagaatcg taattccgct
     2101 cggcacactc agaggcatgc cattgatttt gatattaacc atttccattt ctgacatcct
     2161 ccttatttct taataattgc gccgaagcgg catgtatcca tacatacgcc gcactttacg
     2221 cacttgcttg tgtcgatagt atgtgctgtc ttaactgcac ctgatattgc accgaccggg
     2281 cactttcttg cacaggcggt acagccctta cacttatcag cctcaattga gtaggaaaca
     2341 agcttcttac aaacgcctgc cgggcacttc ttgtcaacaa tatgagcgat atactcatcc
     2401 ttgaagaaac gaagtgttgc aagaacaggg ttcggagctg tctgaccgag accgcaaagt
     2461 gagttgtctt taatataatg agcaagagat tcaagctcgt caagatcctc gagagtagcc
     2521 ttaccgtcgg taatcttctc aagaagctct ctcattctct ttgtaccgat acggcacgga
     2581 gtacatttac cgcagctctc atcaactgtg aaatcaagga agaacttagc gatatcaacc
     2641 atacagttgt cctcgtccat aacgataagt ccgcctgagc ccatcataca accgatagcg
     2701 gtaaggttat catagtcaat cggtgtgtcc ataagggaag caggaataca tccgccggac
     2761 gggccgcctg tctgagcagc cttaaacttt ttgccgttcg gaattccgcc gccgatgtcc
     2821 tcaatgattt cacgaagagt tgtacccatc ggaatttcaa caaggcctgt gttattgatc
     2881 tttccgccga gagcgaatac cttggttccc gaagacttct ccgtacccat tgagttaaag
     2941 aactctgcac cgtggagaat gatttgcgga atattggcaa aagtttcaac gttattttct
     3001 gttgtcggct tgccgaaaag tcccttaact gccggatacg gcggacgcgg acgcggctca
     3061 ccgcggttac cttcgattga agtcataaga gcagtctctt caccgcaaac gaatgcgcct
     3121 gcaccgagac gaatatga
//
LOCUS       DAWBSW010000223         3084 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5155_length_3084_cov_2.960383, whole genome shotgun sequence.
ACCESSION   DAWBSW010000223 DAWBSW010000000
VERSION     DAWBSW010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5155_length_3084_cov_2.960383"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          847 a          726 c          665 g          846 t
ORIGIN      
        1 gtcagtaaaa gttgctatta acggattcgg ccgtatcggt cgtcttgcat tcagacagat
       61 gtttggtgca gagggttacg atgttgttgc tatcaacgac ctcactaagc ccagcatgct
      121 tgctcagctt ctcaagtacg atacagctca gggcggttac tgtggcaaga ttggtgaaaa
      181 tcttcacaca gtaacagctg atgatgaggc tggtactctc acagttgacg gcaagactct
      241 tacaatctat gctaaggcta acgctgccga gcttccttgg ggcgagatcg gtgtagacgt
      301 tgttcttgag tgcacaggct tctattgctc aaaggcaaag gctcaggctc atatcgatgc
      361 aggcgctaag aaggttgtta tttctgctcc ggcaggcaac gatcttccga caatcgttta
      421 cagcgtaaac gagaagactc ttacttcaga ggacaagatc atttccgctg cttcctgcac
      481 aacaaactgc cttgctccca tggctaaggc tcttaacgac tatgctccta tccagagcgg
      541 tatcatgtca acaatccacg cttacacagg cgatcagatg atcctcgacg gtcctcacag
      601 aaagggtgac cttcgtcgtg ctcgtgcagg tgctgctaac atcgttccta actcaacagg
      661 tgctgctaag gctatcggcc ttgttatccc cgagctcaac ggcaagctca tcggttctgc
      721 tcagcgtgtt cctgttccga caggttcaac aacaatcctt actgctgttg ttaagggtgc
      781 agacgttacc gttgacggta tcaacgctgc tatgaaggct gctgcttctg agtccttcgg
      841 ttataacaca gacgctatcg tttcttcaga cgttatcggc atgaagtacg gttcactctt
      901 cgatgctact cagacaatgg tttccaagat cactgacgac ctctatgagg ttcaggttgt
      961 ttcttggtac gacaacgaga actcttacac aagccagatg gttcgtacaa tcaagtactt
     1021 cgcagagctt gcataagtta ttatcacaaa aagacggggc agaatttctg ctccgttttt
     1081 tcttttttat gattgaatta gcctccccta tgttgtataa taatacacgg acgcatataa
     1141 ttaaagaaag ataaatattt caccgacgga ggtttattat gtatatactt gatgttttga
     1201 aatccattct cttcggcata atcgaaggca taacggaatg gctgccgata agcagcacgg
     1261 gacatatgat tttgcttgaa aagatttgca aattcaagga ggtttcgccg aatttctttg
     1321 aaatgtacct tgttgtcatt cagctcggcg caattattgc cgttgtagta atgttctgga
     1381 acaagctttg gcctttcaag aaaaaacagc ttgaaaacgg caaaagcaag gttgccgtta
     1441 agccgagaat aatcgagctt tggttcaagg ttatcatcgg ttcgattccc gccggaattc
     1501 tcggaattat tttcaacgac cttctcgaca aatatcttta caactatgtt acggttgcca
     1561 taacgcttat aatttacggc gtactcttta tcgctgtgga aatctataac agaaagcgcc
     1621 gtccgaaagt tacaaagctc actgaggttt cgttcaaaaa cgccttcttt atcggctgct
     1681 tccaggcact ttcgcttatt ccgggaacct cgcgctcagg ctcaacgatt ctcggcggta
     1741 tcatgctcgg caattcaagg agagttgctt cggaatttac cttctttctc gccgttcccg
     1801 ctatgctcgg cgcatcgctc atcaagataa ttaaattcgg cctcggattc accgttaccg
     1861 agtttgttat actctttgtc ggaatgataa ctgcatttac cgtttcactt gttgttatca
     1921 gatttttgat gaattacatt aaaaaccaca cgttcactgc attcggcata taccgcataa
     1981 ttctcggcgc aattgtactt atcacctatt ttgttacaaa ataacggagg aaaaatggaa
     2041 ccgcaaagac gttatatatt tgaaaatatt gaaaactgcc gcgatctcgg cggctaccct
     2101 tcaaaatacg gctgcacaaa attcaagcgc tttattcgcg gcggcactgt tgacagaccg
     2161 accgaaaacg acattgaaaa gctcaaggcg ctgaatgtca caaccgtaat agaccttagg
     2221 ggagattttg aattcatcaa tcaaccgaac ggaacggaaa ggctgaccga caatgttgag
     2281 cacatttcac tctatgaaat gaatgttgcc gaagcaaagg gcataaaaaa agctcttaca
     2341 gaggtttatg agtacattat agataattat aaagaaaatg tatgcaaggc gttaaagtcc
     2401 gtcgcagacg cccctgacgg cgctgttttt tatcattgct ttctcggaaa ggatcgtacg
     2461 ggaatactct caatgcttct gctgacgata gcaggtgtcg aagaagatga catcgttgcc
     2521 gactatcagc ttacctatac atacctcgaa aaatatatgt gcacacacgc cgatacgctt
     2581 tgggacacag accctgagat gcactattcc ctgcctcaaa caatgcgctc cctcctcgcc
     2641 tacatcaaaa ataaatacgg ctcggtcgag gactatatcc gacaaatcgg cataagcgac
     2701 agggacaaag aaaagataag aaaaagattt tattgataat aaataactga aagctgccga
     2761 gattcaatcg gcagcttttt tcggaaacaa atatagtaga atattttacg tttgcaaact
     2821 ccaccggact gttttcttaa cgctcagacc ttctcaggtt caagtccttg gcaaagaaaa
     2881 aaaagatcct caatcggctc ttttttcttt ggtcgaggcg acaggacttg ccgtccgctg
     2941 cggctgcgcc ttgcttacta atggctcgac gaccgtcgca agcgacagta cccgtctcgc
     3001 cgctcctcgc taaaaacagt ccaccggact gtttccttaa cgctcagacc ttctcaggtt
     3061 caagtccttg gcaaagaaaa aaag
//
LOCUS       DAWBSW010000224         3046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5239_length_3046_cov_3.059178, whole genome shotgun sequence.
ACCESSION   DAWBSW010000224 DAWBSW010000000
VERSION     DAWBSW010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5239_length_3046_cov_3.059178"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          930 a          637 c          686 g          793 t
ORIGIN      
        1 atgctctata ttttcacaaa gaaaaagggc aaggagcttg ccgaagctat cactaagaga
       61 ggtcacagag gtgcgacgat tattgagggc aagggcggat acacgggaga agtcagcgaa
      121 cttgttatct gcgctgcgag aagccatgag attccgaata ttaaaagaat gtgcaaggag
      181 atagatccgg atgcatttat tgttatgtcg gaggccaacg aaattctcgg ctacggcttc
      241 aatccgccgg ctctggacga ttaaacagat taaattgaat aaagcagaat tgacaagcgg
      301 atattatatt gacaaaacag atttattgca gtaaaatatt tccgtaaaaa ggggcataaa
      361 aatgacgcat agtgcgctga aaaatcgccc aaaaacagga ggaaatgcaa tgaaaagaac
      421 agcgaaaaga attctataaa cacttttgat tgttgttttg tttgtcggtg ttgcagcgat
      481 gggcggaatt gatttcacac caaaagcaaa ggctatggat ttatcgtcgt gcaaggtcgg
      541 tgatttaatc gaattcggtt catatccgca gagcaaagtt acggacagta atctcattgc
      601 aaagattgaa gcagccagtg aaaatgaagc ttgggtggat tataactact acgctggtac
      661 aggcaattgg gcagacgtca acatgaagcc tgttgacgga atgatgctat ataaggatat
      721 cccttacaat ggcaacaaat atcgtgcagt taaaataaat caataccgtc cgtattgcac
      781 gggctatacc tcttcagata catatcaaga tgacaacggt tattatatag gcaatacata
      841 ctattttaaa tatgagcctt taacatggcg agtccttgtc ccgagcgagg gccatgtaat
      901 gtgcaataaa attattgatt cgcaggctta tcagaatttt atttattata acggcagtga
      961 atattacaac agtaaaggtt gtgcaaatta cgcttccgat tgggtgacaa gcacactccg
     1021 tcagtggttg aacaatgatt tttataacac ggcattttct gcagaagaaa aggcacaaat
     1081 cggaacatca catcttgaaa ataagagcac atatgacagc gcagatacat acgacaaaat
     1141 ctttctcatc tcatattata acgcaacaaa cagcgcatac gacttcaatt ctgacagaac
     1201 agcttatgat acagcacgac agatgaaggg cactgattat gcaaaatgtc agggcttatg
     1261 ggttagcaca agctcaagtt acagcggaaa ctcttggtgg tggctgcgct cgccctacaa
     1321 atccttcatt gctacggatg tcgatactgt cggctgggcg tactactact atgtctatta
     1381 cactgacttt ggcgtcgttc cggcttttaa attcaatcca aaacctacaa tctgcgatgt
     1441 tcatgattac aaggctgttg tgaccgaacc aacatgcacg gagcaaggat atacgactta
     1501 cacctgcaca aaatgcaatg acagttatgt tgataactat accaatgcgc tcggtcatga
     1561 ctttggagaa tggaagctta caacgccggc aacctgcaca gaaatcggcg ttgaaacaag
     1621 atactgttct cgctgcaacg taacgcaaac aagagatgtt gataaagctg cacatgtatt
     1681 cggcgaatgg aagatcacta ccgctccgac aacaatttcc gacggagtga aaacacgtta
     1741 ttgtaaaaat tgcgacgctt ttgaaaccga aagtgtcggc aagctcaaat caatatccgt
     1801 gaagctcaat aatattgaga caaactataa gaagtccgga cagcttgagc cgaaaatcac
     1861 taatcccgat aatgtaggct acaagatcga gtataaatcc tccgacccga aatgcgttat
     1921 cgttgacgcc gacgggaatt atttcgccgt taaaaccggc tcgtcaaaaa taacctgcac
     1981 cgttaccgat gaatatggca aggttacgac cgccgagtgt aaagtgaacg ttaaatatag
     2041 cactattcag tggataatta tgattgtcct ctttggttgg atttggtatt aagcgtatag
     2101 gtgttaaatt attatagctc ctcctttcgg gggagctttt gctttaagtt aaaagaaaaa
     2161 ttcaaaggtg cgatgtgggc agaaattatt atttattcaa acgataaatt ggtcatataa
     2221 acaatggact gctccaagcg gaacagtcca aaaattttta tcggtcatta tgctttcaaa
     2281 ttatgagcct taacatagtt aacgacctca tcaacggttg tgaatgcgag tgcaacggaa
     2341 gtgtcggcag caccgtaata aattgcaatt tttccacttt tgctgtcggc aagagctgcg
     2401 caggggaata caacgttcgg aacatctccg acacattcgt atatttcatg tggagcgagc
     2461 aaaaagctgt ctgcccggta aagcaccttc caaggctcat ttttatcgag aattgccgca
     2521 cccatgctgt aggtgaagcc gttgcagctt gtcagtacgc cgtgataaaa catcagccag
     2581 cccttgtcgg tttcaatggg cgtcggacct gcgccgacct ttgtcatttc ccaattcttc
     2641 acgggactca taacgaagcg gttcttgccc caatattcga gatcacggct tctcatgagg
     2701 aaaatatcgc cgtagggcgt gtgtcctctg tcgcagggtc ttatcaggag catgtattcg
     2761 ccgttaattt ttctcgggaa gagaacgccg ttccttgaaa cgggatagct ggcttcttcc
     2821 agctccgtcc agtcctcaaa gtctgttgtg tatgcaattc cgataaccgt accgtgcgga
     2881 gtgagcttga cccaactgag aaagtattta ccctcaattt cgcaaagacg gggatcgtag
     2941 ccctcaaaga tatatttatc ggtgaacttc cagttgattg cgtcgctgct gaatcctgta
     3001 acgagcacct ggtcacgggt tcttgtatca accctgaata cgcccg
//
LOCUS       DAWBSW010000225         3029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5276_length_3029_cov_2.388702, whole genome shotgun sequence.
ACCESSION   DAWBSW010000225 DAWBSW010000000
VERSION     DAWBSW010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5276_length_3029_cov_2.388702"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    398..407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2760..2769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          814 a          688 c          676 g          831 t
ORIGIN      
        1 agattaccga tattaacgtt cagccgattc tcgacgttat tgaaatgggc tatattcctg
       61 ttatttcgac cgtaggtttt gacgatgacg gcaacacata caatataaat gccgacacgg
      121 ttgcggcggg gattgctggc agactccaag cggaaagtct catttccatg accgatattg
      181 cagggatcct cagagataag gacgacccga caactctcat ttcaaagatt tttgttgagg
      241 atgctccgaa gctcatggac gacggaataa tctccggtgg catgattccc aaggtgcaat
      301 gctgtgttga cgccatcaat ctcggcgttc gaaaagtttt tgttatcgac ggacgaattc
      361 cgcattcaat tataatagaa gttctcaccg acgagggnnn nnnnnnnagc gattctcaag
      421 gacagcaacg gaaaagaata tattgatctc ggcagcggaa ttgccgtaaa cattttcggc
      481 gttgccgatg acgaatggca gaaggctgtt acccatcagc tttccgttct cccccacacc
      541 tcaaacctct actattccga gccctgctca actcttgccg agcttctctg cgagagaacg
      601 ggaatgaaaa aggttttctt ctcaaacagc ggtgcagagg caaatgagtg tgcaatcaag
      661 gttgcaagaa agtatgcttt tgataaatac ggcgacgaga gtcactcaac aataatcacg
      721 ctcaagaaca gcttccacgg cagaacaatc gcaacgctca aggcaacggg tcaggatgtt
      781 ttccatacgg atttcggacc gttccccgag ggctttgttt atgccgaagc caataatttt
      841 gacgacgtaa agaaattcgc cgacaccgac aagtgcatcg cagttatgat ggagcttgtt
      901 cagggcgagg gcggcgtttg caagcttgat gagaatttcg tcaagtcggt tgcagagctt
      961 gttaaaaaga aagatatgct tctcatcatc gacgaggttc agacgggtaa cggcagaaca
     1021 ggtgagcttt acgcctatat gcactacgga attcagcccg atgttgttac aacggcaaag
     1081 ggtctcggcg gcggacttcc gatcggagca acaatgctcg gagaaaaagt cgagaacgtt
     1141 ctcaccttcg gctcacacgg ctcgaccttc ggaggcaatc ccgtaagctg tgcaggtgca
     1201 tgcaacatca tcaagagaat tgatgataag cttctcaatg aggttaaaga gaaatcggcg
     1261 tatatcttct ctgagcttga gggtgcaaag ggcgttaaat ccgtcagcgg tctcggactt
     1321 atgatcggcg tcgagacaga aaagccggca aaggaaattg ccaatgcctg cattgaaaag
     1381 ggtgtgcttg tcctgacagc aaagacaaag gtaagacttc tcccctctct caacattgat
     1441 tgggacgatc tcaaaaaggc aattcagata ctcaaagaag ttattgcgga ataattttaa
     1501 ataaaataat tgcagccaca gttcattttt taactgcggc tgttctttta taaattcacc
     1561 acattatatc ttgcaattct gcacaaaata catatgttgc tatttgtgaa ctttgtatag
     1621 caaattgtgc cgtaatatgt tataatatca tgcaatcgaa aggagaggac gttttggaag
     1681 aaaacaaaat gggcgttatg cccgtaaaca aacttattat caatatgtcg ctgccgatga
     1741 taatctcaat gctcgttcag gcactgtaca atattgttga ctcggttttc gtagccatgg
     1801 tcaaccagga gtcgctcaca gcggtttctc tttcattccc tgcacaaaac ctcatgatag
     1861 gctttgcaac aggtactgca gtcggcgtaa atgccctcat ctcccgtgca ctcggtgaaa
     1921 agaacagtga acgtgcaaat aaaatcgccg agaacggtgt ttttcttgca ctgctcagct
     1981 caatagcttt tttccttttc ggcgtgttcg gttcaagact tttcatcagc tctcagacct
     2041 ccgtgcagta tattattgac gagggcacaa agtatcttca ggtttgctgc ggcgcaagct
     2101 tcggcatttt ctttgagatc atgtatgagc gtcttttgca gtcaacgggc agaacctttt
     2161 acacaatgat aacccagggt gtcggcgcaa tcattaacat cattcttgac ccggtattca
     2221 tttttgtatt taaaatgggt gttaaaggcg cggcgatcgc aacggttatc ggacagattg
     2281 tagctttcat tctcgccgta tatttcaacc gtcacaaaaa cccggatctt caccttgatt
     2341 tcaaaaagtt taagccgagc ggacgcttca tcggcgagat ttataaaatc ggagttccgt
     2401 caattctcat ggttgcgatc ggctcggtca tgacatattt catgaataaa atcctaattg
     2461 catacactct cggcaaggag ctttccgtca atgttttcgg cgcattcttt aagctcaaca
     2521 gctttatttg tatgccgata ttcggtctca acaacggcgt tattccgata atcgcctaca
     2581 actacggcgc aatgaaaaag catcgcatga taaaaacgat caagctttct gtcgtgtatg
     2641 ccgaagcatt cagcattatc ggtgcaattc ttttctttgc attgcccaag gttttgctgg
     2701 gatttttctc ggcaactccc gatatgctca gaatcggtat cccggcgttg aaaataatcn
     2761 nnnnnnnnnc aagaaaaact gccatgtctt ttaccgaact gacttccttt ttgcaggcgc
     2821 aaatattgcc ctcagcggcg ttttccagtc cttcggaaaa agtattttct caatgataag
     2881 ctcatttatc cgccagcttg tcgtgctcat tccaatcgcc tatatccttg cgagatacgg
     2941 tctgtcaatc ggcaatgaca acctcgtttg gtggtcatat tcaatcgccg attttgccgc
     3001 atccgttgtt tcaatagtct gttttatta
//
LOCUS       DAWBSW010000226         3028 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5279_length_3028_cov_3.103935, whole genome shotgun sequence.
ACCESSION   DAWBSW010000226 DAWBSW010000000
VERSION     DAWBSW010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5279_length_3028_cov_3.103935"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2788..2797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          719 a          721 c          593 g          985 t
ORIGIN      
        1 tgccttttcc ttcgctaaac gttctttttc tgcctgtgcg gcctttgctg cggcatcttt
       61 ctgttttgct ttttcttttg caagacgctc tttttctgcc tgtgctgctc tggcttttgc
      121 aattgctgct ttttctctct gtttggctgc ggcagccttt tccttggcct tctgagcggc
      181 aagctctttt gccttgcgct ccctctcttt agcctttgct tttgctgctt tttctttctc
      241 tatcttagca agctttgcgg ctttctctct tgctatgcgc atttcctcac gctgcttctc
      301 ttttgcaata cgagcttctt cctgcgcttt gagtcttgcc gctctttctt tttcttttct
      361 tgccttctct tttgcgagag ctttctcttt ggcgatacga gccttctcgc gttctttctc
      421 tctcttgatt ctctgtctct ctttttcttt ttctttcctt gccctctctt tttcccttgc
      481 tatgcggcgg cgttccatct ctttttcatg agcaattttc gccttgatct tatcttcaat
      541 ggccttctta cgcgcatttt ccgcatccaa cgcacgctga atagcctcct gaattttctt
      601 ttcaccctca attttcttgc ggctgacacg cttgacctca tcaacaaaga cacgctggat
      661 ttcaacttcc tccatgtcat agtccataac aagctcttct gccagtctct tgaggagttt
      721 tggcttaagc ttacgctttt tctgctttcc gacggaatcc tcagagaggg tgttcgcctt
      781 gagcatcata tagttcaacg ctgtcatgcg attgagttcg ttcatatact ctgcgctgac
      841 catatcattt gattctttga cggttatttc ataacccaac tcgttgtagg actgaataaa
      901 ctgccacaat ttcagacaag ctctgaaatt cgggtttttc tgaatagcat ttgttctcat
      961 gataggcggt cttacgggaa cgcagttcct gagttccttc ataaatgaag aattagcata
     1021 ttcgttaata attctcttta ctctttcaat gcgctggaaa gcagtggcgt tctctccttc
     1081 cggacccgct tcgccttcaa tttcgctgcc ggcagactga gccgagactt caagcttata
     1141 ctgaatcttt tcacgtcctc ttgtaaaatc actttccatt ttaagggacg ccatattatc
     1201 atcgtcggaa atattgaaaa gaacattata tcttacatca ataaatctga cgaggttctg
     1261 cataagagtg aaaacaaatc tgttttcata aacctcgtag ctttcctcac ggaagacgtt
     1321 gagaatctgg ttaggtgtaa cgtcatcgcc ttcaaccttg gcaatcatat ttgtatgctg
     1381 agcaaggtga cggatagatt cgttcgtgat ttttctggca cgttcaatgg gaacgatttc
     1441 ttcctcctga acgataaact ttctcggaac acggataata ttgtcaaggg gaatgatgca
     1501 cgcttcgatc tcctcaaccc acttaaggtc gatcttctta tcaaaatatc tgttgaacaa
     1561 ggaatagctg ttcgtaccgc tgtttatggc agtatccatt tcgctgtaaa actcgttatc
     1621 ggaagttgcc gacgcgagat aatccttgta ttcaagatat aattcaagat acgatttgtt
     1681 cattaagtat gtcccctttg acaaaagtta taataacggt ttcgggatta cataagtctc
     1741 ttaaggagct ggagatactc tttacactcg ttcatgtttg ctttgccgaa gtgcttgtta
     1801 agatatgtaa cgagtccgtc gatttcatcg cggatgaaag aaaggttcaa gctttcaaac
     1861 ttacggaaaa ccttcttggc aagaacaaag tcaatgccat caatttccgt tccgccgcag
     1921 ccgacatatg caggaacgaa ttccttgagc tgtttaacaa tacggttacc gaaagcaaga
     1981 cggaaatgct cgataacata gtcgtcaagc tcctcaatct tcttaaggtt ctcgtcggaa
     2041 actttatact tggtctggca ctcaaggaac atgccctcaa ggtgcttata gctgaggttg
     2101 agctcctctg tgtacggagc ctcaaagaac ttacccttac tgttaaggtc gattggaata
     2161 gcacggtcgt aaaccttatc ggaaacagca aatgtcgagt catcgttatt gattgtaccg
     2221 atataccaca tgttctcggg gagcttgagc ttaccgtcga taacatgctt cggatcggtc
     2281 ggccatacgc tcggaacaag ggaaacaacc cactcgtctc ttgacggcat ttcaagaatt
     2341 gaaagaagct cagcgaaata atactcaaca cgggcaatgt tcatttcgtc aagaacggta
     2401 atgaatattt cctctttgaa tgtagcctca tacatctttt tgagaacttc cgtctcatta
     2461 aaacgctttg tgaattcgtt gaagtatccg aaaatctcgg tacggtcacg ccatgaaggc
     2521 tgaaccgaag caattgtaac agggttctta aggaatttac cccatgcata agcaagtgat
     2581 gttttaccgg tacctgaaat accctgaaga attataagtc ttgttgaagc aaacgcggaa
     2641 aggaacaatc tgatgatctt gatctcataa aaaagaccga gacgtgagca agcaaagttg
     2701 cggaaccttt cgcagatttc cggaagtgtg atttcattat caaaatcggg agcaacatag
     2761 ttctccattt cgaggtcaac ctgagtannn nnnnnnncct cgccgttctc gtcatacggg
     2821 ctttcaccgg ggttaagacc gagctgtgaa accttcatgg caagaattct ctctttctcg
     2881 ttgttgagct taataacctc acgattaagg ttaccgactt ccatgagaag ctcttcctgg
     2941 tcaacgagag tctttcgtac tcttacatac tttttaataa gcacaacaac aagaattact
     3001 atagctgcaa gcaccgctac tgttgcga
//
LOCUS       DAWBSW010000227         2998 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5343_length_2998_cov_2.107034, whole genome shotgun sequence.
ACCESSION   DAWBSW010000227 DAWBSW010000000
VERSION     DAWBSW010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5343_length_2998_cov_2.107034"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    745..754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          872 a          659 c          714 g          743 t
ORIGIN      
        1 ctccgttgca gatcgcccaa atgctcagtg ccgttgcgaa cggcggcaaa tacagagaac
       61 cgtatcttat cgaagccgtg accgaccgta acgggctcga aacaaagcac aaaaagaaat
      121 atccgactgt tgccataagt gaagattcaa gttgtatcct gctgaaaatg ctcacctcgg
      181 tcgttgaaaa cggtaatgct tcccctgccc gtcccgaacg tttttccgcc gcaggcaaaa
      241 cagcaacggc acaaacaggc atttttgaca aagacggtaa tgaaatatgc aacacgtggt
      301 tcggcggatg ttttcccgcc gacgagcctc aatatattgc cgtaatcatg aaacagggcg
      361 gtgcttcggg ttcctatgac tgcgctcccg tattcaaaaa aatagccgac agcataaatt
      421 tttccgaatc aaaaaaatca taaaaaactc ttgaaatagc cgattctatg tgatatactt
      481 ttacggcaaa acgcatgtaa ggtgttttat tcggagtgca ttttgcatga aatgttcgga
      541 tatcaagcct tgcagaggca aaactaagga ggatttattt atgccagtag tatcaatgaa
      601 acagttactc gaagcaggtg ttcacttcgg acaccagaca agaagatgga atccgaaaat
      661 ggctcagtac attttcacag aacgtaacgg aatttacatc atcgaccttc agaagacagt
      721 caagaagctt gaggaagctt atctnnnnnn nnnngcggta tgccgtacgt taatgcacgt
      781 tggctcggcg gtatgctcac aaacttcaac acaatcaaga gaagaatcaa gagacttgag
      841 cagctcaaga aaatggaagc cgacggaaca ttcgatcttc tcccgaaaaa ggaagttatc
      901 aagctcaagc ttgaaatcga aaagctcgag aagttcatgg gcggtatcgt taacatgaag
      961 aagcagccgg ctgctatgtt catcgttgac ccgcgcaagg agagaatcgc agttcaggaa
     1021 gctcacaagc tcggtattcc aatcgttgct attgtagata caaactgtga tccggacgag
     1081 atcgactatg ttattcccgg aaatgacgac gctatccgtg ccgttcgcct tatcgcaggc
     1141 gctatggccg acgctgttat tgagggtcgt cagggtgagc aggaagctcc ggcagaagaa
     1201 gctgccgagg aagctgctgc cgaataatca gaacaaattt aacggcggaa tactatattc
     1261 gtgagaagac cggagcaggc atgatggact gtaagaaggc tcttacagag acagacggcg
     1321 acatggacaa ggcagtagac gtgcttcgcg agaagggtct tgcttccgca gccaagaagg
     1381 caggccgtat cgcagccgag ggcgttgttg ttgcatatta tgacgaggca gccaaggttg
     1441 cttcaatggt agaagttaac tgcgagacag acttcgttgc aaagaacgag aagtttgttg
     1501 agcttgcaaa taagattgcc gttacagttg ccgagaagaa tccggcagac gttgacgctc
     1561 tccttgctat gaccctttcg ggcagcgacg agacagttgc cgacgctgtt caggagcttt
     1621 tccttacaat ccgtgagaac atgaaggttc gccgttttgt aagagttgag ggtgacgttg
     1681 catcctacat ccacggcgca ggcgcagtag gcgtacttgt aaaatttgac acagacgttg
     1741 ccggcaatgc agagttcaag gatatgggca agaacgttgc aatgcagatc gcagctatga
     1801 atcccgagta tctcagcaag gactgcatct ccgacgatga gcttgctaag atgaagtcga
     1861 tcacaatcga cagctcactc aacaagcctg acacacttcc gaagccgatt cttaacagcc
     1921 tctttgctaa ggctgttgac ggcaagcttt tctcagatga ggatcttgca gcctatgagg
     1981 agcagaagaa caacaagttc cttttcaact tcctttcaga caaggctgtt gcaacactcg
     2041 ttgagctcgc tatggcaagc aaggacgaga tcgttgccaa caagattttt gccggcgctg
     2101 ttgacggacg tatgaagaag cagctcaagg aagtttgcct tctcgagcag gcatttgttc
     2161 gcacagacct cttcgacggt aacgttgcag gctacatcgc agacgtagcc aagaagcttg
     2221 gcggttccat caaggctgta agctttgttc gctttgagaa gggcgaagga atcgaaaaga
     2281 gaagcgatga ttttgcagca gaagttgcaa gcatgatgta atttcatcaa tataaaatgt
     2341 atcccctgct tcggcagggg atttttttgt tttcgctgac accttgtcag gcttcctctt
     2401 cgaaaccgtt cgcttgccct ttagtgtgag ctttcacggc tcatccgtta ctgagaggtt
     2461 gattcaagat aaaagtaaag agtgaaaata gtatgtagct ttgtgtattg catgtccgca
     2521 aagtcagctg aaattaacgc gaatgacaaa tggtttccct tacaaaaagc tgaattttca
     2581 aaaataattt aagttagatt taatttcaag atacaatatg ggcattgcac tatatggtaa
     2641 gtgcaaaaaa tgtttttaca cggaaattcg gttagagcac aattcgtatg aattgattct
     2701 aacttcggct caaggaaatc tgtaaataaa aagaccgaca cgaacgtgcc ggtcttttta
     2761 atattattca gcctataaca gtgaccttat cgtaaagctc tttatatacg ccgccgagat
     2821 cctgaacctc aacaataaaa ttcttatcgt caccgttctt gatgctgtag tttgcaatta
     2881 tcgtgcttgc gtttgcttca tcgttcgccg tcattccgca tgacgagagc ataaacaagc
     2941 caattaaaac aacaagtgtc aaagaaacaa atcttttcat tgctcagcct cttatctc
//
LOCUS       DAWBSW010000228         2970 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5412_length_2970_cov_2.207547, whole genome shotgun sequence.
ACCESSION   DAWBSW010000228 DAWBSW010000000
VERSION     DAWBSW010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_2970_cov_2.207547"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    354..385
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          791 a          644 c          669 g          834 t
ORIGIN      
        1 tcgttaagcc cgagttttat caggtgagcg agggaacgct cagagaacgg gcgggaaaaa
       61 tcgcaggaga aacgggtgca ccgccgccga caaagctgaa acgtaacagg ttgattattg
      121 ccgtaaagct tgcggtttcc gcaatttcag gagcggccgt tgcattggcg atcagtcgat
      181 tcatatttta aaaagctaat ctgacgaagc ttttaacttt aagagcaata tcgtatattt
      241 taattatgcg atgttgcctt atttttttgc cgatttgcaa aagtccgcca tacagcattt
      301 ttcacaggca ggacgtcttg caacgcacac ggctctgccg tggaggacac agcnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnagacc gaggaggttg cttatacgaa taaggtgagt
      421 atcggcaaca acagcaggct tgccgaatat gtcgccgcaa atcaaatttg ccgttttcct
      481 gccgactccc ggcagagagg tcagatcctc aatgttgtcc ggaaccatgc ctccaaaatc
      541 ggaaagaatt ttttgcgcca taccgataat gtccttagcc ttggtgcgat aaaagccgca
      601 tgagcggata atctcctcaa catcggcaac gtcagcctcg gcaaatgctt cgaccgtagg
      661 atatttttca aagagtgcag gcgtaacgat attcacacga gcgtccgtgc attgggcaga
      721 aagtctcgtc gcaacaagaa gctgaaaatc atttttataa ttcagagagc aaattgcgtc
      781 gggatactct ttcttgagtg cctcaacaca aatcagggct ctttgctttt ttgtcataac
      841 ctcacacctt tcctgaaagg taaaattccg ttaaatgaat tgctttttgc agcaaattgc
      901 cgccgaggga tttaaaatgc tttgatcggc ggaaatttaa aatattatac tacattattt
      961 aactaaaata aagggctttg tggataattc ggcggtattt ttgaaccgtg aacggtggcc
     1021 gagaaaaaaa gtgaagttaa tcctcggtta ttgcttttta tcgttttcaa attatattta
     1081 taacatgaaa aagaaaatca gccgtcaaat gtaaaataat tccaaacata ttgagatttc
     1141 ccgacaaatg cgttataata aattgattta ttatactcac aagggggaat tatgtgagcg
     1201 gagaaaaact gaacaataag ttccactggg gatttgctct tatcctcgtt acaatatttc
     1261 aagtgttgac cggaacagtg gtaataaccg ccaacggttt caacagcact ctgtttgcta
     1321 tctttgcagg ttatattctt cttgaatggg cttattttat cattgcttct attttttgcg
     1381 gacagaataa ctttgagctt gagataatcg gtttcctctt ctcgggcatc ggtctgacga
     1441 atgttgccac agttgataac gactatgctc tcaaacaggt cgttgccatt gcgctcggac
     1501 tgattctgtt tatcttcatg ctcgcggtta tgcgccgcac ggatatcgtt atgaaactgc
     1561 gaatgcccgt tgcctttgcg gcggtcggac tgctcgtcgc caaccttgcg cttgcaaaat
     1621 atacgaacgg tgcgcttaac tggatcacaa taggcggcat atcaattcag ccgtccgaat
     1681 ttgtcaagct tgcgtttatc tttgtcggag cggcgtcgct ggacaagctt caaagtgcac
     1741 gaagccttac taaatatctt atattctcct tcggctgcgt cggtgcgctt ttcctcatga
     1801 aggacttcgg taccgcattg attttcttct tcacctttat agtaattgca ttcatgcgct
     1861 cgggtgacgt cagaacgatt gcacttgtat gcgttgcggc tttactcggc gcaataatga
     1921 tcgtatattt caaaccgcat gttgccgccc gattcaaggc ataccgtcat atctgggagc
     1981 ttatcaacga cgaaggtatg cagcagacga gaactcttat ttattcggca agcggcggtc
     2041 ttttcggtct cggaataggc aagggtatgc tgagaaatgt tgttttcgcc tcgaccgacc
     2101 ttgcgttcgg aatgatatgc gaggaatggg gcatgataat cggtctgact atagttatca
     2161 catatgcttt tattcttctt tatgcaatca aggttgcgag aaacacccgc tcggctttct
     2221 atgcaatcac agcttgcgcg gcggcaggac ttttgctgtt ccagacaagc ctgaatattt
     2281 tcggcgtaac ggatatactt ccgtttacgg gagttactct tccgttcatc agccgaggcg
     2341 gatccagcgt tatttcctca tggggcatgc ttgcactgat aaaatcggca gatatcagaa
     2401 cctatccaaa gctttccaag actatacttc ccgatcatcc gctttatccg cctaatataa
     2461 tgataggcaa gaggtccaag cgaaaggtat atgttccgcc ggttgttgag cagtcggtca
     2521 gagtcaaaag ctccgcagcg gatgtgccta taagcaaaaa acggacacag caagccggca
     2581 ggggcgatgt tcccataagc aaaaagagaa caacctctgc tcagaattca aaggacattc
     2641 cgatatcacg gaagaaatcc gggaacggga gggctaaata aatgagaaat accacaagac
     2701 gtgcctttgt tctttacttc gttatagttg catttttctg cggactcgga ttcatgatgt
     2761 acaatttctt tgttcacggc tcggaatgga cgtcaaatct tgttaatcag catatttata
     2821 aaaacggcga aatttccagt gccggtacga tttttgaccg taacggaaca aaactcgttt
     2881 attcggagga cggtgtacga atttataacg atgacaaaac aattcgcaaa gccatgcttc
     2941 atgctgtcgg tgacaattca tctttcatcg
//
LOCUS       DAWBSW010000229         2966 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5422_length_2966_cov_2.142906, whole genome shotgun sequence.
ACCESSION   DAWBSW010000229 DAWBSW010000000
VERSION     DAWBSW010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5422_length_2966_cov_2.142906"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    839..848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          713 a          705 c          665 g          873 t
ORIGIN      
        1 cgttgatatc ggtaatctcg ccgacgtagc cgagctcagc ggctttgggc ttcgccttaa
       61 tcattccgcc gtccgaaccg ctcagaccga ttgcttttcc tcctcttgca cagagcaggt
      121 tgacaaggct tttgtttatc ttgcctgcga gaaccatctg aacgacatcc gctgtttcct
      181 tatccgttac acgcagaccg ttgacaaatt cgctcttttt gccgattttg ttcagcatgc
      241 ccgtaatttc gggaccgccg ccgtgaacga gtacgacctt gattccgatc gtcgaaagca
      301 aaataatatc gctcattacg gattccttca gctcttcgct gaccatggca ttgccaccgt
      361 atttcacaac aaggattttt ccgttatatt tttgaatata ggggagagca cgggtaagaa
      421 ttccggcttt ctcttccatt gaaatattca tctgtattca cctcaggttc tgtagtcacc
      481 gttgattttt acatattcat atgtgaggtc gcagccccat gccgttgcgt cgaaatctcc
      541 gtcgttgagg tcaacaagaa tttcgatctc cttttcaaga aggatttcct ttgccttttc
      601 ctccgagaag tcaacgccag caccgttgtg gcaaacggcg attgtgcctt taacggactt
      661 gaagctgacg tcgatcttat tgatgtcaac gtctgctccg ctgtagccga ttgcgcaaag
      721 tacacgaccc cagttggcat ctgcaccgaa cattgccgcc tttgtaagcg atgagcaaat
      781 aaccgacttt gcaacttttt ttgcgatttc atcggtttta ccgcctgtta ccttacatnn
      841 nnnnnnnnat tcaagcagct tggttgcacc ctcaccgtcg cctgcaattt ttcttgaaag
      901 ataaacgctg acctgatgga gagccttgca gaatttttca taagcctcgc cctcgccgtc
      961 aatgagcttg ttacctgcca ttccgttggc gagaacagca aacgtatcgt tggtcgatgt
     1021 gtcgccgtca acgcttatca tattaaatga agtcttaacg tcctcgctga ctgccttttg
     1081 aagcatttcg cttgtgattg caacatccga ggtgacgaaa acaagcattg ttgccatatt
     1141 cgggtgaatc attccgctac ccttggcaat tccgccgatt ctgcattcaa cgccgtcaat
     1201 gtcaaagctg actgcgactt cctttttctt cgtgtcggtt gtcatgatgc cttctgcggc
     1261 aagaccgctg ttgtcgtagt caagacctgc aaccagctcc gccataccgt ttgcaatagg
     1321 agtgaggttg agcggcatac cgataacgcc tgttgaacct acaataacat cgtccttgtt
     1381 tatgccaagt gcgtcctcaa cgagccgacc catggcttcc gctttttcga ttccgtcggc
     1441 gttgcaggtg ttcgcaatgc cgctgttgca gatgatagcc tgggcatgtc cgtcggcaag
     1501 gtgcttctga ttgacaaaaa tcggagcacc cttgacgaga tttgttgtat aaacagccgc
     1561 cgcagttgcc tttacgtcgc tgacaatgag cgaaaggtca cgttttgact tgttctttct
     1621 gatcccgcag tgaattccgt tggctttgaa gccttttgcg gcgcagatgc cgccttcaat
     1681 tgttttcatt atttttatac ctcgagtcct gttttttcgt ctgttccgag aagaatattc
     1741 atgttttgta tagccgctcc tgaagcgccc ttgccgagat tgtcatatct tgacatcaaa
     1801 aggattctgt cctcgttgcc ttcaacaaag atttccattg agtcattgaa cgagagctta
     1861 ttggagcaaa tgaaaccgtt ctcgccgatt tcgtctttgt atttcacaat atccgaatga
     1921 tatttgtctg catagatttt cttgatgtcc tcaattgtaa cgccgttgag ctgttccttg
     1981 aaaagaggaa cggtgacaag catgccgctg taataatcgg caacgatcgg gcagaaagcc
     2041 gggaaagcat tgattcccgt aattgctttc atttctttaa ggtgcttatg attctgcgtc
     2101 aagccgtact gcctcggcga ttcgagtgca acatcgggct cggcatcctc gtattcggca
     2161 atcattttct tgccgccgcc gctgtagcct gtcagcgaat ggcaggtcag aagcgtgtcg
     2221 ggagagatcg caccgacttc gacaagggga tagacaagtg agatgaagcc gcttgcatgg
     2281 cagccgggaa cggcaattcg gttggaattt tttatttttt ctctgtgtgc cggagaaagc
     2341 tcggggaaac cgtatgccca gtcgggattt gttctgtgag ccgttgaggt gtcgagaact
     2401 ctgacgtttt cgttctcaat caatccgacg gattcaatag ccgccgcatc gggcagacaa
     2461 agaaaagcga tatcacagga gttaagagct ttgcgtcgag tctcaacgtc cttgcgtttc
     2521 tcatccggca gtagcataag ctcgatatcc gatcgttcgg atagtctttc ataaattctg
     2581 agacctgttg ttccggcctt gccgtcaata aaaacctttt tcatgcctta aacctctgaa
     2641 tctgttaata gggtataatt atacataaat atacagaata tgtcaagaat aatacaaaat
     2701 atattcattc aatataatac aaatattcgt gtggaaaacc gcaataaact gggaattatg
     2761 ccgttgagag gcttgataat ttgtaagaaa agagaaatga atattcattt atattttaaa
     2821 cgggcaaatc gcagcattgc attataagcg aatttaacct ttataattat aactcagacc
     2881 gtgacaaaaa acaaccgtta tttgtaaaga aaatgaaata gtttgcgtga aggtatattg
     2941 atggggcggg cgatgaatgt cagcag
//
LOCUS       DAWBSW010000230         2946 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5457_length_2946_cov_2.545832, whole genome shotgun sequence.
