LOCUS       DAWCYS010000001        38141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_51_length_38141_cov_10.559733, whole genome shotgun sequence.
ACCESSION   DAWCYS010000001 DAWCYS010000000
VERSION     DAWCYS010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 38141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 38141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38141
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51_length_38141_cov_10.559733"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8082 a        10444 c        11261 g         8354 t
ORIGIN      
        1 ggcccgcctg aaatgtgatg tacacgccat acatgacggg ctcggactca gatgcggcgt
       61 tccgccgcga cagactcaaa ctgacaacga cgcttcggga aaccctcagc gcgtagcggg
      121 cgccccacgt ggcgccccta cgatgcggct gcgcagcgat aaactcaaac taacaacatc
      181 gcttcgagta accctcagct cgtaaaaacg ccccgaccgg ggcctgcgta ggggccgatc
      241 tttaacctct cagaccgcct ttagcggtca gctcccctat aaagggagcc ttgggcggcc
      301 cgccgcgaat aaatggtgat atacataaca ggtccatgag gcatggtttt gcgaactgcg
      361 gcctgcgaat ggcgttttct ccgaacagca tttcgcggga ttctcacaac gggacgccta
      421 gccacagctg tccgttttgg cagccgtcct gcgcctcctg ctggtcggca cctgacgggc
      481 ccaaaaggct tacaggatgt cccctacgaa agcggtccgg tggaccgtct gctaactgcg
      541 atcggcagcc tgcgaataat gttttctttg aacgccgttc tacagcaatt cgtatttttg
      601 gtttttttga tagcttatca tgaatacccg gcccttttcg tcgaacatga tggtcaggta
      661 atagtccggg tagacgacgt cggacaggag gtattcgtta ccgatggcct tgagttcata
      721 catgaatttg aaggctggac gcagcttttt cagggcgtcg ctttttttgt cgccaatctg
      781 caggccgtag aaagaatgag ggactttgtc atcagcactg atgatgaggt cttttttttc
      841 ttggcgactg taatacaggt cctgccatac cgttgctggc ttggcttgtt gtacatcctc
      901 ttcctggccc agtgccaggc cgggaagcca caataggcct aagagtccgg ctgcgataag
      961 acgatttttt ttcataaaga cacctccgca ttcagcatag gccgggcgga ggtgttttgt
     1021 caaggcaggt gtcatccatt ttgacagcta tgcgttttgc agcgatattt ttcgggaagc
     1081 tggacgatga tgatgcccag gaggatacag atggctccgg ctaattggac gggcgtaaag
     1141 gtttcgccca ggaaaatgac gcccaggacg gcggcgatga gcgggttcag tgctgtaaac
     1201 aggccggctt tttcggcgct ggtatacttt tgggcgacgg gctggaaggt gaagccaaag
     1261 acggaacaga tgaagaccag ggccaggatg gcaccccacg tcgtcgtatc ggcaggaacg
     1321 gccgggcttt caaataagac catgcctgct gtactgtaca cggcgaggaa tcccatttgc
     1381 aagatcccca gcagcaatgg gtcgtactgc cgggccaggc gggcggtcag gcagatccaa
     1441 agggcgtaga agacggaacc ggtcaggcag atgatttctc ccggtgccag atggaaaacg
     1501 gcgccttgca gggtaagaca ggcgatgccg atgaggagca ggctcgtagc cgtcaggact
     1561 tgctttcccg gccaatggtg gctgacgacg gccattagga tcaggaccca gacggcggat
     1621 gtattttcca ggaaggccgc tgtcgacgag ggaatcgtcg tcaggctgat gagttcacag
     1681 gccatgcagg cgaagaggaa ggtccctaag aggccggcag ccttccatag ctggcgcgtc
     1741 aacagcggga ggcggcggtg aaagagcagc agcaggataa gtgtcgtcag ggcgaagcgg
     1801 attgccagta attctaaagg cggtatcccg ttcagtccga ttttcgacag gagataggaa
     1861 atgctgcgcg aagcgatgac gatgatgagc agtatttctg catttctttg tttcatgaaa
     1921 aagtccctcc ttactttttt cttgctttta tgatacgata aaagcagtat ggagagaata
     1981 gcaaaataat atatatagtt ttgataaaaa agcaaaggtg atagatatga atacggaaaa
     2041 atgcaggctc ttgctgcaag tcatccaggc gggcagtttt tcttcagctg ccgaacgatt
     2101 gggatatacc ccgtctggcg tcatgcggtc cattgcatcg ctggaaaaag aaatcggctt
     2161 tcccctgctg gggcggacgg cacaaggcat ttatctgacc cgtgaagggc aggcagtgct
     2221 cccttatctg cgccgcctca tccgtgatga agatgctttg ttgcagtaca gtgcccagat
     2281 ccggggattg acggccgggg atatttacat tggctcttat ttcagtgtag ccgccaactg
     2341 gctgccgcag atcatacagg ctttccagca ggcctatccg gcagtccacg tccacatcga
     2401 ggaatgcgcc aataaagtga tgtacgatgg attggaagaa gggcgcttcg attgttgtat
     2461 cacgacgtgg cgcccttttt ccggagactg gattccctta tgtgaagatg aaatggtcat
     2521 gtggcttccg gaaaatcatc ccttggcacg agccgaggta tacccgttga agcagttgtg
     2581 tcatgaatct tttattgaac cactgccgca gcaggatacg gatgtcgtcc ggatccttaa
     2641 ggaagaacat atttcttatg aaacgcgata tacggccatc gataactata cgacctatcg
     2701 catggtcgaa gccggcctgg gtgtcagtat gaacaatcgc ctcatgacgg cacagtggca
     2761 gggccgcgtg gctatccttc ccatggatcc gccccatacg attaccattg gcattgccat
     2821 tcccagccgg gatggggcat caccggcagt caagaaattc atccattacg tgcaacaggg
     2881 aatcagtcag atccttgcgc cccggtaaat gcgggtcccc gtctttccga ggatgccttc
     2941 tttggctttt tggagcagtg tgatcaaggc tgtccggccg ggacgggatt cggcgaattt
     3001 gacggcagcc tggactttgg gcagcatcga accaggggcg aattcatttt gagccatata
     3061 ttgccgggct tggtcggtag tcatttccga cagccactgt tcgttcgcct ggccaaaatg
     3121 gatagcgacc ttttccacgg ctgtcaggat gatgaggtag tcggcatgga ggtcttcggc
     3181 caaacgttca ctggcgaaat ccttgtcgat gacggcgctg gcccctttga ggtggttgcc
     3241 gtggcgcatg acggggatgc cgccgccacc gcaggcgatg accagctggc cggaatcgac
     3301 gagggtctgg atggcgccga tttcaatgat ttcctggggc agcggcgagg cgacgacgcg
     3361 gcgccagccc cggccggcat cttccttgac gatgtagccg tattctttgg cagcgtattt
     3421 cgcctgttcc ttggtcatga aatggccgat gggcttcgac gggttcttga atgccgggtc
     3481 ttccgggtcg acgcggacct gagtgaccat ggtggcgacg gggatgtcga agatgtcgcg
     3541 gttgtacatt tcttcgcgca gggcattctg caggtcatag ccgatgtaag cctggctcat
     3601 ggcgacacag accgacagcg gcgtgttggg ctggttcggg tcttcccggc tcagggcggc
     3661 catggcgtgg ttgatcatgc cgacctgcgg gccgttgccg tgggcgacga tgacttcaca
     3721 gccttcctgg atgaggtcga cgatggcttt ggctgtcgtc ttgacggcca gcatctgttc
     3781 gtgcagtgta tttcccaggg cgttgccgcc gagggcaatg acgatacgtt tctttttctc
     3841 ggtcatgata aatctccttt tatgtgtgaa aaagttttat aaatgatgag taaaacgcat
     3901 ggcagggaga tgaaaaaggc accggtccgg aggaccggca cctttggcgg tcaaattatt
     3961 tcattttgcg caggatgccg cgttcttcta aggctttcag ggtctgagcc ggatcctggc
     4021 atttagccag cataatcatg gcagcgatga catagggttt gtagctggct tctttataga
     4081 gagaatcgcg atagcggtca aagacagagg cttcgacttc gccgtcttca cagctgacgc
     4141 cgttgatgtc ggccggcagg cagtggaggt acatggcttt gccgtctttc gtggtcttca
     4201 tcaattcttc tgtacagcac cagtctttgt gctgggcgtt ctgggctaag agttcctgtt
     4261 ccagggcgtc gataccgtcc tggtcgccag cggcatagag gttggtccgc ttttccatgg
     4321 ctgcaaaggg agcccagctc ttgggataga cgacgtcggc gtctttgaag gcgtcggcca
     4381 tgtcatgggt gatgcggaag gaaccgcccg atttttcagc gttctttttg gcgacgtcga
     4441 tgacgtccgg catgacttca tagccttcgg gatgagccag gacgacgtcc atgcccagac
     4501 gggtcatgag gccgatgacg ccctgcggga ccgagagggg cttgccatag gacggggagt
     4561 aagcccaggt catggcgatt tttttgcctt tcaggttttc caggccgccc atttcgtgga
     4621 tgatgtgggc cgcgtcggcc atacactgag tcggatggtc gatgtcgcac tggaggttga
     4681 ccagcgtcgg tttctgttcg aggatgccgt ctttatagcc ctgcgtgacg gagtcgacga
     4741 cttctttctg gtaggcgtgg cccttgccga tgtacatgtc gtcgcggatg ccgatgacgt
     4801 cggccatgaa ggaaatcatg ttggccgttt cccggaccgt ttcgccgtgg gcaatctggc
     4861 tcttcttttc atccaggtcc tggacctgca ggcctaagag gttgcaggcc gaggcgaagg
     4921 aaaaacgggt ccgcgtcgaa ttatcgcgga agatggaaat gcctaagccg ctttcaaaaa
     4981 tctttgtgga aatattgtgt tcacgcatga agcggagggc atcggccagg acccagatgg
     5041 cttccagttc gtcatcggtc ttgtcccagg tgaggaaaaa gtcgttttca tacatatctt
     5101 tgaagttgag ggcctttaat ttatcgatgt agtcctgtaa ggttttcata gggcagtctc
     5161 cttttaatcg ttatgggatg tgattattta tcggtgtata cagaaggtaa tgcggcgtag
     5221 acggcggcac aggtgacgag gtcctgtttc catgtctttt cgttcggtgc atgggcctgg
     5281 gcttctgcgc cggggccaaa gccgatgacg gggatgccgt tgcggcccat gatggaaacg
     5341 ccattggtcg agaaggtcca tttatccgtc aaggggcggg ctttgcgcat ggctgccgtt
     5401 tcggcagtgc cgatgcggct gtcgccgaag agggaggtat aggcttcttc cagggctttc
     5461 gtgactttgt ggtctttcgg aatgacccac gtcgggaagt aacattcgat gggataggtc
     5521 aggccggtgt agctcggacg ggcgtattca tacatgctga ctttgacatc atcgccgtac
     5581 ttctggacgc tcggcagggc acggatttca tcgaggcagc tctgccacgt ttcgccggcc
     5641 gtcatgcggc ggtccaggga gacggcacag gaatcggcga cggcgcagcg gctcggcgag
     5701 gtgtagaaaa tctgggagca cgtgaccgta ccgcggccga ggaagttggc ttcttcccat
     5761 tcgggattgt atttcttgtc gagcatcttg accagacctt tgatttccgt cgtgtcggct
     5821 gcgtcatttt cgttgagggc ccgcacgtcc tggaggatgt cggccatctt gtaaatggcg
     5881 ttgtcaccgc gttcgggcgc gctgccgtgg caggatacac cctggacgtc gatgcggatt
     5941 tccatacggc cgcgctggcc gcggtagatg ccgccgtccg tcggttctgt ggagacgacg
     6001 aattccgggc ggaccttgtc ttcgttgatg atatactgcc agcagaggcc gtcgcagtct
     6061 tcttcctgga ccgtgccggt gaccaggacg gtgtacttgt cggacaggag gcccaggtcc
     6121 ttcatgatgc gggcgccgta gacggcagag acgatgccgc cgagctggtc cgacgtgccg
     6181 cggccgccga tttccgtttc cgattcaaag ccttcgtagg gatcgaaatc ccagttggcc
     6241 ttgttgccga tgccgacggt gtcgatgtgg gcgtcgaagc cgatgagggt cttgccggtg
     6301 cccatgtagc ccaggacgtt gcccatgggg tcgatttcca ctttatcgaa atcgagtttg
     6361 cgcatttctt cggcaatgcg gtcgatatgt tccttttcgc cgcagctttc accggggaat
     6421 ttaacgatgt cccgcaggaa acgggtcata tcctgttcat agcctttggc agcttctttg
     6481 attttttcgt acggtactgt catgaaaatc actcctatat cttatatata atgttttact
     6541 tattcaaacc gcgccagacg atattcttat agcgttccgg gtccgtgtcg ccttctgtcg
     6601 agaagaccag gacctgtgaa tggtcgtcga ggccgagttc agcgcgcagg tccttacatt
     6661 catcggataa catgatcgta gccagggtgc cgaagggaac ggcaccggat tcaccggata
     6721 cgatgggcgc atcgcctttg aggggcgctg ccagcatacg cataccgcgg accgtgatgg
     6781 ggtcatcggc tgcgatgaag gtcgtcacat ggttcttgag gatgtcccag gaaatggtgt
     6841 tcggttcgcc acaggccagg ccggccatga tggtatccag gtcgccgccg accatgcggg
     6901 gcttgccgtc accggctgcg gcgcctttgt agaggcaggc tgcggccgat gcttcgacga
     6961 cgatgaattt cggcggattg tctttataga ggttggcgaa ataaccgacg acggcaccgg
     7021 ccagggagcc gacaccggcc tggacgaaga tgtgcgtcgg ccgttcgatg ccctgggctt
     7081 tcagctgttc gccggcttcc atggccatcg tgccgtagcc ctgcatgatc cacgacggga
     7141 tttcttcata gccgtcccag gccgtgtcct ggaccatgac gccgtgcggc gtagcggtgg
     7201 cttctttggc tgcctggcgg acgcagtcgt catagttgag gtcgaggatg tcgacgtcag
     7261 cgccgagctt cttgatgttt tccagccgcg tctgggtcgt gcctttcggc atgtggacga
     7321 cagctttctg gcccagcttc tgggcggccc aggcgacgcc gcggccgtgg ttgccgtcgg
     7381 ttgccgtgaa gaaggttgcc tggccgaatt cctgacggag ctcatctgac gtcaggacgt
     7441 cataaggcat gtccgataca tcgcggtgcg tttccttggc gatgtacttg gccatggaat
     7501 aggaaccgcc caggactttg aaggcattga ggccgaagcg gtacgattcg tctttgatga
     7561 aaaccgagcc gacgcccagg tagcgggcca tctgcgtgag ccgggtcagc ggcgtttcgc
     7621 tgtattgcgg gaaactctgg tggaatgtcc gggccttggc gacttcgctg agggacatga
     7681 tcggcaaatt tttatcatct gatttgggca gatggttcat agcccatttc atatctttct
     7741 tcatatccgt acctcctaaa gataaactga aaaaaatatt ttagcaaatc taaaagtttt
     7801 tgtataccta gagtataaca tgaccttaca gatttgcaat agaaaagagc taaaaatttg
     7861 ttaggtatct acactttttt tgcaatgaca gaaagagaac tgttacattt gggcatagga
     7921 aaaggcgctg tcgcataaag acagcgcctt ttaagatgat tcaggcgggc cgccctttgg
     7981 gcggccccta ctatgatgcg ggcacgagcc acgaaccacg aaccacgagc cactaatctc
     8041 actcttctcc gctgtcgatg tagctgtaca gggtatattt ggagatgccg aagtgtttgg
     8101 agattttgtc gccggccttg gtgatcagca gggccccgcg gtcgttgagg aatttgatgg
     8161 ctttgatctt gtcttcccgt gtcatgaggg ggacgggttt gccgacctgg cgcgtcgatt
     8221 cgtcgatgag gtcgttgagg aggtccgtga cattctgcgg gatacggctg accttatctt
     8281 ccttggacgt gaccgagatg agtggcttga tggcattttc gaccatcatc agttcagtga
     8341 tgtcgtagtt gatggaaaag atgccgatga ttttttgctt ttcatttcgg atgtagatgg
     8401 tcgtcgactt gaggatgcgg ccgtctttgg tcttggtcag gtaactcgta tggtcctgga
     8461 gcttgtcggg atcgctgcgc agggcgtcga ggacggccat agacgggcca tcgcctactt
     8521 tgcggttggt gacgtggcca ttttcgatgg cgacgatgga attttccttg taattatcgc
     8581 tcaagtcatg gatgacgatt tcacagttcg ggccgaattg gtctgccaga cctttggcta
     8641 agtgacgcaa aaagttgagg acgacgtcgt ctaacataag gggcacctct ttctcatatc
     8701 aaaaacagat tattagctta tctgctaaaa attttttagg tactgttact atagcatatt
     8761 ttcgggataa atcaacggcc cttggtgaaa aaaggtatgt tgaaaaaaca tctttcctgt
     8821 cgagctataa caaaaagata ataaaaaatt tttagactaa aaaaaaattg acaggtaagg
     8881 cggaacgggt tactattaag atagatacga gaatgacaca aataacatgc attaactgca
     8941 agaaacctta taatttatga tttgtatagc atagaggagg tatacttatg ttaatcgtcg
     9001 gaaatggtca cgttgttacc cgcgacgcga agaatcaata tttccctgat ggcgccgtag
     9061 ccatagacgg tacgacaatc tgccgcgtcg gccagacggc agaaatccgc cgggcctatc
     9121 cggacgccgc ttttatcgat gccaaaggcg gtgtcatcat gccggctttc atcaacgtcc
     9181 atgagcatat ttacagcgcc atggccagag gcatgaatat cgacggctat gcgcccaagg
     9241 gcttcctgga catcctcgac ggcctgtggt ggaacatcga ccgtcacttg accaacgacc
     9301 tgacttattt gagcgctatg gaaacgtatg tcgaatgtat caagaacggc gtgacgacgg
     9361 tcttcgacca tcacgccagc tttggcgaaa tcaaggacag cctctttgcc atcggccagg
     9421 cggcgcagga actgggcgtc cgctcgtgct tgtgctatga agtctccgac cgcgacggtc
     9481 aggacaaagc ccgccaggca gtcctggaga atgaagcctg gatacgccac gctcaggccg
     9541 acacgacgga catgctcgcc ggcatgatgg gcatgcatgc ccaatttacc atcagcgacg
     9601 aaacgatggc cctggcagct gaacataaac cggacggcgt gggttaccac atccacgtcg
     9661 ccgaaggcat cgaagacttg caggattgcc tgaagaaaca cggtaaacgc attatcgacc
     9721 gcctttatga ctggaatatc ttagggccca agaccctgct cggccactgc atccacgtca
     9781 atccccacga aatggacctc atcaaggcga cggatacgat ggtcgtccac aatccggaat
     9841 cgaacatggg caatgcctgt ggctgcccgc cgacgatgga catggtccac cgcggcatcc
     9901 tgaccggctt ggggaccgat ggctatacgc acgatatgat tgagtcctat aaagtcgcca
     9961 atatcttgca caaacaccat ctctgcgacg ctacagccgc ctgggctgaa gtaccgcaga
    10021 tgctgttcta taacaatgca gccatggcca accgctattt caagacgccc ttaggcgtac
    10081 tggcagaagg ggctgcagcc gacgtcatcg tcatggatta catcgctccg acgccgatga
    10141 atgaagaaaa catcaacagt cacatcttat tcggcatgag cggccatgat gtcgtcacga
    10201 ccattgccaa cggcaaagtc ctcatggaaa accgccggct caccggtgtc gatgaagaag
    10261 ccctgatggc caagatccgc gaaggcgcag cgaccctggc cggacgcatt aatggatact
    10321 tataaggagg aatctatcat gagtgatcgc atgacatcca ttccctttgg aaaattgatg
    10381 aactgggtcc tgactgaagc gccgtctggg acaatctttg gcgtccacca tcgcctacag
    10441 gccgacccaa atcggacata tactatcttt gacagaccgc tggaaatgcc ctttggtccg
    10501 gctgccggtc cccataccca gctggcccag aatatcgccg ccggctatgc ggcgggagcg
    10561 cgcttctttg aactgaagac ggtccagaaa atcgacggcg aagacctgcc cgttcccaaa
    10621 ccctgcatca aggccgacga tgaatgttac aatgtcgaat ggtcgacgga actgaccgtc
    10681 ccccaggccc tggaagaata cgtcaaggcc tggttcctgg tccacgtcat ggccgtcgaa
    10741 tacggcttag ggagtccgga cgggatgcag ttcaacatgt ctgtcggtta cgacctggca
    10801 gggatacaga cggcgaaaat cgattcgttc atcgaaaatc tgaaagacgc atcccatacg
    10861 gccatctttg ccgagtgcaa agcctggctc ctggcccatc tggaccggtt ccagaaactc
    10921 cgccgggaag acgtcgaagc cattccggta gaaatctgca actctgtcac ggtctcgacc
    10981 atgcacggct gtccgcccga tgaaatcgaa cggattgccc gctatttact gaaagaaaaa
    11041 cacctcaata ccttcgtcaa atgcaacccg accttactgg gctataactt cgtccggaaa
    11101 tgcatggatg acctgggcta tggccatatg gtcttcgatg acagccattt caaggctgat
    11161 ttacagtacc gtgatgccgt acccatgctc aagcggctgc aggaagcggg taaagaggaa
    11221 ggcctgttgt tcggcgtcaa actgaccaat accttccccg tcgacattac gcgccatgaa
    11281 ttgccgggga cggaaatgta tatgtccggc aagcccctct tcttcctgtc catgaatgtg
    11341 gccaagaagc tgagtgaagc ctttgacggc agcctgcgta tttcttacag cggcggtgcc
    11401 gatgcccaca acatcaagga tatagtccgg gccggcatct ggccggtcac ggtggcgacg
    11461 acgctgctca agcccggcgg ctatgaacgc tttgcccagc tggcacagtg ttttgaaggt
    11521 cagcgtggac aggcgtttac tcgtgtaaac gtcaaggccc tggatgactt gctggctaag
    11581 gctcaggatg atgcccacta tgcccgtctg gacccgaagg cccagcagcg caagaacgac
    11641 caggcactgc ccttgatgga ctgcttcatg gcaccgtgta gccagacttg ccccatccat
    11701 caggatatcc cggcctatat ggacctcgtc gccgccggga aatatgacca ggccctgcag
    11761 atcattctgg aaaagaatcc cctgcccttt acgacgggga ccgtctgcta ccatacctgt
    11821 atgaacagct gtacgcgcaa tttctgcgat gcccctgttg aaatccggcg caataagctg
    11881 atagctgctg aaaaaggcta tgccagcgtc atgacgactt tgcaggctgc gccgtccaat
    11941 gggagaaaag cggctgtcat cggcgccggt ccggccggac tgtcggcggc ttatttcctg
    12001 gcccgcggcg gcctggatgt gaccgtcttc gataagaacg acgaacctgg cggcctggtg
    12061 cggaccttct tgtgtgaaaa gaaagggcag atttcaccag aagccatcga caaggatgta
    12121 gccctcatcg aaaagatggg tgtccatctc gtcgtcggca gggccatcgc ctcgctcgac
    12181 gaattgcagg gctatgacat catcctggcg gcctgcggcg tcaaagggaa aatcggtgac
    12241 accctggccg gcgccatcga aggcctgacc gtcatcggcg atggccatta cggcaagacg
    12301 acatccgtcg tcgaatgtat tgccgacggc aagcgggcgg cggaaggcat tttaggccag
    12361 atggccagtg tcgataccct gattccagct gatgtaaacg acgtttactc gaagcggggt
    12421 attctggaga cggcaccgga aacaggctgt gacggccgct gcctccattg tgacagcgtc
    12481 tgcgaagtct gtacggaagt ctgccccaat cgggccaata cagccatcca ggtaccgggc
    12541 catatgcagg ctcagattct gcatattgac tacatgtgta acgaatgtgg caactgccgg
    12601 accttctgtc cctggggcgg tgcgccgtat gtagataagt ttacactctt cgctaatgaa
    12661 gacgatttag accacagcga taattcgggc tttgccgtcc tcgacaaggc cagcggcttc
    12721 tgcaaagtcc gcctggatgg agaaatccgg acgacgactt tagggacggc tgatgaagca
    12781 atcccggaag acctgcggac cttcatggaa acggtctgcc gcgattacga ttacctgctg
    12841 attgaataga gaaagaaggg aaaaccatgg attacgtcat caaaaacggg acggtctgcc
    12901 tggaagggga acatttccgg gccgacgtta ccgtccgtga gggaaaaatc caggccttgg
    12961 gacaggctgt gcacagcggt catgaacagg tcatcgacgc cgacggcctc tatgtctgcc
    13021 ctggcggcat cgatgcccac acccatatgg acctgcagca gtcgccgcag taccgcgcgt
    13081 gcgatgactt ttacagcggt ggcgtggcag cggcctgcgg cggcacgacg accatcctgg
    13141 atcatatggc ctttggcccg gccggctgtt ccctgcgcta tccctttgaa cggtaccgga
    13201 aactggccgt gccctgcccg gtcgactaca gcttccatgg cgtcatccag catgtcgacg
    13261 accaggtcct gcaggaattg ggccagctca tcgacgaagg ttttcccagt ttcaaagcct
    13321 atacgacgta tggctatccc gtcggggaga agaaactgat gcagctcctg ccggtcatca
    13381 agaagcatgg cggcctgctc acggtccatg ccgaaagcga cggcattacc aacgccctgc
    13441 gggatgccct gggaccgcag gacctgcagc ccatcggcca ggcgaagacg cggcccaacg
    13501 tggccgaagc cgcagccgtg agccgtgtcc tggcggcagc ccgggcttgt ggcgatgcgc
    13561 cggtctatat cgtccacacg tcggcccatg agagcctgga ccagctgcgc ctggcccggc
    13621 gccaaggcca gcgtcatatt tacagtgaaa cgtgtccgca atacttattg ctgacagaag
    13681 agaaattccg tcacggcggc cctatggaag gcattaaata catgctggcg ccgccgctgc
    13741 ggaaagccga agacaaccag gccctgtggc aggccctggc cgacgggacg atccagaccg
    13801 tagcgacgga ccactgcccc tttacgatag ctgaaaaaca ggagaatgtc agcgatttcc
    13861 ggaactgtcc cggcggcgta tccggcgtcg aagaacggat gcccctgctg ttcagtgaag
    13921 gcgtcctgaa aggccggctg accctggagc gcttcgtcca ggtgaccagt gccaatgtgg
    13981 ccaaaatctt tggcctctat ccgcgcaaag ggacgctgct gcccggcagc gatgccgaca
    14041 tcgtcctcat cgatccgaaa gggacgcgga cctttgccag ggagaacctg cacacgacct
    14101 gcggctattc gccctatgaa ggcatgacgg tccaggcgac gatccgcctg accatgctcc
    14161 gcggcgaaat catcgccaaa gacaatcaat tctgtggaaa acgcggctat ggaaccttgt
    14221 tgtatcgatt ccgcgataaa agataatctt gattatagga aggtggcatt atgagtgaat
    14281 tatgtaaact atgggtaaac ggccgtgaaa tagaagccga tggagaacgc aagctcatcg
    14341 acgtcctgcg caatgacctg cgcctgaaat ccgtcaagga tggctgcagt gaaggggcct
    14401 gcggaacctg tacggtcgtc atcgacggca aggcgaccaa gacatgtgtc caaaaggtca
    14461 gccgcatggc tggtaaacac gtgttgaccg tcgaagggct gtccaagcgg gaaaaagacg
    14521 tcttcgtcta cgcctttggc gaagccggcg ccgtccagtg cggcttctgc atccccggca
    14581 tggtcatgtg cggcaaggcc ctcatcgacg ccaacccggc gccgtcgcgg ctggaaatcg
    14641 ccgcggccat ccgcaataat atctgccgtt gtacgggtta taagaaaatc atcgacgcca
    14701 tcgctatggc agcagatttg ctgcgcacgg accagcctct tcccgtccgc ggcggctacc
    14761 gcggcgtcgg cgctccctgc cagcgcgtca acgtcaaggc caaagtcctg ggcgtcggca
    14821 gatatacaga tgatttggac gccgtcgacc tgcccggcat gtgttacggc agtgccatcc
    14881 gcagcgctta tccgcgggct gtcgtcaagg ccatccatgc ggaaaaggcc aaggccctgc
    14941 ccggtgtcgt ctgcgtcctg acggcagacg atatccccgg gtccgtcaaa gtcggccatt
    15001 tgaagaccga ttgggatacg ctcatccccg tcggctcgac gacgcacttc ctgggcgacg
    15061 ccatcgccct ggtggctgcg gaaacgccgg aaatcctgga acaggctaag aagctcgtcg
    15121 atatcgaata tgaagaactg ccggccctga cctgtgcagc cgacgccctg aaagaagggg
    15181 cgccgctggt acacctgtcg gctggtacga acgtcctggc ccatgaacac ctggtccgcg
    15241 gcgacgctga tgccgtcatt gccgcctcga agtataaggt gacgcaccac tttgaaacgc
    15301 catggacgga acacgccttc ctggaaccgg aatgtgccgt cgccatgccc tttgatgacg
    15361 gcgtcttcgt ttattcgtcg gaccagggca tttatgatac gcgccgggaa acggcactca
    15421 tgctgggcct gccggaagaa aaagtcatcg tcgagaactg cttcgtcggc ggcggcttcg
    15481 gcggccggga agacgtgacg gtccagcatt tggctgccct cgtcgcctat gtatgtaaac
    15541 ggatatgtaa agtgaagttg acgcgccagg aaagcatcct catccatccc aagcgccatc
    15601 agttcagcat ggacatgacg acggcctgcg atgaaactgg gaaactgacg gctatgaagc
    15661 tcgtcgccgt caccgacaac ggggcctatg cctccctggg cggacccgtt ctccagcgcg
    15721 cctgtaccca tgcggcaggg ccttataact accagaccat cgacatcgac gggacagccg
    15781 tttacactaa caatccacca gccggagcct tccgcggatt tggcgtcact cagaccattt
    15841 ttgccacgga attaaactta aataaactgg ctgaactggt cggcatcgac ccgtgggaaa
    15901 tccgatatcg caatgccatc cggcccggtg acatcctgcc caatggccag attgccgacc
    15961 cgtcgacagg cttggccgaa acgctggatg ccgtcaaaga ggcgtactat cacaacgggc
    16021 cttatgtcgg catcgcctgt gccttgaaga actccggcgt cggcgtcggc ctgcccgact
    16081 atggccgctg ccgtctggtc gttaaagacg acagggtaca catttactcg tcggcatcct
    16141 gcatcggcca gggcgtcggc acggtcctgg tccagatggt atcccaggca gcaggcctcc
    16201 ccattgaaga tatcaaatgg cacatgccca atacggcgac ctcacccgat gccggcacga
    16261 cgtcgggttc gcggcagacg acgttgaccg gggaagccgc ccggcgggcc gccgtccagc
    16321 tgcggcgcga cctctttgcc gccaagggcc tgccttacga agaaacgagc catgccagcg
    16381 actacctgga aggcgtaacg gtagaaaaag aatcgcccgc tacggcagcg gtccgcttca
    16441 accgcagcga cttggatatc ctcaacggcc gggaatacat cggcgaatac ctgggcaaga
    16501 ccgacaagat gggtgctgac atcccccatc ccgtatccca tgtcgcctac ggctatgcca
    16561 ctcacgtctg catccttaac gacgacggta ccatccgcaa aatggtcgcc gctcacgacg
    16621 tcggccgcgt cgtcaaccgc aagagcctgg agggccagat cgaaggcggc accgtcatgg
    16681 gctgcggtta tgctctgaca gaacaatatc cgttggacca ctgtaaaccg acggccaaat
    16741 tcggaaccct gggcctgttc cgggccgata aagccccgga cgtcgaagcc attcccatcg
    16801 aaaaagaagg cgtcgacgta gcctacggag ccatcggcgt cggcgaaatc acatccatcc
    16861 cgacggcacc tgccgtagcc ggagcctact ataaatggaa cggccagttc cagacaacat
    16921 tgcccttaga agggacgccg tatagccgga agaaaaagaa ataaaagttt gtagtacaga
    16981 gtttgtggtc tgtagaagag ggccttaaat ttaaagccgt ctgctagcca ctaacaacta
    17041 aagagtgctt gacgtcttgc gacaagcact cttataatag ggatgtttga tgtttcgatg
    17101 tggaaaggtg gtcttatgga agataaattg gtcattatcc gcggggctgg ggacctggcg
    17161 acgggcgtgg cccagtcttt gtatgaggct gggttccgcc tgctgctgct ggaagtggag
    17221 aagccgtcgg ctatccggcg tcaggtggcc ctctgtgaag ccgtctacga tgggtcggct
    17281 gccgtcgaga acctgacttg ctgccgctgt cagtctttgg ccgaggtccg gaaggcctgg
    17341 gaaggcgagg ccattccctt gctggtggac ccggaagggg cgtcgattgc ggcgctgaag
    17401 ccctgggccg tcgtcgatgc catcctggcc aagaagaact tggggacgaa ccggcgtatg
    17461 gcgcgtcata ccgtggccct gggaccggga tttacggccg gtgtcgatgt cgatgccgtc
    17521 attgaaacca agcggggcca ccagctgggg cgtatcattt ggcagggcac ggctattgcc
    17581 aatacgggga ttcccggatt gattgccggc tacggcaagg agcgcgtcat ccacagtcag
    17641 cagtccggat tcgtatacgg attggtccac atcggcgata aagtaaaaaa aggacagccc
    17701 atggctgtcc tgacagaaga gcccatccct tttggaatgg ccgtgacgga atgtatgggg
    17761 acgccgattc tggcgtcgct gacgggattc gtccggggac tgatccgtga cggctatcag
    17821 gtaccgcaag gcttcaagat agccgacatc gacccgcggg atgatgaacg ggagaattgc
    17881 tttacgattt ccgacaaggc ccgcagcctc ggcggcgccg tcctgacggc cctgctctgg
    17941 atggggaagg gggaattttc gtgaagatga atcagaatga cttacatttt tcgtcgtgcc
    18001 gggagctgcc ctatatcgac tattaccaag gcgcagcagc taagaagcag tccattgccg
    18061 tcgaagtgat gacgccgctc tatctcgatg ggcagcgcat cagtacgctc atttcttcgc
    18121 cggaagatat cgatgtcctg gctgtgggtt actgtctggc cgaagagaaa ataccggctg
    18181 gccgcaagct ggctggtgtc tgcctgcacg atggggcggc ctgggtcacg acggaaaccg
    18241 ggacggcacg ggcagtagaa aagccgcctt ttgtgccgat tacggcagag aagctctgtt
    18301 cctatggagg tctcttggac agcttatcga cggcacatca cgcctcgcac ggcgtccacg
    18361 aaggggcctt ggtccaaggc gatgaagtcc tcgtctatac ggaagatatc ggccgccaca
    18421 atgtcctcga ccgcctgcgg ggcctcgtcg aattacagca catcgacgtc agtcaggccg
    18481 tcctggtctt cagcggccgc gttccccaat cggtcatcaa aaagatccat ggcctgggcg
    18541 ttcgcttcct ggcatcgcgg gccttgccga cgagtctggg cgtgtccctg gcggagcagt
    18601 accagattac cctggcctgc ggcttgcggc ctgattcttt caaaatattt gctcatccag
    18661 aacggatttt attataatat aaaaaagtgt cacatgaagg cattttggct tcgtgtgaca
    18721 gcttttttat gtagggctcg cacgtggcga gcccgcttcg ggatatgtat gatttcccga
    18781 agtggttgct aaaaaatcgt cccaatttct aacgggtcgc ccacgtggcg acccctacag
    18841 tgcaaacaag ggcttgtcgc tgtgcgacaa gcccttttta taggaatatt acttcaatgg
    18901 gagcgccctt tggcaacggg ggcgttcctt tttttatata cgccagggcg ttggtgtggg
    18961 caaggcccag cagggcactg gacgtcagtg tcgtattggg tgtgaatatg ggctggccct
    19021 gttcgtaggt caggcggccc tggacgaagc ggtcgacagg atttttctta tagatcgcgt
    19081 cgcctaaatg gcaggtgacg acgggaaatt cgctgtttct ttcgccgttg aggcggcgca
    19141 ggatggggac ggccaggaga tggaaggcat tgaacgacgc cgaaggattg ccggacaggc
    19201 cgaggatgag cgtccggcgg ccgtctgttt caatgacgcc gccataggaa gccgaaccgg
    19261 gccgcatagt gatgcggtcg taaagttgga cggcaccgag ggattggtaa atctggggca
    19321 tcgtatcata gaggccgacg gagacgccgc ccgtgctgac gatgaggtcc ttgtcccggg
    19381 ccaggcggcg gacggctgcc atttcatcag ccaactggtc cggtccgtcc gatacgtgat
    19441 agaaggtgac ttctgcgccg tactgttcca ataagggctg gaagagatag gcgttggagt
    19501 tgtaaatctg gccatggtgc cgcacttctc cgggcaggac cagttcccgt cctgacgtca
    19561 ggaagaggac cttcgctttg gcgtaaacgg gaaggcgttc atttcccagg ccgacggcga
    19621 cggacagggc accggctgtg agcttcgtcc cggccgggat gatgacggcg ccggaacggc
    19681 actcttcgcc tttggggatg atgttgctgc ccggcgggat gctttctgta actcgtatat
    19741 gatttccctg gcgttcgacg tattcctggg ggacgacggt atcgcaatca tccgggacgg
    19801 cgcagcccgt catgaggcgt acggcctggc cggctgcaac agggccgtca tagacgatgc
    19861 cggcgccgat ggtcgccacg acttcatagg catttccgtc atggctatgg atagcatagc
    19921 cgtcgaaggg cgatttacgg tacggcggat agtcgcaggc tgccgtaatg tcccgggctg
    19981 tgatgcggcc cagggcctgg tttggtgtga catattctat tttcgcttca taggaaggca
    20041 aggcgccgtc gagacggcgc cttgcttcgg taattgtgac gatttccata gttatggctt
    20101 tccttttttc aatgcttttt cataagctgc ctgtgttttg gccgcttctt tttgggcttt
    20161 cagtttagcc cgctgagccg cgacgacggc cgggtcgaag agactcaggg cttccgttgg
    20221 gcaggccggg atacaggctg gttcccccgt ttccgtattg gcacagaggt cgcatttgta
    20281 ggcgacgatg cggaagggcc ggcctttggt atcgccgtgg tagatcttgg tcatgttgat
    20341 ggcctggaaa ggacaggcgc gggaacattt tccgcagccc cggcaggcct cttcatcgat
    20401 gacgaccgag tcgggaccca gggaaatgca gtggaacgga caggcctcca tacagcgcgg
    20461 cttcgggcac tgctggcatt ggacggcctt ggtgactttc gacgtgtaaa cgacgtggac
    20521 ccgcggcgtg aaatcgaagc tcttgggctt gagggcaaac agggccttgc ctgtatggct
    20581 ggccacacag gcggccatgc aggtcttgca gccgatgcac agttccgggt ccgtcttgac
    20641 gtgtttaaac atcgtttaca cctcctttac ttcgatgccc atgcggctgc ggatagcggt
    20701 atactgggct tttacatcgc gttcggcctg tttctggtcg tcgatttttt cgacgcggca
    20761 ggcacagtat ttgaattccg gcgtatggct cttggggtcc agggcggccg acgtcaattc
    20821 gttgcaggcg ccgacccacc actgataggt catgtacgtc gcccctttgg atacgcggtc
    20881 tgtttcctgg cagcgggtga tggtatggcc ccgcttggag acgacgcgga ccaggtcgcc
    20941 gttggcaatg cccagttcct ggcagtcttc gggattcatc tggatatagc ccggttcatc
    21001 ggccaggtcg gccaggaggc ggcagttgcc ggtcatggta cggaccgaat agtggccgac
    21061 ttcgcggact gtcgacaggg acagggggta ttcgggactt tccagctctt tggccggcgt
    21121 ccaggggtag tagtagaaat ggcctttgcc gtcggccgta gcaaaatgag cgcccttgtg
    21181 gagatactgt gtccccgtat catccgggct gtcttccgtg cagggccatt ggacgccgtg
    21241 gttctcgacc atcttttcat agctggcacc ggcgaatttc gggcacaggg agcgcatttc
    21301 gtcccaaatc tgttcggtat tgtcgtaatg catgggatag cccatggccg ttgccatgag
    21361 gcagaggatt tcccagtccg tcttgcagcc cggctggggt tcgatgagct tgcggacgcg
    21421 ctggaagcgg cggtcgcagg aggtatatac ggcattgtgt tcgccccagg ccgtagccgg
    21481 gaagatgaca tcggcatatt cgcaggtctt gttgaagaag atgtcctgga cgatgaccag
    21541 gtcgatagcc gacagggtgc ggcggatttc cggcaggtcc ggatcggact gggccgggtc
    21601 ttcgccgatg atgtaatagg cgtgaatccg cttcttcggg tccggttcgc gcaggacttt
    21661 gtccggcaca taggtcaggg gcaggccgac gtgggatggc agggaaacgc cccaggcctt
    21721 ttcaaattta tggcggattt catcgtccat gacgtcctga tagccaggat actggttggg
    21781 caggacgccc atgtcacagg tcccctggac gttgttctgg ccgcggaccg ggccgacgcc
    21841 ggtggcgtaa tggccgtagt tgccggtcat gagactcagg ttggagcagg cgatgacgca
    21901 ttcgacgccc tgtgagaact gggtgatgcc catgccccag agggtcatgg tatgtttgga
    21961 cgaagcgaac aggcgcgctg cccggcggat atcgtcggga tcgaggtggg tcgctgcggc
    22021 gacggcttcg ggcgcgtatt tatcctgggc cacttctttg gcatagtcgg caaagcctgt
    22081 cgtatgcttt tcgatgaaat cgtggtcgac caggtcctcc gtgaccatga cgtgggccat
    22141 ggcgttgagg aagagcatgt tcgtgccgcc cttgataggc aggtaaatgg tggcgatgcg
    22201 gcccgtttct gtcaggcgcg gatcgacaca gatgatcttg cagcccttct tcttggcccg
    22261 gacgatatgg cgggcgacga ggggatgcga gaccggcgcg ttataaccga tatcgaagat
    22321 gacttctgcg tcttcgagtt cgggaacgct gaaagacatg gcaccttcac cgacggactc
    22381 accgagtccg gtaaccgatg gagcgtgtca aatgcgggcg cagtggtcga cgttgttcgt
    22441 gccgacgacg gcgcgcatga atttttgcgt aatgtaagcg gcctcattgc cgggaccgcg
    22501 ggccgaaccg gcgcccatga cggcatccgg gccgtattcc ttgatgatgg ctttgaggcg
    22561 ggccgctgcg tattcgatgg cttcctgcca cgatacttcg acgaggggcg tgcccttgcc
    22621 gtttttgcgg atcatcggct ggcggatacg cttggtcagg atctgagggt cattcagata
    22681 gtcccagccg tacagccctt tcagacagag ccggccttca ttgttgacac cgcgcggcga
    22741 gaccttggct tcgatgacct ggtttttttc cgtgtcgacc gtgcaggtga tgacgcagcc
    22801 tgtgccacaa tagggacaga ccgtgtcgat tttcttatag gcacacatag gaacacctca
    22861 cttttacata aactggagat ttttagtata ccacggacgg ggtacgcttt actagtctca
    22921 gtgtaggact tattttagca gggtgcaaga cagaaaagaa caaactgctt atatcaccga
    22981 cgagcggtag ccagtgagga ataagatatt gttgaccagc tggttgtcat cggcctgggt
    23041 cacgtcgatt tgtggaaagg gaccgtgact gcggctttcg acgaggacca ggtcggccag
    23101 ctgttgctgg gacagggcca ggatgtaatc ttctttccgc gttcccatgg gataatctgc
    23161 cggaccgcag agacagacgg aaacggcacc ggctttgcgg gcccggtccg tatcggagcc
    23221 ggtcttgcgg cgggccacgg tgtgatggct gctcttgata taggcgacct gatagccgcg
    23281 gtgcgtcagt tccgtaatgg ctgccgcagc ggctgtcgtc ttgccatcgc ctgatttcgg
    23341 tgccgtcagg gtgacgacgg gtacctggcg gcggctgttg gtgtcgcggc ctttggcagc
    23401 ctcataggct tcaggcgtat tgatattgaa atataaggcc gaacggaagg tttcatcgac
    23461 ataggctgcc ggcacgtctt gatacagatg gcgcaggcgg taattgccct tctgcagatg
    23521 gtccgtaatg gctggcagga gctgacggct gtataaggcg gccaggggct ggtcctggcc
    23581 gttttccgtc ctgggtatga tggcctgcag ggtgggctgg cgccggagta atttgaccaa
    23641 atccggggcg ggggcaaaat catagaaggg aaggtcgaca gataagacca gatagaagtc
    23701 gcactggccg ctgtacaggg cggcctccat cccgcccaag gggccgcagt caggataatt
    23761 gtctatgacc aggcggactg tgcgggctaa tttggcgggt aaatgggacg attcataagg
    23821 acggttgatg gaaagcaagc gctctgtaaa gggataagct tggctgcgca gaagcagatc
    23881 cgtcagcagg tccgtatcgc gccaggggag ggccgtttta tcgcggccca tgcgggtact
    23941 gcggccgccg gccaggataa tcagccccag cgataaaggg cgaagtattt ttgtcatcct
    24001 atcacatcct tttgtatgtt atgcgtgtat tatatcataa tttgcataag cggtgatgat
    24061 ttcactacgg gttacaaaaa aagtatggcc agctaacaga ttttcatgta taatgagctt
    24121 agtagattcg ttatttgtaa gcgcgaataa cgcttgaagg aggtggctcc atggcagaag
    24181 agaaactaca ttgcaaagtg accctgtgcc tcatgggcga taagcgggcc ttcgggcccg
    24241 gcgtggccga actgctggaa ggtgtccgcc gtcatggttc cctgcagggc gcagcccggg
    24301 atatgggcat gtcgtactcc aaggcctgga cagtcatgcg caacgccgaa gaaatctggg
    24361 gctttcccct catccgccgc tatgtcggcg gcaaagacgg cggccggtcc gtcctgacca
    24421 agcaggctga aatcatcctg gaacggtatg aagagatgaa cgaggccctg cacgacgtgg
    24481 cagaccgcca gttccggcaa tggttcaatg atgaagaact ccataaatta caaaaaatgg
    24541 aaggatagat aagtatgaaa atgatgaaaa ccgaagacgc tgtcggccat gtcctctgcc
    24601 atgatattac gcagatcatc cgcggcgtca ccaaaggccc ggtcttccgc aagggccatg
    24661 tcattaccca agaagatatt ccggtcctct tgtcggtcgg caaggaccac gtctatattt
    24721 gggaaaaaga tgacaccctg ttccatgaag atgaagccgc tgccatcctg cgcgacatct
    24781 gcatgggagc tcatatgcac ggcagtgcgc cgaaagaagg gaaaatcgaa ctcatcgccg
    24841 actgcgacgg cgtcctgact atccgccggg atggcttgaa agccgtcaat tccctgggcg
    24901 aaatgatgat tgccacccgc cacggcggct tcccggtccg caagggcgat aaactggccg
    24961 gtacgcgcat cattcccttg gtcatcaaga aggataagat ggccgctgcc caggaagcag
    25021 ccggtccggc accgctcctc gacgtccatc ccttcggcca gaagaaagcc ggcgtcgtca
    25081 cgacggggaa tgaagtcttc catggccgta tcgaagatac gtttacgccg gtcatcgaaa
    25141 gcaagctgtc tgaatacggc gccgtcatgg ccatgcatac gaccctggcc gatgacccgg
    25201 ctgccattac ggcggttatc caggatatgc tggataaggg catggatatg gttttctgta
    25261 cgggtggcat gagtgtcgac cccgatgacc agacgccgct ggccatcaag aatacgggag
    25321 cccgcatcgt atcgtatggt gctccggtcc tgccaggggc catgttcctt ctggcttata
    25381 gcggggacga tcgccccatc gtcggtctgc cgggctgtgt catgtatgcc aagcggacga
    25441 tctttgacct cgtcctgccg tcgctcatgg ccgatgtccc cgtgacggcc gatgacctgg
    25501 ccggtctcgg cgaaggcggc ttatgcctgg gctgtcctgt ctgtaccttc ccgaactgtg
    25561 gctttggaaa gtaggtgatc ctggtgaacc cgtttacaca cattgacaac aacggtgacg
    25621 ccgtcatggt cgacgtcagc ggtaaagccg tgacggcccg gacggctgtg gcctgcggcc
    25681 gtatttccat gagccgcgag tgctatgaag ccgtcagggc cggtgccgtg aaaaaaggcg
    25741 atgtcctcgg cgtggcccgc attgccggca tcatgggagc caagcggacg agcgacctca
    25801 tcccgctctg ccatatcctc aatttgacca aatgcggcgt cgaattcatc ttccacgacg
    25861 atacttgtga actcgaaggc cgctgtactg tcaagtgcaa cggcaagacc ggcgtcgaaa
    25921 tggaagccct gacgggcgta tccgtggctt tgctgacggt ctacgatatg tgcaaggccg
    25981 tcgataagcg catgtgtctc aaggatatcc acctcgtcga aaaaatcggc ggcaagagcg
    26041 gccatttcca atatgacgga ggggagcacc atgaaggata aatggggacg gaccatcggc
    26101 tacctgcgcc tgtccctgac ggaccgctgc aacctgcgct gccgctactg tatgcctgaa
    26161 gccctgacca gcgtcggcca cgaagctgtc ctgcgctatg aagaaatgca gctcatctgt
    26221 cgggctgctc tgtccctggg catcgaccgc ttcaagatta ccggcggcga gccgctggtg
    26281 cgcaaagggg ccgtggcctt catggaatcc ctcaaagcca tgaaaggcgt ccgttctgtg
    26341 accttgacga cgaacggcct gctgttgtcc gacgccctgc cggacttggt ccgtatgggc
    26401 atcaacggca tcaacatcag cctggacagc ctcgatgccg ggcagtatgc cgggctgacc
    26461 ggaggcggcc agctgtccgt cgtcctggaa gccttggaac gggccgcggc tgctcgggta
    26521 aacgtcaaag taaacgccgt attactggaa gaaacgcagg accagatcct gccgctggcc
    26581 cgcttggccg gccggtttcc cgtcgatgtg cgcttcatcg aactcatgcc catcggctac
    26641 gggacgacgc tgaaaggcct tacggaagaa gcggccctgg ccgtcctgcg ccgggcctat
    26701 ccggatttgg ctgccacaga agaacggcgg ggaaacggac cggcttcgta ttaccggagc
    26761 ggccagctgc aaggtcgtat tggcttcatc gctgccaata cccatggctt ctgctcgtcc
    26821 tgtaaccgcc tgcgcctgac gagtacgggc tttttgaagc cctgtctctg ctatgacgat
    26881 ggcgtcgatt tgcgggccat cgtccgcagc ggccaagagg aggaaactat ctgtcaggag
    26941 ctgcagcgcg ccatggccag ggcggtttat gacaagccgg ccaagcattg cttcgccgaa
    27001 cagaaccata tcagtgaaca taagacgatg aatcagatcg gaggataaaa aatggggaaa
    27061 gtacatgctg tttgcatcag caccatccgc ggcgtggcta aacaggccgt accggaaggg
    27121 aatttcatag tcaactgggg cattgaaggc gacgctcatg ccgaacactg gcaccgccag
    27181 gtcagcctgc tgggcctgga aagcatcgaa gctttccgcc gccgcggcgg tcaggtggaa
    27241 ttcggcgctt tcggcgaaaa tctcgtcgtc gacggcttca atttctcggc cctgcctgta
    27301 gggacgctgc tgcgctgtgg cgacgtcctg ctggaaatga cccagattgg caaggaatgt
    27361 cacaaccatt gtgccgtcta tgaagccgtc ggcgattgca tcatgcccag ggaaggcgta
    27421 tttgcccgcg tccttgaagg cggcgtcatc cgcccgggcg atgtcatgac cgaagtcccg
    27481 cggcaaggga aacggcctta tcaggcggcg gttatcacct tgtcggataa gggaagcaaa
    27541 ggcctgcggg aagatgccag cggccccgtc attgtccagc ggctggaacg ggctgggttt
    27601 gaagtcatgg aacagctgct cctgcccgat ggcgagggcc ccttggaaca acagctctgc
    27661 cggctggccg accagcgcca ggctgacctc atcttgacga caggcggtac cgggtttggc
    27721 cctcgcgacg tcacgcccga agcgaccctg gccgttgccg accgtcaggc gccgggcatt
    27781 gccgaagcca tccgggccgc ttccatgcag attacgccgc gggccatgct gtcccgggcg
    27841 gtcagcgtca tccgcaagaa gaccttgatc atcaacctgc ccggcagtcc caaggcctgt
    27901 caggaatgta tggatgtctt cctggatacg attccccatg gcctcgactt actgcgcggc
    27961 gaagtccggg attgtggccg caagtaaatg catataaatg gatttacaaa aaggagatgt
    28021 acggtgatga cgtggaaaaa agtagtcgca tcggtcttgg cggcgacgct ggcttttgcc
    28081 ctggccggat gcgggtcgga caagaaagag gacaaagccg ccggggacgg cgataaagtc
    28141 cacctgaccg tctttgctgc ggccagcatg acggaaacga tgaaccagat tgccgcccag
    28201 tatcagaaag aacatccgga cgtggaaatt tcctttaact tcgattcgtc gggcacgctg
    28261 aagacgcaga tccagaacgg tgccgactgt gacctcttca tttcggcagc ccagaagcag
    28321 atgaaccagc tcgacgcctc aaaagacgcc aaagccaatc cggataagct ggacttcgtc
    28381 aagagcggca gccgcataga cctgttggaa aacaaggttg tcctggtgac gcccgaaggc
    28441 aaccccaagg gcgtccagaa tttcaatgat atggcggcga aattgaagga tggctccatc
    28501 cgcctggtca tgggcaacag cgacgttcct gtcggccagt atacacagaa aatcctgaaa
    28561 ttctacaacc tcgatgaaca ggccatcgct caggccggcc acattaccta tggctcgaac
    28621 gtcaaagaag tgacgaccca ggtccgcgag ggcagtgccg actgcggcgt catctatgcg
    28681 accgatgcct tcagtgccaa gttgaaacct gtcgacgaag cgacgaaaga tatgtgcggc
    28741 caggtcattt atccggcggc tgtcatgaag aacagcaagc acccgcagga agctcaggat
    28801 ttcctcaatt acctcaaggg ccaagaagcc atgaagattt tccagggcgt cggcttcagc
    28861 cctgtcggct aaggatagct gcttatggac tggtatcccc tttggaacag cctgcgcatt
    28921 gccgctattt cctgtgtcct cgtcttcttt agcggcattg ccctggctta ttatgtcgcc
    28981 cgcctgccgc gggtcctcaa agggctcctc gatgtcgtcc tgacactgcc cctggtcctg
    29041 ccgccgacgg tctgcggctg gctgctgctc ctggtcttcg gcttgaagca ccctgtcggc
    29101 atctttctca atgacgtggg catccgcttc gtcatgacct ggtatggcgg cgtcctggcg
    29161 tcggctgtcg tcgcttttcc cctcatgtac cgcacggccc gcggggcctt tgagagtttc
    29221 gacgaaaccc tggcctggag tggcaagacc ctgggcctgt cggatacgtt catcttctgg
    29281 catatccgca tgccttactg caagcagggc atcatcgccg ggacggtcct ggcctttgcc
    29341 cgggccctgg gcgaatacgg ggcgacgagt atgctcatcg gctatatccc caagaatacg
    29401 gcgaccattt cgacgacggt ctaccagttg tggcggacca atgacgaata cagcgccttc
    29461 atctgggtcc tggtcaacat cgccatcagc gccgtcgtcc tgctgacagt caatctcttt
    29521 gaaaagcgca gccgtgaaag gaggcgcctg gcatgatcga tatcgatgtc cataaggact
    29581 ggggctcttt tacgctggat gtgaagctcc atcatgagag cggcatcctc ggcctgctcg
    29641 gcgcttcggg ctgtggcaag agcaagactc tgcagtgcat tgccggtatt gaaaagcctg
    29701 atgcaggcaa agtcgtcgtc ggggcgcgga ccttgttcga cagcgggcgc catatcaatg
    29761 tccccgtccc gcagcggcgc gtaggctatc tgttccagca ctatgccctg ttccccaaca
    29821 tgaccgtcga agacaacatc cgctgcggcg tccgctcggc ttcgtccaag gctgacgccc
    29881 gccggcaggt ccgttccatg atggaacgcc tgaatttgac aggcctggag aaacagaaac
    29941 cggccgaatt gtccggcggc cagcagcagc gcgtggccct ggcccgcatc ctggtcaatg
    30001 agccggacat cctgctcctg gacgaaccct tcagcgctct ggacagttat ttaaaagaaa
    30061 aggtcatgac agaactcaag gacttgctgc gtcaattccc gaaagacgtc gtcttggtga
    30121 cacacagccg cgacgaagcc tatcaactct gtcagtccat cgccgtccta gacgacggcc
    30181 gcgtgtcggc ctgtgcgccg acaaaggaac tctttgccga tccccagtcc cgggtggcag
    30241 cggtcctgac gggctgcaag aatatcgtag cggcccgcaa ggccggaccg acagccgtct
    30301 tcatcccgtc gtggcagatt acgctgcaga cgggacggcc tgtcggcgac gacctctgtg
    30361 ccatcggcat ccgcggccac gccttttgcg gtggcgacgg cgaaaatgcc aggcccatcg
    30421 tcgtgaccga ggaaatcgag cagccctttg aatggatcgt caagttccgc tatgcccatc
    30481 aggaaggcgg gacgccggat atctggtggc gtttcgccaa gcccgaccgg ccggctgtcc
    30541 tgccggactg cctcactgtc gatgggcagg ctattttact cttatatagg tgatatcatg
    30601 gaagctttct atacccgatt atatgaaact ttgcagtctg gccagccggc tgtcctcctg
    30661 accgtcgtcg ccagccgggg ctcgacgccc cgcggcatcg gcagccatca ggccgtcttt
    30721 gccgatggga cgacggccgg tactgtcggt ggcggttatc aggaatatct ggccatccag
    30781 gccggacggc aatgcctgga gagccgccag tcggcttttc gccggctcat cctccacccc
    30841 aatgacgaag aagatatcaa cgctgcctgt ggcggggaat tgaccgtctt ctgccagtac
    30901 ctcgacccgg ccctgccggg cctgctggat gtactggccg ctatcatcca ggccctgcgc
    30961 cagaagacgg cgtcgtggct ggccctggat gtcagcgatg aagcccattg gggcatggcc
    31021 ctggtccggg aaggccaggt gcagggctgc ggccagatgg cggctttgca ggccgtcgat
    31081 gcggcttggt gccgtcagaa tgtagccggc cttgctgcgg atggccagcg gcgctggtac
    31141 agcgaacccc tgacttatcc gggacgggtc ttcgtctttg gcggcggcca cgtgtctcag
    31201 gccctggtcc cggtcctggc cagtatcggc ttttcctgca tcgtcgtcga tgaccgggcg
    31261 gaatttgccg atccggctct gttcccccag gcagatgccg tctataaagc tgattatgaa
    31321 gcccttccgg cggacctcgg cgtaacgcag gatgactatg tctgcatcat gacccgtggc
    31381 cacgtcggcg actacgatgt ccagcgccag atgctggcca tcaagccgta ttacctcggc
    31441 gtcatcggca gccgccgcaa attggccttt gtcaaagaga aactcctggc cgacggtttt
    31501 tcagctgccg aaatcgatgc ctgccacgcg cccatcggcc tggccatttc ggccgtgacg
    31561 ccggcagaaa tcgccatcag catcgctgcc gaactcattg ccgtccgggc caagcgggaa
    31621 gggcgggaaa agagccgcta ccgtaaacca tgacggccga ggtatacacc tatatcggtg
    31681 ccggcggcaa gacgaccagt atctttgccc gggcccggca ggaacgggct gccgggcgcc
    31741 gcgtggccgt cgtgacgacg acccatatga agcaccccag ccgatggttc gtaccggccg
    31801 ctttgccggc aggctggcag ggccaatggc ggcgcgacgg catcatcgtc gtcggccagg
    31861 aagggctgaa cgggaaaatc acctggcccg gcgaggatgt ttacctgcgt ttatgtgcgg
    31921 ctgccgacgt cgtcctcgtc gaaggcgatg gctcacggcg cctgcccttg aaggtcatgg
    31981 gcccccatga accggtcatc ccggccaaca gccgtgatgt atactgcctg gccggcctgt
    32041 cggccgtggg acggatcgtg aaggacgtct gtttccgctg ggagttattg catctcaatc
    32101 cggaagccgt cgtgaccgaa cgcctcctgg cctatattgt agaagccggc tgcctggccc
    32161 gcatcgggtc ctggaaagac aggacgaccg tcgtcctcaa ccaggccgac gatgaacggc
    32221 ggcagcaggc ggggaaacgg attttgagcg gcctgtcccg gccaggaata gtgacgacgt
    32281 atccagtgga aatgagggaa gcgaaatgaa aatacagtta gtcttgatgg cgtccggctt
    32341 cggccgccgt tttggcagcc ataaattgga agtgctcgtt catggacgcc ctttatatac
    32401 gtatggaatg aaaagcctgt gccaggcggc acaggctttt ttgccatctt ccggtatcga
    32461 ctgtcaggtg acggtcgtga cggctcatga tgccattgcc tcagcctgcc aggctcaggg
    32521 cattgacgtc atatggaacg gtcaggcggg agaaggtatg gctgcgtctt tgcgctgcgc
    32581 cgtccaggct catagtgaag ccgacgcctg ggcttttttt ccggcagacc agcccttgct
    32641 gacggcagaa acgattgccg acttcctgcg gcagttcctg gcctctggcg cagctgtagg
    32701 ctgtatgcat aacggcgagc ggcgctgcag ccctgccgtt ttccgccgcc cttatggacc
    32761 gggactcctg accctgcgcg gcgatacggg aggccggcag tttctgaagc gtcctcagac
    32821 ctgggtctat tatcccaaaa gaaaaggtga attatttgat atagattacc aggatgattt
    32881 aatcttttta aataaaacac tggataaata gattatgatt taagcgtatt aatattatag
    32941 tttacctcaa ctttttgatt gaacatattt ttgctatttt taattttaaa tataaaaata
    33001 taattaattt aggataaaac atttacaaat taaaatgtac gagtataata aaaggcagtt
    33061 gaacgattga ctatccggtc aatccatagg ggagtcaacg atactttaca atcataaggg
    33121 gagaggttct atgacgaaac acatctttag aaccaaaagt atcagtgaat ttatttcgga
    33181 aacgaaacaa gacggcggca tgaaccgcgt cctcggtact tttggcctga ccatgctcgg
    33241 tatcggcgct atcgtcggta caggtatctt tgttctgacc ggcgttgccg ctgccaatta
    33301 ttccggtcct gcacttatca tttctttcat catcgcagct ttggcttgcg gctgtgccgc
    33361 cctgtgttat gctgaatttg ccgccatggt tcccgttgcc ggcagtgcct atacttacgg
    33421 ctacgtcgcc ctcggtgaat tttgggcctg ggtcatcggc tgggacttga tactagagta
    33481 cgcctttgcc gtatcggccg tcgctattgg ctggtccggg tacttcaata atatcctcac
    33541 caacttgggc ctcgtcctgc cgaaagcctt gacactggct ccatataacg gcggcatcgt
    33601 caatctgccg gccgtcctca tcctctgcgt catcgccttc atcaacatcc acggcgtccg
    33661 ccagagtgca actgtcaaca acatcatcgt cggcatcaaa ttggccgtcg tcgccttgtt
    33721 cctggcttta ggcttcagcc acgtcgatgc tgccaactgg gttcccttca tgccctatgg
    33781 ctggtcgggt gtcttcgccg gcgcgtccat catcttcttc gcttatatcg gcttcgatgc
    33841 cgtttccaca gcagctgaag aagtcaaaaa tccgcagaaa gatctgcccc gcggcattat
    33901 cttatcgctc atcatctgta cggtcctgta catcgccgta tcggccgtcc tcaccggcat
    33961 ggttccgtac ctcgaattca agacgacagc agctccggtt gcctttgccc tccaggccgt
    34021 cggctatcac tggggtgctg cggctatctc tgtcggcgcc atctgcggcc tgacttcggt
    34081 cctcctggtc atgagctttg gccagagccg cgtactcttc gtcatgtccc gcgatggcct
    34141 gctgccgaaa ttcttcggcc acgtccatcc gaaatataag actccggccc gcagctccat
    34201 gctggtctgc gtggttacgg ctattacggc agggttcctg ccaatcaaca tcgtcgctga
    34261 aatgaccaat atcggtacac tctgtgcctt catcatcgtt tcggctgctg tcatcgtcct
    34321 tcgcaagaag aacccgaacc aggtacgcgc cttcaaatgt ccgctcgttc ccctggttcc
    34381 gctcctggct atcgccttct gcggtgtcct catctacatg ctgcccctgg ttacgcagat
    34441 ccgcttcgtc gtatggcttc tgttaggcct ggccatctat ttcggctatg gctacaacca
    34501 cagcatcatc tcccaggcac gccgcggaca actcggtgcc gctcccattc cgggtactgc
    34561 tgcaccggct gaacaaggcg tccacgaagg ccatccggct aaccattaat ccgctttcca
    34621 acccaatacc aatacagaaa aaggctatca ctctgtgaca gcctttttct atgttaaaaa
    34681 tgccccgact ggggctacgt aggggacgcc caaagggcgt cccgccgcga acagtcagcc
    34741 aatatatgta actggtcaat gaggcatggt tttgcgaact gcgaccggcg gccggcgttt
    34801 tcttggattc agatgcggct tcgccgcgac agactcaaac tgacaacgac gcttcgggaa
    34861 accctcagcg cgtagcggga cgccctctgg gcgtccccta cggcatcgca tacaggcgat
    34921 gaaaaattac gcggctatat gccgcggtcg taggggccgt cccaacgggc ggcccgccgc
    34981 gagatttgct cacagcccat tcgattatgg tggtatgttt ttacgaacca cgagccacga
    35041 accacgagcc actgattctg tgttatacta tggtgtatac ggtttatcat gaataaaggg
    35101 ggtctcctca tgaagtgtgt tgttttgctc agtggcggcg tcgattctac gacgtgtctg
    35161 gccatggctt tgacgaaata taaaaaggaa gacgtcttgg cgttgacggc ttattatggc
    35221 cagaaacatg ccaaggaatt ggaagcggcc cgcaaggtgg cggcttacta cggggtgcag
    35281 cagcaggagg tcaatttgtc tcaggctttt tctctgagcg attgttcgct cctgcagggc
    35341 agtcataagg cgattgccca tgaatcgtat gcagatcagc tggccaagaa gagcggcgaa
    35401 gggacggttg atacgtatgt gcctttccgc aacggtctgt tcttgtcgtt tgccgcagcc
    35461 gtcgccgtca gcgtcggcgc ggaagtcatt tattacggtg cccatgccga tgatgccgct
    35521 ggccgggcgt atccggactg cacaccggaa tttgaaacgg ccatgaacac ggccatttat
    35581 gaaggttctg gccggacctg ccacttggaa gcgcctttgt tatcgtggaa caaagcgggc
    35641 gtcgtcaaag aaggcctgcg cctgggcgcg ccttatgaat tgacctggag ctgctatgaa
    35701 ggcggcgaca agccctgtgg tgtctgcggg acctgccgcg accgggcggc tgcctttgct
    35761 gccaatgggg tcaaggaccc ggcattgtca taaaacaggg agtcagggag gagacatata
    35821 tatggaatat aaggtaaaga cgcggctgac gacgatggta ctgttggctc tcatggcctt
    35881 tgggtccgtc caggccagcg atgctgtgtc cgaaaaagat aatttttatc aggctgtcaa
    35941 tggcaagacg ttagcagctc agcaaatccg gccgacggaa gcttcttggt cctggttttc
    36001 tgaacggtct ttagaaaata agaaagccct gggcaaggaa ctggaaacgg tcgccgccaa
    36061 gcagggaact tatgccaagg ggacgccgga acagaaaatc gccgacctct acgcctgcgc
    36121 catcgacaat aaacagcgca acgccacggc tcgcgcccat ctgcaggaac tgacctgtcc
    36181 cattgaaaaa gcccagaccc tgccggaatt gacggcggcc ttgcaggctg tcagtgccaa
    36241 gaccgggacg gatatcttcg taggctacac cgtcgaccgc ctgccgacgg gcctgcgtta
    36301 tgtgccgcgt atcttgacgg tgacgccgtc ctttacgaag gatgaattgg aaaaagaacc
    36361 ccagcccgga gcctggaagg cttatcggga atacgtcgcc catatcctcg aagaagccgg
    36421 agaagcgccg gaccaggcgg cggctcacag cgaagccatc ttcgccatgg aaaagggcct
    36481 ggggccacac ttgctgacga gcgaacagcg caatgacgtg actgtccaga accgcctgat
    36541 gtcgcggggg aatctggaaa aactcatgcc caatatgggc ggcaagacgg tcctgaccaa
    36601 cctgggcctg aataaagaaa aacaattctt cttatcgaat ccggagtatt tacagcagtt
    36661 cgacgccctc tatgtgccta agaatctcga cctgctcaag agctatgctg tctatcaggt
    36721 ctatagcggc tttgcgccca gtgcggatat caaattgcgg gatctgcagc gcaattatgc
    36781 cctccagcgc ttcggcatcg cccagacgcg ggatgacaag gaaacggcga gccgcatggt
    36841 ccagaacatg ctgtcttatg aattcggcca gatttatatg aagaatcact gtacggcggc
    36901 catcatcaac gatgtgaagg ccatgatcaa tgaaatccgc gacgtctatc agcagcggct
    36961 ggaagccaat gactggctca gtcaggcgac gcgggccaaa gccgtcgaca agctcagcca
    37021 cctgcgcgtc tttgtcggcg gtccggctga agatgacaaa cctatcatcg aaagtatgcc
    37081 tgacgtcatt gctccacagg atggcggcga tttgctggcc aatgtcatgc acaacggcct
    37141 cctggaaaaa cagcaggtcc atgcgctatt gggaacgaat tttaatcccg ataaatggta
    37201 tgcctttgac ccgcaagatg tcaatgccgc ttatatcccg gaaaataaca gcatcaccat
    37261 cccggccggt atcctgcagc cgccgttcta cgatgccaaa gcttcacgcg gtgccaatct
    37321 gggcggcatc ggcgtcgtca ttggccatga gatttcccat gcttttgacc cgaatggcag
    37381 caagtacgac agcgaaggcc gcttgaagaa ctggtggaag gcaaaagact atgaagcttt
    37441 ccagaaatta tcggctgctt tcggtcctta ttacgatcgc tataccctcg gcaaggggct
    37501 ccatgaaaat ggcaagctcg tcagcaacga agccattgcc gactgcggcg gcctgtcggt
    37561 tgcgacggaa ctggcccatg acgaccaagg gatgctgcgc gacttatacc gcacctttgc
    37621 cacggtattt gcgaccaaga tgaccgacca gctgctgctc tacctggtcc agaatgatcc
    37681 ccatccgact ggcgaagccc gcgtcaacgg cgccttgtcg tcgacgaatg gcttctatac
    37741 ggcctatggc atccagcccg gcgacggcat gtatgtcgaa ccgcagaagc gcgtccattt
    37801 gtggtaaaat cttcgtggct agtggctagt ggctagtggc tagtggctag tggttcgtaa
    37861 ggccgtcctg ctgcggcggc ctgcggcctg tgtcctcata aagtatttac acaactaatc
    37921 cttgcattat cgtggggaat acggtacaat cgcagtgagc aatagccgta tattgcacta
    37981 gtgggacaaa aaacgtacca ctaggaacag aaacgtcaat cgttacgtgt acgtgagtag
    38041 aaaaggagaa atctgtatgc tgaaaaaaac aaagatcgtc tgcaccttag gccccagttc
    38101 caataccccg gaaattatcg aaaagatgat taaagccggc a
//
LOCUS       DAWCYS010000002        33891 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_70_length_33891_cov_11.989686, whole genome shotgun sequence.
ACCESSION   DAWCYS010000002 DAWCYS010000000
VERSION     DAWCYS010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 33891)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 33891)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..33891
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_33891_cov_11.989686"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8264 a         8651 c         9088 g         7888 t
ORIGIN      
        1 cggccgatga aatcacggct acggccaaga aattcatcgc cgccgtccag gctgcccgga
       61 agtaaaaatt tgatgtaatg tatttgatga aaggctctcc tcataggaga gctggccgcc
      121 gaaggcggtc tgagaggttg ctttaaaaac gggactggcg catgaatgcc tatgtcatca
      181 tgcgccagtc ttttttcttt aagtttgtac ctctcgatga atttacgcgt tcacataatt
      241 cgtttaaatg gtgtgatgac ataaaagaaa aactcgataa ttaagccgct cacagtcgtg
      301 taaatcaaaa taaattaatt tactgatgct ggaaacagta ctttttttct tctggaaata
      361 tataatttgt gcccgttttg tgataagatg aaattgagaa aagcaggtat tgatgaagtg
      421 tcgccgcaca ctcgtgactt tagtcatgag ttaggcgaca cataacatat gtcattttaa
      481 aatcgcaaat aaaaccttga aatcattgag ctttccaact gcatatgtta ttatatatgc
      541 aggaggtgag aatatgacaa tagaaaccca tcatggtcga ggatatgtct attgtttaca
      601 ctaccatatc gtttggtgtg tgaagtacag acataaggta ctgacaccag aaattgtcgg
      661 agaccttgtt cagatactag aaaaaatagc caatgacaat gacttcgaaa ttttggaact
      721 caacacagac acagaccatg tacatattct catagactgt tccccgcagc acttcatccc
      781 agacataatg aaagctatta aaggcgtgtc tgctcgactg cttatgaaaa agtacggtga
      841 tacgctcaga aagaagctct ggggagggca tttgtggaac ccgtcttatt ttataacgac
      901 tgtttctgag aacacagaag cacaagttag ggaatacatc aaatctcaag gaaaaaagga
      961 ttaagaaatg agggcttggc gatgaagata atgactactt acaaaaccaa aatcagagat
     1021 tcgcatagtg cattagctaa caccgtggga atctatcgtc aagctgtaga ctttcttatt
     1081 ggtgtggctt tagaagaatg gaatgccctc aaagaaatcg aacgctctct cgaaaaacag
     1141 agggcgattg agtgcgtaat tcatcatact aaatctaatc cttgcccaaa atacgatttc
     1201 gattccagat ttggcaaatt cccaagttat cttcgccgtg cggctatcac cgaggctgtc
     1261 ggcaaggtgt cctcctacaa aagcaactat gctaactggg aagaaaatcc gcaaggtaaa
     1321 gaacctggga ttccagttgc agggtatatc taccccgcta tgtatcgaga caactgcttt
     1381 gttcgtattg gcacatatac agcaagacta aaagtctgga ttcgaaatac ttgggattgg
     1441 ctggatgtgc agcttcgtaa atctgacgtt gactatatcc aacgtcattg tgcaacccgc
     1501 aaggagtgcg taccgactct tcgtagacgg gggaagaact ggtatttgga ttttaccttc
     1561 gaggaaaatg tcaaactcga agacaaaccc attgaccgtc aaattatcat cggagtagac
     1621 cttggcatta acaatgcttg tacttgttcc gctatgttat cagatggcac tgtcatcggc
     1681 aggaagattc tttccctgcc gagagaaaaa gactctctgg ggcataagct gaacaaaatc
     1741 aagaaggcac atcagcatgg tgcgaggaaa atgccgaggt tatgggcaaa ggcaaaaggc
     1801 gtaaaccttg acattgccca taagacggct cagttcatcg ctgatgtagc tatgctctat
     1861 tccgctgacg taattgtatt tgaacatctc gacactcagg gtaagaagcg aggctcaaag
     1921 aagcagaaac tccatcattg gcgggcacaa gctgtgcagc gtatcgttgc cgacaaaacc
     1981 caccgtgatg gagttcgcgt ccgtcgtgtt tgtgcatgga acacatcaaa actcgctttc
     2041 gacggttctg gtgaggtcga gcgagacgaa cacaattatt cgatgtgtac tttcaaaact
     2101 ggcaaaaggt atcattgtga cctgtctgcc agttataaca ttggtgctcg gtattttatt
     2161 cgagaaatcc taaaatcctt gcctgcgaag gcaaggttgg gcattgaggc aaaagttcct
     2221 caatgtacca agagaaccac ctgcaccttg tctacgttac ttagtctgaa tgcggagctt
     2281 gtggcgtaat gccatcaagt tatctgagtt cagtgcctta tcgtagaaag acagtccgct
     2341 gtcctaaaga cagcatgaca cgaacttaat cgtgtcgttg gaagcatccg actttagtcg
     2401 ggtgagggtt cactttcaga atgatggagt tgttatggaa aatttggaat tatttgaaaa
     2461 agcgccgaat gaaacggtgc gggaatacgc ctatcgcgtg ttgtatgaaa acattatgtc
     2521 tttgaagctg ccgccaggta cggccatgtc tgaacaggaa ctgtcgggaa tcttgcatgt
     2581 cagccggaca ccggtgcggg aagccttcat ccgcctgtcg caaaaggggc tgctcgaaat
     2641 tttaccgcag cgcgggacct tcgtatctaa gattcacacg gaacagctgg cagagttccg
     2701 ctttttccgg gtcaccttgg aacgggctat cgtagaattg gcttgccagg attttccgga
     2761 tccgtggcgg acgaaattag aattctgtat tttcgagcag ggcaaattca tcatctcaaa
     2821 agatgcagaa aaatttttca agagtgataa taccctgcac tctctacttt atgaaggctg
     2881 tgggaaaccg catatctggc agattgtcca agaatcgaac ctggattatg cccgggcccg
     2941 cgtcctcaac gtcagcgatg ccgcagagca gatgcatctt ctctacgaac agcataaaag
     3001 catagtcggc gctatctttg ccggtaatac agaactggca aaacgcatca tgaccgaaca
     3061 cattgacaag gtcatggggg atgtagaagt gctgaaaaaa gaatatccag agtatttccg
     3121 gtaaaggtaa agatagtttg tagttcagag tttgtagttt gcagaagatg actttaaatt
     3181 ttttaccatt tgttgcaaac taaaaactac aaacttaaaa ctcgaaaaga ggctgtcgca
     3241 catgcgacag cctctttttt cacctcttga aactttcccg ttctacgagg tcggcactga
     3301 attcggtgcg gaccgggacg gaatcggctt cttttccagt cagctcggtg acgatgtgga
     3361 cggcagcatt gcacaggctg gtcagtttgt tgtccaggct ggtaatggtc ggcgtggcac
     3421 attgggccag gatggagtta ttgcagccga tgacgggaat gttttttcca tggcgcaagg
     3481 cggcacgctg ggccgcaacg gcaaagatgt cttctgacgt gacgatggcg tcaaagggaa
     3541 tatcgtctgc gactaaacgg tcgatgagtg cttccgtatc ttgcaaggtc cggggaatct
     3601 gctggatgag ttctggtttc tccgtcaatc tccattcccg gatcccctgg cggaaaccgt
     3661 cgagcttatg gcggccgctg taggtgaggg aatcgtaaat gtagaggatg tcctgacagt
     3721 tcttggcagc caaggcggcg atgaccattt tcatgccttc tttttcattg cagacgacgc
     3781 tgtacatatt gggctgatgg taatcgctgt tgatggtgat gaccgggatt tgccgggccg
     3841 tttcacagag ttggcggaca tcggattcgg aagaaaatgg cgtgccgata gtgatgattg
     3901 catcgacgtg cttatcggcc atagcggaaa tatatttgcc gttatgccct gtatcgctgc
     3961 cggtattgga gagcatgacg tcgaaatggt gcttccgcag gccatcttcc agcagggata
     4021 cggcatgggc atagaatata tcggccacgt cggtacagat gacgccgacg attttcatgg
     4081 aatcgagacc taagccccgg gcgaagggat tgggtttata gcccatttct ttcataactt
     4141 gaaggacttt cgcccgtgtc ttttcactga cccgtgtgct gccgttgagg acgcgggaga
     4201 cggtggcaat cgatacattg catttctggg cgatgtcgta aatggtgatg ttcatggcga
     4261 tcgttcctct ttatgtaaaa aatctggatt gcttacatta tagcatactt ttttctggaa
     4321 aaaggcgggg atacgaaaat gtaaaatgta agcgcttaac caagcggaat aggaaaacag
     4381 gaaaatctag gcacaaaaag aataataaaa tggatcgacc attcataatt atgagttaaa
     4441 agcatggaaa atgattaatt tttcaaagtt aatacggaaa aatcatatta cgttgacact
     4501 taggtaatga catgatattc tttcagtgaa gataaatgta agagcttaca ataaaattta
     4561 ccaatgatcg atgaagttag cggccttata ttgggaggag acggacaaaa tgaaacaatt
     4621 catggacaaa gacttcttat tggatacggc taccgacaag cagttattcc acgattatcc
     4681 agaacatatg ccgatcattg attaccactg ccatatttct ccgcaggaaa tcgcagaaga
     4741 tcaccatttc cgcagcatta ccgaagtatg gctcggcggt gatcactata agtggcgcct
     4801 cattcgttcc aatgggacac cggaagaaaa ggttaccggg acggaatcga cagatttgga
     4861 aaaattcgtt gaatatgcga aagctctttc acgagctatt ggcaatccgc tctatcattg
     4921 gagccatttg gaactgcagc gctatttcgg tattaccgaa ccgttgaacg aaaagaacgc
     4981 tgaagatatc ttcaatcgct gcaatgccaa gctgcaggaa aaaggcatgg gcgtccaggg
     5041 cttgattgaa cagtccaatg tcaaagtcat ctgcacgact gatgacccgg ccgatgattt
     5101 gaaatggcat cagaagctga aagaagaaaa gacgtgcagt gccaaggtat atccggctat
     5161 gcgtccggac aagctgatga acatcgacaa agccggttat gccgattaca tggcccgcct
     5221 cggtcaggct accggcgttg aaatcaagac ctttgccgat atccgccaag ccatggacaa
     5281 gcgcatcgcc ttctttgaag aaatgggctg ccgcgctaca gaccatgcac tggaatatgt
     5341 cttctgccgc gaagccgatg aagcgactgt cgatgctatc attgccaaag ccttgaaagg
     5401 cgaaagcatc agtaaagaag aaacggaagc ctataagacg gctgtcctca cctacctggc
     5461 tggtgaatat tacaaacgcg gctgggtcat gcagctccac ttcgctaccc tgcgcgacct
     5521 caattcggaa atgtaccgca agttaggacc ggatacgggt tttgacagtg ttgccgaagg
     5581 gaattgctgc aaaggcctca tcgccttcct ggatcatctc aatgaacgcc atatcctgcc
     5641 gaagacgatt ctctactccg ggacgccgaa cgataacgcc atgattggca ccattatcgg
     5701 cgctttccag ggtcctgaag cccgcggcaa gattcagcag ggcgctgcct ggtggttcaa
     5761 tgatacgaaa cagggcatga tcgaccagat gacgagtatt gctaacttgt ccgtctttgg
     5821 caacatcctg gggatgctga ccgactcccg gtccttctta tcctatgccc gtcatgaata
     5881 ttatcgccga atcctctgca actggatcgg caatctcgtc gaaaacggcg aatatccgaa
     5941 cgatgaagac gcactccgtc agctcgttga agatatctgc tataacaacg tagccaacta
     6001 cttccagttc caaatgtaaa cggagagtaa acgatgaaaa acgtcagtga actttataca
     6061 aaagccaaac atcccgtccg tatcttacag ttcggagaag gcaatttcct gcgggccttc
     6121 gtcgattacg ccgtcgatgt agctaatgaa gaaaatggtt ttgatggcag cgtcgctgtc
     6181 atcatgcccc ggtcgggcaa gacggaccgc tttgctaaac aggatgatgt ttacacggtc
     6241 tgcctgcgcg gccagcagga tggcaaagtc tataaagaaa accgggtcat tacttccgtc
     6301 cagaccgtgc tcagcgcccg cgatgaatat gatgcgttca tggccctggc tcatgaagat
     6361 tcgctggaat tcgtcgtatc gaatacgacg gaagccggta tcacctttga tgaaaaggat
     6421 gcttttacag attgcccgcc gtcgactttc ccggcaaaat tgaccaagtt cctctatgaa
     6481 cgctatacct tttataaagg cgctgccgat aaaggcctga ccatgctgcc gacggaattg
     6541 aatgacaaca acggtaaact ccttcgggac tgcgtaaaca aatatgtatc cctgtggaaa
     6601 ctggaagcgt ctttccattc ctggctcgac cagcactgcc agttcacgga taccttggtc
     6661 gaccgcatcg tcgtcggcta tccggctgat tccatcgcgg ctctgcagga cgaactgggc
     6721 tatgaagaca gcctgctcga ccaggccgaa cctttctcct tatgggttat cggcgacccg
     6781 gcgctggctt cgcggctgcc gttaaactca agtaaattcc atgttgaatt cacggaccat
     6841 atccaggcct ttaaagaaca aaaagtgcgc atcctcaacg gggctcatac gtctatggtc
     6901 ctcggtgctt atctggccgg tctcgattat gtcggccagt gcatggaaga cccagtcatc
     6961 cgcaagtccc tggaccagac cgtcttcgat gaaatcgttc cgaccgtcca tttgccggaa
     7021 gaaaaggccg ttgcctttgc caaggctgtt tatgaacgct ttgaaaatcc ttttgtcaaa
     7081 catgcattgc tctcgatttc cctgaattcc atttccaagt ggaaagcccg catcctgccg
     7141 acctttaaag acagtctggc agcgacgggg aaacttccga aatggctgac ctattccttt
     7201 gccgctttcc tggctttcta tcggacgacg gaggctggtg aacattgtct tatcggttca
     7261 cggccgggca atacttatga aatccacgat gatgaagata aactggcctt tgtcaaagaa
     7321 caggccggta aaacgacggc tgattacgtc aaggccatca tggcacgcag tgattggtgg
     7381 ggcgaagacc tgtcggctat cgacggcttt gccgatgccg ttacggctca tctggaccgc
     7441 atggctgaaa tcggtgtcaa agctcacatt gccgaattga caaaggagta aacgatgaac
     7501 gtattccaaa tcaactctca tgataatgtc gccgtagctg tcgatgccgt tgccaaaggc
     7561 agcgtcgtca cggctggcgg taagactttc acggtctgcc aggacatccc ggcagggcat
     7621 aaagtggccc tgcagccgat tgccaaaggc gaagacgtca tcaaatacgg ctttcccatc
     7681 gggacggcta aaacggatat ccccgtcggt gcctgggtcc atacgcagaa cgtccaaagt
     7741 aaactcggtg atttacttca ctatacctat gaaccggaaa aagctgatcg ttctccctgg
     7801 aaaagtgaaa aaaaatacga attccagggc tataaacgga gtgatggcag tgccggcatc
     7861 cgaaatgaag tatggatcat cccgaccgtc ggctgtgtca acaatattgc cagagccatt
     7921 gaaacggcag cccaggctta taagacggaa aatatcgatg gtatctatgc ctatagtcat
     7981 ccccacggct gctcgcagtt aggcgatgac caggtccata cacaaaagat actaagtggg
     8041 ctgattcaca atcccaacgc cggtgccgtc ctggtcctga gcctgggctg tgaaaacaat
     8101 caggtcagcc tgatgaaaga agtcatcggc gactatgatc cggaccgcgt caaattcctg
     8161 gtctgccagg acgtggaaga tgaaattgaa gccggcacgg ctatcgtcaa agacctctgc
     8221 cattacgcgg ctcagttcca tcgccagccc tgcgatgcat cgctgctgac tatcggtttg
     8281 aaatgcggcg gctccgatgg tttttcgggc attacagcca atcccctggt cggggagatt
     8341 tccaaccgcc tcatcgcagc cggcggtacg tcgattctga ctgaagtgcc ggaaatgttc
     8401 ggcgccgaaa cgctgctcat gaaccgggct cgcaatcggg aagtcttcga caagaccgtc
     8461 gacctcatca accacttcaa agaatatttc atgagttaca gcgaaaaaat caatgaaaat
     8521 ccatcgccag gcaacaaggc cggcggcatc acgacgctgg aagataagtc cctgggttgt
     8581 gtccagaagg gcggccgggc tctcgtcgaa gatgtcctgg cctatggaga cagggcgacg
     8641 aaaaaaggcc taaacttgtt gcaggctcca ggcaatgatt tagtagcctc caatgcgctg
     8701 gccgctgccg gagcccatat ggtactgttt acaacgggcc ggggtacgcc gtttgcctgc
     8761 cccgtaccga cgattaagat ttccagcaat agtcacctgg ccggtttcaa acggaactgg
     8821 atcgatttca atgccgggac cattgctgaa ggggaatcgc gggaagccgc agcagaccgc
     8881 cttttccagt atattcttga cgtagcgtca ggtcgtgtcc atgcgaaatc ggaagccttg
     8941 gacaagcacg aactggctat attcaaaaac ggagttactt tataaggagg gagtgctatg
     9001 agcacacaag cagaggcgtt gccgaacaag acggagaccg ttacgccggc agcttctaaa
     9061 gaaggccatg ttaaattatc gtggcgtgaa aaaatctgtt acggtttcgg tgactttggg
     9121 aacggtttca tgttcgactt aggccaggcc tatttgacga aatattggat tgatgtctgc
     9181 accattccgg cagctgccgt tgcaggtatc ttcgccttta cgaaaatctt cgatgctttc
     9241 atggatccta tcgccggttc cgtcatcgac ggccgcaaga atatcggccc gcgcggcaag
     9301 ttccgcccgg ttatgatggc ttcggctgtc atcctggcta ttttgacggt cattactttt
     9361 accatgcccg atgtatcgcc gacgggcaag atactctttg cctatggtgc ttacatggcc
     9421 tggggccttg tttactcgtt tactaacatc ccttattctt ccctggccag tgtcatgacc
     9481 cgcgatgtcg aagaacgaag tcagatggcg acgacccgcc aggccggttc tatcggggcc
     9541 cagctcatta ccggcatggc cttcgtaccg gtcgtcctca tgttcgcttc gccccatgaa
     9601 ggcttctttg ctgcgtctat cgtcatgtct atcatcggcg tcatcggttt tgccatctgc
     9661 tatttcaact gccatgaaca cgttccggtc aagcgcaata cccagaatga acagaaagca
     9721 aaattctcgg attatattaa actcgtgttt actaacaaac cgcttttatg cattattttg
     9781 atgactttgt ttaccatctc ggctatgaac acgaataacc agatgatgat tttcttctgc
     9841 cagtataact taggccatat gggcttgcag ccgattgtaa acggcatcat gatgggctgc
     9901 tccgtcgtag gtatcctgct catcccgaaa ctggtcaaga tgttcggcaa gaaaaagacg
     9961 gccatcggcg gcttgcttat cggctgtgcg gctgacctct tgaacttcgt catcccgact
    10021 aatatttaca cctttatcgt attggtcacc atcggttacg tagccctggc tattccgaac
    10081 ggcgttacct gggctttcgt ttccgacgtc atcgactaca gcgaatggca caacggcatc
    10141 cgcaaagaag ggattaccta tgcggctttc aacttctccc gtaaaattgc ccaggccctg
    10201 gccgctgtcg taagttcggg catcctggta ctgacaggtt acgtcgccaa tgccgttcag
    10261 agtgaaatga cattgatggg gattaaagca gctatgacct tgtatccggg cgttgccctc
    10321 ggcattgctg ctatcatcct ctacttcttc tatggcttga cagacgacaa attcaagaag
    10381 attgccgatg atctgaacca tggtaaatgg gaacacggca cgattgaata aggaggcttt
    10441 gtcatgaaag ataagaactg cggttactgc gtaaaaggcg aaccgcttgc taaattcggc
    10501 atttacatct gcgacttatc ggtcagtatg ctggtcctgt tcaaagaaca gagtcatccc
    10561 ggccgctgca tcgtcgccta taaagaccat gtcagtgaaa tgacggacct gagcgacgaa
    10621 gaacgcaatg ccttttttgc cgacgttgcc aaggcggcga aagccatcca tcaggcattc
    10681 catccggata aggtaaacta tggttcctat gccgatacgg ggcatcatct ccacgttcac
    10741 ttagtgccga aatacaaaga tgattttgaa tggaacagta cctttgccat gaatccggac
    10801 cgcgtctatt tgacggatgc cgcctatgaa gacatgattg acaaaataaa ggcacaattg
    10861 gaggaaaacc atgagtaaag tgcttactat cggtgaagcc atgggcttgc tgattgctga
    10921 agaaatgggg ccgctggaag aagtggaaca tttttcccgc catgtctgcg gggcggaact
    10981 gaattacgcc gtcggcatgg cccggctggg caatgatgtg tcgtatattt ccaaggtcgg
    11041 cgtcgacccc ttcgggaagt gcatctgcaa cttcctcaag gccaatggca tccacgatga
    11101 ctatgtctgg accgatgatg accatatgac ggggttccag atgaaagaaa aggtcctgga
    11161 aggggacccg acggtagcca atatccgcaa acacacggcc ttttcccact tccgaccggc
    11221 ggacctctcc ggtatcgatt ggaccggcgt cgaccacctg cacgtcacgg ggattcctct
    11281 ggccctgtcg gaatcgtgcc gggaaacgat ttatacgctg atgatgcggg ctcacggtaa
    11341 gggcgtgcgc atttcctttg accccaacct gcggcccatg ctctggaagt cccaggaaat
    11401 catggcccgt gtcatcaacg acgccgccca gtatgccgac atcgtcatgc ctggtctcaa
    11461 tgaaggccgg atcctgacgg gaatggataa tgaaaaagat atcgccggtt attatttgca
    11521 gcgcggcgtc cagaccgtcg tcatcaaaat gggcggcagc catgggacct atatccgcac
    11581 ggccgatgac caggaatacc gcgtgccgag ctatcacgtc gaccacgtcg tcgatactgt
    11641 cggcgccggt gacggctttg ccgtcggttt caccagtgcc atcctcgatg gactgagcct
    11701 ggaagatgct gcccgccagg gtgctgccat tggggccatg gccatccagg tcgccggtga
    11761 taacgaagga ttgccgacgc gggaacaact ggcggtattc caacagaacg catgatgttg
    11821 gttgtgggat tcccacaagc gggccgccct ttgggacggc ccctacgtgc ggtcctatgg
    11881 gaccgctgtg gcttgcgacc tgcgttaatg acccctgcat caatctgtat gattggtgca
    11941 aaacaacccc attaaccgtg aataggacat ggccgataaa tgtggaacgg gcgccccacg
    12001 tggcgcccct acgaaaatgg gtataaaaac atgtaggggc tcgcacgtgg cgagcccgcc
    12061 cggaatgatg ggggtaaaac gttcatccgt cgtgaaaagg agagaaaatc atggataaat
    12121 tagatgttgt taagaaaatt caggaaatcg gcgtcgtcgc tgtcgttcga ggcaagacac
    12181 cggaagaagc gatgatgatg tccgaagcct gcatcaaagg tggcgtcacg gccattgaac
    12241 tggctttcac gacgccgcgg gctcatgaag tcatcgaagc cctttccaag aagtatgccg
    12301 ataatcctga cgtcctcatc ggcgccggta cggtcctcga tgccattacg gcccgcatcg
    12361 ctatcctcga cggcgcccag ttcatcgtat cgccggccct ggatgtagaa acgattaagc
    12421 tctgcaaccg ctatcgcgtg gccgtcatgc cggggacgac gaccctcaac ggcgtcatta
    12481 cggccctgga atacggcgct gacgtcgtca agatcttccc tggggaaatc ctgggcatga
    12541 aagccatcaa ggccatccat ggtcccctgc cgcaggctcc gctcatgccg acaggcggtg
    12601 tcaatgtaga aaacgccggt gactggatca aagccggctg cgtcgccgtc ggtgccggcg
    12661 gtgccctcac aggcggcggc aagacggccg atgaaatcac ggctacggcc aagaaattca
    12721 tcgccgccgt ccaggctgct cgaatttaaa agtttatagt ataaagtttg tagtttgtag
    12781 ttttttggtt ttaaattttt acccatccac tacaaactaa aaactgcaaa cttaaaactc
    12841 aaaaagaggc tgtcgcgcgt gcgcagcctc tttttttaca attgtttaca cgcaaaggaa
    12901 aacgtttaaa tagagaacac actcgtttta aaaacgtgat aaccaagcgg gcgacaaata
    12961 tttaaatcaa aataaataag tttaaaatag ttcaataaaa catataatat gtaaccattg
    13021 actttacaat aaaaatgtcg tatgataggg atgtaatcag gataattcta tcctggatgt
    13081 tttgattcgt catttatagg aggtttttta aaatggatgc aaaaacagta gaattcgtta
    13141 cggctaagac acatgatttg atggcagctc ccagctgcaa tccgacgctg aaaacggccg
    13201 ctcaggcttg gctcgatgct gccgataaag acgcagctgc gccggcttat atcgaagcct
    13261 tgaagcaggg cgtttcgacc atcgatgaac tcttggcctt tgcagcttcg gaacatgctg
    13321 tccagattat gggggccgat gcagcgaaac agctcttggc tcacgccaaa gatattcagg
    13381 ccaaaggggc taaattctgt gactgcccgg cttgtacggc tgacctggct atccttcgtg
    13441 aattaaaagc catttaagga gtctttcata tgcagatttc cagtcgattt accatggccg
    13501 tccataccct gctgtgcatc gatttcttcg gcaaaacgga aaaagtgacg agtgatttca
    13561 ttgctgccag cgtagggacg aatcccgtca tcatccgcaa actgcttatc cagctcaaag
    13621 cggccgggtt gatccaggtc aagcgcggga ccggtggtac agcgttggaa aaaccgattg
    13681 ctgacattag tttatatgac gtttaccatg ccgtagattg cgttggcggc gactcgctct
    13741 tccatttcca cgaacatccc tgcccgcagt gtccggtggg caagacgatt catgctggcc
    13801 tggacgacag gctgcatcag gtgcaggatg ccatggagga taagatgcgc agcatctcct
    13861 tggccgatgt agcgggaacg atgcgggacg tctttgcaaa acagggctga gtccggctga
    13921 aagagaggct atcatggcaa ggtctatctt tttattcgct caatggttca ttgcccggcg
    13981 cagaacctgg cttcggatcc ttacctggat tacccggttc ggctactggc tgttcatcct
    14041 ttatggcctt atcgaatggt tccggccgcg gccggtttca gagcagatta aacgtcggaa
    14101 gacattagtt tactgtctct tttccgtcat cgtgggctct tcggtttcct gggtcatcgg
    14161 taaaatctgg catcgccccc ggccttttgc ctgtggtgac gcggcgccgc tggtagcgca
    14221 taaggccaat gcgtcctttc ccagcaatca ttccatgaac agtctggcca tttccctgat
    14281 gctactggcc cggcacaacc cctggggact gccggccctg gcctggacag gcgtcctggc
    14341 gtcgtcgcgg atactcagcg gtctccatta cgtcagcgac gtcgtcggcg gcttcttcat
    14401 cggagctgcg gccagccatg tcgtttacca tagtaaacgt tgttcacaac tggctgatca
    14461 catcctttac tattggcacg tcggggccac ggtcgtacag acttggtggc gcaagtgctg
    14521 atgggctgat aggtgcacgg agactttaaa tttaaaacca tccgctaact gctaacggct
    14581 atctgctgac tgctaaaaaa agagctgtct tcataggaca gctctttttt tattccaaac
    14641 tgtcatagca tcgccacatt tctatggtat actaaagcag aaagaaatgg ttttaattaa
    14701 aactattttt gaggaaaggt gagttgtgat atgcgaggta gaaataagat gtgcgcagca
    14761 gcgctgtttt tcatgatgac tggcggcctg gtgttggcgg cggatacgac ggaagcagaa
    14821 ccggtacaag ccgtagcggc ggctacgcag actaaggcta aaacggccca ggctgctccg
    14881 gtgcagcaga cgtatagcct cgatttggat acgacgaagt atgaaaagaa aacgatgaca
    14941 gtcgacggta agaaagtggc tttccgggcc tatgaaaacc gggtgtatgt ggcccatcct
    15001 gtcgatacga cataccagtc catgaatata tacgttcccg aaggatactt taatggcaag
    15061 acgatcaacg gctatacggc gaagacagct ccgattttcc tgcccaatac agtcggcggt
    15121 tatatgcggg ggcaccgggg cagccgtctg aaaatgatcg gatgactggc ggtgccaatg
    15181 ccatgctcgt cgccctctcc aaaggctatg tcgtcgcagc tccgggagcc cgtggacgga
    15241 caactcaggc tgctgacggc acgtataccg gcaaggctcc ggcacttatc gtcgatatga
    15301 aggccgctgt ccgttatttg cgccataatg cagggaaact gcctggtgat acggaaaaaa
    15361 tcatttctaa tggaaccagt gccggcggtg ctttatcgac gctcatcggg gcgacaggcg
    15421 acagtaagga ctatgaaccg tatttgaaag cccttggcgc ggctgatgaa cgggatgata
    15481 tttatgcttc cagcgtatac tgcccgatta ccgacctgga ccatgctgac atggcttatg
    15541 aatggatgtt taatggtgta aacacgtatc atcagggaaa tatgggtgct atgaaaccgc
    15601 cggcaggtaa tagccaggct aaaccggcag gcgtcgttac caaccggcct gacaatgcac
    15661 cggtcgaagc cgcagccggg acggaaatga ccgctgtcca gcaacaggca tcgaaggatt
    15721 tgaaagccat gtacccggct tatatcaata gtttacatct cgttgacaag aatgggaaag
    15781 ccttgaccct cgatgaaaat ggacgaggct cctttgccga ttacattaaa tcgatttaca
    15841 tggcttcggc acagaaagcc ctcgacaacg gcaccgattt gtcttccaag acatggctga
    15901 ccatcaacga cggcaaagtg accgacatgg atttagccgg ctatgctgtc gatgtaaccc
    15961 ggctgaaggc agccccggcc tttgatgcgc tcgatatgag cagtgctgaa acggaagagt
    16021 tcggcacggc aactgtcgat aaacagcatt ttacagtata cggcaaagac cattcgacca
    16081 tagccggatc ggctctggcc gataaggaca tcgtcaagac gatgaatccc ctgaactaca
    16141 tcggcaaatc cggcgtcaag acggcgcatt actggcgtat ccgccacggc gaagccgacc
    16201 gcgatacgac cattgccgta ccggcagtcg tcgccctgcg tctgcagcag gccggttatg
    16261 atgtcgattt tgcctctccc tgggcccgcg gccatgccgg cgattacgat ttagacgagc
    16321 tcttcgcctg gatggatgcc atcggtcata aaaactagtt taccagagat tatcttcttc
    16381 gttaaacttc catgtaaacg gttcggatgt gattgtcatg tccggatcgt tttttatgtc
    16441 ttctaagcac gagacggaac attcgatgtc ttccagtttc agtgtgttct ttatgcggac
    16501 gattttagcc tggttcgggt cggtcaggaa ggcgtgggcc atggccattt cgatggcgtc
    16561 tttatcgctg ttgccataca ggggcaggag ggccatgcgg aaggacttgg ccgtaatgca
    16621 gttggtataa acggaagcga aatccatctt gtttacgaag cggtaactga cgatgtcggc
    16681 cataccgcag ccggcaccat tgccatggga agcctcggtt atatccagga tgacgatgcg
    16741 ttcgatgtcc ggcgctaagg cgcggaaggc cggttcctga ctgaagcgtt ccgtccgtcc
    16801 cgtgatgttc gggtccatgc cggtaccgct gatttccttg ccgatttcgt cgacgatgag
    16861 gacgtcgatg tggggcagtt tcagccgggg catttcggct ttggccatgg tctgcaaggc
    16921 agcatcgcgt tcaaagaatt tatctctcgg gatgacttcc agggcggcga tgtcatcgct
    16981 ggggctctgg accatgccga cggagaaaac gacgtttaca tgctgcaaga aggctttcga
    17041 tacgcgttcc aggttgggcc cgaaagcgtc ataaccccgg ccgtggaagg tcgaagcgcc
    17101 tttgtgcttg ccgcagccga tgcagatcat cttcagcaga cctgattcgt gtttatattt
    17161 gaagtgtgta tgcggtttaa ttttgttgaa taagatgatg ccgtcggctt cgtaggcgtt
    17221 tttatcgcag tagaccggga agccatcgtc taaggtagcg acttcgacga cgtccatggt
    17281 ggaaaggacg gggacgccta agtagtcttc attgatgccg aactgagcta aatgggcctt
    17341 ttggccttca gcggtggctc cggcatggct gcccatggcc gggaagacga agggtttggc
    17401 gccccaggcc ttgagctggt cgcaaagggc tttcatgatt tccttgtagt gcgggagccc
    17461 gcggctgccg gctgtgatgc cgatgcgttt accaggcagg ctggccgtcg tcttttcctc
    17521 gagagctagg agctgttgtt tcaatgtgcc cacaggatcg gctaaatctt tatgggggaa
    17581 agcctggtgg actttgacca tctgcgggat gacgataccg tcggtcaggg attgcttgtc
    17641 gagtttggga aagaacaaag aaaggacctc ctttaaagtg gactcgcagg atagtctgcg
    17701 ggataccctt attatacgcc gttaggggaa aagaagaaat atgcaaaaaa agccctcgta
    17761 ccaaaaaaat gtattgtgta caaggacttt ttttgaggat tttgtcgaaa ttttaatgaa
    17821 attctaatgt aagcggcata ttccttactt gctttcggca tcttccggat tgaacttggt
    17881 aatcaagatg tggcggacga agatggcgat catggcgatg aagcagagca ggccgaggat
    17941 atcagctgta gactggaagt ctttgccgac cagggccagc gggttttcgc tgccgccgga
    18001 agtaacggaa acgacgatga gcaaggcgat gatgacgccc atgaggcttt cgccgacgat
    18061 gaggcccgat gcaaagagga caccgcgatg attgcaggct tctacgtctt cttcttcgtg
    18121 gccagggctg cggcgggcag cgcgggcttt cagggactta tgaatgagat aacccatgac
    18181 cgaaccgatg accagaggga tttccagtgt cggcggcagg taaatgccca tgccgacagc
    18241 cagcggcggc aatgccaggg atttcgtgtt tttcgtcaaa atgaggtcga tgatgatggc
    18301 ggcgataccg acaccgacac cgaagaggat gtagttccag tcgaggctgg cactgaagat
    18361 accttgggcg atggtcgtca tcaaggttgc ctgcggagct gccagggcct gggtcggatc
    18421 catgccggca cggggaaggg caccggtaaa gccataggcc tgatagagca ggttcaggac
    18481 cggagcgatg gcgaaggagc cgatgatcga gcccaagatg agggcgacct gctgtttcca
    18541 cggcgtagcg ccgacgaggt agcctgtctt gaggtcctgc aggttatcgt tggaaataga
    18601 agcgatgctg acgatgacgc tggtgatgaa gatagcaaag gccgtagcga actgcgtgcc
    18661 ctgcatggtt tcaaagacac cggccgaaga gccgataccg aggacgacca gggacgatac
    18721 gatgatgccc aggatgccga taccggaaat cgggctggcc gacgtgccga tgaggcctgc
    18781 catgtaaccg caggcagcag cgacgaagaa gcccatgagg atggcgacca ggatgccgac
    18841 gacgatgaac atgagcgtta ccgacatgga aaggccggcg ttgctgacga aggtgtagaa
    18901 agtgatgaac aagccgataa ggatggccag gaagaccagg ccgaccgatt tcggcgacat
    18961 gtcgatgtcc gtatgatgga gcatcttctt ttcttccgta tcgttggcgc gcaatgcgtt
    19021 gatggacatc ttgatgccgt cgacgaccgg tttggccagg cggatcaagg tccagacagc
    19081 ggcgataccg atgcagccgg caccgatgaa gcggactttc tgagcccaga tggccttagc
    19141 gaaagcggcg gcactctggc cttcagccgg cgtgatgacc gacgtcagat agggaacgaa
    19201 gacgtaccag gccaggagcg tgccgactaa gatagccatg ccgctggcga tgccgatgag
    19261 gtaacccgcg ccgaggaggg ctgtcgaaaa gcccagggga atctgggtga cggctttgcc
    19321 gaacgagagc cagtagctga attcggaaga aacgacgtgg aagccgttgg aacagagact
    19381 gaagagacca gccagggccg tgccgccgac gatatctttc atgcctgttt cttttttgct
    19441 tgtgtcttcg ccgttggctt cttccttgcg gatttcactg ccgactttca ggatttcagc
    19501 agcagcccgg ccttccggat acggcaggtc gctgttgacg accatagccc ggcgcagggg
    19561 gatagtgaac aagaccccga gagagccgcc gcaggcgcag agcagcaggg tctgccagaa
    19621 cgggaagccc tgccagtacc ccagcatgag caagcccggc aggatgaaga tgattgcaga
    19681 taaagtgccg gcagcagatg cctgcgtctg taccatgtta ttttccagga tgttggaatg
    19741 tttgaacatg cgcaggattg ccatggagat gactgcagcc gggatggacg acgagaacgt
    19801 caggccgact tttaagccca ggtatacgtt ggaagccgta aagataacgg tgatcagggc
    19861 gccgaggatc ataccgcggg ccgtcaattc cggcatactt gggtagttca cagtcagatt
    19921 tgattgtggt ttcatagaaa aacctcccaa ataaaaatta agcagtacta aaacgtacaa
    19981 cttcactgac gcttatatta tactcaattt ttttgcattc gtcatatatc aatttgtaca
    20041 ctgtacgcgg aaaaacagct gtataattac tttttcgcaa gcaaaaatat acacatgcgg
    20101 atagtagccc gatgcgcctc gtccgtggta taataaataa acgattaagc aagtcaatcg
    20161 cagagacttt ttgaagggag cgtttgtcat atgaacgatc atgaaatcat tgaaggtgtc
    20221 atccacgaat ttgcaggact ggccaagcat ccgcgtccgt cccaccatga aaaaggcgtc
    20281 agcgattata tcgtcagccg tctccatgaa ctgggcgttg ccgatgtaac acaggacgaa
    20341 gtatataatg tcatcgccaa tgttccggcc agcaagggct gcgaagattg gccgcttacg
    20401 gtcctccagg gccatatgga tatggtctgc gtcgccaagc ccggcgtcga tttcgacccc
    20461 ttgaccagtg aaatcaaact ggtccgcgaa ggcaacatcc tccacgccga tgggaccagc
    20521 ctcggtgccg atgacggcat tgccgtcgcc ttggcgctgt atctcatcca gcaggatatc
    20581 cagcacggcc cgctgcgcct catctttacg gtcgatgaag aaacgggcat gaccggggct
    20641 acccatctgg atccgaaata cgtccaggat gtgcagtaca tcatcaactg tgactctgaa
    20701 tacatggatg tcctctgtgt cggctcggcc ggcagcgtcc atacgcattt cagccggccc
    20761 atccactggc aggctgctga aggtaaacag gcgtttacta tcacagccaa agactttgca
    20821 ggcggccatt ccggtgaaac catcaacgac ggcaagagca acgccatcaa ggccctgagc
    20881 ctggccctcc gccgcattgc ccaggccggc gtatcctatg tcctggcatc tatcacgggt
    20941 ggcgtagctg ccaatgctat cccgtcggaa gcctcggctg tcatcgttgt cgacgatgta
    21001 aacgcaggtg aaacggtgaa gaaagccgtc ggcgaagaac aggccgaaat cgcggaagtg
    21061 tatggtgaag tcgaaaagaa cgcccacttc ctcgtcgaaa gtgccgacgt accggcccag
    21121 accttctcgg ctgacgatac gaagaatctg gtcagcctgc tgaacatcct tcactgcggc
    21181 gtcttcgcca tgaaccagct gctgccgaag ctgccggacc tgtcggccaa catcggtacg
    21241 atccggactg aaggcgacca tgtcgccatc cagtatttcc cccgcgcttc ggccgatgcc
    21301 cgcctgcgcg aattgatggc tatcctcccc gtttacgcag agttgacagg ctgtgacatc
    21361 gaactggctt cgccggaacc ggcctggacg gaaaatccgc acagtaaact cgttcccctc
    21421 gtcgctgacg tttacaagga agaagccggc aaagaagccc gcatcgaagc catgcacggc
    21481 ggcctggaaa cagggtactt cttctccatg aacccgaaac tggacatcat ctccgtcggc
    21541 ccgacgacgc atgacatcca cagcgccgat gaatcggtcg aactcgatac ggtagccctc
    21601 ctgacccgca tcgtcgccaa gacattggga aaattaaata attagtggct agtggatagt
    21661 gattcgtggc tcgtaggggc ttgcatgtgg caagcccgtt cctggatata tgtgatttcc
    21721 cagtcaggtg tcatgggcgt catataaata cccagcgggt cgcccacgtg gcgaccccta
    21781 cagtaaaact taacaaaaag aggctgtctt catgcgacag cctcttttgt tgtatttgca
    21841 acggttttat ttggatattt ttgctatagt ataagtgtac gaaaaggggg gagtatgatg
    21901 gaaaaaacgc atattgaccg actggttaca cccgattatt ttaaccgcaa ggaatgtgaa
    21961 ggctgcggct gttgcatcag tatctgcccg tatgaagctt tgtcgatgca gacctgcgcc
    22021 gacggcatcc ggcggccccg cgtcgatatg actaaatgtc tgcgctgcaa ccgctgtatg
    22081 gatgcttgtg aaatttacaa gtgttactat attcctcaaa aacaatatga aactgccagg
    22141 agggctgcac tatgaaatac tcgctgaatc cacacatctt tgtctttggc aagaccatca
    22201 agaagccgaa gatttccatt tcttttttat cttacctgct tcgggggaaa cagaatatcc
    22261 tcatcgacac ggttcccgat aaagcagccg atgcctatat ccaggacatc gaaagtgtcc
    22321 tgcctgtcag ccagatcgat gccctgattc tcaaccattc cgaagaagac catagcggag
    22381 ccctgcaggc cgtcctggac cgtcatcccc agctgcccat ctactgcacg ccggccacga
    22441 aggaacggct ccagccgacg tatccggaag ctgatttccg cgtcgtccag catggcgaaa
    22501 gcctgacgtt aggcgattat accttccagt tcatccatac gccggggctc cactggccgg
    22561 ataatatggt cacctggctg gcttcggaaa aaatcctctt ctccaatgac ctctttggcc
    22621 agtatttagc cgaagaaccg ccggtcgatg ccggttattc caaggaagcc atcctcaaag
    22681 gcaccgaacc ctattttgaa cgggtctttg cccctgcctc tgaaagcgat aaagccatca
    22741 tcgccgatat cgccgctatg cctatccaga cagcagcaac cggccatggc ctcatcttgc
    22801 agagccaatg gccggctgtc cgggatttct atgtatcaca cgtgatcaaa taaatatttc
    22861 gtatcgtaac tgaggtccat gtactgcgca atcgtttcgt cgtcgaatcc gtattcttcc
    22921 gacttatggg tcaaattacc ccatttgatc tggagcttct tcttggaatt caagggttct
    22981 tcgtattcga tgcgcagttc ttgtgcttct tgggagtggt cctggacctt gacaaacagg
    23041 cctttgtcga aagtcagctc gtagactttt cggaaagcat ggggaagctg gaaaccgaag
    23101 ggaacgaagt atttatcgat gaagctgttg gcgatgagga tggacccgct gtaatccatg
    23161 acgtggttga gttcatattt caggtcccct gccggcgaat ggaaggaaac gggatggatg
    23221 ccgtcgatgg caggcggttc catgccgttg ttattggtca gcaatttatg gagggtcagc
    23281 atcttttcat cgttgaagcc gaagatcgcc gtatatccgc gcaggcaggc ttcattgtac
    23341 atgatgggat ggaggccgaa ttcctcgatg ttaaagaagt cgtccacctc ttcgatggcg
    23401 acgatactga atttatacgt ggcaaaatcg aaaccgtcaa aaaattgggc gcgcatgatg
    23461 attctcctta ttcgacgaag acaggtttgt tattttcaaa acgggcgatg cgggccagag
    23521 attcgacgcg gaagccttta tcttccaacg tcctgcggcc gctctggaaa gtcttttcga
    23581 cgacgatgcc gataccggcg acagtacagc ctgcctgggc gacgatgtcc gtcaggccga
    23641 cggcagcggc tccactggcc aggaagtcat cgatgatgag agccgattcg ccggcgtgga
    23701 gataggcttt gctgatcgtt gccgtatatt cttcttcttt cgtatacgag tagatcttgg
    23761 ccgtatactg ttcgcccttg gtgagcaggg atttcttctt gcgggcaaag acgataggca
    23821 ggttgcccat ggccagggaa gccgcatagg ctacggcgat gcccgaagcc tcgatggtga
    23881 cgatgcggtc gatgtgtacg tctttgaaac ggcgggcgaa ctcctgaccg acgtgattga
    23941 tgaggttcgt atcgatttgc tggttcaaaa agttatcaat cttcaaaatc cggttgtcga
    24001 tgaccaggcc atcctgtaaa attcgctgtt tcaattcttc cattacttcc atgtcctccc
    24061 tgtgatgaaa aatggtaaac attgtctact ataatagtac tagtctaata cgattttata
    24121 ggcaagtcaa taaaattcta agtttactta tggttttaaa acaggtatac taaagttgtc
    24181 taatgagttg tctaatgata agttatctat ctttgaggga ggtcatgaca tgcagtctat
    24241 ccgacgtttg tatgttgcca agaacggtgt ttttgctgac gaagcaaaac gattgctcac
    24301 tgatttacag gaaaacctgt tgattaaagg gttgaccgat attcacattt accaccgcta
    24361 tgacgtatcc gggctcgatg atgcggcttt tgaagcggct aaaaatatga ttttctccga
    24421 accgcctgtc gatgccgtct atgacgaatt gccggttggc aaggacgaca cggtcctggc
    24481 cgtcgaatat ctgccgggcc agtacgatca gcgcgccgat tcggccatgc agtgcctgca
    24541 gatgctgacc atgaagaacg acagcctcgt ccgcacggca caggtcctcg ttttgtccgg
    24601 cagcttatcc gctgatgacg tcgctgccat caaacattat tgcatcaacc ccgtcgaagc
    24661 ccgcgaagcc tcgctggacc ccatttcgac gctggaaatg ccctgggaaa aaccggctga
    24721 cgtccccgtc atcgacggct tcgatgccat ggatgacgcc gctttgaaag aactgtcggc
    24781 acacatgggc ctggccatga gcttcgacga tttgaagttc gtccagacct acttccggga
    24841 tgaagaaaaa cggaatccga cagtgacgga aatccgcgtc atcgacacct attggtcgga
    24901 ccactgccgt catacgacgt tcatgaccga actcacggat gtttcttttg aaaacgggac
    24961 gtttacggcg cccatccagc gcgcttatga atcatataag acgacgcggg aaaacttgaa
    25021 ccgtaagaaa ccacagaccc tcatggatat ggcgactatt gccgtcaaag aattgaaagc
    25081 tgccggtaaa ctcgataacc ttgacgaatc ggaagaaatc aacgcctgca cgatcatcat
    25141 ccctgtcgat gtcgatggcg tcgaagaaga atggctcctc ctcttcaaga atgaaaccca
    25201 taaccacccg acggaaatcg aaccgttcgg cggcgcagct acctgcctgg gcggctgtat
    25261 ccgcgacccc ttgagcggcc gttcctatgt ctatcaggcc atgcgggtca ccggtgccgg
    25321 cgacccgcgt cagtcggtca aggatacgct cgtcggcaaa ctgccccagc gtaagctgac
    25381 gacaggcgct gccaagggtt acagctctta tggtaaccag attggcctgg ctacgggcga
    25441 agtcaaagaa tattacaacc ccggcttcat tgccaagcgc atggaaatcg gtgccgtcct
    25501 cggcgcagct ccgcgcaata acgtcgtccg cgaagaaccg ctccctggcg acatcatcat
    25561 tctcctcggc ggcaagaccg gccgcgacgg ctgcggcggt gctacgggct cgtccaagaa
    25621 acataccgtc gaatccctgg aaacgtgcgg cgccgaagtc cagaagggca atgccctgac
    25681 ggaacgcaag atccagcgcc tcttccgccg ccatgaagtc acggtactca tcaaacgctg
    25741 caacgacttc ggtgccggcg gtgtctccgt cgctatcggc gaattgacag acggcctgga
    25801 catcaacctc gacaaagtcc cgaagaaata cgaagggctg gacggtacgg aactggctat
    25861 ttccgaatcg caggaacgta tggctgtcgt cgtcgctccg gaacacgtcg atgaattcat
    25921 gaaatatgcc gccgaagaaa acctggaagc gacgatcgtc gctgtcgcta cggatacaaa
    25981 acgcctggtc atgcactggc gcgaccagaa cgtcgtcaat attacccgcg ctttcctcga
    26041 taccaacggc gtcacccagc atcgcaaagc cgtcgtcgtc gctccggaag acaaagattt
    26101 cttcaaggct ccggaagtca aagacgtcgc caagacctgg ctcgatacca tgggaaccct
    26161 gaatatcgcc tcggaacagg gcctggccga acgcttcgac agcaccatcg gcgcccgcac
    26221 ggtcctcatg ccctttggcg gcaaatacca gaagacgccg gtcgaaggca tggttgccaa
    26281 gattccggtc gaacacggca atacgacgac ggccagcatc ttcacccacg gctatgaccc
    26341 ggacctggct atctggagcc ccttccacgg tgccctctac gccgtcatcc agtccattgc
    26401 caaactggta gccctcggtg gcgaccgcaa aaaagtctat ctgaccatgc aggaattctt
    26461 ccagagcctg ggaaccaatg aaaaggcctg gggccagccg gtcagcgccc tcctcggtgc
    26521 tttcgtagcc cagcaggtca tggaagtggc cgctatcggt gggaaagaca gcatgagcgg
    26581 tacctttgaa aacctcaccg ttccgccgac gctcgtttcc tttgccattg caccggaaca
    26641 cacgaaccat atcatctcgc cggaattcaa agccgctgac gacgcagtcc tcctcttcga
    26701 cctgccccgc gacgctgaag aaatgccgga cttcgatact ttcaaagccc actgcgattt
    26761 cctccatcag tctgtcctcg atggtaaaat caaagccatg catgccgtcg gtcagggcgg
    26821 tatcgccgct gccgtcgccc agatggcctt tggcaatggc attggcttcg ccgttgatgc
    26881 atcctttgct acgcaggaac tgttcaacct cagctatggc tcgatcctcg tcgaaaccga
    26941 cgctgctacg gctgctgaat gggtcaaacg ggctcacgtt tccctcgtcg gcaagacgac
    27001 ggctgctgct gatgtgaccg tcgatggcgt gacgatttct ctgaaagacc tccaggatgc
    27061 ctgggaaaaa ccgctgagcc acgtcttccc catcaagagc gaaagcggta caggtgatgc
    27121 cgaactgccg ctgttcacga cgtatggtcc gcgccgcagc gaagcctttg gtacgccgcg
    27181 ggtcttcatt cccgtctgcc cgggcacgaa ctgcgaatac gactcggccg ctgcctttga
    27241 acgggctggc gctacgacgg atgtcatggt actgcgcaac aacacgccgc aggatctggc
    27301 agaatctatc gatgaaatgg ccaaacgcat tgccaaagcc cagatcatca tgtttcctgg
    27361 cggcttcagt gccggtgacg aaccggaagg ttcaggcaag tttattgcca ccttgttccg
    27421 caacccggtc ctgtcggaag ctctggaaaa cctgctctat cagcgcgatg gcctggctct
    27481 cggcatctgc aacggcttcc aggccctgat taaactcggt cttctgcctt acggtcatgt
    27541 ccagccgttg aaagctgaca gcccgacgct gacatacaat accattggcc gccatctgtc
    27601 gcgcatggtc gaaaccaagg tcgtttccgt catgagcccg tggttcagca gctgcaaagc
    27661 cggtgacgtc cacaccgtcg ccatctccca tggcgaaggc cgttttgtcg ccacaccaga
    27721 acagattcgc gaactggctg ccaaaggcca gattgccaca cagtacgtcg acctttccgg
    27781 caaagcgact tatgacagcg aatacaaccc gaaccagtcg gtcctggcag tcgaaggcat
    27841 taccagcccc gatggccgtg tcctgggcaa gatggcgcac accgaacgct tcagcgccga
    27901 agacgtcttc cagaacatcg ctggcaacaa actccagccg atcttcaaat cgggcgtcga
    27961 atactttaaa taaaagagga gcactagtgc gggccgccct ttgggacggc ccctacgagt
    28021 cgctaactac tagctgctaa tagcttgcaa gaaaaagctg ccgaatggca gctttttctt
    28081 gcatttcccc atcacctatg atataattaa caagcttgaa ctgtgtcaag ctcctaaccc
    28141 ttctgcggga agggtcatat gtccaaaaga ggaggtgatt tcgtgaacaa gtacgaagtc
    28201 atgtttattg caaaaccgct cgaagaagca gaagtagatc cgatcgttga attggtatcc
    28261 aatcttctca ccaagaacgg ctgcactatc gaaaaagtag atcgctgggg taaacgtcat
    28321 ctcgcatacc ctgttaaaaa acaggctgat ggctactacg ttttgatcaa ctttgaaggc
    28381 gaacctgacg tcatcaaaga aatcgaccgt gtcatcaaga tccacgatac tattctgaaa
    28441 cacttgatcg tcaagatcga cgaataaccg gaggcgcaaa ggtatgaact cagtccagtt
    28501 attagggaat ttagctcgtg acccggaaat ccgttttacg aagaccggcc gggcagtcgc
    28561 tatgtttacc gttgcctgta cgcggtcgta tattccggcc ggcagttcag aaccgcggga
    28621 attaactgat tttattcctt gtgttgcttg gggtaatttg gcggaaaact gtggcaataa
    28681 cttgtccaaa ggcagccgtg tattcgtcca gggccgtatc tccgttcgtt cttacgaaac
    28741 gcaggatggt cagaaacgct atcgcacgga agttgtatgc gacttcgttg cccagacaat
    28801 gggatcagaa caccagcagg ctgctccggc accgcatgct agtgcaccgc agcccaaacc
    28861 ggctgcaccg gggaccggct ttgacagcat gggttccaac acagacgaag aaatcccatt
    28921 ctaaaaggtc agacacaccc cttctaattc ttgaaggagg aaactataca tgagaagaga
    28981 tcgctcgaga aaaccgagaa gaaaagtatg cagcttctgc gtagatcatg ctgaacagat
    29041 cgattacaaa gacgttgcaa aacttcgccg tttcacaaca gaacgcggta aaatcttgcc
    29101 gcgccgcatt tccggcgttt gcgctaaaca tcagcgtaaa ctgactgttg ccatcaaacg
    29161 cgcacgtgca atcgcactct tgccgtatac ggctgaataa taaaaaagac ttgtcgcttt
    29221 gcggcaagtc ttttttttgt aggggcgaca cgtggggcgc ccgcatcgga atatgtacga
    29281 ttttcctgtc agggtgctag gcgaatcata caaatgcaac gggccgccct ttgggacggc
    29341 ccctactacg gctaacggct aactccgtct acatagcggg cgatgatgtt gcggtcgtcg
    29401 ccgtcgtaga tgaactgctg gagtttttca aaaggcgtcc gttccaggcc ttcgatgtcg
    29461 tccatgtgga tgaccagggc gtcaaaggcg tagccttttt caaagctgcc ggtctggccg
    29521 tagaagcggc ccggtccttt ggtggccaaa tacaagcctt caccgatggt gagggctttt
    29581 tctctatcgt gcttcaggga atagagtttc gatatttcaa tggtcaaggc aatctgcttg
    29641 ttcatggccg gtgtatggcc ggcagcgacg tcgctggcaa tcgtgcaggt caggccgttt
    29701 tccaggttgc gccgcaaggg catgatgcca ctggtcaggt tggcattgga ctgggcgcag
    29761 tgtgctaagt aaacgccttt atcgcgaagt aaacgttttt cttcgtctga taaatagatg
    29821 gcatgggcca tgatagtctt ttcgggccgc aagaggccaa aggtatcata gacggccgta
    29881 taagacggca agtcgggatg gagctctttg acccaggcga cttcattccg gttttccgac
    29941 agatgggatt ggacgggcaa gtcgtaggtt tcacagaggc gacccaggcc ggtcatcagt
    30001 ttttctgtcg tcgacgggac gaagcgcggc gtcgcgatga agcggacctt gtgcaatgct
    30061 tcgcggcagc ggacggccag ctcttccgta tcggccaggg agcggtccgt atcttccagg
    30121 aggtagtccg gggcgttgcg gtccatgttt actttgccga tataggcttt gaaacccatg
    30181 gtttcggcca gttccatgag ctgccacgtc gattctttat gaatcgtcgc aaaggcggaa
    30241 aaacataggg tcccttcggc ctggaggcgt ttcaggaagg ccgtatagac gcgacgggca
    30301 aagacggggg cggcgaattt cccttcggcc ggaaaggtgt aatgttccag ccagggaagg
    30361 agttcttcgt caaagccgag gccgcggttt atgtattgcg gggcgtggat atgcaggtcg
    30421 acgaaggccg gaatgatgat atcctggttg taatcgacga ctgtttccgg gcctgtcgtt
    30481 tccggcaggt ccgtcatgaa gcccgttacg acgccgtttt cgatatgggc atattgattt
    30541 tcacggatcg tcagttcatg gcgtgacggc gtgaagagaa aatttccttt aatgaacatg
    30601 taaacgctcc ttgtctatcg cttacgcagg tacgtaaacg gataagaatg atggcaggac
    30661 ttcgatgtcc aaggggaaag ccggcccttc gtcgccgtcc atgttggtcg agagatggct
    30721 gccatccagc agttcgatgc ggacgcggtc catggtcttc aaggtcatca gttccgacgt
    30781 cagcggctgg ccggccagga attccgggat gaccttgatg aggtccagat aattgaagtc
    30841 cttgaagatg cccagccaga tttttccgtc gtcgacgcgg gcctggggcg ccaggttgcg
    30901 gaaactgccg acggaattgg tcagcatgac gacgatgagg ggcgagcggt ggactgtcga
    30961 gccctgggcc gtcgtgatct tgaacagcga cgtcttatgg gcctgcatgg ccttgatgcc
    31021 ggtcaggaaa taggccagcg ggccgaggcg cgtcttctgt tcgatggata cgtggccgac
    31081 ggcttcgggc atgatgccgg cggcaatggt attgacgaag tagcggtcgt tggccttggc
    31141 gatgtcgact ttgaccgtct tgcagtgctg cagcatggcg atggcagctt ccggatggag
    31201 ggggatgttc agggcccgtg ccagatcgtt gatggttccc agggggatga agccgaagtg
    31261 gatggcactg ttggcctgtg ccaagccgtt gatggtttca ttcagcgtcc cgtcgccgcc
    31321 catggaaaat accgaatcga agccggtcag ggctgcttcc ttggcccatt ccgtcgcgtc
    31381 gccggctttc tgcgtttccc gcaattcgac gtcgtcgaac atcgtttcca gttgttgtct
    31441 taaattatcc ttgtgatatt ttgcccgttc acgccctgct gtagggttga cgattaacat
    31501 acaccgtttc aacttgctct actccttact tgtaaattgt gataagtcct taacgtgact
    31561 gatgattact attatagcgg taaaaaagtc gttcgtgaag gtgtaaacaa aggatttctc
    31621 tgattttcgt cgaactcttt aagtaatgtt cacttgtaat tttttgaaaa ggggagattg
    31681 gaatgaattt tgatgcatct gtgtatccct atggctccca ccgggaagtg gtctatggcc
    31741 gccggggcat ggtctgcacg tctcagaccc tggctgccca ggccgggctg gatatgctga
    31801 aaaaaggcgg caacgccatc gacgctgccc tggctgcggc catgtgcctg actgtcctgg
    31861 agccgaccag caacggcctg ggcagcgacg tcttcgccct gatatggacg aaggggaaac
    31921 tctatggcat caacgggagc ggctggtccc cggcaggact gacgcgggat gaattgctga
    31981 agcggggata tgaccaggtc ccgctccggg gctgggagcc ggtcatggtc ccaggggcgc
    32041 cggcggcgtg ggccgaagtc catcgccgtt ttggccgact ggcctttgaa gaactctttg
    32101 aaccggccat tgcctatgcc cgggatggtt atgcggtcat gccgaacctg gcagctatgc
    32161 tggccgacag tatggcatcc tttgcacctt atcgggggaa gaaaatcttt cagcacttat
    32221 tcgatacttt tttccctcat ggccaggcgc ccaaagccgg tgatatcctc aaattgccgg
    32281 aactggccaa gtctctggaa atcctgcggg acagccattg ccgggccctg tacgacggtg
    32341 agctggccga tgccattgat gcctggtccc ggaaaacggg cggcctcatc cgcaaggccg
    32401 atttagcagc gtatcggccg cagtgggccg acccgatcca tacggcgtac cgtggttacg
    32461 atgtctggga aatgccgccc aacggccatg gcctggtcgt cctcatggcc ctggatattg
    32521 ccagcgggtt tacttttagt aaacgggatg atgccgaatc gattcaccgc cagatcgaag
    32581 ccatgaaact ggccttctgc gatggcaaga aatatattgc cgacccgcgt ttcatgaaga
    32641 cggctgttga cgattggctc tctccagcct atgccgaccg gcggcgggcc cttatcggtc
    32701 acgaggccct gctgccgcag ccggtcgatg taaactgtgg cggtaccgtt tacttgtgca
    32761 gtgccgacgg cgaaggcaat atggtttctt tcatccagag caactacagc ggtttcggtt
    32821 cgggcatcgc cgttccggga tacggcattt ctctcagcga caggggtcat aacttctcta
    32881 tggacccagc cagcgataac tgcgtcgggc cgcggaagaa gtcctatcat acgatcattc
    32941 ccggcttttt gacgaaagac ggccgtcctg tcggcccctt cggcgtcatg ggcgctttca
    33001 tgcagcccca ggggcagttc caggtcctgc tgaataccat cgactatcat ctcaaccctc
    33061 aggcagccct ggatgctccg cgctggcagt gggtcggcgg caagaccatt gaaatggaac
    33121 gggcctttcc aaccgctgtc attgaagaac tgcgccgccg cggccatgac gtcgtcgtca
    33181 aagacgatat ccagacctat ggccggggac agatcatctg gcgcgatgac caggggacgc
    33241 tcatgggtgg gaccgaaccc cgggccgatg gctgtgtcgc cgcctggtaa aggtcgttta
    33301 cttttcttct tccggaaaat atgataggat ggagaaaact aaaggaggac tgtccccatg
    33361 aaaccgaaac tttctatttt agaccctaat ccgacgccgc tccaatacct gtccggcctg
    33421 tcgcaggaat tattccgtga aatctatatc aagcgcgatg acctgacgcc tttcggcggc
    33481 ggtggcaata aactgcgtaa attagagtat ctgatgatcg aagccctcaa ctctcaggcg
    33541 acgaccattg tgaccgtcgg agcgccgcag accaaccatg gccgcctgac ggcggctgtt
    33601 gccgcgaaat tcggcctgaa atgcattatc gtcgctgtcg gtgatactga tggcgaactg
    33661 agcggcaatt tactcctcga cggtattttt ggcgcccgcg tcgtcctcaa gcatgacgat
    33721 ggccgcgacc acgacgtcca gctcgaagaa acagtcaaga aagtcatggc cgccgaatta
    33781 aagaagggtg aacaagttta cttcatcccc atgggcggca gcgatacgac gggtatgctg
    33841 ggctacatgg actgcgcccg cgaactcgac gcccaggcca aggaacagca g
//
LOCUS       DAWCYS010000003        29195 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_91_length_29195_cov_8.776836, whole genome shotgun sequence.
ACCESSION   DAWCYS010000003 DAWCYS010000000
VERSION     DAWCYS010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 29195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29195
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_29195_cov_8.776836"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6526 a         8031 c         7735 g         6903 t
ORIGIN      
        1 ttcttatcgg gactgcgcat gaaggtttcg ccgatgagga cggcgtcgac gccgtggtcg
       61 cgcaggacct tcgtgtggtc tgccgtcttg atgccgcttt cggcgacgaa gaggatgtcc
      121 ttcggtacca gcggccgcag gcggcagctc gtatcgaggg ataccgtgaa gtccttgagg
      181 ttgcggttat tgacaccgat gatgcgggca ccgatggcga tggcccggcg cacttcgtct
      241 tcatcgtggg cttcgaccag ggccgacagg cccagagaat cggccagctg gaagaaggtc
      301 cgcagccggt cgtcgtcgag gacggcgcag atgaggagca cagccgacgc gcccaaggcc
      361 cgggcttcgt aaatctggta ttcgtcgatg atgaaatcct tgcgcaggac cggcgtatcg
      421 acggcagcgg cgatttcctg caaatactgc gggctgccca ggaagtaatc ccgttcagtc
      481 aggacggaaa tggctgcggc gccggcctgt tcatattctt tggcaatggc gacgtaaggg
      541 aaatcgggag cgatgaggcc cttcgacggc gaggccttct tgacttcaca gatgaaagac
      601 aagcccggct tcttcaggtt cctttcaaag gggaatcctt ctttcttcgg agctgccagg
      661 gcccgctgct tcatgacttc cggcgggacc tgctgtttat ccgtgaccag ccgcttggca
      721 acggctgccg taagatcatc taatatcata gggcaccttc tcactcgtcg ctgacggcaa
      781 tgcatttttc cagggctgcc gtcgccttgc cgctgtcgat gaggctggct gcgagctgga
      841 tgccgtcggc gatggaatcg gctttgccgg cgacaaagaa ggctgcaccg ctgttcatga
      901 ggacggcatt gcgtttcgtc ccctggattt tcccggacag gacatcacgg gtaatctggg
      961 cgttgacttc cggcgtcccg ccgacgaggt cttctttcgt ccccggcgtg aggccgaagt
     1021 cttccggttt gatgaccgat gtccggtaat aaccgtcgcg gagttcacag atcgtcgtcg
     1081 ggccgttcgg cgagatttcg tcgagttttt cctgaccgta agcgacgagg ccccgtttga
     1141 cgccgaggga ggccaggacc ttggccagcg gttcgacgag atagccgtca tagacgccga
     1201 ggaggaacat ttccggcttg gccgggttgg tcagagggcc gaggatgttg aagaccgtcc
     1261 ggaagcccag ttccttgcgg atagggccga cgtatttcat agcagcgtgg tatttctggg
     1321 caaagaggaa gcagaaattc gtttcttcga gcatcttcaa ggccagctgc gggtcctgct
     1381 ggatgttgat gcccagcgct tccaggcagt ccgccgtacc gcacagggat gaagcggccc
     1441 ggttgccgtg tttggcgacc ttgacgccgc ctgcggccag gaccatggcc gacgtcgtgg
     1501 aaatattgaa agaatgggcg ccgtcaccgc cggtgccgac gatttccagg acttccatgc
     1561 ccggatgggg aacgggcgtc gccagggagc gcatggcttc ggcacagccg gaaatttcat
     1621 cgatcgtttc ggctttggtg ctcttcgtcg aaagggcagc caggaaagcg gcgttctgag
     1681 tcggcgtcgt ttcgcctttc atgatttcca tcatgacctg atgggcttcg tcgtaagtca
     1741 aatcgccttt attgacaatc ttgataatcg cttctttaat catcgtaagt acctccattc
     1801 aaagagtcat ctatattact tataatgtaa ggaaattttc taccatcatc ggcccctggg
     1861 gcgtcaaaat cgattcggga tggaactgca ggccgtagac gggatactgc ttgtgaccga
     1921 cggccatgat ttcgccgtct tccgtatggg ccaggacttc caattcttcg ggcagcgtat
     1981 ccgggtcgag ggccagggaa tgataacggc cgacgtcgac ctgttccggc agccctttaa
     2041 acaacgggct gtccgttttc agcgtcgcta cggactgttt gccgtgcatg agctgcttgg
     2101 cataggtaat ggttccgccg tagacttcgc agatggcctg atggcctaag cagacgccca
     2161 ggatggggac cttgccggcg aagtagctga tggcttcttc gcagacaccg gcatcggcgg
     2221 gacggcccgg accgggtgaa atgatgaggt gatccggatg catggcttca atatcctgga
     2281 cggtcttttc atcgttgcgg atgaccgtga tatccgggtc gaactgaccg aggagctggt
     2341 agagattata agaaaaactg tcgtaattat caatgagaac tatcatttgt cgattcctcc
     2401 attagcctgc tgtaatgcaa tgacgacggc tttggccttg ttcagacatt cctgatgttc
     2461 ctgttcgggg atgctgtcgg cgacgatgcc cgcaccggag caggcataga cgtgattgcc
     2521 tttcttgtaa gccaggcgga tgccgatgca gcagtccata ttgccggtaa agtcgatgta
     2581 gccgatggcg ctgccgtaga cgccgcgttt gtggccttct agttcgtcga tgatttcaca
     2641 ggcccggatc ttcggcgccc ccgacagggt gcccgccggc aggaccgaag caatggcatc
     2701 gacagccgtc ttgtccttgc gtatcgtact gtggacagtc gagccgatgt gcatgatgtg
     2761 ggagaagcgc aggacgtcga gatagcgttc gacgtggacg ctgccgaatt cgctgatgcg
     2821 gcccaggtca ttgcggccca ggtcgacgag catgttgtgt tcggccagtt ccttggtatt
     2881 gtgcaggagt tcgtcttcca aggcctggtc ttcttccggc gtcttgcccc gcggccgcga
     2941 gccggccaga gggaaggtaa agaggtcgcc attttccagg cgggtcagcg tttccggcga
     3001 agaaccggcc agttcgatat cgtcaccaga gaagaagaac atgtacggcg acggattgat
     3061 ggtccgcagg acgcggtacg tatcgaggag gctcccttcg gcttcggcgc tcaggcggtt
     3121 ggccaggacg acctggaaaa tatcgccttc acggatgtag ttcttgccct tttcgaccat
     3181 ctggcaatat tcttcttcat tgaacaaggg tttgaaatcg ctcttgagct gcagcggtac
     3241 gtccggtgcc ttcttgcctt cgcagatgag gctttccatg cgcttcattt ccaggagggc
     3301 ccggttgtaa ttttcttcaa ccgagtcgag gcggacattg acgatgaggt aaatctgctg
     3361 tttcaggtta tcgaagacga tgaccttgtc gaacaacatg aggtcgaaat cgttgaagcc
     3421 atcatcatcg acggcattga gcttcagcga cggttcggcg tatttgatga aatcataggc
     3481 aaagtagccg accaggccgc ccgtaaaggg aggcaggtcc gaaaggcgcg gcgccttatt
     3541 atctgccagg atctgggaaa tgagctgttc cggatggtcc gtatggatct ggacttcggc
     3601 gccggtcttg atgtggaccg tgctgtcctt gcagtacagg cccatctgcg ggtcatagcc
     3661 gatgaaagtg tagcggcccc agcgctggga gtcttccatg ctttccagca aatagcaatg
     3721 agagctgatg ttcttgacga tgcgcaatac ttcgatgggc gtgcggacgt cggcatatat
     3781 gctgcggcag atgggcacga ccttatattc accttgatat gcttttacct gttccagact
     3841 gagcgtttcc atatgagcct cctaaaaaaa gaatccgtcc atgacaatgt tacttgtcaa
     3901 ggacggatta agttccgcgg tgccaccttg tttcagggat cgagccctac tctcgacgga
     3961 ataccaacat attcccggca actgacgtat gccaacacgt cgcagaatac tcagccttac
     4021 ggcctttgac tgcgccctca gcggtccatt taacgagctg cgtcggccgg ctctcagcac
     4081 cccggctctc tgtacgtgca cgtcatcgtt tttacttccg cctcaaaggt ttcatggacg
     4141 atgttcattt gatggttgta tgatacaccg cattttttaa cctgtcaagg agatttttgt
     4201 ccatattcta aagacaatcg ttttactggc aaaaaagagg ctgtcgcatg aagacagcct
     4261 ctttttgagt tttaagtttg tagtttttag ttttcagcaa atggtaaaaa atttaaaacc
     4321 atcttctaca aactacacac tctgtactac aaactttacg atcgaatgag tcactcctga
     4381 tgacagagtc ctttacgcac gccctacatc atagcgatga ccggcgagat gatgagcagg
     4441 atgccggcga agacgatgag gaccagggag atgcctttgt atttatgcag ggcatcgact
     4501 ttgtaaacca ggtatgctgg gatgaggcag gcgatgatac cgaagatcgg gccgagcagg
     4561 gtcaaaagct tgaggaccgg tgcattgagg acgatggcgc cccaggcgac ggcgacggcg
     4621 aagatgagga tgccgtgacg gacgacggtc ttgctgattt tttcttccgg aatgaagcgg
     4681 cgcaggatgt tcatagcaat cccctggcag gcttcacgga agccgaggaa gacgctgaag
     4741 aaagccgtca tgacagcgaa gatgttgagg ataaggctga agatcttgac ggcgctgcca
     4801 tcaaaccctc tggcaacgat ggccagggcc gaaatgttct gggagtaagc gaggacggcc
     4861 tggtcatgac cgagggccag gttgaaggac atagcgtacg agaaaatcgt gatgaacaag
     4921 acggagaaag cgattttcat agcccgcatg gctttataac gggcgacgtg gatggacttg
     4981 ttgtgagagc ggtaggaaat gaccatcggg ctgatgctgg ggatgaagag gatcgatgtc
     5041 agcgtgaccg gcagcatggc gatggtattc ttgacgaggt agcccaggtc cgggaagacg
     5101 ccgatgttgg ccaggttcca atgctggatc atgatgccgc ccaagcagat gacgactaac
     5161 aatttcgtca ggaccatgag cgtcgatacg cggaacagga gctgttcacc ccgggaggcg
     5221 atgaggacca tgacgcagat gatggccaat ccgtagaagg ggctatccga caggagcgtg
     5281 tccgttacgc cgaaggaaaa caggaaggac gcgctgtcgt tggtcagggc tgtcgaatac
     5341 atgaagacgc agatgaggat gctcaggaag tagaggatcc ccaggaggaa gccccagttc
     5401 ttaccgaggt aaccgctgat gacgctggga taatcttcgc accgcggcga atctgccagg
     5461 gtattgatga ataagcgctg taagagatag atagccggat aggcgatgat agctgagaat
     5521 aaataaaccc atacgccgac caggcctacc tgaacgggca aaaagacgat accggcgccg
     5581 atggccatgc cgatactcat aataatccag ccccagtccg tactgtcgaa cttgacggct
     5641 tctttccatt gtgcatctgt catgcccgcc tgccgggcca atgctgctgt tgagttcata
     5701 tgaattacct cctatgatgg atacatttga aaataaagtg tctctcttaa cattacatgt
     5761 ttgtcgatgt catcggctcc cttttctcaa tttctgttca tatacatttt atctatatca
     5821 gttctggtct attttccaaa aaccgtaaaa tttaatgtat atgtcggcgt ttcacctttt
     5881 actaaaacgt tctatttctt acttaaagta taacatggaa ggtatatgct gtaaaatatt
     5941 cattgcgcaa tttactttta taggaaattc ctatatcaac gacttatttc ctttaaattt
     6001 tcttcatcat ttgctaggtt tttcatatat tgagaatgac ttgttctcac gattccagca
     6061 tttctatgaa gaaatgtcct ttccaaaaag tcaaatttca atatattttc tttataattt
     6121 gtaagtcatg gctagacatc tcttttcaac tttttgctcc tatttattct ttattttcat
     6181 tttaaatatt tctttattgt atcaaaaact cataactttc ctctattttt ttattttgat
     6241 acaataaaag tacgatatgc gattctatag gtacggttga tattcgaggt gttctattat
     6301 gactttacag caacttcgtt atatcttaga gttatctaag cacaattcaa tttcggctgc
     6361 cgcacaggct ctgaacatct cccagcccag cctgagcacg gccatacggg acctggaaaa
     6421 agagttctcc ctgaccctgg tcgagcgcaa ccgccatggc atcacgttca caccggcagg
     6481 gctggaattc ctgcactatg ccggtcacat cctctcccat gcagcggcca tgcacgacca
     6541 tttccaggca gaaaaaagac aggacctgcc ccagatcctg tccatctctt cccagcacta
     6601 tccctttgcc gatgaagcct tcatccatgc cctgcaacac gtccagccgg gcaagtcctg
     6661 cgtattcacc ctgcgcgaag ggcggacgtc gcaggtcatc cacgacgtcg tcacccgcga
     6721 gagccatctg ggcatcctct tcttgtccca tatgaacgag cgcttcctgc aggacttatt
     6781 ccacaaaaac aagctggaat tcacgtctct ttgcgccttt acgccctgtc tttacgtccg
     6841 cagcggccat cctctggcag cgtgtcagca cgtcgttccc agtcaatttg cggaatatcc
     6901 ctgtgtccgc tttgaacagg gccatgacaa gtcccagttc tcggaagaac tgctcctgcc
     6961 caatccggcc agccgccagt gcatctacgt caccgaccgt tccaccctgt tcagcgtcat
     7021 acggtctacc gatgcctata ccatcggcac gggccatgta gccccgtcga tcatcggccg
     7081 ccatatccag gccattccca tcgaaaatcc cctggatacg atgactgtag gctggatcaa
     7141 gattgccgga gaacccctgc ccgaagaagc ccgccactac atcaccatcc tggccggcct
     7201 cttagaagga cgggagcatg gctagtggct cgtagttcgt ggcttgtaat ggctgtctca
     7261 cgaagggact cataatccgt aggggctcgc acgtggcgag cccgtcccag gatacatgtg
     7321 attttctagc cggtctgcta ggagaagctt ctagattcct aacgggtcgc ccacgcggcg
     7381 acccctacag tgcaaaaaaa ccgccccatt aagcgtgttc ttgcttaatg gggcggctcc
     7441 ttttttcact tacaacgaac ttatttattt gtcatcatag gcttttgtca tgcggacata
     7501 gccttcgtag cggtccttag catcttcttc ggtcttttca aagagtgctt ctgccgtatc
     7561 cgggaaggtg cgctgcaagg acgagaaacg tgtttcaccc atgaggaagt cgcggaagtt
     7621 ggctgttggc ggttttgaat cgaggatgaa ggggttcttg ccctgtttct tcaattccgg
     7681 attatagcgc cacagatgcc agtagccggc ttcgactgcc cgtttttctt cgagctggct
     7741 ctgaccctgg ccgactttca ggccgtggtt gatgcacggt gcataaccga tgatcaggga
     7801 cgggccgttg taggcttcgg cttctttgag agcctttatg aactggttct tatcggcgcc
     7861 catagcgacc tgagcgacgt agatgtagcc gtactgtgct tccatcatgc ccaagtcctt
     7921 cttgcgcgtc ttcttaccgg aagctgcgaa ctgggcaaca gctgccgtcg gcgtagcttt
     7981 ggaagcctgg ccgcctgtat tggagtaaac ttccgtatcg aagaccagga cgttgacgtc
     8041 ttcgccctga gccaagacgt ggtccaagcc accataaccg atatcgtaag cccagccgtc
     8101 gccgccgaag atccactggc tgcgtttgac caggaactgt ttcatatcat agacctgttc
     8161 cagtgccggt ttgccgtctt tttcagcttc caggagggca atgaccttat ccgaacgttc
     8221 gcgggtgcct tcgctttcag ccttgtgttc tttccattcc gtaaaggctg cctgcagttc
     8281 cgggctggcg acgtccatag cggcatcgat gtactgttcc agcagtttcc gttccgtctt
     8341 ttcagccagc atcataccga aaccgtattc tgcgttgtct tcaaagaggg atgtcgacca
     8401 agccgggccg tgaccctgag cgttcttggt atagccacag tccggtgcac tgccacccca
     8461 gacgctggag cagccgtgag cgttggcaat catcatgcgg tcgccgaaga gctgggtgac
     8521 gactttacaa tacggcgttt cagcacagcc tgcgcaggcg ccggtgaatt cgaggagcgg
     8581 ctgttcgaac tggctgccga tgacggtatt cttatccatc gggttggctt tgaccggcag
     8641 gccgatgacg taatcccaaa gagccgcttt atattcttcg ccgtcgaaag gtttcatggt
     8701 caatgcctgt ttcgggcaga catgggtaca gctgccgcag cccaggcaat ccatgacgga
     8761 tacgacgata ttgtacttgt agcccggagc cactttgatt tccttgtcct ggtagccttt
     8821 cggagcctta gcggcttcat caggcgtcat caggaacgga cggatagcag catgggggca
     8881 gacgaaggaa cactggttac agccgataca tttttcagca tcccagctcg gtacgaacat
     8941 ggcgacgccg cgtttttctt cggcgcaggt ccccgtcggc caacggccgt cttcgatgcc
     9001 atcataagcg ctgaccggca ggtcgtcgcc ttcaagggca ttgacaggaa tggagaattc
     9061 gcggaagaat ttcgtatgtt tcgacatgtc gaccggttca tcgacagcat cagcccaggc
     9121 ggccggtaca tcgaccttga cgatagcaga caggccctgg tcgatagctt cattgttcat
     9181 gtcgacgata ttctggcctt tcttgccata cgatgcgacg acggattctt tcagtttcgc
     9241 aatagccagg tccagaggaa tgacatcggc cagtttgaag aaggccgact gcatgatcat
     9301 gttgatgcgg ccgccgagac cgattttctg agcaatgccg acagcgtcga tgatgtagaa
     9361 tttgacgtcg ttgcgggcaa tagcgcgttt taatttagcc ggcagttttt catccagttc
     9421 ttccggtttc caaaccgtat tgagcaggaa cgtaccgcct ttcttgatgc cccgcaggag
     9481 gtcgaagcca tggacatagg actggcggtg gcaggcgatg aagtcggcat tattgatgag
     9541 atacggcatc ttgatcggcg tatcgccaaa gcgcagatgc gatacggtga cgccgccgga
     9601 tttcttggag tcataagcaa aataagcctg agcatacata tcggtgttat cgccgatgat
     9661 cttgatggca ctcttattgg cgccgactgt accgtcggaa ccgaagcccc agaatttgca
     9721 ggctttcagg cctttgccac ctgtcgtgat gcctttgacg cgcggcagag atttataggt
     9781 aacatcgtcg acgatgctca aagtgaagtc gttcttcggt tcgaaggcct tgaggttttc
     9841 aaaggcagcg acgacgtcgc ccggcaagaa ttctttcgat gcgataccat agcggccgtg
     9901 gtagactttc ggcgcattgt ctgctacggc aaaggtagca gctacgctgt gatagagcgg
     9961 ttcgccgttg gcgccggttt ccttggtgcg gtccaggaca gcgatcttct tgaccgtatt
    10021 cggcagagcg gccaggaaat gcttttccga ccagggccgg aacatgtgga cgttgataca
    10081 gccgaccttt tcgcctttat cgacgagcat cttgaccgtt tcttcaatgg tctgggcgcc
    10141 ggaacccatg gcaacgagga cgaattcagc atcaggagcg ccggtatagt tgaacaggtg
    10201 gtattcacgg cccgtgattt cactgatttt ctgcatgtaa ccttcagcga catcgggaac
    10261 gcggtcgtag aatttattga gggcttcgcg gtgctgcata aacgtttcag ccggttcgca
    10321 gaaagaaatg gcttccggat tatccggatt catgccgcgt ttgcggaagg cccggacggc
    10381 atccatatcg aggagaggcc ggagtttttc atacggcagg acttcgattt tctggatttc
    10441 gtgggacgtg cggaagccat cgaagaagtt gatgatcggg atgcggcagg aaatagccgt
    10501 ggcatggacg acagctgcca ggtccatgat ttcctggaca ctcgattcgc acatcatgct
    10561 gatgcccgtg ccgcgcatag ccatagcatc gtcatggccg gcaaaaatcg tatagccgtt
    10621 ggtggcaact gtacgggatg cgacgtggaa gacaccgggc aggagttcgc tggaaatctt
    10681 gaacatgttg ctgagcatga tcatcatgcc ctgggaagat gtaaaggtcg tcgtcaatgc
    10741 gccggtattc aaagcgccgt gcatagcacc ggcagcccct ttttcagact gcatttcctg
    10801 aacctggacg gtcgtgccaa acaagttctt acggccatga gcagcccatt catcgacgtg
    10861 ttcagccatc ggcgacgacg gcgttatcgg gaagatggaa gcctgttccg taaaggcgta
    10921 ggcaatataa gccgcagctt cgttgccatc cattgtcttg aagattttct ttgccatagt
    10981 acttttcccc tttctgtaaa acctggtaac tgaaagaatc cgtgtacact taggagtcat
    11041 ctttaacatt gcgcagataa aatcatcttt tttcactata tgactaaggt attttttaga
    11101 tgactatttg ttatcttcat tgtactattt cgaaaaaatt tatgcaaggc ctttctagta
    11161 aaacgttcat cttatgccga ttttgtctaa acgcgtatat attcatgaaa aagagtcata
    11221 aaaaaaacag gctgtcacac aaagacagcc tgttttttag tccttagttt gtagttttca
    11281 gtttttagtg aatggacagc taaaaattta acctgattct gcaagaagtc tcccgcctct
    11341 ataggcggga gtacgttcac aaacctctac gcctgctaca aactctgtac gataaactat
    11401 attacgccca gatggatttc atgagctctt tgtacgattc gccgaggtgg cggatgatga
    11461 cgtcgaacat ggcgtaaatc aaatcgccat caacttcatc gtctttgaga aggacgcacg
    11521 tatcgaggat gaggttgcct tcatcatcga catagtattt gaaggacttg tactgcttgt
    11581 tgtacgtatt gagcagcttg aggacggcgg cttcattgtc atcgtgcagg accttcggcg
    11641 ctaccaggac gcggatcatg ccgtaaatgc tggtgtcgag gatgacgatg agggacaggc
    11701 ggttgccatt gatgtcagca gccgaccgga agacgaccgt ctggaactgg tcatccttga
    11761 tttcatcgac cgtaaaagcc ttgatatcct tttcgtccag atatttcttg aaattttccg
    11821 cttttttatt catgggaaac actctccttt ttctacaaat aacacttcgg ttttattata
    11881 ccccatttca tcagggcttg tcatataaaa aagcgctgtc gcgtgaagac agcgcctttt
    11941 tgagtattat gtttgcagtt tgtagttaag ttttttaaat ttaaagctta tactgtaccc
    12001 ataaggttgg acagtcccct ttttaagtgg ttagcagcta gtggttcgtg attagcagat
    12061 ggttttatag tttgtaatac ggatcttgta gaggcgccac gtgagcggcc cgttagggat
    12121 tgggataatt cttccagcaa acggggcggt aaatcataca tatcccgaag cgggcgcccc
    12181 acgtggcgcc cctacagaaa agggactgcc acatgtgcga cagtcccttt tcttattagg
    12241 agaggagtag tttttcgagg attgggtcta attcttccgg tgtcgtgctg ggttcaaagc
    12301 gctggacctg gctgccgtcc cggttgatga gaaatttggt gaaattccat ttgatgccgt
    12361 tgccctgggc attgtccgga tagtattcgg aaacgacctt ctggatgagc tggccgcttt
    12421 ctttatcggt ttcgtagcct ttaaaaggcg cggctcccgt gaggtattga taaagcggag
    12481 ctgcctgatc accgcgaacg tcgattttcg caaagagcgg gaaggtgacg ccatggttga
    12541 ggcggcagaa ctgttcgatg tgctggttat cttccgggtc ctgttccagg aactggttgc
    12601 acgggaaacc gaggatgacc aggcctttat ccttatattt ctgatataag gcttccaggc
    12661 cgtcgtactg tggtgtaaag ccgcatttgc tggccgtatt gacgatgagc aggactttat
    12721 ctttatattg gctcagggat acatcgtgac cctggatatc tttgacagtg aaatcgtata
    12781 cgttcatgac gtgacctctt tatggtttat aattcgtgga taacgcagcc gccaaattcc
    12841 ggcgagcagg tggccccgtc gagggacggc gcttctttga cagcgaacga ctccttccac
    12901 aggccgtgga ggttgcagta tgcatagact tcgccggcat cgcctgtttc aaattcagcc
    12961 ttcggttctt cacctggttt gaggtaacgg atgtcgacgc cgttgtcatc gacaagggca
    13021 atccattcga tgtaatgctt ttcttccatc gggtgtgtca ctgagccgac gtggacttcc
    13081 agatgaccat cgattttctg gatagccggt acgtgttttt ctttagctgc atcgacagtg
    13141 tcggcgacga gatgagtcaa ttcctggccg gcatagatga gtttttccgg tacttgcgtg
    13201 cctacagata ctaaaacgct gccatcttta tcgttacggt aaaattctac gtgtttcata
    13261 tgggttcctc cttcggggct tggcccctta ataattattt ttctttatgt gctcattata
    13321 gactaaatcg gtacgcttgt caataatgga gaataattat tctttattat tttttcttga
    13381 tatccagctc aatgctgtgc gggtctttct ttagggcctt catccgttcc cgcgtcgtcc
    13441 ggctggcacc gccccatttg aggaccagtc caccgtttac actccacgtc gacgtcatat
    13501 tggagccgga ccgccttgtc aattcgcccc agacggaatt ccccttcccc aaagacaggg
    13561 acgtcccaaa agtcatgtca taccaggtgt aatggctgga accgacatcg gccgtctggg
    13621 gtgcctggcc atcgccatgg agactgcccg tcgtagtccc attcatttcc cgctgaacgc
    13681 caaagcggcc atagagcagg ccggcatggc cgttgacgct atacgatttg ccggccataa
    13741 tgcccaggcg tccggtgaca tagggtttat tcccggaccg ataggtcacg tcgtctccgc
    13801 tgaccgtgta agggaagaca taactcaggg acaggccggc ctggggttcc caaaagaatt
    13861 gcttgggcaa gagacgcgtt tcataatagc gtataccagc agcgatcagc cagttccggt
    13921 agttgaccaa atcgctattc ccatcggcat cgctataaga gacagtatgc tgcaactggg
    13981 acaggcggat gacggcatca ccgtgacggt cgtgcttatc gccccattcc atggcaaaac
    14041 cgccgccata ttccttgata gagtcagacc cgccaccata gggctggctt cctgcctgac
    14101 cattagtata tacattcgac gtgctcatcg tataaaaagc gccctgatgc agggtcccgc
    14161 cccaacgggc catggcatgc ggcgaatcat aaccgatgag ggtcgcactg tagtcctggc
    14221 tgaaagacag gctgccgtaa tcgggaaagg tatagctgcg ataatcggtc ttgacccacc
    14281 agcccgtatc ttctgatggt tcggccaagt ctttataaga agcgaaacga ttatcatcgc
    14341 tgcgccgcca gaaaaggtcc gacgccattt cataaaaaga agccgtccca gcctgtgtcg
    14401 ctgccaaaga ccggccggca gacgaccagt ccgccgttcc tgcagcagct gctccggccg
    14461 gacagaccag gagcgaaaaa gcgattatcc aattacgcaa accaggcaga ctgaatttca
    14521 taaaaacctc cttcgaggac ttatgtttcc gtccttacta aatcataatg gcggcagccc
    14581 tgttcttcac ggcagcacag gacaccgtgg tcgacgagat attcaatatg ggccagcgtt
    14641 tcccccaggg cgaaccattt ctgcgtcggc ggaaagtcct tccactgctt gccgcgcatg
    14701 gaccaggtca tatgtggggc gatgtcgtag gccgtgctgt gcggatagag ccctgccaga
    14761 tggcagattt ccgccaggcg tcggtcgtga tgggccacga tttcgtcgat gcggccggca
    14821 atggacgtat cgccttcacg gtgtcccggc agagccagtc ggacggggag ttcccgcact
    14881 ttctgtaaac tttcgagata gcgttgcagg gcctgtttca tatgatacca gacctggatg
    14941 ttcggcgtaa tgtcgaagag gatgtggtca ctggtaaaga agacttcttc tttcggcagg
    15001 tacaggcagc acaggcccgg cgtatggccc ggcgtgtgaa tgacctgtaa atcgcagccg
    15061 gccaggtgga ggcagtcgcc gtcatggaca gggttcaccg ggaaatgcgg gtctggcgaa
    15121 aaggtccggg cctgattgga gaactgcgtc ttgatttcgg ccggcggcat gccttcgcgc
    15181 atgaagttat cttcagccgc tttccacgtc gaaccgtctt cggaatcgac gaggtactgg
    15241 taatcgacgg gatgcatgta aataggacag ccggcctggg caaagaggcc gacgagaccg
    15301 ctgtggtcag cgtggagatg agtgatgaac agggacgtat cagattgtaa acggatgtcc
    15361 agttcttgta aaccctggca caaggcgcgt ttacattcag gacggttaaa gcccgtgtcg
    15421 atgactagat tgccagacac tcctttgatg acataggcgt tgagatactg caggggattg
    15481 tcaggcaaat cgacgcggat acggtatata tcgggatgag aatgaacttg ttggatcatg
    15541 ggacctcctt ttaaaggaat caggcgggcc gccctctggg acggccctac gaataaaggc
    15601 ctttaaattt aaaaccattc actaaaaact acagactgca aacttactac tcaaaaagag
    15661 gctgtcagat gacagcctct tttctcattt cttcttttta ggattatagc ggttcatgcc
    15721 tttgtcgacg cccaggctca ggcagccttc gatagctgtc gctgcgtctt caatgccctg
    15781 ctggtaggct tcacggagtt cttccggaaa aggagtcagg acgtggtcga cgacgtcatg
    15841 gtgcggcaag ggccggccga tgccgacgcg gaaacgggtg aaatcggtac agccgttagc
    15901 aaacaggctc ttgatgccgt tatgtccgcc atcgctgccg ctcttgcgga tgcgcagacg
    15961 gccgacggcc aagtccatat cgtcataggc gacgtagaca tcgggctgat cgattttata
    16021 ccagcgcatg agcgggccga cggcgcggcc gctttcattc atgtacgtct gcggcttgac
    16081 caggatgatc ttttcgccgt tcaccgtggt ctgggccacg agggcgtcca tcttatcctg
    16141 ccacaaggtc acgttccagc gcttggctaa ttcatcgacg acacggaaac ccgtgttgtg
    16201 tttcgattct tcatattggc ggcccggatt gcccaggcct acgatcatat gcacgacagg
    16261 tcccttattt gtcgaagaga cggcttacgg aaacgtcgtg gaagatgcgc atgatagctt
    16321 cgccgaggag cggtgcgacc gaaaggactt tgatcttatc gatctgtttt tcctgcggca
    16381 aaggaatggt attggtgatg atcagttcgg aaatgactga tttctggatg cgttcgacag
    16441 ccgggtcggt gaggacagcg tgagcgcagg cagcgaagac acgggaagca cccatttctt
    16501 tcagggcttt cgcgccaccg cacaaggaac cagccgtatc aacgatatcg tcgacgagga
    16561 tgcaggtctt gcctttgatg tcgccgatga tgttcatgac ttcagcaacg cccggacggg
    16621 gacggcgttt ttcgatgatg gcgataggtg cattgacgcg gtcagccaga tcgcgggcac
    16681 gcgttacgcc accgaggtca ggagatacga cgacgatgtc gcccatatcc gtttcttctt
    16741 ttttctgatt gatgtatttg gcaatgatgg aagccgagcc gaggtgatcg acagggatgt
    16801 cgaagaagcc ctggatctgg ccggcatgca agtccatggt aacgacgcgg gtaatgccgg
    16861 ctgtcgtcat caaatcagca accagtttgg atgtaatcgg ttcacggccg cgggtcttgc
    16921 ggtcctgacg tgcataacca taatacggaa caacggcggt aatgtgttta gccgaagcgc
    16981 gtttgagggc atctgccatg atgagcagtt ccatcagggt gtcattggcc ggctgacaag
    17041 tcggctggat gacgaatacg tccttaccgc ggacactttc gtcgatcatg acctggattt
    17101 caccgttgtt gaatttgcca acaaaggctt tgcccagttc caaaccgagg taatcggcaa
    17161 tttccctggc caattccgga ttggcattcc ccgtaaaaat cttcagcttt ttcccgtctt
    17221 cgtaactcat agtgagcctc cctgtataag tagtgattaa ttgttatttt ttatatgtat
    17281 cttccttgac ccagccttca atgttgcgct gccgtgcccg ggcaacggat aaggctttgg
    17341 ccggaatatc tttggtaatc gtcgaaccgg cgccgacata ggcatagtcg ccgactttga
    17401 ccggtgcgac gaggttgctg ttgcagccta cgaaggcatg gtcgccaatg atcgtgcggt
    17461 gtttgatctt gccgtcgtaa ttgacagtga tcgtcccgca gccgatattg acaccactgc
    17521 cgacatcgct gtcgccgatg tagctgaggt gcggcagttt cgtgccatcg ccgacggtgg
    17581 agttcttgac ttccatatag ttgccgactt tgacctgatt gccgatgacc gtattgggcc
    17641 ggaaatggac gaacgggccg atttcacagc cgttcttgat ttcgcagtca tgagcataca
    17701 tgaactggac atggtcgtca tcgcccatct tgacgttagt catgcggacg tacgggccga
    17761 cctggcagcc ttcgccgatg accgtatcgc cttcgaggat cgtccccgga taaaggacgg
    17821 tgtcgcggcc gacggtgacc gtcgtgtcta cataggtgtt attcggatcg atgatggaaa
    17881 cgccatcggt catgagttca tccagtttac gctggcgcag gacccgttcg gcttcggcca
    17941 tctgctgacg cgagtttaca ccgagtgttt ccgcatagga cggcgcagca aaggcactga
    18001 cgactttgcc tgccgatacg agcatgccga tgatatcggt cagatagtat tcaccttgcg
    18061 cgttgtcatt ggtgacgttg tgaagcatat cccacaagag agacatatca aagcagtaaa
    18121 tgccggcgtt tacttcgtcg actgccagtt cttccggcgt gccgtctttc tgttcgacga
    18181 ttttgacgac ctgccctttt tcatcgcgga tgacgcggcc gtaaccggtc ggattcggca
    18241 tatgtgccgt caggaccgtc gctgcagcgc cgctctgttc atggcagtcg ataagggctg
    18301 taaacgtttc cttcgtgacc agcggcgtat cgccacaggt tacgaggagg gtgccagctt
    18361 ccccctggag cagcggttct gcctgcagga cagcatggcc tgtgccgagc tgttctttct
    18421 gcatgacagt ttcggcctga tcaccgaggt aatcccggac cgtttcaccg ccaaacccga
    18481 cgacgacgac ctggcgctgc gtcccggctg ctttgacagc ccgcagtacc tgttcgacca
    18541 tcgggacgcc gccgactttg tgcaaaacct tggggaactt cgacttcata cgcgtcccct
    18601 tgccggcagc gaggataagt gcaatagtat ttttcatcca gttatagcct ccctgaattc
    18661 cataactata taatgtccat ttaaggataa cacaaaacgg ggcgaaaaaa aagaggctgt
    18721 cacataaaga cgcctctttt atcaaacaag ctcgtattaa cctaatacat agacaacgac
    18781 atcacgacgg ccgaagtcga gggcttcgct ggtcgaatcc atggccaggt cgatgcggtc
    18841 accgacgata gctccgcccg tatcggcagc tacggcatag ccatagcctt caatgtagag
    18901 ctgcgagccg aggggaatga catcggggtc gacggcaacc atgccgcgct ggagatactg
    18961 tcccgttgcc gtgtagccat caccgccgcc atcttcagcg gtgtaagccg tagcttccat
    19021 gacaagacga cgctgacagc cttccgtcga agacgattct tcagggatcg gacggccact
    19081 ggcttccgac agggcttcca tggtctggga gccgacgacg ccgtctgccg tgaggccgtg
    19141 gtcagcctgg aactgttcga cagcagcttc tgtcgaggga ccaaaatcac catcagcact
    19201 accggacaga tagcccgtat cctgaagcat atattgaacg ctctgtacga cagagccact
    19261 catacccatt tgaacgctag cacaagccag ggatgtaaac gcgaacatgc agattgttgt
    19321 cattaatatc agtatacgat ttctcaaaaa accacctctt tgaacgaaca caatatcaag
    19381 tacgtcctga attataacaa acttcccact ataagtcaaa aaaattaata attataatct
    19441 atttttgatg catctattct ttattctgta caccgtttaa acagcgtatg tccgttaatg
    19501 ctcaacttcg agatgtattt tttagtacct ggtactagtt ttttataatt attatgcatt
    19561 tttacctatt ttagctcttc gtcttctcca caatatttct tatggattca gccccttcta
    19621 tggaatcggt gaggacgtaa atgaagtctc ctgcccgcag acacgtatcg tcatcaggga
    19681 tgatatctcc actgccgcgc ttgatgtcga cgatgaccgt atgggccggc caggcaatgc
    19741 ggccgacata tttcccatcg gcctgactgc cgctggatac ggtcagttcc aggagattac
    19801 gccgttcttc cgacgataag acgggcttat tcttggccag gttccgctgc aataaagctt
    19861 cataaatcgg ttcgccctgg cagagctgag ctacgaccag tgcgaccaat gacgccgtac
    19921 agaggacaag caggtgctca taagagctgg tcatttccat gatgagaatg gttcccgtca
    19981 ccggtgactg aacagaggcc gcaaaaaagg cggccatgga gatgacgacg atgttggaca
    20041 agtaatacga agaaatgagt cccaagcgga caaagacgat gcccgtaatg ccgcctgtca
    20101 gcgcgcccag gaccagcatg ggcaggaaaa agccgccagg gacgccgcag ccatagctga
    20161 ccagggtgaa gaggaacttc ccgatgagaa ggccggcaaa gagtttcagc gataagggca
    20221 gggtatagag gctgttgacc aggtcgttgc cgccgcccag gacttccggg aagacatagc
    20281 ccaagatgcc ggccatgcac aaggcaaagg caatgcgcat ataattattg cgaaagacag
    20341 gtaaatcata gaaataatga atattcagca aacccttgtt gaagacgacg ccggccaagc
    20401 caataatgat tcccagaatg accacgagcc acatgtagcg caggggaaac ggcggcagca
    20461 tgccgaaatg gaagaccggc gcccggccga agacgtagcg gctgaccatg gtcgccagca
    20521 gggccgctgc catggacggc gccaggacga cgccggaaaa attgcggtgc agttcttcca
    20581 gggcgaacat gacgccggcc aagggcgcat taaaggccgc cgccagaccg gcactggcac
    20641 cggccgtgat gaggtagcgt tcttccatgc gcgtccgccc caaggcccgg ctcaggccct
    20701 gagccgtcac ggcgccgatc tggatggacg gcccttcgcg gcccagggac aggcccatgc
    20761 cgatgccgac gatgccaccg atgagcttga cccagagaat gcgcagccag cgcatgcgga
    20821 tggcccccaa gatgacgcct tttacctggg ggatgccgct gccgccggca ttgggttcat
    20881 aaatccccag gtgccataag agcagggctg ccgccaataa gacgccgaac caggacaggt
    20941 tcaggagtac gccgccgctg gccaggacgt tggcatagat ccattggcgc cagaccgtcc
    21001 ccatttccag gccccagcga aaaaagctga taaccgttcc tgataatatc ccgacgacga
    21061 tgccttcaaa aaaaaggcgc aggcggaacc gaggccaatg gtcgagggcc cgcagagccg
    21121 cttctaactg tgtatcgaca tgcatatctt tcacaatcct ttctattcat atgtccaaat
    21181 tatatcatag tcccgtaact ttcgcaggtt cctgatggta tcccctatta tagtattata
    21241 gagaaggcga aatataagtt ttcgtaaatt tttcgcaagc atttcctaaa aatcgttgct
    21301 atttaaagaa tgactcatta tgatagaaac aacacatcga taggagtcct gtccatgaat
    21361 cagccatctt tccatttccc aaaaagcccg cgggccaagg ccatcgcctt tggcctcatc
    21421 tttctcctga cttgtatagc catttatctc tatagccatt ccagtgccgt ctcaaaagta
    21481 aaaggcacgg cccagaacgt atcggtcacg gcgtataccg tgacccgcca ggatatgaag
    21541 cgcaagattt ccctgtccgg ccagaccgtt cccgtcgccc aggtcgacat ttccacgaaa
    21601 tacgccggta aaatcgccga tgtcgccgtc aacctcggcg atgtcgtcgc gccgggccag
    21661 gtcctgctca tccaggatac ggtcgacacg aacctgaccc tcaatcagga taaagcagcc
    21721 ctggaacagg ccgtcgccga ttccaaggct gcggaatcgc aattcggctc agacctgcaa
    21781 aaagcagaag tcgaatacga cacggccaag atgaattaca accgctatgc cgtcctgaaa
    21841 aacgaaggcg ccatttccca gcaggaactg gatacagcct atcaggccct gatcgtcgcc
    21901 aaatcggccc tggacaacct ggagtcgcag aatgtcggcg acgtcccggc cagtgtggcc
    21961 agtaaatatg ccgcccaggc caaagccggc tataccgtcg acagcctgac ccagcagctc
    22021 gacgacatga ccctgcgggc cccgcgggcc ggcgtcgtca cctaccgcaa cgccgaagtc
    22081 ggcgccatgg ccgcggccaa cacgaaagtc atgaccatta ccgatacgag cggcgtatac
    22141 atcgactgtt ccctgtccga agccgacgtc gccgccatcc agcaggggat gcccgtcagc
    22201 gtatccatcg agtccctggc ccaggactac gacggctata ttacctacgt cagcccggcc
    22261 atggacgata cgaccaagac ctacgtcgtc cgcatcaccc tggcccagcc ggacgcgaag
    22321 ctgcgcggcg gcatgttcgc ccagtcgtcc atcgacgtcc tgcagaaacc ggataccctg
    22381 ttcatcccca aagacgcctt gacggaacag gatgggaagg ctcaggtctg cgtcatcagg
    22441 cctgacgata cgattgaact ccgcacggtc acgacgggcc tgcgcaacga cgattatatc
    22501 gaaatcacgg acggcctcga tgacggtgac gtcatcgcca caaccaactt ggcccgcctc
    22561 aaagacggta cgaccgtatc catcgaagaa aggcagtgac cgtcatgaat ttgactaagt
    22621 tttctatcga ccgccctatc ggcatcacta tggtcatcgc cttcttcgtc gtcctcggcc
    22681 tgttcagttt ctaccgcatc ggcgttgaac tgctgccggc cttgaacaca ccgtacgtca
    22741 ccgtgtccat ttcctacccc ggtgccaatg ccgattccgt cgaacagcag gtcgtcaagc
    22801 ctgccgaaga cgctctgtct tctgtgtcta atttaaaaca tatgacgtcg acggcatcgt
    22861 acggccaggc ccggattacg ctggagcttg atttttcggc caatgccgat gcggcagcca
    22921 ttgacgctac gaaaaaaatc aatgccatcc gcggcagcct gcctgacgat atcgacgagc
    22981 cagtcgtcat caagcgcgac gtcaacgcga cgccgatcat gtacgtcgcc gtccaatctt
    23041 cccactccct ggacgatatc tacagcaaga cagaaaatga tttccaagat gtcctccagc
    23101 aggccgatgg cgtctcggaa atcgagctca acggcggccg tgataaggaa atcgccgtcg
    23161 aaatcgacaa ggacaagctc atccactacg atatgaccct ggcagaagtc gtcaaagcct
    23221 tgaaagcgga aaacgtcctc atgccgtcgg ggacgattta ttcgtcaggc acgacgacgg
    23281 acgtccgcgt caaagcccag tacgccgatg aaagcgaact gtcccaaatc caggtcacca
    23341 atgccaaggg tgccaagatt cccattacgg cggtcgcctc catcaagcgc caggaccagc
    23401 gagtcacgcg ttatgcccgt gtcaacggcg acgatgctgt cgtcatggcc atttacaaga
    23461 acagcgacgc caacgtcgtc aacacggtcg acaatgtgac caaaaagctg gacgaactgc
    23521 gccaggacaa tccggactat acctttacca ttaccaatga atcggcttcg tatatccgca
    23581 attccctgca caataccctg cagaccctca tcgaaggcct gtgcacgaca gggctggtcc
    23641 tgttcttctt cctccggggc tggcgatcga cgctggccgt catgattgcc attccgacat
    23701 ccctgatttc tacttttttt gccatgtacc tggccggctt cacctttaac atgatgtccc
    23761 tcatgggcat ggccctgtgc atcggcatcc tcgtcgacga ctcgattgtc gtattggaaa
    23821 acatccaccg ccacctcatg cagggcgaag caccaaaatc agcagccctt cacggccggt
    23881 cggaaattgg catggctgct gtcgccatca ccctctgcga tgtcgtcgtt ttcctgccta
    23941 ttgccttcat gacgggcatg gtcggccagt atttcaaaca gttcggcctg accatcgtct
    24001 ttgccacact cttttccctc ttcgtttcct ttacgctgac gccgatgctg gcatcgcgcc
    24061 tgttccgcct gggcctgaaa atcccggaca aaccgatttg gcataaaatg gacgccatcg
    24121 aagaaagcgc ccgcaaccac tatacgtcta tcctgacctg gagcctggat cacgccaaaa
    24181 agctggtcat ccccgtcctg gtcctgttta tcgccgcagc ggccctgatc ccgacgggcc
    24241 tggtcggttc ggaattcatg ccccagactg acgaaagcag tttccgcatc accgtcgaac
    24301 tgccgaccaa tgccaatctg acgcggacgg ataaagtagc gcgccagctc gaagattact
    24361 tgaagacggt cccggaagtc acgtactata cgtcgatgat cggcggcaag agcctgaacc
    24421 aggccaatat ccaggtaacg ctgaaagacc ggcgcgaccg gtcgcggacg atatggcaag
    24481 tcaccaacga cgtgcgccgc tttgctgcgg accacatcta tgacggcgac gtccgggtca
    24541 aagaagccca ggcttccgta tccgggacga caggcggctt cggcggcggt tcaggcaacc
    24601 ttcgcctgga actgcgcggc aaggacagcg aggccctgct ggcccagtca cgggctatcg
    24661 aacagatgat gaagaccgac atcgccggcc tgcgcgacat cagcagctcc taccaggacg
    24721 gcatgcccga ataccagctc gtcgtcgacc gtaataagct gaaacaatac ggcacgtccc
    24781 tgagcgatct gaacgacacc ttttccaatg ccatcagcgg ccagaaagcc ggcgtcctgg
    24841 ccaatgacgc caaaaacgac aacaacgaca cggatatcta cgtccgcctc aaaggggccg
    24901 acgccttccg cctgtccgac ttggaaacga ttcccctcaa cgccaacggc cacatcattt
    24961 acatcgccga cgtagccaag gtccaggccg ggacaggacc ggtgacgatc cgccggaccg
    25021 acaaggaacg gagcatcacc ctcggcggct cgcctcaggg acggccgctg aacgaggtca
    25081 tccaggacgt ggcgaatcac ctgaacgcca tggacctgca cggcgtgact ttccgcttca
    25141 ccggccaggc cgaccagatg acgacgacct tcacggactt gggcgaagcc ctgctcctgt
    25201 cgctcatcct gatattcatg atcctggcca tcctctatga atcggtcaag acgccattca
    25261 tccgcatgtt ctcgctgccc ctgggcctca tcggctccct gttcctgctc ttcttgacca
    25321 ataatacgct gaacctctat tcccttatcg gcatcctggt catggatggc ctggtcgcca
    25381 agaatgggac gctcctcctg gactatacgc tgaccctcat gcacgaacag ggcctggacc
    25441 cgcgcagcgc cgtcatccag gccggccgcg tccggctgcg tcctatcttc atgaccaccc
    25501 tgaccatggt cgtcggcatg ctgcctacgg ccctgtccat gacggccggg gcggaaacgc
    25561 gggccagtat ggccgtcgtc gtcatcggcg gcctgctgac atcgacagtc ttcacactgg
    25621 tcattatccc gattcttttc gtcttcatgg aaaagcacag cctgttccga cgaaagagtg
    25681 cctaaaaaaa gtcctgccct ggtggcagga ttttttgttt tattatagaa taatatatac
    25741 atatatagaa aatgcatcgg gctcaatgaa ggaggactga cctatgaacg atagatacac
    25801 accgacattt aatattcacg aagccgtgcg caagacttta ccttatatga tggaaatgcg
    25861 cgaatatttc catgcccatc cggaattgag tgaaaaagaa ttcgatacgt gcaagacgat
    25921 attccaggaa ctgtccctca tgggcatcga agacgttaaa atcatcgccg gcacaggcgt
    25981 caccggtctg atccacggcg ccctgcccgg tccagtcatc ctcttgcggg ccgatatcga
    26041 cgccctgccg ctgacggaaa aattcgactg cccttattcg tccctcgtcg acggcgtcat
    26101 gcacgcctgc ggccatgacg gccatgcagc caatctcctg ggcgtcgcca aaatcctcaa
    26161 caagcacaaa gactgcctgc gcggcacgat ccgcctggcc ttccagccgg cagaagaagg
    26221 caacggcggc gccgcccgca tgatcgacgc cggcatcctc aacaaccccc gcgtcgacta
    26281 tgccctgggc ctgcacgtcg ccggcagcct gcccaagggg tacgtcggca tcggccgcgg
    26341 tgagatttcg gcgtcctgtg acgacttcac catcaccctc cacggccgcg gcggccacgg
    26401 ctcccagccg tccctgtgca tcagccccat ccagatgggc atcgacatca tccagtccat
    26461 ccagaacttc gtctacaact gccatgccga cggcgacggc gtcgtcctca ccttcggcca
    26521 gttccaggcc ggccacaacc cgaacgtcat cccggaaacg gccgaactga aagggaccct
    26581 gcggacatat aacgaagaaa agcggaaacg catcctcgac gccattcagg ccatcctgaa
    26641 aaatgtcact accacttacg gcggcacctt cgacctggac gtcgtcccct tctcgccagt
    26701 ctgcgtcaat gacgacggcg tcaccgaccg ggtcgtccac atcctccagg accactgttc
    26761 ccaccgcgtc gacctggtcc ccttgaagcg cggctccggc tcggaagact tcgcttattt
    26821 cagcgccgcc gtgcccagct cgttctacta cgtcggcgtc tacagcgacg caccggtcat
    26881 cggccattca ccggaattcc attgggacag caatgccttg aagtacatgt gcgaaacgat
    26941 gctcaccatc gtctattact ttgaaagtta tgcctgagct tcccagttgt ctaattccat
    27001 attgagcgta taatgagggg tatccatctt gaaaaaggag tgatacccct catggtattc
    27061 tggttcatca tcgaacacac gatgcttccc attttctttc tcgtcgtcct cggttatttc
    27121 ctcgataaga aattccacct cgacgtaaag accttatcga agctcatgtt tttcctggtc
    27181 atccccagtt tcatctttac caatatctat acgacgcaat tcccggcgac gagcatcaac
    27241 atcatcgcgg ccatcgccat cttcatggtc atctgcttcg tcatcgccaa catcgtcggc
    27301 cgtatccgcc actacgatgc agccatgctg gaatcgtgcc gcaatgcctt catgttcaac
    27361 aacacgggaa atctcggcgt cgccctgatc atcctcgtct tttcccacga ccccttcgtc
    27421 gtcaacggcc agacgccgta tctggccgaa gctatggttg tacagatcat catcttcatg
    27481 atccagagca tctccctcaa cacgctcggc ctctatcagg ccggccgggg ccgcctgacg
    27541 gccagggata cgctatctgt tatgttccac atgcccctgc tctatgtcct ggctgcggcc
    27601 ctcatcgccc gctacgtcgg ctgggacgcc aaggacttct tcctgtggcc tatcatggat
    27661 atggccagca aggccctcct gcccctggcc atggtcagca tcggtgccca gttgtcgcag
    27721 acgaagatcc aatggctcga caaggacgtc tggattgtca gcatcctcaa gatgacggtc
    27781 ctgcccctca tcggcctggc catgatctac ctggccaatg ccttccggcc cggcacgttc
    27841 acagccgtca gtgccacggt cttcctcatc tactgtgcca tcccgacggc cgtcaatacg
    27901 gccctgtttg ccatcgagtt cgacaacaac cccgattaca ccacgcaggt cgtcatgaac
    27961 acgacggccc tgagcgccgt atccctgact ttctatattt tcctggggca cattcttttc
    28021 gtctgaatga tatacttttt atctgttcgt tactaaaatc aatatatccc aacttttatt
    28081 aaaagaattt caaaaaaagt tattgacatg acgcgttttt acttatataa taatttcatc
    28141 aacagaatag cagcggcaaa ggagcctgga aagagagagc ataaactatc tttccaggct
    28201 tttcgtatat tctggcgata ataaaatatg acagtagaca atgatgtctt ttccccgatg
    28261 gttccatcag gaaaggcatc ttttttattt gtcaggaggt tgggaattat gaaaaaatta
    28321 tggtcacgtt taggagtgct gctcccgtgt ctgctcatgg cagcgccggc agccttcgct
    28381 tccgatccgg ggacgatcga tacaggcgat acggcctggg tcctcatcag cgcagccctg
    28441 gtattcatca tgacaccggg ccttggcttc ttctacggcg gcatggtccg caacaagaac
    28501 gtcttgacga cgattatgca gagcttcttc atcgtcgcca tgatttccgt agaatggatc
    28561 ctcatcggct atgccatgac tttcggtacg gatattaatg gtttcatcgg ttctcttgat
    28621 aaagtcggtc ttaccggcgt cggcatggct gtcctggaaa acgggacgat tccggaaatg
    28681 gctttcgtcg ccttccagtg catgttcgcc gtcatcacgc cggccctcat taccggcgct
    28741 ttcgccgaac gcatgaagtt ccacgctttc gtcgtcttca tcctgctctg ggctatcttc
    28801 atctacaacc ccatggcaca ctgggtctgg ggcggcggct tcctggctaa actcggtgcc
    28861 ctcgacttcg ccggcggcct ggtcatccac atcctctccg gtgtttcggg cctgaccctc
    28921 tgcgtcctcc tgggcaaacg ctatggctat gggaaaatcc ccatggttcc gcataacctg
    28981 cctatgaccg tcctcggcgc agccctcctc tggttcggct ggttcggttt caacgccggc
    29041 agtgctctcg gcgccaacgc actggctgcc agcgcgttca tcgcttctca ggccgctgcc
    29101 gctgccgcta cggtcacctg ggtcgctgta gaatggatag tcaagggtaa accgacaatc
    29161 ctcggtgccg ctacgggctg catcgccgcc gggat
//
LOCUS       DAWCYS010000004        28061 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_98_length_28061_cov_12.128472, whole genome shotgun sequence.
ACCESSION   DAWCYS010000004 DAWCYS010000000
VERSION     DAWCYS010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 28061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28061
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_28061_cov_12.128472"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6526 a         7210 c         7442 g         6883 t
ORIGIN      
        1 cgttcaaagc gtcagtacca gcgttcggtt cggtgaggcc gaaagcgccg agtttcgtac
       61 cttctgccaa gggtttcaag tatttggctt tctgttccgg cgtaccgaat ttccagattg
      121 gccaggagca gagcatggtg tgtacttcga agctgagtgc gatggacggg tcaactttag
      181 cgacttcttc gcaagcgaca gccaggctga ggaagtcagc gcctacgccg ccgttttctt
      241 cttcccaggg gatgccgaga aggccgaggg tacccatttt gtcgaggata gcgcggtcaa
      301 agacttcctt ttcatcgcgt tctttgacag tcggtgccaa ttctttttct gcaaaatctt
      361 tagccagttt agcaatatcc tgctgcattt cgctgagttt gaaatccata gtaaatattc
      421 ctcctaaaaa taattcttat tacatactaa aggtttatag gaaagggact gtcgcacgaa
      481 gctttcagct gccatgcgaa gcccctcctt ctaacaatga agcgtgctct attatttgcc
      541 ctggaagtgt ttagcgcgtt tgttgacgaa tgcgtccatg ccttccttct ggtctgccgt
      601 ggagaagcag agggcgaata cttcgtcttc gtaagcgata ccggtgacga cgtcgcagtt
      661 gatgccgcgg ttgatagccg tcttagcgag ctgtacagcg ataggagcgt tgcccatgat
      721 ttctttcgct acctttttgc aggtatccat caattcttcc tgaggaacga ctttgttgac
      781 caagccgatg cgatgagctt cctgagcgtc gatcatgtta gcgcggaaaa tcatttcttt
      841 gccatagcca cggcctacga cacgggggag acgctgagtg ccgccgaagc ccggtgtgat
      901 gccgaggcca acttcaggct gaccgaattt agcgttttca gcagcataac ggaagtcgca
      961 agcgcaggcg agttcgcagc cgccgccgag agcgtagccg ttaacagctg caatgaccgg
     1021 ctggggcagg ttttcgatgc gtgtgaaagt atcctgggaa atcttaccga atttacggcc
     1081 ttcgacaggg ttcttagtgg acatttcaac gatgtcggca ccggcgacga aggatttagc
     1141 accttcacca gtgatgatga caacttttac cgattcgtca gcttcgattt tgccaacgca
     1201 gtcattcagt tccgtaactg tttccgtgtt caatgcgttg agagccttcg ggcggctgat
     1261 cgttaaaata ccgataccat cttcgttgac gaatttaata ttgttgtaag ccataatgaa
     1321 aaacctccta atatagttat aaaaattatc aactatgtct tattgattgg ccttgccttg
     1381 aaccaacctt gtgactacag tatacaaatg accaagttgg aacaaaacag gaaaggcttg
     1441 accttttttg catagaatta agtccatgtg aatttttttg tcaaaatcat tgtgattcac
     1501 taaggtatgt gataaaatgt acatgctcga ttgaattaaa tttatacaga gaatgaatgt
     1561 atttcatata tattaaaagc catatatgtt ctatataaaa aatatgacaa tttcgtattg
     1621 aaggaggtta caacgtgatt tcagatggat atgctttaac gttaaaacgg ctcattacct
     1681 tcacacaggc caagttcatt tccctggccg atgtcgtcgg ttacgatgtg tcctacgtca
     1741 ataaatggag caacggtacg aaattaccgt cttcccgtta tgtcgaacgg atcaacgaag
     1801 aaatgggcca atactttgcc gaactgatca ctaagcagaa gaaagaggcc aagttcttta
     1861 aaaccttccc catttccgaa aatacggatg acctgggctt tgaaatcggc cagtacctct
     1921 gcgccgccta ccggacgacg ctgaaccaga accgggttcc caagggcaaa gaaaaccgcc
     1981 cgtccatcca ggtcgtcacc ggtcatcacg atacgtcggc tttcttgtcg gacttgctgc
     2041 aaaagggcat tcaaagcctc gaaagtgatg gtgagctcct ggtcctcggt gaattttgta
     2101 ctttatacaa aacagggttc tggaaatatt tcgaaggcct cgaattacag cacagactga
     2161 ccatccgtgt cggcctggac ttagataaaa ttgaaagcaa cttcgatgat ttaatcacgg
     2221 tctatcgtac gctagataac tttttggata tcgatttcgt cttttatgac atccaggata
     2281 cgtccaatgc caacctcatc atcctcaagg gcgcgttcgt cgttcaatat gccctggaca
     2341 cacagccccg cttcaagatg tgtacctata ttttcgacga gtccttagtc ggtgatattt
     2401 atgaaaaatt cagtttcaac ggagctgaaa agaaaccgct catgtcgacg gtcagctctc
     2461 tgggactgga agaactcggc tatcgcacgg ccttctatgc gacaacgaaa ttcttcttct
     2521 acttgaccat cggctttgaa ttcctccttc ctcacgaagt cttcgaccac atcagcgaaa
     2581 ccgtatcgcc ggaacaggcc tttgccatcc agcgcctctg cgtcacgtgg gaagaaatcc
     2641 tcaatgcttc ggaaatcacc ttcatcatgc cgacgacatc gctcctgcgc tacgtcgaat
     2701 cgggctacat ctacctgacc gacatcgaat accgcttgac tgcggaagaa cggaaagcgc
     2761 atatcaagag tatctttgaa ggcctggaaa agaatcccaa cttaacgact ggcgtcctct
     2821 tgccgaccgt cagcgacggc atgtacacgg gcaaggactt gtccttctat tccaactaca
     2881 agacaggctt cctgaaaaag aacaagcgga atatccacaa cgacgccgat tcgttctacg
     2941 tcatcagcag caaccgtctc cactccatcc tgctcaatgc cttccagaac atgaagaatc
     3001 agccgtcata ccgccagtat gatttagaag ccttaaagaa aaaatacgaa acgtataaac
     3061 cgctcatcga aaggaccttg tccttgtcga agtaaaaaaa gaggctgtcg cattaagaca
     3121 gcctcttttt ctgtaggggc gcccacgtgg ggcgcccgcc catgcaaatc ggctacaggc
     3181 atcataacat atacctgacg ggctcgccac gtgcgagccc ctacaatcaa aaaaagaagc
     3241 ttgtcgcaaa tgcgacaagc ccttttttta cagtccgcaa ctcacagcgt agtccgcggc
     3301 tgtgtcaaaa cctctcaggc cgccttcggc ggcctgctcc cctatcaggg gagcttttct
     3361 gcaaactaca aactctgtac tacaaactat tatttgttca tttcttcttt catctttttc
     3421 aaacccatgg gtttatcttc aaagaggaag gccggcatga ctttgacttc gtcgatgatc
     3481 ggcgtgaagc ccatctggtc gaggacgtct ttctggaggt cgatgcccgg ggcgatttcg
     3541 gtcaggtgga caccgtcttc tttcatttcg aatacggcac gttctgtgat gtagaggaca
     3601 tgctgtttat gtttacgggc atagttgccg ctgaaggtga tgtgaccgac ttcgggaacg
     3661 aatttcttga ctttcccttc ctgcaggatg tgcagttcac cgtctttgat ttcttcgcgg
     3721 agtttccctg tcgtaaaggt cccgcagaag acgacgttct tcgtattctg ggtgatgttg
     3781 acgaagccgc cgcagccggc aatgcgcgta ccgaatttgg atacgttgat attgccttct
     3841 ttatcgcatt cggccaggcc caggcaggtc aggtccaggc cgccgccatc gtagaagtcg
     3901 aactgtgtag cctggtccat gtaggcttcg gcattgacag aagccccgaa gcggacgccg
     3961 ccgagaggta cgccgccgat ggccccggct tcgacagtca tggtcaagct gtcgccgatg
     4021 ccttcttcgt tggctacgga cgagacgagt tccggaatac cgatgcccag gttgatgacg
     4081 gcttcactgc tcatattgag aagcaacagg gctgcccggc gggcaatgac tttcttggca
     4141 ttgagcggag ccggatccag ggctttgacg ggaatcgtcg tttcgcccgt cagggacggg
     4201 tcatattcgc agtcgaagga ctgctgccgt ttcgtttcgt catcgacctg aacgacgtag
     4261 tcgacgtaga tgcccggtac tttgaccagt ttcgggtcga gcgtgccgcc tttgacgatt
     4321 ttttcgacct ggacgacgac gataccgccg ctgttatgga cggcctgtgc cagcggcgtg
     4381 gcatcgaaag gcgatacttc cttggtcagg acgacgttgc cccattcatc ggcataagtc
     4441 ccgcggatga agccgatatt catatccatg cgcggataga agagctggtc atggccttcg
     4501 atgttgatga ctttgacgaa gtcttctttg gtgacgtcat tgagacggcc gccgctgatg
     4561 cgggggtccg caaaggtatc gaggccgacg tgggtaatcg tgcctaagcg atgagctgcg
     4621 acgtcacgga aatactggga cagcgtgccc tggggcaggt tgtaggcctg gatcttattt
     4681 tcattgacta atttctgcag ggccgtaacg gcgttccagt ggccgaggat ggcttttttc
     4741 aacatccctt cgtgggcgaa gtggtcgccg ccgcggccat ccgtattccc ctgggaagcg
     4801 gcatagatga gcgtcaaatc tctcggcgaa cccgtattga ggaagcgttc ttccagggcg
     4861 ctggtcagcg cttccggctg tccggaaccg acgaaaccat tggtcgtgac gacgtcaccg
     4921 tctttgacga gctgagctgc ttcttgagct gtaattattt tcacttgttt catgtcttcc
     4981 atctcctatt tcttcacgtt tgttatatgc atttcgtgaa ttcattatac aaaggaagca
     5041 tatggaatga tagacgaaaa ggatggacta aattggtagg aaaaaattcc atgaagtcaa
     5101 attactgggc ttccagggca ttccaggctt catcagctcg ttcgacgaac atctggcgga
     5161 ccgcttcatt ttctcccagg ccatggatat aggtcgtaac cgtgaagcct tctttttcca
     5221 ggatactctt atgggaatcg gcatcggcac cggccatgtc gttattggca tggtcgccgg
     5281 cgaccatcat catgggcatg aggatgacgt gcttgatgcc cttggctttc agctggggaa
     5341 tgactgtatc cagatggggc cagccttcga cagagtagat gtagacattg cccagatcag
     5401 ctgcatcgag gcggttctgg acgacggagt aataagcatt ggccggatgc ggcgtgccat
     5461 gggccatgac caggatggcg tctttcttgc caattttcgg gaactgtgtc gagaaggctt
     5521 tgacgaattc agcgatgtcg tcgcgctggt cttcctgtcc catccaatac atgagcggcg
     5581 tgccgagggt caatttcttg aaatcatgtt tgtagaggtc gaaaatagcc gtatcataag
     5641 catattccat acccgggatg atgtccaggg aggtaatggc cacgcgcgta tagccttctt
     5701 ttttcagctg ggccatggcc tgttccggcg tcggtacggt cacgccttct ttagccttga
     5761 tgcggtcgac gatgatatgc gacgtgaagg cgacgacgac tttctgatcc ggatgggccg
     5821 ccttgatagc cgatacagtc tggtcgatgg tcttctggcg gctgtcctta taggtcgtgc
     5881 cgaagctcaa taccaggatg gcgtctttat tggcttcgtt ctgcatgtcc gggttgttgt
     5941 acgtgcggac cccgatttcc gcggcctttt tcaaagccgg cgtagcgtcc ttgacttccg
     6001 ggttcagcgt atagccggca gcaaaagagg gcgccgaaaa agcaccaaac gacgtcaggg
     6061 ccaaagataa aatcagcgcg gtctttaatt ttttgtggaa cataatcatc tctcctttga
     6121 taaaaaatat aaaaaaagag gctgtcacat gatggcataa gccttcatgc gacagtccct
     6181 tacatacgag tccttcacgg ccagcagctc ataaaaagcg gctcgccgta aaaatcgcca
     6241 tccccatcgc tcgcaggtca accggtgatc ctaatcaggc agttcttctg gctcggattc
     6301 atggccatgg ctgcgccttc ccatacgcgg acgtacagtg acatcattgc agcctggctc
     6361 ctccttacag cggcgggacc gcgccggttt tgcaccggac ttctctattg agcttacgca
     6421 cctgattgca ctatggaatt gtatgaatcc ttacaggact aacgtcgggg cgacatacgt
     6481 acgggccagt aattcctgat tcagggcata gaggcctttg gcatcgctgc cgaacatctt
     6541 gtatttcttg atcaaatcgt cggcattctt ttcttcttcg ccctgttcct tgacgaacca
     6601 gtcgaagcac tgcatggtcc ggtaatcgtg gactttatcg gcagcctgat aaatcttgtt
     6661 gatcagcgac gtgacatact gttcatgagc caagccagct ttgaggaccg ctttgatgct
     6721 gtcaaagtcg ttctgcggtt cggcgatggc cgagaaggta acgcgttcgc cgttgttatg
     6781 cagatatgtc cgcatgagca tcgcgtgatc ccgttcttcc tgggcctgga catcatacca
     6841 gttggcaaag ccgtcgaggc cattgtcata gaaatagttg gcaaaagcta aatacaaata
     6901 ttccgaatag aattcctgat tgatctgggt attgattaat tcttttactt ttgcatcgag
     6961 cataacggta cctccttgag atatgtctca aaacgtgtac agaacttcat cggttttctt
     7021 gaaccgtcct tattatatca catttgaccc tatcaggaat acttcttatt tgactttttg
     7081 ctcatcaatg gcattcttga tgctcttcaa gtccttgata tcgacttctt tcaggagctg
     7141 gcggaaccgg gccagctgtt tttcgacttc cggtgacaat ttcgggaatt tcgggttaat
     7201 ggccttgagt tccttgagga cgatctgggc gacgacatag cgcgtatacc acttgtcatc
     7261 agccgggacg atgtaccagg gagcgtactt cgtcgacgtg gccgataaga cttcgccgta
     7321 gactttctga tagcggttcc aatattgccg ttcctcgatg tcagacatgt cgaacttcca
     7381 attcttctgc tgagtcagga tgcggtcgat gaggcgtttc ttctgttcat ctttcgataa
     7441 atggaggaag atcttgacca tagggaagcc attttccgac aagtaccgtt cccagtcgca
     7501 tatctggcgg tagcgcgttt cccagatatc cttgcgtacc agtttttccg gcataccgtc
     7561 gcttttgatg aggttgtgga tgcgggcgac gacgacttct tcatagtgcg agcggttgaa
     7621 gatgccgatt tcaccccggc cgggcagggc tttattgatg cgccacatat agtcgtgatc
     7681 cagttcttcc gagctcggtt tcttgaagct gtgcactttg acgccagccg gattgaggtg
     7741 ggcaaagacg tgtttgaccg taccgtcttt cccggccgca tccatggcct gcaggacgat
     7801 gatcaggccg tagtgattgt cggcacagag cttttcctgt aaatcctgca tttcggaaat
     7861 cgtctggtcc agcagttcgc ctttgacctt cttcttgtcg atgtccacat cgcaggtcgt
     7921 cgaccactgg gtcaaatcga tttttttgcc ttcttccgct aaatatcggg aaatatccat
     7981 agtaaaaact cctttctcta tctatagtgc ttctcacaag actggctgtg ggccgccttt
     8041 tccccagcgg ctccgcttcc cgtttttata gttgatgttg atggacccta tcttgacttt
     8101 gccattgaca atggaaaaag cgtccgtcga tacgtcacca aagaacatgg aaatgacgac
     8161 gtcccggaag ttcaagatct tgccatcctg agcgaaagag ccggaaatgc cccggaaggg
     8221 cagctggtga tagcgtcccg ggccggactg gaaatcaccg taaatcgcct tggtgccggt
     8281 cgttttattt tcttccatgt gcagattcaa gtcgacgttg cagcgcagga agaggtcatg
     8341 ggccgcaatg ccgcctttga gctgggagcc ggtcatgtcc gccgtatacg atttatcgtc
     8401 caggtttacc tgtccatgac ctttcatgga gccgccgaag acgccggccg tgacttcgtt
     8461 tatatcgagc aggccctgtt cataatggac ctgtcctacg acatccgtaa agaccagggc
     8521 cgtaatatcc agacggtcca tcttcaaagt gccgtcgatg accggggcat ataaagggcc
     8581 gcggatgtcg gccgaaactg tcgccgggtc gtcgatatcc aggccggccc cgagagaacg
     8641 gggattgcag tcgctcagca agagcgacag gttatactgc ggtgtttctt ctttgaaatc
     8701 caggctgccg cggatgcgca gggacttggc atcgatcatg ccggaaaagt gaccggcccg
     8761 caagtcgatt ttcgtcttgc ctttagtatg gatggccgaa gagaactcga catcgccgat
     8821 cttgaaataa cggcctggcg cttcagcttc gatgacgctc tgatgcaggg cgacgtggca
     8881 gttaaagggg cggttgctct taggccctgt gaccttgacc aaatccttcg ggccgctgct
     8941 gtgatggtca ctggccttct tgatatgctg taactccatt ttatcattaa agatcaaatg
     9001 aatataggca ttattcaaag tcacgtcttc gacagtctgc gtaccgatat gaccggtcag
     9061 gacatcgcta agtttgaccg taaactggcc ttccggtatc tggaccagcg tatccccatc
     9121 ttcatcgtgc cataccaggc cctcaaagga cacggttccg cctggcgtcg ccgtcaggcg
     9181 ttccaccgtg accgtaccgg gaaagagctg gcgttctgcg acgacttggt tgaagatatc
     9241 cgccgctttg tagctcatgt agaaacagag tccgtaaaga acggcggcgg cgatcaaggt
     9301 aatcgccccc atgcgtttca gttttcgttt atgtcggaca taccatttat tccacatgtt
     9361 ctcccgtctt ctttgcactc atatcactga ttttccaggt gttcccgtct tttaccatcg
     9421 tcacctgacc gttgaaatac tggactttga ccttggcact gcctgggata cggtcccgcg
     9481 ctttcaaggt gaaggaaaag gtcgtcttat cgcttccgga agcgattttg cggagattgg
     9541 aaacgtcgct ggacaaggtg ttggcgtagc ccggcgcgta accatcatag ccgccgacag
     9601 aattctggaa ttccccagtc agcatctgga aggcatcttg caggtcgtga ttggaaatgg
     9661 ccttatggta agccaggaaa gcctgcttcg cgccatctgt cttttcatcg gccaggcgga
     9721 cgctgatata ttcgacggtc tggctgttct tgctgacggc aaagcgcagg attccctgtt
     9781 tcccgtcatc ggtcttaaag gtatattcat aatgaatctg ggcaccgtat tccgaggcct
     9841 ggtaatcgtc accataggca ttgacgacgt catccaaaga gctgccctga tggatgcccc
     9901 gcttgctctg agctgccggc gaggaggatg tcagcatgat gactttatcg tctttgacga
     9961 cgacatccat atcggtaaac ttatgatgaa cggcaccgtc tttactatcc gacgtgctga
    10021 gttcccggcc caggccgtct ttagcttcat cccagctttg gtccaggtaa acgccgttta
    10081 aagagagttc cgaatcagcc gtcttgacaa gctgcgacga tacggtactc ccctgggccg
    10141 gtagggcttt ctgttcatgg gcgtaccaga tgccgccggc aatggccacg acgaggacgc
    10201 aggccagggc gatgaagaga ttgcgatgct cctggagctg cgcttttaaa ggaacttctt
    10261 ttttctcccg ctgcggcata actacaggat gtactgccgg ccggctttca ggctgcacta
    10321 ccttttcctc ctgctgtacg gctgtccgat tggtcagatg accgtattgg gcttgcagaa
    10381 cctgcagcgg agccccgcag gacgggcaga attttgccga cgggtcgacg ggctggtgac
    10441 attgcgggca cgtatggacg ggtttgccgc aatgactgca aaaggccgca tcagccgcta
    10501 cgggacggcc gcaatgaggg cagtattttt catttactcc gtttacattt tgcgttgaca
    10561 ttgcattttt atcgttttga tgattggctt ggttatcgcc tgtatcgtca aagttaaatg
    10621 atgtaaacat gttttcaccg ctcaatgtgc caatcgacgc cagggaagaa atgccgaaga
    10681 tgaagaacag ggctgtcatg ggaacggtac acatgaggcg aaaggcctga tagaaggaag
    10741 tccccagccg ttcttcccgg cgatagatgt aatgctggca gaaaacagga atcatccaga
    10801 ccaggaccag gaggacggca tagccgataa aggtcccata gccgccgcgg gtcatgccga
    10861 acatcagctg ggtcatacta tagacgatat ggccgacgac ggcagccgta caaagggcgc
    10921 tgaccagcca gctgaggatg acgtagtaaa tataacggcg gtattgactg atatagtgcc
    10981 agcgcttgcg attcacataa atagtagcga tgatacctaa gatacagaga atcgcagcca
    11041 cagtcccatt gaaaatttcc gcaatcaaga tcaacaatag gtctacgaca aaatagccca
    11101 ggaacatttt gagcagataa gccagagccc gcgtcttata gagcaggtaa atcatgatga
    11161 ccagaagagc cagcgtgacc agcgtattga ggccgctgaa aaaggtcagg acatccatgg
    11221 tccgccgtac cccagcagga ccaccgaaca agccgatcat agccagtccc cgcagggcaa
    11281 tttcaaataa aacgcccagg accgtcgtca cgacgccaac atacatcatg gcgataatag
    11341 ccgtgtactt cttatgctcc gtcgggaacc acttgccaaa atagcctaaa gaatccatag
    11401 aaacacctcc ataaagataa ggtaattata attttatgtt acacctattt ggcaaaaagg
    11461 aaaaggactt gtcgcatgtg cgacaagccc cttttcgagt attaagtttt cagtttgtag
    11521 tttttagtta aggcttttaa atttaaaacc atctgctaac ggctaacagc tagccgctga
    11581 ctgctaaaaa aggcgctgtc ttcatgcgac agtacctttt agtgattaat ggctattata
    11641 ttttgtcatg tccagtgtcc ccagctgttt atcgatcatg atggagttgg tgacggaacc
    11701 agaaacgttg accatggtcc gcatcatgtc gatgagggga tcgatggcca gtatcgggcc
    11761 gatgaggctg aacgacgagg ccatgccgac gccggacagg ctgactgaag cggccatggt
    11821 cgccgtaccg ggaatcccgg caatgcccag tgagccgatg gtgacgacga tgacggtcat
    11881 gaccagcatg gtaatatcca gcggcgtccc ggtcagatgg cagacgtaga cgatgagcat
    11941 agccgggaag acgccggcac agccctgcat gccggctgtc gtgccaaagc cggcaacaaa
    12001 gctcgccgtt ccctgattga cgccgaggcg ggtaaccagg gtttcaatgg tgaccggcag
    12061 gcagccggcg ctggaccggg acgtaaaggc caggatgagc agggccacgc ttttcttcaa
    12121 gtagtgaatg ggattgacac catgcagcat gacggccagg atctgaatga tgaactggac
    12181 gataagggcg acatacaggg cgatgatgaa gatgcccacg tcgaggatgc ttgccaggcc
    12241 tttctgggca atagtgttag ccagtaaggc gatgaccgcc cagggcatgt agtccaagat
    12301 gagcagggcc atgttcatca tggtcttgtg caggccatcg atgagtttat gcaaaaaggc
    12361 ttccatatcg ggcttatctg ctttgatcca ccagatggcc cggccaaaga cggcaccgaa
    12421 gatgaccatg ccgatgacgt tgttcttgac catggaatcg acgaggtttc ccggaatgag
    12481 agcccgcagg gtcgaagcca cgctgacgac atccttcggt ttggccctgg cagcttcact
    12541 gctgatggca ctgcccgaac cgacatcgaa aaggataccg aagacgatgc cgatgacggc
    12601 ggcaatagcc gtcatgacca aggtcaccag caatgtccgc ttgaccaggc ggctcatggt
    12661 cttgccagaa tccatattga tgacgacgtg ggcaatagaa atgatgacca gcggaatgac
    12721 gatcatcttg atgaggtcga tatagccatt gccaaagagc tggaaccagt tcgtcgtttc
    12781 cttgatgaag acgactttcg tcggatcatc agggaagcca gccatcagct gcatgaccag
    12841 gcccaataaa gcgccggcca ccatgccgac gcagacgacg agcgagaagc ccatcttgca
    12901 cttatgatag agataatgaa taagtccgaa gagaacagcc aggacagcta agaataagac
    12961 tgtccgccaa tcgctgatca tcaggaagga cgtaaaaaat tgactttcca tactgaattc
    13021 cccctagtaa tcaataacca atatacattg attttcttat tatactaaat tggtagtctt
    13081 ttgcaaagga cttttctgct gttttattga tgaaaaaata ggctgtcgta ttaagacagc
    13141 ctattttttg agtgttaagt ttgtagtttg cagtgaatgg ttttaaattt aaagccatct
    13201 tctacaaact acaaactccg tactacaaac gataaagaga aagggaccat gcatgatggc
    13261 agaaacattc gtgcaacagc tcctacgagc cacgagccac taaccctaat accattcgtt
    13321 aatccagttc ttttcggtcg ggaagagatc ctgcagggtt tgcagagcct tttcgtttcc
    13381 ctggagtttt tcgttgaagg ccaggtcgtt tacatccatg actccgaaag ccagcagggc
    13441 cagggtgccg atgggcatgg tgacgtccgg ttgtttattt tcttcttggg ttactttggg
    13501 tttaccattg tttacagtca gggtaaaggt cccgcagttc caatctgcca ggggatcttc
    13561 tgtatggaag gtgacggtcc cgtcagctcg ataaggcagc tgttccagcg cccctttgac
    13621 gtcgacgata cggcctgtca tgaagggcat cgtttcatgg ccctgtttcc cgtcgggata
    13681 gaagcggtag gactggtcat ggatgccttc attccactga aaggcgtcgc cctgggaacg
    13741 gtggttgtac atgtagccta agaggccgcg cttgcctttg cggcaggtgt aaacgaattc
    13801 accgctgatg atggtcggtt ctcccagctg atagaaggca tagccaatgg gttcatcgcc
    13861 atcgaagcag acggcgatat tgccttcggc caactgggct tcgatatggc tccgccagct
    13921 cgcttcatcg cgcagggcat agcctgacag gcctttcgta taaccttcat aaatggcagc
    13981 caggtaaggc cactggtccg ggctgttgac aaaggcatag cgcacggtcc ggtccgtcag
    14041 cggccgcagg gcttccagat tcatcgtctc tttccactga tggcagtaga gttcataacc
    14101 atagggctgg taaaaaccgg ccttgctggg catgaggatg tggatataat gaccgcggcg
    14161 cttcatttct tttagggctg ccgtcagcag tttcccaccg acgccgctgc gccgagctgc
    14221 cgggtgcgtc gccaaaccga cgatatacga cgtgtctacg gctttgccgc gcagacagag
    14281 ggtgtacggg ttgagatggg tcaggcaggc catatcgtcg ccctcaaagc ccatgaggac
    14341 gttttcaggc tgatagaaat ggttgaagta ccactggaaa aaaggatctt cccgatgttc
    14401 aaaacagtag tcccaaaggc tttcgacgag gccccggtcc gattcagccg ctaagcggaa
    14461 gtgcatagaa aagccccctt atttctgacg cagtgtgacg tcgtattttt cgacgaagtg
    14521 atccggattg tacgattcct tggaatggcg caggccggga atccccatat cttcttcacg
    14581 gttgatatac gtcgtatgcg gccaggcatg gacgacgaat tcatgattga tgacctgata
    14641 gaggccgcgg atcgtcggat cgctcttttc aaagtggatg atggccgtat catcgttgag
    14701 catctcaccg atagagaaag cgacgatctt attgtacatg cggatggcac cgcccgtcgg
    14761 ctggaggact tcccagttat tgaagaccgt ttcgatggca tggcgttcgc ccaagagttc
    14821 atcatcttcg tcaccggctt cgtgctggtc gtaccaggat ttttccgcag ccagacattc
    14881 atcgaagatg tcttccgtaa tggggacgta ttcccagttg ccgaaatact gattgcggaa
    14941 gtgattgaga ttgtttttct tctggcggta tttcttgccc gataagttga tcaggctctg
    15001 ggtcagatag acgtattcat aattgtcccg gtccggcgtg aagtcataac agtcttcaca
    15061 gagggccttg atacgttcga cagcttctgg tgtaacgcct ttcatcaaga aaggatagcc
    15121 atgttcatcg aaccattcgt gcatgcgcag gacgcctttg acgaaactgc cgtctttgcg
    15181 ggcgaagggc gggatccaga actgtttctt gccgcggcct gagcggatat agaggacatt
    15241 gtcttcttcg gcccactgga tttcataggg cttctgccag gcaaagagcg tcatgaaggt
    15301 agcgtcggcc tgttcatatc ttttctgatg aaaatagtca tctataagtg gtttatcttc
    15361 taagcgaaag tctctgaatt caataatggt aatctccccc taaaatattt acaccttaga
    15421 tacataagga ttgtcttgga tataaaaacg ccagggaaaa tatttcccat gtacggcgta
    15481 atcaatatgg atacgcggcg aaacggccac tgtaaacggg acctcttctt cgggatggga
    15541 aatatacagg caggaagacg tcaaatctaa agcgttttcc tcgcgcgtaa tggccatggc
    15601 ctgacagagc ttgcccggtc cattggtcag gttcctgccg gctttcctgg ccttccgccg
    15661 tgcggccatg aggtctgtcc cgcagatggg ttcgatggcc cgtatgagca cggcctggcc
    15721 ttcgccttcg ggaccggcga cgacattgaa gcagcagtac atgccgtaaa tcaagtaaac
    15781 atagctgata ccgccggcat ggaacatcgg tgccgtccgc ttggtcaggc cgcggaagct
    15841 gtgggcccca tcgtcgggaa agccgtcata agaaccgcca taggcttcgg tctcgacgat
    15901 acggccgctg cagatgccct gtggcgtttc atggatgaca tactggccca gcaggcgtgg
    15961 ggccagcagg ctggcatcga cacaaaactc gtcagctctc catctgcggc ctatcattct
    16021 ttcttgcggt agcctaagga atgggaaatt tcataggcat actgactcat agtaaacaac
    16081 atcttctggc ggatggaatc ataaaagcgc atagacggcc cggcaatggt caacgaggcg
    16141 atgacctggc cttcaaaatt gaagataggc acagacaggg cgaacagtcc atgctcgctt
    16201 tcatcctggg taatggcata gccgttatga cggatcgtag caaactcttc ttcagaaata
    16261 ttgccgtatt tttcgatata gcgctggcgt tcgtcatcgc tcgtccaggc cagcagcacc
    16321 cggccggcag cacccttata gaggggcagc gattcgccga tgggaatgac ccggcgcagg
    16381 gaatgggtac tttcgacgtg gtccaggcag atgcggcagt cgccttcggc aatgtaaata
    16441 ctgaccgttt cgttaaacaa ctgctgtaaa cgccgtaaat acggcttggc cagggtgatg
    16501 acgttttccg tccaatgaga cgaatagccc agcgccaggg cccgcggccc caggcggtac
    16561 tgcttggacg tatcgttgcg ggtcagatac tggcgccgtt ccaatgtgtt tacaatgcgg
    16621 aaagccgtcg acgtattcag atgggtatgc tcagcgattt ccttcaaggt catgcccgta
    16681 ttacattcga cgaagaggtc gaggacgtcg aggctctttt ccaaggattt caataaactg
    16741 ctgtgatgct cttcttttac caatggctct cacccttcct tggaattacg atttaacttt
    16801 catgccgatt tccagcgaat ccggcgggtc agcgacgacg atatccccct cgtcgagacc
    16861 atcgaggatg acgacgcggc cgtcttcttc atcgccagtc aggacagaac gggcatcagc
    16921 caggccgctg tcattgacaa tgtaaacgaa gtcaccgctg tcctttgtgc ggactgccgt
    16981 cttggggacg gtaatctggg agacttcttt cggcgtggca atgcggacgt gatagaattc
    17041 accgggaacg aacaggctct tggcgttatt gaatgtgacc ttggccatat aagagccagt
    17101 ggacggatct ttttccgttg ccagataggt cagttccccc gtctcggttt cgcccgaagc
    17161 ggcgatgatg gatacttgca ggctgccgtc ttttttggca tcctgtaaag cctggatata
    17221 catttcaggg atggcaaagg tagcaaccaa aggagacatc tgctgaatcg tagccaggac
    17281 attgccggca acggcaacgg acggattatc ggctacagcc gtgacccggc cgtcaagagg
    17341 cgaataaatc gtcgtattcc ccatcatatc gcgggcagcc tggacggcct gctgcagctg
    17401 ggcgatcttt tcgtggtcca cagccggagc ttcctggacc tggacggtct gcgtcgtttc
    17461 accttgagaa gctccagaag aactgccgga atcagccatc atctggttgt attcaacttt
    17521 cgataaggcc cccatattat accaggcctt caattcagca cggtgaccgc tgttgtcgcc
    17581 gccaccaccg ccgccactgc tggcagccgg tacggtaacg gtcttgtacg tcggcgtcga
    17641 tgccgaagaa gcagctacca gttccgcttc ggcctgattg acactctgct gatacggtgc
    17701 cgaatcgact tggacgacga cttcaccggc tttgacttcc tggcctacgg ccagcggtgt
    17761 cgatacgagt ttccccgata acttcggcac gatattggtc atatgtaagg ccgtaatgtt
    17821 ggcatccagg gtcagggcca cagggtggtc cgacttggaa accgtttcag ttttggtaga
    17881 cacgctgttg ccgcagcccg aaaggactga tacagccaag gcagccgtac aggccaggac
    17941 ggcccatttc tttaatttca cgatatcccc tcttcacgca ggttaaaaaa acgtgtaata
    18001 caaggtaacc agtatgaccc cgggcaggcc gaacaggccg atgatgaggc ctgtaccgac
    18061 cgtaatcgga acgtgctgga aaccaaaaag atagccaaaa tggttgacgc cgtacaggac
    18121 gatgagcccg acgacgacgt tgaccactaa atggcgcata ggcgccaaga tgagcttatg
    18181 cactaagaac aggatgaaaa tgcctgcggc tatccataaa tattccataa tcccctcaca
    18241 tttcctgccg attggcaaca gcaccagcca gggttgcttc atcagcatac tcaatgtcgc
    18301 cgcccatggg aaggccgcgg gcaatgcggg tcactcggat gccggccgtc ttgatgaggc
    18361 gggccagata taaagccgta gcttcgcctt ccacgtcggg atccgtcgcc aggatgactt
    18421 ccttgaccgt cgtattgccc aggcgctgca ggagctcgcg gatgcgcagg tcttcgacat
    18481 tgacgccatc caggggtgac aaggcaccgt gcaggacgtg gtacagtcca tgataatcgc
    18541 cgctgcgttc aatggcctgg acatcctgcg gcgtttcgac gacgcagatg gtcgagcggt
    18601 cgcggctgat gtcactgcaa atcgggcacg ggtcctggga cgacaagttg aagcagaccg
    18661 agcaaaaatg catattctgc ttggcatcgc ggatggcatc gacgaattca tcgacacggc
    18721 tgttgggctg ttgaacgata taataggcca gcttgcgggc cgtcttgacg ccgatgccgg
    18781 gaaggcggcg gaactgctcg gtcagcttat ctaatggcgt atccattaaa acatgcccgg
    18841 caggtgcatc ccgcccgtga ttttgctcat ttcctgagcc atcatgtcgt cgactttctt
    18901 gttggcttca ttgacggctg cgaccatgag gtcttccagc atttctgcat cttccgggtc
    18961 gatgatgctc gggtcgatgt ggacgctttc gacattcttt tcaccattca tgacgatagt
    19021 caggacgccg ccgccgacgg tagcttcgac ggtgcgcttc ttcaaatcat cctggagttt
    19081 cttcatttcc ttctgcattt tctgtacttt cttcatcata ccttgcatat ttccaccaaa
    19141 catgtgcgtt tcctcctaat tttcgtgtaa acttattttt tattttctat atatatatta
    19201 cagtctccta cgtttttaag caatggttcc agggcggctc tttccgcttc atccatatcg
    19261 tctaacgaag ccggctgcca cgccgtttca tcgcctggcc ctctggcttc ctgagccggc
    19321 gcaggagctg gaatatcttc ggtttgtact ggagctttgt ctacggcctt tacggggggc
    19381 aattcactct tttttttttc gtatcccgaa agttccgcat caccttccaa aaagccctgc
    19441 atgtggtacg tgccgcccag tatctgagat aaagcggcat cggtgaattt gaagtaatct
    19501 tcccgattgg cccgtttcac catgaaggcc gtcttaaagg cgacgatgac ctggccttcg
    19561 ccgatgtaaa cgacgcggcc attgcggata caggacaaga cggctttctt cttttccttg
    19621 tcgagaatgg cacacatctt tttccagacg tcctggtagg ctgccgactc gacggggggc
    19681 ggtgctgccg gaacaggcgg agttacctgc gctataatgt cggccggagc cgcggtctgt
    19741 acgacaggtg ccgccggggc tggaactgcg ggctcaacgg ggcgcaccgg ctgagggact
    19801 ggcgctgccg cttcccgggc tgccggacgg acggccggag ccggttcggg aggggtaaac
    19861 tcatcttccg ggaaggggac cgcttcttcc ggaccataag ccccgctgta atcgtcttct
    19921 tccggtacgg ggatgacgtc cggctttgtg gcagccgccg tcttggcttt tctccccgaa
    19981 gcctggggag ccacagccga tatactgacg gcaggcggct gggtcagctg ccgcgctgcc
    20041 atgagcaggc ccatttccac ggcaatgcgc ggaaccggcg acgtcttggc cgtcaataag
    20101 gcctgctgca aaatcgccag gacctgaaaa acactatctg ccgtccattc gccggcctgc
    20161 ctgcgaagtt catcgaggca gtcatcataa gctgaaagag tatccaacga aggcgcagcc
    20221 tgacagagca tgacagcccg cagatgggcc agcaattccg tcaggatgac ccgcggctcc
    20281 ttgcccatgt ggatgacgtc atcgacagcc ttgatgatga catcgccttt cccatcgaaa
    20341 atagcctggg tcagggaaaa cagccagtct ttgccgatga ggccgagtaa atcgcggacg
    20401 acgccttcgt cgatagtgcc gctcgtgttg ctgacgcact ggtcgagcat actcagggca
    20461 tcgcgcatgc cgccgtcggc ctggacggcc aggatatgag ccgcaccctt ggtcaggtcg
    20521 atctgttcct gaccggccac gtatagcagg cgcttggcaa tatcgtcgct ggtaatgcgc
    20581 cggaactcat aacgctgaca ccgcgacagg atggtcatgg gaatccgttc cggttcggtc
    20641 gtcgccagga tgaagatgac atgggccggc ggttcttcca aggtcttcag cagggcattg
    20701 aaagcttcgg tcgtcagcat gtgcacttca tcgatgatat aaattttctt atgcccttcg
    20761 gcaggcatga atttaatcgt ttcccgcagg gtccggatat cctcgatgct gcggttggaa
    20821 gcggcgtcga tttcgacgac atccagggat tcgccgctca tgatgtggcg gcagacttca
    20881 cattcgttgc acgggtggga cgtcggcccg tggacacagt tcatggcccg ggcaaaaatt
    20941 ttagccgtgc tcgtcttgcc cgtcccgcgg gggccggaga ataagtaagc atgggctact
    21001 ttgtccgtat ctatcgcttt ttgcagggtt tccgtaatgt gggactggcc gacgacatct
    21061 tcaaaattcc tgggcctcca cttacggtat aacgcaatat atgccatgag tccttttcct
    21121 tccgtatacg aaaaaaagca gctaaccgct gcttttacca actgaaaagt actcgtgtat
    21181 gataacccgc caggtttcct cgtggcacat ggaaatgtct acttattgct gcttcctctc
    21241 ggacctgaca aggttcatag attcccattg cacaagcccc ggcggattac cacgcaccag
    21301 tactcttatt aagttagcta cattattata tcgtgaaaaa cgaagtttgg caaggaaaat
    21361 ttcccttttt gagagaattt agtggttagg ggctaggagc taggggtgta gtggttagtg
    21421 gttagtagtt agtggttagt agttagtggt tcgtggttag tggcttgtag ggggctccac
    21481 gtgcgaaccc ctacagaaaa aaaggacccc tcgcacgaaa gcttctacca tcatgcgagg
    21541 ggcttttttg gtcaaaaaca acctctcaga ccgccttcgg cggccagctc ccctataagg
    21601 ggagccgcta gccacgaaca actagccacg agccactatc ttagtgattc tgccagatat
    21661 ctacgcggcg gttgtcagcg cggccctggt cggtgtcgtt ggtgctgacg ggatctgtct
    21721 tgcctttgta gtcgagctgc atctgtgttg ccgggatgcc ctggctttca gcataggctg
    21781 caacgttgtc tacgcggcgt ttcgagaggt catcgttgta agcatcgctg cctttagcat
    21841 cggtgttgcc gacgagtttg aacgtatcgt tcgtgtttgc tttagcaacc tggacgaagt
    21901 ggtccatctt agcgcgttcg gaaggaagcg gctggtcttc gtcgaagccg aagtgaacgc
    21961 tttcaacgta gtagtcgttc ttctggaccg gcgtttcaac gaccggtgcc ggagtcggtt
    22021 ccggcgtgta aaccggttcc ggagccggag cttctttgtg gccgccgccg aagcgatagg
    22081 agaggccagc gatgaagcct ttgtaggaga tgttagcgtc gtctttcaat gcacccaagg
    22141 tgttgtcgcc ttcatcagcc aatttggtgt tcaagtaacg atagccagcg ctgatgtcga
    22201 ggtccggtgt gatgctgtag ccgaggccag cttcccacat ggtcgtcttc ttggtaccga
    22261 cagcgccttt agcgtagaag tcgaggttct tagccagcgg agctttggcg atcaagccga
    22321 tctgagcgac gttgttggtg tcgttagcat cgccaccgag atcgttttta atgcggttcc
    22381 agccggcgta agcggcgaag ttcttgttga tggagtaaac gaggtttact tcgtgttcat
    22441 cgccatcggt cttgtaagac ttgttgttgt cgctcttcgt cttcaagccg tagtagttat
    22501 actgcaaagc ccatttgtcg gaaaggccgt agccgaggcc gccctggaag tcccatttgg
    22561 aatcagtgtc ttcctgacca cctttccatt cgtctttggc ccagttgctg gaatcaaccg
    22621 aagctttcgg gttccaagcg cctaaatcga gctgccactg tccctggttc cactgcgtct
    22681 gcggcgtagc gaaagcaaag gaagtagcgc aggctgccat gactgcagcc agtactaaag
    22741 atttcttttt catcataatc ccagcttcct attgatattt aatagtgcca cctatgacat
    22801 tattatgttc tagtatacca tatctttgtc acaagtgcgt cacactattt catgattttt
    22861 agtcatatca ggactattcc ccatactttt atgattattc ccggccgacg taagaaatga
    22921 tggcgtccag gatatgcggc aatttccggc tcacactgtc gatatggacg cgttccgtcc
    22981 cttcgtgcag gtccttaccc catgggccca ggaggaggaa gggaatctgt aattttttca
    23041 gactgtccaa gtcgaaggga taggccttgc cccagaggag caggttcttt tcgaccatac
    23101 tttcatcgaa atcggggtcg acgcagcagt agctgcaatc ggaaacaccg ttgaaatagc
    23161 ggtcgaagcg gacatccatc agagtcggga ttttttccag caggtccgta aaggccggcg
    23221 tcgggaacga caggctgtca gccgccggat aacagggcgg cgcaaaggcg acgacggcca
    23281 tgggcgccgt catgcctgtg aaatcgagga tctgctccaa gagatgtatc gtctcttcga
    23341 cataagtact gctgccttcc tgcaaggcga cgaaagaagc ctgtttggcc gcttcataga
    23401 agacgtcgaa accgggataa gccgaagcct gctgcaagag ctcatcggca ctgcagacgg
    23461 aaacaggcaa atcactgccc atggtctgct gcaggtcgtg gacagcccgc cgcgtttctt
    23521 ccagcaggac gtacatgacg tcatcgggcg tcttgtcgta agtcagcaga ttcagggccg
    23581 ctgccacccg ctgaggcagg gtcacgtcat agcgttcctt gcggtcccgc agatagaccc
    23641 aggcaggcgg cggcgtctgt tcgccatcac agcagtcagt caggtccgga ctgccttccg
    23701 tcgccgcgat gaggtgggcc aggatgccca cgggattgat gcccttatca tagcagccga
    23761 tgtggacagg cttgccctgg accaggacga caggctgtat cttgccgacc gaaccggtat
    23821 aggcgacgag cttgccctgc tccttatggt tcggttcgct gtcgatgagg atgtcatagg
    23881 tcaaatcata agaagaacgc agttcattga gcaaggtaat ggccgtccgc atgccggccg
    23941 aatacgtttc ttcatcgggc agggataagt aaatcaagct ggcctgtccc ggctggaagg
    24001 catagtcctc aaagacggct agttgcgcgg ccgcaccgcc cttcatgtca caggcaccgc
    24061 ggccaaagag ccattcgccg ctttcccagt cacggcgcac ttcctcactg agcggcgccc
    24121 gttccagcgc cttgtagagc ccttgcgggt caaaagcctg gtcccgcaga tagccatagg
    24181 actcgacatc gacgacatcg tgatgattca tgaaaatgac cgtatgggcc gtactgccgc
    24241 ggacgaggcc gtagacgaca gaccgctggc agttatcgtc gggcacatgg atgaggccgc
    24301 tgacgacgcc gtcgaggtga gagaagaact gctgcagata gccttccatc tgcctttcct
    24361 tgagcgtgtt cgtttcactg tcaatatgaa tcatatcctg taaaatttcc ataatccgcg
    24421 ctgctcgtgc catagagaca cccccttatt taaatatcca ttttttacgc gatacataat
    24481 tccaaacggt gacgatggca ctagccatga ttttcgacac catcggattc aggcctaaaa
    24541 tagccggcga tgtgaacagc cacatgagca ggagattcca gcccaggcca atggcactga
    24601 ccaggaagac cagggacact tccgtcccgc gatgatagcg gctgccgctg tcgaagacga
    24661 agcggttgca gaggatgtag tgatacagcg tcgacgtggc aaaggccagg gtcgtcgccg
    24721 ccagaacagt ctgggtctgc atggaaaaga cagcactggc tgacaacagc ctgaagaaaa
    24781 tataaaagag cgtccattcc aataaagccg ctgagccgcc gacgaggaga tacatgaaga
    24841 tcttcatgta ctcgttgttg gtataatcaa agccaaaaat ctgctttcgt ttcatggtgt
    24901 tccttccttt tctgttttcg tatccattat acggcacttg ccagggaatt accacacact
    24961 gccgcttttt cttgcatttt ctaaaaagac aatagatata tatacgaaaa tatgataaaa
    25021 tatatagtaa ttggtaatac gaaatatttt tagaaggaag gcagatatcc tgtggattac
    25081 acgagatgca tcaatttttt gctaaccatt tcacaacacg aagtcttttt gattttctcc
    25141 gagaacttgt cggaattcgg catcacgccc ggccagtacg gcgtcttagc ctgtctctgg
    25201 aaacatgaaa cactgacgcc gaaggaaatc gccaacatcc tgcgcgtcga aaactccacc
    25261 atttccggcg tcctcgaccg catgcagaaa cgcggcctca tcgaccgcat cctcgacccc
    25321 aacaaccgcc gcagcatccg cgtcaaaccg acggctgccg gcatggccat caaagaaccg
    25381 gtccagaaac gcatcgaaga actgaatgac atggtcttca aagaattcac accccaagaa
    25441 cgggaagaag tgttgaacct gctcacccgt atcggcaaag tctgtgccga tgatttgaaa
    25501 gaagggaaat gaaaaagggg ctgtcgcacg tgcgacagcc ccttttgagt tttaagtttg
    25561 tagtttgtag tttttagtga aggtttttaa atttaaagtc cctatctaca aactctatac
    25621 taaaaactat aaagcaaaag ggaccgcgca tgatgacata taccttcatg cgctagtccc
    25681 accaaattat acaataccag ttaacatgta gaagaatacg ccgaagaagg cgacgactac
    25741 tttcaagagg gtaatggcga acatatcctt ataggcttcg cgatgagtca ggccgcagat
    25801 agccagggtc gtgatgacag ctccgttatg gggcaggctg tccataccgc cggaagccat
    25861 ggccgcgata cggtggacga cttcagccgg gatgccgacg gcagccgttt cagccaggaa
    25921 gtgctggcca aagatatcga gggcgatact cagaccaccg gaagcggaac cggtgatacc
    25981 agccaggatg ttgaccatca aggcgacgtt gaacagcggc atgccctgac cgataccgag
    26041 gagggcatca gcgacttcca ggaagcccgg caaggacgaa atgacgttgc cgtagccaac
    26101 ttccgatgcc gtgttcatga tagccagcag agaacctaaa gcgccggcat tgagagcggc
    26161 tttgacgctg gtgttcttgg acatgaactt gcggcccgtt gcagcggcga tgatgatagc
    26221 cgccaagagg ccgaggacca gggcccagat agcgacgacg ttgtggacgc ctttggccag
    26281 gagaggcaca ttcatggcgc ggaacggttc cagtaaggca tcatcccact ggaaaatcat
    26341 ggagaagtag aagttcaaga ccaggacgac gatgagcggg atgatggaca actgccaggg
    26401 cggaagagaa gcttccggat cgatgaccgt ttcattgagg gtatggttgc catagccttc
    26461 gccggaacgg gccatctgtt tgcggcggaa ttcaagataa atgaagcaga ggataatcag
    26521 caaggtaccg caaatcaagc ctaagatagg agcggcaaaa gtcgtagttc ccagataaat
    26581 cgtcgggatg atgttctgga tctgcggcga accaggatag cagtccatgg tagccgtgaa
    26641 gacaccggcg acgaagcagg ccggaatcag gcgtttagga atatcggctt cgcggaacag
    26701 tgcagcagcg aagggataga cggcgaaagc acagacgaac atggaaatac caccataaca
    26761 gaggatcatg caggcgaaga cgacggcgaa gacagcctta tctttcccga tgatcttgat
    26821 ggtttccatg gcaatactgc gggccatgcc cgtttcttcc atgaccttgc cgaagacggc
    26881 accgaggagg aagaccggga agaaggactt aatgtaggta acggccttag ccatgaacag
    26941 ttccgtatac cccggtaaaa tcggcagccc cgaaaagctg gcggcaaaga gggcgaaaat
    27001 cggtgcgaag agaatgaccg agaatccgcg ataagcaaat gttacgagca aaaataaact
    27061 taggacgatt gcaacaatat ccatatacgt gttctccttt tcttttggtt tccgaatgac
    27121 tcatttcgta tcttcatcat acgtgagaac caatgagaat aaaagacaac gccctaagac
    27181 aacctttgcg ctttttgccc gttttggaaa ttccatgaat gcaaaaggac ttgccgcaca
    27241 tgcgacaagt cccttttgag tggttagtgg ctagttgtta gtggctagcg gatggttaaa
    27301 aatttaaaaa ctgggattat ttcagtttag cagcgacgtc tttggtgatg tcctgggact
    27361 ggccgatgac gacggagccc gagaggacga cgtagtccag gcctttttct ttctggacgg
    27421 cctgggtctt agcgaggata tccttttcca agggcgagaa cagggcaatg cgctgctggt
    27481 tcagtttcgt attgtatttc tgtaccagtg cctgctgcgc gtttttatcg ctggtctttt
    27541 tcaattcttt ctggaaatcc tgctgagccg atttgacatc ggactgccaa gtagccatgg
    27601 tcttcgcgta ctgcggatgc tgctgcaaga gggcaccggc attgatcgtg cccaggcccg
    27661 atgcgaaaga agcgccggca gcgctgaagg tcagcatagc tgcggctacg gtggtcatca
    27721 tcattttacg taatttcata gtggttttcc tcctaattat tcttctacgc ctgtcagttc
    27781 cagacattcc ttgcggacgc cgtcttcaca agagcgcatg gctaaaatcg tcttttcaaa
    27841 gaggtcgttc aggtcccatc ccagggtttc tgccccctgc ttgatgacgt cccgtgaaca
    27901 gccggcagcg aagcgcttgt ctttgaattt cttcttgaga ctcttcactt ccatgtcttt
    27961 cgtgctctta gacggacgca ttttggcagc cgcccagatg atacatgtca attagtacgc
    28021 agcatatagg gctttatcca tcaagtccac ctttgcgaaa t
//
LOCUS       DAWCYS010000005        24361 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_115_length_24361_cov_8.753765, whole genome shotgun sequence.
ACCESSION   DAWCYS010000005 DAWCYS010000000
VERSION     DAWCYS010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 24361)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24361)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24361
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115_length_24361_cov_8.753765"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5793 a         6646 c         6285 g         5637 t
ORIGIN      
        1 agtgctcgac gactgtcgat atcggattgt attcggtttt tttgctagct tagagtgctt
       61 cctgtttggt ctagttctgt gtaatagtcg gcagcgtcgg tttctgctgg aagtggaagt
      121 tttcgtaata ttccggatgg ttggcatact ggatggccag ggtgacatcg ggatcaggat
      181 tatccgggaa gccatcgtgg ttggcatctt tgaaggtcgg gaagatggag tcggcataag
      241 tgcggacagc tgccaggccc ttcttgccat cccgttcatg gtaggtcccg gtaatggaag
      301 cgccgtgagc atggtcggcg acgacgatga tcagggtgtt gtccccgtgt tctttattcc
      361 atttctgggc gtattcgatg gccttgtcga attcgatggt gtcatacgtt gcgcgctgcc
      421 aatccatggc gtggagctgt ttgtcgatgg aagcgccttc aatcatggca aagaagcctt
      481 tcgggttctt gctgaggata tccagggatt tcttggtcat gttaatgagg ttcggctggt
      541 ccgtatattt gccgaggact tccgggttct tgagtatttc acggtcgaca tagacattca
      601 tcgtgctcgg atggaacagg cccaggagcg gtttattatc cggagcggcc atcatttcct
      661 tagaattgct gacaaaggta tagcctttat ccttgaattc ctggatgacg ttctggttgt
      721 catggcgctt cgagccctgt tcggactgcg ggatgtaacg gcgcgaaccg ccgcccatga
      781 tgacgtccgg gcgatgataa tcttcgaggt attccgaagc gatgtaatcc tggttggcac
      841 ggcggcgcgt atgaccgacc atggctgccg gcgtggcgtc tgtcgattcg gcctgggtga
      901 tgataccgac agacatgccg cggctgcgtt ttacgatttc cgaaatattt tcgacgcgcg
      961 gatcatcgaa ggggtctttc gtggagtctt catagacgcc caaggcgttg acgacggatt
     1021 tatgacctgt tgcataggta gacgccgagt tggcactgtc tgtcgtcagg gaatcgtaac
     1081 cgctggtcgt aatcaggcag ttatggggca ttttttccat ggccagtaag tcattgtact
     1141 tgccgttagt catgcctttg gaaataatac gggccatttc gcgggcctgc agggacatgc
     1201 cgtcgccgac gaagaggatg acgttcttgg cctggcgctg agctttgtcc tgaacgacgg
     1261 tatagcgggc atcggccgaa ttagcgccat cggcatcggt agccgtcgct tttaccgtat
     1321 aggcaccggc tttggggaaa ttgacctgat tggcacgata tgaaatgacg ccgttgccta
     1381 aatctttagc atctaatttc tgaccaaaga atttatcagc cttctgtcca ttgaccgtaa
     1441 tgtcgacatt cttcagattc tttgctttcg atacttcgat gtcgaaatcg aatttggcac
     1501 cagccaggaa tttcgcatta tcgacgggaa ggatgcggac atctgccgcc tgtactccgg
     1561 gaatgaatcc tgctaatgag ccgcaggcta ccatggaaag aattgctcgt tttaaccatt
     1621 gtcgtttcat atcgtgacct cctgaatttt gtaacatatt cgacacgata agtataaagt
     1681 ccctatacat agaaaaggtc aaatctatgt ataatttatg tataatctca ggaaggagtg
     1741 tatactcatg atttacagct gcatagatat cggtggtaca gccatcaaat tcggcctcat
     1801 cgacgaaacg ggccggttcc ataagaaagg cagcctgccg acagaagcgg cgacgggcgg
     1861 cggtccgggc attgtccaca aagtccgctc gctcatccag cgccagctgg ccgaccatcc
     1921 cttagcgggc atcggcatct cgacagccgg catcatcgac acggaaagag gggccgtcga
     1981 ctacgccctg tccatcccgg actatacggg gacggcctgg aaatcgctct tggaaaatga
     2041 attccagctc ccctgtatcg tcgaaaacga cacgaactgc gccgccctgg gagaatggtg
     2101 gctcggcgcc ggccgggaca tggactccct cttcgccgtg accgtcggca ccagtatcgg
     2161 cggggccttc atcgaaaagg gccatatcct tcacggagcc tgccggtctg ccggggaaat
     2221 cgcctatatg cgcgtccctg ccggccgcct ccacgacgtc gcttcggcga catacctctg
     2281 ccgggaaatg gcatgcaaaa aagccgccga cccggaaaag gtcgacggcc gtacttgctt
     2341 tgcctgggtc cgtcagggag atgccacggc agcagctgtc ctcgatacat tctgccagaa
     2401 tctggccgat ggcctggcca atatcgcctg cctgtgcaat cccgatggca tcgtcctcgg
     2461 cggcggcatc atggcccaag aggatatcct gaggccgaag ctggaaacag ccctggcggc
     2521 gcgattgccg gccatgatgc tgccgcctaa gagactggcc ttcgccaaac ggcagaacga
     2581 cgccggtatg ctgggcgcgt tgtatctatt gctgcatcga aaaggcagct attgatgatt
     2641 cagtttcgtg cggacgatgc gggttacttt ttcatcgact aaggcccgga attccgcgtt
     2701 gctcttatat tccttcaaga ggccgtcata cgtttcccgt tcgtgggcat attcatggca
     2761 gaccaggacc atatcggcac cggccttgat ggccatgacg cccatgtcgg caaaggcata
     2821 gtgcttggcc atggcgccca tttccatatc gtcggaaacg atgagtccct ggtagccgaa
     2881 agtgttgcgc aataaatcgg tcatgattgg cttggagaca caggccggaa gattggggtc
     2941 cagcgccggg aaggtgacgt tcgagaccat gatgaacatt ttctgattgt cttcgctctt
     3001 gatgaggttt tcaaaaggct tcaaatcctg cttcatcaag tcatccttgg aactgttgac
     3061 gctgtcgccg tcgatatggg gatccgtctt gactttgccg atgcccggga aatgcttgag
     3121 ggaacaccag attcccgtct gttcgtagcc ggcaatggct tcatgggcga attgggtcac
     3181 gacgccaggg tcgaggctgt acgaccgttc agcctgagaa ccgaggtcga cgacaggcgc
     3241 aaagttgacg ttgatgccca gctttttcag ggcatcgccc gtcgtgacgg cccactgttt
     3301 cgcctgggcc gactggccgc tctggccgat ggactgttca gacggtacgg gcgggaaggc
     3361 cttgcgcatg cgcaggacct ggcccccttc ctgatcgagg gcaatgaaag gcatgacgcc
     3421 gctcttcttg cggatcgtcg tgtctatttt ttttgtcaaa tgctggacct gttcgggtgt
     3481 atccatattg cggtcgaata agataacatt cccggcatgg cagtattcca gctggcgctg
     3541 gccctggtcg tcgatggtcg tcccggccag gccgatcatc atgagctggc cgatttttac
     3601 ttccggtgac atcttggcga cgagccgcga tgcttcatct tcctgaacag cgcctttaga
     3661 gggccctgcc gggcccgctg gcgacggcga acggtcccag aagaaccagg cgccgatggc
     3721 tgccagcacc aggatagcca gtgccaggaa aatgccgctc ttcgtattag gcttcttcat
     3781 tatatgccag ctccccggcg atgaccgtct gttttacatg gaaatcgttg ctgtccagga
     3841 tggtaatgtc cgcctgcttc ccttcttcca gagaaccgat gcggtcgtag acaccgaggg
     3901 ctttggccgg gttgaccgta gccatggcga cgacatcggg aatggaggcg ccggaattga
     3961 tgaggaaatg gaggaccact tcgttcatgg gggcaatact ggcagccagg gtcccgtcag
     4021 ccaggcgggc ttcaccattc ttgacgaaga ctttctggcc acccaagctg gattcgccat
     4081 cgccctgcag gcaggcccgc aacgagtcgg tgaccaggat gaggccgtcg cggcctttca
     4141 tctgataggc cagtttctgc gctgccggat ggacgtgcaa atcgtcgcag atcaattcac
     4201 agcaggcgcc cttatggagc agggccgcgc cgacgacacc gggtctgcga tggtggaacg
     4261 gcgtcatagc attatataaa tgcgtaacat ggcagctgcc gagggcgtcc atgcagtcca
     4321 tggcctggtc gaaatcggcc gccgtgtgcc cgatggacac gataataccg tggtcctggc
     4381 agtccttcag gaatttcttg ttcttcaatg tttccggtgc caaggtgatg atcttgatga
     4441 cgtcggcata tggttcgagc caggaaaaat cggcagtctg gatattttct tccgcctggg
     4501 agcctttgta ggccgggctg atgaaaggcc cttccatatg agcgcccaat acctgggcgc
     4561 cgccctgata gtcgtcccgg gcctgacgga tccggctgag ggctttctcg atgcgtccct
     4621 gttccatagt catcgtcgtc ggtacgaagg acgtaacgcc cgtcgccggc agggcctgct
     4681 gcatggtctt cagggcctgg tcatcgtcat ccatagtgtc ggcaccggca cagccgtgga
     4741 tgtgttcatt gatgaatccc ggtacgacga agccgccatt ggcatcaatg aattccgagc
     4801 acatgccggc ccggaactgc ttgcgcggta cgatgcgcca gatatccttt tcatacataa
     4861 tgacgtgacc gttaatgatt tcgttcggca ggacaatgag tccgtcgatg atagctttca
     4921 tgttgatttc ctcctaattg tgttcattgc ctccagtata ccacaaacgg caaggctgtg
     4981 gaaattgtgg ataaatgaag tggataagtt gtgtacacac tgtcagaatg gctattttat
     5041 gctttgtcca cctgtgtata cctttccaca atgtatccac atattgtgca taacttgccg
     5101 agaacattta tccacatttt ctcaggaatt atgcacatta tctgcgccat ttgttcgtcc
     5161 attataaatc gcgtaatcat cccgttttca ggccgttttc accggaggta cacatttatc
     5221 cacgaaagtt atccacattt cccctgcgaa tccccaaaaa gtatgcacct gaaaaaatca
     5281 gcataaccgt gcgcttcaca aaaaaaatat ccacatttct gtcacccttg tgcacatgaa
     5341 atgtggataa attgttcata gattatggtg gaggcttatc ccttaatcga cagggcggct
     5401 ccagtcaatg tggatgcccg tagcccgcca gatatagcac aaggccagtt tcagcatttc
     5461 caggctcgat gtcgggacgg tgctgatggt cttatactgc gctaaatccg tatactggtg
     5521 caagactttt tctgtcgtca aatactggcg ggcccgttca ttcatgacgt cgtcgttcaa
     5581 gtggacgccg agttctttcg tctctgtatc gtattcgatg tacccgttgt tgttatcacc
     5641 cgtgtgggca gctactttaa aaattgccat aacaaaaact ccttctttat cgttcttatg
     5701 tatctttttc atgggacatt tcccaaagaa atcctttcat gatggccgtg cggccaaagc
     5761 gtttctgcag cttgtcgatg gcttccgtca ctttcgcctt cgtctcttca tcttccgaga
     5821 acaagtcgcc ctgcacctga taggcctgga gctggctgac cgtcaggccc aggaggcgga
     5881 tgggctcatg acctctgctc ttattataca atttttttgc cgaaaaatat aactgttctt
     5941 ccgaacaggt ccctatgctg tgcagcgtca aagagcgggt aatggtcttg aaagaggtat
     6001 agcgtatctt gatggtgatg atccgtccca tgagccggtt ctgccgcagc cgccacgaca
     6061 cttcgttggc caggaggcgg aactgttcat ccacttcggc gtcgcgcgtc aggtcatggc
     6121 tgtacgtatg ctcattgccg acagactggg gccgccgccg tacttccagc ggccggttat
     6181 cctggcccag ggacaagcgg taaatccgct gggcctgatt gcctacgatg ggctggagcg
     6241 tttcgggact ggccgcggcg atgtcgccga tagtatgata gcccgctttt tgcagggcct
     6301 cttcggtcac cttgccgacg ccccacaggc ggctcagcgg caagggtgcc aatatctgcc
     6361 gctcagtccc atagggaatc cagaccaggc catccggttt gcgaaggtcc gatgccagtt
     6421 tggccaggaa cttattcggt gctatcccag ccgatgcgac gaggcccgtc gtccgccgta
     6481 tttcttcctt tacggcttta cccatgtcga cgatatccgg ataatggcgc tccatgccgg
     6541 agacgtcgag gaaggcctca tccagggcca gcggttcgat gaagggcgag taatgggaca
     6601 ggatcaggcg tatctgatgg gaaatacgcc ggtaatggga catgcgcaca ggcaggaaga
     6661 cgccattcgg acagagcctg cgggccagtt tcatgctcat ggccgaatgg acgccataag
     6721 ccctggcttc ataagacgcc gtcgcgacga cgccccgctc catcaggccg ccgacgatga
     6781 ccggcaggcc cttcagggcc ggattatccc gttgttctac agaagcataa aacgcatcca
     6841 tatcgacatg catgatccat cgccgtgcca taaccatccc tcctccattc tactttatat
     6901 tatacaacga ttggccgccc taaatataga ggggcgcatt aattcgtaca aattattgtt
     6961 aacaattaga cagtctacag aaacacagat tgctttaaaa tggcttagta ccgccattta
     7021 taaagaagta tacatcattg gattttcatt ccgtaatatg gtaaactata agaatactaa
     7081 agagaggtga aacattatat catggattta ttccaactga catacttcat cgaagtcgct
     7141 cacaaaaaaa gctttaccaa agcttcaaaa tcgctgcaca tcagccagcc gtcgatcagc
     7201 aaggggatca aagccctgga agaacactgg ggtgtccagc tcttcgaccg caaggggaaa
     7261 aatgtagagc ttacggaaac gggttcctat ttgcttccta aaatcgaaga actcatcaag
     7321 ggattcaccc agctgaatga agaaatggag tcgccacagc tgctcaatgc cggtaaattg
     7381 tccgtcggcg taccgcctat gatcggctct tccgtcatcg cgccgttcat cgcccatttc
     7441 atcaacaaat atcccaaaat cgaactggaa atgaaggaag tcggttccca agacgtcgta
     7501 ttcgccattg atgacggcct catccaggtc gggttcgtcg ccctgccgat tacgacagaa
     7561 attccttatg aatttttcat cttccacgaa gaacctctcg acgtcgttct ctggccggat
     7621 caccccctgg cctcccagcc atcgctgacc ttgtcggata tcaaagacga aacgttcgtc
     7681 ttctacccaa gcggattctc cctgaatccc tacctgcgtt cggctttcca ggaaatcatg
     7741 gcccatccga agatcgtctg ccgttccagc aactgggact tcctggccca gatggttgcc
     7801 ggccgcctgg ggatttccct aatgccgcac acgatctgtc agcggatttc accggacctt
     7861 gccgtctccg tccccctcgt ccagccccat ctctattgga ccctggccct gatctggaaa
     7921 agcaagggct atttatcgca cccatcccgg acatgggtac agtcgtttaa agattatttc
     7981 aaggatgcca acgcggccat ccagagtgcc gcagccgaac atccttacct ttcagaggag
     8041 tagatcacga tgtccacatc attgctatcg gcagcagaaa aagacgaaat tttttcacat
     8101 cagcatgata acggtccctt cagcgccctg gccctggaaa cggcctgcct gaactatctg
     8161 gaccggctga accgtacctt ccgcaatccc ctggcagccc cggccgaccg ccgggcagcc
     8221 cttaaaaagg ggatggccct ggaagagaaa ctgttcgagt atatccgcca cgagacgccg
     8281 atcagctatt ttgacagcga tttccgcaaa cagacgaaac agtatatccg catgcgggaa
     8341 atctatgtcg acgccgtgaa cttcaccttc aagcgccacc gcttctgctt cgtcctggac
     8401 ctgctgcgcc tgtacagcga agatccgtgc cagattctcc ctgaacggga tatctttaag
     8461 gcaaaatggg aacaagtcct gctctacgac tacctgctcc tcgacatggg ccagaagaac
     8521 acggaagaca tcggccgcga agccgtatcc aacggctatc acgaatgtga ctataccctg
     8581 gaaatcgaag aagtatggaa acagccgatg aaagccgttc cccgctccca cttccgctac
     8641 gtcaaagccg ccttgcccta tagccagggc gcccgggcca tcgcaacgtg gatgaaagac
     8701 cacgccgccg aactagcacc ggccctctgg gtcgtcgata cccaggccat cgaagccctg
     8761 cgccaaggac cggacctgac cgtcactgat gaagatatcg cgattattga gaaaacattt
     8821 taaaaaaagg cttgttgcat gtgcgacaag cctttttttc gtaggggccg tcccaaaggg
     8881 cggcccgttg tagctggctg ttagcagtca gtggatggtt tcaaatttaa aagccatcac
     8941 attaaatggt gtttcaaggc gtagtctacg agttcggctt tggtcttgca gttcaatttc
     9001 tgcatgagtc tcgtcttata agtcgacacg gtcttgaccg agaggtgcag tgtgtcgcca
     9061 atggccgaca gggaatggcc gtggaccagg gccacgagga cttcctgctc ccgcgccgac
     9121 agggccggat gctggccggc accatcgagg acgccatcga ggaggctccg ggcttcgcgt
     9181 tcactcaggt agcggtgtcc ggaaacgaca gagcgcaacc ctttgaaaag ttccgcatcg
     9241 agcgtgtcct tgcagagata gccgtctgca ccgagctgca tgagttccgt gacgtactgc
     9301 tgctcactgt acatggtcag gaccaggact ttgacatcat aatggcggcg ccggatttcc
     9361 cgcagacagt cgatgccatt catgaccggc atggaaaggt ccagcaggac gacgtcgaca
     9421 ggcgtgtctt ccagccgctc caggacttcc tgtccgttcg aggcttcggc gacgacatgg
     9481 cagtcgtcct gagaggccag caatagtttc aaccccgtcc gcaagatacg gtggtcatca
     9541 gccaagagaa tgttcatgtt cttcctcctc actcaaatac aacggcagag aaatggtcag
     9601 cgtcgtccct ttaccaggat ggctgtcgat ggcaaaggtc ccgttcaata catctgtccg
     9661 ctcgcgcata ctgaccagtc ctaaatggtt atccaggcga gcccgttcgg cccggtccgc
     9721 actgaagccg atgccatcgt cgcggacaga caagttcagc ttcgacggca ggacgtggag
     9781 cccgatggtc acggtatggg cctgggcatg acgggcaatg ttggtcagcc cctcctggac
     9841 catgcgatag caaaacaagg ccactggccg ggacacgcgc gatatgtcgc cgtcatgatg
     9901 gaagatcacg gtcagccccg gctgtgtctg gcgatacgtg tccaggtact tttcaatggc
     9961 tacgaccagg cccaggtcat ccagcagggg cggatggaga ttgacggcga gatggcgcag
    10021 gccttccaaa gtctcgcggg tcaaatcgcg gacaccggcg acgagttccc ggacctttgg
    10081 ctcttctgtc tggtccatga tgagccgcaa ataggccagc atcgatacca tggactggct
    10141 cgtctcatcg tgcagttccc gcgaaatgcg gcgccgttcg tcttcacggg cagaaaagag
    10201 ctggtcgatg agccagatac gggtcttctc cttttcccgc agggcggtca gcaatttcga
    10261 gttctcccgg tcttttacag ccaggcggtt gctcatgaca ttgaaggctt tcgccaggcg
    10321 cccgacttca tctttcgtct cgacgggcac gcggacacta taattcccgc ggccgatttg
    10381 gcggatggcc tgggccaggg tctggacagg ccgcaaaaaa gcccgggcat agcgcgtagc
    10441 cagtacggaa gctgccagac agatggccag gatgaccagc atggtctgga cgaagcgctt
    10501 acacatgagc tgcatcatcg gccgttccga caagccgatg cggaaatagc cgaccaggcc
    10561 gccatcaatg ggatagacgg cttcccgtat atatccttcc gtactgtcga aggtcacagt
    10621 cttatcggct ccgtcgtgct gcacgtcgcg ccgttccggc aagccctttg gcaggccgtc
    10681 cgtaaacgtg ctggccagga tacggccatc gggataagtc accaggatat accgcacctg
    10741 gtcattcgtc tgctgagcat ggagcagctg ttccgtcatg gcaaagcggt catcgacgag
    10801 gatatcgtta ctgatgacct gactgattga gactgccagt tcccgcccgg atgtatcgag
    10861 ttcgtaattc atataggtgc tcgtcgagta aaggaacagg gaagcagcca gcaggccgca
    10921 gacaaagatt gtgccgacga tgagggcgtt aaattttaca tagagtgtat agtttctcat
    10981 atcaagtccc tgccaatcgt ctctgttccg catactgccg cttgaactgc tcataagccg
    11041 ccgtatcggg agctacgaag cggtcgatga caatagtctt catggaccgc gataaatcgg
    11101 ggttttcgtg catcgtatag aatatccgcc gcaaggacgc cttttcttcc gagtccaagc
    11161 tgctgcgcac gacgataggc cctgtcggcg cttccggcat cttttccagc acccgcacct
    11221 gccgggccag ctggggattc gtctgctcta tgagctcata aatctggctg tccacgccag
    11281 ccgcgtcggc caagtgattg gccacggccc agatggactt gtcatggccg tgagtgaaga
    11341 agaagcgctt aaagaactgc tctggcgtcg tcccctgctg ggccaggcgg aaagtaatgg
    11401 ccaggcagcc agaatagctc aaggggtccg taaaagcaaa gacccgcccc tggagctggt
    11461 gtttatcggt gatgtcgctg tctgcggccg taatgatgta cgtctgataa tacgacgagc
    11521 cattcgtttc ggtcatggcc agcagttcaa tgggctcatg accttgataa gccgcatagg
    11581 ccccagtcga agaaaaggcc acgtcggcat cgccattggc cagcagcgta ttcagttctt
    11641 cataggtccg gcgctggatg aggatggccg gccggttttc ctgctgcgaa atataattga
    11701 cgatctgctg gtatacctgc cgcgtttcct tcggcgacat gaccgacgca aaggcgatgc
    11761 gcagcggctt cgtatccggt tcctctgcag ccggatgcgt atcctgaggc tgggtgaagt
    11821 cgatatagcc ctgcggacgg gcacaggctg cgcagaacaa agaaagcatg accagcacga
    11881 tcagtgaaag gacccgtttc atctgttacc tccataaatc attacgttta ctatatcata
    11941 caggatggag atgcgcaaaa agaggttgtc acattaagac aacctctttc aaattaacga
    12001 atcaatcttc tttcggtttg gcgttccata cgaggagggc caatacggtc gaagcgatgg
    12061 cggcaccgac ccagaattcc gtagccatgc cgaaatcata ggtcttgacg ccgttgacga
    12121 cggtaatcgt catgagcttc ccagtgatga attcctggat agctgcaccg gcataactga
    12181 gcaggccgat catgcccatg gctgccccgg tgacgcgctt cggtacgata tcgacagcca
    12241 tgaggccgcc catgtagcag accagggcgc ccatcgtgaa accgtagaag gtcatgcaca
    12301 gcatatccat ggtgaacgac tgcggtgccc agaggaaacc ggcaatcgac agggagtaga
    12361 aaatcccgta cagcaaggcc ggcatgttgc gcttgtggtt aaagaatttg tcagaaatga
    12421 cgccgcacaa cagagccccg aagataccgg ctacctgcat gtaagccatg acgccggcag
    12481 cgcccatgat gtcatagccc ttctgcgagg tcagatagac gacgccccag ctttcgatgg
    12541 catagcgggc aatatagacg cagagcgagg acaagccgag gatccagacg gccggattct
    12601 tgacgacagc attccactgc atggcaccga cggatttttt cttttcttcg tcgatctgtt
    12661 cctgtgtctt ttccccatag tagacagccc cattcggcag gccctgcgtt tccgggcggt
    12721 cactcatgcc aaagtagatg aagaccgtac cgaccaggga aatgatgccg gccgaaatga
    12781 atcccataga ccagttatac atggagacga tggcagcaat ggcaaagtaa gcgaaagaag
    12841 agccgagatt gtggctcgtg aaccagatgc catataaggt cccgcgttgt ttattgctga
    12901 accactggtt aagggaaacg atagacggcc cggcaccata cgactggaac cagccattaa
    12961 agccccacaa gacggccagg gggacaaagg aattgaccag ccccatgccg accatggtaa
    13021 gcgaggaaat gaacaggccg aatgagaaaa accgtttatt gttcatccgg tctgccagga
    13081 agctgttggt gaacttccca agaccgtagg tcacaaagaa aatagaaccg atgataccga
    13141 tttccgtcgg cgtgacgatc cccgaagcca ggagcggctt cttgatgacc gagtaactct
    13201 ggcggatgat gtagtaaaag gcatagccga tggtaatgga cgcaaagacg cgccagcgca
    13261 tggcattgaa tttcttctta atcgccgcgg catccgtcat ctgcggagca tccggagcgg
    13321 tcttgaaaaa attccaaatc aggttgaaca tagtgaaaaa ctccttccgt tgcaaaaatc
    13381 cgtaatcata attccgcaat ttacggtttc tattataagg aacggatttt gctgacggta
    13441 gccgaaaaat tctgattttt ctgtaggagt ttttctgaca ttacatgacg cgtttggctc
    13501 cgatgtagcg ggcagcccaa taactgctgt ccatatcgga gatgctgacg ccatcgctgg
    13561 cggcattgat gaactggtga ttgccgatat agatgccgac gtgggaaatg cccgagtcgt
    13621 acgtcgagaa gaagaccagg tcgccaggct gcaggttagc catggaaacg gaataaccta
    13681 cgccatactg ttcgtcggca ctgcgcggca agtcgatgcc ggctgccgaa tagacgtagc
    13741 gcgtgaagcc ggagcagtcg aagccggacg gcgtcgaacc gccaaagata tagggaacgc
    13801 cgatatactg gttggcaatg tcgaggatat gcgtagccgc cggactttcg acgcgggcca
    13861 ggccattata tccgccattg ccgaaacggc ggataggacc gttgggcagg ccctgcccgg
    13921 taagggcata aaaagtcgac tggccgacga tgccgtccgt ttccagccca tggtcggcct
    13981 ggaactggcg gatggcctgt tccgtcccga cgccaaaaac gccatcgaca tcgacggtat
    14041 aaccattatt ggccagtgcc tgctggatca cgcggacttc attgtctgcc gtaacagccg
    14101 gcaaagcgtc cgaagccgcc cctccctgcg aagtcctgtt ttccggcatg gcttccccca
    14161 tgagggcttc atacgtagcg gcgccgatga tgccatcaca atccagtccg tggtctgcct
    14221 ggaactgacg gactgcggcc tgggtcgaat cgccatagtc gccatccgca tccgtatcat
    14281 aaccatgcgc cgtcaaagcc tgctggattt cagtaatctg atgtcccttg tcaccgggat
    14341 ggaacgtcgc accagccacc gacagggacg tcatgaagca cacccccagc ataacggcta
    14401 tcttctttgt cgtatccatg gaatacccct ttcccaaatt tgtaacatag ataattaatt
    14461 ataccatttt tttcgccaca tttatagtat ggggccattg tcgcacgaag gcttatacca
    14521 tcatgcgaga ttcctttcac tttatagctt gtagtacgga gtttgtagtt tttagcgaaa
    14581 acctttaaat ttaaagccat ctgctgacgg caaaaaagga actgtcgcac gaagattttt
    14641 gccatcatgc aaggcccctt ttactttata gtttgtagta cggagtttgt agtttgtaga
    14701 agattacttt aaatctttta ccatttgctg caaactaaaa actacaaact taaagctcaa
    14761 aaagaggctg tcttaatgcg acagcctctt ttttttacat acggatttta ttagaatttg
    14821 ccagctttgg cagcgccttc tacggctaca gctacggcta cggacgcgcc aaccatgggg
    14881 ttgttgccca tgccgaggaa gcccatcatt tctacgtgag ccggtacgga cgaagaacca
    14941 gcgaactgtg cgtcgctgtg catacggccc atggtatcgg tcatgccgta agaagccggg
    15001 ccggcagcca tgttgtccgg atggagtgta cggcctgtgc cgccgccgga tgcaacggag
    15061 aagtatttct tgcccatttc gatacattct ttcttatatg taccggcaac ggggtgctgg
    15121 aagcgggtcg ggttggtcga gttgcccgta atggaaacgt cgacgccttc gtggtgcatg
    15181 atagcgacgc cttcgcggac atcgtcggca ccgaagcagc ggacattggc ctgttcgctc
    15241 ttggaatacg ggatttcacg gacgatgttc agttcacccg ttttgtaatc gaactgggtc
    15301 tgaacatagg tgaagccgtt gatacgggaa atgatctgag cagcgtcttt gccgaggccg
    15361 ttgaggatga cgcggagagg cactttacga gccttgttgg cattgcggac gatgccgata
    15421 gcaccttcag cagctgcaaa ggattcgtgg cctgcgagga agcagaagca tttcgtttca
    15481 tcctgcaaga gcatcgctgc caggttgccg tggccgcggc cgactttgcg gtcttcagca
    15541 accgaaccgg ggatgcagaa ggactggagg ccgatgccga tagctttcgc agcttctaca
    15601 gccgtgcgtg cattggtctt gatggcgata gcggcgccaa cggtgtatgc ccagccggca
    15661 ttttcaaatg cgataggctg tacgtctttg acgattttat agggatccaa gccggcagct
    15721 gcacagatat cgcgacattc ttcaacgctg ccgataccgt attctttcag tacgccgagg
    15781 atcttatcaa ttcttctgtc gtagctttca aataattcca tgtttttgcc ctcctattgc
    15841 tgtctcggat cgatatacga agctgcttct tcaaagcggc cataatggcc ttttgcgtct
    15901 tccatggctt ttgccggttc aacgcctttc ttgatggctt ccatcatttt gccgaggtgg
    15961 atgaattcat agccgatgac ctggttgtct tcatcgagtg ccatgcgggt gacatagcct
    16021 tcggtcattt ccagataacg cgtgcctttg gctttcgtac cgaacatggt gccggtctgg
    16081 ctgcgcaggc ctttgccgag gtcgtccagg ctggcgccga tggggagacc gccttcggag
    16141 aatgccgtct gggtacggcc atagacgatc tggagaaaga gctggcgcat agcgacgttg
    16201 atagcatcgc agaccaggtc ggtgttgagg gcttcaagaa gcgttttgcc cggaaggatt
    16261 tccgaagcca tagcagcaga atgagtcatg ccggaacagc cgatagtttc aacgagggct
    16321 tcttcgatga tgccgtcttt gacgttgagg gtcagcttgc aggcgccctg ctgcggagca
    16381 caccagccta caccgtgggt cagaccgctt acatctttga tttcctttac ctgtacccat
    16441 tcgccttctt ccggaatcgg tgccggtccg tgatatgcgc ctttcgtgac gggacacata
    16501 ttcttaactt ctgttgaata taacatgttc tcttcaccct ttcaaaatgt atgagaaata
    16561 aaaagtagca cgtataggac tcagtccttc aactggtcgt atatctgtgt gaataaagcg
    16621 cgatacatgc tgcctttgcg ccacaagaat gaaaatgcgt gcatgacgga aaaatccttg
    16681 acgacaatgc gccgcagcgt cccgtcagcc agttctttct gaaccgattt ttcatataga
    16741 aatgcaatac cgcagtcgcg gcaggccagc cctttgacgg ctgacaagct gcctacttca
    16801 tagcgggccg ggaaatgttc cagtgtgtac ccctgttctg ccagcttatg ttctaaaatc
    16861 cggcgggcgc cggcatggcg ttcgcggacg atgacccgat agcgcatgag ttcagacagt
    16921 gtcatttcct ccggcgcttc atagcctgtg ccgcagacag cgacaatcgg ttcattagaa
    16981 aataagagcg attcatagca cccctggtca aaggagcttt cgacgatggc gaaatcaatg
    17041 gcaccatcat ccagggactg gagcagtttt tcagcatcgg caatttccat gcggagattc
    17101 atatcgtcgt tatgcttgag caggtcagcc aacttatcga gaatccccac gctagccgtt
    17161 ggcgtcgcac caaaagacaa ggagcggtta tgctgcaaat cacggagatg ctctttcaag
    17221 atggcatcgt catggctgat ggcagtcacg gcattccaaa ggatgtgacc agcctgggtc
    17281 agctgcaggt gtttcccctt gtaactaaag agtttggtcc cgtattcgcg ttctaaatag
    17341 cgaatatgct gagaaacggc aggctgtgtg atgtgcagtt ccgcagcagc ccgggtatag
    17401 ttcatgtgac ggcaaacgca caaaaatgta tggatacgaa aatctaacat agcacccttt
    17461 cctttctttg cgttttccat aagataattt tatgatattt caaaattttt atcaagaaaa
    17521 aagtgggcat tttatgtata cacatgggac gacttcgctc ccatcgattt cgatgccggc
    17581 ccgtttccgt ccgcttgtag tagacgaggc cctcatggta taataaggat aaacgattaa
    17641 cttatcaaat acaagaaagg atgaatcatt atgatgaaaa aaatgacgac actctgcgcg
    17701 tccctgctcc tggccctggg ctgcctgaca gccaatgcca tggattccca gaccctagtc
    17761 aataatccca gccgttaccg ggtcatcagc accagcccgg acggcatcgt ctatgccgat
    17821 atggattccc tccgggccat gcagaccatg gactatccaa acagcatcga aaacatgaac
    17881 ctgacgctgt acgtcgaaaa atacgccggc atccgcgacg acctggtctt ccagatgggc
    17941 caggaaatcc accagatcaa cgaatacaaa gcggccctgc acgccaacaa gcgcaaaggg
    18001 acatacgacc tgaaagccga tctgaccaag gtctaccggc ccgatggaac agactatgcc
    18061 gtcaagatcg acacggtcca gttcaaaaac gtccgcgaca tgtacacggc cctgcatcac
    18121 tttgccgccc tcacgccgca gaaggattag tggctcgtgg ctagtggctc gcagtggctc
    18181 tccttatagg agagctgtca gcgaagctga ctgagaggtt tctctatcaa catcgtttac
    18241 agaaaagggg ctgtcgcatg tgcgacagcc cctttttgag ttttaagttt gcagcaaatg
    18301 gtaaaaaatt taaaaccatc ccctggccac tagctgctaa ccaccagcca cttaaaatat
    18361 ttccctccgg cccgcagcta ttgtataata gaggcaatga atctttatta tatgaaatga
    18421 aggggacctg acatgacgaa tgaaaaatat atgaatatga tccgcaacaa tccctatgta
    18481 atcgaccgca tcgaaaatcc gtcggaagaa atgcagctcc tggctgtcca gatgaacggc
    18541 ctggccctgc aatacatcaa gcatccttcc gaagccgtcc agaaagccgc cgtcgccaac
    18601 acggcgcggg ccatccaatt catcgaccat ccctcggaag acatgctcct gcaagccgtc
    18661 cagtccggct ggaacaactt gagctatatc aaaaatccgt caccggaact catcgacgcc
    18721 gccctggaac agtccggctg ggccatcaaa tacgtcaaag acccggacga agacctgcag
    18781 ctgcgggccg tagaaaagaa ttacgacgcc atcaaataca tccagcatcc gacaccggcc
    18841 gtccagctgg ctgccatccg cggcaattac gaagccctgc gctacatcga aaaaccgaca
    18901 gaagaagcga aagaactggc catcagcgaa aatgaacagg ccatccgcct catcccggac
    18961 atcagcaaag aagagttcct ccacttcctc agccgcaacg tcctggtcag caaatatatg
    19021 cccaagggca tttccctgac gcccgatgac tggcgcaaca ccttcaaggc cgtcttgtcc
    19081 cgggatgacg tgccggaaga ctacgtccgc aacttcatta cctgcagcgc cgtcgacctg
    19141 acgccggctg ataaagtcct cctcgtcgac gattgcggca gcaagaaggc ccgccgcatc
    19201 acagtagatg aaaaattgac attgaaatag gagcgaaatc atgaacttta cagatacttt
    19261 caaaagcgta gaattagtcc tcaaagccgg cgaagtccca ctgctcgtcg gcgaaacggg
    19321 tatcggcaag acctccctgg cccgggaact ggctgccaag aacgactgga gcctcgtcgg
    19381 catcgacggc aacctcctca aagaagggga aatcggcggc ctgcctacgg tcgaagcggg
    19441 cacgacgatt tacgccgttc accataaact ccgcaaaatc gacgaagaaa tcgccaaggg
    19501 caagacggtc ctcttgttca tcgacgaaat caaccgctgc gaccacgccg tccagcagga
    19561 actgatgaac ctcatcctca accgtgaaat caacggttac gtcttatcac cgtcagtcca
    19621 tatcatcgcc gccatgaacc cggccgattc ctacgactac gaagtcgtcg aaatggatgc
    19681 ggcccagcag aaccgcttcg tctggctgta tatggaagcc gattacctgc agtggctcga
    19741 ttgggcctcg acagcgggca tcgaagaaaa agtcatggaa ttcatttcca ccttcccgga
    19801 atacctggac aagaccaatg aagacgatat caatgcgacg ccgcgcagtt acgaacgcat
    19861 ttccaagctg tactacattt ataaacagga cccgtcggcc atcccgcagg gcgtcttcta
    19921 caacgtcatc cgcggcaacg tcggcaaact catcgcccag gaattcgtcc atttcgtcac
    19981 gtccgacgcc cagcccctca tcgcttatga cgacgtcttc ggcggaccgt ccctggcccc
    20041 ggccctgtcc agccggattg ccagcgaaag ccacacccgc ctctatctgg ctgccaagaa
    20101 tatccttaaa cagctggaac atgaactccg cctcgacgac agccaggccc aggcgctcct
    20161 gtcccgcttc gtagccttcc tgaccctcta cccggtcgac ctgcgcctcg gcgtcatgaa
    20221 ggacatgaaa tccgccaacc ccaaagtcta tgcctgcgct ttggataacg acgacttcat
    20281 cgacagctac ttcgccgcct tccgctccct gcgataacag cttatggaac agtcactgcc
    20341 cattgcggcc aatctactct atcagcaggc atctatcgta gccgacgccg tcctcgccga
    20401 acagcgccgg acagggagcg tcccggacgt gccggccgat ttccaaaaga aattctacgc
    20461 cttcctggac cgcatcaccg gtcacttgat ggaagacaaa gacaatttct tcggctattt
    20521 cctcttccaa atggtcaagg acatccgctt cgacatggct tcaccgacgg ggaccaactt
    20581 caaggggacg cggtatcacc tctatttcaa ccccatgctc ttcctgcccc tgtcgccgga
    20641 acagatggag agcaccatca agcacgaaat cctccacgtc gtatcgctcc acctcatccg
    20701 ggccaaagaa ctgcggcagc aatacagcaa gctggccgtc aatctggcca tggacgtcgt
    20761 cgtcaacacc tacctcgacc acctgccgcc cttctcgacg accctggaat gggtgaacat
    20821 gaactatgcc ctgctattaa agccctttga aagcctggaa tactacgtcg ataagattca
    20881 aggcgccctg gacctgcgga ccgacaagaa ggacctgccc gaatcggaca gcgattccga
    20941 cgaatccatc gccgtgtcct acgacccggc caagacccat gatttatggg acgaaggcga
    21001 cgacatcgac gaagagacgc tgcgcaaatt cacagaaaaa tacatcgacg cgtcatgcaa
    21061 aggcgaactg tcgaactatc tggaaagcat gattgcagcc ctgaaagatg cccaggaaga
    21121 tctgccctgg cactggtacc tgaagaagct cgtcggctcc gtcaccagca cctggaaaaa
    21181 gacgaccatg cgccgcaacc gccgccagcc agaacggctg gacctgccgg gctgcctgcg
    21241 ctcccatacg gccaaaatcc tcatcggcct ggacatcagc ggcagcatca ccgacgccga
    21301 attccgccag gccatcggcg aagtcctgca cctggtccgc tgctacaacc atgaaatcat
    21361 cgtcgccgaa tgtgacgatg aaatccgtcg cacctaccgc atccgcacta tagacgacgt
    21421 ccgcggccgc ctggacatcc gcggcggcac agcctattcc ccggtcttcg cctatgccaa
    21481 tacacagcgc gtcgacctcg tcgtctactt caccgacggc aaaggcgaag aaaaactcca
    21541 gacgccgccc aaaggctaca aagtcctctg ggtcctctcg ggcaaaggag ataaactctc
    21601 cctgaaaaag cccttcggcc tggtcaaacg cttgaccaaa cttccagaat acgacccgtc
    21661 cctcgacttc gacgacgtag aaaaaggcgg cttctccatg aaccaccagg aagggatcag
    21721 tatgccgtga gtaaaaaggc gctgtcgcac gaagacagcg cctttttgag tggctagttg
    21781 ttagtagctc gtggctcata attcgtaaaa cctgccatta tactcgaatg ggctgtggcg
    21841 aaatctccct agcgggctcg ccacgtgcga gcccctacgt gcggccgctg gccgcttttt
    21901 gcgacctgca accggccgcc tgcggcctgc gtttattttc tcatcaccga taaaagagag
    21961 cattttattt tcgtttactt cgtttcctgt tttagcgaat caaaaatgta acgccaatgc
    22021 aacgaaaaaa ctttcgttgc attggcgact tcattcaatg attattctcc aggaacgaaa
    22081 gaaacgggaa cattgacgat tatcgtcatt tatgttatgc tacatataga aaaaggtgtt
    22141 gcccggaaac ggtcagcccc attgtttaac gaagtaatcg cctagagttg tggagctgca
    22201 aggcgattat ttttttatta caaagttatc catgggcttc accccatttc tggggcttga
    22261 ttaaccgcct gccgtttggc aacaccttga ttcagtatag ctaaaaatat aaaatttgtc
    22321 aaaaaagagc ttgtcgcaca attttacgtg cggcaagccc ttttggcgtt tctgatggat
    22381 tcagttaaaa taatttttcg tagagataac ggatatcgtc cagggacaat tccttgcagt
    22441 tgttgactaa gagacgggtc tgctggcttc cggcttttac caggaaatcg aggtcttctt
    22501 ttttgacgcc gaattcgtcg agtttcgtcg ggatatggac tttggcgacg acatctttga
    22561 tttcagaaat gatataatcg gctttgctgt ctgctgtttc tgcatatttt tcaggataga
    22621 cggcatcgca gagagcggcc agccgttctg tgcaggcatc tttgttgaat tccatgacat
    22681 gcgcaatcag gatagcattg gaaacaccat gggcaatgtg gtatttgccg cctaacggat
    22741 aggagagggc atggacagcg gtcgtaccgg aaccggtaat ggcaacacca ccgtaaaaag
    22801 cgccgatgag catttccgtc ttggcatcca tattatcgcc atccgcatag gcttcacgga
    22861 tgttatggaa aatcttcttg gccccttcaa tcgcataggt atcgctgaga atcgtcgcct
    22921 tgtttgacgt aaaacattcg acgacgtgag ccagggcatc gacgccagtg gcagccagga
    22981 ctttcggcgg caaatggcgg atcatttcgg cgtcgaggat gacatagtcc ggaatcatgc
    23041 aattattgac gatgccttgt ttagactgtt cttccgggat ggcgacaatg gaattgcagg
    23101 ttgcttccga accggtcccg caagtcgtcg ggatcatcag ggatttgata tatttcttgg
    23161 caatcgtcgg atctttcagc aagtcgcgga cgccatagtc ggcaccgatg acgatgctgg
    23221 ccagcttagc cgcatccatg acgctgccgc cgcctacacc aatgaccaag tcgatattgg
    23281 caacactgac ttcatcgatg acgcgttcca catcctggta ggaaggttcc gaagccaaat
    23341 catcgataac cgtcaatttt acagccgagc aaatttcttc gacccgtttc gtaaggcccg
    23401 ctcctcggac tgccttatcg gtaaacagca agacggactg tgccttttcc cgctgcaata
    23461 cttcattgat tttttctaca ctaccagggc cggcataaac cacgcccgga atggtcaact
    23521 gacattgttt catagtataa aagcctcctt aatattgaat catgcagtct tttcttccac
    23581 tttcattggt tcaattttac ctaaataaaa aatgtaattt aatactgcac ctaataacaa
    23641 taatgcagtg aagagcatcg caaattcaaa atgccccgta gccccgacaa taaaaccagt
    23701 aacaatcggt ccaactacgc caccaaaatt agcaacacaa ttttgaatcc cagcaacaat
    23761 gcctgtcata ttacgtggtg caatctcact agggatagcc catgtctgag aagcagcaat
    23821 aacagtaccg gatttagcac aacacaataa gaacagtgtt acataaatag attcagttac
    23881 ggccgcaaac ccaatggtag aagctaaaat caagccacca cacagaaaca tttttcgtgt
    23941 ttttgttaat gagaatttgc cagatgaaac gattttatcc gaagaccagc ctgcaataac
    24001 ttcaacaata gcaccacaaa gtaatggcaa tgaagcggca aaacccattt gagcaaagct
    24061 aagacctcta tctttcatta aatacatcgg taaccaagtg ataaaaaaat aggaactata
    24121 gttagttgca aaaaagccaa tacacattgc ttgcatattt ctaaacttta acaattcata
    24181 ccatttacaa ggcatcgcct tattgcttgt ttcatcagta tcctgccccg acttaatata
    24241 tgccagttcg gcagcattga catgcttatt tttcgctgaa tcatctttaa aaaatgcaaa
    24301 ccaaactata atgggcccct tgtcaagacc acgttctaaa attttaagag tggcagtaag
    24361 c
//
LOCUS       DAWCYS010000006        22199 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_136_length_22199_cov_10.386425, whole genome shotgun sequence.
ACCESSION   DAWCYS010000006 DAWCYS010000000
VERSION     DAWCYS010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 22199)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22199)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22199
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136_length_22199_cov_10.386425"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4838 a         6443 c         6225 g         4693 t
ORIGIN      
        1 ttgttagtgg ctcgtggctt gtttttgcgg ccttatggcc gcatcgtagg ggccgtccca
       61 aagggcggcc cgcctgaaat gtgatgtaca tgccatacat aacgggctcg gactcagatg
      121 ccgctgcgcg gcgacaaact tcaaactgac aacatcgctt cgagtaaccc tcagctcgta
      181 aaaacgcccc gaccggggcc tgcgtagggg ccgatcttta acctctcaga ccgcctttgg
      241 cggtcagctc ccctataaag ggagtcttgg gcggcccgct gtgatatttg accacagcct
      301 attcagaatg atggtgagct tttacgagcc acgaaccacg aacaacgagc cacagctttt
      361 ttcatggtga tatacataac aggtccatga ggcatggttt tgcgaactgc gaccggggaa
      421 tagtacattt tttcacataa cggattgact tttcttattg tgaaatatat aatggataca
      481 aagaataaaa gcaaggacaa agatgtctta cgagtacaca ttctgccctt gctctttttt
      541 tactctttgt caaatttaaa cgaaaggatg gatggttatg ctttcacgga ccttgttggg
      601 aatgagatgg ggcgacttat cggaagatac gcgccagatg ttattggacc gcgccgatat
      661 cgacggtttt aatggcgctg atggagaccg ttatagtcga gggacgctcc atttccgcaa
      721 taccctcatg ggaatccagg cctcagtgga aacagaccgt ttttccggtg atatgggaaa
      781 caacggcatt ttcattgacg atgatgctgt catttcccgc atctaacaat catttgaata
      841 agaacacctt gcataagccg ataatgataa aggctgcaat gatgatgaca ctaataaccc
      901 gagtcttagt aaagattttc gggttatttt gtatattttt gggcgggact ggcggtggta
      961 cttcctgttt agtgccgcaa atcgggcaca tgaggacgcc gtcgcgcagt tccgtaccac
     1021 atttgggaca aatcataagc tcaactcctg ttcttctttt atgtatacta tagaaggctg
     1081 tacccgctgt gtcaagaagt tatctttttg tgaggaaaat atacatataa aaccttgtgt
     1141 atttggcgaa aatagcatac aattttattt atctgctgtt ttttattacc agagaaaagg
     1201 agatacagcc ttgaagaaaa aaattggctt gagttcccaa attttaatcg gcctggccct
     1261 cggtgccctg gtcggttatt tgttccccga tttcggcgat aagctgaaac ccttcggtga
     1321 tgccttcctg cgcatgatca agatgattgt cgtgcccctt attttcagta ccctggtcat
     1381 gggtattgcc ggtacgggcg attttaaaaa gctcggccgc ttaggcggca aggccatcat
     1441 ttggttcgag ttcgctacga ccatcgccct ggccatcggc ctgttgttca tgaatattgc
     1501 agaacccggt gtgggcgtca ctttatcggc ttcggcagcg aatgcttcca atgccgcagc
     1561 tgccagccag cacagtgtcg acctcgtcga ctatgccgtc cacatcatcc cgaccaacat
     1621 cgtcgatgcc atggcccggc aggatatgct gcagatcatc ttttttgcct gcttctttgg
     1681 cgtcgctgtc gcccatatcg gcggcaaagg tgaaaccatt gtcgatatct gccagagcgt
     1741 ggctgaagcc atgttcaaag taacgggcta tgtcatgcac tttgcgccta tcggtgtctt
     1801 tgccatgatt gcctataccg tcggcagcta tggcattgcc atgctcatcc ccttgggcaa
     1861 gctcatcttg tccgtcgccg gtgctacggc gctgttcatc tgtgtcgtcg ccctcattgc
     1921 cagccttttt acgggcatca atttcttcca cgtcatccgg gccttgaagg acatcctctt
     1981 cctggctttc tcgacggcca gcagtgaagc cgccctgccc ggcgccatga agcgcctgga
     2041 agaattgggc gttcccaaga cgattgtcac ctttgtcatg ccgacgggtt attccttcaa
     2101 cctcgacggc tcgacgctgt acagttcagc cggtatcttg ttcattgccc agctctacgg
     2161 tatccagatg cccattgagc agcagctact ggtcatggtc acgctcatgc tgtcgaccaa
     2221 gggcattgcc ggtgttcccg gtgcgggcat catcgtcgtc gctgcgacgg cagcggcctt
     2281 caatctgcct acagaaggcg tcgccatcct cctgggcatc gaccgcctcc tcgacatgat
     2341 ccgcacgatc tgcaacgtct gtggcaactg catggctacg gtcgtcgtcg cctgctggga
     2401 aaaggaattg aacaaggcca cgttcaatga aaagtaccgc gcttttttgg gcggaaaaaa
     2461 taggtgagta ttcgccaaag tagactaaat ctgttataat aatagggtaa ataagtgctc
     2521 cgtaaggatt gcctgacgga aaacttcatc tatgaagcaa ccgtgctcct ctgcaaagac
     2581 ttgactgcgc agatgaggcg aaagcacaat tatccgaagg gactgtcgca cggaggcttc
     2641 tgccatcatg cgtggtccct tttgattata gtttgtagat gagggctttc aattcaaaga
     2701 ccttcactaa aaactacaaa ctggaaactt aaaactcaaa aaggggcgtc gcacgtgcga
     2761 cagccccttt tttctttagg aggtacgaat atggaaacga gagtcgcttt aatcggtatt
     2821 atcgtaggga atcgccagga atcgactaaa ttaaatgaga tcctgcatga atatggccag
     2881 tatgtcgtcg gccgcatggg ggtcccttat aaggataagg atatcaacgt catcagcctc
     2941 atcgtcgatg cgccgcaaga cgtcatcagc gctttgtcgg ggaaattagg gatgctcccg
     3001 gaagtcagca ccaagacgat ttacccgcct atgccgcagt aggaggcgcg tatgctgaaa
     3061 attgcgattt acggcaaagg cggcatcggg aaatcgacga tgaccagtaa tctggccgct
     3121 gccttcgcct atttaggcaa gaaagtcatc cagatcggct gtgaccctaa ggccgattcg
     3181 acgttcaatc tgctcggcgg ccatcccgtc cagccggtca tgaattatct gcgggaacac
     3241 gacgaagatc cagaatccat cgaggatatt tcccgcatcg gctatggcgg tgtcctctgt
     3301 atcgaaacgg gcggtcctac gccgggcctg ggctgtgccg gccggggcat tatcgccact
     3361 ttttctttgt tggaagattt aggcctcttt gaaaaatatg agcccgacgt cgtcctgtac
     3421 gatgtcctgg gcgacgtcgt ctgcggtggc tttgcagcgc ctatccgcga aggctatgcg
     3481 gaacaggtcc tcatcgtcac atcgggcgaa aaaatggctc tctacgcagc caacaacatc
     3541 aatacggctg tggccaattt tgccgaccgc agctatgcca aggtccgggg tatcctcctc
     3601 aaccgccgca acgtccccga cgaagaagcg aaggtccggg cctttgccga aaaggctcac
     3661 ctttccatcg tcggcgacat tccccgcagc gacgacatca accgctatga agatctgggc
     3721 cagaccgtca tcgaaggcga tccggacctg cctatcagcc atatcttcct cgatttggcc
     3781 caaaaattat tacaagacaa ggaatgatta tgacagaacg aaaaccttat gctatcaccg
     3841 tgcaggacct ggtgacgcgc ggccgcgatg atatcccggc tgaactcgtc acgtcgacgc
     3901 acctcatcta taattctccg gctgccctgg ccttcaactc gcctggggcc cagggcttcg
     3961 gcgtcaaacg ggcaggcctg tccattcccg gatcggtcat gctcctggtc ggaccgggct
     4021 gctgtggccg caatacgacg atcctcagcg aaatgggcgg gtacagcgac cgtttcttct
     4081 tctatatcat ggatgaaacg gacatcgtca cgggccgcca tctgaagaaa atcccccagg
     4141 ccgtctgtga agtcgtcgac agcctggctg aaaagccgtc agtcgtcatg atctgcatga
     4201 cctgtgtcga cgcccttctg gggaccgata tggaacgggt ctgccgcaaa gccgaaaagg
     4261 aagcgggcct gcccgtcgtg ccctgctaca tgtacgctct gacccgggaa ggccgcaagc
     4321 cgccgatggt cgacgtccgc cgggccattt attcgctgct ggaaaaacag ccgcgccgcc
     4381 gccggacggt caatctcctg ggctatttcg cgcccttgca ggacgactgc gagttgtacg
     4441 acatcctgag aggcgtcggc tttaatcaga tcaatgagat ttcccgctgt cccgactttg
     4501 ccgcctataa ggccatgtcc caggccaacg tcaacatcat cctcaatccg gaagcgcgcc
     4561 tggctgccca ggatatggaa aagcgcctgg gtattcccag catcgagctg acccgtctct
     4621 accaggtcgc ccagatccac aaacagtatc aggccctggc tcaggtactg gggacgacca
     4681 tcgatgacag tgcctggcat gaacgggccc aggccgtcgt tcgggctgtg cgggaaaaat
     4741 gcagcggcac gaccgtggcc atcggcgaga tgcagaacgg caacgccttt gaaatggccc
     4801 tggccctgac ccgctacggt ttcgtcatcc gcgaaatcta cgccaacctg gggccggacg
     4861 atttcgtcta cctgcgccac ctggcccagc tgagcccgga tacgcaggta tactccaatt
     4921 tgtcgccgtc catgctctat taccggggac aggaccatcc tgtcgacgtc accatcggca
     4981 aagatgccgc atattatcat gaacaggccg cccatctctc gtgggatgaa gatatccagc
     5041 ccttcggtta tgccggagtc atttcactgt tacagcggct ggagcaggcc ttgttggaaa
     5101 ggaaacatcc atgaaaggtt tatggaaata tatttcaccc tttgcaccgg acgactccgg
     5161 cgcggcggcc gtcctctacg gactgaacgg catcgtcgtc atttgcgatg ccggcggctg
     5221 tgccggcaac atctgcggtt tcgatgaacc gcgctggttc agtgggaaaa agagtgccgt
     5281 cttcagtgcc ggcctgcgcg atatggacgc catcctcggc cgcgacgacc gcctggtcga
     5341 taaactctgc caggctgccg aaacgattga tgccgacttt gccgctatcg tcgggacgcc
     5401 ggtcccgtcg gtcatcggca cggactataa ggccttgaag cacatggctg aaaagaagct
     5461 gtccatccca gtcgtcacag tcgatacgac gggcatggat tactacgaca agggcatcga
     5521 aaaggcctac gacgccttat tcccggtcct ggccgacccg tcgcagcagg ccgaagccga
     5581 tacagtcggc gtcctcggcg ctgttcccct ggaactgatg caccctggcg atatcgaatg
     5641 gatcagccag tccctggtcg aagacggctg gcagcagatt ctcttgttcg atgaaatcga
     5701 cgattaccgc aaggccggca aggcctccct caatctggtc ctctcaccgg ccggcctgaa
     5761 ggccgctcaa tatctgcaga agaccttcgg gacaccttat gagctccatt acttcggcct
     5821 cgatgccgtc gtcgattttg acgacaacct cttttctggc aagaacgtcc tcatcatcca
     5881 tcaggctgtc gctgcctcgg ctatggccgc catggccgaa gaagccggtg ccgcttacgt
     5941 gacgacggct tcgtggttca tggggctccc tgagccgcag gggacggact gtgtccggct
     6001 ccgtgaagaa gacgacctgc gccgcctggc cgacaacgac cagttcgaca tcatcctcgg
     6061 cgatgcctat ttccgccggg ccctgccgaa cttccacggc caatacgtcg atttcccgca
     6121 tttcgccgtg tccgggaggg gataagatgc atacggaacg gatttgtgtc tttggcatcg
     6181 tccagggcgt cggctttcgg ccctttgtca gccgcatcgc agcggctcat gccattcacg
     6241 ggacggtctg caataagggc tcttatgtgg aaatcctggc ccaaggttcc ccggcggcgc
     6301 tgatggcctt ccacgatgat ttggaaggca aggcgcctga acggtcggct atcctgaaag
     6361 tcgtcacgac gacggaagac cggccgccgt acgaggattt ttccatcgtc gagagcgcca
     6421 gggaaaaagg cgatatcttc gtgtcgccgg acattgccat ctgcgatgcc tgcaaagcgg
     6481 agctcttcga taagaccaac cgccgctatc tccacccctt catcaactgc acggcctgtg
     6541 gcccgcggct gaccatcctc gatgccatgc cttacgaccg cgaacggacg agtatgggcg
     6601 agttccccat gtgtccccag tgtgagtatg aatataccca cgccgaaacg cggcgttacg
     6661 atgcccagcc ggtctgctgc aacgactgcg gtccggaagt ctatctcctg gaccggcccg
     6721 aacggcgccg tcaggccatc attgcggcca gacaggcgat tattgatgga aaaatcatcg
     6781 ccgtcaaggg catcggcggc ttccacctct gctgtgatgc ccgcaatgaa caggctgtcc
     6841 agaccctgcg tcagcgcaag cggcggcctg taaagccttt tgccgtcatg atgaaggacc
     6901 tcgacgccgt gcgccggcag tgcatcattc ccgatggggc ggaaaacgtc ctcgacggcc
     6961 atcagaagcc gattatctta ctgcccaaga aaggcagtca gaccctggcg ccgtccattg
     7021 caccgggcaa tccgtctgtc ggcgtcatgc tgccctatac gccgctccat ctgctgctct
     7081 ttacttatga cgacggcctc gccgtaccgg atgcactggt catgaccagc gccaatgagt
     7141 ccggcgcgcc tatctgtcac gatgacgacg aagcgcggca ggaactgtcc ggcctctgcg
     7201 acctcatcct gagccacaac cgccgcatcc gcctgcgggc cgatgattcg gtcatggact
     7261 ggtatgacgg caagccctat atgatacgcc gctcccgggg ctatgcgccg ctgcctttct
     7321 tctatggaaa gggaagcggc aaggatgtcc tggctatggg tggagaattg aagaactgtt
     7381 tctgcatcgg ccgcaatcac ctgttctatc cttcgccgta catcggcgac atggccgata
     7441 tacggactgt cgaagccctg cgccagtcca tccggcgcat gacgacgctc ctcgaagcca
     7501 agccgtctgt cgtcgcctgc gacctgcacc cgcgctataa cacgacggcc gtagcccatg
     7561 aactgggcct gcccgtcgtc gccgtccagc atcactatgc ccatatcctg tcctgcatgg
     7621 ctgaaaacga ctgggaagaa ccggtcatcg gcatcgcctt tgacggtacc ggctacggga
     7681 cagacgggac gatttggggc ggcgaagtcc tggtcagtga cgtccatggc tttcagcggt
     7741 ctgcgtcaat ccagcctttc tggcagcgtg gcggcgacgc atcggcccgc gagggctggc
     7801 gcattgccgt cagcctcatc gacagcttgc agaaggaccg gagccaggcg gcgtccatga
     7861 tcgaagccct gcacctttgc agtgaagccg aacggcaggc ccagttcttc ctggctgacc
     7921 agcagatcaa cagcgtcttg tcgaccagtg ccggccgcct cttcgacggc atcagcgcta
     7981 tcttggggat ttgccgggct tcgacctttg aaggccaggc gtcgatggcc ctccagtttg
     8041 ccgctgaggc ctgggaaaaa gaccgtcccg caccggatgt gacgcccatg gcgcccatcc
     8101 atggccaaga gcggctgctc ctgccgacgg gtgtcctggt ccggcagata ctggaaggcc
     8161 gcctggccgg agaagcggcc ggctgcctgg cctatgcatt tcatcggggc ctggccgcca
     8221 tgattgccgg ggcctgcctg gccgtgcagg aagaaacggg actgcacgcc gtggccctga
     8281 gcggcggcgt cttccagaac cgcctgctcc tgcgcctttg cgaccagacc ctgcagcacc
     8341 tgggcttcac cgtcctcaag cacagcacgg tcccgcccaa cgacggcggc atcgccttag
     8401 gacaggccgt ttacgccgcg tatgcgtagg ggccgcctgg aaggcggccc gcaggtcgca
     8461 ggtcgcaggt cgcaggtcgc aggtcgcaag gctcccctga taggggagct ggccgccgta
     8521 ggcggtctga gaggttgccc gtccggactg atggtgtaca cgtcgtgcat gtattcgtag
     8581 gcccgcaagg ctcccctgat aggggagctg gccgccgcag gcggtctgag aggttgcccg
     8641 ttgttcgatg aacgaaatta attgattata cataaaggag gaattatcat gtgtgtaggg
     8701 ttaccggcac gtgttatgaa aatacagaat ggaatggctc tcgtcgatgc ttccggtgct
     8761 aaacgggaag tatcggcagc tcttatcgac gaactcgatc ctggcgatta cgtcatggtc
     8821 cacgccggca tggctatcgc caagattacc gataatgatg ccgacgaaac agacgatatc
     8881 ctggaggacc tgaactgatg gcagaagcaa agaagcggac ggccctggat attatccgca
     8941 gttatgacgg gccgaaaatc cgcattatgg aagtatgcgg gacccatacg catgaaatct
     9001 tccgccaggg catccgccag atcctgccgc cccaggtcga cgtcatctcc gggccgggct
     9061 gcccggtctg cgtcacgcct gtcggttata tcgaccaggc catctggctg gccctggaaa
     9121 aaggcgttac gatctgcacc tttggcgacc tcgtgcgcgt accgggcagt acgatgagcc
     9181 tggccgatgc ccgcagtcag ggaggtcatg tggaaatcgt ctattcgccc ctcgacgcct
     9241 atgattatgc caaggcgcat ccggacgaag acgtcgtctt cctgtctgtc ggctttgaaa
     9301 cgacgacacc gccgtcgtgc ctggctgtca agcaggccgt cgccgacggg atgacgaatt
     9361 tttccctgct cacggctaac aagaccatgc ccatggccta tgaagccatg aaggacagcg
     9421 ccgacgcttt cctctatccc ggccacgtcg acgccgtcat ggggacccgc tctggcgaag
     9481 ccatgctgaa agaaggcgta tccggcgtcg tcgccggctt tacggccaaa gaactgctca
     9541 cggcctttgc cgtcatcctg gtcaaattcc ctcaggggaa gcccttcttc aagaactgct
     9601 atccccgtgt cgtcaaggct gaaggcagcc cggccgctca ggccctgata aaagaagtca
     9661 tggaaccgtg cgattcggaa tggcgcggct taggcgtcat cccccagtcg ggcctgcagc
     9721 tgcggcctgc ctttgcagtc tgtgacgccc gcaagaaata cggcgtgccg gctatggaag
     9781 ggcgcgccaa tccggcctgc cgttgcggcg atatcctgca ggggaaatgc acgccgcccc
     9841 agtgcccgct ctttggcaag gtctgcacgc cggaacatcc cgtaggggcc tgcatggttt
     9901 cccacgaagg ggcctgctcg gcttattatt tatatggagg caatttacat gagtgatgtc
     9961 attaccttag cccacggcgc cggcggcaaa cagactgccg aactcatcga ccgtgtcttt
    10021 aaagctcatt tttccaatcc ccagttcacc ggggacgacg ccgctgtcct gcccatcggc
    10081 gggactaacc tggcttttac gacagacggc ttcatcgtct cgccgccgga atttcctggc
    10141 ggcaatatcg gcaaactgag catctgcggg accgtcaacg acctgtcctg catgggtgcc
    10201 aagcccttat atctgtcgtg tgccttcgtc atcgaagaag gcttccccct ggctaaagtc
    10261 gaagaatatg cggcggctat ggccaagacg gcggctgaag ccggtgtgcg catcgtcgcc
    10321 ggcgatacga aagtagccgg gaaaggccag gtcgaccacc tgttcattac gacgacgggt
    10381 atcggccaga tcgtagacgg cgtccatacg tcggggaccc tggcccgtcc cggcgatgct
    10441 gtcatcgtca gcggcgacgt cggccgccat ggctgcacga tcctcctggc ccgcaaccag
    10501 ttcggcatcg atgccgatgt gaccagcgac tgcgcgcctt tatgggggac tgtcgaagcc
    10561 atgctggata cgaccaagga catccacgtc atccgtgatg cgacccgcgg cggtgtcggt
    10621 acggtcctct atgaaatcgc cggccagagc cacgtcggca tccacttgga cagccaggcc
    10681 atcccggtcg acccggccgt ccgcggcgtc tgcggcatgc tcggcctgga accgctttac
    10741 ctggcctgcg aaggccggct ggtcatcttt gcgcccaaag ccgtagccgg cgatattgtc
    10801 gccaccctgc gccagggcaa atattcgaaa gatgcggcca tcatcggcga agttacagcc
    10861 gaccagcccg gccgggtcat catgcgtacg gaaatcggcg cggaaacgct gctgccgccg
    10921 ccaggcggtg aactgctgcc gcgcatctgc taaggaggag ggagagcatg atttacgaaa
    10981 atgaaattca ttcaccccag gtgccgcggt atcacgtcac accagacggc gccgaagtcg
    11041 ccatcagcca ggcgtcgttt cccaagcctt tccacatcgg cctgaaatat gcttcgccag
    11101 cccggggcca ttggaccatt gcccattcgc ccatgctcat ccctggctgc cacgaagtct
    11161 acgtttgctg cgcctgctgc cttcacggcg tcgtcttgtc ggctgatgaa gtcccggacg
    11221 gggccggccg cttctctacc gtcaccttga cgaacgagaa cctgataaag ggcaacctgg
    11281 aagaaatgat gatcgacggc atttcccata tcatcgacga tatgcccgag cggccgaagt
    11341 gcatcgaagg ctttaccagc tgtatgcagc acttcctcaa tatcgacctg cgcgtcgtct
    11401 acgggacgct gcgccagaaa tatcccgaca tcgatttcat cgacggctat atgattccga
    11461 ccctgcagcg gcgctatacg ccggacatcc tcggccggcg ccagctcgtg cgggctgtcc
    11521 agcccctgcc gaaacagaag gccgtcaatt atgtcgtcaa ttactatccc gtcgacgaag
    11581 acaatgaatt gacggctatg ctccgccagg gaggctatgc tatccacgat ttcgctgcct
    11641 gccagactta tgaagaatat aaggccatgg gtgaatcggt ggccaacatt tacttcctgg
    11701 aaaatgccgg tccggcagct caggatatgg gcaagcgcct ggaccagcag gccctgtacc
    11761 tgccctatgc ctatgagtac ggccggatgc gccgcaacct gcaaaaggcg gcggatgccc
    11821 tgggcctgac catgcccgat tgcgacgcct atgaacaggc tatcgaagaa aaagtccaaa
    11881 ccctgcggga acaagtcggt gatacgcaca tcgccatcga ctatacggcg acgccgcggc
    11941 ccttggaact ggctgcgttc ctcttgaccc acggtttcaa cgtctacgcc atttatctcg
    12001 acgaaatgtc gcctgatgaa aaggaaacgt tccagttcct gcgggaacag tatccggacc
    12061 tgtccctgcg ttcgacgtcg catttcaagc gccgcctcct gccgcgtgac gacagtaaga
    12121 agtacgggaa agtcctggcc atcggccaga tggcagccta tttcacggat acgaagtatt
    12181 tcgtcaatct cgttgaaaac ggcggtttct atggctatac gggcctgttt aagattttag
    12241 atttgatcgg ccaggccaat gccgccgaaa atcccaagat gcgggatatc attcaaataa
    12301 aagcatgggg gtgtcatggt tgaaacaagc acaacgcgtc ttatcgacgt atgccgtcga
    12361 catgttcggc attgcatcgt cgctctttga attaggcggc ctcatcgtca tgcacgatgc
    12421 tgacggctgt aattcgacat acgatacgca cgatgaaccg cggtggtacg atacgccgag
    12481 catggtctac gtatcgggca tcaatgaaat cgataccatc cagggcaatg accagcgcct
    12541 catcgacaat atcgccgaag ccgtagaagc ggcccatccc cgctttgtcg ccatcggcgg
    12601 cagtcccatg ccgaatgcca tcggcacgga cttcaaggcc atcgagcgcc tggtagcggc
    12661 ccggacgggc ctgccgacgc tgggcttccg gaccgacggt atccacagtt acctgcccgg
    12721 tgccggcggc gcctatgtct ggctggccaa gacctttttg aaggcgcctc atcgcaagcc
    12781 gcagaaaccg gcgaaacggg tcaatctcct gggcctgacg ccgctggact tttccgtcgt
    12841 cggcaatgcg acgacgctca agcaaatcgt caccgatgcc ggctttaccc tgcagagttc
    12901 gtggtccatg ggcgataccc tggacacgtt ggccgaagcg gccaatgccg acgtcaacgc
    12961 tgtcctgtcg tcgacggcct tctacctggc ccagtacatg cgcgatacct acggcatccc
    13021 ttatgtcgtc ggcatcccca tgggtgaaaa ggggacggct gactggctgg aagccttgcg
    13081 gaactgcgat tcgtcgtatc tcacaggctt tacgggacag gaaaagtaca tcaaagccca
    13141 gtatgccctg gccgacctca acgcctggaa gacggagccg gtctacgccg atacgccctg
    13201 tgacgtcctg gtcatcggcg aaccggtcta cctcttgtcg atgaaggcta tgctccagca
    13261 ggaaatgggc atttccgacg tccgcatgct ctgccccctt gccgatgcgc cgcggtggct
    13321 cctggaacag gtcgaagtgg ccagcgtcga agatgtcatc cgtcaggaat gccgcaaagc
    13381 ccgccgggtc attgccgacc cgatttacgc ccggctgctg ccggatgaaa aagaaaaatt
    13441 catttccatg ccccatgaag cctattcggg acggcattat cacgccgata tgcccatctt
    13501 cgtcgggccg tccttttcgg catggctgaa aaataaattg acgtgaggag gaagtcttat
    13561 gagaaaattt gcaatttatg ggaaaggcgg catcggcaag tcgacgacgg cagccaacgt
    13621 gtcggccgcc ctggccatga tggggaaaaa agtcgtccaa gtcggctgtg accctaaggc
    13681 cgattcgacg ctgaacctcc tgcacggcca tcccgtcacg ccggtcatgg actactatcg
    13741 ggaacataca gaaggaccgg atttcgacca tatcggccgg gaaggcttcg gcggcgtctt
    13801 ctgcatcgaa acgggcggcc ctacgccggg cctgggctgt gccggccggg gcatcatcgc
    13861 cactttcgac ctcatggacg aacaggaagt cttccagaag ctccagcctg acgtcgtctt
    13921 atatgacgtc ttgggcgacg tcgtctgcgg cggcttctct gcgcccatcc gcgatggcta
    13981 tgccgatgaa atcttcatcg tcacgtccgg cgaaaagatg gccctctacg cagcggctaa
    14041 tatcatcaag gccgtcgaaa acttcaagag ccgcggctat gcccaggtca agggcatcat
    14101 cctcaaccgg cgcaatgtcg atgatgaata cgaactggtc aaggcctttg ccgatgaaca
    14161 cggcctgccc atcgtagccg atattcccag aagcaaggaa atccagtatt atgaaaataa
    14221 aggcatgacc gtcgtcgaag gcgacccgga cctgccggtc agccagattt atcgggagtt
    14281 agctcaaaag ctgctccagg tttaggaaag gagcgatatc tatggggatg aatgatacgc
    14341 cgtcaggcga acgggtccat atcggcttct ttggccgccg caatgccggc aaatcgagcg
    14401 tcgtcaacgc cgtgaccggc caggacctgt ccatcgtgtc ggcgacgaaa gggacgacga
    14461 cggacccggt ctataaagcc atggaactcc tgcccttagg gccagtcgtc atcatcgata
    14521 cgccgggttt cgacgacgtc ggcgccctgg gtgaagaacg ggtgcggcgg acaaaacagg
    14581 tcctcaacaa gacggatata gccgtcatca tcatcgacgc tgccgaaggg cggacgccgg
    14641 ccgatgacga agtcctgaat ctcatccggg aaaagaacct gccttatctc gtcgtctata
    14701 ataaagccga tctgctggag cggcccggac aggcgcagga ccatgaaatc tacgtcagtg
    14761 ccctgaaagg ggagggcatc catgaactga aagaacgcct ggcccgcctg aagcccgttg
    14821 agacagccgc gcccatcgtc agcgacctca tccagggcgg cgacttcgtc gtcctcgtcg
    14881 ttcccatcga cacggccgcg ccgaaagggc gcctcatcct gccccagcag cagacaatcc
    14941 gtgaaatcat cgacaacgac agcgccgcca tcgtcgtcaa agaatatgaa ctgcgccata
    15001 ccttggactg cctgggccag aaaccggcct tggtcattac ggacagccag gtctttgcca
    15061 aagtctcggc cgacacgccg ctggatgtgc gcctgacgtc gttctccatc ctcatggccc
    15121 ggcacaaggg cctgctggaa acggccgtcc gcggcgtcac ggccatcgaa aaactgcacg
    15181 acggcgatac ggtcctcatt gctgaaggct gtacgcacca ccgccaatgc gacgacatcg
    15241 gcacggtcaa gattccccgc tggctgcgca actatacgca gaaggatatc cgcatcgaaa
    15301 ccgccagcgg ccgcgatttc cccgacgatt tatcgggcta tgccctggtc atccactgcg
    15361 gcggctgtat gctcaacggg cgggaagtcc agtaccgcat gaaatgcgct gccgaccagc
    15421 aggtgccgat taccaattac ggcatcgcca tcgcctatat gcagggcatc ttgaaacgca
    15481 gcctcagcgt tttcccgtat ttgcagtcgc tggtgtaaaa tagtttgtag tttgtagttt
    15541 gtaggggcga ttactttggg agggattcgg atggaacaca cgcgtatcga acgcgatgta
    15601 ctaggtgaag tggatttgcc ggaaaatgcg atgtacggta tcaacacggc cagggctttg
    15661 gaaaatttcc ccttaggcca caagcggacg gctatgccgc tcatctatgc catggtgacg
    15721 gtcaagaagg ccgccgccct gacctacgcc cgcctgggtg tgcgcgaaga cgggatttac
    15781 caggccattg ccgctgcctg tgatgccatc ctggccggtc agtacgacga cgcttttgtg
    15841 accgaggccc tgcagggcgg cgccggtacg tcgacgaaca tgaacgtcaa cgaagtcatc
    15901 gccaacgccg ccttggtcat attggggaaa aagccgggcc aatacgacgt catccatccc
    15961 ctggatgacg tcaaccgcgg ccagtcgacg aacgacgtct atccgacggc cctgcgcatt
    16021 gccgccatcc gccagctgcg ggagctgagc aaggcctgtg ccgacttgca ggaagccttc
    16081 cagcacaaag aacaggccta tgagaagaca tataaactgg gccggacaga gctcatggat
    16141 gccgtcccca tgacggcagg cgaagagttc ggcgcctatg cccaggccct gtcccgtgac
    16201 cgctggcgcc tctataaaat cgaggaacgc ctgcgcatgg tcaacctcgg cggcacggct
    16261 atcggcacgg ccgatacggc gtcgcggcag taccgctacg aagtcatcga acagctgcgc
    16321 cagctgacgg gcatcggcct ggccgcggca gaatatcccg tcgacctgac ccagaacaac
    16381 gacgtcttcg tcgaagtgtc gggcctgctc aaggccttgg ccgtcaatct catgaaaatc
    16441 gccaacgact tgcgcctcat gaattccggc cctcatggcg gcatcggcga aatccgcctg
    16501 cctgcccgtc agcagggcag ttccatcatg ccgggcaagg tcaatccggt catcccggaa
    16561 atggtcatgc agtgcgccat gaaagtcatg gctgacgaca gcgccattgc catggccgcc
    16621 gctcacggcg aatttgaact caatgccttt gaaccgctca tcgccgactg cctgctggaa
    16681 agcctgcagc tcctcatccg gtcagtgcgc atcttccaga cggcctgtgt cgaaggactg
    16741 gaagtccggg aagactcgtg ccggcagcac ctccaggctt cggctgcctt tggcgcagcc
    16801 tatatcgggg ccctgggata tgatacggtg agccgcatca tcaaagacca cgaaccggac
    16861 gaagcccatc gtatcttaga ggctatggtg aaaaaataat tctttacgtg aaagaggcgt
    16921 gtcatgtcga acttatcgtt gaaactcgaa cagaaagcga aaatattgca ggtccagcgc
    16981 ctgaccatcc atatgctggc cctcaacgcc caggatctgg ccgatttttt gcaggaacag
    17041 gtgacggaca atcccttgct ggatatccgc tatcacgatg tccggccggc cggcgataag
    17101 ggcgataagg ccatcgacaa tctccgcagc cgcagtgatt ccctggaagc ccggctcatg
    17161 gcccagctcc gcgtccagtc cctgcccagg ccgcagctga tggcggcagg cctggtcatc
    17221 ggctccctcg acgataaggg ctttttccag ggcgacctgg cttccctggg cacggcttat
    17281 catctcactc cggaagacat gaaaaaaggg ctggaactcg tccagtcctt tgacccgccg
    17341 ggcattgccg ccagggatct gcgcgaagcc ctgctcatcc agacccggcg cagccgcaag
    17401 gcccctgcca agacggaagc cctgctggcg cagcattatg aagacttcct ccaggggaaa
    17461 tggcagaaaa tccaggcttc cctggccctg tctgaagcgg gcctgcaggc tatccgcgat
    17521 ttcctcaaga ccctgtccct ccagccggcc ggccagatta cccaggaaga agtctatatc
    17581 cggccggacg tggagattta ttgcgacgag aaaggccagc tggccctgcg ctcgttggaa
    17641 gagataccgg atgtctattt tcgcgatgac ttgtatgacc agtacgccgc ccagggcgac
    17701 aaggagaccc tggtctatat ccgcaaagcc cgccgtgatt tcaatgacct ggcctcggcc
    17761 ctggcctatc gccatcattc catcgaacaa gtcgtgacct gcctgatgag ccaccagaaa
    17821 gactatttct tgtaccataa gcccttgcag cccttgcggc agaaagacat cgccgaggag
    17881 acgaaactca gcacggctac ggtcagccgc gtctgccgcc accgctacgt cctctttgaa
    17941 ggccaggtat atccactgca gtcctttttg gctacggcct atgccgtcga taaagaagat
    18001 ggggccagcg tatccgataa agctatcatg cggaaaatcg cggatctcgt cgaaagcgaa
    18061 gataaggacc acccctattc ggaccaggac ttggccgaat attttgcctc tgcccagatt
    18121 tccgtagccc gacggacggt gaccaagttc cggcaaaaat tgaacatccc caacagccgc
    18181 atccggcgcc gctggaggcc gtaaaggcta aggcctcatc ttattgaaaa tcgtctgccg
    18241 cgatacgttg agcaatttgg ctatatcctt ctggctatag cctttttctt gcagttcttc
    18301 aatgagaatc tgctggatcg tcttttccag gctcttcatg ttcagctgga gggacgccgt
    18361 atccagcagg gcgttctttt gggcctcgtc cgggatatcg aataaggtat cgtagtcatc
    18421 gtccagagta tcgccaaaaa tataatagcg caaggcgacg ctgtgcagct cgcggatatt
    18481 gccccaccag tcatgctgga tgagggcttt ttttaatgac gccttgaggg gctgccggcg
    18541 atgggtgaaa ttttccagga agtaagcgaa gaggtagagg atgtcgtcag gccgttcgtt
    18601 cagcggcggc aggctcaggc gcagggtgtt gatgcggaag aacaggtcgc gccggaagct
    18661 gccggcctgg acagcggcga tgatgtcggc attgctggcc gagatgatgc ggatatccag
    18721 ggggatgacg tagtccgaac cgaggcgcat gacctgcttc gtttccagga cgcgcaggag
    18781 cttcgactgg atgctcggcg ccatgctgtt gatttcatcg aggaatatcg tcccgttgtg
    18841 ggccatttca aagaggccgg ccttgccttc tttgcgggcg cccgtaaagg acccgccaac
    18901 atagccgaag agctcgcttt ccaggaggtc tgtcggcagg gcggcgcagt tgatggccac
    18961 gaaaggaccg tgacggcggt cgctggcatt gtggatgctc tgggcaaaga gttccttccc
    19021 cgtgccgctc tcgccctgga tgaggatggg cgcgttgtac cgggccatga cttcggcctg
    19081 gcggatgacg tgcttcatgc cttcgtcgac ggtcttgatg tcctcgaaat ggtgcgtcgc
    19141 cgtcagtccc gtcttggaga gtttgtagcg gatattcttt tccaggcgct gcagttccgt
    19201 cacgtcgcgg atagtcagga tgtattggga cctactgtcc atgaggaaaa agtcagaatt
    19261 ggcgatgagc gtatacggcg gccgctggag aatcgtctcg tggaaagacg ggctcgaccc
    19321 ttcggtccag tcgggaaaga tgtcgcgcat gttcttcgtc gtcaccggaa tccccaggaa
    19381 ggacgcagcc cgttcattga tgtgggaaat ggcgccgccc ttggcataga ggatgatgcc
    19441 gtctgtgacg tgggataaga tggacgacat catggacagc tgcttattgt ccttcctttg
    19501 aaagaccagg agctcctggg cgtagagcag gacggagagc atcgtcgatt cgctgggcct
    19561 gatggaataa atcggcatgg gactgggata ggcaatctgg ggcagcaggg gacagccgac
    19621 gatggccgta tccggcgtga ccggaagctg acggacccgc gctaacagct ctaagtgcgt
    19681 cttatccagg ctgtagcggg gatagaagac gatttttttg cgaagctctt ccggacaggc
    19741 atctttgttg aacatgaagt tctcattgag cagcaagtgg atctgcttgt atttcggagc
    19801 aatagagcgc agtgtatata aggcgtcgtt ggtcgaactg aaaaggcaga gaatcggcac
    19861 gttgtcgatg gccttggtca gtatctggtg ggaaccgctg ttggtgatga tgactttggc
    19921 gccctggcgt atcaggccgc gggcctggtc gatgagatgg gggaagtcga tgatcgtgac
    19981 tgtcgtcgtg atgtcatggg cttccagttt atctttataa atgtgcagta tcgtatagac
    20041 agactgctgc atcaactcgt cagcaatcaa aaaagcgatg gtatccattt tctttcctcc
    20101 tccgtttctc tttccatcat aaagaaatac gaaataaatg taaagaaggt aaaaagtatt
    20161 ttacctaatg agcctatcgt cgaaggccct cttcgggaaa atggcttata tacctcatgg
    20221 aatgaattgg catggatatt acatatagat tagtgaatag ataatgaacc gatcgaacga
    20281 tggaggaggt atggagtatg aaaaagttat tgaccggtga tgaagccatc gccagaggct
    20341 tgtatgaagc cggcgtgtcc tttgcttcgg cttatccggg aacaccgagt acggaaattt
    20401 tggaaaatgt cgcgaaatat gatgatatgt atgccgaatg ggcaccaaat gaaaaagtgg
    20461 ccatggaagc ggccatcggc gcctctatgt ccggcgtccg ctcttttgca gccatgaaga
    20521 tggtcggcgt caacgtcgca gccgacccgc tgttcacctt tgcttattta ggcatcaacg
    20581 gcggctttgc cttcgtttca gctgacgacc cggggatgca ttcatcccag aatgaacagg
    20641 ataaccgcaa ttatgccaag tttgccaaaa tcgccatgtt cgagccgtcg gattcgcagg
    20701 aatgtatcga catgaccaaa gaagcctata aagtcagcga aacctatggc gtcccggtca
    20761 tgatacgcat gacgacccgc gtctgccatt ccaagagtct cgtcgaagaa caggaccgcc
    20821 aggaagtgcc gcccgtcctc tatcagaaac agcgggaaaa aatggatgcc gtaccggcca
    20881 tttccaagaa acgccgctat gtcgtcgaag aacgggaaga aaaattgaaa ggttattccg
    20941 aaatctgtcc cttcaacgaa gtagagatgc atgatacgaa aatcggcatc gtcgccgctg
    21001 gggcagccta tatgtatgcc aaagaagtct tgggcgacca ggcttcatat ctgaagctgg
    21061 gatttaccta tcccctgccg atgcagaaaa tcaaagcctt tgccgataag gtcgacgaac
    21121 tctacgtcat cgaagaactg gatcccttca tggaagaaca gatacgccag gccgggattc
    21181 cctgccacgg caaggatgtc atccccaaga tcgacgagct gaaccccaat atcgtatcgg
    21241 ccggcgtcct caagaaagag cagcacggcg tggacgtctc cgattcctgt ttcgttccca
    21301 gaccgcctga tctctgtgcc ggctgtccgc accgcggttt cttctatgaa ctgggcaagc
    21361 ggaaagatac ggtcatgatc ggcgacatcg gctgctatgc cttaggcggg tcggcgccgc
    21421 tcaacgccaa ggacctggcc gtctgtatgg gcagtgcttt ctccgttgct cacggcttca
    21481 gccatggcct ccagctcagt gatcagaaga agcgggttgt cgcctgcatg ggcgattcga
    21541 cgttcttcca tacgggcatc aactccctga ccgaagtggc ctacaacggc tccaacgcca
    21601 tctgcgtcat cctggacaac cgcattaccg gcatgaccgg ccatcaggac aaccccggga
    21661 cgggctacaa catcaaaggg gaaccggcga cgctcatcga tgtcgaaacg gtcgtcaaag
    21721 ccttgggtat caagaacgtc cgtaccgtca atcccctgga cctgaaggcc atgaaagaaa
    21781 cgttggactg ggcctttgcc atcgaagagg aaccgtcggt catcatcacc aagtggccct
    21841 gcgtcctcaa gaagttctcg aaagaagata aagcccagtt caacctcgac aagacgcctt
    21901 gcgtcgtcga tagggatacg tgcattggct gtaagaaatg cctttccacc ggctgcccgg
    21961 ccctgcgcta tgacagtgaa aacaagaaat ccagtattgc ccaggccgac tgcgtcggct
    22021 gtacggtctg tatgcaggtc tgcccggttc aggccatttc cagaaagggg gataaataat
    22081 atgtcagatg taacgagtgt actcttcgtc ggtgtcggcg gccagggcac gatattagcc
    22141 agtaagatcc tcaccatcgg ccttatcgaa cacgggtacg atgtcaagat gtccgaagt
//
LOCUS       DAWCYS010000007        20651 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_153_length_20651_cov_10.191833, whole genome shotgun sequence.
ACCESSION   DAWCYS010000007 DAWCYS010000000
VERSION     DAWCYS010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 20651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20651
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_20651_cov_10.191833"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4952 a         5684 c         5614 g         4401 t
ORIGIN      
        1 gggcggacca gtcgggctgt tcgaagagga ctgtcaaggt gccggaaaag aggacggccg
       61 tggcgaactg gacccaggac aacttgatgc tgttggcttt tttcaagagg aaatggtcga
      121 tgaccaggat gtgcagggcg aagaagacgg cacagcagag gaccaggaag tcgccgaaag
      181 aaacctggaa atcgccgttg atgcataaga gataaaagcc ggcaatggcc agggtgacgc
      241 agattacgat gatccgcggg actttgtggc ccatgaaatg gcccatgagg gggacgagga
      301 tgatgtacaa agccgtgatg aaaccggctt tcccagccgt cgtcatggag atgccgacct
      361 gctgcagcgt atcggcggcg cccaggacca ggccgcacca ggcaccgccg atgagggtga
      421 tgcggtccgg tttcagctgt tgcaaaatcg gcggataggt cccgtctgtc cctttatcgg
      481 taaacaacag gatgacggga atcaggacga tgatggcgat gacatcgcgg gcggcgctga
      541 aggtaaacgg gccgatatag tccatgccaa cgctctgggc gacgaaggca gcgccccaga
      601 tgagggccgt aatgagcagc agtatcgtgc ctttagcttg tgaatgatac atagcataat
      661 cctttctcta aacgaaaaat aaccagaata ttttcatttt atactgcctt gaagaaaatg
      721 taaacaaaaa tagattgccc tgggaaagag cctgcgggaa ttcaaagaag ccgtcaacac
      781 ggcggatgtc aaggccgacg aaaagaataa cgatgaaaaa ataaatgcag gccgcaggtg
      841 gcaagtggcc ggtcgtaaat acacggcctg tggccttgtg cgtaagggct gtccccctaa
      901 aaggcggccc gttagggaga tttcatcacg gactattctg gtataatagc atattttgta
      961 atatttttca caaaatatga ttgcagtcgc cgcgggaaag ttttatacta tatataaggt
     1021 tatctcatac tactattgca aagtcatgaa ggaggagact ttagaatgta caaactttca
     1081 caaattcctg aagagtataa gaagaaattg atcacggctg aacaggctgc ggctctcgtc
     1141 aaagatggcg accgcgtttc ctacggcctc ggctgcgctg ctccgtatga tatcgacatc
     1201 gaaatcggta aacgggcgaa agacctgcat ggcgtcgaaa tcgttgccac gaccgtcgtc
     1261 aaagatgaac cctatgcggc ttatctcaac agtgactcga acgaacagat ccgcttcgca
     1321 acggctcata tgaacgggtt tgaacgcaag atgtccaaag ccggccgctg ctggttcatt
     1381 cctattctct tcaacgaatt gccgaagtat tgggaaaaca tcgataaact catcatccag
     1441 gttcatccta tggaccagtg gggcaacttc aacctcgggc ctcaggcatc ggaccttcgc
     1501 ggtttgatcc gtgctgctaa gacgatcatc ctcgaagtca accagaacat gccgaaagct
     1561 ctgggctatg aaacggaact gaacctctgc gacgtcgact atgtcgtcga aggctcgaac
     1621 catcccatgc cgcagatccc caaccgcgcc ggtacggaag tcgatgacaa gatcgccgat
     1681 tacatcatcc ccatgatcga agacggcagt acgctccagc tcggcatcgg cggtatcccg
     1741 tcggctgtcg gctgcaaact ggctgaatcg gatgtcaaag atttgagcgg tcatacggaa
     1801 atgctcgtcg attcctatgt cgacctctat gaagccggca agattacggg caacaagaac
     1861 cgcgataaag gcaagatcat ctatgctttt gccggcggca cacagcgcct ctatgatttc
     1921 atcaacgaca accagatcgt cttcaacgct ccggtaagct acgtcaacaa cgtcggcgtt
     1981 gtcgccagca tcgataaatt catctccatc aacggctgca tcgacctcga cctctatggc
     2041 caggtcaacg cggaaacggc tggctatcag cagatcagcg gcaccggcgg ccagctcgac
     2101 ttcgcccagg gcgcattcgc atccaacggc ggcaagagct tcatctgcgt ccattcgacg
     2161 cgtaaattca aggacggcca tgtcgaatcc ctgatcaaac cgaccctccc ggctggcacg
     2221 gtcatcacga caccgcgttc ggctgtccac tacatcgtca ctgaatacgg tacagccctg
     2281 ctgaaaggca aatcgacgtg ggaacgcgcc gaagccctga tcaacatcgc tcatccggac
     2341 ttccgtgaag aactcatcaa agacgcagaa aagatgggta tctggactaa gaccagcaag
     2401 atttccatgt aaaaaatagt ttgtagtccg taggggcttg cacgtggcaa gcccgtccag
     2461 gatagttacc gaatccccgg cgggtcgccc acgtggcgac ccctacaact taaaaaggct
     2521 tgtcgcacgg cgacaagcct tttttctatg tcccagccaa gctggtgatt ttttccagca
     2581 tgtgttggta gaccttgatt tttccttcca gctcctggcc actgcacgga tggcagtagg
     2641 tggatttcgt catgttgttg aagaagtcca ggaccatggc gtccaggtcc ttatcgacga
     2701 tgatgtagaa cgggtcggtc cggctgttca tggccagggt gttctttttg aagaagctca
     2761 tgcggtagtt ggaatagaag gacaggttcg cttctttata ggccaggaag gccggcgaca
     2821 agtgcatggt ccccaagatg atgccctggc tctgggtcag agtccgggcg atggtcttca
     2881 agtggtctat tcgttcgcct ggcgtcaagg tgtactggac gtcggtcagg tcaatgtggc
     2941 ccgtttccag atagcgcatg aaggacggcg tcggtacgat gagcgatacg cgggccttgg
     3001 cgatgatttc ttcccaggta atgcgcaggc gttcgatatt gccggcccgg tcagccggcg
     3061 attcggcgat gagccggtcg aagacttcct ggggcaggag gtattcgatg ccgttggtca
     3121 ggaaaaagca gaaccgcgac gtttcataga aggacgtgcg gtagccgtag tcgtcgttcc
     3181 agggactctg gatgtgagtt aggagtttcg gcgtatggtg gacgccgaag cgctcgtaaa
     3241 tgtcgtggac gacggccggg tcgtcgatgt aggaacacat catcatgccc cgatggctct
     3301 ggagcgaata ctggatgacg aactggtcct tgaggatgat cagattggca tcgctcaagg
     3361 cggagaagtc gtaaaaggtg aattccgcat ccatgaagcg gttgaggacg gcgtagagcc
     3421 gggctacgta ttccggatgg cggcgtatct tctctttatc caggccgacg cggatgcgca
     3481 ggcgtttata atgggacggc acgtcgaggt aggaccagaa gccgtcgtcg taaaaatcga
     3541 agaattcccc gtagatgacg agttcctcct tgcccgtcag ggtcgggagt ttttttcgga
     3601 ataagtcttt taaaaagtgg gctaccttct gatggctcgt gatgatgcgg acgggccagg
     3661 actttttggc cttttccgtg cgcccgctct ggagcgacgt gcggtaagcc cggctcagat
     3721 agtcgctgat ggcaaagacc aggtcgggcg tatcggcggg aaacgaaaaa tagcggcagc
     3781 agtcttggcg gcgatcgagt ttttcaatcg tttcggcaaa gagtgcagcc gcatcttcat
     3841 tgacgcgttc aatggcccgt gacgatggca gcttggcccc gttgatccac ttgctgatat
     3901 acgatacgtc gtaaccgatg tattcggcca gggtcacgga cttcattttc gtgaatttca
     3961 tgaatttttt caaagcaatc gggtaggttt ctacaatcat gcacattccc cctctatatg
     4021 gcagccatct tttaatatat cccctcagac ggctatttat cttattttaa cacaaaacga
     4081 tgccataaag aacaaataaa tttaaggaat aaatgatgaa ttcatgaaaa tttcaaattt
     4141 tcctgttttt ttcaagatag gctgggacta tactaaggcc atgcttgagg acatcaagcg
     4201 gagcaagaat ttagacatca tgattatagg aggtggacgg acgtgagacc ctttgttttt
     4261 cctctttcac agcatatcgg cgctccatcg gtcccagtgg tccagcctgg agacagggta
     4321 cagcgcggcc agtgcatcgc tgaggctggg gaaggattag gaagcccggt atatacgagt
     4381 gtatccggga cggtcctgga agtgacggat gatgccgtca tcgttcgagc tgacgacgtc
     4441 cagccgaaag agtacgtgcc tcttacggca cagactccat tggattatat ccgccaggcc
     4501 gggatcgtcg gcttaggcgg cgccggattc ccgacgtatc agaaactggc cgttcccttt
     4561 ttgaacggcg gcgtcgtcat tgccaatgct tcggagtgtg aacccgtcct gacccataat
     4621 atcaaggcca ttgagcggga ctgccgcccg atgatacggg gcctgcagct ggccatggac
     4681 ctcgtccatg ccgataaagg cgtcattgcc atcaaggcca tccacggcga cgccgtccga
     4741 gccttacagc aggccatcgc cgctgacgac cgcctggccc ttcattttct gcccaatgtc
     4801 tatcctgtcg gcgaagaacg ggctgtcgtc cgggaagtcc tggggacgct cctcgatgcg
     4861 ggcagtctgc cgtcgaaagc cggcgccgtc atcatcaacg tggaaacgct ccagcgcgtg
     4921 gccgaagccg tcgatgacaa gaaaccgctc atcgataaag acgtgaccgt agctggcaag
     4981 ctgaacggtc cggaagtcca ggtcttccac gacctgccct tgggcatggc cgtcaacgat
     5041 gtcctggaaa aggccggcgg cctgggcaaa gaatatggcg agctcatcat gggcgggctt
     5101 ttcatgggca ggcggacgag cctggacgca cccatcgtca agacgacagg cggcatcatc
     5161 gcggcagaaa cgttcctgcc agggcctgaa aaaatcggcc ttctcgtttg tgcctgcggc
     5221 gccgatgaaa agcgcctccg ggaaatagcg gcttccatgg ggtcggccgt agccggcgta
     5281 gccttttgca agcaggccca tgaagtcaaa ccgggcgtcc gcaaatgcga caatcccggc
     5341 cactgtccgg gacaggtcca gcgggtcatg acgctgaaga aagaaggggc ccaggcccta
     5401 ctcatcagta actgcacaga ctgcaccaac acggtcatgg cctgtgcgcc gcagcttcat
     5461 ctgccggtct accattgtac cgatggcgcc ctgcgggcag tccatcataa attaatccgc
     5521 cgcatcaagg cgtaactcat actacacagg gggagtgatt gtatgtctat taccaaagat
     5581 accttacaga aacaccttac agatcctgcc gttttctgct gccgccgggc caaggggacg
     5641 gtcatcggag ccgatgacct ggaagacccg acgctgttcg atgacatggt cgatgccggc
     5701 ctgctcacgc tcagtgacga tggcctgacc atcgaagaag tcctgggcag cacgctcttg
     5761 caggatgtcg aagccttgac gccgattact aaagatgtcc tggataaagt caatgccaag
     5821 tccgtcccgg ccgaagcacc ggcagaaccg gtcaaagcct cgccagctgc accggccgcc
     5881 ccgacagccg caccgtcggg aacgattcac ttgaagatag ataaactgga aggcctgagc
     5941 ctggatatcc cggccggtac catcctgggt gccgctggcc cgctgccggc agggccgacg
     6001 gaaaagacgg gcgaaaagaa ggtcatccgc aagctcatca agaaacacat caaaatcacc
     6061 gatgtcgaaa tcagcgacaa gacggccatt gaagacggga agatcttcat cgacgggtcc
     6121 atcgtcgaaa aggccgtcaa agaggacccg ctctgcgtca gcatgagcct cgacgtcatt
     6181 tatcccgata aacgccacgt ctacaccgat accatcatgg acgtagcgcc cattgcgacg
     6241 aaagtcgaag gcgaactcgg tgaaggggtc accaaagtcg ccgacggtgt cgtattcatg
     6301 ctcaccggcg tcgacgaaga cggcgtccag atccatgaat tcggttcgtc tgaaggcttt
     6361 ctcgatgaaa agatgtattt cggccatccg ggctgcgccg atgaaggcga catcatcatc
     6421 cgctgccatg ccgtcatcaa acgcctcacg ggcatgaccc gtccgggacc ttttgccgct
     6481 cataaatgtg aagactacat catccaggcc gtccgcaatg aattgaagaa atacgacggc
     6541 gaagtcgtcc gcgaagaagt ctgcgaagac ctgcgccgca aaggcaatcc ccgcgtcgtc
     6601 ctggtcaagg aaatcatggg tcagggggct atgcacgaca acgtcctctg cccgaacgaa
     6661 ccgtgcagcg tcatgggcgg ccagaagaac gtcgacctcg gcaacgtacc gattgtactg
     6721 acaccgaacc aggtccgcga cggttccatc catgccctga cctgcatcgg gccggctacg
     6781 aaagaaatga cccgtcatta catccgtgaa cccttggtcg aaggcctggc caatgacgat
     6841 gaactggacc tcatcggcgt cgtcttcgtc ggctcgccgc aggtcaatga cgaaaaactc
     6901 tgggtttccg accgcctagg ctcgctcctg gaaagcctcg acatcgatgg ctgcatcatc
     6961 acgaccgaag gctttggtaa taaccacatc gacttcatcc agcacatcgg ccaggctggc
     7021 aagcggggca tccccgtcgt cggcgtttcc ttctgtgcct accagggcca gctcgtcgtc
     7081 ggcaatccct atgccgatgc catggtcgaa gaaaatatgg accatggcgg ctttgaaaac
     7141 gacgtggccg gctgctcgtg cgtcactaaa gaagtcgcag cccgcgctat ccagatgctc
     7201 aagaataaga tggccggcgt ccccattgcg gctgcaccga agaaatggga caacgccgtc
     7261 atcaacgcca acaataaact cctcggtctc cctgaaacgg tcctcctcga taaagggacg
     7321 ctccactgat ggccaaactc gattatcagc tcaacccggc actcatggaa gggacggtca
     7381 ccaagaccgc tgcggacgac gtggctctca acctgagagg gagactgggc gtgatccacg
     7441 ctgtaccgtc tttgttcctc caccagcccc gggaaggccg gaaattccga ttctatttta
     7501 gttacatgca gatcgtagag accccgctcg attacgatta tgggccgctc gaaaaagacc
     7561 gggaattcac gccggtcctg gccggcggtg tcctcagcga agtcaacgat acggccatca
     7621 aagccgactg tctcggcggc ctggctacca ttgccgtacc gcggcgctgg gtctttaccg
     7681 atgtcgccct gaaagaggga gaatatactg aattttacat cagccccatg gcggctgtag
     7741 atgaactata ggaggggtag ttatgaactt aacgactgta gaagggatgc gctcggaaat
     7801 cttcgttccc gttacaccga aaccggtatt tactgaatta aagaaaccac tgagtgaatg
     7861 taaagtcgcc ttcatcacgg ctggcggcat ccataaaaaa gaccagacgc cgttcaatac
     7921 gtccggggat ttctcgtacc gcgtcatccc cttcgacact ccatcgtcga acctgatggt
     7981 cacccacgga ggcttcgaca actcggatat caacaaagac gtcaatgcca tgttccccat
     8041 cgaccgcctg cacgaactgg ttgcagaagg cttcatcggc tccctgccgg aagaaacgta
     8101 tactttcatg ggcggcggcg gcaacgtcga aaaattccgc aatgaaacgg ggccggaaat
     8161 tgccaagaag ctcaaagccc agggcgtcga catcgttctc tgcacgggcg gctgcgggac
     8221 ctgccaccgt tcggcgacga tcgtcacccg ctgctgcgaa gccgaaggca tgagctgctg
     8281 cgtcatcgcc gccctgcctc ccatcgcccg ccagcagggg gcaccgcgca tcacagcgcc
     8341 gcacgtccct atcggctcca atgccggcga accgaacaat atccccatgc agacggctat
     8401 cctcaaggaa tccctggaat gggtccgcga ctgcccgagc tataacggcc tgaaagtcct
     8461 cccctatgaa taccgccaca atgtgtaata acggcacgta ataaaaatat aataaggtta
     8521 aacgttgcat atgtgctaaa atgatagtat aataaatttg ttattaaaga tattaaacaa
     8581 ataagaatat aaacttaaaa taaacgatgg cgtttatctc cgtgagagat gaacgccatt
     8641 gctcgtttta aggaatgaaa ggggagattc gatgtggcat gctaatcagg agcgcctgat
     8701 ggaaaaactg aggggagaag aacaattcct ggtcatcgat tcgcatacca tgggagaacc
     8761 gacgcgtatc gtcgtcaatg gatttcctca cgcgccaggc aggacgatga tggaaaagaa
     8821 gcagtacatc gccaagcact acgatgccta tcgcaaggcc atcttgctgg agccgcgggg
     8881 ccatacgaat atgttcggcg ccatcctcat gacgccggtc cacgaagaag ctgatgtcgg
     8941 cgttgtcttc atggacagcg gcggctacct gaatatgtgc ggccacggtt ccatcggcgt
     9001 ggcgacggtg ctggtcaatg aacagatgat acccgtccat gaaccctata cggacatctg
     9061 cctcgatgcg ccgtcaggcc tcatcaaggg ccacgtcaaa gtcgaagcag gccgggccgt
     9121 agaagtgacc attaccaacg tgccctgctt cttgtaccgg acaggggtca cggtccctgt
     9181 cgacggctta ggccacgttg ccctggacat cgcctttggc ggcagtttct tcgccctcgt
     9241 cgacgccgat gctttgggga tgcgcttaga gcctgaaaat atccggacta tcatcgacac
     9301 gggcatgaac atcctccggg ccgtcaacaa tcaggaagtc gtagcccatc cggaactgga
     9361 catccacagc gtcgatttag tcgaatttta cggtcaggcc gatcggtccg aagcgaccat
     9421 gaagaacgcc gtcgtcttcg gcaactacca gatcgaccgc agcccctgcg ggaccggcac
     9481 gagtgccaaa gtggcgtcgc tgtacttgcg ggggaaattg aagaaaaatg aactattcgt
     9541 ttatgaaagt atcatgggaa ccttgttcaa agcccgcatc gtagatgatg ccgaagtcgg
     9601 cggcatggcc gctgctatcc cgcagattac aggcagtgcc tatattaccg gcatcaactg
     9661 cctggtcctc gatgagacgg acccgttccg ccacggcttc cagattcaag cctgaatata
     9721 cagttccggt catgagacct gaatgataga ttccagtcat acatgtgaag aggggtgcct
     9781 gattatgatg atgaacagta aggtaaaaaa agcaatctta gccggcctga gtgccgtcgt
     9841 atccatatct gtcttagctg gctgcggcgg aggtagcggc agcagcgata aggccagcct
     9901 gaaagggaaa gagctgaaag tagcgaccgg gatctatgcg ccctttgctt tcaaagatga
     9961 aaacagtaag ctcgtcggct tcgacctgga cctgctcgat gccatgtcca agaaactggg
    10021 ctttacctat aaagtcacgc cgacggaata tgacaacatg ttcatggccg tcgccaacgg
    10081 tgagtacgat atcggcatgg gccaggtctg cattaccgaa gaccgcaaga agaagatgga
    10141 cttcaccgaa ccctatgaaa acgcaggttt gcagttcgtc gtccgcaaag atgaaaacct
    10201 tacgtctgta gatcagttga aaggcaagaa aatcgccgtc gaaaagggga cagctgccca
    10261 caaatacgta acggaacact gtaaggattc ggaaatcgtc gtcttcccgg ctatttcagc
    10321 agccttcatg gaagtcgaac agggcaaggc cgacgccctc atgcaggata aaccgaatgc
    10381 cgtctattat ttgaagaagc atcctgaaag cggtctggcc ctcttgggcg atgaaacggc
    10441 caagattccc gatggcttcg ccctgcctaa ggacagcccg tataaagctg aattcgataa
    10501 ggccctgaaa gaattgcagg aagacgggac aatcaagaag ttagaagata aatggctgaa
    10561 tgtctaacgg cagcagccag tcagcagtgg cccggcaaga gtcgtacttt tgccggactt
    10621 cttgctataa ggggtctttt attaaggggg gaatcttatg tttaactggg aacacttgga
    10681 catcatcgtc ggtgttgtta cgaaaggcct gttctggact gtcgtcattt ccgtgacgag
    10741 tatcatcatc ggctatttca tcgggctggc tacggggttt tcccgggccc gcaatatacg
    10801 catcctcaag agcatcagcg ctttctacgt ctggatcatc cgcagtacgc cgatcctggt
    10861 ccaggccttg tacatctatt tcgtcatgcc aaaactcttt ggcatccatt tatctcatga
    10921 agcggcaggc atcctctgcg tttccctcaa tgccggggcg tatatatcgg aaatcgtccg
    10981 cggtgccatc ctggaagtgc ctaaaggtca gtgggaggcg ggcatgtccc tggggatgac
    11041 gaagctgcag atcgtcatga agctcatcct gcccatggcc ttccggacca tgctgccggc
    11101 cctgggcaac cagttcatca ttaccgtaaa ggatacgtcc attttgacgg tcatcggcgt
    11161 cgccgaaatg acctatcagg ctggccagta cgcttcgtcg aactttgaat tcgtcgaaag
    11221 ctataccgtc ctggccgtat tctacttagt cctgatttcg ctgctgaccg tagccgtcaa
    11281 tatttgtgaa cagaagatgg gagctgatat aaaatgatgc gaattgaaca tttaaagaaa
    11341 cagtatggtg ataacctggt cctgaatgac atcagcctgt ccctggacga tgaagtcacc
    11401 tgcatcctcg ggccgtccgg cgccggcaag agcaccttcc tgcgctgcct gaacctgctg
    11461 gaatcgccga caggcggcga catcaagtac aacggtcaga gcatcctcca gccgggctac
    11521 gatatccgca agatgcgcga agaagtgacc atggtcttcc agcactttaa cttatttccc
    11581 ctgcgcaacg tcgtcggcaa cgtcatgctg gccctgcaaa gcgtcaagca catgaagaaa
    11641 gccgatgccc acgatattgc catggaaata ctaaaaaagg tcgacatggc cgataaggcc
    11701 gaaaagatgc cggatatgct ctccggcggc cagcagcagc gcgttgccat tgcccgcgcc
    11761 ctggccatga atcccaagat gatcctcttc gacgaaccga cgtcggccct ggacccggaa
    11821 ctcgtcggcg gcgtccttga cgtcatgcgc gacttggccc ataacggcat gtccatggtc
    11881 atcgtgaccc atgaaatggc ctttgcccgc gacgtatcag accgcatcgt cttcatggcc
    11941 gacagcatcg tccaggaaga tacgacgccg gctgaattct tcagccaccc caagacggaa
    12001 cgggcccgca atttcctgag ccgcttctta tcgtaaggga gggagtacga tggaaaaatt
    12061 catcattacc atcaaccgcc agttcggcag tatgggccgg cccattgcca aagaaatggc
    12121 cgaagccctg aacgtcgaat tttatgaccg cgacctcatc gaagctgcag cgaagaagat
    12181 gaatatgtcc ttgtctattg ccagcgacct ggaggaatct gccaggccga accgcttctg
    12241 gcgcatgtgc ttcccactgg ggcacgacga atcgcggaaa caggacgaac tcttccaggt
    12301 ggaaaagaaa atcatcctgg acctggccga tgccaagtcg tgcatcatcg tcggccgctg
    12361 ttccgattcc atcctgaaat gttccaagca cttgctgcgc atctttatct atgcgcccta
    12421 taaagaacgc ctcaaaaact gcgtcgaacg cctcaagatg gacgaacgga cggccaagga
    12481 aatgatacgc aacgtcgacc gggcccgcga tgcctaccag gtgcgctacg ccggctatct
    12541 gccaggcgac ttccaccata cggatctgct catcaacagc agcttcctcg gcgtcgaagc
    12601 gacggcccag tatctgaccg aactggtact caaaaaatac ggttcccatc atatcgaaga
    12661 agtatgaaaa ggctcccgcc agggacatgc tgtcttgtta ttgacaaatg agattaccta
    12721 ttgcatgata gtgataatac taagaggtat cagacaaagg agatgtctta tgggcgggag
    12781 caaaatctgt tatatctatt tcgtgctgag tcaggccatc tggggcgtac tgccggcctt
    12841 ctggctgctg ttgcggcagc tgccgccgct gtatacgctg gcttcgcgga ttgtctgggc
    12901 atcggtcttc tgctttttac tgatattgca gaaaaagctc ctgcccgacc tgaaaggggt
    12961 ccgacaggag cgctggcaat ggccttatat tgccggggcc tgcatcttta tcaccctgaa
    13021 ttggggggct tatatttacg ccacgacaca gggcttcatc ctccaggcca gcttggccta
    13081 tttcatcaat cccatcatcc tcgtcttctt tggtggtctg atcttccatg aacggctgcg
    13141 gccggtccag aaactgtccg tcgtcgtcgc cgctaccggc ctggtcctgg ccttctgcct
    13201 gtacggcgtc gtcccctggc tgtcgttctt catctgcctg agttgggcga tatacagcat
    13261 gttgaaaaag aaagtccgcc tggacagcca ggtttcggtc ttcatcgaat ccttttccat
    13321 ggtgcccctg tccctgctgt tcatcgcctt cagcgaatat catggcatgg gggctgtcgg
    13381 gactttccag ggctggcagt ggctgctcct gccggcgacg ggcatcgtca cggccattcc
    13441 gatgatgctc ttttcggccg gcgtcaaagg cgtccctatt acgacaggcg gtatgctcat
    13501 gtacctctcg ccgagcatca ctctggtcat cggtctcttg agcggcgaaa ccatcacgcc
    13561 gcccctgcgc ctgaccttct gcttcgcttg ggttgccgtc gccctgtata tgtacggcct
    13621 ttaccagact atcaaagggc tccgggaaag cggataccat taggtacgat ccttgctcct
    13681 gaatgacgct gtaggggctc gcacgtggcg agcccgctcc aggatatgta cgatttcccg
    13741 gttagtttgc taagagaacg attcaaatac ccggcgggtc gcccacgtgg cgacccctac
    13801 agaaaaaagg gtgtcgtacg aggtttgtgc caccatcctc taacccctaa cccctaacaa
    13861 cttgaaagga gctgtcgcga tgcgacagct ccttttgtat cacttacttt caaaaaatgc
    13921 tttgtatgcc aggtagccgc tgtaggcatc ggctttggcg acgatttcta agggagcgtg
    13981 catgctgtgc atggctacgc cgcagtcgac gacgtcgcag ccccagtcgg ccatgatgta
    14041 ggcgatggta ccgccgccgc cctggtcgac cttgcccagt tccccgacct gccagggaac
    14101 ttctttttcg ttgaagatgc ggcggatctt ggccatgaat tcggcgttgg catcgttgct
    14161 gccggccttg ccgcgggcac cggtgtattt gcacaggcag ataccgtagc cgagcttggc
    14221 cgaattctgt tcttcatagg cgtcggcaaa gaccggggca aaggcggcgc agacgtcggc
    14281 cgacaggact tcgctgttgc gcagggtccg gcgcagccag atttcttggt catggccctg
    14341 gagggacagg agttcggcga taaagtccgg gaagtacgac gattggacgc ccgtgttgcc
    14401 gacggaaccg atttcttctt tatcggcaaa gagggcgacc tgtgtctttt cccccggtgc
    14461 ggcatcgagg atggcggcca ggttggcata agagcagacg cggtcgtcat ggccgtggga
    14521 catgatcatc gaccggtcca ggccgacgtc gcggctcttg acggccggga tgatttccag
    14581 ttctgcactg gtgaaatctt cttcttcaat accgtatttt tcctttaaca aggtcaggat
    14641 agccggtttc gtcttttctc cttcgccgtt cgtgccggcc aagggcatga gcatttcgcc
    14701 gctgatggct tcagagagct ttttggcggc ctggtccttg cccaggtggg gcagaaggtc
    14761 attgatgaac aggaccggat cgcccgggtc ttcgccgata gagacgtcga cgcggctgcc
    14821 atcggcttta tagacgacgc cgaccaggga caggggcagg cagacccatt gatatttcag
    14881 caagccgcca tagtaatggg tcttgaaata gacgatttta ttcttttcga cgacaggcat
    14941 ggccttgaca tccaggcgcg ggcagtcgat atgggagccg acgattttca gccctttttc
    15001 gatgggggcc gagccgatga cggctaagat gaccgatttc cctttctggt cccaatagac
    15061 cttgtcgccg gccttcaggc tggtgaagtc agccagggga tggaacccgg cgttttcagc
    15121 ctggcggatg atttctttat tggccagccg ttctgtccgg gctgtgtcca ggaactgacg
    15181 gtaatggacg ctgtacgctt ccatctggcc gcgctgttcc ggtgagaagc gggaccagac
    15241 gctgttttct gtatcaaatg ctttcatgat ataagcctcc tctgtaaatg atgatgtgtg
    15301 acactgcatc cttattataa cgcacttatg ggagctgtac tagttcttga aaaagacagg
    15361 ggaaaccgtt gtatttcttt atattgtgtg gtattattaa tcatacttta taaataataa
    15421 ttcatacatg ggagggtttg ccatgatcga taaaatgact atcaaagact tagtgaagcg
    15481 gactgtgcct gatatttgtg ccatgcgccg tcatatccat gaaaatccgg aattatccgg
    15541 cgaagaagtg gaaacggctg cctttatggc agccaagctg cggtctttcg gcatcgatgt
    15601 acaggagcac gtcgccggaa cgacggcggt cctggggacg ctcaaaggcg gcaagccggg
    15661 gccggtcatc gccttgcggg ccgatatgga tgctctgccc atgacggaag aaacaggctt
    15721 gccctttgcg tccaatttcg tcggcaagat gcacgcctgc ggtcatgacg gccacatgag
    15781 catcctcctc ggtgccgctg aggtcctgag tcaggtgaaa gacgatatcc ccggcacggt
    15841 cgtcttcatc tgccagccgt ctgaagaaaa atcgcctgtc ggcggagcca agggcctggt
    15901 cgcctccggc gtcctcgacg gtgtcgatgc cgtttacggc cttcacgtct ggccgacact
    15961 gcccagcggc aaaatcggtg tcctgcccgg gccgatgatg gccgcttcgg accatatccg
    16021 catcgtcctc aacggcaagg ccagccatgc cgccatgccg cataaaggcg tcgacaccat
    16081 cgtcgcagcc gcccagttcc tgacggcggc ccagaccatc atcagccgcc gcgtcaatcc
    16141 cctctatccg gctgtcctga cttttggcaa aatcactggc ggcacgcgct acaatgtcgt
    16201 cgccgatacg gtcgaaatcg aagggacctg ccggacgtac catgcggagg cccaggatgc
    16261 cgtcgaagcc catctccagg atacgctcaa aggcatcgat gccatgtacg ggacggtcag
    16321 ccatctcgac tatgaacggg gctatgcggc tgtccgcaat acgccgcagc aggcggcttt
    16381 cgtcgctggc gtagccaaag actgctttgg cccggacgtt ctggcagctg tcgacgaacc
    16441 ggccatgacg gccgaagact tttccggtta tctccagaaa ttcgatggcg gtttcttctg
    16501 gctcggtgcg accaagcccg gcgacacggt ttatcccctg cacaataccc attttgccgt
    16561 cgatgaagcc tgcctgccag tcggcgtcga actgatggtt tccctggtcc tggccaaaat
    16621 gaaagaaaaa gcataattat ttttgaaaaa atgcaactta aaaaaggaat ctgccccctt
    16681 catctcgaat atatataata ttaactgaga ttccatagga ggcgattgtt ttatgaaaga
    16741 atacacaagt gataaaattc gcaacgttgc tgtactttcg catgatggcg ccggcaaaac
    16801 ggctgttgtt gaatcattac tgcttgcctg cggtgctgtc gatgctgttg gcgtcggtaa
    16861 agacaacaag catattatgg actatgaacc ggaagaaatt aagagaaacg ttacgatcca
    16921 gctcggcatt gcgccctgcg aatgggaagg ctacaagctg aacttcctcg atacgccggg
    16981 gtattccgaa ttctgcggcg aagtccgcgc tgccctgcgt gccagtgacg gcatcctgct
    17041 cgtcgtttcg gccgaatcgg gcgtcgaaat ggatacggaa cgggcctggg actacggcgt
    17101 cgaattgaaa ttgccgcgca tggtctatgt caacaagatg gatgcggaac acgccgattt
    17161 cttcggctgc ctggaaaaat tgcgcgccgt cttcggcaaa tccatcatgc ccctccagat
    17221 ccctatcggt gaaggcaagg atttccgggg catcatcgac gtctgcaaga tggtagcctg
    17281 ggaatggaat aacggcgaat gcacggaaat caaggtgccg ccagaataca tggccaaagc
    17341 ccgtgaagtc cgcgaaatgt gcatggaagc cgctgccgaa ggcgacgacg aattgctgga
    17401 aaaatacctc aacggcgatg aactgaccat cgaagaactg cgcaaaggcc tgcgccaggg
    17461 catgctgacg ggccgcgtct gcccgctcat gtgcggcagt gccatcaacc atatcggtac
    17521 ggcagaactc ctgcgccgta tcatcaccta catgccggat gcttcgcaga aagtcatgat
    17581 gggcacggat aagaagacag gcgaaccggt cctggtctat cccaacaagc ctttcacggc
    17641 cttcgtcttc aagaccctca tcgatccctt tgccggcaaa ctgagttatg tccgcatttt
    17701 ctcgggcgaa ctcaaggaag gggacaagtt atacaatata tcccaggata aagaagaaaa
    17761 gatgggcaaa gtcctgaccc tggtcggcaa ggaacagatt cccgtcccgg cggctattgc
    17821 cggcgacatc gtcgtcctgc cgaaattgcc gaatgcccgg actggcgata cctttgccgc
    17881 tcctgatttc cccgtcgtct atgaccccat ccgcttcccg aacccgctgc atacggtagc
    17941 cctggtcccg gaaaagaagg gcgaagaaga aaaattgatg aacgccctcc tgcgggtcag
    18001 cgaagaagac ccgacgtgcc aggtcgaaaa gagcaccgaa ggcaagcagc tcatcgtccg
    18061 ctgcatgggc gatgtccacc tcgaccacat cctgaccaaa atcgaacgga aatacggtgt
    18121 caaagccaaa caggaagacg tttacatccc gtatcgtgaa accatcaaga gcaaggcgac
    18181 agccgaaggc aaacacaaga aacagagcgg cggccacggt cagttcggcc acgtcttcct
    18241 ggacatcgag ccgctgacga caggcgaacc ctttgaattc gtcgataaga tctttggcgg
    18301 cgccgtaccg aaacagtaca tcccggccgt tgaaaaaggc gtccgtgaaa ccttggaaaa
    18361 aggcctcatc gccggcttcc ccatgatcaa cgtcaaagtt accctgaccg atggttccta
    18421 tcatcctgtc gattcgtcgg aaatggcctt caaagtcgct gccgctcagg ccctcaagaa
    18481 agccgtacct gaagccaagc caatcctcct tgaaccgatt tacaacgtcg acgtcgtcat
    18541 ccctgaagat tacatgggcg atgtcatggg cgacttcagc agccgccgcg gccgcatcct
    18601 cggcatggaa caccatcgca accgcaaagg catcatcgtc gtcaaagccc aggtcccgct
    18661 ggcagaaatg aaagactacg tcaccgtcct gcgctccatg acccagggca aaggcacgtt
    18721 caacatggaa ttcttcgact acgaagaagt accgaaaaaa atcatggacg aaatcatcga
    18781 aaaaagacaa agtgaataag actagtggct agtcgttcgt agtttttagt ttgtagaaat
    18841 gggctttaag tttttaaaca tccgctaacc actaacaact taaaagggct tgtcgcatgt
    18901 gcgacaagcc ctttttttta cgaaccacga atcacgagcg atgaaccacc aggcgtcacg
    18961 gatgtagggg cttgcacgtg gcaagcccgt tccagactat gagtgatttc ccagctcgga
    19021 gcgaatgcca cccaaggtcc gtcgggtcgc ccacgtggca tatgccaaaa cggcatatac
    19081 ctgtttacct aaacgccttg ccagggcgcc ttcagcgtcg tataataaga atataagcaa
    19141 acgtaaaacg tcgtactaaa gaaaatacat acagatgaaa gggtggtttt tatgtcccaa
    19201 tggacagata tgtacagaca gaaattaatg acgccggaag aagcggtaaa agtggtccag
    19261 tccggagatt gggtcgatta cggcatgggc acgacccagc cgatcctcct cgaccaggcc
    19321 ctggcagcgc gccgtgatga actgcaagat gtcaaagtcc gcatgtgcct gagcgtggct
    19381 ccgcggcaga ttattgaaca ggacccggag cgcaaggcct ttacggccat gaactggtat
    19441 atgagcggct atgaccggaa gaagtgcgcc cttggccaga tgaatttcat acccatgtgt
    19501 taccgcaaca agccgtccat gtaccgcgac ctcctcgacg tcgacgtagc cctgattact
    19561 gtcggtccca tggataaaca cggcttcttt aatttcggcc cggccgtttc ggcaacggaa
    19621 gccattacca agaaagcgaa gaaagtcatc gtcgaagtca atgaagccat gccccgcgtc
    19681 ctcggcggcc gcggcgaatg tatccatatc agcgatatcg acggcatcgt cgaatacggc
    19741 aatcatcctt tggcgaccat tccctttacg acaggcgacg atatcgatgc ccagattgcc
    19801 aagaccatcg tccagcagat tcccgacgga gcgacgctgc agctcggcat cggcagtttg
    19861 cccaacacga tcggcgcctt attggccgaa tcggacctga aagacctggg cgtccatacg
    19921 gaaatgctcg tcgacgcctt ccgactcatg tatgaacacg gccagctcac gaaccgccgc
    19981 aagactttct gccgcgataa gataagctgg gccttcgccc tggggacgca ggacctctat
    20041 gactggatgg acgacaaccc cttcctggca gcttatccgg tagacgtcat caatgatcct
    20101 ttcatcattt ctcagatgga taatttcatt tccatcaata actgcatcga catcgacctc
    20161 ttcggccagg tatcgtccga atcagccggt acccatcaga taagcggcag cggcggccag
    20221 ttggacttca ccgacggcgc ttaccgctcc cgtggcggca agagcatcat cgctttgcgg
    20281 tcgacgttcc acaacaagaa gaccggccgc gatgaatcgc gcatcatccc gacgctggcc
    20341 ccgggcacga cggtcacgga cccgcggtcc caggtcaact gggtcgccac ggaatacggc
    20401 atagtcaacc tcatgggcgc ttcgacatgg gaacgggccg aacgcctcat cagcatcgcc
    20461 catccggctt tccgcgaaga cctgatcaaa gaagctgaaa aaatgaagat ctggagatac
    20521 agcaataaga aataaaggga tacgctatta ttctggtaag tgttcccacg gtatgctctc
    20581 gttcgtcgta ggtcgttcat cgtaggtcga aaattaaaaa gtaattacga tgaactgcca
    20641 gcccctaacc c
//
LOCUS       DAWCYS010000008        20445 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_159_length_20445_cov_7.824522, whole genome shotgun sequence.