ACCESSION   DAWBSW010000230 DAWBSW010000000
VERSION     DAWBSW010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_2946_cov_2.545832"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    358..482
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          760 a          621 c          709 g          731 t
ORIGIN      
        1 cctttttcga cggatttatc atctcggcgc gctataatga aataaaaccg gcggagaaga
       61 tttacaggat tttgtttgat gagttttcac tgattcccga agagtgtttc ttcattgacg
      121 attcgcagac gaatgttgat atggccgaat atgtcggaat gaaagctcat tgcttcggcg
      181 atcgtgatat tgaaagactt aaacaagata tgagaaacaa caacatagta atttaattag
      241 aattggagct aaaaatgtcc tgttttacaa aagctctgaa gggtctcggt gagtatcgct
      301 ccctgcttta taatgtgaca aaccgacgct tgcctgcggg tgcgctcgga ctgtcgcnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnattcacaa ggcgcacgtg atatcggcgc tttgccaaga cctttcacct cacaaagcaa
      541 tagttataac gcccgacgaa agctcggcga ataagctgtg taaagatctc aatgcctttg
      601 gctgtgatgc cgtcatgtat cctgccggag acatatccct gcgccccgac gacataaaat
      661 cccgtgatta tgaacattcc agactgagcg tgctcggtca tctcgtcgat ggcagtgtgc
      721 gtgcggttgt ctgctcggtt gaggcggctt tgcagctcac aattcctcct gaggagctta
      781 tcgccaagag gataacgctc accgtcggcg aaaccgtcga gcaggatgag cttattgaca
      841 ggctcatcaa tgccggatat acaaggagca gccaggttga cggctcgggt cagttcgccg
      901 tcaggggcgg aataattgat atcttttctc ccgactgcgc taacccttac cgagttgaat
      961 tctggggtga cagtatagac tgcctttcta cgtttgaccc cgagagtcag cgacgtatag
     1021 attcgacgaa ttccgttgta attacgcctg caaacgaaat ctgctgtaat cccgtcagac
     1081 ttgccgacct tattgaggag cagattgcaa agacaaaggg caaaggctcc gttcaggcaa
     1141 agcagttgct ttctgccgac cttgaaaagc tcaacggcgg aattcatctg ccgaatatcg
     1201 acagatattt gccgctcatc tatccctgtg cggcaacgat ttttgactat attgacgagc
     1261 cgcttttgtt tgtcagcgag agcttcgccg ttaaagagca tgcgaacgct tcgcttgagc
     1321 ttttccatga ggagcttaaa gctttccttg aggagggcat catttgcaaa ggtcttgaca
     1381 gatttatgct cgaattcagt gagcttttgg gcatttatga ggacctcggg gcggtctatc
     1441 ttgacaacat tgcaagaggc tcgtttgata cgcctgtcaa gggacttgta acgttcaatg
     1501 cacgtcagtt ttcgccgtgg aacggttcaa tgagcgtgct tattgacgat attaagccaa
     1561 tgtatcaaaa gaaaggacac tcggtttttg ttatagcggg aacagataaa tcagctcagg
     1621 cattggcatc ggatttgcag ggtgaaggct tttctgcgac atatatgcca aacccgccag
     1681 aggagctgat tccgaacaaa gtcattgttt tacccggcgg attttcagca ggctttgaat
     1741 atcccgatgc cgggctgaca gttataacct acggaagcag aacagctctg cctgtcagaa
     1801 aggctaacaa aaaagtcaac aaggcggcgg tgttcaattc gcttgaggat cttcacaagg
     1861 gcgattatat tgttcatacc gctcacggta tcggcatttt tgagggtatc acaagccttg
     1921 aagccgacgg aaatattaag gattatatca aaataaaata cgacaagggc gacattcttt
     1981 atgtgcccgt tactcagctt gatcaggttt cgagatacat cggttcaaaa agtgagaacg
     2041 gcacggtcaa attaaacaag ctcggcggca aggaatggca gaaaacaaga tcccgcgtac
     2101 gctcggcggt taaggatatc gctcaggagc ttattgacct gtataccaag agaacacaga
     2161 taaagggttt tgctttttct cccgatattg atatgcagaa cgattttgag cgccgttttg
     2221 aatatgagga aacggatgat cagctccgct gtattcagga gataaagaac gacatggaga
     2281 agccgtaccc gatggacaga ctcctatgcg gcgatgtcgg cttcggtaag acagaggttg
     2341 cgctgcgtgc tgcgtttaag tgcgttgccg acggtaaaca gtgtgcgatt cttgtgccta
     2401 caacgattct tgcactccag cattaccaga cgatttgcaa gcgttttgac ggtttccctg
     2461 ttgaagcaca gatgatttct cgctttgttc cgactaatca gcagaaaaaa atcaagcagg
     2521 atctcaaaag gggatatgtt gatattattg tcggaactca caggctgatt tcaaaggata
     2581 ttgaattccg tgacctcgga ctgataattg tcgatgagga acagcgcttc ggtgttgcgc
     2641 agaaagaaaa gcttaaagag caatacccga atgttgatgt attgacactc agcgcaacgc
     2701 ccattccgag gactctcaac atggcaatga caggaatcag ggatatgtcg attcttgagg
     2761 aagctccgca cgacaggcac cctgtccaga cttatgttat agagtataat tttgatgtta
     2821 taattcaggc gattcaatct gagctgcgcc gcggaggaca ggtttattac ctgcataaca
     2881 gaacggaaac gattgaaagg atggcgtcaa agctcagaga ttttctcccg gatacaaatg
     2941 ttgccg
//
LOCUS       DAWBSW010000231         2938 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5472_length_2938_cov_2.327784, whole genome shotgun sequence.
ACCESSION   DAWBSW010000231 DAWBSW010000000
VERSION     DAWBSW010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_2938_cov_2.327784"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    957..966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2096..2105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          760 a          650 c          585 g          923 t
ORIGIN      
        1 aagcaacctt ggtctgaccg tcgagcttat cgacaaatgt tgccttccac tcaatataat
       61 atccctttgt tccggcggca agatagggag aagaactttc tctcgtgatc gttgtatcaa
      121 tcttgttgcc cgccttggta taatagatgt atgacgcagt accgcttgtg ttataagctc
      181 tgagcggtgt ggcatactca tcaagcttga tgttacaatt cgcatttgcg tagtcgcccg
      241 atgaggtctg gctcgtctga gtataagcgg tcatatcggt catgctgttc gtaagatatt
      301 tgtacgatac agtaaccgag ctggcattct cataatagaa gtaaaaatta ccggtagaat
      361 tttcacctgt tctgagcgtc tgcagttttg ttgagtcatt taccgttgag tcaacaaacc
      421 actgataatt gtatttgtcc tgggttgtat atcctgtgag cttaggctca agatatattt
      481 gctccggggc atagaaatac acattagaag cactgttgtc agataccgtg attgccgagc
      541 ttgcgtcaac agcagagccg aaaagcgctc cgatatcaaa gcaaataaag gtggtcagca
      601 taagcgttaa agccagaatg acagataacg cttttttgca cgttcgatga actttcttca
      661 ttaaaactcc tccttataaa tgagaatatt ccggttattg tgattatagc atttttattg
      721 tgaatagtca atagacatta tatctatttg tcacttttta agcatataca atcataaatt
      781 ttgaaaatat atgacaaatt caataaaaat attataatta taagaatatt ctcacataat
      841 cgtgaaatgc tactgtcgag aaacatttag gagatgaaaa aatgatagat aagatctcat
      901 tgatattggt catcatcggg gctgttaact ggggttcaat cggacttttt aagtttnnnn
      961 nnnnnnatcg tgagcagaat catttttacg attgtcgcac tcgccggtat ctggtgcatt
     1021 tcgatgcttt tcagagacaa gagagaggtt gaggtcggca cctcgatgga ataagagatc
     1081 aaaaacgcag ggcgcatcaa acgtcctgcg tcttttttgc agcgccgtac gattgaggtg
     1141 tacggagcgt gcaagctgtt tttattttct gcttttataa tccttgtcga tttcttcgct
     1201 ccagcccgac ggcagcggag ttgattctct tatgtgttta accgcttttg aaaccgcttc
     1261 atacactatc gccgttccct gactgtccgc attataattt acggctctgt cgctgtcgat
     1321 tccgaacctg tcggctgttt cgactgcatc aatattagct cctataaaaa tgaattccca
     1381 gccgtatttt gacttctgtc ttttgattgt tcttctgatg ctgtccgccg aatacaggtg
     1441 gcttgcgttt tcatagccgt cggtcattat gacaaacatt gtgtgcgccg gaatgtcctc
     1501 ttctcttgca tatttatgta tattgccgat atggtgaatt gcgccgccga ttgcgtcgat
     1561 aagagccgta cagcctccga ccgtataatc gctgccggtc atctcagata cgttttcaag
     1621 cctgaccctg tcatgcaaaa cctttgtgct gtcattaaaa agaacagtcg atacatagca
     1681 ttcgccgtcc tgctttttct gcttttcaat catcgaattg aagccgccta ttgtatcatt
     1741 cacaagtttg aacattgaac cgcttcggtc aataatgaat acaagttccg ttatgttgtt
     1801 tttcttttct ttcatgctga ttacctccaa aaaaaataat gcggtcgttc ggtctttcaa
     1861 ccttacaacc gcattataat catttttggg gtcatcatgg tcgcttgcca tgcgacactt
     1921 tttaagctcc gagcaaagct tggtcaaaag caaacagcac ttcgtttatt tcaaaaatat
     1981 tgtaattgcc gttcactata aaatattcga ttattatatc gaatttgttg ctgtgggaaa
     2041 gtgcaaagcc cgctttcata agaagctcct tggtttcgtc aagatcaagc tcaagnnnnn
     2101 nnnnnttata atgtatatcc gaacgaatct ttgaaaaaag ctttctgtcc acatttgcct
     2161 ttttgtagca ctcggaatcg gtcatgccct tttcgtcaat tttacgaaga agcatttgcg
     2221 aaaagctctc gtcaatctgc ttcaaggcgt cgtcaagact gtccaatgac tccttcgacg
     2281 ccgtatcgga cacagccatc ggcggcatcc tcttaaggcg tattgattcc atagcagcat
     2341 gcttttcaac atagcgctcg tctatatatt ccctgatatc cgaaacaagc ttttggctga
     2401 tgttaaagct cttttgatcg aaaacgacta tataaacggt tatatcgttt tcaaacaaaa
     2461 acgaactgat ttcgtccact gcgacctgca atgccttatc cttaggatat ccgtaaactc
     2521 ctgccgatat cagcggaaaa gcaacgctct cgcagtcatt tttctttgcc aacaaaagcg
     2581 attcacggta gcatgagcga agaagctttt cctcgccaga gtgtccgcct ttccaaacgg
     2641 gtccgacggt gtgaataata tacttgcacg ggagattata tccgcccgtg attttcgcct
     2701 cgcccgtctt gcagccgccg agcttcatac attcaagaag aagccctttt cccgccgcct
     2761 tatgtattgc cccgtcaact ccgccaccgc cgagaagcgt tgaattggct gcgttaacaa
     2821 tggcatcgca tttcattttt gttatatcat ttcttacaat ctgtaacggc atttaagctc
     2881 tccttagaaa ttttgttata tcggcattac cgatttttcg gagttaatcg catcaaat
//
LOCUS       DAWBSW010000232         2858 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5660_length_2858_cov_2.591152, whole genome shotgun sequence.
ACCESSION   DAWBSW010000232 DAWBSW010000000
VERSION     DAWBSW010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_2858_cov_2.591152"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2472..2481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          823 a          596 c          593 g          836 t
ORIGIN      
        1 tattcaaata ggcggtctcg gcgttattac cgtcgccgca tcttttgccc ttctttcagg
       61 gcgcaagatt tcccttatgc aacgcagcac gatgcaggaa gctattgcgg cgcccaagat
      121 cggcggtatc gttcgtctta caggcttcgt tttaaaagct acgcttattt tcgaattaag
      181 cggcgctgta ataatgatgc ctgttctttg caaggattat ggggtcaaag gagtttggct
      241 tgctgttttt cattcgatct cggctttttg caatgccgga tttgatatat tgggaacaga
      301 cgcttcgaaa tatgtttctc tgacgcagta cgtaaactgc acttctatta accttgccgt
      361 tatatttctt attgtaatcg gcggtatcgg atttttgaca tgggaggata tttatacaaa
      421 taagcttcac ttccggcgat accgtatgca aagcaaggtt atccttatta cttcggcgat
      481 acttataatt gttccggctt tatatttttt cttttttgat ttaaataacc ttccgataaa
      541 acaaagaatt ctttcttctc tttttcagtc ggttacaaca agaacagccg gattcaatac
      601 tgtagattta accgcgctgc aagaaccttc cattgcaaca atgatagttc ttatgattat
      661 cggaggttct ccgggttcaa cagccggcgg tattaaaacg acaaccgtcg cagttctcat
      721 tgccaattct ttggcaacgt tcggtcgcaa agattcctcc cattttttta aacgaagaat
      781 tgataatcag gttgtaaaaa acgcatcaac aatactgatc atgtatttgg ttctttcatt
      841 tacaggtgcc gtagccataa gcacggcaga gaaaatacct tttttaaaat gtctgtttga
      901 aacgtcttct gcaataggaa cagtcggatt aacacttggc ataacgccgg aattaggttt
      961 attctctcaa ctgattttaa tactcttaat gttctgcgga agagtcggag gcttgactct
     1021 gatatatgcc gcaatatcca atacaaagaa aaatatttca aaatacccct tagagcatat
     1081 caccgtagga taaaggagga tcatacatga agaacgtttt acttatcggt ctcggccgtt
     1141 tcggaagaca tatggctatg gatctcaacc gtcttgatca tcaggttatg gctgttgact
     1201 taaatgagga aagaatcaac gatattcttc cgtacgtcac aaatgcccag atcggcgaca
     1261 gcacaaacga acaatttatc agatcgctcg gtgtgaataa ctatgacctc tgcatcgtgg
     1321 ctataggcaa caattttcaa agctcacttg aaactacatc gttgctcaaa gagctcggtg
     1381 ctcaaaaagt catatcccgt gccgaacggg atgttcaggc gaaatttctt ctcagaaacg
     1441 gagccgataa cgtagtttat cccgaaaaac aggttgcgga atgggctgcc atacgattta
     1501 cggcagatca cattttggac ttcattgaag ttgacgattt gcacgcaatt tgtgaggttg
     1561 aagtgccaaa atcgtggtac ggaaaaaccg ttggtcaagt tgatgttcga cgcaaataca
     1621 atcttaatat tatcggcatc aaaaagaata ataatatcga catggctatc tcatctgaga
     1681 ctttactcaa taatgatgta actcttcttg ttctcggcga ctataaatct ttacaaaaat
     1741 gcttccaaat ctaaggtcat attatttgtc ttacggctct cctcttttcc gaatctattt
     1801 aagtcatata tatcaaaagg atctgtactt aatttacata tctcaattgt gagatacaac
     1861 caataaataa ctatacaatt ctatgaaagg cagaaattga gtgaaaatta ttgttgtcgg
     1921 aatcggcaag gtcggtttta ctgttgcaga tcagctttca aacgagcatc acgacgtaac
     1981 cattgtcgac accaatgaaa aagttcttaa tgcggttcta aataatcttg acgtaatcgc
     2041 cgtgcacggt aacggagctt ctcgcaaggt tctctccgag gcaggcgtcg aggaaagtga
     2101 tcttgttatc gccctcacag gaagcgatga aatcaacctg ctttgctgtc tgctcgcaac
     2161 aaaactcgga gcaaacggta caattgcacg tgtcagaaat cacgtatatt atgaggacgt
     2221 tcgtcttata aaagacagtc tcggtctttc aatggtaatc aatcccgaaa aagaagcggc
     2281 ggaggaaatt ctccgtattc tcaaataccc gtcggcaaag aatattgata cctttgcaaa
     2341 gggtaaaatc aatattgtaa gctttacagc tactaaaaac tgcactctct gcggtcaggc
     2401 ggtcaaagat tcattctcgg gacttaaagc gagagctctt gcctgtgccg ttgaacgcga
     2461 aaacaagatt tnnnnnnnnn ntcggtgatt ccgtaattga agaaaatgac gttgttgccg
     2521 tgctcgtttc gcccaacgaa accccgatat tttttaagga gatcggcttg cccgttaact
     2581 ctgtgaaaaa gccaatgatt atcggcggcg gcaaaatcgg tcattatctt gcaacggaaa
     2641 tgtgccgcct taatatggat acgacgattg ttgaaagcaa tccaaaaacc gctgaagagc
     2701 tcagctacct tcttccgaaa gcaaacatca tatgcggaga cggaacaaac cgcgctctgt
     2761 tgatggagga gggcttgcgt gacgcaagcg caatatgctg cctgacaggt tttgatgagg
     2821 aaaatcttat tctttctctt tttgccaaaa aggttgtt
//
LOCUS       DAWBSW010000233         2855 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5670_length_2855_cov_2.710357, whole genome shotgun sequence.
ACCESSION   DAWBSW010000233 DAWBSW010000000
VERSION     DAWBSW010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5670_length_2855_cov_2.710357"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          742 a          657 c          661 g          795 t
ORIGIN      
        1 gggcaggacg gtaagctttg aaaacacggt tatcgttatg acctccaacg ccggcagcag
       61 tcgctctgaa ggcgctctcg gctttggaaa aacgcagggc gatgtcaata aggaaaaggc
      121 gctaaaggca ctcagagagt ttctcagacc cgaatttctc ggcagagtcg atgaaattgt
      181 agtgttcaat gaccttgacg aagatgcgtt caaaaagatt gccgtgctaa tgcttgacga
      241 gttcaaggag ccgctcaggg ataagggcat aacgttcacc tataccgatg aagccgttgc
      301 agagcttgca aaggaatctg tcggcggaac gaggggagcg agagacctca gaaataatat
      361 tcgccgcaag gtcgaggata agattgccga gaagatcatc gacaatatcg acactcccgt
      421 aacatcaatc acggttgacg ttgagaacgg cgacatagtt cttaaataaa acagtacaag
      481 cctgccttga ttgacaaagc aggcttgatt ttttcggaag aatggatcaa tgcaattgct
      541 atttcgtgaa atctttgaac aaaattaaaa aatactggat ttttattata aagatgatat
      601 aatatttttg tgtaaaaata ccggcttttc cgcattcgtc ttttttgtga atttgggttt
      661 gtgtcgcagc aatcaagccg tgtatttttt gtgcgtggct tcggctgcgc actttttatt
      721 ttatggggga atttacaaat gagcttttcc gaaaacttta agaagcaaga aaaaacagcg
      781 gacttacgca gcaacagctt gcagatatgc ttgaaatgga tcgttcgtct gtttcaaaat
      841 atgaaaacgg aataggactg ccgcttgcga atacgctgca aaaaatatgc gaaattctcg
      901 gcgttacgat ggacagtctc tttaagtaaa accaaaacct ccgcctgtag gcttacacag
      961 acggaggttt ttatattgtt ttaattaaag catcgagcag ccaaagctgt tgatgagtgc
     1021 gtcaatgcgc tcacccttct tgcaaagagg acattccata gctgaatggc tctcatattc
     1081 aggcaggtcg tgaggatcga ataccgatac aacggggaag ttttcacact ctttcattgt
     1141 tgcgaaaata gaagcgattc cgaccggtgt acccgcataa taacggatgg cttcaaatgc
     1201 gttctgagcc gtgctgccgg taacgaccga agcggcaagg ataagaacct gcttgccctg
     1261 aatcatctga acaatgttgt cacggaacat gagctggttg cttgccgtgt actcaggggc
     1321 aagaatgtta atcgctctgt ttgcgttgat gcttgcaagg tcattcttcg tcagctcgct
     1381 tgcaagacat gtaccgataa cctctgtgcc gtcaaggcaa agaattgtgt cgatctccgt
     1441 atggagcttg tagttcgggg caattgcttt tgcaactgcc ttcgcctcgg aaagacatga
     1501 tttctgcgag gtgatgtcta tataataatt caagtggctt cttcctgcca caaaatgacc
     1561 cttgtagccg ctgagataga ggttcggggt ctttgttcca atcttaaact tttcacttaa
     1621 tgccataatg cttctcctct ctctgtcggc cgaattctca gccgcttata tggtactttc
     1681 tattttatca cgccgcagaa ataaatgcaa tatttacggc agacaaatat acaaaattta
     1741 tttaatgttt aatgttgcaa gtccgccttc ggcggtttct ctgtagcgtc cggggatatc
     1801 cttgcccgtc attctcatga cctcgatgac ctcatcgacg gaaatctttc ttgtgtcgct
     1861 caaaaagctt gcgaggctga cggcatttat tgccctcatt gccgcaacgg cattgcgctc
     1921 aatgcacgga atctgaacca gtccgcaaat cggatcacag gtcagtccga ggtggtgttc
     1981 aattgcaatt tcagcggcat actctatttt atctgtttca agagagaaaa gctcgccgag
     2041 tgcggcagaa gccattgcac acgccgttcc tatctccgcc tgacagccgc attcactgcc
     2101 cgaaatcgaa gcgttggtct ttaccagatt accgattatt ccgcccgttt caagtgcacg
     2161 gactatctca aggtcggtat aacccctttt catctgctga taatacaaca ccgacggcat
     2221 aacccctgcc gagccgcagg tcggggctgt tacgatgacc tctccgtccg cattctgctc
     2281 gcttacggcg aatgcatagg cggaaacaac gcggttttcc ttggtttgag cgctctcgtc
     2341 gatatgctga tgagaataga gttttttcgc ctttctcttg acgttcagtc cgcccgggag
     2401 aactccctcg gtttcaagac cgatatggat actcaacttc atctgattcc agattttcat
     2461 gagataatct ctaatctcga ttccctcaac ctcctcgaca tactgccaaa gcttcatgtg
     2521 attttttctg caatgctcgg caatctcgga aaaggttgac agcccgtaaa ttttcggcgg
     2581 atcgacattt ttgtaaccga caatttcaat ttttccgcct ccgacgctga gaacacggat
     2641 tcttttaatt tcaacgccgt ccttataggc gatcaggtcc attgtgttgg gatgcggaag
     2701 ctcaagatct tcattgttga aaataacatc gcaaggggta gggtcaaatg tgactttgat
     2761 aaccttgtcc gtgccgtgac cgactcccgt ttttgagagt gagccataga gaatcgcttc
     2821 aaaacggtcg gctttcggat tgtgctcttt aaaaa
//
LOCUS       DAWBSW010000234         2850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5679_length_2850_cov_2.495886, whole genome shotgun sequence.
ACCESSION   DAWBSW010000234 DAWBSW010000000
VERSION     DAWBSW010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5679_length_2850_cov_2.495886"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          749 a          572 c          693 g          836 t
ORIGIN      
        1 atttgccttt ttgaagccta tagattttcg ggcgtagcgg tcgcaacgct gtgctattat
       61 atggcgcctg taattgttgt tgcggcatcg ccttttattc tcaaggaacg tctgacggcc
      121 gtaaagggct taagtgttgc tgttgcggtt atcggagcgg tgctcatatc gggaattgtt
      181 acgggcacaa caaaaagctt caagggaatt cttttcgggc ttgccgccgc tgctttatac
      241 agcacggtta ttgttataaa caagttcgtt cgagagcttt cgcccgttga aacgactttc
      301 gttcagcttt cggtcgccgc agtcacgatg attccgtacg tcctgctgac cgaggatgtg
      361 acaggcttca gctttgatac gaagtcggtt atacttacct tgattatcgg cttgtttcac
      421 accggatttg cttatgtgat ttatttttcg tccgtgcaga aaatccctgc acagactacc
      481 gctgttttca gctacataga ccccgtaacg gcgattatca tttccgccgc cttcctcgga
      541 gaaagactgg atgccgttca gatagtcggc acggtcctta tccttgccgc aacgcttttc
      601 aacgagcttg ccccgatgat aagggagagg ctaaagaagg gctaaaggta tatgagctca
      661 tcctccggcg gagctgtcac ggcttgtccg tgactgagca tgcaagtcgg taaataaatc
      721 aacaaccaca tcccgaataa tttcatcacg aaaggaatgt tctttatgaa agaccttata
      781 attatcggct cgggcggtct cggacgagag agcgtatgga cggctgagag aattaacgct
      841 gtcaatcccg aatggaatat tctcggattt attgacgata atattcaggt acagggtaag
      901 cttatcgacg gatacagggt catcggaacg actgccgcgg cggttaaata tccggatgcg
      961 tactatgttt gcgctgtcgg aagtgccaag ataagaaaca gaatcataaa aaaaataaag
     1021 agcattgccg atgtgaggtt tgcaacgctt attgaccctg ccgccgtgtt cggcaaggac
     1081 agagtgcaga taggcgaggg ctgtattatt tgcgccaata cttatattac gcttgatatc
     1141 aaaatcggca gtcatgttta tatcggcgca aattcaacgg taggccatga ttcgagaatt
     1201 gacgattttg ttacagtcta tcccggagca aatgtttccg gaagcacgta tctcggttcc
     1261 gggtgtgaaa taggaacagg gtcaaaagtt attcaaggtc tttcgatagg aatgaactcc
     1321 atcatcggtg caggtgcggt cgttataaag aatattccgc aggattgtac ggctgtcgga
     1381 gttccggcta aaatcataaa gatgaatcga aatatataaa aaaggacggt atcaaaactc
     1441 atcagttaag ataccgtcgt tattatttat tgtgaaactc agaagccgat aatgtggctt
     1501 ataatgtcgc gaataatttt aagcaggttg agaagaacgg tcttgaatcc cgcaaagaag
     1561 gaattactgc ttgatccctt gtcgcagctg aatgtcattt caaccgttaa tgtaccgtcc
     1621 tgaatacgaa cgcttgacgg attttgaccg ttgaccgtta ccgtgagaga atcgtcaagc
     1681 ttgtaaccgt ccttaggtgc tattgagatt tttgccgtgt attctctgcc gtcctcgtag
     1741 acatagtccg aatccgcggc cgaaacaatt ccgtttgtct tggtatcata ccatgtgaaa
     1801 gccgttacgg tgacgttttc gctgcttgca acgtagtcta tgctcggctt ctgaccggca
     1861 atcggagtgt tgattatgat gtcaatattg cctgcgataa tgtaaacggg tttgtcgtca
     1921 gccgatacgg cgacgctcat tataccgaaa acggttatga cggcaatgag aattgccaaa
     1981 atcttttttg ttgttttcat actgattctc ctttattttt ttgacggcaa ataagccttg
     2041 accgtctgta atttatttta ttataacata aaaaattttt ttgtctatat acagtatcaa
     2101 aaaatgtcga ttttgattca gtgcgtcttg atataaatat gtatttatga tataatcgag
     2161 cggtaatttg taagaaggtg agtaaatgtt aaaggaaaga gcatcgggag ttttgatgca
     2221 tatttcttct cttccgtcac ggtacggaat aggcgttttt gatgataatt gcaggcattt
     2281 tgccgacagg ctcagtcaga tgaatttcgg ctattggcag gtgttgccgt tcaatccggt
     2341 tgacgggtcg aattcgccgt attgttcctc atccgctttc gcaggcaatt ttatgtttat
     2401 tgaccctgaa aagctttatt ccgacggact ttgtacaaaa gaggagtttg aaagtaatat
     2461 ttacggcgga agtccgtata cggcggacta tgaatttgcc gcagagaaaa gactcgccct
     2521 tttaaaagag gcttttctgc gtatcactcc agagacggcg cagaatatca agtctttcga
     2581 aaaggaaaat cagtggctta cggatttttc actttatatg gcactcaaag aagcaaacgg
     2641 catgaaaccg tggtggcaat gggagcctga gcaggcgagg tatgagattt tcatgcagaa
     2701 gcacaggtac gattttgagg agcgttcggc attctggcgc ttcgttcagt atattttcta
     2761 ttcgcagtgg tgtgacgtga agaaatatat caattcaaaa ggaattgccg taatcggaga
     2821 tatgcctatt tatgtttcat tggacagcgc
//
LOCUS       DAWBSW010000235         2800 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5802_length_2800_cov_2.456102, whole genome shotgun sequence.
ACCESSION   DAWBSW010000235 DAWBSW010000000
VERSION     DAWBSW010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_2800_cov_2.456102"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          795 a          626 c          550 g          829 t
ORIGIN      
        1 ttatgcttat gacggttgat cgcgtcgtcg aaaacaagca taaatgcagg cagaattatt
       61 acaaccgaaa gcacgccgat aatgacgccc tttgccataa caattccgat attaagaccg
      121 agcgtaagct gcatgaagca aagcgcaagg aagccgaaaa gcgtcgttag cgagctgccc
      181 gagagcgaaa taaacgaacc gtgcaaagcc ctcgacattg cctcttcttt cgtccttgaa
      241 tacctgcgtt cctcgttata tctgttgacg aagaatatcg aatagtcaac cgtaacaccg
      301 agttggagta tcgctgcgat ggactgggtg atataagaaa cgcccatgaa aaagtttgtg
      361 cccatattat acataaccgc tattccgatg actccgacga gaataaacgg catgacatat
      421 gattccatgg tgatcatcat tgtgattatt gcaagagcaa cggcaatgag aatataaatc
      481 ggcgtttcac tgtccgtcat ttcctttgtg tcggagttca tggcagaaat accgctcaaa
      541 tagctgtttt cgggaagaat ctttttaatc tgcttgaccg attttataat atcctttgac
      601 gaagcgtcgt cggcatagct gacaaacatc attgtgtact cgccgtcact gctgtaaaaa
      661 acatttttca cgtcatcggg caggatatcg ccgggaacgc ttgtgtccat aatattatcc
      721 gcccaaagaa catttgaaac attctttatc tcggaaatat tatttttaag cttcatcact
      781 gtattaggtg aaatttttcc gccgttatcg tctaccgttt taacaagcac aatagtcatt
      841 gagctgccgc caaaatcctc ttcaagaata ttttcaccct tgacagaatc gagccttgac
      901 ggaagatagc tcaaaatatc gtagttaacg tttgtaaaaa tgtagcttat aagtgacgga
      961 ataagcaaca aaagcgatat taaaacgatc aatttcggtt tattgacaac cagatcggat
     1021 atctttttca tttttaattc tcctgtttaa aagccaaata gattaaatat ggcaagaata
     1081 tagacaatta gtcctactac tattacgccg ctgagggaaa gaataacagc ctttaccttt
     1141 tcgtactcgt ccgcgctgaa aatataacgg agaaccgagc tgaatttatt tccgaaaaga
     1201 tcaaaatagc cgattgtata tgtctcaaag cttgtcggat cgttttcgtc aataacaaag
     1261 accctcgtgc ctcttgaaat catgttgccg tagcttctga atgaagcgcc gggggtttga
     1321 atgatattca ctccgtcgat ctgaccggta aagcaattca tatggtcatg accgaaaaca
     1381 agaccgcaga cgtcgccgca cttttttatt gccgtgagct gaccgtaatc gggttcgtca
     1441 ttgccctcga tttcaaacgc aaagcccttt gccttggttg aatcaagctt aacgtattcg
     1501 tcctcgccta cctttattgc tcccttgtcg tctgcattgc actttttgaa gaactgtgca
     1561 gtctctgcaa taggtatatg ctgaaacatc agggacataa cgggaccgtt gttttcaaga
     1621 cgaagctgac ggctttttct ctcgtaccat tcaatcgttt ccggctgaac tctgccgaat
     1681 atcggtctgc cgttctcgtc cgagcctgcc gaatcaagca tccaaatgtt gtatttcatc
     1741 tcgtcgccgg tcgatgaata gacgggaata ttataggtat cgcattcaac gctgttatca
     1801 tccatgttat agggcacgca gaagttatat tcaccgtaaa tctccgcctg ctccttcttg
     1861 gagataatgt tcatatcgtc atgatttccg tagatgaaag cgaaaggtat ttttcttctt
     1921 tcaatgggat cgagcagcag tccgagagct ttcctcattc ttttaagtac agcaggctta
     1981 gttgttgcgc tctttttctt tccgataagc gcatcgtcaa tatgattgcc gagaatgttg
     2041 tcgcccgtca agacgatcag atccggctta accttgtcat aggtcttgtc cagcattttg
     2101 aacatggctt ttctcggtat atgcatatcc tgagcatcac tgacttgaag aattgtaaat
     2161 tttccgtctt tattgaattt aagcttttct ttatacatta cagaaactcc attttgaaat
     2221 tatataaatt tattttatca taacattaag ctgtttgcaa gaaaaaagcc gtcatttaga
     2281 cggcaaatga gtagatttta caattatgta atatcattat ccttttttat tccgaaaaag
     2341 agtctgagaa ttccgctcag gaattttggc ttgtcgatca tgataacttt cataaaaacc
     2401 ctccgttatt tgctgatgat gaatccgaga ataatgacag cacaggaaag cacagccaca
     2461 atgaaagatt tcggacatat catagcaacg atcattccga gcccgaaagc cgaaaaataa
     2521 tagccgaact tacagccgca ccgcctgcct cttctcaaaa aaacaccacc acagcagttt
     2581 tgcaatcgaa aaaccggttg cttattacat actatggcgg tttagatttt ttgttacagt
     2641 tgccaatcct tgatgtcctt gacctcattg tacattgtca catagctttc gtgtcgtgtt
     2701 cgctcgacat caccgttttt tacagcctca accagactgc agcccttgtc attaacgtgg
     2761 gcgcaggtag aaaacctgca cgagccgata tattcggcaa
//
LOCUS       DAWBSW010000236         2796 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5817_length_2796_cov_2.024808, whole genome shotgun sequence.
ACCESSION   DAWBSW010000236 DAWBSW010000000
VERSION     DAWBSW010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5817_length_2796_cov_2.024808"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1853..1862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2219..2286
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          769 a          572 c          600 g          777 t
ORIGIN      
        1 gttctgcggc tatgcttacc tcttcgggac aggctgcctc attctatgca gtgttcaata
       61 tcgcaaactg cggcgatcat gttgtcgctt cttccatgat ttacggcgga acatataatc
      121 tctttgcagt tacgatgaag aaaatgggca ttgacttcac ttttgttgag ccggactgct
      181 cggatgagga gcttgaagcc gcattcaagc cgaataccaa gcttgttttc ggtgaaacta
      241 tcgcaaaccc tgctttgact gttttcgata ttgaaagatt tgcaaatgcc gctcatgcgc
      301 acggtgttcc tcttatagtt gacaatacgt ttgcaactcc tattaactgc cgcccgtttg
      361 agtggggagc ggatatcgtc actcattcaa cgacaaagta tatggacggc cacggcgcag
      421 gcgtcggcgg ttgtatagtc gattcgggca gatttgactg gatggctcat gccgataaat
      481 tccccggact ttgcactccc gatgacagct accacggcat tgtttatgcc gagcgtttcg
      541 gaaaagaagg cgcttttatt acaaaagcga ccgctcagct catgcgtgac ttcggctcta
      601 ttcagtcacc tcagaacgct tttattctca atctcggact ggaaagcctt catgtaagaa
      661 tggcaaggca ctgcgaaaac ggtcttgcgg tcgcaaagtt ccttgcttcc catgataaaa
      721 tcgagtgggt taattacccg tcgctcgagg gcaacaagta ttatgagctt gctttgaaat
      781 atcttccaaa cggctcatgc ggagttgtca gcttcggaat aaagggcgga agaaaggccg
      841 ccgaaaaatt tatgaaaaat ctcaaggttg ccgcgattga aacgcatgtt gccgatgcaa
      901 gaacatgttg cctgcatccg gcgagcgcaa ctcaccgcca gatgaacgat gctgagcttg
      961 ccgctgcggg agtacccgca aaccttgttc gcttctcatg cggacttgaa aattcccaag
     1021 atcttattga ggatattgca caggcgcttg agaaaatctg attaaagagt gatatctaat
     1081 gccaattaaa ataccgaacg gattgcctgc cgtcaaggtt ctccacgatg aaaatatatt
     1141 tgtaatgacg gaaaaaagag cagttaagca ggatatcaga ccgttgaaaa ttctgcttct
     1201 taatcttatg ccgaaaaaaa ttgaaacaga aacccagttt gcgagactgc ttggcaatac
     1261 tccgcttcag gttgaaatgg aaatgatgta tacaacctct cacaagtcga aaaacacctc
     1321 tgagggacat ctgttagcct tttacaaaac ctttgacgag attaaacaca ataaatatga
     1381 cggcatgatt attaccggtg ctcctgttga aaatatggag tttgaagagg ttgaatactg
     1441 ggacgagctt tgcaaaatca tggaatggag caaaacccat gtcacaagca cctttcacat
     1501 ttgctggggc gctcaagcgg ggctctatta tcatttcggg attccgaaaa tccatcttcc
     1561 gcagaagtat ttcggcgttt atccgcataa ggtaaactat aaaaagtcga ttcttttccg
     1621 tggttttgac gagacattta tggtgccgca gtcaagacat acaacggttt tgaccgagga
     1681 cataaaaaag gtcaaagaat tgaaaattct tgcgtcgtca tcggaaacgg gagtttatgc
     1741 tatctccacg aacaagggca agcagatttt cataaccgga cattccgaat atgaacgcga
     1801 cactttgaaa aacgagtatc tcagggataa ggaaaagggg cttgatatcg ccnnnnnnnn
     1861 nnttccgaca aattattttc cgaatgacga cccgtccaag gaacctattg tctcatggag
     1921 agctcatgcg aatttgcttt tctccaactg gctgaactat ttcgtatatc agacaacacc
     1981 gtatgatatc gatatgattg aatgataaaa atataaagca agtaaatttt taacatcttg
     2041 ctaacctgtc aatttcctta atatttcttt attctaaaaa ctgttgacgg ataaggtgct
     2101 tgtatgatat gatacagaag tcagcaccat gttgatagta tttaaaacaa aggagtcttg
     2161 gatggaaaag aaacacgagt taaaattctt tgcaaagcct gcaaagggaa aaatgaaann
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnggca tgagctgccg attgcaaact gggcgttttt tatcagcgta acgattttct
     2341 ttatgcagga agaacttacc tacaaagctc gtttttggca cacgcttgtc ggaggagccg
     2401 tcgggcttct tcttgctgca ggtcttgttt gggttaatat ttatcttaaa acacgcggtg
     2461 ttccgtcgct tgtttcgata atgatacctc tgacgatttg tatcgcagcg ttgattttgc
     2521 ttcaccctta tcttccggtt ttcttcaaca atgtcggatt ttgctatttt ataatctcgc
     2581 ttattgatgc aaagtcggca attgataagc tgccgattta tcttttttct ctgcttctcg
     2641 gaagcatctg ccttaacctc ggttgcagcg gattgcttgc tctttataag aagtcaatga
     2701 tgaaaaaggc atcggcaaag atttcttcgg aaaaagaata aattacttaa tatttttcac
     2761 gttttaattt gctgaaaaat ctgaatattt tcaacc
//
LOCUS       DAWBSW010000237         2753 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_5914_length_2753_cov_2.416976, whole genome shotgun sequence.
ACCESSION   DAWBSW010000237 DAWBSW010000000
VERSION     DAWBSW010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5914_length_2753_cov_2.416976"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1320..1329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          843 a          556 c          595 g          749 t
ORIGIN      
        1 ctctgccaac tgagctaatg aatcgcaatg cgtatttaac gcttgaatat aatacatcaa
       61 agaagataaa aagtcaagca tttttttgaa atattatgat ttattttatc ttttttctct
      121 gcaactttta taatgagaat tttgctattg atttattcct cctgtcgata taaaatataa
      181 ttatattata tcaacggagg aacgtaatga ccgaggacga gctgctttat gccgtgcttg
      241 acgataagat aagagaatgt gaaaacaaca actacctttc caatacgaat tttctcgaca
      301 tctatcagca gagcaaggct attcaatatc ttcaaaaatt tcatattaag catgtccttt
      361 acggcggctt tgaggaatcc gagcgcagaa tcgtaatctt tctccccgac tatgccgatg
      421 acgaatttct ctttgagtgt gaagatttgc ccattgttcc gctgagaatt gacaaggaca
      481 atttttctac gctttcgcac cgtgactatc tcggggcgat aatgggtctc ggaataaaaa
      541 gagaaatgct cggagatatt ctcattgacg ctaagggctg cacgacggcg gcggtcaaaa
      601 gcgttgcaaa gtatctttgt gaaaatctca tatccgttgg cagaggaacc gttcatacac
      661 acattcttga aagcttcaat acagttaaaa ccgaagaaaa atttgagact aagcgctgct
      721 tcgtatcatc tatgcgagcc gattcggtag tatcttcatg cttcaacctt tcacgtagcg
      781 aagccgttcg cagaatcacc gcaggcgagg tttatataaa cggaatacag attacaaaaa
      841 ttgattataa aatccctttc gacgccaagg ttgtcgtaag aggtaagggc aaggttctca
      901 ttaccgagga ctgcggaatt actaaaaaag ggcgacagac cttcacaata aaaaaatata
      961 tctgaggaga atttatatgt taaagttcgg acataggggc ttcagcggaa aatatcccga
     1021 gaacacaatg ctcgcattca aaaaagctgt tgaagcaggc gcagacggaa ttgagcttga
     1081 cgttcatttt tccaaggacg gcgagcttgt tattatccat gatgaaaaaa tcgacagaac
     1141 gtgcgattct acgggctttg tcagagacta cactcttgcc gagcttcagt cttttgacgc
     1201 ttctgcgggc tttaagggcg tttacggcgt aaacagaatt cctaccctgc acgaatactt
     1261 tgaatttgtc aagccgattg acggttttat gacaaacatc gagcttaaaa cgggtgtttn
     1321 nnnnnnnnna accattacag cgtcatgcgt tttaaagcaa ttaacccaaa tatcaagtgc
     1381 ggttttctcg aagaaagctg gattctcgaa ttcggcaagt acactaaaaa tcacggcatc
     1441 gaatgtattc atccgatgca ttggacattg acggaggaaa ccgtcggtga aatgaaagaa
     1501 aacggaatcg aaataaacac atggacagtc aacgatgagg acagcgttgc aaggctttat
     1561 aagctcggag ttgactccgt aatcggaaat tatcccgata tggcgaaaaa agtgatagat
     1621 tcgatgaaat aatacttgat tttatcatac attcagtgta ttatgataaa ggcttagaaa
     1681 gctaaaaaat atcagaggtg ttaaaatgtt tacaggtaaa gcagaagaat tcagaggcgt
     1741 atatgccctt cttctcactc cgtttaatga agacagatcc atcaactatg atacatatgc
     1801 acagtacgtt gagttccagg cagcaagagg tgcgcatcac cttttctcgg tatgcggcag
     1861 ctcggaaatg acatgcctca ctctcgacga aagaattaag tgcgccgaga cagccgttaa
     1921 acattcaaac ggcgttgaaa tcgttgcaac agccaacctt gagccgacct ggaaagagca
     1981 gatcgaagag gttaaacgca tggagcaaac cggcgttgac ggtcttgttt ttgttacgag
     2041 ggattacggt aactgcgata aaagaatagt tgaaaatctt tccgagcttg caggatatac
     2101 aaagcttccg attatcctct acgagttccc gggatttgac cattgccata tgtcaggcaa
     2161 ggcatacggc gagcttgtta aaacaggcag attccacgga ataaaggata ccacttgttc
     2221 tatggacggt attaaggaca aaattgccgt tcaaggcgat tcgtcggttc ttcaggctaa
     2281 tattcccctt cttcttgacg cttatgatgc aggcgcacga ggcgtttgtg caactcctac
     2341 ctcatgcggc gtaaatcttt tccgcaaaat gtatgatgag tattttgtag agaaggatat
     2401 gaagaaggca agagaaacct tcaacgagat catactcctt gactgtgcta tcgacagcgg
     2461 cttcaatgca agcgcaaagt tccttgttca gctccagggt attcctttca caacggttac
     2521 aagagcaggc gcaacgctta acgcgccgag aatcagaagc ctccagtcct tctatgagta
     2581 cgcaaaggca cacgagcttc tttaatcaga ataaaataat cgctcacaag gctttcgggc
     2641 tttgtgagct ttttatatat aaatgtacca ttttgaaaaa gcatccactt tctctgtgat
     2701 taaagtatat gagcttcggc tttttcttgt agtttgcgaa tatccgtata aat
//
LOCUS       DAWBSW010000238         2703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6052_length_2703_cov_2.565710, whole genome shotgun sequence.