ACCESSION   DAWCYS010000008 DAWCYS010000000
VERSION     DAWCYS010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 20445)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20445)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20445
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159_length_20445_cov_7.824522"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    19084..19093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4818 a         5507 c         5250 g         4860 t
ORIGIN      
        1 ctttcttgcc agcctgacgg gagccataag ccttttcaaa gacgccgccg acgggcatga
       61 cctggccctg gagggaaatt tccccggtca gggccatgtc ctggcggacc ggcttgcctg
      121 tgatggccga gatgagggcc gtcgtaatgg cacagcctgc cgacgggccg tcgatattgc
      181 cgccgccgac gaagttgatg ttgacgtcgt aatcggacag ttccttaccg gtcatgcggc
      241 ggacgacagc ggctgcgttg aacatggagt ccttggccat ggaaccggcc gtatcgttga
      301 agcggtaata ccccttcccc ggcgcccgcg ccggaaaggc gacggcttcg atttcaatgg
      361 ccgagccgag gtaaccggcg acgccgaggc cgaagacctt gccgacgaca ggcgtatcgc
      421 tggccttttc cgtcacataa ggcacgaggc ggctgatctg ggcgacgcgg cggacgtggt
      481 cgcaggtgat gcgcacgtgg tccttggagc ccgatttaaa gacagacagg ccatatgtat
      541 cggccaggat gttgacggcc ttgcggcctt cgatggtgaa ctcactgatc aatttggcca
      601 cgccgtcttc cagttccgcc cccagctggg cagcggcatt ggtgacaatc tgttcgatat
      661 ccgacgggat gaggggttca aagaagattt cagcgcagcg cgaccggatg gccggattga
      721 tttccgacgg gtcccgcgtc gtcgcaccga tgaggatgaa gtcggccgga gccccttcgg
      781 caaagagttt cttgatatac ttcggcacat tcgggtcgtc ttcgtcgtaa taagccgatt
      841 caaaacggac ccgcttgtct tccatgactt tcagcagctt gttgagcagc atcgggtcca
      901 cttccccgat ttcgtcgatg aagaggatgc cgccatgggc ttcggtgacc aggcccggct
      961 tcggttccgg gatccccgta tcggccaggt cgcggcgggc gccctgataa atcgggtcgt
     1021 ggaccgaacc gatgaggggg ttggtcatat cccgcgaatc ccagcgcagc gtcgtcccgt
     1081 ccgtttcgat aaagggcgca tcagggccaa acggtgtaaa gggcagcttc ttggcttcgt
     1141 cgaggacgat gcgggcagcc gtcgtcttgc cgacgccagg cggtccgtag aggagcaggt
     1201 gctgcggata gacagacgat aacttgctct gcatggcttc cacggcccgt tcctggccga
     1261 caatagcccg gagctgtttc ggccgtaccc gttccatgac cgattccgta agatggacgt
     1321 tttccaggtc gtccaattcc ttgagcttgc gcttggtctg cggcgtttcg acgtcgtgca
     1381 gttcttcctt gatgagttcc atcttgattt cccgcacgta ttcctgctgt ttttcatcca
     1441 tgcgctggct gattttcttt tccagccgtt cttctacggc ctgacgggcc agcatctcag
     1501 ccaggaccat ttccagttca ttgaggacgt tgatgacttc atcgtcatcg ggtactttat
     1561 catacgagac gtcttcatag atgaggcgct gcagtccgac caggcgctgg cgcgggtctt
     1621 cggaatggat gtacgacaag gcagagtact tactggcccg gaggaccatc ttttcagacc
     1681 ctataagacc agtaaaagcg ccataaagca cattaacctg acggcggagt tcttcctccg
     1741 ccgtcggttc gaggtactta tggcgctgta ataatttgtc caataattct ttcattgtat
     1801 ccccttatgg cttatgatca gccttctaca acgtgtattt tgatgacact ggtaatcgtc
     1861 ggatggacgc ggatggtaac atcgtaatcg ccgagcgatt taatcggatc cttgatttct
     1921 acttttttct tgtccaggtc gatatcgtac tgggccttgg ccgcttcgct gatagtcttg
     1981 cccgtaatgg caccgaagac tttgccgcct tcgccgactt taacgggaat ggtcagttcg
     2041 actttcttca gctggctggc caggatgacg gcttcatccg aagcgacctg ggccttatgc
     2101 ttggcagcgg cttttttctg tttggcatcg ttgaggtttt ccgatgtgcc gggagcggct
     2161 aatttacggg gcaggaggaa gttgcggcca tagccttccg atacttcgat gatgtcccct
     2221 ttcttaccga gtttctttac atcctgtaat aagattactt tcatttttca tcaatctcct
     2281 tgtctttatc tatgatttca tggagggctg ccatgacgtc cccctgggct tcgaccaggg
     2341 tccggccacg cagctgggca ccggcaacgg tacggtggcc gccgccgccg agagcttcca
     2401 tgactaattg tacattgatt tcgcctttcg agcgggcgct gacaccgatg cagtcatcgg
     2461 ccagataata gaaagtgaag ctggcttcga cgctgtcgat gttgatgagc atatccgcta
     2521 cctgggcggc gatgatggtg gcattgcgct gacccttggg gcagctggcc atggcgacgc
     2581 cgtcgctgat ttgggcaccg gacaagatga gggcccggtc cttgacggta tcgaagtcag
     2641 agctgaacag ttcgcggacg atatccggat ccgctccgca gcggcgcaga tacgacgcgg
     2701 cgtcgaaagt gcgcacgccg gtctggacgg cgaagttctt ggtgtcgacg atgatgccgg
     2761 catagagggc cgtcgcttcg atcttcgtca attctacatc ttcctggaaa tattgcaaga
     2821 gctccgtcac cagttcgctc gtcgaagaac tcgacgtttc cgtataggtc agcaagggct
     2881 tcttgatgaa gtccgaactg cggcggtgat ggtcgatgac gacgcgccgg tccgtttcgt
     2941 ccagcaaagc cggggctacg gtcatgtccg ggcggtgcgt atcgacgatg aacagcaggg
     3001 tctggctgtt gcagatgttc tccgccgttt ctgccgaaat gagcaggtcc ttgaaatcgg
     3061 gattatccat gatctgatct tccaggcgcc ggatggcgtc ggtctgattg ctgacgacga
     3121 catgtaccgg cttgcctgcc aggcgggcca tgtgggctac gccgacggca gcgccgatgc
     3181 tgtcatagtc ttcccgttca tgtcccatga ccaggaccat atcgctcgta tcgatgagtt
     3241 cgtggatggc ctgggcgacg acgcgggccc ggacgcgggt attcttttcg acgcagcgcg
     3301 tcttgccgcc gtagaagtga ggcgatccgt cctcttcata gacgacgacc tggtcgccgc
     3361 cgcggcccag agccaggtcg aggccggccc gggctttcgt agccagttcg ttgaaattgg
     3421 ctttgcccgc cgcaatgacg tcgctcggga agagggcgat gcccatgctg agcgtcacgg
     3481 ggatgcggtt gaccgtatgg atggaacgga ttttttccag gaaagaaaag ttatcttcct
     3541 tataatattc cagcgtatcc cggctcatgc aggcaatgta cttctcattg ccgtaagccc
     3601 agataaagca attatggccg gacagctcgc cgataaggca gttgttgacg ttggtcacca
     3661 gcgtagcccg ctggacagcc gtcatcccct tggcaacttc ctccaggttg tcgatctcga
     3721 tatcgcagaa aacggggaca gcagccaggg aattcagctt ctgccgttcc gtatcggtaa
     3781 tgtcttcaaa atagaggatg aggtaattgt catcgtcagc ggcttcggtc tgcagatact
     3841 tgtagacgac gcggtaatag cgttcgccga tgtgttcaaa gaaatatccc gatttccccc
     3901 agaacttatc gactttcaag ttcggcatga tgtggtccag ctgctggtcg ttgtcgatgt
     3961 cgccgaccca gtctcggaag acgctgttgg cccagcacaa gttggactgc atgtcgatga
     4021 tggcaatgcc gataggcaga ttctgcacgg catacgtcga agcctggtcg acgctcttgg
     4081 agatggcatc gaagaactgg ctgatttccc ggttgcgttc gttatggttc ttgcgggtca
     4141 ggacataagc cccgccgatg aggatcaggg ccagcaaggc gatgacccaa ttatatacgg
     4201 caatgatgat aagcagcaag acggtaatca ggaagaaagg ttcttccgac cgccttgaca
     4261 gcaacgtttt aaacatggtt ccgccccctg cgtactaaag cgtacgacct ttttttctat
     4321 aattcgtgag catgtcgaag atgccggcta aaaaggcagc atacgaaaaa acaggcatga
     4381 caaaaaatga gatgaggatc agggcctgaa ggccattccc taaacggaaa cggctgctga
     4441 ggagataaaa gacgaaggca attccctgga tacagatgaa gaagaaggca atctgattga
     4501 gattcatgcc gatgatattg agccaggtaa tatcccgtgt cactccccag tatttcatga
     4561 ccaatgccaa tatatataag tatatcatac tacggggtat ttcccaataa cgaataggca
     4621 aaaacgcctt gagcgaaaag ccgagacggc gcaggatgac ctgggaaatc ttgatgttcg
     4681 cgtaggcgat gatggccgtc cccagacaga ggaacatggg cagcagcgac ggcagcacct
     4741 cccggacctg gttgagctgg gcccgcgttt ctgccaattt gacgtcagac atgccctgct
     4801 gctggtactg ggccatcatg ctgtccatga actgcgtgaa gaaatcgttc atctgggtaa
     4861 agacgttgac gtcgaggaca acataggaca atagggcgat gacgacgaag acggcgacca
     4921 gggccgctgt aaccgtcccc aagaggcgta cagccgacca ttcatagcga aagccagctc
     4981 ccagggccag gccgacggca ccgaagctca ggaattggac caatgccgtc accgggtcga
     5041 tgaacaaccc catgaggaag cctacggtca cgcccagcag caaggtctgg cgcagaccgt
     5101 atttcatgaa aatgatgacc atgggaatgg gcatgatgaa ataaccaata aaagaaaaaa
     5161 tcggcacgta tgtactgaga agcgcaaaga ccagcataat ggcggcaaaa cagccggccg
     5221 tcgtcagcgg ggtgacacgc ctctgctcca taacagttcc ccctttcgtc gggactgaga
     5281 aacagcggag gcacgagatg cctccgctgt cacagtaaca aatatcaaat tatacttcca
     5341 tgatgatcgg caggatcatc ggacgacgcc gtgtcttttc atagagataa cggctcagcg
     5401 tatcgcggac ctgggacttg atgacagccc attcgcggat attaccggct tcgcagcgtt
     5461 cgaggacggc ggcgacgcgg tcatgagctt cgcggagcaa ttcttccgaa tcgcggacat
     5521 agacgaagcc gcgggaaacg atatccgggc cggccagggg atggctcgtc cctttggcca
     5581 gggtcatgac gacgatgacg acaccttcca tagccagctg ctggcggtcg cggatgacga
     5641 tgttgccgac atcgccgacg ccgagaccgt cgatgaagac gcggcctgca ttgacatggc
     5701 ctgtcttatg gccggaacgg ttcgagaatt caaagatctg gccgttgtcg ccgatgagga
     5761 cgtggtcctt ggcaacgccc atcatgacgg ccaggtcgcc gtgcgtcttg agcatgcggt
     5821 attcgccgtg gacagggatg aaatacttcg gtttgatcag ttccagcatg agcttcagtt
     5881 cttcctggct ggcatggccg gatacgtgga ttttcttatc ccggccggcg atgacattgg
     5941 caccgaggcg catgaggtta tcaatggtgc ggctgacacc ggtttcattg cccgggatag
     6001 gcgtagccga aatgatgact gtatcgccag gcatgatgct gacgctgcga tggttgttgg
     6061 aagccatgcg cgacaggccg gccatgggtt cgccctggct gcccgtcgtc aagatgagga
     6121 tctggtcatc gggataacgg ttcagttcat ccggttcgat gaggacacct tcgggaacgt
     6181 cgagatagcc cagttcaatg gcaatctgga cgttgttgac catgctgcgg ccgaggacgg
     6241 tgactttgcg cttgaactgc actgccgtat tgatagcctg ttgtatacgt gaaatattct
     6301 agtcgaatgt cgccaggatg atacggcctt ttgcagcgcg gacagccttc cggaaagcat
     6361 ggacgacagt cgtttccgat tcggtgtagc ccggccgttc ggcattggtg ctgtccgaca
     6421 tgagcagcag gacgccgcgg cggcctaatt cagcaaattt atggatgtcc atctgacggc
     6481 catcgactgg cgtcaggtcc atcttaaagt cgcctgtatg gacaatggtg cctatgggag
     6541 tccggaaata cagggcactg gcatccggga tggagtggtt cgtatggatg aagccgacct
     6601 tcatacagcc gatctgtact tcatcaccat gatgcacttc attcagtgta taattcgtaa
     6661 tgtgattttc tttcagtttc ccttcgatga ggccgcatac gagcttggtg gcgtatacgg
     6721 gcacatttac ttcattgagc aagtaggaca aaccgccgat atggtcttca tgaccatggg
     6781 tgatgacgac ggcccgtact ttgtcccggt tttcaatcaa gtaactcatg tccgggatga
     6841 cgaggtcgat gccgaacatg tcgtcatccg ggaaggccag gccggcgtcg atgacgataa
     6901 tatcattccc gtactggatg accgtcatat tcttgccgat ttcacctaaa ccacctaagg
     6961 gaattaccat gagtttttct tttttagcca aaatatacct cctaaaaata aatatagttg
     7021 tatccgttcc aaccggagcc cggcagacca ggctgcaggt ctatcctata tgtagatata
     7081 acgaacagta atggattttc ttccttctat catattttcc gttatttccg ggactctgtc
     7141 aataatctat cgtctctttt tgtgaaaact cttaactttt cataaaaaat ttacatttat
     7201 tttgccgatt tgatgacttt ttccggcacg gtcagctgtg ttccctgggg cagggccgtg
     7261 caatcgatgg tcaggctgac ttcgctcatt ttgatgaacg actgggctac ggccttgtcg
     7321 gcgtcactgc ggccatattc atcgaccagc gtcagggcca ggctgcgcag ctggtccgtc
     7381 aagggcaggg agatgcggga aatgcgcttc gtcgccgggt cgatggtgac gaccgtcgat
     7441 aagtcgccgc actgctgcaa gccctggagg actttggtca tgacgcggat ctgttcggcc
     7501 gtcatgccgt ttttcttcca ctgggcctgg tcggccggat tatagatatg ggaagcgtcg
     7561 aagacgacgt tgtaatcatt gccgccggca gcggtgactt ttttcacgcc agccaggaca
     7621 ttgtagtcct gacgcaggta atcggccaag ggcttgctgt ccttcaaatc atagctcttc
     7681 ttttcccatt catcgccatc ttcctggtaa tagacgtcga tttttttccc gttctggacg
     7741 gcataggctt tcccttcagg gactttcttc atcgtcgtcg cagcaaaacc cgttacctta
     7801 gcctgggact ggatgacgaa aggccgttcc tggacgtcga tattgttgac gacggcccct
     7861 tcgccgacaa agggcatctt gaggcccagt ttcagcgtat aagagccctg aggatgggcc
     7921 gccatgttgc gggccgcttc ttcatatgtt gcctgcggtg aagcagccag tgccataccg
     7981 ccgatcgatg cagctgccat gaccaggcag cacattttct tccaagaatg tgtcaatctc
     8041 attttgtcca ccctgctttc ttatataatt cataaaagtg atgaatcggg ccatggccct
     8101 tgccaatggg ttcagcatgg gcaatgcctt caaagacgta ctctttcgcc gcaccgactg
     8161 cgtctggcag actcaggcca ttggccaggt tcgaggcgat ggcgctcgat aaggagcagc
     8221 ctgtcccatg ggtactcgtg ctttccaggc gtttcccctt ataataatga aaatcatggc
     8281 cgtcgaagag gatatccgtc gcatcggcaa tgcgatgacc gcctttgacc aggacggctt
     8341 tggcacccat ggcggcaatg gccttggccg cttcttccat gtcgtgcagg ctttcaatgg
     8401 atttccctgt cagcacttcg gcttccggca gattcggcgt caggacatcg gccatgggaa
     8461 ggaggacttc cttcagcgtc tgttcggctt ccggtaccag caggcgatgg ccgctggtag
     8521 caaccatgac cggatcgatg acgatatacg gcggctggta ttgtttcaat ttatcggcga
     8581 tgacgcggat cgtttcaggt acactgacca tgccgatttt gacggcgtcg acgtcgatat
     8641 cttcaaagac cgcatcgatc tgcgaagcaa tgaggtccgg cgtgatgtcc atatagccgc
     8701 gcacgccctg ggtgttctgg gaaacgacgg ccgtaatgac attcaaggca aagcagccgt
     8761 gggcacaaaa agtcttgcaa tccgcagcgg caccggctcc gccagaagga tccgtcccgg
     8821 caatggataa tacatgtttc atcatgagta caacctccaa tttattcttt gtaagaaggt
     8881 cccaaaaatt tcaaggcctt cggatggaca tagaaaagca gcgatgccga accgatgcgg
     8941 ccgccgtgga cgtcgaagtc gaacagggcc atgctgcccg tcttgaagtc gacagccgtc
     9001 cccgtccagg cctgagccca gcggtcaagg aacggcgaat ggccgatgat gcacacgaca
     9061 tcagctgttt cccggctcaa aatctcgttg tatacggcga ctaagtcacc agaagcgatg
     9121 gctttatgcg tatggaactg ctcaataggc agatgctttt ctaaataagc ggctgtctga
     9181 cacgtccgca cgtatgggct gacccacagg gccgttttcc ccgacggcca ccatgacgag
     9241 gcccatttcg ccatggacga agcctgatta cacccttttt cagtcagttc acgcagttta
     9301 tccggctgat gcagatcaaa aggctgggcc tttccgtgac gcattaaaca aacatacata
     9361 aactactccc cttttatgta ccccttttat tcggcaacat gctccaggcc gacgtcgagc
     9421 agcgtaaaga catgggcatc ggccagcgaa tagtatacgg tcttgccgtc ccggcggaac
     9481 tggaccagcc gggcatcgcg caggatgcgc agctgatggg acacagcaga ctggcccata
     9541 ccgagttcat cggcaatatc ccggacgcag acttcctgat tcatgaggac gcgcaatatg
     9601 cgcagccgtg tcggatcgcc gaggactttg aagacatcgg ccaaaggctg gatatattgt
     9661 tgtgaaagtt ccggtgctcg tcgtttcaat gcaatcgctc ctaatgctgt ttatcactgt
     9721 ctgtcttaat cgttttatta tagcacactt ttcacatata ctgctagagg cgtcacaaat
     9781 aaggcccgca cagggtacag acaaggtaaa tgaattatga aggaattata aaaagtccta
     9841 aaaaggcgtg aaatgtcctg gcaaaagcct ttcctctaaa tcaaaaatgc cctataatga
     9901 taacaaaatc gcaacaaaat acttttactt aggagtgaat gtcatgaatg aaaccatcca
     9961 taccctcatc acgcggcgca gcgtccgcaa gtacaaaaag gcccagatca gcgatgaaga
    10021 cttgtatctg attctccagg ccggcaccta tgcagccagc ggtcgtggcc tgcagccctg
    10081 taccatcgtc gtcatccagg atacggatac cctggcccgc ctgagcgctc tcaacgccaa
    10141 aatcatgggc acgacgaatg accccatgta cggcgccccg acggtcctcc tggtcctggc
    10201 cgacatggaa aaatccccca acgcctggtc cgacggctgc ctcatcctgg gcaatctcat
    10261 gaatgccgcc gcttccctgc acctcggctc gtgctggatc aaccgggcca aagaagaatt
    10321 tgaaacgccc gaagggaaag ccctcctcaa agaatggggc ctgccggaac acctcgtcgg
    10381 cgtcggccac tgcatcgtcg gctacggcga cggcccggaa ccggccgcag ccccgcggaa
    10441 accggatttc attaaatttg ttaagtaaat agtggttagt ggctcgtagg ggcgccccgt
    10501 ggggcgcccg cagaccgcag ttcgcaggcc gcatggctct cctcatagga gagctgtcag
    10561 caaagctgac tgagaggttg ctttttcttt tttataaatt taataacaac cactactaga
    10621 aaagggacta tcgtatgaag gcttatacca tcatgcgtag tcccttttct agtaaggccc
    10681 gccacgtggg cgacccgtta ggaatttggg tgatgctcct agcaaatgac cactaaccac
    10741 taaccactaa ccactaacca ctaaccacta accactaacc actaaccact agcgattatc
    10801 tccccttctt acattccggg caatagccgt agagttccag gctgtggccg acgatgtcga
    10861 actgtgtctt ttgggccact tctttttcat agtcgctgag cggacaatga gctagatcca
    10921 cgactttatg gcattgtaaa cagatgagcc tgtgtgtatg gccgggacgg cggagggaaa
    10981 agccgtattt cccttcatcg gggaggaata tcttttctgt cagtttacgc tctgtaaaca
    11041 tttccaggat gcggtacact gtggaaaaat tgactttaca tccttgttcc aacagggctg
    11101 catagacggt ttcgacggtc acgacactgg tttcccggct caggatatcc aggacgccgt
    11161 tgcgggcctt ggtactcttc aggccatgtt cgcgtaaaca ggctttataa tcgtcattca
    11221 tggacggcgc ctcttttctt gcagggaaaa gacacattct ttataggcaa tgaccagaag
    11281 cagcatggca actgacgtca aggccgtgac gccgccaggc gcagcatcga tgtagtacga
    11341 cagcagcagg cccgacagaa tgtcgatgaa gctgaagacg acggagtaaa tgagggtccg
    11401 ccggaagcct ttgcgcagct gcagggccgt cgccacgggc agggcgatga gggagctgat
    11461 gaccaggatg ccgacgatgc ggatagaaac ggaaatcgtc gccgctacca ggacggcaaa
    11521 gatatagttg atgagcttgc tgcggacacc ggcaatctgc gctccgtctt catcaaaggt
    11581 cagcatgacc agctggggat agagtctggc aatgacggcc agggagatga cgctgagggc
    11641 gacgacggag tagacatcgc ccgtcgacac ggtgaggata ctgccgaaca agaaggattc
    11701 gacattcgtg tggaccaggc cggcactgat gatggtaatg gcaatgccca cggagagaga
    11761 taagatgatg accaggatga gctcggcata acggcggaag cggcggcgca ggacttcgat
    11821 gaggatgccg aagaaactgg tcaggccaaa ggcgctgaga acggggttcc agtcaaagag
    11881 gagacccaca gccacgccgg ccagggacgc atgggacaag gcgtcgccaa tcatggaata
    11941 gcggcgcagg accaggaaca tgccgatgag cgggcagacg atagaaatga gcagggctac
    12001 gataaaagca ttctgcataa aggcatattc aaacattact tatcccctcc catcgtggct
    12061 gatacatatt catcgagata tgccttgggc gagcagaaat ggccgcggcc accgacgacg
    12121 tggaacatct tcgtcgaatt gcggacagcg aatttcagat tgtgttcgac cgtaatgacc
    12181 gtgacgcctt tattttcatt taaatccttg ataaacgggt agagctcttc ctgactgcgc
    12241 acatcgattc ccgtcgacgg ttcatcgaag atgagcaggt ccggatggcc catgagggcc
    12301 cgggcgatga ggaccttctg gcactggccg ccggacaggt tgccgatgag ctggtccttc
    12361 agggaataga cattcatctg ttccaggcag tcccggatga tttctttatg cttcaagtgc
    12421 aggacctggc ggtaacagtt gaggacttcc cagaccgtaa tcgggaactg gctgttcagc
    12481 gtctcgaagc gctggggcac ataggcccga tggcgatagg tattggaaat ggacccccag
    12541 ctcggcgaca acaggcccag gatgagtttg atgagcgtac tcttgccgct gccgttttca
    12601 ccgacgacgg aaatatagtc gccgtcgcgg atggtcatgt tgacatcttc taacaggtac
    12661 tgaccgtgtt cataggcgaa ataaacgtgc tgtaaatcaa tcatagcttg cctcccttgt
    12721 cgtagtctcg agcccttatt atagccccgt gaaaacgaaa atgcaaggca tttgcaaata
    12781 attgacaaaa aaaggcgccc gtgatacgat ggacgccata ggaggtctta tcaatgttgt
    12841 tacataccct caaccgcctg gcccgttacg gcatggtcct ggcggcctgc ttatgcatca
    12901 tcggcctggc tggctgtggt cccgccgccg atgtccccga tgacgggaaa ttaaaagtcg
    12961 ctgtcacttt cgacgctctg aaagagttca ctcaagccgt aggcggtgat aaagtcgatg
    13021 tttacaccct catccccgat gggacagagc cccatgaatt ccaaccgacg acgcagacta
    13081 ttaaacatct cggtaaatct cagctcttcg tttacagcgg cctgggcatg gaaccgtggg
    13141 cccctaaggt cgtcgaagca gccaagaata catccctcca tacggtcaat gcctcgcaag
    13201 gcgtcacgcc catcgccctg accgatgagg aagatgtcaa agaacacggc gcctacgacc
    13261 ctcacatctg gctgagcctg gtaaacgccc aaatcgaagt atctaacatc gccgccgccc
    13321 tggctgaagc cgacccggcc aacgccgact attaccgcaa caatgcggcg aattacaaaa
    13381 aacagctcca ggccctgcaa gacgaatatg cccgcaaatt cgccagcgtt ccccgcaagg
    13441 atttcgtcac cggccacgct gcctttgcct acctctgccg cgacttcggc ctgacccagc
    13501 acagcgtcga atccgtcttt tccagcggcg aacccagtgc ccgccagctg gcccgactgg
    13561 ccgactattg ccgggccaac gacgtgcgga ccatcttcgt cgaagacatg gtcagcccga
    13621 aaacgtcaga aaccctggcc cgtgaagtcg gtgcccgcgt cgaaacgatc cataccatcg
    13681 aaacgagtga agatgacgaa tcctacctgg atcgcatgcg cgacaacctg gaaaagattt
    13741 atgccagcag cctgcgctaa aatggcaaaa aataaagtaa ccatggagaa gaatatgaaa
    13801 cataaaacat ataaagccgc aatttttgat atggacggta ccgtcctcaa taccctggaa
    13861 gacctgacca gcgccttgaa ccacgtctta gccgaaacag gtcacgacca tgcctatggg
    13921 acagcccagg tccgccaatt cttcggcagc ggcgtccaag tggccattac ccgcgccttg
    13981 gccattgaaa aggggattgc ctcttacgaa cagctggaat acgtcggcca ggacggcgat
    14041 acgattacac cgcgtgtcga tgcccaggaa gtccgccgca tccaggacat cttccgtccc
    14101 tactatgccg cccactgcgc catccagacg ggagaataca ccggcatcag ccagctcctc
    14161 cgccagctcc gggccaatgg catccaaacg gccgtcgtct ctaataaacc cgacccggcc
    14221 gtacagaaac tgacagccga ctattttcag gacctcttcg atctctccct gggcgaacag
    14281 ccccagatac ggcgaaagcc tgcgccggac atggtcctca aagccttgaa agacttagga
    14341 ctcacgccgg aacaggctgt ttacatcggc gattctgaaa tcgacatcca gaccgccaaa
    14401 aacgcaggcc tggactgcat cagcgtcacc tggggttttc gttccaaaac ttttttgaaa
    14461 gcccacggtg cccgcttcct cgtcgatacg ccggaagaaa tcgccaggtt cttttaaaaa
    14521 actagcgatt attctccctc aggtacgaac caaaggccat tattgttttt cttcatatac
    14581 tctttgaatt cgtcggaatg gtatgcatcg gcaatatctt tgacccattg tgcatcttta
    14641 tctttatcct ggacaacgag ctgtaacagg agatgcggta agatatcttc tttagccaaa
    14701 tccgtctgag gatcgatttt agcattgtaa atgatagcgc cagtgatgac aatgtaactg
    14761 aaatcactgc gtacagatgg gatagttaaa gatttcattt ctgtaaattt caagtgatgc
    14821 ggattttcta cgatatcctg ctgggtaact gtcgacaagt ctttattggg atccaattta
    14881 atccagccga ttttctggag cagggcataa gcacgagctg tattagaagc atcgtttgga
    14941 acggcaatcg tatcgccatc agcaacctgg tccaaagaag tcttagaacc cgggaaaata
    15001 ccggccggaa cagtcggaat cggcgtaata gccgctaaat ggccattttg tttttgatta
    15061 aagttttcca tataagccgt atgctgttca acgttgaaat ctacttcacc ttcattcaaa
    15121 gctacatcgg cctgcaataa atcagacata tccttaccgg taactttata gcctttcttt
    15181 tccaaaatag gcttaattcc cttttcaaag aggtcggaat acggtccctg agacttactg
    15241 tacgtcagtt cctttttgtt ggcgctagat gcattattgc tgtcgctgcc acaaccggca
    15301 agaaaagcag cgcccatgat gacggccatc gcaatgacac caaatttttt ccatgttttc
    15361 catttcatat gaataccccc taaatgaaca tccctatata tgataagtgt gcaaccttaa
    15421 tgattgcgcg tcttataggc aaaatgattg ccaatggatt gaatgatttg tacaaagata
    15481 actaagatga cgaccgtaaa gaacatgagc ggcgtattca atcgttcata gccataggtc
    15541 aaagctaagt caccgacgcc accggcccca atgacaccag ccatagctgt cgctccgata
    15601 aggccgatag tcccagtcgt aatcgataga ataagagagc ttttagcttc aggaagcaga
    15661 aaataccaaa cgatttcaaa ataagacgct cccatggact gcgccgcttc cacgatcccc
    15721 ttgctgactt ccaaaatgga attttcaaaa agccgggtca agtaaggcgc aatgaaaatg
    15781 accagtggaa ctaaagccgc tgtcgtccca atacgagtcc caacaatcat tttagtcaat
    15841 ggcaaaataa aaaccatcaa gatgataaac gggatactgc gaataatgtt gataactgta
    15901 ttgagaacag tatagacata tttattcttc aaaatgccat catcattagt cagcaccagg
    15961 acaacagaca tgataattgc caaaatagtc cctaagaata acgaaacgaa gaccatatat
    16021 acagtctgtt ctgccgccaa aataatctgc gacataggca cacctaaatc aatcattaca
    16081 aatcaacctc ctgccactga acctgctgtt catcaatgta ttgaataacc ttggcaatct
    16141 ctgcatcatt cccaataatc tgaaggatga agataccaag aatcgtcttt tgtaattcat
    16201 tgactgatgc aaagaggata ttcgtctgga cggaaaaggt tttgttgata tgatacataa
    16261 ggtggtctgt cgcattatcg ccaaggaaac gaagtttcaa aattttataa ttatcttttt
    16321 cttcatgaat cttggcaacg acagacggcg gaatctggtc gggaataacc gtacggacaa
    16381 attttttagt catatcctgc tgaggattac tgaagacttc cagcacagat cctctttcta
    16441 cgactttacc atgttccatg acggccacac gattgcatag acgctgaata acttgtattt
    16501 catgcgtaac gataagcatg gtaatattta actctttatg aatccgttca aggagctgca
    16561 aaatcgcttc cgtcgtttcc ggatccagtg cacttgtcgc ttcatcgcat aagagaatag
    16621 acggattcgt agccagtgcg cgagcaatgc caacacgctg cttttgcccg ccagaaagtt
    16681 gacccggata agcatcggcc ttatccgcca gattgacgta ttccaaaagt tccatgacct
    16741 ttttatgcat cttgtcttta tccgctttat taagccgtaa aggaatagct tcattgtcaa
    16801 aaacggtcct cgagtcgaga agattgaatt gctgaaagac cataccaatg gatttgcgaa
    16861 gtttacgcat ttcattgtga gaaattttac tcaaatcttg gccgttgatt tctacatgac
    16921 cttcggtcgg catttctagg ccgttgacca tgcgcagcag cgtcgattta cctgcaccgc
    16981 tgaacccgat gacgccaaac atatctccgt catcaatagt cagtgataca tgctgtaatg
    17041 cttgtgtcgt aacacctttg cttttaaagg tcttgctgac atctttaaat tgaatcatcc
    17101 cttgctgctc ccttcttgct gaatctgttc tactgcctgg caggccagtt cagccatata
    17161 ttgtgaagcc ggatatagtg cggctggatt taccttgaat ttagcattgt gattcgggta
    17221 ggaatggcca gtcccgacaa aaacaaaata gccaggtacc ttatcgtaat aaaaggcaaa
    17281 gtcttcgcct cccagtgacg gggccatcgg ttgtttatca aagccctgtt tatcagctac
    17341 cttacgggct aagattcccc aatgagcatc attaatcaat gccggcggcc ctggaatcca
    17401 atggacttcg gccttggcgc cataagcagc agcggtggaa gaggccaagg attcgatatg
    17461 attgcgaata gcctgccgcc cttctttact catactccgc gtcgtacctt caacaaaagt
    17521 ttcatcagga atgacattcc agttgtgacc gccttcaata tgggtaacgc tgaccaaagt
    17581 agcggcgaag ggatccatat tcctgctgac cacagtctgg acagcaccga caaaagcaga
    17641 cgctgttacg atgggatcga tacctttatc cggatgagct gcatggacgc ccttgccatg
    17701 aaaagtaata cgaaaagcat cgacagccgc catggccgga ccatcgatga tccccatttg
    17761 accgacatcg agaaggggcg tgcagtgcat tccaaaaatc gcatcgacat catcaataat
    17821 gcccgtttca atgattttca aggctccgct cggaccttct tctcccggct ggaaaataat
    17881 acggatagtt ccttgaatat cggcttcccg tgctttcaat aaataagctg cccccaaaat
    17941 agaagctgta tggaagtcat gtccgcaagc atgcatacaa tcgggatgtt ctgaccgatg
    18001 aggtacgtca gctttttccg taaccggcag ggcatcaata tcgcagcgga tagcaacaat
    18061 aggatgtttt cctgttccga cctctgcaac gacgcccgtc tccaaaggca gattcaaaat
    18121 atgaagcccg cctttttgta agtcttgacg gatccgtttg gtcgtctcta tttctttaaa
    18181 agatagttcc ggatgacgat ggaaccattg aaattcatca tgaatatatt gatttatgtc
    18241 ctgtatcatt cataacccct ctttctcatt ttctctatat ccttctgtgg atatacaaac
    18301 aggccaccat ctcctatgac agagacggtg gcccgcggtt ccactctgtt tacagccaaa
    18361 actgtatctc ataaccgtgt aacgccggtc agacgcagct gcatactaaa ttccgcagcc
    18421 atgcaaccaa aggcattcca tcctgtttcc caacgatgca ggctttcagc cccagacctg
    18481 cactctctga cgcagtacac agatgtactt gttttggtta tcgcatttct caatgtttac
    18541 gcctatggta ctattcaaaa ggctacttgt caagtatagt ttcatctttt tatatatttt
    18601 ctaataaatt agcaaaaaaa ttcacgttag catatcatta taacaaacat tattttgact
    18661 cgctcagata ctgcttttaa agcgaaaaat cgatgacttc ctcattcagt ggaaagagcg
    18721 tcctcagaaa aagccgtttt tcgtcgaaaa gcccgtctta tagtgacatc cgcttcggca
    18781 tcaagctcat cggcggcaac atcggccatg aaaacgatat ttacaccttt ccttatttct
    18841 gcacgttctt agtccgcgat tttctccgcc agctcccacc ggctccggct catatggtat
    18901 aataggagca gaatgattac gtttgtgagg tgcggatatg tcgacggaaa aacggatttt
    18961 ttactcgatt tgcctgatca gttttgtcgg gccttttctt tctacgtcta tcaatatcgc
    19021 catccccgtc atggccggac agttcggcgt cgcgcgggcc cgcatgagcg gggtcgtgac
    19081 gatnnnnnnn nnncccagac cctgacgatg ctcatcatct tgcgggccat ccagggcatc
    19141 gccctgtccg gcgtctacgt atcttacatg cccctgctcc tggccacgac ggatgaagcc
    19201 catcagggcc atatcctcgg ccgggccgtt gccctgacct atcttggcct gtccctgggt
    19261 ccggtcatcg gcggcgccct gacgcagttc gtcggctggc gctgtatctt cctcttgtct
    19321 gccctggcca tcatcatttc gtacgccttc atccggccca tcaaagaaga atggtatgcc
    19381 aacggcgctc ccttcgtcaa cctggtcagt acagccttat cggtcagcgg catcctctgc
    19441 gcgctgtacg ggctatcatc ctttgaagat tacagtgctt ttttctggat tggcctggtc
    19501 ttgctggtca tcttcgtcat ccatgaaggc aagtccttcc accccctgct gccactctat
    19561 ctgttccgca acctgacttt ttccatgtcc aacctggctg ccctcatcca gtacagctcc
    19621 acgtatgccg tgtccttcct gttatccctc tatttccagg tcatcctcgg cctgccgccg
    19681 gctgtatccg gagccattct cctggtccag cccatcatca tggccttctt gtcgccccgg
    19741 gccggcgact tgtcggataa atacggccca cggggcattg cctctatcgg cctcgtcctg
    19801 acggccctgg ggctgacggc attcgccctg ttcccgcaca tccccgtcag cggcgccgcc
    19861 atattcctgg tcttcatcgg cctgggcgca gccctgttcg gcgcgcccaa caacagcgcc
    19921 atcatggggt cggtcaaaaa gatgcatcac ggcatcgctt cgagcatcct ggccttatcg
    19981 cgcaacctcg gccaggccct gagcatggcc gtcgtcacct tcatcatgac gacagagacg
    20041 gccaaacagc cgtcttatgc ggatggcgtc gtagccgccc tgcacgcttc gttcgtcatc
    20101 ttggccgtcc tctgtttcct cgccgtcgcg gcctcgctgg cccgcggcac aagtcccaaa
    20161 aaagtgtaaa ttaggaggta tttccatgaa aaaacaactc atcgcagccg ttacggttgc
    20221 atcgctcctc ggctttgccg gtggcacggc cgtccaggcc ttcaacctcg gctctatcct
    20281 gaaagtcggc ggcatttccg tcctcgtttc caaatacggc gattccatca atgatttcct
    20341 caacaaactg ctcatgaaga acggcgtcgg cacggactac gcgacgaaag tcgtccccat
    20401 cgtcagtgtc ggtaccggta aagatctcgc cgccctccag gtcat
//
LOCUS       DAWCYS010000009        17192 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_211_length_17192_cov_7.281905, whole genome shotgun sequence.
ACCESSION   DAWCYS010000009 DAWCYS010000000
VERSION     DAWCYS010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17192)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_17192_cov_7.281905"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4714 a         4480 c         4338 g         3660 t
ORIGIN      
        1 acggcaacaa acgtgtcgtt tcgcgcgtca tgcgcgagga agagatgcct tttctgcccg
       61 atggcacgcc tgtccagatc gtactgaacc ccttgtgcgg accgtctcgt atgaatatcg
      121 gtcagatttt ggaaacccac cttggctggg ctgtccacga actgggcaaa cagatcaagg
      181 aaatggatcc gggcctggaa gaaaaactcc gcgctgccgg ctatgatttc gataaatacg
      241 gcatgcccaa accggacgtc gccggtgtcc atatcgcaac accggtcttc gacggcgctc
      301 aggccgaaga cgtctttgaa gccctgaaga tcgccggcct gccggaagac ggcaaatccg
      361 tcctctatga tggccgtaca ggggaaccga tggaccgccg cgtcactgtc ggttacacgt
      421 acttcctcaa actgcatcac ctcgtcgacg acaagatcca tgctcgttcg acaggcccgt
      481 actccctggt cacgcagcag ccgctcggtg gtaaagccca gttcggcggc cagcgtttcg
      541 gcgaaatgga agtctgggct ctggaagcgt atggcgcagc ctataccctc caggaaatgc
      601 tgactgttaa gtctgacgat gtcgtcggcc gtgtcaaggc ttacgaaaag atcgtcaaag
      661 gtgaaaatat tcctgaaccg ggcgtaccgg aatcgttcaa ggtcctcctc aaagaactcc
      721 aggccatcgg cctcgacgtc cagatcctga acgaagacgg cgaagaagtc gtcatcaagg
      781 aactcgacga tgaaacggaa ccggaacaga ctgatgaact ccgccgcgtc atggaaggtg
      841 aaaatggtcc ggaaacaccg gctgcaccgg ctgaagacac tcctgccgaa ggagaagaag
      901 gcgaagaaca ggaacccgtc gccaacggcg gcgaagatga catcatgagt gccatgagcc
      961 tcgatgccgt cgccgaagct gaacaggctg acgatgatga ataacgagtc gatagaaggg
     1021 agcggtatta gtgcttgatg tgaatgaatt cgattccatg cggatcggtc ttgcttcacc
     1081 ggaaaagatc ctcgaatggt cccacgggga agtcaagaaa ccagaaacga tcaattaccg
     1141 tacattgaaa ccggaacggg atgggttgtt ctgcgaacgc atttttgggc cgacaaagga
     1201 ctgggaatgt cactgcggca aatacaaacg tatccggtat aaaggtgtcg tctgcgaccg
     1261 ctgcggcgtc gaagtcacgc gcgccaaagt ccgtcgtgaa cggatgggcc atattaaact
     1321 ggctacaccg gtatctcata tttggtattt taaaggcatc cccagccgga tgggcctgat
     1381 cctcgatatt tctccccgtt cgctggaaaa agtcttatac ttcgcctcgt acatcgtcct
     1441 cgatccgggc gatacgccgc ttaccaagca gcagctgatg acggaaacgg aatatcgtca
     1501 gtatctggat atgtacggcg ataaattccg cgtcggcatg ggcgctgcag ctgtcaagga
     1561 actgctgcag ggcctggacc tggaccagct ggacaaacag ctccgcgatg aactccgcga
     1621 ttcgtcgggc cagcgccgcg tccgcgctat ccgccggctg gaagtcgtcg aagccttccg
     1681 ccactccggc aacaaaccgg aatggatgat tatggatgtc atcccggtca tcccgccgga
     1741 actgcgcccg atggtacagc tcgacggcgg ccgcttcgct acgtctgacc tgaacgatct
     1801 ctatcgccgg gtcatcaacc gtaacaaccg cctgagccgc ctcttggaac tgggcgcacc
     1861 ggacatcatc gtccgcaacg aaaaacgtat gctccaggaa gccgtcgatg ccctcatcga
     1921 taacggacgc cgcggccgtc cggtcacggg cccgggcaac cgtcccctga aatccctgtc
     1981 ggatatgctg aaagggaaac agggccgttt ccgccagaac ttgctcggta aacgtgtcga
     2041 ctactctggc cgttcggtta tcgtcgtcgg tccggaaatg aagatgcacc agtgcggcct
     2101 gccgaaagaa atggccctgg aactcttcaa gcctttcgtc atgaagaaac ttgtcgaaaa
     2161 aggcattgct accaatatca agaacgctaa gaagaaagtc gaacgcgtca acagtgaagt
     2221 ctgggacgtc ctggaagacg tcatcaagga acatcctgtc ctcttgaacc gcgccccgac
     2281 actgcatcgt ctgggtatcc aggctttcga accgatcctc tgggaaggcc gcgccatcaa
     2341 actgcatccg ctggtttgca cggcattcaa cgccgacttt gacggtgacc agatggcctg
     2401 ccatctgcct ctttccgtag aagcccagtc cgaagcgcgg acgctcatgc tttcgtctca
     2461 taacatcctg gctccgaaag atggcaaacc ggtcgccgtc ccgacacagg acatggtcct
     2521 cggtgcttat tacctgaccc tggtcaaacc ggctgccaag ggcgaaggca agattttctc
     2581 caactacgat gaagtcatgc tcgcttacga tgccaaagtc atcgacatcg aagccctgat
     2641 caaggtccgc atccctggcc acggcctcat cgaaacgacg gcaggcaaac tgctctacaa
     2701 ctatcagatc tatgaaccac tgcgcctctt ccatacggat aaagtcatgg tcttcacgga
     2761 cccgaaggac acggtcttcg cctatgaaaa cggcctcatc gactggtccc acttcgtcaa
     2821 gatcaaagac gaagaaggcc atatccgcga ctatggtttc tcgcagctga aagaaaaatt
     2881 cggtgtcgac ctcacggcta cgcgcccctt gccgaagcgt aagatcgacg aagccgctgt
     2941 ggctgaattc aagaaagata aagaatatgt cgacgtcaac tgcctgggcg tcctcatgag
     3001 caagaaacag atcggcaaac tcgtcgatgc ctgcttccac ctcgtcggca ataccaagac
     3061 ggcagaactt ctggacaaga tcaaggccat cggctaccat tacgcccgcc aggccggtat
     3121 gtccatcgct atttctgata ttcagatccc ggcagaaaaa tgggatatcc tcaatgaagc
     3181 cgacaagaag gtccagaacg cctatgccat gtatcagcgc ggcttgatta ccgaaaacga
     3241 acgctataac aagagcgtcg aaatctggac aaaggccact aacgacgtta cggatgtcat
     3301 gatggataac atggacccgt tcaactcgat cttcatgatg gctgactccg gcgctcgagg
     3361 cagccgtgac cagattcgtc aggttgccgg tatgcgtggc ttgatggctg acccgtcggg
     3421 ccgtatcatc gacttgccga tcaaagcgaa cttccgtgaa ggcctgtccg tactggacta
     3481 cttcacctcg tctcacggcg cccgtaaagg cttggccgat acggcactgc gtacagctga
     3541 ctcgggttat ctgacccgtc gtctcgtcga cgtctctcag gacgtcatcg tccgtgaaga
     3601 tgactgcgac gtattcggca tcgacctcgt ccgcgaacgg gcccgtctgg ccagctcgtg
     3661 ccgccaggct gtcaacctcc tgcgcgacaa gctcatcggc cgcgtcctgg cccgtgatgt
     3721 agcggatccg gaaaccggcg acatcgtctt cccggcagaa catctcctga ccaacgacga
     3781 tatggatacg gtcatcgaac accagatccc gaaactctcc ctccgcggca gccagatgga
     3841 tatcaacgat gacctgaacc acaccaacgt catcgaaacg gtttccctcg gtgctccgga
     3901 agaaggtttc cgcgcagcta tccgcaaatc catggcggaa aatatgctcg gcaagactgt
     3961 cgaaaaagcc gtcatcgatt ccaacggcat tgaaatctgc gccaaaggca cgccgctcac
     4021 agaagatgcc atcgaccgca tcctcaacag cgacgtcgat gaagtcaagg tccgcaacaa
     4081 cgacatccgc ggcgtcgaag tcacggctat cgtcgaagac agcggcaacg gcgtcatcga
     4141 atcgctggaa gaccgcatcg aaggccgtac ggctgccgaa accctggtca acccggaaac
     4201 gggcgaagtc atcgttgccc tcaacgaaga aatcatggca gaccaggcca aagaaattgc
     4261 aaaatactat gataaagtcc ggatccgcag cgtcctcacc tgccgcagcc gttatggcgt
     4321 ctgcgcgaag tgctatggcc gcgacctcgg tacgggcggc aaggtcaatg tcggcgaatc
     4381 ggtcggcacc atcgctgctc agtccatcgg tgaaccgggc acacagctga ccatgcgtac
     4441 cttccatacg ggcggtgtcg caacggccgg cgatatcacc cagggtcttc ctcgtgtcga
     4501 agaattgttt gaagcccgta agccgaaggg caatgctatc gtcacggaaa tcagcggtac
     4561 cgtatcgatt accgaaaaag aagaccatca cgatatcaac atcgtccatg tcacttccaa
     4621 ggacggtgaa gaacggagct acaccatccc gttcggttcg cacctcgtcg tacatgaagg
     4681 cgacttcatc gaaaagggca gccgcatcac ggccggctcc atcaaccccc acgacatcct
     4741 gcacatcctg ggtgtcgaag ctacccagcg gtacctcgta tacgaagtac agaaggtata
     4801 caaatcccag ggtgttggta tcaacgataa gcacatcgaa gtcatcgtac gccagatgct
     4861 gcgcaagatg aagatcgaag acgccggcga tgccgatgta ttgccgggcg attatatcga
     4921 cgtcaacatc tttgaagata tgaacaagcg catcgaaagc aatggcggca agcccgctat
     4981 cggccaaccg atgctcctcg gtatcaccaa ggccgcactg gcaacggact ccttcctgtc
     5041 ggctgcatcc ttccaggaaa cgacccgtgt cctgaccgat gctgctatca aaggcaagat
     5101 cgacccgctc ctgggcctca aagaaaacgt catcatcggt aaactcatcc cggctggtac
     5161 gggcatgagc cgctaccgca agatcaaggt cgtaaagacc gttccgtcta tcagctatga
     5221 agacgaagaa ggcaaccccg tagacgcacc ggaaggtgcc gatacgacac cggaagccgg
     5281 acattaagag agtaaaaaag ggctgtcgca tgtgcgacag cccttttttg ttgttagtgg
     5341 ttcgtcgttc gtaggggctc gcacgtggcg agcccgtttt gtatatatgg aaaggctcgt
     5401 ggctcacgaa tcactaggac acgtgtaata aaaaatgtac tgattacgca gtttttacca
     5461 aaaaactcat tgacatataa taagcgtgat gataaaatat ctaagtgtcg caatgaaagt
     5521 attgcgtcta aattgcaaga cgtaaggagt gaggtttcgt gagcttggaa ctagctgcca
     5581 gtgtccgcaa gaccgtgggc gcgaagcaaa ccctgaaagc aatgaaacga aacgaagtca
     5641 cacaactgta catcgccaat gattgcgatg aacaggtcgt agcgccgctt attgcagaag
     5701 ccgaaggcgc tcacatccct gtcgaccgcg cgtataccat gacggagctg ggcatggcct
     5761 gccacatcaa ggtaaaagct gcagccgtcg gcttgttaaa ataattttcg gtaacatcat
     5821 ctcatgagct ctgctcgttg atgattacat tataaatcta attgtttgga gaggaggtgc
     5881 ccaaatgcca acaatcagcc aattggttcg taaaggacgt caggttgccg tcacaaaatc
     5941 gacagcaccg gctttgaaaa actgcccgca gaaacgcggt gtttgcactc gtgtgtacac
     6001 agctaccccg aaaaaaccta actctgctct gcgtaaagtt gcccgtgttc gcttgacgaa
     6061 ctccatcgaa gtaactgctt atattcccgg cattggtcac aatttacagg aacacagtgt
     6121 tgtattaatc agaggcggtc gtgtcaaaga ccttcctggt gtacgttatc acatcgtccg
     6181 cggtgccttg gatacagctg gcgtagcaga tcgtcagcag tcccgttcta aatacggcgc
     6241 taagaaaggc aaataagatt tgccttttaa gatgaacaag atgacttaca tagcatagag
     6301 gaggaattag acatgccaag aaaaggcccc gtgccgaagc gtgatgtgct gccggatccg
     6361 gtgtaccatt ccaaattggt aacgcgcttc atcaacaaag ttatgttgga cggcaaaaaa
     6421 ggtgtcgctg aaaccatcgt ttatgatgct ttcgacatca tccgttctaa aatgaataaa
     6481 gatcctttgg aagtattcga acaggctctc aacaacgtta tgcctgtcct ggaagtccgt
     6541 gcccgccgcg tcggtggtgc taactaccag gttcctgtcg aagtacgtgc agaccgtcgc
     6601 ctgtccctcg gtatccgctg gactgtcggc tatgcccgta aacgcggcga acgcacgatg
     6661 aaagaaaagc tcgctggcga attgatggac gccttcaaca acacaggcgc tgctatcaag
     6721 aaaaaggaag atactcataa aatggctgaa gctaacaaag ctttcgcaca ttatcgttgg
     6781 taatttacga tatttattat tttttgagga gtggaaaaaa ccgtgccaag agaattttct
     6841 ttggaaaaaa cgagaaatat tggcatcatg gctcacatcg atgctggtaa aaccacgaca
     6901 acagaacgta tcctgttcta caccggccgt gtccacaaga tcggcgaagt tcatgacggc
     6961 gctgctacca tggactggat ggctcaggaa caggaacgtg gtatcaccat cacgtcggct
     7021 gctacgacag ctcactggaa gggataccgc atcaacatca tcgatactcc ggggcacgtc
     7081 gacttcaccg ttgaagttga acgctcgctg cgcgtactcg acggctctgt tgctgttttc
     7141 tccgcaaagg gcggcgtaga accgcagtcg gaaacggtat ggcgtcaggc tgaacattac
     7201 catgttcccc gtatcgcatt cgtcaacaag atggatacga ccggtgctga cttcctgaac
     7261 gttgtcgata tgatgaaaga tcgtctccag gcgaatgccg ttgctattca gctcccgatt
     7321 ggcgctgaaa caaccttccg cggcatcatc gacctcatta ccatgaaagc agaagtctat
     7381 gacgatgcac tcggtaaaga aatcgaagtt gtcgacatcc cggaagacga attagaagaa
     7441 gctaagaaat atcatgacat catggttgaa gctgtttgcg ataccgatga agacctcatg
     7501 atgaaatacc tcgaaggtga aaaaatcacc atcgacgaat tgaaagcagc tatccgtaaa
     7561 ggcgtctgca cgaacaaatt gttcccggtc ctctgcggtt ctgcttacaa gaacaaaggg
     7621 atccagatgc tcctcgacgc tgtcgtcgac tacatgccga gcccgttgga tattcctccg
     7681 gtcaaaggca ccaaccccga cactgaagaa gaagaaaccc gtgaagctga tgacaatgca
     7741 ccgctttccg cattggcatt caagatcatg gctgacccct tcgtcggcaa actggcattc
     7801 ttccgcgttt actccggtac gttacaggcc ggtacctatg tctacaactc gacaaaaggt
     7861 aaaaaagaac gtgttggccg tatcctccgg atgcacgcta accaccgtga agaagtccgg
     7921 gaagcctaca cgggcgatat cggcggcatc gtcggcctga aagatacgac tacaggcgat
     7981 acactgtgcg atgaaaaaca cccgatcatc cttgaaaaga tggaattccc ggatccggtt
     8041 atttccgtag ccgttgaacc gaagacgaaa gctgaccagg aaaaaatggg cattgccttg
     8101 gctcgtctgg ctgaagaaga cccgacattc aaagtcaaga ccgatgctga aaccggccag
     8161 accatcatct ccggtatggg cgaactccat ttggatatca tcgtcgaccg catgtcccgt
     8221 gaattcaaag tagactgcaa cgtcggcaaa ccgcaggttg cttaccgcga aaccatccgc
     8281 aaagatgtca agtctcgtgg tttcttcaag cgtcagtccg gcggccgtgg tcagtatggt
     8341 gactgctggc tcgaactcat tccgcaggaa cagggcaaag gctacgaatt tgaaaacaaa
     8401 gtcgttggcg gcgctattcc taaggaatac atcggctccg ttgaagccgg tgtcaaagaa
     8461 gcaatggaaa caggcgtttt ggctggtttc ccgatggtcg atatcaaagt cgtcgtatac
     8521 gacggttctt accatgaagt cgactcctcg gaaatggcct tcaagattgc tggctctatg
     8581 ggcttcaaag aaggtgcccg caaggcagac ccggttctcc tcgaaccgta cacgaaagtc
     8641 gaagttgtcg ttcctgaaga atacatgggc gacgtcatcg gcgacctcaa ctcccgccgt
     8701 ggtcgtgtcg aaggtatgga aatgcgctcc ggtgcagaac atatcaacgc attcgtaccg
     8761 ctggcagaaa tgtttggcta tgcaacggat ttgcgttccc gtacgcaggg ccgtggcgtt
     8821 tatacgatga cattcgacca ctacgaagaa gttccgaaga acgtagccga aaaagtcatc
     8881 agcgaaaaag gctaatataa tttaactcac actggaggaa cagcaaatgg ctaaagaaca
     8941 ttacgaaaga accaaaccgc atgttaacat tggcacgatc ggtcacgtcg accacggcaa
     9001 aactactttg acagctgcca tcaccaaagt tttggctgaa aaaggctacg ctaaattcga
     9061 agactatgct gatatcgaca aggctccgga agaacgtgaa cgtggtatta caatcaacac
     9121 ggcacacgtt gaatatgaaa cagacaaccg tcactatgca cacgttgact gcccgggcca
     9181 tgctgactat gtcaagaaca tgatcaccgg tgctgctcag atggacggcg ctatcctcgt
     9241 cgtttccgct gctgacggcc cgatgcccca gactcgtgaa cacatcctct tggcccgcca
     9301 ggttggtgta ccggctatcg tcgtatacct caacaaagct gaccaggttg acgatccgga
     9361 actcatcgaa ctcgttgaaa tggaagttcg tgatctcctc agctcctacg atttccccgg
     9421 cgacgaagtt cctatcgtag ttggctctgc tctcaaagcc ctcgaaggcg acgaaagcga
     9481 aatcggcaaa ccgtccatcc tcaaattgat ggatgctgtt gacgaataca tcccgactcc
     9541 gcagcgcccg acggaccagc cgttcctcat gcctgtcgaa gacgtcttca ccatcacagg
     9601 ccgcggcact gttgctacag gccgtgttga acgtggcgaa ctcaaagttg gcgacgcagt
     9661 tgaaatcgtc ggcctcgctg acgaaccgaa agatacggtt gttacgggcg ttgaaatgtt
     9721 ccgtaagatc ctcgaccgtg ctgaagcagg cgacaacatc ggcgctcttc tccgcggcat
     9781 cgaccgtaaa gaaatcgaac gcggccaggt cctcgctaaa ccgggtacca ttcatccgca
     9841 cacgaaattc aaagctcagg tttacgtctt gacgaaagac gaaggcggcc gtcacacccc
     9901 gttcttcaac ggctatcgtc cgcagttcta cttccgtaca actgacgtaa ctggcgtcat
     9961 ccagctcccg gaaggcactg aaatgtgcat gcctggcgat aacgtcaaaa tggatgttga
    10021 actcatcact ccgattgcta tcgaagccgg cctccgcttc gctatccgcg aaggcggccg
    10081 tacagtaggc gctggcgtcg tttccgaaat cgaaggctaa aaatagtttg aagtttgtag
    10141 taccgagttt gtagaaagag gttaaaattt ttagccaatg actgcaaact aaaaactaca
    10201 aacttgatac ttaaaagagg ctgtcgcata tgcgacagcc tcttttttgc atgcattcat
    10261 gcatgaatcg cagttcgcag tccgctgttc gcaaaaccat gccacatgga cccgttaggt
    10321 gtatggacat tcttcacagc gggccgtcca aggctccctt cataggggag ctggccgcta
    10381 aaggcggtct gagaggttaa aggacggccc ctacgcacaa ggccgcaggc cgtgaaagat
    10441 ttatcacctg gctgcgattg taggggctcg cacgtggcga gcccgcttct ggcataatga
    10501 gggaatcgcg gttacggtag cgtggggcac caaagcctcg cctcatagga gaggtggccc
    10561 gaagggccgg agaggttaac ctctcagtca gctgcgctga cagctctcct agcaggagag
    10621 ccgggccgct aaccactagc cactaactgc taacaactta aaagacttgt cgtcatgcga
    10681 caagtccttt agcatgatgc aacgacatgg gacccatgtt aaattggttc gattatttct
    10741 caaaaacata gaaaactcct tgcatgatgc gcctttttgt agtaaactaa gtaagtacgc
    10801 gacaacaggg taataccctc tgcggcgcgc taaactatga tataagatgt tgcccgcctt
    10861 gcggggaaac tcttatggag cagtctattc aaggaaatag acgttaggag gaattaagta
    10921 atggcaaaac aggaaagaat tcgtattcgc ctcaaagctt acgatcacaa aacgcttgat
    10981 cagagcgcgg cgaaaatcgt cgacacggcg aaacgtaccg gtgctatggt atccggaccg
    11041 attccgcttc cgacagaaaa gaacatcttc acgattcttc gttctcccca tgtcaacaaa
    11101 gactctcgtg aacagttcga actccgtacg cacaaacgct tgatcgacat tctcgaagca
    11161 tccaacaaga ccgttgatgc cttgacaaga ctcgatctgc cggcaggcgt agacatcgaa
    11221 atcaaattat aggaggaggt gcgattatgt ctaaagctat tttgggtaaa aaattaggta
    11281 tgactcaggt ctttactgaa gaaggcgcag tcgtccctgt aaccgtcgtc gaagcttccc
    11341 cgaacgtagt tgtacgtgtc aagaccgttg atacggacgg atacaactcc attcagctcg
    11401 gctatggtga aatcaaagaa aaacatctga caaaacccgt taagggtcaa ttcgacaaag
    11461 caggcgttgc tcctgttaag tatcttcgcg aattccgttt gacagatacg ccggaatata
    11521 cggtaggcca aactctggca gctgatattt tcgcaagcgg cgatctcgtc gacgtaattg
    11581 gcaccagcaa gggcaaaggt tttgcaggta ccatcaaacg tcacggtttc caccgcggac
    11641 cgatgggtca cggctccaaa tctcatcgtg aacccggttc tctcggacct atgaccagtg
    11701 gtggcggcgg taaagtcgtc aaaggtaaaa aacttcctgg acaaatgggt ggcaatcagg
    11761 caactgttct tcatctctcc atcgtcaaag tcgacatgga caagaatctg atcctggtta
    11821 aaggtggtat cccgggcgcc aaaggcagcc tcgtcatgat tcgcgatact gtaaaacccc
    11881 gctaatagtt gtctacagga aggaggataa aatataatgc caaaagtaag cacgtataat
    11941 attaccggcg cacagaccgg tgaaatcgaa ttgaacgata gcgtatttgg cgttgaagtg
    12001 aacgaagccg ttatgcgtca ggccgttctc cgtcagctcg ccaaccagcg cttgggcaca
    12061 cattccacca aaactcgcgg tgtcgttcgc ggtggtggca gaaagccttg gaaacaaaaa
    12121 ggaaccggcc gggcccgggt aggcagaaac cgcagcccgt tgtgggttgg tggcggcacc
    12181 atctggggtc cccatccgcg ctcttacgca cagaaaatgc cgcgtaaagc tcgtcgcctc
    12241 gccgttaaat ccgctttgag caataaagtc aatactaacg aattgttcgt cctcgacgaa
    12301 atcaatctcg cagctccgaa gacgaaagaa gtcgtcgcac tcatcaacaa cttcaaattc
    12361 gctggtgaaa aagtcctttt catcaccgac aacgatgaag tcgtagaacg ctgcgcacgc
    12421 aacatcaaag gcgttaaagc catttccact acgggcgtca acatctttga cctgctccac
    12481 tacacgaaat tgttcgttac gaagagcgct gtagctaaga ttgaggaggt gctcgcataa
    12541 tgaacatgca tgatctcttg ctgaaaccgg tcatcactga aaaaacgacc atgatgatgt
    12601 ctgatggcaa atacacattc aaagtaccgc tccgtgctaa taaagttgaa atccgtaaag
    12661 ctgttgaagc cgtcttcggc gtcaaagtta aaagcgtagc aacacttcgc acgatgggca
    12721 aattcaagcg catgggtaaa tacatcggca agcgcccgga ttacaagaaa gccatcgtaa
    12781 cccttcagga aggcgaaacc atcgaattct tcgaaggagc ttaaggaatt aacgaataag
    12841 gaggaggcac tacaatggcc attaaatcat ttaaaccgta ctccgctagc cgccgtttca
    12901 tgactgtttc gacattcgaa gaaatcacgg cgtctaaacc ggaaaaatcc ttattggcta
    12961 aagtaaccaa taagggcggt cgtaataaca gcggccgtat gactgtccgc catcagggcg
    13021 gtggtcacaa acgccgttat cgtttgatcg acttcaaacg tattaaagat aacatcccgg
    13081 caaaagttgc gacgatcgaa tacgatccga accgctctgc caacatcgct cttctgttct
    13141 acgcagatgg cgaaaagaaa tatatcatcg ctccgaacgg ccttaaagtt gacgacgtcg
    13201 tctacagcgg cccggaatcg gatatcaaag tcggcaacgc attgccgctc cagaatatcc
    13261 ccctcggtac actcgtacac aacgttgaac tgaaaatcgg caaaggcggc cagatggtcc
    13321 gttctgctgg tgaatcggct cagctcatgg ctaaagaagg caactatgca ctgctccgcc
    13381 ttccgtccgg cgaacttcgt caggtacacg tacgctgccg cgctacgatc ggcgtagtca
    13441 gcaatacgga ttatgaaaac attaccatcg gtaaagccgg ccgttcccgt tggaagggca
    13501 tccgtccggg caaccgcggt gttgtcatga acccgtgcga tcatccgcat ggcggcggcg
    13561 aaggtaaagc tcctgttggt cgtaaacgtc cggttactcc ttggggtaaa cctgcttatg
    13621 gcgttaagac tcgcgacgct aagaaagctt ccaataaatt cattgtgaag agacgtaaat
    13681 aagtgttgag gaaaggagat aactaagtgt ccagatccat taaaaagggc ccattcgtag
    13741 cacaccatct cttgaagaaa atcgacgcta tgaacgaagc caacgaaaag aaagtaatca
    13801 aaacctggtc ccgtgcctcg atgatcctgc cgagcttcgt cggccacacc atcgctgttc
    13861 acgacggccg taaacatgta cctgtctata tcacggaaga tatggtaggt cataaattag
    13921 gcgaattcgc accgactcgt acgttcaagg gtcatgctaa agccgaaaaa gtaactaaat
    13981 aagggggtaa cataaatggc agtaaaagct gttacgaaac acattcgcat tgctccccgt
    14041 aaaattcgga ttgttgctga cttggttcgg ggcaaaaacg tcggcgaagc tttcgctatc
    14101 ttgaaattca ctccgaaagt gggttcccgt gtagttgaaa aaactttgcg cagtgccgtt
    14161 gctaatgcgg aacataacaa tgatatggac gttgcaaaat tgttcatttc tgaaatcttt
    14221 gttgaccagg attccacgat gaaacgtatt catccgcgca gccgtggaca ggccttcaaa
    14281 attctcaaac attccagtca tttgacggtt gttgttgacg aaaaagcata aggagggaaa
    14341 tggagtgggt cagaaagtta atgcccatgg attccgcgtc ggcgtttcca aaccttggga
    14401 cgcaaaatgg tatgcagaaa aagattacgc tgcacttttg aatgaagacg tcaagatccg
    14461 tgaattctta aagaaacagt tatacatcgc aggtgtttct cgtattgaaa tcgaacgctc
    14521 tgcaaaacgt atcaaattga ttatccatac cgcaaaaccg ggcatggtca tcggccgtgg
    14581 cggcgcaggt atcgaagata ttaaaaaagc gttgaaagcc ttcaccacta agactgtcga
    14641 cgtaaacatc gcagaaatca aacaggccga aatggatgca accctggttg ctgaaaacgt
    14701 tgccggccag ctcgaacgcc gtatcggctt ccgccgcgct atgaaacagt gcgttggccg
    14761 taccatgcgt atgggcgcta aaggtatcaa aatcatgtgc tccggccgtc ttggcggtgc
    14821 tgaaatcgct cgcagcgaaa gccttcgcga cggttccatc cccttgcaca cgctccgcgc
    14881 taacatcgac tatggcttcg ctgaagctca tacgacatat ggctgcatcg gcgtaaaatg
    14941 ctggatttac aaaggtgaaa tcctcccgga agctaagaaa gctccgaaga aacgcgaagg
    15001 gagtgaagca taatgttaat tccaaaacgt gttaaatatc gtaaacagtt ccgtggccgc
    15061 atgaaaggcc aggcacatcg tggtaataaa gttgctcatg gcgatttcgg tctcgtcgct
    15121 ctcgaaccgt cgtggattac gaaccgccag atcgaagcag cccgtatcgc tatgacccgt
    15181 tatatcaaac gtggtggtaa agtctggatc aaaatcttcc cggacaaacc gatcacggca
    15241 aaaccggctg gtactcgtat gggttccggt aaaggcgctc ctgaatactg ggttgccgtt
    15301 gcaaaaccgg gccgcgtact ctttgaaatg ggcggtgtaa gcgaagaaat tgctcgtgaa
    15361 gcaatgcgtc ttgccggcca taagctgccg attaaaacga agtttgttgt taaaggtcag
    15421 gaattaggtg gtgaataaaa tgaaggttaa agaaatccgt gcgctgagcg ctgccgaaat
    15481 ggatgaaaaa gtggttagcc tgaaagaaga attgtttaac ctccgttttc aacatgcgac
    15541 cggccaatta gaaaatccaa tgcgtatccg cgaagtcaaa aagacgatcg ctcgcatcaa
    15601 aacggtacag cgtgaagcag aactgaaagc gcaggcgtaa gaaacgtcca taattggaat
    15661 ctaaaaggag gtcacaagca cagatggaaa gaaatgaacg gaaaatccgc gtaggtaaag
    15721 ttgtcagcga taaaatggac aaaaccgttg tcgttgctgt agaatataaa acacagcaca
    15781 agctgtacaa aaagccgatt atctcgactg taaaattcaa agcccacgat gaaaacaacg
    15841 aatgccatgt tggcgacgta gtccgcattg aagaaactcg tccgctgtcc aaacagaaac
    15901 actggagagt agtgacaatc gtagaaaaag ctaaataagc ttcattaatt aggaggttaa
    15961 gccatgattc agcaagaaac tgtgttgaat gttgccgata acactggcgc caagaaaatc
    16021 ttgtgcatcc aggtcatggg tggctcgtat cgtaaatatg gcaacattgg tgatatcatt
    16081 accgcatctg ttaaagatgc atcacccggt ggcgttgtca agaaaggcga cgtagtcaaa
    16141 gctgttatcg ttcgttctaa aaaaggtttg cgccgtgcag acggttccta tatccgtttc
    16201 gacgaaaacg cagctgtcgt aatcaataat gataaaagcc cgaaaggtac tcgtattttt
    16261 gggcccgttg ccagagaatt gcgtgaaaaa gatttcatga agatcatctc cctggctccg
    16321 gaagtattat aaggaggagg tgttctcaat gggtaaaatg cacgttaaaa caggcgatac
    16381 ggtcatcgtc atcgctggta aagaaaaagg caaaaaaggc aaggtcatcg caacgtattc
    16441 gaaaaaagac cgtgtcgtag ttgaaggcct taacctggtt aagcgccaca cgaaaccgag
    16501 tcagaacaat cctcaaggcg gcattatcac caaggaagca gcaatccatg tttccaacgt
    16561 aatgctcgta gatcctgaaa gcgacaaacc gactcgtgtc aaaatggtcc agcaggccga
    16621 tggcaccaaa gtccgtacag ccgtaaagag cggtaaagct atctaataag aagggaggcc
    16681 cttgagagtg tccagattaa aagacaaata cctttccgaa gtcgtaaaag gcttgcagga
    16741 aaaatacaat tataaaaacg taatgcagat tccgaaggtt accaaagttg ttatcaacat
    16801 ggccgttggc gaagctgtta ccaactccaa agccttggac gctgctgtca atgatatgac
    16861 catcatttcc ggtcagaaac cgatcatcac gaaagccaag aaatccatcg cagctttcaa
    16921 aatccgtgaa ggcatgaacc tcggctgcaa agttaccctg cgcggcgaac ggatgtatga
    16981 attcctcgat aaactgatga acttgacact gccccgcgtc cgtgacttcc acggcgtcag
    17041 tgccaccggt ttcgacggcc gcggcaacta taccctcggc ctgaaagaac agcttatctt
    17101 cccggaaatc gaatacgata aggttgataa agctcgcggc atggacatca ccatcgttac
    17161 ctcagctccg accgacgaag aaggccgtga ca
//
LOCUS       DAWCYS010000010        16630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_228_length_16630_cov_8.715716, whole genome shotgun sequence.
ACCESSION   DAWCYS010000010 DAWCYS010000000
VERSION     DAWCYS010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16630
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228_length_16630_cov_8.715716"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3458 a         4733 c         4847 g         3592 t
ORIGIN      
        1 ccggtgaccg taccaccggt aaagccgccg acaccgtgga tgatgaggcc ttcgtcttct
       61 tcttcgatat cgccgccgat tttgttgagc tctgtggcca tggcgtgcag acggtcacat
      121 tctttcaagc ggacccggcc ggcatggatg atgcgggtaa tgcccttggc acaggacgcc
      181 aggacggtca ggaccgggac caggtccggg cagtcttcga cgtcgatgac tcgtccagtc
      241 agttctgacg ggcaggccgt caattccgtc ttgccttcga cctggccgcc catgtcgcga
      301 atgagagaga cgatgacttc gtcgccctga gacgatttcg accgcagacc cgtgcagcag
      361 atagcccggc ccaggctgcc agccgagagc cagaaagcgg cctgggaata atcaccttcg
      421 acgacataag tcccggatcg atagtgctga ttgccaggaa tccgatagga gccgtcccat
      481 tctttatcga tgacgatgcc gtgctggcgc aggcagtcga gggtcatgtc gacatagctg
      541 gccgattcca gcggcgtcgt actgcgcagg accgagtcgc cgtcgagaag gggcaaggtc
      601 atgagcaggc ccgtgaagaa ttgagagctg acgtcgccgg ccagggaata cgtcccgggc
      661 tgcaaggtcc ccgatacggt caggggcagg gcgccttccg tcgtttcata agtgatgccc
      721 ttttcatcaa agagttcata atacggcgtc aacggccgtt tggccaggcg gcctttgccg
      781 gaaaaagtaa ccttattgcc gctgaggatg gcgacgggaa tgaggaagcg caaggtcgat
      841 cccgattcat tggcatcgac gaccagcggc tggccctgag gcttcaagcc gccttggacg
      901 cgataggcag cccggccgga atgagctgaa acggcaggtg tgatgtcggc ccctaaagcc
      961 ttcaggatat cacacgtcgc ttcgatgtcc tgggataggg agatgttgtc gaccaggctt
     1021 tccccttcgg ccagggccgc gcagatgagg tcgcggtggc cgatactttt agaagaaggc
     1081 gcttctaccg tcccttgtaa ggtatgtggt gtaatgatga cattcattgg gcgtccttct
     1141 ttcggatgta tttcggcagg tcttccgcag ccagcggcag caggcgggcc ttgccgattt
     1201 cttcgataat gaccagggta atatggttgc cgcgcatctt cttatcattg ctgatgtaag
     1261 ggatgagttc ttcagccgat gcatcgacat cgatcggcag gccatactgt tcgaggacct
     1321 tgcgcagccg cgccgtcgtc ccggctttcg tcagccccat ggcttcggtg acaccggtca
     1381 gcatggacat gccggcagcg acgccttcac cgtgggtgta gtggccaaaa ccataacagc
     1441 cttcgatagc atggccgata gtatgaccaa agttcaacat catacgttcg cccatatcaa
     1501 agacatcgtg ttcaacgata tcggccttgc tctggcaaca gcgttcgatg atttcttccc
     1561 aatgctcgaa aacagactgg tcatcgagct tttccaggag ttcaaagagg gccaggtcgc
     1621 ggatgcagcc gtatttgatg acttccccca taccgtcgtg gatgaagcgc ttgggcagcg
     1681 tcgatagcag tgccggatcg atgaggacgc ctttgggctg gtaaaaattg ccggcctgat
     1741 tcttgccgct gggcaggtcg atggcaactt ttcctccaac gctgctgtcg acttgggaaa
     1801 taatcgtcgt cggaatctgg ataaagggaa cgccgcgcaa gaaggtcgct gccgcgaagc
     1861 cgccgaggtc gccggtaacg ccgccaccga aagtcaggat acaatcgctg cgggtaatgc
     1921 cagcatcaga gagctgctga tagaggtcta ggagggtcgc tgcgcatttg tgttcttccc
     1981 cggccggaaa gatcaggcgg tgaacgcgga agccagcctt tgttagaacc gtttccaatc
     2041 gctgaccgta gagggcatcg acgttcgaat cactaatgac agccaggttc tgcgatttcg
     2101 tcaggttgcg gatataatcg ccagcccggt cgagaaggcc attttcaata tggatatcat
     2161 aagagtcttt tcctaaatca acgtgaatgt gtttcatgaa ctaattccct tctccgcata
     2221 gcggcttgct gtaaaaaacg ggtcatctct tgggcatctt cttcggccag catggtcgag
     2281 aaagattgca ggactgcgct gaagcggttg atttcataca agaggttgtc tttattggat
     2341 aagaacagct tggtccacag cggcgcattg atgtcggcaa cccgcgtccc atcgcggaaa
     2401 ctgccggcta caaaatactt ggtcagcgta ttcatggatt cgctgttgac cagagccgtc
     2461 gccaggacgt ggggcaggct gctggtataa gcgatgaagc ggtcgtggtc ttccggcgtc
     2521 acgcggacga cgtgggaaca gcccagggcc atggccatat ccttcacagc ccggatatgg
     2581 tcttcgtcgt tgcccgccat ggggacgagg atataattgg caccgttgaa gatatcggca
     2641 ctggccatgg catacccccg gccttcacgg cctgccatgg ggtgaccggg cacgaaatca
     2701 acgccttccg ggagaagagc ggcaatagcc gtcgccgtat cccctttgat accggctacg
     2761 tccgtcaaga ccgcccccgg tgcaaagaag gctttatttt ctgccaggaa tcgtatcatc
     2821 acatcagcgg ccgtacaaaa gatgaccaga tccgcctgct tcaatgcctt actgccagcc
     2881 gtatagattt catcgaccat gccttcctgc ttagcctggt ggagggcttt ttcatcggaa
     2941 tcgatggcga tgatatgtcc gacgccgatg cgccgcaggc cgcgggcata ggaaccgccg
     3001 atgaggccca ggccgacgat ggccacagtc tgtttcgtat taaaaccact agcttcctta
     3061 tccatgattg tctccccctg tatcccttat atttcccggc cgacgacggg tgccagtttg
     3121 cggagcgaat ccatgaggtc ggcgaacatg gccggcgtaa tcgactgagc gccatcgcac
     3181 caagccctgt ccgggtcgtt gtggacttcg atcatcaggc cgtcagcccc ggcagcaacg
     3241 gcggctttgg ctaggtcggc taccatccag cgcttacccg tcgcatggct cgggtcgacg
     3301 acgatgggca ggtggctctg gcgtttgacg gccaggacgg cactcaagtc gagggtgttg
     3361 cgggtgtacg tttcaaacgt ccggattccc cgttcgcaga ggatgacgtt gtggttgcct
     3421 tcggccatga tgtattcagc cgacatgagc cattcttcga tggtcgcgct caggccccgc
     3481 ttgagcagga ccggcttgcg cgtcttaccg acggctttga ggaggtcgaa gttctgcatg
     3541 ttccgggccc cgatctggat gatgtcgacg ccatcttcga ggaagaccgg cagtttgtcg
     3601 gccgacatga tttccgtaat gaccggcagt ttcgccttgc ggccggcgtc gcggagcatg
     3661 tcgaggccct tgttgcctaa accctggaag gagtacggcg aagtacgcgg cttgaaagca
     3721 ccgccgcgaa ggatgttggc accggccttg gcgacgtcgt aggcgacgcc ttcgatctgg
     3781 tccggcgttt caatggagca gggaccggcg atgacggcaa gtttcttgcc gccaatggtc
     3841 acgccgctgt taccgacggg aatgaccgaa tcctggggat ggaacgcccg gctggcccgt
     3901 ttgtacggga cttcgacgcg catgaccttg tcgacacatt catagctctg tatctgttcg
     3961 acatcgacga cttccgtatt gccgacgagg cccatgacag tctggcgggc gcctttactg
     4021 atatcgacct gcaggccctt ggccttgatg gcatcgacta attttttcgc ttcttctttc
     4081 ggtgtgtttg ctttcaatac aatgatcata actttttcct ccattccagc aacaaaaaaa
     4141 gcaggctcac atgagcctgc ttctaagatt ctcgtttatc gccttttcct cgcgtcttgc
     4201 ttaggcagcc atagccggct ggcacggaaa aggtggaagt cttctcatgc ttacatcggt
     4261 tttattgaat tcgtcgtagc aaaaataacc agcgctaaaa tacaggtagc cccagctaaa
     4321 ataagtaaag acgtattcaa ttacctgatg taagaatgac gtgttcatag gaatctcctt
     4381 gtgcaacatt taaaaattcg ttgtcgtcat tgtatacccc ggggaaataa ttgtcaaggt
     4441 aaaaaagcag ggattctctg tatagatatg agaatccccg tggacaatcg ttttactgac
     4501 ggatatattg tatactattt tcgcatatca tctatgtctt ttaccaagaa ggcggaatgg
     4561 ctgtcgtgac gacgcggaac gtatagccct gccctttcag gagatggatg atgtccggca
     4621 gggcgtcgac ggtcgcaccg tggccatagg catcgtgcat gaggatgacc agatggcgat
     4681 ggagataggg ccgcaattgc agctgctgct ttatgttttc cacttcttta tcggctgtct
     4741 tgcccgtccc gtcagcatcg ccggtcaagg cattccagcc gacttcgata tagccgatgt
     4801 tgttgatagc cgtccagaag tccttggtga agtgcccttc cgtaccgcca ggggcgcgag
     4861 aaatgatggg ccggacgccg agaatcttat aaatttcttc ttctgtctgc aagagctcgt
     4921 ccgtataagc cttgggcgaa acgtatattt tcttataatc atgcgtatac gaatggacgc
     4981 cgatggcatt gccagactgg tagattttct tgaccacatc agggtagcgg acgacattct
     5041 ggccggtcag gaagaaggta gcatggatgt tttccttttt caggatatcc agaatgcgcg
     5101 acgtattctt atcgttcggg ccgtcgtcaa aggtcagata gacgacgttg tcaccataat
     5161 aataatcccg cgtcttgccg tatgtcgtgg gcaggccctt ttcctgacgc tgccgggtcg
     5221 tatatttatc gtacaccggt ttatcattat cgacaggagg cagggagaaa tcatcaccga
     5281 tcagagctac gtcaggcttg ctggaaagga cctgcggcac cgggatatgc cgggcttcgg
     5341 ccgctgccat gacgcggtcg cggtcgaaat tcaggaaaag ccccagcgaa gccaggaaga
     5401 aaagtaagat acatcccaaa aacagtctgc ctttttgttt catttttgct tacatccgtt
     5461 tctttttgac attaccatag tgaatatact ggcgtatcgt acacgtttcc cgatagccat
     5521 cggccaggac gctggccgtc aggtcgaagt cttccaaatc gtcgaggagg gcttcggtca
     5581 tgcggtgctg gacggcatta tagcccgtgc agaagaggat ccgcgggcag cgcacgccgc
     5641 ggaacagtcc ccgcggatgc ttgatgagct tggccagcat ggccggcgta atgacagcgc
     5701 cgcaaggacg gcccatgatg gccgctgccg tttcgacgcc ttcgattttt tcggcgctga
     5761 tgacgttgcc cagcatctgt aaattgagga caccgatgac ggtatcgcag gaagccggca
     5821 ggggtacatc gtctgccagc ggggccttga gccacttatt gccggctgca tcgccttcga
     5881 tgaggatgta ccaggccggg tggcgcatct tgatgtcatc gatgaccttg gggtcgaggc
     5941 cgatatagcg gtcctggtcg tcgaggacgg cgaaccacgc cgggacgtgg ccggcggcaa
     6001 tgcgttcagc gcagaaggct tcgccctttt ccatatcttc cgtgcagatg acgtcaaagg
     6061 gttcgacatt atcgacacgc gtgctgtcca tgggaacgat gctgctgacc atgatcggca
     6121 gatgaccgcc gtggccgtaa gtccccagcc gttccaatac ggtcgtctta ccaccggcac
     6181 cgacaatagc cgtaatgccg ggtttcaaca tacgtcgcca acgattatct tcaaacatga
     6241 aagtaccccc ttctggaatt cacttcttca caacataacg attatagcct ttccaggccg
     6301 atttgatgag gccgcggacc atatcgatgg tcagggcccg ccgttcttct tcggagcagg
     6361 tgccgaaatt gccgaaatcg agttcttcac tggccagatg ggccacattt tctttgacgg
     6421 ccatgccctt ttccgtcagt tcttcactgg ccgagcgggc aatggcagcc aggccgggac
     6481 ggccgccgac gacgatgcac cacgaatctt cccgcatggc gacggcaccg accagatacg
     6541 gttcatctgc cggcagcttg cgataggctt tataggccgt atagacagct tctttggcaa
     6601 tggaaatgcc cgttcccagt tcacccaggg gcgggcagtg caaatagtca tccaggctca
     6661 tgcagccttt atcctgcagc tgtacatcga cgtggaggga caggaggatc ggcaggagga
     6721 cggaaaaagg cgctttcccc gaaatgacgc cgcccagcgt gccgtagcgc tggacgtttt
     6781 tatcggctac gtcactgatg gccttgctga tggctccgcc ggcaaactcc tggatgaccg
     6841 gggaaatctt gatctgccaa agcggtgtca tggcgccgat gacgatgtcg tcttcccgtt
     6901 cttcgatata ctgcatcccg gctttcgaca aatcgacagc caggtcataa tggtcttttt
     6961 tatatttcaa gccctgcgtt cccgccagga aaacagccga cgcagcctgc cgataagcag
     7021 cgtaggctgc ggccggatta tccgggacca gggtcgtcgt actggtaatc acgatactga
     7081 aaatctcctt tctgcctagt taagggatgt cgtcgaagaa ccagtcttcc agggccacgt
     7141 tcggcctgtc cttgttgagc aggaaagacc cgccacattc atcgacgaca gcatcgacaa
     7201 gggcgtcgac gccgatgtcg ctgcacaaat ccatttcttc tttatataaa aggatgggca
     7261 tgagaagttc caccgtcgta ccatcggcca tggaaacgcc gcccagctga ccttcaaact
     7321 ggacgatgac caggccggac agagccgtat ccctggcaaa aggctcatgg ctttccgagt
     7381 tgggcaggag aaagccatag gacatccaca catcatggac gtgcgggaac cgccccagct
     7441 ccatgagcag ccagatgggc caggcttctt cgtcgtttag ggaaatatcc cagtccttgc
     7501 ggaagggcca atcagccggc aggatgaggc agagttcgcc gtagccttct ttcccggtca
     7561 caaattcgcc agccgggtaa tgcatgggcg cggcactcat gccgatggta tgaaggagat
     7621 agaagggttc ttctaccgta ggatataaga gctctacgtc cactgccagc ggcgtcccgt
     7681 ccgggtcctg gaagacactc cactgccgat gaggaaagag gctgtggaaa taagcctgga
     7741 cggcgcggcc atattcctcg tgaggccgct gtatcttcaa ggtgtccatc aaggaaaaaa
     7801 agcgaggcca tcgataggac gtaatcggta aggtactcat cgaatccccc cttttcgtta
     7861 tattcagctt cattataaca taaatgagcc cgctctgggc aaccaaaaag gggcgccgcg
     7921 aacgcgaagc ccctctccac tatcatgacg atagactttt tagccctcag ggttcgtgcc
     7981 ggagggattc actgaatctc atcgcctgtt caattgacgg gatatatcat atcaataaat
     8041 agggaaattg tcaaatcaca gcgccttcgg cgcgccttgt aaaaggcagg acggtatagt
     8101 agaataaagg agaatggaac gcactatatt aaccgcaaag ggatgactac attgaaggta
     8161 cgctgggtag aattaaaccg ttttgaagct gaactgatcc tcgaattcgc ctgccagccc
     8221 aaggatgctc ccgtttctgc cagcctgtgc cgtatgatat accgccgtct cgaaggccag
     8281 ccggcactgg cacagaacca gccgggactc caggacgggg aggcgtaaaa ggcagcctca
     8341 ctcttccatc gctttttcta atcgagcggc gatggccttc cccgtttcgg tccgggccag
     8401 gccgccatcg gaggtttctt tcagggcgac tggcatgagg cggccgattt ccgccatggc
     8461 cgcaatgact tcatctggcg ggatgacgct cttgatgccg gccagggcca tttcagcagc
     8521 cgccagggct tcaacggcaa agacggcatt gcgcttgaca cagggcactt cgaccaggcc
     8581 cgctacggga tcgcaggcca ggcccagcgt gtttttcatg cacagggcaa aggcctggag
     8641 gatcgtatcg ttatcgccgc cggccatttc ggctacggcc gctgccgcca tagccgcagc
     8701 cgtcccacat tcggcctggc agccgccgac ggcaccggcg acagaggcat tgcgggaaac
     8761 gacggcgccg atacccgctg ccgtgaacaa agcatcgacc agtacgggac ggggcgtctg
     8821 catttcttcg gcagcggcca tcatgacggc cggcacgatg ccgcaggaac cggctgtcgg
     8881 gcaggcgacg attttgaaca tcttggcatt ggcttcactg acggccaggg catagacagc
     8941 cgccttatgc gccaaggggc ccagtaaatt cggcgccttt tcagccagtt tatgggcatc
     9001 gccgccgacg aggccgctga ccgattttcc cgggtccttc atgccggcat cgatggattc
     9061 ctggaacaca tcgagacggc tggccatgat ggcccgtact ttttcttcca gttcttctga
     9121 cgactctact tcatgacgca cgacgatgtc tgacagagtc gtcttttctt tttctgccag
     9181 ggccaataag tcatgaaggg tcttatattc atatttatac atcttattcc tcctagagcg
     9241 gcagcagcgt gacgacgctt tcaatggctg taatattctg tatcatgtgg acgatttcat
     9301 tgggaacagg gctgtccgtg cagacgatca tgacggctgt ttcgttcttg gcactgcgga
     9361 agacgcgcat attcgataca ttgatatgac cgatggccag gaccgtcgtc gcctgggcga
     9421 tgatgcccgg ctggtccctg tggaccgtaa tcagtgtatg ttcttcccca ttcagggcca
     9481 cttctgcccc gttgatttcg cggatttcga ttttaccgcc acccagggag acgccgagga
     9541 cttccatctt ccggccagac gcgccggtca tggcgatgcg taccgtgttg ggatggtcca
     9601 cttcttcatc ggaccggtgg aattcaatag ccaggccctg gtctgcggca atggcaaagg
     9661 acgtgcgcag gcggacatcg tcggcagaaa agccgagcag gccggcgacg agggcccggt
     9721 ccgtaccgtg acccttatag gtcttggcaa aagaaccata gacagtgatg accgcctttt
     9781 tcggttcatc tttcaggatt tcccgggcca tgaggccaat ccgggcggca cccgccgtgt
     9841 gggaactgga cggcccgatc atgacagggc cgaccacatc aaaaactccc atgagaaaac
     9901 ctccttacag tttttatagt ctgatttctt gttgtagatg taagctataa atgtacgttt
     9961 cttcgacggg ccggtgcgta atctgttcga cagcccgctt gtataatgcc agctggacgg
    10021 catagcgtct gcggaaggcg tctgccgttt ccagccggtc cgacttataa tcgacgagga
    10081 tccaggcatc atcttcgcgg aaaaggcagt cgatgacccc ttggatcaaa atctgttcgc
    10141 ccgtttctgt ctcaggcaga ggatcgccgc cagctaacag gacagtgaag ggatattcgc
    10201 gatgcagctc cggcgattcg gccatgcgcc ggcctatatc ggactgacag aaagctgcca
    10261 ggaccggcac atagaccagc ttggcctctt cccggctcaa caggccctgg cggacaaagg
    10321 cgtcgatttc acgacgcaaa gtcgccgtcg tctgatcggc agccggcgtg atatactgca
    10381 aggccttgtg cagggccgtc ccgcgctgag ctccgctgac agccgcttct tctccggcca
    10441 gccagggcgg cagggcggca aaggcatcgt cctcaccggg cgccggcggg atgacggccg
    10501 cagccgggac agacagggcg gcttcgtctt gcagttcatc gctgtgcagt tcctgatatc
    10561 gccgcttgac ttcggatacg gaaaacttgg ccgctgtccg gacagcctgg ggataggcat
    10621 actgccagga taactgttcg tccagccagg acgggacagg cgtgccggtc aagtcgccgc
    10681 gccgcagggc ttccagccgc ggttccccat cataggcgcc ttcttcaaca gtccctgccg
    10741 ggacggcctt ggcgatgcgc acctgccaga ccgcatcctg ccaggccgca ccgggacggg
    10801 cataatcagc atcggccttg gccccgaagg gggccagggc agccggcatg acccagtcga
    10861 aatacgattt ggcctgagcc ggattgaggt ggctcgtcca gcgctgccag tcacggtcga
    10921 tgtggctgct gtggcccgtc aggataagct ggtccctggc gcgggtcatg gcgacataga
    10981 ggatgcgctc ttcttcggcc gtcccttccc agcgcagctg ggcggcgata ccgctccaga
    11041 tgagcgtcgg ataggatatg cgccattccg ggtcatactg cttgagaccg atgcccaggc
    11101 gattgtgaaa gagtatctgg tcctgcgtgt cgcggcggtt gaaggccttg cccatatcgg
    11161 cgacgaagac gacggggaat tccaggccct tgctcttatg gatactcatg atgcgcacga
    11221 cgtcttcctt ttcactgacg actttagccg gcgccaggtc caggccgtct tctttcatct
    11281 tatccatgag ctgcaggtag cggaagaggc cgcggaagcc ggcttcttcg tactgccggg
    11341 cccggtcgta cagggctttc agattggcct ggcgcacgtc gccgccaggc atggccccga
    11401 cgaagtcgac ataagccgta tcatcataca ggcgctgcaa gagttctgcc acgccgtggc
    11461 ggcggctgta cgtgcgccac gaatcaaagg cggccatgaa ctgctgcagg tcctcttttt
    11521 catctacgcc gtcgggcagc gaagcaacaa aggccggcag gttctgccac agcgtcccgt
    11581 caccggccag gcggacaccg gccagggccg tctcatccag gccgaccaaa ggcgaccgca
    11641 ggacagccgc catggccagg tcctgttcgg gattatcgat acagcgcagg agggccagca
    11701 tgacctggac ttcgacggcg gcaaaataac cgccactctg ttcggcatat gacgggatgc
    11761 ctccttcctg cagggcctgg atgaggacgt cggccttgcc ggccatggag cgcaggagga
    11821 cgacgatatg gcgataagac aagggctcca gcgtgccgtc tttgcgggct gccatgcggc
    11881 cgctggccag gagctcgccg atgcggccgg caatgaagcg gcattccttt tcaaaggccg
    11941 tgctgccgtc gtcgtcttcc tcgtcactcg gcgtcggcac gatgtcgact tcgacaggcc
    12001 cgccgatcca gcgttcatcg tccggagcct gccggccggc gtagagtttt tcccgttcac
    12061 cataatccat gccggccgct tctgccgtca tggcccgcga gaagacagcg ttcacggcgt
    12121 cgaggacgac cggaacgctg cggaaattcc gccccaggtc gatgcagcgc tgcacggctt
    12181 tttcatcgcg gctgaaagac tggtatttct ccaggaacag cgtcgggtcg gccaggcgga
    12241 agcgatagat gctctgcttg atgtcgccga ccatgaaacg gttgtcctca ccggaaacga
    12301 ggcgggtaat gagttcctgg acgccgttcg tatcctggta ttcgtcgatg aagacctctt
    12361 catactggct gcgcagctct tcggctgccg ccgacggcac gggatgttcc ggcgatgcat
    12421 cgggggcgag gaggatctgc aggcagaaat gttccaagtc gctgaaatcg atccagccct
    12481 tttccttctt cgccgcccca taagctttgg caaaatccag ggtcagcgtc accaggccat
    12541 ccatgacggg tttcatggcc cgcatgccgt cgagccattc ttccggcgtc gccgagaaat
    12601 agacggactg caaggtcttg atcacgtctt tcttgacatc gtcacgcact ttcttgcacc
    12661 gttcccagac ggctttatcg tcatccgata atttccgcag tgacttcagg cgcgggaagt
    12721 cgatggcccg gacaaagcgg ctcaaatcat tccaggaccg ggcctgggcg gcctgccgga
    12781 gagctgcctg ttcagccacg aaggtatcgc aggccggcgc aaaggcttcg cgctggcgca
    12841 ggtgatagag cacgccgtca tagaggtggg catcttcttc aaggatacgg cggacggcat
    12901 ccttgatggg gccggcccag accatatcgt cgatgacggc gtccggcgcc acgtcataag
    12961 ctcgggctgc ctctttcagc caatgttccg gccaggccag gctgcgggca taggtataca
    13021 tgcggcttac tgtatccatg aggatgtcat cgccgcggtc gctgccaaac atatcggcca
    13081 gcggatagag gatggacgct ttctcatcgt cttcatagta agacaagaag acgtcttcca
    13141 ggacctggcg gcgcaagagg ttcaattctt cttcaccggc aatggaaaaa gccggatcca
    13201 ggtcgatggt atagaaatac ttacggatga catgctgaca aaaagcatgg agcgtcgaaa
    13261 tctgggccga tggcaataag gccagctggc gttcgacgcg gggatccttc gtttccgaca
    13321 aggccttcat cagggccgtc ccgatgcggt cgcgcatttc ggccgcggcg gccttggtaa
    13381 aggtgacgac cagtaattcc gtaatatcaa tgggatattc cttgtccagg aggcggcgga
    13441 tgatgcgttc caccaggaca gccgtcttcc cggaaccggc cgccgccgac aggagcagcg
    13501 tctgcccccg gctgtcgatg gctgcctgct gctcattcgt ccaggccatg ttcgtcatct
    13561 ccttcatcaa acatcttttg aatcatctcg tctgtatctt cccgtcccgc cgcgtcatac
    13621 gtattgccgg caacggcgac atcgaaccgg cagaccggcc ggtaagggca atagctgcac
    13681 ggcgcctgct ggcccatgag gatgggatgg atggaaatat caccgtcgcc gatatggccg
    13741 gcaatatcgc gtatctgccg ggccgcccag tcgagcaggt ggctccagcc tgcttcatta
    13801 tagagcgtgt gggacgaatc gctcagggtc ccgtcttttt tcagccgcaa gttgaggaaa
    13861 tcggaaaagc cctgcatcga cgtatccagc tgctgcatga cctggccgtc gtcgaggtag
    13921 aaaccggtca gcttcccttt ggcatcgtag agtttcttct tgtcttcctc actgacgcgg
    13981 tattccaggg ataccttatc attgcggacg taacagtata ggacggcagc cggaatggca
    14041 tcgccgccaa tattcagcaa agccacgaac atatacgtca aaagctgcag ttccaggccc
    14101 gtgaaaatct ggctgatgtc gagctgtttc cggccggact tatagtcgat gatgacgacg
    14161 tatttatggt ccccctggcg caaagtatcg atgcggtcga tctggccggt gacgacgact
    14221 tgcaggccat tcttcaaggt aaagcgcagg gcttcccagg gactgcccgg acggccgaag
    14281 gatttttcca gcccttccat atggaaatcg gaaacggcac tgaaagcgct gaggcgtcgt
    14341 accgtccggg tcagggtctg gatgagccgt tcctttatct gggcgaaata ggcgttggac
    14401 atgaggatat cgtgctggac ctgcggcgcc agttcatccg tcgcctggcg gcagatggcc
    14461 gggacgtctg cgtcgggaat gtcatgccac tgtttctgtt cccgcagcag ccgttccccg
    14521 atgatgcgca gggcgccgtg gaccagcatg cccaggtccg gtgcagcgaa ctgatagcgc
    14581 ggccgttctt ccaggccaag gccatagcgg gaaaaataag caaagggaca gctgcggtac
    14641 tgctcgaact tagtgaccga cccgcgcagc gtcccgtccg gcgcaaagag gcggcgcacc
    14701 aacgattccg gcagggtgac aggcaggttc gtatggaaca ggccctggac ggcgcgcacg
    14761 gcctgatggc gccagccatg ctgccaggcc cagtcataga gggcccacca tatatccccg
    14821 acagcttcgc cattggcagc cggccgcaag tgaccgggaa ggtaatcgag agccgccggc
    14881 agggccagca ggtaatcccc ttcccggccg ggctgtacct tggcctcttg gcggatgatg
    14941 tcagctacat agcctttatc ccggagctga tggatccacg acgctttttc catggcactg
    15001 ccgtcatcgt cagcgacgac gtaggatatg atgaaccgtt ccgcagcccg ggtcgcccct
    15061 aaatagaaca agaagcgttc ctggaaagag cggaagcggc tccccggtcc gaggacgatg
    15121 cccagatcag ccaggcggtg ccgttcttta tcgctgaaca gcccttcttc ctggctatgc
    15181 tgagggaaga cgccgtcatt gacaccgcag agaaagacga ccttggcctg catcgtatag
    15241 cctcgttcga cagccgtcaa agtgacgtga tccagcgtcg gcgggataag gctgaacttc
    15301 aaattggcca ggccatcttc gacgatttgg gaaaattcat cgaggtccgt ttcatcgtca
    15361 ccgcaaagac tgacgatttc attgaggaac gtcccgatac gcttccagac ctgctcgtgc
    15421 tccttgcctt cttcggcaag gcccgactgc tcatcttctt cctgccactg gcgcagtact
    15481 gacgggatgt ccagccggca cagccagtga tacaacaagg tacaccagtc gcgcaggcta
    15541 tgttcttcct gggcttcttc ccagcagggg aacaaaatgt cccgcacgcg ctggcgtatg
    15601 gcattcatgc gctggaggtg cgcttcttcc cgttcgtcga tgacctgggg ctgttcctgc
    15661 cggcggccgt aagtccactc agcatccctc agccagtgac gcccctggat gccgtaagcg
    15721 aggcaatagt tttccaaatc gtcgacgtca tggcggctga cgggaaagag gtccgtcttg
    15781 agcaggcgga agagcgactc atgagaccag cggctgcgga agacgccgaa cagggcggaa
    15841 atggcctcgg cggcgggatg gctggtcata ggccggcgat agtcgctgaa gcagggaatc
    15901 ccatagcgcc ggaaaatgcg ttcgacccgg tcgtggtaca agtcactcgt ccgggccagg
    15961 accaggaagt cgcgataacg atagccctga cggcagagcg ctgcgatttt ccgggcaacg
    16021 ctgtcgattt ctccatccct gtcgggacat tcccagattt ccagggccga tacgggccgg
    16081 tccatgctgg ccgggacggt ctggaaaaaa gcatcgcgaa aggccgtcag cgggccggcc
    16141 ggccgctttt cgacgacagc cgtttccata tggggaaaga gtgtctttat atcgcgatag
    16201 acttcgtaag cccggtggaa caaggccgtt tcccgcagct gctgtgccag atgcgcttca
    16261 tcgatggtca gggtaatcgt caccttcgag gctgcgcgct ctatctgccg caggatttcc
    16321 atctgctgcg gtgtgaacca ctggaagcca tcgacccaga catgggcgcc ctggaggaag
    16381 gaataatgcc cgatttcccg ggccagcaga gtcatcgtat catctgccga gccgaagtgg
    16441 tctgccagaa aggcctgata cgcttcatag agccgggaaa tatcttccag cttgtgcgac
    16501 agggtcgggc tggggacggc ttcagccgcc tggcgcaggg cttccggcgt aacgcagaaa
    16561 gaccggcatt cccataagaa gcggcctgcc gtatcggcat acttcggctg cctggccgcc
    16621 gtctgcaaga
//
LOCUS       DAWCYS010000011        15520 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_250_length_15520_cov_6.351891, whole genome shotgun sequence.
ACCESSION   DAWCYS010000011 DAWCYS010000000
VERSION     DAWCYS010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 15520)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15520)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15520
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_15520_cov_6.351891"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3734 a         4140 c         4260 g         3386 t
ORIGIN      
        1 ccaggtcttc tttggggatg ccgatgcctg tatcgctgat gacatagcgg acgggctttt
       61 cgtcggcctg gcaggtcagg gtgacctgtc cgccggcagg cgtatatttc agggcattgt
      121 cgacgaagat gcgcagcatc tgtttgacgc tgcccggttc ggccgtaatc agggcttcgt
      181 cgttttgacg caggatgatc tggtgttctt tgtcgatgag gctgacttcg ctgtatactt
      241 cctgcaggag gtcggccgta ttgattttcg taaagtgttt caacagcgtt ccattattgg
      301 aacgggcaaa gaagagcagg cgttcgatga gctgcttcat atagtccgtt tctgatttga
      361 tggccgtcag gctttcgttg aggacggctg ggtcgtcttt gccccagcgg cataagaggt
      421 cggcgtagcc ggcaatgacg gtaatcggcg ttcgcaattc gtgggacgcg tcgctgatga
      481 actgctgctg ttgtttataa ccgtattcga tgcggtccag agcctggttg atgctgacgg
      541 ccaggtcgtg gagttcgttc ttgttgtcag acagggcgat acggttgtcc aggcggttga
      601 cttcaatggt cttgaccgtg tcattgatga cctgcaaggg ggccagggag cgcttcatga
      661 ggtacatacc cgtgaagatg gtcaagatca ggctgatgag gaccgaagcc aggagctgtt
      721 tcgataagag cgacacgaag cccgtttctt tatcggggcg ctgagaaaag cgcaggtagt
      781 agttctttcc ctgtttgtcc gtccagagct gataatagga gaattcagac gattcgttgc
      841 cttggacgct ggtgggcagc ggctttttct gcggattgct tatggcgtcg tcgatgtagc
      901 ggtcgctcag tttgcggacg gcatgggtcg ggccgttgtc gacgatgaga tggcctgcgc
      961 cgtcataaat ttgtaagttc atcgacggtg gtacgttggc ccggacaaat atcgtcgtat
     1021 cgacctttcc gtagtcgtcc aggtattgga tggccgccgc agcgcgggac ttcaattcat
     1081 cgcgcaggga gtcgttcatg atgtagtgga cgctggtaat ggttacgacc gatacggtca
     1141 ggaggatgaa aaagacgata gacgaataga gcaggaccat tttagcgaaa atgggcaggg
     1201 gagcgatgag caggtgctgt gtcagccaat gattaatctt ctgccacata accgactcca
     1261 cggactgtac ggatgtagtt gcgcccgttc gggcccagtt tgctgcgcag gtaacggata
     1321 tagacgtcga cgatattcgt gtcgccgatg taatcgtagc cccatacctc ttcgacgatg
     1381 cgcgaccgcg agaggacgat gttcttgttc cggactaaat aggcgaggag ctcgaattct
     1441 ttcttcgtca attttaattt tttcgttccc ttcatgactt cgtgacggtc gaggaataaa
     1501 gtgatgtcat tcagctggag aaaatttccc cggtcggcca gctgatggcg ttctcgcagg
     1561 aggacacgga tgcgggcaaa gagttctttg ctgttgaagg gtttggtcag atagtcgttg
     1621 gcgccgatgt ccaggctgac gactttcgtt tcgatatcgt ctttggctga gagcatcatg
     1681 atcgggacgt tcgatagctc ccgcgtccgc ttacagatgg tcaggccgtc gacgtcaggc
     1741 agcatgatgt ccagcaggat gaggtcgaag tgttcctggc cgatgcggtc cagggcagcg
     1801 gcgccgttgt attcgatgcc acattcatag ccttcatgtt ccaactccat ttgcaggaag
     1861 cgggcgattt gtttttcgtc ttcgacgatt aagatatgtg tcttttcggt catataacaa
     1921 cctccccaaa aaagaatttg cacactctaa gccatataca tactattata acgtataaat
     1981 tccctttttt gtactactca ttcggacatt gttacaaaaa tatcacgaaa atttaatcta
     2041 tttttaatct ttagggattt acttttcgcc agaacctgtt attataatat atgtagaaca
     2101 gatcatctac gagacgttgg agaagtaagt tgttcatata cccctcgaac acccctatct
     2161 tattttcttc aactgctgag atattgtctt cccccgatga tatcttgcaa aaagagaaag
     2221 ccggctcccc tcgccggctt ttttctattt ctacggaaaa tgattttggc gaaattttga
     2281 gaaaattttt ttggacaatc gcaaaaacct aggaaatacc tatatcttta cccattcggt
     2341 attcattgac aagtctgtct attcgtgtaa taatgaggaa gacgaagggg agattccccc
     2401 gaaggcttga tgtttggagg tgaaaaccat gcatgttatt tccgacgaat gcgtaaaatg
     2461 cggcgcttgc gctagcactt gcccgaccgg tgccatcgaa gaaggcgaaa cgaaatacgt
     2521 tgtaactgat tcctgcatcg actgcggtgc ttgcgaatcc gcttgtccta caggcgctat
     2581 tgcagctgaa taattgcttc ggcaaaaggc tgtcgcagaa ggctttggcc atcatgcggc
     2641 ggcctttttt tattgaattt tccctgtttc tgtgatagga taaaagcact gtaagatgca
     2701 taataggagg agtttgcttt gtatgtatta ggcattgccc tggccgtgac cgttgtcgat
     2761 cagcttacga aatacgctat ccatcaggcc atggtccttc atgaatccat tccggtcatt
     2821 ccgggatttt ttcatattac ttatattttg aatcgcggtg ctgcttttgg gattttagaa
     2881 aatcagcagt ggctcttttt agtcatggcc ttggccttat tcctgctgta tggtctattc
     2941 cgcaagaaat tgccggcgac gccctgggtc tatggcgggg cggggctgct tttgggcggt
     3001 gccttgggca atgccctgga tcggacgctc cgcggggcag taacagattt cttcgatttt
     3061 cgcatctggc ccgtattcaa cgttgccgat atcggtatcg tcgtcggcgt cgcacttttg
     3121 ttatggtata gttggaccca tatttcggat aaatgaggat gaagacatgg aagaaaagaa
     3181 attaatagtc acagaacagg aacagggaaa gcggctggac gtctatttga cgacggccct
     3241 ggatatttcg cgcagttatg cccagcagct cattcagcgc aaggaagtca ctgtcaacgg
     3301 taaggaagcc aaggctaacc gccgcctcaa cgaaggcgat caggtacagg ctgtcatcga
     3361 actccaggaa gaatataagg tggtcccgca ggacatcccc ttggatatcg tctatgaaga
     3421 taaggacatc atcgtcgtca ataaggcccg cggcatggtc gtccacccgg ctgcaggcaa
     3481 tcccgacggg accctggtca atgctctgct ctatcactgc cagggcgagt tgtcgggcat
     3541 caacggcgtc atccgcccgg gtatcgtcca tcgcctggac aaggatacgt caggcgtcat
     3601 ggttgccgcc aagaccgatg aagctcataa ggggctggcc gaacagatta aagaacattc
     3661 ggcgcaccgg acgtactggg ccctggtcca cggcaatatt tcggaagaac ggggcgtcgt
     3721 cgatgcccct atcggccgcc atcccaaaga ccgggtcaag atggccgtca cctttaaggg
     3781 tggccgcgaa gctgtgaccc atttcaaagt attgaagcgc tatggcgatt acacctgggt
     3841 cgaatgtaag ctggaaacgg gccggactca tcagatccgc gtccacatgg cttatatcca
     3901 ccacccggtc gtcaacgacc ctttatatgg gtataagaag gacgatttcc ctatcgacgg
     3961 tcaggccctt cactcgcata gtctcgatct ggtccatccg attacgggga aggccatgca
     4021 ttttgaagcg ccagcgccgg ctgatttcct ggcctgcctg gaacgggcgg aaaagaaaga
     4081 ctgacaggtg gtgagtcaca tgtggaaaga aaagacactg ctcatggacg aaaaagcaat
     4141 cggccgggct atccgccgca ttgcccatga aatcattgaa cgcaataaag gcctggacaa
     4201 gacggtactc gtcggtatcc atacgcgcgg cgtccccctg gctcagcgcc tgggccggga
     4261 actggcagct atcgaaggcc gggatgtgcc cgtgtatgat ctcgatattt cggcttatcg
     4321 cgacgacgac cgcaagagcc ccatcgccgt cagcgatgcc ggcaaattcg atgccgacgg
     4381 ccagacggtc atcttagtcg acgacgtcct gttcacggga cggacggtcc ggtcagccct
     4441 caatgccatc atggaactgg ggcggcctca gttcatcgaa ctggctgtcc tcgtcgaccg
     4501 gggtcaccgg gaactgccga tccgtgccga ttatgtcggc aaaaatgtgc cgacgtcgcg
     4561 gaaggaagac gtgtcggttc aattaaagga aaccgattcg tgcgaccgcg tcgtcttgcg
     4621 ggaacagaat tgattttccc cttatttctt gctatactta agtgaacatc gcacgaagtt
     4681 ttcgaccatc ttatctggtt atcctggcgt taatgtttga agattgatgt aaatttttaa
     4741 ccatccgcta acaactgact gctagccact aaccacttaa aagagcttgt cgcacgtgcg
     4801 acaagctctt ttaactatat cctagaaaga aggggttatc atgaaaaaaa ttgtcaatga
     4861 tgtcaacaac gttgaagagg aaatgattca gggcctggtc aaatcggcac cgaagaagcg
     4921 gcgcaaactc gagtatgggt ccatcgtcgt gcgggccgac aaaaaagaag acaaagtcgc
     4981 cctggtcagc ggcggcggca gcggccatga accggctcat gccggctacg tcggcacggg
     5041 catgctcgac tgcgccgtgg ccgggacggt cttcacgtcg ccgacaccgg accaggtcct
     5101 ggaaggcatc aaagccgtag cgacggataa aggcgtcctc tgcgtcgtca agaactatac
     5161 aggtgatgtc atgaactttg aaatggccat cgaaatggca gccgatgaag atatcaaggc
     5221 cgactatgtc gtcgtcaatg atgacgtcgc cgtccgggac agcctgtata cgacgggccg
     5281 ccgcggcgta gccggtacga ttttcgtcca taagatcgcc ggtgccaaag ctaaacaggg
     5341 cgctgaactg ccggaagtca aggccgtcgc cgaaaaggtc atcgccaacg tccgctccat
     5401 gggcatggcc atcgaaccgt gcactgttcc ggctgcggga aaaccgggct ttgaactggc
     5461 cgatgatgaa atggaaatag gtatcggcat ccatggcgaa ccggggacgc atcgggaaaa
     5521 aatgcgtccg gccaatgaaa tcgccaagat gctcgtcgac cagattctgg cggatctcga
     5581 ttataaaggt catgaagtcg tcgtcctcat caacggtatg ggcgggacgc cgctcatgga
     5641 actgtacatc gtcaacaact tcgtacagga ttatctcaag gaacagggca tcagcgtcta
     5701 cgacacgatg gtcggcaatt atatgacatc catcgaaatg gctggtttct ccatcactct
     5761 cctgcgcctg gatgacgaaa tgaaggctct ctacgatgca ccggccgata cgctggcctg
     5821 gaaaaaataa gagaggatga gttcattgga tacgaaacag atggcagcca tcgtcgaagg
     5881 gatggctaag aaaatcgaag cagaaaaaga atatttgacc gagctcgaca acgaaatcgg
     5941 tgacggcgac cacggcatca acctggcccg cggctttgaa gccgtcgaaa agaagctgcc
     6001 ttccctggca ggcggtgaca tcggtgccct gctcaaaggc gtcggcatgc agctcgtgtc
     6061 gacagttggc ggcgcatcgg gcccgctcta cgggacggcc tttatgaaag ccggcatggc
     6121 ctgcaagggc ctgacggaaa tcgacggacc tgctttcgtc aaagccatgg aagccgctgt
     6181 cgacggcatc aagatgcgcg gcaaggcgac agaaggcgaa aagaccatgc tcgatgcctt
     6241 gtgcccggca ctgaaagtcc tgcaggatga cgtaacggcc ggcaaatcat tgaaagaagc
     6301 cttgcaggat gcagctcaag ctgcggaaaa aggcgtagaa tacacgaaga ccattatcgc
     6361 taccaaaggg cgtgccagct atttaggcga acgcagcctg ggccatcagg atccgggcgc
     6421 tacgtcgtcg ctctatttgc tgcaggtcct ggctgaaatg gcctgatctt taggagtgaa
     6481 ggaaaagatg gtaggaatcg tattgatttc ccacagccag aaggccgctg aaggggccgt
     6541 tgaactggcc cgcatgatgg cgcctgatgc gcctattgcc gctgccggcg gactcgaaga
     6601 cggcagcctg ggaccgagtt tcgagaaaat cacggcggct gtcgaagccg tagatcaagg
     6661 cgatggcgtc gcctgcatca tggatatggg cagtgccgtc atgacggcag aaatggtcgc
     6721 tgaaagcctg gacgaccggc agatacagct cctggactgc cccttcgtcg aaggggcagt
     6781 catcgccgcc attgaagcag ttggcggtac gcctctggct gccatgcagg ataaagtaga
     6841 agccagccgg gacaggaagc tgtaaggtat catgatgaag cgcttcttga aacgcctgct
     6901 cctgttcctg gtctgcctgg ccgtcatctt gggcggtatc ggggcttata tcggaaacat
     6961 ggcttatgaa gaattttatg atgctccctg ggaccagctc ctgggcatcg agaaccacag
     7021 ccgagacgtg tccaccgtcc gccagtggga aaaggaacgg ggctgggaac cggtccgggt
     7081 caatggtcag gacgggacga tcctgcgcgg gacgtatatc gagaaccgtc atgattccca
     7141 taagacggtc atcctcatcc acggcctcta ccagaatcgg tccatgtgcc tgccttatat
     7201 ggaagtctat cgccgcctgg gctataatat cctgctcatc gacctgcggg gccacggcga
     7261 aagcgaaggg gcccatacgg aatggggcat ccgggaaatc gatgacctgg ccatgtggtg
     7321 ccagtggctg cgcaaccgcg atgaacagat gagcatcggc ctgcacggcg tctccctcgg
     7381 agcggccatg gccctgctgt atgcaggcag tgaatacggc aaggacctgt cctttgtcgt
     7441 cgccgatagc tcttatggca atatcatctc cctgggacgg gagaaattgt ggcaggcttc
     7501 aggagataag cgggctatct ggagttatga cctcctggac cccttcttcc aggctgccat
     7561 gttctaccat acccacaaga ccgtgtcttc cctggaaccg gcgcaggctg tgaagcggat
     7621 gaagatgcct gtcctcttcc ttcacggcag cgacgatgcc ctggtgccgg tcaagacggc
     7681 aaagagcctc tatgataatt gtacgagccc gaaaaaagaa ctctatattt ttgccgactc
     7741 tcctcatgct gtcggcattg aaacagaccg cagcgattac ctgcgcgtcg tcagccgttt
     7801 catagaaaag gatgtcctca atgactagga aggcgtcgga ctatgcattt ccgatacttg
     7861 actatttccc gccatcctgc tattattagt atagtaaatg accgccgtct actacatgta
     7921 gtagacggcg gtcatgcgtt tgctatgcgc agtggaggag gaagattatg aacgaacaag
     7981 aatggctcgg tgcagagaat cagctcggcc tggatatttg gaagaataaa tattgcgata
     8041 acggcgaaac ctttgaccat tgggtcggcc gcatcagtgg cggcgatgaa gaagtcgccg
     8101 atttgatcaa aaccaagaaa tttctctttg gcggccgcat cctggccaac cggggactgg
     8161 aatatgaagg caagaagatt accttgtcca actgttacgt catgacgccg ccggaagaca
     8221 gcatcgaaag catcttcgac cgggccggga aattggcccg gccctacagc tacggcggtg
     8281 gccgcggcat cgatatttcc cgcttgagtc cgaaaggggc gcgcctcaac aacgcggcca
     8341 aagaaacgag cggttctatt tcctttatga ccctctattc cctggtcacg gaactgatcg
     8401 gccagaacgg ccgccgcggg gccttgatga tttccatcga ctgtgcccat cctgatgtcc
     8461 tggatttcat caagctcaag acggacctca ataaggtgac taaggccaat atttccatcc
     8521 gcctgagcaa tgaattcatg gaagccgtca aggaacacaa acagttccgc ctccatttca
     8581 agcgacagga aacggggcag gtcattgaaa gcctggtcaa tgcagccgac gtcttccgcc
     8641 tggtcgcaga aacgaactgg gactatgccg aaccgggcgc cctgttctgg gaccgcatca
     8701 aatcgtggaa cctcctggcc cacacgccgg aattttcctt tgccggcgtc aatccctgcg
     8761 ccgaagaacc gttgccggcc ggtggctcct gtctcttggg cagcatgaac ctgtccaact
     8821 tcgtcaacga tccctttacg ccgaacgcta cctttgattt cgatgaattc aagcgctgcg
     8881 tccgcattgc cgtccgggcc ctgaacgatg tcctcgaaga aggcctgccc ctgcatccct
     8941 tggaagaaca gcgggaaagc gtcaaagcct ggcgccagat cggcctgggc atcatgggcc
     9001 tcggcgacat gctcatccag atgggcctga cttatggcag cacagacgcc gtcgccttct
     9061 gtcatgatat cggctttgcc atggccgata cggctatcgc tgcgtcggca gaactggctc
     9121 atcagcgcgg tgccttcgaa aaatgccata tcgatgaaat catgagtacg ccttatttca
     9181 aagccaacac gacggaagcg acggccaaac tggtcaagga atacggcctg cgcaattccc
     9241 agctcctgac catcgccccg acggggactt tgtcgacgat gctcggcatt tccggcggca
     9301 tcgaaccgat ttatgcgaac ttctatgaac gcaagacgga atccctccac gggacggacg
     9361 tctattataa ggtctatacg cctattgtcg aacgctatat gaagaagtac cacatcaaag
     9421 acgacggcca gcttccggaa tacttcgtca cggccctgac cctggattat catgaacgcg
     9481 tcgaaatgca ggcagcctgg cagcagcaca tcgatgcgtc catcagttcg acagtcaacg
     9541 tgcccaacag ctttaccgtc gaagatacgg aagacctcta cctcttggcc tatgaaaagg
     9601 gctgtaaggg cctgaccctg ttccgcgacg gctgcaagcg ggccggtatc ctgacgaccc
     9661 atgaagaaga aaaacgtgta cctacggctg gtgaaggcct ggaacgcggt gaaatcgtca
     9721 gcatcgacga caacgtcatc ggcaagaaac gtaaactcat cacgggttgc ggcagcctgc
     9781 actgcatggc tttcttcgat ccccagacag gggctctcct ggaaacatac ctcagcaagg
     9841 gctcgacagg cggctgcaac aactttatga tcggcttgtc gcgcatgatt tccatcagtg
     9901 cccgcgccgg catcgatatc tataccatcg tcgaccagct caattcgaca ggcaactgcc
     9961 cgtcctattc ggtgcgccgg gcgacgcgcg gcgataccag ccgcggttcg tgctgcccca
    10021 tgacggtcgg caacgccctg ctggaaatgt tcgatgaagt ccaggaagaa ctgcatcaga
    10081 cggcctccgt cgccagcggc cctacgccag tcaaacagcc gccggtccgg ccgaaagctg
    10141 aaaagaaggt cacagccgaa agcatgctct gcccccagtg cggcgaaccc ttagtctttg
    10201 aaggcggctg caacacctgc aaaaactgcg gctggagtaa atgtaattaa gatgctgacg
    10261 ccccatatgg ggaggcccgt cttaccgcat cttgacgcat ctgcctgcgt cagcaattcc
    10321 tcgacgtagc cctgctacgc cttgcggatt gcttccttgt cagatacgcc aatctgcggc
    10381 aatccgaacc acctcatatg gggtgtcagc atcgagtcac ggcctttgtt aggcaatgtg
    10441 gtaaactgac gtttcccaga gacggtatca gactccgggc tgtgggagat ttggaaaagc
    10501 caatgagtcg tgccactggc tgacgctggc ctggcaatcc aactgccccc cagagacggc
    10561 atcagactct atgcaatctc tggaaaattg ggaaaagcca atgagttgtg ccactggctg
    10621 acgctggctt ttaggaaatg ggcaatccgg actgtagggg tcgccacgtg ggcgacccgc
    10681 tggagactga tagtgatagc ccatggcagg atgatgggac tgatttatcg gctacgagca
    10741 cgcacctacg gctgttgggg gatgatcctg ttaaaatttg tcttgacagg cagggcgttt
    10801 gtgtttataa taaacgtaat tttacagaag gcgatgaagg agaagcaaat tatgggtcct
    10861 ttcagagaac tgccgggtgg tgcgaggcag cagggctgta aggcgttccg ctctggagct
    10921 gcaaatgatg aatttgacgg gtacgcccga tatagcggta caagttggcc gaaaggcaag
    10981 ttgggtggca acgcgggaat atcgactctc gtccctttga tgggacggga gtttttttat
    11041 tggtatggca gaagactttc cctgcggtct tcgcgcagta agaggtggaa atatgaagag
    11101 agtgtacaat ttcaatgcag gtccatcggc aatgcctttg gaagtcctca aggaagttca
    11161 ggatgaattc ctcgatttca atcacacggg gatgagcatc gtcgaaatca gtcatcgcag
    11221 tgctgcctat caggaaatgc aggacgacac ggtagcgacg ctgaagaagc tcctggctgt
    11281 tccagatgat tatcatatcg tcttcatcca gggcggcggc agtatgcagt tcgtcatgca
    11341 tgcagcaaat ttcctcaaga agcgcggcgg ttatctgaat actggggtat ggtccaataa
    11401 ggccatggaa gccgcttcct tctacgggga aacgtatgaa gtcgcttcga gcaaagctga
    11461 tggctttacg tacatcccca aggcagaccg tttcgtcgtc aagcccgaca cggactatat
    11521 ttacatgaca tcgaacaata ccatccatgg gacggaatgg catgatttcc cgtctttcga
    11581 cgttcccttg ttctgtgata tgtccagcga tttcctcagc cgtccggtcg atgtgaagaa
    11641 tttcgatttc atctatgccg gtgtccagaa gaatgtcggc ccggccggcg tcgtcgtggc
    11701 catcatcaag gacgacttgc tgaagaaggt ctgccgtgat ttgccgctca tgctgcagta
    11761 caagacctat gtcgaccacg attcgacgta caatacgccg ccggtcttca acatttattt
    11821 cgtcaataaa gtggcccact ggctcgacga taacggcgga ctggctgcca tgcatgaacg
    11881 aaataagaag aaagcggcta tcgtctacgg tgtcatcgac gacagcaacg gcttctaccg
    11941 cggccatgcc cgcgtagatt cgcgcagcct catgaatgtc accttcaacc tgccgacgaa
    12001 ggaactggaa gcccagttcg tcgctgaagc ggctgatcac gacctcatcg gtgtcaaggg
    12061 gcaccgttcc atcggcggct gccgggcatc gatttacaat gccgttaccg aagaaggctg
    12121 ccaggctctg gccgatttca tgaaagcgtt ccagaaaaaa cattaatgta ttacataggg
    12181 ggagaagaaa atgaagattt tagtcagcga tgacgtatcg atgaaaggcg tagatctctt
    12241 acgcgaaaat ggatatgaag tagatgtgaa gaagaacctc gatgaacagg gcctcatcga
    12301 ctgcatcggc gaatacgacg gcctcattac ccgttccatg acccacgtca cggaaaaggt
    12361 catcgaagcg gctacgaaat tgaaggtcat cggccgtgcc ggtgtcggcg tcgacagcat
    12421 cgacttgaag agtgcgacgg tacggggcat cgtcgtcgtc aatacgccga cgtccaacac
    12481 cctggccgct acggaacata cctgcgccat gatcatggcc attacccgcc acatcccgca
    12541 ggcccacaat tcgctcatgg ccggcaactg ggaccgcaag acctttacgg gcatccagct
    12601 ccagaacaag accatcggca tcatcggcgt cggccgcatc ggttcgcgca ttgccaaacg
    12661 catgcaggct atggaaatga agaccatcgg ctatgacccg tacatcccgg aagaacggtt
    12721 caaacagctc ggcgtcgaac aagtcgactt ggatacgctc ttcaagcggt cggactacat
    12781 caccatgcat acgccgctga ccaaagaaac gcggggcctc atcggcaagg aagaaatcgc
    12841 caagatgaaa gacggcgtcc gggttatcaa cgtatcccgc ggggcggtcc tcgatatcta
    12901 tgcactggct gaagccctca agagcggcca cgtcgccggc gccgctatcg acgtcttccc
    12961 gacggaaccg ctgaccaagg acatcaaccc cttcctgggc atggataacg tcgtcattac
    13021 gccgcacctc ggcgcttcga ctgtcgaagc tcagatcggc gtttccgtcg acgtcgccga
    13081 aggcgtcatg gaagccctga aaggcgaacc cgtcgctaca gccgtcaaca tggctcctgt
    13141 accgcccaat gtctacaatg tcatccatcc ttatttcgac ctcatggaac gcatgggcaa
    13201 cctggccgtt tacctggccg atgcgccgat gaaggaaatc gaagtagaat acacgggcga
    13261 acttgccgat accgatacgc gcctgctgac gacggccgtc caccaaggag ccctcaaacc
    13321 gctcctccag gaatcgggca actacgtcaa tgccgccgaa gtcgccaaga cgcgccacat
    13381 tgccatcaaa gaaatcaaca gccagtccaa tggctatttc gtcaactccg tcactgtccg
    13441 ggtccgcacc aataagggcg aacacgccat cgtcggcgtc ttgttcaacg ggacggaagc
    13501 caaaatcgtc cagatcgatg aataccgcgt cgacttcacg cccgaaggct acctgctcct
    13561 ggtaccgcac gtcgaccagc cgaacatgat cggtcagatt tcgagcatcc tcggccaggc
    13621 tcatatcaac atcatgggca tgcaggtcgg ccgcatgacc aaatcgggca acaacatcat
    13681 ggccattgcc gtccaggatg acatccccaa cgacatcatg gtcaagctcc gctcgatcaa
    13741 cggcatcctc gatatgaagc tcattcactg cgatattcac taacaggggg aaactatggt
    13801 acgcatcata ctcattcgcc acggcgaaac acaatggaat attgaaggcc gttaccaggg
    13861 ccaggaagac acgcacctga gtgaacgggg cctccgccag ggccatatgg tcgccgaagg
    13921 cctgaaggat acgcccatcg acgtagccat ttccagcccc ctcgaacggt cgtacatgac
    13981 ctgctctttc tgtgcggacc tccacggcct gccggtcctc aaggacgaca ggctcctgga
    14041 aatcaaccac ggcgattggg aagggaagct ggccgatgaa atcgaagcca agtatcctga
    14101 agaattccac cgctggcata cacagcctca tacggtgacc atgcccggcc agggcggcga
    14161 atccctggaa gatgtccgcg tccgcgcccg cgctgccttt gatgattacg tccggaagta
    14221 cgacggcaag acggtcctcg tcgccgctca tgacgccgtc aacaaggcca tcatctgcga
    14281 tgtcctgggc ctggatatga gccatttctg gcagattaaa caggataata cgtgtatcaa
    14341 cgtcttggaa tgtcaggacg gcgtatggcg cctggtcctg ctcaattcta cgaatcatat
    14401 ggggtatttg ttcagcggta ttgaacagaa aggcttataa ggatataata cgaggggtat
    14461 aaaagcatat ttactgagga aggaagaaac tatcatgtta gatacgaaat tcatccgcga
    14521 acacgtggaa gaagtcaaag aaaatattaa gaaccgccat gccaaagccg atgtcgacgc
    14581 tttcgtcgcc ttggacaacc agcgccgcga cattcttcag aaagtcgaag ccatgaagaa
    14641 cgaacgcaat acggtcacca aggaaatcag ccagctcaaa cgcaacaaag aaaatgcgga
    14701 cgcccagatc gcagccatga agaagctcgg cgacgacatc gacgttctcg acaaagaagt
    14761 caagaaagtt gaagctgaca tgcgcgctat ccagctcatg atccccaata tgtgccaccc
    14821 ctcggtcccc gttggcaacg acgacagcga caacccggaa gtccgcaaat ggggcgaacc
    14881 gacgaaattc gatttccagc ccctcaatca ttgggatgtc ggtgaaaaac tgggtatcct
    14941 cgattccgaa cgggctgcta aagtcacggg tgcccgcttc tattattaca tcggcgccgg
    15001 cgcctgcctg gaacgcgctg tctacaactt catgctcgac cagcatacag aaaaggacgg
    15061 ctacaccgaa gtcatcccgc cgtacatcgt caaccgcgat tccatgaccg gtacaggcca
    15121 gctgccgaag ttctacgaag atatgtacaa gctcaacgtc gaaggcgaag aaatgtacat
    15181 gatcccgacg gcagaagtac cgctgaccaa ctactaccgc ggtgaaatca tcgacggtgc
    15241 caagctgccg gtctacttca cggccatgac gccgtgcttc cgtgccgaag ccggttcggc
    15301 tggccgcgat acgcggggcc tcatccgtca gcaccagttc cacaaagttg aaatggtcaa
    15361 atacgtcgct ccggaaacga gctatgatga actcgataaa ctgaccaaca acgccgaaga
    15421 tatcctcaag gccctgggcc tgccgtacca tgtcgtctgc ctctgcacgg gcgatatcgg
    15481 cttctccgct gccaagacct tcgacgtcga agtctggttc
//
LOCUS       DAWCYS010000012        15302 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_255_length_15302_cov_11.044599, whole genome shotgun sequence.
ACCESSION   DAWCYS010000012 DAWCYS010000000
VERSION     DAWCYS010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 15302)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15302)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15302
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_255_length_15302_cov_11.044599"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3402 a         4145 c         3874 g         3881 t
ORIGIN      
        1 aaaagatgaa aaatttgaca aaaaaataag ccattttact ggcttcacag cggttctttt
       61 attgcaagtg tcaaactccc gaaccacctc acaactacaa cctaaaccct ttcacaatca
      121 tcacggaaac agccctgcca ggtcctcctt cactagcagg gccgttttcg cacgccataa
      181 taactcttca aaaaaaatca gaaaagttta aaaaaagtgt tgacaactat aggcctcgtg
      241 aatataatat aagagtactt gccactatag ctcagctggc agagcatctc attcgtaatg
      301 agagggtcgt aggttcgaat cctattagtg gctccaaaaa atcaagaggc tgtcgcatat
      361 gcgacagcct ctttttgcgt ttgaagttga aagctatcgc ttcaaacatc atacatccaa
      421 ctgctcctaa atcaatttat tttgtttagc cattttttca atcatggaca atgataattt
      481 gctatacttt gccacttttt ccaaggacat tccatctttc aacaattcga gagcaaattc
      541 caatttacct tcttttttac cttcttccct gcctttcatc aggccatctt cgtatattcc
      601 ttctcctaag ttgcacatga ctgccaaatc cccctctccc tacaaactcc atgctacaaa
      661 ctataaagca aaaagggtct cattttttgc cggaaatcgg ggatgaacgg tatgggggcc
      721 aatcattttt tccatccaga cgacgtcata ccaacggtcg aatttgtagc cgctgttgtg
      781 gaaacgaccg accagggtgt agccaaggcg ttgatggaag ttcaagctgc cgttggtgac
      841 atacgggtcc tgatcatcac ggggtacgac aatgcaggcg ttgatgttga ggattcccat
      901 ggatttcaag gctttttcca gagcttcgta cagtttacgg ccaatcccgt gtttacgggt
      961 gtttacatcg acgtagatac tcgattccac cgaccaggcg taggcttcac ggccatagaa
     1021 tgcatgggca taggcataac cgatgacctt gccgtctgtt tcggcaacca agtagggata
     1081 gtgctgcgaa atctcttggc ggcgtcgttc catttccacc atatccggcg cggtgtattc
     1141 aaacgtaatg gccgtctttt ccacatagtg tgcgtaaatc cgggccaaag ctgccgcatc
     1201 actttcacag gctgaccgaa tcgtaatatt atcgctcatg gccgtatctc tccttttcaa
     1261 agaacctctc agactgcctt cggcagccag ctcccctatc atgggagcct taacctctca
     1321 gaccgccttt ggcggccagc ttccctatca tgggagccta aaaaaagact atggcatgct
     1381 ctcaggattt ccatgccata gtccctgttc ccggtatatg attatttcca gaattcccac
     1441 caatggcggt tgtctttttc ggccagctgc tggcgcaggc tgtctcgttc ggcctggacg
     1501 gtggccagtg tttcctggag ttgtttgtgt tcgtcttcga tgatactgcc ttcttttttc
     1561 aggcggtcca gttcgctctg cagggcatcg gcctggtcct tgttctgctg ggcttccagg
     1621 gctttgactt tgacttcaga cagttcgttc tgtttggcga tcaggttctg ctgcagcgtg
     1681 atgacctgct gctgggccgt gttgaggtcc tgctgggctt tgagcagcaa aatacgcagg
     1741 ttgtgcgatt cctgctgggc attggatact ttttcgcttt ccagttcggt aatggtcgcc
     1801 tggtcgtgga ggccgcgcag ctggtcgagg atgcggacag cttccgtatc gaaggcccat
     1861 tcgcccttct gcatggtggc gtgttcttct ccgtcatgat tgatttcgtc cagatgtttt
     1921 ttgacgaact ggatgatgcc ctgacgggtc ttgatattat agcggtccat taaatctctt
     1981 gtcgtaatca cgtgttttct ctcctcatgt ttactatctc tgtttttgca tttacctggc
     2041 tcattttccc aataaaaaaa aggcttaacc atgccagcca gcgcaacagc aattcatata
     2101 aatcaattac ctttagttta ccgtatttca aagaataatg caacgaaaag ggggctgtca
     2161 catgaagatt cccgtcatgt gaggccccat ttcagtcgat gtgcaattag tatctatccg
     2221 attagatgga aagctggacg aaattctggc caaagcgcag gtattcgcct tggggcagtt
     2281 tataggcttt ggaaatggcg ttggcttctg tcgggacttc cggcgtcgtt tccaattcgt
     2341 aagatttggc ataggcacct gctgtatcag aagcggtcag gccagattta tcgaacttca
     2401 aatcgctgcc gtcataaatg cggataccga tgacaatgcc gccctggaga taagcatcca
     2461 tgtgatggtt atcataaatc cagcggtctg ttccctgatc ttcggcagta ctgctcggct
     2521 ggccataggc agccgtcagg tcttcctggg tcatgccgag gtagacgccg ccaatggatt
     2581 cgtaacggtc ggcgttgagg gaacggcgta agggcatctt attgtctacg atgaggtact
     2641 gatgggattt atggcccatg aggtccatct tgatcgtccg gtcgccagtc tgttcatgga
     2701 tggtgaaggt cccggtccgc ggatagtcat aggtgcagtc tttcgggtcc gttaccgtac
     2761 agccgttgat ggtgttgcct tcgatggtca tgaccaggtt gcccttggtg ctgtaccatt
     2821 caccggcagc cgggtcgagc cagtcgacga cattctggtc cggagcggcc tgagtcgttg
     2881 tcgtcaccgt agctgttgcc tgcggttctg ttgccgtcgt ttccgtcgta gtcgtatctt
     2941 ctgcaaagca gacggctgat aaactcatcg tcacgagcgc acaaaggatc atattttttg
     3001 ctaatttttt cattctcatt cgctcctctt ctgtttactt tctgtaactg ttaattatac
     3061 acatcttcca gaaagaaaac aaccgtcttt acttctgttt atgtttgttt acacttttct
     3121 tagcatgttg cttagcactc tgcttatctt tcttggtctt cgtttccggc gcctttacgg
     3181 tgttgtctgc gtttttggaa acttccgctt cctggtgctt ggccgggatg gaaacggtat
     3241 cgatcagggt gccgtcattt ttcatggcgc tgacgcggaa ttcatccggt tcgacctgga
     3301 gcgtcaggta catgggcata tccattggat tatagtaaat gacatcagtc ggcttcttga
     3361 ccgagccatc ccaggcttct gtcccggagc ggcccgtggc aatgtagacg gtcccttccg
     3421 tagcggcctg gtcgttcttg atgggaacgg tccgttcata gcagtgttca tgagccgtaa
     3481 ataccagggg gacgtcgtat ttatcgaaga gcggcatgaa gtatttgccg tttacatcga
     3541 gggtgccgtt atacgggctg tcccaaggcg gacggtgcat gaggacgacc agccgtttcc
     3601 cggccttctt ggccgcagcc aaatccttgt cgagccaggc tgcttcatct tccatcatcg
     3661 tcggacgcca gccgttcaat tcttcataat ccgtattgag ggacacgtag tggacgtcgc
     3721 cataatcgaa ggagtaagcc aggcggttct ggtccttcgg tccaccatag ggcacggcga
     3781 acagggcctt atacgtatac ggttcggcaa atttccaatc catgctatag gcttcgtggt
     3841 tgcccaggac cggggcaaag gggatgtggc tggtcaggac atcggcattt tcaaaccagt
     3901 cccgccattg gaagtaggcc tgtccgttgt cggtaatgtc gcccatgttg atgaagaaag
     3961 ccgcatcatc gttctgctgc caggcgatct gcgccgtctg cccccagacg ctgtaatcgg
     4021 tggactgaga gtcgccgaag acgaggactt tgtatttgtt taaatcttct tttgtcgtcg
     4081 aaaaagtaat ccaatcggtc gcaccgtcag cattgacgat gcggtattca tattccgtcg
     4141 ccggtgtcag tttctgcatg taagccccat acgtataggg tgccggattg tcggcgtcgt
     4201 aaatgggagg ccgtttccct tcggtcacat aagccaactg gacatcaccc tggtccttgt
     4261 gacggtattc tacactgtaa tcgctacggt cgctgtcata ctgccaggaa atggtcttgc
     4321 ctatcgcact gtccttggcg acgacctgca tcaggttatc cacggacatg gccatggcct
     4381 gaccggaaaa taaagtaaac gcactgagga gcgccgccag gagatggcgc tttctatatt
     4441 tcatgtgttt tcacgtcctt tcttgtcaca aaatgtattg tagcacaatt cgttgatttg
     4501 gcaatctttt tcgttgacat cgatttccct atctcctata ataattgaat tgtatattgc
     4561 tgcattgatg aggagagaag agaatgaata cacttgattt actcttgctg gccgtcagcc
     4621 tatccatgga tgcctttgcc gtttccatct gtaaaggcct gtcgctggga aaaatcaact
     4681 ggcgccacat ggtcctggcc ggcctgtggt ttggcggctt ccaggctctg atgcccacta
     4741 tcggctatgg tctgggcagc tttttcgccg acatgatcga tgcctatgat cactgggtca
     4801 cgttcatcct gctcagcgcc atcggcctca atatgattaa ggagtccttt gaggacgaag
     4861 agcactgtga tgcgtccatg agtccctata cgatgctcat gctggctatc gcaaccagta
     4921 tcgacgccct ggccgtcggg gtcaccttcg cctttatgga catcgccctc gtgtcgtctg
     4981 tgacgctcat cggcgtgacg acgttcctgt tctcggcagc cgggataaaa gtcggcagcc
     5041 tcttcgggat gcgctataaa aataaggccg aacgcatcgg cggggccgtc ctcatcttga
     5101 ttggtttaaa aatatttctg gaaggcttgg gcgtgttgta aaaaaaaggc ttgtcgcaca
     5161 tgcgacaagc cttttttctg taggggcgcc acgtggggcg cccgttcgcg caaatcggtt
     5221 cacgggcatc ttacatatac ccgcgggctc gccacgtgcg agcccctacg aaatataaat
     5281 tatttaactt cgtggatcca gccttccggt ccttcgattt cgcccatctg gatgccccgc
     5341 aaggtcttgt acagtttcgt caggacagga cccatttcgt ccatgccgct ggggaggcag
     5401 atttctttgc cgtggtcaac gactttgcct accgggcaga tgacggcagc cgtaccgcag
     5461 aggccggctt cagcgaattc gctgacttct gcgaacttaa ccggacggtg ttcgactttc
     5521 atgccaagca tgtccattgc gatcgtgatc agggaacggc gggtaatgga cggcaagatg
     5581 gagtccgatt tcggcgtaac caaagtgcca tctttcttga cgaagaggaa gttagcgccg
     5641 cccgtttctt ctacgtacgt gcgcgtagcc gggtcgagga acatgttttc atcgaagccg
     5701 ttctgatgag ccaaaatgta aggatacatg ctcatggcat agttcaagcc ggctttgata
     5761 tggcctgtgc cgcgcggtgc tgcacggtcg aagtcagaaa tgcagatcgt aatcggtttg
     5821 acgccgttct tgaagtaaga gccgacaggc gtgccgaaga ggcggaactg atattcgtca
     5881 gccggtttga cgccgatgac cgggttagtc ccaaagagat acggacgcag gtagagagcg
     5941 ccgccgcttt caaagggagg aatataatcc ttattggccc ggacgacttc gtcgacagct
     6001 tccatgaaca tatcttccgg aaccggcggc atttccaagc gttttgccga gtccatcaaa
     6061 cgttttgcgt tctggtccgg acggaaggtg accgtgcggc cgtctttggt tttataggct
     6121 tttaagcctt caaagacttg ctggcagtat tgcaggatgc cggcacattc attgaggacg
     6181 acattcgggt tatccgtcaa ttcccctttg ccccatttgc catctttata gttggcaaca
     6241 taacgttttg caatcggctg atattcaaaa cctaaggccg accagtcaat attaacattt
     6301 gacatcgcaa ttcctccatt tcttttttaa acaaaataat ttatatctat tttgtaatta
     6361 tagattaatc acttctttcg cttttgtcaa tgggagaaat tctcaccgga ttattgttaa
     6421 ctaatttgta ctatttaaaa attgacaata agtcgattat tggctataat ccagattatc
     6481 gtaaactttt taaatattat tgtggtttac atttatccaa aggagtgatt tcatggtttc
     6541 ttctgtcctc tcagcccacc actgggatac gcgggatatg gtcttattcg ccctgttcac
     6601 ggcgctcatc gccatcgggg cctttatccg catccctgtt ccgttctgtc cttttacgct
     6661 gcaattattg ttcaccaccc tggccggact gctcctgggc agccgccgcg gcgccgcctc
     6721 ggtggccgtc tacgtcctct tagggctggt cggactgccg gtcttcacgt ctggcggcgg
     6781 tccgtcctat attttccagc cgaccttcgg ctatctcatc ggcttcatcg gcggtgcctg
     6841 gctgaccgga tacattgccc atcaaagcgc catgccatcg ctgcagcgcc tgctggctgc
     6901 tgattttgcc ggcctcatca tcgtctacgc ctgcggcatg gtctatttag ctttaatcaa
     6961 taacgtatac ctgggcacac cgatcagtct gtggacactc ttcgtctact gcttcgtcct
     7021 gcccattccc ggtgacatct ttctctgcat cgtcgccgcc ttcctgaccc gccgaatacg
     7081 tcggaccact gccctctaga aaggagttcg tttactatga gtaaaggttt attcatcacc
     7141 gggacgggga cggacatcgg caagacctat gtcacagccc tgctcatcaa gacactgcgc
     7201 cagcacggtt atgacgtcgg ttattataag gctgccatca gcggcgcccc gaccgtcgcg
     7261 gccagtgatg ccggttatgt aaatgagata gccgggattg gcgaatcgcc ggacctgctc
     7321 ttgtcttatc tttacgacca cgccgtgtcg cctcacctgg cagcccgctg ggaagagaag
     7381 cccatcgaaa aaagcgtcat cctccgcgac tggcagcgcg tcacggcagc ctatccctac
     7441 gtcacggtcg aaggcagcgg tggcatcatc tgtcccctcc gcgaagatga tgccatcagc
     7501 ttccagctgg aagatctcat cggctggctg cacatcccat cgctcatcgt ctgcccggct
     7561 ggcctgggga ccatcaacgg cgccgtcctg accgcctttt atatgaaaga aaagggcctg
     7621 cctgtccaag gtttcatcgt caaccattat acgggaagca ccatggaaaa agacaacatc
     7681 cgcatgattg aaaagctcag cggcaagccc gtcctggccg tcgtcggcga caacgccgaa
     7741 cttttacagg ccgatgccca ggctttgatt caactatacc agtaaggagt gatttacatg
     7801 agtttacttg aagctgactt aaaatacatc tggcatccgg ctcagcagat gaaagacgcc
     7861 gaagtcttcc cgcccgtcgt catcgaccac ggcaaaggct gctatctcta tgacacagaa
     7921 ggcaaagaat acatcgacat catcagttcc tggtggtgca atctcctcgg ccactgcaac
     7981 gacgaaatca atgacgccat caaagaccag ctcgaccagc tggaacacgt catctttgcc
     8041 aatttctccc atcgcccggc catcgagctc tgtcaggcgt tgctgccgct gctgccaaaa
     8101 gggctgacgc atttcaattt caccgacaat ggctcatcgt ctgtcgaatg tgccctgaag
     8161 atggcctttc aatatcagta tcagtcgggc caccctgaac ggcagcgttt catgtgcctg
     8221 tctgaatcgt atcacggcga gaccatcggc gccctgtccg tcggcagtat ggacctctat
     8281 gcccgcattt acaagcccat gatgatgaat aacatccatt tcgacggcct cgactgctac
     8341 cgctgtccct atggccaaac gcgggatacg tgtgactgcg aatgtttcgt cggcgctgaa
     8401 aaggccttcg ccgcttacgg caaagaaacg gcagccctca tcgtcgaacc gatcctgcaa
     8461 ggcgcggcgg gtatgcgcat ctacccgcca gaatacctgc gccgcctgcg ccagctctgt
     8521 gaccagtacg gcgtcctgct catcgacgat gaagtcgctg ccggcttcgg ccggacgggc
     8581 aagatgttcg ccattgaaca cgccggcgtc agcccggata tcctctgcac gtcgaaaggc
     8641 ctgaccggcg gctacctgcc catgtccctg acgattacga ccgatgccat ttaccaggcc
     8701 ttctacgacg atttcaagac ccataaggct ttcgtccaca gccacaccta tgccggcaat
     8761 cccctggcct gccgggcggc cctggctgtg ctacacatca tggaacgcga cgatatatta
     8821 aaaaaagctg ccgaaaagag catctacctt cacgaaaagt tagacgctgc tctcggcagc
     8881 cacagccatg tcggcgaaat ccgccacatc ggcctgatca atgcgattga attagttgaa
     8941 gataaacgga gtaaacgcgc ctttgacccg gaaaagcggg tcggctggca catcttccgc
     9001 aaagccatgg acctgggact ggtcctgcgc cccattggcg acgtcattta cttcaatccg
     9061 cccctcacca ttgatgagca gaccatggat gaagccgtcc gccgctgccg ggatgccgtc
     9121 catgccgtct taggtggcga ttaagccttc agcgccgcat ccagggccgc tttgacatgg
     9181 gccttgatgt cgtcataaga ctggcctggc tgggcttcgt agagtttttc cgtgccgatg
     9241 aacatagacg gcacatagta gtaagaatgg ccctgtacga cttcggggtg ttccgattct
     9301 tcgtaccagt cgacggctac ctggcggtaa gccggatttt cttcgatcag ttcagccaag
     9361 gcttctttgg cgcccttgca ataggggcag ccgttcatat aaaaagcggt gatattcttc
     9421 aagggaatcg tctcctttcc agggaacaga caggttactg atgacctccg cagccgtggg
     9481 aaccgcagtg atgttcacca tggccatgct gctggtcgtg atggctgcaa gctacgtcgg
     9541 gattgaattt cagcgtacca ttgagcaaat ccttgacggc ttcatcggca ctgcccgtaa
     9601 cgccgccata gaggtcaatc gaagcggccg ccagggcctg ctgggctccg ccaccgatgc
     9661 cgccggcaat gacggcattg atttcctggg cctgcaggaa accgaccagg gcaccatggc
     9721 cctgaccttt ggtgtcgatg acgtcagcct tgacgatatg cccatcttcg acatcataaa
     9781 tcttgaattt ttccgaatgg ccgaaatgct ggaatacatt cccattttca tacgtaactg
     9841 ctactctcat cataacaatt tctcctccaa tctgcaataa ccagacgggt cctcgcggcc
     9901 cgtctttttt tgacatatgt cgattaattt gattttagta tatgacatat gtcagtattt
     9961 gtcaatgaaa aagaggctgt ctcattaaga cagcctcttt ttgagttcat cgtaggtcgt
    10021 ttatcgttcg tcgtaggggc gcccacgtgg ggcgcccgtt cacgccaatc ggtgcatggc
    10081 catcttgcat atacctgacg ggctaaaaaa gcacgtgtaa gcccctacaa aaaagccatc
    10141 gcatcaaacg tcaatcattc ccgcttcccg gcgagatgac gacggtcggt gtactgttgc
    10201 ctttgacgga gtacatatta tagacatagt ccggcgtcag ttcacctttg agatggaaca
    10261 gggagtaggc gtcgacggcg ttcatgtaaa cgacgtcgct cttgacgtat cccggggcga
    10321 tgccataagg actggtcagg atgccctgct gggaattcca ctgtaaatcc agattcagcg
    10381 ccgtcgccac ggctacggac ggcaggtaaa tctggccatt cttgcccagt gcccgcttgg
    10441 ccagtttatg gccgtttaca actaaatcgt aagccttggc gatgtacgtg acgttgccat
    10501 cggaggcgtt gatcttatcg gagatttcct ggaaatcagc aaaatcttct acgccatagc
    10561 cggccagggc cttcttgacc tgagcgatgc tcagggactg ccagccgtag ccgtcaggat
    10621 agatataata agaaaccgta tggtccgggt cgacgtcgat gaccaggcgg tcatagtaga
    10681 ccgtaaccgg cgtcccgacg gatacatact ggtagaggtc ttctacatcg gcttctttca
    10741 tgcgcacgca gccattggat acatagccgc cgatggattc aggatgattg gtcccatgga
    10801 tcccatagtt accgtaaaag ccgatccagc ggtagccaat agggttgtcc ggacctgaac
    10861 cgatctggat gctcgtgtca tccgggtcga cccaggtcgg gttgacttcc ttatattgga
    10921 cggcatagta accggtcggc gtcggcgtcg acgtcttgcc gaccccgacg tgatacatcc
    10981 tgaccttcgt atcgccttca tagagcgtta agatacggct ggccaggttg atacctattt
    11041 tcttttccga ttcgaccgcc ttggcctgct ggccttcctt ggcggccgtc tggcttttcg
    11101 tgtccttctt cgccgttacg gctggctggg ccggtttcgt ttcttcctgc ggcgccgccg
    11161 tcggcttgat ttccactttg actgcaaaag gagccggtcc agtcttcagt tccggcgtat
    11221 cggcccaggc agctccagcg gtcaaggcca ggacgcaggt cagagccatc attcgtttca
    11281 gcaaaaaaat ccctcacttt catcgcgctg atgcgccttt attttgattt acatttattt
    11341 aatcttcgtt tacatcatct tcccggccgc tgccattcag aatcaggttg aggacgacag
    11401 ccgtaatact gcccagggta atgccactgt gcaggacgat gttcgcccac gacgggaagc
    11461 tgtggaaaaa gttcggcgcc gtcagggtga tcatgctgac gccgatactg acggcgacaa
    11521 tcatcagatt gtacgtcccg tcgaatttaa ctttactcaa ggcccggata ccgctggcaa
    11581 tgaccatgcc gaacatggct acgcctgcac cgcctaagac ggaattcggg atagaggcga
    11641 cgacagccgc catcttcggg aacaggccca aagcgatgag gatgatgcca gacgcggcga
    11701 cgacgaaacg gctcttgacg cgggtgacgg cgatgaggcc gacgttctgg gcaaaggccg
    11761 tataagggaa gctgttgagg ataccgccga taaaggtcga taagccatca gcccggaggc
    11821 agcgtgccag gcggctgcgg ccaatggggc ggccgacgat ggacccgata gcgatgcagt
    11881 cccccgtcgt ttctaccatc gtgacgatca tgacgacgat catcgacagg atcgagccga
    11941 tatcaaaagt cgggaagccg aaggaaaaag gcgtgactaa cccaatccag gccgcgttgc
    12001 cgacttcggc gaagttcgtc tgccctaaaa tcatggcaat gatcgtcccg ccgatgagtc
    12061 ccagcaggac agaaacattg ctcaagaagc ccttgccgcc gcggtaaatg gcgatgatga
    12121 tgaccagggt gatgaaggcc aggaacaaat tcatcggtga ggcgaagtcc ggcgacttcg
    12181 gattgccgcc gcccatccag ttgacggcta ccggcagcag ggtgataccg atgatggtga
    12241 tgatcgtccc cgtgacgaca ggcgggaaaa agcggatgaa ccggctaaag aaaggagcaa
    12301 tgaagaaagt gaacagcccg gcaatgagaa tcgatccgta aatggccgta attccgtgtt
    12361 ccgccccgat gaggatcatc ggcgtgaccg acgcaaaagt gaccccctgg atcatcggga
    12421 tgcgggcgcc gattttccag aagcctaaag tctgtatcag cgtggcgatg ccgcaggaaa
    12481 ataagtcggc attgatcagg tggaccagtt cagccggcga caagtgcagg gtctgggcaa
    12541 tgatgatcgg tacggctaca gccccggcgt acatggccag gacgtgttgc agaccgtagg
    12601 caaagagccg gccagccggc agcatttcgt caacgggatg gatccgttta gtcaggtttt
    12661 cgttcatttc gtaaaagtca cctcatgttc aaaaaaagcc tgcaccatgg caagcgattt
    12721 tttcttaaca atatacatac ctataaaaaa taacaatacc gtttttatta taccattgac
    12781 tcaaaaaaaa taaaacatgt agctaaaatt tactcaatct ttacatattc ttttcaaaac
    12841 aatgatatgt ctctcatata ctgattttaa gtacacagag gaggaaataa catgttcagt
    12901 atcagcaaca agcacgaaga attcttcgat tatctcatga ccaacgccga aaatttccat
    12961 cgcggcgccc tcatcgccca tgaagtcatg gaggacgttt caaccatttc catgcatctc
    13021 aaggaaatcg tcaaactgga acacacgtcg gacaaaacca accaggaagt catcttcaaa
    13081 ttgtcccgtg tcttcatcac gcctatcgac cgggaagatt tttacaaact gacgtgtcag
    13141 ctcgaagact gcatcgattc cctgcaaggc gctctcatgc gcaccagcat gtatcacgtc
    13201 acggaagcgc cgaaagcggc catcgccatg accgaacaga ttgtcactat gggcgaagaa
    13261 ttgaagaaga ttttctcttt cttgaaggat atcgaccaca acgaagccaa actcatgcgt
    13321 catgctgaac gactgaaccg cctcgaatcg gaagtagacc atatctatcg ccaggaaatt
    13381 tccaggctct tcagcggcga tgaaacggac ctgctcaacg tcatccgctg gaaagatatc
    13441 ttagggaccc tggaagaagc ggccgaccac gtcgaaaccc tgggaaatac cattaaagag
    13501 gtgaccatga aatatgcttg atatgtcgct catcatcatg gtcgtcgtcc tggctctggt
    13561 ctttgacttc atcaacggct tccacgatac ggccaatgcc atcgcaacct gcgtatcgac
    13621 acgtgccatc cgccctggcg tcgccatcgt cggtacggct atcctcaact tcctcggcgc
    13681 catgatttcg acaggtgtcg ccaagacgat tggcggcgac atcgtcgtct cgccgacgtt
    13741 agtcaacgaa atgatcatca tcgccggcct ggtcggcgct atcatctgga acctcctgac
    13801 ctggtgggtc ggcatgcctt ccagttcgtc tcacgccctc atcggcggca tgatcggtgc
    13861 catcatcgta tcggccggcg tagccgccct caacggctgg ggtatcctga aaatcgtcct
    13921 gtccctcatc atttcaccgg tttcggccat cattacgggc ttcatcatca tgaagatcat
    13981 ctttgccgtc tgccattcat tcaaaccggc caaggtaaac ctggcggcca acaggctgca
    14041 agtcgtgtcg gcggccctca tggctttttc ccacggctcc aatgatgccc agaaatccat
    14101 gggtatcatc accctggcat tggtatcggg cggcttcatc accagcctgg aagtaccgga
    14161 cctggtcaaa ttcctctgcg ccctgtccat ggccctgggg accagcgtcg gcggctggaa
    14221 gatcatccgt accgtcggcg gcaagatatt caagatgcat cccgtccacg gctttgcagc
    14281 cgacttgaac tcagcggtcg tcatcttctc ggccaccttg ctgcacctgc ccgtctcgac
    14341 gacacacgtc gtttccgggt cgattatggg tgtcggttcg gcacaacgcg tcaaagctgt
    14401 ccactggagc gtcgcccggc agatggtcac ggcctgggtc atgaccattc cctgcacggc
    14461 tgtcatgggc gccttgtcgt acgtcatatt gacaaaagtg ttcttttaaa agacatagta
    14521 aaaggggctg tcgcatgtgc gacagcccct ttttgagttt taggtttgta gtttgtagtg
    14581 aaggttttta aatttaaagc cctcttctac aaactccgta ctacaaacta tagagagaaa
    14641 gggaccacga atgcgggacg ccctttgggc gtcccctacg agccactagc cacgagccac
    14701 taactgctaa cggctgactg ctaacccctt acttcacaat ctcgatctgg tttttgagtt
    14761 cgttttcaaa tttgatgcga tgggctttgg tgtagaggcc aggcatgccg ccggtatgca
    14821 cgaggatgac ctgccggccc agtttgattt tcttttcttt gatcatcgac aggacgccgg
    14881 caaacatctt gcccgtgtag cacgggtcga ggaggatgcc ttcgtgacgg gccatggtgt
    14941 agatggcttc gcggacccgc ggttccggtt cgttgtagcc cttgccgata tagcccgttt
    15001 cgacgtggaa gtcgctgtcg aaatcaaagc catattcttc cttggcctgg ttaaagtagg
    15061 cctttaattt ttccatgtcg aagggcatga cggcaatgcc taccagtttc aaattcgact
    15121 gaatggcttt caagccgcaa tacaagccga gatatgtacc catactgccg acggcgacat
    15181 agaccgtcgc cccgtcgatc tgctgttcct tggcctgggc gtcgagttcg cgggcgcagt
    15241 ccatgtagcc caggattccc gtcctatcgc tgccgcccat ggggctgcag taaacttgtt
    15301 ca
//
LOCUS       DAWCYS010000013        15116 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_260_length_15116_cov_6.914348, whole genome shotgun sequence.
ACCESSION   DAWCYS010000013 DAWCYS010000000
VERSION     DAWCYS010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_15116_cov_6.914348"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3120 a         4024 c         4405 g         3567 t
ORIGIN      
        1 gtctattccc ctttatgagc agattcggca aaataacggc ggatgtgttc caggtcatcc
       61 ggcgtatcga tgccgatgaa ttggtgatga gtctttatca cttttatctt ataaccattt
      121 tcgagaaccc gcaactgttc cagggactcg actttttctg ccggcgtttg ttccatggcc
      181 gcgtactgca ggagaaaatc gcggcgatag gcgtagatac cgatatgctt atacggctgg
      241 atgtcccctt cttcaaagga atggcgcgga tacgggatga gggaacgcga gaaatacatg
      301 gcttcatcct tttggttgag gacgactttg acggcgctcg ggtcatcgta ttcgtcttcc
      361 tgcaaaggcg cagcgaccgt cgccatggtc aggtccggtt ctgcttcaaa ggcccggcac
      421 aagtcgtcga tgacgtccgg tgcgatgagc ggttcgtcgc cctggacgtt gacgatgacg
      481 tcagcatcgg gaaaatggcc gacggcttca gccaaacggt ccgtgccatt ggcgtggtct
      541 tcgcgggtca tgacggcatg gccgccgaag gtgacgaccg tatcatagac tttcgggtta
      601 tctacggcga cgacgacagc cgtcagtttc gaggccttgg cagcctgttc atagacacgc
      661 tgtaccatcg gtttgccggc aatatcgcac agcggcttgc ccggcaggcg cgtcgatgca
      721 tagcgcgatg ggatgatgca tacaaagttc atagtttatc ctccaatact gcttgcaaaa
      781 tcgtatctat ctggtcctgc cctgctgtga attcaatagt aatgctcaag acgtacagcg
      841 gcaggtcgta ttccttgatc agcggcgccg gcagtttgac ggcgtccttt tccgtcgtca
      901 ccaaaaggca gcccgtttcg gcggccctgg cggccatgtg gcggatatcg tcgcggctgt
      961 aatggtgatg gtcttcatag cggagagagc cggcgacggt gaagcccgca tcggccaggg
     1021 tccgttcaaa ggaagccggg tttcctaggg ccgatatggc caggacggac tggccgtcag
     1081 gctgtaaggc cccggagcca tggccctttc cgtcatgcca ggcggcaaag gccaggcacg
     1141 aagacggctt gtggatggcc atggccaccg gcgcggccgg gttgtactgg cgcagcttgg
     1201 cggcgatggc ttcggcccgg tcctgcgtaa tctggtcgcc cttagtgatg atgaaggcat
     1261 cggcccgacc gagctgctcc atgggctccc gcaggatgcc ccgcggcagg acatggccgt
     1321 tgccgaaggg attggtcccg tcgacgagga cgatgtccag gtcacggtac agctgccagt
     1381 gctgaaagcc gtcgtcgagg atgaggacct gtgtaccgaa gcgttcgacg gccaggcggc
     1441 cgccgagggc ccgcttgcgg ccgaccagga cggggatgcc aggcaggctg cgggccatga
     1501 ggcaggcttc atcgccgcct tcttcggccg atagcaggac gcgctgtcca tcggacatga
     1561 cggcagcgcc gccatcttcg cgctgagagc ggtagccccg gttgagcagg gccacgcgcc
     1621 agccctggga ttgcaggcgc cgggccagca tgcacgccat cggcgtcttg cccgtcccgc
     1681 cgacagtgat gttgcccagg ctgatgacga cggccggcgc gtgttcctgc cgttccggat
     1741 ggtcggcgat ggcgtgatac ttgaccagga cgcccttgcg gtagagccag gcggcaccgc
     1801 gcagcagggc cagggccggc cagtcccagc agtgatgggc gccgcttccg gtcaaataat
     1861 ggaaataggc ggcagcccag tttcgcaaag ccatgggctc acctcccgcc gtggacgtcg
     1921 tccagcatct tctgcagctg ttccaggttc ttgcgcgtag ccccctggtt ttcctggacg
     1981 acttcgatgc agctgcgctt catggcttcg gcccggtcgg gatggatgag gatatccagg
     2041 caggtgtcga tgaattcctt ttcattcttg accatgacgc aggcgccgcg gctgctgagg
     2101 agatcgaaga tttcgacgaa gttgaacata ttgggaccga cgacgatggg cttgccatga
     2161 gctgccggtt ccaggatatt atggccgccg atatgggcca ggctgccgcc gacgaagatg
     2221 aggtcgccca ggctgtagac gcgccccagt tcgccgatgg tatcgaggat gacgccgtcg
     2281 tattcggacc ggatcggttt cccggccttc atatcgctac gcttgaccat agtgacgccg
     2341 tgcttgatgc cttcgtcgat gatgagatcg gcctgataga tatagcgcgg ggcgatgatc
     2401 aatttggctt ccgggaaatg gtcccgtatc ttgacgaagg ccttgtagac cgagccgttc
     2461 tcccctttgt gggtactgcc ggcgatcatg atgggatacg tcccttcggc aaagcccagt
     2521 tcgtggagga agcgcttctt ttcggcttcc gtgacgatgc cataggtctg gtcgtacttg
     2581 gtgttgcccg tgacgatgac cttgttggga tcggcgccga tgtcgatgat atactgggcg
     2641 tcgatgcggc tctgcatgca gaacatgcgt atcgtcgaca agacgcggcg cgtaaagaag
     2701 gtaatcatgc ggtaacggac agcactgcgc tggctgatgc ggccgttcat catcatgacc
     2761 ggaatatgct tatctgcggc gatgcgcagg aaattcggcc agatttccgt ttctaccagg
     2821 acgatggcct ggggattgac gatggtcagg atgcggttgg tcagatacgg caggtccagc
     2881 gggaaataga ggatcccgtc ggcgcccttg atgatctgat gggccatgtg gaagcccgtc
     2941 gccgtgacga cagagacgat gatgacttcg tcgggatggg tcttgcgcat ttcccggaca
     3001 atcgggctgg ccgcgacgat ttccccgacc gacgcggcat ggacccagat ggcatggcga
     3061 ttggatattt tatcttttaa ttcttccggc aagaagccga tgctctgttt gatgcgctga
     3121 taaaatcctt cttccctgat gagccggtaa atgagcatgg gaatgagcgt agcccaatag
     3181 atgaggagca ggatattata cagccagtac atggggtgcg attttagatt cttcatcaca
     3241 atcaaccttc tgacgctgtt tcttccgtag tatcgttgaa ctgtacggca tagagatggg
     3301 catagaggcc gtcttttgcc agtagttcat catgggtccc ctcttcgacg atgcggccgt
     3361 ggtcgatgac gacgatgttg tcggcattgc ggaccgtgct caggcggtgg gcgatgacga
     3421 cggccgtgcg gccttccatg aggcggtcca gggcagcctg gacgatcttt tcgctttccg
     3481 tatcgagagc cgacgtcgct tcatcgagga tgaggatgcg cgggttcttg aggatggctc
     3541 gggcaatggc gatgcgctgg cgctggccgc cggacaggga gctgccgcgg tcgccgacga
     3601 gggcttcgta gccgccgggc agttcccgga tgaagtcatg ggcattggcg gctttggcag
     3661 cggcgacgac ttcttcgtcc gtcgcatcga ggtggccgta gaggatgttc tcccggaccg
     3721 tagcgttgaa aagcatcgtt tcctgcggga ccaggccgat ctgctggcgc agggacgaga
     3781 aggtgacgtc gcggacgtca atgccatcga tggtcagact gccgcccgtg acatcgtaga
     3841 aacggggcag caagttggcg accgtcgatt tgccggcacc gctggggccg acgagggcga
     3901 cgacttcgcc aggggcgact ttcaaattga aatcggtaag ggccgggtgg tctttgtcat
     3961 aggcaaagga gacatggttg aattccactt cccctttgac cggcggcaag acgctggcgt
     4021 caggcttttc cttgacatcc cgttcgctgt cgattgtttc aaagacgtgg tcggcagcgg
     4081 ccagggattt ctggatatcg ccgtagattt cactgattcg gcggaccggg ttggccaggt
     4141 tgatggagta gatgaggaag gcgatgaggg cgccggccgt catttcgccg tcgatgacgc
     4201 tcatgccgcc gtaccagatg atgcccgtga cagcgatggc tgcgaagaac tggatgaacg
     4261 gcgtcagcag ggccgtcagt ttggtcgtcg tcatgagagc ccagaagttg cggtcgttct
     4321 ggaccatgaa gcggcggatt tcaaagtcct cccggttgaa ggagcggatg atgcggatcc
     4381 ccgaaatagc ctcttccaaa agcgacgtaa tgtcagccat cttgccctgg acgtcgtggc
     4441 cggcccggcg caggcgggag ccgaagaagt tgatcgtaaa gacgacgagg gggacgatga
     4501 ccaaggtcaa cagggtcagc ttccagtaga ggaagagcat ggaccccagg gagccgacga
     4561 ggatgaccgc ttcctggacg aaggaaatga ggttgcctgc aatggccgtc tgcaaagccg
     4621 tgacgtcatt ggtcaggttg ctcatgatat tgcccgtctt gtgcttgtcg aagtacgaca
     4681 gggacagggt ctgcaagtga cgatacaggg tttcgcggat atcgttgacg attttctggc
     4741 caacgaaact catgaggtat ttctggccga aatagaagaa cccgcggagc aggaagagga
     4801 ccaggatacc gatgacgatg aggttcagcg tgtagatatc cttattggcc aggaccttat
     4861 cgatgacgtc cttgatgagc cacgggacga cgacgtcgct cgccccggac agggccatac
     4921 agatgacggc gacgacgagg cgcatcttgt acggcttgat gaacattaat aatctcttat
     4981 aactttgcca actattgtat tgcttcatgg gcacctcttt ctcttgcaaa atctaagata
     5041 gcctgggccg tccgctggac agcgccgggt tcccccatcg tgcggcggac ggccgccagg
     5101 gatttgtgca atgcttcacg gtgcacttta tcggtgatca taggcaccat ggcggcagcg
     5161 atgttttccg gcgtcacctc atcctgccac aattcaggga tgacccgatg gcccatgatg
     5221 atgttcggca ggccgatgct gtcgatatgg accaggactt tcgataacca gtaagtcagg
     5281 cggttgacgc ggtacaccag gagcgtcggc aggcccatga gggccgtttc cagcgtcgcc
     5341 gtccccgaag ccgcgatggc tgccgtactg agattcatca tatcgtatac tttttcttca
     5401 ccgaccgtga cggcgacgcc tgaagcccgg atgatctcat cgaggaggcc ttcgtcgata
     5461 gtcgacgcct tgggcaggat gaactggacg tcccctgcct ctttgcggat gagcttggcc
     5521 gcttccagca tacacggcag gaggccgcgt acttcctgga cccggctgcc cggcatgagg
     5581 aggaccgtct tcttccgttc gtccagtccg aaatgagcac aggcctcgcg gcggctcatg
     5641 gtcgcatgga ccgtatcgac caggggatgg ccggcataga tgacattcgt ccccatgtct
     5701 tcatacatct tggcttcaaa gggaaagagg gaaatggcca gatccgtata cttggcaatg
     5761 gtcttgccgc ggctcttgtg ccaggcccag atggtcggca ggatataata gacgacgggg
     5821 atgcccgccg ctttggccac cttgatgagg cgcatgttga agcccggata gtcgatgcag
     5881 accaggacat cgggcttttc tttacgcatc gtatcgacga ggaagtcgcg gaggcggaag
     5941 aaaaagggaa tcttgcggat gatttccccg acgccgatga cgcctaagtt cttgatgtca
     6001 tagacgatgt cgacgccggc cgctgccatt tccttgccgc ccataccgag cagttcgatc
     6061 tgcggatcca gttcgcgcag ggaccgggcc aaattggccc cgtgcaggtc gcccgacgcc
     6121 tcaccggcag aaaacatgat tttcataggc tctcctaaaa attcttatcg acagcacaga
     6181 tacagatatc gtggcggttg gccaggtcca ggacttcgtc ctgttcgacg aagagcgtcc
     6241 ggccggcttc catggccagg acattgcaat ggctttcaat catggacaag atggtctgct
     6301 tgcccacggc aggcacgtcg aaacgcgggt cctggttggg cttggccact ttgacgacga
     6361 cggatttttc ccgtcccagg gcgccgccgc ggcggatgca agcgtcggtc ccttcgatgg
     6421 cttcgacagc catgacggcc ttgtgcttga cgacgacagt ctggccgatg tccatgccgc
     6481 ccatggcctt ggccgtgacg aaaccgaagc ggatatcgtc ttcttccgac gccgtcggtt
     6541 tacggcgcga caagacaccg caagacggca tgaggggttt caggtacagt gtctggtcga
     6601 cgacttccag gccatctttg gccagttctt cgacgatggc cagcatgatc gtatcgtcct
     6661 tgcggttgcg cagtttcgcc agcagcttga tagcccgcca gtcagggaac ttcaagcctt
     6721 tgaagaggat ttccttagtg accttgccaa tcatggtcac tttggcgacg ccattcttct
     6781 tgagggtctt gatgacctta tccagtttgg cgacgttgat ttcataataa gcatctgctt
     6841 cggcagccag ttccggttcg acatcactga cgacggcgat gacgacgact tcgtgaccca
     6901 gctgtttggc agcgcgcata aattctacag gcagtttgcc gacaccggca agcaatccta
     6961 ctttttccat agcaatcatc cttattcata aagagaaata ttcatcttta tattataaca
     7021 aaaaaacagg ccccctgtca gcaaaaaaag gggccatcgc agacgcgaca ccccttttta
     7081 gccaatagcg gctgcctaat ctttcgtccc tctgcagata ccgcggtcgg cattgcggag
     7141 gaagcggagg aaatgttcta cttcttcata gctgtccagt tcctgttcca tttcggcaat
     7201 ggcctggcgc aggttcaggc cggaacggta caggatgcgg aaagccttct tcagttcccg
     7261 gcgtacttct tccggaatcc cggcccgtga aaggccgaca ctgttcagcc cgatgcagcg
     7321 ggccggctga ccgtcggcga tgacgaaggg cggaatatcc tggacgactt ttgccaggcc
     7381 gccgatcatg ctgttgcggc cgatcttaac gaactgatgg acgccggcga tgccgccgat
     7441 gacgacgcgg tcttcgacgg tgacgtggcc ggccaggccg gcgcagttgc tcatgatgac
     7501 gtgattgccg acgacgcagt tatgagctac atgagtgcag gcctggaaga ggcagtcgtt
     7561 gccgatgcgc gtttcttcgc cttcaccggt ggccgtactg acggtgacga attcccggaa
     7621 caccgagcgg tcgccgatga tcaggtagct cttttcaccg tgatatttca aatcctgtgg
     7681 ttccgaaccg atggaacagc tcgggaaaaa ccggcagtcc ttaccgatcg tggtccagcc
     7741 gtcgatggta acgtgggcca tgatttccgt accatcgcca atggtaacgt ggggcccgat
     7801 aacggcatac ggcccgattt tgacaccggc accgattttt gcctgcgggt ctataatagc
     7861 cgtcggatgt atcatgctct gaggcatgtt attcgtttcc tcggtcatta aagtcactcc
     7921 tcgcttccct gcctgagcgg caggcgctta ttttcaacct aaatcacata ttatatacac
     7981 tcgcagaata ccagtccggt tttgccgggc aaaatcaggc cttttcccgc tatattcctc
     8041 tgtatactcg caaatacgcc catttaggcc gttatttaac atgcagtaaa gattttttaa
     8101 atttttctta acttattttt tcgtgttctg acccggcatg agggcaaaga ggtattcccc
     8161 ttcggcacag agcgtgtcgc cgacgtaaca ctgggccttg accttgccca tgcggccttt
     8221 gatcttttcg atgtcagccc gcatgaccag ctggtcgcca gggatgacgg gatggcggaa
     8281 gcgcagtttg tcgatgcctg taaagaacgg gatgaggccg cggttttctt ccggatagag
     8341 cagggctacg ccgccgacct gggccatggc ttcggcgatg aggacgccgg gcatgaccgg
     8401 ttttcccggg aaatgcccct ggaaaaattg ttcatcgaag gtcgcattct tgatgccgat
     8461 ggcatatttc atcggttcaa gttcgatgat gcggtctacg agcagcatgg gatagcgatg
     8521 cggcaaaatt tccataatat cagttacttc caaagtcatg agaattcctc cttaaatcac
     8581 tgcatttctt caaatatttt atgggtcagc aggttgttca gtttatggct cgatttgacg
     8641 gcgatgatgt gccctcggac aggacggccg agcagggaca ggtcgccgat gatatcgagg
     8701 accttatgac ggaccagttc gtcgtcacag cggggaacgg acaggcacga cgtcttatcg
     8761 tagacgacga cgttttcgat ggtaccccct ttgccgaggc ctaattcctt gagcatttcc
     8821 aattcttccg taaaggcgat ggtgcgggca aaggaaatgt cgcggacata cgattccttg
     8881 gtaatcgtcg tgtccaggaa ctgcacgccc agcatggggt gcgggttgat cgaagtgaag
     8941 gtgatgcgga agccatcata gggcacgatg gcgatgaatt tatcgtcctc ctggacgaga
     9001 tgctgttttt ggacggccag caggcggcgc ggtgcgtcca ggtcacggat tcccgcttct
     9061 tcgatgagct tgacaaaagt caggctgctt ccgtcggcga cgggcggttc gaccgaatcc
     9121 atttcgacga agcagttatc cacttccatg ccgctcaagg cggacagcag atgttctact
     9181 gtgaagacct tggcttcccc gttttccaac gtcgtcgccc gcattgtatt ggttacattc
     9241 gtgagatggg cttgtacaga cggtctgccg ggcaaatccg tccggataaa gacgatgcct
     9301 gtatcagccg gagccgggcg caataccata tgaacttcat tcgcagcatg caggccgatg
     9361 ccgttatatg aaacgtcatg agccagcgtc gtttgtacta ctattgacat aggcaggccc
     9421 ctcctttcct gttacgagta agataatttt tttcatactt ttttattata ccttatttat
     9481 agggtgtatg acaagggtgt tgttcgtggt ttcggcctgc ggcctagtgg tccgtggttc
     9541 gcggcccacc taattccttg caaatgaaaa agagccgccc caggggacag ctccttttcg
     9601 ttcaatcgct ttttacttca atagttcata gacagaggcg acgacgtcgt tccaggtttc
     9661 aatctgggcc tgttccccat ccatggcggc tttgccggct ttacactggc gggccacgga
     9721 ctgaggcgac gtgccgttat acgtgttacg ctgcttgacg caggtttcga tatccaagta
     9781 gtgatggatg tcttctgcga agagcgggct catggcctgc aattcctgca gcgataactg
     9841 ctgtaatacc ttgccctgtt cgatgcagta gtggacggcc ttgccgacga cggcatgggc
     9901 ttcccggaaa ggaaggcctt tcttggccag gtagtcggcc atatccgtcg cattggaaaa
     9961 gtcgctttcc agcacttccc gcatatgttt gccattgacg gtcatcttgt cgatcatggc
    10021 cgcatagatg gtcagggcga atttcaaatt atcgatggca tcgaagacac cttctttatc
    10081 ttcctgcatg tccttgtcgt aggccatggg gatgcccttc atcatggtca ggacgcccat
    10141 gaggtggccg tagaaccggc ccgtcttgcc gcggacgagt tcgcagatgt cgggattctt
    10201 cttctggggc atgatggacg agcccgtgca gtgggaatcg tcgagttcga tgaacttgaa
    10261 ttccgaactg gaccagagga tgaattcttc cgacaagcgg ctgaggtgca tcatcaggac
    10321 ggctgcgaat tccaggaagg cgatgatgta gtcccggtcg ctgacggcat cgagactgtt
    10381 gtcgtaaatc ttgccgaaat gcagggtctt ggcgacgaaa ggctggtcga ggggatacgt
    10441 cgtgccagcc agggcaccgg caccgagggg catgatatcg gccatgtccc aggcgtattt
    10501 caagtggatg aagtcacggg acagcatgga gaaataggcc atgaggtggt ggctgaagag
    10561 gatcggctgg gcccgctgga ggtgggtata gcccggcatg atgacgtcgc tgtacttttc
    10621 tgcagccttg accagcgatt cctggagctt gaccaggagc ttggcgacgg cggcgatttc
    10681 gcggcggaca tacatgtgcg tatccaaggc gacctgatcg ttgcggctgc ggccggtatg
    10741 caggcgcttg ccggcttcgc cgatggcatc ggtcagtcgt ttttcgatgt tcatgtggat
    10801 gtcttccagc tcgacggaga aatcgaagtt cccgtcttcg atctgctgga aaatagcctt
    10861 caagcccttg acgatggcgt ctttgtcttc ctgggagatg atgccctgtt tggccagcat
    10921 cgtcgcatgg gcaatgctgc cgcagatatc ttcagcatac atgcggcaat cgaactggat
    10981 ggacgcattg aattcttcta cacttttatc cgtggccttg gtaaaacggc cgccccataa
    11041 cttcatacga atcgctcctt atttaccctg tttttctacg tgctgtttca tgagggcccg
    11101 catcttgagc ggcaggccga agaggttgat gaagccttcg gcatcggtct ggtcatatac
    11161 gtcgtcttcg ccgaaggtga cgaattcttc gttgtacaag gagtacggcg atgtagaacc
    11221 agcgctgatg atgttgccct tatagagttt caatgtaacc tggccggtaa cggtcttctg
    11281 cgtttcatcg acgaaagccg acagggcttc gcggagcggc gagaaccatt tgccgtcata
    11341 gacgagttcg gcaaatttgt tggccacatt ttctttatag tgatacgtgt cgcggtcgag
    11401 gcagaggtat tccaattctc tgtgagcata catgatgatg gcaccgcccg ggttttcata
    11461 gatgccacgg ctcttcatgc cgaccagccg gttttcgacg atatcgacga tgccgacgcc
    11521 gttgcgggca ccgatttcat tgagggccgt aatgagttcg acagcaccca gtttctgacc
    11581 gttgacggca acagggacgc cctgttcaaa gtcgatcttg acgtattccg gagcatccgg
    11641 agcctgttcc ggcgtcttgg tgaccatgta gaccatgtct ttcggggcat tggccgggtt
    11701 ttccaggtcg tccccttcat ggctcaggtg ccagatgttc cagtccatgc tgtagcgata
    11761 gggattttct tcggactgat agtagatggg gacgttgtgg gcttcagcgt atttcagttc
    11821 gtcttcacgg gatttgaggt cccacatccg ccagggagcg atgagggtcg tttccggagc
    11881 cagggctttg acggtcaatt caaagcggac ctggtcgttg cccttgcctg tagcgccgtg
    11941 agcgatggca tcggcgcctt ctttcttggc gacttcgacg agtttcttgg aaataagggg
    12001 ccgagcgaag gacgtgccca agaggtattt gccttcatag acagcaccgg ctttgaccgt
    12061 cggccaaacg tactgttcca tgaattcttt tttgatgtcc atgacataga ctttggaagc
    12121 gcccgaagca taggctttct tttcaatggc ttcaaagtcg tccggctggc cgacgtcggc
    12181 gcaggcagcg atgacttcgg cgccaggata gttttccttc agccagggaa tgatgaccga
    12241 cgtatcgagg ccgccggaat aagccaggac aattttttta atatcttttt tcgtgttact
    12301 catgatgatt tcctcctcta tctgatgaac caaagcgatt gatttgtgtt tgtttctgtc
    12361 tacgtttgat agtatacacc cagttgaaat attatgcaag tattttgcat aaaaaatttg
    12421 caaacttttt ttacaggcct gtcaattccc tcgccctgca aagtatagta taataaaagt
    12481 gtttagtagg aggaattcaa tgaaactaaa atacatcgcc atctttgccc tggcctttgc
    12541 catctgtgcc ggctggaacc tgctcttcga cgcgccgaaa acggccaagc ctgccgtcga
    12601 gacggcggcc aagctccaag aagaaaagga accgcaggga agctggccgg caccgctgcg
    12661 gccgctggaa atccggatct atccgtacct gcacttccac gaagctatgg acaacaagat
    12721 caagaaaatc cccgtctacg tagccatcaa cgccatttcc aagcccatgc agcatgccgt
    12781 cgtcgccacg gaagaccgcc gcttctacga ccacggtgcc atcgacccga tcggcatcat
    12841 ccgggccgtc ctggtcaaca tcgaatccgg tgagaccgtc gaaggcggca gcaccatcag
    12901 ccagcaggtc gtcaagaatg tcttcctttc ccaggaccgg accatggccc gcaaggccca
    12961 ggaattcgtc atggccttct tgctggaaca ttactacagc aaagatgaaa tcctggaaat
    13021 ctatttgaat acagcctatt tcggcgccaa cgccacgggc atcgacgacg cggcccggac
    13081 gtatttcacg acgacgccgg aaaaactgga cctggcccag gcatccatgc tggccggcct
    13141 ggtacaggcg ccgacctact ataatcccct gaaaaattac gacgctgcca aagcccggca
    13201 gaaaacagtc ctgaccttga tggcagaaca aggctatatc agcccggaac aaggcgcaga
    13261 agcttataaa aaagagattc tcgaacgaaa gtagctgcta tcaggcggga cgccctcgta
    13321 acgagcatcc gccgtctgta gtaggggccg tcccaaaggg cggcccgctg tgagattcag
    13381 atacaggcca tttaggatga tggcaagctt ttttaccgcg agtcattcgt catggtaagg
    13441 gaatgcacat aattcaaggc gggacgccct atgggcgtcc cctacagcat aaaaaaggcg
    13501 ctgtcttcat gcgacagcgc cttttctgca gcttactggc ctttgaggcg gctcagtgta
    13561 tcctgggcaa aggccttggc ccggtccagg tgcaggtctt cccccgcttc cggccgatgc
    13621 atgtacttgt ccttgtccgt ccaggcgacg tacatcccta tgacgccgct gccaggcttg
    13681 aggagctcta cggcactgcg caggtgcttg gaggcctgat ccgaataggg atcgtcgtag
    13741 agcgtggcaa agacggcgac gtggtcgttg ccgattttct tcaacgcttc ctgcccttta
    13801 gggtccgcct ggttctcgtc gatccaaaaa ccggcaaaga cgcagtcata cgatgaaaag
    13861 tcatcgggca tgtcgtcgac actgacgcag ggcgtcccct gcggaaggcc tgcggcgata
    13921 gcatgtgcaa tttgttcaat acgtccggtc tgagaagaat acacaacaat ggcgttcatc
    13981 ataacatctc ctttttatat tttgatacta attatcgttt tatgtactac tagtataaca
    14041 taaaaaggct gccattgtcc aatagttaat ttataatttt aagctgtttc tttatagttt
    14101 aaagtaatac gtttaagtaa acctctcaga ccgccttcgg cggccagctc ccctatcagg
    14161 ggagcctttc gtcctgtcgc atgataacgt aaacgggcgc cccacgtggg cgcccctaca
    14221 acttaaactt atttagtttt cttcgatgac tttccccggg ttgaggatcc atttcgggtc
    14281 gacggcgcgt ttgatggtct tcatgaggtc caattcggcc ggatccgtat attcttccat
    14341 gtatttcagg cgcttcaagc cgatgccgtg ttcaccggac aggcggccgc ccaagccgta
    14401 gacgtattta tacgctttgg cccggaattc cttcagctgt ttttcccagt cttcgtcgct
    14461 catatcgcat ttgaggatct ggaaatggag gttgccgtca ccggcgtggg ccaggcagaa
    14521 gacgcggaaa tcgtagttct tgctttcctt gaccagggtc tggatgcagt cggcgatttt
    14581 cgtgaccgga acgaccaagt cttccgacgt ggcgactttc gagaagacgc gcgtgctttc
    14641 caggcagttg cggcggactt tccagacgcg gtcgtcggct tcgacgatgt ccgtagcccc
    14701 acattcttca cagacagcgg ccaatttttc cattttatcg tccaattcgt cttcattgaa
    14761 ggtttcgatg gaaatgatat cataacagcc atcttcgtag tgaggcagtt tcaagtcgct
    14821 gtaatcgctg gcgctgcgga cgaagttgtt atccatgaat tcgacagatg tcgggttgag
    14881 gccggcttta atcagtttcg gtaccagagc cgtcgccttg gccaggtccg tgaagacggc
    14941 caggacgtcg agtttatacg gcggcaaggg aacgagcttc aacgttgcct tggtgatgat
    15001 gcccaaggtt ccttcggaac cgatgataag ctggtccagg cagaagccgg ttgagcattt
    15061 ctttaaagta gcgccgaggt tgacgatctt gcccgtcggc aggacggctt caatgg
//
LOCUS       DAWCYS010000014        14959 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_263_length_15028_cov_9.946771, whole genome shotgun sequence.
ACCESSION   DAWCYS010000014 DAWCYS010000000
VERSION     DAWCYS010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14959
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_15028_cov_9.946771"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3098 a         3940 c         4367 g         3554 t
ORIGIN      
        1 acgcaggcgc gaaggactgg ctatcggggg cctggggcgc ccacgggcgc cccccgcttc
       61 ggggggcggg ggacgatttc ccggccagcc tgctgagagt atcatccaaa ttcctaacgg
      121 gtcgcccacg tggcgacccc tacaatgcca aaaagctccc cttcacaggg gaggcaggtg
      181 gctaacggct aacagctaaa aaggtctgtc gcatcgcgac aagacctttt ttattacttg
      241 ctcaaatcaa gagagcgtac tttttcacgg agcggcagga ccgacggtgg gctgacggag
      301 agttcgtcta cgcccatgcg caggaacgtt tcggtcagtg tcaggtctgc accgagttcg
      361 ccgcagatgc cgacccagat gccggctttg tggccatttt cgatggtcat ctggatgagt
      421 ttgaggacag ccggatgatg gggatcgaag aaggcatcga gctgggtctg ctgacggtcg
      481 atggccaggg tatactgggt caagtcgttg gaaccgatgc tgaagaagtc gacttcctta
      541 gccagttctt cactcatgac ggcagcagcc ggtgtttcga ccatgatgcc gatttccata
      601 tctttatcgt aggcaatgcc tttttcatcc aattcctgac ggacttcgtt aaggatttct
      661 ttgcaccgct gtacttccca gacggaaatg atcatcggga acatgatggc gatcttacca
      721 taagccgaag cgcgcaggag agcacggagc tgcgtcttga agaggtcgac gcgggtcagg
      781 cagatgcgga tagcacgcat gcccatggcc gggttttctt ccgggtcgag ctggaagtag
      841 tcgactttct tgtcggcgcc gatatccagg gtacggatga cgacggtctt gccctgcagg
      901 gcttcagccg cctgtttata ggcttcgaac tgctggtctt ccgtcggata atcgctgctt
      961 tcgagataca ggaattcgct gcggaagagg ccgatgcctt cggcatcgtt ggccagaacc
     1021 tgcggcagat tgccagggtt gccgatattg gcatagaggt tgattttctt gccatctttg
     1081 gtacccgtcg ggacgccgcg tacctgttcc agagcggcta cgcgaagttc ttcttccttc
     1141 tgtttctgct tcatggccgc catggtttct tcatccggat tgatgtagac cgtgcccgtc
     1201 gtaccatcga tgatggccgt catgttgtcg acgtcgttgt ggagctgtgt gcccgtgctg
     1261 acgacggcag cgatgcccag ggtgcgggcc aggatggccg tatgcgagtt cgtgctgcct
     1321 gctgacgtaa caaagccgag aatcttatcc gtatcgagct gtaacgtttc gctgggagcc
     1381 aggtcatcgg ctgcgatgat gacgtggtca taattgctga agtcgattcc cgggccgccg
     1441 caaagctgct gttctacacg gcgggagatg tcgaggacgt cggctgcgcg ggcctgcata
     1501 taagcatctt ccatggaacg gaacatttca gcaaatttct gagccgtctg gtcgacagcg
     1561 gcttcagcat tcattttttc atcgcagatc ttggtttcaa taccttcgat atagtcatcg
     1621 tcatcaagca tcatctgatg gacttcaaag acagcggctt gttcttcacc gactttgtct
     1681 acagccttga catacaagtc cttgagctgg ccgatagctt cttcgcgggc tttctggaaa
     1741 cgcgcgattt cggcatcgac gtcttcgatt ggccgtacgg ccgtagaaat cgtattacga
     1801 tggaacagtg ccagcggtcc gatgctgata ccggagaaga cacttttacc ttttaacgtc
     1861 agcatgtact ttccccctca aagaaattag aagttttctt tcaagaaggc ttctacagta
     1921 ctagctgcct gttcttcgtc tgcgccttct acttgtactt taatcgtttc gccctgtttt
     1981 acgccgagag ccatgacggt gaagattttc ttcaaattgc ctttgcgggc gcctttgaat
     2041 acgctgatat tggattcaaa tttctttgct tctttgacca aaaggcctgc cggacgagcg
     2101 tggataccct ggggatcagt gacaacaaat tcaaattctt tcatgacaat tcctcctttt
     2161 aggaaatata gaagatgaga taaagctaca taatatagtg tatacgattt atagcgataa
     2221 cgcaatcagt cttgcggacg ttttttgcgg aaaataccga gcaggatagc tgcgacgatg
     2281 acaccggcca ctaaggcgac gatgtacata ggccagttgg tgatgacacc gaagacgaag
     2341 ataccaccgt gaggagccgg aagggcgcag ttgaagagca tcgacaaggc accggcgaca
     2401 gcggcgccga tagcgcaggg cgggataacg tggaccggat ccgatgcggc gaaaggaatg
     2461 gccccttcgg tgatgaagga caggcccatg atgaagttgg taatcgtcga ctgtcgttct
     2521 ttcggtgtga atttaccttt aaagatgagc atagccaggg cgatagccag aggcggaacc
     2581 atgccgccga ccatgaccga agccatgatg cccgagctga cagcctgacc gctggcactc
     2641 atgagcgagg ccgtaccgaa gacgtaagcg gctttgttga tcgggccgcc gaagtcgatg
     2701 gacatcatgc cgccgaggat gaagccgagg acgatacggc tcgatgtggt catgctgttg
     2761 aggacgtcga agagccacgt attgaaaaca gacgtcggcg gattgatgat ataagtcatg
     2821 atgacgccga tgaggaccag gccgaggacc ggatagaaga ggatggactt cgtgccgtcc
     2881 agcgcttccg gcagatgggc cagggctttt ttcaggaaaa tgatgagata gcctgcgatg
     2941 aagccggcaa agagggcgcc gaagaaacca gaaccgcctg tcgttgccag gaaaccgccg
     3001 acgataccgg gcatgagagc cggcctttca ccgatactca tggcgatata gcctgccagg
     3061 ataggcatca tcatgctgaa ggacaggccg ccgacatttt tcaggaaggc agccagcggc
     3121 gtgccgctgc cgaagttggc cgtaccggcg ttatccatgt cgcagaggaa agccagggcg
     3181 atgaggatcc caccgccaat gacgaaggga agcatatggg atacgccgtt catcaagtgc
     3241 ttataaatct gatggccgac gctttctttt tcagcgcttt cgacatcgac ggcttccgac
     3301 gacgattcat tgccctgata aatgggtgct ttgccgtcga ggacggtctg gatgagttct
     3361 tccgggatct tgatgccgtt ggcgactttc gtgaagatga ccggtttgcc ggcaaaacgc
     3421 tggaccggca cgtctttatc gcaggcgacg atgacgccga cagcgcggtc gatatcggcc
     3481 tgggtcaggt ggttcttgac accgccggaa ccattcgttt cgattttgat gtcgacgccc
     3541 atggcagcgg ctttgttttc cagggcttct gctgccatgt aggtgtgggc aatgcccgtc
     3601 gggcaggctg tgacgccgat gacgtaggga cgatccgacg gagcggctgt ttccgtttca
     3661 gccggtgctt cttcggcagc ggcttcagcg gcagcttctg cttcttcagc tgcgacttgt
     3721 tcggcttcgg ccttgtcgat gatagccagg tattcttccg gcgtctgggc cttcatcagg
     3781 ttgtcgcgga aatcgtcgtc catgagcata cggctcagac ggctcaagac ttccagatgg
     3841 acgttggcac cgccagccgg agcggcgatg aggaagaaca agtgagccgg ttcgtcgtcg
     3901 aggctttcgt agtcgacgcc gtcgcggaca atcatcgaag ccaggcccgg ttcgttgacg
     3961 gcttccgtct tggcatgggg aatggcgatg ccttcgccga tacccgtact gccttcttgt
     4021 tcacgggcga ggacgcgctg cttgtatagc tctttgtcgt tcaggttgcc gccggcggcc
     4081 atgaggtcga ccaggtggtc gatggcagcg gccttgtcgg ctacagacgc attgaggtcg
     4141 atgctctgtt tcttgagtaa atctacaata cgcattattt ctctccttct cttctcaact
     4201 ctctacacaa aaatgcgtgt ttctttataa ggtagcgtat acttccttga tatcgtcttc
     4261 cgtagccagc cattcatgga aggccgatgc actgccggcg ctgatgccca ttttcaaggc
     4321 ttcctggagg tcgcccttgg acgcttcgaa accggccagg aacccagcga ccatgctgtc
     4381 gccggcaccg acggagttga ccagcgtgcc tttcggggac tggcggcggt agacctggcc
     4441 gtcttcgccg aggagcaggg cgccgtcacc gccgagagat acgaggacgt tgcgggcccc
     4501 catggcctgg agctgtttgg cgcagacgat gatttcttca tccgtatgga ggtcgacgct
     4561 gaacaattcg cctaattcaa agttattcgg tttgatgagg aacggtctat atttgatgac
     4621 attcttcaaa aggtcgcctg tggcatcgac gacgacgcgg atatcgcggt cctggaggcg
     4681 tttgaggatc tgttcgtaaa tatcatcggg aatatcgctg gggatgctgc cggccagtac
     4741 cagggtgtca ccggctttga gggtatcgat ctgagccagc agggcttgcc gtttttcttc
     4801 gggaatatgc ggtcccatgc cattgacagc cgtttcatca tcggcgctga ctttgaggtt
     4861 gatacgcgag cagccttcgt cgaggatgat gaaatccgta tcgccgccgt tgattttggc
     4921 caggcgttca atttcccggc ctgtgaaacc agcgacgaaa ccgagcatct tagaaggaat
     4981 atgaaggttt cccaggacga gggacacgtt gatgcctttg ccgccaggat agattatttc
     5041 ctgttcggcc cggttgatgt caccggccgt gaaggtcttc atgtggacga catagtctaa
     5101 cgatggattg aacgtaactg tataaatcac aggatatgca cctccgtaat ctgctgataa
     5161 ggccggtaat cgacgtgttc ttcatctttg gccgtaatca gcgtaaccat tgataagtct
     5221 gcaaatgtaa tgtgcgaaga taaaccgaat ttactggaat cggccaggac gaagcggtct
     5281 aggcactggc ctacggcagc gcgcttacag acagcttctt catcatccgg cgtcgtacat
     5341 ccggaatggg gagcgattcc attggctccg aagaagccct tggtgaaatg atagcggccg
     5401 atcatatccc gcatatcact gccgacaatg gcttccgtat cgcctttgac ccgtccgggc
     5461 aggatgtaga cgcggctgcc gctgcgcccc aatttctggg ccagcgggat gctgttggtc
     5521 acgaaggtcg cttcagattc ggccaggaaa tcggccatct gttccgtcgt cgaaccggcg
     5581 tcgagataga cgaagtcatc cttcttgatc aggctggctg catactgggc gatgcggcgt
     5641 ttatcgctca tatgaatgga atacttgtcc tgcagatttt ccatatccgc ttcatataaa
     5701 gaatcatcgc gcagcgtcgt ggcgccgcca tggacacggc gcaggcggcc ttcccggtcc
     5761 agcgtgagta aatcccgacg gaccgtcgat tcggaaatgt tcagaaaact cactaagtcc
     5821 tgaaccgaaa cggcaccact cgtattgaca agacgcatga tcttttcatg tctttcttct
     5881 gtcaacatcg tatcacttcc ctttttatcc tcctttggcc tcattatacc gttatattca
     5941 atcattttca atcaaattca ttcattataa tcaaaaagca atcattttcg ttcatctatg
     6001 cgtccggctc attaagtgat tttattcaca tatgtagggg ctcgcacgtg gcgagcccgc
     6061 aggccgccgc gacatcacag atgtcgctgt aggggccgtc ccaaagggcg gcccgttgtg
     6121 ggaatcccgt gaaatgctgt tcgtcgatat cattattcgc agttcacagg tcacaaaacc
     6181 atttctcata gaccagttga gtatatctcc cattgttcac aacgggacgc ccaaggctcc
     6241 ctttataggg gagctggccg ccgaaggcgg tctgagaggt taaagagcgt cccctacgca
     6301 caaggccata ggccgtgaat gatttgccgt taatccataa cggttctgcc ataacatcgc
     6361 ctatggtaat gcgttcatcg tttgtcgtaa atccataaca tcgcctggct gcgattcgtt
     6421 catcgttcgt cgtcatcgaa aaaaaagctg ccgcatagcg acagcttttt tcgttgtagg
     6481 ggcgacccgt ggggcgcccg cgtgcggccc cctacgacga acgacctacg ataaacgatc
     6541 atcaggccta cgatgaacga tcatccccgt gcttctgcga cgcgggcgat gccgtggcgg
     6601 gtgcggtatt tgaggaagag cattagggcc agggcgacgg tgaaggcgcc gacgaagaag
     6661 tacaaggtga cggcataact attggtattt tctttgatga gcgacaggac cagggggccg
     6721 gcaacgccgg cagcgcccca ggccgtgagg atgcggccgt ggatggcact gaggtatttc
     6781 gtgccgaaga ggtcgctgag gtaggccggc atgcacgaga agccgccgcc atagcaggaa
     6841 atgatgagca ggaccaggac ctggaaactg aaggcgcccg taacgccagc cagctgccag
     6901 aaggccagga tttcaatgac aaagaagagg acatacgtag ccgagcggcc gatgtagtcg
     6961 gatatggtcg cccaggcaat gcggccggcg ccgttcatga ggccgatgaa gccgaccatg
     7021 gaagccgctg ccataggcga catgccgacg acatcctgtg ccatgggcga agcgacggac
     7081 agcagggcga taccgcaggt gatgttggtg aagaagagcc accagatggc gtagaactgc
     7141 caggtcttca tggcccggcg gacggtgaac tgcggctggg cgtcggcgta ttcgacggaa
     7201 gattcttttt cggccttggc cggaacgtct ccggctgccg gcggagccag gtagagcgac
     7261 gaagccgtca tgacgaccat atagacgcag ccgaggatga tgaagttagc gactaagcca
     7321 aaatgctgga ccagcagctg aatggccgga ccggcgatga ggctggcaaa accgaagccc
     7381 atgatagcca ggcccgtagc aaagccccga ttttccggga accatttgac cagtgtcgat
     7441 acgggcgtga tatagcccgt ccccaggccg acgccgccga tgacgccgta aaaagcgtag
     7501 agcatgggca ggctttgggc atagagagcc gctgccgtgc cgaacatggc cgtggcgaag
     7561 caggtcatgg ataagaggcc ggacttgcgg ggaccgattt tctggacgaa actgcccaaa
     7621 aagccggccg acaggccgag gaagaggatg gccagggaaa aggtccacgt cgtttccttg
     7681 agggtgaagc ccatagattc catgatgggc ttggtgagga cgctccaggc gtagacactg
     7741 ccgatacaga ggtggagccc catggcagac agggcgatga gccagcgatt tcgtttcatt
     7801 tagacaacac tccttgtgag gagccagact ggcgaaaaaa aataccggcc gcctggctca
     7861 agaatatgta gcatactctt gcccagacgg tcggcatgaa aatctccgta cagttcatgt
     7921 ggttactcca cttgattccg tcagtcctgt acgaatctgc tgatgtgctt attatacgaa
     7981 tatggacggt gattgtcaat atcccttttt taacttttaa gcagccggcg agaagggaac
     8041 gtaaggctgg ctggaaacag ccggcttact ggtcttcgcc gtatcagctt tggcagcggt
     8101 tttcgcagtc gtcttggctg ccgttttagc agctgtttcc gttgcggtct ttaccgttgt
     8161 agctgttttg acaggcgctt tggccgcagc cgtggaagcg gcagccgtag cggcttcctg
     8221 cttctgctgc gatgccagtt cagcagcctg ggcctttttt tccctgatat ccgacaatgt
     8281 ctggcctgcc ggtgcctgac gcaggatata gacgttttct gccggttcga ccggtttcag
     8341 gacggaaaaa ccataggcca tcaatttctg ggcatcgccg aagcggtcgt ccgagttcat
     8401 gatggaagcg atgaccgtcc ggccatcctg ggtagccgaa gcgaccaggc agggaccgcc
     8461 ggcgttggtc gtgccggtct tgatgccgtt ggcaccgggg aagccgctgg tcaggaaata
     8521 gttcgtcgtc gtgcagtgct tggacccgcc gttgatatag ggcatgtcgt attcactgtg
     8581 gctgaccatg tcgcggaatt ccggcatttt catgccataa gcggcaatcg tcgccatatc
     8641 gcgggccgtc gaatagtgat cgctgtccgg caggccatgg ggattggcga aatgggtatt
     8701 cgtgcagccc agggcggctg ctttttcatt catcttggcg acgaaatcgg cctgggtcgg
     8761 cgtaaccgtt tcggctacgg caacggccgc atcacagccg gatacggtca tcatgccggt
     8821 cagggcatgg cgcagggtaa tctggtcgcc aggatcgatg cccagaacag acgcatcgct
     8881 ttcgatgttg tagacgtcat tggtgatgtt gaggggctgg tccattttgc cctgggcctt
     8941 gcccaattcg acgccgagaa taccggtcat gattttcgtc gtactgcccg gataggtccg
     9001 gtcgtcggca tttttcgcat agagcgtgtc acccgtatcg gcatccatga cataggcggc
     9061 atgagcgctg atttcaggtg gctgcggcgt atcggcaaag acggcggccg acacagtcag
     9121 gcaggcggct gcagccagac agaagattct tttcaaagac atagcataca cttctttctc
     9181 tataagggat ttttcgcagc catcttgggc ttgcggggat actttttggg gctttccttc
     9241 actttctcga tgtaaaggac ggcgcggacg tcatcgagtc ccggcaggtg aacaggtcgg
     9301 acttcggcga ttttgccgcc caggatctgc aaggcttttt cgccttctgc cagttcgtct
     9361 tcatactggg cccctttgag ggaaatgaaa tagcctccgg tcttcacgta gggcagggcc
     9421 cattcggcca atatgttcag gcgggcgacg gcccggctgg tgacgatatc gaaagcgctg
     9481 cggtattccg gcttatgggc catttcttcg gcccggccgt ggaggaacga cgccgtcagt
     9541 cctatatcgc tgaccgtgtt ttccaggaag gtcaggcgct tttgcaggga atcgaagaac
     9601 gtcacgttca agtccggccg gtagatgagc aggggcaggc cggggaatcc ggcgccagtc
     9661 cccaaatcga ggacacgagc gccttctttg aaataagccg ggtcataaca ggacaggctg
     9721 tcggccatgt gcttgatggc gacgcctttc gggtcggtaa tggccgtcag gttcatgact
     9781 ttattcgttt ccaccagtct ttcattaaaa gcgcagaact gggaaagctg cccgtctgtc
     9841 acggtaaggc ccatggcctt cagggcctgt tctaattcac tgcgaaacat gatttttttc
     9901 ctcccggctg tgctgttcca gtacgatgag caaggccgtg atatccgccg gggacacacc
     9961 ggaaatacgg ctggcctggc ctaaggacaa gggcccgatt tcatcgagtt tctgccgggc
    10021 ttcggccgac agggtgtcga tatcggcata gacgatatcg gcgggcatct tcttctgttc
    10081 caggcggttc atcttttcga cttgttccat ctggcggctg atatagcctt cgtacttgat
    10141 catgatttcg atttcttctt ccacgtcggc agccagttcc ggcaggtcga agaccgtccg
    10201 cagggcctcg tagtcgacat cgttgcggcg caggaggtcg taggccgtga tggccgtgtg
    10261 gatgggtgcc gtgcccaggc gttccaattt ttccagattg gccttggacg gcgtgacgga
    10321 aatggcctgg agggcggcga tgccttcttc gatggctttt ttcttggccg tgaagcggtc
    10381 ataccgttcc tgcgtgacca ggcccaggtc atagcctttc tgggtcaggc gcaggtcggc
    10441 attgtcctga cgcaggatga ggcggtattc gctgcggctg gtcatgatgc ggtacggttc
    10501 attcgtcccc tttgtgacca aatcgtcgat gaggacgccg atataggctt cggagcgggt
    10561 taggatgaac ggttctctgc cctggacctt caaggccgcg ttgatgccgg ccatgagacc
    10621 ctgggcagcg gcttcttcgt aaccgctggt cccgttggcc tggcctgccg agaagaggcc
    10681 agaaatcttc ttcgtttcca gccacggcgt gagctgcgtc gggtccaggc agtcatattc
    10741 gatggcatag cccgggcgca tgattttgac gtcttccagg cccgggatgg tacgcaggaa
    10801 ggcgtactgg acgtcggcag gcatactggt gctcatgccc tggacgtaca tttcttccgt
    10861 cgattcccct tccggttcga tgaagagctg atgccgcttc ttatcggcaa agcggacgat
    10921 tttcgattcg atggacgggc agtagcgcgg cccgatgccg gtgatgatgc cattgcacat
    10981 gggagcccgg tcgaggttgt cgcggatgat cttatgcgtt tcttcgttgg taaaggtcag
    11041 ccagcaacat tctttgttgc ggtctttccg ttccgacagg aaggaaaagg catgaccgtc
    11101 tttgtcgcct tcctgcttgg ccatttcatc ggtccgcagg gtacggcggt cgacgcgggc
    11161 cggcgtaccg gtcttgaagc gcataagctt gaggccggcc ttgaccaggg accccgacaa
    11221 atcttcagcc gagcgctggc cgatagggcc gccggaatag gtacaggtgc cgatgatgat
    11281 cttccctttg aggtaggtcc ccgtcgccag gatgacgcac ttggctttat aaacttcatc
    11341 cagttccgtc cggacaccgg tgacggcgcc gtcttcgacg agaaggtctg tgatcatgag
    11401 ctgcttgaca tcgaggtggt cctggttttc cagggtcttg gtcatggcca tctggtacat
    11461 cttcttatcg gactgggccc ggagagaata gacggccggt cctttgccgg tgttgagcat
    11521 gcgcatctgg aggcacgtct tatccgtatt gatgcccatt tcaccgccca gggcgtcgat
    11581 ttcccggacg agatggctct tccccggtcc gccgacagcg ggattgcagg gcatgagggc
    11641 aatattatct aagttcagcg tcgccatcag ggtcttggcg cccaggcggg cactggccag
    11701 ggccgcttca cagccggcat ggccggcacc gatgacgatg acatcgtatg tatcgactac
    11761 aaacatggtt acaccccttt atttacctaa acagaatcgg ctgaatattt cttcgaccat
    11821 atcgtcgtgg accgtatcgc ccgtgatcga gcccaaaagc tcccaggctt cgcgcaggtc
    11881 gacgacggca cagtcgacgg gcataccggc ttcgatggaa tcgagggccc gctgcagggc
    11941 ttgccggctc tggcgcacca gttcgatatg gcgcgtattg gtgagcaggg cactgcctgc
    12001 cgtatcggca tgggacaggg ccagggacaa caatttatct tttagggaat ccaggccgtc
    12061 acccgtacca gccgacaagg agagaacggg atgaccatag cctgccaaat ctttttccgt
    12121 aacgactggc attaaatcgg tcttgttgag gacgaccagg acattcttat ccttcgtgtc
    12181 ttccagaatg gccttatctt catcggacaa ggaccgggac cggtcgatga cgaccaggat
    12241 aatatcggct ttgtccatat aggccttggc cttggtgacg cctatctttt cgacggtatc
    12301 gtctgtttcc cggatacctg ccgtatcgac gaggcagacc gggacacctt tgagggaaat
    12361 ccattcttcc aggacatccc gcgtcgtccc cggcacatcg gtgacgatgg cccggtcttc
    12421 ctggagcagg cggttgagca ggctcgattt gccagcgttc ggcgtaccgg caatggcggc
    12481 catgacgccg tcgcggatga tgcggccgtc gtgagaagcc tccagcatat cgtccagggc
    12541 cgtcagctgt ttgcggatag ctccggctac atcggggacg gtgatatctt ccaggtcttc
    12601 ttcaggataa tcgatggtga cttccaggcg ggtaatgaag tccgtcaaat catggcgcat
    12661 ggcaccgatg actttagaca gccgcccttc aagctgattg acggcactgg tcaggccctg
    12721 gcggctcttg gcctggatga catccatgac ggcttcggcc tgggccagat cgatgcggcc
    12781 attgacgaag gcacgctgcg taaattctcc gggattggcc atgcgggcgc cgtgggccag
    12841 gaccaggccg aggatttctt ccaaggcctt gcggccgccg tggcactgga tttccagtac
    12901 gtcttccgcc gtataggagt gcggcctttt catgatgagc atgagcactt catcgacagg
    12961 acggccgtca tcggcaacga tgaagccatg ctggatagaa cggtcctggc gctgggccag
    13021 gggctttccc gaagggctgc ggaaaatggc atcgcctacg tcataggcat ggctgccgct
    13081 gatacggatg acgccgatgc cgctttcccc caggggggtg gcaatggccg cgatcgtatc
    13141 ggtcgtatcg tacatatggc aatactcctt tcatagccaa ggggctcgca tgaagtttcc
    13201 tgacatcatg caagattcct tttactttat agtttgtagt acagagtttg tagaaaaata
    13261 ctcgaaagag gctgtctgaa tgcgacagcc tctttgttaa gcttcatagg gcaaactaaa
    13321 aattattctt gatagtaaat gacgacgtgg cggtacgggt cctggccttc actttccgtg
    13381 cggacctgcg ggtcgttctg caaggtcaca tggatgattt tgcgttcata accgttcatc
    13441 ggttccagga cgacgcggtc gccgttcttc ttgacgcggt tcgccagacg gatagccagc
    13501 tgtttcagcg tttcttcccg gcgatggcgg taattttcga catcgagcat gatgaaatgg
    13561 cgcgtccttt cgccctgatt cgtcgtcaag ttggtcaggt actggagggc atccaaggtc
    13621 tggccatgct tgccgatgag gatacccaag ttcttgccgt gcaggtggag caggacttta
    13681 tcggacttga tcatcttttc gatgacgacg tcgatgccca tgttcttgag cacttcctga
    13741 aggaaggcct tcgccttggc ggcggcttct tcagccgtaa aatcatcgcc tgtttcggct
    13801 tcagccggtt ctgtttctac ctggggaacc gcttcttcag ctgcgccagc tgcgttggca
    13861 gccggttcag ccgctgccgg tgcgacgggc ttttccacgg ctgccggctg gctttccatg
    13921 gtcgggaccg tcggtacgcc ttctttttcg gtaatgcgta ctttcgccgg cttgctgccg
    13981 aaaccgaaga aaccgctctt cggttcttcc aagacttcga tgtcgacttc gtccttcatt
    14041 ctgccgagac ggaccagacc ggagcggacg gcatcttcga tcgtttttcc tgtagtttcg
    14101 atggtactca tgcttctgat cgtgctcctt ttttcttgag gtgggtcatg aggaactgct
    14161 gtgcccactg gaatacgtta gaaacaaccc agtaaatgac caggccgctg gggaaattca
    14221 agctgatcca gccgatgaat aacggcatga cgatcttcat gattttctgc tgtttagcct
    14281 gtgcatccga agccgctacc tgcgtacctg acatctggtt ctggatgaag taagtcgaaa
    14341 cagccgacag caccggcatg atatatgtgg ggtccggctg gcccaggctg ggaagccata
    14401 agaaactttc aaaggccggg tcgtagggat attcccgaag agcatagaaa atggcgatga
    14461 ggaatggcat ctgaatgagg atcggcagac agcccgacaa ggggttgacg cccatttccc
    14521 gatacagttt ggtcatttct tcctgcatct ttttctgatt gcccttatat ttcttctgta
    14581 attctttgat cttaggctgc agggtctgca tgccttccat ggaacggatc tgtttagccg
    14641 taagcggagc cagaaccagt ttaataatca aggtgagcat gataatggca gcgccatagc
    14701 tcggatagcc tacattctgg gtaaatgcat aacagtaatt catgaatgtc gtcataattc
    14761 cgctgaggac atccattatt ccactgaaaa agcccaatac tctcaactcc tgttcagttg
    14821 taaaaatagg gatctacggt accggatcat atccgccttt gtgaaaggga tggcatttta
    14881 aaatgcgcct tagtgccaaa taactccctt tgacagcacc gtacttctgc aaagcctcca
    14941 tggcataggc ctttctcta
//
LOCUS       DAWCYS010000015        14879 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_267_length_14879_cov_7.106854, whole genome shotgun sequence.
ACCESSION   DAWCYS010000015 DAWCYS010000000
VERSION     DAWCYS010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14879
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_267_length_14879_cov_7.106854"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3355 a         4125 c         4083 g         3316 t
ORIGIN      
        1 tgtaattact gggttttcag ccttttactc ttttttaagt gtcaaacttg ggattgtaac
       61 ataaacgata accctgtcaa ggggagatta gggttagtgg ctcgtggaga gcaccaccaa
      121 atcccaggcg ggtcgcccac gcggcgaccc ctactacaaa ctgcaaactt aatactcaaa
      181 aaggcgctgt cttcatgcga cagcgccttt tttcactctc tatttttctt cgtcagcctt
      241 tttttcttct tcgtccttat cggtcaggcc tgtggtcagg ccgcctttgg ggacagggtt
      301 tccccagggg ctgatctgta cttctgtctg ttcggctttg ggagctgccg gtgcaggtgc
      361 ttcttccttg actgtcggag cggcttcgga aacggctgct gtgtccggtg tttcggcttc
      421 tgccgtttct gccggtgttt cagcagcttc ttttttagcg tcctgcggcc cttccaaggt
      481 gccgtatttg accaggttat cgacttgctg atgattgatc gtttcgactt ccatcagggc
      541 attggccata tttttcagcg tatccaggtg ttcccgcaac atgtggagca cgtcggcata
      601 ggcttccgat acggtgtcgt gcatttcctt gtcgatgagt tcggctacgc tttcgctgta
      661 gttgcgttcg tgacccaggc tcttgccgag gaagacctgg tcctgctgtt cgccaaagac
      721 catggggccc agtttcgggc tcatgcccag gcgggtgatc atctggcggg cgatacgggt
      781 gacgctctga aggtcaccgg aagcgccgct gctgatttcg ttgaaaacga tttcttcagc
      841 agcccggcca ccgagggcga cgcggatctg ggccaggtag tacgacttcg tttcatacga
      901 ccggtcttct tccggcagca tcatggtata gccgccagcc cggccgcggg ggatgatggt
      961 gaccttgtgg accgggtcgg catcaggcag gagataggcg atgagggcgt ggcccgattc
     1021 gtggactgcc gtcaggcgct tttccttatc gctgatgaca tggctgcggc gttccggacc
     1081 gaagcagact ttttcgctgg cttcttccag ttcagccatg ttgatgactt tcttattagc
     1141 ccgggcagcc aggagggctg cttcattgag caggttgccc aagtcggcac cggtgaagcc
     1201 cggcgtcttc ttggcgatga cgtcgaggtc gacatccttg ccgaggggct tgcctttggc
     1261 gtggacgtcg aggatagccc ggcggccgcg caggtccggc cggtcgacgg tgatctgccg
     1321 gtcgaaacgg cccggacgga gaagcgccgg gtcgaggata tccggacggt tggtcgcagc
     1381 gatggtgatg atgccttcgt tggaaccgaa gccatccatt tcgaccagga gctggttcaa
     1441 tgtctgttcc cgttcgtcat gaccgccgcc aaggccagca ccgcgctggc ggccgacggc
     1501 atcgatttcg tcgatgaaga cgatgcaggg cgcgttcttc ttggcctgtg tgaacaaatc
     1561 acggacacgg gaggcaccga cgccgacgaa catttcgacg aagtcggaac cgctgatgct
     1621 gaagaagggg acaccggctt cgccggctac ggcacgagcc aggagggtct tgccggtccc
     1681 cggagggccg aagaggagca cccctttggg tatcttggcg ccaatggcat tatacttgct
     1741 ggggttgcgc aggaattcga cgatttccgc caattcctcc ttcgcttcgt cttcaccagc
     1801 aacgtcttta aaggtaacct tgatcttacc ttccccgtgc atcttggcat ggctcttgcc
     1861 aaagttcatg acccggccgc cgccgccttg ggtctgctgc atgatgaaga accagatggc
     1921 aatgaggacc aggattggca ggactgctgt cagcatgttc atccaccacg aaggctgttc
     1981 gggcggtttg gcgatgatgt tgacatcagc atcgcgcagt gccggcaaga gtgccgaatc
     2041 ggtcggggca taagaagtaa aagaggttcc gtctttcagc tgtccgacga tagcgtggtc
     2101 ggctgtgatc tggacggcat cgactttttt ctgctgcacc tgactcatga aattcgtgta
     2161 agaaatttcc gatttatctg tcttactgct ggtaaaggca tctatgatcg atacggcgac
     2221 gatgatgatg agtaagtata aacttacatt ccgcacaaat ttactcaaaa tatggctcct
     2281 ttcacttagt ttatgataat gcttttttat tccaaccaat acagtcttat attaccacac
     2341 ttcttctaaa gtaacaacta cttggaatat acttcttctt tcaaaatgcc aatgtacggc
     2401 aggttgcggt agcggctgtc gtagtcgagg ccatagccga cgacgaaggc atcggggatt
     2461 tcaaagccgt tgtaatcgac agggacttcg acctggcggc gggccggctt gctgaggagg
     2521 gttgccaatt tgaccgagcg ggcaccccgt tcctggagca tggggacgag atggctcaag
     2581 gtgacgcccg tgtcgatgat gtcttcgacg atgaggacat ctttatcctt gacgtcgtta
     2641 tctaggtcct tgcggatctt gaggacacct gacgacgtcg tcgacgaacc gtagctggag
     2701 cagctcatga aatcgaagta gaccgggccg tcgatgcagc gggccaggtc ggtgaaaaag
     2761 gtgacggccc ctttgaggat gccgcacatg aggatggtct tgccggcata atctttagta
     2821 atctgcgcac ccagttcgcg gacgcgggca gcaatctgtt cttctgtaaa taaaatttct
     2881 ttgatatcct gatgcatctt gttttcctcc gtgaaaaagt gaattacccc tgccgtcccc
     2941 tgcggggaac ggcaggaccc aggcagaggg ctgtccgggt cgtataagca caggcccggc
     3001 cgcactggaa ctgttttcca gctcctttgg cggccaggtc ccgtatcgtt tcaacataat
     3061 gaaaaggcag gtcctgacga gagcccgtcg cctcctgcca caagcggcgg atgaggcgcc
     3121 gctgcatagc cagcggctgg gccagcaagc gcttcttgtc taaagccggt acgtcttcgg
     3181 gaaggacgag ctgcccatag agtgtttcgg cagcgtcgtc cagaaaggct tcgtcggccc
     3241 tgacgatatc ggccaggcgg ttcaggtccg ccgtaatggc cgggttataa gcctgcagct
     3301 gcggcagcag gtccaggcgt atccggttgc ggctgtagcg ccggctgtca ttggtttcgt
     3361 cgtgacagac cggaaggtcc cgggccgcgg cataggctgc cagctccgcc ttggtcacca
     3421 taagcagcgg ccggatgatg tcgccccgcc tgggcgccat gccgcagagg ccgtcgaggc
     3481 cgctgccgcg caggagatgg agcaataccg tttcggcctg atcatcggcg tgatgggcca
     3541 cggcaatgcg ttcggccccg caggcccggg ccgtttcccg caggatgcgg tagcgttcgc
     3601 gccggcctac ggcttcttcc gacgcctttt cccgccgggc cagggccggc acgtcgacgt
     3661 actgccaggc aaaaccgcaa tggcggcgca cggcttcgcg ccgtaccagg ctgacttctt
     3721 catcggccgc ccggcggatg ccgtggtgga cgtagcaggc cgtcaaggtc agcctatata
     3781 acggggccag gcggctcagt atatccagca gagccagcga atcgggcccg ccggagcagg
     3841 cgacgaccag ggccctcccc ggctgccaca ggccgtgctg cctgccgtaa gcgtctacgc
     3901 ggtcgacaag gttcacgact ggcggtcccg ggagttccag gccgcatcat ggaaggaatg
     3961 gcggaaaaag acgccttcca tgagatcccg ttcgctgacg gtgatggccg ggatgtgata
     4021 gcgctggaga aaggacttgg caatgagcag gccggcgacg atgatgtcgc tgcgcttggg
     4081 ctgcaggccc ggtacgtggc gccgttcttc cggcgtcatg gcccggaggc gttcgatatg
     4141 gccgcacagg gcatcatagg aaacgcgatg gccttcgact ttctctgcat cgtagacggc
     4201 cagctgcaag tccatggccg ccagcgtcgt cagcgtcccg ccgacgccga tgagcaggtc
     4261 cggctggacc tgcatgggca gccattcctg atagcagcgg ttttccagat gggcgacggc
     4321 ctgatggctc atgacttcgc cccgcgtaaa gcgcacagcc cccatggggt agctgtggga
     4381 ccagcacagg tcaccgccgc ggccgacggc gacttcactg ctgccgccgc cgatgtcgat
     4441 gacggctgcc gtcacatcgg ggctgagcga ggccgtagct ccagtgaagc tgagcgacgc
     4501 ttcttccagg cccgaaatgg cccggcagtg ccagccgaaa cgggcccgca cttctgccag
     4561 gaaggcctgg ccgtcgatag cttcgcggac ggcactggtg gcgacggcac agaccggtac
     4621 gcctgtcagc tgttcttgcc agccttccat ggccgcaaag gacgcttcca tccctgccgg
     4681 ggacacaggc tgtgtatcat ccttatcttt ccccagcggc ctcgtcgcca gttctttcgg
     4741 cgaaaaaatc cacgtgcccg ggacttcctg ccagcccgtc atgagtcgca gggagttgga
     4801 gccgatgtcg atgactgcat acttctgata ctccatatct gctccttcct gtaaacgtaa
     4861 aaaggcgctg tctttgtgca acagcgccct tcttttttac cttccgctat tcaatacgca
     4921 ctcaatcagc atggcgcgca ccgcggccgc cgcgtttcga ttccgtattg cgcttcaaat
     4981 ccatcaggcg ttcatcgctt tccttcagga atttgctgag ccggtcttca aaagacagcg
     5041 agcccgacga acggcgcgaa tcaaagccgt tatgctggcg aaattcatgt ttctgccgaa
     5101 aatcgcgttt cggacggaat tctttctttt ccggcgccgg ctgggtctgt ttgatagaca
     5161 gggcgatttt tcccttatcg tctacagaca acactttgac gttgaccttg tctcgaactt
     5221 tcaaaaaatc atttacatct ttgacatact cattagcgac ttcagaaata tgtacaagac
     5281 ctaccttctt atccggaagc tctacgaatg cgccaaattt tgtgattcct gtaacggtac
     5341 cttccatcac actgcctact tcgattgtca tgctgttttg atttcctcct tgtaatatca
     5401 gcattactgc taaacgtata aagccattat actcataaat gccaaaaaat tcaatcatca
     5461 aaatacaaaa tatagggaat cacggcttat agggaatttc atcgggcttg gccaggccga
     5521 actgttcgcg cgcttcccgg gcgatttcat cgggatcatt cagcttgtca cgccgttcgc
     5581 gaaggcgaag attttcgtcc cgcaaggtct gttcctgctg cagggtccgg ttcatgtcgt
     5641 catggatttc ccacagcctg tagaggcgga cgccgatgag atagaagccg gcgacgatga
     5701 tggccacgag aataagatta taccagctca tctgccggag ccggatgagc agccctgtac
     5761 ggcgcttccg ggggaccttg cggcgcttct ttcggcgccg aggctggcga tactgcttgg
     5821 tttccaccca atcacccttt ctaagaacaa gagactgaaa aagtcttacc ctatagtata
     5881 ccgtgctttg ctggcaagat aaagtttttt tgtaaaattc cttttaaaat gggcggcagt
     5941 tatatataat agaggcacga cattttgtat cttatccgag ggggaacacg tatgcactat
     6001 gaaaaaataa cgacagaaat cctggaaaaa ttaaaggcca tcgtcggccc gaaagacgtc
     6061 gtcaccgacg ccgacgccat gcagccttat tcccatgacg aagtgaccga cccggcttac
     6121 catcacatgc ccgaagccgt cgtctttgcc gaaacagccc agcaggtcag cgccatcatc
     6181 aagctggcca atgcctgcca cttccctgtc gtcccccgcg gggccggcac gggtctggcc
     6241 tgcggcgccg tcccggaata cggcggcatc gtcttgtcgc tggaaaagat gaacaagata
     6301 ctggaaatca atgaagacgg cctctatgcc gtcgtcgaac cgggtgtacg cacgtcggac
     6361 ctgcaggaag cggccgaaaa gaaaggtctc ttctacgccg gcgatccgtg cagcggcgac
     6421 tcgtgcttca tcggcggcaa catcgccacc aacgccggcg gcaaccgggc cgtcaaatac
     6481 gggacgacgc gccaccaggt ctacagcgtg gaagtcgtca acccgacagg gcgcatcgtc
     6541 aacctcggcg cgcgcctgca gaaacagtcg acaggctatt ccctggacca gctggtcatc
     6601 ggctccgaag ggaccctggg catcatcacc aaggcgacgc tgaagctcct gcccaagccg
     6661 aagtacgccc tcgacctgct ggccatcttc gacgaccgcc gccaggccat gggcgtcgtc
     6721 accaaaatca tcaaggccgg catcacgccg acgtgcgtcg aattcatgga caacatcacc
     6781 atcaagtccg tcgaaaaata cctcaatgaa aatctccagg gcagcgacaa cggcaactac
     6841 gtcatcgtcg aagtcgaagg gaccagcgaa gacgacctgg acgacaaggc cgtcatcctc
     6901 gacgaagtct gcacggaaaa cggcgcgtcg tcagtcctcg tcgctgacca caataagatc
     6961 tggcaggccc gcaaggcctt tgccgaagcc gtccgcgccg aaagcctcat cgtctgcaaa
     7021 gaagacgtcg tcgttcctgt cgaccaggaa gcggccctcc tcgacgaaat cctgcgcctg
     7081 gccaagaaat acaacctggt cacacgtatc gccagccacg ccggcgacgg caacattcac
     7141 ttgaacatcc tcaagaatga agatgccacc tatgaagact gggaccagcg ggtcaaggcc
     7201 aaccagcagg aactgtacaa attcatttat gcccacggcg gccgcctgtc cggcgaacac
     7261 ggcatcggct acaagcgcaa gcatttgatg gaagaattca ccgatcccgg cgaactggaa
     7321 atgatgaagg ccatcaagaa agccctcgac ccgaacgaca tcctcaaccc ggggaaaatc
     7381 ttcgacgtcg actaacttgc aaaaatgaaa gttaccgtgt ataatacata acagaactga
     7441 ataaaacgta tatcttcagg gcagggtgaa attcccgatc ggcggtatag tccgcgaacg
     7501 ctacggcgta ggagccattg gctcgtgatt tggtgcgatt ccaaaaccga cagtatagtc
     7561 tggatggaag aagatgtggc gtgctgtttc tgcagcgtgc ctttccttgc atgtcaaagc
     7621 cctgtgcctc atcgccaggg ctttttgagt accaccaact atggacaata cagactttaa
     7681 ggaggtcttt attttgacta cagaaaaacg taaattcaac actatcgaag aagcattgga
     7741 agaattacag gccggccacg tcatcatcgt caccgatgac ccggaccgcg aaaacgaagg
     7801 cgacttcatc tgcgccgccc agtactgcac gacagaaaac atgaacttca tggccagcca
     7861 cggcaagggc ctgatctgca tgcccatgag catcgaatat gcccataagc tcggcctgcc
     7921 ccagatggtc agcgaaaata cagataacca cgaaacggcc ttcactgttt ccatcgacca
     7981 cgtcgacacg acgacgggca tctcggctgt cgaacgcgcc ctgacggccc gcaaatgcgc
     8041 tgaagaatcg gcccgtcccg aagacttccg ccgtcctggc cacaacttcc ccctcgtcgc
     8101 caaacgcggc ggcgtcctga cccgcaacgg ccacacggaa gccacggtcg acctcatgcg
     8161 cctggccgga ctcaaacagg tcggcctgtg ctgcgaaatt atggcagacg acggcacgat
     8221 gatgcgcacg ccggacctct ggaaaatcgc cgacgaatac ggcctgaaat tcatcaccat
     8281 ccacgatctc cagaactact gccgccgcca tgacaaacac gtcatccgcg aagccgtcgc
     8341 caagatgccg acgaaatacg gcgatttcaa gatctacggc tacatcaacg acctgacggg
     8401 cgaacatcac gtcgctctgg tcaaaggcga aatcggcgac ggcaagaacc tcttgtgccg
     8461 cgtccattct gaatgcctta caggcgacgt cttcggctcc cgccgctgtg actgcggtga
     8521 acagctggcc gctgccctga cacagatcaa cgccgaaggc cggggcattc tcctctacat
     8581 gcgccaggaa ggccgtggca tcggcctcat caataaactc aaagcctatg tcctccagga
     8641 acagggctac gatacagtcg aagccaacga aaaactcggt ttcgctccgg accttcgcga
     8701 atactggatc ggtgcccaga ttctccagga catgggcgct aaagaaatcc gcctgctcac
     8761 gaacaacccg gaaaaagtct acggcctcga aggcttcggc gtcgaaatcg tagaacgcgt
     8821 ccccatcgaa atgaaacccc agaaattcga cgaattctac ttggaaacga aaaagaataa
     8881 aatgggccac atcctccccc atctgcatta aaagcgagta aaaaaggcgc tgtcgcatga
     8941 agacagcgcc ttttttagca gtggctcgtg gctcgtggtt tgtaagagcc gtcctgaaag
     9001 cctttctggc ccaccaggta ccgatagggt agctgccaga atggacagct tagctggcgg
     9061 cccgccgatt tccttaatgt tcctgccgca aggtgaattt gtccttgtgg taatcgatga
     9121 ggccttcttt tttcatcttg ctcagttcgg ccgataaggc gctgcggtcg acggagagga
     9181 agtcggccat ctgctggcgg ttgaaaggca ggacgaaggt ggccttccct tttttctggg
     9241 ccatgtcgga caggtagagc atgattttct ggcgcgtcga gcgctgggtc aggtagcgta
     9301 tcttttccgt aaggaggcgg ttcttttcgg ccatcagagc caggagattg cctgtaaaac
     9361 gagcctggtc cggcgtcagg acctgaccgg catggaggat gttgccgaca tagacctgca
     9421 tgatgaccgc atccgaggcg gcgacgacgt cgaccgtcgc cggcgcgtca ggcagacagg
     9481 caaaggcttc gccgaaaaag aagcctgccg ggatctggga caggatattg cggttccccc
     9541 agtaatcttc ctggatgatg tggaccgccc cttccaggac cagtccgata taggggacgg
     9601 catcgccgga ataaaagagg aattcccctt tctcatattt tttcttgcgt accttcaagc
     9661 gctgcaagag cggcaggaca tcggcttcgg cgacgccgtg gaagagcggc gacgtttcaa
     9721 tgagggaaag atattgttcc atggaaagac ctccaaaaaa aagatgatag ttggaaaaac
     9781 aacagcttat ttttgctcca tcgactatac ttagaccata aaggtcccaa accagtttga
     9841 tgcatatacc acgatggagg aaattatgat acgtaaaatt atacagattg attcgaaaaa
     9901 atgcaatggc tgcggcgcct gcgccacggc ctgtcacgaa ggggctatcg ccatggtcga
     9961 cggcaaggcc cgcctcatgc gcgatgacta ctgcgacggc ctgggcgact gcctgcccca
    10021 ttgcccgacg gatgccatca cctttgtcga acgcgaagcc gctgcctatg atgaagaagc
    10081 cgtgaaacag cacatggccg cccagggccg ggcctacacc ggccgctgtc gcggcagcca
    10141 ggcccgcagc ctcggcgaaa cgcagaaaag ggctgacgac gttcctgccg ggccgtccat
    10201 ctcccgcctg agccagtggc cggtccagct caagctcgta ccaccccata cgccttattt
    10261 caacggtgcc agcctgctca tcgcggccga ctgtacggct tatgcctacg cccgcttcca
    10321 cgaagatttc atcaagggcc gcatcaccct catcggctgc cctaaactcg acgacatcga
    10381 ctatgccgaa aaattgacag aaatcctccg tcagaacgac atccacgacg tcaccctggt
    10441 ccgcatggaa gtcccgtgct gcggcggtct ggaaatggct gccaaaagag ccctcgcaca
    10501 gagcggcaaa gatatcccct tcgaggtacg catcatcagc ctcgacggga agataataaa
    10561 aggatagtaa aaaggcttgc cgcgtagcgg caagcctttt tatcgtaggg gccgtcccaa
    10621 agggcggccc gttctggtta gttgttagcg gttcgtcaat gtctgcgtga cgcccttggg
    10681 gcatccccta cgaactacga ggcacgacgc tctttacaca ttccattctt ccttcagtgt
    10741 cgacaggcac atgaaggtct gggacgaggc gaccgtcggg atttcgttca atacctgcat
    10801 gaggaagtgt tccaggtcat ccggtgtctt gaccagcact ttgagcaagt agtcaaaggc
    10861 gccgacgacg tggtgacatt ccaggacttc cgggtactgg acgattttcg agcggaattc
    10921 ttcgacatcg ccagagcgtt cgagattgac catgatgaaa gccgtcacgt tgtaaccggt
    10981 cttgcggcgg ctgattttgg caccgtagcc ttcgatgatg ccgttgtgtt ccatcttgcg
    11041 gatgcgttcg gcaacagccg gaatggacag gccgacgacc ttgctgattt ccgtagccga
    11101 agctttgcca ttgtgtttga gtatcgttaa aattttgtca tcgatatgat ccatatgtag
    11161 atactaccct ccataatttc taaaatttac atatctatta tcttacaaac gaatgaaaaa
    11221 gtaaatagac ttaaagatat taattttttt tctgttatac tttttgcttc tatggagact
    11281 ggtttattcc gaatcggaaa gatggaaatc actattcaag tcagagaaat tatcggcaaa
    11341 gccctgggtc atgtagacgg cattgtcgct gcctgtgatt tcacagttga cgccttccat
    11401 gctgcttgcc aattccatcc ccttctgcgg tcccaggacg aagacgatag tcgtcatcat
    11461 gtcgctgagc atgcctgcat gaggtacgga accgtcgatg acgacagagt cgctcatgac
    11521 gccggaccgg gcgggatagc ccgtgcgggg gtcgaagata tggtggtagc ggacgccatc
    11581 ctggatgaaa tagcgttcat agtcgcctga tgtggacagg gcctggttct cgatggatac
    11641 gatgcccagg aagctcttgt cgtcgtcaga gcggggatgt ttgataccaa tgcgccaggg
    11701 cttgcccttg gtcgtcttgc cgacggcata catcgagctg gcgccgaggt tgatgaggcc
    11761 gctttcgatg tggtgttttt cataaatctt gcggatttca tcgacagcat agcctttggc
    11821 cacgccgccc aggtcgatgg ccatgcccgg tttagccagc atgacgctgt ggtcttccgg
    11881 ccgcaggagg atgtccttat agttgacctt catcagggcc tgggaaatct catcggctga
    11941 cggcacgtgc tggtcgtcgt tgccgatgcg ccataaattg gtcatgacgc caacggtaat
    12001 gtcccattcg ccctggctct tggccgaata atccttggcg tattcaatca tttcataaat
    12061 ggccgggtcg acctggacgt atgctttgcc ggcagcggca ttgatacggc ttacgtcgct
    12121 gtcttcattc tgtcctgcca gtttgtccag ctgattcagg cgttcaaagc cttcatcgac
    12181 tgcttctttg gcttctttcc cggacgcccg cagggagacc gtcgtatcca tggcgacacc
    12241 gctcctatca aaagaagact ccggatgcag actgcatccg gagcaactaa ggatgatgag
    12301 gaacgccatg gtgagaagaa cccatacata ccgtttctta tccatgtatt aacctacgtt
    12361 ttcttctttc ttgaaagagc ctttgatcat ttcgttgatg gcaccagtcg ggcacttggt
    12421 caggcaggta gcattgtcac aggtatggca gatgtcctgg ttgacgacgg ccagatggtc
    12481 gatgatcttg atagcccctt cttcctgatg cgagcagtta cgcatgcagg aaccgcagcc
    12541 gaggcacagg gccttgcagt tcttcatagc cggagcgccg aaatctttgg agctgcagct
    12601 gatgctgact ttggcactga tcggcaggag ttcgatgacg tggttcgggc agacagaagc
    12661 gcatttaccg cagcctgtgc agagcgtctt gttgacgaca ggcaggccat tttcgcccat
    12721 gctcatagca ccgaacgggc aggcacgcgt gcagttgccg aagccgagac agccgaattt
    12781 acagaatttc gggccctgct ggacgatctt agcagccgcg cagtcatgaa cacctttgta
    12841 ttcataagcc agtttggcaa tgccattgcc gccgcggcat ttgatgtgag cgtatttcgg
    12901 ttccgtagct tcagcgactt tgcctgtcaa cttagcgacg acagcagcga cttcagcttt
    12961 accaggaaca cagaggttag gcggaacgtt cgggtcgagg acgacggctt ccgcatattt
    13021 tgcacagccg gcgaaaccgc aggcaccgca ctggccctga ggaagtgctt cttttacttc
    13081 gccgatgagc ggattttctt ccatggcaaa tttcttatca gcacttgcaa ggataaagcc
    13141 gaagacgata ccgatgcaaa ccatgacgcc gacagcatac atagctatcg ttgtataatc
    13201 gtacataatc taattcactc ctttgcctta aatcataccg gagaagccga ggaaggccag
    13261 ggacatcata ccggcgacga tgaaagcgat tggtgtgcct ttcagagcgt ggggaacagc
    13321 ggcatagtcc aatttttcac ggcagcttgc catgaggatc agagctacgg cgaagccgag
    13381 gcctgcgcca acagcgaaga cgatgttttc caagaagtta taaccgctgg tgacactgat
    13441 gaccgggacg gacaggacga tacagttcgt agcgatcagc atgaggtaaa tgccccacat
    13501 gttatacaag gtcggcgatt ctttcttgat gacgatttcc agtaactgta cgaaacttgc
    13561 gatcaagata acgaaggaaa tcgtgcgcag gaattccaaa tgaagcggta ccaggactaa
    13621 gttgtacagg caccatgcca ggatagtact catagtgatg acggcagtga cggcgaagcc
    13681 catgccgaca gaggcgttca ggttcttgga aacgccgaag aagatacaca aaccgaggaa
    13741 cttggtcaat acgaagttgt taatcaagac agccccgatg aacagcgtaa agtattccat
    13801 tatccttcac tccctttcag ttgtctctgt ctttcaacat gttcattcca gattttgaaa
    13861 ccagccatga tgaaaccgat gagcatgaag gctccaggcg gcaaggccat gatcagagcc
    13921 ggctgatagg acggaccgaa gacagggata ccaaagaagg taccgttgcc gaggatttca
    13981 cggacgaagg aaatgaggaa catagcaatg gtgaagccaa tgcccatgcc catgccgtcg
    14041 aggaaggaaa cgacgacgcc gtttttcgat gcaaccgatt cagcacgagc caggatgata
    14101 gcaaatacga cgaccaggtc gaggtatacg cccagggctt tgtacagatc cggagcaaaa
    14161 gcctgtaaga acagctgtac gcacgttacg atagtagcga tacaggtgat gtaaacaggg
    14221 atacggactt ttttgttgat cagcttgtga atgatggaaa cgaccgtatt gttagccgtg
    14281 atgacgaacc atactgtgca gcccatggtc aggccattga cgatgcttgt cgtaacggca
    14341 aggcccgggc acaggctcaa ggccaggacg aagtacgggt tttcttcaat aatgcctcta
    14401 gtaaatactt tccaaagatt cattattatt taccccctaa ctctgctgct ttctgaacag
    14461 cctgtttaac gcccaaagtg aaagcacggg aagaaatggt agcaccggtc atagcctgga
    14521 ttttatcctt ttcgctagca ctcttggtga cttccaagtt ttccagtttt ttgcctttga
    14581 actgatcttt aaacggagat ttagcacctc tgtcgccgag gcccggagtt tcgttggctt
    14641 cgaggaccgt gtagtccata accgtgccat ccatggaaac agccgtcagc atcttgatag
    14701 cgccgccgta acctttcgga gccgtcggaa tgatataagc cactttcttg ccgccctgtt
    14761 cagcgacgaa ggtatctttt tcgccaggga cgggatcgaa tttttcagcc tgcggaacga
    14821 gggatttcat ggaatcctgt ttcatctgtt cagctttttc ataggcaatc ttgctggta
//
LOCUS       DAWCYS010000016        14829 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_270_length_14829_cov_7.796128, whole genome shotgun sequence.
ACCESSION   DAWCYS010000016 DAWCYS010000000
VERSION     DAWCYS010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14829)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14829
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_14829_cov_7.796128"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3208 a         4111 c         4264 g         3246 t
ORIGIN      
        1 cggcacagga cgcgggaccg gatgaagccg caggcgatga tgaaggccct ggccgtgccg
       61 acgatgaaca ggagaagcat ttgcatgacg cccttggcca tgccttcgcg gatgccgctt
      121 ttagcaatga acggcgtatt gccggcagcg atgaccgtcg tcccgctgtg gggggacgtg
      181 tagcggaaaa agaaaccgtc gccgtcccag gtcatgaccg tctgctggcc atgggctttt
      241 ttcaggaccc tgtcggccag accataaaag tcgatgcccg gcgtgatata ggcgacctgg
      301 tcgttgcgga cgatggccgt ggcaatgccc aggtcttcca ggacccggca gtcttcgcgc
      361 atatccttga cctttaacgg cccaggccgt tcggccttga cgcgaaggga actaaccgta
      421 tactggatgg acagggccgg ccccttttcc ggccataacg tcgtcaaatc attgtgaggc
      481 gatgaaaagc ggagtcctga gatgagtagg aatcccaggg ccgtcaaaat agcgatggga
      541 acaaagacca tggtaaaatt agtgatgagc aggcggtgtc gcagtgaaat atcacgtata
      601 tgcagcataa acaggccatc ctttcgtagt gatagtttca ttatagagtg attcgtggct
      661 cgtggctagt gattcgtagg gggatgtcgc acgaaggttt ctgccatcat gcgcggctcc
      721 tttttctcta tagtttgtag catggagttt gtagtttgta gaaaactaaa aactacaaac
      781 tacaaactta aaactacaaa agaggctgtc ttcatggaca gcctcttttg tatcaagcta
      841 atcttcaatt tctgtatgga tgacggtgcc gttagtggcg tctacgagga cgtcgtagcg
      901 gacggatccg gatatgacgc ggactttata catggggtgg aaggcgcgtt gcggcatagc
      961 tccctgcggc ggctggcctt tcatatccgg ctggcccggt tgtccagaag cagtcatcgg
     1021 cggctggccc tggggcggtg tcatgggggc ggattggttg ccggcctggc cgtcgggagc
     1081 ggctttggcc gtagcaccgg cagcggcatc ggccttttct tcaccaggac ggccaggttt
     1141 catgccttct ggtccgcgct ggtcgcctct ttcatcgcct ttgcggtctc tgtccttcgc
     1201 tttttctttg tggtcgcctt tgcggtcatc cttgcggccg tctttcggac cgtgcttggc
     1261 gctgctcata tcgaagaggg cgatttcttt gaaatccaat tcactttcgt ccttgccgat
     1321 ggcctgagcg gcagcggcgc aggcctggtc ttcactgatg agggagatgt tgtgctggtc
     1381 ggcctgggcc ttggtcatct gggccatggc gtcgacccgt tcggcgtgac gggccagatg
     1441 ctgttggtgc agatagtagt ggccgccggc cgtggctacg acgcagacga cgcagaaggc
     1501 agcagcgatt tttgcattgc gggccgtgca gactttattt ttaatcgaaa caagtgtatc
     1561 ttgccatttt tcctgggtca tgatgaattc ctcctgtctt caatcactta ttcttgacta
     1621 cgaggacagt ataacggata aatctaaaat cgagctaaac caggccgtaa gaattacata
     1681 aatatagtat aatgagtttg tagcctatag tttgtagcag atggttttaa atttttagcc
     1741 atcactgaaa actaaaaact gcaaactcaa agctaacaat aaggccgtct ttgtacgatg
     1801 accttattct gaatactgta acaccaagga ggtatctacc atgaacgatg aacgtttgaa
     1861 attgactcaa atggcgtcct gctccggttg aggcgctaag ttagggccag ggacactggc
     1921 tcaattgttg gacgggcttc caacacataa agatgaccgc ctcctcgttg gctatgataa
     1981 gagcgacgac gcgtcggtct atcaggtgag cgatgatctg gctgtcgtcc aaacggtcga
     2041 tttcttcccg cctatcgtcg acgatcctta tttattcggt caaattgcgg cggccaacgc
     2101 cctgagcgac gtctatgcca tgggagccga agccaaactg gccatgaacg tcatgtgcgt
     2161 caacctgtcc atgggccgcg aagccgtccg ggctatcctt gaagggggct accagaaggc
     2221 ctatgaaggc ggtgtcatca tcaccggcgg ccatactatc gaagacaagg aaccgaaata
     2281 cggcttgtcc gtcacgggtt tcgtccatcc acagaaactg ttgcgcaact cgtcggcccg
     2341 gcctggcgat gtcctcatcc tgaccaagcc cttaggcgtg ggcatcctca tgacggcagc
     2401 ccagggcgat ttcgtggcca agccgctcct gcagcagctc tacgaccaga tggcccagct
     2461 caataaaggg gcccgcgaca tcatggtcgg ctacgaagtc cacagctgta ccgacgttac
     2521 gggcttcggc ctgctcggcc atgcctgtga aatggctgac ggcagtggaa cgacgattca
     2581 ttttcagagt gacgccattc cctaccataa acaggcattg gacatggcag ccatggggct
     2641 gatcccggct ggagcctacc gcaaccgcga ctttgctgcg ggacgcgtca aggcggacgg
     2701 cgctgataag cgcttcatgg acatcctcta cgattcccag acttcgggcg gcctgctcat
     2761 cgctgtcgct gaaaaggacg cgctggccct ggaacgggcc ttgaaagatg ccattccctg
     2821 tgcggccgtc gtcggctatg tgaccgagcg ggacaaatgg tccatcgtaa tcgaataaaa
     2881 cttgtaaaaa cggctacctt cagccccata gatagtgtat aataaagttg gtgagttttt
     2941 ttaccgtact catgtcaact acaaaggaga gatcaactgt gattgaacgc tatacgaaac
     3001 cggaaatggg tcatatttgg acagaataca atgaatggga tacgatgaaa caggtcgaaa
     3061 tcctggctag tgaagcacag gccgaactgg gtaacatccc gaaagaagcg gctaaggaaa
     3121 ttcgcgaaaa agctgctttt tctgtcgacc gcatccatga aatcgaagcg gaaacacacc
     3181 atgacattgc ggctttcgtc tcgacgcttt cggaaaatat cggcgaagcc ggtaaatatg
     3241 tacacctcgg cctgacctcg acagacgtca aagacacggc tctgggctac atgctcaaac
     3301 aggcttcgga catcctcatc aaggacctgg aaaacttcca cgccgtattg cgccgccgcg
     3361 ctgttgaatt caaatatacg ccgtgcatcg gccgtaccca cggcatccat gccgaagcga
     3421 cgacgttcgg cctcaagctg tgcaactgga tggctgaaat cgaacgcgac atcgaccgca
     3481 tgaaacacgc caaggaaacg atttccgtcg gcaagatctc cggtgctgtc ggtacctatg
     3541 ccgacattga tccctttgtt gaaaaatatg tctgcgaaca cctcggcatc gctgcttcgc
     3601 cgatttcgtc gcagaccttg cagcgcgacc gccatgctga atacgtaacg accctggctg
     3661 tcattgccag ctccatcgac aagatggcta cggaaatccg tcacctccag cgcacggaag
     3721 tccgcgaagc cgaagaatac ttcagcccca aacagaaagg ctcttccatc atgcctcaca
     3781 aacgcaatcc tatcacttgt gaacgcatgt gcggcttggc ccgcgtcatc cgcggcaatg
     3841 cccaggctgc tttggaagac gtcgccctct ggcacgaacg cgacatttcc cattcgtctg
     3901 tcgaacgcgt catcctgcca gacagcacta tcgctctgga ctacatgctc cagactttca
     3961 cccgcgtcat cgacaagctc ctggtctatc cggacaagat gatgaaggac ctcaacctca
     4021 ctggcggcct catctacagc ccgatcatcc tcaacacgct cgttgaaaaa ggcgctgtcc
     4081 gcgaacaggc ataccgctgg gttcagcgca atgccatgaa acgctggctc gaaggggaag
     4141 acttcctgga aaacctgaaa aaagacgaag acgtcacgaa atacatgaca gccgaagaaa
     4201 tcgaaggctg cttcgacgtc aagaagatgc tcagccacgt cgacaccatc ttcgcacgtt
     4261 tcggaattta aaaatagttt gtaggggctc gcacgtggcg agcccgcccc gaggatatgt
     4321 aaaaaagggt ttgtcgtcgt gcggcaagcc cttttttgct gtctcgaaaa tctgcatcca
     4381 cgaaagcctc tcctaacagg agagccgggc cgccctttgg gcggccccta cgaaccacga
     4441 gccacgaatc tcccaggagg tgattttatg tattatttcg ataatgcggc gacgacggcg
     4501 cagaagcccg aggccgtcgc ccgggccgtt tacaatgtcc tgtcatcggg aaccgtgggc
     4561 aacccgtccc gcggggccca tggctacgcc ctgaaggcct atggtatcgt cttgcaggcc
     4621 aaggacgatg taaaggcctt gttccactgc ggcccggaat atgatgtcgc ctttacgcat
     4681 aactcgacgg cagccctgaa catggtcctc aaagggctca tccatcccgg cgacggcgtt
     4741 ttgacgacgt cgtgggaaca caatgccgtc ctgcggccgc tttaccagtt ggaagcccaa
     4801 ggcgtatccc tggctttcat cgccagcgac agtcccggcg gcgccttgca gtatgacacc
     4861 ctggaagaac ggctgacacc gcggacgaaa tggttcgtct gcaaccacgc gtcgaacgtg
     4921 accggcaatg ttttggacct gccccgcatc aaggccttct gccagcgtca ccatctgggg
     4981 ctcatcgtcg atgtatccca gacagccggc gccatcgacg tcgacttatc cgatggcatc
     5041 gtcacggcag cctgctttac gggccataag tcgctttacg ggccaggcgg gacgggcggc
     5101 atcgtcatcc gccgcgatgc tctcgtgacg ccgtacatca ccggcggcga cggcgtccat
     5161 tccttcgagc gggagcagcc gtcggctgtc cccggtgtct ttgaagcggg tacggccaac
     5221 gtagcgggca ttgccggcct cgacgccggg ataaagcagc tcctggatgg tggcgtcgcc
     5281 gctgcggctg ctcatcagga agcactggcc cggattttcg tacccgccgt ccaggctatc
     5341 ccgggtatcc gcctgtatgg cgacttttcc ggaccccgcg tcggcgtcta cagcctcaat
     5401 atcggcgatg cagaatcggc cgtcgtcagc gacatccttt ggcagacata ccagatggcg
     5461 acgcgcccgg cctatcactg tgcgcccctc atccatcagg ccctggggac ggccgtccag
     5521 ggagcggtac gcttttcttt ttcccagttc acgacggctg acgacgtccg ggccgctgtc
     5581 cgggccttgc gggccattgc ggcgatgtaa gattttctgg caatccatca ggaaaacttg
     5641 actgctttgg tatagtaata tataatagat atagttataa aagacaaata gtcaaaagaa
     5701 aggtggaatc tctcatggaa tataaattca cagccgataa ttttcagcag gaagtattga
     5761 acagcagcca gccggtcatg gtcgactttt tcgctaactg gtgcggcccg tgccagatga
     5821 tgggaccggt catcgccaat ctggccgaag aatatgccgg caagattaag atcggcaaga
     5881 tcaatgtcga cgaaaatccg ggcgtagccc aggcctatga agtcgccacg atcccgaacc
     5941 tggtcttctt caaagacggg aaagtggtca accgcgtcat cggtgtcgtc ccggacaatg
     6001 tattgaaaga aaaattagac gctttgctca aataagcagc tggcgccgga ggaggatttt
     6061 catgaaaaat tattcctata tgacccaggg gacttgtgcc cgtcagatta ccttcagcct
     6121 agaagacggc aaactgcatg acgtccattt cgtaggcggc tgtcccggca ataccctggc
     6181 tctcagcaag ttgttggaag ataccgatgc cgcccgggcc gtaaagctcc tgaaagggaa
     6241 tccctgcggt gaccgcggta cctcctgtgc cgatcaactg gccatcgccg tcgaacaggc
     6301 cttgaaacaa ggcgcctgat ggcggagtca gtctatggcg tcgccctggt gccgtcgaca
     6361 cggcaggcta tggaaggcga aaagctggcc aaggcagccg gcctggccgt gcgcatcatt
     6421 ccgacgccgg gaaagctcga cgcgtcgtgc ggcttttccc tgcgctatga actggttgat
     6481 gaagcggccc tgacagctct cctcgatgag gaggggctgg catgggtcgc tttatatcat
     6541 gcctgccgcc agggcctgac cgtcacgtac actaaaatca gagaagggtg atattatggc
     6601 cagacatatc gtcatcggga cggccggcca tgtcgatcat ggcaagacga cgctggtccg
     6661 ggccttgacc gggatcgata tggatacgac agcggaagaa aagaaacggg gattgaccat
     6721 caacctgggc tttgcctggc tcgacctgcc tgacggtacg cgggccggca tcgtcgatgt
     6781 accggggcat gaaaagttca tcaagaatat ggtcgccggc ctgccgggcc tcaatctggt
     6841 cctgctcgtc gtcgatgcca acgaaggggt catgccgcag acgaaagaac atctcgacat
     6901 cttgaccctt cttggcgtac ataaattcat catcgtcgtc accaaggccg acaccgtcga
     6961 cgacatgctg ctcagcctga ccctgcagga catccgccag aatctggccg atacggcagc
     7021 cgaaggggcc ccggtcgtcg tcaccgatgc cgtcagcggc cgcggcctga cggaactggt
     7081 cgatgaaatc caggccatgg cagcgacgct gcccatggaa agcgctcagg ggatgggccg
     7141 tctcaacgtc gaccggtcgt tctccatcaa gggattcggg actatcgtca cggggaccct
     7201 catcgacggc cccatgatga atggccgcga agtctggctt tatcccggcg gccggcgcct
     7261 gcgcatccgc aatatgcagg tccacgagca ggatgtcctg cgggccgacc cgccgcagcg
     7321 gacggcattg aacctggcag gcatttcctg tggcgccatc catcgcggtg acgtcctctg
     7381 tacggcgccg gattttacag cgacgcggat gatcgatgtc aaagtgacct gcctggctga
     7441 cgcgcagccc ctttttctat ggcagcgcat gcgcctcctc gtcgggacgc gggaagtcat
     7501 ggcccgcatc gtacccttgg gagccgaaca gattgcgccg gggacggagg gctttttgca
     7561 gctgcgcctg gagcaggatg aaatctatgt caaggctggg gaccgtttca tcctgcggac
     7621 cttttcgccc atgcataccg tagccggcgg ggaaatcctc gacgcccatc cgcagaaaca
     7681 ccgccgcttc aaagacgacg tcgtgaccag cctggaagcc cgcgatgccg gtctgacgga
     7741 cgacgtcgtc gccggcttcc tgcgcctgcg ccaggtcccc tttacccagg ctgacgtcat
     7801 tgccggggcg acggacctgc ccctggataa ggtagagctg gccctggacc atttgcggca
     7861 aaatgggatt gtccgccgga cccgtcaggg ctacatccac ggtgacgtct ataagacctg
     7921 gcaggccaag gccctgcaag tcctcctggc ctatcataga gagaaaccgc tgcagcctgg
     7981 gattccccag ccggccttcc ggagccgcct gcatctcgat gaaaatgacg ggacggccct
     8041 attgcggctg ctgacggctg gcggcatctg ccgcctgtcc cgccagtgcg tagcggcgaa
     8101 acaattccgt atcacctttt ctccgtctca gcggaaattg cagtcggcta ttgaaaagaa
     8161 gctggaccac agtggctatg tgcccgtccc ggtccgcgac gtccgggctt tgggccggga
     8221 agcccaggcc gtcgccgatg ccctcaacgg caagagcctc gtcttcttat cgcctgattt
     8281 catcttgtcg aagcgctaca tgatgaaagc cgctgaagcg gcctgccggt atctgcaatc
     8341 aggctgtccg ctgacactcg gcgattttcg cgatgtcctg ggagtcagcc gccagcaggc
     8401 cctgctcatc ctggaatata tggaccgctg tggcattacc cgccgcgttg ccgacgggcg
     8461 cctggccgga ccggcagccc ggaaatatga aataaaaggg gagaatgacc atggatgaag
     8521 aacgacagca gctgctccgt cagctgcccg atgtagacca tttactgcaa cggccggtac
     8581 tgctgacctg gcaggagagg aaatcgcgcc aggctgtccg cgatgccgtc cggaacgtat
     8641 tggatgaacg gcgtcaggcc atcttggccg gccagtggga ccagcctttt tccggcgatg
     8701 actttgaccg ggccgtcgtc tccctcctgc aggcagaaga aacgggccat ctgcgccggg
     8761 tcatcaacgc caccgggacg gtcctgcata cgaatctggg ccgggctgtt ttcagtccgg
     8821 acctggcccg tcacattgcc aatctggtct gccattacag caatttagaa tacgacctgg
     8881 cttccggcaa gcggggatcg cgctatgacc atgtccggga acttctctgt gagctgaccg
     8941 gtgccgaaga tgccatcgtc gtcaataaca atgccgctgc cgtcctcttg accctgtcca
     9001 gcatggccgc cggccgggaa gtcgtcgtgt cgcgggggga actcgtcgaa atcggcggct
     9061 ccttccggat tcccgacgtc atcgaacaga gcggcggccg tatctgcgaa gtcggtacga
     9121 ccaacaagac tcatttgtcc gactacgaac gggccatcgg cgaagatacg gcggccctca
     9181 tgaaggtcca caccagtaat taccgcatca tcggttttac cgagagtgtc tccgctttgg
     9241 acctggcccg cctggcccat gaccacggac tgccctgcat caacgatgtc ggcagcggcc
     9301 tgttcgtcga tttggccgca tggggactgc ctcatgaacc gacggtccgc gaagtgttgg
     9361 acgctggctg cgatgtcgtg actttcagcg gcgacaagct cctgggcggc cctcaggcgg
     9421 gtatcatcgt cggtaaagcc gcctatctgg atgccatgaa acaccatccc ctgctgcggg
     9481 ccctgcgcgt cgataagatg accctggccg gtttgcaagg gactttgcag acctatcgcg
     9541 acggtacggc cgtttgccag atcccgacgc tgtactgcct ggcccagacg aagaaagaga
     9601 cgcgggaacg ggcggaacgg ctgttgcagg ccctgcatac agatgacctg ccggcctgcg
     9661 tttccctgcg ccgtgtagac gattgtgtcg gcggcggcgc ctgccccgat gccgtcctgc
     9721 cgggctgggc cctggccatt gatccctatg acctgtcggc ggaaaaactg gaacagcgcc
     9781 tgcgccaggc cgacatcccc atcatcgccc gcgtccagca ctgcgccgtc gtcctgtccc
     9841 tgcgcaccat cggcgacgac gaattcccgg acgtcgcccg catcgtgcgg gaagcgttgg
     9901 gcgtttgagc gtcgcgtgga ggtttctgcc atcatgtatg agcgggccgt tctttatagt
     9961 ttgtagtacg gagtttgtag tttgtagaag agggttttga attaaaaaaa cttcactaca
    10021 aactgcaaac ttaatactca aaaaggcgct gtcttcatat gacagcgcct ttttacattt
    10081 cctaccgcgg ggatgattgg ccgatgattt cttttccttc tacgaactgg accgtaggat
    10141 tcggtttttt cttttcaatg aggtcgacca tgaggtgcag acagtgctga ccgatttcgc
    10201 ccatggggat atcgacgcgg gacagggagg gaatggcgta atcattgaat tgagccatgt
    10261 tgatcccgat ggtgaagact tccacgtcct gtgggatagc gatacccagt tcatggcagg
    10321 tatagagcat gccgtgggcc aggacgtcgg attcacagaa catagcccgg ggcatccgct
    10381 tttcttcata gagcttcttc gtagcttcca cagcgctcga tgaagacgta tcgcagagga
    10441 tgatgttttc ttctggtacg gcgtggccgg ccttggcgaa ggtgtcgagg aagccctgca
    10501 tccacgtgcg catgatcggg aacgacgtgt catgggtcag gacgccggcg tcgaagatgc
    10561 ccttgtccag gaagtgctgg gcgacgttgc agccgatagt atagttgtcc atggtgacgc
    10621 tgctgtattt atccgattcc cggttaaaga ggacgacagg gcaggggata tggatggaat
    10681 tgaggtagtc catatcttta ttggacatat tcgcgatgat gatgccgttg aaaaattcat
    10741 tctgcatggc ttcgagctgt ttttgcagct ggtcgacctt ataagggacg acggtcagat
    10801 gaatggcttt gtcctgtttc atgatttctt cctggatgcc gagggtgatg cgggccaggt
    10861 agttgatacg gaagtccgtt gcccagtaga aggcaatggc atagctgtcc tttttagccg
    10921 accgcagttt tttggacgac tggttgggga cgtaattcag gtcttgtgca attttgtata
    10981 tacgctgttg tgtttcctgc gagattttcc gttcccggcc cttgccgttc aggacgatgg
    11041 aaacggtggc gatggaaacg ccagcagctt tggcaatgtc tttaattgtg gacacgatgg
    11101 ccacctccac tcttgctttt attttgacat aaattaaacg ttttttcaag gaaatctgtg
    11161 ttacaggatg aacaaatatt catttttatg taagtcgcga atcgtttgca atcatggtac
    11221 aatagaagcg attaagacga gaaaagagga tgtgtatgac agtgagcaaa gaacgttacc
    11281 ggcgccaggc ccttcatcaa atcgacaatc tggccaagcc gccgggaagc ctgggacttt
    11341 tggaaaagca ggccaagcag attttattgg cctgggggac ctttcaccgg gagctgcggc
    11401 cgaaacacat catctttgcc gccgacaatg gtgtcgtccg ggccggtgtc gtcgcgcagt
    11461 tgtcggatat cacgtatatg cagagctgcc atatggtaca ggggacgtcg gccgtgacct
    11521 gtttctgccg cagccaggat atcccttatg aagtcgtcga tgtcggtatc gacagtgatg
    11581 atgccgtcgg actggaccgc aagatcgcca gggggacaaa gaactttgcc gtcgaaccgg
    11641 ctatgagccg ggaagagctg gcgcaagcca tggcgacggg ccgcgaacgg gtgcaggcag
    11701 cggccaaaga tggcatcaat ctcctgtcat tcggggaaat gggcattggc aacacgacga
    11761 cgtctgccgc ggtccttaca gcattgtcgc cgaaaaagat gtcctggctg actggcttcg
    11821 gttcggcccg gggcaattat aaactgctct tccataagcg ccatgtcatc gccgcagccg
    11881 tcaaggccta tgaaaaagaa atccagatgc cggctgatgc cctgcgctgc gtcggcggct
    11941 ttgatttggc agcactctgc ggcgccatga tggcctgtgc cgacgaaaag attccctttt
    12001 atatcgacgg cttcattacg gcgacagccc tggcctgtgc catcgccatg aagcctgccg
    12061 tccgcgatta tgccctgccg tcccatattt cccgggagcc cggcatggcc caggccctgc
    12121 gcctgtgcgg catcgatgaa tacgacgtgc ccatccaggc cggtctctcc ctgggtgaag
    12181 ggacgggggc cgtcttgggc gtcgtcctca tgaagaccat gatgtatgcc gtctggcata
    12241 tggctaccct ggacggcatc aaccaggaag cccaggaccg ccatgacaac cgcaaaggag
    12301 atgaaacgca tggatgaaga aacgagaaaa aaagcccagg ccatctttga gcgcctggga
    12361 ctgacggaag aacaagcagt tgaactcttt tataaacgga ccattgcggc cgggaaaatg
    12421 ccctttgctg cagccgaagg ggaaaccaag aaaatcaaga aaaaacgcat gaacgaacgc
    12481 ttcgccgaat gggatgcttt ttagcggacc gcagaagctc aattcgtgca gccatctccg
    12541 cttgtctttg ccgggctgac tgcgtcgaaa agcctcgaca gagccccgct atgcctgcgt
    12601 ttttctcctt gccagcccgg caaatccggc gcggatctga ccgcgtcatt gagctcctgc
    12661 ggtccgaaag gctctcctgc gaaggggagc tggccgccga aggcggactg aggggttctt
    12721 aaatgtacga gccacgaacc atgaaccact ttaaattatt tttacgaaag aagggatttt
    12781 gatgatgtat ccgttattac atgcaacgct gaatgttggg tctctggttt tgaagaaccg
    12841 tctggtcctg ccgccgatgg cgacggaaaa gtcggcgaaa ggccaggtga cggatgggct
    12901 ggtcgcttat tatggtgata tggcccgcag cggtccaggc ctgatcattc aggaacacag
    12961 tttcgtttcg cctgaaggcc gggccagtgc caaccaggtg tcgctggcgg ccgatgccga
    13021 tattcccgga ctgcagcgcc tgacggcggc tgtccacgct cagggcgtac ccatcctgac
    13081 acagatcagc catgccggca gtgctgccca tcgggccatt acggggcagg aagtcatcag
    13141 tgccagtgct gtttccaatc cgtcgcgggc cgcttcggtc cagggccagc cggaatttcc
    13201 ccgggaaatg acgcagggcg atatccagcg catcgtccag gcctttgtcg acgccgctgt
    13261 ccgggcacag caggccggct acgacggcgt ggaaatccac tcggcccatg gctatctgct
    13321 ggaccagttc tattcaccgg tgaccaataa gcggaccgat gactatacgg gccagacctt
    13381 ggctggccgg acccggctgc acgttgaaat cattcaggct atccgccgcc aggtcgggtc
    13441 tgattttgtc ctttccctgc gcctgggcgg cagcgattat atggatggcg gtgccgtcgt
    13501 cggcgatgtc agcgaagcgg ctaagatttt tgaaacggcc ggcatcgaca tgctcagcct
    13561 ttccggcggc atgagcgtct tcttccgccc tggccatacg gaagcgggat attttgccga
    13621 attatcggca gcagccaaaa aagggacgac ccttcccgtc ttgctgaccg gcggcattgc
    13681 cgacggcgcc gaagcggaag ccctcctgga aaaagggcag gccgacctca tcggcatcgg
    13741 ccgggcggca ctccgggacc accagattta ccaaaaaatc ttgtccatgt agaaaaaaag
    13801 cgctgtcgca tgaagacagc gctttttgag tattaagttt gcagtttgta gtttttagtg
    13861 aatggttttt aaatttaaag tcgtcttcta taaactacaa actttaaaga gaaaggagcc
    13921 tcgtacgatg gcagaagcct tcatgtgaca gctatgaatt atgttttaat gaatacggtc
    13981 tgtttttttc tgagcaaaaa gccattctaa ggcgggctgc atggtgtagg catgctgcca
    14041 ggtagagcgg tggctgccgc cttggtactg ggtcaggtag tggctgcagt ccggcgtgat
    14101 gagggcagcg gcctgggcgt cgacctggtc ttgcggctgg gtggggtcga gggtctggct
    14161 ggctactttg acgccgtctt tttctaagtt ttccatgatt tttgccaggc tcgggctggc
    14221 accggggtca ccttgggagg taacggccca gatatgctgg tgacctaagg gcgaagtgac
    14281 ggtttcgtcc catttgcagg cgacgaggta agagccggcg aagaagtccg gatatttgac
    14341 atccatggcg atggatgtca tgccgcccat ggactgcccc gtgttataga tgcggtccgt
    14401 atcgatggaa tattgacggc agagctgttg gacgagatgg atggtccggt ccagttccgg
    14461 cccgtattga tagttgtcat cgacgatgat cgtatcgtat tgaggggcca ggacgaagca
    14521 ggggtgcttg gcctgctatt ccggcgaggc gaaggtaatc caaagcctgc cattttttca
    14581 atttcatgag aaaacctccc ttttagactg tcgcacgaag gcttctgccc tcctgcgagg
    14641 ttctttttct tttatggttt atattatagt atagcatttg tagtttgcag agaaaacctt
    14701 taaatttaaa accatcccct agcccctaac aactaaaaag cgtaaacggg cgccccacgt
    14761 ggtcgcccct acagaaaaaa gggcctgtcg cacgtaatcg tgcacaggct ctttttatgt
    14821 atgcaaaaa
//
LOCUS       DAWCYS010000017        14742 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_272_length_14742_cov_7.780827, whole genome shotgun sequence.
ACCESSION   DAWCYS010000017 DAWCYS010000000
VERSION     DAWCYS010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14742
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_14742_cov_7.780827"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3275 a         3873 c         4189 g         3405 t
ORIGIN      
        1 agttccgttt cgcctttcgg cgtgactttg ccgaccagga cgtcgcccgg gaagccgtcg
       61 gcaccgatca tgatgatgcc gttttcgtcg aggttcttcg tgctttcttc actgacgttg
      121 gggatttcac gggtaatttc ttccgaaccc agtttcgtgt cgcgggcatc acattcgtat
      181 tcttcgatgt ggatggatgt gtagaggtcg ttcttcggga gttcttcact gaggagaacg
      241 gcgtcttcgt agttatagcc ttcccaaggc atgtaggcta cgaggatgtt gtaaccaaga
      301 gctaattcgc cgccgtccgt agccatgccg tcagccaggg gctgaccttc cgtgaccctt
      361 tcgttcttat agaccaacgg gtgctggttg atgcaggtac tctggttgga gcggacgaat
      421 ttgatgagtt tatatgtatc gacagaacca gaatccgtgc ggacctggat ggtgtcgcca
      481 gtaacgcggg atacgacgcc gctgtgtttt gccaggacgc agacgccgga gtcacaagcc
      541 gtcttgtatt ccatgccggt accgacgagc ggagcctgtg tgcgcagcaa aggtactgcc
      601 tggcgctgca tgttcgcacc catgagggca cggttggcat cgtcgttttc caggaagggg
      661 atcatggatg taccgacgga aacgacttcc ttaggcgata cgtccatgaa gtcgacgcgg
      721 tcagcccgga attcacgggt atcttcaccg tgacggcagg caacgcgttc gtggacgaaa
      781 tggccatctt cggtcaacgg ttcgttggcc tgggcgatga cgtactgttc ttcttcatca
      841 gccgtgatgt agtggacatc ttccgtaacg acgaccgttt ctttatcgac gcggcggtac
      901 ggggcttcga tgaagccgaa ttcgttgact ttaccgaagc aggccaggga cgaaatcaag
      961 ccgatgttcg ggccttcagg cgtttctacc gggcacatgc ggccgtagtg ggaatgatgg
     1021 acgtcgcgga cttcgaaccc tgcgcgttca cgggacagac cgccagggcc gagggcgctc
     1081 agacggcgtt tgtgcgtgat ttctgccagc gggtttgtct ggtccatgaa ctgggacagc
     1141 tggctggaac caaagaattc tttgatagca gcgacgacag gacggatgtt gatcaagacc
     1201 tgaggcgtaa tgctttcggc atcctggatg gtcatgcgtt cgcggacgac gcgttccata
     1261 cgggtcaggc cgatgcggaa ctggttctgc aggagttcgc cgacgcagcg caggcggcgg
     1321 ttgcccagat ggtcgatgtc atcgacagtt ccgaagccgt ccatgaggtt gagaagatag
     1381 ctgatgtggg cgatgatgtc ttcgcgggta atcgtgcggt ggtcatacgg aaggtcgctg
     1441 ttgttgcaga tgatcttgta gaccgagccg tccgtttttt cgacgaaggc ttcgacgagg
     1501 ccgccgccgt caccggcggg cgcaccgtcg gcgatttcgc gatggaagac gccgctttcg
     1561 cggatccttt caacttcagc cgagccgatt tccgtatggg catcgacgat gacttcccct
     1621 gtttccgggt tgacgatcgg ttcgtgcaag gtcaggccaa agaggcggcg ttcccagccg
     1681 agtttcttgc cggctttgta acggccaacg cgggccatat cataacggcg cgggtcgaag
     1741 aagaggcctt caaagagatt gcgggcactt tcaacggtcg gcggttcacc ggggcggagt
     1801 ttcttataga tttcaacgag ggcttcgtcc tgattttccg tcgtatcttt ttcaaacgta
     1861 gccttcaggc gtttatcgtc gaagaagagt tcgaggatgt cggcgttcga cgaccagccc
     1921 agcgcgcgga tgaggaccgt gacaggaatc ttgcggttgc ggtcgatacg ggcatagacg
     1981 atgtcgttgg cgtctgtttc caattcgatc caggcaccac ggttcggaat gaccgtcgaa
     2041 ttgtacaggc ggatgcccat cgggtcgatt tcgtggccat agtagacgcc cggagaacgg
     2101 acgagctggc tgacgataac gcgttctgcc ccattgataa tgaaggtacc tgtatcggta
     2161 atcagtggga aatcacccat gaatacttcc tggtccttga tttcacctgt ttcgtggttg
     2221 acaagacgga tctggacgcg gagcggtgcg gcgtatgtcg tatcgcgttc cttacattca
     2281 cgaatgtcgt acttcggttc ccctaattta aaattctcga aggataaaga caggttaccc
     2341 gatgcatctt cgatgggaga aatgtctttg aagatatctt tcagtccctt ttccaggaac
     2401 cattcgtagg atttattctg taaatccaac aaatgaggca ttttcaagac ctcgttaatc
     2461 ttagcatacg tgtatcgagt ccttttccct acttgtacag gtttgaacat gctgattctt
     2521 caccccttat aaaaagtaaa tcattatgat agcgttgtac agcgccgtgt aaagcgacaa
     2581 aaaagcaagg ctctcagacg atttgaacct tgcttaattt tttgctcacc ggaagcgctt
     2641 cctccatgta tctcataata tcaattgaac attttgcaat gagttattat agcatagcaa
     2701 agcacttctg tcaatattct attttaacaa ttttctaaaa tctaccctta ctgatgaacc
     2761 cgggccaaag tatatttctt cttccctttg cgaacgatga cgaacttgcc gttggaattc
     2821 ttgtgaggtt tgacgatttc gtcgagggac gtgactttga cgccgttgat ggtgaccgca
     2881 ccgtgggtga tgtcttcgcg ggcctggcgt ttcgatttgt aaatctggga atcgacgagc
     2941 cagttgacga cgtctttatc ttcgttggaa acgtcgacag tcggcatctt gccgaaggcc
     3001 tgttccaatt cgctttccga caggtcggcg atgctgccgt ggtacagggc ttccgtgatg
     3061 ttctgggctt ccttcagggc ttccgggctg tggacgaaag tcgtgacttc ttcagccagg
     3121 cggcgctggg ccagtcgttt ttccggttct ttttcgaccg attcggccag ggcgtcgatt
     3181 tcttccttgc tgagaaaggt gaagtatttc aagtatttga cgacgtccgc atcatcctgg
     3241 ttgagccaga actgatagaa ttcaaagggt gatgtctttt ccgggtcgag ccagacagcg
     3301 ccgccggcgg tcttgccgaa cttggtgccg tcagatttca gcatgagggg aatggtcagg
     3361 ccataggctt tggcatcgct gccgtgcatc ttgcggatcg tgtcgatacc ggccgtgatg
     3421 ttgccccact ggtcggcgcc gccgatctgc agctggacgt cgttatggga aaagaggtgt
     3481 tcaaagtcga tggactggag aatctggtag gaaaattccg taaaggaaat gcctgcttcc
     3541 agacggctgg caacgacttc cttgctgagc atgatgttga cgctgaacag tttgccataa
     3601 tcgcggagga aatcgaggag gctgatctgg ctgagccagt catagttatt gacgaaggtg
     3661 atgttttccg aatcgccgaa aagatgcatg aactgagcct tcaatttttc ggcattggcc
     3721 ttgattttat cgagagtctg gagctggcgt tccgtcttgc ggccgctcgg gtcaccgatt
     3781 gcaccggtac cgccgccaat gacgacatac ggatgatggc cggcattggc aaagcgttta
     3841 agcatcatga acggaatcaa gtggccgata tgcatgctgt cacccgtcgg gtcgacaccg
     3901 caatagaggg aaatgctctt ttcttccgtg agcttacgca acccttcttc atctgtcatc
     3961 tgattgacgg caccgcgcca agacaattca tcaataatat tcatcgaatt cgctcctttt
     4021 gtataaaaaa tgataagtta ctcttcatta taacgacatg aacgtattac gtcaatgatg
     4081 ggcgccaaag gcgcccgctg ttcgcagacc gcaggccgca ggccgcaaaa ccacgccaca
     4141 ttggcccgct tggtatatgg ctgactattc gcggcgggct cgccacgtgt gagcccccta
     4201 ccgataatta caggaaggct atcggcttac gcggttgacc aggaagccgc cgaggatggc
     4261 cgccgtgagg gacgcggcgg tgatggcggt cttggccgtc atcaggaccg gactgccggg
     4321 aaaggccagg ccggcgatga acagggacat ggtgaagccg ataccggcca ggaggccgac
     4381 ggcccagaag tgtttcatcg tcagcccttc tggcagggac gcttttttca aggccgtcaa
     4441 cagccagacg gccaggccga tgccgatggg cttgcccagg accaggccag ccaggacgcc
     4501 cagtcccggt gccgacgcca ggccggtcag gccgctcagg tcgaggcgga tgccggcgtt
     4561 ggccagggcg aagacgggca tgatgacgaa agccgagaca ggcgtcaggg cgtgttctgc
     4621 ccgcagcagc aagccttccc ttccatcctt gccgggcacg gccggcatgg cgaagcccgt
     4681 gaggacgccg gccatggtcg gatggatacc gccgtagaag aaggccagcc agatgaggat
     4741 acctcccaca gcgtaaaacc aggctgattg ccaattcctg cggcacccag ccagcatcag
     4801 cagccagacg atgaggccgc cggccaggac cggaaggttg agggaaccgg cataaaagac
     4861 agcgatgacg acgataccgc cgagatcatc gacgatggcc agggccgtca aaaagacgac
     4921 gaggctgcgc ggcgctttcg gagccgccag ggacaggacg cccagggaaa aggcgatgtc
     4981 cgtcgccatg gggatgcccc agccaccagc cgtcggcagg ccggcattgc acaggccata
     5041 aatcgcagcc ggcacgagca tgccgcctaa ggccgcagcc atgggcagca tcgctgccga
     5101 ccgggacttc agttctccat agaggaattc ccgcttcact tcggcgccga cgacaaagaa
     5161 gaacagcgcc atgacggcgt cattgaccca gtgcagcagg gacatgtgcg tccctgccgg
     5221 cagcggggcc tggagccagc gttcataagc gggaccgaag ggagaattag agatggccat
     5281 ggcaagtatc gcacaggcaa tgagtaacag tccgcttaac gattctcgtt tcatattttc
     5341 tccttgattg ggtgatgtca cacgaatgtt tctaatatca tgcacggatt ttttctttat
     5401 agcttgtagt atagagtttg tagtttgtag aagatggctt taaattattt acgctttgct
     5461 gcaaactaaa aactgcaaac ttaaaaccgg gcgggccgcc ctttgggacg gcccctacgg
     5521 gttcggacgg cgttcgagga tgacgccctg gcggtaggct tccagcaggg ccttcaggtc
     5581 gtggatcatc tggaggagtt tcttgccgtt atccgtgtcg ccgatcatga cgcgcttcgg
     5641 ttccagttcg acctgtgtcg attcggaatc gatgtcgacg ttgttcatca acgcctggta
     5701 gacatcttca aaggggtagt gagatttgat gcgtatgccg tgggagttga agatgagcgt
     5761 gtagccggca atgcccgtcg acccctggta ggccttgcag aaaccgccgt cgatgcagat
     5821 ttccttgcct tccgccctga tagggctttc gcctttcttg accttgaccg gcgtatggcc
     5881 gttgatgatg tgtccctgag gcgagtcgat gccgaattcg tggaggatct tgatgcattc
     5941 gtctttatcg cgattcaggc ggtagtaggc attctgcggt tctttccacg tcgattcgtc
     6001 tatgatgtaa ctccgttcaa aagtcttgac gatgcggccg gatacagggg aatcggcacc
     6061 gcaccagagg taccacatat agtccaggct gtcagtatcg cgctggtcat aagcccggcg
     6121 gatgaggtgt tccgccttat ccatgaaggc tttgccctta tacggcttac cgtcgaggta
     6181 gatttcctta aagctgccgt cttggttgag gggcaggccg ccgtggaaca ggaggttgtt
     6241 attcatgcag cggtataacg agccgtggct gtagaggaag cggatgtggc gctgcaggcg
     6301 ttcgctttcg gtaaattcgg cctggatgtc gttcatgagc tggtgttctt ccggcgacag
     6361 cgtgtacggg tcctggggat tgaccgtcgg gaagtccttg gtcttcaatt catacgtctt
     6421 gccatcgaga gtgatgacgc ctttttccca atcaatcttg tggaacagca ggcggtcgtc
     6481 catattgtag tcgggatggc ggtaaatggt ctgaccttcc agcttggcca cgatgacgtt
     6541 gatggccttg accatgggct caatgccgtc gtcctcttta tacgtctgca aggcaaagag
     6601 ggcaaagggc cgaaggctga tgccatagcc gctttctaat atttccatat tatggtaacg
     6661 gatgttattg cgcaggacat tggcgatgca ggccgcgttg ccggcagcag cacccatcca
     6721 gaggacgtcg tggttgcccc actggatgtc cagagaatga tgatgcatga gcgtgtccat
     6781 gatcttgtcg gcatgagcgc cgcggtcgta gatatcgccg atgatgtgca ggtggtcgac
     6841 ggccagccgt ttgatgagat tggccaaagc gtaaatgaat tggcgcgtac ttcccgtttc
     6901 caagatggtg tcgagaatct tcatgtgata gaccagctgg ttctggtctt catcgggctg
     6961 ggcgtgcaag agttcatcga taacgaagcg gaagacttcc ggcatggctt tacggacttt
     7021 cgaccgcgta tatttcgacg acaagagctt ggccaggtcg atcatccgtt ccagcgtcgt
     7081 ccgggcccag tcatcgttga ccttgccttc gcggtagagc atgttcagct tttccttagg
     7141 ataataaata agagtcagca agtcgtcctg ttcggccttg ctcatcttgt cagaaaaaat
     7201 catttccact ttttcgcgga tgacgccgga gcagttgttg aggatgtgat agaaggcttc
     7261 gtattcaccg tgcaggtcac tcatgaagtg ttctgtccct ttcggcaggt tgacgatggc
     7321 ctgcagattg atgatttccg tgaacgtcgc cgcctgcgtg ggatactcct tggacaggag
     7381 ctccaaatag cgcaaatatt ccggggaata ggtatcgtac gtttccatgt tgcgtacctc
     7441 tctttcttat atagtatatg ttgtatggct tatttacttt ctattgtata tcatcattgt
     7501 cagggtgact aggttctttt tctgattttc gtcaccttcc tctccatttt gtttaaccgg
     7561 tgtatacaat tattcctcca gcaaaagaca aagggccgtc ttcccccttc ttcctcaata
     7621 agtatagttc tatttatacc atgattttga tttatattgc aattattttt aacgaatacc
     7681 tccctttaac ttactataac gtttatctta taccttaaaa atccttgaat tttgacgtcc
     7741 tctttcacat agtggcaatg cagtgccatc atggaaaaat cttttcctca ttgtggtatt
     7801 tgcaaggacg gaaaaacatg ctattatgta attgtagaaa tcagttttag gtataattaa
     7861 ttttaaatga ggtgattaca tatgaataag tatttggaat ctgtcattga aaatgtacgc
     7921 acgaaacacg caaacgaacc ggaattcgtc cagaccgtcg aagaagtctt cagctccctg
     7981 gaccctgtcg tcgaacgcca cccggaatat gaaaagttcg acctcctcaa ccgtctcgtc
     8041 gaaccggaac ggcaattcac attccgcgtc gtctggcagg atgatgcagg caaataccac
     8101 accaacacgg gctaccgctt ccagttcaac ggcgctatcg ggccctacaa aggcggcctt
     8161 cgtttccaga agaacgtata ctcaggcatc atcaaattcc tgggctttga acagattttc
     8221 aagaacgcac tgaccggcct gcctatcggc ggcgccaaag gcggggctga cttcgacccg
     8281 acgagcaagt ccgacgctga aatcatgcgt ttctgccaga gctacatgca ggctctctat
     8341 cgctacatcg gtcccgatgt cgacgtaccg gctggggaca tgggtgtcgg cggccgtgaa
     8401 atcggctacc tcttcggcga ataccgccgc ctgaaaggct gcttcgaaaa cggtgtcctc
     8461 acgggcaaag gcttcagctt cggcggttcc cgcatccgtc cggaagctac gggctacggc
     8521 gccgtatact tcctggaaaa cgttctcaaa gacgacggcc aggacatcaa aggcaagact
     8581 atcgccgctg ccggcttcgg caacctaacc tggggcatct gcaacaaagc tacggcacgc
     8641 ggtgccaaac tcgttaccat ctccgtcccg gacggctaca tctatgaccc ggacggcgtc
     8701 gctacgcagg aaaagatcga ctacatcgtc gaaatgcgca acagcggccg caaccgcgtc
     8761 caggactatg ccgataaatt cggcgtcgaa ttcttcccgg gcgaaaaacc gtggggccgc
     8821 aaagtcgata tcgtcatgcc gtcggctatg cagaacgacg tccatatgga acaggctaaa
     8881 cagattgctg ccaacaagat ccgctactac atcgaagtcg ccaatatgcc gacgaccaac
     8941 gatgccctga aattcttact cgatcagaaa gacatcatcg tcgctccgtc gaaagccgtc
     9001 aacgccggcg gtgtcgctac atcggccctg gaaatgagcc agaacagcga acgcctggta
     9061 tggactgcca aggaagtcga tgaccgtctc cacgaaatca tgaacaccat ttaccagaac
     9121 tgcaaagacg cagctgcccg caacggcctg ggccacaacc tcgtagccgg tgccaatatc
     9181 gccgggttcg aacgcgtcgc cgacgctatg ctggcacaag gtgtcttcta aggtatacct
     9241 gaaactgaat atatagagcg tgaaaaaagg agctgtcgca tcgcgacagc tccttttttc
     9301 tgtaggggtc gccacgtggg cgacccgttc acgcaaatcc gttttccaag tatttcacat
     9361 atacctaacg ggctcgccct ctacagcggt ccgtttcagc agccgtcttg ccattccttg
     9421 cagtcatgac ctgacaggct gaaaaggcat gtgcgagccc ctacaaacta cctctatcag
     9481 gagaggctct tcgccgtgaa ctcatctttc atctgctggc gcagggccgt gtcctgggcg
     9541 aagagcaggg ccaggcgtgc catgatttcg gcgcctttga cgatggtcgg ttcgatggtc
     9601 ctggccttga cggcattggc gatgtccgtc gagtgttccg gcatggggac gtcgcccaag
     9661 gccaggtgga cgtggacggc cgggcactga tggctgacgt tgccgacgtc ggagctgccg
     9721 caatcctttg gttcttcgta agggatcccc atggcctgga ggacctgttc cgacgctttc
     9781 gtcgccgctt cattccagac catgttatcg aatttgcagc ccatttgggt gacgtcatat
     9841 gtcgtttccg tagcaatggc cgcccctttg aagcagttca tgaccttggc cacgacggta
     9901 tcgagatatt cccgttcgct gtggcggata caacattcga cttcggcgta atcggggacg
     9961 acgttggagg cggccccacc gtttacgata tacgagccga ttcgcgtttc cggccggacg
    10021 tgctggcgca gcatatcgac ggcgtgcagg gccagcatgg ccccattcag ggcattgcgg
    10081 ccgttccagg gttcaccggc ggcgtgagcc gtctggccgt ggaaggcaat gcggtagtcg
    10141 tcgagggcca ggaaatggga attggcattg gtccgggtcg gcgacacgtg gaccatgacg
    10201 gcgaaatcat agtccttgaa gacgccctgg tcgcacatga tgaccttgcc gccgcgcagt
    10261 tcttcgtccg gtgtgccgat gaggtcgacg tcgacaggca gatccttcaa atcctttaag
    10321 gccgcagcgg ccaggaagct catggcacca ttggccgaat ggccgcagcc gtggccgacg
    10381 ccgggcaggg catcatattc cgccaaaagg gccatcttga agacgggttt atcggcccga
    10441 tgagcgatgg ctcggaaagc cgtcggctga ccggtgaact gttcttccac ttccatgccc
    10501 aaagaacggc acaaatcgac gtagtgccgg caggcatgat attcttcacc ggacagctcc
    10561 gggtcgtcag caatggtata gttgagctga aaagcctggg cttcatgggc tttcaaagct
    10621 ttcttcaaat cttcgagggt attcattttc taaaaaaact ccttctatcc aatgataaac
    10681 atatataagg gaatgatgac gatgcccatg acgacggtga taaagctgac ttccgtcgaa
    10741 taacggacat cggcgccgac ggacttggcg acgactgtca tctgggtcat ggccggcata
    10801 ctggcctgca tagtaaagac ctgggccgac atgggcgtga ccgggatgaa gggcaggagc
    10861 accaggacgc aggccggaca gaccaggaag cggccgacga gggcgccgat gatgtcccgg
    10921 tcccagcgga ccgacgccag agaaatgccc gtcatttcaa tgccgatgac catgagcgac
    10981 agcggcgtcg ccatattgcc aacgtattgg aacgacgtca gcaggaaatc aggcagcttg
    11041 atattggcca taatcaggac caggccagcc aaaaagccca ggagcggcgg cgaaaagact
    11101 ttcttcaggg tctgcaggga aaacaagggc atattcccct tgccgcccag actgtcgttg
    11161 acgatatggt agacgccgag ggtccagaac atggtcgtgt tggccatgta atagagcatg
    11221 accgacggca cgctctgcgt cccgaacagg gccaggttga cgggcaggcc gatgaagatg
    11281 gtattggcaa tgaaaaaaca agtgatgaag acgccctgcc ggtcctggcg gatgtgcagg
    11341 actttggccg ctatgcggcc gacggcatag gccaaaagga ttgacgcaaa gggcaggagc
    11401 aggtccggcg cgatgcggag cagcgaatca tgggtaaagt ccttttccag ctggcagagc
    11461 atgtataagg gcaggcttat cttcatgacc agtttggtaa agacagcgct catgtccggc
    11521 ccgaaccagc ctttcttggc caagatgaag ccgatggaaa tgatgaagag gacttcaaaa
    11581 ataccttgta aaccgtgcaa tacgccttcc acgttaccgc ttcccttcta aatgatataa
    11641 ctcaggccgc cggtcgcgaa agacattgat gcggctgcgg atgtctttga tgacggacaa
    11701 gtcgatttcg ccggtaacga tttcctcacc gtctcctcct tgggccagca cttcgcccca
    11761 cgggtcgata atcatagaat ggccacagaa gcggtagtca ccgcaggtac cgcagctgtt
    11821 gacggcgacg acgaagaact ggttctcaat agcccgggcc cgattgagta tctgccaatg
    11881 agccagtctg ggatgaggcc aggcggccgg gacaaagagg atctgggcac cggccagggc
    11941 cgccatgcgg acccattcgc caaagcgcag gtcataacag gtaatcgacg ccatagggat
    12001 gtcgcccagg aagaaagtat tcaagtgatc tcccggcgcg aagaccttct cttccccacc
    12061 aggcgtgaat aaatggcatt tgtcatacga tgagacgcga ttcccctctt catcgacgat
    12121 atacgtcgta ttgtacaggc acccgtcgtg acggcggaca atggagccgc cgatgagctg
    12181 gatatgacag gacgcagcca ggtcctgcaa aaacgcctgg gcctggcggc cctcttcatc
    12241 ggccaggtca taaacatcag gcgggtagaa cgacaggtcc cacaattcag gcagcacggc
    12301 catatccgaa ccagccctgg ccgcctgttg gacgcggcgc cggacctggt cgaaattggc
    12361 cagcctgtcg ccggggacga taggaaattg aatgagtgag attttcactg cacttccccc
    12421 tttctttaat tttcatgtca atttagcttt attatatacc atactatagt atgtaaaaag
    12481 tattttttag aataccgctg gacgacttga aaagaggcgg ccgccttgtt atagtaaaat
    12541 taattatcgc ataaagaagg ggtatcatgg aagaaaatga aaagatgaaa gattggctga
    12601 cagaactcat gagctgtaca gcctgtcact tacgggacga aggcaatcgc ggaccgacgc
    12661 gctattccgg cgattgtgcg tcgccgctga tgttcgtcgg cgaagggcct ggcggtgtgg
    12721 aagatgaata cggcgttccc ctcgtcggcc cgtcggggca gctcctcgac aaggccttgt
    12781 ggagcgtcgg actcacgcgg gaccacgtct atgtcaccaa catcgtcaag tgccggccca
    12841 agaacaaccg cacgccgacc ctggaagaag ggcgccactg cgcggacatc catttggtca
    12901 aagaaatcga actggtccag ccgaaagtca tcgtctgcct aggcaaagtc gccttccagt
    12961 atttctatgg ccgcgccgcc agcatcatgc gcaaccgagg caagtggttc acgtggcgcg
    13021 gctatgacat catgccgacg tatcacccgg cctttctcct gcgccagacc ggccacgaac
    13081 tggtcgaatc gaaatggcag gtctactacg acttcaaagc ggctgtcgaa aaagccaaag
    13141 ccgccatgac cgactatacc tatcagagcc cggaaaagcc ggacctcctg gcccaatatg
    13201 cagcattaaa agagagccgg cattggtaaa tggcccttga aggctgcagt tcgcaggccg
    13261 tgtaaaaaaa ggctgtcgca gatgcgacag cccttttttc tgtaggggcg ccacgtgggg
    13321 cgccccctag aaactctgac gaaaaaggtc actacatgat gaccataccc atcatcagtc
    13381 aaaaatacgg tcgtacaggt ggctccgttc gaggaattct tcgactgtcg ggaagccgag
    13441 tttcttcaat aattcgatga cgtagggatt ttccatgggt gtcttgtagg ccgcctgttt
    13501 gccgtaggcc tggacgttga ccttgccttc ttctttcttg atgatggccc ggggaccgcc
    13561 gacgcatccg cccagacagc ccatgccttc aaagaagttg ccatcgcggt tgccggccag
    13621 gatgtcgttg atcatggcct tgcaggccgg gacgccatcg gctttgcgcg tcttgatgtc
    13681 gactttgcgg tccgggttca gctgatggac cgtttccgtg acggcttcag agacgccgcc
    13741 ggcataggcg tagcagatgc ccgcttccga agcgtggggc acgttgtctt ccggcaggga
    13801 tgccaggtcg atgtccgtaa ccgtcaggaa gtcgcgcagt tcctgatagg tcaggacgta
    13861 atcaatagcg cctaccaggt ccggttcttt ccgttcagct ttcttagcca ggcagggccc
    13921 gatgaagaca gtcttggcat taggatgcag ggccttaact gtacggccgc cggcaatcat
    13981 aggcgagaca gagcccggga cgttgggcag gagctggtga tagagcttct tgatcatggc
    14041 aatccacatg gggcagcagc agctggtgag ctggaactgg tcttcattgt tgatgttctt
    14101 gtcgaattcc agggcttctt tcaatgtcag gatatcggca aagacggcga cttccagcat
    14161 acccgtgaaa cccaggcgct tgaaagctgt ccgcagtttc cccatggtaa catcgggacc
    14221 gaactgaccg gatacggcag gagcaaccaa ggcgtagata gggccgtcgt agtcgtgcag
    14281 ttcctggacg acagggataa ggtctttccg cgtcttcagg gtatcgagct tgcaggcatc
    14341 gacgcaggcc tggcagccga cgcactggct gtcgtcgatg gagatgccat cgggaccgga
    14401 cgtgatagcg ccccagtcac aggcttcctg gcagtgatgt ggttcatcgg gcggacagag
    14461 acagggcttg acccgccaga tcagggaatg gtcgtcggga tgagccagac aatcgatcaa
    14521 tttcgggtca ccatcgacac cgaccggttt accgtcagca gcccgtttaa gcatctgctg
    14581 gtacaattca tctaatgttt tcataaggaa cctcctttgt gaacatctgc taacacagac
    14641 taatacagct ttattgtagc agatttttcg ttattacaac agttttattg tgaacgtact
    14701 cccgcctata gaggcgggag cttcctgctt caacgagaac ag
//
LOCUS       DAWCYS010000018        14681 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_274_length_14681_cov_9.225010, whole genome shotgun sequence.
ACCESSION   DAWCYS010000018 DAWCYS010000000
VERSION     DAWCYS010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_14681_cov_9.225010"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3210 a         4415 c         3695 g         3361 t
ORIGIN      
        1 aagtttgtag tttttaggga attgttttaa atttaaatcc atcttctaca aaatacaaac
       61 tccgcactac aaactaaaat taaaagaacc actcatgccg gccaacgcct tcacgcggca
      121 aaaaatcaat ggaaaatggc gcaggccgtc ttgaggaagg gcaggcgttt gctcaggccc
      181 gtccgcgacc gctggatcca tttgaggtca tacggttcaa agacgacatt gcggtaggct
      241 tcgatgtcca agggcgtccc gtcccaatcg gaatggatgg ccccggtctg cttgatgaga
      301 atatcataga ggatatcgta ttccttatcg tcccgttcat cccgttcgat gaccgtactg
      361 tacggcaggg tcttatgata ggtcagttcc atgcctttgt aatcgaaatg gaaaccgcag
      421 cgcatacggc agccgtcgag gcccgtataa tcggcgattt tcttcaggtc atgaaggata
      481 tggtcatggc cttcttcata taaattatcc ggcgtcgttt ccgtatagtc gaaacgaaac
      541 tggattgatg ccccatcaat cttgcggaac cgttccagat acggtttgag ctggtctgcc
      601 ggatatttct tgtacaatac gcagttgatg cggaacggaa cagccagacg gctcaggagg
      661 ccatcattcg attcctgtac atagtgctgc atatggcgcg atacgttgat gcaggtgatc
      721 ttatgacggt tgcgttccgt aaaagcgacg atgtcgtctt ctgactgatt ctcaaagacg
      781 ggcagcgtcg tattgatata taccttgtgt gtcacaggaa tacagtccag catgcgctgc
      841 agcgattcca tattggcaaa aggttcaccg ccagtgaata cgaaatcaca gaacggcgtc
      901 aagctgtcca tcaggcggat gctctggcag atggcttctt cagaaaaacc cgtcatatct
      961 tcatattccg ccttgttgat gcagaacggg caatggttgt gacaattata gggcacgaaa
     1021 accgtgacgg tagcgccgcc gttccgtgtc ttatacgggt ggaccacacg cggtacagtc
     1081 gttgcggcca tgctgattgt tgttttcatt cttttttcgc ctcgaatcgt ggataagatc
     1141 ccaagctgtt cccacacagc agtaagatgg aaaggaaagt attttttcac ctcacagcag
     1201 acaatcttgt gacccgaatg tagtatccac gcttattgta ccataaaagt ccatttctgc
     1261 aaatgcatgt tgcgtattcc cattttgcat aatttttata ccaaacaatt catcataaac
     1321 attattttat ttttctaaag aaatccttac atttcctttg aatctaaagc ccggctaatg
     1381 tcagccgccg tctttttgac agcttcgatg gccaggtccc gtccttccgc cgttagccgg
     1441 tctgccggct cggatacact gatagccgcc gtaatgattc catgatggaa aatcggcgcg
     1501 gcaatgcatg ccatgccgag ttcgacttct tcccgttcca cggcataacc ccggcagcgg
     1561 atcttctcca gttcctggag caggtcgctc cagtcggtca cggtatattc cgtaaacttc
     1621 tgcagcgtgc agcccgtata agtgtgtagg tattccggtg aaaaagccaa gaggcacttg
     1681 cccgaggccg agcaatacgc cggacggacg gcaccgatag gcggtgccgt atcaatcgtc
     1741 ctgggcgact tgatcttctc taaaatgatg tgctgcggat aggaagcccc attatgggca
     1801 aaggtcgtaa tatggacccc ttcattgcac tgcagggaca gggccttggc atacggatag
     1861 gcgactttag ggaaggcatc cttttcccgg aagagcatcc ccagggcata gacctggagc
     1921 cccagccagt atttccctgt ttcactgtct ttctggacaa agccgcggcg ctccagcgta
     1981 tccatagtgc ggctgacagt actcttatga aggtccatca gcttgctgat acgggtcaca
     2041 ctgaggggcc tttcttcctt taaaaaaagc tgtaaaacgg ccagggcccg gtcaatcgat
     2101 tcaatcatcc tcgttccccc gatccggcat cgtcacgacg ccaagaaatt aatatacttt
     2161 attatatgga atacaggcat aatgtcaaga gggaaacgcc aggccccagc cggagagtgc
     2221 tagtggctga ccgcttcgct ttcttcctgg gacgtctgat gatggtcgta caggcactgc
     2281 gacagcttgc ggatcaaatc gtcacattcc aaattgccgc gccagctctt ggggatagcc
     2341 tggaccccat cctgggtacc catgatattg ccgcagatgg cgccgcacgt atcgctatcg
     2401 ccatcctggt tgcaggccat gagaactgcg tctttcagat ggcccgtctt gaggacgcag
     2461 tatacggcca gggccagggc ttcatcggct ttcttgccca ggcccagctt cttcatggag
     2521 cgcactggat tgcggtcggt gacggcttcg tcgacagccc gcaggatagt cttcatgagc
     2581 ggcgtccctt ccggatcagc ctggaggatg cgcagggccc ctcccagggc agcgctgata
     2641 tccttaccag ctgccaggga ggcaatcatg gcactcagcg tggccgtcga taaataggct
     2701 tccggactac cgtaggtcag gctggcagcc tggcagccga cggcaaaggc tttttccggg
     2761 tcgccggcat aaaacaggcc gataggcgca gcccgcatga ccgtactgct gccacaactg
     2821 tggttcagcg gttcctgacg ggtcaatttt ttccccgacg ccagcgacgt caggcaggtc
     2881 ttgcccggag agcggcgggc aaagagggcc tcttccccca tgatgtcgta gtcgaaatcg
     2941 acttcatgag gctggcgctt catccatttt tcctgttccg gatggatgat gcgttcggtc
     3001 tgcgtataat accagcgcat atacgcccgg tacaggccat ctgaaggttc caatccgtcg
     3061 tggtcggccc agagcatgcc gtcagctgtg aacaacgcca gctgcgtatc gtctgagata
     3121 aggctcttat tgccattttt agccgatttt aaaacagtgc gcaatccata agggccgtat
     3181 ttcttatgaa tcgtcttcaa atcgtcgaat tcaatgacaa accccaaagc gtcgcctacg
     3241 gcgccgccaa acatgcaacc cataaaccga tctagtccat ccatgtagca tcctcctatc
     3301 ctcgtctgtc gtccgagtct ttttttctca aaaaacttta caatttcgtt caagtagtat
     3361 ataataaaaa gcttatacgt tcattataca ggttatgata ggaatttgca aataaaaaaa
     3421 gggcttgtcg cacgaagatt tttgccatca tgcaaagccc cttttacttt atagtttgta
     3481 gtacggagtt tgtagtttgt agaagctgac tttaattttt ttaccatttg ctgcaaacta
     3541 aaaactacaa acttaaagct caaaaagagg ctgtcttaat gcgacagcct cttttaagtt
     3601 gttagtggtt agcagttagc cacgagccac gagtcacgag tcacgagcca ccaacatttt
     3661 aactcatttc cccgctgcga ggaggtcggt tctgccttct acgcggtcga gggctttctt
     3721 tacgatggcg gccaggccct gcatcttgat ttccgggatg gagcagacgc cgaggcggac
     3781 gccctgggcc aggggaccgg caaagaccag gtcggcttcc aaggcctgac agacggcagc
     3841 cgattgctgc gtcgaaatgg agatgaagaa gccgccttta tagggcacat acggcaggcc
     3901 gcagcggtcg gcttcatagg tgaagatgtc ggcacggcgg cgcaggctct tggcaaaact
     3961 catgcgttcc aattcgagct gggccatgag gtcgcggtcg ctgcggatct tggtcaggag
     4021 cgtcatggcc gcgcggttga tgttcgacca ggccgtgcgg gccgaatatt tgcccagatt
     4081 ggcgaactct tcgataacgg ctttgctcga cgacaagccg acgagagcgc cggcgcgctg
     4141 gccatacata gtatagcctt tggacatgct gaaggcgaac atggtaaaga tgtgttccgg
     4201 aaggccggcg aattgtctca tgaacgagcg gacgcggtcc ttttccccgg cataggcgat
     4261 ataggcgata tcgacgagga tactgatgcg cttgccattc ttttcatgga ggcggcagac
     4321 ttcgaggaca tcggcccatt cggcatcgga cagggcaaag cccgtcgggt tgttggccgg
     4381 cgtgttgagg atgaggagca gcgaatcctg acggcgcagg atgttggaaa cagcagccga
     4441 gaacgccgcc gtattgaaat ggccctggtc gtccatcatc gagaaggtgt gcaggtcgcg
     4501 gccgatttcc tggcacaggc agtcataaat gttccagcgc cagtccgtcg tcagtacggc
     4561 atcgccgact tctgaatagt tggcaatggc gatatggatg gcaccggtac caccggacgt
     4621 agccacagca tcgacatagc catcgggacg acagccttca aaggtttcgt cgatgacggc
     4681 ctggcgatag ccttccaggc ccagcggcgg tgcataggta atgaaatcgc tcataggcag
     4741 agagcggtat aattgttcta ccacaggcag gcaagccagt ttttcatcgt cgccggcata
     4801 acagccgacg gtcgcattga cgaccttatc cgccccgtac tgcgctgccg cttcccgggc
     4861 tgccgctcca gcggcgaata ccacgtcacg taatgtctta ccttctctgc ttgctgctgc
     4921 cataatcgtc atcgtaaaaa acccctctct gcataatagt tcgtgtttag tggacacttg
     4981 ttctctccaa atgtcctgac gtcctacatt atagcactgt gaaaagagtt tttatatagc
     5041 gaaaagccta aaatactgta tacacagcat gaatattgta tacaatattc atttgtggtc
     5101 ttcgctgtcg tgtctggcac cggcatcgac gtggaacgtt gcctgtttga tgcccttcat
     5161 ggccaggagc tggctgacga tttccgtttc atccatgtaa tgagccgccg tcagttccag
     5221 gtcgatgtcg gccaagggtg catcgtcatc attatgggaa tggaaggtct tgatatgcat
     5281 gtggtggtca tggataaaag tcgaaagttt ccgggcctgg ccgatgttgt tcttcatgac
     5341 caggtgcatg gcatagcgct tgcggaagtg cccttccagg cgggcatcga ggcggacgaa
     5401 gacggtcagg accaggaaaa tcagcgccgt cgccacacag gctatgacga gatagccggc
     5461 accgacgacg aggccgatgg ccgatacggc ccataagccg gcagccgtcg tcaggccgcg
     5521 gacgctgcgg tctttctggt tggccaggat cgtaccggcc ccgatgaagc cgacgccgga
     5581 tacgacctga gctgcaatcc gttccgggtc ggagttatta tagccaaagg tgaagtaata
     5641 atccatggca atattgatag atatgatcat gcacaggcag gagccgacac agaccagggt
     5701 atgtgtcctg aggcctgccg acttgctctg ggcctgtcgt tcataaccga tgagtccgcc
     5761 caggatgagg gccataatca ggcggatgat gctgtcttcc gtagaaatca tagaggctca
     5821 acttctttca aaataatttt tacatccgtt aagaaaacgt tataatggta ttataatata
     5881 tagatgcagc agagaacaag caaaatgttc tccccgcatc ggagaaagaa ggtcatgatc
     5941 atgaaccgct tcatttgctt tgataatctg cgcagtgccg ccgtcgtatt gacgctggcc
     6001 gctttattgc tacaatcctg gacagccttt tacgaagaga gtgccgtcgt cgccatttcc
     6061 atcgtcttct tcatcagtgc ctacttcgga gcttcggccc tgcgcatccg cacgatatcg
     6121 ttcttcttga aagataaatg gaaacgtctg ggctggccgg gcctcgtctt agtcctcttc
     6181 ggcatcgaag aacagctggt cacatcagcc ctgccggtca cgtcgtgggt cccgctctgg
     6241 ctcttcctgc tctttgccgg cttgtccctg atcaagcatg tcaaccataa ggtcttgcag
     6301 caccgcatcg cctcgaagcc atcagttcct ttcctgctgg gcttcctcgt cttgctggca
     6361 gccgccctct tcctctgtca ggccttcctg cctcagtccc tggccatccc ttacatcgcc
     6421 cggatccgcc cggactggct ggtcctgtcc ctctttgcct ttgccctagg ggtctacacc
     6481 ttccgccatc gctggttcac gccgtctggc tatcagccta aaagcagcag cctcttcggc
     6541 ttcgccatca tggtcggcgt ctatgccgcc tggatgcccg gcaagggagc ctctatcctg
     6601 acagcgctga cgccggcttt actgctcgtc gccgccgtcc ttggcctgac ggcctggttc
     6661 catcagcagt tcgataagca ctcggcctgg tcccacacgc tgcaccgcat ttcctacggc
     6721 ctgttcttcg tcagcgaacc gatcatcctc cattcgatgt ggttcttaca gcccctggcc
     6781 gtcgccgtat ggctcaaggt cgtcattacg gccctggtcc tggccatcta cggctatctg
     6841 ctctgccgtt acgccctgtt ccacatccca tgctttaaaa gcaagggata atagctggcg
     6901 gacactagcc ccggcaggcc atctgtgcta tgatagatag caagtttgtt tctttacatt
     6961 atcgataagg agtgtgccta cttggataca ttattacaac tcatggaatc gtacggctac
     7021 atcgcgatgt tcgtggcaat ggcattggaa aacgccaata tccccatccc cagtgaagtc
     7081 gtactcggat ttgcaggctt cctcatttcc cagcaaatct tttcattctg gacgaccttt
     7141 gccgtagcct gcgtcgccgg cctcgtcggc tcggtcatca gttactggct cggctcttat
     7201 ggcggccggc cgctcttgtt aaagtatggc aaatacatct tcttcaacga acgtaaattc
     7261 cgcatggccg aagacttgtt caacaagtac ggcggcattg ccgtcatcat ctgccgctgc
     7321 ctgcccggcg tccgcacgtt tatttccttc ccggccggcg tcgcccgcta tcctttctgg
     7381 aaattcgcca tcttcaccgt catcggcacc atcccctgga cgctgctcct cgtatgggcc
     7441 ggctccctcc tgggcagtca ctggcgggac ctcatacagt acaaccacat cttcctcatg
     7501 gccgtcatcg ccgtctgtgt cgtcgccacc gtcgtattcg tatggcatag acgccgccgg
     7561 agaaatgcat catgaaacaa tggttcttaa cacatcgtta cggcctgacc gtcgccttag
     7621 tggcagcaat gatcttgctg ccatttttag gtgctgcccc gctcatggac ccggacgaac
     7681 cggtctacgg cgaaacggcg cgggaaatgc tcctggctgg ggattggctg tcaccgcgca
     7741 tcttcggcca gtactggtac gataaaccac cgctcttcta ctggctggaa atgctgtcgt
     7801 acagcttttt cggcgtctct gacatcgcat cccgcctgcc cagcgtcatc atggccatgg
     7861 caacgacgat gtacgtctac gtccagggga aggaattatt caataaaccc atcgccttcc
     7921 tgtcggccct gttcctggtg accagtgccg gcttcatgta tatcggcaag gctgccgtca
     7981 cggacatgac cctcatgttc accctgaccg tgaccatggt ttccttttac cgtaaaaact
     8041 attatatagc ctatatcttc tgcggcctgt cgctcctggc taaaggccct gtcggctacg
     8101 cgttcccggc gctcatcatg ctcatttatc tggcctggac gcggcagtgg ccgctcttga
     8161 agcatatgaa gattccccag ggcatcctgc tggccttcct ggtcggtctc ccctggtata
     8221 tcctcatgta ccgggtccac ggcgaagtct tcctggacac cttcatcggc tatcacaaca
     8281 tcacccgctt cgcggcgccg gaacatccgg gacagaacag tctcttcttc ttcatcccca
     8341 tcctcttcgc cgccatgatg ccctggacgg cggccctgtt cccgacaatc cggcggctgt
     8401 tccacaagcg ggaccccttc cgcgacatac tccggttctg cttcgtctgg gcggccttta
     8461 ccttcgtctt cttctccctc tccaagaccc agctggtcac gtatatcgcc ccgatgttcc
     8521 cgccgacggc atatctgttg ggctggtact ggtaccgctg ctatatcgag aggaaatttt
     8581 cgcggccctt gctggtatcc ttctatatcc tgggcctgct gctgctcgcc agcaatgcca
     8641 tcccgctcca cgaaaacgcc gcctttttcc gaccggccat cgcctggggt tcgggcctgc
     8701 tggcagcggc attattcctc ccggcctggt gtctgtggcg ccgccgctac cggtcggctt
     8761 tcgtggcagg catcgtgatg atgatctgtt tctcctgggc ggctttcggc ttcatcatcc
     8821 cccagttcaa aggccacgtc acatcttacg agacagccca aatcctgccg gatgtctatg
     8881 acggccattc cccactctat atcgaaaaat tcctgcgccc gggcatcgcc tattacagcg
     8941 gcatgtacgg aacggaatgg ggccccgtca aggacccgga cctggaagaa ttgctcgcag
     9001 agaaggataa gattttcctg gtcatgtcga aatcgacctt ccataagctg gccaaatccc
     9061 agcaattgat tgaccgcttc ggcatcgccg ccgaaacgcc gacacaagtc atcctcatca
     9121 accatcctta ggaggtgtcc ccatgaaaca gcgcacattc ctgaccatca tcttcgtggc
     9181 agcattcctt ttcctggccg tcggcatatc tcagctggcc gtcacggacc cggtcgaatc
     9241 gaattacgcc ctgacggcac tggaaatggt ccgcagcggc gactggctgt ccccgcagat
     9301 ttacggcacg tactggtatg ataagcccat tttcctgtac tggctcctga gcctgtctta
     9361 cagcctcctg gggacgacgg atctggcagc ccgcctgcca gctgtcctct tcggcaccgc
     9421 ttcctgcacc ctggctgcct ggttcgccct gcgccagacg ggccgccgca cgacagccat
     9481 cctcttcgcc gccatgaccg tgacatccct gattttctgg ttcgtcagcc gctccgtcat
     9541 cactgaccag cccttgtatt tcttcagtgg cgctaccttg tttttcgcct atatcggcct
     9601 gacagaaggg aagaaaggct atatgacagg ggcttacgtc atggctgccg gtgccgtcct
     9661 gaccaagggc ccggtcggcc tggtcctgcc cggcctgttc ctgcttctct ttaccgccat
     9721 ccagcgccgg cccgattacc ttaaaaggct gttcccgccg gcaggcatcg tgctcttcct
     9781 gctcctctgc ctgatctggt atggccccat gtacagccgc catggcatgg atttcatcga
     9841 tggcctgctg ggcttcaaca acgtcgtccg ggcgaccgta tcggaacatc ccgaatacga
     9901 cgtctggtat tactacctcg tcctcgtccc aatcagtctc ctgccctgga ccggcccctg
     9961 cctctacgcc ctgtggcggc ggcgcagtca tcacgatgag tacgtattca tggccatctg
    10021 ggccctcggg acaatcctgt tctacagcct catggcaacg aaatacccaa cctatgccta
    10081 tatcgccaac tggcctctct tatacctggg cgcccgcacg atgcaggcct ggctcgacga
    10141 ggacgcccgc tatgcctggc tggcagccct catcccggcc tccgtctaca gcctgctctt
    10201 cgccgtattg gtggcctttg cggataaagc gcctttcccc gtccaaggcc tgttgctgct
    10261 gacggtattc ctggccatca tggctatcct gacctggctg gcgtggtggc agaaagccta
    10321 tctggccctg cccttcttcc tgattacgac gacagtcatc acctacctga tcgtgacctt
    10381 ccaggtcctg gtcccctttt atcaataccg gtccgcccaa tccctgaccg tcctgaacga
    10441 tgcggccccg gtctatttct ttgaagagta ccgcacgtcc tatccttatt atacgggaca
    10501 gacggcatac tggacggcac cggccgacta cgacgaaaat gcccggctcc ggcgcgatgc
    10561 cgtatgggct aagaaacacg tctacccgac agcttccgaa gaccaggtgt tatccagcct
    10621 gaatcagcac cagaacctga ccctcgtcgt cccggaaggg aaatataaag attataaagc
    10681 ctcggaattt ttcagcctga cccggaaagt aggccggatc ggcccgtatt atatcttcaa
    10741 gcccagccct taggatagga ggattttcca tggaagaact catcgacaaa ctctgtgcct
    10801 accatcatcc cggcgtattc aatccctggt ccgaatatga cgtctcctgc gacatcagtc
    10861 cggacgcgcc agccatccgc tgccagcagc tgcgggaata tttggcgccc cgcctgaccc
    10921 gggcccgcat cgtcctcatc gccgaagccg ccggatacca gggctgccgc ttcacaggca
    10981 tccccattac ctgcgaacgc atgctcctgg acgaacacaa acagatacat gctgacgtca
    11041 tcctgggcca ccgcggccag cgcacgagcc ggcccgacag cgccttcatg accagccgtc
    11101 ctcagcggga aaaaggcatg aacgagccga cagataccta cgtctgggga gccatcgtcg
    11161 acaacggcct ggacccgcgc gacgtcttat tatggaatat cttccccttc catccccaca
    11221 agacctcgcc cttctccaac cgcacgccga cggacagtga gctggccgac gccctcgtgt
    11281 acgccaaagc cctgctgagc tactgccggc cggatatacg cctggccgcc atcggcagga
    11341 agtcagcaga aaccttgcag aatgccggct tcccggccat cgccatgcgc cacccggcca
    11401 acggcggagc cggtcttttt cgggaacagt tcacacgttt ttctcgataa ttcctttaaa
    11461 aaatcggtaa aattcgattt ttacattaaa attgtcggcg tttcagcaaa aacattcaga
    11521 aaaatcattg catcgacttc acaaattgtt tataatcata ggtacaccac agtatatacg
    11581 agccttctct ctcatacagt gacggctcgt ttttgtatac ccaaaggggg attacgtatg
    11641 aataaagaaa tgcgcgatct gccgcgcccg caaggcctgt acgatccgtc ttatgaacac
    11701 gatgcctgcg gcatcggtat cgttgccaat ataaagggga aaaaatcata cggcatcatc
    11761 gacgatgccc tgacgatcct ggaaaacctc cgtcaccgcg gtgccgaagg ggctgacgcc
    11821 aacagtggcg acggcgccgg catcctggtc cagattccac accagttctt caagcgcgaa
    11881 tgtgaagtcc tgggcttttc ccttcctgac gaaggtgaat acggcgtcgg catgattttt
    11941 gcccatcgtt atgaaacatt ccgtcataaa cagatggaag cctttgaaga catcgtccgc
    12001 tccgaaggcc tgtccatcct cggctggcgc gatgtccctg tcgatgaaag cctgatcggc
    12061 aacattgcca aacagacgtg tcctcatttc ctgcaggtct tcatccgcaa ggacccgacc
    12121 atgaagaccc agatggactt tgaacggaaa ttatacatca tccgcaagct gacagaaaaa
    12181 gccatcgtcc cggaaagcca gaaaaacggc acggacttct acatcgccag cctgtcatcg
    12241 aagaccatcg tctataaagg catgctgacg tccatgcagc tgcgccactt cttcctcgac
    12301 ctgtcggacc tggatttcgt cacgtccatg gccctcatcc attcccgttt cagtaccaat
    12361 accttcccca gctgggcccg ggcccatccg aaccgttaca tcgtccacaa cggggaaatc
    12421 aacaccatcc agggcaacat caactggctc aacgcccgtg aaggcaaatc gaaatcggac
    12481 ttcttcccgg acatggaaaa agtcttcccc gtcgtcgacg atacgggcag cgactcgtcc
    12541 atgttcgaca actgcctgga atacctgtac atgacaggcc attccctgcc ccatgccatg
    12601 atgatgatga ttccggaacc gtgggaacgg gatccgctca tgagcgacga aaagcgcgat
    12661 ttctaccgct atcacaactt catgctcgaa ccgtgggacg gcccggctgc catcggcttc
    12721 tgcgacggca cggtcatcgg cggcatgctc gaccgcaacg gcctgcgtcc ggcccgttac
    12781 tatgtcaccc gcgacgaccg ggtcatcgta agttctgaag tcggcgtcgt caacatcccg
    12841 ccggaagacg tcctctacaa gggccgcctg gaaccgggca agatgctcct cgtcgatacg
    12901 cagaaacagc gcatcatcga cgacgacgaa atcaagcacg aaatcgcgac ggaacaccct
    12961 tacgacgaat ggtataaaga acacatcgtc aacctcgacg acctcatgac gggtcagaac
    13021 atcgccaaca gcgatgccat catcccttat gacctgaagg aacaggaaaa agtcttcggc
    13081 tatacccagg aagatatgga caaggtcatc ctgcccatgg cccgcgatgc ccagcacgcc
    13141 atcgactcca tgggcgtcga cgtccccctg gccgtcctca acgacaaacc tcagctcctc
    13201 tacgattact tcaaggaaaa tttcgcccaa gtcaccaacc cggctatcga cggtgtccga
    13261 gaaaagacgg tcatgtcgtc agctgtcatg gccggcaacg tcgccaacat catggaaccg
    13321 aacgaaacgt cgacggcagc cttgtacctc aaaggcccga ttctgaccaa cgaacagatg
    13381 tcggtcatca agtccctcat gacgccgaaa ctgcgggcgg ctacgctgtc catgctctat
    13441 cccgtcaaca gcggctctga aggcatggaa acggccatcg aatcgctctg catcgatgcc
    13501 ttgaaggcca tcaagaacgg cgccaacatc ctcgtcctgt ccgaccgcgg cgtcaactcg
    13561 cgcatggccg ccattccggc cctcctggcc tcggcagccc tgcaccatta tctcatcgac
    13621 aagggcgtcc gctccgacgt cggcctcatc ctggaatcgg gcgaaccgcg cgaagtccat
    13681 catttctgta ccctcatcgg ctacggcatt acggccatca acccgtacat cgccctggaa
    13741 tccatcaagg aactcattgc caacaagaag ctccagaaaa tcgattacga aacggccaaa
    13801 cagaactaca tcgacgccgc cgtcaaaggc atcctggccg tcatgtccaa gatgggcatt
    13861 tcgacagtcc acagctacca cggcgcacag atttttgaag ccgtcggcat caaacaggac
    13921 ctgatcgaca aatatttctg caacacgccg tcccgcatcg aaggcctggg catcgctgaa
    13981 atcgccaagg aaaacgtcct gcgccaccaa gaagcgtaca gcacgggaga taaactggaa
    14041 cggggcgact tctaccagta ccacaagggc ggccagccgc acatcatcga cccggaaacg
    14101 gtccagctcc tgcagaaagc cgtccagacc ggcgactacg caacgtataa aaaatacgcc
    14161 gaccatgtca accgtcagac cattttccgc ctgcgcgacc tcctggactt tgaatatcct
    14221 gccggcagca gcatccctat cgaagaagtc gaacctgtcg attccatcgt caagcgcttc
    14281 cgcacgggtg ccatgagtta cggcgccctc agcaaggaag cccatgaatg tgtcgctacg
    14341 gccatgaacc gcctgggcgg catgagcaat accggcgaag gcggcgaaga ctcggcccgt
    14401 ttcgatacgg aaacgaacga ccgcatcaaa caggtcgcat cggcccgttt cggcgtcacc
    14461 agcgactacc tcatccacgc cgatgaattg cagatcaagt gctcccaagg ggctaaaccg
    14521 ggcgaaggcg gccatctgcc gggcaataaa gtctatccgg atatcgccaa gacccgtcat
    14581 gctacgccag gcgtcgccct catctcgcat ccgccgcatc acgatattta ctccatcgaa
    14641 gacctggcgg aactcctctg tgccctccag caggcgctcc g
//
LOCUS       DAWCYS010000019        14460 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_283_length_14460_cov_7.429434, whole genome shotgun sequence.
ACCESSION   DAWCYS010000019 DAWCYS010000000
VERSION     DAWCYS010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14460)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14460)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14460
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_283_length_14460_cov_7.429434"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3109 a         3777 c         4179 g         3395 t
ORIGIN      
        1 ggcatgaccc tgccgacgaa aatatctttg ttcctcggca aggtgggcgg catttccgtt
       61 ctctccgtcc tcctcggcgt catcatgttc ggcatgggca tgaccatgag cgtccacgat
      121 ttcgccctgg tcctcaagcg gccgaaagat gtcttttttg gcgcctgcgc ccagtatttc
      181 gtcatgcctt tcctggccta cgtcctttcg acgattttcc agctcgaccc ggccctgacg
      241 gttggcgtcg tcctcgtcgg gacctgcccg ggcggtacat cgtctaatgt catcaccttc
      301 atgagcaaag gcgacgtacc cctgtcggtc acgatgacca gtgtatcgac gttaatttcg
      361 ccaatcatga cgccgctttt gacgtatctc atcatcggtc agcgcatctc ctttgacccg
      421 gtcggcatgt tcttatcgat cctgcagatc gtcatcgtcc ccatcgctct gggcctcatc
      481 gtcaagagct tcctgccgaa gctggctgat acggctgcgg attacctgcc ggctgtttcg
      541 gccctggcta tttccttcct catcgccggc atcatcggag ccagccgcga tgctatcctc
      601 aacagttcgg gcatcatcct gctggtcgtc atcctgcaca actgcctggg ctatgccctg
      661 ggattcttta tcggccatct ctgcggcatg agctggaaga aaagggtcgc cttgtccatc
      721 gaagtcggca tgcagaattc cggcttagca acgggcctgg ccaaagcaca ctttgcatcc
      781 ctgcccatgg ccggcgtccc cggtgccgtc ttcagtgcat ggcacaacat ctccggtgcc
      841 gtcttggcct atctctacgt caactacctg aacaaacgct tcgactccaa ctacgaaacc
      901 gaccagaaag ccgctgccag cgtatcgccc acagcagccc attaaattcg attccatcca
      961 ctaactgcta aaagagacta tcgcacgaag gcttctgcca tcatgtgcgc tcccattctc
     1021 tttatagttt atagtatgga gtttgtagtt tacatagaaa gcctttaaat tcaaagccat
     1081 ccactaactg ctaacggcta gctgctaact gctaaaaaaa ggcgctgtct tcgtgcgaca
     1141 gcgccttttt tctgctataa tggatacaaa ttcacttgtc agggggaaag tacatgttcg
     1201 ctgtcatcgt caatgccgtg acgtcgtcat taggggcatt gctgggattt cttttgaagc
     1261 gcggcatacc ggagcgcttt accaaggcca tttttggtgt catttctctg tgcgtcgcca
     1321 tcatgggcat acaaggagcc gtccagagcc agaatctgct cctcgtattg gctagcatga
     1381 ttatcggtac cctcgtcggg acggctatcg gcatcgaaga tggcatgaac cgcttcggcg
     1441 aattcctcaa gaaacgcatg ggccacggcg atgattcgcg gttcgtccgg ggcttcgtca
     1501 ccttatccat catgcaggtc atcggcgcca tggccatcct cggccctatc caggcggccc
     1561 tggggagtca tgaccttctc tatttcaaga gcgccctgga ctttacgtct tctttcattt
     1621 ttgggacctt ttacggcttg ggcgtcgttc ccgtcggcat cgtcctcttc atctatcagg
     1681 gctttttcta tctgttggcg accttcatca tgcccctcat gacgcccgat gtcatccggg
     1741 aatttaatgc cgtcggcagt gtcatgatcc tgggtattgc cctcaacatg ttccagttga
     1801 cgaagctgaa agtagccgat ttcctgccag ccctattcat acctattatt tattatcatt
     1861 taatcgtctg atgatggtat tacgacaatg tgccacttgt catatattcc aatcatttac
     1921 aggattcacg gcgggacgcc ttagacaggc gtcccctacg gcatcgcata caggcgatga
     1981 aaaataacgc ggctatctgc cgcctgcgta ggggccgtcc cggccagggc ggcccgtggt
     2041 gatgttcgac cacagcctat tcgtgtatca tggcaggctc tttaccattt aggaggcgtt
     2101 ttatgcattt gcaggagatc attgatcaat taaaaacgga cgaacggaac gcgtcgtata
     2161 cggcccgcgg catcggtcct atttttcaga tccacgagga agcgagaatc ttgatcatcg
     2221 gtcaggctcc gggcaagaag gtcgaagaat cggggattcc cttccatgac aagtccggtg
     2281 aaaagctgat ggcctggatg ggcattgaca gccagacttt ctattcgccg gccatttcca
     2341 ttatgcctat ggatttctac tatccgggca aagccaaaac gggcgacaaa ccgccgagac
     2401 ggttcatcgc ccaggaatat catcgtgaaa ttctgcaact catgccggac ctggcactga
     2461 ccatcctcgt cggcaagtac gccatcgact attatttgaa aggccggacg gaacgcaacc
     2521 tgacggagac tgtgcggaat tatcaggctt acttgccgga ctatttcccc atcgttcatc
     2581 ccagtccgct caacttccgc tggcaggcca agaatccctg gttcgaggcc gacgtcgtgc
     2641 cggagctcca gaatcgggtc cggtctgtac tcccgtaaat cagccaggca ctacgttttg
     2701 ggaaccgtca aggcatcgag agtcttgaag aagtttttct ttaagtgctg gatgaattct
     2761 tcctttggat agtggacggg cagagccttg atgaccgggc tgttctgggc caggaaggcc
     2821 agggtgaacg gcgcgatgag gctgaggtag gcactgtaaa atccgatatc cgccgtgtca
     2881 tagcccaagg cctgctgcag gacgcctgag gatacgtaga atttgggcag gcccttttgg
     2941 gaaatgatat tctgaatgta cggcgacggg tgcaggagtt cctggatcca gacgcggacg
     3001 tgccagtcgt tgcggcgaaa ggcatcttct ataaaataat cgacaaaacg ttctaatttc
     3061 tggcgcggcg agccttcttg ctgcgccagt tttttgagta cgtccacatt gaccaggtaa
     3121 tcctggactt cttcgaggac ggcgacgtac aggccgtcgc ggctgccgaa gtgatagttg
     3181 acagccgcca tattgacacc agctttttgg cagatttcct tacttgtcgt accgtcgtag
     3241 ccgtgttggg caaagagcgg gcctgcagct tcgatgatgc gtgcttttgt ttcccggccg
     3301 tcttcgcggc tcgacgtcac aggcgcttta tgaggcatag caatccctct ttctatgata
     3361 atttatggcg aaacagccag gcagccaggg gcagggtgac ggcggcaacg gccagcatgg
     3421 gcagataagt ataggcaatt acagggatac cggcgccttc caggtagatg cggcgtatgg
     3481 cttcgatggc aaagcgcatg ggattgatat aggtcacgta ctgcaggacc tgcggcatgt
     3541 tgcggactgg tgtcgccagt cctgacagga gtatcatggg catgaggaat aagaaacagt
     3601 ataccatgac ttgctgcatg ttcttggcta cggctgaaat cgacaggccc aggccgacac
     3661 aacttacgag gaaaatgaaa atcgtaaggt agagagcggc cagggaaccg actaattgta
     3721 ttttaaacca aaacacggcg atgcacaaca tgagcgtcgc ctggacgagg ccgatgagga
     3781 taggcggcac ggctttgccg atgaggattt ctggcggcgt cagcggcgtg accagaagct
     3841 ggtcgaaggt gccattttcc cgttccctgg cgacggagag gccggccagg atgaggacct
     3901 gtgtcaaggc cagcatgggg atgagcgatg gcaggaacat gcggcgtgta atgttattgg
     3961 ggttatacca gatgcgcgaa cggatttcga tgagtggctg atggccgatt cgctggacat
     4021 tcatggctga gacgatagag gcagcataac tggccgccag gccggctgtc atgggattgc
     4081 gtccgtcggt gaccgtctgg atcggcgctt cctggccgct gttcagtcgg cgggcaaagt
     4141 ccgatgggat ggtaatgacg atcatggcct cgccgctgtc gatggcttgg gcgatctgct
     4201 gtgtcgacga caaggtctgg acgcgctgga agatgccggt cccatcgaga tgggcgatga
     4261 aatcggccga ttcccggctg tggtcttcat cgacgagggc atacggtaca ttgtcgaggt
     4321 tatacgtcgc cgtataccca aataggagtc cttggacgag gacggggatg accaggatga
     4381 cacggctctt cgggtctttc aaggtcgtca gcagttcttt gacgatgagc tggtagattt
     4441 tataaaaagt cagtcggaat gtgtccatca ggcaaccccc tttttggtga cgcgcaggga
     4501 caggaaaacg aagaatacgg cataagcgcc gaggatggca cagtccttga tgaccagggg
     4561 ccccgtcgtg ccggccagga acaaggattt catcaattcc atataatacg tcgctggcag
     4621 gacgtggccg atgacggcaa tggctgtcgg tacgctacgc aggtcaaaga ggaaaccact
     4681 gagcatgatc gtcgggagca ggctgacgac cagggccatc tggcaggcca ggaactggtt
     4741 cttggtcagc gacgaaatgg tgaggcccat gccgatggcg acgaagatgt attcaataga
     4801 tgatagaaga atcaaggcca gagacccgtg catgggcacg ccgtagccgt aataagcggc
     4861 gacgaggcag agaacaaggc cgaccagggc gatgcagaaa tagggtatca ttttggcggc
     4921 aatgatttcc agcggccgga cgggtgtgac gaagagggat tccagtgtgc cccgttccca
     4981 ttcccgggcc atgaccaggg aggtcaggaa gacgccgacc agggtcatga cgatgacgat
     5041 gagtccgggg atgaacatcc atgaactcgt attggcgtcg ttgaaccatt gccgcgacac
     5101 cagtgtaatc gtacccgtcc gggacgctgc gccgtggctt ttggcccaag cggcctgcca
     5161 ggcctggaag ccgctggaaa tataggtatt ggccgcattg gccgtcgtcg catcgatgcc
     5221 atacaagagg acctgcaccg tcgcatggcc ttgttccagg cggtccgtga aatccggcgg
     5281 gatgacgatg atggcatcgg cctggcgggc atccatcagg gcggcggcct cacttatgga
     5341 agatacgtag tgcggtgaga aatatggcga gccgtcgagg aagtgaaggg tgctgcgggc
     5401 cgtcggcgag ctgtcttcca gggcgacggc gacgggcagg tccttgacat cgagggatag
     5461 gccggatccc atgaggatga tgagcatgag agggatgacg atgccgatga ggaagctgct
     5521 ggggtcgcgc agcatctgac agaattcttt atgacataag gcgagaaaac gatgcaggtt
     5581 catgacgata cctcctgatg atggcggccg gactcgacga tatggatgaa ggcgtcattc
     5641 atagtcggac tattaccgcc ctgtttccgg atggccgacg gcgtgccgat ggcgatgagc
     5701 tgcccccggt cctgtatcat gatgcggtcg cagtattcgg cttcttccat gaaatgtgtc
     5761 gtgacgatga tcgtcgtccc cttggccgac aggtccgtga tctgccgcca gaagagacgg
     5821 cgggccagcg ggtcgatgcc gcttgtcggt tcgtcgagaa agaggatagg tggttcgtgg
     5881 agcagggccg ctgccatgga caggcgttgt ttgtagccgc caggaaggct gccggccggc
     5941 atatggcgga agccggacaa atcgaattct tcgatgaccg cttcgatgcg ctggtccagc
     6001 cggcggccgc gcaggccgta ggcactgccg aagaagcgga ggttctcttc gacggacagc
     6061 gtcgtgtaga gggagaattt ttgcgctacg tagccgatat gggccctggc ctgggctcgg
     6121 gcggttcgca ggttgacgcc ggctacggaa atctggccgg acgatacggg aagcaggccg
     6181 cagagcatgc ggaacgtcgt cgatttcccg gctccgttcg ggccgaggag gccgaagatt
     6241 tccccttcgt agacgctgaa cgacgtatga ttgacggcga tgaaatcgcc gaagcggcgg
     6301 acaatgtccc ggacttcgat gattttccgt cctgtttcta ctcttttggc agcagtcata
     6361 tcggggcggc tgtagtggga gggaagggac ggttcctgct ggcgcaggag gctcatgaaa
     6421 ctatcttcca gtctggccgg gacggccgtt actgtcaggc cgggcagggc gaggtccttc
     6481 aggctggcct ggggcgtcag actgaaacgg acggccccgc cttcgggaac ggcatcggct
     6541 acggcttccg tttcgtcgat gagggcagcc tgtatgagcc gcggtgggac attttccggc
     6601 ggcctgacga cataacagcg gccctgggca tagtggcaga gcgtgtccgg tgcgccctgg
     6661 gccaggatat gccctttatg caggatgtat acgtcgtggc agtgttcggc ttcttccagg
     6721 taggccgttg cgaccaggat ggtcagggac cccgatgccg acagggtgtc gaggatttgc
     6781 cacagttccc gccgcgatag ggggtcgacg ccgactgtcg gctcgtcgag gagcagcagc
     6841 ggcggcatgg aaacgagggt gcaggccagc cccaatttct gcttcatacc gccagagagc
     6901 ttgccggcca tgcgcttcgt gaacgggctc agaccggtca ggtcgagcag tttcttgaag
     6961 cgctcctggc gcacggcttg agggaccgcg tggaggttgg cgtagaggtc caggttttcc
     7021 tggaccgaca ggtcttcata gagaccgaat tcctggggca tataaccgat atggtcctgg
     7081 acggcctgag gatggctggc cacgtccata cccaggaccg acaggctgcc ggcatcgctt
     7141 ttcaacaggc cgcagacgag gcgcatgaaa gtcgtcttgc cagccccgtc agggccgatg
     7201 atggccgtca gactcccggt gcggacggtc agtgaaaccc ctgataaagc cgtgaccgga
     7261 ctggactgac cagggcggat gaattgtttt acgatcccct gggcctcgag ggctggggag
     7321 gtcgatgcag gctgacagtt cgtcatggga atccttcctt tctgcctcag aaggtgactg
     7381 tcgccggcat gcccaggcgc aggacgttgt cggggtcggt gacgtaaatc cggacttcat
     7441 agacgaggga tgtcctgagg tcatcggttt ccacggattt cggcgtaaac tctgccgtgt
     7501 cggaaatgta accgacctgg cccgtcaagg gcgtgtcgac gctgtcgata gtgaccgttg
     7561 ccgactggcc ttcgtggatt tttcccagtt tatcttccga aatgtaggcc cggatccact
     7621 tggtcgtatc caggctgagc gtatagaccg gtgtcgacgg tgaggccatg tcgcccggtt
     7681 cgacgaggcg cgagcggatg acgccgtcct gcggggcgac gagagtggcc tggctcaggt
     7741 tatactgggc ggtctgcagt tcgttctgca gggactggag ctgtgcctgt gcggcgtcga
     7801 tgtcttcctg gcgatagccg ttgctggcca ggtcgtgggc cgactgagca tgggccaggg
     7861 tagccgctgt cgatttggcc ttggcttccg ctgcatccaa ggtctgcttg ctgatggcgt
     7921 cgccggcata gagcgtactc atgcgctgat aatcggcggc ggcattatcc gcttcggcct
     7981 gagccgattg gacggaagcc gctgcttcgg caatttcttc gggccgcgag ccgttgttca
     8041 tcttagcgac gacggcttcc tgctgggcga tttgcgcctg gcagcgggca atcgtcaatt
     8101 ttaggggcgt cgtattgagc gtagccagga cctgcccttg gtggacacgg tcgccttctt
     8161 cgacattcat cgaggcgatg cgttcactgg cattgaatgt gagcgaaacc tgacggacat
     8221 cgacattgcc atggagcgta agcggcgctg ctttaggctg ctgccagtag caccaggccc
     8281 cagcggcagc ggccaggagg atgatgaaaa tgaagagctg tttcttatgg gcttgcaggg
     8341 aagcggagag catggacatt ccttctttct taggacggta tgcagattag cagaattttc
     8401 tctttctatt atatataatt caaattcaaa tttcaactat aaaatataga aaagatgacc
     8461 atgacatatg taaatttttg acggtaaaac gtacaactaa cttaaaagct tattgacttc
     8521 acgggagcga cacggtataa taggcctcaa caaatacaaa acagtgttga atggaaatag
     8581 taatgtttgg gaaaccatga cagagacctg ctggcggctg agaggcagga tggaatcccg
     8641 gcgtgaagtc gtccaggagc tgctgcccga acgtattttg ctagtagacg cagccggcag
     8701 gcaaccgtta ccttgccagg cattacatgg gctgtcccgg agatgctgga aaagtggtca
     8761 ttacgacaat gagagtggta ccgcagagtg aatactttgt ctctttttag aggcaaagtt
     8821 tttttttgct ccatcaaaac cgtttgcgta tttgtatttg tcaggtctta ttttagaaag
     8881 gatgtgcgtg actatgatgg atttcattgg aaagttgagc ggttttgttg ggaagaattt
     8941 gacgtatatc gtctttctcg tcgtcatctg ggcttatttc atgcccaatg cctttctctg
     9001 ggcggttcct catacggtcc tcttgttagg cgtcatcatg ttcggtatgg gtatgacgct
     9061 ccatgcccgg gatttcaaat tgatcgtcca gcggccgaag gaagtcctca tcggctgtac
     9121 ggctcactat acgattatgc ccctcttggc ctatgccctg gtcctggcct ttgaaatgcc
     9181 gccggaaatc gccgtcggca tgatcctcct cggctcctgt cccagcggga cggcgtcaaa
     9241 tgtcatgggc ttcctggcca aggccgatgt acccctgtcc gtttccatta cgacgtgttc
     9301 gaccttgctg gcgccgatta tgatgccttt tatcgtctgg ggcctggccg gccaatgggt
     9361 cgaagtttcc tttatggcta tggccatgac agtcatgaaa gtcctcctgg gtcccttgat
     9421 cctcggctta ttgacgcatc gcctgatggg agaaaaacat attgcctccc tgtcgaaggt
     9481 cctggtcctc gtttcggctt tcggcgtcct ggtcatcgtc ggcggcgtca ttgccatcaa
     9541 tggcgctgaa atcctggaca tgggtatctt catgatcctc atggtcctcc tgcacaatct
     9601 cggcggcttc ctcatcggct atttcgttac aggcaagctg ggcttgggca agaaacagca
     9661 gcactcggtc acgttggaag tcggcatgca gaacgacgcc ctggccattt cgctggtatc
     9721 ggtcttcttt gccccggccg tcgccattcc ggcagccgtc ggagcggcta tccatcaggt
     9781 aacgggttct atcctggccg gcatctttgc ccgccacatg gatgctcatg aaaccaaaga
     9841 aaaagcgaag gctcaggccg aaacgctcat ggaagccgta ccgggcagtc attgaggtca
     9901 tcgagtaaaa cttgcaagcc ctcatttgtt ttgatactat aagtatagac aaatgggggc
     9961 tttttcatga gatttgttat atatttacga aaacatgtac ataaaatatt tcatgggcca
    10021 tttggtgata tccaggctat tttggcggat ccggaattca ggaaacggaa aatacgggat
    10081 aatctctatg cctgcagttt catttcctgc ctcctgtgca tcatcgccct gaccggggtc
    10141 atcggtacgt atgcgcccaa tgcagccgga gccgtcttgc cggctatcaa tgcaacggca
    10201 gtctatggaa tcatttttag cgtcaatgtc ctcttttttg ccggtttcta ccgatgtctg
    10261 cacggagcgg tccgcaatga aaagatatta gaaaatctgg tctatatctt tttgttcatc
    10321 aattcatccc tggcgtgcct gacttttttc agcacccagg acgggagcag tttcttcttt
    10381 gaatacctgc tcatgctcat catggtctat ttgctgccca tttacgataa cctcaaggtt
    10441 ttgtttccca tcgtcgccgt caatctggtc acgacctttg ccgtcatcgc catgacaggt
    10501 catcagattg cctggcagga ccaatacgat atcgtcctct tttatctggt ttgtctggtc
    10561 atcatctttt cgcggcgcca tctggccctg gcttttgccc agatacgcat gcacctccgg
    10621 tccagtaatg aagagagttt taccagcgga gccggaccga tgagctgacc gggctgctga
    10681 accgcgaagc cctgcgggaa gattttgaac agtatttagg acagacgatt tgtgtcatga
    10741 tttgcgacat cgaccggttt aaatttttca atgatactta tggccatgta ctcggcgatg
    10801 atatcctgtc cggcatcagc cggcttttgt gccatatctt cccggatgat tgtgtctacc
    10861 gctatggagg cgatgaattc ctgattttgt catttcggaa tcaaccttcg ttccaggacg
    10921 atgtagagcg gttccagcgg cacttgaagg acttgccctt tgacgatttg acccgccagc
    10981 cgacgatgag cggcggctat acgtggggcc tctgccaggg cgctgtcgac ctgcggaaaa
    11041 tgttcgcctt agccgaccat ttcctctatc aggctaaaga agccggccgc aaccgcatcc
    11101 actgcgaacc cttcgatgac caaaaagcag aaatcagcct gcatgaaata ggaaacctta
    11161 tagataaaag ttcataaaaa agtagcattc taacgtaggg gctcgcacgt ggcgagcccg
    11221 ccacaagaat gtgtgtcgtt tgcatgaatg aaagtttgtg ggggtcgccc gtgggcgacc
    11281 cgctagggat ttggatggtt ttcctagcaa gctgattggg acatcgtaca tataccggag
    11341 cgggcgcccc acgggtcgcc cctacaacac aaaaaggggc tgtcgcacat gcgacagccc
    11401 ctttttttac gagccactaa ccacgagcca ctaatcccta acctcaaatg acctgcggga
    11461 aggtgttggt cagtatttta gccccgcctt cggtggtttt tgtctgcaag gacgttccaa
    11521 ccgttgcgac gtcttcgata tccgtcggga tgcgcttata gggcagcttt ttcataatgc
    11581 ggtccataga ttccgtcagg atttccttgg ggtcgttcag gatagatgcc gattcgattt
    11641 cacggccttt gtcgctgtag ctcttgaagg tatcggtccg cttatcgtag gcgtataccg
    11701 tcaggtgata gagacattcc gtgatgattt cgtcatcatc atagcgccag agcccggcca
    11761 tgtgctgccg ccagtaccag gtctggacga cggggaccag gacgacgtcg gctttatact
    11821 gggccgaaag ggcgcgcatc gtgtcgatgt tgacgtcaga cggcgacaga gccgtatccg
    11881 tttcgatgcg gtcccagtag gggatgcgga acggctgttt cagccggttt ttcatgaaac
    11941 gggcggcgta gctgtcctgt gtcgtatcgg caatgacgac agtgcgcatg ttattgaagc
    12001 gcgggccgtc ggcaaagacc gttgtaatcg acaggatgca gaagaagagc aaaagacaga
    12061 gttttttcac gaggcaacac ctccagcaaa aaatcagaaa ttgccgttat cgtttaagag
    12121 cggcagcgtg tcgtcgactt gcgggatgta gcgttccaaa gtggccagca ttttttcctt
    12181 atccatcggc agggtgccgc cgacaggcag gagattggaa acgtggttac tgcggaagat
    12241 gcagggcttg gtcatttccg tatggcgcag gatcatgtcc agttcctgga ggaagccccg
    12301 cgctgtcagg ggggtaaacg ttccattgac gacatcctgg tagagcggta cttcgcgggg
    12361 aataatgagc gacaaggcgc tgagcatggt cgggttgatg gccgatacga cctgggccgt
    12421 atgcagggca tgttctttgg tccgttcctg gccgcccaag ccgaggagaa tcatcgtcga
    12481 cagcttcatg cctgctgcca gggctttttt cccggcttcg atggtcaggg ctgccgtcgt
    12541 ccctttatcg acgagtttca agaccccgtc gtctccgctt tcaatgccca tgtagacgat
    12601 ggccaggccc gcttcgcgga ggcgcttcaa ttcttccggc gtcttgcgga ccaggtcagc
    12661 cggagtcgca taggcaccga tacggttcag attggggaat ttttcgtagc atttctgcat
    12721 gatagctacg agtttgtccg tcggcaggac cagggcatcg ccatcagcca ggaaaatccg
    12781 gcggatgaag ggcatggcat aggcgccgcg ctcgatgatc ccttctattt cttccatggg
    12841 acggatgcgg aatttagacg ctttgtacat atcgcagaaa cggcaggtat tgtgggaaca
    12901 gccgatagta acgcgcagga tgaagctgcg ggcttcactg ggcgggcgga agacgaggct
    12961 gccggcatca tcaataatca tagtaacaca cctttctcaa aagatgaaaa aaacattatt
    13021 cattcatctc ttattataca cgaaaagtac gtattacgaa atagctggtt tcttctgcaa
    13081 gatgataatg aagaccgtag cgatgataca ggcatagccg atgagttcaa ccgtatggaa
    13141 cgtcgtaccc aaggcgatgg ccgaagtcac tgaagcgacg accggttcca gcgtgcccag
    13201 gagagtcgcc ttcatcggcc cgatttcact gatacccgtg acaaaagccg taaaagacag
    13261 ggccgtgccg atgatgacga taaaggcaac agccagccag cccgtcaggt ccaatgcggg
    13321 ccagatcgtc cagacctgca gggtcagact gagaaaaacg ccgccgatga tcatgcccag
    13381 gccgttggca acggccgctc cccaatgagc tgccagcccc tgggacagga taggtgacac
    13441 aggcgatggc gccgatgagg ccccaggtca ggcctaacgg agaaatgacc atcgtcgacg
    13501 ggttgccgtt ggtcgcagcc aggtagacgc cgccaaaggc caggaagacc gacaggattt
    13561 cccgccctgt cggccgttta tggaagcgga cggctaaata gagcgacatg aggacgacac
    13621 tcagggtctg caggaccgtc gccgtgccgg cgttcgagtt ctttatggcc gataaatacg
    13681 agaactggct caagatgagg ccgaataggg caaacaggac caagtggcgc agactgaccg
    13741 ggtcatgcag catctggcgt atctcggccc gcttcgtcgg cagtaccatg agcgtcagga
    13801 acaacccggc caggcacatg cgtatcgacg ccagccagga cggctcgaca ggataatgag
    13861 agagcaggaa ctgactgaca acgccattga acccccacgt cgtcgcaccg atgacacaag
    13921 ccacaatacc atgctgcgtt tttgtcatac taagacctcc tctgcctggg gcaatcatat
    13981 tatcctaagt atacacgagg tgaggaaggg gtgcaaggga ataccgcttt caagcggtat
    14041 tccctgtagg agcgccacgt agggcgcccg ctgtcgaaaa aggggcttgt catcatgcga
    14101 ggtccttttc actttatagt ttgtagtatg tagtttatag tttgtagaag atgactttaa
    14161 atttaaaaac ctccgctaac ccctagccac tagctactaa ccactaaaaa aaggcgctgt
    14221 cgcatcgcga cagcgctttt tttatcacag tttcgccaga agactccttc ctcttaggta
    14281 ggggcatcaa tggcgatgga tttcgcaggt tcgatgcctg cgaaatccac cctatgtctt
    14341 ttccgtctta gccttttcac ttcggctacg gtataatggt agataacaga ggaggtgaat
    14401 cccaaatgta cttgacgcaa tcgaatgtca tccgcggctt gtcgaaacaa gattatacca
//
LOCUS       DAWCYS010000020        13909 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_295_length_13909_cov_7.233218, whole genome shotgun sequence.
ACCESSION   DAWCYS010000020 DAWCYS010000000
VERSION     DAWCYS010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_13909_cov_7.233218"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    499..508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3769 a         3558 c         3555 g         3017 t
ORIGIN      
        1 cgtctgcaca cccgttgcaa agtaaccggc cgtcctcacg gttatatgcg taaattcggc
       61 atctgccgca tcacattccg tgaactggct tacaaaggtc agatccctgg cgttaaaaaa
      121 gccagctggt aacacgtatt ccatggaggg aggtttttag attatggcaa tgaccgatcc
      181 gattgcggat atgctcacaa gaatccgtaa tgctaactcg gcatatcacg aaaaagtaga
      241 aatccctgct tcgaagatga aagctgcagt gctcgacatc ctcaaagaag aaggtttcgt
      301 caaaaactac gaatctgtaa acgaaggcaa aacgctgaaa gttacgttga aatatggttc
      361 taataaagaa aaagtaatta ccggcatcaa gagaatttcc aaaccgggcc tgcgcgtata
      421 cgctggcaag gaagaactcc cgctcgttct cggtggtttg gggatcgctg taatttctac
      481 gtcccagggc gttatgagnn nnnnnnnngg gcgaagttag cgcttacctc tcgtaaatcg
      541 ctttggaggt ggcaatatgt cccgagtagg tagaatgcct attgatatcc cggcaggcgt
      601 aaatgtaaac ctcgatggcc acgtaattac cgttaaaggc ccgaaaggcg aactcacacg
      661 tacgctccac caggatatga aaatcacagt ggctgataac gtcatcactg tagaacgtct
      721 gtctgacgat aaaaatcatc gtgcattaca cggcctcacc cgtgccctta ttgccaacat
      781 ggtaaaaggc gtttcggaag gcttcaagaa agaattggaa atcgtcggtg ttggttacag
      841 agcacagatg aaaggtaaga aacttgcctt gactcttggc ttctcccatc ccctcgaact
      901 cgacgctccg gccggcatca ccatcgaatg cccgagcgct acgcagatca ccatttccgg
      961 tgctgataac gaagtcgtcg gcgaatttgc tgctaaagtc cgcggctacc gcctgcctga
     1021 accgtacaag ggcaaaggta tccgctatgt aggcgaacat gtccgtcgta aagctggtaa
     1081 agctggtacg aaaaaataag taacatttaa ttagaaagga gtgaattcct tgaagaagag
     1141 taaaaacaca atccgtttga aacggcattg gcgtttacgc aagaacatca acggcacggc
     1201 agaacgtccg cgtctcaacg ttttccgcag ccttaacaat atctacgctc aggtcatcga
     1261 cgaccagaac ggcgtaactt tggtttccgc aagctctttg gacaaagaaa tcaaagggca
     1321 gaacgtcagc ggcggcaacg ccgaaggcgc aaaattagtc ggtgctttag ttgcaaaacg
     1381 ggctatcgaa aaaggtatca cgacggttgt cttcgaccgt ggcggttaca tttatcacgg
     1441 ccgtgttgca gctctcgcag aagcagctcg tgaagctggc ttgaaattct aatcaaggag
     1501 gaaataacac atggcaaaag ttgaccatgc aggtctcgaa ttacaggaaa atgtagtatt
     1561 catcaaccgc gttgctaagg tagttaaagg cggccgccgt ttttccttca gtgccctcgt
     1621 tgttgtcggt gacggcaatg gcaatgtcgg cgcaggttta ggtaaagctg gcgaagttcc
     1681 tgaagcaatc cgcaaaggcg ttgaagacgc taagaaacat atgatttccg ttcctctcaa
     1741 gaacggttcc attccccaca cgatcacggg tatcttcggt gcaggcaaag tattcctgaa
     1801 accggctgct gaaggtactg gcgtcatcgc tggcggcccg gtccgtgccg tattggaact
     1861 ggcaggcgtc cgcaacatcc tcaccaaatc gatcggttct tccaacccga acaacatggt
     1921 tcaggcaacc ctcgaaggct tgggccgctt gaaggatgtc aacaaagttg ctgaactccg
     1981 tggcaaaact gtagaagaaa tttacggtta ataaggagga tataacaaga tggctcaggt
     2041 taaagttaca ctcgtaaaga gtttggcagg ccgtcctggc gaccagcgcg caaccatcgt
     2101 tgccctcggt ctccacaaga cgaactccag cgtagtaaaa gaagtcaccc cgcagattaa
     2161 agggatgctt cataaagtag aacacttagt aaaagtagaa gaaatttaat atagaaggag
     2221 gtgcgctgaa ttgaaacttc atgaaatgag tattgttcct ggttccaaga aaaaacgcaa
     2281 ccgcgtcggc cgcggcatcg gctccggcaa tggcaaaacg gctggcaaag gtatgaaagg
     2341 tcagctctcc cgtagcggtg gcggcaagcg cccgggcttc gaaggcggcc agatgccgtt
     2401 gtatcgtttg ctcccgaaac gcggcttcaa aaacatttgg gctaaagaat atgcagaagt
     2461 taacgtttct gtactcaact gctttgaaga cggcacgact gtcgatcccg tcgctctcgt
     2521 agaagctggc atcctcaaga atgtccgcga cggcgtccgt atcctcggca acggtgaact
     2581 cgaaaagaaa ctcactgtcg tagccaacgg cttcacgaaa tccgctaaag ctaaaatcga
     2641 agctgcaggc ggtaaggttg tagtaccggg agaagaaccg gtagcagaac agtaggggtg
     2701 agttgcggtg ttagaaaaca tccaaaacgt actgaacgtc ccggaattcc ggaaacgggc
     2761 agtcttcact ctcatcatgt tcatcgtttt cagactcggc gtccatatcc ccgtaccggg
     2821 tgtggattcg tcggtcattg agaatctctt cagcagcggc aacttgttcg gactcctgga
     2881 cttgttcgcc ggcggggcac tgagcaagtt ctccgtcctg gccatgagca tcacgccgta
     2941 catcaacgcg tcgatcatca tgcagctgct gacagccgtc gttccgactt ttgaacaatg
     3001 ggcaaaggac aatcaggaag gccgcgataa gatccagaag gtcacgcggt acggcacggt
     3061 tgtcctggca ctgatccagg ctttcgccat gagctatgcg ctgaaagtca acaatgccct
     3121 catcgtcgac agctgggcca tggtcgtcat ggtctccctg gtcctcacgg caggcacatg
     3181 tttcctcatg tggctcggtg accagattac agctggcggc atcggcaacg gcatttctct
     3241 catcatcttc gccggtatcg tagcgcgctt cccgcaaggc tgcatgacga tttacaagta
     3301 catccaggct ggttcgatca acatcttcca gaccttgctt tttgctgtca tcgccttgtt
     3361 catgatcgtc gtggtcattg aagtcacaca gggtcagcgc cgcgtcacgg tacagtatgc
     3421 gaaacgtatc gtcggccgta agatgtatgg cggtcactcg acacatattc cgctgaaagt
     3481 caatcaggct ggcgtcatcc ccatcatctt cgcttcgtct gtactgatgt tcccgattac
     3541 catcgcacag ttcgtacaga atgaaacagt ccagtggatt gcaagcttct ttgcatgggg
     3601 cacggtgacc aacacggtca tgtacgctct cctgatcatg ttcttcacct atttctacac
     3661 ggctatttcg ctgaacatcc cgcagctcac ggaaaatatg cagaagtatg gcggcttcat
     3721 ccctggcatc cgcccgggcc agccgacagc catgtatatc gatcgcatca tgacgagaat
     3781 caccctcgcc ggtgccttct tcctggcctt catcgccatc ctgccgaact tcatcggctg
     3841 gatcacgggg atcgaagggg tttacttcgg cggtacggcc atcctcatcg tcgtcggtct
     3901 ttccatcgac aagatgaaac aggcagaatc cttagttttg aatagacaat atcaaggatt
     3961 tatgaaataa taagggggta gtttgagtgt atattctttt aatgggccct ccgggtgctg
     4021 gtaaaggtac ccaggctgaa cgattgattg aaaaatatgg tattccccag atttcgacag
     4081 gcgacatgtt ccgggcagct gtaaaagaac agaccccgct cggcctcgaa gccaagaagt
     4141 acatggacaa cggccagctc gtaccggacg ctgttaccgt aggcatcgtc aaagaacgcc
     4201 tcgccaagga tgactgcaaa aaaggcttca tcctcgacgg cttcccgcgc acgaccagcc
     4261 aggctgtatc ccttgatgcc atcctccgcg atttgggcat caaactcgat ggcgtcatca
     4321 acattgccgt ccccgatgaa gaactcatca aacggacgac gggccgtcag atctgccgct
     4381 cctgcggtgc gacataccat gtcacattca aaccgtccaa ggtgaagaac gtctgcgata
     4441 aatgcggcgg cgaattgtat cagcgcgatg acgataaagt agaaaccgtc aagaaacgct
     4501 tgagcgtcta cgctgcacag acgaaaccgc tcatcgacta ctataagaat tccaatctct
     4561 acatcgaaat cgatggcaaa cagagcatgg aagatgtcta caaagacatc gttgctagtc
     4621 ttgaaaagag taaataagca tgattatttt aaaatccaaa cgggaaattg agtatattcg
     4681 cgaagctggc cgcattacgg ccaacgccct tcagctcatg aagaaactgg caaaacctgg
     4741 catcacgaca ggcgaactcg accagcgctg tgaagaatac atccgcagct gcggcgcttt
     4801 tccgagttgc aaaggctatt acgggttccc ggcaaccatt tgcgccagcg taaatgaaga
     4861 agttgtccac ggtatcccgg ggcaccgcaa gctcaaagat ggcgatataa tcagcattga
     4921 tttagtagtg aacaagaatg gctaccatgg cgattcgacg gtaacgattc ccgtcggcca
     4981 cgttcatccg gcgaccctca agctcttgaa ggtcaccgaa gaaagcctct ataaaggcat
     5041 cgaacaggcc gttgccggga atcacctcgg cgacatcggc cacgcagtcc agtcgtatgc
     5101 cgaatccttt ggatacggcg tcgtccgcga ctacgtcggc cacggcatcg gtacggacat
     5161 gcacgaagat ccggaagtgc cgaactacgg catggccggt cacggcgaac tgctggaacc
     5221 gggcatggtc ctggccattg aaccgatgat caatatgggc acggaaaaag tccgccagtt
     5281 gaagaacggc tggaccgtcg tcacgcgtga cggcaaaccg gcagcccact tcgaacatac
     5341 agtagccatc acggaaaacg gtcctgaaat ccttaccctt ccggaagaat aagatggaac
     5401 ggacacagat gatacctgtc tgcacggtcg tagaaagcct ggcaggcagg gataaaggcc
     5461 acttgtatgt tgttgtcggc agtctggctt atccctacgt atggattgct gacggtagaa
     5521 agtacaacct ggataagccg aaaaaaaaga attgccggca tctccggata ttgggaacga
     5581 gcgtatccac tggaagcgct ggtcccgcga gaatcagcaa tgaatggata cggtccgtct
     5641 tgaaccgggc tgtgaagaat tgaccaggga ggttactatg tcgaaggtag atgtcattga
     5701 agtagaaggc gtcgtcgttg aagctttgcc aaacgccatg tttaaagtca agcttgaaaa
     5761 cgatcacatc gtactggctc atgtctccgg taaaatgcgg atgaacttca ttcgtatcct
     5821 tccgggcgac cgggtcacac tcgaattgac accgtatgat ctgaatcgcg gtcgtatcac
     5881 gtaccgcttt aagtaagagt attacgcagg aggttaatga tgaaagtaaa accgtcagta
     5941 aaaagaattt gcgaaaaatg caaaattatc aaacgtaaag gccgcgtcat ggtcatttgc
     6001 gaaaatccga aacataaaca gaaacagggt taataagcag gaggtgaatg aatagatggc
     6061 acgtatagcc ggtgtggatt taccacgtga taaacgagtt gaaattggtt tgacttacat
     6121 tttaggtatc ggtctttctt cttcccagaa gatcttggct aagactggcg taaacccgga
     6181 tacccgcgtt cgcgacctga cagaagacga agtacagaaa attcgtgaag tcatcggttc
     6241 tgaatataaa gttgaaggcg aacttcgccg tgaaacagca ctcaacatca aacgcctgat
     6301 cgaagttaat tcgtatcgcg gcaaacgtca tcgtgcaggt cttccggtcc gcggtcagcg
     6361 caccaagacg aacgcacgta cccgtaaagg tccgagaaaa gctgtagcag gtaagaagaa
     6421 ataagattct ttaagtaagg agggaaacga ttttggctaa gaacgtaaga agcaacaaaa
     6481 gaaaagaaaa gaaacacgta gaatcgggcg cagcacatat ccgttctact ttcaataaca
     6541 cgatcgttac gatcactgat gcaaaaggca atgcactgtc ctgggcaagt gccggcggcc
     6601 tcggtttccg cggttcccgt aagagcactc cgttcgctgc acagatggct gctgaacagg
     6661 ctgccaaagc tgctatggaa catggcctcc gtcaggtaga agttttcgta aaaggtccgg
     6721 gatccggccg tgaagctgct atccgcgcat tgcaggctgc aggtcttgaa gtcaactcca
     6781 tcaaagacgt aactcctatt ccgcataacg gctgccgtcc gccgaaacgg agacgcgtat
     6841 aatcgtagat aattaaccaa ggtgttttga ttctgaaata ggaggatttt cctaatgatc
     6901 gatgataaca agtttaaagt taaaaccgtc gaaatcaatg acggcggtac ctatggaaaa
     6961 ttcgtatgtg aacctctcga tcggggttac ggaatcacat tgggtaatag tttgcggcgt
     7021 gtcctcctgg catccctgga tggcgtagca atcacttcga tcaaaatcga tggtgttctc
     7081 catgaatttt cgaccatccc cggcgttcgg gaagatgttt cggaaatcat cctcaacctc
     7141 aaacaggttc ggatgaaatt cctggacgat gatatcaacg aactcgatat cactgtcgat
     7201 atctctggtg aatgtgaatt cactgcaaac gatcttaacg taaactcaga cattgaaatt
     7261 ttgaacccgg atcttcatat tgccacgctg gatacggacg ctgaaatccg tatgcagtgc
     7321 cacattgaac gcggcaaagg gtatgttcct tctacgaaga acaagaaaga aacggatatg
     7381 atcggggtca tccccatcga ttcgattttt tcgcctattg tgcgcaccaa ctacgaagta
     7441 ggcaatatcc gggtcggcaa cgaaatggat cacgattcgt tgacgcttga agtgacgacg
     7501 gacggcagca tcagtgctga aaacgccgtc gccaaggctg catccattat gatcagctat
     7561 ttgaattact tccaggacat cgccaccgac gccaaagtca atgacacgtt ggctgaattc
     7621 accaacaaag atggcgaaga accggaagca ccgacagaac cggacctcaa cgacatcaag
     7681 attgatgtcc tcgatctttc cgtacgcgct tcgaactgcc tgaaacgtgc aaacatctac
     7741 accttaggcg acctcgtaga acggacggaa gacgacttgt ccaagattcg caacctcggc
     7801 aagaagtctg tcgatgaaat cattgaaaag ttgcaaaaag aaggttacga tttaaaatcg
     7861 agtaacgaag aatagttcga ggaggatgaa cgatggctta tagaaagttg ggacgagaca
     7921 gctccgcacg taaagcattg ctgcgcagca ttgtcacctc tttattccag catgaacgca
     7981 ttgaaacgac agaagcaaag gcaaaagaac ttcgcaaagt cgctgacaaa atgcttaccc
     8041 tcgcaaaacg cggcgacctc cccgctcgcc gtcaggtctt ggcatacatg atggatgaag
     8101 acgttgtcaa aaaactgttt gacgaaattg caccgaaata caaagatcgt cagggcggtt
     8161 acactcgcat catcaaaact ggcgttcgtc agggcgatgc tgctccgatg gtaatcatcg
     8221 aattagtata agcttgtaag aaaaaagggg ctgtcgcaag acagctcctt ttttgcacaa
     8281 aggagtgata caaatgccat atcgtatgat gttagctgtc aactttacac cggaacggct
     8341 ggaccaggtc aagctcctgg ccctcttgac gaagtcgaag gtcaaggctg ttacggaagc
     8401 ggaaaaaggc gaaaccgtcg gcaaagtcct gggactgact gacgaagaaa tcgctgaaat
     8461 cgccgctatg aaacaggaaa cggaacagac caaggtccat gaacagatga acgaagacga
     8521 aaaacttccg cctgtcacca aagaagcgct gattctctgc ggcttcgata accaggctgt
     8581 caacctgctc ctgagcagca tccgccgggg ccgcctgaag aacgtgcccc tcaaggccat
     8641 gttgacgccg aacaacattt cctggactat cgaaacgatt ttgcaggaat tgtcgaaaga
     8701 acacgaatat tttcataaaa agaggtaaga ccatgcgtcc tacgacactt tgctttcccg
     8761 tccgcccgga cgggaaactt ttattaggcc gcaaaaaacg cggcttcggc gtcagcaaat
     8821 ggaacggctt cggcggtaag atcgaagccg gtgaaaactt cctgcagtgc gccgtccggg
     8881 aactccggga agaaacgggc ctcatcgcca gggaagagga cctggaactc gtcggcttcc
     8941 tggacttcca cttctcggcc tcaccggacc tggaccacat cggcttcgtc tacttcctcc
     9001 gccaatggca ggggactgtc caggaaaccg aagagatgga accgcaatgg ttcgacccgt
     9061 cagcccttcc gtatgatgaa atgtggaaag gcgaccggac ctggattcct atgctgctga
     9121 aaaaagaaaa ggtcaaaggc atcattacct ttgccgaaga taatgagaat ctgcaacatc
     9181 tagaattgta tcgcatagac aatgtataga ttcatgagtt agctaagacg acagatggac
     9241 aatatctgtc gtcttttgca gtcttgagac aattgcgaca aaatgcgggt cttgacattg
     9301 ttttggcatt tggtttacta cttataaagt attaataggt gataaataga acgtaatcat
     9361 ttttaaaact ttatgtatgc aatagtaata atgcatatta attatgcttt ttattttatg
     9421 atgaagtgga gtttgatgac gatgaaaacg atgaagacga ccttgatgag tgccatctta
     9481 atgagtttgg gtatgacggc gagtgtattg gcagcaccgg cgacgacggc caacgtttcc
     9541 gtagacagcc cgtattatgg ctatatcgaa aaattgagtg ccatgggcta tctcgatacc
     9601 atgcccaatg gggccaaacc atacagccgc atgcagatgg ctcaatgggt cgtccaggct
     9661 caggataagg cccagaccaa acccatgccg aaatacttgg cagatcaggt cgatgccctg
     9721 gcccagtacg tcgccccgga agtggctacc ctgcgcgggg aaaagaccta cgacccgctg
     9781 aaactccgct cggtctcgct gacggcagca gcacagctct cagatacgcc gcgccattcc
     9841 tatagccgtg ctgtcaatgc gggctggcag acttttgggg ccaatcgcaa tggctataaa
     9901 tacggccgtg acggcaatgg catcttagaa gctgaaattt ccgggaatat cggccatgaa
     9961 acggctatcg ccctgcggcc gcgcttcagc tatgacaagg acaacgactt cagtgccagc
    10021 ctggaagaag gttatatcaa gacccgtgcc ggtatctggg cctttgaagc cggcaaagaa
    10081 gccatgacct gggggcaggg ggaaacgggc aatctggcct tagggaacaa catgcgtccc
    10141 ttgacgacca tccaggcgca cttcattgaa ccgcagaaag tcggcggctt cttccgtttc
    10201 ctcggtcagg cagacgtcca ccttttctac ggtttccttg aaggcgaccg gcgtgatcgt
    10261 gcggctgctc taggttcgaa gtataaagat tctgacgatg ccggtctcat cggcatccgc
    10321 gctgactttt cgccgacatc atacttcacc ttcggcctgt cccgcctgtc catgctcggc
    10381 ggtgacggca atggtctgga tagctctgac tggggccatt ggctttatgg ccgcaatgac
    10441 gatgccgata aggaccgctg ggatgacatt gccggcggcg acttccgcct gagcctgcct
    10501 ggcgtcacct tctatggcga actctacggc gaagaccagt cgcattatat gccgtccaaa
    10561 gtcgcatacc gggccggtat ctacctgccg aaactgactc atgacgggtc ctgggacatg
    10621 accttggaaa tggccgatac cagcgatgcc tggtacggtc accagcgctt caataacggc
    10681 tggacctatc acgacgccat catgggcgat gccatgggcc gcgatgcccg caaatactac
    10741 ggcgccatcc gccattattt gcccaacgaa acatccatcg gcctctatgc ccagcggacc
    10801 gaaatggaac ggggcatgcg catccatcct acagtcaatg aattcggcct caccggccag
    10861 accaaacttg cccaggacgt ctacttaagc ggcatcatcg gctacgccaa cgtagaaaac
    10921 gccgacttca ccattcatac agaccacgac aaattcgcca ccgccaccat ccagtggagg
    10981 tattagaata cggctctctt gataggccct ttttggcccg tcatggactg accggaggga
    11041 agtgcatgac ggttgccaaa acggagagcc gtgctagagt tgtcagcgaa gctgactgag
    11101 aggtttcgca ggccggcgcc cacgaccaac caacctctcc ggcctttcag gccacctccc
    11161 ctataaaggg aggcataacg acgaacgatc tacgatgaac gacaggggcc ccggccccca
    11221 gacggagaac tatcatgata gacattcact cacatatttt atacgacatt gatgatggct
    11281 ctgaatcgct ggacatgtcg ctgcagatgc tgcgcatgtc cgtcgattcc ggggtcaccg
    11341 atattattgc gacgcctcat gtcaatcgtc agggccatat accggcctgg cagacgattc
    11401 tcgacaaggt agcagccctt caggaagaag cggataaggc tcagattcct attcgtatct
    11461 atacgggcgc ggaagtggaa ctcaacggcg atacgctgcg ctttatgaag gaaaatcacg
    11521 acgattattg tctggctggc agccgctatg tcatgttgga actgaccgac cagtcccagc
    11581 cggatatcgt cgaaaagatg atttatgagc tgcaaatgcg cgggttcctg ccacttctgg
    11641 cccacgtaga acgctatgag cgcctcatgg agcgtccgga aacgctcttg gcatggatga
    11701 ataagggcgt cctggctcaa tgcaatacag gcagtttcac gggctatttc ggctcgaaat
    11761 tgcagcagcg cgtccgcgac ctttaccgca atggcatgat ccacttcctc ggcagcgacg
    11821 ggcatcgcgt ggaattccgc acgacggata tccgcgaagc ccacgaagaa ctggatcgcc
    11881 tggccgccaa ggataagccg catgtcaatc tttggaatat ggcgacgcgc aatgccgaat
    11941 atatcttgaa aaaccgcgtc ttttatcccg atttgccgtc gcactggcaa aaggagaaaa
    12001 aaggcttttt ccatcgtctc tttggttgaa cataggatgt gaatacagat gttaaagaaa
    12061 agtatactgg cattggctgc cgtgaccgcc ttgtggaccg ggacggcctg tgctgaagag
    12121 tattatatgc ggcctggcga tgagctcaac atcgtcgtca cgcagcagca ggacctgggc
    12181 aattcgtcga ccaatcagtc gcccttcgtt gtccggcccg atggcaacgt gtcctttccc
    12241 ttagtcgggg aaatccacgc cgaaggcatg acggtctctc agtttaccaa tgtcctgcag
    12301 cagggcttgt cccgctatat cgtcgatccc gatgtatccg tcaatatttc caagttaggc
    12361 cgcgtccggg tctacgtctt tggcgaagtc cgcaaacctg gagctgtgga attagataaa
    12421 ggccataccg tcatcgacgc catcggcgca gctcagggct ttacgcggga tacggcgaag
    12481 aagaagattt tcctcatcca tcaggaccag ccgaaatcgc tcattcccat taacctcaat
    12541 aatatgctca aaaccggcga tatgtcgcag aatgtcactc tccgtgaagg ggacatcctt
    12601 tacctgacca agaatcatcg catcgacttt gcccgcgata ttgcgccgat cttcagcagt
    12661 atttacatga ttaccgaagc gaaagacaac ttagataaca attagtattt accatacagg
    12721 agttgaacag ggtggaagaa ataaccattg atttgaagga cctctttcaa gtcgtcatgg
    12781 acaataaaaa gaagattgca gccatcacgg cggccttcat gattgccggc ggggcctatc
    12841 tttgcattgc ttcgccagtc taccagtcga cgtcgctgct gcgcatcaag caggataatg
    12901 gcttgggcga ctcgttaatg tctaaggtgt ccgggggcaa taccctggat gataagcagc
    12961 gcatgatgac cgatgcagaa atcctcaaga gccgtaatgt cgtcctgccg gtcatcgaaa
    13021 agacggaaga acgcgacgac gatggcgaat tgccagatta tgaaagctat gtcaaaaaac
    13081 atatcgtcac taagccgtat aaagatacag aaatcttgga agtcgacgtc accggcaagt
    13141 cgccggaaca ggcccagaaa gccaataacc tcatcatcaa gggctttatg aaccgcctga
    13201 ccgaactgag ccatgacgaa cagaagcgga cccgcgagtt cctggaaaag aggctggccg
    13261 cgtccaaaga cgaattgagc caggccgaag ataagctgca gcagtatcag gccgccaaca
    13321 agatgtactc aacggacgac cagatgaagc agctcacgga taaattgaac atcgtcgaca
    13381 aggccaaggc tgaaaatcag cttaacttgg aaacggccca ggccggcctg agcagcgtca
    13441 atgaacagct cagctcagct ggcgtctcca tcgccgacag cccggctatc cagcagtata
    13501 agacccagct ggcccagttg gaagcgacca aggccagcta cgtcggcaaa tatacggacg
    13561 aacatccgaa gatgcaggaa atcaacaacc agatcaattc cacccgggat tccctgaatc
    13621 aggaaatcag caagatcgtc gcccagcagg cgccgtccag caacagcgtc cagcagggct
    13681 tactggccga taaattcaag aatgaagcgg ccatcgccgt cgctcagagc aagaagacgg
    13741 ccttggacca gatggatgcc cagaatgacg ccatcatcgc gtcgctgcca gaaaaagaac
    13801 agggcttcgt tcgcgtcaag cgcgatgccg acgtgtccaa cgaaatctac gtcatgctgg
    13861 ctaaacgcct ggaagaagcc aaggtcgccg aagttatggt gccgaatga
//
LOCUS       DAWCYS010000021        13652 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_302_length_13652_cov_11.691035, whole genome shotgun sequence.
ACCESSION   DAWCYS010000021 DAWCYS010000000
VERSION     DAWCYS010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_302_length_13652_cov_11.691035"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3353 a         3678 c         3467 g         3154 t
ORIGIN      
        1 cgggctcgcc acgtgcgagc ccctacatgt ttttataccc attttcgtag gggcgtccac
       61 gtggggcgtc cgttccacac cgatcggcca tgtcctattc acggttaatg gggccgtttt
      121 gcaccaatca tgcggattga tgcaggtggc atcaacgcag gccgcagcgt aggggcgccc
      181 acgtggggcg cccgttccac atttatcggc catgtcctat tcacgattaa tggggttgtt
      241 ttgcaccaat catacggatt gatgcagggg tcattaacgc aggtcgcagg ccacagcgtt
      301 cccataggac cgcacgtaag ggccgtccca aagggcggcc cgcttgtggg aatcccacaa
      361 ccaacatcat gcgttctgtt ggaataccgc cagttgttcc cgcgtcggca atccttcgtt
      421 atcaccggcg acctggatgg ccatggcccc aatggcagca ccctggcggg cagcatcttc
      481 caggctcagt ccatcgagga tggcactggt gaaaccgacg gcaaagccgt caccggcgcc
      541 gacagtatcg acgacgtggt cgacgtgata gctcggcacg cggtattcct ggtcatcggc
      601 cgtgcggata taggtcccat ggctgccgcc cattttgatg acgacggtct ggacgccgcg
      661 ctgcaaataa taaccggcga tatctttttc attatccatt cccgtcagga tccggccttc
      721 attgagacca ggcatgacga tgtcggcata ctgggcggcg tcgttgatga cacgggccat
      781 gatttcctgg gacttccaga gcatgggccg caggttgggg tcaaaggaaa tgcgcacgcc
      841 cttaccgtga gcccgcatca tcagcgtata aatcgtttcc cggcacgatt ccgacagggc
      901 cagaggaatc cccgtgacgt gcaggtggtc gacgccggtc caatcgatac cggagaggtc
      961 cgccggtcgg aagtgggaaa aggccgtgtg tttgcggata ttggctaccg tcgggtcccc
     1021 ttccaggacc ttttctttca tctggaaccc cgtcatatgg tcatcatcgg tccagacata
     1081 gtcatcgtgg atgccattgg ccttgaggaa gttgcagatg cacttcccga aggggtcgac
     1141 gccgaccttg gaaatatacg acacatcatt gcccagccgg gccatgccga cggcgtaatt
     1201 cagttccgcc ccgcagacat ggcgggaaaa atgttccact tcttccagcg gccccatttc
     1261 ttcagcaatc agcaagccca tggcttcgcc gatagtaagt actttactca tagtaatatc
     1321 accctttata aaaatgagcc gccaactaaa gcaactcatc tagatataaa cttatttctt
     1381 caacggcaat ggttcaacac gtccaaccaa gaagagatac gataaggccc ccaaaataca
     1441 tagaatacca gcaacagcta acggaactaa gaaggaacct tgcgtgattg tcaacataac
     1501 acctgtaaat gtagtaataa atatgccagc gagattccct gcaaaattca taataccacc
     1561 aatagaagca acatattttt gtgtcggtgc aatttcacca acaataaccc aggtattaga
     1621 tcctgcaaaa gataaactag cataagataa tgcaaatagt gctaaacaaa catatacatt
     1681 atcaacaata gcacaaagtg caatacagga agaaagaaac attccaccaa ctaaacaaac
     1741 tttacgagaa aaagtagctg tgtgtccatg gcgtaaaagc cagtcagacg taaaaccacc
     1801 aagccatcca cctggaattg ctaataatgc cggtaacatc cccaatgtcc ccaatgcttt
     1861 tagagaaaat ccacgtgttt ccatcagata gcttgggaac catgtaataa agaaataaat
     1921 ggcaaaattc aaacaaaata atcctatcat taatccccat acagtccgat agcgaaacag
     1981 ggctgcccaa gaaactttag attgattttt agctgattct gcctcttgtt ctcctcgttc
     2041 agctaataaa gcttcaactt gtccaggagc aatatcacga tacttttcag ggtcatgata
     2101 aataaaccac catgctaaag cccaaataag accaataata cccgtgataa taaaggagcc
     2161 acgccaacca agaatagaaa taatccaagc aatcaagggc aacgataagg cagaaccaac
     2221 tctagaaccg ctatcaaaaa tactgcaagc aatgccgcgt tctttatgag gaaaccaatt
     2281 ataagcagct ttggcaaaag acggcatagc tgccgattca ccaacaccaa gcaataatcg
     2341 cgacccaacc atatgaccaa aagtatgaac catccctgtt actgcggtaa agatagacca
     2401 ccaagcaaca gcaaaagcca agcttacgcg aacaccgact ttatcaataa accacccggc
     2461 cggcatttga cataaagcat atgtccaaaa gaaagcactc ataataaccc ccatcgttgt
     2521 cgggtctaag gccaattcag cccgaatatg aggtgctgca atagctaaat tagcacgatc
     2581 aataaagtta atcgcattcg ctaaaaagca tagtccaata attacccagc gcatattttt
     2641 aaatctttct gccgtcatcg taagttcctc ctttttataa ctgcccgcac gtgcggttaa
     2701 tagcatccca taagccacag atatagttag caccaagggc ccggtcgtag aggccatagc
     2761 ccggacggcc ttcttcgccc cagatcatgc ggccgtggtc cggacggata gggccgtcga
     2821 acccgatgtc ataagcggct ttgacgattt cgtacatatc gaggttgccg atttgcgatt
     2881 tatgagcgac ttcgttgaag acccacggtt catggacctg gatgttgcgg atatgcagga
     2941 agttcacgcg gcccattttc ccgaacttgc ggatgatgtg gggaatgtcg ttctgacggt
     3001 tcgagccgag ggaaccggtg caaatggtga agccgttgct cgggctgtcg accatggctg
     3061 cgattttgtc gaggtcgtct tcgtttttga cgatacgcgg caggccaaag acggaaatcg
     3121 gcggatcatc gggatgaatg gccatcttga tgccgacttc ttcagcgaca ggaacgatgg
     3181 ctttgaggaa atattccagg ttcttgcgca ggccgtcttc atccatgcct tcatactgtt
     3241 caaacaggtg cgacaattct ttcagccgtt ccggttccca gccgggaagg acgaagccat
     3301 tgctgttgtc gaggatttcc cgggccatgt cctgcggcgt cttgccggcg atgagatggt
     3361 agtcataagc caggaccgtc gagccgtcgt aaaggggttt ggccaggtcg gtccgggtcc
     3421 agtcgaagac gggcatgaaa ttgtagcaga ccatctggat gccggctttg gcagcattgc
     3481 gcagggacgt gatgtaattt tcaatatact ggtcacggct gggcaggccc aatttgatgt
     3541 cttcatggac gttgatgctt tccagatcct tgaagaccag gttgttcgct tcagcctgct
     3601 ttttgatgtg aagcagcggt tctactggcc atacttcgcc gacaggaatg tcgaataaag
     3661 cgccgacgat accgtccata ccaggaatct ggtcgatctg ctgcaaggta attttgtcat
     3721 cattttctcc gtaccaacgg aaggtcattc tcataaggca atcctccaat tataaatgaa
     3781 ttataaaata tttgttttct ttgatgtttc ttttatttct tttcgcagtt acatactacc
     3841 atactagtat ccattaagca aggtaagaca ttataatttg aataagtaga atagaaaaat
     3901 tgaatccatg atttttgtgc ataaaaaggc atgaaaatgg ccaaaaaaag cgggccgccc
     3961 tttgggcggc ccctacgatt gcggtctgca aaccgcggcc tgcaggcttg ctaccaggca
     4021 agccctatat cattataaaa tgacgttctt ctgatttccg gctaagtatg cgtcgatgtt
     4081 gtcaaagacg atgacggcgc gtttcgccat ggattctgcc gtagcgaaag cgacgtgcgg
     4141 cgtaacgata gtgttcttgg aatggagcag cgggtgattg gtgtcgagag gcggttcgtt
     4201 ttcaaaaacg tcgatgccgg caccggcgat tttaccgctg ttcaatgcat cagccaaatc
     4261 ctgggatacg acgacaggac cgcgggcttc gttgatgagg aaggccgtcg gtttcatgta
     4321 cgacaaggtt tctttattga tcaagccctt cgatttgtcc gtaaccgggc aatggaggct
     4381 gacgatatcg gcctgttcca tcatttcttt catgggcagg tatttaatca gttccgtatc
     4441 ggctttatgg gagaagccgt tataggcgat gaccttggcg ccaaaggcat ggaccagttc
     4501 ggcgacgcga tggccgatat gaccggtacc gacgagggcg acggtcttgc cttccagttc
     4561 gttgccgacg aggccggctt tcgtaccgcc agcacggcag gctgcatcga cctgaggtac
     4621 gcggcggagc aaatcgagca tcatggcgat ggtcaattca gctacggcaa ctgtcgaata
     4681 gccggacgca ttgctgactt tgatgccttt ggctttggcc gcatcgagag cgacgtggtc
     4741 gacaccggta aaggcgacgt cgatgtactt caagttcggg caggcattga tgacttcggc
     4801 tttgaggggc atattggcga tgatgaggat atcggcgtcc ttggcttctt caatctggac
     4861 ggccgggtca tcattgcgtt catacgcagc gaattcatag cctttttctt tcaagggttt
     4921 cacatacgaa tcaagtaatt cctggctgat acctaacgat tccaacaaga caattttcat
     4981 ttgtaaaatc tcctttcagc ataggtaata ttttgattta attatacgca tatattggca
     5041 atttgtaagc tatctatggg aaatcagtat aggtataccc tctatatgat aaaaacatat
     5101 actatttttc gtctaaggcc gcttgtactt tagcacatag gctcaataat agttttcgtt
     5161 cctctggcga caggccttgc agagcccgtc gccgatgagc tgtcacaaag ggttctgcct
     5221 gggcgaacaa gtcctggccg gatgctgtca attcgatata ctttccccgc cccttttcca
     5281 taggatggcg gctaatgagg cctttctttt ccagccggct caaagtgcgc gtaatcgcag
     5341 ccgcttccac cttgaaatag cggatcaact cagcctgggc aatgccgtcg ttcaaatgga
     5401 tgaagttcag gaccatccat tccgagctgt gaagtcccaa aggcgctaca cagccattta
     5461 aagtccggga aaagatacgg gtcgtgcaaa ataactgccg cactagttca tcatcttgcc
     5521 ggtcttgtat atccataatt gtaatacctc tcaatcttca tggatatcgg cgacagctcc
     5581 tttccccgct ttctggctgg ccatgacatc gctgccataa cgataatgac agaacatgaa
     5641 caggacagca aagcaaatca tgagagctgt aacgctccag tacatgtggc ggtagcctac
     5701 catgccggcg atgacgccta agacgaggat acccgtgctg gtgccgatat caagcatatt
     5761 atagaaggtc gcgctggcag aactgcgccg ttccgggcgg accatattca aaatccacgt
     5821 cagcagggcc ggaagcagca ggcctgcacc taagccataa aagacggacg ctgccaataa
     5881 gaccgtgagc gaatgagctg ccatgatgag caataagccg atcaagtaca agaccgatcc
     5941 gggcatgacg acggcaaaag cacctttagc atcgaggaga cggccgccaa aaggacggaa
     6001 aatgaagacg aacagcgtcc cgatgatgaa gaacagaccg gcattttcga tatgcgcttc
     6061 agcagccatc aaggcgatat acgtattgac actgccatag gcggcaccga agaaaatcgt
     6121 caaaacagcg ggaaggcccg taccggtttc acagatgcgg tcgcgcagag aagccttttc
     6181 cttcacttct acccgacgaa ctttcttgcc ggcatgacag aagacactgg caaggacagc
     6241 caggaaggaa acggcggccg aggcaaagaa cagggctgct gggctgatgt cattgagtaa
     6301 aaataaacca acagccggag ccagggccat catgaccgta ctgcccaggc cgaaataacc
     6361 aatcccttcg ccgcggcggc tggccgggat gacatcggcg acgatagctg ccgcaaaggt
     6421 cgtactcagg ccaaaaccga agccgtggag gatgcgggca gctacgatgg cgtggatact
     6481 atcaaacata taatacgaca cggtagccaa aatggaaagg gccaggccca ggtaaaggca
     6541 cttctttttc cctaaatagt ggacacccgt atcagtgaac agacggatac agatggccga
     6601 ataaccaaag ataccgccga tgaggccgat ttccccaccc gtagccccta aactggccgc
     6661 atagagggta atcgtcggca agaggaaatg aaacccggca aacaaaaaac cgttggccag
     6721 gaccagagcc atgaaatcac gagtccataa tctttctttt atcactgcaa tcgctccttt
     6781 tataattgac tagtcaaatt tgttgacgga atctatcata acactgttat attgactagt
     6841 caatactatg tgaggatttc tgccatcatg ccggacgctt ttatctcaaa agttgtccgt
     6901 tccggcagct gtcttgagtt catttccacc gcctgtatgc gatatcgtag gggacgtcct
     6961 gctaagcctt tttggcccgt caggtgccga ccagcaggag gcgcaggacg gctgccaaaa
     7021 cggacagctg tggctaggcg tcccgctgtg aattgtagac atagctattc gggcagatga
     7081 aggatgtttt cacgcatcac ccgtcatgca ttacgtgtac accaccatga cagacgggcc
     7141 gccctttggg acggccccta caaagtccat aatacaaact ctatcacagt gtcttcaact
     7201 tgaggtgcgt cgccttgagg agttcttcca tggacgaagc gccgacgatt tctgcggctt
     7261 tgaggacgat gtaggcacag gcgcgggcgt cgtccaaggc ctgatggtga tggaaggaat
     7321 agcccaggtg accggcaacg gtgtccagtt tatgattggg caagtccggc cagacgcggc
     7381 gggagatttc gacagtgcag gcataggacg cctggggctt ttccaggtga tacgtcttca
     7441 gcaggctgcg caggacccgc gtatcgaaga cggcatagtg ggcgaccagg atgcgtcctt
     7501 ccagatgagg gcggatgcgg tcccacaggg catcgaactt tggtttatgg gcgacctggt
     7561 ccggcgtgat gccatggatc tggatattgc ctgaatcaaa aaccatgaaa ggcggtcgga
     7621 tgagggtata gccttctttg acacagcggt cgtcattcat ggtgaccgca gccagggagc
     7681 aggcactgtt ggcgtattta ttggccgttt caaagtcgat agccgtaaaa tcgagcataa
     7741 tctaccttcc tttttgacaa acgaacgaat tctatttaca atagctacaa atcttatttc
     7801 tgcgaggtat tcccatgacg agcgaacaac accagttcac ggcccagggc cggaccttcc
     7861 cggtcatcct ggtccgcaag aaagtgaaaa acatcaatct ccacgtccgc agcgacggca
     7921 cgctgtacct gtcggcaccg gcccgcgtcc cctgggctta tatcgaggat ttcctggaaa
     7981 agaagacgga cttcatcatc cgcgaaatgg aagaacggaa acagaaattc cccatactga
     8041 ccctcagcga cggcgacacg ctctatctgg caggccagcc ctacaggctc gacgtccgcc
     8101 tgggcctgca caacagcatc cgccgcagcg gccagacggt cttcatggaa ctggccgatg
     8161 acacgccggt catgcggcag aagctctatc ataagctgct gcaagccctg gggaagaaac
     8221 tctttcctgc cagtttgtcc cgtatgcagc ccttattcgc cggcctcgcc ctgcccgacc
     8281 cggtcctgaa acagcgggtc atgcggtcgc gctgggggtc ctgcatgccc ctcaaaggca
     8341 tcgtcaccat gaacacctac ctggccatca tgcctgaagc catcatcgac cacgtcatgc
     8401 tccatgagct ctgccatttt ctccagccca atcattcccg ccatttctat gacgccatga
     8461 ccatccgcat gcccgactgg aaagcccggc gccaggccat ggcgaagtat ctgccttact
     8521 gtgtctgatg gtcagcggga atcgtcgccg ccagttccgg ccggccatct tggaaggtca
     8581 tgatgaagac gtctttaatg gcatcgtgat tggcatcgag ggaaatggtc cccgtcgcgc
     8641 catcgtaatc gatggtcttc accagttccc gggccacatc ggccgcttcg gctgactgac
     8701 agcgttcaat ggcatcggcc gtcatcatga aggcgtcgta ccccagggcg gcataactat
     8761 ccggccattc cccatacttt tcataataag cctgagaaaa gcgcttggcc gcatccgtcg
     8821 cgtcgtggcc ggcataatgg tccgtataat agacctgggt caaataatcc ttgtcgacat
     8881 cccgttccat cttggggcca ttccagccgt ccggccctaa gaggggcagg gaaacgccgt
     8941 gactgcgcag cagctcgata gccggtatcg tccagtcatc ataaccgggc agatagatga
     9001 cgtcgacctg ttcccgctgc aacaagggca ggtatttagt caggtccagt acattttcta
     9061 cgggaatata gacgggcacg tcaccgccgc tggcagcaaa ggcactgcgg aaatattcgg
     9121 ccaggccctg ggcatacatc ttatccggat aataaaagac tgccgcccgc ttgacatgga
     9181 gctgtttcag cgcatagtcg gccatggccc gcccctggga agcatcgata taagcggcgc
     9241 ggaaaatata gcggtacacc tcatgggact gggcatcgac ggtgatatcc ggctgtgtgc
     9301 aggccggact gatgagggga atcttcacgg cttccacgtt ggggatgaca gcccgcgtac
     9361 aatcggccaa gtccgggccg atgatggccg acacatggcg caccgtgagc tgctggatag
     9421 cggctacggc atcttccggt ttaccatgat tatcgaccga tacgagctgg acgggcttac
     9481 ctaagaggcc gccccggtca ttgaccatat ccttggccag ttcgatgccc cgttccgtcg
     9541 atgtcgaata ggacataccc ttgccgctca aggccaggtt cgtcccgatg aggacccctg
     9601 tattggctgg catcaaagaa cagccactca agagaaggat gacaaaaagc atacaaaagc
     9661 cgagtatctg catgtttctc ttcataaacg cgcctccttc ctgggggata gtagtaaaaa
     9721 aagggactgc cgcacgtgcg acagtcccct tttgagtttg tagtttttag tttacagcaa
     9781 atggtaaaaa attgaaaacc atcttctaca aactctaaac tttgtactac aaactttgaa
     9841 gacaaagaga ccgtgcatga tgacaaagac cggcatgcgc taatctctac ggcaggaaca
     9901 gcagcgggtt ggtcggggat ccattgacgc ggacttcgta atggacgtgc ggccctgtgc
     9961 tgcggccggt actgccggcc agggagatga tcgagccagc ccggacttcc tgtcctactg
    10021 tgactaaaag catggagtta tggccatagc gggtaacgaa gccattgcca tggtcgattt
    10081 cgacgaggtt gccatagccg tcgacccagc cggccatggt gactttaccg gctgccgtcg
    10141 cccggacagg cgtcccgtag tcgacgccga tgtcgatgcc ttcatggaag ccggaaccgc
    10201 cgccaatagg attgccgcga taaccgaagt tagacgtgac tgtcccgctg acgggccaga
    10261 tggaaggcgt cgtcgacgtg ctgctggcat cgccattatt tccgccgccc agggcaccac
    10321 cctgatattt atgcgtaatc tgctgcattt gccgaacctg ttcgcctgcc gtatcgctca
    10381 agaccgtctt caaggtgatg aagctgatga tgcgggcgtt actgcgggtt tccaagcggg
    10441 aggtggccaa taacaggtca ttgtagctgg catgagaaag gtccggagat ttcttttgtc
    10501 cccctgctac gccaccgtca ccttttgggc tgtcaccagc ccccgacccc ttgaccattt
    10561 gccgcagctc ctgatccagg gaatccattt tatccatctt ttcctggagc gatgtcgtct
    10621 tttcacgcat caattccagc tgctgttcat gcagtgcttc ctgggccttg gcctgctgaa
    10681 gcgaaacgaa ctcgtaaatg ccgatgcctg cagcgattac aatgacgacc actgcgacag
    10741 cacagccgcg gacgagtttc ttatattgcg ccggtgtcac gactaatggc tccgtccggt
    10801 ccgttttcag gaacgacggc caccatgccg gtgtttccca ggccttcatg cgttggcacc
    10861 atgattgcag gttgcgcatc catggatata tcttgatgtg gatcttcacg cttttcccct
    10921 ctttgttatc cttccagatc cttttgaaca gcctgcatgt caccgctgta attcaatgcg
    10981 ttgtccaggc tttccctttc ttcttcactc atgccataga gacacttgcc ctctacgatc
    11041 ggtttggcaa aaatacgcga ttcattgcgc ccatagacac tggaaagaca gaagccatca
    11101 tttttcccgt cgaggacggt aaaggcaaaa ctcagattgt tgccgatatt gtcaaaggca
    11161 ttatatttaa cgaacccgat tttacggaaa cagtgattct ggacagccat gatatccttc
    11221 tgcgtctggt ggatcatgtc gagtgtcccc tgcaggttat gcagcttatc gacatcgtca
    11281 cgcagcttcg cttcgacgct ggctcccgtt tcgtcttgca taaaaaaggt gtaattcgtt
    11341 tctaattttc tgattttctt gtgtaaaatg acgatataga cgagcaagag caataaaatc
    11401 aacccgccaa taccggcaag cgccatcgtg gcagcctgca tatcgacatt cagcatttct
    11461 ttcctcccat gaacatgaat atgcaaaaat attatatatg aaacgaagaa agaggtgacc
    11521 gcggcgtgtc ttctttttct tcagtcagta tcgacagcag ccgttcaaat tctgcttctg
    11581 acgaaaagga aatctcgatt ttcccctttt tccggccctt ccccagctgg atggccacat
    11641 cggtccccaa ggccatcttc agcttatcct gcagccaggc cacatgggcg tccggttccg
    11701 ggcggggctt ggcctgggtc atcagatggc ggaccagggc ttctgccttg cgggccgaca
    11761 gctcatgctt cataatgtac agagccgctt cccgctgcag tttcgcagac ggcagctgca
    11821 acagaggccg ggcctgtccc atggataggc ggcctgcagc gatccattcc ttgatgtcgg
    11881 ctggaagctg cagcagacgg accatgttgg ccacatggga ccggcttttc ccgactttct
    11941 gcgccgtctg ttcctgtgtc agtccaaagt ccttcatcag ccgttcatag gccatgcctt
    12001 cttcaatagc atccaggtcc tggcgctgca ggttctcgat gagagcaatt tctgccgctt
    12061 catcgggcgt atagcttcgt atgatagccg gcactgtttt caggccacag cgtttggccg
    12121 cccgcagccg ccgctcaccg gcaatgagct catatcggcc gggaccggcc ttctggacaa
    12181 cgacaggctg gacaagccca tgctgacgga tggaatccgc taaagcggcc agcccctgtt
    12241 catcaaaggt ctgccgcggc tgccggggat tggggacgat acattctacg gcgatttccg
    12301 ccggtgcgcc ccgttcagca ggagactcgt cgactgccac catgtccacc tcatttcttc
    12361 caaaattatt tttaaagtct atattatagt atatcgtcta tccaccattt aatcaaatat
    12421 ttattgccct gatttcattg atttttctaa caatttctat ggtataataa gaagcaaatg
    12481 tacagattca gaaaggatgt aaaaaatgcc aacatttacc gaccccttgc gggaaatcga
    12541 cgctgccagc gtcaaagaat acgccttgca gcgcggagct gctgaacttt ccgatgattt
    12601 cgtccaggaa gcgtgccagc gcgtcatgga actggccaaa ccccaggcta cgtgccagca
    12661 gtgcttctat gacagcacgt cccatatggt cctttgcgac agccccttta ccatcaacag
    12721 cccgactgtc caccagcgca tcgaagattc ggccatcgtc ctgatgtatg ccgttaccct
    12781 cggcgaagcc ctggaaaaag aagtcgatgc cctgttcatg aataaagaca tcacccgcgg
    12841 cctggctctc gattcggccc tggccgtcgc cacagcaggc tatacgaaac agctcatcga
    12901 catgctcgac gaagccggcg ccgataaagg ctataaagcc gcctggatca tgaatcccgg
    12961 cactggcgac tggccgtccg gccagatggc caacatcgcc caggccgtcc acgccgaaca
    13021 aatcggcgtc tccctcctgc ccagcggcat gctcatgcca cgcaaaaccg taaccggcgt
    13081 catcggcctg aacttcatcg gcggtggctg ctccggttcc tgcagctcct gcgccatggc
    13141 cggccattgc ggggaatagg gctaggggtt aggggctagg ggctaggggt tcgtggctcg
    13201 tggttcgtgg ctcgtagggg ccgtcccaaa gggcgtcccg ctacacgctg agggtttccc
    13261 gaagcgtcgt tgtcagcttg agtctgtcgc ggcggaacgc cgcatctgaa tccgaaaaaa
    13321 acgccggtcg taaaatcatg ccacatgaac catataatgt attaccgact ggtcacggcg
    13381 ggccgccttt caggacggcc cctacggatg ccccgattgg ggcgtttgtt tttgacgaac
    13441 cactaataac ttgaaaggga ctgtcgcaca aaggtttctg ccatcatgcg agattccctt
    13501 tgcttcatag tttgtagtat ggagtttgta gtttgtagaa gagggcttta aatttaaaac
    13561 ccttcactaa aaactacaaa ctgcgaactt aatactcaaa aagggcctgt cgcacgtaat
    13621 cgtgcacagg ctctttttat gtatgcaaaa ac
//
LOCUS       DAWCYS010000022        13583 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_303_length_13583_cov_8.663587, whole genome shotgun sequence.
ACCESSION   DAWCYS010000022 DAWCYS010000000
VERSION     DAWCYS010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_303_length_13583_cov_8.663587"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3042 a         3567 c         3687 g         3287 t
ORIGIN      
        1 gtgtaaactc gcttcgtcaa cttccgaggc ggcgtaaaag ttgtaaagtg ctgtttatta
       61 ttcatacagt ttgaaaacat cttccagcgc ttttttaata gccggatccg tgcagttcga
      121 cggctggcga gccggtccta cgtgatagcc gcgcatgttg acagctaatt taacgaccga
      181 attcggattg cccatctgca tgacattgcg gaaagctctg attttcttct gcgattcatt
      241 ggccttttcg atgttgcctt ctttccagta gttatagatg ctgaccatca attccgggaa
      301 gacattggag cagcccgaaa tggcaccggt accgccggcc atgagggccc agaggatgag
      361 ggagtcactg ccggccaata cgttaaggcg gggatctgta ttttcgatgt atttcagcat
      421 gttgtcgaag ttgccgctcg aatctttgat gccgatgatg ttttcatatt ttgccagggt
      481 cttgacggtt ttccagtcaa tggaattgcc tgtccgcgcc gggatgttgt acaacaaaat
      541 cggcatatcg acggcttcgg caatggcact gtaatgttct atcaagtcag cctgtgaagc
      601 ctttacaaaa tatggggtga tgatagataa gcaatcgaca ccgcccatct gctggactgc
      661 tttggacaat tcaattgttt ctttcgtcgt aacacagccc gtgccggcat agacggggac
      721 gcgaccctgg acggcttcga cggcagcttc gatgatttcc agtttttctt tagccgaaaa
      781 tgcatagaat tcgccgttcg tccccagcgg gaaaatccca tggacgccgg ctttgattaa
      841 gtggtcgata aaagcctggt attctttttt gttgaattct tccgtttccg tcaacgccgt
      901 aacaaccggt gtaatcaaac cttctgcttt aaacataatg tatctcgctc ctttgtgaat
      961 atatgtaaaa tttaatgttc tgcaaatcgt tttcctaaat cgacggcata gcacatgctg
     1021 tccgccttgg caatgccctt gccggcaata tcgaaggccg tgccatgatc gacggatgtc
     1081 cggatgatgg gcaggcccag cgtgacattg acgccggaat caaaggctac cattttcatg
     1141 gggatatgtc cctggtcatg gtacatagca acgacgatgt cgtagacgcc attataggct
     1201 ttcaagaaga ccgtatccgg cgggaccggc ccatcgacat cgaggccttc ctgctgggcc
     1261 gcctggacag ccgggatgat ttcatcaatt tcttcccggc caaagagccc ggcttcgccg
     1321 ctgtggggat tcaagccggc aacagcgatc cgcggcgttt cgacgcccaa ctgcttcatc
     1381 gccttatcgg ccagatgaac acattccagc accctggctt tcgtcgctct gtcacaggct
     1441 tcccgcaggg aacagtgagt cgatacgtgg acgacagaca tgtgttcgct ccaaagcatc
     1501 attgtataag atttcgtctt cgtcagggta gcgaagattt ccgtatgacc gtcgaagttg
     1561 tgaccgcctt tatggagggc ttccttattc aagggagccg tcgtgacaac gcggatatcg
     1621 cctttcaaag catcagaaat ggcccgggca atatattgat acgccgcatc accggcaacg
     1681 ggtgatacct gcccgatttc aatatctttg ccgatagcca ggtcgacgac agaaatgacg
     1741 ttgattttcc ctggctggaa gtcctgtacc ttttcaatgc gtaccagcgg cgtcgtcagc
     1801 tgaagccgtt gatgatagta ttgaagcacg tcgtagctgc cataaatcaa gacatattgt
     1861 tggtactccg ttttagcatc tatagcttta agggaaatct ccggcccgat accagccgga
     1921 tcgcccatgg taatgcctac aaaatttact gccactgtac ctcctccttt gtcacaatca
     1981 ttttttgttt gattgtgttc ttatctgttt gtttttgtta attagattat aggagagttt
     2041 ctctaataag tcaatggctt tttcgtatat acaccatgct attttttcat catatttcag
     2101 aggcaacttc caagaaaatc acagaaaaaa aagccagaga tggcatactc atgctcattc
     2161 ctggcttttt tcaattctct atatgtctga ttttataaca caaaacaaca ttttagaact
     2221 aaaaaaaatg taattaattg tatttcagtt cctggataca gcggaaaata tcttcttccg
     2281 taccaaaggc accagctttg gtgacgatag gcaatccttc ctggctgcct cccagcaaac
     2341 ggccttgcac aaaaccgggc actatctccc gttcaatatg ggacccgctg gcccctaact
     2401 gcaaaatgac ggcaatggcc gtatcgccac cggtcaaaaa caaaccgcta acggcggttt
     2461 cctgcaaaat agccggcacc atccggctta gggtctgttt ggtaaattcg gccaattcat
     2521 ccgtagaaat ccccttttcc cggccatagg cagcaaagtc ttcataatcc tggcggcagg
     2581 cagcccccgt gaggaccacg ctctggccag actgcaaaaa gtttacgatt ttctgaatcg
     2641 ctgtccttcc atcctgattt tcataaaggg ccctcatgtc cagtgccacg acgggaaggc
     2701 cccggttccg gcaatacgcc agctggtcca tggtcttgct gctgatgctg ccaataacgc
     2761 ccatggccgg gagctcccgc agctgtatct gtaatattcc ctgggccaaa ccggcagaac
     2821 cgatccacag gactttcttc cccgtctgac cggctttttc ggcaatccgt tccagctgcg
     2881 cctgctccat cgtatcaaag gaataagcta atccggtaaa gtcaagacga tcgtcctcaa
     2941 tggcatcgag ggtatagtga cagaccagct gctgcaggca cgtcgacaaa atccgttgta
     3001 cgtgatcttc ctgcaaggga ttccgcggat cacgggcgat ttccgtatcc agcaacggtg
     3061 tcccatggac gcaaagccgc cccgcttcga cagtccgccc ctgctcagga taagccggcg
     3121 caaagatgat gagttccggc gcataactat cgacaacagc ccggatttct tcttgcaaat
     3181 gaccgcgcag cgtcgaatcg actttcttat acagaaaacg gcagccaccg gtttgctgta
     3241 cctgctgtac ggccgcatac acgcggtcat aggcttcctg gccggaaacg acgcggctct
     3301 cgctgtcgat ggacaggctg ttgccattgg cttctatctg tgccgtatcc aggataacat
     3361 cgacaggaat cccgtgcttg caaagctgga cgcctgtatc attggctccc gtaaaatcat
     3421 cagcaatgat gacaaaatga tgcatgtgct ctccccctta aacgcggata aactgaacct
     3481 ggtgttcttc aaataaggac gcttcttctg cactggcctg atagtcactg acgaggacat
     3541 ccatatccca gaggtccagg atataataga gtttatgttt cttaaactta gaatggtctg
     3601 ccaggaggac gaccttatcc gatgcctgaa gacaagcccg tttgacatcg attttctcag
     3661 cattggccgt catgatgcgc caatccggcg tgatggcctg agtccctaaa aagcagatat
     3721 cgaaatgcat ggcccggacc atattggtcg ccaagacgcc gcacgtcgaa ctggattcga
     3781 cgtcaatatg gccgcctaag aggatgacat acagccccat gaccggagtc aggacctggg
     3841 caatggctaa atcattggtc acgacggtca gattatccca gcgctgtttg gccatcaatt
     3901 cagccagctc aaaattcgtc gtacccgcat cgagatagat gctcatagac gattcgataa
     3961 gaggcaaggc cgctgccgcc atgcgccgct tttcttcaat gtggactaat ttacgattgc
     4021 gatacgtcgc ttctttgaca gaagattcta ccaaaacagc gccgccgtgg cttttttcga
     4081 ctaagccgcc ttcttccaaa tgctgcaaat cccgccggat tgtcatcgtc gtgacatcaa
     4141 gctgctgggc caggtcattg accgttacag ctttgttctt gtacagttcg ttcaaaataa
     4201 actgccgtcg ttctgctggt aacatacact tcacctcatg tttatcctta catacttttc
     4261 tcatcatata atgtttgtat ttgttctttt gtgttaattc attataacaa gagaaaaaaa
     4321 aggcgtcaag gaaaaagggc ttgtcgcacg acgacaagcc ctttaaagtt gttagtggct
     4381 agttgttagt tgttagcgga tgggtttaaa tttaaaggtt ttctctgcaa actacaaact
     4441 ctgtgctaca aactataaag taaacagggc ctcgcatgct gtcagaaaca ttcatgcgac
     4501 agtcctattt aatggttcgt gattcgtaac tgcctgccat tataccagaa caggctgtgg
     4561 cgaaatcacc ctagcgggct cgccacgtgc gagcccctac gtgcggccgc tggccgtttt
     4621 ttgggcgacc tgcgacctgt gacctgcggc ctgcatttat ttttcatcgc ctgtatgcga
     4681 ttccgtaggg gacgcccaga tttttctggt ctgtcaattg caggatagtt ccctggacgg
     4741 acagtggctg cgggcggccc gctacgcgct gagggtttcc cgcagcgtcg ttgtcagttt
     4801 gagtctgtcg cggcgcagcc gcatctgagt ccgaggaaag gccttaaatt ttagaaatgg
     4861 aaatccagtt cagtccggaa gagattacgg tcatattcat ccgacgtact ccagtggcgt
     4921 ttctgtttgg aatacatcgt ttcccagata atgtttttcc acggggcata acggacaccg
     4981 gcaatccaac ccttggtgcc attccaaatg gagttccatt cgtcatcccc agcgagccag
     5041 ccgttttcgc catatttaat ataacgcaga taaaggccat aactgccagg ctttttataa
     5101 tcagcatctt tgtaattcaa acgcaaagcg atgctgttgt tctggaaatc ggcatcggtt
     5161 ttggcatagt cggcaataaa gcgcaagtct ttattgatgt tcgtagccat actgaggaca
     5221 tagccataac tgcgtttatt cgtcgtatcc tgattatcgt atactggcga tacgcggcca
     5281 taatttttat tgggagtccc atcgtcgtta aattccttca tgtccgtctg gtagtacgaa
     5341 acattgccag aaccgtactt ctcattttta cctaagctgc tctttgcata ggcaaagttg
     5401 atatccaggt tcttggccac agacccccaa acagcagcac cgtataagct ttccctgttc
     5461 cctgcacctg tcgaagcgaa ccagaaacca ctgccagtca atttcgttcc cggaatgggc
     5521 gctccgaatt ggataccgtc agtttcatta ccgacgagga cattctgaaa gcctaagccg
     5581 cgccaggcac gaccaatgct gacaaaacga ccgtctttct gcccttcgac ccaaattcgc
     5641 tgcatattgc cgtcatggcc ggaccaagtc tgttccccat tcgagttaaa ggcattttca
     5701 ctgcgccgat gaccgtcatt ataatggcgg ttcgtttcgc tttggaactt gaccgtccag
     5761 taatcgttga ccttaaagtc gccgcgcaag tccatgtaaa aacgattagt ctgtttatca
     5821 atagtgccgg aggtcgtatc tttgtcataa tcgtactgtg tacgcatgaa gccatacaat
     5881 ttgactcggt cgattttttc cttgttttcc tggatatcct tggtattctg ctcgatctgc
     5941 ttggtgttct tggcgacttg ctgctgcaag acgttgattt cgtcggcata ttcggcgtgc
     6001 agtttttcca ggaccgtctt gtccccgaag ttgccgccgc ctttttgcat ggctttggcg
     6061 acgatgctgg cgacttcgta gcgggtcatg gtgcggccgc cttcaaaggt gctgttgccc
     6121 atgccttcga tgacgccttc ttttgccagg taatccaggg cttcatagga ccagtggtct
     6181 ttggggacat cggagaagct gtcagccgat gcggccaggg ccgtgccggc agtgccgaga
     6241 gtcacggcag ccaggacaac cagggctttt ttattcaaat ccatggtaag gacctctttt
     6301 cttataaatt atcttccagc atggccccta cgctgtcata gactttacga gcctggtcct
     6361 ggatagccgg acgggccgtg gcgacggtgt agccgttata gcccaggatc atgggcaggt
     6421 cgttgatttc atcgccaata gccaagatgt ccgcaccgtc ccagccaaag agggagagcg
     6481 tgtgggcaat tgcctgttgt ttactgatat cggagggaat gatgtcgacg gcacagcggt
     6541 tgggataggc atgaacggta tcgccgaaat gggcgttgac ctgttcgctc gctgccaggg
     6601 catcgtcggg atcggtgaag cccagggaat actggtgaat cgtctggggc agatggtcga
     6661 tgtcatcttc cgttatcggc acgatgggat agtcccattc tttcgcttcc cgccagaccc
     6721 aggtgcccgg caaatcatgg cagagatagg tccggtcctg cgaagagaag gagaaatgga
     6781 aggacttgcg gaccagctcc agcttgctga cggccttgag gacggccggt tcgatgcggg
     6841 cttcccagag gacgcggtcc tggtcatcgc tgatgaggcc gccgttattg caggccaggt
     6901 aatcatattc gacgccatag tgtttgagct ggggcatgag catgccgtgg tcccgtccgg
     6961 acacgacgcc gaatttatgg cctgcagccc gccatctgcg gatggcttcg gcatcgcggg
     7021 ccgtaatgat atcgtcgcgg aacagggttc cgtcataatc gctggcagca atttttaaca
     7081 taataatcct cctcatacac gatagatgac ggcttcggcc gggcgcaatc ttccggcttc
     7141 ctgggcgtcg tagttgccca gaaggaccgt tgcgccttcc gtcagcgatg cgtcataatc
     7201 gatggtttcg ttggtaaaat tgaccagcgt caatgtcgcc tggtcgtcta agatacgctt
     7261 gaaagcgtaa atgcgttcat catcagccag cagctcttcg tagcgccctc gctgcaggat
     7321 ggctgccgct tccccctgga agcgttcggc ctggatgcgg cggtagtacc agaggaccga
     7381 atcagcatcg tgttcttcgc tctcagcaca gcactgcagg taatcgtcat ggacgggcag
     7441 ccacggcgtc cccgtcgtaa agccggcctg ggctgccgtc gtccactgca tgggcgtccg
     7501 ggcattatct cggctgtggg cctggacgag gtgcagggcc tctgccggtg caaagccttc
     7561 atccagggcc agctgatact ggccgtgcgt cgaaatgtca ttgtagtctt cgatacgggc
     7621 aaaggccgta ttggccatgc ctaattcctg gccttcgtaa atgaagggcg tcccgcgcag
     7681 gctgaacaag atggtcgcca tggctttggc cgccttcttc ttatccgtcc cttccgggaa
     7741 ataacggttg acacagcgta tctggtcgtg gttctcgaag aagatgggat accagccatt
     7801 ggctgccgtc gcctgctggc tgtcggacag ggccttcttg agcttggtca ggggccattt
     7861 ttcagcgcgg caccagactt catcgtcagg aaattccagg ttcacatggc tgaactcgaa
     7921 gagcatgctg aaagccccgt ccttaccgac ccactggggc aggtcagccg gcgtgacgcc
     7981 gttggcttcg gcgacggtga agatatcgtg gccgtcgaag accttccggc ggaattcgtg
     8041 gagaaagtcg aggatgcccg gcgtattagc gatcatcttg tggatggacg acaggccatc
     8101 ggctccgtcg acggggccgt ctgtaaagac ggccggtttc ttgatgtaga cgatggcatc
     8161 gatgcggaag ccgccgacgc ctttggctat ccagaaattg gccgcatcat agagggcctg
     8221 gcggacggcc ggattttccc agttcaaatc gggctgttcg acggcgaagg tgtgcaggta
     8281 atactgctgc cgttcttcgc accacgtcca ggccgggccg ccgaagatgg agcgccagtt
     8341 cgtcggcgcc gagccgtctt ctttggcatc gcgccagatg taccagtccg ctttgtcgtt
     8401 cgtgcggctg cgcttcgatt cctggaacca ggcgtgctgg tccgacgtgt ggttgtacac
     8461 caggtccatg acgacgcgga tatcccgctt cttcccttcg gcaatgaggg tatccatatc
     8521 ttctatcatg ccgtagcgcc cgtcgatggc cgtataatcg ctgatatcgt agccgttgtc
     8581 gaccatgggc gacgggtaga ccggcgtcag ccagatggcc ccgacaccga gcttcttgag
     8641 ataatccaag tggcgcgtaa cgcctgcaat atcgccttcg ccgctgcccg tcgtgtcctg
     8701 gaagctgcgg ggataaatct gatagacagc cgtctgctgc caccatttca aagtatccat
     8761 aaaaccctcc tagaaaatga caatcggtgt ttcaccagcg acgacaggct ggtccggttc
     8821 cttcttcatc tgcgggtatt ccgtcgagtt ggtgacggcc atgatgacga tgtcgttata
     8881 gccggctttg gcgatggcat cgcggtcgaa gcggatcaag gggtcaccgg ctttgacctg
     8941 ggcgtcctgc tgggtcagca gcgtgaaccc gtcgccattc atattgactg tatccaagcc
     9001 gatgtggatg agcacttcgg caccgttgct cagttccagg ccaatcgcat ggagcgagct
     9061 ttccatgatc atggaaacgg tcgcgtctgc cggggcgacg atggtatcgc ccgacggttt
     9121 gatagcgacg ccatcgccca tcatcttctg cgagaaggca tcgtccggta cttcgctgat
     9181 gtcgacgagg tcgccggaga caaaggcttt cagcttcatg gaacggtcgt cttcatcgac
     9241 ggaagcgatg gattcatcag ccggagctgc cgtcgtttct gacgaggctt ctttcgtttc
     9301 cggaaccgtg ttttctacgg ctgccggcat ttcccctttc atgagggcgt cgaagtggcc
     9361 gcggacattg ggcacggaca ggccgacgat gacctggatg gcgtggccgt tcttgaccag
     9421 gccaaaggca ccggcattgg tgaacttgct ggtcgcagcg accttatccg ggtcgttgac
     9481 cgtgacgcgc aggcgggttg cgcagttggt gacttcgcgg atgttatccg gaccgccgag
     9541 gcaatcgagg aagacctggg ctttcagggc atcggcaccg ccggcaatgc ctttcttttc
     9601 catttctttc ttggctttgt attccgcttt ggtgaacaat ttgtcttcgc cgccatcatc
     9661 ggtacggccc ggcgtgggca aatcgaagtg aaggatgagg aagcggaaga cgacgaagta
     9721 aatggccgta aagccgaggc caatggcaat ctgcaaaatg taggtccagg catgatagcg
     9781 gaacaagggg atccagttct ggacgaagca gtcgatgagg ccgccgccga agttgcccga
     9841 caggccgatg aaatagagcg tcgccgacaa ggtagccgct aagacggcat ggacgacgta
     9901 caagagcgga gcgacgaaca agaaggtaaa ttcaatcggt tcggtaatgc cgcagaggac
     9961 ggctgtaatc gtagccggaa tgagcagggt tgcgactttc ttgcgttttt ccggtttggc
    10021 acaatagtac atggccaggg ctgcgccggg caggccgaag actttggcac tgccgtggag
    10081 ggcaaagccg ccttggggga acatgtcccg caaggaatga gcactggtgg cgaaatcatt
    10141 gaggtgctgc agccagtaag cctggatgcc gccgtcgcag acagccggac cgtaaatgaa
    10201 gggcaggtaa ataaaatgat gcaggcctgc cggcaagaga atgcgttccg agaaggtgta
    10261 agcccagacg ccgaagatgc cgctggtctt gaggaagaac tggaactgtt cgataccgtg
    10321 ctggataggc ggccagatgt aacagaagag gagcgccatg ggcagcatga ccaggaagcc
    10381 gacggcgacg acgaaggccg gtcccttgaa gatgccgagc cagtccggga tttccgtatc
    10441 atagaaatgg ttgtgcaggt aggccgagat gcaggcaatg aagatagcgc cgagcatgcc
    10501 catgtccagg gtcttgatgt tggcaatcat ggccaggccc gtgccggcac cggcgttctg
    10561 actatagtcg acgccgaagt agctgccgtg aagggtcagg ataccggcga tgaaatagtt
    10621 aaataccatg tagatgacga aaccttccat ggcacagcgg gcctgttctt tcttggcaaa
    10681 gccaatggga acggcgatgg caaagaggat cggcatctga ttgaagacgg tccaggcgcc
    10741 ctgctggacg acgtaccaga aatcatacca gagggtccct ttttctgcca ggctgccgaa
    10801 gaggcccgta ttgctgaaga tgatcgacaa ggcgacagtc agcccgaaga aggcgaataa
    10861 gatgaccggt gtgtacatag cgccgccaaa acgctgcagt tcctgcatca cgcggtcctt
    10921 gttgaatgac atagacaatt cctcccgttt gtgaaacaaa gtacataagc tttccttagt
    10981 aacaggatat aaaaaaaacg ccgcgaatac aatagattcg cggcgttctg gcacacggcc
    11041 cccggcctgt aacctggtac atttcctgta ctttggtaca gcctatcggg aagcctcttt
    11101 ttcttgtttc aaatggtgca gatacataca atatttcaca taccatatct cagccaggac
    11161 aaaataaggc atcatggact gaaaggcgac gtggtcgtct tccttgtcat agagctggaa
    11221 gcgggccggc gagacgtaaa aattgaccgt cgacagcgac gccagggtat tgtcgtgcag
    11281 gcgggtaatg gaaatgaagg gaatgtcccg cagtttcagg gcctgggcca gttccttaac
    11341 ggccggcgat tcgccggaga aggagacgat aatgaacaag tcgtctttgg tcatggtccg
    11401 gatgaggttg cggaactctt cccgtgccgg tacgtcgtag agcatgacgt tatcgtagaa
    11461 aaaaagacgc ttcagctcct ggacgacgtt attctggacg tagcccgacg cataggtgaa
    11521 aatccggttg gcttcatgga cgagggcgct ggcgctatcg aagttacggc tgcacagctt
    11581 gtccacggtc tgttcataga agcgctggag ttcgtccatg acattcgtat catagcagga
    11641 aatttccggt tcttcccgct tgaggagcat cttgaattca ccgaagccgt cgaggcctaa
    11701 tttctgggca aagcggacaa tcgtcgtcgt cgagacgtga caggccttgg ccagttcatg
    11761 gatcgagctg tggcggacag cggcgcggtg ctgcaagatg tacttccata tccccatatc
    11821 cgtattattg agctgctgac gatgggcgtt gatgagttcg gcaagttgca tggctggttc
    11881 ccccttcacg aaacaggccc cggcggaaaa atccgcaggg gccgggtcat agtttcatta
    11941 taaggaatcc agggacggac gtcaagctgg tgggcgctgt gccgcacaga aaaaggctgc
    12001 cattttacta caaactacaa actcaaaact ccataagaag ctgtctgtat agaatagcac
    12061 ttccgctggc tgactattat ctacaaagtg ccgcagcgat ggcagcgccg acggaggcgc
    12121 cgttgctgtt ggggatgacg tggatgcgcg tattttcggt cggagccatg cagaagacgc
    12181 agatggcatc ttcgatgagc gacggaacgc ctttgatctt ttcgaccagg ctgccttcga
    12241 tggcgatgga ctgttccgta aagcccttat cctctgccaa gtgactgaga aggccgaagc
    12301 tggccgcccc gatgagctgg gctgcccggg caaagacggc ccggaccagg gcacagaggc
    12361 cttcttgctc ttcttcgctg acggcgaaac cgagcagttc ttctgccgtc tgctggccgg
    12421 aaatgagttc gttcatgtcg cgcgtcgtaa aatacgggat gtcccgtttg aggtattcgc
    12481 caaagacgac gcggcagact tcgctcaaat agcggccgcc gatcatcttt tcaaagggat
    12541 ggtcgccagg cagctgagac gcttcatcga cgaggcggtc ccagaccgta gccgggccgc
    12601 cgtcgaagcc ggccgcttcc aggttgacga tcatctgcag ggccggttca taatagcaca
    12661 tgttaaagcc cgtgccacag acgatgccga tgcgcgacgg gttgtgctga tagaccgaag
    12721 aaaggagggt cgccgtcgta tcgttgagca gggccaccgg ttcgatgcgg tccaggccgg
    12781 cccggacaag ggcctctttc agcaggccat tgatggactg gccggccacg ccgggaacgg
    12841 cgatttcctt ggaccaggcg atgaggctgg catccttggc cgacgtctgg tcgacggcaa
    12901 aggaaaaggt aaagcccagt ttatagtcgt gattgccgtc agccgcttcc ttgacgatgg
    12961 cggcgatgaa gtcgaagagt tcgtctgccg tcttcgacga agccgtgtag tcgtagtcag
    13021 cgcatttcaa gggtttggcc accttgcttt ctacggcaaa gccctgttcg cccatgaggc
    13081 ggatgcgcga agcccggacg ttcgtgccgc cgaaatccag ggccaggaag gtccccgttt
    13141 cccgtcccgt cggcaagccc agataggtgc gcagcgccga aagggagctc tccttccctt
    13201 ccagagcgtc ttcggcactc ttccggaagt gctgtgccag ttcccgcagc tctgcgccgg
    13261 ataactgcaa tgcctgtctc attttttccc aattttttat cgtatctgcc atgatcgttg
    13321 gcctcctgtc tgtacgcaaa aacatctata ttctcatgat aatgaaaaaa ggccataaag
    13381 acaactcttt tatggccttt cggttccgta acacgacaat ggacggtagt acaaaatatg
    13441 gatattggta caggcgctgc gcgtaaaaat aaaaaggaac cgtgcacgat ggtgaagacc
    13501 ttcatgctcc agttccttga atagagtttg gtgggcgata acaggatcga actgctgaca
    13561 tcctgcttgt aaggcaggcg ctc
//
LOCUS       DAWCYS010000023        13171 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_314_length_13171_cov_6.552760, whole genome shotgun sequence.
ACCESSION   DAWCYS010000023 DAWCYS010000000
VERSION     DAWCYS010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13171)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_13171_cov_6.552760"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2678 a         3539 c         3832 g         3122 t
ORIGIN      
        1 ggggacgatg acgttcgtcg aatcgaggag gccgcccaag cccacttcga tgacggcgta
       61 atcgacgccg atatagtcga agtaccagaa agcagcagcc gtaatcattt caaattccgt
      121 cggcccttcc ccgccggcag cgaccatggc cgtcacggcg tctttgacga cggatacggc
      181 tttggcaaag tcgtcgcggg aaatgtcatg gttgtcgata tggatccgtt ccgtatagtc
      241 ttccagatga ggcgacgtgt agcggcccgt ccggaagccc gcttcagtca ggaccgaggc
      301 gatcatagcc gtcaccgagc ccttgccgtt ggtcccggtg acgtggatgg tcttatagtt
      361 gttctgggga ttgtccagcc gttccagtaa ggcctggata cggccgagcc ccagctggat
      421 gccaaagaca ttggctgatt ccagatagtc gattgcttgt tcgtaggtca tagctgcctc
      481 ttagagttta gccaggtctt ccatgcgctt ggtcaaagca gccatctttt cctggtagtc
      541 ggcgagcttg cccttttctt tttcgacgac cgctgccggt gctttcgaga ggaagccctg
      601 gttggacagc ttcttgtcga ggcgggcgat ttccttctgg agtttttcct gttccttctg
      661 gacgcgggcc gtttcttttt cgacgtcgat gaggtctttg agctgtaagt agacttcaat
      721 gcccgggaca acggcgacga cggcattttc cggtttggca tcgcccgcac cgaggagggt
      781 tactttatcg gcaaaggcca gggtatggaa gtaatcttcg taagtcttca aggtctgagc
      841 catgtcttcc gtagccggag ccaggatgac cggcgccttc ttgcccatgg ggacgttcga
      901 ttcggcgcgg aggttacgga tacccttgat ggcttccatc atggtattca tcgtagcggc
      961 atagacccgg aagttcaatt tatcctgcgt cttcggccag ggagcgacga tgatgctttc
     1021 gccttcgtga ggcaggtgct gccagaggtg ttccgtaacg aagggcatga agggatggag
     1081 catttccagg gtgtgcgtca ggacatagac caggacgtac tgaaccgtgt gcttgtcccg
     1141 ttcgctttcg gcctggtaaa ggcgtttctt ggccagttcg atgtaccagt cgcagtagga
     1201 gttccagatg aagttgtaaa cggcatcggc agcgtcgccg agttcaaatt tatccagatc
     1261 ttccgtgact ttggcgacgg tttcattgaa ggatgtgagg atccagcggt cggccagggt
     1321 caggtcttta tcttcaggga tgaaggtttc gtcgaaatca ccgaggttca tgatgacgaa
     1381 tttagaagcg ttccagatct tattggcaaa gttacggttc gcttcgacgc gttccatgta
     1441 gaagcgcatg tcgttgcccg gcgtattgcc cgtgaccagg gtgaaacgca gggcatcggc
     1501 gccgtactgt tcgcagacgc cgaggggatc gatgccgttg cccagggatt tactcatctt
     1561 gcggccctgg ctgtcgcgga ccaggccgtg gatgaagacg ttcttgaagg ggatttcctt
     1621 catgaattcg caggtcatga acatcatgcg ggcgacccag aagaaaatga tgtcatagcc
     1681 cgtgaccagg acgctggtcg ggaagaactg ctggatatcc ggcgtcttat cgggccagcc
     1741 catagtcgag aacggccaga gagccgaact gaaccatgta tcgaggacgt ccgggtcgcg
     1801 atggatatgt ttgctgtggc agtgctgaca ttctgtgatg tcttcccggc tgacggaaga
     1861 cttgccgcag tcatcgcagt accagacagg gatctgatgg ccccaccaga gctgacggct
     1921 gatgcaccag tcgtggacgt cttccagcca gtgattatag gtcttgacga aacggtcggg
     1981 gatgaacttc gtcttgccgt cttcgacggc cttgagggcg gcttcagtca agggtttcat
     2041 cttgacgaac cactgcttgg tgacgaacgg ttcgactgtc gtcttgcagc gcgagcaatg
     2101 gccgacggca tgggtcagtt cttcgatgtg gtccaggagg cccaattctt tcaagtcggc
     2161 gacgacggct ttccggcagt cttcccgggt catgccgttg tatttggcac cggctttttc
     2221 attcatcgtg ccgtcgaggt tcatgatcat gatggattcc aggttgtggc gctggcccat
     2281 ttcaaagtcg ttcgggtcgt gagccggcgt aatcttgacg gcgcccgtac cgaagccgat
     2341 atcgacgtaa tcgtcggcga tgacttcgac ttcgcggtcg atgagaggaa cgacgacttt
     2401 cttgccgatg tatttcttca tgcggtcgtc agccgggttg acggcgacag ccgtatcgcc
     2461 catgatcgtt tccggacggg tcgtagcgat catgatgtag tcatcttcgc cgacgacagg
     2521 gtatttgatg taccagagat ggccctggac gtcggaatgg tcgacttcga tatccgacag
     2581 ggccgtatgg cagtgcgggc accagttggt gatgcgctgg ccctggtaga tgaggccttt
     2641 ttcatagagc gttacgaaga cttcgcggac ggcgcgggcg caggtatcgt ccatggtgaa
     2701 gcgcttgcgg ctccagtcgc aggaagatcc gaggcggcgg acctgttttt cgatgcggct
     2761 gccgtactgt tccttccagg cccagacgcg tttcaggaat tcttcacggc cgatgtcgta
     2821 gcgggacttg ccttcttctt tggccagctg ctgttcgact ttgacctggg ttgcaatgcc
     2881 agcgtggtcc gtgccgggga tccaggcgac gttatcgcct tccatgcgtt tgaagcggac
     2941 gaggatatcc tgcagggtat tgtccagggc gtggcccatg tggagctgac ccgtgacgtt
     3001 cggcggcggc atgacgatgc tgaacggttc tttgctcgga tccgggtcct gatggaagta
     3061 gccattcttt tcccaaaaat cataccagga catttcaatg tcgttcggtg cataagcgcc
     3121 taactgttcc ttattccatt tcatctatat tccctccaaa acaaaaaaga gcattccctc
     3181 gcctaagggt attccttagg acgaaagaat gctgtatctt ccgcggtacc acctaatttg
     3241 ccgcttgcgc gaccgcttca tggcacaggt aacgtctgct gcacgggcag gcctttcctc
     3301 ctgccagctc ccgggtgacg cgccctaccc tctttggatg ccctcgcacc ggactcggca
     3361 tctcgctgac aaaaagaaat aaggcttctt ccgctcatcg cttttatgtg ataaatattg
     3421 tagcaagttt acttgggcct gtcaaggtac tccgcataaa atcaatagac gccctgcttg
     3481 aagagggtca tcatttccat ggtcaggctg gccttttccg tatcttccgg aatgtccttg
     3541 cggtcgatcc agccggccgt ggccaattcg ccgtggtcga ctttcagcgt atcgtcgccg
     3601 tcgaggtcgc agaagaagcc aaagagcagc gtatccgtga agcaccaggg ctggctctta
     3661 tagaactgca gattcttgac acggatgccc acttcttcca tgactacccg gtggaccgtt
     3721 tcttcgatgg tctcaccgac ttcggcaaaa ccggccagca aggcgatatt cttgtattcc
     3781 cgtccggcat acttcgagac cagcagtttg tcgccatggc ggacggcgac gatgacggcc
     3841 ggacagatgc gcggataaat catggtgtgg cagtggtcgc agtgtaccat gcgttcgttc
     3901 tcgctgtgta ccatggggcg gccgcaatgg ccacaataga tgtggttgct gtaccagttg
     3961 tgcagggaca ggccggcgat gccggcaaag gccaggtaac gcggccgggc agcgcgcatg
     4021 aaattgatgg acacataggt aaagccgtcc cgttccggca gggcttcctt cgccaggtag
     4081 aactgggccc ggtcttcggc aaagagccag cggaactggt cttctccgat gtcggcatag
     4141 tcggcatggc ggggatagac gaattcttta tcgcccgtca gccggaccag gaccttgcgg
     4201 ccctgataag acagcagaat accatcccgc gccagaggca cagggtggta ttcgacatgg
     4261 tacgtgtgat tgccaatgtc ttgtatcatt cgacgagttt cgctccttca ttgctgcatt
     4321 tcaggatatg gcccgtagcg gcgatgccgt ggtcgttgaa gcagttgatc atggtgtcca
     4381 tgatctgcac ttcctgatcc gtcgcaaagg cgatgagcgt cgaaccggaa ccgctgatgg
     4441 tagcgcccag ggcaccggct tcgacggcct tcttgaggac cagttcgccg ctggggatga
     4501 ggcgcaggcg gtagggcacg tgcagtttat cctgcaggcc ataggcgatg cggtcgtact
     4561 gcttggtgaa cagcgaagaa acgaggaagc tgacgcggct gacgttgaag acggcgctct
     4621 ggtagtcaat ggtcttgggt agggccttgc gggcttcttc cgtcgaaact tcgacttccg
     4681 ggctgaccgt aatgaaagac aggtcatcgt cgatggggat ggaattggtc agggtcttgc
     4741 catcgaccat gatagaaacg gacaggccgc cgtaaatggc aggagccacg ttgtcaggat
     4801 ggccttccag gacggtcgcc atggtcagca tatcttcgcg ggtcatctta ttgcccgtca
     4861 aggcattgcc gatgaggatg ccgccgacga gggctgccga actgcttccc aggccgcggg
     4921 ccggcggaat agcattgacc agccgcagtg tgccgccctt gggcagggac tggcggttct
     4981 tgatggccac ggccttcatg gcctgtccga ccatattctc atcgaaatta cggggcaggg
     5041 aattggctcc caaaccttcg atttcgacag tgatcgtgtc tttatccggt tcttccgtaa
     5101 aataaatgtc attgtacaaa gtcagcgcaa tcccgacggc atcaaagccg gagccgacgt
     5161 tagccgacgt ggccggaatc tgtgcgtgca gcgtttttgt catttcttac acctcgatta
     5221 cacgaatggc attcgcaatc ttgcgaacgc acgacaagtc ttcgattttc tggagcgaag
     5281 cctggaccag gcctgctggc gatttgtcga tgaggatgac gatttcagcg atttctgccg
     5341 tttcatcctt ctggatgagg gagcggatac tgatattgtt ttctgccagt accttggaaa
     5401 cagccgccag gacgcccggc gcattgttga cgatcatgcg gatgtagtac ggcgaggaaa
     5461 tgtcggcctg cggtttgagc ttggcttcgt gccatacgta atcccgtttg cccgtcatct
     5521 tcaggttgcg gtggaggatg acgttcatga cgtcgctgac aacggcactg gctgtcggca
     5581 aggagccggc gccctggccg tagaacatga cgtcgccgac gcagttgccg cggacgcaga
     5641 cggcattgta agcgccgtcg acattggcca gcgggtggcc tttgcggatg aaggccggat
     5701 ggacgttgag ggaaatgccg tcttcatcct ggcgtgcaat ggacaggagc ttgatgacat
     5761 agcccatttc cgtggcgtgt tcaatatctt ctttgctaat gttttcaatg ccttcgacat
     5821 ggacgtcgtt gaaggtcaca gccgagtgga agcccagcgt tgccaggatg gcgatcttgc
     5881 gggccgcatc gtaaccgctg acgtcgttcg tcgggtccgc ttcggcatag cccttttcct
     5941 gggcttcctt gaggacgtct tcatacgacg agccataggc ggtcatgttg gacaagatga
     6001 agttcgtcgt gccgttcatg atgccgatga tttcttcgat gcggttgctg ttgagcgacg
     6061 aatacatcgg gcgcacgatg gggatgccgc cggcgacact ggcttcgaac tggaagtcca
     6121 cgttgtgctg ctgcgataat ttcaatagcg gaatgccgta atcggcgatg aggtccttat
     6181 tggcactgat gacgctcttg ccgcgcagga agcactgttc gatgcagtcc ttggcaaaat
     6241 cggcgctgcc catgacttcg acgacaatgt cgatttcatc gtcgttgatg atatcgttga
     6301 aatccgtcgt gagctggatg gaatcaggta agtcttccat cttttcgtac ttattcaaat
     6361 cgcggatgag gacccgcttg atgttgattt tcgtattgag ctgttcttca atgagcggac
     6421 cgttcatggc cagtaagtcg acaacccctt ttccgaccgt accgcagcct aacaaggcaa
     6481 tggaaacatt tttcatgagc ctactccccc ttttttatct tacgcttgtc cgatgatatc
     6541 gactttgctg acaccggaca gcttttccaa cgttttaatc agttcatcga caggaacggt
     6601 catatccttg gtttcgatgg acaagctgac atcagccatt ccctgcaagg gaatcccctg
     6661 ggtgatggtc atgatactgc cgttattggc agcgaccgtg ttgagcactt gcgataattc
     6721 cccaggccgg tcgaagatca tgaccgacaa ggtgacgatt ttcccctgag tcacatcgta
     6781 aaacgggaag acatagtcct tatacttata gtacgcactg cggctcaagt ccatcatgcg
     6841 gacggcttcg ttgatggtgc gcgtctgatg atgcttgaga atttccttga ccttgatggt
     6901 cttcttgatg gcttcgggca gaatgtcttc gcggaccagg taaaatttat ggttgctgtc
     6961 ttctttcact cggcttcctc cctttcgaga aaatattgat acgtcgccat gaggcccggc
     7021 atgagtttca tcttcggccg ccagcggagc gtttcgatgg ccttgccatt ggccagggac
     7081 gaccggaaaa tatcgccctt gcgggcttct ccataagaaa cggccacacc ggcatgagag
     7141 aaatacaaga gaatctcttt cagggcgttg atggtcgtct cgatctgggt gctgatgtta
     7201 taaatccctg acggcacgcc gctgtccata gcggcgatat tggcgcgggc cacatccttg
     7261 acgtagacga agtcgcggga ctgttcaccg tcgccgaaaa tcgtcaggtc ctgaccgcag
     7321 gccaatgctc tggcaaagag gccgacgaca ccgccttcgc cgccattgcc ctggcgttcg
     7381 ccatatacat tggcatagcg caggatggca tagggcaggc cgaacaggct gccatagagc
     7441 tggatatact tttctgcagc cactttggtc aagccgtaga acgacgccgg tgccagtggt
     7501 tccgattcgc tcaggggcac tttaggatta tcgccataga cggcggccga cgacgaatag
     7561 atgaatttct gtacaccctg acggcggcac tgttccagga cattaatcag gccgatgacg
     7621 ttgagttcgg catcttccct gggatgggcc agggagtagg atacctgggt ctgggccgct
     7681 tcatgaaaga cgacgtcgaa atggccttct tcaaagaggg cctgcagggc tgcttcgtcg
     7741 cggatatcgc cctcgacgaa acgggcctct gtcggaatct ggtcccggca gcctgtactc
     7801 agattatcaa agacggtcac gtcatggccg tcttccagca aggcgtcgac gaggtgggaa
     7861 ccgatgaagc cggccccgcc ggtaactaaa atatgcaaag taagacctcc aatccttacg
     7921 gcaggtcacc gtaagtaatg agttcaatct gtttggcttc cagctgctgg cggatagccg
     7981 gtgacagcag ggcctggaat tcatcctgcc agtgatagcc ccaggcaaag gactggctga
     8041 ggacagccgt actgcggcct ggatgcatca tgatttcatg ggtcccaaaa ggcagtctgt
     8101 cgatgagata gtgcagattg ctttccgtca tgtgcccgcc gtcgaccatg ccccagaaaa
     8161 agtccgtcgt cgtaaagtgc aggcgccgga ccgaggccat ggctttcttc gacaagtagg
     8221 tcaggccgtc gcggccggca atgcgcacgg gattggccga caatacctta taggaatagg
     8281 cttccctggg gatgcgcagg cggtggatac cgtacttcat catcagcgac tggacgatgg
     8341 accagacctg cggcaggacg tgcaggtgct ggtggctgtc caggtggtcg atgggaaggc
     8401 ccgtagccat gattttttcc atctgggcgt cgagttcccg gtagacctga ccgtaatcga
     8461 gttttccctg acaggcccgc ttgatgactt ccgtatagct ttccggcagc aggccgtcgg
     8521 gctggaccag ggtcggcact tccctggccg acaggaccgt cggcagagaa ccgaccaggc
     8581 tgagatggat gccgatgccc aggccgggac atttccgggc caggtcgacg gcctcgtcaa
     8641 aggccgggcc gttggccagc agagacgtgc tggtcaggac accttggtcg caggcttcga
     8701 tgacggcctg attgacttct gtatggatgc cgaaatcatc ggcattgatg atgactctgt
     8761 tcatctttca aactccccgt cctttcagga cgtactacat aatgagctta atgactatta
     8821 tacatggaca atcgtatttt gacaatatcc tctgcccatt ctattccgca aattgccgat
     8881 atgccgcttc cttcactttg cgctgccaat ccttcagggg cgtccctgtc tcagcggcgg
     8941 cagcccggca atcatcgtat tctgccgata tgttgacgac cttgccgtca tagcggcaga
     9001 ccttgcagtg caggtcgcgg ccatcgacgc ggaccgtcgt gatgagccgg tcgcaggcca
     9061 ggcgggtcac tgggaagaag cggacgccaa tggacgtcgt ctccctgaca atgacgccga
     9121 tgagggtgtc gatgagttcc tgacggcaca agacgcagag catagaggcc atgcggcctt
     9181 tcttcatcat catcggcatg gcccagacat cgttggcgcc ctgagccagg atgagggaaa
     9241 agacgtattc catgttctgc gggttcatgt catcgatatt ggcttccagc tgccataatg
     9301 tttccatttc catcacctca agcgattcat cgtatcggcc aggcggccag cgccgaagcc
     9361 gttgtcgata ttgacgacgg atacgccggc agcacagctg ttgagcatcg ccaggagggc
     9421 cgacaggccg tggaaattgg caccatatcc gatactggtc ggcacagcga tgaccggttt
     9481 gtcgacgagg ccgccgacga cgctggccag agccccttcc atgccggcga cgacgatatt
     9541 tacgttggcc cggcggatat catcgacgcg ggccaggagg cggtggatgc cggcgacgcc
     9601 gacgtcgtag atgcgttcaa cgcggttgcc catgatttcc gccgtcaagg ccgcttcttc
     9661 ggcaatgggg atatcgctcg tcccggcggt aatgaccgaa atgaggcggt cgccattgac
     9721 gacggtttta tccttatgct gatagaccat acgggccaat tcgtcatact gggcagccgg
     9781 atggcgtccg gccagggcgt cgtagacggc cttatcggcc cgcgtgccga tgacgttgtc
     9841 gtacttctcc ataaggatgt ccaggatccg taccgactga ttgacagtct tgccggcaca
     9901 gtagacgact tccgggaagc cgcagcgctg ctgacgctgc aagtccagtt tggcaaagcc
     9961 caaatcgagg gtttccttat tttcttccat agttactcct taaaaccatt tttttcccat
    10021 gccttatata agacgatgcc gaccgacatg gacaggttca gcgattccgt atcgggacgc
    10081 atggggatgg tcagtacgtc atcgcagcag tccaggaaag gaccaggcag gccgttgccc
    10141 tcgttgccga aggccaggat gacaggctgg ctgtagtcgg cgtcgacata cgagacggcc
    10201 ccttccggag ccgtcccata gacctggcgg tcctcgtcgg cacagaactg gtccagctgt
    10261 tcatcggtca cgtcctggac gacggggacc ttgaagatag cccccatggc actgcggatg
    10321 gtcttgtcat tatagacgtc gacactgcct tcgctgagca ggacggcgcc ggcattggcc
    10381 gcagccgccg tgcgcaggat ggtccccaga ttgcccgggt cctggatgga gcgcagatag
    10441 aggacggcct tttccggcgc aatggccggc aggctgtcga aagtataggc aaagaaaggc
    10501 agcatggccg ccaatccctg gggcgtcctg gtgtctttca gcttgtcata gacgctgtcg
    10561 gtgacggcat aaaaagtcca tcccaattcg aggcctctgg catagagggc ttcaaagcca
    10621 tccagggccc gccccttttc cgagacgaag cagatggcat cgggaatagc catggccgct
    10681 gcgtcggcaa tgacccgcag gccttcgacg aagacgcgct gatgggcctg gcggcctttc
    10741 ttctgcttca gggagcaggc catcttgacc cactggttgt cgcgggacgt aatttcgttc
    10801 atagtacctc ctatctaccc gccatgcgga taaaaaaagg gacctcgcat gataactttc
    10861 atgcgacagt ccctctcgta taagccatga ttactgctga tccggcttgt cgtcctgatg
    10921 tccgttattc ttgagcaggc tttcgcggtt ctgctggatg ctggacaagg tcttttccaa
    10981 tgtcgattca acgtatttga ggacatcgcc ggcataggta atggagctgt tcttcagctg
    11041 ttccgacgaa tcgttggcat tctggatgat ctggttggca tgttcctgag cctgccgtac
    11101 cagttcgctt tcttctgtca atttggcaat ataatccttg gcctgggcga ccatgctttc
    11161 ggcatgatgc tgggcatcgg aaatgatctt atctttttcg gacaagactt tcttggcctg
    11221 ttccaattcg gccggcatgg attctttcaa atcgtcgatg atctgaagga tttcttcttc
    11281 ttcgaccatc ttcttattgg taaaaggcat cttcgaggcg ttcattacca aactttccaa
    11341 atcttctaac agtttatccg aattcatgta ttctccactc ctaagcattg tacttgtctg
    11401 caaacttctc tttcagtttc tgttcgacat aatcgggaac catatcgtcg agcttgccgc
    11461 cgaagcaggc cagttcgcgg atacctgtcg agctgatata cgaatactgg gtactggtca
    11521 tgatgaagac cgtttcgata cgcggttcca ccttcttcat gagcagggcc cgctggaatt
    11581 cgtattcaaa atcgctcagg gcgcgcaggc cgcggatgat gatagtcgca ccgtgttcga
    11641 tggcatattc gttgagcagg ccatggaagc tgacgacttc gatgttggga atgtgttttg
    11701 tcgtttcccg caacatctct tcccgttctg ccatcgagaa caagggcttc tttgacgggt
    11761 tctcaaagac agcgacgatg agcttgtcga caagctggct cccccgttca aaaatgtcaa
    11821 tgtgtccgtt ggtgacagga tcaaaactgc cgggacaaat accgattcgc actttctaga
    11881 ctccttcctg acgacggcgg ataaaggcaa tccgcgtgtc tttcccatag gatttttctc
    11941 tgatgacctc atacaatggg ctctccgtta taccttcgtt ccggctgtat tccatcagaa
    12001 tcaatcctcc cggagccagc aaatcgtatt cacctatcaa agcgattgta tcgttcacca
    12061 aatttttgtt atacggcggg tccgcaaaga tataatcaaa tttgcgtccc tgcagccgtg
    12121 ccagggcttt ggccgagtta aggcgcagca cttcgacgca gtctgcaaag ccgcaaaggg
    12181 ctgcattctg gcggatgagc tgctgcgtgt aatggtcgat gaagacagcc gatgccgcac
    12241 cgcggctcag cgcttccaga ccgagggcac ccgtaccggc aaagatatcg aggacagcgg
    12301 caccgcgcag gccgccgctg ttttcgagga tgttgaagag actttcccgc gtacggtcca
    12361 gcgtcggccg ggtcagcatc cccttcgggg acttcaggac ccgtcctctg gcactgccgg
    12421 atatgattct cataggatca cctgctcgta catagttaat tatacacgtt ttttattaca
    12481 tatcaatcta ttttttctta attcgttcga ccttcagttc cttgccgtgc atgaggcgga
    12541 taatgttgtc tttatggcgc acgacgacca gcagggctgc cagcaggccg aaaatcgtca
    12601 gcggcagagg ttcgttgaaa aagaacatgg taacgggcac caggatggcc gctacgatgg
    12661 aaccgaggga gacgatgcgc gtcagggcga cgatgacggc ccagacgcag aagacgatga
    12721 gcgtttccca cggggccagg aacaggatga tgcccaggcc cgtggcgacg cctttgccgc
    12781 ctttgaactt gaggaaaatc gagcacgaat ggccgacgat ggccagcagg ccgccggcga
    12841 tcatgaagta gatctgcgtg tccgtactgc ccaggcccga ctggctggcg acctggccga
    12901 ggtagacgcc gagcatcccc ttggcgacat cgcagatgag gaccagcagg ccgccgacgg
    12961 cgccgaggac gcggtaggcg ttggtcgccc ccgtgttatg gctgccgtag tccctgatat
    13021 ccgtatgata caaggccttg ccgatgacca gaccgcttgg gaaggacccg gcgatatagg
    13081 ccagtacaag gcagaggggg gcgagcatca tagggtgggc ccgcgcgtct ggccgcgcag
    13141 caggcgcagg agcggcggcg ctggcaagcc g
//
LOCUS       DAWCYS010000024        13006 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_319_length_13006_cov_9.659022, whole genome shotgun sequence.
ACCESSION   DAWCYS010000024 DAWCYS010000000
VERSION     DAWCYS010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_13006_cov_9.659022"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3122..3221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2823 a         3253 c         3565 g         3265 t
ORIGIN      
        1 agagggcggc ccgcctctcc tgttaggaga ggtggcccga agggccggag aggtttaacc
       61 tctcagtcag ctgtagctga cagctcccct ataaggggag ccattatacg agccacgaac
      121 cactaaaaag ggcttgtcgc acgtgcggca agcccttttt tcatgctata attgatgtaa
      181 aaaggggtga acacgatgga cttagcgcag ccgattttag agggcatcat cgctctggct
      241 aaacaatacg atattcaaaa agtcattctt tttggctccc gtgcccgtgg cgataattgg
      301 gaacgcagtg atattgacct ggctgtctgc ggtggaaatg tcacgcggtt ttctctcgat
      361 gtcgacgagg tcataccgac gctgctcatg ttcgatgtcg tcgatttaga tgaaccggtc
      421 cagcccgaac tacgagccgc tatccaacgg gaaggagttg ttatatatga aaaagtatga
      481 gaatttctgc aaagccttga ataacctcaa agaaataccg ccttattcga cggtcgtcca
      541 aacgggaatg gtttcccttt atgaaatctg ctttgaacag gctgggaaag ccatgaaaga
      601 agtattggaa aacaatggct atagccagca taagataggg tcaccgcggg cgattatcaa
      661 aatggcttat caggccaatc tgattgacga tgaggattgc tggctggagg cgttgcgtga
      721 ccggaacaat gtagcccatt cttataatga agaaattgcc aataatatca tcaaggacag
      781 caaggaaaag tacatcgcca tgtttgaaga gttgcagtgg gaaatagaaa aaaattggtt
      841 gtgaaaatga tcgggaggtg gaattttgcg aacgaattcg attttgatcg tcgatgatga
      901 tgaaaaactc tgtgccctgc tgcggtccta tttcgagcag gagcggttca tggtctatgt
      961 cgcctatgat ggcatgacgg cgttacatac actgcgcacc cagaagccgc agatcatgct
     1021 cctcgatttg atgatgcccg gcatggatgg cttcgaagtc tgccgccgca tccgccagtt
     1081 cagcgatata cccatcattt ttctgtctgc caaggatgat gaaacggacc gcctggtcgg
     1141 cctcaacatg ggcggcgacg attatgtgac caagcctttc cacgtcaagg aagtcattgc
     1201 ccgcgtctac agcctgttgc ggcggacccg tggtgaagta ctgcagcaga cgacgtcgta
     1261 taccatacgg gacctgacta tcgacaagga aaaatttacc gtcctgcggg gacagcagcc
     1321 cattgccctg acgccgacgg aattcaatct cctgctggtc ctggcctcgg caccggatcg
     1381 ggtattcagc cgcatgcagc tcgtggataa agtacagggc ggctatggct ttgaaggcta
     1441 tgaacggact atcgacacgc atatccgcaa tctgcgcaag aaactggagc ccgacccggc
     1501 ccatccgacg tatatcctga ccgtatacgg aatcggttat aaattcgacg gagggaatca
     1561 tgaagaaagc tgacagcatc gtcatccgcc tgaccgggat ggtctttatc ctgattttcc
     1621 tgactatcgc catcctgctc atcctggtca ataatcagat ggacagccat ttttcccagt
     1681 atttgaataa catgtcgcaa atgatgggaa acagccatca gggcatgggg atgatgcacg
     1741 gcatgatgca tgggccggcg gaaattacgt atatatcggc tgtccaccgg tcgctgctgt
     1801 gggtcggcct ggtcatgctg gccgtcagcg tcaccatcag ctacttcatt gtccgggaaa
     1861 tcatgcggcc tttgtcggcc ctgactgacg ccgtccagaa ggtcaaatcc ggcacgtatg
     1921 gccagaccgt acccgtcgag cgtcatgacg aagtcgggac cctgacggag actttcaacg
     1981 atatgtccca ggaactggcg aaaaacgata agatgcggcg ccgcctgttt gccaacatcg
     2041 cccatgaact gcggacgccc ctggccatct tgcagggcaa tctggaaggc atgatcgacg
     2101 atgtcatccc gacggataag aaggtcctcc tgtccatgga agatgagacg ttgcgcatgg
     2161 gccggctcat tcaggacctg cgcgacttgt cgctggccga aatcaacgag ctcaccctcc
     2221 ataaagaacc ttatgatatc aacgtcctcc tggaacgggc cgtgagcatg ctccagcctt
     2281 tatgcgacga aaaatccctg accgtccatt tggacttggc cagggattta ccgaaagtat
     2341 ccatcgatat agaccgtatc aatcaggtca tttataatct gctcaacaac gccatccgct
     2401 atatcgaagc gggatgtatg attaccgttt cgacgcaggt cgtcaccgta cagggccggc
     2461 cttatgtgca ggtccagatc gccgatacgg gcagcggcat tgccccagag gatttacagc
     2521 atatcttcca gtatttctac cgcagcgaaa aatcgcggag ccgcaagagc ggcggcagtg
     2581 gtatcggcct ggctctggcc cggcagttcg tccgcagtca tggcggcagg atatgggcgg
     2641 acagcgaagt cggcaggggg acgacgttta cttttatttt accaattcat agccagcgtc
     2701 ggcaatgact ttggcaaagg cttccgtcgg gatgtctttg ctggccgtga cgtcggcttt
     2761 tttaccttcc aagtcgacag tgacagccgt gacgccgtcc atggctgcca atgcatcgtg
     2821 tacgtgtttc tggcagtgct ggcacatcat gccttcgatt ttcaatgttt tttccatcgt
     2881 ttcatcctct tctttctttg gtgtagtgag attttgtaat gtatatccag cagtagtgat
     2941 gacttttttg aagtcttctg cggagatagc tttgtcagcc gagacatcgg ctttttggcc
     3001 atcgaggtcg acgacggcat cagtgacgcc gtccatggct ttcaggactt tcgtgaccgt
     3061 agccacgcag tgctggcaca tcatgccttc gatggacagg gtatagtgat acggagccgg
     3121 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcagtgctg gcacatcatg
     3241 ccttcgatgg acagggtata gtgatacgga gccggaacgg ctgccacttc gggaaccggc
     3301 gtttgcgtcg ctgcctgtgc agctttcggg ctactggttt ccggcgccga tgtttcacgt
     3361 gaaacatcgg tactgtgcgt cgtcttgaag aagcgcaggc gcagggcgtt catgcagacc
     3421 gtgaagctcg acagactcat ggcagcggcg ccgatcatgg gattgaggcg gatgccaaag
     3481 gccggataga ggacgcctgc agctaggggg atgcagatga cgttgtagaa gaaggcccag
     3541 aacaggtttt ctttgatatt cttgatgaca gccttgctca ggcggatggc gctgacggcg
     3601 tcgaggaggt cgctgcgcat gaggacggca tcggcgcttt cgatggcgac gtcggtcccg
     3661 gcaccgatgg ccatgcccag atcggctttg gccagggccg gcgcatcgtt gatgccgtca
     3721 ccaatcatgg cgactttatg gccttcggcc tggagggcgg cgatgtgttt ttctttatcc
     3781 tgcggcagga cgccggcgat gacctgggga atatgcaggc gctggcggat ggcttcggct
     3841 gtgcgctggt tgtcgcctgt gagcatgacg acgttgatgc ccattttggc aaagtcggca
     3901 atggcttcgg cgctggtctc tttttcgacg tcggctacgg cgatgatacc gatgatttct
     3961 ttgtcgtcag caaagaggag cggcgtcttg ccgtcgtcag ccagggcatt gaggctggcc
     4021 gtgatggctt tggtgtcgat atgggcttcg tccatgaggc gcgtgttgcc ggcataatag
     4081 gtcttgccgt cgatgacggc ctggacgccg cggccgaaga gggccttgaa atcgctggct
     4141 gtcctgccgg tcagctgatg gtcgccggca taggtcgtaa tagcttcggc caggggatgt
     4201 tcgctgcctt tttccagggc tgcagcgatg gcgacgaggt cttctttcgt gcctttcagg
     4261 ggaaggatat ccgtgacttt cggacggcct tcggtaatgg taccggtctt atccatgacg
     4321 accgtgtcga tggcatgggc cgtttccagg gcttcgccgg atttgatgag gataccgttt
     4381 tcagcccctt tgccagtgcc gaccatgatg gcgactggcg tagccaggcc cagggcgcac
     4441 gggcaggaga tgaccaggac ggagatggcc gtcgagaagg cgaattcggc gtcgacgccc
     4501 atgacgaagt accagatgag gcctgtaatg atggcgatgg tgatgacggc cgggacgaag
     4561 taacccgaaa tggtatcggc catcttggcg ataggtgcct tgctggcact ggcttcatcg
     4621 acgagcttga tgatctggct gatcgtcgtg tcgtcgccga cgcggcgggc tgtgatgtgg
     4681 atgaaacccg tcttgtttat cgtagccgac gtgactttat cgccgacttg tttttctaca
     4741 gggatgcttt cgccagtaat ggcggcttca tcgacgctgg tcgtcccttc agaaatgata
     4801 ccgtctgccg ggatgctttc acccggacgg atgacgattt catcgccgac gacgaggtct
     4861 tcgacgggaa tgaccgtttc gacgccgttg cgcaggaccg tggcctgttt cggcgccagg
     4921 tccatcaatt tttcgatggc cgtactggtc ttgcctttgg cgcgggcttc gaggtattta
     4981 ccgatgtcga tgagggtgac gatcataccg gccgattcaa agtacagatt ggtgctgtac
     5041 tgggcgacga gggcccagtc gccatggccc atgccccagc tcatgcggaa aatggcgaag
     5101 acgccgtaca gggcagctgc catggaaccc atgccgacca ggctgtccat gttcggggcg
     5161 ccctggaaga ggttgcggaa gccgttgaca taatacttgc ggttcaagta catgatcggc
     5221 aggatcagta agaactgggt aaaggcgaag gtaatggcat tttcatcgcc gtggaagaca
     5281 tactggaaac cttccggtac ggggatgccg aaccattcgt agaacatgtg gtgcatggcg
     5341 atgtacatga ccgggatgag gaagacgatg gaccagatga ggcggtgctt catggatttc
     5401 atttcttcct tggctatgtc agaggcgctc ttttgcggag cgccggcctg tttctggctg
     5461 ccgccttcgg ccgggctggc tccgtagccg gcgtcgacga cggcctggat gatgtcttgc
     5521 tcgctgacga cattttcatc gtacgaggcc tgcatactgt tggtcagcag gttgacgctg
     5581 gccttgtcga tgccgtccag cttgttgacg gctttttcca cgcgggacga gcaggcggaa
     5641 cagctcatgc ctgtgatgtt gaatttttcg tgcttcaacg gactcactcc tttatttcat
     5701 gagtttctga agggcgcaga cgagttcatc gacgacttcg tctttgcctt cacggatgtc
     5761 gtggacgacg caggacttga tatgctggcc caggagttcc ttattgaaag aattcaatgc
     5821 cgactggatg gcactgacct gagtcaggat atcaatgcag tagcggtcgt cttcgaccat
     5881 ctttcgcaca ccccgtacct ggccctcgat gcggttcagg cgggaaatga gcgatttata
     5941 ctctttgccc tctttgttgc gatacttatg tttctccgta tcgcagcagg gacagacttt
     6001 cttttcgtcc ataattgagt tctcctttca tttaaatacc cctatggggt acattgatat
     6061 aatataccga tatggggtat atgtcaaggg ggttttagcg gttagtagtt aggggctagc
     6121 ggatggtttt taatttgtag gggcgaccgc gtgaggcgcc cgctccggaa tatgcatgat
     6181 ttcctaatca gtttgccagg agaatcatcc caattcctaa cgggtcgccc acggccccct
     6241 acgaacgcta accgcggatt gttaaaaaaa ggactacgta tgatgatata agctctcata
     6301 cgatagtcct ttttctgtag gggcgtccgc cccgggatta tgcgatttca ctgtaggggc
     6361 gaccgcgtgg ggcgcccgct ccggaatatg tctgatatcc caatcagttt gccaggggaa
     6421 tcatcccagt tcctaacggg tcgcccacgt gcgaccccta cggtgcggcc atgaggccgc
     6481 cacgagccac taacactaac cactaaccac taaccattaa aaaaagctgc cgcaaagcga
     6541 cagctttttt actcctattt caacaatgct tttacagctt ctgtttcatc cgtagcgttt
     6601 tcagtaccgg agatgatggc accctggact acgatgcagt cgaggccttt gtccttgcgg
     6661 acctgttcga ctttggaaac gacgtctttt tcaatgggga cgatcagttt gttttccaat
     6721 ttgctcaagt tctgagccaa ttcacggccg aggttctggc gttcctggtc ggtcttcatt
     6781 ttcggagctt ttttagcgaa ctgttcgtta gcgtctttaa cggcagcctg catctgggcg
     6841 ctgactttcg ggaagtcctt atgggactgt accaatgtat cgaagttgac gacaccgacg
     6901 ccggaagcaa aggccgacga aacagcgacg aggctcatca ttcccataac ggcaagggat
     6961 gctaattgtt ttttcagttt cataatgtat tcctcctgtg ccgcagagcg gctgaattac
     7021 tctttcttac tatggttcta ttcgtatatt ataacaaact taccgataaa gtacaacctg
     7081 cacagaatca ggctttttcg tcgtcgcctt ctgctttgtc ttcttcatcg tcgtgaatat
     7141 aaatgtattt gtcttttttc atcttgtcga tgatttccgg tgccatggcg atgagttttt
     7201 caaaggccga agcctggtag ccatctttct tgatggagaa catttctacc cggtcccctt
     7261 tgatgaccag gatggcagct ggttccatct tggcaccgcc gccgctgccg gcactgctgc
     7321 gcccgttttc ggtcttgccg ttgacgccgc cggacccgaa gccaaaggtg acgtctacga
     7381 acggtaccag gatggcgtca ccgatgtgga cggcctggcc gacgacggtt tcgactttga
     7441 tcatttcttt gaaactttca aaaatttgtt ccaagttgtt cttgatgtac gagttatcca
     7501 tgttgttcct ccttgttcac agcgtgccac tcagcccata aacggcgtac aggccgggag
     7561 gcggcaaagg cggctgtaaa cagaagcagc actgccggat atagacggcc cgaagcacgg
     7621 acggtacagt catactgttc ctctacgaaa ttgaattgta agtctgtgac gctccccgga
     7681 cagatggcat agagggcacc ggccagcatc cccgtttcat gcggttcagg gaggccgacg
     7741 atgccggtca gggtgaagcg gcggatttgg ccgtggccga gcaggcgcga cagccaggtg
     7801 aagaaagccg ctaaaaagtc ggcattgaag aagctggcta agaaccggcg tatgggcggt
     7861 ttcttttttt tcgcttcttt cgcgtcactc tgtgctgggg catccggccc ttcctggcgg
     7921 agcaggtctt catacgtgag gcgctggctt tctttttcca gttcgtccca ggccttttct
     7981 tcctcttccg ctgtagggag cggcggttcg acagactctt tcacggcaag gctgcgcttc
     8041 cagcacatat gcagttcatg atgcggctcc tcgcccagcg tataggacca ggatgcatcg
     8101 gccaatttcc ggccccatcg gccctggcct tcgatgtgga aaggcgtccc gatatggatg
     8161 gtaagggcgt aggagacggg caggcagagg atgagcagta tgcagctgaa taagatggca
     8221 aggatgacga tgattgccat agggcagccc ccttttgtga aaataatccg ttacttttat
     8281 ttattcgatg aactcggggc gatttcctct tttttaggcg tcttgtttcg ctttttcttg
     8341 tttattgaaa atgcagtcca catcttcggc accgaagcag cgggaacagg aaatgcacag
     8401 ggccggttcg gtatcgcccg atttccagcg gttcggcagg tccggctggc gcaggtaggg
     8461 acggctcatg gacacgaaag ccaggtctgt cgattccagt gtatcttcga tatcttcgac
     8521 cttgcggaaa ccgccgacga cgccgacatc gattttgtct ttcagcatag ctgcggcttc
     8581 agaggcatag gctttgaaat acatcggttc ctttgtgcgg cggatagccc ggatcgggcc
     8641 cttgcgcggc agggacgatg tgttgccgcc gctgacttcg atggcgttta cacccatgtc
     8701 ggccagccag gtcatgacct gcttgctttc ttcaaaggtc atgctgccgt ctttcatgaa
     8761 atcggcacag ttcaatttga cccagaccgg ataatcggcg ccgacggcct ggcgcatggc
     8821 ggcgacacat tccaggagga agcgggcccg gttttccagg ctgccgccgt aggcatcggt
     8881 gcgggtgttg aaatggggcg acaagaactt gctggccagg tagccgtggg cgccgtggaa
     8941 ctggacgccg tcgaaaccgg cggccttggc tctggcagcg gctttggcga cggcttccgt
     9001 cagttcgtgg atctggtcga ctgtcagggc ctgcgattcc tgggtccact tgccgacctt
     9061 ttccgtaacg ccgctggggc tgatgataat gcgcgtttcc ccttccggga tttccggcag
     9121 gaggatggcg gaccccatga cgacgagctg ggcgatgatt tttccgccgt gctggtggac
     9181 gcggtggacg acctgggaca gcggggcaat acattcatca tcgtcgatgc ggatctggca
     9241 gtgctgacgc ggttccattt tggtagtagc catcatgccg gtaataatga taccggcacc
     9301 gccgtcggcc agattttcat acatttgggc cagcttttct gtcggaaggc cgtagttccc
     9361 cattccttca aaggtagcag aacggacaag gcggcacttc gcttcaatac cgccgatgtg
     9421 acatgtatca aataacatgg tagaaatctc tccttggtaa taaaaaatga atttcagttt
     9481 atcccgaata ttatacaata tttgaggaaa taagccaatg cattatttga ggaaataagc
     9541 caatgcattg ttttttgaac atttgggagt tcattttaaa aagggcttgt cgtcgtgcga
     9601 caagcccttg gctattcact tattgggatc atcaggagat tttttacctg tttctcgttc
     9661 atattcatcg aaatcaattt tgctttttga accgagttcc aatgcttttt tatagtacca
     9721 ttctttaaaa tccagctctt ccatgtagta ctgcagttgt tcaatctgat ccaggacaaa
     9781 ctgacgctgc tggcggatga gggctgacgc tgctggcgga tgagggcctg ccgttctttc
     9841 aggctttcat cgccgcgctg agccagttcg gcatattcgc ggatgcgctt gatgggcatg
     9901 cccgtatttt tcaggcaggt cagcaggtgg agccagcgaa tatccatatc gctgaagaga
     9961 cggataccgt ttttccgttc tacctggggc agcagccctt ctttgtcata atagcggatg
    10021 gcagacggac tgataccagt catctgcgcc gcttgtgaaa tcgtataaga catagtttat
    10081 ctcccttatt ttaaatactg ccggtagaag gcttcgattt tgtcgaaggg aatgacatcc
    10141 attttataat acaggtcggt gtgtacggca tcggggatga tcagcagttc tttgttgttc
    10201 cctttcagtt tcttgaacgc gtcttcgctg aagtagcggg agtgggcttt ttccccgtgg
    10261 atcatcagta cggcgctttc gatttcatca gcccgggaca gaaggggcat gttcatgaaa
    10321 tcgagcaggg aactgagagt tgcgcctgtg gtggagccga aggaccgggg gctgtagccg
    10381 cgtttttcct ggtagtagtc atgataatcc ttgacgaact gtggcgtatc ggctgttact
    10441 tctgtgggga caccgccggc cattttatag gtgccattcc ggtaatcttc tgtgcgctgt
    10501 gcgctgatgg ctttgcgggc ttccatccgt tcttttttta gggttgcttc atctttttca
    10561 tagtcgaaat agccattggc gatgacccgg ctcatgtcgt acatggtaga tatgaccgta
    10621 gctttgacgc gcgtatccat ggcagcatca ttcagagcaa agccgcccca gccgcagatg
    10681 ccgaggatgc cgattttttc cggggcgatg ttattgcggg tggacaggta atcgacggcg
    10741 gcagaaaaat cttccgtgtt gatatccggt gaggtcgtgt tgcgcggttc gccggcgctt
    10801 tccccggtaa aggacgggtc gaaagccagg gtgatgaatc cccgggccgc taattcctgg
    10861 gcatagcggc cggaaacctg ttccttaacg gcaccataag gaccggctac ggcaatggca
    10921 gcgagttttt caccttgttt catatttttc ggcgtataga ggtcgccgac taacgtaatg
    10981 ccatagcgat tatggaaggt aattttttgg tgagcgacct tcgcattttc tggaaacgtc
    11041 ttgtcccatt tattggaaag tgttgcagcg tcaatggttt cagccggtgc tttcgatgga
    11101 gctggggcct gggcggcata actgctaccg gcacctgcgc cgatgatgaa aagggagctg
    11161 cacagggcag cgaataccat atttttcatg atgcgttttc ttaatttcat aattataggc
    11221 ctcctgtcag aaaatttata gctcatttgt atttcatggc ctaagcataa accttaaagg
    11281 taactttaag tcaagggttt tctttagcgt gatttcttta taacccccaa aaccttttga
    11341 gttcattgac aaaatgggct taaatgatat acttctagta gaagtagtga atttttgcaa
    11401 ttgaaacacg aaagcccctg gaactgccat tccaggggct ttctctatgg tttaaaacta
    11461 tccgctaacc actaactgct agcccctaac ccctaacccc ttaaaaagga ttgtcgtcat
    11521 gcgacagctc cttttctgct ccttttgtaa agaaatatgt ttttagttgg cttcactaag
    11581 cgactatgat ataatataag tgtttgttaa ttttacaatt cacgaaggga tgttgaatac
    11641 atggctacag caatggtaat cggaacccaa tggggcgacg aaggtaaagg taagattgta
    11701 gattatttgg cccagaaggc cgatgtcgtc atccgctccc agggtgggaa taacgccggc
    11761 catacggtcg tcgtcgacga taagtccttt gcactgcgtc ttttgccgtc gggtatcctc
    11821 ttttccgaca aaacttgcat catcggcagc ggcgtcgttg tcaatccgaa agtcctcctt
    11881 gaagaaatcg aaggcatgac ctccaaaggc gtgcagatca gcaaattgga aatttccacg
    11941 cgtgcgcatg tcatcatgcc gtatcatatc cgcattgatg aagaagatga aaaactcaag
    12001 ggcgacgcta aaatcggtac gacgaagaac ggtatcggac cgtgctatgc cgacaaagtc
    12061 aaccgcgtag gcatccgcat cggcgacctc atggaccgcg atgtctttgc caagaaactg
    12121 cgcatcaata ttgaccttaa aaaccgtctc tttgaaaaat attatggctg cgaaggcttc
    12181 gattacgatg aagtcctcaa agaatacctc ggctatgccg accagatccg ccagtatgtc
    12241 aaggatacga actactcggc gaacctctat gtcagtgaag gcaagaaagt cctctttgaa
    12301 ggggcacagg ctgctatgct cgacctcgac catggtacat atcccttcgt taccagctcc
    12361 aatccgacgg ctggcggtgc ctgcactggt tccggcgtcg gtcctcgccg tatggaaaat
    12421 atcgtcggcg tcgtcaaagc ctatacgacc cgcgtcggcg ctggcccgtt cccggcagaa
    12481 cagctcaacg aagtcggcga atatctgcgc aacacaggcc atgaattcgg cacggttacc
    12541 ggtcgtcccc gccgctgcgg ctggctcgac acggctgtcg tcaaatatgc agccatgctg
    12601 aacagcctcg attacctggc tattacccgc ctggacatcc tcgatgacct ggatactatt
    12661 aaaatctgca cgggctataa atataaaggc cagctcttga aagaatatcc ggccagcctc
    12721 gacgtcctgg aaaacgtcga accgatttat gaagaaatgc cgggctggaa agcagacatc
    12781 tcgaaatgca aatcctatga tgaactgcct gaagctgccc gccactatgt cgaacgcatc
    12841 agcgaactca caggcgttcc cctgggcatc gtctccgtcg gcccgaaccg cagccagacg
    12901 atcatcctca aaaagatttt ctaaaaacaa ctgaattaca ccataattgt gaaaaggggc
    12961 tgtcgcatta agccatttcg gctttgtgcg acagctctct tttttt
//
LOCUS       DAWCYS010000025        12736 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_328_length_12736_cov_7.586310, whole genome shotgun sequence.
ACCESSION   DAWCYS010000025 DAWCYS010000000
VERSION     DAWCYS010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_12736_cov_7.586310"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2811 a         3487 c         3484 g         2954 t
ORIGIN      
        1 cgagggcatc cctgtctatc atggatgttt cctatttagt cttgttcacg gtctagggga
       61 cgtccggcga taacgcttac ggtccggaac gggcacgata gggggcggta gcatgacctt
      121 agcagaagga aaaatcggac acgactatat catcgaaaca ttggatttgc cggaccagac
      181 ggaaaagcgc ctccaggccc tgggcatgat tgaagggacg gaaatctccg tgctcaataa
      241 taagaacgaa gggacggtca tcatcaagat gcgcagcacc cgcctggcct tgggcaaggg
      301 catttcgtcg catatcggca taagggggaa agaccatgac tagcgaagta aaaataaagg
      361 ctggccagcc gcaggtccag aaggcaccgg tcaaggctga aaaaaaggaa atgaccgtcg
      421 ctttcatcgg caatccgaac tgcggcaaga cgacgctgtt caacgcctat acgggggcca
      481 acctcaaggt cgccaactgg cccggcgtta ccgtcgaaaa agttgaaggc gccatcaatg
      541 cccacggcta tcacattcat ctcgtcgacc tgcccgggac gtacagcttg acgtcgtaca
      601 ccatggaaga acaggtttcg cggaatttca tcctcagtga cgatgtcgac gtcatcgtcg
      661 atgtcatcga cgcgtcggcg ctggaacgca atctctacct gaccttgcag ctcctggaac
      721 tggggaaacc cgtcgtcgtc gccttgaaca tgatggacat cgtcgaaaaa cggggcatgg
      781 aaatcgactt gcaccgcctg ccggaaatgc tcggcgtgcc ggtcatcccc gtatcggccc
      841 tgaagcgccg cggcctgagc gtcctgctcc atgcggccgt gcaccacaag gaccacgtcc
      901 acccggatcg gctcatccac aaccattcag accataccca tcatcagcat aatcatcaca
      961 gtgaattcgc catggtctac agcgacgcca tggaagaccg catcgacgcc gtgcgccagc
     1021 tcctgcatga caaatatccc gatttgtaca atcaccgctg gcatgcgctg aagctcatcg
     1081 aaagggaccc ggaaattacg gccaagtatc ccgtcgacga cgtccatcac gtcctggacc
     1141 gcagttatga aacggatatc atcaccgaaa aatatgcctt catcgaagaa atcatccatg
     1201 aagtcgtcgt caaccaggcg tcgaaagcag cggcaacgga ttccgtcgac cgctacttga
     1261 ccgataaatg gctgggcatc cccatcttcc tggccatcat ggccatcgtc tttttcctga
     1321 ctttcttcat cggcgatatg gtccaggatt ccttccaggg ctccattgat actttatcgg
     1381 ccctggctga aggcggcctg acttcgctgg gggctgacga tatgctcatt tccctggtct
     1441 gtgatggcat catcgccggc gtcggcggca tcctgacctt tttgcccaac atctgtatcc
     1501 tcttctttgc cctggccatc ctggaagaca gcggttacat gtcccgcgtg gcctatgtca
     1561 tgaacgatat catgaaccgt ttgggtttgt cgggccgggc cttcatcccc atgatcctcg
     1621 gctttggttg cagtgtgccg gccatcatgg cgtcgcgtgc tctggaggat aaacgcgacc
     1681 gctatcggac gatgctggtt acgcctttca tgtcctgcag tgcccgcctg cccatttaca
     1741 tcctcttttc gggcatgttc ttcccgcaca atgccatgat tgtcgcctat tccatgtacc
     1801 tcatcggcat cgtcgtcgcc ctggcagccc tgaaggcgat gaatctcttc agtcatgaga
     1861 aaaaggaaaa cgacctgctc atcgagctgc cggaatataa gcggcccagt gcccatacgg
     1921 tctacgtcta tgtctgggaa aaaatcaagg attacctgag ccgtgccggt acagtcatct
     1981 ttgccgcttc catcctcatg tggttcatcc tcaacttcgg ccctgccggc tatggcgata
     2041 tggccgacag tttcggggcc atcatcggca aggccatcgt gcctttcttt gcgcccatcg
     2101 gcctggggta ctggcagatc tgtctggccc tcatcgctgg catttcggcc aaggaagtcg
     2161 tcgtatccag tttcgccgtc ctcttcgatg gcgtcaacat caattctccg gcagccctgg
     2221 cggctttggc ctctatgatg agccaaggcg gctttactca gctcaatgcc tactgcatga
     2281 tgctcttctc gctgctctat attccctgtg ccgccacgat agccacgatc cacgccgaat
     2341 cgcacagctg gaagtggacg gctttctccg tctgtttcca gattgccgtt gcctgggtcc
     2401 tgaccttcgt cgtctatcag atcggaagtc ggatttttta atcactaata actaaggagt
     2461 gcagtatgac agaacctatg ataaaaacgg atcatctcag ccatacctat gaagacgaag
     2521 acggcaaggt ggtctatgct ttgaaggata tttccctgga aatcggcaag ggcgagttcg
     2581 tcgccatcat cgggaccaac ggttcgggca agtcgacgct ggccaagcat ttcaacgtcc
     2641 tcctgacgcc gacgtccggc acgtgtgaag tctgcggcat gcggaccgat gaccccgaca
     2701 atatctggaa gatacgccag cacgtcggca tggtcttcca gaatccggat aaccagatcg
     2761 tcgccgccgt cgtcaaagaa gacgtggcct ttggcccgga aaacctcggc gtcccgtcgg
     2821 acgagatcaa gacccgcgtc agcgatgccc tgcgccgcgt caacatgacc atttatgcca
     2881 agcacggacc gcacctcctg agcggcggcc agaaacagcg cgtcgccatt gccggtatcc
     2941 tggccatgca gtgcgactgc atcgtcctcg acgaaccgac ggccatgctc gaccctgtcg
     3001 gccgcaagga agtcatggat acgctccacc agctccatga cgaaggcatt accatcatca
     3061 tcatcaccca tttcatggaa gaagccgtcc aggccgaccg ggtcgtcgtc gtcgaccagg
     3121 ccgaactgaa gatggacggg gcgccgcgtg acgtctttac gcacgtcaaa gagctgaagg
     3181 ccatgggcct cgacgtaccg gtagcggcgg aactggccga acggctgcgc cagaaaggcc
     3241 tcgacctgcc ggactccatc attaccgagg aagaattagg agatgcatta tgtcaattaa
     3301 gttaagcaac gtgacctata cctacggcat cgggacgcct tttgaaaaga cggccctcta
     3361 cgatacagat gtcgaaatcc atgaagggga attcgtcggc atcatcggtc ataccggatc
     3421 gggcaagtcg acgctggtcc agatcctcaa cggcctcatc aagccgactt ccggaacggt
     3481 gaccgtcgac gggacggata tcggcaagaa gacgaaagaa gccatggccg tgcgccataa
     3541 agtcggtatg gtcttccagt atccggagta tcagctcttt gaagaaacta ttgccaaaga
     3601 catcgccttc gggccgcgca atttcggtct gagcgaagaa gaaatcacag aacgggtcaa
     3661 agaagccatg gacttcgtcg gcctcgatta taagacctat gccgaccgtt cgccgttccg
     3721 cctgagtggc ggccagatgc gccgcgtcgc cattgccggc gtcatcgcca tccatccgtc
     3781 gtatctcatc ctggacgaac cgtcagccgg cctggatcct atcggccggc gggaaatctt
     3841 ttcggaaatc cagcgctggc accaggaaaa gggcattacc gtcatcttgg tatcgcacaa
     3901 catggacgat atttcccgcc tggccagccg cctcctggtc ttgtcccacg gccatatcgt
     3961 cctcgacggc gaaccgctga acatctttgc gactcagcag caggccctgc acgatgccgg
     4021 cgtgtccgtg ccgcctgtaa cgggtgccct ccaatatctc cagggccggg gctttgccgt
     4081 cgacgaccgg gtacggacgg tcgaagaagg ggcacaggct atttttgact gcctgaaagg
     4141 aggcaagggc catgctcact gatattactt tgggccagta ttttcctggc acgtcttttt
     4201 tgcataaact cgatccgcgc atcaagatcg tcgctacgat gatcttcatc gtcgccatct
     4261 tttttgccca ttccctggca tcctatgcca tcgtcacggc cttcgtggcc ttgaatttcg
     4321 ccatttcccg cctgccggcg aagttcatcg tcaagtcgct gaagcccttg tgggtcatca
     4381 tcattttcac catgggcgtc catatcttca cgacgccagg cgatgtcctc tggcagtacg
     4441 gcattttcca catcaccaaa gaaggcctgt atcaaggggc cctcatgacg gcccgcctgg
     4501 tcttcctcat cgtcttttcg tcgctgttga cgtatacgac gtcgcctatc gtcctgaccg
     4561 acggcatcga acacctgctc aatcccttta agcgcatcgg tgtgccggct cacgaactgg
     4621 ccatgatgat gaccattgcc ctgcgcttca tcccgaccct tttggaagaa acggaccgca
     4681 tcatgaaggc ccagacggcg cggggtgcca attttaccag cggcagcctc ttgcagcgcg
     4741 gcaagaacat gatccccctg ctggtaccgc tcttcgtcag cgccttccgc cgggccgatg
     4801 acctggcgac ggccatggaa gcccgctgct accgcggcgg tgaagggcgg acgcggatga
     4861 atgaattgtc ctatacctat cgggacggca tcgccatggc ggctgtcctg attgtcacgg
     4921 ccgtcctggc cgctatgcgg tggttatgat gaagaagaat atctgcctcg tcgtcgccta
     4981 tgacggtacg gatttccacg gcttccagcg ccagaccaac gccgagtcga tccagggctg
     5041 catcgaacag ggcttgtcgg ctattttcca gacgcctatt acgatttatg gcgccgcccg
     5101 cacggatgcc ggtgtccacg cccgcggcca ggtcgtcaat ttctatggtc aggaatcgat
     5161 tccgacggaa aagattccct atgccatgaa aggctatctg ccgccggaaa ttgccgtcct
     5221 gtcggcccgg gaaatgccca atcgcttcag cgtccgccac gataatgtgg gcaagcatta
     5281 ccgctattcc atcgacaaca accgcatgca cgaccccttt ctcctgcgct atgcctggtt
     5341 catccgcaag cccctggacc ggcaggccat gcgggaaggg gcggccatcc tcttggggac
     5401 gcatgatttt tctgcttttg aaggccagaa tacgacgccc atgaatcctg tgaagacgat
     5461 gtattccatt accctccatg agcggggcca tgtcctagac atcgacgtcg tcggcgacgg
     5521 cttcctctac catatggtgc ggaacattgc cggcggcctc gtcgacctgg gcctgcaccg
     5581 cctgacaccg gagcgcttcc gggaaatcct cgccagcggc gaccggactc agctcggggc
     5641 gacggcaccg gcccagggcc tgtgcctgga gaccgtcttt tatgatgaag gggagctgaa
     5701 agggcgcatt gccgaattag aaggagaatg agccttttta cagcgctgat gggcataata
     5761 tgctataata aaagagaata gtatagttcc gtttttttca ggcggaggtg aggaccatgc
     5821 tgaccagctt actgaaaacc attactaaag gaaaagacct tagcgaacgg gaaagcgcta
     5881 tcgtcatgga gcagatcatg gaagggacga tttcagacgt ccagttggct gcttttttgg
     5941 cagccttgac gacgaaaggc gcttcagagc gggaaatcac ggcctttgcc cggaccatgc
     6001 ggaaatacag tatgcatgtt ccctgtacga tggacgtctt cgacatcgtc gggaccggcg
     6061 gcggccggac caagaccttc aacatctcga cgacggcgtc tttcgtcatc gccgccggtg
     6121 gcgtccgcgt cgccaagcac ggcaaccggg ccaagacgtc gcgcagtggt tctgccgacg
     6181 ttctggaagc cttaggggcc aatatcttcc tcagccccga aagctgcctg gatatgctgg
     6241 ggcagatcgg cctctgttat ttctatacgc gttactacta ttatatgatg aaacgtatcg
     6301 atgccgtccg cgaacagttg ggtatctata cggtcttcga tgtcctgcgg cccttgacca
     6361 atccggctca tgcagtctat gagatcctcg gcgtcaatgc gccgcacctg gtcgaaccca
     6421 tggcccatgt cctggctgcc ctcggcgtcc gccacggcat ggtcgtctat ggtcaggacg
     6481 gcagcgatga aatctcggca tcggcagcga cgacgatttg cgaatttacg ccagaatcgt
     6541 tttcgacgta tgaaatcagg ccggaagatt tcaacctgcc cagggcttcg cgggacgaac
     6601 tgcgcggcgg cctgcccaag cacaatgctg ccattacccg cgccgtcctc gacggccgca
     6661 agggcggggc ccggacagcc gtcctcctca atgccggcgc cggtctctcc gtcggcggca
     6721 gtgtcctcca cctctatgcc ggcgtccgca aggcagcccg cctcatcgac agcggcctgg
     6781 cccggcagaa actcgatgac ttcctcatcc tgagccagcg cctgggacag gcggaaaaag
     6841 tgaagctcat agcagacagg gacccggaag atatggtata ataaagggta cgaaaaaatt
     6901 cattattacg tgaggaccgc atttctatga atgataaata tttaggctgg ctccttcata
     6961 tcggcggggg catcttagtc gtcttgatgt ttttatcgat tcagctcatg gcacctgatt
     7021 tttattcgat catgtggcat ctgaccgtca gtggtgatct ggatgggtta gccgattaca
     7081 ttgcctcttt tggcttctat gccatcggca tcagcgtgtt catgattgcc tttgtcaatg
     7141 ccatcggcct gccgtccatt cccttcctga ccgtcaacgg catcatcttc ggcctcattc
     7201 cgggcatcat catttcctgg atcggcgaag tcatcggcat tgaaatcagc ttccgactga
     7261 ctcggacgct gctccgcaag caggcccaga aggtcatttc caagagcaag atgctggaaa
     7321 aactcgattc ttacagctgt atcaagacag tcatgatggg ccgggccatc ccgtatgcac
     7381 cgaatgtcgt cgtcacggcg ttcagtgcct tgagccatat ttcttaccgc gaccacttca
     7441 ttgccaactt cttcggcaag atgccggccg tcctggtcga agtctggctg ggccatgacc
     7501 tcctgcgcat ccaggaacac tggggccgcc tgctcatatt ggtagccgtc gtttctttca
     7561 tttatggcat catctggtgg cgcaagcgtc acataaaaaa aaaccgatat catagggtga
     7621 agtaaaaaaa aggagctgtc gcatcgcgac agctcctttc aggttgttag ttgttagcgg
     7681 ataggtttaa ttttttactt ttttacgaga tttcctgttc tgacgatttt ttatcgtcct
     7741 ggacgatatc agtccataac ttatcggctg tcggctgcca gtgcttggca taggctttgc
     7801 atttagcaca gaggtgttca accgattctg gtgactgcat gtccgtcgat ttggctcccg
     7861 tccgtttgac catatcgacg agggattggg gattttccaa catcgggcag gggcggagat
     7921 gattgtggtt gaacggctgc ccttttgcgt attccttaaa taagggctgc tggagacagg
     7981 tgatgaggtc ctggtcgttg atgttggcac tagagtagtg gataaagacg cacggttcga
     8041 cgtcgccgtt aggattgata tggcagtagt tgcggccgcc agcgacacag ccatcaatga
     8101 actggccatc gttctggaag tcgatagcaa aaatgggttt gccgcccttt acgctgcgga
     8161 tttcacggat acgccgtacc atataagcac gctgttccgg tgtgggcagt aaatcgacag
     8221 aggcatcgtt gccgacgggc atatagtgga agtaccaggt aaaccaggct cccttgctga
     8281 tgaggaggtc gaggaaggta tcggatgtga cggtctggat gttcttacgg gtatagcaga
     8341 tggaagtacc gaagacgata ccgtactggc gcagtaggtc catggcgtgc atgaccttgt
     8401 cgaagacgcc ttcaccgcgg cggctgtcgt tgacttcgcg gaagccttcg acgctgaggg
     8461 agaagctgag gttgccgacc ttgacgactt cctggcagaa tgcatcgtcg atgagggtcc
     8521 cattggtaaa ggcgtggaat tcgcagtccg gatgtttccg acaaagtttc aggatgtccg
     8581 ctttgcggac cagcggttcg ccgccagtga ggaggtagaa atggcagcct acttctttgc
     8641 cttggcttac gattttatcc atgacatcgt aggacaggtt cagggtgtga ccatattcgg
     8701 cagcccagca gccgatgcag tgcaggttgc aggcgctggt cgggtcgaag aggatggccc
     8761 agggaatatt gcagtgatat ttggctttgg cttcgtcccg cgtacgcaga ccgtagaagc
     8821 ccgattcaaa gcccaggtcc atgacggctg tacggactac gttggggttg agtgtatcca
     8881 gggctcggtt gaggaattgg aaccacttgc cgccttcgtc aatgagcttg cgggcattgg
     8941 caaaagattc ttcttcaaat aagtcaccga agaaggtttc cataatcgtg gtcagtcgtt
     9001 tcaaagtctt cttacgggtt tctggatttt tttcggccat gatgcggttg accaggaggg
     9061 cgaccgattc tttttgggct ttatacatga gtttattcaa gggaatcatc tcctgaggag
     9121 tttaatgaag taatgattag cggatgatga ggacgggaag gcctgaatgg gaggatacat
     9181 agcggccgac gctgcccagg aggacgcttt ggatacggct caggccgcgg ctgcccatga
     9241 cgatgaggtc cgtttccgta tcggctgcgg tctgtaagat gatcggaccg ggcgagccga
     9301 ttttatacag gcattgcaag gggacttgag atgacaattc ttcctggcag cgcaggaagg
     9361 cgttatgacc cttggtccgc atgtgagcgg ctaattcctg taatttatgc ggttccagcg
     9421 tattggggat ggccgtatcg cgcaggctgt cgctgacgct ggcgacgtag aggacggtca
     9481 gggaagcgcc atctcctttg gccaggactt cggcttgttt cagggcctgt aaagacgatt
     9541 cagacccgtc tacgggcacc aggatatgcc ggtatgggcc tttcttttca tcgcgggcca
     9601 agaggaccgg gcagggtgcg tgggtgatga cgtaagtgct gacgctgccg agaaaaaaat
     9661 acttgaagaa ctcgccgcac ccgctcacca tgcagatgag atctgacgat tgggatttct
     9721 gttctgccag gatggccgct tccggggctc tgagttcaaa aatagactgg actgacaggg
     9781 aagcgggtat catggcggca aattttttta acagtcctgt gctgttggcc tgggccgaag
     9841 ccgtcatttc atccatagtc agttcactcg tattttccat ttgcccgaac tggttgatga
     9901 tatcggccat attggcgacg ctcagcaggg taatggcacc gccagtgatc ctggcgatgt
     9961 cgatggcgtg ctgcaaggct tgtttagaat gatcggatcc gtcgataggg acgaggatat
    10021 gctgatatga tttcataaaa atacctcctt caagggtacc tttaggatag ccttgaagga
    10081 ggctggcgtc aaagtggttt tgccgtatac gaactatagc tcttgtgtta taattaggta
    10141 tacgaatatt ttttgttaca taaaggagct tttatggagt tggaaagacg tgaatatgaa
    10201 tatatccgta aaattgctga gacagggagt ttcatagcgg cagccgaaaa attatacatc
    10261 acccagccgt cgttgagcca gttcatccgc cgcatcgagc gccatgtcgg tgcggaactc
    10321 tttgaccgtt cccatcagcc tgtgaccttg accgaagccg ggcgtatcta tctggacatc
    10381 gaagggcaga tccaggactt gtgcaaggtc cgggaacagc gtatccagga tttggggcag
    10441 accgttggcg gcaccgtgcg catcggcagt tccaattatc gcagttcgac catcctggcg
    10501 cgggctattc ccatcatcaa cgaacgctat ccgcacatcg atatctgcct ggaagaaggg
    10561 acgacgcggg aattggaaga tttcgccatc cagggaaaga cggatttttc tatcgtcgtt
    10621 aagcctacag ggcataggga cttggattat atcgaacttt tccgggaaga attgctcttg
    10681 gctgtctcgc cggactgggg cctggggcgg gaaccgctgc atgacgaggg cctccactcc
    10741 cgctatcctg tctttgatta tcacagcctc gacggccatc ccttcctggt catgaaagaa
    10801 ggccaggaac tgcgccattc cttttttgcc cttgtcgaag ccctggatat atcgccgaca
    10861 gtggccctgg aaacgacgga catggccacg gcccagaccc tggccggcat cggcgtcggc
    10921 gcggctatcg taccgggtgg cctggctctg gccaatatcc cagctgtgga accgaagtat
    10981 ttttccctgc gggcctatct ggacgactgg gatatcgtcc tggcctataa acggggacgt
    11041 tacttgtcca aggccgataa agccgtcatc cgcgtcatct gtgacgtcgc tggttgaaaa
    11101 gcgctgtcgc gtagcggctt ctgccatccc ctaaccacta gctactaacc actaaccact
    11161 aaaaaaagcg ctgtctttat aagacagcgc tttttgcatt cccaaacgga ttattttgcg
    11221 aagtaacgag ccaggaggcc atcgaataca cggccatgac gagcttcgtc ttttgccatt
    11281 tcatgaacag tgtcatggat agcgtcgagg ttgagctgtt tagcgagggt tgcgatttct
    11341 ttcttgcctg cgcaagcgcc ctgttcagca gcagcacgca tttcgaggtt ggtcttggtg
    11401 tcgtccgtaa cgacttcgcc gaggagttca gcgaatttag cagcgtgttc agcttcttcg
    11461 aaagcgatgc gtttgtaggc ttcagcgact tccggatagc cctggcggtc agcgacacgg
    11521 ctcatagcga ggtacatgcc gacttcggag cattcgcctg tgaagttgag gtggaggcct
    11581 tcgaggatgc gttcgtcgac gcctttagcg atgcctacgc gatgttcgtc agcgtattct
    11641 ttgacaccag cgacgagttc cgtgaatttt tctttcggag cgccgcactg aggacacttt
    11701 tccggagctt ccgtaccttc ataaacaaaa ccgcagatac cgcatacaaa ttttttcatc
    11761 attcattcct cctgatatta taatacattt tgaataaaat ggatattaac tgaatactaa
    11821 caaataactt ttatttgtta tggctacatt gtaacagact gatgggggaa aatcaagagt
    11881 aattttgtaa aacttttaat tgaaaataat tattgattaa aaaataaggc cttggagggg
    11941 cgaggcggca ccattttatt gcaatttata catgagtctt atataatgga ggtagtattc
    12001 agaaaaagcc aagacgatac gattagcata gaaagagact gaattttgga ggttacgcct
    12061 atgtggaaga aaaaagtagc atgcatcctg gctgtcatgg ccttgtgtgg gaccatggct
    12121 gccggtgcgg ccgattatac gatcctggaa aaagcggata aagtcgaaac gacggtctac
    12181 gggacgaccc agaccggttc cctgaacgac cgcatcgcgg cgctggacaa gctgctcaac
    12241 ggtcagtcga cggtatccgg cagtctgcag gataaaacta atgccctata taaagatgta
    12301 tatgggaatt ccggttccga tttgtccctg ttgacggcag tcaacctcat gcagtggcag
    12361 tattccggcc agattaccga tgaacccctg ctagtccgca tcgaatcgct ggaacagagc
    12421 attgacggca agaccatgag cggctccctg gaaggccgtg tcctggccct gcgccgcacg
    12481 ctcctgggca ataagaagta cgtctcccag accgtgacca ttccggccaa tacgctggtg
    12541 acgatgacca atatcgacgc cctcaactcg aagaccattc aggaaggcga cgtcgtccgc
    12601 tttgccgtgg ctgatgatat ctgcgtcggc gacgtcatcg ccattccccg cggcatggaa
    12661 gcgacgggga ccgtcaccaa ggcccggaag tccggccgct ttggcaagga cggcaaaatc
    12721 gaaatcacct atgaca
//
LOCUS       DAWCYS010000026        12736 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_327_length_12736_cov_10.732355, whole genome shotgun sequence.
ACCESSION   DAWCYS010000026 DAWCYS010000000
VERSION     DAWCYS010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_12736_cov_10.732355"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3300 a         3436 c         3213 g         2787 t
ORIGIN      
        1 aatatgagcc ttattggcaa ttaactccct ttgacttgac cgtacttctg caaagcctcg
       61 atggcatagg cagaacaagt cggataaaac cggcagcacg gccggtgaag gggcgatatg
      121 aacaactggt aaaaccggat aagggctatg aaacaccgtt tcatgtgtca tcacttcttt
      181 ttcaataccc tggcatgccg gaataattgc aggatgcttt gttctgcttc tttgtatccg
      241 gcgtatttta aactgctgcg gccgatgagg accaagtcga acccgtcgcg caggtcgaac
      301 tggtgcagac gatagacttc tttcatcagg cggcggcagc ggttgcgttc gacggcgcag
      361 ccgattttct tacccgttac gaaaccaatc cgcgtcggct gtttcggtga gcgcggcagg
      421 acgtacagga cgacgcggcg gttgacatac gacttgccgc ggcggtatac gagctggtac
      481 tcacggtttt tgcacagccg ttgttctttt gttagttcat acatacgcaa attgtcctaa
      541 aaatgccaga aaaacagaaa aattccgcca accggaactt ttctgtttat ctcgtaataa
      601 ttaagcagaa agtttctttc tgcctttcat gcggcgtttt ttcaaaacca tgcggccgcc
      661 tttcgtcttc attctttcgc ggaagccgtg agtcctcttt ctccacaatg tattcggctg
      721 atatgtctgt ttcaagctag acacctcctt tgtcgtcatt gacagtaaat gaatttacac
      781 cataacaggt taattatagt aagcgaagcc ccggctgtca agccctattt tccaagtatt
      841 atactgtttg cgcatacctc atgtataagt attccgtaaa aattttcttc ccttaatttc
      901 ggttttgttg taaaatgtgg atatataaat aagttgtcaa caatctatcc acattttgtg
      961 gataagtgga aaagaaccgg aaattcgatg attatcgtct ttccgccttt ttgtgtcctc
     1021 tttgtccacg ttatccacaa ttcaggctat acacatatac actttatatg gtacactcta
     1081 tgtctcgcac aattttacgc acagattgga aggattatta tggacctgct cagcttatgg
     1141 gaaaaaatgc tggctaacct gaaacaagtc ttgccgaaag agatttttac caactggttt
     1201 gaaggcaaca tcattcccta ctcctattcc gaagaagaaa atctcctcat attagacaca
     1261 atgacgaatt ttctccgcga ctacgtcagc aaaaaataca cggatatcct ggaaaccgtc
     1321 gcccaggacg ccatcggccg cccgacaagg gtccagctga ttacgagcca ggaaaagaag
     1381 cccgctcccg aaagccagcc agaagccgtc gctgaaacga tcaccctgcc gaaaaagccg
     1441 gcagcgcctc ccgtaccggc agccgctgcc ccggccggag acgcgcagtc cgtcccgtca
     1501 ggacagacag aatgggtcca gcctgaattg cccatcagtg acggtccgtc agcccccatg
     1561 cccgtcgtca aagaagccgt ccccgtcacg cctgatgaaa tcccttataa taataattta
     1621 aataaagatt atacctttga taacttcatc gtcggcaata gcaaccgcgt cgccacagct
     1681 gtcgccaagt ccattgcagc cgcaccggcc cagaaatgga accccttcta catctacggc
     1741 ggcagcggct tgggcaagac ccacctgatg cacgccatcg gccacgaatt gctgaagaac
     1801 ttccctaaca tgcgcctgtt gtgcatcacc agtgaagact tcgtcaacga attcatccag
     1861 tgtatccagg acaaaaacac ggaaagtttc cgccgccgtt accgcaatgt cgatgtcctc
     1921 ttcgtagacg acatccagtt cctgggccgt ggggataaag aaagttcaaa agaagaattc
     1981 ttccacacct tcaacaagct ctatcaggac aaaaaacaga tggtcttcac cagcgaccgt
     2041 ccgccacggg acatcaagaa actggaagaa cgcctgcgtt cccgctttga aaacggcatg
     2101 gtcacgcaaa tcgagccgcc tgatttagaa acgcgcacgg ctatcctccg ggcctgggca
     2161 gaaaaagaaa atatcacctg cgacctggat gccctcaatt acatcgcagc caatgtcagc
     2221 gaaaacatcc gaaaactgca cggcgccttc atccgcgtcc tcctggaagc atcgcagtcc
     2281 aagagcaacg tcaccctgcc tttagtaaaa cacgccatcc attacattac cgacgcccag
     2341 gatgaaaaga aatatatcac cattgacgaa atcacgacgt acatctgtca acactataac
     2401 atcaattata aagagctcat gggcaagaaa aagaccaaag acatcgccct gccgcggcag
     2461 attgccatgt atatgtgccg cgaactgaca ggaaatacgt atcctcacat cgggacagcc
     2521 ttcaacggcc gcgatcatac gacagtcatg cacgcctgct cgcagattcg gaagaaaata
     2581 gacaaagaca gccatttcaa agaacatctg gaaaagatga tgaatgaaat ccgcggtgtg
     2641 gataactagg ctgataaaat tgtacataca ttgtggataa gtcaaaaaaa gaaatccaca
     2701 gtggaaatgt ggataaaaaa tgactatttg tccacacgtt atccacagct gtggacagct
     2761 taaaatcgct gtctttttcc acttatccac aaattcacag cccctattac taagactact
     2821 attttttaat tcacatttaa ttaatttatt cagcgcagaa aagaggatac tccattgaga
     2881 ctaaaactga aaaaagaaga tctgaacaaa ggcctgcagg ctgtccagaa agtcgcccag
     2941 aacaaaagca acaatatatc ttctgaaaac ggcttgctta tcaaagccct ccatggtatc
     3001 atcgagttcc aggccaatga ctacgatatg ggcattaaga tcaccgttcc cggtatcatt
     3061 gaagaaggcg gtgaagcttt tattgccaat ccttatttaa tggaattgac ccgccgcctg
     3121 cctagtgatg aaatcgaaat catcaaggaa gatgccgata cacagctgct catcaaaggc
     3181 ggcaaactca agtttgaatg tctgaccatg aatcccgatg atttcacaga agtcgaaatc
     3241 gttgaaaacg gctatgccag ctttacgacg acgagcgtcg tcatgaagga cctcatcgac
     3301 aatacgtcct atgcctgcgc tactgatgtg gcccgcccaa ttttcatggg gaccttcctg
     3361 gacgtccagg gggatcagat gaccatggtc gccactgaca cgcaccgcct ggcattgaag
     3421 acggctaccc tcgatgcgcc tgtggataat cccttacagg ccgttatccc cagccgattg
     3481 ttatcagaaa tttcccgcca gctgccgaca gatatcccgg aaaccgtcga aatcacggca
     3541 gtccgcaatt acctggccgt acagttcggc aatgtctaca tccggatgcg gctcatcgaa
     3601 ggggaattcc ccaattatcg gcgcgtcatc cccaatgact tcaagtgcat cgtcacggtg
     3661 gaccgggccg aattcacggg cgctgtcgaa cgcgcttcta tcgtcgccaa agatgcccag
     3721 tacaatgtca tcaacttcat cgtcgccgac gaccagatca aactcatgag ccagcatcct
     3781 gactacggta cggtcgaaga ctacgtcccc tgccatttgg aaggccaggg cctggatatt
     3841 tcctttaatg ggaaattcat cctcgacatc ttgaagcatt gccatgattc ggaagtcatc
     3901 ttgaaatcca aggaaaacag ccccatgctg gtccaggata aagaagatac ttcctgtgta
     3961 tttgttgtaa cgcctatgag gacaaaataa tggaaacagt aaacattacg acaccgatga
     4021 tccagctcga ccaattcttg aaatgggcca gtattttaca gagcggcggt gaaatccgct
     4081 ttctgctgga cgaaaaaaag atttttgtaa atggaatttt atgcactgcg aaacgcaaaa
     4141 aactctatcc tggagacaaa gttgaaatta aaggtatcgg tacatatttg attaaaggtg
     4201 aataattatg atgattgaat cggtccggct ccaccgtttc cggaattatg atgatgaaat
     4261 gattaccttt cccaaggcca tcatcctcct ctatgggaag aatggccagg gcaagacgaa
     4321 tctattggaa tcattatata tggggggcat cggcaagtcc taccggggca tcgccgatgc
     4381 cgacctcatc agctggaacc aggaccaggc cagcatcatc atccagtttc agcgcagtga
     4441 tacgcagcag cagctgaaaa tcatcttatc ccggacggcc aagaaagaac tctgggtcaa
     4501 tgaaacaaaa gttccccaaa aggaactggt cgggaccctc aacgaagtcc tgttctcgcc
     4561 ggacgacttg cagctcatca agggcagccc gtcgctgcgg cgccgtttca tcgacatgga
     4621 aatctcccag gtcagccggc cgtactaccg ggaactgctg cagtacaacc gggccatttc
     4681 ccagcgcaat ctcctgctga agaagatgaa atatgaaggc atccatcctc tcgatgaatg
     4741 ggaccgccag attgccggct ttgcagcttc gctggtcgcg aaaaggctcg aagccctgca
     4801 caagttgtcc tttttggccg gcgtcctcca caagcgtctg agccggggga cggaaatgct
     4861 gaacctaaca tatgttcagc cctatggcgg cggcgaccgg gaccatcaga ccaatccgga
     4921 atggtactat gccctcttgc agcgccatct cgaagaagac gtgcgccgtc aggcgacatc
     4981 ggtcgggccg caccgggacg acctggtttt ttccgtcggc ccggcggatc tgcggaaata
     5041 cggctcccag gggcagcagc ggacggccgt cctggccttg aaattgtcgg agctggaata
     5101 cgtcaagtcc gagacgggtg aatatccggt cctgctcctg gacgatgtca tgagcgagct
     5161 ggacccggac cgccggcagg ccctgctcga tttcgtccgc gaccgtatcc agacgttcat
     5221 tacgacgacg gaaccgggga tctttgccgg catggccggc tgtcatccca tttgcattga
     5281 acaagggagg gtcctgtctc atggaggaac gcagtcttga attaaaaaaa gccggtttag
     5341 ccataccgaa agtattggag aaaaaccggc tccttttgcc ttatcactta taccaggcca
     5401 aacgggactg gaaacagatt gctggcgaac agattgccaa atattcatat atattagatt
     5461 ttaaggataa acaggttatc attgccgtca tgaacccggt atatatgaat tatctgttta
     5521 tgtataaaaa tgaaatcata gaggaaatta attcttacgt agggcatcgg gccattgccg
     5581 atgtccgttt tgtcaaaaag gggaaaaaac cggtccggcc ggtctatgag accttgcagg
     5641 gcgaaaggga agatgtcttt cctaaagaga ctatcagcca ggtccgcctc gatgacgata
     5701 cggtagcccg tatccgccag gaaacggccc atctggcaga agggctgcgg gaaaaagtgg
     5761 tccagctccg tttcgcccag gccaagcgga agaaggctta ccagctggaa ggcttcgttt
     5821 cctgtccctg ttgcggccgc tggatggcgc ctggcgaaag gcagtgcctc ttttgccgca
     5881 gcgaggcccg gcatgccttg aagcggcaga tacgcgctta cctcgacgac atgccctggc
     5941 tgtcgtggga ggccgtggcc ggctatctga agacggacgt gacggcggct gcggaagaac
     6001 aggcgtacaa tgaagtccgc cgcaatctca tctataccta tattgaaaag gtatattacg
     6061 aatacgatac ggcagccgat gattttaccc tggccatgct catcacccgc cgcgtccccg
     6121 gcgatatccc gccgaaattc atcgagaacc tcgttgccaa atacagaaag aaggacgacc
     6181 atgtatctcc atctgagccg taatctctcc atccatgccg gcgatgtcgt agccatcgtc
     6241 aacctgtcag gccgtcaggg ccgctcggtc tgccagcatt gcggcctgcc tctcgtcgcc
     6301 gtcgacggga taccggaaaa ggcctggcgc tgcctggtcg tcacaggaaa gcaggtcttt
     6361 gccctgccgg tcacgggaga gactgtggtc cggcgctatc agaagtgcct gcacccggca
     6421 aggtatgtat tcaaaaatgt ttgattttac ggtataataa agtgttagat tgcctaaatg
     6481 aatcattaaa attgcaagaa acaggagttg agaatctcta tgtcgaaaga caaagctaga
     6541 tatggtgccg aacagattca agtattggaa ggactggaag ccgtccgcaa acggcctggg
     6601 atgtacatcg gcagtacatc gacacggggc cttcatcatc tggtctacga agtcgtcgat
     6661 aacagtatcg acgaagctct ggccggctat tgtacgcaca tcgaagtaac catccaggaa
     6721 gacaacagca tcactgtcgt cgataatggc cgcggtatcc ctgtagccat gcataagatc
     6781 ggcaagcctg ctgtcgaagt cgtcctgacg gtcctccatg caggcgggaa attcggcggc
     6841 gacggctatc ccatttccgg cggcctccac ggcgtcggca tttccgtcgt caacgccctc
     6901 agcgtctgga cgaaggtcca ggtcaaacgg gatggttatt tatgggacat ctcctttacc
     6961 cgcggcgtga ccgatcagcc gctccggaag gtacgccctc tggaagcagg agaagaaacg
     7021 gggacaaaag tttctttcaa accggaccat gaaatcttcc cggacacggt ctatgacttc
     7081 aataccttga agacgcgcct gcgcgaactg gctttcctca ataaaggcct gcacatcacc
     7141 ctgaccgata agcgcggtga aggcaagagc gaagtctact gttatgaagg cggcttgacg
     7201 tcgttcgtcg aattcctcaa cgaaagccat caggccgtca atccgacggt catcaacgtc
     7261 gacggcgaaa agaacaaggt catcatcgac attgccatgc agtataacga cagctacagt
     7321 gaaaatatcc tgactttcgt caacgatatc ttcactgaag aagggggcac gcatctgagc
     7381 ggcttccgtt ccggcctgac gcgtacgatc aacgattatg gccgcagtgc cggcatcatc
     7441 aaagagaacg aagacaactt gtccggcgaa gacgtccgcg aaggcttgac ggcggtcatc
     7501 agcgtcaaag tacgggaacc gcagttcgaa ggccagacca agaccaaact gggaaacagc
     7561 gaagtcaagg gcatcgtcga caatatcgtt tccgacgggc tgaaagaatt ctttgaagaa
     7621 catccatctg aagccaaggc catcatcaat aagaccattt ctgcgtcccg tgcccgccag
     7681 gccgcccgcc gcgcccgcga gctgacgcgc cgcaagaacg ccctggaaat cagcagcctg
     7741 cctgggaaac tggcggactg ctcggaaaag aatccggacc agacggaaat ctacctggtc
     7801 gaaggggatt cggcaggcgg cagcgccaag cagggccgtg accgccgttt ccaggctatc
     7861 ctgcccctgc gcggcaaaat catcaacgtc gaaaaggccc gcctcgacaa gatcctggcc
     7921 aatgatgaaa tccgttccat gatcactgcc ttcggcacgg gcatcggcca ggaattcgac
     7981 ttgagcaaac gccgctatga caagatcatc atcatgaccg atgccgacgt cgacggcgcc
     8041 cacatccgga cgctgctcct gaccttcttc taccgcttca tgaagcccct catcacagac
     8101 ggccacgtct atatcgccca gccgccgctc tatcaggtgc gcaagggcaa acaggtctgg
     8161 tacttctata cggacgaatc gatgcaggcc aagctcgatg aactgggccg tgacagcaac
     8221 aacgtcgtcg tccagcgcta taaaggcctg ggcgaaatga acccggaaca gctttgggaa
     8281 acgacgatgg acccggaaaa ccggaccatg ctcaaagtcg aattggaaga cgccgccgaa
     8341 gcggaccgca ttttctcggt cctcatgggc gacaaagtcg aaccgcggcg caaattcatt
     8401 gaagaaaatg ccaagaaagt ccgcaatctg gacttgtaag aggagggcct atgccggaca
     8461 tttcccgaaa gatagactta ttcactgatt ccgtcatcgc ccgcatgtcg aaagtggccg
     8521 aagcctgcgg cgccatcaat ctggccgaag gcttcccgga ctttccgccg ccccaggcca
     8581 tgcgcgaccg cctcagcgaa gtagctcagg ccggaccgca ccaatacagc atggataatg
     8641 gcgcagccaa tctgcgccag gccatggccg actgccgccg catgttcacg ggccagacca
     8701 tcgaccctga acgggaagtc gttgtcacct gcggcgggac ggaagccctc atggcggcca
     8761 tgatgaccct cgtcgaccct ggcgataaag tcatcctctt ttcgcctttt tatgaagcct
     8821 acagtacaga cgccctgctc tgcggggcag agcctgtcta cgtatcgctc cgcgaaccgg
     8881 actatacctt tgatgccgat gaattggaag cggctttcaa gcagcatccg aaagcgattt
     8941 tcctctgcaa tccgtccaat ccgtgcggca aggtattctc ccgggaagag ctgatgacca
     9001 ttgccaagct ggtcgaaaag tacgatgcct tcgtcgtcat cgatgaggtc tacgagcaca
     9061 tcatctatga accgacacag tatacgtatt tttctaccct gcccggcatg tatgaacgga
     9121 cgctctgctg cggttccctg tccaagacgt attccatcac gggctggcgc ctgggctata
     9181 ttttcgcctc gccggctctg acggatgcca tcaagaaact ccacgtctac ctgaccatca
     9241 gtgcgcccag cccgctgcag gaagcggccg tgacgggcct gcgcttcggc cgggactatt
     9301 acgatggcct gcgggccatg tatacgcgca agcgggattt cgtcatgaag cgcctcgatg
     9361 acatcggcat cggccataat gtgccgcaag gggccttcta tatgctcgtc gatatcggcg
     9421 gttatggtta tgatgacgac gaagcgttct gtcgggacct ggcagaaaaa gttggcgtcg
     9481 ccttagtgcc ggcttcgacg ttcttcatcg aaccggtcca tcacctggcg cggctgcaat
     9541 tcgccgtccg cgatgaaacc ctggaaaagg cgatggaacg gctcgaaaat atttctctat
     9601 tgaaaaaata agttgcctat ggcaaccagg gggagggatt tttgatgaaa aagtggcaga
     9661 ttctcttagg agcactttgt atcagcgtat cgagtctgac agcctttgct gccgatgagc
     9721 ccgccaaggc ggagccggca gcggctatcg tcctgaccga cccgacggtc aacgtcaaga
     9781 acctggacga tttttatatc ctcaaaggga gtacctatga taaagtgacg ggacttttgc
     9841 aaaatctgcc gtcccagacg acaggcctcg ttggcagcta ttatttccgc gtcgacaaga
     9901 atgtcgtcgg cgattacttt tatcacgtca aggcctatga tacggtatcc cacgtcctca
     9961 taggcaatta tttcgtcgcc aaggatgaaa gctgcgcctg gaaactgcag gccggcgacg
    10021 atgcggccct gatttatggc aatgcagaaa aactgatgga taaggtcgaa gtcgtcgtct
    10081 atccgaagaa gatccccctg ggcagctacg gcatcatccg cgtccatgta ccggggatgg
    10141 taccctatga cgtcaaagcg acgagtttga atacgaaagt cgccgatatt acggaaaaaa
    10201 tgaatattca tcccatgcaa gccggcaaga ccgacttggt catcgacatc aagatcggtg
    10261 acactgtgcg cactgtgacc aagtcgatta ccattgtcga tgaagccgac cgccatacgg
    10321 gtgaaccgtc ccatagcggc cctagtgtcg gcgtaggcat cggcatcggc tggggcggcg
    10381 gctggcacca ccacggcggt ggtatcggta ttggcgtcgg cccttggtgg taaccattta
    10441 aaaggagtgt gtatctattg tggagttttc cctaggaaaa caatttctcg tcatcatttt
    10501 gctgattttt ggcaacggca tcttttccct cttggaaatg gccatcgtca gctgtcatca
    10561 atcccggctg gaagccctgg ccgatgaagg cagcaagtca gccgctattg tcctgaaact
    10621 tcgtgaaaat ccgaataaga tgttttccat ggtccagttc ggcattaccc tggtcagcct
    10681 gctcaccggt gtttacggcg gaaccaccat ggccggccct ttgtcgaagt atgtagccca
    10741 aattcctggc ttggaagcct atgcctatac gatcagcctg acgggcatcg tcgccttcat
    10801 tacctacttg acgattatcc tcggtgaaat cgtgcccaag cgcatcgcca tcgacaaacc
    10861 ggaaaaaatc tcctgtctcc tggcccggcc gatgctctat ttttccattc tctgtacacc
    10921 ggtcgtatgg attttgtcct ggtcgacggc gatggttacg aaaatcatgg gcgtcaataa
    10981 accggaagtc ctgcccgtta cagaagatga aatcaagctg ctcctggaac agggcgtcga
    11041 actcggggcc tttgaaaaag aggaaccgga actggtagat cgcgtcttcc gcctggccga
    11101 catggacgtc ggtgatatca tgaccaaccg gacccaggtc gattggattg atgtcgaaga
    11161 tccggaagaa acaatcatgc aggaactggt agcctttaac cacaacactc tgcccgtcgg
    11221 ccggggctcg ctggatgatt tcatcggcat gatttccgtc agcgaaatct tccagaaata
    11281 ctatgccgcc gtccggaagg ataagaccgt ttccctgacg accatcgtcg aagagagcgt
    11341 ccacaagccg atttatattc ccgaatcgat ggacattatg aaagtcgtcc acgtctttcg
    11401 cgaacacagc gtccatgaag tcgccgtcct cgacgaatac ggcaatttca gcggtattct
    11461 gaccctgcat gatatcctgg aagaactggt cggcatcatg ccggctggcg aagaagagaa
    11521 aaaggaggaa gcgaaccgca tcattcagcg cggccctaat gaatggctca tggaaggcct
    11581 gctgaccatc gaagagttca aggatttctt cgacatcgaa gacgatttgc ccgacgaaga
    11641 cgaagatttg tataagaccc tgggcggcct catcacctat ggtgtcggcc gtatcccgcg
    11701 ggaaaccgat acgtacgagt gggaaaattt cacttttgaa gtcatggata tggacaacct
    11761 gcgggtcgat aagatactgg tgacccgcag agatacaccg gatgccgatg aaaaagcgga
    11821 taaaggtgcc gataaatgat taggtctttg agaaaggaag tttgtatgga aatgggagaa
    11881 cgccaggcaa tttaccaggt gtctacgctg caggccctgg cccgcggcaa ttattacggc
    11941 agtaccgata tggaaacgct gctgacccat ggcgacacgg ggctggggac gtttcacgcc
    12001 gtaggcggtg aactcatcgt catcgacggc catgcttacc gcgtcttagg cgatggatcg
    12061 gctgtggaag cggccacgag cgatacgacg ccctttgcca cggtgacgtg gctgcgggaa
    12121 caggttcccg tcatattgaa gcgccagccg tcgattgaag ccctgaagga tatcctgacc
    12181 gagcaggtca gggaactggg catcaatgat gtctacatcg tccgcatcga cggccatttc
    12241 cgcagcgtat cggcccggac cgagctggaa cagcaggagc cctatctgcc ctttgccaag
    12301 gtcctggaaa aagatgaacg ccgcttcact tttgaagata tcgacggcag cctcatcggc
    12361 atctacatcc cggactatct gagcggcgtc aatacgtcgg gctggcattc ccacttcatt
    12421 tcggcagacc ggaaaaaagg cggacacgtc tttgacctgg acctggaaga aggccgtgtc
    12481 attttcaaca aagccgaccg cttcattctc gacatgcccc ataataaaca gttcgaacgg
    12541 ggcgacctga cggctgcctc gcaggaagaa atcaaacaga tcgaacaagg gaaatagttt
    12601 gtagtacgga gtttgtagtt tggagaagat ggctttaaat ttaaaaccat ccgctaaccc
    12661 ctagccacta gctgctaacc actaaaaaag gggctgtaac ataatagcct catcgaaaaa
    12721 aatggactca gttatc
//
LOCUS       DAWCYS010000027        12539 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_333_length_12539_cov_6.475409, whole genome shotgun sequence.
ACCESSION   DAWCYS010000027 DAWCYS010000000
VERSION     DAWCYS010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_12539_cov_6.475409"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2768 a         3489 c         3483 g         2799 t
ORIGIN      
        1 aactacaaac ttaaaactca aaaagaggct gtcttaatgc gacagcctct ttttgtcatt
       61 tccccggatc agccatgatt ttggccagga cgacgcggta tttatcgttg tattttcgca
      121 tgcagtcgtt gatgtcgcgg cggatgcggg cgatgttttc gacgttatat tctgccggcg
      181 ggatgtggat gtcacagatg acattgagcc gggcatggcc tgaggtgatg cgcaggtggt
      241 ccgtatgata acggggatat ttttcgtgga cgatcttatc caggacgtgg cggacgtcgt
      301 tgattttgtg gatgtctgtg cagagcgggt ccatgtggat attgacttcc atatggtact
      361 tgtcctgcaa ttcgtcttcc aggacgtcta aggcggcgtg gacttcgacg aggtcagcct
      421 tgtccgggac ttcggcgtga atcgagccga acatgcggcc cgggccgtag tcgtggatgc
      481 ggatgtcgtg gacgccgacg acgtggggac aattcaaagc agtcgctacg atgccccgga
      541 tgagttccgg gtccggcgcc ttgcccagga gaatgttaat gacgtctttg gcaatgccgt
      601 agccggcgta catgatcatc aggccgatgg ccgtgccggc agcggcgtcg atgggcagcg
      661 tcgtgaactg ctgggcaatg gtagcgacca ggacgcccgt cgtggcgatg gcatcggcaa
      721 tgctgtcggc agccgtcgca tcgttgacgc cggaattgat tttcttgccg atatagcggt
      781 tatagatgca catccagacc ttgacggcga tggagatgac gaggacgccg atgctccaat
      841 aggagaattc catgtcttct ggttcaaaga atttgccaat cgacgtttcg cagagcttgt
      901 agccgacgac gaggatgatg atggaaatgg tcagcgatgc caggtattca aagcggccgt
      961 ggccgaaggg atgttccttg tctggcggtc ggttgctcaa tttggcactg atgatggaaa
     1021 tgaatgacga acacatgtcg gtcaggttgt tgaaggcatc ggacaagata ccgatgctgt
     1081 tgctcatcat gccgacgagg aatttgctga caaagaggaa gaggttacaa ataatgccta
     1141 agatgccgcc gaggatactg tattgttcac ggacgtgttt gtcatcggta tcctcatagt
     1201 tcttgatgaa gcggcggata atgaattcga tcatatgaaa tcacccagct ttctgattcc
     1261 cttcattata gcagagatag ggcgagatac aagtaataac gagaaagaaa ttgagaataa
     1321 aagaaatgca aaaaaatcat aatattatcg aaaatgggaa tagttattga gcattttgcc
     1381 gggaagaatt gcaattactt tattttttta tataatagaa tagtgattga agaataataa
     1441 gctcataaag tatatgatgg ggaggatgta tggatatacg aaaagcactt atttttgata
     1501 tggatgggac catgtttgat acggaaccca tttcttatcg ctgttggcgg gacgtatcag
     1561 cccggtatgg ttttgacctg gaccgctcgg tgttcgaccg catgctggga cgggacaatc
     1621 tgcgcatccg tgctatttgt gatgacgcct tcggtccgga ttatccctat gacgatattt
     1681 gtcgggaaaa ggtggccttg cagctggaat attaccgcag ccatgctatt cccatcaagc
     1741 cgggcctgct ccacgtcctg cactacgccc gggaagaagg gattgcctgc gccgtcgctt
     1801 cgtcatcacc gcggacactg attgaatatt tattagataa aacgggtgta gctcccttct
     1861 tttccgtcgt ccagagcggg gaagaagcgg cccatggcaa gccggatccg gatgtgttcc
     1921 tcatggcctg cgacaaagcc cgtgtttcac cggaccaggc attagtattg gaagattcag
     1981 agagtggcat cctggccgcc catgctgcgg gaatcccgtc tatctggatc cccgatttaa
     2041 ttactattcc caaagacgta caagacctgg cctggacatg ttgccacagc ctggaagaag
     2101 ttccaggact gctggcgaaa ggggattcat aatatgacac agacaaagcg aagccgctgg
     2161 cttttgctca tgaccgtatt tttagggatg ctggcagcta ttgcaccgct gtcgacggat
     2221 atgtatctgc cagccctgcc gtcgatgatg acggcctttg gcgtgacgcc gtctgtcatc
     2281 cagctgactt tgacggcctc tatgaccggc atggcgctgg ggcagatcgc cgtcggccct
     2341 atcagtgatg ataaggggcg ccgcctgccc ttgatggtcg gcatggcgat ctttaccttg
     2401 tcgacggtag gctgtatcct gtcttcatct attgccgtct ttcttttgtt ccgcttcatc
     2461 cagggctgtg ccggcggcgc cggtatcgtc ctgtcccggg ccattgcccg cgatatctgc
     2521 aagggcagcg ccttgacgcg gctctttgcc atgctcatgc tggtcaatgg gattgcaccg
     2581 attctggctc ccgtcatcgg cggccagatc ctgcgctttg ccccgtggga aggcgtcttc
     2641 ctctttattg ccattgtcgg gctgatcctg accgtatcgg ccggtttgat gcgtgaaacc
     2701 ctgccgcagc ggcgccgcgt atccggcggc atggcagcca gtatgaagag ctttacggcc
     2761 ctgttccgtc agccttattt tatggggcat tgtatcatgc agtgcttttc ctttgccgcc
     2821 ttttttgcct atatttccgg atcatccttt gttttccaga acgtctacgg cgtatcagct
     2881 caggctttca gcctcatctt tggcatcaat ggtgtcggcc tcatgatcaa cggtgccgtt
     2941 acgggccggt tgacaggccg cattgctgac tggaaactcc ttcggttttc cctgtgtctg
     3001 gccgctgtcg gcagcgtggc cctgctggca gccttcagta ccggcatgcc gctgccggtc
     3061 atcatggtca ttctcttctt gaccgtatcg acgctgtcca tgatgagtac cagcagtttc
     3121 tccatggcca tgcaggacca gggacgcagc gccggcagtg cgtcggccct catcggcttc
     3181 ttctccatga tcagcggcgc cgtcatggcc ccagtcgtcg gcatcgccgg agaccatacg
     3241 gccatcccca tgggcatcgt catggtcatc ggcgaaatcg gcgccctcct ggccttctat
     3301 gccttgatct atccgcgaca tagaaaagaa cattgatcct ttgtaggggc tcgcacgtgg
     3361 cgagcccgcc ggggatatgc gagatacttg taaacttgat ttgtgtgagc gggcgtccca
     3421 cgtggacgcc cctacagaaa aaggagctgt cgtattacga cagctccttt ttttcatata
     3481 ttccgtgcga cgagtttttc gagtggttcg tctggccgct ggaaggggat atccggtttt
     3541 tctgccaggt aatccagggc aaagcccagg caggtggcag cgccgtaggt caggccgtct
     3601 tcgtcgaggt cgaattccgg cgtgtggtga ttggcaccgc atcctttttc tttgttttcg
     3661 atgcccgtaa aggtcaggac gccgggatag aggcgggacg tgatggcaaa agtttccgaa
     3721 gccatccagg gtgtgcagtc cgtcagggcg tcggcgccta tgtacttttc gacggctttt
     3781 tcggcgatgg ccacgcagac cgggttgttc tggacttcaa agagcggctc cggcatgtgc
     3841 aggatgtcgt aacggcagtc ataggtctgg caggcattgg ccaggatgcg cttgaattct
     3901 tcgtagaagc gcctgcctgc atggtcgtag ctgaagaagc ggcacgtacc ggcaaaggtc
     3961 aggctgttgg ggatgacgtt gaggacgtcg ccactgtgca ggctgccgat ggagaaggtc
     4021 agacattctt tcggcgggac ggcccgcatc cgcagggcct gcatgttgcc gtagaagtcg
     4081 tggaaacagt cgatagggct ttcagccagg tccggccggg agccgtggcc gccgtggccg
     4141 tcgatgcgga tttcaaagcc gaagccgccg gccatgggcg cgtcgtggca gacagagact
     4201 ttgcctgccg ggatgtccca gcggacgtgg gtcgcatagc aggcgtcgat atgccagggg
     4261 ctttctgttt ccaaatagcg caggagatat tccagagggc ccgattcttc ttcgccccgt
     4321 tcaaacatca agacgacggt tccgtcccac tggtctttcc agcgctggag gcattttgca
     4381 gccatgagct gcatggccat atggccgtcg tgaccgcagg cgtgcatgac gccgtgattc
     4441 tgagagcggc agacgcgcgg gcacgataaa ttccgttcgc cttcttcaat gggcagggca
     4501 tcgacgtcag cccgtaagag cagcgtcttg ccagggcctt tgccgtcaat ccaggcgatg
     4561 aggccgccct tgtcgaccag gcggtaagaa atgccatatt cctccagctt tttggcaata
     4621 taggccaggg tctggtcttc ctttttggac agttccggat gttcgtggaa atggcggcgc
     4681 aaggcgacga gttcaccttg ttctttgcgt acttcttcga gaatattgat catctttgct
     4741 tccccctctg tgttattctt caatccctat gcgggcggca aggccccggt caaagggatg
     4801 cttgattttc ttgatttctg agacgtaatc ggccaagtcc aggagggccg gactggggtc
     4861 gcggccagtc aggacgactt ccgtatgggc cggtttgtcc tttaagaaat cgaggacctg
     4921 ctcttccggg acgaggccca gggcgcaggc gccgacgagt tcgtcgcaga cggccaggtc
     4981 gggatggtgc atttcgcaga agtcccggac tttttgcaac aatgccgtct gggcctcaca
     5041 ggtggcctgt ttttcggcgt cagtcatctg aaaggtgaat ttcaggatgg ctttgccccg
     5101 caggaccgtg acctgcggca gggccttgag agactccagc tcgcctgtcg gccggccctt
     5161 gaggaactgg gcgaaaagaa tcttgccgcc ccggccggca cagcgcagga ccaggcccag
     5221 cgaggccgtc gtcttgccct tgccgtcgcc gcaatatata tgtattaagc ctgtatccat
     5281 catatcacct ctcattaact gttagtcatt cactatactg tggcgggcgc cccacgtggg
     5341 cgcccctacg ggggttatta taccataatc aaaaaatggc aggtagtggt caggcccatc
     5401 cattcgtgat ataatataac atcagttaat tttttttaga aaggaaggcc gggaagatgg
     5461 aagaacgagc ccgacagcta ttgacagctt tagaggatgc aggctttgaa gcctatgtcg
     5521 tcggcggtgc cgtgcgcgac ctgcttatgg gaaaagaacc gcacgactat gatatcgcca
     5581 cctctgcccg gcctgatgat atccgggccg tcgccgccaa agaggactgg cacaccgtcg
     5641 agatccacgg cgaagccttt ggcatcgtcg tcgtcgtcat tgccggccag acctttgaag
     5701 tcgcgacttt ccgcggcgaa gcctatggcg aggacagcca ccggccggat acggtctggt
     5761 atgctgatac attgcgcgaa gatgtcctgc gccgtgattt caccgtcaac gccatggcca
     5821 tggattgccg cggcgatatt tacgactacg tcggcggtca gaaggatttg cgcaggaaac
     5881 ggctggtcac cgtcggcaat ccggtccgcc gcttccagga agatgccctg cgtctgtttc
     5941 gggcctgccg ctttgcggga cagctcgatt tcatggcctc gaaagatctc gtcaaagcca
     6001 tgccccaggc ctttggccgc gtccccggcc tatccctgga acgagtcgtc aacgaaatgg
     6061 accgcctcat ggtcacgccg gcggcgtacc ggggactgga catcctcgtc cgcaccggcc
     6121 tcggcgcctg ctcgtgccgc cagaaggtga acggctgtta tgaagctgtc cccatcctgc
     6181 cggaattgag tcatctgccc gacacgccgc agtcgaaacc gttccatctc ttcgatgcct
     6241 gggtacacac cctggcgacc gtcgccaaca cgccgccgga tctgaccatc cgatatgcgg
     6301 cgttgtttca cgatgtcgcc aaaggcctac ccggtatccg cggcgtccat aagggccgct
     6361 ataccgatta cggccacgat gccaagggcg cagaactggc cgaagccatc ctgctgcgct
     6421 ggcacaagaa accggccttt gcccagcgcg tggcctggct ggtcaagacg catatgaaat
     6481 tccatttctt tgccaatacg gccgaaggcg acgtcgagaa atggcttcgc catgaagccc
     6541 tcgatggccc tttccatcgg acagaagaac tagtcgaagc cgtcggccag gctacggctg
     6601 tggcctgcgg cgatatttta ggctgcggcc atgccgacgc cagcacagaa gggacggaat
     6661 ccttcggggc ctatatggcc atgctggctg aggccatgcc agtcagtacc aaggacctgc
     6721 actacgaccg acggattccc gatgcgtgcg gccgccgtac aggcgactgc ctgcggatcc
     6781 tcttgaagcg ggtccagaac caggacctgg aaaatgaggc tgatgtactg gccgatgcag
     6841 cccggcgctg gatgaagcgc catgaagggg aagggcatca tgaataaaaa tgaaaagaac
     6901 ctgacccatc gcatttgcag ccgcatggtc atttgcatgg ctattttggc ggctatcggc
     6961 tttggcaccg ttacccgctt gtttatagag caagtcatcc atcatgacaa aaacgtggcc
     7021 tctgcccagg aacagagtca gattgaacgg aacctgcaat cgccgcgggg catgatactc
     7081 gaccgcaatg gcaaggtcct ggccatcagc gagatgtcca agtcactcta tgccgacccg
     7141 accatgctca atgaagaccc ggccatggta gccgatttac tggcgcctta tctccgcatc
     7201 tcgaaggatg aaattgaacg gcgcctgaaa gaagatacgg ccttcgtctg gctcgaccgc
     7261 cagatggacc acgaaaaata tgaagccgtc gaatcgctca tcaaggaaga caagctgcac
     7321 ggcctggctt tccgcgatga aaaccgccgt ttctatccga acggttcgat ggctgcccag
     7381 gtcatcggtt ttgtcggtga aaatgaccac ggcctggaag gcctggaaat gatgctggac
     7441 gatgaaatcc gcggcagcaa acagactttc cgcctgcaga ccgataacca taacatccct
     7501 gtcttttcgt cagccctgga acggatcctg cccaacaagg aacggtccgt ctgcctgact
     7561 ttggacagta ccatccagta cgtcgccgaa aagggcctgg acggcatcat ggcccgcagt
     7621 catcctcaag gtgcatccat tatcgtcatg aatcccaaga ccggtgagat attggctatg
     7681 gccagccggc ctacgtatga tcccaatgat tacagtaagg gaaataaaga agcttacaag
     7741 aaccgggctg tcgtcaacgt ctacgaaccg gggtcgacct tcaagcccct catggccgct
     7801 gccgccctgg attcggggaa atggcacatc ggcgatgtct atcacgacac gggttctgtc
     7861 cacgtcgccg accggacgat ttacaactgg gaccacaagg gcatgggcga cgtgaccttg
     7921 cgggaaatca tcatgtattc catcaatacc ggcatggccc atgttgccgt gacgacaggc
     7981 ggcaagacgc tgaccgatta cgcccgccgc tttggcttcg ggacgattac gggcatcgaa
     8041 ttgtccggtg aacaagaggg catcctgttc gatcccgata agatgtccat cgtcgataca
     8101 gccacgatgg gcattggtca gagcgtcgcc gtcacgcccc ttcagatggt acaggccttc
     8161 agtgccatcg ccaatgaagg ccacatgatg aaacctttcc tggtcaagga aatcgataat
     8221 cctgacggtt ccatttataa aaagacagaa ccgacagaag taaggcagcc catcaatgcc
     8281 ggtacggctg aaacgattag caagttcatg ggcgaagaag tcgctatcgg cggcggccag
     8341 agcgccaaaa tcgaaggcta tcgctttggc ggcaagaccg gcacggccca gaagaagacc
     8401 gaagacagca cgggctacgc cgaaggccag tatgtcggct ccttcatcgg ctttggtccc
     8461 ttggaagacc cgcagttcct ggtcctcatc gtcgtcgatg acccgcaggg cgtctattac
     8521 ggcgcccagg tcgctgcgcc ggtcttcaag gaaatgatga ccgaaatcgt ccgcatgaaa
     8581 ggcatccgtc cgacggaagc gatggaccag aaaccgctgg gttccgtcgt catgacgccg
     8641 gctcatgcca ttccgcccgt ccatatttcc agtgatggcg tcctcatgcc gtctttcatc
     8701 ggctggagta cgagggaagt caacgactgg ctcaatgaag cgggcttagg ctttatcccc
     8761 aaggggatgg ggaaggccat ccagcaatcg ccgcaggcgg gcagttatgc accgcagggc
     8821 agtaatgtca ccgttatttt caaacggaat ccatagaata acagggggaa ctagttatgc
     8881 aattactcaa cggccgggaa gtggccgcat ttcatcgcga acgggtggaa cgccgcctgg
     8941 ccgatatcca ggaagaactc catatccagc cggaactggc catcatcctc gtcggggatg
     9001 atgcgccgtc ggccatgtat gccagatcca tgcagaagac ggcccgggcc gtcggcttga
     9061 aggcagaaat ctatcagaaa ccggattcca tcagtgaagt agagctttta tcgctcatcg
     9121 ataagctcaa cgaacaggct tctgtcttcg gcatcctgcc catgatgccc ttgccgcgcc
     9181 acttgaacag ccagcgcatc atcgactgca tcgacccgaa gaaagacgtc gatggcctga
     9241 ccgatgtcaa catcgcccat ttatatacgg gacgaccggg gttcgtgccc tgtacaccgc
     9301 gggcagtcat tgccatcctc gattattacc atatttctct gagtggcaag gatgtagcca
     9361 tcatcggccg cagcaacgtc gtcgggaaac cgctggctca gctctgcctg aaccgcaacg
     9421 ccacggtcac gcactgtcat acgcgcacga aagacctgaa agccgtctgc cgccgggcag
     9481 acctggtcat cgccgctgcc gggagagccg gcttagtgac aggcgatatg ataaaacccg
     9541 gtgctgtagt catcgacgtc ggcatcaacc gcgtcgatgg caagactgtc ggcgacgtcg
     9601 cttttgatga agcggccgcc gtggccgggg ccgtgacacc cgtgccgggc ggtgttggcg
     9661 ccgtgacgac gatgatggta ctggaaaacg tcgtctgcgg catcgcccgg acagaaacta
     9721 ttttataaaa gtgagggagt aatctatgca tatcttagat aatttcatga ttcattattt
     9781 acgtcgcttc gataaacact gctttacggt acaacttcac gacaagacct acatcattgg
     9841 cgaaggccag ccgttgttca acgttattat tcacaaagac attccgaaaa aagaattgct
     9901 ggcgtcgacg tcgctggcac tgggcgaagc gtacatgcgc aaagacgtcg aaatccaagg
     9961 cgatttgttc acggctcttc gctgcttctt atcccagacg agccagttct cgctggataa
    10021 atccttgttc aaacgcatcc tctatccgtc ggaatcgaaa tcggctcaga agaaacaggt
    10081 ttcgtcccac tacgatctgg ggaatgactt ctatgccaaa tggctcgatc cgtccatgag
    10141 ctattcctgc gcatacttca agaatgaaga cgacagcctc gaacaggcac agcataataa
    10201 agtccattac atcctggaaa aactctacct caaagaaggc atgaccctcc tcgatatcgg
    10261 ctgcggctgg ggctatctgc ttatcgaagc tgccaagaaa tatggcgtca aaggctatgg
    10321 ctgcacactc agtaaagaac agtggaaaaa aggccaggaa cgcattgaaa aactgggcct
    10381 ccaggacctg gtacagatcg acctcatcga ctaccgtgac cttgtcgccg aaggccgtca
    10441 gtacgaccgc atcgtcagcg tcggcatgct ggaacacgtc ggccgctcca attatccgct
    10501 gtacatggaa acagccagcc acctcttgaa ggacggcggc atgttcctcc tccattacat
    10561 cagcggccac gacgaaagca tcggcaaccc gtggatgcgc aaatacatct tcccgggcgg
    10621 aaccttgccg tcgctccgcg aaatcgtcag cctcgcttac gacgatgact tccaggtcat
    10681 cgacgtcgaa agcctgcgcc gccattacta taagaccctc atgtgctggt tcaataactt
    10741 ccagaacgtc cgcgatgaag tcatcgctgc ccgcggcgaa gaattcgccc gcatgtggga
    10801 cctctacctc tgcggctgcg ccgtatcctt ctacatcggc aacatcgaca tccaccagat
    10861 cctcatgacc aaaggcacca agaacgacct gcccatgacc cgttggtact aatgtagtag
    10921 gggacgccca aagggcgtcc cgcaggtcgc aggtcgcagg ccgcagttcg cagttcgcag
    10981 gccgcaggtc gccggtcgca gggacgtccc cgcggctccc ctgatagggg agctggccgc
    11041 cgaaggcggt ctgagaggtt gattttctat tcactacaaa ctgaaaattt aatactccca
    11101 aagagactgt cgcacatgcg gcagtctctt tttttacgaa ccacgagcca cgaaccactt
    11161 tcactctcct atatacgcgt aaaacgtata accacagctg ctttgatgat aaaatggcat
    11221 aaatagtatg cttgactttt atatctacaa cggctatact aaaagtacag aaagagttaa
    11281 acgttatacg aatccggatg aattatcttt gaatagtaga aaaggggagt gtctcttatg
    11341 attaaacaga tcgtgaaagc cttagacggc aacgaagcag ctgctgacgt tgcgtataat
    11401 tttactgaag tcgctacgat ttacccgatt actccgtcgt cgccgatggc agaacacgtc
    11461 gacgtctggt cggccaatgg ccgtaagaat ctctttggcc agcccgtcaa attagttgaa
    11521 atgcagtccg aagccggcgc tatcggtgcc gtccacggcg cttcggaaac gggcagcctc
    11581 tgttcgtcct ttacggcgtc gcagggcctg atgctgatga ttccggtcat gcaccgcctg
    11641 tcgggcgaat tgaaaccggt cgtcctgcac attgccgccc gtaccgttgg gactcacacc
    11701 atgtccatct ttggcgacca ttccgacgtc atgggctgtc gcaacacggg ctgggctatg
    11761 ctctgcagtg ccagcgtcca ggaatgtgcc gacctggcag ccgttgccca tctgtcgacc
    11821 atcaaaggcc gcgttccgtt catgcacttc tttgacggct tccgcacatc ccacgaaatc
    11881 cagaaaatca agatgctgcc ggctgaagaa ctgggcaaac tcatcgaccg cgatgctctc
    11941 tatcatttca aacacaccgg cctgaacccg gaacatccgg ttatgcgcag taccgttacc
    12001 aacccggata tgttcttcca gtcccgcgaa gccaataacc cctggtatca gcgcctgccc
    12061 tacattgtcc aggactacat ggacaagatc agcgaactga cgggccgtcc gtaccacctc
    12121 ttcgattact acggcgctcc tgacgcagaa cacgtcgtca tcgccatggg ttctgtcacc
    12181 ggtgccatcc aggaaactat cgacaagctc tgcaaagaag gcaagaaagt cggcttcatc
    12241 caggtccacc tctatcgtcc gttctcgctg gaacacttta tgaaagccat gcctgacacg
    12301 gtccgcacga ttaccgtcat ggaccgcacg aaagaaccgg gcgccctggg cgaaccgctc
    12361 tacgaagatg tctgcgcagc catgtcccac gtcaaacagg acgtcaccgt cctggcctgc
    12421 cgctatggcc tgtcgtcgaa agacgtaccg ccggcatcga tcaacgccgt cttcaccaac
    12481 atggattctc tccagccgaa gaaccacata cccctgggca tcatcgacga tgttaccca
//
LOCUS       DAWCYS010000028        11936 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_356_length_11936_cov_9.248296, whole genome shotgun sequence.
ACCESSION   DAWCYS010000028 DAWCYS010000000
VERSION     DAWCYS010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_356_length_11936_cov_9.248296"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2463 a         3020 c         3160 g         3293 t
ORIGIN      
        1 gtgtcttgtg ggcattgtcc ggggcgccac gtgggcgacc cgttaggaat tttgaggatc
       61 ctctcagcag gcgggccggg caatcgtaca cccccccccc aagcgggctc gccacgtgcg
      121 agcccctacg acctgataac cattacttca gcacttcttc cgagctggtc gggaggttag
      181 gcatatcggt gttgaaccat tttttgtaga ttttctggaa ttcgcccgat tctttcagtt
      241 tggccatggc gtcgttgact tgtttcaaca attccttgtt gtccttgttg atggcgaagg
      301 ccaggtattc cggtttggta tcggggatat tgatgacatc gaatttatct ttgccgtcct
      361 gggtcagaaa gtaatcggca accggtttgt cgagaatggc tgccggcgaa ccgccgacct
      421 ggagttccat gatggcttcg gagttattgt cgaactgttt gatcgtatag ccctgttctt
      481 cggcatattt agcgccagtc gtaccgatct ggacggcaat ctgtttgccc tgcaggtcgg
      541 ctacggaatg gacgccgctg tttttcggtg tcaagatgac cagtttcgat tcgtagaacg
      601 gagcggcaaa ggcgactttc tgggcccgtt ccttggtgac ggtcatgccc gtagcggcga
      661 tatccatgtc accctgctgg atgatgggga tgataccaga aaaagcgatg ttgttgaatt
      721 cgacatcacg gcccatttct ttggctacgg cgcggatcaa gtccatttca aagcccttat
      781 attccgtcga ttggtcgtta tccttgaatt caaaaggaac aaacgtcgca ttggtagcga
      841 cgcgaagcgg tttcttcgta tcgttggcag catctttctt gtcactgccg cagccggcca
      901 gcatcgaagc ggctaatacg cctgtaagca aagcagcacc taattttttc gtatccatac
      961 aatcccctcc agaaattgaa taaaaataaa tttatgctta ttattatacc atcttgtggg
     1021 aaaaagtaaa gggcttgtcg caagcgacaa gccctttatc gttgttcgtg attcatgatt
     1081 cgtaggggag accacgtggg gcgcccgctc ctggggatgt acgaatcttc cggctacggc
     1141 taatctgctg gagaaatcgg atatatcccg aagcgggtcg ccttgacagg acggccccta
     1201 cgagcagcaa gcagctgact gcttgctgct actccttcaa ctccggcatc ttgataggtg
     1261 tcttatcgat gaggcggacg acgaggaggg tgatgatgca gatgatccat tcaaaataaa
     1321 tgacgtgggg caggacgcgg atctgcgggc agagctgcca ggcaaagagg ccgatgatgc
     1381 caaccagggt cgtccagaag gccgtgttct tgcggcacag ggacgggcag aacatggtgc
     1441 aaaggaagac cagcgtgaag gccgtcgtca ggctcaaacc gatgagcatg actttgacga
     1501 tgtcgacggc gttgaaggcc agccacaacg taccggcgcc gatgataagg atgattaggc
     1561 ggttggcctt gacgtagctt gcttcggaaa tatccggttt gataaaacgt ttaaaaatgt
     1621 cctgagagat gagggtaccg gtaccgagga gaatcgtgca ggccgtcgat acatcggccg
     1681 cccagagggc agccagggtc atccctgacg agaacgggtc gagactcatg atgatctgcg
     1741 gcagagccat ggtcggattg atgcccggga actgggcttt ggcagccagg ccgaggatag
     1801 ccgacaagaa gccgaccggg aagatgagca ggccgccgag gatgaagccc ctgcgggctg
     1861 ccttggcgtt cttggcgccg caggcaatct ggaccggccc ctgagccgtg atggcctgag
     1921 tcgtcatgac gatgatccag cccaggagta cggcaaagga caggccgccc atgggcgaga
     1981 accagttaaa atccgctgcc ggcagggctg cattgatgcc gctgatgccg ccgtcgcgga
     2041 gaaggatgcg gacgagggaa tagaggatgc cgaggtaaat gacggtgacg ctgaggatat
     2101 tcgataaacc ggaagaccag aggccgccga tggccgtgat gccgacgaag acgacggcac
     2161 tggtgatcat gccggcgtgc atggagaaga tatccggcat gagggcgtgt aaaatcgaac
     2221 cgccggcgac gtactgcagg gacgtgatgc agatcatgat cagggacagg ccgatgacgc
     2281 tgatgatgcg ggctttttta tcgtaactcc gttcaaagag ttccggaatg gtcgtgcagt
     2341 tcagggaacg gtacttaccg gcagcgacaa aacccatgat gatggcgccg atagcccagg
     2401 ccgcattgta ccagccggca gccaggccga ttttcgtcgc gctttcagcg acgccgacgg
     2461 tcgaagccgc gccgacggcc atacccgtga tactgacggc gaccaggccc gtagaaaact
     2521 ggcgccctgc aaataaatac tctgtcgggt tctgactggc ccggcgtttg acatagaaag
     2581 aaatggcaaa taataaaatg atgtataaga tgacaataca caattgaatc gacattttct
     2641 ctccatcctc ctttgactta taaatttaaa aatttgcaac cttagttccg tctatgggaa
     2701 cataatatgc caataattaa cgttattata ggctaaaacg aagaaaaata caaggagaga
     2761 gaaaacgcag gccgcagttc gcaggggccg tccccaaggg cggcctgcgt tttcgtaggg
     2821 gcttgcacgt ggcaagcccg ttggacattt gaataattct cttatcagga gctgtaggat
     2881 tccccaatcg tcttgctaag aaagtcttcc acactcctaa cgggtcgccc acgggcgacc
     2941 cctacagcgt aaaaaagggc ttgtcgccgt gcgacaagcc ctttttttca atctatgctt
     3001 ttttctgtgc ggccatggcc tggagttctg gtttgaattc ttcgaccagt ttcttttcgg
     3061 ctttggcacc tttggagagg gcgttgtaga tgatttccgc catttcgtaa cgggtcatcg
     3121 tgcgttcgcc gtggtatttt ccgtcttcgt agccctggat gatgccgttg ccagcgagtt
     3181 tgctgacggc ttcataagcc cagtggtttt ccgggacatc cgggaagtct ttggacatat
     3241 tcggattgat gaccgaaagg agggcatcga ggcggctggt gagataatca atctgtttct
     3301 gctgggcttt ttcccgttct gtcaagccgt tcgtggcatt gcgtcttgcc ttattgccac
     3361 cctggccgat acggagggaa atgccgccgc catacatgtt ttcgcctgtt ccgaaggacg
     3421 acgaaacatt gaccatgacg ttttcattcg gccggtagaa ggcgccgata gccgtagccg
     3481 tttcgccttt gtaaccgccg acagcagctg caatgttcca cttggaatca gcatcgaatt
     3541 cgaggggatg caggttggcc aatgccgctg cgttagcccc gactttgtcg acgcggctgt
     3601 ccagcttgtt gaccttgttt tcaacgccgg tgacgcgttc attggtctga tagagctggc
     3661 tgccattgac ggcatccgtg ctgtcagcgg caatccggcc cggagcgacg ttgaccactt
     3721 tctgatcgtt ggcgttgatg ccgttggaat cgatgtaggt cttgtcgccg accttatagg
     3781 aagaagcggt catgctaccg gcaaccgtag cgttgccgcc gacactgaga tctttttcca
     3841 tggtgacaat atccttaaag gttgcctggc catcgaaggt agaggtgctg gtgacgtgga
     3901 gtgtatccgt cgtcgtgccg ccagtaacgg ttgcaccatt gttgacgacg agtttttctg
     3961 tttttgtcgt accttctaca gtcaggtctt tttcaaccgt agcgttagat tccatggtga
     4021 cgtcgccttt caggtcagac gtaccgttga ctgtcaggtt cttgccaacg tttgcattgc
     4081 cctgcaggtc cgaatcgcct ttgacagtca gtttctgatc aaccgttgca ttgccatgga
     4141 caccgaggtc cttcatgaca tcagcataat ccatcgtagc ctgacccgtt tcaatcgtgt
     4201 tgccagaaat ggtggtcttt gtgacggtcc cttctttaat ggccgtattg tggtccgtgt
     4261 tttcaaaggt tgttccactg tccgtcgtcg ttaccgtcgt tttacctacg gtattggtaa
     4321 tggattcgct ggccgtgttg gtaatgttct gagcagcatt ggcaatggtt ccgccagcag
     4381 cactcaggct gatgtctttg gcactctgac tgataccggt actggtgttg ccatccgtta
     4441 cggcactgct gaccatgcct gttgtcgaat cttttacgat gtcgatgagg gtggaaccgt
     4501 tgaccagggc atcttccgtc ttggtggtgg cgctgtactg ggtatcgctg gctttgatca
     4561 gctgttccga tttgtccacg gtgctgttgg tgttgttggc cttggtcccg tcagtgatgg
     4621 aggaggccag gctcatggca tcctggttca caacggtggt gttggtctgg ccggcaatgg
     4681 tgtcgatggt ggtcttctta tccccatcgg tgagctggga tttttcttct gctgcgcccc
     4741 ggagatagga agcggtcttg ccatcgacct ggctcatttc ttctttagtc tgatagcaca
     4801 actgggtgga agtgttggag ccgtactcgt tggaaacctt gctgacaatg gagctggaag
     4861 catccgtttc tacatcaata gccttgttgg tgatcttgtt ctttgcttcg ttggtgatgc
     4921 tttctgctgt caggctgctg tcgccggtca cggtcgtggt ctggtttccg cctaccttgg
     4981 tatccaggtc ggacttgtag tcttccgtga ccttgccggt gacagtggtc ttcaattctc
     5041 caccaacggt gttggtcata ttgccggttg ctgtgttcac caggtcttta gccgtattgg
     5101 tgatggttcc attagctgcg ctgttggtaa tattaccggt tgcagtattc aggatatctt
     5161 tggcattggt tgcgaccgtc ttatctgttg cattgtttgt gatgccgctt tcattcatca
     5221 cactctgaac tgccgaaccg tcgccaatct gggtggttac agattcactg gccgtattgg
     5281 taatggtttt tgcatcattg gtcatcgctt cagcagcttt gttggaaata gtcttcgctt
     5341 cattgctgat agtgccgttg gaagcaatgt tgctgatatt gccattctct gctttgttgg
     5401 taatgctgtc agaagctgtg tttgtcagtt ctttggcatt attggaaata gtgccatttg
     5461 tggccgtatt ggtaatatcc gtcgtagtct gggtggaaga agtcgtgttg gttccatcgg
     5521 tgacttcgct ggtgatgtta tctactttct gatccacttt ggagaatacc gtatcagacg
     5581 aattaatctg attgaggatt tcatcgacgt gctgttcgaa tgtggaagtc ttgccattgg
     5641 catcattggt aatgacatta ctggattccg ttgcattcat cttttccaca tttctgttga
     5701 ttccattgga agctgtttcg gtcttacctt tttccgtcaa agtagaagtg ctggtatagg
     5761 tttttccatc cgtggtatct actttgctat tgacagaagt cacattcccg ccatgaattt
     5821 cggtataatt gtttttatcc agtttgctgg agagcgtctt ggcattgaat tcaccctgga
     5881 cttccaaatc tttcacagtc gctttaccac ttacgttcag ggtacctgca tcaacttctc
     5941 cagtagtctt gatggtgcca gcccctgcat ccaggtctcc accggtcagg gtcaggttgt
     6001 gccctgtcat gcctttggct accgtaaggc tgtcaccgct cagtactccg ctggctgtaa
     6061 tattctggcc ggaaatgttc ccggtccctg ccacgatatc tccgccgctg gtattcagtt
     6121 tgccgccggc tgtcaggata tcgccgccta cggttttgat gttgccggat ccagtgttga
     6181 tttcgccgcc agaagtagct actttgcccc cattggtacg gacttccccg ccctgggtat
     6241 tcacgttgcc gccgccggtc tggatgaatc caccgttggt cttgatattg ccattgctga
     6301 tggtggccgt tccatcggtc agggtagcgc cctgtaccgt tccggtagaa gtcacattcc
     6361 cagaagcaac attgccatca gtatccaccg taaacttccc gttggcccct ttgatgctgc
     6421 cgtcagcatt catagccgcc tgggctgccg catagttatc cccgccagta tagacgcctt
     6481 tggcatccat cacactgctg ttggtaccaa ccttgatgcc attttcgctg agggtaatct
     6541 ggttttcttt gccttcattc ataaccaggg tagtcgccgc ttctttggaa gtgctgacag
     6601 tcccatcctt gttcaccgtg gtggtgttct tttgcagtac attgttgtca ctcaggctca
     6661 gtccgccatt gaccgcttct gccagtttgg tatcagctgc cacacggcct gccaattcat
     6721 tggcatcagc ctggctcagg gcgttgaacc catccaccat attctggttg acctgctgga
     6781 tgaccttatc ctgggctgca tctgcaccca gccgagcagc cgtttcgtct gccagacctt
     6841 tatccagcag cgccacgttt tcgccaatgg tattggcggc tctcacgata gccccatcct
     6901 gtacggcggc atcgtccttg ctcacatagt tatcagatgt ctgtttttcc agatattcct
     6961 tcacattggt gatccgggta tccagagccg tatcggcagc ttcccgggca gcagcttcag
     7021 cggcatctgc cttttccaga ttcgtcacag agtatttccg tttcagtgct tcttcattaa
     7081 tggcatcagc attggctttg acctgcgtat ccagcgcatt caggttctcg cccacagatt
     7141 taaaagcatc aacatatgcc ccatccgtcg cttgaatatt ttcacctatc gaaaggagtt
     7201 cattttgaac ttccttacta aatttttgaa tatctttctg caggctttct tgccctttat
     7261 ctatcccttc tttggtataa acttctactg ccgtagtaga accagtgcca accggttcca
     7321 ccattatggt ggcatccgct gcatagctaa accctccgac cgggaggagt atgagggccg
     7381 tgatggcggc cagggccagg cgtctggtct tatcgcctgt actggaggcc ttggtatggc
     7441 ctttggctaa ttccgatgct acgacgtagc aatttcttga tttactccag atgactttaa
     7501 atatcttatt catattttcg caccctttct gtataaagat ttgacaggga ccttaatttt
     7561 gcgtacaaaa ataaaaggta ccggaaatgc tgaaattttt gggattttta tgcgtctttt
     7621 tggaaaatga atttgaaata atcttcctgg aactgggcga tattttcgtt tggccagggg
     7681 gattttgtgc cccttcactt cgatgtactc actctttatg atgtgaattg cagactggtt
     7741 gacgatataa ccttttccgg gagagacgaa ctggccgggg acgatggatt caaaggtctg
     7801 cagcagggct gacagggatt ggcgcgtttc caacatggag ccgtcatagg tgtggacttg
     7861 ctggacatgg ccgttgcttt cgatgaagag caggttgttg acttcttcga cctggatgcc
     7921 gccgccgacg aggcgaaaga tgatcttttt gctgtgccgc tggcgtatcg tgtccatgac
     7981 ccggtccatg gcctggtcga attgtttttg tgtaaacggc ttggtcaggt aatgggcggc
     8041 ccggacgtcg aaggcttcga cggcgaattc gtcacttgtc gacaggaaga tgatttcggc
     8101 ttgacttttc tgttttctca tttcacgggc gatatcggtt cccagcaggc cgggcatgca
     8161 gatatccaac aagatgatgt cgaaatcttt cctttcttcc acggcgtcta caaagttcat
     8221 ggcgttgtta taggcgtata tctcgatatt cgaggctttg ttctggtaat aagtctgtac
     8281 ggctgttttc atgatttcca actggtcttg taaatcatcg cagagggcaa ttctcaacat
     8341 aggtacctcc tttctgttca cggtaaggga acaatcatcc ttttgcaagt gtattataac
     8401 tcgtgttgat gcttaaaaat tcctccgtga catgaaatgt actttttttg gcataaacgg
     8461 aataggccct gatttttttt atacaatatc aagtacattg ctattattct gtaattatcc
     8521 tatgaaattt aagttttgac tccaaacgtc actttatttt gccgaattcc gtataactgg
     8581 aaaatgcttg aggtctatta tgcttccgta tacaatttac tggcatatta atgtgactga
     8641 gaaacgccat tgcatcgcgt tcctaaatat ggtatgatac ggccgaggca aaagataaca
     8701 tcacgtatcg tgagttatac ttttttcgga tatttttcaa gatggttttt tcatctgctt
     8761 ggatctgctt tggaccgaga cagaaaggat ggctcatcat ttgttggtag tgctgaaata
     8821 cgtgggattt ttcacaattt cgcctgccag tatggacgtt tgtcgcttag acacctttcg
     8881 gtctgtgccg gaaggtgttt tttttgtttc ttttgcccca gattctcata gggaggtttt
     8941 tttattgaaa aagtgttgtg attttctggt aaacaacatg gccattttcg tcgtcctcat
     9001 cggtattgtc ggcgccgtat ggccccagac cttgcaatgg gtcggcccgt acgttggctg
     9061 gctgctgggg attgtcatgt tcggcatggg catgaccctg actgtacagg atttcaagac
     9121 cgtcttcaag cggccgtggg aagtcctcat cggcgtcgtc gctcagttcc tcatcatgcc
     9181 cttcgtggcc tgggccctgg tacgcctctt tgccctgccg ccggaactgg ccattggcgt
     9241 catcctcgtc gggacctgcc cgggcgggac ggcatcgaat gtcatctctt atctggccaa
     9301 aggcgacgtc gccctgtctg tctcgatgac catgtcgacg acgctcctgg cgcctatcgt
     9361 gacgccggcc ctgacctggt tcctggcagg cgcctggatc catgtttcct tcgtatccat
     9421 gatgatttcc attgcggaaa tggtcctggt accggtcctg ctcggtttgg cggctcatca
     9481 tttcttcgcc cgcggcgtcg aaaaagtcct gccggccatg cccgtcgtat cggtagcgac
     9541 tatcgtcatc ctcgtcggct gcgttgtatc cctcagtgca tcgcgtctct ttgacgtcgg
     9601 cctgctcatg gcggctatcg tcatcttgca caatgtcttt ggcctggccc ttggctatgg
     9661 catggccaaa ttgttccgcc tcgattcgcg taaatcccgg accgtagcca tcgaagtggg
     9721 catgcagaat tccggcatgg ctgcgtcttt ggccgtctta tatttcaacc cggcagcggc
     9781 cattcccggc gctattttca gcgtatggca caatatttcc gggtccatcg tggccaattt
     9841 cttctcgcgc cgcgatgaaa aagccgaaga aaaggctcgt gccctgtctg tcgataaatc
     9901 gagtgttcct actcattaat tcatatttca aaggagtctt acaatcatga aaattttccg
     9961 tactgtcaaa gaaatgcagg catttgctgg tgaacagaaa aaggctggta aggttatcgg
    10021 cctggtcccg actatgggtg cgctccacga aggtcatctg accctgatgc gggctgccaa
    10081 ggccaaatgc gacgtcgtca tcgcttcggt cttcgtcaat ccgacccagt tcggcccgaa
    10141 cgaagactat gatgcttatc cgcgtcgctt cgatgaagac tgccagaaat tggaaagcgt
    10201 cggcgtcgat gccgttttcc atccggaacc gtcggaaatg tatcctgaag gctactgcac
    10261 ctatgtcaat gtcgacggcg acattacgca taaactctgc ggggctcagc ggcctatcca
    10321 tttccgcggc gtcgctacgg tcgtcacgaa attgatgaac attacccgcg ccgatgaagc
    10381 cttcttcggt cagaaagacg cccagcaggt cgtcgtcgtc cgccgtttcg tcagcgacct
    10441 caatatcccg gtccacatca atatggtccc catcgtccgc gaagaaagcg gtctggcccg
    10501 gagctcgcgc aatgcttacc tttcgccgga agaaaaacag gcggccctgg ttctgtcccg
    10561 cagtctgcgc aaagccaaag ccgcttatga aggcggcgaa aaggatgtag aaacactgaa
    10621 atccatcgtg accaaggaaa tccagtcgga accgatggct tctatcgact acgtcgatct
    10681 cttctccttc ccggccttga aaccgattgc gacggtcgac caggaaagcc tgttggctat
    10741 cgccgtccgc atcggcaaga cgcgtctcat cgataacgtc atcttgggct aaggaaatct
    10801 tcacattatc ttcacatttc ctccacatct ttttcgcacc cctctggtat agtagaagga
    10861 taaacagagg ggtactacaa aggagggttt cgtgatgtag aaaaaactcg taggcggatt
    10921 ggctgcactc gtagtgattg ccggtttttc cgcctttgca gcgacaccag ctgcttcaga
    10981 tggttcatcg tcagggactt acggcccggg ctatacgtgt tatgacaacg gtaacgggta
    11041 ttgccgggga ccgcgtgcct gctatgatga ccaggataac tgctatactg gaaatggcta
    11101 ttgccaggga ccgcgccgcg gctgccgggc tgatggccag cgcggttgtc agggccgtca
    11161 ggattgccct gcctatagcg ctcaacagta agccgttcta atcaaagggg gaaaacaata
    11221 tggtaatgac tcactacatg gaactcttat cgctctatga accttggttt ttgattctct
    11281 tcatgctcgt accgatggta ctggccgaaa cgattttggc tgccggtgct ttttccttgc
    11341 tctacaagga cagccgcagt gaaaaatggg acagcctgag ccacgtctgc ggcctcatcc
    11401 tgggtgtctt cttcatcgta gctacagtat acatcgtcac atcctacgtg ccgactatcc
    11461 agtggcgcgg cccgattgat tacatttcta tctgggccta tgtcctgggc gtcatcccgg
    11521 ccgtcctcat cctcttgcag gaattgggca tcatctttaa gggcctcgat acaacggcaa
    11581 aaatcaagaa acacatcgtc ctcatgattc tcttcgtctt gttcacccat ttagccatgg
    11641 tcttcggcat ggccgacccg cagctcgcag gctacgtccc gccgaaacag aacatgcaga
    11701 tgcagatgaa cggcaatatg cctatggacc acagtcagat ggaccatagc cagatgaatc
    11761 acgaccagat gaacggtcaa atgaatggtc agatgaataa caataacggc cagatggacc
    11821 acagcaagat gtagtttcgt ggctagtggc tagtggttcg tggcttcgta ggggccgtcc
    11881 cagagggcgg cccgcctctc ctgttaggag aggtggcccg aagggccgga gaggtt
//
LOCUS       DAWCYS010000029        11376 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_375_length_11376_cov_7.909814, whole genome shotgun sequence.
ACCESSION   DAWCYS010000029 DAWCYS010000000
VERSION     DAWCYS010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11376)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_11376_cov_7.909814"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2646 a         3234 c         3052 g         2444 t
ORIGIN      
        1 gggttagtag gggcgcccac gtggggcgcc cgtttacgca agtcggtcac gggattctta
       61 cacatccccg actacaaact acaaactatt tcccccgcaa gagccgcatg gccatggtgg
      121 acatgatgac caggagggcc gtgactaagg cgaaggaggc ggtcaggctg aaggcccggc
      181 cgatgaagcc cagtacggcc ggtgccagga ggatgccggc gtagccgata gtgctgacgg
      241 cggcgacagc atcagctacg ggcatgtctt tctgtgtccc cagagaggaa taaaagattg
      301 gcacggtgtt ggcacagccg acgccgatga ggacgtacga tgcaaacagg aaggggccgc
      361 cgctgatgag caggatgccc aggaatccgg caaaggcgac gggcagggac agggccagca
      421 gccgccgcgg cccgaaatgc atggtcaggg aatcaccggg caggcgggcc aggaaggccg
      481 acgccgtgaa cagggtcagg cccaggccgg attgggaaat gtcgatgccc ttttccgtcg
      541 tcatgaagac gccgctccaa tcgttgatag aaccttctac caggaaggca atggtgcaga
      601 ccaggccgat gaggacgatt ttccccttgg gcaggaccag ggaagcgccc ttgccggatg
      661 cctgccgttc cccatggaga tggggcgcaa agaagcagac gcagaggccg atgaagacgg
      721 cgccggcaat catgacgatt ttccagtaaa agcccaggtg gagcagggaa gcaaaaaagg
      781 cagcgccggc gaaattcccc agggaccaca tggcctgcag gccggacatg aggcgcttgt
      841 ggactttccg ttcaatgaag ataccgttga tgttgacgac gacgtcgagc agccccaggg
      901 acgaaccgag gaggacggcc atgaccaagg ccgctgcata agccggcaca taacagagaa
      961 cgttcaggac gacggcaatg gccaggccgg cgatggtcaa agaacggcgg cagcccaggc
     1021 gcccggcaat ggaaccggcc agcatcatca tgatcagggc gcctaagccg acggccaata
     1081 agaggaaccc caggtggtcg tcctgaatct gcagctgctg cctcaggaag ggaatgagcg
     1141 atgcccacga cgtaaaaccg aaaccgccga ggaagtaaaa ggctttggca ctataaatct
     1201 gccaaaggat atctcgttta ttcatcaatt tctgtcacct cacactaaaa aaagccggac
     1261 aagaaaccac cctgtcctgc atctttatcg aaagacgcgt ttacgtacgc gcccttcctc
     1321 cgctgcagga ctgaagtgac atcttatccg gctttacact atatgagcat aagcctccga
     1381 tgagcggtat tattataaca taaaaagaaa tttccctcaa gaagcacaat atttcttgac
     1441 agattctctt ccaggtgata atatgtacac caacaaccat acattgtctt tcatcaagag
     1501 cagtcgaggg aatggcccga tgacgctgcg gcaaccccct atcgggatag gtgccaagtc
     1561 cagcggtgta taccggcaga tgaacgaatc gcaaattgag ccgtcatctg tatgacggct
     1621 tttctttttg accggcaagc ccttattatc cgttatccag aaaggaaatg attacatgaa
     1681 cgcaacagta aaaaaactct tcgccgccgt tctcatcacg acgacgtgcc tctttgccgc
     1741 cggctgcggt tcgtcttcca attccggtgc ttccggtgac aaaaaagaaa tcaagatggg
     1801 cgtcactgcc ggtcctcatg ccgaagtcat ggaagaagtc gctaaagaag cagctaaaca
     1861 gggcattact atcaaagtcg tcgaattcaa cgactacgtc cagccgaaca aggccctggc
     1921 cgaaggcgac ctggatatga actccatgca gcatcagccg tacatggaca acgtcgtcaa
     1981 gaaacaaggc ctgaagatca cctccatcgg caagaccatt atcctgccca tggccgttta
     2041 ctcccataaa ttcaaagaca tcaaagacgt cacggacggc gccaaagtca ccatcccgaa
     2101 cgacccgacc aacggtgccc gcgccctgct cctgctccag caggccggcc tgattaaatt
     2161 gaagaacggc aactccgtcg acgcttcggt aacagatatc accgaaaatc ccaagaacct
     2221 ccagttcgtc gaactcgacg ccgctcagat tccccgctcc ctcgacgata cggacctggc
     2281 ctgcgtcaac accaactacg ctatcccggc cggcctgaac ccgcagaaag attcaatcct
     2341 cgtcgaatcg aaggattcgc cgtatgccaa cgtcatggca gtccgtcagg gcgacgaaaa
     2401 caacgaaaca tacaagaaag tcttggcaat ctaccagtct gaaccgatca agaaattcat
     2461 tgaagaacac ttccagggaa ccatcctgcc ggcgttttag tggctagtgg ctagtggttc
     2521 gtaggggccg cccaaagggc ggcccgcctg aatcctctta aaagggcttg tcgcacgtgc
     2581 gataagccct ttacttatgg tataataatg gttgtcatca gattcagaaa ggagacactc
     2641 ttgtcaacta cccccaccta acgccttcgg cgttcgaggt gggagcttgt aggtcggaaa
     2701 agcataggtg ctaacggcat ccaagatgtc tccctatatc cgcctacatc gtcgggtgat
     2761 tgacaacacc cgctttacca agtagtttac tccaaggtag tcattactcg ttcatatcga
     2821 ggatggttgt cccctgaaat ccacatagtt ccgagaagct ggatattcat ggctccaata
     2881 cggtcgtcgt tggattcgta tccgcactgg caactgtaca gatgcttatg gtggtcgcgg
     2941 ttcgccttgc ggatagtccc gcatttcggg caacgctggg aggtgtactt cgcagacact
     3001 ttcaggactt ccgaacggtt ctcgtgagcc ttgtatgtca ggaactgttc caactggtaa
     3061 aaactccagc tccgcaaatc atagttctgt ttcgcggttc tggaaagatt gctttcctcg
     3121 aagctgacgc cagtaaggtc ctcgagtacg aaaagcgtat ccttgccgta tttctgtacg
     3181 agtgtcttag atagctgatg gttgacatca gacatccaac ggttctctcg cccagaaatg
     3241 gctttgagtt tacgcttcgc tgattttgtc cctttggcct ggagctgttt ccgtacttct
     3301 tggaatttat ggcgtttggt ggcaatcttt ttgccagaga cgaactccgt cttgccttgt
     3361 tcgtcatagc tgactgcaag gaaacgaagc ccacggtcga tgccgacgac atggcggacg
     3421 ttctccttct ggaaatcttc gaccgccttg gtaacaggga tatgcagata ccatctccct
     3481 ttcagttcga ccagtttggc agtaccgagt tgataggaac catcaaggta tccagcgaaa
     3541 tgttcgcctt cgaaggtaca tttggttctt ttcccgagtg tattgatgga caggacttgc
     3601 ccgtcatcaa cgaagctgta gtctcggttg cggactaagt cagcctgtgg ccggcgaaaa
     3661 aatacgggtt tccagagcca ttccaaagtc ttagggatgc gttgccagtt gccatcctca
     3721 tccttgtatc tataaggctt tcggaataac tgttgcttga ccgtcttgta tctggcaatc
     3781 gtcgtcttga tagaagactg ggctaattga gacttcaagc cgaacaagcc acgaaggcca
     3841 ctatacagtt tcttgttgag gctttggtaa gtcaagtcga actgattgtc gaagatgtat
     3901 tgagacacga agttgcaagc ctgacggtac tgctccgtca tttgacggaa caatacttcc
     3961 tgttcaggag taacatgtat gcgaagtttt atcgttttgg ttaggtcgga catattctca
     4021 cctgcctttc taaaatgtat attgcttcat caaaaatatt ataacattgt tttttgctga
     4081 aattctactt aataagaaag tgaggtgagg cgggcatttc tctccgacct aagaggtcgg
     4141 agtatcctgc ccgcatttag atgaaagccg tatttgtcga tttcgagatg aaccctatcg
     4201 gccgggacca gaaggaagcg cgccgcatct gcaaaggaga aatcatcgaa atcggtgctg
     4261 tcaaaataga cgaagacggg aatgaaatct cgtcgtataa ggaatatgtg ctgcccgaat
     4321 atacgacagc catgaatcag acctgtcagg aattgacagg tatcaccatg gaaatgctgg
     4381 ccgggaaacc ccatttccgc caggccttcg accatttcct gacctggtgc aacgaaggct
     4441 gccccaatgc ctacgaaatg ttcgcctgga gtgaaaatga ctggcgccag ctggactgcg
     4501 aactccgcct caaagaaatc gacgagcacg acgaccgcgt ccgctggatg ctcgaccact
     4561 ggcagaactt ccagcagatt tactgcaacc tcctgggcct ggataatgtc atttccctgg
     4621 acaaggctgt cagcaccctg ggagaaacct tcgacggcca gatgcacgac gccctctggg
     4681 atgcccgcaa cacgtccaaa ctctacgtct tatcgaagaa aaaagaagaa ttccgcgata
     4741 tcatgcagcc tatcatcgac gccaccaagc cggcagaacc gctgacgttc tccctggccg
     4801 acgccttccg cgctgcccgc aagtaaaggg agggaatccg gccatggaag atacgacact
     4861 ccggatggtt tatattttca cagacatcat cctgcccatg attgccggat atgtcgccaa
     4921 aaggcggagc tggctgacgc cggaccagtc gaactggctc atccgcttca acatcatcgt
     4981 catcatgacg tccctgaccc tgctcagctt ctgggtcctg cccatgcgga cggacttgct
     5041 cagcctgcct ttcttcgctt ttttcaacgg cctcctgccg ctggccgtcg tcctcttgct
     5101 gggacggcag cgcaaattct cgtccttcgt cgaccgaggc agttacctca tcgccgccat
     5161 cccggccaac acggggatgc ccggcggcct gtgcagctac attctctacg gggaaatgtc
     5221 ctatgcctac gtccagatca tcggcgtctt ccagaacctg ctcatgttct tcgtcctctt
     5281 ccccatgggc tactactacc agcacggcgg caaggaccgc aatttcttcg tctttttcaa
     5341 ggataactgg aaatcgatct tcatcaactg gaaccagttg tccgtcgtag ccctggtcat
     5401 cggcacggcc ttacacgccg ccggcatccc caggcccgct gcggggacga cgattttcca
     5461 ggccctgatc cacgtcagcg cctgggtagc cctcatcccc gtcggctacc tcatcgactt
     5521 ttcccacttc aaggactatg cccgccagac gctggacctc attcccatca agatggtcct
     5581 gacgccgctg gcatcgtatt tcctggcctt gtgcttcacg tcggacccgg tgctcctggg
     5641 gacgattgtc atcgtcatgg ctacgccctg tgccatcaac gccttgatta cggagcgcct
     5701 ttacgacttg aacgtcaatc tggccatggc gcctttcatt acgacacagc tcttatatat
     5761 acttattcta tatcccgctt tttacctgct cgtatcctgg ggatacctgc ctttcaaata
     5821 actttcgcga ggtgcactca tgactcatac ccgaatccgt tccctctgtc tggctttatg
     5881 cctgctcatc cttaccctga gcctgacggc ctgctatacg attaccctca aagaacgccg
     5941 cgtcgacggc atgggctcgg ccatttccct gaaactgccg ggaaatctcc agaagctcga
     6001 cttccccgtc cccgtagacc cgctgaccca gcggtacttc gagggcagcc gcctgtacgg
     6061 tgaaatggac ggcggcctgt acatcgccat ctataccaac agcatcgaca cgcagcagat
     6121 gtacgacgac ctgcacatca gccagggcca ggccatcagc cagcgcctca accagacggc
     6181 cgaagccgca gccggcgccg tattatcgaa aatcgacgcc cagaacgtct cggtcagcca
     6241 ggacacgacg acggtcagcg gccagcacgc cgtcgtccag acctttacct tcacgtatca
     6301 ggataaatcc atgaaggccc gcctcatcgg ctttgccgac ggcccggcga cgtggatcgt
     6361 cgtcgtagcc tgcgacggca gcaaagaaga taaggtaaag ctcgctgacg aagtactcaa
     6421 ctccatccga atcaattaat cgcctgattt tcctcttgca tgcgccggta aatgggcagc
     6481 cgcttcaagt ggcgcaaggt gaaaatagcc gtaatgccga tgaggatgcc cgttcccgtg
     6541 ccggcgacgg ccaggatcgg caggtagagc atgatcttga tattctctac gaccaaagag
     6601 gccacgatga cctggcccag gttgtggaaa ataccgccag ccgtgctgac accgatgata
     6661 gagaatttcc ccgtcttctg caggaccatc atggcgccca ggctgaaagc cgccccggca
     6721 acgctgtaca agaaagcgtt gatgccgccg ccaaaggccg tggccaggat gatgcgcacg
     6781 agcaggatga gacagacgtc gcgcttacgc ggcagggcat agagggcgat gaccgtaatc
     6841 agattggcca gccccagttt cgcccctggc gcgatgaagg gcacgggcag catcccttcg
     6901 acgacgtata agaccaggga ctgggcgatg aacaggccca ggataataat gtgaaatgtc
     6961 tttttcataa gtacctctaa tgggccggaa tgacatcggg ttcgtcatcg ccggcggact
     7021 tcacttcgac cagcaccttg tgcggcaggc agacgacggt cgcaccgggc ttgctgacaa
     7081 agccttcact gatacagatt ttatcgggac aatcggcttc ggtaatgccg acgctctcgt
     7141 cctggatgac cacgaggtta tagccctcct cggtctggat agggatgaca tcggtcccat
     7201 ggtggcccga caagggaact tctttataga ccttgccgtc gacctggatg accgcatacg
     7261 tccggctcac agacgcatcg ttaccggtca ggtaaaagat gccttccggg atgaaagata
     7321 agacgagcag cgtaatgatg aggacgatgt cccctttttt gatgatatgt ttcataggat
     7381 ttccatattg acgaacagga gaatgaacat gaagaacaaa cacttggcca tcctgctcct
     7441 gctggccctg gctgccggga gcggcggatg cagtacacag ctatcccagc cccagccggc
     7501 tcagacggca caggacgtac aggctccatc ggcaccggcc atccaggccg attcgcaggc
     7561 ttacagcgac gaaggcctga aaaaatacca ggccggcgat tatgccggag ccctgaacga
     7621 tttcgacaag gccctttccc tcgacgccaa caactatcag gccttgagca ataagggcgt
     7681 caccctggcc atgcgcggca acagcacagg cagcaaaaac gacgtctccc agggcatcga
     7741 gctcatcgaa aaagcgctga aaatagcccc gaaagacgtc gcttctttct acaacctggc
     7801 cttagcctat aagattgacg gccaatacga caaggccctg acgtatttca agaacgtcat
     7861 cgccgccgac ccgggcaata cctggagtta ctacggcatc gccaccatct acggcgacct
     7921 gggcaaagcc gaccaggccc tgccctatct gaagcaggcc atcgacctgg gcggcgaaga
     7981 cgtccgccag gcagcccgga cgcagagcca tttcgataag atacggaaca acaaacagtt
     8041 ccagcaaatc gtcggctgat tacatgtctt cctcagcgat ttcgcagagg atgtggccaa
     8101 tcatgatatg catttcctgg gcccgtgccg tcactttggc cggtacggcc aggcagatat
     8161 cgcaggcctc cttcatcttg ccgccgccat aagcggtcag gccgatgaca gtgaggcctt
     8221 tttcacgggc tgcatcgatg gcttccagga cgttctggct gttgccggac gtagaaatgc
     8281 cgaccaggac gtcgcctttt tcgcctaagg cccggacctg gcgggcaaag accgtatcga
     8341 agccataatc attgccgatg gccgtcagga tcgacgtgtc gacggtcagg gcgatggccg
     8401 ggaacgggcg ccgttccttt tggaagcggc cgacgatttc cgcggctaaa tgctgggaat
     8461 cggcagcact gccgccgttg ccgcaaaaaa gtatcttgtg accagaagcc agggcctctt
     8521 tgaacagagc cccggctttt tcaatatccg ggatcaactc catcgtcgcc tcgaggacgg
     8581 cggcgtggtc tttcattcgt tgtgtgacca gactcataat acctcctgtt cctggccttc
     8641 tccatcgtcg acgacgcgga tttcaccggt gtgcggatgg atgaccatac cataaatcgt
     8701 aagggatgaa ggcaagtagg gattggcccg caggcggcgg accgtatcgc agacagacgc
     8761 gtcgatatcg gtaatggggt cggcccactg ttccaatttg gggcggacgg cggcgatggc
     8821 cttttcatcg atgccttctt cggccatatg gcggcacagg ctgtctgccg tcgtcttgag
     8881 catgccgcag tcgtcatggc ccatgacgat gatgtcgtgg acgtgcagtt cgtagacggc
     8941 aaccatgagg ctgccgatga cgctgtcaaa gtcttcaaaa gccgtattgc cggcgacttt
     9001 gatgagtttg gcttcgcccc gggcgatgcc catggccggt tcgaggaaat tcaagaggcg
     9061 cgtgtccata caggtcagga cggccaggtt gcggctcgga tacttgctga cttcatcgaa
     9121 gccgccctgg gacagggact gtttgacaaa agacttattg gctttcagta tctgccgtgt
     9181 caattcactc atagcggcct cctcatgctt tcacagcggc catgactttt ttggccttca
     9241 agtactggtc gataccttcg gcagtcttac gcccttcgcg catggccagg acgacggtct
     9301 gcggcttgtg gacgatatcg ccggcagcaa agacgccttc tttgctggtc aggccgtaag
     9361 gcatatcctt gacggcgatg tagccatcgt cattggcatc gacgccgaga gctttgacga
     9421 cggacaattc cggcttgttg ccgatggcgg cgatgatttt attggccggg acgacgccga
     9481 attctcccgt cggtaccatg gtggcatctt cgaggatctg ctgtttttcg tatttcaggc
     9541 cttctacatg gtccgtcccg acgacggctt tcggtgccga atagaactgg aactggacgc
     9601 catcggcctt ggcttcttcg tattccgacg gcaggcactt catgttcttt tcagcccggc
     9661 ggtagacgat tttgacgtcc gcaccgaggc gcagcgacgt gcgggccgca tcgatggcga
     9721 cgttgccggc gccgacgacg atgacctggt cgcctttctg tacgggcagg tctgctagtt
     9781 cgacttcacc ggtctggacg cgctggacgt cgcgcaggaa ctgttccgca tcgacgacgc
     9841 cgtcgatcgt gtcgttgtcg acgccgagat tccaggcatt catcgtaccg atggcgatga
     9901 agacggcatc gaaatcacgc tgcagttcgt ccagggattt gtcgacgccg attttgacgt
     9961 tatattcgat ggtgacgccc atagctgtca gggcctgggc ttcgcgctgc agtaattcct
    10021 tatggagccg gaaagtcggg ataccatagg tcatggtacc gccgggctgg gaagcggctt
    10081 caaagatggt tacatcataa tcgcggccag ccagttctct cgcagctgcc atgccggccg
    10141 ggccgcagcc gatgatggcc acgcggcctt ctgttttacg gctgaccggc tgcggcagga
    10201 agccgccatc cagggccatg tccgctacga agcgttcgag tttgccgatt tcgacgtgtt
    10261 tgcctttttt gccgaggatg cagtggcctt cgcactgctt ttctctcgga cagatgcggc
    10321 cgcagatagc cggcaaatca ctgcggtgat agatatgttc agcagcggcg ccgaaatcgc
    10381 ccaaggtcat ggcatgtatg aactggggga tttcattttc aatggggcag cctttgcggc
    10441 actgcggtac tttgcaattc aggcagcgtt tggcttcggc aacggcttca gccagcgtat
    10501 aatcagcatc atgcatgact tcttcatgga aagaagccag ccggttcttg gtatcggaca
    10561 tgagagaacc cacctttccg gattgtaaaa tgattagatt gtattcaaga taaaaaaatg
    10621 gtgcgcccaa gatgactcga acatccgaca cgcagtttag gaaactgctg ctctatccag
    10681 ctgagctatg ggcgcacagg gcacacaggt ctgctgcgtg cagattaatc ataccatgaa
    10741 acggcaaaaa aataaaggtg ctggctcatg aaaaaaaggc ctattgcaag tctttcaaac
    10801 actctctata attagagatg tgtatattgt tcccaagcgg caatagccac gactgcatga
    10861 aagaaaggaa tcaagcatga tcaatttaga aaccctggct acggaacagc agaacagcca
    10921 tagccagcat atcgacagcg tatcgaccga agatatgctg cgcatcataa atgaagaaga
    10981 ccataaagtc gccgatgccg tcggcgccat catcccggcc atcgcccaag ccgtcgacac
    11041 cattgccgat aaactgcgcc gcggcggccg tctgttctac atgggctcgg gcacgtccgg
    11101 ccgcctgggc atcctcgacg ccgtcgaatg tccgccgacg tacagcaccg acccggaacg
    11161 catccagggc ctcatcgccg gcggttatga agccatcttc cgggccaaag aaggggctga
    11221 agactcgccg gaactgggcc ggcaggattt gatagacaaa aacctgacgg ctgacgacgt
    11281 cgtcgtcggc ctcagcgcct cgggccggac gccgtatgtg ggcggcggcc tgacctatgc
    11341 ggcctcggtc ggagccgcta ccatcgccat cgactg
//
LOCUS       DAWCYS010000030        11085 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_384_length_11085_cov_6.702629, whole genome shotgun sequence.
ACCESSION   DAWCYS010000030 DAWCYS010000000
VERSION     DAWCYS010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_11085_cov_6.702629"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2239 a         3432 c         3164 g         2250 t
ORIGIN      
        1 attaagtaga ccagtccgac gaagtggcgg aacagggatt aggctgccag gcagaaggac
       61 atgcgcatgc cgaaggcttc ggccaggatg ccgccgaaga gggggccgat gatgccgcct
      121 gccgtcaggg cgccctgcat gatgcccagg cagatgccca gtttcttcgg cggtgcatag
      181 atggtcatga tggccagttc catcggccac aggcccgatg cgaagccctg gaacatgcgc
      241 atgaacgtca gctgcagcgg cgacgtgacg atgccgccga gaaaatagct gatcgataag
      301 agcaggctgg cgcggatggc catgagccgc ttgccccgcc ggtcggccat gcgtccccag
      361 ataggcgcca tgatggcgct gatgaggaag gtcgacgaaa agacgacacc ggaccagatg
      421 ttgaccgagg ccgcatcgac gcccagttcc cgcgtcaggt acatgggcag gaaggggatg
      481 agcatggtat agcttgaaga catgaagacg acgttgcagg tcagaatggc cagacagaat
      541 ttccagttca cggcgatcac ctttctcgta tggattgtta acgtactaac ttcttattat
      601 acgccgcctt ttcggggatt acaacttgtc acgatgggaa cacagcctta taatgaataa
      661 tatctatgtt ggaaaggaag gaacctgaat tggccatgta ccttgcaaag cgcctggccg
      721 gcgcttgtgt cgtcttatgg gcgatcatca ccattacttt tggcctcatg catgccatcc
      781 caggcggccc gtttacgcag gaaaagaaac tgccgccggc cgtcatggca accgtcgaag
      841 cccgatatca cctcgacgaa cccttatggt cccagtacgt cgattacgtc cgccatgcag
      901 cggtcctcga cttggggcct tcttataagt atcccggcaa gaccgtcaac gctatcattg
      961 ccgccacctt tcccgtatcg gcccagctgg ggctcatcag cctctttttg gccatcggtg
     1021 ccggcgtcct ggccggcatg gccgcggcct ggtataagaa tacctggatc gactacacca
     1081 tgatggtcgg cgccaccctg ggcgtctccg tgcccagttt catcctggca gcggtcctca
     1141 tccagctctt cgcctttacc tggcccatcc tgccggcggc cctgtggaag gggccggctt
     1201 acgttgtcct gccggccctg gccctggcgg cccagccgac ggccttcatc atgcgcctga
     1261 cccggtcgag cctgctcgat gccctggggc aggactatat ccgcacggtc cggtcgcggg
     1321 gcatcggcac gcggtccctc atctgccacc atgccctgcg caacgccctg ctgcctgtcg
     1381 tcagttatat cggccctttg gcggcggccc tcatgacggg gagcttcatc gtcgagacga
     1441 tttttgccat ccccggcctg ggacgccact tcgtcaccag catttataac cgcgactata
     1501 cggtcatctt gggcattacg attttttaca gtttcctcat catgatgatg aatttactca
     1561 tcgatgtcat ttatccgctc ttggacccgc ggatcaccat ggatggaaag aaggagggct
     1621 gatgatggat tttacaccgc ttacggctgc tgaccggacg gaaccctatg taccgcccca
     1681 gaccctgcgc gaccggggct ggcggcagat gaagaagaac cgcctggcct tatggggcct
     1741 ggccatcgtc gtcgtcatga gcctcctggc cattgccggc ccctggctgg cgccctatac
     1801 ttatgccgac caggatttga cggcagccaa tgcctggccg tcggctgcgc actggttcgg
     1861 tacggattcc ctgggccgtg acctcttcgt ccgcgtcctc tacggcgccc gcatttccct
     1921 gtccatcggc ctcgtcgcca gcctgatcaa cgtcttcatc ggcgtcatct acggcggcat
     1981 tgccggcctc gtcggcggcc ggacggacca gatcatgatg catatcgtcg acatcctcta
     2041 ttccattccc atgctgctct acgtcatttt gctcatggtc gtcttcaagc cgggcctgct
     2101 caacatctac ctggccctgg gcattgccta ctggctcaac atggcccgca tcgtccgcgg
     2161 ccagatcctc agcctgaagc agcaggaata cgtcatggcc gcccgctcct gcgggacgtc
     2221 gacgtggcat atcctctgcc gtcatatgat tcccaactgc gtcgggccga ttatcgtcac
     2281 cctgaccttg tccattcccg acgcgatttt tacagaagcc ttcctgagtt tcatcggcct
     2341 cggcgtatcg gcgcccatgg ccagctgggg tgtcctggct tcagaaggta tcaatagtat
     2401 gcgctccttc ccgttccagc tcgtcttccc ggccctggcc ctgtgcatca ccatgctggg
     2461 cttcatgttc ctcggcgacg gcctgcgcga cgccctggac ccgcagaacc agaaggaggg
     2521 acgctgatgc aagccttatt agatgtacgc catctgacca tttctttcca tacttaccgg
     2581 ggactgctgg aagccgtcca cgacgtatcg ctgaccgtca atcccggcga aaccgtcggc
     2641 atcgtcggcg aatcgggctg cggtaaatcc gtcacgtccc aggcggccat gaagctgctg
     2701 ccggccgaag cgacggaata tactggcggc cagatcctct gggacggccg cgatgtcatc
     2761 cccttgtcgg aaacgcagat gaaccgcctg cgcggccgcg acatggccat gattttccag
     2821 gaccccatga cgtcgctcaa tcccgtcctg accatcggca accagctctg cgagggcatc
     2881 gccctgcacc agcacctggg ccgtaaagcc tgtgaagcca aggccctgga actgctcaga
     2941 gccgtcggac tgacgtcgcc ggaaaagcgg ctccatcaat atccccatga attatcgggc
     3001 ggcatgcgcc agcgctgtct catcgccatg gccctgtcct gttcgccgcg gctcctcttt
     3061 gccgacgaac cgacgacggc cctcgacgtg acgacagaag cccaggtcct ggatgtcctc
     3121 aagcgcctcc aggcggaatt gggcatggcc gtcgtcctca tttcccataa tctcggcgtc
     3181 atcgcccaga tgtgccgccg catttacgtc atgtatgccg gcgccatcgt cgaagagggg
     3241 acggccgatg atattttcta ccgcccggcc catccctata cccagggcct cctggcctca
     3301 ctgccggacc cggcccagaa ggataaagcc ctgaccggga tccccggcca ggcgccggac
     3361 ctcttccaca tgcccgaggg ctgccggttc tatccgcgct gcccccaggc catgaaggcc
     3421 tgcatgacag cggcgccgcc gcgggaagaa gccggaccgg gccatcatta cacgtgctgg
     3481 ctggcccagg ccgccgcggc caaggaggca ttgccatgaa cctcatcgaa gccaaacata
     3541 tcagtaaaca attcgtcgtc cagcgggact ggctgggacg gcccaagaag accctgaccg
     3601 ccgtcgacga cttgtccctg accatcggcc agggcgagac ggtcggcctc gtcggcgaat
     3661 cgggctgcgg caaatcgact tttgcccgga ccctgctggg cttatatgcc aagaccggcg
     3721 ggtccattac ctataagggc agggacattg ccgaccgcgc tgtaggccgc gactatcgcc
     3781 gccacgtcca gatgattttc caggaccctt atgcctcgct ggacccgcgc atgaccgtcg
     3841 aagacatcat cagcgagccc ttacgcaact accatctctg tcaggacgac agccagcggc
     3901 gccagcgcgt ggcgtcctta ttggaacagg tcgacatgcg gcctgaagcg gcccagcgct
     3961 atccccatga attcagcggc ggccagcgcc agcgcatcgg catcgcccgg gccctggccg
     4021 tcgacccgga atgtgtcttc tgcgatgaac ctatttcggc cctcgacgtg tccgtccagg
     4081 tccagattgt caacatgatg cagcgccagc agcaggaacg gggcctgtcc tatctgttca
     4141 tcgcccatga cctggccatg gtccatcaca tgagccgccg catgggcgtc atgtatttag
     4201 gccgcctcgt cgaattggga ccgggcgacg ccgtcttcca cgacccgctc cacccctata
     4261 cgcagatgct catcgcctcc gtgccccggc cggacccgca ctaccggcgc cggaccatcg
     4321 tctgcggcga agtgccgagc cccctcgact tgcccaaggg ctgcgtcttc catccccgct
     4381 gtccctacgc cgatgaagtc tgccgcatcg acgtgcctca tttccggcag gtcgcagacg
     4441 accgcttcgt cgcctgccat catgcgagag gaggcagacg atgaagaaat tactcttact
     4501 cctgagctgc ctgctgacgg ccgtcctctt cagcggctgc ggcattcagc accccgatac
     4561 ggtcagctac gtcctggaag cggaaccgtc gcggctggac ccggccatga cgacggccct
     4621 cgtcgaaagc aatgtggaac tgcagatatt tgaaggcctg acccgcctcg acgatgacga
     4681 tgtaccccag ccggccctgg ccgaaagctg ggatatctca ccggatggca agacgtatac
     4741 cttccacctg cggccgggca tcgaatggag cgacggcacg ccgattacgg cagacgacat
     4801 cgaatattcc tggaagcgcg tcgtcgatcc cgacgtagct tcggaaaatg cctatatgct
     4861 cttttgcatc gacaaggccg aagactattt caataagaag gcctcggccg atgccgtcgg
     4921 cgtcaaagcc gtcgatgacc ggaccctggt cgtccgcctg aaggaaccga caccgtattt
     4981 cctcaacctg acggcgttcc actgctatta tcccgtcccc aggaagctcg tcgaagccaa
     5041 gcccgatacg tgggctgccg atgcggacgg catggtctgc agcggcccct ataaaatcgt
     5101 ttcctggaac cattccagtg aaatcgaagt cgtcaagaac gaccgctact gggatgccgg
     5161 ttcggtcatc ctggaccacc tcgatttccc catcagcgat tcccaggcga cgcgcctgac
     5221 cctggtcgaa agcaatcagg ccaacatgac cgtcgaaccg ccgccggccg accaggaacg
     5281 gctggaacag atgggcctct acgagattgc tccttatctc ggcgcctatt actacgtctt
     5341 caacgtccag aagaagccct ttgacgacgt ccgcgtccgc aaggcctttg ccctggccgt
     5401 ccagcgccag ggactgatga agtacatcgt ccgcggtgaa aaggaagcgg cctacgcctg
     5461 ggtcccgccg ggactgaagg acaaggccac aggccgtgat ttccgtaagg aagggggtaa
     5521 cctcatcgcc gaagacccgg ccaaagcccg ccagctcctg aaggaagccg gttacgatga
     5581 ccagcacccg ctgccggaag tgacgctcct cttcaatacc aatgaaatgc acaaggctgt
     5641 agccgaagcc ctgcaggcca tgtggaaaga aaacctcggc gtcgacgtca atattaccaa
     5701 ccaggaatcg aaagtcttca tggccacccg cgcccaaggc gactaccagc tggcccgggc
     5761 gtcgtggatc gccgattaca tcgaccccat gaccttcctc gacgtctttg ccgatgacga
     5821 aaatgacgcc cagtaccaca acccggcgta taatgcattg attgccaaag ccaaggccag
     5881 caacgacgaa agacagcgca tggcctatct ccacgaagcg gaacagatac tcttcgacga
     5941 ctgcgtcatc atccccttgt actacacgac ccagccctac gtggcccagc cctatatcaa
     6001 gggctaccac tggtcgccgc tgggactggt cgatttcaag aaagcctata tcgatgaatc
     6061 agaaaggaag tagccttatg gagtatacct atgcagaacc gttgcaagct ggcgacacca
     6121 tcgccgtcgt cgccccgtcg tcagccctgg ccggtgacga cgtgatcaag ggcctcacct
     6181 tcttgcggtc cctcggctat gaagtgaaaa tcgggaaatc cgtatctgcc tttgatggct
     6241 atctggccgg ccccgaccgg ctgcgggcaa gggatatcaa cgaagccttt gccgacgacg
     6301 ccgtcaaggc catcgtctgc ctgcgtggcg gctatggggc gacgcgcatc ctgccgctct
     6361 tggactatga gctcattgcg gcccatccca aattgttcgt cggcttcagc gatattacgg
     6421 ccctgcatac ggccttcctc cagcgctgcg gcttctgtcc catccacggg actatggtca
     6481 tgtccctggg ccgcaatgcc tcggaatata cgaaagagca gttcgcctac ggcctgcagc
     6541 atccctatga agcgcgggaa ctgccgctgc cgccgggctg ccagccggaa accctcgtcc
     6601 ccggtgccgt cacgggcccg ctggccgggg gcaatatgat gctcctgtcg gtcctcatgg
     6661 gcacgcctta tgccatcgat ccgccagacg gcgccatcct ctgcctggaa gaagtcgggg
     6721 aagaggccta tgccctggac cgcatgctct gccagttcga ggaatcgggc ctcatcgacc
     6781 gcgtccgggc catcgccttc ggcgaattcc accactgcgg ccctgacgaa ggcgcccgtt
     6841 atgaatggac ggtgaaagaa gtcctccagg cttatgcccg caagtggggc aaaccggccg
     6901 tcatgggcct gcccttcggc cacggtgccg acaacgcctg gctgcccctg ggccagatgg
     6961 ccggtctcga agcccgcctg gacggtgtac gctttacgct gctgtaattt ggataggaga
     7021 gattctcatg gaatataaag atttgaaaga ccaggcctgc cgctatgtag atgaactgaa
     7081 accggtcatc tacggcattg ccgaaagcct tcacgaccac ccggaaacgg gcatgaacga
     7141 agtcttcgga tcggcgtcgc tgaaataatt gctgcctgtc cgcggctttg cgatcgacaa
     7201 tgccgtcgcg ggcgctttcc agacggcctt tcacgctgtc tgcggccacg gtccttttca
     7261 gatgggcttt ttggccgaat acgatgccct gccggaaatc ggccacggct gcggccataa
     7321 cctcatcgcc gccatgagcg tcggcgcggc cctggccttt gccaaagcgg ccggcgacaa
     7381 ggcgacggtc cacgtctacg gctgtccggc cgaagaaacg gtgggcagca aagtctatat
     7441 gagcgaacac ggcgtcttcg acggcctgga cgcggtggtc atcgtccatc ccggtacgga
     7501 taagacctat atcggcggca cgtcctatgc gacgcacccc atgcagttcg ccttcctcgg
     7561 caaggcggcc cacgtcgccg atgcgaccta ccacggcgtc aatgccctcg atgccctcgt
     7621 cgatttctat ggctgcctga aggcctatga aaagaccttg accgaacgcc atatcatcgg
     7681 cgccatcatc accgaaggcg gtacggcgcc gaacatcgtg cctgaccggg ctgtcctcaa
     7741 ggcgactatc cgggccctga aagtggaata cctggaagac aagatgctgc cggacataaa
     7801 aaaaatcgcc cgtgacgtcg ccgacgcaca cggagcgaaa gtggaaatgg tccattatga
     7861 accgctctat aagaatatga tcaatgaccc caggatggac gtttactttg ccgacgcctt
     7921 caaccagctc tacgaagaat tcggcatccg cgacgacgac tatgccgaag gctcgacgga
     7981 cgtaggcaat gtcagccaaa ttacgcgggt cagccagccg gaaatctgca tcggctacga
     8041 catcaatgcc catacgaaag aatttgccca agccgctggc agcgacctgg gcaagatgca
     8101 ggcattgacg ggcgccaagg ccatggccat ggtcgccctc gatgtcatgg gagagaaata
     8161 ctgattattt cgtgcaggta atgatggcct gttccaggat gtccaggccg gcatcgagct
     8221 gggcgtcggt gacgacgagc gggcagagga agcggatgac gttgtcgtaa atgccgcagt
     8281 tttccatgat caggccgtgt tgggccgctt ctttgatgac ggctgtgacc agttccggat
     8341 agggcttctt gctggctttg tcgtggacga attccaggcc gagcatagcg cctaagccgc
     8401 ggtagtcgcc gatgacgtcg tatttctgtt tccactgttc atagcgggcg cagactttct
     8461 ggccgatttc cagggcccgg tcggccaaat ggtcccgttc catgatttcg atgactttca
     8521 gggccgaagc gcaggccagg gcgttgccgc caaaggtacc gccgatgacg ccggccggga
     8581 cggattccat gatttcttcg cgggctacga cggcaccgag gggcacgccg ccgccgatgg
     8641 atttagccgt ggcgatgata tccggcgggc agccggcttc tttccagtat tccgtcgcca
     8701 gcatgcggcc tgtgcggcac cagccggtct ggacttcgtc ggcgatgagc aggatgccgt
     8761 tgtcgtcgca gactttgcgc agggccttga tccaggggat aggagccggg atgaagccgc
     8821 cttcgccctg gagcggttcg acgacgatgg cggcgacgta ttccgccggc gaagcgtaca
     8881 tgaagacttc tttcagggaa ttcaggtaga attccagggc ttcgtcatcg ctcatgccag
     8941 ccggtttgcg gtagagataa gggaattcgg cccgatagac gccgtccggg aaggggccca
     9001 tgccgcgggc gtaggctttc ttggccgtca tggccatggt catgagcgtg cggccgtgga
     9061 aggcgccgga gaagacgatg atgttcggcc gtttcgtata ggccttggcg actttgacgg
     9121 cattttcatc ggcttcagcg ccgctgttga tgaagaagga gcgcttcttg tcgcccttga
     9181 ccggcgcgat ggcgttgagt ttttccgcca gggcgacgta gccttcatgg gtgacgatgt
     9241 tggccatggc gtggaaatac ttcccggcct ggtcctggac ggctttgacg acttccggct
     9301 gggaatagcc gatgttcagg acgccgacgc cgccgaccca gtcgaggaaa tagttgccgt
     9361 ctatgtcttc aaacatggcg ccttcgccgc gctggatgac tgtcggatag atggtgccga
     9421 tggcatccgg gatggcctga cggcggcggg ccaggacggc agcggctttc ggaccgggaa
     9481 cggtctgggt aacaattttc ggtaattcat cttttaacat aatgtctccc ccttatcaat
     9541 gcaagataca aagccttggt gagtgctcta tgaaagactt tgcatggata atcttaaatg
     9601 atacgcttat tatacgtttt actattaaaa acaacaagaa gctgggaaac caaagttaaa
     9661 aatatgatgt tgtgctgacg acacaaagga gggagtccca tgtctctatc catcgctcaa
     9721 ctttacaggc aggatgaaaa caaatataaa ctggacctcc tggccggacg gcagggactg
     9781 agccacgacg tggcctgggt gcaggtcatg gaagatgcag actatggcac ttttttgcgg
     9841 cctcatgcct tcatcttcac gacgggcctg gccagccccg gcgacccgca ttggctggaa
     9901 aacttcgtcg atgccctgct ggcagccgat gcggccggcc tggtcgtcaa tacgggaaag
     9961 tacctcttgc cgtcggatat tacggaagcc ttgaaagaac gctgtgagga agcggccttc
    10021 ccgatattca ccatgccttg ggaagtgcgc ctggccgaca ttacccagtc ctttttgtcg
    10081 gccctctttt tgaccaaccg tgaagaatac cgggccatta cggcgtggaa ggaattcctc
    10141 ttcggcgtcc aggggtcgtc ggtcctgact gaactggctc tgacgggctg gcaggaagag
    10201 gggccgtata cggcactggt cctggccggc gccgatggcg atgccgattt tttggctgac
    10261 agcaaatcct tcctcaacgg gctggaccag ccatacttta tatttcctta taaggacacg
    10321 atcgtcctgc tccagcaggg aacgctgccg gcagcactcg tcgcctggct caaaggccat
    10381 gaccgcctgg tgacgggcca gggcctgacg gcgcctgact tgaaaaccct gccagacagc
    10441 tgccgccagg ggcagcaggc tttggtatgg gcccgtctcc atcagcagtc ctggtgctat
    10501 ttcggagccc tcggcgccta tgccctcttc ttttctcagc ccgatgaccg ggtgctgcgc
    10561 gtcctacacg accgggccct gggagccttg ctggcttatg atgaaagcca ccggtccagt
    10621 ttgtacgaaa cgctggaagc ctatctctgc cacgatggca gcctgaaggg aacagcggcc
    10681 cagctctaca cccatcgcaa taccgtagcc tatcgcatcc acaaggcgga gaaactcatt
    10741 ccctatgatc tggctgactg ggaacagcgg ttcacggtct tcctggcctg ccatatccac
    10801 cgttattttt ccctgttaga aaaatagtgt tgacattttg caagctaaat gctattatat
    10861 aggaaccagc tgtactgaga cggacgaaag aaacgatagg tttgttttgt tgtgtctgta
    10921 tgagttcgct tagcggaccg gcactgattg agttgaaaaa agttcaaatc agggcttgac
    10981 agacttctcg gagtttggta agatatacaa gtcgtcgcga tgaagcgatg gcaacgatga
    11041 cctttgaaaa ctgaacaatg aactgaatga acgccaacgt gcgag
//
LOCUS       DAWCYS010000031        11044 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_388_length_11044_cov_7.587679, whole genome shotgun sequence.
ACCESSION   DAWCYS010000031 DAWCYS010000000
VERSION     DAWCYS010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_11044_cov_7.587679"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2659 a         3289 c         2894 g         2202 t
ORIGIN      
        1 cgacgtatgg tgaaaacatg ggcgtcatgg ccattaccgg cgtatacagt acgtgggtca
       61 tcggcggcgc tgctgtcttt gccatcctct tttccttcgt cggcaagatc gcagccctca
      121 tccacgccat cccgacgccg gtcatgggcg gtgtctgcat tctcctgttc ggcttcatcg
      181 ccgcttcggg cctgcgcatg ctcgtcgaaa agaaagtcga ttatacgcgc tcgaagaacc
      241 tcatcctcac ggctgtcacc atgatttccg gcctcagcgg ggcgactatc gtcctcggtc
      301 cggtccagct caagggcatg ggcctggcta cggtcgtcgc catggtcatg agcctcatct
      361 tccttttctt tgaaaagatt cattgggcca acgaatagca ggccatctga aaggagttcc
      421 gtctatgact attccacaac cgaaaacccg cgacgatttg atcgcgctgc tctcgattac
      481 cgatcctgat gaactccagg cgctttatga tgcggcttat gccgtcaagg ccgccggtgt
      541 gggacgcgtc gtctattacc ggggcctcgt cgaattttcc aattactgta tcaaagactg
      601 caaatactgc ggcatccgca agagcaatga cgaagtagaa cgcttcgata cgccgcgcga
      661 agacatcctg gccatggccc gctgggcctg ggagaaccgc tacggctccc tgaccctcca
      721 gtccggcgaa cggcaggatg aagagttcat cgactacgtc gaaggcctag tccgcgacat
      781 caaggccttg agccacggcg aactgggcct gaccctctgc gtcggtgaac agacggaaga
      841 aacgtaccgc cgctggttcg acgccggcgc ccaccgctat ctcctgcgca tcgaaacgag
      901 caaccccgac ctctacgcga cgctccatcc ccaggacggc catcaccgct ggcaggtccg
      961 caaggactgc ctggacagcc tgcgccgcat cggctaccag gtcgggacag gggacatgat
     1021 cggcctgccc ggccagaccc tgggcgattt ggccgatgac atcctctttt accgggacat
     1081 ggacatcgac atgatcggca tgggtcccta cgtcgtccat cacaatacac cggtcggcaa
     1141 agacgtcatg gctgccggta tggacagtgc cgaagaaaag aaacggcgcc tggaactggg
     1201 cctcaagatg attgccgtca cgcgcctctg cctgcgcgcc gtccccatcg ccgctacgac
     1261 ggccctccag gccctccatc ccctgggacg ggaactgggc ctcaaagccg gggccaacgt
     1321 cctcatgccc atcgtcacgg tccccaaata tcgcccacag tacctcttat acgacaacaa
     1381 accctgtgtc gacgaagtac cggaccagtg caagaactgc ctcagcgccc gcgtagcctc
     1441 cgtaggcgac accatcggcc tgggacaatg gggcgattcg ccacactttt tccataagaa
     1501 atagtagagg gcgcccagag tgcgccctgc cggacatttg aacggttctc taaaaaacta
     1561 attggataat cgcacatatc ctgaatcggg cttgccacgt gcaagcccct acagattgta
     1621 aatcataaaa aggccccgat tgggggcctt tgctaattcc tagccactaa cctgctaacc
     1681 actcaaaaag ccctgtcacc tacgcgacag ggctttttgg aaattggcgg aaagggtggg
     1741 attcgaaccc acggtgcgtt gccgcatcac tagttttcaa gactagctcc ttaaaccact
     1801 cggacacctt tccataacga aactattata gcagagtttt cacaactccg tcaaggacgg
     1861 cacagggact tgctaattga cttttatatg gtatataatt aagtgtatct ttatgtctaa
     1921 atcttttgtt tatagaaaag gggcagtgat aatgtatcac ataagtgttt tgacaggacg
     1981 tcaggatgaa gatgattttg gagagagact gcgcgatgta gcgcgcgttg aatattgtcc
     2041 tcccggagat gaagaagaat tggccgacag gctcgaagat gcagatgtca tcatctgtaa
     2101 gtccgaaccg attacggcat cccttctgga agaattggac gatctgaaat tgatcgtcgt
     2161 cttatcgacg cggtcggata atgtcgactt ggcggctgcc cgggaaaagg gcgtcatggt
     2221 catcaacaat acgtcgtact gtgtcgacga tattgccgac catacgtgtg ccatgatcct
     2281 ggccctcatc cgccagctgc cggagtacca gagcgatatc cgcaccaaca gccgttggga
     2341 atacgggtcc gttccctggc ctatccaccg tgtcagcagc aatctcatcg gcctcgtcgg
     2401 gtttggccat gtcggtcgtg ccgtcgccgc ccgtctccag gcttttggat gcaagatcca
     2461 ggcttatgat ccgtttgtca gcgaaaaggt catgatggaa cgcagcgtcc gtcccgtcga
     2521 cttcgacaaa cttctccaga ccagcgacgt cgtatcactt cacatgccgc tcaacgaaac
     2581 gacacgctac atattccagg acgaacagtt cgaggaaatg aaggaaggct ccatgttcgt
     2641 caactgctgc cgcggcggcc tggcggacga agcggctctt tatcatgctg tcgacgacgg
     2701 ccatatccgc agtgccgccc tcgacgtatt gtccatggaa catccgagcc cgatgctgct
     2761 caagatgatc gcccggccgg aattcctgct cacgcccagc gtctgcttcc attccgtcga
     2821 agccgacaag gccgtccgcg atgatgccga acggtacatc cgcctgttcc tggctggcca
     2881 ttacgatgag ctgcccgttg tgaccttgcg ggacaacgaa gaatcttaac gagaggggct
     2941 gtacaatttg cgtatatgga aactggcctg cctggccatc ctgtgctgct tcctgctggt
     3001 cggctgcacg ccgacgcaga gcggcgatac ggccaaggcc gacaaggcac cgcaggcgca
     3061 ggtggaaatg gccgtgccca agcagggtat cccggtcctg atgtatcaca tgataggcga
     3121 tgtccccgat aacgatgcgg tcctcttgga atcgcacttc cgggaacaga tgaaattcct
     3181 gaaggataat gacttccatc ccattaccct ggaccagctc tatgactata tggtgcataa
     3241 caagcccgta ccagtccggc cggtcgtcct cacttttgac gacggctatc ccgatacgta
     3301 cagcatcgtc atgcctgttt tgaaggaata cggctttgcc gggacggtct tcgtgccgac
     3361 gtatgacacg gaccagggaa cgcgcctgaa ctggcagcag gtcaaggaaa tgcaggctgc
     3421 cggcctgacc attgcctccc acagttatca ccatgaacgg gcgacggaaa tgacagggcg
     3481 cacctttgcg gatgaaatca agaagagcca ggctgaactc aaggaacagc tggacattac
     3541 caacgaatat ttctgctatc cctatggcgg ctatacgaaa agcgccggtg acgcgttgaa
     3601 ggcagccggt atcaaactgg cctttaccat ggatccgggc tgggccaaat acggggacaa
     3661 cccctatgcg gtcaagcgca tctggatcgg caatgccgtc gatatagaaa acttcaagca
     3721 gcgcgtcacg acaccgcagt atgaagaacg ctaactttat tattttcgag gttacttatg
     3781 aggaaacacg taaaagaaat cggtaagttt tttgtctttg tcgccatttt ctttgccttg
     3841 acatcgccgt ttgtcgtcct cttcggcccc ttcgacaacc tgaagcgcac cgtcatcggg
     3901 gctatcctga agagccgtca tccccagtac atcacctggc ttttttcgca ggaagaaatc
     3961 gacgaaatcc tgggcggcat cagctcgacg ccgaagcagg aactgttcaa gttcaaagcc
     4021 cggacggact cgacacttac cctgaagaat attgattcca gccgtttcaa gggattcctc
     4081 ctggaaatcc cggacccgca ccgcgtacag gtcgcgacgg ctgaaaatat ggatgaaaaa
     4141 ggcgacacga ccagcggtat tgccgaacgc gtcggcgccg tagcggccat caatgccggc
     4201 ggcttctatg atgccaacgg gaccggtacg ggccgccagc cttacggttt catcctccac
     4261 gatggcaaat atctcctcgg ccaggatgcg tccgatgagg aagaaaatga attcgtcggc
     4321 ttcaccaagg acggcaacct catcgtcggc aactactcca aggccgaact gaaagaaaag
     4381 gacgtcgtcg aaggcatttc cttcgggccg gccctcatcg tcaacggtga aaagatgatc
     4441 acccacggcg acggcggctg gggcgtcggt ccccgcacgg ccatcggcca gcgcaaagac
     4501 ggcacggtcc tcttcctggt catcgacggc cgccagcctg gctattccgt cggggcgaca
     4561 ctgcgcgaca tccagaacat catgtatgaa gaaggcgccg tcattgcggc caacctggat
     4621 ggcgggtcta gctcgacgct gtattataat ggtaaagtca tcaacaaacc ggcggacctc
     4681 ttaggtgaac gcatgattcc gacggcgttc gtcgtcaagt aggaggcagg ctatgaaatt
     4741 acgaaagttc attgcagtct ttctggccgg catcgccgtc caggctgcta tttatctcta
     4801 cctggaccag gtcttgttcg cgccgacgtc ggatttccag atcggcggca cgaaccagca
     4861 ggaagccatg ggcgaaggga cagaaccgaa cggccgcagc ttctattcct atgacaagcg
     4921 cttcatggcg accatcgtcg acgacagggt caccatttac gaagccggca agaagaacga
     4981 tccccaacgc atcaatctcc acggccgcaa ggtctccttc tttgaatggc tgcccgaccg
     5041 caacctggct atcttcgcca cctatggccg cgacaggaag accggcaagt acggcgtcta
     5101 catcgcccag tacaacccga tttatccgga ccgcgaactc gatacgccta tcgaagatgc
     5161 cccggtcgac agtaagatca ccgatgtcgc ctattcgacg gcgaccaatg tcgtctacat
     5221 gaagctccag gtcggcgaca ataaataccg catctaccgg accgacgcca actacgacac
     5281 gcgccgcatc cacgtccagg cagaaaacat cggccgcatc gccgtattct acgattcgga
     5341 tacgttcttc tacgacaacc tgcggaccgg cgtcgtctac atgttcgacg gggcgaccag
     5401 cagctggcgt gaaatctcgc cgagcggcaa tttccgtctc atcggtatcg atggacagga
     5461 aatctacatc gccgaaatga accacgacga caaggtcgta gccgcctacc gcggccgcct
     5521 gcgggtcggc ttcaagaaga tagcgacgta caagacgccg gaagacttca ataagattac
     5581 ccttaacagt atgcacgaag catcggaaaa atccgataaa atggaaaaga agtaggtgat
     5641 tgtcatggca aaacataaga ttcccaagaa tcgctctatc gaagacatgt tccagggcgt
     5701 cgaagaagaa tcgacctttg aaacgacggg ctgggaagac gacgaagtga gcgtccgccg
     5761 gccgcaggcc aaaaagacgg ctaagaagca gaccgcctcg gacttccgcc gccagttctt
     5821 cacggacgac ctccaggaaa aagtcggctc tatcctcctc gatatcaaga tggcttactt
     5881 caaggacggc gtcggcgaca tctccctgga agtcgtcaag gacggccgca acgtcgtcat
     5941 caagacggca ccgaagacga gcaaaaaact gagaaaatga aacaggcaga cctggaaaag
     6001 catttccgct gtggtcccgt cgaggcggcc tatgagaact cgaaatggtt ttccggcttc
     6061 atgaaggtcc tcttcctcat ggtggccatc tacttcttct ggctcatgac gggggaagcg
     6121 cacctggttg aacacctcga caactgggcc gtcctggcct tttgcctgtg gcgcatccat
     6181 cgccaggacg acgtcatgtg ctacgtcacc cggaagggcc tcatcgtccg ccgccagttc
     6241 cggtcgctcc atgaattcta cgacgaccag ttccatgagg agcggagcct catcttcctc
     6301 ccgtataagg acatcttcgt catcagcgac aactggcagg aaatccagct gggcgaagcc
     6361 gaagaaggcg gcctggccgt cctgcccgtg cacctgcagt tcctgtccaa gcgcaacaaa
     6421 cagcgcatca tcgaccgcat caagcaggcc catgaaacgg acgatgacga aagcgcccat
     6481 tgacaggacc tgtcctatgt gcgatatgca tgaatcggca tggagacatg ctgatttttt
     6541 atacccaaaa gcctttatct ttttaacgaa ataaagtaaa atagggcatg tacacaacgg
     6601 tacgcctaga tgatctcaag gggagagcga taatcatgga atggaaaaaa tacgcagctg
     6661 ccggtatggc ggcaattctg acagcaactt tcgtcgccgg ctgcggcgga gacaaacagg
     6721 ccgacaaggg gacggctgcc aagagggata agattgtcat cggcctcgat gacaacttcc
     6781 cgccttttgg gttccatgat gaaagcggcg agctcgtcgg ctttgacatc gacatggcca
     6841 aggaagcctc caagcgcctg ggcatggaag tcgaattcaa gcccatcgat tgggacagca
     6901 aggaaacgga actgaagagc aaaaaaatcg atgccatctg gaacggcctg tccatcacgc
     6961 cggaacggga acagaacatc ctcttctccc ggccctatga aaacggcccg cagatcctgt
     7021 tggtcaagaa cgactcgccc atccagggca aggccgacct ggccggcaaa atcgtcggca
     7081 cccagcaggg cagcaccggc ctggaagcca tcgaagccga accggaactg cgcgattcct
     7141 ttaaggaact gaaacagtat tcggacaacg tcacgtcctt catggacctg gaagtcggcc
     7201 gcatcgatgc cgtcgtcgtc gcccagacga cagcggcgta cttcatcaag aagaacaacg
     7261 cgccgttccg catcatcgac gtcggctatc ccgacatccc cagcggtgtc ggcatgcgca
     7321 aggacgatac ggcactgaag gcccagctcg acaaggtcct ggaagacatg cacaacgacg
     7381 gcacgtcgaa gaagatttct gaaaaatggt ttggcatcga cgtcacgatg taaggaggaa
     7441 ttcacatgaa ttggaaaaaa gcactggctg cgggtacact ggccgtagca gccgttacca
     7501 tggtcgccgg ctgcggcggc aatgcaaata aacaggcaga acagaaactg cccgataaga
     7561 tcgtcatcgg cctggatgac aacttcccgc ccatgggctt ccgcgatgac agcggtcagc
     7621 tcgtcggctt cgacatcgac ctggcacagg aagcgtccaa gcgcctgggc atccccgtcg
     7681 aattcaagcc catcgactgg gacagcaagg aagcagccct caagagcaaa caggtcgaca
     7741 tgctctggaa cggcctgacc atcaccgatg aacggtccca gcagatcgcc ttctccaagc
     7801 cctatatgaa caacgcccag ctccttgtcg tccgcgccga ttcgcccatc aatgaccggg
     7861 ccggcctggc agggaaggtc atcggcaccc aggaagggag cagctccatc gacgccctgg
     7921 aaaagaacgc cgaattcaag gattccctgg cagaagtcaa gaaatacggc gacttcgtca
     7981 acgccttcat ggacctggaa atcggccgta ctgacggcat cctcgtcgac agcgtcgtcg
     8041 gccgttacta catggccaag aaaccgggca agttcaaagt catcgacgac aagatgggcg
     8101 acgaaaaatt cggcgtcggc atgcgcaagg aagatacgct cttgcaggat aaactcaacg
     8161 acgtcttgaa acaaatgagt gaagatggta caatgactaa gttgtcccag aagtggttta
     8221 acgaagatat taccattaaa gtacagtaag cgaagcggga tgtaacgacg attcgtaggg
     8281 gccgcccaaa agggcggccc gccttgcgta tatacgtatc ctatgctgta tatgtgtcaa
     8341 ttccaaaaaa aggagccaac atggattatt tatttcacat cgtgcctgtc gttgccgtag
     8401 gcctgcagat taccctgaaa attttcatca tcaccatcgt cctgagcctg cccttgggca
     8461 tcctcatggc tatcggccgt atttccaaga tcgccatcct gcgtaaggcc atgtacgcct
     8521 atatctacgt catgcgcggc acgccgctca tgctgcagat cctcttcatc tactacggcc
     8581 tgcccttcat catcgacggc ctgaaactgc cggccttccc ggcggccatc atcgccttcg
     8641 tcctcaacta tgccgcctac tttgccgaaa tcttccgcgg cggcatccag tccatcgacc
     8701 gcggccagta tgaaggggcg aaagtcctgg gcatgaccta cgtccagacc atgcgccgca
     8761 tcatcctgcc ccaggtcatc aagcgcgtcc tgccgccggt ctcgaacgaa acgatcaacc
     8821 tcctcaagga tacgtccctg gtctacatcc tggccatgaa cgacatcctg cgcattacgc
     8881 gctccatcgt acagcgcgac ttcgacacga cggccttcct ggtcgctgcc gtcttctacc
     8941 tgttctttac ctttatcctg accaaggtct ttggttatct ggaaaaacgc tatgccgttt
     9001 acgatgaata aggaggatta tcatgagttt tattgaaatg gaccatatcc gcaagagctt
     9061 cggcagtctc gaagtcttga aagacgtcac cctccacgtt gaaacggggg aagtcatttc
     9121 cattatcggc ccgtctggca gcggcaagag taccttcctg cgctgtctct gtcagctgga
     9181 aacgatcaac agcggctcca tcgtcgtcga cggccagacc atggtcagca cgcccgaagg
     9241 gagcagccgt gccgtctacg ccccggcagc cgacgtccgg accatctgcc gccgcatggg
     9301 catgtgcttc cagcacttca acctcttccc gcacatgacc gtcctggaca acatcctgga
     9361 agcgccgcgc atcgtcaaga agatgaagac cgaagaaatc atgcccaaag ccgaagaact
     9421 cctgaaaaaa gtcggcctct gggataaacg cgatgcctat ccgtcgcgcc tctccggcgg
     9481 ccagcagcag cgtgtggcca tcgcccgcgc cctggccatg gacccggaca tcatgctctt
     9541 cgacgaaccg acgagcgccc tggacccgga actgaccggc gaagtcctgc ggaccatcaa
     9601 acagctcgcc gacgaacaca tgaccatgat catcgtcacc cacgaaatgg ccttcgccaa
     9661 agaagtcgcc gaccgggtca tcttcatggc cgacggcatc atccaggaac aaggcaaacc
     9721 ggaagacgtc ttcgaacacc cgcagaacga acggacccag aacttcctca aatccatgct
     9781 ccatttttga tggtaaaatc ataattcgca gttcgcaatt cgcaggccgc aggccgcaag
     9841 gctcctctaa taggggagct ggccgccaaa ggcggtctga gaggttgccc atggaccgct
     9901 tgtgggaatc ccgtggatcg tattgtaggg gcgcctacgt ggggcgcccg ccggtatata
     9961 aaggaatccc gttatcaacc atttacgtga gcgggctcgc cacgtgcgag cccctacagc
    10021 aaaaggactt gtcgtcatgc gacaagtcct ttttccacat cggaccgttc gccgtccagg
    10081 aggtagcccg tgcgccccgt tttgtgctat aatgatttca tacgcgaata tgacattatt
    10141 ctatcgaaag gtagggaact tacatgactc aattaaaatt acgttctggt tttacttttg
    10201 acgtaaccaa cctttatggt cccaaggccg tcactgaaga agaagtcaag gcttttgaac
    10261 ccgtttacac caaggctcac gaagccgtta tgaatatgcg caagaccggc atcatgccgg
    10321 gccatctctc gaaagacgga gaaccggaag aagtcctctt cccgcagctg ccgtacatcg
    10381 aagaaggcaa catcaacagt ccggaacgca tctttgccct ggaagaactg gcacggacga
    10441 cgcgcaaccg cgtagacacc gtcgtattcc tcggcatcgg cggttcctat ctcggtggca
    10501 aggtcctctt cgacgtccag tgcggcgaat tctggaacct caagagcacg gaagaacgca
    10561 agggcttccc gaaagccttc tttgccggca acaacgtcga ctcccataag atgacgggcc
    10621 tcatcaattt cctcaaggcc gacagccaca ccaaaccgaa ctacacggtc atggccgtcg
    10681 tcatttccaa atcgggctcg accattgaac ccatgtccaa ctacatggtc ctcaaacagg
    10741 ccatgaacga tgccggcatc gccctggaaa cggtagccgt tacggacccc cacgacggcg
    10801 gcgaaaatga cacattgctc cacggcgtgg ccaagaaatc gggctggccc atcttctacg
    10861 ttcccgatgg cgtcggcggc cgtttcagcg tcttctccga agtcggcctc atcgtcggtg
    10921 ccctcacggg ctttgacatc cgtcagttcc tcgacggcgc ccgcgacatg gaccacgcct
    10981 gccagaaccc ggatatatgg cagaacccgg ccctgctcaa cagtgtcctc aaattcctgg
    11041 ccgg
//
LOCUS       DAWCYS010000032        10901 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_393_length_10901_cov_8.093398, whole genome shotgun sequence.
ACCESSION   DAWCYS010000032 DAWCYS010000000
VERSION     DAWCYS010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_10901_cov_8.093398"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2628 a         2575 c         3052 g         2646 t
ORIGIN      
        1 agtgggtaat tgaagcgccc ttccttatcc cggctggaca ggccttgggg attcctgatg
       61 cgcagactgt cattgcctat atgtttggcg gggacttgac ggcattagta caaccgttct
      121 ttgccgttcc gtttatggct gtttgcggat tagagttcaa agattttgta ggatatagtt
      181 tcgttgcctt tattgtatta ggcatcatca tggttgtcgg tttaacggtg attccttttg
      241 ctatttaaaa ttatacgtga tagaaagaag gtagaagtat gaagcagttg aaggacatta
      301 aacggatttg taacttaggt tgtggcacga tggggtttgg cacagcgatt gcttttgctc
      361 agaatggcta tgaagtaaat atgtttgggc gtaaagatgc cagtattgct cgggctatgc
      421 gaaatataca tgctacatta gatgtcatga aagccaatga tttgctgacc gaagcagaag
      481 aaaaagaatt gctcagtcat attcatggtg ttacatctat tgaagatgct gcaaaggatg
      541 cagattttgt ccttgaatca gtagcagaag acatggatgt taagcaatat gtttatggag
      601 aattggaaaa atatcttggt ccagatgtta tttttgctac ggactcttct ggccttttgc
      661 cgacagaagt cgcctctgtt ctcaaacatc cagaacgttt cgtcgttgct catttttgga
      721 acccgcctca ccttattcct ttggttgaag tcgttccggg tgaaaagaca agccaagaaa
      781 cggtagatat tacttggcag cttatggaaa aaatcgggaa aaaaccggta gctcttttga
      841 aagaagctcc aggattcgta ggaaaccggt tacagtttgc cctgcttcgt gaagcactgt
      901 attgtgtaca agaggggatc gcgaccgccg aagcggttga tctcatttgt aaatacagca
      961 ttggccgccg gttaggcgtg acaggaccgc ttgaaactgc tgatttagga ggccttgata
     1021 ttttctacaa catttcggcc tatttgaatg ctgatttggc tgatgacaaa gaaggctctg
     1081 ccgtcatgaa aaaatgcgtt gatgaaggaa acttgggtgc aaaaacaggg actggtctat
     1141 accagtggaa accggaagaa ttggatcata tcaagaagac ccgagaagaa gttttgattg
     1201 aatggttgaa aaaagataag gccggtcaga aattctgacc gttacttata tacatttgtc
     1261 ctccttcgag aacagggact actgcaagtg cggtagtccc tgttctttat ttgctatttg
     1321 cgtttattta gctcgctatt ttttttatgg acgatatatt atagttttgt tatacttttt
     1381 ataggtgcta ccaaaacggt aggcggttat ccctcttcgg agggcgctat accctccacc
     1441 cagattagaa atccgtattc acataaagaa aaataaccgc ctcgctccca acgcaacggt
     1501 tattttttct ttaaataatc tttgagggct aaccgtctat cggtagcgcc tttttctata
     1561 ttcagtttag catttatagg agaagccgtc aatagcccct tgagtgaagg tcttgtaata
     1621 ttagggacat agaaaaggag ctaccctgat gaggtagctc cttttttgct atgcggttat
     1681 tttgccagcg tatgggcggc tttgatgcgg tgcgtgatat aggtgctcag cagggcgccg
     1741 aggatggcaa agacggtcat gacggtgaag acgatttggt agccgtcgat gccctgatgg
     1801 ctatcgagga tggagccgta gacggcgtac atgaaggcgc cgggcagata gccgatgaag
     1861 gaggccatgg acatggcaga gcctgtgatt tcgcggggga cgtcgacttc atccatgggg
     1921 gcgaagaaga cggcgcgcat gctgtagacg caggcactga tggccaggga gatgatgatg
     1981 ccggcccaga tgccgaaggg tctggtaggg catccaggtg aaggcgatga ggacgacggc
     2041 tgtgatcaga taaccgatgc gcaggtattt ggtcggcgaa tggaagagtt tgtccgataa
     2101 caggccgccg accattttca ggccgtactg gttgatgatg ccgtagacgc cgatgagcct
     2161 gaaatacatg aaatgccatg gcagccactg aatgcggatt cctatttgac agattgaagt
     2221 ataagaaaat ttaggaagtc cgcatctgtc ccaggtatgg tggacggtat tccaagattc
     2281 agtattgcta ctctctcaat cccctcttgt cccagctcta tctgggacgg gccgaagtct
     2341 atcattctga gccgttgtgc ctggaagtga cgccgtgccg cgtcgataaa tcctttggct
     2401 tgaaaaaact cttctcctcc cgcgttcatt gacatcctct gcggataagg gtatcatgaa
     2461 ggcatgtgaa gaagtaatgg cattatagga ggaaggacat gtttggctta ggcgcaccgg
     2521 aattactgct cattttagtc atcgccctgg tcgtattcgg accggggaaa ctgccgacca
     2581 tcggccgggc tttggggaag agcctccgtg aattcaagga agccgtcaat acggatgaaa
     2641 caaagcagat agaggaaaag acggaaaaaa cagatgagaa atcgatgaac cactaattgg
     2701 cttataacgc aaagtttgta gtatggagca gtttgtagaa gctggctcaa attttttacc
     2761 atttgctgca aactaaaaac tacaaactta aaactcaaaa aagggctgtc gcacgtgcga
     2821 cagccctttt tcgttttctg gcgggaaagt gtgggaatcg aacccacctg agaagctgtt
     2881 aactccccat gctggttttg aagaccagag ggaacaccag aacccatcct cccccaggac
     2941 aggtttatta tatcacgggc atcctgtatc ttgcaaaact gtttccatct ttgttacact
     3001 aaaggcggtg agtgcattac ccattagaaa ggagttaatg aaatgattga attacagttg
     3061 aaaccgacga tttactctta cgatacgtgc cgggaatttg tggaagacct gcaggtcgga
     3121 gccgatgacc tgattgttac caatcagtat attttcgacc cgaatttcaa ggatttaggc
     3181 cttacgtgcc acgtcttgtt ccaggaaaag tatggtatgg gtgaaccgtc tgatgaaatg
     3241 gcagaagcca tttataaaga tatgcccaag gatgtgaagc gcatcatcgg tatcggcggc
     3301 ggcacggtca tggacttgtg caagttgttc atcttgaagc aggtcagccc ggtcctcgat
     3361 ttgttcgatg gcaagattcc tgtggaaaag gaaaaggaac tcatcctcat cccgacgacg
     3421 tgcgggacgg gcagtgaagt caccaatgta tcggtcatgg ccctggttag ccgcaacacg
     3481 aagaagggca ttgccgacaa tgcgctctat gccgataaag ccgtcctcat ttcgcagctc
     3541 ttgaagaccc tgcctttcaa ggtctttgcg accagttcca tcgatgccct cgtccacgct
     3601 gtcgaatcgg ccttgtctcc ggattccagc gccagcttcc gcgttttcag ttataacgcc
     3661 attgaactca tcctcaaggg ctatttgcag atccgcgacc atggtccgga agcgcgcatc
     3721 ccgcttatga aggctttcct cctggccagc aactgggccg gcatcgcttt cagtgtccct
     3781 ggctgcgccg ctgtccatgc catgagctat cctctggggg caaagtatca tgtacctcat
     3841 ggtgaatcga actacgccat gttcacaggc gtcatgaact gctacatgtc catcaaatcg
     3901 gacggtgaaa tcgcgaaatt gaacggcttc atcgccgaca tcctgggctg cgaaccggaa
     3961 cacgtctatg aagaactgga aaaactgctc aacgtcatcc tgcccaagaa agcgctccat
     4021 gaatacggcg tcaaagaaga agaattgccg gaatttgcta aatcggtcat cgaaaatcag
     4081 cagcgtctca tgaagcacag ctttgtgccc ctcgattatg ataaggtgta cggcatttac
     4141 aagaaactgt attagtggct agtggttagt cgcaggggcc gcacgtgggc ggcccgtctc
     4201 gggccatgtg ggaatcccgg gagagccatt agccgctaac tgctaaaaaa agcgctgtcg
     4261 catcgcgaca gcgccttttt tgtaagggct cgtggctcac gcccgttggc tctcttatga
     4321 ggagagcagg cagcgccgct gactgaaggg ttcaacctct ccggccctgc gggccacctc
     4381 tcctatcagg agaggcttgc ctgcggcctg cggttttaca cattagaacc agacggcctg
     4441 gtcgccggct tttacgttgc cgtcggctgc caggtggact tcctgaccgt cttcgaggtc
     4501 ggtgaagatt cacaggcagg cgtcagatgg ggcgttggcc ttgatgaatt cgtcgtcgaa
     4561 ttccatcagt ttatcgcctt tcttgacttc ctggccgttt tcgacgaaga cattgaagcc
     4621 tttgccttcg agtttgaccg tatcgatgcc gatgtgcagg aggtattcta cgccgtctgc
     4681 cgtggtcata ccgatggcgt gtttcgtatc gaagacgaag gtgactgttc cgtcttccgg
     4741 cgcatagacc gtgccgtctg tcggatagac gaagaagacg tcgcctgtca ttttgccggc
     4801 aaaggcttca tccggggctt ccgtaatggg atggaggctg ccggtgaagg gcgaattgaa
     4861 gtaatgcttt tcagcatctg tttccggttc agtctgtgtt tcctgggctt tcggcgcttc
     4921 ttcagccgga accgtttccg ctgccggtac ggcttcgata gggccgtctg ccgggttggt
     4981 tgccagatag ctttccaggt tggccttgat gacggcgact tgcgggccgt agacgacctg
     5041 gacgccctgg cctttcttga tgacgcctac agcgccagtg gctttcagag ctgcatcgtt
     5101 gaccaaggcc gaatcggcta cggagcagcg caggcgcgta gcgcagcagt cgacggaagt
     5161 gatgttcttg gtgccgccca gggcgcgggt gatggcctgg ctgacgacgt ccgtggcagc
     5221 ctcgccgtcg ccggctttgg ctgcggcgtc acgggctttg acgtcggctt tggtgtagag
     5281 tttcgtttcc acatcgtcgt cttcacggcc cggtgtcttg aggtcgaact tcttgatgag
     5341 gaacgtgaag atgaagtagt agaggaagaa gtagatgatg ccgaccggga tgatgtacat
     5401 ccagttggtc ttggcttcac cctgcaggat accgaagatg aacaggtcca acaggccgcc
     5461 agagaaggtc aggccgacgg cgatgttcag catgtgggcg atcatgtaag cggcaccggc
     5521 caggacgacc tggacgtaga agagcatcgg tgcgacgaag aggaaggaga attcaatcgg
     5581 ttccgtgatg cccgtgaaca tggacgtcag ggctgccgat aagagcaggc cgccggcgac
     5641 tttgcgtttt tccggttttg ccgtgcggta catggccagg gcggcgccgg gcagaccgaa
     5701 gatcatgaag atgaattcgc ccgagaaata tcgggttgca tcagcactga agtgagtgac
     5761 gttaggcgaa gcgagctggg cgaagaagat gttctggccg ccctggacga ggttgccgtc
     5821 gatcatcatc gaaccgccga cgccggtctg ccagaagggc aggtagaaga cgtgatgcag
     5881 gccgaagggg atgagggccc gcttgatgat gccgaaaatc aaggtgccga tgtagcccgt
     5941 acccgtgacc aggccgccga gggcgaagat gccgctctgg acgacaggcc agacgaaata
     6001 catggcaatg ccgacgaaca tgtagacgac ggtggaaatg atagggatga agcgcgaccc
     6061 gccgaagaag gacagggcat tgggcagtac gattttatga tagcggttgt gcaggtaggc
     6121 gacgccgatg ccgacgatga taccgccgaa gacgccttcc tggagggaca tgatgccgca
     6181 gacattggca atcgtacctt ccaggacatc gggagatacg gtgttattgg ccaatacctt
     6241 gccagtcagt ttgaggacgg cgttggtcga ggcgtgcatg acgaaaaagg cgatcatcgc
     6301 cgataaggcg gcgacttcct tttcctgctt ggccatgccg atggcgacgc cgacggcgaa
     6361 gatgatgggc aggttaccga aaatgatgtt gccggcatcg ctcatgatgg tgagcagtga
     6421 atggagtatc gtgccgtttc cgaggatgct ttccaatcca taggtctgga tggtcgtagc
     6481 attcgtgaaa gaagccccga taccgaggag taagccggca atcggcagga tggcgacagg
     6541 cagcatgaaa ctgcggccga cagcctgcaa gatggaaaag acttggtctt tcataaaatt
     6601 cctcctttta ctataggaat aaataagtat gagacagtac gttcctattt taacataccc
     6661 taggcaaatg agaaggatga gagttacgtc taaccgtacc tatttcgcat atatacttga
     6721 caaaaagtaa acgcaagttc atcgtcgaaa cactagctct tttaagccgt cagcagctag
     6781 cagttagcgt taaaatttaa agccatccgc taaccactaa cggctagcca ctagctacgt
     6841 aaaagggctt gtcgttgtgc gacaagccct tttactaaga ggagaatgaa tgtgaagttg
     6901 ttttctgatt tttatgcttt ggcagcggct tcgcccaatt ctttgcattc aggagcgttg
     6961 tccggtgttt cgttgacgat ggccgtacca atgacggtgg caccggcatc ttctgtacgc
     7021 tgtttccatg cgtccatcca ttcgccggaa ccccagccgt aagaaccaaa gaggccgact
     7081 tttttgccct tcaatttcgg agccaaatcg gtgaagaacg gttcgacgac agagtcttcc
     7141 agttcttctg aacccatggc cgggcagccg agcaagatga cgtctttgct agctacatca
     7201 tcgacatttg tatcttcaaa gcggactgat tctacgtcag caccggcagc tttgacggct
     7261 gcttcgattt cgttagccat tgcttctgta ttacctgtac cactccaata aacgatttct
     7321 accatgatta aatcctcctt atgctacaat gagcgagtct aatgttgtcc caacggcaaa
     7381 gcgattcaaa tagtcttgcc ggcagcacgt atagcgatgg aaagtacggg cggcagcgtg
     7441 ggcgtcgccg tataccttcc attcgccaca aagcttggca tggtttccct tgttggcgat
     7501 gaaaccgcac acgtcctcta ggggatagcc gaggaaaatg cccgattcat gagggaaaca
     7561 gccatcttct gcaaagcgtt cggcaagacg gtccagcagg gaagagatgt cctggcagtc
     7621 ctgatagccg aattgctgca gaaatgatga aatgtcccgc tgctgtacgt aggcccagac
     7681 cctggccggt cggtatacat acagcaaagt gcgtttggaa caggcgtaca agatacggaa
     7741 atacaagcct tttgcattgt atttatgatt atacttttca attatatcat cataggtaca
     7801 gccgctgtcc aagcggggta gacagagaag ggatgcctgt ttgagacccg ctaaagtcgg
     7861 tgaacagtgg gttattaaga cgaaatccag gttcatatcc ggttgccgct cctttcgctt
     7921 tgatactgta agtataattt atttgaaagt gattgtcaat actaatatga taattcgttt
     7981 caaataagcg aaaggcttgc caagtatagt ataataaagg tagtgtgaag aatattcgga
     8041 ggggggggat tagatggaaa ctgcttatga tacgaatcat tgtttcggct gtggccgcga
     8101 taacccgatt ggcctgaagc tggacatgaa gatggaaggc gatcactgcg tggcttattt
     8161 tacgcccaag gcagagcatg aaagttatgg tgaccgcatg cacggcggcc tgacgagtac
     8221 gctgctggat gaagtcatgg gagattatgt gttccgcatt gccggtaaac cagcctatac
     8281 ggcgcggctg gaaatccgtt tccgcagtgc catacgcatc ggcgaaacgg tcaaggtcga
     8341 aggctggccg gaagtccacc gcggccgtct gttcatcatg aaaggaaaga ttacccacgc
     8401 tgatggtacg ccggctgccg aagccaaagc ggaaatgatg ctggctaaat aaaggaggag
     8461 ttactatgga tagaagcgat atgatgcacc gcatgtatga tattaaaggg cctttagtgg
     8521 ccaagaagtt tgcagcccgc ggctttgaag ccgtatactg tccgacgaag gaagaagcct
     8581 tgaagcaggc catctcgtat atcccggaaa cggatgtcgt atcctggggc ggctctgtat
     8641 ccatcaatga aatcggcctg cggccttatg tactggaaca caatccggtc atcgaccgcg
     8701 attcggccaa gacgctggaa gaacgggtcg aactgatgcg caaggccctg ctctgtgaca
     8761 ccttcctcat gggggccaac gcagccgtcc aggacggcca gctcatcaac atcgatggca
     8821 acggcaaccg cgtcgccgcc ctttgctacg ggccgaaaca ggtcatcgcc atcgtcggta
     8881 tgaacaaggt cgtcccgacg gtcgaagccg gcatcgaacg ggcgcacacc gtggcgtcgc
     8941 cgcagaatat cggccgcttc cccggtgtcg gtacgccttg tgccaagacg ggcatgtgtg
     9001 gccactgcat ccggccggac agcgtctgtg cccaagtcgt ccggacgcgt atctgcaagc
     9061 cccagggccg catcaaggtt atcctggtcg gagaaaatct gggcttttag tcctgaaaag
     9121 agaggtattt tattatgaga actggaacgg gcgtcgtcga agaagtctgc ccgaatggcc
     9181 tggcgatcat ccagaccacc cgcaataatc tatattggcc gtgcagcgac agcatgtgcg
     9241 cggacaatgt cgtcatcgat gctgtgaacc ccatcggggc caaaaaggga cagtatgtac
     9301 agttcaacat ccccgacgcc ggcatgggcg tcaagggcat catctgcttc ggcgtcccct
     9361 tgctgctcat catcgtctgt ggcgtcgcag gctacctgct gggagaatca tggcaggtca
     9421 aaccggaaat gacggccttt gccggcatga ttgcaggcgg catcatcggc tgcctggtcc
     9481 tgaaaggcta tgaaaagaaa atcaacaaca accagaccaa agccgaaatc acgggaatca
     9541 tcagttaaga ggctgcaggt tcgtaggggc tcgcacgtgg cgagcccgcc agatatatgt
     9601 aagaattccg tgaacaattg gcatgagcgg gcgccccgcg tggcgcccct acagaaaaag
     9661 gcttgtcgca cgtgcgacaa gccttttttt atacaatatc gatttggcag gctttcatgg
     9721 cctgtaatgc tctttcgtgg ttttctggtg tcgaaccggc acagcatgtg ctgtcgacga
     9781 tgatttcctt ttccgggtag aaggctttga gcagcatggc attggcgatg acgcagatgt
     9841 ccgtgcacaa gcccatcaat accagccggt cgtagtcgcg tacttcatcg accagggccg
     9901 gacagccaaa ggatggcttt tccaggatgg tggcccacat ggtgtacggc aggagctgtt
     9961 tgcagatgcg ccagccccgc gtgtctttca ggcagtgtgg aacgggaagt ttccggcctt
    10021 cctgggtttc gctgtaggcc tcgtcgtggg tgtcctgggt gaaaatcagg tccgtttcct
    10081 ggtcggcatc ctttttgctg gccacgatct tggcctcgat gcgggggacg acggcttgag
    10141 cctgagcgct tcccagggcg ccgtcgatga aatcattctg catatcgatg atgatataag
    10201 cctgggtcat tacggtcatc ccttctcttt aggcacgcaa ttcttttaaa tatcccggta
    10261 cgtccgggca ggtcatgagg cccgacatga gggcgacgcc tttgatgggg acgtcctgga
    10321 gctgagagat ggtcttcggc gtaacgccgc ctaaggcata gaccggaatc ttgacggcct
    10381 tgcagatggc ttcgaccgtg tcgacgccga taggcggatc cgacggcttg caggctgtcg
    10441 ggaagacgtg gctggccatg aggtacgtag cgccgagctg ttcggctttg acggcttctt
    10501 cgacggtgtg tacggagacg ccgagggtcg tcatgtaata gagcagggac gaatgggttt
    10561 ccaaataagg cagagagcac tggaaacggg gaatatgaag acggatagca gcctttccga
    10621 aatgctggag gatacacgtc ttctgatgga tgctgcacag gcgcagggcc cgtttcgcat
    10681 agtccgtgta ttcttcttca gaaaggtttt tttcacgaag gacgatgaaa tctacatcgg
    10741 atgcggcgat ttgctcgagc tgatcccagt agttcgtcga agccaattcg tgatttgtaa
    10801 ttgcagctac ttttaacacg atttatcacc tgaatattag agtattttat atatataact
    10861 atacatgaat ctcatactaa cgtcaacaat tataccaata g
//
LOCUS       DAWCYS010000033        10745 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_400_length_10745_cov_6.603835, whole genome shotgun sequence.
ACCESSION   DAWCYS010000033 DAWCYS010000000
VERSION     DAWCYS010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_10745_cov_6.603835"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2117 a         2989 c         3158 g         2481 t
ORIGIN      
        1 taaagactcc ttactggtta ctgctgctgg agctgtccga actgctggag ctgttggcgc
       61 tgctgtcgga gctgctggaa ccagagttct tgttcagttc tttagcgacc tggtcggtaa
      121 tatccgtacc gccatagagg acgacgctct tgtcgacgac gaggctgagg cctttggtct
      181 tggcaacatc gctgacagcc gtgtcgactt tatctttgat gcctttgaag atttcctggc
      241 gtttcgtagc cagttcgccc tgcatcttct ggaagagctg ctgtttctgc tggtcgtcca
      301 tgccggccga ctgctgttca aactgtttct gcatgtcttc gccagcctgc tggagctgtt
      361 tctgtgccgc aacggcgtcc tgattatcag gagtgatgat cttgctcgta tcgacgacgc
      421 cgatgttgct cgacggagcc gcattgacag ggttgcccgt ctgcatgata gccaggccgg
      481 cgacactgaa gacgaagata gctgcgataa cagccgaaaa aatcttgata ttgcgtttct
      541 tacctatttc agacatcata ataaaacttc tccttttgta ttacaaataa ccgataatcc
      601 ggagccaagc atcgtggtcg gacacgatat attccaaacc gacataatca tcgacgcgat
      661 agtgcaggcc gacttcggaa tcgtcatgcg tcaggtcccg gtcgtagcgc aaggtccacc
      721 gcccgcctaa gggctggcgc agccacaggt gctggctgtc gtcgaggctc tcgaactggt
      781 atcccgcctg tgttctgccg gcaaagcggt aaaagtagcc gggaatgaaa ttccagtcca
      841 gcgaagacgg catgaacttg acggcgccgt agatttcgtg atgactgccg atcttgcggc
      901 ccagatgggc catgaggacc gtatcttcgt cgctgctgtg gtcacgggcg gcgtcgatat
      961 aggctccgcc gcggatgtca taaaaagccg tcttcgacgt aaggtcgatg gtcgtatcct
     1021 caccgagcga caaggacggc gtcacgtgaa ggccgtagtt cttgacgacc cactgacgct
     1081 ggacgtcttc ctgcaaggct gcggcgatgt cccgctcatg gcgcttaacg aaggcgacag
     1141 gcaggccgtc cagtccggcg tagtcccgtt ccagatggtt gcgggcgctg aggaagatga
     1201 ccttgggcac atcatcgccc ttgatggcga cgcggacgtt gcgcacgaca ggcagcttgg
     1261 gcagcatata gaccgtgacg tctgtctcgg gcccgccgtg gatgacgatg tgcggataga
     1321 attccggcac ccggtcttcc agttcattct ccaggaccga cttgacggcg ccgttagccc
     1381 aatccagggc atcgacgggc agtcccgtga gcaggctctc agccatatcc ccggcgtgtt
     1441 ccaggtcctt atcgaccagg gcctggccca tcgccggcag agcgccgtag tcgataacaa
     1501 agtggacttt gcggatcgta tcgccccagg ggcggatgcg cacatccagg tccgtccggc
     1561 tgccgggatg gatggcaagg gattccaccg tatagcctat gaggacgcgg ttgatgatat
     1621 cgttgacggt tcgctcgtat tcttcatgac ggctgacgat ttccccgctg tcgtggttga
     1681 ggaatatgtg gttccctaca gtttggatgc tggcagcaat gcgtttctcg actaatggcg
     1741 gaatcgcctc ctctacgtag gcgacatgga ccgtcacttg gtctaccgat gctgctgtag
     1801 cactactaag actagcacag acggcaaaga cagtcaataa ataagtgcgg atcttcatca
     1861 tccacgacct ttcatcgtct tagaacttac cgccaaagct gaagtggaac ttgccgccat
     1921 cctggcctac gccgtaatcc aggcggatag gcccgatcgg cgtggtgatg cggaagccga
     1981 taccaccgga ataatggagt ttattgtttt catggtacca ggggatgttt tctacgccac
     2041 cccaggcatt gcccacgtcg gtaaagacga cgccctggat cttcttggca atgggatagc
     2101 ggtattcgac ggtcgcttcg tacatcttgt tgccgcggaa ttcatcgtct tcatagccgc
     2161 gcaggttgtc ggcaccgccg agggtgaaca ggtcgttgta gggcatatca ccggtggcga
     2221 taccacccat gaggcggacg gcgatgacgt gggcccggcc aactttatag tacaggcggt
     2281 tttcggcgat gaatttgaag tagtcgaagt cgccgcccat gccgtggcca gcccaggtgc
     2341 ccgtaaagga caggcgcttg cctttcgtcg ggtcgtagac gttgtcgcgg ttatcgaaga
     2401 cgtgggacca ggtcaggcta ttggtgcggc cgaagttctt acccaagtaa tccattcccg
     2461 caaaatcata tcctttatta tcagggtctt ttgcatcgtt tctatagtta aacccgctat
     2521 cgtgatcggt gtatttcgtc cgctttgttt ccaaagtgac gtagtcgctg acgtattcgc
     2581 tgcggacacg gccgtacgtg acgttgaagc cattggtccg gcggtcatat tcagcgacgg
     2641 aattgccctt ttcatcataa tcgtcgtatt ccgattcgcg gtcgaagatg ctgaagccga
     2701 tggagtcgcc ggcatcgttg aggtacggat gggtatacga gaagatgtag ttcttgttgc
     2761 tatccgtgtt gccgccgaat tcccagctga tattggcctt atcgcctgta ccacgcaggt
     2821 tcgtttcagc caggccgagg atgccgacca ggccgtcgga gtcagagtaa ccggcaccga
     2881 tggtgaccga accggtcttc tgttcggtga cgtcgatttc aacgatgacg tctttgggat
     2941 tgcgctggcc cggcaggagg cggacgttga cgtcttcaaa atagcccgtg ttgtaaacac
     3001 gttggatact gcggctggca gcgttcttgt tgaagatatc gcccttcttc atgcgcagtt
     3061 cacgcatgat gaccttattc ttggtcttgg tattgccgcg gagattgata ttttcgatgc
     3121 gcggttcaga aatggcaatg tgcaaaacgc cttcgtcgtc gaggcggaca tcggagacgc
     3181 ggctcatcat ataaccggca ttggcaaagg ccgtgttgat attggctacg tcgtggctga
     3241 cgacggcggt gttgagcgtc gtgcccggcg tcgtagcgac gagtttcatc aaatcacctg
     3301 tactgaggga ttcgttgccg acgatgtcga ttttcttgac gaccggattc atctggaccg
     3361 tatagttcag ggaaacgcct tccggtacag tcgtaaagga cggcttgacc gtcgagaaca
     3421 ggcccgtcgc cccgatgacg ttgaggtcgt gacggatgta gtccatgttg acggcatcgc
     3481 cgacgcgcat ggccagctgc ggtaggagtt tctgttcaat ctgggcttcc ggtacggggc
     3541 cgaccgtaac ggaagtggct accttgtcga catagggttt caagtcggca tcggtaacgg
     3601 cttccccata aggcaggacc acgtcctggc cggcctggat gacggccagg ggatagccgg
     3661 ggatggacgt atcaaactgc atgtcgctgg taccgctcat gtagctgact ttgtcgcccg
     3721 taatctgcgg cgtcgtatcg acaggagccg ggttgaggat ttcattttcc ttgtggacgg
     3781 cttctgctgt ttcctgctga actgcagccg gactcttacc agccgcaatg gcggccagtt
     3841 cttctgccgt ttcatagccc gtcaggcccg atttagccgc tttcatggca tcggctgtcg
     3901 tttcatcatt gggcgaggtt acgaccgtat tgacagctgt tttctggtca tccgccggag
     3961 cagctgccga gccagatgta accgtctggc tgcccgacga ctcataacct gttgccgctg
     4021 tcgtatcatc agcccaaaca gggcacgtca tcatcgcact ggttaccatc agggcgagaa
     4081 gttttttctt ccgcatattc ataaaaatcc tccattatct atttcgtaat acctgtaccg
     4141 cgcaaggtat gacgtgcttc ttgtacagta gcttgtatct ggcggtccga caagactgcc
     4201 ttccttgcca cccgtaccgg agccgctgct ggcggttccg gtacggctgc agccgtcgcg
     4261 gctggctgca caggcggttc gtctccggca ggagctgctg gcggcgcaga tgctgccacc
     4321 ggtgctggtc cttcctggac agggggctgc acgctgcctg ccgggaccgg ctcggccaca
     4381 ggcgcagccg tatccaccgg gtcccggtcc gcttcattcc ctgccgccgt ctcctggacg
     4441 acggcctcga cggccggtgg ccgttcttcc ggcccggtca ggcggaaggc ataccagccg
     4501 cccagcgcca ggaagaagga cgtgaccatg gcaccggccc gcagccagtt cctgctgact
     4561 ggatggtgtt tgcgcaaatt cttcatttcc gcttcggcca gcatcagatt gagttcgccc
     4621 ttgatatcgg actcttcatc aaacgaggct tcggcctttt ccagccagga ccggacggcc
     4681 cggacccggt gaactgtatc gcgttgggaa tgtttcatac agccttctcc tttctgccta
     4741 gacgaatact cttataaaat cggcaaaggc cgtatgaatt tataccggca aaaaggcgca
     4801 aaaacatgcc ataatctata ttttacagca tttttcgatt attttcacct aaaaacgggt
     4861 caattttaag aaaatgtaaa aaaattttag tggcggctct catggatgaa ccggctgagc
     4921 atgccgcgca cgcggcggat gccccgtttc tgcaggcggt agacgtaagg caggctgacg
     4981 tccaggctct tggccaggtg cttctgctcc acgtcgttca ggtacacgcc ttcgacgacg
     5041 gcctgttcct tttcgggcag ccttttcaag acgtggccga ccttttccag caacagctgg
     5101 cggctcgtgg cttcgacagg gtcctggcgc tcatcgcaga gcgtatcctg caacgtcacg
     5161 ccccgttcat ccggttcgtc aagggatacg gcgctgacgg cgccttcgcg cttcagatag
     5221 tccaggatgg ccccgcggat gcggtggacg gcaaagagcg ggaaggccac gccacggcgg
     5281 taatcatagc gttccacggc ttcgatgagg cccagtgtcc cttcctgcag ggcgtcggac
     5341 aggatgtcgc gatggatgtg ccagcgcatg gcctctttga agaccaaggg ctggtactgc
     5401 tcgatgaggc ggctgcgggc ctccagattc ccctcgtcct tataggcctg ccacagggcc
     5461 cgttcttcgt cgggctggag cagggtgatg tgctgtagtt ctttcagata atcgtctaac
     5521 atggcctttc accacctttt cattcaaatg gaattcacac atatataggg acgggcgccc
     5581 ctacaaaggc aatggcgtat ccacacatat acagggatgg gcctgtgtcc catctgtagg
     5641 ggcttgcacg tggcaagccc gcttcaggac atgtttcctt cccgttattc ctgtatgtat
     5701 ggcgtcctgc catcaatccc cggatgcaac ggcatgaccc catatacgga atcaaaacga
     5761 atacttatag acaccgccga tgaaactgtc gtcgtcggac gtccaggcgt tgacgctgaa
     5821 cttgctgccc aggtcataca tgaagccgaa gcggtcgtcg ccgtggttga tgccgaaggc
     5881 cgccgtgacc atgaagtcgt tgaacaggta tttccccatt tcaatgttgt aatagttgcg
     5941 gcccacatcg gtattcttat cattgaggtt cagcgagccg ttggtgacgg tgagcatatc
     6001 cagagacaag gccttttcca ggctctgtgt aaggcccagg ctgttcaggg tcatgcggat
     6061 gcccgatccg aggaggctgt ccatatcttc ctcgctgagg gagctctggg ggcggccgcc
     6121 gctgttgcgc agggtaatga gcgatacgat ctgctgcttc gtcaggggcg ggtcggagcg
     6181 gagcatgagg tccatgttgt ccgcctgacc gcgcagggtc agcatgacat tgtactggcc
     6241 gacgcggctg aagccttcag cgtcgacata gggcaggaag gaatcatacc gtgagaaatc
     6301 ggccttagcc ttggtcacgc ggaaattggt gtccaaataa tggacggtcc cccgcgtcgc
     6361 ttcaaagcgg cccgacggat ggggatgatt gaggttgccc tggaaggtga ccgatccatt
     6421 gaccatcaag tcgtacagag ccagattgta cagacggacc ttgttgccca gggtcagggt
     6481 gaaatccagg tccaaatcgg gcccttcact gctgctggcc agagccaggg gaatgtcgac
     6541 agtcgtattg tcgacctgca tgaggccgct cactttaggc cgcccgtcgc caggcgtgac
     6601 gttgacatag cccgtgatgg gaccgtcgta ataggggcag ttcagataga ggccggaaaa
     6661 atcagccgcc agggaatacg aatctaagct gcggccactc caggcggctt tgccgtcaag
     6721 gctgatgctg ccgggattct tcttcccttt cttatccata gtaccgctgc cgctgaggac
     6781 ggccgaacgg cctttgaagg ccaggtcagc attgatgttg gccaggggat aagccgtgtc
     6841 cttgaacttg atggtgccgt tcttgacggc aatatcgccg ttgaccgtcg gtgcatccag
     6901 ggttccgccg attttgacgg ccccctggat gggaccgtcg gcttcggtga cgtaaggcgt
     6961 caggaaagtc aggacatcga ggccggcgtt atcgagctgc agcgaaagtt ccatggattc
     7021 gccggtcttg ccgccagaca aggcgctgac gggaatggta ccgtgtgcgc tgaccttata
     7081 ggggtcgcgg ctgccgctgg cctgctgcaa agtaatcagg ccgttgcgga tgttgaacag
     7141 ggcataagcg cccgtaaagg aaatgccgtt ggccgtgccg cttcccagct gggccgatac
     7201 gtcggcctgg aggtcatcgc ctgtaccgct caggtcagcg gcaaagtcga taggcgcatc
     7261 gacgacgaag tctttatgtc ccagaacgtc ggtcagggcc ttggccggga actgcttggc
     7321 agcgacggac aatttcaccg gaccgtgcag ggcatacgtc ccctgggcag ccaggacgcc
     7381 gctgccgatg ttcagggcca tcttgttgat ggtgacgacg ccattttcca tctggacgtc
     7441 gatgtcagcc ggttcgacgg cctggccgcc gaaagaagtg tcactgatgg tccccttcat
     7501 ggagaccgtc gggttgtcac ctgtgccgct gatgtcgacc tggccattca gcttgccggc
     7561 aatatcctgt actggcaggg ccgcgacctt gagcagaccg gcaatgtcgc cgttgacgac
     7621 cgtcgcctgg ccccgcagga gagacgattt cgtattatac aataaattgc cgtcatacgt
     7681 cccgccgagc tgggcgaaat ggaggtccgt caggcgcagc atattgtcat aataggcaaa
     7741 atcgccgtgg atattgttca gggccatgtg gttgatgacc agattggccg ccgtcagtga
     7801 accggctgcc gtcggattgt ccaggctgcc gccgatatgg gcctggacgt cgaaagatcc
     7861 gctgcgcggc gtcttgttct gcggtaccag gtgggaaata tcgacgttct tcccgatcaa
     7921 atcgagattg agcgtatcgc cgatggaacc ggacagggcc agggatgccg tgtaagaaga
     7981 aatatcgcca ttcgagagat agactgtccc attatccagg cggtaatcgg cgctgacatt
     8041 cttatagagg tagcctgcat aggatccgct ggtcaggcgg aacgagccgg aagccgtcgg
     8101 attatccagg ctgccgctga gggtaatcgt attatcggcc cagccagtga gcggcaggtc
     8161 tgccttgccg atagccggca ggagctgttc gatgcgcata tgcgccgtcg tcagcgatgc
     8221 ctgtatcgtc cgggcatcca ggtccgcctg gccgtgcagg gtgtgggtcc catcaccgcg
     8281 gcgccagtag agggccggga tttcgatgcg gctgccctgt ccccgggccg tcccgtcgat
     8341 gctgtcgaag gggacgccct tgatctggcc gctgcgggca ttaaaggcca catcccactg
     8401 ctgcccgtcg atgtggccgg acaaatcggc cgtcccatcc atgggaaggg acacgaaagg
     8461 cgaagcggcg tcgaggggca ggtcatggcc ggtaaaggac aggtgcggcg tctggctgtc
     8521 cacttcataa gaaccgctga cgtcgatact tcctcctgcc aggtcagcat gaccgctgcg
     8581 caaggtcatg acgcgaccgt cataggccac atcgccggct gccgagtcga cagtcaggcc
     8641 attataagac aggtcccctg ccgtcacgtg gcctgtcgcc ataatcgagt tatccttact
     8701 gttgccggct gcgtggagcg acgccgacag actccccatg acggccacag gcacgccggg
     8761 cacggccgag aggtccagtc catctacgtc ggcatcggct tcataggcac cgctctggat
     8821 gtcatagacg cccttcccgc tcagggaagc gccataggcc tgggccgtca gtgaatcgat
     8881 atcgagccgg ttctgactgt aaatcaaatc tgcctgggct ttatcgacgg tatagccgtt
     8941 ataagtcata tcgtgcagag acgctgtacc gcgggcattc acgtcctgtg ccgtccccca
     9001 gaccgtccct ttgaaaccgg ctgtcccgct gatagcaacg ggcagataat cggcaaaggc
     9061 cgtcacgtcg gctcccggga cgtcgacgtt caggttgaat accggcgtgt cgccgttggt
     9121 tacaatagtc ccgccgacgc ggaagtcctg gccgttgaca cggccttcga cgtccgataa
     9181 ggtaatatct ttgctcgtca gggccgcatg gcctttcagg ccgtctacct cgaggccctg
     9241 gacggtgacg tagccgtcag atacgtcggc ctggccggac aggaaaaagc cgttgtggct
     9301 gtccgcgacg cgtactttga cgttttcgac ggtcccgctg cggatgacga catcggccga
     9361 cgacgggatg aatgacgacg cataagtccc gttgacggcg tcggcgctga cggtgaaatc
     9421 atactgccgg ctcgaggcat accttccgtg agccgtcacc ttttgcccat cgacggtcat
     9481 ggacgcatcg atggccagcg acggatagtc ggcaaaggcc acgctgccgt tgacattttc
     9541 tccggtgacg accgtgctgt ccggtaattg ggcgtcgata gtcccgttgt gaacattgat
     9601 ggccccgcgg aaaccggcat cggtcgtact ctgattcttc ttgatgagcg acgccacatt
     9661 ccacgtgcca tcgtcttcat tctgccaaag atgcaggacg gggtcgtcga catcggccgt
     9721 atccagggcc tgcaggattt cgccctgcct tagagccgtc atgatcttgc cggggttgac
     9781 ataaacccgg acggcatcgc cttcgatgac gacccgccct tgtgcatctt taatgactgg
     9841 cttggccatg accagctggc cgttcaggga tatatccagt gaagaaaacg acaaggtccc
     9901 gttcaattta ctgtttgcag ttgtcgtaat cgcctggcca gccatctggg tcagagccgg
     9961 tttttccatc cacagggcaa tccctgctga caggaaaaag gccgtgacgc cacaggcagc
    10021 catccatttt ttcttcataa tcgagctccg catacaaaaa aagagagaat tgcacgttac
    10081 aattctctct ttattttacc tatggagttg aggaaaatca agaaatagat gacaaatagg
    10141 atatttccct tgtaaattgt taaaaacctg tcatcatctc tttaagccct gtttatcccc
    10201 gatatgtcgt cgccgtcaaa gacggatgga tgacacccga ttcgacatcg acgcccatga
    10261 cctgttccag tttggcaatg ccgacgttgt agtcgtacag ggcctggatg tggttgacct
    10321 gggcttcgtt gaggctcagc tgggcatcga ggacgtccag gttgatgccg acgccggcct
    10381 ggtaacggat attctggata cgataatttt cactggcatg gtcgacgaca gtctgcgttt
    10441 cttcgacctt ctgggcggca ctgcggatgt tgaggtaggc ctgcttgact tccagctgga
    10501 cggcttcgcg ggtcttctgt tccgtttcac gggcgacgag gaggtcctgt ttggcctggt
    10561 tgactttagc cttcgtcgcg ccgccgtcat ataagctgta ggtgacgcca gcagctactt
    10621 tccaacttct cttattagca tcaatgcctg ggaaggtgtt gtcagcccaa tcattaccgc
    10681 ctgtaatggc gacagtcggc ctattgccgg aattgtcgag gtcgatgttg cgttccgcgt
    10741 cctgg
//
LOCUS       DAWCYS010000034        10710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_402_length_10710_cov_7.693383, whole genome shotgun sequence.
ACCESSION   DAWCYS010000034 DAWCYS010000000
VERSION     DAWCYS010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_10710_cov_7.693383"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2565 a         2515 c         2915 g         2715 t
ORIGIN      
        1 cgttcaaagc gtcagtacca gcgttcggtt cggtgaggcc gaaagcgccg agttttgtgc
       61 cttctgcgag gggtttgagg aatttagctt tctgttcagc tgtgccaaat ttccagatcg
      121 gccaggagca gagcatagtg tgtacttcaa agctgagtgc gatggagggg tcaactttag
      181 ctacttcttc gcaagcaact gcgaggctga ggaagtcagc acctacgccg ccgttttctt
      241 cttcccaagg aataccgaga aggccgagag tacccatttc gtcgaggata gcgcggtcaa
      301 agacttcttt ttcgtcgcga tctttgacag tgggagctaa ttttttttct gcaaaatctt
      361 ttgcgagatt tgcgatatct tgctgtaatt cagaaagttt gaattccatg attgagtttc
      421 ctcctaaaag attgtatata tggtttttat ttaaaaacgg cagagccgtc tttaaagcgt
      481 catgaacagc ccacagcccg cacaacgcgg gctgcaagct gccatactgt ctgccggagc
      541 aggcaattta tcctgtagca ggcacctttc ggagtcccgc ccagatggct gatggatcag
      601 gcgattctta gagaatttcc gagaaagcct ggatacgggt gcggagctgt tcgagcgacg
      661 gagattcgat gtcgaggtcg aggacggtgt aacggacacc ggctgcttcg aattccggtt
      721 tgtaaatcgg atagtcgaat tcttccggat cgcagaagcg catcatgcag atgacgacag
      781 cgtcagcatt gtatttcttg gtcatgtcga tgagcatctg gccacgcggt ttgtcttcgt
      841 tcaaagcgag cgggcagcca tcgaagtcct gccactgctg agcgagctgt tcgaggggat
      901 cgatgccgga cggtacgtct gtacggaact ggcgggattc ctgagcgagg tcgtcagcga
      961 cgacagcgat gttgaattcg ctgaagatgt cgaggaattc atccggttct gccatgatac
     1021 cggagaggat gacttttttg ccattccacg gctgtaccgg ttctttcttg acagcgtcga
     1081 taagttcgcg gaccaaagcg gtgtgttcag ctttgtccat gaaccagcgg gctttgatga
     1141 cgtcatggcg tttgaccgga gtgaagatct gcgggtactg agcagctacg tcgcagaatt
     1201 cacgcataac ctgacggttt tcgttgtata ctttgatagc ttcctggata gccaggtcgg
     1261 agatttttac gttgaggata cgttccaatt cagtgcggac atgttcgtat tcagctttga
     1321 ggaagtcgac agccgggcgg atcttacggt tctggggctg tgtgaagacg atgaccggag
     1381 ctttgccgtg ccatttctgg ctcatgcagc ggagggtatc gcagggaacg gagaagatga
     1441 cagcgtcgag gcagtcatac gtgccgttga gctggagttc catgtcagcc tgcatcaagg
     1501 aacaagcgaa cggaggaagg tatgtacgag ctgcggagat ctgcgggttc tgggagccga
     1561 acatgcctac cgggagatag cctgcagcgt atacgatttc ttccggagcg tagtaaggca
     1621 tcatgcctac gaggcctttg ccctgttttt tataattcaa aaccgtcttc tggggatggt
     1681 tggatacttc ctgtaatttg ctgataagtt cgtcgatctg actcattagt ggtttcctcc
     1741 ttcattggct tttttgtctg ccatcatttc tgccaaagct tctacgcggg tatcgaactg
     1801 tgcttccgag aagttacgag catcggtctg gtcgccgtcg aaaatgacat acggtacgcc
     1861 gaggttcttc tggacgcgct ggccaccttc gttgttgagg aggctcatgt tcttgcagga
     1921 acggttctga tgcatgatca agccgtcgca tttgccgtcg cggacaactt tttcaagaac
     1981 agcgccgcgc tgttcgaggc agcagttgat atatgtacgg gaataagctt ctgccatgga
     2041 ttcgaggtcg cccggttcgt aggaaacgtc ccacatgcca ggataagcgg aaccagtcat
     2101 gagagcgccc tgacctttga gttctttgaa ggtgtggccg agagcgatcc agacagcgat
     2161 accttcccag gtaacacggg atttttcgtt ttcaccgaaa gcccatttct tattagctac
     2221 tttttcgtcc aattctttga ggaatttgtt gaacgtgatt tccgagtagt tcaaggagcg
     2281 ggcagcaacg gcgaggccca tgtagttgaa gaggtcgaag ccgttgagcg gcgacggttt
     2341 gtactggaag tacgtagcga ttttgttcca ggcagcgatg gagcgctgtg tctgtttctg
     2401 tacttcgaag aatttgtcat agtcgaaggg acggccgcag aggtcttcga gctgtttgat
     2461 agcgtgtttg aattcgccga cgatgtactg tttagcgtgt ttcgtaacag ggaattcatg
     2521 gttgaacggt acgtcgatgt tgatgagagg tacgttcaat tctttagcca agttttcata
     2581 ccatttgagc aaggtattgc agatgttgtt gcaagtgagg acaacatccg gaaggggaat
     2641 cggagaagcc ggggagtttt tgaggacttc cggcgtttcg cctgtcagag cctgctgttt
     2701 gaggagttcc atgtagccca tgttgacgcg gcagtaggaa cagatgtcct ggttgtaacc
     2761 tttgttttca gcaacttcga gcatggccgg agcaccgtga cgggcaccga taccagctgc
     2821 gtgagtttcc ggatagacga tggcgatgtc catagccgtg cagaattccg gaggagcgac
     2881 agaagcggac cagcaaacga ggcggccttc ttttttcgct ttacgtgcgt cttcatcgac
     2941 tttcggcaag aagtaaccaa gggcttcctt ggagctcatg ctttcaatat ctactgtttt
     3001 ttcttcactc atgattttgt tctctccttt gacttatttc ttcattttat cataagcaat
     3061 caaagcagct ccgagagcac ccaccgcttg gggatgcaga gcgactctga caggaatacc
     3121 taattctttc gatacggcat cgacgacgcc gtgatcgcgg gagccaccgc cggtcatggt
     3181 caggtcttca ccgacaccga cgcggcgcac gagagcgcag gctttggcgg cgatggactg
     3241 atggacaccg gctacgatat cttctttcgg gacattcttg gaaagaaggg aaatgacttc
     3301 cgattcagca aaaaccgtgc aggtactgct gacggcagcg ggatgcttcg atttgaagta
     3361 ccagtccccc atttcagaca tggggatttc cagtaccttc gacatgacgt cgaggaaacg
     3421 gcccgtacca gcggcgcatt tgtcgttcat ggcaaactgc atgaccaggc cctggccatt
     3481 caatttgatg gacttgacgt cctgaccgcc gatatcgagg atggtcgtcg taccggggca
     3541 ttcaaagagg gcccctttgg catggcacgt gatttcgctg acttcgcctt tggcgcagtc
     3601 gacattgaaa cggccatagc ctgtggcgat gatgttcgcc atgtcttcaa tttttaagtt
     3661 ggtatctttg aagacttcgt ccaaaacgcg ttccggaccg gtcgaaccgg tgccgatttc
     3721 aacgacggca tgggcgacga tcttcttgcc atcttccagg atgactgcct tggaagaaga
     3781 agaaccaacg tcgattccga gagtatacac agttttcacc tttctttata aaaaatcaat
     3841 cattaaaagg gttgggattg ctgcgattga tgggagctac ggtctggaca tattcgacga
     3901 ggatgccggc gtccgtgctg ggacggcgga agttgacgat gatatcgtcc gttccccgga
     3961 cggctttctt ttcgagcatg caaccaggct tctggctttc cataatctga cgtacctttt
     4021 cgacatcatc cacttcaaag gcgatatgag cgatacctcc cctgccatga ttgaaatctt
     4081 tgaggacccc ttcacgggga ataatgaatt cgataggcgt actgttttca ccttttttag
     4141 tgaaaatgag atccgcatgg taggcgtcga cgaaaccgga ataatccact tccagaccat
     4201 tggtcttgat gaattcatgg gccttttcca aggtcggcaa gacgatgccg atatggtgca
     4261 ggcgcatggg atgccatgtt tcgttgagct tccctttttc cgggccgaga ttcttaacga
     4321 aaggattttc gccgcggccg gtgataggtg ctgtcgtctg aacatattca acgaggatac
     4381 cctggttggt tgtcgggcgg cggaagttga cgataatgtc gtccgtaccc tggacagctt
     4441 tcttttctaa catgcatccc ggacaatctg cttccatttc ctggcggaca gcttcgacat
     4501 cgtccacttc gaaggcgatg tgggcaatgc cgccgcggcc accattgaat tgggtaagca
     4561 caccggagtg cgggataatc atttcaatcg ggctggcaaa ttcaccaaac ttagtgaaaa
     4621 tgagatcagc ctggtaagca tcgacatagc cggcatagtc gatttcaagt ccattattct
     4681 gcatgaattc atgggctttt tctaaggtcg gcaggacaat gcctacgtgg tggagtcgca
     4741 taggtttcat tttttcaacc ccatcgggcc gtcctggaag aggcgggcat ccatgagttt
     4801 cggattatca atgatcggct tgaagtccat gtgagcgagg atatcttttt caatatcgat
     4861 gcccggtgcg acttcgatga gtttcaagcc ttctttggtc agttcaaata cgcagcgttc
     4921 cgtgatgtag agaacgtgtt tgccgttgcg ggctgcataa gaaccgttga aagtgatctg
     4981 gtcgacagct ttgatgaact tcttggcttt gccttcctgg aggatcttga ctttgccgtc
     5041 ttcgacagcg attttcaagc cgccagccgt gaaggtgccg cagaagtaaa cattcggtgt
     5101 ctgctgggaa atgttcggga aaccgccaca gccggcaacg ttagtaccga acttgctgac
     5161 gttgatgttg cccgagccat cgcactgggc caggccaagg taagcgatgt ccagaccgcc
     5221 gccatcgtag aagtcgaact gataggtgtg gtcgatgatg gcatcggcat tgcgggacga
     5281 accgaagcgg gcaccgccct ggggtacacc accgatagcg ccgccttcga cggtcagggt
     5341 aatggtatcg gcgatacctt cttcaccggc aacggaagca acgtattccg gagcaccgac
     5401 gccgaggttg acgacagcgt tttcggtcag ttccaaagcg ccgcggcggc cgatgatttt
     5461 cttagcactc atggggagag ctgcgtcggc agcgccttcg ggagcacgat gttcgccgct
     5521 gagggacgga tcgtattcgc agtcatacgt ctgctgatgg tcttccggag ctgctacgac
     5581 gacgtagtcg acatagatgc cagggatctt acccatgcgc gggtcgaggc tgccgtgagc
     5641 gacgacgtct ttgacctgga cgacgacttt accgccacag ttgtgaacgg cctgggcgac
     5701 ggaagtgctt tcgaaaggcc cgatttcttc atccatggtg atgttgcccg attcatcggc
     5761 ataagtgccg cggaggaaag ctacgttgac cgggaaggtc ggatagaaaa gctgttcgtg
     5821 accgtcgact tcgatcaatt tgaccaagtc ttctttggtt acgtcattga gcttgccgcc
     5881 gagctgacgg ggatcgagga aagtttccaa accgatatcc gtgaagacgc cgagtttgtg
     5941 gcctgccaaa gcgcggaacc aatgaaccag cgtgccctgc gagaagttgt atgcttcgat
     6001 tttgttttca acagccagtt taccgatagc cggtactgtc tgccagtgac cgatgatggc
     6061 gcgtttcaaa agacctgtgt gtgccaggtg ttctgcagca cggccatcgc gtttaccctg
     6121 ggaacctgcg tagatgtaag tcaggttctg cggcgtgttc gtatccaaga accgtttttc
     6181 caaggctttg gtcagggctt ccggatgggc actgctgaca aaaccaatag acgtaatcgt
     6241 gtcgttgtct tttacgagct gagctgcttg ttcagctgta atgatttcta cttttctcat
     6301 taccgtaact tcctcctgct atgtagagtg taccgacgct gtcgtcgcta ctgtcgtacg
     6361 cctttgtgaa aattttccaa aggtgaatta ctgactagaa tgatatcatg gaaaacccat
     6421 aacgtcaatg aataaatcgg gacaaattag tcacaaggaa tagaaaatct acaaatctat
     6481 tccactttag aattataaat tttgatattt ataattattt atcgaacaac taattccaag
     6541 cgaattacgg caataaaatg gcttatatat tgacataaat agattaattc atcacgtatg
     6601 atttttagtt taatcgatat tatatattaa aaagtcggaa acccctgtaa aacagccatg
     6661 accaaaaggt actgttaaaa accaagcatg ataattcgtt atagtatgtg aaaaacggta
     6721 cttattcttt ccttctagaa tagttcgtaa taggaagttt aggggctagg gattagtggt
     6781 taggggttcg tggctagtgg ttcgcctgaa tcatctaaaa aagctgccac acgaagccca
     6841 gatggcttta tacggcagcc ttttttttgc gctgtagggg tcgccacgtg ggcgacccgt
     6901 tggatatttg gtggcgctct ccctaaaatg aataatcact catattctaa gacaggccta
     6961 tcatgattgt atagacataa tagattggag tatattgaac gttttcacgc atatcatttg
     7021 tcatgtataa cgtctacatc acatttcagg cgggcttgcc acgtgcaagc ccctacagaa
     7081 aaagggactg tcgcatgtgc gacagtccct ttttccctac cctaccagga cctgtccgaa
     7141 gtagacgatg gcccctaaga ggatgatata tggtacatag actttcatga ttgcctgaag
     7201 gatttcgtta tccttgccgg tcagggagat ggccccgacg gcgatggcga tactctgcgg
     7261 cgaaatcatc ttgcctgcgc aggtaccggc cgcgttggca gcggccagcc aggctttatt
     7321 ggcgttgatg gcggcagcgg catcggtctg gaggttgccg aagaggacgt tactggacgt
     7381 accggaaccg gtgatgaacg tgccgacaga gccgatgagc ggtgcaatgg ccggatacat
     7441 catgcccgtc gcttcgacag ccgtgcgggc gatatctgac gtcatgccgc tgtagcccat
     7501 gactttagct gtagcgatga cggcgatgat ggtgatgatc gtattgcgca ggcctttgat
     7561 ggtgttcagc aagacgaaga acatttcgct aaaacgggcc gactgggcct taccactgat
     7621 gaaggctgcc aggaggatga ggatgcccgg cgtattgacc cagatgaagg tataaggtac
     7681 gccgccctga ccggtataga tgaggaccga cgatttaata gccatgagcg gtgcatggat
     7741 gaagggaacg agtttcgacg tcagcaataa gaagacgaag atcaagatga agggcaggca
     7801 agccagcaga ccaccgcgga gcggtacgtg agccggattg atgtccatgg catattccgg
     7861 atcgtcgata ggacggaaat gggaataaac gacgatgagg gccatgatga cgatagaagc
     7921 ccccatgaca gccagttccg gaccgacgaa ggccgagacg atgagttccg gcacagccag
     7981 gcccagggcc gagagcaggg taaccatgaa aacgcctttg agggccttca gcgagccgcc
     8041 gacgatggcg acgatgatga acgggcaaag gatgttgagc ggcaggagct gcaagctgat
     8101 atatgtcgac agctggaccg gatcgaggcc cgtcaggtta gccagggtgc tggcagggat
     8161 accgatggaa ccatacgttg tcggaacgga gttagcaacc aggcagacca ggatagcctt
     8221 gatggggtcg aagcccaggg caaccagcat cgacgccggg atagcgatag ccgtaccgaa
     8281 gccggccatg ccttccatga aggcgccgaa accccagccg atgagcaggg ccaggacgcg
     8341 gcggtccttg ctgaccgtcg tcagcatgtg cttgacgata tccatgctct tggtgtacaa
     8401 ggtcagatta tacgtaaaga tagcggcgat gatgaccagt aaaataggcc agcatgccag
     8461 ggccacgcct tccagggctg ccgtagcggc atccatgccc ggcatatgat accaggccag
     8521 ggcgacggca aaagaaatga tcaaggcaat gggacatgcc ttgaagctct gcatcttcag
     8581 gaagcccaaa gaaacgatga gccacaaaat aggtgacaat gccaataaaa ataccataat
     8641 acaaataccc tctttctttg ttcacttttg aacttattat atgctcaaaa actattaata
     8701 attaaataat taattcccga acaacatgat actattatat aatagaaaaa gggcataaat
     8761 caacagcttg acaatgctat taaagtatag ttcaagcctt agttagacga aaaacgcaag
     8821 attatttttc gattttggcg aatattctta tttcccgtct ttattctttt tttcacatac
     8881 tatggatagg gtacaaaaaa atcagctatt tcctaagaaa tagctaaaaa catgttcaaa
     8941 aagtaacgtt ttattgttca tttttttgta tataaattac gcgctgcgga taaggtatat
     9001 caataccgtt ggcatcgaag gtatccttta ccttggacat agtgtcgtaa tagacggggt
     9061 aataattttc cttggcgacc ttgggaaaag cggcgatgcg cacggagctt tcggcaaatt
     9121 cgcggatgcc gatttccatc gtgtcggcat tgaggacccg cctgtcttcc cggaatatct
     9181 gcttcagcag ggcaatggcc ttgtgatggt cgttctggta accgatgtcg aagaaaaaag
     9241 taatgtacct ttcctgctgg taagagctgt tggtcacctg ggacgaggtg atactggaat
     9301 tggggatgaa aatcgtcttg ttgtctttcg tcgtaatagc cgtattcatc atctgaatcc
     9361 ctttgacgct gccttctacg ccgccgacgg cgatccaatc tccggctttg aaaggcttga
     9421 agatcaggat caaaaggccg gaagcgaaat tcgacagatt gctctgcaag gccaggccga
     9481 cagccaggcc aaaggcgccg aacgcggcca ggaaggaatt cgtcgggata ccggccttat
     9541 tcagggccga caacaggacc aaggccatga tggcataata aatcagctgg ctgacaaagg
     9601 tgatgaccgt atggtcgtaa ttggcatggg tcatcatgcg gacagccagg ttcttgaaag
     9661 cccgggccac atagcggccg acgatgaaga tgacgatggc atagatgaca tcgggaccgt
     9721 ggttaagcag aaaatccttt aaatggtcca gcgtcgtgac gctctgcgtg acctggttct
     9781 ggacatcggc ctggacgacg ttagccgcct gctgcgctgt atcggccgta ttttcagcaa
     9841 tgttcatatt tcgcaccctc ctcttgtgtg tagttatatt atacccggga ttttgggtag
     9901 tggctagtgg ctaggggtta ggggttcgta ggggccgacg gcccggctct cctgctagga
     9961 gagctgtcag cgcagctgac tgagaggtta acctctccgg cccttcgggc tacctctcct
    10021 atgaggagag gcttccgacg gatgccttac gttacagtta ccgtcattcc ctcattatac
    10081 tagaagcggg ctcaccacgt gcgagcccct acaattgcgc agacaggcga taactaattc
    10141 acggcctgta gccttgcgca taaatgtatg caaaaaaagg gcttgttgca catgcaacaa
    10201 gcccttttaa gttgctagtg gttagcagtt agtgattaga aaatttaaat tccatttcta
    10261 cagactacaa actccatact gcaaactata aagagaaagg ggcctcgcat gatggcagaa
    10321 accttcgtgc gacagcccct cttttaagta tcaagtttgt agtttttagt ttgcagttat
    10381 tggctaaaaa tttaacctct ttctacaaac tcggcactac aaactttaaa ctatctttta
    10441 gccttcgatt tcggatacga caccggcacc tacggtacgg ccgccttcac ggatagcgaa
    10501 gcgcaggcct gcttcgatag caatcggtgt gatgagttcg acttccatct tgacgttatc
    10561 gccaggcatg cacatttctg tgccttccgg cagggtgatg acacccgtta cgtcagtcgt
    10621 gcggaagtag aactgcggac gatagccgtt gaagaacggc gtatgacggc cgccttcatc
    10681 tttggtcagg acatagacct gagccttgaa
//
LOCUS       DAWCYS010000035        10444 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_412_length_10444_cov_6.416979, whole genome shotgun sequence.