ACCESSION   DAWBSW010000238 DAWBSW010000000
VERSION     DAWBSW010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6052_length_2703_cov_2.565710"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          744 a          617 c          488 g          854 t
ORIGIN      
        1 gctgaaatcc ttaatcattc cgaccgaata tctgagtata tccaatgcat cgaccggatt
       61 gattttttca tctttgttga catccgcacg cttagcggct ctgccctgaa gctgaataac
      121 attgaccgaa tgttgcaaga tgcgcaatgc gtcgactgaa ttgataactc cgtcggcatt
      181 gacatcgccg attgctgcat tgctgttttg aacaattgca aatctgccga ggcggtttgt
      241 tttaaacgtt gcatatccgg caaatacggt ggcgtcaact ttgacagctg ttccgtcgct
      301 cgtgtcaagg ctgcatacac tgatagcaaa acggtcaaaa ttatcgggaa ccggaatgct
      361 tacagtaacc gaaccgtcag gctgaaccac agcattccct ttcttaattg aaacatcaaa
      421 catcttcgct ttgacttcgc cgaagctcat gtttacaagc ttgagtgaat ttacgtcatt
      481 tacttcactg acttcaagct ttaaattttt gtcgggccga tttttgaatt caattttaac
      541 acccgtcttg gcgtctgtta gtattttatc cccggcggaa gaagcttcga gaattttttc
      601 gtaataatcc tcatttcttt tcatttccca cgtccaaacc gagccgtcgg ctcttgtgaa
      661 aagtacatag ttgtggcact ctgtaaagtc aacaacattt gtcataattt ttacagggtt
      721 ttccccttct ctgattcccc agcacagagt ataaatagct ccgtatgttg aagcatataa
      781 gttgttatct ttgtcaagat agcagatgtc gccgtttttc tttacattcg tcaatacggg
      841 agagcccaaa tcatacttgc cgttgctgcg agtcacatta ccgagcatat acagacttcc
      901 gtttttactg aggaagccgc caagctctaa attaaaacgc tcaatattat ctgcgacttt
      961 ttcggttgtg ccttccgaag tgatctccca aaggacgccg ctctcatcaa tataaaaata
     1021 gctcgaataa taagacaaaa catcttttgc aaatttgccg ctgcaaatct ttatcggctt
     1081 ataatcatcc atgggaaaag aataatagat acctgtgccg acgtaatatg tgccgtcttt
     1141 tttattgtag atacattcac cgtgtgttct tgtcttcaca attgatatta catcggacga
     1201 gaccaaaacg tttttaaata aatcgtcccc tacctgataa gaaatagaat cataacagcg
     1261 atagagttca ttatcttttg tcaggtagca cataccgtta atatctttaa cattttttgc
     1321 aaccaactta aaatcgttaa gctcccaaca gttaccttta ttatcaagtg caagacccga
     1381 aacggaaatt tcattgtttg gggtaatctt tacaaaaccc gtgccgattt ttttaggaac
     1441 gctcgatttg cccgcaatag tccaaagggt gccgtcctcc gtaagataat atgtaccgaa
     1501 atcggggttt cttgcaggag tagaggtgtt cataataacc tttgaattga atttgatcat
     1561 ctgcttgtcg gtatagaatt tgccgtcggc attatatgaa ttatttgtaa gcaattcggg
     1621 aatgttattc gttttctcac ccatcggctt gtcggcagat acgacggtta cctcaaagct
     1681 ttttgacaag cccgcgcaaa aagcggttat agtggccact cccgctttgc ctgtacaaat
     1741 aacatccttg tactcttctt ttgatacctt agcaatttcg ggatcagagg acgaatagct
     1801 tgtatcctct ctgtaatcct tcatagcgat tctgctgtaa atataaatgt ttcttcttgc
     1861 attttcaagc atttccaagc tgcgatagct ctgcaaggca aaaacatctt ttaccggtgc
     1921 agtccagcca tcgcccgaaa gcttcaaatc cggtcctgca tattcgagac ctgatacatc
     1981 acttacgccg tcgaagccaa aagctccgtt taagcttctc atttcagtaa cagagatcat
     2041 tccgtcaccg tttttatccg ttccggaatc aataagcttt tcaaaaagtc ccgaatcgct
     2101 gaaattgatg tactgctcgg attgacttga aacgcacaca gtcgagttga cagcgtattc
     2161 accgtatttg attttgactg tctgagcacc tgcctgatct gctttgtagc cgctcgcaga
     2221 ataacctgtt accttctccc actccgaatt gctgttcatc acatagactt caatagccga
     2281 agaaagcgga gttccgagac tgataatcgg gtttacaaat ttaattttta ttttttcgac
     2341 aggcttatta tcattttctt cttccgataa atcatcggca taggtataca ttgatttcgg
     2401 taaatcctga accgcaaaag cacccatcgg cagtgccgac ataatcatta gcactgctaa
     2461 aaccaaagaa aaaaatttct ttttcattta tttttcctcc atagaacaat ttatttatac
     2521 tttcattttt tgtaaaattt gagatctacc ctctctgcga tacctcacat gagagaatag
     2581 ttttcttagt ttctccgcta acacctcccg gttttgaatg tccttagtct ttcaaaacct
     2641 gtgaacaact tcttgaaaaa tttatatgtt gaaaagaaaa agcatatcaa tacccgacaa
     2701 agg
//
LOCUS       DAWBSW010000239         2654 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6201_length_2654_cov_1.835321, whole genome shotgun sequence.
ACCESSION   DAWBSW010000239 DAWBSW010000000
VERSION     DAWBSW010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_2654_cov_1.835321"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          797 a          490 c          580 g          787 t
ORIGIN      
        1 ttatacaggc gaacacgtcg tttattgttc ttgatcccaa gggtgagctt ctccgtgata
       61 cgggaaatat gttaaaagat aaaggctatg atatcaaggt tcttgatttg attgatatgg
      121 aaaagagcga ctgctataac ccttttgtgt atattcataa cgataacgat attcaaagac
      181 ttgttacaaa cctctttaag aacacaacac cgaaaggaag tcagagcaca gacccgttct
      241 gggatcaggc ggcaagtatg ctgttgaaag cgcttatttc atttcttcat tatgaagctc
      301 cgccggagga acaaaacttc tcgatggttc ttgaacttat tcgagccgga gatatccccg
      361 aggaacagac agactttgag ataaccagtc cgcttgacga tatatttgca cgtcttgaag
      421 cacagaaacc cgatcatatt gctcttaagt attaccgttc atatcacagc ggtacagcga
      481 aaacattgaa gtcaattcag attgcattgc ttgcaagact tgagaaattt aatcttgact
      541 cattcgcaaa tatgacttgc gcagacgaat tggaacttga tcaaataggc gaaagaaaga
      601 cagcggtatt tgctcttatt tccgaaaacg atacaagttt taattttatc gtcggaatgt
      661 tatacactca gctttttcaa cagctgtatt atcaagccga ttatgttcac ggaggcagac
      721 ttgattgtca tgtccatttc ataatggacg agtttgcaaa catagcgctc cccgatgatt
      781 ttgataaatt gctttcaact atgcgttccc gtgaaatatc ggtatcaatt attattcaga
      841 atatggcaca gttaaaagca ctttttaagg atagctggga aaacatcacg ggtaactgtg
      901 atacactctt tgaaaagcag tgggaatcta ttgttggcaa ctgcgatgaa tttctttatc
      961 tcggaggtaa tgagcaatca acgcataagt atgtgtcgga attgatgggc aaagaaacca
     1021 tagatacaaa cacttatgga cagtccaaag gcagaaacgg aagctattcc acaaattatc
     1081 agaccgcagg cagagatctg ctgactcccg atgaagtgcg aatgctcgac aacaagtatg
     1141 cacttctttt cattcgcggt gaaagacccg ttaaagatct gaaatatgat attttaaagc
     1201 acccgaatgt aaagtacaca accgacggtt cgggagaagc gtatgttcac ggattcaatg
     1261 attactcgat acttgaaatg aactgttttg attttgatga aattgaaaca gatgaattgg
     1321 aaaacgtaaa ttacgttatt tattcaaacg aagaaatcga aaaaatatta ttgcaactgg
     1381 aggaagaaaa gtgaaaaaag ttaataattc taaacatttg aaaggcaagg tcaggaaagg
     1441 ctttaaagcc tatgtttatg ctgttctctg tatcacaatg cttgtgattt ttagcgttac
     1501 ggcttttgcg gcaggtgatc ctgtcggtgt tgtaaacaat ctttcaacat tcatcttcgg
     1561 acttatcaga gccgtcggtc ttatactgct cggcttcggt attgttcagg tcggactttc
     1621 tttgaagtcg cacgatccat cacagagagc taacggattt cttaccgttg ccggcggtat
     1681 tgttattacc tttgcaaagg aaattcttga tttgattgtc aactgattac tgatgattac
     1741 ggtcggcagg caaattaacc ttgccgatcg tattaacttt aagggaggtc tttaatggaa
     1801 gaatcaagta attgggtagt gcaaaatctc cagaatgcac ttgccacttg gaacaataaa
     1861 ttaagtgaaa tatggcttct catcactcaa tcacccgaag agttcaagga cggtacaata
     1921 tggactgtga taaccaacat tcacggagca cttcaggcaa taggatattc gcttctggtt
     1981 ttattctttg ttgtcggagt tatgaaaacc tgcggcagct ttgcggaaat caaaagaccc
     2041 gaaatagctt taaagatgtt tattcgtttt gtgcttgcaa aagcctctat tgactacgga
     2101 cttgaactga tgacatcact ttttgaaatc gtgcagggca tcgtttcaaa aattatggag
     2161 acatcgggat tgtcaagtac aaccgaaacc gttttaccgg agtcaatcat aaaggcgatt
     2221 gaggaaagcg gattttggga aagcgtaccg ttatgggcag tgaccctcat aggcagctta
     2281 tttattaccg ttttatcgtt cattatgatt atgacagtat acggacgatt ttttaaaata
     2341 tatttatata ctgctattgc tccggtgccg ttatcgtcat ttgcaggaga gccgtcgtcc
     2401 tccgtaggaa agagttttgt taaatcctat gcggcggtat gcttagaggg cgcaattatt
     2461 gtgctcggtt gtataatctt ctcggtgttt gccgcttctc cgcctgttgt tgacgaatcg
     2521 gctgccgccg ttaatcaggt gtggagctat gtaggtgaat tgatattcaa tatgatcgta
     2581 cttgtcggca cggtaaaaat gagtgacaga gttgtcaaag aaatgatggg actgtaaaaa
     2641 acactttaca aatt
//
LOCUS       DAWBSW010000240         2634 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6261_length_2634_cov_2.162854, whole genome shotgun sequence.
ACCESSION   DAWBSW010000240 DAWBSW010000000
VERSION     DAWBSW010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6261_length_2634_cov_2.162854"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    235..244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          748 a          516 c          607 g          753 t
ORIGIN      
        1 gtcccgatgc aacggtttat gccgtcgaaa aatccgatat agcttttgaa tatttggata
       61 aaaatatcag tctcaacggt gtgaataatg ttaaggctgt caaaggcgat atgtttgata
      121 aagaacttct ttttgggata tctcccgatt tgatcctttc aaatccgccg tatatccgtt
      181 caaatgaaat tgacggattg caggaagaag ttaagagaga gcctgcaatg gcgcnnnnnn
      241 nnnnctttta tcgttcgctt gcaaaggatt ggattcatcg aatcaaaaaa agcggagcgc
      301 tcgcctgtga atgcggcgag gatcaaacac attatatttg tgatttgttt tccgaaagct
      361 ccgcggaggt caaagaatat aaggactaca gcggtttacc gcgaggtgtt acagttatta
      421 tatagttttg gagaataata tattatgtta cttgatttaa taaggggcgg caatgcgctg
      481 tcggtattta tgggtgtttg cgttagtgca tttgttgttt tttgcttaat gccgatacac
      541 gaatatgctc atgctttagt tgcgacaaag ctcggcgacc aaacggcgag actcagcggc
      601 agactgacgc tcaatcccat ggctcatatt gatatcttgg gtgccgtaat gattttgctt
      661 gtcggcttcg gttatgccaa gcccgttccc gtaaatgtac gcaacaccaa gatgaaaaac
      721 aaaaagcttg caatggctct tattgcactt gcaggaccgc tttcaaattt gattttcggg
      781 tttctttccg tgcttgttag atatgtgttt cttcttgccg tctcaaagcg cggcggtgaa
      841 atgacaacag cggtctttgc tttggatgct ttcttccagt attcggcgat aatcaatata
      901 aatctcgccg tgttcaattt aattcctatt ccgccgcttg acggttcgag aattcttttt
      961 gcgatacttc cgaacaagtt ttacttcgga attatgaaat atgaaagata tattatgatt
     1021 gcaatgtttc tcctgcttct tacaggagcg ctcacaactc cgctgtcgta tctttccaat
     1081 ttgatttaca acgcatttaa taaatttttc tatataattt tttaaaaagg tgaaatcatg
     1141 gaagcgatat catataagct tgacgtgttc gagggtcctt tggatctgct ccttcacttg
     1201 ataagcaagc ataaaattga tataaacgat attcctattc ttcttcttgt tgaacaatat
     1261 cttgactatg tacgtcagat gaaagaggag aatatggagg ttgccagcga gtttctcgaa
     1321 atggcggcaa gactgatata tattaagacc gtttcgctcc ttcccgtgca cgaggaagaa
     1381 gcagacgaat tgaaaaagga gcttcagggt gagctgctcg aatatcatga ctgtcagatc
     1441 atggcaaaaa aactcgccgc tcaggcagac ggtttcgatc aatactcgag agagcctgag
     1501 aaatttcctc cggacatgac ttatacacgg ctgcatcagc cgattgagct tttgaaggcg
     1561 tatattaacg ccgtaggcag aggaaaaaga aatctcccgc ctccgatcga ggctttttcg
     1621 ggtatcgtag cacacaaaat agttccgata agagaacgtg tcagttatat aatgggcagt
     1681 cttttcagaa agaaaagaca acgctttgca gcgttctttg aaaaggcgga atcgcgctcg
     1741 gagatggttg cgacatttct tgcaatactt tcacttgcaa aggacaaaaa aattaccttg
     1801 actcaaactg acgatgattt tgaggttgaa atgattgatt ccgatattaa cttttacgtg
     1861 caggacgact acacaaatga taatgaatga actacaggga gctgttgagg caatactttt
     1921 tgccgcaggc gaaccgcttg aaacggaacg aatctgtcag gctctcgaat gtgaaaaaga
     1981 acctgttgag gaagctctca gaggaattga agaagccttg aagtccgtaa acagcggaat
     2041 aagacttgtt aaaatggata atatgtatca gctttgcacc cgtcaggagt atgccgataa
     2101 agtcagagcc gtgcttgaaa ttaaaaagaa cactccgctc tcgtcggcgg catttgaggt
     2161 gcttgccgtt atcgcctata atcagccggt cacaaagtca tttattgagc aggtcagagg
     2221 tgttgactgc tcaggcgtta taggaacact tatagcgaag ggtcttatcg aggaaagagg
     2281 cagacttgag ctgccgggca gaccgcttat ttacggcact acgcccgaat ttctcaaatg
     2341 cttctgcgtg acttgccttg acgatttgcc cgatcttccc gaacatgagg atttgccgaa
     2401 gagcgaagaa atcaacggcc agcttgaaat taaccttgac gacggtgaaa taatcgaaaa
     2461 taaaatgctc acaggcgaac cggttaaagt agcagaagaa taaaaacgga ggtgattgaa
     2521 tgacagcatt atacataatt ctcggaatta tcgctttttt cgttatagtt ctgagcatac
     2581 ctgtcgttct tgatcttgaa tataccgacg cagtgcggtg taaggtttca tggc
//
LOCUS       DAWBSW010000241         2594 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6390_length_2594_cov_2.015360, whole genome shotgun sequence.
ACCESSION   DAWBSW010000241 DAWBSW010000000
VERSION     DAWBSW010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6390_length_2594_cov_2.015360"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1145..1154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1917..1926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          757 a          520 c          549 g          748 t
ORIGIN      
        1 attcagaggc tttggagctt ataaggcaga gcgaagaaaa atacaaaaat ctataataaa
       61 aagctgtttc gatttcgcaa gaaaaaggaa cagctttttt aaaataataa ggttaaaata
      121 cagcgcaaat ctaaaatctt accgtgatct ctccgctgga gagatttttt gtttcgccgt
      181 tttcgctgac gattaaattt gcattgtcgt caatttcgat tgcttttgca aaatgctcaa
      241 tgttgttttc gtaatatttt atttctttgc cgatgacgca ggattttttt ctgtaataat
      301 caagataggt tctgtcatca atgtcatcgg caaatgtgat cattttttcg gtgacttttg
      361 caacaaaaat atttctgtca atttcctcac cgagcgagcc tgctcttgtg ccgatatcgt
      421 cgggaaaatc cgttgtgctg atattgacac ctattcctat gcacagtccg tcaacctttc
      481 cgttttttac aatcgactga acaagaattc cgcagacctt tttgccgcta acgtaaatgt
      541 cgttcaccca cttgattttc gggtcaaggt ctgtcatttc ctcaatcgtt tttgcaaccg
      601 tgacagctac ggcagaggta acataaacaa cgtcattcaa attgccctta ggacgaataa
      661 taattgagaa atatacgccg cttttggcag gggaatagaa gcttttgccc tgtctgccct
      721 tgccgttcgt ttgttcctcg gcggcaacaa gaatgtttcc ttcaactgtt tctgcaagct
      781 tgatcgctct tgtattggtc gaatctgtca cgtcataggt tacaacggtc acgttcggca
      841 gcattgatga aattgtttcc tgatttagga tattttcacc ctctcggtga ttgtgtttcc
      901 actaaaataa tatcactcaa ttcatctttt tacaagcaca ttttttgttg cggggaatag
      961 aatgtaatat aatgatgaat gaggtgatcc gatggacaac agttgctttt attacggagc
     1021 aaacacggcg gacggggtgc actcgggaat ttttgagcct gagaaaattg aaaaaatact
     1081 agttgttaaa ggcggatcgc ccgagctgaa ggacaaaatt tttattgagc tttctgagga
     1141 gcttnnnnnn nnnngctttt ttcagaaaca atcccacaag agcttttaca tatttacgag
     1201 aaagaatacg ataaaaagat aaaacgcagc ggaagatttt tgtcggcggc ggacagcgta
     1261 aaaaaagacg caatgcgtat tgacgcaaag tctgtgaaca tcggaagtgt tgtaaactat
     1321 tcttcaaaac tatggaaaaa gctcggcgca gagatgcagg gaagcatcgg gacggagaca
     1381 agaaagttcg tttcctgcat aactcccgac ggtgcggagc tgaacatgaa agcctttgat
     1441 gcttgcgatc gtttggctgt aattgttgac aaaacgggag tggtttcaac gatgataata
     1501 gaccgactgc gtcgatacgc actcggctcg ggttatgatg ttgtgagctg tatatgctca
     1561 ctcgacggaa aaacggttga gcatattatt gtgcccgagc tgagcttcgg agctttttca
     1621 tcagagcatt atcatagaat tttgccaaaa aaagagcgca gagtttttgc ttcacgtttt
     1681 catacaacgg acagcgagct ttataaaaac agaataaatt tttcgctgaa agcttaccgt
     1741 tcgctgatga acgaagcctt tgagtcgctg ataggcgcac agacggagga aagaaaaata
     1801 aattatattt tcggcaacgt tgttgatgtt tacaatgcag ttacggaaat aatcgaaagc
     1861 atataaacag aaaaatccga ccattgaaat tatcaaaggt cggaaatttt tttattnnnn
     1921 nnnnnngaaa taaattgcag aagataatgt attgccgaaa tttgttcctt gccgtatttt
     1981 cttctcagaa gaattatgtt tgccgctccg aaaaatccgc tttgcacaat gaatgtgcag
     2041 gtgtaaataa tcattataat aatgatgaat gacagaaaac cggctgttgc cagggttagt
     2101 ccgaaaccga ccatgaagca tacggcgaaa ttcagaatat aaaacggaac ggttttaatt
     2161 ttcaacagaa cccaagcctt tgaaagctgc tccgtttgtg attgcatgaa cattttaacc
     2221 gccaaaacgg cacacatcca gccgataacc tgaaccgcta tgaagaaaag tacgcccaaa
     2281 gccgaagtga tatatgatat taccgtcagc ttttcgctcg taacgccttc aatgacaagc
     2341 aaagcaagcg gcagaacaag ctgaagatga agcatcacaa aaaatgcttt tatcaatttc
     2401 attttctttc cgcccctttc ggcttatgcc tgaaccttgt ggaaaatctt cttgcccttc
     2461 ttcacgatta ttgcgtttgc cttgaggtca tccgaggtga atgaaagtcc gatatcggtt
     2521 actttcttat cgtcaacgga cacgccgccc tgctggacaa gacggcggcc ctcacccttc
     2581 gacgggatca gctc
//
LOCUS       DAWBSW010000242         2492 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6742_length_2492_cov_2.305704, whole genome shotgun sequence.
ACCESSION   DAWBSW010000242 DAWBSW010000000
VERSION     DAWBSW010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_2492_cov_2.305704"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          815 a          470 c          619 g          588 t
ORIGIN      
        1 gtttattatg agttgcccgt tcttaaacga gctggcacgt gtcaatgttg atttcctccc
       61 ggctgacccc gacacatact cggtcgaaga agtgccgtcg gaaacgattc gaaaaaaata
      121 tcttgacggt tcgtcggaaa ggcagtttat gttcaccttt gcggcacggc tcagctacag
      181 cgatgaggtt cgcaacaaca ttgacaacag cggatttttt gaggactttg aggaatggtt
      241 ggaaaaatgc acggagaccg acaatctccc tgaattaaga gagggcttga cgcccttgaa
      301 aattgaagca atttcaagcg gctacctgtt tgatatatcg ggtgatttga aaaattctcg
      361 ctatcaaata cagtgccgct taatttatga taaggagtga ctttaatgga aaaagtaaaa
      421 tccagtaagt cggcggtgct gcttaatatc ggcacggcgg catcaccgga ctacaagcgt
      481 atcggtaagg gtgtaacgtc attgccgata agctataatc cgaaaaccac aacggaaaca
      541 tacgttgacg aggacaatgc gacaacctcg gttgacagct atgagatttc ttcggatatc
      601 gaacagactg caatcaagga cgatccgatt tttgattatg tcgacggaat aagacgagga
      661 ttgaaaacgg gttcggattg tgaaacaacc gcagttttgg ttgatattta caacatgaac
      721 attacagacg gttcgggcac cggcaagggt cagaagttta atgcaacagt aacagtttcc
      781 gattttgatc tttcaggcgg cgaaatcgct aaaatcaagt ataaaatcgg atttaacggc
      841 gacccgacag aggttgatgt aaccgtcgca ggcggcgtta tcactgtcgg agcaacagca
      901 taatcgggag ggggctatat gctccctctt ctttttatag gaggatatta tggaacagtt
      961 aaggattcag cgtaaagatc tttatgaaat tcaggtcaat gacaacggag atacaattgt
     1021 ttttcagttg ggtgaccttg aactgccgtt taagcttgac aaagcgttta acgacgtcaa
     1081 caagatacag aatgacctga aaacccgtct tatcatcatt gataagcaga aggacagcaa
     1141 gggcaaaaat gaccttatga gcagaaatca gcgtgacaga atgaacgcat ggaagaatgc
     1201 atacagcaag atgcgcacgg cgatggacgg tttcctcggc gaaggcggct gtcagaaaat
     1261 tttcggcgac agcaattatc ttgaaatgtt cgatgatttg tttgatgaac ttgaccgtcc
     1321 gcaggctgac ggaaaatctc accttgagaa gatgaggctt tccgacgagg ccattgtcaa
     1381 gagaattgag gacaagtata agagcgcaaa gaacaagcag gtgatttaac gtgtacccta
     1441 cacacgcaga aatcaacggt gagctttatc cgataaacac cgattaccgt gtcgcaaagg
     1501 aatgctacga gatcatcaac gatgatacca tatctgatca agaaagagct ttagcggtta
     1561 tttataagct tttcggcttt gttccgcttg aaaacggtga ggcgttcctt gataaggatt
     1621 cgcttttctt gacttgcgga cagaagcagg aaaacaagtc agatgagcct cccgatttcg
     1681 atgtaaacga ggacgagggc tttattgaag cttcatttaa aagctgctac ggggttgatc
     1741 ttgatgaggt caatatgcac tggtggaagt ttaacgatct tgtacagggg cttgacaagc
     1801 attgcatact gtcgagagtg cgtgaaatca ggaattatga tttgaacgaa atcaaagacc
     1861 ctatacagcg gtcaaaaatg gctgaggcac aggaacggtt gaagcttccg gagagaatat
     1921 caacggagga aaaggaaaaa attgatgagt ttgacgctct gtttggaggt ggttaaatgg
     1981 cggcggacgg ttcaataaga gttgaaacga aacttgatac caagggcttt gaaaaaggct
     2041 tgaaagatgt cgataagatg tgtaacggca ctaaaaagca tcttgaaagc attgctgaaa
     2101 gcctgaatat caaaacaaat ccgattgaga ttataaataa ttctcagctt gcaaaagcaa
     2161 agaaaaaact tgaagaaatc aacgcagagg ttgaaaaaat acaagctcag acagacgaac
     2221 tgcttccaaa ggcagaaacg gaggaacagg ttgttaacct tctcaagctg gaggaggaac
     2281 aaacaaaaaa tcttgtggca gagcaaaaca atctcaacaa ggctatttcc gaatatgaag
     2341 ccaaacaaga aaaaatagct gccgaaaaac agcaaaagga gcttgagaga caaagacaag
     2401 cagagatcaa ggctgccgaa aaacagcaga aagacgatat taaatcaatc aattccgatg
     2461 tttccggcac ggtcgcaggt gatgattttg tt
//
LOCUS       DAWBSW010000243         2479 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6785_length_2479_cov_2.055693, whole genome shotgun sequence.
ACCESSION   DAWBSW010000243 DAWBSW010000000
VERSION     DAWBSW010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6785_length_2479_cov_2.055693"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    768..777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2032..2041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          664 a          467 c          547 g          781 t
ORIGIN      
        1 ttttccttga gaagcttccg cttatcttta aatgacttta aaatacaggc ccatggcaat
       61 catgggcttt tcgtcattat cggttctgtt tctctgcacc tattttgatg acaaattttc
      121 attcgtttca ataggtgcag gcattgtaat acttctgtta actcttgcga taggcaaact
      181 ccgtgagcgt gttacgcctt tctttattgc ggcggcgctc attctttcgg gagtttcctt
      241 cgaggtgctc agcgactata agatttccta tgccgacagc ctgacggaca aagaatccgt
      301 tattgaggcg acagttcttg acgagcctga atttaaaaat tcacggtatt attacacagt
      361 taaaaccgag aagattgacg gtgtgaattt tgaggtgaag ctcaggcttt ccgcttcgca
      421 gtttattgat acggaaccgt atgataagat tgagctggag gtcaatcttt atgaaatcgg
      481 ttcgttttca agagatataa agctttatta tcattcaaaa ggcatttttc tcggaggata
      541 tgtcagcaat tatgacggtt atccgattga aataataaag cccgaaaaca agccgcttgg
      601 ctatcattta ttacagttca gaaagaccgt tgagggcaga attctcgaca agctcccgaa
      661 tgactgcggc gcagtgaccg tcggaatgtt gacaggcaat aaggattata tttcaaacga
      721 gcttctcgga aatatcagag cggcgggagt cgcaccggtg ttcgctgnnn nnnnnnngca
      781 cttatccgtt tggataatgg gattgtttga gattcttgag cttttcggcg taagaaaaag
      841 actcaattcc gcagttggta ttgcttttac tcttttcttt atgggtttga ccggcttttc
      901 gccgtcggtt tgcagagcgg gaattatgct cttggttatt ctcgtaggca atttgttcag
      961 aagaaaagct gacgcaatta actcgctcgg ctttgcggcg ctgattctcg gaattataaa
     1021 tccgatgatt gttgccgata tcggattcct gctttcgttc tcatccacct tcggaatagt
     1081 tgcgctgaat ccgtggctga gaaagaagct tgaacctttg ctcgagggcg gtttcataaa
     1141 gaaattgata aaagcagcta ttgaatccgt ttatgtttca ttttgtgcaa ccgttgcatc
     1201 tttgggtttt ataatcgtgt ttatcggcta tgtttcggtc tattcgattt tttcaaatct
     1261 atcaataact tacgttgcat ctctttgtat gctctgcggc ggatttacgg ccttgcttta
     1321 tcctctcggc ggcgcaggca atcttttcgc cgtcgcgacg ggctgttttg caaaatatat
     1381 tataaaagtg gtcggtgatg ttacgcattg gccgtttgcg atgattgaca catcaaatgt
     1441 tttctggact tacggcgtga ttgcgtttta tgcgatcgtt gcaggctcat ttatcgtttt
     1501 caagaataag aaagcattca aggcttcctg tgtcggactt gcaatgacga tcgtattttg
     1561 cacattgctt tatcattttg aatattcggg ctatacctat ttcagagtac ttaacgttga
     1621 agacagtgtt gccgttatga tatcgcagga cggtaaaaac gcactgattt gctctcaaag
     1681 cgataataaa tacatttcat cgtctcttga tgatattaaa cccgatatta ttattgccga
     1741 tgtcgaaaac ggctgttcgt caggcgtttt atccttaata aaggaatata atcccgaaaa
     1801 ggtgattgtt tccgaagccg acaagtcttt tgaatcagtg ttaacacagg acaagatcac
     1861 aaaagcggag aatgcgaaaa ttaggctttt caacaatacg aacgctcaat ttgtttcaaa
     1921 ctcacattac agcgttgtat attgcaattc gcaggacgtt gagttttgta ttcttcttcg
     1981 cgttgacgat attgagaata ttcctgagaa atacctcagc ggcgatattt tnnnnnnnnn
     2041 ntatcggtac agagctgtat aaagattctg attttgaaaa agttcttata gccggagaaa
     2101 ctaagattaa gggcttgatt tcaacggaga aatacggaaa tatagatgta aaaataaaag
     2161 atgataatta taaaataatt gttgaggagg gttgataatg ccgcagctca gtgaaattga
     2221 attaaaaaaa caaataagct cgggtgattt tgcacgcttg tattttcttt acggaagcga
     2281 aaaatatctt ataagacatt ataccgccct catacggaaa aaagcgatcg atccctcatt
     2341 tgccgacttc aatttccatc agtatgacgg caaagagatc gattttgatg aggttgctca
     2401 ggcggcggaa gcgcttccga tgatgagcga tagaactctt atcttgataa aagatttcgc
     2461 cgcagacagt ctcaatgcc
//
LOCUS       DAWBSW010000244         2478 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6788_length_2478_cov_1.842344, whole genome shotgun sequence.
ACCESSION   DAWBSW010000244 DAWBSW010000000
VERSION     DAWBSW010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6788_length_2478_cov_1.842344"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    774..783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1949..1958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          672 a          480 c          575 g          721 t
ORIGIN      
        1 gcttatatcc gcatcggtaa atatgtttat cggaggaaag tttattgata agtcgtgggg
       61 agccaacgac ggaatagttc cgcttaagag tgctctttat ccgtttgagg aggatcacat
      121 cacatatgac gaagcaaaag tcattattcc cggagtatgg tatgttatgc ccacgattta
      181 tggcgcagat cattatgatt tttgcaatgc tgccgatgaa aaggccttcg gctcacgcca
      241 gggatttttc gatttttata tgaatctttc aaaactgatt tgcagtgttt gatnnnnnnn
      301 nnngctgtct ttcgtgacgg aggatggctt tttgacgacc gcaggcggag acttttcaaa
      361 ttatatgtaa agtccaaagg acggaaaaaa gcaaggtaat gacccgcgcc gttaaaagca
      421 ctttaaacac agttattgac ataaaattct gaaaaactgc aaaaaatcta tcggaaatct
      481 cagcactttt ataaaaaatt aacagaaaca gtcttgcaat aatcgaaaaa gtatgttata
      541 ataacttcgg gcattctata ctcaaatgcc ataatctatt aaggagaggt tgtttttatg
      601 tattgcaaaa actgtggagt aattctcaat ggtaaccagg aattctgtga aaactgtggc
      661 gttaaggtag gtaacggtga caagttctgc cagaattgcg gcggagagct tgctccggat
      721 gccaatactt gtgcaaaatg cggcgctgcc gtaaacggct ccgcaaataa ttcnnnnnnn
      781 nnngaatatc tcaacggcaa agataaaacc acgatggcaa ttatttgttt cttgctgggc
      841 ggcttcggtg ttcacaactt tatgatgggc gaaaccaaaa aaggtatcgt taaaattgta
      901 gcaagccttt gcttcggcct cggttacatt cttgcaatca ttgactttgt taaaatcctt
      961 accggcagct atgttgttga ccccgagaag ctgttctgag tgaagcttcg gtttaaggag
     1021 cttttgccga aattattctt cattcttttg cttggcatcg gttatttgat ttcgtctgtt
     1081 accggtatcg gatgcatatg gaagcatttt ttcggtatac agtgccccgg ctgcggcttt
     1141 acacggtcga ttttgtgtgc attacggttt gattttgaaa gagcatttca ttttcacccg
     1201 atgttttggt cgtttccgtt tatggggata tatttcttaa tcggagacag agtaaaaagc
     1261 agattgctca acggtattat gcttactgtt gtgatcggct tcttagcgac atgggcaata
     1321 agagatattt ttaagttgct tgcatgagct gtttaacaat tcaggttaaa atcccgttct
     1381 gtcgagcggg attttttgtt ttacagcttg ataaatccgc cattgttatt atttgtaatc
     1441 ttattcagcc gtattcattt gcggtgtaag ggaagaaaat atagattatg atgcattcaa
     1501 agtagcagta atcccggcat cgcattatgc aagtaaaacg gcgcttaggg gtttcggcgt
     1561 aattatgctt gagtcgcctt caaaggaaaa gctcggagcg tgaaaaagat atccgagcgg
     1621 agcaaagagc atggcatttc gagcagcggt caacgtgtct cgcgtaataa ttataataaa
     1681 aggcttcctc aaaaatcgaa tgaggaagcc tttggtgttg tattaaactg aagacagaga
     1741 cgaaacggtg tctccgaccg ctttcaaaag cgagtgcgga ttgctcgacg gaagatcaca
     1801 gctgtaatgc caaatcatta ctccgccaaa gcctttttcg actgcaaacc gcgttttctc
     1861 ggcgatttcc gacgggcggt tgaaccaaaa tgctttgtca agctcgttgt cataataaaa
     1921 accgttttcg ttaagcttgt cgcaatagnn nnnnnnnncg gttctgtcgg acggcttcgc
     1981 gtaaaacggg agacctaggt cgatcttttc ggacggaatc gaatatcttc tcagcttttt
     2041 tgaaagctcc atgaaggttt cgggtgtcga atgtcttcat tcgtcgtcat aaatgtcata
     2101 aagcataatt tcaaagcgat cgacggcagt atatgaaccg ggagaaagct tgatgtccca
     2161 ctcggaaacg gcgagcccga gaattttttt gccgagcttt gcgtcaaggc ggataagaaa
     2221 ccggttgaac ggagtccagt ttgtataatc catcgggtat tcatagtcaa aatacacgcc
     2281 gtcaaaatca tatttgtcaa cgagcgcgat tatattatcc tcaagtacgc cgcttttgaa
     2341 agcctttgta tgctgctctc cctggtctct catagccttg ctgtaatcat cgtggctttc
     2401 gagcggaccg gggccgagaa gattcacatg gatcgaaacg tcgcgctcgc cgatcacggc
     2461 acgaaggttt gaaagagc
//
LOCUS       DAWBSW010000245         2466 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6822_length_2466_cov_2.289092, whole genome shotgun sequence.
ACCESSION   DAWBSW010000245 DAWBSW010000000
VERSION     DAWBSW010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6822_length_2466_cov_2.289092"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1714..1723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          696 a          521 c          633 g          606 t
ORIGIN      
        1 actttcaata attcctacgg gggtatataa aaatggttaa ggatcttttt aaaaactgga
       61 acacaattcc gaactggctt tcatttgcaa gaatcattat ggttcctgtc tttgcatatc
      121 tttttctcaa tgattctatc ccgaacaata ttatctgggc ggtgttcgtg cttgctcttt
      181 ccggactttc cgattgcctt gacggcaaga tagcacgccg ctttaatcaa ataagcgatc
      241 tcggcaaggt gcttgatccc gtggcggata agctgactca gataacaatt gccgttcttc
      301 tttacatcag gttcagctcc agcacggatt cgcttatcaa aacattcagt tggatattcc
      361 ttgtttttat tataaaagag tttgtaatgg tcctcttcgg aggaattatg atagccatag
      421 gtctcaaacc gagtgcggct gagatttacg gcaaggttgc gacctgcgta ttttacttcg
      481 taatgatagc gctgtttgct ttcgctcccg atgtaggagc cttcaacagc ctttggacaa
      541 ttcccgatac aatgctcatc gtgctcgttg cgatttcggc attgctgacg gttgtggcat
      601 ttgtcagcta tcttcccgga gtgtttaagc agttcaaaga aaaaatcagc aaaaaataac
      661 aggagataaa tatgaagcaa ataatgtgca gcaaatgcgg catcagacct gctgttatat
      721 tcatgacaag aatggacggc gacaaaagct cccatcagga ggggctttgc ctgaaatgtg
      781 ccatggagtc tacgggtccg ataaagcaaa tgatggaaaa catgggaata acggaggacg
      841 actacgaagc aatgagcgag cagttcggcg ccatgatggg catgggcgac gagggcgagg
      901 gcttcgagcc gggcggaacg ccgaccttcc cgttcatgca gggctttatg aataaggacg
      961 agcaggacga tttcaccgat gttcccgata cggaagttaa agaaaaaagc aaaggcaaga
     1021 ataaaggcga aaagaagtcg aaaaagcgca aatatcttcc gctttattgc cccaacctta
     1081 aaagaaaagc aaaagagggc aaaattgaca gaataatcgg cagagacaac gaaatttaca
     1141 gaacgattca aattctttgc cgccgatcaa agaacaatcc ctgtcttata ggtgagccgg
     1201 gagtcggtaa aaccgctatt gcagagggct tggctctcag aattgccaac ggtgaggttc
     1261 cggcaaagct tgccaataag gaaattcagc ttctcgacct cacctcgctt gttgccggta
     1321 cgcagttcag aggtcagttt gagggcaggg taaagggtct tgtcgaagag gttaaggctg
     1381 agggcaacat tattctcttt attgatgaag tgcataacct tgtcggtact ggcggagact
     1441 ccgaaggctc aatgaatgcc gccaatattc tcaaacctgc tctttcaagg ggcgaggttc
     1501 agattatcgg cgcaacgacc tttacagaat acagaaagta tattgagaag gatgccgccc
     1561 tcgaaagacg cttgcagccc gttaaggttg aggagccgtc gattgaggac acctataaaa
     1621 tgcttctcgg cataaaggat tattacgaga attatcataa ggttcagata agcgatactc
     1681 ttgttcacag cgcagttacg ctctctgaga gatnnnnnnn nnngctattg acctgcttga
     1741 tgaagcctgc acctgtgcaa acctgagaaa caagaacatc agcgaatatg aaaataagct
     1801 caatgaaaag gcggatctta taattcgtga gcaggatctc atggaggctg aggagccgga
     1861 ttacgaggcg attgccaagg ttaagcagga tataatgcag tgcgagacaa gaataaatca
     1921 gcttatcccg ctggttgaaa attctccggt tacagaggag gatctcgcaa gggtaattca
     1981 gctttggaca ggcattccgg cgcagaagat agttgaaagc gaccttcgcc gacttgccga
     2041 tctcgacaag aagctcaagg caaaggtcat tggccaggac gaggcggttg acgttgtttc
     2101 gaaagccgtc agacgcggaa gaatatgcgt cagcccgaga agaaggccgg catcgtttat
     2161 ctttgtcgga ccgacgggtg tcggaaaaac cgagcttgta aagcagcttg caaacgaact
     2221 ttttgacacg cctgagactc ttatcaggct tgatatgtcc gagtttatgg agaagcacag
     2281 cgtttcgaga attatcggtt cgccgccggg ctatgtcggc tatgatgagg cgggtcagtt
     2341 gacagagaag gttcgccgta agccgtattc ggttctcctt tttgatgaga tagaaaaggc
     2401 gcatcccgac gttatgaaca ttcttcttca gattcttgat gagggcaaga tcaccgatgc
     2461 gcaggg
//
LOCUS       DAWBSW010000246         2462 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6841_length_2462_cov_2.528459, whole genome shotgun sequence.