ACCESSION   DAWCYS010000035 DAWCYS010000000
VERSION     DAWCYS010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_10444_cov_6.416979"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2390 a         2669 c         3042 g         2343 t
ORIGIN      
        1 ctcttatcct ggccctggat ttcgcgttcc gcaccgttga tcatgaaggt aatcgtgcgg
       61 acaccactgt catcgggatc actgatgttg atgagagtga tgatgaggtc cttcccttct
      121 tcgatttcaa cgtggacttc ttcgttcttc ttcatgccga agaagaaggt cggtgtatcg
      181 aggacgctga catcgccgta tttcttgacc atttcgttga agtccttgaa gactttcgga
      241 tactggcagt aagcgttgat gtcttcgtct tcgtgtttgt aaccggcatc ggtcaatttc
      301 ttgcggatcg cttcgaaatc gacaggagcc aggctcttgc ccgggcgttc cgtaaggggt
      361 ttcttgcctt tcaggacgat cttctggagt ttttccggga agccctgata agggataccg
      421 atgcggcctt caaagaattc gacgacagac tgcgggaagt cgaggacgtc gcctttatcg
      481 tagacatcct gttccgtcag gttgttctgg accatgaaca aggtcatgtc gccgactact
      541 ttcgacgacg gcgtaacttt gatgatgtcg ccgaacatca tgctgaccgt gtggtacatc
      601 ttcttgattt cgttccagcg gtcgccgagg ccgacggctt tggcctgctg gcgcaggttg
      661 gagaactggc cgcccggcat ttcatgctgg tagacttccg tgttcggata cgcatcggct
      721 ttgtcgacgc ccttatagta cggacggaca gtagcccagt aacgggacat ttcttccatg
      781 gcatcgacgt cgaggtcggg ctggcggtcc ttgccgctca aagcgtagta caaggtgctc
      841 atgctcggct ggctcgtgcc gccggcaaag gcggaataag cgacgtcgac gatgtcgaca
      901 ccggcgtcga tggcgcggtc atacgtgcag atggcgttgc ccgaaccgtc gtgcgtatgg
      961 agatggatag gcaggtcgac ggcatctttc agggccgata ccaggcggta agcggcttcc
     1021 ggtttcaaga ggccggccat atccttgatg gcgatgatat tggcgccggc attctgcaat
     1081 tccttggcca tgttgacata gtatttcaga tcgtatttat cgcgggccgg gtcgaggata
     1141 tcgccagtgt agcagagggc gacttcggcg agcttgttgt tttcgcggac ggcctggatc
     1201 gtgccataca tgttgtccag gctgttgagg ctgtcgaaga cgcggaagac gtcgatgccg
     1261 ttcttggccg acaggtcgat gaagttcttg acgacgttgt cgggatagct tgtatagccg
     1321 acggcattgg caccgcggac gagcatctgc aagaggatgt tcggcgcttt cttgcgcatc
     1381 tggtgcaggc ggacccacgg gtcttcatag aggaagcggt aggctacgtc gaaggttgcg
     1441 ccgccccagc attcatagga gaagaggttc ggcaatttct tggaagccgc ttccaggacg
     1501 cggagcatat cgatggagcg gacgcgcgtc gcgaacaggg actgatgggc gtcacgcatg
     1561 gtcgtatcgg taaagaagac tttcttctgg tctttgatcc actgggagaa tttttccggt
     1621 cccatggcat cgaatttctg cttcgtccca tccggatacg ggccggcaac aggcgtcggc
     1681 agttccaggg cttcaaattc cggtttgtcc tgatggccgg cgtcggcata accgttgatg
     1741 gtcgtattgc caatgtattt gaggagcttc gtgccgcggt cctggacgac gggcaggttg
     1801 aacagttccg gatggtcgtc gatgaagttg acgttatacg taccggcgat gaattccgga
     1861 ctcttgagga cgttgatcaa gaagccgatg ttggtcttga cgccgcggat acggaattcc
     1921 ttgaggacgc ggagcatctt ttcagctgct tccttatggg tccggccata agtcgtcgtc
     1981 ttgaccagca gcgaatcgta gtacggcgtg atgatggcgc cggtgtaggc attgccgctg
     2041 tcgaggcgga cgccaaagcc gccgccgctg cggtatacca tgatcttgcc cgaatcaggc
     2101 atgaagttgt tcatcgggtc ttccgtcgtg atacggcact ggatggcgtc gcccaggcag
     2161 cggacgtcgt cctgggactt gatgccgatt tcggcgctgg agagggcgtt gccgtcgccg
     2221 actttgatct gggtctggac gatgtcaatg cccgtgatca tttccgtaac ggtatgttcg
     2281 acctgtacgc gcgggttgac ttcgatgaag tagaattcgc cgtccggcgt aaccaggaat
     2341 tcgaccgtcc cggcgttgac gtaatggacg ttcttcatca atttcagggc agcgttgcag
     2401 attttctgac gcagttcgac aggcagggcc gaagccggag ccgtttcgac gactttctga
     2461 tgacggcgct ggatggagca gtcccgttca aagagatgga tgacattgcc gtgttcgtcg
     2521 cccatgatct ggacttcgac gtgtttcgga ttgacaatgc atttttccaa ataaatttca
     2581 tcgttgccaa aagccagttt cgcttccgat ttcgcgcggt cgtaggcatc gcggagatca
     2641 gccatgcggt cgaccatacg cataccgcgg ccgccgccgc cgttgacagc tttcagcatg
     2701 atcgggaagc cgacctgttt agcgaatttt tcgacttctg cataattcat gaccgggccg
     2761 tcgctgccag ggatgtactg gataccggcc ttcttcgcct ggatacgggc attgatctta
     2821 tcgccgaaca tgatgaggtg ttcaacttta ggaccgatga agataatgcc ttcttcttca
     2881 cagcgtttgg ccaggtccgc attttcagac aagaagccgt agcccggatg gatggcatcg
     2941 acgtcatgtt catgagcaat gcggataata tcttcaatgt ctaaatatgc atctaccggt
     3001 tttttgcctt caccgacgag atacgattca tctgcacgga aacggtgcag ggataacgaa
     3061 tcttctttgg aataaatagc tacagtacgg attcccagtt cattacaagc ccggaaaaca
     3121 cgaatagcga tttccccgcg gttggcaact aaaaccgaac gaattttttt catgaaaata
     3181 ccctcctgct tagaaaaatt aagtcaagac cggtaaacgc acagatgtcc actcccatac
     3241 attcaccgct ttcttcatta taacaaaata agaaaacatt acaagggacg gcgcactttg
     3301 tctttataaa aatactgtat acaagtcatg tttctccgcc cttttccccg tttccccggc
     3361 ttctgccgtt tatcgccctc atgatgatga ttgttccttc atataagaca atcatgggaa
     3421 cggcaataca ggtctgcgta aacatatccg tcgtcggcga cacgacaccg gcaaagatga
     3481 aggccaggat aacgaagacg cggcgcttgc ccttcagcat ggccaccgtc accaggccca
     3541 ggcgggacag gaccagcagg accaaaggca gttcaaagcc cagggcaaag ggcaggagga
     3601 aagcgaaaaa aaaggataaa tatgccgaca aagaaaacat aggctgcaac gacaccgtgg
     3661 caaagtccat gaagaactgg accgctgccg gcaggacaaa gaaaaaggca aaggccgatc
     3721 cgccatagaa gagaagcagc gccgacggga tgaaccacaa ggccgtcttc acttccccgc
     3781 cgcgcagggc aggccgcagg aagcgccaga actcgaagca gtggatgggc agggtcccga
     3841 tgaaaccggc aaaggcggcg atctctatat aagaaaaaaa gacttctgcc ggattgagga
     3901 aatacaattt cccggcagga gccgaaatga cggcgataat atcttcagca tatatatagg
     3961 aaataatact gcccaccagc caggccacga ggcagacgat gatgcgccgc cgcaattcct
     4021 ccagatggaa agtaatagga gctcgttctt cagacatggg attttccttt caggtgtata
     4081 aaagggcttg tcgtatgacg acaagccctt ttaagttggt agtagttagt tgttagtggt
     4141 tagcggatgg ctttaaattt aaacctttct ctgcaaacta caaactctgt actacaaact
     4201 aatcatacat aatgtacgcg cgacgggtcg tagcggtctt cgcggggttt ttcttcggcc
     4261 gtagcctgag gatagcccat ggcgacgatg gcgaacgggg tcaggccctg gggaacgccg
     4321 agggaccggc ggacgaggac ttcgcgggcc ttatacgggg cgacgccgag ccatacggca
     4381 ccgaggtcga ggtgtactgc ttcgagcagg atgttttcga cagctgcgct gaggtcgagg
     4441 ttgatgaaag ccggaaaacg catgttttca tcgcgggaac agacgacgat ggccaaggaa
     4501 gcaccggcag cacagccggc ataaggactg cagtgggaca ggtcctggat gacccgtttg
     4561 tcgcggacga cgtagaattc ccagggctgc tggttcccag ccgaaggagc ggccatagcg
     4621 gcacgcagta actgttctat cttttcatct tctaccatgg tcggtaaaaa tttacggata
     4681 ctccgtctgg caaagatttc gttcatgtga atcgactccc tcctataatc atatatctat
     4741 ctcattataa catatataga tagaaagaaa aagaagttgt ataggcttcc atcacgaatg
     4801 atgaaaaaga ggcttgccgc tatgcgacaa gcctctttgc ttggtgccga gggagggact
     4861 tgaaccctca cggtgttacc cgcctgattt tgagtcaggt gcgtctgcca ttccgccacc
     4921 ccggcatatc tcttgtgccc ggcacatggt gtttcagtgc ttggcacaag aagaattata
     4981 ccataactaa cggaatcgtc aagtattttt tttgaaaatt tttacgactt aatggccagc
     5041 cggtactttt tcttccgaat ctttggctac gaccatttct tcttctttca aatgagcgaa
     5101 gtatttcttc gtttcggcta caacgacgcc agacaggccg atgagggcga tgaggttcgg
     5161 gatagccatg aggccgttga cgatatcagc caggacccag atgacgtcga gtttgatgaa
     5221 agcgccgcag gcgacgaggg cgatgaagat gatgcggtac ggcatgatac ctttgacgcc
     5281 gacgaggtat tcgacgcagc gttcaccgta gtagttccag ccgaggatgg tcgtgaaggc
     5341 gaacaaggtc aggccgacgc agaggatgat gctgccgtag gtcgggaaag ccatggtgaa
     5401 cgccgcattg gtcagggctg cgccgttgac atcgcccatc caggcaccgc tgacgaccag
     5461 gaccaaaccg gtcaaagtgc agatgatgat ggtgtcgatg aaggtaccgg tcatggaaat
     5521 gaggccctgt tcagccggcc atttcgtttt ggcagcagca gctacgatag gtgcactgcc
     5581 caggcccgat tcattggaga agacgccgcg ggcaacgccg ttgcgcatag caaccaatac
     5641 cgtggcaccg aggaagccgc cagctgcagc cgtcggtgtg aaggcgctga cgatgatctg
     5701 ttcaacagca gccgggatct ggtcggcaaa gacgacgagg aaagcgaccg tagaaacgac
     5761 atagatgaca gccatggccg ggacgatttt ggaagcgacg cgggcgatgg actggaggcc
     5821 gccgatggtg atggcagcga cgaggatagt caatacgatg gccgtgtatt cgacggggat
     5881 gctcgtcgtc aggcgcgtga tttcaacgat ggaattgacc tgcgcgaaag tcccgatgcc
     5941 gaagaaggcg acgaggacgc cgaagaaggc gaacaagaaa gccaagggct tatatttctt
     6001 gcccaggccg ttttcgatgt agtacatcgg gccgccggca atatcgccgt tcttgtcgac
     6061 cgtgcgatat ttgacggcca gacagccttc ggcgtatttc gtggccatgc cgacgaaagc
     6121 ggccatccac atccagaaga tagcgcccgg gccgccggca tggacggccg tagcgacacc
     6181 gacgatgttg cctgtcccga ccgtagcggc cagggccgtg cacagggcct tgaagctgtc
     6241 tacgtcgcct tcgccctggt tcttggccgt gaagatgagc ttcagcgctc tcggcaggcg
     6301 gaagacctgg agcaaatgga ggcggacagt cagcatcagg ccggtgccga tgagcagcac
     6361 cagcaagggc ggaccccaga caaaaccatc aatggcattc aacgtttcta acatctttca
     6421 tactcccctt ttgttatggt aattataacg acatgaaaaa agacctatca tcatcactct
     6481 gaagataggt cctaagggtc gcagaaaaag aaaggtcgtt ctgccagctc tgtccttttg
     6541 cctgagagat taggaatcgc ttccttgcac cttcggcgcc caaacgggac tctccagagt
     6601 tgtgtccaca cacggtccca cccctttgcg ggggcacctg agagtttgac tccgtcggta
     6661 ggcattgcct tctcccgtgt gtttcgttca cgcaatatgt atgatgtgat tgttattata
     6721 aaccggcttc ttaaaaaatg caagcccttt acggaaattt gtccgtattc agcgaacttt
     6781 ctttataaat ctgtgtcaga aaaggcgtac ggcaggagct gatccatcgt gacgacgcac
     6841 cattcccctt tcaggttggc catgatgacc ttggggatat ggaattcggc catgacctgc
     6901 cggcaggcgc cgcagggcga acaggggccg ggcgtatcgg cgacgatggc aatagccgta
     6961 aagtcccgct ccccttcgga tacggccttg aagatagccg tccgttcggc acagttgccc
     7021 aggggataac tggaattctc gatattgcag ccgccataga ccttccccga cgcggccagg
     7081 acggcggcgc cgacgggaaa atgagaatac gggctgtacg aatgagcgcg gtaggctttc
     7141 gctttttcta tgagttcttc atcagtcata gtgacagaca tccttttaag tggtccatga
     7201 cccggtcggc cgtctgctgc catttgagcg gcgtcagggc gatatgaccc tggcggatgc
     7261 aggcgacatc gctgtcgtcg ggagccgtcg tcatgtcgat atcgccgacg atacgatagc
     7321 agacgctgcc gtcgaggtcg cgtttctcag cgatgacatt gtggtaaatc tggaccgtct
     7381 ggggcacgac gcggacgttg tccaggctga cagccccttc tggcggcacg ttgatgttgg
     7441 cgatgcccgg gaagcggcct ttgacgacga gcttctggac caggtcccag acgaggtggg
     7501 ccgtctgagc gcagcgttca tcggtcatgc ccagcatgga tacggcaaag gccggcaggc
     7561 cgtaatacgg cccttccatg gccgccgaca cggtcccgga atagaggacg tcgctgccca
     7621 ggttatagcc gtcgttgatg ccggaaatga tgaggtccgg tttatcttct ttgaggaaat
     7681 attccatagc catcttgacg cagtccacag gcgtaccgga tacggcgatt tcccggattc
     7741 cctgtaccgt cgattcgtcg cggcagtaga gggccttgtt gatggtcatg gaatgagacg
     7801 ccgcactgcg ctggccattg ggcgcaacga cagtcaggcg gcagtcatga gatgccagtt
     7861 cccgttttag cgtctgaaga cccggcgctt tcaatccgtc atcattggtc aacaaaatat
     7921 gcatagtcta tctccttact tcttattttc tttacgcaat aaggccgcgc tttcataaga
     7981 cagggcccgc gtgtgcaggg tgtggctcca tttcatcttg tgccggtctt tgaaaccgag
     8041 gaagttgaca gggatccagc tgtacatgaa aatcggataa ataatgaggt atgcccatga
     8101 tttcggcggc accttgatct gcaagaggac gatgaccgga atgaggtact ggccgacgcc
     8161 gatgatggtc caaatctgga cgggcacgat ggcattgagg atattcgtat agaagggcag
     8221 gtacgaatta atcgaagcca ggacggcgta aatggtcgaa gccatcatga aaaatggctg
     8281 ggacaaggtg acgatgccgt cgagcatgcg gatgttgccg gtcttgatac cccgggcgaa
     8341 gagcttggga atgaagcgtt cgccgcagtc acactggccc tgggcccaac gcttgcgctg
     8401 gcggcaggac tgcatgaagc cgacgacctt ttcgtcgtaa atgatggcat catgggccca
     8461 gcaggtgcgg acgccttcga ggaggacttt catggaaaat tccatgtctt ccgtcaggca
     8521 gtcacagccc cagccgtact tgcggacgat ttccgtggcg atgcacatgc ccgtgccgcc
     8581 cagggccgta gacaagccga tgttgtactt ggccaggtgc cacatgtggt tgatgagcca
     8641 gaaggcgatg gaaaaggtgc cggcaatcca cgagtccgtc ggattcttgg aatccatata
     8701 gccctggatg accttttccc ccttgcagag gtggttgttc atttcccgca ggaattccgg
     8761 atggaccagg ttgtccgcat cgaagacgac gaaggcgtca tactgcttct cctgggccag
     8821 catctgcgag aacatccagt ccagggcata gcccttgccg acctgttcct tgttggtccg
     8881 caccttgacg atggccccgt gctcgcggca gatgtcggcc gtatggtccg tgcagttgtc
     8941 ggcgacgacg tagatgtcgt acatatcctt cggatagtgg agctgtttca gattgtcgat
     9001 gagctgccag acgacttttt cttcattatg cgcgcaaata acggcagcga aggtattctt
     9061 aggcgctacc gtaatgcgtt cccgccgctt catgaggccg aagcaggcga ggatgaaata
     9121 atagagggaa taaaaaacga tgatgacctg tatgggtatc atgatgatat ccaagatgtg
     9181 ttccatatgc gactctccct aaagacttag cgagcagctg cgacgttcat ggccgtattg
     9241 actaacccga agacgatata cagtaagaaa ggcatggtga tgatcagatg gaagtcgacg
     9301 aagaccagga ccagaacgcc gaagatgagg gaaatggcca gggccgattt gtggagcacg
     9361 tccgggctgg cccctttgaa atcggggtta tggaccttgc tgaccagatg gtagcccagg
     9421 aggacgacga agatggccag cagccatgcc ggcaggacgg cgccggaaat gacgtacgtc
     9481 gcaatgaggc agccgccatt aggaatcggc atcccctgga aatagccgtg gatgacgccg
     9541 gtgttgaggt tgaaccgggc caggcggata ccgccgagag cggcgtagat gatggccggg
     9601 atggcgccga tccagcccag ggactggagt gaataagaat agatcaggaa agccgaagcg
     9661 gccccaaagg atacgacgtc ggataaggag tcgagttcct tgccgaaatc accggcgaca
     9721 ccaagggcgc gggccgagcg gccgtcgcag gcgtcgcaga ccatggccag caggatgcag
     9781 acaccggccc aggtatagag cccgtggagg gtcataatca tactcaggac cccgaagctg
     9841 aggtttcctg acgtaaacaa acatggtatt acatttttca ttaaactatc ctccctatga
     9901 cggatttacc agcacggacc ttctggccct tcttgacggt gatttccaca ttgtcgcgga
     9961 catagatttc tgcacacgac ccgaatttga tcatgccgta gagctggccg tgctgcagtt
    10021 catcgcccaa ggtgacccag gagacgatgc gccgcgccag gatgccggca atgatggtga
    10081 ccaggatatc cgtatgggct gaatggatgc cgatggaata gcgttcattt tcatagccga
    10141 cgccttcttt ataagccgga cggaagcggc cgcacgtata ctgctggaaa tcaatggtcc
    10201 ccggcagcgg tgcccggttg acatgggcat cgaagatgga caggaaaatg atgattttcc
    10261 ggcacttctg gttgagatag gcatcttctt ccacttcttc gacgccgacg acagtcccgt
    10321 cagccggcga caccaggaga tggtcgtctg tcggcatggt ccggctgggg gtgctgaata
    10381 aataggtgaa atacgcatcc agattcagca gcggaaccag ggcgtaggca ttgatgaaat
    10441 agcc
//
LOCUS       DAWCYS010000036        10362 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_421_length_10362_cov_5.848258, whole genome shotgun sequence.
ACCESSION   DAWCYS010000036 DAWCYS010000000
VERSION     DAWCYS010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_10362_cov_5.848258"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9405..9414
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2083 a         3057 c         2970 g         2242 t
ORIGIN      
        1 gtccatcgtc ctggcggttc tcttctatgc cctcatcatc ttcggtgtcg gctatgtcat
       61 gaataattcg gaactcgccg ttgccatgaa aggcgtcggc ggcctggtca cggccgatgc
      121 catggctgtc gcttttggca gctccatcat ggccaaggtc ctcatcatcg gcggcctctg
      181 cggtatcatc accagctgga actccttcct catcggcggc agccgtgcca tgtattccat
      241 ggctgaatcg tacatgattc cgcacgtctt cgctaaattg cacgacaaat ataagacacc
      301 gatcaacgcg ctcctgctca tcggcctctt gtccatcatc gcgcccttct tcggcaagaa
      361 gatgctggtc tgggtcgtcg atgccggtaa cttcggctgc tgcgtcgcct actgcatggt
      421 cgccatttcc ttcgtcatcc tgcgcaaaaa ggctccggaa atggcccgtc cctacaaggt
      481 cagccattat aaactggtcg gcgtcatggc catcctcatg tcgggcttca tggtcgtcat
      541 gtacatgatc cccggctccg gcgctaccct ggttccccag gaatggggca tggtcctggg
      601 ctggtccgtc ctgggcgtcc tcttcggctt ctactgcaag gctaaatacg gcagtaaatt
      661 cgctacccac atcgacgtcg ccgtcgataa tgaagccgtc gaagaagcgg gcgacctggc
      721 tttcggtcag gccatggccg ctgccctgca cgacgtcgaa tccgatgcgc ctgtcaccta
      781 tgtatcgcag ccgctgagct tctccttctc catgcctgtg tccgtcgtct tcggtgccgg
      841 taagcgccat atggccgggg aactctttag ggcctatggg accaaggccc tggtcgtcac
      901 cgatgccgct accaatgggc gggacatcgt caatgaagtc gaaaagagcc tgaaagaggc
      961 tggcatttcc gtatcccgct ttgctcatat tacggggaaa tccctggata ttacggctga
     1021 ttcggcagtc gacgaaatca agaagaacgg cagcgacttc atcgtcgcca tcggcggcaa
     1081 taccgtcatg gaaggggcca agctgattgc cagtgccgct gcccgcacct tgggcagggc
     1141 catgatgccc ttgaatttcg taccgacgac gtatcacgcc ggcgcctgct tcgatgcatc
     1201 cctgcccgat ggcagccgcg acaacgccct gctggccaag gtcgtcctgg tcgatccgga
     1261 ctgcatgacc ggcatgagcc agaaagaccg ggctgtctcc ggcttcgccg ccctgtgcga
     1321 atgtatccgg gcctatacgg cgcccgatgc ccagccgctg actgatgcca tggccctcta
     1381 tgccatcgac atcctgaatg acaagctcgt cggcgtctgc ggggaaaaca cagacaaagg
     1441 ggcttgggaa aaagtcgtcc tggccggcgt catcggcagc atggtcagtg ccgaaacggg
     1501 ctgccaggtc gccaaagacc tgaaacaggc cgtcgccaag acgtcttttg cagccgatga
     1561 agtcctcctg gccgccatcg tgcccgtcgt cgtcgacgcc aaggccggta aggatccctt
     1621 cctctatggc aaactggccc gcaccatggg cggtttcacg tccgatgact gcgctgcccg
     1681 cctctgctac ctggccaagg ccctgcacct cgacctgcgc attgccgaca tgggcgtcca
     1741 tgaagacgac atcgccaacc tggccgaagc ctacatgggc gaagaagcgg ccgaagccgt
     1801 ccgggaaaaa gccgctgcta tggaagccgc atttgattcg tgaaaatagt ttgatatttg
     1861 acgaaaggct ctcctcatag gagagctggc cgccaaaggc ggtctgagag gttgatttct
     1921 cagcatttgt agtttttagc ccgccggata tatgtaagat acttgtgaaa ccgatttcgt
     1981 gaacgggcgc cccacgtggc gcccctacag aaaaaggcgc tgtcttcatg ggacagcgcc
     2041 tttttttaca tatacaaaag attttctttt tgccagcagg agtttgacaa ttctgtgtag
     2101 aatatcttct gtgtagaata tcttaagata aactagttat atagagttgc ttggaggtag
     2161 tcaatatgac actcagttta ttttggtcgc gttatacgct ggaacttcgg gaacagcatc
     2221 tgcgcgatgt cgatgaactt tatcattatc tcattgcctg ggaacgatgg aactggttcc
     2281 tggccaagat tccggaacag gcccagatcc agatactccg cggccacaac catggcgaag
     2341 attcctggac gtgccgcaag tggctcgacc acatgctgga ctggatcaag gagaacaagt
     2401 cgcctgccgt ctatgatgcc gtcatcgaca agattcatat gattgaagac aagccgattg
     2461 aagaactaga aaaacaggct gcccgcaaca tttcgccgga agagttggaa aaactccagc
     2521 gtgccggtta ttttcaatgt atcgaggcgc cgaacaaaag tgaatgaaag caggtgaggg
     2581 aatgaccttt ctattacgca tgataacagc tgctgtctta ttcttttccc tggcagctgc
     2641 cgcaccggct gctgtcgtgc aggccgattc cgtccgcgtc ctgcctattt ccggtgacat
     2701 cgacggcagc caggccgcct ttatccagcg cggcctgcgc caggccgccg aagaaggcga
     2761 tacggccgtc gtcattacca tcaataccct gggcggccgc gtcgacagcg cctggaagat
     2821 ccgcgacatg ctcatggcat ccgacgtgcc gactattgcc tatgtcacgt cgcgggcctg
     2881 gtcggccggg gccctcatcg ccctgtcgtg ccgccatatc atcatggcgc cgggcagcag
     2941 catcggcgct gccgaaccga ttcccaatac ggagaagaac attgccgccc tgaaagccga
     3001 gttcagcgcg acggcggccc agaatggcca taatcccggc gtcgccgaag ccatggtcta
     3061 caagtcccag ggctatcccg actatgccga accggggaag atactggcct tatccgacgg
     3121 ccaggccagg gaactcaacg tgtccgacgg ctcggccgat acgttgtccg aagccctggc
     3181 cctgtacagc ttgggcgatg cccgcctgac ctatgtcgac aaggactggc gtgatgacgc
     3241 cgtcggcatc ttgcagaatc cctatgtccg cacggtcttc gtcgccctga tcatcgccgc
     3301 cttgctggca gaaatcaaga tggccggcat cggcgccggt attgctacgg ccttcgtctt
     3361 cggcggcctg ctgctcctgt cgggcaatga gtccctggcc gacggcctga aaatcgtcgc
     3421 cgccttcctg gttagtctgc tctgcatcgg cctggaactg gcgtcgcctg gcgtcggcat
     3481 ctttggcctg gccggcgtcg tcctcttgtt cggcagcctg ttcttcatgc tgggagccac
     3541 gtatgaggcc gtctacatcc tggccggcgg gacggtcctg gccatcatat tgttctatat
     3601 cgtcggcaag catttgccca agagccggct ttttgaaaaa ttgaccctca agaaccgttc
     3661 gacgaaagaa aagggatata cggcccaggc cgattacagc aaatatctct atgaacgggg
     3721 caagaccatt accattctgc gtccgtccgg gacgatccgc atcggcaaag agcgcgtcga
     3781 tgccacggcc aacggcacgt tcatcggccg cgacgtcatc gtccgcgtgg tcaaagtcga
     3841 aggcagccgc gtcgtcgtcg aaccggaacc ggaacgccat cccaaacact gagaaggagg
     3901 tatcttatgt ctatcgtatc ctttttggca gtgcctgtca tcatcatcgt ggccgctgtc
     3961 atcatcgtat ccctgttcct gcacttcgtg cccatcggtt tgtggatttc ggccatggcc
     4021 gccggcgtat ccgtcggcat cgtcaacctc atcggcatgc gcctgcgccg ggtcatcccg
     4081 tcgaagatca tcctgcccct ggtcaaggcc aacaaggccg gcctcgatgt caacgtcaac
     4141 cagctggaag cccattacct ggccggcggc gacgtcgacc gcgtcgtcga tgccctcatt
     4201 gccgcccatc gggcgacgat gtccctgacc tttgaacgag cctgcgccat cgacctggct
     4261 ggccgcgacg tcctggaagc cgtccagatg agcgtcaatc ccaaggtcat cgaaacgccg
     4321 ttcatctcgg ctgtcgccca gaacggcatc gaactgaaag tccgggcccg ggtcacggtc
     4381 cgcgccaaca tcgaccgcct cgtcggcggt gccggtgaag cgacggtcat cgcccgcgtc
     4441 ggcgaaggca tcgtcaccag tgtcggctcg gcacgggacc attcgctggt cctggaaaac
     4501 cctgatgaaa tcagtaagac cgtcctcagc aagggcctcg acgccgggac ggcctttgaa
     4561 atcttgtcca tcgatattgc cgacgtcgac ataggccgca atatcggcgc ccgcctgcag
     4621 acggaccagg ccgaagcgga caagcagatc gcccaggcca aggccgaaga acggcgggcc
     4681 atggctgtgg ccaaagagca ggaaatgcag gcctataccc aggaaatgca ggccaaagtc
     4741 gtcgaagccc aggccgaagt cccgttggct ctggcccagg ccctgcgcga aggcaagtta
     4801 ggcgtcatgg actattataa tatgaagaat atcatggccg acacggacat gcgcgccgct
     4861 gtcgcccagg ccggcgtccc caagacccag tcggtcccga cggggcagga cagtcaccac
     4921 gacgaccgcg gtccggcagg ccatagtgaa aagtaggtga gccttatgga tatgttatgg
     4981 ctcatcgtgg ccttcgctct catggcgatc ctgccggacc tgctgcggcg tcgccgccgc
     5041 tatcccaggc ggaaagggcc gattcccgtg ccgccgcggc gtaaacccgc accggccaag
     5101 gcgccggctc cggctgaaac cggggaagat gcggctggga acgagatttc cgcacaggaa
     5161 acgacagctc cggcacagcc gcccatcgtg ccggcccgtc cggctatagc tcccgtatcg
     5221 gtgccgcacc gcgtccggcc cgcggcctgg agccagctga cgccgcaggc gcgggagctc
     5281 tacgccggct tcgtctggtc ggagatatgg caggaaccgc tggcttaccg ccggcgatta
     5341 attaaaaaat agagaggatt tatggaaatg attcaagaaa aacaagtagg tatcaagggt
     5401 atccccggtc tggtcattac gtccggtgaa aaagcgaaag gcgtcgtcct gttctatcat
     5461 ggctggtcgt cgcagataga attccaggcc gtccgcggcc gcatcctggc ttcctatggc
     5521 tacgacgtcg tcattcccga cgctgtcaac catggccacc gcggcacgct ggactatgaa
     5581 tcgaaagtcg tataccccga tttctggcag accatcttcc agaacctgac ggaagtaccg
     5641 gtcatgctgg aatatatcca ggaaaactgg cctgatacgc ccatcgccgt catgggccat
     5701 tccatgggcg gcttcacggc cttaggggct gtgagccttt tcagggaatt caagacggct
     5761 gtcgtcatga acggctccgg ctggtgggat gaatcggacc gccgcttccg ggcagccctg
     5821 cacatcgaaa agccgcgggc ctaccgtttc atacagatgc agttcgccgc catggatccc
     5881 tatacgcata tggaccgcct cagcggccgg tccctcttgt ccctccgcgg cggtgccgat
     5941 gatgtcgtcg acggggctgc ccaggaactc tatctggaaa aactggccca gcgcgatgac
     6001 gtcaagaccc agtccatcgt ctacgatgac ctgggccact tcgtcacgac caacatgatg
     6061 ggcgatgccg tcaactggct ccaaacagaa ctgcattaaa gagaaaatcg gatttgctta
     6121 aaaataataa gaggtaaaac ctttctctat gaaaagaggt aaaacctttc tctatgaaaa
     6181 gtattaaatt aattacggct gcatacttta caaatttaaa aatacgagta taatatacgt
     6241 ataaacagaa accgcaaagg tgcgaaaagc taagaatcag tcatctttat gatagaaatg
     6301 attcgttttc caccttggcg gtttttatgt ttttatagtt atcatttata atcaggggat
     6361 gtataacagg ggggataggt atgtcaaaga atattttccg aacgaaaagc atcaaccagt
     6421 tcctgtcgga aacgaaacag gacggcggca tgaaccgcgt cctggggact ttcggcctga
     6481 ccatgctggg catcggctgt atcgtcggca cagggatctt cgtcctgacc ggcattgccg
     6541 ccgccaattc ctccggcccg gccctggtca tttccttcat catcgccgca ctggcctgcg
     6601 gctgtgctgc actttgctat tctgaattcg ccgccatgat tcccgtagcc ggcagcgcct
     6661 atacgtacgg ctacgtagcc ttaggcgaat tttgggcctg ggtcatcggc tgggatttga
     6721 ttttggaata caccctggcc ttgagcgccg tgtccatcgg ctggtccggc tatttcggca
     6781 atatcctgac caatctggga ttggctctgc cgaaagaatt cattacggcg ccggaagaag
     6841 ggggcctcat caatctgccg gctatggcca tcatctggat catcaccttg atcaacatga
     6901 agggcatcac ccagagctcc ctggtcaatg acatcatcgt cgtcatcaag ctggctgtcg
     6961 tcggcctgtt catcgccctc ggcatcagcc acgtcgaacc ggccaactgg acgccgttca
     7021 tgccttatgg ctggtccggt gtcttcacag gcgcttcggt catcttcttt gcttacatcg
     7081 gcttcgacgc cgtctcgacg gctgctgaag aagtcaagaa cccgcagaaa gatttgcctc
     7141 ggggcatcat cttatcgctc atcatctgca cgattctcta cattgccgta tcggccatcc
     7201 tgaccggcat ggtcccgtac ttacagttca agacgacggc cgctcctgtt gcctatgcac
     7261 tgcagctcgt cggctatcat tggggtgccg ctgccgtctc cgtcggcgct atctgcggcc
     7321 tcacgtcggt ccttctggtc atgagcctcg gccagagccg tatcctcttc gtcatgtccc
     7381 gcgacggcct gctgccgcgc ttcttcggtc acatcaataa gacgaccaag acgccggtcc
     7441 gcagctcctt gttggttgcc atcgtgtcgt ccatcctggc cggcctcgta cctatcggca
     7501 tcgtcgctga aatggtcaac atcggcacgc tggcagcctt catcatcgta tcggcatcgg
     7561 tcatcgtcct ccgcaaaaaa gcgccggacc gcgttcgccc gttccgctgc ccctgcgtac
     7621 cgctggtacc gatcctgtcg atcctcttct gcggtatcct ggtcatcatg ctgccgacag
     7681 tcacgcagat ccgcttcatc gtatggctgg ccatcggcct ggtcatctac ttcggctatg
     7741 gctataagca cagcatcatc accaaagccc aaaaaggcac gctgaacgca gcccctgcgg
     7801 ccgctgctga tgaaacagcc agagtacctt cggtccacga aggcgctcac tccactattg
     7861 aacactgacg cattctatat atcgacagaa aaagacccgc cctctcccct ggccgggtct
     7921 ttttcttgcc gtcagctgcc agccgcccgc cctttgggcg tcccctacgc acaaggccgc
     7981 cggccgtgtg tcttttgcgg cctgccatct gcggactgcg gcctgcgctc atttccatcg
     8041 cctgcatgcg atttcgtagg ggccgtccca aagggcagcc cgtttcagga taggtgtggt
     8101 ttccccagct agcccgccag gtaccgacca gcgggagggg aagggggcca gaacggacag
     8161 ccgtggctgg cggcccgcag gcttcttgtt aaaaagcaac ctctcagtca gctgcgctga
     8221 cagctcccct agcaggggag ccgggccacg agccactagc cacgagccac taacccctaa
     8281 tctctcgcca gcagccgttc tttcatttcc aggtagcgtg tcgtgtagta cggtgccagc
     8341 tgggcttcgc gcaggtcgct gccgaagggg cggcggctga tggagaggat aggcgggctc
     8401 tggatgcgct tggcgacggc gaagccggcg atgaggcgcc accaccgttc caggtcgtcg
     8461 atgaaggctt ttgccgtcgg gaagagctga tggacatcga cgctgcagcc gatgttcttt
     8521 tccagggtgc cgttgatgta ccattcgagg atggttaccg gcgtggcccg ctgccagcgt
     8581 tcgacgaagg cggcgaagag gtagtcgtga taggggtcga tgatcggggc gccctgtccc
     8641 ttggtgatat cctgcttgtc cgacagttcg gcgctgggga cgatgtcgat gctgccctgg
     8701 ggcacgactt cgcgctggaa gacctggtca ttgaggtagc gggccaggtc gtagacctga
     8761 tgcttccaca ggtccgccgt agccgccagg tagccggcgc tgtcgccgta gagggtggcg
     8821 tagccgacgg acagttcggc cttgttggca ttgcacgtga agacgccgcc aaaggctgcc
     8881 gccgctgcgg ccaggatacg gctcgaccgg tcgcgggcct ggatgttttc ttcaatgaaa
     8941 ctggtcagtt ccagatgacc cttgtcctgg cctttctggc tgagcccggc tccttcgaac
     9001 tggtgccgcg tcagttccag gctgtcctgg acggggacga ccgtgtacag gcagccgatg
     9061 ttgtcggcca gttctttggc caggctcttg gtcatgtccg agttgtagca gctgggcatg
     9121 ttgaccagca ggatgttttc cggcggcagt accgtcgcat agagggcggc gttgacggcc
     9181 gagtcgatgc cgccggagac gccgaggacg actttatgga cgccgatggc atccatgaag
     9241 gatttcaggc cgtagtgcag gctggcgtag atgtcttcga tgggagactt ttccggctgc
     9301 aagtgcgaag gcgagacgaa acgctgggcc ttttcatcga agtcgacgat gaccatttct
     9361 tccgtaaaag gcgtacattc gacgtgctgc gaaccgtctt tctgnnnnnn nnnntctttc
     9421 tggtacgtgc tgctggcgcc gtcgaaggtg tagatgttct tgccgttgtt ctggataccc
     9481 gtgcagttga cgtagatggc cgggcagtgg accttgtcga tggcctggcc gaagaggcgg
     9541 tgacgccgct gggtcttgcc cagggtgaag ggcgagttgg aaatgttgat gaacatgtcg
     9601 atgtcgtaca ttttccctaa atagctcatg ggcttgaaag gatagttttc atcccagctg
     9661 tcttcgcaga tgagcggccc catgcggaac ttgcggccgt cgtcgaaggg aatctggacg
     9721 ggcgacagga agtcttccgg gaaggcatgg cgttcctggg cgacttcgat gagcgacgtg
     9781 aagtagcgga tgtcgctgaa ttcgcggtag ttgggcagga gcgtcttgat gtagaagggg
     9841 tagggcgagc gggccggcga aatccagcgg ccgttgtaag cgatgaacat ggtgttatac
     9901 ttccgcgtcc ggccgtcgag gttgacccgg tccgtctctt tggcgacgtt gccgaagatg
     9961 atggccatgc cgtcggacag ggccttgatt tcttcaccga agcggacgca gtcgtcgatg
    10021 tagtcgggct ggtcccaaat atcaccgatg agatagcccg gaatagcgag ctccgggaag
    10081 acgatgaggt cgcagccggc tgctttggcc ttggcgatac attctttcat gacggccgtg
    10141 ttctgccgtg gattgccagg gtggacattt gtctgggcaa gtgctatttt aaacaaggaa
    10201 aagcctcctt ttcgtagggg aaaaactatg tactatctca tcttatcata aaaaggggca
    10261 ggcgaaaagg ctgtccccgg ccaggggcgg aaaagacatg acagaacgcc gtccttgcgg
    10321 tagtcttaat acatataaaa gtttaacgag aggctttcat ga
//
LOCUS       DAWCYS010000037         9970 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_441_length_9970_cov_6.575088, whole genome shotgun sequence.
ACCESSION   DAWCYS010000037 DAWCYS010000000
VERSION     DAWCYS010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_9970_cov_6.575088"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2180 a         2913 c         2811 g         2066 t
ORIGIN      
        1 tagttggaag aacatagttc ggattatccg atattatcag ggatccgatt ttggagagct
       61 cgccgcgcgc cttatcgact tggcggacag cacggccaag ggccgccctt atgcggtcag
      121 tgagttcgta tctcccggcg ccgtccagat cggcgagacc atccaggccc acgtgccgtc
      181 cctggtcctc aagacctttg gcggctatga aggggcagaa cgggtcaagc tggctttcgt
      241 cagcgccgat tacgacgggc ctgtcgattt cggcatcacc gcctgccgcc tgtcctggga
      301 tgaccgctac cggctcctgg ggcaccgcga cgtcctgggc tcgctcatga gcctcggcgt
      361 cggccgcgaa cgcttcggcg acatcatcat gcaggatgcc ggcgccgtcc tcttggccga
      421 tacgaaactc gtcccgtatt tacagcagaa tttcactaaa attgccatgg tatccattgc
      481 catcgaagag atgcccctgt cggacattgc gcctcgccag gaaaaggtca aggaaatcaa
      541 gacgaccgtc gcatcgctcc gcctcgatgc cattgcatct tcgggctttg gcatttcccg
      601 taccaaggcg gccgaagcca tcaagggcga ccgggtccag gtcaactggc agcctgccaa
      661 gggaccgagc caggacgtca gtcagggcga tgtcatttcg ctgcgcggca agggccggat
      721 ggaactggcc gagatcacgg gtacgtccag gaagggccgt atcggcgtct tgctgaagcg
      781 gtatatgtaa ttccctttca cacggaggaa ataccatgct tacaccaatg gatattcaca
      841 acaaagaatt taaacgcggc ttccgcgggt acagcgaaga agacgtcgat gctttcatga
      901 acaacatcgc cggcgactac gaaaaggtat accgggaata ctgcgaattg aaggaacgct
      961 gcgacagcct gcaggacaag ctgacgcagt atgaaaagat ggaagctacc atgaacagta
     1021 cgctcatgct ggcccagcag acggcggaaa acgtcaaggt ctcggcccgc aaggaagccg
     1081 acctcatcct gcaggaagcg gaaagcaaga agaagcagat gctcgacgaa acgatgatga
     1141 atctgcagca gtcccggcag gaatgggaaa agctcaaggc ccagaccggg gcataccgcg
     1201 ccaagtgccg tgccatcctg accagccagc tcaagctgct cgacgacatg gccctcgatg
     1261 gcgaaggtgc ggcacctgtc gctgcggata aatcggtcgc cgtcaaaccg gcagccccgg
     1321 ctgtcgccgt ttcgaccgaa gccgcgcaga aagccgtacc cgatcatggc agccacgatg
     1381 acagtgatgc caaaaaggac gctaaaaaag cccaataata cgtattttat ggatatgctc
     1441 tctttcaggt atgacgcacg ttagacccgg cagggagtat atttgtttat agagggggac
     1501 aacgatgaaa cgattctttc ttttgctggc agcctggtgc tgcctggggc tcagcgcggc
     1561 cctggcctat aatccctatg cgccgaacca gtttgacgcc gtagaccgca atacctggga
     1621 atacaaggcc gtctacgacc tgtccaaggc cggcctgacg ggggtgccga tgacgcgctt
     1681 tgcgccgtcg tacaacctga cgcgctatga agtgacggaa atgatcgccg ctgccatgaa
     1741 gaaccgcagc aaagccacgg cagaccagca gcgggaaatc gataaactgg cccagagcta
     1801 tgccgacgac ctgcagtacg tcagcgacgc gccgaaggaa gccgaaccgt ccagtcaggg
     1861 cgtcgtcttc gactggaagg gggacaaggc ctgatgaagc cggtaaagat gatcaccatc
     1921 gacctcgatg ggacgctcct gcgcagcgac ggcagcgtgt cggaccggac ggtccgcacc
     1981 ttgcaggcag cccgcgataa gggcgtcgtc gtcgccatcg ccacgggacg catgtaccag
     2041 acggcccggc cttacggcga ccgcctggac ctcggcgaca gtccgctgct gctctttgcc
     2101 ggcggcctca tcgagacgct ggaatcgaag aagattctct tccagcaggc cattccgcag
     2161 cagacggcac aggaactggt cgacctggcc cgggaaaagg gctggcagat gcagacctat
     2221 atcgacgacg tcctgcgcac ggccaaggat gataagtgga tccgcggcta tgaagaaatc
     2281 acgcacagca aggcctgtat ctgcggcgac gaattctacc gcgtccaggg cgactgcaac
     2341 aagcttctca gccggggcga gcacgacgac ctggtggccc gcaaggccct catcgaggag
     2401 cgtttccccg gtcagttcaa catcctcttt tccatgccga cgttcctgga aatcatgccc
     2461 agcggcgtcg acaagggcaa gggtatccgc cgcctgggcg agctctacgg catcacggcc
     2521 gatgagatca tggccctcgg cgattcccag aacgatttgg atatgctcaa agccgccggt
     2581 ttccccgtgg ccatggccaa tgctgtcgac gaagtgaagg ccgtcgcgtc ttacgtcacg
     2641 gcgtccaaca atgacgacgg cgtcgccgcg gcggttgaga aatttgtatt gtaacattgt
     2701 ctatatagga ggttcttatg gaccattttc atttgatcat tgtcaccggc atgtccggtg
     2761 ccggtaagag ccatgctgtc caggtattgg aagacatcgg ctatttctgc atcgacaata
     2821 tcccgccggt cctgattccc aagttcgcgg aattgtgcat caaaggcggc gaacgggtcc
     2881 gccacgtggc cctcatcgcc gatatccggg gcggtgaatt ctttgacgcc atgagccagt
     2941 gcctgaaaga cctcaaggcc gaaggcattt cctacgaaat cgtcttcatc gaggcttcgg
     3001 atcaggccct ggtacggcgc tataaggaaa cgcgccgcgt ccatcccctg gccccgcatg
     3061 gccgcatcag ccagggcatc gccgaagaac gcaagcgcct ggcctcgttg cgccagaaag
     3121 cggatttcat catcgatacg tcgaatctca agccgaagca gctcaaggag atcctggtca
     3181 agcgctatgc cattacggac agcaagaaga gcatgaccgt taccgtcgtg tccttcggct
     3241 tcaagcacgg catgcccatc gatgcggaca tcgtcgaaga tgtgcgcttc ctgcccaatc
     3301 cttattatat tgaagaatac cgacataaat cgggccgtgt accctgtgtg cgcgactatg
     3361 tcgaaagctt ccccattacc cagacgtata aggaaaagtg gttcgatatg atcgacttcc
     3421 tcctgcccaa ctatgaacgg gaaggcaaga gccagctggt catcgccgtc ggctgtacgg
     3481 gcgggatgca ccgcagtgtc tgtatggccg aagccatgta taaacatctc agggataacg
     3541 gtgtcgacgt atccatcgaa caccgggata tccagaagaa cgacgtcgaa gaagatgcgc
     3601 cggggtacga aggcgaagcg taggaggtgc tgtcatgcgt tttatgaaat ggctgcatcc
     3661 gggcttgcac atcaagcgat ggctctttct cttcggcctg ggcatgatgt gctccagctt
     3721 cggcattatc ctgaccttta actaccaatg gctggggtcg ctggaagaat gggctttccg
     3781 cctgctctat gaagtgacgg gccattataa ctataccatc ctggccagta tgggcatcct
     3841 ggtcatcctc ctggggttgg tcgtcatggc ctgggcgacg cggcgcctga tccgtaccat
     3901 gatcggcgtc gtcatgcccg gcgaatcgga taacctcagc gacctgatct tttccaacct
     3961 ccagctgtcc aaagggccga aggtcgtcgt catcggcggc ggcacgggcc tgtcggtcat
     4021 gctgcgcggc ctcaaggcca agacatataa cctgacggct gtcgtcaccg tcgccgacga
     4081 tggcggttcg acaggccgca tccgccagga cctggatatc atcgccccag gggacctgcg
     4141 caactgcctg gtcgccctgg ccgataagga agggctcatg gaaaagctct tcgcccaccg
     4201 cttcggcggg tccggcaacc tgacgggcca cagcttcggc aacttgttca tcgccgccct
     4261 catcgaagtc ctgggcgacg tcgaggaagc catggacgcg accagcaagg tcctgcgcgt
     4321 ccgcggcaag gtcatcccat cgtcggcgga aaagcttcgc ctcaatgccg aaatgaccga
     4381 cggccgcatc gtcgaagggg aatcgcagat tccccatgcc cacggcaaga tcaagcgcgt
     4441 ctttacgacg ccggaacacc ccagggccat ccagtcggcc gtcgacgcca tcagggaagc
     4501 cgacgccatc gtcctcggac cgggcagcct ctatacgagc atcatgccca atctctgcgt
     4561 acccgatatc gtccaggccg tccgcaccag caaggcgccg aagatctata tctgcaacgt
     4621 catgacccag cccggggaaa cggatgatta caccgtatcc gaccacatcc gggccatcaa
     4681 ccgccaggcc ggcggcaagg tcatcgactt cgtcatcgcc aacaacggcg atgtcgatcc
     4741 ggccgtcctc cagcgctatg tcgcccacgg gtcccatccg gtcatgatcg acaagaagga
     4801 agtcagccag accggggcga cgctcatcct gtcgaatttg atcaacaagg aaaacagtgc
     4861 cactcacgat acgaagaagc tggccaacgt cctctttgat ctgatcaacg ccttgcggac
     4921 cgatttgtcg ccggagctct tgcattacta tttgaagcgt tacacattca accatcgtta
     4981 ggaggcggcg ccctttgtca tttgcagaag aagtgaagaa tgaaatcgcc cactgcaagc
     5041 ccgaaaatgc cgcctgccgt tttgccgaac tggcagcgct gctctgcatg ggcggctccc
     5101 tcatactcgg ctcgcgcggg actatcggca tcgaattcag cacgtccaat aatgccgtcg
     5161 cccgcaaggc cctgaagatg tggatggaat cgttcccggt ccggcccgaa gtccgggtcc
     5221 gccagggcct gcgcctgcgc aagaagaatt actatatcct gcgcgtatcg ccgtcgccgg
     5281 aaagcacggc catcctgaag gatttgtccc tgtttccgac agatacggaa ttttcggcca
     5341 tcgacctcaa gaagaccgac tgccagcggg ctttcctgcg gggagccttc atgggcggcg
     5401 ggtccgtcaa tcagcccaag ggcgattatc acctggaact ggtgacgggc aatgcggcct
     5461 ttgccgaaaa attgttgaaa atcatgcgcc gtttccgcct gccggccaag acgacggacc
     5521 gcaagggcga ttatttggtt tacatcaaag aaggcaatgg cgtgtcccaa ttcctgcaat
     5581 acatcggcgc agcccaagcg tatctcaagt ttgagagcgt ccgcgtcgtc aaggggatgc
     5641 gcaacaatgt caaccgcgtc gtcaactgtg aaacggccaa tctccagaag accgtagacg
     5701 ccgccgtgcg ccagcgccgg gccattacct gcatccagga atcgggcctg taccagcgca
     5761 tgtcgccgaa gctgcgggaa gcagcggaat tgcgcatggc caatcctgaa gcttccatga
     5821 gtgaactggc ggcccagtcg ggattgacga aatcgggctt gtcccaccgt tttaaaaaaa
     5881 tcgctgatat agcgaaacag atagaaggtc aacgagaaga tgaaatttaa gaaactatgt
     5941 acctatatag ccggcctgct gacggcctgt ctgcccctgg ccggcgccct ggcagcggat
     6001 ctgcccgctg tcgacgaccc ggacgtcatg gaccgcgtcg tcgcccagcg tatgcttcct
     6061 atttccatga cagctcctgt cgatacgggg acgctgctcc tctctgatag cccggaatac
     6121 gccgatggtg acgggatcct ctatggggat acggtcagcg gcgacgtgcg cctctatttc
     6181 tatcatgtca accagcacca gtataaccgg aaaatcgtag ccatgctcta caatccgcac
     6241 ccgtatccgc tggaagcggt cctccggggt tatcagtata cgcggcccag tacggactac
     6301 taccaggtcg gcaaggaact gtcgaccatg tattatgaag gcaacatgac ggttaataag
     6361 atcaccgtcc cggcccatga ctatgccatc gtcggtcagc gcctcaatga aactgtcgtc
     6421 cggccggacc agctcttttc gggcatcgtc aacgtcagcc tgccggaacc catgatcctg
     6481 tcgtccatga tcctgccgcc ccaggaagac ccgattgcct tcatccgcaa gcagcagtac
     6541 ctggccagtg actccgtcca gctgcgcggc actttccacg gcaaggaccg ttacctgagc
     6601 acgctcattc cctattcgac ggacagcggc atcggctata tcctcctggc tgacggcgtc
     6661 tgggaccgtt tcctccaggg ccgtgacgtc atggacaacc gggcctcgga agatacgggc
     6721 aactacggcg tcgactacac catccacctg cgcacgacgg ggaccgggaa catccacttg
     6781 tatttcaatc cccagggcgg cgaatatgcc ggcgtgacgg aactcatcta cagcgatccc
     6841 cagcggggcg aagacaagaa aatcgtcgaa ctgccgcgcc atcgccactc catgggcctc
     6901 aatgacccgt atgccatgga atacgtcgat accttcccgg ccgggacgga catgaccatc
     6961 cacatcatgc cgccgggagc cgccaacctg ccggtccgct tcctcgtcgt cccggacaat
     7021 aaactccagc ccatccgcga agccgtccag agcgaacgga ctgcaaaaga acgtcaggcc
     7081 gcagccgctg ccgaagcagc caaacgcaaa gccgcctata aagacaagac catccggccc
     7141 gaaacggaac ttcagtccga caaccagggc gagttcatgt ccttaaaaga ccgcctggcc
     7201 aaactccaga agaaggataa aaaatagtgg ttctcaaagc ctcccctcat aggggaggtg
     7261 gcccaacggg ccggagaggt tctttggctt tatagtttgt agtgcggagt ttgtagaaga
     7321 tggtttaaat ttaaaaccat ccgctaacca ctagccacta accactaacc acttaaaaag
     7381 gcttgtcgca tgtgcgacaa gccttttttt tactgcttcg gttcttcgcc gaaccatttc
     7441 ttatagattt tagcgtacgt tccgtctgcc ttgatttcct tcaaagcttc attgacttgt
     7501 ttgctcagtt ccggtttatc tttgctgatg cccatggcta cgccgcggga cgtaatgggt
     7561 tcgccgacga tcttgagctt atctttgccg ccctgctgga ggtaatactg ggcgacggct
     7621 ttatcgatgg aaacggcatc acacgtaccg gcttccagtt ccatccagcc ctgggtgttg
     7681 gtgtcgaatt ccttgacgtt ggccccgtgt tctctggcgt aatcggctgc cttggaaccg
     7741 acctgggcgc cgacggtctt gccggcgatg gagtcagaac cggtgaggtc cgtattgccc
     7801 gtcgggagga cgatggtgta gccaccctgg taatagacgt ccgtgaagtc gatctgtttt
     7861 ttccgttcat ctgtcgcatc catgccggcg gcgatgaggt cgatctggcc gctcttcagg
     7921 gccgggatga gggcgtcgaa gccgaggttc ttgacttcca tcttgtagcc cagcttatcg
     7981 cagacggcct gggccaggtc gatgtcgaag ccgacgtcct tttcattttc cgtgaattca
     8041 aaaggcggga acgtcggttc cgtgccgacg atgagggtct tgtcgccttt ggctgcgcca
     8101 tcttttttat cggctgtgcc gttggagccg cagccggcga gaagggtcag ggctgccagc
     8161 gaggcagcga cgagggtcgt aacttttttc ttcatcataa tgagttcctc caattaggga
     8221 atagtaagga tttcgctgag atccttcatg attgccacat ggggcaggcg ggcgaattcc
     8281 tggctcgtcg tcgttcccgt cgtcacggcg gcaaaggcga cgccggcatt tttggcggct
     8341 ctggcgtcga tggtattgtc accgatgtag aggacgtcct ttttatccag gccgatgtgg
     8401 cggcagacgg cgttgattcc gtccggtgcc ggtttggcct tggcgacgtc ttccacgccg
     8461 acgacgaggt cgatgaggct ttccatctgg tatttccgca gggtttcgcc gatacgcgac
     8521 cgggtcttgg tcgatgcgat gcagcacagg gcgccggcgt ctttcaaagc atggagcgtc
     8581 ggcagggtct gcggatagag atgggtgttg gccgtcatgt acttgtcgga gaagcggacc
     8641 ttgtattccc aaatcagggc gttgacccgg tccgggtccg tttccccggt caggagggcc
     8701 atggcgtcgt acatggtcat gccaatcgtg cggcggatag cttcttcagg gataccgtca
     8761 tagccttcgg cctcgaagag gagctggaag cacatgacga tacccttttc cgaattggcc
     8821 agggtgtagt cgaaatcgaa tacgtaggct ttatacatcg attatgcctt cggatcgacg
     8881 ccaaaccatt tcttgtacag tttggcataa gtcccgtcgg ctttgaggtc ggccagggct
     8941 ttgttgacct tttccaggag ttccggtttg tccttggaaa cagccatagc gataccggca
     9001 tcagctttct tggcgtcgcc gacgatcttc aggtctttat cgccgccttc tttcatgtag
     9061 tagagggcga ccgacgtgtt gatgacgacg gcatcacacg tgccgctctg cagttccatc
     9121 cagccctggc tgttggcgtc atactgcttg accgtagcgc cggcttccgt agccaggtct
     9181 acgcccttcg tgccgacctg ggaaccgacc gtgcggcctt tgaggtcatc gaagccgttg
     9241 atgttggtgt tatccttgcg gacgacgacg gaataaccat cctggaaata cggttccgta
     9301 aaggaaacct gtttctgccg ttccggcgta gcgtccatgc cggaagcaat catgtcgatc
     9361 tgaccgctgc gcagggccgg gatgagggcg tcaaaaccga ggctcttgac ttcaactttc
     9421 gtgcccattt tttcgccgat ggcattggcc aaatcaatgt cgaaaccgat gtatttgtcg
     9481 ttttccgtaa attcgaacgg cgggaacgtc ggttccatac cgacgacgag ggtcttctga
     9541 tctcccgaag cgccgtcctt tttgtctgcc gaagaaccac agccggcgac cagtgtcaca
     9601 gccgccatgg cagcggctgc caaagctaaa catttcttgt tcatgtgtaa caactcctgt
     9661 tcattgccta ataaggaaat ataaaataca actactcgac ataattatac tatatatagc
     9721 gcgaaaaata tatagcgcga aaaatatagc atatagtgca gaggggctct ttaacctctc
     9781 agaccgcctt tggcggccag ctcccctata aagggagcct tgggcggccc gcagcgaata
     9841 atggggaaga tacttgaacg gaatatgtgg catgggtttg cgaccggtga ccggcggcct
     9901 gcgtcaagga tttgaaaaaa atccttgaca atctctttac acagtagtat actgaataag
     9961 ctgtcggggc
//
LOCUS       DAWCYS010000038         9812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_450_length_9812_cov_6.495337, whole genome shotgun sequence.
ACCESSION   DAWCYS010000038 DAWCYS010000000
VERSION     DAWCYS010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_9812_cov_6.495337"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2065 a         2619 c         2797 g         2331 t
ORIGIN      
        1 aaggaaggcg atgtcggcct gtttcatgca ggcccggatg gcgtcgggat ccttgcgaac
       61 atcgtcggca atgggaacca attcgatatc ctggcgggta ctgagccgtt catgaatccg
      121 aaggcccgtc gtcccttcat gaccatcaat gaatactttc gttttcacac ttcatcactc
      181 ctatatcaca tgggctgtgc cctgactatt tattgaattt ttattcatta tagatgcatt
      241 ttaatatttt gtcaagaatt atgcacgatt ttttacataa ataatcgtaa ataaccgtag
      301 gggccgtccc aagggccttt ctggcccgtc aggtgccgac caacggaagg cgcaggacgg
      361 ttgccagaac ggacagcaga ggctgcggcc cgcctaacat caaccatcaa taaaggtaag
      421 cgatgcacct tccccgtcgg catcgaggcg gaccttgaca ccgtaaggca cgaaggcctt
      481 gtccttggta tggccgacgg gcagtccttt gatggcgggt ttcccggcca gatgggcata
      541 ataatccagg acctggtccg tcgtgaagtc gcccgggtcg ttggggcagt cggtgaaatc
      601 gccataggcg atggcggcga cgcgggacaa aaggccgctc tgccagagct ggttcagcat
      661 gcggtcaatg cggtaagcat cttcgccagt atcctccagg accaggatac agcccgtccc
      721 gtcgagggca taaggcgtgc cgacaaggga tgtgagaacc gtcagattgc cgccgacgat
      781 cggcccttcc gcctgtcccg gaacgacggt cgtcagcttc cgcccggccg gcagggccag
      841 aggacctcgc catgcagctg tcgtaaggcc ctggaagaac tgacctgccg tataggcatc
      901 ggaaccgacg agggtgacca tgagcggtcc attggctgtg actaagcggc atttttccca
      961 aagggcagtc tgcaaggccg tcacatcgct gaagccgacg accagtttgg gatgagacgc
     1021 gatatagtca taatccagct tatcgaggat acgcgccgag ccgtagccgc cattgaggca
     1081 gataatggcc ttgacatcat cgtcgtggaa caaggcattg atatcgcggg cccggtccgc
     1141 atcggtcccg gaaaagaaac cataattccc agtgaccgtc ggcgtcatct tgacgcgata
     1201 accggcccgt tccaaccggg ctacggccgc agaaatatcc tggccttcat cgtgcgtggc
     1261 cggcgctgcg atgccgatgc agtcaccgcg gcgcagtgcc ggtccccgaa gggccgttcc
     1321 cgccgttact cccccttgta gtaacgctcc atacccttgg catagccata gtcgtcattc
     1381 agatattggc ccgtcttctg cttgtaacag agattgacgg cgacgaaatc ggcaatctga
     1441 ttgtgcttga tttccttctt gcgcataggg atttcctgcc agctatgggt cgaatcatta
     1501 tacagccagg cctggccatg agccacctta taagtcaggg tatacgtccc ttcaatattc
     1561 cccgaatcct ctggcgaccg gaagagattg accttaaaaa tccagccctg accgtcatcc
     1621 ttcacggttt ccagcgaact caaatcgacg tacatttcat aacccatgcc aaaattaatc
     1681 ggccggtaat gactattcgc agccaaagga ctgttgaaaa aagcctgacc gctgccaaaa
     1741 cctccgaggg ccaccgcggc ccctaatgat agaatcatta acccacgttt catattcata
     1801 ccaaaaacct cccttcaaag tatgccttta ttatacgaca aaaaagaggc tgtcgcacgt
     1861 gcgacagcct ctttttgagt tttaagtttg tagtttttag tttgcagaaa atggtaaaaa
     1921 atttaaaacc agcttctaca aactacaaac tccatactac aaactataag ttattccata
     1981 tcataatcct gcaaggcagc gacgtggtga tagccttcgt ttttgccttt gttggcaaag
     2041 acgcggagga cttcacgggc tgtatcgagg gaggtcaggc agggaacggc gagttctacg
     2101 gccatgcggc ggaggttgaa gccgtcgcgt tccgggtgtt tgccataggt cagggtgttg
     2161 aggaccaagt cgattttgcc gttgcggatc agggtggcga tattctggcc gccttcgtgg
     2221 atcttgcgga cgatgtcgac gggcatgccg tggtcgttga agtatttgcc tgtcccttcg
     2281 gtggcgatga tgtgatagcc caattccttg aagccttcag ccagctggag ggcttcggct
     2341 ttatcgcggt cggcgacggt gaccaggatc gtgccgccag cggggatctt catcttagcg
     2401 ccattgacgg ctttgtacag ggcttcggag tacgaatggc cgatacccat gacttcgcca
     2461 gtcgatttca tttccggccc gagggcgatt tcagccaggc cgatcttact gaacgagaag
     2521 actggagcct tggcggcgac gtgtttcttg gccggcatga gaccgctgtg atagcccatg
     2581 tccttcaaag tttcgccgag ggcgatgcgg gttgcgattt cgacaatcgg gaaatgggtg
     2641 accttgctca ggaagggaac agtacggctc gaacgcgggt tgacttcgat gatgtacaac
     2701 ttgccgcggg aaacgacgaa ctggatgttg accgaacctt tgacctggag ttccagggaa
     2761 atgcgcgtcg tgtagtcgac aatctggttg atcatatcct ggtccaagtg ctgcggcgga
     2821 tagacggcga tggagtcacc ggaatggacg ccggcccgtt cgacctgttc catgatgccc
     2881 gggatgtaga cgtcagtacc gtcggcaacg gcgtcgactt cgacttcacg gccgaccatg
     2941 tattcgtcga tgaggatcgg gtggtccggt gaagcgatga cggcttcacg gagatagcgt
     3001 tccaattctt tttcgttata gacgatttcc atagcccggc cgccgaggac gtaagacggg
     3061 cggacgatga gcgggaattc caattccttg gaaaccttca tggcttcttt atcgctggta
     3121 gcgatgcggc cgtccggacg gggaatgttc aatttggcca ggacttcgtc gaagcgttcg
     3181 cggtcttcgg cgcggtccat gccgtcaaca gacgtaccga ggacggtaac gccccgtttg
     3241 gccagtggag ctgccaggtt gatagccgtc tggccgccga actggcagat gacgccgacc
     3301 ggtttttcct tgtcgacgac agccatgacg tcttcgacgg tgagcggttc aaagtacagg
     3361 ctgtccgacg tatcgaagtc ggtgctgacc gtttccgggt tgttgttgac gatgatgctg
     3421 ttcatgccgg ctttgcgcag ggcccaggcc gaatggaccg agcagtagtc gaattcgata
     3481 ccctgaccga tgcggatcgg gccggaaccg aggacgatga cgctcttctt gcccaggggc
     3541 ttgacttcgt cttcgtcggc atagcacgaa tagtaatacg gcgtagcggc ttcaaattcg
     3601 gcagcgcacg tatcgaccat cttgaaggtc ggcgtaatgc cgtggtcgag gcggaactgg
     3661 cggacttcgt cttccgtctt gttggtgaag ccggcgatga cggcatccgg catgctgtag
     3721 cgtttagcca ggcggatgtt atcgaccgtc aggttgttgc gcttcaagtc gaattccaag
     3781 tcgatgatgt tcttgatctt atggaggaac cacaggtcat agcccgtgat gtaatgaatc
     3841 ttttcttcgc tgatgccgag gcgcagggcc tgagcgacga cgaagagccg ttcatcatcc
     3901 tgtttctgca gaagttcgac gatgtcgatg agggacttgc tttcgagctt cttcatggaa
     3961 atatagttga cgccgatttc cagagagcgg acggctttga gcagggcccc ttcaaagcag
     4021 cggtcgaggg ccatgacttc gcccgtagcc ttcatctgcg tgcccagggt cttgtcggcg
     4081 agggcgaatt tttcaaaagg ccagcgcggg aatttgacga cgcaatagtc gagggtcggt
     4141 tcgaagcagg ccgtcgtctt gcccgtaacg gcattggtaa tctggtccag ggtcaggcct
     4201 gtagcgactt tggcggcaac tttggcgata ggatagcccg tcgctttgga agccagggcc
     4261 gacgaacggc tgacacgggg attgacttcg atgacgtaat actggttgct gttcgggtcc
     4321 agggcgtact ggacgttgca gccgccttcg atcttcaagc ggcggatgat gtccagcgat
     4381 gccgtacgca gcatctggta ctgcaagtcg ttcagggtct ggctcggtgc gacgacgatg
     4441 gagtcgcccg tatggatgcc gactgggtcg atgttttcca tgttgcagac gatgatgcag
     4501 ttgtcggcct tatcgcgcat gacttcgtat tcgatttcct tccagccggc aacggagcgt
     4561 tcgacgagga tctggttgat cgggctgagt ttgatgccgc gcgtggcgat catgaacaat
     4621 tcgtcttcgt cgtgggcgat gccgccgccg gtaccgccaa gggtaaaggc cggacggacg
     4681 atgatcggat agccgatttt gtcggcgaat tcgatggccg acgggacgtc ttcaaagata
     4741 tcgctttccg gaacaggctg gttgatatct ttcatggctt tcttgaagag tgcccggtct
     4801 tcggcttctt cgatagccga cagatgcgtc ccgaggagct ggacgttata tttttccagg
     4861 acaccggcgt tagccagttc gacagccata ttcaggccga cctggccgcc aagggtagcc
     4921 agcaggccgt caggccgttc tttttcaatg acttccgtca cgtagtcgag ggaaatcggt
     4981 tcgacataga cacggtcggc aatgtcttcg tcggtcatga tcgtcgccgg gttgctgttg
     5041 atgaggacga cttccagtcc ttcttccttc aaggcgcggc aggcctgagt gccggcatag
     5101 tcgaattcag cggcctgacc gatgataata gggcccgaac cgataacgag tactttcttc
     5161 aatccttcta tcatgttagt tttcctccat catcttgacg aattgtgtaa acagatacaa
     5221 attatcagtc ggtcccggcg aagcttccgg atggtactgg acgcacatga tcttcttgga
     5281 cgggacttca aaaccttcga gggtgttgtc gtggaggttg cgatgggtga tgacgacgtc
     5341 atcgggcaga gaggcttcgt cgatgacata gccgtggttc tgggacgtga tgtagacgcg
     5401 gcccgtgcgg atatccttga ccggatggtt ggcaccgcgg tggccgaagg tcagcttgcg
     5461 gctcttgccg cccagagcca ggccgagcat ctgaaggccc aggcagatgc cgaaaatcgg
     5521 tttcttgccg atcatcttct tgacttcttc gacgatgtac gggacgtctt tcgggtcgcc
     5581 agggccgttg ctcaggaaga taccgtccgg attgacggcc aggacttctt cagccttggt
     5641 ttcagccggg aaaatggtca ggcggcagtc gctgttgacc agttcgcgca ggatgttggc
     5701 tttggcgccg taatccatga cggcgacgtg gaagcggcca tcgcccatgt gcttgatttc
     5761 acgcgtcgtg acgcggcgga cgagctgcgt ttccagcggc gtatcgaaga gttccttgat
     5821 agcgctttct tccatcgttt ccggcacgat gacgcctttc atgacgccct ggctgcggat
     5881 ggcccgcgta atggcacgcg tatcgacgcc gtagaggcag gggatgtggt gtgtcataat
     5941 aaaggtagac agcttgcctt cgttctgcca gttgctcggt tcttcacaga gctcgctgac
     6001 gatgaagccg gcagcatagg gacggtcggc ctgttcgatt tcgtggaaca aaccgtagtt
     6061 accgatgagc gggtaggtca tggtaatgat ctgaccggca taggacggat cggtgaacgt
     6121 ttcctgatag cctgtcatgc ccgtattgaa gacaacttcg ccgacagctg gttcatccat
     6181 cagcatgttg ccataaaact tctgtccatt ctctaatatg aggacacctt tcattataaa
     6241 acctccccgt ttttcattac aatttcacca ttgacgatgg tagctgcggc cttgcctttg
     6301 aaaatcattt catcgaaagg cgacgctttt cccttagaat agaatttcga ggaatctacc
     6361 gtccattctt tatcgagatc gagtacggtc aaatcggcat ccttgcccgg tgcgatgaca
     6421 ccggcatcga ggccgaggat cttggcctgt gccgtcgaca tggcattgac cagagtcatg
     6481 aggtcgatat gacccgtatg atacatataa gtcagcatgg cgccgacgct cgtttccaag
     6541 ccgacgaagc cgctgggagc caggttgaat tcctggtcct tttcttccca ttcatgcggt
     6601 gcgtggtcgg tgacgacggc atcgatggtg ccatcctgca ggccggcgac gcaggctgcg
     6661 cggtgttctt ccgagcggat cggcgggttg actttatacc gtgtttcaaa agtgcgcagg
     6721 gcttcatcgg tcaggatgag gtgctgcggc gtgacttccg ccgtaacctg gacgccgcgc
     6781 ttcttggcct ggcggaccag ttcgacagcg ttcttggtgc tgatgtgagc gatgtggacc
     6841 ggcgtgtgtg tcgcttcggc caggaaaata tcgcgggcga cggcaatgtc ttcagcgaca
     6901 gccggacggc ctttgatgcc caattcgttg gaaacgacgc cttcatgcat gttgccgcct
     6961 tcgacgaggc tctgttcttc gcagtggtcg atgatgacct tgtggaacat gctggcatat
     7021 tccatggctt tgcgcatgaa atcgcagttt tcgacgtaac ggccgtcatc ggagaaagcg
     7081 gcgacgcctt ctttggccat ggcgcccata gcggccagtt cctggccctg gaggcccttg
     7141 gagatggcgc cgatgatttc gactttgact ttggcttccc gttcgatttt atacttcatg
     7201 ccgtcgacga tgattgccga atcgatgacc ggcttggtat tcggcatggt cgcaacgcgg
     7261 gtgatgccgc cggcagctgc tgccttcgtc cccgtttcaa tcgtttcctt gccggactgg
     7321 ccgggctggc ggagatggac gtgcatatcg atgaagcccg gtgctacgac gaggcccgat
     7381 gcgtcgaaga tttcggcgcc gtcagcgctc agattggctc cgacttcctt aattttacca
     7441 ttttcaatga gaatgtcaca aatttcatcc tgctgcatgg ccggatttac gactctgccg
     7501 cctttaacga gtagtgtcaa cgtcatcgcc tcccataatt gtcagatata atacggccat
     7561 acggacggat acgccgttct tgacctgttc ctgaatcgaa gaaaattcac tgtaacatgt
     7621 atccggttcg atttcgatgc cccggttcat gggacccgga tgaaggacga ggacgtcgtc
     7681 tttagcccac ttgaggcggt cgcggttgat accgaagagc gaattgtatt caccggcgct
     7741 gggtaagaag ccgatgccct ggcgttcttt ctggatgcgc aggacgttga tgacgtctgc
     7801 atcctggatg gcttccttca agtcgtagtg gactttgacg cccatggctt ccaattccgg
     7861 ctggagcagt gtccgcggac cgacgaggtg gacgtcagca cccatggtct tgaggccgta
     7921 aatattggag cgggcgacgc ggccgtggtc gatatcaccg acgatgacga ccttcaagcc
     7981 gtcgatatgg cctttgactt cctgaatggt gaacatgtcg aggaggccct gcgtcggatg
     8041 ttcgtgggcg ccatcaccgg cgttgatgat gatcgggtcg acgcagcggt cggcaaacca
     8101 aggcgaacct tcctgtttct gacgcatgac gatggcatcg acacccatgg ccgttaccgt
     8161 cagcagggta tcgcggaaac tttcgccttt ctggatggag cttccccgcg gcgtgatggt
     8221 gctgacatcg gcgccgagat acttggcagc catttcaaag gaaccgcgcg tacgcgtgct
     8281 cggttcccag aacatgttga ccaccgattt ccctttgagc agggataact tcttattccc
     8341 gctgttgacg acggctttca tcttcttagc tacctgcagt atcaattcaa tttcttcttt
     8401 cggtactccc tggagcccca acaggctttt gccctgtata ctaaccatcg aatcctcctt
     8461 gtaaaaaaag acctctgccg cagggcaaag gtcatattat aagcatggca aaatatgtcc
     8521 ctttttagcc tctccggact atattaaagg ttcaatctga aagtattaga atcgcacagc
     8581 ctcacgggac tgccacatat tcttctatat attgtatcgg ccagggccta taaagtcaag
     8641 cagttttttc acgacttcaa tccgtcgacg ccggcgtaat ggtcacggcc tggggcgtaa
     8701 tggtgacgct gttgtaaaag gcttcaaaga gctgggccgg gacgtacatc gtaccgttga
     8761 cattgaccgg gccggcaccg tactggaaga tgtggttcaa gttgaccttc ttgatcgtcc
     8821 cgatccgttc gtaccagtct tcaccgggtt tgacgatgat ataggcgata ttcatctcga
     8881 cgcggacgct ctggtcgtca ggattccagg tcacggtata accgagctgt tccgccactt
     8941 tacgcagagg aatcatgacc acgttatttt cctgtaatac ggctccatga gccgggtcca
     9001 tcaccttgcc ggcaacggtc agctggcact gctggatcgc cgtcagcccc ggaatcgacg
     9061 tatacggccc cataaccggc ccttcatcat aggcccaggc cggcccgcaa gccaaagccg
     9121 ccacgaccat cgctaagata atactcttct tcataaggga ttcccccttt atccaaaaca
     9181 caaaaatgag tcataaataa ggacatatat aatatattcg acaaatcccg ccgttttcct
     9241 gctaatattt aaaaacaacc tctcagaccg ccttcggcgg ccagctcccc tataaaggga
     9301 gccttgcaaa aaaagcgctg ccatagaaga cagcgctttt ttgcaaaact aaaaatcaaa
     9361 gaatcccgtc tggtcggcca tgggtacgac ggggaatccc gtcggtactg gctggatgat
     9421 catgaacacc agggctgcca ggacggcaat gcccagggtg aggtacgaga agcttccctt
     9481 ttctttatgt tcatacaagc cgaacaggcg gatgccgacg gccgagccga tagtagcgaa
     9541 cagggcgccc agcgagaaat actggtacat ggcatacgac gccagcaggc cgatgatcat
     9601 ggccccgttg atgacgatgg gatagcactt ggacaacacc gattcgggat tgcggcgcat
     9661 cgccgtaagc caatcggtcc cgtcgatacc gcgcaggccg gtgaggaacg tcgatgcgta
     9721 gagcatctta tcccaccagg ccgtcccctt gcggcggtct cccgtccgcc acatgacgaa
     9781 ggcgtaaatc acgatggtca cggcgccgac ga
//
LOCUS       DAWCYS010000039         9810 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_452_length_9810_cov_8.706817, whole genome shotgun sequence.
ACCESSION   DAWCYS010000039 DAWCYS010000000
VERSION     DAWCYS010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_9810_cov_8.706817"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3196..3205
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2337 a         2631 c         2489 g         2343 t
ORIGIN      
        1 gtaaaggatg tccttgcaca aaatgtaaac gatgttattc ttgacacacg tggcgacccc
       61 tacaaagtaa aaaggcttgc cgcacatgcg acaagccttt tttactttcc ctctgcgatc
      121 agccctgatg gaattctacg cccatagcgc ctttcggatg tttccagctc tttacgcctt
      181 tgccgccgga aatgacgcgg cacgtaccac attcgaggca gccttcgtga ctgaagacca
      241 gtttgccgtc atcatagtga tacaggttgg ccggacatgc catgactaag cgtttgattt
      301 ctgcttcgtc atgataatct tcgttgatca agatatggga ttcgtgttca tcggtcacga
      361 acttatcgaa acccagttta tcgtctacac tcatctttgc catcttacat cgcctcccaa
      421 gctttcttac ctaaatcgaa gagattagcc aagccaccgt tgtttgccaa gactgtcaga
      481 gcaatcatgg gcagagcgac cggtgcgccg ccgtctactt tgtagaccat gccggcaaat
      541 tcgtcggcca tcttcgggaa atcgttgaag atgatatgat cttccaggag gcccggcagg
      601 ttctgatact gtttcatgag cttcatgatg aagctttctt ctaacaaggt cttgtaaatc
      661 gagaggcttt cagccgagaa gtcgttgcgg ttcttcgctt tgatgaccgc ttcgccagcc
      721 agacggcccg attcgatagc caggtccatg ccgcggacgg tgtaaccgag gttgacgacc
      781 atagcagccg catcgccggc gacgaggacg ccgtcacgga cgagttccgg catcatcttg
      841 aggccgcctt cggtaacgag gtgagccgaa tattctttca gtttcccgcc tttgaggaag
      901 ggagcgattg ccggatgagc tttcatgcgt tcaaccatat ccggtacact gacatcgacg
      961 cggccgatat cgctgatggt cgtaacgatg cccagggaca ggctgtcttt gttggtgtaa
     1021 atgaagccgc cgccgaggtt gccgcccgtc gggtcgccag ccatcatcca ggctgcgccg
     1081 tcattgccgg tcaggccgaa acgttcgttg atgacgtctt caccgagttc gatgacttct
     1141 ttgacgccga cagcaacgtc tttctgttcc agttcctgtt tcatgccgat ttgctgtgcc
     1201 agcagggagt tgacgccatc ggccaggatg acgacatcag cgtcaaattc ttcgccgcct
     1261 gtcatgacgc cgacgacttt gccgtcttcg acgacgagtt catcgacgag gaagccttcc
     1321 gtcagcataa cgccggcttc ttcagcttta tcagccaacc atttatcgaa tttagaacgc
     1381 agtaccgagt aggacggata tttgaggttt tccagtttat ccgaaccata ctggaagctc
     1441 agtgcgccgc cttctgtcat gagcgacagg cgttcctgcg taatcttacg ttcaatcgga
     1501 gcttctgctg cgaaattcgg aatcagtttt tccaagctgt gtccatagag acggccgccg
     1561 ctggagttct tggaaccgca gaatttaccg cgctccacca tcataacatc caaaccagct
     1621 ctggccattg tcaaggctgc agcagaacca gctaaaccgc cgccaaccac gatggcatca
     1681 aatttttctt cactactcat gttcttaccc tccttaacca tacggtttgc tatcttaatt
     1741 tccgttcgta tctatcgtaa cgtaatgtaa gtccttttca taatccttca ttcgaccgca
     1801 ggcttcttcc ccttgtggag cacgcaggcc ccaaataagc ggcttttcat ctgcctgtca
     1861 atcaatccgg cttgtccaaa caagtccagg agctccttct tcctgagaaa atcctgcgtc
     1921 gactgccaca gccaggtgta ttcctgccga tggcggaagc cgccgcccag gaagggcatg
     1981 acataccgga agtacaccga ataaaagggc cggatgacca gagaatcggg gacaaaggag
     2041 tcgagacaat atacccagcc gccgggaacg acgacgcggg tcatttcctg caggacgcgc
     2101 aggtagtccg aggtgttgcg aagcccgaag gagatacagg ctgccgaaaa ggtattatcc
     2161 tcaaaaggca ggttcatggc atcaccttcc tgccaggtaa cgttagcaag cggcgcgcct
     2221 tttttgcggg ctacgtcgag catcgccggc gaaaaatcga ggccagtgac cttgagatcc
     2281 ggccggtgcg ctgccaggga aatagcgatg tccccagtgc cactgcacac atccaggaca
     2341 cggctgccct tggctgcctg ctcacagaca gcttcgatga ggcagtcttt ccaacggctt
     2401 tccatgccga gactgatgcg cccgttggcg cggtcataag tctgggaaat ctgctggaat
     2461 acatgatata ccttctcccg ttttgagagt tcagccaaaa gtaccacctc cgaagaggac
     2521 catcgcgagt gctaacagat aggcaccgtt gccaaagaga ttggcggcga tgataccttt
     2581 catctgtttc acccgcccct gcggttccag aggcgaccga agcagattga caaaggtatg
     2641 tcgggcaaac acgataaaga aagccgggct gatgaggccc caccaaccga tataccatac
     2701 gggcaggagg cagatgagtg ccatccagat ccatacggca gcccggtaca ggcggacggc
     2761 ctgagtcctt cccaagagtg ccggtaaggt ccggcgcttg gcctgcctgt ccttttcaat
     2821 atcactcgta ttattggtca tcatgatgag accgatactg atgatgaagg gcagagccgc
     2881 aggcaaaacg gaaaaatcga aggtactgct gacggcggcg acgattccca tggggatgag
     2941 gccgcccatg acaaagcccg aaacgaactc gccaatcggc agataggata tcggcaggat
     3001 accaccggaa taggtccata cggcgatggc tccgatgacg ccgatgatca gcggcgccgg
     3061 tccggctgtg accagtgccg gcaaggccag gagaccggct gcaactaagt agccaagtcc
     3121 caaagccaat gcctgtccag gctagatgtt ttcataaacc aggacggcgt cgctcacctc
     3181 gacgttgtcg tcggcnnnnn nnnnngtcgt cggcagtgtc cgtgcctttt acaaaatcaa
     3241 aataagcatt cagcgtattg acggcggact gcatgaagac gcaggcccaa aagagggtga
     3301 tggccagcgt ccccgtaagg gggtagccct tcaggaggca atacagttca ccgaatacgg
     3361 cgggatatac agaagccagc caggtgtgcg gtgcagccag gcgaacggcc agcttaggtg
     3421 ttaagcaatg ttgctgttcc attcacacgg cccccagctt acgtacgatc ttgccgagct
     3481 gcagggaaat atcacttgac ggcagggctt cgaggatgtc ctcagcccgt ttctggtagg
     3541 tgtggatctg gttccacgcg ccttccaggc cgcccagggc tgcgacgatt tcttccatgc
     3601 ggcagatatc cgcatgagtc agcgtccctt cggcgctcct ttccatatag gggtagagga
     3661 gccggcggcc gccgggctgt tcccgtgcca ggaggaccgg cagggtatag atcccttcgc
     3721 ggaaatcctg gtgggctttc ttgccaatca tcgaactatc gggtacgaag tcgaggaggt
     3781 cgtcgcgcat ctggaacata tagcccagac attcgccgaa ggactcgagc atccgcgata
     3841 cccgttcgtc acaaccgctt tccatggcac cgatgcggca gcaggcgcgg aacaaggcga
     3901 cggtcttgcc gtggatgttc tgcagatatt ggtcaatagt gacgtcctct ttatagcggc
     3961 acatggcctg gccgatttcg ccggcacaca tttcctctac aacctgggcg atgaccatac
     4021 ctgcccggtt caatccctcg cgcatcaagt agaaggtgat gcggctcatc aggaaatcgc
     4081 cggcatagac ggctgcgttc ttcccgtact tgctctgcac cgacggcttg ccgcgccgga
     4141 ccggtgcatc gtcgacaata tcgtcatgga tcagtgaggc cagatgggtc atttccacta
     4201 cggccgccag tttatacaac cgctcccgca catcgtcgcg gtctgttccg aacgagccgg
     4261 ccatcagtac caggcgggga cggatcatct tcccctgcga agaggctgca tcgtcgagga
     4321 cagcgccagt ggctgtacct tcaaagggat gctgcgtata tagttttata tactctttca
     4381 ctctgcggac ctggtcatcg ctgataccgg caaacaattt gggcataaac ttccgggcgc
     4441 tggtttctaa gagtgtctgt tttatcatca tgctctattt acctttccgg ccaacgttat
     4501 tgcctatttt ttttcgcgtt tttcaacgac gatctgtgct gtgtgtttaa cctgaatgtt
     4561 gttcaagccg cgagcatcga ggccgccacg gatctggagc aagcagcccg ggcagtcgac
     4621 agcgacgaca tctgcgccag aagcgacgat gttgttgatc ttttcttcga ggatcggagc
     4681 cgacatttca gggaatttaa cggagtaaga accaccgaag ccgcagcatt tatcgcaatc
     4741 gtgcatttcg atgagttcga cgcccttggt agctttcaag agttcacgct gttccttggt
     4801 gacgccgagg taacggttca agtggcagga gtcgtgatag gtgaccttca tcggtacgcc
     4861 atcgccggta tccggcaagc cgccgagcat ggaaacgagt ttgcagaagt cgaaggtctt
     4921 gctgcggagt gcttccgctt tcttgagcat ttccggatcg tccttgaaga agtcgacata
     4981 atcgcggagg gcatgcgtac aagtcgggca agccgatacg atgtaatcgt atttttcagc
     5041 ttccatagcg ttcaagttca tttcagcagc tttcttggcg ttttcaacat cgcccatata
     5101 ggtagccgga gcaccgcagc aagcctggcc aagcggcatt tctacgatat agccagccat
     5161 gttgagggct ttgacgacgt ccgttgcgat gtcgacatag acgaagtcga ggagacagcc
     5221 cgtgaagata gcgatcttgc ccttcggatt gggaacatcc tgtttgattg tcggcaggat
     5281 atcgcggaac ggctgcggag caacgctcgg cagactgcgg cctgctgtca gaccggacaa
     5341 gaacatcggc agatgacgga tcatcggctg acctttggta atcatgccct gagcaacgga
     5401 agcgatgcgc agcatagagt ggaagaggtg acggtcagca accgtatcga gacagaattt
     5461 atggaccgga ttgaggccat cttcctgagc gaatttcgta cgtaatttca ggatcatgcc
     5521 ggcaatatcg agtttgccgc cgcagactgt gttacacgta ccgcactgga gacagatggt
     5581 ttggatatct ttaccgcgtt cacggctgtt gaggaagctc gtgagcagcg tgccgatacc
     5641 gccggtataa atggagccgc catatacgtg gccgccgacg aggcggaagg ccggacatac
     5701 attcaggcag gcagcgcagc ggatgcagca gaagatatct ttgtaatctt cactggccat
     5761 gatcttacgg cgttccggcg tatcgaggat gatcatgtgg aagttcttgt cttccttcgt
     5821 atcgttttcc ggatccgtaa cgactttcgt agcgcccgtg tagaaggaaa ggtatgccgt
     5881 gatgttctgg gctgtaccgt tacgaggcag tactttgaag atgtagcgga tatcgctcat
     5941 cttagcgacg aatttttcaa taccaaagat atataagtgc gttttcggaa gggttgcgac
     6001 catacggccg ttgccttcat tcgtcatggt aatgactgtc cccgtttcag cgacagcgac
     6061 gttggcaccg gaaaccccca tttcagcgct gaggaatttc ggacgcatga cgcggcggga
     6121 cgtcttgact tctttcgtga tgaccggttc ttccggctgt ttcgtatacg attcaaagag
     6181 gtcggcaacg tcttctttag aataatgaat agccggcata accatgtggg acggcgtatc
     6241 accagcgatg gagttgatga attcgcccaa gtccgtttcc tgagccgtaa tgcctgcttt
     6301 ttcatagttc tggttgaagt gcatttcttc cgatgccatg gatttcgact taacgcaaag
     6361 tttgacgttg ttgtcttttg ccagtttcaa agcatagtcc atagcttctt ccggagaatg
     6421 agcgacgaat acgtgagccc cgttggcttc cgcattcttg atgaattcgg ccatgacttc
     6481 gtccagatgg tcacgagcat aggattttac ttcatggact ttctcacgaa gcgcttcaaa
     6541 gtcatatcct ttgtaaactc ttgcacggtt gccaggataa gctgtcgtaa agttcttcaa
     6601 cgccttctgc agcggcgcat tatccatggc gcgctcgatt tcttgcttca gtaattcgtt
     6661 agacatgtgc tgttccctcc cttacgcatc ttctacgacg atgaccgaat actgacgcgg
     6721 gccatgaacc cctacagtac cgacacattc gatatcagct gtccggctcg ggccggtgat
     6781 gataccggcg aatttcggga acggtttcgt ttcggccatg atgtcgatca tgtcatcgaa
     6841 agtatcgacg atcttagaaa gtttgacgat gccgacatag acgtccgata ccgtttcaac
     6901 gatacgacag tcgatgtcgt ccttcatctg gacaatggag cccaagttgg cgatgccgta
     6961 atcggcttcg gtaacgccgc ctttgtcgtc cggggcattc ttgcggaagt gatccgtatc
     7021 cactttgatg cctgcggctt tcaatgcgtc tacagcgccg atttctttca ggagcggcgt
     7081 ttcaacgagg gcaacttcat gtgtgtcctt gtcctggaaa attttggtga gagttgcagc
     7141 aacgtctgct tttttggcga tgtaactttc gccgttgacg ctggtcaacc ctttctggaa
     7201 tgtttcatac aattctgtct tttccatttt cacacctctt tacttataaa tcacaactac
     7261 aacgaatact atgaagtaaa gtatttaaca taagtatacc atttcatgcg caaagtgcaa
     7321 taagtataga aaaatgggta atacaaaatg agaaaccttg aatggtcatc tatgcgtttt
     7381 tgttattagc tcattcatta ctaatatggt gttcttttaa cactccatgc tttttatcaa
     7441 taagcctttc agaaaaatgg cttatttacg ctatatactt cgcttaacca cgctaaagga
     7501 tatgtttttg taatgatatt tttagatttt ttaaattact ttatctcata ttaagctcat
     7561 tttgctttac agtatacgat tgattgtaga gaatacaatt catatctact aaacatggat
     7621 attgtatgca gacaaattta atcaaaaata atgagtcttt atgaaaacat cgttcatcgc
     7681 aggccgttcg tcgtaggtcg aaaaaaacaa aagccctggt tacgacgaac gatgaacgaa
     7741 aaacggcaaa tcccttatat ggatatataa agaacgtgat tctatcggcc tttttgaata
     7801 agacaccaat tttgtcatct ttttacaatt tttctattga caaacgtcct gtgaaggggt
     7861 atcatgtgtt caacacgaaa acaaaaatgc aatgaccaaa acaagtacac acaggataac
     7921 ggcacagaga gtaagggcca ccggctgaaa gcccctatcg aagttgaatg tgatggaatg
     7981 cctttgggag caggatgatt gaacgtgagt aggtcatccc ggttgccgcc gttacgggta
     8041 ttgagtggca gtcgaaagac tgcaatcaga gtggaaccac ggaccaccgt ctctgtatga
     8101 cggcaggtct ttttttattt acttttttac gacacaggag ggcgctacgc catgttcaaa
     8161 tcacttcgca acgacattcg cgtcatcaag gaccgcgatc cagccgccaa gaacacgctg
     8221 gaagtcttgc tctgctattc cggccttcat gccatctggg cccatcgcct ggcccacttc
     8281 ttctataagc atcagtggtt cgtcacggca cgcctcattt ccacgatttc ccgcttcttc
     8341 acgggcatcg agatccatcc cggcgcccaa atcggcgaag gcctgttcat cgaccatggc
     8401 atgggcatcg tcatcggaga aacgacggtc atcggcaaca acgtgtccct ctatcagggt
     8461 gtcaccctcg gcgggacggg taaggaaaag ggcaaacgcc atccgaccat cggcgactac
     8521 gtcgtcgtcg cctgcggcgc caaggtcctc gggtccttca ccgtcggcga aagctcaaaa
     8581 atcggcgccg gttccgtcgt cctcaaagaa gtgccgccgt actcgacagt cgtcggcatc
     8641 ccgggccgcg tcgtcgtcca gaaaggcaag cgcctcaagg ccagccacag cgaacgcgac
     8701 gtcgacctca accacaaccg cctgcccgac cccatcgaag atcagatcat cgccctgcag
     8761 catcaggtag ccctcttgca gcaccgcgtc gaaatgatgg accaccagca agatgaacgc
     8821 tgcgttggca aaatgcggcc attgaaataa taaacttcgt tgtcaccctg tatggaaatg
     8881 aaaaatccct ctacaggtgt acataaaatc tatacccatt tggtatacct tactatactt
     8941 caacatattt ttgcattcat cgggggatga atcataggaa aaggagagat tctcatgagc
     9001 aaaatctaca catccatgtt acagttgatc ggcaacacac cattattgaa accggaacgt
     9061 tacaacaacg ccgctggcgt agctgctaac cttttggtca aactcgacgc tttcaacccg
     9121 gccggctccg ccaaagaccg catcgccaaa gccatgatcg aagacgctga aaagaaaggc
     9181 atcctcaaaa aaggctcggt catcatcgaa ccgaccagtg gcaatacggg tatcggcctg
     9241 gctgccgtag ctgctgccaa aggctaccgc gccatcttca ccatgccgga aaccatgtct
     9301 atcgaacgcc gcaacctcat ttccgcttat ggcgctgaaa tcgtcctcac accgggcaag
     9361 accggcatga aaggcgctgt cgacaaagcc aacgaattgg ccaaagaaat ccccgattcc
     9421 ttcatccccg gccagttcac caacccggtc aatccggaaa tccatcgcct gacgacgggc
     9481 ccggaaatct atgaagatac agacggcgct gtcgacatct tcttagctgg tgtcggcaca
     9541 ggcggcacgc tctccggcgt cggccagtac ctcaaagaaa agaaaccgtc cgtccagatc
     9601 atcgctatcg aaccgaaaga ttcgccgctc ctctccgaag gccatgcagg cccgcacaaa
     9661 ctccagggca tcggcgcaaa cttcgttccg gaaacattgg ataccaagat ctacgacgaa
     9721 gtctacacgg ttacgacaga acaggctgat gaaacggctc agaaattcac cgaatccgaa
     9781 ggcctcctca tcggtaacac cggcggtgca
//
LOCUS       DAWCYS010000040         9373 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_477_length_9373_cov_5.939472, whole genome shotgun sequence.
ACCESSION   DAWCYS010000040 DAWCYS010000000
VERSION     DAWCYS010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_9373_cov_5.939472"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2018 a         2676 c         2648 g         2031 t
ORIGIN      
        1 tccccagcgt tccccggtct gggcgcctca ggctcacttc ataaaagccg atgatcgggc
       61 gctgggacac gttgaccgtc aggccgtctt cttcaatgat ggaaggcccg acgaagtgga
      121 aaggtttgat gtcctggtga tagtcgtagt ccttgaagcc gacgatctgc gaatgggggt
      181 ggtggatggt gccgccggcc atgtagccgt ggttgcggta gaagacgacg gaccggaatt
      241 ttcccatgtc catgacttgc tgccattttt ccaggccgaa ttcgatgagc ttcaaggcgt
      301 attccggtgt atacgatgaa aattcgccat cgcagtcatc gctttcgatg atcagcgtct
      361 gccacgaatc cttgaggacg gggtaggcgt tttccagcca gatcataggg ccccgttttt
      421 ccatgacgtt cttcaagtgg gctacgtcgc agaagggaca atacttttca tcgcgatgga
      481 ccgtgtcggg cttggtcttg cccaggtcca gattatattc caaaatgtga gataagggat
      541 tttcattcat gacgaccgct ccttttgata cctactatac attgtagcat agagatagcc
      601 attttcaaac catgtggtat aatacagata tgctattttt aaggaggaat ttatcatgag
      661 tcttcatatt gctgctcgtg acggtgaaat cgccgaccgc atcttgcttc ccggcgatcc
      721 cctgcgggct aaatatattg ctgaaacttt tttggacgat gccgtctgct acacggatat
      781 ccgcaacatc ctcggctata cggggaccta taaaggcgtc cgcctgtccg tccagggcac
      841 gggcatgggc atcccgtcca tttccattta tgcgacggaa ctcatgcgcg attacggcgt
      901 gaagaagctc atccgcgtcg gcacctgcgg ggccatgcgc aaagatatca agctccgcga
      961 cgtcatcatc gcccaggggg ccacgacgga ctcgtcgatc atccgcaaca tcttcggcgg
     1021 ctccatcaac tacgcgccac tggctgattt cacgctcctg cgccaggcct acgaacagtc
     1081 ggtcaaacag ggcatcccgg cgcgggtcgg caacatcgtc tctgtcgacc gcttctacga
     1141 tgacgaaatc gacaatgata aactgaccca gtacggcatc atggccgtcg aaatggaaac
     1201 ggccggcctc tatacgctgg ctgccaaata cggcgctcag gccctgggat tgttcacggt
     1261 cagcgaccac ttgctgacgg gcgaagcctg cacgccggaa gaacggcaga cgtccttcga
     1321 cgatatgatc cgcattgcct tggaaacggc tatccaggag taaccgccta tgagagctaa
     1381 agaactacgg gtcattgaca gccacttcca tttttgtgat tacgtcggct tcaaccagat
     1441 tgccctggct gcgggctata ctaatacgga agaatgcctg cacaaggcct ttcaggacaa
     1501 caacatcgtc cacggcgtcg tcatgggcaa caagaccctg gacccggcag gccatcacta
     1561 ccccgattac atgagctact gcatcggcct ggacagcaac gtcttttacg acgatatgga
     1621 cctcatgacg gcccaggtcg aagagaacct caagcgcaag cagtgctgcg gcatcaagct
     1681 ctatccgggc tataaccacg tctacgtcta cgatgagctc tatgacccca tttacgagct
     1741 ggcccggaag tataacaagc ccgtcgccat ccacacgggc ctgacggcta cggccaacgc
     1801 cctcttgaag tacagtcatc ccatgaccct cgatgaagcc gcagtctaat atccctatgg
     1861 ccagttcgtc atgtgccaca tcggcaatcc cttcctgcag gatgccattg ccgtcctgga
     1921 aaagaaccac aacgtcgccg tcgacttgtc aggcctcctg gaagggaaga tacccgacat
     1981 ggtcaccttc ctgcgggata agcggggcta tatttccatg ctccgggact ggctgaacta
     2041 cctcggctct tatgaccggg tcatgttcgg gaccgactgg cccctggcta attttgccga
     2101 ttatattacc tttgtcaaag atttcatccc tgaaacgtat tgggatgacg tcttctttaa
     2161 caatgccaac cgcatctacc agctgggatt atagccggcc ttgcggcaag ggaggaacgc
     2221 cttttgtata ctgtaaccaa gcggatggaa atatccgctg cccattacct ggtcctcgat
     2281 tatgagagca agtgcgagca tctccacggt cataactgga tcatctctgt gacctgccag
     2341 gccgaaacac tggaccgaaa cggcatggtc gtcgatttca agaaaatcaa ggaaatggtc
     2401 cacgccaaga tggaccatca gaatctgaac gatgtcttcg acttcaatcc gacggcggaa
     2461 aatatcgcca agtggatctg cgacaccgtt ccccattgtg tcaaagtgac ggtccaggaa
     2521 agcgaaggga acgtggccac ttatgaatta tagcctctgt gtcaacgaga ttttcgacag
     2581 tatcgacggc gaaggcaaga aagccggtca gctggcgacg ttcatccgcc tctgcggctg
     2641 caacctgcgc tgttcttact gcgatacggc ctatgccttt aacgaaggcc ggcacatgga
     2701 cattgccgat atcatcgccc aagtgtccta tcccaacgtc accctgaccg gcggcgaacc
     2761 gctgtgccag gacatccacg ccctcctgga agggctcaaa gggcacagcg tcaatatcga
     2821 gaccaatggc agcatggaca tcgagccgta tttcaaatac gaccacgtct ggttcactgt
     2881 cgattataag agcctgtctt cggacatggc caatcgcatg ctgccggaga acttcgagaa
     2941 gctccggcct caggacgtcc tgaagttcgt cgtcggcgac gaagccgacc tgcgccaggc
     3001 tctgggtgtc tatcagctgt accggcctaa atgtgccgtc tacgtcggcg ccgtcttcgg
     3061 caaaatcgag ccgcggcgca tcgtcgagtt catgaaggaa caccgtctga cggactggca
     3121 catgcagctt caactccata agatcatctg gaaccccgac gaccggggcg tctagctggg
     3181 acgacgaaac gccgttatcc gctgatttgc gggtaacggc gttttttgaa gatggcctgg
     3241 tcgtcgtcga gcatgtgctg gaagcagaag agcgtcgcgt ccgtatcggt caggctgtcg
     3301 tgccagttgc ggccgtgata gccgtagtgg gcggcgcagt tcatgagctt ttcataagtg
     3361 atggtcttcg tttcctggta atggatgagg gaaaaggctt cggccaggtc gaggtagatg
     3421 tccttgtccg gcaggacgac gcccgacgcg tggagcttgg caatgtcgtt gaaggtactg
     3481 tagccgacga tgaggtctgc atccatgaaa aggctgctga ccgtgtcggc ccagaggatg
     3541 aacgggtctt cacaggcgac ctgttccggc gtgatgtgat tgacttccat cgcttcatcc
     3601 cagcgttccg tgtagagggg cttgaaatag cggttcatga ggacgtcgcc gcagccgttc
     3661 atgatggaca gctggatgat ttcggcgtcg gccgtgaggc ccgtcgtctc caggtccagc
     3721 gtcaggatgc gcgccgggtc gaggcggcgg atggcctgtt tgatctgcgg tgaccggcgg
     3781 atgctctggc gccgggcttt ttctttcttc gacagtaact ttttcatgga aaaacctcct
     3841 ttcttctctg caatggcttt attataggaa gaaaggaggt ccttgtcaat cgttatttct
     3901 tggtgctggc accggtcgca gcctgcggcg gagccggccg gtcgacggtg gcaaagccgt
     3961 agacggccgt acaggccagg agcagcatga gcaggaggga gatgattttt ttgctgtcat
     4021 aggagaagtg tcctttcgtg attacttatt cgtataagcc gctgggcttt tccgagaggt
     4081 tgatgaagat attcttggtc tgcatgtaat ggcgcatggt cgtcttgtcc gtttcgcggc
     4141 cgatgccgga gagcttgtag ccgccgaaag gtgcgccggc cggcagggca ttgtaggtgt
     4201 tgacccacat gcggcccgtt tccacgcctt tggctacgcg cagggcccgg ttgatgtcgc
     4261 gcgtccagac ggcgccgcct aagccgtatt cgctggcgtt ggccagggcg atgacttcgt
     4321 cttccgtctc aaagggcagg atgcagacga cagggccgaa gatttcttcc tgggcaatgc
     4381 gcatatcgtt ggtgacgtcg gcgaagatcg tcggtttgat gaaattgccc ttgtcgaggc
     4441 cgttggcggt tgccttttcg ccgccgcaga ccaggcgggc gccttcctgc ttgccgattt
     4501 cgatgtagct gaggatcttt tcgacctgac ggccgtcaat ctgggcgccc atctgcgtgt
     4561 cttccagcca gggcaggccg actttgacgc ggttgaatcg ttcggccagt tcggcgacga
     4621 acttgtcgta gatccctttc tggacgaaga tgcgggagcc ggcgcagcag acctgaccct
     4681 ggttgaagag gatgcccagc tgggcgccgt ccatggccat gtcccaatcg cagtcgtcga
     4741 agaagatatt cgccgatttg ccgcccagtt ccagtgtcgc cgggatgagt ttttcctggg
     4801 ctgctttgta gacgccgcgg ccgatttccg tagagcccgt gaaggccagt ttctggatat
     4861 ccggatggtc gaggatgtac tgacccgacc gggagccctt gccggtgatg acgttgacga
     4921 cgccgtcagg caggacgcct tccaggaggt gcatcagttc caggacgctg agggacgtcg
     4981 tgctcgatgg cttgaggatg atcgtacagc cggcggccag ggccggtgcc agtttccagg
     5041 cggccatgag gaaggggaag ttccacggga tgatctggcc gacgacgcca atcggttcgt
     5101 ggatgaccag gctcagcgtg tggtcgtcca gggtcgacgc ccggccgtct tcgctgcgga
     5161 cgacgccggc gaagtagcgg aaatggtcgg ctgctgccgg gatgtcgacg gccttcgttt
     5221 cccggatggg cttgccgttg tccaaggttt cgatgagggc cagcgtgtcg gcattcttat
     5281 cgatgatgtc ggcgattttc aagaggatgg cctgtcgttt tcggggcgat acgttcttcc
     5341 aggccgggaa ggcagcctgg gcggcgcgga cagcggcgtt gacgtcgtcc tgcgtcgctt
     5401 cggcacagac cgacaggact tcaccgttgg cagggttgat ggctttaaag gtagaaccgt
     5461 cgctggacgg gacccagcgg ccattgatat ataaatcgta agaggggcgg atgacatttt
     5521 tcatcaggac acttcctttc tacgtaatgg acataagaga aaatgattct ttgttaatgt
     5581 aaagtcatta taccataagt atactaaaac ggtcaacaat gggaattttt tcacggtctt
     5641 tgccctgtat aaatgattgc aaatattgac aaagcaggct aaaatagata gggtacacaa
     5701 taagtatagt ttcacaggag aagttacatg tataaatacg ttttattcga tctcgacggg
     5761 accctgaccg attccaagga aggcatcatc aagtccgttg cctatgccct ggaacagatg
     5821 ggcgaatcga cagaaggccg tctggatcag catactctcg tcgggacgcc ggtgttgccg
     5881 agctatgaac acggctaggg cttgtcgccg gaactggccc gtcagactta tgcctatttc
     5941 caggaacgct acagcacgat cggcaagttc gagaaccggg ctttcgacgg catcgttccc
     6001 atgctcaagg ccatgaagga agcgggcatc cgctcgttcg tcgctacatc gaagccccag
     6061 gtccatgccg aggcaatctg ccagaaattc ggcatcgatc cctatgtgga agccattgcc
     6121 ggaccggccg tcggcgggac tgataccaag gctgacgtca tgcggcgcat cctggctggc
     6181 cggggcccgg tggaaaaggg cgacgccgtc atggtcggcg accgccggtt cgacatcatc
     6241 ggcgcccacg aattgggcct gcccgtcatt tatgtcacct atggctatgg cgatgaagcg
     6301 gaacggaagg ccttccagcc cgattacacg gcagcgaccg taggggactt gaaacagtta
     6361 cttttgcaag catgatgcta gctataatac attatataat aagagatgaa ggagcatttt
     6421 tatgaagtta caagaaaaac agatgatcca tggctttgcc gtcgaccggt cccgttacgt
     6481 accggaactg cacagccagg cctatgagct gcaccacgtc cagtccggtg cccgcctgtt
     6541 atatatccag aatgacgacg acaataaggt cttttccatt tccttccgga cgacgccgtc
     6601 cgacagcact ggcgtacccc atatctgcga acattcgacg ctctgcggct cgcggaagtt
     6661 ccccctcaag gaaccctttg tcgaactggt gaagggctcg ctcaacacct tcctcaacgc
     6721 catgaccttt ccggacaaga ccatgtatcc cgtagccagc cgcaacgctg ctgatttcaa
     6781 gaacctcatg gacgtctatc tcgacgccgt cttcttcccg aacatgatta aggacaaaca
     6841 ggtcctcatg caggaaggct ggcattatca tctcgactcg gctgacgacg agctgactta
     6901 tcgcggcgtc gtctataacg aaatgaaagg cgtcttctct tcaccggact cgcagatgga
     6961 acggcacgtc atggatgccc tcttcccgga tacgacgtat ggcgtcgaat cgggcggcaa
     7021 ccccgatgat atcccgaact tgacgcagga aggctttgcc gctttccacg ccaaatatta
     7081 tcacccgtcg aacagctata tcttcctcta cggcgatatg gatatcgacc agaccctggc
     7141 cttcatcgac ggcgaatacc tgagccagtt ccaggcccag accatcgatt cggccatcgg
     7201 ccgccaggcc tgcccgggca gccaggtcca gaccttcccc tacggcattg ccacgggaga
     7261 aaagacggac cacaagacgc tgcacagcct gacctatgtc atcgacgacg ccctggaccc
     7321 gacggtagcc atggccttca aagtcctgac ctacgtcctc ctgcagtcgc cggcagcgcc
     7381 gctgaagaaa gccctggtcg atgccggtct gggcaaggat atttccggtg acttccagga
     7441 cggtctcctg cagcccatgt ggggcatttc cgtcaatggc tcggacccgg ataaacagga
     7501 caagatcctg cccatcgtcc gcgacgtcct cagcaacatg gtccgcgacg gcctggacaa
     7561 gaccctgctc acaggcgctt tgaaccggac ggaattcatc ctgcgcgaag ccgatttcgt
     7621 cggccgcccg aagggcctca tctacggcat ccgctgcatg gatacgtggc tctacgatat
     7681 ggatcccctg gctgccctgt cttatgaagg ctccctggat gtgttgcgcc gcggcatcga
     7741 caacggctac tttgaaggcc tcatccagaa atacatcctc gacaatccct attacgccct
     7801 gatctcactg gtaccggaac cgggcctgac cgaacggcac gacaaggccc tggccgacaa
     7861 gctggctgtt tacaaggctt cgctctcgaa agaagaaatc gacgccatcg tagccgactc
     7921 ccaggccctg cagaagcggc aggccacgcc ggactcgccg gaagccctgg aaacgattcc
     7981 gaccctgacc cgggacgacc tggaaaagaa ggccgacacg attgccatgg aacagcagga
     8041 tgtaggcggc gtcccggtct gctatgtcgc ggatgaaacg aacggcatta cttatgtcaa
     8101 tgcctacttc gacctccacg gcctgacacg ggaagaaatt tcctatgtct acctgctgag
     8161 cgacctcatc ggcgatatgg atacgacaga ccatacgtat ggggacatcg cgtcgctcat
     8221 cgacttgtac acgggcggca tcgactacag cgtgtcggcc ttttcgaacc ggacggacaa
     8281 caaggactac atgccggtct tccgcttcaa ggccaaaggc ctgacgcaga acctggacaa
     8341 actcgtcgat ttgttgaagg aaatctccct gcatacggtc ttcacgaata aggaccgcct
     8401 ggtcgaactc gtggaagaaa cgaaggccgg ctgggatatg gatgccttcc gccggggcca
     8461 tacgatcgtc atgcaccgcg tcttgtccta cgtttcgcca gtcgaagcct tctgcgacgc
     8521 cggggaattg tcgtactacg aattcattac cgatgtcgcc agccgcatcc acacggacgc
     8581 cgacgccctc ggcgccaaac tcgctgcggt catgaagaag atcttcaccc gttcggccct
     8641 gaccctggaa gtgacggcca gccgggacga ctggcagacg gcgaaaaaca ctgtgtcgtc
     8701 gtggatcacg gccctgccgc agggcgaaaa gctccagggc ttgtgcgatt tcggcctgtc
     8761 ccggaagaat gaaggcatca tgaccagcgg cacggtccag tacgtcgcca aaggcggcaa
     8821 cttccgcagc cacggctacg actacgacgg ctcgctcatg gtcctcgata cgatcctcca
     8881 gtacggctac ttgtggacga agatccgcgt ccagggcggg gcgtacggcg ccttcacccg
     8941 tttctatgac aacggcgaca tggtcttctg ctcgtaccgc gaccccaatc tccgctccag
     9001 cgtcgaagcc tacgatgccc tggcagatta tctggaatcc ttcgacgtgt ctgaccggga
     9061 aatgacgaaa tacgtcatcg ggaccttgag tcgcatcgac atccccttga cgccgtccct
     9121 gcggggggcc aaggccatga cccgctattt cacggggacc actgatgcca ttgctcagca
     9181 gcgccgggac cagctgctgg cgacgacggc cgccgacatc cgggctctgg caccgcgcat
     9241 ccgcgccgtc atggccgatg acaatgtctg cgtcatgggc agtgaagcca agatacgcga
     9301 agcgaaggac ttgttcacct gccacatttc gcttccggat taggatgtga gtgtcctgcg
     9361 cggacgtttt gcg
//
LOCUS       DAWCYS010000041         9294 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_482_length_9294_cov_8.663708, whole genome shotgun sequence.
ACCESSION   DAWCYS010000041 DAWCYS010000000
VERSION     DAWCYS010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_482_length_9294_cov_8.663708"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2173 a         2547 c         2684 g         1890 t
ORIGIN      
        1 gtaattgata tcgctcatca tcgggctttc gacggtgagc atgatgactt cttcgatacc
       61 cgtatcggcg atgttcttac attcgcggtg gagttcttcg tccgtcaggc gggcgcgctt
      121 gatcttgttg tgcgagttga agccgcagta gatgcagtag ttatcgcagt agttggcgaa
      181 atagatcggc gtcaggatgg tgatgttgtt gccgaaatgg cgccgcgtga ccagatgggc
      241 tttctgggcc atttcttcca ggaacggtgc agcggccgtc gataagaggg ccatgaagtc
      301 ccgcgggtcc agatgttctt tcgacagggc caggcggacg tctttatcag tgaagtcgtc
      361 attgttgaag gaattgcgga tggcgacgac ctggtcgagc atatccgagt cgatgacttc
      421 catgcccggt aaatatttca tgtggtcgat gagtttgctc tgatcgatag ttttagtttc
      481 ttccgtcatg ttcgattcct cctcagtctc ctaagaatcc cgtcagcgga tccgaagccg
      541 aagccgtttc gcggacgcgg cccaagccgg caaggtaagc attgcggccg gcacggatgg
      601 cttcaccgaa ggctttggcc atgagaggca ggtcgccagc cgtagcgacg gcggtgttgg
      661 ccatgatagc agcggcgccc atttccatgg cttcgcaggc ctgggacgga cggccgatac
      721 cggcgtcgac aatgaccggc aggtcgactt cgtcgatgag gatctggatg aaatctttcg
      781 tcgacaagcc tttgttggtg ccgataggcg aagccagagg catgatggca gcggcgccgg
      841 cgtcagccag ggcacgggcg gcgtagaggt ccggatacat gtacggcagg acgacgaagc
      901 cttctttagc caaaatttcc gtagctttga tggtttcgtt gttgtccggc aggagatatt
      961 tggagtcgcg catgacttca atcttgatga aatcgccgca gccgacttca cgggacaagt
     1021 gggcgatgcg gacggcttcg tcggcattgc gggcactgga cgtattgggg agcagggtga
     1081 catctttcgg gatatagtcg aggatattgt ctttctggcc ggcttcgacg cggcgcaggg
     1141 ccatggttac catctgggca ccgccttgtt cgacagcggc cttgatgagg tcgacagaga
     1201 atttaccgga acctaagatg aaacgcgatg tgaattcgtg gccgccaagg accagggtat
     1261 cttttttttc cattgggaaa cactcctttt ataatgatag atgagttcat gactgctttg
     1321 ccgcagggaa tcagcccccg ccgacgaagt gtacgatttc catggaatcg ccgtctttca
     1381 aagtcacttt atcgtaatcc ttgcgcgata cgatatcacc gttcagctcg atggcgatgc
     1441 ggtctgtacg atattcctgg tcggtcaggt aagcgagcag gttctggccg gccgcttcga
     1501 tgtctttgcc gttgatccgt accatggagg tcctcctttc tctcaaatga aaaaagggtc
     1561 gcacgaagaa ttacacccaa ggtggtgtac cccttcgtgc gacccttgtg attcggtctc
     1621 actctgccta tgattatagc ctattgtcac aaaaagtcaa ccgttgataa tgaactgtct
     1681 tgagagaaat gaccggggaa gacgacaaag gccgaaaaac tatagtataa taaagttatg
     1741 attttacgag aggtgtgatc actgccatgg aagaacggat ggactggatg aatattgccc
     1801 gttattttta tttgaccgac gagcggctgc agcagatttc ccgcgatttt gcagacgaca
     1861 tggaacgggc cctgcaggga aagccgggag cgaccgtatc agccctgaag tcgtatgtgt
     1921 ccctgcctgg cggtgaggag cgcggtgtct atctggtcct ggatttcggc ggtacgaacg
     1981 caagggctgc gcggatccgc ctgttgggcc ggcactgtta cctcatcgaa aagaaggtct
     2041 gccagccgct ccggctgcct ggccagtacg actatctctc ttcggcgacg acggctgggg
     2101 agcttttcga tttcctggcc cgcctcgtcg gtcaggttgc ccatgggcag caggcctata
     2161 ccctgggcca taccttttcc tttgctatag agcggcagga cctgcgcgat ggccggctcc
     2221 tgtcgtggtc gaaagaaatt gccgttcccg gtgtggaagg cgaatgggtc aacgccatgc
     2281 tcaaacaggc cctggcccgt cagggcttta gggctgtcga accggtggcc ctggtcaatg
     2341 acacgacggc cctgctgttg tcggcagcgt atacgctgga acgggtccgc ctgggcctcg
     2401 tctgcgggac gggcttcaac gcctgctact atgagccggc ctgggatatg atcgtcaatc
     2461 tggaagccgg cgactatggc ggcctcatac gcaatcgctg ggacaaggca gtcgacgccc
     2521 tgtcggccca gcccggtcag catcggatgg aaaagatgac aggcggtgcc tatgcagcgg
     2581 aaatcttccg gcagaccttg ctgtcttatt tcaagactcg ccagctcccg gtgttttcca
     2641 cagccgctat gaatgacatc atcagctgcg acaatgattt gcagtgccgg ctcaagatgg
     2701 gccagctgtg gaagcgcatc gtgcggcccg atgagatgcg gcctgtccgc aatatcggcg
     2761 ccgccatttt cgtccgcgct gcccagctgg ccggcgccgt gagctgcggc atcctgcgcc
     2821 atctttatgg cggcgaagcc gttccggccc agtccgtggc cgtcgacggc agcctcttgg
     2881 aacacgtccg cggtgccttg ttcatgatgg aagatgccat gcaggcctgc cagaatgaag
     2941 gcgtgagccg ggacaagcag attcccgtcg acccggtact catccaggac ggccccttag
     3001 tcggcgccgc cgtcgcagcg gccatggcag gcgaaggccg gtgacagcag gccggcccgc
     3061 cacgaaccac gagccacgaa ccatgaacca ctagccacgg ccacgttttt tcggtacaat
     3121 ataatattgc agagatattt ccaccatatc tgtagttcag ggaaaggaga cggcgccagg
     3181 tcacgatgct gcaactcgat aaatctacca gcaaacccta ttacgtacaa atctatgaat
     3241 attatcgccg ggaaatcgaa gagcaccgga tgatagccgg catgcgcctc gactcggtcc
     3301 gtgaactggc ccaggctgcg ggcatcagca agatgaccgt cgaaaaggcc tactaccagc
     3361 tggccagtga aggctacatc ctgcgccgcc ataaggcccg ctatgaagtg gcctccctgg
     3421 gcggtctgga agcgcggacc gaagaggctg ctgcgcccgt attgcgtgaa gccagtcgtc
     3481 gccctgtcta tgcctatgac tttgccagtg gcgacatggc tttggaacgc tttcccctcg
     3541 acatatggcg gaaatatatg aatcggatct tatcggagcc agaccggctg ctggcggcca
     3601 atgatgacca gggcgtccct gatttgcgcc gggccttgag ccgttacgtc tatgaaacgc
     3661 gcggcgtcca tgcagagccg tcgcagatta tcatcggtgc cgggacgatt tctcttctcg
     3721 gcgtcctgac caatctgctt cacgataaat atgcctgcat cggcgtcgaa gacccgggct
     3781 tccgcctggg ccgggagatt ttccgcaaca gcggctattc catcgtgccc attcccatca
     3841 gcggcggact gctccacgtc gatgccctgg aagccagcgg cgtccgcctg gtctacgtca
     3901 gcccgtccca tcagttcccg acggggacgg tcatgcccgc cggcatccgc catcgtctgc
     3961 tgcattgggc tgaaaagacg gatggactga tcatcgaaga cgattacgac agcgagctgc
     4021 gctattatgg ccgccccgtc ccggctttgc agggcctgga ccgccacggc cgtgtcgtct
     4081 acatgggggc cttgtccaag gtattgccct ttttcatccg cctcagctat atggtactgc
     4141 cgcctcagct catgcccctt tacgaacagc ggcgcagcct gttccgccag ggcgcgtcgg
     4201 ttccggagca gtgcgttctg gcggaatata tcgagagcgg cgaactggcc cgtcaggtac
     4261 ggcgcctgcg caaggaatac caggaaaaag gggagctctt aggccggctc ttagtcgaag
     4321 cctttggtca ggatatcgtc gtagggcggc tggtttccgg cgtatactgc catatccgcc
     4381 tgcacagccg cttatcggaa gaagaactgc gccgccgggc ggaactgcag ggctgccgtg
     4441 tcttgtccat gcagtctttt tatgaaacac cgtcgcagga aacggataaa gaatttctcc
     4501 tgtcattttc aaaaattccc acctgcaggc tccgcgatgc cgtagcggcg ttgcatggag
     4561 cgtggtcaga gaaagaagga tactaatggc gaaaagtttg cgttttattc aatgcgggga
     4621 tctccactta gggagccctt tccataacct ctccgctatt gatgaacggt ggcagcgcat
     4681 cgtcgggaaa gcgccggtcc gggcgttcca gaaaatcgtc cagatggcca ttgataaacg
     4741 ggtccacgct gtcctcatca ccggcgatgt ctacaccagt gccgatcaca acttgacggc
     4801 acagctcgat tacgtgcgcc ttttacataa attagcacag aataacatcc aggtctttgc
     4861 cgtcctgggg aatcacgacc cgctcgaagc gtggaaggcg aagattccct ttccgccgaa
     4921 tgtccacgtg tttccgaccg atgctgtcga acgggtcccc ctggtcgtag atggcgaaga
     4981 agtagcggcc atttacgggc agagttatgc caagagcgaa cagcgggaca atctggcctg
     5041 gaaattcaac cgcgctgctg gcgaccgatt ttccatcggc atgctccata cccaggtagg
     5101 cagccgggat tcgacatatg cgccgtgcac cctggaagat ttgaaagaat gtggcatgga
     5161 ttactgggcc ctcgggcata tccacaagca tgagatactc tgcgaaaagc cctatgtcgt
     5221 ctatgccggc aacccgcagg gcctcgattg tacggaaacg gggccgcggg gctgttatta
     5281 cgtcgaagtc ggaccgtatg ggacgacgga cctggaattc atcgacacga gcatcgtccg
     5341 ctgggaaagc atcgacctcg ccatcgacgg tatcgaatcg gtgtcggaac tgcgcgagtc
     5401 cgtccgcttc gccaaggaaa aagtgcgccg tgatatcggc aagccgacgt tcctgacggt
     5461 caatttcacc ggttccggca gtatgtacca cgtcctcaac aatccggagg cgacgcagta
     5521 ctggctggaa agctggcgtg aagaagaaca ggggaaatac gccttcgtca tggtcgaacg
     5581 cctgcgtaac ctggcccgtc ccaagatgaa tatggacgaa cgaagtaaac tgcccgactc
     5641 cgtcggcgac tacttgaatg tcttcgacaa gctggaaaag ctggctcctg aagaaagggc
     5701 caaggcgctg cgggatattc tcatgagccg cccggaattt gaacgcctcg gggcctatgg
     5761 aagggccctg acggaccagc gcctgctgga aacgttcgag aaagccaagt ggctgggcgt
     5821 acagaaattg ctggaacata gaagaggtta agcagtatga agattacaga gatgcatttg
     5881 gatgatttcg ggatttacca taatgtgagc tggaatccgc cggaacacgg cctgatcgtc
     5941 atgcatggcc gcaacgaaag cggcaagacg acgctcatga aatatgtccg ctccatgttc
     6001 ttcggctatc tgcgcggaga ctggaagggc tatttcggca gcatgggcat ccgtcgggaa
     6061 gacggaaagg aataccgcat cgtccgcaat gaaaaagaat atttcctgtc agacggtcag
     6121 gaaaaagtac aggaagagcc agcgaatctg tggtggcacg gcctggaccg tcagacgtat
     6181 gatacgattt ttgccatggg cctggaagac ctgcagggct tcaaaatcct gtccaatgaa
     6241 gccgtgcgca gccatttctt cagcatcgaa ggcggcatcg gcatgggcct ggcccgcagt
     6301 gaagtgacca agcatatggg tgacctcctg gtcgcttcgc cgcagggcaa gaaacccatc
     6361 aatgccctgc tcaacgagca gaaggacttc gaccgccgca ttaccggcat ggcctacgat
     6421 gaagatgaat tcgccagcct gcaggaaacg gaacagacga cgcatgaaat cgagaaccgc
     6481 atccgcctgg atatcgaaga atcgaagcag cagatcgaac ggatttccat gcctatcgct
     6541 gcctgggatg tctataaacg cggccaggac gccatgcagc atatgcaggc cctggccgat
     6601 gtatcccagt tcccggctga cggcgcgcag aaatgggccg agctggaaca gaacatcaag
     6661 gccattgacg aacaggtccg caagctcaag gcgacgagcc gcaaaggccc ggccttccag
     6721 gaatcgtgga accgctggct ggcctgcggc ccccagctcg acgccatggc tcaccatgtc
     6781 ggtgaatgga agcaggggct ggaagagctt tcggaccatg aagataagga aatggactgg
     6841 gaatttgaag gcacgaaaca agccgacctg ctcaagtcct ggaccgatgg caaggatatt
     6901 ccggcagccg tcaactggac tcaggggatt tcggccgccg ctgccatcga ccacagtcgt
     6961 caggatttag agaaatggca ggctgacaag cccaagaatg tttccggtgc cagcggcgaa
     7021 gacggtccgg ctgaaaagac caaggacgaa tgggatgtca tcgggacggc cgtagcgacg
     7081 atccagaacg tcgtcatgga acgccagaag atacaggaac agctggaatg gctgaagaac
     7141 gaaccggctg gctcgtcgaa ggcctttacc ttcgtcggca tcgctttcct ggtcctggcg
     7201 gctctctgcg tcgccgctgt ctatctggct cacttcgatt cgaccctggg cctgggcggt
     7261 gccgctgcct gtgtcgtcat cgccatcatt tccctggtcc gccagagtac cggtgccgac
     7321 cgggttcccc ggaaaatcgc agaaatcgaa ggccagctgg caggcgtcaa ccgcaaaatc
     7381 agcgacttag ccaaagaagc ggaaatgacc ctcagtacgg acgaatcgaa cgaacgctgg
     7441 ctccaggaac tggacgccgt ccgcaagcag tatctggact ggcagacgcg ggaaacgaag
     7501 aatgcctggc agaaagaaca gaaagtcatg tacgacgcca tttatgaaaa atggcagtcc
     7561 gatggcaata cctgcaaaaa acagctcctg gcctgtgaaa cggcctggga cggctggcgc
     7621 gataaaaata gtttcagcaa actggatacg gccgatgtgg ccaaagccaa agaagcctgg
     7681 gatacgtggc atgaagtcac gacggccgct gccgattgga agcgacgcaa gttggagttg
     7741 aaaggcgcca tttcccgctg gagcgatacg gcagaacaga ttttccggga agtcggcgtc
     7801 aagaaggccg tttccccgga taccgtcgaa gacgtctaca aacagtggca ggacatccgc
     7861 gtccaggctg aagtggccaa agaacaggac cgtcagcaga aagaacggga aagtcagatt
     7921 gccagcctgg gagaagaacg gcagcagcgc gtccaagaac agcaggaact gctcaaggca
     7981 accggcgccc agacggaagg cgaattccgc agcaaagtcc tgcgcttccg ccagttccac
     8041 cagtacaaag aagtctatga ccagacggaa gcccatatcc gtctcatcgc caagacgccg
     8101 aaaaacctgg ccgaactgcg ccatgaattg aaaatccata ccttgaagac ctggacagat
     8161 gaacgcgact actaccagaa aaaaatcgcc gatgccgaaa agaaactggc tgaagtcgca
     8221 gaaaaacgag gcagtatcat tgaacgcctg agccagatgg ccaagagcga cgaatacggc
     8281 aagctcctgc aggccaaaca gaaccgcaag gccgaactgg accgcgacgt cgacgactgg
     8341 ctgaccaata tgtacaccca gtacatgctg gaacaggccc aggaatacta cgaacgggtc
     8401 cgccagccta tcgtcatccg ccaggccggc gaatacctca atctcatgac ccagggccgc
     8461 tataccctgc aggctagcct ggacggcaaa cagctctttg ccgtcgatgg cagccagcgc
     8521 cgcgtcccgg aaaaacagtg gagcagcggc ctgggcgacc agatttacct ggccatccgc
     8581 atcagcctgg ccatggcctt ctccaaacaa atcgaaccga tgccgctcat cctcgacgac
     8641 atcctcgtcc gcttcgacga acagcgccag aaagaagcca tccagttcct ggcctcgttg
     8701 ggcaaaaaag aacagatatt cctgttcacc tgctccgatg ccacgttgca cctggccgaa
     8761 gaagtccaaa aacaactgtc tggagaaaca gacacgatcc atctctttga aatcgaaaaa
     8821 gggacgatac ggggaaagga aggcttatgc tagagtatgt agttcgacaa acggcggcct
     8881 acatggaatc catgcctaaa cagattcgca aaaaaagtgg tcaattcttt accagcatag
     8941 aaacagcccg gttcatggcc gggctgtttg ctatccctgc acagcctgaa attaccatat
     9001 tggatcccgg agctggcacg ggtatcctta cggctgcttt attggaacgt atttcggcaa
     9061 tcccagctgt ggaacgagta caccttatat gttatgaaac agatgaaaaa atcattcctg
     9121 ttttagaaga taatcttcgt tatgtgaccg ctcattgttt aaaaaaagct gactatgagc
     9181 tccgcacttt gaattatatt acaagccagg aaaatgcttt caatggagtc ctgtttccag
     9241 aatcaggcat cacttgcgac ctatgtattg gcaacccgca gaatatgaac atcg
//
LOCUS       DAWCYS010000042         9159 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_490_length_9159_cov_6.140817, whole genome shotgun sequence.
ACCESSION   DAWCYS010000042 DAWCYS010000000
VERSION     DAWCYS010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_490_length_9159_cov_6.140817"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1750 a         2516 c         2730 g         2163 t
ORIGIN      
        1 atatagcccg agccgccgcc gatgagaccg caggcgactg ctgcagctat aatcgttagc
       61 atagtactgg attcacctcc cctttcacaa aaaaaagccg aactcgcagt ccggcttttt
      121 ttatgagtat gtaaaattta cttcatgttc aaaaaaccta caattatgca aaatatagac
      181 cagtgtatca aaccggctct cttaattgta aatttttttt atatcaatgt caagatagac
      241 ttagtcaaga ttaacgtatc aatggaccgt aaaatcttcc aggacgtccc gtatgactga
      301 gggcgaaaag ccgcggtact gtaaaaaacg ggccgccttg cgggcttcat cggcacgata
      361 agagaacttg cgcttcacca ggttcccggc cagggcgtat tcatcgggcc gctcgacgtc
      421 ttccggtacg ggcaggcagc ggcggcgcag gcggttgacg atatacaagt ggccgtactg
      481 ccctttgcgg gcataatagc ggtacacgtc acgggccagg cgctggtcgt cgaggaagtg
      541 ttccccttcc agagcagcca cgacgtcctt gaggacgtcg tcgtcaacac cgcggcggcg
      601 cagcttggcc ttcagctccc cttcgctgag aaagcggacg ttgaggagct gcaaggcttt
      661 ttcataagct tcgcgataat ccatttaggc ttcgtcgctg ttttcagcag ttgtatccgc
      721 cgcatccagg gacgccgtat cttcttcttc catggcttcg tcgaattttt ccggttctgc
      781 tgccagggta tcgcggatga tcttttccac ttcgtccgtg atttccggat gttccttcat
      841 gaaaatcttg acgttttcct tcccctggcc caggcgttca cccttatagg aataccaggt
      901 gccgctcttt tcgatgatat ccatagatgt acccaggtcg aggaccgtgc cttcgcggga
      961 aatgcccgtg ccgtacatga tgtcgaattc gcagtgcttg aaaggtggag ccactttatt
     1021 cttgacgact ttgacccgcg cccggttgcc gatgacatcg ccgccggact tgatggcttc
     1081 gcccttgcgg atttccatgc ggattgaagc gtagaatttc agggcccggc cgccagtcgt
     1141 cgtttccggg ttgccgaaca tgacgccgac tttttcgcgg agctggttga tgaagatgac
     1201 gatggttttg gacttggaaa tgatgcccgg caggttgcgc cgggcctggc tcatgaggcg
     1261 ggcctggagc ccgacgtggg aatcgcccat ttccccttcg atttccgctt tcgggaccag
     1321 ggcggctacg gagtcgacga cgatgatgtc gacggcgccg ctgcggacga gttcttccgt
     1381 gatttccaag gcctgttcgc cattatcggg ctgggaaatg agcagttcat tcgtgtcgac
     1441 gccgaggtgg cgggcataga ccggatccag ggcgtgttcg gcatcgatga aagcggcaat
     1501 gccgccgctc ttctgggccg aggcgatggc atgcagggcc agggtcgtct tgcctgacga
     1561 ttcaggaccg tagatttcga tgacgcggcc gcggggataa ccgccgacgc cgacggccag
     1621 gtcgacggcc agggaacccg atgagatgac ttccaggttc atcttgtcgg cgacgtcgcc
     1681 gaggcgcatg atggcgcctt tgccgaaatc cttttcaatc ttgcgcattg cattttcaag
     1741 tgctgtctgt tttgcgtcca aattgtctcc tcctaactat gctttctttg tatgtgtcac
     1801 tatatagtgg actaagtaat agacggcccg cagggcagcc cgctgtttga cttcgacccg
     1861 gccgccgtga tagacgttgc ggtgggtcac ggtcccccag ggaccgctga tacccgtata
     1921 gaccaggccg acgggctggt ccggcgtgcc gccgccgggg ccggcaatgc ccgtcgtact
     1981 gacggccagg tccgtcccgt acaggcggcg gctgccttcg gccatttcag cagccgtctg
     2041 tacgctgacg gccgtatagc gttccagcgt atcgtgagaa acgccgagta tcttttcttt
     2101 catttcattg cagtacgtcc cggccgagcc gcggaagtaa tcggaggcgc ccggcacatc
     2161 ggtgatgagt gagcccacca ggccgccggt acacgattcg gcagcgctga cggaatagcc
     2221 gctgtcgcga acgagctgac ccaggacatg ggccatggta tcgtcgtcga agctgacaat
     2281 ctggccagcc agccggcggc ggacttcccc ttccagggga gccagcaggg accgcgcctg
     2341 atcttcccgg tcagcccggg ccgtcaggcg gacttcgacg aagcctggcc gggcatacat
     2401 ggccagggtc ggattcgatt gctccttgac caggtccatg agtttctctt cgatgaaggc
     2461 ttcgcccatg tcatagattt tgatatagcg cgaagcgatg cagccctggc tgccaaaagt
     2521 ctggagcagc cacggtttca ggcggttttc atacatccag cgcatttccg ttggcggtcc
     2581 cggcaggtgg accagggtct tgccgccttg ctgcatggcc acacccggtg ccgtaccggc
     2641 gccgttatcg aagaggacgg tgccctgtgg gaccatggcc tggcgcttct ggttttccgt
     2701 catggccgtg ctgccgtgat ggacgaggac ccattggcgg accgattcgg cgatggtttc
     2761 attataaacg agaggcattc ccatgaggcg ggcgccgatg atcttggtca tatcgccctg
     2821 ggtcggcccg aggccgcctg tggtgatgac catgtcgacg cgggacaggg ccgtcttcag
     2881 ggcttcttcc atgcgccgcg ggttgtcgcc gaccgtcgta tgatagatga cagaatagcc
     2941 gatttcattg agtttcctcg acaagtaagc tgagttataa ttggtaattt cacctaacag
     3001 taattcggaa cctgtcgtaa ccagttccac gaacataagc gatcacctcg gttaatccat
     3061 ccgttccgct acgaggtcat aggcaaagcc ctgggcgatg cggacgggaa tcatatcgcc
     3121 aggctgtaca tcctgggcgt cttccagata ggtcaggcca tcgacgtcgg gagcctggat
     3181 ttcaatgcgc ccgacagcct ggcggatacc cttttcttct tctactcttt cgacgagcat
     3241 cgtatggaca gagccttcca gttcctgatt gcggatttcc gataccttgg cctggatggc
     3301 catgagctga tgataccgtt cttccttgac ttcttccgga atctggccgt ccatgagggc
     3361 tgccggcgtg ccgtcttcgc gggaataggt gaagacgccg acatcgtcga attccgtttc
     3421 cttgacgaaa tcacagagtt cctggaattc ttcttccgtt tcgccaggga agccgacgat
     3481 gaacgtcgag cgcagggtca gcttgcggcc gtgactgcag agcttcttca tgaggctgcg
     3541 cacatcggct tcggaatcgc ggcggttcat gcgctgcaag acggactggg aaatgtgctg
     3601 gagcggcaag tcgacgtagg gacagatttt ttcttccttg acgatgaggt ccatcaattc
     3661 atccgtaaag tagtggggat agagatagaa caggcggatc cagcggatgc cgtcgatctt
     3721 caccagctgg cgcagcagtt ccggcaacag ggaatgaccg gccaggtcga cgccatagct
     3781 cgtcgtgtcc tgggcgatga ggttgacttc cttgacgccg tcggctgcca gctgctgtac
     3841 ttcggcgacg acggaatcga cgggccggct gtacagcttg ccccgtacat ggggaatgat
     3901 gcagaacgag cagccgttgt tgcagccttc gccgattttc acataggcac tgtacttcgg
     3961 cgtcgtccgc aggcggctcg tattctggtt gtagagatgg ctgacatcgt ccatgaagca
     4021 ggctttgcgc cctttcttga ccgtatcgat ggcttcccag atgcggtccc acgaaccggt
     4081 tcccaagagg gcgtcgattt ccggcatttc cttcatcaaa tcttctttat actgctggct
     4141 caggcagccc gtgacgatga gtatcttgca tttcccctcc gtcttgtagc gggcagcctg
     4201 caatatcgta tcaatggatt cctgcttggc tttttcaatg aacgtacacg tattgacgat
     4261 aatgatatcc gcctggggca ggtcctctgt gatctccata tggcggtcgc ggaggatgcc
     4321 cagcatgact tccgtatcaa taaggttttt ggagcagcct aagctgacaa atccgatttt
     4381 ttccatgtat ctttactttt cctttccgaa gcgaattgat ttgatccacc aggcctgcaa
     4441 atcgcggcgg ccctgatcag aatactgttt ttttacgggg aataagacga gctcccggcc
     4501 tttggcatcg acttccgggt tggccggcgg cagggcttcg ctcaaaaaaa tgtgtctctg
     4561 ccgcaagacg tcaatagcat cgggcgaata gagccagtcg atgaaggcgt tggtcaattc
     4621 tccatcttca caccagttgg tgacagccgc accggtcagc cagtacgacg tgccgtcttg
     4681 aggatagagg atgaagatcg gcgccccgtc tttgtaatac tgccgggcga cggacgcgtc
     4741 ggcgacgccc atatcggctt cgccgctggc tacgcggcgg acattgaccg acatggactt
     4801 agaataggcc gctacatggc tctggagcga gctcagatag cgttcactgt cttccatgcc
     4861 tctcatttcc acaaaagaac agaggaactc gccggccata tccatggatg ccaggtccgg
     4921 gaaagccagg accatacccg ggtcggtcaa caggtcgtcc caggactgga tctgcatgcc
     4981 ccgccgttcg taataggcct ggctgaggac gaagaccata gggttatacc agaggccatt
     5041 ccagagctgt tccgggtcct tcatggacgg cggcacttta tccgtgaccg gtgaggcgta
     5101 gggctgcaag atgccgtttt tcttctgagc ctgcaagtcg tcttccgagg cgatgaggat
     5161 atcgggcttg acgccgccgt cgtccttcag ggactggcgg atctcgctgt cgctcttgga
     5221 atagatctgg acgcgcagat gtttctgttc gtaaaagcgc tgggccaggc cgtcgttgac
     5281 atcctgaggc aagtcggaaa agacgacgag atggcgttcg gcttcatacg cccgctgtgc
     5341 ttcttcctgc tggcgctgta ccaggatggg cccgcacagg cccaggagca ggatgacgaa
     5401 gaggctgagc acgataaagg gcacgaattt cttcattagc ttcacctgaa ttttctagtc
     5461 ttagccggtc ccctcccctg cctgcggggc ggagcggcat gccggccggc cttatcgggc
     5521 cgtctccctg cctgatggcg ccgcggcgcg atgaggcatt tgggaccgta tccgatgagg
     5581 tcccgtcggc cggccaggca cagggcctta tagacaatat cataattggc cgggttctta
     5641 tactgcatca gggcccgctg catctgtttc tcttccggcc ggcgggcgac gtagactttt
     5701 tcacccgtca agggattgag gcctgtatag tacatggccg tcgacaggct tccaggcgtc
     5761 gggatgaagt cctgtacctg ttccggctgc atatgcatat cccgaaggaa ttcggccagg
     5821 gcgacggcat ctttcagggt acagccggga tggctggaca tgaaatacgg gacgaggtac
     5881 tgtttcttcc ccagttcccg gttggcttca tcgaaccagt ccttgaactt caggaaggca
     5941 tagacgtcgg ccttgcccat gaggtccgtc acgtgcttga cgacgtgttc cggcgccact
     6001 ttcagctggc cgctgacgtg gtactgacag agttccttga cgaaagcctt gttgtggtcg
     6061 gccaggacgt agtcatagcg caggcctgag cggacgaaga ccttcttgac gcctttgacg
     6121 ctgcgtattt cccgcagcag cttcagataa tcgtcgtggg acgtattgag gttcggacac
     6181 gtttcgctgc cgatgcagtg gcggttgcgg caggccccgt cggtgagctg cttggcacag
     6241 gcgacgtggc ggaagttcgc cgtcgggccg ccgacatcgt ggatgtggcc tttgaaaccg
     6301 ggcagatggg tcatgcgctc cgtttccctg accagtgatt catggctgcg atgctggatg
     6361 atgcggccct gatggctggc aatggcgcag aaatggcagt ggccgaaaca gccgcgctgg
     6421 ctggtcaggc tgaactggac ctcatgcagg gccggaacgc cgccggctgc gtcgtaatca
     6481 ggatgccagc gccgctggaa gggcagttcg tagatagcat ccatctcggc tgtcgtcagc
     6541 ggcagggccg gcggcgtctg gacgacgaag cggttctctg acggctggat gatagtcttg
     6601 ccgtaaaagg gatcctgttc ttcgtactgc atcttgaaag ccttggcaaa ggtcaattta
     6661 tccttcttga cgtcttcata agacggacag acgacggtct tatctgaaat gggcggccgg
     6721 ctggtgacgt agcagatgcc gcggatgtcg tacatttcgg cgacggtctt gccttcggcc
     6781 agagcgtcgg cgatttcgac gacggaatgt tcccccatgc cgtagctgag gatgtcggcc
     6841 ttgctgtcca ggaggatgga atggcgcact ttattggacc agtaatcata gtgggcaaag
     6901 cggcgcaggc tggcttcgat gccgccgatg atgatgggca catccttata ggcttcccgc
     6961 atgcggttgg catagacgat ggtcgcccgt tccggccggt ggccggcttc gccgcctggc
     7021 gaatagctgt cgcggctgcg gaatttcttg gctgccgtct tttcattgag catggaatcg
     7081 agattgccgg ctgtaatcaa agcggccagc cgcggccgtc ccaatttctt gaacggttcg
     7141 gcactatgcc agtcgggatg ggaaatgata ccgacacgat agccccggct ttccaatacg
     7201 cggccgatga gggctgcggc aaaaccgggg tgatcgacat aggcgtcgcc agtgaggtag
     7261 ccaaaatcga gctgatccca gccgcggcgt tcgatgtcct cccggctcgt tactacgatt
     7321 gtctcgcgca gatggctcct tatttaaatt aaaatccaaa tgatgagcag caggaaaatg
     7381 atgatgccgg caatgatagt gatttccggc cattttccct gacgctgctt cttcttttta
     7441 ttgctgtcct tgcggacttt cttctttttg acgggcatga tggtccgcac ttccggctgc
     7501 ggcgtccgtt cttcgacgtt ttccttgtat tcccgggcca gggcgatgcc gtccagttcc
     7561 aggaaaatgc cgtaattgcg aagaaagccc ttgacgaaga cggcgccggg aatggcatcg
     7621 tattcgtcct tttccagggc ttccaggtat tcccgcttta tattgagggc atcggacacg
     7681 tcttgtagtg tcagcccttg ttgttctctt gccagtcgca gcacttctcc gatcgacgat
     7741 tcgttcactg ttgttcctct ccccattctc tataatcgta ccttttatta tataacagat
     7801 acggccaggg gtacaggggt tcagctcaaa tacttctgct ggacttcgtc ggccgtcatg
     7861 aggatttccc tgggcttgct gccgttattg gggccgacga tgtgcatggc ttccatcatg
     7921 tcgatcatgc gggctgcccg cgtatagccg acgcggaagc ggcgctggag catggacgtc
     7981 gaagcctggc ccgtttccag gaccatgtcg atggcctggc gcatgaggtc gtcttcaaag
     8041 aaatcgacgc tgtcttcggg attttccgac tgggccttct ggacggcttc atcgtaattg
     8101 ggaccgccct gggccttgat gaattcgacc atttcgtcga tttccgaatc ggaaatgaaa
     8161 gcgccctgga cgcggagagg cttggaagca ccgagaggat agaagagcat gtcgcccttg
     8221 ccaatcagtt tttctgcacc ggccatgtcg aggatagtcc gtgaatcgac ctggctggaa
     8281 acggcgaagg caatgcggct cgggacattg gccttgatga gacccgtcag gacatcgaca
     8341 gacggccgct gtgtcgccag gacgaggtgc atgccgcagg cacgggcttt ctggcccagg
     8401 cggatgatgg acttttccac gtcgtctgag gccatcatca tgaggtcggc caattcgtcg
     8461 atgatgatga cgatgaaagg catcgtgtct tccggatgga gttcattata gcgggaaata
     8521 tcccgcgtat gggtgatggc gaagcgctta tagcggtcgt ccatttcccg gacagcccag
     8581 cgcaggacgc tggcggcctt cttcgggtcc gtgacgactg gcgtcagcaa gtgcgggatg
     8641 ccgttgtaat tggataattc gacgaccttc gggtcgatga ggatgagctt gacgtcttcc
     8701 ggccgctgct tgaagaggat gctggcaatg aaggtgttga tgcagacact cttgccggac
     8761 ccggtcgagc cggctaccag gagatgaggc atcttggtca ggtcggcgac gatggggttg
     8821 ccggcaatgt ctttgcccag gcagaccggg acgccgccgg cggccttgcg gaacgtatcc
     8881 gtgtcgatga catcgcgcag attgacaccg gtcagctttt tattcggcac ttcgatgccg
     8941 acggccgctt tgccgggaat cggcgcttcg atgcggatat ccgtggcggc cagtttcaag
     9001 gcgatatcat cggctaagtg gacgattttg ctgactttga cgcccgctgc cggttccagc
     9061 tcgtaacggg tgatagacgg cccctggcag gcattgagta tcttggcatc gatgttgaag
     9121 ctctgcagct ttcctggagg atgcgggcag tttcccgga
//
LOCUS       DAWCYS010000043         9116 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_498_length_9116_cov_4.765258, whole genome shotgun sequence.
ACCESSION   DAWCYS010000043 DAWCYS010000000
VERSION     DAWCYS010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_9116_cov_4.765258"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2021 a         2477 c         2604 g         2014 t
ORIGIN      
        1 tcgcatcgat gacttcaagg acatgatcgt atccggcgaa tcggccatgt gcgtcgtctt
       61 ttccggtgac ggccggcgca tgatcgggga aaacaggaac ctggaatacg tcatccccaa
      121 agaagggacc aacctctgga ttgactcgtg ggtcatcccc aagaatgccg aacacgtcga
      181 aaatgcccat aaattcatcg acttcatgag ccgtcccgac atcgccctga agaacttcga
      241 gtacctgacc tattcgacgc cgaatacggc cgtcccggcc atggaagaag acgaagacta
      301 taaagataac cccgtcgtct tcccgccgga agaagcttat gccggccagg aaacgtacaa
      361 atacctgggc gaagaagggg acaagcgcta taacgacgaa tggatccagg tcaaggtctg
      421 gtgatttttt aggataaatg cttttcaaga atacaaatgt gtagtataat gagtacaagt
      481 tcagaatgag accaaagaag gaaccgttgc acgaaggctt ttgccatcat gcaaggctcc
      541 tttctcttta tagtttgtag tatggagttt gtagaaaatg gcttcaaatt taaaaacacc
      601 cactaaaaac tacaaactac aaacttcgaa cttctgttgt atgagattgg gaggcgggga
      661 tatggctgag aaaaggcctt tggttttcaa tgtgcataaa ttttccgtcc acgacgggcc
      721 ggggatagga acgacgattt tcttcttagg ctgtcccttg gcctgcccat ggtgccataa
      781 tccggagagt cagcgcttcg aggttgagag aatggagcgt agcgatgggg gacaggatga
      841 ggtcgggacc tattattccg tagcagaact cgtccgccag gccgtgcagg accagatttt
      901 ctatgaacgt tctggcggcg gcgtcacctt gtcgggcggg gaactcctgg cccagcccat
      961 ggggtatatc gccgatctcg tccgggccct ggctgaaaaa ggcatttccg tcggcatcga
     1021 caccagcggc gctgttccct ttacggcttt tgaaccggtc ctgacttata cggacttctt
     1081 cctttacgat atgaagctct tcgacgccga acgccaccga caatatatgg gccagggcaa
     1141 taggcagata ctggagaaca tgaagcgcct cagcgacgcc ggcgccggcc tctatctgcg
     1201 gctcatcatg gtcgacggcg tcaataccga cggggaccgg gatatccggc ccttccagga
     1261 ctggctccag gcgcagcaga tacgcgtgga aagaatccat ctcctgccgt accatgagct
     1321 gggacgccat aaatatgagg ccctgcatcg gacgttccag ccgttccatg ccccgtcagg
     1381 gcaggacttg gaagctatcc ggcagtcctt tgttgcactc ggttatgata ccgtcatcgg
     1441 cggttagata ggaggggatt catatggcaa cagctttgaa agaacagaaa ttcgtggaag
     1501 gtatccgggg atgtacggac cgtatccgcc ggctgcggga agaaagcgtt cacggcgccg
     1561 tcccgacgat ttccatggaa cgggccgtgc tgctgaccga agcgtaccag cagtacgaag
     1621 ggacttgttc cattcccgtc ctgcggggca aagcctttta ctatctcatg gcccatcgga
     1681 ccctctatct aggacggggc tcgctcatcg tcggcgaaaa ggggccgaaa ccctgggcgg
     1741 caccgacctt tccggaactg tgctgccata ccctggaaga cttcgacacc ctgaacagcc
     1801 gcgaacgggt cttcttcaga gtgaccgacg aagataagcg catccagcgg gacgtcatca
     1861 tcccatactg gaaggaccgg gccatgctga ccaagatgaa cggcctgctg ccccgggcat
     1921 ggcagaaatt atttgctgcc ggtatgttta cggaattcct gcagcagcgc ggtccgggcc
     1981 ataccgtggc cgatggcaag atatactgga agggctatgg cgatttcctg cgggacatcc
     2041 aggaacgcct ggccagcctc gatttcaata acgatatgga tgccctggaa aaacgggacg
     2101 aatgggaagg catggcctgg gtctgccggg gcatgatcct cttagggaaa cgctatgccg
     2161 ccctggcccg ggacatggcg gcgaaagaaa aagacccggc ctggcgccga gacctgctgg
     2221 atatcgccgc cgtctgcgac gtcgtcccgg aacaccggcc acagacgttc cgccaggcca
     2281 tccagatgta ctggttcacc catatcggcg tgacgacgga aatgaacaac tgggatgcct
     2341 attcgccagg caagttcgac cagtatctgg aacctttcta tgaccgggac ctggccgaag
     2401 ggcgcctgac ccgggacgat gccaaggagc tcctggaagc gctgtggatc cagttcaata
     2461 accagccggc accgccgaaa gtgggtatta ccctcaagga aagcgcgacg tataccgatt
     2521 tctgcaacat caataccggc gccctgcggt cccacgggac ctgaggcgtc agcgatctaa
     2581 gctatctcat cctggaagtc atggacgaca tgcgcctcct ccagccgagc tccaacgttc
     2641 agatttccca gaagacgccg gaacgcttcc tgcggaaggc tctggaaatc tcccgcaagg
     2701 gctggggcca gccggcgttc tataacagcg aagccatcgt ccaggaactg atgtatctgg
     2761 gcaaatccct cgatgatgcc cgggcctgcg gcattgccag cggctgcgtc gaagccggga
     2821 cggccgggaa agaagcctat gtcctgacgg gctatctcaa tatccccaag gtcctggaac
     2881 tggccctgca tcgcggctat gatgcctata ccggggaaaa ggcaggtatc gacgtcggcg
     2941 acccgttgca gtttgcgtca tatgacgatg tatggcaggc tttcctgaaa gaactgaaat
     3001 acgtcgtcga tgtcaaagtg gtcggcaata acctcatcga acggctctat atgcagtaca
     3061 tgcccgtgcc gctgctgtca gtcatcaccg acgactgcat tgccaatggg aaggactaca
     3121 atgccggcgg cgcccgctat aacacgagtt atatccagtg tgtcggcatt gcgaccatta
     3181 cagattccct ggccgtgctg aaacagcagg tctttgaaca gcggcggttc accatggaag
     3241 gcatcatcac cgcctgcgac cgggacttcg acggcgacga tgccttgctc gacgtcgtcc
     3301 tccatcatac gccgaagtac ggcaacgatg atgactacgc cgacgctatc ctccacgacg
     3361 tcgaccgggc tctgcaagca actattgccg gcaggccgac gccgaaaggc tcgaagacgg
     3421 tcgtcgaatt cctgccgacg acgtgtcacg tctactttgg ccaggtcatg ggcgccagcc
     3481 ctaacggccg ccgggcccat gtgcccctgc ccgatggtat ttcgccggaa aagggcgctg
     3541 atacccacgg accgacggcg gtcatcaagt cctgtgccaa actggaacag ctcaagactg
     3601 gcggcgctct gctcaatcag aaattcacgc cgtccgtcgt cgccggcccg aacggcctgg
     3661 cttgcctggc tgccctggtc cggtcgtact tcaccatgga cggccatcac atccagttca
     3721 acgtcgtcga cagggaaacc ctcctcgatg cccagcgcca cccggaacaa tacgaaaacc
     3781 tcatcgtccg cgtcgccggc tacagcgatt acttcaacaa cctggacaaa gccctgcagg
     3841 acgaaatcct caaccgcact taacagagct ttggggcgta aaaaagaccg tgcataatgg
     3901 tttctgccat catgcacggt ttcttttttt tgttttaaat tgttgtaaat ttttttcatt
     3961 gtaggggctc gcacgtggcg acccctacag tgccaaaagc gagttcacaa gattcgcggc
     4021 gggccttaga gccacgagcc acgaactact tgtcgaaggc tttcccagtg agcatttcgt
     4081 aggcttcttt gtatttggca atggtcttgt cgatgacgtc ctggggcagg aggtagtcgc
     4141 tgtccgggtt ggctttgagc cagtcacgga cgaactgttt gtcatacgac ggctggctct
     4201 ggccggcttt gtagccttcg aggggccaga agcgggagct gtccggcgtg agcatttcgt
     4261 cgcccaagac gatattgcct ttgtcatcga ggccgaattc aaacttcgta tcggcaatga
     4321 tgatgccttt ggagagggcg tattcagcac attttttata cagggccaag gtgtaatcgc
     4381 ggattttttc agcgtattcc ttgccttttc ccgggaaggt cttgtcgatg aaagcagcgc
     4441 cttcttcgag agagacgttt tcatcatggt cgccgagttc ggctttcgtg ctcggtgtga
     4501 agatcggttc cgggagtttt tctgattctt tgagacctgc cggcagtttg atgccgcaaa
     4561 cggtgccatt cttctgatag ctttcccagc cgctgccagt gatgtagcca cggacgatac
     4621 attccatggg aatcatagtc agtttctggc atttcatgct gttgccgatg aactgttcct
     4681 gctggaagaa ttccggcatg tcagcgttgt cgacggaaat catgtggttc gggatgatat
     4741 ccgacgtcat atcgaaccaa aacttggaca tctgcgtgag gatggcgccc tttttggtga
     4801 ttttgttctt aaggatgcgg tcgaaagcgg agatgcggtc cgttgcgacc atgataatgc
     4861 tgtcacctgc gtcatagact tcgcgtactt taccggattt aaaaggggtg tattctttca
     4921 tcttgtctgg cctccatgat tcagaactta ttttattatg gtagaacagt attatcatag
     4981 catattttgg ggaaagaaaa aaggttttgt catcaaagga caaaaccttt ttttcgtggc
     5041 tcgtagggga cgcccagagg gcgtcccgca aatcatgtgt gatgtgtata taataactct
     5101 tctggacggg cgccccacgt ggtcgcccct acaatgcggt ttgtgaggcc acgtggtcgt
     5161 gcttacgata cggtcgcggt ccggcttact gcagataata agtcaggctg acgctttggc
     5221 tgagggtgtt ggtgccggct tcgactggtg tcgagtcggc ttccatcttg ttgacggccc
     5281 gcaggctcat ggcagcgccg tagacgcggc tgtacgacgg gctcgtaccg gaaatgttga
     5341 tttccttgac ggcaccgagc tggctgccag cggcttcagc tgcggcctgg gcagcgcgct
     5401 gaccgttgtg gacggcttct ttgatcaggt tttccttgat ctggtcggcg tgttcattgg
     5461 taaaacggac gttgttgatc tggttggcac cgaggctgcc ggccttgttg atgacctgcg
     5521 ataccatagt gaggtcggtc agtttgactt ccaggctgtt ggtcaccgta tacgacgtga
     5581 ctttctggcc cttcatgtca tagttcggtg acatatagaa gcccgtggtc ttcatgtttt
     5641 ccgctgggat acccatggcc ttcaaggcat tcgtgacctg gttcatggcc agattattct
     5701 tggtccgggc ctgctgggca tcggcgtcgg tactggttgt gccgatagtg acataagccg
     5761 tatccggggc gacttcttgc tgggcatagc ccgtgacggt aacgacagac ggttccggac
     5821 cagctgccag ggcctggggc gcagccagga ccagggacga tgccagcata gcggcgacga
     5881 caaggccttt caagggttta tatttcataa gttaagacct cctttttaag atattcctat
     5941 tataccacgg cagggggatg tacgtctatg tgggataaaa tggcgaaatt gtctatgata
     6001 aatgttacga aagtgttgca ttttttttcg cggcgtacta taatagccac agatacatag
     6061 aaaaagctag gggagccttc gggctgagaa aagtgacgga ttacacttga ccctttacct
     6121 gatgcgggta atgccgccgt agggaagctt tacgggatta taaagctcac ctgcggagga
     6181 ccgccaggcg agcttttttt attgcattaa ggattatttt tatataggaa ggatgtttgt
     6241 aatggattac acaacacaga tggacgctgc ccgcaaaggc atcgtcacgc cgcagatgaa
     6301 gattgtcgct gaaaaggaac acatgcccgt tgaaaaactc atggccctcg tcgccagcgg
     6361 gaaagtcgcc attccggcca acaaagtcca taccagcctg tcgccggaag ctgtcggcca
     6421 ggactgccgc acgaaaatca atgtcaacct cggcgtttct ccggaacaca acaaccacga
     6481 agaagaactg cagaaagtcc agaatgccat cgacatgaaa gcagaagcta tcatggactt
     6541 gtccaatttc ggcaagaccc gtgacttccg ccgcaaactg gtcaagatgt ctacggccat
     6601 gatcggcacg gtccccatgt acgatgcggt cggcatgctg gaaaaagact tgaaagacat
     6661 taccgtcgat gaattcttct ccgtcgtcga acagcacgca gaagatggcg tcgacttcat
     6721 gaccatccac tgcggcatga accgcaagac ggcacagcgc atcaaagaca acccgcgcct
     6781 gaccaacgtc gtttcccgcg gcggttcgct gctcttcgcc tggatggaaa tgaacaacca
     6841 ggaaaacccg ttctacgaat attatgaccg tctcctcgac atctgtgaaa aatacgacgt
     6901 caccctcagc ctcggcgatg cctgccgtcc aggctgtacc catgacgcta ccgacgccgc
     6961 tcagatcgaa gaactcatca ccttgggcga attgaccaaa cgcgcctggg cccacaacgt
     7021 ccaggtcatg atcgaaggcc cgggccacat ggtcctcaac gaaatcgcag ccaacatgaa
     7081 gattgaaaaa cgcctgtgcc acggcgctcc gttctacgtc ctcgggccct tggtcacgga
     7141 catcgcaccg ggctatgacc acatcaccag cgccatcggt ggtgccatcg ccgcttcctg
     7201 cggtgccgac ttcctctgct atgtcacacc ggctgaacac ctgcgcctgc cgaccgtcga
     7261 cgacgtcaag gaaggcatca tcgctgcccg catcgcagcc cacgctgccg acttggtcaa
     7321 gggcatcccg ggcgctcagg aatgggatga cgccatgagc aaagcccgcg tcgacgtcga
     7381 cttcgacaag atgatttccc tgtccatcga cccggaaaag gcccgcagct attacgaatc
     7441 gagtaaaccg gaatgtgaag gcacctgcac catgtgtggt aagatgtgcc cggcccgtac
     7501 gatgaaacgc atcctcgccg gcgaagacgt ctccatccgc taggctccaa cctaaattta
     7561 cctcatagat actgaaaaag gcccgtcgcc agatgacggg cctttttcag gtacagaccg
     7621 ccgacctgta gtaggggctt ccacgtggga agcccgctag agagatttga ccacagccta
     7681 tacagttttg atggtatatt tttacgaatc acgaacgacg gcctttcctc ggattcagat
     7741 gcggcgttcc gccgcgacag actcatgatt gtattatgac catgtgctgt tcgtcataaa
     7801 ttccggttgt gtacagaatt cgcgcggcct ttttggcccg tcaggtagcc gaccagcgga
     7861 agctggacgg cttttagccg tcccctacgg tatcgcatac aggcgataaa attttatgtg
     7921 gctgtatgcc gcatttgtag gggctgtcct agtgggcagc ccgccagaga gatttcatca
     7981 cagcccatcc gggtataatg gcaggctttt tacgaaccac gaatcacgag ccacgaacca
     8041 cggccttttc cacgagccga gggtcactcg aggtgatgtt gttacgctga atctgttgcc
     8101 gcgcagcggc atctgagccc gaggtggcct gcgaacggcg gcctgcgggg ccgtcaggcc
     8161 ccaaccgcga tgaacgacga acgacctacg atgaacggtt ttatgccccg tcgtcaggcc
     8221 tcaggatttg tcaaagcatc tatgaactgg cgagctatgc tggacaggta gcggtgtttg
     8281 ggtgtgatga cggccagttg ggtgcgcagg cgcggttcgt cgatggcttt ggtctggagc
     8341 tggggcaggc gggccggcga gatggccgat gccgggacga cggcgtagcc caggccggtt
     8401 tcggcccaga gcagggtcgt ccgggcgtcg tcattgcggc agaggaggtt cggcgtgagg
     8461 ttgtgggctt cgcagacgtc gtggaagagc tggtcgaagc ggcggtagag gatgagcggc
     8521 aggccttgca gttgttctat ggtcagggtc ggctggcccg ggctggggtc ggccagggta
     8581 tcggcatagg ccaggaccat cggttcttca ggcagcaagg tgcagtcgaa ggcgtcctgc
     8641 ttgaagggcg tgcggacgat gccgatttcg atgagacctt tgcccagcat gtccaggacg
     8701 cggaaggtgt tgccgtcata gatttcaaag cggatgcccc gatgactggc gtggaattgg
     8761 cggagcgctg gccggagaat gacgctgccc gacgaggaca cggtcccgat ggacaaggtg
     8821 ccgcgcagac cattcttgaa atcggcgatt tcccgtcgtg tcgagtcggt gagggtaaga
     8881 atctgacggg cccggcggta gaggatttcg cccgcgtcgg tcaaggtcac gctgcgcggt
     8941 tcccgctgca ggagctggac gcccagttct tcttccagag ctttcatttg ctggctcaac
     9001 ggtggctgag ccatgtgcag gcgacgggct gccgccgtaa tctgaccttc ttcggcgatg
     9061 accatgaagt acgtaagttg acggatatcc atatcgatgc ctccttgaca tataat
//
LOCUS       DAWCYS010000044         9083 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_500_length_9083_cov_7.379375, whole genome shotgun sequence.
ACCESSION   DAWCYS010000044 DAWCYS010000000
VERSION     DAWCYS010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_9083_cov_7.379375"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2218..2227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2084 a         2417 c         2487 g         2085 t
ORIGIN      
        1 acttttctca cctgtccaat ggcactttac cgacaggcga tgtcctgata tagcttttcc
       61 cggatttaca ttttgatgtc gtgtgctgcc ttaattttta tccccatatc gccagtcaca
      121 gccgcgacgt catccgtcgc ctctataagg tcatggtgcc gggtgggacg ctgctcatca
      181 tgcacgatat gccccgcaag gccgtcaacg ccatccacgg cggccagccc gtactgccgc
      241 cggtcgatgt cctggaagag aaattgatca gcgccgggtt ttccgtcgtc atcgccatgg
      301 atacggaccg gttctatctc atcaaagtcg tcaaggaggc tgacgaagcc gatttcgtct
      361 acgacgacga ggaacagccg gccggcgtgg cgtcccatcc cctccatcac ggccatcacc
      421 atacggaaac gaagcgcgtc ctcaaccgcc tgtcgcgcat tacgggccac gtcgaagcca
      481 tcaagcgcat gatcgaagac ggccgcgact gcagcgacgt cctggtccag ctggccgccg
      541 tccattcggc cgtcgccggt gtgagccggg tcatcctcaa ggaccatatc gaccactgca
      601 tcatcgatgc catccgtgaa cacgacgcag atgccgtaga aaatctcaag aaagctatta
      661 atacctttgt caagtaatgg tataataaga ccagagtatc catttggaaa gggtgtgacc
      721 acgatgcagt ttcgtgtagt attaggtgat ttatcggagt ggaaggccga cgggatcgta
      781 tattctgcca gctcgaccct cttgccaacg aatagcgtca gcagtaagat tcatcagaaa
      841 ggcggcttgt ctttttccgc ctcgtgccgt acgatcggca tgtgccacgt cggcagtgct
      901 gtcatgacca agggttataa attgaaggcg cctttcatca tccacgctgt cgggccgtac
      961 tgggtaggcg gcaagcgcgg tgaagaaggg gaattgttgt cggcctataa gaaggccatc
     1021 catctggccg atgaaaagca tttgaagcac gtcgcttttg cttcgctgag tacggaagaa
     1081 aaacgctacc ccctgcagcg cgctgccgct gccgtcgtcc ccttgctcct gaccgacgga
     1141 accggcttcg accgcatcga catggtctgc acgagcccgg aagaacagga agcctatacg
     1201 aaagcggctg tcttcttctg gctccgccat ctcggagacg ctgccggcaa tgaacaggcg
     1261 gccatgatgg aagaagccgg cgctgccctg gccctgctgc agagccgcga tgatgcaccg
     1321 gacccgattg ccttgtcgga agaggtcaag aaaatcgaag ccatcgtcca tcctttcttg
     1381 aagttgagga aacgcagcct cgtagacatc gaacaggctg catcgcagat catggccttg
     1441 tatccggcca gcagtgcgga aggagtgtaa cccatggcaa aaagaaacgg aacccatgat
     1501 ctggacgtca ccgtccagtg cccggcctgt catgaatacg gcgtcttcca tacgtgggat
     1561 gtcatcgaca cggctgatgc gaaattgagc gaacgcgttc atttcgacga aaatctattt
     1621 ttctacacgt gccctcattg ccacgccaag atccacatgg aaagcaagtg cctctacatc
     1681 gaccgggaca agaagctcct ggtctggcac atcccggatc cgaaagaacg ggtcacgtcg
     1741 aaggaagtcc aggatgccct gggttcgccg tccttcacga cctatacctg ccgggctgcc
     1801 ctgacctggg gcgaatggcg ggaaaagatc atcgaactgg aaggctctta tgatgaccgc
     1861 ctctatgaaa ttatcaaata cggcgcttat cagctcctga gccaggaaga caaggaacgc
     1921 ctgcccctgg aagcctatca catcgactat gccgacgata gccacgatcc ggacaagctg
     1981 gccctggtct tcatgcagga agatgccaag ggtatggcct atgtctatcc cattacagaa
     2041 catttgaaag aagtgaccaa ggatatcttc ctgcccatca tcgaacagat cgccgatgcc
     2101 aaccgcaagg cccagttcga ccgtttcggc tattcctggg cccgccagtt cacgacgtac
     2161 aagggcgggc ccaggaagag acgacacggt cgaacagggc ctggccggtg gcagcggnnn
     2221 nnnnnnnaag aagacaagaa ccgcgaaacc tatggcaacc tcatcggttt ctgggtcaag
     2281 accattggcg acgaaatctt ccgggtcgaa gtgaagccgg aaaaataatg gtttgcggcg
     2341 ggccgccttg acaggacggc ccctacaagc cactagccac taaagaggct gtcgcatgac
     2401 gacggcctct ttttattgac agatgtttta attaaaaata taataaaaat aatgggttat
     2461 tgtaggattt gtattccctg gcaattcata attttgttag ataggaagga gacattggat
     2521 gttttctatt ttaaagcgta ttatgctgac gacggtcatt gctgtcggtt ttatgattgg
     2581 ctccatgacg gtgacggctc aggccgatat gccgccgacg attgctgaat cggcctgcat
     2641 gatcgacgtc gatacaggta aggtattata tcaggtctat ccgaccaagt gggtccaccc
     2701 ggcgagcacg acgaaaatcg tcaccctcat tacggccctg gaccaatata aggataagct
     2761 ggaccagccc ttgcagattt cctgggaagc tgccaatacg gaaccttcct gcctggggat
     2821 tgcaccgggc gatcccattt ctatccgtga agccctgcgg ggcatgatgg tcgtttccgg
     2881 gaacgatgcc gctgtcgccg tcgcccagac tttagggggt tctgtcgccg gctatgcgga
     2941 aaagatgaac caggaagcgg tcaaaatggg agctacccat acgaatttcg tcaatcccaa
     3001 cggcttgacg gcagcccatc accacacgac ggccatcgac atggccaaga tggctgcgta
     3061 cgggatgaag aacttccctg aatttcgtta tattgtagga ctcaaatcct atgatgtaaa
     3121 atacatggat ggtcgggaac cgaagcacgt cacgacgacg aaccatttcc tgaccagcgg
     3181 ctatcccggc gcgaacggcg tcaagacagg ctttaccaat gctgctggcg aatgtctcgt
     3241 tgcttcggca acgcgcgacg gccatacgct ggtcttggtc ctgtacaacg acgacaaccg
     3301 ctggaccgat gcaccgacca ttttagatta cggcttccgg agattgcaga cggggaaata
     3361 gcctgaccgg gagcgcaacg agggggtata gttatggcgc acgttttgat tattgaagat
     3421 gacagtatga tcgctgccat cgaacgggac tttcttgagg ccaacggctt tgaagtatcc
     3481 gtagccatga cgggaaagga aggcctgaag atctttgatg gcggagccat cgaagcagtc
     3541 ctgttggaca tcggcctgcc tgacacggat ggcttcctgt tgtgccgcaa actgcgggac
     3601 atcagcaacg tccccatcct tttcatcacc gctcggggga gcgacgacga taagatcaag
     3661 ggattgggac tcggtgctga cgattatatc gtcaagcctt tcaatccatc ggaaatgatg
     3721 gcccgcctga aggcccatct ggaaatccat cagagactgt taggggtacg tttccaggat
     3781 gccgatgaca acaaaccgga tatccaagtc ggcgacttgc gcattttcat caagcgccac
     3841 caggttttcc ggggaaaaac agaaatcaat ctgactggga aagaattcaa tctgctcttg
     3901 ttcctggccc gtcatcccaa ccaggtcttc tccaagaagt acctcttcga gatgatatgg
     3961 catctcgatg ccctgggcga actggcgacg gtgacggtcc acgtcaaccg cctgcgggac
     4021 aagctcaacg aagtcgaacc ggaattcacg gcaatcgaaa ccgtttgggg aaacggttat
     4081 cgcttccgcg tagaagaata aaaaaaatag gctgtcgcat atgcgacagc ctatttttta
     4141 ctgtaggggt cgccgcgtgg gcgacccgct ggatatttgg tgacaacctc tctggccgga
     4201 aaatcgctca tgttctgggg cgggcttgcc acgtgcaagc ccctacgaag ctagtcacca
     4261 gccagagctg ccagccagag ctactaacca cgagccacga accacgaacc actaaaaaag
     4321 gagctgtcgc aatgcgacag ctcctttttt cactattagt aattaacgtt gcggagttcg
     4381 aagaaggact ggggatgtgc acagaccggg cagactttcg gagcttcgac agcttctacg
     4441 atgaagccac agttgcggca gatccaggtc ttcttgtcgg atttcttgaa gacttcctgt
     4501 ttttcgacgt tagccaggag agcgaggtag cgttcttcgt ggtgtttttc gatttcagcg
     4561 actttttcaa ataagaaggc gattttgtcg aagccttctt cacgggcttc tttagcgaag
     4621 gtcgggtaca tttccgtcca ttcgtaatgt tcgccggcag cagcgtcttt caatgcgttg
     4681 atgtcgttgg gcagttcgcc gccgttgagg agtttgaacc agattttagc gtgttctttt
     4741 tcgttgttag ccgtttcttc aaagaaagct ttgatctgtt cgtagcctgc ttattttgca
     4801 acgcttgcaa agtaggtgta cttgttgcgg gcctgggatt cacctgcaaa ggcagcctgt
     4861 aagttttttt ctgttttcga accttttaat tccataattc atttcctcct tgataaaaaa
     4921 gtcatatttg aaattatttt ttagcacctc tttgggttga tttcattgta gcacttttta
     4981 actctgatgt caacggataa ttattattcg ttagtagaaa acttttaacg ggcccggttg
     5041 ttgtcgtggc cgtctgccgt atggtcccgg tcgccagcat ggctgctgtt ctgactgctg
     5101 ctgcggctgt tctggctctg gctgctccgg gaagacgacg actggctgct gcggctctgg
     5161 gattgggagc ggctgtggct ggtgctgtcg tcgcgggtat aggaatccca cttcgatttg
     5221 accgtgtcgc cggcatcttc ggccgagtcc ttgaggctgc cgtatttcgc cttgaactgg
     5281 gcttcaatct ggtccgcttt ttccgggtac atggccttca gtttagccag gagttcggcc
     5341 ttgctggcat ttcccttatc caaggcaccc tggatttctt tatactcttt ataataggcc
     5401 atccaatgct tcaacgggcc gtaatcgttg ctcatgaagg atgtgtagct gccgaaggcg
     5461 caggccagtg tcaggatgag gaagatgagg acgcgcttca tttactgcgc ccccttcccg
     5521 cgggctgcgg cgtggcaggc ctggctggcc gagctggccg tcatggcggc gaagaggacc
     5581 gtcaggacga cgaggacccg cgtcaccagg gagagcgtcg gcaagggcag gaggtccgta
     5641 taggcatagg ccgggatgaa ggaaatgccg atggctgcca ggatgacgat ggccagggcc
     5701 aggtaaagga aaccgctgag gataccgaag agggtcccca ggacgtccat gaaggggctg
     5761 gcgacggctt tggccgcttt gcccatctgg ctgtcgccgt cgttccagtt gaagtccttg
     5821 cccgtcttgg cggcttcggc ggcgtagcgc tggcgcatga cctgttcccg gtcgtaacgg
     5881 cgctgctgct gggccagctg ctgctgacag tagtattcgt cgtcccggtc ttggtcatag
     5941 aagacgcggt cttccacgcc cggcccgaaa tcgccgtagg cgccgtattc gttgccgggc
     6001 atacgcgggt tgtcttcggg gacgatgggt tcgccgatat aatagcggta aatgttcttg
     6061 ggatgtccca gtttacagca gatttcttct tccgacctgc cttctttggc accaaggcgg
     6121 aactgttctt cgcagtcttc gatgatgcct tttaaatcat ttttgtcgct cttgcgaaaa
     6181 tagtagcgca agaggtccat aaattcattc tttctcatgc tttactctgt caggcccttc
     6241 ctttcccgtg agtgcttcat agcgctggtg aaacgtcgcc ccgtactgcc aggcagcgac
     6301 gctgatggcg gcgatggcga tgatgacgat gaagatggca aataccggca tgtccggcaa
     6361 aaaggcgacc gatgccaggg gctctttttc cagggcgatg cagatggcca cccaggccag
     6421 gagcgcgatg gcgcagaccg tgcagatcgt gccgatgatg taacagctga gataggaccg
     6481 gcgcaggctc tgccggctca tcgatgattt caaagcaatt cctcctcttc aattcattac
     6541 ctgttatctt aacacaggca ctatagcagg tcaatcacgg cggcccgttc ccttgcgaag
     6601 gggggcctgg ggctatataa tgatatataa ctctatttgt cttttgaagg aggaattttg
     6661 atggctgatt ttgtccgtta ttatgtaggt gatgtcgttc agatgaagaa ggcccatccc
     6721 tgcggctgca atgaatggga agtgctgcgt atcggcgtcg atttcgtcat ccgctgcacc
     6781 gggtgcggcc accgggtcat gattcccagg ccaaaatttg aaaagtctgt aaagaaacga
     6841 ttaaaatgcg ctggtgatga aagtgaaaaa gcgtaaaaag ttagaaaaag gtatttttat
     6901 ttttaggact ttgtggtaca atattcaaca gccaatattt taatgcttta ctagaataaa
     6961 gaactaaaga aagggaggtc ataggatgaa caacaatacc ttggatggag tgaagatgct
     7021 ccaccatgat gaaatgcgca attatgaccg gatccagggg aaaatcgttt ctgtcattat
     7081 gaaccacggc tgtaagatta ttgaaacgcc gagttttgaa gattacgatg tataccagca
     7141 cttctttcca cagctccgcc agcagatggt caagaccatc gataccgatg gccgtgtcct
     7201 ggtcctgcgg ccggatgtga cgctgcctct cgtcgaaacg gcagcccgcg aattcccgcg
     7261 ctcgaaccag ctgctcaagt tcggttatgt cagtacagtc ttccgcgaat actacggccg
     7321 gtcgacgtat ggcaaggatt tcctgcaggg gggcattgaa atcctgggcg actccagccc
     7381 cgaatgtgac ggcgaagtca tcgtcacggc ggctgagatc ctcaaggccg tcggcgtcga
     7441 aaacatccgc atcgacatcg gtacggcagc ctatacgcag gcattgttcg atggccttcc
     7501 catttcggaa gaagagaaag aaacgctcaa aggctatatg gccgaccgca acctcgtggc
     7561 ctgcaagtcc tatatcgccg ccctgccggt atcggccgat gcccgcaagg ccctggaagc
     7621 cctgccggtc ctcttcgggc cctatgccca gaccctgagc aaggcccgag attactctat
     7681 caacagcggc atgctcaacg ccctgtcgcg gctggaacgg gtctatgact acatccttta
     7741 tgccggctat gccgataagg tccagctcga cttcggcttt gccagccgcc tgggctacta
     7801 tacggatacg gtcttcaagg tctacgtcga cggcgctctg tacgacatca tcgacggcgg
     7861 ccgctatgac cagctgtcgt cgcggttcgg cgtcgaccgc ccggcctgcg gcttcggcat
     7921 gaacatcaat ttattgtatg aatacatgaa cgatgccggc ctgttggaag atacggaacc
     7981 gtcgttccag atggccgtat cctatacagc cggcgaccag accctggtcc gggacctcat
     8041 gaactggcgc agccgcggct tccgcgtcgc cgcctatccc gattcgtcct tcatcgacag
     8101 cggcgattac agtatccacg ctatttaccg caacggctcg tactataagg acggcaaggc
     8161 catgacggcc gaagaattgg aagcactcat ggggaggttg taacatggcg attcatattg
     8221 cactggccaa gggccgtatt cataaagtgg tcatgaagta tttagccgaa agcggctatg
     8281 atttcccgac gtattccgaa gagagccgca agctcatctt tgaagacgcc aagggccagc
     8341 tcaaagtgac cctggtcaag tcacctgacg tcgctgtcta cgtcgaacgc ggcgccgctg
     8401 acgtcggtat cgtcggcaag gacgtcctca tcgaagaagc gccgggcgcc gacgtctacg
     8461 aagtcatgga cctgggcatc ggccgctgcc gcatggccgt cgccggtatc gccggcaaga
     8521 aggtcgacat gaccaagcgc atcactatcg gcacgaaata tccgcgcatt gcccgcagct
     8581 atttcgaggc caatcatcac agcgtcgaca tcatcaagct caacgggtcc gttgaactgg
     8641 caccgatcgt cggcttgtcc gatgtcatcg tcgatattgt cgaaacgggc aatactttga
     8701 aggccaacgg cctggaagtc ctgaaatact tcatggattt cagtgcccgc atgatttgca
     8761 atcccgtttc ctttaagatg aaatacaacg aaatccagca gctcatggat gtcatccgtg
     8821 aacacagcat gaaggcataa ctaaggggga gttagagtgg aatggataga tgcacccagt
     8881 gaagggttga ccttacgcga cattcgccgc attactaaac gggaccaggc agaaagtgaa
     8941 gacatccgca aggctgtcga agctatcctg gccgacatcg aacagcgcgg tgacgccgct
     9001 gtccgggaat atacgaaacg cttcgatggc gtcgacctcg ccagtttcct cgtgtcgccg
     9061 gaagaaatcg aagatgccgt cga
//
LOCUS       DAWCYS010000045         8981 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_508_length_8981_cov_7.228098, whole genome shotgun sequence.
ACCESSION   DAWCYS010000045 DAWCYS010000000
VERSION     DAWCYS010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_508_length_8981_cov_7.228098"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2116 a         2260 c         2316 g         2289 t
ORIGIN      
        1 acaccgctcc gacacatatg agtaccgtcc tcattccgga atttgaaacg aaaaaattat
       61 ggcaccgcct gcttcacaac cagagcggct ggctgctaag actccgtatc ctgccttact
      121 tagacgtcgt cgtatcgaca gtccccctgc agtttacgaa atagaaatca agtagcatct
      181 acaacaaagc cccgtcgaag gaaatttctt cgacggggct ttctgttatg gaactgtcat
      241 cgggagatgg tcagttattt tttatagagc atgcggaccg aattgaggac acacagggcg
      301 gatttgattt gttttctttt aagttgttct aataattggc tgaggaagtc actgatgtaa
      361 actgtccgga tggacttgtc cgtctttgga ttagagactt cagtacaacg agatgataca
      421 tatttcacag acctctgaat acagattttt ttctcatccc agtctatatc cgaccatctt
      481 tacggatttt gttgccgtcc ttatcttgac ctatatagac ccgaagcttc caatgaccat
      541 ttcctcgatt gattacagta gccaacaatt acacctccta aaaaagaggc tcacttagag
      601 agcctctttg atatgctacg atactataaa ttgtattttt attaccattt cattcataaa
      661 tcattgtccc tttttatttt ccataaaatc gcttaaccat gcggtttata ggccctttaa
      721 agcttgtatt ccataaatgt agagcttgta ttcagggtca aactgcaccc ctcctgcacc
      781 ccttttgaag aaaaatcact aaaaatcagc gaaaatgtac agtaaaacaa ttactcagaa
      841 aatcaataaa aaaggcggaa aaagctagat tttatctgct tttccgccta ttgttaacca
      901 ttataaaagg ccgtgaggcc ttattttacc agtatttcaa gaaaattctt cgaaaacctt
      961 tacaaaccgc atgaatactg gtaaaatgaa taagtaaaag ttcatgcggg tgtaattcag
     1021 tggtagaata cgagcttccc aagcttggta cgagggttcg atccccttca tccgctccag
     1081 aataaaaaaa gggctgtcgc atgtgcgaca gccttttttt gtaggggcgc ccatgtgggg
     1141 cgcccgctca cacaaatccg gttcgcaagt attatcactt taaatttaaa gcaacctctc
     1201 cggcctttca ggccacctcc cctataaggg gaggcaagag ctagtagcca gctactagcc
     1261 acgagccact aaccacgagc cacattccct acttagccgg catagccgcg ttttacgcct
     1321 tcacgggcat ctttttccca atcttcgaaa tgttctttag cagccttgcc aatctgttcg
     1381 tcgttgttgt aatcgccgag ttcggtctgg acttcatctt ccttggcttc ggaagcgttg
     1441 acggctgctg cgaaatcacg accgtattgg cggcatgttt cctgggttgc ctggtccggg
     1501 ttccactggc agcgcaggcc gtcgttgacg acgtcgaagc cggcaccctg gaggagagct
     1561 gtaatctgtt tgacagcttc accgctccag ccatagctgc cgaaagcagc ggctttctta
     1621 ttcttgaatt tcaggccgcg gatcatttcc atgatgccgc cgatggagaa gaggaagcca
     1681 tagttgatgg tcggcgagcc gacgaggatg gctttcgatt tgaagacttc cgtaacgatg
     1741 tcgttcttgt catcgacagc ggagttaaaa agtttcactg tcagcgtcgg atccgtttcg
     1801 cgcaggcctt cggcgatgca ttcagccatg cggcgcgtgg aattccacat cgtatcataa
     1861 atgatggtga tctggttttc ctggtaagct ttggcccaat cctgatattt ggcgacgatt
     1921 ttcaggggat ctttgcgcca gatgacaccg tggctcgggc aaatcatgtt gaccgggaca
     1981 cccatcttga ggatttcatc gattttccgc gtaaccatgg ggctgtagag gttgaggata
     2041 ttggcatagt atttcatggc ttcctgatag agttctgcct ggtctacgca gtcattgtac
     2101 attctttcgc tggcatagtg ctgaccgaaa ccgtcgttgc tgaagaggat gttttcgccg
     2161 gtcatataag tgaacatcgt atccggccaa tggagcatag cggcttcgat gaagacgagc
     2221 tggctgtcgc ccaggtccag ggtatcgccg gtcttgacgt tgacgaagtt ccaatcctgg
     2281 tgatagaggc cgcgcaggat ggcttcccct ttcttggtgc agtaaatcgg cgtatccggg
     2341 atttcccgca tcagtgccgg caaagcgccg ctgtggtcgt tttcattgtg gttcatgacg
     2401 atatagtcga tgtctttgag gtcgatttcc ttcttcaggt tagcgacgaa ttcgtcgtca
     2461 aagggtttcc agaccgtatc catgaggacc gtctttttat cgcggatcaa gtaggagttg
     2521 tacgaagagc ccttttctgt cgaatattca tggccgtgga attcgttcaa ttcccagtcg
     2581 attttaccta cccaagtcac tttttctgta atttttttac ccatgatgat cgtctccttt
     2641 tcagttatct ctatatgata aaattattct aacgtaagca gcaatgccat tttaaagggt
     2701 ccatcggcag tgatggcatg gcggccgttc ttggcaaaag caaagttttc gccggccttg
     2761 atttcatgat cttcgccttc atagcggatg acggctttgc cttccagggc aaaaatcaag
     2821 gcttcaccgg gagcagcgtg ttccgaaaga cctgtgccgg cgccgaagct catgacgacg
     2881 aatttgactt taggcgactg gataatgtcg cggttgatga tcttaccatc ctgataaggc
     2941 acgaggcctg ccaaagagaa aatcttgcct gcatcgatgt tcataaggaa ttcctcctct
     3001 actgtaagtt ccgtatagac cgccccatcg cggctcttca tgccaacggg tacggacggc
     3061 ggtgcgatga tggcctgacc agccaccaga ttatgttctt cccccggcca gtgaacggtc
     3121 agcgtaccgt cagcgacata gaggagtttc gcctgcctgt agatttccgg actgatgtcc
     3181 gtaccggcag ccagggaaaa gtaaaagact tgatactggg cttcattgac cagttcttta
     3241 gaaatcgtac agccggccac tggggcatta tcacggctga tagaaaagat atggcctttc
     3301 gtttctttca ttccactcac ctcgtttttt ctttcccttc gatgtgttaa gtatacgttt
     3361 ttttcatcat tatgtctgtt gtaattacaa cagataatag aatttctcaa ttataaaaaa
     3421 aataggcgtc gcattaagcg atgcctattt ttagcagcca gcagtcagcg gttagcagat
     3481 agctttaaat caacctctca gaccgccttc ggcggccagc tcccctatta tgggagcctt
     3541 gaggactgcc tctacgagct gctagccact aactgctagc cactagccac taaaaaaaga
     3601 cctgccgtgt atgcggtagg tctttttttc atcctcactg gatttcaatg gtgttggtcg
     3661 gttgtgcctg ttgggcatct ttcgggagag ttacggtcag gacgccatct ttgaaagcag
     3721 cctggatacc ttctttctga atacctgtga cagggaactg acggcagaag gacgaactcg
     3781 aacgttcttt gcagatgtag ttgtgctgtt cgtcttgcga atccttctgt tcgttgtggc
     3841 tggcagccaa agtcaggacg ttgttgtcgt agctcagatg gatgtcttct ttctggacgc
     3901 ccggcagatc agcggtcata acgtattcgc tgcctttgtc ttcaatgtcg actttcggca
     3961 tcgtgctgga gaattcactc ggccacatga agcgattgaa gaagttatca aaaacatcgg
     4021 gaacggaaaa ttcattatca acaactggga ataagctatt cataagtcat gccttctttt
     4081 tacgtaagtt tgaacaccat atatcaaaca ttgaatacaa gggagtttgg cgggcgcccc
     4141 acgtgggcgc ccctacaatt atttgacttc aatgcggtgg gcttcagcca ctttcttcgg
     4201 gtcttctttc ggcagggtta cggtcaggac gccgtcttta aaggctgcct ggatgccatc
     4261 tttctggatg ttgcggaccg tgaactgacg gcagaacgta tggctgctgc gttctttgcg
     4321 gatatatttc ttcttatcgt ctttttcatc tttcttttct tcgtgtttag ccgtcaaagt
     4381 caggatgtca tcgttgtagc tgacgttgat gtcttctttg gcaatgcccg gcaggtccgt
     4441 cgtcaggatg taggccttat cggtatcttc gatatctacc tgcgggacgc ggtagttgcc
     4501 ttctgtcgga ttttcaaagc tgttgaagaa gttgtcaaaa atcgtatcag ggaatacaaa
     4561 atcactgttc gttacaactg gaaataaacc tttcatcata atcattaccc tctttcaaca
     4621 catcgttcat tgttgctatg agtgaacctt cttttcagtt ctgttctttt gacctttttg
     4681 cttatctctc tgtaacctct tcctgattac agctatatca tagaccctat tagcactctt
     4741 gtcaagtgag tgctaataaa aaatttcaaa aaaagtgctg tcgcgtaaac gacagcactt
     4801 tttagtggtt agcagctagc cgttaggggc taggaaaaga aacctctccg ccctttcagg
     4861 ctacctcccc tgttaaggga ggcaaagaac gcggtctgcg gtctgcgaac ggcggcctgc
     4921 gaacggcggc ctgcgaacgg cgggccgccc tttgggacgg cccctacgag ccactaaatt
     4981 acttatcctg cgggacgagt tgtttgcccg gttttacgaa gatccaggct acgatggcga
     5041 tgatgccgaa cagggacgtt gccgtgaagg cgttgttcca gccgtagttg gtgacgaaga
     5101 aggccgtgac gataggagcc aggacgccgc cgatgttgcc ccagaggttg atccagccgg
     5161 aaacggaacc ggtgtattta ccacccaggg agatgacgga agaccagctg gcgctcatag
     5221 caaagcccaa ggcaccgagg gaaacggaca tccagaagat gttcatggcc gggtcagccg
     5281 tatgggatgc gatgtacagg ccgagggatg taacggcgac gccggccata gccgtgacgg
     5341 tgcgcatctt attctgcatt tcgctgtggc tgccagtggc aatcttgtcg gaaacgaagg
     5401 cggccaggaa gaccatagcc atcagtgcga tccaagggca ggaagcccag aagcccatgg
     5461 atttcaagga catgttgtga acttcagtca ggtacatggg cagccatgcc aggaagacgt
     5521 acatgatgta gtcgacgacc atgaactgga tgccgacagc ccagaactga gtcgagcgga
     5581 ggaacttatg ccagggagcg acggctttct tcagggagtc agccgaacgg ccttcgttga
     5641 tgtatgcagc ttcttcagca ttgacatagg gactgtctgc cggattgtcg caggcgaagc
     5701 ggtgccatac ccaggccagg gcgacgccga cgagaccgaa gatgatgaag acggcgtgcc
     5761 agccgaagag ccccatgaga gcgaccgttg cgaccgggcc gacgacgggg ccgaagaagg
     5821 tccctaacag gatggacgag ctggccttgc ctttttcgtg tttattgaac catgtaaagg
     5881 tagcggcccc taaggacgga aagacagggc cttcgccgat gccgaaagcc aggcggacag
     5941 cggcgaagct gatcttgcct ttagctaaag ccgtcaaggc cgtgaagacg gaccaccaga
     6001 ggatggccaa agccccggtc ttgcgcatac cgaacttttc agccaaaata ccgccaggga
     6061 cctgcatcaa agcgtagctg gcgaagaaac aggtctgaat gaagcccatg tcgattttcg
     6121 tgaagccgaa ttcctgcatg atgaccggcg tcgcgacgga aaggttgacg cggtccatgt
     6181 aagcgacgaa actgattaag aagattaaaa acgcgatttt ccatcggaag tttgtcatcg
     6241 tgccggcaac agcccgcgtt tcagctgcag cggtcatgat cttacccatt gctttttcca
     6301 tagccaatac ctctttacat gaataccaaa atacccaaga aataatttaa taatgaatac
     6361 tgtacgaggc acactcagag gatataaaaa acccctgctg ctccagtgga gcaacagggg
     6421 cgatgattcg cggtaccacc ctgtcattat actcaacgtc tttgacagac atcctataac
     6481 gcgggattgc gtctgtacct caattcccta gaaaaacagt cagtacagaa actcccgggc
     6541 gaagtcggac gattgcacct cgccggattc acagcaccgc cggctctctg aagaggatca
     6601 ggattcgtct tttacccgtt catcgtcatt atatccgcac tgctttcaca gtgctctgtt
     6661 tgtgtgatgt gcctattatc cctgaaaaga ggctggttgt caaggacttt tgttaatatt
     6721 tttaaatttt ctttcgttaa acgttatatt ttcaatttag ttaaacgttt ttattaccaa
     6781 ttttccagaa agactttatt ttttgtaaag catgcgtacc gaattgagga cacaaagggc
     6841 ggcgacgccg gaatcggcaa agacggcagc ccacatggaa gcataaccgg caaacccggc
     6901 aatcatgacg acgcctttga cgcctaaggc aaagatgacg ttttccatgg caatcttcat
     6961 ggtttcccgg gacaggtgga gtgctgccgg tacggcgctg acccgggact gcatgaagac
     7021 gacgtcagcc gcttcgacgg ctgcatcggc accactgccc atagcagcgc cgacatcggc
     7081 accggccagg acaggtgcat cgttgatgcc gtcgccgacg aagaggaccg gcccgtattg
     7141 cttacgcagg gcatccatct gattgacttt atcctgcggc agcagcttgg cccggacgtc
     7201 gtcgatgccc gtcttagagg cgatgtaatc tgcggctttc tgagaatcac cggtaagcat
     7261 ggccgtcgtg atgcccatgc gggaaatagc cttgacggcg tcgacagcgt cttctttgat
     7321 ggtgtccgaa atgaccaagc ggccggcata ggccccatcg atggcgacgt acagttccga
     7381 gccgtactcg acgtcgtcca tggccggcag gacgatgtgg cgggtatcca tgagctggcg
     7441 gttgccgcag aggacgctat ggctgccgac ctgggctttc aggccctgcc cggcgatttc
     7501 ttccatttcc gacggctcgg tcaggctcag gccctgttca caggcctggg cgacgacact
     7561 gcgggcaatg ggatggttcg acgcctgttc tgccgacgcg cagaggcaga ggacgtcatc
     7621 ggcactgcgg ttatcggccg gaacgacctg gtggacgacg aagttacctt tggtaatggt
     7681 accggtctta tcgaagacga tggcgccgat gtgcttcatc tgttccatgg caataccgcc
     7741 cttgaagagg atgccctgtt tcgacgccgc accgataccg gcgaagaagc tcaggggtac
     7801 gctgaggacc agggcgcagg ggcagctgat gaccaggaaa gtcaaagccg tataaatcca
     7861 gtagtgccac tgaccggtca ccagggacgg gatgatggcc gtagccgcgg cgatggcgac
     7921 gacagccggt gtatagatgc gggaaaagcg cgtaatgaaa cggtcgattt tcggcttgcg
     7981 cgccgctgcc cgttcgacgg attccaaaat cttcgtgacc atcgattctg ccaggggctt
     8041 ggtcactttg acgtggagga cgccagacgt attgatgctg cccgacagga cttccgagcc
     8101 ttcccggcag atgaccggaa ccggttcccc tgtcagggcc gacgtatcga tgaggctttc
     8161 gccggccatg acgacaccgt ccaaggggat gcggtcaccg acgcggacga cgacgacatc
     8221 accggcagca gcgtccttgg ccgggatggt gaccgtcgag ccatcggccg ttacgcgatg
     8281 gacgacttcc gggcgcatat cgatggcggc ggccacctgc ttgcggctgc ggttgacggc
     8341 ccggtcttcc atggcttcgc cgatgcggct gaagagcatg acggcgacgg cttctggata
     8401 gtcaccgatg gcaaaggcgc cgatggtagc cacggccatg agaaaatttt catcgaaagg
     8461 ctggcgcttc atgatgtttt ctcccgcttc ccgcagaacg ccgtagccta agagcagata
     8521 ggcgatgata taggcgggaa gggaaaaatc atcgggaatg atgccggcca ggtaggtgac
     8581 taaaaagagc accgtcccgg cgacgagggt ccacaaggga ctttcttctt cgtcatcatc
     8641 gtcgtcaccg cagcagccgc aagtgccgac agcatccatt ttgacggctt cgtcttcgtc
     8701 gtgatggccg ttgcagcagg aacagccctg gtctttcaaa aattcttctg ccatagatat
     8761 gcctccttca gggactcact ttttattcgc cttcattata ccattaaagt taaatatgtt
     8821 caagcgttca tatgttatct acacatactt tttaggcata ccctccccta tttgccgttt
     8881 atccagccct gtgcgataat aaggtgtact ttattatcga aacagtggtt gatactatga
     8941 aaataaagat tacaggcatc ggcggcgtcg ggggatatat a
//
LOCUS       DAWCYS010000046         8877 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_516_length_8877_cov_6.895715, whole genome shotgun sequence.
ACCESSION   DAWCYS010000046 DAWCYS010000000
VERSION     DAWCYS010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_8877_cov_6.895715"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2164 a         2388 c         2283 g         2042 t
ORIGIN      
        1 acggggactc ttccacgacg acgattacgt cgtctgcgca ccggcaccga cgccgcgctc
       61 agccgacccc aaggccatgg cggccatcct gccgtgcaaa gccgattggg ccggcaccat
      121 cgccgaaggc ctggacaaag ccctggctgc catcaaaccg gaccaggtcc tctgcatcgt
      181 cggctctctg tacatccagg gagaagtccg gaaatacttc cggaagagat accatattga
      241 gtaagggctg agcggctagc agcttgtagg ggccgtccca aagggcggcc cgcttgattc
      301 ctctttatag cagctagcgg tgagccacta gccattgaaa aggcgctgtc ttacaggaca
      361 gcgccttttc gttgcgcctc tgcgcctttt tatggtaaaa tgatacatat attttacacg
      421 gaatgtaagg agcgaacatt atgaagtgga tgatacggac cggcagtacg ccagccgagc
      481 gggcacggct gcgcgtcgtc tatgctatgg ctggtactgt cttttttatg ccggtgaact
      541 tgttcattat ggaaggcttt ttcatcatcg ccctgggcct ggggctctat tactgtaaaa
      601 aatatcccaa actgcccctg cgccagtcgc cgttgtccct gccggctgtc ggctttgccc
      661 tggtggcctt tctgtctctc gtcggctcgc cgcactttct cttagggacg gctttctatg
      721 ccttcacggt tttgcagtac ctcgtgctct actttggcat cctcctgttc gtccgccatt
      781 cctgggaacg gcgcctgctc ttttcgacgc tcctcctcag tgccttcgtc gtcgccctgt
      841 acggcctgta ccagtatgcc cacatgctga ccctccacga ggccgaatgg gtcgacaact
      901 cagcctttcc catgctgcgg cgccgcatgt actcgacgct gtacaaccct aacttattgt
      961 cggccttcct gctgattatc atgagcgctg cggcgtcgat gatgatctgt acccgccatc
     1021 gctggcatca cgtcatgtac ctggcctttt ttgccatcct gaccctctgc ctgatcctga
     1081 cctattcccg tggcgcatgg ctcagcgtct gcgctttgat cttctttttc ggcctctttt
     1141 gggataaacg ggtgtggctc ctctttctgg caggcccgct gatcctggct ttctaccacg
     1201 gcggcgtcgc cgaccgcctg atgtccattt tcagccacag cgaagcggac acgtccgttt
     1261 ccatgcgcat ggacatgtgg gaggcagcca ttgccatgtt tgtcgaccat accgtcctgg
     1321 gcattggctg gggcgccttc aaagacgtct atcccgtcta taatgaattg atccaggaag
     1381 caggtatcgt catcttccac gcccataata tgtacctcaa catcctggca gaaacgggca
     1441 tggccggcct gggcttcggc ttatggttct ttttcggcaa tgcctggtat gccatccgct
     1501 atgtacgcag ccatgcggcc cacaccttca gccgatccct ggccatgacc ctggccgctg
     1561 ccgtcttgtc cctgggaatc agcggcatga gcgactacga tttatttagt acccagattt
     1621 cacttacttt ttggctgatg agtgcccttt ttgctaacat gtatggcgaa gaatgtgaaa
     1681 aaaaacgata aaaatagttt gcgaaataat tcacaatgat atataataag tctgtgacta
     1741 ttatcactag ataagatgac ttatagctat agggaatagc ttgggacata gttggagggt
     1801 attatccatg agacagaaaa aggcaatatc ggatgctgtc atcgaacgat tgccgctgta
     1861 ctacagggac ttgctattat tgcaagacgc gggaatcgat atcatctcat ccgaaaagtt
     1921 aggggaacgg ttggggatta cgccggaaca aatccgcaaa gacctgacct gctttggggc
     1981 ttttggaaaa aagggcgtag gctattacgt cagtgaatta tgccagcaga tcgtggaaat
     2041 catcggcctg cagcagcatt ggaaaatcgg tatcgccggc atcggccatc tcggctgggc
     2101 cttggcgaac tacaagactt tcggacgctt gggcttccgc acgacaggct tgttcgacgt
     2161 cgatcaccgc atcatcggtc agaacgtcgg cggcgtcgaa gtcacgccga tttctgaaat
     2221 gggcgaagtc attcagcgcg aaggcatcca gatcggtatc atcacgacac cggcagctgt
     2281 agctcagcag gtcgccgacc agatggtcgc cgccggcatc cggggcatct tgtcctttgc
     2341 accgctgcgc ctgaccgtac cggacggcat cttcgtcttc cgcgaagacc tgtccatcgg
     2401 cttgagccgc atttcgtact atttgacgaa taagaaggat tgatgatggc cgtccgctat
     2461 tggcatggcg ggcgccctta cattatcttt atagtttgta gtacagatag tttttggggt
     2521 gtaggggtcg ccacgtgcga gcccctacag caaaaaggcg ctgtcttcat gcgacagcgc
     2581 cttttgtgtg ctataatatt cgtacattcc cgataatttg tagaatgagg tgatactact
     2641 atggattcta tctatggttc ctatggaccg gctttgcagg ctgctctgga agatattatc
     2701 ggccgtatcc gcgcctatag tgacgatata aaggccaaga ccggggaagc ggcttatgaa
     2761 cacctgattt accgcatcaa gtccgaaggg agtatgcggg aaaaatgtga ccgcaagggc
     2821 ctgcctcaga cggcctattc ggctctccac gatatccgcg atgccatcgg catccgtatc
     2881 gtctgttgtt tcctcgacga catctatcgg gctatcgact tcctgaaaag cctcgatggc
     2941 gtccgcgtcg tcgaagagaa agattatatc cgccacgtca aaccgaacgg ctaccgcagt
     3001 taccacgtca ttttggaact ggaagagcct tatgaagatg tgaccggcgc caatccgggt
     3061 catttctatg ccgaagtcca gctgcgcacg attgccatgg attcctgggc cagcctggaa
     3121 catcagatga aatacaagca cgacatcaag aacccggaac tcatcgtccg ggaactcaag
     3181 cgctgtgccg atgaactggc ttcgtgcgac gtgtccatgc agactatccg caatttgatc
     3241 catgcaaaag aataaggggg aaacgctatg cgccttctat tagctgaaga tgaaaaagaa
     3301 atgtccgcag ctctgtctgc catcctgacc cattcgggat acgatgtcga tgccgtctat
     3361 gacggccagg ctgccgtcga aaaagccctg tcccagtcct atgactgtat gatcttcgac
     3421 atcatgatgc cgaagaagga cggcgtccag gccttgaagg aaatccgcga caaagggaat
     3481 atgacgccgg tcatcatgct gacggccaaa gccgaaatcg acgaccgcat taccggcctc
     3541 gacgccggtg ccgacgacta cttgaccaag cccttcgcca tgggcgaact cctggcccgc
     3601 ctgcgctccc tgacgcggcg gtcgacgtcc tatgcgccgt cgaaactcac tgtcggcaat
     3661 gtcgaactcg acgtggaaga acaggaactg gcctgccgca attctatccg cctggccaat
     3721 aaggaaacga agctcatgaa attcttcatg gccaacgccg gcaaaaccct gtcgacggcc
     3781 cagatttttg ataacgtctg gggcgatgaa gaagacgacg tcgatgaagg catcgtcttc
     3841 ctttacgtct cgtatttacg ggaaaagctc gaagccgtcc aggccgacat tgaaatcgtc
     3901 ggccaaagag ggcaggacta tgccttgacc gtgaaagaag cgtctgcgga gggttagacc
     3961 atgaatatga tcaagcgcct gcgccgtaaa ttcatcatca tggcgacaat cggcgttctc
     4021 gtcgtcctcg gcggcgccct gggcctcatc aataccatcg cctatatgcg gatgaatcag
     4081 caggtcatgt cggtcctgac ctatatcatc cataacaacg gcgctgtgcc ccagaaaatc
     4141 ggcggcgacg atggcagttt cttcagcgac ccgtcatgga ccgaagagac gccagaattt
     4201 gcctaccaga tacgcttttt cagcgtcctc gtcaatgccg gcggctatgc ggaaaaaatc
     4261 gacatccagc acatcgcgtc gttttctgaa aaagaagcca tcgaatacgc ccggacgact
     4321 gtcgctgagg cgcgggaaaa cggaacgaaa cagggctttt tcaaaaagaa ccgggccagt
     4381 tatgcctata tgattcagcc caacgacgac ggcagcttcc tcatcgtcat cctggactgc
     4441 acgcgcgacg tcgctgctgt cacttcgttc atgcgctatt ctctctggtt tggcctgggc
     4501 tgtgtcatct tatacgtcat catcctggcg gccatgtgca acgtcgctat ccggcctttc
     4561 gtccgcaaca tggagaacca gaagcgcttc atcaccaatg ccgggcacga actcaagacg
     4621 ccgattgcca tcatctccgc caatgccgaa gccatcgaga tgctcaatgg ccagagccag
     4681 tggacggaca gcatcctcaa gcaggtccgc cgcctgtcca acctcatcca gaacctcatc
     4741 atgctgtcca agatgggcga acgctctcag gtcgacctgg tcattaccga tgtgaacttg
     4801 tcggaaacgg tccatgccgt agccgactcc ttccagcagc tggccattga cgccgaaaag
     4861 accttgcata cggatattgc cgacggcatc cacgtcaaag gcgacagtaa gtgcctctat
     4921 gaactggtca acatcctcgt cgacaacgct gtcaagtatt gcgatgacaa ggggaccatc
     4981 gacgtctccc tgcgccgtcc ccgtctgggg aagggggcga ttgtcgccgt gaccaacacc
     5041 tatgccgacg gggctaatgt ggattattcc cgcttctttg aacgcttcta ccgcggcgat
     5101 acgtcccata acagccagaa ggccggctat ggcatcggct tgtccatggc cgaagaattg
     5161 acgaaactca tgggcggtaa gattaatgta tcctataagg cccaacgtat aacgtttact
     5221 gttcggctgg gctgattttc cctataggga aagcgcctga cctgtgttat aatgtgacca
     5281 acatcacttt tattcagggg gaagatacca atggaaatgc acatggaata tatccgaaaa
     5341 ttaccggaac cgcaggaatt gatggaacaa tttcccatcg atgacaaggt caaggccatc
     5401 aaggcgaaac gggatgaaga aatcaaaaac atcctgaccg gccaagacga ccgcttcctg
     5461 ctcatcatcg gtccctgttc ggccgacaat gaaccggctg tcctcgacta tatccatcgc
     5521 ctggtcaagg tccaggaaca ggtcaaagat aaaatcttta tcatcccgcg cgtctacacc
     5581 agcaaacccc gtaccatcgg cgtcggctat aagggcatgc ttcatcagcc ggacccggaa
     5641 ggcaaggaag acatgctggg cggcatcatc gccatccgtg aaatgaatgc ccgcgtcgtc
     5701 cgcgaaacgg gctttacctg cgctgaagaa gtcctttatc cggaaatctt ccgttacttg
     5761 tcggacctct tgtcctatgc cgccgtcggc gcccggtccg tcgaagacca gttccaccgc
     5821 atgattgcca gtggcgtcgg catccctgtg ggcatgaaga acccgacatc cggtgacatt
     5881 tcggtcatgc tcaactccat cgaagcggcc cagcatacgc aggatttcct cttccgcggc
     5941 tgggaagtcc gcaccaaagg gaatccgctg gcccacgcca tcctgcgcgg ctatgtcgac
     6001 agcttcggca acagcatgcc taactaccat tatgaaaacc tccagcgcct gtacgaagcc
     6061 tatggcaagc gggacctgtc ctatccggcc gtcatcgtcg acgccaacca tgccaattcg
     6121 ggcaagaaat acctcgaaca gatacgcatc gccaaagacg tcatccacag ccgcaagcac
     6181 tcggccgaca tcaaacagct ggtcaagggc ttgatgatcg aaagctacat cgaagacggc
     6241 aatcagaaaa tcagcgaagg cacctatggc aaatccatca ccgacccctg cctgggctgg
     6301 gaaaaatccg aacgcctcat ctacgatatg gcagaattgc tgtaattagt ggctcgtggc
     6361 tagtggttcg tttttgcggt cttatagccg cactgtagga gcttctctat agtttgtagt
     6421 acggagtttg tagtttgtag aagagggcct taaattaaaa accaacccct agcccctaac
     6481 cactagcccc taaccactaa aaaaaaatcg ctgtcgtacg tgcgacagcg atttttttta
     6541 aaaagtatcc aacaatacat atcctttacc gcgcaggaac gtttccgttt ccatttcgcg
     6601 ggtcttgaag atggcgaccg gtgtgccggc ctggcggtta taagaaatat acaagtagtc
     6661 aatggaaata ttggcctggc ggaggctgtc gaggattttg tccagacagc ccggcgtatc
     6721 gcccatttcg acggcgacga ctttgtccat atgacattgg tagccggctt cctgcaattt
     6781 cgagacagcc agttccggat tggtgacgat gaggcggatg atgccgaatt ctgcgctgtc
     6841 attggccagc atggtgtaga tattgatatt ggcatcggct aagaccgacg tcatcttgcg
     6901 caaggcgccc ttcgtgtttt cagtgaatac agaaacttga ttgagcataa atcgaaatcc
     6961 cccttgatga taaaatattg tgacgttgtc cccattttac cataaaaaga caggcttgta
     7021 atggggatgg gatttgttaa acgtttcaga atgggcaagt catgatataa taaaagcatg
     7081 ggaactatgt tttttacgat acgaggtgag ggctatgcca gctcgattgg tcatcttctt
     7141 atttttactg ctcacaggca ctgccaaact ctatatgtgg ctcggcgtca cggacccctt
     7201 gaaagccgtc atcgcggcga ccctgacgac ctttgtcatc atctgtggtc tgcggcggag
     7261 cggccagaaa caggccgaac aaaaagaacg ggaaaagaca gcggcagctg aaaaagaaaa
     7321 aatccagtcg gcccaggaac ccgtaaagac gaaacatcga ctcgatgccg acgatgcggc
     7381 cttcctgtca gccgtcggca caggccttat cctgcacgaa ctccacgaac ggaacaaaga
     7441 acaagaacgg gtcgaacgcg attacgacgg gaattgggat aaccccgatg acgacagcca
     7501 gtatgacgat aaccagtacg acgacagcca gtatgacgat aaccagtacg acgatagcca
     7561 atatgatgac tgggagtgat gggaatttcg caggccgcag ttcgcagacg gcaggccgca
     7621 aaaaaataca cggccaacgg ccttgtgcgt aggggctgtc ccaagccttt ttggcccgtc
     7681 aggtgctgac cgtcgggagg cgcaagacgg ctgccaaaac ggacagctgt ggctgggcag
     7741 cccgttgtga gatttggcat acctattctt gtatgatggt aggtttttac gagccacgag
     7801 ccacgaacca ctattttttc acgagctgag ggttccttga ggcaatgtcg ttaatttgaa
     7861 tttatcgtcg cgcagcggca tttgaatccg aagtgaacag cggcctgcga actgcgggcc
     7921 gtcagaccct aaattcgacg aacgacctac gacgaacggc tctacgccgc ggcctgaggc
     7981 cttcatttcc tggagattcc gaaatatacg aaatcgccga agaagcgaat ccgtgacatg
     8041 acgaaaatgg agcgctggct ggcgtatttc gctaaccagc tcataccatc gtaggggtcg
     8101 tccgtgggcg acccgtcagc atgatagggc ttgtcttgtt catgcatgtg gattagaagg
     8161 agggaactga tcatgagcga tgaagccatc cataaagccg tagatgccgc ccggaccttt
     8221 cttcaggatg acgccgaacg cctggcttac atcaaccgcg aactcgccat cctagactat
     8281 aactcggacc atcgcgatgc ctttgaagaa ggggaagcta agggacgtgc agaaggaatt
     8341 gaaaaaggtc gtgcagaaga acggaaatcg tcagataacc gatgggaaaa attgatgggt
     8401 ctgttattgg aggaaaaacg gtatgatgaa gcgaaaaaag cagcttccag taaagagttc
     8461 cgtgaaacac ttttcaaaaa atatggctta gaataagaca gaatttagaa gtgaacgtac
     8521 tcccgcctct ttaggcggtg agatgaattg caggtttttc tgagggttcg atgccctcag
     8581 aaagacaatt gcttcataat ttgttttgca gtataatcag tagtaagaga gaaagaactg
     8641 attatactat gaaattagac agtaacaacc attcagtgtt cttgctctat taccatcttg
     8701 ttttggtggt gaagtaccgc cgcaaagtat tttctgaccc aatgagccaa tatgcaaaag
     8761 atatatttgt ccgcataggt tcatcatata atatcacttt ggaagaatgg aaccacgacc
     8821 aagaccatgt acatattatg tttcgtgccc atcctaatac agagatatcg aaatttg
//
LOCUS       DAWCYS010000047         8514 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_546_length_8514_cov_5.824920, whole genome shotgun sequence.
ACCESSION   DAWCYS010000047 DAWCYS010000000
VERSION     DAWCYS010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_546_length_8514_cov_5.824920"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2181 a         2314 c         2322 g         1697 t
ORIGIN      
        1 ctatacatat aagggaagag accacaagga atgaaagcca tacattcttt agacaggatc
       61 tccccacgaa aggaagggat gttttatggc aaccatcgtt agaggtaaaa atttagaaat
      121 tacaccggct ctgaaagatc acgttgaaaa acaggaaaag aaagttacga aatactttga
      181 taacgaagtc aatgtccagg ccctgctttc tgtagaaaaa gatcggaaaa tcgctgaaat
      241 cacgttgaaa gtcgacggtg tcgttctccg cggcgtcgaa gccaacgatg atatgtatgc
      301 tgctatcgat ttagtctatg ataaactggt ccgccagatt cataaataca aaacaaaaat
      361 cgcccgccgc atgaagaaag gcgctttcaa tgacgctctc gtcgaagggc cggctgttcc
      421 ggaagaagcc tttgaagtcg cccgcgtcaa gaaattcgcc gctcgtccga tggacgtcga
      481 agaagctatc ttgcagatga acctcgtagg ccacgacttc ttcgtcttcc tcaattccaa
      541 gaccgatacg atcaatgtcg tctataaacg gaaacacgga aaatacggcc tcatcgaacc
      601 ggaatactag gggttgtccg tggctccctt gataggggag ctgtcagcat agctgactga
      661 gaggttgaaa agatgaataa aaaagctgtc gcattaagca ggaatgcata atgcgacagc
      721 ttttttttgt attaagtttg caggagaggc aaataatggc ggtctgcgac tgcggcctgc
      781 gggctcgcca cgtgcgagcc cctactcttc ctcattgtgg aggacgctga ttttgggagt
      841 atggtggatg gcttgttcaa attcgtcctg gatttcctgg ctcggtccgc cgtcgagttt
      901 gctgacgatg tagatggcga tgagcgagaa gaggaagccc ggtacgattt catagaggcc
      961 gaaccaggcg aactgtttcc agatgaggac ggtcaggccg ccgacgatga tgccggccag
     1021 ggcgccgttc ttggtcatgt tcttccagta cagggacagg aggatggtcg ggccgaaggc
     1081 ggcaccgaag ccagcccagg cataggagac catggtcagg atgtagctgt tcgggttgag
     1141 ggccaggacg atggcgcagg cggcgacgac gaggaccgtc aggcggctga cgtagatcaa
     1201 ctctttatcg ctggcgttct tgcggacgaa ggtatgataa aagtccttgg aaatggccga
     1261 ggccgtgacc agcagctgcg acgaggccgt actcatgata gcagccagga cggccgataa
     1321 gatgaggccc gaagcaacag gcgggaacag ctgaccgcgc atgaccagga agactttttc
     1381 cgaatcggcg ccgactaagg gtgtcgggac gatgactttg ccgaccagac cgaccaggac
     1441 ggcagcggcc agggagatga cgacccaggt catggcgatg tgtttcgatt tcttcagttc
     1501 cgtcgggtcg ccgatggcca tgaagcggac caggatatgg ggctggccga agtagccgat
     1561 gccccaggcc agggcggaga tgatgccgat gatacccgtc gtcgtcgtgt ccggtgccat
     1621 ttccaggagg ttcggtgccg tcgtagccaa tcgggtcatg acggcgtcgc agccgccaaa
     1681 ggcgatcatg ccgaccaagg ggaccaggac gatggccagg aacatcatgc agccctggat
     1741 gaagtcggtc cagcagacgg ccatgaagcc gcccaggaag gtgtagatga cgacgacgcc
     1801 ggcactgagc agtagggccg agaagtagtc catgccgaaa accgattcaa agagcttgcc
     1861 gccggagacg aagcccgatg acgtgtatat aaggaaaaat ataaggatga aaatagccga
     1921 gatgatgcgg atgatatttt tttcatcgtg gaagcggttc ttcaaatagt ccggcatagt
     1981 caggctgttg ttggccactt cggtatagtt acgcaatctc tgggatacga actgccagtt
     2041 gaggtaggta ccgatggcga tgccaatggc aatccaggca gccgagatac ctgtagaata
     2101 agcgaagccc ggtacgccca tgagcatcca gccgctcatg tccgatgctt cggcactcat
     2161 agaagtgatc catggaccca ggctgcgccc gccgagaatg taatcgctca gccgctgctg
     2221 ggaccgggaa taatagacgc caatcgccag cattgccagt aagtagaggg caaaggctgt
     2281 caatatggac acatcatatt gcatcataat gataaaaccc ctttcacgtg gtggataaat
     2341 aaataataaa atcattttag catattcatg cggtgttgta tatgggaaaa tacctgttct
     2401 ttcccaaagg aatcccctta tattttctta aatccctttg aaacggttgc aaaataggcg
     2461 ccaatcacgg tataatgtat aattgatata atatcacaga gtggattgca aattgtgtaa
     2521 attggaggtc tcggataggt gtttaataaa ctgatacaaa gactgctcgg gaataacacg
     2581 gagcatgaat taaagaagat gtggcccatt gtccgccgca tcaatgatct ggaaccgaaa
     2641 ttggcaggcc tgagcgatgc gtcgctgcag gagaagacct tcgagttcaa gaatcgtctg
     2701 gccaacggtg aaacgttgga cgacattctg cccgaagcct ttgccgtcgt ccgcgaagct
     2761 tcgcgccgtg tgacagggat gcgccatttt gacgtccagc tcctgggggg tatcgtcctc
     2821 catcgcgggg atatcgccga aatgcgtacg ggtgaaggca agaccttggt cgcgacgctg
     2881 ccggtttacc tcaatgccct gacggggaag ggcgtccacg tcgtcaccgt caatgactac
     2941 ctggctactc gtgacagtga agacatgggc cgcatctata agttcctcgg tctgtccgtc
     3001 gggctcatcg tccacgacct cacgtatgac cagcgccgcc gggcatataa cgccgacgtc
     3061 acctatggga cgaacaacga attcgggttc gactacctgc gcgataacat ggtcatcagc
     3121 gaagaccaga tggtacagcg gccgctcaat tattgtatcg tcgacgaagt ggactcgatc
     3181 ctcatcgacg aagcgcggac gccgctcatc atttccggcc cgggtgaaaa atcgacggac
     3241 ctgtactaca cgctggcagg catcgtcaag accttcacca aagacgacta cacgatggac
     3301 gaaaaacaga agaccatcgc gccgaccgac tcgggtgttg ccaaagttga aaagatgttg
     3361 ggcatcagca acatgttcga caacgaccac ctcgatttga accacctcgt catccaggcc
     3421 ctgcgggcgc gcttcatgat gcaccgcgat aaggattacg tcgtcaagaa cggcgaaatc
     3481 gtcatcgtcg atgaattcac aggccgcctc atgttcggcc gccgttacag cgatggcctg
     3541 catcagtcca tcgaagccaa ggaaaacgtc aaggtccagg gcgaaagcaa gaccctggct
     3601 acgattacct tccagaacta cttccgtatg tacgacaagc tggccggcat gaccggtacg
     3661 gccaagacgg aagaagacga attcaacaag atctataaac tcgacgtcta cgtcatcccg
     3721 accaacaaac cggctatccg ccgcgatctg ccggacgtca tctacaagac gaagaacgcc
     3781 aagtaccgcg ccgtcgtccg ggaagtcaag aagcgccacg ctacgggcca gccgatcctg
     3841 gtcggcacga cgtccatcaa ccagtcggaa atcctcagcc agctcctgga taaggaaaat
     3901 atcgtccaca acgtgctcaa cgccaaatac cacgaaaaag aagcggaaat catcaagaac
     3961 gccggccaga agggcatggt caccattgcc accaacatgg ctggccgcgg cacggacatc
     4021 aaactcgggc caggcgtcgc tgaactgggc ggcctgatga tcatcggtac ggaacgccac
     4081 gaaagccgcc gtatcgacaa tcagctgcgc ggccgtgccg gccgtcaggg cgacccgggt
     4141 acgacgcagt tcttcctgtc tctggaagac gacctcatgc gtatcttcgg ctctgaaaac
     4201 atttccaagt tcatggataa attgggcatg gatgaagacg aaccgattac ggccaagatg
     4261 attacccggt ccattgaaaa ggcccagaag aaagtcgaaa gccacaactt tgaaatccgt
     4321 aaatacgtcc tggaatatga cgatgtcatg aaccagcagc gtgaagtcct ctacagccag
     4381 cgccgtcagg tcctcacagc cgattccctg cgcgatacta tcatgggcat ggtcgacgat
     4441 atcatccacg aaggcctgga caaatacgcc aatgaaaagc tctatcctga agaatgggat
     4501 ttcgccggcc tcctgagcca gatgcagcag tatttcgtgc ccaagaacgc tacgtctgtg
     4561 gaagaactgg aaaacctgag ccgcgtcgaa gtccaggata aattgaaaca gatcgccgtc
     4621 gacctctatg aagaacgcct caaggaaatc ggcgaacaga ccatgaagga actggaaaag
     4681 gccatcatgc tccgcatcgt cgacagcaag tggatggacc acctcgacgc tatggatgcc
     4741 ctcaaggaag gcatcaacct gcgggcttac ggccagaaga atccgctcgt cgaatacaaa
     4801 ttcgaagcct atgaaatgtt cgaggaaatg atcgaatcca tcaaacggac cgtcgtcacc
     4861 ttcctctatc atatccaggt cacgtacagc aagcccgtac cgcaggcgga agatcatctc
     4921 cagggcgctc acgaagtcca cgaagaaagc aaacccgtca gcgccgaaga catcaagaaa
     4981 gacgaagaac gggaacggca atgatggcgt aggggcgtcc acgtggggcg cccgcgcgaa
     5041 ttctgtgaac atcctattat agttattttt atttagaaaa agcaggtgat acttgtgttg
     5101 ttagatgaaa tgcgtaaacc gctggaagac ctcaaacata gaatacaaga aatcggggat
     5161 tcactttaac ttacctcgga aaaaagaacg tatcatggat ttagatatgc agatgagtga
     5221 cccgaccttc tgggatgacc cggacaaggc caagaagatt tcccaggaag cgacgctgct
     5281 caagacggaa gtcgaaggcc acgaagaact cgtccgcaaa gctgatgacc tcagcgacta
     5341 tttggaaatg gccatggaag acggcgacgc ctcctttgcc gaagacattg aaaagcagta
     5401 ccacgacctg ctcaaggaca tcgaaaagcg ggaagtacgg ctcctcttgt ccggcgaata
     5461 cgacaagtgc aacgccctca tcaccttcca cgccggtgcc ggcggtacgg aagctcagga
     5521 ctgggcacag atgctcatcc gcatgtacac ccgctgggct gaacgcaatg gctacaaact
     5581 gaccgtcctg gatatgcagc ccggtgatga agcgggcatc aagagcgctg ccttccgcgt
     5641 cgaaggcgaa tacgcctatg gctatttgaa atcggaaaaa ggcgtccacc gcctggtccg
     5701 catttcgcct ttcgatgccg cagcccggcg ccacacgtcc tttacggctg tcgatgtcat
     5761 gccggaactg ccggacgacg tcgttgtcga aatcaatatg gacgacgtcc gcgtcgatta
     5821 cttccgcgcc tccggtgccg gcggccagca cgtcaacaag acgagttccg ctgtccgcat
     5881 gactcacatg ccgacgggca tcgtcgtcca gtgccagaat gaacggtcac agctgcagaa
     5941 taaggaaatg tgcctgaagt acttgcgggc taagctcttt gaattggaac aggaaaagca
     6001 ggaaaaactg aaagccgaaa tcggcggcgt ccaccaggct atcgaatggg ggagccagat
     6061 ccgctcgtac gtgttccacc cgtacaacct ggtcaaggac caccggacca atgtcgaaac
     6121 gggcaatatc caggctgtca tggacggcga cctggacatc ttcatcgaag ggtatttgca
     6181 gcaggaaaaa gaaaagaagt tagcaaagca ggagctctaa agtaaatgaa aaaatggatg
     6241 attttctttg tcatcgtcgc agccctcttg gtcggcgcca attcctatgc gccccagctg
     6301 gccgaagccg gcctctgtca agccttgtcg gcgacactca atctgcacag cgatgatgtc
     6361 cgcgtccagg cgtcgccagg ggcgaaaatc ttcctcggcg acatcgacgc cattacggtt
     6421 catgccaata atttccaggc tggcgatctg accttcgccc acttcgactg caatctctac
     6481 ggcgtccatt tcaagccgct cctggccctg gccgatcagg aagtccgggt cacccgggcc
     6541 gagagcggtg agatgacggc atccatccgc agcgaagaac tggaaaagtt cctggtcaag
     6601 aaggtccagg gactgacgga cccgcaggtc tcctttgaag acgacgccgt ccgcgttcag
     6661 ggacgggtga agctgggcgg cctcgtgtcg gcccaggctg atgtccgcgg tcattttggc
     6721 atgaaagggt cgaagctcat gttcatcccg tcgtccgtga cggtagaagg catgggcatg
     6781 cagatcagca cgacgcagat ggccaatgct gagatttacg actttgccga attccccttg
     6841 ggaatccagc cggacagcgt caccatgcgc gacggcctcc tgaccatcca tggccaggtg
     6901 agcaactcat agataaggtg aagagaatga caaacatgaa taaacgcagt atgctggcga
     6961 tgatactggc cgtctttatc ggcctcgtat gtgcgctggg catgtgcgta caacgcaatg
     7021 cgatagaaaa tgagtcgacg acggtagaac aggctatgga ttatgatgcc atcgtcgtca
     7081 tggcacgtaa cgacggctac gacctcgaca cggcacttca gatgtgccgc gatgccggcg
     7141 ttacgagctt taccatttac gatactaccc tcaataagct gacccagcgc ggcgaactgt
     7201 cgctggtgac gaaactcggt gccgacctct attatcctca gttcggcatt accgacaaat
     7261 cctatgacta ctacctcatc ggcaagcccc agggccagac ggacctctat ttcgacgaag
     7321 tcgtatccga cctcaagacc cgcctgggtg acgataaagt caagatcatg tcccatggcc
     7381 cgtaccgtat tgtcggtatt caaggtgtca tgccgggctt aggcgatgtc aacctgggta
     7441 tcctcagcgc tgacgccaag actatcagcg accacggctt ccacgtcatc ctgcggccga
     7501 cgaattacag caacccgacg aaggaacagg tcgctcattt cttcgaccgc gtcgaccaga
     7561 tcaagaatgt atcgggcatc atgttcgtcg gcaaggaagt cctgggctat acgccggtca
     7621 acgccgaacg caagaccatg ctggactata cggcggacaa cctgaacgac agggacattc
     7681 ctttctatat gatcgaatcg gtcaaccagc tccagtataa tcagcaggac ggcatgtatg
     7741 acctggccgg cctggtccat taccgcacgg cccgcgtcta tgccatggcc aaggaagaac
     7801 tggaaaagat tacgcctgaa gaagcggcta tgcgctttta catcagcgac ctggaacgca
     7861 atgcccgggt caatctctat ccgctctata agaagcctct ccacggcatg aacctgaccc
     7921 agaccaactt gtcttacgtc aagatggttt cgcagaaact gacggaccgg ggctacaccc
     7981 tgggccgggc ttccatcatg ccggcctatt atccgaaccg cctgctcctg gccattacgg
     8041 cagcggcagc agcctgcggc ttcgtcttcg tcctcaacct gctcctgccc ttgacggacc
     8101 gcaagaacta catcctcatg ggcctgggca tcgtctgtgc cgtcatcggt gctatcgtcg
     8161 ccaaaggggc cttgttcctc caggtctggg ctatcggctg tgcgacagct gcgccgacag
     8221 cggccattct cctggccctg gaccactgga agaagatgaa gatcactaag aaactcggct
     8281 atggccgcgt cgtccgcgat ggcactatcg gcttgttctt tgccgtcgcc gtggccatga
     8341 tcggcggcct ctatatcgcc gccatgctgg gcaatatccg cttcttcatg gaattcgact
     8401 tctaccgcgg cgtcaagctg accttcatcc tgcccctgat cctggtggct atcggctatc
     8461 tgcgccgctt cccgctcatg ggacagacca tcgcctcgcc ggaagaccta aagg
//
LOCUS       DAWCYS010000048         8360 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_554_length_8360_cov_6.432029, whole genome shotgun sequence.
ACCESSION   DAWCYS010000048 DAWCYS010000000
VERSION     DAWCYS010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_8360_cov_6.432029"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2017 a         2115 c         2174 g         2054 t
ORIGIN      
        1 gtccccgctg ctgcggctgc catagaagtg aaaagccacg gcagcggcca gagccaggac
       61 gaggacgacg ataccggcga tgatggcctt ctgatagcgc acgtaaaaag gcttttcctg
      121 aaaaggcggc ggcgtcggcc ggcccgtcat gaccggtcgt tccatcggct gcggcggccg
      181 ctgcggctct tcgtcccgga ccggttcccg gtgcttatgg acaaaggttt ccgttcccgt
      241 cgcttcgtcg atgaccggtt cgtcatcatc cattttcggc agttcggcac cgcagaatgg
      301 gcagaatttc ccatcgacgc tcacccattt gccacattcc ggacaaatgc gtttcttcgg
      361 ctccccttcc ggcagctgtt cagcgccgca ggcctgacaa aagcggtcgt catcctgcaa
      421 gggcgccccg cattgttggc atttcttcat aaaatacaca tcctttactt agcgtaagta
      481 catgcttcta gtataagcca tttccctcag tcttgcaaaa aatcggccat gcgccggtag
      541 gtgtcgcaga tttcgtcgta gcatattttt tcaaagttga ggtacgaatg gaccatgttg
      601 ccgtaattat acgtttcggc ccgcacgccc ttttcagcca ggcggcgcac gtaatcgcgg
      661 ccttcgtcgc ggaaggggca gaactgcgtc gtcaagacca gcgtcgccgg catatctgcc
      721 gacaattcgc caaagagggg cgaggcatcc cggcggtcgt caccgggacg gaaacactgg
      781 ttgaaatacc aggtcatctt ggccggcgta aagcccgtct gggtattgca gttctcctgg
      841 accgacggga aggagtgggt catatccagg cagggataga ccaggatctg atgagtcagc
      901 ggaagggatg catcatgctg cagggcctgg ctcgtataag ctgccatgca gccgccggcg
      961 ctgtcaccgc acaaggtcag gtccgcctgg taggggatct gcaaggcatc gagcgcaggc
     1021 agggccttcc gggctacggc cagggcatct tcataggccg caggataagg attttccgga
     1081 gccaggcgat attccggcga aatgacgata tgctgcgttg cctgggcaat gcggcggcag
     1141 accgggtcat agacggcgac cgtgtcgatg gcaaaaccgc cgccatgata gtagatcagg
     1201 accggcaggg gccggctcgt atcgggaata tagatgcgga ccggcacgtc gtagacctca
     1261 cagccgtcca ataccgtatc gacgaccgat gccatcggca ctttcggcag ctcttgttcc
     1321 catgccgtat gcagcgacgc ttcgcgcatg gtaatcggcg tcatgacaaa atcctcactc
     1381 gtatgggcag cgataaaagc tgccagttcc gttttaattg cactcatcgt aaacccctct
     1441 ttccctcttc ttatatagtt ttaaattgta acaaaaaata tcccttttgg caccgtaggg
     1501 gtcgccacgc gggcgacctg ctggatactg ggcggcactc tccctaaacg ggaaaatcgc
     1561 tcatatcctg gagcgggctt gccacgtgca agcccctact acagaggtgc tgcttatata
     1621 gtttttaatt gtagcacaaa acagcacgca tgaataggat ggatacaaaa aaagaggctg
     1681 tcgcattaag acagcctctt tttttgagtt ttaagtttgt agtttttagt tttcagcaaa
     1741 tggtaaaaaa tttaaagcca cttctacaaa ctacaaactc cgtactacaa actataaata
     1801 aaaaggggct gcgcacgtga cctaagcctc atgcgacagt ccctctaaaa gtcctgtgtg
     1861 gcggagagcg tgggatttga acccacgata gagcgttcgc cctatacatg atttccaatc
     1921 atgctccttc agccactcgg acagctctcc atggcttaca cattgtttga ttatgcagtt
     1981 atttgatgcc ctcgggatcg acatccaatg tgtaaaaata tggtcggggc gacaggattt
     2041 gaacctgcga cctcttagtc ccgaaccaag cgcgctacca aactgcgcta cgccccgttg
     2101 acccgactca tacataatac catatccacg aaaaaagtca aggaaaaaat tcaatcaccc
     2161 catcaggcca gcttcttttc ccggatgaaa tcgatgagca tggccgacgt atcggccatg
     2221 aggtcgccgc acgatacggg accgccgttg gccggactct tgaggacgcg gcagcacgtc
     2281 gcatgaaagc ggtcgagcca gattttctgg aattcccgca caggctgatt gacttcggcc
     2341 ttcgtctttt cctccggcgt cgtgcggccg acgagacagc ccaggaccag acaggccccg
     2401 ttcaaagccc cgcaaagaca gcccgaaccg gccaccccgc cgccgaggac ggacaccatg
     2461 cgcagcgttt ccggcggcag cttcaaatcc agacaatcat tgcagacctt gaccaccgtt
     2521 tcagcacaat tattcccggc ctgctggtac tgtgccgcca attctcttac cttttccatc
     2581 ataagacctc cttgaaaaac agcatttgta gtacagagtt tgtagtttgc agaaaaatgg
     2641 ctttaaattt aaaatcattc actaaaaact ataagctgca aacttaatgc tcaaaaaggg
     2701 gatgccgcat atgcgacatc ccctttttac attctggcgg agagcgtggg atttgaaccc
     2761 acgatagagc gttcgcccta tacatgattt ccaatcatgc tccttcagcc actcggacag
     2821 ctctccatgg cttacacatt gtttgattat gcagttattt gatgccctcg ggatcgacat
     2881 ccaatgtgta aaaatatggt cggggcgaca ggatttgaac ctgcgacctc ttagtcccga
     2941 accaagcgcg ctaccaaact gcgctacgcc ccgttacctg acttgtatat aataccatac
     3001 tggaagaaaa agtcaagctt tttttctcgt atcaggcgac aactttcagg cgacaacttc
     3061 tttattttta tggatccgct tccaaatgaa tccgccggca atggtaacga ccagcaggat
     3121 gattttcagt tccgtcggat tattggccgc caggctgacg tattgagcca ggtaatggtc
     3181 tgtcgccatc atgccgcccg aagcccagcc gaggatggcg cccccgatgt agatgaggat
     3241 gggaaagcgg ttcatgactt tgacgacgat ggtgctgcca aagacgatga tcggcacgga
     3301 aatgagcatg ccgaacatga gcaggcccca atggttattg gtgacgccga cgatgcccag
     3361 gacgttatcg aggctcatga gggcgtcggc catgatgatg gtccgcacag ccgcccagag
     3421 cgagtccttg gcttcgacgt ggacgtcttc ttcctggtcg tcggcgccga tgagcttgat
     3481 gccgatgagg accagcagga tggctccgac ggtcttcaag aagggaatct ggaagagcca
     3541 ggacacgata aaggccatga tgaaacgcat acagatggcc ccggccgtac catataaaat
     3601 agcccgcttc tgcaaatgct taggcagatt cttggcggcc atgccgatga cgatggaatt
     3661 atcgccgcct aaggccaagt cgatgagcac gacctgcaaa atcatagtga tatcaaagac
     3721 aagcgtcgat tctaaccctt ccaaggtaag tcccccttat acgttaagac aaatttacat
     3781 ctcccccatt atatgataat tacggtggga tagcaagaaa aaggcgctgt cccatgaaga
     3841 cagcgccttt ttctgtaggg gcgtccacgt gggacgcccg ctcacacaaa tcaagtttac
     3901 aagtatctcg catatccccg gcgggctcgc cacgtgcgag cccctacaaa caccatatta
     3961 cgagcgacaa atggttctta tttcacgatg accgatacgc catcggggat gacggtgatt
     4021 ttcgggtcgg taatgccttt tttcgccagg acgtctttgg ccatagccag ggcgtcgtcg
     4081 atggaggcag cgtatttcat gtgcatgtct tcgaccatct gctgcggggc ttcagtgacc
     4141 atgatgatag tgaatttcag gagcatacgc atgaggatct gggcttccca ctggtcgaag
     4201 accgtttcgt tgcggccgcg ggccaggatt tcgtccatgg ctttctggat ggattcggca
     4261 ttcttcaggt tgttgtagaa atcttcgccg ccatgaccgt cagagcagga cgatacgtcg
     4321 atgatgacgc cgccttcttt gcaggtcgct tcggcagcgg tcatgccttt gacggactgg
     4381 taaatgttct ggtccagggg atagccgccg ttggtggtga tgacgatgtc agccgggacg
     4441 ggtttgacgc cagccagttt catttcaaag tcaacgccgg catagtgagc tttttcgcgg
     4501 tcgccggcaa aggcagcgat gactttcttg tccgcatcga tgacgacgtt gcagatgaag
     4561 gctaatttag ccgctttagc cgcatagagc atatcttcat ggatgggatt gccctggagg
     4621 atacccgtgc gggcgttggg gctgttgatg aattccgagc agtggttggc caggaccgtg
     4681 accttgctgg caataccagg caggacggat ttacgtccac ccgacatgcc ggcgaagaaa
     4741 tgcggttcga tgaagccttc ggaaatgagc aagtccgcat tagcggctac tttgttgata
     4801 cggcagtcac caccagaggg caaagtacca acgctgacca tgtcttcgtc gttgcggctg
     4861 acatggatga cgaatttttc atgttccgca atttctttgc catacttggc aattaattct
     4921 tccttagtcg tagcccggtg catgcccgta gcgatgagga tggtaatgtc gatatccggg
     4981 ttccccttgc gcagttctgc caggagctgg ggcatgatga tgtggctcgg taccgggcgg
     5041 gtatggtcac tggaaataat gacgcagttc ttcttcccct ttgccaattc gcttaatttc
     5101 gggctgccaa tcgggttggc cagggcatcg gcgacgagtt ccgcttccga tttcggcgcc
     5161 ttgtattcat gggcgtggga gacgaggacg ccggcgacct gttcttccgg cagttcgaat
     5221 ttgatttttt ctttgccaaa gggcaagcta aatgtatgca taactgcaca tcctttctta
     5281 gttcatggct atatatatgt ttgtgaatag cctgtagttg cataaacaat atgtttttgg
     5341 atgtaaaaaa gaagaaaggg caaagccttg cgccttgccc ttttccttta gtatgtaact
     5401 gtgaattatt cttcgacagg gacttcgtcg ttgatgctga caactttgcc cggattcatg
     5461 atgttcttcg gatccagtgc tttcttgaca gctttcatca tcttgagttc aaccgggtcg
     5521 gtgtattttt caagaagtgc ttcacgtttg tagccaatgc cgtgttcacc ggagagacgg
     5581 ccgccgagtt tgtagacgag agcgtagatt tcgccctgga tcttcggaag catttcttcc
     5641 cattcttcct gagtacggtc atctttcaag atatcgatat ggacgttgcc gtcgccgcag
     5701 tggccagcgc agtgcatagc aacattgtat ttttcgccga ggcgggaaat ctgctgtaca
     5761 gcacccggga tttccgtcat cggaacgacg atatcttcca tggagaagac taagctgcga
     5821 gcgcggtcag cttcgaggta tgctttacgg gatttccaaa taacagccgg gtcagcaacc
     5881 agggagtcga tagccttgtt gtcttcagcg agttcaacga ttttttcgca ctgatccatg
     5941 aggacgtctt cgttgtcgcc agcgatctgg atgataatgt agttaccatt ttcagatttc
     6001 ggcagttttt cgtcgaggaa tacttcgacc tgtttgatgg aagcgttctc catgaattct
     6061 acgcagaccg gagtgatacc agcgttcatg actttcggtg tcaaagcgat agcatcgtcg
     6121 aggttgtcga atacaaccag gaggtcctgc tgatatttcg gaagagcaac caatttgagg
     6181 taaactttgg tgatgatacc gagggtacct tcagaaccga tgaagaggtg catcagcatg
     6241 tagcctgtag cgttcttacg gaatttaccg ccgagagtta cgacttcgcc agtcggagta
     6301 acgacttcca tgcccatgac ctggtcacga gttacgccat atttaacagc gcggttgccg
     6361 ccagcgttgg ttgcaacgtt accgccgatg aagcaggagt ccccactgca ggggtcgccg
     6421 ccgtagaaga ggccttcctt ctggactgca tcctggagga cagacgtggt aacgccggct
     6481 tcggaaacac aaaccatgtt gtctttatcg atttcgagga ttttgttcat acgttcaatg
     6541 gagatgatga cgccgccgaa ggtagcaaca gcaccagctg ccaggcccgt accagctgca
     6601 cgaggaacca tgaccgtatc gttttcatag cagagcttaa cgactttgga aacttcttca
     6661 gtagtagccg gaagaacaac gacctgcggt tctttcatgt aatgggggtc cgttacttcg
     6721 tcatgggaat acggttcgag tttatctttg tccgtataaa catatttttc gccgacgatc
     6781 tgtttgaact gttcaatcaa ctccggggaa actttacctt ttgccataat acatacctcc
     6841 aaataattaa aattataagc ctaacactta ttgcttcatc tagtcatgac gaccagatct
     6901 aagaccgaat ataacgaaag tataaattca tgcatcattt ttatcttccg tttatctctt
     6961 acgggtatta ttatactcta cttcttcatg catgtaaatt acatttgcac attttacgca
     7021 tatcattttt cgattgattc atcgtgttct atgacaactt atcataagaa ctgtcgattt
     7081 ttaaataatc taaaacaaaa cgtaaaattt taaatagtat ttattttttc ttattttcta
     7141 tatatgcatc ttggtaatac agtattattc ccgcaaggaa atgtttcgca tatagaacat
     7201 ttcacatccg attatatcac attgtttatt atagaaaaag gtatgctaca tggatatgta
     7261 gcataccttt ttatgcatct ctgctatatg gcgttttcta ttcgcttagt tgactaagaa
     7321 gggaacgacg ctcggagcga tgatggtaat gatacctgca atgatgagca ggaacaggca
     7381 gtaaccaaca gtaccgcgca tgatttcgcc ttccttgcca tcagcaacgg caccgacagc
     7441 gccgacgcca agagcgatac tctgcggcga aagcatcttg ccgatacaac caccgaggga
     7501 gctggcacct gcaagccacg gctggctcat gcccaggctg gatgcaacgg atgtctgcaa
     7561 cggcccgaag aggacgttgg cgttcgtgca ggacccggta atgaatgcgc caagaccgcc
     7621 gacgaacgga gcaacgatag gatatgcatt gcctgttgca aatacgaggg catcagcgat
     7681 ttccttggtc atgccggagt aggtcataat cttagcgacg acgacgacgg cgatgatggt
     7741 caggtacgtg aagcgcaggc cgacgaagtt atctttgaag acctgagcga tcttaccgat
     7801 ggaagcaccc tgatagaggc cgccgaggat ggtggcgatg aaaatcatga cgccaggaac
     7861 agcgatccat gtgaaggtgt agggtttggc acccgggccg gcatagatag gaacagtcgt
     7921 tttaatcgaa gccagcggac cgttgatgaa cgggaacagt ttcgacgtac cgacgaggaa
     7981 tatgaagata aggatgaaga tcatagctgc tttgacgcct tcaccgaagg aaacagcttt
     8041 ctgatttgtc gtgtcaatat cgaacttgta ttccggattg cttgtcgtct tgcaagcgcg
     8101 ggaatagagg acgatgacag ccatgatgat gatggacgaa accatgaccg gcagttcagc
     8161 gccgatagtc atggaaatga acagttccgg aatagccagg gccaggccgg agagcagggt
     8221 tacgataccg acgcctttga tggctttgat accgccgccg atgcagatga cgatcaggaa
     8281 cggagcgatg aggagcggaa gaacggcttt atgctctact tgcgtagcca gcagcccggg
     8341 aaacacaacg aggcacgcag
//
LOCUS       DAWCYS010000049         8254 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_564_length_8254_cov_7.104525, whole genome shotgun sequence.
ACCESSION   DAWCYS010000049 DAWCYS010000000
VERSION     DAWCYS010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_564_length_8254_cov_7.104525"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1740 a         2236 c         2350 g         1928 t
ORIGIN      
        1 cattgcggtc agcgacctag tatgccgact tcatatcgtg agggaggatg agtgacgtcg
       61 catggatctg cttctgcata ctgacgacca ggcggttgat atccatacag cgcagcgggt
      121 ccaggccggc tgtcggttca tcgtagagga tgacctgcgg gtccagggca atggccctgg
      181 ccaggctgac gcgtttcttc atgccgcctg ataaatcgtt gggcatcatg tcctcgatgc
      241 cttccaggcc gacaaggtgg agtttctcct caacgatgcg ctggatcgtc gcttcatcgt
      301 ctttcgtatg ctggcgcagg ccaaaggcga cgttctcgcc gacagtcatg gaatcgaaga
      361 gggccgaata ctggaagacc atgcccatgt ggcggcgcac agccgtcagc tgttcctcat
      421 caaaggtagt aatgtcttca tcgtgaaaga agacctgccc tttcgtcggc ttctgcaggc
      481 cgatgatcag gcgcaggatg gtactcttgc ctgaaccgct ggcaccgagg atggccaggg
      541 tttcaccctg atggatatct aaattgatgt tgtctagtat gacccggtcg ccataggcga
      601 cggtcacatt gcgaagacgg atcatagcac tcctcaatac aatagaaccg ataagatata
      661 attgcagata aagatgaaaa taatcgacgt gacgacggac ttcgtcgtag cctggccgac
      721 gccttcggcc ccgctctggg catgcatgcc ttcattgcag gcgacgaggg cgatgatacc
      781 gccgaagaac atggccttga tgaggccgta gacgacgtcg ctgacttccg taaagagttc
      841 gatggaattc acataggtga aatgcgtgag cccggcgccc aatgtggcca cgccgtagcc
      901 gccggccgta ccgatgacat agccgtaaaa ggccaggata ggcaccatga cgacgcaggc
      961 tacaaagcgc ggtacgacga gataggcaat gggattgacg gccatacagc gcagggcatc
     1021 gatctgttcc gtgaccttca tggtcccgat ttcagccgtc atggctgcac cgacgcggcc
     1081 tgccatgacg acgccagtta aaatagggcc taattcacgg cccatggcga cggacatgac
     1141 gccgcccagc gtcgactggg cgccatagcg caagagaata tcgacgatct gcaaggtcat
     1201 gaccatgccg gcaaagagca gggtcaggct gacgataggc agggaattaa cgccgagatg
     1261 agccatctgg gcaagggtca ggcggcgttg tatctttggc agctgacgca gggtctggcc
     1321 aaagaggatg gcaatgacgc cgacttgttc aaagacacgg agcgtaaagc tgccgatgcc
     1381 ttccaaaatt tcctgcatga aagaccgtcc tttctttaag tatcatacta ctttactata
     1441 ccatcattaa aaatggatgt aaaacacctg tattcaattg tgcctaaatt gtgagaaaga
     1501 aaataaggaa aactaaaatt gcaatttgag ttttctacag ctaaaataat agcattttat
     1561 gcgaaaaatg gcaagggaaa aggcgctgtc atatgaagac agcgcctgtt tagtggctag
     1621 tggctcgtgg ctcgtggtta gtggctagtg gctagctgct gactgcgaca tgcggcctac
     1681 ggcctgcggg acgccctttg ggcgtcccct acgaacggct agtggaacat atccagatcg
     1741 acggtgcgga tcggttcagc cgatttctta tcctggcctt tatcgggttt cttgtcgctt
     1801 cccttgtcat ctttcttggt tgtatccttg ttgtcatcgc tgctcttatc ggctttgacc
     1861 aattcatcat cctgcgagga cccgtcggtt tcttcaccga tgacggcttt cgcttcgttg
     1921 atgttgtggt tcttggccaa ataagcgtcg cgtttttccg gcgtgacgac tttgatggtg
     1981 atctgaccgt cgcctttgac gatatacggt gtcggaatgg aagatttggc gtcgttcgga
     2041 ttttcatcgc cgatgttgtc cttgcccggt ttgatgacat ccttggcgct ggtccccggt
     2101 tctttgccgc ggatcttttc cttcttcact ttcgtgccgc tttcgttgat catgctgacg
     2161 ccctggctga ggatttcgat gaccaagtca ttgttggcat cttctttttc gatttccttc
     2221 ttcagttcat tgaaatcctt ataatggcgc aggcgctgga cgatttcgtc agtcaggttg
     2281 tagcgctgtt tttcgatgag gtacggcaga ggaatgacgc cgccgccgcg cacttccagg
     2341 accatatcgc cgagaggctg gttcttgggg acggtgaagc tcatggtctt cacttcatcg
     2401 acaccgcggt acgggtggag cttgaccttg aaatagaccg tatcgcccgg gctgacaacg
     2461 acaggcgctg ccgacgcatc gacgatggtc gccgacttgc aggcctgcgt gatatcgacg
     2521 gacatgttga tgtccaggat aggatagtcg atgaactcat tgtgtttcaa gatatccagt
     2581 acattgtaga gctcgtcgat actcttttca ttgatgttat cttcggaata atacatattg
     2641 tgccgggtaa tatctttatc acggccattg gaagagcgga tggtatacga caaggtcgcc
     2701 gtgccgccgc ccgtgcggtc gatggtctta ttgaccgtat tgtacaccgt cgtagccgcc
     2761 aggaccggcg tcagttcatt gtcttcgatg atcttgacgc gcttggtcat ggccgtgtcg
     2821 gtgtccgtat ccctggcctt gatgacgacg ggaattcccg gtgccagata gccatactgg
     2881 ccgccgatgc cggcgccgcg gtcctgagtg attttgccca cttcggcacc gatagagccc
     2941 agcttaaagg aactggacag gttattgacg actgtaaaga tgtaggcatt gtgcatgaaa
     3001 taattgatgc tgcccttttt caggaaggga tggccaaagg cgacgatctg gtcgttgtcg
     3061 acgtaagtga ccgtcccgat ggcacccatc ttcatatcgc cgttgacgaa ggctgcggcg
     3121 acgctgctgc ccggttccag gggcagggcc gtgctgtcgc tgctggccga agccgtatcg
     3181 acaggcatga agttatagcc cggcagtttg ctctgcatcc acgctgtcga cacgctgtcg
     3241 aagcccgacg tcatgagcgg cgtcgcaatg gggatgagcg atgattcgcg ggcgttgaaa
     3301 ggccggattt cttctctcgt cggcacattc cagagcttga tcatatcgtt gataggcgta
     3361 atcatgccga cgcgggaatt ggtaaaggac cagccgtaag cgacggcacc gagcagtttg
     3421 ccattgatat agaccgggct gccgctcatg ccctgagcga tgccacccgt ttcttcaatg
     3481 aggggcccgg aaaacttggc caggaccaag tcccctgacg ggccgctgtt cttcatgacc
     3541 cctaagattt cgacggggaa ggtatcaata gtcgtaccgt ggacgacggt cttggcatag
     3601 cccgtcatcc ccggctggac ttcactgaca ttcatgaact cctgtgcctg tgccgaaaga
     3661 gccaggatac aggaaagggc cgcggtcaga cagacccggc ccaacgtacg ccgcaaggcg
     3721 gagactttct tcatcatggt atatctcatt tcgtttccac cacgacgaca acggcgcctt
     3781 gctgcacttg tgatcccggc tgaacgagga cggtcttgac gacgccatcc ttgtcagaac
     3841 gggcagccgg catcggaccg gccagggaat tcaccgtaag caggacatct ccctctttga
     3901 cggctgcacc gacaggggct acgctgacga cgctgccgct caataccgtc gtgtacgata
     3961 cgtcagcagc tgcggccggc atagccatca gacctgctaa cccagctaat acggcgagtc
     4021 tgcgaaactt tttcatgacg gctccctcct tctcagctcc gcctgctgcg gagcactcta
     4081 taaatcatca aaactatctt ttaagcggtc catttcccgc aaagccttac cggctccgtt
     4141 ggcgacgcac aacaaaggtt catcgcagag ataggcggcc atgcctgtcg tctgggaaat
     4201 gaggcggtcg aaactatcca ggagcaggcc gccgccggta aggacaatgc catggtcggc
     4261 aatgtccgca gccagttcgg gcggtgtctt ttccaggatg gaaacgatcg tcttgaggac
     4321 ggtctgaatc ggttcgtcca gggcctggcg gatttcctgc gagtcgatat cgaccgacgt
     4381 cggcaggccc gtttccgtgc tgcggccgcg gacatcggct gtcatgcgcc ggccgtcgcc
     4441 catggccgtg ccgatgagta tcttcagctc ttctgctgtg tgctgaccga tggcgacgtg
     4501 cttgatcttt tccaaatagc ggataattgc ttcgtcaaaa gtgttgctgc caatgcgcag
     4561 ggattcgctg atgacgacgc cgctcaggct gaggacggcg acgtccgtcg tcccgccacc
     4621 catgtcgacg accatggaac cattagacgt ggcaatgtcc agaccggcgc cgatagcggc
     4681 agccagcggt tcttccatga ccgtcgtctt gcgggcaccg gcctgcatgg ccgcttcgat
     4741 gacggcccgc cgttcgacgt tggtgacgct cgacgggacg cagatgacga cgcggggttt
     4801 aaagatcagt gccttgccga tggccttgcg gatgaagtag cgcagcatgt attccgtcgc
     4861 gtcgtagtcg gcgatgacgc cgttgcgcag cggatggtag gcgtggatgt tacgcggcgt
     4921 ccgccccagc atggcctggg cttcgcggcc gatggcgacg actttatttt ccatcgtatc
     4981 gacggcgatg acggccggtt cattgacgac aatgccccgg ttcttgacga acacgatgat
     5041 attagccgtt cccaggtcga tagcgatatc gctgccgaac cagttggcca gaaagaacga
     5101 atgggactgt ttcggctgta ctcttttctt agttttcttc ttcctcaata ttgacgcgaa
     5161 caatgtctgc acctagcctt tgtaatttta atacgagatt atcataaccg cggtcgatgt
     5221 ggtataaata gccgacttcc gtttcccctt cggcgacgag gccagccagg actaaggcag
     5281 caccggcccg caggtccgtc gcattgacag aggccccttt gagcctgggg acgccttcga
     5341 cgatggcgct gcggccttcg atgcggatct tggcacccat gcgcttgaat tcgtcgacgt
     5401 gcatgaagcg gttttcaaag accgtttcgg tgacgacact cgtcccctgg cagatagtcg
     5461 tcaggaccat gaactgggcc tgcatgtctg tcgggaaacc gggataaggc agggtcttga
     5521 tatcggccgg gcgaagcggt tcatggccga tgacgcggat gccgtcgatt tcttcctgga
     5581 cttcggcgcc gacttctttc aatttggaaa tgaggggctt caagtgttcc gaaatggcat
     5641 tctctacgaa gacattgccc tgggtcatgg ccgcgccgac catgaaagta cccgcttcga
     5701 tgcggtcggg gatgacggcg tgattggcgc catgcagttc cttgacgcct tcgatgcgga
     5761 tgacgttggt gccggcaccg cggatgttgg cgcccatact gttgaggtac gttgccaaat
     5821 cgacgatttc cggttcttct gcggcatttt ccaggaccgt cttaccgtcg gccatggatg
     5881 cagccatgag gatattttcc gtagcgccga cgctggggaa atcgagatag atctgagccc
     5941 ctttgaggcc attcggtgcc gtcgcttcga tgtctccgtt ttccaggttg atgacggctc
     6001 ccatggcttc aaaagccttg aggtgcaggt cgataggacg ggcaccgatg gagcagccgc
     6061 cgggcaggga aatctgggcc ctccccttgc gggccagtaa aggtcccata accaaaaaag
     6121 atgcacgcat acggcgcact aaatcatacg gagccgttgt cgatgtcagc tcatgggcat
     6181 cgatgaccag tgtatctctt tcaggatgtt ctatatgaac gccgagggac gcgatgacgt
     6241 cacagacggt atgtacgtcg gctaatttcg ggatttctgt caaagtactc tgcgtcgttc
     6301 ccagcataga tgcgacaatg ataggcagca cggcattttt agccccgctg acgcgaaccg
     6361 taccctgtaa gggctttcca ccatgaataa caagtttctc caattctggt gcctcctaat
     6421 actattcgag tagtttattc taaatggcca aaaggcaatg gcgcctaagg tgccattatg
     6481 agcatataat tactatcaag tcattgtacc ataaaaggga agaaaaaaaa agggcttgtc
     6541 gccgtgcgat aagccctttt tacgttctcc ccaatccgga aaatcactca tattccggag
     6601 cgggcttgcc acgtgcaagc ccctacagac gaaattactc ggaaattttt cttcaaccta
     6661 caagcgggac gctaactgct gacgactgac tgctgactgc taacagctga cggctgacag
     6721 cggcctgcga actgcgaact gcggcctgcg aactacggct tttacaaatg aatactccat
     6781 ttggccttct tgtcgaagcg gtggaacgtg tcgatgaagc ggatcgtgcc gctcttgtag
     6841 cgcatgacga cggacatggt gcgggcgccg ccgccaaagt actggacgcc tcgcatcatg
     6901 ggcgtatcgg taatggccgt cgccgagaag atgcagtcgt cgcctttgac caggtcgtcg
     6961 agggtcatga cgcggttgat gtcatcaatg cccatttccc agcagcgctt ccgttcttcg
     7021 tcggtatagg gaatgaggcg ggcctgcata tcgccgccca ggcatttcag ggccgctgca
     7081 gccaggacgc cttccggagc gccgccgatg cccatgagga cgtggacgcc ggacccttcg
     7141 ataccggcgt cgacagccgg cgagacgtca ccgtccgtga tgagcttgat gcgggcgccg
     7201 gcactgcggc attcgtcgat gatttcctga tggcgcggcc ggtcgaggat gacgacggtc
     7261 agatcgtcga tgcctcgttc caggccttcg gcgatgcggc gcaggttttc ctgaacgggc
     7321 tgggtcaggt cgatgcggcc tttggcccgc ggtccgacac agagcttttc catgtacatg
     7381 tccggagcgt ggagcaagtt ccccttgcgg gcgatggcca tgacggcaat ggacccatcc
     7441 tggcctttcg ccgtcaggtt cgtcccttcc agcgggtcga cggcgatatc gacttccgtg
     7501 ccgccggcgc cgactttttc accgatatag agcatcggcg cttcgtccat ttcgccttcg
     7561 ccgatgacga cagtcccatc aataggaacc gtcgataaaa tcgaatgcat cccatcgaca
     7621 gccaactggt cagcgcccat cttgtcgccc ttccccatga ggcggccagc cttgacggca
     7681 gccgtttcgg taacgcggac aaattccaag gtcaatacgc gatccatagt gaacctccta
     7741 tataaaaata attgaaatac atatatagta ttacttctag acagagtaaa aaaactcctg
     7801 cctcggggca ggagtttttt cgttcatcgt ttatcgcagg cgaacgcctt tttcgagttt
     7861 gaagtttgca gtttttagtt tgcagcaaat ggtaaaaaat ttaaagccag cttctacaaa
     7921 ctctgtacta caaactataa agtaaaaggg accgtgtatg atggcaaaat ttttcgtgcg
     7981 acagccccct acgaacgcga ccggcggcct gcgaatgcga actgcgggac gccctttggg
     8041 cgtcccctac tactgtttcg tgatcacgtc gcaatgcatg tacgggcgga ggacttccgg
     8101 tacgatgacg gaaccgtcag cctgctggta gttttccagg atagcggcga cggtacggcc
     8161 gacagccagg ccggagccat tcagggtatg gacgaattcc gggttcgatt tcggcgtgcg
     8221 gcggaagcgg atattggccc ggcgtgcctg gaaa
//
LOCUS       DAWCYS010000050         8251 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_565_length_8251_cov_6.155320, whole genome shotgun sequence.
ACCESSION   DAWCYS010000050 DAWCYS010000000
VERSION     DAWCYS010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_565_length_8251_cov_6.155320"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6191..6200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1661 a         2284 c         2361 g         1935 t
ORIGIN      
        1 ctcgcttttg ctgactttga cgcacgctgc cggttccagc tcgtatcggg tgatagacgg
       61 cccctggctg gcattgagta tcttggcatc gatgttgaag ctctgcagcg tttcctggag
      121 gatgcgggca ttttcccgga cttcatcgct caggccgatg atatgctgcg gccccggttt
      181 gagcatgctc accggcggca ggcggtacgt cttttcgcca gccgctttgg gaacggcact
      241 gctcccttct tcgctggttt cgacagcgac gctgctcttt tcgacttcaa tgggcttgac
      301 gtcgcccata tcatggccaa ggacatccat ttctccggcc agtgaagccg ccggcgctgc
      361 cgggccgggc tgtacgtcat gggccgtcgg cactgtatcg ccgacgacgg tttcatgtat
      421 ctgactgtaa ggccgggcgt cttccggcat ttcctccggc gtttccggaa cttcttcata
      481 cgaatcgtcg ctggcccaat cgtaaggcac gtcttcttcg atagggctgg catcgaagcg
      541 gacttcttcg acgccgttgc ccaagtcgca ggcatcggca atggttgagg ccgggcgttc
      601 catatcagcg ggataatcat cgggattccc acaagcggga cgccctaacg ggtcgtcccc
      661 tacaaccgcc tgcaaatcga agatgcggcg gcgttcatgc cactgtttca gggactggga
      721 tgcgtcctga aggccttcga cggccttacc cgacactttg gccgcttcct ggccggcctt
      781 gtcggctacg acagccacag gcttagaaat agaccaattc ttccagacca ggaaggtgac
      841 gatgcaggca ccggtaatag ccatgacagc tccaaaaaat ccaagaaacc gccgcaggac
      901 caaggccatg acggccccga cgattccccc gccgacagat aagctgtgcg gcaggaattc
      961 cgtcccttcc gggatgacca gcaaatggac cagccccagg aggaggatat agatccacgt
     1021 cccgacggcc cacgatttcg acactgggca ggccttgcgg atgacgatgt atttcaggcc
     1081 caggacgatg agggacaggc tgacgacgat ccggcccagg ccgaagatat acgataagat
     1141 atcgtcgacg ctgtggccga tgcggcctgt atcgacgccg atgaagccgg ccgtcgcaaa
     1201 gacgcctgcg aggatacaac aaatacctat gatttcatat ttcaaaagtg gcgacgtccc
     1261 tgccttcctg gcagtcttcg ccttctgacg cgtgccagtc gtccgcttgg tgcggctggt
     1321 tttacgggtc tttccttttg tcaaaacagt tcaccttcta ctaatagatt acttgccgcc
     1381 acggcgctta tgccagccag ctgccatgct cccttcctgt ccatagggcc gttcccggta
     1441 ataggacgct cctttcaaag catcgggcag atactgctgg gccacccagc ccccatcgaa
     1501 atcgtgaggg tactggtagg tcaggccatg gcccagagcg gcggcgccgc tgtaatggct
     1561 gtcgcgtaga taatcgggca catcgccaca gtccttatgg cggacgtcgg ccagagccgc
     1621 atcgatagcc atgtaggcgc tgttgctctt cggcgcacag gccacgtaga tgacggcttc
     1681 ggacaggatg atgcgccctt ccggccagcc gacgaactgg acggcctggg cagcggcatt
     1741 ggcgatgacc agagcctggg gatcggccag tcctacgtct tcagccgcac agatgaccag
     1801 gcggcgggcg atgaaggacg gctgttctcc ggcgtcgatc atacgggcca tatagtgcag
     1861 ggccgcatcg gggtcggacc cacgcatact cttgatgaaa gccgataccg tatcgtaatg
     1921 ggcatcgcct ttcttgtcat acgtatagac cttatgaccg gctacttttt cgatagcgtc
     1981 gtgtgtgata tggccgccag ccgggacgac catgcagacc tgttccagca ggttgaggcc
     2041 gacgcgggca tcgcggtcgg ccagccgcgc cagggttccc agcatctcat cgctggccgt
     2101 gatgcccagc gagcccaggc ctttttcttt atccgtcaga gcccgccgca ggacggccat
     2161 gacggcctgt tccggcaggg gcttcagctg gatgacgcgc aggcgcgaca ataaggggcg
     2221 gttgacttca aaataaggat tttccgtcgt cgccccgatg aggatgaccg tcccgtcttc
     2281 gacatacggc aacaggacgt cttgctggct tttattgaag cggtggattt cgtcgatgaa
     2341 gaggatcgtc cgcttttgat ataaatggat ggcatcctgc gccgccgcga tgactttgcg
     2401 cagttccgcc gtcccggccg tgacggcgtt gagggttacg aaacggctgt ccgtctcatt
     2461 ggcaatgaca tgggccagcg tcgtcttgcc gacgccagag ggaccgtaaa acaaaaggga
     2521 cgggatcgtg tccttctcga tgagggtccg cagaaaactg cccgggccga tgaggtcgtc
     2581 ctgtccatag atgtcatcca aagattccgg ccgcatgcgg acggccagcg gcgcatagtc
     2641 ccggccgctc cgttcatcag ccatagaaaa taaatcgtct ccgctcattt gtttgctctc
     2701 cagactaaat agaacgccgc caagtctcct cagcggcgga cgaaataact aaattctcga
     2761 ataagaaaag agccccacca tgccgtttat aaaccgagtt ttgagcctgc ctaagcaggt
     2821 gggtgttctc ctataagggt cgacagtcca gtgaaggcat tgcctaacga tatagagccc
     2881 gaactcccaa tgtaaaaagt gttggttcaa aacatcgaga ccatttacgt acacagtagg
     2941 gtttcttctt gtatctagta taataaaata tagaagaaaa atcaagcctt gacaaatgaa
     3001 aaaggcgctg tcgcatgaag acagcgcctt ttaagtggtt aggggctagg ggttagtggc
     3061 tagtaggggc gccacgtggg gcgcccgttt ccgtatatgt gagaatcccg caaagacgcg
     3121 gcgggccgcc ctttgggacg gcccctacga acggctaact gcgacctgcg gtctgcggga
     3181 cgcctttgga cgttccctac gagccacgaa ccacgattat tttacgactt tctttacggc
     3241 ggctacgacg ttttctgtcg tgaagccaaa tttcttgaac aagacgccgg ccggagcgga
     3301 ggcaccgaag gatttcatgg aaaccgtcgt cccgtcgagg ccgacgtatt taccccagcc
     3361 gaagtccgaa gcggcttcga cagcgacgcg ggcgcggaca gctttcggga gtacctgttc
     3421 tttataggct tcgtcctgct gttcaaagag ttccatgcaa ggcatgctga cgacgcggac
     3481 gtcgacacct tctttagcca gttcgtcttt agcgctgaca gccagttcga cttccgaacc
     3541 gctggccatg atgatggcat cgggaacggc cttgttggca tcggcgatga cgtaagcgcc
     3601 tttcagggct tctttgctgc tgccggcgag ctgcggcagg ttctggcggg tcaggacgag
     3661 ggccgtcggc gtcttctggc tggtcgcagc caggtaccag ccggcagccg tttccgtagc
     3721 gtcagccgga cggaagacgt tgacgttagg aagggagcgg agcatggcca gctgttcaat
     3781 cggttcgtga gtcgggccgt cttcgccgac accgatgctg tcgtgcgtga ggacgtacgt
     3841 cgtcggcagg ctcatgaggg cagccaggcg gatcatgggt ttcatgtaat cgctgaagac
     3901 gaagaacgtg ccggcatacg gacggatacc gccgtgaagg gccaggccat tggtgatggc
     3961 gcccatagcc agttcacgga cgccgaagtg gacgttgcgg ccgctgtaat cggtcttgct
     4021 gaagaagcct tcgcctttca tttccgtctt gttcgacgga gccaggtcgg cgctgccgcc
     4081 aaagagctga ggcatgatgt ctttcaggcg gttgatcatc gtgccggaca agctgcgcgt
     4141 agcctgcggg ttatcttcat ccgccccgac atcggcggca gtcaggaggg ccggtgcctc
     4201 gagagcgctg tggaaagcat cccatttagc tttcatatcc gggaatttgg cagcgtagtc
     4261 ggcaaagagt ttctgccaat ccgcttcagc tgcgccggct ttctggctca attcagcata
     4321 atgagcatag acttcgtccg ggatgtagaa ggtttcctgc ggtttcggcc agcccaggtt
     4381 ttctttcatg gcgacgatgt tttcttcacc cagcggcgag ccatgagcgg cagccgagcc
     4441 ctgtttggcc gggcagccat agccgatctg ggtcttgatg gtaatgaacg acggatgttc
     4501 cttgtcggct ttggcttctt cgatggcttt gccgatggca tcgaggtccg tgccgtcttc
     4561 gacggtaatc gtctggaaac cgaaggcagc catgcgggcc tggacatttt ccgtaaaggc
     4621 gatgtccgta ttgccttcga tagagatgtt gttgctatcg tagaggacga tgagtttgga
     4681 aaggccgagt gtccctgcca gggagaaggc ttccgacgag atgccttcca tcaggcagcc
     4741 gtcgccgcag agggcgaagg tgtagtggtc gacgacggga taacccggtt tattgaaaat
     4801 agctgccaag tgagcttctg ccatcgccat gccgacagcc atgcccaggc cggcgccgag
     4861 ggggcctgtc gtcgcttcga cgcccgtcgt gtggccgtat tccggatgac ccggtgtcaa
     4921 ggagccatct tgacggaact gtttaatgtc gtccatggtc aggccgtagc cgtagaaatg
     4981 gagcagcgaa tacaacagca tcgagccgtg gccagccgag aggataaagc ggtcgcggtt
     5041 cgcccatttc gggtcctttg cgttatggtt catgtgattt gcccagagtt cgtatgcaat
     5101 gggggctgcg ccgaggggca ggccgggatg gccggagttg gccttctgga tggcgtcagc
     5161 cgacaataca cgaatggcat ttacacaagt cgtatcaatg gtgctcatta gtcaaatcgc
     5221 tcctttttat gtgatcgaaa ataaatagac attcacttta ttatgtaaaa aaaggacccc
     5281 ttctgtcaat ggcaaaaggg gattttggga gaaatttata tatgctataa taaagaagtt
     5341 tcttaatttt ccgagaaagg atagactcat gacgaaaaaa tactttttct ttgatatcga
     5401 cgggaccctg ggcctgggga tctccagcat catcccggca gacacgctgt actgcctgcg
     5461 ccgcctcgtc cagaaaggcc atttcatcgc catcgcatcg ggccgcctgc agcacgatac
     5521 acagaccttt gccgaccgct acggcatacc ggccgtcgtc gccgatggcg gcaacagcct
     5581 gtccctggac gggaaaatcc tgaaaatgac cggcctgccc ctggagaact gcaaagccct
     5641 gctgcgcgac ctgacggacc gccactatcc ctgggccgtc gtcacggaca ataccatcaa
     5701 ccgctataca ccgtatgctg atttccccca cgacgacccg cgcaattaca tggaaaccgt
     5761 catccgcccg gtccccatcg aaaatctgac ggctgtctat aaaatcatgt acgcccggcc
     5821 ccagcgcctc gaagacgaac cgccgacata cggcctgccc cacctggaat acatcgacca
     5881 cacgtacctc atcgaaccga ccgacaagag ccgcggcatc ctgcgtatgc tggacctcgt
     5941 cggcggccgc ccggaagatg ctgtcgtctt cggcgacggc atgaacgaca tttccatgtt
     6001 ccgcaagccc ttcttctcca tcgccatggg caacggccgg gatgagctga aagcccatgc
     6061 cgactacgtc acgggcgaca acgataaggg cggcatcctg gaagcctgca aaaaattcgg
     6121 ctggctctaa gggatagtcc gtagtttgta gtctgtagtt tgcagtttct agttgtggcc
     6181 tgcgcctttt nnnnnnnnnn taacagggct tcccgcaggc ggtcttcgat ctggaccagt
     6241 tcggcttcgg cggcctgccg cttctgccgt ccttcctgct ggatgcgcag cgtatcttcg
     6301 atggtgctga tcaaatcttc attgacctgc ttgaccgttt cgatatcgac gatggcccgt
     6361 tcattttcga cggccgtatc gtgggaattc tgcttgagca tggcggcgtt gcgcttgagc
     6421 agttcattgg tcgtattaga cacagcctgc tgcatttcca gggcttcttt ctgccgcccc
     6481 agccccaggg ccaggaccat ctggttcttc cacaagggga tggtgttgta aatggcactc
     6541 tggatgcggt cgacgaggat cttgtcgttg ttctggatga tgcggatctg cggtgccgtc
     6601 tggatggcaa tggtcttgct gattttcagg tcgtggacct tcttttcaaa gcggctgacg
     6661 gaattcttga aatcctggac gacctggacg gccatgggat tatctgaaac ctgagcctgg
     6721 gcttccagtt tgggaatcgt cgtcaagttc agttcctgta tcttttcttc gccagcctgg
     6781 atgtagagct ggagctgttt gaagtattcc aggttcttat cgtagaggcc gtcgaacatg
     6841 gcaatgtcct tcatgagggt cattttctgg ctgtccaggc cggcctgtat cttgtcgatc
     6901 tgggcggcca gcgtcgtata ggactgggtc agggcttctt ttttcttgaa cagcgagccg
     6961 acgacgggca ggctggccag gaagccctgg tcctcgtcgt tttcgtcttc aaaggacttg
     7021 acccgcttga ccaggtcgtt gagcaggtca ccggctgcgg ccgggtccgt actcttgacc
     7081 gtcgacaaga cgctgtccga aaaggtggcg atatttttct gggcggcgct gccgtactgg
     7141 acgatggcgt tggaattgac caggtcgatg gcatccttga tggtgtcgac ctgctgccgt
     7201 tcttccggcg tcaggctttc gacctgggca ttgaaatgag ctacttcgtg cttttcatcg
     7261 acggctggca gggctttctg gctgtcggcc tgctttttct ctaaaaggtc attcaaattg
     7321 atatcggcca tagtatctac ctactttctt tttggattca agaactggtc ggctaaatcg
     7381 atggtcgtcc cgcccaggtc atcactgcgc tgggcctgcg gttccacatt gggcggttcc
     7441 gtcgggatgc cgtcagcggc catgttctgc ttcatgacct tcatttcggc gtcgaggtcc
     7501 atgagctggg cattgaggac cttggagaac tggtcttcat aggcttcgtc gaacttatcc
     7561 aggagctgct tgatctccca tttcgactgc tgcacttcct gcgcttcaag gcccgtcttt
     7621 tccagctcct ggtacttgcg gaccaggagc agggcccggt cctggtaata atcgacgaag
     7681 cgccgggcca ggctcatccg ttccggatgc tgctgcaagt aggtcaggaa attcttggca
     7741 atgccctgca tcttgcgcag ctgtttccgc agttcgtcgt catcgacgtg cttcatttct
     7801 ttttcaatgg cgttgaaatc atcgacggct ttgctgaaga gctcgtcgtt cagcttgtcc
     7861 acttcttcgt tgccgctgga aaattccagt tccggcttga acttcttctt attgcggcgg
     7921 tgaatccagt agaggatgaa gacgccgaag acgagactga acgtcccgga cagatgaccc
     7981 gtagcgaacc agttataact gggaacggca aaacagatga tagcgatgag atataagaaa
     8041 atattcacga tcgtccctcc taagtgaaga atgtctttcc ttaatcatag catttttcgc
     8101 cccatttgtc gtggattgta aggcaaaaaa gaggctgtcc ctgaagacag cctctttttg
     8161 agtattaagt ttgcagtttg tagttttttg tgaatggttt taaatttaga gccattttct
     8221 acaaactgca atctccgcac tacaacctac g
//
LOCUS       DAWCYS010000051         8046 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_579_length_8046_cov_11.289826, whole genome shotgun sequence.
ACCESSION   DAWCYS010000051 DAWCYS010000000
VERSION     DAWCYS010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_8046_cov_11.289826"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1782 a         2129 c         2312 g         1823 t
ORIGIN      
        1 aaggaaggct gcaacaaaat gagcaaaaaa actcattttg ttacagcccc tttttattcg
       61 ctcttagtgg aacggagaaa tagatttttc aggacctcgt gggggtcctt tttttctttt
      121 atgatggcgt ggacggctgc cgtaatgggc agttctacgc tgtatttttc agacaggtcg
      181 aggagtgcct ctgtcgtgct gacgccttcg gccagttttt tgaagggctt tccttcgatg
      241 aagtcctggc caaagcggcg gttattgctg tgcagggaaa agagggtcgc ttcgtaatcg
      301 ccgaggtggc tcaggccgta gacggtcatg gcctgtccgc ccatggcggc gacaaggacc
      361 gatagttcgt gggcgccgcg ggccatgagg gctcctttca ggctggtcag atgagcgccg
      421 tcgagcatac cggcagcgat gcccatgacg ttcttggcgg ccgcgccgat ttcgatgccc
      481 aacaggtcct ggccgtagta gaaacggatg aggggactcg tgaaggtatc gactaagaag
      541 tgggtcaggt cttcgtcagc cgaggcgatg accatgcagt tcggcatacc gctgatgaaa
      601 tcctggacgt ggccgggccc gacccagacg gccgtgcgta cggacggtcc cagttcttct
      661 gccatgactg tcgtcaatcg cttgccgctt ccttcttcca gacccttcat ggccaggacg
      721 aagggcaggg acggggcgaa gccggcttct ttcagttgat gcgtgaacct gcgcagctgc
      781 tgggcgctga tggagatgat gcagatatcg gcggctgcca gggctgccgt caggtccgaa
      841 gtcaggctca cagaatcggg caggcgcagg tattcattct ggcgggtgtc cataagttcc
      901 tgcaagtgct gagaaccggg acggccccat aaggtgacgt catggccgat gtggtcggcg
      961 taccaggcgt ggaaactgcc ccagcggccg cagcctaaga cggtgatctt catggaatgc
     1021 ctccttttat cttacagctt ggcaatgacg gcgtcacggt attcttccgt cgtggcgctg
     1081 ccgcctaggt ccggtgtgag gtgctgcttt tcagccagga cttcatcgac ggctttgcgg
     1141 atcttggcgg caatggccgt ttcgccgatg tattccagga gcatgcaggc cgaccacaag
     1201 agggctgtcg gattggcgat gtgtttgccg gcgatatccg gggcactgcc gtggacggct
     1261 tcgaacatgg catagtcgtc gccgacattc atggaaggca gcaggcccag gccgccgatg
     1321 aggccgctgg tcaggtcgga caggatgtcg ccgtagaggt tgggcgtgac gatgatgtcg
     1381 aactgttccg gccgcatgac catctgcatg cacgtgttgt cgacgatttt gtcgtcgctt
     1441 tcgatctgcg ggtagtcctt ggcgatggtg tggaagatgt cgaggaacaa gccgtcgctc
     1501 atcttgagga tgttggcttt atggacgcag gtgacttttt tgcggccatg ggctacggcg
     1561 tagtcgaaag cggcccggat gatgcgttcg ctggccttgc gggtgatgag cttgatggcg
     1621 tggaccgtat cttcattcat cttgtattcg acgccgatgt acaaatcttc tgtgttttcc
     1681 cggaaagtaa cgatatcgac gttggggaaa ggcgtcttga cggccgtatt cgatttggcc
     1741 ggacggatat tggcatagag gtcatatttg ttgcgcaacg tgacgttgag ggaccggaag
     1801 cccttgccga taggcgtagt gatcggcgat ttcagcagga gatgggtctt ttcaaaagaa
     1861 gcgaatgcgt cgtccgggat gagggcgccg tggcgttcat attcggcagc gccgacgttg
     1921 aaaatttcat aggaaagggg cgctttggca gcgtcgagaa tctgcaggac agcgtcggta
     1981 atttccgggc cgatgccatc gcctttgaat acagtgattg tcttcatgat ctatctgtcc
     2041 tttctgggaa tatagtccat caaatcgccg acatacttgg ctgccggacg gacgatgcgg
     2101 ttatcgtaca gtttgtgttc gatgttgtgg gccagccagc cgaccatacg gctgatgacg
     2161 aagagcggtg tgtagaggtc gcgcgggatg cccagcatgt tgtaggcgaa gccgctgtaa
     2221 taatcgacgt tgctgcacat gttgacaccc ttttcttctt tcaaggtctt catggccagc
     2281 ttttcaaagc ggttgtagaa gcggaagacg tcggtcatgc ccttttcctt ggccaggatg
     2341 ccgcagtagt ggcgcatgat ttcagcgcgc ggatccgaca gggtgtagac ggcgtggccg
     2401 aaaccataga ccaggccgtc gtgacagcct ttgaatttct tgttgagaat ctggtggatg
     2461 acgtcgacga tctgttcatc cgtagccttg atgccgatgg cgtcgatgac ctgttccatc
     2521 atcagggata cgcggatatt ggcaccgccg tggcgcgggc ccttgagggc gccgacggag
     2581 ctggccatgc tggaatagat gtccgtatcc gtcgacgaga cgacgatatt ggtgaacgtc
     2641 gagttcgtgc cgccgccgtg gtcggcatgg aggaataaga gcaggtccag gagctgggct
     2701 tccttgggcg tgaatttgct gtccggccgc agcatggaca ggatattctc tgccgtcgaa
     2761 tagtccttgc ggggatagtg gatgaccagg ctcttgcggt tataataatg gaccttggac
     2821 atataggcat agacggcgat ggacggcagc ttgctcatga tattcaggcc cttgcgcagg
     2881 gtctggtaga cgtcggtatt gtccggatcg gggtcgtaat tatagagcgt cagcagggcg
     2941 tgctgcaatt tgttcatcag gttcttgccg ggcagacgca ggaggttcat ttccagaaaa
     3001 tcatcgggca gatcgtagcc gttttgtaag accttgcaga aggcattcag ttcatcttct
     3061 gtcggcaagt agccgaagag gaggagaaag ctggcttctt catataagta accttgataa
     3121 ttttcgactt tggccagctc gcgaatgtcg atgccgcgat agtacaagac gccgtcgcaa
     3181 tcgacttttt ggcctttttc gtcacgctgg tagccgacga catcggcaat gcgggtcagg
     3241 ccgacgagga cgccagtgtg gtcttcattg cgcaggccgc gctttacgcc gtacttggag
     3301 aaccattcgc ggggaatctg tgtgtagttt tgtgacttgc cgtaaaactg ggctaagtaa
     3361 atatttttgt ccaatgtgat tcctctcttt cctgcaagta tgaatatggg agaataactt
     3421 ataaagtatc ggttatatgc gaatattcat aaattttatt ttaggaataa tttctagtat
     3481 attcctgcaa agtataagtt attatagcat aattatggag aaagtaaaat aaaaatcgcg
     3541 taaagtgtaa aacgttttaa gggtagccac ggccgtttta attatgatat aatatttatt
     3601 gaaatgctta aaattttata ttgtgaaaat ggattgcaat attttcacaa tgagaaaagc
     3661 catcgcccag gctggcggtg acaaaggagg gcttggaatg attcagttat atgatggagg
     3721 cgcatatctc gtcaacggga cggacatcgt gccggaacag gatgcggcca aactcagaca
     3781 gctcacaggc aggaccgttt ctaaggaaga agggcggaaa gggaccatcg cctattctat
     3841 cttaaaaaat cataatacgt cgggttccat ggaacacttg aaaatcaagt tcgacgccat
     3901 ggcttcccat gacattactt ttgtcggcat catccagacg gctaaggcat cgggaatgga
     3961 aaaattcccc cttccctacg tgctgacaaa ctgtcataat tcgctctgcg ccgtcggcgg
     4021 caccatcaat gaagacgacc acgtcttcgg cttgtcggca gccaagaaat acggcggcat
     4081 cttcgtaccg ccccacgtcg ccgtcatcca tcagtacatg cgtgaaaaga tggccggctg
     4141 cggccgcatg atcatcggct ctgacagtca tacccgctat ggtgccttag ggactatggc
     4201 cgtcggtgaa ggcggcggcg aactggtcaa acagctcttg tgcgatactt atgatatcgc
     4261 ctatcccggc gtcgtcggcg tctacctgac gggcaagccg agtccggccg tcggcccgca
     4321 tgacattgcc agcgccatcg tcggtgccgt cttcaagaac ggctacgtca agaacaaggt
     4381 catggaattt gtcggccccg gcgtcgcttc catgacgacg gactaccgca acagcgtcga
     4441 cgtcatgacg acggaaacga cgtgcctgtc gtcggtatgg cggaccgatg acgacaccaa
     4501 ggcctatttg acccagcacg gccgcggcga tgcttataaa gaattgaatc ccgccgatgt
     4561 ggcctattac gacggcatga tctacatcga cctgagcacc atcaagccga tgatcgccat
     4621 gcccatgcat cccagcaacg cctttaccat tgatgaactc aatgccaacc tggaagatat
     4681 cctccatgaa acggaagaag ccatcaacgg ccgcatgagc aacaaggatt tgaaatacag
     4741 cctgaccgat aaaatcaaaa acggcaagct ccaggtccag cagggcgtca tcgccggctg
     4801 tgctggcggc aactacggca acgtcatggc cgctgcccat atactcaagc aggcttcctg
     4861 cggcaacggc aacttcaccc tcgacgtcta tccgtcgtcg cagccggtct atctcgacct
     4921 ggtccgcaag ggcgccgtgg catcgctcct ggaagcgggc gccatcttca agacggcctt
     4981 ctgcggtccc tgcttcggtg ccggtgacac gccggccaac aacgccttca gcatccgcca
     5041 tacgacgcgt aacttcccga accgcgaagg ctcgaaaccg ggcaatggcc agttcgcttc
     5101 ggtagccctc atggatgccc gttccatcgc tgcgacagca gctaacggcg gcgtcctgac
     5161 gtcggctgaa ggctatatgg acgactatgt cgaaacgccc tatgattacg acgaccagcc
     5221 ctacgctacc cgcgtctatc agggcttcgg ccaggccgac gaaggcgctt ccctggtcta
     5281 tggtccgaac atcaaggatt ggccggatat ggaacctttg gccgattcgg ttttgctcaa
     5341 ggtctgctcg aagatcatgg acccggtcac gacgacagac gaactcatcc cgtctggcga
     5401 aacgtcgtcg taccgctcca atcccctggg cctggctgaa ttcaccttgt cccgccgcga
     5461 tccggaatac gtagaccggg ccaaagccgt agacaaagtc gaacgggccc gcctggccgg
     5521 cgactgcccg gctggcgtcg acccggccct gaaagatgtc ttcgccgtcc tgcgcactca
     5581 ctttggcgac gtttccatga aagacgtgga aatcggcagc atgatctact gcatcaagcc
     5641 cggcgacggc tcggcccgcg aacaggctgc cagctgccag cgcgtcctcg gcggcctggc
     5701 caatatctgc caggaatacg cgaccaagcg ctatcgttcc aacgtcatca actggggcat
     5761 gctgcctttc cagatggaag gccaaccgga ctttgaaatc ggcgattaca tctatgtacc
     5821 ggacgtcaaa aaagccctcg acggcgacat gagagccatc aaggcctatg tcgtcggcaa
     5881 ggacctgcgt gaattgtcgc tgtacatcgc gcccatgacg gacgatgaaa agaaaatcgt
     5941 caaagccggc tgcctcatca attacaaccg gaaccgctga ttattcgtcg aaagtatcgg
     6001 ggaaggcgcg gatgccttct ttcggtgacg tgtgatagtg gtttttccag gccctgtaga
     6061 atgtcgaata cgtcgtgaat ccgcagtcat agcagatttc cgtcaaagcc cgcccctcct
     6121 gcatgaggtg gcgggctttt tgcagccgtt tcgtcgtaat gtagcgggcg gcggtcatgc
     6181 ccgtttcggc tttgaagaga tgcatcaagt agtccgggct catgaaaaag gcggcggcca
     6241 ggctgggaat ggacaggtcg ccgctcaggt tggcttcgat gtaagccagg atgcggcgta
     6301 tcttctcgtt ctgatggccc ttctggacga tgcggagctt gtctttctgc agggcccggg
     6361 tcaggtggat catgaactcc gtaaacagcg tttcctgtaa gagcgggctt tccggtccca
     6421 tgtccgacca ggcccggcgc aggcggcacg atacattgta caagttgtcc gcttcctggg
     6481 gctggcgcag gacgggagcc ttgggcgccg tgaatatctg gccggccggg cagccgcggc
     6541 gttcatagcc ctgcagataa gccggggaaa tataagcgat gatgcgttcg tagacatcat
     6601 cgtcatagat gatagggcgg tggatgcggc cggccgggat gacgacgatg tcgaaaggct
     6661 gcaagtcata ggccgtccct tcgacgtcat acgatacgtg gccccggaaa aagagggtga
     6721 tcttgtcgaa gtcgtggtaa tgataggggc aggtccactg ccgcttatcc gatagatgga
     6781 agagttttaa tttttccgct aaataacccg tcttttgcgc catacctgtg tcacctcaca
     6841 ccttatctta tagcaggatt tgcaatataa agagcattat ctacatatat ttgccacttt
     6901 ccgctattgt ataataacaa ctgtaaggat tcaagtaagc aatgatagta gtatagatat
     6961 tttttcacgg agggtttaac tatgaaattc gtaaaaatgc acggcttagg caatgacttc
     7021 gtctttttgg aagataaaaa cggcgcggac aaggattttt cccagctggc cgtcaagatg
     7081 tgcgctcccc atacgggcat cggcgccgat ggcatcatcg tcatcgtacc gagcgataag
     7141 gccgacgtcc gcatgcgcat catcaatgcc gatggcagtg aagcggaaat gtgcggcaac
     7201 ggcatccgct gctttgccaa atacgtttac gacaacggca tcatcgacaa gaaagaattc
     7261 gccgttgaaa ccctggcagg catcatgaag ccgaaagtca ctgtcggcga cgacggcaag
     7321 gtcagcctgg tcaccatcaa catgggtaag cccttcacgg accgcgccca gatccccatg
     7381 gaaggcccgt ccggcccggt catcgatgaa cacatcgaaa tcgacggcaa gacctataac
     7441 atcaccagcc tgctcatggg cgtcccccat accatgacct atgtcaaaga cgtcgacgcc
     7501 gtcgacctcc acgaattagg cccgaaattt gaaacctata aggctttccc gcgcaagacc
     7561 aacatgaact tcgtccaggt catcgacgac cacaccatca aagtccacac gtgggaacgc
     7621 ggtgccggcg ctaccctggc ctgcggtacc ggctcctgcg cctgcgccgt cggctccttc
     7681 ctgaacggct tcactggccg cagcgtcgac gtccagctct acctgggcac gctgcacatc
     7741 gaatacaacg aacaagacgg caacgtctac atgaccggcc cggcagccgt caccttcacc
     7801 ggcgaatggc tggaagcgta ggggcgccca cgtggggcgc ccgcccggaa tgatggcgat
     7861 aatgtcatac atgcattcgc aggtcgccgc ccgtaggggc tcgcacgtgg agaatcatcc
     7921 caattccatg acgggtcgcc cacgtggcga cccctacact gtaaaatacg aatcactaac
     7981 cactaaaaag gggggctgta acaaaatgag tttttttgct cattttgttg cagccttcct
     8041 tctttc
//
LOCUS       DAWCYS010000052         8035 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_580_length_8035_cov_6.542105, whole genome shotgun sequence.
ACCESSION   DAWCYS010000052 DAWCYS010000000
VERSION     DAWCYS010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_580_length_8035_cov_6.542105"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1599 a         2174 c         2475 g         1787 t
ORIGIN      
        1 atttctttcg cttctgccag ggacgtgccg acaggagcag tgatcaggtt ttctttggtc
       61 atgatgtcac cgatgcggcg gctcatgtct tcttcaaagc gtatatcgcg gttggtgatg
      121 atgccgacga gtttgccgtc gaccgtgata ggtacgccgg agatgcggta tttgcccatt
      181 aattcattgg catcggccag gagattatcg ggatgaagga aaatcgggtc gatgatgatg
      241 ccgtgttccg accgctttac tttgtcgact tcccgcgcct gggcggcaat ggacatgttc
      301 ttatgaataa cgccgatgcc gccttcgcgg gctacggcaa tagccatagg agcttctgta
      361 acggtatcca taccagagct cataatagga atattcagtt tgatatcgcg cgtcaggttc
      421 gtcgaaacgt cgacttcctt cgggagtacg tccgattttg ccggaaccag gagaacgtca
      481 tcaaatgtca aacctctcat accaaattta tcagatctca tatttgcacc tctgttttga
      541 aaaatataag aaactaaggg gaaatacccc taaatagctt atcccctatt ataactgaaa
      601 tcagctctta tgtacatata ttacagcaaa tttctatcag ttatttaaaa ggccggcaaa
      661 agtatggcca aagcgtctct ttaatgccgc ttctgctttc cccaggtctt cgtggcccag
      721 ccaccattgc cgggccgcat cgcgggccgc cgccaggaga ggtacgtcgt ttataatatc
      781 ggcagctttc aaatcgggca ggccatgctg catatagccg aagagctgtc ccgaaccgcg
      841 caggagcagg tctttttcag ccagcgtgaa gccgtcgtgt atctgttcca tccatttcag
      901 gcgctgccgc gtctcatcgc tgccgccttt ggccatgagg atgcaatagg cctgcagatc
      961 gccgcggccg acgcggcccc gcagctggtg gagctgggcc aggccgaagc gttcggcgcc
     1021 gtcgatgaac atgaccgtcg cattggggac gttgacgccg acttcgatga cgctcgtcgc
     1081 gaccaggagc tggatatccc ctttctgaaa ggcctccatg acggcttctt tctctttgcc
     1141 ggccatacgg ccgtggacga ggccgcacgt gcgctttttg aagacgcggg accgcagttc
     1201 ttcgtagagg tccgtcgccg cctgtaaatc gacgctttcc gaggcctcga cgaggggaca
     1261 gacgacgtag cactgctggc cctgggccat gagcttgtcc atgaagatat agacccgccg
     1321 gcgcagggct tcgccgacgg cataggtctt gacccgtttc cgccctggcg gcagctcgcg
     1381 gatggacgac acgtcgaggt cgccgtagag ggacaaggtc agcgtccggg gaatgggcgt
     1441 ggccgtcatg aacagggcat gtggctcctt ccccttttgt tccagcgccg accgctggcg
     1501 gacgccgaag cggtgctgtt cgtccgtgac gaccaggccc aggcagcgga aggccacgtc
     1561 gtcctgtatc agggcgtgag tcccgatgag gatgtcgatg cgcccctggg ccagttcttc
     1621 caggagctgc tgccgttcct tagccgtcgt ccggcccgtc agcaaggcga tgcggacgtc
     1681 caggccctga aagaggcggc agaattcttc atagtgctga gccgccagga tgcccgtcgg
     1741 cgccatgagg gccccttgat agccgttctc tacgatcttg gccagggcca gggcggcgac
     1801 gacggtcttg ccggagccga catcgccctg gaccagacgc tgcatgggca cttcgctctc
     1861 catatcggcc tggatatcga ggaaggcctg ggtctggcct ttcgtcaggg taaagggcag
     1921 gtggtcatag aaggccttga gcagatggcc gttagggccg catttgatgc cctggcgctt
     1981 gccttcatgg cgccggcgca ggagcaggcc gagctgcatg tcgaagagct cttcaaaggc
     2041 cagctggcgc cgggcctgtt cccgtttctc catagaatca gggaaatgta tctgctcata
     2101 ggcttcgagc gtcgacatgg cggcaaaagg cgactgacgg cagccgtccc agccgggcag
     2161 gatgtccgga tgttccctgg ccaggtccag ggcctggcgc acggccttgc ggatatcctg
     2221 ctggcgcagg ccgtcggtca ggccgtagac ggggacgaag ccatcgccgc cagcccgccc
     2281 ttcttccgta tcgggcgtat tcatctggcg gcggccgtaa gcccattcca tcttgccgaa
     2341 agccgtcagg gtcaggccga catggaactg gcgtttcttg aaggcctgat tgaaccagac
     2401 cagttcggcc gcccccgtgc cgtcgctgac catgaccttc aagatggaga ggccccggcg
     2461 gggccgcatt tcctggacag aggccacggt cccgcagacc aggacggctt tccccgtccc
     2521 gcacggcgcc tggccgatgg gccagatgtg gctgcggtct tcataatcgc ggggcatata
     2581 ttgtaataaa tcggacaccg tcgtcagatg gagccgggca aacagggctt ctttgcgcgg
     2641 tccgatgcct tttaaatcgc gaatggaaaa attcatacca aaaactcctt gtcaaatatt
     2701 cgtttttatt gtatcatatt tcgtaggctt ttttaaaaaa taaaacttgc tttgcctaat
     2761 aatctgtgat aagatttata tgttgtactg tgatggtgca gggaggtgta accaatggca
     2821 aacttttgcg aaatctgtgg taaaggaaca atgagcggca tgaatgtcag ccattcccac
     2881 ttgaaaacga aaaaaacctg gaaaccgaat atccagcgtg tccgcgctgt cgtcgacggc
     2941 gaagtaaaac gagttaatgt ttgcactcgt tgccttcgtt ccggtaaagt ccagcgtgat
     3001 gtttaaggat gataaaaaag aggctgtcac attaagacag cctctttttt agtttaaagt
     3061 ttgtagtttt tagtttgcag tgaatgttta aaaatttaaa gctgtcatct gcaaactaca
     3121 aactccgtac tacaaactat aaagagaaag ggacctcgca tgatagaata aaccttcatg
     3181 cgacagtccc ttttttcgtg gttcgtagct cgtagggggc gccacgtggg cgacccgttg
     3241 ggaatttgtg tgatttcccc aacgaaatga acggggaatc gcacatatcc tggagcgggc
     3301 tcgccacgtg cgagccccta catgcatatg tattttgcca ggcgggccgc ccggtcttcg
     3361 atgccggcgt tccggaggat gctcttgggg tcgttggccg tttcgatgag ggcgaagtac
     3421 ggcggcagga tgaagttctt gttgcagttc atggcgtcca gtatctgttc ggccacgatg
     3481 tcgccgccgc tgtagccgga gacgaccagg gcgtaaactt ccttattggc aaaggccgcc
     3541 tcttccttgc ggaacagggc cgtcagcctg ttgaagaagg ccgtgatgtt ggcgctgacg
     3601 gcgtcgttgt agttcgggca gaccaggacg atgacgtcgc cctggcgcac agcgggatag
     3661 actttttcga ccatgacgcc gccgtagaag cagtcccctt tttcgccgaa gtggaggcac
     3721 gtttcgtagc tgcagccccg gcagtcgacg acgctgccgt tgcgcaggga aatctccgtg
     3781 accttcgcct tcccctgcag atggcagcgg accatctccc agaggaggag cgtattcgac
     3841 gtgcggcggc tgctggcgtg gatgacgacg atatctttga ccttccctga cccggacggg
     3901 cgaacgaagg acagcagctt gttgaccagg tggccggcac tgacggcata ggcctcttca
     3961 ttagagaggc cctggatctt ggcctgggta ttaaaattat agagagagcc cgtagcctcg
     4021 acgagaggct tcccgggaaa gatacagccg gcctgattgg caaagaaaat cagctcccgg
     4081 cctatttttt ttgtaaaaaa ttccccctgc ccgtcgacga ggacggcacc gcggcagccg
     4141 gccaggcagt gacggtggcg gaccaggcag gatacgaggt ggacatagga caggctgacg
     4201 ccggccctgg gcagggcgac ggcaaagagg acggctgtcc cctctttttc cgccggcgtg
     4261 cggacgcacc agcgcaaaaa ggccttttcc cgtttgatga cggtatacgt ccacccggcc
     4321 agggcccggt cgaggacgcg ccggattcgt tccgtgtggc ccgcttcctc ccagccgacc
     4381 ttgacgacca acaacatggc aaggccccct tcctatacga ggcggctcag tgccgtataa
     4441 gcccgtttcc agcgcgcata gagggccggg caggcacaga cgtcttcata ggtcacgcag
     4501 ccgccggcga cgcggttctt gacgccgaag aaagcgcctt tcgatgtatc gccgaggtag
     4561 accgacccgt aatgccactg gccgcgcagg gtcaggatga tcgacaaggc cgccgacgac
     4621 agggccgggg cgatgtaggg cttgtagccc aggtcgcgga cggcgacgtt ggcatggacg
     4681 acgaggtccg tcagttccct ggacagcgcg tcgtcatagc cgtcgaggcg gtcggcgatg
     4741 accaggtcta ccccgtgggg tccaaaggcg cggccttctt gcagataatg ggcgaagcgc
     4801 ggatcctttt tcgcatagta gcaggcccgg gcatgcatga cgcccaggcc gtagccctgg
     4861 atctgcgacg gatggagacc ggaagcggtc aaaaaggcgc ggcacaaggg gtcgaccgga
     4921 tccgatacga tgcagacctg gccgccgaag ccggcctggc gggccagggc cgcataatgg
     4981 gcgacgagac tgcggttggc ttcgagctgg gccatgcgca cgtcgccgcc ggcgccgaca
     5041 ggcggtacgc ccttgctggc acagaaaatg aagacatcgc agtcgaagac gtctttttcc
     5101 gggacgatgg tcacggccgg caggaccggg tcgtcacagc catccagtcc ggcaaagggg
     5161 tagcgtatct ggttcatctc catttccagg cgggccaggt tcttctcgtt gatatcgcac
     5221 atgccgacag aaccgatgac gtcctggccg gccaggagca ggccgatgag catggtcgtg
     5281 cccacatcgc ccaaggcgac gatatggacg cgggccttcc cggccagctg cggtgccctg
     5341 tcgaggggca tggcagaggc tccgatttct ccgggcagat catgtatcca tttattcaag
     5401 ttctccatct cctttccccc agcgcaggcg gatgagccgt tcgatatcgt tcttgatgaa
     5461 gcgcgtcggg tccatgttct catcgaactt gacgccgacg tcgatatcgg ggatgcgcgg
     5521 gtaggtaatg acttcgccga gccgcttcag ggtcaggcgt ttttcaaagg tttcctggta
     5581 gacgtcctgc aatgtctcca tgatagatgc ggcattttgc agacacgtca gggagtataa
     5641 gtagtcggcg tcgctgttgc ccttgccgat gaaagccggc ttgacatagg gcaggaccag
     5701 gttgatcgac ggattgcggc cgtagccgct gtagttatgg gcattgtcca ggctgtcgcc
     5761 gccgatgaag ggatagcagt ccctttcgtt gagccagact tccaggctgg gcaggaaata
     5821 agcgctctcg gcgcggatgt ccttttcttc catcatgtcc atggcctgac cggtcatgac
     5881 gccgaccagg acttccttga tttcgacgcc gttctgttcc aggtagggcg tcagcatgcg
     5941 catgcggtgg cctttgtgca ggaggtcgtc gatgaggatg accggcctgt cgaaggactt
     6001 gatggtcttg acctgattgt cgacggacga atagtgctga cattcggcga tggtaaaaga
     6061 cttgaccgtg cggtcgaaat acttgtcgat gtgcaggccc ttggtcaccg tgttgggtac
     6121 gaggacatcg ctcagggcct tgccaaaggg aacggtcatg tacgggccgc ggcgcttctt
     6181 cttgtcttcg acgatggaaa cgccgttgat ttcagcgact ttcttgatga tcttattata
     6241 aaaggcactc atgttgaaag acaggatgag ctggcccgga taaatgtggt tcatgaccga
     6301 caacagattg ttgtgggcct cgtagatggc ccggcggacg cggggattct tgttgaaggg
     6361 gttcttgatg gtcgtttcga cgtcgcggaa aatgacgatg ggtgatttca tgttgactgc
     6421 ataaatcggc gtctcttccg gcggcgcaat gttgacgaac ccctggcggc gcaatgcgtc
     6481 gatgacgggc tgctcgcagc cggccggatc gacgggatgg tagacggcgt aggcgtagtc
     6541 gcgggcaatg agctctgtca ggatttccgt aatgatgatc tggctgacat tggcaatgcc
     6601 ggccatgtcg tcggcataga gaaagccgat gcaggcaatg cctccgtcag cggcatcgcg
     6661 gatgtgcgag gcaatggccg agtcgtggaa ctcgcgcagc aaatcgcggg agcccacgcg
     6721 gtggacggcg gcgtaagcga tcatcttgcg ctcctggctg gcgtcgtcga tgtacagcgt
     6781 ccagacatag tccttttcga cgtagctgtc gacggtctcc gtatggtagc ctttatccca
     6841 gagggcgctg cagatgggcc acaggctttc ggcgccgcgc ggcttgaaag agccgatgcc
     6901 gatttcccga gcttccagga cgtgcttgta cgccggttcg cgcaggtaaa ggtgggtatc
     6961 gtaaatgaag ttctgtgcca cggcgtcgat gaggttggaa atatcccggt taaggtcgat
     7021 gttctcgcgt atcttcgtcg agctgatgtc ttcgtagaac ttgtccagcg tcaggcggat
     7081 gaccttggcc tggatgccgt aggtcttttc atgctggtcc ggttcggcat tttcccgcgt
     7141 ctccctggcg aaggcgatat ggttgaccgt atggatcgaa tccggcgtcg gtacggcccg
     7201 gtaagccgac gcattttcga tgacgtccgt gccgacggcg atgtacaggt cccggccggc
     7261 aaagatgtcc ttgagcttct tgatgtccgc cggattggcg atgttgatgg ggatatcgtc
     7321 cgggaaggga tacatgttct cttcatcggc gacggatatg ttcatgatct tgcggcgcat
     7381 gagccgcggc tgggtgtgct tggaccagga aaattcatcg agggccaggt aaatgtcgaa
     7441 gcccaggtcg cgtatcttgc aggccacggc cttgtggccc aggctgaagg ggtcgaaggt
     7501 ccccggatag aagcagggct tgcggcctgt ttcaaaggta aagggccctc tggcgaattc
     7561 gtgctggctg atgtagcggt aaatatggtt gagcacggcc gaattactgt agaaatccag
     7621 gttttcttcg ctgttctcat cgaggaggac caggagtttc ttatagcagt ggctgaagag
     7681 gaaatctttc cgttcgtccg gcatgagcgg gctgtggaag atgaatttcc ctaaatcgcg
     7741 aaaggcgtcg cggctcagtt ccttattgta atgggcatag gccttgatca tgatgtacag
     7801 caggcggcgc tggcgctttt cgttgaccgc cgggtcttct gagaactctt tggcaaattc
     7861 gtcgaattgt tccaaaatga tgcccactgt ctggaccatg gacgctgcca aggggatgtt
     7921 gaccgtcagc agctggcctt cgatggtctt gacgaattcg tcgcactccc gcggcggcag
     7981 cttgaggctc atcttgccca acacttccgg cccggagtta gctgccgggc ggtcg
//
LOCUS       DAWCYS010000053         7973 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_585_length_7973_cov_7.302349, whole genome shotgun sequence.
ACCESSION   DAWCYS010000053 DAWCYS010000000
VERSION     DAWCYS010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_7973_cov_7.302349"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1770 a         2044 c         2293 g         1866 t
ORIGIN      
        1 tgatgaagga acgtccgtcg ttgaaggtgt gaccgtcagc aggcagttgc tgctgcacaa
       61 gacgcagttc tggatggatg gctggacctg caattcttcc agggctttcg ggctgacgat
      121 ggtcgatttg gcatggtctt cgtggtaggc cgtgcggcag atgaggccca tgccgaaggc
      181 ccccatgagg ccggaaatgg ccgggcggat gacgtggcgg cccaggagtt tttcaaatgc
      241 ccgcaggacg gcgtcgttgt agaaagtacc gccctggacg acgatgtgct ttcctaattt
      301 ggccggatct ttgactttga tgactttata aagggcattc ttgatgaccg aataggaaag
      361 gccggcggaa atgtcggcca gcgtggcccc ttctttctgg gcctgcttga ctttggagtt
      421 catgaagacc gtgcagcgcg agcccaggtc aatggggctc ttggccagca gggcggcctg
      481 ggagaagtcc tggatggaca ttttcaggga tttggcaaag gtatccagaa aggaaccgca
      541 gcccgacgag caggcttcgt tgagcaggat atcttcgata tgaccatctt tgatgcggct
      601 gcatttcatg tcctggccgc cgatatcgag gaggaagtcg acgtccgggc agaagtggga
      661 cgctgcctga tagtgggcaa tggtttcgac ttcgcccagg tcgaggtgca gcgcttcttt
      721 gagcagcgat tcgccgtagc ccgtgatgcc ggccttggcg atgaaggcct tttccggcag
      781 gcggcggtag acttcagcga cgattttctt ggctgccgcc aaggggctgc cttcgttgtt
      841 ctgatagtgc gaatagagga tcttcatgtc ttcgctgagg acgacggctt taatcgtcgt
      901 cgagccggcg tcgatgccca ggtagcacgg gccgctgtag gtggtgatgt ccgcctgggg
      961 gacggtatgc tggctgtggc gggcccggaa ttcgtcgagc tcagcctgat tgttgaacag
     1021 cggttcgacg cggtccgatt tggccagcgt cgattcgtcg acgctcttca gctggcccat
     1081 gaggttcatg aaggacacgg gtttcgtgtc tatactgccc agggcggcgc cgatggccac
     1141 gtagacctgg gcgttttccg gggcgatgac ctgttccggc gtcaagtgga gcgtcttggc
     1201 gaactgttcc cgaagctggg acaagtaggt cagcgggccg ccgaggaagc agactgtccc
     1261 cttgatgggg cggccgcagg ccaggccggt gatggtctgg aagacgatgg actggaagac
     1321 cgacgccgcg acgttttcct tgctggcccc ttcgttgagc agagcctgga tgtcggtctt
     1381 ggcgaagacg ccgcagcggg cggcgatggg gtaaattgtg tcggctttct tggccatttc
     1441 attgaggccg ccggcatcgg tgtcgaggag ggtcgccatc tggtcgatga aggccccggt
     1501 cccgccggcg cagttgccgt tcatgcggtg ttcgttgccg ccggtcagat aggtgatctt
     1561 cgcatcttca ccgccgagtt cgatgatgac gtccgactgg ggataatacg tgcggacggc
     1621 tttggtaccg gcgatgactt cctgcaaaaa ggggatgtgc aggtagtctg ccagggcgat
     1681 gccgccagag ccggtgatgg ccatggtgat ttgacggtcg ccaaagattt cataggcgtt
     1741 ttgcagtaaa tcccagactt tttggcgtat atccgtatag tggcggacat agcatgcgtg
     1801 gaggcactgt ttggcatgat tgaggacggc gatttttacc gtcgtcgagc cgatgtcaat
     1861 gccgatatgt aagagatttt tcatgttttg tcaccctatc ggtttaccaa ccgattcttt
     1921 cttataaatt tctatatgat aaagactata tataaagagt ctactttata ttgtaccata
     1981 ttcctcttat tctgtaaggg gtggggcaga ggattttttt acgacccaaa atgagttacg
     2041 acaaatggtg gtaaatatgg tagaataaaa gtgtcaatgt cttaatgaaa actatacttt
     2101 atatgataaa ggagccttgt catgagaaaa ttcgtaacgg aacatcagtc cccctacctg
     2161 gatttgacga cggaagtgtc ggatatcctc taccgcggcc attcacagtt ccaggatatc
     2221 atcgtcgccg attctaagga attcggccgt atgctcgtcc tggacggcgt tttccagacg
     2281 tccatcaaag atgaattcat gtaccatgaa agcatcgtcc acatccccat gttcctccat
     2341 cccaatccca agaaagtcct catcatcggc ggcggcgatg gcggcgctgc ccgggaagcc
     2401 gtccgccatc cggaagtcga atcggtcacc atggtcgaca tcgacggcaa ggtcatcgaa
     2461 ttatccaagc agtatttccc ggaaatcgcc aaggccatga tcgaaggcga cccgaaactg
     2521 actgtcaaag tcggcgacgg catcgcgttc atgcgggaag cagaaaatta ctacgacgtc
     2581 atcatcgtcg actgctcgga ccctgttggt cccggcgaag gcctgttctc gtatgatttc
     2641 tacaaggata ccttcagggc cctcaaagac gacggcctct tcgtacagca gaccgaatcg
     2701 ccgttcatgc accgcaagct ggtcaaggat atcttcgact gcgtgtcgga catcttcccc
     2761 atcacccgcc tgtacacggc cttcatcccc ctctatccgt caggcatgca ctgcttcacc
     2821 atgggctcga agaaatacga tccccttacg tggcagccga accgtaaacg gaccttcccg
     2881 acgcggtact ataacgaagg catccagcag agcgccttcg tcctgccgaa tttcgtcaaa
     2941 gatttattgt acggtgaaaa agaacgctaa aaaagtttga aaaagtgctt gactttgtga
     3001 gcgactcgtg gtaagattat ctacgttgac ggcgctaatg cagcgcggcc gacgcagata
     3061 atctaatatg gcgggtatgg tgaagtggtt aacacggcgg attgtggctc cgttacgcta
     3121 gggttcgaat ccctatactc gccccatagg ggtatagcca agtggtaagg caccggactt
     3181 tgactccgct atgcgctggt tcgaatccag ctacccctgc cagccatgat tcattagctc
     3241 agctggtaga gcacctgact tttaatcagg gtgtcccgag ttcgaatctc ggatggatca
     3301 ccagttaaaa aagctgtcgc ttttgcgaca gcttttttgt gtttgaagtt tgtagttttt
     3361 agtttgtagt ggatggttaa aaatttaaaa ccatctgctg caaactgcaa actccgaact
     3421 acaaactata agataaaagg gactacgcat aatgacataa gcctttatgc gatagtccct
     3481 tgtttacgtt ctgatggaga aatagcgagc agccagcgtc agccgcccgc tgggacggcc
     3541 cctacaaatg cggtccatgg gaccgctagc cactaaccac gagccactag ccacaactta
     3601 caggaagaaa acgacggtgt cgatgatgaa caacgggatg aggatggcga cggaccagcc
     3661 catgtagccg aagaagctcg gcattttgat cttgttttct tcagcaatgg atttgaccat
     3721 gaagttcggt gcattgccga tatacgtgtt ggcacccatg aagacggcac cggccgagat
     3781 ggcttccagc atgagctggg atacggtccc gacagaggtc gtgatgccct gggtagcgcc
     3841 gagggcaccg gccatctgga ggaagaccag gtatgtcggc gtgttgtcca ggaaggacga
     3901 cagggcaccg gctgcccaga acatctgcca cggatgggtc agacccagtt cagcgccatg
     3961 ggccttgagc aggatcaggg ccgggatcat ggtgatgaaa atgccgatga acagcttagc
     4021 gacttccagg ataggggcat acgtgaattc gttgagttcc cgcgtcgacg tcttcgtcgt
     4081 cttgatggag aggaaggctg cgacgaggat gatgacgatt tctgccagtg tagcataggg
     4141 gaagacgatt tcgtcgtaaa tgtgcaggcc agcgccgttg gcgaaagccg ggaacatatc
     4201 cggcaggagg ccgttggcca ggacgccgac gatgatcatg gcgatgaaga tgaggttgtg
     4261 ggccccttcc aggcggaggg gctgcttgtc gttttcatcg atcaagtcga gcggcgtacg
     4321 gccttctttt atttccttat tataaaaatg cttgtcgatg acggcgaata aggcgaacat
     4381 gaggaccagg ttgaaaatca ggatgggcag gagatggaag gtccaggtaa aggggacgcc
     4441 gcgctggaag cccatgaaca gcggcgggtc gccgagcggt gtcagacagc cgcccatgtt
     4501 ggcgacgagg aagatgaaga acaccatgag gtgtactttg ttcttgcgcc aggcattggc
     4561 ccggatgata ggacggatga gcagcattgc tgcgccggtc gtaccgatcc agctggccag
     4621 gaaggtgccg atgaagagca ggagacagtt gattttcgtc gtcccggcta aggtcccgca
     4681 gacggcgata ccgccggcga cgacgaacag gccgaagagg aggacgatga aggggatgta
     4741 gtcgaggagc accgattcga gcaggcggaa ccacgactca ccgatgccat aggcagcggc
     4801 gaaggggatg aggaataaaa gagaccaggc gaacgcagcg ccgagcatgt gtttttccca
     4861 ccattccgga cggacgaggg ggacgatggc gatggataag agcatcccgg cgaatgggat
     4921 gatactccat acgggcaagg tcgcacttac gagttcatgt gtcataaaaa ccacctttct
     4981 tattatctga ttgcagagaa acttaaaaat tttacaatca tattcagtat tatagcatag
     5041 agacatgaaa gtttatactc atttttcaga tttcgcatag tcatttaagt agaagctaaa
     5101 taattttata aaaataaaaa gcaggcattt tctgaaaaaa attggatgac aaagaaggaa
     5161 tccgtaaaag tcatggcgaa tatatatgtt aaggataaga acaggcctta ataaggaggg
     5221 attcatatga aaaaatataa taacatcatc gtgccgacgg atggttcggt taactcgaaa
     5281 cgtgcgctgg aacatgccgt cgtcatcgct tcgtcgttag gggctacgat tacactggtc
     5341 tatgtcgcca atatcgtatc ggtcatttct aacttcgacc agatccccaa tgccagcggc
     5401 tatgtaacgg aacaggtcgc actggacatg gaagaagaag ggaaaggtat cctcgacgaa
     5461 ttcgccaaat ccattcccca gaacatcgaa gtcaagagtg tctttgaagt cggttctccg
     5521 ggaccggccg tcttgtcggt agccaagaag tacaatgctg acctcatcgt catgggcagc
     5581 cgcggcctcg ggccgttgaa aggcttgttc atgggttctg tcagcagcta cgtcgtcacc
     5641 cattccgtct gccctgtatt gatcgttaaa taagtaatta gtggttcgta gttcgtggct
     5701 cgtggttcgc ggagagacga cctgcgttac tgacccctaa ccactagccg ctaaccacta
     5761 accactaaaa agtagtatga agtttgtagg ggtcgccacg tgggcgaccc gttaggaatt
     5821 tggatgattc atctagcaaa ctgattggaa aattttacat gtcccgaagc gggcgcccca
     5881 cgtggcgacc ctacagaaaa aaggcgctgt cttcatagga tagcgccttt tttctgaaaa
     5941 ttttacgtta cgccataggg gaatcgttct ttttgtggta tactgagtac acaggtatgc
     6001 ctttttttgc ctgattacac aaaacctatt catgagtata gggtcccgga ctgcctcgtt
     6061 ccgggatagg tgcctgggag gcaagtatga aacgaaatac caacgaatgg ctcgaccgca
     6121 aggtcgctga ctgggtttct aagggctgga tcagtgaaga atcccagcaa aaaatacgaa
     6181 aatcttacgg cagggacgaa caaaggaatc ctcttttgac gaccctgcct ttatatgccc
     6241 tgctggccgt catcggcctg gccgctgccg gtatcgccct gatttggggc ctttcccagt
     6301 tctggtacta tgtatcactt acggtccgca tgggcctggc ctgtggcctg ctcctgctta
     6361 ctcagatcgg tgtcggcgcc gccatgttcc aggaacggca ggggagcctc gtcgccgaag
     6421 gcgtggccct agtccattgc ctgggcgtct ttgccgtcct ggcaatggcc gagcagtctt
     6481 tctatatcgg ctgggatgtg acggcctacg tcgccgtctg cgccatcctc tgcctgcccg
     6541 tcgtctatct actgcggtcc attgcggccc tcgtcgtcta tgacctggct ctcttgtact
     6601 ggacggctgc cggcgggcca ctgaatacgc tgggcgggac ggcgctgttg tggattttcc
     6661 tcctgttggc cgtgcccttt tatgataccc tcatcaccat gcgtgatgaa cggcgcctgt
     6721 ctattttttc ctgggtcatg acgattaccg tctttgctgc ttttggcctg gcagccaggt
     6781 cgacggacta tatccctttc ctcatgctgg ggagtttggc cgtcaccatc atgctcgtcg
     6841 gttattccat cgacatccat cagtcctggg gcgtaccctt ccgctggttc ggccgctttg
     6901 ccgctgccgg ctcgctgctc atttcctgcc tgcctgcggc ttggtatggc attgcccggg
     6961 tccatgcctt tcactgggtc acggccttgg tgacgctcgt cctctttgcc gtcatgatcg
     7021 ccctcatggc ccagaccgta aagaatcgct tgtggggacc ggtcctgtac ctggcggtcc
     7081 catttatcct ggcttttgaa acgatcctcg tccgggccgg gctctattcg tcgattcccc
     7141 tggtcctttc gtcgctgtac atggtcgtat taggtttctt tgaaacgtct cagggcttca
     7201 agccgggaca cagcctccat atgaaatttg gcgtcgtcat cttcatcagc ctggtgctgg
     7261 cctttatttt cgggacgcag ttctccccgc tggcaccgct cctggccatc gtcgtcctgg
     7321 ccctgatcat cttccagttc aagcggaccc gcaaggataa ggcggctgcc gccttgcgcg
     7381 ctgcccggcg ggcaggtctg cgccattcgt cgacgcgcgt ccgccgtgaa ggcgaagaaa
     7441 aacaggacga acttccgcct aaagagacac cgtcctggca ggctcaggat gccgccagcg
     7501 atgtcgacac cctggccgag tggatgaaag aggtacgcat cccgtcgccg gctgaattgg
     7561 gactgcctgc cgaaccggca ggggaaaaga cgtccgacga tttgcccaaa gcgccgcccg
     7621 tcccggccga gccggaagaa gcaccgccgg catttacttt cacggcaccc gctgatgacg
     7681 taaagaaaga aacgccggaa acgccggcgt cgccggtcac accgacagtc catgaaaaga
     7741 cggaagccgt gccccagcaa atggctgttc ctcaggaacc ggaagcggaa gaaaagccgc
     7801 gggttacgga atcgccgtgg agctcgatga agtcggcacc gaaacggaaa aaatacttta
     7861 cccactcgcc gtggtctcac caaggaggcg atatgaaatg aaatttccat tgcctttgca
     7921 gggattccac ttttcctttt cactggatgg ccgttatcgc tggatctttt tcg
//
LOCUS       DAWCYS010000054         7924 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_588_length_7924_cov_7.084509, whole genome shotgun sequence.
ACCESSION   DAWCYS010000054 DAWCYS010000000
VERSION     DAWCYS010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_588_length_7924_cov_7.084509"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2002 a         2128 c         2097 g         1697 t
ORIGIN      
        1 ccaataagat gaaagtcgaa atgtacaacc gcttcgccct gcctctggcc agcctggtct
       61 gcgccatcgt cggcgcgccc ctggggatgc agaaacagcg cggcagttcg tccatcggct
      121 ttggcatcag tgtcgtcgtt atctttattt actattccat catgaccctg ggcaacgccc
      181 tgggcaatgg cggcaagata ccggcctacc tcgccgcctt cctgccggac atcatctgtg
      241 gcgtcgccgg cgtcatctta gtctacaaaa aatcgaaatg agggaaaagg cgctgtcgca
      301 tgaaggcagc gccttttgag tattaagttt gcagtttgta gtttttagcg aatggttcta
      361 aatttaaagc catctactgc aaactacaaa ctctgtgcta caaactataa agcgaaaggg
      421 gcctcgcata atggtagaaa ccctcgtgcg acagcccctt ttaagaggct gggagctaac
      481 agccagctgc taacgactag ggcaggacgg cccttgcagt acacggtagt tatgggctat
      541 aatataatga tataaaggaa tgagcaaagg tggtgtctta tggaatatgg atgggtcatg
      601 ctgatcgtct tgtctgttat gggcgggatc attgcctatc tcggcgataa gatcggctcc
      661 cgtgtaggga aacagaaaat taaattattc ggtttgcggc cgaagtatac gtccatcctg
      721 gttaccatcc tgacggggat cagcattgcc gtcatcactt taggcgtcat gtctatcttg
      781 tcggaaaatg tccgcatcgc cttgttcggt atgcaccagc tgcagatgca gaaggcccag
      841 ctcgaagccc agcgggatga actgctgcag caggccaagg acctgggcaa ggaactggac
      901 gataagaacg agctcatcgc ctccaatgaa gaactcctga agaaacagca ggaacagctg
      961 gacggcaaga atgaagaaat ccggacgact cagctggacc tgcagcaggc ccaggccgcc
     1021 cgcgacgaca aggcccgcca actgacggtc atccaggttg ccatggacga agccaagaag
     1081 cagcgcgact cggccctggc cgatagtgaa aaggcccgtc aagacgtagc tatgctggaa
     1141 gaaacgaagc agaagatgat gggcaccatc gacgtcctcg acaagcacat caagctgctc
     1201 aatgaaacga tgaccaatat ccgcgaaggg acggtcgtct tccgcgtcgg cgaagtcctg
     1261 tccagtaacg tcctcaacgg cggcctgagc caggcccaga cgcgggaaga actgagccgg
     1321 gccatgagcc agaccaatac catgatctgc cgtcatctcg gcgtccgcga tgaaaatacg
     1381 gtcctcctct atattgcgcc agacgaattc aacgctactg tcgacgccct gcagaaagcc
     1441 gggaagtcgc agaaactgct ccgcatcatc gccgccggca acatcatgct cggcgaaccg
     1501 gccctggtcc acgtcgaaat gtatgacaac aacctcatct atcaccgcgg cgacgtcgtc
     1561 tacgaagaac ggctgacagc ccaggaattg tcgggcaatg ccgaactgca ggtcatgcgt
     1621 ttcctccata acgtcaatca gcagacccag gccaaaggcc tcctgccgga cccgctgacc
     1681 ggcaatgtcg gggccctgac ggcagtcgaa atgttcgaga ccatcaacaa gataaagaac
     1741 cacagcggcg cccatatctt catcaatgag gtcaccgaag ccgataccta cacggccggc
     1801 cccttgcaga tcgacatccg cgtagaagcc gacgaatgat gggagctgtt acatgaaggc
     1861 ttctgtcatc atgaaaagtc cctttctctt tatagtttgt agtatagagt ttgtagcaaa
     1921 tagttttata attactgcaa actacaaact gcaaacttaa aactcaaaat gaggctgtct
     1981 gaatgtggca gcctcattcc taggaggaga tacattgatc tttgccattg accctggttc
     2041 ggaaaagacg gggactgctg tcgtcgccga tgacggcagt ctggtcacga aggccatcgt
     2101 gccgacgccg gaactgggcg actatttgag tcacatttat gaaaactatc ctttttccca
     2161 tatcgtcatg ggcgatggga ccaatcataa aaagctgcag cccctggtcg agcagtggat
     2221 cgccaggaac cacggggata tcactttttc cctgacagat gaaaaattca ccactgtcga
     2281 agggcgcaag ctctatttta aatatacccc gcgcaagggc tggcgccgtt tcgtgccact
     2341 gtccttccaa tatccgccag aaccggtcga tgacttcgtc gcctggatca tcggccaccg
     2401 ttatttagcg aaacaacgag gagaaaaaat atgaaataca tcttgcgtgc cctgggcctg
     2461 gcccttggcg ctctcatcta caccatcggc ctggacgtct tcctggtccc gaaccacgtt
     2521 atcgacggcg gcgtcgtcgg cattgccctc atggcagcgc aggtgacagg catcagtttc
     2581 agtgtgttca tcatcatcct gaacattcct ttctttatct ttggctataa gaagataggt
     2641 gcctttttca ccatatcttc gctcttttcc atcctctgcc tgtccgggtg gagcacgttc
     2701 atccattacg aacgggtcac gtcggatcct ttcttgtcga ccatttacgg cggcatcatc
     2761 atcggcctcg gcgtgggcct catcatccgc tggggcggtt ccctggacgg gacggaagtc
     2821 ctggccatca tcgccgacag gcgcacgcct ttttccgtcg gcgagacgat catgttcttc
     2881 aacctcttca tcctgggcag ttcgggcttt gtcttttcct gggacagcgc catgtattcc
     2941 ctggtggcat acttcatcgc ctataagatg atcgacgtcg tcaccaacgg cctcgatgaa
     3001 atgaagggga tgctcatcgt tacgggcaag cacgacgaag tggccgactg cctgaccagg
     3061 gatatgggcc gcgccgtgac cctcatcaac ggcgaaggcg cttacaagcg ggaaaagacg
     3121 atgatcctct actgcgtcgt gtcgcgcctg gagatcacca agatacgcga tgccatcacc
     3181 ggcgtcgacc ccaacgcttt cgtatcggtc ttcgatatca gtgaagcaca tggcggcctc
     3241 ttcaagcggc ataaaatgca tggataaaaa gtcaaaaaaa gcttgacata attggcgaat
     3301 ctgctgtatt gacatacagc tcatatttgc tattatataa aataattggg ttatagcgtt
     3361 aacctgaaaa aatgacaaca caggtgatag aacacctatt gggaggtata caatggcaca
     3421 gttaaatttc aatatcgaag acatttgcgg cactttgtcg gaaaataaag acggctggcg
     3481 caaagaactg acgtacatca gctggaataa tcgggcaccg aaattcgacc tccgctcgtg
     3541 ggatccggat tatcaggcca tgaccaaagg tattaccatg accaaggaag aactggaaaa
     3601 actgcgtgat gtattgaacg aaatggattt cgataaatac taaggagtga ttaagtatgc
     3661 tttcggcaat tcaatgagat gaatggaatg aaagcatact tttttgtttt ccttgccaaa
     3721 tttgccaaaa cattatataa tctctagttg tatgtgctgt tagtacttgg taataatttc
     3781 aagggtgaat gaaatggttc gtatagcgag agataagcga catgctttat taaagaagcg
     3841 gattgaagaa aatcctttta taaacgacga agaattggct gaagaatttt ccgtcagcgt
     3901 ggctactatc cgcttggacc gcatggcctt gggaatcccc gaaatgcgta tccgcatccg
     3961 ccagaaggca gaagcggcgc agccgaaact gcgtaacgcc cctgtcgtcg gggagctcgt
     4021 ggactgcatc atcggcaaga atgccatttc aatcatgacg gcaacagacg atatggtcga
     4081 cgaagcacac atcgtgcgtt cccagtgcct atatgcccag gccagttccc tggcgcggac
     4141 ggtattgaat cttcctgtct gcattacagc cgtagccaat attaaatata aacagcccgt
     4201 cactgtaggc gataaactga tcgccaaggc cgaagtcatg cgccagcgcg accagaaata
     4261 ctacgtatgg gtcacgatcc ggaagatgcg gaaagaagta ttccgggcaa aatttatcat
     4321 agaatctttg gaataatgga gcggtaatag aatgaaaata gctgtagatg ctatgggcgg
     4381 tgattacgca cccgaagaaa tcgtcctcgg tgccgtagag gccgtcaaag catatgattt
     4441 tgatgtcgtc ctggtaggtg acgaaaagaa aatacgcggc gtactgagca aatatggcgc
     4501 caaagaaagc gaccgcctgt ccatcgtaca cgccagcgaa gtcatcgaaa tgggcgaaca
     4561 tccggctcag gccatccgcc gcaagaaaga cgcatccatc gtcgtagcga cgcggctggt
     4621 caaagacggc ctctgtgatg ctgtcgtcgc cgctggcagc actggcgccg ctgtgacggc
     4681 ggctctcttt gggctgggac gcatcaaagg catcgaacgg ccctgcattg cgacgcctat
     4741 cccgtcgaag aaggggatca cggtgctcct cgattccggg gctaatgcca acagcaagcc
     4801 gaaacacctc gtcgaagggg ccatcatggg ataccactat gcaaaataca tcctgggcgt
     4861 cgaaaaaccg acggtcggcc tgctgaacat cggcgaggaa gcgtcgaagg gcaatgagct
     4921 ctgccagaag acgtatccga tgctggaaaa tttgaaaact atctctttct atggtaacgt
     4981 agaagggaga gatataccga aaggcacggt cgatgtcgtc gtctgtgacg gcttcgtcgg
     5041 caatgtcatc ttgaaatttg gcgaaggcat ggccatgttc atcatccgcc tcgtcagaga
     5101 tacgatcaaa cagagcggct tcatcactaa actgggggcc ttagcggtct acccggcctt
     5161 gaaatcgctg aagaaacgac tggatttcac cgaatacggc ggtgcgccgc tgctcggtgt
     5221 cgatggcagc tttattatct gtcatggcag ttcgaaagcc aaggccatca agaacgccgt
     5281 ccgcgttgcc ggtgaaatcg ccgaacagga cgtcgtcggc catattcgta aaagtataga
     5341 ggaagaagag gtagaacagt atgatagtga aagctaatcc cgctgggatt cttggtacag
     5401 gtcactgtgt acctgatcag gtatggacga ataaagattt agaaaaaatg atggatacat
     5461 ccgatgaatg gatccgcagc cgtacaggca tcggcgcccg ccacgttgcg ccagaaggcg
     5521 tcacgacgtc ggacctggct accaaagccg ccgaagaagc cttgaagaac gccggcatca
     5581 aagcggaaga cctgaatttg atcatcgtct gcaccttgac gccggaccgg gccttgccgt
     5641 cgacggcctg caaagtacag gccaacctcc atgcggtcaa tgccgctgcc tttgatttgg
     5701 aagcggcctg ctcgggcttt gcctacggtt cgatcattgc ttcccaattc atcgaaaata
     5761 agatgtataa atatgttctg gtcattggtg cggaaatcct gacccgcgtc gtcaactgga
     5821 aggaccgctc gtcgtgtgta ctcttcggcg atggcgccgg tgccgctgtc atcggccccg
     5881 taccggaagg ctacggcatc ctcggcgggg ctatgggagc tgacggaacg ggcggtgatt
     5941 tcctgacgat tccggccagc ggtgtcgaaa tgccgccgac cgatgccctg cgcgaaaacg
     6001 gcatggccta tgccaagatg gacggcaagg aagtctataa attcgccgtc aaagccatgc
     6061 cccatgccgc agaattagcc ctcaagcggt cgggcctgac gaaggaagac atcgatgtcc
     6121 tcgtaccgca tcaggctaat atccgcatca tccagtccgc cgccaaacgg ctccacgttc
     6181 cgatggaaaa agtattcgtc aacatcgaga aatacgccaa cacgtcgggg gcttccattc
     6241 ccctggcttt agatgaagcc atcaaagcag ggatgatcca ccgcggtgat atcgtctgcc
     6301 tggatggctt cggcgccggc ctgacctggg ccagcttagt tatgaaatgg tattgatttt
     6361 tttacgagag gtgtgactga tgatgaaaaa ggcatttgtt tttcccggcc agggtgccca
     6421 gaaagttggg atggtcaagg atttatacga cgcatatccc gttgtcaaac agctttttga
     6481 agaagccgac gacgctttgg gtttctccct gaccaagctc tgctttgaag gtcccgatga
     6541 agaactcatg aagacctata atacacagcc ggctatcctg acggccagca cggcctgttg
     6601 ccgcgtcctc gcccaggaag gactgacacc ggacatcgct gccggccaca gtctcggcga
     6661 atattcggcc ctcgtcgctg ccggcgccct gacctttgct gacgccgtcc gcacggtccg
     6721 cctccgcggc cagttcatgc aggaagctgt acccctcggt gaaggcggca tggccgctat
     6781 cctcggcctg gctgaagata agatcaaaga catctgtgct cagatttcgg ctcagggcgg
     6841 cacggtccag gccgtcaact tcaactgccc cggccagatc gtcatcgccg gcagtaccaa
     6901 agccgtcgaa gaagctgccg ctgccatgaa ggaagccggt gccaaacggg ctatcctcct
     6961 tcacgtcagt gctcctttcc acagcacgct catggaaccg gctgccaaac ggttggccga
     7021 agtgctcgat gacatcacca tttccgatgc caagatcccc gtcgtagcca atgtcaacgg
     7081 ccagatcgaa acatcggctg ccgatatcaa ggccaacctg gtcaaacagg ctgccagccc
     7141 ggtcctctgg atcgactgta ccaagaccat gcaggctttc ggtgccaaaa ccttcgtcga
     7201 agtcggcccg ggcaagaccc tttgcggttt taaccgtaaa atcgaccgca aaatccacag
     7261 cgaaaatgta gaagatattc cttccttaca gaaaacgctt gaatttttcc aggaggttcg
     7321 ataaaatgca cttaacaggg aaaactgcta tcgtcaccgg cggctcccgc ggcatcggcc
     7381 gcgccgtagc catcgccctg gctgaagccg gtgccagcgt cgccatcatt tatgctggca
     7441 atacgaaagc tgctgaagaa acgaagcagg ccatcgaaga aaaaggcgct caggctatgg
     7501 tcatccagtg cgatgtctct gatgaaaaag ctgtagccga catggtgaag gccgtcaaga
     7561 aagaactggg ccgcgtcgat atcttagtca ataatgcggg catcacccgc gacggactgc
     7621 tcatgatcat gaaagaagaa gactggcagg ccgtcctcaa taccaacctc aatggggcgt
     7681 tccactgcac caaggccgta tcgcggctga tgatgaaaca gcgcagtggt tcgatcatca
     7741 acattacgtc tgtcgttggc gaaacgggca atgccggcca ggccaattac gccgctgcca
     7801 aagccggcct cattggcttc accaagtccg tagccaaaga actggcttcg cgcaatatcc
     7861 gctgcaatgc cgtagcaccg ggatgcatcg ccacggatat gacggctgtc ctcggtgaag
     7921 acgc
//
LOCUS       DAWCYS010000055         7690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_605_length_7690_cov_7.645449, whole genome shotgun sequence.
ACCESSION   DAWCYS010000055 DAWCYS010000000
VERSION     DAWCYS010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_7690_cov_7.645449"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3049..3058
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1731 a         2407 c         2097 g         1445 t
ORIGIN      
        1 cgacatgatc gccggcccca gtgaagtcgg cgtcctggcc gaccacgacg ccaatccggt
       61 ctgggtcgct gctgacctcc tggcccaggc cgaacacgac cgccgggccg ccgtcttcct
      121 ggtcacgccc agcgccgcct ttgccggcga agtcgaagct gaagtccggc gtcagctgga
      181 agaactgccg cgcaaggaca tcgctgccga atccattgaa aaacacgcca agttcttcat
      241 cgtcaaggat aaggaccagg ccgtagatat tatgaacctc atcgcgccgg aacacctgga
      301 aatcgccttc agcgacgccg aaacgtgggc caagaaaatc gtcaatgccg gtgctatctt
      361 catggggccc tatacgacgg aacccctggg cgactacttc gccggcccca accataccct
      421 gccgactatg gggacggcgg ctttctcgtc gcccctcggc gtctatgatt tcgtcaagcg
      481 cagcagcctc ctggaatacg ataaggacgc tttcacagct gtcgccgaca aggtcatgcg
      541 cttcgccaat gtagaaggat tgcaggctca cggcctggct gtaaggagga gaatcgatga
      601 ataataactg gcttcgcaat accatccgct cttttgaacc ctatgtcgtc ccggaaatcc
      661 gggagcatac ggtcatcaat gccaatgaaa gtccgtacaa cctctttgat ttccccaagg
      721 tcaaagccga tttcctggaa cgcctggcca agacgccgtc gtaccactat ccggacccct
      781 ttgccgaaga actgcgggca gccctggcga aatacgtcgg ctgccagcct caggaagtcc
      841 tcgtcggcaa tggcggcgat gaaatcatca gcctcatcat gaataccttc ctcaatcccg
      901 gcgatacgct cctgacccat acgccgacct tcgacatcta cggcatcgac gccgaagtcc
      961 tcggtgccaa ggtcgtcacc gtgtccgacc tcgacggcta ccgccgcaat cgcgacggcc
     1021 tcctggccaa agtgaaggaa ctccagccga aagtgacggt cctctgcaac ccgaacaacc
     1081 cgacagggga actgctgccc ctggattacg tcgaaagcct ggtccaggct gccgacaata
     1141 tcgtcgtcgt cgatgaagcg tacttggaat ttgccggcgc gccgagcatc attaccaaac
     1201 ttgcccagta cgacaacctc atcgtcatcc gcaccttgtc caaggccttc ggcctggccg
     1261 gctgccgcgt cggctacggc gtggcccaga aggaagtcat cgacgctttg ggcctgacga
     1321 aactcgtcta caacctgaac atcctctcgc agaacgctgc cctggccgcc atggactatg
     1381 ccgatgacat cctggcccac aatatcccgc cgaccctggc agcgcgccag catttcatgg
     1441 acgccttaaa ggacgtgccg aatcttacgg tctatcccag tgcgaccaac ttcgtcctgg
     1501 tccgcgtccc cgacggcccg gccatggtca aggccctgca cgacgccgac atctgcgtcc
     1561 gctcctataa ggcggcagac ctcaagaact gcctgcgcat caccatcacc acggacgacg
     1621 tcgtccgccg cgtcgtcgcc gtcatgaccg aggaggcgaa gaaatatgcg tagtgcccag
     1681 atcgaacggg aaacggcaga gacgcagatc gccgtctccc tcaacatcga cggctccggt
     1741 accttcggcg gcacgacggg cattggtttc ttcgaccaca tgatgaacct cctggcctgc
     1801 cacagcggca tggacattac tctccacgtc cacggcgacc tcgacgtcga cacgcatcac
     1861 accatcgaag acctggccat cgtctttggc gacgccctga cccgggccct gggcgacaag
     1921 aagggcatca accgctacgg catgttctac tgccccatgg acgaagccct gacgcgggtc
     1981 gtcatcgacc tcagcggccg gccctacctg gtctacgacg tgaaactcgc cgtcgaacgc
     2041 atcgggacct ttgaaacgga aatgctcaaa gaattcctct atgccctggc cgtccacggc
     2101 cgcatgaacc tgcacgtcac caatctctat ggcgaaaatg cccaccacat cgtcgaatcg
     2161 gtgttcaaag ggctgggcca tgccttgaaa gaagccgtcc gcatcgaagg cgacgaaacg
     2221 agtgtcttgt cgacaaaagg ggtgttatga tgaagattgc gattatcgat tatgaagttg
     2281 gcaacctggc caacgtatac aatgcctgga aacgcctggg catcgaagcg gccattaccc
     2341 gcgacccggc tgtcatccgc gacgctgatt tggtcgaact gcccggcgtc ggtgccatcc
     2401 gcgatgccat ggggaactta cagaaattcg acctcatccc gctcctgcag gaacaggtcc
     2461 agagcggcaa gttcttcatg ggcgtctgcc tgggcatgca ggccctcttt gaaaagagct
     2521 acgaaggcgg cacctacgac tgcctgggtt tcctgcccgg cgacatcgtg cccttccaca
     2581 cgaaactgaa agtgccccac atgggctgga atgagttgga attccgccag gaacactggc
     2641 tccagaaagg actgccggct catccttacg tctatttcgt ccattcctac cataaatcgc
     2701 cagtggatac gcccgacgtc atcgctacgg ccgattacgg ccagcccgtc ccggctatct
     2761 gcggcaaaga caatgtcctg ggcttccagt tccatccgga aaagagcggc cgcgtcggcg
     2821 aacagctcct ccggaacgtc gtcgattacg tcatgaagaa agggtgagac tatgatcgta
     2881 tttcccgcta tcgatatcca gaacggccat gctgtccgcc tgcgccaggg cgtcaaggac
     2941 gacagtacga cctattttga gaaccccgtc gacgctgccg tcaaatggtg cgacgaaggg
     3001 gccctgtttc tccccgtcgt cgccctggac cgcgccttgt cgggccaann nnnnnnnngc
     3061 gaagaagcta tcgaaacgta tctggaagcc ggtgttaacc gcgtcatcat cgggtccaaa
     3121 gccgtcgaag acccggaatt cattacggct atggccaaga aatacggctc gtccctggcc
     3181 gtatccatcg acgccaaggg cgatgtcgtc gctacccgcg gctgggtcga cggcagtgac
     3241 aagaaggtcc tgccctttgc ccagtccatg ctcgacatcg gcatcaatac cctggtctat
     3301 acggacatca gccgcgacgg catgctgaca gggccgaact ttgaaatgct cagccagctc
     3361 cagcagctgc ccttcatccg cctcatcgcg tccggtggca tgagcagcat cgctgatctg
     3421 aagaaattac aggccatggg cgtctacggt gccatcaccg gcaaggcgct ctacgagggg
     3481 aaaatcacga tggccgaaat ccgcgcatta gggggaatgt aagatgttgg caaaacgtat
     3541 tgtaccgtgc ctggacgtcc gcgatggccg cgtcgtcaaa ggcaagcagt ttaaacatat
     3601 ccaggacgtc gacgatcccg tcaaattggg gaaattctat tccgaccagc tcgccgatga
     3661 actcgtcttc tacgacatca cggcgaccca tgaaaagcgc catatcttca tcgacgtcgt
     3721 ccgcgccgta gccgaagcca ttaccattcc ctttaccatc ggcggcggca tcagcagcat
     3781 cgacgacttc caccaggtcc tgcgggccgg tgccgataag gtatcggtca attcggctgc
     3841 cgtccgccgt cccgaactga tacgcgaagc ggcccagcgc ttcggcagcc agtgcgtcgt
     3901 cctgtccatc gatgccaagc gcgataacca cggcagctgg caggtctacg tcgaaggggg
     3961 ccgcaagaat acgggcatcg acgccatcgc ctgggccaaa cagggcgtcg ccctcggtgc
     4021 cggcgaaatc tgcatgaatt ccatggacgc ggacggcgaa aaggccggct tcgacctgga
     4081 actgaaccgc cgcctggcga cggaactgcc tgtgcccatc atcgcttccg gcggtgccgg
     4141 cacgatgcag gatttcaagg atgtcttcga cgtcggcgtc gatgccgccc tggccgcttc
     4201 ggtcttccac ttcggccaga tcgacatccc cgatttgaag acctatttac agggacaggg
     4261 aattcccatg agaggggcag aaaaataaat ggaaagtgta tcgatgaaag acattaaatt
     4321 tgatgaaaag ggcctcgtgc cggccgtcgt ccaggaagga tgcagcggca aggtcctcat
     4381 ggtggcctat atgaacgccg aagccctgaa gcgcaccgtc gaaagtggcg atgcctggta
     4441 ttacagccgc agccgccagg aattgtggca taaaggcgaa acgtcgggac atttccagca
     4501 catccgctcc atcgccgtcg actgcgatgc cgacacgatc ctcctgaccg tcgaccaggt
     4561 cggcgccgcc tgccatacag gccacaaatc gtgtttcttc cggtccatga cggaatggga
     4621 aaagacctat acgggcaact tgtccatgct gtcggacctg cgcgaagaaa tcaaggaaaa
     4681 acgcatccat ccgacagaaa aatcctacac ggcttacctc ctgcagacgg gcatcgacaa
     4741 gatctgcaag aaaatcggcg aagaatcgtc ggaagtcatc atcgccgcca agaacgccga
     4801 ccccgtcgtc aaggatacgt cggaagccgc cgacaacctg cgcctggaag tctgcaagga
     4861 atcggccgac ctcttgtatc acctgtccgt cctctgggag gacacgggcg tcaccgtcaa
     4921 cgatgtcatg aaggtcctgg aagaacggtc ccatgtcaag ggcaataaga agactgtcgg
     4981 ccatttggac aagacatttt aagcaagagg aggatggcgc gatgaagaaa gtgatttttg
     5041 acgtcgatgg cgtcttgctc agtgaaaaac gctatttcga cgtatcggcc ctggtagtct
     5101 gggaatggta ttacagcccg cgctacatgc acctcaaacc ggaacggatc acggccgacc
     5161 ttgacgatga taccatcgcc gccctgcggg cccgcttctg gaggaacgac gatatcttat
     5221 tgtggctcaa gaaacacggc gtcaacagca actgggacat ggtccacgcc cacatcgtcg
     5281 tcaccttgtg gctcatgctg gaaaagtatg tcgccgacca cggcggtctg gaagggagcc
     5341 tgaagaaaat cgacgatatc cagtacctgg gctctctcct gcggggctat cccgtaccga
     5401 cggccgacga cgtctatcgc cgcctgagca atgtcatccc cgaacgggcc ggcaaggacg
     5461 aagtctttac ctatctggcc aatgccgtcc agtcgtccct gggaccgggc tccaagcagt
     5521 ggacgcccct cgattcgccc ttgtggcagc tggaattcga ggccttccag gactggtatt
     5581 tcggcgacga actctatgaa cagacgtatg ggaaaaagcc ttatgcaccg gggaaaccgg
     5641 gctttttgac gcgggaagaa ccactgggga ctgttgacgg catccgcaac atgttccggg
     5701 aattgaagaa acggggctat cagatcgcca tcgccacggg ccggtcgaaa atggaaatgg
     5761 agattccctt ccggacctat ggctggctcg acgaattcga cgaacactac gttgcgacct
     5821 acagcgacgt cgaagaagcc gaagccatgc tgcacatgtc cctggacaag cccaacccct
     5881 tcgcctatta cctcggggct ttcggcaaga ataagaagca ttacctcgac tacgtcagcc
     5941 atcccgacga cttcaagaag ggcatctact acgtcgttgg cgactccctg gccgacgtct
     6001 ggtgcgcccg ggccatggga gccaccatga tcgggaccct gaccggcatc gacgggccgg
     6061 cggccaagcc catgttccag aaggagcacg tccgctacat cgtcgattca gtcgaagaga
     6121 ttttagatat tttaccgtag gaattatcat gaagattaca gctattgaaa tcgggaagat
     6181 cgccattccc ttgaagacgc cgttcatcac ggccctgcgc cgcgtcgacg tcgccgaaga
     6241 cctggtcgtc aaggtccata ccgacgacgg ccacacgggc tacggcaacg caccggcgac
     6301 ggtcgtcatc accggtgaca gtcaccaatc ggtcgccgct gccattaaat ataccatcgg
     6361 gccgaaactc atcggccggg atatcgacga ccgcgaagat atcctcgaca cgatccatca
     6421 atctatggtc cacaacacgt cggccaaggc cgccctggac atggccgtcc acgacctctt
     6481 cggccagcgt tacggcctgc ccttgtaccg cttcttcggc ggccgcaaga cgcgcatcgc
     6541 ctcggacctc accatcagcg tcaacgcgcc ggacacgatg gcttccgacg ccgtccaggc
     6601 cgtttctcgc ggctacagcc acttgaaact gaaagtcggc atcgacccgg ccatggactt
     6661 ccagcgcgtc gccgccatcc gcaaggccgt cgggccggac gtcactatcc gcctcgacgc
     6721 caaccagggc tggaagccga aagaagccat ccgcctcatc ggccgcatgg aagacgccgg
     6781 cctcgctatc gaactggtcg aacagcccgt ggcagccgct gacttcgacg gcctgaaaca
     6841 ggtcacggac cacgtcgcga cggacatcat ggccgacgaa tcggccttca gcccgcgcga
     6901 cgtcttccgc ctcctggcca tgcgggcctg tgacctcatc aacatcaaac tcatgacggc
     6961 cggcggcctg gccccggctg cccagatcgc cgccatggcc gacgccgccg gcgtcgcctg
     7021 catgatgggc tgtatgctgg aaagcaaagt cggcatcacc gccgccgcgg ccctgtctgc
     7081 cggcaaggcc gtcatcacca agaacgacct cgacgccgcc gacctcatgg ccgccgaccc
     7141 cgtccggggc ggcatcacct atgacaaaga ccacctcgtc gtccccgacg caccgggcct
     7201 gggcattacg gccattgatg gctggctgcc attaaaagat tgacaccggc gccgacattt
     7261 tttataatta aataagtctg aaaaagttag tcatggaacc cttcggcttg tacagcaggt
     7321 ctgaagggtt cttgtacgat gaatcgtttt ttacatagga ggtataatgc gtttacgaag
     7381 aaaaccgtgg attgacgagg ctatcaagga ttatagtgat tttctccatc tggaaagctg
     7441 cgaaagctat aagggaaaat ggcgttccct ctttaagaat cccgatgcgc cgctgtgggt
     7501 cgaattgggg acggggaagg ggaatttcat ttcccagatg gcccagatcc acaaggatgt
     7561 caatttcatc ggcatcgaga tccaggtcgg cgtcctctat tatgccggca agaagtgcgc
     7621 cgctgacgaa gtggacaatg tcaagcttct gcgctttgac gtagctcgcc tggaagatat
     7681 ttttgcccca
//
LOCUS       DAWCYS010000056         7604 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_612_length_7604_cov_8.659293, whole genome shotgun sequence.