ACCESSION   DAWBSW010000246 DAWBSW010000000
VERSION     DAWBSW010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6841_length_2462_cov_2.528459"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          693 a          538 c          657 g          574 t
ORIGIN      
        1 agccggagcg tgaccgagcc gcagcgagac gctggcggtg aagccgactg aggggttttg
       61 caaaaaatct gatgagattt tctttgagcg tgggtacctc ccacgggtgc gctgtaaagt
      121 tgaactgttt tcagtctccg cttgaatgac ccaaggtaag aaaaaaccgc caatacttct
      181 ttgctgtgag aagtattatg gagatgatat tatgaacata acaataaaaa aaggaacggc
      241 gtccggagac gttttgattc cgtcgtcaaa gagtgttgct catcgcttgc ttatcaatgc
      301 cgctctgacc gacggcgaaa ctataatcaa cggtctgtcg ctcaatgagg acatcggcgc
      361 aacggttgac tgcctcagaa cgctcggcgc aggaattgaa tattccgaag gcaggtgcac
      421 ggttcacgga atgaacggca ggggagagac cccgcgcagg ctttattgca gagagagcgg
      481 aagcacgctt cggtttatga ttccgctttg tctcgactcg gtgaaaacgg agctttacgg
      541 ttcgccgagg cttatcgaaa gacctcattc cgtttacagt gaaatctgta aaaaaaacgg
      601 ctttttatgg cagtcggacg gtgaaaaaat caccgtttgc ggcggcttga aggcagggag
      661 atacgaggtt ccgggcaaca taagcagtca gtttataacg ggtcttatgc ttgcacttcc
      721 gacccttgac ggtgacagcg tgataaggct cacaaccgag ccggagagcc tttcgtatat
      781 cgacatgact gttgcttcgc ttgccgcttt cggaatcgtt attgaaaaga gatccgagcg
      841 tgagtggttt ataaaaggct cgcaaattcg tcggagtccg ggcgtaataa acgttgaagc
      901 ggacgaatcg ggtgcggctt tcttcggcgc attgaatttc atcggcggca atgtgaagct
      961 cgacaatctc aatgaaaatt catgtcaggg agacagggtg tggaattcgc ttttcccgat
     1021 gcttaaatcg ggcaagcctg agatttcaat caaggactgc cccgacctcg gaccgatact
     1081 catggcactt gccgcctgtc ttaacggtgt gactctcacc gacacggcaa ggctgaaaat
     1141 caaggagagc gacaggggaa atgcaatggc ttgtgagctt gaaaagctcg gcgtcaaggt
     1201 cacggtctcg gaaaacacga taaccgttga cccgtccggt atccatgcgc cgaatgaagc
     1261 tcttgacggt cacaacgacc acagaatagt aatgtcgctt gcagttctgc tgacgaggtt
     1321 cggcggcaga atcaacggag ctcaggcggt ttcaaagagt atgccgcagt ttttcacgat
     1381 gctcaattct ctcggaatag aaacagaaga aggttaatat gaaacagata aataacaaaa
     1441 caaaaattct catagtcggt ctcggactta tcggcggcag ctatgctaag gcgctgacta
     1501 aaaagggcta caaggtcagg gcaattaccc tcgacagcag tgacattgaa tatgcaatag
     1561 acaagggctt cattgaggac ggtacaacgg cggttacaaa ggagtatctt gcatgggcgg
     1621 acgtgatagt tttttcactt tatccgcaca cgtttatcga atggctgaaa aaatacggtt
     1681 caatgattaa aaaaggaacg ctttgcaccg acgtaacggg tgtcaaaagc tgtattcttt
     1741 cggatgcaca ggcgcttatg cccgagggcg tcgagttcat tgcggctcac ccgatggcag
     1801 gccgtgagcg cagcggagta agacacagcg acgaatcggt ttttcacggc gcaaactaca
     1861 ttgttgtacc caccgagaaa aattccgagg aagcgattga atgttgcaaa aagctcggcg
     1921 aggagcttgg cttttaccgc atcagtgtgc ttgatgcaga atcccatgac cgaatgattg
     1981 cttttctttc gcagttgacc cactgtattg ccgtctcgct gatgtgcacg aacgacactc
     2041 cgggatttga ggcttacacg ggagactcgt tccgtgacct cacgagaatc gccaaaatca
     2101 acggtgaaat gtggagtgaa ctttttatca gaaacaagcg ttttcttctt gatgagatgg
     2161 acaatttcat caatcagttt acaacgctca gaaatatgct tgaaaatgag gataaagagg
     2221 gtatgtgcga aatgatgagg atttccacac agcgaagaga gaaattcgat aaaccgaaag
     2281 caaattaaag caaaggattg atatattatt atgaacatga aattttacca taagctgccg
     2341 ataccgcagg acgtaaaagc gcactatccc gtgaccgaca gcatgaaggc tgtcaaggca
     2401 aagcgagaca aagaaatcaa ggacgttttt gagggcaaga gcgacaagtt tctgcttgtg
     2461 at
//
LOCUS       DAWBSW010000247         2440 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_6927_length_2440_cov_2.778616, whole genome shotgun sequence.
ACCESSION   DAWBSW010000247 DAWBSW010000000
VERSION     DAWBSW010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6927_length_2440_cov_2.778616"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    664..673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          684 a          536 c          622 g          588 t
ORIGIN      
        1 atacaaggga ggcaaataaa tgaaaaaaca aacaatgaaa cggattttta ccgtcatttt
       61 ggcggtgtgt atgctcctga gcattgcacc tgttacgatg gctgccgaca cggcggacgg
      121 cggttatcag aagggggata taatcgaatt cggttcatat cctcagtcaa aggtgaccaa
      181 tccaaggatc atttcggcac ttgacggctt cctgaccgac gacgtgtggg tgtcatatga
      241 ctactacatc ggcacggaca cggagtatga cggcaaaatg acagcgtcgg actacatgaa
      301 gtatgccgat cttgagctga acggcgaaaa ataccgcgcg gtcagatttt cgcagtaccg
      361 tccgaaattg acgagcagta cttcgagtga aacaaattca atgcaggata acaacggcta
      421 ctatttaaac accacatact tctttaaata tgagccgctg acatggacgg ttcttgaccc
      481 cgaagagggc tttgttatgt ccaatattgc cattgacgct caggcttttg agaacttttc
      541 ctattatgat cccgactctt atgattccgc attgggacaa agggtttata atagcaagga
      601 ctgcaagtat ttagccaacg attggtcaaa aagctctctg agagagtggc tgaataagga
      661 tttnnnnnnn nnnctacaac accgctttca cggcggagga aaaggctcag ataggtaaaa
      721 cctatcttga caacaacaat ccgtttagtc cgaaatatga ctgcgccggc acatttgata
      781 agatttttac aattatcttt tatgatgcaa taaacagcga ttatggcttt gatgagaacg
      841 gatcacgtag cgatcttgca agaataaaac cggctacgga ttatgcaatg tgccaaggtg
      901 cagatgttgt ttcgtataca ggcggcaaca ttccgtattg gtggctgcgt acaacaggtc
      961 actacggcgc attcgccttt ggcgtagctc cgggcggctg gatcgtttcc agcggcagcg
     1021 gttattacaa tgtcaacaga accgatatgg gcgttgttcc tgcgttcaag ttcaatccga
     1081 agacgccgac gctcgctaag ggcagcaaaa tcgaattcgg ctcatatcct cagtccgagg
     1141 tcacggacgc cgatgaggtc gcaaagcttg aagcggactc ggagaattat ctttgggttt
     1201 cctataaata ttacagcgga acaggcaata gcacggacgg caaaatggag ccgggcgatt
     1261 tcatgctgta taaggatttc tacagcggcg gcgaaatgtt ccgcgcggtt actttttcgg
     1321 agtacagacc gggcaccggc ggcggattaa aaggagatgt tttttctaac cagaaagaca
     1381 acgactatca cccgaacaat gtatattatt ttaaatacga accgctgaca tggcgtgtgc
     1441 ttgaccccga cgaggggtat attatgtgcg ataacgtaat tgattcgcag tcttatcaga
     1501 atttcgttat aagaaaagac gataagcttt ataacagcaa ggattgcaca aattatgtct
     1561 cagattggga aacctgctca ttgagacagt ggctcaacaa gaccttttat aacacggcat
     1621 tctcccgtga ggagaagatg ctgataggca caacattcct tgagaataac agcccggacg
     1681 gcacttggtt cggcaccgat acgggcgata aaatatttat tctctcgttt gacgatgtaa
     1741 ttaacagcgc atacggcttc gactcatcaa aatcggaatt tgacgaagca agaaagctca
     1801 aaggtacgga ttattcaaaa tgtcagggcg tatatatcag ccagggttat ccgcattggt
     1861 ggcttcgtac cccgggcaat gccgaagcgg tttacctagt cagcagctac ggctgggcgg
     1921 ctctcggtca gggagtttac ggcacaggcg tgggcgttgt tcctgcactc aaattaaacc
     1981 cgaaggccgt tgatgtcggc gttgtttacg accttgacgg cggtgcatgg gcagagggct
     2041 acactgcacc gacaagctat aaatcaaatg aaacacttgc actcccgacg gcggaaaacg
     2101 ttgtaagagc gggctatacc ttctccggct gggagcagac gagcttcata aacaagactg
     2161 ttacctatgc ggcaaagtgg atatccaacg gttataccgt aagttttgat tcaaacggcg
     2221 gcacggcggt tgcggataag tctcttaaat ggagcgacaa ggtgctcgaa ggcgtttcgg
     2281 atccgacaaa ggcaggctac acattcacag gctggaagta cgctgattcg gcagtttcgt
     2341 cagatacaaa gttttccgac ctcgctgcag atgataaggt aagcggaatt accctggtgg
     2401 cagagtggac ggcaaaaacc tatacgatta agtttgatac
//
LOCUS       DAWBSW010000248         2419 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7013_length_2419_cov_2.349831, whole genome shotgun sequence.
ACCESSION   DAWBSW010000248 DAWBSW010000000
VERSION     DAWBSW010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7013_length_2419_cov_2.349831"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    341..350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1849..1858
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          710 a          473 c          572 g          644 t
ORIGIN      
        1 atttgagctt gacggacata agtgcggaat tgttatctgc tacgatatcc gattctgcga
       61 gcttatcaga agccttgcgc tcaagggcat cgacatgctc tttatggtgt cccaatggcc
      121 actggtcaga acctctcata ttgttacgct cacaagggcg agagcgattg aaaatcagat
      181 gtttgttgtc tgctgcaatt cctgcggcaa aactccggac actgtattcg gaggccattc
      241 gcagataatc gacccgtggg gcgaggtgct cggtgcggca ggcgagcaac aggaggttat
      301 aacagccgac tgcgatttcg gaataattaa ggatatcaga nnnnnnnnnn acccgatctc
      361 tatgaattat aataatgctt tgaaaggagc gacagaaatg tataaatttc cagtaacaag
      421 aactacgcac ccaaaggaga agccggatca gaagaagctc ggcttcggta agtatttcac
      481 agaccatatg tttatgatcg actatgatga gggtcagggc tggcacgacg gaagaattgt
      541 tccgcatgag ccgatcgcca ttgagcctgc atcatgcgtg ctccactatg cccagatgat
      601 gttcgagggc atgaaggcat ataaaactcc gtcgggcgac attcagcttt tcagaccgga
      661 tcagaacttt gagcgaatga acagaaccaa tgaccgtatg tgtatccctc atatggaaaa
      721 ggacatcttc tttgacgcta tcaatcagct tatctccgtt gataaggact gggttccttc
      781 tgagccgggc acagctcttt atatcagacc gttcatcttc ggcgatgagg tttcgttcag
      841 cgttcttccg gcaaagcatt atcgttttat catcattctt tgtccggttg gttcttacta
      901 tgccgcaaac gacggcggac ttcatacaac gagaatttat gttgaggacg aatttatccg
      961 tgcggctgtc ggcggcacag gcttcgcaaa gtgcggcggc aactatgccg gcggcatgaa
     1021 ggcttcggca aaggctatgg agcatgactg caaggacgtt ctttggctcg atgccgcaga
     1081 gcataaatat gttgaggaaa tcggcacttc aaacgcattc tttttaatcg gcgacgaggt
     1141 tatcaccgct ccgcttatgg gtacgattct tcccggtatc acaagaaaat ccgttattga
     1201 gcttctcaaa aagtggggag taaaggtttc ggagagacgt ctttcaatcg acgatgtatg
     1261 cgccgcttca aaagcaggta ctcttaaaga ggtattcgca agcggtacgg ctgcagttat
     1321 ctcaccgata ggaactcttt actacatggg atcggattac aaggttgccg acggcaatgt
     1381 aggtcctatc gctcagaagc tttacgacac gctttacggt atgcagacag gtcacgttaa
     1441 ggacgacatg ggctggacat acaaggtaca gcttgactga tttttgatat aataacggcg
     1501 gaaatcgttt atcggtttcc gtcatttttt attgccgttt tccatatatt atatagggag
     1561 ttatatgaga caaaggttag atgaaaatat gttcggttcc attgtatgga aagtcagaat
     1621 gtttttacac ctaaatcaaa ttttataaga tttttcactg taatgaggtt cagaaaacat
     1681 acaattcgta ggggtcgatg cccacatcga cccaaggtta gaataaatca ttattgagcg
     1741 tggttctgtc tacagaggag ctttcacggc ttgtccgtga ctgagggagt gtttcaatgg
     1801 gaaagctgaa gaaaaaattc agcatgttca attacacgaa gaagtggann nnnnnnnngt
     1861 ctaatatgga tttccgcaag ctatattctt gccgttttcg gaagaaatga gatcgcagaa
     1921 acgctcggcg gggcggtcat tgaccttacg aaagcaattt tttgcaccta tattgtcaga
     1981 gctttttttg atacatactg cgaaaaaaac tattgcctga aaatgtatcg tgaatgtcag
     2041 ggaaagaaat ccgataatat cgaaaacagc acagaaaaag acaattgtga cggtgaaaag
     2101 agtaatttat aaaccttaaa cagaagtata aaaaaacatg gtcttgtcaa aatcacaata
     2161 tggaattggg gtaaaatatc acaaaatgca tcttattgtg tctgaataac atatatattg
     2221 tgatgaaatt actttaaaaa tttgacaaag tctgcgagtt atgataccat gaacgcaaca
     2281 gaaatgacgg atgtgaaaca atggcagatg agaataaaaa ttcttgcgaa aactgtaaat
     2341 atttttccga cggattgagt gaaaaaggct attgtaagct ttatcatcac aacctaaccg
     2401 agccggaaag aatttgcag
//
LOCUS       DAWBSW010000249         2378 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7162_length_2378_cov_2.504950, whole genome shotgun sequence.
ACCESSION   DAWBSW010000249 DAWBSW010000000
VERSION     DAWBSW010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7162_length_2378_cov_2.504950"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1978..1999
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          638 a          578 c          493 g          647 t
ORIGIN      
        1 ggatccataa gcggttcggt gagctttgag gctatcgccg aatatgccgc ctccgggaaa
       61 acgcagtcaa ctattccaac accgctttca accgccgcat ttgccgccat aactgccgct
      121 ccctgcatat tacgactgcc gcaaacgctc agaagtctgc cgaaatcgcc cttatttgcc
      181 gcgggatttc tcggtttaaa aaagcgtttt atctcattat gctccgcaga aacgatcacg
      241 tccgagccta ccgcatcgta gcatttttcg tcaatgccga tgtcaataag cgacacgatt
      301 ccgcactggt ccgaagccgg tttaagaata tgcacgggct tgtagcaggt tatcgcaaga
      361 gtatagtctg ccttgaaaaa gtctgattta atctcgccgc tgttaccctc aagtccgctc
      421 gggatatcga cggcaaactt tttggctctt gaccttgata tatcattaaa aaatttcgat
      481 gttgtttcgt caagccggcc gtgaaatcct attccgaaaa cacagtcaac aaccgccgcc
      541 gcactcatac atgcagaaac gcaggcatcg taattctgct tgaggtcgga gacaagcacc
      601 ggcttatcct caagataagc atacatgatt ttggactcat atgccgtcgg atagccgttc
      661 gtcagcacaa gccggatacc gtagcccttg tcgctgagat tttttgcaat aacaaaaccg
      721 tcgccgccgt tttttcccga gccgcagaga accgcaaccg tcttggccgc agaattcaag
      781 ccgacaatgg ttgagtggat tttttcggcg caggcattgc cggcattttc catcatttca
      841 acatacgaaa gtccgttaac atccgcattc tcctcgatga cacgcatctg ttcacaggtg
      901 taaatgagca tattgagtct ccttaaatag tgtttgttct gtaaagtaat tctgtgcatt
      961 tatcagcctt tttctcaagg taaagcctgt ttattgcatg tatcttcaaa gaattacaaa
     1021 ttctccacat accaatcaat cgccagcttc aaaccggaat caaaatcata atcgggatca
     1081 tatccgagca tttcacgtgc ctttgatata tcggcgttgg agtgttttat atcccctgcc
     1141 ctgtcgggtc cgaaattcgg ctcgatatcc ttgtcgagag cgtgggtcag cgaatgataa
     1201 atatcaatca ggtattcacg tccgccgtag gctatgttga aggcattgcc tgccgcctcg
     1261 tgaggtgcaa gacaagcctt gagattcgcc tcgataacat tctcaatata tgtaaaatct
     1321 ctcgattgca ttccgtcgcc gttgattgtc ggtgtttcgc cgttcatgag cattttgata
     1381 aacttcggaa taaccgccgc ataagcgccg tccggatcct gacgcctgcc gaaaacgttg
     1441 aaatatctca agccgtaggt atcgagtccg tagagccttg tataaagctt agcgaattct
     1501 tcatcaactc gcttcgtcag agcataggga gaaagcagat tgccctcctg accctctttt
     1561 ttcggaagta cagggtggtc gccgtaaaca gaagagcttg aagcataaac aaatttttta
     1621 acgccctgct gtcttgccgc ttccatcatg ttgagagtac ccatgatatt gtttttctca
     1681 tagaaaagcg gcatttcaat gcttctcggc acactgcccc atgccgcctg attgagaacg
     1741 taatcgacgc cttcgcacgc tgccatgcag gtgtcgagat ccttgatatc accctttata
     1801 aacgtataat tcgggttatc aagaaaaatg tcaacgttcg cctgcttgcc tgttgaaagg
     1861 tcgtcaagac atctgacctt atatcccatt ttcaatattg cttcgcaaag gtttgagccg
     1921 atgaagcccg ctcctcccgt tacaagaaag gtcgaatttt ccgaaaattt caaatttnnn
     1981 nnnnnnnnnn nnnnnnnnnc catagtgtca ttcctcattc ggcggcaaat aaacgcaccc
     2041 gacgattata tttatttaat tatatcattt tcacctcttt agtgcaagaa aaaatgcact
     2101 ttttgcggtt aacaaggttc ttttcgttgc aaaaaagcat tttttgtgct attatagatt
     2161 cagtaaccac ttattaagag cggaggcacg caaatgagca acaatattcc aattaaaaca
     2221 agagcagtcg gcggatttga taaaaaacag gtcgaccaat atctaagctc actcgatgag
     2281 gaatatgaaa agtcggtttc aaaagaggat attgcaaagc tacgcgaaga gatagccgag
     2341 ctgaaaaaat ctatacgtga aaaagataaa aagctctc
//
LOCUS       DAWBSW010000250         2378 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7161_length_2378_cov_2.751184, whole genome shotgun sequence.
ACCESSION   DAWBSW010000250 DAWBSW010000000
VERSION     DAWBSW010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7161_length_2378_cov_2.751184"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          607 a          489 c          608 g          674 t
ORIGIN      
        1 cagatgaccg aggataagct gagcaacatt tggcgtgacc tgcttgaggg tgatattgat
       61 gtacttgttt gtacgacgat tattgaaacc ggtgttgatg tgcctaatgt caacacgttg
      121 ataatcgagg atgcagacag gctcggtctt gcccagcttc atcagataag gggacgtggc
      181 ggccgttcgt caagacgtgc aagtgcttat ttcacgttca gacgcggcaa ggagctgacg
      241 gaggttgccg caaagcgact gacggcaatt cgtgaatata ccgagttcgg ttcgggcttc
      301 catatcgcga tgcgtgacct tgaaatcaga ggtgccggaa acattctcgg tgcacagcag
      361 cacggccata tggaatcggt cggctatgat atgtatttaa aaatgcttga tcaagccgtt
      421 cgtgaggaga aaggcgagat ttctgctgtt cccgaaaaag aatgtcttat tgatctgccg
      481 atcgacgcac atattcctgc cgattacatt gaggatgttg cgcagaagct tgcgatgtat
      541 cgcaggattg cggacatcaa aaaccatgat gatgccgatg atgttctcga cgagcttata
      601 gaccgcttcg gtgaaccgcc gcagtgcgtt cagggcctta tcactgtttc gcttctcaga
      661 aatactgcgt tacagcaggg cgtttatgag atagggcgaa cgggcagtaa cgtcaagctg
      721 tttatagaaa cgcttgatat ggaaaagata tcgaatcttg caaaatatat gcgaggcagg
      781 ataacggttt ccgccgcagg caagccacac atagccgtta aggttatgcc gggcgaggat
      841 cagttggcgg ttataaataa ggcacttgag ataatgtcaa aatagggttg acgccttttg
      901 caaataaaac gttttctgta tcagaattcg acgaaccata cggatatgaa aaaagcttcc
      961 ttgaaaaaag gaagcttttt atgtgtttat tcaaatgaca agccgtacgg ccattcgacg
     1021 agactgccga catcgtaaac gtttgtgtag ccgagctcgc acagtgcttc ggcggcaagc
     1081 ttggcacgct cgcccgtttt gcaatagaca ataatcggag tgtccttatt cggaataagc
     1141 tcggcggcgg tttcggcatt gatcgtgtca acgtcaaagt tgaccgctcc ttcggcatgc
     1201 cccgtgaaga attcttcatc ggtacgcacg tcaaacatta tacagccgcc ttcgtcgagc
     1261 atagtttttg cttcttcaaa tgttattctt gtgtatgtaa tcatgttgac ctcactcggt
     1321 aagattgttt ggcatttttg gctttgcttg cgcttgtggc ggaagggata aaatacaacc
     1381 ttaataataa tctattttcc tttgttagtc aacccgtgaa gcttgcaccg agatcctcgc
     1441 cgaaaccgtt gcaggtataa cggaatttta tatcatcgcc gtttttgacg gtgtatttat
     1501 taacgccgta attcgggaaa actccgttga ccgaatacat ccagccggat ttagtgccgc
     1561 agtccttttc gtagagctga tgaattcccc tgatatattc gctgtcataa ccgggagtgt
     1621 aaacatattc aagctgaatt cccgatttac ggcagtaggt gcatttctcg gggcaggtgt
     1681 tctgcttgca aacgagcctc agtacgtcaa aaactgtgct gccctcggcg acggaaacgg
     1741 ttgtttcttt aagaatatat ccgtccttcg gcagaaacgc tttcttttcg ggacgaagat
     1801 taccgagatt gttgagtatt gttttacatt cgatttcgat tgtgcacgtt acggtttttt
     1861 cctgcttctt tgtcgtggtg accttggagg gcttggacgt gtgtgccgtt gttgtaattt
     1921 tggcggttgt tgtgttcgat gcagttgtgg tataacccgt cgttgaggtt ataacggaat
     1981 ccgttgaaga tattgtgctt tcgtcggtaa ttatgttttg ctcatcggct gttgtgttct
     2041 cgatcttggt cacggtaatt ccgcagcccg acaagagaat taaaacagaa ataagcagaa
     2101 gagaagtata ttttttcata tcggtaaatc aatcctttaa agtaatcgac ggttggtcat
     2161 gtgccgaaaa gtccgtattt ttttgctatt cggtcgaatg tatcctgaat ataaggcgtt
     2221 gcaacaaaaa gcgcaatgag cgttgttacg gcatgaattg cgttgaagct catacccgag
     2281 atataaacag ggagcatggt tttaactgtc ggtgcggcgg agaaaaaaag agcgcagctt
     2341 gaatcgagaa taaagccgta aacggcgaag cacaatat
//
LOCUS       DAWBSW010000251         2346 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7285_length_2346_cov_2.443474, whole genome shotgun sequence.
ACCESSION   DAWBSW010000251 DAWBSW010000000
VERSION     DAWBSW010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_2346_cov_2.443474"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          602 a          544 c          448 g          752 t
ORIGIN      
        1 cctacgtctg tagttcgatt gtatcatatt gttattttta tgtcaatacg cttaaaaaat
       61 attctttatt gtcggttttt cgctctcttt tatatgttca aggcgattta ttacgctgtt
      121 tatttcctcc aaatattcgt cggtcagccc ctgttcatgg tagttttcaa tattttttat
      181 tccgatttca atatcgtcaa gctctgagtg cggcaagaag gacaccataa aagtctccga
      241 tctgtcccat gcgtcggaaa gacgttttgt ttcccaaaaa tctgttttct ccttttgacc
      301 gacggactcc cgaatctcgc aggcaatatc tgtcagcctg tcgacctcgg aaataatctt
      361 aaaatatata ccgatcgacg agcccaacac aataagtaaa atcacagtgg cggcaattat
      421 tcttctcatt gatattcttc tttcttcact atcgtatagt tgccctcttt atccgcaacc
      481 atgaaaagca cctcgttaat ttgcgtgttc tccgagtcaa gaatattgta tagcttttcc
      541 tcgttcatgt cacattcttt aaatgagcgt ttgtgaaatt taccgtcgtt tatcaccatg
      601 caaagcagtc cctttgtttc gttttgaata tcagccattt ccgccgtgac ggtctccttt
      661 tcgggcttta gcataacgct gagctgtccg ttcgtttcga caatcgcata gcgtacatcg
      721 gagatatcga acacatcctt ttgcctgagc tggtcaaaaa gatcgtccat agtaaatcgc
      781 agccttttca actgcttttg gtcaatcact ccgtcacgga taacgacgat ggaattgcct
      841 tgcaaaaagc tgcggcacct tgagcttttg agcacaacaa cggaattgat tacctccagc
      901 gcaacgagaa gcagaaccgc aacgattgaa ttgccgagcg gaagtgcgtt atcctgcatc
      961 ggaatcgccg caatttcaga aagaagtatg gttattacaa gctctgtcgg ctgcaattcg
     1021 ccgagctgcc ttttgcccat gatgcgcata catatgatta tcagtacata taaaatcagc
     1081 gcacgaacca atgttacaag catatttatc accgataata tgattgacga aaatccgcaa
     1141 aataaacaaa acaccgaaca tcatgtgttc agtgtttcaa ctatttttct catatcctca
     1201 ggcatgggtg cttcgagggt cattctctcc cctgtcacgg ggtggacaaa gctcaccttg
     1261 gcgcaatgca gtagctgatg tccgagcttc atttcgtccg tgccgtacat accgtcgccg
     1321 acaagcggca tacctattga agcaaaatgt actcttatct gatgcgttct gcctgtttcg
     1381 agccatactc ttacaagaga tttttcgccg tccgttgcga tagtctccca atgggtaacg
     1441 gctctgtcac cttgctcacc gaccgctctc agtgttttca tcgggtcggg tcggtaaatc
     1501 ggcttatcta tcgtgccgaa tttttccgtt ttgcccgaaa caactgcaag atattccttt
     1561 tccgccgttt tcggaatttt ggcagcgctg tgcttgttca aggcgcagat aactattccc
     1621 gacgtaccct tatcaagcct accgaccgct ctgaaaaccg actgcttgcc ttcatttttt
     1681 aaatgataag cgagagcatt tgcaagagtg tctccctggt gattatgcgt cgggtggcag
     1741 gcaagaaacg gtgatttatt gactgcaatc aggtcgtcat cctcgtatat aacgtcaatc
     1801 ggaatattca tcggctcgat ttcaccgtcg ggacacggga tctcgatctt caattcatcg
     1861 cccgtgtgca aaatatctac cgttctgata tgctcgccgt tgagcgttat tccattttct
     1921 gttcttttca gacttcttaa aagccgagcc gagattttcg cctctccttt aagatacgca
     1981 atgacttttt ttccgtcaaa ctcctcggga atcttatatt ttatggttct tttcattcta
     2041 tcaccgagga tattatacta tatttaataa attattcaag taaaaatgta aaaggcaata
     2101 ttgaatatgg tcagaattat cctgtttttt tgcataattc gttatatttt gtacataata
     2161 acaaaagttt aaaaaatggg ttaaaaatat aacaaatcac ttgcatattt cgctttttcg
     2221 ttataaaata tcaatggtaa aatttaatac taaggaaatt acggcttggg atattctttc
     2281 gggtcttcgg actcggacga gcctcccctg tcgtcgtttt ctaaaaattt ggaggtaatt
     2341 caaatg
//
LOCUS       DAWBSW010000252         2324 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7374_length_2324_cov_2.081534, whole genome shotgun sequence.
ACCESSION   DAWBSW010000252 DAWBSW010000000
VERSION     DAWBSW010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_2324_cov_2.081534"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          594 c          431 g          723 t
ORIGIN      
        1 cagtcatttt gtagctccgt aattatttaa taattaacaa tattaaagat ccttgatctc
       61 gtcgggctca acgatttcgt attttttgtt gagaatttcg tcaagagcaa tgcttacagg
      121 cttctcaaca aggataattc cttcatcttc agcttcctcg gaaatctctc ttgcacgttt
      181 tgccgttgct cttacaagag aatagcggct gatgtgattt gaaagcaccg agcttaaatc
      241 cgggttaaac ttgaaatttt tgttctgatc aaacattttt acatacctca ctaaaaaatt
      301 tattacatct gcttactctg tgtgtttcgg cagtgataat cgtttttata tcgtcaacgg
      361 cgtccttaag atcgtcattg ataacaacgt agtcatattc attgcggcat cgctcaattt
      421 ccctgatagc gattttcaag cggcggagaa catcgtcctc ctcctcactt ccgcggttgc
      481 gaagccgagt ttcaagaatc tccatgctcg gcggcataat gaatatgctg acggcgtcgg
      541 gatacatctt tttaatattc cctgcgccct gcacgtcgat tttcaaaata acaatatgtc
      601 cgccctcaag ccatttatcg acaggtccct tcggggtgcc gtagtagttg acgccgtaac
      661 gtgcatattc aagcagctcg ccgttttcca cagcatccaa aaaatcctct tcactgacaa
      721 acatataatc tattccgtcg atttcggaat ctctcggctt tctcgttgtc attgaaaccg
      781 attttgtgat atcaatatcc gatttaaaaa gctcggcaat gaccgtatct tttccggagc
      841 ctgccggacc cgaaagaata acaagcatac cgttattctt catcgtcctc atcctcctca
      901 taatccttgt cctcatcgag tctgttggca accgtctccg cctgaagata tgtcagaata
      961 acctgatcgc agtctgtaat aataaccgct ctcgttcttc tgccatgggt tgcgtcgata
     1021 agtgtcccct tctccttgca gtcctgaact atacgcttta tcggagccga ttcggggctg
     1081 acaatagcaa taattctgtc cgcattaacc atattaccga agccgatatt gataagcttc
     1141 atattttcac cccgttattc gatattttga acctgctcgc ggattttttc aacctctgac
     1201 ttaatttcaa gtacacgtct tgcaatttca acgtcatttg cctttgatcc gatcgtgtta
     1261 gcctcacggt tgttttcctg aacgaggaag tccatctttc tgccgacagc ctcggttgaa
     1321 ttgaagaaat ccttgagctg agaaatatgg cttctcaagc gtacagtctc ctcggcaacg
     1381 gcaattttgt ccgcataaat tgccgcttcg gtgagaagtc ttgcttcttc aacatggata
     1441 tctccgagaa gctcctgcat tctcgcaagc agcttttcgt tatattcctt gaccgtctcg
     1501 ggcgatcgtt tctcaacaaa ttcaacatat gaaagaataa gatcggctct tgaaacgata
     1561 tcatcacgga gcttttctcc ctcaacctga cgcatattga tgaatttctc aacagccttt
     1621 tcggcaactg ttttaacagc gttccagatc ttgtcctcgt ccgcctcggc cttgcggaca
     1681 acaagaacat cgttcattct cgcaagaagt ccggcattga tgttgttctc gattccgtag
     1741 gtctcggaaa gctcgttgaa agccttcaca tagcctgcgg caagggattt gttgacctca
     1801 acaacaacag tgtcgtcctc aaggttgtcc acctgaacat aacactcgat tttacctctt
     1861 gaaactttag actgaacaaa tgatttcagc ttttcgtcaa gaaaaccgta atttctcgga
     1921 actcttgatg aaaaatcaaa atatctgtga tttacgcttt ttatctcaac ggtaacgttc
     1981 attccgtcaa ccgtttcctg acaacggccg tagccggtca tacttttaac catactaaac
     2041 caacttcccg aattattttt tcttacagca gcttcctgca agcagctcgg ggatctcccc
     2101 ttcgagtatt ttttctttgt tttctttaaa accgagcatt ttagcaacgt ttacaccgct
     2161 tgcggccttg taatatgcaa tataagctcc tcggtttgcg ccgtagttaa gcgagcttct
     2221 tataaagccc tcgaccgttt catcgtcgct ctcgctcaca acgtcaataa tctccattgt
     2281 gtagccgttg agcctgaaaa cggaaaagcc gcactcagca ccgc
//
LOCUS       DAWBSW010000253         2296 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7487_length_2296_cov_1.761714, whole genome shotgun sequence.
ACCESSION   DAWBSW010000253 DAWBSW010000000
VERSION     DAWBSW010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7487_length_2296_cov_1.761714"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    862..871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2052..2061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          643 a          530 c          507 g          596 t
ORIGIN      
        1 aaattgcata taggagagat ttgcttcgtc cgtgtccgcc gtcacacctg tttttttaaa
       61 aaaatcgcca aatctttcac ggaaaagctg aggtctcgtc agctctcccc tctcaagctt
      121 tttccatttt tcatcgttga tttttgaata aagccgacgg ttctcctcag tcacggggat
      181 attatatttc tccatcagct tgtctattgc gaatttttcg gattttgaga agtcgaaaag
      241 cgtttcgtca aggtcaagaa gcagcgttgt gtatttcatt cagtcattcc tttcgctttg
      301 atatctttaa taattctact cttttccaag ccgaaaatca acaatttaaa caatctgtaa
      361 aaattttaac aatctttccc gtttcaggtt gaaaaataaa cgggcattat agtataatat
      421 cgtaagaaaa tattggaggc agataaacat ggaaattgat atgtcaaaca tcacttcacc
      481 cgagcttttg caagaagaag ttgaaaagat gaactccttc ggcgtccgtc tgaccggcac
      541 aaaggctcaa agaaacttta taaaatacct caaaagccgt atacacgaca tgggcattga
      601 aaccttctcc gacccgagcc gcttcatgcg ctgggaggag acaaacaaaa agcttgtaat
      661 taaagacttg gatgaggagt tagaggttcc tatttcttcg gcattcccat attcgggcag
      721 aactcccgcc gagggcataa cggcacagat gacgcttgtt agagaaaagc atgtcggcta
      781 tctcaacgca acagataaaa tcgccgttgt tgaggtcgat gagcttgact ttttgccaag
      841 tgaaattgct ttcaacgagc gnnnnnnnnn ncttccgagc cactataacg gacccgttgc
      901 aacggcattt gtcaacttcc cgtttctcaa aatggcgaag ctggcaggcg caaagggcgt
      961 tatctgcatt tggaaaggca taaatgacga ctgcatcgag ggtcagtatc ttccgttcat
     1021 tctcgactat cagggaattc ccgccgtttg ggtcaactca aagaacggca aaaaggtcat
     1081 tgaggaggcc gaaaagggtg catttgcgac gctcaccctt gaaggcataa ttgacgaata
     1141 tgcaaagaca gaatccttct ggtgtgtgct tgacggcgaa gaagagggcg aaagcgttat
     1201 catcaacacc cacaccgacg gtacaaactg cattgaagaa aacggtccta tcgcaatgct
     1261 tgccatgata aaatatctca aggataagcc tctcaagagg acgcatattt ttgtatttgt
     1321 cacaggtcat ttcagactgc cgaatttcaa gaaccttgac ggcggcggag ttcaggcaac
     1381 atcaaaatgg ctgaccgctc acagaagcca ctgggacggc aaggagggtc acctcaaggc
     1441 ggttgccgga ctttccgttg agcacctcgg ctgcaaaatg tggaaaaccg atgagaacgg
     1501 cgaatacagg cagaccggtg acgttgaaac cgaaatcgtc tacacaggca acaaggtcat
     1561 ggacaacatc tatttcaagt cgcttgagga tcgtaaaaag gtctatacac tcacgctcag
     1621 aggacataat cttcttcact tcggcgaggg acagccgctc ttcaactgcg ggattccaga
     1681 gattgctctg gttaccgctc ccgactgtct ttgtgttatc agcgataacc acgagatgga
     1741 taaattcgac gccgagctca tgtatgagca gacggtatcg ttcatcaaaa tggctctcat
     1801 gatcgacagc ctcaccgctc aggaaatagg cccctgcgac ggatactcgg ccattgcgac
     1861 aaagaaaatg gttgaggacg gcacagctaa aatcaaagag gttgccgcaa aggtcaagga
     1921 tattcttcct ttctgatttt aaggcagcac cgcacaattg catcaaaaaa gctccgaggt
     1981 caaaaacttc ggagcttaat ttttatttta attcaattaa atcaatacca gaggaagcct
     2041 aagagtacga tnnnnnnnnn natcatgatg atccactgcc accatgcgta gcttactgtt
     2101 accttacatt ccgccgctgc tttgccgtcg gtgcttttgg cagtaatcgt agccgtaccc
     2161 ttacccgtca tttttacgtt gccgctgtcg tcaacggtcg caaccttggt gttcgaagat
     2221 tcccacttga tctgctttgc gtcatagctt gactcaaaat caatcttttt agtctgcttg
     2281 tagtttgcac tgattg
//
LOCUS       DAWBSW010000254         2289 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7521_length_2289_cov_2.106535, whole genome shotgun sequence.
ACCESSION   DAWBSW010000254 DAWBSW010000000
VERSION     DAWBSW010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7521_length_2289_cov_2.106535"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          656 a          435 c          574 g          624 t
ORIGIN      
        1 gtataaaagc aaggagcgca agcgcggatt caataaaagc aggttgcttg ccacggagat
       61 atccaagctt tgcaatattc ctttagctga cgggcttctc gttaagcttt atgataccga
      121 ctgtcagcac gactgcaccg gtcttgaaag aacaggaaat ctaatcggag ttttcgatgt
      181 tgataaatca ttcgatattg tcgataaaac gattttattg atagatgatg ttaaaacatc
      241 cggcgcaact ctcaatgaat gcggaaaaat gttgtatttg aatgatgtaa aaatcgtaat
      301 ttgcttgact gctgcaatca gaaatagtaa aatagaagaa acaaatgatt gatacgaggt
      361 gttgattatg aaaaacggaa ttaaaatttt gatcgctgtt gttgcggttg tgctcgttct
      421 tgtcggcatt gttgccggag gccataattc acttgttgat gctcagactg ccgtcgaaac
      481 aaagcaggcg gatattcaga cgcagcttca gcgtcgtgcc gatcttatac caaactttgt
      541 ttcaaccgtt aagggctata caaaatatga gcaggagact ctcacagcgg ttacagaggc
      601 aagaagcaag gttggcaagg ccgctaatgc cgaggctctt gcacaggcaa gcgatgagct
      661 tgacagggct atttccgtct gggttaatgc tgttacggaa gcctatcctg acctcaaggc
      721 aaattcgaat tatgttgcgc ttcaggatga gcttgcaggc acggagaaca gaatagccgt
      781 tgcaagaaag gactataatg aagcggttca gagctataat gccgctatta aaaagttccc
      841 gaaaaatatt cttgccggaa tgttcggatt tgaaaaggcg gatttcttca aggctgacga
      901 taattcgcag acagttccta atgtggagtt ttaagttatg aaaagaatta tttcggtttt
      961 ctccgtaatt cttatattat ttattatatt ttctttttct gcttcggccg ctaaaattcc
     1021 caatgcaaca aaaaatttct tcgttaacga ttttgcaaat gtaatttcaa acgaggacga
     1081 agcgaaaatg cagcagcagg gtgaggcgct ctataaggcg tgcggcgctc aggttgttgt
     1141 tgctacggtc ggcaatacgg acggggagga tattgagtct tattcccttg agcttgcacg
     1201 ttcgtggggg atagggtcca agaataacaa tgacggaata ctgctccttc tttctgttga
     1261 tgacagaaag gtgcgtattg aggtaggcta cggtctcgag ggtgccttgc ccgacagcaa
     1321 aaccggaaga atcctcgata catacggtat tgagcacttt aagaaaaatg atttttcgac
     1381 aggtctcacg gctgtttatg attcgcttgt caatgaggta tatatcgaaa aaaaccttca
     1441 accggcggag gggtatactc ccatttccga agttaaagga tccgacaaag ataccgacat
     1501 cggctttatt gttgttgctg ttattattat tttcagcttt ttgatgtcat tcagaaagag
     1561 acggcgcggc aaatattact ccgattatga cgatacttat cacggcggct tcggcggagg
     1621 gttctcaggc ggaggatttt ccggaggaag cagcggcggc ggtttttcgg gtggcggagg
     1681 atcgtttggc ggcggcggtt cgtcaagagg cttttgagaa aaaacaaacc gcttaattat
     1741 tatttgatta ttttgaaatg aaaggagggc gtttattatg ctcggaacaa aaatcggctt
     1801 tgatctcggc acaaccacaa ttgcggctaa tgttgacggc aagggaatta aaataaacga
     1861 accctccgtt attgtttacg attgctttga agaaaaggtt aaagcaatcg gaaaagaagc
     1921 atataaaatg ctcgggaaaa ccccggatta tctgacggca attcgtccca taattgacgg
     1981 tgacatttac gattacggtg ctcttgaaca aaggctttgc tattttatcc aaaaaatatg
     2041 cggaaaccgt attcttaaac cgagtattat agcgtgcgtt ccgtcagaga ctaatgaaat
     2101 cgacaaaaag acggttacag atcttttgat atcctcggga gcggcgagag cctgtgtaat
     2161 tgaggaaccg ttggctgccg cactcggtgc aggggtcagc ataaagaatc ccgagggagt
     2221 cctgatcgtc gatatcggcg gcggaacaac ggacattgcc gttgtaacaa ggggctgtat
     2281 tgcaaactc
//
LOCUS       DAWBSW010000255         2288 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7526_length_2288_cov_2.621585, whole genome shotgun sequence.
ACCESSION   DAWBSW010000255 DAWBSW010000000
VERSION     DAWBSW010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7526_length_2288_cov_2.621585"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          699 a          410 c          471 g          708 t
ORIGIN      
        1 ctttgaacgt gtttatcaaa acagcataga aaatgcgccc ataacagcag gacatcccga
       61 tgacgaaaat ctatatttct atgtgacttc gtctcagaat attgcaacgg tttatatgaa
      121 cggcggcggg gtcaagtacc tgagctataa cagcaacggc tttgatgaaa ttaacccgtt
      181 tttgaaggga gacgcaaggt attcatctta tttgattgtt tccgataaga acaacatttc
      241 cgttgccgac ggctttttta tagaaaaaga gaactgccgt catatttata acggcagttg
      301 cgtggataaa tttgaaattg tcataagggg agatgtgaat cttgacggaa atctcgacgg
      361 aatggacgct gttgaagcgg cttgcgtcga gggcggcatg aatacggata agcttgcttt
      421 gatgcttgcc gatgcaaacg gcgacggata tgtaaacacc tcggatatca gcttgttgga
      481 aaaccttgga attgcgtcaa agaattaaaa atcgttgtct atcggatttt ctctcatttc
      541 gctgattttt ttcattcctt ctttataatc tgcggtttca cggttttcgg attcttccgt
      601 ttccgtttga gaggaaagaa tgttctcgac aattttattt tcattagcgt cgtcaacaat
      661 tatatgctct ttcatttgat ttacggcttc cataccctgt gaaacatcgg ctgtttcgct
      721 ttcaaagttt tcttcatcaa tcagattcag cggattaagt tcttcgttgg gcttttcgtg
      781 aggctcggca aggaaattat caattttttt atcgttgttt atgtcaatag tttcttttgg
      841 agtcagatat tcgttaagct ccataatatc ggcttgtgcg gactgagcct tgattttttt
      901 tctgacaacg gcggtgaaga cgagaataac aatcagtgtt atcacgaata gaacaatcga
      961 aacacggcac gacagaacaa aatcattgaa gatttttgcg ataagaatgt ttgcgatttc
     1021 cataactatg aaatataaaa aggcaaatat ctcggctccg ccgcaaagct ttgtcggccg
     1081 tgaccttgag ctgctttgaa ttcctttgaa atagaaaaag aaacgtgcaa acggtgccag
     1141 aaatatcctg ttatcgttct ttctcagatc gcggtcatat gtaaagccga aaagatagca
     1201 ggtcagaaaa gcaccgaaat taaatgccgt aaatattatg caataacata taatgagcaa
     1261 attgtaatgt cccataagaa tcttcctttg gttttatatg agttaagtta atatcgcaca
     1321 gttgaggtat acgaattgcc ttaattatac tatatattta attaaaattc aaatgctata
     1381 gcgcctaaaa tgtgtgaatt tttgttgcaa gcatcagatc ggcagcgata aattattaag
     1441 tccttgtcgg tttatttaat cacaaacagt tttatgatcg aaaacaaaaa taaaggagaa
     1501 tgaaaatgac agagcttgaa aaaatgcaaa gagaaaaatg tgcattgata agctcgcgaa
     1561 cggagtcgat ccgcagacgg ataatgagat gacggacgac cgcaggctta acgatcttta
     1621 tataagcagg tgccatcaca ttccgaaaga ggggagcttc aaagtcggag cagagtatga
     1681 gtatttctac atcattgacg gcatctatgt tgtagacgat aacggtgaca gttttgactt
     1741 taatgattat acctttctat ggcatttcac aaagctgtaa atgcaaaaga gctatcagat
     1801 tttttgaacg tctgatagct cttgttttga aatttcttaa tttaatgtct gcttttgctt
     1861 ttcataattt ttttaacaat tatccgtacg gttagggtca acaaaaatat tgcgagttga
     1921 ataaacgcca gtatcgttgc atagggcaat ccatagtcat acagctcacc acaatagatt
     1981 atacttccca ccaataattc gattactgaa atcggaatga atataaacaa ccacataggc
     2041 ttgatgttta agcacgacag caaaagtgca gaaaaagcac cgcctaaaag tgcagaaagg
     2101 gacacagcca ttgcgtcaga gggtgttgca ctcaacggca caccaatcat aaataatgtg
     2161 ttgaccaaaa cagccgctat atatatcagt atttgtgcag cttggagctt tttgttggag
     2221 gcttttattg tttcaacaag aatttcatct gcttttttgt ttttttgcat tgtgatgtgt
     2281 tcgcctat
//
LOCUS       DAWBSW010000256         2268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7609_length_2268_cov_2.233168, whole genome shotgun sequence.
ACCESSION   DAWBSW010000256 DAWBSW010000000
VERSION     DAWBSW010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7609_length_2268_cov_2.233168"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          626 a          468 c          523 g          651 t
ORIGIN      
        1 attcaacgta aagatccatg tttcgtgcat ttgaaataac ccatgagctg attttttcaa
       61 aaacaactgt agtgagaaga ataagcaaga atccgagaat ggcgcactca taatagcctg
      121 agccgatcgc aagacccata caggccgaag cccaaagacc tgcggcggtc gtcaagcctt
      181 tgatttcctg acgtccggta acgataatag ttccggctcc gaggaaaccg ataccgctga
      241 caacttttgc gctatatctt gaaacgtcaa cctgcttatc aagcattctg gcaatttctt
      301 cccataaatt tctcataata tatgtttcgt aatggctcaa aatcattgtc aaagctgcac
      361 cgagacaaac tatcatgtat gtacggcttc cggcggcacg gtgtttatgt ccgcgctcaa
      421 gtccgattag tccgccgaaa aaaacggcaa gaagaatttt gatcaccagc gccggcattg
      481 ttacgccgcg aatcggatca aaaaagggga taagtgaatc catttgcttt acctccgtta
      541 tgatttttgg ttctcataat aaataatatg ggcggacgtg aattttgttc cacgcccgct
      601 ggtcgaatat gatgttaaat tatactacaa ataatcagaa tagtcaagga tttttgtctg
      661 aattattgcc aaataaatgt cagaattatt gtaaaataag gtgcaagaat actaatgagg
      721 tgattttatg aaaagtgaaa aagaaaaagt atatttgccg attgatgagc cggttatgaa
      781 tgaccccgaa aactgtgccg atatccttca taagtacgga acctatgaga tacagcccac
      841 tgcagcgacg gaaaacaaat atccaaagat tgctcagggt cttccgaaaa agaaaagcag
      901 aagaaatgat aattaaaaat atataattat ggggtcgtaa aaatcgaccc cgtatttttg
      961 ttacatttaa cttattttac atagtaccgg ggctatttac atatcggagg aaatgtatta
     1021 taatttaata aaaatatgaa ttatcggaaa ggggtagttc tatgcaaaac ttagcggctt
     1081 catctgcaaa tgttctcgaa cagcttaaca gtacgcccat gtatttgatt tgcggcggaa
     1141 ttgttgcgtt cgttgcgatt gtttgcctca tcttccttgt gcgtgcctat aaagcgggag
     1201 tcgctctcgg aatggacacc acaaaaatga aaagagttat cgtttcaagc gcaacatttt
     1261 cggttttgcc gagcgtcgga attcttctcg gagttattgc tctttcgggc agtctcggaa
     1321 ttccgtggcc atggcttcgt ctttcggtta tcggcgccct gcactatgaa acacaggtcg
     1381 ctcaggcggc agccgagcag gtcggaatgt ctaagctttc tgctgcggaa atgaccccga
     1441 aatcctttgc cacaatagct ttgctgatga gcgtttgcat catgtgggga atgattcttt
     1501 cgatattctt taacaaaaaa tattctaaaa agctcggcac agacggcgga agctcaaagg
     1561 caggcttcgg cgacaaggct atgacggcaa tgttcatcgg tcttgtcagc gcctatatcg
     1621 gcagttatct cggctcattt gtttcaggca atggtctttt caccttcacg ggtgactggc
     1681 ttccgttggc tgttgcggct atttccgcat tggttatggc tttgtttgtg ttcttgggcg
     1741 agaagaaaaa acttgcctgg gtggataatt tttcaattgc aggcagtatg attgtcggaa
     1801 tggcttcggc tgttgttatc ggactgctga ttaaataagg aggggccatg atggataaaa
     1861 ataacaatgc tgtatgggaa aaatacagta aaacaactca ttttatcgga agatttgtca
     1921 gcgcaattac cgttattatg ctcgtcggcg caccgttttt aatcggaaaa tatctcggtg
     1981 cttttcccga cattggcgca gtgggcaagg cattcttctc cgtcggactt gtttggacgg
     2041 tttcgagcgt tgcggaattt ctcatctaca cccctatgct cggctcgggc ggcggttatc
     2101 ttgcatttat taccggtaat cttataaaca tgaaaatccc ctgtgccgtt acggcgagag
     2161 acatggtcgg cgtaaagagc ggcactaagg aaaatgaaat aatttcaact ctttcgattg
     2221 caacctcgtc ccttgtgacg attcttgtgc ttgcggtagg tgtgctcg
//
LOCUS       DAWBSW010000257         2249 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7692_length_2249_cov_2.741112, whole genome shotgun sequence.
ACCESSION   DAWBSW010000257 DAWBSW010000000
VERSION     DAWBSW010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_2249_cov_2.741112"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1449..1458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1972..1981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          587 a          492 c          559 g          591 t
ORIGIN      
        1 gcgttaagcc gccgtacatt cttttatgca accataatgc attctttgat ttttacatta
       61 tgagtgccgc aatcagcccg aacaggggta tttttccggc tgccgttgat gactatatcg
      121 gcagagaatg gggcttgagg agactcggcg ctatcccgaa aagaaaatac actgcggata
      181 tctcgcttct gcgtgtctgc cgcaaggctt tgaaagaaaa acagattttc ggaatttatg
      241 ccgaggcaag gtattccctt tgcggagtta ccgaggttat tcccgatgct gtcggacagc
      301 tcgtcaagca tcagggtgtg cccgttgtaa cgctcacctg ccgaggacat catatctatg
      361 acccgttctg gggcaatcac agaaagcgct gggtgcatcc ggttcaggct gatatggttc
      421 ttgcctatac gcccgaggag ctgaaaaagg cgagcgcaga ggaaatcagc gagaagatta
      481 aaaatcttct ttataatgac gatttcagat ggcagagtga aaatcacgtt aaggtcagct
      541 acaagaagcg tgccgaggga ctgcacaagg ttctctatca gtgcccgcat tgcatgaccg
      601 agttcaaaat gaactcgaag aacgacaagg ttttctgcga gcattgcgga aagtcatgga
      661 cgctcaatta ctacggcgag cttgaagccg acgacggtaa gacggagttc aaatttgcca
      721 cagattggta caagtgggag cgtgagcagg tcaagaaaga ggttcttgac ggaagctacc
      781 gcttcgagag tcaggtcgac gtcaacgatc tgccgaattc aaagggattt gttcacatgg
      841 gtagaggtac gcttgttcac gatatgacgg gcttccatct taaaggtgtc agggattatg
      901 acggcgaacc gttcgagatg aacattgatg ccgcaggtca gtatgccgtt catgttgaat
      961 ataattacag attcggaaat caccgagact gtatcgacct taacacattg gaggacacat
     1021 ggtatgtctt ccccgagcat tgtgattttt cggtcacaaa gatttctctg gcgaccgagg
     1081 aaatatttaa cgaaatatgg cgtcagcgca acgagaagcg caaaaccgct aaagctgagg
     1141 agtaaaaaat atggatatca gaaacgacct tttggagatt attaacgagt ataccgacgg
     1201 cagaatcaca tccgttgagc cggacgacgt tcttacctcg gatctcggac tcaattcttt
     1261 cgagcttttc gatctcatct gcatgatcga ggagaagtac gacataacga tccctgacag
     1321 agtcctcccg actcttgtaa ccgttaagga cgttgtttat tatctcgagg agaacacata
     1381 agcaaacaga aacaagagag cctgccggat tgccgacagg cttattttta aacaaaggaa
     1441 acgagaatnn nnnnnnnnaa aataaaaccg acggtacggt tatcatcgga cggaaaatcg
     1501 ctcggttttt cttttttaaa tgattgaata aatgcaaata gtttatagcg tatacaaatt
     1561 tccacggaac gattttaccg taccgtggag ttttttattt cggctcgctt gctatgggct
     1621 ggagctgttc gcccttgagt gccgcttcaa cggcggcagg tataagcgtg aagtcggcgg
     1681 caagcgaacg cggcttcttt tcgtgatcgt cctgtctgaa cgggacaagc catatatttc
     1741 tgtaattgag caaggctcct atgtttttgg ccgcacatgc aagaccgtca tttgtcgaaa
     1801 cggcgatgac aacgggtctg ccgtttctca aatgcgattt tgccgccata gttaccgatg
     1861 tatcggttat cccgttggca agcttggcgg cggtgttgcc cgtgcaggga gcaataacga
     1921 gaatatcaag catttttttc ggccctatcg gctcgacctg cggaattgtg tnnnnnnnnn
     1981 ngtatttcat gcttgcatat ggtttctatt ttatatctga attcctcggc tttgccgaaa
     2041 cgggtgtcgg tctcgtatgc gatttccgac attatcggaa ctatatcgta gttcagcttt
     2101 ttaagcattt caatctgcgg aataacttct ttgaaggtgc agaacgagcc gcacatggca
     2161 aagcccacaa ctttattcat tcttatcctc ctttatcatc tcaatgaccg tatcggcaat
     2221 gtttgcgcct gcgcttgtcg gtgtaaagc
//
LOCUS       DAWBSW010000258         2243 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7721_length_2243_cov_4.857861, whole genome shotgun sequence.
ACCESSION   DAWBSW010000258 DAWBSW010000000
VERSION     DAWBSW010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7721_length_2243_cov_4.857861"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    690..699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          573 c          442 g          726 t
ORIGIN      
        1 caactacagt gccgccgttt atagttatcg ttccgccttt tcccgaagag cctcccccga
       61 tacctgcgct gtaaccgtcg gtgccgcccg ttgcatatat tgttcccgaa ttgatagtca
      121 gtgttccgtt tgattttccg cttcctccgc cgatacctgc ctccagctcg ctttcggcat
      181 agagtatgcc gtcaccgttt atcactgctg aagcctttac ggcaatgtcc agtccggctc
      241 tgtacgttcc tgattttatg tgattttcgc cgctaacggt tatcaccgca cttgcggtct
      301 ttaagacaca cagagcataa tcgttttcgc cctttcttgc aatattgatg ttcttaatct
      361 caacattctg ctcaccggaa gtaattgata tgcttctttc gagtgctttc tgcggatttt
      421 gttgggtaac ggtataaccg acggcattca ctttcgaaac aagctttccg ttttcatcat
      481 agccagaaac gccgtccgaa ccgattttaa catctccgta atcaagataa aacacagttc
      541 gatcttccac tgcctttaca gaagagttta acggcagaac tgctaagcac aatatcaata
      601 tacacaggat aacctttttc aaaatcaatc accgtccctt ttaaaagcaa acggcacctt
      661 tgaggggtgc cgttttgtat tagtctatan nnnnnnnnna ggacttgaac ctgcggcatc
      721 ttggtcccaa accaagcact ctaccaaact gagttacgcc tcgacgttgc aaacacaacg
      781 ttgttattat atcataaaaa cggcttttgt caatatcaat acggcaaaaa gcgcttcttt
      841 tttaaaagca aacggcacct ttgaggggtg ccgttttgta ttagtctata ctgaaaatgt
      901 ttccgagtgc ttcgcccgcc gtctcgaacc acgggtcttt gttctcgtca attatcgggt
      961 gaaaaccgcc tgcgctgact ccatcatcca tgatattctc aactatcgaa aacttttcaa
     1021 tctttatctc aggtttttcg tatctcattt ttttgccccc tgtttcgtaa agtatgaaat
     1081 tatttcgttg ataatttcta tcagcttcga gaataccgtc ttgagtattt caagcggact
     1141 cttctctgtc ggctcgtcct ctatcgtcgg tgcttccgcg tcgctgttga gatctgtcag
     1201 atcgtagctt tctgcaccga gagttactat ttcatcgcct gccgatgagg ttcccgctgt
     1261 cacgtcaaac tgtctcaccg tgccgtaatg aacttcgtta tttgcatcaa ttacatacgt
     1321 ccttgcccat accgtgtttg caccctgaag atcaacgctg acctcaagca agcccgtaag
     1381 tgcatttgcc gattttaaag ccgccttttt ctcaacattg tcgttgtctg ttgtgagcat
     1441 tcctgcattt gcctcttccg ttgtgtaaat cacgccgtgt cccttaattg attcgcatga
     1501 tgatgctacg ctgtgctcgg caagcagtga atagctgccg ttgtcgttaa gtcggcaatc
     1561 aacaactctt gatgcaatta cagcttgatt tctcattgcg taatactcct gctgagttgc
     1621 atataccgga gtaaacttac tgctctttgt ctggaagtat ctgtatgttg tgtacgttcc
     1681 gaccttctcg cctgtctccg catcaagcca atatacgaag tacatcttct gtccgttctc
     1741 cgttatcgtc atcggagcct tgatcgttgc gatgtagtag ttctttgact tcgcctcatt
     1801 gtataccggc ggatttgccg ctgcatactt ctcacattcg gatttgatat atgcattatt
     1861 tgctgccgaa tatgaattgc aactgaattc aaccgcatat gccttggcag tcgttgtgta
     1921 gctgtaaccg cactctgaac acgtatatga atatccgtta tcggtcttta catatacaag
     1981 cttatgcttt tcgggtgctt tcttgtagcc gcagtcatta catacgtact gatgctgctt
     2041 tccgtcaaga atatcgcatc tgtaatcatg cgctgtcttg ctgtactgtg cataatatgc
     2101 gatgtcgtcc tttaccgccg aaatctccgg agtccagccg ctgaacgtgt actcgtaatt
     2161 gtcgtccttt gactttgtcg gcgtagctcc gttatatgtc ggtgtggtgt tgtattccca
     2221 cttgctctcc tgaagaacat cat
//
LOCUS       DAWBSW010000259         2231 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7772_length_2231_cov_2.233456, whole genome shotgun sequence.
ACCESSION   DAWBSW010000259 DAWBSW010000000
VERSION     DAWBSW010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7772_length_2231_cov_2.233456"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1796..1805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          656 a          461 c          412 g          682 t
ORIGIN      
        1 cgcagatctc aaccttgttt ttatattgca tcaaaagacc tcctcacagt aaaattttta
       61 ccaagaggag gtttagttat tcagttaata tctgtatctc aaaatgtttt taataaacgc
      121 aaatgtttcc gcaataaaga aatccggtga agaaacgagt gcataaagca tacttttaat
      181 cattttatta tacatgtgat ttcgtttata tgcaacggac ataaccatgt tatgtctgaa
      241 tttaacatag cgaatttctt ttttgctcaa gcggtcaaaa tattgctttt tgaactcata
      301 gagcatattt tcaccgtcaa ttttgttctt tccctgggaa attcctccgt cgttatgttt
      361 atataccttg acgtcgcagg aatcaaagta tcgaatttta agcccggatt caatcgaaat
      421 aagcatcagg aaaaagtcct gacccatttt tgcgtccttg aaaccgccga tttctttcag
      481 tttatctgct ttgaacataa agcttggcgt gccgctgaga tttttcgtca gatggtagcg
      541 caaaagagtc tcattgtcat aagacctgat acctttatga tctctgaaat cgcaaacctt
      601 gccatcgaga cgatacataa tcatatttga aaaagaaagg tcacagtcgt ttgaaagcat
      661 aaatttcagt tgcttctcaa ccttgccggg catatattcg tcatcgtcgt nnnnnnnnnn
      721 ggaaagttat gtattcgccg cgggaagcgt ttataccgac atttcttgaa agagagccgc
      781 cgagattttt ttcgtttcta atataaataa ctctgtcgtc gttaagcttt tctatataat
      841 tctttatttc gtcacggtaa gggaaatcat taggactgtc gtctacaaca atgatttcga
      901 tattatcata ggtctgattt aaaacggact gtaaggcatt gcttacataa tccagttttc
      961 ttttgtaagt cggtattata accgaaacca agcaacccat caatttatct tccttcactc
     1021 aataacttca aaactttaat aatttattat acaaaaactc agtaaaattg tcaacgccta
     1081 gagaaaaatc gttatgaaaa aattgtttta agccactctg ccgaacgttc gagccaataa
     1141 gaattcggtc tacaggcttc tctgcccgtt tgagtggaac atacgacatc ggttccgagc
     1201 gaaaagccgt ggtcgccgtc gttaaaaatg tgcatttcaa atcctatgcc gtttttatca
     1261 agagcctcgg catatttaag cgaattgatt atcggaacac agccgtcatt cgatgccgca
     1321 aaaataaaag tcggcggtgt ttttgaagta acatatttat caacggactc aacaggcttt
     1381 gcgagaatag gacttgtcga atcaagaata caaggatagc cgagaataca agcgtccggc
     1441 ttatcctcag acattgttga cagtgccgca gcgagatgtc cgcctgccga aaagcctatt
     1501 accgcaattt ttcggggatc cgtgtgaaat ttttctgcat tttttctgat aaaagccata
     1561 gccttttgtg cgtcattcaa cggtgtcgga aatgccgcct tttcgttaag ggaatatcta
     1621 agaacaaacg cattgaagcc ctttgcaaga taagcaaggg caacgggttc cgcttctctg
     1681 tcagaacaga acttataagc tccgccgggt aaaacaagaa ttgccggctt tgttttcatg
     1741 ttcttcattt cttctgaaat atcggaaata tatgaggtta aagaaacctc cgaatnnnnn
     1801 nnnnngtaat tttaaaaatt tctgaaatca tgcttactcc tcaaaatcat tcatatcggc
     1861 ataggaacgg aaaacatatc cgttttcttc aagcaccttt atatcatcga gaagaacccc
     1921 gagtccgttt gcaacgcaaa gctccggaga ctcggcgcat ttgacattga aaccgaattt
     1981 agtgcgaaaa taatcatcaa tgcctcgaat atttgctcct ccggtaatga tcattccgtt
     2041 ctttgcgata tccgcgacaa gctccggctg agtttcggaa agcaataatt tgatgctttc
     2101 gacaattttc tcaagatgct ctcttatgca aagaaaaata tccgtcgatg taagctcaat
     2161 gcttttcgga agctttgtta tataatcctt gcctaccgca cgcacggcaa gctcagcgtc
     2221 aaggaatttt g
//
LOCUS       DAWBSW010000260         2224 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7804_length_2224_cov_2.725219, whole genome shotgun sequence.
ACCESSION   DAWBSW010000260 DAWBSW010000000
VERSION     DAWBSW010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7804_length_2224_cov_2.725219"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          506 a          578 c          468 g          672 t
ORIGIN      
        1 cgcaagagcc ttgagcgttc ctctcgcctt gtccgtagta gcatagatac gctttgccgc
       61 ttcctctgcg ccgtacttgc tctcaagcat ctctctgaaa atcctgaaag caaccgccgg
      121 ctccgtggtt gtgcccgatt ttgaaataac attgacggaa aaatctctgt ctccgattat
      181 ttcaacaagc tcgttgattg aagccgagct gatcgtatta cctgcgaaat agatttccgg
      241 tgcgccgtgt ctgagcagat tatagttctg cgacttgatg aactcgatcg ccgctctcgc
      301 tccgaggtac gaaccgccta ttccgattac tacaagaacc tcagaatcct ttctgatttt
      361 ctcggcagat gctttgattc ttgcgaattc ttccttatca taattttccg gaaggctgag
      421 ccatccgagg aagtcatttc ctttacccgt tccgttgtga agctcgtcat gtgcggcttc
      481 aacctccgat ctgatgtcgg catatgcgtt ttctttgagg aacggagtta catattttgt
      541 gtttaacttt acagacatta tctgtatgcc tccgatttac ttttcttatt agttggttat
      601 attttaccct atttaacttg tttttgcaag tggtatttag cattttttac gcagattgca
      661 taaaaaatac acactcagtt cacactaaca gactgctgcg gcttgcaaat tgagcatatc
      721 atgcggataa acaccgtttt gaagctgagc ttttcgacat aatgcggcgc agtcagctta
      781 tactctccga gcttttttcc gtccagcttg accgtcaccg tacccaaggt ctgattggac
      841 tcaacgggag cctcaactgc ggcggcaagg tcaatttcct gagtgatgtt cttttgcttc
      901 cccttcggaa taaggaagcc gaagccgccc gatatttgcg gcgtgagtgt tttctcctcg
      961 cccttgatga tattcacgtt tgtaatcagc gatttgtcga tttcgggagt taccgtttca
     1021 tagtttgcaa agccccagtt gagcatcgcc tttgcgccct caaagcgatc cttgctgtta
     1081 tccgcaccca tcacaacggc tattagactt gtaccgtttc tcgttgccgt cgctgaaatg
     1141 cagcagcctg cctttgacgt tgtacctgtt ttcagacccg ttgcgccctc ataaaaccta
     1201 attaacttgt ttgtgttgac aagctctgtc ttgccgtcgc gaagtgagtc catccagatt
     1261 tttgtgtact gctgaataaa ctgatgcttc atcagctccc ttgacatcag cgcaatgtca
     1321 tacgccgttg tcaggtgagt ggccgaggtg tcgtcaagac ccgtgcagtt gtcaaaatgt
     1381 gtatttttca taccaagctc ctgtgctcgg tcgttcatca tcgttacaaa cgcttcatcg
     1441 cttccggcta tatattctcc gagggctgtg caggcatcat ttgcgctgta aacggctgtt
     1501 gccttcagca attcgtcaac ggtcattgtt tcgccctctt tgagccagat ttgcgaaccg
     1561 cccttttttg acgcattagt gctcgttgtc accgcgtcgg tcagccttat cgtattggaa
     1621 tctatcgcct cggcaaccaa cagcattgac attatctttg tcaccgaagc aggatagagc
     1681 ttttcgtttt cattcattcg cataagtacc gttcccgttg actgatccat cagaactgcc
     1741 gattttgctt tcacgtcaac cggcatctgc gactcatttt ccgccgaggc aaatacacct
     1801 aatattccgc acatcataat aacgcttaat aggattgcag ttgctttctt cataataagc
     1861 ctccctttca ctgctgtaca caataaatat atgccccgct aacggcaaat aaaacttgca
     1921 ctgcacaccg ttttgcttta taatattatt ttagattacc aaacggagct gatgatatga
     1981 aaattgcatt ttacaccctg ggctgtaagg tcaaccaata tgaatcccag gctatgtccg
     2041 aaaaagcggc ggcgaacggc tatgaaaccg tcactgccga cgaagaagcg gacgtttatg
     2101 ttgtcaactc ctgcactgtt accgccgaga gcgaccgtaa gacacgtcag gcggtcagaa
     2161 aattcaagcg caaccacccc gaaagcatag tcgttctcac gggctgtatg cctcaggctt
     2221 ttcc
//
LOCUS       DAWBSW010000261         2219 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_7829_length_2219_cov_4.109519, whole genome shotgun sequence.
ACCESSION   DAWBSW010000261 DAWBSW010000000
VERSION     DAWBSW010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_2219_cov_4.109519"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1234..1243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          588 a          520 c          484 g          617 t
ORIGIN      
        1 gctcaagaga tttgcctgtg caaaagccga gacccaccgc atcaagggtg aagcattcgt
       61 aatgctcgtc atccgtgaag ttgaaaaatc ctattgcgag atcgccgttt tcaagatgtc
      121 ttacaataac gggcatatgc tcgggatacc agctgtagat cggttcgtcg ccctttctct
      181 cgttatttac atttctgttt ccgttaacaa aataaggctg tctgtatgcc gcatcctggt
      241 caattctgat aagctcctta ttcttgagga tagccatagc ctcctcgtcg gcttctctga
      301 ggtcgcagcc tataataagc ggagagccca tcattgccca aaaagcaaag tgtgttttgt
      361 attcctcagt cgtacagccc ttgagcccga catggccctt acccttcatt ccgacaacga
      421 gcatatccat atcgttaaag cagccgcaac cattatactc cgcaaccttg tactgcgaaa
      481 gtgcaagctc cttgatcgac tcccatgtat caaaaatgtc gcctgtcgaa cgccatgtgt
      541 gtgctccggt ttccttgatc catgtctttg tgttttcact accccacgaa catgcggcga
      601 aaagaatgtc cctgccgcaa ttggcaagag cgagacccat tctcttatag agcacatgac
      661 cgtaggtcgt gaacggatga taacaatagt catatttgag gtaatcgacg ccccattcgg
      721 caaagcattt cgcatcaatg aattcatggt caaggcttcc gggataagcg gcgcaggtca
      781 tcgttccggc acaggaatac atgccgaact tcaatccctt tgaatgaatg tagtcggaaa
      841 catacttcat tccgtgaggg aacttttccg gatcgggaac gatttcattt ttctcgtttc
      901 tttctctgag gctccagcag tcgtcgatga tcacatattc atatccgcag tccttgagtc
      961 cgctctcaac aataaagtct gcggtttcca taactacttt ttcgtttatg ttctcggcaa
     1021 atgtgttcca tgagttccag cccatcggtg gtctgttaat taccataata tttagctcct
     1081 ttttgtttgg tagaaaaatt atatcaatta taaaagttgc tgtcaaccga aagtaaaaat
     1141 tattcggtca caaaaaagtc taaatgaaga tctggttttt taaattttct tatttttcta
     1201 tatttgcatt aacctgattg cattatttga tacnnnnnnn nnnatatatg tcaaaaggag
     1261 gaatttttat gaaagaatat attatcaggg agacggatga ttttctatcg ctttcgaccc
     1321 tttttcacga gagcggaatg ggtgttaata tcgaagaacg tatgccggac agagtaatca
     1381 agatgtggcg tatggataat gccaggacag gagaactcat ggcggcagta actcttgaga
     1441 ttcgtgacgg cgcttattct ttgggtgaca tcgccgtgcg cgataccgtg cagtccaagg
     1501 gctacggaag aatcctgcag tctatcgttt tcgacgaagc gaaaacgctc ggcgtcaaag
     1561 aaatctgggc ctgcgccaag gagcccgaat attatattca ctgtggctgg gaaagaatgg
     1621 attgggagga ctctcctaat attgccgttt attgctcaac ctgcgacaag cgcggcacca
     1681 cctgtcatcc ccagctgatg aaatacacat ttaaataatt tttacacatt aaagagcttg
     1741 accgtcttcc gtatgtcaag cttttttcag taaatcaaaa ccgccggttc aaccggaggt
     1801 ttgctccacc cctaaaaggg gcttctgatt tatagcaatg cattacactc tcagcagccg
     1861 ctcacgtgca ataatgtatc aaaaataccc ttcttttttt tggcgcctca aacagagatt
     1921 cccctgacag gggaaatgtc acgaagtgac aaaagggtga ctgcgaggta cgagtagctc
     1981 gaacctgtga ctttaaatat gttgtcggca actgccgttg cctcattgta tcgcaacgcc
     2041 aaaacaattc accggattgt tttgtctgcg acagataaca gccgcgttcc gttactgcaa
     2101 tacggtgtcg caggcgagag aactgcgcag aggtgccgac agaaacaaaa agaccgcaca
     2161 tcgtgcgatc tttttgtttg gcgcctcaaa cagagattcc cctgacaggg gaaatgtca
//
LOCUS       DAWBSW010000262         2170 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8070_length_2170_cov_2.713948, whole genome shotgun sequence.
ACCESSION   DAWBSW010000262 DAWBSW010000000
VERSION     DAWBSW010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8070_length_2170_cov_2.713948"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          570 a          461 c          471 g          668 t
ORIGIN      
        1 ttttcaaaag agagcaaaaa aagaaggacg gtttaaaccg tccttcaaaa accaatatga
       61 tttatttatt ttgtaagcaa tgaatcgaga acttcaagaa ttgtgatgaa gaagctaacg
      121 atgaggtgac cctgagcctt gccccaaacc ttaagtcttt cctgccatgt aggaacataa
      181 ggtgcatgat agaagcaagc tgtatcaaga acgatttcat cattctcgga ttcgttaagg
      241 aaaatacctg tgaagttgcc ttctgcatct gtatagccta cagagctgcc cgatgcatta
      301 ggatctgcaa cacaaccgtt cggattgcct tcgtcatctt ctgagtaaag gtcaaaatac
      361 gggaagaata cgattcttcc gaagtccttg ttgtactcct cgggaacatc ctcggttgta
      421 acaacatcaa tatagccgat aacgatacct tcgtcagatg ttgagaagaa cttgttgaaa
      481 tcagccttct tgatcttgaa agccaatgaa ccgtccatgc cgttttcaag atctacctgg
      541 ttgacaacaa gctgatcgtc agcagcaatt ttatcagaga atctgatgtt cttaatctca
      601 acaatatcct tctcgcttgt tgagaagctg atgcctgaga atccgaaata gaaagtttct
      661 gcgttttcag gaaccttctc aagataatct gacttcatga tgattgggag tgaatactct
      721 tttgagggct caatgtatgt attctggatt gagaaaacaa ccttgcctgc ctgcttaggt
      781 gtatctttag gcataccctc gtttacgttt tcgccttcgg caaatgctgc aacgccaagc
      841 gtgaagagca tgataaccga gagaagaatt gctaagaatt ttttcatgaa aaaatctctc
      901 ctttttgttt tttgcatttt catgcttttt attttgcgaa gggaacactt cgctttttta
      961 ttataatatc acgaaaaaga tttttatagt tcttttttag aaaaatattc actttttcat
     1021 aaaaatattt taataatttt tttgttttag tatagtttgt ttctttactc acaacatgtt
     1081 gtgttaattg cagacctcct gcatgatctc gttcacgacc tcgccggcaa ccgagagacc
     1141 cgatccggat ttttcaacaa cgcagataac ggcgtacggt gcgtcctcac gctgagaaaa
     1201 gccgacaaac catgcgtgtg agccgccctc aacctctgcg gtgcccgtct ttccgcacat
     1261 cttgagcccg gggaaggtgg aatcggaata ataattcaca acgtttgaac gaagaagctt
     1321 tttcatctgt gccgctatct cgggagttat gtttacggtt ttgttcacgg cggtttttcc
     1381 gataacgttg atcttgccgt cgggggagac catgttttga acaaaataag gctttgttgc
     1441 aacgccgttg tttgcaattg ctcccattat tgtgagcata tgatagggat ttaccattgt
     1501 ggtgtactga cctatacctg cccagccaag gtcggttttt gtggttttac tgacgtcaaa
     1561 gcggcttgcg gaaacggtta tctgatccat gtcctctttg gtgttgaagc cgagctctcc
     1621 tgctgtctgc atcagctttg attgaccgag ctgaattgca atctgtgcaa aagcggagtt
     1681 acaggaatgg ttaagcgctt cctcaaaatc gacggtgccg tgaacgccgc tgcatatgat
     1741 tttgccgtca cccgttgcat attcgcccgt gcactcaaag gtctgtgaat atatgtcggg
     1801 aatattttca atcgcactga tagcggtaac gactttaaat atggagccgg gggtgtacag
     1861 tcccgaaaca aagcgattga ggtaaacgcc ctcatattca tcggaatttt cgtcaatatc
     1921 gtccggcttg ttctgaggat catatgaagg tgttgatacc atgcagacca tagcgccggt
     1981 tttgtaattg taaacggcta tggttccttt cctgccgtcc attgcttcgt aagcggtttt
     2041 acatatatcg gcgtcgaggg tgagatttat gtcattacct ctgttatatt ttttcaggtt
     2101 gtaaacaccg ttcacaaagt cgtagcccga aagctttgaa ctgtaaacat actgaactcc
     2161 ggttgcgatg
//
LOCUS       DAWBSW010000263         2166 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8081_length_2166_cov_2.909048, whole genome shotgun sequence.
ACCESSION   DAWBSW010000263 DAWBSW010000000
VERSION     DAWBSW010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8081_length_2166_cov_2.909048"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          618 a          430 c          464 g          654 t
ORIGIN      
        1 gttcgatcac tggtatgaaa agcaccacgt cagagactcg aaggaggttg ctaagctttt
       61 caaggacatg atcgactgcg gagcatcgct gaatttttat atgttccacg gaggaacaaa
      121 tttcggctat atgaacggcg caaattacta cgataaatat gagcctacaa taacaagcta
      181 tgattataat gcactgcttt ccgaggccgg cgaccttact ccggcttatt ttgaagtcag
      241 aaaaataata gaagaacgtt tcggcagcct tcctgagctg acggttaaaa attccgaaaa
      301 agccgcttac ggtaagcttg aattagacga gagatgcagt atttttgatg cggcgaagct
      361 tcttgcaaag cctgttcact cagccgcacc tcagttcatg gaggatatcg gtcagtatta
      421 cggcttcacg ctttattcaa caacagttga cggtccgaga gatgaggcag aaattaagtt
      481 tgattcggtc catgacagag ctgttgtttt cattgacgga aaatataggg gctattatga
      541 gcgcacaaga gaaggcgagc ctgtcagctt ttcgctcgaa aaaggcgaga attgccgtat
      601 agacatactt tgcgagaata tgggcagagt taactacgga ccgaaaataa tggacagaaa
      661 gggcgtcaag ggcgtcagat tcaatctcca atatcatttc ggatgggata tgtatccgct
      721 gccgcttgat aacatttcat tgcttgaata caagcatgaa agcggagatg taaaaaccgc
      781 ctcgttcctt aaaggcacgc tcaaaattga gggagaaccg cacgacactt ttatgcgcct
      841 tgacggcttc acaaagggca ttgtatttgt caacggcttc aatatcggac gctattataa
      901 tccggcaggt ccgcagaaaa cgctttatgt tcctgctccg atgctcaaaa aaggtgataa
      961 tgaaatcgtt gtttttgaaa ccgatcactc ggacagaagc tttgtaacat tccttgataa
     1021 acccgattta ggataaacaa aaccgcctga aaaattgatt ttttaccgca gatcatatga
     1081 tttgcggtat ttttttattc tattcggcat agcctttaca aaggagttga caagctatgg
     1141 ctgtaatcgg aacaaagaaa acacttaatc ttcacaaata cagtataaaa gtgaatgatt
     1201 ttctttcaaa ttggaaaata ataatccctg taatatttac gtttgcggga atcatttccg
     1261 gatgtgtcgg cggaaaaggg gagggtaagc tctttttgag aataaccgac tactttacat
     1321 cggttatttt aagcagagat ataacctcag tcgggtcgga gtttttaata tatttaatct
     1381 ttcctattgt atttttgatt attatatttt ttttcggact ttctgtgttc ggttctctct
     1441 taacaaacgc aatgccgctt atttacggct acattgtcgg ttgtatctct tattttctat
     1501 acaccgaata cacgctaaag ggtctcggct actgtattat aatgattttt ccttacggaa
     1561 cgctatgcct tttgtcaatt gtactatgct gccgtgagag cattaacatg tccgaataca
     1621 ttatgaaaag catctcaaaa acgacaaaat tcctgaatta cggatttgcc gtttactaca
     1681 aatctttttt caggaattta atatttatca ttgccgcatc ggcgatcaag acaatacttg
     1741 aatatttatt cggagggctt ttttcctttt aacttattga gtgggtttga cgaaaccgca
     1801 tctttaagct gagagttaac aatatgggta tatatctccg tcgttgcaag gttttcgtga
     1861 ccgagaatgt ctttaataag aagaacatca acgccgcctt gctgatacat cagcgttgcg
     1921 gcagtgtgac ggagcttatg agttgataag ccacggtcgc ccagaccgca tttttcaata
     1981 tatttttcaa cgatatgctg aacagacttc ggactgattc ggcattttct gccgctgaga
     2041 aaaagtgcgt ttttgtcctt tacgccgtca acagggcgaa ccttcatgta tgcatttacg
     2101 gcattgacgc acatttcgtt aagataactt gtgcggtctt tgttaccttt tcccgtcacg
     2161 gtcatg
//
LOCUS       DAWBSW010000264         2149 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8159_length_2149_cov_2.117956, whole genome shotgun sequence.
ACCESSION   DAWBSW010000264 DAWBSW010000000
VERSION     DAWBSW010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_2149_cov_2.117956"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1904..1913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          630 a          504 c          468 g          537 t
ORIGIN      
        1 acggtttata aaatgatggt caatgaaatc tgaggagcga taactatgtt taaagaagat
       61 gcggaaaaac aaatcaaaga ggcctatccc tacaggcttg agcttcatgc acatacctcc
      121 cctgccagcc gttgctcgca ggtaaagccc gaggatgttg tcacgatttt tcataatgcc
      181 ggctatgacg gaattgccat aacaaatcat tttttcccaa gacccgaaat acccgatttt
      241 cttgatattt accgctctga ttatctcaaa gcacttgaaa caggcgacaa gctcggcatg
      301 aaggtttatc tcggcgcaga gctgagattt ccgagccaaa atgataatga ctatcttctt
      361 ttcgactttg agctttcgca gcttgaggag atgtattcct atatgacggg aacgcttgaa
      421 aattttgtcg aaaactttaa gacaaatgat atgtttcttt tacaggctca tccgttcaga
      481 gacggaatgg agctcggttt cgaaaactat cttgacggcg ttgaggtttt caacatgcat
      541 cccaatcaca actcgagggt tgccctcgca gaaaaatatg caagagaaca cggcaaaatc
      601 gccacggcag gaacggatta tcaccacatc ggccatgata atctttgcgc cacaagagca
      661 ccgtacctcc ccggaaacga gcacgaactc gtctcccgcc tccaaagcaa cgacttcgtt
      721 ttccaagtcg gcgaacattt tatagtataa tcgcttgaat taaaattgaa caaaacaaaa
      781 agctcaaccg cctcctataa cggaagcggt tttttgtgcc acagtttgcg gctcggcata
      841 ttctgttaat aggcaaaagc caaaaaacaa cccggcaaaa tatattcgtc gggtaaaagg
      901 agttaactaa attgaacaac tatccatatg aacgctccgc catggcgccc atgggatttg
      961 caaagagtct ttcatcgcca atgcaaacgg gtttcacatc ggctttaccc gaaaaccctg
     1021 ttgcaacaat ggcatatgtt ccgtttcaaa ctgacgcgac cgtttatgat gagatgaaag
     1081 ctctcagagc aggtacactt ttccccgtac tcgacaagcc cttcaagggt cgtggtatga
     1141 gatgacaaaa gcattgcttt cgcagcaaat cgatgccgta cagtttgcca tgtgggaact
     1201 gcacctctat cttgacactc acccggacga catcagcgca ttggcactat tcaaaaagta
     1261 tgaagaaaaa aacgaaaagc tcgttgccga atatgaagaa acctgcgggc cgctttactc
     1321 aaattcagac cccggcgtat catggctcaa aaatccgtgg ccgtgggact tagggggtaa
     1381 caactgatgt ttgtttatga aaagaaattg caatatcccg tcaaaattgc cacacccaac
     1441 gccaaatacg caagtattat aatttcacaa tacggcggtc cggacggcga gttgggagct
     1501 tcgctgagat atctcagcca acgttttgct atgccttatc ccgagcttaa aggattgctg
     1561 acggatattg gcacagagga gctcgggcac cttgaaatga tcggagcgat cgtccaccag
     1621 ctgacaagga acctttcgta tgacgagatc aaacgcggtg gctttgacac ctattttgtc
     1681 gatcacacaa caggtgttta tccgacagcg gcaagcggtt tgccatggtc cgccgcagaa
     1741 atgcagtcaa caggcgacac cattgccgac ctcatggaaa acatggctgc cgagcagaag
     1801 gcaaggctga cttatgacaa cattcttcgc cttgttgacg atcccgatgt tcgagatgta
     1861 atcaaagtcc tgcgcgaacg agaaatcgcg catttccagc gctnnnnnnn nnncttcggt
     1921 gaaggcttga gaatcgcaac tgataagctc aattcaaaga atttttatgc tttcaacccg
     1981 tcgtttgaca aataaagtat gatggtctaa aaaaaataag ctataattcg caattatcaa
     2041 aataattgat tgtgaattat agctttttct ttgatttatt caaccgtata ggtcaacgct
     2101 gttgcatatt tccagccgtc ggccggggtt ttgatataga ccttgaagc
//
LOCUS       DAWBSW010000265         2141 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8204_length_2141_cov_1.812081, whole genome shotgun sequence.