ACCESSION   DAWCYS010000056 DAWCYS010000000
VERSION     DAWCYS010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_612_length_7604_cov_8.659293"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1689 a         2203 c         1947 g         1765 t
ORIGIN      
        1 ataagtaggt gagaccatgg aagccgctta ttatgaaatc gacccgtccg gccaggcgcg
       61 ctgccgtctc tgtccccatc actgccacat cgcccagggc catcgcggcc gctgccagac
      121 ccgctattac gatggcagga acctccaggc cctgaactac ggccaatgca cggccatcgc
      181 cctggaccct atcgaaaaga agcccctcta tcgcttccat cccggttctg ccatcttgtc
      241 cctgggttcg tggggctgca atttcacctg ccccttctgt cagaactggc agatttccca
      301 gcaggaagcg ccctttcggg aactgacacc ggagcaggcc ctggccctgg cccggcatta
      361 tagggccgac ggcagcatcg gtctggccta cacctacaac gaaccgacct tgagctatga
      421 attcatcatc cagacggccc gcctcgtcca caaagccggc ctggtaaacg tcatggtcag
      481 caatggctat atcgaaaaag aaccactcca ggccctgctt ccctatatcg acgcctggaa
      541 catcgacctc aagggatttt cccaggactt ctaccagtcc ctctgcggag catctctcca
      601 gcctgtgctg gacacgatcg aagcggccgc tgccgtaagc catgtcgaag tgacgacgct
      661 ggtcattccc ggcgaaaacg atgacccggc ccagatggac gccgaagccc gctggctggc
      721 atccatttta ccggacatcc ccctgcatct gacccgctat ttcccgcagt accactggtc
      781 tgccccggtg acacctgtag aaacgctgaa gaagctgcag gccgcggcca ttccttatct
      841 tcactacgtc tatctaggca atgtctagct gatgctatcc cttgtacgtt aaacgattat
      901 cgtaaagttt aaacttgcgc actgttttga acatgatata ataagttaca gtttattttt
      961 aatttttaaa aagaagtggt ggtaaagaat ggcattaaag gaatttactt ttaatattgg
     1021 tgaaactacg cagactgtca agctcccgga agaacacatt gtcggggtca tggaagggaa
     1081 acacgtccct gccgtcgacg tcaaacaggc gacgatcgac tgcatgcgtc atcctatcgg
     1141 cagcgccccg ttgcaggaaa aagtccagaa aggcgataag gtctgcctcg tcgtcgccga
     1201 cgtcacccgc acgtggaacc attccaatca attccttatc tatatcatcg acgaactgaa
     1261 cctggccggc atccccgatg acgacatcta catcgtcttt gcccagggct cgcaccgcgc
     1321 ccagacgccg gaagaagacg tcctcgtctg cggcgaagaa gtcgtccgcc gcatcaagac
     1381 ctatcagcac gactgcatgg atccgactct cgtcgacttc ggtacgacca agctcggcac
     1441 accattgaaa ctcaataaac acgtcgtcga cgccgacaaa gttatcatcg tcgacggcat
     1501 tacgacccat ctctttgccg gttacggcgg cggccgcaag ctcatcctgc cgggcgtctc
     1561 cggttttgaa acgatccagc gcaaccattg ccacgcttta gctgacgaat tcggccacgg
     1621 catcaatccc aagacgcggg ccctcaagat cgacggcaac ccgctcaacg atgacatggt
     1681 cgaagcctgc agcaagatca atccgtgctt cctcgtccat tccgtcatca acggcgacgg
     1741 tgaaatttgc cgcatggtcg gcggcgactg gtacgaagca tggcgggccg gcacggaagc
     1801 cgtcctcgac atccagcgcg tcccgatgaa agaaaaatcg gacgtcgtca tcgcctgcgc
     1861 cggcggttat cccagcgacg tcagcctcta ccagggctgc aaatgctacg atccggctga
     1921 aatggctgtc aaagacggcg gcatcatcat cgccatcatg gaagcccggg acatcaaaga
     1981 accggccatc tacatggaca gcttcaaata cgacacgatg gaagacatgg aaaaagccct
     2041 gcgggcacac ttcactatcc ccttcttcgt cgccttcaac ctcttctgcc tgactcataa
     2101 ggacacggtc atcctggtca cactgccgcg taacttcaac gacatccgcc gcactggcca
     2161 gattccggtc gaaaccgtcc aggaagcatg ggacctggca cagcagaaac tgaaagaaca
     2221 gggcaaagac aaggactacg ccatcaacat catgccccat gccaccaaaa tcatgccgat
     2281 tttacaggac aaataacctc attattggtc ccgtagggga cgtcctttgg gcgtcccgcc
     2341 taattccata acccgtaggg ggcgcacgaa ggcttttacc atcatgaaag gcccctttct
     2401 cctgaaagtt tgtagtacag agtttgtaga agagggcttt aaatttaaaa gccttcacta
     2461 aaaactacaa acttaatact caaaaaggcg ctgtctttat gcgacagcgc cttttgatgt
     2521 cctatcccaa gtctctcctc ataggagagc tgtcagcgca gctgactgcg agattgcttt
     2581 ttgaatcacc gtgcaggtta cgagattttc acaagcgggt tcgaaaatgc aacctctccg
     2641 gcctttcagg ccacctcccc tatcagggga ggcctgcgga ctgcgacctg cgacctgcgg
     2701 accgcgcccc ccattattcc caaaggctat acgcatctat tatttaatgc gcaaataaca
     2761 taatatattt tcgtatacct catgacaagc gtaaaatcct atgctataat atagccaaga
     2821 gatgcgaaag gaatcgcaag taagttgctt cctttgtaag cgcattagaa aggggggagt
     2881 ccagtggaaa tagagaaagt agaacatgcc gccggcggta aggggtacat cttgaagaga
     2941 aaacttctga gcgccaccga gctgaaagac aaggtaggta tgttcgccga aatcacattg
     3001 aaacctggat gttccttagg ctatcacgtt cacagtgaca acgcagaagc atattacatc
     3061 ctttccggag agggagaata tgacgacaac ggagaaatcc ggaccgttaa accaggagac
     3121 gttacttaca catcagatgg taagggtcac agcttggaaa acaaaagtca gaacgattta
     3181 gttttcattg cgttgatcat taacaactaa ataatgtcca taggatcagc aagcgttcgt
     3241 cgatgaacat tacgaattaa cttatcttga ttgaagtgct tcgaactttt cgaatgggtg
     3301 tgatgttttt caaataggtt agtctagtaa atccaagcgc aacgaacgat tattgtttca
     3361 acatttttgt acgaggttaa agtaaccaga accaaaaacg agatgaatat gaatcctata
     3421 acgtagagga ccggtagaca tatgtcgcgt gtgtatcggt ccttttacgt gtttttttta
     3481 cctgacttgt cccttctcca ttgacagaaa ccgtcccctc ttctataata tactaaaacg
     3541 gtattctata gaattcgttt agatttatca aagggggaga caattatgtc caacgaatct
     3601 caagaccggg tccaagaaca agcccgggaa gccatccgcg gcagccaggg ccagcatcgc
     3661 ggccgtcaat tcgccttgtg gatgggcgcc ttgatcatcg gcgccatctt aggctggctc
     3721 ggcattccgg cactgaacca attatttgat ttcattgcaa cagttttcac gcgcttattc
     3781 cagttcattg ccgtaccgac tatcgcctta gccgtcatta ctactttatc ggcgctggga
     3841 gccaaaaaag aaacgggccg catcttcgcc catgccgtca cgtacacgct cttgacgacc
     3901 atctgtgccg ccgccatcgg cctggcactc tacctctgga ttgccccggg caacctgccg
     3961 gtagacctca tcaattccgg cgtttccaat gtcccgcaga aattggaaaa agtcacctat
     4021 tacgaccact tcctgtcggt catcccgaat aacatgctcc agccgttctt gtcgggcaac
     4081 gtcctctcgg tcatgctcat cgctgctgct gtcggcctgg ccctggcctt catgcctaag
     4141 acagaaaacc gcgccgtcct cctgaaaggc ctcctgggct tacaggaagt cctgttcacc
     4201 ctcatccgtg ccctgctctg gattttgcct gtcggcatcc tggctttctc ggcacagctg
     4261 tcggctcaga ttgaagccgg ggtcatcgtc ggtgccctgg gtaaatatac tctggtcatc
     4321 ctcggcggca acctcatcca gttcttcatc gtcctgccga ttttcctggt cctccgcggc
     4381 ctcaatccga tttacgtctt ccgcaagatg gctccggcca ttgccgtcgc cctgttcacc
     4441 aagagctcgg ccggtaccct gccggtcacc ctggcttcgg ctgaacagcg cctcaacgtc
     4501 catccccagg tatcgcgctt cgtcctgccg atctgcacga ccatcaacat gaacggctgt
     4561 gccgccttca tcctggtcac atccctgttc ctcatgcaga acggcggctt cgacctctcc
     4621 atgggcacca tgattgcctg gctcttcgtc gccgtcctgg ctgccatcgg caacgccggc
     4681 gtccccatgg gctgctactt cctgactttg tcgctcatgt ccggcatcgg tgcgcctatc
     4741 ggcatcatgg gtatcatcct gccgatctac accatcatcg acatgatcga aacagccgaa
     4801 aacgtctggt ccgactcggc cgtctgtgcc atgaccgacc acgacttagc ttctgaactc
     4861 gatgcccagc cggcaccggc tcagcattaa gagcgtaaaa aaaccgctgt cttaaagacg
     4921 gcggtttttt ttagttgtta ggggctagca taactgttag ccgcgaatgg tagcttcttt
     4981 catggctttg acgtagtcat agatgtgcgg agccgcatcg tcgccgtatt tagcgatgat
     5041 tttgacaatg gcactgccga cgatgacgcc gtcagcgacg cggccgattt tttcggcctg
     5101 ttccgtcgtg ttgatgccga aaccgacggc acacggtacg tctgtgactt ctttagccgt
     5161 atcgatgatt tccttgaggt ccgtcttgat ttccgtccgc gtccccgtga cgcccaggga
     5221 ggaaacgatg tacaggaagc cttcgccgac tttagcgatt ttctggatac ggtctttcga
     5281 cgtcggcgcg atcatggaga tgatgtcgat gccatgggcc ttagcggcag cggcacattc
     5341 atcgcgttct tcaaagggca tatcggggat gatgatgccg tccatgccgg tttcctggca
     5401 gcgggcaaag aatttttcag cgccgtaatg gaagacggga ttgagatagg tcaaaaagac
     5461 caggggaatc tgcgtttcct tgcggacccg ttcgaccagg gcaaagacgt cgtcaatgcg
     5521 cgtccctgct ttcaaagccc gcaggtccgc ttcctggatg acgatgcctt cggcgatggg
     5581 atcggaaaaa gggatgccga tttcgatgag gtccgctccg gccttttcca tggtcatgat
     5641 atattcatac gtcttgtcca gcgacgggtc gccggctgtc aagaatccga taaaagcttt
     5701 gccgttctta aaggcgttgc caatcttact catgtaagtc cctcccttta tatcttgcaa
     5761 tggcggcgac gtctttatca ccgcggccgg acaagcagac gatgatgatg tcgtccttgc
     5821 tcatcttcgg tgctatctgc atggcttcgt agacggcgtg ggcgctttcg atagcgcaga
     5881 tgatgccttc cgtccgggcc aggtattcaa aggcttcgac ggcttcgtcg tccttgaccg
     5941 ggatgtactg agcacggccg gtctgatgga gataagcgtg ttccggcccg atgcccggat
     6001 agtcgaggcc agccgaaatg gaatagactt cgtcaatctg accgtcttca ttctggcaga
     6061 aaatggactt catgccgtgg aaaatgccga cttccccttt ggccatagta gcggcatgca
     6121 tcttggtgtc gatgcccttg ccagcagctt cgcagccgat gagcttgacg tctttatcgc
     6181 cgatgaagtg gtagaacatc cccatggcat tgctgccgcc gccgacacag gccaggacag
     6241 ccgagggcag tttcccttcc cgttcgagga tctgctgacg cgcttccttg ctgatgacgg
     6301 actggaagtc gcggaccatc atcgggaagg gatgaggacc catggtcgag ccgatgacgt
     6361 agtgcgtatc gtcgacgcgg ctgacccatt cgcgcatggc ttcgttgacg gcgtccttga
     6421 ggacccgcga gcccgacgtg acggcgtgga ccttggcgcc caggagttcc atacggtaga
     6481 cgttcagggc ctggcggatc gtatcttctt cgcccatgaa tacttcacat tccaggccta
     6541 agaaagccgc tgccgtcgcc gtagcgacgc cgtgctggcc ggcgccggtt tcggcgatga
     6601 cccgcgtctt gcccatcttc ttggccagca ggcactggcc cagggcgttg ttgatcttat
     6661 gggcgcctgt atggttcagg tcttcgcgct tcaagtaaac cttggcgccg cccaaatctt
     6721 tggtcatccg ttcggcatag tagagcaggg acggacggtt ggcataattg tcaagcaagt
     6781 ctttcaattc agcctggaaa tcgggatcac tcttataatg gctgtacgct ttttccaagc
     6841 gctggatttc attcatcaat gtttccggta tgtattgacc gccgaagggg ccaaatcttc
     6901 ctgtactcat gtagttacct cctgtaagca cgtacttgtg ccatgaatcg tttcattttt
     6961 tctttgtctt tcacgccgtc tgtctcaatg ccgctgctca catccagggc aaagggttgg
     7021 agcgctgccg cctgggcggc attgtcggga ttgaggccgc cggccagaaa gtacggtctg
     7081 tctatatggg ccagcagggc ccagtcgaaa cagtccccgg tcccgccggc gccgtggtcg
     7141 agcaggatgt agtcggcccg gctacaccgg gcctgccgga catcggcttc gctggcgatg
     7201 gaaaaggctt tgatgacccg taccttcaag gtctgctgga cagcttcgat catcgcgtcg
     7261 tcttcgcatc cgtggagctg gacgatctgg atggtcccgg ccgtcaccag ctgccctatg
     7321 tgtttcagac tgtcatcgac gaagacgccg acggccggga tagacgggtc cagggccttg
     7381 cgcagggctg cggcctggtc gtcgctgacg cggcgcttct tgccggcaaa gacgaaaccg
     7441 gcataatcgg gccggagttc attggcccag gcaatatcac agggccgctt gaggccgcag
     7501 aacttgacct taaccacgat agcctccggc cagctcgtcg agcatggctt tcttatcggg
     7561 actgcgcatg aaggtttcgc cgatgaggac ggcgtcgacg ccgt
//
LOCUS       DAWCYS010000057         7560 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_616_length_7560_cov_5.224650, whole genome shotgun sequence.
ACCESSION   DAWCYS010000057 DAWCYS010000000
VERSION     DAWCYS010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_616_length_7560_cov_5.224650"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2092 a         1693 c         1883 g         1892 t
ORIGIN      
        1 aacgcggatg ccatcaaagc ggctctgaca aaatatttca agtccagcgt gttcggcaga
       61 aactacacta cgcagaaggc aactgacacg gtgaccatct cttatgccca gatcggccgt
      121 atcatcctgg ataactctga tacgaccggg gtaaacgatt atgatgattt gacgattaat
      181 gacggtacag ataacatcat ctgtgcggtg gataatcttc ctgtagttgg ggcggtgacg
      241 ctgacatgag tgatcataat tggatgcggc aaagcactgt agatatcctg caatacctgc
      301 cgacgtttct ggctcacagc cagcagttcc aggccgcgaa tgatgcggac agcaaagaac
      361 atgataccat ccggattgat ttgcaggatg tgttggacca gttctacgtg aagtcagcca
      421 cctggggcct tgagcgctgg gaagaactgt gcggtatcac tacggacaaa acgctcagcg
      481 acagtgtgcg tcgcagcacg ataatcgcca agcttcaaaa tccgggcagc gtgacggaag
      541 tcttcctgac gaacctgatt aacggataca ttgcggacca gcagggatac atcatcagct
      601 atcccagtga gtaccggatt gaagtgcttt atcatggtgg ccagattacg gactatgaaa
      661 agttacgtac tgccatcagc acatacatac ctgctcacat cgggtataag ctggtgacca
      721 tcaccaaggc agaccttgaa tatcatggag ccggaactgt gcagtgttac cggaaaaatc
      781 ttgttgatat gtctgtaaag tacagcatca atgttgatga ttcaccacac tatattgctg
      841 gcgcagtcgt tcataattac aagctcatta aaatttcggg gggcaataga ccatggctaa
      901 atttgcaaat ctaaccttta catctcaggg cacgcaaatg cttgtccagg ctcaaaattc
      961 tcataggtta acttttactt gcgggaaact tggttccggt gttcttgcag attcggatga
     1021 tatctccaaa ttcacggatt tgaaatgtcc taaaatgaca ctgccgattg tcagcgtgga
     1081 tgacagcaac gaagaaaagc tggtgcttac gttcgacacg tccaacacgg aactggaaga
     1141 aggatttgtt tccagggaaa tcggcatctt tgctaaactc gataacggtg cagaaacact
     1201 ctatgcatac tccaatgccg gcaacaacta cgattacatt ccgagcaagg atactccatc
     1261 cgatgaaaat cgtctggtcg tcaatctggt agtcagctcg tctgccaata tctctgtgca
     1321 gattgacaaa tctatcgtct acacgcataa gagcgatgtg gaagagatga ttgccactca
     1381 caacgcttcc gacacggctc atgaaacccg gttcaagttg tttgagaaga ttgctgattt
     1441 tggcaatgac atcatcaaga agatggccat cactacggcc atcacagcca ttacagcgct
     1501 tgaaacgaat tcgtggtttg ggcagctcct caagatggta ctgacggcat ccggagtgaa
     1561 gtacaatatc gcacagaatg ggtatgtctg ccttggaagc ttctttggcg gcctaattat
     1621 acagtgggga attgcgactg gtagtacggc agaagcacat gtaacatcag cctttaatgt
     1681 tacattacca ataaactacc aaaaacaaat tgcacaagtt ttggttaaca ctgcgaacgg
     1741 gggtactaac aacacatggg aaagcacaac tgtatgggcg caaggagaaa cggtaaaatc
     1801 ttttgttggg tacatggttg cgggacaccc atttacgact accccaaaga ttagatgggt
     1861 gacattagga aaataataca tttagtcgca tatagctatc cagtgccccc aaagctgcgt
     1921 tgttgataaa tcgtttcttc tgcctgttac tttgaactta ccattagtat aatcaagcgt
     1981 ggacatacaa agtgaattat tatcactgtt catatcaacg actataacct gaagcggatt
     2041 ggcggataga ggcatattaa caaaagttcc actatcaaca aaaccaaaat ttccccactg
     2101 tctattaatt tgtgccaatt gcaaaccaga agacatctac aggcaagccc gcatcatgca
     2161 atctagagct atcaacatcg acaatgattc tcatgctcga accaagtggg gttgcataca
     2221 tcaacggata cggttccgaa cgagcgttgg tttcaaaaga ggcaacaggg ataaccacta
     2281 aaatcgtaca gtcaatatta ggctttatca catttaaaga ctctactttt gtggttaact
     2341 ttccccactg tatcaaattc ccattgctat tatgttcttc ggctttgcta ttttgccagc
     2401 ttgactttca gatgcgctcc acgctgttac agtgcttagg tcactatcat ttaatacgga
     2461 cacattttca taaacccatt cactagtagc gcaggtgccc aaaactacaa atttaatttt
     2521 actgaaagcg atgggtagtg tgattacctc atctgtagtc gcttttaccc actgtatact
     2581 ggttgttttc ggaagagaga actatggaga atattcacgg tccctgaaaa gattttcttt
     2641 tattgcaatc cagcatttac gctggttgtg gacatttctg ggaatccgta attctggaat
     2701 caccgtattt tcatcagctt tgctggaaac agtagtaaca accacgaaat cacaaaatat
     2761 atagtgaaat cgtggttgcc atctgcttta cagtatcgta ttagtaacat tattttaata
     2821 gttcaatgca tttccggagc tgcctaatat ttttgtgggt ataaacgcgt tcggtgacat
     2881 cgccacctgc gtgccctaag atgcgccgct tagctgtttc gttcgctcca gcattgtcaa
     2941 gcagggttgt gacagtatgc ctgcaatcgt gtgttgtatg gccggcagcg ttgatgagct
     3001 gcatgactga gcgccagatg atgcaatagc gactgtagtc atatgggttc ccgttggtgt
     3061 cactcagaag agtagttccg ggagaatcca tcctggcaat tatcaatgga aggattctgg
     3121 gatggatggg aataacccgg ataccggaaa cggttttgct cttggtgatc cggatgaaac
     3181 gctgcctgat gttgacgtct gacttatgaa gatggagtag ttctccgact cgcatacccg
     3241 tatagagaag tatcagtaca gtatcaacgt tgtcattgca agcggctttc cataatcggt
     3301 ttatcttctg ccggctaaac ggcttgtgtg gccggacctg cttgttcttt ccgatggata
     3361 agagaggagc atagtttttg ttgctcagct cgattttgct ggaatactga gagagcaatg
     3421 aaatgagtga tcgcaccttt ttaagcgagc tgtacgacag cccgcttttt ctcatgccat
     3481 ctatgattct ttgatagtcc atgtatttca gactggaaaa aggttcctgg tgcagagatg
     3541 agagatgctt aaatgaattt tcatagctgc acagtgttga ttttgatggc gcggtatctg
     3601 ccgtgtgcgc tggtagccac cgatggtaca gttcttctaa tgtgatttga tgccccggga
     3661 gggggtgatg gttatgagtc ttgttgtagt cagcttgaaa aatttccgcg tcaatctggt
     3721 tggtaaaata ttcaacaggt ctttgcttac cattctccga tattacaaaa ataaacggcc
     3781 gcctccggtt tccggacagc cgtttgatgc agccgtatcc atttggttta cgcataataa
     3841 aaaacctcct ttacggaggc cattttacta taggaggatt aattaatgaa cggtacgaac
     3901 gttgattact acgtggcggg gttcgatgca gatggcaagc gcgttggttc gctgatctgc
     3961 gactttgacc ctggcaagga caagaataca ggaaagctgg cttctttaac ggaaaaggct
     4021 aaagatttgt tcgaggatgc agctgtcgta gatgtcattt ccgcagcaga ttataaccag
     4081 tacctgactg gcgaatatgt acgaggtgca gatggtaatc cgaccgcata cattgcaccg
     4141 gagccaacag ccgaggagca gaaagcaaaa aaacaggcag agttacagtc tacgtatgaa
     4201 tctgacaaag aagaactcat gaagtattat ctggctgcgg ctcttgccgg tgatgcggat
     4261 actcagaacg aactcagaac tgaactgaca aatctcgatg cagaggttga ttcggatatg
     4321 aaagcattga acgactaaca ggaggacccc aatactggca tttaaactcc agaagagatg
     4381 tgttcgttgc ttgaaggttt tacgtgatga cggtacctgc cagaatccga agtgctgcct
     4441 ttaccaggaa gaagcgccca aggatgctga accgggcaag accgccgaca gcaacagcac
     4501 aactaaggag gctaaatagt ggattggtgg cagattcttt tagcaggcgt gccatcctta
     4561 ttttcatgca tcctgttgta taagtggaaa cagcagcgaa aacgaattaa agagcaagaa
     4621 gacgaaaagg aagtacgaaa tgaagcattg gtcaagggag tgcaggccct gctgcgcgac
     4681 cgtcttatca ctggaatgga gacatgcctt gaaaaaggat atgccccgat taatacggtg
     4741 gaaatcatgg gctccatgta tgccgcatac tgtgaccttg gagggaacgg tattgtaagc
     4801 ggaatctata agcgctttag cagccttccg cacaagcttc cggatgacgt gtaaggcggt
     4861 gattgagttg ttccagtttg aaaaaattga tatcgaaaac attctagtca ttattgctct
     4921 ttcagcgagc ctgataatgg ctatttttta tgggctcgat aatttggcca tgagcattgt
     4981 gaccggcctt ttgggataca tcggcggcac aatcaagggc tccacaccga aaggaggtga
     5041 caataaatga acgtatttat taatcctggc catgcaccca atggcaatcc ggacccgggc
     5101 gcggtgaacg gcaatactgg gctgcgtgag tgcgatgtag cactggcagt gggtgacctt
     5161 gttgctcact atctcaaggc tgctggtgta ggtgtgacga acgtcttcca gtgcgacagc
     5221 ctggaagaaa tctgtgatca ggcgaatgcc agtggtgcag atctttttat ctctatccac
     5281 tgcaattccg cagaagcagc ttccgcaacc ggtacagaaa catgggcatg tgcgggcagc
     5341 tctgccgggc atgatctggc aaactgcata cagaatcaga ttgttgacgc attgggtact
     5401 atagaccgtg gcgttaaaac cgctacgcct ggcatcaatg gcctttacgt actgactaac
     5461 actgatgcac cagcagttct tattgagctg gcctttatct ccaatgataa agatgaacga
     5521 ctgctggcgg aaaagaagga cgactttgcc agggcaattg cccgaggtgt aacggactat
     5581 gaatgccagt aacctaatac caagaaagta attgaaaagg agaaaattat tatgagcaaa
     5641 tggaccgatg taagagacaa tattgttgat gcgctgaatg tcgatgtcgt gaccgatgaa
     5701 ctgaaagacc aggtcaccaa cactatgttg gaacaagtta tgccaatcat tgaaaatgcc
     5761 gtagatggct tttctgaaaa ggtgaaggca caggcccctc aggaatccgg ctggtgccgc
     5821 gtccgtgatg gcatcgttct gccgctggtc atggaagggc tggtgtttgt ggtaaagacc
     5881 gtgctgacga aagcaacggc ggagaatgca aaagaagaga ccgccaaggg tgtataaaac
     5941 cgaagattga tgatataata aagggcatag ctgtgacttg tgctatgccc ttatttttat
     6001 gcgacttccc gccggatccg gactgtaaaa aagtcctgtg ataccagtac ggtgaggttc
     6061 gtatgtggta tgggtaagtt caccatagga aaaagctcac gcattttgcg tgagcttttt
     6121 tatagtttgt agtacggagt ttgtagaaga tggctttaaa tttaaaaacc ttcactacaa
     6181 actacaaact gcaaacttaa tactccaaaa gattctgtcg cacgaagaca gcctctattg
     6241 cctactttag gcacggccct atccatgcta taatagagac atcgcaaatt gtgaaaagga
     6301 agtgtaagct atgacagtac agctcgaaga attattgaaa gcctttgaac tgaataatcc
     6361 ctatctcaag ttctatttaa ataaaaatga cggccatatg gccttggtca cggaaatccc
     6421 cggtgacgac aaggcggaaa atgaagtcac agccaatccc gatgcgtata tcaagctgcc
     6481 gacccaggcc gacctggatc tgccggaaat gatcaagggc tttgtcccca tgatgaaaga
     6541 cccgaagcag cgggcggctt ttcagcagtc catcgaagcg ggcaagaccg tttcccagct
     6601 ggaacgggaa ttgaaggaca tgggcctggt ccagttctgg tatacctttc agcgcatgga
     6661 attccgcaaa attgccaaaa agtggtgtga agacaaccac attgattatg aagaataggg
     6721 gagctcggag atgacgaaga tttcagatat cggtgaattc ggattcatcg atgctattaa
     6781 agatcatacc ctcttccatc cggaaaacgt cgccgtcggc atcggcgatg acggtgctgt
     6841 ctacacgacg acgccgggct ggcagcaggt agctgtcatc gacacgatgg tccagaacag
     6901 tcatttcatc atcggtcaga cggcgacctg gcatgacgtc ggcttcaagg ccgtagcctc
     6961 gaacctcagc gacatcgccg ccatgggggc cgtgccgacc catatcgtcc tgtcgacagc
     7021 cctggcaccg cagatggacg tcgatgacgt cattgacatg taccgcggca tcaaggatat
     7081 ttgccgcacg tatgaagtca atatcctcgg cggcgatacg gtcatgtcca aagaaggcgt
     7141 cgtcatcact gtcgctgcct ttggcgaaat cgaagccgga aaggccctgc tgcgcagcgg
     7201 agcccaggcc ggcgacgtca tcgccgtatc ccatacgatc ggcgattcgg caggcggcct
     7261 cgacgtcctc ctggccggac aggacggata tgaacatttg aagaaagccc accagtatcc
     7321 ggaaccgcag atttccctgg gacgcttact ggtacagcat cattgccaca gcctgaacga
     7381 catcagcgac ggcctggcta gtgaatccaa tgaaattgcc aaggccagcg gcgtcgaact
     7441 ggtcatcgac cgggaccagg ttccgacgag cgctgagcta aaagcctggg ctgcggcatc
     7501 gggcaaggat atttggaact tcgtctgtaa cgtcggcgaa gactatgaac tggtctttac
//
LOCUS       DAWCYS010000058         7493 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_622_length_7493_cov_8.812315, whole genome shotgun sequence.
ACCESSION   DAWCYS010000058 DAWCYS010000000
VERSION     DAWCYS010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_7493_cov_8.812315"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1715 a         1886 c         1792 g         2100 t
ORIGIN      
        1 gatggcatcg agccctcaga aaaacctgca attcatctca ccgcctaaag aggcgagaga
       61 cttcttgcag aatcaagtta aatccacgtc aatctctcct tttcttagaa acacggccac
      121 accccattca atcctaatga cgggccaaaa aggcttacca ggccgtcccc tacattatca
      181 catataggcg ataaaaataa tacaccgttc acggccagtc gcaaaaaaaa cacggcctgc
      241 ggccgtatgc gtaggggctg tcccgataag ggcagcccgc tgtgagattt ctccacaacc
      301 cagtctggca tagtggcagg cttttacgga ctacgaatca cgagcaacga accacctatc
      361 atggatccgg ctatgtacag aattcatgcg ggctcgccac gtgcgagccc ctacgatgcg
      421 gctgcgccgc tacaggctta tgacctgatt acaaccataa gaacccctca gaccgccttt
      481 ggcggccagc tccccttcac atgggagcct ctggcgctcc tgcgccttat atttcagcgt
      541 ttattgcggc aaagccactt ttgagtccgg gcaacaaaaa aagacctgtg tacgtaatcg
      601 tacacaggtc cgataatttc ccttgtgaaa ttatttgagt tcgacagtag cgccagcttc
      661 ttcgagttta gctttgagag cttcagcgtc agctttagca acgccttctt taacagcttt
      721 cggagcgccg tcaacgagag ctttagcgtc tttcaagccg aggcccgtaa cttcacggac
      781 aactttgata acgccgattt ttttgtcgcc agcggactgg agaatgacgt cgaattcgga
      841 tttttcttca gcagcagcac cgccagcagc cggagcagca gcaactgcaa ccggagcagc
      901 agcagaaacg ccgaatttgt cttccatagc ttttacgagt tcggaaagtt cgagaacagt
      961 catattttca atggcttcca tgatttcttc tttagtcatt ttgaaatacc tccatagtat
     1021 caatttttga gtttaatttt taagttatgt aagctaaatt aagcagtttc ctgttctgct
     1081 tttttagcgc gtacagcgtc aagcacataa accatgttgc ggatattgcc ctggagtacg
     1141 ttgacaaggc cagagatagg agcctgcatg ctgccaacca attttgcgag gagttcttcg
     1201 cggctcggca agcttgccag agcgtcaaca gctgcaacat caatgacctg accatcagcg
     1261 agacctgctt ttacagtcag cgcttcagtc tttgtttctt tgatgaattc tttgaggatc
     1321 ttagccggag ctacagcgtc ttccgtggaa taagcgagag ccgtcgggcc ttcgaaaatt
     1381 gcatctaatg cgttatagcc gagttcgtca gcggcgatac gggtcaacgt gtttttgatg
     1441 actttgtatt caacaccgcc ttcgcggaac ttacggcgaa gtttcgtgac atcagctacg
     1501 gtaaggccgg agaaaccaac gaaaacagcg ccctgagcgg actgcagttt ttctttcatt
     1561 tcttcaacga cagcagcttt tgccggagaa accggatgat ttgcatatgt ggacatgagt
     1621 tcgatacacc tccttgaact gggatttata tcgtcttagc aatcaaaaac ggcctcatgg
     1681 aaaccacgag gccgaactag tcaaagtcag atatctgcta gtttcatagc ctcagatggc
     1741 ggacatacgt ccattataca taccttcagt ctacagctaa gagagatatt aattttcttc
     1801 tttctttgca gcacctttga tgctgtactg gttgatgtgg atgccagggc ccatcgtgga
     1861 gctaaccgtg atggatttga tgtactggcc ttttgcaccc gacggtttaa ctttgatgag
     1921 tgtatcaacc aaagcagtca ggttgtcaac caatttatct gcgtcgaaag attttttacc
     1981 gatagaagaa tgaatgttgc cggctttatc cgtgcggtat tcaactttac cagctttact
     2041 ttcgctaaca gcttttgtaa cgtccatcgt gacagtacca accttcgggt taggcatgag
     2101 gcctttagga ccgaggattt taccgagacg accaacttta cccatcatgt tcggagtagc
     2161 gattgctacg tcgaagtcga gccagccacc tttgatcttt tctacgagat catcagcacc
     2221 gacatagtca gcgccagcag ctaaagcttc tttttcttta tcgccctgag caaatacgag
     2281 gacagtcttg gatttaccaa tgccgtgggg cagtaccaag gcgccgcgga cctgctggtc
     2341 ggcgtatttc ggatctacgt taaggtttac ggccaattca acggtttcgt cgaatttggc
     2401 agtatccatc tttttcaaga tgtcgattgc ttctgcaaca tcatactgtt tctgtttgtc
     2461 gaacagtttt gcagcttcag catatttctt gcctactttt gccatgtttc ttcctcctta
     2521 tgtggtttaa cgggttaccc cttccacggg cagagcatca tcgaattagt cgacgatttc
     2581 gatacccatg ctgcgagcgg tgccttcaat cattttcata gcagcttcaa cgtcgttagc
     2641 gttgaggtcc ggcatcttag tcgttgcgat ttcgcggact ttgtcgcggc ccaatttagc
     2701 tacttttttc ttgttcggtt cgcccgaagc tttgtccaaa cctgctgctt tcttcaagag
     2761 gacggcagcc ggcggtgttt tcgtaatgaa agtgaaggaa cggtcttcat agactgtgat
     2821 ttcgacaggg ataatgaagc cagcctgttt agctgtgcgt tcgttgaatt ccttgacgaa
     2881 agccatgatg ttgacaccag cctggcctaa tgcagggcca atcggaggcg ccggagttgc
     2941 tttaccagct tcacactgga gttttaccat tcttgctact ttttttgcca tggtgagtta
     3001 cacctcctta cattacaaga tgtggtcaaa cgaatcttgc gattctccca ctcgcggcga
     3061 gttgccgcat ttagaagtat ttattccaga cccttctcga tttgagaata gtcgacttct
     3121 actggagttt ctcggccaaa catattgacg aggcctttga cagtcccttt ttcttcattg
     3181 atttccgtta cgacggccat gcaattttca aaaggacctt cggtgatgcg cacttcttcg
     3241 ccaacttcga cagagatatg cgttttcggt tcgctttcta tgccctgggt cttcagaatc
     3301 ttttcgactt ctttcgtttc cagcgggata ggcttggttg ccgagccgac aaaaccggta
     3361 acacccggtg tattgcggac gacataccag gaacggtcat tgacttccat ttcgatgaga
     3421 acgtagccag ggaatacttt ccgcttgaca gtccgtttct taccgtcttt gatgtcaatt
     3481 tcatcttcca ttggaatgag aatacggtta atgacgtttt ctaaccccat ggatttgact
     3541 ttttgttcca gcgtttgcat tactttattt tcataaccgg aatacgtatg gatgacatac
     3601 catttcatat ctgcttccat ttaagcacaa gcctcctatc cgacaacaag ctgaagtaac
     3661 ttgaacagct gtgaaaatac accatcagct acgccaatga ttaccattac gacgatggta
     3721 gcgacaatga ccataaccgt gtagttgatc aattccttct tcgtcggcca gatgactttt
     3781 ttcatttcta cctttacgcc agataaaaat gatttgcccg ggttcttttt cttgttctta
     3841 ttcttttgtg ctgctttttt agctgaaagt gccatataac ctctcacatc caaataagat
     3901 tagcacaatc ttattcaatc aattatttcg tttctttgtg taatgtttct tttttgcaga
     3961 acgggcagta ttttttcagt tccaaacggt ccggattgtt ctttttgttt ttgttcgtcc
     4021 ggtagttacg ctgtttgcat tctgtgcatg ccaaagtgat agctgtacgc attccttaca
     4081 cctctcttaa aaatagtcac aagcctagtg ttacgtaaga ctgcctttat aatgtatcac
     4141 aacagtcccc tctaagtcaa ggaaattttt ctgtaactta aaaaagccag gcgttggtga
     4201 acgcctaact tcgattattc taacatgtaa cgaccgtcct gtcaagagat ttcccataat
     4261 tccatagcgg gccgcccagc cacagctgtc cgttctggca accgtcctgc gccttccgta
     4321 ggtcggcacc tgacgggcca gaaaggcgcg ggacggcccc tacgcacaag gccgctggcc
     4381 gtgcattttt ttgtgacttg tggcctgcgt ctatcgcggc gcatccgcat ctgaatcgta
     4441 caacgccgcc agggcgtcct ggtatgttcc caagatgcgc cccacctgtg cttctatggc
     4501 gtcgtggctg ccgtggcggt cgcggagcat ggccagccat atgtcttcat cgacgaagag
     4561 gtattgttca taggccgcgg cgatgagttc cttggcggag cccatggcag ccatgccctg
     4621 gccgtcccgc ttcagggctt cgcgaaaggc catcaagccc aaatcaaagg cttcctggaa
     4681 gatgtggacg atgccatggc agacgaaggg gttcgtcaaa tccgcttccc gagcctgctg
     4741 caaaagctgt atatgttctt ggaaattatc ctgccaatcc atagctgcct ccccaaacat
     4801 caaatatcaa acatcaaacc tattataatg gatttccggc aaaaaaagaa gcagccctgc
     4861 tgtcaggagg agacagggct gcttcacgat aaatggcttg ggaatttagc cagagatttt
     4921 ctgttgtgcc agataggttt cttccaggac ctgtttgcct gcaatacccg gctgggtcat
     4981 ttcctgcggc gacaggatga tgtccatctg ttctttcgtc agatagccgt gttcgaggat
     5041 gatttcccgg atcgggcggc ctgtggcata ggcttccttg gccagcatgg acgaccgttc
     5101 atagccgatg tgcggcaaga gggccgtgac gatgccgacg ctgtggtcga cccaggactg
     5161 gcaggctttt ttgttggcct tgacgccttt gagcaggtat ttgacgaagg cgtggatagc
     5221 gttggtcaaa tactgcatgg agtggaagag gttgaaggcc atgaccggtt ccatgacgtt
     5281 caattcgaac tggccgtttt cgacgcccag gctgatggcc atgtcattgc cgatgatctg
     5341 gtagcacgtc tggtcgagga cttcagccat gaccggattg actttgcccg gcatgatgga
     5401 agaaccgggc tgacgggccg gcagcttcaa ttcgttgagg ccgcagcgcg gaccggaagc
     5461 catgaggcgg aagtcgttgg ccatcttgat gagcatgaga gccgtagctt tcatattgcc
     5521 cgatacttcg gcaaagatat ccgtgttatt ggtcatatcg atgaggttgc gggccgtgac
     5581 gaagatatca ccggtcagca tggaaagttt cgtggcgacg gctttgatgt aagccggttc
     5641 ggcgttgagg cccgtgccga cggccgtacc gcccatgttg atgacgtgga cgccttcgag
     5701 ggaccgggaa atgcgtttgc ggccgcgggc gacagccgaa gcataggcgc ccatttcctg
     5761 gccgagcgta atgggaacgg catcctgcaa gtgggtacgg cccatcttga tgacatcttt
     5821 gaattcatcg gctttgacat ccagttccgt ttccagttcc ttcagtgctt ctgtcagctt
     5881 cttgcctttc atgctcaggc agactttgat gctcgtcggg aagacgtcgt tcgtcgactg
     5941 agccatgttg gcgtgattgt tcggcgagat gatgtcgtaa cggccttttt ctttgcctat
     6001 gatttctaag gcccggttgc acaatacttc gttcatattc atgtttacgg aagtaccggc
     6061 accgccctgg atcggatcaa cagggaactg gtccagccac tggccgtcga tgatttcact
     6121 ggcagcctgt accagggcat tgccgatttt cttgtcgagc cgtcccgttt ccatgttggc
     6181 gagagcagct gcttttttga caatggccat ggcctggatg aagtccggat ccagatgttc
     6241 acctgtaata tggaagtttt ccatagcccg catggtctgt acgccataat aaacattgtc
     6301 cggtacattc atttcaccta aaaagtcatg ttctgttctc ataatatcct ccccaatacc
     6361 catcttgcaa tagatgcatt aaatattttg tatacagtat ttaagtaata attatagcaa
     6421 tactgtagta attaaattca tacccctccg gcgtccccac gccgttacat acacaaaagg
     6481 cgctgttgaa attgctttca acagcgcctt tgccgtacgt actatgaatt tacctatatc
     6541 atagtgcata gatgggcaga tgtcaatatt tattccgcca ctttatgttt tatttagact
     6601 tcttgcagcc ttaggcttct tcgatggccc cagccggaca ggagttcatg gcttcctgag
     6661 cagcgtcgtt gttagcttcc gtcgattcag cataggcttc agccataccc gtatcatcgt
     6721 tcatacggaa cacttccggg caagtcccag cacacatacc acaggcaata cacgtatcgt
     6781 ttactttata tttcataatg agtcactcct ttgtccggac cgtctatccg gaaaacttag
     6841 tccagtaagt cattccttac tgtgcctata gtataccctg ttttcggcca aagggtcgtt
     6901 gtatttacaa cgctttagca aattagcgat acgatgcctg ataaatagca acgcagtctt
     6961 cttcactcat gggcatgcgg tcggccatga ataatcccgc catggccgtc ttggcattat
     7021 gggccatttt atggaattca tcgggactga tgccgtagtc ggacatcttg aggttggcga
     7081 cgccgcattt ttcctgcaat tctttcaagg cgtcgaggaa gtcttccggt ttggccgcat
     7141 ccggacgccc catatggcgg gccaggatga tgaaacggtc gtcacagacg tggatgtcga
     7201 tcatgtgcga gaaataagcc aggctgatca taatcaggcc ggcgccgtgg ggcaaggcct
     7261 ggtgataggc gctgagggca tgttccagcg agtgttcact catgttggag ctgaggcaca
     7321 tttccaagcc gccgagatag ttgccgaaag ccacgcgttc ccgggccttc atgtcgatgc
     7381 cattctggac ggctgtcggc aagtagggtc taatctggta gatgggttcg aagtcgcaca
     7441 tttcgctcat gagattggcc taattgtcca gatagccttc gatattgtgg aac
//
LOCUS       DAWCYS010000059         7429 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_629_length_7429_cov_6.140765, whole genome shotgun sequence.
ACCESSION   DAWCYS010000059 DAWCYS010000000
VERSION     DAWCYS010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_7429_cov_6.140765"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1666 a         2218 c         2082 g         1463 t
ORIGIN      
        1 tgtaaggggc ttttggacag ccctttgaga ataaggctat cctgatttca ggagggaact
       61 taaacagcag tcttctcaag aatctcccgc ttctataagc gggagaggtt caatacgatt
      121 ctcgtgctgg cagggtattt gaaaatcgta tcccaccggc gagccgcctg agccctggac
      181 ggacgacgtc gtatctgatg acgaccatgg aaaacccgcc gtcagattga tgaaaagggc
      241 tatgaaaccg atttcaagga ccggggcatt acgaaagtcc tgaaatacgg tatcgccttt
      301 tacgggaaac aggtcgtctt agtgtaaaaa ttgagtcaaa agaggtcgaa gcccaaggga
      361 aaatctttgt gcaacggcct ctttcatgat ataatgaacg ttagtctatt tatttctatt
      421 ttatggtatg tatggaagac aagggaggca gtgaagatgc gggcaatggg aatcgatccg
      481 gggacggcta tctgcggttt tggtgtcatc gacgccgacg gcagccgtct gaaaccggtg
      541 acttatggga ccatccagac gacgccggac cataccgatg ccgaacggct ggtcatgctc
      601 tttgacggcc tcaatgaact ctatcggaaa tataagcccg acgtcatcgg tgtcgagcag
      661 ctctttttta accgcaacgt cacgacggcc attaccgtcg gccaggcgcg gggcatcatc
      721 ctcctgacgg cccagcaggc gggcatccct atcctggaat tttcaccgct ccaggtcaag
      781 cagggtgtca ccggctacgg ccgggcgacg aaggaacagg tcatcgacat gaccatgcgc
      841 cttctcggca tccgccagaa aatcaagccc gacgatgcgg ccgacggcct ggccatggcc
      901 atttacgcga tttattccag tcattctatg agtctgaaaa gggaggtgca gttatgatcg
      961 gttatctgaa aggcgtgatc agccacatct tcgtcaattc ctgtttcatc gacgtccacg
     1021 gcgtcggcta ccggacctat gcaccggctt cgacgctgga cactctctgt gtcggccagg
     1081 aagccatgct ctatacttat atgagcgtcc gcgaagacgc catcgtcttg tatggctttg
     1141 ccacgcagga tgaatacgat ttgttcatgc tcctcatcgg cgtcaacggc gtcggcccca
     1201 aagtggccct gggcatcctc agcgccggca cggccgacag tttccgcctg gctgtccatc
     1261 agaaggacat caagggcctg accaagatgc ccggtatcgg caagaagacg gctgaacgca
     1321 tcgtcctgga attgcaggat aagatcggtc ccgtcgccag tgaagacgcc ggggctgcgg
     1381 atctgggcgg ttcggccgcg ccgggcctgt cgggcatcct ggcagaaacg ctggcagccc
     1441 tgaccagtct cggctacagc gaacaggaag tcctgcccgt cgccgaatcg cacctggccg
     1501 gctgtacgac agtggaacaa ctgctgaaag agacgctgaa ggccttgggg agtgggaggt
     1561 aagccatgga agaaaatcgc atcatcgaga cgacagacct gccgggcgat tcctggcagt
     1621 atagcctgcg tcctcattat ttacgcgaat atattggtca ggataaggtc aaagacaatc
     1681 tggaagtttt catcaaggct gccaaactgc gcaaggaagc gctggaccac gtcctcctct
     1741 acggcccgcc gggcctgggc aagacgacca tggccggtat cattgccaat gaactgggcg
     1801 tcaacctgcg catcacctca gggccggcta tcgaacggcc tggcgaccta gcagcgctcc
     1861 tgaccaatct ccaggaacgg gatctcctct tcatcgatga aatccaccgc ctgtcgcaca
     1921 gtgtcgaaga agtcctctat gcggccatgg aagactatgc tctggatatc gtcatcggca
     1981 agggccccag tgcccggtcg gtgcgcatcg acctgccgcc gttcaccctg gtcggggcga
     2041 cgacccaggt cggccgcctg gcaccgcctc tgcgcgaccg cttcggcgtc atctgccgcc
     2101 tggaattcta taaaccggag gacctggtcc tcatcatcaa gcggacggca gatatcctcg
     2161 acatggccat cgatgacgac ggcgccttgg aaatcgcccg ccgctcgcgg gggacaccgc
     2221 gtattgccaa ccgcctcctg aagcgcgtcc gcgacttcgc ccaggtcgcc gaagccgcga
     2281 cgattacggc gtccctggcc aacgatgccc tgaaccggct ggaagtcgat tcgtgcggcc
     2341 tcgaccggac ggaccgccgg gtcctgcagg tcatcatcga taaattcggc ggcggtcccg
     2401 tcggcctcga caccattgcc gccgccatca gtgaatccgt cgatgccgtc gaagacgtgt
     2461 acgaaccgtt cctgatgcag cagggctttt tgaaccgcac gccccggggc cgcgtcgtca
     2521 ccgacgccgc ctaccgccat ctgggcctgc ctgcgccatc agaaggatag gagggacgat
     2581 tatggcgaaa ttactcatcc ttacgggtct ctttttcatc gtcgccggcc ttttatggat
     2641 ggccggtgat tccctgggac tggggaattt cctgggccat ctgcctggcg atatttcctt
     2701 taccaaaggt aacacgtcct tccattttcc catcgtgact tgcctcatca tcagtgcagt
     2761 ccttacgatt gtcctcaatc tcttctttcg cctgtgagga ggatttttat gtataaaaag
     2821 aagatagccc gcctggcctt atgcacggcc ctctgtgccc tggtgacgac gagtgtcttt
     2881 gcgtcgccga ccaaggccaa ggccaaaagc catccccgcc agcccgtcaa agcggtgcgg
     2941 cagacggcca aggcgccggc tggatataca cataaacaag ccgtccacga tagcgctacc
     3001 ctgcgcatcg gcatccgcga aggccggggc agtgtggccg tcaccgggcc gcagggtctg
     3061 ggcgtctatc gcggcgacat gctctggaag aaagcggcgg ccaacgtgcc cgtcaccatt
     3121 gccctgtcgg ggacgaacct gaccgtcaac ggcgacatca gtaccgtacc ggcccaggtc
     3181 cggtccctgg tccacggcgg ctccgtcaag atcactgacg gctacgccta ccgcggcgcc
     3241 ctggaaatga tgaaatctcc cggccgctgg ggcctgaccg tcgtcaacgt cctgccggtg
     3301 gaacagtatc tctacggcgt cgtcggcaag gaaatgtcgc cgtcgtggag cgaagaggcc
     3361 ctgaaggccc aggccgtcgc cgcccggacc tatgccattg cccataagag ccgcttcagc
     3421 cagcgcggct tcgaccttac cgacgatacg tcgagccagg tctatgccgg catcaatggc
     3481 gaatcgccgt cgattatcaa ggccgtcaat gagacgaaag gggaaatcct gacctatcag
     3541 ggcaagccca tcgatgcctt tttctgttcc accgccggcg gctggactga agacagtgaa
     3601 aatgtctggg ggtcctacgt accctatctg cgcggcgtcc gcgatgccag caacgccatg
     3661 ccggggtacc gctggtcggt gacgacgacg ccggaacaga tggcggccaa actgagtgct
     3721 gccggcaaag gcgtcggccg tatcaaggcc atcgaactgt cgccgctcat gaagcggccc
     3781 atgtccatga cggaccgcgg cgtatccggc cgtgtccagg aaatgatcat caagggcacg
     3841 aacggcaatg tcaccgtcaa gggctcggcc ttccagtcca tctttggcct gcgcagtacg
     3901 ctttttgatt tctacagcgg ccgcggtacg gcaccggatc ccgattccgg cgccgcggtg
     3961 cgcaagagcg acttcatcgt caaagccggc cagcctgtga ctatctacgg cttcggctgg
     4021 ggccacggcc tgggcatgag ccagtacgga gcctaccaga tggccaaaga acatgtcaat
     4081 gaaaagaatt attaccgcaa tatcctgacc cattactata cggggacgaa gattgaaaaa
     4141 ttatattaag ctaaaaagag gaagattatt ttgaaactga cagattttta ctacgattta
     4201 ccgaaagaac tcatcgccca gcatccggct gaaccgcggg accacgcccg cctcatgctc
     4261 tatgaccgtc agaccggcgc cgtcgaacac aagtatttct acgacctcgt cgacgaactt
     4321 cacccaggcg acgtcctggt cttcaacgat tcccgcgtca tcccggcccg gctccacggc
     4381 aaacggatgc cgactggcgg aaaggtcgaa gtcctgctct tgacgcctgt cggcgacgac
     4441 cgctgggaag tcctggtcaa gccgggcaag aaggccctgc cggggacgac aatcgagttt
     4501 cctgaaggcc tgacgggtac cgtcgaagac cggaccgatt tcggcggccg tatcatcaag
     4561 ttcgcctacc agggcaattt cgacgacatc atcgacaaga tcggtgaaat gccgcttccg
     4621 ccgtatatcc acgaaaagat ggaagacccc aatgaatacc agacggtcta tgccaacgaa
     4681 cgcggcagcg cagcggctcc gacggcaggc ctgcacttta cggaagaact gctccagaag
     4741 atccgcaata aaggcgtgga agaagtcttc gtcactctcc acgtcggcct ggggacgttc
     4801 cggccggtcg aagaagaaaa catcgaagac caccagatgc acagcgaatt ctacagcctc
     4861 acgcccgaag cggctgccaa gatcaacaag gccaaggccg aaggccgccg ggtcatcgcc
     4921 gtcggcacga cgagcatccg cactctggaa agcgccggta cgacagggac cctgcaggcc
     4981 ggttcgggct ggacgaatat cttcatctat ccgggcttca ccttccatat cgtcgacgcc
     5041 ctggtcacta atttccatct ccccgaatcg acgctgctca tgctcatcag cgccttgtcg
     5101 acgcgggaaa aaatcttgaa agcctatgaa atcgccgtcc aggaaaaata ccgtttcttc
     5161 agtttcggcg acgccatgtt cattcactaa gggaggaaaa gctatatggt cattacctac
     5221 gaacagcagg ccgaatgtga cggcgcccgc gccgggatac tgcgcacgcc gcacggcacc
     5281 ttcaagacgc ccatgttcat gcctgtcggc acgcaggcga cggtcaagac gctgacgccg
     5341 gaagaactct acgatatggg cgcccaggtc attctcagca atacgtacca cttgttcctg
     5401 cggcccggcc acgacctggt ccaggaagcg ggggggctgc ataaattcat gaactggaaa
     5461 caggccatcc tgactgacag cggcggcttc caggtcttca gcctgggcca gatgcgcaag
     5521 atcaccgaag aaggtgtcac cttccggtcc cacctggacg ggtcgaagaa attcctgtcg
     5581 ccggaagttt ccatggatat ccagatggcc gtcgattcgg acatcgccat ggccttcgac
     5641 gaatgtatcc cttatccgtc ggactatgaa tacacccaga tgtcgacgga acggaccagc
     5701 cgctgggctg cgcgctgcct ggaccaccat cacagcgata cgcagggcct gttcggcatc
     5761 gtccagggcg gcatgtacaa ggacctgcgc aggcagagct gccaggacct ggtcgacatg
     5821 gatttcgacg gctatggcat cggtggcttg agtgttggcg aatctaagga tataatgtat
     5881 gatatattag agcatacgac accgtatctg ccgaaggata aagcccgcta cctcatgggc
     5941 gtcggcacgc cggactgcct gctcgaaggc gtcgcccgcg gcgtcgatat gtttgactgc
     6001 gtcttcccga cgcgcgttgc ccgcaacggc atggccatga cccatacggg ccgcctgacc
     6061 gtgcgcaacg ccaagtatgc ccacgacttc catcccattg aagaaggctg tcagtgttat
     6121 acgtgccgca attattcgcg cgcctatatc cgccatctct tcaaggccga ggaactcctg
     6181 gcataccgcc tggtcagcat ccacaacctg tacttcctgc tgcagttcat gcgggatatg
     6241 cgccagtcca tcttcgacgg cacattccgc cagttccgcc aggatttctg gaaacgttat
     6301 caagacgcgt aagcgcttgc atatagacta tttttttatt tcacggaggt gtaagacatg
     6361 gatattatgg aacagctcaa tgctttttgg cctattatcc tcatggtcgt cattttctac
     6421 tttctcttat ggcgtccgca gaagaaacag cagaaaaaac ggcaggaaat gctggaaagc
     6481 ttgaagcccg gtgctaagat cgtcaccgtc ggcggtgtca tggggaatat cgtttccctg
     6541 cacgacgatt acctcgtcgt ccgcattgct gaccgcgtcg aaatcaaagt cacccgcgct
     6601 gctgtagccc aggtcctggg caaggccgaa ggcaagaaag cagccaaaaa agcggaaaag
     6661 gctgaaaaag ctgccgaaaa agccgctgaa accaaagcag aagctccggc tgaagccaag
     6721 agcgaagccg ttggtgaaac gaaggaagcc gaagccaaag atggccagta aaaaagaact
     6781 gcgcaaagcc atgaatgccc gcctccacgg gctgacggcg gaagaaaagg cagcggccag
     6841 tgccgctgtc tgcgcccgtc tggccgccct gccgcagtat cggcaggcca gcagtatcat
     6901 ggcttttctc catatgcccg acgaagtcag cctcgatgcc ctgatcaagg acgccctggc
     6961 tgccggcaag gaagtctacg tccctgtctg cgtcgataaa cagcatatcg aagggcggcg
     7021 cctgtatacg ctggaacagg ctgtctgcgg cgcctacggc atccggacgg caccgccgga
     7081 caatccgcgc atcgaagccg atgagctcga tgtcattttc gtgcccggcc tggcttttga
     7141 cgccaagggc caccgactgg gccatggggc gtcctattat gaccgcttcc tggccggaaa
     7201 gggcggagca gccgcgattg ctgcggcctg ggatgtccag atcatccccg acgtgcccgt
     7261 cgaagcccat gacgtcatca tgaccgatat cgtcacggac aaacagcatc tcattatcca
     7321 tccataaaaa actattgaag attacccaga ggggggagta ttgttgcgga ctcgtaacat
     7381 gataaaattt ttcggggcag tcatcatcat cctggccgcc tttgttacc
//
LOCUS       DAWCYS010000060         7304 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_636_length_7304_cov_6.374396, whole genome shotgun sequence.
ACCESSION   DAWCYS010000060 DAWCYS010000000
VERSION     DAWCYS010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_7304_cov_6.374396"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1750 a         1939 c         2048 g         1567 t
ORIGIN      
        1 aagagaagaa agagaaattt agcctagccg atgagggcac cgctgccgtt ggacgaaacg
       61 gagtcgtcga cgaggcccat gatggcgagc ggagccaggc ggatgatcca ctggacgcag
      121 gtcgtgacag cgtcggcaca gtcatcgagg acggctttcg acgattcgga gacgcggcgg
      181 acggcaacgc cgaacaggca ggcccagccg aggataccga tgtagttgcc cgtcatcagg
      241 gcgtggaccg ggttgtcgac gatgttcttg aggatcgtcg tcaagacttc ggcaatgcct
      301 tccggtgcct tattgctggc agccgatacg tcgagctgca gcgtcagggg gaagacgaaa
      361 ctgatcatga cggcaaagaa agccgctaaa aaagtaccga tgagatacag gatgatgaca
      421 tctttgatgt tcgattcgtg gtcttcctgt ttgcggctca gtgcagcggc gaccaggacg
      481 aagaccagta ccggggcgat gcccttcaag gccccgacga agatgacgcc gagcaaggcc
      541 agttccttcc ctgctgccgg gaaagccata gccacggcga taccgatgac caagccgatg
      601 atgatctgct tgatcaggct gatgcggttc aatgcattcc ataaactatg cataaataaa
      661 acctcctacc ctaattccat gttacaaatg tataatgtat gaatacactt gtttattata
      721 aagcaaaagc cgtaaatttt caataaaaaa aagaaatctc atataaagct ttttgttatc
      781 acgtaggttt tctttgcctt tgatgtatga tgtttgacga aaggctctcc tcataggaga
      841 gctggccgcc aaaggcggtc tgagaggttg atttctcagc atttgtagtt tttagtggat
      901 agcgctaaat ttaaaaccat ccgctaactg ctagccgctg actgctaaaa aaaggggctg
      961 tcgcacgtgc gacagtccct tttttcatat atcaaacttt tattcactta atacggcagc
     1021 gcagcgggcg cagagggtcg gatggttcgg atccgagccg acagtgtcgc tgtgaatcca
     1081 gcagcgttcg catttttcag ctgtcgacgg agcgacgacg actttcatgt cgccgtggtc
     1141 ttcaccggca acggcttcag ccggggcttc gcctttgacc agtttggctt cgctgacgat
     1201 gagcagcgtg gccagctgat cctgccactg gctgaggaaa tcgtagtctt cgccatcggc
     1261 atagatggtg acagctgcgt ccagggcatg gccgatcttg tgttccttac gggcgccttc
     1321 gaggacacgc gtcaaatcgc ggcggtacga caggaactga gcccagcggg catcgaggtc
     1381 agcgtcgagg tattctttgt gagccttggg ccaatcggtg agcaggacac tttcttcctt
     1441 gccttcgaca gcgggcatgt tctgccatac ttcttcggcc gtgaaggaca ggaccggtgc
     1501 gacgatcttg accagcgtat cgaggatgat gtacatagcc gactgggcgc tgcggcggat
     1561 atgggaatca gccttttccg tgtacaggcg gtctttgatg acatccaggt acatggcgct
     1621 caaatcgacg gtgcagaagt tgtggatagc gtggtacatg acgtggaact ggtagttttc
     1681 ataagcgtcg gtgacttctt cgaagacctg ttccaggcgc aggagagccc atttatcgag
     1741 gtccgtcatg tcctggtagg cgatcatatc cttcgccgga tcgaagtcgg ccaggttgcc
     1801 caggaggtaa cggaaggtgt tgcggatctt gcggtagacg tcggagagct gtttcaagat
     1861 cttcggcgac aagcggatat caccctggta gtcagccgac gatacccaca ggcgcaggac
     1921 gtcagcgccg tatttttcga tgacttcctg gggagcgacg gtgttgccga cggatttact
     1981 catcttgcgg ccttcgccgt cgaccgtgaa gccgtgggtc aggaccgatt tgtacggagc
     2041 atagccgttg acggcaaccg acgtgagcag agagctgttg aaccagccgc gatgctggtc
     2101 cgagccttcc aggtacattt cgcagggata atcgaggtcc gggtcatttt tcagtacgcc
     2161 ctgatgggtg cagccgctgt cgaaccagac gtccatgata tccgtttcct tgcggaagtg
     2221 cgtgccgccg cagtgcgggc atttatagcc ttccggcatg agttccttga cgtcgtgcat
     2281 ccaccacgta tcggagcctt ctttggcgaa catcttctgc aaatgagcga tggtttcatc
     2341 gttgatgatg tgttcgccgc agtcttcgca gtagaagata ggaatcggca cgccccagac
     2401 acgctgacgg gaaatgcacc agtcaccgcg gtcgtggatc atattgtaaa tgcggtcatg
     2461 gccccaggac ggaatccatt tgaccgtgtc gatggcatcg agggctttct ggcggaagcc
     2521 gtcgaccgaa gcgaaccact gttccgtagc acggtagatg atcggttcct tgcagcgcca
     2581 gcagtgcgga tactgatggc ggaacgtgct cttggcaaag agggcgccca gttcagccag
     2641 tttcttgatg accggtacgt tcgtatcgaa gacgaactgg ccttcaaagc cgggaacctt
     2701 gtcggtgtaa cggcccgtgc cgtcgactgt gcccagcggc ttgaggcccc aggcagcata
     2761 ttttttgcaa acatcaaagt cgtcctggcc atggtccgga gcggtatgga cgaggccggt
     2821 accagcttcg agagtgacgt ggtcgccgag gatgatgggg actttacggt catagaaggg
     2881 atgcttgaag accaggcctt cgagctgttt gcccgtcatg acgtcgggca ggacttcgta
     2941 gtcgtcgact ttgccggctt tcatggtcgg ttcgaccagg tctttggcca tgagcaggta
     3001 ttcgtcgccg atgcgtaccc agacgtattc aaagttttcg ttggcactga tggcgacgtt
     3061 gcagggaatg gtccacggcg tcgtcgtcca gatgagggcg aagactttgt ccggatcggc
     3121 acctttcggc atatggcagc tgaggtcgac ggacttgaat ttgacgtaaa tggagaagga
     3181 tttatcatcc ttgtattcga tttcagcttc agccagggcc gtttcgcagt gcgggcacca
     3241 gtaaacggtc ttcaggcctt tgtagatgta gcctttcttg gccatttccc cgaagacgcc
     3301 gatctgagcg acttcgacgg gcttgcgcag cgtcaggtac ggatggtccc agtcaccgag
     3361 gacgccgagg cggatgaagt ctttcttctg ttcttccacg cgggccaggg cgtagtcacg
     3421 gcatttggca cgcaagtcga ggggggccat ttcgtggcgg ttcaggcccg tatctttgat
     3481 gacggcgtgt tcgataggca ggccgtgcgt atcccaaccg ggaacgtatt tcgtatagca
     3541 gccctgctgg gtcttatatt tgaggataat gtcttttaat actttattca atgcatggcc
     3601 gatgtggatc ttgccattgg catacggagg accgtcgtga agaatgaact tgggcgcacc
     3661 gtcccgtttc ttcaagcgtt tttcataaat cttgttgtcc tgccagaatt tcaaaaagtc
     3721 cggttcccgt ttcggcaagt tgccgcgcat ggggaaatcg gtctgaggca gatgtaaagt
     3781 cttaccgtaa tccattgtgt ttcctcctct gaatctgtga aaaaaaaata acgtctccgt
     3841 cccaaaaagg gacgaagacg taatcaccgt ggtaccaccc tattgactgc cggagcagcc
     3901 actttgacgc gcaataacga cgcgacccgg cagctggttc gcagctgcag ctcccaagtg
     3961 atccgccagc atacggcctt tgccgggctc tcactatccc cggttcgctg aaaaggcaca
     4021 ggcgacactg gccgtcttgt tcatcgcttt tcattttttg tgacaattac ttgtacatag
     4081 acgtattgta aaggcttatc ccccatttgt caagaggctt acttccaata ctggaccgta
     4141 ctttcatcaa tgccgatgga agccgccttg aagacagggt tcctgccttc cttcttctgc
     4201 tgctggtagt cgcgcatggc cttgacggcc gtgttgccca ggatgaagca ggccgggatg
     4261 ttgatgaagc acatgacggc catgaggata tcggcaatat cccagacagc ggccatggac
     4321 atgccggcgc cgacgaagac gacgaggacg cagacaatgc ggaaagtatt gacgaagccg
     4381 tgggacggcg cgcccttgtg gatgaagcgg atgcagttat cgacatagaa caagttgccg
     4441 atgagcgtgg aaaaaccgaa gagcagcatg cagatcgtaa tcaggagcgg accgaagccg
     4501 ccgaagacgg ccgtagctgc attctggacg taagccgcac cggctacttc cttagtgatg
     4561 ggcacgcccg agaagaggcc catgaaagcc gtagcactgc agaccaggag ggtatcgatg
     4621 aagaccgaca gcatctggac caggccctgt ttgaccggat ggctgacatc ggctgcggct
     4681 gctgcattcg gtgccgaacc gataccggct tcgttggaat agaggccgcg cttgataccg
     4741 tagaccatgc aggagccgga cacgccgccg aggatggact ggaagtcgaa ggcatcggcg
     4801 aagatcatgg cgaagacctg cggcaggctg gtaatgtgga agacgatgac gatgagcgct
     4861 gccaggatat agatgccgcc catgagcgga accaggaccg atgtgacctt gatgatgcgc
     4921 tggccgccac cgatgaggca gtaggcgacg agagccgcaa aaatggcgcc gatgatccac
     4981 ggcgtcgtcg tcgggtcgta gaagctgtag accaggaacg tcgactgcat gttgaacgag
     5041 cagagcaggt tgaagccgcc ggcataagtc acgatcaggg aaaaggcgaa gaggacgccg
     5101 agccagcgct tgtgcatggc cgtttcaatg tagtaggccg gaccgccgta gctgtggccc
     5161 aggggattgc ggcgcttgta aatctgggcc agcgtgcttt caatgaaggc cgtagccccg
     5221 ccgatgatgg ccagaagcca catccagaaa acggcaccgg gaccgcccag gcagattgcc
     5281 gtcgagacgc cgatgatatt gcccgtaccg acacgggacg ccgtcgaaac catgagggcc
     5341 tggaaagacg atactttccc ggcttccgtc ggtttttcca tgacgacgcg gatactttcc
     5401 gggaacaggc gcagctggac acagcgcgtc ttccaggaaa aaataaggcc ggctgcaatg
     5461 aggatgacca ttaagatagg ataataaagg acatcatcga ggtagttcag gacagccata
     5521 atcatagaat cacatcctta ccataaaaat aaaaatataa ataaaaatta aaaaaagtcc
     5581 tataaaaagg atacggtatg atgtgataat ctgtcaatag gctatggatg gtttgtatca
     5641 ggccgttcct atcttcccgg ccctgtagta taatgaaaca gaaaggcagg gatttacatg
     5701 acagctattg aacaagtaat acagaaggct atcgccttcg accacggcga tgcccggcgc
     5761 atccatcatt tcctcaaggt ctacgcctat gccgacacca tcggccgcct ggaaggactg
     5821 cccgaagacc agcaggacat cctgcgcacg gctgccgtcc tccacgacat cggcatccac
     5881 gcagccgaag aaaaatacgg ttcctcgtcg ggcaaatatc aggaaatcga aggacccgcc
     5941 ccggccaggg ctatcctgga agaactcggt gctgacgaaa agctcatcga ccgggtctgc
     6001 ttcctcatcg cccaccatca cacctacgac ggtgtcgacg gactggacta ccagatactc
     6061 ctggaagccg atttcctggt caacgcctac gaagaccagc tgaaaccgga agccattgcc
     6121 accttccgcg acaaggtatt ccgcaccccg tcgggcctgc agttattgaa agacacctac
     6181 aatgtatgaa aggggtaatt acgatgacaa atattgaaaa attcgtcgac atggtaaaaa
     6241 attccgataa catagtcttc ttcggcggcg ccggcgtatc gacggaaagc ggcatccccg
     6301 atttccgcag caccgacggc ctgtataacc agcactataa atacccgccc gaaacgatcc
     6361 tcagccactc cttcttcatg gacaacacgg aagaattcta ccgcttctac cgcgacaaga
     6421 tgctggccct cgacgcccag cccaacatgg cccacatcaa gctggccgaa ctggaaaaag
     6481 ccggcaaatt gaaagccatc gtcacccaga acatcgacgg cctgcaccag aaagcgggca
     6541 gtaagaatgt cctggaactg cacggcagcg tccaccgcaa cttctgcatg cactgcaaca
     6601 aattcttcga cgccgaatac atgaagaatt ccacaggcat ccccaagtgc gacgcctgcg
     6661 gcggcaccat caagcccgac gtcgtcctct atgaagaagg cctggacaac gacgtcatcg
     6721 aacagtccct gtactacatc agcaacgcag acatgcacat cctcggcggc acgagcctcg
     6781 tcgtctaccc cgctgccggc ctggtccgct actaccgcgg ccataaactg gtcctcatca
     6841 acaagtcgag caccgacatg gacagcgccg ccgacctggt cctccacgac ccgattggcg
     6901 aagtcttcag tcacgtaaaa ttataaagaa aaggtaaact tccttctcat ttgacacaca
     6961 gtcataacac atcgtagtat actagaggca atgtataatt ttatgcactt ttctgcatac
     7021 gcaaaaagaa tgagaagaaa gaaggtcttt tattcgtgaa gaaacaagta gtctgtaccc
     7081 tggcagccgc ttctgacgaa ggcaataatc cctatgtcga tttggccaaa gtggactaca
     7141 acttcggcag caagaactgg gaactcagcg tcggccgcaa tgacgtctac cgcatcggcc
     7201 gcgacgccta cggcctgcag tacttcgatg ccctcgacgg cgccgaactg cgctatcaca
     7261 acgataactt ggccatcacc acgtacggca aattcaagga cggc
//
LOCUS       DAWCYS010000061         7302 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_637_length_7302_cov_6.170277, whole genome shotgun sequence.
ACCESSION   DAWCYS010000061 DAWCYS010000000
VERSION     DAWCYS010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_637_length_7302_cov_6.170277"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2016 a         1848 c         1680 g         1758 t
ORIGIN      
        1 aagccctggg ccgggccggg gtgggccagc tcatcctggg cgatgccgac gtcttcgacc
       61 cgtccaatgt caaccgccag ctcggggcga cggtccagac cattggccgg ccgaaagtcg
      121 aagtcatgaa ggaacgcctc ttgtccatca atcccgacat cgacgtcgag acccatgcga
      181 cgttctatct gcctgaaagc catcagggct tcatcgccca ttccggggcc gattacgtcg
      241 tcgatgccgt cgatacgatg agcgccaaaa tcgccatcat cgacgaagcc tatcacagcg
      301 gcatccccgt cgtgtcctcc atgggggccg ggaataagct ccatccggaa tatttccagc
      361 tcgattacat cgaaaagacc agcgtcgatc ccctggccaa gatcatgcgc cgggaactga
      421 agaagcgccg tatccgccgc atcaaggtgg cctattcgac ggaagtcccg cataaaccct
      481 tctcggacag cgatgaagtg cgccccagtc cgggcagcat ttcctttgtc ccgtcgacgg
      541 cgggcatgat cctggccggt gccgtcgtgc gcgacctcct gaatctggat taaaagaaaa
      601 tttttgctag ttatacacaa gggctgcgtc tataaaagcg cagctcttgt gttgtccttt
      661 caaggcctgc cagccatttt gataaaaact aaccttaact tgtccacaac atgatggagt
      721 atgtacacgt tgacattcgc tactatatat agtatcatag ggcatgtgaa aaacggaccc
      781 tgtaccatat atcttgtagc aggtatggga aaatgtacaa tttgtatact atatactgtg
      841 gaggtagaca tatgttagaa gtaatcaagc gcaatggaga acgagtgccc ttcaatcgag
      901 ataagatcac cgtagcgatt gaaaaggcca tgaacagcag cagcggtatt tacgaagaag
      961 ggcaggctgc caagattgcc ggtgaaatcg aagcgtatgc tgcttccatc cagaaggacc
     1021 tgaccattta cgccatcgaa gaccaggtct actaccgttt gttgaaatac aacaatccgg
     1081 ccacggccag ggcttacgaa aactacaaga ccatccaggc cttcaagcgc cagaccaata
     1141 cgaccgacga cgatgtcttt ggcctgctga acaacaccaa cctggaagtc ctcgacgaaa
     1201 actccaataa gaacggccac gtcgtatcga cgcagcgcga cctcatcgcc ggcgaagtgt
     1261 ccaaggacat tgcccgccgc aaactgatcc cggccgacat cgtccaggcc cacgacagcg
     1321 gcgccatcca tttccacgac atggactaca tcattcagcc catgttcaac tgctgtctca
     1381 tcaacctgga agacatgctg gccaatggta cggtcatcaa cggcaagaaa atcgatacgc
     1441 cgaaatcctt ccaggtcgcc tgcacggtga cgacgcagat catcgcccag gtcgccagcg
     1501 gccagtacgg cggccagtcc atcaacggca tcgaccgcat cctggcaccg tacgtccgca
     1561 agtccttcaa gaagtacatg aaagccgtcg tcggagaaca gcgcgaaatc tacggcatcg
     1621 aaccggatat ggaaaaggcc gaagaaatcg cctggaagcg gacgaagaag gaaatcaagg
     1681 acggcatcca gaccatccag taccagatca acaccctcat gacgaccaac ggccaggcac
     1741 cgttcgtcac gctcttcatg tatttccagc cggattacga atacgccagg gaagcggccc
     1801 tcatcgacga agaattgctg cgccagcgca tccagggcat caagaacgaa gccaacgtct
     1861 acgtcacgcc ggccttcccg aaactcattt acgtcctgga cgaacacaac gtccgcgaag
     1921 gcagcccgta cttctatttg accaagctgg ccgctgaatg tacggcgaaa cgcatgtatc
     1981 ccgactatat ttcggccaag aagatgcgcg aatcctatga aggcaacgtc ttcagcccca
     2041 tgggctgccg ctccttcctc agcccctgga aagatgaaaa gggcaactac cagttcgacg
     2101 gccgcttcaa catgggcgtc gtctccctca acctgccgca gatcggcatc ctcgccgacg
     2161 gcgacgaaga aaaattcttc cagatcctcg acaagcgcct ggaactctgc aaaaaagccc
     2221 tgctcctgcg cgtcgacctc ctcaagcgca tcacgtccga cgtatcgcct atccactggc
     2281 agtacggcgc cattgcccgc ctgaagcccg gcgaaaccat taaaaaattc atctacggcg
     2341 gctatgcgac cttgtccctg ggctacatcg gcatctacga agcgacggta ctgaccaagc
     2401 actgctcgca cacggcgccg gaaggccgga aattcgccat gcgcatcatg gacgacctca
     2461 acgcccacat ccagaagtgg aagaaggaaa cgaacatcgg cttcgccctc tacggcacgc
     2521 cggcagaaaa cctgaccaac ctcttctgca aaatcgacaa ggcccgcttc ggctccatcc
     2581 ccgacgtcac cgacaaaggc tactatacca acagctatca cgtcgacgtc cgcgaaccga
     2641 tcaacgtctt cgacaagttc tcctttgaag ccgactttga agacaaatcg acaggcggct
     2701 gcatcagcta tgccgaaatc cccaacatga cccataatat cccggccgtc ctgaccatgg
     2761 tccagtacat ctacgaccac atcacgtacg gcgaattcaa taccaaactg gactactgcc
     2821 atgaatgcgg ctttgacggc gaaatcatcc tcaacgacga caacgaatgg gaatgcccgc
     2881 gctgccacaa caaagaccgc aacaaattga ccgtcatccg ccggacctgc ggctatttgg
     2941 gtgaaaattt ctggaacgaa gggcggacgc aggaaatcaa gaaccgcgtc ctccatatct
     3001 aagaggtgac ttgacttgca ttatgcacag atgcggcaat atgacattgc caatggaccg
     3061 ggcatccgct cgacgatttt cgtcaccggc tgctcacgcc actgcttcaa ctgtttcaac
     3121 ccggaatacc aggaccccag ggccggacag gaatggacgg acaaggaaat ggaacagctc
     3181 ctggcctggc gcagcgcccc gaccaacagc ggccagaccc tcctgggcgg ggaacccatg
     3241 gaaaacaccg acggcctgac gcagatcgtc caaaacgtca aacaagccat gcccgataaa
     3301 tcggtctggg tctactcggg cttcacctat gacgaaatca tccaggaccc gcagaaaaaa
     3361 gccctcttgg acgtctgtga cgtcctcgtc gacggcccct tcgtcgacga cctgaaagac
     3421 ccgggcctct acttccgcgg ttcctcgaac cagcgcgtca tcgacctcaa agcgacaaga
     3481 accagcggcc agatcaccct ggtatggccc gacggccgct gatgacaaat aattaataaa
     3541 aattatccgt tgatattatt tacaatggaa gcctttcctg ctataataac tgtaggggcg
     3601 accacgtggg gcgcccgcca cagacgtgtg gtgctatccg ttaccgtata aatggaatgc
     3661 tttgcggtct gccaggctgg cgattttgtt cgacggcctt gaatttcaaa aaaggagggc
     3721 ttctcatggt tgttgaatcg gtgaaaaaag ttaagaatat cgtctctgaa gtctttaaag
     3781 gctatgccag caattccagc ggcggctgct gcggcgtatc ccttccaccg gaagaacagc
     3841 gccagctcaa acgcttgaag gaaggggccc aggccgaaaa gaaagattga caggggctgt
     3901 aaaaacagct acactaagga tataagaaca agacgcacga cggggacacc acctaaggtg
     3961 tcagacgtcg tgcgttttta ttttgttacg aggggtgtat accatgcagt tagtattgaa
     4021 tggaaagaaa atgaattgtc cctgtgacca cctggaagac ctgaaagccg cctacggcag
     4081 cggtcaggaa atcatcatcg tcaacggctt tgccacgaca gagaacttag ccttgaaaga
     4141 gggcgatgaa atctatttca tccccaaaga ccgcctgccg ccgaaagaag ccctggaagg
     4201 catgatgtgt tcccgccata cgccgaaagt ccaccagaaa gtcagcgccg gccgcgtcgc
     4261 catctgcggc ctcggcggcc tggggtccaa cgccgccgtc tacctggccc ggaccggtgt
     4321 cggccatctg cacctcatcg acttcgatac cgtcgatgcc agcaacctca accgccagtc
     4381 ctacatggtc cgcgacctgg gccagcgcaa gaccgacgcc ctggcccggc agattgccga
     4441 catcaatccc ttcatcgacg tccgcacgga cttcgtccgc ctcactgaag acaacgtgct
     4501 gaccatcctg aaagacgacc aggtcatctg cgaagccttc gacaaccccg atgccaagac
     4561 catgctcgtc cacaccatcc tggaacaatg tcccgataaa tacatcgtct ccgcctcagg
     4621 catggccggc tacggcgaca gcaacgccat cgaaacaaaa aaaatcacca gccacttcta
     4681 catctgcggc gaccagacaa ggagcgccaa acccggccgc ggcctcatgg cccctagagt
     4741 cgccatctgc gccggccacg aagccaattt gatcctaaaa ctcctggtcg acgtcttata
     4801 aatgcctctc ctcataggag aggtggcccg caaggccgga gaggtttata aatttttaga
     4861 gcccatgcat tatcatgtgt gggctctttt gctgtatggc tgggttagat tctagaaaat
     4921 aagctataga agactaggag ctcaatgaaa gggagctggc tacccaaggc ggtctgagaa
     4981 attctttgtg agagaagctg attatatatg gcacattgat tgaatgaata gtttagccca
     5041 attatgatgt gtttatattt tgttaaggtg tttgtgtagt attattcttt tgtaataaca
     5101 ttcttaaaga ttgagtttct actattctat agttcgataa ccatagctgt ttgtctttgc
     5161 ctgcaagttg agggttcgat tgagtttcaa atgtattttg agcaaggctt tctccaacat
     5221 ttttccacat ttggataggg ttaaaaatcc aatttacagt tcctttatct ttatcgaatc
     5281 gaagaagaca gcgaaaagca ccaaaaatcg gatagatgaa tcctgctgag atattataag
     5341 tttgatcata gtgcaaaaaa gatgttttta tagtcttgcc gttttgcagg gattctactc
     5401 cttttacact gccaaacctt gaatttttaa tgcctgcttt ttctttgtat gccttataaa
     5461 atgaaggtag ctcatattca attaagtcgt ataagtttgt caagactgga atcttttttg
     5521 taagtgtttt atagaattgg tttgcgaatg cttctttata acgtttaaat acctgtgctt
     5581 tgccagaata actttgaacg ggggctgttg aatcatctgg atatttttca atatcaaaag
     5641 cgtacattaa tcgtaataat tcagaaacat tgatatcttt attttcattg tccttatagg
     5701 caatattatt ggaataaagt tcatttgaaa ttgcagattt tacatctaca aagttatcat
     5761 caagattgaa taatgcaata tcagagactt gtacagatgt atttcttgca tcagaaagtc
     5821 ttgtaatatt ttgcacatta gtaataactt ctaatctgac atatttttca atatcggggg
     5881 ggatagcttt tctttttttc atgattgctg tgtatgtatg tcctccatct aaaatgccat
     5941 aagaatattt atcgtcatca agttcctggt ctcccagatc aatagtgact tctgattttt
     6001 gactattaaa tacaagtgac tttgcagcaa taacaatacc tcgattattg atataaaagt
     6061 ctgtttctgg ttcgactacc gcattgagta aatttttggc aacatgagta gtcattttag
     6121 gttttcttgg atttacatcc aatggaatat tgttaggaag gtctttcata tttactaaca
     6181 agtaataaat tgtttcaaat tggccagttt ctcctctctt ttgataagga gaaggtaatg
     6241 ttctaatcat atttactcta aaattgataa tttctttcat taattaggac tcccttcaat
     6301 ttaatggcta aaattcttga attaattgca acattattgt atattatata taaaaataaa
     6361 acaatgagcc atggtgccta atttatggag atgaataatg gtagaaagaa aacaagatga
     6421 tcttagatta gtaaaaattg gtcaattact attagaaaaa agaaaattat tagggaaaaa
     6481 ttattcctca agagaaaaat ttatatatag tagaagtaaa gaattatttg gcggaaaaga
     6541 ctggatatca attcgacatt tgactaattt agaacttgga aagaatttac caagtattaa
     6601 tatgcttatt acattatcct atgcttatga aatagatccc atagaacttt ttagtgaaat
     6661 tcttgctgtt tatgaaatgg aggaataaag tgaaaaggtg gagtgggttt ggaagaatat
     6721 tggcgaatac ctaaaaatag aatggtgtaa aataattagg gggaacttta cgttgcctag
     6781 taagttgata caaggcctga actaagtctg ctgaattttt tacggttttg gccttagaga
     6841 ctaaaatacc aaaattttca cgctgtattt gccaacttat atggcttcac agcttttgcg
     6901 gactggcatt ttggtacttt tagtagtgtt agtcagtgct tttcttcctt ttcaatctgg
     6961 gacaacaaat aaaactatag tatgtagtgt acaggattcg cggcggcctt tttggcccgt
     7021 cgggtgccga ctagaggagg cgcaggatgg gtgccaaaac ggacagcagt ggctggacgc
     7081 cctgggtcgg ggcgtcccct acgataaccc cgattggggc ttttctttac gagctgaagg
     7141 tcacccgaag cgaacggcga ccggcgaccg gcgggccgat aggctccaat ctacattaat
     7201 ggttccttaa atattttgga tttggttcaa aaatgattag aaaaggatta agttttccag
     7261 aaccgtcctt tttctgttgt atcatggagg tatggatata tc
//
LOCUS       DAWCYS010000062         7086 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_656_length_7086_cov_5.548713, whole genome shotgun sequence.
ACCESSION   DAWCYS010000062 DAWCYS010000000
VERSION     DAWCYS010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_7086_cov_5.548713"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1786 a         2061 c         1804 g         1435 t
ORIGIN      
        1 gattaccaca tcatcgactg cgtcctggct tcggatacgc tcaacgctta tgaagaagcc
       61 cgccgtgccc aggccctcat cgatgccgac atcatgggca agaccgacct cttgcaggcc
      121 ttcacccgcg tcagcaacat gatcaaaggc gccgacgata ccgacgtcaa tccggacctc
      181 tttgaaacgg aagaagaaaa ggccctttac agcgcctgcc aggccatgca ggtcaacttg
      241 ccgcagaaat acgccgtcta tgactacgaa ggcgtagccg aagtcctctc cgaaggcatt
      301 gccgccatca atgacttcct ggacaacgtc ctggtcatgc acaaagacga agcggtcaaa
      361 gaaaaccgtc tccacctctt ggccctgacc tacagcctca tccagccctt gggcgacatc
      421 aagaaattgg cgtaggggcc gttccgacgg acggtccgca ggccgctttg gaatcagatg
      481 ccgctgcgcg gcgacagact caggtggaca acgacgcttc gggacaccct cggcgcgtaa
      541 aaagcctccc ctgagaaggg gagggggacc gcttgcggtg gaggggttct ttacgacata
      601 cccttagcgt attgtagaga aagaacccct cagtcagcag agctgccagc tcccctaata
      661 tgggagccta agcctcccat attaggggag gtggcccaac gggccggaga ggttgattgt
      721 actttcgcgg ctcgccgcga atcccgtgga taaagcggaa attcaatggc caaaaatgaa
      781 aaatatgata taatgaatag tgtccaagtt tgtagtgtag agtttgtagt ttgtagatga
      841 caggattgaa tttgagttat ttactgtaaa ctaaaaacta caaacttcaa actcgaaaag
      901 aggctgcctt cgtgtgacag cctcttttcc gcatctacca taaaggagtg atcatttatg
      961 aaattgaaga atctcgtcat gttggcagca gcccttacct gtattgccgg ttcgaccgtc
     1021 atggctgaag aaaaggtggg cctcaagagc gccagtgccg acagctatga agtcgtccag
     1081 gtcatgacga ctaaagacaa gaattactat cagtactata acagcgccta tcagtatgcc
     1141 gtcgatatcc cccgggctgc gacgacagcc gacatgaatg ccgacggcga tggctgctat
     1201 ttccaggatc ccaaagacga tgccgtctac atgacctatg ccgccaagaa caccatgggc
     1261 ttcactatcg acgaactcta caacatggct ttgggcatga acggatcgcc gacactgacg
     1321 accaacatca agaccaagaa cagctatgcc atcggctgga cggacggcaa gaagtcttac
     1381 taccacgaac tctacatcaa cgacaagaac aagacctaca cggccttcac cgtcacctat
     1441 ccgaccagca aaaaagataa gtaccaggcc atcatcgccc acatgtcccg cagcttcatg
     1501 cccaacgtac aaatgtaatg ataggggatg cccaatgggc atcccgccgg tcgcagttcg
     1561 caggccgcag gccgcctcgg aatcagatgc ggcgtcccgc cgcgacagac tcaggtggac
     1621 aacatcgcct cgagtaaccc tcggctcgta aaaaatgccc cggctggggc cttcgtaggg
     1681 gccgtcctaa gcctttttgg cccgtcaggt gccgaccaac gagaggcgca agacggttgc
     1741 caaaacggac agctgtggct aggcggcccg ccgtgaatcc tgtgaatgca ggggaggtgg
     1801 cccgaagggc cggagaggtt gctgcgcttt caagcagccc tttacggaag atgcctataa
     1861 aatggaaaaa tcccccttta caggaccgcg gctttatggt ataattccct tgttaattta
     1921 ggcagtcctc tgctcgatac acgattgtcg tgaaaggtgg cggcaacggg ggcacgaatg
     1981 tcttatagga ggaaaaaaga aatgaacatt atcagcgtac ttgaacagga acagcttcgt
     2041 gacgatatcc cggcattccg ccctggtgat acagtcaaag ttcacgtaaa ggttgtcgaa
     2101 ggcaatcgcg aacgtgtaca gatttttgaa ggtgtcgtta ttggccgtca gaacggcggt
     2161 gtccgcgaaa ctttcactgt ccgccgcgtt tcctacggta ttggtgttga acgtactttc
     2221 ttggtacatt ccccgcgcct cgctaaaatc gaagtcgttc gccacggtgt cgtccgtcgt
     2281 gcaaaactgc actacctccg tggtcttaca ggcaaagctg cccgtatccg cgaacgtcgg
     2341 taattcattg gcaagaacac ttgaaaaaga ggctgcccac gaagcatggc ttcgcgggca
     2401 gcctcttttt tcatgactcc atcgtagggg tcgtccctaa gggtggccaa aggcaacctc
     2461 tcagtcagct gcgctgacag ctcccctata aagggagcca aagcctctcc tcataggaga
     2521 ggtggcccga agggccggag aggttggtct taaacaaatg ggccgtcctg cgattcagga
     2581 tatcgtgtac tcatattgtt aaccttgata caaacgaaat aaagttgact gcaatcgccg
     2641 cagcgagtat aataatccca tagttcacaa taaaaatagg gagtaggttg acaatgaata
     2701 cgatagcagc actgaccgat agcattatac aggggtaccg catccaacgc ggcgatgatt
     2761 tatcgatttt cctggaagca cctttggaag aattgcagca gggagccggc gttttacagc
     2821 gccatttttg tggcaatcat attgatttct gcaccatcat caacggccgc agcggccgct
     2881 gtggggaaga ctgcaaatac tgtgcccagt cggcctgcca ccatacgggc tgcgaagaat
     2941 acggtttcct ggcgaaagaa gaaatcctgg ccaacgccaa ggccaatcag gatgccggcg
     3001 ccaaccgctt tgccatcgtc acgtcgggcc gggctctgac aggggcggat tttgaaaaag
     3061 ccctggatac gtaccgcacg atgaagcaga cgctgcacat cgacctctgt gcatcgcacg
     3121 gcctgctgac gccggaacag ctgcaccgcc tccacgaaac gggcgtgacc agttatcacc
     3181 ataatatcga gacgtcgcgg cgtttcttcc catacatctg cacgacccac acctatgacg
     3241 accgcatccg caccatcaag gccgctcaga aagaagggct ctgcgtctgt tccggcggca
     3301 tcatcggcat gggggaaacc tgggaagacc gcctggacat ggccatcagc ttggccgaac
     3361 tgggcattca gtccattccc gtcaacgccc tcatgcccat tcccggcacg cccctggaac
     3421 atcggcccca gctcgacggc cgcgatgtcc tgcggaccat cgccttcttc cgcttcatca
     3481 atcctgaagc taacatccgc ctggctgccg gccgcaaact cctgccggaa aacggcgcca
     3541 cggccctgct ccacggcgcg tcggcctcga ttacgggcaa catgctgacc acgtcgggct
     3601 cgaccatcgc cgaagacatg gccatgctcc agcgcctggg cttgactaat tcgtaggtcg
     3661 taggccgtag atcgttcgtc gttgatggta cgattaaaaa tcataactaa tacgacgaac
     3721 gatgaacgaa aaacgtttga acctgcgagg gctatgtgca tgatagtcta tactggtgta
     3781 tgacaagccc tgcactagcc ttcatccttt gtctatgata taatgttagg gatatagaaa
     3841 catacatata cttttcaatg aggtgattat cgtggcaaaa cagatttttg ttaccggtca
     3901 taagagcccg gacacggact ccatttgttc ttccattgcc tatgcaaaat taaaacagtt
     3961 acagggtatc gacgctacgc cgatccgcgc tggcgaaccg aataaggaaa cggcctttgc
     4021 cctgaattat ttccacgttg ctccgcagcc actggtcaag gacttctacg tcaaagtcag
     4081 cgaagctgtc cacacggaag tcgaagccct tccggtcagt gccagcctga aagatgctgc
     4141 caaatggttc gcatcccacg aaagcgatgt cgctcccgtt gccgataacg gccagttcgt
     4201 cggcgtcatc gaaaaaggcc agctcgccga tgaattcgtc aagaccgtca tgggtgaaga
     4261 attgagcacg gtcaaagcct tggctcacga tccggaattg ttcttcaagc cgaccgataa
     4321 cggccatgat atcccggctg atggtaaata cgctgtcgtc gaaaacggca aatacgtcgg
     4381 catgatctgt gcagactgtg tcgctgctgt cgaaaaacag caggtcatcc tcgtcgacca
     4441 caacgaaaag aaacagatca tcgacggcat cgacgaagca tctatcgtcg gcgtcaacga
     4501 ccctccccgc atcggcggcc ctttggaaac agccggccct atttccatcc tcttcaaacc
     4561 cgtcggctgc acggcaacca tcgtaacggg cttgtatgaa caggctaagg tcgacatccc
     4621 caaagacatt gccggcctgc tcttgtcggc cgtcatttcc gatacggtcc tcttccgttc
     4681 gccgacctgc acgccggctg accgcgaagc tgccgaaaaa ttggccaaca tcgctggcgt
     4741 agacgtcgaa aaatacggca tggaaatgct caacgccggt gccaacgtct ccgatttgcc
     4801 gccggcccag atcgtccaca acgacatgaa agaattctcc agcggcaata cgaccttcac
     4861 cgttgcccag gtccaggtca tggatacgac ggacctcctc gaccagaaac aggtcctgct
     4921 caacgccttg gaaaaattgc gcgctgaaca cgattacgcc gcttccttcc tgatgatcac
     4981 cagcatcatc gacgaatcga cgaacctcat cttcgccggt aatatggacg acgtcgtcag
     5041 cagtgccttc ggcaaggacg tcctcgataa ggaagtctac ctgcccaagg tcatgagccg
     5101 taagaaacag gtcattccgc ctatcctttc ggcaatgaaa taagtgatga ttttcagagg
     5161 ccccatcgac acggtgtggg cctttttatc tataaagggg aagaatgcat gaatcctatt
     5221 tttgataccc tcaacgaccg ccagtgcgag gccgtcaagc atactgaagg gccgctgctg
     5281 atcacggccg gtgccggatc gggcaagacc aaagtcctga cctgccgcat tgcccatctc
     5341 ctggaattag gcgtcgcgcc gtaccgcatc ctggccatta cctttaccaa caaggccgcc
     5401 aaggaaatga aggaacgtgt gaccaacctc gtcggtgccc aggccgacag catctggctg
     5461 agtacgtttc actcgttctg cgccaagctg ctgcgctttg aaatcgacgg cttccacggc
     5521 tatacgcgca atttcaccat ctacgattcg tcggaccagt tggtcctggt caaggactgc
     5581 ctgaaaaaac tcaacctcga cgacaagcag ttcacgccgc gctccgtgtt ggggaccatt
     5641 tcatcggcta agaacgtcct catggatgcc aaggcctttg cggcgaaagc ctccgatttc
     5701 tacgaacaga aagtcgccga cgtctacgct ctgtatcagg aaaaattgcg ggaaaacaat
     5761 gccgtcgatt tcgacgacct tttgttcctg gccgtgcgcc tcttgcagga aaacgaggaa
     5821 gtccgggaaa aataccagtc ccggttccaa tatatcctgg tcgacgaata tcaggatacg
     5881 aaccatgccc agtatgccct gaccaagata ctggccgccc gctggcgcaa tatctgcgtc
     5941 gtcggcgatg ccgaccagag tatttacgcc tggcgcggtg ccgatatccg caacatcatc
     6001 gacttcacgc gggactatcc cgatgcggcg tcgatcaaac tggaacagaa ctaccgctcg
     6061 acgaagacta tcctacatgc ggccaatgca gtcatcgaca ataacgaaag ccgtcccaag
     6121 aagaccctgt ggacagataa cccgacgggg aacaagatca tccattacca ggcccagacg
     6181 gaacacgacg aagccgatta tatcgccggc gtcatctaca accgccatga aatcagccat
     6241 gaaccgtatg gcgacatggc catcctcttc cggaccaacg cccagtcccg tgtcctcgaa
     6301 gaaaagctca tgcgctatgc cattccctat accatggtcg gcggcaccaa gttctatgac
     6361 cgcaaggaaa tcaaagacgt tctggcctac ctgagcctgc tctataaccc ggaagacagc
     6421 ctgagcctga cgcgcatcat caacgtgccc aagcgcaaca tcggtgcgac gacgatggaa
     6481 cacgtcgcgg cctatgccga agcacagggg atttccttat ttgaagccct gtcgtcgacc
     6541 gatgagatcc ccgtcaccaa acgggccaag gcatcgctgg aaaacttcgc tgccatgatt
     6601 ttcgacctcc tcaacgacat cgaaggcaaa gacgtcctga gcctcattga aacggtcatc
     6661 aaacagaccg gttacggcga catgctcgat aaagaagcgg aacacgaccc gcagggcgaa
     6721 agccgcaagg aaaacgtcgg cgaattcctg tccgtcgcca aagactacat ggacagcaat
     6781 cccgaaggca acctccagga cttcctggaa aacgtcgccc tcgtcagcga tgtcgatgat
     6841 tttgaaagca gcgactcgaa agtcacactc atgaccctcc atgccgccaa gggcctggaa
     6901 ttcccggtcg tcttcctgac cggcctcgac gaaggcctgt tcccgcacag ccggaccctg
     6961 atggacccag cgcaggtcga agaagaacgg cgtctggcct atgtcggcat taccgggtcc
     7021 gaacggcagc tctacgtgac cactgccttc acgcggacga tgtacggccg catctaggcc
     7081 tatatg
//
LOCUS       DAWCYS010000063         7067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_659_length_7067_cov_6.668853, whole genome shotgun sequence.
ACCESSION   DAWCYS010000063 DAWCYS010000000
VERSION     DAWCYS010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_7067_cov_6.668853"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1589 a         1792 c         1960 g         1726 t
ORIGIN      
        1 acacagggcc ggtcaaagcc gtctgccaca gcggacgctg gcggacattg tccatcgtcc
       61 cttcgacgag gggccgcagc gcctgggatg cagcgccggc actgccggtc cagcggctta
      121 tgagcttctg ggcaatggcc agggctgtca cgacgtagtt ggagcagaag caggccgcag
      181 catgatagag catgcggtct tcagggggta cgaaaaaagg cgtactgccc aaagtccgga
      241 ccagatctgc cgcaacggcc ttggcccgtt catcaccatc gacggccatg tagattccct
      301 tcaggcggtc tgccgaaggt tcggcaaagc tttgcagcgg atgaaggctg ccgacaggat
      361 accccaaccg tgaaaggggc tcaagaggcg ataaatccat agctccactg cagtgaaata
      421 tacaggtctg aggcggcagc gcatcccctg tcagggaagc ggccaattcc cggctaacgg
      481 gagccagggc atcgtcgcgt actgtgagca ggaggacatc gcagtgccgt gcgagttcag
      541 caccgtcggt ataggcagca gcatggaggc ggcgggccat ttgacgtgta tgttcttctg
      601 tggaacaaag gattccttcg acatcggaag gaaaagctaa tacaaaagaa gcgccgactc
      661 tgccggcgcc gataatacct aaacgcatga taactccctc aaaaaaaagc gcattcctct
      721 cgggaatgcg cgtaaaaagc ctacgtttac aagaattcgt cccggtcttt cgatccaagc
      781 gatatgctat gaaattatga taatagttga cccggatttt ttacggtacg gatcccaaaa
      841 gattccgtcc aacccgaatc acatgtacag gatactacat ttgtgaaact tttaccaata
      901 acttttaaga atagagtatg atagtggttt gtggctcgtg gctcgtggct catggctcat
      961 ggctcgtggt tcgtagctag tggctcgtaa aagcactaag tattagccac gaacaactag
     1021 ccactagcca caaaaaagag ctgtctataa agacagctct tttttcacac acaatatggt
     1081 gggcggtaat ggattcgaac catcgacccc ctcgatgtca acgagatact ctaaccaact
     1141 gagctaaccg cccatatttt gtacttctct gtgcgacttg tttattataa cttacttttc
     1201 cctatttggc aaggggtatg ggaaaattat ttcagcattt ttttgatttc gtatgtcacc
     1261 tggcgctcat tcaatacggt attaaagggg cgcgtcatct ggccgccgca ggcgatgaag
     1321 ataagctgcg gtacctgatg gacgccatag tcatggagca tctgtttctg tgtgctgtat
     1381 acgggcaggg catatccctg ctggtggata taggcccggc tatctgctgt atccggtccg
     1441 acggggatgg tcacgaaata gacatcctgg ccgtactgga cacaggcctt ttccaggatg
     1501 gccagctggc gcaggctgtc gctgctccag gatgcccaga aaaagaggca gaccggcttt
     1561 tggttataca gagcctgcaa gcgtatgtcc cttccatcgg gagcctgcag gacctcatca
     1621 ggtgccagca tttccgtttc ccgctcgctc acgtcttccg cttccctgac gacgtcggaa
     1681 ctataggcat cttcttcttt gccaggccag aaaaagactg tcagtatcag acagatacag
     1741 gccaggccgg ccaggatttt cgtcttgatg ttcatcatct ctccccccct ctttttctat
     1801 ccattataac tgaaaaaagt ggttcgtggc taggggctaa caacgtgaag agggcttgtc
     1861 gtcgtacgac aagccctcga gtctggggat aggatctatt gtaattacgt attatgcgtg
     1921 gatgtagatg cggacggccc ggttgatgag gttgatcttg cgtccgtaga aaatgtagta
     1981 catggcaaag caggcaatgg ctacgatagc agcgacggca tagagctggg ggaacgtgag
     2041 caggccgtgg aggctgccga ggacatacgg gcctacgccg atgccaaggt cgagggcgac
     2101 gaagtacgtc gacagggcga cgctgatgcg gtacgacgga gtcagtttga tgcagactgc
     2161 ctgaccgttg gacatgaacg tgccatagcc gaggccgacg aagacgcccg ataagagaag
     2221 ctgccacgaa gcaccggtga tgctgaggag gaacaagccg atggccaggc agatgtagca
     2281 gggatagagg acgtaatttt cgcctttatg gtcgaagatg acgcccgtca tcggacgggt
     2341 gagggggatg acgaccgcat agagcacgaa gacgaacggg ccggcctgga ccaggttgat
     2401 ttcacgggca taagcggcca ggaaagtcag gacgctggaa taagcgaagc ccatgaagaa
     2461 gccgatgatg gagatgacca tgacgcgata ttcgacgtaa ttgtcgaggc tcatggcttt
     2521 catcttgccc acctgttcag acgtgagggt aatttcgtcg atgtggaggg cgatgcagcc
     2581 aatcatgcac aagccgacga ggatggcgca gagggtgatg ataaaggtga agttcgtgac
     2641 gacgatgagg aacatgccca ggaaaggtcc gacggcagcg gccaggctag tgctcaggcc
     2701 gtagtagttg atgccttcac cccgcttatc ggcaggaata caggaagcga caatcgtgtt
     2761 cgtcgctgtc gagacgatgc cataggccat gccgttgagg aagcggatag caaagagaat
     2821 agcgagattg ggaatataaa aatataataa cgtcgttaca aaataaagga tgatcccgcc
     2881 atataacgat ttgcggcggc catacaattc aatagctttg ccgaactgca agcgggccag
     2941 caaagtgccg acgatgaaga tgcccgaagc caggccggct tcaccgagag aagcatgaag
     3001 ctggtctttg gcgacgacgg cgataataac catcaataaa taataaatga gatagactac
     3061 aaaattgata cctgtgtcga gaaggaagtc tttgttccaa agtgattgat tcgacattct
     3121 gtattcactg ctcctttcgt atttgcaagc tagattctac accttctctc ctatcttgta
     3181 taacgcaaaa aaaagatacc tgtcatcgtt ttttacgatg gcagatacct ttatcattat
     3241 tttttgatat gatacacgcg acggatatag tcgacgaaga cctgtatcgt cgcgtacttg
     3301 ggattgtcct tcttatagac cagataacag ggcaagcgca gcggcggttc caatgcacag
     3361 tagccctggg tgatgcccat ggcctggagg aatcgttccg gcaggatagc tccgatgcgg
     3421 ttctgctggc aatagttcat catggcatag ggcgtagaga accaggcctg cgtttcctga
     3481 ggcgggcagg catgggccgt gaaatagtga ttgatgtagt tattcatttc gtattgcggc
     3541 gaatattgcg ccaacggctg ggatgccacg tattgaggcg tcacggcatg ttccggcaga
     3601 tccaggttcg gcccgtaata gacgaggttc tcgatgccaa aaaagacctt ctcgtagtct
     3661 tccaggtgca gtgccggcag ataaaagagg gacacgatat ccagtttatc gtctttcagc
     3721 cattggatca tctcccggac ttccaggttg tagacccgga aagaagcgcc gctgtggtgc
     3781 tcctggaaat aagcgaggtg gcggttcatg tggacgtcgc ctaaggacag catgattccc
     3841 accgacaggg gctgggtctt ccctgtccgc cctggcagtt cgctcatggc ttcttctaat
     3901 tcacctaaaa tgcgggctat tttcgtgtag aacatcttgc cagccggcgt caagtgactt
     3961 cccctgttcg agcgctcgat gaggcggacg ccgacgaggt cttccagttt cttcatctgc
     4021 gcactgaaag ccgactgggt aatgttggcg gcattggcag ccgtcgtgaa atttccatac
     4081 ttcccgtaaa tgacgaaatt ccgcagctgc tgtatcgacg gtaaatccat gggacctcct
     4141 tttttcgttc ctcgtaggtc gttcctcgta ggtcgaaaaa aagcatccca tcacgatgaa
     4201 cgacgaacga tgaacgataa agcaaaaagg ccttgcatga tggccgaaac cttcatgcag
     4261 ggccccttca attacttatc taagagttca tcgaattttg cattggctgc gtcgaactct
     4321 tcgtccgtcg gtgcgacgta gacgtcttcg ccgtcttcct ggtcgacgcg ggcgatgatg
     4381 gcatcgccgc cttcttcgtc ttccgtgact tcgacgagca gggtgaatac tttaccatcg
     4441 aggtcgaggc gctgtacttc ctgatattcc acttcgttgc cttggtcatc cgtagcggtc
     4501 acgataatga cttcttccaa ttcgttgttc atttcttccg acatagtagt cctccttaat
     4561 gagcatgagc gtctaaataa ttctgtaaaa taaggattgc tgccatttta tcgacaaact
     4621 gcttgcgctt ttcgcgccgc acattgcctt cctttaatac attctgggcc ataacggtcg
     4681 tcaggcgttc atcccaatag acaatgtcta tatccgggac ggtttgctgg aatcgttcga
     4741 cgaattcttc gatttttttc atattatcgc tgtagtcacc gttcatatga cgcggcttgc
     4801 ctacgacgat gcggtgtact tctttttctt tgatcagttc gcgcagacgg gcaaagtctg
     4861 cttcatcgga tttacggcgg atcgtttcta cgccctgggc cgtaatcagc aaggcatcgc
     4921 tgcaggcgac accgatggtc ttggaaccga catcgagtcc taaaattctc atatgtctcc
     4981 tataagttgt gctgtttgac gtacgaacgg actaattctt cgatgagttc atctcgttcg
     5041 atgcgccgga tattgctgcg agcattgttg tggctcgtga tatacgtcgg gtcaccggac
     5101 aggagatagc ctacgatctg attaatcggg ttgtatcctt tttcttccaa tgcgtgatat
     5161 acatcttcca gaatcatgga tgcaacggtc tgttcttcac ttcggttttg cataatcatc
     5221 gtttcgtcgc ttactgccat gttcactcac atccttttta ttcaactggc atgactgcca
     5281 aaaatttatg ctaacatatc gcgtacggct gcggcaccgg ctttaagggc atcgcccaat
     5341 ttttccggtt ccttgccgcc ggcctgggcc atatcgggac ggccgccgcc attgccgcca
     5401 gctgcctgag cagcagcttt gatgatcttg ccggcatgga ggcctttctt ggtatcgctc
     5461 ttggcgactt tgcagacgaa gttgacttta tcgccattgg tcatccccaa gaggacgacg
     5521 ccgctgccga gcttgtcgag gaccgtatca gccaggtcgc gcaggttatc catagaatca
     5581 gcctgagcga cggctgcgac gacaggaacg ccgccgatat cttctttagc tgccaggatg
     5641 ctgtccatat cggacagggc tgcgtctttc ttgagctgag ccaattcttt ttccgtatcc
     5701 ttgagctggg ccaggagagc ttccagtttg gccggcgcat cgctgacctt gcctttcaag
     5761 aggcctgcca gctgctgcag ttcctgcgat tcatccttag ccagcttgag ggcagccgga
     5821 ccggtaacgg cttcgatacg gcggacgccg gagccagtcc cggtttcgct ggtaatgcgg
     5881 aacgtaccga tgaccgacgt gttggccaca tgagagccgc cgcagagttc gcagctgaag
     5941 tcgtcgacgg agacgacgcg gacgatatcg ccgtattttt caccgaacaa ggccatagcg
     6001 cccttttctt tggccgcatc gagggccatt tcttcgatgg tgacggggac ggccttgagg
     6061 atttcttcgt tgaccagggc ttcgatctta tccagttcat cctgcgtgac cggcgagaaa
     6121 tgggtgaagt cgaaacgcag gtggtccggt gtgaccagtg agccggcctg gttgacgtga
     6181 ttgcccagga ctttgcgcag ggcagcctgc aagaggtgtg ttgccgtatg gttgcgggcc
     6241 aggttcatat gacgctgctt gtcgacagcg atatcgacgg catcgtccgt gcggaggacg
     6301 ccttcagtga tttcagcgat gaggtatacc aggccattgg gcagagcctg ggcatcagtg
     6361 acctgtgcct tgccttcagc agcacagagg acgcctgtat cgctgagctg accgccgcct
     6421 tcggcatgga aggggttgtc gtcgaggata atcgtcactt cctggccgtc gcgggcttcg
     6481 ctgatttcga cgccttcctt gccgagcatg gcaatcgtcg ctttgacagc cgtttcgtcg
     6541 ttgaccagtt tcgactggtc cagcttggtc gtatccggcg tagcgacttt ggccgatact
     6601 ttggtatggg cagcgcgggc ccgttcctgc tgttccttca tggcttcctg gaagccgtct
     6661 ttatcgactt tgaagccctg ttcgccggcg atttcttcgg tcagttccat ggggaagcca
     6721 tacgtatcgt agagtttgaa gacttcagcg ccagtgagga ctttgtcgtc gcccacatgg
     6781 gccatcatat cgttgagcag ttccatgccc tgtgccaggg tagcgctgaa ctgtttttct
     6841 tctgtattga tgatctgttt gatgagggct tcgtgttccc gcgtttccgg atattcatcg
     6901 ccgaggatgt ccaataccgt atcgacgaga cctgccagga agaggtcctt gatgcccagg
     6961 aggcggccga agcggacggc acggcgcagg atacggcgca ggacgtagcc gctgccttca
     7021 ttggacggca ggattacatc ggaaatcatg aaggtaatgg cgcgtat
//
LOCUS       DAWCYS010000064         7031 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_663_length_7031_cov_5.275516, whole genome shotgun sequence.
ACCESSION   DAWCYS010000064 DAWCYS010000000
VERSION     DAWCYS010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_663_length_7031_cov_5.275516"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1369 a         1861 c         2198 g         1603 t
ORIGIN      
        1 ctatcttgac gaaggcatgg tggccgtttt tcttgacctg gtcggggata tagaacgggg
       61 tctgctcatt atcaagctgc gtcaccaggg ccgccgtctg gctgaccaaa tcgttgaagt
      121 tggctgccag cagattgacc tggatgacca gcggcttgac gatgatattg gtaataatcg
      181 tcacgatgac gatgaggatg acaaaggaga tgaggatggc aaggtcgatg ggtacatgac
      241 ggccggtcag gcgccgcagc cctttgatga cgccatcgcg gataggcatg aggatgaacg
      301 tcaggatcag ggaaatgacg acgggccagt agacggcatg gaccgtgagg aacagcgcac
      361 cggccaggat ggccagggtg acgcgcatcc agaactccgc gcccttatag gcaaccatgg
      421 tttaccagcc tccgtccagg aatggattgc cacgccgttc gtcgccgatg gacgtagccg
      481 ggccgtggcc aggcaggacc agcgtattgt caggcagggt catgagtttc gtttccaggc
      541 tgtggagcag cgtcttcgac gagccgccgt agaggtcggt acggccgacg gaatagcgga
      601 acagcgtgtc gccggaaaag acgacgcctt caccgtacag gctgaggccg cccggcgtat
      661 ggcccggcgt ttccaggact gtgaagtgca gatgccccaa tgtcagttca tcgccgtcct
      721 tgaccaggtg gtctgccgac gtacattcga tggcctggcc catgaacagg gacaggttat
      781 tgtgcgaatt ggaaatgagg tcggcatcgc cggcgccgac gtagaccggt acgttcttgc
      841 gctggcgcag ttcctgcagg gcgccgatgt ggtcagcatg gccatgggtc aggaagatga
      901 acttcagcgt caggccatgg ctggtcagag ccttgtcgat ggcgggataa tcccaggccg
      961 gatcgacgac catggcttct ttcgtatccg tgtcgtagac gatataacaa ttggtcatga
     1021 taggaccgag ttccatactg atatatttca tgctcataag tatacttccc ccttaaaagg
     1081 ttttctggct gtccaagagg atggtcacag gcccgtcgtt tgccagggat acgaccatat
     1141 gggtccggaa atggcctgtc gccacggtga cgccgaaggc gcggacagcg gcgacgaact
     1201 gctcatataa tgcttcggcc tgttcgggct tggccgcttc gatatagctg ggacggcggc
     1261 cgtggcgggc atcgccgtac agggtgaact gggaaatgac cagcatctcc cctccctcgt
     1321 cgaggaggga gtgattgagt ttgccctggt catcttcaaa gatacgcaag tggacgatct
     1381 tgtcggccag gtagcggacg tcagcttccg tatccttgac ggaaacgccg aggaggaccg
     1441 tcaggccgtt gcctgcctgg ccggtgagga cgccgtccga ggtcacagaa gacgtcaggg
     1501 tcttttgtac gacagctctc atgtctggct ccctcccgta tagcgttcga cggcgtagac
     1561 atccttcagg cggcgtatct tggtggccac gaattcaaac tgggccaggt ccttgatgct
     1621 gatgcccatg tggatggtga cgttcttggt atcgctggtc ttggcgttga cgctgaggat
     1681 ggaaatcttc atttccgcca gggtggccat gatttcggcc atgatgcccg tgcggtcgta
     1741 ggcgacgatt tccatgttca cttcaaagcg ttcgttgccg tcgtaatccc attcgacgtc
     1801 gatgaggcgg tctacgtcgt tctggccgtg cgtgacgttc gggcagtccg cgcggtggac
     1861 cgagacgccg cggccgcggg tgatgaagcc gatgatggga tcgcccggta cagggctgca
     1921 gcatttggcc aggcggacca gcaggcccgg ttcgcctttg accaggatgc ccgtgccgtt
     1981 gtgcttgacg ggtttcttgg tcttgagttt ttccagggca gccaggctgt tggtcttttc
     2041 ttcctgcttc tgcaagtctt tcttgtgcag ttccaggagc ttgatgagga ccgtattgac
     2101 ggcaaagccg ccgtagccga cagcggcgac caggtcgtct tcggagccgg cgttcatctg
     2161 cttggccacg cggcccaggc ggccgccgac gttgagggct ttccagtcgt ggcccaggcg
     2221 cttacattcc cgttcgagcg catccaggcc cttggcgatg ttttcttcgc ggttttcctt
     2281 cttgaaccag ctgcgtatct tcgagcggct ttcggaggtg ccgacgatgt tcagccagtc
     2341 caggctgggc ttgccgactt tggacgtaat gatttcgacg atgtcgccgt tcttcaattt
     2401 atagtccaaa gggacgatct tgccgttgat cttggcgccg acgcaacggt ggccgacgtc
     2461 ggtgtggatg cggtaggcaa agtcgatggg gatagaaccc tggggcaggt cgatgacgtc
     2521 gccccgcggc gaaaaaacga agacttcgtc ggaaaaggcg tcgagcttca gggcgttgac
     2581 gaattctttc ggattgctcg tgtcctgcca ttccaggagg ttgcgcagcc agcccatttt
     2641 ttcatcgaaa gcgtccttgt tggccgtctg gttgccttct ttataacgcc aatgggcggc
     2701 gacgccgtat tcggcgatgc ggtgcatttc ccaggtgcgg atctggattt ctacaggctg
     2761 ccctctcgtc ccgatgactg tcgtgtgcag ggactggtag ttattgggct taggcatggc
     2821 gatgtagtcc ttgaagcggt acggcagtgg cttccacagg ctgtggacga tacccaggac
     2881 gccgtagcag tccttgacgt cgtcgacgat gacgcggatg gcgaagaggt catagacctg
     2941 gctcaagtcg cggttatctt tcttcatctt tttataaatg ctgtaaaaat gcttgggccg
     3001 gccgttgatt tcgcagtgga tgtgggcttc gtcgagggcc ttcttgagga cgtcgatggc
     3061 ttcgttgacg atttcttcgc ggacgtggcg cttctgcttc atctggtcga cgaggtcgta
     3121 gtacttgtcc ggttcgaggt agcggaagga caaatcttcc agttcccact tgatattgaa
     3181 aatccctaag cggtgtgcca ggggggcaaa gatttccagc gtttcctggg caatacgctt
     3241 ctgcttgtcg ctgcgcatgt agcgcagggt acgcatgttg tgcaggcggt cggcgagctt
     3301 gatgacgacg acgcgcacgt ccttggccat ggccaggaac atcttgcgca tgctgttgag
     3361 ctgctggtct tctttggtac ggtaattgag gcggctcaac ttggtgacgc cgtcgacgag
     3421 gaaggctact tccttgccga acatcttctt gatatcttcc agtgtgtacg acgtatcttc
     3481 gacgacatcg tggaggagcg acgccgtaat ggtcgtcgta tctattttca tatctgccag
     3541 tatctgggcg acggccaggg gatgcaggat atacggttcc ccgctgacgc ggcgctggtc
     3601 cttgtgggcc tcttcggcca ggttataggc tttggtcacg tcatcgcatt tggcctgggg
     3661 cagatacgta tgtatcgtgt cgatgaggtg tttgaattcg acatatttat ctttaaattc
     3721 cataatatga aacctcctca ctccttgcag tacaattcat acgtcggcga cgcatgaaga
     3781 tccattttcc ccgcaaggat aggaaaataa taagctgggc cgtcgtcatc atggcgtatt
     3841 ttgaggacgc cgatttcccg gaggacgacg atggcagcgt aaatggtgtg gacatcataa
     3901 cagtccccct ggtccgcagc cagacggcgg cggacctgcc agaccggcat gccccagtcg
     3961 gggatgcatt tcttgagggc catgtaaata tcgaccatga ccgcccggtt gaggacgatg
     4021 tggcggcctg gcaggtggac gtcctggatg accagctgcg gcgacgacat gccattgaag
     4081 tcgttccgtt cgagctggaa ggccacgtcg atgacgtcgc cttcgacgat ggcgtcgcac
     4141 aggcccgcct gggaccaggc gacaccggaa aggcgtgtcc tatcggcgcc gcgggcgacg
     4201 aggcggacgt gctgcttttc ccggccgatg ggacggattt cctctaccgt agccccgctc
     4261 agggaaaaga cgggccggct gttgcccatg ccatagggct ccaggcggga cagctcagca
     4321 atgaggtcca aggtcacatc ggccggttcc aattgcttgt cgatgtggac caaggggacg
     4381 tagtcggcgg ccgtcatatg tgctgccgca tagtccagca ggcgccggcg cagggcttcg
     4441 atgttctcgg ccctgacgga aaagcccgcc gccatggtgt ggccgccgaa ctggatgagc
     4501 aaatcctggg cgtattgcag ggcttcgtac atgttgaaac cgctgatact gcggcacgat
     4561 cccttgccga tgccgtcgcg gacggtgatg accagggacg gccggtaata cgtctctacc
     4621 aggcgcgatg cggcgatgcc gatgacgccg acgtgccagc cctcatgata ggcgatgagg
     4681 acgccgtcgc ggcccctcgc ctgcccttcg atctgggtga tggcttcctg ggcaatggtg
     4741 tgctcaatgt cctggcgctc cgtgttgagg cggctcagtt cggctgcatc ggccgccgcc
     4801 ttggccgcat cggtttccag gagcagttcg acacccttcg tcgcatggct gatgcgcccg
     4861 gccgcattga ggcgcggcgc cgccgtaaag gcgatacggc cggccgtgat atccttcccg
     4921 ctgagattgg acgcatcgag gagggccttg atgcccgtcc gcttcccttc tttcatggcc
     4981 gccaagccat ggcggacgag gatacggttc tcgccagtca gggggaccag gtcggcaatg
     5041 gtccccaagg ccgccaggtc cgtatagccc ggcaggtctt catggcagtg atgctgccac
     5101 agggcccggc agaggacgta ggccacgccg gcgccggcca gctccttaaa gggatagggg
     5161 cagccggcct gcttgggatt gaggacggcc agggctggcg ggatctgttc cggcggttcg
     5221 tgatgatccg taatgatcat atccaggacc ggcgagaact gctggaccag gtcgtgtgac
     5281 gaaatgccgc agtcgacggt gatgagcagg tcggcctgcc ggctcagctg ctccagggcc
     5341 ccttcgttga ggccatagcc ttcgctctcc cgttcgggaa tgtagaactg cggcgatgcg
     5401 ccgaggtcgc gaaggacgga ataaacaaga gacgtcgacg taatgccgtc gacgtcatag
     5461 tcaccgtaga tgacgatgcg ctgcttttca gttatcgcct gctccagcct gatgacggca
     5521 ttctccatgc ctttcatcag gaaaggatcg gccatatcgg ccaaagtatt gtgaagaaaa
     5581 tggtcccctg cccggacatc ggcgatgccg cgattggcca gtacctgtgc caagaccggg
     5641 gatatatgta attgtgcgga caaggcctcc atgagcccct cgtcgggact gccgaactgc
     5701 catcgtttct ccatagctat cacgacacat tctcaagaat aatcactatc aataaatcaa
     5761 ctattatttt atcactttct attctcaaaa taaaggctaa atctatagct atttgccatt
     5821 atttgttaat tttgagaaag tttaaagttt gtttaaatat ggaaggctta aaaaaagcga
     5881 tgtcatatga agacagcgat ttttctgtag gggtcgcccc gtgggcgacc cgtttcggta
     5941 tctgggtgaa ttctctacgg agccggacgg gaaaatccat acatatccag ttgcgggctt
     6001 gccacgtgca agcccctact gcaaactaca aaatcacgat tcggctacag ccggcgcgga
     6061 gacggcgttg tggtcggcag cagggttgat atcttctttg gagatagtca ggcggtgctg
     6121 ggcagcctgc aggatgaggt cggctgtctt gacgatgccg tagtcactgg atttgaggct
     6181 caagtcgtag ttggcgccgt agccccaata ggaatcggtg cagtacatgc agtggtcgcg
     6241 ccgttcgtga ttgacgtggt tgcggatctt ccgcgccttg tcggcatcga cgtgttcgcg
     6301 gcgcctgaag cgctggctgg cccagtgcgg gtcggacgaa aggaagacat tgatggtctt
     6361 gggccgtttg cgcaggatgt agttggccag gcggccgacg atgacgcacg acttcccttc
     6421 tgtcagagcc tggatgactt cggattcatc gcggaacatt tcctgctgct tcgattcata
     6481 gcccgacgcg tagtggatat aatcgtcata gaccttggtc agcaaggggc cgagcatacg
     6541 cttgcggtct ttacaccagg cggcgaaggt ctgcggatgt tctttccggg tcaggttgag
     6601 gatttccgta tcgtaataag ggatgccgag cttacgggcg acgagcttgc cgatgttgcg
     6661 gccgccgctg ccgtattcac gggaaatggt aatgaccaag ggactttctg tatcttcctt
     6721 gatccaggcc ggatgatttt cgcggccttc gatttcaccg atagcatggt gacggaagag
     6781 caggtgttca atcggccctg ccatcgtgca gcccaggagg atcagggcca ggccgacgac
     6841 ggcattgacc gtaatcggtt ccgacaagat gatggccgac aagatcaagg cgatgatcgg
     6901 cttgatgaag aaggcaaagg atgcattgga cggacctgtc agttcgatgg ccttcatgaa
     6961 gcacagatag ccgaagcccg tgacgactaa ggaacagtaa ataaggggcc atacggtctg
     7021 ggaatcaata c
//
LOCUS       DAWCYS010000065         6898 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_674_length_6898_cov_4.743095, whole genome shotgun sequence.
ACCESSION   DAWCYS010000065 DAWCYS010000000
VERSION     DAWCYS010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_674_length_6898_cov_4.743095"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1939 a         1669 c         1935 g         1355 t
ORIGIN      
        1 ggattcgtga aggcataacc ttctgcttgg ggctgacgat ggcctgcggc attggtatct
       61 atatcggcca tacggccggc gaagaagcga agcagctgga agaacagcgc atccattacg
      121 tccaagaggg ggaaaccctt tgggacattg cagctggcat tgccagcgat agtgacgata
      181 ttcggcaggt cgtatatgaa ctacagacag tcaacaatat atctggtaca gatggcctgc
      241 atcccggcca gcggctgatc atcaagtttt aatgaggcag tcgatgacat gcccgctatg
      301 cggcaaaacg gcgaacagct ggatttattg caaggccagg gcacaggata tttgccagga
      361 ccattgtgaa gcttgccagt attttgcagg gactatgcta tggtcctgct ggtacggcat
      421 aaaacaagaa gcgccaaaga aaaagacgaa ggcgaaggaa caggaactac catatacacg
      481 cggcggccgg gccgtggaaa agttccggca aaacatgagg gcaatagaaa agcgcctcac
      541 tcataaagaa tgagacgctt tctaccttgt gttgcaaaag agatcgtcgg tacacaatca
      601 gtataacata gagcaggttt aaaggatagg gcacggccct attttacact tgcttaaagg
      661 tattaaatat acaaacaacc ggcggacagg aggttcggca tgtatgtcat ggaaacggtg
      721 acagctggtc ccgtcatcga ggtgagaaaa tatcataccg cccgctacca ccatccatca
      781 atgccgcgca gccggaattg caataagacc ggcgccgacc agtggaaggt aaatgagcgc
      841 aactccctcc ggaacttgcg gctcctcatc ctggagaact ttcaagagga tgacatacgc
      901 ctggacctga cctatgccgg ggaagctccg accgaagctg aagcaaaaaa gcggctggac
      961 aacttcatcc tatgtctacg gcgccactac cgggcggctg ggcatgagct gaagtggatt
     1021 ggcacaagtg aaggcaagga ccatcgccct catcatcatc tgctgataaa caacatcggc
     1081 tggggacggc gggagtatca agccctgtgg aaatggggca agattccata caacgccttt
     1141 cggttctacg atggccagcc agctgatgcg gaacgagtgg ccaaatatct cgtcaaggaa
     1201 acgcgcgaaa cgtactgcca aaaagagcgt tgccagcggt cccgctatcg gtgcagccgt
     1261 aaccttcgca aaccaaaggt tgaaaaggaa atcatacagt ccaagacgtg gcgggaaccg
     1321 aagcccaaga agggatatta catcgaaaag cccgtgcaat acgggtatac ggcctatggg
     1381 ttcccgtaca tgttctatcg catgataaga gaggaggaaa gcgttggtgt acctgatccg
     1441 aaaatcaccg ggcccgttcg ctgccgtggc agagggacgg gaaaatacgc tgctgctcgg
     1501 aaaaaacgaa tgtgtccgga ttgacgagga aatccacttc gtcgaatttg agcacgggcg
     1561 gcggaccggc aaagaatgtt atgggcgcgt cgagtatatc tacgataagc gcctggtcaa
     1621 attccgggta tacaagcacc ggacgaatag aaagaaggcc cgacaatgga actcaaaaaa
     1681 aagaagcgaa tccacctgaa cggcaggaag gccagggaat tttacgataa gatttacgac
     1741 cgcgacggcg gccattgcat ctggtgcggt gtgccgattg aatacggcgt gaagtaccac
     1801 catgagccgt gcggcattta ccgaagcgat gaagaaacga aagtcgtcat gctctgtcca
     1861 cattgccatt ttatccgcca ccaccaggca ccggcagtcg caagggagat ttgcgtagat
     1921 tatctgcatc gcctgtacgg tgaaaagggg gcgctcaaag aatgattgcg atttgtgagc
     1981 tatgtggcaa accatttgaa accagccaaa agcagaactt gtgcggacct tgttcggtgg
     2041 cagcggaaca gcggatttct catccgaccc atggcgtgtg ctgtatttgc ggaagcccta
     2101 tgccaaatgc cagacgtgga caaaagtatt gcagtcataa ctgcaagcgc ctgagccgca
     2161 gtattatcaa cctgcgctgg catgatgccc acccagacta taagcaggcc ccgagagtta
     2221 agccgcagaa gacgcgaagc ttcatggacg atgtagaaga cttggggcga aaaatgggta
     2281 tcggcggcta tggccagatg atggccatga ttaagacgcg ctgtcagaaa tccggcctga
     2341 gcgcacggag cgaattctgc cacttgaaat ttgtctatga aaaggagcat ggccatgatt
     2401 aaaatagccg tgtacagtct caaaggcggc gtcgggaaaa caataacggc cgccaacttg
     2461 ggacatttgt acgccactca tcgaacgaag aaactcaaag gaacgaagcg gaatagtttg
     2521 cgccgcgttc tgttagtcga ccgggacccg cagggaaact tgtcgcaata tttttgccgc
     2581 tatgatgcgg atgggccggt acagatgaag cctgtcggta cagaatgggc atggctggat
     2641 attatccctg gcaatctgtc cttatcggct atggaagaca cggccctgga cgtagatgga
     2701 tttgccgaag actacgatct ttgtctcatt gattgcccgc cagctttggg aaaactgacg
     2761 gccgatgcct tgcggtgtgc agactatctg attatcccta tccgcctgga tgccttcagc
     2821 acgcatggac tggaaaatct catgcagcag ttaagctact tgcaggaagc gggctttgat
     2881 gcgaaagtgc tgggcgtcct ggtcacgcac gatgagccgg catggtacag tgatgacgta
     2941 aaggctttgc ttggcgaacg gctgccgcta tttacgacgg ccatccgccg cagcacctgg
     3001 gtggctgaat cgactatcga gcataagccc ctggcagaac tgaccgggcc ggaccatcat
     3061 ttaaagccaa tgtgcatcaa gccggcctgg cagtatcggc gtgtcatgaa tgaaatcgtg
     3121 gacaggttgt ccaaattgga caggaggtag cacatgaatt tagcagaaag cctgggcttc
     3181 gtaccgcaaa agacggcacg ggccgtgcgg gctatcaatg taggcaagct catcccgaac
     3241 ccggcgaact tttaccacat cggcagtctg gatgagctga aagccagcat tttagaagac
     3301 ggcggcgtcc gccaaaacct catcgtcgaa ccgaagggcg atggaacgta catcatcatc
     3361 agcgggcacc ggcgctgtca ggccgtcaaa gaactgctgg ccgacggggc cgacgtggaa
     3421 gcggacctgc cttgtgaaat cgagccggac catgcgaagg cggaacgatt actaattagg
     3481 gccaatagtg cggcccgtat cctgacgcca tgggaagaag tcctgcaagc gcagcgggcc
     3541 gatgaaatca tcacccgcca gcggcaggaa ggcgtcatct ccgaaacgaa gcggcaggcc
     3601 atgcagacct tactgcataa aagcagcgga accattggac gcctctgcgc catttacaat
     3661 aacctcatcg tcgacttaca gcagaaaatg aaagacggaa aactcggtgt atccgtggcc
     3721 tatgaaatct gccagttacg gccagaagcc caaaagggcc tgatcgatgg catgaccaaa
     3781 ttcgacatag aagaaatcag cctggccgat gtccgccact ataaagacat gaacggcatt
     3841 agtaataagc cggccgatga tccacggcag acaactatca tggacttcat cgacgacaag
     3901 ccagccgaag agccgccaga agatacacgg acaacagtgg aagccactca agagcaaatg
     3961 gaacctgcgg tggtggaaac ggcacctgca gaagacctgg aagaaaaaaa ggatacctgg
     4021 acggcccaga ttgacttgaa aaatttccag acagtagcag acagcgtctg gcagataacg
     4081 tgcagtcttt ggagactgca tgataaatac gtcgacggga atatcacaac ggaaaattat
     4141 atcgccgaat cacaatatga agcacggacg ctgtatcagg aactggatca cctgcgggtt
     4201 cttcacgaaa aaacgaagaa atataaaggg gagggagaaa atcaatgaaa tgggtagcaa
     4261 cgagggaaga aatgcctgaa gtaggacgcc gcgtgttatg cgcgatgtac gccgacaccg
     4321 aatacggatt tccagtatgt ggagtttttg atggggaata ttggattgtc gacgatgtac
     4381 agcagcttat cgaccagaag agggtacatt actgggcacc gatagcacgg ataccgaagg
     4441 aggaccgata gtgtggacct gaaacggatt gtttggacgg caatgattgc ctgggcgatt
     4501 atcttttggt tcggaattac gtatttactg gtgaggtggt aaggtggaca ttgtaaaagc
     4561 cggtatcgta gatgcccgaa aattcttact gataattcgg gaacagcatt acgagctgga
     4621 agagctgaag tatgagcgct acctggagga aaacgggcta tgcctgaaag tctcgaaccc
     4681 ggcgcggccc tgtgtcagcg ctggcggtcc taacgactta tcgcgtatcc ccgttcacat
     4741 cgaacaattt gtgaaaaaga ttgcccgcga agaagcgaca ctctatcaga tccgggaaat
     4801 gggaaaagac tttatatccc tgcttccaga tgccaggtcg cgggctattt tgaaatacta
     4861 ctacgtcgac ttcctgacat gggaacaagt cgccatgcgg attcacttat cgccgtcccg
     4921 gacatataac agtcaccgcc tggcactgga acagctgaac agtctgatac gggccgcatg
     4981 gatgcggaaa tttatcgaga ttttaaaaga caggagtaaa taggagtcga acctgtggta
     5041 aaatgatagt gtcagaaaaa aacaaaatac gggcgctcgc ctaaccgcgg agcgcctttt
     5101 ttgtacccag aaaggggggg acggccatga ttcactgcga taacatccgc tgcaagttca
     5161 atcaaatgga aatctgtacg aacctgcgcc tggaaatcgt ccatgagcgc tgcgtgtgtt
     5221 ttgagctgaa atatcatcgg gcgaaacgcc atgtgtcgga tctgaaccat gagccggttc
     5281 cctatcggtc cagaaagagg ggctttaaat gacagccaat aaaatccggg gaaccccgat
     5341 tcgccgggaa aagatatttt taaataaaaa taatacgcgc ccaaatagcg agcgcaaaat
     5401 aaaaactaag aagcgccgcg ctgtgacctg ggctaaattc aacacggcga aaaacatgat
     5461 gattgttcag tctatgtgcc gcaagggatg gactaatgat gaaatcgccg actacatcgg
     5521 tatcagcctg tctacgtttt acaaatggca ggccgaacat gtagagtttt cggaggccct
     5581 caaagagccg aaagaatact gcatcgcccg cgtagaaaat gcactcctga cgcgggcctt
     5641 gggaatcgaa aagaaaatcc acgagtccga aactgtcacc gtcgaaaaag acggcaagaa
     5701 ggttacgacg acaacggaga aaaacagcct ctgctactat ccgccggaca cgcgggccgg
     5761 tatcttctat cttacgaacc gggccgggag tgactggaag cagaagcagc agacggaagt
     5821 aacagggaac ctgagtatcg atgcggctgt aaatacagag ggccggctca aagccgctat
     5881 ggaaaggaag aaaaaggaat gaccatagat gaagcttatc aagtcataga ctgtctgggg
     5941 caagtaagtg acgatccgga agcctttgtc tggtttgctt ttgactggga ccatgacccg
     6001 gatttgcagg gtcaggcgcc acaggactgg cagctggaac agctaaggat gatagggaaa
     6061 gggctggcta cgccgaatga agtcattcat caagccgtgg cctctggtca tggtatcggc
     6121 aaatcggcgc tggtcgcctg gatcatcttg tgggctatct caacgtatcc caatacgcgc
     6181 ggcgtcgtca cggcaaatac agaagcacag ctgcggacga agacctggcc ggaactcgcg
     6241 aagtggtatc ggcgtttcat cggaaaagag ctgttccatc tgacggcaac gtcgctcttt
     6301 tccatccagg aagggcacga ccggacctgg cgtattgatg ctatcccttg gagtaaagac
     6361 aatccggaag ctttcgccgg gttgcataat cagggcagcc gaatcctatt ggtattcgat
     6421 gaagcctcgg ccattgacga tgctatctgg gaagtcgccg aaggggcgct gaccgatacg
     6481 gatacccaga ttatctggtg tgccttcggc aacccgacgc gcaacaccgg gcgctttcat
     6541 gattgcttta cgaaataccg gaaatactgg agcacaaaga agatagacag ccgatctgta
     6601 cctatatcga acaaagccca aatacaacaa tgggaagacc aatacggtga agactcggac
     6661 ttcttccggg tccgtgtacg cggcgaattt ccggctacat cggaaaatca gttcatctcg
     6721 gcacaactcg tcgaagacgc gcaaaaacgc gccatacggc ccgctcagta caattttgcg
     6781 ccggttattc tcggcgtgga catggcatgg aggggcggcg atgcgagcgt catgtttctg
     6841 cggcagggac tgtacagccg caagcttgcg gcggtcgcga aaaacgccag cgacggcg
//
LOCUS       DAWCYS010000066         6889 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_679_length_6889_cov_5.964004, whole genome shotgun sequence.
ACCESSION   DAWCYS010000066 DAWCYS010000000
VERSION     DAWCYS010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_6889_cov_5.964004"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1675 a         2067 c         1872 g         1275 t
ORIGIN      
        1 cgttccagca accatcttgc gccttccgtt ggtcggcacc cgatgggctg gaaagacttc
       61 aggacggccc ctactagcca ctaccccccc atttgtggta aagtagaacc atatcatcat
      121 tgcagagggg agctatgaga atggaaaaaa agaagatata tgtactggct tgtggcggga
      181 cgattgccgg caaggccgcg tcggccgatg acctgacggg gtatgctgcc ggtcagacgt
      241 cgatagatga actgctggaa gccgtgcccc agctggctga ttatgccgac gtgcagggcg
      301 aacagttctg caacatcgac agctcggata tgaccgaagc tttatggctg tccctggcca
      361 atcgggtcca ggaagtagcc gaccgggaag acgtggacgg tatcgtcatc actcacggca
      421 cggatacgat ggaggaaacg gcgtatttcc tcgacctcac ggtccacacc atcaagcccg
      481 tcgtccttac gggttccatg cggccggcga cggccatcag tgccgacggt ccgctgaacc
      541 tcctggaagc ggtccaggcc gcgtcctggc cggacctggg aaaatacggt gtcgtcatcg
      601 tcatgaacag caccgtccac agcgcccgct tcgtcgaaaa gaccgatacg acccatgccg
      661 atacttttaa aggccgccag atgggctgcc tgggctatat gcaggacggc cggccccgcc
      721 tgtaccagca gcccctgcgc aagcatacgt ggtcgtcgga cctggccgct cctaaagaac
      781 tgccagccgt cgccatcgtc tatgcctatg tcggcctcac cgctgacgac gtacgccgcc
      841 tggcccaggg gaaagcggga ctggtcatca cgggtctcgg tcatggcaag atgcccgaac
      901 tggtctggca ggccttgcag cccttgatgg aacaggggct ggtcgtcgtc cgggctgccc
      961 gcgccctggg cggcatggtc acccaggtcc cggcttatga cgggacggtc acagccgatt
     1021 ccctgacgcc gcagaaggcc aagatcctct tgcagctggc cctggcccac acgacggacc
     1081 gggaaaaaat acaggccatc ttcgacgcct attgaggcct gcaaatcacg gctctatgcc
     1141 ttccctaata gggcggggtg tgataaaata aataagatat gaaacatagg tatgcaatcc
     1201 atgtatcctt tacgacactg acatagatag gagcgaatga atcatgaaca agtacaaaaa
     1261 attgatggtc cttacggccc tgacagccgc cctcggcacg tcggcttttg ccgcatcgac
     1321 ggggattacg gatatcagca attattgggg caaagacgct atccagtatt tttataacca
     1381 gcactacatc agcggcacga acggccagtt ccgtccgaac gaagacatta cccgtgaagg
     1441 cgcagctgcc atcatcaaca acatgatcgg tgaagattcc aaagtcaaga cgaccaactt
     1501 cagcgacgtc aaaggccgct ggtccgaacg ggccatcgcc tcgctggtcg acaaacagat
     1561 catgagcggt tacagcaacg gcacgttcaa accggaacag aagatcaccc gcgaagaatt
     1621 tgctgtcatc gcttacaact acatgaccta taagggcatg agcaccctcg aaggggccgc
     1681 tccctatgcc gacgaagcca agatttccag ctgggcccgt caggccgtcg acgccctggc
     1741 cgctgccggt tacatgaagg gcggcaacta caacatgttc aacccgaaac agtatgtcac
     1801 ccgcggtgaa gccgtcaacg tcctctaccg catcctgacc ggcgtcaagg aaacgacgca
     1861 gagccaggac ggcctggaat ccaaggcctt caaggatatc aaagacgtct acggctcaat
     1921 caaagccttt gcatctgacg gcatcatgta ctggcagggc gacaagctcc acatcggcgt
     1981 caaagacccg aagaacaaac agaaactggc cgatgccatt gctgccgaca aggatatccc
     2041 ggccgaatcg gtctatgtcc agaaatcgac gtacagctat gacgactata agaacctcat
     2101 ggcccaggcc gaaaagattt acaaggctac ggaagcgacg aatgctaccg tttcgacgga
     2161 accggattac ctcaatgaaa aagtcgtcct cacagtcagc tccatttcca aagaaacaca
     2221 gaacaacctc aataaagccc tgggcagtgc ccttcgcatc gtcattcagt aatcgcagca
     2281 gaggaggatt tccatggtac ggaataaaat cgctgccatc ctggcagctg cggccatctt
     2341 gtccgtcagt gccgtttcgg ccgatgcccc ttatacagac ctgggcagcc tgaccgaccg
     2401 cgcggctgtc gattacctct atgatacgca gtgcctgacc ttcatcacag gggatacctt
     2461 tgagccaaac cgggtcctga cccgcggcga cctggcccag ctgctctata attcggcagc
     2521 caatatcccc ctggcaccgg tggaagcggc cggtgcccac gatatgccgt cgggcacggc
     2581 tggtgatgcc atgaacagcg tcgccgccca gggtatcctg gcaggctatc aggatgggaa
     2641 tttccatccc gaagaaccgg tgagccgcga agaatttgcc gacgtcatct accgctactt
     2701 gcagtacaat cagctggccg acccggacca ggccgtcaag ccctatgccg atgccgcgtc
     2761 gattgcgccg gcttacgcca gggccatcga cgtcctgcac agcaagaaca tcatggtgcc
     2821 ggcagataac tatttccatc ccaaagaagg catgacccgg gccgatgccg cccaggtatt
     2881 ttaccgcctc atgcactccg acggggacta tacgtctcat gtccaggtcg aatcgcagct
     2941 catcaaggcc atcaatgccg aatacggctc agtgccgatc tacttccgca gcggtacgat
     3001 gtactgggat ggcgataccc tggtcctggg catcaaaggc gctcccagca aatacttgca
     3061 gcagcgcatc aacacggacg tagccaagcc gcaggccgtc aagatacgtc gcgtatcctt
     3121 gagccacagc gattacagcc agctcatgaa taaggccatc cattgcgtcg tcgacaacga
     3181 aggcgtccag aactacgtcg gcgccctgcc ggactacgtc aatgaacaag tcgtcctcac
     3241 tgtccgccat cccgtcagca aggcgacgct ggctgaactg gccaaacgcg tcggcgccgg
     3301 ccgggtccgt ctggaaacgg ctcctatcgc cgggaaagct cccatcgtcc aggtagccgg
     3361 acagacagac acagcggaag aagcccctgt gacgacagaa aacacgaaaa aagaaacgaa
     3421 acaactctat tccaaactct tagacgacgc cacatcgagc gccatcacct ccgtccagaa
     3481 cgacgtgatg aagtgatttg taggggacgc ccaaagggcg tcccgcaggt cgcagttcgc
     3541 aaaagggact gtcgcatgaa ggtttattcc atcatgcgcg gtccctttct ctttatagtt
     3601 tgtagtacgg agtttgtagt ttgtagaaaa tggctttaaa tttaaaagca ttcactaaaa
     3661 actacaaact gcaaacttaa aactcaaatc acactccgaa gagttctccc aattcttccc
     3721 gcgtgtcgat gaggtatgtc ggctggagag attccaggaa gtcccggtcg cggaaacccc
     3781 aggtgaccag ggctgcgtcg aggccggcgt tatcggccgt ttctttgtcg acttccgagt
     3841 cgccgacgta gatggcgtcg tctttggcac agcccaggcg cttcagggct tcccagacca
     3901 tgtctggtgc cggttttttg cggtacatcg gcgtttcacc caaggagaaa tcgacatagt
     3961 ctttgaagta ttcatcgtgc agttccgata cggcaccctg gcctttgttg gagacgatgg
     4021 ccatgtggta gccggccgcc ttgagccggc ccaggacctg agggatgccg gcgtagggga
     4081 aggtcgtcgt ctggcagtgg gcgctgtaat aggcgatgaa ctcttccgtc ctcgtccgcc
     4141 gcaggggttc cggcgcgtcc ggtgcggcct tctcgatgaa gccggcgtat ccgaagccca
     4201 ggcaggggcg gatggcatcc tgcgtcaggg cgggcaggcc gtgacgggca aaaacatagt
     4261 tcaggctttt cgtcaattcg tccagcgtat ccaggacggt gccgtccatg tcgaagccga
     4321 tggctttata tttttccatg aaaaagactc ctttgaagga caaaaaatca gtttactctt
     4381 ttattatacg aaagtgcgaa ttaatcggca atagggagta gctcatgccg tcatcttatg
     4441 ctacaatggg aaaagaagtt tgataaaata gaggagagga tgacatgaca aactttttgc
     4501 cgtacgctat catagcgggc atagcaatcg tcttttttgt aatttgttat attatgtatt
     4561 ccggcggagg gcactctgag agtagtcccc aaacggagaa gaaagggaac acgaagacga
     4621 agacgcgcta cgacggcgag ccgcagccgc tgcgcagggt ccagcaccgc agcccggcaa
     4681 cagttaagca tgccagacaa ttaccgaagg aagcggacga cgaagaaaca gcccccttgc
     4741 cgcaggtccc gaaaatgaca ctgggcgaac ggcgggagca tccggaagaa acggcctttg
     4801 tcacgaccaa aggcgaagcc gtccgccgca atagccgaat cgtctataaa gaagctccca
     4861 aagcccagga acctccggtc cacaagggcc ccaatgcgga tgacctcgat gcgacgcgcg
     4921 tcctgtcccg cgatgaaatc ctggaagcca tggaaaaggt agacaaagaa gaagccgctg
     4981 cctataaggc ccgcgaggaa gaagccaaac tagcccgtca gcgccaggcc gaacaggaaa
     5041 aagaagccca ggaacagcag ctggcccgca accgggaacg gcgcgaacag gcccgccgcg
     5101 aagccgctgc ggccaaggaa gccaatgccc gccgggaaga acgggcacgc tgggccgaag
     5161 aagtagccca gaccgaagaa gacaagccgc ccctggctga tatggccgcc atcgtctcgg
     5221 ctgacctggc cgctaaagat ggcggcgccg ccccggccgc caggaaacag gctgccccgg
     5281 cagctgaacc ggcagcatct gaaacagaag cgccgaaaga cacgcaccgc aagcacattc
     5341 tccgcgacgt catggccgaa acggccaaag ccgccggcac agccaaagcc gtcgccgacg
     5401 caccggctgc cgccaagaaa cgggccgtat ccgaggcgac acaggtcctg gaaggacctg
     5461 tcgtcagcga aggcatgaaa gccgtcgctt cccagctgga acagacccag cgcatggaac
     5521 cgatccacat cgactcggcc atgacggcca agaagaaagc cgtcccggaa gaaacaatgg
     5581 tcatgccgcc catccgggcc cacaagtccc aggaaaaagc gacacctgtc gtcaagccgg
     5641 caccggtcgt caaaccgtcc cagacgtcgt gggattccaa tacgtccggg accgatacgc
     5701 gcaacccgtc actgggcaat gcgccgcact atgcgtccgt atgggaatcg gaaagcactg
     5761 tcgattcgcc tgtcgtccgc cagtgcgtct ctcacttcct gcgccagtac ggcgtcgttt
     5821 caccggaact gcagcagcag acggaataca tcacgtcggc ggcctttgac cagatcggct
     5881 gccagaccga tacggaacgg cagcaggccc tgtcaccgct catcgtccag gaagctttgc
     5941 agaacgtcca gaaagcctat gccgctcatc ccgatgatta cgtcggcacc attgccctgc
     6001 aggcctttta cgatatcgtc cactgctcgc ccatttcgac gcgccatctg gtcgccatcg
     6061 atgccttgaa ggtcatgccc tatttgaccc agggccatta ccagatcctg gcgattctct
     6121 tattgttcct gtattcgcgc aactcccata acgtcgacaa ggattcgttc tgccgctata
     6181 tcgacaagta cgtctatccc ttcctggatg gattcccgac ggaacggcct tactaccagc
     6241 agctcgatta cttgcactgc acggcttttg aagccaagga aacgcatttt gccgaaatcc
     6301 tgtccgattc gtatccgctc ttgttccgct accgcggttt cacagaagaa gaattgcgca
     6361 aagccctcaa aggcaagcgc atccccgatg aattcatcgt cccgtccttc aattcgccac
     6421 tggtcaaact ggccatggtc gatgaaagca tggccaaacg cttcttccgc atgaccggca
     6481 tcaacgaccg caacatgcag gaccacatcc tgcgcctggc caagaaacgc ccggccaact
     6541 tcagcggcga agaagccctg gacatcatgg aagatatttc gccggtcctg gccgacttgg
     6601 gcgatatctg ggacagctcg ctgctgcgcg tatcgacgct gtcgctcctg ggcctgtact
     6661 tggctcaggg cttcgtcaag gaaatcattg gcgaagaatt cgatttgtcc cgctggtttg
     6721 aataaggagc atcgttgtga aaaaaatccg taaagccgtc atcccggcgg ccgggttcgg
     6781 cacccgtttc ctgccggcga cgaaggcgac accgaaagaa atgctgccta tcgtcgataa
     6841 accgaccatc cagtatatcg tcgaagaagc cctgcagagc ggcatcgaa
//
LOCUS       DAWCYS010000067         6850 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_684_length_6850_cov_5.488742, whole genome shotgun sequence.
ACCESSION   DAWCYS010000067 DAWCYS010000000
VERSION     DAWCYS010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_684_length_6850_cov_5.488742"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1714 a         1812 c         1657 g         1667 t
ORIGIN      
        1 cgtcggtatt gatctgttat cccgtaccgt gcggcgtcat gtatttcttc atgcgcagta
       61 tgggcgaaag gctgtatcac gaacctgtta ctggctagtt tgccacttat gggcataagt
      121 atatcagtcg tttcgtcggc tacctcactg cctgctccta ttggttcttg tgggtcgtcg
      181 tcggtctgtc ggaaatcaca gccgtcggca tctatgtcca ctactggttc ccattgctgc
      241 cgcaatggat atcggccctg gccggcatgg ccatcgtcgc tgcggcgaat atggcggccg
      301 ttaaatatta cggcgaattt gaattctggt gcgcccggat caacgtcgtc accatcagag
      361 tcatgctcat cgtcggtgcg gcggtcatcc tctttggctt cggcaacggc ggcgtccccg
      421 tcggcatcag caacctctgg gtcaacggcg gcttcatgcc taacggctgg ggcggtatgc
      481 tggcggccat gtgcgtcgtc gccgcgtcct tccagggcgt cgaactcatc ggcatcacgg
      541 ccggcgaagc ccaggacccg aaacatactt tgaagaaggc cgtcaacaat atcgtctggc
      601 gtatcctcat cttctacatc ggctcgattt tcagcatcct ctgtatctat ccctggaatg
      661 aagtcagcta tatcggcagt cccttcgtca tgacctttgc caaagtcggc atctcggcag
      721 cggcaggcat catcaacttc gtcgtcctca cggcggccct gtccggctgt aacagcggca
      781 tgtacagctc aggccgtatg ctctatacgc tggcccagaa cggccaggcg ccgaagattt
      841 tcgccaaatt gtcaccgacc ggcgtgccgc gcaacagtat tgccgtcacc atcgttgtcc
      901 tggctatcgg cgtcatcctc aactatctca ttccggactc caaattgttc ctctatttat
      961 acagtgcctc cgtttttccg ggcatggtcg cctggttcgt cctggcctat gcccagaaaa
     1021 atttccgcaa agcctgggga ccggaagtca tgccgaaaca tcctttcaaa tcgccgctct
     1081 atccttattc caattacttc tgcatcattt tcctgattct cgtcaccatc ggcatgattt
     1141 acaatcctga tacgcgcatg tccatcttct cgtcgtgggt ctacatcgcc gccgtcgtca
     1201 tcgcctggtt cgcctgtggc ctgaacaaga aacagtacga taaataaggg cacctcatcg
     1261 aaggggctgg aggagatgcg gcgacggtcg ccaaaactga atcggaatcg tgattatctg
     1321 tcgcaggtct atggctttcc ctgggcctgc gatgtataat acagttagat taaaattaag
     1381 gagatgcata ctcatggcag ttaatgttat cagcacgact aaagctccgg gcgcagtagg
     1441 cccgtattcc caggctatta aagccggcga tttcctcttc gcatccggtc agattgccat
     1501 caacccggaa aaaggcaaga tcgtttccgg cggtgtcgtc gaacaggctg aacagtgcat
     1561 gaagaacgtc ggcgctatcc tcgaagaagc cggcctctct tacgatgatg tcgtcaagac
     1621 gacggtttac ctgacggaca tcaacttctt cggcgccgtc aacgaagtct atggcaaata
     1681 cttccagaag aacctgccgg cccgttcgtg cgtcgaaatc agccgcctcc cgaaagacgt
     1741 cttcgtagaa gtcgaagtca tcgcctattg cgggaaataa gctggattta tagtggctcg
     1801 tgattcgtgg ctcgttattc gtagggcggc ccgctgtgcc ttaaatttaa aactatccgc
     1861 taaccactaa caactaaaag ggactgtcgc atgaaggctt ctgctatcat gcaaaatcct
     1921 ttagacttaa aattttttcg ttcgtagttc atcattcatc gtgataggtt gatatttttc
     1981 atcccgtacg acaaacgatg aacgacctac gaccaacgta aaaaagcgct gtcttcatgc
     2041 gacagcgctt tttttacctc ccaagcagga aatcgggggt ctcatgtcta atacttataa
     2101 tataaggaaa aggagtgata actatgttta aacgtacggc atcggatatt atggaaaaga
     2161 atttcgttac ggcaacgccg gaaacgtcgg ccttcgattt ggtcaacatg ttcgtcaaga
     2221 accacattac ggccattccc atcatcaatg aaaaagatga gctcgccggc atcgtcaccg
     2281 atgcggattt gctctacaag aaggtcaaac ctcatgtccc tcactatgtc aatcttttgg
     2341 gagccagcat ttattataac ggcatcagcg aatacgacaa ggggttcaag aaactcatgg
     2401 cctgcacggc gaaagatatg atgaccaaag acgtcatcat tgccgcaccg gatgcggaag
     2461 tcgaacagat cgccggcgtc atggtggccg aacatctgaa ggtcattcct attgttaaag
     2521 aaaagcacat cgtcggcatc gtcacccgcg gcaacatcct cgatgaattg taccgtgaat
     2581 acggcgacga ctaggggaaa gggggaatag acaatggctg aaaacatcaa taaaggtccg
     2641 ggaccgggcg aagacaagga agcggaacgc cctgtcgtcc gcgtcaaaga tgtcatggaa
     2701 aaggttttcg tctcggtgac accggatatt cccatccgcg acgtcttccg catcttcatc
     2761 aagcatcgcc tgctggctgt gcccgtcgtc gatgaagaag accacctcat cggcctcatt
     2821 tccagcgccg acctcatgta ccgcagttcg aagccacaca ttccgcgcct gcccttcatc
     2881 ggcaccaaaa tttataccag ccgcgtcggc aaatacgcca aggaattcaa gaacctcctg
     2941 gatcagccct gcgaaaaatt gatgaccaag aacgtcatga tcgcgacgcc caaggccgat
     3001 gtcgaacagg tcgcagccgt catgatcctg gaacacttga agggtcttcc tgtcgtggac
     3061 aacaaccgcg tcggcggcat gatcacccgg gcctgcatcc tcaaagattt gtaccgggaa
     3121 tggaagtatt agacctttgt gactagtcac tctctgcctc gtgtgctata atattataat
     3181 cgaatgattt aggcagcggc cgctgccgca gaaagtattt cacgaggagg cacttaatgg
     3241 ataccaaaga actcgtcatc gttgtcgatt ttggtggtca gtataaccag ctcattgcac
     3301 ggcgcgtccg tgaaaaccac gtatactgtg aagtataccc gtatcacaaa gcagttgcaa
     3361 agattaaaga acttcagccc cagggcatca tctttaccgg tggtcctggc agcgtctatg
     3421 aagataatgc gccgaagatc gaagaagaag tcttccatct cggcatcccc gtcctcggcc
     3481 tctgctatgg catgcagttc atggcccaga ccttaggcgg cgaagtcaaa cacgccgaca
     3541 tgcgcgaatt cggcaagacc gaaatcgatg tcaatacggc ttcgcctttg ttcaaaggcc
     3601 tgcaggaaaa agaaatcgtc tggatgagcc atcaggacta cgtcgcttcc atcccggaag
     3661 gctttgaaat cgtagcccac acggccaact gcccggtcgc tgccatgcag aacccggcta
     3721 agaagctcta tgccatgcag taccatccgg aagtgctcca tacggaacac ggcaaggaaa
     3781 tgctccataa cttcctcttt gaagtctgcg gctgcaccgg ttcgtggact atggccaact
     3841 atgccaagac ggctattgcc aatatcaaag aaaccgtcgg cgacggcaaa gtcgtcctgg
     3901 ccctttccgg cggcgtcgac agctctctcg cagcagccct catctccaaa gccgtcggca
     3961 gccagctgac ctgtatcttc gtcgaccacg gcctgctccg caagaacgaa ggcgacgaag
     4021 tcgaagcggc cttcaaggac agcggcatga acttcatccg cgtcgatgcg tcggaacgct
     4081 tcctgaagaa actcgaaggc gtcagcgacc ctgaacggaa acggaaaatc atcggcgaag
     4141 aattcatccg cgtctttgaa gatgaaggcc gtaaaatcgg ttccgtcgat ttcctggccc
     4201 aggggaccat ttatcccgac gtcattgaat ccggtgccgg cgatgcagcc gtcatcaaga
     4261 gccatcacaa cgtaggcgga cttccggctg tcgtcgattt caaaggcctc atcgaaccgc
     4321 tccgcaacct cttcaaagac gaagtccgcg aactcggtgc cgaactgggc ttgccagaat
     4381 acctcgtatg gcgccagcct ttcccgggac cgggcctggc tatccgcgtc atgggtgaaa
     4441 tcaccaaaga aaaactggcc atcctccgcg atgccgatgc catcttccgc gaagaaatca
     4501 ccaatgccgg catgcagcgc gatatcaacc agtacttcgc cgtcctgacc aataaccact
     4561 ctgtcggcgt catgggcgac ttccgtacct atgactacac cttggccctg cgcggcgtca
     4621 ccacgaccga cttcatgacg gctgactggg cgcgtatccc atacgacgtc ctcgataaaa
     4681 tttccagccg catcgtaaac gaagtagacc acatcaaccg catcgtgtac gacatcacca
     4741 gtaaaccacc cgcaacgatt gaatgggaat agttgcaaat tgcctcacaa atcgcttaac
     4801 caagccgttt gtgaggcttt ctttttttca aaagttccaa aagtgtacca gaaaaattta
     4861 aaaaaccttt tttagggcct atttttgcca aatttctcga acaaaaaatg tgcattttta
     4921 tacgtataaa acaaaagcgg ttacaaaagc cgagtagatt tgcaatgtaa aaatatacac
     4981 ggccaacggc cttgtgcgta ggagccgacc cggacagggt ggcctgctgc gatatttaac
     5041 aacacattgt tctaacatta tggagagcct ttacgaatca cgaacaccga gctgcgtgtt
     5101 tttcctcggt ttcatatgcg gcttcgccgc gagatactca aatcgtatta cgaacatgtg
     5161 ccactcgtca taaattacgg ctacgtacag gattcacgcg ggctcgccac gtgtgagccc
     5221 ctacgatgcg gcttcgccgc gacgaactta tgattgtatt acgaccatgt gccattcgtt
     5281 ataaattccg gctatgcaca aaattcacgc ggcctttttg gcccgtcagg cgccgaccat
     5341 aagaaggcgc aggacggttg ccaaaacgga cagctgtggc tgcccgcctt tatcaggacg
     5401 gcccctacag aatcgcagct aggcgataaa tggaaacaac gttcaccgga cataaaatat
     5461 acacggccaa cggccttgtg cgtaggggcc gacccggaca gggcggccca ctgtgagatt
     5521 ttaccgtagc ctgttctgat ataatggcag gtctttatga atttgaaatg gacaaagcaa
     5581 acaggcgctg tcttcgcgtg acagcgcctg ttatactaga tttttgatga ttgaattttt
     5641 caagtagacg gcaatgtctg cctgtgatat actgaatccg aggttttgcc taaaacggta
     5701 ggcggttggt cccttgcttt tcccggaggg actttgtgtg cccctccaat ccaactggaa
     5761 aggaggggag gacaatgact ttaacggtgg atctgtttat cagtatacta tctttcggta
     5821 tggccgcatt tgcaattggt tatgccctag gtcagagttc aaataaaaca caaaaatagc
     5881 cgccagagac ccccatctta agcgactatt tttgttgact tatcggggcc aaccgtctat
     5941 cggcaggacc tcttttcatg tctattataa cgtcatttat ttatcttcgt caagcggata
     6001 ctatgtgatg gatttaggca gttagccttt actgtacagt tgtggagtcg gactcatatg
     6061 cggcttagcc gcgagatact caaatcgtat tacgaacatg taccattcgt cataaattcc
     6121 ggctatgcac aaaattcacg cggccttttt ggcccgtcag gcgccgacca taagaaggcg
     6181 cctgacggtt tccaaaacgg acagctgtag ctgtccgcct ttatcaggac gccccctaca
     6241 gaatcgcagc cagacgataa atggaaacaa cgttcaccgg atacaaaata tacacggcct
     6301 ctggccttgt gcgtaggggc cgacccggac agggcggccc tctgtgagat tcaaccatag
     6361 cctgttcgat ataatggtaa gtctttacga atcacgaatc acatgatgtg tcattaattt
     6421 tgaatttata aacttccttt gcaggacttt ataagttttt tgtagaatct atatagcata
     6481 tattgagatt gagaggtaag tctttttaag gagaatgagg catatgatga aacgaattgt
     6541 aagtatgttg ctacttactg gaactttatt gataggaaat acagtgtttg ccttttatgc
     6601 agatgaaagc tctgctgtta atcttgggat ggttcaatct tatagccgtt cggcaatcaa
     6661 cgttattgat gatgacggca cggcgtatac ttttgtttat gacaataggc ctcattcgga
     6721 cttaggtttt tggaaacttg aaagcgaaga ccagtggcat caatgggaat tcggcttgca
     6781 acaacaatct cacatggaac aattggctag ctgtggtttc tatgcgttga agactcttgg
     6841 aaaattgtaa
//
LOCUS       DAWCYS010000068         6708 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_699_length_6708_cov_5.966481, whole genome shotgun sequence.
ACCESSION   DAWCYS010000068 DAWCYS010000000
VERSION     DAWCYS010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_6708_cov_5.966481"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4465..4474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1395 a         1871 c         1777 g         1655 t
ORIGIN      
        1 agtccacata ggcctgctgg ccactttttt catcgaccag ggacaggcgc agggacccgt
       61 ctttagccgt gacgaaatgg ctttccgtga cggtgaagtc cttttccttt tccccgacct
      121 gggccagggc cgcggccttg gtcagttcca tcaggcggtc gtcgaggccg gcttcaaaga
      181 tggcgatttt ttccagcaaa tcggctttcg tccgaaccag gcgcatgaca taccccgccg
      241 tgtccaccat agccttaaac tgggcctggc cgtcggccat atcgcggtcg gcgttttcct
      301 ggtccttgtc ggtcggcgcg tagaagacca tgaaatggtg gtccatgtcg tgatataaaa
      361 agccgtaatc gacgtggacg tccgtcccgc agtgcgggca gtgatagcag aaagcctcct
      421 gcgacttgac gcggtccttc atatccggat ccaatttcgt attgatactg tcccatacgg
      481 taaagatacc ggccttctgg catttcggac aagtaatgcc tacttcatga tgcatactca
      541 tggcagttcc ccttttctgt tttcccctat tataccatat atttcgtggc tagtggctag
      601 tggctcgcgg ctcgtggctc gtggctcgcg gctattggct cgcaaaggct ctcctgatag
      661 gagagctgtc agcgcagctg actgagaggt ttcccaaatc atgacagaag tcttcatgca
      721 acagcccttc aggccgcgct ggtcaggcgc aggccggcga taccggcgat gatcagtacc
      781 agggacagga cctgccaggg cgtcgtcgat tcatggaaga ggaaaatccc ggctacggcc
      841 gtaccgacga tgccgattcc cgtccagacg gcataggcaa tgccaatggg caggtggcgc
      901 agggccaggc tcaggaaaac caggcttgct gccatgctgg ccagggtgac gatactcggt
      961 acgagttgtg aaaatccctg ggtatacttg aggccaatgg cccagcccat ttcaaagaca
     1021 ccggcaatca ataaatacat ccaagacata cgaatcgctc cttttcattt ccgccgaaaa
     1081 aaagtacgcc catactattc cttcaaaggc ctaacggaat agtacaagcg tacccggcgg
     1141 taatctgatt taattttaca gcagcctcgc tgaggttacg agcttcactt tatcacaaaa
     1201 caaaatggct gtcaacaaaa aagcgcttac caggaaaagg tcggatggtc ccggtcgatg
     1261 atgcccgtat gggtcataca gccaaaggcc tggtagacgg cgtcggacaa cttctggaca
     1321 gcttcggcga cggctttttc attgactccg taaaacccgt cgatagggaa caagatggcc
     1381 tgcgtatctt cgctgatttt cccatcatcg ctctggcgcc aggtccagcg ccgggtcttt
     1441 acggtatggc cgctgacata gaccagttcc cgttcgtccg gcttttccag ctcgccaccg
     1501 cccatcggta aaaaagtatc accttcggca gccaggcgga cttccatgcg gccatcgcag
     1561 aaattcccaa catcgtgggc gccgatgggc aggcgttctt ccaaggaaat ggcgttgccc
     1621 aggtcgacgg ccggattgat atggggcagg gccttcttct tctgcacccg tttggccagc
     1681 gcttcgatag agcaaagata tttattgggg ttgatgccca tgaggcggaa ggcattgcgg
     1741 tacgggacga tatgcgggtc ttctttcagg ttgataccct ggaaatcatc ggcaaaggca
     1801 gcaatggcct ggtccagccg gtcggcaacg gccggattgt cctgacggtt caccaggcct
     1861 tcagcggcga cgactccgac ataatagtcg ggaagcatgc gaaagacgtc ttcatcgata
     1921 tgaaatattt tcatgatagt tcctcctgtt cacggaatgt tttctcattc actaccggaa
     1981 tcgtatcata gataaagatc gttgtgaaat gaaagacgcg tcattttcaa tcactttttt
     2041 tcatggcgtc agccatacag acagatggcg tcagatatgg tatagtaaac ttatcaatgc
     2101 gaaggaggga cgctcatgaa aaatgcgtac atcaaattca tcctggctgc tgtcattttc
     2161 ggcacgaacg gcatcatcgc cagcgatatt cccgtgtcga gttttgaaat cgtcctgacc
     2221 cgtacgggcc tgggcggttt tttcgtgcgc atcctggcga cggcccgccg cgaatggggc
     2281 agcctgcgcc gcgcgtcccg cacgtccctg gtctggctgg tcctgtcggg cctcttcctg
     2341 tccgggaact ggctcttcct ctacgaagcc taccagcata tcggcgtcag cctggcaacc
     2401 ctcatgtgct actgcgggcc catcctgatt atgatcctgt cgtacttcgt cttccatgag
     2461 ccctttacgg tcccgaaagt aagcgccatg gtcatcgtta cggtcggcat cctctgcatc
     2521 aatggcgccg acgttcaggc gcacggcctg tcgtggggcc tggtctgcgg tttcctgagc
     2581 gccgtctgct ttgccctgct ggtcattgcc atgaaaaagg tcagcggcat cggcggcatc
     2641 ctctccccgg cctgccagct cctggtggcg tcgctcgtcg tcgccgccgt cactttgtcc
     2701 atgccgacgg caccggccag cctggatatg accaacatcg cctgcatggt ctgcctgggc
     2761 atcgtcaaca cgggcctcgg ctactatctc tatttttccg gcgtccagcg cctgtcggcc
     2821 cagagcgtct ctatctgcgg ctacatggaa ccctttacgg ccctggccct gtcggccatc
     2881 ctcctcggcg aaagcctgac cttcctgcaa tggatcggtg ctgcctgcat cctcggcggc
     2941 gtcgccctca gcgaattatg gcatcccggc aaaaagggat aaaaactttt tcagctcgtc
     3001 tgtcatgccg tcctagcgat aagccaggac ggcatgttct gtaaagtcgt cgcccagggg
     3061 gatgacgctc aggccgggat aggcatcgct ttcgggcatg ggcaggatgg caaagccctg
     3121 ggacaggctg accgtcagca gctgttccga cagggtctta cacaggtgga ccgacaggaa
     3181 ttcctgatgg aaggcgtggc ggcagtgctg gcgtatcttg gcggcatagg ccgggctgtt
     3241 ggatttggtc acaaagggac tgtgggcgat gatgtcgcct accgtaccgt agcgttcgag
     3301 gaggggcttg gaacagatga aggactggcg gatggcgaat aggcgctggc aggtgaagcg
     3361 gcccatcagg gattgggcat cggaaaagag gaagccatcc aaggtcccgt tctccaggct
     3421 ctcttccaat tcctcgatgt cgtggaaact gaaatagagc ttacagtcgg gaaaggcttc
     3481 ttcaaagcgg cgcagccaca gggcttcgat gtattcctgc atggcaatgc ccaggtagag
     3541 gcctttccgc cgtttcaggc gcaatgagtc gcgcaggtcc tgatattcct ggcagatctt
     3601 cgccatgcct taatagcagt gccgtccttc cggtgtcagc gagacaccgc ggtgctcccg
     3661 gcggaagagg cggcagccca attccttttc cagctgggta atgtatttcg tcatcgtcgt
     3721 ctgggcgatg tggcaattcc tggcggcctg ggtgaaattc gtcagccgtg ctgcttccag
     3781 gaaatattgt attttcgcaa ggtccataat cggtcttcct atccatcact ttttttcatg
     3841 gccatggtca aaggtttcat tagtcatgcc gccaccagca tgataaaatg gggacgtttt
     3901 agaaaggagg ctggccaatg gcctattttt ccattgtctt atcgcaaatc atcggtttcg
     3961 tcatctatat cctcatcggc atcgccgccg tgcgcatgcg cgtcctgggc agccgttccc
     4021 tgggcgtctt ctcgcggttc atcaccaaga tttccctgcc cctgctctta ttctccaaca
     4081 ccctcaacgg ggcgacgcgc cagcagttca tcagcgccct gccggtcctg gccgtaacgg
     4141 tcctgatgta cttcttatta tacctgctct gctgcctttt ggcacgcctc ttcggcctga
     4201 aaggcaatga aaatcacgtc taccgggcct gtgccatgtt cggcaacatc ggcttcatgg
     4261 gcatccccat cgtcgccgcc gtcttcccgg aacaaggcat gctctacatc gccctgttca
     4321 cggtcatcga ccagctcctg ctctggacgc tgggtgtcaa cctgacggca ccggtggacc
     4381 gggaaaatgc catgtccatc gccagccgcc tgaagaggat ggtcaacccg gcgaccatcg
     4441 caatcgtcca ggccatcgtc gggannnnnn nnnntcttta cgggcatcga gctcccggct
     4501 ccggtcaata cggccctgac caaagccggc gccctgacga cgccgctggc catgatttac
     4561 atgggtggcc tgttctgcta catcgacatc ccccgctata tccgcaagaa agagttctac
     4621 ggcgccgtcg ccatcaagat gtgcctcttc ccgcccctct tctacatgct ctgccagacc
     4681 ctgggggtca tccacgacgt gacggtcacc atgagcctcc tgtcggccct gcccagcatg
     4741 gccgccgtgg tcatgctggc ccagaaccag cactcagccg gtaactacgc cgccggcatg
     4801 atcctggtca cgaccctctg ctccatcgtc accctgcccc tggtctgcct gggattggga
     4861 tgaaaaaagg gattgtcgca catgcgacaa tcccttttaa gttgttagtg gctagcagtt
     4921 agttgttagt agatggttta aaatttaaag gttttcgctg aaaactacaa actccgtact
     4981 acaaactata gagcgaaaga atccctgcat gatggcagaa gccttcacgc aactcaggca
     5041 tccaattttc ccgtgatgag gtagactaac aggccttttt cggcgtggcg tctgttttcg
     5101 gcttcgtcga ggatggtttc caggtgtttt tccatgacgt cctgcgtgat ttcatagccc
     5161 ggatgaatcg gcatgtcgtg gaagaccatg gtatccgtcc cttccatgag ggcggcgtcg
     5221 atctgataag gcatcatcat gttgatgatt tggttcttct tttcggcata gtccggattg
     5281 gtgaagaatt ccatgtcgac ccaggtgtcg gtatagacgc agtccacgtc ggcgacgact
     5341 ttcttcaggt cttctttgga aatgtctgtc ggcagttcct tataatggcc cgtagccgtc
     5401 agtttgtcgt agaaatcggc cggcacattg gtaatctgac tgaacggcgt caggccgtac
     5461 atcatgccgc ccatcttggt gacgatttcg ctgagcgagt tatacgtatt gttcttggcg
     5521 ccgatgtaga ggaccttggc ttcaaaatgg ccggtctttt cgtagagcgt cagcatgtca
     5581 gccagggcct gcgtcgggtg gaacgtattg tcacagccgt tgatgaccgg gaccgtggcg
     5641 ttcttcatga agccttccgt cgtttcgtct ttcttgaacc ggcccatgat gcagtcgacg
     5701 ttgcggcaga cgtatttgac ttcactcatg aggtcgccga tagtgaaatt gctgtccttc
     5761 cacagctggt tgtaggcttt gccgcctaat tcttccatgc cgatggaaaa ggaaaggtac
     5821 gtccggttcg acgtcttttc aaagagcgtg tacaatttct tgccatccag gatatggccg
     5881 taatcatctt gatgctgctt gatcttttcg gccagcaata aaatgccttt caattcatcg
     5941 gttgtataat ctgcgaagct gtttgcatgt ttcatgtata tccatcctcc aatagttaag
     6001 accagaactt gttcgatgga tatatcatag cacagccgta tgaatattgc aagtatttat
     6061 gcaaactttt tcgtgttttt atacatatac aaggtgcaaa aaaaagggcc gcgcaggctg
     6121 gccgaagcct tcctgtgagc cccttaaaag gtctatttta ctttccgatt agcatttaac
     6181 tatgttagct gcctgcggtc cgcgcgcacc ttcaacgatg tcgaattcaa cttcctgacc
     6241 ttcggtcaag gatttgaagc catcttcctg gatagcggag aagtgaacga aaacgtcgcc
     6301 gccatcttct ctttcgatga agccataacc tttttctgcg ctaaaccatt ttactttacc
     6361 gtgcataaca aaatcctcct aaaaaaacaa atccagctat cttgtagctc ttacgttagt
     6421 ataacataac ggattaacaa tgtcaatcga aacggcggac atagtggacg aaagaaaaag
     6481 aatatttttc ccgtccgtct ggacggtgca attcccggtt ttgttcgtcc cactcgtcct
     6541 ttttccaggc cgggaagaaa gtgtcggcgc cgtcgaaggc ttcgtcgatt tccgtcaggt
     6601 acaggctgtc ggccaggggc agaaaggctt cggacatgga agcgccgccg atgacgaaat
     6661 agtcctcccc ggcttccatg gcctgctgca tggcttcgac actgtgga
//
LOCUS       DAWCYS010000069         6706 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_700_length_6706_cov_5.657044, whole genome shotgun sequence.
ACCESSION   DAWCYS010000069 DAWCYS010000000
VERSION     DAWCYS010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_6706_cov_5.657044"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1373 a         1922 c         1936 g         1475 t
ORIGIN      
        1 cccaaagggc gggccgcagg tcgcaggtcg caggccgtgc ctcgtggatg gctttaaatt
       61 tagtcccgtc tgctaaaaac tacaaactgc aaactaccaa ctattacggt acgatggata
      121 cgcggacgtc gtagaaggtg ctgccggcgc cttcgtcggt caggcgctgg gacgtgagga
      181 cgttgactga ggctccctgc cctttgaccc gttcccgcca ccagacgcct tcgctgacga
      241 cctggcccgg cacagtccgt ttagagatga cgacggtgaa atcggcctgg ccccgttcat
      301 tggccaaggt cacggtctgg ccgtcctgca ggccatagcg ggccgcatcg tcgggatgga
      361 cgaagaggtc cattgtatgg ctggccgtca gttcgtcccg ttcattgaag gacgaatcga
      421 ggatgcgcat gtccggcccg ttgacgaaag ccagcgtgcc gtcgtcttct gcaaggggca
      481 gatagtccgg cagggccggc gtcagggccg cgttgtatat ctcgattttc cccgacggcg
      541 tggcaaatcg cgtctggtaa tcgtccggca tgggcagcgg cgtcggcagg ccctgactaa
      601 ggcgctgctg atccacaggc aggggccatt tcgtcgtcga cgcagccagg gcttcgacga
      661 ggtcccgttc tgagcaatcg tagaaatcat cgtctatacc catagcatgg gccaggtcac
      721 aggcgacgcg ccagttggag cggctctggc cgacaggcgg gatggccgca tagccgcact
      781 ggatcgtgta gttgccatag gaataatagg cgtcgtcgtg ttccagcgac gtcgtcgccg
      841 gcaggacgat atcggcatag cgggccgtat cggtcataaa gcgttcgtgg acgacggtaa
      901 ataaatcgtc ccgacagagg cccttcagga cctggttctg gtccggcgcc gtgcaggccg
      961 gattggacga atagacgaag aggctgcgga tgcggtcgtc acccgtcagt gcctggccca
     1021 ggcagcacat gttgacgacg cgcttgccag gctgtttcca atcggaatgg gtgacgattt
     1081 ctttatcata ggctttcgac gccgacgccg aagtcaagag gccggctccc tgcttccccc
     1141 aggcgccgac gacagccggc aggcaggtga tgaggcgcgt cgtcatggcg ccgttgcgat
     1201 agcgggactg gccgctgccg aggcggatga acggcgcccg ggcccggccg taggcgtggg
     1261 ccaattcgac gatgacgtcg gccggcacgc cggtgatgcc ctgcgttttt tccggcgtat
     1321 acttggacag gacgttcttt ttcaagtcct cccagccgag gacgttccgg tccaggaagg
     1381 cttcgtcagc caggccgtca cggtcgagga catgcatcat gcccagggcc agggctccat
     1441 ccgtaccggg gcggacggcg atgaagtggt cggccaggtc ggccgtcttg ttgcgatagg
     1501 catcgataca ccagacgacg gcccctttgg cccgggccgc ttcgacgtcg tggtggaagt
     1561 ggatattcgt ggccagcata tggatgctcc agaggaagat gcagtcgctg tcctgggctg
     1621 cctgcggcgc cgtcggcatg gtattgccca tgacgtcctt gaagccccgc cctttggccg
     1681 gagcgcagat ggtccggtcc agttccgaag cacccaaacg atagaacagg ccgtgcaggg
     1741 cgtcgtgctg gacgatgccc atggtaccgg cataagaata gggcaggatg gcttcggcac
     1801 cggcttcggc gatgatggcc ttccaggtcc cggctatttc tgccagggcc tggtcccagg
     1861 aaatgggcgc gaactggccg cttcccttgg ggtcaatccg cttcagcggc gtcgtcaggc
     1921 ggcgcggcga atggacggtc cgctcataat gggccatctt cgggcacaac gtcccgcgcg
     1981 taaagggatg ggacttgtcg ccggcaacgg atacgacgcg gccgccgtcg accgtcacga
     2041 cgagcccgca ggcatcgggg cagtcatacg gacagacaga tgtacaggtt ttcatggtat
     2101 cacctcttgt ttcgtggaaa aaaggagctg tcgcatcgcg acagctcctt ttaagtagtt
     2161 agcagttagg ggttagcagt tagcagatgg cataaccctt cgtgcgacag cgccttttct
     2221 tgtaggggcg ccacgtgggg cgcccgctct gggataggta cgatttcccg acccgtttgc
     2281 tagaagaatc atccaaattc ctaacaggtc tcccacgtgg cccctacaaa ctatagagca
     2341 taggaacccc gcgagcggtc cttcttcttc ccaggggaag ccttaatgat ggaacaggcg
     2401 caggccggtc atggccatgg cgatgttgta tttatcgcac gtttcgatga cattgtcgtc
     2461 gcggatggag ccgccggcct gggcgatgta gtcgacgccg ctgcgatggg cccgttcaat
     2521 gttgtcgcca aaggggaaga aggcatcgct gcccagggct acgccgtgga gctgggcgat
     2581 ccaggctttc ttttcttccc gtgtgagcgg ttccggtttt tccgtgaaca agtcctgcca
     2641 gatgccgtcg gccaggacgt cttcgtagtc atcggagatg tagacgtcga tagcgttgtc
     2701 gcggttcgga cggcggacat cgtcgcggaa aggcaggttc aggactttcg ggttctggcg
     2761 gagccaccag atatcggcct tgttgccagc caggcgcgtg cagtggacgc gggactgctg
     2821 gccagcgccg acgccgatgg tcatgccgtc tttgacgtag cagaccgaat tggactgggt
     2881 atatttcaaa gtgatgaggg cgatcatcaa atcgcgctga gctgctgccg ggatattctt
     2941 gttcttcgtc gggacatttt tgaagaggtc cgtcgtaatc ttggcttcat tgcgttcctg
     3001 ttcaaaagtg acgccataga cttccttcga ttcgacgagg cgcggcttgt agtccgggtt
     3061 tatctggagg acgcagtaat tccccttctt cttggtcttc aaaatagcca aggcttcgtc
     3121 gctgtagccc ggagcgatga cgccgtccga gacttcccgc ttgaggagcg ttgccgtttc
     3181 gacatcgcag acgtcggaca gagcgacgaa atcgccgtac gagctcatgc ggtcagcacc
     3241 gcgggcgcag gcataggccg aagccagggg ggacaatttc aaatcgtcga cccagtatat
     3301 ctttttcagg gtatcactca tgtccgtagc gacggcggcc ccagccgggc tgacgtgctt
     3361 gaaggaagcc gctgccggaa ggcctgtcgc ttctttcaat tctttgacca gctgatagct
     3421 gttgaaggcg tcgagcaaat taatataacc gggacggccg ttgaggaccg taaagggcag
     3481 gtcgctgcca tctttcatat aaacccgggc cgacgactgg ttgggattgc agccgtattt
     3541 taattgcagt tctttcatgg tgattcctcc tgaatagtta agcaaaaata aaaaataccg
     3601 acacttctgg aacgtttcgc ccagacggtc ggctcgtacg aatcatgctc cccgtggtaa
     3661 ctccacttcc gtcagtcaca tgtttcagat ttattatacc gtccgcagac gggagatgtc
     3721 aagaaaaagg accgcccttc ggacggtccc tttttctgta ggggcgccac gtggggcgtc
     3781 cgctccagga tgatgtgatt tccatttccc attcggttta ctgggagaaa catccaaatt
     3841 ccgaacgggg cgcccacgtg cgccccctac agtgccacta tccactaacc cgcctgttgt
     3901 tcaaagatgt cctgggccag gcccatgggt tcatcgcgga agaggcgttc atccatggtc
     3961 ttcagatttt cggcgatgtg cggtacgaat tccatctggt cgaggatgtc cttttgcaga
     4021 tccacgcccg gtgcgatttc cgtgaggact aagcccttat cggtgaggac gaagacacag
     4081 cgttcggtga tgaacatgac ctgctggttg tgtttatggg catattcagc actgaaggta
     4141 atctggccga ccttatcgat gaatttctgg acagcccctt catgggtgat gaccagtttg
     4201 ccgtcccgga tttcttcgcg caggcccttg gccgtaaagg tgccgcagaa gacgacttta
     4261 tgcgtattct gggtgatgtt gatgaagccg ccgcagccgg cgatgcggtc tttgaacttg
     4321 ctgacgttga cgtcgccttt ggcgttacat tctgccatgc ccaggaaagc catatccaaa
     4381 ccaccgccat cgtaaaagtc gaactgatac ggatggtcga cgatggcatc ggcgttggcc
     4441 gaaccgccaa agtccgcgcc gctggccggt acgccgccga taggcccggc ttcgacggtc
     4501 agggtcagct ggtcgccgat accttcttcg ttagctacgc tggaaacacc ttcaggaatc
     4561 ccgatgccca ggttgacgac ggcgttggga acgagttcca tcgcagcccg acgggcgata
     4621 accttgcggg cattgagttc catcggcgga atctgggaca aaggcatacg catttcaccg
     4681 gtataagccg ggttctggac ctgacggacc ttggacaaga cgatgtagtc gacgtcgatg
     4741 cccggcagga cgacgtgttt cggcggaatc gtccggtttt cggccagcgt ttcgacctgg
     4801 acgatgacca tgccgccact ggctttggct gccagggcca tgggcaggag ttccatgcgg
     4861 acttcttcat tatccgtcga tacgttgcca ttcaagtcgg cgcaggtccc gtggacgata
     4921 gcgacgtcga tggggaacgg tttgtaccag agatattctt cgccgtccag ttccttgact
     4981 tctaccaggt cttccttagt gatgctgttc aatttcccgc cttcgatgcg ggggtcgacg
     5041 aaggtattga ggccgacacg ggtaaacagg cccggtttgc ggccggcaat gctgcggaac
     5101 cactgggcca tcgtgccctg cggcaggttg tagccttcga ccttattgtc catgaccagt
     5161 ttcccaagtt tgggagccat gttgtaatga ccgtcaatag accgtttcag gatgccttcg
     5221 tgggcgaaat ggctggcacc cttgctcaca ccgtcaccgc tgccggccgt aaggaccagt
     5281 gtcagctgct gcggatatcc ggtcttggta aagcggtctt ccagggcctg caatacttct
     5341 tccggcaatg cacactgtgc aaaaccacaa acggcgatag tcgaacgatc tttaataagc
     5401 gatgctgcat tttctgctgt aatcagtttt gccatgatga cttcctcctt atagatttcc
     5461 ttacttgtct tcgatgagct ctttttcttc tcatcttctg gctttagtat agcccctttt
     5521 gcccttgaaa aatacgcaca aagccgtgac aaaagatgaa aaacttgcct caaagatgac
     5581 aaatatgaca aggcctttcc ttgaaggtgt cctggccagg ctatataatg aatataagga
     5641 atataaggaa tatacacgta ttcaaatcta tcatcaaggg agtcctgttc atgaatcctt
     5701 atgcagaagt cctcaagcag ctgctgcaat atacagagac gaaatatacg accttatccc
     5761 aggccatcga ctacgacatg tcctacatca gcaaatgggt caacggcgcc cgcctgccgg
     5821 cttcacgcca tgccgaccgc atccacgagg acatggcccg cttctttgcc aacacggccc
     5881 agaaacagca ccaggaaggg gacctgaagc gcatcttctc ctttgccgac gacggccagg
     5941 atctggcctt ccatctctac gacctgctaa gccgtgccta ccgccaggcc cagaccagga
     6001 aggggaaaag tgaaagccgc gaccaggcca tccatttcta cgccggccgc gctgactgcc
     6061 acacggccct gtgccagctg ctccaccaat acctggaagg cctccagtcc cagggaatgc
     6121 tggtcatgac cggccagttc cacgtcctcg accagaacgg catctggcag ctcctggaag
     6181 aatgtcacaa cgaccgctgg tcctgcgacg tccacttagg cctgaagatg gacgtctttg
     6241 ccgatcatat gccccaggcc gccagcagcc tctatcagtg tctggaccac ttattgacct
     6301 ataatttcac catgcacgaa atcgaccacg aatcgtaccg caacatcatc gtcctcaaag
     6361 acgcctttgc ctacctctac tacgtcgacg gcgacgacta catcgacatg tgcctgacca
     6421 tcaccgaccc ggcccaggtc cgggaaatct ataagaagtg ctgcgcctac atgatggtcc
     6481 agcccgtcct catggagccc cgccagtccc tgggcatgga aaaattcggc taccgcgaca
     6541 tcttctttac atccaacaac tacctcttct tcctgaccaa cggcctggaa ttcctcctcc
     6601 ccgacgaagt ctatacgtcg ctgtgccaac tcatcgacga aggaaaatat cccaatgcca
     6661 ccaaagaatg ggtccggcgc atccagtaca tctggtaccg catcat
//
LOCUS       DAWCYS010000070         6640 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_705_length_6640_cov_5.042369, whole genome shotgun sequence.
ACCESSION   DAWCYS010000070 DAWCYS010000000
VERSION     DAWCYS010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_6640_cov_5.042369"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1567 a         1989 c         1781 g         1303 t
ORIGIN      
        1 agccaacttc aacgatttgg tcagccagac ggcggccctg gtgacgcagc ttgaaaatga
       61 gcagacccag ttctatatcc cggaccaggt caagaaaatc gtcaacgatg ccttcgtcaa
      121 gatcggcaat tacggcatcg agggcatcac caacctcatc cagtccgtct ttgccattgc
      181 cgggacggtc gtcgaattct tcgtcgtacc gatcattact ttctacttca tgaaagacgg
      241 cggccacatg gtcaagagct tcgtcgatat ctttcccgaa agctatcgca gccatctggc
      301 cggcctcttc caggaaatac agcacgtcct gagccgctac atccgcggcc agatcctcat
      361 gagctgtatc attgccaccc tgactttcct cggcatgtgg gccatgggcg taccgtaccc
      421 catggtcatc ggcatcttgg cggccattac cgaatggata cccatcgtcg ggcctatcgt
      481 cggcgccgtg ccggctatcc tgctcagcgc gacggtcagt ttgtccctgc cgctgaaagt
      541 catcatcttc tatatcgtca tccagcagat cgacagccac ctcatcatgc ctcaggtcat
      601 gggggccgtc atcagcctgc atccggtcgt catcgtcatc gccctgctca tcggcgggac
      661 cctctttggc gtagccggca tgatccttac cgtcccggtg acggcagtct tacagatttt
      721 gtgtaaacat ttatggtttt acaatacata taaagaaaag gcgatgaagt acaatggaaa
      781 caacgagaac agaaaccaat aagaaagaaa tcatggatac catgatgaaa cgcatgaaag
      841 aaaagatcaa gttcaagaaa tgcaagatga cgtcccagcg gcagatcatc ctgcgggcct
      901 tcgtcgaaag cccaatccgc catatgagcg ccgaagaagt cttcgagctg gtcaagaaga
      961 cggcgcctga cctcggcctg gctacggtct accggacgct ggacctgttc acccagatgg
     1021 acctcttgaa gaaactcgat ttcgacgacg gctgcagccg ctatgaattg aatgaccgcg
     1081 acaatgaagg ccatttccat catcatctca tctgcctggg ctgcggcaag gtctgggaat
     1141 gtgaagacga cctcctggaa acgctggaaa cgatcctgga aaagcgcctc cacttccaca
     1201 ccgtcgacca tcagctgaaa gtctatggct actgcgaagc ctgccagaag aaacgggaag
     1261 aagaagcggc caaagaagcc gaagctgcga aagaagcgga ataagatggc cctccagacc
     1321 tatacctatg acgggccgga acgcttccat tccctcgtcg ggcaggtcat ggcctccctg
     1381 ggctatgtcg gcggccggga agtccgcgac gtcggggctg ccgtcactgt ccgcgaggaa
     1441 ggcgcccacc tccacgtgcg ctgtaccttc caggacggcg gggcgccctg gtcttctgag
     1501 gcccgcctcg tcgggacgga ccgggaacgg gcatccctca aggactgcct catccactgg
     1561 cataaccgtt tctgcggcca cggacccagc ccgtggggga cgctcatcgg cgtgcggccg
     1621 acgaaactgg tccaccacct cttcgaccag ggcttcacag ccgaccaggc cgaaagggcc
     1681 ttgcagacgt cgtattgcgt ggcgccggcc gtcgcccggg acctggtggc catggccagg
     1741 ctccaacggc cttacgtcac cgacaggggc cggaaagtgg ccctctacgt agggattccc
     1801 tattgtccca gccactgcct gtactgctcc ttcccgtcgc gcctcatcgg cagggaaggg
     1861 gaggcggaac tcacggcttt tcgcgatgcc gtcctggccg acctcgacga cctggccgac
     1921 ctgtgccgga cctatgactg ggaaggcacg tcgctctacg tcggcggcgg tacgccgacg
     1981 tgcctgcccc tgccggtatt ggaagcgatg atgacgcggc tggcccggaa tttcccggaa
     2041 gcctgcgaat ggaccgtcga agcgggacgg cccgatacgg cgacgccgga catgctggcc
     2101 atgctccgcc aggccggtgt cgatcgcatc agcgtcaatc cccagacgtt gcagcagcat
     2161 ctcctcgatg ccctgggccg gcgccaccgg gtcgaagata tttacaccat gtatgagaac
     2221 tgccggaaac tgggtttttc cgtcatcaac atggacttta tcgccggcct tcccgggcaa
     2281 accgtggcag atatgcagga aaatatggaa attgtttgca aactgcaccc ggaaaatgtt
     2341 acaattcata cgttagcatt aaaaaaacgg gcacccttat tccatcatga attgcgggcc
     2401 gccattccgc cagccgaaga aacgggccat atggtccgcc tctgtcagtc catcctgacg
     2461 gccggcggct atgtgccgta ctacatgtac cgccagaagt acatggcagc cagcttcgcc
     2521 aacattggct atgccctgcc gggcagcgtc agtgcctata atatcgagat gatggaagaa
     2581 cggcagtccg tcctggcagc cggaccgggc ggggcgacga agttcctctg ccgcgacggc
     2641 cacaccttgg aaaaggtgta catgctgaaa gacgtcgatg catatgtgca ggcgctggcc
     2701 gaaagaatag cccagcgccg ccgtttgtgt gctatcatat acggaaaaga ggatgtttaa
     2761 atggctatta ctgcaccgag aggcacgcag gattttctgc cggaacagac ggcagagtgg
     2821 cagctgctgg aagcaaagat ccgccagatc tgctcgctct acggcttcgg cgaaatccgc
     2881 acgcctatgt ttgaaagtac tgaattgttt ttacgcggca tcggggaaac gacagacgtc
     2941 gtcaccaagg aaatgtatac ctttgaagac cgcggcggcc gcagcatgac attgcgcccg
     3001 gaaaatacgg cttctgtcgt ccgggccttc ctggaacaca agctctacgg cgacccgcag
     3061 gtccacaagt tctactacat gggccccatg ttccgtcatg accgccccca ggctggccgt
     3121 taccgccagt tcacccagtt cggcgtcgaa gaaatcggct ccaaggaccc ggctgtcgat
     3181 gcggaaatca ttgccatggc cttccagctc ttccgggaac tgggcctcaa tgatctggtc
     3241 ctgcacctca actccgtcgg ttgcccgaag tgccgtcccg tctatcgcca gaagctcctc
     3301 gatttctttg ccgacaaacg ggaccagctc tgcgacgact gcaacgaacg cctggaaaag
     3361 aaccctctcc gcgtcctcga ctgcaaggaa gacggcgaaa agatgatgca gatgggcgta
     3421 cctttgatca cggataatct ctgcgacgac tgcaaggaac atttccacaa agtccaggaa
     3481 tatctgacgg ccatcggtat tccctttacc ctcgacccgc gcctggtccg cggcctcgat
     3541 tattatacca atacggcctt tgaaatcatg tacgctcctt tgggcgcaca gagcacggtc
     3601 tgcggtggcg gccgttacga cggcctcatc gaagaagtcg gcggtccgtc gacaccgggc
     3661 atcggctttg ccatcggcat ggaacggctg ctcctgccct tgcaggacca gggcctcctg
     3721 ccgccggtcg agaagaagca gcgcgtcttc atcgtcgccc ttggcgatgc ggccaagacc
     3781 agggccttcg aactgcagca gcagctccgc gaaaaggacc tctatgctga aatcgacctc
     3841 ctgggccgca gcatgaaggg ccagatgaaa tcggccaaca agctcaatgc cgattacacg
     3901 gtcatcatcg gcgaaaacga attagccgcc ggtgaagccc aggtccgcaa tatggccagc
     3961 aaagaacaag tcagcgttcc ccttgatggg attgttgcat atatggaaac acacaagaag
     4021 ggatgattta cgaatggata caatggaagg tttacaccgc tcccaatact gcgctgaagt
     4081 aagtgaagcg gacaacggca aagaagtcgt cttgagtggc tgggtcgaac gccgccgcga
     4141 ccacggcgga ctgattttca tcgacctccg cgaccgtacg ggcatcgtac aggtcgtcgc
     4201 ttcgccggac tatgaaaagg tttctttcga taaagctgaa caggtacgta cggaatacgt
     4261 cctggctgta cgcggcatcg tccgcatgcg cgataaagat acgatcaacc ccaagatgaa
     4321 gaccgggacc atcgaaatcc gctgcgaaga actgcgtatc ctcaattcgt ccaagacgcc
     4381 gccgttctat attgaagaca acatcgacgt cgacgaaaag atccgcctca aataccgcta
     4441 cctcgacctg cgccgtccgg aaatgcagaa gaacctcatc atgcgccata aagtaaaaca
     4501 tgccatgcgc acgttcctca acgaacacgg cttcatcgac atcgaaacgc cggaactctg
     4561 caagagcacg ccggaaggcg cccgtgacta cctcgtaccg agccgtgtca acgaaggcaa
     4621 attctacgcc ctgccgcagt cgccgcagat tttcaaacag ctgctcatga tttccggtat
     4681 ggaccgttac taccagatcg cccgctgctt tcgcgatgaa gatttgcgcg ccgaccgcca
     4741 gccggaattc acccagctcg acatggaaat gtccttcatg gaccaggaag aaatcctgac
     4801 cctcgtcgaa cagatggtac attacatctt caaagaaacc ctgggccacg acgtcaagct
     4861 cccgattcac cgcatgacct gggactacgc tatggaaaac tacggctccg ataagccgga
     4921 tctgcgcttc gacatgaagt tcacggacgt cacggaactc gtcaaggaca cggacttcaa
     4981 agtcttccgc agcgtcatcg acaacggcgg tcaggtcaaa gccatcaacg tcaaaggcga
     5041 cgccgacatt ccccgtcgtg aactggacgg cctcgtcgaa tacgtcagcc actacggcgc
     5101 caagggcctg gcttggatcg gtttcctcga agacggcacg ctcaaatcgc agatcaagaa
     5161 gttctttggc gaagacaagc tccgtgaaat cggtaaagcc tgcggtgctg aaaagggcga
     5221 cctggtcctc atgattgccg acaaaccgaa agtcgtcgca gccgctctcg gcgaactgcg
     5281 taaagaaatg gcccgccgca tgaacctcat cgacgaagac gaattcgcct tcacctgggt
     5341 cgtcgacttc cccatgttcg aatacagcga agaagaacac cgctacgtcg ctatgcacca
     5401 tccgttcacg gccatgcgcg acgaagacct ggacaaactg gaaaccgacc cggctcacgt
     5461 ctatgccaag gcttatgact tggtcctcaa cggcatcgaa ctcggcggcg gctccctccg
     5521 tatctaccag cctgacgtac agcagcgcgt cttcaaggcc atcggcctga caccggaaga
     5581 agcagaacag aaattcggct tcctcctccg cgccttcgaa tacggtgcac cgccccacgg
     5641 aggcctggcc ttcggcttgg accgcatgat catgctcatg ctcaaacgca agtccatccg
     5701 cgacgtcatc gctttcccga agacccagaa cgctatcgac cccatgagcg aagctccgtc
     5761 gacagccacc aaaaaacagc tgcatgaact ccacatccag gtagaagaag acttggtaag
     5821 caaatagaga gatgaaaaaa gcttgtcgca tgtgcgacaa gctttttttc gtggcgcggt
     5881 cctatggacc gctttcgtag gggccgtcct aagccttttt ggcccgtcag gtgccgacca
     5941 acgagaggcg caggacggtt gccaaaacgg acagctgtgg ctaggcggcc cgcttgtggg
     6001 aatcccgtga atggctttcc caaatgaacg cgattggttt acgaaccacg agccgctagc
     6061 agacacattg acaaacgtca atgtgtctgc tatactctaa ccatagattg ataaacttcg
     6121 atatgaaagg aggcgccgtg atggaggaaa gggaagcggt tcgcgtattc aaggctttgg
     6181 gtgatgaaaa gcggctgcgc atcttgttgc tcttgcggca gggggaacgc tgtgcctgcg
     6241 tgctcctgga acacttgaac ctgtcccagc cgacgctgtc ccaccacatg aagatcctct
     6301 gcgatgccca gctggtcaca ggccgcaagg aagggaagtg ggtctattat tccctcaacc
     6361 gcgcccaggc ccaggtcctg gaacaggccg ttcacgactt attccagccc agtgccgtgt
     6421 cggcagagga cgaagcctgc cgctgccagt gaggcaggcc cttttttcag cttatacatt
     6481 gataaatgta aatatataaa tttctattta ggaggtcttt catcatgaaa catatggaaa
     6541 tctttgaaca agcaatgtgc tgcccgaccg gtctctgcgg tccgtctatc gatccggaac
     6601 tcttgcgcgt atcgacggtc ctggacacgc tgaaacagca
//
LOCUS       DAWCYS010000071         6526 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_713_length_6526_cov_10.085613, whole genome shotgun sequence.
ACCESSION   DAWCYS010000071 DAWCYS010000000
VERSION     DAWCYS010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_713_length_6526_cov_10.085613"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1607 a         1765 c         1679 g         1475 t
ORIGIN      
        1 cgccattgat gcccctacct aagaggaagg agtcttctgg cgaaactatg ataaaatagt
       61 gtaatcctct gacgaaaaaa tagtagcgaa tgcgagtata ttctctccta ttatcgcatt
      121 tttgttcatg taaatgcaag gtaaaataga aaacaaattc agaaatatga ccttttatgt
      181 atactatatt ttgcataaaa attatagcct gttttaaggc tatacgcaaa gtgaaaatat
      241 gtaaatattt agttgaaata atgtgctagg tgatgtatga tgaaatcaca gaaaatcctc
      301 tatgcaggta gcgaaagaag ataccagtgt aattgatgaa aggagcaatg ttattatgca
      361 agacaagcaa tacgtcatgg cccttgatgc cggcaccacg agtaatcgcg ctattatttt
      421 cgacaatgaa tcacatatcg tcagcgtcag ccagaaagaa ttcactcagt atttcccgaa
      481 accgggctgg gtagaacatg acgccaatga aattttccac agcatggtcg aagtcatgcg
      541 cgaagccctg gaacaggcca atatgacggc tagtgaaatc tcggctatcg gtattactaa
      601 ccagcgtgaa acgacggtcg tctgggacaa gaacacaggc gaccccgtgt acaacgccat
      661 cgtatggcag tcccgccaga ccgcgccgat ttgtgaagac ctcaaagccc gcggcctggt
      721 cgatgaattc cgtgaaaaga cgggcctggt catcgatgcc tacttctcgg gcacgaaggt
      781 caaatggatt ttggacaatg tcgaaggcgc ccgggaaaaa gccgaaaaag gcgatctcct
      841 cttcgggacc atcgactgct ggctggtctg gaaactgaca ggtggccaag tccacatcac
      901 cgactattcc aacgcatccc gtacacttat gttcaacatc aaagaactga agtgggatga
      961 ccagcttctt gaatatttaa ccgttcctaa atgcatgctc ccggaagtcc gcccgtccag
     1021 tgaagtctat ggctgcacgg atccggctat cctcggcggt ccggtcaaca tcgccggtat
     1081 cgccggtgac cagcaggcgg ctctctttgg tcagacctgc tatgaaccgg gtatggccaa
     1141 gaacacctac ggtacaggct gcttcatgct catgaacaca ggcacgacgc cgattccttc
     1201 caagaacggc ctggtcacga ccattgcctg gggccttgac ggcaaagtcg aatatgccct
     1261 ggaaggttct atcttcgtcg ccggttcggc tatccagtgg ctccgcgacg gcctgcgcct
     1321 catcgacacg gctcctgatt cggaatgggt cgctaaacgc gttcccgact cggacggcgt
     1381 ctatgtcgta ccggcattcg tcggtctcgg cgctccctac tgggatatgg atgcccgcgg
     1441 cttgatcatc ggcatcacgc gcggtactaa gaaatctcac atcgttcgtg caactctcga
     1501 ttctatggct taccagacga aagatgtcct cggcgccatg gaagcagact ctggcatcaa
     1561 actggctgcc ctcaaagtcg acggcggagc tgttgccaac aacatgctca tgcagttcca
     1621 ggccgatatc ctcggcgttc ctgtcgatcg tccgcaggtc atcgaaacga cggctctcgg
     1681 cgccgcttat ctggctggcc tggctgtcgg cgtatgggat tccaaagaag aactcgtatc
     1741 gagctggaaa ctccagaatc gtttcgaacc gaccatggat cctgaaaaag cagcagggta
     1801 ttataaaggc tggcgcaaag ctgtcaaaca tgcaatgcac tggcttagtg atgaagacta
     1861 agagagaggt gctggaatca tgatgtttga caacttatta ggagaattta tcgggacgat
     1921 ggtcctcatc gtcttcgggg ccggcgtctg cgcctgcaac acgctgacca aatcgaaagg
     1981 ccagaacggc ggctggattt gtataaccgc aggatggggt ttcgccgtaa ctctcggcgt
     2041 ttacacggca acgaccctcg gtgctccgca gggtgacctg aacccggctg ttacgctcgg
     2101 caagacgatg atcgggattt acagtgttcc tcagttcttc gccacgtcca tcgctcagat
     2161 cctgggcggt atcctcggcg cagctatcgt ctggctggct tacctgcccc attgggctga
     2221 aacggaagat aaaccggcga aactcggtat cttctgcacg gctccggcta tccgcaacta
     2281 tccggccaac ttcctttgtg aattcattgc tactttcttc ctgatgttcg ttatctggat
     2341 gattttctcc aataaagtcg gtgccattcc tcctggagtc ggcccgtata tcgtcggcat
     2401 cctcatctgg gctttgggta tgagccttgg cggtcctacg gggtacgcca tgaacccggc
     2461 tcgagatttg ggtcctcgta ttgctcatgc tatcctgccg attgccggta aaggcggttc
     2521 ggactgggct tatgcctggg ttcctgtttt cggcccgctg gctggtgcct ttgctgccta
     2581 cgtcgtagcc atggctttac atgtcttcta atttatttaa tatatggttt tgggaaaggg
     2641 cttgtcattc atcggcaagc cctctccttt acttaagttt ataaatttgt ttataaattt
     2701 tgaacataat ttcatactta atcaaaggta atattcctat atttttgcat gaaaaaatag
     2761 tataattcgg ctatgtgaaa atatgtaaaa atagtcttgc aattattttc acaaaccgat
     2821 ataataaggg catcaaagat agtgatactt ggtatttaca gagaaagagg ttgtgaacat
     2881 gacaaaacaa gcagatgtcg tcgtaattgg cggcggtatt accggcacag ccattttgca
     2941 cgaactggca aagtacaatc ttcgtgcagt cctggttgaa caggaaccgg aactggctgc
     3001 cggcacgaca aaggccaaca gtgccatcct tcacgcaggt ttcgatgcgc ctacgggcag
     3061 tatgaaagcc cgcatgaatg ccgcaggcaa tgcgatgtat catgaattga aagacgaatt
     3121 ggatctggac atccgctggt ccggctcgta tgtagccgcc ctcgacgacg aacagatgga
     3181 cgtcctcaag gaactcctcg aacggggcaa tgccaatggc gttcccggac tgaaaatcgt
     3241 cagcggcgat gaaatgcgca aagaagaacc gaatgtcagc aaggacatca aaggcgcact
     3301 ctgggctccg actgccggca tctgctggcc tttcggcctg gccctggctt ttgcggaaaa
     3361 tgccgtcatc aacggcgctg aagtcatccg tgaatgtcag gttacgggca ttaccgtaga
     3421 agacggtgcc gtcaaagccg tcgaaacgga caagggtact atcgaaacga aatacgtcat
     3481 caatgccgcc ggcgtccatg tcgacgaaat cagccgcctt gccggggacg atacctttaa
     3541 gatccatccg cgccgcggcg aatacatcct ctttgataaa acggctcaga aagacctcgt
     3601 ttactcgccg attttcccga caccgacgaa aatgggcaaa ggcatcctgg tctgcgcgac
     3661 gacacatggc aacgtcttcg tcggcccgaa cgcccaggat atgccggatg aagaaaaaga
     3721 cgacacggcc gtcaccattc cgggcatgga cgacatcctg gacaaagccc gccgtctggt
     3781 cccgaacatc cctgtaggcg ctaccattac ggaatttgcc ggtgtccgcg ccgtatccag
     3841 cacgggcgac ttcatcctcg gcccgtcgga aaagacgaaa ggcctcatcc aggctgccgg
     3901 catccagtct ccgggcctga cttcggctcc ggctatcgcc aaatatctcg tcgacggcat
     3961 cgtcgctgaa accggtgcga cagccaagac ggactataag aaaggccgtc cggctcagcc
     4021 ctgcttccgc atgatggctg aagctgacca gaaagccctc atcgccaagg atccccgtta
     4081 tggccgcgtc atctgtcgct gcgaaacgat tacggaaggg gaaatcgtcg atgccatcca
     4141 tcgccccgtc ggtgcccgca ccgtcgacgg tgtcaaacgc cgcacgcgtg ccggcatggg
     4201 ccgctgccag ggtggcttct gcggacctcg tgttacacag attttgtccc gtgaattgaa
     4261 catccccgtt acggaagtac ggaaagaaat gcgtaattct tacatgttct acaacaaaga
     4321 aagcggaggt aaggcataat gggtgaacaa ctttatgata ttatcatcgt tggcggcggc
     4381 ccggcaggct tatccgctgc ctacagcgct tggcagaacg gcgctaaaaa aattctcgtc
     4441 ctcgaacgtg accgcgaagc tggcggcatc ctccagcagt gcatccacaa tggtttcggc
     4501 ctccatcact tcaaagaaga actgaccggc ccgggctacg cccagcgctg ctatgacctg
     4561 gtcaaggata aaccggaaat cgaaatcctc gtcgacacca tggtcctcag cgtcaatctc
     4621 gacaagacgg ttacggctgt cagcccggct cacggcctga tgaaagtaca gggcaagacg
     4681 atcatcctca ccatgggctg ccgcgaacgt acacgcggtg ccatccgtat ccccggttat
     4741 cgtccggctg gcgtctttac ggccggtgca gcgcagcgca tggtcaacat ggaaggctat
     4801 ctgcccggta agaaaatcgt catcctcggc agtggcgaca tcggcctcat catggcccgc
     4861 cgcatgaccc tggaaggctg taaagtccag gctgtcctgg aaatctgccc cttctcgaac
     4921 ggcttgaccc gtaatatggt acaatgtctg tatgactatg atattccttt gtatctgtct
     4981 cataccatta cggccatcca cggcaaagac cgggtcaacg gcattacggc cgccaaagtc
     5041 gacgaccata tgaaacccat tccgggcacg gaattcgaca tcgactgcga tacgctgctc
     5101 ctgtccgtcg gcctgatccc ggaaaatgaa ttgtcccgcg gcttggggct ggctatgcat
     5161 ccgctcacca atggtcctat cgtcgaccag catcgtgaaa cgagcatccc tggcatcttt
     5221 gcagccggca acgtcgtcca cgtccatgac ctggtagact tcgtatcgga agaagctgaa
     5281 attgccggta aatacgctgc cctcaaggca caggaacagc tgccggaaga cgtagccgac
     5341 gtcaaggtcg aagccatcga cgaagtccgc acctgcgttc cccagcacgt ccgcgctacg
     5401 gcagaaggcg aaacgatccg cttgttcatg cgtgtccgca aacccatgaa gaaagtccgc
     5461 ctcactgtca agagcggtga cgatacgctg ctgacgaaag tcctccccgt cgccaaaccg
     5521 agtgcaatga tcgccgtcaa cgtcacggct gtaagagcca cgggccctca tgcggattgg
     5581 accgtttccg taaaggagga agcataacat gagtgtagaa acgaaacaac tgaactgcat
     5641 caactgcccg ctgggctgcc tgcttactgt taccctggaa gacggtaaga ttaccaaggt
     5701 cacaggcaat acgtgcccgc gcggtgaaaa atatgcccat caggaattga ccgacccgca
     5761 gcgcatgctg acctcgacgg tccgcatcga aggcggcgaa ctgccgctcc tgccggtcgt
     5821 atccaagacg acgttgccga aaggcaaaat cctcgactgt gcagcagccc tgcgcagcgt
     5881 cgaagtcaaa gccccggtcc atacaggcga cgtcatcgtc aaagatatcc tgggcctcgg
     5941 cgtagatatc gtggccagcc gcgatatggg caaagaataa gcatcggtac caatattccc
     6001 tcttacatat accagaaagg ggctgtcgca tgtgcgacag cccctttctc gttgctcgtt
     6061 gttcgtggct agtggctagc gaaaagaggt tgcaggggtc gtcctctaca gcgatcttct
     6121 tcagcagccg tcctgtcgtt ttttacagtc atgccctgac gggctgaaaa ggcacgtgct
     6181 ttagagggac tcattaattt gcttgattta ttaggcgaac atggtacaat aaaaacagag
     6241 atagtccgat ggtggtacat cggtctcctc tgtggtttat gtttgaagaa ggccgtgtac
     6301 cgcgagtacg cggttttttt catgctaaag gtttatcgct tcgttgctga aataatcagc
     6361 actataagag ttgcgaatgc aatcatcaat gacaatgctt cgtatacact catgctgatc
     6421 acctcgcttt cgcgagggaa accgaataca tcgaactatc tcatggagtt cagtatacca
     6481 gatttactac caggcttcaa ggaaaagcca ttgttcgtaa ttcata
//
LOCUS       DAWCYS010000072         6508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_718_length_6508_cov_5.969627, whole genome shotgun sequence.
ACCESSION   DAWCYS010000072 DAWCYS010000000
VERSION     DAWCYS010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_6508_cov_5.969627"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1517 a         1673 c         1572 g         1746 t
ORIGIN      
        1 acatcgccaa cgtcgcagaa gacgaagcag gcaatcccga tgccaacccc aacgtcaaac
       61 gcgtcgaaga atacgccgcc aaggaaggcg ctcaggtcgt cgccgtctcg gccaagattg
      121 aagccgaaat cgccgaactg ccggacgacg aagccgaagt cttcctggaa gaactgggcc
      181 tgcccgaatc gggcctggac aagctgatca aagccagcta ttccctgttg ggtctgatca
      241 acttcttcac ggccggatct gacgaagtcc gcgcctggac catcgtcaac ggcacgaaag
      301 cccagaaagc agccggtaag atccacaccg acatcgaacg cggcttcatc cgcgccgaaa
      361 tcgtgtccta cgacgacctc atcgcctgcg gcagcgaaca agctgccaaa gaaaaaggcc
      421 ttgttcgtct ggaagggaaa gattacgtca tgcaagacgg agatgtcaca tacttccgct
      481 ttaatgtata ggaaaaaaag gcttgtcgca tatgcgacaa gcctttttaa gtggttagtg
      541 gctagcagtt aggggttagc ggatgtgttt taaatttaaa aaccatctgc tacaaactat
      601 aaactccgta ctacaaacta gtatcgtcct cccgatgttt tgggcgtggt tcccgttcat
      661 ctgtcaatcc ggcaatgtaa tcgagtgagg tgtggtacag ccttgccagc tggatcaact
      721 tttctacggg gatcttccgt ttccccaatt catacatgcc gtatccaact tgtgaacact
      781 tcaagatttt tgccacgtcg gcctgagaaa gccttgcttc caaccgaagc gtctttaagc
      841 ggctgtagta tttcatgtca tcacctgttt tcatagatct aggactataa taagtacatt
      901 agtaaattcc ttgattctat tatagtaaat atataaaaag acggcaacga ttattatgaa
      961 tataatcgtt gccgtctttg caatttgact tcccctctat gaaataatta gccgatttct
     1021 actaattttc caggattcat gatgttattc gggtccaaag ccttcttgat ggctttaatg
     1081 acgttaagtt ccgcctgcgg tgtgcaaagg gcaaattctt ccaatttctt atagccaatg
     1141 ccgtgttcac cggacatctt gccgcccagg gcatagacgc gcgggaagag gtcgcgatgg
     1201 aaggccttga tcttctggaa ccattcttcc ggcgacaagc ctttggcatt cagaggcagg
     1261 acgtggatgt taccatcgcc gatgtgagca accgtgacgc agtagaggtc gtatttttcg
     1321 cgcagttccg ggatctgttt ggtgatttcc ggaatcttgt ccaaggggac gacgaagtct
     1381 tccgtctgga acatcttgtc aacgtcgcgg gcggcttcgg cgaactgctt gcgcagagtc
     1441 caaatccgtt tatccgcttc aaattcgtcg atcgagccgt tggcttccgc caagtcgcag
     1501 agctgttcca tcttgcggtc cgagtcgtct tcgctgaacg attcgaccgt gacgatgacg
     1561 taatcacagc cttcgtcgac atgaggcatt ttcatgtcca ggaatttgca ggtcatgcgc
     1621 agggcttcat tgtccatgaa ttcgatgctc gtcgggtcga tgccggcttt gaggatctta
     1681 ttgggcaggg caaaggcttc ttcatcggtc ttgaagacgc agaccatatt gaaggcatac
     1741 ggcggcatgg gacggagttt gaccgtgact tccgtaatga tgcccagcgt gccttcggaa
     1801 ccggcaaaga gctgttccag gcagaggccg gtagaactct tcttgaggcg ggcaccggcg
     1861 tcgaggatgt cccctgtcgg cgtaacgact ttcaggctgt agatctgatg gcgcgtcgtg
     1921 ccgtatttaa cggctttatt gccgccggca ttgttggcga cgttgccgcc gatcatgctg
     1981 ctgtcggcac tggacgggtc cccggcatag aggaggccgt gtttggcagc ttcacgctgc
     2041 aagtccccag tgatgacgcc agtctggcag acggcataca tattgtcggc atcgaatttg
     2101 aggatctgat tcatccgttc caattcgacg accatgccgt gataaacagg aatagcacca
     2161 caggcaacac cggaaccagc cgagcgcggc gtaatcggaa cgaggtactt attggccaat
     2221 ttgacgatag cggccacttc ttccgtcgag cccgggaaga cgacgacttc cggtttatgg
     2281 aaataatggg agttcccttc ttcgtctgtc tggtatcttt ccaaataatc ttcgtccgtc
     2341 ttgacgtatt tatcaccgac gacctgacgt aattctgcta ataattccgg cgtaaccggg
     2401 ttaaactgtg ctgccatata taaacgcttc ctttctatcg gaatctttcc gtatgcttta
     2461 aagatataat aagtttatcc tccgaatggg agttgagcat agtataccac gtagcagaaa
     2521 cttgtaaagc ccgttttctt gacattttta aaaaacgcat caagaaaacg agctttttga
     2581 aaccacacat tgggatggat acgtaaagtt ttacgtttta cgctatttcc aatcctaaag
     2641 tttatattta tttttcatat cttccatgca cggacagttt taagactccg aaagggcttc
     2701 ccacttttca taagcctcat cgagctgggc ctgggccact tcgtattcct tcgtcaattc
     2761 catcatggcg tcggcatcgg tctggttctg gggcatattc atctgcactt catacatctt
     2821 gatagtcgct tccagttcgg caatcttcat ctcgattttt tccagcttgg ccgcctggcc
     2881 ataagccggg gccttggccg gcgctttcac ggccggtgcc ggttccgcct tcttcactgc
     2941 cggagctgct gccttttcct gagcctgtga ggagccttgc gcttcagctg ccgccttttc
     3001 tgccgcttcc tgggcttcac ggaccttttc tcggtaataa ctgtaattgc cgacgtattc
     3061 cgtgagctgc ttatgttcca ggaccagcgt ccgctgggta atcttgtcga ggaaataacg
     3121 gtcatgggat acgacgaggt acgtcccgcc gaaatccagc aaagcctgtt ccagcacttc
     3181 cctcgtcggg atgtcgagat ggttcgtcgg ttcgtcgagg ataaggaaat tcggcccctg
     3241 caggaaaagc ttcaacaggg ccagccgggc ctgttcgccg ccgctgaggc tgtcgatgac
     3301 cttgaagaca tcgtcgccgc ggaacatgaa gcgccccagg acggaccggg cttcttcttc
     3361 gccataactg aaatcgttca tgatttcatc gaggaccgtc caggccccgt gcaaatccgt
     3421 gtgttcctgg gcaaaatagc cgatatggac gcggctgccc aaggtaatat ggccgtctgt
     3481 cggttccttt tcgcccatga tcatgcggat gagcgtcgtc ttccccgtcc cattggggcc
     3541 gatgagggaa gccgcttcgc cgcggcgcag gaggagcgat aaatggtcga acaactggcg
     3601 gtcggcaaag ccgccgctga ccttgtccag gaccaggact ttctggccgc actcttcgac
     3661 gggcttgaaa ttgaagtgca gcgactcatc ttcgggctgc cattcgatgc gttccagccg
     3721 gtccagctgg gactggcgcc cgcgggcctg cttggccttg atgccggctt tgtatttgcg
     3781 gatatattct tccgtttcgt ggatgtgttc ctgctgcttc ttataggcct tttccagggc
     3841 cttctgctgc tgggcatgct gctcgacata gcgcgaataa ttgcccttgt atttagttac
     3901 cttgccgtgg gacagttcga ggatgcccgt cgccacgcgg tcgaggaaat aacggtcatg
     3961 ggatacgatg aggatgccgc cgccataaga caagagatag ctttccagcc attccagcat
     4021 gtccacgtcg agatggttcg tcggttcatc gaggaacaaa taatccggcc ggcggacgag
     4081 ggccttggcc agattgatgc gcgtcttctg gccgccagaa aagctctgga ccggccggtc
     4141 catgtcggct tccgaaaagc ccaggccctg gacgattttc cgcgacatcg attcgtattc
     4201 atagccgtcg agccactcga accgttcctg catgcgggca tagcggttca tcaagccttc
     4261 atcatcggga tgggcctgca tggccttttc gacagccttc aaatcctttt ccagcttcat
     4321 gacgtcctgc caggcctcgg taatcgtctg gcgcaaggtc acatcgtcgg cgtaagtgat
     4381 atcctgctgc aaatagccga tggattcgcc ttcggaaacg acaaaggccc cgttgtcgta
     4441 gtcctcttcg cccatgaggc acttgagcag cgtcgacttc ccggccccgt tggccccgat
     4501 gaggccaatc cgttcgcccc ggcggatttc aaaactcaca tcatggaata ccgtatgcac
     4561 tccaaaagac ttactcaatt tctgtacttt aatactaccc atctatacaa tcctgccctt
     4621 cctggcatac tgacgacaaa acaaaaaaag agccgcccgc gactcctatc ttgagcggct
     4681 actcctgtag caattcatga aggtcgaccg tcccgcggca agaccccttt tcacatatct
     4741 attataccgt caaccctcaa tcatcgtcaa gactcggatt ccgatgcggc tttgccgcgt
     4801 caaattcatc ccatttgtca taaattccgg ctatctacga aattcacacg ggacgcatta
     4861 acaggacgtc ccctacggta tcgcatagag gcgatgaaaa taataagcag gccgctgttt
     4921 acaggtggca ggccgcaaaa acactgcctg cggccttgtg cataggggct tccacgtggg
     4981 aagcccgcta gagagatttt agtcctatgt caagtttgag tacaagttaa attgaggttt
     5041 ttatcaatca gttatccagc aatcaaagct gcctttttct tgtagtttgc atataatttc
     5101 tcatagtcat ttggtgacat atagttacag tgactgtgaa ttcgaacggt attataaaac
     5161 gtgttaatgt attcaaacac cagcatatag gcttgttgat aattttcaat cttaaatcga
     5221 ttcagccatt ctcgcttaat caaggcatgg aatgattcta tgcaggcatt atcccatgga
     5281 tagcctttct tcgaatagct gcggcacatc tgtgcggttt ccttgatata ggattgcgaa
     5341 agatattgac aaccgcggtc agtgtgaatt actaccagac ggcctggatt cctggtttgc
     5401 tttgccgttt gaagcatcct aataatgcca gatgcatcca acttccggga taaattccag
     5461 gcaatgattt ttcgagaaaa taaatccatg atactggcca aatacacaaa gccttcagta
     5521 gtccaaatat aagtaatatc gctgcaccag atcgtgtctg gtcttggcgg attaaattgt
     5581 tcttgcagaa tattaactaa tatggcatcc aaacaactgc tctgggtcgt aacggtataa
     5641 tgcttgaccc aacaagcacg aatccccatt tgccgcatgt aaaggcccac tgtcctttcg
     5701 gaaatacatt cacctttctg ttgaagcaat ttagtaattt tgggtgctcc atagatttgc
     5761 tttgaatctt cgtaaagggt ttgaatctgt ttttttacct ccttacggtg cgtagcttgt
     5821 ttggataatt tgcgatgact ccatccatag taacctgatg tagaaacgcc caattctgcc
     5881 agcactccag ataccgaaaa atggcgtttc ccctgatggg ctttttcggc ttcctgtttg
     5941 acgctatggt aaatggcggg gattatttgt ctagaatgtt gatagctttt tttaagacat
     6001 tgatggcatc cttggcatct cgtaattcac gtttcaaacg agcgatttcc ttggcttcat
     6061 cagatgcata gtttccggat ccgcgatatg gcatttcccc attatcccgt aattgctttt
     6121 gccatcggga cagtgtctgt tgggaaattc ctaaattatg ggcacaccca atcagtccta
     6181 agtcacgatg atcatggtaa tattggacag catccatttt aaattgtttg tcgaacttcg
     6241 tcatactaag gccccctata tatactttta ttgtatcact ttatggaaaa cctcagtttg
     6301 acttgtccta tttatatgct agcaccatac taacaaattt aagggacagt caggcaaccc
     6361 caatgaggtg ggatatgcta ggaccatcag ttgccaccct gaaccatgaa atggggaaaa
     6421 tatttgacct ctgtctgcct tctgctattc attgtcaatc ccctgaacgg accttcttcg
     6481 ttggaaacaa aagagatggg gagtgtaa
//
LOCUS       DAWCYS010000073         6411 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_729_length_6411_cov_4.599591, whole genome shotgun sequence.
ACCESSION   DAWCYS010000073 DAWCYS010000000
VERSION     DAWCYS010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_729_length_6411_cov_4.599591"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6012..6021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1251 a         1772 c         1885 g         1493 t
ORIGIN      
        1 gcaggcggac ggcgcggagg acgccttcga aggacgccgc gtcttcagcg atgccgtcga
       61 ggtaacgctg gcgaaggccg tgagccttat cgcggatgga taccatgagt ttctcgattt
      121 catcgtagcc cttcttgccg atggtcaggt tggcgaccat ctctacgagc gctgctgccg
      181 aagcagcggt catagcggcg atgccgccgc cgccgggaac gggttccttg gaagccgtga
      241 cttccaggag cccgtcgagg ggcatggatt ggaattgtgc aaaatccatc ttagaacaat
      301 cccgtaatga tgccattttc gtcgatgtcc atgcggttgg cagccggtac tttcggcagg
      361 cccggcatgg tcatgatatt gctcgtctgg cagacgatga agcccgcacc ggtgcagacg
      421 cggacgtctt tgaccgtgac cttgaagccc ttcggtgccc ccaggagcgc cgggttatcg
      481 gacagggaat actgcgtctt ggccacgcaa atcggcattt tatcgaggcc aaaggcttcc
      541 agttccttga tctgtttctg agcgttgcct tcaaagagga cgccgtcgcc gccgtagact
      601 ttcttgacga tagtttccag tttttcagga atcgaagcgt tgacgtcgta gagcggtttg
      661 tacggggtct tcggctgctg cagcagtttc aggacttttt cgcccatttc gacgccgcct
      721 ttgccgcctt cggcccagca gttgttgagg gcgacttcga cgccgtaatc ttcgcagagt
      781 ttccgcagcg tagcgatttc ttcatctgta tcgctgatga atttattgat gcctacgacg
      841 acggggacgt cgaagaggcg catattttcg atgtgtttgg ccaggttggc aaagcccttc
      901 ttcaaggctt cgacattttc ttcctgcaag ttcttcttgt cgacgccgcc gtgcatcttc
      961 agggcacgga cggtcgcgac gatgacgaca gcatcgggtt tgatgccggc gaaacggcat
     1021 ttgatgtcca tgaatttttc agcacccagg tcggcaccga aaccagcttc cgtaacggcg
     1081 atatcgccca tcttcatagc cagtttggtt gcgacgacag agttgcagcc gtgggcgatg
     1141 ttagcgaagg gaccgccgtg gaccaaggcc ggcgtatgtt cgatggtctg gaccaaattc
     1201 ggcttgatgg catctttcat gaggatggcc atggcgcctt cgacgccgag ctgacgggct
     1261 ttgaccagat tgccgtccag gtcataggcc acggtgatgt cgcccatgcg cttcttgagg
     1321 tccatgaggt cgctggccag gcagagggct gccatgattt ccgaagcgac agtgatcatg
     1381 aaatggtctt cccgcgggaa accgcagact ttgccgccca tgccgacgac gacgttgcgc
     1441 aaagcccggt cgttcatatc catgacgcgt ttccagatga tgcggcgcac atcgatgcgc
     1501 agggcgttgc cctggtggac gtggttgtcg atggctgcgg ccaagaggtt attggctgcc
     1561 gtaatggcgt gcatgtcacc ggtgaagtgc aggttgatgt cttccatggg caggacctgg
     1621 gaatagccgc cgccagcagc accgcctttg atgccgaaga ccggccccag ggacggttcg
     1681 cgcagggcga cgacagcctt atggcccatg tagttcagtg cctgggccag gccgatagtg
     1741 gtcaaggtct taccttcgcc agccggtgtc ggcgtgatgg ccgtgaccag gaccagtttc
     1801 ccgtcttcat tcttcgacag gcgcttgatg gcgtcaaaag aaatctttgc tttatatttt
     1861 ccgtagagtt ccaagtcatc atcggtgagg ccgacttgtt ttgcaatggc cgtaatcggt
     1921 tccaatttcg tctgctgtgc aatttcgata tcgctcaata cctttgccat agtagttgtc
     1981 actcctgttc ttttctttgc ataaaaaaag ccgctccgct ggaaggttgc ccagacggtc
     2041 ggcttcaaga aatcatgctc cccgtggtaa ctccacttcc gtcagtcaca tgattatggc
     2101 ttcattatac aaaaaaagaa ccctctggtc aagggttctt tttcaggaca tcttactgga
     2161 tcacgctggt atcgaggtgt tcaaagcccg tgagcaggcc tttcacctga ttggcgatct
     2221 gctgacagcc gcctttttcc ctgctttcga tgttcagcgg catgttcggg tcgtggaggg
     2281 acatgcgcag cagggcccag ccgttaggga atacgagacg cacgccttca taggacggct
     2341 tggcgatggc gatgcccgct tgagcggccc gttcttcaaa ctgtttaagt acgtcagcgc
     2401 cgtaggcctg gacgtcgtct gtgccggtga ttttcaggcg gaattcgacg ccttccgcag
     2461 gatggcccaa tttggcaatg aggtcgccga gctgtttgcc ttccttcttg gctttcgtcg
     2521 cagcgatgag cagtttgacg gccaggtacg cgccgtcgtc gaggtagtag ttttcactca
     2581 gcgcgccgtg cccggatgtt tcgatggcca gcggcgaaac ggtgccggcc ttgttgaggc
     2641 ggatacattc gtcgatgaca ttcttatagc cccgcatata gcggtgatgg accagatgga
     2701 gttcatcttg gaggaacgtc gtcaattcgt cggatgtgac cgagtcggtg acgatagtgc
     2761 tgccgggata atcaggcgcc aagatggcgg ccatcatggc gatgagggcg ttgcggttga
     2821 tttcttcccc atcgggcagg acggcgctca tgcggtccac gtcggtgtcg aaaatgaggc
     2881 ccaagtcggc tttgttgtcg aggacggcct tgcggataga ggccatggca tcggcatttt
     2941 ccgggttggg gatatggttg gggaacatgc catccggttc gaggaactgg ctgcccgtcg
     3001 tatcggcgcc gagttccgcc aggaccttag tggcaaagaa accgccgctg ccgttgccgg
     3061 cgtcgatgac gatgtgcatc ccttccagag gacggtcgta catgccggaa cagaggccgg
     3121 cacagatctt acggcgcagg tgcagggcgt agaggtcgat cagatccagc gaggcaatgg
     3181 ctgccgatga tacggccatg gccgacaggc ccgaagccag ggtcaggact tctttgactt
     3241 cgctcttttc caggccgccg tcgacggtaa agaatttcag gccattgcgg ttatagggca
     3301 aatggctggc cgtaatcatg atggagccat ccatgtgggt atcttcaaaa ataatggaca
     3361 tgaacatggc cggcgtcgaa gccaggccgc agtccgtgac gtggacgccc tgaccgagga
     3421 ggccgccgaa tacggcttct ttcatcatgc ccgccgacac gcgggaatcg tgtccgacgg
     3481 caatgcgcag gtcactcgtc tgtttattca gcttcttggc taaaaacttg gcaaatccag
     3541 cggcaatgcg gttgaccgct tccgggacca aggtgacgtc ttcaccggga acgccggcga
     3601 tggcgacgcc gcggacgtca ctgccattct gtaatttcat catcgcttct gtagttatca
     3661 tgtagggatg tttcctttct tcgatttagc ggacagcgtc cgctgcctct tatcatactg
     3721 aaaatcaggc caaaaggcaa gcgtcgacaa tggcttctgc cgtcaggccg tactctttca
     3781 agaggtctcc ggccttgccg gattcaccga aacaatcctg gatgccgacg taagcctggc
     3841 gcgtcggaca gcggcgcgac aaggtttctg ccacggcgcc gcccaggccg ccgatgacgc
     3901 tgtgttcttc ggcagtgacg acgaagcccg tcttcctggc tgcagcgacg accaggtctt
     3961 cgtcgagcgg tttgatggcg gccatgtcga tgaccatgac ctgacagccc cgattggcca
     4021 ggatatcggc tgctttcagg gcttcctgga ccatgaggcc cgtagcgatg acggcagcat
     4081 cacagccgtc gcgcaggact gtccccttcc cccactggaa gggacagcca gattgataga
     4141 cgtcgtcgac tttggcccgg cccatgcgca cgtagaccgg cccgtcatga gcgacagccg
     4201 cccggatgac agcttccgtt tcggcgccgt cagccggaca gacgacggtc atattcggca
     4261 gactgcgcat gatggccatg tcttcaacgg actgatgggt cgccccgtct tcgccgacac
     4321 tgaggccggc gtgggtcgca cagatcttga cgttgagctt tggcaggcag atggagttgc
     4381 gaatctgttc aaaggcccga cccgtgccga agacggcaaa agtgctggcc aggggaatct
     4441 tgccgcaagc ggcgaagccc gccgccgtgc ccatcatatc ctgttccgaa atgcccatat
     4501 cgaaatagcg ttccggcgct acctttttaa aagcaatcga ccgcgtcgac gaaccgaggt
     4561 cggcgtcgag gacgacaatc ctcggatttt tatacaattc cgtttctaag agatgcccgt
     4621 aggcatcgcg tgttgccctt gccatcagcc ttcgccccct aattcacgta acgcccgttc
     4681 acattcttct ttcgtcggcg ccttgccatg ccaggacacg gcattttcca tgaacgatac
     4741 gcctttgccc ttgacggtct gggcgatgat gaccgtcggc ttcccttttg cggcctgcgc
     4801 ctggtgcagt gcagcgccga tggcgtcgta atcgtggccg tcgatttcca ggacgttcca
     4861 gcggaaggct cggaatttgt cggcaatggg catgacgtcc atgacgtctt cattggaccc
     4921 gtcgatctgc agtccgttat ggtcgatgaa cagagtcagg ttgtccagtt tatactgagc
     4981 tgccgacatg gccgcttccc aaatctggcc ttcttcgatt tcgccgtcgc cggcgatgac
     5041 gtagacccgc cagtccgccc cgtccagctt ggccgtcatg gccatgccgt tggcagctga
     5101 caagccctgt cccagggatc ctgtcgtcat gtcgataccc ggcgtcgtat tcatgtcagg
     5161 attgccctgg agcatggcgc cgcactgacg caggtgccac aattcatctt ccgggaaaaa
     5221 gccccgttcg gccagcgtcg catacagagc cggtacggta tggcccttgg agaggacgaa
     5281 acggtcccgg tccggccagt gaggccgctt gggatcgatc cgcatgacat cgaaatacaa
     5341 gacttgcagc atatcgacga tggacaaaga gccaccggga tgaccggact gggccagggt
     5401 gaccatcttg acgatatcct gccgcacgtg agccgcatga cggctcaatt ctttgctatc
     5461 catgagaaag cccccttttg ccttccatta tagcacaaaa gaggctgccc ggcggcagcc
     5521 tcttttgtga gtttgaagtt tgcagtttgt agtggatggt cataaacttg aaaccatctt
     5581 ctacaaactc cgtactacaa actataaaga gaaatgggcc tgcatgatgg caaaaagctt
     5641 cgtgcaacag tctcttttaa gtggctagtg gttagtggat ggctttagat gtaaatgccc
     5701 attggctctc ttcataggag agctgtcagc acagctgact gagaggttca acctctccgg
     5761 ccctgcgggc cacctctcct atcaggagag gcttacggtc tgcgacctgc ggccgcggcc
     5821 ttcgcagttc agtttcttac gagccacgag ccacgctcac aacacatact tctcgatggc
     5881 ttcggcgacg gcgccttcgt tattggtggc gtcggtgacg acgtcgcagt attggcggat
     5941 gacggggttg aggttatgga cgccgacgga aaggccggcg gccgtcagca ggtcgatgtg
     6001 gttgaagttg tnnnnnnnnn ntgtcgccat tgtccatggt ttcatcatgg gctacacgga
     6061 ggatatccgc caatttccag aggcccgccc ctttattgac gcctttgtgc atgaattcga
     6121 tatagcggtt gctggagaag ctgaccgtaa tgtcgtccag caaggggcgg atttcagcgt
     6181 gtatctgctg taaatagtcg taatccgtat tggtcacgat gagcttgcag acttcttctt
     6241 tgccgcggat gaaatcgagg tcctccgctt cgacaggcac gtagcccatg cggccgtgga
     6301 ggaaggcttc ttcgtcgggc gtaatattcg tgatgtagac gacatcacgc gtatagatat
     6361 gcatacattg accataggta cagcagcggc ggaaaatccg gtcggccagg t
//
LOCUS       DAWCYS010000074         6378 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_731_length_6378_cov_5.970425, whole genome shotgun sequence.
ACCESSION   DAWCYS010000074 DAWCYS010000000
VERSION     DAWCYS010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_6378_cov_5.970425"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1538 a         1885 c         1691 g         1264 t
ORIGIN      
        1 catcagcagc tgaaggccct gtaatcctat tcgtcgtaca tggtcctgac cttacatatc
       61 acgtaattta ttcttgattt cgtgtgctat gattctttgc tgggcaaacc cgtcggcagc
      121 gacgcccgca acgacaaggc cacgtatgtg accatctttt ccctggacga agcccggcgc
      181 atggcccgcg aagcggccgg caaggccgtc caggccctgg aaccgttagg gccgcaggcc
      241 tggttcctga aagaactggt cgaacatctg ctggtccgcg ttcattaatg tgagaggggt
      301 attacaggta tgcagcataa agaaagatta gacgtgcttc tcgtcagccg cggcttgtct
      361 gaaagccggg aaaaggccaa ggcgaccatc atggccggcc tggtcttcgt cgacaaccag
      421 cgcgtcgata aggcgggcac gaagctgccc gtcgacgtag atattaccgt caagggcaat
      481 cccattccct atgtcggccg gggcggcctg aaactggaaa aggccatgga aacgtttccc
      541 atcgacttga cgggtaagac catgatggat attggcgcct cgacaggcgg cttcacggac
      601 tgcgccctcc agcgcggcgc ggccaaggtc tttgccgtcg acgtcggcta tggccagctg
      661 gcctggaaac tgcggactga tgaccgcgtc gtcaacatgg aacggaccaa tatccgcaac
      721 gtcactgttg cagatatcgg cgagcccgtc gatttcatct ccattgacgt ctccttcatt
      781 tccctgctca agatcatgcc ggccgtcgaa ccgctgctca aggcaggcgg cacgatggtc
      841 acgctcatca agccgcagtt cgaagcgggc cgggaacacg tcggcaaagg cggcatcgtc
      901 cgcgacatcc aggtccaccg cgacgtcatc cgccacgtca cggacggcat cgccgactgc
      961 ggcctgtcgc cgctgggcct gaccttttcg cccatcaaag gggctgacgg caatattgaa
     1021 tatctcttgt acagccgtaa agatgacccg tgccataacg atatcaccga cgctgtcgtc
     1081 gatgatatcg tcggtaaatc ccatgaaatg taggtgatcc ccatgcgtat cggtattttc
     1141 ccgaacctcg tcaaacgtga aagtgctgcc attgtccagc agatgatcaa gatttgtgaa
     1201 aaacatggca ttgaatatta cctgccagcc tacgtcagcg agagccgaca gccggcgtat
     1261 cagcgcatcg gcgctgaaca cctgcggccg cgcatgacca tttacagcac catcgacgtg
     1321 gccatgatcc tcggcggcga cgggacgatc ctcaagatgg ccaagcagtt cgctgaagcc
     1381 gatgtcccgg tctgcggcat caacctgggc tccctgggct tcctctacga agtcgagacc
     1441 aagaacctgg aaaagcgcat ggaagacatc ctggccggcc gctatttcct ggaagaacgg
     1501 atgatgctcc attcggaact gtgctatgaa gacggcctgg tccagtccct gcccgatgcc
     1561 ctcaacgaca tcgtcatcgg ccacggcaac gtgggcaagc tcatccgcat cgacttgagc
     1621 atcaacggcc acttcatcca gcagtatccc ggcgacggcc tcatcgtggc tacggcgacg
     1681 ggtagtacgg gctatacttt ctccagcggc ggccctatcg tcgcgcccag cgtgccctgc
     1741 atcatggtta cgcctatctg cccgcatctg ctgctgaaag tgcccctggt cctgcgcgat
     1801 gacgaccagg tgtcggtgac cgtggccaac agccgcaaca gcgtccgcgt ctctgtcgac
     1861 ggcatgatgg accaggaact catgcgcaac atgaccctcc aggtccgcaa gtccgaccac
     1921 gtcttgaaga tcatccgctt caacaagaat tatttctata gcaacttatt tacgaaaatg
     1981 atgggaaatg attgacatgt atccattccg caatgccgtt acggtatcac atatgatgac
     2041 tgaaccctac gcccgccagg cccatgtcct cgtcgacatg acctgcggca acggccacga
     2101 cacggccttc ctggcccgca ccatgcctga tgacgccgtc ctctacgcct tcgatatcca
     2161 gccctgtgcc atcgagcgca cgcgccagcg cctgcaggac gaaggcctgg gggacaagga
     2221 cgtccgctgc cgctgcgggt cccatgaaca actgctggct gatgtacagt cagacattga
     2281 cattatcgta tttaatttag gctatttgcc atctggtgac cataaaatcc atacaaattg
     2341 tgaaataaca ctaaaagcta taaaaatagg cttgaataaa attgcgataa atgggataat
     2401 tatgataaca gcctatcccg gtacggaggc cggggcttgt gaagaacagg cgctgcggtc
     2461 gttcttacag accatacccc agcaggactt ccatatttcg tcctggcaac ccgtcaatga
     2521 ggtgcattgt ccacccgtat tatatattgt acagaaaaga gggaaacgtc attatgaaga
     2581 gattccgtta caccaagatt aaagaaattg tccagtcccg tcctatcgaa acgcaggaag
     2641 aattagccaa ggccttgcag gaagaaggca tcgaagtcac acaggctacg gtttcccgcg
     2701 atatcaaaga gctcatgctc atcaaggtcc cgaccagtga tggccattac cgctacgccc
     2761 tgtcgccgga acagaacatg ctcatgtcca agaaccgcat ggcccgcctg ttccaggact
     2821 ccatcgtccg cgtcgattcg gcgtataacc agatcatcgt ccataccctg ccgggttcgg
     2881 ccaatcccat tgccgccgcc atcgaccatg cccgctggga aaatgtcatc ggcaccctgg
     2941 ccggcgacga cacggtcctc ctcatcgtca aggacacgga aacggttccg aaactggtca
     3001 aacttatcgt atcgctgatg aaggaatgag gcccttatgc tgcagtccct gcacatccac
     3061 aattttgctc tgattgaaga cctgcacctt acttttggcg acggggtgac gatttttacc
     3121 ggcgaaaccg gcgccggcaa atcgattctc ctcgacgcca tcggtatgct ggcaggcaaa
     3181 cgcgcatcgg catcgttcgt ccgtcacggg acggactcat tccttgtcga aggcgcgttc
     3241 tttttttctc ccctgccaga agggctggaa cagctgctga ccgataatca catcgaagtc
     3301 gatgaaggcc agctcatcat ttcccgccag ttccagcgca gcggccgcgg gacgatcctg
     3361 gtcaacggca cgctggtgcc gacgacagtc ctgcggcgcc tcggcgcata cctcctggat
     3421 atccacggcc agttcgacaa cagcctgatt ttcaactcgt cttaccacgt ggccatcctc
     3481 gacggcctca cggccgacgt ccgtcaggcc cgccaggact atgacgaact atatcgccag
     3541 tggcatcaga cggaaggcga aatcaaggcc ctgcgccacg atgaaagcga aaaggtgcgg
     3601 aggctcagta tcctggcctt ccagatcaag gaaatcgaag gggccgccct gaaagaaggg
     3661 gaagacgacg aactggaaca gaaaatcaac aaggcttccc atgccgaaca catcaaggat
     3721 aacctgaaag aagccatgtt tgccctggaa gggggcgaac gccagcccgg cgtcatcgaa
     3781 cagatcgaga ccatccaccg cagcctggaa aaggcgtcgg cttacgatga agctttcagt
     3841 gccctggccg gcaaagtcga gactctgtcc tatgaaatcg aagacgtcca cgacggcctc
     3901 ctgagctatg ccgattcttt cgacttcgat gaacgggccc tggatgccat gcagtcccgc
     3961 ctggcgtcga ttgaaaagct taagcgcaaa tacggcttca ccgtcgccga tattctgcaa
     4021 ttcctggcca aggccaaggc cgattacgaa cggctcgacc agtccgaaag ccacctggcc
     4081 aagctggaag agaaattggc ccgtcaggcc gcggctgtgc gccagcaggc ccaggtcctg
     4141 gcggccgcgc gccgtgacgc cgacagccag ttcagccaga ccatgacggc gacgctcaac
     4201 aagctgggca tgctgaacag ccgtatcgcc ttccacatcg aaccgatgaa ggacatcaca
     4261 gcggatggtt cagccggtat cgagctctat ttctcggcca atacgggcga agatatgcag
     4321 cccctggcca agatagcatc gggcggcgaa gtatcgcgta ttgccctggc cctgaagacg
     4381 gccggtccgg aacggacgac gggcaagacg atgatcttcg acgaaatcga cgtcggcatc
     4441 agcggccaga cgggcctgca ggtcgccgac cacatccgcc agctctccgg ccagggccag
     4501 gtcttctgca ttacccacct gccgcagacg gcggccattg ccgaccacca ttatttcata
     4561 tataagaaag aaatggacgg ccgcacggtc acccaggtcc ggggcctctc ggaaggggaa
     4621 cacgtcctgg aaatcgcccg catgttcgcc ggcaacgata cgaccaaggc cagcatcgaa
     4681 gccgcccgcc agatcgtaaa acaagtcaag ggcatgtaat gcccttgact tgcgattcga
     4741 cattgcttta cgggttgacg aatgtccgtg ataggtatat agttaattat aatttcaaaa
     4801 aatagaagtt ctacgaaaac gaacccatag gagggataga tatgcttcgt gtattagtca
     4861 atggtgccga tggccgcatg ggcagccagg tcgtcaaagc tgtctttgaa gatccggaat
     4921 tggaagtcgc tggcggtatc agcatcactc atgtcggtga agatgtcggc gatatcgccg
     4981 gtgtcggcca cttaggcctg gccatccgca atgacctggg cgccgccctg gacgacatca
     5041 aaccggatgt cgtcgtcgat ttcacgacgc cgaaagtcat cttcaacaac gccaagacct
     5101 gcatcgaaca cggtatcaac atggtcgtcg gtacgacggg cctgacggca gaccagcgcg
     5161 atgaactcaa cgccctggca ctggaaaaga agaccagcat cttcattgcg ccgaactttt
     5221 ccatcggtgc cgtcctgatg atgaagctct cgacagaagt cgctccgtat ctgccggatg
     5281 tcgaaatcat cgaactccat cacaacaata aactggatgc gccgtctggt acggccatca
     5341 tgacagctga taaaatcgaa gctgcccgca aggccgctgg tgctaaaccg gctccggaca
     5401 agacccatga aagcctcgac ggcgcccgcg gtgcctctgt cgccgggatc cctatccaca
     5461 gcgtccgcct gccggggtac gtcgcacatc agcaagtctt atttgggggg tacggcgaac
     5521 tgctgacaat ccgccacgac tcgatggacc gcaagtcctt catgcccggc gtcgtcttag
     5581 ccgtcaaaaa agtaagctcc cgtccgggct tgacctttgg actggaaaac tatttataaa
     5641 agaaccatta ggggaggaag tttgttatga caaaagaacc ggtagtagcg attttaggtg
     5701 ccacaggtgc cgtaggccag gaatttatcc gcttgatcgg agaacgcaaa ttcccgtaca
     5761 gcaagttgaa gttattagca tcgtaccgtt cggctggcaa gaaattgacc gtcaacggcc
     5821 aggaatatac ggttgaagaa gccaaaccag aatctttcga cggtgttgat atcggcttgt
     5881 tcgccggcgg ctcgatcagt aaggaattgg gacccgaagc tgctaaacgg ggctgcgtcg
     5941 tcatcgacaa ctccagcacg ttccgtatgg acccggatgt ccccctggtc gtaccggaag
     6001 tcaaccctga agatattgcc tggcacaagg gcatcattgc caacccgaac tgctcgacga
     6061 tcatcatgct catggcactc aagccgattc acgacttgtc gccgatcaaa cgcatcatcg
     6121 tcagcaccta tcaggctgta tccggtgccg gcaaagaagg tatcgacgaa ctgcgcgatg
     6181 aaacggaagc gtacctcaaa ggcgaagaaa tgcagcccaa gatcctgccc agcaagagcc
     6241 tgcccaagca ttatcccatc gccttgaacc tcattccgca gatcgataaa ttcgtagaaa
     6301 aagattagac caaagaagaa atgaagatgt tcaacgactc gcacaagatg ctccccgatg
     6361 actccatcgc cattccgg
//
LOCUS       DAWCYS010000075         6339 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_737_length_6339_cov_4.991725, whole genome shotgun sequence.
ACCESSION   DAWCYS010000075 DAWCYS010000000
VERSION     DAWCYS010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_737_length_6339_cov_4.991725"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1219 a         1709 c         1805 g         1606 t
ORIGIN      
        1 cggcgggctc gtcacgtgcg agcctctact tattttatga cgaagttgat gattttcttc
       61 ggtacgacta tgaatttgac tactttctta tcgccgatga attcctggat gtgcggtaaa
      121 gccaggactt tttcctgcaa ggcatctttg tcgatgtcga cggctacggt caggcggtcg
      181 cgtactttgc cgttgatctg gacaggcagt tcgatttcat cgacgacgag agccgatttg
      241 tcgacttccg gccaatcctg ggcatggacg ctgccttcaa agccgcattt ctgccagagt
      301 tcttccgtaa tatgcggtgt gaagggagcc aggagcttga gcaggttctt gttggcttcc
      361 agggcaacgg ccttgtggat ggtcttgtta gcgccgacat agccatgcat ggcgttaaca
      421 tattccatga tcgagctgac agccgtattg aggccgtagt tgccgtctgt gccgcggacg
      481 tcttctgtga ccttggcaat ggctttgaat tcctggaaac gcagttcttt ttcggcttcc
      541 gtatagtccg tcccttcaag gccgtctttg accaagtcct ggaactgatg gacaatgcgc
      601 catacgcggt tgaggaagcg gtaagagcct tcgacgccct ggtcagacca ggccaggtcg
      661 cgttcgggcg gagccgcgaa gaggatgaac aggcgggccg tatcggcgcc gtatttggcg
      721 atgatttctt ccggcgatac gatgttgccc ttggacttac tcatcttgct gccgtcgagg
      781 aggaccatgc cctgtgtcag caggcgttcg aaaggttcga cattgttgac caggccggcg
      841 tcatagaaga ccttcatgaa gaaacgggaa tacatgaggt gcaggatggc gtgttcgatg
      901 ccgccgatat actggtcgac gttcatccaa tggttggctt tcttcggatc ccaggctttt
      961 tcatcattgc gggcatcggt atagcggaga tagtaccacg acgagcagac gaaggtatcg
     1021 atggtatccg tttcacgcgt agccgggccg ccgcatttcg ggcacgtgca gtggacgaag
     1081 tgttcgctcg tagccagcgg cgatacggcg ccagccgtga acttgacgtc ttccggcagc
     1141 ttgaccggca gctggtcttc cggtacaagc tgttcgccgc atttcgggca gtagatgata
     1201 gggataggag cgccccaata gcgctgacgg gaaacgagcc agtcgcgcag gcgatagttg
     1261 acgcggcgtt tccccagtcc tttttcttcc atgtagtcga tgatggcttt catggcttta
     1321 tgcatttcca tgcccgtgaa aggaccggca ttgaccagga cgccgtcctt gtcttcatag
     1381 gcatggtcca tttcttccag cttcagttcg tggtctttcg gctgcaaagt gatgatcttc
     1441 gggatgttgt atttcgtagc gaacatccag tcacgctggt cacccgtcgg tacgcccatg
     1501 acggcgccgg taccataatc gtagaggacg tagttggtga tccaaatctg gacgctcttg
     1561 cccgtcagcg gatggacgca gtcgatgccg gtatacatgc cgactttttc cgattccgtg
     1621 ctcgtccgtt cgatatcgct ggtattgcgt actttttcgc agaatgcttc cagttcggct
     1681 ttcttgggat tgttttccaa aatcttgtgg accagcggat gttccggcgg caggacgacg
     1741 aaagtgacac cgtaaatcgt gtcaggacgc gtcgtgtaga cggcgacttt ttccttcagg
     1801 ctgggaatgt cgaagctgaa ttccaagcct tcgctgcggc cgatccagtt cttctgcatg
     1861 gtcttgacgc gttccggcca gcccggcagg agtttcagat cgtcgagcag gcggtctgcg
     1921 tagtcggtga ttttgaagaa ccactgtttc aagtcctttt tgacgaccgg tgtgtcgcag
     1981 cgccagcatt tgccttcgat gacctgttcg ttggccaata cggtgttgca tttttcgcac
     2041 cagttgactt tagcttcttt gcggtaagcc aggccgcgtt tgtagagcag ttcaaagatc
     2101 cactgcgtcc atttatagta tttttcgtgg cacgtagcga cttcgcggtc ccagtcatac
     2161 gacaggccca gggctttctg ctggcgtgtc atgttttcaa tattgctgta tgtccattct
     2221 gcaggcggga tattgtgttt gatagctgcg ttttctgcag gcataccgaa agcatcatag
     2281 cccataggat gcaagacgtt atagccgctc atgcggcgat aacgggcaat gacgtcacca
     2341 atcgtgtaat tgcggacatg acccatgtgc aggttacccg acggatacgg gaacatttcc
     2401 aatacgtagt acggcggctt gccgttgtct tcggtgcagc agtcgcattt actttcttcc
     2461 catacgtgct gccatttctt ttctatttcc tgtggaatat acttttccaa ttgtgagagc
     2521 ctcctatcct gccccagtca aaatacagac agactgtact acagcgtctt gagtattatt
     2581 atagtggcag ggcgtcgtca agtcaattca tgttttgcaa aacgctgcca aaaatgtgcc
     2641 ggttcaaagc ctttgcgcca gaaggcagcg ccgcccaaac cataggtctg gaccaggccg
     2701 gctttggctt tcagtgactt ttcatcttca aaccagatct tgacggtccc cgtcgtcagc
     2761 ggcagttcca gataatagag ctgcagatca tcgttccaga cgacatagtc cttataggcc
     2821 ttgaggatgt cttcgctgtc ggccatggtc aacgtgcgga cgaagagctt gcccttgccg
     2881 ccgctcttgc gcgccggagc cgtggctgca gctttgggtg ccgtcctgat acgacgcggc
     2941 aggacacggc gcttatacgt ttccccatcg agttcgacag gcatgatgac cggctgtttg
     3001 gcccgacgga cattttcctg ttccgccgtt acgacctgga ccagcatcgg cccagccgct
     3061 tcttcagctg cttcttctct ggcctcgctg tatttcacgc cctttgccgg cggtgcgccg
     3121 acgacagccg gccagtcgtg gatgtctttc gtcaaatcgc ggccatcgct ggattcatac
     3181 cacaagcgca tgtacaacgg catgcccagg aggactttcg acggcggtac gagatcgatc
     3241 gtattctgta cagcccgttc gacccaggga taggaagcga cagggccggc tgtcgtgctg
     3301 gcccggccga actggtcata ggccatgacc atgaggtagt cgagatgcgg agccagggcc
     3361 gcccggtcat agaccaggga ccagttgggg ctgtccgaga cgggcgtgac atccatggaa
     3421 agctggatac cataggcatg gcaggcctgg gacagtttgg ccacaaaaac cgtcagagca
     3481 tctttatcgc tgtaattgac gttttcaaag tcgatgttat agcctttgaa atcatataat
     3541 aaggcatagc cgacgagctg gcggaccatc gtgtcctgga gacggctgtt gctcaggacc
     3601 tgatgggtca gtttggggtt gaaattgttg tcgatcaaag gccacataac gtatcccttg
     3661 ctggtatacg tcgaagccag catatcgaag tcgggattgc gcagttcgac gccgctttcc
     3721 gtcaggcgga aagcacaggg cgacatgacg ggctgacggg tctgcaaaga cggcaggctg
     3781 gcgtcttcct tcatgacagg gtcccaaaag aggacggtcc ccatcttccc ttccttagcc
     3841 gctgccggta ctgcgtcctg tacctggcgg agttccggta ccgtatggac gggctgggat
     3901 ggcggcagga gttcgttctt ttctgtcgtc ccgttgccca ggacgtagct gatgcccagg
     3961 ggacggcgga catggcggat attgatggtc cggccgccgc tgactgctcg tcctggcaga
     4021 gtcagctgga cgttttcgtt ctgccaggcc gtcgagccgt tctgggcctt cggcaggcgg
     4081 aaccagtagg tccccttggc gtggtcggct tcacggatgc cgtattgttc cagcgccttg
     4141 tcagagacga gccagcgccc ctggtcttcg acgatttcca ggtctttgat ggcttcatca
     4201 gggataggat tggctacggt agatacactg aaggccgggt ccgacgcctt ggcgctgtcc
     4261 gcagccggcc cattggccca ggaaacagca ctcagggaca gacagatcaa ggcggccaga
     4321 caggacgtcc gcataacatg gttgtatttc atcgtcattt gctcctcacg gggattggaa
     4381 aaaagtcctc aatcctgtat aattctatgg taaccgtgaa ccttttcatg ttccgttcac
     4441 attgtttcat tataccacaa caaaggggtg ttttatattg catcaatatc tattttttat
     4501 cggttccttc ccgatccggg cctatgggct gttattcatg ctgggtatcg tttctgccgg
     4561 cattacggct tactatatca tgaaacgcga cggccggggc tggcacgtcc acatctttga
     4621 cttcaccatg tactgcggcc tgtcgggcat cgtcggcggc cgtctatggg acgtcttctt
     4681 cttcgactgg gcctattatc acaaccatct gacggaaatc ccctacgtct ggcagggcgg
     4741 catggctatc cagggcggcc tgctcttcgg cgccgtagcc ggcatcctgt acacgaagta
     4801 ccatcacatc gacacctggg cctttgccga cctcctggca ccgtccatca tcctgggcca
     4861 atccatcggg cgcatggcca acctcatgaa cggcgacgcc ttcggccatc ccacaggcgg
     4921 tgacttcggc atcgtctatc ccgacacgac cctggcctat cacacgtatg gcaatcagcc
     4981 cttgtggccg gccgaagtct gggaaggtca gatcgacgtc ctggtcttcg tcgcgctgct
     5041 cctctacagc tgcttccccc acaagaaggg ccaggtattt tgcctgtacg tcatgctcta
     5101 cgccgtcgaa cgcttcttcc tggaattcct ccgtggcgac tacgtcaacc tgacgctggg
     5161 cctgaaatcg gcccagatga ccagcatcat cgccttcgcc atcgccctca tcgccttcat
     5221 cctcatccag tggaaaggca gcgtggatga aaaagtcagt ggctagtggt tcgtggctcg
     5281 cagcctgcag gccgcaagca ccgcatgggc cgtcctaaaa gcctttccag cccgtcaggg
     5341 gccgacccac ggaaggcgca ggacggttgc tggaacggac agcagaggct ggcggcccgc
     5401 ctgaatctct gtacacgaaa aagaggctgt tgcattaagc aaaaacatgt gcttaatgca
     5461 acagcctctt ttgatttatg ggattcccac aagcgggacg ccctctgggc gtcccctacg
     5521 agccgctagc agctaactgc tagcggctac ttttcttccc gttccagggc atctttcagg
     5581 gtatgccatg ccaggacggc gcatttgacg cgggccggca tgttggagat attcttcagg
     5641 gcgatggcgt cttccagttc ttccaactgg tcatcgtcgg tgacttcccg tttgatcata
     5701 tccaggaaga gggcgaccag gcgcagggct tctttgacgg tcttgccttt gatgaggtcg
     5761 atcatgatgt ccgctgaagc ctggctgatg gcacagccgt ggcccgtata ggcagcgtct
     5821 ttgatgacgc cgtcctggat gtcggcctgg agggtgatgt cgtcaccgca gctcgggttg
     5881 tggccgtgtt cgctgaggtt ggcatcttcc aggggatgtt tattttcctg acactgattg
     5941 tgttccaaga tgatgtcgga atacaattcg ttcatatttt ccatgacttt ctatctcctt
     6001 aatctttaaa gcccatctgt ttgcggacga ggggcagttt ttccaggaag tagtcgactt
     6061 cttccagggt attgtagata tagaagctgg cgcggttcga ggcttctacg cccaagtgag
     6121 cgcctaaagg ctgtgcgcag tggtggcccg aacggatggc gatgccgaag ctgtccagaa
     6181 tggtcgctgc gtcatgggga tggacgccgt cgatggtgaa ggtaatgacg ccggtttttt
     6241 cagccgggtc ggtactgccg atgatgtgga tatgagggat tttcttcatc gcttccaggc
     6301 agtggcgggt gagttcttct tcatgggctt cgatgttgt
//
LOCUS       DAWCYS010000076         6322 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_739_length_6322_cov_7.094942, whole genome shotgun sequence.
ACCESSION   DAWCYS010000076 DAWCYS010000000
VERSION     DAWCYS010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_6322_cov_7.094942"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1282 a         1808 c         1852 g         1380 t
ORIGIN      
        1 aggccgcagg cctgcagcgg gacggacgag tttatgaaat ccctttgaaa agaaaggcag
       61 ggaacatcat gaaggagtgg aagaaatggc tgactgccgg tatctgttgt ctttgtccct
      121 ggacggccgg ggctgtcgat gtccaggtcc gggatgtgcc gacacggacg gtactggaag
      181 ggctggcccg gtcaggaaat atcaatcttg tcgtcgatga cagcgtgcag ggctcgttga
      241 cgatgaacct ccacgacgtc accttggaag aagccttgca ggccattgct gacagccagg
      301 gcttgtatta cgaacaatcc ggccctatcc gtacgatgag cggggcgaaa tcgggaaaag
      361 gggtcaagac ttttcataca tggtcgctac gccatgccga cccggccgtt ttgaaggagg
      421 ccgtccaggc agcggtaccg gaagccgatg tgcgctgcca tggcgatacg aataccctcg
      481 ttgtcggcgg aacgcatcaa gtccaggcgg ctgtccagtc cctggtgaag cgtctcgatg
      541 tgccggcacg gcaagtcgat gtttccgtag aggttgcctc cgtcgatgac agcttgctga
      601 agcagaccgg tgtcgagtgg aactggtcga ctgtagaagg cggggcggac cacggcgtct
      661 tttccctggc cgggacgatc cacgccctcg aagaaaaggg acgggcccat attttggctt
      721 cgccgcacat ggtcgctact aatggccggg aagcgcatat cctcatcggc gataaagtcc
      781 ccgtccagac agagcatctg tccggcaagg aaacgacggt gagtacgacg tacgaagagg
      841 cgggcattaa attgacctac acgccgcaga tccatcccga tgattcggtg acggcccagt
      901 tggtggcaga agtgagtacg cctgtgtttg tgccggaaat gaaggcctac cgcattgcga
      961 cgcgccaggc ccggacggtc gtgcgtctgc gcgatggcga gcctttggtc atcggtggtc
     1021 tgattcgccg ggaagaggtc gagcatttcc gcaaggtgcc gattctggcg tcattgccgg
     1081 tgttggggaa attgtttcgc tatcactata agtcgcataa aaacacagag attgtgttga
     1141 ttctcaggtc gcacgtttta cctaattcgt agtacgtttt acgtgatttt ctttcgattt
     1201 tttcgtcaga ttttgcttga ctttatttta ttttcctgta taatcatggt caacaacaga
     1261 tttcctgata acagttgtga tggaaaaaag tagtgtcttt gagcttttca gagagtctcc
     1321 ggccggtgtg aggggacagg ggagagggca tgaagttcat tcctgaactg tacggctgaa
     1381 cgtgagtagg ctgtaccggg gcggctgccg ttatacggcc atgagttgat cgattcgaga
     1441 taattagggt ggtaccgcgg aatcttccgt ccctggcgta ccactctttt ttttatccat
     1501 atatcgataa attgggtggt accgcgactg agtcgtccct tttgtgaggg gcggcttttt
     1561 tttcgggaaa ttattttaga gaggagtttt tactatggga actacaggga ttcaagtctt
     1621 cattatttta gtgtacatcg gcgttctctt cggtatcagc tggtacgtca agcataaggc
     1681 tgccgcatcg gcagaagcgt atgtgctggc tggccgcaaa ttgacgacgc ccctcatcat
     1741 ggtatccatc gtcggcctgg ctgtcggcgg ggcttcgacc atcggcgttg ccgaacaggc
     1801 ttataaagtc gggctgtcgg ccggctggta tacggcggcc tggggcatcg gcgccatcgt
     1861 catgggcctg acggtcgcca agaagtatcg ccagctcaat attacgacgg taccggaact
     1921 gctggaacgg tattatgacc gccgcagcat gatcgccggt atcggctgtc aggtcctggt
     1981 acagctggtc atcatgagcc tgcagtacgt cgcaggcggc gctatcctgg ctgccctgat
     2041 gccgaatatc ttcacgccgg tcacagggat gctcaccagt gccgtcgtct tcatcggcat
     2101 tactcttatc ggtggtatgt ggtcggccag ccagtctaac gtcctcaatg ttaccttgca
     2161 gtacatcggc attacggttg ctgctttcct catcctgggc atggccggcg gcatcgacgt
     2221 cgtcgctatc caggctccgt ccattacgtc catggacctc atcagcggtg tcggtcccat
     2281 gaccatcatc acttggatcg tcgtcctcat tacggtcaat gtatcgctcc aggctatcat
     2341 tcagatttct ctcggtgcca aggatgtcca gacggcccgc aaaggtttca tcattggtgg
     2401 tctggtcatg ctgcctgtcg gtttcatcgc cgctctcctc ggtgtcatcg ctgccgaatt
     2461 ggatccgtct atttctggta cgaatgcact gcggtagctc atcatgtccc tcaatccctg
     2521 gattgccggc gtgacactgg cttcgctgtg ggctgctgat gtcagcacgg cttgcaatct
     2581 cctgctgtcg gctgcgacgc tgtattccca tgatattcat aaacagttca tcgaccccaa
     2641 tatgtccgat aagaaataca tgaccgtaac ccgtatttcc gtcctgctcc tgggcctgtt
     2701 gaccttgggc ttcgccctga ccatcagcgg catcatttcg accctcatgg ccggcttgtc
     2761 gctcatgacg gctttcgcag tcatcgtcct catgaccgtg tatgcgccga aatactgctc
     2821 ccgtcaggct gcgttctaca ccatcctggc ttccatcatc gtcctcgtcg cctggatgtt
     2881 cgtaccggct gtccgcgtac tgccgcacgt catttatgcc gaatggattg tctgcgccgt
     2941 caccttctgc ggcatcagct tcgtatccaa gaaagccatc gcaacggacg gactcgtaga
     3001 agaaacgaaa cctgtcggcg caccggcaca gcattaggag tatcggccgg ccgtctgctt
     3061 attgcgcaaa tctgaccggc tctgcacgct gggactgcgt tgtcgtcagt cgacgtagct
     3121 atggctacgc ctccttcctc tgccttgccc cagcgcgcat atccagccat cttagcgcaa
     3181 acgcaaccgg tcggccgata cggcgattaa actaaaaact acaaacttaa aaagaggctg
     3241 tcttaatgca acagcctctt tttttgctcc cctgaggggt tgtttttaaa tcaccgcgtg
     3301 gaaattcaac ctctccggcc cgttgggcca cctcccctat gaggggaggc ttgcgacctg
     3361 cgacctgcgg gccgctctgg cggcccttct ctcatcccca caaggcgttg ccggtggcga
     3421 agccaagcca gcagcagacg aagccgccga tgacgttgaa cgagaagtag gccagggctg
     3481 ggaagacggc gccagtacgg acgaaggtga ggagttccat gttgtacgtc gaaaaggtcg
     3541 tgaagccgcc caggatacct gtgacgaaga agagtttgac gatgggcgac aggtcgctgc
     3601 tttcggagaa aaagacgaag aggatgccga tgcagaagga cccgatgagg ttggccagga
     3661 tggtgccgta tgggaagaat acgccgccgt ggaggttggc cagttctgtg agtaaatagc
     3721 gcagggctgc gccgatgccg ccgccgaggg cgacgaagag gactttttcc atgaatagga
     3781 ctcctttttt tatgaaataa gcgggccgcc ctttgggacg gcccctacgg aagcggtccg
     3841 tgggaccgcg gcggcctgag ggggtctttc aaaaggctcg cctcatagga gagctgtcag
     3901 cgcagctgac tgagaggttg cttttgaaac gcgacctgcg gtaattcaac ctctccggcc
     3961 cgttgggcca cctcccccta taaggggagg ctttgcgacc tgcggcctgc ggcctgcgaa
     4021 catcaattaa taccataaca cgatgggagc ggcgatgcaa gacacggctg gcccatcggg
     4081 acattctgtg atacaatata ggatacctaa cgaacgatga atgaaaggag ggggatgcat
     4141 gagctggcaa gtagagaaag cgctggatga tctgatggcc caggaaacgg gggctgtaca
     4201 gtatcctttc ggcacgcgtc atgccatggc tatctgttat cccaatacgt atgacgtggc
     4261 catgagcaat ctggggatgc agatcatcta ccgcgaagtg aacggccgcg aggactggct
     4321 ctgcgaacgg gctttcctgc cggacaagga cctcatgaag gtcttcgaga agacagggga
     4381 accgctcatt tccctggaga accgccggcc cctgtcggat ttcgaaatcc tcggtctgtc
     4441 ggtcagtttt gaaatggact atttcaacgt gccgacgatc ctggaaatgg ggcgcatccc
     4501 cgtcctggcc gctgaccgcg atgatgacga tacgctcgtc gtcatgggcg ggcctgtcgc
     4561 ctttttcaat gccgaaccgc tgtcgccttt tgtcgacgtc gtcctggtcg gcgaagggga
     4621 gcgattcatc cataccttcc tcgacgccta tggagaaggt aaggccgagg gcctgtcccg
     4681 tcacgacctg cttcgctatc tggctatcca tgtccccggc gtctatgtgc cgtccctgta
     4741 ccgccatgaa tacgatgacg acggccacgt cgtgcgcatc gttccggaag agggcatccc
     4801 ggccaaggtc gaacgccaat ggcacgagct gacccagccg gcggaaacgg tcatcgccac
     4861 gccgaatacg gagttcggcg ccatgtacct catcgagatt gcccggggct gcggccgtca
     4921 ctgccgcttc tgcatggccg gctactgtta ccgccgtccc cgcgtccgtc cgctggaata
     4981 cgtcaagtcc gccgtcctgc gcggcaaggc cctgggcaag aaaatcggcc tcatgggcgc
     5041 ggccatttcc gattatccct atatcgacga actggtcacc ttcatccgcg accaggggct
     5101 ggccttttcc tgtgcgtccc tgcgggccga ctcgattacg ccggccatcg tcaagggcct
     5161 ggccgaaagc ggccagaaga cgattaccct ggcgccggaa gcgggcagca agcacatgcg
     5221 ggacatcatc aacaagggca tcacggaaga gcacctgctc cattccatcg acctggcgac
     5281 ggcagccggc atccgccacg tgcgcatgta catcatggtc ggcctgccca tggaaaccga
     5341 tgatgatatc cagggcatcg tcgatatgac ccgccgcgtc cagcagcaca tggcggccat
     5401 cggcaacctg agccgcatca ccctgagcat caaccccttc atccccaagc ccttcacgcc
     5461 tttccagtgg atggccatga cggaaaagaa ggtcgtcgag aaacggctga atttcctcaa
     5521 gaaggccttg aaggacaagc agatcgagct gctcatcgag cccctgcgct cggcctatat
     5581 ccagggcgtc ctgtcccggg gcgaccgccg cgtcggcgac ctcctggtca aggcccatga
     5641 atacggcggc gtcaagggct ggaaacgggc ggccaaggag cttcatttcg atattaaagg
     5701 atttttatat gaccagcggc cgacagacgc cgtcatgccc tgggatacga tcgacacggg
     5761 actggtgccg acgtacctga cggacgagct gaaaaaagcc aaagacgggg aattcaccat
     5821 cccctgtttc gacggctgcc gacgctgtca catttgtgga ggatatcatg actaagtggg
     5881 tagaacatgg taatggaatc tgttgggaag agagcaccct cctggcagat gccggcctgc
     5941 gccatggcgt gaccggcaag agcggcggcg tcagcgaggc cccgttcacg tcgctgaacc
     6001 tggggctcca cgtcggcgac cagcccaagg ccgtcctgga gaaccggcgc cgcctctgtg
     6061 ccctcctggg ctatcccctg cagaagctga cgacggccca gcagacccac gaagaccacg
     6121 tcgtcgccgt gggaccggca gagatcggct gcggtgccgg cagttatgcc gatgccctgg
     6181 cccatacgga cgccatcatg accaatcttc ccggcgtgcc gctcatgctc tgcatcgccg
     6241 actgcgtgcc ggtcatcgtc taccatcccg tgacgcgggc cgtcgccgtc ctccccgccg
     6301 tctggcgcgg gaccgtccag cg
//
LOCUS       DAWCYS010000077         6307 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_741_length_6307_cov_8.370282, whole genome shotgun sequence.
ACCESSION   DAWCYS010000077 DAWCYS010000000
VERSION     DAWCYS010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_741_length_6307_cov_8.370282"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1294 a         1755 c         1795 g         1463 t
ORIGIN      
        1 tcccgcccat ggaaatggtg gcattctggc agaaacagca gcgcaggttg ccgcccgtga
       61 aaaagacggc gccagagccg tggctgccgc tgatacaggg ttcttcccac tggtgcaggg
      121 ccgcccgggc tacgacgggc tggagcggac tgtggcaaaa gccgggcatc tttttttcat
      181 cccaaaaggc cgcgcggacg acgccgcagc ggcggggaca gtcattacaa taggtttcca
      241 tagttttcta tacctcagtc aaaaattttt ccgggattca ggatattctt cgggtcccag
      301 gcctgcttca cagccttcat caaggccagt tccaccggat ccgtatactt ggcgaacagg
      361 gccttgcggg cctgaccgat accgtgttcc ccggaaatgg caccacccag ctcataagct
      421 gcctggcagg ccttatcttc aaaggcggcg actttctgct tttcatcccc ttcatcggcg
      481 aagcccttgc gcaggacatc gagatggata ttgccgtcgc cggcgtggct gacaccgcgg
      541 aagacgacgc ccagcgcctg tcccaaatcc cgtaagcgtt ccatgaaggg caggagccgg
      601 tccggcggga cgacgaagtc ttccatggcc gccacggggc agtccgcagc cgacgcttcc
      661 gtaaaggcct tgcgggcctt ccagacttta tcggcgtcgg ccatgaagac atcgacagct
      721 cccaggcggc ggcagacttc atcgacagtg acgcagtcgt catcgagcgt ttcgtcccgc
      781 gagctgtcga gctggataat catataggcg ccgccggccg ggcagggctg tttttctttg
      841 agccagtcct ggacgacgga aatgacgtca tagtccatga attccaggca cgtcggcgta
      901 atacctgcct tgggcagggc cgtgaccagg gccagggccg tttccggcga cggaaagacg
      961 gccaggacgt gggccgtggc cctgggcagg ggtacgagct tcaaagtcag ctccgtaatg
     1021 atgcccagcg tcccttcgga accgatgacg attttttcca ggggataacc ggccgtcatc
     1081 ttgtgcaagc gcttgccgag atgggcgatc tgcccggtcg gcgtgacgac ttcgatggcg
     1141 tagatctgat cccgcgtcgt cccgtacttg acggcccggt tgccgccggc attggtcgcg
     1201 ccattgccgc caatacagca gcagtcgccg ctgcaggggt cgccggcata gaggaggccg
     1261 cgcttgcggg cttcttcctg tatccgcgcc gtaatgacgc cggcctggac gaccatgtac
     1321 atggcgtctt cattgatttc tacgatacgg tccatccgtt ccgtactgac tacgatgccg
     1381 ccgcggctgt cggccacagc tccgccttcc aagccggtcc cggtccccct ggggatgacc
     1441 gggatgacgg cgtcattggc caggcggacg acagccgcca attcttcggc cgacgccggc
     1501 aggacgacga cgtcgggcag acaggctggc ccggtccctt cgtcttgtcc atagggaatc
     1561 tttttatccc ggtccgtcca gacatattta gcgccgacga tggcttcgag gtccgcgacg
     1621 acggccgggg ttacgtgatt atactccata atgacttcct tctcaggctt cctgcccgaa
     1681 gataaatttt aaataacttt cctggaactg gcgatatttc cgcttggcca ggggaatctt
     1741 atggccctgg atttcgatgt aatcgctctt gatgacgcgg atagccgact ggttgacgat
     1801 atagcctttc ccaggcgata cgaactggcc cggcgcaatg gcatccagcg tatccaagag
     1861 actagccagg gactgccgcg tttccagcag caagtggtct gccgtataga cctgctggat
     1921 gtgcccattg ctttcgacgt agaggatggc gttgacttct tcgacctgga taccgccgcc
     1981 gacgaggcgg aagatcatct tgccgctgtg gcgctggcgg atgctttcca tgacgcggtc
     2041 catggcctgg tcgaactctt tctgcgtaaa gggcttgacc aggtaatggg cagcccggac
     2101 ggcaaaggct tcgacggcaa attcttcgct cgtcgacaag aagacgattt ccgcccggct
     2161 cttctgcttg cgcatctctg cggcaatgtc cgtccccagc aggccgggca tacagatatc
     2221 cagcaggacg atgtcgaaat tcccttcctt ttcaaaagca tcgacgaaat ccatggcatt
     2281 gttataggta aagacttcgg tcggttcctt tttatcgtgg aaatactgtt ggactgcctt
     2341 ttgaatgatg tccaactgtt cctgtaaatc atcacaaatc gcaattcgta ccatcgtgac
     2401 gcctcctttc aataaacaag tattttacaa gtttattata tcaccagatt cgtctcaagc
     2461 cgtccgatgt gtcacgaaat ggcgcatttt tgacgcgtag cggcgttcag aagaaggtat
     2521 ttaccatcat gcatggtccc tttttctcta tagtaggggc gcccacgtgg ggcgcccgtc
     2581 agcatatgca agaatcccgt tatcactgat ttgcctgagc gggctcgcca cgtgcgagcc
     2641 cctacagaaa agggactgtc gcacatgcaa cagccccttt tcccccacta cagtacgatg
     2701 ccccgcagga gcggtacgat ggtgaactga tataatttca aaaagccgaa gacgcagatg
     2761 ccgatggatg cgccgacgat ggccccgttg atacggatcc aggccaggtc ttcgccgact
     2821 ttgctttcca ggaactggtt gaaccgcttc ttgtcgaagc ccgacaggac gtcgcggacg
     2881 gcgacggcga tgagcgagtg ttcatgttcc aggatgacgt tgcaggcccg gcgtatctgt
     2941 tcatcgagcc agtctttgat ttccggatag gaccggcagt attccaggta atggatgaac
     3001 tgttcttcca ataaatcctt cagttcttcc ttgccctggt cgcctttggt gaaatagtcc
     3061 ttggccgaat tctgggtcgc cgtcagccac tgctcgatgg gcagggagtc gacgaactgg
     3121 tcctgccagt tctggacgcc aaaggccagc tggccgttca tggaatagag gaagagttcc
     3181 agtttgcggc acagccattc gcgctgttcc ccttcggcat gttcccactg gtcgagccat
     3241 tcgacgatgc ccatcttgat atccttggca atcttacggg tatcgacaat gcccaggact
     3301 ttggtcagtg tgaggctgga catggacgaa tgctgcgatt cttcacgcca ctggaggatg
     3361 agcttttcca ggaggctgtc gaagtcgtcg ctgttgacca gttcccgcac gaggccgatg
     3421 gcatcgttga acatgttgct gttcgggtct tccaaaaggg aaatgaggcg gtcctggaag
     3481 aaactgacca ggggctgctg tttcaggtac ttgcggatgt gcgaggctat ggatgcctgg
     3541 gtcccaccct tctgagcgta gcggtaagcc cgctgagcca cttcgtagag gacgctgcgg
     3601 aacttttccc ggccgttcgg gccgcgcagc cagccgacga gcttatcgat gaaagaaatc
     3661 tgatagagct tatcctgcca catattgggc tggagaagct tttccgagat gatcttggtc
     3721 atgctgtcga tgatctggtc gcgatggcga tagagcagtt ccgtgtgata cggaaagccc
     3781 aacggctttt caaagagcgc cgtaacggcg aaccagtcgg cgatactgcc gatgagggcc
     3841 gattggatga cgaagaacag cagttcttcc cagaccgaga cgtcatgcgt ataccaccag
     3901 aggaaaatgg cgatgaagca gacgaaagac aggcctaaaa tcatattggc acgctgtctg
     3961 tatgtcataa gatagcccct cccttcacgg cgtacatgac caggtaaatg acggcaccca
     4021 ggatggcgcc gacgagcgaa ccgttgacgc ggatcatctg caggtcatac cagccactgt
     4081 tttccagctg ttctgccagg gaattgccgt cgtatttcag catttcctgt tctgccgtct
     4141 tgccgaacca tacctggagg cgcttcaatt cgcgggccgc aaaggccagg gcctggcggt
     4201 tcatgtgttc cacctggtct gggctctggc gccacgattc cagtttattg atgacgtaat
     4261 tagctgcgac cttgcagacc cgcttctgcc gttccgggtc ctggacgtag cgctgccagg
     4321 cttctttcgt gtccggccgc gtgaccaggc tgttgtacat gcggttctta tagctgtcga
     4381 tgcgctggcg ccattcgtcg ttgaattcga tcttttccgt ctgtttccac aaatagcgca
     4441 gggcctgctg gcgctggagg gaattgtctt ccttggcttc ggccaggagg tccaggcatt
     4501 tcgtctgcaa gaggccggct acgtgatccg gctggaagac ttccatcttc aaggccgcct
     4561 tgatggccca gccgaggaag atattgtgct tggcgtactg gcgcagggag gcttcgtaga
     4621 tttcaccgac atagcgcttc atcgtttcgc tgcgcacgag gtcttccagg ttgaggatca
     4681 tgaaatcgag gaaatccttc cccgattcac cgtgcagacc gattttgatg gccttgctca
     4741 tgatcggtgc caaatcgact ttctcgatga cctggccgcc gatgccggca aaggccttga
     4801 cgacggtctt catgtcgatg gtgtacaaga aggtattgag gatctgaccg aagacgcgtt
     4861 ctgccgactg gaaccccttt tccgtatgga gataatccag cgtcgcgccc aggatgggat
     4921 gatgtttgag gacgttgtac atattggaaa cggtcaggat ttcatattcg atcatatgcc
     4981 gggccatttc ggcaatgcgt tccttgctgt tcggaatgag ggccgtctta aaggagatgc
     5041 ccaaaggccg gcggaacagg gccgttacgg cataccagtc tgccaggccg ccgatgaggg
     5101 ccgccccggc cagatgggtc agcagtccgc cgacaaaaaa gtcttggaag gggtatgcca
     5161 gaagcaggag gactgccgcc gtaatcagga tataattggc aatctggctg cgtctgcccc
     5221 cgtgtgtatt acttgccatg atacctcctt actgcccgcc gatgatgacg tcgtcgccat
     5281 catgctgggg aatccacaag ggtacgtccg gataagcctg tttaaaagct gccgacggcg
     5341 tccagggcgt gccgaaataa tgcatgggga tgaggcattt cgtgaccgaa acgtctttca
     5401 ggaaacccgt gacgccccat tcgcgggcgc cttccaggcg ggcgtcgaca gggaaaaaga
     5461 caatgtcgaa agtctgtccg gcaaggtgtt tcatttcccg gtcgaagttg ctcttggctt
     5521 cggcattatt ttcatccgta tcgccgagcc agtgccacca gttcaagtcg ccggcatgga
     5581 aaatcttcaa gccgtccgtt tccaccagga acgacccgcc ttcatcagtc gacccatact
     5641 gggtcaccgt cgtatctccc aggggcagcg tttcatacaa cttcatggca tggaaccggt
     5701 ctgccggaat gtccttgagg cgcacatctt cattgacgat gtactgggca ccgccgaact
     5761 ggccgatgct gcggttgaaa tggtcttcgt gccaatggct gacaaagaac cacaacggct
     5821 tcctgccgac ctggctgtaa gccttgaccg tccccgatgg atccttgtag taatcaaaga
     5881 ccagcacctt ggaatcggtc tcgacggcaa agccgctgtg attcaaaaag gtaatctgta
     5941 cactcatata gtaaccccct tatcggttgt ttaatggcta ttgcatactt tttattatac
     6001 catatatgcg tcgcccgtgt acatgccggg gagggttgtg gctaggggct ggtggctcgt
     6061 agttcgtggc tcgtgcgaat ttctccaatt tttagttgta cgggctcgca cgtgcctttt
     6121 cagcccgtca ggccatgact acaaggaatg gcaggacggt tgctgaaacg gaccactgta
     6181 gagggcgagc ccgcttgagg atatatgtga ttcccccagc cagtttgcta gggaactcat
     6241 ccaaagttct aacgggtcgc ccacgtgtgt tatgtgcaat aacatagttt acataaatag
     6301 gggtctt
//
LOCUS       DAWCYS010000078         6263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_746_length_6263_cov_9.270457, whole genome shotgun sequence.
ACCESSION   DAWCYS010000078 DAWCYS010000000
VERSION     DAWCYS010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_6263_cov_9.270457"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1563 a         1595 c         1763 g         1342 t
ORIGIN      
        1 aagtacttgg ggggaggccc cctgggagga taggaagccg ctgattaaga aaaaagcact
       61 ggaaaattcc agtgcttttt tgctattcgt aggggccgtc ccaaagggcg gcccgcctga
      121 aatgtgatgt acacgccata catgacgggc tcggactcag atgccgctgc acggcgacag
      181 actcaaactg acaacatcgc ttcgcgtaac cctcagctcg taaaaacgcc ccgaccgggg
      241 cctgcgtagg ggccgtccca aagggcggcc cgccgcgaat aaatggtgat atacataaca
      301 ggtccatgag gcatggtttt acgagccacg aatcacgagc aacgagccac ggcttttttc
      361 tcggattcag atgcggcgtt ccgccgcgac agactcaaac tgacaacgac gcttcgggaa
      421 aacctcagcg cgtagcggga cgccttgaca ggacgtcccc tacgaaagcg gtccggtgga
      481 ccgcctgctg actgcgacct gcgaccggcg gcctgcggcc tgcgaatggc gttttctccg
      541 aacagcattt cgcgggattc cctcaacggg acgcctagcc acagctgtcc gttttggcag
      601 ccgtcctgcg cctcctgctg gtcggcacct gacgggccaa aaaggcttac aggacgtccc
      661 ctacgaaagc ggtccagtgg accgcctcga ttcggcagga gaggtgggaa aatagtggct
      721 agtggttcgt ggctcgtggg acggccgttg ctaacggcta actgctgacc gctaaccgct
      781 aactgcgacc ggcggcctgc gaacatactc aattataata acagcgattc tcataaagca
      841 ttatgcgaaa ggagactaat attgtataat atagctgtaa agatactgaa attatggtat
      901 tcgctatata ttactttcga caggggaggt tttactatga aagatatccg tattgaacaa
      961 gtcgttggcc gggaaatcct tgattcgcgg ggtaatccta cggtacaggc cgatgtgttg
     1021 ttatccgatg ggactttggg ccgcagcgcg gctcccagcg gcgcttcgac ggggcagttt
     1081 gaagccctgg aattgcgcga tggcgatgct cagcgctttg gcggcaaagg cgttagccag
     1141 gccgtcaatc atgtcaatac agttttacag gatgtcttga gagggcagaa tccttttgat
     1201 acttacggta tcgacaacct gatgattcgt gccgatggga cggaagataa atcgcggctc
     1261 ggtgccaatg ccatcttagc cgtatcgctg gcctgcgccc gtgcggcagc tgcgtccctg
     1321 gggattcccc tctatcgttt cctcggcggc gtcaatggta attatctgcc cgtcccgatg
     1381 atgaatatcc tcaacggcgg ggctcatgct gccaataccg tcgatgtcca ggaattcatg
     1441 atcatgccgg caggcgctcc ttccttccgg gaaggcctgc gctggtgctc cgaagtctat
     1501 catgccctgg ccggtctctt gaaagccaag gggctggcta cttccgtcgg tgacgaaggc
     1561 ggctttgctc ctgatttggc cagcgacgaa gaagctatcc aatatatcct tcaggccatt
     1621 gaaaaagccg gctatgaacc gggcaaggat ttcgtcctgg ccctcgatgc cgctgccagc
     1681 gaatggaagg agcccaaacc gggcgtttac gttctgccca agagtggcca gtcctttacg
     1741 acggacgaac tcatcgccca ctggcagaaa ttgtcccagg cttatcccat ttattccatc
     1801 gaagacggct tggatgaaga agactgggat ggctggacgg ccttgacgaa agccttgggc
     1861 cagcgcgtac agctcgtggg cgacgattta ttcgtaacca acacgaagcg tttggccaaa
     1921 gggattgccc agggctgcgg caatgccatc ctcatcaagc tcaatcagat tggctccgta
     1981 tcggaaacgc tggaagccat caagatggcg caccgtgccg gctatcgggc catcgtttct
     2041 caccgttccg gtgaaacaga agatacatcc attgccgacc tggccgtcgc cttgaatacg
     2101 gctcagatca agagcggtgc gccgaaccgc agcgaacgcg tagcgaaata caaccgtctc
     2161 ttgcagattg aagacgaatt gcgcggccag ggcatctatc ccggattcca ggctttcgga
     2221 attcagcgct gaacgtagtg atcctgtccc cacgtacaaa cttcatgtag aataggcatc
     2281 aaagatttcc ccagttccgt tagtccatat tcgacctttg gcggtacctg gggatattct
     2341 tttctataga gaatgccgtc ggcttccatt tctttcaaaa ggatgtggta tcgattatga
     2401 aattttccag ttagggcccc ggccgatccg cgactgcatc ggctgtaata aatgccaggg
     2461 taagggctgt atctttaagg atgacaatga tgataccgtc aaccgcttct tggaaaaggc
     2521 gaaggaagct gatggcttcg tcttcgggac gccggtctat tttgcccacc cgagtggaca
     2581 gatcctgtct ttcctgaatc gcgtcttcta ttcgtcggct gtaggcgagc tctacccggt
     2641 ctttgccggt aaacctggcg cagcgattgc atcggcccgc cgcggcggta ccacggccgc
     2701 tgtcgatgtc attcagaaat atttcggcat tgccagcatg cccatcgtta cctcgacata
     2761 ttggaacatg gtccatggca tgacgccgga cgaagtgcgc aaagatttgg aagggatgca
     2821 gaccatgcgc aacatcggcc gcaacatggc ctggatgttg aagggctttg ccaaacagga
     2881 aaaagaaaca tcctttgctg accacgtaga agcaacacat cgcacggact tccaccatta
     2941 aaatatagat gagatgcagt tcgcaggccg cggttcgctc ggattcagat gcggctgcgc
     3001 cgcgacagac tcaaactgac aacgacgctt cgggaaaccc tcagcgcgta gcgggcctcc
     3061 cgctgggctt gctcctacgg atgcggttgt gcggcgacaa tctcaaactg ataacatcgc
     3121 ttcgagtacc cctcagctcg taaaaaatgc cccgaccggg gcctgcgtag gggccggccg
     3181 ggtaaggcgg cccgtagcga acagtcgatg atatacgtaa cgggttaatg aggcatggtt
     3241 ttgcgaactg cggtctgcgg gcctgcgaat ggcgttttct ccaaacagca ttttacggga
     3301 ttcccacaac gggacgcctt atcaggacgt cccctacgat atcgcataca ggcgatggaa
     3361 atggtatgat gtttgatgta atagctttaa atttaaaacc agccactaac aactagccac
     3421 tagccactca aaaaggagct gccgcgatgc gacagctcct ttttcacttc cctttattct
     3481 cttacaatgc tgtgtttacg gctgtaggcg tagtagacga tgaggcctaa gagaatccag
     3541 cctgcgaaca ggatatgggt cagtgtcgac agttcataga tgaggtagaa gcagacgatg
     3601 atgccgacgg gagcgatgag gtagagcagg gggcaatgga atttccgcgg gatttccggg
     3661 tgtttgcggc gcatgacgac gatgccgatg aagttgacca ggaagacggc caaggtccct
     3721 gtattggaca attcgacgat gtgcatgagc gggacgaagc cggaaatgac ggcgatgagg
     3781 atggaacccc aaatggtgat caaatacggt gtgtggaagg tcggatgaat cttgcagagc
     3841 ttctgcggca gcatgccgtc gcgggatacg gcaaagaaga cgcgggactg accgaagaga
     3901 tagacgatga gcgtcgtgat gataccggcc aggacgccga cggcgatgag gttagccatg
     3961 ccgggccagc cgaggagacg cagggcatag gcaaccggtt ccggtgtatc gagctgggtg
     4021 taattgatgg cagctgtcag cgacagggat acggcaatgt acaaggtcat acagatgacg
     4081 atggaggaga tgatgccgat tggcaggtca cgtgacgggc acttacattc ttcggctgac
     4141 gttgcgacgg cgtcgaagcc catgtacgag aagaagacga tagccgcccc gctgcagacg
     4201 ccgcggacgc cgaagggcgt gaagggcgat aaattagctg cctgggcgtg aggcgctgcg
     4261 atgacgacga aggcgatgat agccgctaaa gcgacgagga taagggcaaa attgagtttc
     4321 gcactctgtt tggtaccgcg gatgaggact aagcacaata aaaggataat aatgatagac
     4381 ggcagattga ccaggccgcc ttcagccggc gttttgagga gataatccgg gattaccagc
     4441 cctaaagagg ccgtcatccc cgacaggtag cctgagaaac cgacagcgac ggcactggct
     4501 gtgaccgtat aggccaggag cagggaccag ccgcagagga aggccatgcc ttcgccgagg
     4561 gaggcaaagg tgtaggaata gacactgccc gaagccggga tgaaggagga aaattcactg
     4621 tacgtcaggc cgacgaggat acagagaata ccggcaatga caaaggataa ggctacgccc
     4681 gggccggcat agcgggcggc ggcaatcccg gtcaggacga agacaccggc cccgatgata
     4741 ccaccgacgc cgaggaatgt caggtcaaag acgctgaggt ttttgctcag ctttgagttt
     4801 tctgcatcgc tctgaagctg ggcaacggat ttcgtatgga aaaacgacat aaaacacact
     4861 cccttttttt aataaaaaca atgaaaaagt taaattgtat aaactttttg ttaaatacag
     4921 tgtactatga tacagtaagt atcgtcaata tgttttataa atatagattt gtgtatacag
     4981 tatcctttat caccaatctt gcaatatcgg caagatacgc tataatattg atgatgagaa
     5041 ttcataataa aatgaaacag agggggcgtg acttatggga tttttcagca atctcttcca
     5101 tcacgatgat attcagggga cgaaaaaata tatctacatt accggcatgg tctgccagaa
     5161 ctgctctaag cacgtgaccg aagcccttaa agccgtcgac ggctttaagg gcaaggtcaa
     5221 agttgacttg atgcgcggcc aggcagaagt cgtcgtcgct gattccgtga gcgatgacac
     5281 gttgttaaaa accgttgtca atgctggcta tggcgtaacg gaaatttcct tgcagccaaa
     5341 gaaaaattaa aatataaaat agtaagttga taaaaataaa atattatagt ataatcggct
     5401 tgtaaattaa atttattcat gacgatgaag tcttacccgc aagtggataa gactcttttt
     5461 tattcccagt ataggagtgg tgttatcgtg tcttttcaga tgtttgtccc tacgaaagcg
     5521 ttatttggtg ccggtcagct caatcatttg catgaacagg cccttcccgg caagaaagcc
     5581 ctgctggtca tttccaacgg gaaatcgacg aaagtcaatg gctatttgga ccggacggaa
     5641 aaggaactgc atacagccgg cgtcgaaacg gtcctcttcg accaagtcga agcgaatccc
     5701 ttgaaatcga ctgtcatgcg cggcggcgcc atggccagag aaaatcaatg tgacttcatc
     5761 gtcgccttag gcggcggcag ctgcatcgat gcagccaagg ggattgccgt cgtcgccacc
     5821 aatgacggcg atttgtggga ctacatcacc cagggcacgg gcaagggcaa acccattgcc
     5881 atcaagcccc tgcctatcgt cgccattacg acgacggccg ggaccggctc ggaaaccgat
     5941 gccggcggcg tcatcaccaa tgaagacacg aatgaaaaga cgcccgtcaa aggaccatcc
     6001 ctcttcccgg tcctggccat catcgacccg gaactcatgg cgacggtacc accgaagttc
     6061 acggcctacc agggtttcga tgccttgttc cacaatatcg aaggctatct ggccaataag
     6121 gccaatctca tgagcgaaat ggtcgccttg gaagccatct cccagattag cgcctacttg
     6181 ccgacagccg gccagaatgg catcgacagg gaggcccggg accgcgtggc tttcggcaac
     6241 tatctcggcg gcgtggagat gtg
//
LOCUS       DAWCYS010000079         6227 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_754_length_6227_cov_7.189080, whole genome shotgun sequence.
ACCESSION   DAWCYS010000079 DAWCYS010000000
VERSION     DAWCYS010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_754_length_6227_cov_7.189080"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1492 a         1760 c         1741 g         1234 t
ORIGIN      
        1 agcatcacct tgccaaggtg ggggtcgcga gttcgagtct cgttttccgc tccatatatg
       61 gcggcatagc caagcggtaa ggcagaggtc tgcaaaacct tcatccccag ttcgattctg
      121 ggtgccgcct ccatattttc atctttcgtc atgccgaggt ggcggaactg gcagacgcaa
      181 cggacttaaa atccgtcgga tagagatatc cgtaccggtt cgattccggt ccccggcacc
      241 aaaaaaacaa cgagcgtgtg agcggcgttg gccgtggccg acgagtccct tgcaaaagaa
      301 aggagctgtc ttaggacagc tccttttcgc agtccagtga aattcacatt catgggaaaa
      361 tgagttataa tgaatcatga cagaatacaa gtataaagta tcggattttg aaggtcccct
      421 ggacctgctg ctctatctca tcgaaaagaa caaagtcgat atttataaca tacctatcgt
      481 cgagattaca gaccagttca acgcctacgt agcggacatc gacacattcg atgtcgatta
      541 tgccagcaag ttcttcgtca tggcagcgac gctgttgcag atcaaatcgc gcctgctgct
      601 gcctaagcag ccgaaactgg ctgaagagga ggaagaagac ccgcaggaag cgctggtccg
      661 ccagctcgtc gaatataaaa agatgaaagc cctgtcccag gctatgggac agttgtggga
      721 tgcccgcagc ctcatgctgg ggcgcctgcc gacggccatg ccctatgagg cccgctttgc
      781 cggccacatc gacacagagg ccctctacca ggcgttccac aaggtctgcc aggcccttcc
      841 cgagccgaca gccgtagaaa tccgcataca gaaggaaatc tacagcgtcg aagagtgcat
      901 gcgccgcgtc acgtacgccc tgcgccgtgc ccaggggacg atggccgtcg acgccatatt
      961 ccggacctgc cggtcccggg tcgaactggt cgtcaccttc ctggccgtcc tggaactgct
     1021 gaaagttggc cagtgcgcta ctgttgctga aggagaggat gggaatacca ttgccttacg
     1081 atacacgcca aactgatacc atcgagccta cggctctgct ggaagctctg ctctttctga
     1141 gcggcgaccc catgacggtg acggccatct gcgcccatac gggctggacc caggccgaaa
     1201 cggaagcgac ggcagaccgc ctgcagcacc tgctcagcag ccagcggcgg ggtatcgtcc
     1261 tcatccgcgt cgccggcggc tttcagctgg tcacgcggcc ggacctccac gatgccctgc
     1321 aatgggtccg gacggggacg acggagttgt cctctatggc actggaagta ttggccatcg
     1381 tcgccttcaa acagcccatt acgcgggctg aaatcgaaaa gctgcgcggt gtctcgtcag
     1441 aacgggtcct gacgtcgctg gtccagcagg gcctcgtcgt cgaactgggg cgcaaggaca
     1501 gcccagggcg gccgatttta tacggcacgt ctgcttattt tttagaatgt atcggcatgg
     1561 attccctggc cgatttagcc ggccgcatgc cggcagatat tacggaggat gcaactgatg
     1621 gaagaacggt tacagaaagt gatgagtaac tgcggtgtcg cttcgcggcg cgcttcggaa
     1681 aagatgatat tagaagggcg cgtccgcgtc aacggcgtca tcgtccgcga actggggacg
     1741 aaagtcgacc ccgacaagga ccgcattgcc gtcgacggca agaccctgga agcgcagccg
     1801 aagcattatt atctgttcaa caagcccaag ggcgtcatca ccacgtcgag cgatgaccag
     1861 ggccgggcga cggtcatgga ctatttcagg aaggaaaagg accgcatcta cgccgtcggc
     1921 cgcctggacc agaatacgga aggcctgctg atcatgacca atgacggtga actggccaac
     1981 atcctcatgc atcccagccg cctcgtcgac aagacttatg aagtcaaggt caaagggcgc
     2041 attgccgatt cggtcctgca gcacctggca gacggcgtcg agctgaagga cggcatgacg
     2101 gccccggccg atgtctacta tattggctat gacccgaaga cgaacctgac cagtgtggaa
     2161 atcaccatcc acgaaggacg caaccgccag gtccgccgca tgttcgagtt cttcggctac
     2221 caggtccaca acctgcgccg cgtccagtac gccttcctga ccctgagcgg cgtcaagcgc
     2281 ggggcctatc gcaagctgac ccgcgaagaa gtggcggaac tctacaaata taaataaaca
     2341 ggagtgctta tgaaggaaat actcgtcatc ggtgccggtg cggccggcat tatggctgcg
     2401 ctggccgcgg cagaagccgg ggccagggta cacttatttg aaaaaaatga tatcgtcggc
     2461 aagaagatgg gcattaccgg caagggccgc tgcaatctga ccaattcgtg cagcatggcc
     2521 gatttcatcg cccatacgcc gggccacggc aaattcctct acagtgcgta tgaacaattt
     2581 accaatcagg atctgctgga taaattgcat acctggggcc tggctaccaa ggaggaacgg
     2641 ggcggccgca tcttccccca gtcggacagt gccatcgaag tgcgcaagct cttctatcgc
     2701 aagctctgcc agaagggcgt cgacctccat ctgagcaacg ccgtccacgc cgtcaaggcc
     2761 tggggcagcc gcatggtcgt cagtgcggcg tcggggaatt acgaaggcga cgcctgtatc
     2821 atcacgacgg gcggcatgtc ctatcccgtg accggttcga caggcgacgg ctacggcttt
     2881 gcccgcagcc tgggacacag cgtgacggag ctgaaaccgt cgctcattcc ctttacgacg
     2941 gaagaaacct ggcctcacac cctgtcgggc ctgtccctgc gcaatgtcga agcgtcgctg
     3001 tggaagcggg gcaagaaagc tgcctctcat ttcggcgaga tgctcttcac tcatttcggc
     3061 gtgtccgggc cgatcatcct catgctcagt accgaggcgg cccataagaa gtcctgcgtt
     3121 tttcccatgc agctgcgcat cgacctcaaa ccggctttgt ccaaggaaaa gctcgacgcc
     3181 cggctgcggc gggaattcga gaaatacatc cgcaacgaag ccgccaatgg cctgaaggac
     3241 ctcctgccgc agcggctcat ccccatcgtc ctcgaccagg ccggcatcgc cgctgcctgt
     3301 ccggtcaacc agatttcccg ggaacagcgg caggacctgg tcgataccct caaggccctg
     3361 agcctgaccg tgaccgggac gcgtcccatt gaagaagcca tcgtcacggc tggcggcatt
     3421 gccgtcaagg aaatcaatcc caagatgatg gagtcgaaac ttgtccccaa tctctatttt
     3481 gccggagaag tcatcgacat cgacgccttt acaggcggct ataatctgca ggcagccttt
     3541 tcgacgggct tcgtcgccgg aacggcggcc gctacaaagg agtgcatacc atgcgaaaat
     3601 tgacggttgc cattgatggg ccggccggag cgggcaagag cagtgtcgcc aaggtcctgg
     3661 ccaaccgggt ccattacttg tatatcgata cgggtgccat gtaccgggcc tttacctggg
     3721 ccgtcctgga aaaaggcatc gacctttctg atgaaaaagc ggtccgtgac ctcgtcgata
     3781 ccattgatat ccgcctggaa ccgcaggcag acctgtgccg cgtctacgtc ggaccgacgg
     3841 aagtgaccga agccatccgg acccagcgcg tatcttccca tgtttctgcc gtcgcggctc
     3901 ttgcagtcgt acgggaaaaa ctggtaaaat tacaacaggc catggccaaa gaaggcggcg
     3961 tcatcctcga cggccgcgat atcgggactg tcgtcctgcc ccaggcagac ctcaagatct
     4021 tcctgacggc gtctgtcgat tcccgggccc gccgccgtta tttggaagtc caggccaaag
     4081 gtggcagcga gacgtatgaa gaaatcgctg ccagtatcgc cgcccgcgac gatatggatt
     4141 cgcaccgcac cgtttcgccg ctgaagaagg ccgacgatgc catcgtcgtc gacaacagcg
     4201 atctcgattt agagcagacg gccgacgtca tcctgggcct gatgaaggag cgaggctgat
     4261 gcataccttt acatatggcg tgatctgcaa gattttcaat gtcgctacct acggcgtcct
     4321 gggcatggaa gtcattggtg aagagaacat ccccgaaaag gggccgttcc tggttgtctg
     4381 caatcatgcc agcaatttcg atccgctcct gctggggacg gccatgcgcc accatctgat
     4441 ccatttcatg gctaaagagg aattgttccg caatcccctc atgggctggt tcctgcgcta
     4501 tgtccacacc ttcccggtcc accgcggcca catcgaccgc aaagccgtca tcgagtcctt
     4561 tcgggtcttg aagagcggcg aggtcctggg gattttccct gaagggaagc ggacgcgggg
     4621 cggcaaagtc gggcctttcc atgaaggctt tgccggaatc gccatcaagg ccaaggtgcc
     4681 ggtcctgccg gcagctatcg tcaattccga attcctgccg aaaaagaccg gtcccgtccg
     4741 ggtcatcttt ggcaaacccg tcccggcacc gcagggaaag gcatcggatc gcgacttggt
     4801 caaaggtttt tccgacgaaa tccgcgatat tatcatagct atgcaaaatc agtataaagg
     4861 ggacgcaaaa tgaaagtaac tctcgccgcg gcctgtggct tttgctatgg tgtgcgccgg
     4921 gctgtcgagc ttgcggggca ggccgaaaag gggacgcata ccctggggcc catcatccac
     4981 aatccgcaag ttgtcgggcg tttagccgct catggcgtat cgccggtcga ctcgctggac
     5041 gatgtcgaca agggggctgt cgtcctgata cgctctcacg gagtcggccc ggatgtctat
     5101 gaacaggctg accaaaaaga actaaaagtc atagatgcga cgtgtccaca cgtcaaaaag
     5161 gcccaacaag acgctaaaaa catcgtcgaa aagggcgaaa aactcataat agtaggagaa
     5221 aaatcccatc ctgaagtaat tagcatttca caatgggggg caaatcatgc tattataata
     5281 gatggggaag aagaagctga aaaaattccc ttttgcgatt ctatgggagt cgtcgtccag
     5341 acgacgttct ctcaagagca attcaagcgt atcgccgcta tcctgcaacg aaagaccaat
     5401 catctcgatg tgcatatgac catatgcacg gcgacgcagc agcgtcagca ggcagcgata
     5461 gaactggcag gccatgtgga cgccatgatc gtcattggcg gcaagaacag cgccaacaca
     5521 ggtcgcctcg cgcaggtatg ccgtgaacag gattgcccga cataccacat cgaaacagca
     5581 gccgaattgg atacggcctg gttccgtggt atgaatcata taggcattac agccggcgct
     5641 tccacgccgg attggataat acaggaggtt gttaagatta tggaaaattt gcaagctgaa
     5701 ggcacagaag taatgagtga agaattgttg gacaagtatg actatgaaga aaatccaaag
     5761 aaaggtgacg tggtcaaagg cacggttatt tctgtgaatg atgatgctgc ctatgtttcc
     5821 atcggtacga aagcagaagc aatcctgccg aaaaaggaaa tggctgttcc cgctccggaa
     5881 aaagcaagtg actttgtcaa agtcggcgac gaactgactg ttgaaatcgc caacaacatc
     5941 aaagaagaag gagctatcgt cgtttccctc gtcaagatga aaaaagtcga agactggaaa
     6001 gaagtccgcg atgcttttga aaacgaccag ctcgtcgaat gtacaggtaa agaaacgaac
     6061 aaggctggcc ttgtcgtttc catcaaatcc ctgcgtggtt tcattcccct ttcccagggc
     6121 gatatcaaat tcgttaaatc tttggactat ctcgtaggcc agaccttcca ggtcaaagtc
     6181 atcgacatcg acgaacacaa gaaccgcctc gtcctttccc gcaaagc
//
LOCUS       DAWCYS010000080         6130 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_767_length_6130_cov_7.934815, whole genome shotgun sequence.
ACCESSION   DAWCYS010000080 DAWCYS010000000
VERSION     DAWCYS010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_6130_cov_7.934815"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1411 a         1971 c         1669 g         1079 t
ORIGIN      
        1 caggagttga gagacaaaaa cctgacggct gacgacgtcg tcggcggcct cagcgccgcg
       61 ggccggacgc cttatggcgt cggcggcctg acctatgcgg cctcggtcgg agccgctacc
      121 atcgccatcg actgttcgcc tcattcggcc atcggccgtt gtgccaacat cgacctctgc
      181 gccgtcgtcg gcccggaagt cgtcacgggc tcgacgcgca tgaaagccgg cacggcccag
      241 aagatgatcg ccaacatgtt gtctacaggc gccatgatcc gcctgggcaa agtctatggc
      301 aacctcatgg tcgacgtcaa gtcgtccaat cagaaattgg aagaacgggc gcgacgcatc
      361 gtcatgacgg cgacgggctg cagccgccag aaggccatcg aagccctcag ggaaagtcag
      421 ggccgggcca agacggccat cgtcatggtc ctcctgcaca tcccggccag cgaagccgaa
      481 gaccgcctga cagcggccca gggctatgtc gccgccgtcc tcaaggaggc tgcccatgga
      541 ttacgataaa ctggcacggg ccatctacga tatcaccggt ccggcaggca atatcgaaaa
      601 ggcttacaac tgcatgaccc gcctgcgcct gaccgtaaag gatgaacact ttagcaaaga
      661 cgacctggcc aaactgcccg gcgtcatggg catcaacaaa tccggtgacg aatggcagat
      721 catcgtcggc ccgggcaagg cgaccaaact ataccaggcc tttgccccct tagtcgaagc
      781 aggccgtaaa gaacagccgg cggccgctgc gccagatgcc ggcgacgata agcaggggcc
      841 cttcgacggc caggcccttc acgaagaaat acgccgcaag aacgcgacgc cggccaaact
      901 ggccctgaag aaaatcgccc atatcttcgt gcccatcatc ccggccttca tcgcctgcgg
      961 cctgatcacg ggcctgctca acgtggcctt caagatggac ccgtccctgg cggcccagcc
     1021 gctgatgcag gtcctggcca tcgccggcaa cgccgccttt tatggcctga acatcttcgt
     1081 cggcatcaat gcggctaagg aattcggcgg ctcgcccatg ctgggcggcg tcatggcagc
     1141 catcctcagc catccgaacc tggctcagat caatctcttc ggcgaatccc tggtccctgg
     1201 ccggggcggc atcatcgccg tcctcctggt cgtcttcttt tcatcgtggc tggaaaagaa
     1261 gctccaccgc ctcgtcccgg atatgttcga cctcttcgtc acgcccttcc tggtcgtcgt
     1321 catttcgacc ttcgtggccc tcttcctctg ccagcccctg ggcggctatt tgtcagaagc
     1381 catcggctat gcggcgacgg aatccatcgg gaccggcggc gccgtgacgg gcttcatcct
     1441 gggcgggacc ttcctgccca tggtcatggt cggcatccat cagggcctga cgccgatcca
     1501 cgcggaactc ctgtcgcgct acggcgtgac cattctcctg cccatcctgg ccatggccgg
     1561 cggcggccag gtcggcgcgg ctatcgccgt ctatgccaag acgaagaaca aggccctgcg
     1621 caagaccatc gcctcggccc tgcccgtcgg cctcatgggc gtcggcgaac cgctcatcta
     1681 cggcgtcacc ctgcccctgg gcaagccctt tatcggggcc tgcatcggcg gagcctttgg
     1741 cggcgccgtc caggccgcct acatggtcgg cgccgcgacg atcggcattt ccggcctgcc
     1801 tctggcagcc agtacggaca acatccccat gtatttatta ggactcgtca cagcctacgt
     1861 ggccggcttc attgcaacct ggctcttggg tttcgacgac ccgaaggagg gtgaataaat
     1921 ggaaacgggt atttccgtct atcccggcct gggagggacg atccaagaaa aaacggcccg
     1981 catcgaacgg gccgctgccc tggggatgac gcggctcttt acgtcattcc acatccctga
     2041 aacggacccg gcagccttcc gcaaggaact gcaggccgtc ctggacacgg cccggcaatg
     2101 tcagctcgac atcgtcgccg acgtatcgcc ggcctcggca gcctgcctgg gcatggaccg
     2161 tttcgatccg gaagttatcg ccaagctggg cattacaact gtccgctttg acgacggttt
     2221 cgatgcagcc aagctggcca tgtacagcca catcgtccgc gtccaggtca acgcgtcgac
     2281 gctgacccgg aaagacctgg aagacctccg gaagaatggc gcccagctgg accacatcga
     2341 cagcctgcac aacttctatc cccgtcccca cacgggcctg gccctcgact atttcaagac
     2401 ccagaccgac aggctccacg cctgtggcct gtccgtcggc gccttcgtcg ccagccaggc
     2461 cggccaacgg gcaccgctgt atgaaggcct gccgacgctg gaattccacc gccatcagga
     2521 cgtcagcctg gcagcccgcc acttggccgc cctgggcgtc cagtccgtct tcatcggcga
     2581 cgacaagccg gccgataaag aactgcgcga cctggcccgg gccggccggg aagaaacgga
     2641 cgtcgtcgtc atcaaggcca aactcctcag ctgggaaccg cacatccagg acctgttgtc
     2701 ctataccttc acggcccggc ccgacccgtc gcgggatgtc atccgcgcca atggcagccg
     2761 ggcccatctg gacggccgca tcatcgaacc ggacagcgac atcttccgca gtctccaccg
     2821 cggcgacatc accctggaca acgccgattt cctgcgttat atgggcgaag tccagatcgt
     2881 caaatgtgaa ctgccgccgg aagagcggac caacatcgtc gccaaagtcg tgccgaacga
     2941 agaatttctc ctcgactata tcacgccggg ccggaaattc cgctttgaat tcatccgtta
     3001 gcggatccgg acctggccag gaggatgacc gcccccagga tacaggccat gccggccgcc
     3061 ccggtcaggg tcagggcttc atggaaaata gcaatgccga cgacggtcga cgtcaacggt
     3121 tccactgaag ccaggataga tgcccggccc gcttccatat gcttcaggcc catcgtatag
     3181 aggccaaagg gcagagccgt cgacatcagg ccgagaaggg cgaagaggag gcccgtatag
     3241 ggcacggcgg cggccaggga aaggatcatg ccgaaatggc agaaaggcag gagccctgaa
     3301 gacgcgatga tgaaggtcca ggccgtgacc gtcaggggtt catatttcgc cagggcatag
     3361 cggccaaaaa tggaatagag ggcgtagccg aagccgctgc ccagaccggc caggataccg
     3421 gcagcctcga catgacccat gcctaagccg ctgaccagga cgcagccgac aaaggccagg
     3481 accaggcaga accccttgcg ccgcgtaaag cgttcatgga aaaggaagat agaaaagacc
     3541 atgacgatac tcggcgccgt atagagcagg atagccgcga cggacaaggt cgtcaattcg
     3601 atggctgtga agtagcagat attgaaaaag acgatgctcc ccaggccggt ccccaggaac
     3661 atccagatat cccgcggcgc aatggccaaa ctgcggggct ggcggaatag ggcgacgaca
     3721 gccatggcga tggccgtgac cagggcccgg ccaaaggtaa tctgtaaggg cgacaggcca
     3781 taggccgaca gctggcgggt aaacaggccg atgatgcccc agcagatacc ggctgcgacg
     3841 acgcagaaag gcgccatatg ttccattttc acgcgtatca ccttcttaaa aaaaataggc
     3901 ctggtgaagg ataaacgcct tcgttccagc cttggtttta ttatagcaca gttattatgg
     3961 atgatgatat aataaaaaag taatgtaaac agaggtaatc aaattatgaa tcaagaaatg
     4021 aaactcgccg tcctcatcga cgccgaaaat atttccagca aatacatcga cgtcatctta
     4081 tctgaagcga ataatctcgg cgatgtcatc tacaaacgca tttacggcaa ctggacgacg
     4141 ccccagatgg cgtcgtggcg caataccatt ttagataacg ccatccagcc cgtccagcag
     4201 tacagcaata caatccgcaa gaattcgtcc gactcggccc tcatcatcga cacgatggac
     4261 ttattgtacc agagcaacct cgacggcttc tgcatcgtct cgtcagacag tgatttcacc
     4321 cgcctggctt cgcgcctgcg ggaatcgcag aaatatgtcc tcggtatggg cgaaagcaag
     4381 acgccccggt ccttcatctc ggcctgcaac aagttcctct acctcgacgt cctttgggaa
     4441 gaagccgaag aacacgaagc cggtgaccac gaaggcacgg accatgaaga agcccaggcc
     4501 gaacatacca gtccgcaggc taaggccgaa aagccggaag ccgataatca tgccagcgaa
     4561 acgccggcag cccgtccgct gacaccgccg gaaacgatgc ccctccacgg caagacaccg
     4621 gggaaagatt tctccaccat ccgtcaggcg ctcatcaagc tgaccgaaga aaattccgac
     4681 gacaacggct ggattttctc gggtaccctg ggaaatctcc tgagcaagca gttctccgac
     4741 ttcgacgtcc gcaattttgg ctacaagaaa ttcgttccct tcatcgaatc gttgaaactc
     4801 ttcgacgtca atacctttac agacgaaaag aactgcacag tcaagcacaa ctacttcaag
     4861 ctcaaacaaa cgcccaagcg gccgcggcgc acgttccgcc cctactataa acagcgaggc
     4921 cgttaaaacc actcgattca cggaggatac ccgccatgga tgcgaataca caaaaggcgc
     4981 tggaatcgct gcaccggcga aaggtcaatt cgtccatcgc catcgcagcg gacctggcca
     5041 acgacaactt aaacagtatc tgccgcctgg cgctggcctg gatcagcgac ggcaatgtcc
     5101 acggcatttc ctatttcatc aaaccccggg aaggcccggt ccaggcacac aacctgacgg
     5161 aagccatgct ggccgacagc aagcctttcg atgtcgtctg ggatgaagac attgcctttc
     5221 tcctcaaaga cgccgtcctc tctgcctacc gctcggaacg gctctttccg gccatcaagg
     5281 ccagctatga agcatccggg cgggaactgc ccctgaagga tgtctacatc cgggacttga
     5341 aattcctggc ctcgacgtac atgcccaacc tggccaatga ttcgctgacg tcgatcctgc
     5401 accgcatgaa gatccccgtc gacatggaca atgccctgag ccgggccatg gcctgcatct
     5461 gcggcttgaa ctggctggaa aacctctatc ccatttccag ctacggtatc cccttgtcgg
     5521 ccatcctggc cggtgccctc cagggccagc cggaagaagt cgtcgagacg cgggaagaac
     5581 gggaagcgcg ggaagaaaag gaagccaaat acgccaaact gacgtattac acgaagatgc
     5641 tcttcctgcc cttcgtcatc ttctgcctgt tcatcaccat ctattacgtc caccgctatg
     5701 gagaagccga acagaccaat gtcaacttct cccgctattc agccagcggc gtcccggaac
     5761 tgagcgacga ggaaaaagaa gcgcagcagg aaaagaatcg cctgccctcg aacagcggcc
     5821 gctacctcat gatgcgcggg acctacgtca tccccgacga gacgaccatt cccaccttcc
     5881 tcaaagccag ccacgaccgc gatatggaaa caatccgctc cctcgtccgg tccgacaaga
     5941 tactggtctt tgccaagccg acgcgcatcc agattaccgg tgacaaagac aagaacggct
     6001 tcgtccccgt ccaaatcctg gaaggcgact acgccaatcg cacgggcttc gcctcagacg
     6061 atatgataag taaataaaaa aggggctgtc gcattaagcc atttcggctt tgtgcgacag
     6121 ctctcttttt
//
LOCUS       DAWCYS010000081         6006 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_782_length_6006_cov_6.019997, whole genome shotgun sequence.
ACCESSION   DAWCYS010000081 DAWCYS010000000
VERSION     DAWCYS010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_6006_cov_6.019997"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1305 a         1623 c         1666 g         1412 t
ORIGIN      
        1 tttgggcggc ccctacgcat attatatcat aaacttcatt ggcaaaaggt aaaatatgtg
       61 ctatacttat tttgaatcct tgtaaaggga tattctgtcc atggggatgt aaaggtttcg
      121 acgtggatgg ttgtatcatg aatagcgagc cggggtttca tctgcccgta aatcggtgaa
      181 ccttttaaat taaacgcaaa agaaaacaat tttgctttag ctgcctaatc gcggctaacg
      241 tctcctgctg ctaatcctgc gggcagctcg tagacgtcac ttagcgggat accacatggc
      301 tctgcctggc agtcgtgtcg ggaaactcac aggctggaat cgtctagttt gtccttgtac
      361 gaaaacggtt taagatccta acaaggtctg cgctcggaga agttcatggg gcggcattta
      421 cggacaggag ttcgactctc ctcatctcca ccataggtaa agggcttgtc gcacgacgac
      481 aagcccttta aagttgttag ggactgaccg gaaagaagac aggacagttg ctaaaacgga
      541 gagccttgct ggagctgtca gcgcagctga ctgagaggtt aaacctctcc ggccctttcg
      601 ggccacctct cctatgagga gaggcttgta gataaaaaat ttggaataaa ccattgacaa
      661 attgatgcac aaggtctatg ataagggccg taagttaaac atcgatttta tattttgaca
      721 ttcgatgacg agaaagagta catttttacg gaagcgcaca gcgagccggg tatggtgaaa
      781 gccggcggtg gaaggaaaat ggaaaatcat ctctgagaat tgcagttgaa gtgagtaaac
      841 tgcaccgtct gatccacgtt acaggaaacg cgtatgatag tacgttgaaa agtgccttgc
      901 ttttttttgg cgaggaatat tgggtggtac cgcggatatg aacttccgtc ccttgtggac
      961 gggagttttt tttatttcat gtttatctta tgactatatg actcacaata ggaggaattc
     1021 attatggaaa acatcaattt cgacagtctg aaacgacgga gagatcttcg catcatttta
     1081 ccggtctttc tcgtctcgat tatagcctgc atcgaccgcg tcaatatttc ctacgccaaa
     1141 ctgaccatga cccagaccct gtcctggtta tcgccggaag tatatggttt cggcgccggc
     1201 atcttcttcg ccggctatct gctcctggaa atccccggtt ccctgttcgc cgcgaagttc
     1261 tcggcatcga aatggatttc ccgcatcatg tttacatggg gtatcgtcag cctgtgcatg
     1321 gccttcgtca cgacggaatg gcagttctac ctgtgccgct tcctgttagg cgcatcggaa
     1381 gccagtctct acccggtcat ctattccctc ttgttccccc gctggttcac ggcagctgaa
     1441 cgggcccggg ccacgtcgct catgttgaca tcgcttctcc tttcgacgat catcggcgca
     1501 ccgattgccg gcgtcctcct ggaacattcc ttcttcggcc tcttgggctg gcaggaactg
     1561 tttatcctgg aatccatccc ggctctttgc ttcgctttct atttcttctt cgccgtcaaa
     1621 gaccgtcccg accaggccag ctggctgacg gatgctgaaa aagaatactt gaccaccatg
     1681 taccaggaag aacaggccaa gatgcagagc gtcaagaaat acacagtttt ccaggccttt
     1741 acggatacga aagtcttgaa actctgcctc atctacttcc tctgggtcgt cggtttctgg
     1801 ggcttctact tctggatgcc cacggtcctc aagaacctct ccggctggtc tacgtcgctc
     1861 atcggcgggg ctatcgctat ccccatgatg gcagccctcg tcgtccagct ggtcatcggc
     1921 catacggcta cggttacggg cgataaagta tggcacgtct tcggtgccct ggccgtcggc
     1981 gctgtcggcc tcggcttatc gcctttcgct cataacatgg gcgtcgcctt gttcctcatc
     2041 tgtctgtcgg caatcggcat ccacgcttcc atgggcgtat ggtggaccat cccgacgacg
     2101 ttcctttccg gcccggccgc tgccggctcc atcgccctca tcaactcctg cggcaacctg
     2161 ggcggcatgt tcggtccgaa catgatggcc tgggtccagg ctcagacagg cagcttcgac
     2221 atgggttact acctcatggc cgccttcatg ttctgcgccg gcctcctggt cctgactttg
     2281 aaatataaag tcaacggcgc cgccaaaaaa gacgtcctcc tcgacgccaa agccaacgtc
     2341 cagcctacag tcggacatta gtggctcgtg gctcggaccc acattgtagg ggcgccacgt
     2401 ggggcgcccg ttatgaataa ctggcgccta cgccaccgag ctgtaagggt ccaaaaaaag
     2461 caccccctag gatggcttag gggatgctca cataaaccgt gcgggcgcgc cacgtgcacg
     2521 cccctacagt tcgtaaaaag gcactgtcgc ataaagacag tgccttttta cgtgaatcag
     2581 gcggtccgcc ctttgggcgg cccctacaag ccacgaacca ctagccacta acaccccatc
     2641 gctttcagca cagctccggc caggacggcg tagccttcgg cattgggatg gacgccgtcg
     2701 gcatagagat tgcggcctgt ccgccgttcg gctttgagca gggcttcgta gaagtcgatg
     2761 acgatgcatt ccgtttcttt tccgtaggaa aggagccatt gccgatactg gcggagcccg
     2821 tcgttgtgtt tttctacgtc actggcattt tgccagccgt acatggcact ttccatcttg
     2881 gtcagcggcg ggatgcccag gtatacgggc ttcccctgct ccttgaaagt cgtgacgata
     2941 gtccgcatat tctggcagat tttctccaac tccccgccga ggaggatgtc gttggaaccg
     3001 cccatgatga agaaggcgtc ataccggctg aggtcgaact gatggaggcg atagaccatg
     3061 cccgtcgtcg tatcgccgca ttggccttca ttgtcgatat ggatatggct tttggccgag
     3121 accagggccg tccagctctg gtcgggccgg acgtcgaagc cgtaggtcag gctgtcgccc
     3181 aggcacagga gtttcacggc tggacggctt tctggacggc ctttacggca gcatccgtcg
     3241 acagccacgg ggcatcgaac tggcagccgg ctttttccag gtccatctgg caggcgaaga
     3301 ttttcgggat ggcactggta taaatatcat ggtcatacat atagcgcagc gtcgtttcga
     3361 cgtcggcatc gctgaggatg tggccgtggt tgatgaggac cagccggttg gcatagggca
     3421 gggctgcttc cagcgtatgt tcgatgacga cgatggtatg accatatttg acattcaagt
     3481 cgtggatcaa ttcatagaat tcatgggtcc cttccgggtc cagggccgac gtcggttcat
     3541 cgaagacgag gatatccgga ttggtcgcca gtacgtcggc aatgaccagg cgctgtcgct
     3601 ggccgccgga cagcttcgtg acctggcggt tgcggtacgg cgtcatattg accttttcca
     3661 gggcttccga aatagcctgg ccgatcttat ccggtgcgac gccctgattt tccaggctga
     3721 aggcgatttc ttcttcaacg gtcatggcga cgagctggct gtcataatca tcgagcatgg
     3781 cgccgacacg ggttgccagc tgggaaatcg tcgtctgcgt cgtcgcctgg ccgtcgacgt
     3841 agaccatgcc tttcatgacg ccgccgaaat aatgcgggat agcgccggtc atggccatgc
     3901 acagcgagct cttgccgctg ccgctttcac cggtaatgac caggaagtcg cctttattca
     3961 cggtcatggt gacatcgtcc aggacgggcg tcttctgctt gttatagatg tacgacaaat
     4021 gttccatgga aacgaggcct tcctgggacg gcttcaaatc atacatgctg tggtccgacg
     4081 tgacttcccg ttcttccagg gaatcgagga tgccccggga tacgaaaagg cgcagggccg
     4141 ggaaatacat gagcggtgta acgatggcat tcaaggcgcc gatgacgacg accaagggca
     4201 gcatggccgt aaaatagacg acattcggca gattcatgac gaacttcagg atcgtgacga
     4261 aagcgccgcc actcatgcag gtcgctgcaa agccggtcag gacgggcagg cacgaatgac
     4321 ctttgaattt gatgaatgcc aggttcttga cgatgaacgt acaggtcagg gcgccgagcg
     4381 gttcactgat gaggttgcca taaggaaagc ccgacttcga cgtcaggacg ttgaccaggg
     4441 cagcgaccag gccgatgccc aaggcctgct tgtaactcgg cttggtcagg cagatggcga
     4501 cacaatacgt ggcgatggtc cagttcggcg tgacgccgcc gacgctcggt gagaccaggt
     4561 gcaggatcgt gccgatggcc agcatgagcg tcgtaatggt gatccagcgg aactggccgc
     4621 cctgacgatg atgaaattct aatttagcaa tatctgcaac agttttcatc tttcttccct
     4681 cctaaaccaa gtcttgcagg cgcaagtaaa cgacaaaaca gctcagggcc agcatgatga
     4741 cgatgcccac atagtcgcgg cctttcgggc ggacattgct catgaagctg tgttcgttgc
     4801 tgccgaaacc gcgtaattcc agactgaggg ccatcgtttc cgaccggccc agggacttga
     4861 gcatcagcgg ctggacgaca ctcatataat tgatgacttt cttaaagaca ttgcccttcg
     4921 tcgacagacc ccggcaggcc tgggcttcct ggacggcctt gctttcggcg atgaaatcgg
     4981 gtacgaagcg cagggccgcc gtgaacatga aggcatattc ataaggtatc ttgatctgct
     5041 tgaccagggc ggccgtcaaa tcctgcaggc gcgtcgtcgt cagcaagatg atgaagacga
     5101 tggtcatggc cagcatgcgc aggcccgtga cataagccga ttccagggac ccgccgccca
     5161 ggagctggac gacagccagg aagacggcga agacggccag gctgaagacg gccttgaggt
     5221 tgcgcgacag gctgccgcat aatcccaaga ggatgagttc gatgacgact aagatgacca
     5281 ggggcagcgt cgtatgcatg accagggccc aaaaggaaaa ggcgaaggtg ccgatcaatt
     5341 tcgtaacagg gaccaaattc ttcatcattt tttcatacct ccaatccgtt ccagcatggc
     5401 tgtcgtaaat tctttcatcg atttacaata aggaaaatca ggaaccgcca tggataaagt
     5461 gacggcatcg ggccggctca gacccaggtc atataaatca tcgcgctggg tgaacagttc
     5521 ctgcggcgtc ccgtcgaagg ccttggtatg attgccgatg accaggacgc gctgacagta
     5581 ttcggcgaaa atatccatgt catgactgat gatgaggatg gtcatgccct ttttattgag
     5641 gtccgtcagc aaggccatga gctggtcctt gtcttcactg tcctggccgc tcgtcggttc
     5701 atcgagaacg atatatttcg tatgcatggc gatggctgat gcaatggcga cgcgctgttt
     5761 ttcgccgcgg ttcaagttgg gcggataggc ttcagccagt tccgtcagtt ccgttgcagc
     5821 cagggcttcg tcgacggcct ggcggatctc gtcgcggctc atgcccagct gctgcggccc
     5881 gtaggctact tcttcacgga ccgtcggccg gaacatctgc ccttccggcc gctggaagac
     5941 gtagcccatg aaaatgctgc gcacagaagc ctcgtcatag ttgatgacct tgacgtcatt
     6001 caggat
//
LOCUS       DAWCYS010000082         5976 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_786_length_5976_cov_7.524911, whole genome shotgun sequence.
ACCESSION   DAWCYS010000082 DAWCYS010000000
VERSION     DAWCYS010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_5976_cov_7.524911"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1521 a         1602 c         1557 g         1296 t
ORIGIN      
        1 acctatgaca acgtccgcgc tgccgacgaa tagacagtag aacatcacgt aggacaaaat
       61 aacaaagaag aatataagcg catggccggt gctgtcggcg cgtctgctgc cggggccatc
      121 atcctcggac cagtcggcct ggtcggcggc cttttcgttc atggcaatga agtcgatatc
      181 ccggcaggga cgacgatgta cgccgaaacc aaggaaaatg ccgaagtcgt cggtttgaag
      241 gaaaatggcg tcatggatga catgaagacg gccaatatgg ctgccagtgg cgtacaggtg
      301 ccgacctttg cgcctgtcac caatagtgat gtcgacaaca gcggggctgg cgatgccggt
      361 acgtacaccg acaccgatac ggcgcctcag agcagcaacg gcgatttgca ggccggactg
      421 aaagatggta cggttacgcc tgtcgacctg tacaacagca aacacaatga cgaccagcag
      481 gccgtcgtaa ccattcaatc caatacagca ggtgacgtga atgaataaaa cgtggacagc
      541 ggcgatgatc gcagccctgc tgcctctgac cttttcggcc agtgcagctg atacagcgaa
      601 acttcataca gataaggtca tggccgttcc cgtgacagaa aagaaaatcc tgcccgtcga
      661 tttggaccag gcttctgctt cgcaggacgt cgtcctgccg cagcagcctg tccacatgac
      721 ggctgataca ttcgtcgccc gcaactccga cggctatgtc aagagccggg gcaatgtcga
      781 catcaagcag ggcatggatg aaatccatgc caactatgtc gaaggcaaca tgaagagcgg
      841 cgtctaccat acgaaaggac ccgtagtcta cgtcaacgaa accaatgccc tgacgggcca
      901 ggacgtgacc tataacagcc agaacggcag catgaccatg gataaggtcg aaggcttcgt
      961 cggcccgacg acatacgtcc gcgggaccga tgccgaaatg tacgataata tcggctatat
     1021 caagcatggc ctgattacga cgcctcacgc cgtcgccaag acgccggact attatattac
     1081 cggtgatgac atccgcatct atccgggcga aaagttcacg gcagaaaata cggccctctg
     1141 gttcaagcac atccgcctgt tcacatatgg ccattatgaa ggccgcctgg acagtgaaca
     1201 tacgaaatct tctttattca cgctgctgcc gcggccgacc tacaacagcg atgacggcat
     1261 cggtgtccgg ggctatgccg atgtgcccct caaccagtcc ggcgacctga gcttccatgc
     1321 cagctatgcc ctgaatagca agaatggctt caagccggcg gcccagctcc agaagaatac
     1381 taaaatcggc actttccgtt ttggctacag caaagaagaa agtaccgata acgacaataa
     1441 tatctgggct acgaagtggc cggaactgga atactttgca ccgcgcatcc atctcggcga
     1501 tacgggtatc tatatcgatt ccagtgccaa ctggggccgc tgggctgaag acggcgtcaa
     1561 gacggggacg cataagggct tccgtacgga aatcactcat acgcccattc ccttgtggca
     1621 gcgggccaat gtccgcttct tcgccggcta tcgtaaggat ttatattcta ccaatgatgc
     1681 ccagcgccgc gatccctatt cgggcgtcat cctcaaccag ggcatcaacg accgcctgtg
     1741 gaccagcttc tggtataaga aacacaatgt cagcggctat acgccgtacc gcttcgatac
     1801 cctcgatgac ccgcgccagc atggcatgtc catcggctac gtcctgacgc cgcgggatac
     1861 ggtcatcttc acttgggctc aggacctcga cgatgaccat atttcggacc gcaattacac
     1921 ctgggtccgc gacctccact cgttcattgc taccatcacg tataaacaag tcgataaaga
     1981 atggcaggtc aaaataaagg ccaaggactt cgatttctaa aaggggagag gacaggaaaa
     2041 ctttttgcta tcatgcgcag tccctttctc ttccgcgttt gtaggggtcg ccacgtgggc
     2101 gacccgttag gatttggagg attcttctag caatctgatt gggaatcgta catatcccca
     2161 gagcgggctc gccacgtgcg acaagctctt tttttaccct ctttttacag ggcgtatttg
     2221 catttttgcg ctataatgga ataaagtgag aaaactagat tttacatgaa aggactgtcg
     2281 ctttatgcct ttgatttact gtgtagaaga tgatgaaaat attcgtgaac tcgtcggtta
     2341 tgccctgcgc agccaggact tcgaggtgga aacctttgct gacagcaagg aattctggcc
     2401 ggctttggag aagcggacac cggctttgtt cctcttggat atcatgctgc ccggtgaaag
     2461 cggcaatgat atccttgaca aactgcgcaa gactcagcag tacaagacac tgcccgtcat
     2521 catgctgacg gccaagacca gcgaatacga tatcgtcagg ggcctcgatg gtggcgccga
     2581 tgactacgtc tgcaagccct tcggcatcgt cgagctcatt tcccgcatcc gcgccgtcct
     2641 gcgccgttcg ggccggcaga gtacggaaac gaacctctat acgtatggca ccgtttccct
     2701 ggaccaggaa aaacatatcg taaccgtcga cggcaagccc tgccacttga cggtcaagga
     2761 atttgaactc ctgcgctacc tcctggtcaa tacggatatc gtcctcaagc gggaacagat
     2821 catggaagcc gtttggggct tcacgtatga aggcgaaagc cggaccatcg atatgcacat
     2881 caagagcctg cgccagaaac tgggcgacgg cggcaagatt atccatactg tgcgcggcgt
     2941 gggctatgtc atcggaggga acgtatgaag cggaaagtct acctcagcct gctgaccatg
     3001 gggctggcct gtatggccgt gaccctgctc atttcgacgt ggttcttctg gaactccacg
     3061 cagcaccaga tacagcagga actggtcatg gccatggacg tcgtcgagac gacgatcgag
     3121 gaagggaagg atacgtccgt gtatttgaag aaactcggcg tccacgagac ggatggcctg
     3181 cgcattacct ggatcaatac cgatggcgat gtcctctttg aatcggacta tgacaaaggg
     3241 gtcatggaaa atcacctggc ccggccggaa gtccaagagg ccatcgagaa cggcaagggg
     3301 acggccgtcc gcgattccca gaccgtatcc aaggccctct attattatgc taagaaactg
     3361 cccgacggga caatcctgcg ggtcagtctg gaacgggatg ctttttacgc ccatttcctc
     3421 agcctcctgc cctgggcgct gctgctcctg ggcttgtcgg ccctggcctg catcaaggca
     3481 tcgcggctgc tgacggcgag cctgctcagc ccgctgcggc agacggccct gttcatccgc
     3541 caaatcaacg acagcgatgt gaagatgggg cagccgccgc aggtcgacca tgaactgcag
     3601 ccgttagtcg ataagatttt cttgcagagc cagaccatta ccgacaatat gcgcagcctg
     3661 gagcagcagc gcaatatcat gcgcctcatc atggaaaatc tccaggaagg cgtcatcctg
     3721 acggaccagg cctgcggcat tgccggcctc aacctgcggg ctgcccattt cctcggagcc
     3781 agccagactc aggacgtctt gcagcgcaat ctgaaggaac tcatccctga tgtatcctgg
     3841 gatgccttgc agcatatcga cagtgcctgt gacgtgaagc tgatccggtc ggaccgcctg
     3901 tacttgttga ccattcagcc cgtctataaa gatgacgatt tttatgggac cctcttcatc
     3961 atcgacgacg tgaccgaaca ggaacatcgg gaacagctgc gccgggaatt tacatctaat
     4021 gtatcgcatg aactgaagac gcccctcacg tcgatcagcg gcttcgccga agtcttgtcg
     4081 acgggtattt tcaagaataa agacgacgtc atccatttcg ggacgctcat ccgcaaggaa
     4141 gccctgcgcc tcttgcagat gatcgaagaa atcatgcact tggcccgcat cgaagatggc
     4201 aagcgccaaa tccatcccga ccggctgtgc ctgcgcgatt tgatccagga tatcgtggaa
     4261 ttcatggaac cggtcctgat ggaaaagaaa gttaccgtcc actgcaccat ggacgataca
     4321 gaagtctggg cggaccgcgg cctgttccgg gaaatgatca tgaatctcct cgacaatgcc
     4381 atcaagtaca atcggcctgg cggccatgtc tacatcgatg tccgacacga tgggcacaag
     4441 gccttcttca tcatccgtga tacaggtatc ggcatacctg aggataaaca gcggcgtgtc
     4501 tttgaacgct tttaccgggc tgattccagc cgttcacgaa aaatcaaggg gactggcctg
     4561 ggcctggcta tcgtcaagca catcgtcgaa cagcatcacg gtaccattgc cttgaacagc
     4621 aaagaaaacg aaggcacgac catcaccgtc accctgccga tataggggag tgtatggagg
     4681 tatctaccat catgcaaggt tccttttgct ttatagtttg tagaactgag tttgtagttt
     4741 gcagaagatg ctttaaattt aaaaccattc actacaaact gcaaactaaa aactctaaaa
     4801 agagactgtc ttaggacggt ctcttttttt acaatatttt acaatacata cataaagtcc
     4861 ttacatctcc ttggtaggat aagactgtat tcagaatcag gaacatctca gattcgttta
     4921 tttaaaggag aggatttttt atgaagtgga aaaagttagt tcttgtcgcc ttagctgcta
     4981 ccatggccgt tggggatggc ggtggcggta gtcagaagcg ggctgattcc gggaaaggtg
     5041 ctgctgtttc cggaagcatt actggttccg gttcttcggc tttgctgcct ttggtcaaag
     5101 atgctgccga aaaattcaag gccaacaata aggacgttac gatcaccctt aacgctggcg
     5161 gttctggcac gggtctgaaa caggtttccg atggttctgt cgatatgggt aactctgacg
     5221 taccggctga aacgaaactg gacaaggcca aagcggacaa attggaagac cacaaagtct
     5281 gtgtcatgac agtcgctacg atcattaata aagatgtcgg tgtcaagaac ttgacccgtc
     5341 agcaattaca ggacgtattc acggcgaaag tcaccaactg gaaagatgtc ggcggcaaag
     5401 atatgcctat cgtcctggtt actcgcccga agacgtccgg tacgcgtgcc ctgttcaaac
     5461 agtacgcaat caacggggct gaagaagcag ataacaaatc cctggaaacg gataactccg
     5521 gtatcctcat ccagagtgtc gctcagaacc ccggcgctat cggctatgtc gctctgccgt
     5581 atctgctcaa taacgacacg gttgattccg tttctatcga tggcgtagca ccgacactgg
     5641 aaaataccta taacggtacc tacagcgtat ggggttatga acacatctat accagcaaag
     5701 aaccgaaagc tgctgtcaaa gccttcatcg aatatatcac aggcgctgat tacggcaaac
     5761 gcatcgaaga actcggttat ggcgtaagct cgaagatgca gaccaaagaa gttcactaat
     5821 ttactggagg aacgatatga atgcaatcga aacggtagtc caacgggctc accgtaacga
     5881 gaaagcagcc aaaacgttca tcactatttg cggcatcggt atggcactgg tgccgcttct
     5941 cattggcgct tttctcttca tcaaaggtac agatac
//
LOCUS       DAWCYS010000083         5967 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_787_length_5967_cov_7.862652, whole genome shotgun sequence.
ACCESSION   DAWCYS010000083 DAWCYS010000000
VERSION     DAWCYS010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_5967_cov_7.862652"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1352 a         1832 c         1627 g         1156 t
ORIGIN      
        1 gaagaattgc aggtcacaac gtccatccgc cactgcggct tgtgcaccaa caactgcctg
       61 ctgacggtca acaccttcaa cgacggccgt tccttcatca cgggcaaccg ctgtgaacgc
      121 ggcgccgccg gctcgacagc agcccgcaag aagcctctgc ccaacatgta caaggtcaaa
      181 tataacttgc tcttcggcta taagcccgta gaaaatgcgc cgcgcggcac cgtcggtatc
      241 ccgcgggtcc tcaatatgta cgaagactat cccttctggt tcacgttctt caccaaactc
      301 ggctacaccg tcgtcctctc ggacccgtcg tcgaaggccc tgtttgaaaa ggccatggac
      361 acggtcccgt cggactcggc ctgctacccg gccaaactgg tccacggcca catcgagaac
      421 ctactggaaa agggcgtcga tcgcatcttc tatccgtgca tccagaacgg ccccagcgaa
      481 ggcagcaagg acaatacctt caactgcccc atggtcatga gctatcccga agtcatccgc
      541 aacaacatgg gcgaacgcct ggccgaaacc aatacgccct acctctgccc cttcctgccc
      601 ctccatgaca agaaacgcat cggcaaacgc ctggccgaag aattgaaggc ctggaacctg
      661 ccggctcacg aagtcaaagc cgctgccgct gccgcctggg atgaagaaga agcatataaa
      721 cagaagatat acgacattac caaaaaaatc ctggataacc tggaaaagac gggccagcgg
      781 gccatcgtcc tctccggccg tccgtaccac atcgacccgg aaatcaacca cgtcatcccg
      841 gaactcatca acatcctagg cctggccgtc ctcaccgaag acggcgtcgc tcccctgggc
      901 cagttcccgg gccacctgcg cgtcgtcgac cagtggtcct accattcgcg tatctaccgg
      961 gctgcccagt acatcacaca gcatccgaac ctggaactgg tcgaactcaa ttcctttggc
     1021 tgcggccttg acgccatcgt cgccgaccag gtccaggaca tcctggccca cggccacaag
     1081 atccatacac tgctcaagat cgacgaaggc tccaacctgg gtgccatccg catccgcctg
     1141 cgctcactgc tctttgtcat gaatcaccgt cccaaagaag atcacgtcgt cgaaccgtat
     1201 tcttacgaaa agacgatttt caccaaggaa gacgccaagc agaacgtcat cctggctccg
     1261 tcgatgacac cgattcattt ccccttgttc agcgccgcct ttgaacacga aggctaccgc
     1321 gtcgaactcc tgccccacca gggccagaag gccatcgaca cgggcctggc ctatatccac
     1381 aacgacgcct gctatccggc catcatgagc ctgggccaga tcattacggc cctcaaatcg
     1441 ggcaaatacg acctggacca cacgacagtc atgatttccc agaccggcgg ctgctgccgg
     1501 gcgacgaact acattggcat gatccgcaag gccctgaacg acgccaacat gtcccaggtc
     1561 gccatcttgt cgctgaacct caagggcatg aacaagcagc ccggcttcca gcccggcatc
     1621 ggcttctggc atcgcctggt catgggcatg atctacggcg acgccctgca gcaggtcctc
     1681 taccgcacgc ggccttatga aaaggtcaag gggacagccg aagccctctt ccacaagtgg
     1741 cagaagaaat gcgccgaaca gctgaagaag gccgatttca aatcgttcaa gagaaatatc
     1801 cgcgacctca tcgacgaatt cgaccacatc gacatgagtg aaaccatgaa gccgcgcatc
     1861 ggcctggtcg gtgaaatcta cgtcaagttc caccccatcg ccaataacca catcgtccgc
     1921 atgattgaag aagaaggcgg cgaaatcatc gtctccggcc tggcagactt cttcctctac
     1981 ggccagctgg acaaccagtt ccgccataaa tacctgtcgg gcagctggaa ggattccttc
     2041 ctcagcaaga ctgccgtccg cctcttggaa tggtaccgca agccttacga acaggccgtc
     2101 gccaagagcc gccatttcga cccgatcaca tccatttacg acatcgctga cgaagcgaag
     2161 aaatacttgt ccctgggcca tgaagccggt gaaggctggt tcctcacggg ggatatgatc
     2221 gacctcatcc accgcggcgc caagaacatc gtctgcctcc agccctgggc ctgcctgccg
     2281 aaccacgtta cgggcaaagg catgatcaag accttgaaag ccgccttccc ggaaacgaat
     2341 atcgtcgcta tcgactatga tcccagtgcc agctcggtca atcagacaaa ccgcctgaaa
     2401 ctgatgctga cgacgacttt tgagggcatc cgcgaaccgg tacaggaaga caccaagccg
     2461 gtcatgccct tcatgagcgg tgtccgcatc aacagctcac tggaaaaata gttccaataa
     2521 aagggggtat gtatcatgaa aaacatctta gttcctattg acggttccca gccgtcgcgc
     2581 aacgccttgc aacatgccat cgacattgct aacgcatata gtgccgaatt gaccatcgtc
     2641 aatatcacca atgccgatgc cgtcctggac agcttcgatg tctttgaaga catgaccgat
     2701 gaaaaagtca aggacctgac caaactggcc aaggataaga gcggctacgt cctggaaaaa
     2761 gccatggacg ccgttccgga aaacctgacg gtccacacgg tccagctggt cagtacgccg
     2821 gaaatcggca tcctcagcga agccgacaaa tgcgacgccg acctcatcgt catgggccgc
     2881 cggggcagcc attccctgtc ggaacagctc ctgggcagtg ccagtaccta cgtcctggcc
     2941 catgccaaat gcccggtcct cctggcgcac aataagaaag aaggaccgta ccggcgcatt
     3001 ctcgtcccgg ctgacggctc ggaagaatca ctgaaagccg tccgctacgc cgtagaattc
     3061 gccaaagccg acggcgcatc ggtcacggtc ttccacgtcg ccaacatgcg cgacctcatc
     3121 gccgaagaaa cggcactgga ccagaaaaaa cgcggcctcg aagacttgac cgaccgcctg
     3181 cgccagagcg ccgaccgcgt cttcgccctg tgcaaggctg ccattgccga ccataaaggc
     3241 ggcgtcgaca tccacttcgt cgccaacatc ggccgtccgg gcccgacaat cctcaaaaaa
     3301 gccgaaaaag acggttcgga cctgctcatc atgggcagcc gaggccgcag cggcctgacc
     3361 tcgattctcc tgggcagcgt cagccgctac atcacgaccc attcgggcct gcccgtcatg
     3421 attgtaaaat gatagtcagg tgcggtgcac ctgacgcagg tcgcaggtcg caacggtctt
     3481 atggaccgta cccgcaggga ccgtcctgct aaggcggccc gccctgaatg atggggatga
     3541 cgcagaccgc aacggtccct tggaccgtat gtaggggccg tcctgtcaag gcggcccgcc
     3601 tcgaatgacg gggataatgc actccgacta cacccgaata cataaaaagg ggctatcgca
     3661 ttaagcaaaa acacttaata cgatagccct ttttcattga aatttctgag aaaaagcaac
     3721 ctctcagtca gcattgctga cagctcccct ataaagggag ccagggcagg ccgtaccgga
     3781 cggctcctgc attgcggagt gcggagtgcg acctgcgggc cgccctttgg gcggccccta
     3841 cagttgcata gccctgtaga gtttttcggc ggtcatgggc aggtcgcgca gccagatgcc
     3901 cgtagcgtta tagatggcgt gggcaatggc cggtgccggc gagtcgatga ccagttcgcc
     3961 gatggacttg gcaccgaaag gcccgttcgg ttcatagctc ggttcaaatt cgacgttgat
     4021 ctggcccagg tccatgcgcg tcggcatgcg gtactgcatg aaggagttat tctgcaggcg
     4081 gcccgacggc gtatactgga tattttcggt cagggccatg ccgatggcct gtacgatgcc
     4141 cccttcggtc tggactttgg ccagggccgg attgatgacc gtaccgcagt cgacgacggc
     4201 gtcatactgc accagggaga tgtgccccgt ttccgggtcg acgtcgaccg ttgccgcacc
     4261 ggccatatac ggcggcggac tggtcggcga ataacacgat tctgtcgctt ccagggcgat
     4321 gtcattcatg aacatggcgt cattgcccaa gtccttgagg gtgattttct tgccagtaga
     4381 caagtcagta atggctttgc cgtcgaattc caaatcgtca acgttagctg cgccgagctt
     4441 gacagctgcc cgttctttca tgcgctgtat caggctctgg caggtcttga cgacggcatt
     4501 gcccgtgagg tatgtcgtcg acgacgcata ggatccgctg tcgtacggcg atgtatccgt
     4561 atcgacgcca taggtgacga cattgtcgac agggcacatg agacattccg cggcaatctg
     4621 ggccaggatg gtatcgcagc ccgtccccat atcggtagct ccgatattga gtgaatagaa
     4681 gccgtcatca ttgaccttga tggtgaccga agcgacgtcg acattggaaa tgctcgaccc
     4741 ctgcatggcc gttgcaatgc cgatggcccg gatatggccg ttggacagga cctgacgcgg
     4801 gcagtcatcc ttccagcccg acatctggac gacccgttcc aggcatttat ccagcgtaca
     4861 gctcaaggcc gtttcattga aataggccgg caggacctgc ccctggcgga ccatgttctt
     4921 caggcgcagg gcgacagggc tcatgtgcag aaccgcagcc agttcgttga cggccgtttc
     4981 cagggcgaac agcccctgag tcgcaccgta accgcgataa gcgccggccg caatggtatt
     5041 ggtatagacg caggtatagc tgaagcggaa ggcttcggct gcattgtata aaggaatcga
     5101 cttatagcct gacaaggtga ccgtcgtcgg cgtgtgttcg ccataagcgc cggcattcca
     5161 cagggaatcg aggaaaatgc cgcggatatg gccgtctttg ctggcgccga ggacgacgcg
     5221 gatctgcgat tcatgacgcg gcgtcgatac ggtcatggat tcttcccgac tgtagatcat
     5281 atacgccggc ttgcccgtca gccaggcgat ataagcaggg tagacttcca tgacgacagt
     5341 ctgcttggag ccaaaaccgc cgccgatacg cggcttgatg acgcggatct tcgatttcgg
     5401 gatttccagg gcgttggcga cgacgcggcg gacgtggaac gggacctgcg tcgagctgac
     5461 gatggtcagg cggccgaagg cgtccttata ggcataggcc cggaacgttt ccatagcgcc
     5521 ctgctgattc tgacgcgtat gataggtcct gtcgatgacg acgtccgacg aagccaggac
     5581 ggcatcgacg tcgccgtgtt cgtcacaggc ctgggcgacg agattgcgct tgttgtcgcc
     5641 gccgacatcg acgaggggct tccagttgtc ttcgggatgg acgacgatat cgttgtccag
     5701 agctttggtg aaatcgagga gcggttcctg gacttcgtat tcaacgcgga ggacgcggag
     5761 ggccttgtcg acggcgtctt cattcgtacc ggcgacgagg gcgacgggat cgccgacgaa
     5821 gcggatctgc ttatccagga tgaggcggtc atacgggctc agttccggat aggtctgacc
     5881 ggcaatggtg aaacgcgact tttttatttc tttaggcgtg acgatgcatt cgattcctgg
     5941 ctacttttcg gcagcgctct tattcac
//
LOCUS       DAWCYS010000084         5833 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_804_length_5833_cov_5.613015, whole genome shotgun sequence.
ACCESSION   DAWCYS010000084 DAWCYS010000000
VERSION     DAWCYS010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_804_length_5833_cov_5.613015"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1383 a         1608 c         1588 g         1254 t
ORIGIN      
        1 tgccggcttt aatcatcttt tcgataattt ccggggtatt ggaactgggg cctaaagtgc
       61 agacgatctt tgtttttttc agcatatcta ttctccttgc ttctatttaa tgtaatcgga
      121 tgattctcgt tcagctgacg gcgcggatga ggttgaagac ctgctgggcc gtattggtca
      181 tattgcgcag ggccgtttct ttattcatgg cttcttccag cggcatggcc ttatcgacga
      241 tagaaaagaa agcatcaatg ccctgccggt tgcattcagc agcgtcggcg gtcgtgcagc
      301 ctgccagggc gatgactttg gcaccgtatt gtttagccag tttcgctaca ccaatcggtg
      361 ccttgcccat agccgtctgg gcgtcgaggc ggccttcgcc ggtaatgacg taatcggcat
      421 ccttgacggc atcggccaat cggatggaat cgaggacaat ctggatacct gaacgcaggc
      481 ggccgttcag ataagccagg aaggcgaatc ccataccgcc ggctgcaccg gcaccggctg
      541 tctggctata gtcatgaccg gtataagtct gggtgacggt ggcaaaagac cggctggcat
      601 cgtccaggac tttgacatcc tgcggcgatg cacctttctg gggcccgaag atataggcgg
      661 cgccccaatt tccgtacaac ggattggtca catcacaggc gacatcgaag gtacagtctt
      721 tcagagccgg cagacctggg aatcatcgac agaaacaatg tgccccactt cctgtccgcc
      781 taaaccgaca ggctggccct gggcatctcg gaaaatataa cccagagcct ggagcatacc
      841 gacgccgcag tcactggtcg agctgccgcc caggccgatg atgaaatggc ggatacccgc
      901 ttctatagcc tgcgccatga cctgaccgac gccatatgtc gtcgtgtgca ggggattacg
      961 ctgttcttcc ggcaccaatc ccagcccggc tgcaccagcc atttcaatga cagccgtttc
     1021 catccccggc agatagccgt aaggacagga aacagcttca cctaaaggcc cctggacgcc
     1081 gacatagcgg agctggccgt tcaatccgtc gacgagggct tcgacggttc cttcgccacc
     1141 atcggccaag ggtttgacga tggcctggac aggatggccg ccataggctt tacggacgcc
     1201 ggcctcaatg gcccggccgg cttcgacgga agtcaggcta tttttcaagg aatcaatggc
     1261 tacaacgact ttcatgatga tctctcctct atctgcaaat tcgtaagact gtgtttctct
     1321 atgcctatag tgtagcaaaa gaggtcatcc ctttccatgt tatgagacac aatatattgt
     1381 cattattttt attttctaaa ccaaggaaga gtggcaatgt aaaggggcac ggcatcacag
     1441 aatacccgtg ggtcgaggcc ggtcttccca gcaatccgct tcagccggta ttgcatggta
     1501 ttcttgtgga tgcagagaat ccgtgccgtc tcctggatgg acaaatcctg ctgataatag
     1561 gcctggagta aatggatatc gctgtccgtc aaaaggtgac aaatatcctg acagtagcgg
     1621 cgacgcacgg caccatccag actttgcagc aaaaaggcca atttcatatc atcggcgtgg
     1681 acgcaaaaga cctgccggtc cccggctgcc tgcaaagcca ttttggcaaa acggtacgat
     1741 tcgtagatac ggtggaaagc ttcttccatc ccgataccgg ccaggatttc cggatagaac
     1801 cattctttcc actgaggcag ttttctgtcc cattctgcat agcccctgct gggaacgagg
     1861 acaacgaaag tattcggata aacataagta tataaggaaa cccccaactt ctgacagaag
     1921 ccggccattt tctggcgaaa cgctccagcc tgccggaaac catcatgcca acggaagacg
     1981 acggccgtat agcagcttcc cgccaattca ggctgatctt cagccagctg ctgtaccgta
     2041 tcttcatcgc gataaatcaa ggccatgacc tggcggctgc gatgcgcttc ttcgctgaag
     2101 gcctcctgac tcaggcgtta ttccttgagg aagacttcac aaattttcgt cagcaagaaa
     2161 ccgtactgac cgacgacagc cggattcccg gtaatcccga tgacgcccag gacctgatga
     2221 tccatgatga taggataatt gacccctcgg ctgacgccgt cctcaagccg gtcttcctga
     2281 acagtctgga cctgcccggt ccgggccgca acgtgaccgg ctttatggta cgttccgata
     2341 cgtccgctat ccgtactagc catgatacgc ccatgacggt cgatgaagtt gatattccag
     2401 ccgacaacag ccctggccga atcgacgatt tgctgggcca gttcatccgt aatgcgtact
     2461 ttcacctgaa tacctccctt tcctcctgaa aaaatctcac gacaaaacca cgtccaaagc
     2521 catcatcagg gtaaatccca aggcaaaaga caggacgccg atgtcagagt gttcgccggc
     2581 cgacatttcc gggatgagtt cttcgacgac gacatagcac atggcaccgg cagccaggct
     2641 gaggagatag ggcaggaccg gtacgatgaa ggccgtcatg gcaatggtca gggccgcacc
     2701 gatgggttcg acgatgcccg acaggacgcc gccgagaaag gctttgtgct ttccgacgcc
     2761 ggctgccttg aggggcatgg agatgatggc cccttcgggc agattctgca aggcgatgcc
     2821 cagagacaag gccagggccc cggcggcgct gatgcccgtc tgcccggcta aaagcccggc
     2881 atagacgacg ccgacggcca tgccttcggg gatgttgtgc agcgtcacgg ccaggacgag
     2941 catcgtcgtc cgctgcaagt ggctgtgcgg tccttcagct ttttcactgt acatgtgcag
     3001 atgcggcacc agccggtcca gggccaggag caggaagatg cccagccaga atccggccac
     3061 agccggcaca aaggcgaact tccctaacgg cgccgcttct tccatagccg gtatcaagag
     3121 gctccaaata gaggcagcaa tcatgacgcc cgacgcaaaa ccggtcagcc ctcgttggac
     3181 ctgacgatcc aattcctgtt tcatggcata gacgcaggcc gctcccaaag acgtcccggc
     3241 aaaggggata aggatcccta tgacagcatt ttccataata actttccctc cctgtatctc
     3301 atttagctat atttgatact taatatcgtt ataatttcat tatacacgaa aagaggccgt
     3361 cgtacaaaga cagcctcttt ttttctgcaa actatgaacg cttatattac aaactttcat
     3421 ctaacgcact ttcgccgctg attttcccgg gcaggaccag ggacaggacc aggcagatca
     3481 ggaccggtac gagccagccc aggccgctgt cgcctaaggg caggccctgg atgagggcag
     3541 ccgccccgtc gagatggaag aactgagccg caatgtcgat ggccgatacc aggccggcag
     3601 cggtgacagt acagcgcatg cccagtttcg acaagccgac gaatttattg atgatagtca
     3661 gcatgacgac ggaaatggca atgggataga cgaacaacaa gaggggcagc gtgacctgga
     3721 tgatctggtt caggccgaga ttggccagga ccaggctgac caggcagatg agagtgacgt
     3781 atttcttata cgacaggact gggaactctt cggcaaagaa ggtcgaaacg gcggcgatga
     3841 ggccgacggt cgtcgtgaag caggtcagcg tgaccatgac agccaaaaag gccatgccga
     3901 cagtcccgaa aatctggaat gatgtctggg ataacacata ggcgccgaga ttgacactgg
     3961 cgtcttcata gaccgactgg ggaataggaa aatgattgcc caagagggcc agtcccagat
     4021 agagcccgcc catgaggacg gcgacggcag caccggccat ccagacgctg gcaacgtgtt
     4081 ccttttccga cgcgaagtgg atccgcttca tggcacccag ggcgatgatg caaaaggcaa
     4141 aggaggccag ggtgtcgacg gtgttatagc cttcgacgaa gccgttgccg aaagccgaag
     4201 cggcgtattt ccccatagga gctgctgtag acggcgtcgt ataggcgacc atgccgacga
     4261 tgatgagcag gacgatcatg atagcaaaga taggcgtcaa taccttgccg acgcggtcca
     4321 ggagtttcga cggcgtaatc gacagccacc aggcgcagat gaaatagagc gtcgtgaaga
     4381 tggccagcgg cagcatgcca tcgcccgtga tgggcaggat accaatagaa aaggacgtcg
     4441 ccgccgtccg cgggatggcc atgaggggcc cgatggccag atacatgagc gtcaggatga
     4501 ccagcgaaaa gagcggcgaa atcttatggt tcatttccgc ccggaaaccg ccggggttga
     4561 aggtccccat gatgacggca atgacgggca taccgatgcc ggacaggaga aatcccagga
     4621 cggccggcat gaacgacgaa cctgcctgaa aacctaaaga aggcgggaaa ataaggttcc
     4681 ccgccccgaa aaacatgccg aacaacagca atccgacttg tgcaccattt tttaacatta
     4741 gcagataaca cttcctttac aattttaatt tattcctgta aatctttatt gttacattgt
     4801 aaatttttaa cgggttgctg tcaatatctg ctattataaa aagttaaacg atttatttta
     4861 cgatgacctt ggctacagcc tgagcgacca ggcgttcccc gtccggttcg gcctggagca
     4921 tgttgagata catgcaggac aactgggatt caacataatt cttgaagtaa ttgaacttcg
     4981 caccctggct gtcgccatcg ctcatgccgt actgctggac agctattttc ttgagcaggg
     5041 ccgacgtctt ctggccggct ggtgagctga gtacccagtc atggtcaccg agatgaacga
     5101 tatagccttc ctgactgtgc ggcgatggca ggccattgcc catcttgttg aggacgccat
     5161 aagcgacggg ggctttctga gccatgagag acgcggcttt cagccaagct acgacgtctg
     5221 cttccccttt agcatcattc ggggccccat gggcacaggc ctgagcgacg acgacatccg
     5281 gcagcaggcg gcggtacatc gattcagcga tggaaatgac atgatgtgcc ccctggccgg
     5341 caatcgtata ttccttcagg gatgaaccgt cgggctgcca ctggaagacg aagggtttca
     5401 tgatatcgtg aagcagctgg ccggctaagg ccgtgtccgt gtggacgtca taacagaaga
     5461 cgtcttcata cgtatggatg acttgaattt cgtcttcatg ctggtcctgc ccttcggcgt
     5521 cctcttcaac ctgccactgg tcctcatcgt cctggccaag ctggatttca tcacgtctgc
     5581 catgctggcc aagaaacgga aatacatcat cctcctggcc gtcatcacgc cgacgacaga
     5641 catcctgtcc cagtgcctcc tggccctgcc catgatcgcc ctcctggaaa tcagccgcct
     5701 catcatccgc tatggcctgc atcggtaagg gttcgtggct agtggttcgt ggctagtggc
     5761 tcttggttcg tggctcgtgg tccgtaggag gttgtcttta tagtttgcag tacggagatt
     5821 gtaggggtcg cca
//
LOCUS       DAWCYS010000085         5750 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_818_length_5750_cov_8.330641, whole genome shotgun sequence.
ACCESSION   DAWCYS010000085 DAWCYS010000000
VERSION     DAWCYS010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_818_length_5750_cov_8.330641"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1304 a         1633 c         1548 g         1265 t
ORIGIN      
        1 gcatccgcga aggcagggcc aagggcgtca tgcaaatgct gctcctgttc atcgggggca
       61 cggccagcgc catcatcatc gcctgcggct tcatcctgtc ccgcgtcctg tcccgccaga
      121 tactgacgcc gctggcagcg cttcggacag cggcagctga aatcgagaaa ggcaatctcg
      181 actacgagct ccgcctgtcg tcgcgcgatg acctcggccg gacctgtgaa gccttcgacc
      241 acatgcgccg ccagctgcgc tcagcccgcc tggcccagga aaagtatgag cagaaccgca
      301 aagagctcat cgccggcatc tcccatgatt tgtcgacgcc cctgaccctg ctcaaaggct
      361 atgccagcgg catcctggtc ggcatcgcca agaccgctga aaagcgccat cattacgtcg
      421 agctcatcta ccagaacgcc tgcaccctgg aaaaactcgt cgacaggctg ttcctctttt
      481 ccaaactcga cttgggccaa gtatccttta tgatggaacg ggtatcgctg cgcgattatt
      541 tcgctgattt cgccgccgaa aacatggaac gcctggccga gcggggcctc atcctgcact
      601 attcgccgcc agccggtccg gcctggacag ctatcgacag gatgcagttc cagcgggtca
      661 tcgacaacct attggaaaat gccctgaaat ataaagaagg ccctacagtc accatggacc
      721 tgcacctcgc cgaaacgccg caggccgtcg tcctgaccgc agccgaccac gggccaggtg
      781 tcccggcggc agacctgccc cgcttattcg acagttttta ccggaccgat gccgcccgga
      841 cggatgtcca aaagggcagc ggcctgggcc tggccattgc caaacagatc attacgaccc
      901 tgcacggcca aatccatgca gaagcaacac ccggcggcgg cctgaccgtc gtcatcacct
      961 taccgcggat agaggaggaa tatcatgaaa cggattctga tcattgaaga taacttagaa
     1021 attgccgaac tggaacggga ttttctcgaa gccagtgcca ttgagagcgt catcgaaacg
     1081 gatgggacga aaggcctgga acgggccctg caggaagatt tcgacctcat cctgctggac
     1141 atcatgctgc ccgggaccga cggcttccac atctgccgcc gcatccgcga ggaaaaggaa
     1201 gtacccatcc tgatggtatc ggcaaagcgg gaagacatgg acaagatacg cggcctgggc
     1261 ctcggcgccg acgactacat catcaaaccc ttcagcccga cagagctcgt agcccgcgtc
     1321 aaggctcaca tcacacgcta tgaacggctc atgggcaagg cccagcagaa agcggccgat
     1381 ggcgaagtca tccgcagcgg cgacctggaa atttacgtcg ataagcaccg ggtctacgtc
     1441 aaaggaaaag aaatcgccct gaccaaccgc gagttcgagc tcctcgtctt cctggccaag
     1501 catcccggcc tggtcttcag ccgggaccga ctcttcgagc gcgtctgggg tctcgacgcc
     1561 gaaggcgaca cggcgacagt catggtccac atcaaccgca tccgcgaaaa aatagaaccg
     1621 gacccggcca agcccatcta catcgagacg gtctggggtg ccggataccg gttcaaagaa
     1681 tgacgctatt tacttttctt cccgccgcag gatttcctga ccgactgtca cgccggctgc
     1741 cgaagcctgt accaggccgc gggtgatgcc ggcgccatcg ccgatggcaa agaagttggg
     1801 aatcttcgtt tccaggacat ggctcaattc cagccggttc gagtagaact tggcttccac
     1861 gccgtacaac agggtatggc gcgagttggc accaggtgct acggcatcga gggcttccag
     1921 catttccttg atatcctgca tatgacggta cggcaggacc agcgataagt cccctggcgt
     1981 cgcattgggc atggtcggcc ggaccaggcc ccgctggatg cgttccggcg tcgaccgccg
     2041 cccgtcgagg aagtctccca ggcgctggac gatgacgccg ccgccgagga tattggccag
     2101 gttggcaatg tatttcccgt atttgatcgg ttcatggaaa ggttccgtga atttcttgga
     2161 tacgaggatg gcgaaattcg tgttttcgct cttcttgtga gcataactgt ggccgttgac
     2221 cgtgagcagg ccgtcgttat tctcttcgac gacgaaacca taagggttca tacagaacgt
     2281 gcggatccgg tcgtcaaagg atttgctgaa ataaatgagc ttcgattcat agacgacgtc
     2341 ggtaatcgtt tccagaactt ctgccggcat ttccacgcgg acgccgatat cgacggcgtt
     2401 agacgtcagc ttgaggccca tcttttcgac ttcgtggacg aaccattccg acccttcccg
     2461 gccgggagca ctgaccagat agcggcaggc atagaactgg ccgccgatga ggacgccttt
     2521 gatttcccct ttttcttcga cgatgtgatc gactgccgta tcggccatga tatcgacttt
     2581 cccttccagg aaatcgcgca tattggtcag gatctgggca ttgacatcgg tccccagatg
     2641 gcggatgcgg gccgggatga agcgcaggtc agcagcagcc gctttgcgct tgatatcgcg
     2701 gatacattcc ttgttctcat cgccatagac cttgcggccg gcaccgccaa agcggcagta
     2761 gacgtcgtcg acgtaacgta tcttgtccat gaggtcggcc ttggacatat agtcgtcgag
     2821 attgccgccg tattccgtcg tcagcgtcag tttgccgtca ctgaaggcac cggcgccgcc
     2881 ccagccacag acgacattgc gcatcttatc cttgggcagg gccttcttgt tcaggctcgt
     2941 cgcaatgcgc tcccgtatat cctgtccctt ttcgaccatg aggatcttaa atcctttatc
     3001 ggccagttcc agtgccgtaa aaataccggc cggaccggcc ccgatgataa tgacatcata
     3061 tgtctttact gcatgcataa tcacacatcc tttccaggca gaccctttgc gaacaggagc
     3121 tcacacgtgg caagcgctct aaagtggctc gtggctcgtg gtcgccccta cggcctgcga
     3181 actgcggtct gcgggccgct ctgcaaagcg gcccttaccc cctcacgcta tcatattgtg
     3241 aaaagaaagt caacctatgc tataatgagg taaaacgcta tagaaaggat gatgacttat
     3301 gtctcgtcgt aaccgcccgg ctgtccctga tgactccagc cgggatttaa aacgccagga
     3361 agggattttc ctcagcactt ttgccctcat ggtcctcttt ctcgtatcct tataccttcc
     3421 ccttcccgtg gctgtcccca tcgtactggc cgtcgtcctc gtcgcctgga ctgtcgccat
     3481 gtacgtcaaa ttccatgact tctacaagat gcgcgaccgt ggccagcgga cgtggtgtgt
     3541 caccatttcc atgtacgcca gcctcatcct gaccctggcc tgtgcctggt atttcaccaa
     3601 agatgcccct ctgacggatg aatatgccct ggtcttcctc ttcggcttca tgttcttcac
     3661 gtacatggtc taccgcaccc tgagcccgac tatggtcgtc ggcaaccgcc gcatccggta
     3721 taagtaaaaa agggactgtc gcatgtgcga cggtcccttt ttaagtggct agcagttagg
     3781 ggttagcggt tagccaatga caaacccctt ttgagttgta ggggcgacca cgtggggcgc
     3841 ccgctctgga atatatacgg taggtcgttc atcgttgatt gcataggatg aaaaatatca
     3901 agctatcacg atgaactacg aacgaactac gaaattactg ctgagcacgt tcttttttgt
     3961 agaggtcagc tgtgatggca tcggagcagc atacagcaac gacgtcagca acgtcttcag
     4021 acgagcagcc acgggacaag tcgaggatag gtttatccaa gccctggacc aaggggccaa
     4081 gagccgttgc gttagcgaaa cgctgaacca tcttgtagcc gatgttagca gcatcgaggt
     4141 tcgggaagat gaggacgtta gcgtggccgg caactttgga gcccggtgct ttgtgttcgc
     4201 caacttccgg tacgagagca gcgtcagcct ggagttcgcc gtcgaagtcg aagtcgacgt
     4261 tgcgttcttt caggatttca acagcatggc gtacgttgtc gacggattcg ccagcgccag
     4321 aacctttggt ggagtaggaa aggaaagcga ctttcggatt gaggatctgg accgtgcggc
     4381 gggcgcgttc tacgcagatg caagcgatat cagcgagctg ttccgacgtc gggttgggga
     4441 taacgccgca gtcaccgagt acgaggatac cgttatcgcc atactgcgga gtattggtca
     4501 agagaatgaa agcggaagat acggtcttgt tgccaggacg ggggccgatg acctgaaggc
     4561 ctgcgcggat aacttcagca gaagtcgtag cagcaccgga tacgcagccg tcagcttcac
     4621 cgagagcaac gaggccagct gcgaagaagg tgtagttgtt gagcatgatt tcacgagctt
     4681 tttccagggt catgcctttc ttttcacgac gttttgcgaa gtaagcgcaa agttcgtcca
     4741 tacgatagta agtttcaggg tcgacgactt cgacaccggt gagatcgatc tggaatttag
     4801 cagcatcttt tttgatctgt tccagtttac ctacgagaac gattttagca aaaccatcag
     4861 ccaaaacgcg ttcagcagct ttcaatacac gacggctgtt cgtttccgga aggacgattt
     4921 ttttctgtgc tttggaaacg cgggctttca aatcttctgt aaatctcatt tgggtaccac
     4981 tccttgtaag ttcttagaaa ttagtaggaa tatgtcctta tgctaaagaa aatatcccat
     5041 cgctaccatc cattataata caaaatttat aggaatcaaa catattatta cgcatatata
     5101 aaatgagcag aagaaataga ctatgccgat tgcgcaatcc aaaggccaat gtgatacgta
     5161 ttttatatcg ttcattgcag gggacgccct ttgagcgtcc cgccgcaggc cgcaggtcgc
     5221 aggccgcgga ccgtggcggc aaatcactcg tcatgggtag aacccctcag acgcctgcgg
     5281 cgccagctcc ccttcacagg ggagccatta gccactagcc actaacaacg caaaaagacc
     5341 tgtcacaaag tgacaggtct ttttttcctg gtgggcgata acaggatcga actgctgaca
     5401 tcctgcttgt aaggcaggcg ctctcccagc tgagctaatc gcccaagtgg tgacccctac
     5461 gggatttgaa cccatgttct cgccgtgaaa gggcgatgtc ttagaccgct tgaccaaggg
     5521 gccacgtatg tggaatgtgg tgatccaccc gagattcgaa ctcgggacac cctgattaaa
     5581 agtcaggtgc tctgccaact gagctagtgg atcatgattg gcaggggtag taggattcga
     5641 acctacgaat gacggagtca gagtccgttg ccttaccgct tggcgacacc cctgtatggc
     5701 tcctcaagta ggactcgaac ctacgacaaa tcggttaaca gccgattgct
//
LOCUS       DAWCYS010000086         5707 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_825_length_5707_cov_3.837049, whole genome shotgun sequence.
ACCESSION   DAWCYS010000086 DAWCYS010000000
VERSION     DAWCYS010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_825_length_5707_cov_3.837049"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1298 a         1626 c         1485 g         1298 t
ORIGIN      
        1 cgggcgcgaa agatttggaa acaatccgca aatagcggac ccggccgcca gggtccaggc
       61 ttttcagaga gggcggcggc gttccgtcaa aggtcagttc ttcatatgcc ccgtcttcaa
      121 tgatgggcag gtcatagcgg ctgcataatt ccaggagctg gatgcggcgt cggcggctta
      181 gggtccggcc tgtcggattg tggttcgtcg ggatggtata gaggacgctg ttgcccctat
      241 ctgaagactg gcgcaagtgc ttttccaggg cttccagcga caggccctgg tcgtccatgg
      301 gcaggccgca gaggcgaaga tgggcagact ggaagacctg gagcgatttg acatacgtcg
      361 ggtcttcgac gtaaaccgtc gccccgccgc ggagaagccc tgccgatatg agctggagcc
      421 cctgtagggc gccagacgta atcaggatgg acgacggcgc cacgattgaa ttccgctttc
      481 tggcataccc agccagagcc tgacgcaggg cgggcatccc caacggctcg acatagccca
      541 gggaggtaat ggcatcggcc acatcccgca aagtctggcg gaaggcatcc tgcgggaaca
      601 gcctggggtc cagttcgccc gtaccgagac ggaccaggcg gtcatcgaac tccagggtat
      661 tgatggcctg gacgacttcc tgattggccc ggaaatagcc agccgtcatg taacggttcc
      721 aatccggctg aaccggcagg cgcagggacc aggccgtact ggctacgacc gtcccctggc
      781 tgccccggcc ctggagtacg cccgcttcca atagcggctt cagagcctgg ttcagagtac
      841 tccggttcac ctggaaatgc cgggccatct cgcgctgaga cggcaggcgc gtcccggccg
      901 gccattcacc gctttccatt tttccataca tccattcgag gatttgccgg tatacgggca
      961 cggcctgcgc cgggtcggga tgccattcga tttgcacggg aatatgccgc ttcatcacat
     1021 cgtctccttt ttggttggta taattcacat cgctttggct ggctggaatc cggcatagct
     1081 ctgttatact gtgattatac catacgaacc aactttcttt ggttggaaaa gaaggaggct
     1141 tttttatgtt ctttttccaa ggcctgacca tgggactggc ttacgtcgct cccattggca
     1201 tgcagaatct cttcgtcatc cattcagcgt tatcccattc cctgcgccgc gccgtcgtga
     1261 cagcgctcat cgtcttgttc ttcgacatca gtctgtcgct gagctgtttt tacggcatcg
     1321 gcagcctgct ggatcatctt ccctggctca aagcggtcat tctgggcgcg ggtacgctgc
     1381 tcatcctcta catggcctat ggcatgattc gccaggtacc ggatgcgctg acccttaaag
     1441 gtcagccggc ctctctgggc cagaccgttt ccacagcctg tatcgtcacc tggaggaagc
     1501 ctcaggccat tttagacggc acgatgctcc tcggcgcctt ccgcatcacc ctgcctgccg
     1561 ccgaagccac gaccttcctg accggtgtcc tcctggcatc gggcctgtgg ttcctctcgc
     1621 tcacgggctg cgtcgccgcc ttccgccacg ccatgacacc ccgcatcctg cgcaccatca
     1681 acgtcctctg cggcaccgtc ctcatgctgt acggcctcca tttaggctat accttcgtgc
     1741 agttctttca aatctgagac ggattctcca tgatatgcag gatttctgca tcggtatgag
     1801 ccataccttg attaccaaac tttactgatt tttcaactct ttttcgataa cgggcccgac
     1861 tttagcttcc catgcagcaa aatcttttgt tcccgtcatg acatgccacg atttagcacg
     1921 atataaatgc cggtacaact tctgataatt cgggtccgac gcagccaatt cctgcatgac
     1981 gtcccgacga gccgctaaga gatactgcgg tacgccttct ttcgaagccg gtttcagggc
     2041 ggccatcatg aaaggcttag aagtcaataa ggcaactaat tgtttagccg attcccgctt
     2101 attgcgtgga aggggggaag agatgctgac gtaatcaaca tagtccgtcg ctacatcggg
     2161 atgttcacag ctggaaatgg ccttcacttc tgtataatta ccctgcccga agcggacaat
     2221 ggcactcatc gcttccggat aggcatacaa cgcccggcca tggccctggg caaaccattc
     2281 agcccgcgta aattcattgc catcgtcata acggcccgtc gcagtgcccg tcatcgcgta
     2341 caattccttc aaatactgca tggcatctgc ttgaacgtct cccatggcct gcacgggaac
     2401 gacctttcct gtatggtcct gaagaatatt atagtacttc atttcattat ttaagctgaa
     2461 atcagcgatg acaccttcgt ctcgtccggg aagcaatgcc tgggtcttcc gtggaccgat
     2521 ggcttcataa agctgttgca tcgtctggac acgggatacg gcatgatcct ctttgtggta
     2581 aaacagcgta tccatgcaga ccatctgcgg gatgccgtaa tacgttcgcc gagagtacgc
     2641 ctgttctaac ttccagtctg gataggcaga gcgggctgga atggtttctc ctaagggata
     2701 aagcaaccct ttttctatat attgcggcgc ataaaggcca tcgaagacaa aaacatcact
     2761 tttcggttct tcaaaataga aatcccaatc gacgaaacgc aatgtcacat cgggttcgat
     2821 attcttccat tgcgcagtca aagccgtttc aaaggcctta atatcaggaa catagcggaa
     2881 caatcctaca gtcagcacct ttttgtctgt ctccgttccc tgtcccggtt cactggccat
     2941 ccctgtcacc acagtcatca tggaaaaggc caggcttaaa atgatacgaa ataatcgttg
     3001 tctcatatgc ttatcccctt caacataaat tagccagcag gcaaagccct gccagccaat
     3061 ttacattgta gcatatgtca tcatgcgtag tcacttttac tttatagttt gaagcacaga
     3121 gtttgtagtt tacagagaaa ccttttaaat ttaaaaccat tcgctaactg ctaaccgctg
     3181 actgctaaaa aagcactgtc tccatgcgac agtgcctttt tattactgta cagctcgttt
     3241 ccggctcatc cacaggccga tggcgatgac accggcagct agtacggcct gaagggtcat
     3301 gggtgcccag gcaccggcca tttgctgcat cagggtcccc aagattttat cgtggccgat
     3361 catggaaccg gctgtccagc ccagcaaccc ggcgccggca tagaggatga ccgggaagcg
     3421 gtccatgagc ttgccgatga ccgtactgcc gccgacgatg atggggatgc tgatgaggaa
     3481 gccgcaggcg acgaggagga agctgccgtg cgaagccccg gcgatggcca ggacgttgtc
     3541 gacgcccatg gctgcgtcgg cgatgatgat ggtcttgatg gctcccatga ggctgtccga
     3601 agccgtcacg ttctcatcgt gttcttccgg aatgagcaat ttcacagcaa tgggcagcag
     3661 gatgaggccg cctaaggcct gtaaaaaggg aatcgtcaac agccagacgg cgatgagggt
     3721 catgaccaga cggatgacga cagcgccgcc ggtcccgatg aggatggctt tgcggcgcag
     3781 attggccggc agtttattgg ccgccatggc gatgacgacg gcattatcgc cgccgaggag
     3841 gatatccagt aaaatgatag agcctaaagc catgaaaaat tgcatcgata agatttccat
     3901 aatatacctc tttctctgaa aaacaaaaga gacttccgca tggtatcagc aaaactggta
     3961 tcatgcgaaa gtctcaccca ttgcgatagc aacgctgcca ccagccggtc aatgacggca
     4021 cgatgttgat gacaactgac gagaggttac tccccttcgc gattcgattg taggctaatt
     4081 ataacaggca attcgttctt tgtcaatatc gtttcttccc gtttcatgat ataataaaag
     4141 aaaacacaga ggaggcatca tcatgaatat acaacgtatc ggattcatcg ggaccggtgt
     4201 catgggcagc ggcatgatcc ggaatttaca gaaagccggc tatcccgtca acatcaacac
     4261 gcggacgaaa gccaaggccg aaaagctcat ccaggaaggc tgccgctggt tcgattcgca
     4321 ggcagaatta gccgcccaat cggacgtcgt catgaccatg gtcggttatc cccaagacgt
     4381 ggaagccgtc tatttcggtg accagggcat ctttcgcggc aaacagggcg gtttcgtcat
     4441 cgacatgacc acgtccagtc cttccctggc caagcgcatc tacgcccagg ccaaaaaaca
     4501 gggcatcgaa gccttggatg cacccgtttc cggcggcgac atcggcgccc gtcaagggac
     4561 actggccatc atggccggcg gcgaagaagc ggcctttgaa gccatgaagc ctattttctc
     4621 ggctatgggc cagaccctga cctatttcgg cccggccggc agcggccagt atgccaagat
     4681 gagcaaccaa atcgccatcg cctcgaacat gatgggtgtc tgcgaagcca tggcctacgc
     4741 gaaaaaaagc ggcctcgacc cggaaaaagt catcgccacc ctttccgccg gcgccgccgg
     4801 ctcctggtcc ctggcgaatc tggcaccgcg catgctgcaa ggcgatacga aaccaggttt
     4861 ttacatcaaa cacttcctca aagacatgcg catcgccatc gaatcggcag acgaaatggg
     4921 actggacctg cccggactgc gcttagccaa aaaactctac gaccagctgg ccgaccaagg
     4981 catggaaaac tgcgggaccc aggccttgtt ccagtggtat gccaaacaaa aataagaaaa
     5041 acttagtctt cataccagcc aaacagggtt ttccccgtct ctaactgctg aatccgggcc
     5101 atgtcgtccg gtgtcagggt gacgtcgaaa atatcgagat tctccaccat ccgctcccga
     5161 tgggacgatt tcggaatgac gccgatgccc tggcaccagg tactgcaagg caatctgtgc
     5221 ggccgtcttg ccgtatttct cgccgacagc ctgtaaaatg ggctcctgga atatgggctt
     5281 tttcccttct gtaaaaggga tccatgcctg tatttgtgtc ccctgtgtca caagtgcctg
     5341 ctggagctct tttcgtctgt aatagatgtg gcactcgact tggttgacca tggggataac
     5401 atcagagctt ccgataaaat caagatattc ccgagcattg aaattagaaa tgccaatggc
     5461 ccggatcacg ccctggcggt aagcttcggt caaggcccga tacatatcca aagcctcgtc
     5521 gtaaggctca tggatgagca gcaagtcgat atagtcggtc tgcaaccggg ccagcgattc
     5581 ttcgatgccc tgccaggcca gctcataacc ggcccgggtc tggcacagtt tcgtcgtaat
     5641 gaagacatcc tcccggcgaa ggccgctttc ggccacagcc tggccgacga tggcctcgtt
     5701 atcgtac
//
LOCUS       DAWCYS010000087         5620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_840_length_5620_cov_5.438634, whole genome shotgun sequence.
ACCESSION   DAWCYS010000087 DAWCYS010000000
VERSION     DAWCYS010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_5620_cov_5.438634"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1189 a         1526 c         1409 g         1496 t
ORIGIN      
        1 taattcaagc gggcgttctc gacttgcagg ttcgccagga agttgtccga tacctgcgag
       61 gggttcgcgc caatacctct atacgcatta ccgacggcgg cggtcttgcc gtctgccaag
      121 gtgggggtcg agctggtatc gtacagcggt acattgtccg gcatggatac cgggttatcc
      181 tgtctgaaaa gcatctggtc ggcttccttc tgcttgcggt tcagaatgtt cgcgccgacg
      241 gcgttgccaa taagcatccc cagggcaaag cgcgggtcct gggacgcgta ctgtgcaaag
      301 tccggctgtg gcaacgcctg cgtagtgaac ggcgctgaat actgccgcgg tgcatagttc
      361 tggctgatat tatcttcgat atagttgtat ttcgatttgg ctgccattag ttatcctcct
      421 ttacccagtt gtcggtactg ccgcctttgg cgatgaagcc gtctgcatag tagctgttgt
      481 ccccggaaac gtgcagatca tatactttcc gttccccggc ataaaccatg ttgacgactt
      541 tcccacggcc tttgaggttc gcccctaacg tcatgtcact gatttcaacg aatccgttat
      601 tttccgtgag gagcggctgt gtcaaggtgg ccatgacata gtgggtatct acgccgtctt
      661 tacatacaag gttccataca tcgctataat gcggttccat agtatgcagg accgtttctt
      721 cggattccgt gccgtcttcg tgtggacaaa gtaccttgtc gccaactttg acgtcggtaa
      781 tcggcacttc ggacccatct gccaggcgta ctttcgtttc cggtgcaaaa caaaagatgg
      841 ccccagcatt gctggcaagg ccggtgagga tacccccgaa caggccaccg ccactagtct
      901 tctgcgtcgt ggtcgtcgtg cctttgcctg ccagtgcgcc caatgcgccc gttgtcgagc
      961 cattgaggcc caaagaggcg ttccaaagat tgatagccgg ttgctgtgcg ccttcctgtg
     1021 cggccgccgc tgtggcaatc ggctggcctg cggcgtcgat gttctggcca tagatattcg
     1081 ccagctgaga aacggtgttc tgccagttct gtgccatggc gtcactggcg ctgtcgctga
     1141 tacctttaag gccggtatcc atgacggagc tattgactac cccacgggcg cccatgtcct
     1201 gtaagaggtt gcccatagag ccttgcacgc ccttcttgat actggcttcc atggcgtcct
     1261 ggtaggctgt cggcatctgc ccttgcgcca gtccttcgag accctgctgg ccccatttga
     1321 tttggtccag ggcattgttc atgaggtcgt tataatctac ctgcgtgcta cccaacgagc
     1381 cgtacagcat gttcgcggcg gtatcgttca gtttctccgc gttcggcatg acgtattttg
     1441 agtattccag ggcctgctgt tgcaaagcct tttcttcggc cgacggggta taggtctgag
     1501 acgagctgct ggacttactt ttcttcccca ttcaatcact ccctttcatt cgcatttttc
     1561 cacggcttgt acggccgtct gagttcattg gtaacgtaat aatccatcgt gccgtcgtcg
     1621 ttctctttcg gggtgcagac gacttcacgc ccctgcttat ccttgcagat atagcggtgt
     1681 acgccgttgg taacaaagtc ctgcatgatt ttccagcccc acaaacggat atacggctta
     1741 atgggcagga tgcagatagt aatgatacgg tcatagccga attgcaggcc catacattcc
     1801 agggcgtcgc gccaaaagtg agcgtcgttg caaaggttcc agcaaaggat agccttgctt
     1861 tccggcatgg gcttccactg gcagaatccc cggtctggca gataccacgt agtgaatccc
     1921 gccagggcct ggaaggtatc acctgtcttt tcctcgtata tccttatcca ttcacttaat
     1981 tccttgcttt ccattctatc actcctaaat gtctgcaatt tctagaataa tatggtctac
     2041 cgtgaaccgg tcgttcgact ttacggtaat gtccaggcaa tccgtcgagt ggttgcaccg
     2101 gaacttatta cgatccgccg tggggactgt cacgtccagg gcgccgtcga tgaattccgc
     2161 ctggcctgca taatcggccg tgaacttcgt atcgacgctc tttaggagca tccgttcact
     2221 gctgatagtg gctttcggct taaggatgta ctcgataggc cggccgtcgt catcgagata
     2281 ctgcgaatcc caggcgtaca gcttgccgcc actggctacg atgatagttg acgtcgtttc
     2341 gacgatactg tctatcggca cattgaattt gagtgtggta gcactgccca ggagatagtt
     2401 ataggctaca aaatacttat aatcactcgt cgggcgaatg agtatcatac aatgccgttg
     2461 cagatggtag aaacgcggtt catacatgcc gtccgtcaac aaagcattga atttgtcgcc
     2521 tatatcggac gactggatgt tcccatattc catgacggct gataaggtct ttaggccgcg
     2581 tatggactgg aatatgacgg aactgccgat attgacggca catctcgtgc ctgcaatgtc
     2641 ggtgttgttg gcaatctccg tcacctgcca attagaaggt tctgcatccc ccgacagctg
     2701 atagattttc ccgttggtct tgatgaaaat gatatcggtc gcaaggggga cgatggcagc
     2761 tatatcgccg ctgtcgccgt agccgacgtc taaccactgt tcattatcgg cccggttcgt
     2821 atcttcggac cagtccgtcc cgtcgccgac gccggacagg tagaagccgt ccgagccgtc
     2881 catagatacc gcaatcctag agaaccgctg gaatacgata ttacacgtcg ggctgtcctg
     2941 gactacagtc agctggccgt tctgcgtata gtcgtaatac tggagtttcc cgccactggc
     3001 aatccatacc ttatccatga atttcgcaca aacggggtcg tagctccctt ccaggtcacc
     3061 gatatattcc ggcgtctggc cgacgacgta tttataggcc ttattatggt tcgtgaaaac
     3121 cagtaataag ttggtgtcta cgtcatacca catgtcgcgt acactctcgt tcccgtccat
     3181 ggtataaagc aggcccagcc catcacggcc cgtcaggcgt ttactgtcgc gcgaatagat
     3241 gtaattctgc gctatctgta aatccgacgc gtcgatttgt tccggggcct gcgatacatt
     3301 tacaccaccg acgagggatg agaagacgac ggactgtgtc tgatgtttgt tcagtcttct
     3361 catgttctct ccctacttcg cgatacagcc aatcagaagg acgccggccg ccacggccca
     3421 cgtatcccgt tgccgggtca gcctggcttc cttcttagtc attgagttga tttgctctgt
     3481 caatgtcgct aaggattgat tctgcctgtt caaggcgtcg ctggcttgac tcaatgagct
     3541 gtcggctgtc gtcaatgact gccttgttgt cgtcaactgt tctttcgctt gcgtcaattg
     3601 attctgcaag gctgtcgagt tgctgcccag cctgtctaat ctgctctgta gctgtgctaa
     3661 cgttctctct tgctctctga ttgtcgtctt taactgattg tactgttcta acgacatctg
     3721 cactgtcttc acgggcgcgg ccgtcgtagt atcggcacaa aagccagcca aaggcgaaca
     3781 caaagacaag gacaacacaa gatacaataa gggtcttttt attttctgca atcttatcaa
     3841 cctcctcttt cagctgcgga agatacaaaa tggatcacct cctacaaacg cgacaatgca
     3901 tcatgcattt cactgtcaaa acggttgttc aagctgtccc ggagcgacga gctgttccat
     3961 tccggggtct tgcatacgct gtatacggca acgataaaat catagtcgta ttccggggag
     4021 tcgatatagc tcaaattcgg atagccgtca tagtttcccg tttgtgcgtt gaacatgctg
     4081 tgaacggctt cggtccacat atcgaggata ttcccgacgc cgtactgtac ggcacggctc
     4141 catacgacat cccggagaac gtcgtgatgg ttctcgatat gccagtagtt gtctgccaga
     4201 atctgcacgg ccgggtcgta gtatgccgcc ttgacatact catgctgtgc ctgcgcaaag
     4261 ccgtcactgt cgctggccgc aatattacgc catgcttcat caaagctatc actgcacaac
     4321 ggatacgcgt tgagctgttc cccgtactgc ggatagttgc tattcagcca gctgacgaac
     4381 tgccccagac tgccagcatt ggagctgaac tgatacgtcc cgtaggattt tccgccgggg
     4441 tcgccgtagc cgtcactgat gcagcccggg tcgccgttgc tttcgtactg tgcgcttaat
     4501 tctcctaaca tagtcatcat cctttcgttt tttctgtgct attttccgct gtgggcgtgt
     4561 tttgcggctg agctggcatt gcatatcgcg cggaattgaa gcggctgtca tagccgtatt
     4621 tcgtccagca cgctttccca aggcctacaa cggtagcgat accgccgata acggccgtca
     4681 cgccactcca acagctcatt aattcaaagt gcgtccccct cagtgcgttg ctccagtagc
     4741 cgaaaagcca gctaaagagg acaagaaaca aaaaggccat cataaggata ctcatgatga
     4801 tgattaattg gagccagtgt ttctggcccc actggccaag ggccacgatt gtagttttca
     4861 gttacccacc tgtgattttc tcgatacttt cttcaatatc ttcgatacgg cgcgtgttct
     4921 cctgcgttgt ttcgttgatg cagtgcacat ctttctccag ctcgtgccgg cgttcgcgct
     4981 ccccctggat agtcttgtct aattcagcca aggtatcgtt caccttgagg agcgtttcct
     5041 tgagtggggc cgtgaaggcc ttgcaaatcc atatcatccc gccggccagc gcactgccaa
     5101 cgacaatgaa ttctccaact tctactgtca aaatatcacc accttgttat aatcattcaa
     5161 aaaaatggag tgatacacat gagattaccg agtggatacg gaacctgtta tagactgccg
     5221 gggaatcggc ggaggccgtt tgtcgtgaaa aagaccgtgg atggaagaca aaagatattg
     5281 gggtacttcg atacatttga gcatggaatc gcctaccttt cgtctatcaa taagtcgccg
     5341 ctactggacg acaatattac tttcagtgag ctgttcgccc gttggaaggc tactgagtac
     5401 gacaggctgt ccctgtccag tcgcaagagt tacgataacg cataccgcca ttgccagaag
     5461 ttgcatgaca tgccgttccg tcgtatccgt tacggccatt tgcaggacgt cgtagatgag
     5521 attcaagccg gatattgtac ccagaataaa tgccgcggac tcatgtgcca gctgtacaaa
     5581 tacgccatca aatacgatat tgtcaccacg gactacgcaa
//
LOCUS       DAWCYS010000088         5605 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_844_length_5605_cov_8.243604, whole genome shotgun sequence.
ACCESSION   DAWCYS010000088 DAWCYS010000000
VERSION     DAWCYS010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_844_length_5605_cov_8.243604"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1226 a         1539 c         1568 g         1272 t
ORIGIN      
        1 cgccggcagg gcgctcggcg ccaacgcact ggcggccagc gcgtgcatcg cttctcaggc
       61 cgcggccgct gccgctacgg tcccctgtgt cgctgtcgaa tggattgtcc agggtaaacc
      121 gaccatcctc ggtgccgctt cgggctgcat cgccggcctg gttgccatca caccggcagc
      181 cggcttcgtc gctcccctgc cggccttgat tatcggcgct gtcggcggtg tcatctgcta
      241 cttcgccgtc gccgtcgtca aagccaaact cggttacgac gattccctcg acgccttcgg
      301 tatccacggt atcggcggta catggggctc catcgccacc ggcctctggg ctacgacgga
      361 agtcaacccg gccggggctg acggcctctt ctacggcagc tcggatctgc tcgtcgctca
      421 gattatttcc actgtcgtcg cttacgccct ggccattgtc ggcacgttca tcatcttcaa
      481 gatcatcagc ctcttcatga cggtccgcac cgataagaat gaagaaatca caggcctgga
      541 cattggcgaa cacggcgaac ggggttataa ttatgctatc atgtccggca gccccttcgg
      601 cgaaacgccg gcgacgccgc tcggcaatgc atccttggac aatgccgccg tcaacaacgc
      661 aggcattaac ggtcgtaact tatagaggtg atacgaatgg aagaacatcc gattacaaaa
      721 gtagaaatca tcactcgtcc gagtaaattg gaagagctca aagaagccct gaacaacatc
      781 ggtgtcatgg gcatgactgt cagccaggtc ttcggctgcg gcctccagaa aggctacgtc
      841 gaagtctacc gcggccagcg gtacaacatc aacctcctgc caggcattaa agtcgaaacg
      901 gtcgtctgtg aagtgccggt cgacaaagtc gtcgaagtcg cccagaaagt ctgcaagaca
      961 ggcaaaccgg gcgacggcaa gatcttcatc taccgcctga acgatgccgt ccgcatccgg
     1021 acaggcgaac gaggacctat cgccatcatg gacgaaaagt gagtgtacaa acggggctgc
     1081 cgcatcgcga cagccccttt caagttgtta gtggctagtt gttagttgtt agcggatggg
     1141 tttaaattta aaggtctttt ctacaaacta taaagcacaa ggtgtttcgc atgatgatat
     1201 aaatcttcgt atgacggccc cgctttttgt tattaggggc taacaactta tcatttttgc
     1261 gctataatag cgtcatgaaa gaaaaatcca ctgcaactac tcacaagagg aggaattcaa
     1321 cgtatggtca aaatcggcaa tgttgccctc gacaatggca tgcctaaaat ctgtgtcccc
     1381 cttgtcggtc attctacgaa cgaactcgtc caggaatgcc gctatctcca ggataaaatc
     1441 tacgacgtcg tcgaactgcg catcgacttc ttgaaagacg tcacgtccct cgacgctgtc
     1501 ggcgaagctt tggccgctgt ccgccaggaa ttgccaaacg gcgctatcct gttcacgttc
     1561 cgtactaaag aagaaggcgg cgaaacggaa gttcctgaaa gctactattt cgacctcatc
     1621 ggctacgcta ttaaaagcgg taaagtcgat gccgtcgacg tcgaatactt ccgcgaccgc
     1681 gccagcatcg aaaaagcact ggtcgtcgcg aaagaacacg gcgtaacggt catcatgagc
     1741 aaccacgact tcgacaagac gccgtctttc gatgaaatca ctggccgcct catgggcatg
     1801 aagaaactcg gcgccgatgt ggccaaactg gcctgcatgc cgaactcggc taaagacgtc
     1861 ctgaccctct tgtcggcaac agaagccgtc aaatcccagt atcccgatga accgatcatc
     1921 accatgtcca tgggcaaact cggcgccatc agccgcatca gcggggaaat cttcggctct
     1981 gccctgacct tcggttcggc taaaaaagcc tctgcaccgg gccagctgga agtcacgaca
     2041 ttgcaacaaa tcctgcgggc ccttcactga ggccttcata tatttctgat aagtgcttca
     2101 agggcataga gtacttcttc catgctttta tcattgacaa tgcaataatc agccgcttca
     2161 cggtaaagtg tgatgcgttc ttcgtagagc cggtagactt tctgccggcc ctcttttaat
     2221 aaggggcgga cagctacatc tacatcggag acgatatcat ctggtgaacg gtcgatgaaa
     2281 aatactttgc ccgtcttttt taaaatagcc atattttccg gacgtttgat gacgccgccg
     2341 ccacaggcaa tgataacgcc ctgcttggca gccagcgtac gactggctcg gacttcggta
     2401 tcgcggaagc agtcttcact gacggtaaag agttccggga tggttttacc ttcctgttct
     2461 acgatgacgt cgtcggcgtc gtagaaatca cgtcctaagc gtttcgccaa agctttccct
     2521 aaagtactct taccgcatcc aggcatgccg atgaggacaa tattgacctt atcattcata
     2581 ataagattcc atctccttgg caatttgttc aatcacagac gaatcgatag tccggcccat
     2641 ccagatttct tccgaaccga tggcctgcgc gacgagcatg tacatgccgt tcagggtctt
     2701 ggcgccgtgg cgacgggcgg cctgcaaaat ctgcgtgtct ttcgggttat agacgatatc
     2761 gacgacagcc ggaaaggcgg cgatgatacg ttccgatgca gggcagccgt tgatgtcagg
     2821 gaacatgccg actggcgtgc agttgaccag gacgtcgccg ccctgagaat attcaaagtc
     2881 ctgataatcg atggtatcgg caccgatttt tttggtgaac gcatcgaatt ctgtcttcga
     2941 gtgaggatgg cgggatacga cggtaatcga cttggctccg ctgtcggaca aatactggat
     3001 gatggcccgg gcagcgccac cagaaccgag gacgacgcag tccttctttt ggatgccgat
     3061 accggcatgt tccagggagc gaccgaagcc gctgtagtct gtattatagc caaaaatccc
     3121 gttatctgta aaatggagcg tgttgacagc tccgatgatg cgggcttcgc gggaaatatc
     3181 cgtcaagtac ggcatgacgt cgagtttgta gggaatggtg acattggctc ccgtataccc
     3241 ttcgtcgcgc aggcgctgaa gttcatttgg gatatcgctg cggtccattt ccaggacgcc
     3301 gtactgatgc tgcccttcgg cctgtaaaat cctgaataat ttttcgtgga tagccgggga
     3361 agccgtatga cctaacttag ccccgatgac gcctaatttc atctacattt cctccttggt
     3421 cggatacgta attccctaag attttaaaat aattgcagtg ttctgccaaa ttgcgcagga
     3481 cccgggatgt cgccggattg cgcaggttgc ccatgacgtc gatgtggaaa aagtattcaa
     3541 agggcctgcc cttgaggggc cgcgattcca gatgagtcat gttcatgccg ttatagaaga
     3601 agtagcccag gacgtgatac aaggcgcctg gccggtgctg tgtcgtcagg actagggtaa
     3661 tcttgtcggc ctctggcgac tgttccatat cggcggcgat gatgaagaac cgcgtataat
     3721 tcatggtatt gtcattaata tgttccacca ggacatcgag gccgtacata tcagctgccg
     3781 tcttgttggc aatggcagcg atatgggggt cctccatttt ttgaacctgt tcggcactct
     3841 gagaggtact gaaatagggg tggagctgcc attcgccgtg gtgcttgagg taactgcggc
     3901 actggttcag gccctgcgga tgcgaataga cttcgcggat gtcttcgagt ttcgtccccg
     3961 gcagggtcaa gaggcaatgc tctactttga cgtatttttc gccgacgacg cagcagtcgt
     4021 aacggtggat gaggtcgtat acatcggtga tgccgccagt cgacgaattc tcgatgggca
     4081 ggacgccgta gcgggcctgc cgggtagcaa cggcctgaac gacgtcttca aagtgagagc
     4141 cataagcagg caaaatattc ttgccggcaa aataggactt catggcttca tacgtataag
     4201 aaccgggctg gccgaaacag gcgatgttca agggcatatc ttcagacgca tccattacca
     4261 gcaccgccct ttctccgaca ggcagacgtc gaggatagcc caggcagcgg ccgcttcgac
     4321 gacgggaatg gcgcgctgga cgatgcaggg atcgtggcgg cccttgatga cgaggtcgca
     4381 gttttcctgc gtgtccgtgt cgatggtttc ctgtttctgg ctgattgacg ccgtcggctt
     4441 gacggccagg cggaagacga caggcattcc gttggtgatg ccgccgttga tgccgccgtt
     4501 gtggttggtc cgggtcttga cgacatcgcc atcgtaataa aaggcgtcgt tggcctggga
     4561 gccgcggagg tcagccaggg cgaagccgtc gccgaattcg atgcccttga cggccggtac
     4621 ggagaacatc atgtgactga gctggctttc gacagagtcg aagaacgggt cgccgatgcc
     4681 ggcaggaagg cctgtcacca tacattcgat gacgccgccg acggaatcga ggtcgttctt
     4741 ggcctccagg attttttctt ccatcgggcc ggcctgggcc ttatcgagga cgggcagtgt
     4801 ttcctttttc agcgcctgca gggtttctgc tgtttccccg gcagcatcaa agggcttgtc
     4861 ggcgatgccg gccaggcgca ggatatggga gccgatggta atgccgtggc gggccagtat
     4921 ctgcgaagcg acggcaccgg caaagaccag gggcgccgtc agacggcctg agaagtggcc
     4981 gccgccgcgg taatcgttga aaccgccata gcgtatcttg ccggaataat cggcatggcc
     5041 aggccgcatg atgtggcgca gctggtcgta atcccgcgag tgctggtcac tgttgcggat
     5101 gatagcgcac agcggcgtcc ccgtcgtatg gccgttgaaa aagccgcttt gtacttcaaa
     5161 ggcatctttt tccgagcggg ccgtagacat tttattcttc cccggcgcgc ggcgggccat
     5221 ttcagcccgg acggcgtccc agtcgatggc ctcgcccgac ggcaggccgt cgaggacggc
     5281 gccgatagaa aggccgtgag attcaccgaa aatcgtaagg ccgatcgttc ttccaaaggt
     5341 attcatttta ctccccctcc ttttggatga tgccgcctaa ggcctggaaa tcctgccaga
     5401 attccggata cgatttgcgg acacactcag cgccttcgat gcgcagcggc tggctgcagc
     5461 gctgggatgc gacggccagg gccatggcga tgcgatggtc gttccagccg ttgaccgtac
     5521 ccccggtaaa gccgccgcca ccgtggatga ggaggccttc gtcttcttct tcgatatcgc
     5581 cgccgatttt gttgagctct gtggc
//
LOCUS       DAWCYS010000089         5535 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_852_length_5535_cov_6.583029, whole genome shotgun sequence.
ACCESSION   DAWCYS010000089 DAWCYS010000000
VERSION     DAWCYS010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_852_length_5535_cov_6.583029"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1282 a         1390 c         1526 g         1337 t
ORIGIN      
        1 cgattcggca ggagaggtgg gaaaatagtg gctagtggtt cgtggctcgt gggactgctc
       61 actgctagct gctagtaggg gcgcccacgt ggggcgcccg ttcggaataa tggtatatcc
      121 ctcatacatg acaggtgatg caggaaaaca cgcacatata tcagcatacg ttatgctcac
      181 atggtcatag cgggcttgcc acgtgcaagc ccctacgata tcgcatacag gtgatagtca
      241 ttaacagctg acggctactt cttccacggg cagagttttc gttcggctat atcgagggcc
      301 atgtaggcga tgatgccgat ggcggccagg gtcaggatgg cggcgtacat ttcttcatag
      361 ttgcggactt ccatgcggta caggctggat gcgatgtgcg gccaaaggac gcctgtcgtc
      421 atcagggcat accaggtctg gcagacgcgg tacggttccg gcagcaaggg cttgttcagt
      481 accatgctgg ccgcctgcca taaggcgccg atgatgaaaa aggccagcag gagatgggcc
      541 atatcgatgg gatcacttgt ttttccggac atcttcgatg acacctcctg tcctggcgaa
      601 gacgatggtc ttgtcaccca gggccgctgc ttcttccgga ctgtgggtga cgagaatcgt
      661 cgcggtctga ctttttacga tgtattgttg gaagaggtca tggcattgac ggcgcatgtc
      721 cgggtccagg gctgagaagg gttcatccag gagcagcaac ttcggctgca tggccatggc
      781 ccggcccagg gcggtccgct gtttctggcc gcctgagagt tcccccggat agtgatggcg
      841 gacggctgtc aggcccagcg tgtccaagag ggaggccagc tggtccttat ccaggggaga
      901 accgtggagc ctgcctggaa ggagaatatt ttcttcgacc gtcttccacg ggaaaaggcc
      961 atagtcctgg aagacgatgg ccaggtccgg cctgggagcg gtcatttttt ggcctgcaaa
     1021 ataaatctgc ccttctgtcg gtgcctgcag gccggcgatg aggcgcagca gtggcgattt
     1081 cccgcagccc gacggcccgg agacggtcag gtgctctccc tgggcgaggc tgaatgagat
     1141 gtgctggagc acggcctgga tgcccctgcg gctgtgatag gcgaggccca cgtcgtctaa
     1201 gcgcagcagc ggtccggcca taggctgatt actggacatg ggcgaccatt tcgtcatagc
     1261 tgtaggcctt gggcaacagg cctttttcta ccatccagtt catgatacgg ctgacttctt
     1321 cttctgtcgg cagggcgtgg gccgtataag acggcgtacg gtacgaaccg gcgacggcgg
     1381 ccgggatgtt ggcctttttc atagccatgt cgcgataggc atcgggctgg ctgtccagtt
     1441 tttcgatcgc ttcattgtaa gcgtcgagga aatgggctgc gtcgtcatga tgcttttcaa
     1501 tcatgtcctg agtcaggacg acgacggact gggaatagtt cttttggagt ttcgtatcgt
     1561 cgatgacgac gtgggctccc tgctggacgg cataggctgc cagcgggtcc ggcaggatgg
     1621 cggcctggat atctttaccg gccaggacgg cttccgtgcg cagggccagg tttggcatct
     1681 gaacggtgtg gacttcggcg gccggaatcc ccagttcctg ttcatactgg ttcatcaaat
     1741 agtccatcat ggtattttct gaaatggcga cggaggcacc ggtcagctgc tgcggcgtcg
     1801 taatgctgct gttcggcgac gatacgacga ggaaccggcc ttcctggggg acggcaccga
     1861 gagccatggc gacgacgcgg acgtctgtac cggctttttt cagcaaggcc gtgacgatca
     1921 tgtcggtcat catgccgtcc agttcaccgg cttccatggc tttttgctgg tccgcagcgg
     1981 agctgaattc aatgacctgg acgtcgacac cgtgtttggc aaagagtttt tctgtttctg
     2041 ccgtatagag gggcaggctg tcgtcgatgc gcaggacgcc gattttgtac gtcttcttgt
     2101 cattcgagcc ggacgggctg ccctggcagg ctgtcagcat gagggccgag caggccagca
     2161 cagcggctaa tacaaaacgt aatacacgat tcatgggaaa gacctctttt catagaataa
     2221 aaatgcataa aagtaacacg aaatatgtta tatactcttt attataggag agtcatgata
     2281 taatttcaac cgttttatgc taagatagca aatgggatta actttgattg tccaagaggc
     2341 gttattcctt ttgtattttg aatacatgac agatatatgc aatatgttta ttgtctttcg
     2401 attttgaatg tggtataaat gtatatgtac ataagaaagg ggagttgatt catatggtaa
     2461 aaagtgttgc cgcaccgcag gcaaaaggga agagtgctga agataagatt tttggtgcta
     2521 acaaccgtgc cgtagctttg accgaaaaat tgggggcaga taaagttatt aatgggacag
     2581 tcggttccat gctcgatgaa gatgggaatc tcatcatgct cgacgtcgtc cagaaagcct
     2641 ataaagcctt gacgccgaaa gaaattgtcg cctatgcgcc gatccagggc tatcccgatt
     2701 atttggaagc tgctatcgac cagtgcttcg gtgaatcccg tccggaaggc tacatccgcg
     2761 cctgtgctac gtccggcggt tccggtgtcc tccatcacgt cattcacaac tattccgaat
     2821 ggggcgatga agtcctgacc agtgactggc attggggggc ttacggttcc atgtgcaacg
     2881 ataatggccg caaactccgt gaatttgaac tgctcacgcc cgatggccat ttcaatatgg
     2941 aaagcttcaa agccaacgtc agcgacatgg ttgccaaaca gtacaacacg gttatcatca
     3001 tgaattcgcc ggccaacaac ccgacgggtt acggcctctc cgattcggaa tgggacgaag
     3061 tcctggcctt cttgaaagat gtcgtcaaag gcaaggataa gaacatcatc ttcgtctccg
     3121 acgtcgctta tctcgactac agcggcgaaa aacatgaatg ccgcaaattc ttcaagaaat
     3181 tcggcaacat gccggacaac gtcctcatcg tcgtcgccta cacctgcagt aaaggcttca
     3241 ccatgtatgg ccagcgtatg ggcgccatga tctgcgtcac gccgaacaag gatattgccg
     3301 atgaattcgt cgccatcaat cagtatacca gccgcgctac ctggtcgaac tccaacagcg
     3361 ctgccatgaa ggtcatggcc aatatctgca aggacccggc caaagtcgct gaactcgatg
     3421 cggaacgggc taaatatttc cacctcatcc aggaacgggc tgacttgttc atgaaagaag
     3481 ccgatgaatg tggcttgaaa tatttgcgtt acatttcggg cttcttcatc acaatcccta
     3541 tggaagggtc ccagaaagtc tgcgatgaac tggaaaaaga aaatatcttc ctcgttccct
     3601 tgggcaaagg tatccgcctg gctgtctgct ctgtttccaa gaagaagatc cacggcctgg
     3661 ctgccaagat taaagcgacc ttggatgccg tcggcgctaa acagtaagtc cgtatcggaa
     3721 agggaggctt cctatgttcc agacaatcgg tgtctgcggg acgggcaata tgggccgggc
     3781 catcgtcaaa ggcctggtag ctgcccatgc cattgctgca gaccatatct atctttataa
     3841 tatccacaag gaaaaggccg aagccctggc caaagaaacg ggagctgtcg tcgtcgacac
     3901 gccggaagaa ctggcacgcc agtcccaggg cctgatcatg gccgtcaagc cgaacatcat
     3961 ggaatcggca ctggaagccg tccgggaatc ccttccggcc gatagcgtcc tcatttccat
     4021 cgccgccggc atctctttgg aaaagctggc cttttacacc ggtccggaaa cgaaaatcgt
     4081 ccgggtcatg cccaatacgc cggccatggt cggcgaaggc atggcctccg tatcgcccaa
     4141 tgtcaacgtg actgaagccg aaacggccga tgtcgtcgcc atcttttcca gctttggcaa
     4201 agccgtcgtg accgatgaaa agctcatcga cgccgtctgt ggcctgagcg gcagcggtcc
     4261 ggcttacgtc tatatgttca tcgaagccct ggccgacggc gctgtccgcg aagggatgcc
     4321 ccgccagatg gcctatacct ttgcggccca gaccgtattg ggagcagcca agatggtcct
     4381 ggaaacaggc cgccatcccg gtgccctgaa agatgacgtc tgctcgccag gcggcacgac
     4441 tatcgaagcc gtccggaccc tggaagaaag cagcttccgc gctgccacca tgaacgccgt
     4501 catcgcgtcg gcagaaaaga ataagagttt atagtttgta gtatggagtt tgtagaagag
     4561 ggctttaaat taaaaaaaca tttactaaaa actacaaact gcaaacttaa tactcaaaaa
     4621 gaggctgtct taatgcgaca gcctcttttt ttgcacggtt atgcgattta tacataaaac
     4681 gttaaattac ataaaatcat ttataaaatg ataaatgatt atttacggct tataaactcc
     4741 acattttagt gcagtaatat gcaaaatagt aaacattggt gttcgctata tcgaaaatag
     4801 gtatacgctc atgtccaaat gtaatttaca aatatgacat tgaggtatat aataacagta
     4861 tagagcttgt atccggatgc aatctaagtg agaaatggcg ttattcgtgg ttcgttcatg
     4921 aataagcccc cgcctgttag aggcgtgaaa agtattttat tatgaaaggg tggtatgtaa
     4981 tggttatatt ggccttgctc ccaatcctat tcctaatcgt agcattgggg ttcttgaaga
     5041 tggccggttg gaaagcgtgc ccaatcgcgg ctatcatcgc atttgttgtc gctgtattct
     5101 gttataatat gtcagttccc attgccattt cggcagccct cgaaggcgta gccctggcat
     5161 gctggccgat cttgctggtc atcatcatgg ccctgttcgc ttacaaattg actgtagcca
     5221 caggcagcct ggaagtcatc aaacagatgc tgaccactgt cagtgaagat aaacgtgtcg
     5281 tattcctgct catcgcttgg ggcttcggga cgttcatgga aggcatggca ggttttggta
     5341 ctgctgttgc tatcccggct gctatgctgg ttgccctcgg gtatccgccg atcaagacca
     5401 tcgtcgcctg cttgatttcc aacgccgtag caccgtcctt cgggtccatc ggtatcccga
     5461 cgacgaccat gcctgctgtc accggtttgg atgccggcgt cctggctacg aaagtctcga
     5521 ttgtatcctt tattc
//
LOCUS       DAWCYS010000090         5529 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_853_length_5529_cov_6.297041, whole genome shotgun sequence.
ACCESSION   DAWCYS010000090 DAWCYS010000000
VERSION     DAWCYS010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_853_length_5529_cov_6.297041"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1200 a         1522 c         1477 g         1330 t
ORIGIN      
        1 acctcctctc tccttgctta ctacggcttt atcttaactc acggccaggg gaatgtaaaa
       61 cgcttatatt tcatgtccgg gtattcgttt ttcgtatgac aatgggcgca ggccgcaggt
      121 cgctggatta gggattaggg gttaggggct cgtggctcgt ggctcgtagg ggccgtccca
      181 aagggcggcc cgcctcgtgg ctagtggcta gtggctagta aaggcgcccc tttttttacg
      241 aacaacgaac tacgaacaac gaaaaaaaga gctatcgcct aagcgatagc tcttttttat
      301 gtacaactct tattcagctg ttgtgttgtt gacaacctgt ttgcctttat agtagccgca
      361 agtcgggcat acatgatgag gcatcattgc ttcgtggcac tgcgggcatt ctacgtagcc
      421 cggtgcggtc aatttccagt ttgcgcgacg catcttctga cgagtctggg ataatctgtt
      481 cttcggtact gccatgttct gcacctcctt aaatcacatg gtacgttatt tctagcgctt
      541 ttctttggat cgcaacaatt gagccaggac ttcgagtctt gggtcaatct tgtcggtagg
      601 acaactacag ggtccgtcgt tcaggtttgc accgcactgc ggacagagtc ctttgcagtc
      661 tggccggcac aaaaccctca ttggctcgct gaggatcaac gtttctctaa ttgtctccgt
      721 caaatcgatg tactccccat tatagggcaa tacgtcctcg ggaagctctg cttccgtacc
      781 atagacttca gacagtgttt ccttccgatc caccgatacc ggtgacaggc agcggctgca
      841 ggtgtatttg ccagaggtct gtatggttcc ctgtaccacc atgtggttac catcgaacca
      901 ataagcccca tccacaacta ctgtggcgtc cttccaagga aggtcctcca cttcacctag
      961 ctcggcagcc ggacattcaa aagaaaaggg gaaagacttc ccggcttctt ttagtgcttc
     1021 ttcgacttgt agtttcatat ttctacctcg actatactat tatatctatc tacttgggct
     1081 ttgtcaatca ttttttccgg tctggcccga tgaattcccg caacagcgaa ttgggcggta
     1141 cgacggacga gtcgatgccc ttgaagggct tgaggaaccg caggagccgc cgggcttcgg
     1201 cgtaatggac gctgcccttg tcgatagatt ccaggagcgg ttcggcatag ggcttgagga
     1261 cagacagttc ataaggcaaa gccgtgttga aaatcgtatc ggcatcttcc tgatagggat
     1321 agatgttctt ttcttcgccg cggcggacgt cagcccagat atccatggtc gccatgggcc
     1381 cgcggtcgcg gaactgctgg tcccggacca tgcggcggat gatgcgcgta tccgacgtgg
     1441 aaatgcggtt gtggtcgtta atgcgtatct gcgtcaggac gcccagggaa atcttgacct
     1501 tttcatagcg cggcagcatg taggtcaggg aatcgttcaa ggcgtggagg ccttcgacga
     1561 cgatgggctg gcctttttcc aggactgtcg gcttgtcgtc gaaatggcgg tggcctgtga
     1621 cgaaatcgaa gcgggccagg tggacttcct tgccctggtt gaggtcatcg atctgctggt
     1681 tgaagagcgg tacgtcgata gcccgcaggg actcgaagtc atagctgccg tcgggattct
     1741 tcggcgtatc ttcgcggtta tagaagtaat cgtccaagga aatgccgata ggccgcaggc
     1801 cgttgacgcg gagctgcgtc gtcaggcgct tgcagaacgt cgtcttgcct gccgacgacg
     1861 ggccggcgat gaggacgacc ttgaccttcg gccggcggct gacgatatag tcggccagct
     1921 ggcccagctt cttttcctgc agggcttcgg ccatgtcgac catatcctgt atggtcccgt
     1981 tctcgatgta atgattcaag tcatagacgt attcgcagcg gacgatgcgc ccccattctt
     2041 cagcatccag gaagaggcgg gccatcttgg ggatttcctt gtattccggt aattcattgg
     2101 gattgtcgac ggtcggcgtt tccaggatga cgccgggcgc ataggaccgc aggttgaagc
     2161 aggtcacata gcccatgctg ggcaggagag gccccatata gtagtcaaag gtctggccgc
     2221 actgataggc catgatatgg gtgacgtcga cgtggcgcag gagttccgct tcccgcatca
     2281 tatggcggtt gcggcacatt tccatagccg tttcggcgct ggcgatgacc tgggaaatgg
     2341 gttcatcttg ttcgacgatt tcgtgcatgc gcttcttgat gatgtccacg tcgtggacgg
     2401 tgacggtgtg gccgatttcc agttcgcagt acaaggcctt gcgcagggca tgcttgacca
     2461 ggaccttgcc gtcgggatag aggtctctga cggcgcggac caggaggaag atggccgtgc
     2521 ggatgatgca ctgattgcct tccatggtat tgatgggcag ccagtctacg tcgccgctct
     2581 ggccgacctt ggtctggagg ccgacacatt cgttattgta taaggccgct gcaatgggca
     2641 tgtcgtcgtg ctggcaatac tgttcacaga cctgctgcag cgtcactcct tccttgactt
     2701 cataggaatt ccctgatttc acatcgtgta aagtaatcac aggaatcacg ctccttttct
     2761 ttcggtaata gcatattcat tataccatat ttttgcagcc tgcggcagat gcgccgaaag
     2821 gctgaacgcc taacgcagac tgtggtataa taagaaaaat aggatttcgt gtagtaagga
     2881 aggataatct atgagaaaaa gagtgcttac cttatgggat ggcaaagtct tcctggccct
     2941 ggtcgtcctc atgggctgta tcctagcagg gattgcgtca tatcagcacg tctatatgaa
     3001 agcgcccgat tatgccgtct cccaggtcat ccgtgccgcc cgggacgggg atatggagac
     3061 cttcagccgc cacgtcgatg aaggggccct gagcggccag ttcttcgatg ccctcgtgtc
     3121 ccatacgtcg gacagtacgg atgaattctt tttgctgaat atcatccgct cgccgttgcg
     3181 ggcttctttc atcaccacag ccgacctcta cctgaactgg acgctggccg ggagtacgga
     3241 caatgacgaa tatcagaaga cggccgataa cctgcagcgc accttgaaga gtgccggcct
     3301 ggccgtcccc ctggcgggct ggcacctcga cggcgccgac tggagccatg acaatacagt
     3361 gaagctctac ttgtacaacg accgcctggg cgcgaccgtc ccctgtacgg tcacgctgga
     3421 acagacgccg tcccagacct ggatgatcac gggcctggcc gacccggaag ccttcctcaa
     3481 cgacttgcgc gatgtcatga agaaagaatt ggcagcctac aacaagccgg tccagcaaaa
     3541 aatcgacgcc atcatgactg tccgcgacgt ctcttccaaa atcgtcaccg gccagggccg
     3601 caccttcctg cgcctgagct atacgccggt cttccgcaag gaccgcaatg acatcgcttc
     3661 tgtcaaagcc gtctacaccc tgcgccggga cagcgataaa gccgtcctct atacgtcgcc
     3721 cctgcgcctg tcgacatcgg cggaaaaacg gacccatgag agccagttcc tgctcaatcc
     3781 cctcatcccg tcacagtaca gcctcatccg ttcgcagacc ctggacggca tgaccagcga
     3841 actccacctc acggccatca ccctcaaaga cggcacagaa ttggcattgg ccgacacgtt
     3901 gccggaaaaa taagaaaagg gcctgtcgca tgtgcgacaa gcccttttta gtggctagtg
     3961 gttagctgtt agtggttagc ggatggcttt aaatttactg tagggtcgcc acgtggggcg
     4021 cccgctctgt gatatgtacg attttattac ccaaccagtt tgctagaaga atcgtccaaa
     4081 tccctaacgg gtcgcccacg cggcgacccc tacaaacaaa gcctttatac actaattccc
     4141 gcctgaacag atttcgatga tcttcggttt ggaattttcg gctttcgaca gggaacggtc
     4201 gaagagtttg acgccgacgg cgccgatgag gaagccgatg atgccgccga tgacagccgt
     4261 ctggactgta tcgaagccct gggccagggc caggaaatga ccggcaaagg cgccggcggc
     4321 acagaccaag aggggcatga cgaaacagat gaaagcgccg atgacgatgt tgacatcgcg
     4381 gaattcgaat ttgacgtgct ggccgacttt gacgcctaac gggtcgacag ccgtgacgac
     4441 gatgttttca gccccttcgc aggcgccgca ggcgatgcag tcggaatggc ggccgacctt
     4501 gatttcggcc gtgccttttt cttttataga aataatcgta ccttcttcaa ttttcatagg
     4561 aatcccttct ttctgtgcct attatagcat ggccttttca ttcaatcaat actattttcg
     4621 tgtagtttca aacatagcac catcaggaga ttcccccttt attccatagc gggcgcccca
     4681 cgggtcgccc ctacaatgcg gctttgccgc gtcaaattca tcccatttgt cataaattcc
     4741 ggctgtctac aggattcaca gcgggacgcc ttaacaggac gtcccctacg gtatcgcata
     4801 gaggcgatga taataataag caggccgccg ttcacaggtt caaaccgcaa aaaaacacgg
     4861 cctgcggccg tttcgtaggg gcttccacgt gggaagcccg ctgtgatatt ttgccacagc
     4921 ctagccgagt atgatggcag gtctttacga acaacgagct acgccttttc tcggactcag
     4981 atgcggcttc gccgctataa actcaaacta acaacgacgc ttcggaaaac cctctgcgcg
     5041 tagcgggcgc cccacgggtc gcccctacta gccttatggc atacgcctat tccttcaatc
     5101 attctatttt cgtgtagttt atagtcgtcg taaaaaaagg cgctgtccca tgaagacagc
     5161 gccttttttc tgtaggggcg cccacgtggg gcgcccgctc acgcaaatca tccagttcac
     5221 aagtatctca catataccta caaactccgt actacaagct ttaaagagaa aagagccttg
     5281 catgatggca aaagccttcg tgcaacggcc cttttttagc agtcagctgt tggttttaaa
     5341 tttttagcca cccactacaa acgataacgt catacaaaaa attcacgcgg tccgcccgtt
     5401 gggacggccc ctacgagcca cgagctataa cgttattaca caggattcac gcgggccgcc
     5461 tagcctctgc tgtccgttcc agcaaccatc ttgcgccttc cgttggtcgg cacccgatgg
     5521 gctggaaag
//
LOCUS       DAWCYS010000091         5512 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_856_length_5512_cov_6.118014, whole genome shotgun sequence.
ACCESSION   DAWCYS010000091 DAWCYS010000000
VERSION     DAWCYS010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_5512_cov_6.118014"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1003 a         1489 c         1452 g         1568 t
ORIGIN      
        1 tcaggataca aggtaccgtt cttgatacct tcgttgacct tcgcgacttc cggtgcagcg
       61 gcaatcatgg cgttcatgat gttcttgctg ttattatccg tcgcttcgct gagctgagtc
      121 aagaggtaat tgtcgttata ggccttggca aagatagcgt tgcggattct aaacgccccc
      181 gtcggcgaca aatcgccctt ttcattgaat acggagttgc cttctgcatc gctgaataca
      241 tcacttgcgg cggcccttct gaattcacgg ttcgaggggt tcatgaattc gcccgtgccg
      301 ttatcgacga attgttctaa tgtggacagt ttcagtctat ccgcatcagc cttcgcctgt
      361 tctgcgccgc ccaacttcgc gccgccttcg gtactgttga taatggcgtt ggcatcggcg
      421 tcgtcggccg cctgccgtac caaaacagga tggtccacgc tctgtaccgt ttctggtgca
      481 atcccaagcg agggtgcgat ggaaacgaga tagtccttat aggccttggc actcttcttg
      541 tgtgcgtccg taagtccttt ataggccttc tggacagcca tgacgcggcc gttgccgtta
      601 aggacgacgc cgctgttgtt gataacgggg gcgccttcgt tgacgaactg gctttctgcc
      661 agcagttccg gcttcatgcc cttcgtcatc ttttccacct gcccgcgcat ctgcggacgg
      721 ttgcggtcgc gcggctgata ttctgccgga tagaggtcgt ttacggcgta attcatgtcg
      781 tggctggcag tgatatcacc ggccgggacg atcttatacg tcgcatcgaa tccagcgtca
      841 ttcgtactgg catgtacctt tacggtcctg ccagccggga cccgttccat gagcttctgt
      901 ttctttgcaa ggtagatttt acgcttcttt tgcgtttcaa ggctttcttt ctcctgtaca
      961 ggtgtattta cctgttcggt ctgtttagga gcttctacgg cctgctgagg tacattttga
     1021 ggtatgttct ggtcttgctg tgtcgtaagc ggattaccct tcaaggaatt agcccgttcc
     1081 agttcctgcg tcgtctgtaa atcgtcggcc aatttctgct gttcttgctc cgtgccgtta
     1141 cggaaggtgt tcatgtattc cttgaacttc gggtccttgc ggtcataggt ggataaaata
     1201 cggcctaaac gggcgtgtgg cgtctgttgc tggcttttgg gtacaggtgt agcggacggc
     1261 gctttttcct tgctcatagc gtcgtttacg gccttcgtga tttcatcctt gtaattggcc
     1321 ttgatgaagt cgcggttttc gggcgtatca atgaacttgt cgttgccgtt actatccttc
     1381 gtgaacatgc catcgagggt gttgatttca tcaatgttgt tcgaggtgtc acggatggtc
     1441 tgggcaacgt ccgggacgac gtcgtcctgt acgctctgct ggccttcttc ggcctgcttc
     1501 tgggcattct ctgcgtcctc ttgggcgtcc tgcgccgtct tttcggtgga gtcgtcgttc
     1561 atgatttcat tcatggcctg gtcgctttgg gcttgaatct tgtccatatc gctcttggct
     1621 tcctgcgcgg cctggtctaa ttcacgttca aactgcccgt tatcgctggt gactttaccc
     1681 gatacgccac tagatgcacc gccaggggca gggccgccat ggtcggtaaa gtggacgtta
     1741 cctccgtcag tcgttgcgac aggatgagag aattcgtcca gccctttgag gccgtacttt
     1801 tccccttgct ggacgagcca gtcacgggcg tccgggttct gttccaggaa gtcgtccgag
     1861 aagtcaaagg cgtgtccttc gtcatgatag gaagagccgt caccgttacg tttcaaggac
     1921 gtaactaaca ggcgatggcc gtacttctga tagaagtcgc gggccaggag attcattttc
     1981 gccatggtgt ttggatccat gccgtctacc tgcgggttca tggcttcact taaatcatag
     2041 aacgcttcac tgggagcctc tacattacca ctaccaccgc caccaatatc acccatgtta
     2101 tcatagttgt tcttgactaa ctggcgatac tgttccgctt cttcaccggc cccgtcgtaa
     2161 tcgcggacgc cagcccatac gtcgccgttt tcaccatcta ttttagcctt taggattgcc
     2221 atgcctacca tggcgttctg ataggggtcc gtcttccagt cggggtactg ggaatcgagg
     2281 cccatattgg aaacggtttc ctcctgggcc tgcatgaccc cataaacacc atcatgcggt
     2341 tctggcatgg aaatggcatt gacgtcatcc ccgccgcttt cgcgggcggc aatagccagg
     2401 cctagacgtg gttctacgcc ggaatcatta gcagcctgga taacaatatt ggccatggcg
     2461 ttgccggtat tcggcgacat gcttccgccg ccacttgccg ggcggttctt ggcttcctgt
     2521 tcggccgtgg ctttttcggc cgcaataacg gtatcatacg catgctgtac ctcttccggc
     2581 gcgccgttgc gcaatacccc catgagccag ttaaaattct tttcgccgcc gatttgctca
     2641 accgtattgt tgttgaggaa gttctcgatt tcctgtttct ggtcataggt ttcataatcc
     2701 ttataagctt ccgtagcggc gtcatccgtg ctttcctggg agccttcttg cgattgctgg
     2761 aacgattcaa gcgacgacgc ggcctgcttg atgaggtcgg gattcccgct attagctaat
     2821 tcgacatagg cattatacgc ggcgttgctc atgcctgccg gtttgccgtg gctgagtgta
     2881 tctttgatgc tctgtgcctg ttcctgggat tctgcactaa gtggggcttg ttcggcttcg
     2941 ggatggaatc cggccgctac gttgccaggg acgcccatca aggcgccgcc gacaaaaccg
     3001 ccgcgggccg cttcccaatc ctccttggtc caggtcaagg ggttatacca tccgccatca
     3061 cgcttgcctt ctacgtcgtt ttccagggcg ttctgcgcgc cttcctggta gccttccgta
     3121 aggccgctgg cccctgccag catacccgta cgggccgcac ctttggcgat gctctttgcc
     3181 acaccttccc cggcgctacg gcctaacagt ccggtcgcaa tccccttacc gcctttcatg
     3241 acgcctaatt caagaggtac cgtaaaggta tcgagggcca tgttcttgaa gaacatagga
     3301 atggcccggc gccgtgcttc gtcttcactc ataccgtcct gcatcatgtc gttgacgacg
     3361 gtcccgtatt cagacaggga gtccgccagg gacgagatag gcgaacggac ggtatccgca
     3421 atgagggcct gcccggcttc cgacatggca aggcgtccca acccggcacg tgataaagcc
     3481 gacgttaagg cacgcgtacc acctgcaaca gcggcgcccg gcatgagggc cgacaatgcc
     3541 atgataggca cggtcgagcc gataccggaa ccaacatcgg tagcaaactt attcgggttc
     3601 aggaaatagc tgtcgccgta ctcgttgttc cactggtcgc gataatcctg catcccttct
     3661 tcggcgcgct gtacattcgt caagatacca ttgctaccgc cgacggcgtt ctggataccg
     3721 ccgacgacgt tagccaggcc ttcgtctgcc gcaacgactc cgccgtacag ccgcggccag
     3781 ttagccgccc tgtagtcggc agaatccgtc aagagttcat cgcggacgct ttcgtcttcg
     3841 agttccccga actgtccact ggcaacaggt gtacccggga tgtccgagta cacgctgtcg
     3901 tcgaggggac gaggggtgta attaggtgca atgacagtac ccatgccctg gaaggggtgt
     3961 tcgaggaaat tcttggcggc gcccaatatg ccagacgccg aaaagccaga atcctcagaa
     4021 gaagtatcct gttgcgtatc gttgtcatta cctttgtctt tatatacgcc agcgtcccaa
     4081 gcggccttat caagatagtt gagtgccata tttacctcct attcataatc tgggtgcaaa
     4141 ccataggtag caaaggaaat gtctcctcca ccgtttgcta atatctggtt gatttggtca
     4201 gcccaatccc cacatttttg tttaataata tcttccattt caaacatgct atacttattg
     4261 cccatcttcg cattttgttc aagaaggtcc gtggcattat gccaagcatt ttcttctgaa
     4321 tccggttctt ttagcccttc accgaattta ttattaatga cttgaatagc gtcttgatat
     4381 gcattgtaat acgggctatt cgattcttgg taatcgtcat cgcctttatg gtcgtttacc
     4441 catttattat gcaattcaat gatgctctta gcatcggaaa gtttaatatc tccagaacta
     4501 ccgccggagc tgcttttgcc ggagccgtta ggcccgtatt tactggttat aaacttcgtt
     4561 cggttattat tgtcattttc gcgggctttt tcggtaatag ttacattagt tttccaagta
     4621 tcatgtttta accgtttatc taactgatta tcctcaatct tgccacgcgt tacgatttga
     4681 ccaagggtat atttattttg caagcccata ttggcctctt tatactgctg tgccgtggcc
     4741 gcacggtctt tcgcgtcagc cgttgcgtag tagttcaagc cgttcggcag tgttgccagc
     4801 atctgcgccg acatttgcgg attgtactgg gccatgctct gggcaatcgt ggcggcggta
     4861 tcatactggc cgttcatagc tgcctggtaa tacagcggat acaatgcgct ggtctgatag
     4921 tcgttatact gctgttcttc ggcctgccat accggtctgt accgttcgag cgtggagttc
     4981 acgacgtcaa tcggcatgtc gtgctggatg gcccaatgga caaagtcaga ctcgttcttt
     5041 tgcgcgtcaa tgtgtttcgg gtggccgccg ctcatatccg tttcaaggct ctgctctacc
     5101 tggtcgaacg ggcggatatt ataccgtggc aggttatcca atgcggcctg ccattccttc
     5161 ttcttgtttg cgtccttttc gttgtcatac aaggtattag ccgccatgta ataggggttg
     5221 tcactgcgga gcatataggc cggcgtattg ttaagcttat ccattaaaga ctgtttcttc
     5281 accgggtcgg tttccttgtt gtactggtca agcaggtctt tgtagtacgg gttatcaatc
     5341 tgtgtttcac cagggaagag gcggtccatt aatttctgcg ggtcgttcgg gaatatgccg
     5401 gtatcggtct gtgcggctgt ggtctgggga gcgcttacct gctggcctgg cgtgatcgtc
     5461 agattggcgt gcggggtctg cgtcgcggcg ggcggggtgg tcggcgtagc cg
//
LOCUS       DAWCYS010000092         5399 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_875_length_5399_cov_6.019835, whole genome shotgun sequence.
ACCESSION   DAWCYS010000092 DAWCYS010000000
VERSION     DAWCYS010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_875_length_5399_cov_6.019835"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1090 a         1474 c         1624 g         1211 t
ORIGIN      
        1 gagtcgttgt aatacgtgac gccgtggatg gtccgcactt tttccaggcg gtgttcgacg
       61 cctttgaaag ccttgaggac ctggcggatg acgtcgaggg gcgctccggc agcataggtc
      121 aagagaatcg cagccaggca attctggata ttatgcttgc cgaagaggtg caggtccgtt
      181 tcggcacaga cgacggtatc ctggccatgg aggcgcagga taagctgtcc gtcccgataa
      241 aaggcccctt ccgggacttc gccgcacgtc gagaaataga cgaccgtacc ggtcatctct
      301 ttcgccatgg cccgtacccg ttcgtcatcc taattgagga cagcgtaatc gcccttgtcc
      361 tggttgagga aaatccgttc cttggcagcg gcatagcctt ccatcgtatg atggcggtcc
      421 aggtggtccg gcgtgatgtt gaggattgtc gcggccttgg ccttgatgct gtgcgtgaat
      481 tcgagctgga aactggacag ttcagcgacg acgacggctt cttttggcag gttgtcggcc
      541 tgggccgaca ggccgtagcc gatattgccg ccgacgacgg tctgacggcc tgtggccttc
      601 atgatttcgc cgaccagggt cgtcgtcgtc gtcttgccat tggtcccagt aatgccgatc
      661 atgtcagcat cggtgacccg gcaggcgact tcgacttcac tccagattgg caggccgcgg
      721 cgggccgctt ctttcatgat cgggatttcc ggagaaatga ccggcgacgg gacgacgaca
      781 tccatgccgt ccaaaagagc cgtttcctgg ctgccgaaga ccagctggac gccggcggcc
      841 tttatatccg gccacggctg ttccgccgta tcgatggcct tcatatcatt cagcgtaacg
      901 gacccgccat gacctgccag ggccagagag gcagcgcggc cgctgatgcc tgcgccgatg
      961 actaagaaat gttttccaga aaaatccatg atgctcattg ccaacacctg ctttatataa
     1021 aatatcgtag ttcaatcaga aaagccgcag gccctatttg caggccagta cgacgataat
     1081 ggacaggatc gccatgagag ccgagaagct ccagaacaca gtgacgacct tgacttcact
     1141 ccagccagac agctcaaagt ggtggtggat cgggctcatc ttgaagacgc ggacaccgcg
     1201 ggtcttgaag gaaatgacct ggatgatgac cgacagggct tcgatgacgt acaagccgcc
     1261 agccaggatg aggagcagtt ccgtcttggt caaaatggcc atagctgcca gggcaccgcc
     1321 cagagccaaa gagcccgtat cgcccatgaa catcttggcc ggatgctggt tatagaagag
     1381 gaagcccagg caggccccgg tcagggccgt gccaaagata gcgaaatggg tatagcccag
     1441 ggcgacggcg atgacgacgt aggcaacgga cgtaatggcg caggacccgg cagccaggcc
     1501 gtcgaggccg tcggtcaggt tgatggcatt gctcgtgccg acgatcatga cgaagacgaa
     1561 gagatagtag aacatgccca aatcgacagt gatggaagta aagggaatcc acaaggtcgt
     1621 cgggaaatgg agaaaggcat cgaggatgta gcagaaaatc gcggccatga cgaactggcc
     1681 ggccagtttc tgtttcgccg taagacccag gttgcggtgc ttgatggcct tgatgctgtc
     1741 atcgaggaag ccgatgacgc catgccccaa agccaggaat aaggccaggc ctttgcgcag
     1801 gtccatcgga tcgaagatga ggcacgatac ggtcagcgcc gtcaggatga ggatgccgcc
     1861 catagtcggc gtcccggctt tatatttgtg gctctgcggc ccttcttccc tttcggactg
     1921 gcgggcatgg aattttttca attccttgat gaccagcggt ccgaagacca ggccgatgac
     1981 caggctgacg atgaagctga gaatcagcat tttaaacatt cacattactt cctttcaaaa
     2041 tagggcagta ttttttccat ggcaaagctc cgcgagccct tgaggaggac tgtatcgcca
     2101 tcgtggagga gttctttcag ctggcgcgcc gcctgttcat gcgtatccgt cgtatagacg
     2161 gccgtcatgc ctgcagcttt cgccgattga gcgatatctt tggctaaatc gcccatcgtg
     2221 atgagggcat cgagaccggt ctgggcgacg aggcggccaa tggtttcgtg ttcccgtttc
     2281 gcccagtctc ccagttcgcc catgccgccg aggacggcga tcttgcggcc ctttgtcagc
     2341 aggtccagcg tgcggatggc ttcggccatg gacgcgggat tggcattata cgtatcgttg
     2401 atgaaggtat acggcccgat atgcagcagt tcctggcgca tggccatgcc gtggtacgat
     2461 gccaggccgc gctgtatctc agccgcgctc aggcccgtca gctggccgac ggcaatggct
     2521 gccagggcat tgtagacgtt gtggccgccg atgaccggca cggaaacgtc gaaagactgg
     2581 ccggcgccgc tgcaatggaa gcggacgccc tgctggtcgc agacgatatg gtctgcccgt
     2641 acagccgcct ggtcatcggt cccatagagg acagtcttcc ctttgcacaa agccgtcatc
     2701 gggcggacga agggatcgtc gccgttgagg acagccgtcc ccttttcatc cagggcctgg
     2761 acgagttcgg ctttggcctt ggcgatattt tcctgcgagc ccagcagttc gatatggctc
     2821 ttgccgacgt tggttacgac gccgatagtc ggttcgccga tggcagccag ttcggcaatc
     2881 tgccctaagc cgcgcatacc catttctacg acgcaggctt cgtggtccgg cgtcagctgc
     2941 aagagcgtct tgggcaggcc gatttcgttg ttgaaattct tttccgtctg caagacacgg
     3001 tacttttcgc tgaggacggt agcgatcata ttgcgcgtcg acgtcttgcc gaccgaaccg
     3061 gtgacggcta cgacggggat ggcaaagcgg cggcggtgga agcgggccag gtcctgatag
     3121 gccttgagcg tgtcggcggc gacgaaaatc ggcgccgtca gcccttcata ttccggacgc
     3181 acttccgata cgactgcccc ggcagcgccg gcagccaggg ccttatctac gaaatcatgg
     3241 ccgttgaaag acgtcccttt gaaggcgacg aacaacgaac ccttggcgat ggcccgcgta
     3301 tccgtgcaga catcgctgaa agacacagag gcggccggct tcaggagccg ggcgccagtg
     3361 gcctggatta tttcttcgct agtaaaagca gccattattt atctcctctg tgcagtgcat
     3421 ggacggcgtt gcgcgcttct tcgcggtcat cgaaatgaat ggtcccgtcg ttgaggatct
     3481 gatacgtttc atggcccttg ccggcgatga cgatgatgtc gttggtctgg gccagttcga
     3541 cggcgcggta aatggcgtcg cggcggttga cgatgacttc atggtgctga cccggtttca
     3601 aaccttcctt gacgcccgct tcgacttcgg aaacgatggt ttccgggttt tccgagcggg
     3661 ggttatcaga cgtgacgata gggatatcgg ccagttcggc gccgatgcgg cccatgatgg
     3721 ggcgcttggt cttatcgcgg tcgccgccgc agccaaagac ggcgatgatg cggttcggct
     3781 tcatggcccg cgccgtttcc agagccttct gcaggctgtc cggcgtatgg gaatagtcga
     3841 cgacgatggc gaaatcctga ccggcttcga cgagttcaaa acggcccggt acggagtgaa
     3901 aggtctggat agccttgtcg atagtggctt catcgacgcc ttcggcatgg gctgcgccga
     3961 tggcagccag cgtattgtag acattgaaca agcccgtgat attcatgttg aggtggacgt
     4021 tgacgaaggg gccgacgaca tcaaaggagg atttcttcaa ttcgactttc aagttggacc
     4081 ctttgaggtc ggcattcttt tcgacaccgt aggtcaggac cttgcagtgg caggcctgga
     4141 ggatatcctt ggaccaggga tcgtcgatat tgacgacagc cgtcttgttt tccttgacgt
     4201 ggtcggcagc ggtcaggctc tggaagagct tggccttggc ggcgacgtag ttttccatgg
     4261 tcttgtggaa atcgaggtgg tcctgggtca ggttggtgaa gacagccgta tcgtattcga
     4321 tgccggcgac gcggttcaag gccagggcat ggctggaaac ttccatgacg acatgggtca
     4381 tgcccttttc ctgcatgagg gccaggaagt gctggagttc gacgacatcc ggcgtcgtgt
     4441 tgtggatcgg cagttcttca tcgtcgatga gggcgtggat ggtgccgatg acgccgacat
     4501 gatagcctgc agcccgcagg atatgggcga tgatgtgagt cgtcgtcgtc ttgccgttag
     4561 tgccggtgac gccgatcatg cgcatcttct tgcccggata atcataaaag aacgggacta
     4621 tgtcttccag ggcgtgatgg atgtccggta cctggatctg gatagtgctc ttgggcaggt
     4681 cgaggtgttt cgtcgtcaag acggcgacgg cccctttttc gacagcctgg gctgcaaagg
     4741 cggcgccatc gacgtggaca ccggaaatgc agacgaagag atagcccgct tcaatctggc
     4801 gcgaatccga tgaaataccg gtgatcaccg tatcggcact gccattgaga ttataaatgc
     4861 ccttgatctg tttacaaagt tctcctaccg ttttggtcat aatcataggt cctccttgtg
     4921 acttctttct gtattctcta caagctaaac aacaatgagg tcgtcgcacg aagtgcgtcg
     4981 acctctcggt tttaagcaat tatcgacgtt ttccccttgc ggcctggtcc gtctggctcc
     5041 cgtaaaagat ccgtgacggt gctaccatgc ccagtcgctt cgtcgcggtc gtgtaaattc
     5101 gttccggtgt ttccaatttg gaaatctcga cgcgcagttc atcgttttca cgctgcagct
     5161 gctgcgtctc gcgcttcacc tccatgagtt ctccactctg ctgatacgtc caggatgtca
     5221 gtagattgga cacgatcacg atagccgcaa tcattccaat cggcagcagg acgtaagccg
     5281 ccccccgccg gattgcgtct acggcagccg tccgccataa gggtatgctc ttatcaccat
     5341 gtatgttccc ggctttcctc accagcatac gcaacacatc catttccatg atcatagcc
//
LOCUS       DAWCYS010000093         5392 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_878_length_5392_cov_5.456436, whole genome shotgun sequence.
ACCESSION   DAWCYS010000093 DAWCYS010000000
VERSION     DAWCYS010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_5392_cov_5.456436"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1231 a         1602 c         1503 g         1056 t
ORIGIN      
        1 ccggctgcag cggctcatgg agccgcagaa cgtctatagt ttagcaaaaa ataaagaaat
       61 cgaagaccac gtctatgacg tcatcgttga agggccgacc aagaacgatg ccaactactg
      121 gttcggccgg acgacgggca acaagatgat catctgggaa aatgacggtt ccgtcgccat
      181 cggcgatacc gtgccggtcc aggtcgacaa aggccagacg tgggtcctca aaggtcactt
      241 agtgcactaa aggaaggtga gactttggca ggaaaaaaat taacgcctat gatgcagcag
      301 tacatggaag tcaaagagca gtgccaggat aagattctct ttttccgcct cggcgatttc
      361 tatgaaatgt tttttgacga tgccctgacg gcgtcgcgcg aactcgacct gaccctgacc
      421 ggccgggctg gcggcaataa agagaaggtc cccatgtgcg gcgtaccttt ccattcggcc
      481 gacacctata tcgaacggct ggtccacaaa ggctataaag tcgctatctg tgaacagatg
      541 gaagacccga agctggccaa ggggctggtc aagcgcaaga tcatcaaggt catcactccg
      601 gggacgatta ccctggagaa tgccgtggcg tcgaagaaga acaattacat cggctgcctg
      661 accgaaagcg atgccatcct ttccatcgcc ctcatggacg tcacgacggg cgaatgtttg
      721 tgggccgact gtgcggcgtc ggtcatggaa gattccctgt tcgacatcct ggccgtctac
      781 gaaccgagtg agctcattta cgcccacatg gggaaggata tggatgccgt ccgcgaatac
      841 ctgcgcagcc acctggccca gtgtacggtt acggaatttc ctgccgatga cagtgtcgat
      901 taccgggaac aggcccggtc ctatttcccg ccggaagact ttgccgctgc ctcggtggcc
      961 ggcgagtgta tcggcatgct cctggcctat gtcagtgacg tcatgcagtc cgatatttcc
     1021 cacatcaatt cgctggaaca tatcgataat gaacggcgtc tggtcgtcga tgcggccagc
     1081 ctgcgccacc tggaaatcac gcagaacgtc cgcgacggcg gccgccgggg gacgctcctc
     1141 gatgtcctgg acaagactaa gacggctatg ggcggccggc tcctgcgcaa gtggctggaa
     1201 gcgccgctca tccgcattgc cgacattacg cgtcgccagg atgccgtcga agatttcgtc
     1261 ctccacgaaa tcatgcgcca ggacacggca gacgtgctgg accggattta cgattttgaa
     1321 cgcatcctga cccgcatcga aacggggacc gtgagcccca aggacctggt agccctgcgg
     1381 gaatcgctgg ccgtcatccc gcagctgaaa caggtcctgt ccggtgccag tgccgacctc
     1441 ctgcacagcc tcaatgaccg cttgcagact catgacgacg tctatgacct gctctgccgg
     1501 ggcatcaagg acgaaccggg cctggtcatc cgcaacggcg gcgtcatccg cgacggcttt
     1561 tcgccggaac tcgatgaaat ccgctccatt gcggccaaca gtcatgcctt tttgcaggaa
     1621 ctggaagaga aggaacggga aaagacgggc atcaagatga aaatcggcta tactaaggtc
     1681 tttggctatt acttcgagat ttcccatgcc aataccaagc ccatccccga ttactacgtg
     1741 cgcaagcaga cgttggtcaa tgccgaacgc tacatcacgc cggaattgaa ggaatttgaa
     1801 gtcaaagtcc tgaccagcca ggaacggatg ctggcgctgg aatacaagct cttcggcgat
     1861 atccgccgcc agatccaggg ccatatcccg gccatgcagg aaacggcccg ggccattgcc
     1921 tgcatcgact gcctgtacag cctggcctgc gcggcctatg acaaccgcta cgtccgtccc
     1981 agcctcaata ccaaggcgac gatttccatt aaggacggcc gccatcccat tattgaaaag
     2041 tatttgaaag acgaattgtt cgtgcctaac gacgtcacct tgaaccatga agaccatgaa
     2101 atcctggtca tcaccggccc caacatggcc ggcaagtcga cgtatatgcg ccaggtcgcc
     2161 gtgctggtcc tcatggccca ggccggctcc ttcattcccg ccaaagaagg gtctatctgc
     2221 ccggtggacc gcatttttac ccgtatcggt gccagcgacg acatcctcac cggccagagt
     2281 acgttcatga tggaaatgaa ggaagtgtcg tacatcctga aacacgccac cagccgcagc
     2341 ctgctcatcc tcgatgaaat cggccgcggc acgagtacct tcgacggcat gtccatcgcc
     2401 cgggctgtca tcgaatactg cctgaagcac atccacgccc tgacgctgtt tgcgacgcac
     2461 taccatgagc tcatcgccat ggctgacgat tcgccgaaaa ttaagaatta taccgtcgcc
     2521 gtcaaggaac ggggcaagaa catcaagttc ctgcggcgca tcattcccgg cggggccgac
     2581 cgcagctatg gcctgcacgt cgcccgtctg gccgggctgc ccgaaagcct gctcaagcgg
     2641 gccgacgtca tcttggaaga cttggaaaag aacggcggag cacccgtccc ggccaaggcg
     2701 cctgtgaatc cggcgccgtc acccatggga gacagcctct tttccagtcc cgtcatcgac
     2761 aagttgctca gcgtcgatgt cagcagtatg acgccgattg aagcgatttc tttcttatat
     2821 aatctgcaaa aagatgccaa agaaggaagt gggatccagt gagttcaatc attcatgtct
     2881 tagatgaagc aacgcgcaat aagattgctg ccggcgaagt cgtcgaacgg ccggcatcgt
     2941 gcatcaaaga actcgtagaa aatgccatcg atgccggtgc ccatgccatt gaagtggaaa
     3001 tcgccgacgg cggccagtcc tatatgcggg tcactgacga cggctgcggc atgagtccgg
     3061 aagatgccca taagtgcatc atccgccacg gtacgagcaa gatcagttcc gtcgaagata
     3121 tttttgccat tacgtcttta ggcttccgcg gcgaagccgt gcccagtatc gccgccgtat
     3181 cgcacatgca gatcacgacg cgccaggcca gcgatgactt tgcgacgcac ctcatcctcg
     3241 acgggggcga aatcacggca gaagaccagg ccggcgcacc ggtcgggacg acgatggaag
     3301 tgagcgacct cttttacaat acgccggcgc ggcgcaagtt cctcaagagc gaacggacgg
     3361 aaagttcgaa aatctctgaa atggtgacca aactggccct ggccaatccg gccattgcct
     3421 ttacttttac caataacggc cggacgacaa tgaagaccgg cggcaccggc gatttgcggg
     3481 aaaccgttgc caatatctac ggcgccaacg tcgcccgcga tgtctttgcc gtcactgccg
     3541 accaggacgg tatcagcctg gaaggctatg taggcaagcc gtcggtctta aagagcaacc
     3601 gcaactggca gacgtgcatc gtcaatcacc gcatcgtcca taatcccttg atgttcaagg
     3661 ccattgacaa tgcctatcat gccatgctgc cgaaatcggg ctatcccttt gccatgctcc
     3721 acctccacgt cgacccggcg acgattgacg tcaacgtcca tccggctaag acggaaatca
     3781 aattctctga tgaacaagcc gtctaccgcg ccatctacca cagcatcgtc acggccctgg
     3841 tggcccagga aaagcccgaa gccatcgcca agaccatcgg tgatgctgta ggggccgtcc
     3901 cagagggcgg cccgcagcaa gtccatgtag gggcgcccac gtggggcgcc cgctcgcagg
     3961 agagtggcca ggcaacctcc gtaggggccg tcccaaaggg cggcccgcag caagcccatg
     4021 taggggcgcc cacgtggggc gcccgctcgc aggaaagtgg ccaagcaacc tccgtagggg
     4081 ccgtcccaaa gggcggcccg caatgggaaa aggcaacctc tcagtccgct acgcggacag
     4141 ctcccctatc aggggagcct cttgcggatg gttcttcctt gttttcccag gctttggccc
     4201 agcatggcca gggcggcccg tcggaagcga cgtcggtcgt ttccgaggcg tcagcgccta
     4261 aaatcgtctt tgacggcgat gacgacgtct tcattccctt aggggaagtg gcggactgct
     4321 tcatcattgc caagaagggt caggatttat acatcgtcga tcagcacgcg gctcatgaac
     4381 ggatccgcta cgatacgttc tgcaagcgtg tcgaacggat gccgagccag cagttgctga
     4441 cgccggagtt cgtcgaagtc gacaacgagg atatgacgct cttattggaa cggcaggatg
     4501 tcttcaacga cctgggctat acctattccg aagccgggcc gacgacgctg cgcgtcgaag
     4561 aagtgccctg cgatttgcag accagcgata ttgccgattc tttaaaggat atctgcctgc
     4621 tcctgcatga ccagaaggaa ccggataaag ccatggtccg ccatcgttcg ctggcctatc
     4681 tgtcctgcca cggtgccgtc aaggccggag acagcctcaa tatccgccag atgaagcagc
     4741 tcctggatga tttgttccat acggaaaaac cctacgtctg ccctcatggc cggccgacga
     4801 tcatccgctt tacgccaaaa gaattagcgc acttatttaa acggacatga ctcatatgat
     4861 ttacgtcgtg ccttcattga aggccaaaga aaaattacaa tgtgaaggga aagcctgggc
     4921 cgaaagccag ggctttgcct atgtgccgcg gggaaagcgg accatacagg atttgatgga
     4981 tgaatacggc gaggatttcc tcgtctattc ctcacggggg cctcagatag accgcccgga
     5041 aggcagccat ttcttcagcc tcaacatggc tgaactgcgc atccagaacc tgcggaaagg
     5101 ccagcgcgac catctcctgg aagccctgat gggggtgaag gagtacgacc tgcggcctgc
     5161 agcggcacag ccgcccattt ccgtcctcga ttgtacctgt ggttttggcg ccgatgcggc
     5221 cgtcgcctct ttcggtctgc cggctggctc ccgcgtcgat gccctggaag tatcgccttt
     5281 gctggaagcc gttacggcct ggggcttttc ccactttgtc cataagaaag acgacgtcac
     5341 ggcggcgctg cgccggattt ccctgcgctg tggcgactat cacgattatc tc
//
LOCUS       DAWCYS010000094         5343 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_888_length_5343_cov_10.060325, whole genome shotgun sequence.
ACCESSION   DAWCYS010000094 DAWCYS010000000
VERSION     DAWCYS010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_5343_cov_10.060325"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1183 a         1411 c         1405 g         1344 t
ORIGIN      
        1 acctacgaca aatcggttaa cagccgattg ctctaccaac tgagctattg aggaacaata
       61 gcttcccgaa ggaaatggag cggaaaacga gactcgaact cgcgaccccc accttggcaa
      121 ggtgatgctc taccactgag ctacttccgc atgacgactt tctaagtata tcacatcggg
      181 attgatatgt caacgctgta ttccggcggg ccgttgtgcc cgtcacagtg cttaattata
      241 ataccatgac gtatgagatt tgtcaagaaa aaaaataggc tgtcgcatta aggcagccta
      301 tttttgagta ttaagtttgc agtttgtagt ttttagtgaa tggttttaaa tttaaagcca
      361 ttttctgcaa actacaaact ctgtactaca aactataaag agaaaggagc cttgcatgat
      421 ggcaaatcca ttcgtgtgac aatcccctac gatttacagc ctacgatatt tcccctgcct
      481 gtccgatgaa gccgcggacg acgttgggga tagtcaggtc tttcgttcca gctccgtagg
      541 ccacggtgtc gtagacgaaa tccttcgggc ggacgccggc atgcgccagg actttgacca
      601 gggcagcgcc ggtcggcgtg accagttcgc ctttgatatc cgtcgcatac cagggaatcc
      661 cggccaggag ttcggccgtc gccggtgccg ggacgggcat gatgccgtga gcgcagcgga
      721 caaagccgct gccggcatgg agcggcgacg tggcgatttg ttcgatgccc aggtatttca
      781 ggcaccagac cgtaccgacg atatccagga tacagtcgat ggcgccgact tcatgaaagt
      841 gtatctcgtc gacggggacg ccatgcacct tggcttcggc ttctgccagg gcctggaata
      901 cggccaggct cttggccttg atatcgtcct cgataggtgc accttcgatg atcttggtaa
      961 tttccggaag gccccggtgc tgatggtgat gatgatggcc cgtcatatcg acgagatggc
     1021 cctggccggc caactcttcc ccatgatgcg cgtggtgttc atggtgttcg tgtccctgtt
     1081 catggtcgtg atgctcatga tggtcgtgag ccccgtggtc atgacgttct gcatcgagga
     1141 ggacattata atagatagac tggatgccca gcttgttgac gctgtggcac accaagtcat
     1201 agcccgtcag gttcagcgag gccatggcct gtttaaaggc ttcaaagggg acaccggcct
     1261 ggatgagcat gccgatgaac atattgccgc tgataccgga aaagcaatcg aggtataagg
     1321 ttttcatgga aatcgctcct tattttaaat tttcattcat catctgtacc gatgcgtcga
     1381 gcatggtcag gcccatagcc gagcccatgc cctggtcgag gttctggtcc agttcgacga
     1441 agggcttgag gcccagtttc ttcatctgca tctgatggac cggttccaga tagcgcgccg
     1501 aagcgcagac gtaatcgtca atcgtcgggc ataaggcgcg ggcaatgagg acggcagagc
     1561 cggtgacggc gttgtcaaag atgacgagca gcttgcgctg ggctgccgac aggatgaagc
     1621 cgaccatggc ggcaatgtcc ggagccccgg cacggcgcag gaggtccagg ggatctgacg
     1681 catggaagtg gtgctggtcg atgacttcct tcaatttctc taatttctga ccggacttca
     1741 tgtgctccgg cagttcttcc agctggtccc ggaaaaagac agctgtcaat atgaaagccg
     1801 tcagcagtcc ccgttcgccg aggttcccca gacctacagc cgtatagcct tcatcggcca
     1861 ggttctgtcc gatctgcagg ccgctgaaga gggcatccat catttcatct tcgctcatgg
     1921 catcctggcg ggcccagaaa tggctgccgt gcatgacttt taaattctgt acgccctgcg
     1981 attccgggat atcttcttcc aggcccatgt caaggagcat gacgccggca ccgatgcggt
     2041 gggccacctt attgataggc cctcggccag tggcgatttc cagggcgtcg gccttgctgt
     2101 tcttgccctt ggtcttattt tctccgccat cgacggcatg gtcagctgca aagacgacga
     2161 cggctttctg caatttttcc ggatgcagcg tcccctgtac accggcaatg cggcctgcca
     2221 gataggacaa cttgctgtcc tggcgtatgg tcagactggc caggcgctga tagcactctg
     2281 ccgtttttgc caaatccatc ggttcgatgg cactgatgac ttgttctaat acttggtcca
     2341 taatcccctc cgtggcgtga aaagtttgaa aaggagctgt cgcaataaga cagctccttt
     2401 ttgagtttta agtttgaagt tttcagtttg tagtatatgg taaaaattta aaaacatctt
     2461 ctacaaacta caaactccat actacaaact aatcttatta taaaattcat tatacacgaa
     2521 aaagccagtt atcgctacat catgacgttc atcgtagtcc gttcatcgtt catcgaagca
     2581 cttcgaccaa cggccttcaa ggtattataa cgaacggccg aattcgtcgg tatccatgat
     2641 ggcttttttg aaagcttctt tggtcagcgg ttcggggacg caggtccatt cggtcagggc
     2701 cggagcagca tcaacgatgg aatcgacttc gccggctttg atatcgaggt cagccagggt
     2761 cgtcgggtaa tggcacttct tatagagggc cgctacctgg tcgcgcagtt caaactgttt
     2821 gtcataggtc agcaggacca ggacgccgaa ggaaaccgtt tcgccatgga gatggccttc
     2881 gacgacttgc ggcaggaccg tcgacgcgtt atagaacatg tgagccaggg acgaattgta
     2941 gtagtagtcg ccgccgcccg tcgtacagtt ggaaacgagg cccgtgctga tgatgatatc
     3001 caggacgacc tgctgcaggg cataggtgat cttcttggcc ttgaaatctt ccagggcctg
     3061 ttcaccatac tgcaagagcg gcgccgtgca ggctttgccg atggcccggc ccaggaggac
     3121 cgtatggaag tgttccgccg tacggctggc caattccgat tcacattctt tactcatagc
     3181 atcgccgatg ccggcccaga agaacttgaa aggcgagtcg gcaatgatgg ccatattgat
     3241 gaaggtatgg accggcggct ggtcgagata gtagtagccg gcaaaggatt tatcggcttt
     3301 gtacatgaca gccagggccg tcgatggagc acagttggaa gccagcgtcg ggaaagcgaa
     3361 caaggccttg ccagcccggt cagcgacgac tttacaggta tcgatggcac ggccgccgcc
     3421 gacggcgaag accaggtcag catcctggac ggcctgttca cggagcaggc ggtcgacgtg
     3481 ttcatacgtc gcatcgtcgc cgtaccagag tacgccgagg acttccaggt ccgtcttggc
     3541 gatggcatcg agcaagggct gttttgcctt ggacagggcc gtcttgccgc cgatgatgac
     3601 gacttttttg ccgaaatgac gggtaatctt gccgatagca tcataagcat ggacgccaat
     3661 ggtaaaagcc ggcagaacca aagtatagtc agtttgagac atgataaaaa cctcctgtaa
     3721 taatgaaaaa agataagttc aaaagctata gccattatag cattgtccac tggccgtggc
     3781 tagggtaaaa ggtttaactt ttctattcgc aatacctatg aaaaatcgct atgttgacat
     3841 ttatccatat gactttcagt aattatatgg cttatctagt atgtaaatta aacgttcatt
     3901 gctatatata gtacgaaaac gtttactata acaaattgta atttgataat gtattttgag
     3961 ttataatcag ttagcgtact atgctgagtg tacatcttgc aattcgggaa aactgctcta
     4021 taataaggga gcgtaatgta gtgtacccca tttccaagcg ttgtacgttg gtatcatttt
     4081 gtttcatacg gaaagggttg cacgcatact attttatcaa aggaggttct ttttgtatga
     4141 acggtttgta tctcgttatt atttcagcat tagtgttcgt actggcttac cgtttttacg
     4201 gcgctttcat cgccgcaaaa gtcttgacgc tgaaccagta caaagtcaca ccggcattcc
     4261 gctttgaaga cggtcatgac tacgtaccga ccaacaagta cgtcgtcttc ggccatcact
     4321 tcgcagccat cgctggtgcg ggcccgcttg tcggcccggt catcgccgcc cagttcggtt
     4381 atctgcccgg cgctttatgg attctcatcg gtggggttct cgccggtgct gtccatgaca
     4441 tggtcatcct cttctgctcc atgcgttacg atggcaagtc catcgccgaa atcgcaaaag
     4501 cacagattgg ccagaagaca ggcgtagcta cttctttcgc cgtcctcttc ctgaatatcc
     4561 tctgcatggc tggcatggcc gtcgttatcg ccaatgccct ggcaaacagc ccgtggggta
     4621 ctttcaccat cggcatgacc attcctatcg ctatcttcat cggcatctac atgcagttcc
     4681 tgcgtcctgg caagatcaaa gaaggcacca tcatcggcgt catcctcacc ttgctggctg
     4741 ttgtcgctgg tccggcagtc caggcttcgc cggtcttagg gcccatgttc accatcagcc
     4801 ataacggcat ctgcatcgcc ctgatcatct acggcttcat cgccgcagcc cttccggtat
     4861 ggctccttct ggcaccgcgt gattacctct ctacgtacat gaaaatcggt actatcggtg
     4921 ccctggctct cggcatcctc atcgtaggcc cgcagattca gatgccggca acgacgcagt
     4981 tctgcgctgg cggcggcccg gtcattaccg gtgcagtcct tccgtacatc ttcgttacca
     5041 tcgcctgcgg tgctgtttct ggtttccatt ccatgatttc caccggcacg acgccgaaga
     5101 tgctcatgaa cgaacgctcc atcctgcctg tcggctatgg tgctatggtt actgaatcct
     5161 tcgtcgctat gatggctctg attgcagcct gcgccctcgt accgaacgat tacttcgcta
     5221 tcaactcttc ggctgcacac ttccaggctc tgggcatcca ggtcgtcgac ctgccgcacc
     5281 tgaacgaaat ggtcggtgaa aacgtcgccc atcgtccggg cggtgccgtt tccctggctg
     5341 tcg
//
LOCUS       DAWCYS010000095         5179 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_911_length_5179_cov_7.151639, whole genome shotgun sequence.
ACCESSION   DAWCYS010000095 DAWCYS010000000
VERSION     DAWCYS010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_5179_cov_7.151639"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1131..1140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1148 a         1450 c         1407 g         1164 t
ORIGIN      
        1 ctacgtcgtc tgcggcctca tggagctccg ccaggaggtg cgccgtcagt tcctcgccat
       61 acgggacgcg gctggccagg caggcagcgc tctgcttatt ccaggtctgg aggccgaatt
      121 ccttcgacaa agcccggata tccgccttgg ccaggcctgc ttcgatcatc gggctgcgga
      181 cggcgtcgga taattcctgc agggctttca tacccggccg gtaatcaccg aggtcatcga
      241 cattcccccc gtcgacgacc cactgatagc cttcggcctt ggccagggcc acgagcgacg
      301 taaagcgtat tttcttgcag atatagcagc gatctggcgg attctggacg aagtccgggt
      361 cctcgaattc cgtcgaatgg gccaggcggt gttcgacgcc gataaccttg gccatggcct
      421 tggcatcagc cgcttcactg cccggcaggg tcgccgaaac ggccgtcacg gccaggaccc
      481 ggtcccccag agcctggtga gccgcatagg tcaggaaggt gctgtcgacg ccgccggaaa
      541 agccgacgac gacactgccc atgtccttta agatctgctg gagtttttca tatttcttgc
      601 ttgtctctgt tgaaatcaaa agagttcccc ctcgaaccaa tatagttttc atagaactgt
      661 cgaatgacac ccttcatttc ttcgtcaccg acatatggca gggcctgctg ccacaaggca
      721 tcgggcacgc cataataggc ttcggcaatg ctgccggcaa tggctgcctg cgtatccgta
      781 tcgccgttga gccaaatcgc cttgcggatc gtatcttcat agtccgtccc ttccaggaaa
      841 cattcgatgg cttctggcac actatcgtcc gtcgtgcagg taaagccata agacggccgt
      901 atctgggcca gtgtccggga cagatcatag aaatgcgacc gcagatatgc ggcaatcgcc
      961 tctttctcct tcccctgccg ggccaggaag atagccgacg ccacggcaat ggcacccttg
     1021 atggcttggg gatggtcatg gctgacgacg gctgtcagct atgccaccgc ctgggcttcc
     1081 acctgagtat cggaaatgca ggcagcccgg cggcgccaca aggcggccag nnnnnnnnnn
     1141 cgccccattg ccatagctgc cgtaagccgg tgcctcgtcg gccaggaacc agcggcggaa
     1201 caggccgcca aaaccggcgt cgggatattt ccggcaccag tagcggagct ggcgccgcaa
     1261 gggttcgata tagccgcagc aggatgcctt cccctccatc agggctgccg ctacggctac
     1321 agtcgtcacc gtatcatcgg taaaacggct gtttttaacg aataaaggaa aatccaggga
     1381 ttccgcggtt tcatcgaatt catagatgga gccggcaata tcgccgatga tagctccctt
     1441 catggaatca tctccctaaa agaaatgagc cgattcttct ttcaagactt tatcgcgcaa
     1501 agccgacagc ttgctgctgc gctggaccca catttcttcc gggttgacca ggcgcaggta
     1561 ttcgggccac aaagtctgga cctgctggtc tgcataggcc atgatggttt tgaggtccgg
     1621 tttctgatag accagttcgc ctttctggaa aatagggaag agcatcttct ggacacggta
     1681 agtcccgcca ggaatcgtcc gcgagcgcca cggtgcctta tcgctctgga ggagcaggtc
     1741 tttcgattcg tcaatgcttt caccttcgag gatgatgatg tcagctttca ttttccccgt
     1801 atctttatcg taaatgcgca ggacgttctt caggcccgga ttggaaatct tttcggcatt
     1861 gtcgctcatc ttgatcttcg gcacgaattt gccatcgtca tactggccga cgagtttgaa
     1921 gacgccgccc agggacggac tgtccttggc cgtaatcaag ttcgtgccga caccccagga
     1981 gttgatggcg cagccctggg ctttcaattc ggaaatgagg ttttcatcga ggtcattcga
     2041 ggccgcaatg atggcatccg ggaagcctgc atcggcaaac atcttatggg cttctttcga
     2101 taaataagcc aggtcgccgc tatccaggcg gataccataa gccttgggca gggtgccggc
     2161 agccttcatt tccttgaaga tcgtaatggc atcggggacg ccctgtttca gcgtattata
     2221 tgtatcggcc aggaggatga ggttgttcgg gtactggcgg gcataggcgc ggaaagcttc
     2281 cagttccgac gagaagctca tgacccagct gtgagccatg gtccctaaga cgggcatgcc
     2341 gaatttggcg ccagccagga cgttgctggt cccgttaaag ccgccgatca tggccgaacg
     2401 ggcgccgtaa acaccggccg atttcccctg ggcccggcgc aggccgaatt ccatgaggtt
     2461 gtccttacca gccaccgtcc ggacgcggcg agccttggtg gcaatgaggc tttcatggtt
     2521 catgatcatg gacatgcacg tttctaacag taaggcttcg tccttcttgg tctggacgcg
     2581 caggagcact tcctgaggaa aggcgacggt cccttcgggc atggcataga tgtcgcccgt
     2641 aaagcgcagg tctttcaggt aatcgaggaa tttgtcctgg aaaaaaccgg tgctcttcag
     2701 ataggccagg tcgtcgtcac tgaaatgtat attgtgtaca taatcgatga gatgttccag
     2761 cccggccacg accgtatagc ccccctggaa gggattggtc cggtaatagc ggtcaaacac
     2821 gcattgctgt tcgtgcttgc cttcaaaaaa taagccctgc atcatggtca actggtacag
     2881 gtcggtcaat aacgctttcg tatacaagat aactcgcctc caaaaagtag ttattcctat
     2941 tatacatcag ttaacgataa aggaacaacg aattaccgat tttgcataca tcgcaaaatt
     3001 ctatacggac ttagcatgac aaaataggct gtcgcacgtg cgacagccta tttcatcgta
     3061 ggggccgtcc caaagggcgg ccctttctgg tccattgtta gcggatggta ttaaatttaa
     3121 cctctccggc ctttcaggcc acctcccctc tcaaggggag gctgcgagtc acgaaccacg
     3181 agccacttta cttcctcttc aacacgttat ggacggcctg gatgacgtcg ctggccgaga
     3241 gattataggc tttcaggagg tcatccggtt tccccgattc gccgaagcgg tcctggatgc
     3301 cgacgagttc tactgggaca cagcagtact gggccagggc ttcgcagatg gcccctccca
     3361 gaccgcccat gatttgatgt tcttcacaga cgacgacggc ccccgtttca gccgcacaag
     3421 tacagaccgt atccaggtcc agcggtttga ccgtgtggat gttgacgacg cgggcagaaa
     3481 taccttcccg ggccagttca tccgccgccg tcagggcttc atggaccatg aggccgttgg
     3541 cgaagagggt cacatcactc ccatcccgga agatggccgc cttatagagg tcaaaaggcg
     3601 tatccttatc ggtgacgacg ggcgccggat tgcggccaaa gcggacatat gacggccctt
     3661 tgatatcata gacggccatc gtcgccttat acgtttccca ccagtcacaa ggcgtaatga
     3721 ccatcatatg gggaatgacc cgcatgaggg cgatgtcctc cagagcctga tgggtagcgc
     3781 cgtcagggcc gacggaaatg ccggcatggg cgccgccgag cttgacgttg agattattat
     3841 aacagaccgt attgcgtatc tgtccccacg cccggcctgt caaaaagacg ccgtatgtcg
     3901 aaatgaaggg gacgaagccc gtcagcgaca ggccggctgc cgtaccgacc aggtcttgtt
     3961 ccgatatgcc gacattgata tatttttcag gatattcctg gcggatccat tcggtccgcg
     4021 tcgatttggc gacgtccgca tcgaggacga ggacatcgtc gtgaaggcgg cacagttcca
     4081 gcagggcctt cccatagccg tcgcgcatcg gtactcgttc cattacggca cctccgttgt
     4141 cagttcatcc atggccaggc ggtactgggc ttcatccggc gccgcaccgt gccatttcgc
     4201 ttgattttcc atatatgata cacccttccc cttgaccgta tgggccagga tgaccgtcgg
     4261 cttgccgcga taggccttgg cagcgccata agcgtcgtac agggcatctg tatcgtggcc
     4321 atccacgcgg aggacatgcc agccgaaact ttcccatttc gcatccatcg gttccatatt
     4381 cttgatcgac accaaggaat cgacgagctg caggccattg caatcgacaa tcagacaaac
     4441 attgtccaaa tggtgctgtg aggcaaaagc ggcagcttcc caaatctggc cttcctgctg
     4501 ttccccatcg ccgatgagac agaagacttc cggtgtttca tggcgcagtt tcaaggccag
     4561 ggcaatgccg ttggcaatgg acaggccttg gcctaaagag ccgacagcgc agtcgagaca
     4621 gggcagtttt tccaaattgg gatggccctg gagggcactg ccaaccgtcc gcagcgtatc
     4681 gagcaaggcc agggggaaat agcccttatg ggccaaaatg ctgtaataag ccggggccgc
     4741 atggcccttg gataagacaa aacggtcccg ttccggcgca tggggccggt caggaaaaca
     4801 ggtaaagaca tctgtaaaat aaagtgtggc catgatttcg acagccgata gagagctgcc
     4861 aggatgaccg gaaccggcac ggtaaatgga ccgcaccgta tcccgccgta tctgcataca
     4921 cacgtcatgt agatcatgct tcatgaagca tagcctccta tgtcaggtcg tcaagaaaaa
     4981 aacgccgcat acccccgttt atgtctggcg gcgatacgcg tgcgttcgga catacgacct
     5041 cttttgttcg tttctatttc ttgagtttat tgtacaatga ttatatacac aagtcaaaca
     5101 gaatatacca tctggatatt cttcatgaat gtatagaaag acgtgattac ataatataaa
     5161 taaatgttat aaaaatgga
//
LOCUS       DAWCYS010000096         5161 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_917_length_5161_cov_5.187818, whole genome shotgun sequence.
ACCESSION   DAWCYS010000096 DAWCYS010000000
VERSION     DAWCYS010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_5161_cov_5.187818"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1237 a         1574 c         1338 g         1012 t
ORIGIN      
        1 cgtcatcccg gcgctgctgc ccgaccaggt cacgatgtat gcctagccct ttgaaggcta
       61 ctggaaggat ctcggcacca tcgacagcgt gtggcaggcc aacatggacc ttatcgccga
      121 cgaaccgcct atcgacctgc gcgacagcaa gtggcgcatc tattcgggca accagagttt
      181 accgccgcac ctcatcgggc ctaacggctc ggctaagagt tccctcaacg ctgaaggcac
      241 ggaaatcttc gggactgtca gcaattcagt catcttctac ggtgtcacca tcaaagaagg
      301 ggcgaaagtc accaattccg tcatcatgcc cggctctgtc gtcgaagaaa atgccgtcgt
      361 cgacaaggcc atcctgggcg aacgctgcca catctatccc ggcgccgtcc gccacaatga
      421 agacgggtct gtcgccgtac tgggacgcaa aggggaactg aaaccaaaac aagttacagc
      481 gaaaggggaa cactagttat gaatagtaat atgatcggcc tcatcaatat gcgcgaagac
      541 catcccttgc aggaaatcaa cgacacgcgc ccgctgtcga cgctgcccat cggcggcaaa
      601 taccgcctca tcgacttcac gctttccaat atggacaacg ccggcatcga aaacgtcggc
      661 ctcctgctct ccagtcagtc ccggtcggtc ctggaccaca tccggtccgg caaggaatgg
      721 ggtctggccc acaagggcga cggcttgttc tatctgccgg aagaacgggc ggacatcgaa
      781 catcccgtag aaggggacat cagtgcttat tataagaacc tcatcttcgt ccagcgggcc
      841 aacaagcgct acgccctgct gtcgggctgc gacatggtcc agaacatcga ctatgacaag
      901 gtcctccact tccatcgtca tcacaacgcc gacgtcaccc tcatctatca gcagcagaaa
      961 tacgacttcg accgcgaagg ctatgtcctg accatcgacg atgtctgccc ggaccgggtc
     1021 acggccatcg aagccaagcc ggaagtcaaa gctggcgata atttatacca gcggggaatc
     1081 ctcatcgact gcgatgtatt ccagcattgc atacgtcggg cctatgcccg gggatatacc
     1141 cattttatta ccgatgtgct ccagcgcaat gtagaccgcc tgcggatttt cggctataat
     1201 tatcagggat atgcaaaacg gatcgattcc gtcaattcct acttccaggt caacatggac
     1261 ctgcgcgaca gccgcatctg gcatgaactg ctcctcaagg atatggatca ccgcatctat
     1321 accaaaatca aggacgaagc gccggccaaa tacatggaag aatcgcatgt ctccaattct
     1381 ctcgtagcta atggctgcat catcgaaggc cgcgtagaga attcgattct tttccgccgc
     1441 gtcaaggtcg gaaaaaatgc tgtcattcgc aacagcatca tcatgcagca ctcggtcgtc
     1501 ggcgatgacg ctcagctcga ttacgtcgta tgcgacaaga atacggtcat ccagccggaa
     1561 gctgtgttga gcggcagcca cgacaacccg ttgtgcattg gtaaacgttc agtccgctaa
     1621 acgcaactat gaaggggtta gagacaatat gaaaaaatgg caggataagg aagaattcaa
     1681 aaagttattt accgagaaag ctcacatctt gtgggaaaaa gatctgcggg acatgacctt
     1741 gaatgaagtc tatcagacca tcgcctatat gatccgcgat ctggtaagtg aagattggat
     1801 ccgcacgaat gaaatctatg ccgaacagaa ggttaagcaa gtctattatt tctccatcga
     1861 attcctcatc ggccgcctcc tggaatcgaa tctcctgggc atcgatatgg acgacatctg
     1921 tcgccagggc ctggaagaca tgggctggga cctgaataag gtcatcccgg ccgaacgcga
     1981 tccgggcctg gggaatgggg gactgggccg gctggctgcc tgcttcatcg attccctggc
     2041 agcgctgcag ctgccgggcc atggcaacag catccgctat cagtacggct tgttcaacca
     2101 gcgcattgtc gacaaccagc aggtcgaact gcccgataac tggctggtca acggctatcc
     2161 ctgggaagtc aagaaagtcg acaaagccgt ctatgtccgc tttggcggca acgcctacat
     2221 gcgtcctgac gaagacggca acctggaatg tgtccacgaa aagtattcgt ctgtccgcgc
     2281 cgtaccgtac gatgtgccta tcatcggcta ccacaacaat acggtcaaca cgctgcgcct
     2341 gtggcgggcg gaatattcgc gggaacagct ctaccaggaa ttgtccatcg gcgaccggcg
     2401 ccgggccttc cgctacaagg acagtgtcca gcagatttcc cgtttcctct atcctgacga
     2461 cagcaatgaa gacggccgcc gcctgcgcct gatgcaggaa tacttcttcg tatcggccgg
     2521 cctccagagc atcgtccgcc attataagaa gaaatatcac gaaagcatct ataaattcga
     2581 ccgctatgtg gccatccaca tcaacgacac ccatccggcc ctggtcatcc cggaactgat
     2641 gcgcatcctc atggatgaag aaggcatatc gtgggatgcg gcctggaaca tcaccgtccg
     2701 caccgtggcc tatacgaacc acaccatcct gccggaagcc atggaaaaat ggtctatccc
     2761 catgttcaag gaactgctgc cgcgcattta cctcatcgtc gaagaaatca acaaccgctg
     2821 gctcaaagaa gtccgcagcc tctatccggg cgatgaatcg agagcccgcg acgtcgccgt
     2881 cctctgggat ggccaggtcc acatggctca tctggccgtc ctcggcagcc acagcgtcaa
     2941 cggcgtcgcc gaaatccatt cgcagatcct caaggattcg acgctgcacc agttctacac
     3001 gtgcttcccg caccgcttct cgaacaagac caacggcatt tcccatcgcc gctggctcat
     3061 cgaagccaat ccgcagctgg cgtcgctcat cgacgatacc atcggtgaca gctggcgccg
     3121 gacgccgtca aagctccagg acctcatgcc ctttgccgac gacccggcct tccaggaaca
     3181 gctgacgcgg gtcaaaaagg accgcaagga aatcctggcc cgctacatcc gcgaccgcta
     3241 cgatacggac gtgcggacgg actcgatctt cgacatccag atcaagcgca tccacctgta
     3301 caagcgccag accttgaaca tcctgcacat cctgcacttg tactacctct tgaaggacaa
     3361 cccgaaactc aagatcacgc cgcggacctt cctctttggc ggcaaggccg ctgccggcta
     3421 cggcgaagcc aaggaaacga tccgcctcat caacgtcgtc gcccatatgg tcaattccga
     3481 ccgtaaggtc aacaagcaga tgaaggtcct gttcctggaa aactacaatg tttccttagg
     3541 ccagctcctc ttcccggctg ctgacgtgag cgaacagatt tcgacagccg gcaaggaagc
     3601 gtcgggcacg ggcaacatga agttcatgat gaacggtgcc atcaccctgg ggaccatgga
     3661 cggggccaac gtcgaaatcc accgcaaggt cggcgacgaa aactgcgtca tcttcgggct
     3721 gcgggctgac gaagtcatga actactacat ccacggcggc tactcgtcgt ggcagatgta
     3781 cagcgacgac gccgccatcc gccgcctcat ggatgccctc gtcgacggct ctatcgacgg
     3841 ctctatcggc ggggaccact tcggtatgct ctactcggcc ttcctcgacc ggaacgacga
     3901 atacttcgtc ctcaaggact tccagcccta ctgccaggcc cagcaggaaa tcgccaaacg
     3961 ctacggcgac catacgaaat ggtgtcattc gagcgtcgtc aacatcgccc agtccggcta
     4021 cttctccagc gaccggacga ttcagcagta cgccgatgac atttggcgca tcaagccggt
     4081 caaacactaa ctaagaatca ggaggataag atatgaaact cagtgcaatc gacgagtaca
     4141 gtaccttctt gtatcatcag ggtacgaact acgccagtta tcgtctgttc ggggcccatt
     4201 tcacggtcct gcgccgcaag ccctgtgtgc gctttgccgt ctgggcgcct cacgcccagc
     4261 aggtcagcgt cgtcggtgat ttcaatcact gggacgccga tgccaatccc atggagcgga
     4321 gcagctgcgg caacggcatt tgggttatgc atattgccgg cctccaggaa ggggacatct
     4381 acaagtacgc catcacgaag gcgtcaggcg aacgcatctt aaagagcgat ccctatgcct
     4441 tctggtcgga agtacgtccc aatacggcat cgaaactgac caagctccac tacacgtggc
     4501 gcgacaagaa atggatggac agccgcagtg cttacgattc gtaccgtaag cccatgctga
     4561 cctacgaagt ccatctcggc tcgtggcgcc gcggcgaaga cggcgaaatg ttgtcgtacc
     4621 gcgatatcgc gccccagctg gtcgattatg tcaaacagat gaattatacc cacatcgagc
     4681 tcatgccgct gtgcgaatat cccttcgatg gttcctgggg ctaccagggg acggggtatt
     4741 tctcggcgac cagccgctat ggcagcccgg aagacttcat gtacctcgtc gacctctgcc
     4801 accgcaacgg catcggcgtc atcctcgact gggtaccggg gcatttttgc cgcgacgccc
     4861 acggcctgcg ccatttcgat ggcgaaccgt gctatgaatc gggcaacccc atgctggcag
     4921 aaaacaagga atgggacacg atgaatttcg actacggccg gacggaagtc cagtccttcc
     4981 tcatttccag tgccctgttc tggatcagcc agttccacat cgacggcctg cgcatcgacg
     5041 ccgtcgccaa catgctctat ctcgattacg gccattccgc cggcgccagg caggccaata
     5101 agtacggcgt ccgggaaaac ctggaagaca tcgagtgact gctccagctc cagccggacg
     5161 t
//
LOCUS       DAWCYS010000097         5047 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_939_length_5047_cov_6.901042, whole genome shotgun sequence.
ACCESSION   DAWCYS010000097 DAWCYS010000000
VERSION     DAWCYS010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_5047_cov_6.901042"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1296 a         1352 c         1239 g         1160 t
ORIGIN      
        1 tatgttccat acgttctaca accactgccg catcatccag gaagacaaga atctggaaag
       61 agcccgtctg gccctggtca cggcagtccg catcactatc gccaacagtt tggctatttt
      121 aggggtttct gctcctgaaa aaatgtaata taataaaata aaagttattg attgattttt
      181 gattcgatga gcagaagctc aacagggagg aaatatggct aaatacatct ttgtcacagg
      241 tggcgtcgtt tcttcgttag ggaaaggcat tacagcagca tcgctgggcc gtttgctgaa
      301 gaaccgcggc tacaaggtca cgatccagaa gtttgacccc tatatcaata tcgacccggg
      361 cacgatgagc ccgtaccagc acggcgaagt cttcgtcacc gacgatggca cggaaacgga
      421 cctcgacctc ggtcactatg aacgcttcat cgacattaac ctcggcaaga actccaacgt
      481 cacgaccggc aagatctact ggtcggtcct gcagaaagaa cggcgcggtg actatttggg
      541 ccatactgtc caggtcatcc cgcacatcac caatgaaatc aagaaccgcg tctaccgcgt
      601 cggcgacgat gacaatgcgg acatcgtcat caccgaaatc ggcggtactg tcggcgacat
      661 cgaaagcctg ccgttcctgg aagctatccg ccagatcaag aaggaagtcg gccgcaatga
      721 tgtcttgtac atccacgtca cactcgtacc gtatatcggc gctgctggcg aactgaagac
      781 caaaccgacc cagcacagcg tcaaggaact gcgcagcatc ggcatccagc cggacatcat
      841 cgtctgccgg acggaacgcc ccttgtcgga tgaaatgaaa gaaaagctgg ccctgttctg
      901 cgacatcgac aaagatgccg tcatcgaaaa caagaccttg aacagcatct atgaagtacc
      961 cctcctcttg tcggaagaag ggatggaccg catcgtcctg gaaaaactcg acctgcccaa
     1021 tcgtccctgc gatatggaag actggaagaa aatggtccat gccatttaca atacggaaaa
     1081 gaccctggaa atcgccttgg tcggtaaata cgtcgccctc cacgacgcct acctcagcgt
     1141 cgctgaagct ctgaaccatg ctggcatcgc ctataagacg aaagtcaata tccactggat
     1201 cgattcggaa accctggaag acccgaacct ctcgatggaa gaagtcttca aaggcatcga
     1261 cggcattgtc gttcccggcg gcttcggcaa ccgcggcgtc gaaggcaaga tccgtgccgt
     1321 ccagtatgcc cgtgaaaaca acgtgcctta cctcggcctc tgcctgggta tgcagtgcgc
     1381 tgtcatggaa ttcgcccgcc acgtcgtcgg cctcgaaggc gctacgtcgt cggaattcca
     1441 tgaagatgcc aaattcccgg tcatcgacct catgcccgac caggtcgaca tctccgataa
     1501 aggcggcacc atgcgcctcg gtctctatcc ctgcaagctg accgaaggta cgaaggccca
     1561 tgaagtctat ggcaccaacc tcatttacga acgccatcgc catcgctggg aattcaacaa
     1621 taaataccgc acggaaatgg tcaacgccgg cctcgtcctc tcgggcacat cgccggacga
     1681 ccgcctggta gaaatcgtcg aactcaagga tcacccctgg tttgtaggct gccagttcca
     1741 tccggaattc aaatcccgcc cgaccaaagc ccatccgtta ttcaaaggcc tgatcaaaac
     1801 ggctttagaa ttaaaagggg aataattagt gggggccgcc gcatgaaggg ttctgccatc
     1861 atgccaggcc ccttttaatt tatagtttgt agtacggagt ttgtagaaga tggctttaaa
     1921 tttaaaaaac ttcactaaaa actacaaact gcaaacttaa tactcccaaa ggcgctgtca
     1981 taagacagcg cctttttcgc gtctatgcgg cagacgcata taggatgcat atatatgtga
     2041 tttattgaac gtatggcaag ttgttctaga gccgtcaaaa tacgataaaa gttagttacg
     2101 gcgacatgaa aaataaacta ttttttgtta ttatgcatcc ttataaaata gggtaagaac
     2161 ggcctttata ctatacaaaa caggtgacgt atacggaaat gtaaactacg ttatctcttt
     2221 gtatccggac aatttttgac ttctttttcc tgcgcccggc aggtcataaa aataagttaa
     2281 atgtgtaact ttgctctgat tttcacattg aataatatgc tacataatga tataatgtaa
     2341 gcgattgaaa gcatgattca tgtttttatt atttttcatt atataataat gaggtgattt
     2401 ttgatgttga atacctttaa aagaggtggg gtccatcctg acgatggcaa gatctatgca
     2461 aaagacaaag ccatagaaac cccggcagta ccggctcaag tcgtcattcc catgagccag
     2521 cactttggtg cgccttgtac gccgaccgtc aaagtcggtg acctggtcaa gaaaggccag
     2581 cttatcggga cgagtgacgc attcttgcat gccgacatcc atgcatctac gtccggcaaa
     2641 gtcatcaaag ttgcgcctat gccgcacaac atgatggtca aatgcatggc tgtcgtcatc
     2701 gaagcagatg gcaaagatga atgggcagaa gggattcccc aggctcacga ctggaaacag
     2761 ctcgacagca aggaaatcct ggaactcatc aagaaagccg gcgtcgtcgg ctgcggcggt
     2821 gctactttcc cggctcatgt caaattggct ccgaacaaac cggtcgatac gttcatcgtc
     2881 aacggtgccg aatgtgaacc gtatctgact tgtgactatc gtgccatggt cgaaaaggaa
     2941 acgacggaca aactcgtcac gggcgtccag atctgcatga aagtcctcgg cgtcaagaag
     3001 gctttcatcg gcattgaaga caacaaaccg gaagccatcc agaacctgac cgaagctttc
     3061 aaagatattc ctgaagttac ggttctcgct tgtaagacga agtaccccca gggtgctgaa
     3121 aagatgatga tcgaagcctg cacaggtcgt caggtcgaac cgggcggact tcctatgaac
     3181 gtcggctgcg tcgtcagcaa cgtcggtacg gtcattgcca tcaccgatgc tgtctgccag
     3241 ggcatcccct tcatcgaacg ccttacgacc gttaccggtg actgcatcaa agaaccgaag
     3301 aacctccgtc tccgtatcgg tacgactttc caggaagcca tcgactactg cggcggcttc
     3361 tccaaacagc cggatcgcct gatcgccggc ggcccgatga tgggtctggc ccagtatcag
     3421 ctcgatgtgc cgatcaccaa aggggcttcc ggtattctgg ctctctctcc ggaaaaatgc
     3481 caggtcggcg aagaatctcc gtgcatccgc tgcggccgtt gcgtagaagc atgtcccatg
     3541 ggcctcattc cgagccagct cagcatcttc tcgtcctgcc aggcatggga taaatgcatg
     3601 gaatacggcg tcatgaactg tgttgaatgt ggcagctgtg tctatacctg cccggcaaaa
     3661 cgtaacattg tccagtatat ccgcaatgcg aaaggccagt gcaaagctat ccagcgcgca
     3721 gcgcaggcta aagctgaagc acagaaagcc aagaactaat tggaagaagg gatatcatga
     3781 gtccagaagc taaaggacaa acaaatggaa aggatgctaa agacatgcag gaatttaaac
     3841 ttaccgtagc atcttcaccg catgttcgct gtaacgaaac cattcctaag atcatgtgga
     3901 atgtcgtcgc cgcccttgta ccggctgctg cgttcggcgt ctactatttc ggtgtcaatg
     3961 ctctcgtcaa tatcatcgtc gcaattgtct cggcagttgt atttgaattc tgatgggaaa
     4021 aactgatgca caaacgcgtc gccctcaaag atggttctgc cgtcatcacg ggccttctcc
     4081 tggctatgtg ctgcccgccg agccttccct ggtggatgag catcatcggt tccttcctgg
     4141 caatcgtcgt ttgtaaacag gccatgggcg gccttggcta caacatcttc aacccggctc
     4201 acgtcggccg tgctggtttg atggtttcct ggccgatcgc catgacgacc tggacccagc
     4261 tcaatgggac cgtcatcgac ggtgtcgctg gggctacacc gctgaacgtc ttcaaacacg
     4321 gcggcgtaga tgctctcttc aatctcttcg gtaccaacga ttggggcagc atttatcaga
     4381 gcttattctt cgggttccgc aacggttccc tcggtgaaac gtcgacagtc ctcctcatcc
     4441 tcggtggtct gtggctgatc tacaaaggct acatcaactg gcaggttccg gtcatcatga
     4501 tcgctaccgt cggcgtcctc atgggtgctt tctatggcgt caacgttgca atctttgaaa
     4561 tgatggctgg cggcctcatc atcggcgcct tcttcatggc aaccgatatg gtcacggctc
     4621 cgattaccct gaaaggccag atgatcttcg cattgggctg cggcctgatt acggtcctca
     4681 tccgtactct cggcggttat ccggaagggg tttgctactc catcttgctc atgaatacct
     4741 tgactccgct catcgaccgt ctggtcaaac cgaccgtctt tggcgcagta aaaaaggggg
     4801 ctaaataaaa tggcagacaa aaacgaaagc ggaatcttca aggttgcgat gaaccttatc
     4861 ctgacctgcc tggtttccgg ctgtatcatc ggcgtcgtct atgctgctac cagcaagatt
     4921 gcctatgaaa aagctgaaca gatgaaacag gattccatga actccctcgt tcatcaggct
     4981 gaaacattcg atcccggccc tgccgatata gatacctgcg tcgctgacga gggcagccag
     5041 aacgtgg
//
LOCUS       DAWCYS010000098         5046 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_941_length_5046_cov_5.257263, whole genome shotgun sequence.
ACCESSION   DAWCYS010000098 DAWCYS010000000
VERSION     DAWCYS010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_941_length_5046_cov_5.257263"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1005 a         1445 c         1429 g         1167 t
ORIGIN      
        1 acgtcttcga tttcgccgac cgcctggcca aggaaatcat ggtgccccgc caggtcagcg
       61 gctgcctcta tacggaagat actatgaacc agcatttgca gaccatccgc cagtcgcggc
      121 atacgcgcta tcccctctgt gaagacgata aggaccacgt cctgggcctg gtccacatca
      181 aggacatcat ggacctctat atccacaagc gcagcaactt gaacctcatc aagcggccga
      241 tcctcatggt cccggaaatc atgccggcat cgaagctcct ccagctcatg cgcatgcgcc
      301 ggacctacct ggccatggtc gttgatgaat acggcagcac cgtcggcctc atcggcctgg
      361 aagacatcct ggaagaactg gtcggcacga tccagaacga acacacccag gaaaaagaag
      421 aaatccagcc cctgcctgac ggcgcctatg aatttgccgg gacagtcctt ctcgacgacg
      481 tcgaagagct gctgcacatc cccgtcgaag acgatgtcga taccgatacc atcggcggct
      541 acgtcttcaa tgccctgggc catacgccga aagtccacga cgaagtcact atcggcgcct
      601 actgctttac agtcctggaa atgcagcgct tccgcatcgt ccgcctgaaa gccgtacccc
      661 tgccgggagc ggctgcccct ggggaagaca gcggggatga agaagataag actcatgaat
      721 cgtagcggct actatggccg caaaccgtcg cggtcctatg aaggcatcat cctcaccgtc
      781 cgcaagacca gtgccaaaca gctcgtcgtc acggtcctga cccggcaggc cggcatcctc
      841 cacgtcctgg cccgccgcag cactcagggc aagatgggct acggcgcctt gacgcctctg
      901 gccgagatga cctttgacgt ctttgaaaaa gacggcgtct acacgctgac ggaatacgac
      961 tgccgcagca accagcgcct ccgcgagctg acttgggacg gctatctctc ttcgaagatc
     1021 ttcgtcgaaa tggtcctctt cctggccggc ggcggagccg atgaagtcct gtacgatctg
     1081 gtccgcctct atagccgggc catcgaactc aaggacatcc gcatcgtcac catcatcgcc
     1141 ggctggcagc tcatggccct gacaggcttc ctgccggaca tcgacacggc ccgcgtcttc
     1201 tgtcagggcc agggctactg ccggcagccc gtctattaca tcgacgatga aaggccggaa
     1261 aacatgaaag aactgcctct gacgccgtct gtacgccaga cctggaagac cctggtccac
     1321 tatccctggg gaacccaaga aaccgtgaac ctgcgcatgg ccgacctgaa attcctggaa
     1381 gccctgctct acaactacgt caaccagtgc agcgaacgcc agatgaagac accggaatta
     1441 ttagtggctc gtggctagtg gttcgtggat cgcggatcgc ggatcgtagg ggcggcccac
     1501 ctggctctct tgataggaga gctgtcagca aagctgactg agaggttgct cttttaaata
     1561 gttcgtgttt cgtaggggtc gccacgtggg cgacccgtta gggatttgtg tgattcttct
     1621 agcagactgg tcgggaaatc gtacatattt cagagcgggc gccccacgtg gacgccccta
     1681 caaaaaagag gctgtcgcgc atgcggcagc ctctttttgc cccctcaccg cttatgcagc
     1741 gggtcgtatt tccaatgatg ggcgtattct tttagacttt cggcgacggc gtattgggcc
     1801 gtatcggtat agaccatttg cgtatctacg gactcttccc agatggggac aggccagtcg
     1861 cggggcggtt cgatgtaata gagcaggaat tccctggtct tttcgatgaa ccattccggc
     1921 aggcgggggt ggaatttctt gtagaagatg aacaagtgga tgtgattggg catgaccgtg
     1981 aactctttga tgatggcaat ctggttgttc cgctgcatgg ccttgagggc ccgcgtcgcc
     2041 gccatgcccc aggcggtcgg ccgccagatg tgctgtccct gatagcgcag gactttcccc
     2101 agtgtttctt ccttttgata cgtacaaaag gtgatgagct ggcaccgggg aacggtagag
     2161 aagggttccc gccatgcagg aagggagaaa aacggtttgt gatacatggg caacacctcc
     2221 gaaaaaaaac aaaacgttta aaacaagatg cgtgttacgc ctattgtacc ctggaaatat
     2281 gaaagggaca ttaaaaaatc aggggccttg attaaaaaat gattaaaact tgtttttgtg
     2341 ccctatcatg ctataataaa actatttcac tccttgttat tagagaaagg acgattccat
     2401 ggttcgcagt atgacttcgg gcaatcccat ccgcctcatc ctggctttta cggtgcccct
     2461 tttaatcggc aatgttttcc agcaatttta taatatcgcc gacatcatca tcgtcggccg
     2521 cactatcggc gtcaatgccc tggccgctgt cggcgccgtg gcacccgtct tcatggccgt
     2581 cttcggcctg acgatcggtt tgtcttccgg tttttccgtc atcacgggtc agcgcttcgg
     2641 tgccgaagac atggacggcg tgcgccggtc tgtggcgacg tctgccatgc tggccctggt
     2701 catcaccctg atgctgacgg cctgcgtgag cctggccatg ccgctcatca tgcggctcat
     2761 gaacatttca gatgtcctct acgacgacgc ctatcattac atgctcatcg tcgtcctcgg
     2821 cctggtagcc atgatgagct ataatctcct gtcctgcatc tgccgggccc tcggcgacag
     2881 ccggacgcca ctttatttcc tcatcgtgtc gtctctcttg aatatcgccc tggccctgtt
     2941 gttcatcgtc gcctttggct ggggcgtccc cggctcggcc atcgccctgg tcatcgccca
     3001 ggccgtatcg gccgtgctct gcctgtgttt catgcgccgc cgctttccca tgctgcgcct
     3061 gacccgagcc gactggaact ttgactggcc ctttgcctgg cagcatttgc gcctgggcct
     3121 gcccatggcc gtccagttcc ttatcatttc catgggcatc ctggtcctgc agtcggtctg
     3181 caataccttt ggccctgaaa cgattgccgg cttcgtatcg gcgacgaaaa tcgaacagct
     3241 ggccttgcag cccatgattt ccttcggcat cgccatggct gtctattcgg cacagaacta
     3301 cggcgccagc cagtatggcc gcatccgcca gggcgtccgt cagtgctccc tcgtgtccct
     3361 ggtattctgc ctggccgcag ccgtggccat gtacagctat ggccgggaac tcgtcagcgt
     3421 cttcacgacg gaccacgacg aagtcctcat ggaacaggct ttcctctatc tgaagatgtc
     3481 cgtcccgttc tatctcttcc tgggccagat cttcgtctac cgcaacgcat tgcagggcat
     3541 gggcatttcc agcgtgcccc tcatcagcag catcctggaa ctgggcggcc gcagcgtatc
     3601 ggccctggtt ctggccgcca tgtggggcta cttcggcatc tgctgtgcca gccctatttg
     3661 ctgggtcctg gcctgcctat ttacaggtgg ttcctatttc tgggtcatgc acaccatgaa
     3721 ggccaacggc tgttaaaaag ggaaggggac agtctatgaa tacagagaaa gaaaacaata
     3781 tgacttcgtt aggagtatga agcatttttt tagcaagctg attgggaaaa cgtacatatt
     3841 acaaagcggg cgctccgcgt ggcgcctcta cagaaaaagc gccgtccgcg aggacagcgc
     3901 tttttttcta ttcaaactga ttgactaatt ctatgagtct ttctttatgc tggggatacc
     3961 attcgttgta ggactggttg gcatcgaaaa gggtctggtt gagggccagg aagaagtcgg
     4021 gcaggtgctc gatggtgatg accccgattt gttcgcccat gacgccttta ccgatggcgt
     4081 agttgcctcc ggcgtagatg ttgaaggccg gttccatttt gtcgccgatt tttttgctgg
     4141 cgccgcgcag gcccagagga gccagctgct ggaccgtgca gttcgacgga cagccagaga
     4201 agtggcattt cggcaggaag tggctgtcga tgccgcggtc tttgaagagc tgggccagtt
     4261 tgacgaaggt gccgtgagaa tcgtgcaggc cctgctggca gaccgtagcg ccgatgcagc
     4321 tgacggaatg ttcaaaggcc gtcgtcgccg tgtcgtcctc cgtgagggca gcgactttac
     4381 gtgcgtcgtc tgccgtgagt ttgatgatgt aagccgtttc gtcggcattg aggcggattt
     4441 ctgcgccttc catggtctgg agggccttga gcaggtcgtg gaagacggag agtttcggcg
     4501 tgccgcctaa aggcttgtat tcgacgtaat agaggccggc ctgtttctgt ttgccaatgc
     4561 ggccgtcttc gaggacgctg tcatcggcgc cgctcttagc taacggcgtt tcttcttcga
     4621 tggtcaggcg gtaatcgcct tcggccttgg ctttggcgac ggcatcgcgg acggcctggc
     4681 ggaaggcatc gtcgccgagc ttggtgagga tgaaacgcga acggttctgg ccgcggtgtt
     4741 catagtcgcc gtatttcatg tagacgtccc ggccatagcc caggatgtaa taggaaatat
     4801 cctgcggatc gacgccttcg tcgatgagga tgccgaagcg cgagccgttg agtccgaagc
     4861 cgccgccggc atagacgtcg aatttgtggt ccggccgggc cacgaagccc aggtccttgc
     4921 aggtcgcatg gccttcgttg tccaggccgc gggagaagct gattttatat tttcgcggca
     4981 agttgaattt gggaatgcac ttgatgacgt agttggctgc tgccttgatg tacggcggca
     5041 gcggca
//
LOCUS       DAWCYS010000099         5025 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_949_length_5025_cov_6.864386, whole genome shotgun sequence.
ACCESSION   DAWCYS010000099 DAWCYS010000000
VERSION     DAWCYS010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_5025_cov_6.864386"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1100 a         1375 c         1350 g         1200 t
ORIGIN      
        1 cccatgggcg aagacgatag gaatgctact atcggagcga gcagctgcgc cgtccggcgg
       61 gcccgttcga cacagctgac agcaatagcg gccagctgct gcggcgtcgg ctcgatgacg
      121 acactgcagt cgccgacgac gaagataccg gcatcgccga actggggctt atcggtaatc
      181 atgatgaaag aactgctgac cgtcgacagg ccgggatgag gaccgatgac ctgcagagcc
      241 gcccggatga cttccgacga cgtggccacc gcaccggcga ccatgccgtc ggcaatatcg
      301 aaggcaacca gacaggctgc aaagaagatg taattttctg acaggacttt ccgggcttct
      361 tccaggctca tgcccttctt agcccgccgt ttggcataat attggcagaa cttatccatc
      421 atggggtacg tttcagggtc gatgatttca atcccgtcca tatcgatctg gtattccgaa
      481 gcgacttcga cgatactccg gggacggccg atgaggatgg gccgggcaaa gccttcggcc
      541 tggacccgtt cggcagcctg caggacgcgg cggctttccg attccggcag gacgattttc
      601 ttctgttctt tagcaacgcg gcgtttcagg ttatttataa atcgtgacat aagacaacac
      661 ctcacatcta tctacaatca tcactgtcat ttcgttaagt atagtatacg gcaaaacgaa
      721 gacggccgcc tcttttccat gacgaaagga gatattattc tgccgaacgg agaataaatg
      781 ggacaaaaaa gaggctgtcg ctgggacagc ctctttttta gttcgaagtt tgtagttttt
      841 agtttgtagt gaatgtttaa aaatttaaaa ccgtcgtcta caaactgcaa acgctgaact
      901 acaaactatc tcttattcta cggtaacgct cttagccagg ttacgcggtt tatctacgtc
      961 gttgccgcgg ctgacagcga tgtaataggc caggagctgc aaggggatga tggccaggat
     1021 aggaacgacg aaatcgtccg atgccggtac tttgatcaaa tcatcgacgg tcttgccgac
     1081 ttccgtatcg ccatcctgga cgacgccgac gacataggca tcgcgggctt tgacttcctt
     1141 gatgttgctg atcatctttt cttcgacttc cagctgggta gccagggcga tgacggggac
     1201 accggcttcg atgagggaca acgtgccgtg tttcagttcg ccgccggcat aggattcagc
     1261 atgaatatag gagatttcct tcaatttcag ggcaccttcc atagccaggc cgtagtccat
     1321 ggaacggccg atgaaaaaga cgtcttcttt atctttgaaa gtactgcaga gacctttgat
     1381 gaaatcgacc tggtcgaaga tggcctggca ctgatccggc agtttggtca aatcagccag
     1441 gattttttct tcccggtcta cgggcaggtt cccgttcaag cggcccatgt acagggacag
     1501 gagcaggagc gtgcccagct gcgtcgtata ggccttggtc gaagcgacgg cgatttccgg
     1561 gccagcccag gtataagcga cctggtcggc ttcacgggaa atagacgaac cgacgacgtt
     1621 ggtgacggcc agggaacgag cgccacggcg tttggcttct ttcagggctg ccagggtgtc
     1681 tatggtttcc ccggactgac tgatgacaat gcacagggtc ttgctgtccg tcaagggatc
     1741 gcggtaacgg tattccgaag cgatgtcgac ttcgacgggg atgcgggcca gtttttcaat
     1801 gaaatacttg gccaaaaggc cggcatggta ggccgtaccg caggcagtaa tcaaaatctt
     1861 gccgattgat ttgacgtctt ccggcgtcca gttcaattcc tcaaaatgga cgtgcttgcc
     1921 atctttggtg atgcggcagc tcatggtatc tttgatagcc ttcggctgtt cgtagatttc
     1981 cttgagcatg aagtgttcaa agccgccctt ttcagctgct tcagcatccc agttgacttc
     2041 aaagaccttc ttggaaatgg gcttgccttc gaggtccgtg acccagacat tatccttcat
     2101 gacgatggcg atttccccat cgttcatgat gtacgtcttg cgggtgtagc tgaggatggc
     2161 cggaatatcc gaagcgatgt aattttcccc ctggccgagg ccgacgatga ggggattgtc
     2221 tttcttgctg caaatgatct tatccggttc aaacttgcac atgaagacca acgaatagga
     2281 accgcgcaga cgggccagga ccttgcggac ggtgctggtg aaatctccgt cgtacatgtc
     2341 ggcgcagagg tgcgcgacga cttccgtatc cgtttccgac ttgaagtgat accctttttt
     2401 gaggagttct tccttcaatt ccatatagtt ttcgatgata ccgttgtgga cgatggcgaa
     2461 gttccccttt tcatccgtat ggggatgggc attgacgtcc gacgggccgc cgtgtgtcgc
     2521 ccagcgggta tgaccgatgc ccagcgtccc tacgacgggg tgagccttga cttcttcagc
     2581 cagattgacc agacggcctg cttttttgcg gacagcgatg gcgccgtcgt tgtagacggc
     2641 gataccggcc gagtcataac cacgatattc cagcttggcc agtccatcca tcaaaaaatc
     2701 tgctgcttct ctgtctccga tgtaacctac aataccacac atagttatat cctcctgata
     2761 tgaaaatgtt ccttcttatg acgaacttgt cgacgacgtt accatcgtgt ttttccgcca
     2821 tataacggct gaagctgccg agagacatcc gctgactatt tcgatacttc tctacctcgt
     2881 ctgcctgaat ccatcaggca ctagcgctat ccattatatc gtcaaggatt tccagacgcc
     2941 tgcataaaaa agtgcaggaa agagttccag gaactccatt cccacacttt aatatatttg
     3001 caaggttttg tcaataccta cttagacggg cccatttcgt gttcgacgac gacggcgatg
     3061 tcatcgacga tgcgttcgag ctgttccagg tccggtcctt ctgccatgac acggatcaag
     3121 ggttctgtgc ccgacgggcg gacgaggatg cggccttcat cgcccaactc ttcttcgcca
     3181 gccgagatgg cggccatgat gaggttattg tcttcccatc ccgatttcgt ctgaacgcga
     3241 acatttttca aaatctgcgg atagcgcgtc atcaatccgg ccagttccga caagggcttc
     3301 tgctgctgtt tcatgatgct cagcagctgg accgccgtga gcaggccgtc accagtcgta
     3361 ttgtagtcga ggaagatgat atggcccgac tgttcgccgc cgagggaata gccgtctttg
     3421 cgcatctttt ccaggacata gcggtcgccg acagccgtaa tttccgtctg gcagcccatt
     3481 tccttcaggg ctttgtggaa accgatattg ctcatgaccg taccgacgac ggtattctgt
     3541 ttgagccggc cttcggcctt caatttcatg gcgcagagga gcatgatctg gtcgccatcc
     3601 atttcctggc ccttttcatc gacagccagg cagcggtcgg catcgccgtc gttggcaatc
     3661 cctacgtcgg ccccatactt gagaaccgtt tcctttaagc tttccaaatg ggtagagccg
     3721 cagttgtcat tgatgttgat gccgttcggt tcatgatgga tggcaatgac ttcggcaccc
     3781 aggcgtttga ggatttccgg ccccaagacc gaagcggaac cattggcccc gtcatggacg
     3841 actttcaggc ccgacaaatc gacatccgtc gatttgcaga tgaaatcggc atattcctgc
     3901 tgtaaatcgg tccacgtcga aatcgtgccg atagcggccc cggtcggacg gctctgaagg
     3961 gcagcccgtt catctttgag catacattct tcgattttgt cttccgtttc gtcaggcagc
     4021 ttatagccaa agccgtcgaa gaacttgata ccgttgtatt caaagggatt atgcgacgcc
     4081 gaaatcatga cgccggcatc catatgatga gtccgcgtca agtaggcaat ggccggagtc
     4141 ggaatgacgc ctaccatgta gcagtcacca ccagccgaag cgataccggc ggccaggata
     4201 tctgccagca tagtgccgga acggcgggta tcccggccga tgagaaaggt cgggtgttct
     4261 ttgttctgcc caaaaatata ggcagctgct ctgcccaaat gatataccaa ttccggtgtc
     4321 agcgaatcat tggcaatacc tcgtactcca tcagttccaa acaatctagc catttacttt
     4381 acctccaaag gttgatattt acggatgatt tcaatcgccg tttccagtcc atccaaaaaa
     4441 gcgctcataa tgccgccggc atagccagcc ccttccccga tgggataaag gcctgccgcc
     4501 cccagggcca tgcggtcttc gccgcggaca atgcgtaccg gggcgctggt ccgggtttcg
     4561 acgccggtca tgacgacatc gtcatcgtca aaaccgtgaa tccgccgccc aaaataaggc
     4621 agggcctgtt ccagtgtcgt cgtaacgaaa cctggcagga cctcatgcag gtccgtccag
     4681 gtcacgccgg gacgatagga atggatgggc ccttcctgcg gtgctccagg acggctgagg
     4741 aaagccccga cggtctgagc cggcgcgtga taattgctac cgccggcctg ataggccagg
     4801 gcttcatagc ggcgctggaa ttcgatcccc gccagggggc tgtcgccgcc catgtcgtcg
     4861 gctgtgacgt tgacgacgac agcactgttg gcgatgccgc tgtcacgtcg gtacaggctc
     4921 atgccattgg tgacgacccg cccttcttcc gaagccgcgc cgacgacgac accgccggga
     4981 cacatgcaga aagaatagac tccgcggccg ccggattgcg ccaca
//
LOCUS       DAWCYS010000100         5019 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_951_length_5019_cov_5.865028, whole genome shotgun sequence.
ACCESSION   DAWCYS010000100 DAWCYS010000000
VERSION     DAWCYS010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_951_length_5019_cov_5.865028"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1059 a         1350 c         1392 g         1218 t
ORIGIN      
        1 ctacttcttc ggcgacgtcc ggcatttccg tgcagtcgcc gcgatagctg ggaaggacgg
       61 ccgtcaaggg cacgcagtct gtttcattgc cgccctgcag ggtttccagg agattgacca
      121 tgtcggcttc cgggatttcc gtgcccagga atttgttgac ctgagccgcc gtaaaggcga
      181 tgcgcgtcgt agcctgcggt ttcggataga catcgacgac gccttgggct acgtcgcagg
      241 cgcccatttc ctggaggagc tgggcacagc ggtcgatgga tttgatgcag ctgtccgaat
      301 cgagtcctcg ttcaaaacgg ccggaagctt cggaacgcag gccgagcata cggcccgtat
      361 gacggatgtt ggagcctttg aaggaagcac attcgaggat gaccgacgtc gtctggtccg
      421 tgacttccgt atccatgccg cccatgatgc cggcgacgca gcaggctttt tcttcatcgg
      481 cgatgaccag catgttgtcg gtcaatttac ggtctacatc atccagagtc ttcaaggctt
      541 catcggcttt ggcccggcgg gcgatgaggt gatggccggc gactttatcg tagtcatagg
      601 catggagtgg gatgcccagt tcgatcatgg tgtagttggt agcgtcgacg acgttgttga
      661 tgggacggac gccggacttg cgcaggcgct gctgcatcca cataggcgac ttgccgatct
      721 tgacgttcag gagcaggcgg gcggcatagc ggctgcacag ttcttcgtcg tcaatggata
      781 ctttgacctt gccgtcgatg ggcgtgtcac attctttgac ggaaatatcc gggtaacggg
      841 cttctttgcc gctgaggacg gcgaattcac gggacaggcc gaccatggag aagcagtcgg
      901 cgcggttggc cgtcagttca tattcataga cgacgtcgcg gatctggaga tagtcggccg
      961 catcgacgcc gaggggcgta tcgggcggca ggatccaaat gccttcccgt tcttccggca
     1021 agagcaggct gtcgtcgaag ccgaattcgt gggccgagca gaccctgccg ttggacaaga
     1081 cgccgcgcag cttcgagcgc gtgatctcag tgccgcccgg cagatgagcg ccgtggacgg
     1141 cgacggggac gatctggccg acgcggacgt tggtcgcagc cgtgacgatt tggagctgtt
     1201 catcccccat gtctacctgg cagacgacga gtttatccgc atcggggtgc ttgtcgatct
     1261 gaaggatctt cccggtcttt actttcttga cttctgtccc ccagtattcc acctgttcga
     1321 cagggatacc ggcaatggtc aatttatcgg caaatgcctg catgtcctgg ttcatgtcga
     1381 cgtcgacata atcctgcatc caagttaaag aacttttcat tattcatacc ctcctagaac
     1441 tggttcaaga accgcatatc gttttcatag aacaagcgca aatcatcgat gccgtatttc
     1501 aacatggcaa tgcgttcgac gcccatgccg aaggcaaaac ccgtgacgac gtccgggtca
     1561 tagccgttca ggcgcaggac gctgggattg accatgccgc agccgaggat ttccagccag
     1621 cccgtgtggg agcagacacg gcagcccttg ccgccgcaca tgacgcagga aatatcgact
     1681 tcggcgctcg gttccgtgaa ggggaagtag ctggcgcgga agcggacttt cgtctgcggt
     1741 ccgaagatgt cgcgcaggaa gtcttcgagc atgcccttga ggtcggagaa gcggatgtcc
     1801 ttgtcgatga tcatgccttc catctggtgg aagaccggcg aatgggtcgc atcgtagtcg
     1861 cagcggtata ccttgcccgg tgcgatgatc ttgaagggtt cgttaggctt gtgggcctgc
     1921 aaggtccgtg cctgcatgcc cgatgtatgg gtccgcagga ggatgttatc cgtaatgtac
     1981 atggagtcct gcatgtcccg ggccggatgg tcttccggca gattcaggca ttggaagtta
     2041 tagtaatccg attcgatatc cgtcccttcg acgatagaga agcccatgcg caggaacgag
     2101 tcttccatca ggcgcagagt ctgatgaagg ggatgttcgt ggcccatgac cggtttgcgc
     2161 cccggcaggg taatgtcgat tttttccgat tcgatcttcg cagccaattc cttggccttg
     2221 acttcttcca tcttggcctt gagctgttct tcgatggcat tgcgggccgt gttgacgagc
     2281 tggccgactt taggccgttc ttctttggaa aaattcttca tttccttcat gattgccgtc
     2341 agttcccctt ttttacccag gaacttgacg cggatatcct gtacgtcctg aagatgttcg
     2401 ctcttggcga gctgttcatt gacggccgct ttgatggcct cgatcttatc actgatcata
     2461 ctttcttcct cctactggca agtacaaaaa aaatagactc ccgcccccta aggggcgaaa
     2521 gtctcattcg cggtcccacc ctacttgatt ctcgattgtc ctgtaacggc aacccccgtc
     2581 ctgcctcctc cggtcagcag gaggctctaa agtgaacgca tggaacatcc ggcacgaggc
     2641 tctcaatctg cggcctcgat tccctgtagg cggagtcagt tccatcccct ctttatcatt
     2701 gccataaatc cctctatggg tttgttatat attgtatcag taaagtgtct tatttgcaat
     2761 aaccaaccgc tgaatttggt tggtgccttc gtaaatctgc atgattttgg cgtcacgcat
     2821 gtatttttcg acagggtatt ccttgatata gccatagccg cccatgacct ggactgcatc
     2881 ggtcgtgacc tgcatggcca cgtcggacgc gttgcacttg gcaatggccg cttcacggct
     2941 gaagtccata ccctggtctt tcatccagca ggcgcggtgg accaggaggc gggctccttc
     3001 gactttcatg gccatatcgg caatcatggc ctggaccatt tcaaaggaag cgatgggttt
     3061 gccgaactgt acgcggacct tggcatattc gcagcaggca cggaaagcgg cttcggcaat
     3121 gccgacggaa acggcaccga cgaagggacg ggctgcatcc aaagtatcca tggcgatgcg
     3181 gaagccatgg ccttcgcggc cgagacggtc ttcgacaggg acgaagcagt cctggaagat
     3241 gagctccgtc gtattggaag cgcggatgcc cattttattt tcagccttgc cgactgtgaa
     3301 gcccggcgtc cccttgcgga cgatgaaggc cgtcaagccg cggatgccgc tgctcttgcg
     3361 ggtgttggcg aagacgacga agacttcggc cagggcgccg ttggtaatga agcatttcgt
     3421 accattgagg atatagcctt tgccgtcttc ggtcttcatg gcccgcgtcg aaacggaacc
     3481 ggcatcggaa ccggtgcccg gttcagtcag ggcaaaagcg gccaggttgt cttcattgat
     3541 gatatcgaaa tagcgtttct tctggtcgtc gttaccggca atgatgacgg gatacgaggc
     3601 cagggcattg gcagcgacgg tcgtagcgac gccggcacag cctttgccta attcttcata
     3661 aatagcggcg atagtgatgg cgtcgaggcc ggcaccgccg aattcttccg gaatggcgac
     3721 gcccagcagg ccctgggctg ccaatttttc caggagccct gcatggagtt ttcccgtttc
     3781 atccatttcc gaagcgtagg ggacgatttc tttttccacc aggtcgcgga caaccttgat
     3841 gatttctttc tgttcgtcgt tataagcaaa atccatcttt gtactaacca accttcccta
     3901 taattacatg attatcctat caatcgagtt ttttcaaaat ataaaaagac gaccgccctt
     3961 ttacgataat attaccgctg gcatcataac attcacattc gacgacaatg gtcttccggc
     4021 cgtggtgaat gacctggccc acgccggtaa tgtgctcgcc gcctttgaca ctgcggatat
     4081 agttcatgtt gatttccagc gtcgtcacct ggaagcccag ggtccggctg gccatgccca
     4141 tgagggtatc gcagaaagcg gccaggacgc cgcccgaggc aatgccccgg taattgcagg
     4201 aatgacggct gatatcgaag ccgatgcgga cgcggccgac gtcgagcttt tctaccgtac
     4261 agggcatgct ctggaaataa ggtgtatgcg aataaatact gtccagcata cgatgcagcc
     4321 gttctgccac ttccgggctc actgcttctt ctggttcatt catccgtatg ccccctttct
     4381 ccctcaaaga cgtaggcccc tgtcacaaag aaggtgccct tgccatgagc taaatagcgc
     4441 tggtcattat cgtaaaaatc acagagggcg accatcgtcg tacggccgtt atggatgatc
     4501 ctggcttcgg cccggatgac cgtattggca aaagccggac gcaggtaatt catacccagg
     4561 tccagtgtga ggacggcttt gccacagaca gcacaagcag cctccatcgc cgaatcggcc
     4621 atggaataaa ggacaccgcc gtacatggta ccattgggat tatcccattt cgagccttta
     4681 ttctcaaaag tgaggatcgt actctctggt tcagcctttt cgacgtggaa atccataaag
     4741 gtatcgaagg gatttttgtg aatttctcgt tccaaccgct tagcatccat tatgactcca
     4801 ctccttaggc taataacata aataccctta cattttacta tacgaacaat catccgtcaa
     4861 acgaaaatac aaaaaagagg ctgtcacatc aagacagcct cttttaaggg gctaggggct
     4921 agtggctagg ggctagtggt tagcggatgg tttgaaattt atggccatct tctacaaact
     4981 ataaagaaaa agggactgcg catgatggca gaatccttc
//
LOCUS       DAWCYS010000101         5018 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_952_length_5018_cov_9.243200, whole genome shotgun sequence.
ACCESSION   DAWCYS010000101 DAWCYS010000000
VERSION     DAWCYS010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_5018_cov_9.243200"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1104 a         1504 c         1427 g          983 t
ORIGIN      
        1 atttctatga aaggtaattt attcatcatc gaaggcggtg acggcagcgg caaagcgacg
       61 cagacccgcc tgctgacgga acacttgcag cgcgacggct atgacgtgaa gtccgtcagc
      121 taccccgact atgacagcga ctccagcgcc ctggtcaaga tgtacctgcg cggcgacttc
      181 ggcagcgatg ctgatgccgt caatccctat gcggcgtcgg ccttcttcgc cgtcgaccgc
      241 ttcgcgtcgt atcagatgaa gtggaaagcc ttctacgaac agggcggcat cgtcattgcc
      301 gaccgctata cgacgagcaa catgctctat cagatgatca agatcgacga cccggcccag
      361 cgccgggctt atctggcctg gctcgaggac tttgaattcc gcaagatggg cctgccggca
      421 ccggacaagg tcttcctcct cgacgtcccc ctggctgtca cggagcggct catggctgac
      481 cggacgggca agaccggcgg ccagaccggc gatatccacg aaaagaatga aggcttcctg
      541 gccaaatgcc acgccgctta tgatgagctg gcccaacgct atcactggca gcgcattgcc
      601 tgcacggaag gcggggccat gcgcacgcct gaagccatcc atcaagactt atatggctgc
      661 ctatttactt tgtaggggat tttttctata attaattgtt aaggtatttt attggcgtaa
      721 gggggcgaca tgatggacag gacactgttt gatccgatta tcggccacag ccgcctgaaa
      781 gaacagttcg cccgcctcat cgacgaagac cgcatccctc atgccatgat ttttgccggc
      841 ccggctggta tcggcaagac cctgatggcc atcgccgtcg cctcggccct ggtcggacgc
      901 cgtgtctttc ccgacctggc cagccgcgaa ggcgaagccg tagccggcga taaagacgat
      961 gccttctatc tggcgccgat gggggccatg ctgaaagtgg accagttccg ccagctccag
     1021 ggcgaactgg ccctgcaggg cgaaaccggc cgccgccggg tctgtatcat cgaccatgtg
     1081 gagacgatga atacagaatt tgccaaccgc atgctgaaga ttttagaaga accgccgtcg
     1141 ggcgtctgct tcatcttgat tacggaccag cccgatttgc tgctgccgac catcatttcc
     1201 cgctgtgccc gctttacctt cgaccccgtc ggcgacgacg aaatgcgcca gggactgcgg
     1261 cgcctgcggg gcgacggcgg gaattgggac gaagccatcc tctggggtgg cggcatcgtc
     1321 cggactgtat tgtcctatct cgacggccag gggacggata aggcccattt cgccctggat
     1381 tttttgacga ctacggcgaa acatgcctgt ccctatgcca aatggctggc cctgtccacg
     1441 gcgctgacgg atagggaaac gacggagata ctcggctgga tcagcctttt tctccgcgac
     1501 atggccgtcc tgcgcagcgg tgccggccgg gactatatac gtttaaaagg atatatccat
     1561 gaaatgacag aattactgcc gtattggacg gatgacgccg tctttggcct ttcccgggtg
     1621 ctcgatgaag ggctggaagc cgtctcgcgt cacgtcaacg tccgcctggt ctgggactat
     1681 gtatgcctcc aggccatacg gcttagagga ggaattcaat gatcgttgta ggtgtacgct
     1741 ttaaacctgc cggaaagata tattatttcg atcccgccac tatcgaactc aagctggatg
     1801 acggcgtcat cgtcgaaacg gcgcggggca tggaattcgg ccatgtcgtc attgcgccgc
     1861 gcagtgccga ttcgtcggaa gtcgtccagc cgctgaagcc gatcattcgc aaggcgacgg
     1921 ccaaggacat tcgccaggtg gaaaagaaca aggaacggga aaagaaagct ttcggcatct
     1981 gcctggcgaa gatcgccaag cacaaactgc ccatgaaact catcgacgtc gactatacct
     2041 tcgacatggg caagattatc ttcttcttta cggctgacgg ccgcatcgac ttccgcgaac
     2101 tcgtccgcga cctggcgtcg gtcttccgca cgcgcatcga actgcgccag atcggcctgc
     2161 gcgacgaagc caagatgatc ggcggcatcg gctgctgcgg ccggcccctg tgctgctcta
     2221 cgttcctggg cgatttcaag cccgtttcca tccgcatggc caagggccag ggcatgtccc
     2281 tcaatccgac caagatttcc gggatctgcg gccgcctcat gtgctgcctg cgctatgaaa
     2341 accagctcta tacgtcgggc gagctgaagt gctgcaactg ccagcagaag agctgtgaac
     2401 agcagaagcc gccggctgtg ggcaaacggg tcatgaccga cgaaggcgtc ggcacggtcc
     2461 tgcgcgtcca catgaaggac catacggtca aagtccagct cgaatcggga cacaacatca
     2521 acgtgccctg gtcggacata ggggaaacgg aacatgaaca ttgagttagg cccgcacgaa
     2581 cggctggacg acctggtcct ggacggcatg aaagtcattc agcgcgatga ccagttctgc
     2641 ttttctctgg atacggtcct tctggcccac ttcggtgccg tgcctcacgg accggctttg
     2701 gacctgggga cggggacggc ggctatccct ctcatcctga cggcccgcgg tgccaaggcg
     2761 ctgacggcag tggaattcaa tcccgtcatg gccgacattg cccgccgtaa cgtcgccctc
     2821 aatggccggg acgaccgcat taccatcgtc gaaggcgact accgccgcat ccgggagtgg
     2881 gcggcgagcg gcgcttttgc cgcagtctac gccaacccgc cgtaccggga aaagagccgc
     2941 ggtgcctata gcgacgtcga cggcatccgc cgggcctgcc atgaagagac ggcgaccctt
     3001 ggcgacgtcc tcgatgctgt aaaatacgcc ttgaaatatc acggccgctt ccgcatggtt
     3061 catattacgg aacgactggc cgatatttta gaggccatgc gcgcccgaga tatcgaaccg
     3121 aaagtattgc agatggtcca cggccggcct gacaagaagg ccaagctctt cctcgtcgag
     3181 ggcatccgcg gcggcagcaa gggcatggac gtcatgccgc cgctcatcgt ccacaacccc
     3241 gacggcagct acagcgatgc cgtcctgcga ttatacggga aacgataact agggaggaat
     3301 atgatgccgg aaatctttca gaaagggacg ctgtatcttt gcccgacgcc tatcggcaat
     3361 ctcgaagaca ttacgtaccg cacggtccgc tgtctccgcg aagccgacct catcgcggca
     3421 gaagatacgc gtcatacgaa acagctgctc ctggcgtatg atatcgatac gccgttgacc
     3481 agctatcatg aacacaataa agctgaaaaa gggccgcagc tcattgaaaa attgaaagac
     3541 ggcctgatga tcgccctggt cagcgatgcc ggcatgccgg ccatctgcga cccgggcagc
     3601 gatatggtcc gcctggccct ggaagccgat atccccatcg tacccctgcc cggcgccaat
     3661 gccggcctga ccgggctcat tgcctccggt atggatacga cgcgctttac ctttgtcggc
     3721 ttcctgccga agacccagaa acaccgcctg cccgtcctgg aatcggtgaa agcctatgaa
     3781 gggacgctca tcttttacga agcgcctcac cgcatccagc aggtcctggg cgaaatgctc
     3841 gaagtcctgg gcgaccgccg ggccgtcctc tgccgggaac tgaccaagcg gtatgaacag
     3901 tacctgcgcg gcagtctcag ccagctgcgg gccgacctgg ctgagcaggg gacgcgcggt
     3961 gaattcgtca tcctcgtcga aggggcggcg cctgctgccg aatccgctgc ctcaggcgac
     4021 gtcgattacg cgtccctggt ccgggaactg atggaacagg gcgtcgatag gaaagaggcc
     4081 atccggaccg tcgccagacg gtgcggcgtg cctaaacgta gtgtatacca ggctgcgctt
     4141 agtttataac atccggctca tttgccggta taggaggaaa atcccatgtc tgaaaaaaga
     4201 aagtattaca ttacaacacc tatttactat ccaagtgcca aacttcacat cgggcacacg
     4261 tactgcacga ctattgccga ctcgctggca cggttccacc gtctgcgcgg cgacgacgtc
     4321 tttttcctga ccggctcgga tgaacacggc cagaaaatcc agcgtgcagc cgaagcaaaa
     4381 ggcgtcaaac cgattgaata cgtcgacgaa atcgtcgccc tcttccagca gctctggaaa
     4441 aaactcaaca tttccaatga tgacttcatc cgcacgacag aaaaacgcca tgaaaaagtc
     4501 gtccaggctt tgatgcagaa gtcctacgac aacggcgata tctataaagg tgaatacaaa
     4561 ggctggtact gtacgccgtg cgaaacgttc tggccggaaa acaagctgcc cgaaggcaaa
     4621 cacgtctgcc cggactgcgg ccgtccgctg gaactcgttt cggaagaagc gtacttcctg
     4681 aagatgaaca aatacgccga ccgctggctg aaattcatcg acgaaaaccc ggacttcatc
     4741 cagccggaat cgcggcgcaa cgaaatgatt tccttcgtca agagcggcct ggaagacctc
     4801 tgcgtttccc gtacgtcctt cgactggggc atcaaggtcc cctgggaccc gaaacacgtc
     4861 gtctacgtct ggttcgacgc cctggtcaac tacgtcacgg ctgccggcta tctcgatgac
     4921 ccggaaaaat tcaagaaatt ctggcccgct gacctgcatc tggtcggcaa ggtactcgtc
     4981 cggttcgacg gcttcttctg gccgatgagg gtgctgag
//
LOCUS       DAWCYS010000102         5015 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_953_length_5015_cov_4.773185, whole genome shotgun sequence.
ACCESSION   DAWCYS010000102 DAWCYS010000000
VERSION     DAWCYS010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_953_length_5015_cov_4.773185"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1228 a         1429 c         1403 g          955 t
ORIGIN      
        1 cacctggaga ctccctacgg ccccgaccct cgcccagagc agaagtactc tgtcacaatc
       61 aggctgccta agtcggacgt ggaaggcaag cagaaagtcg acgctgctat cgcggctgcc
      121 acgcgcaacg gcatcgccaa caaatggaac ggcgcagccc cggccaaagt accgacaccg
      181 gtatgggacg gcgatggcct gacccagaac gggaacaaat ttggcccgga atgtgctggc
      241 cattgggtat tcgccgcatc gacgccggcg gataagcctg tcgacgtcgt agacggccgt
      301 atgaaccgca tcatcgacgc tacgcaggta tacagcggca tctatgccaa tgtgtgcgtt
      361 aatttctttg cttacaacca tcagggcaag aagggcatcg gctgcggcct cggcccggtc
      421 cagaaggtcc gtgacggtga acccctcggc gggtccgcgc cgacagcgaa atccgtattc
      481 cacgtcattc aggaaccagc ggcaccgtct gcgcctgcta tcaatcctct gaccggacag
      541 ccgatgtaat gcaatcccag gcgcgtggac ttccgcgcgc ctatttttct ctataggagt
      601 gaatccggat gaaacacgaa catctatcca tcgacatcga aacattttca gatgtcgata
      661 tcgggaaatg cgggcttttt aaatacgtcg atacagatgc ctttgaaatc ctgctgtttg
      721 cgtactcgta tgactttggc ctggtccacg tcgtcgacat gacgaagggc gaaatcatcc
      781 ctgcggacgt cttgcaggac cttgcagatc ccggcgtcat caagcacgca tataatgcgg
      841 cttttgaaat cacctgcctg aaccgtgccg gctatcggac gccccctgat cagtggcggt
      901 gcacgatgct ccatggtctg taccttggct accctgccgg gctggctaat ttgggaaaag
      961 ccttaggctt gccggaagat aagcgcaaaa tggctgccgg caaggcgctg atccgctact
     1021 tctgcgtacc ctgtaaagca acaaagaaaa acggcggacg gcatcgcaac cggcccgtgc
     1081 acgacccaga caaatggaaa acctttaaag cctacaacgc tcaggacgtc gtcacggaaa
     1141 tggccgacta tcagcggctc atggcctatc ccgtgccggg ttgggtccag gaagattggg
     1201 taattgacta tgagctcaac cgccggggca tccaactcga catggacctt gtcaaagggg
     1261 ccatcgccat cgacggggac taccgcagcg cgctcatcga aaaagccata cggattacgg
     1321 gcctgcgaaa tcccaacagc cgaaatccgc tgctggcctg gctcaacgaa aacacggact
     1381 tagagcttga aaaactgacc aaggaaactg tcgcggaatc cttacaagtg gccagcgggg
     1441 atgcggaaga ggtcctgcgg atccgcaaga atctatccaa gtccagcgtc acgaaatacc
     1501 aggccatgga ccgcgctgtc tgctccgacg gacgtatccg cggcgtcctg caattctacg
     1561 gcgccaatcg tacgggacga tgggcgggcc gtctggtcca ggtgcaaaac ctgccgcatg
     1621 acgtcccgca agccatcgcc acggcgcggc atctggtcaa atcgaaaaac cgccgcggcc
     1681 tggagctgat gtatggcgac gtagccagca cattgtccca gctcatccgc acggcgttca
     1741 tcgcgcccga cgacgcgatg ctgtgtgtct ctgacttctc ggctatcgag gcgcgcgtat
     1801 tgtcgtggct ggccggtgaa aagtggcgta tggacgtctt tgccaacaat ggcgacatct
     1861 actgtgcctc agcgtcgtcc atgtttggcg tccccgtcgt caagcacggc gtcaacggcc
     1921 atctccggca aaaagggaaa gtcgcagagc tggcccttgg ctatcaaggc ggcccgaatg
     1981 cgctgatttc catgggcgca ctcaagatgg gcctgatgga agacgaactg ccggatatcg
     2041 tccgccgctg gcgggcatcg aatccacgga ttcaggattt ctggtacgcc gtcgacgggg
     2101 cggccatgtc tgtcatgcaa aacgcccagc ctgtggggct gccgcacggc atcatcttta
     2161 gccgggaatg caacctgcta tacggctatg actacttgac ggttacactg ccaagcggcc
     2221 gccggctttt ctatccgcag ccgtacgtcg gggaaaacca gttcggacgg cctgcgatac
     2281 actacaaaac gcagatgggc gcgaactggg tatacacatc gacctacggt ggcaagcttg
     2341 tcgaaaacat cacgcaggca attgcccgcg actgcctggc cctggccctc aagcgactgg
     2401 tacagcacgg ctacaagctg ctcatgcaca tccatgacga ggtagtctta gaggtcccta
     2461 aggaccaatt gcacgacgac gaaatcgacc gtatcaacgc gattatgtgt gacccgattc
     2521 catgggcgcc ggggctgatc ctcaatgccg atggcttcat cagcccgtat tacaagaaag
     2581 actagaaatg atgaacaata tggctaaatt ttatacgcct gaagttgata gcgctattac
     2641 aaatgtacat attaatattg aacacgtaac atactttaca actactaagc tcgatgacgg
     2701 ctcagttgat gttgcttttt actttggcac aggccctcat cgtagcactc cgccgacaat
     2761 taaagtcaat atagatgaag acgatgtaga ctatttattc tcatgcatgc aggataagga
     2821 gatgtttgtt gatgtctaag gggaaatgca tcatctgcgg cgcggaattc gacaggcccg
     2881 cgcataacgg ccgctcggtg tactgcgacg actgtcgcaa tcgtattgtc agcgcctacg
     2941 aagcccgcat caaggcacat gagctaaagc gcatgtcgaa ctgcctcatc tgcggggcgc
     3001 cgaatcactc gaccaagatg ctgacctgct gcgaaacgca cgcgcggatg ctcaacggta
     3061 tcctgtcggc ctgccggaag acgcgtatgg ctgcatacgc cagagaacgc cggaaaaagg
     3121 cgggagtccg gaagtgcact atacacctaa gaaaaagccg ctttgtaaag atcgaccagg
     3181 acgcgcacga cgcaaaagcc gcgggcatgt cgtacggcca gtacatggcc ctgaaagcac
     3241 agaggaggga cgccacatga tgaaccgggc acgcattgcc gctaacggcg gcacgatatc
     3301 gacttccgta ctcatctaca tggccctgca cgacggcatg ggattcggga aaaaacggct
     3361 cgacgcgatt accgaaaaag taatggccta tagcgaggac atcaaccaca gcgccgaaaa
     3421 gatgacggcg taccgcaacc gcctcgatga ctgcggaatc gacttccagc taaaaatgga
     3481 tttcatccgg acgcttatca agggcctgca attcagcggc cagaaagagc ggaccggcgc
     3541 ggaagcgggc atggaagtga cctatacctt ggtcctcctg gccgtccatg acctgtacgg
     3601 cctcggccgc gtacgactgc ggcgcataca ggaaaagata aaggattatg catggtatat
     3661 ccgcgacggc gtcgtccatg tcctggaata catgaaatgc ctgacctgcg aatgcgggca
     3721 gaagtatcag gccctcatgg gctatgaaaa agagcacggc gaaataaaga tttacggata
     3781 gaaggtgata cgatgagcaa gtatattgtt tggcggttac tgctgcttta cgttactgtt
     3841 ttctggaccg catttttcgc gatggttatt tatatttgca ggtgagacta tatgaaatgg
     3901 gtgaaaccaa cagaagaaat gccggcccgg cacaaagtta tcctttttgc catcgactat
     3961 ccccggaagg accggccgcg gacgattacg acgggcattt ataccgggga ttgctttggc
     4021 tggtatgccg acgtcaacgg taaatacctc gacacggaac agataattta ctgggcgccg
     4081 attgcctcgc tgccgagaga atactaagga ggtgttaact tgcagaacga cagaaagctg
     4141 ctgatcagcg tcggcagcca tcgctactcg aaagaatgga tacaaacggc gataatgtgg
     4201 tccaagctca tcgagcgcct gcgcatccca ctgcggacgc cggaaacgta cgagaactac
     4261 atgaaactgc cgaaacgaca gaagggcgaa ctgaaagacg tcggcggctt tgtcggcggc
     4321 acgattaatg gcagccagcg caaagcgtcc gctatcacgg gccgcgacct catcacactg
     4381 gacctcgaca acatcgccgc cggggaaaca gataacacag tccggcgcgt ggacagcctg
     4441 ggcatcgcct acgccgtcta cagtacgcgg tcccatgcgc catggcggcc gcgcttacgt
     4501 gtcatcatgc cgctcgataa gactatcagc gccgacgaat acgagccgat agcccgtaag
     4561 ctggcaagcc tcatcggtat cgagctgtgc gacccgacga cgttcgacgt gtcacggctg
     4621 atgtattggc cgtcgtgcag ctcggacagc ccctacgcat tccagtacgg cgataagccg
     4681 ttcgtcagcg ccgacgggat actggcccag tacggcgact ggcgcgacgt ccgcacatgg
     4741 ccgcaggtcc caggcaagga agtcaagccg aaagagctgc tggccaagca gcatgacccg
     4801 acgacaaagg gcggcgtcgt aggggccttt tgccggacct acgacatcat gagcgctatc
     4861 gagcacttta taccgaatgc gtatgagccg acggacaagg acgaccgcct gacctacacg
     4921 ggcggctcga ccatggccgg cgccgtcatc tacgacggcg gcaaattttt atacagccat
     4981 cacgcgacgg acccatgcag cgagcagctc gtcaa
//
LOCUS       DAWCYS010000103         5008 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_956_length_5008_cov_5.795074, whole genome shotgun sequence.
ACCESSION   DAWCYS010000103 DAWCYS010000000
VERSION     DAWCYS010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_956_length_5008_cov_5.795074"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1033 a         1385 c         1467 g         1123 t
ORIGIN      
        1 ggtcaggcag cccatgacga agggcgcgtt gtgggcgacc agggtctgac tttcgatgag
       61 gtttttcagg ccggcctgcc agtattcgcc gaattcatcg gccttggaga gttcgtccca
      121 ctgatagcgg cctttgacgg cagacaagtt gttttctggc ggacggatgt agaagtaacg
      181 cgtatccttc agttcgccgt ggtcgaattc agccagggcc aggagacaga cggaataggg
      241 ctgttcgttg gccagttcca gggcgacggc cgtgaagtgc tgcgggaagc ggcgcagttt
      301 ccctttttct tcgatgggat atttgtaata gccgtcattg ggcttgatgt cgtactggtg
      361 caggttggcg actttgacgt gttccttctt ggccagttcc tgctggacct tcgccttgcg
      421 atggcgcttg cgcagggccg acgtgaggga atagaggacg gcagcgatga agacgatagt
      481 gacgatcgtc gtaatgatgt ggatcaggga atccatggaa tatcaaatcc ttttcaataa
      541 tttttgagcc ttattcttgg aagaaattca tgtcgtaaca ggcccggaaa gcgcggttgg
      601 ccgcattgcc gttggcagac aggcggtctg tcgtcttgac ttccggattg ggcagggaca
      661 aggtggacac gacgttgccc tgcaaatcgt agccgacatc gccgagagtc tgtgttttta
      721 cggtctgggc tttaaaattg tagaagaaat tgttatcata accttggacc gtgttgttgg
      781 catagtcata ggtcacgacc gtagcccgga tgatgtagta cggcggatcg tagcggacgg
      841 actggatggt gttcatgtcg atatacgatt cggtcgtgtc atcgtggccg acctggacga
      901 agcgggggtt gtcatcgaga aatgtggcgg atgccatgaa actgaagacg caggcaaatg
      961 tgactgccag agcggcaatt tttttgaaca taagacatac ttcctttccc tgtctcttcc
     1021 aatcgaggca gtttatacat atattatatc ggaatcatga aacattacaa gaacgtatat
     1081 ttttcaagta ggacttttgc cctttcgggt cgaataataa ataatataag atagaagagg
     1141 tgatcatatt ggatgtgata tatattggcc tgcctttctt cttttggcag gaagatgagt
     1201 cggagcacgg cctcgatgtc cacgtgacgg aaggcttcca gaagctgggt tcccacgtgt
     1261 accccctcaa tgccggcgac gacgcggaag aaatctgttc ggcttataat tggcatacat
     1321 cctttgtcga tgaagacgaa gacattacgc cgtcggaaga attcatttca gagcatgtct
     1381 tgtgggacga cttccgcctg ctctatattt cggcagctgc ggctacgtcg gaagacgaat
     1441 acacgcagtt cgtctgtcat acggcagagt tcgccaaaga gagcggcctc gtcgtggcag
     1501 cggaagtcgt cgacggcgac gatgaagacc cgtatccatg gcgcgataag gcgacggtcc
     1561 tctggtcccg cggcgatatc ctgccatcgg gaggtccggc ctgtgccgtg cgcttggccc
     1621 tgggcagggg catcaccatt gccagccagg gcgaagaacg gacctatgac gtccaggtcg
     1681 tcagtgaatg tttcatccct tcgtttttac aaggattgct ggaagggcgc gatccctttt
     1741 ccatcataga atcttatgta tcttaagtta ggaggtattt atcttgaaca gtaaaaaaat
     1801 ggaagaacat cctttgcggg cggccattct cactatcagc aacagccgca ctttttcaga
     1861 cgatacgaac ggcctgctca tccagtcggc cttggccaat tacggtcatc aggtcgtcga
     1921 ctatcagatc gtcaaagacg acaaggccga aatcgaacgc catctccatg aatgggtctg
     1981 cagcgtcgac ctggtcatca ccagcggcgg tacgggcatg gccaagcgcg acgtgaccat
     2041 cgaaacggta gaagccctgt tcgataagaa aatcccggct ttccagtcga tgatgacgta
     2101 tttcgcctat cgccgggcct gcggcgtcca ggccatggcc taccggacga cggccggtat
     2161 catccaccag tgccaggtct actgcctgcc gggcctgccg agcctcatca aaatcggcat
     2221 ggaaaaggtc attttgccgg aagcctttca tttgtattcg gaaatcaaga agtaaatgga
     2281 tacgtctaaa atcatacttg tcaccggcgg ggcgcggagc ggtaagagcg cctttgccga
     2341 acgctatgct gccgcccttc ccgggcggca tgcttatgtc gcgacggccc gcatcttcga
     2401 tgaagaaatg gcccagcgca tcgccatcca ccgcaagcgc cgcccgtcgt cgtggcagac
     2461 ctgggaaatc ccgcaggacc tgccagagac gatggaacgg ctgtgccagt cgtcggacgt
     2521 ggccctcgtc gactgcctga ccctctattt ttctaattat ctctttgccc acgacggcga
     2581 agacgacgaa gctatcatcg acggcgccct ggccgaactg caagcggtcc tggcggcttt
     2641 ccgccaggcc ggcgtgacgg ccgtcctggt cactaacgag ctgggctgcg gcatcgtgcc
     2701 catggaacac gtgagccgcc tgtatcgcga cctcatgggc aagatcaacc aggccgctgc
     2761 ggccgaagcc gatgaagttt acttatcggt ctgcggtatt acgacggaac tgaagcatca
     2821 gaccgtctgc ctgccggagg tgaagtcatg aaggcctttc tcattgccct gcagttctta
     2881 tcgcgcatcc acctggcttc gcagagcgtc tggcgcgatg aggatttcgg ccggtccgtc
     2941 ctgttctttc ccctcgtcgg cttgatcatc ggcctgcttt tggcggcgct ctattgggcg
     3001 tcgtctttcc tttttgacgc ctttggccgg tcggccctgg tcgtcgcctt ctggttctac
     3061 ctgaccggcg gcctccacgc cgacggctat atggatacgg ctgatggcct cttttccggc
     3121 cgcagccgcg agcggatgct ggaaatcctc aaggacagcc gcgtcggcgc cggcggtgtg
     3181 acggctttcg tcttcctggt cctcttgaag gtagccttcc tcagtcagct ggcaccggaa
     3241 gcagccctgc cggccctcgt cggtatcccg gcagcggccc gcttcgggac tttgatcagc
     3301 atcttccaat ttccctatgc ccgcgagcag gggctgggcc gggccttcgt cacttacgca
     3361 ccggctcata ctgtagaaaa agccctggtc ctggccctgg tgccggtctt tttctgcggg
     3421 cccttttatc tggccctctt aggtgcggcc atgctcctct ctttgtgggc caacagccgc
     3481 atcagccgca agctgggcgg cgtcacgggc gatacgtatg gcgccgtcct cgagggcagc
     3541 gaaatggccc tgctcctgat gacggccgtc ggtagcaagc tggccgattt cttgttataa
     3601 tgggacggca cgaatgttga attacttata gggaacttac ggaggattac gatgataaaa
     3661 ttatatttag cacgtcatgg tgaaacagaa ggcaatgtcc agcagtggta tcaggggtcg
     3721 accgacgtcc ccctcaatga acacggcctg gaacaggcca ggtgcctggg tgaatttttc
     3781 cgccatgtcc atctcgatgc cgtctacagc agcacgctcc agcgcgccaa ggtgacggcc
     3841 gaatgtgtcg ctgcgccgca tcatctggat gtcgtcgctt acgatgaatt gaaggaagcc
     3901 gatttcggcg tctgggaagg ccatacgtat caggaaatca cgactcagtg gccaggcgaa
     3961 ctggaagcgg tctatgcgtc ggacgggacc ttgccggccc ggggcggcga atcgttctgc
     4021 caggtccggg accggacatt gaagaagacg cgggaaatcc tgtcgcatca caaggacggc
     4081 gacagcgtct tcatggtatc ccacggggcg gccatccgct gcctgctctt cggcctgctg
     4141 ggcctcgata tgaagcgcat ctggtgcttc cagcagttca atacagcttt taacatcatc
     4201 gaatattacg gcgaccgcaa tgtcatgacc ctcatgaact gcacgcagca tctggaaggc
     4261 ctgacggggt accagcccca gtgggacaag atgccgtcgt tataaggagg catcatggca
     4321 aagacaatgc gcctcgacaa gctcctgggc catacgggct gggggacgcg gcgggaactc
     4381 aaggaattgt gcaaaggcgg gcacgtgact gtcaatggcg ccgtctgccg cgacagcagc
     4441 cagaaaatcg acccggcagc cgatgccgtt gccgtcgatg gtcaggccgt ccattataaa
     4501 gagtctattt atctcatgct ctacaagccg gccggcgtcg tatcggctac ggaagacaac
     4561 gtgtcgccga cggtcatcgg cctcttgccg cgccagtacc agggggccgg cctctttccc
     4621 gtaggccgcc tcgacaagga tacgacgggc ctcctgctca tcaccaatga cgggacctgg
     4681 gctcatggca ttacgtcacc gaagaagcat accgataaga tatacgacgc cgtcgtcgaa
     4741 ggggatatcc ctgaagatgt aggggaccgc tttgccagcg gcatcgtcct cgacgatggc
     4801 ctgcactgcc tgccggccaa agctgtccag accggccccc ggtccctgac ggtcgtcgtc
     4861 caggaaggga agttccatca ggtcaagcgc atgtgtgccg ccgtcggcct gaaagtcgtc
     4921 cagctgcaca gccgctccat cggcagcgtc gtcctcgatg accgtttgga gccaggccag
     4981 ttccggcccc tgaccgatga agaacggg
//
LOCUS       DAWCYS010000104         4888 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_973_length_4888_cov_8.877095, whole genome shotgun sequence.
ACCESSION   DAWCYS010000104 DAWCYS010000000
VERSION     DAWCYS010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_4888_cov_8.877095"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1140 a         1491 c         1165 g         1092 t
ORIGIN      
        1 ccatgacctt atagaggcga cggatgacgt cgagtatttg actggagata tgggaataaa
       61 aattaagtca gcagaagaca tcataatgtt tttccgggaa atggtatttc aggacatcgc
      121 ctgtcgtaaa agtgacattg gccaggtgag aaaatttctc tttggccaca tcgatcatct
      181 gctgggaata atcgaagccc tcgacggtcc catcatcacc ggcagccgcc gacaaatacg
      241 gcaggagcac gcctgtcccg cagccggcat cgagtatgac cgaaccgggc cgtatccctg
      301 ccagctccat gaaagacgac aagattttgg cgtcggcctt gcggtcgcgg tcccagttcc
      361 tggcataatt ttcaaaaaat atatgttcct tatccatagg gattcccctt tttgatttta
      421 tagtaagcag tagctcgtgg ttcgtggctc gtggttcgtg gcccctacga aacacgaact
      481 gctaaaaaag agactgtcgc acaaaagctg ttgctatcat gcgtggtccc tatctcttta
      541 tagttcggag tttgtagttt gcagagaaaa gctttaaatt taaagccatc cgctaaccac
      601 taacaactag ccactaatca cttaaaaggg cttgtcgtca tacgacaagc cctttttaca
      661 tggtgcggtt ggggggactt gaacccccat gaactttcgt tcactacccc ctcaaagtag
      721 cgcgtctgcc attccgccac aaccgcatat aaaattgtta aaaaaagaat atggcgaccc
      781 cgatcagatt tgaactgacg atcttcgccg tgacagggcg acatgttaaa ccgctacacc
      841 acggggccgt ttctattcag cgctcctctt gtgagaagct atggtgcggt tggggggact
      901 tgaaccccca tgagctttcg ctcactaccc cctcaaagta gcgcgtctgc cattccgcca
      961 caaccgcatg ataggtaatt gatgttggtg ctcagggacg gaattgaacc gccgacacgg
     1021 ggattttcag tcccctgctc taccgactga gctacctgag caaaaaaagt ggcgaccccg
     1081 atcagatttg aactgacgat cttcgccgtg acagggcgac atgttaaacc gctacaccac
     1141 ggggccgttt caccattgta tcagcacgga gtgcctcaac gaaaactatt ctaccatgac
     1201 acggcatttt tggcaagtct ttttttatct ttttttccaa taattccgaa aatccctcta
     1261 tgttatcggc tacatttgat gtataatata gccggtatgc tttgtttaca ccgtttttac
     1321 aaagagatta agagaggata gaacaaacaa catggttcat gcaacgaagg caatcagcaa
     1381 atgtcgtcat cggctcctgc cgctgcttat catcttcgcc gtagtctacg gcttctgctt
     1441 tgaatggcag tttctcgtct ccctgggcat gggtcccgac aatatcgaag acatgacgtt
     1501 cggcctgctg cccggcgtcg gtgctgtcgt cctgtccctg gccctgtact ggaagcactt
     1561 gtcaccaggc gccatcatcc cgccggccct catcggcctg ggctggatca ttacgggccc
     1621 ttacttgagt tacatttccc tgatcaatac gaatacgatt tacttgaaca atatatatga
     1681 tatatatgta ggactatact tttttgctat ctttttctgc ctgaacatga ttgtccggca
     1741 gtatctcccc cacaaaatcg gggccgtctt catgaccctc gtccagttcg cagcgttctt
     1801 catcattttg ctgcagtggg tctactattt cctctacaat tcgtcgatca ccacgtccgg
     1861 cgccctgctc atcttccaga cgggaccggc agaaacgctg gaatacttcc actccctggg
     1921 tctgttcaag gtagccgcca tcgccgtcgc cctggccctg atcctgggag ccctgttcct
     1981 cttgaactat cgccagaaca tcctgccccg gacacacgtc taccgcaaga tcatccccct
     2041 ggcttcgctg ctgctcatca tcgccccgtc tgtcggcgcc ttgagcgaag aaatcttccc
     2101 ggaagccttc ccgatccgga ccttcatcga cacgcacgac tacatgcagc gctcggcttt
     2161 atatgccgaa aaccacgacg aaaaatacaa tgccctccag gccgtccagc tccatccggc
     2221 cgactatccc aacacggtca tcgtcgtcat cggcgaatcg gaaacgcgga cgctgatgaa
     2281 tgcctacaat ccggaccacg tgcccaatac accgtggctg acggcgatga aatcgaatcc
     2341 ggacttcacg ctgttcacca acgcctacag ctgcgtctgg tacacggtcc ccgtcctgga
     2401 acacgccctg acggaatcga atttctacaa cgataagcct ttcaacgaat ccatttccat
     2461 catcgacatg gccaagaagg ccggctataa gacctactgg ttcagcaacc agggctccgt
     2521 cggcgtcgcc gatacaccga ttacgctggt tgccaacaca gccgacgtct ccgaatgggt
     2581 cgaccgggac ctgaaagaat cgaccctgga cggcgcgctc ctgcagttcc tcaaacgcgt
     2641 cgatccgaag gaaaagaact tcgtcgtcct ccacgtcatg ggcagccaca tcgaataccg
     2701 caaccgctac ccgaaagagt tccagaaatt caacgacggc aagattaacc aggaagccga
     2761 cttcgacaac accgtcctct atacggactg ggtcctgtcg cagatttacg actacgcccg
     2821 ggataacctc aacctcgacg ccatgatcta cttctccgat cacggcagcg atcccgacgt
     2881 cgcccgccag ccagacagtg ccagcttcaa agtcctgcgc atccccatgt tctgctactt
     2941 gtctgaaggc taccagaagc gcaatcccga cgtcgtcaaa gccatcaagg acaataagga
     3001 caagtatttc accaacgacc tggaatacga attcatctgc ggcgtcctca acatggaatc
     3061 gccgaactac gacccgacca tgtccatcgc ctcgtccaaa tggagcatgg aacggaaaga
     3121 cctgaagacc cgcttcggca agaccagcct catggaagac acggaatact agtaggggcc
     3181 ggcctgttaa ggcggcccgc agttcgcagg ccgcaaaggg cgtcccgctt gcgggaatcc
     3241 cctgaaccgc tttcacaggc cgctgtcgta cgaaggtttc tgccatcatg catgattcct
     3301 ttctctttaa tgtttgatgt atgatgtttg aagcgatagc tttaaattta aagccatctg
     3361 ctaacaacta actgctagcc gctaaccact ttaaagggct tgtcgtcgtg cgacaagccc
     3421 tttagtcaaa cctccctcat aatggtataa tagacatata ccattatgag ggaggttttt
     3481 ttatggaaca tgcttgtgtc gcagcggggt tatttcccca tccgccgatt atgctgccgg
     3541 aaatcggcgg cgatgaactg cgaaagatag acgccacggt ccgggccgta caggcggctg
     3601 cccgcttgat catcagccag aaaccggaaa cattggtcat catgtcgcct cataactacg
     3661 tcttccccga cggggcaacg ctcgtggaaa cgccccgcct ctacggcaac ctcgacgctt
     3721 tcggctatcc ggagctggcc atggatgtcc gcaccgatat ggacctggcc gaagaaatct
     3781 ttgaaacagc cacgcccaag accgacgtat accgactcga tgccgcctgg agcgaccgct
     3841 tcggccatcc cctctacgtc gaccagggca ctttcgtccc cttatattac cttcgccagg
     3901 ccggcttcac gggccaggtc gtcctcatgg cgccgcgctt cgacgactat gacagcatga
     3961 gcctcttagg cgatctcgtc gtccaggccg ctgcccgact gggccggcgc atcgccatca
     4021 tcgcatcggg cgacttgtcg caccgtctcc tgcccggttc gccgaacggc tacacgccga
     4081 aaggggccat cttcgacaag accgtcatgg aggccctgca aaaacaggac ccggccatct
     4141 taaagactat gagccgcagc ctcatcgacg aagccggcat gtgcggcctg ccgtcggtgt
     4201 atttcctctt cggcgccctg cgccatttcc gcccggtcat gccgatttat tcctatgaag
     4261 ggcccttcgg cgtcggctat ggcgtcgccc tgtacctgcc ggaaggccag gaaaaacggg
     4321 ctgaaaaacc ggctgtcgcc gacatccgcg tccgcctggc ccgggaaagc atctcgtact
     4381 acctgacgca ccatgccttc atgaacgtac ccaaatacct gcctgaagac ctgcaggaca
     4441 aggccggcgc cttcgtgtcc ctccacaagg gaagccgcct gcgcggctgc atcggcacct
     4501 tcctgcccat gcagatgaat attgccagtg aaatcatcca caacgccgta tcggctgcga
     4561 cgcgggaccc gcgcttctac ccggtcagcc tggacgaact gaaagacatc gatatttccg
     4621 tcgacgtcct gggccagccc gaagccgtag cttcaccagc cgacctggat tccaagaagt
     4681 acggcgtcat cgtcatgagc catgcccaga cgggcctctt actgcccgac ctggaaggcg
     4741 tcgacacggt ccagcagcaa atcgccattg ccaaggaaaa agccggcatc ccgcctcagg
     4801 tccagcctga cctctaccgc ttcaccgtca cgcgctataa gtaggtgaga acattgaagc
     4861 agcttattat gaaatcgacc cctccgga
//
LOCUS       DAWCYS010000105         4826 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_983_length_4826_cov_6.176483, whole genome shotgun sequence.
ACCESSION   DAWCYS010000105 DAWCYS010000000
VERSION     DAWCYS010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_4826_cov_6.176483"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1009 a         1356 c         1357 g         1104 t
ORIGIN      
        1 ttgccgtttc cgacatgaaa gacaagccgt ttgttcgcgc gtacacgtct tgtctttcat
       61 gtcggaaacg gcaaaatggg tgaacatgcc gcggatttcg atattcggca gggctgcgat
      121 ggtcttgatt tccgagacgc tgaattcatt gacctggaat ccgacgcggc tcatgcccgt
      181 atcgacggcg atatggacgc gcagggtctt gcccaggcgc cgggcttcct gggagaagaa
      241 ttcggcgttc ttcaagttga agacagccgg ttcgatgtca tacgtcagga gttcttctgt
      301 ccgccgggcg tcggtatgcc cgaggatgaa gatttcgttg tcgatatgcg catagcggcg
      361 gagctcgacg gcttcgtcca ggctggacac ggcaaagcag tcggcgccgt tttcggcaaa
      421 ggtcttggcc agttcgacac tgccgtggcc gtaggcattg gccttgacga cggcggcgat
      481 cttaacatcc ggcccgacgt gcttgcgtat ctggcgcata ttatgagccg ctgcgtcgag
      541 gtcgatctgg gcccagacgg cacggcgtgt aaaattcatg gtactttcat atgcattcat
      601 cattatctcg ccttcccatt tggaatgtta ctatatttca taatcatagc acagccctta
      661 taggccgtca atcaatcggc acaggccgtc catgacattt tcttcccaaa tatagttgca
      721 gctgaagcgg ccggcactgc gcaggtcgtc atcttcggcc agggcctcgc cattcatgac
      781 gtaggcaaag ccgctgcccg tcccgagccg gacgaagagg cccgacaaga ggccaaaggc
      841 ctggcccgtg tggccccata aatcgacggc cttcgtgcgg caacagcggg ccggccccgt
      901 gccgtcgacg aagaattcgc ccaggccata ggtgagcagg gtctggccac acgtatcgcc
      961 attgtggccg tcatagaccc actgagggct gaacatggcc tgcagcgacg acggcgccag
     1021 gaccggccgg tcctgatagc ggccgccctg aatcatcatg gccaggacgt ggcccagttc
     1081 ctccagagac aggcgcaggc cgccttgcgg tgaaaagatt gtcgcattgg tcccaggccg
     1141 gtacgcggcc aggtcgtaag tctggcagac gtcctgggca tagggattct gcaaggccag
     1201 ggcttcgcgc tccggctgga cgccgccaaa atcgtcgata gtcccctgcc aggggccctg
     1261 ttcatcccag cggccctgac tatcccgttt gagatagacc gtccccaatt tggcgaattc
     1321 agccgcttcc agattgcccg gcacatagtc ggcccggcag tccaggacgg ccaggatatg
     1381 cgctttctga tacctatcga agcgctggcc cgtcacggct tcgatgaccg tccccaaaag
     1441 gccgtaattg aggttacaat aagtaaaata aatacccggt gcctgcccgg ccggggcaaa
     1501 atgggcgccg tcttcccaat agggactacc tggcgtaaag aaggccttga cacggaccga
     1561 cggcggaatg ctgtaaaccc tgccgtcgcg caggcccgac gtatgcgaag ccagcatacg
     1621 aagcgtaatg ggcacgtcag ggtaagacgg atggcgcagg cgaaagccca ggtactggct
     1681 gacatcgtcg tccagggaca gcttccccgc ttcggcgagc tccatgaggg taaagaccgt
     1741 gaacatcttg gagacagatg ccatgcggaa acggctgtcc accgtcatcg gccaggaccg
     1801 ctccccgtcc acgcggcgga cgccggcggc aaaacgatag acctcgtgcc catcggcata
     1861 ggccaggacg gccagaccag gcacgccggt ccccgtatcg ccaataagag aggcaaaata
     1921 agcatcccag tcagttcgat tcataatagc tcctttcggg tgtggaaaag gcgctgtcta
     1981 tgaagacagc gccttttagt ggttagtggt tagtgcgtag gggccgtccc aaagggcggc
     2041 ccggctctcc tgttaggaga gctgtcagca cagctgactg agaggtcaac ctctccggcc
     2101 ctccgggcca cctcccctat taggggaggc tttcacgaaa cacgaactac aaacttttct
     2161 tacccattat aattttccgg atagatggtg atatcgtagt ggttcttgtc ccattcgtgg
     2221 gtaatctgat gcttgtacgg cgacagggcg ttgatgagga cttcataagc cttggtgccc
     2281 tgatagatgc cgtcgtcgga gatgtcgact ttatagccat tttcgtggga atagatgccg
     2341 ctggcatggt agccgctttc cgcaccgccg gtgatgacga aggaaatgcc agcctgacgg
     2401 gcttcggtat tcagatgatc caggaaagcg atggtcgagc tctggaaatg agccgtatcg
     2461 gtcgtccagt tgctgtccca cggcgtatcc ataccttccg tgctgctcca tttgcgcagg
     2521 ttgctcttgc cgaaatcacg gcggtatgcg taggcatcgg caatagctgc atcataggag
     2581 tcgtgctgat actgatactg gtattggtat tggtcttcat atttctgacg gttccaggcc
     2641 gtaaccggtg tcgccatgtg ctgcgattca gcataatggt acttgaccgt atccggcgta
     2701 atcgtaccgg gattatcaat attggcaaaa gccgaggccg gaatcagggc atggacgcga
     2761 tcccgaaggc ggtcaccgat tttgtcagaa ttattcgttg tggtagtgtt cgttttcttc
     2821 ttggctttgt cttgttcctg gacctgtgcc ataacggcaa cggccggaga taccgtttcc
     2881 gattccttga cctgtgctgc cgaagcagtg acagttccca taagacagca aagaaacagg
     2941 attgcgatga tagacttctt cattgaattc ccccttaaaa tactgaaatt atgtgtatga
     3001 cattcggtta ttttcacgaa cagcgtttca attgtaacat attcagggga atttgaaaag
     3061 tcctgctacg aacccaggag ctgacgctgt tccatttcct gggctgccgc ctcatcctgg
     3121 tcggccttct tcgtgtctcc taatttgcgc cagacctggc tgcgcagctt gtagccatct
     3181 tcatagggct tgacggccaa agcctgtgtc aaatactgta cggcccgcgg ataatcgccc
     3241 tggtccatgg ccagctggcc caggctgtcg tagacggcca atttatcgct gtcatccgtg
     3301 gccaggcgga ccgattggtc atagtctttt ttagccaaat cgaaacgctt caaatggcgg
     3361 taacattcgc cgcggttatt gtaatccata gccgtttcct tcaaagccag ggctttcgtc
     3421 aagtcggcaa tggccaactc gtcctggccc gtctgggcgt agaccaggcc ccgttcggaa
     3481 tagacccggc tgtcgtcggg atggcgtttc accgcttcat tgagcagtgc cagggcctgg
     3541 tcgtatagtc cggcttcttc ggcgacggaa ccggccagga agtaatcgcc gcggtgcatg
     3601 aattcataca tgacgccgat acggcggctc aggtcggcca ttttcttgtc gcccttctta
     3661 tcatagatca ggctttcgag gatatagttc tgaggaaagg ccggagccag cttttccccc
     3721 tgtttgacgt catccagggc cttgtcatac gattcgatgg ccgtataggc ccgagcccgg
     3781 ttgaaataca tcagtccctg caaaggatcg cccttctggc tcaaggccag ggccttcgta
     3841 tagtcgttga cggccagcgg gtattctttg cgctgttcgt aaatggcgcc gcggttgaaa
     3901 taatggaagg ccgtgcccgg ttccagggaa atggctttat cgcaatcggc cagggcttcg
     3961 tcattccgtc cgaggaggct gtaaatcgtg ctgcgccggt cgtaggcatc ggcatcatcg
     4021 ggatgttctg caatctggcc gttgacaatc tgaagggctt tctcataatc cttggcccgg
     4081 acagcctgca ggagttcact ttccccgtgg gtctgcaaag caagggcccc ttggccatcg
     4141 cctttcgcca gggtcacatc gtccgccagg gacaggcagg gaacggccaa aaagcaggaa
     4201 atcagcccgg ctaatatgca ctgtttcaat cggttcataa gaatccccct atctctcgaa
     4261 ttttgcttta ccttataaga ttcgacataa gattcgacat agccccgcca aattcctgcc
     4321 aaaagacaaa aagtaatatg gtataatgaa gaaaaaaaac gaatgaggtg agcaatttgt
     4381 ttgaaaaaga atatgaagcg tcagcccatg gcgccgctgc caaggtccgc ttcctggagg
     4441 aaaatcccct gggctatgtc atggtgtccc tcctgggcgg cgcttatctc ggcttcggcg
     4501 tccttctgac ctatgccatc gaaagctacc tcggaccgca gccatcggtc catatcatca
     4561 tgggcctttc tttcggcgtc gccctcagcc tggtcatcat agccggcgcg gaaatgttca
     4621 ccggtgacgc cttcatcctc tataacggcc tgcggcgcaa gaccgtgacc gccggagacg
     4681 tcgtgaagct cctggtcgtc tgttatctcg gcaactggct cggcgccctc atcctgtcgg
     4741 tcatcttcta tggcacaggc ctcgatacgg gagccatggc ccaggccctg acggaagctg
     4801 ccgccttgcg gatgaacctg ccgccg
//
LOCUS       DAWCYS010000106         4807 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_986_length_4807_cov_5.051768, whole genome shotgun sequence.
ACCESSION   DAWCYS010000106 DAWCYS010000000
VERSION     DAWCYS010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_986_length_4807_cov_5.051768"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1110 a         1483 c         1281 g          933 t
ORIGIN      
        1 tgtcaacacg ccgatgacga cgtcttcgcc ggaatcggcg atctttgaaa ccttcccgat
       61 catgctccag tcgacgcggg atttcctgac gcagatcgaa aaggccgtca acctcgacat
      121 cccggtcagc atcgtcgata tggccttttc caacggctcc gacaacaccc tggtctacgg
      181 cctctatcag gataagatga tgtaccgcct ggctgcctac aacggctgga atacggccag
      241 caactccgtc ggctacggca tcgcccaggg cgtcttgtcc aagtatatga cggccgatgc
      301 ccaccgcgac atgctgacca cgcagtatct cgacaactgg gcgtaccagg ccaatgtccg
      361 cgattacatc taccgtatgc agcagaaact ggaagccggc gtcgtcactc agtattaccc
      421 gacgctcaac gaagaactcc agagccgcac gaaggaacag ctccagcgct atgcgtcgac
      481 gtatctcggc atcgacccca agaccgtcga tgtcaccctg ccgtggcagc gcctgttcga
      541 agtctatgtc gatgtcaagc cgacgccgtc tgtccccctg gaagccgacg tgcgccacaa
      601 tatgaatgac cgcgaactcc agaacctggc caatgaagtc tcggccgccc aggcccagct
      661 cgatgcggcc cagaccgtca atgccgacgg gacggtcacg caggccgacc cggccgtcca
      721 ggcccagctc caggccgcca aggccgctgc ccaggcccgt tacgaacagg aaaaacaggc
      781 ccaggccatc aatcacagtg aagaaaaagc ccagcagagc cgtacctggg ctgcacagaa
      841 ataggatacc ccatggaaca acagaatcat acaacgacac aacttcatga gaaacgccgc
      901 gttccgcttg gaaagaaggg ccgccgtatc gctgccgccg cagccgggct ggtctgtctc
      961 ggcatcgccg ccgtggctta cggctatttc ggccccaatt tcctcctgcc ctataagagc
     1021 ggctacgtcc acgtccgcag cgacatgacg gcttcggaag tcggcgatgt cctgaccgat
     1081 gaaggctata ttgccagccc cttgtggttc cgcgccgtag cgaccgtcac gggacaggcc
     1141 ggtgacatcc atgccggcga gtataccttc gattcgcgca tgagcctcca tacgctgctg
     1201 gaaaaactaa ccagcggcaa gtcggaagcg gaccgcctgg tcgtccccga aggctatacg
     1261 gtccgtgaaa tcgccaaggc cgtcgccgcc aacgggcgca tcagcgaagc cgatttcctc
     1321 aaggctgcct cgtcatcgga ccagctcctg ccgtatatga agggcaatcg caaagtgacc
     1381 ttcccgacag aaggcttcct cttcccggac acgtatttca tcccttatga cgcgacggcc
     1441 gacgacgtcg tggccatgat gctcaagaac ttcgacgccc acttgacgga tgccatgaaa
     1501 gcgggcattg ccaagcagaa cctgtcgatc tactagttcg tcaccttggc ctcgctcatc
     1561 gaaaaggaag cgaaatacga aaaggaccgg cccctcatcg cgtcggtctt cgagaaccgc
     1621 ctgaagatcc acatgaaact ccagtccgat gccagcattt cctatgctat ggggacccac
     1681 aaggcagcct acagcatcaa cgagacgcag tacgattcgc cgtacaatac gtaccgctat
     1741 gaaggcttgc cgccgggacc gatcggcaat cccggcatgg actgcatgga agccatcctc
     1801 gaggcaccac agacgaacta cctctatttc gtcgccgata aggacggcca taattacttc
     1861 gccgccacct atgaggagca catgaagaac gtccaggagc acatgccatg atggaggcat
     1921 tgttccagga tatgaaagcc tgtgcccgcg agcggaacct gcccatcctg cgcgacagcg
     1981 agctgccctt gttcacgggc atcgcagcgt cgtgccggcc gaaacatgtc ctggaaatcg
     2041 gaacctgcat cggctattcg gccctgcaga tggcaccgtt cctggcaccg ggcgggacga
     2101 tcacgaccat cgagctcgac cgtgagcgct atgacctggc ctgccagttc ttccggcgct
     2161 caccctatga aaaggtcatt acggctatcc acggcgatgg gacggcgctg gcggaacagc
     2221 ttcccggtcc gtgggacttc gtcttcctcg acgggccgaa agggcagtat gtgcgccagc
     2281 tgcgatatct cctgcccaag ctcctgcctg gcgccatcgt cgcagcggat aatatcgact
     2341 accacgacat gttttatatc gaagggaccc tgccgcacaa acaccgcacg gccgtgaccc
     2401 gtttgcggga attcatggac ctcgtcggcg accggtccct ttttgagact gtattttttg
     2461 aaaatggcga cggcatgacc gtttcgcaat ggaaaggata agagaatggg aaaaatggaa
     2521 ttgctggctc cggccggcag tatggataag atgaagatgg cctttttata cggcgccgat
     2581 gccgtatacc tgggagggaa gtccttcggc ctgcgggctt tcagcgacaa cttttccaat
     2641 gaagaactga aggaagccgt cgactatgcc catgcccggg gcaagcacgt ccacgtgacg
     2701 gtcaacatct tcccgcataa cgacgacctc aagggcttgc cggaatacct cgtctacctg
     2761 cgcgatatcg gcgtcgacgc catcctcatc gccgacccgg gcatcttcgc cctggcccgg
     2821 cagctcgtgc ctgacctgcc tgtccacata tcgacccagg ccaacacgac caactgggca
     2881 tcgacgaaat tctggcgcga taacggcgca tcgcgcgtcg tcatggcccg cgaagtgagc
     2941 ctacgcgacg tcaaggaaat ccatgccaaa gtccccgata tcgaactgga aggcttcatc
     3001 cacggcgcca tgtgcatttc ctattccggc cgctgcctgc tgagcaatta cttcacccag
     3061 ggcgaccgcg actcgaaccg cggcgaatgt gtccagtgct gccgcttcaa gtacaacgtc
     3121 gtcgaagaga agcggccagg ccagtatttc cccgtcgtcg aagatgaacg ggggacgtat
     3181 atcttcaact ccaaggacct ctgtctcctg ccgtccctta cggccctcta cgacgcaggc
     3241 tttacgagcc tgaagatcga agggcgcctg aagcgcagcc actacgtcgc caccgtgacc
     3301 aaggtctacc gccaggcctt cgacgcctac gaagccgacc cggaacactt cacggtccgc
     3361 caggaatggc tcgacgaatt ggaaaagatt tcccatcggc cctacacgcg gggcttctcc
     3421 gtctcccggc cgacggcggc cgaccaggtc tatagccagt cgagcaatac ccagacacat
     3481 gatttcatcg gcctcgtcaa gtcctgtgat gaagaaaagg gcctggcctg ggtagaacag
     3541 cggaaccact tcaaactcgg ccagaccgta gaattcctcc agcccaaggg ccatctggtc
     3601 cgctatacca tcgaccgcat cgtcgatgaa gacgggcagg aactcgacgc cgcccgccac
     3661 gcccagcagc tcgtcggcct ggccgtaccg gaacggctgg aaccatattc catgatgcgc
     3721 cgccaggtga agagccatgt ttgatgaaga ctgggtctac ttccaggccc ttccgtcgga
     3781 aatgaccttc atcaaccgca tcatcgaagg ctgtgactac ctgggcgtgg tcacggccct
     3841 ggacggcaag aagggcatag gcttcgtacg gacgacgaag gacacggcag gcgagacgat
     3901 tgaggtactc caatcgctgc ccatcccggt gcgcatcctg agccgccagg aagccttagc
     3961 gcaagctgaa caaaagtagt gtatgaaatt tacaaaattt atagttatac gatttagtga
     4021 aaagtgctgc ccctgacggc agcgcttttt ttgtaggggc ttccacgtgg ggagcccgcg
     4081 tgaatcccgg gcatagctca tactggtcat gctgtttgtc gtccccatag tctgacgggc
     4141 gccccacgtg ggcgccccta cgatagagca attccatgcc ggttcaaaac gttacatttt
     4201 aaaagatagc agtccttgtt gtaatgtgta aaatagttgt ttctgctaat cattttttgg
     4261 cggttagccc tttaaattct tgacagaatt ggtgtataat taataatata atcataaaat
     4321 actttatagc gtatattata tgcgtatacg gtacgctatt gaatctaaga cccgccgtct
     4381 gacgacacga tgtccattat catacatact gaaaccataa gcgaagcggc cgggtcccag
     4441 tcaagtgagg aggagcttct atgaaaaaat ctaattgcct ggccatgatt ttggccggcg
     4501 ggaaaggaag ccgtttgggc gtcctgacca aagaccaggc taaaccgggc cttttgttcg
     4561 gcggaaaata tcgcctcatc gacttcccac tgagtaactg ccgcaattcc ggcttcgata
     4621 cgggcggcag cctgacccag tctcagccgc tggaactgaa ctggtacatc ggcaacggca
     4681 gctcttggaa cctcgactcg accaagggcg gcacgtacgt cctgccgccg tatcagagcg
     4741 acggcggttc caactggtac cgcggcacag ccgatgctat ttaccagacc ttgaacttcg
     4801 tggaaat
//
LOCUS       DAWCYS010000107         4807 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_985_length_4807_cov_5.084175, whole genome shotgun sequence.
ACCESSION   DAWCYS010000107 DAWCYS010000000
VERSION     DAWCYS010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_4807_cov_5.084175"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1127 a         1369 c         1311 g          990 t
ORIGIN      
        1 gtcttactgg aaaggggctg gaacgtcgcc atcagcggcc gccatcggga aaaaggggag
       61 gccgccctgg cggcgttgtc tgcgccggac cggacctggt atatccaggg cgacgtggcc
      121 tgcgacgacg actgccgccg gatggtcgaa gagacggttt ctcacttcgg cggcctcgac
      181 ggtttggtca cgtcggccgg cgtctatgaa caaggcctct tggaaaatgt cacgcctgcc
      241 gacatgcagc gnnnnnnnnn ncgacactgc agcggctcgt ttccatctat gtcttcgtga
      301 cattctctct ctgccgctat gccctgccgc acttgcggcg ccggccgggc agtatcgtca
      361 ccgtcagcag cgacgccggt ctccagggca acatcgcctg cgctctctac ggggcgacga
      421 aaggcgccgt cgtggccttc acccagtccc tggccctgga agcggcaccg caccagatac
      481 gggtcaactg cgtctgtccc ggtgacgtgg aaacgcctat gctggagcag cagctccggg
      541 accatcccga tacgacgcgg gatgccatga aagagcagta tcccttgtac cgactggccc
      601 ggcccgatga agtggccaag gccatcgcct ttctcttgag cgatgacgcg tcgttcatta
      661 ccggcgtggc acttcctgtc gatgggggtt tgacaagctg gtagaaacat gggaaaatat
      721 agagtataaa ttttaatacg ttaaagaatg gggtgtttgt tatggcagaa gaagtataca
      781 aagaagaagc caagactgta gaagaactgc aggcagaaat cgccgcattg aaacaggaac
      841 tggccgacat gaaggcccag tcccagccgg ccgacgatgc cgcagccagt gtcgtcaagg
      901 ccatggaaga gtcgacagaa gacaaagacg tgaaagtcga aatgttgtgc cgctgggctg
      961 cagcccgggc cggtgccatc gtcattgcgc cgctcgtcgg gaccgtcgcc ctcatggcca
     1021 acgaagtcta tctggtcagc cgcattgcca aggtctatga tgtcaaactg agcgaacggg
     1081 ccctcatcgc cttcctcggc gccgtcggca gccgcgtcgc cggcagcctg ctgacgacga
     1141 ttattccttt cagcgccatc caggtacctg tcgccgtcgg catcacttac agcctgggcc
     1201 gcgtcaccca gcgctggctc aaggacggca tgcctaccga catgggtccc tacgtcgaca
     1261 tgatgggcga atggaaggat aaggctcggg aacaagtcga caaactcaag gaaaatccct
     1321 tgaagaacgt ccccctcggc gatgaaacca tcgacttcat gaagaaatgg ggcggcgtcg
     1381 ccaaggaccg cttccaggac gtcaaggaaa aaggggaaga actctatcac accgtccgcc
     1441 atcacgacga cctggtcgat gaaatcgtag aagaaaaggt cgaagaaacc aatgccggtg
     1501 aagccgaaaa ggcagaatgc acgggagaaa ctgccgaaac tgtggaaaga gccgaagcca
     1561 cggctgaaac gacggcagct gccgaaccgg aaacggcagc ggctgcagac ggcgatacgg
     1621 cgtctgtcaa agacaaggtg aaagccgccg tagaagatgc caaaatccct ttggaagata
     1681 agaagtaagg tgaaggccca tgtggaaggt actgcgcttt tttgcacgtt tttatacacc
     1741 tggcaaattc gtcacctttg tccacaagac agggaggaag atccactttt tgccgaagct
     1801 cctgacgatt ttttactgta tgcaggatga agacacgccg aaattcatca aattggccct
     1861 catgggggcc ttggggtatg tcattctgcc ctttgatttc gtgcccgatg tctttgccgg
     1921 cttcggctgg ctcgatgatg ccgccgtcgt cgctgcggcc ctgcgcctgg ccgggactta
     1981 cgtcaagccg gaacaccgtg aaaaagtacg tcatatgttc ccgttcttcc gcgtccgcgt
     2041 gtgaaggcgg ggaaagccgt ctgaagggct ttgccatcat gcacggtcca tttacttatc
     2101 gtttgtagta gggagtttgt agtttgtaga agatggcttt aaatttaaaa atatttacta
     2161 aaaactacaa actgcaaact taatactcaa aaagcggctg tctttatggg acagcctctt
     2221 tttcctttga caaacatagt tcctcatcgt aaaatagtat gagtgtgcat gatgtaagat
     2281 gcatatagtt aggagttaag aacttatgat tactgtcgtt gaaaaagtgc ggttctatga
     2341 aaccgacatg atgggcattg cccaccattc caatcatatc cgctggttcg agtgcggccg
     2401 ctgtgaatat ctggcagctg ccggcatcga tttgttcgcc ctgctggacc ggggtatttc
     2461 ctatcccatc aagagcgtgt cctgcgagta catcagtccc atcaactatg ccgataccat
     2521 cgacatcgag acgcggctgg tcaagctgag ccgggcgcag atggtctttt cgtaccgcat
     2581 cgtcaaccac gatgaccacc gcctgctggc gacggggacg acgcagaacg tctttgccca
     2641 taaggacagc ggcaaagtcg cccgcctcga tgaagccgat tataagaaat tacaggccat
     2701 gtatgccgaa gataaagcgg ctgcccaggc cgggggaggg gtccatggaa cgtaatcaac
     2761 agcctatcgg cgtctttgat tccggcgtcg gcggcctgac cgtcgtcgcc aagctgcgga
     2821 tgctgctgcc tcacgaaaac atcatctacg tgggcgatac caagcgcaat ccctatggga
     2881 cgcggacgcc ggaagaaatc ctgacctata cgcgggatat cctgcgcttc atgacggccc
     2941 agggcgtcaa gctcgtcgcc atcggctgca acaccgtgac ggccgtcgcc tatgatacgg
     3001 tcgtccagga agtaccgtat cccctcatcg gcatgagccg gggcctgcgg acagcgcggg
     3061 aaatcagcgc gaccaagaag attgccgtct ttgccacgcc gctcacgatt gccaaccaca
     3121 gccatcgcaa agtcgccgcc gccatggacc cggacatcga aatcgtcgag cagtcctgcc
     3181 cggccctggc caatctcatc gaacggggcg tcctcgacgg cccggaaatc cgcgagcctt
     3241 tgaagcgcta tacggaaccg gtccttcatt ccggtgccga tacgggcatc ttcggctgta
     3301 cccatttccc cttcgtccag cccttgtttg aagaactctg cggtgattcc atcgcttttg
     3361 tcgacccggc tcatgaaatg gccctggaaa cattggacgt cctgaaaaag gatggcctcc
     3421 tcaacaagca gccggaaccg gggactctcc gcctgtgctt taccgcagag agcggccgcg
     3481 gctcccgcct ggcccagcac ctcatcccgg ccaatgaatt tacagctgaa gaaatttccc
     3541 tcacttaatg gttatcaaaa tattatataa aaggagtctt gcgtcgtgtt tttctatgtc
     3601 ttactcaatg ttgtcattgc cgtcatcggc attgtcgctg tcttgtacct tttattccgc
     3661 ctctttgctt ggcggcaggg cgatgcccgc ttcgtcatcg aagcccgtcg ccgcaaaccg
     3721 tttgaactga aacagatgac ccaggatacg gctgtctttg aaacggaagt ccccctccac
     3781 aacgccggcc gtcagctcgg tacgatcatg gacttctatc cccggaccct gctgccccgc
     3841 gaacagtacg acaaggtcat cgtccattcc cagctggcca ataaagcggc tgaacgcgat
     3901 gacaactatt gggaatcggt catcttcaac cctggcgaca agggcatgat ccgcgtcact
     3961 atcgccctgg tcagcaaaga cggcaatatc cgcgaagacc tgaagacctt ccctgacatg
     4021 cccatcgatt tggtatacca ggtcgtttcc cgcagcgaat ggtacatcca taaagcccgc
     4081 atcaccctga aggcttcgga agtacagcac gcattggaac agaactagga ggttttaacg
     4141 agatggcaga attagaatta gtacctgtaa aaacgagaat ccttacggat aaagatgata
     4201 ttgtcgacgt cattgaagaa tatgcaaaag atatcgtcgg cccggacgat ttggtctgct
     4261 cggccgaaag cgtcgtcgcc attacccagc gccgctatac ccgtccggaa gaactgacgc
     4321 cgtcgtggca ggcccgcctc atgcgccgct tcgtcccggg cgaaggcagt atggccagcc
     4381 tctatggcat gcaggctgcg atgaacctcg aaggcgaatg gaagatgatg ttctggttct
     4441 tcatcggctt cttggccaag ctcgtcggca agaacggtgt atggtatgcc aaatgccgcc
     4501 aggcatcgct cacggacgat gtcaccggca ccatgccgcc ttttgataaa tgcatcgtct
     4561 acggcccggc cgaaccggat aaagtcgctg aaaagatcaa acagcgcatg ggctgctacg
     4621 gcgccgtcgt cgctgacgtc aacgacctca agcgctcggc tgtcctcggc cacagcaaag
     4681 gcctgaacac gaaggaaatc gccaaaatcc tcatcgacaa ccccttcggc aatgcttctc
     4741 agaagacacc tatcgtcgtt atcaagaatt acgcccagtt cgtcaagaac cggaagaagg
     4801 actaagg
//
LOCUS       DAWCYS010000108         4797 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_989_length_4797_cov_6.208773, whole genome shotgun sequence.
ACCESSION   DAWCYS010000108 DAWCYS010000000
VERSION     DAWCYS010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_989_length_4797_cov_6.208773"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1126 a         1341 c         1279 g         1051 t
ORIGIN      
        1 atataatatg ataagatgtc tatttttcgt ctttcttctg ccagaggcgg ctgccaaaga
       61 gacccgctgc cagcagacag accatgatac cggctacgat gaggccgtgc ggattgaagt
      121 ggatgaggtc gtaacccagc cagacttcca tacagacggc caggaacttg ccggtcatcg
      181 tcgccgccgc atgttcgccg aagcggacac ggctcagggc acaaagggcg gtgacgacga
      241 aattcggaga atagggaatg gcccggaaga aaaagataag ccggaaatcg ataccgcggt
      301 tcagcttgga cagccagcgg ctcttatcga ccaggacctg tgccttgcgc cggaagacat
      361 agcgcatgat gacaaaggcc aggatattgc cgatgacctg accagcccag gaaatgagga
      421 tgcccggcag gaggccgaac aagatgccat tgaccgtgac gaactggacc gacggcaggc
      481 cggtcatgtt ggtcaggacg atgagcagga tcatgaggac cacggcaata ctgccaaaag
      541 aagaaatata cgacgtcagc cccggaatat catgactcac cgtcagctgc cataacgtgc
      601 tgtaaaacga aggaatggcc cagtggatcg ccaaaaacaa ggccacgatc agccccagcg
      661 tcaaaggctt catcaaattc gcaatcatcg atcgattcat cattgtctca ctttcttgaa
      721 aagtactcat aaatccaagg tgaccccaac gggccgcctt tcaggacggc ccctacggcc
      781 cactagccac tagccactag ccactagcca ctagccacta gtcactagtc actagtcact
      841 agtcactagt cactagtcac tagcctatta taccatattg cgcgcaaaaa gggcttgccg
      901 catgacgaca acccctttta agttgttagt ggttagcgaa ggattttaaa ttaaaaaaca
      961 aaagggaccg agcccgatgg cagaaacctt cgtgcgatac cctgcggcct gcggatgtcc
     1021 tgcaggcgtc ctttctatat gatgatgccg tccaggtggt cacattcgtg ctggatgatc
     1081 tgggccgtcc agccggtaaa ggtctggcgg tgtttgcgga agcggcggtc ctggtagaac
     1141 acctgtatct tccggtagcg cgttgtcttc cgcgtgccgt ccagggacag acagccttct
     1201 tcggcctcaa aaggatcggc cttcttttcg atgaccgggt tgatgaaaat ttcgtcgccc
     1261 ctatccgtat ggacgacgat gatgcgcttc aaagaaccaa tcatattggc cgccatgccg
     1321 acacaggtca tgcggttggc cttcagcgtg tcgagcaggt cctggacgac gggcaaatca
     1381 tctttcgtcg ccgcttcgga cttacggccc aagaagatga cgtcatgtac aatattgcga
     1441 atcacaatgt tttcctttcc tagggatata agaccaagta aatatccatg tcatgatttt
     1501 tcaagaaagc cgctgccgct tcccgggcga catccaaggc ttctgctttc gggtagccaa
     1561 agatgcctgc cgaaatgagg ggaaaggcgc tggaccggca gccgtgcgac gctgccagtt
     1621 ccagggccgt ggcataacag ctggccagca ggttccgttc tcccctttgg ccgccctgcc
     1681 agatgggacc gacggtgtgg atgatgtatt tcgccttcaa tccgtaactg tccgtcaata
     1741 ccgactgccc cgtcgggcag ggcgcctgga ccaggcaggc ttcctggagt tcccgggccc
     1801 gcggcccgac agcgtggaaa atggccccgc agacaccgct gccggcgaga aggccagaat
     1861 tggctgcatt gacgatagca tccgttccca tttggacaat atcgccgtga acgatagaaa
     1921 atgccatgaa aatccctccc tgagtgaaaa ggccaatact tacctctacg taaggacctg
     1981 acccgaaagt gccttcttcc ataactgaga aagattattt ataataaggt catcaatcaa
     2041 ggaggtcctt ttacatgaac aaaaaatcaa tccttttagg cctgaccctg gcccttgtcg
     2101 gctgcctgcc catggggaca tcgctggcag ccgatacgcc ccatttcaaa agcaacaaag
     2161 ccgcagctga atatttctgg aatgaagtat ttaacaaaca cgacacagcc gtcatcgaca
     2221 cgatggtcgg tcccaaatac aaacagcaca gcccggaatt cgccgatggc aaacaggcct
     2281 ttaaagatgg cgtcacgggc ttcctgaaag cctatccgga cagctcggcc gtcatcaagc
     2341 acatcggcgc cgatggcgac ctggtcttca tccacaatca catcaaactc aataaagcag
     2401 accgtggtca ggctgctgtc gacattttcc gcatcaaaga cggcaaaatc gtagaacact
     2461 gggatgtcat ccaggatatc ccggaaaaag cagccaacga caatacgatg ttctaacgct
     2521 tccccaaaga gaggctgtcg cacgaagaca gtctcttttt attttccaaa aaagccatcg
     2581 acccagccgt tgatgccctg catgacatgg atcagctttt cccctctgtc ggtcaggctg
     2641 tattcgacag tcggcggtac cgtggcatgg acgttccgcc ggaccaggcc gttgcgatcg
     2701 aagacgtgga ggttctccgt cagcactttg gcactgatgc ccggcaggca ccgctgcagc
     2761 ttggcgaaga acaccggccc atctgccagg gccagtaaga tagacgtccg ccatttgcct
     2821 ttgatgatgt cgatgaaaca atcgaagggg cagtcatagg ccttgccatc aaacgtatac
     2881 ataaaagcct tcccttctaa taatcttcgt cccaagcgtt gatgacgaca gggtcttttt
     2941 ccagcatggc taattgggcc gggctgaaat gacggcggtc gatgacggct tcgtagacga
     3001 attcctggaa ataagcttca gagcagacga aatagccttt gtcgccgaca tccttgcccc
     3061 aggaattttc gattttccaa cggtccggct tgccttcctt gtcgaaatgg acgcctgtga
     3121 gcaacatggc gtgagtcgcc gaactactgt tatactccag ccgtttgccc ttgggcatgg
     3181 ccgtcgaaaa gccgcccaaa agggcttcgt actggacgct ggccgggtcc cagatacctt
     3241 ccttgcgggc gccgaaggcc ccggcatcgc aggcgaacca gatggcctga ccgtttttca
     3301 actgtttgac gcagagttct tccagggctt cctgcgacag gttcagggcc ttcatgtcgc
     3361 agccggccat attttcgacg gcatggaact ggatgggcgt atccagcggc ttatctgccg
     3421 tcggttcatt gatgacggcg acataatcgc gcagggacag gccgacgtat ttctgataga
     3481 aagcctgcgg cgtcaggtcc cgctcttcat ggtagttgcc gtctttatcg cggtaggcga
     3541 agtcaaaggt ctggacaggc tggccaaagg cgatgcaggc agccttgtag acttcggcca
     3601 tcatggccgc cttgcgcgga tacgggtccg caccgtcctg gacgagttcc cgcaactcca
     3661 tggcatcttt gcgcaggagg cggttcaatg tcgctacaaa gcggtctatg tggttggact
     3721 gatacgtttc cggctggacc tgtttgggca cgacgccgta tttttcgatg aggtccacgg
     3781 cttcgctcca atagccgccg tcagtcatgc cccgcaggac gtactgcatg agctggcctt
     3841 ttatgggttc ctgggcgttg tcgatgacca tttccaggaa attgttggct ttttccaatt
     3901 tatcgtaaaa agacaagtaa ttctgggaca gctcgaactg gtcgacgtgg cacgtttcgg
     3961 ccaccttttc ccggaggata ttcaaagacg caaagagcca gcaacggcct gatttctgct
     4021 gggccgtaat gccccgcgtc ttcacttcga ccgaaaaagc gcctttaagg cgggcaccgg
     4081 ccacggggac ataagccagg tcagccatgt ccgtcttggc cagggccgaa tgaaggacct
     4141 gggcctgccc gtcggcttca taagccgtgc ggaactgggc taacatatca tgagtaatct
     4201 ctttcatcaa aattccccct tttatcattc cccatcctga gaatgacgag aatcgccaca
     4261 tcgcctgaac cgctccgtag gggacgtcct gctaaggcgt cccgctgtga atcttggata
     4321 taactattca ggtatatgag gaatgttttc acacatcacc cgttatgcat tacgtataca
     4381 ccatcatatc aggcgggccg ccctttggga cggcccctac gaaggtcttg tcttctcaca
     4441 ggttgttcat cgttgcagga ccatccggcg cctacggacg tttagctgcc cgcttggcga
     4501 tttcgatgac cgtttcgcag gccttgacca gcgacggaat gggcaggtat tcatagcggc
     4561 cgtggaagtt ggcgccgcca gtgaacaggt tcgggcacgg caggccgcgg aaagagagct
     4621 gggccccgtc cgtaccgccg cggataggag cgacctgcgg cgtgacgccg actgcttcca
     4681 tggcttcttt ggccaagtcg acgacatcca tatggccgtg ttcgattttg tccagcatct
     4741 gagaatagac atcatacggc gtgacgacaa cggtcccatc gccgtatttt tcattga
//
LOCUS       DAWCYS010000109         4789 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_993_length_4789_cov_5.247360, whole genome shotgun sequence.
ACCESSION   DAWCYS010000109 DAWCYS010000000
VERSION     DAWCYS010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_4789_cov_5.247360"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1796..1805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1281 a         1286 c         1188 g         1024 t
ORIGIN      
        1 acacacagcc agcggccttg tgcgtagggg ccggcccaag cctttttggc ccgtcaggtg
       61 ccgaccgaca gaaggcgcag gacggttgcc aaaacggaca gctgtggctg ggcggcccgc
      121 tgtgagattt gggcataacc tattctggat gatggtaggc tttttcaaac cacgagtcat
      181 tcgttatgct aaaaggctgt acacaggatt cacgacggcc cggctctcct cataggagag
      241 ctgtcagcga agctgactga gaggttgctt tttttacata gcttgtagta cggagtttgt
      301 agtttgtaga agctggctta aaatttttac ccgttgctag ctactagcca ctaaccacta
      361 aaaaaaactt gtcgcgcggc gacaagtttt ttttatgcta agacacattc tgtcctctta
      421 ttgatgtata ataatatgtc caaggaggcg ataggaatga aacagattga cgtagtacaa
      481 gcacagcaag atatccagtt catctataac tgctataaga aatttgccct tgacgtggac
      541 gaatgtcggg cggtccttaa agaacgcgac tgctgcgaac atcgcgacag tgatggtcat
      601 ctgctctttg aggatgccag ctccctgacg atgatagata gcgccaagaa ttgtcatgat
      661 acgtatatct gcaaaaaatg ccatactgtt ttttcgctga aaattgaccc ggatacgcac
      721 gaatacagct acaatgaagt ctttagatat aacagcctgg atgaccttct caagccggat
      781 ttcatagcac acctcgaaaa atccaatcat attttggaca agatgcagca gttcacgccc
      841 aaagatgtca cctttttcca caataacctt aaaatccagg ccgaactgct ccagcgtgaa
      901 atccgtcgac aaacggctat cttagagaaa ttggattaca tcaccctgga cctgccctat
      961 tacgatgact tttcagccaa acacatccgc agcatcctct ttcccctgta ccagcagatg
     1021 acattcagga cattttgggt actgagcttt tccgtgccca tctttaatac tctcgattat
     1081 tatgattgta cggtcaatct cctcgacgac atcccggggc tcaatatgga cgaagcgctc
     1141 atgcttatcg aagactgctt caaaaaggcc ggcacgacct tgcaggaaag ccggcgccgt
     1201 tatgcctggg agcccgtctg taatcaccgc gatgaacagg ggaacctgct gatccataat
     1261 ctgctgaata tggtacagac cgttgaatgt gaacgctgcc atgccgtctt taccctgaat
     1321 ttcaacgaac aactgggaaa atacacccat ggctacctcc tgaacgactg ccacttctac
     1381 gacttcgaac gggctggcgt cgccgagaaa gcagaagccg tcctggaaaa agacagactt
     1441 tccaacgtcc attcgctgac ggactattac catatgcgcg aagaattgaa agaacagtac
     1501 caggccgtca cggaagataa gatggcctgc tgggaactgg tgggacgcct gaatacatgc
     1561 ctcttagaaa tcgatggcct gaataccttc acgcgccatc aagtcgaaga cagcgtgttg
     1621 tatgcccaaa aagaaatgat cgaccgttat ctctggcaac atggacttca aaaacagctc
     1681 aaagcccggc tgaaagaatt cggcaccctt ccgtccctga agtatccctg gtataataag
     1741 gagaacacga agacagaaga tacagagaaa ggaaagatac tgcatggcac gttatnnnnn
     1801 nnnnnctcgt tcataaccca ccaacgctga cgattttcag ggccgcgaag gcgaaataca
     1861 ggtctatcag gccttacaac aattaagtga cggatatgtc gtcttccatt ccttccgctg
     1921 gctcggcgaa ccgggccaat accggagtga aggggaaggc gactttatca ttttccaccc
     1981 gaccaagggc atcttgtcca tcgaagtcaa atccggcggt atcggctatg aagaaggcag
     2041 ttggatccaa atcaaccgca ggaataacag caaaaaacgc atcaatccct taggacaggc
     2101 tgcggaaacg cagtaccgta tccagcggat cctgcgccag catttcccct atgactgtcc
     2161 catgattggc cgggcggcct ggttcacgtc ggtcgccatg gattcgaaac tgcgtctgcc
     2221 agccgaagcc gttccggaaa tcatcctgga ccagctgagt ttagcacatc cggaagaagc
     2281 cctggacaag gtttatgcct attggaaaga ccacctgggc cggtccttta caccgttgaa
     2341 gccgccggaa ttcaaagccc tcatccagac actcatgccg tctttccgca ttgccgaaac
     2401 gatttccagc agcagccggg aaacgaagac gtcctacgtc cagctgacga accagcaatt
     2461 ctctataccc cggttcttgc aggaacagcg gacggcggcc atttacgggc ccggccggca
     2521 cgggcaagac tatgctggcc gtagaaaaag cgcggatgct ggccagtcag ggaaaaaagg
     2581 ccctctatct gtgtttcaac gaattcctcc tgcagtacct gcgacgccat tatcacgatc
     2641 cgcagattac cttccataat gtacgcactt tggctgaaga aatcctccag gacgacacct
     2701 tgcccctcaa taaagtcgtt gccgtctttg aacagtactt tgaaagcgaa ttcaatgatg
     2761 atgactgggc gtatcccaac atcgtcgtcg acgaaggcca ggacctgagc gactctatcc
     2821 tggaacacct gagcttcctg gcagaattac aggacggctg tttctacatc ttttacgacc
     2881 gcaatcagta catcatgagc cgcaagacga cgggctggat cgataagaat gccgaatgtc
     2941 gcctggtcct ctataagaat tgccgcaata ccgctgaaat cgccacgtcc atcggcaagt
     3001 tgatgcacat gaaagacagt tatgtcaaca ccatccaggg catgacgccg aaagccgtct
     3061 tttatgggaa cgaaaaagaa ctgcagaaac aggcagaccg cttcgtcaag gacatgtgcc
     3121 agcaaggcgt tgccctgaaa gacatggtca tcctcacggc ccactccgtc ggccatagcg
     3181 gcctcaaagg ctgccggacc ctcaacggga ttcccgtcac gacggaaccg gaagacggca
     3241 aaatcctctt cacgtcggta cggaaattca agggcctcga agccacggcg gtcctcctca
     3301 tcgacgtcaa tctgtcggaa ctcccggacg acctcatgca gcgcctcatc tacgtcggct
     3361 cctcgcgggc tacgatgtac ctgaaaatcg ccttctgcga agacatcgaa aagaaaaact
     3421 acggccccat cctggatgcc ctgaccgacg aaccggtcaa gcataaccgc agcggactgg
     3481 tcaaaaccct gcacttagaa gcggactaat gggagcgcag gccagtgaaa ggctctcttt
     3541 ctaggagagc tgtcagcgaa gctgactgag aggttgcttt taaatttaac tccatccgct
     3601 gactgctaaa aaaggcactg tcgtcgtgcg acagcgtctt tttgcctcgc catgacaaaa
     3661 cctgtcctac tcagatgcta tagtgaagat ataaattgta tttcacagga gggatagtca
     3721 tggacagaag cgaacagacc aagaagaatc aggccatcaa tttgtatacg tggggcttag
     3781 aactcctctc tgacggcggc cacgtccggg atgccgataa agtgggctgg atgctgatag
     3841 aaaatgcagc cacccagggt gaccgggacg ctcagaacta tttaggcgat gcctattttt
     3901 acagttacaa aaaagatgaa aaagctgcct tttggtacac caaagccgct gaacagggac
     3961 tgccagaagc ccaggctatg ctggggatgc tctatctcct gggacgcggc gtcacacgga
     4021 attacgccaa agcccgccat tactttgaac aagccttgca gggcggtgaa ctcaacgtcg
     4081 aagccgccgt ggcccaatat tggctgggcc agatgtataa agaaggcatc ggcgtcccga
     4141 aagacagcga aaaaggtgaa aaactgatcc gcctggccgc catcaacggt tatgcacccc
     4201 atgggaaata agtgagcgca ggccgccggt cgcctcggaa tcagatgcgg cttcgccgcg
     4261 acaaactcaa actgacaacg acgcttcggg aagccctcag cgcgtagcgg gccgccctcc
     4321 gctaccatcc gtccgggtaa ccatcctgca cctgaaaaat cagaatggtt tgggacggcc
     4381 cctacggatg cggcttcgcc gcaataaatt catgaaataa acgcagttcg ctggtcgcag
     4441 atggcagtcc gccgcggtct tgcagaccac aaaaaaatca cggccaacgg ccttgtgcgt
     4501 aggggctgtc ccaagccttt ttggcccgtc aggtgccgat cgtcgggagg tgcaggacgg
     4561 ctgccaaaac ggacagctgt ggctgggcgt cccgcttttg agattcgacc atagcctatt
     4621 caagcataat ggcaggcttt tacgagccgc ggctattgtc acaagcggag ggtcactcga
     4681 agcgatgtta tttttctgaa tctgttgtcg ctacgtgcgg taacagattt ataaaataaa
     4741 aacattacgc cggcgtcggc aggcaggtcg caaaaaacac tgcacgaat
//
LOCUS       DAWCYS010000110         4788 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_994_length_4788_cov_4.480668, whole genome shotgun sequence.
ACCESSION   DAWCYS010000110 DAWCYS010000000
VERSION     DAWCYS010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_4788_cov_4.480668"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2702..2711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1310 a         1026 c         1282 g         1160 t
ORIGIN      
        1 agagtggaat gttatgcacg tgtatcgaca gagcatgaag atcaggtgac aagctatgaa
       61 gcaaaggttg attattacac ctcgtatatc aagggcaggc atgactggga gtttgttggt
      121 atatacacgg atgaaggaat ttcggcaacc aatacccggc accgtgacgg gttcaatacg
      181 atggtaaggg atgcgctgga taataagatt gaccttatta ttacgaagtc cgtcagccgg
      241 tttgccagga atactgtgga tagtttgaca acggtacgaa aacttaagga taaggggatt
      301 gaggtttatt tcgagaaaga aaatatctgg acgcttgatg ccaagggcga actgctcatc
      361 accatcatgt cctcgcttgc ccaggaagaa agccggagca tttccgagaa cacgacatgg
      421 ggacagcgga aacggttcgc tgatggcaag gtcagcgtgg gatttagcca tttcctagga
      481 tatgaccgtg ggccggacgg caatttggtt gtcaataggg aacaggcaaa gactgtgaag
      541 ctgatttacc gcctgttcct ggacgggtat accttccatt ccattactaa agaactgatg
      601 gcaagaggac tggaaacccc gacaggaaag aaaaaatggt accaggggac ggtaaaaagt
      661 atcctgacga atgaaaaata caaaggcgat gccttgctgc agaagcgctt caccgtcaac
      721 ttcctgacca aagaaacgaa ggctaatgaa ggggaagtac cgcagtacta tgtagaaaat
      781 aatcacgaag ccatcatcag cccccaggtc tttgattggg tacaggaaga aatcaagcaa
      841 cggcatgaag gcaaaaagcg ttatagcggc gtatccattt tttccagcaa agtcaagtgc
      901 ggccagtgtg gcagctggta cggtgccaag gtctggcact cgacagataa ataccgccga
      961 accatctacc gttgcaacga taagttcaaa caccactgca agacaccgca tctgacggaa
     1021 gaagaaataa aggatatctt cgtgaaagct gtcaatcagc tcatcgaaaa caaagcagat
     1081 gtccttaact gcatcaccct gcggaaggaa cggcttacca atacagaaaa cctggaagag
     1141 gagcgggaca agctcagcac agatttgaac ctgctggccg agaaggtaca gcaactcata
     1201 gccgagaatg cgcgcgtggc acagaaccag gatgattacg atcggaacta cagcgagctg
     1261 gtcagccgat atgaagcggc gaagaagcag tacgacaaga actgtgaagc catccagtat
     1321 cgtgagagaa ggcgccgtca gatagacagc ttcatcgagg agcttcgggg ccaggaactc
     1381 atcaaggagt tcgatgtccg gctttggggc agcatggtgg attttattat ggtatatagt
     1441 aaggaggata ttagggttac cttcaaggac gggacagaaa tcagagcata aagaagatag
     1501 cggtcatcag taggttgaat accatgctga tgaccgtttt ttcgtcttag tttcggttct
     1561 atgatataat aattgtcaag aaagtctgaa agtaggaggt ggaaaaatga atttctatga
     1621 agaagctata gcaagaaagt atatgacttt aattgaggca ttgaaatgtt tgccaggagt
     1681 tatgcccaat aaatcaaaag aattaataga taatgcttta gaaatgaact ttatcccggg
     1741 ttcgttagaa atagcggatt tttgctatcg tgttggtgaa acagaattat tacaagaaga
     1801 atatgaggaa attcatatag aattagaatc tttttatgga acaactataa agacaagccc
     1861 tcaaggggct aaggtactta tgaaattata tcgcaatgga gatttggtta tgcataaagg
     1921 aaaatgcata actgaggcac ctgaattgca ggcatatatt gataaggaaa gttactttag
     1981 ggaagaagct gacagaatta ggcgggaaga tgaatataga aaatacctta tcaagaatcc
     2041 agatgaaatc aaagaatccg atttttcgta ttccctattg aacgatgtgt tctatcacaa
     2101 gtttggtgca tttcggggcc ataaaacaat aattcttgat ggcattaaag ttaataagtc
     2161 cgtattcgtt tatcaaagca attcaggaaa aacacacgat tcagaagtca taattagttg
     2221 gactgattct aagggggaag gtcacaaact atgcaaacct agcttctata gcgagaatag
     2281 aagaaatgat cccgaccgaa attggggcct gccggaatag attgaaggta gtggttttgt
     2341 tttaaaagag aattaagttt gtcatcgaac cataaatata tgcacacggg ggtgttcaaa
     2401 attctgacta tcgttcgatt gtatcaaata cggacacctt tgagacgaca tcttcacgac
     2461 tggtgcgacc ttagaagcct gcgctcatgc cttgaaacaa aaaggcgccg ccagcgtgac
     2521 gggcctcgtc atcgccagcg gtgcaccata aaataaacgc agttcgcagg cggccggcgt
     2581 tcttcaattc gatgaacgat gaacgaccta cgatgaacgt aaaaagcgct gtcttcatgc
     2641 gacagcgctt tttgattaga atgtgtgatt acagtctctt taacacatct gccgatgacg
     2701 annnnnnnnn ncgctttttg aatacaaggt gttattaacc taatgtttca ataaaagctt
     2761 tgaccacttc cggatcaaag agcaggcccg acgcgctgaa gagttcgcgg acggcctgtt
     2821 ttttggtttt cgcccagaat tctgccgacg ggttgatgat catgtcgtag taatcggcga
     2881 cggcgatgat gcgcgccccc aagggaatat tggcgccgcg caggtgtttg ggaaagcccg
     2941 agccgtccca gtgttcgtgg tgatagcgga tgtaaggcac gatgtcctgg cagcagggga
     3001 tgttttccag catgttgccg ccggctgcgg cgtggcgctt gtactgcgcc ttttccgagc
     3061 ggttgaggta ggacatcttg gccagcaggg tattagacac gaagagcaga cccacgtcat
     3121 ggaggagtgc tgcgtatttc acttgttcga tttcactctt gggcagcgtc agtttggcgg
     3181 cgacgctgac gctgtagtta gcgacctgca gggaatgcat atataagcgc tgactcttca
     3241 actgtatgaa attgagcact tccgctgtaa tggcgtcgag ggacgtcatt tcttcccggg
     3301 caatgcccgc agcctgtttt aacgatacca tatacatgac caacaaaccc tttcatctgc
     3361 aaacgtgtta cttgttcgtc gtccgatgtg cccgacgtac cttctgcgga tggcggagga
     3421 tgttgaacat cttcacactc gtcttcttga tgcgtgcacc ggtgtttttc atgcgccgtg
     3481 ccgtcgtgat cttcgatttg cgtttcgggc gcatgtctcg gatagagccg aaatcaccgc
     3541 ggcggatact tgtcgctttg atgcggtctg agcggaggtt taccttcagg gtgctcataa
     3601 tctggctcgt cttgaggtcg atattaaaac tgtagatgtc cactgccacg taggacaggg
     3661 gaggataggc gtgaatacaa atatgtgcgt tgccggaaac ggcaatacac gtcagttcgt
     3721 cgtcgatatg gaagaatgta atcccttcga caggggcgcc gacgaggtcg aacgcgcgat
     3781 ctaccaaggg ctgcaaatct tcggcctccg aaaaggaagc cttgcagtta taaaaatcga
     3841 tgagtacatg ttttcctaat gtgttattca taaatctcca cccgttactt agttacagct
     3901 tattatatac tgttttcggg ctaatgtaaa cagaaaaaaa gacccgttcc cgatgttttt
     3961 ttgcatcagg aacgggtcct ttatgcgcca gccgccagcc actcactgct gacagctgac
     4021 tgctagccgc tgaccgccaa ctgccagggg ttagggccta gcagctaggg gttagtgtct
     4081 gacagcgttt tccacatact aacagctaac agctaacggc taacagctcg ccacactcac
     4141 gcggcatggg cacgcatggg cgtgaaggtg aagccattgc cgtcgtagcc gatggtgact
     4201 gtatcgcctt cacggatagt accggcgatg atgtcgcggc tcagggccgt ttcgacggta
     4261 tgcgtgatca ggcggcgcag cggacgggca ccgaactgcg ggtcgaagcc tttgtcggcc
     4321 aattcctgca gggcttcgtc ggtccaggtg aggtggatct tgacctgttt ttccagacgt
     4381 ttgcccaggc gttccaggag gatagcggcg atattgcgta cctggtcttt ctggagcgct
     4441 ttgaagacga cgatgtcgtc gacacggttg aggaattccg gacggaagta ctgtttaagc
     4501 agttcacgga ctttcttgtt ggcttcgtcg taatccttcg attcgaggat ttcgtgggag
     4561 ccgaggttac tggtcatgat gatgatggtg ttcttgaagt tgactacacg gcctttgcca
     4621 tcggtcaggc ggccatcatc gagaatctgc aagaggacgt tgaagatgtc cggatgggcc
     4681 ttttcgattt cgtcgaggag gatgacgctg tacggacgac ggcggacggc ttcagtgagc
     4741 tggccccctt catcatagcc gacatatccc ggaggagccc cgatcagg
//
LOCUS       DAWCYS010000111         4770 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1001_length_4770_cov_10.539343, whole genome shotgun sequence.
ACCESSION   DAWCYS010000111 DAWCYS010000000
VERSION     DAWCYS010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1001_length_4770_cov_10.539343"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1120 a         1337 c         1194 g         1119 t
ORIGIN      
        1 caaatttcga tatctctgta ttaggatggg cacgaaacat aatatgtaca tggtcctggt
       61 cgtggttcca ttcttccaaa gtgatattat atgatgaacc tatgcggacg aatatatctt
      121 ttgcatattg gctcattggg tcagaaaata ctttgcgtcg gtacttcacc actaaaacaa
      181 gatggtaata gagtaagaac actgaatggt tattactgtc taattccata gtataatcag
      241 tcctttctct cttactactg attatactgc aaaacaaatt atgaagaaat tgtctttctg
      301 agggcatcga accctcagaa aaacctgcaa ttcatctcac cgcctaaaga ggcaggaaac
      361 ttcttgcaga atcaggttaa actttattat accatattta tgtccctgcg agaatcagca
      421 ggcaggacag aatcgccgca atctgtctta tttacatggt ctatatcttg ggataaaata
      481 atagtacagt ttacttttag gaggtttttt ctatggcaac taaacgcgtc ctcgcaatcc
      541 acgatatgtg ttctttcggc cgctgctccc tgacagccgc catcccggtc atttcggcca
      601 tgggcttcca ggtctgcccc ttcccgacgg ctattttcag caacaacctg acgtatggcg
      661 actatacctt ctctgatttt acgccgcaca tgacggaatt catggataaa tggcagaaac
      721 tgggctattc gtatgacgcc atttacagcg gcttcctcgc cgacgccggc cagattgcca
      781 tcgtcctcga cgccatcgcc cgcttcgcca agaaggatac cctcgtcgtc gtcgatccgg
      841 ccatggccga tgacggcaag ctctacccgg tcttcaagcc cgatattgtc atggaaatgc
      901 gcaagcttat cgccaaagcg accatcatta caccgaatta taccgaagct gccctgctgg
      961 ccgacatccc ctatgcccag ggcgcgccat ccatgaagac tatgctcgaa ctctgccaga
     1021 agctggccgc catgggaccg cgtcaaatca tcatcaccag tgtccccacg ggcaacgaaa
     1081 cgatcaccgt cgtcagctac gacgcccaga cgacgtcttt cgacgaatgt accgtcgacc
     1141 gcatcccctt cagcacctgc ggcacaggcg acctcttcac cagcgtcctc acaggttccc
     1201 tgctccaggg ccagtccctg cacgacagcg tcaaaaaggc cacacagttc ctgaactacg
     1261 ccatcggcta cacctaccag tccggcagcg atccccgcga aggcgttcag gtcgaaccgt
     1321 gcctgaaaaa gctgttctaa gttttattgt taacttatat aaatccatat aaagttgacc
     1381 tcatgagcgc ttttttgcta taatacggaa gaagtccgta agcaaaggag cgcttttatc
     1441 atgtctgatt caagagaaaa gacgatgcgc ctgacgcgca tcgccttatt gattgctatg
     1501 aactgtattt ctgcctattt gatcattccc ctgccctttt cccagtcgcc catcgccttg
     1561 cagacgctcg tcgtcaacct ggtcgccttc ctgctgccgc cgaaagatgc agccatcacc
     1621 atgctggcct acatcggcat cggcatcatc ggcgtccccg tcttcacagg cggtacggca
     1681 ggtcccggca agatgttcgg cccgacaggc ggctatatct gggcctatgt cgccgccgtc
     1741 ttcctcatgt cctggttcaa agggaaggaa tacaacttcc gtcgctactc tctggcagcc
     1801 gtcgccatcg gtatccccgt tatctatatc ggcggcgtac tgcagctcaa aggcatcacg
     1861 ggaatgccct gggaagcagc catcatgagc ggcgtcgtcc ccttcatccc cttggacatc
     1921 gtcaagagcc tctgcgctgc cgctctggca cggcccttat tggccataaa taaaaccgta
     1981 tctcacgaat gacgccagct tgtgtaaatc cgttctttgt cagtatacat ctcccgatct
     2041 gcctgggata acacctcgtc gatattggca ataggcgtct ggcagacgac aacgcctaag
     2101 gccatgctga tgccgctgga acggtaacgg gccagcagct cgcggacgag ctgctgagcc
     2161 tgctgttcac tggcattcct ggcgatgacc atgaattcat cgccgcccat gcggaatacg
     2221 gcgttatcgc cgctcaaggc tttcatatgc cggacagcct gctggaggag atggtctccg
     2281 gcttcatggc cttgcgtatc attgattcgc ttgaggccgt tgaggtcgcc aaaaataaag
     2341 gccaggaaaa tcccatcggg cacagcccgg atatattcgg taaacccccg gcgattgccg
     2401 gcgccggtca gctggtccgt cgtgctgagg cgttccagcg aacggaaagt atcgcggttg
     2461 cgcagcatac tggccaggaa caaggtcagc gtcgacaaca ggaggctggc cgatttaaag
     2521 gccaatgccg acgggttgac gacttccgta aagcccaggg acttgcccga ttgagtgaga
     2581 tgtcccgata cgaggcggcg gacgccggaa agatgcggcg taaatccagg atgagcccgg
     2641 acgaaagcct gtgcatcttc aataatggcc atctgtttag catcaaaggc cgcatacagc
     2701 ggtcgcaggg agtccatggg aatgtgctgg accgtaggag ccatcggctg caaatcctct
     2761 tgataccatt cataggtagc gctgacggtc tggtccggct gttcctcaaa aatcaggacc
     2821 cgttcggcct gcaggctgcg gccgatatgg gccagcatct tctgcaagcc cacgctggga
     2881 tcggcttcgc gcataccgat ggcgatgacg tcgttagcca tggcttcgcg gaaaatgacg
     2941 cgattttctg ctaagtcccg gttgatatac tgattgatat cggctgccat agaaaagcgg
     3001 taattcttcc cttgccaggg aatgagcgtc gtccgcatga acaagttctg tccagtcttg
     3061 cggttgcggc gcgtcaccgt acagaaacaa tcgcggcgca gctgaccgtt gatgcagtca
     3121 tcgcaaggcc tatcgaggcc gtacaggagc ttataacact tttccccaac ccagctgtaa
     3181 gcgtcggaaa agccatagct cttctgcata tactcgttga catagaccag ttcatacgta
     3241 tccgggtcga tgatggacac ttcttcatca agcttatcga atttttcctt gtcgaagacc
     3301 aggcggtcgc cgatgtagac ggcctggccg tagcgctgtt cttcggcatc tttcaggcgc
     3361 tccctcagga gttgcatgat ttcatcgaaa tcccggcctt ctgagccctg gacgatggca
     3421 tactgcaaat agagggtgca gctgtagccg ccgatatcgg taaggctgtg gatggcgttg
     3481 gtcaggccca gcagggtatt gcggaagtcc cgatccaggc tctgatgggt gatatagaaa
     3541 aagcggcagc cgctcagatg ggccagggag cctttcaaag agtggagcgt caaaagctgg
     3601 tccgtaatgg cctgcaataa ctttccggcc acttcgtcgc cgtaatcccg gaagacttgt
     3661 tcatattccg gcacttcgag tacgacacag acataatccg tcccgtaccg ccgataagct
     3721 tccatgtaat cttcgccgac cagcaggagg ccccggaaat tgagcagccc cgtttcggca
     3781 tcaatgaggc tgaccgtatg gagctgtgcc tggtgccggc tgctttcgtc gacatcctcg
     3841 aaatacccca gcagaccgat gatacgcttg ccccggtaaa tggggaattt cgtagaggca
     3901 atctggcgca gccgtccctg gaccaggcat tgacccagaa catcgcgcgt gacttggccc
     3961 ttttccagga cggccatctc cgcttctctg tacacacagt cgttgacaat ccagcccaag
     4021 tcctcatcgg ttttgccgat gagggctttt tcgtccttcc ggccatagta gtcgaggaaa
     4081 gactgactga caccggcaaa gcggcgggcc ctgtctttcc agaaaaactt gatgcccgaa
     4141 tagtgaatca tagactggca gagttcccgc aagaggcgga aataaggctg ccgggccggt
     4201 tccagctcgt ggatgccaaa agaggtggcc agtatgggca gcaggctctt gcggtcccgt
     4261 gccgtttccc agacgtcttc gcggcagcac agcaggagat cctgccggtt gtcgacgcgg
     4321 aggaggagga ctaaaaagac ggtccaatgg taactgccat cagctgtccg aaagcggaag
     4381 gtgatgaact tcggacgccc tgcatcatcg gctttatcca ggggccggta atcagcggct
     4441 tcgatcatac ccttgcggac tttccccgtg aggcgataga actccccttc atcaccgcaa
     4501 tggtacatat tcaacagccc gcggttaacg aagagaatcg gttctgattc atccataccg
     4561 atcaaacaga cgccggcaat cagtttatca taatagcgat gcaattccgc ttgtatcgat
     4621 gccacatcca taaagtccac ccttttctgc aaaaacgttc cgcaataaaa aatagagtca
     4681 tcgtatataa tctattataa cacatgataa aaagaggcta tcgcattaag acagcctctt
     4741 tttgagtttt aagtttgtag tttttagttt
//
LOCUS       DAWCYS010000112         4724 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1015_length_4724_cov_4.450203, whole genome shotgun sequence.
ACCESSION   DAWCYS010000112 DAWCYS010000000
VERSION     DAWCYS010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1015_length_4724_cov_4.450203"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1376 a         1267 c         1201 g          880 t
ORIGIN      
        1 tcgccaagct ctatgaagcg tcggcagccg gcgtcaagat ccgcctcatc gtccgcggca
       61 tctgcgtcat ccgccctggc ctgcctggca tcagcgaaaa catcgaagtc cacagcatcg
      121 tcggccgctg cctggaacac agccgcctgt tctacttcta caacggcggc aaggaagacg
      181 tcttcatttc ttcggcagac tggatgccgc gcaacctcaa cgaacgcgtc gaattgatga
      241 cacctgtcga attcccgccg cataaagaac ggatcaagcg catcctgaaa gtatacttcg
      301 acgacaacgt caaggcctat gctatgaaca gcgacggctc gtaccgctac ctggcagacg
      361 aacgcaaggg caagtctgtc aacgcccagg atacatgcca gcgcgaagct gaacggctgg
      421 catcggaacg gaagaaggca gcccgccagt tccgcaacgt cgtcctgcgc ccgcgtcctg
      481 ctgaagaaga caagtaaatt gacaagcctg ccggtctttg ctacaatgaa aagaaaagca
      541 ttaggaggca tggcatggac tggaaacgat tcagcatgac tggattcaag aataaacgac
      601 cgattacacg catgtacgcg acgatgatcg ccttattggt catcgtgtcc ctgacagggt
      661 tcatcgacag ggggaaatca gtgaccctcg tcgccgatgg cgaaacccgc caggtatata
      721 cccacgctgt caatgaaaag gcattgatgc gggaaaacaa tatcgacatc ggtgattacg
      781 atgaaatcga tgtttcgacg cagaaactcg acgacggcag ctacgtcaag gtgcgccggg
      841 ccatcccggt caccctggag taccgcggac agacccagga agtctacacg gccaagcaca
      901 attcccagga agtcgccgaa cagtacggct ataataagga aaagtaccgc ccttatggcg
      961 acgtccagaa accgctccag gcggggcagc acatcgtcat cggccacgta tcgcgcaaga
     1021 ccatcacgga agatgaagtc gtgccctatg ccatcgaaag catccctgat gaaaccttgg
     1081 gccagggcga agagaaagtc atccaggaag ggcagaacgg ccgcaagcgg gtcaagctga
     1141 acatcgtcag cgtcgacggc caggtcgccg acaaggaaac gctgtcgacg gagctcattt
     1201 cggaaatgca gcctctggtc cgcaaagtcg gcactaagga aaccgttgaa atcaatacca
     1261 gcacaggtga tgtatcccac taccagaaga tcatcaccat ggaagcgacg gcctatctgc
     1321 cgacggacgg cgacggccag tgcatcaccc gcatggggac gcgggcccgt tatggcgtcg
     1381 tcgctgtcga ccccaacgtc attcccctgg gctcgacggt cttcattccc ggctatggcg
     1441 tagcccgggc cgaagataca ggcggagcca tcctcggcaa ccgcatcgac ctctgcatgg
     1501 aagactacga tgcctgcatg aacttcggcc gccacaccgt accggtctat gtcctgaatt
     1561 aaatttttca ttatccgcta cgaactgcaa acttacaact caaagcaagg gcaggcattt
     1621 gaaaaaatgc ttgcccttgt ttgtcatata tgctataata ccaaaggttt gaataccaaa
     1681 ccctaaaata cacacgcttg gcgagctcag cactgtgtcc gttccccgtg gggaagggtg
     1741 aagatgagcg atgaccgggc ggaggaaaaa acaggaggaa ttacaattat ggcagtagta
     1801 tctatgaaac aattattgga agccggcgtt catttcggtc atcagacccg ccgttggaac
     1861 ccgaagatgg ctcgtttcat cttcaccgaa cgtaacggta tctacatcat cgacttgcag
     1921 aaaaccgtta agaaaatgga cgaagcttac aattttgtcc gcgacctggc tgctgaaggc
     1981 ggcaaagtcc tcttcgtagg cacgaaaaaa caggcccagg aatccattaa aaacgaagca
     2041 gaacgctgca accagtactt cgtcaacgaa cgctggctcg gcggtatgct caccaacttc
     2101 cagaccatcg aaaagcgtgt aaaacgcttg aagacattgg aaaaacaggc cgaagatggc
     2161 acgttcgaac tcttgccgaa gaaagaagtt acgctcctca aacatgaaat ggaaaaactt
     2221 cagaaatacc tcgccggcat caaagacatg aagaaactcc cggacgctct cttcatcatc
     2281 gacccgaaaa aagaagaaat cgctgtttct gaagcccgca aacttcacat ccccatcatc
     2341 gctacggttg acaccaactg cgaccccgat gtcatcgact atccaatccc ggctaacgat
     2401 gacgctatcc gcgccgtcaa actcttgacc ggcaagatcg ctgacgctgt cctcgaaggc
     2461 aaccagggcg aacagactga agaaggcgcc gctgaaggtg ctgaagccgc tgaagaaaaa
     2521 gcagcagaat aagattactc gttctttgag aactgaatgg ttattataga aaggtaagga
     2581 gcctttgctt cttacctttc ttcaaactgt ataattcaca ttacttttag gaggaatact
     2641 catggctatt acagcagcaa tggtaaaaga attaagaaca aaaaccggtg caggcatgat
     2701 ggactgcaaa aaagcattga ccgaaagcaa cggcgacatg gaaaaagccg tcgacttgct
     2761 tcgcgaacac ggcttggctg cagcagctaa gaaagcagac cgcatcgctg ctgaaggttt
     2821 ggtttattct tacatccatg gcaacggccg tatcggcgtt ctcgtcgaag tcaactgcga
     2881 aacggacttc gtcgctcaga cagataactt caaagcactc tgcaaagaca ttgcaatgca
     2941 gatcgctgct gcaaaaccgg catacctcaa acgcgaagaa gttccggctg acgtattgga
     3001 acacgaaaaa gaagttctcc gccagcaggc cctgaacgaa ggcaaaccgg aaaagatcgt
     3061 cgaaaagatg attgtcggcc gtatcgaaaa attctacaaa gaaaactgcc tcctcgatca
     3121 ggaattcatc aaagattctg acaagacggt ccagcaggtc gttacggaag ccatcgctaa
     3181 gatcggtgaa aaaatcgaca tccgccgtta tacgcgttat gaattgggtg aaggcctgga
     3241 aaaacggaac gatgattttg tatccgaagt aatggcacaa gctaaataag attgcataag
     3301 agaacaccca acccgtgttc tcttattttc taattggact tgtataccga cagaggaggc
     3361 acttctttta tggaatcgaa aaagtatcat cgcatcgtat tgaaattgag cggcgaagca
     3421 ctggctggca aacagggtta cggcatcgac ccggaaacgg tcgaaaccat tgccaagcag
     3481 gtcgtcgaag tcaataagct cggcgtccag gtcgccatcg tcgttggcgg cggcaacatc
     3541 tggcgcggcc tgtccggctc ggctaaaggc atggaccgcg tctcggcaga ctacatgggc
     3601 atgctggcta cggtcatgaa ttccctggcc ctccaggacg ctatggaaaa gaacggcgtc
     3661 gctacccgcg tccagacggc tatcaacatg cagcaggtcg ctgaaccgta tatccgccgc
     3721 cgggctatcc gccatatgga aaaaggccgc gtcgtcatct tcggtgccgg tacgggcaac
     3781 ccttacttct cgacggatac gacagccgca ctgcgggctg ctgaaatcga agccgatgcc
     3841 atcctcatgg ccaagaacgg cgtcgacggc gtctatgaca gcgatcccaa actcaatccg
     3901 gatgcgaaga tgttcaagca tctgacctat ctcgacgtca tcaacaaaga cctgaaggtc
     3961 atggatgcta cggctacgac gctctgccgc aacaacaaca tccccatcgt cgtcttcaac
     4021 atcgaccagc cggggaacat cgtcaaagcc gccagtggag aagatttagg cactgtagtc
     4081 gacaaagaag actaaaagct gtatgatata cttataaaaa taaaataagg aagtgattga
     4141 catggaagta aaagaaatca ttgcccagca cgaagaaaag atggacaaga ccatcgaagc
     4201 cctgaaacgg gaattgtcgt cgatccgcac gggccgcgcc agcacgtcgc tgctggaccg
     4261 gatcaccgtc gactactatg gtacgccgac gccggtcaaa caggtcgcca atgtggccgc
     4321 tccggaaccg cgcatgatca ccatcgcccc gtgggaaccg aagatgctcg gtgaaatcga
     4381 aaaggccatc ctcaagtccg acttgggcct gaacccgaac aacgacggca agatcatccg
     4441 cctggaactc ccccagctga cggaagaacg ccgtaaggaa ctgagcaaga aagtcagcaa
     4501 gattgccgaa gactcgaaag tcgtcatccg caatatccgc cgcgacgcca acgaaaccat
     4561 caagaagatg gaaaagaaga aggaagtcac ggaagatgac agcaaagaag gtcaggaaaa
     4621 catccagacg ctgacggata aaaaaatcaa ggtcatcgac gaactcaaag cgaaaaatga
     4681 aaaagaagtc agggccgtat gatggatagt cggagtttta ttgg
//
LOCUS       DAWCYS010000113         4724 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1014_length_4724_cov_6.097237, whole genome shotgun sequence.
ACCESSION   DAWCYS010000113 DAWCYS010000000
VERSION     DAWCYS010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_4724_cov_6.097237"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1020 a         1186 c         1367 g         1151 t
ORIGIN      
        1 gccaggggca gtaaggcttc gtacagggaa gcgccgccga tgccgaaata gtcctccccg
       61 gcttccatgg cctgctgcat ggcttcgaca ctgtggaaga tagtgacggc gtcatggctg
      121 acgctgtaat ccttttgatg ggtcaggacc cagtgcggcc ggccgggcag gacgccgggc
      181 aggctttcga aagtcttgcg ccccatgagc atcgtatggc ccatggtgag ggatttaaaa
      241 cgttttaagt cggccgaaat atggcacaag aggtcgttgt ccttgccaat accgccgttc
      301 cggtcgacgg ctgcaatgag atggatcatc agacggccac ctccatgggc agtttaccca
      361 agtgccggta attgtccagg gcgatgtcgt cgatggtgaa atcatagaag tcgtggacat
      421 cgggattgag gaccagcttc ggtgccggaa aggcctggtc acggcgggcc agctgggtct
      481 tcatggcctg gacgtggttc tcatagacgt gggcgttgtt gatgacgtgc gtgaacaggc
      541 ccggacgcag gccggcggac tgggcgatga gatggatgag gacggcatac tgggtcatgt
      601 tgaaggggac gcccaggccc atgtcgccgc tgcgctggat gagcatgcag ttcaggcggc
      661 cgtcagtgac atcccacaag gtctggtagg cacagggctg aagggccatg tccggcaggt
      721 cttcgatgtt ccacagggat acgatcatgc gccggttctg aggatccgtc ttcagcgttt
      781 ccaggagctt gtcgacctgt ttgtacttgc ggatctgata gccataggcc ttgccaatgg
      841 tgccgtcggg ccgcatccat tcgtcccaga cgtggcagtt catcttctgc agttcccgca
      901 cgtcgttgct ctggagctgc cagatccaga ggatttcttt gacagctgtc ttgaaggcga
      961 cgaacttcgt cgtcaagatg gggaattctt tttccaaatc gaactgcatg atctggtggg
     1021 gcagtttata ggtccgcacg cccgtgcggt tgttgtcgaa atagccgtgg tcgaggatat
     1081 tttcgacgat attcagatac tgtgtatcgg caatgctcat gtatgtcacc tcaaaatcaa
     1141 tggttatcta tataagactg gatggcgctg acgaactggc ggccatattt atggagcttg
     1201 aagctgccta cgcccttgat gtcgctcatg gcttccagac tgtccggctt gagagcggcc
     1261 atatcccaca acgtggcatc ggaaaagatg acgaacggcg gaatatgctc gtccttggcc
     1321 agttccaggc ggactgcccg cagggtatcg aacaggggcc gcaaggcgtc ttcgtcgatg
     1381 gccccggcgc ggcgcttctg ctggcgcttg ggcagttccg gcacgggttc agctgaaacg
     1441 actttctgct ggacaatgcg ctgatggccg ctgagggcct gccgtccgtc ggccgtcagg
     1501 gagacgacgg gatatttccc atcggactgg tcgagatagc cgtcgtcgat gcactggcgg
     1561 accatgtcgc ggacttcgga caaagtgaaa tcgccgagca tgccgaaggc cgaattatgt
     1621 tcaaagccat agcggcggac cttggcgttc tggcttccct tgaggatgtc gcagaccatg
     1681 gtcatgccga agcgcccttt caattcgtcg acgcagaggc agatgctgcg gacctgttcg
     1741 gtcatgtctt cttcgaccgt ttcctggtcg cagttgccgc atttctcgca gcgctgccac
     1801 gatggatgtt cgccgaaata ggtcaggata taatggcgca ggcagtgatt gccttcgcag
     1861 taacggacca tggcgttgag caggcggaat tcgacggcct gctgctgcgg atcgtggacg
     1921 gactgttcga tgaggaactt ctggatcatg atgtcctggc ggctgaagag gaggatacat
     1981 tcccccggcg caccatcgcg gccggcccgg cccgcttcct ggtagtagct ttcaatgttc
     2041 ttgggcatct ggtaatggat gacgtagcgg acgttgctct tgtcgatgcc catgccgaaa
     2101 gcgttggtag cgacgatgac ctggagccgg tcatacgtaa aggcgtcctg catggtccgg
     2161 cgcatgtcgt cgctgaggcc ggcatgatag cggccggccc ggatgccccg ccgcgtcagc
     2221 tcttcataga cgcggtccac ttccttgcgc gtcgccgcat agatgatgcc gctgtcttcc
     2281 ctatgctgct ggacgtagga caaggcgaag tcgagcttgc ggttcatccg gacgacgcgg
     2341 aaatacagat tcggccggtc gaagccgccg atgaagatgc gttccttttc caggcccagg
     2401 aggttcatca tgtcctcttt gaccttttcc gtcgccgtcg ccgtgaaggc cccgacgaca
     2461 ggccgctcgg gcagggccgc aatccagtcc ttgatggcgc agtagctggg ccggaaatca
     2521 tggccccact gggatacgca gtgtgcttcg tcgatgatga acatggaaat gggcaaactc
     2581 tgcatgaagg acgtaaagaa ttcgttttcc aggcgttccg gcgaaatata gaccagtttg
     2641 acccggccgg cgcggatggc ggcgaagcgg gccttggcct cttcaaaagt acactggctg
     2701 ttgatatacg tcgccggtat ctcctggttg accaggctgt cgacctggtc cttcatgagg
     2761 gaaatgagcg gcgaaatgac caacgtcacg ccgggcatca tcagggccgg cagctggaag
     2821 catatcgact tccctgcccc tgtcggcatg atggccaggc agtcgcggcc atcgagaagg
     2881 gtctggatga cctcatgctg gcccggccgg aacgacgtat agccaaaata ttgtttcaaa
     2941 agagattcga gttccatcaa gataatcaac ccatctacga aagactaaca actcagcttt
     3001 actgctgata aatttgagtt tatagaatag agtttgtagt ttgtagcagt tggttttaaa
     3061 ttttttagcc attcactaaa aactacaaac tacaaactac aaacttaatg ctcaaaaagg
     3121 agctgtcgta agcgacagct cctttatatc atatcatctt tagccttatt ttgccaattc
     3181 ttctttcaag acgtcggcca ggcgtttgat accttcgacg atgcggtctt ccggcatgtt
     3241 ggagtagttc aggcggaagt ggttggcatt gccgccgtgg gggtagaagg gaccgcccgg
     3301 cacgtaggca acgttcttag ccaggacttt cggcatgagt tctttcgcgt ccatgccttc
     3361 cggcagcgtt acccacgtga acaggccgcc ttcggggtat gtaaaggtga tgccttcagg
     3421 gaagtatttc ttcatcgatt cgcacatcag gtcgcggcgt ttcttataga gggccttgat
     3481 cttggcgacg tgttcatcga ggtcgaacat atcgatgtag tacgaggtct gacgctggcc
     3541 gaaggacgac gactggaggt cgacggcctg tttcagcttg acgactttat cgatgatgac
     3601 cgggtcggct accatccagg caatacgaag gcccggcata aggattttcg agaaagaacc
     3661 gagaaaaatg atatttcctt tcgtatccat cgatttcagg gacggtacct tgtcgccgtc
     3721 atagcgcagt tcgccgtagg ggtcgtcttc gaggaccggg aagtcatagc ggttgatgat
     3781 gtccatgaaa gccttgcgcc gttctacggg ccaagtgatg cccgtcgggt tctggaattc
     3841 cgggatgacg tacatcatgc ggacgcggtc gccgtatttt tccagaatct tttccaattc
     3901 ttccgggatg atgcccttat cgtccgtcgg cacttcgacg aattccggtt tacagagttt
     3961 gaatgcgttg atagcgccga ggtaggtcgg gctttcgacg aggatgacgt cgcccgggtt
     4021 aatgaagatc tgggccagca tggacaggcc ctgctgggac ccagacgtaa tagtgatgtt
     4081 atcgatatcg cagtcgacca tgaaagcctg tttcatgcgg gctgcaatct gtttgcgcag
     4141 ggggttgtaa ccttctgtcg tgccgtactg caaggcttcg cggccttctt tggtcaatac
     4201 ggcgtcgtcg actttcttca gctgttccgt cgggaagagt tccggtgccg gcaggccgcc
     4261 agcaaaggaa atgacttccg gcatggtcgt cagtttcaag agttcacgga tttcagaggc
     4321 tttcaaattc atagctaagt cggaaaaatg catatcaatc actccttcag gttatgtaca
     4381 tcacgcgttt atctatgtgt atatagtatc atcaaaatta cttttatgca attaaacgtt
     4441 tgcgttataa atatgttgcg tttcgtacaa cctttataaa ccaaatgtaa ataacgagaa
     4501 ttactgataa tagtaaatat ccagcttaaa aaaaattaaa gaatgactgt aaaaaatttt
     4561 taaaaaaatt tgcaaaagaa aagacgccct cacgagagag cgccttgttt ccttaaaatt
     4621 taattattta ccgatttttt ctttcaaaac ttcagccaaa cgtttaatgc cttcgacgat
     4681 gcggtcttcc ggcatgttgg agtagttcag gcggaagtgg ttgg
//
LOCUS       DAWCYS010000114         4675 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1023_length_4675_cov_7.010606, whole genome shotgun sequence.
ACCESSION   DAWCYS010000114 DAWCYS010000000
VERSION     DAWCYS010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_4675_cov_7.010606"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1160 a         1360 c         1284 g          871 t
ORIGIN      
        1 cgtcaagaag ggtcagttcc tggccacgga agacatgaag aatgtcgtcg ccaagatcga
       61 acacgcttgc aatgaaaata tcatgctcac cgaacggggc atcagtctgg gctatcacaa
      121 cctcgtcgtc gacatgcgtt ccttcccgat tatgcgcagc ttcggctatc ccgtcgtctt
      181 tgatgccacc cacagcgtcc agctgccggg cggtgccggt acgaaatcgg caggccagcg
      241 ccagttcgtc ggcaacctgg cacgagccgc tgccggtgcc ggtatcgacg gcgtcttcat
      301 ggaagtacac gataacccgg cagaagccct cagcgacggg ccgaatatgc tctacttatc
      361 gcaggtcgaa gatgtcctcc gcgatatgat ggctatcaac gaaatcgcac agaaaagcgt
      421 aagcacggac aggtaaggag acgagaccat gatggatatt ttagaagaag caaaagacgt
      481 gctccatcag gaagcacaag gcattgaaaa gttgatccct acgctcaacc agaacttcgt
      541 caatgctgtc aacatgatta tgaaatcgaa aggccgggtc atcgtcacgg gcatggggaa
      601 atccggccac attgcccgca aagtatcggc aaccttgtcc agtacgggga cgccgtccat
      661 tttcctccat ccggcagaag ccatccacgg cgatttgggc atggtcacgg cagacgatgt
      721 cgtcctggcc ttctcgaaca gtggggaaac ggtggaaata ctcaatatcc tgccgtcgct
      781 caagcgcatc ggtgccaaac tcatcaccgt cgtcggcaat cacaattcga ccttggccaa
      841 acatgccgat atcatcctcg acgcatcggt cgaaaaagaa gcctgccccc tgggcctggc
      901 gccgacgacg agtacgacgg cggctctggc cttaggcgat gccctggctg tcgtcctctt
      961 gacgcagcac catttcacca agaaacagtt cggcatcttc catcccggcg gagccctggg
     1021 caagaaactc ctgctcactg tcgacgatgt catgcataaa ggccaggaca acccggtcgt
     1081 cagcgaagac agcatcgtcc aggatgccct gttcctcatg accgaaaagg gcctgggtgc
     1141 cgtatccgtt gtcaatgaag acggcaaact cgtcggcctg gtcaccgacg gcgacgtccg
     1201 ccgcggcctg gaaacagggt ccaacttcct ccagtggcct gtcgacgcca tgatgaccaa
     1261 gaacccgcgg gtcattgcca accataagct ggctacggaa gccctgcaca tcatggaaaa
     1321 gaaccagccc cgtccgatta cggtcctgcc tgtcgtcgac aaggatgaca aagtcgtcgg
     1381 catgatccat attaccgatt tgctgcggca gggagtggtc tgatgagctt tttgacacat
     1441 atcaagatgc tggccctcga cgtcgacggc gtcatgacgg acggcatcat ttactacagc
     1501 ccgtcgggcg atgccatgaa aggcttttct gcccgcgacg gcatgggcat ttccctgatc
     1561 cgggccgccg gcatccagac ggccctggtc acaggccgcc gctcacccat ggtcgagcag
     1621 cgggcgaagg atctgcatat cgactacgtc ctccaggact gctcgcgcaa gcttttggcc
     1681 atgcagcagt tgtgcagtga aatcggcctg tccctcgacg aagtggccta catgggtgac
     1741 gacctgaacg acgtcgaact cattgccaat gccggtttcg gcgcagcgcc cctcgatgcc
     1801 tgcgaagaag cccgtcggtc ggccgctttc gtatcggcct atcccggcgg ccacggggcc
     1861 ttgcgggaat tcgccgaata tatcttgaag agccagcatg tctgggataa ggtcctgcgc
     1921 cagtactttg gcgacgcgac ctttacgttg cgccaataag aaaggagagt ctgtattgtg
     1981 agtaaggaat ggcagtatca tgctgcacgc ggattcggca agattctctg tcatctgccc
     2041 tatccggtta tcatttcctt gggtcgcaaa ctaggccctt tcgtaggaaa actgatgaag
     2101 aagcagcgcg aacgcggtat tttccagata cagcgcggca tggagtgcag ccgtgaagaa
     2161 gcggaacgca tcatgaccga cgtcttccgc aatctcggcc agtcgctcat ggaaatcctc
     2221 tatacgccca atctcaatcc ggagaatatc cgggactatg tgacattgga ccatccggaa
     2281 cgcctggacg aagccatgaa ggaaggcaaa ggcgtcatcg tcctcacagc ccacatgggc
     2341 aactgggaat ggctcggcgc cctggccatc tacggctatc cggcgacgac gatcgtcaag
     2401 aaccaggcca acgacgccgt aacgcgcctg ctcaacgaaa accgtgagaa catgggactg
     2461 gaagtcttcg cccgcggcgg caatgaaatg atcatcgctg cccgtgcctt gaagcgcaag
     2521 acactcctgg gtttcctggc cgaccaggac ggcggcttcc acggcgtgcc tcagcctttt
     2581 ttgggcaaga tgagctcgac gccgaaagga ccggctatgt tcgcccagaa gttccattca
     2641 cctatcgtgc cgattttccc ggtccacgac gaaaatcatc atacccattt gatgatcggt
     2701 gaaatcatgc atttcgaaga cacaggcaac aaggacgaag acattgcccg catgacccgc
     2761 aagatggccg tcgtcacgga gaatttcatc aaggaacacc cgacagaatg gctgtggttc
     2821 cagcatcgct ggagtacaga accggaagaa atcatagcac tgcagcagaa accggaggcg
     2881 cataccgatg atagccgaaa agctgaaaaa gaataaaatc tccatcattg ccgtcatcgc
     2941 cgtcatcggc tttgccgtca tgctctatag tttcatgcat ccccagaaga cggaagaccc
     3001 catggtcagc ccggataagc tcgtcgaatt cgaagggacg gagctggaag aaaagaaaga
     3061 cggccagctc gtctggcgct tgacagccga taagatccag atcgacccgg acacgcagat
     3121 catgtatttc accaacccca aggccctggt cgtcgccgaa gatggaacgc agatgaccat
     3181 tacgtcggac aagggcatcg tcgaccgcca gaagcggacc atcgaaatca agccgcctgt
     3241 caaagcagaa accgataagg gtgatacgtt gcagaccgac ggcagtgtgt attataatat
     3301 ggatacgcgt atgatcaaag gcggcaaggt cgtcatggac cgccatgaca agacgtcgct
     3361 caaagccgat gcttttgaaa cggacagcag cctgaccaag gtgacgctga ccggccatgc
     3421 gcaggtgaca aagggagaat aactatgatg gatttacttg gaaagaaact ggcagctgcc
     3481 gccctcatgg gggccatgat cctgccggct gccggcgccc tggcagcgga taatgacggc
     3541 aacccggccg tatccgtcac ggcagacacg ctggaatata acggtaagac ccaggtcgct
     3601 acggcgacgg gcaacgtcgt catcgtccgc gaccaggcta cgatgaccgg caacaaggcc
     3661 gtctataact tgaagaccgc cgaagcagac atggaaggca acgtatcggt ccagcagccg
     3721 gacatgcagc tcaacgccga taaggtccat tcgaccaaca agaattacgt cgtcgctaca
     3781 ggcagcgtcc gcggcgtcta cggcgacaag aaggtcaatg gcgacaaagt cgaatactat
     3841 ctcgaccagg attacggcat cgtcacgggc aacggctatc ttgaagccca gggttcgcag
     3901 atgtgggccg atcacatcga cgcctggttc aagcagataa aggccatcgg tacgggcaac
     3961 gtccatatcg aatcgccggc ggaaaacctc atcgcctatg ccaatcaggc cgtctacacc
     4021 cagacgccgg ggcagaatga cggcgtcatc cacttgacgg ggaatgtcaa tgccactcag
     4081 aacggcaatt ctctcaacgg cgacaatgtc gatatccgcc tggccgataa ttccgtccag
     4141 acccgcagcc gatcgactat cgtcatcgta ccgggcagta atccgtaatc gtaaggggta
     4201 tctatgttta ttcagactga agatctcgta aaagcgtata aagaacgaaa tgccgtcaac
     4261 ggcgtcagta tccgcgtcga ccagggccag atcgtcggtc tcctgggacc gaatggcgcc
     4321 ggcaagacga cgactttcta catgatcgtc ggcatcgaac ggccgacgtc agggaagata
     4381 accattgatg gcaaggacat caccaacctg cccattcaca agcgggcggc ctatggcatc
     4441 ggctatctgc ctcaggaagc gtcgattttc cgcaagctca ccgtcgaaga caacctcctg
     4501 gccatgctgg agacgacgaa gctcagcaag aaggagcagc atgaaaaagt ggaagccctg
     4561 atggaagaat tttccatcac ccagctccgc gaccgcctgg gtatccagct gtccggcggc
     4621 gaacggcgcc gcgtggaaat cgcccgctgt ctggtcatgg acccggcatt tatcc
//
LOCUS       DAWCYS010000115         4644 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1030_length_4644_cov_7.697973, whole genome shotgun sequence.
ACCESSION   DAWCYS010000115 DAWCYS010000000
VERSION     DAWCYS010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1030_length_4644_cov_7.697973"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1145 a         1177 c         1175 g         1147 t
ORIGIN      
        1 gccatggccg gtcgggcagg cgatcagccg gaaatcgtct ttcggaccct ggaaatgccg
       61 cgggtcagcg gcatgattat tgcaaagcag atcgaagcgg aaaaagggcc tcatccggcc
      121 atcgtctttg tcacgggcca gcgcgatttc atttctcaga cgctgcggct caatgtcctc
      181 gactatctgg tcaagccggt tacggaaggg gaagtccggc gggttgtcga aaagttctgt
      241 gccgtctata caaaaaaaga aaccccttca caggaaggaa cttctctgac ggctgccgac
      301 ggcggcgaac ggcccagtta tgcccggcgc ttttccgtcg atgaaggtga taagataaaa
      361 ctcatcgcaa cggaagatat ccgcctggtc tatgcggaaa agcgcaaggt ctatttggtt
      421 actctctcag gccggactta tcccagccat ctcagtctgg tccagtttga aaagcgtctg
      481 cctgaagaca tctttttccg ctgtcaccgc aattacatcg tcaatatcga tgaagtccag
      541 caaattgaac cgtggttcaa tcatcaatac gtcctcatcc tgaaagggat ggaagaccag
      601 cccgtgccca tcggccgatc gtatgtcaag aagctccggc agtacgtcga cttataggtt
      661 caaggcagcc ttaaagtagg ctttaaggtt ccccggcaga tgacagcatt ttgataatgc
      721 atagattgca ggatgagggt gaaaaaatgc ctggttgtta tagaataatg gcaagccata
      781 tgatataatt caaagtaaag ccttgtaaag aatatgaaag aatcgtgtaa attttgataa
      841 aggcttattc ttatcttttc gttaaggaga tcgatgatta tggcattcca cttaaagccc
      901 aaggaagaaa aattcttttc cctattggac cagcatgcga cgttgagcta tgaagcggct
      961 gaattactga gccaggctat taacggcgaa gtcgcaaaag aaacggcgct ggaaaaaatc
     1021 gacgctctgg ccgaccaggc cgatgcgctg gtcatggaaa cgatgaagcg cctccagaag
     1081 accttcatta cgcctatgga ccgtgaagat atccagcagc tgatcgatca gctcgatgca
     1141 gctgtcgaca acatcagtga aatcatggat aaaatgtgta tgtaccaagt cggcgaagct
     1201 acggatggcg ctaagcagat ggcgaccatt acggcgaaag ccatgaaaga gatccgcaaa
     1261 tccatcggct acatgaagga cctcaagaag aattacctca aagtcgaagc gcggagcaag
     1321 aaagtcttga aattggaaca ggactgcgat gatttgtacc atgaagaaat ggcgaacttg
     1381 ttcaagaact gcaaggaccc cattgaaatc atcaagtgga aggaaatcct ccagtctgtc
     1441 gaagacgtca ccgatgattg tgaagattta gtcgttacgt tcagaagggt tgtattgaag
     1501 tatgcctgag cattggttaa tttgggccat cgttttgctg gccgttctct ttgattatat
     1561 taatggcttc cacgatacgg ccaatgccat cgcgacgggc gtatcgacgc gggccattac
     1621 accgcgccac gccatcatga tggcagcgtg cctcaatttc gccggggcca tgatcagcac
     1681 cggcgtcgct aagaccattg gcggcgacct ggtcctcaat ccggctgaaa tcagcttgga
     1741 agtcatcgta gccgccctca tcggctcgat tatctggaat gtccttacct ggtattaccg
     1801 cataccgagc agttcgtccc attccctgat cggtggtgtc atcggcgccg tctgcatcag
     1861 cgtcggaccg gcagccctcg atatgggcgg tatcggcaaa atcatcttgt ctctgatcct
     1921 gtcgcctgtc gtcgccttgg ctaccgggta tttcgtcatg atcggtatcc tctggattgt
     1981 ccgcaagtat tcgccgctgg ccctgaaccg gcagttccgc aagatgcagc tcttgacggc
     2041 catcctgatg gctttttctc acggttccaa tgatgcccag aaggccatgg ggatcatcgc
     2101 cctggctctc ttgtccggcg gttatattga aactatggaa atcccgattt gggtcaagat
     2161 ggcctgcgct acgtccatgg ctttagggac ttcggccggc ggctggcgta tcatctctac
     2221 gatggggacg aagatcttca agatggaatc gatcaatggc tttgcggcgg atttgaactc
     2281 gtctatcgtc atctttacgg cgactttcct ccatctgccc gtcagtacga cccacgtcgt
     2341 atcgggctcc atcatgggca tcggttcagc cgaacgcgtc cgcgccgtcc attggggcgt
     2401 cgcccgcagt atgctcgtcg cctgggtcgt caccatcccc atcagtgccc ttatgtcggc
     2461 aatcattttc aaaattatcg attttcttta agtgaaatta gtttgtagta cggagtttgt
     2521 agtttgtaga agctggtttt aaatttttta ccatttgctg caaactaaaa actacaaact
     2581 taaaactcaa aaagaggctg tcttaatgcg acagcctctt tttgcatcag tcagtaggat
     2641 aaaagcggat gacaggctta aagtatatta ataatgtact tctgggtact ggagttacag
     2701 aattaaaact atactttttt tgtcttttat tgtataatac taaatataga caacaagggg
     2761 gtagaaatat gagattgaat caggctacgg actatgcctt ccgcatggtc ctctatttgg
     2821 cgtctctccc ggaaggcacg aagattaccg gcgcggcgct ggctgaaaaa cagaatatac
     2881 cggaacgttt tttattgaaa atcatgcgca gtctcaccaa ggctggcatc atgaagtctt
     2941 accgcggtgt cgaaggcggc ttcgccctgc agcgggctcc caaggatatc accctctttg
     3001 acatcatcga tgccgtcgaa gggcagacgg aactgcagcg ctgtctccat gatatcggct
     3061 cgtgcagccg cggctgcagc ggcatgtgct ctatttatgc cgcttttgcc gacatccagc
     3121 gcgacctggc ggcccacctg aagtccatca acttcgagac actggccaaa caggaagcca
     3181 atctgcgcat ggtccatgaa gcagaagcgc agctgacggc caaaaaagaa gcggcagaaa
     3241 agtgagcttg tagtacggag tttgtagaag atggctttaa atttaaaacc atccgctaac
     3301 cactagccac taaaaagggc ttgtcaccga cgacaagccc ttttttttat gcccaaaata
     3361 ttgtaaaata aggataccca ttttattttt attctcgttg tgtggagtga agccctatgt
     3421 acctacccct tcaaggtttc atcagcagct tccggctgct cgatatattg gatatcctgc
     3481 tcgtcgcttt tgtcctctat aaattattca tcctcatccg caatacccgg gccgtggccc
     3541 tgatcaaggg cctgctggtc ctgggcgtgg tcactgtcgt cagcaaggtc ctcgacctgc
     3601 acgtcatcaa ctggatgctg cagcagggga tgacggtcat tctcgtcgcc ctgcctgtcg
     3661 tcttccagcc ggaattgcgg cgggccctgg aacaaatcgg ccggggccgt ttctttcatt
     3721 ccggccaggt catcagcatg gaagaactgg aacggctcat cgatgaaatc gtggctatca
     3781 gtgaatccct gtcaaagacc catacaggag cgctcatcgt ctttgaacgc gaagtaggcc
     3841 ttaacgacta catcgataca ggcatcgcca tcgacggcct ggtgtcgcgg gaactgttgg
     3901 ggaatatctt cattcccaat acgccccttc acgatggcgc cgtcatcatc cgggaaaacc
     3961 ggatcatggc tgccggctgt ctgctgccgt tgacgtcgga ccggtcgctg tcgacggaat
     4021 tagggacgcg tcatcgggcg gctatcggca tcagtgaact ggccgatgcc gtcgtcgtcg
     4081 tcgtcagcga agaaacgggc tccatttctt atacctatgg cggtcatatt tatcgtcacc
     4141 tcgacggcaa tgccctgcgc aatgtcctga agacgttctt gaacaggacg cctcagggcc
     4201 tggccaatat ttttaaatgg aggccttagc ttatgaacaa gattgataaa aaatggcagc
     4261 tcaaaataat ctgcctcctc ttggctatcg tcttgtggtt cgtcatcatc aacgaacaga
     4321 atcccctcag tgaaggcagt tataccgtac cgatcactgt agagaacctc gactcccagt
     4381 atattacgtc caacgtgccc aagacggtct atgtccgcct gtcgggaccg cgcaatacga
     4441 tcatcaacgt cgggccgtcg gatatcaagg cctatatcga cttgtccagt gtccaggaag
     4501 gggaagtaga ggtcccggtc cacatcgaga tccccagcgg gacggagctg aagaagcaga
     4561 gcatgacgtc gacgaaaatc acagtttttt tttatactgt caaataattt atattgacgt
     4621 ctcttttcgt cggcaaactg gatg
//
LOCUS       DAWCYS010000116         4580 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1043_length_4580_cov_6.255249, whole genome shotgun sequence.
ACCESSION   DAWCYS010000116 DAWCYS010000000
VERSION     DAWCYS010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1043_length_4580_cov_6.255249"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1050 a         1282 c         1180 g         1068 t
ORIGIN      
        1 acatggacct gggcaacacg gtcatcccgc tgacaggcgt caccgaccag gattcgtcga
       61 ctgacgtcat gctgcgcatg cgcaacctgt ccagtgacga aagcaaggcc gtctcggaca
      121 aactgagcgg ggctttaggc ggggctgaaa tcaagcggac cgaatcggtc ggcgccgtca
      181 tcggctcgga agtcacgaaa aatgccgtca ccagcctggc tgtggctttc atcgccctgg
      241 ctgcgtacat ttccttccgc tttgaatata agatcgctat ttcggcctta gtgtcgatcc
      301 tgcacgactt gatcatggtc ctgggcttct tctcgttctt tcatctggaa atcgatgcgt
      361 ccttcctggc ggccatcctg accgtcgtcg gctactccat gaacgaagcc gtcgtcatct
      421 tcgaccgcgt ccgtgaaaac acgcggaccc accgccggac ggactcgtat gaaaaactgg
      481 cccatgacag tatcgcccag agtatccatc gcagtatcta taccctgacg acagtactct
      541 tcgcctgcgg tgccctgcac ttctttggcg gcgattcgac gaagaacttc tccctggtca
      601 tgctcatcgg cttcatcagc ggggcttatt cgtccatctg cgtcgacacg tccctgtggg
      661 tcgtatggaa gaaccatacc tctcataaac gcggtcccca cgccgtagaa gcagaaccgg
      721 cagaaacaga agaatagtaa aaggggctgt cgtattaaga cagcctcttt ttgagtatta
      781 agtttgcagt ttgtagtttt tagtgaatgg ttttttattt aaagccatct tctacaaact
      841 acaaactccg tactacaaac tataaataat ccctaaaaca aggaggaatc acgatgttta
      901 agattaatgc tttgggcaaa gcctgcccgc tcccggtcat tgaagcccac aaggctttga
      961 aggatcacga tgctgtcgaa atcactgtcg acaacgaaat cgctacggaa aacctcaaga
     1021 atttatccca ggaaatgacc tgctcctata cgatgagcca ggaatcggat acgcattaca
     1081 cggtccgcct ggtcaaggaa ggggccgatg tcagtgaaga cgaaggaccg gtcacgtcgg
     1141 gctcggacta cacggtcgtc atcaatgcac ccgtcatggg cgtcggcgac gatcagctgg
     1201 gcaagaacct gctcaagacc ttcatctaca ccctgaccga acaggatgtc ctgccgaaac
     1261 gggtcatctt ctacaacggc ggtgtcccct tggtcacgga aggttcggaa tcgctggaag
     1321 atttgaagaa cctcgaagcc cagggcgtcg aaatctacgc ctgcggtgcc tgcctcaact
     1381 actatggcct gaccgacaag gtcgccgtcg gctccattac caacatgttc cgtatcgtcg
     1441 aaatgatgcg cacggccaac cgcattgtca aaccataggc catggaagaa acgattatca
     1501 aagctgccat cctcgtatcc tttatctttt gcggctatac cttaaaacag ctgcgccttt
     1561 tcgggcgcag cacttttaat atcatttcga cgatcgtctt caacatcacc ctgccgtcgg
     1621 tcatcgtcgt caacctcaat ggcatccatt tcgacacgcg ctatctgctc atcagcctcc
     1681 tggccatcat cttcaacctg gtcatggtcg ccatgggcta tgccgccggc cggacgaagg
     1741 acgaaaaagc cttttacatg ctcaacatca acggctacaa catcggcaat ttcgccctgc
     1801 ctttcgtgtc ctacttcttc gacagcgccg ccgtcctcat cgtctgcatc ttcgatgccg
     1861 gcaactccct catgtgcctc ggcggggcct atggcctggc ccgctgtgtg cgcggtgaga
     1921 aaggggagag catcgtcatg atcctggcca agacgatttt ttcgtccatt ccggtcttat
     1981 cgtatatcac catgattacc ttgtccctgg ccggtttctc cctgccccag gtcgtcatcg
     2041 actgggccaa gatcccggcg tcggccaata ctttcttgtc catgctcatg atcggcgtcg
     2101 ccctgggctt gtccttgaaa aaggaatacc tgcacctcat ctataccgat gtgggcctgc
     2161 gcctgctggc atccgtcgtt atagcggcct tcgtctattt tgccctcgat tatcccatgg
     2221 ccatcaagaa agtcatcatg gtcctggtct ttgcacccgt tgctggcatg gcctgctact
     2281 acacggccaa gatgaagctt aaaatcgaag tggctgcctg catcagctca ttttatatcc
     2341 tcttgtccat cgtcgtcatg tcggccatga tcatcgccct gcagtccgta tgagcagtcg
     2401 gccagtaatt cttcttccag gtcggccgga atcgtcgacg ggctgcgcgt gatgtagtag
     2461 ctgtcgactt tgggcggctc gaaatcgagg aggggaatgg cccgcaggcg cttttcggcc
     2521 aggtcctctt cgatgaggct cttgggcagg aaggtatagc cgatgccgtc ggccacgtac
     2581 tggggcagct tggtgctgtt gtcgatttcc agggggaagc ggtgatggcg tgggaacagg
     2641 tcctggatgt agaggcccac gccctggagg gcgaaattgc agaagaggta gtcgattttc
     2701 tgcaggtcgt ccttggaaat gccgttggca tattccgtat tggctgcttg gcagaccagg
     2761 accagcgagt ctgtccggta ggggcggcag atatagccat cgcggaacat ggcgctgaag
     2821 gaaaagatcg cgtccagcgt cccgtcctgg agctggtgca tcatggccgt cgtatgaccg
     2881 atggtgacgt gcagggatag gtccttcttc tctttgagga agctgcggat gcggcgctgg
     2941 aggtgacatt cgtaaatggt gttggtcgtg ccgatgctga ttttctgggc atacgtcggt
     3001 gacgactgga tgtcctgcag agctgccagt tccaacgaga cgaagcgctg ggcatagtcc
     3061 aggaatttct ggcccgacgg cgtcaggtcg acttgcttgt ggctgcggat gaataagggc
     3121 acgcccagtt ccatttccag gtcgcggatg cggttggtga ccgtcgattg tgcgacgaag
     3181 agctgttgtg ccgttttggt gaagttcttg atttgtgcca gggcgatgaa cgtccggagt
     3241 gtcgttgtgt ccatggccgg acctccttca gataatttga tttaatttga ttatcgaatg
     3301 aaataaatta aaataatcta tttcacaaat tgtctacttg tattatataa taacccatgt
     3361 agagaaacaa tgaattcaat tacaagaaac tggagctgaa ataggatgaa caaaaaaact
     3421 gtccccttta acgaagaaca aatccgttct atcattgccg aacacccgac accgttccat
     3481 atctacgacg aaaaagccat cgtcgataat gtccgtaaat tgattaaagc ctttgactgg
     3541 gcttatgact tcaaagaata ttttgccgtc aaggctacgc cgaacccgta catcatgcgc
     3601 ctcttgcaga aagaaggcgt cggtgcagac tgcagttccc tggcagaact ggaactgtgc
     3661 gacaaagtcg gcatcaaagg ccatgacatc gtcttctcgt ccaatgacac gccctatgct
     3721 gaattcaagc gggccatgga actgggcgcc ctggtcaacc tcgacgatat ttccatgatc
     3781 gaattcatgg aagaacgcgg tcccctgccg gaatggatct gcgcccgcta caatccgggc
     3841 ccgctcctca aaggcggcaa cgacatcatc ggtacgccgg aagaagccaa atacggcatg
     3901 acccgcgaac aggtcatcga cgccttccgc atcctcaagg ataaaggcgt caaacacttc
     3961 ggcctgcaca ccatggtcgt ctccaacgaa ctccacgtca acggcctgat caacacggcc
     4021 aagatgatgt ttgaactggc tgtcgaagta cagaacatgc tgggtatcaa tatcgaattc
     4081 ctcgacttcg gcggcggcat cggccttcct taccgtccgg aagaatcgtt catcgactat
     4141 gacgaactca gtgcgggcat caagaaacac tttgaagaaa tcatggaacc ggctggcctc
     4201 ggcaaatgcc gcatctcctt tgaatgtggc cgtgccatca ccggccctta cggctacctg
     4261 gtcacgacgg ctatccatca caagcacatc tggaaggaat acatcggcgt cgatgcctgc
     4321 atggccaacc tcatgcgtcc gggcatgtac ggttcgtacc atcacatcac ggtcatgggc
     4381 aaggaaaatg caccgaaaga ccacgtctat gacgtcgccg gttcgctctg cgaaaactgc
     4441 gataaattcg ccatcgaccg cagcctgccg gaaatcgacg acggcgacat cctggtcatc
     4501 cacgacacag gcgcacacgg ccattccatg ggcttcaact acaacggcaa gctccgttcg
     4561 gcggaactcc tgctccatgc
//
LOCUS       DAWCYS010000117         4550 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1048_length_4550_cov_4.805117, whole genome shotgun sequence.
ACCESSION   DAWCYS010000117 DAWCYS010000000
VERSION     DAWCYS010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_4550_cov_4.805117"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1686..1695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          917 a         1248 c         1291 g         1084 t
ORIGIN      
        1 ctatgatatt tttgcataca gaaaaagagc ctgtgcacga ttacgtgcga cagaccctct
       61 ttttgagtat taagtttgca gtttatagtt tttagtaaaa agcttactgc tgcgcacggc
      121 gcattgcttt tttacgatcg tgtttgtcga ggatggcttt acggatacgg atgctcttcg
      181 gcgtaacttc tacgagttcg tcgtcgttga tccattccag ggatttttcc aagcccattt
      241 cctgcggcgg cgtgaggcgg agtgcttcgt ccgatgcgga ggaacgggtg ttggtcaagt
      301 gtttcttctt gcacgggttg acgtcgatgt cgacgtcgcg gttgctttcg ccgacgacct
      361 ggccaagata gatttcttca cccggtttga tgaacatcgt gccgcggtcc tgcaagttga
      421 agatgccata gcttgtcgtc gtgcccgatt caaaggcaac taagctgccg cgggtacggc
      481 ccgggatttc gcctttccag ggagcatagc cgtggaagac gtggtacatg atgccattgc
      541 ccttggtagc ggtgaggaat tcgctgcgga agccgatgag gccgcgggcc ggaatgatga
      601 attccaggcg ggtgtaaccg gaaatatcgg tcatgttgac catttcggct ttacgggtac
      661 cgagtttttc catgacagcg cccatgaatt caggcggaac gtcgatggtg agggcttcca
      721 gcggttcgca tttctggccg ttgatttcct tggtgatgac gctcggtttg ccgatctgga
      781 gttcataacc ctgacggcgc atttcttcga tgaggacagc gatgtggagt tcgccacggc
      841 ccgatacttt gaaggcatcg gcactgtcgg tttcttcgac gcgcagggat acgttggtct
      901 ggacttcgcg gaacaggcgg tcgcggaggt gacggctggt gacgtattcc ccttcctggc
      961 cggcaaaggg gctgtcgttg acgtagaaaa tcatggacag cgtcggttcg tcgatgttga
     1021 ttttcggcaa agcttccggg ttctgcgggt ccgtaacggt ttcgccgatc ttgaggtccg
     1081 ggatgccgac gatgcaggcg atttcgccca tagcggcttc atcctgttcg acacgtttca
     1141 ggccttcata ggtatagacg cggccaatct tagctttgcg ctggctttcg ccgtcagtga
     1201 cgacgacagc ctggttcgga tacatcttgc cgcggacaat acggccgata gcgattttac
     1261 cgacataatc atcgtagtcc aaggtcgtga ccatgaactg cagcgggcct tcagcgtcgc
     1321 ctttcgggca cgggcagtat ttgatgatgg tcttgaacaa cggttcgagg ttgtcgctgt
     1381 cgtcatccat ggagtattta gcaatgccgt cacggccgtt tgcatagacg acagggaaat
     1441 cgagctgttc gtccgtagcg tcgagttcca tgaacaattc gagtacttcg tcttcgactt
     1501 cagcgacacg ggcaccgggt ttgtcgatct tgttgatgac gacgatcggt ttcaatttct
     1561 gttccaaagc cttgcgcaga acgtatttcg tctgcggcat cgggccttca aaagcatcga
     1621 cgaggagcag tacgccgtct accatgttca ggatacgttc cacttcgccg ccgaagtcag
     1681 catgcnnnnn nnnnncttcg agcatgacag atgtattttt ggacaagatt gtaatgccgc
     1741 gttcacgttc aatatcccca gagtccatga cacgttcttc gactttttcg ttagaacgga
     1801 aaacatggct ctgtttgagc atggcatcga ccagtgtcgt tttgccatgg tcgacgtggg
     1861 caataattgc aatattacga atatctttgc gttccatcta atgtcctctc tctcattaac
     1921 aaatattaga ttgtatataa ctaggttagt atagcatgga ttgctcagaa atgcaaagcc
     1981 gcctgctgaa tttattgtca acaggcggta aaatatcaga tagtttctgc ctaagcttta
     2041 ttcttctgga gcatgatctt taagatttca tcgtctgtct gctgcatccc gcgggaagcc
     2101 aggaggccga cgttcttcag acattcttcg gctgcattgg ccactaagcc gtccgtaccc
     2161 tggacgcgca cgtcattgag ggccaggagg accgcttcat aggccgcatg gaccgacgcc
     2221 gacaccttca tggaacagct gttggccgcg ccgtcgcaga ccatgccgac gatggacccg
     2281 gacatggtgg ccagggcctt ttcgatgacc tgcagcgggt ccttttcggc cagcagccag
     2341 gccatgccgg cagccgcccc catggaagcc gtgacggtag cacagaaagc cgataatttc
     2401 ggcagatagc cgtgggcata aatggcgacc atgctggaca aggccagtgc ccgcagccgc
     2461 cgttccggcg tgacaccgag cgaatcggcg acgaccgtca cgggttctgt cgcggcgatg
     2521 ccctgattgc cggaaccgga attggtcata gccgggaaag ccgccccgcc catacgggca
     2581 tcggaagcgg cgaccgtgcg gatctggatg cgatgggaca aatcatcgga caataagtct
     2641 ttatcgacca tctgctgcaa ggtatagccg atcttcaggc catatttccc cgtcaggccc
     2701 tcctgcgaca gctggtcgtt gagccgttcc gcctcctgca ggaaggcgat gtcggccagc
     2761 ggcacgtgct cggcaaaatc gacgatctcc gccagggtca gcgtatggag aaaagcgatt
     2821 ttcgggtctt ccccgctccc cgcttccgtc cgctggtcca agaggacctg gccatccttt
     2881 tccacgaaga cgatgttcgt atggtcgtcg gtgatgacga cgcggacgcg gtggttttcg
     2941 ccatagacga cggcttcggc atagaggaca tgaggcgtat cttcccggac ggacacagtg
     3001 actttaccat cggcgacgta ctgtttgccc aaggcgacga cgtctggcgt caaggccttc
     3061 aggacctgta atcccgctcc ggcgtccccg ccgagggcgc cgacggcagc ggcaatatag
     3121 agccccggcg tccccgtgcc cggtacggtg acgcccatgc cgttcttcat cagattcgcc
     3181 gacacgcggg catcgacgaa gcgcacggct tcgcccaatt cccgggccgc cgtcgccgcc
     3241 gcataagcca gggcaatcgg ttccgtacag cccgtcgccg gcgtcacatc ctgcttgatg
     3301 atgccgatca tcttgttcca catagcattt ttatccataa ttatccctgc tttctcctat
     3361 aaataggtat cataacgtaa atcgtatcac gtatctttct aatatcatca tagcatgatt
     3421 actatgagaa aacaatatgg atatgaggca gttcgcagac cgcagttggc acttcgccgc
     3481 tcttttaggt ccttcgcata ttgcaagaaa gaaaaaaaga agctgtcgca cgaagccaaa
     3541 atggcttaat gcgacagccc ctttttctgt aggggcgcca cgcagggcgc ccgctccaaa
     3601 atatgtacga tttcccagcc agtttgctag aaaaatcacc caaatcccta acgggccgcc
     3661 cacgggcggc ccctacaaac tccatactag tggatggagt taaattttta gtcttcgccg
     3721 atgatgcgga cttccggttc caggtggaca ccgtgagcct tttcgacgcg tttctggact
     3781 tcgtggatga cgttgaggac atcccgggcc gatgcgtgcc ccgtgttgac gacgaagccg
     3841 gcgtgtttca tcgagacctg ggcatcgccg caggagaggc ctttgaggcc cgtctggtcg
     3901 atgagggtcc cggcatagta tcccggcggc cgtttgaagg tactgccggc gctgtgcatt
     3961 tccaaaggct gcttgctgcg gcggcggttc atgaggtcat ccatgcgggc ctggatttcc
     4021 ttggggtcgc cgctgcgcag ggtcaattcg acttcgacga cgaattcctt ggatttctgg
     4081 aaacggctct ggcgataaga aaagcccagg ttcggtgcat catacgtatg gacgccgccc
     4141 gtactgttga ccgtgcggac ccgcgatacg acgtggctca tttccccgtc gtaggccccg
     4201 gcgttcatga agacggcacc gcccaaggtc ccagggatac cgcaggcgaa ttcgatgcct
     4261 gtcaggctgt tttcctgggc gaactgacag atgtccttca gcatgaaacc ggcactggcc
     4321 atgatcttcc ggtcgtggca ggccaatact ttcttcatcg tgttcagctg gatgacgacg
     4381 ccgcggatac ccccgtcgcg gacgaggacg ttcgagccgc cgccgaggac ggtgaccggc
     4441 aggtccagcc gccgggctgc ccggatggcc agcgacattt ccttgaccga ttccggcaag
     4501 atcaggacgt cagccggacc gccgacggca aaggtcgtgt gttcgctcat
//
LOCUS       DAWCYS010000118         4549 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1049_length_4549_cov_7.365599, whole genome shotgun sequence.
ACCESSION   DAWCYS010000118 DAWCYS010000000
VERSION     DAWCYS010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1049_length_4549_cov_7.365599"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1432..1441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1098 a         1188 c         1091 g         1162 t
ORIGIN      
        1 ccattcgtcc gcatcgcagc aggccttcaa ggctgagact tgctccaaat catcctggtt
       61 gaaatgctga atattttccc ataaagcctg tttgtatccc tcatcgtctc ccaatgcttg
      121 gtaggcatga atgagcttgg tgctgtaatc ctgctggcaa taatccttat cgtcatcgat
      181 ggcctgagaa tcacggagca gtttgacggc tctttggtaa tcattgtctt taaacgcatc
      241 ggaaatcagc atgttccgta cctgtctgtt ttcataatgg gcctggatat attgctcaat
      301 atcggtttgc ggatagccta attgctgcat cgtatgaatc cgcatgacca gattggtgcc
      361 ataggtatag ccgtaattcc agtcgttggg gtcggatttc gccgattcat gatgctcgat
      421 ttccgcatcc agccgtgcaa gcttccattt caaggacgcc gggtcatcaa aatcattgta
      481 cagcacctct tcgtaatagc cgtatagctc attgcccttg tttttgattg catccatctg
      541 ctgtataaac cattgatgca gcttcggcac gtcttccggt ttggcatgac caatgatatc
      601 ggcccagtaa gttcgacatt catccagaca gagctgctgg tcatcttcat cttcaaaaga
      661 tatatccgcc acattggacg ccatgatatt gaccagttcc aatgcttcca tcgtacaaga
      721 gcgcttaatc agcggcggta tggtgctgct caaataatca aggaccttgt cagcgaaagc
      781 cttactggca ttatattcaa tatatcccaa gaggtcgtaa ggagccaata cccggtctat
      841 ttcgcgttca tatcgatgca tttcctgagc agacggtttg cccctggaaa aatctagtac
      901 gattttcttc tgcaaatccc gattatcttc ggctaattga aagacaaagt tacgcaaatc
      961 tgattcagac aggcgctgca agatatcctg ccacgatgct tttttctgtc cagcatcagc
     1021 cgcttcatcc agttcttcgt cttcagcggc cagcagtacg gcgaccatgt gcttgcaata
     1081 ttctccctgt tcggcatagg gacaggtgca gtccatccct tcgacctggc cgtcgtccaa
     1141 atcgatagtg acttcatata tatcactgcc atagacgtcg gccgttatct gcttctccgt
     1201 gcacttcaga tcggagacgt cgccttcttc gtaatagcgt tcgccacgct tgtaaatttt
     1261 agatgaaaag aagtctctcc aattccctaa atccattttt ctcacccctg tgcattgtga
     1321 ttttctttct tgatacggtt caagtagcgg taaatcgtcg gttccgaaac gcccaggatc
     1381 tgggccaggg catagacgct gcctttgagg acgaagaggc cgttcttgta gnnnnnnnnn
     1441 nttcttgtag aggtcttcga cgacagccag gcgttctgcc tgggataggc gtttggcctc
     1501 ggtcccgtaa ggggccaggg tccgctggat gagatgctgg atggcatcgt ctacggtcgg
     1561 atacatgctt tctgagattt cgacgctcag gaaatggtca tcttccttct tagcgttatc
     1621 ctgaggcatg ccgtcgatat gcttccccgg gtccatcaag atgaaggcat ccagttgttt
     1681 ccgcacgtcc agatagcgct ggacatcgta attcagacag agggccccga taatattatc
     1741 ctcctcatca tggataaaaa aagtcgaaga ccgcagggta tggccgttga cgctcttccc
     1801 gtagtaattg gtcatatatt cctggtccct tttcttaccg acctgcatga gtttcaaaat
     1861 aaaatccgtc gccggcgccc ccactttccg gctgctgatg tcgccattgg caatggcgat
     1921 gaccgattca tcgggataac gcagatcatg aaggacgact tcacagtcct tgcccaaggc
     1981 agctcccaaa aaaggcacga tacgtttata catctctaaa atggacatga cgtcacttcc
     2041 cctatgtagt ataagaaaat tttatcacaa tgagaatgta tagggcaaga aaaaagaggc
     2101 cgccccataa agacagtctc tttttacgtt aatcgtaggt tgttcatcgt ttgtcgtacg
     2161 ggattaaaaa tatcacccca tcgcaatgac cgatgaacta cgaacgcaaa acgtttaagc
     2221 ggctagcagt tagtggttag ccgctggttt taaatttaaa gccattttct acaaactaca
     2281 aactccgaac tccaaactaa tacttacact tctgcaatga cttcgatttc gcagaggaaa
     2341 tgagccggaa gttctttgac ggctacgcag ctgcgggccg gtttgctgat gaaagctttt
     2401 tcgtaaatgg cgttgaaagc ggcaaagtct ttcatgtcat agaggaagca cgtcgtcttg
     2461 acgactttcg agaagtccga gccggctgct tcgaggatag cggcaatatt tttgacagcc
     2521 tgttccgtct gggcttcaat gccttcctgg agtttgcccg tctgcggatc gacaccagcc
     2581 gtcccggaaa tatacaacat accattcgat accatagcct gcgaataagg accgatagct
     2641 ttcggtgcat taggggaaac aattacgttc atggaaatac ctcctgtaag atagctttat
     2701 ttttactaat aaaatattat cgcgataata attgatttgc aagcgttatt tcgacatttt
     2761 ttatccgaaa atgcgtcata gcccccttac aatgttgaca tatgtcatct ataaatctat
     2821 aataaaaggc gtaaaggagg gggtactatg ccgagaccac cgcggtgccg acgggtctgt
     2881 tcgttgcctg tttcggcggc tttcgagcct atagatggcg cacagtcaga cgatgtgatt
     2941 gtcatgggca ttgacgaata cgaagtcatc cgcctcatcg actgggtcgg cctgacgcag
     3001 gaacagtgtg ccgctcagat caatgtggcg cgcacgacgg taaccggcat ttatgattcg
     3061 gcccgacaca agttggccga cgccctcatc catggcaaac gcctcgtcat cgaaggcggc
     3121 catatccaag tttgtgaacg ttccggcacg tgttgtcata cctgctgccg cgccggaaaa
     3181 aagaatacaa aggagaatga tcccaatgag tgaaaattgt acacacgact gttccagctg
     3241 cggcagctcc tgcgctgaac gcacacagcc ccagagcctg caggccgccc ctcatgccgg
     3301 ctcgcacatc aagcatgtca tcgctgtcgt cagcggcaaa ggcggcgtcg gtaaatccct
     3361 ggtaacgtcc cttatggccg ttgaaatgca gcgccgcggc ttcaagacgg ctatcctcga
     3421 cgccgatatc acgggcccgt cgattccgaa agtcttcggc ctgaccgacc acgctacggg
     3481 cgatgaaaac ggtatctatc cggtaacgac caagacgggc atcaaggtca tgtccatgaa
     3541 cctcctgctc gaagacgcag cggctcctgt cgtatggcgc ggcccggtca tttccggtgc
     3601 ggtaaagcag ttctggaccg atgtcatctg gggcgatatc gactacatgt tcgtcgacat
     3661 gccgccggga acgggcgacg tcccgctgac ggtcttccag tccctgccaa tggacggcat
     3721 cctggtcgtc acatcgccgc aggaactcgt ttccatgatc gtcgaaaaag cactgaacat
     3781 gtcggaaatg atgcacgtcc cagtcctggg cctcatcgag aacatgagct attttgaatg
     3841 tcctcattgt catgaaaaac tgtccatctt cggcaagagc cacgtcgaag aatcggcaca
     3901 gaaatatgat atcccccata cggcaaaact gccgatcgac ccggaattcg cagcccactg
     3961 cgataaaggc gatatcgaag gctatgaagc cagctatatg gcagataccg cttccttcct
     4021 ggaaggcgtg ttagggaaat aataaaaagg cgctgtcgca cgaagacagc gccttttctt
     4081 gtaggggcgc cacgtggggc gcccgctctg ggatatgtac gatttcctgc cctgtttgct
     4141 agaagaatca tccaaatccc taacgggtcg cccacgtggc gccccctaca tactccgaat
     4201 tacaaactat aaaagagcaa cctttcagtc agcttcgctg acagctctcc tatgaagaga
     4261 gccactgcgg gccacgagcc actaattatt tcacattttt cgtagcatcg atgccgaggg
     4321 ctctcatgat gccaaagaat acttcggtgt tgtccatgac gccctggaag tattccgagc
     4381 caggaccttc agcgttgagc ggtacgtcgt cggcagcatg gcattcgtca ccgatggtat
     4441 cgggaatgtt ggccggtacg atttcccacg gatcgccgtc gtgattgagg gccagattag
     4501 cttgttcgac gactgtcgat accagatgga attcggtcgt atcgccagc
//
LOCUS       DAWCYS010000119         4496 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1066_length_4496_cov_5.629588, whole genome shotgun sequence.
ACCESSION   DAWCYS010000119 DAWCYS010000000
VERSION     DAWCYS010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_4496_cov_5.629588"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          895 a         1163 c         1369 g         1069 t
ORIGIN      
        1 cccggcgtgt cgaggaagag gatctgactt tcttcgtccg tatggatgga gaggatgcgg
       61 ttgcgcgtcg tctgtgcctt atcggagacg atcgagactt tatgcttgat gatgcggttg
      121 atgagtgtcg acttgccaac attcgggcgg ccgacgacgg cgacgaaacc cgatttaaat
      181 gattccatag atatcgtaat tcctccctta taattcttcg ccttcgcgga catagcccag
      241 gcgctgtaaa atgaattctt cttctttgcg catttctgct ttatcgtccg gttccatgtg
      301 gtcatagccg aggatatgca gcatgccgtg gatggtcaga taggccagtt cccgttcaaa
      361 ggaatgaccg tattcgtcgg cctgttcctg gactttttcc agagaaatga tgatatcccc
      421 caagagggcc gtatcgggac cgtcatagcc gtcttcctcc ccttcgttga gggcaaagga
      481 caggacgtcc gtcggccggt cgatgttgcg gtactgtttg ttcaatgtat ggatatagtc
      541 gttatggcac agaaggatgc tgatttcagc cttcggcccc aggtcgtaga ccagggcggc
      601 ttcttcacag acgcggtcga tgactttttc atattcatcg tgctggaacg acggatcttc
      661 gtaattgatg ttgatattca tggcaattcc cccaatctag tgatcgtgat gataataagt
      721 ttcgtaggcc ctgatgatgc ggcctaccat atcgtgacgg acgacgtctt catcgctgaa
      781 gtagaccatg ccgacgccgc ggacgtcttt gagtatcttt tcggcttccg tcaggcccga
      841 catgcgccgg tcgacgaggt cgatctgggt cgcatcgccg ttgacgacga cccgcgagtg
      901 gttgcccata cgggtgagga acatcttcat ctgttcccgc gtcgtgttct gggcttcgtc
      961 gaggatgatg aacgattctt ccagggtccg gccgcgcatg taggccagtg gcgccacgtc
     1021 gctgatgttg cggtcgatca tcttctggaa ctggtcatag ccgaacatgt cggccagggc
     1081 gtcgtagagc gggcgcaggt acgggtcgat cttgtcctgc aggtcaccgg gcaggaagcc
     1141 cagtttttcc ccggcttcga cagccggccg ggtcacgatg atccgttcga cttcgcggtt
     1201 cttgagataa aaagccgcca gggctacggc caggtaggtc ttgcccgtcc cggtcggtcc
     1261 gatgccgaag gtgaccaggt tcttgcggat ggaatctacg tagcggttca gcccccgcgt
     1321 cttgggcttg atgagacggc cccgggcaga aatgccgacc gtatcttcat agaggttgtg
     1381 cagggcctcc gattggcctt ctttgatgag tctggccgaa tagcggacgt gctgcgtcgt
     1441 cagataggta ttttcccgga acaagtagcg caggacgcgg aagacttcgg tcacataggc
     1501 cacatcctct tcgctgccga cgatcatggc cgtatcgccg cggacggcaa tgcggcagtc
     1561 cgggaattcg cgctgcatcg tcttgaggaa ttcgtctttc atgcccagga cggcccgggc
     1621 ttcacgcaaa tcggaaaatt gcagtttctt ttctaccaaa gtgagatact cctttatttc
     1681 atgaatttac ggacggcctt gccgtgcagg tagtcacagc gggctccctg gcaggtgccg
     1741 cgtttttcca gttcttcatc gatcatatcg cggatgcgcc accatcccat gccccagttg
     1801 gcgaagtccg tcgcttcttt cggcgcccgg ccgatgagac gctggaagac cgtatcgggc
     1861 cggctgtatt cgaggaaggc cacgacgcgg cggacgtact cttcgcaggt ccagagcttg
     1921 atctttccct gggcatacca ttcggccatg gccgtgttct taatgatgta cagggcgtgg
     1981 agcttgactt cgtcagagcc gagggcggca atgatcttgg ctgtctcgac ggtgtcttcc
     2041 atggtgtccc agggcaggtc gaggatgaca tgcgtacagg tccggaaagg atactcccgg
     2101 agcatgacca tggcgtcgat gtattcggca acggtatggc cgcggttgat cttggccaag
     2161 gtcttatagt tgaccgtctg caggcccagt tcgacgagga tatcgacgcc gtacctttct
     2221 ttgacgtcag ccatgatatc gagataggtc tcgttgagac agtccggccg ggtcgctacg
     2281 gccagggcca cgatatcggg ctggcaggcg gcggcgaggt aatcccggaa acgctctggc
     2341 ggcaggtagg tattggtgaa attctggaaa taggcgatga atttattggc cttgtacttg
     2401 ggcttgatgt gagccatgtt ctgctcgatc tgctggccaa tggtcaccgt cgccggcagg
     2461 ttctcgtagc cggcgccgat ggaaccgcag aaggcacagc cccggacgcc cagggtcccg
     2521 tcgcggttgg gacaggtatc ggggatgctg ataggcagtt tatagacttt ttcgccatat
     2581 gtttctttta aatacgtcga gtagacgcgg taacgttcgt tcacgatggt tctcctttga
     2641 ccgggatggc gcaccattcg gcccggtctt tctttttttc agccagcata taggccgcgc
     2701 cttccgggac gggcccgtag cggcgcagtt cccctttcag ggccgggctg gcaggccaga
     2761 tgcgatagcg catcttgatg tcgcgccagt tggtgaaatg atagtccctg gcaaattggc
     2821 ggagccaggc ttcgtcgcgg aagatggcgt cggtctggct gcgcggcgct tcgcaccagc
     2881 cgaggaaatc gggatggagg cggaagcgga aaaaggtcag catatccagg gccagcaggt
     2941 ctttggccaa gcgctgctgg tcttctgtca ggggcaggat atcttcgatg cagtggcaga
     3001 ggaaatgaca caaggactca tcggacaggg cctttgggtc attatgcagg acgagccact
     3061 cgtctgtcaa ggcctcgtag agtttcatat aatccggagc atagcggcgg ccgagccagt
     3121 tgaagaaata agtgaatttc ccggaattat aagtccgctc gaagacgtct tccaggacct
     3181 tcatataggc gactttttca taaggcagcc acgtcgtcgc caggacttca tagggcccct
     3241 gcgggtcgta ccggtaatcg tcaggatagt cgcggcggat gccggagccc ttgaggagct
     3301 tcaagaaacc gatctgcaat ttatgggggt gcagggcata gatatcgttg aaggactggc
     3361 gcagatggcc gtagtcttca taaggcaggc cgacgatgag gtctaaatgg agatgcatcg
     3421 tccccaaggc gacaaggcgt tccatgatat gcgtaatgcg cggccagtca ttatgacggt
     3481 ggatggcctg gagcgtcggc tcatacgtcg tctggatgcc catttccagc tggatccgtc
     3541 ctttcggtgc ctgggccagg aggtcgatgt cgtcatcggt aaggacgccg ggctcgattt
     3601 ccaggtggaa attgatgggc ttatcgacga caatcatgta cgctatgagc ggccggtgat
     3661 gggccgggct gcagttgaaa gtgcggtcga cgaatttgac ctgatggacg ccggcatcga
     3721 cgaaagcctg cagttccttc aggaccagcg ggaccgagcg gaagcggacg cggtcgttca
     3781 tgcccgacag acagtactgg cagtggaaag gacagccccg ggagctttca taatacatga
     3841 tgcggtggtc cagttcgcgg aagtcctgat gctcgtaagg aaaaggcagg ctgtccaggt
     3901 cttctacttc ctggaaggtc tcgtcgccct ggatgacgcc gctgacgcgg ccgaggacgc
     3961 cggggacttc tgtcccatcg cggccggctg ccagggattt caggaaagcc ggtaccatct
     4021 cttccccttc accttggagg acgtaatcaa tatgctcata atcttccagt atctgccggg
     4081 ccgtatagga tacttccggg ccgccgacga agaccttggt ctggggaaag acctgtttca
     4141 ccaggtcgat gagatgacag gtcatttcga tattccagat atagcaggaa aagccgatga
     4201 cgtcggcctc gaacgaggcg atgtggcgca ggatgtcata gctgttctga ttgatcgtat
     4261 attcggctac ggacacggcg acgccctggt ggtcacacgc cgtcgacagg cagcgcaggg
     4321 ccagggacga ttggatgaat ttggaattca agatggtaag cagtgttttc atgaaagcct
     4381 ctctttaaat ttttatatgt attaagtata ccgcaaggac ggcgttctgt catgtctttt
     4441 ccgccgctgg gcggggacag ccttttcgcc tgcccctttt tatgataaga tgagat
//
LOCUS       DAWCYS010000120         4495 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1067_length_4495_cov_4.643243, whole genome shotgun sequence.