ACCESSION   DAWBSW010000265 DAWBSW010000000
VERSION     DAWBSW010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_2141_cov_1.812081"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1182..1191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          652 a          380 c          480 g          619 t
ORIGIN      
        1 gctttatcag tgcatttttc ataaaatcac ccatggaaat ttttgacctg aaaaattgtg
       61 tttatacata aagattattg caaaaaacga tttatcagtg tataatcttt tcattgattg
      121 aggtgacagt atgagcagtt ttgaaaaaat tatttcattg ataatccctt tttacaattc
      181 agaagaaaca ttgcaaaaat gtcttgattc cgttgccgca cagaccgttg acaaggaaaa
      241 acttgaggtg attcttgtca atgacggcag caccgacggt tcggaggaaa tagcaaagaa
      301 atttgcaagc gaaaaaacgt ttgcaacgat cattacccgt tctcacggcg gttcgggagc
      361 ggcgagaaac agcggaatta ctgcggcgag gggaaagtac atagccttcc ttgatgccga
      421 cgattcactc tctgccgacg caatgaagca tacggttgat ttttttgaag aaaacgaaga
      481 aagaacggat attgtagcct acaagatgat tccttataca aaatccggca gaaaaaagtg
      541 cgatttcaag aatgaaaaac gtgcgacgga tatatatgat ttgaacgttg aaggcaatga
      601 attcctttgt ccgaacggaa tgaatattgt tgtcaaaaac attggagacg agaatgttct
      661 ttttgacagc tcggaggaat gtgaccttaa agcgacggaa tactgtattc aatgcgcaag
      721 ggacaagatg acagtcggtt atgttgacag cgccgaatac cttaaattca acaacccgtc
      781 aggtgagagc aaggctttga agtgttccgt ttattttgac cgctttatcg gtaagtggga
      841 ggaaattttt tcaagctttg attcggttcc ggattttgtc aagtcgcttt ttgtagagga
      901 tgttctcaac agaatttcgg gggattatct tcttccgtat catattgagg gtgatgccta
      961 ccgtgatgaa attcgtagaa ttgaaaagct tctcgaaagc gtcggagatg aaataatcat
     1021 caatttcccc gatgcaccgt tgatgaacag atattatctt gtttcgatga aatacaaggg
     1081 tgaccttgat gctgttacgg acggtagctt aaagcttgtt cacgaggata aggtcttata
     1141 tgaagcagac agtgttaagc ttgtgctgac aagattcaaa gnnnnnnnnn ncggaaaaag
     1201 catataaaaa atccgagctg aaaaaatata ttttagcaaa caaaaaggtc tttgcggtcc
     1261 gcttcataaa ttatcttatg ccgaaaaagc gcatctggct ttaccatgac tgcaagggcg
     1321 tcggcgttga caatgcatat tatcagtttg tgcatgattt ttcgattgac gacggtgttg
     1381 aacgttatta tgtcgtaaac ggttcagttg acgccgtgaa agataagttt acacccgagc
     1441 agcagaagaa tttaattgcg ttccgttcaa ctaagcataa acttaggtat cttaacgccg
     1501 aaaaagtaat tacggctttc atcgaaaagg aaaattatct tccttatttc agtgatattt
     1561 attccgaata cattgatctt ttttcgggcg atgtgtatta cctccagcac ggagttttgc
     1621 acgcacatct tccgtggaag tattcctatg acaggctcga tgtgacgggc gaggtcattt
     1681 cgacaaatta cgaggtgaaa aattttaccg agaattattt cttccccgag gtaaaatgcc
     1741 gagatacgat tatttcgaca ttgacgaaac caaggcggaa aacgtcattc tttttgcgcc
     1801 gtcatggaga aagtatctta tcagtcataa gggcgagggc ggctggatgc ccgataagga
     1861 taagttcata cagtcggaat tttataagaa aacacaggca tttttaaaca gtgacaggct
     1921 tgaagaaatc cttgagaaaa acggttggac gcttgaattt aagcttcacc cgatatttgc
     1981 gccttataaa gaatgtttcc tgctgaaatc ggacagagtg aaatttgccg acggcagggc
     2041 ttccgatgaa tataaaatct gcattacgga ctattcgtca tttgtttttg attttgtata
     2101 tctcaaccgt gcgattatat atttcatgcc cgattttaag g
//
LOCUS       DAWBSW010000266         2133 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8236_length_2133_cov_2.628489, whole genome shotgun sequence.
ACCESSION   DAWBSW010000266 DAWBSW010000000
VERSION     DAWBSW010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8236_length_2133_cov_2.628489"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          499 a          609 c          448 g          577 t
ORIGIN      
        1 ctccaaaacc ttgagaatag cctcggcaca ttcattttca tcgagtatat cttccggaag
       61 tttaaatcct ttttttctga gcctgtaggc aagctctgta gcctgcggca cgtcaagtcc
      121 cagctttttc attctttcaa cgttcctgaa aacctctttc ggagtaccgt ccattgcaat
      181 tctgccaccg tccataacga ttactctgtc tgccttaacc gcctcgtcca tatagtgagt
      241 gatgagtata acggtgattc cgaactccct gttgagcttc attaccgtgt ccatgacctc
      301 tttcctgccc tgcgggtcaa gcatggccgt aggctcgtcc atgacgatac acttggtgtt
      361 cattgcaata acgccggcaa tagcgactct ctgcttctgt ccgccggaaa gacggtgagg
      421 ctcacgcttg cggaattcat acattccgac tgcctttaat gcactgtcaa cggcttttct
      481 tatctccgaa ggctcaactc cgagattttc gggaccgaac gcaacgtcgt cctcaacgat
      541 cgtcgcaacg atctgattgt ccgggttctg aaaaaccatg ccgacggttt tgcgaatatc
      601 aaccgtcttt tcctcgtctg acgtgtccat gccctcgacc gtgaccttac ccttgttcgg
      661 aacaagtatg ccgttaatca gctttgcaac cgttgatttg ccgcagccgt tatgaccgag
      721 cacagcaaga aactgcccct caggcacttc aagactgaaa tcgttaaccg cgttaacgat
      781 cccctcgtcc tccagctcgt aggcaaacga aacgttatcg aattttataa tggtatcgct
      841 catagtcagt gtttctccga attacttttt ccaaattgca gatgcgccgc atgggagaac
      901 catgcccgtc tgcgtcacag gcaagcctat ctcgctgccc gtcacctttc cgccgaattt
      961 cggcacgaca agcgcaccca aaatatactg cataacagcc ggcgaaagtc ccgttgtata
     1021 tgaatttata agcatcatta tcgggtcatc agaaagcacc tcggtgcaaa gctcaacaaa
     1081 gccgtaaacc tcatcctcaa gcttccagac ttcgccgccg gggcctctgc cgtatgacgg
     1141 aggatccatg atgataatat catatttatt gcccctgcgt atttcacgct gaacaaattt
     1201 tatacagtcg tcaactatcc atcttacggg tctgtcggct acatgggaag cggcggcatt
     1261 ttcctttgcc cagttaacca tgccctttga ggcgtcaaca tggacaacct gcgcccctgc
     1321 tttaaggcaa gctacggttg cgccgcccgt gtaggaaaac aggttgagta cctttacggg
     1381 tctgcctgca tttctgataa gctcagccgt ataatcccaa ttgactgcct gctcggggaa
     1441 aattcccgtg tgcttgaagc ccatggtttt aacgttaaga ccaagctctt tataatcaat
     1501 acgccaagcg tcgggagttt ttcttaaaat ctcccagtga cctccgcccg ttgaagagcg
     1561 gagatatctg gcattggctt ttttccacat gggatgcttc ttgggagttt tccaaatgac
     1621 ctgcggatcg ggacgaataa ggataacatc accccaacgt tcaagccttt ctccgtcgga
     1681 gcaatccagc aattcataat ccttccagcc gtcggcaact ctcattctga catcctccgt
     1741 ttatataagt ctctctttaa ctacctgtgc aatttcctcg gcaagggtct gaatcatatc
     1801 cttatccctg ccctcgagca taactctgac aagcggttcg gtgcccgaaa cacgaacaag
     1861 cactctgccc gactcaccaa gctccttttc tgcgcttgca atagctttct tgatctcctc
     1921 gtccttgtca tagcgagcct tgcccatctg ggaaactctt acgttgataa gaacctgcgg
     1981 aaaaacttcc atctcggcgg caagctcgga gagctttttg cccgttctct tcataactgc
     2041 aagcaattgg agagcagtaa gctgtccgtc acccgttgaa gcgaagtcaa agaaaataac
     2101 gtgacccgac tgttcgccgc ctatcttaaa gtt
//
LOCUS       DAWBSW010000267         2090 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8456_length_2090_cov_1.617690, whole genome shotgun sequence.
ACCESSION   DAWBSW010000267 DAWBSW010000000
VERSION     DAWBSW010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8456_length_2090_cov_1.617690"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    215..245
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1228..1237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          526 a          495 c          359 g          669 t
ORIGIN      
        1 acttaaaagt cattgccttg tcaagggcct cgctgccaag agtataggtt tctgaattcg
       61 gttgaaccgt tttactgcaa acactttgaa ccggtccggc tttatctgta gaaacagtta
      121 tcattgcctt gatttcttta atttttctgc cgggagaatt aatcgttcct cttacggaaa
      181 tgccgtcatt tttcgatgtt gtggtttgat tgaannnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnntcata tccgtcaagg gtgtcaagcc tttgcttata gctgcttgcc gatttatatc
      301 caacataaaa attaatatcg gcttttagcg gtgcctcctt tggtgtggta atccatttgt
      361 cgtacatcca gccctcttta tcgtcatact ttactcgcca ccatatgttg ccatacttat
      421 tcattatcca acccgatata gaaaccttgt cgcccttttt cactgaaatc ggtgtttctg
      481 ttttaatatg gtatggtgtc gaataaatgc cttttgaatt tgcgcccgat gcatcactta
      541 aaacagttgc ggttgcattg gtcagctggt tgttttttct catttcactt acatatgggt
      601 ctctgttatc gccaatgtca ttagtctttt tccagccatc ttttatctgc ccttttatat
      661 ggcgctcttg ctccggtgta ataaaaaaag catcacgaat acggacacga gtttttttgc
      721 ttattccttt atcagcaaaa gcgttatcgg aaaaaatctc catatggaca tgatttcctg
      781 ttgcctttcc ttctgttccc tcttgataaa aagctgttcc ttgaggaata attttaccct
      841 ctttattttt taaatagtca atgttatcat catgacctac cgatatgtac atatagtcta
      901 aacgaccatc agcataatgc actttatcct tgctcttaaa aacaattcca ttggcccctg
      961 gatcccaatc aaggtcctta atttccaatg taaaaggagc aaaaaagtag tcacgctcac
     1021 gatcttttcc ggccaagtca ttggcatatc gatatcttcc tttgctactt atttcccggt
     1081 gtttatcttc actatgacta atattcacat attccattgg aaatattgcg ttctcatatc
     1141 catctgccgc aacggatgta aacagcacat ctcccttctc cgccgctgct tccacgccaa
     1201 acagcgagct gaactcgata ccggttannn nnnnnnnaga gatgcggagc cgaagagcat
     1261 aaccgcaacg aggagcgatg caactatgcg tttaaatgtt ttcttcactt atttttcctc
     1321 ctttgtcttc ttttgctttt tcttgatgat caatgcaaga gcagcaaata cagcaatacc
     1381 tgtgataacg accgcaacca ttatttttat gaaaaggttc tccgttttcc gcaccgcaga
     1441 agcaaccttt gtgccgagcc tctgattctc cgcattcagc gattgcagag atataacggc
     1501 atcaatcgcc tcatcctctg ttaaggtatc ggagacgata tcaatatccg atgcctgctc
     1561 cctaagaccg ctctcaaccg ctgcaacaaa ctccgctttc ttttcagagg gaattgcgtc
     1621 aagcgcttcg gcgccgacac ccgacagcgc attatctatg atgctgtcaa tcactccggg
     1681 gtcaaccgca tcaactgatg tatttacaaa atccgttgcg gtcgccgcct tatatgcttc
     1741 gattatttca tcaacagagc cgaaataacc ctgcgtgcct gttattatat ttatcgtctc
     1801 gtttacgctt tcaagaaatt ccgaatcaaa atcatgatca aaattattaa ttgaatcaaa
     1861 cggcatttct ttcagcgcag aatcgacggc aacaataacg tccttataat caggctcttt
     1921 tatttcaccc gaataaatat ttgaagcctc ttcatcggtg aaagatatcg caacctcctt
     1981 gcagtcaaag atcacatctt catactgctc attgatggta tcgggctgag aataaaaaag
     2041 cgtcagcttt gtgcttttcg cttccgtttc atcaacatta aagctcagag
//
LOCUS       DAWBSW010000268         2080 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8511_length_2080_cov_2.816790, whole genome shotgun sequence.
ACCESSION   DAWBSW010000268 DAWBSW010000000
VERSION     DAWBSW010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8511_length_2080_cov_2.816790"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          496 c          481 g          584 t
ORIGIN      
        1 aagatgaaat gatcgtttac aatataatca gagaactttc cgctgaactt tttaagcggc
       61 tcgtcctttt tgccgttttt aacaagcttg aagccgtcat cggcgttcgc aaagcagtaa
      121 tacgcgccgt ccttttcacg gaactgatat gacggggagt tgacaaactc ggtcgattcc
      181 gcttttctga cgcttcttga gctttcaacc gacatgaagt catagagaac cgtctctgcg
      241 ttggtgtcgt gatatctcgt cgaagcgaac caatcgtatt tgctcatgct gtttacggag
      301 ctgtcggtga acaccgtcca cgtttcgttc tgagttgcgt cctcctgaac cgttctgtct
      361 cttgaggaaa cgatcagagt tgccgttcct gaattcatat caaatgaata tatgtcggaa
      421 tatgttttgt caaccttgta agcgtcctcg gcttccatgt caagaagcag aatataatct
      481 gtccttgccg actttttgta agaggacggg cagtccataa gacgggcaaa aacataggaa
      541 aaggtctgaa catcgctttc ctgcttggaa ttaaaaagtc cgtagccgat agtgccgaca
      601 tctgtatcct caagataata gagtgtcatc gggattttct gatagctgca ggttatgctt
      661 atttttttcg ttcttacatt tgtaatctca acaaattcct tgtcggaata tttgaaatat
      721 ttgatttttc cgtcggggta agtttcgtag aaaacatttt cctgctgagt ttgaacaagc
      781 ggatagtcgg acttgtattc catgcttaca acagatgaaa gcggttctcc gccgtcgatt
      841 ttcattccgc cgaaaagctt gcctgtgaag cttttgctga atgtaagccc cgtaaaaaca
      901 aggcagacgg caagcacggc cgaaagaatt atgataacct ttttgttttt ctttgtcatt
      961 gtaaatccct cttattttaa aacaagttcg accggacaat gatccgagcc gtaaatttcc
     1021 gaatgtattt taacgctttc aagcctcggt tcaagagcct ttgaaacgca gaaatagtcg
     1081 atacgccagc cgacgttctt ttcccttgcc ttgaatcggt atgaccacca actgtaggcg
     1141 ttctcaaggt ccgggtaaaa ataacggaag gtgtcaataa atcctgcgtc gagcagctcg
     1201 ccgaattttc ctcgttcttc gtcggagaag cctgcgttgc ctctgttcgt tttcgggttt
     1261 ttcaggtcaa tttcattgtg ggcaacgttg agatcaccgc agaaaacaac cggctttttc
     1321 ttctgaagct ccgagacgta agctctgaaa gcatcctccc atttcatacg gtggtcgatt
     1381 cttttcagcc cgtcctgagc gtttggaacg tactccgtga tgaaatatat atcgtcaaat
     1441 tcgagcgtaa taagtctgcc ctcggtgtca agctcctcaa ttccgagacc gtagcttacg
     1501 ctgagcggct cgtccttggt gaatattgca gtacccgaat agcccttctt cagggcatag
     1561 ctccaatatt gacggtagcc gtcaagctcc aaaacgagct gaccttcctg cattttagtc
     1621 tcctgaaggc agaaaatatc tgcgttcagc tcattaaaaa cgtccataaa gcccttgttc
     1681 atacaggctc ttatgccgtt gacgttccat gaaataaatt tcttgctcaa atgaacaccg
     1741 cccaatatag atgttaatta cagtgaaaaa attttgcctc ccatgcaggg tagcaaagcg
     1801 gcacggcggt agttgaactt ccggcggacg ttcggagccg gagcgtgacc gagccgcagt
     1861 gagacgctgt cagcgaaagt gatagtagac tcctcctccg gggtagcgaa gtgtcgacac
     1921 ggtagtgaat gacagtccgg tggactgtca gagccgtggc gtgaccgagc cgcagcgaga
     1981 cgctgtcagc gaagctgact gagggagtgg aatttcatca aacttaaact ccctccgtca
     2041 cgacttcgtc gtgccacctc cctcaagagg gcggccacga
//
LOCUS       DAWBSW010000269         2073 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8546_length_2073_cov_2.122398, whole genome shotgun sequence.
ACCESSION   DAWBSW010000269 DAWBSW010000000
VERSION     DAWBSW010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_2073_cov_2.122398"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1191..1200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          615 a          462 c          445 g          541 t
ORIGIN      
        1 gatttatgat tagagtacaa atcgtaggtg tcgatgacca catcgaccca agttcagaaa
       61 aagtttaatc taggtgcccc tgtatggaag ccggctgagt ggtttttata aaatctttta
      121 caagattaaa aaagaaaaag gacgtgaccg aagccacgcc ttttcctgcc acacaaaaaa
      181 ttgtatacat aaccgttttg aattagcatt atttaattat cttacttctt ttaagcttgg
      241 gagtgaaaag cataaggaat gggaatatct ccagcctgcc aagaagcata tcgagaataa
      301 atacgacctt tgaataccaa ctgaaatcgg aaaaattgcc cgtcggtccg acttctccga
      361 gtccgggacc gttgttgagc gttgcgataa ctccggaaat atttgtaacc atgtctttac
      421 cgtcggttga gacaatcagc agggaaacaa caaaaactac aacataaatg aagaaaaata
      481 catttgttcc cgttactacc tcatcactga tacgcttgcc gtccattgtg ataacgctca
      541 cgcttcttgg gtgagcaagg cgctttagct ctcgccttgc cgatttggca agaatgacaa
      601 ttctcgaaac cttgaatccg ccgccggtac tgcctgcgca tgcacccacg cacataacga
      661 tgagcattac cgtcctgctg aaaacaggcc atttatcaaa atcggttgtt gcataacccg
      721 tagtagtcat taccgaactg acctggaatg ccgactgatg aagcgcctca aagaacgagc
      781 cgaatatatg ccggatattc aatgcaatca gaattattga cgttgccatt attcctatgt
      841 aggacttgac ctcaacgttt ctgaacgctg ctttcagttt tccgcagaac agaagaaaat
      901 agaagttaaa attgataccg aaaagcatca tgaaaacgcc gattattatc tgtgtgtata
      961 ggctgtaatc agcaatgctt gaattgacga tagcaaatcc gccggtacct gccgtgccga
     1021 agctcagcgt tgtgctttca aagaacgaaa gtccgccgca gcgaagcaga agaatctgca
     1081 cgatcgtcat tataacgtag attccgtaaa gaattctcgc cgtgcttttt gatttcggca
     1141 caagtttttc aacgtccgga ccggggcttt ccgccttcat aagctgaaga nnnnnnnnnn
     1201 gctgaagatt accgccgcct tttgcaagcg gaagaatcgc catgatgaac gcaagtgcgc
     1261 ccataccgcc tatccagtga gtgaagctgc gccagaaaag cattcccttg ctcagtgctt
     1321 caacgtccgt cagtatgctt gagcctgttg tagtgaagcc tgaaacgacc tcaaaaacgg
     1381 catcgaggta attcggaatc tccttgctga tgaaaaaggg cagagctcca aaaagactga
     1441 taaggatcca gccgagcgaa acgattgcaa agccgtccat tgaaacgatc gttttgtcat
     1501 gcggtttaaa tctggtcata acaaagcccg aagcaacggt cgctgctatc gttatgagaa
     1561 aaggaacaac attttcctta taataaagag caatcaatgt cggcaaaagg aggagtgctc
     1621 cctccgtgat aagaagaata cctaaaacat aggttatcat ttttttgttc atggcgtcag
     1681 tccttaatga tatcgtcaag atcggaaagg ccttggatcg tagttacgac aacaacggaa
     1741 tcgccgacct gaagcatatc cttacctcgg gggatgaaaa cattgccatt acggttaatt
     1801 gaaccgataa taacattttt gtgaagaagc agctcctcaa gagaagtgtt aacaatgtca
     1861 ctttcctcgc tgacatgaaa ttccaatgcc tcaaccttat tgtcgattat tttgtagagc
     1921 gtctcaacat tactgccgat gttgttcttc tttgcacgga cgtgctgaag aatctgactg
     1981 gctgttatga atttgggata tataacactg ccgatgtcaa gtgagcgcac aacctcattg
     2041 aaagacgtac tgttgatttt tgcaattgtt ttt
//
LOCUS       DAWBSW010000270         2014 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_8916_length_2014_cov_2.127616, whole genome shotgun sequence.
ACCESSION   DAWBSW010000270 DAWBSW010000000
VERSION     DAWBSW010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8916_length_2014_cov_2.127616"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1119..1128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          487 a          497 c          498 g          522 t
ORIGIN      
        1 cgccggaaac gatagaagcg attctttcgg gaatgttttt gccgcatgcg atataatcaa
       61 ggaagaaaag cggctttgct ccgcagcaaa taatatcatt tacgcacatt gcaacgcagt
      121 caatgccgac cgtgtcatgc ttgtccatga gaaaagccag cttcagcttt gtgccgacgc
      181 cgtcagtgcc gctgacaagc accggtttct cgattcccgt tgtgtcaagc tcaaacaggc
      241 cgccgaagcc gccgacatcg gagcacacgc cggatgtcat tgttcttgca atgtgtgatt
      301 tcatcagttc gacagccctg tagcctgccg ttatatctac gcctgctgcg gcgtagctct
      361 cggaacgtga ttgtgtgttc atcacttttt ctccttttgt ctttcggaaa ttttacattc
      421 aaacttgctc ttgtgcgtct cggtcggcac ttcggtcggg taatcgccgt cgaagcaggc
      481 tgtgcaatag cccttgccga ggtcgccgca ggcaattttt ttaacattgt caacgctcag
      541 ataaccgagg gaatcaacgc cgataatctc tgcaatttcg tcaaccgtat ggtggcaggc
      601 aatgaggtta tcacgggagt cgatgtccgt gccgtaatag caggggttaa ggaacggcgg
      661 agcggaaaca cgcatatgaa tttcctttgc gccggcattt ctaagcagct taactattct
      721 tgcgcaggtc gttcctctga caatcgaatc gtcgatcatg acaactcttt tacccttgat
      781 aacgtttgtg atagggttga gcttgatgcg aacctgattt gtacggctct tttgacccgg
      841 agaaatgaat gttctgccga tatacttgtt cttgataaat cctatttcat agggaatacc
      901 ggactgcttt gcgaagccga gagcggcatc aagtccggaa tcgggaacgc cgataacaac
      961 atccgcctga acagggtgct caagagccaa aaatgcgcct gcacggattc tcgcttcatg
     1021 cacgcttgct ccgtcgatca ccgaatcggg gcgtgcaaaa taaatatatt caaagacgca
     1081 ggtgtgcggc ttctctttgt tgcaatggtc cttaatgcnn nnnnnnnnat tccgcaccga
     1141 ctgcgtcaag cgcacagctt tccgaagcga caacgtagcc gccgttctgg ggaagcctgc
     1201 cgatacaaag cggacggaag cccatttcat cacgaacggc aatcatcttg gtaggcgaca
     1261 ttacaacaag ggaataagct cccttgatac ggttaacggc acggttgaca gcctcctcaa
     1321 tcgagggagc ggtgagtctt tcctcggtta tgatgtatga aataacctcg gtgtccgatg
     1381 tcgtgtggaa aatcgagcct ttaagctcaa gtgcactgcg aagctcatag gaattggtga
     1441 gattgccgtt atgagcgagg gcaatatgac cgttaatgtg attgacgact atcggctgag
     1501 cattgtccct tgccgagccg cccgttgtgc cgtaacggac atgacctatc gcgatattgc
     1561 cctgaccgaa ctggttgaga tggtcggtgg tgaaaacatc gttgacaaga cctgtatcct
     1621 tatatgcggt aaaaagtccg tcgtcgttta caacgattcc gcaggattcc tgtcctctgt
     1681 gctgcagggc gtaaagaccg taataaacgg tacttgcgat atcataagat tcattggcgt
     1741 aaacgccgaa aactccgcat tcttcatgta tattagacat aaaaagctcc gtttcttatt
     1801 tgaaaactct cttcatcatt tctgcatagg catcttcaac tccgccaagg tcacggcgga
     1861 aacgatcctt gtcaagcttc tcgtttgttt tgctgtccca gaagcggcag gtgtcgggag
     1921 aaatctcgtc ggcgagaact attgtgccgt ccgagagctt gccgaactca agcttaaagt
     1981 caacaagagt tacgttaaga ctcttgaaat actc
//
LOCUS       DAWBSW010000271         1968 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_9201_length_1968_cov_2.484056, whole genome shotgun sequence.
ACCESSION   DAWBSW010000271 DAWBSW010000000
VERSION     DAWBSW010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9201_length_1968_cov_2.484056"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1712..1721
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          523 a          458 c          456 g          521 t
ORIGIN      
        1 ctcacacccc gatatgctca agggtattgt tgcgattcgt cagcttcata tgagctcgct
       61 cagggattac ggactttcct ctgccgacga gatgctctat cccgacaatc acaggtatct
      121 ttcggatttg ctttcgtacg ttgccgtcgg cgcaagatcg gttgaaaatc agcagcacag
      181 acttacggcg agcggacttg atgttcccgt cggacttaaa aacccgacag gcggcgacct
      241 ctccattatg atgaatgcca ttactgcggg tcatcacagc catacattta tctatcgcgg
      301 ctgggaggtt gagagcctcg gcaatccgct ttcccatgca attctcaggg gcttccagac
      361 taccgacgga cagaatgtgt caaactatca ctatgaggat ctcgttaaac tcaatgagct
      421 ttatcagaag tcgggacttg aaaatcctgc cgtaattgtt gacacgaacc attcaaattc
      481 aggcaaaaag ttcagagaac agccgagaat agctcttgac gttatccaca gcatgaggga
      541 aaatcccgat atcaaaaagc tctgcaaggg tctaatgata gaaagctaca ttgaggacgg
      601 aagccaaagc acagacgatg cggtttacgg caagtccatc accgacccgt gcctcggctg
      661 ggaaaagaca gaaaagctca tctttgagat agcagataag ctgtaataaa caaacaggct
      721 gtgacgaacc aaaattcatc acagcctgtt ttattattat attataatgt aatcaatatc
      781 cgctgttgtc gtagaggtag cccttttgtt caaagtaatc aattctctca ttgccgtctt
      841 taacggtgaa aacataggtg cagtcgcctg catagtacat attttcttcg ccgaaaagct
      901 gagtaaaata gcgtgtgttg tcgcactcgc ttgtcatggc aatatagcgg cactgcggaa
      961 tgagaagctt ctccgcttta acattgttat aaatatttct gtcgtcattt atgagatgat
     1021 gagcgcactg aagatatttg caggaaagcg agtttctgcc gtaaagcgca agcagagctt
     1081 ctccgtttgt ttcctccgca tcgccgagaa aaatgacgga gcaatcgtca aaaatgatct
     1141 ggaaaaccgt agatgtttcg ttaacgcttc tgtagacgtc gttgccgctt gcacgggtgg
     1201 agcgggggag tatgtcctca tgagttgaga gaacctcgat tctcgcatct gggaaacgca
     1261 ccgtttgacc cgtgtggagc ttgagaaatt tagcgttcgg agcgtatttt ttgattctgc
     1321 ttctaagctt gtttgcacac gggattccgt attcaagcaa ggtctttgat gggaagttga
     1381 acatcacacg ctcaacgtcg agaacatcct tttcacgctt gagaagcttg atgaaaaaat
     1441 ccatatggtc gtcgtgattg tgggtgcaaa ggtaagccga aacacggatt ttttcgcctc
     1501 tttttttacc tgtaagatcc tcaaggcggc gcataaattc atcacatgct tcctccgtgc
     1561 actgctcaat ctcgccgccg tcaatgatga taatggaatt gtcacgcatt ctcattgcgt
     1621 aaagcatgcc gcagtcgcag ctgtggaagc gaatcatctt gccgtaacgc agagagaact
     1681 gcataagcgc cgtatcgcct cgaacgtcgt cnnnnnnnnn ngcattgttt cggcagtgta
     1741 gtatgtataa ataattctgt caccgtcgga aaactcggca aaaatattgc cgttgaattc
     1801 acggtgaaat gtctctttac gtcctgttcc cacaagtgaa gcaagaaaat cacggtacat
     1861 tttagacgtt acatcgccga gaacgagcat atatgagtcg tcctcaccgc cgtttgtttc
     1921 gtcatcaatc gcaagacctg cgccgcagtt tattggttct cccaagac
//
LOCUS       DAWBSW010000272         1935 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_9413_length_1935_cov_2.660106, whole genome shotgun sequence.
ACCESSION   DAWBSW010000272 DAWBSW010000000
VERSION     DAWBSW010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9413_length_1935_cov_2.660106"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1706..1734
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          599 a          396 c          329 g          582 t
ORIGIN      
        1 acatatgagc acggaagtcc gccgatattt ttgatgaact gtccgatgag aactaccacc
       61 atattggtcg gacaaatcca gcatattgca ctgcctattg aataaagagc aaatcctgcc
      121 aatgtaaggt ttctctttcc gaatttcttg gcaagcggcc atacggcaaa tatgccgata
      181 cccatcggaa taccgccgat gacagaaatg agcgtttgag ttttgccgtc gttatatgtg
      241 ccgagaacat agttacagaa ataaaccaag ccgagatttt taaactgcgt accgatagta
      301 taaatcagga aataaagcag aataagtatc atatatttgt cggtaaaaat tgcttttaaa
      361 agatttttat acggcaggct ttcctcttga ggtttgtcaa tatcttcaag agttaccctt
      421 tcttttgtaa aataatactc cataagggta agcggcaatg caattacaga aagagcaccc
      481 attacaacta tccattttgt cttgtccacg ccgagcatcg gcataacaac catcggaaat
      541 acaagagcaa cgataattcc gctcatcatt attgttgaaa tctgattgaa aaccgaaagt
      601 gagcctctct gctccgtatt tcttgttgaa agaggaacca taagattgtg actcatgttg
      661 tatattgtat atgagaacga atagaaaagg ttatatgaaa gcataaccca aatgacctgc
      721 acggtttgac tgctctgcgg cacggtaaaa agaagaatac cgctgattaa aattaaaggc
      781 gcggaaagca gcagccacgg ccttgctttt ccctgtgctg tccttgtgtg atcaattata
      841 tagcccatta tcacatttgt aattgcatca attatctttg aaacaaccgg gaatacagtc
      901 agaaaggctc cgccccaaac cgttgtcagc tttaaaacat cggtataata gacattaaga
      961 taggtagcga gcactgcatt gagcaacagc gcacccgacg gtcctaaaag atatccgagc
     1021 cacttttctt tcgcttttac ttcatcgctt ttaatccgac tcgcaaaaag cggagagttt
     1081 aacttctttg tcataattta tctgctcctt aatcaaataa tttcgccgtt ggacttgatc
     1141 acttctttat agaaatattc cgaatcctta attgtgcgct tttgtgtttt ataatctata
     1201 tgtataattc cgaatctcgg cttgtatccc agtgcccact caaaattatc cataagcgac
     1261 cagtactgat agcccataac ctctgtaccc tgattattca tacacacctc ataataatta
     1321 tctattattt acattttatt ataacaacac attgtcgaga ttacaatcat cagtttatgt
     1381 attagtggct tttgagacaa catatttgac atattttgtt gctttaataa taaatcggca
     1441 taaaaaaacg aaaaaattgc taacagaaaa aagaaattaa agcttgtatt catttttcgc
     1501 cacattgaga ttgatacgat aatttttaca tcaaattcaa aaattgccgc tactctttca
     1561 agtaacggct atgtaatata tttaggcaat aaaaaaagac cgataatctc tcgaaaatca
     1621 atcttttata cttgcttcat acacgtcggt ttaatagaat tttagtaatc gtttactgtg
     1681 ccgctattat ctttatacct tattcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacaaag
     1741 cactgcctcc gccgagtgtt taatactgca agcggttgcc aacatagtaa tatttgtgct
     1801 ccaaaccaac attgggaata gttttcgatg ttctcaatcc gtcaacgtca taagtaaagc
     1861 caatatttat cgttctgaac ttcatcgacg caagctgtct gccgttctgc catgtgagcg
     1921 tgtaaccccg gtatg
//
LOCUS       DAWBSW010000273         1923 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_9488_length_1923_cov_2.144004, whole genome shotgun sequence.
ACCESSION   DAWBSW010000273 DAWBSW010000000
VERSION     DAWBSW010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9488_length_1923_cov_2.144004"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1075..1084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          579 a          386 c          390 g          558 t
ORIGIN      
        1 gtttctaatc ctttcagcgt caaccaaatt gtgtatttca gaaattttgg agcgatactt
       61 tggcataaat tcttttaatc tatctgccat atttttactg acagcaaaaa tgtggctgct
      121 ggcatcatat ccgaaattat catggtaaac tcttcggaga tcgctgtaat gataaaatga
      181 aatgtaatta tctgccgtta ccgatctgag agtcaagccg gcaacttttg agctgtaaat
      241 taccgctgtg tcaaaatggg tgtcggataa attgcgggct atatatcgtc catacagcca
      301 ttcttctaaa tttctaaaga aaaatcttgc aattctgttt ttaatagttt taatcccggt
      361 tgaatagttg caagcgtcaa taaagccaaa aagtttggca aacttatacg gagaagcgaa
      421 tgatacagta tgatatcgat cggcaaatat cagcttacac actttcggta tattttttga
      481 tatttgctga ttgtcggaag tgatcaagca ggtaacatca tacctgtcat agtctattct
      541 gttaagaatt gaaaccagtg cagtctcaat tccaccgatc cacagagcgt tgtgtatgaa
      601 tattattttc tttttcatat catttctcaa actcgctttt ctccggcaat atcgtttcaa
      661 acgcgttttt gacttttgcg gaaagagctt caaaatctat atccacatcg gggtcattga
      721 ggcagatcat atcatggctt gcgtttgtta ttatgtcgca aagcccgtcc acagtgttgt
      781 catcaacacc ggagacaaca ttatcggtca taaacggtga gaatttgcct gaagcgatct
      841 gccaccagag catgacccac tgattgacat cgtcggcgca gcggaaacgg tgggatgatg
      901 tgcgctcaag cagctcgggc acggaagccc acacctcgtc gaatgtgctg cggagatacg
      961 cagcaggagc gtgcagattc ataaaaccgg tgaagtaatc agggaaaagc cgctctatca
     1021 cctttgtgcg aatattgtcc tgccagcgat aggccgaatt tgcatatttc ctgcnnnnnn
     1081 nnnnaagtat aaacatatca tcattgaaat atacaaaatg ctcgctgagg cccggtattc
     1141 ggtgaagatt tgcctcgata acatgtgaac tgaaggttgg caaatattcc accggaatat
     1201 agtccttatg gttgactatc tttaatttct cgtgttcggt attgagaaat gctggtatat
     1261 gcccccatgt gacaaaataa atcttattta cccacggagt atatttctca acagctctga
     1321 accaataagg gagcagaccc caatcacggt aacggttggc ggagttgctc tcatccgctt
     1381 ttgtcggaga atacttgtcc ttttcggttt tccatttcgg gtcgttgccg tcgacccaag
     1441 ggataacaaa gtcaattttc ataattcaaa tcctttatgt gacgagagaa cctattcggg
     1501 gaatcctccg aatagcccat gaaactataa gcgaaagaag aattgttaca acagtgaata
     1561 acggcacccc aataatcgga gggataaatg tggggaagaa atcaataaaa gtaaataagc
     1621 tgagtataag cggatgagat aaatagattc caaaactgta ttttgcaata tatgatattt
     1681 gtttattgat tctcttaaaa ctacgtctcc cgaattgttg aaacaggaga taaattgaaa
     1741 cagtttcggc agcaacattc caagttaaat aatcagctga tgtctcatcg gcaacaccgg
     1801 tcgaccatga aagcttaatt acagatacaa tcgaaaaaac tgtcccaatg attccgaata
     1861 catagcagat cctacgtata tttgcagata gtttgtacct ttttaaatac cagccgagaa
     1921 tat
//
LOCUS       DAWBSW010000274         1918 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_9523_length_1918_cov_2.940955, whole genome shotgun sequence.
ACCESSION   DAWBSW010000274 DAWBSW010000000
VERSION     DAWBSW010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9523_length_1918_cov_2.940955"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          523 a          444 c          385 g          566 t
ORIGIN      
        1 cggaataatt tgaaagaaca agctttcctt ttgaaagatc ataaccctta ggagctttaa
       61 atttaacaga accttcccta aatgagcaaa caacgagaac tctttcgttt ttgagaactc
      121 tttcataggt ataaaggaaa ggattgagat gatcgtactc cttatatttt ccgtatatga
      181 ggacttcatt ttcttttctg agcctgataa gctttttgta aaaattaaga atcgagtcgg
      241 gatttttaag gtcgtcctca acattgattt ctttataatt cggatttata ttgaaccaag
      301 gcttgccggt tgtgaagcct gcattctccg cctcggacca ctgaacgggc gttctggaat
      361 tttcacggct tacgtccttt gcgagcttca aaaatctctt ttcgctgtag ccgagcttct
      421 tgaaaatgcg ccagttgttg aaggtcataa cgtccttgca gtccgaaagc ttatcaagaa
      481 ggatattcgt catgccgatt tcctgaccct gataaataaa cggtgttccg caaagaagga
      541 agtacatcgc cgcaagcatt ttaccgcttt caacacggta atttatattg ccgtaacgct
      601 cgataattct cgcatggtcg tgattttcga tataaaggca gttccagccc ttgccataca
      661 aaccgtattg ccattttgaa agcgttgatt taagctttat gagattgaac ggcgttttga
      721 cccagtcaac catgaaacag ttggcgttca tgtgctcgaa cgatatcatc atattaagct
      781 tctgattcgg accctcggta acaaaatcct tcgccatctt aatatccgac attgggcctt
      841 cgccgactgt aaagcaatca taatgatcat aaacgtcacg cttaaattca tcaagatatt
      901 cctcaagatg cggcccgtgc ttataatgct caacacccgt tgcaacggga agcggaatac
      961 cgtcgggcaa gccctcacgc ttggaaataa acgttataac gtcctcacgg aagccgtcaa
     1021 cgcccatatc aagccaatat cgcatgatat ccttgacttc ctgtctgacc ttcgggttat
     1081 ccatgttaag gtcgggctgt ttttttgcga aaacatgaag ataatattca tcaatatcgg
     1141 gattatactc ccatgctccg ccttcaaagg tggaaagcca gttgttaggc ggcttattgc
     1201 ccttaccctt gcgccatatg tagtaatcgt gataagggtt atcaacgctc tttgcgctct
     1261 ccttgaacca ttcgtgctca tcggaggtat gattgacaac gagatccata aaaactctca
     1321 ttccccgctc atgcgctcct tcaagaattt tcttgaaatc gtcaagggtg ccaaactcgg
     1381 gattgatatt tctgtaatcg gaaatatcgt agccgtagtc tgcgttaggc gtgggatata
     1441 tcggtgaaaa ccaaattccg tcaacgccga gctccttgag atagtcgagc tttgaataaa
     1501 cgccaggaag gtcacctatg ccgtctccgt tgctgtcaca gaagcttctc gtccagattt
     1561 gataaacgca catttctttg taccatgttt ttttgatatc agccatgtaa tcaccccgat
     1621 ttttgtttta ttataacata accgcgtcat aatggcaaca aaaaaatggc tgaacatgtc
     1681 agccatttgt gtattgatat tgcaaatcgt catacatcgc cgattttggc tttgcatttg
     1741 atttttccgg atgataaaac caaagcgtaa accaactgtg aagctttaag ccctcaaccc
     1801 agtgtctcgg aaatccgccg ctctcggcgc aaagaaaggt ttcgtctgac atacgcagaa
     1861 aggcatgctt aactctgccg ccgcaaagct cactgagagt atcaacacag atttgttt
//
LOCUS       DAWBSW010000275         1872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_9829_length_1872_cov_2.224546, whole genome shotgun sequence.
ACCESSION   DAWBSW010000275 DAWBSW010000000
VERSION     DAWBSW010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9829_length_1872_cov_2.224546"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          478 c          441 g          536 t
ORIGIN      
        1 gtcgattgcc ttatcaggca ggaatctgtc gccgatgtat cttgacgaca tcttaactgc
       61 ggcttcgata gcttcatcgg taatctttac cttatgatga gcctcatatt tgtccctgat
      121 acccttgagt atctcgacag cctcttcacg gctcggctcg ccgacaagaa ccgactgaaa
      181 acgacgctca agagcggaat ccttttcgat gttctttctg tattcttccg ataacctgca
      241 tttcacccct tgcaagagcg ggctttaaaa tatttgcagc atcgacagca ccctctgcgg
      301 cacctgcgcc gataagagta tgaacctcat caatgaagag gataatgttg ccggcctttg
      361 aaacctcgtc gatagccgac ttgattcttt cctcaaagtc gccacggtat tttgtacctg
      421 cgaccatgcc ggtcagatca agagcaacaa ttctcttgtc cttaagaatt tcgggaacct
      481 cgcccgatgc aattttaaga gccaagccct cggcgatagc ggttttacct acgccgggtt
      541 caccgataag gcagggattg ttctttgtcc tacggcaaag gatctgaata actctttcga
      601 tttccttctg tctgccgata accggatcaa tcttgccctg ccgcgcaatg gcggtaagat
      661 ctctgccgaa ttggtcgagg gtcggtgtct cggtttttcc tttggactgt tccttggatt
      721 tcggacttgc gtcgttctgc gaaacattga cggattttgt aatttcctcg gcaatgtctg
      781 cagccgaaac attcattgcg tccaaaatat ccattgcgta gcaactgctg tcacggagaa
      841 ttgctataag aaggtgctct gttccgacat agccgtggcc gagacttctt gcaatcgtga
      901 ccgccgtttc aatgatattt ttgcttctcg gtgtgaaatc gttcggcgaa agaactgtcg
      961 gagtgccgat gccgacgctg ttcctgacag cggtctcgac attatccgcc gtgatgtttc
     1021 ttgccgtgag ggcagtgtat gcgacgcttc cctcgcttga aagcagaccg agcagaaggt
     1081 gctcgctgcc gatatatgtg tgaccgagat tctccgcata ttctattgcg gcattaagag
     1141 ccgtgtttgc tttctgagta aagcctgtaa acttatacat aaaaatctct cctttgttta
     1201 ttgaatttgc ctccattgca gggagcttat tgcttttaaa aacgggacct gtctgcgatt
     1261 gcttttgcaa tccgacaagc cccgtattta atcaattatc attggtcatt caaaaacacg
     1321 aggcttttaa aattcctttt gcatctctta ttcaaatgcc tttcttaccc attcggctcg
     1381 gatgatatct ctctcctgcg gagaaagctc cttgccctca agagcgttga ttgtggcagg
     1441 ctgaaccttg accgaaagct cattgagctt tgtaatatca aagtcaagcg tcttgtttga
     1501 aatgcccatt ctgagaagcg atgcaagctc catgaactca tcgccgctga gaactcttgc
     1561 gttctgaaga ataccctttg cacggaagat tttatcctcc tcggcaggat tttccagaag
     1621 cttttcagcc gcagcacgtt cctgcgcaac gagctgtaat gtaattgact ggagattgtc
     1681 cagcgcagtt tcctcggaaa ttccgagcgt tatctggttg ctgagctggt agatatctcc
     1741 acgggggctg ctgccctcac cgtaagcgcc gcggattaca agtccgagct ttgatacggt
     1801 tgcggcgagc ttgccaatct gaccgcagcg ggtcaaggcg ggcaggtgaa gcatgacgga
     1861 ggctctcatc gc
//
LOCUS       DAWBSW010000276         1854 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_9969_length_1854_cov_2.171206, whole genome shotgun sequence.