ACCESSION   DAWCYS010000120 DAWCYS010000000
VERSION     DAWCYS010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_4495_cov_4.643243"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          968 a         1135 c         1221 g         1171 t
ORIGIN      
        1 tcccacagaa ccgccaaaac ccatcgtaaa agtccccatc gaagaacgcg tgaaagtcct
       61 tcagtaagaa aaaagctgcc atacgaaggc agcttttttc gagtgttaag caagcgataa
      121 acttgtaata tgaaaaaaac cgcgtacttg tggtacacgg ccttcttcaa acactaaaac
      181 tagaggagag ccgatgtccc accaccgagc tagctctatt ctcattctat catgaatgta
      241 gataaatcaa gtggattctc gtcctgatta tccaagtaaa tcctgttcgt ctttgtggcg
      301 gcggaaccag gcgtcggcgt aggagcaact ggtggccgtt tttttatggc gccgccgtat
      361 ttcgtcagct gcggctttga cgagtttgcc agcggcacct tggccgcgca ggctgtcgtc
      421 gacaaaggtg tggacgatgt cgtaattgcc gtcttcccgt tcgacgaagt ccacttcagc
      481 tacgaccgtt ccagcttcat tttccatcca gattttattg ggtccatatt gtaattccat
      541 ggtaatttct cctttacatc gtaatttcaa caatctgtcc attatggacc gggcggctgc
      601 ggcggaaagc ggccatgtat tcttctacag gcctgcccgg attttcatag accaagagca
      661 ggttgcggat aaactgggcg tgcgtaaaga ccaggatgaa ttgctccggc cgccgctgca
      721 gcagagataa ggtccgttcg attcgcccga tgagctggcg ataactttcg gccccttcgc
      781 cgtcgacata gtccgggtcg agtttgcgcc aataattgat gacccgcggc cgccgctgct
      841 gcgacgtcgt cccgacacac gtcgccggtg ccaaataaac aaattcatgg acacagtccc
      901 aaatccccgt caccgtatca ggaaaccgcc ctattgtaca catggccgtc tgctgcgccc
      961 gaagataagg cgacgtgaca atcaggtccg gtttcttagg aatctgtaaa gccaaggcct
     1021 gcgcctgctt gagccccaat tccgacaagg gaatattgcg atgatcctgc gtcgcttcgc
     1081 cggcattggc aatactttcg ccatgacgga tgagccatat tctcttcaca ttatcacctg
     1141 cttttttctt ttattttatc acaaaaaaag gagaatttga aagacaactt ctccggcctg
     1201 gcgaccacct cccctatgag gggaggcttt cccaatggct ctccacatag gagagctgtc
     1261 agcgcaggct gactgagagg ttgcttttta atcaccagga acagccttta tgctcactga
     1321 cgtattgatg aatagtttgg caaactccgc taaaattttt atggatatcc aaattggtaa
     1381 aacggatgac cgtcaatcca tagaattcca atacagctgt ccgttcctga tctgacttca
     1441 taccggcttc atcatagtgc tgcccaccgt ctaattcaat gatgagccct gcttctttgc
     1501 aatagaaatc ggcaatataa ttaccaattc gtcgttgttt ataaaatttc actggatacg
     1561 tccgtaaaaa atcataccat aagtgacgtt cttctttggt catatttttc cttaataaac
     1621 gggcaaatga tatattttct ttagaatagg gaagcgtttt catagaacct ccttttaaaa
     1681 tatattgaac ttcaacctct ccggcctagc ggccacctcc cctatgagga gaggctttcc
     1741 caatggctct cctcatagga gagctgtcag cgcaagctga ctgagaggtt gctttttgat
     1801 catcgataga cattggtgaa cttcagcctc ttcggcctga cggccacctc ccctatgagg
     1861 ggaggcttgg tttccgaggc gatgtccttc ttttgagtct gtcgcggctc tgccgcatct
     1921 gagtccgagt gtgcgaaaac agggccgcct ttgggggcag ccctgttttc gggtgtgtat
     1981 atggcattag cgtgttttac ctatttatca gccaattcct tataagtttc gtatttcttc
     2041 ttagcgaatt cttcggcctt ggcaaagagt tcttcggcga tatccgggaa ggttctcgtc
     2101 agggcagcat atctcgtttc acccatgagg tattcgcgga aggattgctg cggttctttg
     2161 gagtcgagga tgaaggggtt cttgccttct tctttgagca gcgggttgta gcgatagagg
     2221 tgccagtaac cgcagtctac ggcgcgtttc atttcgtgct gggcgttgcc catgccgcct
     2281 ttgatgccat ggttgatgca cggtgcatag gcgatgatga tggacgggcc attgtaggct
     2341 tctgcttctt tgatggcttt gatgagctgg ttcgggtcag cgccgagggc gacctgggcg
     2401 atatagatat ggccataggt catgagcagg cggcccaagt ctttcttgtt gaacttgcgg
     2461 cccgatgcag cgaactgagc gacagcaccg attggcgtag ctttcgacga ctggccgcca
     2521 gtgttggagt agacttccgt gtcgacgagc atgatattga cgttggcgtt catagccagg
     2581 acgtggtcca gaccgccgta gccgatatca taagcccagc cgtcgccgcc atacatccag
     2641 atggtcttct tgatgatgtg ttctttatga gccaggattt ctttctgcaa gtctgcagcc
     2701 cggccggtga gatgagcagc catcaattct ttgatgtatg tccggctgat gccttctgtg
     2761 acttcgcctt cgttagcgac gccgatggaa tcgatccagg ctttgatggc gtcgtggagg
     2821 gcaccgtcag tcaggcccag gagttcttcc gatttaatgc ggatagcatc gcgctgctgt
     2881 tccatggaca gggacatgcc gagggcgaat tctgcgttgt cttcaaagac cgagttcgac
     2941 caggccggcc cgaagccttc acagttcgtc gtgtacggga tgctgggcat tgccgagccc
     3001 caagcctggg tacagcccgt agcattggcg acgatcatgc gcggcccgaa gagctgggtc
     3061 aggatcttca tgtaagccgt ttccgtacag cctgcacatg cgccgttgaa ttcgaggagc
     3121 ggctgatgga actggctgcc tttgacggag aatttatcga gagtcgtatg tttttcaggg
     3181 agtgtctggg cataatccca gtcgacgctt tcatgacgct gcgtttccag cggcttcatg
     3241 gtcagggcct gagccgggca ggcattgacg caggcactgc agctgtagca gtcgagcggc
     3301 gaaacctgga tgcggaattc atagtcttcc aggcccttgc cgatggcttt cttggtcttg
     3361 aagtcagccg gagccttggc tttttcgtca gccgttacca gatacggacg gatagcggcg
     3421 tgcgggcaga ccagggagca gcggttacac tggacgcatt tttccaggtc ccattcagga
     3481 acggtcgatg cgatgccgcg tttttcatat ttcgtcgtgc ccagggggta ggaaccgtcg
     3541 gcatagggga cgaattcact gaccgggatg gtatcaccga gctgcaggtt agccggttcg
     3601 acgactttct tgatgaattc cggaatatcg cgggtatctt tgacgacggg gtcagacggc
     3661 aggtctttcc atgcttccgg tacagtgact tcgacgatat ccgaaatgcc gcggtcgacg
     3721 gcagcctggt tcatggcgat gacttgttcg cctttggcca gataggtctt ggcaatggcg
     3781 tctttcatgt atttcacggc atcgtcgatg ggcaggatgt tggccagttt gaagaaagct
     3841 gcctgcagga ccgtattggt gcggttgctg cccaggccga tttcttcggc gatcttggtg
     3901 gcattgatga tatagaagcg gacgtgcttg gaagcgagct ggcgttttac tttattgctg
     3961 aggtggttgg cgacgtcgtc tttatcccag ctgcagttga gcaggaagat gccgccgtcc
     4021 ttgatttcac tgacaatatc gtaagtcccc atgtatgcct gtttatggca ggccaggaag
     4081 tccgccgatt tgatatagta cgtagcccgg atcggattct tgccaaagcg caggtgcgac
     4141 ttggtgacgc cgccggattt cttggtatcg tattcaaagt aagcctggac atacatatcc
     4201 gtgttgtcgc cgatgatctt gatggagttc ttgttggcac caaccgtgcc gtccgagcca
     4261 aagccccaaa acttgcagct gatggtccct tccgtgtcga ctgtgatagg tacgtcggcc
     4321 agggaatgat gggtaacatc gtcgatgatg cccagggtga agtggtgctt cggctggagc
     4381 gaatccatgt tggcagcaga aacatgcgta caacaagctg ctctgcacag gagagcacaa
     4441 cgcgggcaga gggctgcgcc agaagcccct tcagcacaaa taacaacgcg gcagc
//
LOCUS       DAWCYS010000121         4476 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1071_length_4476_cov_7.337706, whole genome shotgun sequence.
ACCESSION   DAWCYS010000121 DAWCYS010000000
VERSION     DAWCYS010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_4476_cov_7.337706"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1136 a         1080 c         1157 g         1103 t
ORIGIN      
        1 acaatgtttt ctaatatttg gtcgatatcg cgtgcattgt ggccatatac ggcttctcgc
       61 aggccatggt caacgacata gtacttttca ttgatagaaa gcagtttctt ccctaataaa
      121 tcttggcggg ggattttata gaataaataa gctgttgtac aagcattcag gtaattcaat
      181 acggtatcgt tggaaacttt tcgatgttca cttttcaagt attttgtaat gttagaagca
      241 ctgaaaacgt ggccgatact gctgagggca tatccgatga tgcgctctaa taaatcgacg
      301 tcgcgcagat tattccgttg gacaatatcc tttaaaatta cggaattgta catatcctgc
      361 aaatactgac gagatgggcc ttcttggaaa tggagattca ttaagaaagg catcccgccg
      421 aagagtaggt atttttggaa ggcttgcggg gtatcgtcta attgatttgc taatataaat
      481 tcttgataag aaaaaggata aatgacgaat tgaacatagc ggccggctaa ataggtggaa
      541 agttccccag aaagcagctt agcatttgaa ccggtaagat aaatatcgac atcgaaagcg
      601 acacgcaggg agttgatgac ccgttcccaa ttcgtcactt cttgtatttc atctaaaaaa
      661 atgtatattt ttccttttat ggcagctgct ttgtctgcaa tccattgata gagtgccatg
      721 ggttggcaca aggggagatt ggccaaatct tcaaaattta ggcttatcat ttgatcagga
      781 cggacttgtt gagataatag ttcttcctgg attaattgca gcatgactga ttttccgctg
      841 cggcgtattc ctgtcaatac ctttatgaga tccgtgttaa tgaacggtct gatttgtttc
      901 atgtaatatt cgcgctgaat catggtatcc catcctttct gaaaaactat ttatactatt
      961 agaccagtat attttacagt tttccataat tcatagtatt atactagtat gaattatgga
     1021 aaataggaat tctaactagt atagtagttg gatttttcgt ttacttgtga ggatacgctt
     1081 cttttgctat aatgaacggg taacgtacag gttacggact tgatttttta gagataacga
     1141 ctgtgcgtcc ttttgggcgc cttcgttatc tctttttttg ttgggaggaa tcattgtgga
     1201 agttcgtcat tatcattgga aagatggaaa atccctgact gtcggggaac ggacgctggt
     1261 catgggcgtc ctcaattata cgcctgactc tttttctgat ggcgggaaat ggaataatgc
     1321 cgatgtagcc ttgaagcaca tggaagaaat ggtagccgaa ggggcggata tcatcgacat
     1381 cggcgccgaa tcgtcacggc cgggttttac gccgatttcg gcagccgaag agatttcccg
     1441 gctggaaacg attttgccgc gccttgtcaa agcctgcccg gtccccattt ctgtcgatac
     1501 gtataaggct gaaacggcag actatgccat gcgtacgggt gcccatatca tgaatgatat
     1561 ctggggcctc cagtatgcgc cggaaccggg acagatggcc gctgtagccg caaaatacgg
     1621 cgttcctgtc gtcgtcatgc ataaccagga cggtacagaa tacggcgaca tcatcgagga
     1681 tatgaagcgc ttcttcatcc gctcggccat cattgcagac caggccggca tgagccagga
     1741 ccagatcatc accgacccgg gcatcggctt tggcaagacc tttgaccaga atgtctacgt
     1801 catgaagcac ttgcgggatc tgacaggcct gccgtatccc atgctcctgg ggacgagccg
     1861 caagggcttt atcggcaaga tcctcgacct gccggttacg gaacggatgg aagggaccgg
     1921 gacgacctgt gtcgctggcg tcctggccgg ctacgcgatt gtccgcgtcc atgacgtcaa
     1981 acccatcgtc cgcatgtgcc agatggccga tgctctgcgt tagataaatt agtcaggtaa
     2041 aaccttttac tgtataaata tgacggaact tccttgactg ccgtggcctg gcggcggtat
     2101 aatggctcaa atgtatataa gctgataggg aggtttctac atggaaaata aaagtctgcg
     2161 cggcctcgtc gtatgccgca attttttaca ggatcccgtc atcaaggccc tgctggccgt
     2221 ccaggaacag ggggataatc cctttggcaa gcatgaagcg gcagcggtcc tcttggaacg
     2281 ggcggaacag ctgggcctca gcggcaatat cctgcgccag tatttcctct atctcttggg
     2341 ggaaggcgat acggtcgctg ccgaagccat tgaacggtcg ggcaaggccg gcacgggcat
     2401 gaccaaggcc ctgctcttgg atatgactct gctctggccg tatctgcaaa aggccgcttc
     2461 cgacttcctc ggcgtcgatt tcctcgacaa ctacgaaccg gctgtcccca aagtttacgg
     2521 ctatgtccag accctggaaa cggccctcat ggcagcgtcg acgccagaag aagcgacgaa
     2581 agtcttactg catcattatg cagtatacgg ccggggcaaa ctggcacagt tcatggcctt
     2641 ccgcatcggt gaagacgggg acctcgtagg catcgagaat ttcccccatc ttgcctggga
     2701 tgacctcatc ggctatgcgg ctcagaaaga aaagctcctg gccaatacga cggcctttct
     2761 ggctgaccgg gcggccaata acgtcctctt gacgggttcc cgcgggacgg gcaagtcgac
     2821 ggccgtcaaa gccctggtct gccgctacca tgcaccgcgc gtgcgcctca tcgagggcca
     2881 gggcggccag ctccacaatc tgcagcagct catggaaaaa ctcggcgaca tcggcagcaa
     2941 gaagttcatc ctcttcttgg atgatttgtc cttcgatgaa aatgaaaaag agtataaata
     3001 cctcaagtcg gccatcgacg gcggcgtcac gccacagccg aaaaacgtgc ttatttatgc
     3061 aacatcgaac cgtcgccacc tcttgaagga aacgtggcac gaccgcaacg acgaactgga
     3121 cgaagtctac cgcgatgaca gcacgaacga gtcgatttcc ctgtcggacc gcttcggcct
     3181 catcctccat tactcggcgc cgacgcagga tgaatacttg cagatcatcg cccatgagct
     3241 gaaaaaagcc ggcattaccc tggacccgga agagcttcgt gtcgaaggcg tccgctggga
     3301 aatggaacat tccggccgca atggccgcat tgctcagcag tttgtgaaat ggtatctggg
     3361 caatcgcaag gcttaagaat cttaggcaag taaacgttta accaaacttt tttcggaaaa
     3421 attccaaaaa agcattgaca aacagcacgg cattttgtag aatgtgttca agcttttcaa
     3481 acgggccaca acgataaagg ctcgcaaaag catatagtga aatctgatga cgggaaagag
     3541 tagttcgtcc ggacattccc agagaaccgg cggctggtgc gagccggtaa tgaatggcga
     3601 ttgaaaatca tccccgaaga gaagtgctga agcaagatta agcactgacg atttttccac
     3661 gttataggaa acgcgtatgt tggtacgttg tagagagcct ggctgcagta atgcgccggg
     3721 aatcagggtg gtatcgcgga atagtactcc gtccctcagt gaggggcgga gtttttttgt
     3781 tggcctcttt cgacgaatct ctaaatccca acgagaataa attattttat actcacaagg
     3841 agagatgtta ttatggcaaa agtattttat gatcaggacg taaattggga cgttatgaaa
     3901 ggcaaaaaag ttgccgtcat cggttatggc agccagggtc atgctcatgc attgaacctc
     3961 aaggacagcg gcgtcgacgt cgtcgtcggc ttgtatgaag gcagcaaatc ggttgaaaaa
     4021 gctaaagcag ccggcctcga agtcaagacc gttgctgaag ctgtcaaaga tgctgacgtc
     4081 acgatggtcc tcattccgga cgaaaaacag gctgacgtat atgcaacgga aatcgctccg
     4141 aacttgaaat ccggcagtgc cctcgccttt gcacacggct tcaacgttca cttcaaacag
     4201 atcgtcccgc cggctgatgt cgacgtattc atggtcgctc cgaaaggccc gggccatctc
     4261 gtccgtcgta cattcgtaga aggcggcggc gtccccgacg tattcgctgt cgaacaggat
     4321 gcttccggca aatgcttcga cctcgctttg gcttatgccc gcggcatcgg cggcacgcgt
     4381 gccggcgtca tccagacgac tttccaggaa gaaacagaaa cagacctctt tggtgaacag
     4441 gcagtcctct gcggcggcat ctgccagctc atcacc
//
LOCUS       DAWCYS010000122         4372 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1097_length_4372_cov_6.118138, whole genome shotgun sequence.
ACCESSION   DAWCYS010000122 DAWCYS010000000
VERSION     DAWCYS010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1097_length_4372_cov_6.118138"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1131 a         1133 c         1063 g         1045 t
ORIGIN      
        1 tctcaatcac ctatatagta gggtcttgat cctggcaagc ccgtcccaga acattagcga
       61 gtctcaagtt cagggaatgc attgccaaat ctctagcggg tcgcccacgt ggcgacccct
      121 acagtgaaaa aaagagaggc tgtcttaata cgacagtctc tctttttttc actctttcag
      181 cacataccca taggcgtgga tgcgtccatc gtcttctttt tccagataga cgccctggat
      241 ttccgaggcg aagcccggca attcattgag gccttcttcc agggccttga aatactggac
      301 tgccgtatgg gaccagcgtt cgcccgggac gacgcagagg acgcccggcg ggtacggcag
      361 agcgccttcg agggcgatgc ggccttcgat ttcatcgagg ggcaccagct caccctggcc
      421 gcggatgaaa gcgtattgcg catcctgggc attcatggcg tattccggca agtattcccg
      481 acggaagagt ttcttctgca ggtccttgac gttccgcgcc ttatagaaat catgcatttc
      541 ctgacagagt tggcgaatcg tatagccggc gtagcggtcc ggatgtgagt tgtagacgcc
      601 gaggaggact tcctgcatgg ggacatcgtg gtcgacgaag cgttcaaaac ggaccagctg
      661 ggacacgagg aggcgcatct tattcttatt ttcagccggt gtcagcagga agaggatcga
      721 attcaaatca catttttccg ggatgacgcc gttaccgcga aggtacgtcg ccaggatgtt
      781 ggcatggata ccaaaatctt cgtactgtcc cgattcagcg tcgatgcccg gtgtcgtcaa
      841 taacaatttg caggggtcga cgagatattg tcccggcccg tatccttcaa aagcatgcca
      901 cttggcatcc ggttcaaagg taaaataggc caggtcgttg gccatgtcat ccgtattgcc
      961 atcctgccag ggcttgccgt agaccgtcgg tgccacgaac ggccggatat agtggcagcg
     1021 gcgcaggata tccttgcggg cttcgatacc cattttcacg cagtccatcc acatcttgcg
     1081 gccagccgca ccggcatgga ttttggcgtt gacagcgata gtggcgaaca tgggataaaa
     1141 aggactcgtc gacgcattgg ccatgaaggc attgttgaag gtcttgtggt tgcagtagcg
     1201 gggctggtta tggatatggt tgtccttctt atggatctgc gaagcctgag aaaaaccagc
     1261 ctgctgctta tggaccgact ggacgaccat gatacccgga tcgtcttcat gcaaatcgag
     1321 gagcagcggc gagtagtcct tgagcatggg aatgaactgt tcatagccga cccaggccga
     1381 atcaaagagg atgtagtcgc agagatgacc gatgcggtcg acgacatagc gggcattgta
     1441 cagcgtcccg tcataagtgc cgagctggat gacagccaag cggaaaggcc gcttggcctt
     1501 ggctttttcc ggatcccgtt cggctgccag ctggcgcaga tacgtttcat cgaaacagtg
     1561 ggcgtcgatg ccgccgatga agccataggg attgcgggcc gtttccaaat agaccggcgt
     1621 cgccccggcc tggatgagag ccccgagagc gacggacttg tgattgttgc ggtcatagag
     1681 aaccaagtcg ccaggcgtca gcaaggcatt ggtgacgacc ttattggacg acgaagagcc
     1741 attgaggacg aaataggtct tatcggcatg gaagacttcg gctgcaaaat cctgcgcttc
     1801 caaagccggc ccttcgtgga tgagcaaatc accgagggtc acgtcggcat tacagatatc
     1861 ggattgaaaa atatgaggtc catagaaatc ataaagatag cgtcctgccg gatgcttctt
     1921 gaaaaacagg ccgtcctgat ggcccgggca gtcaaaaggc gtattctttt cactgacgta
     1981 atcgacgagg tcggcaaaaa acggcggcag cacagcatgt tcatagcggc tggccaggcg
     2041 ttcggccgtc gaaatacagc ggctcttatt gaccatttct tccgtaaaga cgacatcgac
     2101 cttgtcgata tactggtccg gcatcttctc gccgcccggc atgatgacca tgatgggaat
     2161 accgaatccc gtttcatcga tcttgtcgat gtaggcaaaa tcctggtccg aaataaccgc
     2221 tacggccaca tcggtgaaat ccgtctgctc aacaggcacg atttcccggt cgctgtcaac
     2281 gaaataatac tgtacatcaa agctgtaagc tatcttcaaa tgtttcatat gtcttctctc
     2341 cctatcctct tcctttgcat tgatatttta ataaatacag ccctattgta cgcaattccc
     2401 ccatgaatag caagaaaaat atttcgcaaa tagtacattg gggcctaaaa agccgttcat
     2461 cgtaggtcgt tcgtcgttca tcgaatatgg ggcctgacgg ccccgccggt cgcaggccgc
     2521 ctcggactca actgccgcta cgcggcaaca gacccagaat gacgacatcg cctcgagtgc
     2581 cccttggctc gtcaaaaaat agtggctcgt tgttcgtggc tcgtaaaaac ttaccattat
     2641 actcggatcg gctgtgacca aatatcgcag cgggccgcac aaggctccct ttatagggga
     2701 gctggccgcc aaaggcggtc tgagaggtta aaggtcgccc cctacgcaca aggccgttgg
     2761 ccgtgtattt ttttgcgaac tgcgacctga gctctatact ggtcatatta tcaattgccg
     2821 agtataataa aagtgaaacc tcaaaattta cattttttga ggtctcagaa tcatgagata
     2881 gcaaaaatcg ctttttttga ggtaactgcc atgaaatatg aatccctgta ttcactctat
     2941 tacaaagaca gccaaatatg ggaaaaagta tatcaggaac ggtttcacag cccctttagc
     3001 cgtcatttgc ctttggcgat aaagcagtat catcgtcggc aaagccatcc tgcttttttc
     3061 tgctatagcg aagaaatcgc cttatccttg gaaaaaattg ccagcgaagt catggactgt
     3121 cttgacatta tacatcaagt tccaccggcc gctatctcgc agtttttgca tacgtcccta
     3181 gttgatgaaa tcaaatcgac caatgacatc gaaggcgtgc gcagtacgag gaaagaaatc
     3241 ttgacggctt tttccgtcca cgaatcggaa cagtcatttt accgtctggg cagcatcggc
     3301 aacaaataca tcaaaatcat tcaaaaaaaa tacatccctt taactaccag ccaggatatc
     3361 cgccagcttt tcgacgattt tctggccgat gaaattaaac gagatgaccc caaaaatctc
     3421 cctgatggga aaatctttcg taaggatcgt gtcgacatcg tctcaccggc acaaaaaact
     3481 atccatcaag gagtctatcc ggaatcagct atcattcaaa tgatggacgc agccctcgat
     3541 gtgctgaatg acaagaccat cccttattta atccgtgttg ctatctttca ctatttcttt
     3601 ggctacatcc atcctttcta tgatggaaat ggccgaatgg cgcgattcat cacgtcttat
     3661 ttgttggcat tgcatttaca tcccgcaata gccctgcaag tttctatctt aatcaaaaaa
     3721 taccgcaaaa gatactacga cctgttttca cataccgatg ccgacatcaa tcgcggagat
     3781 ttgaccccct tcatcatagg aaccttgaaa ttcatcgaac aggccacgct tttcacacac
     3841 catacactca aacataaata tcaaacttat caaaaagcca aagaaaaact cacccaaacg
     3901 ccgtccttgt caacgaaaaa taaaaccacc gctgcgattt gcgatatcct gctacaagcg
     3961 gctatctttt ccgacatcgg cgtttccgta aaagaaatca tagaaaccct aaacatttcc
     4021 gaaaacacag tctacaatca tctgaaaaaa attcctgaag aatatctgtg gataaataaa
     4081 gaaatacggc cataccgcta ccaattgcgg ttataagggc ctgacggccc cgccggtcgc
     4141 agttggcagt tcgcatgccg cctcggactc aaatgccgct ttgcggcaac aaactcagaa
     4201 tgacgacatc gcctcgagtg accctcggct cgtgaaaaaa tagtggctcg tggctcgttg
     4261 ttcgtggctc gtaaaaactt accattatac tcggatcggc tgtgaccaaa tatcgcagcg
     4321 ggccgcacaa ggctcccttt ataggggagc tggccgccaa aggcggtctg ag
//
LOCUS       DAWCYS010000123         4368 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1099_length_4368_cov_6.040575, whole genome shotgun sequence.
ACCESSION   DAWCYS010000123 DAWCYS010000000
VERSION     DAWCYS010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_4368_cov_6.040575"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          870 a         1212 c         1301 g          985 t
ORIGIN      
        1 aagtgtaaag gatgtccttg cacaaaatgt aaacgatgtt attcttgaca acgtgcgagc
       61 ccctacaaac gtcttacagc gagcacctac aaatgccctc caggcacctc tcttatcagg
      121 aaaggccagt tacgaaccac tagccacgaa taatctcgct ccaggcgttg gcgatgtcgg
      181 cgaattcctg gagagacagt gtttccccgc ggcgtttgcc gtcgatgcct gttttttcca
      241 ggacctgggc gatttgttcc ttgctcatgc ccgtgttttt caaggcgttg gaaaaggtct
      301 tgcggcgctg ctggaaggcc gctttgacga cctggaagaa gcgcttttcg tcgattaaat
      361 cgacaggcgg ctgctggcgg acgtccatgg cgacgacggc cgacgtgact tccggtgccg
      421 gcatgaagca tttcggcgag atttcaaaga gcatctgcgg ctgcgtatag tactggaccg
      481 acaccgacag agccccgtaa atcttatggc ccggttcggc taccatgcgt tcggccactt
      541 ccttctggac catgacgacg atgcgcttga tgggtagctt ctgttccagg agcttcatga
      601 tgatcggcgt cgtgatatag tagggcagat tggcacagac cgtgaagggc tggtgattca
      661 tgacttcttc aatgtcgatg cgcaggatgt cgccgtggac gacgcgcacg ttgtcatagt
      721 gttccaaggt cttgtcgagg acgggcaaca gatggtcgtc cagttcgaca gccgtgacgg
      781 ctgcgcccgt ttcggccagg gcctgggtca gggtgccgat gcccggcccg atttccagga
      841 ccggatcgcc ctccccgatt tcggcagcgt cggcaatgtc gtcgacgacg gcgtgacgga
      901 tgagaaaatt ctggcccagt ttcttattca tgcgcaggcc aaagcgtttg acgacgtagc
      961 ggacgacgtc gggattggac aagtcgactt ctttcattta tttcacctcg tggatctgac
     1021 ggagggcatc atcccattcg tcacgggtga cgccgtaatg gttcaagcgt ttcaaaaact
     1081 gtttggcatt gccatagccg atacccagga tggcgccgac ttcggcccgg cggtcggccg
     1141 catcaggcgt cccgttgagg ccagcagcgg aaatgtcctg catggtaaag gtcgcctgcg
     1201 gttcatacgt cgcacagcgg actttggaca aggccaggcg gatggcttcc ggcgaggcct
     1261 gttcgacgcc caggtcgttg ttggcaatgg catctttcct ggggataaag gcgtgtttgg
     1321 cattagggaa gcggtcggcc aggaacttgc ggatctgttc gccggcccgg tcggggtcgg
     1381 tcaggatgat gatgccccgc ttttcataag cacagcggat ctgttccagc gtgtgaggct
     1441 tcaaattgaa accatctgtc gtaatcatat cgacgtcgat gtgactggcc cggatgcggg
     1501 cgacatcgga ccggccttcg acgacgagga cttccttaat catgagcggc tccttcccgg
     1561 tgttcggcaa tgtactgggc tgcgctgagg atggccagga cattgtgttc aaagacgatg
     1621 ccgccttgga gataaatcat gtacgggtcg cggatcgggc cgtcggccga caattcgata
     1681 gacgaaccct gaacgaaatc accggcggcc atgatgatct tatcggcata gcccggcata
     1741 tcgtcgggaa tgggtgtgac gtgggcgtcg acaggcgaga aggactggat accctggcag
     1801 aagagacaca tgttctgttc gttttccagg taaatggtct ggatcaggtc gacgcgcggg
     1861 tcctggacgg ccggcgagac cttgaagccc atcttttcaa agacagccgc tgcaaagacg
     1921 gcccctttga tagcctgagc cgtaacgtgc ggtgccatga agaagccctg gaaaaagagg
     1981 cggtagccgg ctgcatagga accgagttcg tcgcccaggc ccggagccgt caggtagtcg
     2041 gctgcggcgt ggaccagttc ttccttgccg gcaatatagc cgcctgtggg agcaatgccg
     2101 ccgccgggat tctttatgag cgacccggcg atgatgtcgg caccggcttc aagcggttct
     2161 tcttcagcag caaattcgcc ataacagttg tcgacgaagc aaatcgtctt ggggctgacc
     2221 ttcttgacga cggaaataat agaacggata tcatcaatcg tcaaggatgc ccgcgtgctg
     2281 taaccgcggg aacgctggat gaggaccagt ttcggctgct tttcggcgac ggccgcttca
     2341 atgccggcca aatcataggc gttgtccttc aaggggactt cctcatagcg gacgcccttt
     2401 tccttcaaag agtgcggcgt ttcatgggca tagccgatga cgccctgcat cgtgtcataa
     2461 ggcgccccga cgaggctcat catgagatcg ccggtctgca acagggccag caggaccgtc
     2521 gccagcgcat gggtccccga tacgaagtgg ggccggacaa tggccttttc ggccttgaag
     2581 acgcgggcaa agacttcatc gagcttttcc cggcccatgt cgctgtagcg atagcccgac
     2641 gtaccggcaa actggtacta agaaatctgg gcttccttat aagcggccag gactttcgcc
     2701 gtattatgga gacaaatcgt atcaatgcgt tcaaactggt ccgcacattc ggccagcgct
     2761 tcttttcgta atgtttctaa atccaaagac aatgtcatga ttcctttcta taatgaatgt
     2821 ataagttagt ggctagtggt tagtgaatag tagttagggg ttaggggcta gtggctctcc
     2881 tcataggaga gctgtcagct ctgctgactg agaggttctt ccagacaatg agacgctata
     2941 ccgttcagga ccttttttca tttcgggccg ccctccggga cggcccctac ggagattgct
     3001 tttttcctta cagtccgctg tttcgcaggt cgcaggtcgc tgaaggaaac ctctccgcct
     3061 tacggcacct cccctatcag gggaggcttt cggctctcct cataggagag ctgtcagctg
     3121 cgggccgccc tctgggacgg cccctacgga ggttgctttt ctacttgccg cctgcgcctt
     3181 cccgtcatgg gaaaggcaca tcccccagca cagcctgtat caatgcttct tctccatcgt
     3241 actggtcctt gtcgtaccag tggatatagg gcatgcgttt gaaccacgtg agctggcgtt
     3301 tggcaaagcg ccgcgtccgg gtcttgatgc gggctgcggc gtcatcgagg ggcatctgtc
     3361 cctggagata cagggccagt tcctgatagc cgatggcctt catggactga cagcccggcg
     3421 ggatgccctt gtccagcagg taccgcactt catcaaccca gccggcggca atcatggcgt
     3481 cgacgcgctc ttcaatgcgc cggtacaaga catcccgcgg caggcgcaga ccgatgacgt
     3541 aagcgtcata gactacatcc cgagccttgc cggcccggac gaaagatttt ccttcgccct
     3601 gctcgatggc gctgaccagg cgggtcaggc ggtgcgtgtt ggaaaacagc tcgtgccagt
     3661 ccggcggctg ccagtccgtt tctgtctcga tataggcctt cagttcctct tgactgagag
     3721 cgtcgatccg cgcctgggcc gcttccttgt cggcttcggt catgcgggcc gtctggaaat
     3781 catagccttc caggagggac tggacgtata ggcccgtacc gccgacgaga atgggtatct
     3841 tcccctttcc gttgacttcg gcaatggcct ttcccgccat atcacagaag cgggctgccg
     3901 aatagggttc gtcgggctca gcaatatcga tgagataatg gtgatagcgg gccagttcgt
     3961 ccgccgtcgg cttggccgta ccgatatcca tatggcggta aatctggtag gcgtcgccgg
     4021 aaatgaggtc ggtcccgtag tgttcggcca gggcaaaact gagggccgtc ttgccgaccg
     4081 ccgtcggacc gatgatggca attacttttt ccatgaatca cactccaata cggcatagcc
     4141 aatgcggctg tatttgccgc catataaggc aacatccggg aaatcgcggc agagctggcg
     4201 gaaatcccgt tctttgatga ccacccggcg ccgggctttc tgcagcgccc gttcgatgag
     4261 gtcccgcgtc agaccgccgt gttccatgat ggcccggacg ggctggaact ggcagctcgc
     4321 ctcgacaggc cgctggaaca tggggtcgaa atagaggacg tcgtacac
//
LOCUS       DAWCYS010000124         4347 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1105_length_4347_cov_7.283318, whole genome shotgun sequence.
ACCESSION   DAWCYS010000124 DAWCYS010000000
VERSION     DAWCYS010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1105_length_4347_cov_7.283318"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1065 a         1236 c         1143 g          903 t
ORIGIN      
        1 ctcctcggcg ctgctggcgc tgtcgaagcc attgcctgcg acatgaccat agaaacgggc
       61 aaggtccatc cgtagataaa cctcgtcgaa ccggaagatg aattgaaaga actgggcctc
      121 aattatgtac ccaataaagc cgtagaagct gacgtcaacg tcgccatttc caactccttc
      181 ggtttcggcg gccataatgc aaccctgctc ttcaagaagt acgctgaata ataaagaaga
      241 ctgacctgat ggataccgaa cgaaaacaac agttagaaca attcatacag gtcttgggcc
      301 tcaaggaatt ccacaatctc ctcattttgg atcaggccct gacccattca tcctatgcca
      361 atgaaaacaa ggccagccac ggctattaca acgaacgcct ggaatttttg ggcgatgcca
      421 tcctggacct ggtcgtaggt gaatatctat tccttcatta tcccaacatg cccgaagggg
      481 aactgtccaa ggcccgggcc agtgtcgtca gcgaaacgcc gctggcttcg gtctgtgcca
      541 agttccacat gggggactat atcctcctgg gcaaggggga actggctacg ggaggacgca
      601 cgcggccgag catcatggcc gacgcctttg aagccgtcgt cggtgcgatt tatattgaca
      661 gttcctatga agaagcccgt aaattcatcc ttcaccagct caagggctac ttggacctgg
      721 tcaagagcgg ccattatgaa aaagactata agaccctctt ccaggaatac gtccagcaaa
      781 agggcccgca gcacatcgaa tacgaactgt gccgcagcga aggaccggac cacgacaaga
      841 ccttctacat agaagtcaaa gtcaacggca aagtcctggg cgaaggatcg ggaaaaacaa
      901 aaaaagacgc cgaacaacat gcagcccata cagcgctcat ggatttgaaa ggcgaagagt
      961 tttgagtggc tagtgattag tggctagtgg ctagtattac gaaccacgaa caacgaacca
     1021 ctaaaaaaga ggctgtcgat gagacagcct ctttttttca caggaggcat catgcaaacg
     1081 tctatcatcc ctattttcat tccccatatc ggctgtcccc accgctgtgt cttctgcaat
     1141 cagtggcaga ttacgggaca ccagggcttg ccgtcgcccg atgaggtgcg ccgtctcatt
     1201 gcgtcctata cggcgtcggc ccggacgccg cgctactggg aagcagcttt ctacggcggc
     1261 agtttcacgg ccatgcccgg ggaggtacag gaaggccttc tgcagccggc ttacgaagcc
     1321 ttggaggccg gtacgattga cgccatccgc tgttcgaccc ggccggactg cattacgccg
     1381 gagatattgc agcgcctgca gcagtacggc atgacctgcg tcgaactggg cgtccagtcc
     1441 atggacgata ccgttctcga gcaggcccgg cggggccata cggcagggga tgtcgtggcg
     1501 gcgacagccc tcttacggca gtacggcttc accgtcggcc atcagctcat gcccggcctg
     1561 cccggcgaag actggccgag cctggtccgg acgacagcgg ctatctgccg gctccagccg
     1621 gacctggccc gtatctatcc cgttgccgtc ctcgccggga cggacctggc tgccagctgg
     1681 cgggccggcc gctaccagcc cttgaccatc catgaaggcg tcgtccgggc ggcctacatg
     1741 aagcagcact tcctggaaca gggcataaat tgcatccgga cgggcctgca agccacggaa
     1801 aacctggatg acgacacgca ggtcctggcc ggggcctaca cgccggctat gggcgaatta
     1861 gtcgatactc gccgctatgg tcggcagctc ctgtccttcc tccgcagcct gcccgatgaa
     1921 ccggcaatca ctatcggcta taaccggcgc gatacgtccc gcgtccgggg ctatcgcaac
     1981 gttaccgtca agacggcctg ctggagcctg cctcagcacc tgacctggca tcaggatgag
     2041 gtaccccttg gtcatatccg tgtctgccag ggccggactg tgtatgacct cgatgtgaat
     2101 agcaaaaccg ggcctactat gatataataa aatagtacgt atgattttcg catccaacct
     2161 ttgaagataa atacaacatt ggaggaaata gaatggcaag agtaaaaatc acagaaacgg
     2221 tccttcgcga cggccaccag tccattgcgg ccacgcgtat gcgcctgcgc cagatgctgc
     2281 cggtcctgga agcgatggat gaagtcggct ataatgccct ggaatgttgg ggtggcgcta
     2341 cctttgatac gtgtatgcgc ttcctcgacg aagacccgtg ggaacgcctg cggaccatca
     2401 agaagcacgt caagaagacg cccttgcaga tgttgttccg cggccagaat atcttaggct
     2461 accgccatta tgccgacgac gtcgtctacg aattcgtcaa ccgcgccgtc gataacggta
     2521 tcgacatcat ccgcgtcttc gacgccctca acgacccgcg caacctggaa tcgtccatca
     2581 aggcggcgaa agacacgaag tccgtccacg tccagggcgc cctggtctat accatcagcc
     2641 ctatccatac gatggaatcg ttcaccaagg tcgccaagga actgcaggac atgggcgtcg
     2701 actccatctg catcaaggat atgtccggcc tgctgtcgcc gtatgacgca tacaacctga
     2761 tccgcatgct caagaagcac ttgtccgtgc ctattgaact gcacacgcac tgtacgtgcg
     2821 gcctcggtga aatgacctat atgaaggcca tcgaagccgg tgtcgacatc atcgacacgg
     2881 ccttgtcgcc attcggcgaa atcaccagcc agccggcgac ggaatcgatg gtcatggctc
     2941 tgcagcacag catctatgat acggacatcg acgtcgaccg cctgtggccc atcgtcgacc
     3001 acttcaagca tgtccgcaaa gaactggaag acgaattcaa attgaccatg cccagccaga
     3061 tcaatccggc tgtccgcaaa tatcagattc ctggcggcat gctgaccaac ctctataacc
     3121 agctaaaagc ccagggcgcc gaagaccgct tcgacgaagt cctggccgaa atgcccaacg
     3181 tccgccgcga cctcggctat ccgccgctgg ttacgccgac cagccagatc accggctcgg
     3241 cagcagccct caacgtcctc tatggccgct ataagatcgt caccaacgaa gtccgcgaca
     3301 tcgtccgcgg gaaatacggc cgcgtccccg gcaagatcga tgaaaagttc cgcaaaatgt
     3361 gcatcggcga cgaaccggtc atcacccacc gcccggccga cgacttggaa ccggaactgg
     3421 aaaaatcgaa gaaagccctg gctgacgaag gattccccga tgccagcccg gaagacgttt
     3481 tgagctatac cctgttccca gaagtagccc tgccgttttt ccgtaaaaga gataacaaat
     3541 agactattga caagtcctgt cgaatcagtt aaaatagaaa agtcgacggg acgtggcgca
     3601 gcttggtagc gcgcttcctt ggggtggaag aggtcgcgag ttcaaatctc gccgttccga
     3661 ccaagacaaa agaagggttt gtcgttatgc gacaaaccct ttttgttgtt agtagctagc
     3721 agttagttgt tagcggatgg ctttaaattt aagggctttt tctgcaaacg acaaactctg
     3781 tgctacaaac tgtagagtaa aagggactgc gcatgatgac ataatcttcg tgcgacagcc
     3841 ccttttttgt cattgtaggg gctcgcacgt ggggagcccg ttccggaatt tggatgattc
     3901 ttctgacaga ctagttggga aaaatcacac atatcctgaa gcgggccgcc ctttgggacg
     3961 gccactacga aatgctaacc actagccact aaccacttct cacggttgct ttttcggcca
     4021 tttctgatag aatgatgata aatagtatag tatatatccg tttgtcatcc gcttattttc
     4081 tgtcagagga ggttttcatc atgggttaca ttttaaagaa agaccagcag gacctggtca
     4141 atttggctaa agattttgct gaaaagaaag tcgctccttt tgttcatgaa ttggatcagg
     4201 ctaaggaacc gggccagaag ttactggaca tctatcagga agccgtcgac atgggcttct
     4261 gcggcctgga aatcccggaa gaatacggcg gcctgggcca ggattattat acctttgccg
     4321 atgacaatga agctccggtc tcgtctg
//
LOCUS       DAWCYS010000125         4339 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1106_length_4339_cov_9.535948, whole genome shotgun sequence.
ACCESSION   DAWCYS010000125 DAWCYS010000000
VERSION     DAWCYS010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_4339_cov_9.535948"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          942 a         1234 c         1157 g         1006 t
ORIGIN      
        1 gtacctgccg ctgccggtcc tcttcgactc gaaagaggtc aaggaaatgg tctaccgcga
       61 ccgccacatc aagcgcgtcc tggaactggg ccgcaacgcc aacatcgcca tattcagcgt
      121 cggcaccgtc cgcgacaatg ccttgttctt ccgtctgggc tatgccgacg cccaggaaaa
      181 ggcctacctc aagagccacg ccgtcggcga tatctgctcc cgcttcttcg ataaagacgg
      241 aaatatctcc agccaggagc tgaacgaccg caccgtcggt atcgccctcg atgacctgcg
      301 caacaaagaa tacagcatcc tcctgtccgg cggcgaaggc aaaatcgcca gcatccgggc
      361 ggccctaaaa ggaggctacg ccaacgtcct catcaccgac cagttcacag ccaaacgatt
      421 actggagaag gtgtaaaaaa aggtcctgtc acatagtgac aggacctttt taagttttaa
      481 gtttgtagtt tttagtttgc agaaatatgg ctaaaaattt aaagtcaact tctacaaact
      541 acaaactccg tactacaaac tataaagaga acggggcctc gcatgatggc agaccccttc
      601 atgtgcccgc ccttcactcc caggcgctat cgggcagggc ggtgatgctg gcccaggcgt
      661 tattgatggc agcagccatc atttttttgc ccggcgggtc gagatggagt ccgtccaggg
      721 ccaattcggt cttgagttcg ccgcggctgt cggccatgcc cggcgtgatg tcgatatgga
      781 cctgcgtgtc gatgtagtcg ttgacttcgg caatggcgcc gcgccagtca tcggccgtat
      841 cctggtcgaa ggcagccttg atgttctccg gattgagcgg cggaatggtc aggaaaacgg
      901 gcttgatgcc atggctcagg catttttcct tcaacgtctg taagtcactg atgacttcac
      961 cggccggcac gccggcgcgg aggctgttgg agccgatgag gataatgaca tacgacggct
     1021 tgaagggcag gacgtcgcgg tcgaagcgtt cgacgcccat ggcgctcgta tcgccgctct
     1081 gggccaggtt gatcgtcggg aacatgagat acgaagcgta actgaagtcc caatccgtcg
     1141 gcgaatagga aatactgccg ccaccgtggc tgatactgtc gccaaagata gcgacgacgc
     1201 cctgttccgc tgccgggtcc agctcaaagg gcagggcatc ggaataaccg ccgataggat
     1261 tgcctgcccc atccagagcc aggacgcgcc agtaaaaggg cttatcgccc atgcgggcct
     1321 tctggtcgta ctgctgggca tattgcggcg tgtaactgtc gatgcgatga atcgacggcg
     1381 ccgtaccatt cggatcttcc ggcaaatcgt tgaggatttc tacttcatac tttgctgccc
     1441 ccggtacggc tatccagtcg tagaccggat agagcagggt ctgtcccggt ccctggttga
     1501 aaaaggacaa gggcgtcggc ttttccgtaa aaggctggaa aatatcgaca tgcgcttctt
     1561 cgatatccga aaattcactg accggacggc ccttcaaatc gaggccgcgg acgcgccagt
     1621 aaaagacctg tcccaggaag gtatcgggca aatccagttc cagtcccgtc gtataagcct
     1681 tctggtcatc catgaaaggc tcgtaagacg tactgacctt tccgtcatcg cctttttctt
     1741 cctctttctt caagatctgc acgtcataca tgacagcccc gtcgacacgg ctccagcgca
     1801 ggatgggatg gccatcagcc ggatccgtcg ccggataatg gagagtgaca tgcggttttt
     1861 cccgcaaagc cggcatgtca tcactatcgg cctttacatg ctgccgcgct tcttccaggg
     1921 ccacgtcgac accgtgggag tccgtcgact gacgggcgcc ggcaaagatg ccgaccccca
     1981 gggtcagggc catgagagcc gtgataaaag ctaattgttt acgcattctt ctccttctta
     2041 tctttcttat aaccgtactt ctgggtccag gctttattct gcttctggat ttccttcttg
     2101 ctcaacatgg ccggcttggc ttccagggcc gactggatat tcttgacgat agccggctga
     2161 tcatcaccgt cagcacaggt catgatgcgc aagtcgttga catagacatc gttaccgcgg
     2221 gacgctacgt tccacgacgg ctgcttcttg ttcatcgctt ccgtaacgac cttgtcgtct
     2281 ttcgtcgctt cgccgtagac gtcatgcgta gcccgggcca gattgcccgc taccaggaaa
     2341 gcccggcgcc gcgacgaata cgtcccggct gcagccggtg tgacgacggg tttggcattg
     2401 acatagaccg tgttgccgcc gacctggacg cggttgcccg accacaaggt catgcggccg
     2461 gccagctttt caatacggct cgacatctga ggatgattat tcggactgat gacgcgcttg
     2521 aagccttcct gccacagttc gccgtactgt tcgtaaacgt actgcatgga cgaagccgcg
     2581 ccgccgacat tgtaggccgt gttcttgaag acattgaagc ccgtcgcatc ggcctgtttt
     2641 tcctggtcca tagtgacgaa ttcattgtcg atgtaattga cggccacact gcccaggaga
     2701 atgtcgagga cgtcggcatt accgccaagg gaaacgtcga tgagcgtcga aataccgata
     2761 ctcttgagga cgccgttgat ggcatggcgt ttttcgccgt gctgcatttc atggcacatg
     2821 gtaaaggcca gttcatcgtc gttgagggca tcgagcatcc ccttattgac ggaaatgacg
     2881 ccgccaatcg tttcaaaggc gttcaactct ttcgacgggt tggcatagac atcgtaattg
     2941 cgcttgatga ggcccgtcgc tacgaggttg cgctggatat tttccagacg gtcgctgtag
     3001 gcttcattat cgacgacacc agtcttggcc tgcgtattgg ccaacatctg cttctgattg
     3061 ttgttgtcca tctggagcag ctgctgtttg gccagagcca tagtcgctgc cccatagagc
     3121 aggatctgcg ccgtcgacgc agccgaagcg atggcgacag gcgccacgca gagagacagg
     3181 ctggcaacga gagtcgtcag ccattttttc cataatttca tccctatcat cctccctcat
     3241 ccgttagcga aagctgttcc cggcagagct tctccttctt ctttttcgtc cactgcttga
     3301 tttgccattc ccaatgcatg gcctcgtgtt ttgtaggaaa accctggcac caggccaggg
     3361 ttacagggcg acgggaacgg gtatacttag cccctctgcc gtcattatgc gctgcgaccc
     3421 gccggggcac atccagcgtc cagcccgtat ataaagactg gtcggcgcag cgcaccatat
     3481 acgtataata taacatgaag cccttaggct tcttccgtga cgattttcac cgtattcagg
     3541 acaacatctt ccagcggttt atcggcaaaa tcggtcttga ctctgctgat gccttcgacg
     3601 acgtcataat ccttgacgac cttgccgaaa atagaatgat ggttctggag ccacggcgtc
     3661 gggacgaccg tcacgaagaa ctgggaactg cccgtattag ggccggcatt ggccatagcc
     3721 aggacaccgg gttcgctgaa atcgaggccc ggaccgaatt catcaggaat cgtctgtttc
     3781 gagccgccgc atcccgtacc cgtcgggtcg ccgccttgga tcatgaaatc ggcaatgatg
     3841 cgatggaaaa tcgtgccatc ataaaaaccg gattttgcca aattcacaaa attttcgacc
     3901 gtaatcggtg ccttgtctgc aaaaagttct accgtaaaat cgccgcggtt cgtctggaac
     3961 acagcgtatg ttttattgtc tgccatagta acagcctcct gtgtatataa taaggtattg
     4021 tatacgttta tattaccgta gaataggcaa ttccgcaagt agttagggcg ctaaagcgcc
     4081 cgcaggccgc ctcggactca gatgccgcta cgcggcaaca gactcagaat aacgacatcg
     4141 cctcgagtaa ccctcggctc gtaaaaatcg ccccaatcgg ggccttttcg taggggccgt
     4201 cccgacgggc ggcccgccgc gaaattccac cacagctcac tctggcatga tggcaggttt
     4261 tttgaacctc tcccgcttat agacgcggga gattcttgag aaggttgctg tttaagtttc
     4321 ctcctgaaat caggatagc
//
LOCUS       DAWCYS010000126         4336 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1108_length_4336_cov_4.669703, whole genome shotgun sequence.
ACCESSION   DAWCYS010000126 DAWCYS010000000
VERSION     DAWCYS010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_4336_cov_4.669703"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          886 a         1149 c         1225 g         1076 t
ORIGIN      
        1 agcctgccgc caccacggcg tcgccagcca gggctgggca aagtttgtgt aaaatatcag
       61 ggaaagcctt gacaagtcct gtcttaatgg attatattag aatttaggtt tgaaggccct
      121 cattctgcag aaagggagag taccgtaatg attcaagttt ataagcacag caacggccat
      181 ctggaagaca acatttccct ggcatcggct gaaaaaggat cctggatcca cgtcgtaaat
      241 cccgattccg aagatttaca gctcgtatcc atggtcacgg aaatccccac tgatgttctg
      301 aaaaccgcct tggacacgga agaacgttcc cacgtcgaaa tcgaagataa ctacatcttt
      361 gtcgtcatca acatcccgat catcttggaa acggacagtt atgacacctt gcccctgggc
      421 attttcatta caccggactt catcatcacc ctctgtattc aagaaacaga agtcatgcgc
      481 gcctttacgg agaataagta tcctctgttc tatacattca agaagacgcg tttcttattc
      541 cagatcctgt accgttcggc gacgctgttc ctgcgctact tgatgcagat caaccgccgg
      601 accgatgaca tcgaggaaat cctgcgccat tccatgcgca acaaggaatt cttcctgctc
      661 ctggaattac agaaatcctt gacgtacttc acgtcggccc tgcgcagcaa tggcatcgtc
      721 atggaactgc tcatgcgcct gcgccgcaat acgtcgctcc agctcctcat caagatgttt
      781 tcagaagacg aagacctcct ggaagatgtc atcatcgaaa acaagcaggc tatcgaaatg
      841 gtcgagatgt attccaacat cctcatgaat atgtccgata cctttgcatc catcatttcc
      901 aacaacctga acttggtcat gaaattcctg gcttccatta caatcatgct ggccgtcccg
      961 acgatcatct tcagcctgtg gggcatcaac gtccccgtcc ccttccagga cacgacagcc
     1021 ggcttctatt acgtcgtagg catcgccatg ttctgcaccg tcgtcgccgt catcatgctc
     1081 tggcggaagg atttattctg aaaggccctt caccatgctc tttatcggtt caaaagagcg
     1141 gcggtgaatg ggacagggcc cgtatttttg caacgcttcc atatgcattt tcgtcaaata
     1201 tcccttgtgc acggcaaagc cgtactgggg atattttttg tcgtattcga ccatgaggcg
     1261 gtcacgcgtg actttggcga tgatcgaagc ggcggcgatg gacgcgctca gggcgtcgcc
     1321 gtggaccagg gacttatggg gcacggtcag ttcccgaaga ggcatggcat cgaccaggac
     1381 ggcttcagcc ggcacggcca ggccgttcac ggcgtcgtac atgccctgca tggtggcgtg
     1441 gtaaatatcg agttcgtcga tttctttttc cgacttggcc acacaggata cggcaacggc
     1501 ttcggccata attttatcat agaggacgtc ccgcttggcc ggcgggactt ttttgctgtc
     1561 attgaggccg ggacattccc agtagggcgg caggatgacg gcggctacca tgaccggacc
     1621 ggcagccggg ccgcggccgg cttcgtcgat gccggctacg tggtacagcc cttggttgta
     1681 gtaagccgtt tcctgggcat acatgtgcag gacccgttcc cgttcggctt cttcccggat
     1741 tttctggcgc atataggcgg cagccagctt gcggaccgac acgcggccgt cttctttcat
     1801 ggctgccagg aggtcctgga cgtcaccgcc agcggccagg cgggccttga ggtcggcgac
     1861 gcggagtttc tcgatgtcca tggtcagtcc tccccttccc attcgcccag ccggtccagg
     1921 gagatggcgc cgatcttgcc ttcgcggaat tccttgagga tgagcttgta cgtcttttcc
     1981 agggcgacga ctccgccgga aacgatacag ccccgcttgc ggccgatggc ttcgatcgta
     2041 tccagcggct tgtcacagag ggtttccagt ttataacggg ccttcaattc atcagggtac
     2101 tgggccgata actgttcgat gagctgcatg atgaccgttt ccatgtcgaa gaccgtatcg
     2161 gaaatggccc ctgtcgctgc caggcggtag gcgctgacct ggttttccag tttcggccac
     2221 aagacgcccg gcgtatccag gaggtcgagc tggtcgccga tgtggaccca ctgcttgccc
     2281 ttcgtttcgc caggcttgtc ggccgtcttg gccgcactgc ggcgggacag cttgttgatg
     2341 agcgtcgatt tgccgacatt aggaatgccc aggatcatgg tgcggacggc gcggttcttg
     2401 aggccccggg ccttccagcg ggccagcttc ggcgctgcca gggagcggac cagggaaatg
     2461 agctgtttca cccctttgcc gcctttgctg ttcagggcca ggactttgcg gccctgctgc
     2521 ttgtaatagg ccacccattc gtcgaggacg gccgggtcgg ccaggtcggc cttgttgagg
     2581 acgacgacag acggcttgtc gccgatgagg gacttgatca tgggattggt gctgctcata
     2641 gggatacggg catcgacgag ttcgatgacg acgtcgatga tcttgagatt ttcctgaata
     2701 aggcgcttgg ccttggccat gtggccggga taccagttga ttatcatagt gaatctcctt
     2761 tggtgatgag aaaaaagggc ttgtcgaacg acgacaagcc ctttttagtt gttagtggct
     2821 agttgttagt ttttagtaga tggctttatt ttaaaacttc taactgcaaa ctacaaactc
     2881 acgctattag cctgctctgg tttcggtgag tttctggatg tgggcgacga cttcgtcgat
     2941 cggtttggcg cctaaatcgc ctttggagcg gtcgcgcagg ccgagggtgc cgttttccat
     3001 ttctttatcg ccgacgacag ccatgtaagg aactttttcc atctgagcct tgcggatctt
     3061 gtagccgatc ttttcgctgc tttcatcgac ttcgacgcgg atgccgaatt tcttgacctt
     3121 cttagccagg tctttgcagt aagccaggtg tttgtcggag atgggcagga acttgatctg
     3181 gaccggagcc agccataccg ggaaagcacc ggcatagttt tcggtaatga tgcccaggaa
     3241 acgttcgata ctgccgaagc aggcacgatg taccatgacc gggcgatgtt tctggccatc
     3301 ttcgccgatg taggtcatgt cgaagcgttc cggcatgagc atgtcgagct ggatagtacc
     3361 gcactgccag gtacggccga tggagtcttt caggtggaag tccagtttcg ggccgtagaa
     3421 tgcgccgtcg ccttcgttga tgacgtacgg gatgtggcgt tcttcgatga catcatgcaa
     3481 ggtcttggta gccagttccc agatttcgtc ggagcccatg gagtcttccg gacgagtgga
     3541 caattcgacg tgatattcca ggccgaactg tttgtaagcc ttgtcgaaga ggtccatgac
     3601 gccggagatt tcttctttca tctggctcgg cagcatgaag atgtgagcat cgtcctgagt
     3661 gaagcagcgg acgcggaaca ggccgtggag ggccccatgg agttcatggc gatggaccag
     3721 gccgagttca gccatgcgca tcgggaagtc gcggtagctg tggacgttac tcttatagac
     3781 caggatgccg cccgggcagt tcatcggttt gatggcgtaa ggctgttcat cgatggtcgt
     3841 gaaatacatg ttgtcgcggt agtgatccca gtggccggac tgttcccaga ggtgacggtt
     3901 gaggatcatc ggggtcatga tttcgtcgta atcggcttca tcatggagtt catgccagaa
     3961 tttaagcaag ttgttgcgca ggatcattcc cttgggatgg aagaacggga agcccgggcc
     4021 ttcttcgtgg aagctgaaca ggtcgagctg tttgcccagt ttacgatggt cgcgcttttc
     4081 agcttcttcc atcatcttca ggtattcatc gagttccgat ttcttttcaa aagcggtccc
     4141 gtaaatacgc tggagcatct tgttgtgttc atcgccgcgc cagtaagcac cggcaacggt
     4201 gcgcagcttg aaggccttga cggcaccggt cgaagcgcag tgcgggccgg cacagaggtc
     4261 gacgaagtcg ccctggcggt agatggagat gacggcatct tccggaaggt cttcgatgag
     4321 ttctacttta taatcc
//
LOCUS       DAWCYS010000127         4313 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1120_length_4313_cov_5.470174, whole genome shotgun sequence.
ACCESSION   DAWCYS010000127 DAWCYS010000000
VERSION     DAWCYS010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_4313_cov_5.470174"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1276 a         1042 c         1065 g          930 t
ORIGIN      
        1 tggaactcaa gaagctcggt atggcattgg ggacgaaaag ggagtctttt tcactgaaag
       61 acaacgagaa aaaccaattc gccaaaaaac aggcctattc gctccatata ggcgatgaaa
      121 attctaagcg gctaactata tctaaacatg agctaacgaa gctcacagag gcatatagaa
      181 gggcgaacat atggaagcgg acggcgaaag agatagctct cgtcgtggat aaggaaaaat
      241 cgcagacacg aaaggaaaca gccgaagata tcgccgtaaa ggaacggcct atcaaggctg
      301 ttttcatcaa tccgtgggaa gcagtaatca ttgtcgacga tgcgacggcc tttttcaact
      361 ggttccgttc ggtacaggaa acgttgtcca ttgcggagca tcacaacaag ctcatacacg
      421 ccacgaggcc agaatcatta cggataaccc tggcactccc aagaaaattg tcacgggcgt
      481 tccaggaaac cataaccgct atcgaacgat atatgagcca cgcacaaccg aaatatgatg
      541 aaatggcagt gacattcacg gacggcgaaa acaaagccct tgaggttatc cgtagtgaaa
      601 tgattcatac gactgaaata tactgggaca atgtgttgtt cctcatccat atttcagagg
      661 atatccggac cacagaagcc gtgaagaaga cggccgtcaa acagctggac ggcgccttta
      721 gcttcatgga ttcattgcgt agagcatccc gattaaacaa agtggaggac ctggctatta
      781 tcgaggagaa acggcaggct atagaacatg agctgtatga cggactgcat atcatggacg
      841 aatggcggaa tgatatgcgc acattggcgc gtgaacaggt aaaggccatg gactacatca
      901 ccaaacatgc cacgtcggcg cagttcgatg gattctatgt gcgggacggc tataaaaaga
      961 tatggcaggc cgtgcaaacc ttccgggaag agctgaaaac actcgataag gtgtaccgga
     1021 atatctacgc cattcgtgat gatcgtatcg ccatcgccga taaacggcta gtgaaactcg
     1081 aacgcaccct gcatgaagct gtgggaaccg acgagtcctt taaccgggat atggttttca
     1141 ttcgtggatt cgaggacctg gcccgtatcg gcgacggcct aacacgggat gtaggcaaga
     1201 acccggccga agatattgcc ctgtacgacg cgttcgtaag ggccagtaac tcctacgtcg
     1261 agtcggtgca ggtactctca tcgttcaagg aaatggacga ctttacagcc atgtcggata
     1321 ccccgccact gtacgaacag ttcacggact tcaacgtagg ggactacgaa tacgaaaagg
     1381 ccctcttgcg gctccgtgtg gtcagtaagg ccacgcaggc acagcctttg ttgtacgatg
     1441 tatcgccgca cgttgatatc gacgataccg atgataaggg gcagcttgaa atcaaggata
     1501 cgacagcggc cacgaaagtc tattacaaca agcattacta taacgccccg gaagtcaacg
     1561 ccatggtcaa gggcggtaca ggcacgacga cgtccgtacc gaatatcctc acgaccgacg
     1621 ggcaggacga caaagggcgt tactttgaaa tcgaattact gaatagttcc ggcaaccgta
     1681 caacgggcat cgtctcgtgg gttgcgaaag gatggtaata tgcaggaata taacgaactg
     1741 gtcacgacgg acgcctgtaa tacgtacctg gagaaagcgg acaagaacat tcagtccgta
     1801 gccagtacgt tctccggcac ggcgttccca acgacgaacc ttgctgtcgg gatgcagtgt
     1861 atgcgtaccg acgacagcaa caatatctat aaattgacgt ctacaagccc ggtaacgtgg
     1921 gaattagtac cgtctaaatc ttacgtcgat aatgctgtca ctaccggcgt gaagagtgtt
     1981 acgaatttca agggggccac ctcgaccgcc gacggtgcgg ctggccttgt cccggccccg
     2041 gccaaaggga cgcagaccga ctactacctg tcggcagatg gcacatggaa gaaggtacag
     2101 caacgtactg ttaaagaagt catcgacatc gtccatcctg tcggcagtat ctgggaaacc
     2161 acgacgaccg acgaccccaa taccttatgg gctggtacga catgggtcaa gatggatgca
     2221 ggccgcgtac tggtagccac tggcagttac accgaaagcg gtacgacgta tacttataac
     2281 ctcggcgata caggcgggga agcgaagcac caactcacta ccgaggaaat gccaaatcac
     2341 aatcatacag ccacttgttc tacaaatggt aatcataacc ataaaatacc aaataacaat
     2401 gatagtggtg gaagcggtaa ctacattgac gggggtgata gtacaaatat tgtgttgtat
     2461 acaaacacta gttataacgg cgaccattca cactcaattt ctattggcta taccggtagt
     2521 cacatagcac atgaaaatag gcaaccattt tgtgtagtta atcgttggaa acgtattgct
     2581 taaaccgttc tcttccaacg gttgataact tgatatggct gtctgttctc atgactattt
     2641 ccaccaccag cattattgat gccgatttga tgcgaatgtt cgccgttttc actagtggac
     2701 cagtcagcgc cagcggtgcc cacccagtca gccgactgac cagagccact ggctctaact
     2761 aaatacgtat gcgtatgctt accaacagca ctcatccacg ccccatgagt gtgagagggc
     2821 atttcctcgg tagagaaagg agattttatg agagtttttc agattttaga taacgacgtt
     2881 cttatcatca aggacaataa gcagtacacc gatactgccg aacatttcaa ggcagacagc
     2941 ggcctttcca gcctgcctgc taaagtaatt tacgacgaca cccagaaaca gtgccttgtg
     3001 gatgatgatt ttaaagacta tcccaacgct gaatatgacg gctatatcga caacgtaacg
     3061 gcctatatcg aagcgaaagc gaagcgggaa tatgtaccgc ccacgctcag cgaattgaag
     3121 gcgcaggcgc tcaacgtcca gtacagcaag taccttgcca agaaggaagc ccctgtcacg
     3181 gtagatgacc tgcaattcag caccgacgaa aacagccagc gcgaatggca gattgctctt
     3241 accttgatta gtgataaagg gccgtataaa gtccatgact cgtccaacag cctcgtattg
     3301 gctgatgtaa caaaagaaca gctcatgaag gctggagaag ccgccagagc gcaacagctt
     3361 gcggcttatg aatggttcat gagcatccgc gatgccatca ataattgcaa gaatgaagaa
     3421 gaacttgcgc cgtacatgac ctaacgaacg gcatggttaa gccattcttg catgatttac
     3481 gataaagaac acataaagag cagtgatgaa ccgcgtagtt tagccgttat cctgctcttt
     3541 tctattgaca taaaagaaca cgttaagaac agaaggtgat tgtaacgaat gagtaatgtt
     3601 gcaatagatt tgactaaaac agtaaaagaa atcatcacga cgatgaagac ggctatcgac
     3661 aatctcaact ccgacgtggc gaccaataag acggctatcg acaatctcaa agagcagatt
     3721 ctggaggccg tgtatcccgt cggctccgtc tacgtgagca tcacagacag ccgcaatcca
     3781 gcagatattt tgggcttcgg aacgtgggaa gccctgccag caggatatgg actcgtagca
     3841 caaggcacgg ctacagcaga agacggcagt acgctcacat ttacagcagg acagaagtcg
     3901 ggcgaattta agcaccaaat cactgtcgga gaattgccgt ttaacaaaag aattgctaca
     3961 gacgatggtt ggcacccagt gtggggaaat gacgcaaatg tacagtctct tggcctcaat
     4021 aaaaagcagc atattacaca aacacccttt tctataatgg gccctcaagt aggggcctat
     4081 atttggaaac gcattaccta agctgttctg cgccagctat gctttgcgtt tccaaatata
     4141 ggaagctaag cacggcgata cattgttgtg tggttggtct ccccctgtgc tctcgataat
     4201 gtcagtccat ctatttgtgg ttgtatcact cttaaccgcc gcatcatgca caataccagg
     4261 cagattatag gccccaccct taccgtcaga agagttccag tcatcgttgt aaa
//
LOCUS       DAWCYS010000128         4311 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1121_length_4311_cov_5.592810, whole genome shotgun sequence.
ACCESSION   DAWCYS010000128 DAWCYS010000000
VERSION     DAWCYS010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_4311_cov_5.592810"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          882 a         1256 c         1192 g          981 t
ORIGIN      
        1 agcccgttca acgcatggct cctcatccag ggcctggaat ccttgtctct gcgcatcgaa
       61 cggcacgtcg ctaacgccct gaaaatcgca gacttcatgg aaaagcatcc gaaagtcgct
      121 aaagtcaact atccgtccct cccgtcttcg ccgtactaca aactggctca gaaatacttc
      181 cccaagggaa ccggttcgat cttctccttt gaattgaaag acggctatgc agctgcccgt
      241 aaattcatcg acaatgcgga catcttctcc gacctggcta acgtcggcga ctccaagtcc
      301 ctcctcgttc acccggcttc gacgacgcat cagcagcttt cggaagaagc tcagcgcgcc
      361 tgcggtatca caccgggcac gatccgtatc tctgtaggcc tcgaaaacat cgaagacctc
      421 ctcgccgctg ttgaaaaagc actggaaacc atctaagact attgtaagga gaaatgcact
      481 atgcctatta aaattgctga cggtctttcc gccaaggaaa aacttcacga agaaggtatc
      541 ttcaccatcg ataaggggct ggccctccat caggatatac ggccgctgcg catccttatc
      601 ctcaacctga tgcctttgaa gaaaccgaca gagctgcagc ttctccgcct cctcggtgat
      661 tcaccgctcc aggtcgaagt ggacttctgc catacggaca gccatgagag cacccacacc
      721 gacccgtctt atttagaaga aaactactac gtcttcgatg aaatcaagga taactactac
      781 gacggtttca tcatcaccgg cgccccggtc gaacagattc ccttcgagtc tgtcgactac
      841 tggccggaaa tgaagactta ctttgaatgg gccaagacac atgccttctc gacgctccac
      901 ttctgctggg gcgcccaggc agccctgtat tactactatg gcatcgaaaa gcgtctcctg
      961 ccggccaagc tcttcggtat ctttgaatac cagctgaccc agaagcacca tcccctcctg
     1021 cggggcttcg atgaccgtta cttcatcccc cagtcgcggc atacggcgat taacgacatc
     1081 caagtctaca atacgccgca gctcgacatc ctctcacggt ccgtcgaaaa cggcatcaac
     1141 atcatcggta cgccggatca ccgccgtttc ttcgtccttg gtcacttcga gtacgaccgg
     1201 cggaccctgg aagaggaata cctgcgggat aagaaaaagg gcctgcccat tgccgtgccc
     1261 aagaactact atcctggtga caacgatacc aagcagccgt acttcacgtg gtgcagctac
     1321 gctcacctgt tctatcacaa ctggctgaac atcgtctatc aggaaacacc ttatgattta
     1381 caagccatcg ccaaggtcca tccttgtgcg cattgaaaaa aggcctgtcg catttgcgac
     1441 aggccttttt tacaccgtag ggggcgccac gtgggcgacc cgttgtgaat gtggatgatt
     1501 ctctaaactg gacagaaaat cacacagatt ccagggcggg ctcgccacgt gcgagcccct
     1561 acaataaact acaaacgatc tttattttcg ctaaacgacc tacaatgaac gattacttcg
     1621 ttccgaagat gcggtcgccg gcatcgccta agccggggac gatgtagcct tggtcgttga
     1681 gtttatcatc gagggcagcg acgtagatgc ggacgtctgg atggtcggcg ttgactttct
     1741 ggacgccctg gggcgctgcg acgaggcaca tgagcttgat gttctcgatg cctttttctt
     1801 tgagcagcgt aatggcagcc gatgcactgc cgccggtagc cagcatgggg tcgacgatga
     1861 tgaaatcacg gtcttctaca tcgggcagct tgcagtagta ttcgaccggt tccagcgtct
     1921 gcgggtcacg gtagaggccg atgtggccga ctttggccgt cgggatgagg ttgagcatgc
     1981 cctgtaccat gcccagaccg gcgcgcagga ccgggacgat gcccagtttc ttgccgctga
     2041 tctgtttgcc tatcatcttg gcaacaggcg tttcgatttc gacgtcttcc aagggcaggt
     2101 cccgtgtgat ttcatagccc atgagcatgg tgatttcttc caggaggttg cggaaatcct
     2161 tcgaacccgt ttcctttttc cgcatgagtg tgactttgtg ctgaatgagc ggatgatcca
     2221 ttacttgtac gtgcatgtgt gaaatcccct ttcctattta tccatttctg caatcttggc
     2281 gacgcggaca gcgtggcggc cgccatcaaa accagtggag aggaagatac ggacgatttc
     2341 tctggccagg cccgggccga tgacgcggcc gcccatggtc aggatgttgg cgtcattgtg
     2401 gcggcggcag tattctgccg agaagcagtc gtggcagaga gcggcccgga tgccgtggac
     2461 tttgttggca gcaatgccga tgccgatacc tgtgccgcag acgaggatgc cccggtcgaa
     2521 agtgccgtca gcgacggctt cagcgacttt cttggcaatg tccggataat cgcagcgttc
     2581 gcccgtcagg gtgccgtaat cggtgaattc aatgccttgt tccttcaaat attctttgat
     2641 gttttctttc aggtcaaatc cgccgtgatc ggcgccgagt actaatttca taatcaattc
     2701 ctcccatata tgctgataaa ttcagtttta ttatacccct ttcggagtca tgtgaaaagg
     2761 tttaaccgac aatgaccacg tggccgccgg cagctttttt catgcggttc atgatagcca
     2821 ggcccaatcc ctcatcgtcg atgccttcgg ccaggatgat atcgacctgg tcgctgtcaa
     2881 aggacaagag gcccgtgtag agctggttgg ccagggcttc cttgtcgcca tggcgacccc
     2941 agatatacat atctttatgt gggaacgagg cccctacttc ttcgctgacc aaaaagccga
     3001 cggttttgcc ttcgcccttg gcctggtcgt ataaggcctg cagtttttcc gtcaccttgt
     3061 cgacagggcc gacgacgacg gtcatcggtg ccgatggcgc atagtggcgg tatttcatgc
     3121 ccggcgcctt ggggacgccc ttgcccgtga ccagcgtcgt atcgagctgg acgtgaggta
     3181 cgacttcctg aagcatctcg acagtgacgc cgccgggacg caggatggtg acgccgtcgt
     3241 cgccgttaca ttcgacaatc gtcgattcga cgccgatgtg gcagggaccg ccgtcgatga
     3301 ccatatcgac gcggccgtcc atgtcgtgca tgacttcttc ggccgtcgtc gggctgggcc
     3361 gggtcgacag gttggcgctg ggaccggcta cggggacgcc ggcacagcgc aggaaggccc
     3421 ggcagatatc gtggtccggg cagcgcaggg ctaccgtatc aaggccgccc gtcgccgcat
     3481 caggcacgat gggcttgcgc ggcaggacga tggtcagcgg tcccggccag aagcggtcca
     3541 tgagcttctg tgccgtcggc gggatgacag ccgctgcctg gcgggcccct tcttcgtcgg
     3601 gcacggtcag gatgagcggg ttatcgctgg gccggccttt ggcgacgtaa atgctgcggc
     3661 acgcgtcggc gtcgaggcca ttggcaccga taccgtagac cgtttccgtc ggaaagacga
     3721 ccaggccgcc gtcgcggatg atctggcccg cttcttcgag gatggctttc tgtttctcta
     3781 tatccgtaat ttctacaagt ttcgtctgtt tcatcgtctc tactcttttc tggaaaatac
     3841 gaggaccctg tcgatgccac cgtaatcctt gacggtctgt tcgtggtcat aggcgccatg
     3901 gcggcagaaa acagccgtca cggcgtcggc ctggccggcg ccgatttcga cggcacagtg
     3961 gccgccggct ttgacgtaag ccgggctttc tgcggcgatg cggcgataga attccagtcc
     4021 gtcgtcgccg ccgtacaggg ccagggccgg gtcgtggcgc acttccggct ggagatgttc
     4081 catatcgccc ttggtcaggt acggcggatt ggacaccagc cagtcacaag cgccttgcgg
     4141 aaccttggtg aataaatcgc tttcgatcca ggtcacgcgg ccgtctaggc ccagggtacg
     4201 gccattttca gcggctatgg ccagggcttt gggcgaaatg tcgacgccct tccccgtggc
     4261 ctgcggcaga tagtggagca ggctgaggat gatggccccg ctgcccgtac c
//
LOCUS       DAWCYS010000129         4296 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1125_length_4296_cov_8.899552, whole genome shotgun sequence.
ACCESSION   DAWCYS010000129 DAWCYS010000000
VERSION     DAWCYS010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_4296_cov_8.899552"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          939 a         1069 c         1324 g          964 t
ORIGIN      
        1 gcaccagtgg gaagaaccct gtatcagcgg cagccacggc tctggcgccg tcttcttcac
       61 gggctgcaac ctgcgctgct gtttctgcca gaatgccacc atttccatgg gcgggacggg
      121 cgtgcccatt acggtggaca agctgcggga gctttacggc cggctcatcg cccagggggc
      181 gcacaacatc aacctggtca cgccgacgcc gtataaacag gccatcttgg cgagtctggc
      241 cgcccccctg ccggtaccgg tcgtctacaa ctgcggcggc tatgaatcgc aggagacgat
      301 tgacgatttc aagggcaaag tgcagatcta tctgcccgat ttgaaatacc tcgacccgaa
      361 attggccaag cgctacagtg ctgccgaaga ctatccgcaa gtcgccacgg cggccatcct
      421 gcgcatgtac gaccaggtcg gcccttatga aatagacgac gacggcatcc tgcagaaggg
      481 cgtcgtcatc cgccacctca tcctgccggg ctgcgtagac aatacgaaag ccgtcatcga
      541 ctgggtgcga gaccacttcg atgacggcca ggtcttattc agtttgatgc gccagtatgt
      601 accctgcggc cgggccgggg aataccccga aatcgaccgg cccttgtcgg acgaagagta
      661 cgatgaagcc gaacagtacc tcttcgacag cggcatcgaa gacggcttcg tccaggaaaa
      721 aggcagtgcc agcgacgcct tcatcccggc cttcgacggt acaggcgtcg tctaagacgg
      781 gctgcccttg ccccagggga cgcggatgta cgtagagaac cagccgtctt cacaggtgca
      841 gaggacgctg gcgtcatagg tgtcgcggaa gcggctcagc gatttcatgc cgatgccatg
      901 gcccttgacc tttgtcgtcg gcaggccgtc ttcatcgagg gctacggtcc cgtcgaagcg
      961 gttcttgacc aggatgctga ggatgtcgcc ctggcagatg gtcagcaggg acagggcgcg
     1021 atggcttccg ggctgctttt cgctggcatg gatggcattt tcgatgaggt tggacaagac
     1081 gatggccagg tcggagctga gagccgtatt ttccttgggg atgacggctt tgacggtaat
     1141 gggcatgtgc agggcttcgg ctttggccag atacgtcgac aaggcggcgt tgaccagggg
     1201 attctgacaa tactgtttgc gcttggtctg attcagctcg gcaccgattt cctcgatgag
     1261 ctgcaaggct tcttgggaac gatgggacac gatgagttgt tccagttcgt ccaggtaggc
     1321 cttgcgtttt ttatatatgg ccttcatctg gttctgtgac tccagcaggg aatgggtata
     1381 atcgtagctg gcttcgattt gggctgtcat attttctgtc cgttcgtaca tcttcagggt
     1441 ctgatcggtt tagcggaggc ccgtgcggat gctctgggcg atgatgagca aagccaggag
     1501 caggatactg cggggcagga gcatccggtc catgatgagg acgtccgaac tggccatgat
     1561 gaagtacatg tccagcagca ggaagagcgg cagcatgctg atgtatttcc aatagtgatg
     1621 cgtcgtcaga tgacggtatt tcacgaagac ttcattgaaa taacggcgca gcatgggcat
     1681 aagggccatg tagcagacga tatagaacag ccattgggcc ggatagatgg ctgccgacag
     1741 ggccggccgg gagctgtcgc catggaagat ggccagcagg atcagggcca tcagggtgtg
     1801 gacgaagatc gtacaggctg cgcggatgga aaagacgtag agatggagga atacataagg
     1861 ccggatcgtc acgacggccc aaagaaaatg gggaatccag gcgatgacaa agactttttt
     1921 atataagatg aagctgtgtt cccagggacc ccagacgaac atatagagga aaaagatgag
     1981 ttccatgagg tacaggacgg catatccacc caggacgagg actttctgcc gatgcttcag
     2041 caagggttca aaggcatagt actggaggat agctggcggc atgagggctg tcgtcataag
     2101 gaaaacggtc acgatgttgt aaagcatggc tgagtatcac ttccaatcaa gtctgatagg
     2161 gaatcgtcaa atacaggctg gccaggtggt tgtggaggtc atagcggaag gtcgcctcgt
     2221 agagccggga gaactgattc aggtccggaa cgaactgggc atagggcgct tcccggagga
     2281 ggtcgttttt gtcgtccggg atgtccggtg atacggcatg tttaatgaag accagcaggc
     2341 cgccgtgtat gggcgtcgtc ccgatgatga gcaggcgctg gtctttcggc agggactgga
     2401 gggcctgggc cgacatggca atggctttgg ccaggatgtc gcccaaggcg atttcaaagg
     2461 gcagggacgg ggggaagccg tcgtcaagac cgtagctgac atgcatatca ctccctttct
     2521 atcaaataca agatacctta ttatggcata tttatgtggc tggcacatgg atgtttctga
     2581 taaggtgcat tataatttgt aggggctcgc acgtgccttt tcagcccgtc aggccatgac
     2641 tacaaggaat ggcaggacgg ttgctgaaac ggaccactgt agagggcgag cccgccgggc
     2701 atatgtaaga atcccgtggt cgatttgcgt ggacgggcgc cccacgtggg cgcccctaca
     2761 gaaaaaagga gctgtcgcga tgcgacagct ccttttttac atcgccttga ccggtacgag
     2821 tcctcgtgag agggaaatgg tcccggtccg ggcgatttct aatatttcat atggcgacaa
     2881 taccttgatg agggcattga cgtggtcttc gcgtcctgta acgcggagga tgagcgagtt
     2941 caagttgaca tcgacgactt tggcgtggaa caggttggcc aggctggcca gctcgtcgcg
     3001 gacttgcggc gtgtcgtagc ggaccttgat catggccagt tcgtagctga tgaagggcgc
     3061 ttcatcgagg ttgatgatct tgatgacgtc gatgagcttg gccagctggc tgatggtctg
     3121 cttcaattct ttatcgtctt cgatgctgat gacgatattg aagcgcgaca catctggttc
     3181 ttccgtataa ccgacggtca acgattcgat gttgatggcc ttgcggctga tcaggccagc
     3241 aatatgtgtc aatacgcccg gtttattatc ggacaaaata gccagatgct gttttctcat
     3301 caggcgtcac ctcctaaaat catgtcactc aatgctttgc cgccgggaac tgtcgggtat
     3361 acccgttctt ccggcgagat gcggatatcg accagggccg gccctttatg ggcaaaggcc
     3421 tctttcagga cgggggttaa atcgtcctgt ttttcgatgg cgtatccttt gacgcccagc
     3481 gattcggcca gcatgggcag gtttttgcgc ttattcaaag tgcattgtga ataatggtgg
     3541 ttatagaaga aatcctggag ctggacgatc attcccagcg tatcgttgtg caagacgatg
     3601 actttcacgt cgatgccgta ttccgacaag gtcgcgaatt cctgacagtt catgaggata
     3661 ctgccgtcgc cggaaaagac gacgactgtt ttttcgggac aggccacttt ggcaccgagg
     3721 gcagccggca ggccgaagcc catcgtgccc agaccaccgg aagaaatgaa gtgtttcggc
     3781 tcatccgtcg tgaagaattg agctgcccac atctgatgct gaccgacgtc ggtaatggct
     3841 acggtatcgt cgtcccgggt gatgtcccgg acggcggcga agattgactg gggtgaaatg
     3901 cagcctggac gcggcggcgt cgcgaagggg tggcttttct tcatggccag gacctcatga
     3961 cgccaatcgg caaaggcggc tccataatcg aaggcgcctt ctttcagctt cttggcgaaa
     4021 atgggcaggg accaacgcag gtcccctttg atgcctaagt cgatgtcgat gatcttgtcg
     4081 atttcgacat catcgatttc aaaatggatg atctgtgcat tgggagcaaa cgtatcggct
     4141 cggctggtca ggcgttcgtc gaaacgggcc cctaaggcga tgagcagatc gcagcctgag
     4201 atggctttgt tcgatgcata agagccgtgc atcccgccca taccgagatg aagttcgtga
     4261 taatcgggga tggctccttt gcgcatgatt gtcgtt
//
LOCUS       DAWCYS010000130         4289 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1128_length_4289_cov_8.293812, whole genome shotgun sequence.
ACCESSION   DAWCYS010000130 DAWCYS010000000
VERSION     DAWCYS010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1128_length_4289_cov_8.293812"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          900 a         1122 c         1253 g         1014 t
ORIGIN      
        1 gaatacctct ttttaaaaca atatgtttat aagtccaaaa aaaaggaacc tcctgccccg
       61 atggggattc tccgccattc cgaagaggcc cggccagggc aagaggctcg ttgaaagact
      121 aattatttca tgacagcgcc ggtattggca gacgtgacga gtttcgcata gcgggacaag
      181 taaccggtct tgaccttcgg ttccggctgc ttccattcag ctttgcgttt tgccaattct
      241 tcatcgctga cttccagttc caatttacgg ttgggaatgt cgatggaaat agtgtctccg
      301 tcgtggacca gggccagggg gccgccttcc atagcttccg gcgagatgtg gccgatgcag
      361 gcgccgcggc tggcaccgct gaagcggccg tcagtcagga gggcgacttt caggcccatg
      421 ccggtgatga cagctgtcgg gttgagcatt tcacgcatgc ccggaccgcc tttcgggcct
      481 tcgtagcgga tgacgacgac gtggccttct ttgatttcac cgccgatgat agcggcaata
      541 gcagcttctt ccgagtcata gcatttggcc gtacctttat agaccaggag gtccggttcg
      601 acggcacttt ccttgatgac cgagccgaga ggcgccaggt tgcctttgag gacggcaatg
      661 ccgcctttat gcatataggc gttttctacg gaatagatga cgtcgtggtt gaggatcttc
      721 gcatctttga tgcggtcgcc gaccgtgccg gtaatggtct tggcgtcggt gtggatgagg
      781 cccaatttgg agacctcttt catgacagcg cagatgccgc cggcttcatc gaggtcctgc
      841 atatgatacg tgccgccagg gctcatcttg cagatatagg ctgctttacg gctgatttcg
      901 tcgaattcat cgagcgagag gtcgacgccg gcttcgtggg cgatggccgg taaatggagg
      961 gccgtgttgg tcgaaccacc gatagccatg tcgacggcaa tggcgttttc aaaggcttcc
     1021 agggtcatga tgtcgcgcgg cttgatatct ttgcggacca aatccatgac gacctggccg
     1081 gctttcttag ccaacatgcg gcgcaggccc gtataggcag cgggaatggt gccgttgccg
     1141 ggaaggccca tgccgaggac ttcggtcatg cagttcatgg tgttggctgt gaacaagccg
     1201 gaacaggaac cgcagcccgg gcaggcctgg aattccatct ggtgcatttc ttccggtgtc
     1261 atctggcccg attcgacttt gccagcggct tcaaacatgg tgctgacgct gatgttcttg
     1321 ccgttgtgac ggccggcgag catggggccg ccgctgacga cgactgtcgg gatgttgagg
     1381 cggccggcag ccatgagcat agccgggacg attttatcgc agttcgggat gagtaccagg
     1441 ccatcaaagg catgagctgt cgctacggct tcaatggagt cacagatcaa ttcacggctg
     1501 gacaaggaaa agctcatgcc gcgatgaccc atggcgatac cgtcgcagac gccgatggac
     1561 gggaattcaa tcggtgtacc gccggcagcg gcaacgccga gcttgactgc ttcggcaata
     1621 tcgcgcaaat ggatatggcc gggaatgatt tcattgaagg cattacagat accgatcaac
     1681 ggtttctgca aatcttcatc gctatagccc atagcatgga ataacgtacg atgggcggtt
     1741 cgggtaacgc cctttttaac aacatcactg cgcatggtat tcatcctttc tctacatatc
     1801 cttgacggcc atttcgccgc gggacaggga aatctgcccg gtccgggcga tttcaataat
     1861 atcataatct ttcagcattt ccaaaagagc ctcgatatgg tcttcccggc ctgtcaggcg
     1921 caggaccagg gaacggcgct ggacgtcgac gatcttggcg ttgaagagct gggcgatatt
     1981 ggtcagttct tcgcgggtct gcggcgtcgg gctctggact ttgatcatcg ccagttcata
     2041 gctgatgaac ggcgcatcgt cgaggttgac gactttgatg acgtcgatga gcttggccag
     2101 ctggttgacg gcctggtcga gttcccagcg gttttcaacg gaaacgacga tgttgatacg
     2161 cgttacgtca gcttcttccg tataaccggc attgatacat tcaatattaa tggctctgcg
     2221 gctgatcagg ccggcgacgt gcgtcaggac gcccggttta ttgtcgcaga gaatagcgag
     2281 atgttgtctc atcatttccc ttcacctccc aataccatct ggtccaggcg cttgccgccg
     2341 ggaaccatgg gatagacatt ttcatcagcc gggatgaaga cgtcgagcag tgccggccct
     2401 tccgtcttga acagggcttc tacggcttta gccaggtctt ccggcttatg gacggccgtc
     2461 ccgggaaccc ccatggacgc ggatacggct gtcaaatcgg ggttatcccc aatcagcgat
     2521 tcagcgcagt ggccgttgta gaacaagcgc tgccactggt tgaccatgcc caggacgttg
     2581 ttgtggagaa cgatggtctt gacatcgacg ccatatttat gaagagtggc gaattcctgg
     2641 cagttcatca tgatactgcc gtcgccagtg aataagacga ctttcgactt gggacagccc
     2701 agcttggcac cgagggcagc cggcaggcca tagcccatag tacccagacc gccagaggta
     2761 acgaagcggc ggggcttatt taaatcgaag aactgagctg cccacatctg gtgctggccg
     2821 acgtcggtaa cgacgatggt gtcgtcgtcg gtccacttct ggacctcttc aaagagcttc
     2881 tgcggcgaaa cggcatcggg aaggtcccgc gtggcaaagg gatgagcggc cttcatatcg
     2941 aggacgaaag tccgccattc accatagcgg tccgccagat gcgtttcatg agctgccagc
     3001 ttttcgtcga ggagcggcag ggaccagcgg aggtcgccgt tgacgctgat gtcggcgtgg
     3061 acgattttat tgatttctac tttatcgata tcgaagtgga cgatctgcgc gttcggcgcg
     3121 aaattttcga cgtcgccagt gacgcggtca tcgaatcggg cgccgatggc gaccaggagg
     3181 tcgcatttcg taacggccat attggcagcg tacgagccat gcatgccgac catgcccagg
     3241 tgggcgtcat acgtatcggg gacgcagccc ttgcccatga gggtcgtgac gacaggaaca
     3301 tgcatctgct ggacgatgtg cttcaagcgg tccgacatgt tggaaaggac gacgccgcca
     3361 ccggtgagga tgaccgggcg ctttgccttt tcaatggctt cgacggcgtg gtcgacatcg
     3421 gcttcattgc cgacgtgatg gccctggtag ccgcgcagat ggacttcttc gggatattca
     3481 aattcaaaag gtgtatcgaa tacatccttg gcaatatcga caaggaccgg cccgggccgg
     3541 cctgtggttg cgatgtaaaa ggcttctttc aagacccggg gcaattcctt gacgttcttc
     3601 actaaatagt tgtgtttcgt aatcggcgtg gtaataccgg agatatcagc ttcctggaag
     3661 gaatcgcgcc cgataagggg cgttgctacc tggccgctga tgacgacgag gggaatggaa
     3721 tccatgttgg ccgtggcaat gcctgtaacc atgttgcaga cgccaggacc ggacgtagca
     3781 aagcagacac cgggcttgcc cgtagcgcgg gcatagccgt cagcggcgtg gacagctccc
     3841 tgttcgtggc ctgtcaggac atggggaaat ttggctttat ataattcatc atataatgtc
     3901 aggacagccc cgccgggata gccgaagacg acttgtacct gttcacgttt caagcattcg
     3961 atcactgctt gtgctccggt cattttcatc tcattcacct catcataaaa catgactcat
     4021 acttacaaag aagctattct tcaatcaacc tgcttcttgg gtttgatgtc tgaagtttga
     4081 tgttaaaagt tatttccaac ttcaaactcc aaacatccaa cttcaaacta tgtcttagtc
     4141 gttctggtcg ttgttgtgga gccacggcat catggcacgg agtttttcac cgacctgttc
     4201 gagcggatgt tccgcatgct gacggcgcat agccaggaag tgagcgcggc cgccggcttt
     4261 gttttcgagg agccaatctt tggcgaagg
//
LOCUS       DAWCYS010000131         4239 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1142_length_4239_cov_5.512189, whole genome shotgun sequence.
ACCESSION   DAWCYS010000131 DAWCYS010000000
VERSION     DAWCYS010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_4239_cov_5.512189"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          853 a         1131 c         1216 g         1039 t
ORIGIN      
        1 ccctttatag gggagctggc cgccaaaggc ggtctgagag gttaaaggtc ggcccgctac
       61 gcgctgaggg cttcccgaag cgtcgttgtt agtttgagtc tgtcgcggcg gaacgccgca
      121 tctgaatccg cgagaaaggc cgtggttcat tattcgtggc tcgtaaaaac atacccatta
      181 tactcgaatg ggctgttatg aaatctctct ggcgggcttc ccacgggaag cccctacagt
      241 tcggccatgg gccgtgtgta tttttgcgaa cggctaacag ctagctgctg actgccaaaa
      301 aggcgctgtc gcatgtgcga cagcgccttt ttcactacaa acttgatact gcaaactata
      361 ttctatttat cgatgacttc cttgacgatg ctgatagctt ctttcaattc atcccagggg
      421 atgttgagcg gcggcaggag gcgcaggagg ccgttggccg tgaggacgag gacgccgtgg
      481 tcgacgcagc cttctacgac gtccatgagg gctttattct tgagggtgac gccaatcatg
      541 aggcccatgc cggatacgtg gtcgacgtgt tcgcactgtt ccagttcttc tttgatgtat
      601 ttcccttttt tccggacttc ttccatgagg gcgtcgtcga ggcggtcgat gatggaaatg
      661 ccgccggccg tgcaaatcgg gttgccgcca aaagtcgaac catgggtatg gttggtcagg
      721 gtatgctgcg tcgaatcggc gaacatcgta gcccccaagg gcaggccgcc gccgatggct
      781 ttagccgtcg aaatgatatc gggctggatg ccgtagccca tgtaggaata gagatagccc
      841 gtccggccat taccggtctg gacttcgtcg acgataatca ggatatcgtg ggcatgcgca
      901 tattctgcga cctgctggac atattctttg tcgaggggat ggacgccgcc ttcgccctgg
      961 accatttcca tcatgatggc gcagacgtta ttttcttcgc atagccgctt catatcggca
     1021 aaatcatcgg gttcggcata gacgaagccg tcgacgaagg gcccgaagtc ctcatggagc
     1081 gaatcctggc cgcacgccgt caaggtggca atggtccggc cgtggaagcg gttcttcaag
     1141 gtgacgatgg tatagcggcc gtcgccgtat ttgtcgtgcg aatacttgcg ggccgtcttg
     1201 atggcacatt cgttggcttc ggcaccggag ttcgagaaga agaccttctt catacccgtc
     1261 ttctggcaga ggttttcggc caggacggtc tggggttccg taaagaagac gttggaaata
     1321 tggctcaatt tatggagctg cgcttcaacg gctttgatcc acacgtcgtc acagacgccg
     1381 aagatgttga cggcaatacc ggcgctgagg tcgatgtatt tcttgccgtc cacatcatac
     1441 aaaattgcgc ctttgccgtg gtccaatacg gcaccgaccc ggttgtatac attgacgata
     1501 tattctttat caagggtttt ataatcttct gccatgaggg gattccttct ttcttataat
     1561 atgggagcct gacggctccg caggccgctc aaagagaccg tttttcgttc gtagttcatc
     1621 gttcatcgcg atggcttggt atttttaact cccatacgat gaacgaggaa cgacctacga
     1681 ccaacgaaaa aaatttattt tcatgtgcta gtcgcattta tttgccgatt gaatagtgcc
     1741 ctacgaacat ggtgccaaga ccctcttcgg tgagcatttc gatgaggatg gcatggggaa
     1801 ccgtgccgtt gatgatgaac acgcgcttga cgccggcctt gatggcatcg gtgcagcact
     1861 ggactttagg aatcatgccg ccggcgatga ttccgtcgtt ttccagctgc cgcgcctggt
     1921 cgagggtgat ttccgtgagc aatgaatcgg gctcgtcctt atctttgagg acgccgtcaa
     1981 tattggtcat ggacagcata cattctgcat tgagggcacc ggcaatcttg gcggctgccg
     2041 tgtcggcgtt gatgttatag gcctggccgt tggtgtcggt cccaatggtc gaaatgaccg
     2101 ggatatagcc tttatccagg ttatctttga tgatggaaac gtcgacgtcg gtaatgcggc
     2161 cgacgtagcc cagttcatcg ctctgtttct ggactttgat catctggtca tcgatgccgc
     2221 agaggccgat ggctttgccg ccgatggtcc cgatgaggtt gaccaggtct ttgttgacct
     2281 gaccggccag gaccatctgg acgactttca tcgtttctgc gtcggtgacg cgcaggccgt
     2341 tgaggaattt cgattccatg cccaatttat tgagcgtctc tttgatgtgc gggccgccgc
     2401 cgtggacgag gacgaccttg acgccgatga ggttcaagag gacgatatcg cccatgaccg
     2461 attttctcag gtcttcgttg atcatggcat tgccgccgta tttgacgacg acgattttgt
     2521 tgctgtattt cttgatgtac ggcagggcgt tcatcaagat ctgcgccttc tgggcattgg
     2581 ttacggattt gacatcttcc gagacgagtt cgattcgttt aaacattaca atagattccc
     2641 ccattaactg caggccttca aggaattttg gaagcctaat tttttcaata actgttccag
     2701 tccatcagcc agtacctcct gactgcacgg gctgctgccg gccatgccgt tggccatgac
     2761 gatgaacgta aagttttccg tgtcgatggc cgccttggcc tgagccaggg cgccttcgat
     2821 gacagcactg ccggcctggg catcggtcat gatgacacat aagccgtcgc tgtcggccgt
     2881 gccgtcggcg gcgacaccgc tcagagaaca gccgggcgtc ccactggaca aggctacctg
     2941 ataggccgtg acgtcgccgg cattatccag aaccgcttcc agcggctggc tgtcggtccc
     3001 caatcctgcc gtcagcgtct tgaggctgtg gatgagcagg gacgggcgga aatactggcc
     3061 tttggccccg ctggcgacca gggcgatatc gtctggcgct tcatccatgc atttcccggc
     3121 agcctggcag acttcctgag aaatctcatg gccccgcggc ccatcaggaa gggccgtacc
     3181 gttgtgcagg agcagtacct taccggactg agtcatcagg ccctgactcg tggcggtaac
     3241 gatggagcag tcgcgtcggc ttacgatcat ggcgacatcc aatttcacac ggctgctgca
     3301 taatccactg tacgtaccgg cagcctgaaa tccttgcggt acgctgaggc tatcataaac
     3361 ctgactcata ctgactccac ctttcatctg attcatctaa tactttaaat tcagccccgt
     3421 cgtgggatca atacccatca tgaggttcat attctggata gccgcaccgg aagcgccttt
     3481 gccgaggttg tcgaacaagg acgtgaccat gatgcgctgg tcattgccgg tgaccaggat
     3541 ttccatatcg tcgtagccgc tcatatcatt ggctgcgatg aagccgccct tgtcttcgcc
     3601 aaaaggaagg acgtggacga aggggcagcc gtcgtaatgg ctggccaaaa cctgccagac
     3661 gtcgtgagcc gtcacctttt tcgccagcaa gtggctgaag aaaggaatca tgacttccat
     3721 gccactgtag tagtcgtcga cgataggcgt aaagaccggt tcctgcgtca aatggcagat
     3781 ggtgacgatt tccggcaggt gcttgtgctg ctgtaccagg ccgtactggc gcggtgcata
     3841 taaatcctgc cctttgtcgc cttcgtactg ggcaatcatc ttcttgccgc cgccgctgta
     3901 cccggtcagg gaaaaagccg tcaccggata atcggccgga acgatgccgg cttctgtcag
     3961 gggctgtaaa atggaaatgg cgccgctggc gtggcaaccc ggattgccga cgcggtcgct
     4021 cttggcaatg gctgcggcct gctggggcga taattcgggg aaaccataga tccagcccgg
     4081 tgctacccgc cgcccggtcg acgtgtcgat gaggcggaca tcacaacctt cagcctgggt
     4141 tacggcttcg gctgcggcac cgtcagggag acaaaggaag gcgaggtcgg cctgtttcat
     4201 gcaggcccgg atggcgtcgg gatccttgcg aacatcgtc
//
LOCUS       DAWCYS010000132         4207 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1154_length_4207_cov_5.900289, whole genome shotgun sequence.
ACCESSION   DAWCYS010000132 DAWCYS010000000
VERSION     DAWCYS010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_4207_cov_5.900289"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1279 a          961 c         1110 g          857 t
ORIGIN      
        1 ccttgctgat agctaagcag acaatagaaa gtcgagaaca acgcatccat tcattagagg
       61 cggagacgga acgcatgaag ccgaaagaaa tattcgccga cgccgtcagc gcttccaact
      121 cttccatctt gattggtgac ttagcaaaaa tcttgcacgg caacggcatc aagataggcc
      181 gcggacgctt attcgcatgg atgcgcgaac acggatttct catcaaacag aaaggtacga
      241 gttacaacat gccgacacag agagcaatgt aattaggatt gtttcgggta aaggaaggct
      301 cttatgttga tggaaaaggg aacaacatca ttaccaaaac gacaaaggtt actggaaaag
      361 gtcagcagta tttcatcaat aagtttctgg cagaaaagag ctgatagcca tgaaactatg
      421 ttacagctcc gaagagttac aggaactttt ccattgctcc cggcagacca tctgccgcat
      481 ggaaagcgac ggcaggctga aacgcctgta cggactcccc ggcacgttct accgggcggc
      541 ggacgtgctg gcactttgtg aatacgaaga gccagcgcac gggccgctgg aatgggaaaa
      601 gcttgaagac gaaaacaagg ccctgtccga agagaacaga gccttgaaag aaaagttgag
      661 ccgtattaga gaggtggtgg aaatatgaaa gcgattgaga ttagggaaca tcgaccagaa
      721 ggtaaacggc gcgatcgcaa gcttttcaag cggattgcca gtgccgttgc ctttacactg
      781 gcccttgcca cggcttgtgg cattggctgg tacgcaggcg cgaaactggc ctatgaacag
      841 aaagccagtg aaattcaaaa aatccattat gtcgaagaag gggaaaccct ttgggacata
      901 gccgccgggc tgtccagtga taaccaagac attcgcaagg tcatttacga tcttgcgaag
      961 ctgaacaaca tcgcgccgga cgctgacctc aaagtaggtc aaaagattgt aatagcaaag
     1021 tagctctccc gacggttacg agagagctac agaggctaaa tgcttggata tttagttgat
     1081 tgccttaatt ataaggcaga aagtcgagga agtcaatgaa agcaagagac attaatgatt
     1141 ggggccgtaa accttggcca tttgaaacgc attacgaatt gctaagtccc ctttcacgca
     1201 gtgtatccca cgaactggca gatacctgca tgcggattgg gacgctatta aaggaaaagt
     1261 ttcccgacaa cgacgacatt acctatgaag ccgacgcgct tatttcagcc gcggaaacgt
     1321 ttgaaggcaa ctgctgtttc ccggaactga cggacgaatt gtctgacaac ttaaagttcc
     1381 ttgacgtcca tatcgacaat ttaaagcaaa ttgatgaggt tgatttatcc ggctttatcg
     1441 acgatttaac agaagtattg caggacggta aagacgccgt tttgtttgac cgtagtcgtt
     1501 ggttcaacga cacgaagaaa gcagcataac catggcaagc ttatatgaaa tcaacggcgc
     1561 aattctgaat tgcgtagaag tagaaccggg tacgacggta aatatggaaa ccggggaagt
     1621 catcgacctg gaaaaacttt ccctgctgaa aatggaacgt agcgaaaaaa tccgaaacat
     1681 tgccttgtgg gtcaagaatc tgaaagccga cgcgaaggca ctctaggtag acaacgcagc
     1741 cttcgaaaaa cggcagaagg cggcagaaaa caaggccgca cagctggaaa gctaccttgc
     1801 caacgtcctc gacggtgaaa aggtcaaggg aacggaattc tccatcggct ggcggaagag
     1861 caaggccgtc accatcaccg atgaaaagaa gctgccggaa gccttcttga ttgcacagcc
     1921 gccgaagatt gacaagacgg caatcggtaa atccttgaaa ggcgggaaag acgttccggg
     1981 tgctgaactg gaagaacgag taaacattca gatcaagtaa tggtttccgt ccccttgcgg
     2041 ggattatggt tcttatctga actggaagaa cgagtaaaca ttgcgaggtg taaaccatgg
     2101 aagaaaaaac aaatatttat aaaaaactca tggctgtgca ggctgagctg aaagcgccga
     2161 aaggccagta caacaagttt ggcgggtata actaccgctc atgtgaagac attctggaag
     2221 cggtaaagcc tttgctaagc cagcatgggc tggtgctgac catcgcggat gaaatcaaaa
     2281 ccgttggcga ccgcttttat gtacaggcag aagcccgatt aattgacgcc gacaacggcg
     2341 acgaaatcaa gaccagcgct tatgcccgcg aggcactggc ccgcaagggt atggacgaca
     2401 gccaggtaac tggcagcgcg tccagctacg cccgcaagta cgccttgaat ggcctgttct
     2461 gtatcgacga taacaaggac gccgatacat ggaataatgc acctcagagc cgttctaacg
     2521 cgcagaacaa tagccccaag ggtaattaca ctaaggcagg gcgacaggcc gaaaaaacag
     2581 cgtctagctc gtctcaggcg caaaaaaagc ctacatctgt tgatgactat taccagctcg
     2641 tcattgactg ggctagagca aatgaggccg tcatgttcat cgggccgttg ctcaaagcaa
     2701 aattcaataa ggggcatttc aaggaattgt cccttgcaga ggccaaggcc ttctacgaaa
     2761 acatcggcaa attagtcgat gcggcgaagg ccaaagacga cgccgcactg atggaaggcg
     2821 tatagcatga aattccacgt gaaaggggtc caggtgctga aaggggacag cggctatatg
     2881 ctgctgattc ccgcgccgat caccgaagac ttaaataaca tcaaagtcga cggtgattac
     2941 tccatcgaga ttaagcgcta ctcagaaaag cgctcattaa atgccagttc ttactgctgg
     3001 ctgttgtgcc agcggatagc cgaaaagctg tccagcgacg gccaatatgc cagcaaagag
     3061 gaggtgtatc gcggtgcaat ccaggattcc caaggattca cgccgatatg cgtccagcag
     3121 aaattagcag ccagcgtgtg ccgggactgg cgacacaacg gtatcggatg gattgccatt
     3181 gataccggga ccagcaaggt caagggctgt acggtccttc atctttacgc cgggtccagc
     3241 gtgtatgaca cgcacgacat gagcagattg attgactgcc tggtagatga agccagccag
     3301 atcggcgcga acgtcgagga ccgggaatgg gtccaggcac tcattgatga ctggaggcca
     3361 gaacgagatg aataaacgga aacgccgcga cgacgcgcta taccgaaaga accgccttcc
     3421 ggcctatgaa cgggctggcg gcttgtgtga aaactgcggc gcaccggctg cggaaatcca
     3481 tcacattaca ttccgttcac actgtgggac gagcgattta gataacctca tcgtactttg
     3541 tcgggactgc cacgaacggg cacacgggcc gcaagctagg cagatgagag aaaggtttca
     3601 acagattaga gggagagatt aacatggaat ttacaggtat tttcatcccc gtaaaatacc
     3661 tagacgctga tgatttgacc ttacgagaaa aagcgtttct aggttatgtc ggtatcgagg
     3721 ataacattca gatcgccgcg cttgcggaat ggctattaga cacagataaa gccgaagtcc
     3781 taaagataat ggacgacctg gaaaataaag gctatatcga agcaaggcca agtcccaaca
     3841 tgagcaccgg atggtacagg ataaggagct tgaaatgagc gaaagatata aattagggga
     3901 tatagacaac gaacaattct atcaactccc taaagcatta ttcacgaacc cgttttacaa
     3961 gaatctgccg atgagtgcaa aggtaatgta tgcacttctc cgcgacaggt tcaacctatc
     4021 caggaacaac ggatgggtag acgagaacga cgacattttc ttcatcttcg cacaggaaga
     4081 gttgcaagaa gcattgaata tttcacatcc cacatgcgta aaagctatga agcaactcgt
     4141 tgaagatgag ttacttgata ttgtgcggca aggactcaat aagcgagagc agcgtttcat
     4201 catgaag
//
LOCUS       DAWCYS010000133         4202 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1156_length_4202_cov_6.813118, whole genome shotgun sequence.
ACCESSION   DAWCYS010000133 DAWCYS010000000
VERSION     DAWCYS010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_4202_cov_6.813118"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          953 a         1221 c         1035 g          993 t
ORIGIN      
        1 gagttttaag tttgtagttt ttagtttgca gaaaatggta aaaaatttaa aaccaacttc
       61 tacaaactat aaagtaaaag gaacctcgca tgatggtaga agccttcgtg caccagccct
      121 tcttcttact cgtgaatcgt cattttaccg atggccatgg caatatgcgg gtccatttcg
      181 tcatagatgg tactcttgcc cgtatccagc atgcggatgg ccttcatgtc ttcctcatcg
      241 agggtaaagt cgaagacatc gatgttttcc tgcatccgcg ctttatggac cgtcttgggg
      301 atgacgacga tatcccgctg gagcagccag cgcagcatga cctgaccgac ggtcttgccg
      361 tgtttgtcag cgatgtccct caaaagctgc gtatggaaga catcgttgtt cccttcagca
      421 aagggcgccc atgcttcctg ctggatgccc agttctttca tgaggccttc ttcagcccat
      481 ttctggtccc atacgttggt ttcaatctga ttgacagccg gcgtgatggt attcatgttg
      541 gccaggtctg ccagccgttc gttccagaaa ctggtaacgc cgatggcccg gatacggcct
      601 tcctgataga gccgttccat ggcccgccag gccccatagt aatcgccata gggcttgtgg
      661 atcaaataga gatccaaata atccaggcct aattttttca tcgacacgtc gaaggccttc
      721 agggtcgcat catagccatg gtcctggacc cataatttcg tcgtgatgaa taactcttcc
      781 cggcggatgc cgctctgacg cacggcatca ccgacacctt cttcatttct gtaagacgca
      841 gccgtatcga tgaggcggta tcccgtttcc aaggcttcac tgacggcttt ttccgtttca
      901 gccgatgccg tctgatagac accaaatcct aatttcggca tgatgacgcc attattcaag
      961 gttacaaatt ccatgatttc ctcctatttc tcttcaaagg gcagatgcat attggcatag
     1021 cgttcttcta aatccggtgt tgccgtgtag tactggcgcg tcccgttcag cccgttgaca
     1081 acggccattt cttcatcagt cagggcaaaa tcgaagatgt cgaaattgct gcgcagatgg
     1141 tccggattct tcgtgccggg gatggtgatg aactgccgct ggacagccca gcgcaggata
     1201 atctgtgccg tactcttgtg atacttgtcg gccaatgccg taaagattgg gtcgcggagc
     1261 agttccgggt cgccatgccc caatggatac cagcccatga gcttcgtacc gtattcagcc
     1321 aggcgggcca tgatatcatc gtagacgtgg tacgggtgcg tttccagctg gatgacatgg
     1381 ggcttgacat cggcatgggc caggagtttt tccagtttgt cgccatggaa attggaaatc
     1441 ccccgggaac gggccttgcc gtcgcgatag gcctgttcga tagccgcata cccggccaga
     1501 taattgcctg ccggctgatg gatgaacagc agatcgacat agtcgacgcc cagccgttcc
     1561 agggtcttat cgacagctcc tgccttttca tacagcgtcg gccacaactt cgtcgaaatg
     1621 aaaatatctt cccgtttgac cagaccttct tccaaagccc ggcgcaatcc cctgccgaca
     1681 gcttcttcgt tgacgtaggc attggccgtg tcgatgaggc ggtaacccat cttcaaggca
     1741 gcataaacgg atttctccgt atcttccggg ccgatcatga aggtccccag gcctaacaac
     1801 gggacctgca cgtcgttgct caatgtcgta tattccatgg tataatctcc tttcagactc
     1861 tgtttatatg atatagtgtg taataggtat ttcctgtcta catctcgcat tttacaacag
     1921 ctttcaaaag atgtacaatg ccaacagggg aattcactat ccacttttta tatactgaag
     1981 cagagaaagg agccactatg atagaaacga acctgcttgc acaatttgtc gccttcgcca
     2041 aggccggcac cctgacccgg gcagcccagg acctgcacac gtctcagccg gcactgacgc
     2101 gctctatgaa aaaactcgaa gaagagctgg aggtcccctt gttcgtgcgc cacaaaaacc
     2161 acctggaact gacacagacc ggccgttatg ctgtctatta cgcccaacgc gtcctccagg
     2221 aaaaccagga tttctgcgac cgcatccgca gctatgaccg cagcctgcat accctctcca
     2281 tcggctactg tgcaccggtc ccgcaaatgg tgctgacgcc cttgctgaac gggattttcc
     2341 agggcatgac cttatcggcc gacatgaagg acgatgtctc cttcccggaa ggcctgcgcc
     2401 gccagaccta tcagctggcc gtcatgcatg cccggccaga cggcgacgag ttcttcgtca
     2461 agaaatgcgg cagcgaaaaa ctttatctct cggtccgcgc atcggacccc ctggcctttt
     2521 atccggaagt ccgcctgcag gacctggatg gccgggccat cctgctcttt tctcatatcg
     2581 gcttctggat gaaaatgacc cgggaaaaga caccgcacgc ccgctatttg ctgcaagtcc
     2641 agcgggatgc attccatgag ctgacggagc actcggacta cccctgcttt tcgtccagct
     2701 atttcctcgg acagggacaa aaaatcccgg gccgcatcaa catctccctg gcggacccgg
     2761 aatgtcagac agactattac ctagtctgcc tgaaaagtga acagaaacgt tatgaaaaac
     2821 tcttccagca aatcgacgac cggactatct actgatggga ttccaggtcc cggcgcagtt
     2881 tatccaggac ctgggccgtc aaatcgtgcc aggcatcctg gagccactgg tcccgctgcg
     2941 cttcgtcgcc gatacattcc agggccttgg ggatggcctc gccgaagagg atatcttccg
     3001 tctgacggct gcggtagacg ccgagcttac aggcattggc aaagtgttca cagttatgga
     3061 agagcaggtc gtatttttta ttgcccaggc aggcacgggc aaaggcaatc gtctcgggta
     3121 cagaacagag ctgtgcctgt tcttccggcg tatactgacg cacttctacg tcaccgccct
     3181 ggcggaactc atcgaacgac gtcgaccgga tttccgtatc ccaccagtcg atgggattgt
     3241 agtcgtcatc tccggcaaag gcgatgactt catcgtcact gacatagatg ccgtgatggt
     3301 aatacaagcc tcccagccgg cgcacgcgga tatggtcgcc cataatcggt gcctgccggg
     3361 cccaatgttc cgacgccccg tcgtcctgca ataaggataa aatcgaattc aaatcaatgg
     3421 ccatcgtcgt cactccttac aaaattgcct aactcttacc aggctgtcac cattctttta
     3481 cataatgccg ttatagtaat aactgtaaac gaagtcagct atccccggcg gcaacccacc
     3541 ggcccgttac cgaacccctc tgccaggatg gcctcccgac cagaatcgga gaggttcgcc
     3601 gtactgtcca ccggccagcg tgccgctaaa cttggtatca tagaatcaga aaaaaccccg
     3661 gagacctccc gctccggggc tttttctata ccggcagccc ctacaggaag gtcagcagtc
     3721 cctgacagcc ctcctggaca tcgtcccagg tccgctggaa atcccgcgta taccacgggt
     3781 cggctacagg acggggattt tccgtataat ccatgagaag atggcacttc tggtccgggt
     3841 cgccatggaa gatgcgcttc atgtcccgca ggttctcgtc atccatgccg atgaaataat
     3901 cgtaaactgc ataatcgcca gccatcagca gcacggcccg cttatcactg gggtccatac
     3961 cgtgtgcccg gagcagccgt tccactggcg gatagaccgg attgccatag ccgttccaga
     4021 tttcctcatc cgtcgtagcc gccgatgcca cgtcgaaccg ttctgccagc ccggcctgct
     4081 cggccatctg ccggaacaca aactccgcca tgggcgaccg gcagatattg ccatggcaga
     4141 taaatagtat cttgatcata aaggtgaaac tccttccata ctgcaaatta cctgtagaaa
     4201 ac
//
LOCUS       DAWCYS010000134         4196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1159_length_4196_cov_6.363439, whole genome shotgun sequence.
ACCESSION   DAWCYS010000134 DAWCYS010000000
VERSION     DAWCYS010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_4196_cov_6.363439"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1021 a         1170 c         1066 g          939 t
ORIGIN      
        1 gcttaactat acgaaaggat gtctttgttt tgttattata tatcattaaa tggctttatg
       61 cctggatcct gcctatgggt ggcatcgtcc tggctttgat tctcctgttg tgttacatgt
      121 tccgcaagca cagtcccggc cgctgggctt tggctgctat tacggctgtc ttttatctgc
      181 tctctatcga acccgtgtcg aatggcctca tccatccttt ggaaacggcc tatgaccagc
      241 cccagggggc agatgtagac ggcgacgtca tcatcctcct cggcggtggt tctcgtgccg
      301 gcgttcctga tttcgatgga accggtcagg tcggcagtgc ggcggccaac cgcttcctta
      361 tggccctgcg cctgcaaaag gcgaaggatc tgcccatcct cttatcaggc gggaccatcc
      421 tcaagggcga cgccaatgaa tcggaaatcg aaaagcggat gctcttatcc ctgggcgtgc
      481 cggaaaataa gattttcatg gatgacaaga gccgcaatac ggcagaaaat gccgcttttt
      541 ccaggcaaat ctgcgagcac cagggctgga agaagcccat cctggtcacc agcgccttcc
      601 atatgccccg gtcggtccgc ttctttacgc gccaaggcct ggacgtgacg ccctatccct
      661 gtgattacca cacgagtgca gcgacgaact ggtcgggcta ttccatcgtg ccccagtctt
      721 tttacttatt caactcctgt ctggccatca aggaatatgt cggcataggc gccgcttctt
      781 tgcggatgca ataaattggg acataaagcg cccatggtat tgcaaaatcg tacccgatgt
      841 ggcatactta cagtgtaagt agtaaggccc tcaagttctt agaattatgt aaaagagtta
      901 ttttcgtttt caaaaggagt gtatcgacaa tgattaaagt tggtattaat ggtttcggtc
      961 gtattggtcg ttttgttttc cgcgctgcac agactcgtga tgacatcgaa atcgtaggca
     1021 tcaacgacct ctgcccggtt gactacatgg catacatgct caaatatgat accatgcacg
     1081 gccagttcga tggcacgatc gaagctgccg tcgaaaacag ccagctcatc gttaacggca
     1141 aataaatccg cgtaacggcc gaaaaagatc cggcaaacct caaatgggac gaagtcggtg
     1201 ctgaatacgt cgttgaatcg acaggccgtt tcctcaccat ggacctcgct gaaaaacacc
     1261 tcgcagccgg tgcaaaatac gttgtactct ccgctccttc caaacagggt gctgacggcc
     1321 gccaggcaga catgttcgtt tgcggcgtta acaccgatac atacaaaggc cagaaaatcg
     1381 tttccaacgc atcttgcacg acgaactgcc tcgctccgat cgctaaagtc ctcaacgatg
     1441 ctttcggcat caaagaaggc ctcatgacga cggttcactc cacgacggct acgcagaaaa
     1501 ccgttgacgg cccgtccatg aaagactggc gcggcggccg tgcagctgct ggcaacatca
     1561 ttccgtccac gacgggtgct gctaaagctg tcggcaaagt cattccggaa ttgaacggca
     1621 aattgaccgg tatgtccatg cgtgtaccga ccctcgacgt ttccgtcgtc gacctcacgg
     1681 tcaacctcgc taaaccggct acgtacgacg aaatctgcgc agctatgaaa aaagcttccg
     1741 aaggcgaact caaaggcatc ctcggctaca ctgacgaagc tgttgtttcc tctgacttcc
     1801 tcggctgccc gctcacgtcc atctttgacg ctaaagctgg tatcgctttg acggacacct
     1861 tcgtaaaagt cgtttcctgg tacgacaatg aaatcggcta ctccaacaaa gtcctcaacc
     1921 tcattgctgt catgaacgac tacaacagca aacactaatt tgtagcgacg catttcccga
     1981 cgggtaaaca gactcccgtt acaaatcaga ttgaacccta aaggggccgt cgcttgaagg
     2041 ttctcgttct catgcacggc tcctttttct ttcaaggttt ttagtgtaaa gtttgtagtt
     2101 tgtagaaagg gcttgaattt ttagccaatc actgcaaact acaaactgca aacttcaaac
     2161 tcgaaaagag gctgtcttaa tgggacagcc tctttttact ttctcacatc acaaaggagt
     2221 caaatactgt aatgacgaaa aagaaacctg taatgctggt catcatggat ggttttggct
     2281 gcagcagcat cgctaaagat aacgccgtag cccaggcaga cctcaaggtc ctgccgaaac
     2341 tttggaaaga atatcctcat tcgtatctcg gcgcatcggg cgaagacgtc ggtctgcctg
     2401 acggccagat tggcaactct gaagtgggcc atcttaatat cggtgcggga cgtattgtgt
     2461 accaggcgct gaccaagatc accaaggata tcaaagacgg agctttcttc aagaagccgg
     2521 ctctcgtcgg tgccatggaa aacgtcaagg aaaaagactc ggccctccac ctgctgggcc
     2581 tggtatcgcc tggcggcgtc cacagcagtg aacagcatct ctatggtctt ttggaaatgg
     2641 cgaagcgcta tggcttgaaa aaagtctaca tccacgcctt cctcgacggc cgcgacgtac
     2701 tgccccgcag tgccggcgaa tacctcaagg aactggaaga caagtgcgct gaactcggcg
     2761 tcggtgaaat cgccaccatc agcggccgct actatgccat ggaccgcgac aagcgctggg
     2821 aacgcgtcga aaaagcctat gatgccgtcg ccctcggcga aggaccgcag gaaccggatg
     2881 cccagacctg cctggaacat tcctacgcag ctgacgtttc cgacgaattc gtcatcccga
     2941 cagtcatcca taaacatccc gtccaggatg gggacagcgt cgtcttcttc aacttccgcc
     3001 ccgaccgggc ccgtcagctg acgacggctt tcgtcgtccc cgatttcgac ggcttcccgc
     3061 gtccgaagaa actggacacg tactttgcga ccatgacccg ttacgaagac gaccttccgg
     3121 tacacgtcgt ctatgataaa gaacggatcc ccgacacact gggcgaagtc ctggccaagg
     3181 ccggcaagac ccagctgcgc attgccgaaa cggaaaaata cgctcacgtc acgtatttct
     3241 tcaatggcgg tgaagaagaa cccaatcccg gtgaagaccg catcctcgtg ccgtcgccga
     3301 aagttgcgac ttacgattta cagccggaaa tgaacgcgcc tatcgtgacg gaaaaagtca
     3361 tcgaacagat caaggccgat aaatataaca tgatcatgct caatttcgcc aatgccgata
     3421 tggtcggcca tacgggcgtc tttgaagccg ctgtcaaggc cgttgaaacg gtcgatacct
     3481 gcgtcggcaa gattgtcgaa gccttgaagc ccgtccaggg ccagctcctg atcatcgccg
     3541 accatggcaa tgcggaacag atggccgatc ccgatacggg atgcccttac acggctcata
     3601 cgaccaatca tgtaccgtgc atcctggtca gtgaagaaca taaaggggaa cacctgcacg
     3661 acggcatcct ggccgacgtc gccccgacta tgctgaccct ggccggtatg gccattcctg
     3721 ccgatatgac cggtaaatgc ctgctggatc agtgattata ctctgttgtt ctatcgcatc
     3781 gtgaagggag agagtaagat ggaacaagat acattgagta tctttcaaaa aatagctccc
     3841 gatgtcatgg ggacaatcaa agaacgttac ctgctgctgc ggcatatcgc cgacgccgaa
     3901 cccgtcggcc gccggaccct ggccggcctt tcgggcctgt cggaacgcgt cgtccggtcg
     3961 cacgtcgacg tcctgcggcg cagcggtatc atccgcttta cgacgctggg cattgctatg
     4021 gagccggaag gcaaacggct catgccggat ctgttggaat gtttcctgcg cctgaactat
     4081 ttggatgaca tgcagcgcca gatgcgccag aagctggacc tgaagaatgt ctacatcgtt
     4141 cgcggtgaca gtgacagcga taagacagcg aaggaagaac tgggccgccg tggcgc
//
LOCUS       DAWCYS010000135         4177 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1163_length_4177_cov_5.142649, whole genome shotgun sequence.
ACCESSION   DAWCYS010000135 DAWCYS010000000
VERSION     DAWCYS010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_4177_cov_5.142649"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          987 a         1229 c         1130 g          831 t
ORIGIN      
        1 gatatggaat ggggcgtcga cgaacggaac aataacgtat ggctcctcca ggcccgtccg
       61 gaaacggtat ggtccaagaa gaataaagaa aagaaagaag ccgctgccga aaaggccgct
      121 gtcacgacgg accgcaaagt cgtcgtcaaa ggcttgccgg ccagcccggg cagcgctgct
      181 ggcaaagccc atgtcatcct cgacccgtcg aagatcgctg aattcaaaga cggtgaaatc
      241 ctggttacga ccatgaccgc tccggactgg gtaccggcca tgaagaaggc caaggccatc
      301 gtcaccgatg ccggcggcat gacctgccac gcttccatcg tcagccgtga attgggcatc
      361 ccctgcatcg tcggcacgaa gagccgcagc gtcgaagcgc cgacaaccct ccaggatggc
      421 gcagaaatca cagtcgatgc gacgaacggc atcgtctatg aaggcatcgt cgccgacatc
      481 gtcaaaccgg aagctgctga agccccggcc ggccagactg caacgaccgt cgtcgctgcc
      541 gaagccgctc cgattacggg cacgaagatt tacatgaacc tgggcaaccc ggatttggct
      601 gaaaaatacg gcaaactgcc ttgcgacggc atcggcctca tgcgcgaaga attcatctgg
      661 acgacattca ttcatgaaca cccgttatac ctcatcgaaa cgggccgggc tgaccgcgtc
      721 gtcgatcagt tgtccgaagg tatccgcaaa gtctgccagg cactggctcc gcgtccggtc
      781 acgctgcgcc tgagcgactt caaatcgagt gaataccgca acctgacagg cggtgacaaa
      841 tacgaacccc aggaaagcag cgccctcctc ggctggcgcg gtgcttcccg ttactatgat
      901 ccgaaatacg tcgaagcctt caaactggaa ctgaaagctg tccgcaaagt ccgcgaagaa
      961 tacggctaca agaacctcaa cgtcatgatc cccttctgcc gccgcgtcga tgaaatgcag
     1021 aaagtcgtcg acctcatggc tcaggaaggc ctccaccgcg gtccggactt caaggtctgg
     1081 ctcatggctg aaatcccgtc caacatcatt ctggctgacc agttcaacca gttcgtcgat
     1141 ggctattcca tcggctccaa cgacctgacc atgctcatca tgggctgcga ccgcgacaac
     1201 gatacggtag caccgctctt cgatgaacgc aacctggcag tcaaacgggc tatccgccat
     1261 ctcattgccg tagcccataa ggacggcaag accgtttcca tctgcggcca ggcaccgagc
     1321 gtctatccgg acttcgctga attcctggtc aagagcggca tcgattccat ctcggtcaac
     1381 cccgatgccg tcgtatcgac acggaaacac gtggcacaag tcgaacagcg tatcctcctc
     1441 gattcgctga caggcaaggg ccgtgctgat acagaagatt atacgtggta atagcatatc
     1501 cgatttatga gtaatagaaa ggcggtccca aaagaaaaac agacttttgg gccgcctttt
     1561 tctcatacta ggaccttgta ttatacacta tttgcatatt atacatctta tatcgcggga
     1621 tgtttttata tatgttatga taataagtga ataagagaac gcttgctatg tagggggtga
     1681 aaagacgcta tgttatcgga acgtcaggaa acgattttac agattgtaaa ggaaaacagt
     1741 ccgattacgg gccagcatat tgccaaccgg ctccacgtga cgcgggcagc cctgcgctcg
     1801 gacctggcca tcctgaccat gttagggctc atcgatgcgc ggccgaaatt aggctacttt
     1861 tttacgggca ataaagaaaa agacctgctc tcgcgggcca tcacggatat caccattgat
     1921 tcctgcctgt cgcagcctgt gatcgtcggt gagcatacga gtgcttacga tgcgatcatc
     1981 gccatgttta cggaagatgc ggggaccgtc tttgtcggga cggacaatat cctggccggc
     2041 gtcgtatcgc gaaaggattt attgaaaacg gctatgggca gcaatgacct gcgggccctg
     2101 ccggtgcgca tggtcatgac gccagtgagc aaagtgatct atgcccagcc tgaggaaaag
     2161 gctatcgtcg ccgctcagcg cctgatcgaa tatgaaatcg actgcctgcc tgtcgtcgag
     2221 gtcgaaagta ccgatgaaga cgacaagaag gaattgaaga tccttggcag gatatcaaaa
     2281 acgaacataa cccgtctctt tgtagaatgt gggttaggga ggagataact gtggaaaaac
     2341 cgattattta tgcctgctca gactcgcttg gtgaaacggc tgaacgcgtc gcccgggcag
     2401 cagccagccc agtacgacaa gcaagaattc aacatcatcc gcgtaccgta catccggtcg
     2461 gaacagcaaa tcgaggagct ggtctgcaag gccgaagctc acaatgccat gatctgctac
     2521 accatcattt cgccgaccct gcgccggaaa ctgtgggaac tgactaagtc ggccaacgtc
     2581 accgtcgtcg atatcatcgg ccccatgctg gccggcgtcg gcaccctgac ggatacggaa
     2641 ccgactttga aggccggcat gatccatcag ctcgacgaag aatatttcaa acgcgtcgaa
     2701 gctatcgaat tcgccgtcaa atacgacgac ggcaagaacc ccatgggctt cttgaaggcc
     2761 gatatcgtca tcgtcggcgt ctcgcgcacg tcgaagacgc ccctttccat gtacctggcc
     2821 cataagcgca tcaaagcggc caacctgccg ctggtgccgg aagtcagcct gccgccggaa
     2881 ctgttccaga tcccggcccg caagatcgtc ggcctcatca tcgacccgtt caagctgaat
     2941 ttcatccgtt cggaacggct gcgcagcatg gggctcgatg ccaacgccag ctacgccaat
     3001 atcgaacgca tcaatgaaga actggaatat gcccgcggtg tcatgcgccg cctccattgt
     3061 cccatcatcg atgtgtcgtc caaggctatc gaagaaacgg cgaacctggt catggaaatc
     3121 gtcaaacgca ataaagaact gtatccagaa taaaatcagc caatggcggc gaggggggct
     3181 tgttgcatca tggttcttac cattatgcaa gggccctttt tgcgtcactg cgtgaggttt
     3241 gacattacga catcaaactt cggaaggagc actatgaaag attcgacgac agccattttc
     3301 acgtccctgt cggcggccct ggagagccgg cattttttgg cgacgctggg aatggaaaaa
     3361 gatgaaatga cggcctggat acgccgcagc cgcctgcggt ccctggccga tgaattacag
     3421 cactgccgcg acggggaagg gcgcttttct tcgcctgctg tcgtcgcggc ggccaaaccc
     3481 tatctggact gcctccggga cgaaccggcc ggcggctggc tgcgctgctg ccatcagcgc
     3541 gtcctggccc gcctctttcc cggttcctgc cagggcccgg aggccatggc cctgacaggg
     3601 cgcggcagtc atggcgacat ctaccggcgg ggctgcctga tcctcatgca ggtcctgtgc
     3661 ggcctctacc gctatgaacg ccaggtcctg cccttcgacc ccaagcggga catgcccctg
     3721 gtatcggtcc aggaaatcat ggcccacggc tacaccaagg aatacctgcg cctgcgtaca
     3781 ctggctgccg acgattatat ttatgaattc atgcgcatcg gcgtcgacat tacgccttac
     3841 aatacgctgg gccacgtcgg cggcgtccac tacgtcgccg tttacgcagc tcgccagctg
     3901 gcgcggaacg gcatccccgt cgacgtcgcc ctcatttccg gcgccgctgc ctgccatgat
     3961 atcggcaagt acggctgcaa gaagaatgaa gagaagcggg tcccttattt acattattat
     4021 tatacgggca tgtgctgccg ccgcctgggc ctgccgggta tcggccccat cgcggctacc
     4081 ccttccgtct cggcccgggc actggaaaac ctgtccgtcg aatcgctgtt gctcatatag
     4141 gccgagatcc gagtcaagcg caggcgcgat gcccacg
//
LOCUS       DAWCYS010000136         4141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1174_length_4141_cov_5.850954, whole genome shotgun sequence.
ACCESSION   DAWCYS010000136 DAWCYS010000000
VERSION     DAWCYS010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1174_length_4141_cov_5.850954"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          886 a         1115 c         1249 g          891 t
ORIGIN      
        1 atgcacaaga tgacgtacgg caggttgaag acgacgtggg ccatgagaat cgaaccgtag
       61 cccaggcgca ggcccatgcc taaaaagagg agcatcaggg aaatcccggt gacgatgtcc
      121 gcattgagca gcggcatact agccacgccg agatagaccg accgctgttt gcgacccatg
      181 ccgcgcaggg ccatgcaggt gagaagaccc aggacggtgg cgatggccgc cgacgagaga
      241 ccgatggtaa tggtattttg caaggcgtca aagatgcctt cattctggaa taactcgccg
      301 taccattgca gggtaaagcc tgtccagtgg ccgcggtaac ggctggcgtt gaaggactgg
      361 gccacgagga cgccgatggg cgcgtagagg aagaggacga ccagggcgac gtagagtttc
      421 ttcacttttt ccatcacttc ttcacctgcc cttcgctgcg gtcgaaataa gccgtcacga
      481 ccatgttgac gatgatgaag accatgagca ccagagacag gccgctgccc agatgccagt
      541 cataggccat ggtgaattcc tgttcgacga tgttgccgat gagcaggatc ttattgcccc
      601 ctaagaggcc gctgatgaag aacgtcgtca gcgacgggat gaagaccatg gtgatgccgc
      661 tgatggcgcc gggcagggac aagggcagga tgaccttggt gaaggtctgc caggaattgg
      721 cccctaagtc gcgggccgcg gcgatgacgg tgccgttgat gttgctcagt gcgacgtaga
      781 ggggcaggat catataggga aggaagttgt aaatcatgcc gatgacgatg gccgccggcg
      841 tgttgatgag gcttacgtca ggcagggaca agaggcgcag gaactggttg aggatgccgt
      901 tcttttccag aatcgtctgc caggccatgg tcgtcagcag ggagttcatc cacatgggca
      961 ggacgaagag caggaacagg agcgagccct tcccccgctt cttttccgtc aggataaggc
     1021 agagcggata agccacgacc aggcagatga ccgtgctgat gagggccagg acgatggaca
     1081 gttccagggc cttcaggtac tcccagtggg cgatgaggct gatattggcc agggtaaagg
     1141 cgccgtgggc atcggtcagg ccgtaccagg cgatggccac gacgggacag aagataaata
     1201 agaccgccca gatgaggaag ggcgcggcaa agaggttgcg aatcgctgct gaattaatca
     1261 ttttccatcg ccctttcgtc ttccgattcc ggtttattca tgatctggat attgaagggg
     1321 tccacccaga ggctgacttc gtcgccgggc tgatagtgct tcgtcgattt gaccagccac
     1381 tcgatccccc gcgcctggat agtgaggtta taggtcatgc cttcaaaaat cgtcttggtg
     1441 acgacgccgt tgaaaccgcc gtcgcgaggc ggctgcagtt ccatatcttc cggccggata
     1501 tggacgtcga cagggcggtg ggtcccgaag ccgacgtcga cacagtcgaa gtcgtagtcg
     1561 gcgatgcgca cgagtttgtc gtgtaccatg aggccgtcga cgatgttgct gtcaccgatg
     1621 aagtcggcga cgaaggcgtt ttccggttca ttgtaaatgc tttcgggcgt gccgatctgc
     1681 tggatataac cctggttcat gacgacgacc gtgtcggaca tgctcagggc ttcttcctgg
     1741 tcatgagtga cgaagacgaa ggtaatgccc agttcttctt tgatcttgat gagttccagc
     1801 tgcatgctgc ggcgcaattt ccggtctagg gcactcaggg gttcgtccag gagcaggacc
     1861 cgcggctcat tgacgacggc ccgggcgatg gccacgcgct gctgctggcc gccggacaag
     1921 tcgacgatgt tggcgtgtgc gtagccgtcg aggttgacca gtttcagggc ataacggatc
     1981 ttatcgcgga tataatcgcg ggacttgccg tgaatcttcg gcccgaaggc gatgttttcg
     2041 gccacgtcca tatgggaaaa aagagaataa ttctggaaca ccgtattgac ctgacgccgg
     2101 ttcggaggca gctgggtgat atcggcccca tcgaagagga tacgcccttc atcaggcgtt
     2161 tcaaaaccgg caatgaggcg gagcgtcgtc gtcttgccgc agccgctggg gccgaggaga
     2221 gtcacgaatt cattttcaga aatcgtcaga tcaaggccat ctaatacctt gtgcccatca
     2281 taggtcttgg atacgtgctc caggtgaatc aattcttttt ccatttcaca actccttttt
     2341 tcattcctta cggctccagg ccgtaaaaaa agggagatcg cataatcgct gatgcaaggt
     2401 cccttcctcg atattatttt ttactgctcc gcgtgtccca gggccgcgac gtcgtccaat
     2461 acgacacagg ctgcgggctg cggaccgaat tggctacgcg ggccgtcttg ggccggaccg
     2521 tagtcttagt ctggacagcg gctgctttct gactgtactg ttcatgccat tctttcaggc
     2581 tcggaaaaat gggtgcttcc gtttccgtca cggtcatggc cgaccgcttg tagggcttca
     2641 gggccatggc ttcatgaccg gcaaggcgcc agatttcccg ccgcgtctgt tcgacggtct
     2701 ggacataatt catggtctcc gtataatgcg gcacgcctcg ggcccggtcg acggcgcccg
     2761 gtccggcatt atatgccgct tcggcataga cgaagggccg gtcccatgtg cggtagcgtt
     2821 cgaccatctg gccgaggtac ttgacgccgc caaagatgtt ctccctgcgg ttgtagggat
     2881 tgaccttgag tgatgccgct gtagacggca taagctgggc aatgccgacg gcaccgacgc
     2941 cggacacggc agaattatcg aaatggcttt ctgtcgtaaa gacggcggcg acgaggatcg
     3001 ggtcgacgtc atacgtatag gatgcatcac agatatcctt ggcaatggcc gccgcttcgc
     3061 tgccgcggat atatttgctg acctggtcat agaccatttc ttcctgcgta atggcctgca
     3121 ccggcatcaa ggccatgagg ccgatgaaaa gagcagccat ctttgtctta caggctttgc
     3181 tgagtatcaa ctttcacatt cctccccact cacacgacta tcattctacc tttcattata
     3241 tcacggtcgt caagccattt gccaatacct tctcgttctg ccctattagg gttatgcatc
     3301 attgttccac cacgcggaag atgacgcctt cgtcgcagtc gggacactgg atatccgacg
     3361 gccagagcga accctggcgg ccggcgaaga cataaggata aagggcctgg agggccgtca
     3421 tgcagagggc gccgctcttt gctttatcga tgacaggccc gtcgaagacg atttcatcgc
     3481 cttctttata aagatcacaa tgagacttga tgacgactaa cttaactttc ttggcatcca
     3541 taatcatacc tccttaaggt tcaatgggaa cgaaatcgaa ggcttcgacg tcgaagagac
     3601 ccgtgtccag cagtttcagc ttggggatga cgggcaggct catgaagcac aacgtcatga
     3661 tgacatcgac gtcagcgctg acacccagga tatggtgggc cttggcgtag agctcgtcca
     3721 tagctgcatc gacagcggcg gccggctggt cgctcatgag gccggcaatg ggcagcggga
     3781 tggatgcaag gaccgttccg tccaagacga tggccatgcc gcccttctgg ccctccaggg
     3841 cctcgacggc agcggccata tccctattgt tcgtgccggc gacgatgatg ttgtgtgaat
     3901 cgtgggccac ggatacggcg acggcacctc gtttaaggcc ataaccgcgc agcaggccca
     3961 ggccgacgca gcccgtgccg tgatggcgtt cgacgacggc aactttgacg atatcggcct
     4021 gcgggtcgta gacgaaatcg ccgcggccat cgcgatggat gagggcttcc cctttttccg
     4081 tgacgacgcc gccgggcacg atgtcgatag tccggacccg gtccgacgtc aagtgcataa
     4141 c
//
LOCUS       DAWCYS010000137         4120 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1184_length_4120_cov_7.286839, whole genome shotgun sequence.
ACCESSION   DAWCYS010000137 DAWCYS010000000
VERSION     DAWCYS010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1184_length_4120_cov_7.286839"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1019 a         1048 c         1100 g          953 t
ORIGIN      
        1 tcatgctacc gccccctctc gcgcccgttc ccgaccgtaa gcgtaatcgc cggacgtccc
       61 cttgaccgtg aacaagccta aataggatac agccatgatc gactgggctg ccgtcgccgc
      121 cgtatcctgt cccaggacgg gcaggacata gctggccagg taacaaatga agggaacggc
      181 aaacatgagg ccgggcttat tgatgtcgtg acagcgccgg acgcccaggg aaatctggga
      241 ccaaatagcc ggaatggaca gcagaacgaa gatgatacca aagatgatgg catattccat
      301 atgattgatt aaaatagatt cgacgatctt gctgtagagg atgatggaaa acatgaactg
      361 ggcgaacatc aagatcagcg tccggaaaat aaaaggccgg cgctggatgc ggccggtaaa
      421 gcggaaatac ttggccttca gatcggcaaa gtcgcggaaa ctgaccaggc tgttttcact
      481 gcccggaccg acaggcatgg caaagccgcg atggatacgt gccgattggc gggaccgctt
      541 cttcaattta attttttgat tgcgttgaga cacaacgatt acctcctata aaatactctt
      601 agtattatcc tactatattc atggcctcga ttcaattcat ttccggcaaa atatgctata
      661 atagaaaaaa ttacttcaag gaggtcctta tggaatctac tgtctttacg aatctgcgcg
      721 gctctgaagg agccctgacc tttaattttt tctgtgaatc cctgatcacg tcgctccaca
      781 ccttgacgca cgtcatggaa gacgccggcc ttgccgttcc ggacaacgtc ggcgacattg
      841 ccgatgcctt gggagaaatg ggcagccacc tcatggaaga ctaccagcgc ggcgagctcg
      901 acttaggccg cttcaaagat gaaatcctcg acttctacga cctcaacttc gccgtcaacg
      961 acgccctggc cttggccatc atgagccatg acgacctgca gtattactac tacgtctata
     1021 tgcagggcct gtacatcttc ttccccaaca tgatggaagc cttcaacgcc gacatcgacg
     1081 acgccaagat catccccttc ctggacgaac tggccaacga attccgccag ctcgccggca
     1141 gcggattgtg atggcgtctg tggcctagtg gctcgtggtt gcggcccgtt gtgggaatcc
     1201 cgccatcggc atccgtaggg gcgaccacgt ggggcgcccg ccatggggat cccgccaacc
     1261 tgattcaaaa aaaggggctg tcgcacgtac gacagcccct ttttagggtt agcagctagt
     1321 ggctagtggt taggggatgg ctttaaattt aaaggttttc tctgacaact acaagctccg
     1381 tactacaaac catactcgca tgatggcagg ccaacgccct acaggatgaa gaagtagacg
     1441 aaggaaatga cggctacgac gaaacggtag taggcaaagg atgccagcgt cgacttgttc
     1501 atgaatttca ggaaccagag gaccgagatg taggcgacga tgaaggccgt aatgaagccg
     1561 atgacgatca tgaccaggtc gttgccgctg aggtcgctca ggctcttcaa gaggtcatag
     1621 aaacaggcga cgatcatgac cggtacggcg atgatgaagg aaaagtcagc tgctgctttc
     1681 cggctgaggc ccaggaaaag gccgccggca atggtcgaac cggaacggga aaagccgggc
     1741 cataaggcca ggatctggaa catgcccgtc aggaaggcct gggcaatagt catcttttcg
     1801 acgtcgttac agaccgtctt ggtatgggac ttttcagcga cgagcatgaa gataccaccg
     1861 atgatgaggc cgatgatgac cgtccctgcc gaaaagagat aggtcttgat ggcgtggtgg
     1921 gccagatagc cgatgcccat gaccggcacg atgccggctg cgacgtgggc cagggtcagt
     1981 cccgtatcgg tgcggaacca gttttcccgg cggaagaggt cccagcagcg gtactgatgg
     2041 agcatgcgga tgaacttctc tttataaatg acgatgaccg acaagatagc gccgagctgg
     2101 atgaagactt caaagacgct ggcccgggga ccggtaaagc cgagcatatc gccgacgaga
     2161 atcatatggc ctgtcgatga aactggcaaa tattccgtga ccccttcgac gatacccagg
     2221 atgacggcaa taatataatc actcatgttt ttaactcctt ttcgctgaat acctgatgaa
     2281 atgtttacaa caatactaga aagtatacca caatggcagg taaaaaaaaa gtgctgccgc
     2341 gcaaagagct tttctttgcg ggacagcact cattctaaca agtatctatg aaaaaacgat
     2401 tgcgaaaaaa taacgaatct ttaaattacg cctattttta ttcgtcgagg caaatgacgt
     2461 gattcgggtt atcgatgacg aatttagcat atttcggatc gccattgtgt tcaaaataca
     2521 aagtattgcc ttccaggctg ccgcgccagc tgacgataat cgggcattcg cggctcaact
     2581 tcttcagcat cgctacgggg tcgaggttca ggatcggtgc gaacaggaat tcaacattat
     2641 caatcatgac aacacgggca ttgagacgat gaatggctgc cgacaccagg gcaattgcct
     2701 tatccgggcg gtcagagcgg gggatttcca ggaattcgtc atcaaagagt tcccgggctt
     2761 cacagatttt ccagccatcc tggaatgtca gttcacgaat caagctgctc ttgccggaac
     2821 cgggtccgcc aatgagaatc aacaattttt catcgtcctg ggcaatgtac ttccaccgtt
     2881 ttaaaacatc tgcatctgta accatgaaaa tcaccttcgt tttcttatat tatttatttt
     2941 attataccta actttcatca tataagtaaa gcatttttgc tattttattt tgacatatga
     3001 caaaacaata tgtatcatca tttcaatcca taccgttcca tcgggtcctg gatgttcttg
     3061 atcggcgtcg acgtcaggat gatgtcatcg acatagggct cgttgtgacg cacgacgatg
     3121 accagggaca cgcctttctg atagggactg tcatagtaat aggcgtcgac ggatttttct
     3181 ttgtccggcc cccattcaac gcggtccggc cggccatata aggccaggat cgtcgagaca
     3241 tgataaccca gggtcgcccc gcggccagta taggcattat tcgtcaagag gaagactttc
     3301 tgcacgatat tgcgccggtc cacttcgacg ccgtaatcgc catagagcca gaagttgctg
     3361 ttattgcgct tgaccaggtt ggtccacatg ttgtacttgt tcggctcgaa aggctggttg
     3421 aggccaaaac ccatgagggt aaagtcccgt tcgcccagtt tcatgcggtc cgccggttcg
     3481 tcccccgtaa tgtacggcgg ccgcacgggc atgagggcca cgcgttcgat gcggcggttg
     3541 ccgatggcaa agacgtacat gacatctttc cgcggacttt cataagtgaa gtaatagaca
     3601 ttggcatcgg catcgcgcag gaccgaatcg ggcaggccat attggcgcag cagcacttcg
     3661 gccggatatt tcaggcgtac gtcccggccg attaggatgt tcgtcccagc cgacaggaac
     3721 atcgattccg caccgggcag gacggctttg cgcgtatcgg cactgtggcg caggctgtca
     3781 gccgtcttgt tgcggaaagt aaagcgcatc ttcaagtcct tgtcgttgta ctgcatggtc
     3841 gtatagtggg tgctgcggga aaacttcgcc ggcggcccaa aaatgcgctt gaccttggcc
     3901 gggctgtcgc cgtgatggat gctgccgatg taaaaatcac tggcctgcag gggcggaatg
     3961 gtcagcgtat ccagcgaatc gaggaggacg cagtcatcat ccgtcggtac atgggccgtt
     4021 tcttttttgg tgtctttatt ctctttgacg tctttatcgg atttatccgc tgtgccggtt
     4081 ttgtcatcgg actggggcgc gacggccttg aaggccgtat
//
LOCUS       DAWCYS010000138         4110 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1188_length_4110_cov_5.544513, whole genome shotgun sequence.
ACCESSION   DAWCYS010000138 DAWCYS010000000
VERSION     DAWCYS010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_4110_cov_5.544513"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          759 a         1139 c         1287 g          925 t
ORIGIN      
        1 gagcaggacg tagagccgct tgtccgggct ggtctgcgca aagacgcagc tgtcgctctg
       61 ggtctggctc gggtccggct catcctgctg gatcggcgcc tctttgatgg actggtaatg
      121 caggtacagg gaccggcctg ccaggacgat ggcgatggcg gcgacgacgg ccaggatgcg
      181 gtataatatc tttcttcgtt tctcctgtgt catcctcagg ccttcccatc tgcggcacgg
      241 acgacgtcga tctgggcatt gcggttgacg acggtgccga cgaaaatagg tttgccctgg
      301 tcgaggagat ggcggatcgt cgaatcatag cccaagagga cggcttcatc gaggtcgcgc
      361 ctggctgcct ggcgcaggtc gtcgacaccg tcgaagtcgc ggttcacttc gatgtaatcg
      421 gcgaggaaaa tgattttttc cagggtgctc atctgttccg ccccggtcgt gtggtgggcg
      481 acggccccca agagggccgg gtcggtaatg ccatagcgct ccttggcgat ggtcacggcc
      541 gcatagccgt ggagcaggcc gccgttagcc aggataggtg ccggcaggac gggaaaggat
      601 ttttccgcca gtgcctgcat ctccggcagg ggcagctgtt tggcagcatc gtgcaaaagg
      661 ccggctactt cagccgtatc ggcatcgcag ccgtagcggt ctgccaattc gcgggccgct
      721 gctgctacac ccagtacatg ggtatagcga tgagaagaca gagatttctt taaatcctct
      781 ttgattctat ctttgaattc ttcgttgtac attgatatac tccatttttt ctgatgtatt
      841 cggccacagc cggcggtatc atatagcgca cagatagccc taaacggatg cggcggcgca
      901 gttctgtcga tgagatttcc atggtcggta cgggcagctt gaggatatgc ttccccagct
      961 ctccgaaaga ggcgatgatg gaatcgatct gttctgagcc atcgggccgg gtcgctccga
     1021 cgagctggca cagcttcagg atttcttccg gttccttcca gttgtacagg tcgtggatcg
     1081 tgtctgtacc ggatataaaa tacaagatat acgacggtcc gtagagcttc ttcaggaagc
     1141 gcagcgtatc gaccgtatag gagttgccct tgcggcgcat ctccatatca ctgactgtaa
     1201 aatgcgggtt gtcagccgta gccaggcgcg tcatggccag gcgctgctcg gccgtcgcgc
     1261 cgttgacgtt cttgttgggc gtgatgtatg acggcacgaa gaggaccttg tccaggtgaa
     1321 aggtctggcg cgcttcttca gcgatcatga gatggcctaa atggatggga ttgaaggtcc
     1381 ctcccatgat gccgagccta gtgatttcca tggtaccaat agattcctcc ccaggcgata
     1441 gctactgcta acgcgtaaag caataaaaat ctggcataat gccggtattc aaactgtgtc
     1501 ttcggcgacg cagcgtactg gcgcagggtc cgtccatacc cgcgcagctt ccgcagcctc
     1561 aacggacctg accgtcgccg tcgacgaggt atttatagga cacgagagct tccagcccca
     1621 tgggtccgcg gacatgcagc ttctgcgtgc tgatgccgat ttcggcgcca aagccgaatt
     1681 caaagccatc ggtgaagcgc gtcgacgcgt tgacgtagac agcggccgaa tcgacggcgg
     1741 cctggaaggc agccgcagtg gccttgtttt ccgtgatgat ggcttccgaa tggtgagtgc
     1801 tgaaacggcg gatatggtcc agtgcttcct ggagggagcc gacgaccttg accgacatga
     1861 tgagggcgtt gtattccgtc gcccagtcgt cgtcactggc agccttcatg gcgctggact
     1921 ggcaggcttc gtcgtcaccg cgcagctcga cgccgtcggc tttcatgacc ggcgccagtt
     1981 tcttgagaaa ggcctgggcc acatcgcggt ggaccaggag cgtttccatg gcgttgcacg
     2041 tcgacggccg cgagaccttg gcattttcga cgatcttgac ggccatgtcc aaatcggcat
     2101 ccttgtcgac gtagacgtgg cagacgccgc tccccgtttc gatgcagggc actgtcgccg
     2161 tgtcgacgac catgcggatc aggccggccc cgccgcgggg aatggccagg tcgatatagc
     2221 ggcgcaaggt caagagttct ttgaccaggg cccggtcggt atcctcgatg aggttcacag
     2281 catcggccgg aaggccggct gcggccaggc tttcgcggat gactttggcc agggccgtat
     2341 tggaagcgat ggcttcactg ccgccgcgga gaacgcaggc attaccggct ttgacgcaga
     2401 ggacggcggc gtcgaccgtg acgttgggcc gcgattcata aatgatggcg atgacgccta
     2461 agggcacgct gaccttgcgg atgtgcaagc cgttggggcg gtcccattcg tcgaggacga
     2521 cgccggccgg gtctggcagg gcgctgacct ggcggacgcc ttcggccatg gcggcgatgc
     2581 gctctttggt cagcgtcagg cggtcgacca tggccgacgc catgcccttg gctttagccc
     2641 gctgcaagtc gacggcattg gctgccagga tatcggtttc atgggcgacg agaccgtcgg
     2701 cgatggcggc cagagcctga ttcttttccg ccgtagaggc agcggccagg acgtacgagg
     2761 cttccttggc gtcacggcct ttgcggaata cttcggatat catagaaaat accccctttt
     2821 aatacatggc gacgagatta tcgcgatgaa tgacttcctc ataaatgcca ttgtggccca
     2881 ggaccttggg aatatccgca gactgcaggc ccttgatggc attgatgttg acgctgctgt
     2941 agttgctgat gcccttggca atggtcaggc cgtcgtagac gacgctgacg atgtcccctt
     3001 catcgaagat gccttcgacg gcggtaatgc cgacgggcag gaggctcgaa ccgtggtcga
     3061 tgatggcatt gcggcagccc tggtcgacga cgagccggcc ctgggccttg ctgcccgata
     3121 cgagccagcg cttcttcgag tgcagcggcg tttctttagc tttgaacagc gtccccactt
     3181 cttcgccggc gcagatacgg ctgatgatgc cgtcttcact gcccgaggcg atgaccatgt
     3241 ccacatcggc tgccgtggca atggacgcgg cctgtatctt agtgtacatg ccgcccgtgc
     3301 ccatgctgga cccggcgtcg ccggcgattt catagatgtg cttgtcgatg ttgtcgacga
     3361 cagggatgat tttggcatca tcatgggtcc gcggattatc cgtgtagagg ccgtcgatgt
     3421 cggacaagag gatgagcagg tcggcgtcga cgaggccgct gaccatagcc gacagcgtgt
     3481 cgttgtcgcc gattttgtat tcatcgacgg cgacgacgtc gttttcattg atgatgggaa
     3541 tgacgcccat gtcgagcatc gtcagcaaag cgttgcgaga gttcatgaac ttgctccggt
     3601 cctgggaatc catgcgggtc agcaggacct ggccgacgac ctggccgtat tcgcggaaga
     3661 gccgttcata cgtatgcatg aggacgccct ggccgacggc ggcgagagcc tgcttttccc
     3721 gtatcgaccg gggccggtgg tccatgtgga gcggcgccat accggcattg gtcgcgccgg
     3781 agctgacgag gatcatctgc ttaccctggt tctggagatc cgccagttcc cggaccaggt
     3841 gttccatacg ccgatagttc aattttcccg tagcatggga aatggtgctg gacccgacct
     3901 tgacgacgat gcgtttcttc tggccgaagt ccctgcttgt actgttcaca aagcctcacg
     3961 tcctagtctt taataaattc gtaatgagat ttcttgtctt tcttcttgaa taagacgccg
     4021 tagtggccga tgacctggat gagttcggcg ccgagcatct cggccacatg ttccagggtt
     4081 tccttcgtcg gcagcttggc gttggtgagg
//
LOCUS       DAWCYS010000139         4075 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1201_length_4075_cov_5.007960, whole genome shotgun sequence.
ACCESSION   DAWCYS010000139 DAWCYS010000000
VERSION     DAWCYS010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_4075_cov_5.007960"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1164 a          984 c         1050 g          877 t
ORIGIN      
        1 actgggaaaa tgattccgcg gccaaagagc agaccaggac catcaagcac attgacatga
       61 ttgaggatgc cagccctgtg gatttcccag cctacgatgg caccagcatt gccgcccgtg
      121 gccatgatgg catcatcgaa gagttgaagc gccatgaaca ggacgcggca ctccgtgaaa
      181 agttgattgt tgaaacgtat ctatgagaaa aggagacaac tatgaataag agactttttg
      241 aaatcagaag ccgtaaagaa gaaatcagag ccgcgcttca gggtgacggc aaggtcgacc
      301 tgaaagcact ccaggaagaa ctgagaaagc tggatgctga acagaaagaa attgaagaac
      361 gagaaaagat tgccgaaggt atcaatcttg gaaacaaccc ggaaggtgta catggccgcc
      421 ggaagccgca ggacaatgga gcggctgctg gtattgactc tgatgaatac cgcaaggcat
      481 tcatgaacta cgttgtacgt ggtattacca ttccggcaga attccgcgga gcaactacta
      541 cgactgatgc gggcgcactg attcctccga ctactcttaa ccgagtcatc gaaaaactgc
      601 gtacttatgg caacatcctg ccgctggtaa cccgcactgc gtacaagtct ggcctggcca
      661 ttccgactgc cgacgttaag ccagtagcta cctgggtagc agaaggcgct acgtctgata
      721 agcaggaaaa gacccttggc acggttactt tcagccactt caaactgcgc tgcgcggtcg
      781 ctgtaactct ggagtccgaa tacatgactc tgtctgcatt tgaagatacc attgtttcca
      841 acattgcaga ggctatggcc gttgcgctgg aagaagccat tatcaagggc actggctctg
      901 gccagccgac cggcatcatc aaagacctta ccaagggctc tactctcagc gttaccaagc
      961 tgggccttga tactatcacc ggcgcagaag gcaaggtccc gcaggcctat gaagctggcg
     1021 ctgtatgggt catgaccaaa ggcaccttta tgcagttcat ggggatgacc gactctgccg
     1081 gccagccgat tgcccgcgtc aatgcaggcg tcaatggcgt cccggaacgt gtcctcctgg
     1141 gtcgcaatgt agttctctgt gattatctgc cggacttctc cgcagcactc actaatagcg
     1201 atgttttcgc tttcatttat cgcatgaagg actatgtcct caacaccaac tacaacgttt
     1261 ccatgaaggt ctatgaagac aacgaaaccg acgacctggt aagaaagtcc atcatgattg
     1321 cggacggccg cccggttgac ttcaactccc ttgtcctgct gactggtaat aagacagcgt
     1381 aaggagtgac gaaccatggc cgtaacgctt gcccaggcaa agaattattt aaaactagat
     1441 aatgacatta ccgacgatga tgaactggta acgagcctga tcagtgcggc cggcgactat
     1501 gtaagacgga cgacggggaa ggtcaatact ggcgataatc aaagccagct atatgacctc
     1561 tgtatcaaaa tgctggtggc gcactggtat gaaaaccgtg ctgtttacag ccagaagccg
     1621 ggcgccatca acgcgattcc ccatactgtg accgctttgc tgactcatat tgcccagtgc
     1681 agtgactacc cggagggata gcctatgatt aatgttgaaa tcgggtcact cgataaaaga
     1741 atccatatca tgcagtacca ggaaagtacc gatgaatatg ggcttaccca taagaccctg
     1801 gctgatgcta tcggcaatgc catttgggcc cgtatcgaac cggcacgcgg caagacctac
     1861 tatgagcagt ataaagacaa agttgagttc gtcaccaagg tcacgattcg ttaccgggaa
     1921 gggattaccc cggatatgct ggtgcagtat gcgggcacta cctatcggat catgtcggtt
     1981 gttgacccgt atgaggccca cgtaaaactg gaactcatgt gcaacataaa ggagcgaggt
     2041 gattctggtg aagattgaag aatttgtcag gcggctggat gaaatgagaa tccaatatcc
     2101 gggcgacgcg gaagacgtgc tggaagccgg cgccaagaag atgacgaagg ccatccggaa
     2161 ggcgtcccct gtcggggata cgaaccaccc gcacaaattg aagaagtcgt ggcgctgcaa
     2221 gattaaggga tacagggcgg cagatacccg cgcggaaatc cgttctacgg cacctcattt
     2281 ccatttggtg aaccgtggcg tgcagaaccc aaaggatacg catggaaatc cgaaaccgga
     2341 atggcgcagc gctttgaacc gtcataaggg attcttgcag aaagcggtgc aggacaactg
     2401 ggacggcatc aaggatggca tggccaaaga cttctaccag aaagtacgtg atcaccttgg
     2461 ctaagatcgt aagacagatt gatgcactga gcgctgttat ccaagccgtt tccaaagcaa
     2521 ccggatgcaa agtctattcg gatgaggtcc ttgagaagtt caagaagccg tgctttttcg
     2581 tttcggcatc gtcccgcatg actccgtata cggataatgt agtagagaaa gagctgacca
     2641 ttgccctgac gtatttcccc agggataacg aaaagaatga aataacctac ttagggattg
     2701 ttgacctcat tcagcgcctc ttccagtctg gcgttcaggt tggggaccga tatcttcacg
     2761 tcgaaagcgt agaggatgac cgtaccgggg aagagcaaga catcttgcag gtaaccatag
     2821 tgattccgtt cttggaacag gttgaaaagc ctgctggcgg gaatgttgaa atcatgggtg
     2881 aagttgaact gaatatccat accatctcca acaaacagcg cagcactact gcggatgaga
     2941 aatggaattc gaagattgat tcaggaacta tatgaaggag tgaagatata tggcaaaact
     3001 cggaatgcct tccgtaaaca ttgcgtttat tgaagccggc attgaagcca ttaagcgcag
     3061 ccagcgtggc attgtggctc ttctgctcga agagccgcag gataccatca caaaattgct
     3121 gactgaccat aaaattacta gtggcaccac ttcaaaggat gtggctgcca tcaccaatcc
     3181 gtttaccgtt tataccaccg atgatattcc ggaggaactg actgacgata atcgtgatta
     3241 catcacgaaa tgtctcattg ggtatactaa gacgccatac cgcgtcaaag tgtatttgca
     3301 ggcaaccaat gagacaaaag acagctctga ggataagttc gcagatacgc tcaagatcct
     3361 ggcaacagac cgctgggatt atctggctat tccgtccatc gctacggccc agttggaatc
     3421 tggagccacc tggctcaaga cgaaccgtga aaacaagttc aagaaatcca aggttgtgct
     3481 gcccggatac agcgcagact atgaaggcgt catcaatttc tcgaacacta ccatcaatac
     3541 caaaactaaa tcgtatactg gcgctcagta cacgccacgt attgccggcc tgattgcacg
     3601 aaccccgatg acaatttctg caacgtatgc cccgctggcg gaagtcattg actgcgataa
     3661 atacagcctt gacgaaaacg acgagaaggt taataagggc gagtttttcg tttggtatga
     3721 cggcgaaaag ttcaagatga gccgtgccat gaactctttg gtcactacga ctcaggggaa
     3781 actggaagcc tatcagacca tcaagaccgt ggatgtcatg gacatgatgt atgacgacat
     3841 caagaaaact gcccaggaca gctacatcgg caaatacacg aacgattacg acaacaagca
     3901 gctgctgatt actgcgattg gtggctattt caaagaactg gaagatggcc gcctcttaca
     3961 gaaaggctat tcaacgattg atatcgacgt agaagccgta acaacgtatc agatcgaaca
     4021 tggactgtat acgaaggacg cactagccga catgtctgat tagtaaatta agaaa
//
LOCUS       DAWCYS010000140         4069 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1204_length_4069_cov_6.224963, whole genome shotgun sequence.
ACCESSION   DAWCYS010000140 DAWCYS010000000
VERSION     DAWCYS010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_4069_cov_6.224963"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          988 a         1075 c         1099 g          907 t
ORIGIN      
        1 cggtgaaatc acgttgcgcg ggacggtcct gcccgtaggg ggcatcaagg aaaaagtcat
       61 cgccgctcac cgggcaggca tcaagaagat cctcttgcct gaagaaaaca agcgcgacat
      121 ggacgaagtg ccgcagtcgg tcaaaaaaga cgtcaaattc gtcttcgttc accatatgga
      181 tgaagtgctg gctcaggcac tggtgaagtc ctgatggctt cgtatgatat catcaaggcg
      241 gcctatgcca cgacggcggt ccgccgcgac cagtaccccg aagggaacgt gccggaagtc
      301 gccttcctcg gccgttccaa cgtcggcaag tcgagcctga ttaattccct gtgcaaccac
      361 cgcggcctgg cccgtgtcag cggcgcaccg gggaagacgc agactatcaa ctacttcagc
      421 gcagccctgc ggcggcgcga tgaagaagaa ggggagcagc gcctgccgtt ctacttagtc
      481 gacctgccgg gctatggctt tgccaagact ggcggcagga accgtgacat gtggagtgcc
      541 ttcatcggcg aatacgtcac gcagtcgccg aacctctgcg tcctctgcct gctcgtcgac
      601 ctgcgccatc ccggcctggc catcgaccag caggcctatg actggctggc cagcgccggc
      661 gttcccctgc agattgtagg gaccaaggcg gacaagctca aggccaatga aaagaaccgc
      721 aacctggccc agctcaacaa actcttcccg tcagaccggc cggccctggc ctactcgtcg
      781 ctaaaacgcg acggcttcgg ccccctggcc aagaacctct ttgaacgggt gctggccgtc
      841 agcaggtagc agttagccgc tagccacgaa ccacgaaccc ctaactgcta accccctaaa
      901 aaggggctgt cttcaaagga cagccccttt tttgctcctt ttatagcgaa cgatgtttct
      961 agttggcttc attaagcgaa agtggtataa tggatttgat ttacatcggc cagatgggag
     1021 gttgtataat catggcagta gtttcgattg acagtgatgc tgatttttct gtgaaagtcc
     1081 ttcatcagcc gggcattgtc atcgcagact tctgggctcc ctggtgcgaa ccgtgcaaga
     1141 tggttcattc ggaaatcagc caggctgccg cgaattttgg cgatgctgtg aaagtagtac
     1201 gcatcaatgt cgatacgatg cggaatattg ccgaaaaata tgaaatgatg gcggttccga
     1261 gatgattgtt ctttaagggc ggtaagccac tcaaacgcat tatcggctat gcctcgcagg
     1321 cggaaatcag caagttcctg gcatctgtcg tagcagaaaa caaggctgcc ggttttgaca
     1381 acaaaaatat tgggatgata taattgaatt cagaaacgag agggacggga cgacagttat
     1441 tgtctattgt cgttcctgta tacaatgaac agccgaacat tgagaaattc tatgaagagg
     1501 cgacgaaagc cgcagcggca ctcgatatgg attatgaaat catctttgtc gacgacggtt
     1561 ctaaagatgc aacgcccctc atcctcagcc gcttgaccca gcaggatgag cacgtgcggg
     1621 cttttatcct ggaccgtaac ttcggccatc agctggccat tacctgcggc atggatcatg
     1681 cccttggcga cgccgtcatc accatggatg gtgacttgca gcatccgccg tcgatgattc
     1741 ccgaattggt ccagaaatgg cgcgaaggca acgacgtcgt ccagaccata cgggaagcga
     1801 cggacgatgc gggcttcctc aagcgcataa cgtcgaaatg gtattatcgt atactgaatg
     1861 ccgtatcccc ggtccacatc acaccaggcg ggtccgactt ccgcctcatc gataagcggg
     1921 tcctggaaac attcaagcag ttccgcgaac acgaccgttt catccgcggc ctcatcgggg
     1981 acatcggtta cacacagact tttattcatt tcgtagcacc caagcgttat gccgggaaat
     2041 cgaagttttc cgtacgcaag atgcttcatt ttgccctgga cgggatcatg gcctattcca
     2101 agattcccct gcgggtttcc ttttatatcg gcatgctgag cggtttgttg agcctgctcc
     2161 tgattttgca tgtcatctat tgtaatataa tgggcgaagc cgtgccggga tggacgacga
     2221 cgatgatcgt cgactgcctg ttcagtgggt tccagctcat ttttattggt gtcatcggag
     2281 agtatatagg acgtatattt gaagaggtga aacataggcc tctgtattgg ctgcgggctg
     2341 aactcggcca gcataaagac aggtaggaac ggtgcgttgt atggccttcg agacagagag
     2401 gagtatttat gtggaaaaaa atcagtaaag tttggatatt tgccgcactt ttcgtttttg
     2461 ccgcactgcc ggcgctggca gcttctccgc tgaccattgg cagccggggc gatgaagtcc
     2521 gttcgattca acagattctt cgcagtaaag gctaccgggt caatgtcaac ggcgtttaca
     2581 ccaatgatac ggcgcgggcc gtatcgcagt atcagaaaga taagaaaatc caggtcagcg
     2641 gtaaagtcgg cggctggacc tattatctcc tgacaggcaa tcgttccatg ctgccgccta
     2701 agccggtcat gaagagaggg caggggacgc gcttcaacaa tggcgctatc agcaaatacc
     2761 gtcatttcag cggcggcgat gctttcggca accagctggt cgattcggca taccagtatt
     2821 tgggcatccc ttacgtattc ggcggcaata cgccggctga aggcttcgac tgctcaggct
     2881 ttacgaaata tgtcttttcc cataatggca tcaaactgcc gcgcctggca gatgaacaat
     2941 atgaagtagg ccagcgggtc cgccgcagcg aactcatgcc gggcgacctg gtcttcttca
     3001 cgacgtatga accgggtgta tcccatacgg gcatctatgt cggcaataac aatttcatca
     3061 gtgccaccag cagtggcggc atccgcgtag acagcctcga cagcggctat tggtcgccgc
     3121 gctatgtcgg tgctacacgg gtccggagat aggcaaactg gctaggaaac agtcgtcctc
     3181 tagcctttcc ttatacaacc gggctgtgag ataaaaaatc acaagaaaag gttgccgaac
     3241 agggggaaat gagcgtataa ttttcccatg tagaaagata tttagaggga gtgtttacta
     3301 tatgaagtac atgacgggta atgaaatccg caaagcctat ttacagttct ttaagagcaa
     3361 ggagcacttg atcctgcaca gttttccgct cattccggaa aacgacccca gcctgctcct
     3421 catcggcgcc ggcatggcac cgctcaaacc atatttcacg ggtaagctcg taccgccgtc
     3481 gtaccgcgtc acgacgagtc agaagtgcat ccgcactgac gatatcgaca acgtaggccg
     3541 tacggcccgt caccatacgt tctttgaaat gctgggcaat ttctcctttg gcaactactt
     3601 taagaaagaa gccatttcct gggcctggga attcctgacc aaagtcctgg aactggatac
     3661 ggataaattg tatgtcacca tctatcccga tgacaaagaa gcctatgact actggcataa
     3721 tatgattggc ctggctgacg aacggatttt caaattcgac gataacttct gggaaatcgg
     3781 cgaaggcccg tgcggtccgg acagtgaaat cttctatgac ctcggcccgg aacgaggctg
     3841 cggtaaaccg acgtgtaccg tcggctgcga ctgcgaccgc tacctggaaa tctggaacct
     3901 cgtcttcacc cagtacaacc gcactaaaga cggcaagtac gaaccgctgg ccaagaagaa
     3961 catcgatacg ggctgcggtc tggaacgacg ggcttcggtc ctgcagcaga aaggtacgga
     4021 ctgtggaagg gccgtcatct tgccaagcag ggtaaaagtc agcgccggt
//
LOCUS       DAWCYS010000141         4067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1205_length_4067_cov_5.743021, whole genome shotgun sequence.
ACCESSION   DAWCYS010000141 DAWCYS010000000
VERSION     DAWCYS010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1205_length_4067_cov_5.743021"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a         1122 c         1235 g          976 t
ORIGIN      
        1 catattaacg aataattctt actaatgatt ttgacacgct tataatatca tactggccta
       61 aaaaaagcaa gattatttta tgacttattt tgcctgtctg cccatattga tatcgatgat
      121 gcccgacgaa tacggtgcta cctcatactg gccaaagacg aagtggatat tgcggctctt
      181 gtcgagatag aaattcttcg ggtcttctgt caggccgttg aagtacgtcg acaggacgtg
      241 gccgctcagg gtcagtttat tggtgatttt cttgagggtg aaatactttt catcgcccgg
      301 tttaaccagg tcacgccagt tggtgatgcg cttgcccgtc accatatcga agacgacgcc
      361 ggtcttccag ctggtcggat gggcagccct gtcgaaatag agatagcctt catcgaggaa
      421 ggacaaatat ttgccgtcgt tcaaggtgac ggtgtaggtc ttctcttccg tcaatttgac
      481 gcctttattg ccctgctggt cgaaaaaggt cttggcctgc tgctgttcat cggcaaaata
      541 ctgggctatc agctgggcag ctccggcatt ttccgtcatc gtgatggacg ggtattggat
      601 actgaggacg ctgccatcgc tatgggtata cacggctttg ggcgcatcct ggatgacggc
      661 cggcgaagcg gccatggcgg cggcccccat gagccaggcg ccgcataaaa cggtgaggac
      721 tttctttttc atcatcataa tccctcctct ttcatttctt tctattatat catatctatt
      781 gggtcaattt cctcttttca tccgtcccca ggcgaaagaa ataatagaca ttttccgcag
      841 cctggcggtt catgcccggc acggcagcca gttcgtcgat ggaagcggcc ttcatgtcgg
      901 ccagcgtctt gaaagccgtc cacaaggcct tgcgccgctt ggccccgatg ccttcgacgt
      961 ggtccaggat ggatacgagg ctgcgcttgc cccgcaggtg acggtgatag gtaatggcaa
     1021 agcggtgggc ttcgtcgcgg atggcctgca gcagctggag ttccggcgtg tgatggctga
     1081 ggatgatgga atcgctgcgg ccttcgacga agacttcttc gatgcgcttg gccagactga
     1141 tgaacggcgc ttcgcagcca gcctggcgga tgaccggcag ggccgcatgg agctggcctt
     1201 tgccgccgtc gatgatgatc aggtccggca cgggccaatc cttttcgcca tagcgccgtt
     1261 ccatgatttc cgccatggac ttaaagtcat caggcttgcc ctggacggtc ttcagtttga
     1321 agcggcgata ctctttctta gccggctgac cgtgctcgaa gacgaccatc gaggccacgg
     1381 tctctatccc ctgggtatgg gaaatatcga agcactccat gcgctccggc aggcagggca
     1441 gattgaggat gcgcgccagg ccttcgacgg caccggtcgt cttgtctacg tcgtgctgcc
     1501 attgcaggcg tttctgttcc aacaggccgg ctgcattttc ggaagccatg tccaggagct
     1561 gtttcttcgg cccccgctgg ggaatgacga cggtcacttt cctgcccttc tgctggctca
     1621 ggcggcgttc gcatccgtcc aggtcttcca gggccggcac gacgatttct gacgggatga
     1681 aggtcccgtc gctataatac tggtcgacga cggcttccat cagagacggc acgtcgtcat
     1741 cgctgctgtt gtccagggaa aaggtctccc gtcccatgag cttgcccatg cggaccgtat
     1801 aaatctggac gcacgtctgt ccagcctggc gggccaggcc gatgacgtcc atatcgccgc
     1861 cagccgtatt gacgatgttc tgctgttcct ggatgactgc aatggccttg atctggtcgc
     1921 ggtacatggc ggccttctcg aattccatgg cttccgcagc gtcttccatc ttctgctgca
     1981 gcgaccggga aatgccgctg tcccggcctt cgaggatatt cagggcctgt cggacccggg
     2041 ccccgtagtc ggctttggta atatagtgga cgcacggcgc atcacagtgg tgcaagtgat
     2101 attgcaggca cggccggtct gtcggcatgg tccggcacga gcggatatga taggcccgct
     2161 gcatgagttt caggacacgg tggactgccg tcgagtcggc aaagggtccg aaatagcggg
     2221 cgccgtcgcg gctgacgcgg cgggtcatga agatgcgcgg ataatcttcc tggacagtga
     2281 ccttgatata aggataggtc ttgtcgtcgc ggagcatgat attgtaatgg ggatggtatt
     2341 tcttgatgag cgtattttcc aggatgaggg cttctatttc cgtggcgacg acgatggttt
     2401 ccaaatcggc ggcatgactg accatggccg ctactttcgg cgccctgttg gcatcgtggc
     2461 ggacgtaact ggtcacgcgg ttgcgcaggt tgacggcttt gccgacgtaa atgatcttgc
     2521 ccttttcatc cttccagaga tagacgccgg gctgggtcgg caggttgcgg actttttccc
     2581 ggacggcctc gctgacggcc atggcttata cccccttctt gccggccatg agggccttgg
     2641 tccgttccag gacgggcttg aggaactgac ccgtatagga ttccggtacc tggcagattt
     2701 cttcgggcgt ccccatggcg acgaccgtac cgccgccggc accgccttcg ggaccgaggt
     2761 cgatgaggta gtctgccgtc ttgatgacgt ccaggttgtg ttcgatgacg acgaccgtat
     2821 cgccgccgtc gacgagcatc tgcaagacgt ccatgagctt gtggatatcc gccgtatgca
     2881 ggcccgtcgt cggttcgtcg aggatataca gggtccggcc ggtactgcgc ttggacaatt
     2941 ccgtcgccag cttgacccgc tgggcttcgc cgccggacag ggtcgtcgcc ggctggccca
     3001 ggcggatata gcccaggccg acttgctgca gcgtcttcag cttattgacg atgcgcggta
     3061 tgttttcaaa gaactggcag gcgtcgtcga cggtcatgtt gaggacgtcg gaaatattct
     3121 tgcccttgta tttgacttcc agcgtttccc ggttatagcg ggcgccgtgg cagacttcac
     3181 aggggacgta gacgtccggc aggaagttca tttcgatgcg gatgatgccg tcgcctttgc
     3241 aggcttcgca gcggccgcct ttgatgttga agctgaagcg gccggccttg tagccccgga
     3301 ccttggcttc tgccgtctgg ctgtagagct ggcggatgga gtcgaagacg cccgtatacg
     3361 tcgccggatt ggaccgcggc gtccggccga taggcgactg gtcgatgttg atgatcttgt
     3421 cgatgtattc aacgcctttc agggccttga acctgcccgg ccggtgcggc gtgccgtaga
     3481 cggcttcggc catacccttg tagaggattt cattgaccag cgtcgacttg cccgagccgg
     3541 acacgccggt gacgacggtc agggtcccga tggggatgga gacgtttata tgttttaaat
     3601 tgtgctcagc tgcgccgatg atttccaaaa aggtgccatt cccctggcgc cgttcttgcg
     3661 gaacggggat atacttggtc cccttcagat actggcccgt caaagactga gggcaggcca
     3721 tgatgtcatc ggccgtgcct tgggcgacga tagtgccgcc gtgttcccct gcttccggtc
     3781 cgatatcgac gatataatcg gcagcacgca tcgtgtcttc atcgtgttcg acgacaatga
     3841 gcgtattgcc caggtcgcgc aggcgcttga gggcttccag gagcttgtcg ttgtcccgct
     3901 ggtgcaggcc gatggatggt tcgtccagga tatagaggac gccgaccagg cccgacccga
     3961 tctgggtggc caggcggata cgctgggctt cgccgccgga cagggtgccc gccgtccgcg
     4021 acaatgtcag gtagtccagg ccgacggtct gcaagaagtt caggcgg
//
LOCUS       DAWCYS010000142         4055 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1208_length_4055_cov_5.299000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000142 DAWCYS010000000
VERSION     DAWCYS010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_4055_cov_5.299000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          979 a         1199 c         1141 g          736 t
ORIGIN      
        1 ggtccaagac cggtgcgacc aggacattgg ccaccagcga atagacggga aaagaaaaga
       61 acgccgaaaa caagaggggc acgaccagaa tctgggccgc cacacagaca gtcaggcaat
      121 cccgcaaaaa caaaggcaga cccgcaaacc aggagcttac gggccgctga aaaagaatga
      181 tgcctgccga ggcgccacag gacaggcgga aactcaagtc aaaacaaagg tatgggctgt
      241 aaagcagcat cccggccata gccagggcca gagcatgacc ggccaggtat tcgcgccgcg
      301 ccgtcaggct caaggcactg gcggccccca tgagggacgc ccggacgaca ggcgacgaaa
      361 aatccgacaa cgcgccgtat aagaagagaa aagtcacaga aatacaacac aagctgcgcg
      421 gccgcaatcc caggacgcgg cctatgagct ggatgacggc cagcagcaaa gccacgtgag
      481 acccggaaac agataatata tgaatcagcc cggtcgtact gaatgattcc agcagacctg
      541 gcggcaagtc ctcgtaatgg ccgccaaaga gcagactagc cagtaccggt gcatacgtct
      601 cgccgaggat gacctcataa cgcccggtca aagcctggcg caaatcctgc aaaaaggcct
      661 cccagcgacc tggcggagcc gtcacgacca ccttttcccc atcatcggca aacagccgca
      721 agtaaatcgc tttttcccgg tcgcgatgga gggcgtcgta catgccgtca tttttatagt
      781 atgtcaaagg ccggctgtcg ccagtgacga caaccgtact gcctacgacc tgcggttcca
      841 gcaaagtcac atagatacag ccgtttccag gctgtacgtc cgcttccccg tcataggaat
      901 agcgccggac ctgcataatg taccgtccga cctgtccctt ttcattcgta taggtactgc
      961 gccgttcttg cagcgtccct tccacggtca gcctggcacc agctgcctga tgcggcaggg
     1021 cttcgcagcg ctctgcctcc agctggagac ggccggcgcc cagactaaga gccgccagga
     1081 ccagacagac aactagggca atacggctgc gggaccggaa aaagaatagg cccagcaaag
     1141 acagcaggaa caaagaaatc caaaggctca aagccaaaga aagcacatct gccagggtaa
     1201 tgcccccagc cagggccagg accagggaca ggctgaccca ttccggttta acctttccca
     1261 tcataagcac accaaatctt tcagcttggc gaacttcgcc ggaccgatgc ctttgacctt
     1321 ctgtagttct tcgatactgc caaaagagcc gtgttcctgg cgataagcga tgatattagc
     1381 cgccatagct ggtccgatgc cgggcaggtc cgtaagggct ttctcatcgg ctcggttcaa
     1441 agataccttg ccggcttccg ccggattttc gggaatcccc tggaaatcat agggcacgtg
     1501 gatatgggca ccgtcttctg cgcggtctgc taaattgacg gctcccgtat cggcataggc
     1561 aatcatgccg ccagctgctt ctacggcttc gccagccgtt ttccccgttt ccagcgcata
     1621 gaggccagga ttcttaacgg ctcccgtgac atagacaaag gctttttccg gcaatggctc
     1681 ttctacggcc ggcggtggct cactaaaagc gttttcaaag tcagaggcga attggaacag
     1741 gaccacgcct gccaaaatca gggccaaagc tacgaatttc ttcatgacaa tccctcctat
     1801 acatatactg taagaatcta tatgtatagt atgccctatt ttttaacccc ttttcgaaaa
     1861 tttaatcgtc ctttaattga attcagcaaa atttaatctt tctctcattt accagctgct
     1921 cctggatttc cagaagctct tgcgcagcat gaccaggagg gtgaacattt ccagtcgtcc
     1981 caggagcatg gacaggcaga ggatggattt ggagaagacc gagagttcgg cataggtaca
     2041 ggtcgcgccg gtgatgccga aggccgggcc gatgcagccc atggtggaaa cgctgatgcc
     2101 gatggcgtcg aaggtgttga tgccgtcaaa ggtcagcagc agggcccaga gggcgatgaa
     2161 catgatatag aggaagcaga actgggccgc ccggatgacc gtatcgtcgc cgacgcggtg
     2221 gccgttgatg gtcaggcccc agacctgatg gggatggagc ttggctttgg ctgcctgcca
     2281 gatgtagcgc accatgatga cgacgcgcga gactttgagg cccgctgccg tcgaaccggc
     2341 acagccgccg gaaatcatga gcaccagcag gatgcctttg gaaaagggcg gccagcggtc
     2401 gtagtctgcc gagacgaagc ccgtcgtcgc gatggacgcc gcctggaacg acgaatagcg
     2461 cagggctgtc ggcgccgata cgtcgaagcc gtccatgagg ttgaggccga tgaggactgt
     2521 cgccgcggcg atgatcagca ggtaggcctt gaattccgta ttgcgccgca ggacgtgcca
     2581 gcccttctgc caggcctggt agtacaggcc gtagttgccg cccgtgagga tcatgaagaa
     2641 ggtcatccag gcttcgacgg ccgggctctc gaagtgagcc gcactggcgt tgtacgtgga
     2701 aaagccgccg gcggagaccg tcgataaggc atggttcagg gcgccgagga aatccatgcc
     2761 gcagagcagg aagatgacga aggccgccgc cgtgaaggcc atgtacatgc ggaacaggac
     2821 ctgggtcatg tcgtggagcc tgggcagaac ccgctcccgc gtgggccccg tcgattcggc
     2881 gttgtacatg tagaccgtcg actggcccgc ttccggcagc agggcgataa agatgacgac
     2941 aatgcccagg ccgccgagcc agtgggtcag gctgcgccag aagaggaggc tctgtggcag
     3001 gacctccaag tcggggatga ccgtcgcccc ggtcccggta aagccggaaa tgctctcgaa
     3061 caggccgtcg aaatagttca ggtagccgcc gaggcaataa ggcagcatcc ccaaaaaagt
     3121 tgccaggagc cagcccaggc cggtgatagc aatgccttcg cgcatggtca gtttcttcgt
     3181 ccgcacgtgg ccatagcgca ggaaagccca gccagccagg gcacagacgg ccatggtgcc
     3241 cataaaggcc ggccagctgt cttcggcgaa agccagggcc atgagcagtg gcagacccat
     3301 gacgccggcc tgggcccagg cgatccttcc taaaaattga cagattacgt gtaaatccat
     3361 aagcgtccct tcaaaaaata aaatcctttt tattatacca tggctggcga ggaagccgca
     3421 aaaaaacata tcattccata gaatcttata tatatgatat aataagaact actattgaaa
     3481 taaccgacct atctcgtcag aaaggatgac ctccatgact tatgattata tccaggactt
     3541 attgaatttt atccatcaca gcccgtcgcc gtaccatacg gtccaggcca gccgggatta
     3601 tctcgaccgg gccggcttcc aggccctgtc cctgtccgaa gcgtggcaac tggcgccgaa
     3661 cggtgcctat tacgtccccc tctatgattc gggcctcatc gccttccgca cgggttccga
     3721 tgtgcgccgc cacctgcgcc tgagcgcggc ccatacggat ttcccctgtc tgcgaataaa
     3781 atatcggccg gaaatccgcc agcacggcta cctcaagctg aacgccgaag tctacggcgg
     3841 cctgctgcgc gaatcgtggc tcgaccgtcc cctgtccctg gccgggaccg tggccctcca
     3901 gggcagcgat gccttccagc cggaaatccg cctcgtcgac gcagggagac cgattctgac
     3961 cattccccgc ctggccatcc acatgaaccg ccgggccaat gaaggcgtcg aactcaaccc
     4021 gcagaaagac ctgctgcccc tcatgggcct ggacc
//
LOCUS       DAWCYS010000143         4047 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1210_length_4047_cov_4.107214, whole genome shotgun sequence.
ACCESSION   DAWCYS010000143 DAWCYS010000000
VERSION     DAWCYS010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1210_length_4047_cov_4.107214"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3097..3106
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1011 a         1184 c         1075 g          767 t
ORIGIN      
        1 cctcatgaaa atatccgcgc catcgcgacg gaacagggca tcatccaccg gccggatgcc
       61 atatccgtac cggcgttttt ccaacactaa ttcattacag aaagaggtct tactcgtatg
      121 gaatctatta ttcgcgatat caagttagct aaaagcggtc atgaaaaaat cagctgggtc
      181 gatcgtcaca tgccggccct caatgccatt ggcgacaaac tgaaagccga ccagaccttt
      241 aaaggcaaaa cgatttgcgt cagcgtccat ctcgaagcca agacggccta tttggcagaa
      301 gtccttcatc gcgccggcgc caacgtcatc gtcaccggca gcaacccctt gtccacgcag
      361 gacgacgtcg ctgcagccct cgtcgaagat ggcttgaccg tctatgcctg gtatggcgca
      421 acggacgaag aatacttcga tttcctcaat aaatccctgg accatcatcc ggacatcatc
      481 atcgacgacg gcggcgacca ggtccatctc ctgcacacga cgcgggccga tgccatggaa
      541 aatctcctgg gcggcacgga agaaacgacg acaggcgtca accgtctcca cggcctcgac
      601 aaagccgacg aattgaagtt ccccatggtc gcagccaatg attcgtactg caaatacctc
      661 tttgataacc gctacggcac aggtcagtcc acgtgggacg gcatcatgcg cacgacgaac
      721 ctgaccgtcg tcggcaagaa agtcgtcgtc gccggctatg gctggtgcgg caagggctgc
      781 gctatgcgtg ccaaaggcct gggcgctcat gtcatcgtca ccgaagtcga cccgagcaaa
      841 gccatcgatg tagttttcga aggttttgaa gtcatgccca tggctgaagc cgctaaagtc
      901 ggcgatatct tcgtcaccct gactggcgac aaggacgtca tccgtggcga acacatgaag
      961 aacatgaaag acggcgtcgt cctggccaat gccggtcact tcgacgtaga aatcaacaaa
     1021 cccgaattgg aagccctggc agtccgccac tttgaagccc gccacaacat cgaaggctat
     1081 gaactcccga acggccatgt catcaacctc ctggctgaag gccgcctggt caacctggct
     1141 gccggtgacg gccatccggc tgaaatcatg gacctgtcct ttgccgtcca ggccctggct
     1201 gctaaataca tcctcgaaca ccatgccgaa ctgggcaatc atgtctatgt cctgccccat
     1261 gaaatcgaca cggaaatcgc caagatcaaa ctggcttcca tgggttatgc catcgacacg
     1321 ctcagcgaag aacagcgtaa atatttgaat ctcgactaag gaccaggagg ggaaacgatg
     1381 aaaaaactga ttcagcacgt agccctctac caggaccacg gcgtcttaga aggccagaac
     1441 atcgccatcg acggctcgcg catcacggga tttcccgaag cgccgaaggc atcggactat
     1501 gatgaagtca tcgacggcaa gggcatgctg gccctgccgg gtttcgtcaa cacccataac
     1561 catgtagcca tgacggtttt ccgcagttat gccgacgaca tgcagctcat ggactggctg
     1621 gaaaagaaaa tctggccggc tgaagccaaa ttgacagacg atgtcattta cgcccagtcc
     1681 atgctgggca ttgccgaaat gatccgatgc ggtacgacgg cctatgccga tatgtacgac
     1741 cacatggacc aggaagcccg cgccgtcgaa gaatcgggta tccgcgcctg cttgtgccgc
     1801 ggcagtatcg gtatcgggcc gaatgcccag gccggcctcg atgaaaaccg ccagctcttc
     1861 ctcgactggc acggcacggc cgatggccgc atcacggtca tgatggggcc gcatgcgccg
     1921 tatacgtgcc cgcctgatta tctccataaa ttcgtcgacc aggcacggga actgggagcc
     1981 gaaatccata tccacctcag cgaaactaag ggggaagtgg aaaacatcaa gcagcaatac
     2041 ggcaagtcgc ccatcgccct gatggatgaa atcggcgtat tggactgcgg ctgcctggct
     2101 gcccactgcg tctgggtcga tgatgacgat atggatatca tggcggcaaa gcacgtccgc
     2161 gtcgcccaca atccgggcag caacctcaag ctggccagcg gcatcgcgcc ggtccccaag
     2221 atgctggcca aaggcatcac tgtcggcctc ggtaccgatg gggcatcgag caacaacaac
     2281 ctggacatcg tcgaagaaat gcgcctgact acgctcatcc acaaggccaa taccctggac
     2341 ccgctggtca tcccggccga aacggccatc aacatgctga ccgaaggcgg cgccaagtgc
     2401 ctgggctaca aagacgtcgg caaattggaa acaggctata aagccgacat cactctcgtc
     2461 gaccgcgaag gcctgcactg gtaccccaag aacgacaccc tgagcctcat ggcctacagt
     2521 gccaacagca tggatgtcga tacggtcctg gtcaacggcg aagtcctctt gcgccataaa
     2581 gaattcacca aactggacat cgaaaagatc aaagccgaag ccgaacggac ccggaagaaa
     2641 ctctttgccc agatttagtt ttagtggttc gtgactcctg tagggggctt gcacgtggca
     2701 agcccgccgc aggttatgtt ggaatttcct cattagcttt ttgggggaga atcgtacata
     2761 tcctgacctt attaagcctt gcaagtccac cctttccttg tataatggaa gtatagattt
     2821 ccagtacagg ggaagggtga tttttatgca tatgaagaaa aaaatagccg ctgcctgtgt
     2881 ggcagccatg gccgcgttct cactgcctat gggcatcgtg ccggtccagc cggtccaggc
     2941 tgacctcatt accgatgtca tcggcggctt ccaggtgaaa tcggccttga gcaagcagat
     3001 caagcactat gacacgccca tcggaggcga gggggcgggt gccccgtccg tgaccgagga
     3061 gggcggggtg ctccgtagtt ggtagtgcgt gggaggnnnn nnnnnncgtc ctcaataatt
     3121 cttattacgc tgaacggctg gcttctatca tgaaccgcct cagcgccagc atcgcccaga
     3181 cggaccccag catcaagacc ttgccctacc gctggtatgt cagcccggac acgacgttca
     3241 atgcggcctg caccatgggc cacgtcatgg tcgtcaataa gggcatgttc gacctggtca
     3301 gcaacgatga tgaaatcgcc gtcgtcttag gccatgaaat gggccacggc cagaaacacc
     3361 acgtcgccaa ttcgacgcag aagaaggtca atgtcgaaat cggcaagatg gtcctggccg
     3421 atacgattgg cggctcgggc ctgaacaacc tcatcctcaa cacggtcagc aatcagatcg
     3481 aaacggtcca tatcgacagg gctgccgaat gggaagcgga caacctgtcc ttcgggtata
     3541 tcacccgtgc cggctataat cccggtgcga cggccgccat ctggcagcgg gtcatggaaa
     3601 aacagggcga taatgccagc aactttgtcg gtgaaatctt ctctccctct gaccatccat
     3661 cgaatcagga tcggcgcgac aattatgcca acaagctcta tgaaatgagc ggcaaacacg
     3721 ccagcgtcaa agacggcacg gtctacgtca acggcaacaa attcatcaag cccgcagcca
     3781 cgtccagcat gagcagtgcc gaacggtcct atttcgtcct gggcaacctg gccgcagcct
     3841 accagaacgg ccacagcaag cagcaggcca cggcgtccgg cggtacgctc tacttggggg
     3901 cgcagaacat catgacgccg gtcaacggcg acccgtcggc agaagaattg gcgacgcagc
     3961 tcaataaaat taaataatga ctgaaacgaa tagtaaaggg cggaatcgcc aggtactatg
     4021 tatattttat atctatcacc tgtagta
//
LOCUS       DAWCYS010000144         4042 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1212_length_4042_cov_5.794332, whole genome shotgun sequence.
ACCESSION   DAWCYS010000144 DAWCYS010000000
VERSION     DAWCYS010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1212_length_4042_cov_5.794332"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          821 a         1184 c         1193 g          844 t
ORIGIN      
        1 ctgctggcgc tcactggaac cggccaggag gagcgtgcgg aactcgccct gcggcagcag
       61 gacgacctgg cggaactggt cggccttgaa gcccagcagc ctttcgacgg cagccgtcac
      121 gttcttggcg gctacgagct gttcctggcc gccgtctttg tccgattcat ataaggccgc
      181 gtgggccgcg gctttcttca ggcccgtgcc gcgcttcttg gcgatttcct gttccgggct
      241 gcggtcgatg cggtagtatt tccggccgat ggcaaagcgg aaggacacgg ccgtcgcttc
      301 ctggggcgac gcgtactcac tgcgcatgtg ggcgccagtg cggctgtcgc cgctggtgct
      361 gccgtagagg gcgtagcaca tggcatcgag gatggtcgtc ttgccggaac ccgtcggtcc
      421 atagatgagg aagagtttgt ggtcgcccag ttcgcggaag tcgacgattt cctgaccggc
      481 ataggggcca aaggccttca tttctaagta aataggaatc ataacaggct gcctcccttt
      541 tctaattcat cccacaaggt gcggacgcag tcctgctcgg cttcggtcag gggctggtcc
      601 ggccgcatgg cctgggcgaa gctggtgaac agttcctgtt ccgtcacctg ctgtacgtcg
      661 aaatcgcgct gtcctgcggc ccgggtattg cggttgggca tttccatcgc catggcattg
      721 gggtatttgc ggcgcaggcg ggccatgccg tcgaggatgg gctggctgtc gtcgaggcgg
      781 gccaggatga agtcgtccgg cctgtcgtca tcggccgcca tgatggcctc gaaggttccg
      841 ctgacgatgc gcacgtcgtg gcgcggcacg aaatcccgga aggccaggtc cactttcccg
      901 tcgggcccga tgtcggcgat gacggcgcct ttatgctggc gcgcttcgcc gaacgagtat
      961 ttgagcaggc tgccggcata gcggatggta tcgctcgtga ctttctgcgg cccgtggaga
     1021 tggcccaggg ccgtatagct gaacggcgca aagaggccgc tcgtcacctg gtccgtcccg
     1081 ccgatggaca agggccgttc cgaatcactg gctacgccgc cggcgacgaa ggcatgggcg
     1141 acgcagaccg tgcgcacacc gtccgggacc tggctgccct gccagccgag gagggcctcc
     1201 atggcctgct ggtggctgcg gacctcatcc tggccgaggc actggcggac gacggccggc
     1261 tcggcatagg ggacggtcag gaaggcgacc ggtccggcgt catcttccag gacgaccggg
     1321 cccgtaaggc gctccagttc accggccata tagaggccct gcggcgccag gaggcggctg
     1381 gcaaaggaca ggcgggccgg gctgtcgtgg ttgccgctga tgaccaggaa ggggacgtgc
     1441 aaatccgccg tgatccgcgt ggccacgtcg tcgaagagtt ccaccgcttc ggccggcggc
     1501 aggctgcggt catagacgtc gccagccagg acgacggcgt cgatgccctc gtcccgtatc
     1561 atgggcagga gctgctgctc caggacgtag gcctggtctt cggtcagata gttcccatag
     1621 aataattttc ctaaatgcca gtcggctgta tgtaagatac gcatagaata cctccttacc
     1681 aagctactgc tgcagcgacc gcagcagggc gatttccctg gcatagcctg ccatatcgtt
     1741 gggtgtttcc agataaaagg gcaggtcccg cagggccgga tgattgacga tggcccggaa
     1801 ggcttcgagg ccgatgtggc cctggccgat tttttcatgc ctgtccttgt ggctggccag
     1861 ctcgttcttg ctatcgttca cgtggacggc tttcaaccgg tccaggccga tgaccttatc
     1921 aaaagtcgtc aatacgtcgt ccagatggtg gacgatatcg tagccgccgt caaagacgtg
     1981 gcacgtatcg aggcagacgc cgattttatc gttcatggcc gctttgtcga tgatggccgc
     2041 cagttcttca aaggtccggc ccacttccgt ccccttgccg gccatggttt ccaggaggac
     2101 cgtcgtcttc tggccgggga acatacaggt gttgagcatg tcggcaatga gggcgatacc
     2161 cgtttcagcg ccctgtccga catggctgcc gggatggaaa ttatagcggc agttgtcgac
     2221 gtattccaga cggcgcaggt cgtcggccat ggcctggcgg gcaaactccc gggtcttctc
     2281 cgtctttgaa cagccgttca aggtatacga cgcatgggcc aggatgggcg cgaagtgatg
     2341 ggctgccatg agctggttca gggcctgtat atcgtcgacg tccagtttgc ggacgccgct
     2401 gccgcgggga ttgcgggaga agaactggaa cgtatctgcc cctatagata aagcttcgtc
     2461 ccccatgtgc aaaaatccat gtgaaaccga taaatgacat ccaatatgca acatattttt
     2521 tcctcctgcg cgacggataa caaaaaaagg ctgccgcacg aagcccgtcc ggcctcatgc
     2581 agcagcccct atccttagac tatatgaaaa tcaaatactt acgatttttc ccgacattcc
     2641 gggcaaatcc cgacaaacga aatactgacg ccgttgacga cgaagccgct ctgttcagcc
     2701 gcctgctgac gcaatccctc ctggtccatg tcgatatcga tgacgcggtt gcacttttcg
     2761 caacgaatat gggcatgggg agttatatta taatcatagc gatgggcatc ttctgcgcac
     2821 tgcaagacct ggacaacgcc aattttggca aagatttcca tggtcttata gaccgtggcc
     2881 agactcatgg acggataatt gggctgcaat atcttataaa tcgcctcggc attgggatgc
     2941 gtcggattgt ggttaatggc ttcgtacaca gccaggcgct gcggtgtgac tttatagccg
     3001 ttttttcgca aaacttcagc aatttccatt tttatccccc tttcttggaa aactgtggta
     3061 taataagaca ttctagtttt attctagctt tgaatcatat agaatactgg agagtatcct
     3121 gtattatatt gtaacggtta ttaagaaaaa tttcaatgtt ttacgcgaaa ttcttcttat
     3181 acaataagta atattaacta ttttgttagg aaggaatgta atcatgacag aggaaacgat
     3241 gatcgctgaa atcgccagga ctctgggtat ccgcccggcg caagtccaat ctgccctttc
     3301 cctctttgcc cagggcaata ccatcccttt catcgcccgc taccggaaag aagcgacagg
     3361 gaccctcgac gaagtacagc tgcgctgcat ccaggagcaa tatgaatacg aacaggccct
     3421 ggccagccgc aaagaaacgg tccgccagtc catcatggac cagggctgct ggaacgacga
     3481 cctggcacgg agcctggaag cggcccggca gctccaggac gtcgaagacc tctacctgcc
     3541 ctataaaccc aagaaacgga cgaaggcctc catggcccgc gacgccggcc tggaaccgct
     3601 ggccgactgc tttgccagcc aggaccccaa gggcccggct ccggaagacg ccgccctggc
     3661 ctttctcagc gatgacgtac cgaccgtcga agacgccatc cagggcgcag ccaacatcct
     3721 ggccgaacgc ttttcggaac gggccgattt ccgccgccac ctgcgccgcg aactgtggca
     3781 gcaggcccgc ctgacctgca gcctgcaagt cgaagaagac caagccggtc ccatgctgac
     3841 ctatgccgac ttcagcgaac gcgtcgcccg tatcccgagc taccgcatcc tggccatcaa
     3901 ccgcggcgaa aacgaaaaga aactgaaagt caccttgaag gagccggcag accgccatat
     3961 cgccatgctc tgccagctgg tcatcaccag gccgtcgccg tatgatcaga tcctgcgcga
     4021 cgccgccgcc gacagttaca ag
//
LOCUS       DAWCYS010000145         4021 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1219_length_4021_cov_5.912002, whole genome shotgun sequence.
ACCESSION   DAWCYS010000145 DAWCYS010000000
VERSION     DAWCYS010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_4021_cov_5.912002"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          877 a         1069 c         1144 g          931 t
ORIGIN      
        1 gccccttggg cgttccgcag gccgcagttc gcagtccgcg gtccgcgtgt agggcgccac
       61 ggattatgac gaataatgca ggccgccgtt cgcatgtagg ggcgccacgt ggggcgcccg
      121 ccacggatta taatgaataa tgcagtccgc cggccgcaaa aggaccctcg catgacggta
      181 ttcgtcatca tgcgagggtc cttttcttgg tcaaaagcaa cctctcagac cgccttcggc
      241 ggccagctcc cctataaggg gagccgctag ccacgggcca ctaaccacta aaaaagggcc
      301 tgtcgcacga aggtttctgc catcgtgcga gggtcctttt tcttggtcaa aaacaacctc
      361 tcaggccgct ttcggcggcc agctccccta taaggggagc cgctagccac gagccactaa
      421 aaaagcgttg tccgtcagga caacgctttt ttctacctta tttcccgctt tcagcgatgc
      481 agtcattgag gtcagcaatg agtgcgtcga cgtcgatgcc gtgggcacca gcgccctggc
      541 cgatggtttc aaagtgagca gccatgcagc caatgcagcc gaggccgtac tgctggaaga
      601 tttcaatgat ctgcggatat ttctgaacgg cttcaatgat acccgtgtct tttgtaatct
      661 gtgccattat ttttattcct ccttgtcaat tcgtatccgt accgttttga aggaacttcc
      721 ggattgcatc tattatatca tacttttttt catttgtgcg aggaaatact tttttcctcc
      781 aagagttggc cgtcgggcag gaagctgcgg aaggtcaggc tgtcgtccgt cgcttccagg
      841 gtcatgtaat tgtccgtttc cggctggggt gcgacgtatt cgtcgaggct gtgctgtttc
      901 cacaatcccg gatactggac gttgccggcg acgccggtga tgaggtacag cgggcccgat
      961 tcatcgcgct ggaagttcct gatatggtcc cggtcgcggt aggtgtggag atgggccgag
     1021 aggacggcat cgacgccgta ctggtcgaag aggggcatcc acagccggcc ttcgggcgag
     1081 aagccttctt ctcggggctg gggccggttg gctattccgt tctcgatcgg gtccttgttc
     1141 atgagtaaga ttatccattt cttcgtcgtc ttggccatgt cttccttgaa ccaggccact
     1201 tcgtcttcgt cgaggctggg ctcgaagtca gccagttcct gggactgcgt gttgaggacg
     1261 acgaagtgga cgtcaccgta gtcgaaggag tagaactggt tctggtattt ttccctgtcg
     1321 atacgaggca gggcgaagag gtgcaggtag gcttcgggca tgcgaacttt ccagtccttg
     1381 tcatacgttt cgtggttgcc caggaggggg acgacgggga tgcgggcgat catgtcgccg
     1441 acgacgtcga accaggcgtt ccactggtaa tggtcctggc cgttgtcgac gaggtcgccc
     1501 atgttgatga agaactgggc gtcctggttg cgctgccagg caggttgggc cgtggcggcc
     1561 cagacgctgt agtcgctgga ctgggaatcc gggaagatga gggccttgaa gtcatggccc
     1621 tgggccgtat ggaaggactg ccagtcactg cgtttgtcgc cggcaccgac gcggtattca
     1681 taggccgtac ccggttttag atcgctgacg gccgccgtgt ggatgtacgt cgtcacgcca
     1741 tcgtcgctga aggcgtcact ggaagcgggg agctgcatga gggaatcgtc gtcgccggct
     1801 tggcggtatt cgacgacagg attctcttcg gcgtagtccg actgccacat gaaggtccgc
     1861 gacgtcgtgc tgtcggctgt gatgacctgg cggatgttcg aggcatcgac ggcatccgtc
     1921 gacaggtagt gggccgccgc ggctttggtg gccttgacgc cctgtctggc gacggtcttc
     1981 gtacccggca gccaggtata ggcactgacg gcggcgatga cggcagcggc cagggaaaag
     2041 gcgatgattt ttttatattt ggctttcata gaattcctcc tgtttttttt gatataagtc
     2101 attataagac atggagtcaa ctccaagtca agcaaaaaag aaaaacttga caaggccttc
     2161 gcttcgatat aagatagatg catcaaaagt gatagagaac gtccatgaac gggagcaagt
     2221 accgtcatga tacccttgac agcgagccgg tggcagtgga agtccggcga agaggccatg
     2281 agcgggaatg ggcccgggag atgcaggctg aacgcgagta ggtcctgccg gaactttcac
     2341 cgttaccagg aaagaatgca tctacgtgtg catatgagcc gggcagtttt gccaaccagg
     2401 gtggtaccgc ggaaatgact ttcctttcgt ccctaatgtg agggatggaa ggattttttt
     2461 agtgaacagg gaggaatcca tcatgtctat tattttttct ggcattcagc cgagcggcaa
     2521 tttgacttta ggcaactatt taggcgcact gcgcaatttc tcgaagatcc aggacggcaa
     2581 cgaatgttat tactgcgtcg tcaaccagca tgccatcacg gtccctcagg atccgcagct
     2641 tttgcatgaa cgtacgcgga aactggctgc catctacctg gcttccggtc tggatccgga
     2701 aaaatcgacg ctcttcgtcc agtcggaagt cccggaacac gcactcctgg gctggatgat
     2761 gattaccttg tcctatgtcg gcgaattgga acgcatgacc cagtataagg acaaggcttc
     2821 caaacggggc gattccatcc cggccggcct gttgacctat ccgcccctca tggctgcgga
     2881 catcctcttg taccagacca acttcgtccc cgtcggcgac gaccagaagc agcacatgga
     2941 aatcacccgc aacctggccc agcgcttcaa ccgcttctac ggcgaagtct tcaccattcc
     3001 cgatatctac ctcggccagg acggcacgcg cgtcatgagc ctccaggaac cgaccaaaaa
     3061 gatgagtaaa tccgacgaca acacgacggc gacgatttac ctcctggacg aaccgaaagc
     3121 catcgaaaag aaaatcaagc gggcccagac ggacagtgaa aattccgtcc actacgataa
     3181 ggagaacaag ccgggcattt ccaacctcat tgaaatcttc tcggccctga ccgaccagac
     3241 ccacgaacag gtcgaagcgg cctacgctgg caagggctac ggcgccttca agaaagacgt
     3301 ggccgatgcc atcatttcga cgctggaacc gatccagcag cgttaccgcc agctcgtcgg
     3361 cgaccccgaa ctggacgcca tcctcgacaa gggcgctgcc aaggcacacg caaaggccag
     3421 cgtcacttac gaaaaagccg ccaaggccat gggtttatac cggaagtaaa aattaaaaat
     3481 atggcaacac aacttaatac tttattttaa atattatatt gacataacaa aaataataaa
     3541 gtattatact ttacacatcg aaaaaataaa taaagcagag gcaacggtgc catcagacac
     3601 tgtgcttctg ctttttttat tttgtaaagt catctgtgtt ataaaggggg agtgccatat
     3661 ggcagcgaaa aagtctagtt ttgacaaagt actcaatgcc tgggacgtcc tggtcatcgc
     3721 cttcggggca atgattggct gggcctgggt cgtatcgaca ggggattgga tcaccggcgg
     3781 cggtgtcctc ggcgccatgc tggggttcgc catcggcggc gtcatgattt tctttgtcgg
     3841 ccttacttat gccgaattga catcggctat gccgcagtgc ggcggcgaac acgttttcag
     3901 ttataaggcc atggggcctg tcggttcctt catctgcacg tggatgatcg tcctcggcta
     3961 tgtcagcgtc gtctgttttg aagcctgcgc cttgccgacg atcctcctct acttgttccc
     4021 g
//
LOCUS       DAWCYS010000146         4004 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1224_length_4004_cov_4.601671, whole genome shotgun sequence.
ACCESSION   DAWCYS010000146 DAWCYS010000000
VERSION     DAWCYS010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_4004_cov_4.601671"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          824 a         1065 c         1159 g          956 t
ORIGIN      
        1 cttacaggct gtcccaacga ctgcgacaaa atccgcatgc atgacttcgg cgtcatgggc
       61 atgacggaac ctcagttcga tgccagccgc tgcgtcagct gcggcatgtg cgtccgcaag
      121 tgtaaacagc tttctgtcga agccttgcag atgaagaatt tccgtcctgt ccgcaatgtc
      181 gaaaaatgca tcggctgcgg cgtctgtgtc tgggcctgcc cgaaccgtgc ctggacgcgc
      241 agcaaggaac agtatttccg tatcaccctt ttgggccgta caggcaaaaa gaacccgcgc
      301 atgggcgtcg atttcatcaa atgggccgat gaagacagcg tcgtgaagat tatctccaac
      361 gtctatgctt ttgccaagaa atatatcgac ccgaccgctc cgggtggcaa ggaacatatc
      421 ggctacatgg tagaccgtac gggtccggaa gaattcatta agttcatcct ccagggcgtc
      481 catctgccga agaaggcgga aatctaccgt cctcttaact ggtccggtat ccgctatgtc
      541 ggcttgaaat cggaaccgtt ctacgtcggc aaagacgtaa atccggccga tctggatgta
      601 ttccagaaac cgttgccgct gaaataagga ggagcctatg tacaacgacg aatacgctgc
      661 gaccgcggcc ggggcagctg cgaaagtagg cctcttgaag aagaatcccc tgggatactt
      721 cctgttgtcg gccttagccg gtgcctatat cggctttggc atcctcctgt cctttacggt
      781 cagcgggacc ttgggagcca ttcccatgac taagatcgcc atgggcctgt ccttcggcat
      841 cgccctgagc ctggtcatca tcgccggggc ggaactgttc acgggtaacg ccttcgtcct
      901 ctacaatggc tggcggtcga agacggtcac cttgacggac gtcatccgcc tgtgggtcgt
      961 ctgttacctg ggcaactggt tcggcgccat cgtcctgtcc ctgctcttct atggcgctgc
     1021 cggcacggct ggcggcgtcg gccaggcttt ggccaatgcc gctgccatga agatgagctt
     1081 gccgccgctg gtcctcttcc tcagaggtat cctctgtaac gtcctggtct gcctggccgt
     1141 ctggtgcggc ttccgctgca aatcggacag cggcaaactg atcatggtct tctggtgcct
     1201 ctacgccttc tttacctgtg gctttgaaca cagcgtagcc aacatgaccg tcctgaccct
     1261 gggtctgctg aatcccgccg gtacggccgt cagcgtcggc ggttacctct acaacctggc
     1321 cttcgtcacc ttaggcaaca tcgtcggtgc cgtcctcttc ctgtccattc cctacggcat
     1381 cgcagccaaa aaagattagt ggctcgtgct cgtggctctc cttataggag agctgtcagc
     1441 gaagctgact gagaggttgc tctttcttta tagtagagta gtccgtgatt ggtaggggcg
     1501 accacgtggg gcgcccgtca gggatgatag gacatatccc ggagcggact cgccacgtgc
     1561 gagcccctac agaaaaggga cctcacacga tggtttttgc catcatgcga ggtccctttg
     1621 ccttatagtt tgtaacaaag agtttgtagt ttgcagagaa aacctttaaa tttaaagcca
     1681 tctgctaacc cctagccact aactgctaac cactaaaaaa ggcgctgtct tcatgcgaca
     1741 gcgccttttt tcaatacagc gtcacccgct gctggtttat ttcgatgagg ccttcgtctt
     1801 tcatgtgagc cagttccctg gcaacagccg tgcggtcggc acagagatag tcagccagct
     1861 gggtccgggt cagggggatg gtgaaggtct ggctgccggc ctgttcagac tggaatgaga
     1921 agtaggtcaa tagtttcttg cgcagcttct tcttggaaac gacttctagt ttggtcagca
     1981 gctgggcatt cttgtcagcc atgagatgca gcatgttctc gatgagctgg tgatggaagg
     2041 gacaggcatt ggtacagatg tgcaggacct tctggaaggg gaggaccagg aactgcgtgt
     2101 tcttgacggc ctggaaggtt acgatggatt gcaggattgt gccgcagacg aaggtttcgc
     2161 taaagaggcc gccgctgcgg atgacggcca ggacggcttt gtcgccccag cggtcttcat
     2221 ggatcatatg gacttcgccg ctgaggacga tgccgatgta attcacttca tcctgggcca
     2281 ggacgaggaa ttcccctttg cggactgtcc ggacccgtgc gcccaggcag gtgagcatgg
     2341 ccttcctttt ttcctggctg atgccggaaa ataagggaag gttatcgggc aggaccaccg
     2401 agaaatcaga catgagaaga cctcccttaa taccgacgat agaggccgat gactttgcct
     2461 aagatttggc aatcccgggt ataaatcggt tcgtattcgt cattttccgg ctgcaggcgg
     2521 atcgtgtcgg cttcgtggaa gtagcgcttg accgtcgctt cttcgccgat gagggcgacg
     2581 acgatttcgc cgttttctgc cgtttcctgg cggcggacga tgagctggtc gccttcgagg
     2641 atgccggcgt tcttcatgct gtccccttgg acgacgagca tgaagcagtc gtcattgccg
     2701 atgaagtccg ccgggaaggg gtagacgtct tcgacacatt cttcagcaaa gatgggggca
     2761 ccggcccgga cctggccgac caggggcagg gggacgattt tcttggaccg ccacgaacct
     2821 tcgtcgagga gttcgatggt ccggttcttg gctgggtcgc ggcggatata gcccagatct
     2881 tccaggcggt ggagatagcc gtggaccgtc gacgtcgagc tcaggccgac ttccttgcag
     2941 atttcccgta ccgacggggg atagccgaat ttatggacac acgtgcggat gtattctaag
     3001 atttgttttt gacgggtcgt cagcattttt tcattattaa tcatatatat cactccagtc
     3061 ttggaaagtt atagcaattc cagtataagc taccttcatt ataccgaaat tcataaaaaa
     3121 aatcaaacgt ttgttcaaaa tatcttgaca ggaacaaccg ttcgacgata taataaaacc
     3181 atcgacgaac ggttgttcta agaaaattcg ttccaggaac gttcgtttta aagtcaggag
     3241 tgattgtcat gaaatatgtc atcttggcca tattggccct ctttatgtgt acgcagatcg
     3301 gctggagtta ccagccttcg caggaattca tttccgtcgc cgtcgaaccg gggcagaccg
     3361 tctggcagct ggcttcggtt acagccggtg atgatacgga tgtgcgccag gtcatggatt
     3421 cgattttaaa agaaaacggc ctgaccggca ccagtgacat ccgtccgggc caggtcctgc
     3481 gcctgccggt agccccaggc cgggccgaac aggtgcggac ggtgctggcc cggcagctag
     3541 tggattagtg agggtgagca tcttcggtaa tctttaaaat cgggctgtcg atgaggcggt
     3601 cggcttcgtc gatgtagcgg cgcacgatgg cctgggactt atgccgggtc tggcgcatga
     3661 tctgccgttc ttcgacgtga tgctgggccg ccgacgtggc aaagccgtgg cgcaggctgt
     3721 gggcgccgta gtcgtcggga ttcatgccca tgagcccggc atagtgcttg acgatgaggg
     3781 cgatagattt atcggaaatc cgggtcttac ggacatgatg ccccttcgtc aatccccgga
     3841 agaccggacc cgtcgtgatg ccgctgacat cgagccattt ctgcaaggcc gtgacggcac
     3901 agaggtcctt gtctttgacc atggggatgg caatcatctg gccctggtcg aactggtccg
     3961 tcttcgattt gcgcagggta atgagcaggc tcaggcgggt gaac
//
LOCUS       DAWCYS010000147         3980 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1230_length_3980_cov_4.657580, whole genome shotgun sequence.
ACCESSION   DAWCYS010000147 DAWCYS010000000
VERSION     DAWCYS010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_3980_cov_4.657580"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1051 a         1192 c          983 g          754 t
ORIGIN      
        1 cgagcagcct ttcgccgtcc agggcatttc cgtagaaaac ttcatccgca acgccaagag
       61 caccgtatcg gttcagcagc agcgcatcat gcctttccgt aagaagctcc acgcccagat
      121 ggaacagctg aagatggata agtcttattc tgaccgctat gtcaatgacg gcttctccgg
      181 cggcgaacgg aaaaagacgg aaatcctcca gatgtgcatg ctcgaaccga aactgaccat
      241 gctcgacgaa atcgactcgg gcctcgacgt cgatgccgtc cgcatcgtat cggacacggt
      301 atccaagtac cacaatgaaa acaattccat cctggtcatt acccaccaca gtgaaatctt
      361 gcagcacttg aaaccggatt tcgtccacat cctcatcgac ggcaagatcg tcaagaccgg
      421 cgatgcatcg ctcatccagg ctattgaagc caacggctac gacgcataca aaggatagta
      481 ggaggtacag ctatggctga taataaagaa aagctggtcg atcaggaaaa agaaaaactg
      541 gtcgaacaga aagaagaaaa aatcgctgaa gaacgccaga agatcgatac cttcgccgac
      601 ttcggcaaca tgtataactt taagaaagat tttacctatt cctataagac cgatgccggc
      661 ctgacggaag acatcgtccg tgaaatttcg gaaaagaaac acgaaccgaa gtggatgctc
      721 gatttccgcc tcaaatcgct ggatatcttc aaccacatgg aatatcccga ctggggcccg
      781 gacatcagcg aactcgacat ggacaacatc gtcacttatg tccgcccgaa cgctcagatg
      841 aaagctgact ggaacgacgt ccccgatgac atcaaagaca ccttcgaccg cctgggtatc
      901 ccggaagccg aaaagacgtc cctcgccggc gtcggtgccc agtacgactc ggaagtcgtc
      961 taccacagca tccaggacga actggtccag cagggcgtcg tctataccga cttcgatacg
     1021 gccctggaaa aatatgaaga catcgtcaag gctcatttca tgaagctcgt cccgccgacg
     1081 gaccacaagt tcgccgccct gcacggtgct gtctggtccg gtggctcctt cgtctacgtc
     1141 ccggccggtg tcgacgtctc catcccgctg cagagttatt tccgcctcaa tgcgccgggc
     1201 gcaggccagt ttgaacacac gatgatcatc gtcgaaaagg gagcgaaagt ccacttcatc
     1261 gaaggctgtt cggctccgaa atacaatatc gccaacctcc acgcaggctg cgtcgaattg
     1321 ttcatcggcg acgatgcatc cctgacctat tcgaccatcg aaaactggtc taagaacatg
     1381 tacaacctca atacaaagcg ggccatcgtc ggcaagaacg gcaccatcaa ctgggtcacg
     1441 ggctccttcg gttcccatac gtcgtgcctc taccccatga gtattctcca gggcgaaggc
     1501 gcacactgcg aattcacagg cgtcaccttt gccggcaagg gccagtacct cgatacgggt
     1561 tcgaaagtcg tccacaacgc accgaacacg acgagcaaca tcaattctaa atccatcagc
     1621 aagagcggcg gcgtctgcat ctatcgcggc agtgtcgtca tcaacccgcc ggctgacggc
     1681 gccaaggcca acgtcagctg cgaatcgctc atgctcgacg aacagtcgca gtccgatacg
     1741 atcccggcta tcgtcgtcaa gaacgacaac atcgacctcg gccacgaagc ttctatcggc
     1801 cgtatttccg acgaagctat cttctacctc atgagccgcg gcctcagcga agatgaagcg
     1861 cgggctatgc tcgtccgggg cttcgtcgaa ccggtatcca aggcactgcc tttggaatat
     1921 gccgtcgaaa tgaataacct gatcaatctc gaactcaaag gttcgttgaa ataggggagg
     1981 aggaacatca tggcagtaaa gacagaaaaa atccagtaca accaactccc gcgcccgacc
     2041 ttccgctgga tgaaggtcaa tcacctggaa ttggacccct tgaaagccca ggctgctccg
     2101 gcttatacgc cggaagaacg ccacgacggc gacgcagcgg tgatcttcta tgaaggcaat
     2161 cagatgccgg gcctcggcga tttccagggt tccaatgcgg aagaactcca aaaagcactg
     2221 aaccaggcca attctggctg cgccgtcagc gtcggcgaaa accagaaagg gtcggtccat
     2281 ttgaaataca gcctcagcga agcgatgcct cagctcacgg ctcagctcaa aatcgaagcc
     2341 gctgcccaca gcgatttgac ggtctacctg gaatttgacg gcagtgccga tgacggctca
     2401 gtcaacttcc tcacttacgt caagaccggc gaaggggctc acgtcaaagt cagcaaggtc
     2461 cagctcaacg gcgaaaacgt ccgccacgtc gaacaccgct acggcgatgc cggcaaagaa
     2521 agtacgatcg actacgtcag tgccgaactg ggcggcaagg cagccatcgt ctattacaag
     2581 acagacctca tgcacgatga aagcaatttc aacagccagg ccatgtacct cggccatgac
     2641 gaccaggtcg tcgacttctc ttactgggtt cccacacagg gcgtcaagac caatacggac
     2701 atcttgacga caggcgccct cctcgatacg tcgaagaagt tcttccgcgg tactatcgac
     2761 ttcctccgcg gcggcaagaa agccgtcggt tccgaatcgg atacgtgcat cctgctcagc
     2821 ccgaaggtcc attccatttc cgtaccgctc ctcctctgca aagaagacga cgtcgtcggg
     2881 aaccatgctt ccagtgccgg ccagatcgat aaggacaagc tcttctacct catgagccgc
     2941 ggctttaatg aaaccggcgc ccagctaatc atcgtcgaat cgaatatccg cccggtcatc
     3001 gacgccctgg gtgataaaga tttagaaaat aaagcgctcc aggctgtacg cgaaaagatg
     3061 cagttctgcc gcaagaaagg tgattgccat gctaaatgta cgcaaagatt tccccatctt
     3121 gactaaagcc cataatagac atcagatcgt ttactttgat aacgctgcga cgacgcagaa
     3181 accgtaccag gtcatccacg ccatcgtaga cctcttggaa caccacaacg gcaatcccca
     3241 tcgcggtgcc cacgtcctca gcatcgaagc cggtgaattg tacgccgcag cccgcgaagc
     3301 agtccgcaaa ttcatcaacg ccgcttcgac tgaagaagtc atctgcgtcc gcaatacgac
     3361 ggaagctctg aacctcgtag ctcgcagcta tgcagaaccg cgcctgaaga aaggcgacaa
     3421 gatcgtcatc cccatttcgg aacaccattc cgacctggtc acgtggcagc gcgtctgcca
     3481 gaagaccggc gccgtactcg attatatgta cctcgacgaa gaaggccact tcacagaaga
     3541 agacctggct aaaatcgacg acaagacgaa aatcgtcgcc tttgccgctg tcagcaacgt
     3601 cttcggcatg aagcgcccgg tcaaggaaat cgtcgccaaa gcccattccg tcggagccat
     3661 cgccatcgtc gacggtgctc agtcgacacc gcacatgaag accgacgtcc aggatatgga
     3721 ctgcgacttc ttcgccttct cgggccacaa gatgtgcggt tctgccagca cgggcgtcct
     3781 ttacggcaag aaagccatcc tggaaggcat ggaaccgttc ctcctcggcg gcgacatgat
     3841 cgaatatgtc aaggaacagt cgacgacctt taacgaactg cccttcaaat tcgaagccgg
     3901 ttcccagaac gtcgaaggtg ccgttgctct gaacgctgcc atcgactatt tgcaagacat
     3961 gggcatggac aacatcgaag
//
LOCUS       DAWCYS010000148         3979 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1231_length_3979_cov_5.580530, whole genome shotgun sequence.
ACCESSION   DAWCYS010000148 DAWCYS010000000
VERSION     DAWCYS010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_3979_cov_5.580530"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          961 a         1015 c         1038 g          965 t
ORIGIN      
        1 gggaaaacct atttgtcggc ctttgatgtc ttaaaaacgc atccccggcg gtttctcttt
       61 gtcgtccatc gggagctcat cgtcaaaagt gcacgggaca gttacgtccg gattggcatt
      121 aatccagccg atacggggct gctgacaggg cacgataaag aaatggataa gccctatatt
      181 tttgcgacca ttcagacctt ggcccaggat gaaatactcc atacctttgc gcccgatgct
      241 tttgactata ttgtcatcga cgaagtgcat catggcggag ccgcgacgta ccaaaaggtc
      301 attggctatt ttcggcccaa atttctgtta ggtatgacgg cgacaccgga acggtccgat
      361 gatttcgata tttatgcttt gttccatcat catattgctt atgaaatccg cttgcacgac
      421 gccttggaag aaaatatgct cgttcctttt cactaccacg gcatcagcga gattaccgtc
      481 aatggcaacg cgttggacga taaaagcgat tttgctctct tgacttgcga agaacgtata
      541 aaacatatat tatattatgc cgatttgtat ggcagtgatg ccgaccgcat caaaggcctc
      601 gtcttttgcc gcaatgtaga agaagcgcag gccttggctg aagcgtttcg ccagcatggg
      661 aaacgggcaa tcgccttgac gggcgcgtcg cgcgaaagcg aacggagtga agcgattcgt
      721 catttagagg cgaaagctgc cgaagacccg cagtatctcg attacatttt cacttgtgat
      781 attttcaatg aaggcgtcga cattccccag gtcaatcagg tcatcatgct gcgaccgaca
      841 acgtcggcta tcgtctttgt gcagcagtta ggccgggggc ttcgcaaata tccgcataag
      901 cgctatttag aagtgctcga tttcattggc aactatgaaa ataacttttt acttcccatc
      961 gccctgtttg gcgaccggac gtatgataag gattttgtcc gccgcctcat gcaggtcaat
     1021 tttctgccgg gaccgacgtc ggttcatttt gacgatatcg ccaaagaacg aatttacgcg
     1081 gctattgatg ccaaaagtgc tttggcagac ctgcgggatt tgaaagaatc atatcgtaat
     1141 atggcctatc gcctgggccg ccagcccatg atgatggatt ttgtccgctt tggcgacaag
     1201 gatccggcgc tgttcgtcgc taagaaagag tctttttttg aatttgttca atacatggag
     1261 ccttataact cgacgctgaa cgcatcgcat cgggctgtgc tgaagatgat gagcctggaa
     1321 ctggccaatg gcaaacggat agaagaattg ctggtcttgc gccatttgct gacagaagat
     1381 tcctggtcta cggcggccct ggctaaggaa atgaacgaaa cataccattt cctgccgtct
     1441 gcggaaacga tggaaagcgt ggcccgcctg ctggatttgc aattttttac taaaacggcc
     1501 agaaaaaaat acggcggaca gccgctcatt tcctttgaaa atcatgctta tacagcgact
     1561 ccttattgga acgatttgaa agaaaataaa gaatttcagt gctatgttca agatatcctc
     1621 gactatggga cctatcgttt tgaaagcctc tacgtccacg acgaaccgga aatcattaaa
     1681 ggctttgtcc gctacggccg ctattcccgt aaagacgtat cgcgtattct caattatgag
     1741 acgaatcgcg aaggcacgct caatggctat cagattgtcg gggctacgtg tcctattttc
     1801 gtcacctatg agaaacgtga cgacatttcg gccaatacga agtacgaaga ccagttcgtc
     1861 tcgccacagc agttcagctg gatgacgcgg gctcggattc acctgacgtc gtcgcagatt
     1921 ccggcgattt gcaatgagca cacgcggaaa ctgctgtttg taaaaaagag cgatgccgaa
     1981 ggctccgact tctattacat gggagatttg acggtattag gcgatcctgt cgaaacgacg
     2041 attgccgatg gcaaagggca gcagctgcct atcgtcaatt tccagttcct tttggacaag
     2101 cctgttgagg ataagctcta tcggtatctg tgtgagtgag tcattattta gtaaatcgta
     2161 aattagtaaa tcgtaactta ctaatttatg atttactagt gcaaggtgtt ataaacaggc
     2221 ctacacaaaa actttacacg ccacctgtgg tacattgacg ggaaatgtgg taaactagta
     2281 tagttataga agtatataca aaggggaggt gtttttttac tctatggaag ataaatatga
     2341 atcgaatcat gatgggccga aatcgaagaa gttcaatggc cgcaatgtgg ccgataacat
     2401 cataaaaggt gtcatcgtcc tgattatggc cgtcatcttc gtcatgattg ccgaccaggt
     2461 gattacgacg cccttcatca gtgccgaaat cgaaggggaa tttttccacg gcagtatttt
     2521 cggtacgact atcatcacca tcgtagccta tatcgtcggc atcatcgtcg gggccctggt
     2581 gggctatggg atttcgccgt tcgtgctgcg cctcatctgg gcagcgattc accgcatcga
     2641 atcgggcctg aatgatttcg agagccagga catcatcgtc gggacgctgg ggttgctttt
     2701 tggacttatc attgcgaatt taattggcct agcgttcgca cgcctgccga tcatcggggc
     2761 ctacgggcct atcgtcttca gcatcgtctt cggctacgcc ggcatgagca tcgccatccg
     2821 caagaagagc gaaatcatcg ccctggcagg caatctgcgc ctgggcaagt ccggcaagga
     2881 tcacggcagc aagcataagg acatggaatt ttccggcaag ctcctcgata cgagtgccat
     2941 catcgacggc cgcatcgccg agatttgcag taccggcttc ctggaagggc cgctgctggt
     3001 gccggtcttc gtcctggaag aattgcagct catcgctgat tcgtcggacc tgttgaagcg
     3061 caataagggc cgccgcggcc tggatatcct gaaacagatg caggaagata attacgtcga
     3121 agtccgcatc atcaacgacg atttcgacga cgtccagggc gtcgattcca agctcgtccg
     3181 cctgggccgc aagatcaatg ccaaagtcgt cactaacgac tataacctga ataaagtcgc
     3241 cgccctccag ggcgtggccg tcctcaacat caacgacctg gccaatgccc tgaaaccggc
     3301 ccgcatccct ggggaacaca tgaacgtcct catcgtcaag gccggcaagg aagagaacca
     3361 gggcgtcgcc tatctcgatg acggcacgat gatcgtcgtc gaagatggcc agaaatacat
     3421 cggttctact gtgcccgtta cggtgacgtc tgtcctgcag acgtcggctg ggcgcatgat
     3481 attcgttaag attgcaaatg agtaggtgaa atgtgtgaaa gtatctgtca tcgtcgtagc
     3541 ggccggcaca gggagccgct tcggctacga gcggaacaag ctcttctatc ccttgtgcgg
     3601 cgagcctgtc ctgacccata cgctgcgcca cattttcgcg gcccggtcgg tcagtgaagt
     3661 ggtcatcgtc cattcccgcg tcgatgagaa ggaaatccgc cgcctcgtcg atgacctcca
     3721 tcctatccag cccgtacgct acgccctggg cggcgccgaa cgggaagatt cggtctacaa
     3781 cggcctcatg gccgtcagcc gcgacgtgga tgtggtcatg gtccacgacg gcgcccggcc
     3841 ttttgccggc ccggactggt atgaccaggg cgcggccatg atggaaacgg cccgagctgc
     3901 catttacggc atccctctca aggatacggt caaggtccgc ggcgacgacg gccggctcca
     3961 tacgctgaac cgcagccgc
//
LOCUS       DAWCYS010000149         3947 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1240_length_3947_cov_5.577595, whole genome shotgun sequence.
ACCESSION   DAWCYS010000149 DAWCYS010000000
VERSION     DAWCYS010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_3947_cov_5.577595"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          789 a         1076 c         1058 g         1024 t
ORIGIN      
        1 cttctattca tcacgcttca ctttctctat ggccccggcg aagcctttgt catcaatgag
       61 gatgatgacg ctctgggctt tgccgacggc actgcctaat tcatttttcg tatatgtatc
      121 aacgacggtg atccctttcc gctccgccag gcggcgatat ttctcgacgt tatcgctgcc
      181 gccgtcggag gccaggaata agagcgggac gcgccggcgc ttcaatgttt tctgtgcggc
      241 tccatcaccg cagacggtct tgccagcccg gcaggcaagg ccgaggagat tcaggacctt
      301 gtcgctcaca gcagttcctt cctcagctgt tcatagattt cgctggacac tttggtcttc
      361 agggaccgtt ccaggcgatg ttccttgtag gctttttcca ggcattcgct gttcagacag
      421 agatagacgc cgcggccgga cttcttgccg gtcttgtcga tctcgacagc gccttccggc
      481 gtgcggacga cgcggatcat ttccttcttg ctcttctgtt cctgacagcc ggcacagacg
      541 cgcatgggag tctttcgggt cttcatggct tattctcctt ccgtatcggc tgcaacatcg
      601 tcagctgccg gcatggccag gtcgctgagg atgtcgatgt catcggccgg ttcactgtcc
      661 tgcggagctt ctgcctgggc agccagttcg gcagcctggc tttcgctctt gatgtcgatc
      721 ttccagttgg tcagtttcgc agccaggcgg gcgttctggc cggccttgcc gatggccagg
      781 gacagctggt aatccggtac gacgacgctc gacgattttt cgtcttcatc gaccgtgacc
      841 gaaatgacct gagccgggct gagggcgttg gcgatgtaga ccgccgggtc ttcatcccat
      901 ttgacgatgt cgatcttttc gttctgcaat tccgagacga tgttctggac gcgcgagcct
      961 ttcgggccga cgcaggcacc gacggaatcg acattggcat cgcgggaata gacggcaatc
     1021 ttagaacggc ggcccggttc gcgggcgacg gatttcagtt cgacgacgcc ttcgtagatt
     1081 tccggtactt ccagttcaaa gaggcgcttc aagaggcccg gatgggtccg ggatacgagg
     1141 atctgggcgc ctttcgtcgt cttgcggact tcgacgacat agcacttgat gcgctggccg
     1201 acgctgtacg tttccgacgg gatctgttcc gatgccggca ggatggcttc agccttgccc
     1261 aggtcgacgt agacgttgcg gttctcgatg cgctggatga tgcccgtgat gatgtcgtcc
     1321 gtgcggtccg agattgcatc atagacgatc gtgccgtccg cttctcgcag tcgctgaagc
     1381 atgacctgtt tggcagcctg ggcggcgatg aggccgaaat tgtccggcgt gacatcgacg
     1441 ttgacgacgt cgccgacttc gtaattgaca tcaatgaggc gggccttatc gagagagatt
     1501 tccgtatgcg ggtcttcgac ttcgtcgacg acggtcttgg cagccttgat ggcatagtcg
     1561 cccgtcgagc ggttcagttc gacttccgcc gtcggattgg catcgggttc cttcttgtaa
     1621 gccgtgatca ggacggcttc cagggagtca cagatgacat ccggagatac gcctttctct
     1681 ttggataagt aagcaatagc attgagcaat tctttattca cgttaataac ctccactaaa
     1741 aatcaatatg caaacggact tgcgatattt ctttcatgtc aatcttatct tcacggccat
     1801 cgacctggac gatgagatgc ccgtcagctg tcttttcgac gagatctgcc gtcaggtcct
     1861 tgacgccgtc gcgggcctta aagaaatgga tgtctacgga ccggccagca tagcggacga
     1921 agtccttgtc cttcttgagg atgcggtcca gtcccggcga cgatacttcc aggagatagt
     1981 tgtcgctgat cgggtccttt tcgtcgagga ccttgctcag cttctcgctg acggcctggc
     2041 agtcttccag gtcgacacca ccttctttat caatgaatac gcgcaggtac cagttccgtt
     2101 cgcgcacgta ttcgacgtcg accagttcca ggtcagtccc ttcgataatc tgttctactt
     2161 ccttggcaat caaatcttca atgtgttcac gtctcatcgt attcaccagc ttatcttcaa
     2221 aatttcctac aggtaaaagt taagagtggg ctttcaccca ctcttcgcat ctaaacatct
     2281 aaattacttt tagtatacca ctgtctgctc attctgacaa tgatttgtcc cggtcagccg
     2341 cgatttcgtc gaggacgggc aggaggcgtt tccgcaaggc tttctccagc tccttcaaag
     2401 acgacgtatc gaagtcgtgg accaggtgcg ggaagttctg cacccgccgt tcagcaaata
     2461 aatcgtcgcg caggacgttg tacatcaggg acaggccgct gcgcgtggct gcgtcgtaat
     2521 aagccaggat catgtaggac ccgttgatga tgcgcacggc atcgttgggc ttgaccaggc
     2581 gccggaagcc ctgatagctg tcgggcagga actcatccca tgcgacgtca gggacgccct
     2641 tgttcttgag gatgacgctg aaattgcggc tccgttcgac gtaatcgtcg tggatgatac
     2701 gggccaggcg ctcttcgacc atggaccgga agaggtcgaa ggaatcggtg atgaattcga
     2761 tgagggcgaa ttccagcatg ccgatatccg tccggataga gaattcttcc gattcgggat
     2821 tcaagatagc ccgcacggtc cagccgttga cgtgattctc ataggtgaac aagcggtact
     2881 cgacgggctt gccgtctttc tcgatcatgc gatattcttc caataactga aaatctccca
     2941 ctgaaagtgg gagaacagtt gtgatattcc attgtttgag tgtttcaatc aatgtagcat
     3001 ccatagtaaa aatggtgcgg ttggcgggag ttgaacccgc acgatcgaag atcactgccg
     3061 cctgaagaca gcgcgtctgc cattccgcca caaccgcata actcagaaat cgtaaagctg
     3121 tttgtaatgc ttgtcgttca gcacgactat tctaccatat tctcgcggtt ctgaaacggg
     3181 gagtttttca gtttcttgaa aatttttcgt ccagccatat ttctggatcc acgagtcgac
     3241 atgtccccgg ccggcattat acgcagccaa ggccagtact tgattcccgt cgtattcctt
     3301 caataagtag gccagatacc aggttcccaa cctgatgttt gtacggacgt cattgagttt
     3361 ttcaggcgtg tagtcggtca tccccatctc atcggcaatc cattgtgccg tgtcgggcat
     3421 gacctgcatg aggccatggg caccgggctg ggaagcagct cgttcgtcaa acttgctttc
     3481 cactaagatg acggacgcca ccagggcggg gtcgactttg tcttcgtagg catactgccg
     3541 aaccaggtaa tcgtactgga gagggtatac gaacttccgc atgacatcat ctttccaaga
     3601 ataaaagtac gatgcggaca gacatatcaa taatgccgct atgatgattt tccctttggt
     3661 gcttgctctt tttctcaacg tcttctccct accctttgat gcggtccatc aaggcctgcc
     3721 atgcgacgag tacctgtttc tgcgtctctt ccggagagcc ggtattgtcg atgatgacct
     3781 gtgccaaaga ccgtttgtcg tcgataggaa gctgtgcatg aatccgtgcc agtgcttcac
     3841 tttcgggata gccgttccgc ttcatcagcc gcgtaagctg cgttaccgga tcgacgtaca
     3901 ccagccacgt ttcgtcaacg tacgaatcgt attttacctc ataaagc
//
LOCUS       DAWCYS010000150         3937 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1244_length_3937_cov_4.500773, whole genome shotgun sequence.
ACCESSION   DAWCYS010000150 DAWCYS010000000
VERSION     DAWCYS010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_3937_cov_4.500773"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          834 a         1190 c         1116 g          797 t
ORIGIN      
        1 cggcccaggc gacggccctg gttgccggcg acatgaccat gccgggccag cagccctatc
       61 ctatgggtga actgaccttt gacggtctcg tcgacgtcta tcgcgaacag gcccaggcct
      121 tgtacgatgc cggtgtcgac ctgttcatcg tcgaaaccat gatgagcctc caggaaacgc
      181 gggcttgtgt cctggctatc aaggaggtct gcgacctgcc catcatggcg acactgacct
      241 ttgaaaaaga cggccgtacc ctctatggga cgccgccgga agcggctgtc gtcgtcttgc
      301 agaacctcgg tgtcgatgcc gtcggcctga actgctcgac aggaccggcc gacatggtgg
      361 aaacggtccg gaagatgtat gaatatgcga cgattcccct cattgccaag cccaatgccg
      421 gtttgccgga attggatggt gacaagacgg tctataagac gacgccggaa gaattcgccc
      481 aggacggccg gctgctcatc gaagccggtg cccatatcgt cggcggctgc tgcggcacga
      541 cgccggacca tatccgggcc cttcacgagg ctgtctgccg gctgacgccg aaagcgccgc
      601 atgccagcca tcaccgcgtc ctggcctcgg aacggcaggt cgtagaaatc aagctcgacg
      661 gctctttcca ggtcattggc gaacgcatca atccgacggg caagaagaag ctccaggctg
      721 aactgcgggc gggcaagctg gacatggtcc gcgccatggc ccgcgaacag gaacgcaacg
      781 gcgcgtctat cctcgacgtc aacatgggga ccaacggcat cgatgaaaaa gaaatgatgc
      841 tcaaggccat ttacgaagtg acaggcgtat cgggcctgcc cctcagcatc gacacgagct
      901 caccggacat catggaagcg gccctgcgca tctatcccgg ccgggccctg gtcaattcca
      961 tttcctgtga aacggaaaag cgaaagaaac tcctacctgt cgtcaaaaag tacggcgcca
     1021 tgttcatcgc cctgcctgtt tcggataacg gtatccccaa gaccctcgaa gaaaaacatg
     1081 ccgtcctcga cgagctcctg gccgatgcct acgaccacgg cttttcgccg gaagatgtcg
     1141 tcgtcgacgc cctggtagcg acggtcgggg ctgcgccgac gtcggccctg gactgcatgg
     1201 ccacgttttc tcactgcaag caggacctgg gactggctac ggtctgcggc ctgtccaaca
     1261 tctccttcgg cctgccggac cggatctatg tcaacgccac cttcttgggc atggccattg
     1321 ccagcggcct gaccatggcc attgccaatc cgtcccagga tttgctcatg aatacggcag
     1381 cagcggccaa tatgctcatg aataaagagg gcagcgattt ggcctacatc caccgcatgc
     1441 agtatttcga caagaaagaa gccgaaagaa aacggcagga agaagcagaa ctcctggcca
     1501 gccacggtac ggctggggct gtcgaaaagg cgggggagtc ccaagacgct gcgcaagggc
     1561 cgctcaatcc cgtctaccac gctgtccgga aaggggaaaa ggacgccgtc gtcgccttgg
     1621 ctcaggaaga actggccaag ggtatggcac cggatgccat catcaacgac ctgctcattc
     1681 cggccatcac tgaggtcggc gatttatacg ataaacagat ttacttcctg ccccagctca
     1741 ttgccagtgc caatacgatg gaaacggcca ttgcctattt ggaaccgctc atcaagaaag
     1801 acccgggcca gcaggacatg gcgaccatcg tcattgccac cgtcgaaggc gacgtccacg
     1861 atatcggtaa gaacctggtc ggcctcatgc tgcgcaacta cggctataag gtcatcgacc
     1921 tgggcaagga tgtgccggcc ggcaccatca tcgagacggc gctgcgcgaa aaggcatctg
     1981 tcgtcggctt gtcggccttg atgacgacga cgatgatgca catgaaagat gtcatcgccg
     2041 aagcggcgga taaacagtat ggcggcaaga ttatcatcgg cggcgcagcc gttacgccga
     2101 gtttctccga tgaaatcggt gccgatggct attccaaaga tgcggcagac tgtgtcaagc
     2161 tcgtcagccg cctgttatcg taaaaggggg aatcattatg ccagcatatc gttgggcgac
     2221 tttgggctgc ggcgtcatcg gcaatgaact ggcccaggct atgcaggccc tgggcgggaa
     2281 tttgtacagt gtcggcaacc ggacccatga aaaggccttg tcttttgctg aaaaatacgg
     2341 catcgccaag gtctacgaca agccagagga tatcttttcg gatcccgacg tcgatatcgt
     2401 ctatattttg acgcctcata atacgcatat ccgttatctg ctgccggcgc tggcagcggg
     2461 caaacacgtg ctctgcgaaa aatccattac ccttaacagc ctggaactgg accaggcccg
     2521 gcgctgtgct gatgaacacg gcgtcgtcct ggccgaagcc atgaccttat atcatatgcc
     2581 tatttacaaa cagctcgctg aactcatccg ttccggcgcc ctggggccgc tgcgtttcat
     2641 tcagatgaat ttcggcagtt ataaagagta cgatatgaac aaccgcttct tcaaccgctc
     2701 cctggccggt ggtgccctcc tcgatatcgg cgtctatgcg ctgtccttcg tccgctattt
     2761 catgagctgt cagcccgacc agatcgcgtc ccaggtccgc ctggcaccga ccggtgtcga
     2821 cgagcaggca ggtatcctct tgcagaaccg ggaaggggag atggcgacgc tttccttatc
     2881 gctccatgcc aagcagccga aacggggcat ggccgccttc gataagggat ctatcgaact
     2941 ctacgacaat cgccgggccg atacggcgac catcacctat acggccgacg gccatcagga
     3001 tgtcctgcag gccggcagtc agtccaaggc cttgcagtac gaaatccggg acatggaaga
     3061 cgccgtggcc cacggcggcc agggcatgtg ccaggcttat acgaaagatg tcatggacct
     3121 catgacggcc atccgccagc agtggggcct gacctatccc gaagaagagg ggggagcgca
     3181 atgagttatt catcggtcga taccttgtgg atcctcatgg cggccgtcct ggtcttcttc
     3241 atgcagcccg gtttcgccat gctcgaagcc ggcctgacgc ggggcaagaa tgccggcaat
     3301 atcgtcatga agaatttcat gaacttttcc ctcgggaccc tggccttctg gatcgtcggc
     3361 ttcagcctgc tctttggccg cgacataggc ggattcatcg gcatgcccga tttcttcgcc
     3421 cagcatatgg acctcatggc cgggacgcct tatccggatt tggccttttt gctcttccag
     3481 accgttctct gtgccgtgac ggcggccatc gtgtccggcg ctatggctga acggacgaaa
     3541 ttttcgtcat cctgtctgat tacgatcctc atcagtctgc tcatctatcc catcgtcggg
     3601 cactggtcct ggggcggcgg ctggctggca gccctgggct tccatgactt cgccggcgct
     3661 tctgtcgtca gcctgaccgg cggcatctgc gccctgaccg gggccaaggc cctggggccg
     3721 cgcatcggca aatataatga cgatggctcg gtcaatgcca ttcccggcca cagcctgccc
     3781 ctggcttgtc tgggcatgtt catcttatgg ttcggctggt tcggcttcaa tggcggccag
     3841 accctgtccc tgacgggaga acagaccatc ctaaaagccg cgtctatctt ctatacgaac
     3901 aatatagcct actctgccct gactactacg tccatgt
//
LOCUS       DAWCYS010000151         3916 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1247_length_3916_cov_4.616680, whole genome shotgun sequence.
ACCESSION   DAWCYS010000151 DAWCYS010000000
VERSION     DAWCYS010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1247_length_3916_cov_4.616680"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          985 a         1021 c          987 g          923 t
ORIGIN      
        1 tatcagtatt acatgtctag gtggggtgtg acgcttgatg aattcataag tagtgtgtgt
       61 ggtattaaag atggcttttc catatacgat aagggcaaca atctgtatgt cgtgtcttac
      121 aatacccagg atatgacgca ggcccgcatc cgctggacgc tggcccatga actcggtcat
      181 atcatactca accacttcgg cgagttcgat gcaaccagtc tttgtggtcc gggacttacg
      241 gaagaggaat acggtgtcct ggacgtcgag gccaacacct ttgcttcaga gttactgtgt
      301 tcccccgcga tggtcggtct gttgccggaa tgggagcgga atgccgatgc ggtcagccga
      361 tttttcttcg tgtccaaaca ggccgcggaa aaagccatcg caaagtggga cagctaccca
      421 ggcttgcata gcgctcacgc agcttttttc aagagccagt tcccttcgta catcagtcac
      481 gaatccgctt aatcgaatcg aggtgttctc aagatgaatg taaaacggac gatcgtgacg
      541 ttgctgttac tgctaaccat ggccggcgcc gcttctgcgg cagagccttc gtggtactgg
      601 ctatgcagtg atgcgacatc aacaacgtat ctggataaca gccatgtgta caagaatgac
      661 gattgcgcta tcgtgtggac ccggaccaat ttccagtccg gggctatggc cggcgacgtc
      721 gtttacatgg agtggcacgt aacgcgcgcc ggtgagattt cgtacatcta cggtcatggc
      781 cataaagcaa atggccacct cacacggcac tacccagacg catactattg gaataaagcg
      841 gtatcaagcg cgccgaattt caagaaactc tataatctga tttggcccgc ttagaaataa
      901 tggaaagaag ggattcatat gttctgtaca aaatgtggta agaagatcca ggacgacgcg
      961 aagttctgtc cgtaatgcgg cgcgacagtg gagccggtcg tcgccctgag ccctccgcaa
     1021 cgtccagaga ggcgtcagac accggtaaca aagcccaccg ctcccccgcc gcctgttcct
     1081 ccgaagaaga gcaagaaagg tttttggggc gctctcgtcg tcatcctggc tatcctcgga
     1141 ctgctgtaca ctataggaac cggcaaccag cagggaaaga aaatgaacac aactcccacc
     1201 cctgccgcga ctacgcagaa gcagcaggcc gcgcagaaga agcccgcgcc ggggtccatc
     1261 gagttcttcg aggtggataa ggacgcaatc ggccagcctg tcgctttaat cccgctgaac
     1321 aacgagagcg acaagacggt agatggcttc aaggtcatcc tgtcggcgaa ggataatttc
     1381 gggaatccgg tcaagcagtt cggctacggc gacccggtca tgaagctgat gagccagcgc
     1441 acggtaggcc cgcacgaagt ctctacgacg gaccactact ggcacttata tggctttgaa
     1501 aatggcacga agttccatat caagctgcac gaaatccatt acactgacgg cacatcgtgg
     1561 aaggccgaag aaagtgagcc tgtagaagcg gataccacga agaccgataa agtagtccaa
     1621 taaggagcgt gattatcatg acaatcgata tggtcctcat cctattgggc ctggccttga
     1681 ttccggcctt tatcgcaagc agcaagggcc gcagcttcgc gctgtggtac atctatggcg
     1741 tctgcctgct catcgtggcc atcccgcact cgatcatcat cagtaagacc actgagcaga
     1801 aagataaaga gctgaaaagc gcaggttacg tagaatgtcc gtactgcaag gaacccgtga
     1861 aggctggcgc tgtcgtctgc ccgcactgcc gtcgtgagct cccgcagccg gattctcagg
     1921 ccgttgaaga ccaggtgaag tgccggacct gcggtatgat ggtcaaaaaa ggcgcgaagt
     1981 actgcccccg atgtggcaat ctgatcgatt aaagacaaaa aaatatcccc ggatgaccgc
     2041 tactcaccgg ggatatttag gagctgaagt gcgatatgaa tgaccgcaac ctcatttgca
     2101 ttatagcaca tcagccctat tatctcaaga aaatctataa ggagctgatg aatatgtatg
     2161 tacgtaaaat taataaccgt tggtactaca ctatcgaaga taaggacgcc cagggcaacc
     2221 gtaaacgcca tgaacgcttc ggcggctata cccgggctga agccgaaaaa tcttatcggc
     2281 aggccatggc cgaaaaggac cgtacaggca aacatttcga accatccgac atgtcttttg
     2341 atgagttcct ccaggaatgg cttgataagt atgtccagaa ttatttgaag ccggccaccg
     2401 tggacgcgta tagtgcggcc atccgtaacc acatctcccc cactttcggt acgcgaaagc
     2461 tgcgggattt gacgacggct gctttccagg actggcttat cgagctgacg agtaagtata
     2521 caaagtctac gctcaaagtc atcatgagtt gtctccgctc cgcgctccgt tgggctgtcg
     2581 ccaatcgccg ctacctcttg atcaacccga tggataacgt cacgctgcca ccctgccgga
     2641 cgctgccgaa atcgccgaac atcttcacgc cggaagccat ccaggtgatt ttcaaaaaat
     2701 tcccgtcggg ccatcccatt catatgccat gcgtcctttc ctactacacc gggatgcgcc
     2761 tgggtgaatg cctggcttta gagtggtgca acgtcgacat gacggcgcgc acgatccgcg
     2821 tcgtcggcac cgcctatgac catgccgggc ccacaaagat tttgacgccg aaaaccgctt
     2881 cttctgtccg cgtcatcacg ttcggatcta aattgcaagc tgagctgaaa gcgcagctgc
     2941 tctggcagaa tacgaaccgt ttccattacg gagcgtttta taaagaaggg acggagcacc
     3001 atttcgtctg caccatgccc gatggccggc aaatgaccag caacaacacg aagtatttca
     3061 acatgtggtg taaagaagac ttccccggca cgagcttcca ctccttccgg cacacccacg
     3121 ctaccatgct gcttgagcat gggtttaaga tggactacgt ctcgaagcgc ctcggacact
     3181 cctccatcta caccacggcc aacatctacg acaccattac cgacaaacgc gaaaaagacg
     3241 ccgtcgatgc tatggaggct ttcttgtaat gcataattat tttcttaccg ttcttccgcc
     3301 gcgggaagcc aatggcggaa gaaatggcgg aaaaacatcc gcaaaccgcg ccatgactgg
     3361 ctcttgcttt aacgttaaat tatcggcagt aataaaatgg gagattctgg gagcatctgt
     3421 aaagctttat gaacggcttt attaagccaa ttatgaggta tcatgtcgca ttgtcaatat
     3481 tcttcaaatc ttgtcaaatt cccttaattt tcatttcaaa gtgggaagtt ttgtgggaag
     3541 tttttgtggg atgttttact tgctgaagcg cctaaatttt tttgtcaatg tgggaagttg
     3601 tgtgggaaat tgtgcgaata gcatcttgga acaataccac ggtcaattat ctacggtggt
     3661 attttaatta ttatactaaa tctacagact ccagccacgg tacatttctt cttcgactcg
     3721 ttccagttca tctttctttt tgtgatgatc ttcatcatcc caccatgcct ggtatcggcc
     3781 gtgagtatct tcgactgctt tttccaggaa gtggttgatt tttcccaaga cttacgtctg
     3841 gcgctgctga tggacggcct ctgtatgtgg cggatagtga atgccttctc ctcgcgtgag
     3901 aattctcttc ttggca
//
LOCUS       DAWCYS010000152         3903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1252_length_3903_cov_6.996102, whole genome shotgun sequence.
ACCESSION   DAWCYS010000152 DAWCYS010000000
VERSION     DAWCYS010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_3903_cov_6.996102"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          887 a         1167 c         1076 g          773 t
ORIGIN      
        1 tgaaaatggc ggccagatcc gcgatttcat ctacgtcaac gacgtagtca acgtcatctt
       61 ctatttctgg gaacatccgg aactgtcagg catctacaac tgcggcaccg gcatcggcca
      121 tacgttcaat gaattcgtcc agggcgtcat cgattattgc ggcaaaggcc atatcgaata
      181 cgtccccttc ccggacatcc tcaagggcaa gtaccagagt tatacccagg ccgacacgac
      241 gaagctcatg gaagccggct atgaccgcgg ctttaccctg ctcccggaag ccgtcaagga
      301 atactgcagc atcctggaaa agagcgacgg gtattacgaa tgaggaaggc cgtctttttc
      361 gaccgcgacg gcgtcctcaa cgtcgacaaa tcctatttgg gcttcatcaa ggattttgaa
      421 tggatggacg gggccaaaga ggccctggcc tatctgacgg aagagggcta tgccatcatc
      481 gtcgtcacca accagagcgg cgtcgcccgc ggttattata cggaagacga cgtgaaggtc
      541 ctccacgact ggatgtgccg ggaagcggcc aaagccggcg gcatcattac ggccgtctac
      601 tactgccctt atcttgaagg ggcgccggtc aaggcctatg acaagaagag cgactggcgc
      661 aagccggcgc cgggcatggt cctgcaggcg gcgaaagatt acgatatcga tttgaaacac
      721 tctttcatga tcggcgatat gccccgcgat gtggaatgtg ggcagcgggc cggcatggat
      781 ggctatttat ttactggcgg caggctggac gatttcgtcc gggccatcgt cgcagaaagg
      841 aagggccgtg aaagcatata agaacatact ggtcatcaag atgagctctc tcggcgacgt
      901 ccttcacgcc ctgccgacac tctacgcatt gcgtcagaac tgcccggacg cccgcatcgt
      961 ctgggccgtc catgaacagt tcgcgccggt cctgccggga aaaccttata tcgatgaagt
     1021 catctatatc gacaagaagc gactgaaatc cctgtcgtac tggaaagagc tgcgccggac
     1081 gctccatgcc tatcatttcg acgtatcctt tgacctccag ggcctggcca agagcgctat
     1141 cgtcgcctgg ttgagcggtg ctgaagaacg ctatggctac tgggaaatgc gcgaaggcag
     1201 tttcctggtc agcaagggcc tcacgggtcc ccacaagtac gaccacgtca tcgaacgcta
     1261 tctcgatacg gtccgcgtcc tcggcggccg cgtcgatgcc atcgaattcc cgcttcccga
     1321 tattaccggt gaaagacggg cctggcgcca gcgtctggcg gcggacggcc tgaccggacc
     1381 ctatgtggcc atcgtgcccg gtgcccgctg gagcgtcaag gaatggccct tgtcccattg
     1441 gcaggaattc ctgcagcgcc tgacggcgtc cggcatggcc gccgtcctct taggcagcgg
     1501 cgacgacgtc cccaaaggga aggccctgaa ggaccatgat tcgtcgggcc aggtcttcga
     1561 ctatacaggc cgcaccgatt tgcggcagct catggcggcc attgctgaaa gcgccctcta
     1621 tatcagtgcc gatacggggc cgctgcacat ggccaatgcc ttgaaaaaag aactcatcgc
     1681 cctctttggg ccgacgtgtc cccagcggac cggcccttac ggcggaaacg acgacgccta
     1741 catccacatg gtcctgtcgc cgacgtccca ggcgacgccg gaacggccct tggtcgatga
     1801 tccggactgc atggcccaga ttacgcccga tgcgctgtgg caggtctatg aaggcgtcct
     1861 gaagaaggtg gaactatgat taatctgcgc aacaaaaaaa taatcgtcac ctttctcatg
     1921 catctaggcg acctcatcct catcaccccg ttcctgcagg tcctgcgccg tcatgcccag
     1981 ggctcggaaa ttaccctcgt cgtcgatgag aaagtggccg acgtcgtccg ctacaacccc
     2041 aatatcgacc acctggtcac ggtcgacaag aagggtaaag acaattccgt ccgggccctg
     2101 tggcgtatcg ggcggcactt gcatcagcat cactacgata tcctcatcaa tctgcatccc
     2161 aatgaacgga cgtccttcct ggcggccgtc atccacgcca agcagttcgt cggcatgagc
     2221 catttcctgg tccggcccct gatggaccgc tatacccgcc tggaccgtat ccatctccac
     2281 gcggccgata tgtacatcaa cgtcctggcc cagctgggca tcgacgacta ccgcagcgac
     2341 ggcctgcagt tcttcacctg caaggcctgg gatgacaagg cgacggaatt ctaccgtacc
     2401 cagggcgtcc acaacagcga tgccctcatc ggcttcaata tcggcagcgc cgtcccgcag
     2461 aaacgctggc cggccaagcg ctttgccgcc gttgccgact acttcgcccg ccgcggtttc
     2521 aagtgcgtct tctttggcgg acccatggat gaagccatgg tcaaagaagc gacgtcccag
     2581 atggaatcgg acccgatcat tgcccacggg aacttccccc taggcgaact ggcctcggcc
     2641 ctccgctgat gctcgcggtt catcaccatc gccaggggcc atatgcacgt cgctgtcagc
     2701 cagggcgttc ccctggtcgc cctctacggg ccgagcaatc ccaagcttta cggcccctat
     2761 acggaccggg ccatcgtcct ggaatcgacg aaccactatg aagttggcaa gagcatgaaa
     2821 caaattatcc gcgaaggtaa ttataaaggt atttcggtta ttcccatgag ccaggtcatc
     2881 gccgccggcg aagaactcct gagccgttat tatggaagaa aataacccgt attatatgga
     2941 ggtggaaggt tatgaaaaaa cttattattg cagcgaccct ggccgccctg gccatgactt
     3001 cggctgctat ggcagccccg ttgcgggatc ctcagcccgg tgacttgaag gccaacgcca
     3061 actacggctt cgaccagaaa gaaggcggcc gcagtgccaa gagccgcctg accggtggcg
     3121 acgtcaccta cgtcctgaac aatcgctggg acatccagta cgtcaacaac tacaccaagg
     3181 gcgataacga caacaagatc aacgaaaact atctcgtcgg taattaccgc tttacgccgt
     3241 atctgtcggc ctttgccggc ggctcctacg tcaagaccga aacgtataat acgaccaagt
     3301 cctatggcta tcaggtcggc ctcaaaggcc agctccctct ggcagcccgc tggcagggct
     3361 ttgcatccgt cggtgtcggc gatgacgtca atacctatga agtcggcgtc ggttacgaca
     3421 tcacgccgaa ctgggatgcc cacataaaat accgcagcag cagcgtcgac gtcgataact
     3481 acgacgacga tgtcaaaggc tggcaggtcg gcatgggcta taagttctga tattgacaag
     3541 gggcggatcc gtcccgtata atgtaccaat gacatgtgct gtggttgaaa gtcgatgtca
     3601 gtcgcagacc aagcgatcca cgtaaggcac ccaaagcggt gctgatcata gtgcggccta
     3661 gaagtaagac ctgccgcgga aatactgcga gagggctagt agtgggggac agcagtctat
     3721 gagcgaacgt cccagcaggc gagtgtgggg gtaaagacca ggtcaggcat gtcagggaaa
     3781 ggggctgtcg tatgaaggat tctgccatca tgcgcagtcc ctttttcttt atagtttgta
     3841 gaagatgtcc ataaatatca aaccatccgc taaccactag ccgctagacc ctagccccga
     3901 tcc
//
LOCUS       DAWCYS010000153         3900 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1254_length_3900_cov_5.232250, whole genome shotgun sequence.
ACCESSION   DAWCYS010000153 DAWCYS010000000
VERSION     DAWCYS010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_3900_cov_5.232250"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          916 a         1113 c          986 g          885 t
ORIGIN      
        1 cagtgtgacg gcgccgatgg ggaccgacag ggaatggaag gcttctccgt cgcggactaa
       61 ggtcatttcg acgctggcac cgcccaggtc gaagatgagg aagtccttca tggcgatggt
      121 gttgatgacg ccgagatagc ctaagtacgc ttcccgttca ccggtgatga cgttcatggt
      181 gatgcccgtt tcggcattga tgcgcttcag gaaagcttcg ccgttgctgg cattgcggac
      241 ggctgccgtc gcgacggccc ggcattcatg gatgtccatg acttccatga tgtgcttata
      301 gacatgcagg caggtcaggg cccgctgcat cccttcttca ctgatgacgc cggtctggct
      361 caggccgtgg cccaggcgga tggcttcttt ttcctgatag agcagggaat aagactggtt
      421 gtcggcaatc tgcatgacga caaaacggat ggagttagag cccatgtcga tgatggctaa
      481 tttttccatg gtatacctcg tttctgcgga tgtttatcaa gtttatcgtt taccttttcc
      541 attatactac agaatagaga ggcatgccga aaatttttac aaaaaatata catacatacg
      601 gtgactttca tatataataa gaagagtatc agctagacag caactatacc gtcaagaaaa
      661 gtggggagag taatggaaaa acaaacccgg aatgttttcg gtatcatcca tatcgggacg
      721 gtcaatatga ccttgaaggt cattgcttac tcgtctttgg atgatatgga aatcatcgaa
      781 aatgtatccc gagaagtaaa atatggggaa acggtattcc agcgccatca cgtcagtttt
      841 cagtccctga acgaaatctg tcaggtcctg ctgggattcc gccagctcct ttgcgactat
      901 gatgtcaaag atgtccgcgt cctgtcgacg acagtcatca gcgaagccga caacttgatc
      961 agtgtccttg accagatcca tatccgtacg ggcttcgacg tcgaagtcat ccacatgacc
     1021 aaggaaatct attataagtt ctttggtctt tattataatg tattgaaagg caaattcaat
     1081 tttgccgata aagccgtcat gctcctggac atcacgtctg gcggcgtcgg cctgacctgc
     1141 tggaaatcgg accgcctctt attccagcag aatatccaca tcggctccct gcgcatcctg
     1201 gaaaatttta cggaaaaaca gcgtgacgag ctgacgttcc cgacggctgt gcgcgaatat
     1261 atctacggca ccctttatcc gctctggaaa ggcgtccagc agtatgatat caagtatatc
     1321 gtcctgtctg gccgcggagc caacttggtg gcacgcctca tgggcaagac cagtgaaaac
     1381 ggcctcttcc tgatcacgcc ggatgaactg cgccagttcg tctattcctt ccacggcgtc
     1441 acgtcgttca agctcatgaa gcgcttcggc ctgtcccaga acctggccaa tgtcatcatg
     1501 ccgacgattc tcttgtatta tgaactgctg cgcatcgtcg atatcgacat gctggtcgtc
     1561 atgccgacga cctttactga aggctatacc atgtattatg tggccgaaaa gacccataac
     1621 gattatatgg cccatgtcca gggcctgctc ctggacatga tgcgccgcct ggccaagaag
     1681 tacttgtccg attccatcca ttccgcccgc atcgaagaat actgcaacgt cctcttccag
     1741 gcgctgtacc agttcatcgg cctcgattac agctataatt atctgctccg cctggccgcg
     1801 attttccacg aaacggggaa attcatcaat atccgcaaac acaacctctg cacgtataac
     1861 ctcatcaggg aaacggacct cttcggcctg gccgacgatg aaaaggaaat cctggccgcc
     1921 gtcctctact atgcctttga cggtaccttg caggaagcgt cgccagtcta ccattccctg
     1981 acgccacggc agaaggtcat tacggctaag ctgacggcga ttttccgcct ggccgattcc
     2041 ctggacaaga gccatttagg gaaaatcagc gccatccggg cggaactcag ggaagacgag
     2101 ctcgtcgtcc attatacggc cgatgccgat atttctctgg aacgctggac atacctgaaa
     2161 atggccgtcg actttgccga agtctttggc atttcgacga aattagtgaa aggataagat
     2221 aatcatggca gattttacgg atgctcaata ttttttaaat cgtgaatgta catgggtcaa
     2281 attcaatgaa cgggtcctgc gcgaagccat cgtcccgtcc aacccgctgc tggaacagct
     2341 gaatttcatc gccatcgcag cgtcgaacct ggatgaattc ttcatgatcc gcgtcgccgg
     2401 tgtcaagcac ctcgtcgaaa gcggggtcaa gcacatcgac atcgccggca tgacgccgca
     2461 ggaacagttt gaagccatcc agtccatggt ccataagctg gtcggcgacc agtaccgcta
     2521 ctatggagat atccagcaga agctgcaagc tcacggcctg cacttcatcg gcgtcgatgc
     2581 ccttgacgat gaccagcggc tgtggctgga aagcttcttt gaacgggaaa tctacccggt
     2641 cgtcacgccc atggccgtcg attccagcca tcccttccca ttcctgtcca gcctgaacct
     2701 gaacctggcc gtcctcttgc gccgcaaaca gggggaccgc tcggtcaaga cggctatcct
     2761 gccggtcccg atgtcagtca tcgaccgcat catcaaggtc ccggacagtg tttcgccggc
     2821 tggcaagcag catgaaggtc atcagttcct cttcctggaa gacgtcatcg ccgcttatgc
     2881 ggaacgcttc ttcctgggct gcgacatcct ggaagtcgaa ccgttccgcg tcacccgaga
     2941 cgccgacctg gatatcgacg acgatgccga agacctcctg atggaagtcg aaaagagcct
     3001 gaagaaacgc cgcaaagggg cggctgtccg cctggaactg gctgcttcgt cgtcgcgcat
     3061 gatccgcgac ttcctgcgcg atgaactgca gctcgaagaa cgggacatct attcgattcc
     3121 gggtcccctg gactgcaagg tcttcttctc cttcgtaggc atcgacggct acgatgacct
     3181 gcgctatccg ccggtgacgc cgcagccgtc gtcggaactg gccgccatcg acgcgcctga
     3241 tttctggtcg gccattgccg aacgggacat catagtccat catccctatg aaacctttga
     3301 aacggtagaa cacttcatcc agctggctgc gacggacccc catgtcctgg ccatcaaaca
     3361 gacgctctac cgcgtcagca gcaagtcgcc catcatcgtc gccctggccc aggccgccga
     3421 aaacggcaaa caggtcacgg tcctcatgga agtcaaggcc cgtttcgatg aagaaaataa
     3481 tatccacatg gcccgcaagc tggaaaaagc cggctgccat gtcatttacg gcatcatggg
     3541 cctcaagacc cattctaaga tcaccctcgt cgtccgccgc gaagaagacg gcatccgccg
     3601 ctatgtccac ttggcgacgg gcaactacaa cggcaagacg gcccgcatct atacggacgt
     3661 cggcatcctg acggccaata ccctcatcgg cgtcgacgca tcggctttct tcaacctctt
     3721 gtcgggctac tccgatcctc ctcagtggaa taagctggcc gttgcgccgc tcaacctgcg
     3781 ggaaacgatt tatcaggaaa tcgatcagga aatcgctcag gccaaggccg gcaagaaagc
     3841 cgtcatcgtg gccaagatga attcgctcct cgataagtac gtcatcgcca agctctatga
//
LOCUS       DAWCYS010000154         3849 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1273_length_3849_cov_5.762256, whole genome shotgun sequence.
ACCESSION   DAWCYS010000154 DAWCYS010000000
VERSION     DAWCYS010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_3849_cov_5.762256"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          951 a          903 c          968 g         1027 t
ORIGIN      
        1 aagccaaaac atcagctaaa tttttacggt caccaatttc tacattaatg ccaacatttg
       61 catttgaaac ttcggcagct aaatatgagt ctttttcaac gatggcaata acagcttttc
      121 ccccttgtaa ataactataa tacttgctag gtgcacaggt tccttttaat cctttttcta
      181 agctgacaat actgacagaa ctaatcgaaa cagcttgttc aaaatctttt ccaactagga
      241 aatccaagac cttcacatga ggcatatttc ggacagattt ttttacaaat tccattttgc
      301 taccatgccc cacaatcaaa tattgaatcg ccgggttgtc ctttgttaat tggatagcac
      361 ctaataacgt attcatatcc tggcaaattc ccatattacc aaaatacgaa acaataaact
      421 gtccattggg ataaccaaag tgctgatagg cgttaggcgt tacgggaatc ttcgtttcgt
      481 gtgcccaatt agcaatggta caaatacgat tctcgctcaa ttggggccga tgctttagta
      541 aaaagccttt catttcatca gtcaaggcaa ctaccataga agcttttgca tataaaaaac
      601 cattaatccg cttcatgata aaatcaatca ggcttcctgg ttttagactt ttagatgcat
      661 aagctacttc aggataaaca tcataggaaa caaaaacaac tttcgtctta aacagaaaat
      721 ttgccagcaa agccacacaa ggtaaaatcg gtggatttga atacacaata attattttat
      781 attttcttag ctgagttaca ttgagcaaag caccaaaagt aaaagaaaaa taattaatca
      841 agcgccccca aaaagaacat ctggacactt caacatattt aattctttta atgccaatgt
      901 tctttatcgt ttcgcgtaat ggtacatttt tttctgctgt gtattcttta ggataaccgc
      961 ataatgcgtc tacctgtaac ccatgctcag ctaaataaac agccgtatca aagggtaatg
     1021 tcgccgaaga gttatgttca ggataaaaaa attggcataa aaacaagata tcttttttct
     1081 tcatagcagt gctttttacc tttcatgatg atctatcaaa cttttttcag attctctgaa
     1141 aagttcccat gcgggccacc tcccctatta ggggcgctgg aatttgctta ggtccaggtc
     1201 tttgccgctg acgtctctgc cttcggtggc gctcttggtg actaggatct tgatggtttg
     1261 gatcatgatg atgaagtctt cccagatact gtagttttcg atgtacatca aatcgtagac
     1321 gagtttgtca taaggcgtgg tgttatactt ggcgaagacc tgggccaggc cggtgatgcc
     1381 cggtttgacg ttatggcgat aggcgtattc gggattttct tgtttgaatt gttcgacgat
     1441 gaaaggccgt tccgggcggg gcccgacgag gctcatgtct ccgttgagga cgttgaggat
     1501 ctgcggcagt tcgtccaggc gggtggcacg gatgaatttg ccgactttgg taatacgcgg
     1561 gtcgtcttcc gtcgccagct ggggaccgga atatttttcc gcatcgacgc gcatggaacg
     1621 gaatttatag actttatatt ccttgccgaa gcggccgaca cggacctggc tgtagaggac
     1681 gggaccaccg tcgccgagtt tgatggccag agcgacggga atggcgaagg gcaaggcaca
     1741 gatggcggcc ataccggcca cgatgatgtc gacggtacgc ttgacggccc gctgttcgac
     1801 gctgagacgc aggctcttgg gccggaagac cgggatgtcg tcgattttct gcagcatggc
     1861 accgttgcag aagatttcgt aagagccggg aatgatgtag ggctggattt ctttatccgt
     1921 acaatagttg acgatatccg ccttagtcgt gatagggata tccgagcaga tgatgagtgt
     1981 gtcgatgtcg caggattcca gttcctgacg ccaatccttc tgcgggtcca tgatgacgtg
     2041 ctggagcttc agcttgagct gaggctgggc actcatgcgg taatggacat gacgacattc
     2101 gtcttcgttg cccataatca tgacgcgtcg gacgggatga aggctctgtt cccaacgctg
     2161 gacggcccgc ttccaaaaac cgagcaggac gaattgtacg gccgtactgg aaataatcaa
     2221 tacccgggaa taagcgaatt catgaatgac aaaactcaag gccatgacca ggatgaggac
     2281 caggaagaga ctgacagcca gactgatcag gacttcgctg aagcgcttgt gttcaatcgt
     2341 ataaagaccg ttgatactga acatcatgac cgtgaagata acggcaatcg gcagcatccc
     2401 cgagtataaa gcctgatgta cttcccacgc actaggcggg ataatcagat aggtcaggag
     2461 tatgtaagtt agggtgatga tagtcacgtc accgagtaaa ccgaaaagtt tacgtgacgt
     2521 aggatagaca cgggtcttca tgaccttttt tcattccttt cgttgagttt ggggccggtg
     2581 gccccgtcgt tcgtcgttaa gcctcccctc ataggggagg tggccaaaag gccggagagg
     2641 ttggtttgcc ggtcgcagac cgcaggccgc cggtcgtgaa accatgccac attgaccagt
     2701 tggatatata gctgactggt cgctgtaaga acctctcagt cagcttcgct gacagctcca
     2761 gcacggctct ccgttttggc aaccgtcctg cacttccctc cggtcagtcc ctggcgggcc
     2821 aaaaagggcc tatccgggga gccatactac taacgtcttt tacgtttttt attatgattt
     2881 tcgtcgtaat agtagtagta atagtagccg ctcttgtggt gctgcgggac tttattcagg
     2941 atgacgccca ggatgttggc gtggactttt tctaagagtt ctttggcttg tttgccttca
     3001 tctgggctga tgtcgccgct gcccaggatg atgatggtac catcgacccg gctgctcatg
     3061 agggcagcat cggtgacggc caggacgggc ggcatgtcga ggaagacgta gtcataagct
     3121 tcagcagctt cctgcaacaa agcttccatc tttttcgaac ccaggagttc ggccgggttc
     3181 ggcgggatgg ggccgctggt gacgatgtcc aggttgggga agacgtcggc ctggatgact
     3241 tctttcagcg gccagtcttc ggcaaagcag ttgctcaggc cccgattgag caggctgaaa
     3301 cggcgatgga tgacgggctt gcgcatgtcg cagtcgatga gcaggatttt tttatcgtcc
     3361 tggcccatgg cgatggccaa attagcgaca gttgtcgatt tcccttcgcc aggaatagcc
     3421 gacgtaaaga caatcgtctt caattgtttt ccggcaccgg cgaattggag attcgtccga
     3481 atagcccgat aggcttcgga taccggcgac cggctgtcat aatgggtgat aaaatctatt
     3541 tccgccataa tttcctccta agtttcgacc acagcccctg gctgctgcct gtttctacct
     3601 gtaaggtatt cgtatcggga atcatcccca ggacaggcag gcccaattcc ttttccacgt
     3661 cttcggccgt ccgtatcttg cgatacatga gggattggac gatgaccgtc cccatgccga
     3721 cgatgagacc taacagggtc atgatggcca tgaccaggac tttccgaggt ttaatcggtt
     3781 tttctggcag tgtagccgca tcgatgacct gaacttcatt cgggaccggt ccgtcggggc
     3841 cgttggcgt
//
LOCUS       DAWCYS010000155         3810 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1289_length_3810_cov_4.758189, whole genome shotgun sequence.
ACCESSION   DAWCYS010000155 DAWCYS010000000
VERSION     DAWCYS010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_3810_cov_4.758189"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          800 a          995 c          980 g         1035 t
ORIGIN      
        1 gttctggcct tctgtagcgc cagcgccttt ctggatagcc cgtttgatgt tatctttcgg
       61 gatattgttt tcctttgctt tctgaagggc cagcttcaga cgcatgttgc cgaccgggtc
      121 cggaccaccc atgcggacag caatcgtgat ttcacggccg attttggtcg tgatcttacc
      181 gcggattgcg tcgtttttcc cttttttacg tttaatattc gcccatttag aatgtccaga
      241 cattcaaatg cctcccttta tttccaccaa tcttcgccgg ccaggcgatc taagatgata
      301 gctgctgccg aacggacaga taagtgatta tattccccgg ccccgtaaat cggttccagg
      361 atgaaatcaa atttttccat cgtttctttc gtcatcccgt agcccgtgcc aaagagcagg
      421 aggatcggcg tatcgtcttc ttcccgcttc tgccgcaact ccttatagga aatggtgttg
      481 gcataggtcc gcgcgtccgt cgtgacgacg taagggcgct tcccttctgc ggcggtgacg
      541 gcttcgacag ccgcttcgat ggacgggcgg atgtctacga tatcgaaagc ctgggtgcgg
      601 tagccgttat agttgcggcc caccgggctt tcccagaaat ccatgatttt atgcaccata
      661 tcgagctgcc ccggaatatg gtggatgatg aaatatttct tgacgccata agtcttggcc
      721 gaccgcgaaa tgtcatgcag atcgtagttg gtaatagccg tggcaatgac gttgaagttc
      781 ttatcatata tgggataatg aacaaatccg acatacaaag gggatttcat catttattag
      841 cccctttcag tttagccagt gtcttttggt cagctggcga caaatcgcaa tgctgcaaaa
      901 ggtccggccg taattccaaa gtccgcgcca gtgacgcttc ccgacgccag gcatcgattt
      961 tggcgtggtc gccgttccgc aggacctccg gtacggccat gccgcgaaag acggcaggct
     1021 tggtgtactg cggatactcc aggagcgacg aatagaaaga atcgtcagcg gccccggcag
     1081 ctgcgccgag gactccggga atcctgcggg ctacggcctc ggtaacggtc atagccggca
     1141 gttcaccgcc agtgaggacg tagtcgccaa cggaaatggt ctcatcggcc aaatcctgtt
     1201 cgacgcggta atcgacgcct tcgtaatggc cgcataataa aataagctgg tcgtatgtcg
     1261 ccagctcgcg tgccttggcc tgcgtaaagg tctgccctgc gggccccatg aagatgatgc
     1321 gccgccgcgg cacctgacgc cgcacggctt cgacagcggc atagaggggc gctgccttca
     1381 tgagcatgcc gcagccgccg ccatagatgg tatcatcgac catgtgatga cggtcgtacg
     1441 tgaaattacg gggattggtc acatccagat ccaacaggcc ggctgcccgt gcccgcccga
     1501 tgatgctgtg ttggaagaac gtttcgatga gctccggaaa gagtgagata atgtcgattc
     1561 tcattcgtcc acccactccg gcggatcgac gacaatcttc ttggcacttt catcaatgga
     1621 cttgatgaca tcgggaatgg cggccaggag tgtttccttg ccgtcatcgg cagtgacgac
     1681 atagacgtcg ttgctgcccg tgcgcagtac gtctgtaagt tttcccagtt catggccctg
     1741 aaggtcgtat acggccgagc cgaggatgtc gtagatgtag tgctggcctt tctgcagggg
     1801 cactaaatct tcgcgcggca cttgcaggtc ttttttgacc aacagttccg ccgcattgcg
     1861 atgatctacg acgacaaatt tcattatgac gacccgctta tgaagacgtg cggattctac
     1921 ttcatatttc ttgccgtcaa tgtagccgta tttcattttc aggaatcgtt tcggaaaatc
     1981 cgtatcggga taaatacgaa catcaccccg cacgccgtgc ggggcaacga tttgaccgat
     2041 tgtgaaaaga tctaattcag ccatcgtatt ctattggcgg atagaaacga tcttgccgtc
     2101 ttcaagcagg atttccgttc ccatcaattt ttctaaatca tcgccgacat tgacaacgac
     2161 agtctgttca agcgtgcctt gtctgatttc ggaaccgatt gtcaactgtt cagcttcttc
     2221 tctgtcgtgc tgtgccttag ccttagctgc ggcgagacgc tgtttctgtt cttctacttt
     2281 agcacgcaag gaaatcaacc cttgtgcatc gattttagct tgttcggtca tcatgcgttt
     2341 ggcctggaat tccaggttct gcatttcccg atcgatgtca ctcagggcct ggtcagcacc
     2401 tttgaggata cgatccttga gggcctgcgt aacctttgct ttgacagcga caggaacgcg
     2461 caatgtaatc tgatccatgg ttcctcctat acgatatcca ctgatacttt cttgttagcc
     2521 ttcactgccg cagccttgac aacaaggcgc aatgctttcg caattttacc ctgtttgcca
     2581 atgactttac ccatatcttc gggtgcgaca tgaagctgat agacttcgag gccttttttt
     2641 tgaatcttgg taactgtaac cgcatccggg ttcttgacga gggaccgggc gatacatgca
     2701 atcaattctt ccatagtatc cagcctcact tactttttgg atgcttcaaa ctgagccatg
     2761 atgccagctt ttttgaagat agaacggatc gtatctgtcg gctgtgcgcc tttgccgagc
     2821 catgctacag ctgcttcagc atcgacggtc agacgattgt ccatgtcttt ggacggatcg
     2881 tacatgccca acgtagcaac cgggcgaccg ttgttacgag catcgcggga atcagcgacg
     2941 acgacgcggt agatcggttt ctttttagca cctaaacgag ccaaacgaat tttaatcatt
     3001 gaaaatcacc tccttgatat agtatcttat ttccacaaaa aagggaaatc catttaaaaa
     3061 ggcagacgcg gcatgaaacc ccgtcgttta ttcttctgtg ctttcttggc ctgcttcatc
     3121 atcttctgca tttctgcgaa ctgcttgatg agacggttga catcctggac gcgcgtgccg
     3181 ctgcccttgg cgatacggcg cttgcggctg tccttgatga gcattttcgg attggcactc
     3241 gaccgttcct gggccgtcat ggatgtgatg atggcttcga ttcgtttgaa ttctttgccg
     3301 tccatgtcga ctttgtcgag ttctttctta tatttcccga tgcccgggat gaggctcagg
     3361 agactgccca tattgcccat tttcttgatc atatggagct gggacaggaa gtcatcgaag
     3421 gtgaaggtac cacttttcat ggtcttcttc atgttttcca tcgattcttc atcgaaagcc
     3481 gcctgggctt tttcgatgat cgtttcgacg tcgcctaagt cgaggatacg cgatgcgtag
     3541 cggttcggat ggaattcttc caagccgtcg agtttttcgc tgacgccgat gaacttaatc
     3601 ggtttgcccg tgacggcctt gatggacaag gcggcaccgc cgcgggcatc gccatcgagc
     3661 ttggtcatga tgataccgtc gatgccgagg gctttatcga aggccgatgc agccgtaacg
     3721 gcatcctgac cggtcatgga gtcgacgacg agcaggattt catgggtatg aacttcattc
     3781 ttgaggttga tgagttcgtc catgagggtt
//
LOCUS       DAWCYS010000156         3810 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1288_length_3810_cov_6.276431, whole genome shotgun sequence.
ACCESSION   DAWCYS010000156 DAWCYS010000000
VERSION     DAWCYS010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_3810_cov_6.276431"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          952 a         1046 c          991 g          821 t
ORIGIN      
        1 tgtgcaggca ttgcactttg cctttcactg gatggcgggg atcgcgggag ctgcttcggc
       61 ttcggcgttc tcgtccagtt cgccgtcgtc gcctagatgg gctggcgctg gcataacatc
      121 gccgtcgatg gcatccccta tcagtggcgc tgcgtcccgc ggctggaagt atccgccttt
      181 gggacggact acgtccgcgt cgtttttccc gaagacacga cgcagtggaa ggacgatacg
      241 ccgcctgaaa agggacagca gatttacgtc tatatttcac gggatacgtc gggcctcatg
      301 gaaatccagg gggcttcggc gtcgaagcct ttcgtcggcg gcgactatat gcaggccact
      361 gtcgtgtcct atcaggatgg ctttgtccag ttccaggttg gctttgaccg ctatcgcatg
      421 gcaccggagc tgaccgatgg catctacaac ctccagcccg atgacagcgt catcgccagc
      481 atccgcatga agcggggcga aggcgtcatc gaaggcatct tcgtcaatgg aatcccgttg
      541 gaaaatatca gcaatggcgc cgccatggcc aaggcccgcc aggaaaaaga ggcccagacc
      601 ttatttgacc ggcctcacct cgtcgatacc ggcatggtac cgccgaaaga agaatgatgg
      661 cacagaaaga aagcgagaat tttatgaaag cgtacgcgag aattgccaag attgctatga
      721 cagtcatgct gttggcagcg ttcttcctct tatcggggtt ccgggcgcag accatcatcc
      781 acgacgatgg cagcgaaaca caggatgtcc tgaaagtctc cgattcggct aaggggcaga
      841 aagatttgaa agccgatgcc gatgaattcc agaagcgcaa ttataccatt atggattata
      901 acaatgataa tggcgaaggc ttccgggcta tgaaaacgat taccaaggat ggagccagca
      961 agagctctgt cgaccacatt gtccacaaga cccacgatgg cctcatctgt tcgacttatt
     1021 acattgatta taattttgat gaaaatagta tccagaccct gcgcttaggc gcgccgatgc
     1081 cggaaaatgg cgtcgacctg gaatacatcg tctccttccc gtcggggacc cgcgtcgtca
     1141 gcaacagcga taaagccgat gaccagggca gtacctattt gtggaacctg tccaattcca
     1201 atccgacgga aatcaagctc caggccacag tctggcataa attgttcatc tatatcgccc
     1261 tgttcctggt cgtcctggtc ctgatcatcg tcctcatcat ggaacaccgc cgcaagaacg
     1321 tcatcagctg gaaacgggca gcgcatatgc gcaaactgga aatgatgctc ctctgtatcc
     1381 ctatcatcat cctgggctac atgggctatg aatattacgt cgggacccat gtgacggcag
     1441 cgtcgctgga taaagtggcc gaacagcagc aggaagaact gctggaaaac cgggaagaag
     1501 acaagcgcct gcaagatgcc gatgcccgta agcagcgcag cggcgaaatg gcagcggccc
     1561 gcatccgcag caaggccaat gacatttcca atgaactacg caatttgaag gtccagtacc
     1621 gcaacggctc catcagccgc agctcggctc gttcccaggc ccagagtctg gcccagcagg
     1681 ccaaggacat gctcagtgaa agcaccaatc tgtcccaggc cgaccgcgat gtcctggaac
     1741 agctcatcga caatgtcgta gccgaagcgg attctatcgg cgcatcggca ccgagccgca
     1801 gctcctctca ggaaacgcgg cgcagtgaat cggcttccga gtcccagagc acgacccgca
     1861 gccggtctga cgataagtcg acgaatgcga ccaagagttc cgattcgtcg aagagccgcc
     1921 agtcgtccga taacaatcgc gatacgggta aaacaaataa acaataaaga tttttaatta
     1981 actttttttc ttgttagtat ttcctgtttg tccaaaagat ggtatgatat gggtatgaga
     2041 attgacatta cagcatgaat gctgcttgta ctttttaagg aggtcatatc agtatgcatt
     2101 gtgtagaaga aattaaaaat ggtatttatt ggatgggctg caacgatttc cgtacggaat
     2161 tgtttgaacg gattttcccg attcccgaag gcgttgcgta caattcctat ttcatcgatg
     2221 acgaaaagac ttgtgtcgtc gatgccatgg ataaatcgtg ccgtgacgaa ttcctggaag
     2281 acgtcgaata cctgctcaaa ggccgtaaac tggactactt cgtcctccag catatggaac
     2341 cggaccatgc cagctcggct ttggctctcc tggaaaaata tccggacgcc aagattatcg
     2401 gccagccgca gaccttcaag ctctttgaac agttcttccg tcatcccatg acggaccgct
     2461 atgtcgccgt caaggaaggg gaccagctgg ctctgggcaa acataccctg cagttcgtca
     2521 aagcgccgat ggtccattgg ccggaagtca tcatgtccta tgacttgacg gatcacgtcc
     2581 tcttcagtgc cgatgccttt ggccagttcg gtacggtcgg caacgtctat gcagaccagg
     2641 tcgactttga agaaaactac ctcgattcgg cccgccgtta ctatgtcaac atcgtcggca
     2701 aatacggccc gcaggtcgtc aacgtcctca agaaggcatc gggccttcct atcgacttca
     2761 tctgcccgct ccacggcttc gtattccgca cgccggctac gattaagtat atcatcgaca
     2821 aatacctcca ctgggctaaa tacgtaccgg aaaagaaagg cgtcgtcatc gctttctcgt
     2881 ccatctatgg caacaccgaa cgggctgcct gcgtcctggc ccataaattg agccagcgcg
     2941 gcatccagga tatccgcctc tacgacgtcg ccaagatcca ttcgtcgtac atcacggcta
     3001 aagcctggga atacagccac ctcgtcctgg cagctccgac gtacaacctg aacctcttcc
     3061 tgcccatgga aaacttcctc cacgacttga agaccctcat gttccagaaa cgcacgatcg
     3121 gcgtcatcgc ctgccacagc tgggcatcgg ctgcctataa gaccatggtc gactacgtcg
     3181 aaaatcagtt caaatgctgc gaactcctgg aaccgacgat ggatatgaaa tcgtctctcc
     3241 gcgacgacca ggaatgtgaa ctcgacgaac tggccgataa actggctgaa agcattaaag
     3301 actatcccga tccgaaaact ttgatctaag gtggtgtaag cgtgatcatc cgtgacgaca
     3361 gcgccaagag cggcgataag cacgaacagg acaaggcccg cagcaatggg gaagaactcc
     3421 acatcgacct gggccgcgac ctgaccgaag acgaacagaa aatctttgaa aaattacgcg
     3481 aaggattaca tatgtaagga aaaggacttg ccctatgggc aagtcctttt tccgctgtag
     3541 gggcgcccgc cgttcgcagt ccgcagaccg caaatgtagg ggcgcccatg tggggcgccc
     3601 gcttttgcct agtggcgaga aaattttcat attccggacg ggctcgccac gtgcgagccc
     3661 ctacgaaaga cagatgtgcc ctgcgacctg cgactggcga ctggccaatc aaaacatacg
     3721 tttgcaatag ggccggcctt atgctcttct acaggtaaac tggggagctg tatcatggat
     3781 gttgagagga ttctagagat agtcgcgtgt
//
LOCUS       DAWCYS010000157         3786 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1298_length_3786_cov_4.540070, whole genome shotgun sequence.
ACCESSION   DAWCYS010000157 DAWCYS010000000
VERSION     DAWCYS010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_3786_cov_4.540070"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          946 a          980 c         1066 g          794 t
ORIGIN      
        1 gagatcatga tattccaaag ctgcttctac gagatgtttc aaatacggcg gtttagcgta
       61 tttgcgggca ccggccgaag cctgaaggat gatcggcgag ttcaattcag cagcggcctg
      121 ggtaacgccc tggacgattt ccatgttgtt gatgttgaat gcaccgatag catagtggcc
      181 ttcataggct tttttaaaca tttctgtagt tccaattaat ggcatgttta cattcctcct
      241 acataatacg taaaaatggt taatatatat aattccagcc ggcccgtcgg acgaaggcgt
      301 ccatatcttc aggcagccgg gaattaacgg tgatgaagcg gtggctcatg gggtgggtga
      361 actggagaca gtaggcgtgc agggcctggc ggccgatgaa gcggcgcgaa ccgccgtaga
      421 ggtcgtcgcc ggccagggga tagcccaggt aactgctgtg gacccgtatc tgatgggtcc
      481 ggcccgtgtg caggacgact tggagcaggc tgtagtcctt gccggctgcg aggcaggtga
      541 aatcggacca ggccggcttg ccgtcgaggc ggacacagcg ggtgatgatg ctatcgggac
      601 atcttccgat gggggcgtgt acagttgtga cccgcgacgg gtatcgtccc tcacagatag
      661 ctaagtacaa gcgcttatag cccaagtcct gcttatcgaa agcgtattgg atctgcggtt
      721 ccttggcgac catacagaga ccggacgtat tcttgtccag gcggtgcatg gggtggtagg
      781 aacagtgctg ccccgtctgt tcgtaatgat aggcgacggc gttggccagt gtcccgtccc
      841 gtacggctga tgtgggatgc atcaggagac ctgcaggctt gtcgatgacc atgaggtagt
      901 cgtcttcata gacgatgcca atggggatgt cctgtggcgg gaaggggttc ttttccggaa
      961 gggtgacgca gatctcgtcg ccttcatgga cgaagtcccg cgtcgaggcg gcctggccgt
     1021 tgatggtaca gatgccgttg tgcttgatac ggcgccgcaa tgtcgacgaa acgccgaaat
     1081 gtttcaaagc gccgcctaac gatgtaggcc gtatgatcgt ccctactgta aaatgaatca
     1141 tttgcatttc ccctatattg gctttgtcta catctattat acaaaatgaa aataaataaa
     1201 tccatacatt tcgtgaaaac atgtgggcta tattttagcc cgcaagggcc tgggagtgcg
     1261 gctggccaga aaaaatcatc tcctggaaaa ggaaacttga cgctccgcgt cgaaatagta
     1321 tatcatagga atgttatgta tccatttttt agatttacct taaaagagtg ttccctaaag
     1381 aaggaatttc acaggtggat agagaataat atatacgtca gcgttattga aatgaaacag
     1441 gtgatatgag tgaaacggtt ttccagcgaa tttatcgaac aggtccggca ggccaatgac
     1501 atcgtcagcg tcatctccga ttatgtgacg ctgaaacggc ggggccgcaa cttctgggcc
     1561 tgctgccctt tccataatga aaagaccgcc tccttcagcg tcgctgccga caaaggattc
     1621 ttttattgct ttggctgcca tgctcacggc gatgtcttcc aattcattat gaagaaagaa
     1681 aacctttcct tttcggatgc cgtcgaacgc ctggctgaac gggcccatat cgccctgccc
     1741 gtcgtcgaac ggtcggccga agaaatcgcc cgcgataaac agcgggaccg cttgtatcag
     1801 atcaacgaaa tggcgggcaa ttttttccat aattgcctga cccagactca ttacgggcag
     1861 cccggactgg cctatttcca caagcgccac gtatcggatg ataccatcga ggcctttaag
     1921 ctgggctttg ctccggaaag ctgggataaa ctgacgacgg ctttcatgaa aaaaggcgtc
     1981 actggcaggg aattagtcct cctgggactg gctaaagaaa agaacggcca cttttacgac
     2041 gccttccgcg gacgggtcat gttccccatc cgcgacggcc gcagccgcat cgtcggtttc
     2101 ggtggccgcg tcctcgacga cagcaagccg aagtatctca attcgccgga aacgccgatt
     2161 ttcaataaac ggcggctcct cttcgccatg gacctggccc ataaagcgat ttatgagcag
     2221 gggaaagcca tcctggtcga aggttatatg gatgtcgttt ccgctcataa ccggggcatc
     2281 cataacgtcg tcgcgtccct ggggacctcc tttacgccgg aacaggcgcg gctcttgcag
     2341 cgccaggcca aggaactcgt cctgggctat gacatggacg gcgccggccg ccaggcgacg
     2401 cagcgggcta tggaaatcgt ccgcggcctg ggcatgcgca tccgcgtctt gtccctgccg
     2461 cagggcaagg accctgatga atacatcaat gccgccgggc cggacaagtt ccgccaggcc
     2521 gtcgacgaag cgccgaatgt gctcgattac atgctcatgc gggccttgca gcgcttcgac
     2581 gtgacgacgc tggaagggaa atcgtccatt acggccatgg tcctgcccgt tgtcgccgcc
     2641 gtcgacaacc gggtcctgct ggaagcgttc ctgaagaaac tggcaggcca gctgcagata
     2701 ggggaaaacg ccgtgcgcag cgaattcaac aaatatgtcg ctgcccatcc ggaagcaggc
     2761 cagcagcagg tcgtcatctc ttcgtccgta tcccaggaaa atgccgcagc ccgcgggagc
     2821 ggtgccatgg ccgtagccga agagaatatc ctgcgtttcc tcctggaaaa gccggctgcc
     2881 tgcgggcgga tccacgccaa agtatcggca gacctctttg ccgatgaacg gcggcgccac
     2941 atttaccagc tcattttgga tacgtatgca caccaaggga tgtacacacc acatgacatc
     3001 cagcaaaaat taactcctga ggaggccgaa gaagtagcac gaattatggt tctacaagat
     3061 gtcccactgg acgaaaatgt actgatggat tatgtcaagc gctttcgcat ggccgattta
     3121 caaaagcagt acctggcgca cagccgcctg gctgccactt atagccggac tggcgatgca
     3181 aggctggccg aagagcttgc agcatgtaag aaaattaacg atgaaatgaa acaatggtct
     3241 tgaggagcag aaaggagcct gtacaccatg gtgaataaaa cgacagagga tacaaaagta
     3301 acgaagaaaa cgacgaaaaa gacgacgcgc cgcaagccgt ccaagaagaa ggacgctgcc
     3361 gccaagaaac cgttgagtga aaaagaacgc caggccctgg ccctgcagca catcaagcag
     3421 ctcctgcaaa tggggcagaa gaagggcagc ctgacgtata cggaaatcat gaacctcatg
     3481 gaagaagacg aactgacgcc ggaccagatc gacaagatgt acgaagtctt tgccgacaag
     3541 ggcatcgaca tcatcggcga agacgactcc atggcggaac atgatgatat agatgatgaa
     3601 gatacggaaa aagtcccgga ccttcacaat gtcgacctca gcgtgccgga aggcatcaac
     3661 atcgatgacc cggtccgcat gtatttgaaa gaaatcggcc gcgtccccct cttgtcggcg
     3721 acggaagaaa ttaccctggc caaggccatc gaaaagggca atgctcccga tgcgacagaa
     3781 gaagac
//
LOCUS       DAWCYS010000158         3779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1301_length_3779_cov_5.338614, whole genome shotgun sequence.
ACCESSION   DAWCYS010000158 DAWCYS010000000
VERSION     DAWCYS010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1301_length_3779_cov_5.338614"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          691 a         1191 c         1210 g          687 t
ORIGIN      
        1 ctcttaaaat acgttcaatc tgccatcaca aaagcgccgg acaagcggat tttatcactt
       61 tcttttgaaa ataaaacgta cttcgtcaag cgccgcatgg gcaatggccg caatgccttt
      121 gccaaacaga atccgtcggc cgccttttgg tgcgaagcct acaagatcat gaccgtcaac
      181 gcccgccttc ccctggcgcc gaaggtcgtc ctcctggatg aagacttctt cgtcatggaa
      241 gctgccggca agaccttgca gggcgtagcc aaggaagcgg aatacgccgc cgtccgccac
      301 gatgcctttg aaaaagccgg ccagggactg gctcagctcc acgccgccgg cctgcaccac
      361 ggtcggccgg ccctgcggga cattgcctac gataaggaaa ccgggacgat taccttcctc
      421 gactgggaaa atgaaaagaa attcgtcgac gcgccggcgc ccgtcctgga cctgttcctc
      481 ttcctgcaca gctgcttccg cgaggaatgg ccggacaatg ccctcatcga cgcagccgtc
      541 gccggctatg gccgcgtcga aggcagcagc cagaccatgg ccgccctgaa agaattcatc
      601 acagaccatc ataccctctt tgcctgctgt catgccctgg tccccttcgg ctggatcgac
      661 gtcgtctccg tcgacaaagc ccaagcgtat atcaccgcct tatagggcag aaaaggccgc
      721 agcacagcgg atatagccgc cggggagttc ctcggcggct ttcttcatct gcagggccag
      781 gaggatgggc agcaggtcgg cgacggcgag gcccgtcttt tccaggagct cgctcaccgg
      841 cgccgcccgg tcgaagggga tggcgtcgat gacggcctgt tcggccggac tcaggctggg
      901 tccttcctca tggcaggccg tctccggccc ctgccagccg cggcggcgga attcccggac
      961 cacatcgtca gcacaggtca ggggaatggc gccgagccgg aggagatggt tcgtcccccg
     1021 gctggtctcg gaaaagacac tgcccggcat ggcaaagacg tcgcgccctt cgtcgagggc
     1081 caggtcagcc gtaatcaggg agccgctgcg cagggccgct tcgacgacga tcgtcgccgc
     1141 tgccatgccg gcgatgatgc ggttgcgggc cgggaaattc cgggatagcg gctcgacgcc
     1201 gaaggcatat tccgagatga ctgcgccgcc gctatcgacg acctgccgga acagggcctt
     1261 gttctccctg ggataggtcc ggtccaggcc gttggcggcg acgacgacag tccgcccctt
     1321 ccttttcagg gcgccccgat gggcccgcgt atcgatgccc ctggcaccgc cgctgacgat
     1381 ggtgaggccc tgggccgcca gctcagcggc cagggtctcg gcggcattca ggccataggg
     1441 gctggccttg cgggagccga cgatggcgat actgctgctg tcgcgctcca tcgtcccctg
     1501 gcaaaagagg accagcggcg gctgggcgat gaagggcagc cagctggggt aatcgtcgtc
     1561 ccacagggcg acgggccgaa cgccataaaa agacagcctg cgctggaagg tatcccaatc
     1621 aaaaacatct cgttcttgcc tgagctgaga aaaaatcttg gccggaatcc ccgtccggcg
     1681 ccgcaggtct tcatcccaga cggcacccca ggcctggctg ggcgagcggc agacgtcgag
     1741 gagggcctgc aggcggcggc tgccgcagcc cttgagggac tggagggccg ctgcgtacat
     1801 cgtttcctgt tccaccttca tccccccttc atggctggtg catcttattg cggtaactga
     1861 tggcttcggc cacgtgatcc ttggcgatga ccggttcttt ggccaggtcg gcaatggtct
     1921 gggacacttt gatgatgcgg tcgtaactgc gggcactgag gcgcagggcg tcgaaggcct
     1981 cgcgcaacaa ctgctgggcc gcctcggtca gctggcacgt ggcccgtatc tcccggtggc
     2041 ccatatgggc gttgcaggta cagccataag gagccaggcg ctcttgctgg cgatggcgcg
     2101 ccgacaggac ccgcgagcgg atggccgacg acggttcggc ccgccgccgg gccgtcagtt
     2161 cccagtaggc cggccgttcg acgcggacgt ggaggtcgat gcggtccagg agagggccgg
     2221 aaatcttatg ggtatagcgg cgtatctcgc tgtccgaaca ggtacagcct tcgtcgaagc
     2281 cgtagcggcc gcagggacag ggattcatgg ccgccaccag gacgaagcgc gacggatagg
     2341 acagggccgc ctggacgcgg gcaatgtgga cgacgccgtc ttccaagggc tggcgcagga
     2401 cttccagaac cgaccggggg aactccggca gttcgtcgag gaagaggacg ccgtgatggc
     2461 tgagggtcac ttcgcccggc ttggggatgg acccgccgcc gacgaggccg gccatggaaa
     2521 tggtgtgatg agggctgcgg aaaggccgct gctgcaggag ccgccccttg cccaaaaggc
     2581 cggcgacgct gtatatcttg gtcacttcca gggcctcggc ttcgctcatg ggcggcagga
     2641 tagtcgggat gcgcttggcc agcatggtct tgccgcttcc cggcgggccg atcatgagga
     2701 cgttatggcc gccggctgcg gcgatttcca ggacccgctt ggcttcggcc tggccctgga
     2761 cttcggcgaa gtcgacgtca tagtccagct ggtccggcgc caagggttcc ggccgggccg
     2821 cgtccagcgg cttttcaccg gtcaggcagg cggcgacgtc gcagagcgta tcgacaccgt
     2881 agaccgtgag gtccggacag agcagggctt cggcggcatt gtcgcggctg acgaagaccg
     2941 tcttcatccc ctgggcggcc ccttccagga ccatgggcag gataccgttc accggccgga
     3001 tgcgcccatc cagggccagt tcgcccatga agagacattt ttcgcaggcc tcttcgggaa
     3061 tctggccgct cgacaccagg atagccacgg ccaggctcaa atccaggccg gacgtgtcct
     3121 tcttcaggtc agccggcgcc aggttgaccg tcaggcggcg catgggaaat tcatagccgc
     3181 tgttcttgac ggcagcccgg acccgctctt tggcctcttt caccgacgtc gatgccaggc
     3241 cgaccaggtc gaacgacggc aggccgttgg aaatatccgt ttcgacggcg atgacgtgcc
     3301 cgttcaggcc cagtgtcgcc gccccataga tacgcgcgaa cacggcgtca ccgcctttcg
     3361 gccaggaagg catgagcaat gtggcggatg ccaggctgtc cgttatctct tggccagact
     3421 tcgatgacgt cgaaccggca tggccgctcc caaagatcga actgggccag ataggccgag
     3481 gccacgcggg tgatctgacg gcgcttgcgt ttctcgacgg cttcgcaggg caggccgtaa
     3541 gaacctgtcg tccgcgtctt gacttcgatg aagaccagcg tatggccgtc gtcggcgatg
     3601 atgtcgattt ctcccaggcg gttgcggtaa ttacagcagc gcacgtgata gcccttttcc
     3661 tggcgcacaa aggccagcgc cgcttgttct ccataatagc ctaatagatt cttctccatg
     3721 gccattcccc tttctcaatg tatccgatta cgtacatcgt agacgagaaa gattaaaat
//
LOCUS       DAWCYS010000159         3720 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1317_length_3720_cov_6.187449, whole genome shotgun sequence.
ACCESSION   DAWCYS010000159 DAWCYS010000000
VERSION     DAWCYS010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1317_length_3720_cov_6.187449"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          999 a         1098 c          985 g          638 t
ORIGIN      
        1 aagaccccta tttatgtaaa ctatgttatt gcacataaca cacgtgggcg acccgctgga
       61 tatcaggcga cgttctcctt gaaccggaaa atcattcata ttctgagacg ggcttgccac
      121 ggcaagcccc tacaatttct acaaactaca aactcctctt cccctcaaag gccgccagga
      181 cgacgttgcg catggctacg cagccgatga tggtcagggc ggctacgacg aagggcgtga
      241 cgaagaccag gggattgttc tggaggatcg gcatggcctt gaagatgtac gtaaagacgt
      301 gtatctggct gtcgaagagg atgtgctgga ccagggccca catgaggacc atgacgacga
      361 tggcggcaat gtggaccagc cgctttttcc cctgataacc atagcgctta tacgggaaga
      421 aatagaaggc gaaggtggca atgacgaaga tgctcatgcc gatgaagcag aagcccatga
      481 tggccatgat gccgacgacc aggctctgcg tcacggacga ggcgctgacg gctgggaaga
      541 ggaagatgcc attcacggca gacggcaggg gcaggctgta gacgatggga tcgttgtact
      601 gggagttcct gtatttcagc cggcgccctt tccagaaatc gacggcccgt gccgccagat
      661 actgtatggc gacggtcagg aacatccaca gggcgaataa tccgacccgg acgggataag
      721 aatgccagca gtccaggccg gtcaggccgt tgagcaggcc gacagcgacg agcaggacga
      781 cggcgccgcc atacagagtc gccccggatg acgcataatc gccgtccctg cccgcacgac
      841 ggcggcgata gcccagccac aggggaatgg caaaggataa gataaaaaag gccagaatcg
      901 acgctgaggc gatatatggc ctatctgaag caaaggcacc aaggggcagc ggcaggatgc
      961 cgcacgacgc ccagacgacg gccagcagcc gatcgtgcca tgtctgtttt tgtaagttgt
     1021 ccattaacct acttcctttc ggcccggcag gagctgggat atggcgtaag ccaggagcat
     1081 ggtcaggctg atttctgcgt acatgccgac gatgaatacg atatcacggc caatggtcgg
     1141 ccattcccag aaaggcgcag ccttccaata gacacccatg aaggccgtaa aggccccgat
     1201 agccgcaaag acgatgaacc acatgagggc catgacccag cggcggatac gcccgccctt
     1261 ggggccaaac gtcgcatcgt ggcagcccac gtaggctacg gggatgacca gcgataaaaa
     1321 gacataactt accatggcta cgacggcact ggagccgctg tggatcgtat aggcaccgat
     1381 gggaatggga atgataaaga aacacatgaa gacacattgc cgcaagatat cgcgccacca
     1441 actcattttc tgcaagacct cctaaaataa tgcaaatgaa atcactttag tataccatat
     1501 ccctaaaaga aaaaaaagtt tgcacaaggc ctggcttttg tgtacaatag aaattatagc
     1561 aagtgatttt agtccgcaca gaaaggaaca tacatgacaa agaattttga aatcctcggc
     1621 attacgccga agatttgtga actcctccat aagcagggcg tcgacgaacc gacggccatc
     1681 caggcccagg ccattccgcc gctgttcaag ggccgcgaca tcctggccca ggcccagacc
     1741 ggcacgggca agacctttgc cttcctcctg ccgtcgctgc agcagatcaa gacggatatc
     1801 cacgccgaac aggtcctggt catggcaccg acacgggaat tggcccgtca aattgccgac
     1861 gtcgccgata cgctggcacc ggaactgggc atcgacgtcc tcagcctcat cggcggcaag
     1921 accatcgaga atcagctgca gaaactgcac cgccatccgc acgtcatcat cggcacacca
     1981 ggccggcttc tcgaccactg ccgccgccat tccctcgacc tcagcggcgt ccgccgggtc
     2041 atcgtcgacg aagcggacca gatgctccag gccggcttcc tggaagacgt cgataccctc
     2101 atcggcctga cgccgaaacg gcggcagctc ctcttcttct cggccactgt cccggataag
     2161 atcaaaggcc tggccaagaa gcacatgcag agcccgctgg tcctgaatat tctcgaaggc
     2221 cagaccatcg ccctggaaaa catcgaacag cgcatctaca tgatgacaga agaacagaaa
     2281 ctgccgaagc tctgccagat gctgaccgac atgaacccct acctggccat cgtcttctgc
     2341 aacaccaagg aatggacttc cctcctggca ggcaaactta tcgccaaggg cttcaacatc
     2401 ggcgaactcc acggcgacat gtcccagagc cgccgtaacc aggtcctgcg cgactttgcc
     2461 aaagccaaga cgcagatcct ggtcgccacg gacatcgccg cccgcggcat cgatatcgaa
     2521 ggcattaccc acgtcttcaa cttcgatgtc ccccacgacg tcgactacta catccaccgc
     2581 atcggccgca cgggccgggc cggcagcgaa ggcttgtcca tcatgttcgc cacggcagcc
     2641 gatgaaaact gggtccgccg catcgaacac aacatccacg cgacgatcac caaatatacc
     2701 ctgaccggtc aggtgaaggt caaggccagc agccgtccgc cgaaaaagaa aaagacctac
     2761 aaggacaagc tgcctgccag cacctaccag acgaccaagg aaaaacgcca taaacagcgc
     2821 cataagggcg gagacaaccg ccggcgccgc taaggaggtc ttactcatga aaagttatct
     2881 cacgatagtc ctcatgctct tcacgctcat agccgcaggt tgtacgccgg cgacgccgac
     2941 caattcccag ccgcagaaac agcagaccca gacccagcag caacagcctg gcgaaagcaa
     3001 gcagagtcag gatacgaaga gccaggccga cacgaaagaa acgccggtcg ccaacttgcc
     3061 gaccatcggc gatacccagg ccgattggga aaatgaatgg ggccatccct actcccaggg
     3121 cgacacgatc cgcgtcttcc ataatggccg ctaccaggtc gtcttccaga acggccgggc
     3181 cgttacagtg actatcaaag tccaagacgg tgaaaacgtc aatatcagtg acctcctgcc
     3241 ggaagatgcc cagaaacaat cggcatcgtc aaaaaatatc ggcggttccc agatgggcgt
     3301 cgaaaaatgg cacagcaaga ccctggaaaa agccttatcc tcgacaaaag gcaacttcac
     3361 ggtcatgaaa caaccgggca gcatcatcat cgactgcact ccggatttaa aaaaataatc
     3421 aatgtagggg cgccacgtgg ggcgcccgct tctgaaaaat agtgtaaaaa aagctgtcgc
     3481 ctcaaggtgc ggcagctttt tttgtgctgt aaggatcgcc acgtggaccc cattaggaat
     3541 gtgaatgact ttcttagcaa tctggctggg aaatcctaca acttctgaaa cgggctcgcc
     3601 acgtgcgagc cacgaacaac gggccacaac aaaaaagctc acgcaaaatg cgtgagcttt
     3661 tttctatggt gacccggtag ggattcgaac cctagaccta ctgattcgta gtcagtcact
//
LOCUS       DAWCYS010000160         3714 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1320_length_3714_cov_5.589779, whole genome shotgun sequence.
ACCESSION   DAWCYS010000160 DAWCYS010000000
VERSION     DAWCYS010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_3714_cov_5.589779"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          870 a         1034 c          899 g          911 t
ORIGIN      
        1 atggaaacct tggctaaact ccgtccggcc ttcatcaaaa acggcacggt taccgctggt
       61 aacgcctccg gcatcaacga cggcgcagct gctgttgtcg taatgtccgc tgaaaaagct
      121 aaagaactcg gcatcaaacc gctggctcgc atcatcgact gggcttcggc tggtgttgaa
      181 ccggctatca tgggtaccgg cccggttccg gcagtccgca aagtcatgaa gaagaccggc
      241 atgaacgtcg accagatgga cctcatcgaa ttgaacgaag ctttcgcagc tcagtccgta
      301 tactgctgcc gcgaactcgg cctcaacatg gacaaagtca acatccacgg cggcgctatc
      361 gccctcggcc atccgatcgg ctgctccggc gctcgtatcg tcgtcacctt gctctacgca
      421 ttggctgaac cggaaatcaa cggtaaatat ggtctggctt ccctctgcat cggcggcggc
      481 cagggcacgg ctatcatcgt agaacgtctg taatttaaca aaattcccta ttccctagca
      541 ataagtaatg gagatgtata gagtacgtaa tttttggaac gttctctata cattttcttt
      601 aaaatggtgt atgatagcaa tatcagagtg tcatttataa tatgacgctt tcgcacggtg
      661 catcctctca tactgataca ccggcatcta tttcaatacg taaaaatttt caaggagtga
      721 ttccaatgaa agaagtagtt atcgtaagtg cttgccgtac ggcaattggt aaattcggcg
      781 gcggtttcaa aaacacaccg gctgtagaac tcggtgctgt cgtcattaaa gaagcgctca
      841 aacgcgctgg tatcgaaggc aaagatgtcg acgaagtcgt aatgggcaac gtcctccagg
      901 ctgctcaggg tcagaacccg gctcgtcagg ctatgatcaa agctggtatg ccgattgaag
      961 tcccggctct tacggtcaat aaagtttgcg gttccggtct ccgctgcgta tccttggcag
     1021 ctcagatgat caaagccggc gacgctgacg tcatcgtagc aggcggcatg gaaaacatgt
     1081 ccatggctcc cttcgccgtt ccgaaagctc gttatggcta tcgcatgggc aatggtgtcc
     1141 tcgtcgatac gatgatcaaa gatggcctct gggacgcatt caacgactat catatgggca
     1201 tcacggctga aaacgttgct gaacgtttcg gcatcactcg tgaagaccag gacgcactcg
     1261 gtgtccgttc tcaggtcaaa gcttgcgaag ctatcaagag cggcgcattc aaatcccaga
     1321 tcgttccggt aacggttcat cagaagaaac aggacgtcgt catcgacacg gacgaattcc
     1381 cgcgtgaagg cacgaccctg gaaggccttg ctaaattgaa accggccttc aaaaaaggcg
     1441 gcaccgttac ggctggtaac gcttccggca tcaacgacgg cgcagctgcc ttcgtagtta
     1501 tgtccgctga taaagctaaa gaactcggcc tcaaaccgat ggctaagatt gtaagctatg
     1561 cttctgctgg cgtagatccc gctatcatgg gtaccggccc tgttccttct tcccgtaaag
     1621 cattggctaa agcaggcctg gaagtcaaag acctcgacct cgtcgaagct aacgaagcat
     1681 tcgcagctca ggctgtatac tgctgccgtg aactcggttt cgatatggac aaagtcaaca
     1741 tccacggcgg cgctatcgcc ctcggccatc cgattggtgc atcgggcgct cgtatcctcg
     1801 taaccttgct ctatggcttg aaagaagtcg gcggcaaata cggtttggct acactgtgca
     1861 tcggtggcgg ccagggtacg gcttgcgtcg tagaaaacat cgactaattt catctctcat
     1921 gtgtaaaaag gcttgtcgct tcggcggcaa gcctttttta gcagtcagct gatattttta
     1981 aatttctgta ggggcaacca cgtagggcgc ccgcttcagg attatgcaat cttccattca
     2041 gattgctggg aaactcattc aaattcccaa cgggtcccca cgtacgattc ctacagtgct
     2101 acgagccacg aaacacgaac cactaaactc tattcccatt tggaatatag agcgtgaaaa
     2161 gtggcccaaa tacatataac gttttttcaa aaagtattta attttcccgg caaaaatgat
     2221 agaattaatt tgctaaaaat ttcaagccat gcttatacgc atcactaacg aaagaacgac
     2281 tgataatgaa aactacatgt cgtaaaatgt ctgtggtaca gttgaccttt gtcactgctg
     2341 ccaacatgct tggcgccggt atcatcatgc tgccgaccaa gctcgccgaa gtcggtacga
     2401 tctcgattat ttcctggatt attacctcac taggctcttt agccctggcc atggcttttg
     2461 cccgatgtgg catgttcacg acaaagccag gtggtatggg cggttactcc gaatacgctt
     2521 tcgggcgtgt aggacatttt atggctaatt acgcctactc tgtttccatc gtaattgcaa
     2581 acgtcgctat cgccattacg gcggtcggct acggcgccgg tttccttgag acaaccttta
     2641 cgccgttaca gacgtgttta tatacgatcg ctttattgtg gatcgcggca gccctcaact
     2701 tcagcggctc ccgttattcc ggcaagctca gtgccatcac catctggggt gccatcatcc
     2761 cggtcctggg catttccatc atcggctggc attggttcag cccggctacc tggctggctt
     2821 cgtggaaccc caataacctg ggactcagcg atgccgtcgg caattccatc gccctgacct
     2881 tgtggtcctt cttgggactc gaatcggcag cagtcaacat ggatgccgtt gaaaatccga
     2941 agaaatccgt tcctattgcc actttcgtca gcaccctcgg cgtcgccatc atctacgtcg
     3001 cctcgacgaa cgtcattgca ggcatcgtcc ccaatgcgga aatcctggca tcgagcgctc
     3061 ccttcggcct ggccttctcg tacatgctcg gcgatacggc cggcaaagcc gtcatgggcc
     3121 tgatggtgtt ctcctgtgcc ggttccctct tgtcctggca gttcaccctg gcccgcgtct
     3181 tcaagaccag cgcccagcgc ggcctgttcc ccaagatttt cgccaaagtc acaagcgctg
     3241 acgtcccggt caaaggcatg ttgtgcatcc tctgcatgca gagcctcctg gccctcatga
     3301 ccatcagccc gaccctggcc gcccagttcg aactcctggc caacctggcc gtcgtcacca
     3361 acgtcatccc ctacatgctc tgtgccgggg ccctgacgac gatcctcacc aaggaacata
     3421 tccccaatga agtctgtaac agaaatacgt cgatattcct ggcaggcgta tccattatct
     3481 attgcgtata tgcattgacc accaccgatg ccacgacctt cctcggcggc tgtttcgccg
     3541 tcttcctggg atggctggta tacgtcatcc gcttcaccat catgaagcag accatcgtgg
     3601 aaattaaata aaaaaaaggc ttgtcgccgt gcgacaagcc tttttactgt aggggtcgcc
     3661 acgttgtcaa gaataacatc gtttacattt tgtgcaagga catcctttac actt
//
LOCUS       DAWCYS010000161         3704 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1328_length_3704_cov_5.221705, whole genome shotgun sequence.
ACCESSION   DAWCYS010000161 DAWCYS010000000
VERSION     DAWCYS010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1328_length_3704_cov_5.221705"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          742 a         1053 c          964 g          945 t
ORIGIN      
        1 catttcgtcg gcacggagcc tatcggttgt atactgacga tgcttttcgc tttcatccgt
       61 tcggccagct tcttcttaag ccgcgaccgc ttccgttcta accgaaagca cggattttcc
      121 tgcgagcagt agaaccgtgt gtaaaaggcg tgatgtaatt taccgttcag ttcgatgtct
      181 ttttcaatga tagtgtgctt gatacccaaa gaggaaagca actcagacac ctgcttcgtg
      241 agtgattcgt cacgctgaca gaattcgcac tgcccggctt tcgagcaata gccgtcgctg
      301 tccatcaggc cctggaccag cgccatgcgc tggccgagag agccccgcag gtattcagcc
      361 gggatgatct tatggttcag cagccccgtg gcgcgcaatt ccttttgcag gccgtctaag
      421 cacgctctta cgcactgccc ccgctgttcc tggatacttt ttacgggata accgcaacga
      481 tcgaactgct gactcaaagc aggcaggtcc ttctcgtggc atgttatccg ggcgctatca
      541 gagtcaccat tccccagcca gtagccgagg atgtaagggt ggatgggcag tgctttatgt
      601 gataacttta agggccgctg cataggcact cggtacctat actcgatgcc cttaccatca
      661 catcgctcat gcttataatt acgactcagt tcggccgtcg tacgggttac atagacgcct
      721 ttaggctttt tcaccgtaac tgtccacaaa tggccagaac cggctatgat gcgttcccca
      781 tcctcaaagg tcacctcgta gcagtcatta tgctgataga cgggcgacac ggcaaggacc
      841 ttcgtgggac tgccgctttg cgaaagcgcg gtatcgcctg cctgtagttc acccatcgtc
      901 gtccaaccgg acggtgtagg aatcggcgta tcgagtgcta aatccttgcc attcttccgg
      961 gcttctacca ggagcacatc ttggtacttg cggacccagc gtttgagctc cgcgtcgtag
     1021 atgtgcggcg cgaagatggc ttcgatgatg gctttctgga aaagctctaa tttaaaaggc
     1081 ttccccgcaa agggggcctg gccgtgccgc agctcatgtt cgatgaattg gatgcgcttg
     1141 ttcgacgcgt cataatccat cttcacgtcc gggtcggcca ggtcgtgtga taaacggtcc
     1201 agttccaagt ggatgtaacg gccggcgatg atttcgccgg acaggatctt atcacgatat
     1261 tcagctatgt agctcattcg taatcatcca atcctaaatc atcgtcatcg accgtccggc
     1321 cgagcacggc cgccagcttg aaaatgatgt tcgcataact ggcccttact ttcgggagca
     1381 gttttgaaac aggaagttct ttctgacggg acgggttcac cgggtccact ttgaccaggc
     1441 cggaagcggc ggcaaccata tgcaggcggt tcaactccgt gcgaagccgc gccgcttcta
     1501 caatggcacc atctaaaagg gccagctggt tctcgtcgac gccgtcgaac aaacttttga
     1561 ttcgttcgta ttcctcttcg attgtcatat tctcgcctcc tcacacggta taaaagcgtt
     1621 tctcgttcca gtatttccga tttcaaaaat cttcgccggg aaagtcaaaa ttttggtgca
     1681 gatgaaaaaa gggtattcgc cacggttcgg ctttttctta aaatttttag gcacccgggg
     1741 ggctatgctt gataatcgtc gaaccatcgt tcgatgtatc cctcccaggc tttcgccagg
     1801 ttcccgcggc ccgcgcgggc gttggctttg cactgctctt tcgacaggtc gcagaagatg
     1861 agttcggccc cgagcttccg ggccagcccc tcacgctcca gcttgaaggg atagccgccg
     1921 acgatgtagg catcgcccca ccggccagcc cggtgctgta tccggtccag cagtaaggtg
     1981 cggacagcga acacgtcgct cttcagctgg tccggcttat cgtacaagct gcagccggac
     2041 accgcgtaga acagcaggtc catgtcgacg atgaggtcac cgcggtgcat catctgccgg
     2101 accagggtcg acttaccgct gcatggtgca ccgtacacca gatacacgtg atgcgtgccc
     2161 tggccatagc gcttatgcgc tttgttgtgg cagtcgaagc agatgagctc gacgttcgac
     2221 ggattcaagg cgctgcttgc gtccgtcact gtctccggtg tcagctcttg gatgtgatgc
     2281 gcgatgagct tcgacgtgtc tgccatgtag tcccgatggc agacaggaca tacggtgccg
     2341 cgctcttgta tcagtgcccg gcgcaaggac cgccaggcct cggacttata gaacgcagcc
     2401 gcccacggct cagccataga agtccttcat ctccagttcc ttctgcttca tggccagcac
     2461 cttatcatca tagactttct tgtggcggtc cttcgggttc agctggaagt aatcggacag
     2521 ccattgcaag gccttcatcc tgtcggccag cttgatagac acaccatcac tggtggactt
     2581 gatttcctgt accagctggc cgtctatgtt gtccgagtct gccgagtgta cctctccgtc
     2641 tttgaccacg acgaactggt tgatattggc aaaggcgata cgcatataca tctcgacgat
     2701 atcgccgccg tcggcgaaca ggctctgcaa gcggagctcc ttgaggtatc ggatgtactt
     2761 gcttacgata ggcttatgca ataactcgta agccgtgttg cacgcggtga ggcgcttgca
     2821 tcgatacgcc cgctggtagc tggtaacagc attgaagcta ttgacgtaat agatgcagaa
     2881 cagccgctgc ttatcagtca tcccgtcctg gaaatgccgg tgcatcgact cggcctgccg
     2941 ctgtgcttcc tcgtcggtga tgtcttcgtc atcgagtttc ggggcatccg gctctgtcca
     3001 ttcatagttc ctggcctttg catccgggtc ctgtttctcc cattgatccc taaatttcca
     3061 gcgcttgatg tcgtcagccg tgacgccacc gacctgctgc gcgatgtcct tgttgcgtaa
     3121 ccgtccgccg ctggcaagcc agatttcgta ggcccgcttg cgtttcgggt tccggtcttt
     3181 atgtcgtccc atgctatcaa ctccttccgc tgggaacaga aaagccaccg gcacgaggct
     3241 tgtgtcgatg gctttccctg tgtaatttac tgatttgaaa aataaggagg tgtttacccg
     3301 atgagctcag gcaatcagag atgaatgtgt tgacttcttg tcccttctat ttccagttta
     3361 cattatatca ccggtcgtca gttcgcgcca ctccgttcga gttcgtcttt gtccgtttta
     3421 gttcgcaatt tcaaaatagc ggcgtttttc gcgtagatca cctcgaaatg ggccatcgca
     3481 tcgcgcttca gctcaaacag cttggtcttc ccgacgccga tatcagccat gacgtcggcc
     3541 caaccgaagc accacacata gtagcccatg aggacgtcac gctggtcctg atcctctatc
     3601 tgttcgatga ggcttgcggc ttcatccatg cggtcgataa aggaatccca ttcttcgttg
     3661 tcccggcgga tgatatcgtc gagcctggcg atcttatcgg agat
//
LOCUS       DAWCYS010000162         3701 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1332_length_3701_cov_5.117389, whole genome shotgun sequence.
ACCESSION   DAWCYS010000162 DAWCYS010000000
VERSION     DAWCYS010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_3701_cov_5.117389"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          731 a          993 c         1143 g          834 t
ORIGIN      
        1 gatgccaaag tcgatggtga tgcaggcgtc caggatctgt ccgacgagct tgaccttggc
       61 cggaatgttg cagatgtcgt cccagatgcc gacggcgacg atagccgtgc agcccaggag
      121 catcccgacc agttcatgct gcaaaggcac gcagaacaag acggctgcca tgaagccgat
      181 atagattgcc agcccgccga gccgcgggat gacatgagta tgtacctttc ttgcattggg
      241 ccggtccacg gcgccgatct tgatagcgag tttcttgacg ctcggcgtca ggagataaga
      301 aacgactaat gcaattacaa agggtacaac atattggagc acaacacacg cctactttcc
      361 attaatctat cagttcgtca gactgcgcat gggcgccggg atgcggccgc cgcgggcaat
      421 gaattcatct gacttgtatt tattgacatt gatgatcggg caatggccga ggaggccgcc
      481 gaattcaaca ttgtcgccga cgtccttgcc cggtacgggg atgacgcgga cggccgtcgt
      541 cttgttgttg atcatgccga tagccgcttc gtcggcgatg atggccgaaa tggtcgaggc
      601 cggcgtattg ccggggatgg cgatcatgtc gaggccgacc gagcagacac aggtcatggc
      661 ttccagtttt tccaggctca ggctgccgcg gccgacggct tcgagcatgc ccttgtcttc
      721 actgacaggg ataaaggcgc cagacaggcc gccgacgcgg gacgaagcca tgaggccgcc
      781 cttcttgacg gcgtcgttga ggagcgccag ggctgccgtc gtgccgcagg caccacagct
      841 ttccaggccc atttcttcca aaatcgatgc gacgctgtcg ccgactgccg gtgtcggagc
      901 caaggacagg tcgatgatgc caaagggcat gttgaggcgc ttcgacgctt ccatggcgac
      961 gagctggccg acgcgggtaa tcttgaaggc cgtcttcttg atggtttcgg cgacgacgtc
     1021 aaagggctgg cctttgacct gttccagggc gactttgacg acgcccgggc cgctgacacc
     1081 gacgctgatg cacgaatccc cattggtgac gccgtggaag gcgccggcca tgaaggggtt
     1141 gtcttcgacg gcattggaga agacgacgag tttggcgcag cctaaggcat cctggtcctt
     1201 cgtccgttcg gccaggtcct tgaagacgcg gcccatttcg gcgacggcat ccatgttgat
     1261 gccggccttc gtcgagccga cggcgacgga actgcagacg aggtcggtca cagccaggct
     1321 ttcggcgatg gagtcgatga ggatgcggtc gcctttgctc atgccccgtt cgaccagggc
     1381 cgagaagccg ccgatgaagt tgatgccgat ttctttagct gccttgtcga gggtccgggc
     1441 gatgtcgacg taactcgtca ggtcgctggc cccggcgacg aggctgatgg gcgtcacgga
     1501 gacgcgctta ttgatgatgg gcacgccgta ttcgcgggag atggcttctc ccgtttcgac
     1561 taagtgttca ccgtatttcg tgattttatc gtaaatattg ttgcacaagg tcttgccgtc
     1621 gctggcgcag cagtcgagga ggctgatgcc catggtaatg gtgcggacgt caaggttgtt
     1681 cttttgaatc atctgattcg tttcaagtac gtcttggatt gtcagcatat catttctcct
     1741 tagcctacac gatgcattgc gtcaaaaata gcctggttct gcgcccggat ttcaacgccg
     1801 agggcatcgc ccttctgttt gagcaaatcc tgtaaggtat ctaaggtaat atcggaatta
     1861 tccatgtcca cgatcatggc catgttgaaa atgccatcta agatgacctg gttgatcgtc
     1921 aagatattga ctttattttc tgccatgatc tggctgacgg cggcgatgat accagtgcgg
     1981 tccacgccaa ctacggtgat tacaactttc acagctgtcc actccttttc gttattaaat
     2041 tccatgcttc cctattatag cacaggtagc gtactgaatt ccactgtctg catacgaaat
     2101 gatagaattg acgacaaact tgcaactctg agctatttat tcaatgcctt gctgatgagg
     2161 gccttgcctt cttccaggag cttgccggcc tggtcgggcg aaacgaagct ttcagcgtat
     2221 aatttgtaca ggtcttcggt gccggacggg cgggcggcga cccagccgtc gtcggtcgtg
     2281 atcttgacgc cgccgatggc gaaatcacca tacggggact tggagaggac ggccgtaatg
     2341 gggctgccgc acaaggtctt ttccgtgacg tcggcttcgg tgagggcgct gatcttgtct
     2401 ttttcttcca gcgtcgccgg tgcatcgctg cgcatggtgt acggccggcc cagtttttca
     2461 cagaggtcgt tgtaatattc gatgggcgat ttgcccgtga cggcgcggat ttcagcggcc
     2521 aggaggcaca tgatcatgcc gtccttgtcg gtcgtccaga cgctgcggtc cttggcgatg
     2581 aacgagccgc cggcactttc ttcgccggaa aaggcgatct tgccatctgt atagaggggg
     2641 ccgaaatatt tgaagccgac cgggacttca gggagcggga tgtcgaggag cttggctgcc
     2701 cgggggagca gatccgtcgt gacgacggtc ttcgcgatgc ccaggccttc gaaattgcgg
     2761 tgcgtcatga gataatgggc cgcgacggtc aaaaaggcgt tggccgaaat catgccttcc
     2821 gcgccggaga cgatgccgaa gcggtcgtag tccgggtcat tggccaggac gaggttatag
     2881 tctgcggcct ggccggtgac ggccgacatg acataggccg acgagcagtc catgcggacc
     2941 ttgccgtcgt ggtcgtaatt catgaaggtg aactgcgggt catacgtatc gttgacgaaa
     3001 tccaggtcca ggtggtacgt gtcggaaatc tggtgccagt agttcatgcc cgacccgccc
     3061 agggcgttga ccaggatgcg caatttagcg tcgcggatgg agtccatgtc gatgatggag
     3121 tccagttcgg ccacgtagag gcccttgtaa tcataaggga cgacaaatgc gctgtccttg
     3181 gcctggtcat gggccatcat ctggacgcct tcgttgtggc cggccaggag acggttggct
     3241 tccctttcga tagccttggt gatatccgaa ccggccggcc cgccggtaat ggggttatac
     3301 ttgatgccgc catgttccgg aggattgtgg gacggcgtaa tgatcaaacc gtcggccagg
     3361 cgttcaccac gatggctgtt gtagcgcagg atggcccgcg atacagacgg tgtcggtacg
     3421 aaatcgccaa cactatcgac gtaagccgtg acgccgttgg ctgccaggac ggacaagacc
     3481 gtttcataag ccagtttcga cagataatgg gtatcttcgc cgacgaacag cggcccttcg
     3541 gcgccgaact gcttgcggaa gttgcagaca gcctgggtaa tagccgccac gtggtcctgg
     3601 gtaaagctgc cgttgccggc ctggccgcga tggcctgatg tgccgacacg gacctgctga
     3661 tgaggatccg ccacatcggg atgcgtcgta tgatatgccg c
//
LOCUS       DAWCYS010000163         3688 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1335_length_3688_cov_4.793009, whole genome shotgun sequence.
ACCESSION   DAWCYS010000163 DAWCYS010000000
VERSION     DAWCYS010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_3688_cov_4.793009"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          998 a          930 c          892 g          868 t
ORIGIN      
        1 aaactcggtg cttgaggatt gaccgaaccg aacgctggta ctgatgcttc cggccagcag
       61 actgttgctg ttaagaacga cgacggcaca tacacgctga acggctccaa gattttcatc
      121 accaacggcg gcgctgctga tatctacatc atcttcgcta tgaccgacaa gagcaaaggc
      181 aaccacggca ttacagcctt catcctcgaa gacggcatgc ctggcttcac tttcggtaag
      241 aaagaagaca agatgggcat ccatacttcg cagaccatgg aactcgtatt ccaggacgtc
      301 aaagttccgg ctgaaaacat gctcggcgaa gaaggcaaag gcttcaagat cgctatgatg
      361 actttggacg gcggccgtat cggcgttgct gctcaggctc tcggcattgc agaagctgca
      421 ctggcagatg ctgttgaata ctccaaacag cgtgtacagt tcggcaaacc gctctgcaaa
      481 ttccagtcca tttccttcaa actggctgac atgaagatgc agatcgaagc tgcccgtaac
      541 ctcgtttaca aagctgcttg caagaaacag gaaggtaaac ccttcaccgt tgatgctgct
      601 atcgcaaaac gcgttgcttc cgatgttgct atgcgcgtaa cgacagaagc agtccagatc
      661 ttcggcggct atggctacag cgaagaatat ccggttgctc gtcacatgcg cgatgctaag
      721 atcactcaga tctacgaagg cacgaacgaa gttcagctca tggttacagg cggtgctctg
      781 ttaagataag tgaagtttat gatgcaggct tggcatcagc caagcctgtt acataaaaaa
      841 agattttagg aggcaaacgt aaatggaaat attggtatgt gtcaaacaag ttccggacac
      901 agcagaagtt aagattgacc ccgtaaaaca taccgtcatt cgtgctggtg ttcctaacat
      961 ttttaacccc ttcgaccaga atgctctgga agcagctctt cagatgaaag atgctgacaa
     1021 agacgtaaag attacgttac tctcgatggg tcctgatcag gcaaaagacg ttcttcgtga
     1081 aggcctcgca atgggcgctg acgatgcata tcttctatcc gaccgcaaac tcggtggttc
     1141 cgacacgtta gctacgggct atgctttggc acaggctatc aaaaaaatag ctgctgacaa
     1201 aggtatcgaa cagttcgata tcatcctttg cggcaaacag gctatcgacg gcgataccgc
     1261 tcaggttggc ccgcagatcg cttgcgaact cggtattcct cagattacgt atgcccgcga
     1321 catcaaagtc gaaggcaaca aagttactgt tcagcaggaa aacgaagaag gctacatcgt
     1381 aacggaagct aacttcccgg tattgattac cgctgttaaa gacttgaacg aaccgcgttt
     1441 cccgaccatt cgtggcacca tgaaagcaaa acgccgcgaa atcccgaatt tggacgctgc
     1501 tgctgttgca gctgatgacg ctcagatcgg tttgtctggc tctccgacta aagtccgcaa
     1561 gattttcaca ccgcctcaga gatccggtgg tctcgttctc aaagttgaag atgacaacga
     1621 acaggcaatc gtcgaccagg ttatggaaaa actggttgcc cagaaaatca tttaatctaa
     1681 ggaggaacag tgaaaatgga tttagcagaa tataaaggca tttatgtaat tgctgaacag
     1741 tttgaaggca aattacgtga tgtatctttc gaattgttgg gccaggctcg catcttggct
     1801 gacaccatcg gcgacgaagt cggtgcaatc ctcattggta aagacgtaaa accgttggct
     1861 caggaactca tcgctcacgg tgctcataag gtattcgtat atgatgatcc tcagctcgaa
     1921 cattacaata cgacggctta tgcaaaagtt atttgcgatt tcttccatga agaaaaaccg
     1981 aacgtattcc tcgttggcgc taccaacatc ggccgtgacc tcggcccgcg tgtcgctaac
     2041 tccttgaaga ctggcctcac cgctgactgc acacagctcg gcgttgacga cgacaagaag
     2101 accatcgtat ggacccgtcc ggctctcggc ggcaacatca tggctgaaat catctgcccg
     2161 gacaaccgtc cgcagatggg tactgtccgc ccgcacgtct tcaaaaaacc ggaagcagat
     2221 ccttctgcaa ctggcgaagt tatcgaaaag aaagctaacc tctccgatgc tgacttcatg
     2281 accaaattcg tcgaactcat caaattgggc ggcgaaggcg ttaaaatcga agacgctgac
     2341 gttatcgttg ctggcggccg tggcatgaac ggtgaagaac cgttcaagac cggtatcctc
     2401 aaagaatgcg cagacgtcct cggcggcgct gttggtgcat cccgtgcagc tgttgacgct
     2461 ggctggatcg atgctctcca tcaggttggc cagactggta aaacagttgg tccgaagatc
     2521 tacattgcat gcgctatttc cggtgctatc cagcacttgg caggcatgac tggttctgac
     2581 tgcatcatcg ccatcaacaa agacgaagat gctccgattt tcaaagtctg cgactatggt
     2641 atcgtaggcg atgtcttcaa agttctcccg ctcctcacgg aagctatcaa gaaacagaaa
     2701 ggcattgcat aagttatcta accgtgcata atcaaaaggt ctatcgctat gcgatagacc
     2761 ttttttgcta ctggcagctg gccgttagtg tctgaccact agttcgtagg ggttcgcacg
     2821 tggcgaaccc gtttcagcat atgtgtggtt tcctattcaa gttgctagaa acatcataca
     2881 aatacttggc gggtcgccca cgggcaaccc ctacaaactc aaaaagaggc tgtcgcttta
     2941 agacagcctc ttttctctct tgacaacccc catttcctgc cgtataattt acgcatacat
     3001 agagatatgt aagatgcttt gaaaaggaca acacttgata gctcctttat tacagagaga
     3061 ggcacgatgg tgagagtgct ttataaagtc tggcaagtta ccgccttgga gcaggattcc
     3121 cgaagtaagt agggggtccc ggccgccccg ttatcgcgct tgagtacatc gggttttccg
     3181 gtgaactagg gtggaaccac gtgagttatg ctctcgtccc ttgaggacga gagctttttt
     3241 tattaaggag gatgtttgta atggcagaat tagtattaaa agacggaagc aaaaaacagt
     3301 ttcccgatgg catctctctt gccgaagctg tcaaacagct cagcaacagt cttgcaaaga
     3361 aagtattagt cgcaaaggta aacggtgaag tcacggacct ccgcgaaacc atcgtcgacg
     3421 gctcgaccgt tgaattcctc accttcgccg accaggaagg gaaaaatacg ctccgccata
     3481 cagcatctca tgtcatggca caggctatcc agcacctctt cccgggcgtc aagttcgcca
     3541 ttggcccgtc cattgaaaac ggcttctact acgacctcga cacagaacac cgtttcacac
     3601 aggaagactt tgctgccatt gaagctgaaa ttgctaagat cgtcaaataa aaccttccca
     3661 ttaccaaaga aggcttgtcc cgtgccga
//
LOCUS       DAWCYS010000164         3655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1354_length_3655_cov_4.984167, whole genome shotgun sequence.
ACCESSION   DAWCYS010000164 DAWCYS010000000
VERSION     DAWCYS010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_3655_cov_4.984167"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          926 a          940 c         1031 g          758 t
ORIGIN      
        1 ctttacaggt tttttcccca gggcgatgac accggaagca aaagaaacgt actgccctgc
       61 cgtcgatgtt ggctattcgg gcgtagcgga ccgggccgat gagtccgtcg tatccgtggt
      121 gatctccgtg gtgacacggg acccggatat gatccacggc gccgatgagc tttttcacat
      181 gctcgagtat atccgctttt gcttattgga tgcgaatccg gcctgtgata aatacgacgt
      241 caagaatggt acgatggaga cgtcggtacc tgatgaccag ccgtatccgc aatggtgggg
      301 ccgtattgac tttgaagtac atatcccgca gccgtccggc attgacccgt ttttattagg
      361 aggaccagaa catggacgac acaactaaga cagcggcggc cgcgccggca acagtcagca
      421 ccggcgccgc gaaatatacc gggcctgtaa tctatatcgg cccgggcttc cgtgattccc
      481 ggctgaacca ttgcatgatc tttgcaaatg gcatcccgga cccggaagca aacgatgacg
      541 tactaaaaca tttgtttgtt acgccggccg agctgaatca ggctctggcc gatgtacaga
      601 caaaagggac cgcgctttat accttttacc aggccgcggt cagcagacga aaagggggta
      661 aataggtatg gcatttttcc acggtatcaa gacttccgag attgatacgg ctgtcgtagc
      721 caccgcgcag acgacggccg gccttcccgt tgtcattggt acagcaccgg tccatctggc
      781 cagtgacccg aaaatcaatg aacccgtcat ctgctacagt tggacggaag ccgtaaaata
      841 cttaggctat catgaaaact gggataagta ttccctctgt gaagctatgt atgccgaatt
      901 caagctgttt gcagtagcac cggtagtatt tatcaatgtg ttgaacccgg ccaaacataa
      961 aaagtccgtg gccagcaagg ccgtaacggt agccaataag acggcgacca ttgcggacga
     1021 tgttatcctg agttcgttga ccgtatccgc agcgtcgtcc ggcacggcgg ctaaagctgg
     1081 taccgactac acggcggcct acgatgacga cggcaacgtc ctgatcacgc tcctgaaaga
     1141 cggcgcgctg gccagtgcaa cgtcggtata cgtcgcgtat gacgctatcg atacgtcgct
     1201 gattaaggac gccgacatca tcggcggcgt cggcagtgac gacacggcga cgggcctcga
     1261 acttatcgat atgatttatc ctaagtttag cttagtgcca ggccttttgg cggcgccggg
     1321 ctggagtcag catccggaag tcgcggctgt catggaagcc aagagtaaga aatttaacag
     1381 cctctttagc tgtacggtcc tgctcgacat cgatacgatg caggtcaagt cctacagcgg
     1441 atgcaatatg tggaagaccg gcaacggcta cacccataat aatgagtacg tcggctggcc
     1501 tatgggccgc atcggcgacc attgctatta catgtctacg ctggccatgg gccgtatcgg
     1561 tcagaccgac gcagacaatg acgatgtacc ctatgagtcg ccgtcgaata agacgctgcc
     1621 gattacaggc ctctgcttga aagacggtac agaagtcaca ctggacctga cacgagctaa
     1681 cctgctgaac agccagggca ttgtcacggc gctgacatcc ccagcaggct gggtactgtg
     1741 gggcaactat acaggcgcct atccgagcac gactgatcca aaagacgtat tcctgtgcgt
     1801 ccgccgcatg ttcgactggg acgatactgt tttcgtcctg acctactgga accgtctcga
     1861 taagccgggc aagccgcgca acattaagac tatcctcgat tcggaacgaa tccgcctgaa
     1921 cggcctcatt tcccgcgaat acatcttagg cggcagcatt gatttcctgg aagaagaaaa
     1981 tccaacgacg gacctcgaag ctggtatctt ccgcttccac aaaaaacgca cgccgccggt
     2041 accgatgcag gaaattgaca gcatttctga atacgacaca gccgctttta cggcgttgtt
     2101 tgaatagtga ggtgattaga tgtctgatgt aaataaaatg ccggaagtcc ttaatgacta
     2161 tcgtgtatac gacgaaaacg gcagtacttt gtacggtatc gcgaaattcg agctgccgga
     2221 ctttaaatcc atcacgcaga ctatcaaggg cgtaggtgtc ggcggcgaaa tcgaggcgcc
     2281 ggtcctgggg cagtttgaat cgttagaaac aaaaatcacg cacaacatca accgcgattg
     2341 gatccttaag cttgtaggcg gtcaggctgt tgccctggag gcccgtggtg ctaaccagta
     2401 ctgggacagc ggcgcgaata agtacgtcat ggacacggtc cgcgtcgtta tccgcggccg
     2461 ctcgaaagcg atggccggcg gctcctggga accagcgagc acggtagatg cgacgaatac
     2521 catcgaaacg acgtacatca agtatgaagt caatggtaag acgctgctgg aagttgacaa
     2581 gtatgcctat aaatttgtcg caggcggcga ggacattatg cagtctattc gtgatgcatt
     2641 aggtgtgtaa gggaggtatg agctttgaaa aaagaagaac aggtccagac ggtccgggat
     2701 gatcagaagg ttgagctgac ccgggcattg ccggacggca caaaaacatt gatcctcgat
     2761 tttgacaatg tatccggcta tacccttttg cgctgcgaaa aagcagcaaa aaaagaggat
     2821 gccagcattg tcgtaccggc gctgtcgcag gtatatcagg cacatgttgc cgctattgcg
     2881 gcgaatgtca aatacgatga catcttagcc ctgacagcta aagactttac ggccgtcatg
     2941 attaagacgc agggtttttt gctcggtacg gcatcgcagg accagccgga gggctaaccg
     3001 ccgaaaaact gcggctcgat gtactgcgga tggctaagta ttcgcatacg tctattgact
     3061 ttttccttgg cctgccggtg gcggacctgc gcggctggat gacgaccgta tccgaggaaa
     3121 tcgacaggca gaacaaagaa atagaaaaag ccgggaaggg gggcggcagc catggctaac
     3181 cgcgtgctgg aaatggcaat cgcaatcaaa gggcaattag atagcagcgt cggcggggcc
     3241 atgacaaaag ccatcgccca gacaaagcag atgcaagcgc agattcgcgc ggcgaatcgt
     3301 gaaatgacga acctgcaaaa gcaggcggca aaacagcagg ccagcaaggg ctatgtcgag
     3361 tatgacaccg aactggcgat gctccaggcg caggtcaaga agaatcaagc cgcgaaagag
     3421 tacgaggcta ccatggaccg cgtcaatgcc aagcagaaag cctctgcaaa tctgtcctct
     3481 gcggtcagca gcctgaaagc cggggccgtt gcggcggctg ctgtcgccgc gcctttaggc
     3541 ctcgcagtcc gtgaggctgt acagtttgag tcggcaatgt ctgacgtgcg gaaagtcgta
     3601 gactttgata caccgcagca gtttaaagag atgggcgacg atatcctgcg catgt
//
LOCUS       DAWCYS010000165         3632 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1362_length_3632_cov_5.682975, whole genome shotgun sequence.
ACCESSION   DAWCYS010000165 DAWCYS010000000
VERSION     DAWCYS010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1362_length_3632_cov_5.682975"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          829 a         1065 c          916 g          822 t
ORIGIN      
        1 ttcagcctgc tggcagagcg ataaaatctg ataggcatac tgtttcaagt tctcgccggc
       61 agcggtcagg taaatatgac gacccaggcg ttcgaataac aatatgccgt aatggttttc
      121 catttcatgg atggcctggg tgactgacgg ctggctgatg tggagctggc gggccgcttc
      181 ggtcatgctc agccgctcgc agacagccag gaaaatgcgg aaatggcgca aggtcatgga
      241 cttctcctcc tcatttcata ggtaaatact tatgatttat attatatcct agtattttac
      301 ttattgaaag agacagatta tactggagtc atctcaaata tcagtatagg agtgacttca
      361 tcatgcattt atccactatg atcgactggc gccacgccct gctcggtgtc gtcctgaccc
      421 tggcctgttc cctggccggc caatccctgg cagccctgcc gggcttttcc ctcatcggcc
      481 atctcgtcct ggccctgcta ttgggcatgg tcatccaggt ctgccgtccc gtcaccctgg
      541 cagcccggga aagtacgggc ttcattgcca acaaattcct ccgggccggc atcatcttcc
      601 tggggttcaa gctgaatctc atcgtcctgg tcagcgccgg tctgcaaagt ctggaagcgg
      661 cagtctttgt cgtcgccatc atgattcccc tcaattacgc cgccgcccgt ttcctgcgcg
      721 tcgatcacac cctggccctg ctcacggcct gtggctgcag catctgcggc gccgccgccg
      781 tcatgggcgt gtccggcgcc ctcaaggcca aggccgacca gtccgtcctg gccgtcgcca
      841 tcgtagccat cctgggcacg gtctttacac tcatcgaagt cttcgcccag cccatcctgg
      901 gctttacgga cagccagttc ggcgtcatgg ccggcctgag tctccatgaa atcgcccacg
      961 ccgtcgccgc cggcggcagt gccggcccgg tcgggacaga cagcgccatc atcgccaaat
     1021 tatcccgcgt cctgctcctg gctccggttg ccatcatcct gggcatcgtc gaagcctggc
     1081 gccagcgccg tcagtcccag gatagggatc agaaattcaa agtccccatc ccctggttca
     1141 tgggcggctt catcgcagcc agtgccatcg gctcctatat ccccgccatc ggattggccg
     1201 tccccatgct ggtcaagata gcttacctca tcctgggcat ggccatggcc gccctgggcc
     1261 tgaacgtcaa cttctcagtc atcgccaaag aaggcgtccg ccccatggtc agcgccatca
     1321 cctgctcctt cgtcatcctg gccctgtcct ggttcatcgt gcgcacgtgg ttctaggaag
     1381 tgcagaaaaa ggtgctgtcg catgaagaca gcgccttttt ttgcagtata atccccttac
     1441 ttcaggtatg cttcgacctg gtccgctttg agggcccggc ctgtcgaaac gactttatca
     1501 tcgacgacca gtgccggcgt actcatgatg ccatagcggg cgatctgggc aaagtccgtg
     1561 acatattgga cttcggcgtc aatccccaaa tgctgtacgg cttctttcgc agcagccagg
     1621 agggattcac atttgctaca gccactgcct aatactttga ttaccatatg atcaactcct
     1681 attctttgtc ggcctgcgct gatggctggc aggctttgcc cggtacgcag ccagtgtgcg
     1741 tacactttaa agtatctata aaggtgcgga aagccgtcaa ggtctggcaa ttcagggagt
     1801 aataggtatt cttccattct ttccggctag tcaccagacc gcattcagtc aacaccttca
     1861 tatgatgaga taaggtaggc tgggggagtt gaaaatactc cagcagcttg caggcacaca
     1921 tttccccctg cgtcaacaat tggacaatct gcaggcggtt cgcatccccc aaggctttac
     1981 ataataagga tacatctacg gcattcattg aatcacctca caatagaaat ccctgaaggg
     2041 cgttgaaact gtagccgacc aagatgattc cgatgacgca agtcaggata aagacgccta
     2101 acaatttcgg cttgatggct tttcgcagca agataagcga aggcagactc agcgtcgtca
     2161 cggccatcat gaagctcaat acggcgccta actgcgcccc cttccccagc aaggcttcgg
     2221 caatggggat agagccaaag atatcggaat ccataggtgc ccctaccagc gtggacagga
     2281 tgccgcccag cggattcggg cgccccagaa cgtcttcgcc ccactgctcc ggaatccaat
     2341 tatggatcaa cgcaccgatt ccaacgccaa tcaggatata gggaaagacc ttgcggaacg
     2401 tactggtcat ctgttctttc gcatagtcca aacggtcgcg ccttgtcaat tgtggaatct
     2461 ccgcttcact gacagccgga gcctgacgga tgaaatcttc aatttccgcc tccatgtgca
     2521 ggcgttctat gacggaaccg ccgataacgg cgatgatcag gccaaaaacc acataggcca
     2581 cggcaatgcg atagccaaag atactgatga gcaataccag gctgcctaaa tcgaccatag
     2641 gtgacgaaat caaaaaagaa aaagtaacgc ccagggggag gccggcactg gtaaatccca
     2701 tgaacagcgg aatggacgaa caagagcaga acggcgtcac cgtccccagc aaagccccca
     2761 tgatattcgc ccagataccg cggaaccgtc ccatgatttt ccggctccgt tccggtggga
     2821 agtaactctg gatataagaa ataagaaaga tcaatacgca taacagtacg gtaatcttca
     2881 cgacatcata aaagaaaaac tgcaaactgc cgccgacccg gctctgtaca tcgacaccgg
     2941 catattgtaa aattgccgca acgacctcat tcagccactg catccctaaa acctggttct
     3001 ggataaaaag ccacatatcc tccgcctcca tcacatagat ttttgtctat gtcatagatt
     3061 atacaagtga tatagatgat tgtcaatatg ataggttcaa aaagaaggac tgccacatga
     3121 aggcgtctgc cctcgtgcga caatcccttt ccaaagagta tgtactatct gctgaaaact
     3181 acaaacactg tacgataaac catcattttc ccgtatacgt cttgcgggca gcgacgactt
     3241 ggcggatgtg gtcggctacg gtcgtacagc agccgccgac ggcgacggca cctgctttca
     3301 tgtaatccag ggcataatcg ccgaaacggc ggtccgtccc gacgccgtgc caggtcttgg
     3361 tctgcgggtc gtattcttcg ccactgttcg ggtagacgat gatcggaagg tccgatgccc
     3421 ttttcaattc gccgatgagg ctggcaatgt attccggctt cgtgcagttg acaccgatgg
     3481 cctgcaggtg cggatagtta cgggccagct ggcgggcaca atcggccagg agctggcctt
     3541 cgcagttgtg gcggccgtcg cagcaggaaa agctgatcca atagggggcg cccagttctt
     3601 cggctatctg cgcttcgact tccgcttcca tc
//
LOCUS       DAWCYS010000166         3629 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1363_length_3629_cov_4.230274, whole genome shotgun sequence.