ACCESSION   DAWBSW010000276 DAWBSW010000000
VERSION     DAWBSW010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9969_length_1854_cov_2.171206"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          533 a          400 c          426 g          495 t
ORIGIN      
        1 ttaaaagaat aagcaatcat gcatttaatt actgcacaaa gcttaaagaa attgagatcc
       61 cgaaatctgt tgaattcatt gattcaacgg ctttcagctc ttgccagagc ttgcaaaaag
      121 cagtcatctc cgcacaagtc aaatatatag tgggcgatgc ttttgccaag tgcgacaagc
      181 tggtgatcta ttgctacaaa gacacggcgg catattatta tgcgagcgaa aacgatattc
      241 cctatgtcat tcttgagtat cccgatacac aggagcttga tgcgcttctt gacgcataca
      301 aaaaacttaa ccgagataaa tattttgagg agtctcttgc ttcgctcgat gaggctgttt
      361 caaatattga tatgacaata acggtcatca ctcaggaaat ggttgataaa tgggtatcgg
      421 atatcaagaa cgcaatgaag cagcttgaat atctcccggc tgactattcc gagattgaag
      481 ccgccaaggc gagagccggg cgggttgacc gctcgcttta tacgcaggaa agccttgcga
      541 ttcttgatga agcgctttct tccgttgacg aaactctcga tgtttcggat caggacttgg
      601 ttgccgcata tgcaaaggca atcaacgatg caataaacaa tcttgaatat cttccggctg
      661 attacaagaa agtaaatgag gcgattgccg caagtgaggc gatcgatcgg ctgctttatt
      721 cgcaggcaac ccttgcgatt cttgaccaga gcatagcggc cgttgactac tctcttaaaa
      781 taattcagca aagcatagtt gacggctttg ccgacaggat caattccgca atttcggccc
      841 ttaaatatgc tgatgttgtg cttcgcaatg agccaaacgg cgttatcgtc agcgcaacgg
      901 caaaggagat ttatcccacc acatcgctta cggttgatat gcttgacccg tcaaattttg
      961 aaaccgcaaa cttcgccgtc ggcggataca ttaaaaatgt gaaatattat gatatcaatc
     1021 ttatccgtga cggcgcaaag attcagccgg atgggactgt atccgttaaa atcaaaattc
     1081 ccgatgatgt gaagcccgaa aaatgcaggg tttatcacgt caccgaggat ccggttgacc
     1141 cgcttgttcg ctttgcgagc acgctggacg gcaactatat tgagacatat ttgagcggta
     1201 tttcaataac caaaatgccg tcaaagcttg tatatgcgct caatgagaag cttgatttat
     1261 ccgatatgga agtaactgca ttgatgagcg acggggattc gcagataata gccgattacg
     1321 atgtttcaaa tgttgatact tcctccatcg gcagcaaaac ggttacggta tattacactt
     1381 ataatgatgt tacaaagagc gtttcttttg agataaccgt ttcggcggac aagctcgcgg
     1441 caaatattac tcttgacggc aaggatttaa gtgaatacag caaaaaagtc aagtggtata
     1501 ggagctattc gagcgaatcg ctccggcttg agtgcaattt gcccgatgcg gataattaca
     1561 caattaattg gagctccgat aactccaagg tgatcgttga tcagagcggc aaagtcacaa
     1621 gcaaaggctt tttcttccca agggaagcga ctgtgaccct taccgttacg gattcctcgg
     1681 gcaatgtgct cgcaacggac agcatccgca tccgattcta taaattcgca ttcccgtttt
     1741 cggggattca gaccgttttt gggctgctaa aaagaagcgg aatggttttc ttttaagatc
     1801 gaatattaaa ttgaatatta acttgcaagc tccgctgtgg tctttcacag cgga
//
LOCUS       DAWBSW010000277         1847 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10020_length_1847_cov_1.883929, whole genome shotgun sequence.
ACCESSION   DAWBSW010000277 DAWBSW010000000
VERSION     DAWBSW010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10020_length_1847_cov_1.883929"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    820..875
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          571 a          379 c          412 g          419 t
ORIGIN      
        1 gtgcgttaat gcgtgtataa ttgattgtgt ataaagaatt atacaatttt aatcattctt
       61 gaaggagttt atatgacgga aaaaaagaca agctttgttt taccgacgga tcttctgagc
      121 gagctggagg atttttccga tgatgaggtc ggaaaaattt tcagagccgt ccttaattat
      181 acaagcgaat ccgcagagcc gtgcttcggg gacagggcaa tgaaggtcgt tttcaggcat
      241 atcagaaaat acatagacag cgcaaacgaa aactatgata aaaaagtgca ggcgaacagg
      301 ataaacggtg caaagggcgg caggccgaaa aaggaagccg ctgaaaaccc aacggttttt
      361 gaagaaaccc aacggttttc ggaaaaaccc gacaaaacct atactgagtc tgagactgat
      421 tctaagtctg aaattgagtc tgagtctgag agtgagcttg agtctgtcag caaagcgctc
      481 acgcgcacaa aaaaatacgg cgaacataat aatgttgaac tgagtgacga tgaatacggg
      541 cgtttggctg agcgaatggg tgtaagcaac cgcaattcgt atatcgagaa gctcagtgac
      601 tatatggctg cgacgggcaa aagatatcag agccattatg cgacgattcg caattggttc
      661 aggcaggacg gcggcacaaa gccaagctac gacatggacg aaattatgag gcgagccgaa
      721 caattacctg tttacaagag caataaggaa aagaaaaaag agaatactta atatcattat
      781 cggctcaaaa aaagaacgaa agccgttatg gccctcgttn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgga ttgtatttat tgcagctttt
      901 tccactcggc gacagccagt cggtcgcagc gttcgttttc cgggtgggag gcgtgaccct
      961 tgacccatac tacggtgacc ttgtggatat cgcagagcct taaaagctcc tcccacaggt
     1021 caggatttaa cgcaggcttg ttgtcgcttt tcttccagcc ctttgccttc cagctctttg
     1081 cccagccttt tgaaaggccg tcggcaacgt attttgaatc ggtcgtcagc gtgacctcgc
     1141 aaggctccct gagaagcttc agtgcctcga ttacgcccgt cagctccatt cggttgtttg
     1201 tcgtgttgcg ttcaccgccg ctgatttctt tctccgtacc cttatatctg agaacagcgc
     1261 cccagccgcc gggacccgga ttgcccgagc atgcgccgtc ggtaaaaatt tcaacctttt
     1321 tcattgcttc acctcgcctg aaatatttta ccataaggca cggaaaaaaa caataattat
     1381 ttcttgcaac gaaattgttt atgaaataga gctttaaccc ataataatta gcataaatca
     1441 ttgacttttg acgcaaaaaa tagtatcata aattagttag attgggtaat tatgtttatt
     1501 ctatgtctat aatgcggttt acataaaaat tttaaagaat aggagttagt ttnnnnnnnn
     1561 nnaaaaagct gacaagagtc agaagtctgc agcaaaaaac aagcagacaa aacgcggcgt
     1621 aaagcagccc caaaagactg ccaaaccgcc gaaaagcaaa acggcgaaga gcgaaaaatc
     1681 gaaaactgcc aaatctaatc cgaaaaacgc tcaacaggcc gaaaagagaa ggacaaatca
     1741 aaaaagaacg aataagaaaa gcttcggttc aacaaattca agaaggaagc agagcaagaa
     1801 aatgcccgcg aaatccgtta agataggctt cctcggcgga cttaacg
//
LOCUS       DAWBSW010000278         1842 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10061_length_1842_cov_1.949636, whole genome shotgun sequence.
ACCESSION   DAWBSW010000278 DAWBSW010000000
VERSION     DAWBSW010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10061_length_1842_cov_1.949636"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1210..1219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          496 a          420 c          363 g          553 t
ORIGIN      
        1 ccgtttacct ctgccacgat tttcggagta tacttggctc cccactgaga gaaaagatga
       61 gaaatttcaa agtatttaat tccgcatttt tgagcgagcc tgacccatcg gataaacctg
      121 tcaaaggcaa agctgtattt accgttttca agcttaacgt caacgagctg aaccgtcagc
      181 ctttcaccgc cgacctccgt atcgagcgga ggagtgaaaa ctggggtgag cagcatatta
      241 acacccgtat gaaccgccgc tttaatgaag ctttcaacga gcgcccaaaa tttctcgcag
      301 aaaacgggaa tcttataata attcgctatt gagtcgctgt gaaaccattg agtgtaaatt
      361 aacttttgct ccggcagaac agcatttata acatgcagaa cgaatatgtt ttcactgatt
      421 gtctcgccgt ctttttttag ctttattttg atttcataat cgccggcgtc cgtatccttc
      481 ggaagatcaa ccgtaatcca aagcgcataa aagttttgac gtttaatgag tacacggtta
      541 ttttcaatcg gaaaaagaac atcggggaaa agcccgggtt cattcctttc acaaaattca
      601 caatcggatt cgtaaacggg aagctccgag ggaacattgc cgaccgacct gaccgttata
      661 aattgagaaa gaggggagtc aattacaatt tcggcgaaaa atttctcact gcttttatat
      721 gcgatttgat atgaataacg ctctcctttg agcacagaat cgcaattatg ctccgttaaa
      781 tcacatcttc cgtcgagaaa aacccgctga agtgatgaca cctgtccggc tttaaaatcc
      841 atgctttcaa ctcccgtttt tctttaatca tatcagattc ggtcttgttt gtaaatattc
      901 atctgcgttt atttgcgctg actattcctc cgacagccga gaataccgtc attattattg
      961 ccgatatcct gcccgagacg gctattcccg tgcggcttat gattgacgca acaagaaaaa
     1021 taatcaaaaa tgcgggcaag gcgctcagcg caccgaatag caacccgttt ttgcgaattg
     1081 caccgactga cttaaatccg ccgaagaagc cggatactgc ggagattgca aaaataaagt
     1141 atttgtacat cgacaaatca aaatcggctt tgagactaac caacgaaaat atcacgcaca
     1201 atgccgaatn nnnnnnnnnc gtttaatgcc gagccgacaa gaatttttaa aattatgttt
     1261 cttaaattcg ttgattccgc ctttggcttt ctgcgtttgt ttttgggcat aaaaatccct
     1321 cctcacaaaa tgatatgaga agggaatttt tattattact gatttcgttt gatctttgat
     1381 gtggttgttt ttttaaattc tttatccact atttcaaatc gtttaattga cttatagctt
     1441 acgacctgtg aatttacact cttaacggct tcggaaacag ccttgctgat ctctttatct
     1501 gttatgtttc tgttgccgta ggttgcgccg atagccttaa gatcggggaa aattcttgcg
     1561 taaacaattg tatttcctcg ctcgtcctca gcgcccgtta cgatacattc cttgacggcg
     1621 ttttctttaa gaagcatact ttcaagctcc tcgggataaa cgtttttgcc gttagtcgtg
     1681 acgataacgc tcttgcatct tccgcagatg tagtaatgac cgtctttatc cgttcttgct
     1741 aaatcgcctg tatgatacca gccgtcggaa tcaaaggctt ctcttgttgc ctccgggtcc
     1801 ttataatagc ccagcatgat gctgtcgccg cgcatgcaca gc
//
LOCUS       DAWBSW010000279         1839 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10070_length_1839_cov_2.820628, whole genome shotgun sequence.
ACCESSION   DAWBSW010000279 DAWBSW010000000
VERSION     DAWBSW010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10070_length_1839_cov_2.820628"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          531 a          361 c          404 g          543 t
ORIGIN      
        1 cctttttgct gttcatcatc gacgaaccga tgattgtcat tgcaccgccg acaagcatac
       61 cgacgcctat tccttttgcg attgaagaac ctgaattttt cataatctga cctccgtttt
      121 attattgaat aaccgctgaa taaatcagtg cttcgacatt attttgtctc cttatgaatt
      181 gattattccg aagtattatg ttataatctt ggaaagaaat tctttcggag ctgattcttt
      241 tgaaaatgaa aaagcaaaat agagctgtgc tcataaatga tatgtcctgt ctcggcagat
      301 gctcgctcac tgcggcaatg ccgattattt ccgcttgcgg aatagaaagt gttccgctgc
      361 cgacgggaat tttttccgca catacggaat ttgaaggatt tgttaaagaa gatttgaccg
      421 atgacatcgg caagatctca aatcattgga aaagcttggg tgaaagattt gactgcatct
      481 acagcggcta tcttgcaagc tgcaagcagg cggaaagcgt aaaaagattt ttgcttgatt
      541 ttaaaaaaag cgacaccctg tatattgccg accctgttat gggtgacaac ggtgtttttt
      601 ataaggggat tgatgactcg tttatttccg aaatgcgttt tctttgctcg ttggcagata
      661 ttattgttcc caatgtaaca gaagcgtgca tgcttacagg tatggagata actgagaatt
      721 acgatatgag ttttatcaaa gagctgctta tctcccttcg aaatcttact gccgcaagaa
      781 tagttgtaac gggggttgac ttcgatgacg gacaaatcgg atgtgccgtt tatgacagtc
      841 ttatgggaag agcaaatatg ttctttactc cgaaatccga aggacgtttc ccgggcacag
      901 gtgatgtttt cgcttcggca ttgacggcgg cagctatgaa cggaaaagac tttttcgacg
      961 ttgttcaaat tgcaatgggc ttcacctgca aatgcgttga agcaacgctt gaggcaggca
     1021 cagacaggaa atacggtctt tgctttgaat ccgagattaa aaatctcatt aaatctgttg
     1081 aataatttac atgtaagaag gatttgaaaa atggcagttt taaatattgt tctcgttgag
     1141 ccggaaatcc cgcagaatac gggcaacatc gcacgaacct gtgctgcaac gggaacgaga
     1201 cttcacataa tcgagccgat gggattcact ccagacgaca aaaaactgaa aagagcaggg
     1261 cttgattact ggcacctgct cgatataact tattataaaa atctggacga tttctttgat
     1321 aaaaacccga acggagaata tagatttttt acgacgaagg cgcagaaccg ctatgttgac
     1381 gttgaataca gcgacaatgt ttatcttttt ttcggcaaag agacaaaagg cttgcctgag
     1441 gagcttcttt tcaaacaccc gaatgaatgt gtgcgaatcc cgatgataaa cgactctgcg
     1501 gcaagaagcc tgaacctttc aaattcggtt gcaattgccg tctatgaggt tctcagacaa
     1561 tggaattatc ccaatctgct cagtgcggga aagctgacaa aatttgactg gaaagacgct
     1621 gaataaaaaa agaccgacac acttcaataa atgtgccggt catgtttttg aaaattattt
     1681 tgttgtgctt ccgaagccgc cgttacggat tcctgttgca ttatcgtcaa ccgttattcc
     1741 gtattcaacg aaaatgcctt gtgcaaaacc tgtacctttt ttgacttcaa cggttttatt
     1801 ctcgttggag tcatttgtta ttttgataaa gatatgacc
//
LOCUS       DAWBSW010000280         1828 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10159_length_1828_cov_2.246475, whole genome shotgun sequence.
ACCESSION   DAWBSW010000280 DAWBSW010000000
VERSION     DAWBSW010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_1828_cov_2.246475"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1491..1500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          481 a          371 c          416 g          550 t
ORIGIN      
        1 ctccctgctt gaaatgctcg ggataagcta cacagagccg agttttaatt ttatcgttcc
       61 gctcggaatt tcgttctata catttcagag catgggctat gtgattgacg tttacagggg
      121 taaggtcaat gccgaaaaaa atttcggaaa gcttgctctt ttcgtctcgt tttttccgca
      181 aattgttcaa ggtcctatcg gaatttattc cgaccttgct catcagctct atgagccgaa
      241 aaggttcagc tatgaccgct tcaaaagcgg cggacttctg attctttggg gcatttttaa
      301 aaagcttctg atagccgaca gagcagttaa ggtcatcaaa ctcgtgactg ccgacagcgg
      361 tgcgttttcc ggtacattca ttctttttac agtcatattg tacgccgctc agctctatgc
      421 cgatttctcg ggcggaattg atatctgccg cggcattgcc gagataatgg gcataaaaat
      481 ggcggagaat ttcaaacgtc cgtatttctc gaaaactctt accgagtatt ggcacaggtg
      541 gcatgtcagc ctcggcgatt ggcttaggaa ttatcttttc tatccgatct ctatttcaaa
      601 gccgttcctc aaaatgtcaa agagcatgaa aaaacacggc atgaaatatt tcggcaaggt
      661 tttcccgacc gcacttgctt cacttataac ttttatagtt atcggcatct ggcacggggc
      721 agaagcaaag tatatcgcct tcggattttg gaacggactt gtgattatga tctcgagcct
      781 tatgaagccg ctgacggata agctcaccga aaagcttcat attaaacgtg aaagcttttt
      841 ctattcggtt ttctgtatgc tcagaacctt catggttgtg ctcatcggat attatttcga
      901 tatcgcaaag ggctttaagg atgcaatgag catgatttgg cgttccgtta ccgctttccg
      961 tataggtgat ttcgcttcct ttgcctgcct tgccaaaagc aaaatagata tgttcgatta
     1021 tctgataatt ttcggcgggt gtacggtgat ctttataatc tcgataatac aggaaaagaa
     1081 aaaagactcc gttcgcaatt tgctttgcga aaagagtctg gcattgcagt ggcttttata
     1141 tattttaatg atagttgcga ttgctttgtt cggttattac gggccgggaa cgactccggc
     1201 agactttgta tatatgcagt tttgaggaat tgaaaaatga aaaaatttct aagatttatt
     1261 tgctttctcc ttgttgtttc aacggtgatg ttcggcttga attttgctct gagcaccaac
     1321 agcgataagg acggcataca cataagagga tatttcaagg agccgaagaa tactgttgat
     1381 gttcttctta tcggtgcaag cgagctttac acgggcttta attctccgct tgcatgggag
     1441 aaattcggct ttacaagcta tggcttgagc tttgccgcaa tgccgggggc nnnnnnnnnn
     1501 ggggcattat ataagactgt tacacaaaag gcgctgaaaa ctcagtcgcc gaagcttgtt
     1561 gtgttcgaga taaacggctt ttttcacaaa gaaaagaata gcttgcagcg cagaccgatt
     1621 catgcttggt ttgacacagt cggcattgac tccgacggaa agaaatttct tgaacaaaac
     1681 attcccgaaa gggaacacat tgagtattat ctgccttttt ataaatacca tgcaaattgg
     1741 cggaaaccgc ttatctgtgc acgaaatgcg atgagcagac ttacgcttga aatcgagggg
     1801 ataagctatt ccaaggcatt tgcgaata
//
LOCUS       DAWBSW010000281         1825 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10192_length_1825_cov_2.414689, whole genome shotgun sequence.
ACCESSION   DAWBSW010000281 DAWBSW010000000
VERSION     DAWBSW010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10192_length_1825_cov_2.414689"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1112..1121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          488 a          420 c          361 g          546 t
ORIGIN      
        1 aaaaccattt acgaccacat tcaaaaataa tctcggaccg tttcattttt gtaaaaaatt
       61 tgaagctgtc attttgtttc tttatgttgc atttttcgac gtttgtgata taatatcaaa
      121 tcggtgataa ttatgaaata caacacatat atttttgatc ttgacggaac gcttcttgac
      181 acattgcagg acttggcaaa tgccgtgaac tatgcgatgc gtcaaatgaa ttatcccgaa
      241 agaacaattg atgaggttcg tcgcttcatc ggcaacggta taagaatgtt gattaagcgt
      301 gccgctccgc aggacatttc ctcagaggat tacgacaaaa cgcttgcgat tttcacggca
      361 tactaccttg accatatcgc agacttcaca aagccgtatg acggcattgt cgacgttata
      421 aagacgctca aacagaacgg ctgcaaggtt gccgttgtgt cgaacaaagc cgacgaggcg
      481 gccaaaaagg tagttgaaga tttcttcggc gacatcttcg acctcgtcgt cggcaagatg
      541 gatcgctttc cgtcaaagcc tgcgcccgat tcggtgcttt acgtcataga aacgctcggc
      601 gcagacaagt caaaatgcgt ttactcgggt gattccgagg ttgacgttca gaccgcgcac
      661 aatgcaggcc tcccctgtgt cggcgtaaca tggggcaacc gtgacgtttc cgagctgatc
      721 acggcgggag cggaatatat tgccgaaaag ccgtatgaaa ttatcgaaaa gacaaaatag
      781 aaagaagctg atgcagattc gaccccgcat cagcttccat tatttattca gaaagtaaat
      841 attccgtagt atgacgtttc gcccttttcg gttatcttgt caaccttacc gtttttctgc
      901 ttcatagtga actcggagct gtacggcgtt ttgacattca gctccttcac cctgccgttt
      961 gtgtccgtta cagcctcaat aaccgtaccg ctgtaggtta cgctgcccga ggtatagtcc
     1021 tttatgaacg ttccgtcata accgaattca aacgggaagc cgccggcggc attgtaaacc
     1081 gaatcctttt ttacgtcaac cttttcggat tnnnnnnnnn ngccgacgaa ccgatggtca
     1141 gcgccgctgc aaatgtaagt gccgtgagaa ttgaaataaa atttttcatt ctatatcatc
     1201 tcctcgtcgt tataatatca aaatgatgtt gtctgtaacc gatttcagca atttcacttt
     1261 tgtagatgtc accctcataa tcgaacagaa tgctataata gctctcatac ggaacataga
     1321 catcaaacga ttcaacatat ccgtcttcgt taataaatgc cgtgactttt gttcctatgt
     1381 acataatttg accaccgtta tattcaaagt cgtccgtttc gtcattgata taatagaacg
     1441 gtactcctcc gcaatactga atttcgggga cttcagaaag cccggcttct tcaaaccaca
     1501 attccaggtt tacttcatat ccgtttttta tcttttttat ggaaatttgc ttaatcattt
     1561 cttccgtcag caacgagtta ggcgcaatat cttcaggagc gttgccgtat tcatcaagtc
     1621 cgtccgtgaa gaaataattg tatgtatcag gtccgaaatt cattctctgc ccttcatcag
     1681 tcacgggaat atcgacccat aatccataat catctgtcac agtttcctct atttcctttg
     1741 tttttgcata tgttttatca agtgcactgt tataaaaaga caatatctcc tgtttttcgg
     1801 acggcagctc atatgttttt tccaa
//
LOCUS       DAWBSW010000282         1797 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10424_length_1797_cov_2.343858, whole genome shotgun sequence.
ACCESSION   DAWBSW010000282 DAWBSW010000000
VERSION     DAWBSW010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10424_length_1797_cov_2.343858"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          582 a          329 c          414 g          462 t
ORIGIN      
        1 tttcttgacc gtgatgagaa cggtaacgaa atgaacaagt attttgtcga taaccctgaa
       61 atggttctcg gcgaaatggt tatggaaagc tctcagttcg gtcagtcatt aacctgtaaa
      121 ccttatgagg acagaagtct ttcagatcta ttatccgaag caatacagaa tattcatgcg
      181 gatttttccg aacttgatgt ttccgaaata gacgagattg aggacaattc aattcccgct
      241 gaccctgatg ttaaaaattt cagctttacg gttgcagacg gtaaaatata ttaccgtcaa
      301 aacagcagaa tgaatcccgt tgatgtttcg gcaaccgctg agaacagaat taagggaatg
      361 attaaaatcc gtgattgtgt aagagagctt atacggttac agacagatga tttttccgac
      421 agcagcatta agtcggaaca ggataaactt aattatatat atgactcgtt tacaaagaaa
      481 tacggactcc tcaattcaag ggcaaacagg ctggtatttt ccgatgacag ttcatattgc
      541 cttttgtgtt cgcttgaaat aatcggtgaa aacggtgaat tgaagcgtaa ggcggatatg
      601 ttcaccaaaa ggacaattaa gccccacgtc aaagtaaccg ccgtttcgac ggcaagtgag
      661 gcgttggcgg tgtctatcgg agaaaaggca agagtagata tgccgtttat nnnnnnnnnn
      721 tcacgaggaa aagtatcttg cctccgatga atatttatcg ggtaatgtaa ggcaaaaact
      781 tgaattggca aaaagaacgg cgaaacttta tcccgaggac tataacataa atgttgagat
      841 gcttgaaaag gtacagccgc aggatttgtc tgcgagtgaa atatcggttc gtttgggagc
      901 tacatggctt ccgaccgagg atgttcagaa ctttatattt gagctgctgc aaacaccccg
      961 ttatgctcaa tggaatatca aggttcatta ttcggaatat actgccgagt ggaacatcga
     1021 aaacaagaat tatgataaat ccaatgttat ggcgaacagc acatacggaa cggaaagagt
     1081 aaatgcttac aagattatcg aggaaactct taacctaaaa gatgttcgta tttttgatta
     1141 cgttgaagat ctaaacggca acaagaagcc tgttcttaac aaaaaagaaa ctgcaatcgc
     1201 tcagggtaag caggagatga taaagcaggc ctttcaggat tggatatgga aagatcccga
     1261 acgcagggaa cgccttgtca gaaagtataa cgatttgttc aattcgatga gggcgagaga
     1321 gtatgacggc agtcatatta ctttcagcgg aatgaacccc gaaatatctt tgcggccgca
     1381 tcagataaat gccgttgcac gaattatgta tggcggaaac tctcttcttg cacacgttgt
     1441 tggcgcaggc aagaccttcg aaattgttgc ggcggcgcag gagagtaaaa ggctcggtct
     1501 ttgcagtaaa tctctgattg ttgttccgaa tcatcttacg gagcaatggg cttccgagta
     1561 tttacagctt tatccgtcag ctaatattct tgttgccacg aagaaagatt ttgaaaccaa
     1621 gaatcgaaaa aagttctgtg caagaattgc aacaggggac tatgacgcag taattatcgg
     1681 tcattcacag tttgaaaaaa tacctgtcag catcgaacga cagcaataca tgttacaaaa
     1741 acagattgat gaaataacag acggaataac ccaggtcaaa gctaatcgag gagaaag
//
LOCUS       DAWBSW010000283         1794 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10444_length_1794_cov_2.004025, whole genome shotgun sequence.
ACCESSION   DAWBSW010000283 DAWBSW010000000
VERSION     DAWBSW010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10444_length_1794_cov_2.004025"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          470 a          446 c          367 g          511 t
ORIGIN      
        1 aaccctgaca gggtcctcaa ggaatctttc cttgacggca agagcctggt catagtcggc
       61 gacaaagagc ttcacgctca ttatttcatc gtccgcatca accacggaaa tggtcacgtt
      121 gcagcccttc tccaccctgt ctatccggat tttattttca cgctcgcgtc ttagtctcgt
      181 ctgaagtttg acggcggcgt tgaaagcctg ttctctcacc gtaaagggaa tatcatgctc
      241 caaagtctcg gagtttttct tgcccttatc ggtaataata agttcgtttt cttttataat
      301 aacgttaccg ttatcggcaa gctcactgag agcctgactc atttcaaaat aattagcaag
      361 ctcctcaccg catattatct catcaagctg ttctcttgtg agcggtgtat cgagacggct
      421 gagaagatac ccgataagaa tttttatctc gtttttcgtc ctcagtccgc caagctcaat
      481 gccttcgctg aaggaatcgt atcccatagg agtacctcct tattctatcg gaatttcgac
      541 aggctgtctt tgtctgaaaa taagcacgct gtcaaaaccg cagttttttg caagttgcat
      601 gccctgttcg attcccgcgc ccatatgctc aacaaaatga gaatcagagc cgaccgtcac
      661 atgctttccg ccgagctcct tgaagcggcg cacatagctt tcgtcgggca gggttctgcc
      721 gataggctgt ctgagacccg aagtattgat ttcaagcggc ttgtccttct caataagcag
      781 cgagaaaatc tcgtcgatgt ccttttgata tctcgccatg tcaacgtcga tatgatgttc
      841 accgacaatg tagcgcaacg gataagtaag gtgagcatat gtgtcaaact tgccccactc
      901 ggcaagcatc ttcatctcaa gaaaataatc gtcaagaagc ggataaacat tatccttgtt
      961 ataatccagc tcgcagaaat cgggagtttt tcttaggtta tggatcgaac cgattacaat
     1021 atcgtaatcc ttggaggcga tgagagcctc gctctctgca aggtcatatg tcggctcgcc
     1081 catctcaacg ccaacgcaaa cctcaattct gtccttgtac tgctccttag ccctcaaaat
     1141 atcggcaaag gattcgtcgg cggtcttgtc aaaatgattc tgacggaaca gatccatctc
     1201 aacgtgatcc gtgaacgcaa cgatttttat gcccttttca atcgagcttt caatcatctc
     1261 gtcaacggaa tagtgaccgt caaaggaatg aatcgtgtgc gcatgcatat caacaatatt
     1321 attaaaactc acctgtaacc ataacctttc aaatgaatac aataaaaaac tttatctaat
     1381 aaattttatc acaccgagct ataagctgtc aaccgtaatt gcccattctt tcacaagttc
     1441 gtcaacgctt ttagcggcat tggcagggct ggtggacggc aggcgcatgg cttctattcc
     1501 cgttgcgggc aaaatatact ttttatacag cttgtcggcg gttgcaccgt ttgtaaaaat
     1561 acgtttgatc ttactgtttt ttataataaa accgatatcg ttcggcacag cgtttttaat
     1621 tgagctgtcc gacgatccgt atatatcgca agaagcgata acatcccaaa gcgcaatatg
     1681 actgttcaaa agcacggctt tcttttcgtc aaccgtgaca ggctcttcgg ctttaagaag
     1741 tgtggaaacg accttccaaa acctgttcct cgggtggccg taaaaaaaca tctg
//
LOCUS       DAWBSW010000284         1766 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10647_length_1766_cov_3.165985, whole genome shotgun sequence.
ACCESSION   DAWBSW010000284 DAWBSW010000000
VERSION     DAWBSW010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10647_length_1766_cov_3.165985"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          435 c          397 g          501 t
ORIGIN      
        1 ctcgaacccc tgacccactg cttagaaggc agttgctcta tccacctgag ctacaagcgc
       61 attagttctc cacattgcag aatgttcgga gaaaatggag cgggtgatgg gaatcgaacc
      121 cacacgacca gcttggaagg ctggaattct accactaaac tacacccgca tatctgcaac
      181 gcttgatatg ataccattaa atctcggttt tgtcaagtaa tattgacgtt tttttaaaaa
      241 aatactgatt aaatttttct tatgtccgga acggcggttt tgagccgctg ctccggatac
      301 ttataacaga tttctgaaat aatcaacgat tgcttttgct gtattatcgt tgacatttac
      361 cgtgtgcttc gcttctttca tgttttcaag gtagtgcgca tcggagctga ttataacgga
      421 catatctttt ataatcggat atttctccat gtattcgttc cgatcggctt gttccgtcat
      481 ttcaactgcc ttgaagttca tctcgggcgt tatcattccg aggttggaaa tcacgctgta
      541 ggagcttctg tcaacgtggg cggggtagca tactccgccg tattcactga catatttaac
      601 cgcattttca atgctgattc cgcttgccgt tgtcaacagc agctcctgtg ttccgattat
      661 cccgtcgccg ctgtccatga ttaactggtc gccgaaaata tcgggtcggt ttttaaccgg
      721 cggaacggtt gaaagtacat attccgaaaa agcttccgca ttattcactt catcaaacaa
      781 gcacacaacg tgcacttcct ccgaggtaca aagctccatg ccgggtatga ctgtcaggcc
      841 gattttttca ccgacctcaa gagcggcacg gcaattctga gcggtgttgt gatcggtcag
      901 ggcaatggcg tccatgccga gaatctttgc catattaaca aggttatacg gcgtcatatc
      961 cttatcgccg catggagaaa gacacgaatg aagatgaaaa tcatagtata attccatttt
     1021 aaatcagcct tgaaatctcg gcggcaacct gataggagtt cttcgccgtt gtgagaatta
     1081 ttacgccgtt ttgctcggct cttttgagag cgtcctcatc aagcgcggca ttttcggtca
     1141 aaattataca ggcaacgtcg gtgagaacgg caacgccgat agaattaacg ttgcccataa
     1201 ccgtaagcca tgcgtcgccc tcctgtgctc tcgacatgac ccaactgagc aggtctccgc
     1261 aatagcagcc tgttacgggt ctatcaagat catcgccgca gatcagagtt aaatcaagct
     1321 tttctttaag tgtttttatg tccatttcag cttctcctcg tcaataatct cgcggaacgg
     1381 aacgggaata aatttttcaa tctcattctc gttgagcgga gtggtgtgac tcatttccgc
     1441 cttaagacgg aaaacgcagt ggtcaatgct tgcatagcct cggacaatat cctcggcgag
     1501 cgaacggcag gtcggtgcgc cgcaggtgcc gcagtcgagt cccgggaaat tcttcaccat
     1561 ttccgagacc tggttcataa gcttcattgc atcggtcaca ttgtcggcaa gcttcagtac
     1621 cgagtacggc tgtaaattct cttgccacat catctcgttc ggtacttcgt ttgcatctat
     1681 atgattgcat gaaaccggac ggtattgctt cagaattgac agcctcgttt ttgcaacaaa
     1741 ggtgttcgca accgtaaggc aaccgc
//
LOCUS       DAWBSW010000285         1736 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10881_length_1736_cov_2.316478, whole genome shotgun sequence.
ACCESSION   DAWBSW010000285 DAWBSW010000000
VERSION     DAWBSW010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10881_length_1736_cov_2.316478"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          470 a          403 c          398 g          465 t
ORIGIN      
        1 ccgccaatga taaaaacttt ttcatgtata tcctcctgtt tgtccctgtc gggaatttta
       61 gaatctgcct gcaccgaaag ctcagtgcgg acaacttatg ttatgacagc tccgcgaaac
      121 gcaggctgct cctatatgct gattatataa tatttttttg atgagtgcaa gaaaaacgct
      181 ccgcaatccg aagataacga agcgttacta ttctgtttta acctaaacca aaagtccgcc
      241 gttgataaag agggttgcga agccgaagta aatgagcagc gtaagcgcat caacaatagt
      301 tgtaatcatc ggtcctgcca taagtgcagg gtcgagatgc aaaagctttg ccagaatcgg
      361 caatgcacag cctatcacct tggcgacgat aacggcgcaa atcattgcaa ggcaaacaac
      421 tataaatgtg ttgcttgaaa tgtcattgtg cgttaccagc ttgatgatcc acattctcgc
      481 atagttaaca acggcaagcg tcaagccgca gagaacggca actcggattt ccttccagat
      541 aatcttgaag aaatccttga tgttaatatc gccgagggca agtccacgga taacaagggt
      601 cgaaacctgg ttgccgcagt ttccgccggt gtccataagc atgggaatgg aggcggtcag
      661 accggcaatc attgaaagct tatcctcgaa gccctcgatt atctgacccg taaaggttgc
      721 cgaaaccatg agaataagga gccacacgat tcggtttttg tagagggtaa aaatgcctgt
      781 tttaagatat gaatcctcag agggcaacag caaagccatc ttttcaaagt cctctgtgtt
      841 ttcgtcttcg atgacgtcaa cgatatcgtc aatggtgatg ataccgacaa gtctttcttc
      901 cttatcgaca acgggaattg aaagcaggtc gtatttcttc gcaattgcgg caacctcttc
      961 ctggtcgtcc atcgtgttga cgaaaatgag ctgctcatcg tcctccatga tgtccttaac
     1021 gatctgattt tcgggagcaa caatgagctt gcgaagcgga accgttccga caagcttgcg
     1081 tttatcgtcc atacagtagc aggtgtaaat cgtctccttg tcgagagcgg tttttcttat
     1141 catttctatc gcctgtttaa cggtataggt ctcagggaaa gaaaccatct cgatagtcat
     1201 gatgcttcct gccgaatttt cgggatattg aaggaatttg ttaataagct ttctcgtgtc
     1261 cggctttgtg tgggcaagca cacgggttac gacgtttgcc ggaacctcct caaggaagtc
     1321 aaccgcatcg tcaaggaaca tgtcctccat aagaaattca agctcggaat ttgtgattgc
     1381 gtcaacgata ttaccctgaa tatcgccggg caaataagaa aaagcatcag ccgatgatgt
     1441 cttgggcaac aagcggaaaa ggcgaacctg gttttccggc tccatctcct caatcaaggc
     1501 ggcaatatca acgacattca tttcgctgag catttccttg atggcagaaa atttgttgtt
     1561 ggcaagcatc ttgctgatga tttcaaccat agtgtccaat tttaagcgcc cctttcagca
     1621 cggcacaaaa ccgcgctgca ggcgtaattt cagctccgat ctcgcgagag gacgtctgca
     1681 acggtattca tgctgtgcaa attattgaat ttgttcaagt ttcgttgccc gtagct
//
LOCUS       DAWBSW010000286         1725 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_10980_length_1725_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAWBSW010000286 DAWBSW010000000
VERSION     DAWBSW010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10980_length_1725_cov_2.500000"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          512 a          386 c          332 g          495 t
ORIGIN      
        1 gttatttgtc gttgtggagt cagtgaccca cgcattaaag cttgcgtcat tggcggtcga
       61 gccgacaaat gtcataacca tatccattat aattacaatc caaacgcttg ttgtaaggat
      121 ttttgccgca tccgtaatac ctaaaacaga agcgacattg tctctcgaaa taagtccgaa
      181 gccgccgata ataacgcccc aaactatgta gcccacagaa ataaatttgt ttcttgaacc
      241 tttacggtca cttaatgcgc ccatgatgag cgttgtaagc atggcggtta ttgccgaata
      301 agcaaccatt ttactcacag catcaataac attgattgaa ccgtcaatcg ccgcctggct
      361 tgcaccttta taaatcgaat tatacaggaa ggtgttgaaa tacatgtttt caacattcca
      421 cgcaatctgt ccgatgagtc cgaacagaat aagattaaaa ataagcctgc cggagagctt
      481 tccggctttt gccttatcgc tcatataaat ccccctaaaa aatttacctt tgtagttata
      541 ataacataat acaaaggtaa aatcaagaaa aactgtgcaa ttttaatata ttttgttcta
      601 tttgactaac atcaaaagcg ttccgccgac ggtgagaaaa agaccgaagg ctgtcttttt
      661 tgataatttc tcatgaaaaa ctatgtatga aaaaagtatc gtgacaagta tgctgagctt
      721 atcaatcgga acaacaacgc ttgcgagtcc gtcatgaagc gccttataat agcaaagcca
      781 gcttccgccc gtcgcaagtc ctgaaaggca gataaagcaa agttcttttt ttgcaatgcc
      841 cttaatctct ttttgcttgc ccgtgacaaa aaccattacc catgacataa caagcactat
      901 gcctgttctg attgccgtac cgagatttga ttcaacatcg gaaatgccga ttttaccgag
      961 tatagcagta aggcttgcaa acaaagccga gccgagcgca tagaaaatcc agcgtttatt
     1021 cttcggcaca tcgtttttga catccttttt ctctatcata agaaacgttc cgacagcgat
     1081 gagcgcaatt ccgattccgc tgtaaagagt tattttctct ccgagaagaa ttgccgcaag
     1141 cagaattgtt aaaacggtgc ttgacttgtc aatcggaacg accttattta tatctccgag
     1201 ctgtagggct ttgaaatagc aaagccagct tgcaccggtc gcaagtcccg atagaataag
     1261 aaaaatcaat gagtgcggcg ctattgagcc gatctctctt tccgagccga caataagcac
     1321 catcagccat gagaaaagaa gcacaactat tgttctgatt gccgttgcaa cggttgaatc
     1381 tgtcttttta ataccgcatt ttgcaagaat tgacgtcagt ccggcaaaga gagctgagca
     1441 aaacgcaaac acgatccaca taaccgtcat ctccgattta taaactgttt ctatcttatc
     1501 actgttattg aaaattttca atgataattt cactttatat ttataaactt attaagtata
     1561 taaagcaaag cacaaccccc gaaccttttt gagattcggg ggtgttgtta tgctgtttgg
     1621 gttgtctgtc tgtcggttta aatcagtgtc ccgtcagagt ggctgtgccc tctctgttcc
     1681 agcctgcttc aaatttgccg gctacggtgt agttctgtcc cgaat
//
LOCUS       DAWBSW010000287         1706 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_11150_length_1706_cov_2.777710, whole genome shotgun sequence.
ACCESSION   DAWBSW010000287 DAWBSW010000000
VERSION     DAWBSW010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11150_length_1706_cov_2.777710"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          424 c          331 g          501 t
ORIGIN      
        1 cctctatggt tctcaaggtg tatattctcg aatcgtttat tccggggata tccggaatta
       61 tcgggacggc tcccggagcc agtatcagct tatcatagct ctcccggtac tcctcgtccg
      121 tctcaagctt ttttattgca acttcttttt tctctctgtc tatcgaaacg gcttcatgca
      181 gagtacgcac ttctattctg tagcgtgacc ataatgtttc gggggtctgc aaggtcagct
      241 cgtgctcgtc ctttatcaat cctcctatat agtacggaag tccgcagttt gcatacgaga
      301 caaatccgga tctttccaga atgataatct ccgctttctc gtcaagcctt ctgattcttg
      361 ccgccgcagt cgctccgccg gcaactcctc cgataattac tactttcata tgtgtgtcct
      421 cctttacgct ttcggtaaaa aatattaagc aaaaacgcac aagtcaatcg cgaaaggact
      481 caacttgtgc gttattttgg caaaaagccg catgaccaat gatacccgat ctcacttcgg
      541 tatcggcagc tctttgaaaa cattcgattt atgcgttgtt ttaacataac tccacctctg
      601 ctgaaacaat tgcatatatt gtagacttaa tataccattt ttaatatcat aatgtcaata
      661 cttttgaaaa aaatgttgat ttttttgcga caaaaaaacg atctgcatat aaaaatgcag
      721 accgttaaat atacatttgc cgaaaaaacg tcagatcttc ttgcagaaag tgaaaatctc
      781 ctcgggaagc tcttcctcat cggcggagat tgtgaagatg aagctcttct cctcaacgtg
      841 attcttgctg agctcgtcaa ccttcttgag gaactcgcaa agctcgccgt aatcacgtgt
      901 accgattctc agcgttgcgt caacaagaac atccgtaata tcgtggtcac ctgcacagat
      961 gccgcaaagg aaaccgaaga atgcgtcata accgcttacc ttgtaatgat ccgtgtcgat
     1021 aagacgtgcg cgataattta catcatatgt gagcttgggc tgcttttcaa cgcaaacaac
     1081 gttgccgttt gaattctcaa tagcctcgtt gaccttttca atcaaaagct ttgtttttcc
     1141 tgaacctttt ttacctaaaa taagagatac cataataata tcctccctgt ttttttattc
     1201 taacattcgt gttcctgcac gaaaattata cgaaatttaa tgcggcatcg ccttagccta
     1261 atcttgcctc aagctcggcc ttgcgatcct cgtatcccgg cttgccgaga agagcaaaca
     1321 tattcttctt gtagctctca acacccggct ggtcgaacgg attgatgccg agaatatagc
     1381 cgctgatagc gcatgccttt tcaaggaaat agataagata gccgagattg aattcgtcca
     1441 ttgcttcaac ctcgagcaca atgttcggaa cgtcgccgtc cgtgtgagcg agcaatgtac
     1501 cctcaaaagc cttttgatta acgaacgaaa cagtctttcc tgcgaggaaa ttgagtccgt
     1561 cggcactgcc ctcctcgttc tttatctcaa cctccgactt tgactttgca aagtggacaa
     1621 cggtttcaaa aagatgtctt tcaccctcct ggatatactg acccatcgag tgaaggtcgg
     1681 tcgagaaaat agccgaagcc gggaac
//
LOCUS       DAWBSW010000288         1700 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_11201_length_1700_cov_2.518541, whole genome shotgun sequence.
ACCESSION   DAWBSW010000288 DAWBSW010000000
VERSION     DAWBSW010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11201_length_1700_cov_2.518541"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          494 a          338 c          466 g          402 t
ORIGIN      
        1 ttctctgaaa agcatttgct cgtttccgtc ggaagacttt cagctcagaa aaatccgatt
       61 tttgccgttg aaataatttc ggagcttgca ataataagag acgatttcaa atacatttgg
      121 gtaggctgcg gcgagcttga aaatgccgca aaggacaggg cggagcagct cggtgttacg
      181 gacaggatta ttttcacggg tgtcagaacc gatgttaaag aaattcttgc ttgctgcgat
      241 tgcttcctta tgccgtcgct ttttgaggga cttccgttct ctcttatcga agcgcaggca
      301 gcgggacttc gctgtattgt ttccgatgtt gttaccaagg aagccgacgt cggattgatt
      361 gattattttt cacttgaaaa cggtgcaaaa aaatgggcgg agttcattaa cagagagcga
      421 gccgagaaaa tcggcggatg aaaacaaact cagacagttt gatatttcac ataccgtgcg
      481 tcagcttgaa gaaatttacg atagataaaa gaaaggagat gcgatatgag cagaaaaaag
      541 tggatagtca aaagcggcga taaagaaaat gcaacgaagc tttccgaaga gcttaacatc
      601 agcccgtatg ctgcgcttat cgcatcgaca agaggaataa acacgtccga ggaagcgaag
      661 gattttttcg gaatggggga gcgtagaagc gttgacccga tggattttcc cgatatgtat
      721 ccggcggtca agagaattca aaaggccctt gatgaatttg agagaattgc gatttacggt
      781 gactatgacg ccgacggagt tacgtcaacc gcattgctgt attcatatct tgaaatgcag
      841 ggcgcggacg tggtttatta tgttccgaac cgccacacag agggctacgg actgagctat
      901 gaggcaattg acaagctcag catgatggga gttaagctca tcatcactgt cgataacgga
      961 ataagcgccg tcgaagaagc aaaatatatc aatgagcttg aaatggagct tattattacg
     1021 gatcaccatc ttccgtcgga caccttgccg caggccgttg cggtcattga tcctcacagg
     1081 gaggattgca atcttgaatt taaagactat gcaggtgtcg gagttgcata taaattgata
     1141 tgtgcacttg agggcgagga gaacggagtc acggacagct tcgcagacct tgtgactatc
     1201 ggtacggttg ccgacgttat gccgcttata aaggaaaacc gcgagcttgt acgcagggga
     1261 gtacaactga ttgccgattc ggacagaatg ggaatacagt cgcttatcga ggctgccggt
     1321 ctttcggaaa gaaggatgac gtcaacaacc gttgcgtttg gcatttgccc gagaatcaat
     1381 gcggcaggac gtatgggctc ggcagacaga gcgataagac tgcttctgag cgaggattac
     1441 gaggaagcaa acgctcttgc acaggaaatt aacgatgaaa atgcaaccag acagcagaca
     1501 gagcaggaga tactcaatca ggctgtcgct cagataaaaa ataatccgaa ttggatttat
     1561 cagaacgtgc ttgttgtcgc aggtgaggga tggcacgacg gagttgtagg cattgttgcg
     1621 tcgagacttg tcgaaaaata cggaaaaccg acgcttgtta taaccgttga cggcgacgac
     1681 gcaaagggat ccggaagaag
//
LOCUS       DAWBSW010000289         1691 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_11290_length_1691_cov_2.671760, whole genome shotgun sequence.
ACCESSION   DAWBSW010000289 DAWBSW010000000
VERSION     DAWBSW010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11290_length_1691_cov_2.671760"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          532 a          377 c          366 g          416 t
ORIGIN      
        1 cgagatttgc tccgtgccga aggtcagcgt gccgtcgccc gtgttcagga tagctcctcg
       61 atgctcgata acgggaacgg atgaaccgaa gtcatatgtc ctgcgcagaa gagcctggat
      121 ttttgccgtc aaaacgttct catcaaaggg ctttgcgata aaatcatctg cacccatgtt
      181 cattgccatg actatgttca tattgtcgga ggccgaagaa atgaaaatta tcggcacttt
      241 tgaaaccttg cgaatttcgc tgcaccaatg gtagccgtta aagaacggga gcattatatc
      301 aagaagaacg atgtgcggcc ggtattcgct gaattcggca agaacgttgc gaaaattttc
      361 gacagtgcga acctcaagtc cccacgcttc ggtctgagcc ttgattcctt cggcaatgcc
      421 cggatcgtcc tcaactatta ggagcttata cataacctca cctccgataa ttatatagta
      481 tattatatca cagcagcgcc aaaatgacaa gaaataatgg taatccatat cacagattac
      541 caatgagaca aaatacgtaa aaagagccga ttgcaccttt gaaagtgcat cggcttttat
      601 tgttttgaca acggattatt tgctttttct tttggaaagg aaggatttca taacctttga
      661 tattaccgaa tagaaggttt tgaaataaat cgcaaccgtg gcgacgacag cgacaatatt
      721 tatcacgaaa agatatttcg gctcaaaata tgtgcaaact gtctggagca caattatcgc
      781 tgtgcctaca accgtctgaa ccggatacat attgaaatcg accagcttct ttgacatgat
      841 cgctcttata ataaagacga tgaaatagct tgcgaaggtt gcaaccgccg caccgtaaat
      901 ctcatacttc ctgataagaa taaagttcaa tataatattt gtaattgcgc cgatcattga
      961 agtgacaaat gaaagtctgc tctttttgaa cacaaggaac gagctgccca tgaatgttga
     1021 aaacgacgag aaaaccatcg cacagcacag gagcggaaca taggcataag ccgagaaata
     1081 ttcatcggtc gcaacgagca atctgactcc gggcttagca attgcaataa cgaagctgca
     1141 gccgaagaac agaatgcctt gaaaagagag ccaactgtcc gagaagaagg ttttatactc
     1201 gtcaacctcg ccttcctttc gctcgctgac ggcagaatac tgccatgcgt cgctgaacgc
     1261 acccgagaga aggacaatag cggtaggaag ctttcctgcc attgtataaa taccgttggc
     1321 ggtgctgtca accatccaat ttatcatgta tctgtcagaa acggaggtta tccaccagaa
     1381 aatagccgtc ggaataagag gaatactgta tcttagcatt cgtttgataa gacccttgtc
     1441 aaatctgaaa gtaatatatc gccacagctt ttctctgagc gtaatataaa tcgcacatat
     1501 catatcggaa ataacgattg agtaaacata accgtcaatt ccgagtccga gaacggcaag
     1561 gaaaacaatg ttcagtgcaa cgaccgaaaa agtgttaact atgccctgaa ccgcaaagag
     1621 ccttgtgttg cccaagccgc gggcaaactg actgcatatc gagtgaatac acgacgaaat
     1681 ggtataaaga a
//
LOCUS       DAWBSW010000290         1690 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_11298_length_1690_cov_2.120489, whole genome shotgun sequence.
ACCESSION   DAWBSW010000290 DAWBSW010000000
VERSION     DAWBSW010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_1690_cov_2.120489"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          512 a          335 c          384 g          459 t
ORIGIN      
        1 cctttgcatc aattgtatgt tcaaaagtac ccataagagt atttccgacc aagcttctca
       61 ccgcctttcc tcattgcaat gttaaaagtt tcgtgtcgaa atatcccaat tctgcgaagt
      121 ttgagatgtt ttcttaaagt tttgcaccta tgatgttgaa aagttttcaa cactatgtgg
      181 aaaactatgt ggaaagtgtg gataactgcc tttgaaaacg ttgattttac gtatttttcc
      241 acatttttac ccgtggaaaa cacggttttt gttaattatt taacaattgc agttggactt
      301 tgtcaagtat ttttttgtta taaaaagtgg gaatttttgc tcaaatttta ataaatcttt
      361 gtgcaaaaca gagtttaaaa aaagcttgac aagaactttg tgacaaaagg ttacaaaatg
      421 aatatttttt caaagactaa acttttcatt gtattaacgc aaaggaggcc ggcatgaaaa
      481 gctacattaa agatgacagc atagttcttg acggagttga caacctcaat attgccctga
      541 cccttgactg cggacaagcc ttccgctggg cacaaaagcc gaacggagct tggcacggcg
      601 tcgcagaaaa gcgagcagtc gatataattc aggacggaga caaaataacc gttatcggtg
      661 acaccgatga aaacttctgg cagacatgtt ttgatctcga ccgtgattac ggtgcaattt
      721 gcgaaaagct ggagacagac agtcaactca gaagagcgat aagcgaatac ggcggaatac
      781 gaattctccg tcagaacaca tgggaggcgc tttgctcatt tataatctcg caaaacaata
      841 atatcccgag aataaaggga attatagaaa ggctttgtgc aaccttcggt gacgatctcg
      901 gaaacggcga tttcagtttt ccgaccgccg aagtgcttgc aaaatgcaac gttgacgatc
      961 ttgcgccttt gcgttcgggt ttcagagcga aatacataat cgacgccgct caaaaggttg
     1021 cctgcggtga ggtaaatctt catatgcttg ccacggagga tatggatgtc tgccgagccg
     1081 agctgataaa aatcaaggga gtcggtgcaa aggttgccga atgtactctt ctctatggct
     1141 gcggaagaat cgaggctttt cccgttgatg tatgggtcaa gcgcataatg gccgagatgt
     1201 atcccgacgg tttacccgaa tgtacctcgg gtgttgaagg aatagcacag caatttctat
     1261 tccactggcg aagaaatacg gaaataatat aattaacata aacagggaga caatatgaaa
     1321 gatattataa agtatttgcg gcattatatt aaagaaagca tccttgcgcc gctgttcaaa
     1381 atgcttgaag cgagctttga actgtttgtt ccgctggtaa tggcggcgat aatagacaca
     1441 ggcatcaaaa actcggacaa gtcatacatt tttaaaatgg gaatggttct tgtcggattg
     1501 gcgattgtag gcttcatcag tgcgctgact gctcaatatt tcgccgccaa agcggcagtc
     1561 ggattcggca aggagctgag aggtgacctt taccgtcaca tcaacacgct gtcctattcc
     1621 gaaatcgaca aaatcggaac atcaacgctt atcacccgtt tgacggcgga tgtaaatcaa
     1681 atgcaaacag
//
LOCUS       DAWBSW010000291         1615 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_11989_length_1615_cov_1.942308, whole genome shotgun sequence.
ACCESSION   DAWBSW010000291 DAWBSW010000000
VERSION     DAWBSW010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11989_length_1615_cov_1.942308"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1262..1276
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          404 a          376 c          302 g          518 t
ORIGIN      
        1 gattcgaacc ctcgaacagg ctttgacctg ttacacgatt tccaatcgtg ctccttcggc
       61 cagctcggac aactctccaa attctttttc actatttaat tgatgttaca cgattggaaa
      121 tcgtgctcct acatagcttt aatattatac ccgaacgtaa agtgaaaatc aagtgttttt
      181 tcaaattttt cactcgatat caaaatgttt cttccattct gcctcacgga agccgatcag
      241 gacggtgttg ccgtcaataa taagcggtct ttttaccagc attccgtccg ttgcaagcag
      301 gtcaattttc tcctcgtcgg tcatggtcgg aagcttctct ttaagattca gcgacttgta
      361 gagcatgccg cttgtgttaa acagcttttt aatctcaaca ccgcttgcct tgacccacgc
      421 cttaagctca tcggcggtcg gattttcctc tttaatatgt ctgtcaacat aatcaatgtt
      481 gtgctctccg agccacttct tcgccttttg gcaggttgag catttaggat attcaataaa
      541 aatcatagac atacctcact tatcatagct ttccatgaat gaatgaagct cgccgccgtc
      601 gcagtcacgg taaccgatta tcgttgcaag gttgacattg tgaacacttt taaaaatgtt
      661 catgtccacg ttgtcgtttt cctccttgag ctttttaatg agcattttga cctcctggcg
      721 tttggagccg ttatcggctt cctcattcgc ccgggtaacc ttgcaggcac agcggataaa
      781 ttcaagctca ttgtatctcg cccatgagat tatatccttt tctctgacca tataaagcgg
      841 tcggataagc tccatacctt cgtaattcgt acttttgagc ttcggcatca tcgtttttat
      901 ctccgcaccg tagagcatac tcatcagcgt tgtttcgata gcgtcgtcaa aatgatgacc
      961 gagtgcaatt ttgttgcaac caagatcctg cgcaaattta tagaggtatc ctctcctcat
     1021 tcgggcgcaa aggtaacacg gagaaccgcc ctctgcggca tagaccgttt caaaaatcgg
     1081 cgaatcgtaa atccttatcg gaatatccat aatttcggca ttttcaatta tcttcgccct
     1141 gttgttgccg acgtagcccg ggtccatgac aatgtattca acatcaaaat gaaaatcgct
     1201 gtatttctgc aaatgctgca tacacttggc aaggagcatt gaatccttgc cgccggaaat
     1261 gnnnnnnnnn nnnnnncaga cggcaatttt atcgtcaggc tcaatcagct tgtaatcctt
     1321 tatcgctccg acgaaattat tccatattga ttttctgtat ttcttaatta tactgcgctc
     1381 gattagctga tgtctttcca taaatcgctc cgaaaaaata atatatctaa ttttatcatt
     1441 caaaacacta tttttcaaca gctttggtaa agtttttagg ttgaaaaagt tctcttttta
     1501 atgtataatg tcaacatata tacgaatatc ggagttgatt ttttgaataa agcatcggaa
     1561 tacagggtca tgccgaccgt tgccctcagg ggtttggttg ttttccccgg tatgg
//
LOCUS       DAWBSW010000292         1607 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12058_length_1607_cov_2.095361, whole genome shotgun sequence.
ACCESSION   DAWBSW010000292 DAWBSW010000000
VERSION     DAWBSW010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12058_length_1607_cov_2.095361"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          369 c          339 g          472 t
ORIGIN      
        1 cgtcagagcc ttgaactcaa attgcttctt ttcgtcaagc tccttcggct caataaatcc
       61 cttggggaaa tgcttgtaaa cagcggctat cgcatcgccg tcacgggtgt cgcttgcggt
      121 tataactacg caggcgtcct gatcggggaa catgaaagaa aattgtccgt agagtcccgt
      181 aaagcgatag gtcgtatcgt gatgctcatg gtcgatccaa acctgataac cgtattcggc
      241 attgtcggag aataccggat aagtcttttt ggtgtacgga taagtcgctt ttttaaacca
      301 atcttcggag aaaatccgct taccgttgta gactcccttg ttgatataaa gcagtgaaat
      361 tttagcaatg tcctcggctg tgagctttaa gccccagccg ccggcgtcaa catctacctg
      421 gcttttttcc caatgcggaa tcttgatatc aagcggctca tacaatctcg gagtgagata
      481 ttccgtcatc gtcattcctg tttctctttg caaaatccat gagagaagat aagcattctc
      541 gctaatgtat ttccaatcgc tgttcggctc aaactgaatc ggtttgtcaa gaattccgtt
      601 gacccagtcc gtctttgctt tatcgtcaaa aacgcttgtg accttgccca ttctcatgga
      661 aagtacatca tggaccgtaa ccttcttagc ccattcaagc tgtttgccgg tcagcccgtc
      721 aagcttgtgc ggaaaatatt tttcatatat cctcgtgtca agtgaaatat agccttcatc
      781 aacggcaatg cctatcgccg tagcagttac gcttttgctc agggaataca tatcatgcgg
      841 cgtatcgact ctgttcggtg cccagtgaca ttcgcaggca accttgccgt gccttataac
      901 catgaggctg tctatgttat atacaagctt ttcaaagaat ttaatcaaat cggaaacaac
      961 gtcggaatca acgccaacct gctcgggata atcggctctg tcaagcatta aaatctctcc
     1021 ttgttttttt caaatattat agcatgattt gtatataaaa tcaaagaaaa aattgcagat
     1081 gaattactgt ttgtgtaaat tgtccaagta attatttact gattttctca aattcaacat
     1141 tttttccgtg ccgcgcggat agtcaaaaaa tgttattatg ccgaaatcgg cttcaactgc
     1201 agcaatagtt ctttttttgc cgattctgtc gcatagaagc gtcagagcac gcatatcctg
     1261 caatgcttga aagaaaacct cactgcgaac agattcaatc gcaccgcttt ttccggggta
     1321 aacggtaaat gaatcgcccg acggaaatgc cgagtctgcg tctgtaaccg caaacgggtc
     1381 gatgcttctc agggagtact gggagttgta gaaattaaag ccccattgta aaaagccttc
     1441 aatcccgtat ttaaaaagct gaaatcctat aattcttgtt gtcgagagcg gcattccgaa
     1501 aaagcgattg ctgagcttgc cgccctgacc gcagcaataa tatgtccacg ggtgtgggaa
     1561 gcctttttta ataaagcttt caatatcgtt tgtgtacggt atcggat
//
LOCUS       DAWBSW010000293         1601 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12115_length_1601_cov_2.773609, whole genome shotgun sequence.
ACCESSION   DAWBSW010000293 DAWBSW010000000
VERSION     DAWBSW010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12115_length_1601_cov_2.773609"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          400 a          401 c          418 g          382 t
ORIGIN      
        1 atcaataaca tagccaccga gcacggcggc atttccgttt tagccggcgt cggcgaacgc
       61 acccgagagg gaaacgacct ctattacgag atgaaagaat ccggcgttct caacaagacc
      121 gcacttgttt acggccagat gaacgagccg ccgggagcga gaatgagagt tggtctttcg
      181 ggacttacca tggcggaata tttccgtgac gaggagaagc aggacgttct tcttttcatt
      241 gacaatatat tcagatttac acaggcaggc agcgaggttt ctgcactgct cggacgtatg
      301 ccctccgccg tcggatatca gccgaccttg gcaacggaaa tgggcgcatt gcaggagcgt
      361 atcacctcaa cgaagaaggg ctcgatcaca tcggttcagg cagtttatgt tccggccgac
      421 gacctgacag accctgctcc ggcaacgacc tttgctcatc ttgacgcgac aacggttctt
      481 tcaagaagta tttcttcgca gggtatctat cctgcagttg atccgcttga atcaacaagc
      541 cgaatcctca atcccgagac tctcggcgag gagcactacg cagttgtaag agaggttcag
      601 cgtgttcttc agcgctataa cgagcttatg gatatcatag ccattatggg tatggacgag
      661 ctttcggatg atgataagct gcttgtaagc cgcgcgagaa aggttcagag attcctttct
      721 cagccgttcc atgtttcaga gaagtttaca ggcttcccgg gaatttatgt tccacgctcg
      781 gagacaatca ggggcttcaa ggaaatcctt gaaggcaagc acgacgatct tcccgaatcg
      841 gcattcctct ttgtcggaac gattgacgat gctgttgaaa aggcaaagag gttatcgcaa
      901 tgaatacttt caaactgacg atagcttcgc cggacggaaa caggttttcc gacgaggcac
      961 agatgctaac cgtgagagga attgaaggcg acttggcgat aatggcaggc catataccgt
     1021 ttatcaccgc attgcagcca tgcgactgta aggtagtcct tcccgacgga acggaaaaaa
     1081 tcggagcagt cgacggcggc ttgctcaccg tctcggaaaa aggcgcaacc ctcctttccg
     1141 gcagcttcca atggaaataa tagaaatata actaccggac tgtgacggct tttgagtcac
     1201 agcccggttt ttctgtgtat gaatataaaa aatcatcctc ccaatgaaca gggaggatga
     1261 tgaattttta ttaaagcagg ttgatgccgc tgtttttgca gtctgcaagc attttttcgg
     1321 gccagaggga tacctgaacc tcaccgatgt gagctttttc aagtatgagc atgctcagcc
     1381 ttgactgacc gatgccgccg ccgattgtca gcggaagcgt gccgtcggca atgcccttgt
     1441 ggtagtcata cttcattctg tcctcggcgt ccgccgcctt cagctgataa gcgtgactct
     1501 cggaatcaac acgaataccc attgaggaaa cctcgaatgc gtgccggagc gtttcgttcc
     1561 agaaaagcag gtcgccgttg agcttccagt cgtcgtagtc g
//
LOCUS       DAWBSW010000294         1597 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12162_length_1597_cov_2.543450, whole genome shotgun sequence.
ACCESSION   DAWBSW010000294 DAWBSW010000000
VERSION     DAWBSW010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12162_length_1597_cov_2.543450"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1003..1012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          467 a          312 c          415 g          393 t
ORIGIN      
        1 cggcggattt accgatcccg aaaagaacgt ttggaaggtt gaaaaatcaa agagaattct
       61 tgaccttgcc aaggagctcg gaacggacgt tgttacaact catatcggcg ttgttccgga
      121 ggatgagaat accgacagat ttaagataat gcaggaggct tgctttgagc tgagccgcta
      181 tgcggacgac atcggcgctc attttgcaat tgaaacaggt cccgaaaaat cggctacact
      241 taaaagattt cttgactcgc tcggctcaac gggtgtaggc gttaatcttg acccggctaa
      301 tcttgttatg gttacaggag acgatccggc gggggctgta cataatttaa aggattacat
      361 tgttcatact cacgccaagg acggcaaaca gaactactat gtcgatcccg atattatcta
      421 cggcatggtt gaatcccata tcgtgacggc ggacagcttc attgaggttc cgctcggcga
      481 aggcagcgtt aactgggatg aatatctcaa ggctcttgag gatatcggct ataagggctt
      541 cctcaccatt gaaagagagg tcggcgacga tcccgaaaag gatataagaa aggctgttga
      601 attcctcaac ggtaaaatgg gtgcttaaaa tgaagatttc tgtcagcagc tacagctatt
      661 ctcagctcgg gaacagtgac tttgaaagaa tcgaccttgc caaagagatg ggctttgagg
      721 gcattgaatt tgccgaaatc aaccccggag agaagaataa aatcgaatat gccaaggagc
      781 ttcgtgccta tgccgatgaa aagggtgtaa tgatttcaaa ttatgccatc ggtgcggatt
      841 ttataaacag aaatgttgat tccgagattg accgcctcaa gggtgaggtg aacattgccg
      901 agattctcgg tgtaaaaaca atgcgccatg atgcgactat cggacccgaa gccgaaagat
      961 tttccaagac aggctttgaa aaatatttgt ctgtgcttgc aannnnnnnn nnaagggctg
     1021 taaggcggtc acggaatacg caaagacaaa gggcattaaa acgtcagttg aaaatcacgg
     1081 attttactgt cagcagagcg acagggtaga ggcacttata aatgcagtca atgacgataa
     1141 tttcggctgg cttgttgata taggcaattt cctttgtgca gacgaaatgc cgcttgatgc
     1201 cgtaaaggtc ggcgtgaagc atattgttta tgcgcacgtt aaggattttt actttgccga
     1261 taaaagcgtt ccgactccag agggatattt tgacacgaga gccggcaacc atctttgcgg
     1321 ttcgattctc ggcgacggaa ttgttcccgt taaggaatgt gttgagctta taaaaaattc
     1381 gggctacgac ggctgggtta ctctcgaata tgagggcagg gagagcccgt tgacggctgt
     1441 gccgaaaggc ttggaatatt taaaaaaatt agtataaaag gatatgattt tgttgagcga
     1501 aaaaagaatt gaaactaaat gtgttcaggg cggctatact ccgggtaacg gtgagccgag
     1561 acagattccc ataatccaga gcaccacata taaatac
//
LOCUS       DAWBSW010000295         1588 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12253_length_1588_cov_2.696673, whole genome shotgun sequence.
ACCESSION   DAWBSW010000295 DAWBSW010000000
VERSION     DAWBSW010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12253_length_1588_cov_2.696673"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          402 a          370 c          399 g          417 t
ORIGIN      
        1 gtcttgtaat cggcgcaggt atggaggatc tcggtaaggg tgctcgctcc atggtcggca
       61 caatgtacgg cacaaaggca aagggcgttc gttatcttga gatgacagag ggctattgca
      121 caagaatggc tcttgacgct gacgatcagg ttatcggtta tgaattcgtt tctctcggca
      181 agatgaccga cttcatcaag aagggcatgg agccgaacga ggcatatgag aaatcaaagg
      241 gcacatacgg ccgcttcgac gaagctgtta agtatatcga cccgagaaaa gaataattga
      301 aggaggaata acgcaatggc acagtttgaa ggttatgaga gacgtgaggc taagatcctc
      361 ggcgttctta aagagtacgg tatttcctcc atcgacgaat gtaaggaaat cacacttgca
      421 aagggtatcg acgttgacca gatcgttcgt tcaactcagc ccatttgctt tgaaaatgct
      481 atctgggctt acactgtagg cgcagctatc gctatcaaga tgggttgcac aaaggctgcc
      541 gacgccgctg ccgctatcgg tatcggtctt cagagcttct gtattcccgg ttctgttgcc
      601 gagaaccgta aggttggtct cggtcacggt aatctcggta agagacttct ttcagaggag
      661 acagagtgct tcgcattcct cgcaggccac gagtcgttcg ctgctgccga gggtgctatc
      721 aagatcgctc ttaatgctaa caaggttaga gttaagccgc ttcgtgttat ccttaacggt
      781 ctcggtaagg acgcagcaat gatcatctcc agaatcaacg gcttcacata cgttgagaca
      841 gagtttgacc cgtacacgga agaggttaag gttgtttacg agaaggcata ttcaaacggt
      901 ccgagagccg atgttaagtg ctacggcgct gacaacgttc ccgagggcgt tgctatcatg
      961 aagcttgaga atgtcggcgt ttcaatcaca ggtaactcaa ccaacccgac tcgtttccag
     1021 catcccgttg caggcacata caagaagtgg tgcgttgaga acggcgttaa gtatttctct
     1081 gttgcttcgg gcggcggcac aggccgtaca cttcacccgg acaacatggc tgcaggcccg
     1141 tcttcatacg gtcttacaga tacaatgggt cgtatgcatt ccgacgctca gttcgctggt
     1201 tcttcttcgg ttcccgctca cgttgagatg atgggtctta tcggtatggg caacaacccg
     1261 atggttggtg ctaccgtcgc aaccgctgtt gcagttgaag aggcaatgaa gtaagccgca
     1321 aagccttata aatcaacact ttcagagctt cgctcacttc gagcgaggct ctttttctgt
     1381 ctcaaaatcg acgtttcaaa atgccctcgg tcctcatttg tcctcataat tcgtattttg
     1441 tcctcttttt ttgtcccgaa ttttgaggac agaacagctt tttcgccgca taaaaaatat
     1501 tcccgccgaa aaaaccacat tttcggcagg aatgaatatt gatgtatatt tttaatatct
     1561 aaatcattct ttatatcaag cactataa
//
LOCUS       DAWBSW010000296         1586 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12284_length_1586_cov_2.536904, whole genome shotgun sequence.
ACCESSION   DAWBSW010000296 DAWBSW010000000
VERSION     DAWBSW010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12284_length_1586_cov_2.536904"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          443 a          346 c          377 g          420 t
ORIGIN      
        1 cgatgtacag tgaattctca aagctgtgct ttgcaagcat tttcttaata tttttgccct
       61 ttgaaagcgt ttcgggaccc tcaaaagcag tgtcgcagaa atattttccg agtttatgat
      121 atgaaagaaa agcgtccatg tagctctctt gacagttgct gacaacgtac agcttgaaat
      181 gtttgctgag ttctgcaaga gtttcctcca cgccgtcata aagaattccg ccgcgctgtc
      241 ttaattcttc aacgcacgca accgttgcat cgttaaagaa ttgctccgcc aaagctttat
      301 ccgctctcgg gaaaagcttg ttcatcaggt caattttatt aagccccata accgaacgga
      361 tctgttcgtc ggtcgggagg ggaaacttat atttctcggc tacactctgc catgacgatg
      421 ttatacagtt catggagctc caaagcgttc cgtcaagatc aaatccaatg ctgtcaaaca
      481 taaatagcac ctccgtatat acaaatataa tacaggtatt tttttacggt gtcaaataat
      541 aaattaaata attacataaa aatgttgaca aaccttgacc cttgtggtaa aattctaaat
      601 accgcatatt acgggcgtcg ctttcgggcg gcacaagtga tttattccgc cccacgtagc
      661 gagtccataa taaaggaagg ttagttagaa tgtacgcaat catcgttacc ggcggcaagc
      721 agtacaaggt tgaggctgga gacacccttt acattgagaa gcttgacgtt gaggctgaca
      781 gcgaagttgt ttttgacaag gttatcgcag tcggcaagga agatggcatt gttgtaggcg
      841 ctccgtatgt tgacggtgca actgtaacgg caaaggctgt caagaatggc aaggctaaga
      901 aggtagttgt atttacctac aagccgaaga agaacgagaa gcgcaagatg ggtcacagac
      961 agccctacac taaggtagag atctcagcta tcaacgcata agcaatgatt aagattgatt
     1021 tcttcttttc ggataaagca atcgagggct tcaggattag tggccattca ggttcggcag
     1081 aggccggttt ggacattatt tgttcgactg tttcctcggc ggcctacatg gcggcgaaca
     1141 cgattaccga tattgttaag tgcaaggcgg aggccgatgt ttccgacgga ttcatgagct
     1201 ttcacgtcaa gtcgaaatta tccgatgcgc agcaaattct tgagggctta aagcttcacc
     1261 ttgaggcttt agccgcagat tatccagaca acattaaagt aacactacgg aggtgtcaaa
     1321 atgcttaaga tcaacattca gcttttcgct cataagaaag gtatgggttc cacccgtaac
     1381 ggccgtgatt ccgagtcaaa gagacttggc gcaaagcgtg ccgacggtca gtttgttctt
     1441 gcaggtaaca tcctttacaa gcagcgcgga acacacatcc atccgggcaa caacgttggc
     1501 aagggttctg acgattctct tttcgctctc atcgacggta ctgttaagta cgagcgttgg
     1561 ggcaaggatc gcaagaaggt cagcgt
//
LOCUS       DAWBSW010000297         1579 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12367_length_1579_cov_2.176509, whole genome shotgun sequence.
ACCESSION   DAWBSW010000297 DAWBSW010000000
VERSION     DAWBSW010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12367_length_1579_cov_2.176509"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          473 a          365 c          364 g          377 t
ORIGIN      
        1 ataaacaaca gcttcaaagg attgattgta tgagagatat agaaaaagtt cgccgctttt
       61 ttgagggaga cagatatgcc gttgaggtta cgggcgtaaa aatcgacgag gttgatgact
      121 gctattcaaa atgcagtctc gaccttgata aacggcacta cggagcacac ggtcaagtca
      181 tgggcggagt tatgttcact cttgccgact ttgctttcgc cgttgcaacc aatacttccg
      241 agagcttcac cgccacggca gacagccaca tcagctatct gacatcatca aagggtaaga
      301 agcttttcgc cgagtgcaga aaagtcaagg agggcagaaa gctctgcttc tatgaaataa
      361 cgataacaga cgatctcggt gccgaaattg ccgttatcag cacgaccgga tttcacatcg
      421 gagattaacg aggtaaaagc atgacacaga cttttttatg tatcataaca tcaatcttga
      481 tgttggttgc cccgattttc aatcttaaac cgactccaat tactatgccg gagaatctcg
      541 ctgccgaaag cgagccgaca acagatgaga tgcgcaccta cgaccgagtt gacggtgtaa
      601 atgattccgt tttctatatt gcaagaccga acgagaaaaa ctataagacc gtcaacgctt
      661 cggatttcgg cgtgagcaca gcggcagatg acaactattc ggcattttgc aaggcgattg
      721 aatattgcaa ggccaacccg aatattacgc tcaaggttga cggcggtaaa tactatttcc
      781 gaaccgacaa agcaatcatg ctcgacggcc ttaaaaacgt tctcattgat gccgacggag
      841 cacagttcat ttttgagaac ccgaattatt ttcatattgt aaactgcaac tgcattgaga
      901 tccgaggtct cggcataaca tggaaccttg atgtttcaag actcgcctcg cttgtgaaga
      961 taagaaatgc gagcaaagag tctcaaagct ttgagcttga atttaccgag cttaatgagg
     1021 tttcggcaga tatcccgata atggcgttta caaagtatga ccctgagacc ctcactcccg
     1081 gaacaaggca ggatttcaaa gaaaactatg tgtaccaatt ccccggcagc attaaaaccg
     1141 ttgaaaaaac cgggcacaac gtgctgaaag tcacacacga cggctcactt gataattata
     1201 cggacggtga ggtttatctt ctgcgccacc atgtttacgg cggcaatgca ttcaatgtat
     1261 acaacacgtc gaacatcact ttttcaggca ttaagcttta tgcggttgca ggcatgggct
     1321 ggctgattga aaatcgctcg gagcactttc aactgctcgg ctgtgtgatc ggacttgacc
     1381 ccgagcacga cgataacaga atttcgacga ccgccgacgg cgtgcacatc gcaaacacaa
     1441 acggtaaatt cagaatcagc ggctgtgatt tcagcttcat gggcgacgat gacgttaacg
     1501 tacacgacaa cattgcaacg gtaacagaca agctcgatga aaaaacggtt gagatttaca
     1561 ccaatgcaaa caattttgc
//
LOCUS       DAWBSW010000298         1533 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_12861_length_1533_cov_2.288227, whole genome shotgun sequence.
ACCESSION   DAWBSW010000298 DAWBSW010000000
VERSION     DAWBSW010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12861_length_1533_cov_2.288227"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          465 a          363 c          361 g          344 t
ORIGIN      
        1 atgctacact cccggtatgg gcggcatcgc cgaagcagtt atgaaaatgt gattcggtaa
       61 cggactcggc ttcagctaca gcgaaaagga agatatgaat aaaattttcg gctacagcta
      121 tgccggcttt attcttgagg tcgcagactg ggacaacggg gacgaaataa tcggaaccgt
      181 taccgaaaaa tgtgatatat catacctcgg tgacaccgtg tcgcttgatt ccctgctgaa
      241 aatttacgag aataagttag aaagcgtttt cacgtgcaac attaaaaaca caggcagtaa
      301 gcccgaaaac gtttcatata ctgccacaga gcgcacagcg ccgtcaatca aggtagctaa
      361 gccgcgtgtt ctcattcccg ttttcccggg tacaaactgc gaatatgatt ccgcaaaggc
      421 tgtcgcaaac gcaggcgcag agccggtaat cacggttatc aataatctct ctgcagacgg
      481 aatcgcaaga agcattgaat gctttgcaaa ggagcttaaa aattctcaga tcgtattcat
      541 tcccggagga ttttcaggcg gagatgagcc ggacggaagc ggtaaattca ttactgcatt
      601 tttccgcaat cccgaaataa aagagggcgt tacagagctt cttgagcacc gtgacggtct
      661 tatgtgcggc atctgcaacg gtttccaggc tcttattaag ctcggacttg tgccgtacgg
      721 aaaaatcatt gacacggacg agaattgccc gactcttaca ttcaacacaa ttgcaagaca
      781 tcagtcaaga atcgttcgca caagaatcgc ttcaaacaag tcaccgtggt tatccgaaat
      841 gaacgtcggc gatattgtta atgttccgat ttctcacggc gaaggccgtt tcctcgccaa
      901 tgacgaaacg atacgcagtc tgattgaaaa cggacagatc gcaactcagt atgtcgattc
      961 aaacggcaat gcgtcaaatg atatccgctt taatccgaac gactccgcat acgcaatcga
     1021 gggcattacc tcccccgacg gcagagtttt cggaaaaatg ggtcacagcg aaagaatcgg
     1081 agccggactt tataaaaatg ttccgggcaa ttataacata catatgtttg aatcagccgt
     1141 aaaatatttt aacaagtgag gagtatagcc atgtcacaac tttccgatat tgaaatagct
     1201 caacagtgca agccaaggca cattacagaa attgcaaaaa aagcacacat tgatgaaaaa
     1261 tacatcgaac agtacggaaa ctataaagcc aaaatcgatc cgcagctttt aaaggaaaca
     1321 gacaggaaaa acggcaagct aatacttgtt accgctatta cccccacccc cgccggagag
     1381 ggcaagacaa cgacaacaat cggtctggca gacggtctcg cccgtctggg caaggatgtt
     1441 acggttgcgc tgcgtgaacc gtcattgggt cccgttttcg gcataaaagg cggtgccgcc
     1501 ggcggcggat atgcacaggt cattcccatg gag
//
LOCUS       DAWBSW010000299         1516 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_13089_length_1516_cov_1.739220, whole genome shotgun sequence.
ACCESSION   DAWBSW010000299 DAWBSW010000000
VERSION     DAWBSW010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13089_length_1516_cov_1.739220"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    268..277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    815..824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1156..1165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          487 a          282 c          297 g          420 t
ORIGIN      
        1 atccagcaga tgcaggaaga tatgacaaga attcaggagg agattgaagc aactgaatat
       61 acttcttccg taggcggagg agctgttgaa gttacagtaa acggctctca tgaagttctt
      121 tcaataaaga tgcagcccga cgttgttgat ccggaggata ttgaaatgct tgaggatctt
      181 cttatttcag cacttaacga gagcattaag aaagcaaacg atgccatgga tcagggcatg
      241 gaaaaagcaa agggcggact ttcaattnnn nnnnnnnttc tcggaatgtc tgatgatgag
      301 gtaaaacgct ttgcggaaaa tattatcctt gcaaagcaaa aaattcatca ttgcaaggtc
      361 tgctgtgatt tgacagacag cgatatttgc tcaatctgca gtgacgaacg cagagaccgt
      421 tcggttattt gcgttgtcga atcttcaaag gatgttatcg caattgagcg cacccgtgaa
      481 tataacgggc tttatcatgt tcttcacggt tcaatttctc cgatgaacgg catcggaccc
      541 gatgaaatta caatcaagga gcttctctcc cgtctttccg atgaaaacat aaaggaaatt
      601 attatggcaa caaatccgac tgttgagggc gaagcgacag caatgtatat ttcacgtctt
      661 attaagccta tgggtattaa agtctcacgc cttgcatacg gtgtgccggt cggtgccgac
      721 cttgaatatg ctgacgaaat aacgttaaca agagcgcttg aaggcagaca aatattgtaa
      781 aagttttcca cactttcttt aacattcttt caacnnnnnn nnnnttcaac agctttcaac
      841 ttttcaacac cgttatttaa ttaacaaaat tttcaacaaa aaaataaaca cattttcaac
      901 aagaaatatt atccttaatc gacgttgttt caacaaattt tcttgttttc cacactttta
      961 acaggcccta ctactaatac tataatattt atattattta tttatcttaa aacccgacag
     1021 gaggaatttt aatgaaattc gtatgcgaaa cgtcaagtct ttcggtagca tgccaaaatg
     1081 tacaaagagc ggtatcaaca aagtcatcta ttcccggagc ggagggtatc atgatcaaga
     1141 ctaccgttaa cggacnnnnn nnnnnctttc gctgacaggc tatgatcttg aaatcggaat
     1201 caacacaaat atttatgcaa aggttgagga agagggagca attattatta acgcaaagat
     1261 tctttgcgag atactccgta aaattccaaa tcaaaccgta atatttgaat ctgacgaaag
     1321 aaatcttgcc gttatcagaa gcggtgacat taaatatgaa ataatgggaa tttctgccga
     1381 agaatatccc gagcttccta cggttcaggg cggatttaca ataaatctta acaagaagca
     1441 gcttgaagaa atggttaaac aaactatttt tgccgttgcc gttacagaca caaaagttgt
     1501 ttatacaggt attaag
//
LOCUS       DAWBSW010000300         1515 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Acutalibacteraceae bacterium isolate gSpreadC_ID_1681
            NODE_13100_length_1515_cov_1.846575, whole genome shotgun sequence.
ACCESSION   DAWBSW010000300 DAWBSW010000000
VERSION     DAWBSW010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994807
            Sequence Read Archive: SRR3917698
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acutalibacteraceae bacterium (human gut metagenome)
  ORGANISM  Acutalibacteraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Acutalibacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Acutalibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13100_length_1515_cov_1.846575"
                     /isolate="gSpreadC_ID_1681"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:3077420"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    417..507
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          410 a          323 c          344 g          347 t
ORIGIN      
        1 caaaagacgt gatgtacttt tcgaacgttg agccgagaaa atagccgata gtgggcataa
       61 gagcctgaaa tccgccgaac cacgcaccga ttatcaggta gtcctttgtt ttaagcttct
      121 gcgtcgaaag acccttgcag accgaaacgg cgaaagcgtc cattgacaga ccgactgcca
      181 ggataaataa ttcaagtaac cccatatttt acctccgaga ataaaaaaat cagacaccgt
      241 cgtgcctgaa aaaatcccag acacggcaaa gccacgtctg agtctcgtta tttaagacaa
      301 gccagacgct ttgcgtcagt gtgttgactt gtcactcgtt tcgagctatc tactccctca
      361 aactctgccg attatatcat attaaaatgt atgtgtcaac cttcatttct tgaacgnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnttg tttgtcgggg atagagtgtt atttattgtt
      541 aggagaggaa taccgaacga cattgacttt tatcggcaac attattgttg acaaagcagg
      601 ggaaatacat taaactgatt ttaatataaa acaaaggagc tttattatgc tttcaaagcc
      661 tcaatacgaa gaacagatcg ccgcaacccg tgataagcgc atgaagtggt ggcgagaagc
      721 aagattcggc atgtttattc actacggcct tttttcacag ctcggaagaa atgagtgggc
      781 tatggcgtgc gaaaatattc cgcccgagga gtatgaagca cttgccgata atttctgccc
      841 gaaagagggc tgctgccgtg aatgggcaaa gcttgccaag gaagccggtt gtaaatatat
      901 ggtcatgacg acccgtcatc acgagggctt cagcctgtgg aattcaaagg caaatccgta
      961 caattccgtc aattacggtc cgcaccgcga tatagtcaag gaatttgttg aagcatgccg
     1021 tgagtttgac ctgaaaatcg gtttttattc gacctgcatg gaatggcatc acccggacag
     1081 cggaatttgt gcctatgaca cagaggcgag aaggcgttac actcagttcc ttaaagatat
     1141 aaacacagag cttctcactc agtacggcaa gattgatatt ctttggtacg acgttccgct
     1201 gccgatggag tcatgggagg gttgggattc gcttgagcgc aaccagtatc tcagaagtct
     1261 ccagccggat attattatca acgacagaag cggacttgcc gaggatttcg gcactccgga
     1321 ggagcacatc actccgtccg agcgtgactg ggaggcttgc atgaccttca acggaataag
     1381 ctggggctat gttgattcgg atcaggcact cccttacagc tacacgccgc agagaattgt
     1441 caatatgctt caaacctgct gcgaatgctc atgtacggta aggaagcaag caaccgaaaa
     1501 caagagatag accgc
//