ACCESSION   DAWCYS010000166 DAWCYS010000000
VERSION     DAWCYS010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1363_length_3629_cov_4.230274"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          862 a          938 c          981 g          848 t
ORIGIN      
        1 cgccattgat gcccctacct aagaggaagg agtcttctgg cgaaactatg ataaagcagg
       61 ccgtccgcct tggataggga cggaaacatc ttgttatgag gaaaaattcg ctccatttat
      121 agatatatga agaaaaagta cagagttttt tgtgtcaagg agaaaaaatt atgatagaat
      181 aagggggtac atatacagag tatactaatt tgaggaggtg cgccgataat gatgaagcgg
      241 aatatcgttc tcgtcggcat gatggggagc ggcaagagtc atattggccg caagctggct
      301 cagcgcctcg gctggcaatt tgtcgatacg gaccggcgta ttgaacgggc tgtgggcatg
      361 accattgccg agttctttgc gagctccggg gaaaaagtgt ttcgcgaacg agaaatcaat
      421 gtcctgcagc aagtcagccg ctatcatgaa gctgtcattt cctttggcgg caattttcct
      481 atgaatgtcg gtacttatcg aatcctgcat cgccatgggt ttatcgtcgc cttattgtct
      541 gaaccgttcc gcgtggaaga acgggtcagc cgccatatag gcaagcggcc gactgtcgac
      601 tatgaaaatc ttcatgattt cgtccacacg atgatgaaac agtgggaaga ggtctatccg
      661 tactgcgact gcgtcattta taagactcgc gggggagcat cacagctcgt cgaatccaat
      721 gtccataaac tcaacgaaag gcatgtgcaa ttcattgcca gggaaaaaaa ggagattgat
      781 gaaaaatgat caacaaaatc ggagtattga ccagcggcgg ggatgccccc ggtatgaatg
      841 cggctatccg cggcgtcacg cggtatgcca ttcagaaagg cctcacggtg gaaggcatca
      901 ttcgcggtta tgaagggctg ctgagtcatg aaacgcgtcc cttgggccgt cgtgatgtcg
      961 gcgctatcat ccatcgcggt ggtaccatgc ttcggacagc ccgctgcctg gaattcaaaa
     1021 aaaaagaaac gcagaaaaaa gccgttgctt atttgcgtga actggaaata gatgctctcg
     1081 tcgtcattgg tggcgacggt tccatgcgtg gtgcccaggc actgagccgg ttgggattcc
     1141 cgacgattac gattccgggc accattgata acgacatgat ggggacgcag tataccctgg
     1201 gcttcgacac gacggtcaac accgtcctgg aatcggtcaa taaaatccgc gatacggcct
     1261 tctcccacga ccgcgtcgcc gtcatcgaag tcatgggccg tgaagccggt ttcatcgccc
     1321 tcaacgccgg catggcttcc ggcgccgaag cggtcctggt cccggaacat cccgtcgatc
     1381 tggctgccct ctgccgtcac ctggaagaat cgcaccgcat gaagaaactg agcagcatcg
     1441 tcatcgtcgc agaaggcgct gccaaagggg cggacgtcgt cgattatatc aaggaacata
     1501 cgtatttgtc gccgaatctg accgtcctcg gctacgttca gcgcggcggg gccccgtcgg
     1561 cttacgatgc catcatggcc ggccgtttcg ctcagaaagc cgtcgacagc ctgctggctg
     1621 gcacgtataa ctgcgtcgtc ggcctcatcg acaacagcgt cgtagcgaca ccgtattcgg
     1681 aagccgaagc cctgcgctat cccatcgacg aaaacctgtt ccacctcgtc catctcctgg
     1741 ggagataggc gttcgtggct cgtagtttgt ggctcccttg ataggggagc tgtcagcata
     1801 gctgactgag aggtttggaa agaggttgtc gcatgaaggt tctcgccgtc atgcgacaac
     1861 tttttttagt gcaacgtctc cggcccttaa ggccacctcc agctcggcgc tcccttttgg
     1921 caactggcct gctctttcct gcggtcagca cctgacaggt cccaaagggc ctatgaaggg
     1981 agacagggaa aatgcgttga catcttataa atatgcacgt ataatagagt agcgtatata
     2041 ttatcatatg ttatagagtg tcatttagag gagtgttatc atgtcgtttg attttagtct
     2101 gatttggaac tcattgcccc tgttgttggc aggtgccggt gtcactattg aaatcacggc
     2161 aatcgctgtc ggcctgggtt tcattttcgg cctgattacc agtgtgtgcc gcttgtcggg
     2221 cgtcaagact ttacaggtca ttgccgtctg ctacgtcaat attatccgtg gtacgcctat
     2281 gctggtccag attttcctga tttattttgc cctgccgatg gtcattggcg aacgcatcaa
     2341 ccccttcgtc gctgccgtcg ctgcctgctc gatcaacagt ggtgcttacg tttcggaaat
     2401 cttccgcgcc gggatccagt ccgttgataa aggccagatg gaagccggcc gttccctggg
     2461 cttgtcgtgg atgcagacca tgcgctatgt catcatgccc caggctttta aacacgtcat
     2521 cccgccgctg ggcaacgaat ttatttccat gaccaaggaa acgtccctgg tttcggtcat
     2581 cgtctttgaa gaattgaccc gccgcggcca gctcatcatc gccaagacct atggttcttt
     2641 tgaaatctgg ctgactgtcg ctgctattta cctggtcatg actttgacca ttgcccgtct
     2701 cgtaagctat ttggaacgga ggttcgcaac ggatgataga aattaaaggt ttgaaaaaaa
     2761 gttttggcaa gcacgaagtc ctgaagggca tcgacttgac cattcaagat aaagaagtcg
     2821 tcgtcatcat cggcccgtcc ggttccggca agagtacgat cctgcgctgc atcaactatc
     2881 tggaacagcc gacggctggg gaaatcatcg tcgacggcat cagcatgagc gatgccaaga
     2941 gcatcaacaa tatccgcaag gaagtcggca tggttttcca gcgcttcaat ctcttcccgc
     3001 acatgacggt cttgcagaac atcatcttgt cgcccatgaa gacccgcggt gaaagcaagg
     3061 atgaagcgac gaagacggct atccagctct tggaaaaagt cggtctggcc gataagaaag
     3121 acgcctatcc cgaaactttg tccggcggtc agcagcagcg tgtggccatt gcccgcgccc
     3181 tggccatgaa accgaagttc atgctcttcg atgaaccgac atcggcattg gacccggaaa
     3241 tggtccgcga agtattggac gtcatcaaga atttggccaa agacggcatg tccatggcca
     3301 tcgtcaccca cgaaatgggc tttgcccggg aagtcgccga tcgcgtcctg ttcatcgacg
     3361 aagggaaaat cctggaacaa gggaagccgg atgacatctt cttccatccg aaagaagaac
     3421 ggacgaaact gttcttgtcg aagatattgt aggagaagtg cgccctaggg ggcgcacgcg
     3481 gttcgcaggt cgcaggtcgc aagctgcagt aggggctcgc acgtggcgag cccgcttccg
     3541 catatgcacg attttcctaa ccagtttact aggataatca tcaaaattcc aaacggaccg
     3601 cccacgtggc gacccacatc agacatcaa
//
LOCUS       DAWCYS010000167         3620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1366_length_3620_cov_4.869565, whole genome shotgun sequence.
ACCESSION   DAWCYS010000167 DAWCYS010000000
VERSION     DAWCYS010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_3620_cov_4.869565"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          692 a          981 c         1066 g          881 t
ORIGIN      
        1 caggcgggcc ttgatttcct tgaagatctg cgtgccaatg aactgctgtt tttccgtcaa
       61 tttcaagtta tcgaagaagg gaatcatgtc gctgacgggc aaatccgtca attcggcgat
      121 gttcaggccg ccgatcttga tggccagcgc ttcgtggcgc aggcgtgcac cgtggcacgt
      181 cgagcagggc ttgacagtca tgaactggcc gaactggtcg cgcatcatgt ccgacgaggc
      241 ttcgcggtag cggcggcgga ccatgggcag gatgccttca aaggccgtgg cgaactgctt
      301 cacttcgccg cgcaggttct cgtattcaaa gacgcacgta tcgtctgagc cgttcatgag
      361 tttttcctgc aattcttcca gcaagtcatc gtagcagtta tccaaggtaa agccgtaagg
      421 ccgcagcagg ccaccgagct ggcgcatgaa ccacgaatcg gggttgctgc tgagggccgc
      481 tacggcgccg tcggcaaagg atttcttgtg gtccggcagg accagatgcg cgtcgacttc
      541 catggtgctg ccaatgcctg agcaggccgg gcaggcgccg aaggggctgt tgaaggaaaa
      601 gaggtgggat tcgatttccg gcaggctgac atggcagtcc gggcaggcga agtgctggct
      661 gaaggtcagg acctctttcg tcgccatgcg gaaaatatgg acgatgccat cggccaattt
      721 agccgccgtt tccagggaat ccgtcaggcg cgtcaagata ctgttcttga tgaccaggcg
      781 gtcgatgatg acgtcgatag tgtgcttttt atttttatcc agggggatat cgtcggacaa
      841 gagataaact tcgccgtcga cgcggacgcg gacatagccg tctttgacca gccgggccag
      901 gagcttctga tgagtcccct ttcgcccgtc gacgacaggc gccatgacca tgaccttctc
      961 gccttcgccg agggccatga cggcatcggc catctgctcg atggtctgct gacggatggg
     1021 cttgccgcat ttcgggcagt aggcctcgcc gacacgggcg tagagcagac gcaggtaatc
     1081 gtagatttcc gtgaccgtcc ctactgtcga ccggggattg cggctcgtcg tcttctggtc
     1141 gatggaaatg gccggcgaca agccttcgat gtaatcgacg tcgggcttat ccatctggcc
     1201 caggaactgc cgggcatagg ccgacaacga ttcgacatag cggcgctggc cttcggcata
     1261 aatcgtatca aaagccaggg acgacttgcc cgagccggac agacccgtaa agacgacgag
     1321 cttgtcccgg gggatcttga cgtcgatatt ctttaaattg tgctgacgtg cacctttgat
     1381 gacaatatat ttatcttgca cttcgtgcct cccgttatca taacgttcat cgtttgtcgt
     1441 tcatcgttcg tcgtgatggg ctaaaattta aaacctgctt cgaggaacga cccacgaact
     1501 acgaactacg aactacgttt acttcttctt cgcatcaggc cgctttatct tgcggcggta
     1561 gcggcgcttg ggcatgtctt cgccctgcat gtctttaggc agggtatcat cgtggctcat
     1621 atcggccagc tgctggcgca attggcccag gcggtcgcgc agttcggcgg ctttttcaaa
     1681 ttccaggttc ttcgacgcct ctttcatctg caacgtcatg gtcttgatga ctttgttcag
     1741 ggcctcgcgg gtcatcttgc cgcctttcct tcccttctgg ctgccatagg ccgcttccgt
     1801 ttcggcgact ttcgtcagtt cgatgaggtc gacgacgtct tttttgaccg atttcggcgt
     1861 aatgttatgt tttttattat atgcatcctg gatttccctg cgccgctgcg tttcttccat
     1921 agccttggcg atggagtcgg taatcttgtc ggcatacatg atgacctgtc catggacgtt
     1981 tcgcgccgcc cggcccatgg tctggataag cgacgtttct gaacgcagga agccttcttt
     2041 atcggcatcg agaatggcta ccagcgagac ttccggcata tccaggcctt cgcgcaggag
     2101 gttgatgccg accaggacgt cgaagacgcc ggcccgcagg tcgcggatga tgtcggcccg
     2161 ttcgatggtg gcgatatccg aatggagata gcgcacgcgg acgcccattt ccttcaggta
     2221 atccgtcagg tcttcagcca tcttcttggt cagcgtcgtc accaggacgc gttcgtcctt
     2281 ttccgtgcgc ttgcggattt cacccaatag gtcatccatc tggccggcaa tgggacggac
     2341 ttccactttc ggatccaaca ggcccgtcgg cctgatgatc tgctgggcca gattggtctg
     2401 gacttccatt tcataagggc caggcgtagc gctgacgtag acaatctggt tgatgcgttc
     2461 gacgaattcc tcaaatttca acggcctgtt atccaaagcc gacggcaggc ggaagccgta
     2521 ttcgatgagg ttttccttgc gcgaccggtc gccggcgtac atagcccgca gctgcggcag
     2581 ggtgacatgc gattcgtcga cgaggatgag gaagtcatcg gggaaatagt cgaggagcgt
     2641 atacggcgcc tgaccgggct tgcggccggc caggtggcgc gaataatttt cgatgcctga
     2701 gcagtagccc atttcttcca tcatttccat gtcatagcgc gtgcgctgtt ccagccgctg
     2761 agcttccagc aaatggccgc cgtctttcag cactttcagc cgttcgtcca attcctggcg
     2821 gatcgtcccc agggcccgtt ccatcttgtc cctggtcgtg actgaatggc tggccggata
     2881 aatggcgccg tgcttgcgct cggcaatgac ttcgcgtgtc aggacgtcga cttccgtgag
     2941 cctgtcgatt tcatcgccga acatttcgat gcggatgatg gagttatcga aggccgccgg
     3001 aaagacttcg attgtatcgc cgtggacgcg aaaggtcccg cgttcgaggt tcatgtcgtt
     3061 gcggctgtac tggatatcga cgagcttgtg cagtatttct tcctgcggct tttcctgccc
     3121 cagtcgcagg gatacgacga gttcactgta gtcttccggg tcgcctaagc cgtagataca
     3181 ggaaacggaa gcgacgatga tgacatcgcg ccgttcaaag aggctcatcg tcgccgagtg
     3241 gcgcaattta tcgatttcat cgttgatgga cgcgtctttc tcgatgtacg tatctgtcga
     3301 ggcgatatag gcttcgggct gatagaagtc gtaataactg acgaaataac cgacttcgtt
     3361 atcgggaaag aatgacttga attcactgca cagctgggca gccagggtct tattgtgggc
     3421 aatgaccagg gtcggtttct ggacggcttc gatgagcttg gccatagtga aggtcttgcc
     3481 cgtcccggta gccccgacga ggacctgggc ccattggccg gaacggatgc cgtctgccag
     3541 agaggcgatg gcctcgggct ggtcgccaat aggttcatat ggggcgacga cgtggaaagt
     3601 gtgggccgaa tcgtaatctt
//
LOCUS       DAWCYS010000168         3598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1374_length_3598_cov_4.989557, whole genome shotgun sequence.
ACCESSION   DAWCYS010000168 DAWCYS010000000
VERSION     DAWCYS010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_3598_cov_4.989557"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          810 a         1001 c          859 g          928 t
ORIGIN      
        1 ctttcttttt ctgttttgta gatgcgttca gcgatttcgt cgactttgtg ggtcagccac
       61 atgtaggccg tcgtaccctc acggtaatcg gcttcggcat acatatcgat gcggccatct
      121 tcccgcagtt gttcgacctg gcggaaggcc ttgctgtttc ctttgggata aatggcaaag
      181 gtcgatccgc cgaatttttt gactaccgaa aaggccggca catcgctggg cccgtcggca
      241 atataaatca tgttggggaa agcgacgcgg cgcagggcat cggacatctt ggcattgacg
      301 tcgatttccg gatgctgcgg cacgccctta ttgatttcaa acaaggcccg cgtcttggtc
      361 gtattatcga tagtatagcc cacttcactg atgattttat gcttttctgc atcgagccct
      421 tcaatgaggt cgcagcccca gatgtcttca acatagggca tgacggccga ccctttgatg
      481 atagccgaca tgcccgtact gacaatgtaa tgctccacct tgatgttgta ctcgcggtac
      541 ttggggttct cttcgacgac gcgctgaaga ttcttgaaga tttccgggac gccgtgataa
      601 tacttctgct tctggccaaa ggtcttcagc ttttcattat taaggccctt gaaaatccca
      661 ttctgtgcgt ccttgatgaa ctgattcaga taaatcgtgt cggcattgac cttgacgcct
      721 tccttggcat aatacgccgg caaggcattg acatgctgcc agaattttgc cttatcaata
      781 tgatattcct caaaaatcgg gtcctgcatg ttcccgtcaa tcaaagtctt gtcgaaatcc
      841 caaatcacag caatgatgtt ctgcatgaag ctcaccccgc tttcaggcaa tcagaaggaa
      901 aaggtatctt tcaaccccag ccactgctgg tctttatcgc tcagattcag cggcgtcccg
      961 tcttcgaggt gatagccggc ggcgctgggc gaaccctgat attcgccgac gagttggggc
     1021 cactggatgc cgatttccgg gtcgttccag gccatgccgc cttcatcatt gggatgccag
     1081 aaatcattga ccttatagca gaattccgcc acgtcgctga ggaccaggaa cccatgggca
     1141 aagccttcgg gaatgaggaa ctgcttctta ttttcttccg tcaattcgac accgtaccat
     1201 ttgccatacg tcttggaatc acggcgcaaa tcgacagcca catcgaaaac actgccgcgg
     1261 acagcacgga cgagcttgca ctgtggaaaa tgtttctgga aatgaagccc ccgcaagacc
     1321 cctttcgtcg acatggactg attatcctga acaaaattaa tatggaaccc cgcttcctcc
     1381 atatccttct gattatacgt ctccataaaa taaccccgtg catcgccatg caccgttggc
     1441 gtaatgacac aaagcccgtc aataccgccc acattctttt caacctttat ctgacccatt
     1501 aatatttatc tcccttcatg catgtttcct ttattataac ataatttaag taaacgcagg
     1561 ccgcaggtcg ccgttcgcag gccgccctta tcgttcatcg taggtcgttc gtcaacgagc
     1621 cacgaaccac tagccacgag ccactaaatt ccccaaatac cgcttcaagg catctttcca
     1681 gtcaggcagc ggccgaaatc cgttggcgac gagtttgctc ttgtccaagc gggaattaaa
     1741 gggtcgtttg gctttcgaca ggccgtattc ttccgtcgtg acggggatga ctttcgtcgt
     1801 atagccggcc tggcggtaga tttcacaggt aaaatcatac caggaaatat agccgccttc
     1861 gttagtcgca tggtaatagc cgtatttatc gctttcaatc atatcgacta acagacgagc
     1921 caaatcaaaa gtatacgtcg gtgtcccgat ctggtcattg acgacgcgga ccgtatcgtg
     1981 ggtcttaccg acattgagca tagtcttgac gaaattctta ccgttctgtc cgaataccca
     2041 ggcaatgcgg acgataaaat atttttccag cgtctgcgaa acggccaatt ccccttcaag
     2101 cttggtctgg ccataaacgt tgaggggctg gtaatccttg cagtccggct gccacggctc
     2161 cgtcccctgc ccgttgaaca cataatccgt actgagatag agcatcttac agtccaattc
     2221 cttgcagaca tcggcaatat gctgcgtccc accggcattg accgcccgga cctgagccac
     2281 tttgtcgtca tcttcggcca tatcgacagc cgtccaagcc gcacaatgaa ataacgaatc
     2341 gggctagact tccaaaatcc cacgccctac agaaacggag tcggaaatat ccaaagagac
     2401 atacgctgcc gccgtcaccg ccgtcccatc agccatacca ctgtaagccg gagccaaatc
     2461 actgccgata ccgacatgcc cccgcaaagc cagctcattc atcacatcat gacccaactg
     2521 gccattcaca cctgttacaa atattttcat aggatgtgtt ctctcccttc atgcatgttc
     2581 ctttttatca tagcatattc aatttgcagg ccgcagttcg ccgttcgcag gccgctctta
     2641 tcgttcatcg tagttcgttc gtcgttcatc gaaaggctcc cctgtgaagg ggagctatca
     2701 gcgtagctga ctgaggggtt cttttcccat cgcctctata cgattttgta ggggcgccac
     2761 gcggggcgcc cgtccggaat agcgatgcat aacgtactgc atgccatatg aacagtttgg
     2821 aaatattcct aactgttcac agcgggccgc cctttgggac ggtccctaca gatgcggctt
     2881 gtagccgcat aattttttat cgcctgcatg cgatttcgta ggggccgtcc taataaggcg
     2941 gcccgctacg cgctgaaggt atcccgaagc gtcgttgtca ctttgagtct gtcgccgcgt
     3001 agcggcatct gagtccgaga cagcctgcgg ccggcgaact gcggcctgcg tcgaggctcc
     3061 tagcctcgac tcccatacat cttctgataa tactgctggt attccccgga aatgatgggc
     3121 tgccaccaat cttcatggtc caagtaccac tggatggttt tcttgatgcc gtcggcaaat
     3181 ttcgtttccg gcagccagcc caattcttta tggattttcg tcgggtcgat ggcgtagcgg
     3241 cggtcatggc ctttgcggtc cgtgacatga tggatgaggc tttccggttt gcccagttct
     3301 ttgcaaatca atttaacgat gtcgatattc ctcatttcat tatggccgcc gacattatag
     3361 acttcaccga cgcggccctt ctggaggatg aggtcgatgg cccggcaatg gtcttcgcca
     3421 tagagccagt cgcggacatt gaggccgtcc ccatagacag gcagcagctt gtcatgcagg
     3481 gcgttgatga tcatgagggg aatcagcttt tccggaaaat gataaggtcc aaagttgttg
     3541 gagcagcggc tgctggtcac cggcagtcca ttggtccgag gataagccag acccagga
//
LOCUS       DAWCYS010000169         3584 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1384_length_3584_cov_7.085860, whole genome shotgun sequence.
ACCESSION   DAWCYS010000169 DAWCYS010000000
VERSION     DAWCYS010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1384_length_3584_cov_7.085860"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          869 a          815 c          820 g         1080 t
ORIGIN      
        1 aagctgaacg aagcgaagat tatcccagtg gcattggcaa ttacctgggc aagaactggg
       61 gcttgttctt aggccttctc tacttcctga tgctcattat ctgggtcttc gtttattcaa
      121 cggccatcaa taatgacagt gcgtcttttt tagtcacctt tggtgtgaca gatcattcgc
      181 tggctaaaaa tccgttatac gggctggcca ttatttgtgc tcttgtcgcc attgcatctc
      241 gtggtgaaaa agtactcttc aatatttcgt cgctgatggt cttgacgaag ttgtgtggcg
      301 tagctgtatt gggtatcatc atggttcaga actggaattt agcgaatgta ggcagcttcc
      361 ccgattttgg ctatctcatt aagcagacca tcatcatgct gcccttcacg ctgacatcta
      421 ttttgttctt gcagagtttg agcccgatgg ttatttctta tcgttcccat aataagtcga
      481 ttgaagtcgc ccggtacaag gctttgcggg ccatgaatat cgcctttggc gtactctttg
      541 tcaccgtatt tttctatgct atttcgttca acttggctat gggccatgac caggctgtta
      601 tggcttatga aaacaatatt tctgctttag cattggctgc ccagggtatt cccggcactt
      661 ccattaagat catgagtttg attttaaata tttttgctgt cgttacggcc ttcttcggcg
      721 tcttcctggg cttccgcgaa gcttgccgcg gcattgccat gaactgtttg aaacgcatca
      781 tgccggaaga taagattaat gtacgtgtcg tttcgttggg catcttggtc tttgcggttc
      841 tcgtgtcgtg gggcgccatt attctcaatg ctcccgtttt gagctttact tccatctgta
      901 gtcctgtttt tggcatgatt ggctgcttga ttccggctta tctcgtctat cgggtgccgc
      961 ggctgcatga cctgaaaggt gtttcgctgt atctcatcat tgcaacgggc atcttgctga
     1021 ttatctcgcc attcctggct ttttcgtaag cttgtaacgg atttcataga ttgatttttt
     1081 aggagtgata ataatggaaa cctgtactat gtggaataaa atgattgaaa tcatgaagca
     1141 agatatcaaa ccggctacgg gatgtacgga accgatttcc ctggcttttg cagcggctac
     1201 ggcggcgaaa gaattgggag aacctgtaca atctgtcaaa gcctttgttt ccgctaattt
     1261 gatgaaaaat gggatgggcg tcatggttcc tggaaccggc atgccaggat tggctattgc
     1321 cgctgctgtc ggggctttgg gcggtgatgc tgatgcaggc ctccaggtcc tgaaatcctt
     1381 gacgcctgat gtggtagccc aagggaaaca aatggtagcc gatggcaagg tcaccgttgg
     1441 tgtcaatgaa acgacaaaac atatccttta ttcggaagcc aacgtctatg gggaatgcca
     1501 tcatgtccgc gccgtcatcg tcgatagcca tacgaacgtc gtcttaatcg aaaaagatgg
     1561 gcaggtcatt tttgaaaaac aagcgagctg tgataatggc gaagccgata aaattgcttt
     1621 tttgcagtcc ctctcgatca aagatatttt ggaatttgct gaaaatattc cccttgccaa
     1681 tatccagttc atcaagaatg cagaaaattt gaatgatgcc ttgtctaaag aaggtttgac
     1741 tgggaaatat ggcctgcgca ttggctgcac catggaaaaa cacattaaag aaggcttgct
     1801 ggcaggcgat ttggcccgtg aaatccaagt ccgcacggta gccgcttcgg atgcccgcat
     1861 gggtggcgct gcttgtccgg ccatgaccaa ttccggttct ggcaaccaag ggattaccgt
     1921 gtccgagccg gtaacggtgt cgctgaccat atcaaagcct ctggcgaaga acgcgtccgg
     1981 gccctggctt tggccagtat ggtagctatc tatgctcaca gctatttgcc caagttgtct
     2041 gccttctgcg cgaccgttac ggctgccatg ggggcagcgg ccagcatggc ctggctcctt
     2101 gaccgcaaga cgccctatca gactatttgc aaagccattt cgtccatgaa tggcggcatc
     2161 gtcggcatgg tctgcgatgg cgctgccgat agctgtgcca tgaaagtcgc ttcggcagca
     2221 gaaatcgcct atcgttcagt cctcatggcc ttagccggta tccgcgtaga aggtacggat
     2281 ggactcgtcg ctgatacggc tgatgaatgt atccgtaacg taggtctttt agcctccaat
     2341 gggatgcagc agaccgattg tgaagtcttg cacatcatgt tgaacaaaaa taaagaagtt
     2401 gccggggcat aatttccatg acaaaataaa aaatcccctg atgctatcca ttatttcgat
     2461 agcatcgggg gaattttttt agtcaatgtg atattccttg tataatgctt ttcggactgc
     2521 ttcgtcttct atggctaaat ccaaatcatg taagcggcca tcagccttca acaatttcag
     2581 caattttgcc agattatctt cgcccttgcg ctgcccttcg acaagacctt cctgacgctc
     2641 ttccatgctc catttctcta attctcggca cattttagcc acctccacag gattattttt
     2701 gtaacgattg acaacttctt tcaaaggggg ccaaaacatt tcttcagcag ttgtgctacg
     2761 gaaatcattc attagcttac ctatatcatc ttggccccga taagccccgt tgacgtaaat
     2821 gatgtgggac ccatcctcga aatgttgatt tgtttcttcg atgatgcggt taatatggta
     2881 tcgggatagc ccatgctcta atatatcatt ttccgtaatg aagattacgt aggtttccgg
     2941 tagctggtca tagtcgaacc ctttttttag attgttgaga tctaacacac tgccattgaa
     3001 acgggcccgt cgagctccag caccttgatc tgaacgctgt atctcaatat cgtattctat
     3061 acccatagca tcagtagcat ggacatccag cctgacgccg tgtccttgta aattaggaat
     3121 tgtatcctcc acatggagtg gttgcacttc taggtcaggc tgttccataa taatccgaag
     3181 aatacattca acagcaggaa tattatcttt caaacaccgc ttcatgaaat cgtcatccat
     3241 taaacgccat cgtttgataa gattcagtgc tcgctgatgg cgtcttgcct gttgcttatt
     3301 atctgattta tcgttcatca gcaatccctt tcatttccat tatacttagt tcagaaaatt
     3361 actacaatag attatttgaa agaaaataag tagataaacg cgcgttctac cgaatatatg
     3421 ttcattataa catggatata taatttgaac aaatgtttta tatgcataaa tgaaaagtcg
     3481 cgcgaaccaa tgttatttgg ttttgtacaa aaaaattccc tgatactgtg taagcggaaa
     3541 ataaatcagt gtatagaaaa agccccagac ttatgctatc cttt
//
LOCUS       DAWCYS010000170         3578 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1387_length_3578_cov_6.364178, whole genome shotgun sequence.
ACCESSION   DAWCYS010000170 DAWCYS010000000
VERSION     DAWCYS010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1387_length_3578_cov_6.364178"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          868 a          983 c          880 g          847 t
ORIGIN      
        1 caagggtcgt cccattgcgg ccgtcgatgc ggacaggcag cagggcacac gacaacatag
       61 gcacatcgtc gacccagacc gtacagaggc cacagttcgt cgtttcacag ccgcatttga
      121 cgctgtaaca gccctggctg cggagaaaat catagagcat caggcccggc gcgacgtcag
      181 ccgtcgcctt cttgccgttc agccaatacg taatggtcat cactggtcac ctcccagaac
      241 ttttgccaat aagcgccggc agaggacgac agccatatcc tggcggtaat cggcggaacc
      301 gcgcatattg ctttcataag tcatggaatg ggcaatttgt ttggcagccc cttccaagcc
      361 ctggcgacgc acatcagctg ccggcagacg gacgacttct gcccgggccg ggcgggcgcc
      421 gatggcacag cgcacgtttt ctgcgtcagc cgatacggcc acggcgatga cagggaagtc
      481 cgtttcattg aggcgcagcg aatcatacgc cgtctggcag gcctttttcg ggatgatgat
      541 gtgagtcacg atgtctttcc ccggcccggt ctgaccgaaa tcggccaggc tgacgatacc
      601 gcccttgtac aattcgacct gggcatccag ggccaggaac agcgtccaga tgtcggaaaa
      661 gccgaagcgc ccgctgatac tgccgccgac cgtcgccata ttgcggaact gggtcccgac
      721 gatatggcgg accgcttcct tcatggcgcc ttgagtgaaa gcagccagcc catcgtggcc
      781 ttcgagctgc cctaacgtaa ccatagcacc caggcggaaa gagtcctccc cttcttcgat
      841 ctgggccagc cccagcgccg acaaatcgat agcctgggca atgcgccgct gcggcgacaa
      901 gcggagccag cagcagccgc cgaggacgac agcggaccgt ttctggttaa gctcccacgc
      961 ttcttccaga gaggccgctt tcgtatattg cgcaatattc ataaaaaacc ctcctttaca
     1021 gggcaaaaaa taaattcaat gcatagtcca tcaagtttat tataccagca aaaaagggcg
     1081 ggccgcaaga gactgtatga gacataaaaa aaggatttgt cgcacgtgcg acaaatcctt
     1141 ttcttgtagg ggcgaccgcg tgaggcagcc tctctggaat atgtacgatt tcccaatcag
     1201 cttgctagga aagacatcca aatccctaac gggtcgccca cgggcgaccc ctacaaactc
     1261 cgtactataa ctataaagag aaaggccctt gcattatggc tgtattttga aaagtctttc
     1321 tgtatttgct ttggcatgag ctgtcaattc ttcgacagat acgcccatat acccggccag
     1381 ggtctgtgcc acataggtga tattgttggg cacattggtc cggggcaggt ggaagccccg
     1441 cggcgtcagg tacggtgcat ccgtttcgat gaggacccgg tccaaaggca ggatggatac
     1501 agcatcgcgt aagggagccg cccgccgttc atcgcagatc cagccggtaa taccgatata
     1561 aaagcctaaa tcgagcatct tttccagcgt cttcttgtcg cccgtataac agtggacgac
     1621 cgattgcgtg caaagccctt catgcccctt gaaacaagcc aggaagtcat cagctgcgtc
     1681 ccgttcatga aggaacatgg gcttacctaa ttcttccgcc atagcaatga gggctttaaa
     1741 aaagtggagc tggttctctt tcttagaata catgtggtcg taatcaagac ccgtttcacc
     1801 gacagcgacg actttcggat tcgtcgtgat gatgcgccgg atttcctcca aatccgcctc
     1861 cgtcgcttca tcagccacgt gaggatggat accggccgtg ccaaaagccg tgtgattgcg
     1921 gacaaactga tcgaccagac ggttctcccg ggcctccgac ccagtcagga tacagcagat
     1981 accgtccgcc accgcatcgg caataatttt ttccggtttg ggaaaagacc aggtaaataa
     2041 attgagccca atatcataat attttatatc catgtcagac acctcgtgaa aataagattc
     2101 cacgaatatt gtaccaaaaa aacaggttcc tgcgtagcta aaaaaaagct tgtcacacaa
     2161 cgacaagccc tttaaggtta ttagcggcta gcagttagtg gttagcggat gttttttaat
     2221 ttaaaggttt tctgcctttt tcccatcctt cttgcttgcc tttcatcagg ccatcttcgt
     2281 atattccttc tcctaagtta cacatgactg ctaaatcccc ctctccttct tgtgtcaaat
     2341 caattccaaa atcattatgc agggttgtaa tcttttcact ggctttcttt gacgataccc
     2401 attttgcctc attgtaccaa attttgattt ttttgtacaa tccacattca tggcaaaaaa
     2461 agagcaccca ggaaattttc ctaggtgctc tctgtccagt aatctggcgg agtgagagag
     2521 attcgaactc tcgcaggcct tacgacctct aacgatttag caaaccgtcc tcttcagcct
     2581 cttgagtatc actccatgcg tcaactgact atgtcagtat actacgatgg agcgatattg
     2641 tcaagttatt ttttcaatat atcttctacg gccttggttt tattgttcat catattcgta
     2701 tagcccggtc ggatatcggc tttcaagacg acttcagccc gcaggccctg gtacgtcagg
     2761 tattgcgttg cttttttgac gactgccatg acatcgtccc attcgccttc gatttcggtg
     2821 aacatggaat tggtccgatt cggtaagcct gattcccgga taatacgcac gatttcagcg
     2881 acatacggag cgaattcatc gccagtcccg cacggggcaa tagctacagc aattaaggta
     2941 ttcatgatat tatgcctcct ctacagtcaa tgggttctgg gcaatcgcat cgggcgttgc
     3001 cacatagagt tcgtcgatga aatccgtctg gaaagagcct gtccctttgg catgagctgc
     3061 ggcccgtgta ccggccaggt tatagacggc agagccggta atggcggcga tgaacggatc
     3121 tgctacggct gcatagacag ccatgacacc gccaagggaa cagcctgtcc cggtaatgca
     3181 gggcatcatt tccgaaccgc cctgggaaaa gacgagctgc cggccatcgg taatcaaatc
     3241 ttcttctccc gaaatggcta cggctccgcc agtccagcgg gctacagcga cggcagccgg
     3301 agcggcagaa cgaacagaat cggtagaatc gacaccgcga acgtccgaag tatcagaacc
     3361 gccttctaag ccccataatc cggctaaagc aatgatttca gatgcgttgc cccggataat
     3421 agacggttta taggccttga aggtcgtcaa aagttccgtc cgcatttgtc ccatgccaat
     3481 ggccacggga tccaggaccc agggattgcc ttcttcatgg agtaagactg ctgttattgg
     3541 cagtgtttcc gcataaatcg ggaataacgt ccctacgt
//
LOCUS       DAWCYS010000171         3564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1393_length_3564_cov_5.039612, whole genome shotgun sequence.
ACCESSION   DAWCYS010000171 DAWCYS010000000
VERSION     DAWCYS010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_3564_cov_5.039612"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          756 a         1087 c          996 g          725 t
ORIGIN      
        1 acccatcatc agtaccctct tctgccttgt tctgttattt tacgtgattc ggggacgtgg
       61 tagatcacca ggtttataga tgtcggcagc ggcgaaggcg tcgaaaaaga catgccggtc
      121 atcacgccga gtggtgtcgt cggcttcatc agcgacgtct atccgcattc gtcccgcgtc
      181 cagctcctga cggacccgcg gaccagcatc ggtgccatcg tacagcgtcc ggaatcgcgc
      241 gtttcctctg tcgtccgcgg caacggcaac gtgccgacgg aaccgcagtt cgtcaacatc
      301 gccaaggatg cggatatcct ggaaggggat acactggtca catcgggctt cggctccatc
      361 tatccgaagg gcctgcctat cggcaccatc gtatcgatcc atcagaatga aaatgatttc
      421 gtaaaatatg ccgtcattcg tcccagcgtc gatttctcca aactggaaga agtcttcgtc
      481 atcttgaagt cggccgatac cgggtcctat gaaaaaccgg aagtcacgcc gaaactcgta
      541 cctcagacga atcgcgacaa agtagaagga attaaaggag ccgcggcatt atgaagaaac
      601 tgaactgtgt catgacagcc ttcatcgtct ttattttgca ggctgccatc ctgcctttta
      661 tgtttaacag cgtgagccag cctaacctca tcttcgtctt cgtcgtcctc atggccctcc
      721 atcacggcca gcgcatcggc atcatcacgg ccctcttggg cggattttgt caggatgtca
      781 ttatcggcaa tttctttggc ctgcacctgc tggtctatct catcgtagcc ctcatctgca
      841 gctacctcgg ccgcgacatc gacaaggacc agtgggtatt gacattgctc atcgtcctcg
      901 gggcgaccga agtcggcctg gtcctgacct gcgggttcct cttcctggcc ggccagtaca
      961 tcaacatcgc cgcctacttg ttggaattca gcatccctat gcttgtttat cacggaatcc
     1021 tggccctgcc ggtggatcac atcgtctggc atctgcgccg cgacgatgcc ttttacgggt
     1081 tcccgggcta tcgctggtaa ataaaggaga tttagttaca tgcttgaagc gttattgaaa
     1141 aagaagaatc aagatggccg tttccgctac ctctactgga tcgtcatcgc gattttcgtc
     1201 atcctcatca gccggctggt ctacttgcag ctctttgccg gtgaatatta ttataccctg
     1261 gccgaaggga accgcctgcg ggccatcccc attgcagcca tgcgcggtat catgtacgac
     1321 cgcaacggcc agatcctcgt cggctcgcgg ccgagcttca tggtgaccta cgtgccgcct
     1381 aaggacggca tgagcgacga agaactgcag aaactggccg gcatcctcaa catgccggaa
     1441 gataagctgc gggaaaaggt ccagaaggtc aagagctctt acgtgccgac ggtcctggcc
     1501 aatgacttga cccaggatat cgtcacgaaa atcgaagaac agcgcaacga cctgcctggc
     1561 gtctccgtcg acgtccagcc gatccgctat tatccgtaca agatgatggc cgcccaggtc
     1621 tttggctacg tcggccagat cgatgaagaa gacgccaagc gcctggaagg cgtcgaaggc
     1681 gtatccaaca tcacccagat tggccgggcc ggcctggaag cctattacga tgacctcctg
     1741 cgcggcaagg atggcggccg tcaggtcgaa gtcgacgccg ccggcagtcc ggtcaaggaa
     1801 atggaacgca aggaccccgt agccggtcac aatatgcacc tgaccatcga cctctacctg
     1861 cagcaggcta cggaacgggc catggaccgc cagatcgcca acggcatcgg cacccacggc
     1921 atcgctgccg tcgccatcga ccccaatacg ggcgccgtcc tggccatggc cagccgtccg
     1981 gcttacgacc cgaactggtt tacccggggc attacggaaa aagaatggtc cctcatcaac
     2041 gacaatccca atcaccccat ggaaaaccgc gtcattgccg gcgaatatcc gccgggctct
     2101 acgttcaagc tgatcaccgg ttcggcagcc ctggaactca agaaagtcac gcctgatgaa
     2161 atgatcttcg acagtggccg ccactggctg gtcgacatgc gcaacgccgg cggcgaagcc
     2221 ctgggctgga tcaatttcgt cgaagccctg gctaagtccg ataacgtata cttctacgaa
     2281 atgggccgcc gcgtcggcat tgacgaactg gcgaaatacg cccgcatgtt cggcatgggc
     2341 aagaagacgg gcatcgccct gcgcggcgaa gcaccgggcc tggtcgccag tgcggaatat
     2401 aagaagaaga atttccacga tgaatggtac ctcggtgatt cgttcaagtc ggctatcggc
     2461 cagggggtcc agctggttac gccgctccag gcagccatga tcgtcagcga agtcgctaac
     2521 ggcggcatcc agtaccggcc gttcatcgtc agccgtatcg acaacctcga cggcacgccg
     2581 tataagattt ttgcaccgga acaggtcggc accctgtccg tatccaaatc gacgctggac
     2641 ctggtccgcg aagggatgcg taacgttgct gaagaatccg gtacagctgg ttccctgttc
     2701 gccggcttcc ccatccaggt cgctggtaag acagggacgg cggaaaactt cggcggtacg
     2761 gaccatggct ggttcgtcgc ctatgcgccg tatgaacatc cgcgcatcgt catcgccgtc
     2821 ctgacggaac agggcggttt cgggtctctg tcgtccggcc cgattgtccg ttccatgctg
     2881 gaagcctact tccacgtcgg tgaatacgcc gaaggcgcac cgaaggataa agacaaggac
     2941 ggcaaagaaa aggacaacaa ggccaataag aatgcgcagg acgggaagga cagcagccat
     3001 cgcgatgggg ctgagtctga ccgataagtc ctgatgacgg ccttatctca tcagaaagga
     3061 aagtgatttc atgtcccagc tcatcgccat cgcctccggt aagggaggcg tcggcaagac
     3121 cctgattacg gcggccttgt ctgtcctcct gcagcgccgg ggccaccgcg tcctggcggc
     3181 cgacgccgac atgggcctgc gcaacctcga cctgctcttc ggtctggacg ggtccatcca
     3241 ttacgatgcc gcccaggctg cgcgggggaa atgcctgccc ggccaggctt tgctgccggt
     3301 cgtgccggga ctggatttcc tgccggccag ccagaagcgg acctgggagc ggatcgacat
     3361 cccgtcgtat caatatgtcc tggagtccct gtccgggtcc tatgactata ccctcatcga
     3421 ctgcccgccc ggccgcgacg gcgcctatca ggcggcgacg gccctggccg atgagatcct
     3481 gttcgtcgtc gaaccgtcgc cgtcgtccct gcgcgatgcg ggtaaggtca tgcagtacgc
     3541 ggcccggcag aagcgctttc acta
//
LOCUS       DAWCYS010000172         3538 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1402_length_3538_cov_7.587425, whole genome shotgun sequence.
ACCESSION   DAWCYS010000172 DAWCYS010000000
VERSION     DAWCYS010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1402_length_3538_cov_7.587425"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          940 a          732 c          838 g         1028 t
ORIGIN      
        1 taatcttcgt tatgtgaccg ctcattgttt aaaaaaagct gactatgagc cccgcacttt
       61 gaattatatt acaagccagg aaaatccttt caatggaggc ctgtttccag aatcaggcat
      121 cacttgcgac ctctgtattg gcaacccgcc gtatatgaaa atcggaaaag atgcgcctga
      181 agctaaagcc atgccggcaa tttgttatgg tgctcctaat ttatattttc tctttgcggc
      241 tatgtcgtta tttaatgtcc gtgacggcgg agaaatggtt tacatcattc cccgttcttg
      301 gacaagtggc atgtatttca agcgctttcg ccaatattta ttcagccatg gtgtgattga
      361 gcagattcac ttgtttacca gtcgggataa ggtctttgat accgaacagg tattgcaaga
      421 aacgatgatt gtcaagatac ggaaacagcg tcaggttcct tcggatatat tggtcacttc
      481 ttctgaagct aattgtgatt tccgtcattt acagcagatt tcattgcctt attcggatgt
      541 agtttccgga tcggaccagt atgtcttttt gatgactaat catcgggaag tagacgctat
      601 ctgcttgtta aacacatttc cctataccct gccagatttc ggtatgaaaa tgaaaacggg
      661 attgactgtt gattttaggg agagggagtc tttgcggaat actgaagtcg atggggccgt
      721 accgctcttt tttgcccagc atatacaaaa cggtcatatt tgctttccga ttggtaaaga
      781 aggggaatat ttagtaccat cccggaaaag tttggtacag cctaatacta attatttgtt
      841 tatcaggcgg tttacggcaa aggaggaaaa gcgccgatta caatgtggta tttatttggc
      901 agaacaattt ccgcaatatc agaccataag cacgcagaat aaaatcaatt tcattgataa
      961 tgcggaccat acattgtcta aagatgtagt atatgggcta tatgttttgt tcaattctac
     1021 cctttatgat gtatattacc gggtactcaa tggttctacg caggtcaatg ctacagaaat
     1081 caatcatatg cctgttccag ctatttcgat tattgaagaa ctagggcgag cattgttgaa
     1141 gcaatccgat ataacagaaa aaacgtgcaa tcagatcttg gagggatttt tacatgggaa
     1201 aaatcagcga agctcaagcc attcttagag ctattgggat gcccgaacgc cagcaaacgc
     1261 ctatttgtgc gttgagtttg ctggctttgg ctcatatacg gcctgatgat gactggtgtc
     1321 aggctagcaa tgagtggatt cgtattcacg atataattca gttcgttaat ctgaattatg
     1381 aagttgatta tgcagagaat agtcgggaaa cgttccgaaa gcaggctatg catcattttc
     1441 gcaatgccgc tttgattgaa gataatggaa aagcgacgaa tagtcccaac tatcgctatc
     1501 ggttgactca ggaaacagtc cgccttttgc agacttttca caccgactta tgggaaaaat
     1561 ctttgaaaga atttactgat gttcacgaat cactgatttc catttacgca tcgaaaaaaa
     1621 aacgttcaga aaatgccagt catgattaat gagacggcct atactttttc accaggtaag
     1681 cataatgcct tacaaaaagc tatcttggaa gaatttgcac cgcgctttgc accaggggct
     1741 atttgcttgt atgttggtga tacgactaat aaaaacttag tggaaaacca gacgttactg
     1801 aaacaattag gaattgctct gacagaacat gataagttgc ccgatgtcat cctgtattgc
     1861 aaggacgtcg actggattta ctttgttgaa gccgtgacct ccgtgggtcc tatgagtcct
     1921 aaacgcttac aagaattgga agctatgact cagcaggtca cgagcgggaa aatctatgtt
     1981 accgcttttc ctgatgtaac cgtctacaaa cgttttgtgg cggacttagc ttgggaaacc
     2041 gaagtttgga ttgccgatga accagatcac atgattcacc ttaacggcaa tcggtttatg
     2101 gggccgcgtt gaatttataa aaggggctgt cgcacgaaga attttgtcat catgcgaggc
     2161 cccgtttact ttatagtttg tagcacagag tttgtagttt gcagagaaaa cctttaaatt
     2221 taaaactatc cgctaacccc gaaccactag ccactaacaa ctcaaaaagg gcttgtcgta
     2281 tgatgacaag cccttttttc acgcccagac gaatccggtc atagccatgg atcctaaggt
     2341 gcaggtatta ttgaatggtg tagctttatc cgttccggcg gcgcccaggc aatagagcag
     2401 gggcaggtag tgttccggtg tcggcacggc gtaggctgcg tgttcgtggt tttcgtagtg
     2461 gatgatgttg tcgatgttgc cggcgatgac attatcgacg atatcctggt tgaagtgttt
     2521 ggctgcttcg gagccgccgc gcttgttcca ttcgacgcgg cgcaggttgt ggacgatgtt
     2581 gccactggcc cagatgaggt agcctttttc tcggagcggg gcgagtttct ggccgagttc
     2641 aaagcattgg tccggtgtaa acgtgctgtt tacagaaagc tggacgactg ggatgggcgc
     2701 gttggggaac atgtggacca taacactcca tgttccgtga tcgatacccc atgaatcgtc
     2761 gatagaaacg tcattgccca aaagctggca gacgtcctgg gtcagttccg aacagccctt
     2821 ggctgggtat tggagctgat agagctcttc tgggaagccg tacatgtcgt agatttgacg
     2881 cggcttgtcg gcactctgga tatacgtgcc tgggacatac caatgggccg agatggccag
     2941 gatggctttc ggtctgtcct cactggttag aacgtctgcg ccgacgcgct gcatttcgcg
     3001 cgtcaagtcg ttttcatcga tagccagcat cggactgcca tgaccggtga agataacagg
     3061 cgtttttttc attatgatga atcccccttt gcatagaaac cataaagata aaaccatctt
     3121 tagtatatca tgaagttact ttacgtcaac aaactgccat ttttcacata tttaataatc
     3181 acgagggagt cgcaaaaatt tacgaatatt atagtgctgg attccatcct ggctaaagtc
     3241 gagttcgtcc atagaaacga cgtatttggg ataattgtct tttatgtctt tatatacacg
     3301 gaattcgcgt tcgatagttt ctggtgaagc gagtaaatat gtgacctgga cataacattt
     3361 tcgatttcct ttttcggcaa tgaaatcgac ttcaaaattt cctgttttgc caatcgttac
     3421 cgtatagccg cggcgcaata attccaagca gacaatgttt tctaatattt ggtcgagagc
     3481 gcgggcattg tggccatata cggcttctcg caggccatgg tcaacgacat agtacttt
//
LOCUS       DAWCYS010000173         3522 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1406_length_3522_cov_4.157485, whole genome shotgun sequence.
ACCESSION   DAWCYS010000173 DAWCYS010000000
VERSION     DAWCYS010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1406_length_3522_cov_4.157485"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          774 a          985 c         1052 g          711 t
ORIGIN      
        1 ggcagacgcc gataccgcag gccatccgct tttccatgga cacttcacag gcgacgccgg
       61 ctttggccga cgcctgggcg atggtcttca tcatgaccgt cggcccgcag accgatacgc
      121 ggtcgatggc gtgttctgca aagatctgcg gcagggcatc gacggcaaag cccttgatac
      181 ccagggagcc gtcgtcagtg gtcacgtaaa tctggccggc atagggttca aagaacttgg
      241 tccagaacgt ttcagccgcc gtcttgccgg caacgagcac gatgggatgg tccaggcgct
      301 tagccaaaaa gatgagcgga gccaggccga caccgccgcc ggccagcagg acctggcccg
      361 gcgttgtcgt aaagacgcct tcgcccaggg gcccttcgac gctcaatgcg tcaccgctgc
      421 gcaggccctt catttcttct gtgcccttgc cgaccaggcg gtaaatgatg gtgacatgac
      481 ccgtttcggc atcggcatcg acgatgccaa aaggccggcg caaaaaggtc tgtcccccat
      541 cgcgatggac attgacaaac tggccgggct gcgcttcccg ggcaatctgc ggtgcatgga
      601 ataccatttt ccatacttca ggaataatgg cttcattctc gacgatagtc gccatttcca
      661 agaatttctt cacctgctaa tcatccttcc tctgtttgca ataagaacag ttttattgta
      721 ccacagaacg gccgattttc gtagccaaaa tcagtgctgc cgctttttga cgatttcgcg
      781 gcgcatcttt tccatattgt cctgaaagac cgccttttcc tgcatggcca cgcgcgtata
      841 gaggacatcg ccgatggcca tgtggatcag gcgcgacgcc ccggcttctg acgtgtagtg
      901 gacttcgcgc cccatgccgc acaggcagat atcggctaac gcagacaaag gcgactggcc
      961 atggctggtg atgacgatga ctttggcccc gttctccctg gcggcctgga ccgattgcaa
     1021 tagttccttc gacgccccgc tatgggatac ggcgatgacc acatcgtcgg ccgtcatcag
     1081 ggccgccgcc gtgacctgca tatgggagtc ggcatactcc cggatagcca gtcccaggcg
     1141 caggaaacgc gtggccatat ccatgcagac cgtcgccgag ttgccaaagc caaagacgta
     1201 gatgcgccgg gctttcaaga gcagccgggc cgccgcatcg agagcgccat aatcgaggag
     1261 gctcagcgta tcctgcaatc cttcggcaat attggcaaag accttggccg ctacgtcccg
     1321 tccgctatcg ccggtctgga tgtcgtggaa ggccttctgc ggcgccgccg ataattccgc
     1381 agccagcatg agcttcaatt ccttcaggcc gctgcatccc aatttcttgc aaaaccggga
     1441 aatagtgatt tccgaactct gcgcatgaac ggccaggtcc gaaacggtct gttccatgac
     1501 ctcgtccgta tgatcgacga tataatcggc aatacggcgc tctgacttgg tcagaccgcc
     1561 gtatgcactg cgcaggacag gaaataactg gagtttcgtt tcttccattg tcttccctcc
     1621 cacttctgta tttactttta ttttagccta caaagatgtt caatacaagg acaccggcca
     1681 ggcccatgac ggcgatgatc gtttccaaag cgcaccacgt gcggaccgtc tgtttgactg
     1741 tcaggccgaa gaattccttg aagagccaga aaccggggtc attcggcggg ctgaagatga
     1801 gcgaaccggc gccggttgcc aggaccatca gttccggatt ggcaccggtg acggcgcaga
     1861 taggcgctgc gataccgccg gctgtcaggg ctgcaaccgt agccgaaccg caggccgtgc
     1921 ggatgacggc agcgaccagc caggccagga gcagcggcga taaatcagaa ccggcgacga
     1981 ggttgccgat atacgtgccg acaccgctgt cgatgaggac ctgtttcaag gcaccgccac
     2041 cgccgacgat gagcaagatc atagcaatgg tcaggacggc atcctggacc gtcttcatga
     2101 cgtcttccat ggatttaccc cgtcccaggc cgaacgtata gatagcgatg gcgacagaaa
     2161 tggtcagggc gatgtccggc tggccgaaga aggtcaggac gtgacgggcc tggacatcgg
     2221 cacccatagt caatttggca atcgccgaag cagccatgag gatgaccggg accagcgccg
     2281 tgaagacgga gatgccgaag ccgggcattt cgtcgtcttt aaagattttc gggttgtaca
     2341 tgcccttggg gatttccgga ttgatgtcgc gcgtcagcgt gcagtacagc ggaccggcga
     2401 cgatggccgt cgggatggcg atgatagcgc catagaccag ggtcgtgccg atatcggcat
     2461 tatagctgat ggaaatagct gtcgggcccg gatgaggcgg caggcagccg tgggtgcccg
     2521 acaaggccgc agccataggg ataccgactt ccagcagagg aatcttggct tctgcggcaa
     2581 tggtgaagac taaaggaatg agcaggacaa agccgatttc atagaacaag gcgataccga
     2641 cgatgaagcc tgtcaggcag acggcccatt tgacgttttc ccggccgaag acgttgatca
     2701 gcgtcgtggc gatgcgctga gcgccgccgc tttcagccat gagcttgccc agcatggcgc
     2761 cgaaagaaac gacgatggcc aggccgccta gggtaccgcc cagccccttt tcaatggtcg
     2821 ggatgagctt gtcgataggc atgccttcag ccaggccgac ggcgccggcg acgaggagca
     2881 aagccaggaa actattgagt tttactttga cgatgaggaa aaataagaca agaatgccaa
     2941 tggctaggat gacgagtggc ataatgagaa gcctccttta ttttgtatag tgctcgtgcc
     3001 aatgatgatg gaaagtgcct tctttatcga cgcgctggaa cgtatgggcg ccgaagtagt
     3061 cgcgcagggc ctggatgagg ttggccccga cctggggact gctgtaggca tcgaggtatt
     3121 gcagggctgc cgaaaaagcc gggataggca ggccgttggc gatggccagg ccgatgatct
     3181 gccgcagggc gccctgattg gcgttaatct ttgccaggaa gaagtcatcg aacaagagat
     3241 tgtccaggtc cggattcttg tcataggctt cggtaatctt ggtcaggaat tcggcctgga
     3301 tgatgcagcc agcccggaaa atagaagcga tagcgccgta atcgaggtgc cagtcatacg
     3361 tttccgacgc gctcttgtag agggaaaagc cctgggcata agcgacgatt ttcgccgcat
     3421 agaggctctg gcggacggct tcgacgaagt cgtcaccgtg ctgatgggac aaggcaggca
     3481 tttcgatgca ggcctgggct ttcgcacggc ccagtgcatt gg
//
LOCUS       DAWCYS010000174         3513 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1411_length_3513_cov_6.236553, whole genome shotgun sequence.
ACCESSION   DAWCYS010000174 DAWCYS010000000
VERSION     DAWCYS010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_3513_cov_6.236553"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          618 a         1065 c         1045 g          785 t
ORIGIN      
        1 ccggggcatc ctgcagggcg ccctgtcgtc aggaggcatc atcggccgcc tcttcggcgg
       61 cagcctggcc gaagccttcg gcatgcgcat gtccttcttc ctggcagccg gcgccctgtt
      121 cctcaacttc gtcgtcctgg tcttcttcat caaagaaccg ccgacggata cagccgcagc
      181 ggatacggcc gcagccgggg cagaaggcga aaaggtcagc ctctggaaga ttcccgtcat
      241 ccgcctgatg ctggtcagtg ccgccctggt ccaggtcgtc atcctcatcg tccagcccat
      301 attgacgaca tacatctccc acctggccgg cgagctggac aacctggtct tcatttccgg
      361 cctcatcttc tccatgagcg gcttttccag cgccattacg gctccgctct ggggacgctt
      421 cggccagcat cacggcttcg tcaaggccct gcgcctgtcc ctggtcctgg cggggatttt
      481 cttcttcatc caggccatcc cggacacgct gtataccttt gcggccagtc agttcgccat
      541 cggcctcttc ttctccggca tctatccgtc gatcaacgcc atcctggccg aaaagaccag
      601 tgccagcatc aaagggcgcg tcttcggcct gatgttctca gcccagcagg tcggggccat
      661 gggcggcccc atcctgggcg gcgtcatcgc caccttcctg ggcatgaaat acgtcttcct
      721 ggcagccggc gccctgctgc tgttcatcag tatcgctgtc cagcgcaaag cccgccagct
      781 ccacgaagcc tagccccgcg tgccgcggtt atgggtgtaa ttgggcagga gcttcggcga
      841 caggatgtcg tcatcgacgc gggcttctgc ccgttcttcg tcgaaacggc ggacgtggtc
      901 cagcgtccct ttcccaggat gggccgtctg gctgtagcca gccgcttccg tcccggcaat
      961 gcggccgaag acgatgatat ccaggaggct gttgcccatg aggcggttcg tcccgtggat
     1021 accgccgacg tcttcaccgg cggcatagag gccgggtatc ttcgttttac tcttggcgcc
     1081 gatacagatg ccgccattct ggtaatgcag ggtcggatag accaggatgg gctgcttgcg
     1141 gatattgatg ccaaattttt catacatgtg gatcatggcc ggcaggcgct tttccatggt
     1201 cccttcgccg ttgatgaggt cgaccatggg cgtgtcgagc cagacagccg gtccgtggag
     1261 ggcatagacc cctttgtccc gttcctgaca ttcgcggatg atggccgctg cgacgacgtc
     1321 acgcgtttcc aggggatgga ggaaggcttc gccgttctta ttgaccagca tggccccgag
     1381 agagcggacc ttttccgtca ccagggcgcc gtagatctgc ggcggatagg cagcgcccgt
     1441 cggatggtac tggatggcgt cagcatagat gaggggcacg ccggcgcggt aggccatgac
     1501 caggccgtcg gccgtcgagc cgtagtgatt ggacgttggg aagccctggt agtggaggcg
     1561 gccggcaccg cctgtggcga tgatgacgct cttggcccgg acgacgtggt attcccgggt
     1621 atcgaagtca tagaggacgg caccggccgc cttcccttcg ccatcgagga ggagttccac
     1681 agccggtgaa tattcgacga cgtcgacgcc ggcattgcgc acttcgtcgc ggaggacgcg
     1741 catgatttcc gcccctgtat agtcgccgca ggcgtgcata cgcttgcgcg acgtgccgcc
     1801 gccatgggtc gtgaccatgg agccgtcgct gttcttgtcg aagaggacgc ccagcgagct
     1861 gagccactgg atggcttccg gcccgtggag gacgagttcg gccagcagtt caggcttgtt
     1921 cttataatgg ccgccgccga tggcgtcgag atagtgggcc tccggcgaat cgctttcctt
     1981 gtcggctgcc tggatgccgc cttcggccat gcccgtattg gcatcgccca tgcgcagttt
     2041 cgtcgccatg aggaccttgg cgccgtgccg tgccgcttcg atggcagccg ccgcaccggc
     2101 gccgccaccg ccgatgacca ggacatccgt ttcgaactgg atgtggtcca agtcgacgtc
     2161 ggccgtatcc atgcggctct tgccctgcag cagggcggcc agttccttcg gcactttatc
     2221 gcctttattg gggccgacgg acaggacggc aaagccttct tcatggtaat ccggatggta
     2281 ctggcgcagg agttccccct tctcttctgc cgtaaagcgc ggcagttctt cagacaaacg
     2341 gatattccgc gtagcttcga cccgcgcctg ggaagcccgc atttcttctg tataaatcat
     2401 gatgtccccc tatttctcga tatcccgcga ttcatataag cggcgtaatt catcgtcacc
     2461 ggctgccatc agatgctgga cgtcggccac gaactggcct tcccggatgg cctggacgcg
     2521 ctggtccagg tgcctgctct tcttggcgat gtacttgccc atgaggcgcc gtgccaggag
     2581 gccgacggca ccgtgactga tcttggccgg gcagctggca gagcagaagt tgcagcagac
     2641 acagcggaag gattccttgg cacagccggc atcatcaccg cgctgggcca gggcgatgta
     2701 cttcatgacc tggatgccct ggggacagtt ccgcgtacag gcgttgcagt ggatgcagtt
     2761 gtagatttcc ggatagagct ggccgacgac gttgccggct acgggcaggt cgtccatatc
     2821 gtaagtctgt ttcttgatgg gaaatgattc caggcggccg acgcacatgc cgtcttcgac
     2881 tttcgtctgg caggccaggg ccgaatgggt ctccgtctgg ccctgcaggc ggtagacgac
     2941 ggcacaggcc ccgcagaaac cgctgcggca gccgcagccg cggaccaagc ggaaaccgga
     3001 atattccatg gcccgcatga tcgtcaaatc ttcagggacc gaaaatttct tgcccagtaa
     3061 atagaccgtt accattttca taatgataaa aactcctttg tgaatgaatg gcaaaagagg
     3121 ctgtcgtact aagacagcct ctttttagtt gatagtttgt agtttttagt ttgtagtgaa
     3181 tggctaaaaa tttaaccctc aactactaca aactacaaac tttatactac ataacgatta
     3241 ttccagtact tccaacaggc agtcaccgct gatgatggcc tggtctttgg tggcgtagat
     3301 ttcgccgact gtaccgtcga taggcgacgt gatggtcgtt tccatcttca tggcttcggt
     3361 aacgaccaag ggttcgcctt tcttgacttt ctggcctttc gtgaccagga ctttgacgac
     3421 ggagccggac agggttgcac cgatgtcgcc ggctttgtcg ggatcggcct tgcggctggt
     3481 gacggacttc atatcgacgc tcttatcctg gac
//
LOCUS       DAWCYS010000175         3504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1415_length_3504_cov_4.072195, whole genome shotgun sequence.
ACCESSION   DAWCYS010000175 DAWCYS010000000
VERSION     DAWCYS010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1415_length_3504_cov_4.072195"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          640 a          968 c         1064 g          832 t
ORIGIN      
        1 gcatatttta cgatctggat gatatcatcg gctgtgagct gttttggcat gtactttccc
       61 agtccggcca tttcggcctt ctcttcttcg acgaggtcgc tgcggcctgc cttttcaaac
      121 tctgccaacg attccttgcg ctgtttcatt tctttggcaa tgacggcacc gacgccagcg
      181 tcatcgagtt cgcatttgcc gtcgatttcc gtattgcgga tggacgaacg gaccatgcgg
      241 atgacggata aagccgtttt accggcttct ttggctttca tggccgcttt catgtcctga
      301 agcagttctt ctttaagaga catgctatca cccttcttac atttgacgat tatgctctgt
      361 acttccgttt ccgtgctgct tcggattttt tctttctccg tacactcggt ttttcgtagt
      421 gttcacgttt ccgtacttcg gaaagaacac ctgccttttg gcaagaacgt ttgaagcggc
      481 gaagtgcgct gtctaacgtt tcgtttttac caactctgat ttctgccatt ctattatccc
      541 tccctccaag gtatgatagg gagtgctttt actgcacaca atgaaattat actcagatta
      601 gcgtcatttg tcaacaattt ctgaggttca gcgtataaat ttactgaata cataattgcc
      661 atatcctaaa ccgtcttcac tgaggcagca gccccgctcg gtgcgttcca ggaggccctg
      721 gcggatgagg ccgtcgatga caggaccgaa gtcggcttcg atggatttcc cgaagcgtct
      781 ggcataatcg ccgtaatcga tgccgacccg gcggcgcagg gccaggaagc agtaatcttc
      841 gacggcccgt ttggcgtcga tggcgacgga ttcggcgacg acactctccc tgccggcctt
      901 atggatatac gccgggatgt tggctatatt ggcccagcgc gtcgtcccat cgaaggaatg
      961 ggccgatacg ccgaagccga tataaggctc ataagtccag tatttgctgt tatggcgcga
     1021 ctcgaaaggc cctttggcat agctggaaat ctcgtagtga tggaagccat aaccgtgcat
     1081 ggcgtcgtgg acggccttcc ccatggcttc gaccgtcccg tcaggcggca ggtgccatgt
     1141 ccccttttcc aggcccgcct tgagctgcgt cccgtcttcg acgatgaggc tgtaaatgga
     1201 cgcgtggacc acgggcagct gggaaaggcg ctctacgtcg cggcggacgt cggccaggtg
     1261 ctggccgggc aggccgtaca tcaggtccag ggacaaatta tggatgccag ccgcgtaggc
     1321 cgcctggatg gccgcttcgc cctgggctgc cgtatggacc cggcccagaa aggccagcat
     1381 ggcgtcggaa aaggactgga cgcccagact caggcggttg acgccgcaat cggccagagc
     1441 cgcggcctta tcataatcga gactgtccgg attggcttct atcgtgattt ccgcatcgct
     1501 ggcaatgcag aaggtctgcc gcagggtctt caggatgcag ctgacgtcgc cggcttccag
     1561 cattgacggc gtaccgccgc cgacgtagac cgtatcagcc ccttccccgg tatagggcga
     1621 ggccgcgatg tcccggcaca gggcttcgac atagccctgg cagtatcgct ccaggccggc
     1681 ataggacgga aagtcgcagt acaggcattt atggcggcag aacgggatgt ggacgtacag
     1741 gcccagcgtc attcttcgtc gactttgagg acggccatga aggcttcctg cgggatttcg
     1801 acggtaccga actgtttcat gcgcttcttg ccttccttct gtttttccag gagcttgcgc
     1861 ttacgggaaa tatcgccgcc atagcacttg gccaggacgt ccttgcgcag ggctgccacg
     1921 ttttcgcggg caatgatctt attgcccaca gcggcctgga tggggatctg gaagagctgg
     1981 cgcggaatga ggctgcgcag tttttcgacc agggcgcggc cgcggcgggc ggcgaaatcc
     2041 ttgtggacga tgacgctcag ggcgtcgacg acgtcgccgt tgatgaggat gtccattttg
     2101 accataggcg aggcctggta gccgttgagc tcataatcga gggacgcata gccgcgggtc
     2161 gccgacttga ggacgtcgaa gtagtcgtag aggatttcac acaggggcag gttgtagatc
     2221 aaagaaacgc gcgtgtcgtc gaggtaatcc atggtgacgt actgaccgcg gcggttctgg
     2281 gagatttcca tgaccgtgcc gaccatgtcc ttggggatga tgatcgtcgt cttgaccagc
     2341 ggttcttcga tgtggtcgat tttcgtcatg tccggcatct gcgacgggtt gtccacttcg
     2401 accatggtgc cgtccgtgca gtagacgtgg taaatgaccg acggcgccgt cgtgatcagg
     2461 gacaggttgt attcccgttc cagccgttcg cggacgacgt ccatgtgcag gagtcccagg
     2521 aagccgcagc ggaagccgaa gcccagggcc gacgacgttt ccggttcaaa gagcagcgcc
     2581 gcatcgttga ggtggagctt ctccagggca tcgcggaggt tgtcgtagtc gttcgtttcg
     2641 accgggtaga ggccgcagta gaccatgggc acagccttgc ggtagcccgg cagggcttct
     2701 ttggccggat tgtcggccag ggtaaccgta tcgccgacgc ggcagtcgcg gacgttcttc
     2761 atgctggctg ccagatagcc gacgctgcca cattccaggg acgggacggg atgcatctgg
     2821 ggcaggaaga cgcccgtttc gatgacttca aattctttgc ccgacgccat catgcggatg
     2881 cgctggcccg gcttgacgct gccttccatg atgcggatat tggcgatggc ccctttatag
     2941 gcatcgaaat gggaatcgaa gatgagggcc cgcaggggct tgtccccttc gtcagccggt
     3001 gccgggatgc gctggacaat ggcttccagg accttgtcga cgccgtagcc cgtcttggcg
     3061 ctaatcatga tggcgtccga tgcgtccagg ccgatgacgt cttcgatttc cttgcagacc
     3121 cggtccgggt cggcgctggg caggtcgact ttattgatga tgggaataat ttccagatcg
     3181 tgttccaggg ccaggtagac gttggccagg gtctgggctt cgacgccctg cgtggcatcg
     3241 acgatgagca gggccccttc gcaggccgcc aggctgcgcg atacttcgta gttgaagtcg
     3301 acgtgccccg gcgtgtcgat gagattgagc gtatactgtt tgccatcttt ggcatcgtaa
     3361 atgagacggg ccgactgggc tttgatggta atgccccgtt cccgttcgag atccatcgta
     3421 tcgaggagct ggtcttccat ttcccgtttg ggcacggtgc cggtcatttc cggcatccgg
     3481 tcggccagcg tcgatttgcc agcg
//
LOCUS       DAWCYS010000176         3502 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1418_length_3502_cov_5.033652, whole genome shotgun sequence.
ACCESSION   DAWCYS010000176 DAWCYS010000000
VERSION     DAWCYS010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1418_length_3502_cov_5.033652"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          950 a          875 c          824 g          853 t
ORIGIN      
        1 tatacgccga actgcgtata ggtatccgcg ggtatcttac aaggccgttg cgccggaagg
       61 taatcgtgct caacgacaaa ctggacgaac gggaaatacc gattgtcgtt gctcatgagt
      121 taggccacgc actgatgcac ggagcggccg ggacattcca cgccgatacc gtgaactatt
      181 gcaacgcacg gagtgaatac gaggctaaca tgttcgcact gtatcttcta tcctactgct
      241 acgatataga cgaacggctc ttacaggccg cgcccaggaa ccgggacgtg atgacatata
      301 aagaagccca ttcattattg tgcagatgta tcgagggata aaggagcgat aaaaaatgaa
      361 tcttatatta tgggtcgtgt tatttgctct cattggtctt gggaaatacg ctataggaga
      421 aataaaacaa aaccacaaaa caaaaggaat tgcactgctg attgttacga ttgccctcat
      481 gattgcatgg ctcgctttga aagggcacat atcccgcaat ataacgtatt agttatcatg
      541 tatagaaagg cggtaatatt atgaaaaagc tactattgat gctaatcact ttattggcat
      601 ttagttgccc tgtattcgct tcgcattgga tgcctcttgg aaccggggaa gatggtacag
      661 aatattcaat agacatttcc agggtgtcga cgcacacgtt aaccccgcta gaagaaggcc
      721 aaaatcgtgc cgtagaacga atgggataca aaggtattaa tgtcccatat aaaagcatat
      781 ggctacaagt agagtacacg gatggtactt cgtcgattca acacataaac gtatatcaga
      841 atcgaacaat agctctgctg ggatttgtgg actatgacag cgatggaagg ataaccaatt
      901 ctgcgcccga taggtaccca gtaccagaca cgatttaccc agatacagtc ggcgaaaaac
      961 tatatatgtt tgcatacccc atgaactcat aggcacgcaa caaaaataag cccacatgga
     1021 taccatgcag gcttattccc gattccaaca gggatggaga cagcggccag cccccaggag
     1081 caagggaatt ctaacccata ccgcggctgg cagatggcgg cagggctact cccattccgg
     1141 gaacccagct ttgttccttc acaagctggc ctatctctat tatatcattc ttaaaaatca
     1201 gaatctagtt tccttgcgta gtgcaggagt ttctttacac tgcagatttc gtcgagtagt
     1261 tgttcaactg ccgcgtcctc tccctgttcg tcacggagca tgatataaag tacgcgatag
     1321 aagttgtcgg ccgcttccag gtgcaggatg gccttcttga tatgtgcctc gaattctggc
     1381 gtccagtgtt ccgcgcggta tgccttttcg gtttcatccc agcccatatc taagtagtag
     1441 cgtgcgtttt catacagcat cgtgttccgc ttttcgggcg tgtcgacgaa ttcatataca
     1501 tcctcgtcgt ctgtcggctt gatcgcgtac tgattcccac tttgtttcat cttcaaatac
     1561 atttccatgg tttatctctc ccttcactat atagccacgg cgggcaatgg aatctgacgg
     1621 ttccagcaat atttttccgc cttcactagt aataggacag aaagagccat ttcaagtacc
     1681 actttttcaa aaaaatgtaa cgaattcaat tccgttgcaa actcgttccg catttacggc
     1741 tagtcaatcc aggaggcagc tatgtataac atcaatcaca actttacata ccgtcagaag
     1801 gataaaggct ggcaggtcat actttcttac aaggacggtg ataaatggcg ccaaaagagc
     1861 aagcagggcc taaagaccaa gaaacaggca aaggccgctg gcgacaaact tttatctgaa
     1921 cttcaaagaa catttgtacc ctcatcgaat gagctggcag gaatcacact ccgtaacttt
     1981 gtccccgtcg tcatcaagga taagacattg acgccatcga caaaatatgc attcagccgc
     2041 gtgccgattt tctttgctga tattgctgat aaggtcatct cccagataac gacagccgac
     2101 atactgcatg tcctgtctac aaagaagggc ctggcaaata gtacgatacg tcaccggctg
     2161 accttgttga acaccgtatt caaccacgct atccaggtgt acggcattgc gataaggaat
     2221 ccggtgcagg ccgtgccgcg cgtaaagctg ccgaaacagt cctacatcaa ggctatttcc
     2281 cacgatgatt tcacaaggct gatgaaaagc aacggcggcc ggaattgcga gaattacaaa
     2341 atttgttgcc agattgccta ttatacgggg atgcgattcg gtgaaatggc cgggctgata
     2401 tggaatgata tttcctttga tagcagacag ataaccgtta aacgacaggt gaaggcatat
     2461 cgccatgacg gcataatcac gtacgggcca gggcctttaa agacgacgaa ttcctaccgg
     2521 acgattcccg tccccggagt cctgcttgac gcactgaaag actggaaaca gaaaagcgcg
     2581 tcagaatcgg ttctaggctt aacgagttcc aacacgcaat taatcaaccg ttggataaag
     2641 aaaacactcc caaatacgag catacacgat tttaggcaca catacgccac aaacttactg
     2701 gcaaacggcg tagacattca aaccgtcgcc gcactatgtg gggactccgt gggaaccatc
     2761 acttccacct atcttgattt cacggaagaa atgagaaaga aggccgccca aaacgtcgat
     2821 accatctttg cgaaatgatt tttgacgaat ttttgacggc cctctatcgg gatgctatga
     2881 aacggcacta ttaagccatt cttcgtttgt tcggttataa caataaatta tatcatagaa
     2941 tatccaaatt ttatagtctt ttagtcgtca aactcctatt tttccgggct gccgttgacg
     3001 gcctgccaga cctgggcgac gaacttgccc gctgctgggg acatttcctg gatgtctggg
     3061 acggcgatgc ccagcgaaat cgactgggcc ggggccagag gcctaagttc gacgcggcca
     3121 gcccacttct ccgtcgtcag gcggttgttg acgctgattc ccaaaccctc ttcgaccatg
     3181 cagtacgccg tatacgggtc ggtcgtcgtg aagcggacat cgggctggac ctgattggct
     3241 gccaggaagc ggtcgatatc cgtatcgtga tccggcagag ggatgataaa aggcgccccg
     3301 ttcagccatt ctacgggaat ctccttttcc gccgtccagg gatgatccgg cggcagccag
     3361 acgacgagct catcgtcctt caagggaatc cagtcatagg ccacatccgc tccggccttg
     3421 gcggccagcc agcagtcgac ggccttctca ttgagccact gggcgcaatg ctgattcgtc
     3481 ccttcgaacc aatggacttc ca
//
LOCUS       DAWCYS010000177         3454 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1436_length_3454_cov_4.959400, whole genome shotgun sequence.
ACCESSION   DAWCYS010000177 DAWCYS010000000
VERSION     DAWCYS010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1436_length_3454_cov_4.959400"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a          927 c         1026 g          767 t
ORIGIN      
        1 gttgccaaaa aacgtccgga cgggccggcg cctatgctga ggacccgttc gaccatctac
       61 gtgtcgctgg ggacgaagtt ctcactgaat ttctcgaggg ccttggggtc gccgatgaaa
      121 tacgcattgt cgtgcgggaa caggcagtca tcaccatgag ggatgatcat gcggtgatcg
      181 cggaaaatca tgccggccag gatggacggc ggcatgttca tatccttaaa ggggatgttg
      241 atgagcggcg aatggcggcg gaccttggtt tcaaagagcc ggaccttgcc gttggcgaag
      301 tcttcgacgt tgagggcggc cggcgtcatg aggatgcggt tgatttcacg ggccgtgatc
      361 atttccggat tgagcatgag gtcgatgtcg aaattctgct tcagataatc cttggcttcc
      421 gacaggaact gcatgtcccg gatgcgggcc gccgtgtact tgatgccgtg tttcttggcc
      481 atgatacagg cgaccatatt gacttcatcg acagccgtac aggcgacgag gatatcggca
      541 ccgcggatat cgggatcgtt catggtaatc gggctggccc cgttggccag gacggtcaat
      601 acatcgagat tattcttgac gacttccagc cgttcttcat tatgatcgac gacgacgacg
      661 tcgaattgtt cattcgataa gagctcggca atactgtacc ccagcttgcc ggcgcctacg
      721 acaacgatac gcaaaggaaa gacctccttg taagtcgaaa tagaaaggag gctgccacac
      781 gaagacagcc tctttttata ctttaagttt gcagttttta gtttgtagtg aatgactaaa
      841 aattaaaacc catcctctac aaactacaaa cgctatacta caacttttga agacaaaggg
      901 ctccacatga cggcacaaac caccatgttc cagcccctac aaatagtatc acattttgtc
      961 ctttttgtac atctttcctt ttcgccgcca aggcgatgat ctggcagccc tctccccttt
     1021 gccccgagcc agccaacact caccctcagg cccgcggcgc caggtgctgc ttctgttcgt
     1081 acatcttggt gtcggcccga ttgaagacgg cccggtaggc cgtgtcgcgt gtcttgtcgt
     1141 agacggccat gcctacggaa gcggcgagct gcgtccacgg ctgagcggca ctcaggtcgc
     1201 ggatgtgttc aaaggcacgg actttctcga ataaggcgtc ccgatgagca tagtcctcgc
     1261 cttcgaggat gacgacgaat tcgtcgccgc ggatgcggta gaccgggctg ggcttataga
     1321 ggcggcagac ggcctgggac atgcgctgca tggcgatgtt ccccttttcg tggccaaaac
     1381 ggtcgttgac gtccttgagc tggttcaagt cgaccatgac cacggcaaag gcagcctggc
     1441 cggcggcgat tttctggttg aggtcgtaga ccttcctgtt gtaggcggcg acgttcttga
     1501 cggccgtcag ttcatcctga taggcctgtt tctgcatggc gccgacgtat ttgcgcatcc
     1561 gttccatcgt atcgttcata tggcctgcaa gccgcccgat ttcatcttga gctgtactgt
     1621 ccagaggctg gtcatattcg ccgcggccca ggcggcgggc ggcttcactg atgtcatgca
     1681 ggggccggac gatacggttc gccgcccgga tagccaggag cgtcgacaac aggccgatga
     1741 cgacggtcac agccaggatc ccgaaggtca tgagctggcg gccggcatag atttcccgtt
     1801 ccggtgccgt cacgcccagt ttcatgccat tcttcaaggt cgcaaagccc atggccatgg
     1861 gaatgcccag atagtaataa tgaatcaaat gggtatccgt cgctgactgc tccagcagct
     1921 ctccgtggct gacgatatcg aagcgcggct tgaactcgta ggccacctcc ctgccatcgg
     1981 gatggaaata aaaagtctgc cagtccttga agaggaagcc gtatccgctg tcatagacct
     2041 tcatgctgcc gatgaggtcg cagagcatgt tgaagtcgat atcgacgccg acgacggcga
     2101 cgaactggcc ttggacgtag acgggcatga cgtagctgat tatgcggaca ccattattgt
     2161 cattgacgta aggtgtcgtc cacaaggccc tcttctcacg caagagctga taataccagt
     2221 tcgtccggcc cgcgtcggct tccccgtacg cctcgacgga ggtaatgggc tgggacacaa
     2281 aatcatgacg cgcatctttg cgggtatacc agaagccgtc cggcccgccg gccaggtctt
     2341 cattgtaatg gaggtaatag ccctggacgc cgtcgatgcc ggatacagca ttataaaaag
     2401 accggctaat catggcttcc agccggtcgc ggttggcttt attacgcaag tcctggggat
     2461 ttttgacttc atgctcgatg gtatggccga tatagtggac gatgtcttcg gactgcatga
     2521 aaattggatt cagtgtcgta ctggcgtgga tggcccggcc agtgagggtc ttggcggcga
     2581 tttccgtctc gctgcggtcg acgaggaaga cgcagaggct gccgaagacc agggtcagcg
     2641 tcagggagac gccgcagatg acggtcaaaa agcgggaccg tatagtttgc atcatgaaag
     2701 ggtccctcct ttatgcagct gacaggccgg gcagcagagc gccggccgca cacaggccag
     2761 ggccaatttc cgaccgctcc ggggaacggc gatgtacgtg acctctttcc acgtatagcc
     2821 tgcatccgct cctttctggc ggaagcggaa gcaatccgac tggtcccctt gggataattt
     2881 ccttttgagc gccgtataat cggtgaaatc caggaagcgc tggcagtcat cgggatggat
     2941 acagctccgg gcatagtgct gaaggaactg gcgcatgaag aggcatttct ccatgagttc
     3001 cccggctacg gcatggcgcg agaacaaggg gatcccggct gatttgtcca agtcgatgag
     3061 gtagatgaca tcgtacaggc ggtagagctc atgaatgtgg acgttcagcg cctcctgctc
     3121 ctggatgtgt tcgtccaggg tcaggttgtg cagggacacg gtcagcatcc agcacgacgg
     3181 catggaggca acgagttccg ccgtgagctg gacgtagtag cccttttccg actggaagag
     3241 cgtttccaaa tgaccgctct gcttgaggcc ttccatgagg tcccggaaat gggcataaag
     3301 ccgcgttcca ggccggttga tgcgcagttc ggcactgagg gccgtaatgc ggccgaaact
     3361 ggccagctga tcccgatagg cctgattgag atagacgtga tggaatgctt ggccgtcgta
     3421 atcgaccagg gccagggact gatgggtccg gcgg
//
LOCUS       DAWCYS010000178         3449 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1439_length_3449_cov_6.555687, whole genome shotgun sequence.
ACCESSION   DAWCYS010000178 DAWCYS010000000
VERSION     DAWCYS010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_3449_cov_6.555687"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          708 a          995 c          959 g          787 t
ORIGIN      
        1 accgacgacg gtgtaatcga agcggtcccc cgtttcggca tcttccagga tgactttgga
       61 accgaggcgg actttatccg agccatcatc ggtttccttg atgatcttag ccgtgcggat
      121 cttggcttcc aattcggcaa tacggccttc gatgatgccc tgttcattct tggcggcatc
      181 gtattcggcg ttttcgctca agtcaccgag ggcgatagct tctttgatgc gctgagaaat
      241 ttcaatgcgg cggaccgatt tcagttcatt caattcatct tttaatttct gtaacccttc
      301 ctgggtaatc agcgtttcgt tggtcatgat attcctgctc ctcctgcgat ataatcacgt
      361 ttcaatatca tatctttaat ttataccgta ctcttaatta tatgcacatc cgtcccttgt
      421 gtcaataaaa cagctatgtc cctgcccggc gcgccttgtc catcatgtcg cggacgatgg
      481 ctcggaattc gtcagccgaa ccggcccggt tgatgcgttc gcgcagggcc gccgagccat
      541 agaggccgtg ggtataccac gaggcgtggg agcgcatctc ccggacgccg atgtattcac
      601 ctttataggc cagcagggct tcaaaatggg acagcatctg ttcataacgt tccatggccg
      661 tcggcggcgg caggacttcg cccgtcgcca aataatggcg tatctgcggg aaaatccagg
      721 gattgccctg agcgccgcgg ccgaccatga cggcaccgca accggtctgt tccatcaggg
      781 ctgccgccga cgggccgtcg acgacgtcgc cgttgccgat gaccgggatg gatacggcat
      841 cgacgacggc tttgattttc tgccagtccg ccttgccgct atagaactgt tcccgcgtcc
      901 gtccgtggac ggtgacggca gcggcaccag ccgcttcagc ccgtttggcc agttccggcg
      961 ccgtaaaaga ggcgtcatcc cagccggtgc gcatcttgac cgtgaccggg atggacacgg
     1021 cgccgacgac ggcacggatg actttctctg ccaggggcag gttcttcatc aaggccgagc
     1081 cgtcgccgtt cttgacgact ttcttgacgg gacagcccat attgatatcg acgatatcgg
     1141 caccggcttc ttcgacgacg gccgctgcct gggccatgac gtccgggtcc gagccgaaaa
     1201 tctgcatgga cagggggtgt tccccgtctt cgatgtgcag cagttcttct gtatgctggt
     1261 tgcggtagcg gataccctgg gaactgacca tttccgcgca gaccagggcc gccccatagc
     1321 ggtgggcgat gacccggtac ggcgcatcac agacgccggc cataggggcc aggatgaccg
     1381 gggcatcgag ctcgaccttg ccgatggcaa agggcttaac ggttgcggtc ataaatgatt
     1441 ctcaaccctt ccagggtcag catgggttcg acgacgtcga tggcatccgt agccggagcg
     1501 acgatgacgg ccaggccgcc cgtagcgacg actttagcct tacagcccag ttccttgcgc
     1561 atacgggcga cgatgccgtc gacttggccg acgaagccat agaagacgcc ggcctgcata
     1621 ctttcgacgg tattgcggca gatgacctta tccgtgcgga cgacttcgat acgcggcagt
     1681 ttcgccgtgc gctggaccag ggcgtccgta gagatgccga tgcccgggca gatggcgcca
     1741 ccgaggtacg tccctttctt gtcgacagca cagaaggtcg tcgccgtgcc gaagtcgatg
     1801 atgatgaccg ggccgccgaa tttttcatac gctgcgacgg cgttgacgat gcggtcggca
     1861 ccgacttcac ggggattgtc gtatttgatg tccatgcccg tcttgacacc cggtccgatg
     1921 accagcggcg tcaggccgaa atagcgctgg cacatgcgtt ccagcgtggg catgaccggc
     1981 ggtacgaccg acgaaatgat gatggcatcg atttcatcgg agttgacgcc gttcaaataa
     2041 aagaggttgc gaatgaggac gccgtattca tcggtcgtcc ggaggcggtt cgtcgatacg
     2101 cgccagtggt ccatgagctc ttttcccttg aaaatgccca ggacaatatt tgtattccct
     2161 acatcaatga ctaatagcat aactttatct cctgcctact ttaagttgat aatacgccta
     2221 ttataataac agagtttgcc tgtcttgaat agtctttacg gctaaattaa gccggacgga
     2281 catgaatatc ccctgccatg acgacttcca cgccttctgg cgtcgcgacg aggagattgc
     2341 cgttctcatc gatatcgacg gctttgccgg aaaaggtctt gtcgatggat gtgaccgtta
     2401 cgtcctggcc caacatgcag gacagggccc gccattcggc caggactggg gcgaaaccgt
     2461 cacggcagac ggtctggtac agcccttcga tttccttcag cagcgtttcc agcagggcca
     2521 gccggctgac gcggacgcct tcgcgggcaa aactggtgac gatggactgc aaatccttag
     2581 gcaggtcctc agcggtgata cccgtattga ccccgatacc catgatgatg taattgacct
     2641 tttccgccga accgtttagt tcggtcagga tgcccaccat cttgcggccc ttgaccagga
     2701 tgtcattagg ccacttgatg ccgcagtccg tcaggccgtg atggcggatg gcccgggcca
     2761 cggcgacggc agccaggagg gtcatcttgg agacttccat aggcgggatg ttaggccgca
     2821 ggatcaggga gaaccagacg cccttggcaa aaggcgacaa gaacgagcgg ttaatgcgtc
     2881 cccggcctcc ggtctgttct tcggcgacga cgatggtccc ttccggggcg ccttcatcgg
     2941 ccagcttctt ggcgacatta ttcgtcgaat cgacgcgttc ccggtagacg atatgacgtc
     3001 ccacgacttt cgtcgaaagc ccttcttcga tagcctgttc cgacagcagt tccggcgctt
     3061 cgcgcagaca ataccctttc ttggtatacg actcgatgac atagccccgc tgtttgagga
     3121 cgcggatatg cttccatacg gccgtccggg aaatcccgag ctgctcagaa atctgctggc
     3181 ccgatacgaa ttcgtccttg tgctgtatca aaaaatcgag aatgtcttgt ctcatagcaa
     3241 tcacctctgc cgctgcccct ggcagcatcc attcaaaaaa aagcgctact ccctagcgca
     3301 taactcacct attctatgta gaaattgtaa cataaacgat aaccctgtca aggggagatt
     3361 agggttagtg gctcgtggag agcaccacca aatcccggaa gtttgacact tgctgcttat
     3421 cccattgaat aagaaatcct ataaccacg
//
LOCUS       DAWCYS010000179         3442 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1445_length_3442_cov_3.948332, whole genome shotgun sequence.
ACCESSION   DAWCYS010000179 DAWCYS010000000
VERSION     DAWCYS010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_3442_cov_3.948332"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          810 a         1020 c          992 g          620 t
ORIGIN      
        1 cggcttcgtc gcggcgaacc tcgacggcgg ccggcgcctc cagtcccgcg tcaagttccg
       61 gtccctggtg acattggatg cggatacgcc agctaaatcg tttaagaaag acctggaacg
      121 cgtgcttgca ggccgagcct acgtcttata ctccacgcac tcccacacgg cagaggcgcc
      181 gcggtaccgc gtcctgatac ccctcgatac ggatgccgaa ccggatgcct accaggccat
      241 cgcacggcgg ctggcgtccg acatcggcat gcagcacttc gacccgtcga cgttcgacgt
      301 gtgccggctc ttctactggc cctcgaaacc gcgcggcggc ctctacgagt accacaacca
      361 gccagggacg ctgctgagcg tccaggatgt actggcgacg tacgtcgact ggacggactg
      421 cacggcctgg ccgacgagcg cgaccgaaga catcgcccgg aagcgcgaca tcaagaagca
      481 gggcgagccc acggaaaagc ccgggttcat cggcgccttc tgccgtgtct acacggtgca
      541 ggatgccatc gcgaagtatc tcccggacgt ctatgagccg acggcggacc cggaccgtta
      601 cacgtacgcc ctgggctcca cgacgggcgg cctcgtcatc tacgacgata agttcgccta
      661 cagccatcac ggcacagacc cggcgggcgg cgtcgactgc aacgccttcg acctcgtacg
      721 gctccagctc ttcggggtcc aggatgagga catcgaggct gactgcccgg tcaacaagcg
      781 cccatccttc aaagcgatgc aggacctggc ggcctcggac gacgcggtcc ttttagaata
      841 taaccagcac aagacccggg agctgcaggc cctctgggac gataaattga tcgacgagga
      901 cggcaaaccc gccaaggtcg acaagagctg gatgaaaggg ctcacggcgg ccacgggcaa
      961 gcgcggcggc tacgagtcgg acagccgcaa catcaagctg atcctgatgc gtgacccgct
     1021 gctggcaggc cttgcggcct gggacgagtt ccagagccgc acggtcgcca ccggtgacct
     1081 gccgtggcgg cagcacgcca agaacacgat atggtccgac acggatgacg cctgcctgcg
     1141 caactacctg agccagtact atcagataac cggcaagcag gtcatcgacg acgccctggc
     1201 cgaggtcacg cacatcaaca cgatacatcc ggtacgggaa tacctggaca gcctcgtctg
     1261 ggacggcacg gagcgcctcg gccggctcta cgtcgatttc ctcggcgccg aggacaccga
     1321 gtacacgcac gcgctgacca tcacgcacgc caaggccgcg gtgcgccgca tctatgagcc
     1381 cggctgtaaa ttcgacaacg ttctcgtctt acagggtccc cagggcatcg gcaagtcgac
     1441 gatcatccga cagatgatgg gcccctgggt caacgactcc atcggggaca tcacggggaa
     1501 ggatgccatg gaaggcctga aagggtcctg gggcgtcgaa ctgtcagaaa tgcaggcgac
     1561 caaccgcgcc ggcaccgacc agatcaaggc ctacatcagc aagaccgaag acaagttccg
     1621 cttggcctac ggccggcgca cggagatttg caaacggcag tgcgtgttct tcgcgacgac
     1681 gaacgacgac atctgcctcc gggaccgcac gggcggccgc cgattctgga tcctcgagtg
     1741 ccagggcggc acggcgcggg gcccgatgga catgacgcag gacgaaaagg accagtactg
     1801 ggccgaagcg gtctgcctct accgggagaa cccgagcctg ctgctaccgc ccgatatcga
     1861 acggcaggcc cgcgacgtcc aggaagcgaa gaccgaaggc tccgagctcc agggcgtcat
     1921 cgaagggtat ctcgacatgc cgatcccgaa gaactgggac cggatgacgg tatccgagcg
     1981 ccagcacttc atcctgtacg gtgatgaggc cggggatctt gcggatggga aatacttaca
     2041 ggtccgtacc tgtgccctgg cagtgtggtg cgagtgccta ggcggcaacc gggtcaactt
     2101 caccaacgcc aaagcccgcg aactgaacac ggtaatccga aacatgcctg gatggatacc
     2161 gtataaaggt aacaaatctg gcaaactgaa gttcaggaat tatggtgttc agcgggcgta
     2221 catccgcatt aaaaagccag aaaaatagtg gcaactttca ggttgtcata attggtaaca
     2281 tcgtggaaac ggtgaaaacc aactttattt atgattatca attctaaaaa gtgaaaatgg
     2341 ttgccgtagt tgcctaatat tgccgattct tttgcaaata ggcaaccgtg gcaatccggc
     2401 actaccaagc cactcattaa tgttaagttt ccattgttgc caattattgt tataaaagtt
     2461 taaaaagggt atatagacgc gcgagagagt tttagtaaaa aatccgttga taaaatactt
     2521 tcaaaatgca ttgtctaatt ccctattttt aagctaaatg ggaaaaatag gcaactttgg
     2581 caattttcgg ttgatttgca gggaggagat tgtacgtaac acatggacat taacgaaaaa
     2641 gacgtcgaaa ggtacctcgt ggccctttcg cataaaaata aaattatgac gttcaaattt
     2701 acgagcccga accagtccgg cgtgccggac cggatcgtca tcgcaccgga cggagcgctg
     2761 tatttcgtcg agctgaaacg gccgggacag cagccgcggc ccatccagca gagcgtcttt
     2821 gccaaattac gcaattacgg ccaccccgtc tacgttatcg acaataagga catcgcgaag
     2881 cggttcatcg acgacatcgt tcttaagcgg cggccgtgat gtggttcacg ccgcatgatt
     2941 atcagcagac ggccatcgac aagaccctgg agacaccgcg ctgcgggctg ttcctccgga
     3001 gcggcctcgg gaagaccgcc gtgacgctga gcgtcatcgc cgaactcctg tatgaccgcc
     3061 tggagctcgg caaggtcctc atcatcgcac caaagaaggt cgccgagagt acctggcagg
     3121 atgagatggc caaatgggac aacttcaggg agctgacggt ctcgaccgtc cttggatcgg
     3181 agcggcagcg ccgggatgcc ctggccgccg atgccgatat ctacatcacg aaccgcgaaa
     3241 atacagtgtg gctcgtggag cagtatcact atcagccacc cttcgacatg ctggtactcg
     3301 atgaatcttc gtctttcaag aatccccagg cgaagcgctt caaggcactt cggaagtgtc
     3361 ggagctgctt ttcccggatg atcctcttga ccgggacgcc gtcaccgaac aacctcatgg
     3421 acctgtgggc gcagttgtac ct
//
LOCUS       DAWCYS010000180         3429 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1454_length_3429_cov_5.463545, whole genome shotgun sequence.
ACCESSION   DAWCYS010000180 DAWCYS010000000
VERSION     DAWCYS010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1454_length_3429_cov_5.463545"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          779 a         1015 c          972 g          663 t
ORIGIN      
        1 cagcaattcc cagcccgtca gcagcggcgg gacgggagcc atgatctggc ccgtcaacgg
       61 cgtcatcacg tcgccttacg gttaccggac tcatcccatc tttgggacga ccatctacca
      121 cagcggcatc gatatcggcg tcgattatgg cacgcccgtc caggcagccg atggcggtac
      181 cgtcgtcgaa gccggctgga tcagcggcta tggctatgcc gtcgtcatcg accacggcaa
      241 cggcctgtcg acgttgtacg gccacaatca ggaactcgcc gtttcgccgg gccaggccgt
      301 ctctcaaggc caggtcatcg cctatgccgg ctcgacgggc aactccacgg gcccgcacgt
      361 ccactttgaa gtccgcgcca atggcgaccc cgtcgacccc caggggtatt tgtaggggaa
      421 agtcggcgcg gtacggcttc tgctgccagg cacgagtacc taatcgtttt tcgttcgtcg
      481 ttcgtcgttc atcgtgacgg gtaatttttt atcttttacg atcaacgatg aacggactac
      541 gatgaacttt ttttacacac aaggaggcac cctgatgggg aaacatagtt tacggcagac
      601 gtgggagagc gtccgccggc cgctgggata tgtctgcagc ggtatgatca tcatgattgt
      661 cgccatggtt ggcctctttt ggtattatac gggccggcct tcgtcgatgt tcgagttttt
      721 ccggaccttg gaaatcatcg aaagccatta tgccgaaaat gtcgataaaa acgccatctt
      781 tgacggtgcc ttgaagggca tggtcagtac tttgggcgat aaacattcga cctatctcgg
      841 cggcgatttg tataaagact tttccgccca gatgagcggc acttacgccg gcatcggcgt
      901 ctacatcgcc tctaccgatg aaggcatcct catcgccggt gtcatggaag gcagtccggc
      961 tgaggaagcg ggtctccagc gcggtgatat cctggtgtcc attgatggca cgtccgtcga
     1021 gggctatcag ctggaagatg tgtccaagaa catccgcgga cctgtcgaca ccagcgtcga
     1081 cctcgtcgtc cgccgcgatg gcgaagaaca atcctttacc gtccagcgcc gccagatcca
     1141 cgtgccgaca gtcgccggca agatggtcga cggcacggac gtcggctata tccgcgtggc
     1201 cgtcttcagt gatggcacgg ctgacgattt caccaaggaa ttcaccaagc tccgcgagca
     1261 gggcatgaac aagctcatcc tcgacctgcg tgacaacccg ggcggcatcg tcgaacaggc
     1321 tgtcggcgtg gccagcaatt tcgttccgcc caacagtacc atcgtgtctt atacggaaca
     1381 ggatggcaaa gtggaccagt acacggccca ggggacggat gacccgattc ccatcgtcgt
     1441 cttggtcaat gaaaattcgg ccagcgcttc ggaaatcgtc gccggtgccg gccaggacat
     1501 gaccctcgga cccatcgtcg gcgtcaagac ctaccgcaag gggaccgtcc agggcgtctt
     1561 ccccgtcgac tcggcatcag ccgtcaaagt gaccgtcgcc aagtatcgga cgacgaatgg
     1621 ccgcgaaatc gacggcgtcg gtatcgaacc ggatgtcgtc gttcccttga cgccgtccga
     1681 cccggaagac agccagttcg agaaggcttt ggaaattatt caggaaaaat gactttacag
     1741 atgagcgaat atgctttaaa atttgatagt aacgtcaatt agatattttt ggacagatag
     1801 acaggagaca tggtatatgt atgctgtgtc tctttctgta tccgcatgta ttcctatgag
     1861 gtgaaatatg tttatagaca gagcaagaat ttttgtacag tccggcaagg gcggcgacgg
     1921 catgagcagt ttccgtcacg aaaagttcgt ccccaagggc gggccgaacg gcggtgacgg
     1981 cggccagggc ggcaacgtcg tcctcgtcgc cgaccgcaac gtcaataccc tggtcgactt
     2041 ccggttccgc cgcctgttca aggccaagcc cggcggaaaa ggtgaaggca gcaacaagta
     2101 cggccgcaat gccgaagatt tattgatcac tgttcccttg gggaccatcg tcaaagatga
     2161 agaaacgggc caggtcatgg ccgacttatc ccgcgacggc cagcgggcca tcgtcgccaa
     2221 aggcggccgc ggcggccgcg gcaactggca tttccgcacc agcgccaacc ggacgccgac
     2281 ctttgccgaa cggggcgaac cgggggaaga acggtggctg cgcctggaac tgaaggtcct
     2341 ggctgacgtc ggcctcctgg gttaccccag cgtcggcaag tcgagtatcc tgcgcaaggt
     2401 ttcggcagct cagccggaag tcgcagctta ccacttcacg acgctcaacc cgattttggg
     2461 tgtcgtcaac ctgccggacc accgcagttt cgtcatggcc gatatccccg gcctcatcga
     2521 cggcgccagt gaaggcgtcg gcctgggcca cgatttcctg cgccacatcg aacggacgaa
     2581 gatcctcatc cacgtcgtcg acgtgtcggg catcgaaggg cgcgacccca tcgaagacta
     2641 tgaaaagatc aatgccgaac tggcgaaata cagcgaaaaa ctggcgcgca aacgccaggt
     2701 cgtagcggcc aacaaaatcg acctcctcgg cgattcggac aacctggaac ggctcatgga
     2761 ctacatgacc gctcacggcg tcgaagtcta tcccatctgc gccatgaccg gcgaaggcat
     2821 ggacaagctc ctggaacggg tctggacgat gctcgaagag tacgtcgaag aaccggacga
     2881 aacgacggaa gaagtcgtct ataaagccca gaataagccg gactttgaag tcaagcgcga
     2941 cgacgacggc gccttcgtca tcaccggcgc ccgcatcgaa aacctcgtcg ctatgaccaa
     3001 cttcgacgac gaccagtccc tgcgccgctt ccagcgcatc tggcgctaca tggaactgga
     3061 caagctcctc caggaacacg gcatccagga cggcaacacc gtccgcattt attccatgga
     3121 atttgaatac cacaaataac gcaggccgcc attggctccc ctggtagggg agccatcgta
     3181 ggggctcgca cgtggcgagc ccgcctgagt ccaacatatt ttacataata atgagcacaa
     3241 ggagatccat atgaacaata aagataagaa gcaactcaag ggcatggcca gcctcatgcc
     3301 gtctgtcgtc cagatcggca aggacggcct cagcgatgcc gtcatcgaca gcgcccgtgc
     3361 tgctattacg gcgcgggaac tgatcaaggc ccacgtcctc aacaacgcca atctgccgac
     3421 gaaggaaac
//
LOCUS       DAWCYS010000181         3397 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1466_length_3397_cov_6.876421, whole genome shotgun sequence.
ACCESSION   DAWCYS010000181 DAWCYS010000000
VERSION     DAWCYS010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_3397_cov_6.876421"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          809 a         1009 c          930 g          649 t
ORIGIN      
        1 ctacgactgt gcctactgtg tcaacgcggc cagccgggaa gcggaacggg ccatcctgac
       61 gccggaagag atcgccgacg tgaccatcgc cttttaccgg cgcaattaca tcgaaggcct
      121 gttcctcagt tccggcgtct atcgttcgcc ggactatacg acggagctcc tcatccggac
      181 ggcccgcatc ctgcgggacg aaaagaaatt caacggctac atccacatga agggcatccc
      241 cgggacgagt gcccagctca tccgtgaact gggccgctac gtcgaccgcc tgagcgtcaa
      301 catcgaactg ccgtcggcgc cgagtctgcg cctgctggcg ccgcagaaga cgaaggaaaa
      361 catcctcggt cccatggggc acattcgcca gggcatcctg gagcggcggg aagagaagaa
      421 acatcataag aaggcgcctt ccttcgtccc ggccggccag acgacgaagc tcatcgtcgg
      481 tgcgacgccg gacagcgacc ggacgatcct gcgcctgagc caggccttat acgataaggt
      541 ggaattgaag cgcgtctatt attcggctta tgtcccggcc gtgaccggcc caacctgccg
      601 gccatcgcca agccgccgct cctgcgggag caccgcctct accaggctga ctggctgctg
      661 cgcttctacc agttcaaagc cgatgagata ttgactgacg aggcgcctga tttcgacctc
      721 gacctggacc acaaggcctg ctgggccctg cgccatccgg agtttttccc ggtcgaagtc
      781 aaccgggcgt cgtaccacgc tttgctgcgc gtccccggca tcggcgtcac ctcggcccgg
      841 cgcatctgtg cggcccggag ccaggctttc ttgtcgtatg acacactgcg ccgcctgggc
      901 atcgtcctca agagggccca gtatttcatt acctgcaagg gaaaatatta tggagcgacg
      961 acggacccgg tccgcatccg ccagctgctc ctggcccggc cgtcagccca gacggcttat
     1021 gaaccgatga gtctgtttta ggagcggtag cgatgattta cagttatgat ggaacttttt
     1081 tcggctattt gtcggccgtc atcgaccggg acgaagcgct gctcagaaaa gaccaggccc
     1141 agccggctgt ccgggcagat atcttcgacc tgggccaggc catcgtgccg caacgaggcc
     1201 ctgcgccgca gttatatgcc caagaagtat tggtcttttc tgattgaaat gaagtgaaaa
     1261 aaagtatttc tttttcccag cacttgttat ataatagtaa cattggattt gtgataggaa
     1321 aggtggaata cattgaaacc tgtcttagta agtgtaaaaa gtatccaacg taacaaagag
     1381 ggagaagagc tgaaaatgga gctcgtttcc gaaggcaagt tttacgtcaa ggacgggacg
     1441 cagtacatcg tctacgaaga aagcgagctg acggaaatga agggcgttac gacggtcatc
     1501 aaggtgttac ccggagggtc cgtcttattg ttgcgccttg ggaagctccg ccagcgtcag
     1561 gaattccgca agggaaaagt gacgcgctcc cagtatgaca caccggtcgg cacgttcgac
     1621 gtgcagatga agacctatga atgccgcgcc gacttgctcg atggagtcgg tacgattcag
     1681 ttgggctacg acgtccatat cgaaggcgtc agtgccaatt atacacaatt aaccataacc
     1741 gtgcaggagg atcaagcgta atggaaatga aggatttatt gaagcagggc attacggaag
     1801 ccctgaacaa ggctattgca gccggcactc ttccagccgg cgactatccc gacgtcgcac
     1861 tggaagtacc gccgcagaag gaattcggcg acttcgccag caacattgcc atgcagtcgg
     1921 cccgcgtcgc ccataaagca ccgagactca ttgcccaggc catcgtcgac ggcatggatt
     1981 atccctggct cgaccatgcg gaaatcgccg gtgccggctt catcaacttc ttcttgaaga
     2041 atgacgtcat ttacgatacg ctcaaacaga tcctggctgc cggcgatgcc tatggccatg
     2101 caccgctgcg caaagaagac acgatccagg tcgaatacgt cagtgccaac ccgacagggc
     2161 cgctccacgt cggtcacggc cgcggcgccg cttatggcag tgccctggtc aatctcctgc
     2221 gggctgcggg ctataacgtc caggccgaat actacatcaa cgacgccggc aaccagatga
     2281 acaacctggc cgcttccgtc aatgcccgct acctggaact gttgggcaag ccggcagaaa
     2341 tcccggaaaa cggctatcac ggccacgaca tcatcgaaac ggcccaggcc atcatcgacc
     2401 aggacggtga caaatacctg gacatgcctg aagaagaacg gctggaactc ttcaaggacc
     2461 gggcctatgc cgaaaagctc aaggccctca aacgcgacct ggcccatttc aacgtccatt
     2521 atgacaactg gttcagcgaa cggaccctcc atcccgatgc catccaggca gcctgcaagg
     2581 tcctggaaga acggggtaag atttatgaaa cggacggcgc cctgtggctg aaatcgacgg
     2641 attacggaga cgacaaagac cgcgtcgtca tccgcgacaa cggcgtgccg acttatctgg
     2701 ctgccgatat cgcttatcat aagaataaat acgaccgggg cttcaagaag atgatcaata
     2761 tctggggcgc cgaccaccac ggctatgtag cccgtgtcaa agcggccatg gcggccctcg
     2821 gctatgacgt caatcagctg gaagtgctcc tcttgcagat ggtcagcctc ttccgcgacg
     2881 gcaaaccggt caagatgagc aagcgcacgg gccaggccat tacgctgaac gaactcatcg
     2941 aagaagttgg taccgatgca gcccgttact tcttcatcat gcgttccctg gatacgcagc
     3001 tcgatttcga cctggacctg gcaaaatcgc acagcaacga aaacccggtc tattatatcc
     3061 agtacgctca tgcccgtatc tacagcattt accgtcaggc caaggaagcc ggcgccgacc
     3121 tcagcacgga ctggtccgac gtcaaatggg atacgctgac caacgaatac gaactggaac
     3181 tcatcaagaa gatggccgcc ttcccggaag aagtccagcg tgccgcccgc gaacgggcac
     3241 cgcaccgcat tgcccacttc gttcacgaac tggccggtat gttccatacg ttctacaacc
     3301 actgccgcat catccaggaa gacaagaatc ttgaaagagc cagtatggcc gtgggcacgg
     3361 cagtccgcat cactatcgcc aagactgtgg ctatttt
//
LOCUS       DAWCYS010000182         3392 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1468_length_3392_cov_4.960144, whole genome shotgun sequence.
ACCESSION   DAWCYS010000182 DAWCYS010000000
VERSION     DAWCYS010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_3392_cov_4.960144"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          696 a         1121 c          844 g          731 t
ORIGIN      
        1 gccatccgct aacaactaac tgctagccac taacaacttt aaagggcttg tcgttgtgcg
       61 acaagccctt tttcatacaa ggaggatatc atgttccccc attggttcca attacgccat
      121 ttcctggccc tgttctttcc cctgctcctg acccagatgg cccaggtcgg gacatccgtc
      181 ttcagttcta tcttcagcgg tcaggccggg acgattgacc tggccggggt cgccgtagcc
      241 gtcaatatct ggtatcccgt ctttgccggc ctgtgcggca tcttcttcgg catctcgccg
      301 atcatcgccc agctccgggg cgctcagaag accgaggaaa tcccgacgta cattatgcag
      361 agcctctact tgagcctggg ctttacggcc gtcattttcc tggccggcgc ctttctgctg
      421 ccgccctttc tcgactttat gggcctcgac gcaccggtcc gctacatcgc ctgggaatac
      481 ctcaaggctc tggccctggg actggtcccc atctgcctgc aggcgacgat gcgctacatc
      541 gtcgacgccc acggcaagac ccacgtttcc atggccatct tagtgaccaa cctggtcctg
      601 acgatcatcc tcttccgcct gctcatcttc ggcgccggcc ccattccagc tatgggcggt
      661 atcggtacgg gctatgccat caccatcgcg tcgtcgctgt ccgtcctgct ctgcgggggc
      721 gtcctgcagt ggatggagcc tttccgctct taccatctct ggagccgcct gcggcctttc
      781 agctggtatc atggctatca tcagctggag ttgggcattc ccatcttcat cgccgtcttc
      841 tgcgaaacga gcctgttctc catcgtcggc ctgctcatgg ccgaattcgg caccttatac
      901 ctggcggcca accaggcagc catcagtttc tcgaccctga cctataccct gccctggagc
      961 atcagcctga cggcgactat cgtcatcggc tacgaagtcg gcgcccgcaa ctacgccgct
     1021 gcccggcagt atgccgtgct ctgccagatg acggccttca ccatcgccat cgccctcatc
     1081 gtcgtgactt accactttgt cgatcatatc gccgccatct tcacgagcga tgccgaaacg
     1141 ttctgggcca tccgcatgtt catcttctac gccgtcgcct tttccttctt cgatgccgcc
     1201 ggcacgccgg tccagggcat cctgcgcggc tataaggacg tcaggatcat cacttacgtc
     1261 gctttcgtga cctactggct catcagcatc cctatgggct atgccatggc ccacgtcacg
     1321 tcctacgggc cctatggcta ttggatcagc ctcatcatca gcctggccat caatgccagc
     1381 gccctgaact ggcgcttgtg gaagcatacg gcctggaaag tccctgtcct atcctcatct
     1441 actaaggagt gattttatgc caactaccaa acaattaacg gcttatgccc gcgtcctctt
     1501 gcagaagggc ctgaatctcc agaaaggtca gaccctggtc atcaatgccc ctgtcgaatc
     1561 gcacgacttc gtcgccctcc tgacgaaaga ggcctatacg gccggcgccg cccaggtcgt
     1621 ctgcaactgg cggtctgacg acctggcccg cctgcgctac gaacacgaag atttgcagta
     1681 tttcgagtcc ctgccggact ggcgccgcga tttcagcctt tactattacc ataagaaagc
     1741 ggccttcctc agcctcatct cggcgaatcc gtacctcatg gacagcatcg acacccataa
     1801 aatctttgcc cagcagaaag cccagaatga agccttgaag gaatacatcg acggcatgat
     1861 ggcctcgaag acgacgtggc tcgtcgccgc cgtccccagt gccgtctggg ccaaactgct
     1921 ctatccggac ctcgacgaag ccgccgctta cgaagccttg tggaagcaaa tcctccagtc
     1981 gtcccgtgcc gacggtgccg atgccctggc cgattgggac gcccatctgg ccgaattgaa
     2041 gaagcgccgg acctggatga cggaccagca tttcaccagc ctacactata cgaacggcct
     2101 gggaacggac ctgactatcg gcctgccggc cggtcatatc tggcagggcg gcatggaaga
     2161 aacggcggac cacctgctgt tcaatgccaa catcccgacg gaagaaatct attcggcccc
     2221 caaggcagat gcggtcaacg gcacggtcta cagcaccaag cccctggtct ataacggcaa
     2281 cctcatcgac aaattccacc tgaccttcaa agacggcaaa gtcatcgacg ctgccgccga
     2341 aacgggcgaa gacgtcctga agaaactcat cgccatcgac gacggcgcct gccgcctcgg
     2401 cgaagtagcc ctcatcccct atcattcgcc catttccctg tcgggtatcc tcttctatga
     2461 aacactcttc gacgaaaacg cctcgtgcca cctggccctg ggcgcatcgt acccgacctg
     2521 cctggttggc ggtgctgata tggacaaaga tgctgtcgcc gcagccggcc tcaacgactc
     2581 catgatccac gtcgacttca tggtcggctc gccggacctg accatcaccg ggaccagggc
     2641 cgacggcacg acggtgccgg tctttaccga tggcgactgg gcgcaataac gattttccat
     2701 tgacaatccc ggccatccgt gctattataa gacgcaacaa ccatataccc ttcatccaga
     2761 gcagctgagg gactggcccg atgacgctgc ggcaaccccc tatcgggaag gtgccaattc
     2821 caacggaaca ttccgacaga tgaaatgaaa ccgtatatac ggccgtcatc tgcatgacgg
     2881 cctttttttg tggttgcttt ctctagttac gcctattttg aggagttgat actgtgatta
     2941 cactgaaaaa tattaccaag acctacggcg gcgccgcaca ggtccaggcc ctgaaaggca
     3001 tcaacctgac catcgacgac ggcgagatct tcggtatcat cggcaagagc ggtgccggca
     3061 aatcgacgct ggtccgctgc atcaatatgt tagaaaaacc gacgagcggc caggtcatca
     3121 tcgacgacaa ggatttgacc aagatgaatg aatcccagct gcgggcagaa cgcaagaaca
     3181 tcggcatgat tttccagcac ttcaatctct tatcatcccg tacggttgcc gaaaacattg
     3241 ccttcccgct ggaactcgtc ggcgcttcca aagacgtcat cgaaaagaaa gtcgcttccc
     3301 tcctcgacct ggtcggcctc agcgaccggg ccggcaccta tacgacccag ctctcaggcg
     3361 gccagcaaca gctcgtcggc attgcccgtg cc
//
LOCUS       DAWCYS010000183         3382 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1474_length_3382_cov_4.481515, whole genome shotgun sequence.
ACCESSION   DAWCYS010000183 DAWCYS010000000
VERSION     DAWCYS010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_3382_cov_4.481515"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          729 a          913 c          978 g          762 t
ORIGIN      
        1 ctcccaacca cgacccacga gccccacgag ccacgagcca cgagccacga aaaaggcgct
       61 gtcgcgagac agcgccgttt tcatgccttt caagtatagg ttttttacta aaatatatgt
      121 tataatatga atataaggaa agattaactt tccgttttgt tatagttgcg aggaggaatt
      181 catatgaagc tcaaaaaatt gcactatact gtcgaggacg gtattgctgt cgttcagctc
      241 gattatccca agaatctcaa tgccatcgat gaagaaatgg ccgatgaact gctctacgtc
      301 gtccaggcgg ctgaagccga tgaatcggtg aaagtcgtcg tcttcaaagg ttctccccgt
      361 gctttttcgg ctggtggcga catcggctac ttctaccagc tcatcaaggc cggcggtgac
      421 gtcaatatgg acggcctcat cggcaaggta ggcaaggtca cggacggcct gaagaagatg
      481 agcaagatgg tcatcgcctc ggtcagcggt gccgcagccg gtgccggtgc cagcctggca
      541 ctgacggcag attttatcat ttgcagtgaa aacgccaagt tcatcatggc cttcgtcaac
      601 ctgggcctgg tccccgatac gggtggttcg tatttgctgg tcaaacagct ggggccgaaa
      661 cgggccatgg atatctgtgc gaccggccgg ccggtgagcg ctcaggaagc caaggacctg
      721 ggcatcgtct accgcatcgt gcccaacgag gacctcgacg gtgaaacgat gaagtttgcc
      781 aagaaattgg cgtcagggcc gatcatttcc tacaagaata tcaagcgcca gatctatgca
      841 gccgcctttg ccgaatacca gcactacctg gaaaatatcg aagtcccgtg ccagcgcgaa
      901 tgtgcccgga ccaaggattt cgccgaaggc gtctgcgcgt tcatggaaaa acggcgtccc
      961 cattttaccg gccagtaagg ctgctttgtg aagggagtga gtacggtgtt atcgaaagta
     1021 ttttctctcc aagatatcct ggaacatatc cacgatggcc agaccatcat gttcggcgac
     1081 tggcacggcc agtttgcagc cgatgagatc atcgacggca tgctggaaaa aggcgtcaag
     1141 gatatcaaag ctatcgccgt atcggcgggc tatcccggcc agggcgtagg caaactcatc
     1201 gtggcgcatc gcgtatcgtc catcattacg acccatatcg gcctcaaccc ggaagcactg
     1261 aaacagatgc tggcaggtga actggccgtc gaattcgttc cccaagggac ctgggccgaa
     1321 cgggtgcgct gcggcggtgc cggattgggc ggcgtcctca cgccgacggg cgtcgggacg
     1381 agtgtcgaag aagggaaaca gaagctggtc atcgacggga aagaatacct cctggaattg
     1441 ccgctccctg ccgacgtggc cctgtgcaaa gcgaccaaag ccgatacggc aggcaatctc
     1501 tatttccgca tgaattcacg ggcgacgaac agcaccattg cctatgctgc tgatttcgtc
     1561 gctgccgaag tcgaagaaat cgtccctgtc ggccagcttt cgccggaaga aatcgccatt
     1621 ccggcgcccg tcgtcgatat ggtctatgaa cggcagggcg aaaaacgcta tgtctgcccg
     1681 atgtggaaaa aggccagggc ccgtgccgaa gctaaagcgc gggaacggca ggaaagggga
     1741 tgaggtcatg gtcgaacgga aaggaagagc tttgattgcc tggcggtgtg cccaattctt
     1801 caaaaatggg gatttcgtca atttaggaat cggcctgccc ctgatgtgcg tcaactacct
     1861 gcctgaaggc gtagacctct ggctggaagc ggaaataggc acggtcggca gcggcccgtc
     1921 gccggattgg gaccatgtcg acattgactt catatacgcc ggctgccagc cggcctcagt
     1981 cattaccggc ggcagcgtct atgaccacga aacgtccttt gccttcatcc gcggcggtca
     2041 tatcgacgcg actgtcctgg gaacgctgca agtcgaccag gaagggaata tcgccaactg
     2101 gaccattcct gggaaattcg ctcccggtat gggcggggcc atggacctct gtgccggtgt
     2161 caagaaaatc atcgtcgcca cggaccattg cgaaaagagc ggccattcca agatactgaa
     2221 gaaatgtacg ctgcccctga cgggagccca ttgcgtcacc gatatcgtaa ccgaacgcgg
     2281 ctactttgaa gtcacaccgc agggcctggt cctgcgggaa ctggcaccgg gctataccgt
     2341 agaagatatc cgggcctgca cggaagcaga cttcatcgtc cccgaaacca tcgccgtcat
     2401 gggcggatga ggggaggtcg cacgaaggct tcgtaagcct ctcctactag gagaggtggc
     2461 ccaaatgggt cggagaggtt taaccttgta ggggatgttt ttatacatca cccgtcatgt
     2521 ataatgtggg catcattatt tcaggcgggc cgccctttgg gacggcccct acgaatactg
     2581 caaactcaat actcaaaaag aggctgtcgc acatgcgaca gcctcttttt cactattcct
     2641 tattattgcg gtcggcttgt tgtctggccg gctgctgggt ctgttgtttg gatttttctt
     2701 tggtcgtcgt tttttcggtc gtcgccgatt ttttcttcgg ttcttcccgg tcgtcgctgc
     2761 ggacgctgtt actggcagat gaagacgacg tcgtttcttt acggcgttcc atgcctgacg
     2821 gggcggagaa gtcgccaccc gattcatcgg acgtagccga cgacatgtaa tcattacaga
     2881 tttctgcagg gatcgtgcag ccgaagattt cgcctaagct gtgcgaaccc gtatcgtcgc
     2941 caatccatac ggctgtgacg atgtccggcg tgtagccgac gaaccaggcg tctttgttgt
     3001 cgtctgtcgt accggtcttg ccggcggccg gacggccgat ggcggcggct gtccctgtgc
     3061 cgtggtcgat gacgccttcg agcatagtcg tcatttcata ggcttcccgt tcggacatga
     3121 cgcgggtctt cgtttcttct ttttctaacg tcgatgcgtc ttccaggacg ttaccgtcgc
     3181 ggtcgaggat tttgacgata gccgtcggtt tgacgtggac gcccttattg gcaaaggtgc
     3241 cataagcact ggccatttcc agcggcgtga cgcctttggt caggccgccc agggccatgg
     3301 ccaggttgtc gtctttggcg tcgagggtgg taatgcccat cttcttggca ttggcgatga
     3361 tgttcttcgt gccgactttg tc
//
LOCUS       DAWCYS010000184         3359 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1480_length_3359_cov_5.546610, whole genome shotgun sequence.
ACCESSION   DAWCYS010000184 DAWCYS010000000
VERSION     DAWCYS010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_3359_cov_5.546610"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          717 a          962 c          946 g          734 t
ORIGIN      
        1 gaggaagacc ttgccagccc cgctgcggac ggctccatga gcggccatga gggcggctcc
       61 ggccatctgg ctgctgccgg tgacgatagc gatggtaccg tgagtccctt tatggctgtc
      121 cgggctgcgg ggcacgacga tttcagcgat atccgattcc tgcagcaaat aagccgggtc
      181 ttcttcgagc tgcatgagca gcggaccagg catgccgatg gggtcgactt caacgtggcc
      241 ggcacaagcc cggccgggat agaggaccag gccccgtttg agggcaccaa aggtgacggt
      301 catatcggca aagagcggac cgtcttcatc gtcgtcaccg ttgctgacag cccccgtatc
      361 ggagttgacg ccagtcggca tatcgacggc gatgaccgtg acggcaccgg cggcggcagc
      421 ggcgttgact tccgctacga tagaggcaat gggctggcgc aggctgccgt ggaagcccgt
      481 ccccaacatg gcgtcgatga cgacagaaga ggcgaggagc cgttcttgca ggacctgcca
      541 gtcagcttcc gactgcggcg ccggcaagag caggcacgtt tcatcgtcgg ccagctgctg
      601 cagcgtatgc aagtgctgac gcgcttcatc gctatagtcg gcttcttccc cgaggacata
      661 gacataggcc ctggccccgg cggctaagat atggcgggca atgacgaagc cgtcgccgcc
      721 gttattgcct ttgccgcaaa agaggacgac atccttgcca tcccagcctt ccagccaggc
      781 accggcccgt tcggcaacgg catggccggc attttccatg aggacagccg gcgcaatggc
      841 atatatgcca tgcatagcta tccgatccat attccgcatc tgagcggcag tagtaacttt
      901 ttgcatagta attctctcct atttttattg aaaagggcgc gtgtaatggg gcctgacggc
      961 cccgccatct caggccgcca tggactcaaa tgccgtgccg ctgcgcggcg tcaaactcaa
     1021 agggacgaca tcgcctcggg aaaccattgg ctcgtaaaat tgccccatcc ggggccaccg
     1081 taggggccgg cctgtaaagg cggcccgcta cgcgctgagg gcttcccgaa gcgtcgttgt
     1141 cagtttgagt ctgtcgcggc aacgccgcat ctgagtccga ggaaaggccg tggctcgtgg
     1201 ttcttggttc gtaaaatccc gccattagac aagaatgggc tgtggttaaa cctcacagcg
     1261 gcactatctg acagccaatc aacctctcag tcagcttcgc tgacagctcc cctatcaggg
     1321 gagccatggc ggactgccat ctgcggcctg cggactgcgt ttttttttac cctaacacgg
     1381 tgaaggcgat ggcgtaatcg ccgtcgtggg cgatggacag ggacggcagc tggccatcct
     1441 ggcccacggc ctgggcgtaa tagccatgga gctgataata cggggctccc aacgagtcat
     1501 ggcagacttc gacgtccgtc cattttccct ggcggaaacc ggtccccaag gccttgaaca
     1561 aagcctcttt ggcagcaaag atgccggcca gcgattcggc ccgccaggta ccatcgttcc
     1621 gctggcaata agccagttcc tgttccgtaa aaaagcggcc caaggaacgg ccgtgacggg
     1681 cggccatgtc cgctatacgg ctgatttgta cgatatcggt tccgcagtgc atcgcatttc
     1741 ccctcctttt tgggcctgac ggcccctacg catgcctctc aacctctcag tcagctttgc
     1801 tgacagctcc cctatcaggg gagccattgc ggccggccat ctgcggcctg cgaacggcgt
     1861 ttcccctcct tcaccacaaa aaggaggctg ccgcattggc gacaaccccc tctgtatttc
     1921 actaccactt ctgcattcgg atacaaacgc ccaatccttc caggatgacc cggaaagtca
     1981 taaaggatac ggtcatatcg tcatgaaggc cgtgggctac tttataggaa atggtgacgg
     2041 tctgatgttg atagtcgtaa cggaagccgt gcagattggt ctcaaaggtg tatttgggag
     2101 ccggaatggc aaactgcggc ggcttttcta cttccaggcc atgggtcagg cgttcgagga
     2161 tgacccgttt gggcagttca gactgcaaca tctcttctgt aaagctatcc aatacagaca
     2221 tgtatccccc tcagtcttcg tcgaagaagt gcgcgtggat agctttgacg gctttctcga
     2281 tgacatcttc tgcaatcagg caggtgatgc tgatttccga agtggaaatg atttcgatat
     2341 tgatgccgtt atcgccaagg acaccgaaca tgccggcagc gatgcccgga tgaccagcca
     2401 tgccagcacc gacgacagaa actttagcca tcttatcatc gatgaggata tcttcgacag
     2461 gcatttcctt gcgcagattg gcaaagacag ctttggcctg ttccaggtcc gtacgggcaa
     2521 tggtaaaggt aatatccgtt acgccttcgt tggcaatgcg gacactctgg acgatcatgt
     2581 cgacgtcgac gtgtttttct gccaattcag cgaaaacgcg ataagcgaag cccggtttat
     2641 tgggaatccc taaaacggtg atttttgcaa catttttatc cgaagcgacg ccgcggatga
     2701 gatactgttt gacttccata gagcaatcct cctgtatcat agttccttca tcttcagaaa
     2761 aagtcgagcg tacatggatg gggacgccga agcggctgcc catttcgacg gcacggggct
     2821 gcatgacacc ggcgccgagt ttcgccattt ccagcatttc gccgtatgta atctgttcga
     2881 ttttccttgc attgggaacg acacggggat cggacgtgta tacgccgtca acgtcggtat
     2941 agatttcaca aacatcagca tggatggcac cggcaatggc aacggctgtc gtatcggaac
     3001 cgccgcggcc cagcgtcgtg atatcgccat ggcccgtaat gccctggaaa ccagcgacga
     3061 cgacgatatt gccctgatcc agcgctttca tgatgcggcc cggtttcaat gccaggatac
     3121 ggcctttgtt aaaggaatcg ctggtcttga tgccggcctg agcaccggta aaggaaatag
     3181 ccttctggcc ggcttcctgg aaagccatag ccatgagggc gatggagacc tgttcgcccg
     3241 tcgtcaggag catatccatt tcacggccgt aagtcttaga ggtaatctcc tttgctaatg
     3301 tccctaaagc gtcggtagga tcccccattg cggaaccaac gattacgata ttgtcttca
//
LOCUS       DAWCYS010000185         3286 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1514_length_3286_cov_5.295884, whole genome shotgun sequence.
ACCESSION   DAWCYS010000185 DAWCYS010000000
VERSION     DAWCYS010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_3286_cov_5.295884"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          684 a          992 c          890 g          720 t
ORIGIN      
        1 cgtcacgccc ggcgtatcgg ccaggaggcg gacggcctgg cgaagcgtcg ccatacggtc
       61 acccagattg gcgccgatgc tgagatagaa cttagccatg gcgccgcctt tccaggacga
      121 cggcagcgtc ttcaaagggc ccgccgatgg gcgcctgagg cttgtggacg gtcacggtca
      181 gggcctggac cagcgggaac tgggccagga tgcggtcggc aatgacctgg cccacccgtt
      241 cgatgaggtc atacgacggc ccttcgacga cggccttgac gacgccatag acggcgctgt
      301 agtcgacggt ctgcgtcaag tcgtcggtct ggccggcccg ttccaggtcc gtttccagta
      361 ccaggtcgat gtgaaagggc tggccatggc gtttttcttc aggcagacag ccatgatagc
      421 catagaaatg aagtcccgtc aggacgattt tatccatctt tttcctcctc catgagcagt
      481 ttccaggcct ggtcataggc ggcggtccct tctttcaggc agcctgacga cgccgtcgtc
      541 acgatattcg agtcgatggc caggcggtcg cgcatggtca ggcacagatg ggtcgcctgg
      601 acgacgatca gcacgccttc cgggtcgagg ccgcggcgga tggactgaca cagttcgtca
      661 gtcatccgtt cctggagctg aggccgccga gccaggatat cgacggcgcg gacgaaatgg
      721 ccgaagccgg caatggtccc ctttttcggg taatacgcca catggacgga gccgaaaaag
      781 ggcagcaggt ggtgttcgca catggagtgg aagcggatgt tgcggacgac gaccaggcca
      841 tcggtctgag tcgcaatggg atcctgccac tcgtcatcgg gattttcctt gaggcctgag
      901 aaaaaatcag caaaagcctg ggccacgcgg tgcggcgtct tttccatccc cagggcctga
      961 aggtccagtc ccagggcctc taaaaactgc cgcatcgcag ccacggctct ttcattttct
     1021 tccaacgtaa ctagctcctc ctttatttat aaaatgaagt aatacgcagc gataaagata
     1081 aggccgccga cgatgatgaa aaagggactg gcatactgct gccagatact ctgtacgtga
     1141 tcgagataga tttcctgggg atccgccggg ttatagcgca aatccagttc gtcaccggcc
     1201 gtatagtccc attcgtcatc gccaaaggcg taacgggcct gataggtctg gccgtctttc
     1261 gtctggaaac ggacgacggg ataatacaag gtcccgctct tcatcttctt ttcttcaaac
     1321 cagagtagcg tccctacagc ggcgaccttg cagcgccgcg tcatgccgcg gaatttccag
     1381 ccgtgatagg tcccgacggc gatgaacagc acgccgagga tggccgggag ataataaaag
     1441 atctgcagta acataatacc ttcctttcta ttcaggacga gaagcggcga agcggctcac
     1501 ttccctgccg atgacagccc cgatgacgcc cgtcacgacc tgaggccagc tcatggcaaa
     1561 caagagcata tgccgcacag ccggcggaaa ggcgatgcag gcaaagagca ggtaaaaaga
     1621 gccgtaaagg atggccgcct tgcccagggc gccgccgcag aaagccggca ggccgtaccg
     1681 ctgccagcgc caggccgccc agacgtagaa actgttgccc aaggctacgg gaaagacaaa
     1741 gggaaaaaaa ggcagcatcc cttcgagcca ggcaaagacg ggcgtacagg cggcgacgac
     1801 caggccggcc cggcggccga cggtgaggac ggccacgatg aggcaggcat tgaccaggga
     1861 gccgacgacg aacatactga cctgcggcgg tatggggatg accaggcgca ggccctgcag
     1921 gatcaaggtc acggccagga tgagcccggt acgggtcaca ttttttatat cttctgtcat
     1981 aaaacctccc ctgtccaagc tggaaaaatt tgttataata ggatatgtca ttatcccaat
     2041 tatctttatt gtatctttcg aggtgtcaga tgtcaaaacg attcattcat tccatcaatt
     2101 atatgcgcgg tctctgcatg ctcggcgtca tcgccatcca cgtcggatcc gtcgctatcg
     2161 tcaacccgtc gcctaatctg ggactggtgg ccatcctgga aatcctgtcc cgcttttccg
     2221 taccggcctt tttcttcctg gcggccttcg gcatgttcta cagccagccc ctggaccagc
     2281 ccttcgtcta caaagactac ctgcggcgcc gcctgcggac ggtcttcgtc ccctacctgg
     2341 cctggtccct gttctacatg gtctacgtat cggtcatcag ccacaacttc agtgtcttcc
     2401 agccttacgc ctttttccgc accttatggt acggcctggc catgtatcac atttacttcc
     2461 tggtcatcct cttgtggttc tacttcctca tgccgttgtg gcggccgctc ctgcgcttca
     2521 tggacaaggc gccgtggctc tcttttacta tcctcttttt cgccaacatg gtcttcaact
     2581 tctattccag ctacatctgg acggtccatt ccgacaatcc cctcatccag gacgccttca
     2641 acttccgcct gaactacgtc gtcctgcact acctgttcat cttcatgttc ggtgccttta
     2701 cggcagaaca cttcacagcc gtgacggact ggctgaaacg ccacggcacg ctggtcaacg
     2761 tcttccaggc agcgacgaca gcgggtatgc tggccgccta ctacggcgtc atgcactacc
     2821 tgggctacga ccccttgtcg gcagtcttca ctatccatca gctcagccct atcggcatgg
     2881 cctataccct gtcgacgatg ctcttcctcc tctactggtg ggaatgccgg cctgtcccgc
     2941 aatgggtcca caaggtcttc tccctcctgg gcgactactc gtatcccatc tacctggtcc
     3001 atcccgtctt tctcagtgcc tgcacgggcc tggccgccca ctggcacatc tacctgcggg
     3061 ccgtccacat catcgccatc tacgtcatcg tcacagcctg tgccgcagcc tattcctggg
     3121 tcgtcgtcga acacagactg ccgaaatggc tcagcatctg cgtcaagggg aaatgacatc
     3181 gtataaaaaa ggcttgtcgc acgacgacaa gcctttttaa gttgttagtg gctagcagtt
     3241 agttgttagc ggatggcttt aaatttaaag gttttctctg aaaact
//
LOCUS       DAWCYS010000186         3282 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1517_length_3282_cov_5.353889, whole genome shotgun sequence.
ACCESSION   DAWCYS010000186 DAWCYS010000000
VERSION     DAWCYS010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_3282_cov_5.353889"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          915 c         1059 g          732 t
ORIGIN      
        1 tccctttcga tggcatcttt caatttctga ttcggctgaa gacgttaatc aatgagatca
       61 aaagtaaagg gcagctggct ctggtcgaaa tctagctgca gcggcgtcac cagattttcc
      121 gtactagcaa tggccaggtc cacatcggaa acgggtttat aatcgccgcg ggcccgcgac
      181 ccgaagagtt tcacccagtc aatccgcttg ccatagcggg ataaaatggc aaagatctgc
      241 cgccactgtc tgacactcaa tccgtacata gtactcctcc tttgggacgg ctcctgctta
      301 acctctccgg ccctgcgggc cacctcccct atgaagggag gcttgggcgt ccctatgatt
      361 ggcgggcggc cggcgacctg cgacctgcgg gccgcctttt aggcggcccc tacgattggc
      421 acttcgggca gtagtgggtg ccgcggccgt tcagtttggt gtattcgatg gtcgtgccgc
      481 agacggtgca gggcgtgcct tcgcggccgt agacgcggag gttttcctgg ttctgccctt
      541 ctttgccgtt gctgctgatg aagttgcgga ttgtcgtgcc gccgtgttcc aggccttcct
      601 ggaggacggc gacgatggct tcatgaaggc gggccgcttc tttttgggtc actgtatcgc
      661 tgtggcgcat gggccggatg cccgcccgga acagggcttc gtcgacgtag atgttgccca
      721 aaccggccac tttggtctgg tccaggagca gcgtcttcac cggccggtgg cagcccttga
      781 ggaggtcata gagccgctcc gtcgtgaagt ccgggttgat ggcgtccggt cccaggctat
      841 cgtagccctt gacgcctgtc ggcccgtctg acggcacgag ccacaggcag cccagggtac
      901 ggatgtcgcg gtagagaagg cgcccttcgc tgagcgtaaa aataagatga atggcccggc
      961 cagcatactg gccgggcttt tcttcataga gcaggcttcc tgtcatgcgc aggtgagcca
     1021 ggaaggacca gggaccgcgg cagcggacaa agaggtactt gccgcggcgt tccacgcctt
     1081 ccagggtctg gccggtcaag gcggctgcaa aatggtctgc cgtcgtattc ttgatgagcc
     1141 tgggcaggtc caggtcaatg gccgtaatgg tcttgccccg gacgtgctcg tcgaggaagg
     1201 cccgtatcgt ttctacttcc ggtaattccg gcatagtctc acctacttcg cttcttccca
     1261 gttgacgccg ctgtggatgt cgacgacgag gggcacggat aaatcgacga tgtgttccat
     1321 cgtatccttg aggagctgtt ccacttcatc cttttccccg gccgggactt ccaggaccag
     1381 ttcgtcatgg acctggacca ggagctggct cttcaaatgg cgtttttcca attcgtcctg
     1441 gacagcgttc atggccagct tgatgatatc ggcggcgctg ccctggatag gcgtgttcat
     1501 ggccgtccgt tcggcaaagg accggcgcgt gaagttcttg ctgttgattt ccggcaacgc
     1561 ccggcggcgg ccgaacatgg tgacggccat gcccgattcc ttcgccgagg cgatgaggcc
     1621 gttcatgtag tcgtggaccg tgttgtagcg ggcaaaatac gaatcgatgt attccttggc
     1681 tttttggcgg gaaatggaga tattccgggc caggccgaag tcgctgatgc catagatgat
     1741 gccgaagttg acggccttgg catgggaccg ttcttccggc gtgacctcgt ccagggggat
     1801 gccgaagact tccgacgccg tccggcggtg gatgtcctgg cccttcttga agccgtcgat
     1861 catagtcggg tcctgggaca agtgagccag gaggcgcaat tcgatctgcg aatagtcgcc
     1921 agagacgatg tagtcatagc cttcgccggg cacgaagagg gagcgtatct tgcggccctg
     1981 ttcggtccgg atggggatat tctgcagatt cgggtcggaa ctgctcagac gtcccgtcga
     2041 cgtgaccatc tggttgaaat gggtatagat gcgccccgtt tccggcacga tgagcggttc
     2101 caggccatcg agataagtgg aaatgagttt cttcaaggcc cggaagtgca gaatcttgtc
     2161 gatcatggga tgcttgtcgc gcagggcgtc gaggacggaa ctgtccgtcg aatagcccgt
     2221 cttggtcttc ttgatgaccg gcagctgcaa cttctcgaag aggatgacgc cgagctgttt
     2281 cggcgaattg atgttgaagg tctcgccggc gtcgtcgtaa atggcttctt ccagggcatc
     2341 agcctggcgg gacaattcgc tcttcatggt catgaggcgg tccgtatcga cagtgaagcc
     2401 gttcatttcc atgacggcca gcgtatggat gagcggttct tcgatggtat cgtagagctg
     2461 tgtcagggat gccttatcca attcagctgc cgctttgggc cagacctgga gggcaaaagc
     2521 ggcatcgcag gccagcttct gtccgtcgtc ttctgtgcct tcgtccaggc tgacgtcgaa
     2581 ggtttcggaa atatacgtca ggccatagga cgtccgcgtc gggtcgagga gataggctgc
     2641 caggatgatg tccttcaagg gcaggcggcc cggagcgacg cgggatacga ccttggtcag
     2701 gtccttgccg tcatcggtga cgacggctgc cgcttcggtc aggacggcga gataggcgtc
     2761 gtaatcgctt tcgggaacga cgaaggcgcc atcatcgacg gccatggcca gggccgtcac
     2821 agccgggatg ggctggctgc cgtcatagcg ggcggcgacg gcgacggtct tccctttcag
     2881 gctggccgcg cccggtgcgt ccatgacctg gacggccgct gccggtgccg cggcgtctgc
     2941 cagatgagag aagcggtcga aggcggcaaa gcgttccaga agattgcgga agcccaggct
     3001 gcggaagagc tgggccacgt cttcctggcg cggctggggc tggaacatgt ccatggtgta
     3061 atcgaggggc acgtcacagc ggatagtggc caggtccttg gacaagacgg ccttgtcctt
     3121 gttgtcggcc agtttttcct gcagcttctt gcccttcact tcgtccaggt gttcgtagac
     3181 gccgtggacg ctgccgaact ggctgaggcg ctagagcgcc gtcggatcgc cgccgcccgc
     3241 gacgccgggg atattatctg ccgtatcgcc tatgagcgcc gt
//
LOCUS       DAWCYS010000187         3279 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1520_length_3279_cov_4.075993, whole genome shotgun sequence.
ACCESSION   DAWCYS010000187 DAWCYS010000000
VERSION     DAWCYS010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_3279_cov_4.075993"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          768 a          834 c          828 g          849 t
ORIGIN      
        1 cggcggcacg gcggcataag tcgtatgcat aggcatccct ccaaacgtga acgtccccat
       61 catcacagcg gccatcagcc aagcggctct tttcttgctc ttgaatgtct tcatggaaaa
      121 aaccccttcc ttattgttgc atcgttgcag ctcaccgctg catacccagt tgttttactt
      181 gatgcattca ttataagaga aggagtatat aattacaaat acttggtttt aatagtctat
      241 gatacacttt aggcatgatg agagaacgcc tcgtccaggg ccgccacgac gagacgaccg
      301 gcgcgggaga agacctggtc tctgcgccag accacataat tcgccgagta aatcacgggg
      361 tccaaaggac ggaatacgag cccgcgggac gaacctacgg catccgccat gcccttgaag
      421 caaagcgcac tgcccatgcc cgcctcaatg aagaagattg tattgtagag caagttgtag
      481 gtgccggcca cctcgatttt ctccgctgcg ctgccgaaat aatgatccag ctcgtgatgc
      541 ttcattgcct gatcataaaa aataatagga tcctggaaga gatcgccgcg ctgtacggcc
      601 tccatgcccg cccgagcctg gtccgcccgc atcatgagac cccaggcatc gtcgtgcggg
      661 atgcggcgaa acacatacgt ccgcggtgca tccgcccggc agacgagccc gaagtcgagc
      721 aatcctttct gcagagaatc gtagatcagg tcttcattgc cgctgaccag atggaactgg
      781 atgtgcggat aggagcggcg cagcggcgcc aggactttcg cgacacaggc catcgcctgc
      841 gtctcgccgg caccaatgta gatgtccccc gccacgagct catccatctt ggcaaactgc
      901 tccttcgtct tttctgtcag gctgagaatc tcctgcgctc tcgccttgaa tatcagtccg
      961 gcttccgtca gctgcgtctt gcgcttcccg cgcacgagca gctgcacgcc cagttcgtcc
     1021 tcgagatccg acagctgccg cgaaagcgtc ggctgcgtca ggtgaagttc ctccgccgcc
     1081 ttggtgatgt tctcatgcct agctaccgcc ataaagtact gcagtacgcg tatctccatt
     1141 ctcccactcc tccatcatct tctatgattg gtacacctcc tctattatat acgcaaagcc
     1201 gcaatataga aaagaagtca actaccccca cctaacgcct tcgacgtttg aggtgggagc
     1261 ttgtaagtcg gaactacata ggtactaacg acatctaaaa gatgtcttcc taaatccgcc
     1321 tacatcatcg ggtggctgac aacacccgct ttgctaagga gtttactccg aagcagttgc
     1381 tattctttcg taacgaggat ggttatctcc ggaaatccac atagttccga ggagttggat
     1441 attcatagct ccgatacggt catcgttaga tctgtaacca cactggcaac gatataagtg
     1501 tttgtgatga tcacgatttt ctttgtggat agtgccgcat ttgggacaac gttgagaggt
     1561 atacttagca gataccttca ggacttcgga acgattttca tgagccttat aggtcaggaa
     1621 ttgctccaac tgatagaacg cccagctgcg caggtcgtaa ttttgtttag cagtttttga
     1681 aagattggac gcttcgaagc taataccggt cagatcctca agcacaaaca gcgtatcttt
     1741 gccgtatttt tcaacgagtg tcttagaaat ctgatggttt acatcagaca tccaacggtt
     1801 ctctcgtcca gaaatggctt tgagtcgacg ctttgctgat tttgtaccct tagagtggag
     1861 ctggcggcgg aattcctgga atttatggcg tttagtagca atcttcttac cagagacgaa
     1921 ttcagttttg ccctgttcgt cgtaactaac agtaagaaaa cgtaatccgc ggtcaatacc
     1981 gacaacatga cggacgtttt ctttctgaaa atcttcgaca gcttgggtta cgggaatgtg
     2041 gagataccat aaacctttga gttcgaccag ttttgctgtt ccaaggtcat aagcgccatc
     2101 aaggtattca gcgaaatgtt cgccctcaaa agtgcatttg gttctttttc cgagtgtatt
     2161 gatagatagg atttgaccat catcaacgaa actgtaatct cggttacgga ccaaatcagc
     2221 ttgcggacga gtaaagaata tcggcttcca aagccattcc catgtcttgg cgatgcgttg
     2281 ccagttgcca ttctcatcct tatatctgta gggattttgg aagagctgtt gttttaccgt
     2341 tttatatttg gcaatcgtcg tcttgataga agactgggcc agctgcgatt tcagaccaaa
     2401 ctggctgcgc aggtcactgt acagttctct gttgagactt tggtaggcca tgttaaatcg
     2461 gttgtcgaaa atgtactgcg acacgaagtt acaagcctga cggtattgtt ccgtcatctg
     2521 acggaacagc gtctcttgtt cgggagtaac gtgtatacga agttttattg ttttagttag
     2581 gttagacatt ttttcacctg cctttctaaa atctatacta cttcattaaa attattatat
     2641 cataattttt ttgctaaaag tctattattt attattgaaa ggaggggagg tgcggctcct
     2701 ctcatgacta aagtcacgag tctccgccgc accatgaaga tgaaattcaa atacagctat
     2761 cgcagatagg tctggaactg ctggcgattg gcctggatgc ggggattggc ggagttgatc
     2821 tggccggccc gttccagcca ggatccggct ttgtctgcgt tccccttacg gaggtagagg
     2881 acggccatgg cgttggccgc atctgccgac tggggcgaaa agcgcaggat ttcctggaag
     2941 gactgccggg ccttatcgtc ctgcttcaat tccatggcca cgaggccgcg gttataccag
     3001 gcctgcaaat attcgggacg gcaggcgacg gcctggtcgt aactagcgag ggaagcttcg
     3061 agctcccctc ttttttgttg cgtcaaagcc aggtcatacc aggcagcagg cgacgtcttg
     3121 tccaaagcca gggcacggcg gaagtcccgt tcggccagcc agtaatcgtg gcggtcgaag
     3181 aagaggatac cccggtagag ataggcgtgg gcgtcgtcgc gaggggggag gcgcatctgg
     3241 actcccgggt ccacgttatg gtgggttacg tcaggtgtg
//
LOCUS       DAWCYS010000188         3261 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1527_length_3261_cov_7.128509, whole genome shotgun sequence.
ACCESSION   DAWCYS010000188 DAWCYS010000000
VERSION     DAWCYS010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1527_length_3261_cov_7.128509"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          781 a          805 c          896 g          779 t
ORIGIN      
        1 ttcaaagtgt ggttacgcga tatttatttg ttcaaaaagt tgtaaactgc tgaattgaat
       61 tatattaagg atacataagg aaggtggttc ggtgagtcgt atggatcggg attttgtggt
      121 atcacaattt acgcggtacg tccagggctt tgaccaggag gaagaaggcg tccggctgaa
      181 gtatgctcat tctttgcggg tgagcgggtt gtgtgaacag attgctttga gtctggattt
      241 gtttcaggat gatgtggatt tggcctggct catcggcgtc ctccacgata tcggccgctt
      301 tgagcagctg cgggagtatc atacgttcgt cgattatcgg tccatggacc atgccaaata
      361 cggggcccat tacttgtttg ctgacggtca tatccgcgat tttctggacg atatgagtca
      421 ggataatgtg atttgtatgg ctattgacca gcataatgtc tatcagcttc gccgggattt
      481 gacgccgcgg cagcagctct tttgccagct catccgcgat gccgacaaaa tcgatatttt
      541 ccgggtttat gtcatgtata tgcgccaaaa gaagaatatc tggaacgtcg actggtctga
      601 ctttgaaaac cagcctattt ccgatgtcgt catggctcag gcccgccagg gaaaactggt
      661 gcggacgcag gataagaaga ccttcatgga cttttatgtc ggtgccctct gcctctattt
      721 tgatttagtc tatccccgga gccagcagct ggcgcgggaa cagggctatc tcgataggct
      781 cctcgatttc cattcgcaga acgccgatag tgaaggtaaa ttggaagaaa tccgtcgttt
      841 gattcgcagg acgggaacat tgcctcagcc ttcatttatt gatacaatat ataaaaatat
      901 gtagtatata tttgattgat gacaaaatga ttattgtatc cttagtagta gggggcttaa
      961 aattatggaa atattgatta ctggcggtgc tgggttcatt gggtcacaca ttttggcaca
     1021 gctccaggga cggcgcgata tggacgtcgt cgtgtttgac aatttgtcca gcggatccaa
     1081 agaacatgta ccggctggga tggaactggt agaaggcgac gtctgcgatg aagcggctgt
     1141 agatgccctt tttgccgacc atcactttga tgccgtcatc catttggcgg cccagacgat
     1201 ggtcccgact tcagtggaac agcctgtcct cgattgccag attaatctgg aaggcgtcct
     1261 ccacgtgctg gaagcctgcc ggatccatgg cacagggcat attctctttt cgtccagtgc
     1321 cgctgtctac ggcgacaacc tccatatccc cctgaaggaa acggaacggc tcgtgccgac
     1381 gtccccttat ggtatcacta agatggcgac ggaacattac atgcgggtct atcacgaatt
     1441 atctggcatg gaggcgacgg tcttccgctt tgccaacgtc tatggcgaac ggcagggcga
     1501 aaagggcgaa ggcggcgtcg tcagtatttt ctgcaagctt ttgtcccagc gtcagggaat
     1561 cacggttttc ggcgacggca atcagacccg cgatttcgtc tatgccggcg atattgccca
     1621 ggccatcatc cgggcgctgc cgctcaaagg ctgtcacacg atgaacgtgt cgaccggaca
     1681 ggaaacgagc atcaacgatt tgatccgcag ttttgaaaaa gccgtcggct atacggtccc
     1741 cgtccagtat acggcaccca ggacgggcga tattctgcgg tcggtcctca gcaatgaggc
     1801 cttgaaaagg gatttaggtt ttgtgccggc aatggatttg gaagaaggaa tccgccggac
     1861 ctatgactgg tatcgcagcc aatggacgaa gtaaggcgct ttttgccttg acaggcttcc
     1921 caaaacatga cacaatgaat tagatatgcc tatatacatt tatatacagg aggggtagcc
     1981 tatggcagat gatcgcatca ttacggcttt ggacgtccat tcactggatg atatgaagaa
     2041 gctcgttgaa acccttggcg atagtgtttc tttttataaa gtcgggatgg aactctttta
     2101 cagtgccggt cccgatgcag tacggtattt gaaggaccag gggaaacatg ttttcctcga
     2161 tctgaaggtc cacgatattc ccaatacggt ggcccagagc atccgcgccc tgacccgcct
     2221 cggcgccgac ctcatgacgc tgcacggcac aggcggccgg gctatgatgg aagcggctgc
     2281 agaagctgtc cgcgatgaag cggccaaact gaatatcgaa cggccgcgcc tgctggctgt
     2341 aacggtactg accagtatcg acgaagacgc ctggaaggaa atcggcggca agtacagcat
     2401 tgccgaatcg gtcaagaatc tggcgaaact ggccaaggaa gccggtatcg acggcaccgt
     2461 ctcttcgccg tatgaagcta aagaaatccg ggaaatgaat ggtcccgatt tcctcatcgt
     2521 cacgccgggc atccgtccga cgtttgccgt agccaatgac cagaagcggt tcacgacgcc
     2581 gtctcaggct ttgcgcgacg gggcatctca tttagtcatc ggccgtccga ttaccaaggc
     2641 cgccgatcct aaggaagcag cccaaaagat tttagcagaa attcaggggg tataatcaat
     2701 catgatgaca gaagcagaag taagacagtt gttagtagac accaaggccg tcctcgaagg
     2761 ccatttcctc ttgacgtcgg gcctgcacag cccgctgtac gttgaaaagt tcaacgtcct
     2821 ccagcatccg gaatatacgg aaaaactctg caaggaattg gcagaacgtt tcaaggacca
     2881 gaacgtccag accgtcatgg gtcctatgac cggcggcatc ctgctggctc atgaagtcgg
     2941 caaggcactc ggtacgcggg ctatcttcac ggaacgtgaa aagggcgtta tgacactgcg
     3001 ccgcggtttc cacatcgaac cgggtgaacg cgtcctcatc gtcgaagata tcgtcacgac
     3061 tggcggttcc gtccaggaag tcgtcaatgt cgtcaagaaa gccggcggcg tcatcgtcgg
     3121 tgtcggcctc ctcgtcgacc gcagcggcgg taaagctgaa ttcggcgtac cgaaagataa
     3181 agtccaggct ctcctgcatt tgaccgtccc gacgtacaaa ccggaagaat gcctgctctg
     3241 caaacagggc atccccatga c
//
LOCUS       DAWCYS010000189         3258 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1528_length_3258_cov_5.722448, whole genome shotgun sequence.
ACCESSION   DAWCYS010000189 DAWCYS010000000
VERSION     DAWCYS010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_3258_cov_5.722448"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          771 a          886 c          874 g          727 t
ORIGIN      
        1 ttttccacgc gccgcgggtc cctcgaggcg atgtcggcat gctccgtgtg ttgccgcgtc
       61 gcggcatctg agtccgaggc gaactgcgaa ctgcggcctg cggccggcgt cttatgtccc
      121 ccttaacaat cttatcgcta atgtggtata ataccctatt acggataact ttatctcatt
      181 agcgattttt ttgaaggagg attttttgga tgcggccttt tgttcatttg catgtccata
      241 ctcaatacag tttgttcgac ggcttgtgca agattcccga tatggtgaag cgtgccaagg
      301 agctgaatat gccggctgtg gcgattacgg atcatggcaa tatgtacggt gtcatttatc
      361 tgtataagga agccgtagcc caaggcatca agccgattct ggggtgtgaa gtgtatatga
      421 cccggggcag ccgttttgaa aagaagacga aagaacggct ctgtcacttg attttgctgg
      481 ccaaagacaa taagggttac cacaacctca tcaaaatcgt gtccaaaggt tttgtcgatg
      541 gggaaaataa ttaccataag ccccgcgtcg actacgattt gctcgaacag tatcatgaag
      601 gcctcatcgc catgagtgcc tgcatcgaag gccacatcca gcaggatatc ctcaatcaca
      661 atgaagaagg ggccaagcgg actttggacc ggctggtttc gatttttggc aaggatgatt
      721 tttacttgga agtccagaac catgggattc cggaagaaaa agttgtccgc acggtattca
      781 agcgctggtc ccaggaatat ggcctaaagc tggtggctac caacgattat cattacatcg
      841 accccagcga tgcagctgcc caggaggtca agctctgtat ttctacgggc agtactctcg
      901 atgacccgtc gcatttccgt ttttataata atgaatttta tatgaagagc ggtgacgaaa
      961 tggccgacct ctttgccgac atgcccgaag ccctggatac gacgctggaa atcgccgata
     1021 agtgcaacgt cgagatttct ttcagtgagc gccatctgcc gcagttcccc gtacccaagg
     1081 gggaaacgga ttcgtcctat ctgcgcaagc tctgcgagca ggccctgccg gaaaaatatg
     1141 accccattac gccggaagtc cgcaaacggc tggactatga attgggtgtc atcgacacta
     1201 tgggattccc gtcgtacttc ctcatcgtct gggactatgt caaatacgcc agggaccatg
     1261 aaatccccgt cgggccgggc cgcggctcgg cagcgggctc ggtcgtcgcg tatttgctgg
     1321 gcattaccgg tctggacccg ctcaagtacg acctcctctt tgaacgattc ctcaacccgg
     1381 aacgcgtcac tatgccggat atcgacatgg acttctgtta tgaaaaccgg ggccgcgtca
     1441 tcgattacgt tacccggaaa tacggtgtcg accacgtctc gcagatcatc actttcggga
     1501 ccttggcggc caaagccgtc ctgcgtgatg tcggccgcgt cctcgacatc cccttgagtg
     1561 aagtgaaccg catcgtcaag atggtgccta acgaattggg catgactctc gacaaggccc
     1621 tgaagatgag caaggaattc cgcgaggaat atgaacagaa tgcgacggtc caccgcctcg
     1681 tcgatttcgg taaatccctg gaaggcctgg tccgccattc atcgactcac gcagcgggcg
     1741 tcgtcatctc ggcggctcct gtcgacgact acgtgccggt ccaacattcc aaggaaggcg
     1801 atttgacgac acagtacgaa aaggacctgg tcgaagaact gggcctgctc aaaatggact
     1861 tcctgggctt gcggacgctg accgtcatcg gtgatgccgt caagatgatt aaaaaggacc
     1921 acggcatcga cctggacatc aatgccattc ccctggatga cccggaaacg tgccggctcc
     1981 tgaccgaagg cgatacggcc ggcgtcttcc agatggaatc atcgggcatt accaacctgg
     2041 tcaaggaact ggcgccgagg cactttgaag acatgatccc gctcgtcgcc ctctaccgtc
     2101 cggggccttt gggcagcggc atggtcgatg atttcatcaa gggcagtcac ggtgaaaaga
     2161 aagtcaccta cctgcacccg ctgctggaac cgatcctcaa ggatacatat ggcgtcatcc
     2221 tctaccagga acaggtcatg cagatcgcct cggtcatggg cggcttttcc ttgggacaag
     2281 ctgatttgct gcgccgcgcc atgggcaaga aaaaggaatc catcctggtc gcccagcggg
     2341 ctaatttcct ggaagggacg cgaaagaacg ccattcccga cgacattgcc aacaaggtat
     2401 tcgacctcat ggtctatttc gccggctatg gtttcaacaa gtcccattcg gcagcttatg
     2461 cctacgtagc ctggcagacg gcgtacctca aggcccatta tcggccggaa ttcatggcag
     2521 cgactatgac cagcatgatc aacgatgtca gtaaaatcag ttactacgtc gcagagtgcc
     2581 gccgtcacgg cgtccaggtc ctgtcgcctg acgtcaatgc cagcatatcc atgttctccg
     2641 tccaggacgg ggccatccgc tttggcctgt ccggcgtcaa gagcgttggc gccaacgcca
     2701 tcgaagccat cgtcaaggcc cgcgaagagg gcggaccatt cacatcgctc ggcgatttct
     2761 gcagccgcgt cgattaccat atcgtcaaca agcgggccct ggaaagcctc atcaaatgcg
     2821 gggctatgga ttcttttggc aagaagcggt cccaactcat ggccgtcatc gaccaggcca
     2881 tcgccatggg gtccctcagt cagaaggact acgcgtcggg tcagatgggg ctcttcgacg
     2941 atgccgaaga tgtcatgacg gaactggaat atccggatat cgaggaatat cccatcgaaa
     3001 tgcgactggc catggaaaag gaatacgatg gtttttattt cagtggccat cccctcaata
     3061 aatataagga cattatgaag aaattgacgc ccttgtcggt cctctatggg gaagacgggc
     3121 ggcagtacga tggcaagatg atgcgcatcg gcggcctgat tacggccaag aagcaggtca
     3181 tgaccaagcg aaatgaaaaa atggccaaca tcaccttcga agacttcaac catgacgtat
     3241 cggtcgtctt cttcccta
//
LOCUS       DAWCYS010000190         3256 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1530_length_3256_cov_12.248672, whole genome shotgun sequence.
ACCESSION   DAWCYS010000190 DAWCYS010000000
VERSION     DAWCYS010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_3256_cov_12.248672"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          666 a          880 c          951 g          759 t
ORIGIN      
        1 caaggctccc tttatagggg agctggccgc caaaggcggt ctgagaggtt aaagggcagc
       61 ccctacgcac aaggccgctg gccgtgtttt tttgcaatct atgacctctt tctcccccat
      121 cgcctgtatg cgatcccgta ggggacgccc agagggcgtc ccgctacgcg ctaagggctt
      181 cccgaagcgt cgttgtcagt ttgagtctgt cgcggcgaag ccgcatctga gtccgaggcg
      241 gcctgcggcc tgcgaactgc gacctgcttt ttccttcatg ctataataag gttaccatgc
      301 tgagagagga gtcatcatca tgaatgaaaa gattgagcaa tacgtacgga atcacggccg
      361 tgattttatg gaatggcttt gtgatattat ttctattcct tcaccgaccg gacatgaaca
      421 ggccaagggc gaatggatat tgaatctttt gcatcaatgg ggcgccgccg gggcgtatcg
      481 tgatgccgca ggaaatgtgg tctatccctg ccatgtaaaa agcggtgaaa aggtcgccct
      541 ttacaccgct catatagata cggtattcca ggatttgcag gaaatccaca tccgccagac
      601 aggtcacatt ttgtcagcgc cgtcgtgcag cgacaacagc gccagtatcg ccggtctatt
      661 atttatcatc aaaatgttcc acgatttaca gctcacgccg ccgcaaggtc tgctctttgc
      721 cttcgacgtc ggcgaagagg gcttgggcaa tctcaaggga atgcggcagg tcatggccga
      781 ttggcacggc cgcattgccg aagtggtcgc cgtcgattgt actttcgata cttttgtgac
      841 gacagctgtc ggctcccgcc gctatgccgt caccgtcgaa gccgccggcg gccacagctg
      901 gatgcatttc ggccagtcca atgccattgc cgaagcggcc cgcatcatca gccagctcta
      961 tgcactgtcc gtgccatccc agccaaagac gacgtacaat gtcggcacca tcacgggcgg
     1021 cacgacggtc aattccattg cggcaaaggc cgtgttcact gtcgacttgc gctcggaaaa
     1081 tgctgatgaa ctggacaagc tggaccaaac ttttcaaagc ctcctggccc atactgaagg
     1141 ggaaacggtc cacatcaaaa cccagctgct cggcgaacgg ccctgcagtc gcctggacac
     1201 caatcccctg gcagaccgca tcacggccat ccggcggcgc cacggcctgc cgacgccgtg
     1261 cattgccggc agcaccgatg ccaacattcc catgggccag ggtatcccgg ccatttcctt
     1321 cggtttctgc cgcagccacg gggagcatac ccttcaggaa tcgctggacg tcgatacctt
     1381 tgtgcccggc atgatccaga tgttggaatt catgggagtg taaaaaaaag gcttgtcgca
     1441 tatgcgacaa gcccttttat cgtaggggcc gtcccaaagg gcggcccgtt gtggctagtt
     1501 gttagtggtt agcaaatggt aaaaaattta gcgaggccta ccgtgcgttg gcttcgccac
     1561 ggaagatggt gccacggctg gcgtctacgg tgacgacttc gccgttttcc agggtatcgg
     1621 cagcatgggc agcgccgacg acggtcggga tgccgagggt gatggcgacg atagccgatt
     1681 cgctggtgag gccggcttct tcggagacga tggcgctggc ccgtttcatg tagtcgacga
     1741 aatcagcgcg ggccgaacgg actacgagga tgcagccgtc ggtgaagttc ttttccaggt
     1801 cttctttcga ttcggcgatg cagactttgc cgatagccga gttacggccg ataccggtac
     1861 cgcggaggag gacgtcaccg acaacctgga cgcggatcat attggtcgaa ccggctttgg
     1921 caacggggac acctgccgtg aggacgacga ggtcgccggc tttgatgagg tcgttgtcga
     1981 gagctgcaaa gatagcattg tcgacgacag catcggtgtc gctggcttct ttaccggtga
     2041 cagcctggac gccccagttg agctgcaggc ggcggacgat ggcttcatgc ggcgatacgg
     2101 ctacaatcgg gcaggccgga cggaatttag aaatcatctg agctgtgccg ccgcgttcag
     2161 tcgaagcgat gatggctgca gcgtgaaggt cttgggcaat cttgacgacg gctttgccga
     2221 tggattccgt cgtcgtagcc gattcagcgt cgacgtcttc ataggacttc ggcgtgaagt
     2281 tgctttccgt aaaggttgcg acgcgggcca tggtttctac cgcttccacc ggatagagac
     2341 cgccagccgt ttcgccactg agcatgatgg catcggtacc gtcgaggatg gcgttggcga
     2401 cgtcgctcgt ttcagcacgg gtcggacgcg ggttgttgca catagattcc agcatctgcg
     2461 tagccgtaat gaccggttta cctgcggcct ggcatttatg gatgaggact ttctggagca
     2521 tcggcacttc ttctgccggg acttcgacgc cgaggtcacc gcgggcgacc atgatgccgt
     2581 cgctcatctt gatgatgtcg tcaatgttgt tgaccgcagc ctgacattcg attttggaaa
     2641 tgatcttcat atggctgtcg tgtttttcca aaatcttgcg gatcgtcagg acatctttgc
     2701 cgcgctggat gaaggaagcg gcaatccagt ccatgttcat gcggcagccg aattcgatat
     2761 cgtcggcgtc tgtatcggaa acagccggca ggttgatggc aatgcccggt acggcaacgc
     2821 gtttacggtc gctcatcttg ccgctgttga ggattttcgt atggatgtct tggccttcga
     2881 cgctgacaac ttccaagttg accaggccgt ccgacaagag gatgtggtcg ccggctttga
     2941 cgtcctgcgg taattcttta tagttcatgg aggcaatagt ttcatcgcct tctacgtcac
     3001 gggccgtcaa ggtaaagtct ttgccggctt ccatcatgat cgaaccattt ttaaatttgc
     3061 ccaaacggat ttccgggcct ttcgtgtcga ggagcagtgc aaccgggata cccagtttct
     3121 gagagacagc gcggaccatg tcgatgcgct gtttatgttc ttcatgagaa ccatgggaga
     3181 aattaaagcg ggcaacgttc atgccggctt taatcatctt ttcgataatt tccggggtat
     3241 tggaactggg gcctaa
//
LOCUS       DAWCYS010000191         3253 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1533_length_3253_cov_5.525954, whole genome shotgun sequence.
ACCESSION   DAWCYS010000191 DAWCYS010000000
VERSION     DAWCYS010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1533_length_3253_cov_5.525954"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          574 a          885 c          894 g          900 t
ORIGIN      
        1 cttgagtgca gtctgctctt caattttcag ccagtcgtcg aggaaaggct tgaggtcttc
       61 cgcctgctgg cccttcgtga tgaggtccgc taaatatcgc ttctgggccg cttcatcacc
      121 gctccgcatg gtatcgagta aggtctttag tttactgtcc attcatgggg cctccttgct
      181 ggggtacttg cggggcttgc ggttccggct gtgccggtgg ggcttgcggc gtcatctgtg
      241 cccggtgggg ccgtgcgagc ttctcgatca tgggctgtgg gctggtgttg cctgccgtca
      301 gggtgttgat gatattcacc tgtgcgtcaa gcggcaaatc gttcatgttc gcccggatag
      361 acgggatgga cgccacggcg gccttgcctt cgtagtcggc ctgcttcaaa gtgagctgtt
      421 tctgcaaatc catggcttcc tgcgcttgtg cagtctgtac agcctgctgg gcttgctgtg
      481 cctgcatctg ctgggcttcc tgcgaatccg ggtccagcaa aatgccctgc gtattcttga
      541 ggcccatttc ttccaggagt gccgttccgg cagcgtaata gctcttggga gtcgctacgc
      601 ctgcctgtga taataccggg tatacgttgc taaggagcat catatagctc tgtatccgtg
      661 cttccttcgt ccctgcgccg ttaccgacgt tgataatgag gtcgtagtcg atgtcaaggt
      721 cttcgctctt gacggagact tcctcgtctt taaaacggaa ggtctgcacc ggttcgccgt
      781 acttcttgtt gagcaggata aggaagcgaa ccatgggtac aatccagttt tctgcgaaca
      841 acctggcaat caaccgtatg cgcttatctg actggccgag aatggccgtg atacccgtgg
      901 ccgtactgtt gagagagttg gcgtctaagc cctggttgta cttcgtactg ccggtacgat
      961 tttccagctc gctttctgcg tagttgacaa ggtccatcgt gagcggtgag atattcgccg
     1021 gtggcgggtt cgcaatagcc gcgttcgggt cgcccttaat cgggacgtac tcgtcgccgt
     1081 tacgcagggc gtccatatcc attaccgccg taaggtcgat aaacttttgc tggtcgttgt
     1141 ttttcgcaac gttgatgaca atctgcttga taagcgctgt ctttaagtcc tgcaagcctt
     1201 ccacctgctc agccagggcc atgtcggcga atatcttgcg actttccctt acgctaccca
     1261 tcgcaaagaa gggagcaatg tcgaactcgt tggtttggat agacagcggc gtatcgccga
     1321 cacaatgtac aatcaaatgc tcgtaaatgc catcgtcgtt atagtccacg tctacatagc
     1381 actcgtacag ctcgacgtct ttagacgcgt tatcgccgtc gttcgggcgc atatggtcgt
     1441 ctgacagctc tttgttgatg tactcatcgg cggaggtgta cttcgtatcg cctgacgctt
     1501 ccagcgcttc gtcgacgttc tgataagtcc cgtcttgctc tttgcgcttg agatagtcac
     1561 cttttacgat tttccggtgt gctacgaact tgcacttttg gagtgtgcta gcttccggcg
     1621 tgaaacgcaa ttctgtgggc ggtacatact cgacgacagg ataattggcc gtgactttga
     1681 cgtggtcgaa ctgcacttca tacagatccg gcgcgtcttt cagctgcttg actttctgta
     1741 tctcgatttc gcccgacaac gacgcctgcg tgagcatcat cgcttgttgc atatcgttca
     1801 cgtcgaacat gagcttgtaa cgcgtgcggt cttcatcccg cttccaccat accttagcaa
     1861 cgcctagatt cgtccccaat gcgtcatcaa tgacgtcgtt cacaagggac gtatagttgt
     1921 tcttgcgggt aagctggtat tcgacaaggt gctgtatgtt tgttgctgta tcatcgtttt
     1981 ggatggtact gccagcgata gtaacaggcg actcgttgcc aatgaagact tctacaaggc
     2041 tgggcttcat ccactcaacg atgttattga agtccgtgct gacgaattta ctctttttcg
     2101 agatgttcgg cagcttcttt tcgtacaagt cctggtcgcc gttgcggagc ttgcggcggt
     2161 gtatcagttt cggctctacg gtaccctcgt agtatttctt tgcgacgtcg ataccgtctt
     2221 tgacgctcat catgatcttc ttgatttcgt cgtcgttgag cgtgtccagg gataccggct
     2281 gttcttccgg ctctgcctgc tgtaacagcc attccgtcac gctcatctgc tgcggggcgt
     2341 ctcggccgaa caatccgccc gtgtcctgtg cggcggacaa gctctgattt aaatcctcca
     2401 tctcatcacc tcgattgata gctaaataag gcccccggtg gggctatggg gcaatgcgcg
     2461 ggcttgaacc gcgatagctt atagctggtt tactttaacc tacactgcca tgagtggcgg
     2521 ggtcgtcagc cccgccgatg atagaaagga gcatgttacc gcggagtcgc ccttgtccgg
     2581 tgtggcgtcg ctccacgcgt ttacatgtat ccggtccggt gcatcttgcc acgggtcatc
     2641 cgcttccact tgtctgccat ggacgtgttg tcccggtaga gcttggcaca aaggtatgcc
     2701 agacagtcca ttagatggct gtattcgttc ttttccggct catccagcgt ccgcccggct
     2761 acgactttac gatgataccc gcccgtaaat gcttcgatga gcatctgaca gcgcgggtcc
     2821 agctgtaaga gcggtttccc gtcgggcgta agtgtcgtga gatagtaccg taccgcttca
     2881 ctacggcctg tttgcgtgag ctcgcccggc tctacgataa tgccgtagcg gtcacggagt
     2941 atctcgttcg cggtcttctc gtcgctctgc gcgcgctggt tgcccgccgg gtcgccgact
     3001 gccgtgtact cgtacccgct gtaaaacgtc tgtaattccg cttgtacggc acggccgtgg
     3061 gccaacatcc cgcaatccca ggactgcagt tccgacagga tgagcagttg cccctttgcc
     3121 gtcgtctgtg caatgatggt agccggggta agcccatagt caaacgtcag gagctgaggc
     3181 cgcccgtcaa gctggtgtaa ttgttagtta acgaattggc ggttgtaatc acagtccggg
     3241 ttgtcgttcg gct
//
LOCUS       DAWCYS010000192         3232 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1545_length_3232_cov_4.193264, whole genome shotgun sequence.
ACCESSION   DAWCYS010000192 DAWCYS010000000
VERSION     DAWCYS010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_3232_cov_4.193264"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          620 a          886 c          960 g          766 t
ORIGIN      
        1 ggtatagggg atccccatca tttcacacaa gccctggatg acaccgtctt tgccgtagcg
       61 gccgtgaagg gcgttgaaga cgatgtcgat acccttttcg gtcaaggtct tggcgaattc
      121 tttcggtacg agttcgatcg tttcgatgtg atatcccttt ttcctcaaag cgtcagcaat
      181 ggcagcccca gtgcggcgcg atacttctgc ttctgtcgaa ggaccgccga cgactacggc
      241 aattttttta tctttcatgt cttccatttc cttatttctc catttcttta gcttcagcga
      301 gataggcttc gccgacttta taaatgtctc cggcccccat ggtgatgacc aggtcgagag
      361 gccgggaatt ttcctttaaa taagccacta agtcttcttt gtgcgggata taggtgacat
      421 cttgtcccgt cgtttccttg accttcttgg caatgagttc ccctgataca ccggggatgg
      481 ggtcttcacc ggcactgtag acgtcggtga cgatgagttt atcggctttg gcaaaggcgc
      541 tgccgtactg gtcgaggagc agcttcgtcc gcgtgtagcg gtgcggctgg aagaggcaga
      601 cgacgcggtg cgtccccata tcgcgggcag ccgtcaaggt cgcattgatt tccgtcggat
      661 ggtgggcata gtcgtcgacg acccagacgt caccgacgaa gcccttggtc tggaaacgcc
      721 gtttggcgcc gtggaaatgg ctcatggccg acgcgatgcc gtcgaagggc aggccgcagg
      781 catcggcgac ggctgtcgtc gccagggcgt tgaggacgtt gtggcgtccc ggtacgttga
      841 ggaccatatc ccccatcttt tcgccgtgct tatagacgga aaaatggagc ttgcccttga
      901 ggtaatgcag gtcgctggcc tgatagtccg ccggctgttc gatgccgtaa gatacgtagc
      961 ggcggcccgt ctgcggggcc agggcgcgga tcttgtcgtt ttcaaagcac aagacggcca
     1021 ggccttcttc agggtcgagc ttctggatga actgcttgaa agcggcgatg acgttttcca
     1081 gcgagccgta atggtccata tggtcggcgt cgatattggt gacgacggcg atataagggt
     1141 tctgcttgag gaaagtccca tcgctttcat cagcttcggc gatgacatag gcgcctttcc
     1201 ccaggatgga attgctatgc aggtaatcga cttcgccgcc gatgacgatg gtcgggtcct
     1261 gatgacattc ttcaaagacc tggcccagca tggacgatgt cgtcgtcttg ccgtgggcac
     1321 cggctacgga aatccccttc ccccagctca tgatatggac cagggcatcc gagcgatgga
     1381 tgacaggaat accgcggcgg cgcgcttcgg ccagttccac attggtatcg cggatagccg
     1441 atgaaatgac gacgacctgg gcgtcctgca ggttttccgg acgctggccg atacagatac
     1501 gggccccttt tttggcgaac tgttccgtta ccggagacgc tttgaggtcc gaaccggaca
     1561 catgatatcc tttttcaatg aaaacattgg caattgcccg catcccagcg ccggcaatgc
     1621 cgataaaatg aacgttggta acatcgttca gattaaccat aggtttattc cacacccttc
     1681 ttttcctctt acggtcgacg tttgcgggca atcgacaagg ccagttcgtc aatatcgtgt
     1741 gccgcctgtg gcttgcccat ggagcggctg gctttcgcca tcgctgccag tgccttcgga
     1801 tcgtctttta aatgaatgat ttcttctaac aattcccggc ctgtcagcat cttgtcgagg
     1861 atcatcttgg cagcgccgca catgaccagg gcctgggcat tgtagcgctg gtggtcttcg
     1921 gctgcatagg gatagggaat gaggatggac ggcacgccgc ggacggtcag ttcagccaga
     1981 ccgacggcac cggcccggta gacggccagg tcgcaggcgg ccagggcgtc gggcatatga
     2041 tgcaggtacg ggatgatacg gcttcccttg ccatagcggc cgcggcattc gatgcccggc
     2101 agctgcttga cgacgcggtc gtattcatgg tcccccgtga cgtggagaat ctggatatgg
     2161 tcgtcgttgc ggaaatactt gtggacttcg atcatcgccg tgttgatgct gcgggcgccg
     2221 cggctgccgc cggcgacgag gagcgtaaac ttatccgggt ccaggcccag ggccttgcgc
     2281 ccttcttccc gcgtcaccgt gaggatatcc ttgcggacgg gattgcccgt atagaccagg
     2341 atatccttgc gcttgaaacg ggccgctgcc tcttcatagc ccagggctac gcggttgacg
     2401 aagcggctca aaatcgtatt ggtaacgccg gggatgacgt tctgttcctg tatcagtgtg
     2461 ggcacaccac tcaggctggc tgccaaaagg accggaccgc agacgaaacc gcccgtcccg
     2521 atgacgacgt ccggtttgaa tttgtggata atgccttctg ccttgatcag actgcctgcc
     2581 gtcttgccca gggtgacgag gttgcggacg gaaatcttcc gttccaggcc ccgcacgtcg
     2641 agggtgacga aaggcaagcc ttctttaggg atcaatgtat tttccaggcc gattttggag
     2701 ccgacgtaga gaaattcggt gggctcaatg tcggcaatcg cccgggcaat ggtaatcgcc
     2761 gggtagatat ggccgcctgt gccacctccg gaaataatga ctcgacgcat gttcctgcct
     2821 cctgttgttt cttctaaata tcgttaaatg gcgttgacga tgcccttgaa gacacgtccg
     2881 cgctgttcaa aattatcata catgtcaaag ctcgagcaag ccggcgacaa gaggacggcc
     2941 tgcggtgcct tggcaatttt gcgggccagc atgatggccc cttccatgct gtaaccagtg
     3001 cggcggatat cctggatacc cgccttcttg gcagcggctt cgacgcgttc ggccgcagcg
     3061 ccgatgagga tcagcgtatc gacgcgtttc gccgccaagg ccatgaagtc atcgaggggc
     3121 gtcttcttgt catagccgcc ggcaatgagg atgaggtgcc cctgcgggaa ggcttccagg
     3181 gccttcatgg acgcatcgac gttggtcccc ttcgagtcgt tgtaataggt ga
//
LOCUS       DAWCYS010000193         3229 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1548_length_3229_cov_8.687776, whole genome shotgun sequence.
ACCESSION   DAWCYS010000193 DAWCYS010000000
VERSION     DAWCYS010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_3229_cov_8.687776"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          869 a          842 c          796 g          722 t
ORIGIN      
        1 aacggcgacg attactggct tgaccaataa aacaacggat tacaccggtt ttgcaaacgg
       61 cagtggccgg gcagctacgg aagaacagct gaaggaagtt gcaggcaaag ctggtgaagc
      121 tatcgaaacg gctggcaaag gctggaacct gacgactaac ggtaaagaat ctagcaagac
      181 caacatcaaa ccgggcggca cggtagactt ctccagcagc aatgacaact tgctcatcag
      241 caatgctggc gcagacctct cgttctccct cagcgacaat ctcgacctga ctaagaatga
      301 ttctaagaag ggcagcgtca aagtcggtga aaatacgaca ttgacggatg gacttctcca
      361 agtcggcacg gatgtttccc tcacggccag tgctcttaac gtaggcacct cgtccttgac
      421 gacaagtgcc ctcatggttg gcggcttgac ctacatcaac tccaacggtc tcaatgccaa
      481 taaccgggtt atctccaacg tagcagctgg tatccttgat acggatgcgg tcaacgtcgg
      541 tcagctcaag tcggctatca gcggttcgca agtttccatc aaggctggtg atggcatcag
      601 tgtcgccaaa acgggcaacc aatacacaat caatgttaat attgaaggcg ttaccaataa
      661 ccaaggcaaa gtaacggttt cgacaggtga cagcacttcg acagaaagtg gttccgaatc
      721 cggttcgtcg ggcgctaaaa agtcccaagt catggacctc atgtatcgga cagagtctaa
      781 tgaagttgct ccagaaacga cgacagatac cggtaaagct atacaaattg aaagtattcc
      841 ggaagatatc aagcttgaag ctgatgataa ccaggctgcc agtgtcgtaa acggtgaaac
      901 tattaagatt gctggcgatg gtacgaatat ctcgacggca gctaccgtta ctggatcagg
      961 cagcatggat accatttccg tttccctgaa accggatatc caagtcgata gtgttaccat
     1021 caacaatggt ggcccgacca ttaataatac tggtatcgac atgaatggta agagcatcac
     1081 caatgttgaa aatattaccg tcaacaatgg gccgactatc aatagcagcg gtatcgacat
     1141 gaacagccag aagattacca acctggctga tggtgaaatt gctgaaggct cgacggacgc
     1201 tgtcaatggt ggccagctct acaacacgaa tcaggccgtc attgccaacg cggaaaacat
     1261 caacagtctg agccattcgc tcaacaagct ggatagccgc atcaatcgtg tcggtgctgg
     1321 cgcagccgct ctggcagccc tccacccgct cgattttgac ccggacaaca agtgggactt
     1381 cgcagctggt tacggcaact atgccggcgc caatgcagtc gctatcggta cctactatcg
     1441 cccgaacgaa aacaccatgt tcagcatcgg tggcagcttc ggcggcggcg aaaacatgat
     1501 caacgccggc gtcagcttca aactcggcag cggcggcagc ggtatcacga cgtccaagac
     1561 ggtcatggct aagaagatta aagaacaaga tgaactcctg aaagcacagg atgctaagat
     1621 gaaagaacaa gacgaaaaga ttgctaaact ggaagctctt gtagcccagc aaggcgaaat
     1681 gattcagcag gcactgggca agaaatagtg ccatagcgat gaggttgtcg caccaagtca
     1741 agattacttc aggcgacaac ctcatttttt ttcgcaggtc gcagttcgca aaaaaaatac
     1801 acggcccatg gccttgtgtg taggggctgt cccgatgagg gcagcccgct gcgaaacttt
     1861 gccacagcct attctgttat aatggtatgt ttgtaggggc tcgcacgtgg cgagtccgct
     1921 agagaaattt ggctataact tatccgagag caggctttta cgagccacga acaatgaacc
     1981 acggcctttc tctcggattc atatgcggcg ttccgccgcg acatactcat gatagtatta
     2041 cgaccatgta ccgtccgtca taaattccgg ctatgcctag gattcacggc gggacgcccg
     2101 ccactgctgt ccgttctggc agccgccctg cacctcccga tagtcggtac ctggcggacc
     2161 agaaaggctt tgggcgtccc ctacaaaatt tcccttttgg ttgtaaatgc aacagaaata
     2221 tttctttctt tttgttatac tttacgtgta ctcgatgaga gcagagaatt acgaatcacc
     2281 cattcaattt attatatgta tgaaatacag gaggttttca ccatgacaaa agaaaaacag
     2341 atgttttgtt tccagtgtga acagaccgct atgggtactg gctgtaccgg ttgtgccggt
     2401 gtttgtggca aatcggctgt tgaagccggc ttgcaggatg ttttgaccgg cgcccttgtt
     2461 ggtttggccc gcacctgtca gactgccgct ccgacggcta agacctatcg tactatcctc
     2521 gaaggcatgt tcacgaccat taccaacgtc aacttcaaca cgacgacgac ccaggctatg
     2581 atcgataaag ttcacgctga agccgctgcc ctttcgtcca ctcccgcagc tgactacgat
     2641 atgaaccaga tgtggaacgc tgacgaagat atccgttccc ttaaatcgct catcctcttt
     2701 ggtatgcgcg gcatggctgc ttatgcttat catgctctca tgctcggcaa atcgagcgaa
     2761 gaagtcgatg ctttcttcgt caaaggcttg tcggccatcg gcgaagactg gggcatggat
     2821 gaactcctcc cgattgtcat ggaaacaggc aaagtcaact tgacctgcat ggctctcctc
     2881 gatgaagcta atacgtctac ttatggtact ccggttccga cgacagttcc tttgacggtt
     2941 gaaaaaggcc cgttcatcgt tgtcaccggc cacgacctga aagacttgga actcctcctc
     3001 aaacagactg aaggcaaagg catcaatatc tatacgcacg gcgaaatgct cccggctcac
     3061 gcttatccgg aattgaagaa atacaaacac ctcaaaggca acttcggcac ggcttggcag
     3121 aaccagcaga aagaattcga cggcgttccc ggtgcattcc tgttcacgac gaactgcctc
     3181 atgccgcctc gtaaatccta catcgaccgc gtcttcacga cggaagtcg
//
LOCUS       DAWCYS010000194         3181 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1566_length_3181_cov_5.536788, whole genome shotgun sequence.
ACCESSION   DAWCYS010000194 DAWCYS010000000
VERSION     DAWCYS010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_3181_cov_5.536788"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          867 a          876 c          839 g          599 t
ORIGIN      
        1 ccctcgacca ggaactgcag gccaaagtcg ccctcaagta gaacgagctc aacaacaggc
       61 tgaaacagcg gtatgattcg ctcgtccagg aaaagaagaa gacgacgcct gtcatttccc
      121 aggccgacac gttgaaaatc gtcaatctcc agttgaagct gaagaccctg tccctgtcta
      181 aagaagaacg ggcagcgaca gaggaacagc tccaaacctt gctcaaaggc agccaggctg
      241 acgtccaggt gaccaaccgg acagccgatg aaatcgcggc cctcatggca ccggataagg
      301 cccaggccaa gaaagagctc gatgactacg cccagcaggt caagaaagac ctggaaggac
      361 gcaaggcaga caaccagcag gtcttccagc agcagctggg cgtcttgcag gaccggccgg
      421 aaccggctgt gtggaacaag gaatggaaag ataaactcga tgcaaaagaa aaagaaatga
      481 aagatgtgaa agacgccatt atggcagata tccgcgataa agccgccgat gtcgcccagg
      541 aacagggctt tgagatgatc ttcgccaact acgaaggcat cggcacggct acggatgtta
      601 ccgatgatat tattgccaag ttggcataac aggaggcttt tagcaatgaa aaaagtttgg
      661 aagcgcttag gaatcgtcgc cgcaatgctg gccctgacgg gtatgctcgc cggctgtggc
      721 ggaaaagata atgtaggcgt cgtcgacatg tcgcgcgtac agaaagaagc gcccctggtc
      781 cagcagtata aacagaagac cgaagataaa cagaaatcca tcgaaaagga attgcaggac
      841 gcacagcagt ccatgtcggc tgaagatttc cagaagaaac agcagcaggc ccagcaggaa
      901 ctcaatattt tcggcgccag catgcagcgt cagttcatgt cggacatcca gagcaagtta
      961 ggcgacatcg ccaaggataa aaatgtcggc atcatcgtcg tcaaagaagc cgttccttcc
     1021 ggcggcatcg acgtaaccga cgatttaatt gccaaattgc agtaaaaaaa ggggttgtat
     1081 atcgtgaaga aaacagtaca ggaattagca gtattgctgg gcggcacggt catcggcaag
     1141 ggtgacgccg tcattgaaga cgtgaaaggc ctggccgaag ccggtctgca ggacattacc
     1201 tttgccgtcg atccgtatac ggaatatttg ccgcaggtcc atgccggtgc cgtcattgtc
     1261 gaaaaagaag ttcctgccgg agataatacg ctcgtcgtcg tagaaaatcc ccgcctggct
     1321 ttttcccagc tcctcgtgct gtttcatccc cgccagtcgg tcacgccggg catccatccg
     1381 acagccatca tcgacggcag cgcctctatc ggcgaagacg tagccatcat gccttatgcc
     1441 gtcatcggca agaacgtcga aatcggcaag ggctcgacga tttatcccta cgtcttttta
     1501 ggcgataatg tcaagatcgg cgaaggttcg gtcctctatc ccggtgccgt catccacgaa
     1561 aactgcgtcc tcggaaagaa cgccgtcatc cgggctcacg ccgtcatcgg cggcgaaggc
     1621 ttcggctttg ctacggaaaa gggcaagcat acgcggatcc cgcagatcgg caacgtcacc
     1681 ctcggcgacg atgtcgaaat cggcgcctgc acgtgtctcg ataatgcaac cctgggttcg
     1741 acgaaagtcg gcagggggac gaaaatcgac aacctcgtcc atctcggcca caacgtcgaa
     1801 atcggtgaag actgtttcct catcgcccag acgggcatcg ctggcagcac caaagccggc
     1861 aatcacgtca tctttgccgg tcagaccggc tgcactggcc acatcaccat tggcgacaat
     1921 gccgtctttg ccggcaaatc gggcattacg ggtaatgtca agggcaacca ggtctatgcc
     1981 gggttcccca tccggcctca catggaatgg agccgtaccc aggtctacct gaaacacctg
     2041 ccggaaatgg ccaagaccgt caaagccttg cagaaaaaaa tcgctgaact ggaaaagaaa
     2101 ttagaacaac agtaagagga tttgcctatg tacaccttta tgaaatgtct gagctggatc
     2161 atctgccaca ttccagaagg ctgtcgccgg gccctaggca cctttttagg tgcctttttc
     2221 tggactttcg ttcccaagaa gcgcaaggtc ctggcccagc agcagatttt ggactgcggt
     2281 ctgaccgatg atccggaaaa ggccatggcc atcgccaagg ccagtacggt ccggttcggg
     2341 cccatgatca tcgaagtcct gtcgtatccg cgctatacga aggagatgct cgacgaaaag
     2401 attacctggc acggcaagga atacctggac gaactcaaag ccagcggcga aggggccgtc
     2461 ttcatggcat cccatgccgg caactgggaa ctcctgggag ctgtcctggc catgaatggc
     2521 tatcccctca tctccgttgc tcaggaacag aacagcaaga gcgccgatac cttcatcaac
     2581 gagtaccggg ccatgatgaa gcagcacgtc acttacaaga cgggcatccg cgacatggtc
     2641 cgcttcctcc gcgacggcca ctacatcggc ctgctcatgg accaggaccc gggctatacc
     2701 ggcatcatgg tcaaactctt tggcatggac accctgacgg ctgacggacc ggccaagatg
     2761 gccggcatcg ccaattaccc catcgtgtcg accttcatcc acgaagaccg gccgtaccac
     2821 cacgtcgtcg aagtcctgcc gcccatcaag ccgtacacag cggaccacaa gctctcgaaa
     2881 gaggaaaaga accgcatcat gtacgaaacg acacaggaac tcaacgaccg cctggaagcc
     2941 catatccgca agtatccaga agactggttc tggctgcaca atcgctggaa atggactaaa
     3001 cggtataaaa taaaacaagc tgaaaagggg gctggcgtat gaaggcttat gccatcatgc
     3061 gtagtcacta ttacttcgta gtttgtagtt cggagtttgc agtttgtaga aaatggctct
     3121 aaatttaaaa ccattcacta aaaactacaa actgcaaact taatactcaa aaagaggctg
     3181 t
//
LOCUS       DAWCYS010000195         3178 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1568_length_3178_cov_4.742555, whole genome shotgun sequence.
ACCESSION   DAWCYS010000195 DAWCYS010000000
VERSION     DAWCYS010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_3178_cov_4.742555"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          686 a         1071 c          857 g          564 t
ORIGIN      
        1 cccgcaacgc ctggatcgac gaaaaacgcc tcgtcaggga agaactgacc agcatgaaac
       61 gcgccggcgc cgatatcatc atcacctacc acgccgtcga cgtcgccaag tggctgaaaa
      121 acggcgaagt cgaggcataa aatggacctc gtcatcctcg gcctcaatca taagaccatc
      181 cccgtcgcca tccgggaaca cttcactatg acacccgacg ccatccacga cgccctgcag
      241 catctcgacg attacggcgg cctgcgcgaa gccgtcatcc tgtcgacgtg caaccgcgcc
      301 gaagtctatg ccgtcgtcga cgacgtgcgc gataatgaac aggccctgcg cgacttcttc
      361 ctcgacgcag ccggccgtcc ggcttatgaa ccgtcctatt tctacagcta tacgggaaaa
      421 gcctgcatcc accacttgtt caacgtcgct gccagcctgg actcgctggt cctcggcgaa
      481 ggtcagatcc tcagccaggt caaacaggcc tacgtcatgg cccacgaaaa cggcgcgacc
      541 agtgccatcc tgaacatgct cttccagcag gccatcgcca cgggcaagcg ggtccggacg
      601 gaaacgcaca ttgcctacaa cgccgtatcc gtcagctata cagccgtcca gatggccgaa
      661 aaacagctcg gctccctgtc tggccggaat ctcctgctct acggcgccgg ccagatggcc
      721 cgcctgacgg cgcagaattt cttaggcaaa ggcgccggga aactgtacat cgtcaaccgc
      781 catctggaac gggcccagga actggccgct gacgtcggcg gccaggccgt ccactataaa
      841 gaagtccagt ccgtcgtctc cgacgtcgac atcatcatcg cctcgacggg ggcaccgcac
      901 tacgtcatca ccgtcccccg gctgcagcgc ttccaggaac tacgccaggg ccggcccctg
      961 ctgctcatcg acatcgccgt accgcgcgac gtcgacccgg ccgtgacccg tctgcccggc
     1021 gtcaccttat acaacatcga cgaccttcag gaagtcgtcc acgacaacga agctacgcgc
     1081 cagcgcgaag ccgtagccgc cagctatatc gtcgacgaag ccgtgaacac catgattgaa
     1141 cgctatcaat acctgtcggt ccgtcccgtc gtcctcagcc tgtcccagca ggctgaacgg
     1201 acacggcgcc atttcgtcaa acgagcccgc aacaaactgc ccgaactgac cgatgaccag
     1261 ttccgcgtcg tcgagaactt atcccatatc ctggtccgca aaatcctgcg tgctcccctg
     1321 cgccgcacgg tcgaagcagc caacacgcct gacgaagacc gggtcgtccg cgaactgcgg
     1381 aacgtcttca atttagacat caaagaataa ggaggccttt ccatgcttca cttagaaaaa
     1441 tcagctgccg cttttgaaga agcgcagacc ctcatgccgg gcggcgtcaa cagcccggtc
     1501 cgctcgtata accgcatcgg ctgcccgccg ccgttcattg cctgggccaa aggcagtacc
     1561 atctgcgaca tcgacggcaa ccagtatatc gactacgtcg gttcctgggg ccccatgatc
     1621 gtcggccacg cccatccccg cgtcgtcaag gccctgcagg aagcggccac ccgcggcacg
     1681 agctatggtg cgccgacgct gattgaatcg cagctggccc agttggtcaa agaaatctat
     1741 ccgtccatcg aaaaactgcg catggtcaac tccggtacgg aagcgacgat gagcgccatc
     1801 cgcgtcgccc ggggctacac gtgcctcgat aaaatcttta aattcatcgg ctgctatcac
     1861 ggccacggcg acagcctgct ggtcaaggcc ggttccggcg ccgctacctt cggtcagcct
     1921 gacagcccgg gcgtcacagc cggcacggcc aaagacacgc tgaccgtcgc ctataacgat
     1981 gccgacgcct tccgggaact catggaccgc tgcggcgacg acattgccgc cgtcatcgtc
     2041 gaacccattg ccggcaacat gggcctggtc ctgccggaag acggctactt gcagctcctg
     2101 cgcgatgaaa cgaagaaaca cggcacagtc ctcatcttcg acgaagtcat gtgcggcttc
     2161 cgcacggccc tgcacggcgc ccagagcatc tatcacatcg tcccggacat gacgaccttg
     2221 ggcaagatca tcggcggcgg cctgcccgtc gctgcctacg gcggccgcaa ggacatcatg
     2281 gactgcgtct cgccggccgg cccggtctat caggccggta ccctcagcgg caatcccctg
     2341 gccatggcag ccggattgga aacactgcgc atcatcacgg aagacccgga ttacgtcgaa
     2401 aaagcctgcg ccaaaacgaa aatcctgaca gacggcctca aagccgctgc tgacgaagcc
     2461 ggcgccgccg tccaggtcca tcgggccggc accatgttca gcgtcttctt cagcgccgtc
     2521 ccggtcacca actatgccac atccgaagcc agtgaccagg aagccttcca gcgctacttc
     2581 ctgtccatgc tggaacaagg catctacctg gcaccgtccc agtttgaaac gaacttcgtc
     2641 tccggcgtcc acagcgacga agacatcgaa aaaaccataa ccgccgcccg cacagccttt
     2701 gaaaaagcag tggttcgtgg ctagtggcta gtggttaggt ctcgcagcct gatggctgca
     2761 tcgtaggggc cgtcccaaag ggcggcccgg ctctcctgct aggagagctg tcagcgcagc
     2821 tgactgagag gtttatcggc ggcctgcggt atcaggttgc ggaattccca caagcgggcc
     2881 atccatcctc agctgtccgt ttcagcaacc gtcgggtgcc ttccatgggt cggcacccga
     2941 cgggctgaaa aagcttgggg acgaccccta cggcaaaaag ggctgtcgca cgaagcaaaa
     3001 attgcttaat gcgacagccc tttttttgag ttttaagttg aaagtttgat gtctgatgtg
     3061 ggtcgccacg tgggcggtcc gtttggaatt ttgatgattc tcctagtaga ctggttagga
     3121 aaatcgtgca tatgcggaag cgggctcgcc ccgtgcgcgc ccctactgca gcttgcga
//
LOCUS       DAWCYS010000196         3168 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1575_length_3168_cov_9.230967, whole genome shotgun sequence.
ACCESSION   DAWCYS010000196 DAWCYS010000000
VERSION     DAWCYS010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_3168_cov_9.230967"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          631 a          812 c          899 g          826 t
ORIGIN      
        1 gccccgacag cttattcagt atactactgt gtaaagagat tgtcaaggat tttttcaaat
       61 ccttgacgcc ggccgccggt cgccggtcgc aaacccacgc cacatatgcc gatcaagtat
      121 atcccccatt gttcacagcg ggccgtcaaa ggctcccttc ataggggagc tggccgccaa
      181 aggcggtctg agaggttaaa ggacgtcccc tacgatggcc ccggttaggg cgtaagccgc
      241 ccctgcgaag gggaggggga ccgcttgcgg tggaggggtt ctcctctacg agccgagggt
      301 tactcgaagc gatgttgtca gtctgagtct gtcgcggcgg aacgccgcat ctgaattcga
      361 ggcggcctgc ggcctgcgaa ctgcggtcgg cgcaaaaaaa aagaggcccg caggcctctt
      421 tttttattta aacaatttcg tctgttcttt ttcttcttcg tcgttacctt cgatggcaat
      481 ggtatcgagt tcgtcgttac cttcgatggc aatggtatcg agggcggcta ctttgtcgtc
      541 gccttccagt ttctggatct tgacgcccga tgtgttgcgg cctttcttga tgccaatgct
      601 ttcgatatcc gtgcggatga cgatgccatt cgacgtgatg agcatcagtt cctgcccttc
      661 atggacgact tcgacgccga cgatgtcgcc ggtcttcggc gtaatcttga agttcttgat
      721 gcctttgcca ccgcggagct ggactttgta ggcgtcgatg ttgttgcgct tgccaaagcc
      781 ttcttcgctg acagtcagga cctggcagtc cggttcgagg acgtcagccc cgatgaccat
      841 atcgccttca gacagtgtga tgccgcggac accgcgggta ttccggccca taggacggac
      901 atcgttttca ttgaagcaga tggccatgcc cagtttcgtc gccaagatga tcgtctggtc
      961 gccgttggtc aggcgcactt tggccaggtg gtcgccttcc ataagggaaa tagcgatgag
     1021 gccgttgcgg cggacgttct tgaattcatg aagaggggtc ttctttacca gccctttttc
     1081 ggtgaccatg aacaggttca tcgacgggtc gacctggtcg aggtcgatga gggcgttgac
     1141 tttttcgcca ttggacaagg gcaggatatt gatggcggca atgcccttgg agttacggct
     1201 gttggcttcc gggatttcgt aagccttcag gcgatagacg cggccgaagt tggtgaagaa
     1261 caggactgta ttccatgccg acgtatagag caggtggttt acggcgtctt cgtccttcgt
     1321 ccccatgccc ttgataccgc ggccgccttt attctgcgta tggtatacat tggcgttgat
     1381 acgcttcatg tagcctcggc gggtcaaggt gaggaccatg cgttcgttgg ggatcatgtc
     1441 ttcgatggag aaatccgacg agtcggctac gatctgtgtg cgccgttcgt cgccaaattt
     1501 cttcttggca tcgaggagtt ccgtctttac gatttccatg actttatgtt cgtcagccag
     1561 gacgctgcgc aggtaagcga tgcgtttttc caattcagca tattcttctt cgattttttc
     1621 tcgttccaga cccgtcaggc ggcgcaggcg catgtcgagg atggcgatgg cctgtttttc
     1681 gctgaggccg aacttgctca tcaaggattg tttggcaatg tcgtctgtcg ccgattcgcg
     1741 gatcgtcttg atgacttcgt cgatgtggtc cagggcgatg agcaggcctt ctaagatgtg
     1801 agcccgtgcc agggccttgt cgagttcaaa cttgctgcgg cgggtaatga catctttctg
     1861 atggtcgaga tagtagttca ggatctgttt gagtgtcagg acgcgcggat ggccgttgac
     1921 gagggccagc atgatgacgc cgaacgtttc ctggagctgc gtgtgtttat acaatttatt
     1981 caggacgata tcgggattga catcggcccg cagttcgacg acgatgcgca tacccttgcg
     2041 gtccgattca tcgcgcaggg ccgtgatgcc gtcgatttcc ttgttgcggg acaagtcggc
     2101 aatggactcg atgacccgcg ctttattgac ctgatagggg atttccgtca cgacgatgcg
     2161 gtgcttccct ttattcatcg gttcgatagt cgcacaggac cgcatcttga tactgccccg
     2221 gcccgtcgaa taggctttga tgatgccatc gcggcccatg attttagcac ccgtcgggaa
     2281 atcagggcct ttgattttgg tcatgatttc ttccaggctg gcttcagggt tatcgatgag
     2341 caggacacag ccgtcgatga cttcgcctaa attatgaggc ggaatattcg tcgccatgcc
     2401 gacggcgatc ccagccgagc cattgaccag gaggttcggg atcttcgacg gcaagaccgt
     2461 cggttcctgc agggagccgt cgtagttggg catgaaatcg accgtttcct tgtcgatatc
     2521 ttccagcatt tcgccggtaa tcttcgccat gcggacttcg gtataacgca tagccgcagc
     2581 cgagtcacca tcgacggaac cgaagttgcc gtgaccatcg actaaaggat agcgcgtcga
     2641 gaaatcctgg gccaggcgga cggtcgcatc atagacggag ctgtcaccat gggggtgata
     2701 tttacctaaa acttcgccga caatacgggc cgatttctta tagggcttgt tcggcgtcat
     2761 gcccgcttca tgcatggcat agagaatacg ccgatggacc ggctttagcc catcgcgtac
     2821 atccggcagg gcacgagtga tgatgacact catggcatag tcgatgtacg acgtctgcat
     2881 ttcgctttcc agacaaaccg gcacaatttt atctacttgt tgttcgtcca caaatttcac
     2941 ctcatataca aactgaaaat aactgccggc cagcaaaaga cccgcagtcc gaatcgttgt
     3001 cgtctatatg gcagcccgtc aaatgaacaa atgggcgcat acgattatat tataacaaat
     3061 atttccattt ttctctagag gcgcaaaaaa acttgtttcc gcctgccttt ttaggctgag
     3121 ggaaacaagt tttttagtgg ctagtggcta ggggttaggg gttagggg
//
LOCUS       DAWCYS010000197         3164 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1581_length_3164_cov_5.495979, whole genome shotgun sequence.
ACCESSION   DAWCYS010000197 DAWCYS010000000
VERSION     DAWCYS010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1581_length_3164_cov_5.495979"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          597 a          845 c          944 g          778 t
ORIGIN      
        1 tacttattgc tcatagccga gcattcgatg tgccagccca gacggccttt gccccagggg
       61 ctgtcccacg acggttcgcc gggcttagca cctttccaga gggcgaagtc catgggattg
      121 tgcttgcggt cgtcgacttc gatacgggcg ccggccatca tgtcgtcgag gctgcgaccg
      181 ctcaattcgc cgtagtcggc gaacttttcg acgctgtaat agacgttgtg gtcgatgacg
      241 taggcatagc ctttgtcgat gaggttctgg accatgttga tgatttcatc catgtgttcc
      301 gatacgcggg gatacttgtc ggcccggcgg atgtggagct tgtccatgac gtcgaagtac
      361 gatgcgatat agcggttgga aatggtatcc cacgtgacgc cttcgccgtt ggcagcgttg
      421 atgatcttat cgtcgacgtc ggtgaagttc tggatgtgcg tgacttcata gcccaagtgt
      481 tccagatagc gcttgatgac gtcccacgtg acgaaaggac gggcattgcc catgtgggaa
      541 tggttgtacg gcgtaatgcc gcagacatac atcttgacct ggcccggcgt aacgggcacg
      601 aagatttctt ttttacgggt caatgtatta tatactttca gttctctcat acggtaacct
      661 ccctgaaatt tgaacaaaaa ggccaccatc ccctatgaca gagacggtgg cccgtggttc
      721 cactctgttt ttacggcagt gccgtaatct ttactgcgca gtaacgggcg cccccggacc
      781 ggattactcc tttcacccgg cctgctcaca aaggcagcat tgcagtttta tgacctggaa
      841 aggctctcag ccatgaccct tcttctctgt cggatgccac tgcaactcat tttgatcatc
      901 gcagattctt atttgataca tattattagt taatacgcat gtatatagta cccaataatc
      961 gggctcatgt caagcccttt atgccaacgt ttcgtccaga cgggccagga ctttgtcgcg
     1021 gcccatgagg gcgacgatga cgtttaggtc cgggccgtgc atgacgccgg tgacggcgac
     1081 gcggataggc atgtagacga acttgccctt gaccttgagg cccttctgga ttttcttgaa
     1141 caggggctgt accgtgtcgg gcgtgacgtc gtcgagggca gccagttcat cccggaacat
     1201 cttgaggacg gacggagccg tttcttcttt gaggactgct gcggtttctt cattttccgg
     1261 cgcatagtcg tcggtgaaga agaccttggc agcgtcgacg atctgagcgc cgtactggac
     1321 gtgttcgcgc atggcggcgc agatggcttt caaccaggca gcttcctttt cgtccggatt
     1381 ttcgctggca taacctgcct tctgcaggtg cggcaggcag acggcataga gctggtcttc
     1441 atcgagctgc ttcatgtaat ggaagttgat ccagttgagc ttttcgatgt cgaagacggc
     1501 cgggttcttg gcgacgcggt cgagggtgaa ctgttcgatg agttcatcgc ggctgaagat
     1561 ttcctgatcg ccttccggcg tccagccgag gagggccagg aagttgacca gtgcgtcggg
     1621 caggtagccc atatcgcggt actgctggac ggatgtagcg ccatggcgct tgctcatctt
     1681 cttcttgtcg gcgccgagga tgagcgatac gtgagcgaac tgcggggctt caaagcccaa
     1741 tgccttgtag atgaccagct ggcgcggcgt gttggacaag tgttcttccg cacggatgac
     1801 gtgggtgatg tgcatgaggg catcgtcgag gacgacgcag tagttataga ccgggatgcc
     1861 gtcggatttg acgatgacga agtcgccgac gttgttggaa tcgaaggata cgtgaccgcg
     1921 gacgaggtcg tccagttcaa tcgtttcgtt cttcggcgtc ttgatgcgga tgacgtgggg
     1981 acggccttcc ttgaggtact gttcgaccgt ttctttgggc aggtcagcgc atttgccgga
     2041 gtagaccggc gtttcccctt tggccagctg agcctgtttt tccgcttcca gttcttccgg
     2101 cgtgcagaag cagtagtagg ccttgccttc ggccaagagc ttttccgtat atttctgata
     2161 aatgcccagg cgttcagtct ggcggtacgg gccatcggga ccgccgacgt cgatgccttc
     2221 atcccaggtg atgcccagcc atttcagggc ttccttgatg ttttcttccg attccgacgt
     2281 cgaacgcttg cggtccgtat cttcgatgcg gacgacgaac ttaccgtagt tgtgcttggc
     2341 aacgaggtag ttgaacagtg ccgaacgggc accgccgatg tggaacgggc ctgtcgggct
     2401 cggagcaaaa cgaacgcgta cttcctgtga cataaaaaat tcctcctaaa gactatatat
     2461 atgtattatt gatagctaag gaccatggct gccgcctggg cggccatgcc ttcttcccgg
     2521 ccgacgaacc ccagtttctc tgtcgtcgtc gccttgacgg agacagcagt cttggggata
     2581 cgcagggcct cggcaatatt agcgatcatc tgcggcacat agggcttgat tttaggccgt
     2641 tgtgccaata aggtcacgtc gaggttgacg atgcggcagc atttctcccg cagcagatgg
     2701 cggacctttt ccaagaggac caggctggaa atatccttga aggcctggtc cgtatcggga
     2761 aaatacgtcc cgatatcgcg gagcccggcg gctcccagca gggcgtccat gatggcgtgg
     2821 atgacgacgt cggcatcgga atggccatcg aggccccgtt caaagggaat ctcgacgccg
     2881 cccaggacca ggcgccgccc ttctttcagg cggtggacgt catagcccgt accgatgcgc
     2941 tggacttccc tccccttcat gtacagggac aggatgggaa tgtcgtcgcg cgtcgtcacc
     3001 ttgcggttct tctcgctccc ttcgaggatg accacgggta tacccagggc ttcgacgagg
     3061 gatgcgtcat ctgtcgcctg gatgccctcg gtccgggcct gctcatgggc ttccatgagc
     3121 aggtcgcggt gaaagatctg cggcgtctgg gcggcgacca ggcg
//
LOCUS       DAWCYS010000198         3155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1586_length_3155_cov_5.420000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000198 DAWCYS010000000
VERSION     DAWCYS010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_3155_cov_5.420000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          768 a          894 c          763 g          730 t
ORIGIN      
        1 ccccgcgccg cgtctgcaat tcgtcagccg atacgctcat cttccccggt tcacgcaggt
       61 ccttttccca ctgggccact ttgtcggcag cttctttggc gacgtagaac aagaagcgtt
      121 caaaattgct cgacaagagg atgtccatgg acgggctcga cgtcgtgtag aagtcgcggt
      181 tcatgtcata gacgccggtt tcaaagaagt cggccaggac cttgttctgg ttagatgcgc
      241 aaatcaactt gccgataggc acgcccatct tcttggcaaa atagccagcc aggatattgc
      301 cgaagttccc cgtcgggacg atgacatcga acgtttcgcc ttcgctgatg gcgccgttat
      361 cgacgaggga gacatacgtc gatacgtaat aggcgacctg cgggaacaag cggccgatgt
      421 tgatggaatt ggcgctggag aagaggacgc ccttgtcgtt ggcataggcg ttgacttcag
      481 cgctagtaaa gacgcgtttc aaaaagctct gggcgtcatc gaaattgcca taaatggccg
      541 ataccttgac gttattccct tcctgcttct gcatctgctg cttctgcatg gggctgacgc
      601 cttcgctggg ataaaagacc atgatattcg taccggccac gtccttgaag ccttccaggg
      661 cggccttgcc cgtatcaccg ctggtcgccg tcaggatgag gatatcccgg tcctcttttt
      721 cctgcttctt ggcagccgtc agtaaatacg ggaacaagga caaagccagg tccttgaagg
      781 ccaacgtccg gccgtggaac aattcggcaa tggacaaatc cgtatcgatg gaatggagcg
      841 gtacgatgcg gtcatcgcgg aaggtcatat cattataagc cgaagcaatc atctgcttcc
      901 gttcttctgc cgtgaaattc gtaaagaaat gggacaatac ggcttcagca atctcctgat
      961 aagacttgcc agccagttct ttataggaca ggaccttttc cgggaacgat tccggcacat
     1021 agagccctcc gtccgcagcc agtccatgca ggagcgcata agacggcgaa acacattcgt
     1081 cagatcgtgt acttctaaaa ttcatcgatg aaatccccct tttcagctaa cctcatagaa
     1141 tagctataag tatttatatt ataaagggaa atagccacat tgtccactat tctgtgaatt
     1201 ggcctaacgg ccccgcagtt cgcagttcgc ctcggactca aaatgccgct ttgcggcaac
     1261 aaactcagaa tgacgacatc gcctcgagtg aacctcagct cgtaaaaaag gccccaagtg
     1321 gggccttcgt aggggacgtc ctgaacaagg cgtccagcct ctgctgtccg ttttggcaac
     1381 cgtcctgcgc cttccgctgg tcggcacctg acaggccaaa aaggccacgt gaatctaggc
     1441 tttgacagcc accccttcca gatgcaggag agcgattttt ttatcgatgc cgcctgcata
     1501 gcctgtggct ctgccattgg cacctaagac gcgatggcag ggaatgatga tggaaatggg
     1561 gttatgccct acggcgccgc cgatggcctg agccgacatc ttctttcccg tccgttcttc
     1621 aatctgttta gccagatcac cataggtcgt cgtctgacca tagggaatct ggcagagcag
     1681 ataccagacg aggcggcgga acgcactgcc ctgcggcgcc aggggcagcg tcataatagc
     1741 cggcatccgc ccggcaaaat aatcatccag ccagtgcgcc gccttgcaca ggatgggcgt
     1801 atcctcttca cgcaggactt ccccctgcaa ggcaccataa caatacttct gcccttcaaa
     1861 ccaggccccg accagcgccg tatcactagc cgccaaaatc atcggaccca acggcgattc
     1921 atacatcatt ttgtagtaca tttgtttcac ctcttgtatc tatcacaatc aaggcaatgc
     1981 gttgccctgg gtaatgcgcc aaatagcgac ggtacgagcc gcctctatgg tatctttcaa
     2041 attcggatcc tgcaaagcct gttcgtcacc gatatgacca tcttcaatat ataatttatg
     2101 gttaaaagcc ttgtcggact gcatcaaagg aggtaataag gattcatacg tacttccagc
     2161 cggcaaaatc aattctgcca aagttggtgc aatctgcaaa tgacttcctg ccgtctggtc
     2221 tgtaaataaa tccggcgtca cgccatcacc ataaacataa cagggaatac cgctcaaagc
     2281 ccacaaggat acgtctgtcg caaaattgaa acgttctgcg tggtcacccg tcacgacaaa
     2341 tagtgccgac gggtcatccc cttcgacggc cttgatgaat tttcccataa cgtcgtcagc
     2401 ataccaaata tggcctaact ggtctatcgt ctttttatca ctgggaatcg acggtggcag
     2461 tttagctgtc actgcatccc gtgggaaccc atgggcatcg acatcgtaag cgaagggcgg
     2521 atgattactc gtcgtcaaga tgaaatagaa cgtatcaccc gtatctttct gcatctgctc
     2581 ccggatagcc tcaaaaaata ttccatccgg tgcgccccag gacgaacttt ctccttggtc
     2641 tggtaattca tcggcacaat gaaactcatc aaaaccttca cgcaaagtga aatgctcgat
     2701 atcctgccag ctccgcagtc cgccatacca gaacacggtc ttgtacccca tctttttcat
     2761 gacagcgccg atacctaagc catcgacatc gccatgacgg cccatcatat aattgacata
     2821 aagcccgaca tccggtaagc cggtcaagaa cccattgagg gaggtcatag tcccattgcc
     2881 attcgccaag aaatgatagg tatgcgctcc tttcgcttcc agccattcgc cagtacgggc
     2941 caatcccaaa tcgcggtatt ccggcagcaa cggccagagt gcataatttt ccccgaggat
     3001 gacgacgaca tgacgcggac gctgcgccaa agttttccca acggttttac gagtaaaggc
     3061 ttcatccagc gtggccgcct gcggattgcc gcccaggact tgaatcgcct ggcgcagctc
     3121 ttcaggtgct actgaacgca aggctcgttc cgtag
//
LOCUS       DAWCYS010000199         3140 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1595_length_3140_cov_7.100810, whole genome shotgun sequence.
ACCESSION   DAWCYS010000199 DAWCYS010000000
VERSION     DAWCYS010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_3140_cov_7.100810"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          682 a          888 c          796 g          774 t
ORIGIN      
        1 tacttcaaag tgtggttacg cgatatttat ttgttcaaaa agttgtaaac tgctgcttta
       61 atataaattt taatataaat atagttttag aacaagttcc agcgtcccat gtacaaggcg
      121 accagggagc ggataaacag acagacgaac aagatgtagg tcaggatata caaaaagttt
      181 tcaaagtcat ccatatgttc cgacttctcg tggacatggc ggaaatggtt caagagaaaa
      241 tgtcccatcc gatggtcgct tcctttcctt attctccgcc gtgcggaaat aattcatgaa
      301 cgcgccgggc cgactcttcg tctttgctga tgcccttgag cagggtccgt tcggaatttt
      361 ccatgtgctt cttggcgact ttgcgggcca tggcactgtt gtgatcggca atgttttcca
      421 ccatctgccg atgttcttcc cacgtcgctg ccaggcggcc gggatagtgc atggaaatgg
      481 agcggaagcg cagcatctgt tcgcgcagat tattgagtat atcgacgagg cgctggttcc
      541 ggctggcatg atagaggacg tcatggaaac ggacgtcggc atcgacgatc ttatcgaatt
      601 cgtcgttgtc gatatattcg ttgatttcga cgaggatccg ttccaggtat tccagttcgt
      661 ccggcgtgat ccgttcagct gccaggccgg ctgccagttc ttccagggcg ctgcggatct
      721 cgaagacctg ggcgatatcc ttcaaggaaa tatcggcgac atacgtcccg cgccggggaa
      781 ccatgacaac gaagccttcc agttccaatt tgcggatggc ttcgcggatc ggcgtccggc
      841 tgacgccgag ttcatcggcc agctggattt ccatgagccg ttcccccggt ttgagtacgc
      901 cgtccttgat ggcctggcgc agggtttcgc tgacgacttc gcgcagcggc ttataagcat
      961 ctaatttaat cggttctaat gcatgttgtc ttttcgtggt tttcatcgtt ctatctctca
     1021 ccttccactc atttcactat ggccgtcatg tatacgtcga catcatgcca cgacgccgtc
     1081 tggctgcgga cggtctcttc ctgtccaggc gggaccaggg cgaacaccgt cggcccactg
     1141 ccggccatga gcggctgcag gcctaacgcg gtcaggcgct gctgacaatc ggcgacttgc
     1201 ggcacattgg gaatgatcag ttcctcaaag gtattcccca tggccgccgt caatccctgg
     1261 aaatcgccgg cccggacggc ctgctcgacg gcatcgacag cgacgggcgc cgtctggatc
     1321 cggctgtcga acagggcata ggcctgtttg gtctgtacgg gcacgcgggg atggacgatg
     1381 accacggacc acgccggcag gggcggcagg cgtttcagtt cctcgccgat accggtgccg
     1441 cgcatggtgc cgccacagat gcagaaaggc acgtcggcac cgagaccggc accgagacgg
     1501 cagagttctg tttctggcag ctccagcccc cagaggcggt tgaggccgcg caggacagcg
     1561 gcgcaatcgg tactgccgcc ggccagtccg gcggcgatgg ggatatgctt ttcgatatgg
     1621 atggtcactt gttgattgtc ctttttatac ggacaaagtg ccttccaagc cttataggcc
     1681 agattcgttt cgtcgcaggc cagcgacggc tgcgaacaag tgagctcgaa cgcatcggct
     1741 tcgctcacgg tcacggtatc gcaaaggcct atcgattgaa agatagtatc aatattgtga
     1801 taaccatcgg cacgacggtc agttatggcc agggccagat taattttccc atatcctttt
     1861 tctactatat tcatcatact ttcactccgt gcataaaatg tgcattgtct tattgattaa
     1921 aattttccct atgttataat gagacgacag cagagaacag cgccctttgc gcatcctctg
     1981 tgaatgcaca ctttcatcat tatactataa tgattatacc gttttttcct ttactttaca
     2041 attcttcatt caacacggag gaaaaaaaat tatgaaaacc ctgacttcac ctaccctttg
     2101 ggaattagcc gtcccctacc tggcgtcctt tgtcggcagt ctggtcgtcg ccatcggcat
     2161 gaatgccttc ttcatcccct tccacttatt gagcagcggc ctcagcgggg tagctatcat
     2221 gttctacttc ctgaccggcc tccccgtcgg cattggcatc ttcatcctca acatcccgat
     2281 catgatcgcc tgctacaaat tcatgggccg cagctataca gtcctaagca tcgtcggtac
     2341 aatcatgctc tcggtcctgg tcgatgtcac gtccttcctg tcgacgtgga acatcatgca
     2401 cgacgccatg ctgtcatcca ttaccggcgg tatcttgtcg ggcatcggct tcggcatcat
     2461 ctacaaatac aatggcaaca gcggcggcct ggacgtcatc ggtgccatcg tcaagaaata
     2521 ttactccctg gaaatgggta cggtcacgat gatcctcaat ctggtcatcc tcctggcagc
     2581 cgcctggctc ttctcgctgg aacgggccgt tctgaccttc gtcgctatct acatcgccgc
     2641 tttcatcacc aacaaagtcg tcatcggcct gaaacagcga aaaagcgtca tcatcatctc
     2701 caaccgggcc acgcctatcg cccaggtcct catgcgctac gtcggccacg gcgctaccta
     2761 cctgcacggc cagggcgcct ataccatgca ggataaacgg gtcatctatg ccgtcatcaa
     2821 actgacggaa gtagccaagg tcaaggaaat cgtcaacaag ctcgacccgc aggccttcat
     2881 gattatcagc gatgcctcag aagtcgtagg ccgcggcttt acaacatcgc ccgtcaagta
     2941 ccaccagtat cccggcgatg ccctctccat gccgcataca aaaaagaaca tgccgccgga
     3001 atattagggg ctagtgattc atagcaacct ctcagaccgc ctttagcggc cagctcccct
     3061 atgatgggag ccccaaaaag gagctgtcgc atcgcgacag ctcctttcaa gttgttagtg
     3121 gctagttgtt agttgttagc
//
LOCUS       DAWCYS010000200         3135 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1598_length_3135_cov_5.089610, whole genome shotgun sequence.
ACCESSION   DAWCYS010000200 DAWCYS010000000
VERSION     DAWCYS010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_3135_cov_5.089610"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          764 a          917 c          809 g          645 t
ORIGIN      
        1 atttatttta aaattaattc atgatcatag gagagatgca gtatgaaaaa cgtagaacat
       61 tataaaaaag gttattttat gccccctgtt gtcgatatgt cctgggccca gaaagaatat
      121 cccgataaag cgcccatttg gtgcagcgtc gatttgcgcg atggcaacca ggccctcatc
      181 attcctatga gccttgaaga aaagattgaa ttctataaag tcttggtcgc catgggcttc
      241 aaggaaatcg aagtcggctt cccggctgcg tcggaaacgg aatttgaatt cctgcgctac
      301 ttagtcgacc acgacatgat tcccgatgac gtcaccgtcc aggtcctgac cgaggcccgg
      361 gaacacatca tccgcaagac attcgatgcc ctgaaaggcg tcaagaacgc catcggccac
      421 gtctacaatt ccacgtcctt cgcccagcgc cagcaggtct tccgcaaatc gaaggaagaa
      481 atcaaacaga ttgctgtaga cggtgcgaaa ttgttacagc gcctgaccga cgaagccggt
      541 gccaacttcc gctttgaata ttcgccggaa agcttcaccg gcacggaacc ggaatacgct
      601 ctggaagtct gcaacgccgt cctcgatgtc tggaaaccga cggctgaccg caagcccatc
      661 atcaacgtgc cgtcgacggt ccagatgtcc atgcctcatg tcttcgcttc gcagatcgcc
      721 tatatgtcgc agaatctgaa ataccgcgac aacgttatct tatcggtcca tccccacaat
      781 gaccgcggta cgggcgtcgc cgatgctgaa atggccatgc tcgccggcgc cgaccgcgtc
      841 gaaggtacgc tgttcggcaa tggcgaacgg acgggcaatg ccgatatcgt cacgatcggc
      901 atgaacatgt atgccttagg cgtcgacccg ggcctggatt tctcgaacat gcccaaactg
      961 gtggaaacgt atgaacgagt gacccgcatg catgtccatc cgcgtcagcc ttatgccggc
     1021 cagctggtct tcgccgcttt ctccggttct catcaggacg ccatcgccaa gggcatgcac
     1081 ttccgcaagg aaaacaacga acagtactgg acctgcccgt acctgtacat cgatccgcat
     1141 gatgtgggcc gcacgtacga tgccgacgtc atccgcatca acagccagtc cggcaagggc
     1201 ggcgtaggtt tcgtcatgga acagaattac ggcatcgaca tgccgaagaa gatgcgcgaa
     1261 gactttagct acttcgtcaa ggaagtatcg gaccacaagc atcaggaatt gaaaccggcc
     1321 gaaatctacg acgtcttcca gaagaactac ctcaatgccg atacgccgct gaaagtcgaa
     1381 gacttctcgc tcaagaagaa aggcgataaa tgggtcggca aagtcctggt ccgcgccaac
     1441 gacgaagaag tcgtcctcga aggggccggc aacggccagc tgaatgccgt cagcaatgcc
     1501 gtctgcaagg cctatggcat cgagttcagc aacctcgtct acagcgaaca cgatttggac
     1561 cgtgactccg attcccgcgg catcgcttac ttcggcctga ccgataaaga aggccatacg
     1621 acctggggcg ccggcgtcga tacggatacc attacggctt ccatcttcgc cttcatgacg
     1681 gccatcaacc gcatggacgg catggctcag cgcgtcaagt tccgggccct caaatccacg
     1741 ccggatacga ttacggcttt caaagccacg tcgggccagc attaaagtat gatgtttgat
     1801 gtgaggggag tgtaattgaa agccactcac atcgaactca agcatgatag aaacttatct
     1861 gtattggatt ttagtgaaat aataaggagt gtatatacat atgggtatga cgatgacgca
     1921 gaaaatcctg gtaaaacatg ccggcgtcga ctcggtgaaa ccgggtgatt taattacttg
     1981 taatctcgat atggtcctgg ccaatgatat tacggcccct ccggccattg cccagttcaa
     2041 caagatcggc aagcccgtct ttgataagac caagatttct ttgatcccgg accacttcac
     2101 gccaaacaaa gacatcaagt ccgccggttt ggccaaaatc gtccgcgact tcgctaagga
     2161 acacggcatt acgcattatt ttgaagtcgg ccgcgtcggc atcgaacacg tcctcctgcc
     2221 ggaacagggc atcgtcgctc ccggtatgct gaccatcggc gccgattccc atacctgtac
     2281 gtatggcgcc ctgggcggtt tctccactgg cgtcggctcg acggacttag gcgtcgccat
     2341 ggctacaggc aaggcctggt tcaaagtccc cgatgccatc aatgtccatc tgacgggctc
     2401 caaacccgat gacattacgg gcaaagacgt catgctgacc ctcatcggta tgatcggcgt
     2461 cgacggcgct ctctaccagt ccctggaatt cacgggcgaa ggcgtcgccg ctctggatat
     2521 gaccgaccgc ttcaccattg ccaacctggc tatcgaagcc ggtgccaaga acggcatctt
     2581 ccctgtcgat gaacagacca tagcctatga aaagggccgc gtccccgatg gctacgacat
     2641 cgtcacggct gacgacgacg cccattacag ccggactgtc gaaatcaact tgtctgaatt
     2701 gaaacccgtc gtcgctttcc cgcacctgcc ggaaaacacc aaggtcatcg gcaccttcgg
     2761 cgacatcaag atcgaccagg tcgtcatcgg ctcctgtacc aatggccgtc tggaagattt
     2821 gaagatcgct gcggacatct tcaaaggcca taaagtccat cctgacgtgc gctgcatcgt
     2881 catccccggc agccagcacg tctacatgga agccatgaag gccggctaca tcgaaacctt
     2941 catcactgcc ggctgtgccg tttcgacgcc gccgtgcggt ccctgcctgg gcggctacat
     3001 gggcatcatg acggctggtg aaaaatgtgt ctccacgacg aaccgccacg tccgcggccg
     3061 caggcgtcac gacgagagcg aactctacct ggctggaccg caggtcgctg cagccagggc
     3121 gagtctcggc aagag
//
LOCUS       DAWCYS010000201         3075 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4760_length_3075_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000201 DAWCYS010000000
VERSION     DAWCYS010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4760_length_3075_cov_8.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          810 a          706 c          683 g          876 t
ORIGIN      
        1 attagagttt ttagacaaaa tgggagaaac tctaattggt ctaattcatc ctgtttgatg
       61 agcctattgt ctcacaaaca gccaggagat taaatccacc gatttatttt ccgcttacga
      121 tactgtcatg atgtagggat agtatcaggg gattttctta ttttctgcgc agatgaatgg
      181 agaaaccgcc tactgtttct tttaagaagg cgactttaat atttcctttt tcgtcgcgat
      241 agcaatggtc ttcatccaaa gcgatcccct gccgttgata gaaggctaag gcgcggggca
      301 tatcgcgtgt atcaatacag atgtggccgt ttgtaccggg catttcatgg tcgcagattt
      361 cggcgatagg tcctgcaaag aaattgtcgg caccgggaat tttgtcctga gccattaatc
      421 ggcacagacc gtcggttacg gcttcagctt ccgttctgcc aggtacatgc agacctacgt
      481 gaccgagtgt gaaatccagc atgtgacgga tgattttttg gcagtgtgct gcgatggcat
      541 cccaatcttt ggccttgact aaatccgaag gggtgatgaa actgccgccg acagctgcga
      601 cattcggtaa atccagatat tcattcatgt tgtcgtaatt tacgccgcct gttggaatga
      661 atttcatggt agcaaaagga ccggcaaggg cttttaaagt tttcggaccg ccatagactc
      721 cggctgggaa gaacttgcaa acttccagtc ctaatccatt ggccgcttcg atgtcggctg
      781 gagatacggt tcccggcatg atatcgatat gattttcgat acaccactgg gtgacctgag
      841 gattataggc cggcgtaatg atgaattcag cacctgcttc tacagcggct tgcgcttgtg
      901 ctacattatg taccgtgcca gctccgacga tgagtccggg aatggtcttc atagcgcaga
      961 tgatgtccag gcaggcatct gtacggaagg taacttcagc aatcgggatt cccccacgga
     1021 ccaaagcttg accaaagggg actgcgtctt gtgcatcttc taagacgatg agtggaacca
     1081 gtccgatttc aaatattttc ttcatattat tgctaatcat ggttgcctcc tatttttcca
     1141 taaacgcttt taactgatcc ggatagggga ggccttcgtt gtctccccgg ctggtaacct
     1201 ggatggcgcc gatagcattg ccgcggcgta cagcttcttt caagggaagt ccttccatca
     1261 atgccgtaat gaccccggct gcgaaaccat cgccggcacc gaccgtatct acgactttat
     1321 cgactttaaa gccagggacg agatattctt cggtagttgt cgttacgtaa gcgccttggc
     1381 tgccgacttt ggtaataacg gctttggctc ccaggttacg atagaaagcg ttgatttttt
     1441 tcggatcatc actgccgatg tgaagtttgc cttcagctgt cccagggaga acgatgtcag
     1501 ctttggccgc catgtcatta atagactgaa tcatgacatc ctgactaggc cagagctgag
     1561 gccgtaaatt agtatcgaag gtaatcagta aacctgcctg acgagcctta tcgaacatta
     1621 agttgacagc agcccgcgtc gattcactga gggctggtaa aatccctgtc aaatggatgt
     1681 ggctgtattc atcgaagcgg attttttcaa catcttcgac ggataacgta gaggcagccg
     1741 agccagcgcg gaaataaaaa atatccggat cgccttggct gacacggcct ttcagcatga
     1801 accctgtctt tttcgtatca ctccaggcaa cgaattcatt gccgattttg ttttcattca
     1861 gtacatggat gattcgctgg ccaaaaggat ctttgcccag tttcgtcatg taggtaacac
     1921 ggtgccccag acgggccaca ccgacagcta cattgaattc tgcgccagca acggccaaat
     1981 catagccact tacactatca agggaacctt cactttgagc aatcaagagg cccatgggtt
     2041 cacctgcaag aattaataca gatttcatag ttcttcatcc tttccaactg atttaacgaa
     2101 ccagataagc tccgtctaca gggatgatgg cgccatttaa ataatctgat gcagtactgg
     2161 caagaaaaac ggccgttcct ttcatatctt ccggtgtacc ccatcgtttt gccggaattc
     2221 ggtctaaaat ttctttactg cgaactggat cttcgattag tttcgtgttc atttctgttg
     2281 ccatatatcc aggcgcaatt gcatttacat tgattccctt accagcccat tcgttagcca
     2341 gtgctttcgt taattgagct acaccacctt tacttgattc ataagctggg acggtgtatc
     2401 caccaaagaa actcagcatg gaagctagat tgattatttt tcctgatttt cgaggtaaca
     2461 tatttcgtcc ggcaagctgg caaagctgaa ataccgtatt taagttgagg tttaacacat
     2521 cattccaatc agttaggggg aattcttcac aaggatgacg acgttgtact cccgcgttat
     2581 tgattaaaat atcgacgcgt ccatttaaaa tattcagcgc ttgttcaaag acggcaggga
     2641 cattttcttg attcattacg tttccgcgta aagcaatcat gtctaaacct tttttttgac
     2701 attctgaggc tgtcttttca acactggcgc tggaaccaat gattaagact tttgccccgg
     2761 cttcggctag tccttcagcc atgccgcgtc ctaagccacg gccaccacca gttacgattg
     2821 catttttccc tgctaaatta aaagcattta atatcgacat aagatacgcc tcctaaaaat
     2881 gataagatga aatatttcac taaatagata ttcatcatat gaatttacaa atgaattaat
     2941 tttattttaa acgataatta tggaaaactc aatagataaa agtgcggaaa ttcgcgttaa
     3001 agcaattatt ttgcagaaac cagctgaaat aattgcgaaa aatgataaaa agttataaaa
     3061 aaaccagggt aatta
//
LOCUS       DAWCYS010000202         3066 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1632_length_3066_cov_4.244437, whole genome shotgun sequence.
ACCESSION   DAWCYS010000202 DAWCYS010000000
VERSION     DAWCYS010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_3066_cov_4.244437"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          643 a          778 c          894 g          751 t
ORIGIN      
        1 tttgccgtac ttcaattcag aagccttggc catgtcgtaa ttgcgttctg cctgggccat
       61 gtcgctcttg acggcgtcga tttccttctt caggccctgc gtgcgcagga tggactgttt
      121 ttctttatcc cactgagctt tgagcttgct ttctttttct ttcagttcgt tcttcgtttc
      181 cgtgatctta gccaggcgtt ctttggaagc gtcgtccgtt tctttgttca gggactgttc
      241 ttcgatgtcc atgtgcatga tctaatggcg cagtgcgtct atcggcgtcg gcatggattc
      301 gatttccgta cgcagtttgg cggcagcttc gtcgacgagg tcgatggcct tatcgggcag
      361 gaaacggtcg gaaatgtagc ggtccgacaa gacggcggca gcgaccaggg ctttatcacg
      421 gatgcggacg ccatgatgga cttcgtagcg ttctttcaga ccacggagaa tggtgatcgt
      481 gtcttcaaca gaaggctgat cgaccatgac cggctggaaa cgacgttcca aagcggcatc
      541 tttttcgatg tatttctggt attcattgag cgtcgtagcg ccgatgcagc gcaggtcacc
      601 gcgggccagc atcggtttca ggatgttgcc tgcatccatg gagccttcag aggcgccggc
      661 accgacgaca gtgtggattt catcgatgaa gaggagaatc tggccgtccg atttggcgat
      721 ttcattgagg accgatttga gtcgttcttc aaattcgcca cggtatttgg caccggcgat
      781 gagggaaccc atatcgaggg agtagagggt cttgttcttc agcgattccg ggacgtcgcc
      841 gctgacgata cggcgggcca ggccttcgac gatagccgtc ttgccgacac ccggttcacc
      901 gatgaggacc gggttgttct tacggcggcg cgagagaatt tcgatggtgc ggcggatttc
      961 gtcgtcacgg ccgatgaccg ggtccaattt attctggcgg gcagcagcgg tcaagtcgcg
     1021 gccgtatttt tccagggcct tgtagttttc ttccgggtta tcgctgttga cgttggcctt
     1081 gcggttggct ttgatggtac tcatgatctt gtcctgggtc aagccaaatt cacggcacaa
     1141 ctgctggact tcgtcgtcgc tttcgctgac gatacccatg aggatatgtt ccgtgctgat
     1201 atattcatcg tgcatggagt cggccaactg ctgagccttg ccgatgacac ggaccatttc
     1261 tgtcgacatg gacaactggc tggagccttt gacagacggg attttcttca acaactgttc
     1321 caagcgggcc tggagcatgg ggacgtcagt gtgacattcc gagaaaatcg tattgagcag
     1381 gccttccggt tctttcgtca agcccatgag cctatggacc gatgagagtt cctggttata
     1441 atggagcgcg gcaatctgct gggcagactg gaaggcttca atgacctttt gcgtatattt
     1501 ttcgtttccc ataattaaga cctccttact tatggtggaa gtaatcattc ttaggagctt
     1561 tcctcaagct ctagtattat tatatcgtca aaagtcaaaa agtcaatagg acaaaaagaa
     1621 aaatagaaaa atatttgaca aaggaaggaa cctttagcta cacttatttt gtcagaaagg
     1681 agacggatat atggacgaaa aagaacggaa agcttttgaa ggcgatgctc aagcgcagtg
     1741 tgacatgggc gatgactatt tttttgaagc tttcgatgcg gatatagaag ataaagatgt
     1801 tttatatagg aaagctatct attggtatca ccgggccgcc cagaagggcc acgccgccag
     1861 tcagtacaac ttggcctatc agtatgaaca cggtctgggg atagagattg acttggaaca
     1921 ggccgtgtac tggtacctcc gggccgccaa tcaaggctac ggcgcagccg agaacaatct
     1981 gggccatctt tacgaaacgg gatgcggcct gccccaggac tatgcccagg ccctgcactg
     2041 gtacggccgg gcggcgcggc acgacgatgc cgacggccag tgcaatacgg ccatcatgta
     2101 ccagttcggc tatgccggca agcgggatta cgaccaggcc gcctattggt atgcccaggc
     2161 cgccgggaag ggccagacca ttgcccagaa caacctggga tacctctatg agacgggccg
     2221 cggcgtcccc caggactggg aactggcggc gcactggtat tacgaagctg ccatggctgg
     2281 tgacggaccg gctcagaata acctgggcgt cctctatcgc gacggccacg gcgtccgcca
     2341 tgacctctgt caggcggcgt attggttcgc ccagtcggct gcgtctggct ataaagaggg
     2401 catgaagaat tatgcccgtg ccctggaaaa gggggaaggc gtggagaagg ataccgtcga
     2461 agcggcctat tggcggcaga aaggaaatga acaggactga catttttacg taattgagca
     2521 tgatccactc ccgctggttc cgcctggtcg tcatcgtcgt gctgatggtg gccggcggcc
     2581 ttttcctctg gcagtataac caggtcgata agccgcagct ggtcaacagc gatggccggg
     2641 tctttgaaaa ggcccgcgtc gtcgatattg tccgcgataa tctgcaggaa gacggaagcc
     2701 gtgtgggcga tcagatcgtc accttgcgca tggaagatgg ccccgatgcc ggacagaccg
     2761 ttcaggcgaa ttgtccgaac ggtcttctct ttgggaccgt ctgccagccc gggatggcgg
     2821 tcatcgtcat ttcggccaac gtcggcgcca tgggtgtcct cttcctcttt ttccccatgg
     2881 tcctgcgcgg tatgggtccc gtcgcagcgg ccttgagcgc agtgacacat aaagtggaac
     2941 ataaatgatt aaaggacgca ggccgccgtt cgcagttcgc aggccgccgc ggattcagat
     3001 gcggcgttcc gccgcgacag actcacgatt ttttgaccta aaatttattc tttgcctgca
     3061 ggccgg
//
LOCUS       DAWCYS010000203         3063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1634_length_3063_cov_6.014960, whole genome shotgun sequence.
ACCESSION   DAWCYS010000203 DAWCYS010000000
VERSION     DAWCYS010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_3063_cov_6.014960"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          758 a          906 c          770 g          629 t
ORIGIN      
        1 tttagtttgc agcaaatggt aaaaaatgta aacccacttc tacaaactcc gtactacaaa
       61 ctataaagga aaagctgcct tgcatgatgg caaaaatctt cgtgcgacag ccccttttta
      121 gttgttcgtg attcgtggtt cgcaggggat gtgcccaaag cttttttggc ccgtcaggtg
      181 ccgaccctcg gaaggcacag gacggttgcc agaacggaca gctgtagcgg gcgtcccggc
      241 tctcctgcca ggagagctgt cagcgcagct gactgagagg ttgcttttga atcgtcgtac
      301 ggttcatggg attcttatag gagagtctgg aaattcaacc tctccggccc gttgggccac
      361 ctcccctatc atgggaggct tggcgccctg caatcggcgg gccgccccta cgaaccccta
      421 gccacgagcc acgaaccact agccactaac cacgaacccc taatctctca gccagcggcg
      481 gatgtcgtct tcggcatctt cgatggtcgt acccaggagg gccaggctct tgccgagttt
      541 ggcatccggg cagagggcgt ggatgtcttc caggctgtgg ccgacgccgc tgccttcgtg
      601 ggtgcagaag ggataaatcg tcttgccgga aaagtcatgg ttttccagga acgtccacaa
      661 ggccatgggc atggtgccgc accagttggg gtagcccaaa aagatgacgt cgtagtcgtc
      721 gatgctgggg atatcctttt ccagggccgg ccgggcattg tcggcctttt ctttcctggc
      781 gatcttgacg acttcctgat agtcattggg atagaccgaa gccgtgcgga tatggaagag
      841 gtcgccgccc gtgaacaggc gcagcatctg ggctgcccgt tccgtattgc ccacgggcag
      901 gtcgacgatc ttgccgctga catagttcat gccggcgtga gaaaaatatg caatgaggca
      961 tttcttacct tgataatcca taagtcaatc aaacctccct gatatgtttg acctaaaaag
     1021 tcccgataca gttttcatta tacggcaaaa atcgccgcca ttcaatcact atcatgaatg
     1081 acggcgattt tattttatca tgcctgccag ctgccctgta tctttccaag gcccatataa
     1141 tgtagtaaaa tagagtttgt caaaagatta ctatgctaag taatacaagg aggtcccttt
     1201 atggctcatg taaatgaaaa ctacttaaaa ctccccggca actatctttt tgcaacgatt
     1261 gcaaaaaaag ttgatgctta ttccaaagca catccggaag ccaatatcat ccgcctcggc
     1321 atcggcgacg tcacccgtcc cctggccccg gctatcatca acgccatgca caaagccgtc
     1381 gacgaaatgg gcaaagctga aaccttccgc ggctacggtc cggaacaggg ctatgatttc
     1441 cttcgccagg ccatcatcga tggcgactac aagacgcgcg gcatcgacct cgaattagac
     1501 gaagtcttcg tcggtgacgg cgccaagacc gatgtcgcct gcatccagga aatcttcggc
     1561 gacgacctca aattcgccgt agccgatccg gtctacccgg tctacctcga ctcgaacgtc
     1621 atgttcggcc acacaggcga ctggaatgcc gaaaaaggca tctacgacgg cgtcgtctat
     1681 ctcccgtgca ccccggaaaa cggcttcaaa gccgaaccgc cgaaggaaaa agtcgacatc
     1741 gtctacctct gcaacccgtc caacccgact ggcacggcca tgagcaaaga agaattgacc
     1801 aactgggtca aagctgccaa agaaaacaac ttcatcatca tctatgattc ggcttatgaa
     1861 acgtacatca ccgaaccgga cgttccccac agcatctttg aaatcgaagg cgctaaagaa
     1921 gtagccatcg aactccgctc ctactccaag tgcgccggct tcacgggtac gcgctgctcc
     1981 tatgtcgtcg tcccccatgc ctgcgtcgct tacaagaaag acggcacggc tgtcgaactc
     2041 aatcccttgt ggaaccgccg tcagtgcacc ttcttcaacg gcacgccgta catcgtccag
     2101 cgcgcagctg aagcttacta cagcccggaa ggccagaaac agtgcctggc tgacgtcgaa
     2161 tactacatgg aaaacgccca catcatccgc gacggcctga ccgaagccgg cttcaccgtc
     2221 tacggcgcta ccaactcgcc gtacgcctgg gtacagacgc cgaacggcat gaagagctgg
     2281 gatttcttcg acctgctcct ggaaaaagcc cacgtcgtca cgacaccggg ttcgggcttt
     2341 ggcccccacg gcgaaggcta tctccgcctg accgctttcg gcaccaaaga aaacaccgtc
     2401 gaagctgtca aacgcattgc ggatttaaaa ttattcaaat aaaggtggtt taaccattga
     2461 gtaccaatct ggaagtaggc atcgtcggcc tgccgaatgt cggtaagagc acgctgttca
     2521 acgcgattac caaggccggc gcagaagcgg caaactatcc cttctgcacg attgaaccga
     2581 atgtcggtgt cgtagaagtc ccggataaac gcatccaggt cctgaccgac atgtacaaac
     2641 cgaaaaaaac aattcccgct gtcatgcgct tcgtcgatat cgccggcctc gtcgccggtg
     2701 cctccaaggg cgaaggcctg ggcaacaagt tcctcagcca catccgtgaa accgatgcca
     2761 tcgctgaagt cgtccgctgc tttgaagact cgaacatcac ccacgtcgac ggctccatcg
     2821 acccgatccg cgacatcgaa acgatcaata cggaactgtg cctggccgac ctggaatcgg
     2881 cccagaaacg ggcggaccgc atcgccaaag tcgctcagtg tggcgacaag gacgccaaag
     2941 tcgaatacgc ggtcctgaaa aaagtcctgg cctgcctgga aaacggcgac ccggcccgca
     3001 aagccggcct gacaaaagat gaactgccgc aggtcaaaga actcaacctc ctgaccttga
     3061 agc
//
LOCUS       DAWCYS010000204         3002 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1678_length_3002_cov_5.079742, whole genome shotgun sequence.
ACCESSION   DAWCYS010000204 DAWCYS010000000
VERSION     DAWCYS010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1678_length_3002_cov_5.079742"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          605 a          836 c          888 g          673 t
ORIGIN      
        1 ggttatgagt cagctggaaa gccgagtttt ctgtcgaact gcccatggcg aattcgtcca
       61 tattggtctt gcccaggctg acataatcag cagccatcaa tttttcgatg accgtcgcat
      121 tgtacggcga tttgaaattt tccagcatct tcgatgcaca cgtagccttc tgacctttga
      181 tgcagatgtt gtccttgatg gcaccgggaa tcccggcgag gtcactgatg gcttcaccgg
      241 ctgcgatttt ggcatcgaca gcggcagccg cttccagggc atagtcgcgg ttatccgata
      301 aaaacgcctg tactttcggt tctactgtat ctttatgtgc aagaaaagcc ttggtcaatt
      361 cgacagccga tacgtcgccg ttgaccagtt tttcatgcag ttcatggatg gttaacatac
      421 atacgcctcc ctgttaatcc tggatgactt tcggaacttt gaaatagcca tcagcctgtt
      481 ccggcgcatt ctgaagcgct ttttcattgg ccagtgacgg ctggggaacg tcgtcgcgca
      541 tgacgttgga catggatacg gcatgggccg tcggcttgac atcggtcaca tcgacttggc
      601 tcaagagatc catgtacgat aaaatgtcgt tcagctgttt cccgaccgtt tccatctggt
      661 cttccttgac ttccaggcgg gacaacagtg cgatcttctt tatttcttcg gcactgattt
      721 tcatactgac actccttgca gatattttta gcgtgacaaa tcggaatcat ttgtcaaaat
      781 tcaacacata tataatttaa tcaccttttg ccatattttg caagcctcat cgcctgtatg
      841 cgataccgca ggggccgtcc cagagccttt ctgccccatc cgatgcagga cgattgcccg
      901 aacggatagc agcggcgggc ggcccgctac gtgctgaggg cttccccaag catcgttgtt
      961 actttgagtt tgtcgcggcg gaatgccgca tctgagtccg aggaaaaagc cgtggctcgt
     1021 ggctcgtggt tcgtggctcg taaaaatcca ccattatacc agaatgggct atgatggaat
     1081 ctcacagcgg gctgccctgc gggacgccca gagggcgtcc cacctattct ctcgtcaggc
     1141 ggtagatgag ggcattgcag atggcggcgg cgatggtgct gccccctttt cggcctttgg
     1201 cgacgatgta gggaatgccg gcttccatga tgcattcttt ggcatagacg acgttgacga
     1261 aaccgacagg gacgccgatg atcagggccg gccgcagctt cccggcatcg atgagttccc
     1321 gcaggcgcag gagggctgtc ggggcattgc cgacggcgac gatgaccggc ccttcgaggg
     1381 ctgcggcttt atccatggaa gcctgggccc gcgtcgtccc ctgcgcttta gcagcggcag
     1441 ctacgtcttc atcggccatg aagcagactt tgcggcagcc cagttttttt agacccggct
     1501 ggctgatacc catgcaggcc atgttggtat cggtcacgat ggtgcagccg cctttcaggg
     1561 ccgtcaaggc ctgatcgacg gcgccagggc tgaaacgcag gttgcggaca tagtcaaagt
     1621 cggcactggt gtggacgacg cgcttgatga tatccttttc gtctggcggc aggatgatgt
     1681 cgcctaattc gtcttcaatg atttccatgc tgcgccgttc gatatcggct ggtaaaatgt
     1741 gttcaatttt catgagttcc ttcctttcga tggatgcagg ctgcgacgaa gcgggcggcc
     1801 cagtcccttt tccccaggaa atggatgtgg ggatagccgc cgatgaagtg gtcgttcgcc
     1861 atgatgcagg gccaatggcg ctggctcccg gccttataag ccgtaaaatc atggccgttg
     1921 tccgtgctgt ctgaatagtg gaattcgtgg gccgtcacgg tgtcgccagc gcagcagagc
     1981 agattgtctt tttcagcctt cagggtgacg taaccgaaac gcttcaggga cgaggtcatg
     2041 aaggtttcgc caggtacggc gccgacccag ggccacaggc ggtcctggct gtcgcgaaag
     2101 gcctgatgga gatacatgaa tcccccacat tcggcgatgc acggcagccg ggcgtccaag
     2161 gcacggcgaa gggatgcacg catcgacgcg ttctggctca aggcctgggc atacagttcg
     2221 ggatagccgc cgccgagata gagaccgtca caaggcggca gttccccgtc gtggagggga
     2281 ctgaaaaaga ccaggtccgc gccggcctgc tgcaggacct ggaggctgtc ttcataatag
     2341 aaacagaagg cttcgtcccg ggcgatggcc aggacggccc ggccaggatc gggatgaaag
     2401 gccggactgc cagacccggt caaaggcgct gcctggcggg cacaagccag gagttcatca
     2461 atggcgatag tcttttcggc ctgggcggcc aaagcggcca ttttcccttg gaggtcagta
     2521 atttcatcgg ctgtgaccag tcccagatgg cggctggaaa aagtgcagtc cggcatatcc
     2581 gggaagcagc ccagggcttt caggcccgat gccttttccc aggccccttt gaaatgctca
     2641 tagaccatgg gcttgatgcg gttgacgatg aagccgacga cgtggctgtc agcccggaca
     2701 gcagccattc cctgcaagac ggctgccagc gacaggccgg caccgcggcc gtcgacgacg
     2761 aggacgcccg gcgtatctgt cgctgcggcc aattcatagg cgctggcctc cgaacccgtg
     2821 ccgacgccgt cgtagtagcc catggcccct tccaaaatgg ctacatcagc cgcagcacca
     2881 tggcacagca gcgtctcccg ggcagctgcg gcgcccggct cgtggcggcc gaggagaaag
     2941 aagtccagat tatacgacgg cgtatccagg acccggcggt ggaacatggg gtcgatataa
     3001 tc
//
LOCUS       DAWCYS010000205         2988 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1690_length_2988_cov_4.592908, whole genome shotgun sequence.
ACCESSION   DAWCYS010000205 DAWCYS010000000
VERSION     DAWCYS010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1690_length_2988_cov_4.592908"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          612 a          891 c          787 g          698 t
ORIGIN      
        1 ccggtgcaca agcttgtctg cgcctcgaac gccaacaacg tcctgacgga cttcctgcgc
       61 acgggcgtct acgaccgcca gcgcgacttc ttcaagacga tctcgccgtc gatggacatt
      121 ctgatctcga gcaacctcga gcgcctgctc taccacatgg cggaggacaa ggcgaaggtc
      181 gcctcctgga tgcagcagct ccagcaggag ggccgctacg acatcggcag cgagctgctc
      241 gcgaagatcc agcagacctt ctgggccgac tggaccgcgg atgcggacac ggaagcgtgc
      301 attgccgacg tctatgagaa ggaccattat gttgccgacc cgcacacggc cgtcgcttac
      361 ggcgtcggca tgaaccgcca tcgccagggc ctcgacgaag gccgccacac ggtcatcatg
      421 gcaacggctc atccctacaa tttcccgccg gtcatcttcg aagccctggc cctgcgtcag
      481 agcgatgatc cctttgacat gctggccgac atcagcagcg tcacgggcat cgccctgccc
      541 aagcctttgg cggacctcaa ggataagccc atccgcttca gcgactccat cgacaaggaa
      601 gacatgaaga aagctgtctt gtccttcgtc gacgccattg cggccaaaca ctaggaaaga
      661 ggctttgatt tgagtagagg tgtcatttac gcaatcttat cggctttggc cttcagtatc
      721 cagaatgtca tcgtaaaaga attatcctat acgacgagca ctggggaaat cgcgttcttc
      781 cgcggtttcc tcagtgccct tctgatatta gggctcatgc gcatccaaca cgtacatctg
      841 tcgaagcggg atataccgac gctgaccttg cgcggcgtcc tcggcggtat cggcatgatc
      901 tgcattttct atgccctgcg cgggatgccc ctggccgatg tatccatcat ttcccagttg
      961 tcggcgttct tcgtcatgct cttcgcggcg attttcctca aagaagtcct gccgcctggg
     1021 tcgaagctgc ccctcctggt catcttcatc ggcggctgcc tcgtcgtccg gccctggaac
     1081 ttttcgtcat tcaacgtcta ttccctgttc gccctggggc aggccgtctt tgcggccggt
     1141 gcctatacga cagtcagcaa gctcactggg tctggacgcc accatcctta tgaagtggtc
     1201 ctgtactttt tggtctgtgc agctctgtcg ggcatcgtcc tcatggccct gactgatggc
     1261 tttgtcatgc cggctcacgg cgactggatc tattacggcg gcctggccct gttcagcgtc
     1321 atcgcccaga tttggatgac caatgcctat gcaacggcca acccggttgt cgtcagcttt
     1381 gtttcttata taggcgtctt cttcaacgcc ctttggggct tcgtcatctt tggcgaaatc
     1441 ctcaccggct tgaccgtcgc cggcggtgta ctcattatcg gcggttccat gtacctgacc
     1501 aagctcaagc acgataagat tgctgaaatg gccaagatgc aggaacggaa gcagaaagag
     1561 gcataacatg actacgaaac agattcatga aacacacatt aaaatcgcct tttttgccat
     1621 gctgctcatc atgctggctg ctatctatgt ggccatcccg gacttttaca gcacggtctg
     1681 gcagctgtcg acgagcggca atatgcaggg gactatcgac tacctgcgct ctttcggcgc
     1741 ctgggccgtc ctggtcagta tgatcatcga catcgtcatc aatatcgtcg gctttttgcc
     1801 gtctatcttc atttccacgg ccaatggcgt ccttttcggc gttgccggtg ggactgtcgt
     1861 ttcctgggta gccgaaacaa tcggcgtcat catcagcttt ttcttcatgc ggactttgtt
     1921 ccgcaacaag gccaagaagg tcatagaaaa gagcaagatg ctgagcaagc tcgaccatta
     1981 cagtacctgg caggccatga ccctggcccg ggccgtgccg tattcgccga acggcctggt
     2041 cacggccctg ggggcgctga gccgcatcag ttatcgcgat tatatcctgg gctgcttcat
     2101 cggcaaattg ccgtctgtcg cctgtgaggt cctggtcggt cacgacctgg tcatgatgga
     2161 agaacattcc ctgcgcctga ctatcgtcct catcgtcatc gccatcgtct atggcggcct
     2221 gtggtactgg catcgccgtc agcaggccaa gaaagtcctc cacaagattg ccgacgacct
     2281 caaagacggc gaaggccgca gttaatttca gaaaggataa agtatcatgg aaaaagaata
     2341 taaattggca gaacaatacc atttgcaggg caataactgc attgaatcgg tcctcaagac
     2401 ctgtaacgac acgatgaacc tccagctgcc ggacgtcgct atccgcatgg cttccggcat
     2461 gggcggcggc atcggccggt cgggctgtgt ctgtggggcc ttgagcggtg cctgtctggt
     2521 catcggcgcc ctcgtcggcc ggctcacacc cgatgaaaag cccttgcccg aagtctacaa
     2581 atacacccgc gagttccatg accgcttcaa agaacacttc ggcgccacct gctgccgggc
     2641 cctccacacc ctcagtgtcg gaccgccgga ataccgtaag cactgcatca agattacggc
     2701 cactgccgcc gacatggtct ccgacttcgt cgccgaaaaa ggcttgttga agtgaggtta
     2761 gtggctcgtg gttcgtataa caaaaaaagc tgtcgcttat gcgacagctt tttttcgtct
     2821 gtaggggctc gcacgtggcg agcccgtttc aggatatgtt cgatttttcc gacataataa
     2881 agcgttggaa ccatttttca ggagcgggcg ccccacgtgg cgcccctacg catttttggc
     2941 gaatcactag ccactagttg ctaactgtta actccatctc ttacaaga
//
LOCUS       DAWCYS010000206         2963 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1706_length_2963_cov_6.048143, whole genome shotgun sequence.
ACCESSION   DAWCYS010000206 DAWCYS010000000
VERSION     DAWCYS010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_2963_cov_6.048143"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          584 a          736 c          624 g         1019 t
ORIGIN      
        1 tcgtctatgt gtttcgtgct aatgtccgtt tttctcacct ctttttctaa attggagcgc
       61 taacattgct aaacgtcgct catcttcggc ggtcattatt cgctggctgg ccttgctcat
      121 tacttcttcg tgtacgccaa aaaagaattc cttcattttc ttcttgtcgt cctggacgta
      181 ttccgggatg tgctccagca ccgtgatagc tagtgttttc gcgccgacat atttcccgta
      241 gtccagtacg gtgtagtcgt ctttatggcc gttccgggca gcttcattgc tccgtaagtc
      301 agtgatgcag tagtccagca agaccttcct tatgtcgctg atcatctttt cttcatccat
      361 ggttacacct ctttcttttc cagtaattcc gggttttcgt acgcgttgcc gatgactttg
      421 atttgcccgt cgtcgtcaat aagcatgtct aagtcaaccg tgacgaacgg ataggggtca
      481 tctcctatgc aacgtgccag taatactccc atttcgtcgt ctatgacaaa ccgtttcttc
      541 atgtctttgc gggtggcttc gacaatatcg ccttcgtaga tcattacgtc atcatagcta
      601 tcatcgtagt aagtcgtcca tggcatgagg accacttcgt caaatcttgc gacgatgact
      661 tcatctttag ctcttccttt tactctttct ttgcggaagt cgatttcaga tacatgcatc
      721 atcaccttct tatcgtggtg ccatgcccgg tagtcaatcc attgcggttc catatcattc
      781 tcctatcttt cttgcgttgg cggttcttac tagttcctta atcaatcgtt cgccttcctc
      841 ttgggtgtta aagtgtaagc ttacttttac tttgctttcg aggacaatag tcagccaata
      901 ttgggcgtat tctggattcg gcggcaggct gatggctatg attttttccg gattgatata
      961 agtcctatca cctatcttta ttagtttcat gctctccctt cctttctgct tgcccgtctt
     1021 cattcacgta tatttttatc ttagctatga ctctttcgct ttctttgagt acgcggacga
     1081 gatacagtgt gacaagcccc attgtcccga ttcccacggc aatcagacta tgtgccagac
     1141 agttcgctgt cagcagtaac gcgatagcca atgcatccat caagacaacg tagacagtca
     1201 gtatattgtt tagcttcatg ttattccttt ctagccttag ttaatcgttt tagattccct
     1261 tctacgagct ttttctttaa cttcgggtgt agttcataga gtggaaattt taagtccgtt
     1321 atttcgctta cagtgatttc cgcgcgcggg tcttcgacgt cgatgccggc tatcatcgag
     1381 ccgtcgattt gagctatgta gccgtcgtcc tcgatgattc ccgcgctttc aagtatatct
     1441 gccgtcgcct ggaccagccc gaataaatcg ggccagccct tgcggttcgg catataatac
     1501 ctagcggtca tcgtgacggc gcatccaatc ggcttaatag actttttttg ggcttttaac
     1561 ttccataaag catcctttgc atattctcta taggctttac cctgtacaag gccgtagcgc
     1621 gtcttctgga gcgaattttt cttagtcacg gggcgcccgt ggatgacgaa tttgtatgtc
     1681 atttcttttc cccgaaagtg tggagcaata agtcgacgag gtgttgttcc gagtttgcga
     1741 tgattttatc gtcttcatcg tccatgccgt ccggatcttc ttccggttct tccgggtcga
     1801 ggtcgataat gtgttccgtc gtcaccttga acggttcgtt gttgagtgcg gactttgcta
     1861 cgatgagcat cacgtctgcc aagtgtgtac gtatcgtaat gtctttgtaa ttaaagcctt
     1921 ctatcgtaat ggagaatttc tggtccgggc tactgatacc caggatagtc gccaatgtgg
     1981 cttcgaggtt gattctttct gctttagtca tggttttcat gctccttttc gcttgtaatc
     2041 aaaaaatcta aatactgccg ggccttcatg aggtctttta gtggtgtccc tttcgccggg
     2101 tagcggtaca ggtacttgac gatattgccg atgtacattg catcagctcc cgtcgccccg
     2161 ctggtcatga tttcaatggc cttcgtacat tccgtaccgc gccatgtgta atggttcggg
     2221 tgttgtacgt cattcatctg catcagccac tttcatgaat cgccatttcc atgcaacgtc
     2281 cggatatttt tcgtggttta cttcgctcat gaacatgcgt agcggccgcg cccagatgag
     2341 tttaggtttt tgggtatcct ggtacaccac gtccagcccg tctctctctg tatctcctgc
     2401 aatgcagatg atttcataga tatggccttt gaagtgtttc catttttcgc ccggcctcgg
     2461 ataatcgtgc atgaaaaaaa gttccatgtt attcatctcc ttcatcccgt aacgtcttca
     2521 acagttccgt agctctttca atacgtttcc tgatggattc tttattggca tgcgcctctt
     2581 tacgtttcct gaagcagtta ccgactgcga tattcaaaat atctgtgttc gtattgtgat
     2641 tgatggtgct atagatgcgg ttctcatcgc cagcataata ataaacttcg ttttcttccg
     2701 gaacgaacag cttgttcagc ttttcaacta tcctttgcac cgccagtttt atccctattt
     2761 ccgggttaaa tgcgtcattc gggtggcatt tcgcttttcc cttgtaaatc gtttcgccgt
     2821 cttctctttt gtaccatact ttgatagttc cgttcggata acatttaacc ttatctaatc
     2881 cgcctataag attaagtcct actttttcgg cttctttttc ggcaacacgt tccatgtagc
     2941 tcgaaaaaat ggggctatca tag
//
LOCUS       DAWCYS010000207         2950 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1715_length_2950_cov_5.377893, whole genome shotgun sequence.
ACCESSION   DAWCYS010000207 DAWCYS010000000
VERSION     DAWCYS010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_2950_cov_5.377893"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          795 a          835 c          751 g          569 t
ORIGIN      
        1 cttccaggct ttggaaaaat tcaactctga agtcctgaag acggctgtca acaatgcaga
       61 agtcgatatc ccgcaggtca tgatcgacga caaaatcgac cagatgatcg aagaactggc
      121 catgaaactg gaaacacagc gcatgagcct cgacgattat ctcaaataca tgaaccagga
      181 tatggacaaa ctcaaagaac attacgctga accggctaaa gaaaacgtaa agatggacct
      241 cgtcctcgaa gctatcgcca aagctgaaaa catcgaagtc aaagacatcg atctccaggc
      301 tgaaatcatc acgatggctc agaacttcgg tgccgatccg aaagaagtct acaagatcat
      361 tttgaaagaa catcgcgttc ccatgctcgt acagtctgtc ggccgtaaaa aagcagccag
      421 cttcatcctg aaaaatgccg tcgacccgaa cgaagacaaa aaagaagaag ctaaagctga
      481 agacgcaaag gctgaagact aatcccggaa acgcattctc cggaggtgtt agttttgaca
      541 aatatgtatg tacccatcgt cgtcgaacag acggcacaag gggaacggag ctatgatatc
      601 tattcccgcc tgttgaaaga ccgcatcgtc ttcctcggcg gcccgattga tgataccgtt
      661 gccaacagca tcatcgccca gctccttttc ctggaagcag aaaatcctga caaggatatc
      721 cacttatata tcaacagtcc tggcggcgtc gtcacggcag gcatggccat ctacgatacc
      781 atgcagtaca tcaagccgga cgtctcgacg atctgcgtcg gttccgctgc cagcatggcg
      841 tcggtactcc taacggccgg tgccaagggc aaacgcttcg ccctgccgca ttcccaggtc
      901 atgatccacc agcctctcgg cggtgtccag ggccaggcta cggaaatcga aatccatgcc
      961 cgcgaaatcc tgcgcatgcg caaggaattg aacggtatct tgtctgaaca cacaggacaa
     1021 cccattgacg tcatccagaa ggatacggaa cgggataatt tcatgactgc cgaagaagcc
     1081 aaggaatacg gcctcatcga tgaaatcctg acccgtccgc tgccggatga aacgaaatag
     1141 gacaggagga agacaagatg aacgatagga atgatgaacc gcgttgcagt ttctgcggcc
     1201 ggccccagag cgaagtacag aagctcattg ccggcccggg tgtctatatc tgcgacgaat
     1261 gtgttgccgt agcgcagcag atcagggaag aagaaatggc gctagaaacg ccggacttcc
     1321 agctcaagga cctgccgacg ccgcatgaaa tcaaggctac ccttgatgaa tacgtcatcg
     1381 gccaggaagc agccaagaag accctttccg ttgcagtgta caaccactac acccggctcc
     1441 agaccaattt cagtgcctct gatgatgatg tagagctcca gaagtcgaat atcgtcatgg
     1501 tcggcccgac tgggagcggc aagaccctcc tggcccagac cctggcccgc ctcctcgatg
     1561 tgccctttgc catcgccgat gcgacgagcc tgaccgaagc cggctatgtc ggcgaagacg
     1621 tcgaaaactg cctgctcaag ctcatccagg ccgctgatta cgatattgcc aaagctgaac
     1681 ggggcatcat ctacatcgac gaaatcgata agattgcccg caaatcggaa aacccgtcca
     1741 ttacccgtga cgtttccggt gaaggcgtcc agcaggcctt gttgaagatc ctcgaaggga
     1801 ccgttgccgg cgttccgcct cagggcggcc gcaagcatcc ccaccaggaa atgctccaga
     1861 tcgacacgac gaacatcctg ttcatctgcg gcggcgcttt cgatggtatc gacaagacca
     1921 tcctcaaccg tacgacggac aagaatatgg gtttcggtgc ggacatccac tccaagtccg
     1981 aacactccat ggaatcgctc ctcaaggaaa tcatcccgac ggacctccag aagttcggcc
     2041 tcatcccgga attgatcggc cgcctgcccg tattggttac tctcaatcct ctcgatgaag
     2101 atgcgatggt acacatcctg acagaaccga agaacgccct ggtcaaacag tatcagaaga
     2161 tgctgtccat ggacgacgta gatctcgaat ttacgccgga agctctccag gccattgcca
     2221 aggaagcgct gcggcgcaat accggtgccc gcgggcttcg ctccatcatc gaacggatca
     2281 tgctcgatgt catgtttgat gtaccgagcc gcgacgacgt agccaagtgc atcgtcacgg
     2341 aaaaatgcgt caaagaaggc gctgagcccg acctcgtatt gaaagaggca aaatgatata
     2401 atgtacaata aaactcattt cttaaaccgg aaatgagttt tatttttcca tagaagaagg
     2461 tgaagtgtat gaacgaaaac gttgtgctca acgagcaaga tatcctggcc ctgccttttg
     2521 tcccgctccg gggcatcgtc gtctatccga agctggtcag ccacatcgac atcggccgcg
     2581 atacgtccct ggctgccgtc gactacgcca tggaacacga ccggcagctc ctggtagcga
     2641 cccaggtcga tgaaaacgaa gacaatccgg gttttgacga tgtctatacc atcggctgcc
     2701 tggtcaagat tgaacagctc ctgcgcatgc ctggcggcct ggtccgcatc ctggtcaacg
     2761 gcatttcccg cgtccgcatg cagggctttt cccagaaagc cgattacctc gaaggggctg
     2821 ccgtcaaagt ggctgaaatc agccgcaacg aagtcgaaga agaagccctg cgccgggtcg
     2881 tcctcaagcg gctcctggac tggatgggca atctccgcga cggcgaagaa gccagcgaaa
     2941 atgccaagaa
//
LOCUS       DAWCYS010000208         2933 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1730_length_2933_cov_5.145587, whole genome shotgun sequence.
ACCESSION   DAWCYS010000208 DAWCYS010000000
VERSION     DAWCYS010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1730_length_2933_cov_5.145587"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          615 a          859 c          843 g          616 t
ORIGIN      
        1 cttgtttact ggcttcatgg gcattttttt atgaattaag tgtcaaactc ccggggcttc
       61 ctgtaggaag cccgccagag agattcgacc acagctgatt cgagtataat ggcaggcttt
      121 tacgagccac gagccactat tttttcacga gacgagggtc actctaggcg gcctgcgacc
      181 ggcggggccg ataggctccc caacacatgc aaaaggcact ggctaggtct gcaccgatgt
      241 tgtataatag agactggatt tgtaaagaaa ggatagaaca tacatgatta cacgcaacca
      301 gatcattcat tatacatcgg atgaactcat ccgggtatct attgccttga cgacgggtat
      361 cgtcgagaag gcacggcagc gccataattt atcgcctgtg gctagtgcgg ctctgggccg
      421 gacgatgacc ggtgcagccc tgttggctgg cgattataag aaccacgaag gcgtttccct
      481 gaaggtcgcc ggtgacgggc ccttaggcgc cgtccatgca gactgctttg atacgaataa
      541 gctccgcggc tatgtagaca atccgcaggt cgacctgccg ctgaaggtca atggcaagct
      601 cgatgtggca gccggtgtcg gccagcacgg ccagttgacg gtgacgcgtt tcacgccgga
      661 aaagacgact tatacgagcc aggccgattt ggtgtccggc gaaagcgccg aagacatcgc
      721 ttactatctg ttcactgcgg aacaggtgcc gtcgacgatc agcctgggcg tcctggtcag
      781 ccaggattac accattgctg cagccggcgg tttcctcgtc cagaccctgc cgggcgcgaa
      841 ggatgaagac ctggcccgtg tcgaagccaa tatcacccag gtcggtgctt tgacgacgta
      901 tttgcaggac catcccaatg gtgaaggcct cattgacatc atcctcagcg gtatgcattt
      961 caaggaactc taccggcaga atctgtactg gcgctgcacg tgctcgcgcc agcgctttga
     1021 aaatgctctg ttgtccctgc gccaggaaga taaggcagcc ctcttgaagg acccgcaggt
     1081 ggatatgccc tgtcagtatt gcggggccat atacgtattg tctcacgatg aactggcagc
     1141 gctgtatgaa gcggctgccc agcgccataa gaagaattga ttctccgaag gaggttttac
     1201 gatgggactc attgccatca tcggcggtac cggtgtctgc gacccgaaga ttttgtccaa
     1261 tgtccatgaa gactatatgg atacctttta cgggaccatt cattattggc aggggacgta
     1321 taaggataag gaactcgtct ttttgccccg ccacggccgg aatcattcca ttccgccgca
     1381 cctcatcaat taccgggcca atatcctggg cttgaagcgc ctgggcgtca cggccatctt
     1441 gtcgacgacg gctgtcggct cgctgaaccg cgatgacaag ccgggcgatt tcgtcctgcc
     1501 cgaccagttc ctggatttca ccaaatgccg tcatacgtcg ttcttcgacg gtggcgaaaa
     1561 cggcgtcgtc cacgtggaca tgaccgaacc gtactgcccg accctgcgcc ggaccgtagt
     1621 ggctgcggct caggcccttg gcggttatac gctccatgat aagggaacct atgtctgcac
     1681 ggaagggccc cgcttcgaga cgccggctga aatcaggatg tatgaaaagc tcggcggcga
     1741 tttggtcggc atgaccaacg tccccgaagt ctgcctggcc agagaagcgg aaatctgcta
     1801 tgccacgatt tccatggtca ccaattttgc cgccggcatt tcgccgacgc ccctgaccca
     1861 tgaagaggtc gtcgaagcta tggccgagaa ttcccagcgc ctgcagcagc tcatcctgaa
     1921 gagcatcgaa ttatatgata atgaggactg cgcctgccgc cacaccctgc aggcttatgg
     1981 agggtttacg gtataatgga tacactgcgc tggcaagcgg gcacgctgtt cctcctggac
     2041 cagacgaagc tgccctttga agaaatctat ctgccctgcc attcctggca ggacgtgcgc
     2101 caggccattt ccgtcctggc tgtccgcggg gcgccggcta tcggcgtcgc tgcggcctat
     2161 gccgtcgtcc tggcggcgcg taccctgtgc cagaaagggg gagatacgcc ctttttgacg
     2221 gacctggaaa ctatctgccg ggaccttgat gcggcccggc cgacggcggt caacctgcac
     2281 tgggccctga cccagatgat ggcttgtgcc cgccagcatg aagccgacgg cgctgaagcc
     2341 acgacggcag ccctggaaga aaaggccgtg gccatccata aagccgatat tgccatcaat
     2401 acggctatgg cccggttcgg ggccgacgag ctggaaaaaa tcagccgtcc tttgacgctg
     2461 ctcacgcact gcaatgccgg tgccctggct acggctggtg tcggcacggc gctgggtgtc
     2521 atccgcgagc tccatcgccg gggccaggtg tccatggtct acgccgatga aacgcggccc
     2581 ctcctgcagg gcgcccgcct gacggctttc gagctcatgg ctgaccatat cccggtgacg
     2641 ctcattaccg acaacatggc ggcctggacg ctcaagacga aaggcgtcga tgccatcatc
     2701 gtcggtgccg accgcatcgc cgccaatggc gatacggcca ataagatcgg gacttacggc
     2761 ctgtccctga tggcaaggga actggatatt cccctgtaca tcgccgcgcc ggcttcgact
     2821 ttcgatttgt ccattgccga cggcagccag atccctattg aagaacggaa tcacgatgaa
     2881 gtccgttctt tccggggtgc ggcggcagcc ctccccgaaa cgcccgtctt caa
//
LOCUS       DAWCYS010000209         2933 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1729_length_2933_cov_6.352328, whole genome shotgun sequence.
ACCESSION   DAWCYS010000209 DAWCYS010000000
VERSION     DAWCYS010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_2933_cov_6.352328"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          586 a          836 c          777 g          734 t
ORIGIN      
        1 cggcgacatc ctggtcatcc acgacacagg cgcacacggc cattccatgg gcttaaacta
       61 aaacggcaag ctcagttcgg ctgaactcct gctccatgct gatggcagcg tcacccagat
      121 ccgccgggct gaaacactgg acgacctctt cgctaccctt aatttctctg atttataatc
      181 gaatatgaat gaagggcatc tcatgaatta aaaattttca tgggataccc ttttttattg
      241 taaaattttt ttgaatttta atctcctttt aatctttacc ctatatactg aacatacatc
      301 atctcatcgt tcaaagagga acagaaaggg ggagccgacc gtgatgagcg cctatgatat
      361 cctgacgggg atacaactct gcatggctat ggccatgatc gcctatggca tttacttccg
      421 gaagagccgc atgtgccgcc tgtggtggtt cgcaggcccc tgcgtcctgg ttgccatctg
      481 ggcagcggct gtattatatt attatacgga atatcctttt ttgacgaccc tctgtttcct
      541 catggccttt ttcctgggcc tgggtctggg cctgtggttc aagaagggcc tgccggaact
      601 gacctattgc cgcaagcagc acagccttcg ttgtccgccc ttgcggacgg ccctgccggt
      661 gaatctcttt ttctgcctga ttttcgcctc gttccaggcc gtggcctacc acatgccctt
      721 catcacccat tcgtggctgt tcaacgaggt cctgggcttt gcgccgggca tcggcatggg
      781 ctggctgtgg ggacggggat tggccatgct gttcgatatt cccacgcggg gacagcaggt
      841 ctataaagat atttaggagg gactttgttt atggatagta gtgcacggag agaaaagatt
      901 cttcagattc ttcaagaatc gtcgtcggcc gtcaccggga cggccttatc gaaagcctgc
      961 aacgtcagcc gacagatcat cgtcggcgac gtggctattt tgcgcgccca ggggattgcc
     1021 atcatctcga cgccccgggg ctatcagctg gtcacaccgg ccgcaccggg ctgtacgcgg
     1081 gtcttcgtct gctgccatgg cagcgatttg atggaagcag agctcaatgc catcgtcgat
     1141 aatggcggca tcgtccacaa cgtcgtcatc gaacacgacg tctatggcaa cctggaaggg
     1201 accttgaacc ttcattcccg ccgggatatc cagcagtata tcaagcgcat gcatgattcc
     1261 catgcggaaa tgctcagtac catcagcggc ggcatccata cgcatctcgt cgaagcggcg
     1321 acggaagaag aactcgatgc catcggccag gccctgcgcg acgtcggcgt cttatataag
     1381 gattgacatt tcccctctat atcggtacaa taagaccatg taaatataac tgtcatgaca
     1441 tatgtacaga taggaggaac tgttgttatg gtattagaac gactggaagc gctgcccctg
     1501 ggccgtttcc actataaact gctgctggtc acgggccttg gctggctctt cgactcgatg
     1561 gatacagggc tcatcgcctt tatcctgcct gtactggcca aagaatgggg cctggcaccg
     1621 gggcagatgg gcctcatcgg cagcatcggc ctcatcggca tggccctggg cgccgtcgtg
     1681 tccggcacca tcgccgaccg cataggccgc aagaaggtct tcaccattac ggtcttattg
     1741 tacagcattg cttcggcctt ctgcgccttg tcctggaatt atcaatccct gctcgtcttc
     1801 cgcttcctcg tcggcttcgg cctgggtgga gaactgcccg tagcggcgac gctcgtttcc
     1861 gaatatgcgc cgtcccgcgt acggggccgc ttcattgtcc tcttggaaag tttctggggc
     1921 ctcggctgga ttgcagcagc ctgcatcgcc tatttcttca ttccccttta tggctggcgc
     1981 atggccttcc tcatcggcgc cctgccggca ctctatgtct gcctgatccg cctgcatatg
     2041 cccgaatcgg tccgctacct gctggctcat ggccgcgtcg gcgaagcccg gcagatcgtc
     2101 gtctccctgg agcggcagct gcacgtaccc gtagcgccct tcgtcagcga aaaggaaacc
     2161 gtgccggtcg tcgccaaggc ttcgttccgg gaattgtgga aaaagccctt tgcgtcgcgg
     2221 acgatcatgc tctggctggt ctggttcggc atcaacttca gctactacgg catcttcatg
     2281 tggctgcctt cgctggtctt ccagcagggc tttaccgtcg tcaagacctt tgaatacgtc
     2341 ctcatcatga ccctggccca gctgccgggc tattactgcg cggcctggct cgtcgataag
     2401 atcggccgca agtataccct gtcgacgttc ctcttgttca gcggcgtcgc cagctacttc
     2461 ttcggccatg cgtcgacggc agcgaccctc atgatgtggg gctccgtcat gtctttcttc
     2521 aacctcggtg cctggggcgt cctctatacg tatacgccgg aacagtatcc gacggctatc
     2581 cgagccttgg gaagcggctg ggctgccggc ttcggccgct ttggcggcat ggctgcgccc
     2641 atgatggtcg gagccctatt ggcccggaac ttcggtttcg gcagtgtctt ctacatgttc
     2701 gccttggtct tcgtagccgt tgccgtcatc gtcttgagcc tcggcgtcga aagcaagcag
     2761 aaggacttgg aaagcatcag cgaaggactg gtcaaagcca agtaaggtaa tctcgaaaaa
     2821 aatgtaaaaa gctgtcaaaa tttgcttgac agcttttttt ttcaatgata aaatactttc
     2881 ccaagtttga cacttaaaaa agagtaaaag gctgaaaacc cagtaattac aag
//
LOCUS       DAWCYS010000210         2931 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1731_length_2931_cov_5.196453, whole genome shotgun sequence.
ACCESSION   DAWCYS010000210 DAWCYS010000000
VERSION     DAWCYS010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1731_length_2931_cov_5.196453"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          536 a          839 c          886 g          670 t
ORIGIN      
        1 gtcagggtcc gggccaggat atctttcttt tctttatacg agcggaagcg gttgaggacg
       61 tcgacgtgga cgccgaaggg accgaagcgt tccataaagg tctggaaatg ctgctgcgac
      121 aagaccgtcg tcggcaccag gacggcgacc tgcttgccgg ctgtgacggc tttaaagatg
      181 gcccgcatgg ccacttcagt cttgccgaaa ccgacgtcgc cgcagaccag gcagtccatg
      241 ggcgtcggct tttccatgct ggccttgata tcgcggacgg ctttcaactg atcttccgtt
      301 tcttcataag gaaaggcttc ttcaaattcc ttctggaacg gcttatccgg cggaaaggca
      361 tagccctgaa cgacttcccg cttggcatag agggcgacga gtttttctgc caggtcggta
      421 atggacttct gggccttggc ccgcgtcttc tgccagtcgg aaccgcccat tttctgtaat
      481 ttcggcgtat ccccttcgtt accgatgtag cgctgcaaca gggacaactg gtccgtcggc
      541 acgtagagga tatccttgcc ggcatagtgg atttccagat aatccttgtg gacaccatcc
      601 atttcaatgg tcttgatacc gatgtagcgg ccgatgccgt ggacttcgtg gacgacgtaa
      661 tcgccgggct tgaggtcggt aaagacgttg atttcctggc ctttggcggc atgatggcgc
      721 aggcgccgct tctgggtgcc gaagacatcc cgttcggcga cgacgacgat tttggcataa
      781 ggcagctcga agccgtcgtg gatctcccct tcgacaacgt agacgccgcc tggcgtactc
      841 tggtcatcat cgtagtcccg gaacggcagc tcatgctgct tcatccaggc ctggagcgaa
      901 gcccgtcggt cgcggctgtt gatgacgaag aggattgtat tccccaggtc ccgccaatgg
      961 tccacttctt cttccaggag gttgaactgc ttctggaaac tggccatcat cttggcggca
     1021 aagctggccg acgtgtctat catcatatcc ggcacggact gggccatgag cgacaagatg
     1081 agctgcggcc ccgggcgctg ggctgtgacc agatttttcc aggacgccag gcggccctta
     1141 tagtcgtcgg actccttgag gaccttcttc aggccttcgc ggacgcggtt cggttcatcc
     1201 caaatgatga cgccctgtcc ggcatagtcc aggagcgtat atttctcgtc atcctggaca
     1261 gtcagggaaa tgggcaggat gcgggccttt tcccgctggt cctgggattt ctggctgtct
     1321 gtatcgaacg tgcgcaggct gtccagttcg tcgccgaaga actccaggcg caggggctgc
     1381 ggttcgttga cggcatagat atcgacgatg tcgccgcgga cggaaaaatg accgcgccgt
     1441 tcgaccatgt cgacccgttc atagccggcg tcgacgaggt gctgcaacaa ctcgtcgcgg
     1501 tcgtagtcct gatggatggc cacgtcgata gcggcggcat cgatgcgctg cggcgccatg
     1561 acgtactggg ccgcttcttc cggtgccgcc aggatcagga gccggtcacc ctggcgcagg
     1621 cggcccaagg cttccatctg ccgggccgtc cggtccaggc tcttggccgt cgtcgtaaag
     1681 acggccttgt cgacgacagg gaaatccagg actggcaggt ccggtaccaa aaaggccagg
     1741 tccgccatcc aggccgcagc ctgttcccgc gacgggacga cgaggacggc ctgccccggc
     1801 ttttccgtca gggccttggc gacgagggcg ctcttgacgg aaccgctgag gccgtagata
     1861 ctatgacagc cgggctggga aaacgcagcc acggcggagc ggatattttc atcttgattc
     1921 atccattgaa agaattgctt catatcgttt ttacctcatg acaaattggg gctgtcgcat
     1981 gaagccctgg aaaacaaggc ggtccatgcg cagcccttat cgtgattcaa ctgcaactat
     2041 ttaattatat catagttcac tggcccctgc cagtagcgat tagaagaccg acgcgccctg
     2101 catgatgatg gcttcccgtt ctttccggct catcttgagg aaattcgtct tcttcagctt
     2161 atcttcataa gacgaatcca taccgttcat cttctgggcg acatccttgc ggatctgcgg
     2221 tagcaggctg tagaggttgc ggcccgggca gtccgtactg ttgacgtcgc gatggcctac
     2281 gatggtagac ggacccggca cgatgctgta gatcttgcag atccaggcga ccagtccttc
     2341 cagggagtag atctgggccg gcgtggggat ttccttttca aaattgcccg tcacattgat
     2401 gccgacggta tggtagttct gcccttcggc atgagcaccg accgtagcca gcgggcggcc
     2461 ccgttcaatg gtcccgtctt tgcggatgac gtaatggtaa ccgataccgg cccagccgtt
     2521 ggccagatgg gcgcgatgga tagccgctgc cgacgtatag aaatctggga ttcacacgtg
     2581 atggatgacg accatctcag tattcgcacg caggagcagc gtttcgcgga actggaaatg
     2641 cgtttccttg atggccatcg gttcggccgg cgccgcatcg gtcacgggat tatagttgcg
     2701 catgacgccg cgggctccgg cgggtttcgc cgaccgcggg acattgccga tgactgcggg
     2761 cttggcggct gtgctggctg cagcggctgg ctgggacgat acaggcgccg tcttgccggc
     2821 ctggtctttt ttcgtcttcc ctgccttctt ggcagcctta gcggctttgg ccttggccct
     2881 ggctacggca tccatgtgct ggtcgacact ggcgacgccc gtcgtatagg c
//
LOCUS       DAWCYS010000211         2915 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1741_length_2915_cov_4.091608, whole genome shotgun sequence.
ACCESSION   DAWCYS010000211 DAWCYS010000000
VERSION     DAWCYS010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_2915_cov_4.091608"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          596 a          759 c          842 g          718 t
ORIGIN      
        1 cgtatatatt gacaatattg gggtgtgaca atttggcagc cgcctgggct tcctggcgga
       61 agcggacgac aaaatcgtgg tcgttgccgt attttgaatg aagtattttt acggcgacga
      121 tccggtccag gaggatatcc tgtgctttgt acacgctggc catgccgcct gtgcctactt
      181 tttccaatat cttgtatctg ttatctaaga tatgaccaat catttttagc tattccacca
      241 ctttcatagt cgttcccgat tatatggttg ttaaaattgc cgtgatatta tctttcgccc
      301 cgttttcata gacgcgggca aagagatcat tgatgatttc ccgttctgtc cggcggtaat
      361 cgccgatagc atcgtagagt tcctgtttct ggatgaatga tgtcaagccg tcggaacaaa
      421 tcaggatgcg atctttcggc tgtacttcaa aggctccgct gtcgacgacc aagttctcat
      481 cgacgccgac ggctctggtc agcacatggc gctggggatg atccatcgct tcttcttcag
      541 ttatcttgcc cttttcgagc aggacctgga cgatcgtatg gtcgacggaa atctgtttga
      601 attccccgtc gcggtataag tagatgcggc tgtcaccgac gtggccccag aaggccatat
      661 gacgagccag ggtcaccatg gacacggtcg tccccatgcc cttgagcgaa gggtcgatca
      721 aggtcttgct gaggatgctg gaattcgcat attgaatggc tttctccagc tgtaccggtg
      781 ctgcataact aaagttcttg aaataataag ctatcgcttc tacggccgtc ttgctggcga
      841 tttcaccgcc tacatagccg cccataccgt cggctacggc cagtacgttt ttctcgtggc
      901 tgatataaaa ggaatcttca ttcactttcc gaaccaggcc gattttggat tcaccgtatg
      961 tgcccaaatt ctcacgtcct ctcacggtgt ttcgttatag gtacacatta attcatatca
     1021 tattcgccgt cacctgtcaa attttttcgc gttttatgcg cagctggccg caggcggcct
     1081 gtatctggct gcccatttcc cgccgcagag tgacggaaac gccgtggcgc tgcaaatacg
     1141 tgacgaattc attcatcgtc ttttcatcgg gccggtagag gccgacgtcg tagttgtcgt
     1201 tgatgggaat ggcattgacc aggatgttct gaccacccag gagacgggcc agttccttag
     1261 cgcaggcgtt ggtacaagtc aggcccttga tgaggatgta ttcaaaggtg accttccggc
     1321 cagtgacatc gaaatagtgg cgggccgcct tcaggacttc gtcgagggga tatttctcgg
     1381 caatcggcat gatgcgacgc cgcaaggcat cgttcggcgc gtggagggat acggccaggg
     1441 tgatgggcat gccctctttg gccaggtcgt acatcttggg gacgatgccc gacgtcgaca
     1501 aggccatgtt gcgatagccc agatagagcg tatccttttc gtggatgagc ttcatgaagc
     1561 ggatgacgtt atcgtagttg aggagcggtt cccctgtccc catgatgacg atagaatgga
     1621 ggtccggcgt gacgtatttg cggaaagcca ggagctgggc gaccatttcg ccagccgaca
     1681 ggttgcggac gaagccattc ttggcggacg cacagaaggc gcagttgacg gcacagccga
     1741 cctgggacga tacacagatg gaattgccat agtcgtgctt catgaggacc ggttctacgg
     1801 tcgcaccgtc agccagctgc aatcagagct tcactgtctg tccgtccggg gcgtccaggc
     1861 gggacacgac ttccgggaaa tagattggca gcacttcctt cagctgttcc cggtctttct
     1921 tgggcagtag gaccatatca ttccaggaaa agatattttg cttataaata taatgaaaaa
     1981 tctggtcggc ccggaacttt ttcatcccct gggcgacgag gaacgcctgg agttcctttt
     2041 tggtcatatc aaatacatta ctcatgtcag ccctcctttt tcatgcgggc aatgaagaaa
     2101 ccatccaggc cgtcgcgctg gggcaggagc tggatgaaag gcccttcatg gccgggaacg
     2161 ggaagccact ccgtcccgtt ttctacgtgg aattccggat gattcttcaa aaaggcagcc
     2221 acgatgcggc tgttttcatc atcattcatg gtgcaggtac tgtagaccag gatgccgcca
     2281 ggacggacgc attgggatgc actgtccagg atgcgccgct gcagggccgg cagggtgcgc
     2341 aggtcactgg gattcttgcg ccagcgcagg tcgatcttat ggcgcaggac gccgaggccc
     2401 gaacaagggg catcgaccag gacccggtcg gctttgccgg cgtacttctg gccgatggtg
     2461 cagccgttct ggagcagggt ccgcacattc ttgaggccca gtttctgggc attatattcg
     2521 atgagccgca gtttatgctc atggatatcg ccgccgtaga cgacacagga cgggccgccc
     2581 agtgtggcca ggtgcgtcgt cttgccgccg ggagcggcgc agacgtcgaa gatgacttcg
     2641 tgcggctgag gatcgacgat atgggctacc agctgggacg gttcgtcctg gatgatggcc
     2701 cggccatcgc gcaggcatgt cagttcatgg atgccgggat tgccgtcgag gtagatgccg
     2761 tctggtgaaa tggcagccgg gcgggccggc aggccttgtt tttccaaatc ggccagcagc
     2821 tcttcgcggc tggtctgggc cgtattgacg cgcaggcaca gcggcgggat cgtatcgaaa
     2881 gattggcaga gccgttccgt atcgtccggc ccata
//
LOCUS       DAWCYS010000212         2909 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1743_length_2909_cov_4.502102, whole genome shotgun sequence.
ACCESSION   DAWCYS010000212 DAWCYS010000000
VERSION     DAWCYS010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_2909_cov_4.502102"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          672 a          729 c          756 g          752 t
ORIGIN      
        1 ccttccttct ggtcggcggt ggagaagcag aggccgaaga cttcagcttc ataagcgata
       61 ccggttacga cgtcgcagtt gatgccgcgg ttgatggcag ctttggagag ctgtacagca
      121 atcggagcgt tcttagcgat cttcttagcc agtttaacag cagccggcat taattcttcc
      181 tgcgggaaga cttcgttgac caagccgata gcttttgctt tttcagcgtc gatcatgccg
      241 cccgtgtaga tgagttcttt agcgtggccg cggccgacga cacgggtcag gcgctgtgtg
      301 ccgccgaagc ccggagtgat gcccagaccg acttccggct ggccgaattt agcgttttca
      361 gctgcatagc ggatatcgca agcgcaggag agttcgcagc cgccgccgag agcgaagccg
      421 ttgatagctg cgatgacagg ctgcggcagg ttttcgattt ccgtgaagac gttctgaccg
      481 aactggcccc attcgcggcc ttcagcagcg ttcttggaag ccatttcttt gatgtcagcg
      541 ccagcgacga aggatttagc acctgcgccg gtgatgatga cgactttgat agctttatca
      601 actgcgactt tttcgacaac gtccttcaat tcgctgaccg tagcctggtt cagggcgttc
      661 agagctttcg gacgattgat ggtgatcgtt gcaataccgt cttcgacagc aaaaagaatg
      721 ttctggtaat ccatgagaaa aacctcctaa atacatataa tagtgttaat agtatagtat
      781 agaaatagta tgtacaacag gctgccctcc ccaaagggac tcctgccagt cagaccgccc
      841 ctgccgacag gataggcgca ctgtcaatac gcgcatgaga ttctaaaaat aatacggggt
      901 gtgaaagtca cgccccgtat tcgattagca gatcgctaac ttatttcgtg taatcgtaga
      961 agcctttgcc agtcttacgg ccgaggtaac ctgcctggac atatttcttg agcaatgcgc
     1021 aaggacgata tttcggatcg ccgaagcctt cgtagaggac ttccataacg tggagaacga
     1081 cgtcgttacc aataaggtcg cagagagcca gaggtcccat cggatggttg aagccgagtt
     1141 tgcagacgtt atcgatgtct tctttggaag caacgtcttc catcaaagcc tggatagctt
     1201 cgttcagcat cgggatgacg atacggttgc cagcgcagcc cgggaagtcg ttaactttga
     1261 ccggtgtttt gccgaggtct ttcgaaagtt tgtcgatctt ttcatacgtt tcttcagaag
     1321 tagcgatgcc gttgatgact tcgatcaatt tcatgaccgg tgccgggttg aagaagtgca
     1381 tgccgatgac tttgtccggg cgtttcgtag ctgcgccgat ctgggtgata gccagggacg
     1441 aagtgttgga tgcgaggatc gtgtgttccg ggcagagttc gtcgagttct ttgaagatag
     1501 ccgatttgat cttcaagtct tcgatagctg cttcaacgac gaggtcgatg tctttcatgt
     1561 tatcagccgt acgggttacg aagccagaaa cgcgagccat agttgcgtct ttgtcttcct
     1621 gggtcatttt gcctttggag acctgtttgg tcagctgttt ttcgatttta gctttgccgc
     1681 cagcgatgaa ttcgtcttta atatcgttca agacgacatc ccagccgtgc gttgcgaaaa
     1741 cctgtgcaat gccagaaccc attgtacctg caccaataac cattacttta ctcatgttta
     1801 actcctcctt cgtcgcgtga tttttttcac tagattcttc taaaaaaatc acgcacgttc
     1861 taaataagat tcactgctta cactgatagt ataccacact tcccaaaaaa atccactaaa
     1921 aaacggcact tttttcaatt tttcacattt atgacatgcg ttttatgcaa taatcaagaa
     1981 gtcaaattac ttctttgcat cattcgatag aattatctca tagatctatg tgacgttatg
     2041 aacttacgtc atgggagata cttctatttt tcacaaagtg catgggtagg gattcccttt
     2101 gggaatcccc acccgcaggt cgccgcgaca tcaaagatgt cgcatgtagg ggccgcccca
     2161 aagcctttcc agcccgtcag gtgctgacca acgggaggca caggacagct gctggaacgg
     2221 acagccgtgg cgggcggccc gcttgtggga attccgttta cggatggccc gcttgtggga
     2281 ataccgcaaa aaagaggctg tcgcattaag acagcctctt tttgagtttt aagtttgtag
     2341 tttttagttt gcagcaaatg gtaaaaaatt taaaaccaac ttctacaaac tataaactac
     2401 aaactccgta cgataaacta tcacgaaagg acaacctctc agtcagcttc gctgacagct
     2461 ctcctatgag gagagccgaa cggaccgccc ttcggatagc gttgttcacg ggattcaggc
     2521 gggccgcctt atcaggacgg cccctacgaa aagccccaat cggggccatt tttacgagtc
     2581 gaaaatcacc caaggcgatg ctttcccttt gaggcgctgc cgtgcaacgg catccgtagg
     2641 ggccgtccca gagggcggcc cgctacgcgc tgagggtatc ccgaagcgtc gttctcattt
     2701 tgagtctgtc gcggcgggcc gccgcatcgg aatccgaagc ggccggcgaa ttacggatag
     2761 cgttgctcat gggattcagg cgggccgccc tttgggcggc ccctacgagc cacgagccac
     2821 gactcacaaa gtgccgttca gggctttgag caggttttcg cgtttgtcat aggccctgcg
     2881 gtaggtagcc gtatggtttt cgtcgggca
//
LOCUS       DAWCYS010000213         2903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1747_length_2903_cov_4.916433, whole genome shotgun sequence.
ACCESSION   DAWCYS010000213 DAWCYS010000000
VERSION     DAWCYS010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1747_length_2903_cov_4.916433"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          663 a          801 c          885 g          554 t
ORIGIN      
        1 gaaaagacat agggtggatt tcgcaggcat cgaacctgcg aaatccatcg ccattcatcc
       61 cctacctaag aggaaggagt cttctggcga aactgtgata aagtttcccc acaattattt
      121 tacaccatct atcttgtgca ggaaaatcca gctccccagg ctgaggaaaa tgacacccat
      181 gaccgtcagg gccgtcaggt tatggctcag cgtggccaga tggccggaca ggaagagttc
      241 gcggacggcg ttggacatcc aggccatggg aaaggcctgg gcgacgacct gggtcactgg
      301 gtccatggct tccaggggcc acgtatagcc gccgaagatg aaggccggca cggtgtacat
      361 gatggacgcc cggacgaact gcaattcgtt gtggaaaagg gccgacgcga agaggccaag
      421 ggacacggcg gcaaaagaaa agtccgacgc aagggtcaag agggccgtga ccgatgcccg
      481 gtcgggaatc cccaagccgt aagaaatgac gcagaccagg gcaaaggaca agacggacag
      541 cagccagtac acggcgacct tgacaaccag gacggccgcc ttcggtgcgc ctttcaggcc
      601 ttctgtatcc ttgaaatcgc cttgcacggc ggcgccgaca gccaggaaga tgccctgctg
      661 catggccgcc atggccaggc cgaccatgaa gaacatcatg taactctggg tcgggttatt
      721 gaggacgcgc agtgtcgtct ctaccggtga gatgcgcttc ttcagcatct gttcatcgcc
      781 gctggtccgc aatgccgcct gccgggcggc aaagcgcgta ttgaaggtat tgacgatgtc
      841 gttgccagcc gtcgtggcga tattggtcgt aatgatattg gccccgttga gcatgaacag
      901 gactttagcg ctgcggccgt tgaggatatc gtgagaaaaa tcggccggaa tgatgacggc
      961 ggcgtagacc tctttccggc gcagcatgtc ccgtgcctcc ccttcatcgg cgacgacgcg
     1021 gaccagcccc agccgttcgt cctcatcgac ctggcgggtc aaaaggcggc tgtaggccgt
     1081 atgttcctca tcgcagacga ccgtcggtat ctccttgacg acgccgggat aatagaggat
     1141 gccgaagaga atcaggtagg ccgtagcggc gccaaagaga aagatagccc gccgggcatc
     1201 gtggagaaag gcattggcca gttcccgtcg tatcaatgca ataaatccca ttatgaccgc
     1261 gctcctttcc gcttacgcgc aatagcccct gccgctattt ccaggaacgt cttcatgccg
     1321 tagcggcggc gcagggcaaa gatagccgtt cccaggacaa aggccccggc ccccatgagc
     1381 agcagctgca gggactggga aaccagcgcc ggcgcataac cggccaataa gatatcgcgg
     1441 acgctgtcgg cagcatggcg catgggaaaa atctgggcaa tggcggcagc gacgtcgctc
     1501 atgtagaaat caggccagct caggccgctg tacaagaggc ccggcatgat atagagcaag
     1561 ggcatttcga tggacatgac tgcatccgga ctgagcacgg caaagaggac cataatgctg
     1621 gcgacggcca ggcagaaagc cgttatcatg acggccaggg cggccaggga accctgtacg
     1681 gggacggcga agctgtagta taagaatccc agggagagga aaaaagaagg aaccgccatg
     1741 accagtacac agagcacctt ggcgcccagg atcagccacg acgggatgga cttctcgtaa
     1801 ccatgatacc ggaactccct ggcgatgagg gggcacaaga cgagcataat ggcaatctgc
     1861 aggccattca tggtcagacc ggccagcatg aacggcgtat agccggccgt cggattgttg
     1921 acgatgcgga tgcccaggcg gacggggtaa gccatattca tggccgctgc cggcagctgg
     1981 ttgcccgctt ccaggagttt ggccgcaatg ccgacgaaca aggtcttgtt gatttcactg
     2041 gctgccgtca gggcggcgtt gccgaacatg ttattggccg agttgaccgt cagcatgagc
     2101 ggcacgggat tgcccagctt gacctgtttg gacaaatcct ccgggatgta caggccgaca
     2161 agggcctagc agctgttcaa atcatcgacc ataacttccg accgcgtcac ctggtcgacg
     2221 agctggaact tttcagcatc gctgtaagcc tggacgatgg tccggctgac gacggactgc
     2281 tcttcgtcgc agatgaccag ggggatccgt tcgaccgagt tctgatagta aatgatacca
     2341 aaggccaggg taaaggccag gtgcatcagg aagacgatct cagcgacggg aaagctcccc
     2401 tcacagagcc agtgcgattc ctgcttaaag acgtgacata ctttcatgtc agtcacctgc
     2461 catatactgg aaacgcatgc ccggccgcag ggcatcgctg ttgacctgga ccttgacgtt
     2521 gaaactgatg atatccgtat cgttgccgcg ttcgctcgtc gcccgctggg tggcgaattc
     2581 cgatttctgg ctgatatcgg tgatggtccc ctggacgcgg gtgacgccgt cacgggcctg
     2641 catggtcagg acctggccga gatggaagtc tttgagcttc gtttccggaa tcttgaagtc
     2701 gacccagttg tcgttcgggt cctgcagggc gaccagcggc gtccccaggg aaatcatgga
     2761 accttcttcg atgtatttag ccgtaatgat gccgtcgaaa ggggccttga tttctgtttc
     2821 atgcagggat acttcgatct gctgcagcag ggcctgagcc gcttccagtt tcttcttggc
     2881 cgcatcggaa gccgctaagt tgg
//
LOCUS       DAWCYS010000214         2901 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1748_length_2901_cov_3.756852, whole genome shotgun sequence.
ACCESSION   DAWCYS010000214 DAWCYS010000000
VERSION     DAWCYS010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1748_length_2901_cov_3.756852"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          784 c          886 g          684 t
ORIGIN      
        1 tactacaaac tctaaacttt atttcaagtg tttcaatcct ttggcgccga tgtcgctgcg
       61 gacttgctgg ccgtcgaagt ggatgcgtat gacgttggca taggcctttt cgatggcgct
      121 cttgaggtcc cctgctacgg ccgtaacgcc gaggacgcgg ccgccgttgg tgacgtactg
      181 gccgtctttc ttggccgtgc cggaatggaa gaccatgaca tcttctttat cgacagcatc
      241 gaggccgctg atgacatcgc ctttatggga cgaagcggga tagccggcag aagccatgat
      301 gacgcagcag gatgcagcgt ctttccagtg gaccatatcg gccgtcagcg tgcccttggc
      361 gcaggcatac atgatctggg ccaggtcgcc gtcgaggagc ggcaagacag cctgcgtttc
      421 gggatcgccg aagcggcagt tgaattcgac gactttcggg ccatcggccg tgatcatcag
      481 gccggcatag aggcagccct ggtaggtgat gccttcctgt ttgaggccat cgacgacggg
      541 tttcagaatc ttttcttcga cttccttgcg gatttccggc gtaacgaccg gtgccggcgc
      601 ataagccccc atgccgcccg tgttggggcc ttcatcgtta tcgaagatac gcttgtggtc
      661 ctgggcggaa atcatgggca cgatggtctt gccatcgacg aaagccagga ggcttgcttc
      721 ttcgccgacc atacattctt caatgacgat gcggccgcca gaagcaccga agatgtggtc
      781 ttccatcatg gcgttgacgg cttcgatggc ttctgcttcc gtctgggcga cgacgacgcc
      841 cttgccggca gccaggccat cggccttgac gacgatcggt gcgccctgtt ccttaatata
      901 ggccttggcg gcttcgccat cggtaaagga ggcgtaagct gccgtcggga cgtggtattt
      961 cttcatgagg ttcttggcaa agactttgga cccttccaac tgggctgccg ccttggtcgg
     1021 gccaaagaca gccaggcctt tggactgggc caggtcggca atgccttcgg tcaaggggac
     1081 ttccgggccg acgacgagca tgtcgatgcc ttcgtttttg gcatagtccg tgatggcgtc
     1141 caaatcggac agggcgatgt cgacgcactg ggcgacttca ctcatagcgg cgctgccggg
     1201 aatggcatag agcttatcga cgctcgggct ctgagccaat ttccaggcca aagtatgttc
     1261 gcggccgccg ccgccgatga ctgctacttt cattattttg cggccccctt ctgaacctgt
     1321 tctgccaggt atgcggcaat ggccgtatca tagacagccg tgtgatggaa ggcttcctga
     1381 gccagttcca ggcggtactg gtcggagaat tcgccgtcct tctggaggat ggaaatgatt
     1441 tcgccgtatt tggacgggtt ggtgacgatg gcgacgtatt tgttattctt tgccgaagcg
     1501 cggaccatgc agggaccgcc gatgtcgatg ttttcaatgg cttcagccaa ggtgacatcc
     1561 ggtttggcaa tggtctgctg gaacggatag aggttgacga cgaccaggtc gatcggtttg
     1621 atgccgtgtt ctttcatggc tttgacgtgt tccggattgt cgcggacagc caggatagcc
     1681 ccgtggattt tcggattcag cgtcttgacg cggccgtcca tcatttccgg gaagcccgtg
     1741 acgtcctgaa cggcgatgac cggtacgccc gcttctttca gggtccgcat ggtgccgccc
     1801 gtggaaatga tttcaatgcc gaggtcagcc agggctttgc ccagttcgac gacgcccgtt
     1861 ttatcagata cactaatgag tgcgcgtttt actttcatga aaaattcctc ctattcctga
     1921 gtccctttga cgacattgcc gtcgatagtc aatttatcat cgcaaaagag cgctgccgct
     1981 ctgacataag tcggatgttc cttggtgagg atccgggctg ccagcgtgtc ttccgtatcg
     2041 tcttcgtaga ccggtacggc tgactgcaag atgatcgggc cggcatccat gtcgggaacg
     2101 acgaaatgga tcgtgcagcc agcaatcttg acaccggctt cgacggcctg gcgctgggcg
     2161 tggaggccgc ggaacgacgg cagcagggcc ggatggatgt tgagcatctt gtgttcataa
     2221 tgggaaatga agtcaccgct caagagacgc atgaagccgg ccaggcagat gatgtcggcc
     2281 tgatacggtt caatggcgtc gaggatgtat ttttcaaagg ccgctttcga gtcgaaggat
     2341 ttgcggtcgg ccaaaatgac cggtacgccc cattccttag cgcggtccat gaccggtgcg
     2401 cccgggatat cgcagaccag ggccttgatt tccgcattga tcgtgccggc ttcgacggca
     2461 tcgtggaggg cgatggcgtt ggagccacgg cccgatgcga aaatgaccat cttcttttta
     2521 gtcatggaat acgcctcccc gcaaggttac gggtacgtcg gaatcgacga ctttgccgat
     2581 gacataggcc ttttcatcgc ggtcggccag gttcttgagg acggcatcag cgtctttttc
     2641 atcgacaatc atgatcatgc cgatgcccat gttgaaggta cggtacattt ctttggcatc
     2701 gacgttgccc catttctgga ggagtccgaa aatcggcagc tgcggccacg ccgtgacgtc
     2761 gatatcgacg ccgacgcctt tcggcaggac gcgggggatg ttttcataga caccaccgcc
     2821 cgtgatgtgg cgcatgcccg ggacgggaaa gttcttgagg atcggcacgc gcggccgcgg
     2881 atagaggcgg gtcgccgtga g
//
LOCUS       DAWCYS010000215         2896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1752_length_2896_cov_3.872932, whole genome shotgun sequence.
ACCESSION   DAWCYS010000215 DAWCYS010000000
VERSION     DAWCYS010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_2896_cov_3.872932"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          719 a          727 c          684 g          766 t
ORIGIN      
        1 tatataacag taataaagtc ttcaaaaaaa cactccttcc ggcaagatgc tcattgcaag
       61 acagcctgca agaacatggc cgtactgaaa tcatcattga cccggcgaag ttcacgttca
      121 tacgtcggca gcatagcttt ataggaattc aggaactgct ccaactgctg atgcaggtgc
      181 tggagatccg tcaaatcgac ttcctgcccg atttggtatt cagccaagat ttccggattc
      241 agtacgggcg tcgccaatac tggcttatag agcgccagag cattaaagta gacggcgctc
      301 cagttatata ggtaccgcgc attgctatac ggcagaaaaa ggacatctac actttgcagc
      361 aagtcatacc aggcacggcc ctgaaccttg ccctgtatga aacggactgc gtcttcatgc
      421 tcatatttct tcatgatacg ttccatatcg gccgcatcat ccggtcccgt cggcgccgct
      481 tggacgacaa gttctgccct gcctgccaac tggcaactga caaaagcctg gatgatgccg
      541 tcgacatcct tttccccttt ccgataatgg ccgaaaaagc cgatgcgtac gggttcacga
      601 tatgtcaaat taggctgcac cataacgcct tgtgggatga ggactggtgg atgaattaat
      661 tcaatattgg atacgttgtc ttctttaaaa tcattccgca acgtcgtaat cttcagcttg
      721 atatttggat aagcctgaca ccgccgagcc tgcttgagga atttcggttt ttcttgcgga
      781 ttgacgccta aaaaaatgaa gatgactggt acagggctcc actttaattg gctcttatgc
      841 agagatctca ggtagcgata cgtcgctgtc gtcaagatga tagcatcgac ttcatgacgg
      901 caagcctttt cataagctga attgaaccaa cgaacacgac gtcgttcccg ttggatagac
      961 agccagattt tctttacctt ccccgcccct tcataatcga cgatttgccc gcctttcaaa
     1021 tattcgatgg tgataccgaa attaacagga aggctacagt gtaccggcaa atctaagacg
     1081 ggatgatgcc cctgcttctt caattcatca ataatcaggc gatcgaattc ctgttcaaat
     1141 ccggcctgtg tttttactgt ctctaaaatg gctacgcgca ttattatttc tccgctctat
     1201 gcaactcgta tagtttcaaa tacttcaaca aagtataaaa aaagtggttg aagtacatat
     1261 atgtccccat ccacccgtct aaaaaaccga cccgtgatac gaacattttg aagaacccgc
     1321 ctaaactgtg caggaagata ctactcaggg aaatccgttt gccccgctta taggcttctt
     1381 cagcccagat ggttgtatac tggcagagtt ttccttccca atggtgccag ttcttatagg
     1441 tataatgttc aatatggcct ggtaaaaagc ggagcgacaa atcacattcc ggatgctcat
     1501 gaactttatg gacccacgta accgccgttc tgggaaacat acgggctaca tggtccggtt
     1561 tcaagacgcc atgattgaat tttgtcccaa aagcgaccga cttccgttcc atggaaaact
     1621 ggcagtccag ctttccttca tctacaacag ccttaacaga ttgaataagc tcatcattca
     1681 accgttcatc ggcatcgaga tacagcaccc agtctgctgt ggtctgttcc aaggcgaaat
     1741 tgcgctgggc tgcgaaatcg tcggtccagg cacggaagca aacttttgcc ccatgggctt
     1801 gggccagttc tacggtctta tcggtactcc cagagtcgat gaccaggact tcgtgggtgc
     1861 attttttggc attgtccacg acgtcgacga tattgtcttc ttcatttttt gtcaaaatca
     1921 atatggctaa agagctcatg ggcagcgctc cttctctctc aatatttatg tctgatacgc
     1981 attcataact atgtaacatt gtaccatatc gcccggtcaa agtacagcca gccgcagttc
     2041 aataaagcaa gggctgcctt tcccagcatc cgcccggaga gacagccctt tttataactt
     2101 cgcttgtgtt tataattctt tgatgaggtt gaccatttcg atggcgctgg tagcgacgtc
     2161 gaagcctttg ttgccggctt ttgtgccagc ccgttcgatg gcctgttcga tattttctgt
     2221 cgtgatgacg ccgaacatga tggggatgcc cgtcttgagg gatgcctggg cgatgccttt
     2281 ggccgattca ccgcagacga ggtcatagtg gctggtagcg ccgcggatga cggcaccgag
     2341 gcagatgacg gcgtcgtatt tgcccgattc tgccattttc tgggcgatca gggggatttc
     2401 aaaggcgccc gggacccagg ccgtcgtaat gtctgcgtca tcgacgccat ggcgatggag
     2461 agcgtcgtaa gcgccgccga ggagcttgct gcagatgaat tcgttgaaac gtgcgacgac
     2521 gatgccgaac ttcgcaccag aaccgattaa tttaccttct ttgacttgta ccattgtaaa
     2581 acactcctat tctgaaatat tatgttaaaa tccattttcc agcagttttt ccatcgtcag
     2641 gcccgaagac gccgacgacg gatgaccgac ggtaaagcta tggacatatt tcccgatgac
     2701 atccgtttcc aagttgacga tagcgcccgg atgcttatcc atgagcgtcg tattggccat
     2761 ggtatgggga atgatggaaa cggaaaattg gctgctgccg acggatacga cagtcaggct
     2821 gatgccgtcg atggtgatag agcctttttc gacgatgaac ggctccatgt ccttggggat
     2881 ggaaatggtg atgacc
//
LOCUS       DAWCYS010000216         2894 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1754_length_2894_cov_5.086298, whole genome shotgun sequence.
ACCESSION   DAWCYS010000216 DAWCYS010000000
VERSION     DAWCYS010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_2894_cov_5.086298"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          591 a          791 c          913 g          599 t
ORIGIN      
        1 ccgtttcgat tgaccacaga catccggcgc acgataccgc ctgcaagagg aatgacgacg
       61 aacaggcaga tcctgacaaa gaggacacta taaggaaccg caacttgtga aactcctaaa
      121 aggaacttga cgataggtac gaaagcgatg agaatgatta aatcattcgt cgcgacttgg
      181 acgacggtat aggccggatt ccctttgacc agtgtactcc agacgaaaac catggccgta
      241 cagggagccg caccgagcaa tacggcgcca gccaagtatt gccggcccag gtccgcaggg
      301 atccacgaac caaagaggac gaaaaggaag aaccaggcaa tagcatacat ggtaaaaggc
      361 ttgatgagcc agttggcaat ccaagtgacg aacaagcctt taggctggcg tcctacggct
      421 ttgatgctct gaaagtcgac tttcatcatc atcggataaa tcatgagcca aatcaaaatg
      481 gcaatgggaa tagaaatccc catgaccggt aaggtatcta aaaaggctgg cacagccgga
      541 agggcataac cgatggctac gccgacgacc atgcataaca agacccaaag ggtcagatat
      601 cgctgaaaaa aactgatgcc tgcgtgtgat tcatgttcca tgataacgcg cctcctataa
      661 atagataaat tgaaatatat gaatgtttaa tatctgaaaa agggcttgct acacgatgac
      721 aagccctttt actacaaact acaaacgctc aactacagac tataattttc ccaggacttc
      781 cccgcggtct tcgtcggggc gccaggcgat gagggccgtg tggccctggg ggtggtcgtc
      841 gactttgttg atccaggcga tttcgtggga cgctttggct tttagcaggg ggctcgtcgt
      901 ccgggcctga tagaaggatt tgttgatgac ccaccatttc gagcgcagtt tcgtccgctt
      961 caggtccgct tccaggcgca gggcttcata gaccggcgtc gcttcgggca gggtgacgat
     1021 gacgacttct gtttcgtccc ctttcaggcg cggcagcagg tgctgtactg acggcggcgt
     1081 cgaaccgtgg gtccggcgga tttcccggtc gtagctttcc gtcgattcca ggagcagcag
     1141 cgtgtggcct gtcggcgccg tgtcgatgac gacgatctgg tcgtcggccg cttcgacgat
     1201 gtcggcaaag gcatggaaga cggcaatttc ctgggtgcac ggcgagcgca ggtcttcttc
     1261 gatgtaggcc aggtccgacg ggcccaggcc gttggctttg gcctggctca ggacttcatc
     1321 ctggtatttc ttcagctctt ccccttggtc gatatggctc agggtcaggt tgtccgtctg
     1381 gctgacgacg tactgcaagt gagcggccgg gtcggtcgtc gtcaagtgga cgcgatggcc
     1441 gcggcgggcc aggcccaggg cgatggcggc agcgacagtc gtcttgccga cgccgccctt
     1501 gcccatggtg aagatgacct tgcgctgatg ggcttccagg tcgtcgatga cggcctgcag
     1561 gggctggacc cgttccagcg tcagttccgt cgccgcttcc ttcacttccc ctttgagcag
     1621 ggcccggacg tggtccaggc cggtgatgtt gtacgtccgc aagggaatct ggaagcgcgg
     1681 caaatcggcg atggcttcgg gtatggcctt cagggcggcc tgctgttttt cgtataacgt
     1741 cttggtcgca tcgtcatccc acgaggccaa caggccgttg acgatgagct gctggttggc
     1801 aatgcccagg gcggccagtt ccttcgacgc ccgggccgct tctttcagcg gcagggcatc
     1861 gggacgggcg acgaggagca tcgtcgtcct gtccccgtcg gccagggtct ggacggcctg
     1921 tttatacagt tctttccgcg attccaggcc cgacagctgg cccaggcagg acgcgccgtg
     1981 tttgctcttc tggatgaact ggctccacgc cgacggcagc tgcagcatgc gcagggtgtg
     2041 gcctgtcggc ggcgtatcga agatgatgtg gtcgtagcca caggcaacgg cttcatcggt
     2101 gaggaaattg gtgaactcgt tgaaggcggc gatttctacg gtacacgagc cggacagctg
     2161 ttcttccatg ttgcgcaggg ccgcttccgg caggaggccg cgatagggcc cgatgacgct
     2221 ttcccggtat tcggccgcgg cctggaccgg gtcgaggttg gctacggcca agttgggcac
     2281 ggcggcgatg ggcgtggcct tattggtcag ttccatggaa aagacgtctt gcagattcga
     2341 cgccgggtcg gtactgatga gcaggacctt cttaccggca tcggccaggg cgacggccgt
     2401 ggcacaggcg acggacgtct tgccgacgcc gcccttgccg gtcaggaaga ggtatttcgt
     2461 ctggacgacg tgaaagggat cgtagaggat catttacagt cacctccgca acagcacgac
     2521 gggctttccg gttccgccgg cgtgtcaccg cagcagcagg aagctgtttc ttcggcgctg
     2581 tcgccgcagc agcagctgtc cccagcggcc tgagcggctt cgaggatggc cgggtcgaag
     2641 ccgatgaggc tggcaaattc atcattcgtc ggatagcggc cggcaaactg gacggcgccg
     2701 tcgacgagga tgaggggcag gtcttcagga ccgtggtact ggagaaagtc cgtcacggcc
     2761 ttggtctgga cgaatttcaa gggcgcacta gtcaggttat agcggccgac gtcgatgccg
     2821 tgctgtttca gcctgtccac gaccgtcgat acgcgcaaga gttccggatc gatagacgga
     2881 ccgcagagac cggt
//
LOCUS       DAWCYS010000217         2884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1769_length_2884_cov_4.881584, whole genome shotgun sequence.
ACCESSION   DAWCYS010000217 DAWCYS010000000
VERSION     DAWCYS010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_2884_cov_4.881584"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          566 a          840 c          939 g          539 t
ORIGIN      
        1 gtgatatccg ggtcgtcgcc actgtccttg cggatggcgc acgtcgccga ctggggaccg
       61 aaagagacgt cttccgggat gagctggagg accgtcccct gcggcgtctg caaggatatg
      121 gcttcgacgc ggcgcccggt caggaccatg atggccgccg ccttggccgc cgcggccgca
      181 caggacccgg tcgtatagcc gcagcgcatt ttcttccctt ctttgaccgt gaatagtgac
      241 atggtatatt tctcctttta tcgtttttcg ttcatcgttc gtcgtgatgg atggttattt
      301 ttttaaacct tacgtcaaat gatgaacgta tttaacaaaa cttcactgat aatctgacgt
      361 gtcattgcaa cacaacgatg gccgaacggc cgtcactggt ggtgacgatg tcggtccgga
      421 cggaaaagac gtcccaaagc cgttccggtg tgaggacggc ctggggtggt cccgtacaga
      481 ccagggctcc gtctttcatg atgacgactt cgtcggcata ttgccaggcc tgattcatgt
      541 cgtgcaggac gacgacgatg gtcttctgcc agccctcatt gatgcggcgc aggaggtcga
      601 ggatgcgcag ctggtggtgc acatcgagat acgtcgtcgg ttcgtccagc aagagtatgg
      661 gcgcatcctg ggccagcaag acggccagcc aggcccgctg ctgttcgccg ccagagaggt
      721 gctgcatgac ctcgccagca taggcttcga ggccgacggc ctggatggcc cggtcgacgg
      781 cgtcccggtc ttcccggctc cggccgctcc agagacggcg gtaaggaaag cgccccatgc
      841 cgactaagtc gcggacggtc acatcaggcg gcacgtcatg ggtctgggtc aggaaggcca
      901 tttcccgcga caaggcctta cgggaaaaat catggacctc gcgctgaccc aggcggatag
      961 agccgctggc tgcgggaatc atgcagttca cggccttgag gaaggtgctc ttgccgcagc
     1021 cgttggggcc gatgatggct gtcaccttcc ctttgggcag ggctacgctg aaattatgga
     1081 ggatggtccg ccgaccgcgc cggacgacga ggtgctggac ggtaatggca tcagccatgg
     1141 ctcacacttc cttccgcagg agaaataaga aaaagggcgc ccctataaag gccatgatga
     1201 tgcctacggg caattcggcc ggtgccagga tgagccgggt cgccgtatcg ctcaaggtca
     1261 cagtcgccac gccgaagatg gccgaaccag ggatgatgta gcggtaatcg gacccgacca
     1321 ggaggcgcac gatatgtggc acgatgaggc cgacgaagcc cagcagaccg gcaacgctga
     1381 cagcgctggc agccagcagg gcggcgacgg ccgtcaggcc caggcgggcg ccttcgacag
     1441 acaggcccag gctgcgggcc atatcatcgc cgagctggag caggttgaga taataggcgc
     1501 cgacagctga taaagccagg ccgatgaccg tatacggtgc gatggccgtc acgtctttcc
     1561 agctgctggc cgccaggccg ccgaccatcc acagcagggc tccgtggacg cggtcgctgt
     1621 gaaagatgag gatgcccgaa atgatggccc ccagaaaggc cgacacggcg acgccggcca
     1681 ggatgatgcg gacaggcacg atgccgtctt tccaggccag gaggtaaatg acggcggctg
     1741 cggccatggc gccgatgaag gctacaggcg tcatccagaa gagataagcc ggccagacga
     1801 tgaggacgac gataccggcc aggccggcgc cggctgaaat gccgatgata tgcgggtcgg
     1861 ccaggggatt gcgcatgacg gcctggagga tggcaccgga caaggccagg ttagccccga
     1921 ccagggctcc tgtcagcgtc cggggcaggc ggatattcca gataatctga tccgccgtcc
     1981 gggccgacgg ctgccagagg atctgcagga catcggccgg cgtaatggcg gcaacgccct
     2041 gcatgaccga cagcaggcag cctcccaggg ccaggaggac aaagacagcc agcatgagcc
     2101 agcgccagcg ggtccgatag cagccttccc tattcatgag ccgactccgg atagacgagc
     2161 cgggccatat aggctacggc cttggggtag tccaggcccg gattgaggag gaagtatttt
     2221 tccggcagga catagacctg gcccgccttg acggccgtca gggcggccca ggccggattg
     2281 ccctggatat cttcgcggat gcgtttttca atcttatcgg acgaccccat ggaggtcagg
     2341 aaaatgacat ccgggtcggc ctggaccagg ctttccatac tgtaggggat gcgcgtcgtc
     2401 tggccgtcgc cagcggcatc ggcagcgata ttcttcaagt gcaggagatc cgccatgtcg
     2461 ccggcaatac tcgacggcaa ttcagccgat acggaactgg gtgtcacgtg gaggatggcg
     2521 acccgtttcc cctgggccgg tacggcatcg gtaacagcat ggacggcctg gtccagttcg
     2581 tcttccttgg ccttggctgc ctcttctttg ccatagacct tgccgatgac ctcaaggttg
     2641 cgcttaacgt cgtcataggt cttggtcttg aggaccagga ccgggatgtg gctctcttcc
     2701 aactgggccg ccaatttttc gtggtggtca gcgctggcaa tgactaaatc gggctggcag
     2761 gctaggacct tttccaggct cacgttgtag acaggtgcga cgtccggcag atgctgatat
     2821 ttttccggct gcgatgccgc ttcggctttg accgtcgccc ggccggcacg gtcgccgtcg
     2881 ccgg
//
LOCUS       DAWCYS010000218         2876 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1776_length_2876_cov_5.539171, whole genome shotgun sequence.
ACCESSION   DAWCYS010000218 DAWCYS010000000
VERSION     DAWCYS010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1776_length_2876_cov_5.539171"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          685 a          808 c          780 g          603 t
ORIGIN      
        1 gcgggccctt tgaagacgct gccgtcgtcc tggaaaggcg gcgccatggc taaggtctat
       61 ctcagcatcg gcgccaatct gggtgaccgc atggcgacgc ttcgccaggc cgtccgcctc
      121 ctggccgata cgccgggcgt gacggtcacg gctgtttcgc ctttttatga aacaccgccc
      181 tggggcaaga ccgaccagcc agccttcatc aatggggccg tggctgtcga aacggaaggg
      241 tccggacagg agctgctcga tgcctgtctg gccattgaac ggcgtttggg ccgtgtgcgc
      301 cgcgaaaaat ggggcgcccg gactatcgat atcgacctcg tctatagccc ggacgacgcg
      361 tgtgagacgg aaacgctgac cttgcctcac ccttatctca cacagcgggc tttcgtcctg
      421 gtccctttgc gggacttggc gccggacctc atcctttgcg gccggccgat cggcgactgg
      481 ctggaccggt tgccggatac agactctatc agaaagaagg acagtaacta tggaaaaaat
      541 actcattgca gatgatgaac agctcatgcg tcaattggtc atctactact tcagcaccga
      601 aggctatcaa atcctggaag ccgccgacgg catagaagcg ctggaaatct atgataagga
      661 acatccggac ctcatccttc tggacgtcat gatgcctggt tatgatggct ggacggtctg
      721 ccgcgaaatc cgccgcgaat cgacggtccc catcatgatg ctgacggcga aaggggaaga
      781 aatcgaccag ctcttcgcct atgacctcag tgccgatgaa tatatcacca agcccttcag
      841 tccgaagatt ttagtcgcca agatcaaggc cctcctgcgc cgttcccaga atgaacagga
      901 aacgcatgaa accgatgagg gtgtcgctat cgaccgcgat gcccgtcagg tcgtcctcga
      961 cggcaagaac gtcgacctga gcccgacgga atacaagctc ctcaactacc tcatgagcaa
     1021 taccggcaag gccctgagcc gccgccagat cctcaaccag gtatggaact acgattacta
     1081 cggcgatctg cgcaccgtcg acacgcacat caaccgcctg cgcatcaagc tcggcaataa
     1141 aggccgctat atccgcacag tccgcggcta cggttaccgc tatgatggca aatgaggaaa
     1201 gggcgctggt cgttaaaatt taaggccggc ctctatgtcg gcttttttac gattttcttc
     1261 attatcactc tcatggttgc cgtcggcttc ggctttgaac gctactatta ttccatgaag
     1321 aaagaagcta tggtcgaagc gagtcagaaa atcagcaata tctaccggaa agagggcggt
     1381 tcagatgaaa gttcgctgga cgacctgagc cagcagtatg ccttggatgt gctcatcgtc
     1441 gatcagggga aattggtcta ttcgtcccgg ccaggccggc gggtccatct gcctatggaa
     1501 aaagatatac aagatgaaga agtcatcgtc caagggaaag aaaatgaagc ggctgctgac
     1561 aagccggtct tccgcctgcc cctgcatatc gccgaactac gggatttgct cgatggcaag
     1621 atgcctgaag aagaagtgct gggcaaggtc cagctctata aggatacgcc tgatttcgag
     1681 catttcaatc tcatcgaccg cgtccaagac ggagcttacg tcattatcag ccagtccgtc
     1741 gcacccttgc gggagaatat ctacatcgtc cagcggttca tcatcgtctg cggcctcatc
     1801 tggctggtcc tggctattat cggtacgatt ctcttgaccc agcgcatgac ccagcccctg
     1861 ctagaattga agaacctggc cgtcgccatg gctcacctcg atttttccaa gcgctggaag
     1921 gggaatttca atgatgaaat cggggagctc ggcgacagta tcaatacgct ggccaaccag
     1981 ctcaacgtcg ccctgacggc tctgcagcag tccaatacgg aattgcagaa gcagctcgac
     2041 aaggccaagg aagtggaaca catgcgcaag tccttcctgt cggctgtctc ccatgagctg
     2101 aagacgcctc tggccatcat ccagggctat gccgaagggc tggacacgct ggatatcgac
     2161 aaggaaacgc agcagcgcta ctgtgccgtc atccgcagtg aaacccagaa gatggataaa
     2221 ctcgtcaagg acctgctcaa cttgtcgcgt ctggaaacgg actctttcaa actggaacag
     2281 acggtctttg atttcacggc cctggccgaa gaaagcaagg cccgctttgc caaggctctg
     2341 gctgataagc atatcctggc caagtgggaa ctgccagctg aaatgaacgt ctatggcgac
     2401 ccggaacgga tcgatacgat tctgaccaat ttcctgagca atgccatcga ctatacgccc
     2461 gtcggcaggc atatccacgt ctttgccgat gacctgggga agcgctaccg catctgtgtc
     2521 tacaatgaag gcagccatat cggcgaagcg taccagcagc gcatttggga accgttctat
     2581 aagatcgatt catcccgggc ccgcagcccg aaacgcgttt tcggcggcca cggcctgggt
     2641 ctggggaccg tcgcggccct ggtgaaactc cacggggaaa cctacggcgt ccgcaacgaa
     2701 aaagacggcg tcaccttctg gttcaccata aagaagagat agctcgtcgt tcatcgtagg
     2761 tcgttggtcg ttgtaaggga taaaaaatgg gacctcgcac gaaggttttt gccatcatgc
     2821 gaggtcccat ttgctttata gtttgtagaa gatggcttta aatttaaaac catcgg
//
LOCUS       DAWCYS010000219         2849 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1791_length_2849_cov_3.789907, whole genome shotgun sequence.
ACCESSION   DAWCYS010000219 DAWCYS010000000
VERSION     DAWCYS010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_2849_cov_3.789907"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          608 a          933 c          828 g          480 t
ORIGIN      
        1 gtcggcgccg gcgatgcacc ggatgccgaa aaattcggcg acaaaggagc cgacacgtat
       61 ggtcacatcg acgccaatgt acccttgaag gtgcctaatc tgcgccgcct gggtctgggc
      121 cgcgtcgccc atatccatgc ggaaccggcc gatgtcgtcg gctcgtatgg cctcatgcag
      181 gaaatctcgg ccggcaagga tacgaccagc ggccattggg aattcatggg caaccccgtg
      241 gaaaatccct tcccgacctt ccccgatgcc ttcccgccgg acctgttgaa agcctttacg
      301 gaaaagacgg gctacggcta catcggcaat gaaatcgcgt cgggcacgga aatcatcgaa
      361 cgcctggggc cgaaacattt ccgcacgggc ctgcccatcg tctacacgtc ggcagacagc
      421 gtcttccaga ttgccgccca caacgacgtc atccccctgg aagaattgta ccgcatctgc
      481 gacattacgc gccgcgaagt ctgtgtcggc ccttatgaag tagggcgcat catcgcccgg
      541 ccgttcatcg ggacccaggg ccatttcgtc cgcactggcg accgccgcga ttacagccgt
      601 ctgcccaagc ggaaaatggt cttctcctat ttgtccgaag cgggttatgc cgtcgtcggc
      661 gtcggtaaga tcggcgacat ctacgcccac atcggcctga ccgaatcgta ccacacggcc
      721 aacaaccacg aagacatgga agccctgcgg acccagctgg cagctcatcg ccaggataag
      781 ggcctgctca tggccaactt cgtcgacttc gacagcatgt acggccaccg ccgcaacgtc
      841 cagggctatg ccgactgcct ggaagccttc gaccaggaac tgggccagct cctgacggaa
      901 atccacgacg acgaactgct ggtcatcacg tcggaccacg gcaacgaccc gacctggcac
      961 ggcacggacc atacgcgcga acgggtaccg ctcctgctct acagcccggc cctgaaggaa
     1021 tcggtcgact tagggctccg ccagacctat gccgacctgg gcatgagcat catggataat
     1081 ttcggcctga caggccttga attcggtacc agctttttat cggaactgag gtgatcctat
     1141 gtggcccatc gactgcatcg aacacaaacg ggacggcaag gtcctgacga aagaagaaat
     1201 ctcgcgcttc atcactgact atacggcagg gcgggtcgcc gactaccagg cggcggcatg
     1261 gctcatggcc gtctatttcc agggcatgac ttatgaagaa acgaaggaac tgaccctggc
     1321 gatggcccat tccggcgata tggtcgactt gtcgtccatt ccgggcatca aagtcgacaa
     1381 acacagcacc ggcggcattg ccgatacgac gacgctcatc gtcgcgccct tagtggcggc
     1441 agccggtgtc cccgtcgcca agatgagcgg ccgcggcctg ggcttcacag gcggcacggc
     1501 agataaactg gaatccatcc ccggcttcca cgtcgtcctg ccggaagaac agttcctgga
     1561 acaagtgcgc cgcatcggcc tgtcccttat cacccagtcc ggcgaaatcg caccggctga
     1621 taagctgctc tacgccctgc gcgacgcgac ggcgacggta gagagcatcc ctctcatcgc
     1681 cagctccatc atgagcaaga aaatcgcctc tggtgccgat gccatcgtcc tcgacgtcaa
     1741 atacggcgac ggcgccttca tgaagaccca agaacgggcg cgggaactgg cccggaccat
     1801 ggtcggcatc ggcaacctcg ccggccggcc gacacgggcc gtcgtcacgt ccatggaagc
     1861 gcctctgggc acggctatcg gcaactgcct ggaagtcgat gaagccgtcg aagccctgtc
     1921 gggcaaaggc ggccggcggc tcatggaagt cgtcgaagcc atcggcgccc agatgctcat
     1981 cgtcggcggc aaggcccagg acgaaaggca ggggagagcc atgatccgcg accttgtcgc
     2041 ttccggcaag ggcctggcta agttccggga attcgtcaag gcccagggcg gcagtacgtc
     2101 gtggatcggc aagcggcccc tcaccaaggc gccccaggtc tttacggccg tctgcaccga
     2161 tgaaggctac atcacccgca tcgacggccg ggccctcggc gaaatcgcca tggctatggg
     2221 cgccggccgg gcccgcaagg aagatgccat cgaccccatg gtcggcatcc gcctgttcaa
     2281 agagctctat gacccggtcc gtcccggcga agccctgttc accttatatg gcaaggaagg
     2341 cgccgatatg atggccctgg cccggcaggt cgccgaccac atcatcgtca cagccgaaca
     2401 ggctcccgtc caggaaccgg tcatcagcga aatcatcgtc taaatagaaa ggaatcccat
     2461 gaaagaaaca cggcaaatcc ccgtccagaa ggggaaaacc tatgatatat ccatcgtccg
     2521 cctgggcagc agcggtgaag gcgtcggacg ctatgaaggc tttacggtct tcgtgccctt
     2581 cgccctgccc agtgaaaccg tgcgggcccg cattaccctg gtcaagaaga cttacgccac
     2641 ggcttccctg gtcgaagtcc tgaagccctc gccggaccgg attcagcccc agtgtcccgt
     2701 ctacgaccgc tgcggcggct gccagctcca gcacctgagc tatgaagggg aattgaagga
     2761 aaagcgccag caggtccggg atgcgctgga acgcatcggt catctgcagg gcctcgacgt
     2821 cctgccgagc cagggggccc atacgccct
//
LOCUS       DAWCYS010000220         2838 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1805_length_2838_cov_4.064319, whole genome shotgun sequence.
ACCESSION   DAWCYS010000220 DAWCYS010000000
VERSION     DAWCYS010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_2838_cov_4.064319"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          652 a          837 c          767 g          582 t
ORIGIN      
        1 cccctggata gaggacatcc agctgcccat cgtcggcatc ggcggtacag cccgtaactt
       61 cgccaaaatg gaccagcggg cgacgaatta tgaactgtcg aagctccata attacatcat
      121 gcccctggaa cattttgaaa atctctatca tgaaatcacg acgcggacca gtgccaaccg
      181 caagaaaatc gacggcctgt ccagtgaacg ctctgacctc atcgtcgccg gtgcagccgt
      241 catcaagacc atcttcgaca tgacgggcag cccggaaatg gtcgtcagcg gctgtggcct
      301 ccgcgaaggt ttattctttg aatactacgc ttcgtactgc cagctgccgt caccgcgttt
      361 cgacgacatc ctcgatttca gcgtcaagaa cttcatcggt acgctgagcg gcatcatcat
      421 caaccaggct cactacgacc aggtcacgcg cattgccacc cagctcttcg accagctcca
      481 gccgctccac ggctacggcc cgcgggaacg cctcctgctg cagacagccg cccgcatgca
      541 cgatgtcggc aagatcatca acttctacaa ccacgcccgc cattcggcct tcatgatcgc
      601 tcatgccccg ctctacggcc tgacccaggt cgaacaaatc attacgggct tcatcgccgg
      661 cttccatcac ggcatcagcc gcaagatcat ccgggcctac cgttacgcca acatggcctc
      721 tgccgaagac tggatcatga tccacaaact ctcgaccatc ctggccctgg ccgaagcctc
      781 ggacctgacg tatgaacaaa tcgtcaccga cgtcgaagtc acactggccg aaaacgtcgc
      841 cgtcatgatc atcacgacgg cgacgggctc gacgtacaac gccgctgact acgaaatgaa
      901 acaactgagc aaacaattca aaaaagaatt cggcgcttcc ctgctcctcg tctggaagta
      961 agggtccgga taaaaaaagg ggctgtcgca catgcgacag ccccttttta ctcttatcgc
     1021 cgcatcatgt cttcagccgt gacgtcggga tggtggtact ggtcttcaga gaggatctgt
     1081 ttaccatgga cggtgacggc ttgctgggga caccagtgga cacaggccag gcaatggctg
     1141 caatgagagc cgatgacggc ccctttcgcc gtacagatga tattgctcac cggacagatg
     1201 cgggcacaga ggccgcaggc aacgcagcgt cccccatcga cagctggcgt cagccagcgt
     1261 tccccagcat ggcggatcag gcccaggttc atgagccagg ccgacagcga cgaatggatg
     1321 gccgtcgtat cccggacctg cccgcgtatc ttatcggctg cggcagcgac cagttcatcg
     1381 gcgtggtcca ggcggttcat atcgtagcgg atatggcgcc gcgacgcgat agtactgttg
     1441 gcgatcatcg gcaggacagc cccgtaggac aaagaacaat gttgacgccg gaggatccgg
     1501 tccatcgtcc tgagcgaccg gccaggcgca ctgccgcaag tagcaatgcc ataaaaatag
     1561 gtcgatttct ggaaacggac ctgtctgatg aaatcctgta tgggttcggg aagatccccg
     1621 aaatagacgg gaaagacaaa gccgatgatg cgatcctgga tacgctggca ccgttcatgg
     1681 atagccgaaa tcggcacggc cgtatcacca gtcagttcag ccagcttctt ggccacatat
     1741 aatgaatttc ccgttcctga aaagtaaaga atcataaggc tcacctcaga gaccgcactg
     1801 ccagtttccc gcttgcaggc ggacggtatt ttacatccat tatatcatga aaccctgtca
     1861 tttgtcttga tacatttgtc tttacacgta accgcgctcg caaaggctga cgaaggtccc
     1921 gtcgccgatg atgatgtggt cgaggacggg tatttccatg atctggccgg cgtcggcaat
     1981 gcgctgggtc acgacgatat cctcgtggct cggttcgggg ttgcctgacg gatggttatg
     2041 gaccaggacg acagctgcgg cattgtagcg cagggcatag cggaagactt cccgcgactt
     2101 ggccaggctg gcattgatcg tgccgatggt cagcgtgcga taggcgatga gctggttctt
     2161 cgtcgataaa tagacggcgc cgaactgctc ttgcggcaaa aagcgcagat cttccatgac
     2221 atagccggca atggcctggg gcgtactgaa gtcaggcgcc gcttccttta cgtgctgccg
     2281 ggaaatacgc cgtcccagct cgacggcggc acagaccgtg acggccttat ccgtcccgat
     2341 tccgggaatc tgacagaggt cgccgatgct ggtatcactg agataacaaa tattttcccc
     2401 tggcagggaa tgaaggacct gccgcgacac atcgaggacg gattgtccct tcgtacccgt
     2461 ccgcaataga atcgccagca aatcctggtc ggtcacagcc gaaggaccgt gacggaggaa
     2521 tttttcccgc ggtttctcac aaacagcgag gctgcggaaa gggtcgctca tacatggacc
     2581 ttggctttgt ggaacaactt gcatacctgg gacaagggca ggccgacgac gttggtatag
     2641 gaaccattga tcttatcgac gaaagccgca gcccggccct gtatgccata ggcaccggct
     2701 ttgtccagcg gttccccaga agcgacatag gctttgattt ccttttcact cagtcgtcgg
     2761 aaccagactt tcgtttcgac gaccttcgtg tattccttat ggccattgat caaagcgatg
     2821 cccgtataga cggagtgt
//
LOCUS       DAWCYS010000221         2820 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1812_length_2820_cov_6.070163, whole genome shotgun sequence.
ACCESSION   DAWCYS010000221 DAWCYS010000000
VERSION     DAWCYS010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_2820_cov_6.070163"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          589 a          892 c          810 g          529 t
ORIGIN      
        1 gccttatacg ctgctctgcg atggccaacc tgtcatgaaa cacgaggcct atgtcctcag
       61 cctgaaggat ttgaaatccc tcgattatat cgacgacttg ctcgatgccg gcgtcacgtc
      121 ctttaaaatc gaaggccgca tgaagggcga tacgtacgtc cggaccgtcg tttcggcgta
      181 ccgcagggtc atcgatgccc attaccagag cccgcagcag cgcaaggccg cccatcgcga
      241 agcggaagcc cttctggaag gcgctttcaa ccgcagttat caggccgatt tcctgggaga
      301 ttccgtcagc cgccgcacgt tgaccgaacg caatccatcg gggcggcaga cggctgatga
      361 catggccggc ggattgacgc ggaaactttc catctatggc cacgtcgaca cggaagacca
      421 ggggaacctg cgcctgacct tgtgggatga aaatggccat actgcctcgg ctgtagccga
      481 tttccagccc gaaccggccc gccagcggcc ggcaacggcc gattacgtcc agggccagct
      541 cggccgcctg ggcgatacgc cctttgccct ggccagtgtg accgtctggg atgaaacgcg
      601 catgatcccg gccagcgtcc tcaacggcct gcgccgtcag gccgtccagg ctttgaccga
      661 ggccatcctg gcggattacc cgcggccggc agccggaaag gcccggcagc ggcaggctcc
      721 ggacaggcag cctggggaca aggaagccat gcagctcgtc gtccgctgtg acagtgtcga
      781 cggcgtcgtc gccgccgcgg accagggtgc cgacgtcatc atctttggcg gcgaatcgta
      841 ccatcatcat cccttcggca ccaaggcctg gcaggccgcc gtccaggccg ctcatgaaca
      901 gggcgcggcg ctgtgggccg gtacgccgcg tattgtccgc gaagagaacc ggctggtcgt
      961 ccagaacgaa ctggaacggg ccatcacggc cggcgtcgac ggcatctatg ccggtgccat
     1021 ggccattttc gccatgatag ccgacttgaa tattaccgta cccgtccggg ccgattggtc
     1081 cctcaatatc tttaacagcc aggcggcccg ggcctatgca tccttgggct gtacggccat
     1141 tacggcgtct gtcgaggcga cgttgcgcca gttgaaagaa atggtccgcc agagcggctg
     1201 tcccatcgaa gccggcgtcc agggccgggc cgaaatgatc gtcactgagt cctgcgtcat
     1261 ttcggactgt gccggcaagg gccagaagaa aggctgcccg ggcgtctgca accgcggtgc
     1321 ctatgccctg cgggaccgcc gggacgaaac gttccctgtc gtcaccgacc agtattgccg
     1381 caaccacatc ctcaacagcc gggatctgga tatggcgccg tattataaag acctgtgccg
     1441 cgccggcatc gccgccatcc gcatcgaagg ccgcggccgc acgccacagt ggatcggcca
     1501 gcaggtcagc cggtaccgcc gcctctgtga cggtacggaa acgatgctct taagcaggga
     1561 agaccagacc gtgacgcgcg gtcatttctt ccacggaatc ttataacaac aggagtaatg
     1621 ccatttatga ataaaagaag tatgaggatt ttgggattct accaggtcca gaacatgctg
     1681 gtccgcctgg ccccgtcccg gttgtctaaa gaaatcgccc gccgcctgag accggtgaac
     1741 gatggcgatg tcattgaaaa gaacctgacc gatacggaag aagccgtccg ctgcatgcag
     1801 acggaaacga gcacgccctt cggcggcatc gtcgatatcc gtccgtccct ggaaaaggcc
     1861 aagcgcgaag tgaccctcga ttcccatgaa tgtatcgata tctggaataa cctccagcat
     1921 tatggggaaa tccggtcgtt cttcgccgaa cggggggctg agtatcccca gctggccgaa
     1981 caggcagagg tcatcggcga tttttcccag ctttctcatt ctttcgccac tgtctttgac
     2041 aacaaccgcc agatccgcga caatgcgtcg ccggaattga tgcgcctgcg cagccgtatc
     2101 gtcgaactgg aacggcagac caagcgctat gtcaataatg tcctgcagaa caaggaatac
     2161 cagaagtatt tccaggatgc cctggtcaca gtgcgcaaca accgctatgt catccccatc
     2221 aagcaggaat accgccatgc ctttcccggc atcgtccacg atacgtcggc cagtggttcg
     2281 acgctctatg tcgaaccgct ggccatcgtc aatgccaaca acgacctgca ggccgcccgc
     2341 attggcgaaa cgaaggaaat cgaacgcatt ttccgccgcc tgacggccaa agtgcagcag
     2401 cagtacaatg acttgtatga cagcacgaag tgtgtcggtc agctggaatt cgcctttgcc
     2461 aaggcccagt tggccttgaa gatgaaagcc tgccgtccgg ctatttccaa gacgcgggaa
     2521 atcaggctca tccaggcccg ccatcccctc atcgacgcca agcacgtcgt gcccaatacc
     2581 atcgtcatcg gcggcgatta ccgcatcctg ctcattacgg gctccaacac gggtggcaag
     2641 accgtttcca tgaagaccct gggccttttg gtcctcatgc atcaggccgg cctgttcatc
     2701 ccagtcggcg aatcgtcgga actgcccgtc ttccgcgacg tgtttgccgc actcggagac
     2761 ggccaggggc ttgccccgga ccggagtacc ttttcaaggc ctctggagca gttctgctcg
//
LOCUS       DAWCYS010000222         2818 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1814_length_2818_cov_4.513934, whole genome shotgun sequence.
ACCESSION   DAWCYS010000222 DAWCYS010000000
VERSION     DAWCYS010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_2818_cov_4.513934"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    273..282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          690 a          713 c          702 g          703 t
ORIGIN      
        1 acctgcccga tgccctggcc gtctattcca gcctgcggga catctccatg gacagccgga
       61 ccgtcgtcat gggcgaagtc ggcctcaccg gcgatatccg ccgcgtcccc caggccctgc
      121 gccgcgtcaa agaagcggcc aagatgggct tcaacgcctt tatcctgccc aaaggcaacc
      181 tggaagacat cgaaacagac aaattcccgg actgccattt cgtaggcgta gcgcccctgc
      241 gcgaagccct cgaaacggcc ttccgaagat aannnnnnnn nnctgtcttt ctgcgtcagc
      301 gcctgttcgc tggccttttt tgagtaaacc tgttacgcta attttagtgg ctagtggcta
      361 gtggctagtg gctagtggct agtggctagt gctcccctga tagggaatct gtcagcgcag
      421 ctgactgaga ggttttcaag gcaacctctc cggccttacg gccacctccc ctattatggg
      481 aggcataatc cgcatgggcc gtctcaagcc attctggccc atcaggtgcc ggccgatgaa
      541 gggcgcaaga cggttgtcag aacggacagc tgtagctggc ggcccgcctg acccgtaggg
      601 ggcgcccacg ttgggtggct gttttccggc tcccctgata ggggagctgt cagcgaagct
      661 gactgagagg ttgatggctg gctgttaacg ataactataa ccggaaagaa gtatatcatc
      721 atgcgaaaat ggattcagtt agctttactg gttctctgct ttttctgtct cagcggagct
      781 gccctggctg cctcggctgt gacgccggac ggctatttca acggcatccg tctggccggc
      841 aaagtccgcg tcgtcgattc cttcccggat atccgcgtcc aggtcgtcag cgccttcccg
      901 gacctgcgcg tccagactgt cgactatttt cccgatgaca tcggccaatg gcagttcgtc
      961 gattacggcg aagacttcac agtccaattc gtcgagtcct tcccggacat ccgcatccag
     1021 tttgtcaaca gtttcccggg agtcccctaa cttagtgaag aggttatccc catgaaaata
     1081 cgcgcgttac agttggtagg ccacaaaaat acacggccag cggctttgtg cgtagggacc
     1141 gtcccaagcc tttttggccc gtcaggtgcc gaccgacgga aggcgcagga cggttgccaa
     1201 aacggacagc tgtggctggg cggcccgctg tgctattcgg ccacagccta tgcacgtata
     1261 atgacaggtt ttttattaga aagcaggtga caaaagtgat ttgcaaaatg cctataggaa
     1321 tagatgattt tgggaaaatc cgcaataaag atttctattt tgtggataag acagcattta
     1381 ttcgccagct catcgatagc ccccgggaag tcactttgat tacgcggccg cgcagctttg
     1441 gcaagacgtt gacaatgagt atgctggaat atttcttttc cattgaaaaa gaaagccaga
     1501 gccgtcattt attcgaccat ctggccattg aacaagccgg gcccgaatat atggctcagc
     1561 gtggccagta tcctgtcatt ttccttacac ttaaagatat ccaaaatccg tcttggacga
     1621 catgggaacg gatgctttcc tttatacggc tgtttttagc acaagtctat gatagatatc
     1681 gctacatccg tgaaaaggca gatatcaatg aagtccaagc ccgccttttt gatcacgtcg
     1741 tccaaggaca ggcgacagaa gacgaattgg ccttatcttt gaccaatttg atggctatgg
     1801 tacagagtta ctatggccga aaagttattc tccttatcga tgaatacgat gcaccgattc
     1861 aacaggcttg ggaaagcgga tattataccg gttgtatcgg tttcatgcgc caatttttga
     1921 gcagcgcctt gaaaacgaac aacaccttgg aatttgctgt cctgacgggc attacccgca
     1981 tctctaaaga aagcattttc agtggcctga ataatctgga tgtctgttct gttttgcgcg
     2041 atcagtacgc cgaggtcttc ggatttacgg aagcagaaat acaagaactc ttagaaaccg
     2101 tacatcatgg ggaaaagctt ccagaattaa aaaaatggta tgatggctat cacattggct
     2161 gcgcagaaat ctataatccc tggtccgtca tcaattatgt ggcccatgac tgccaagcca
     2221 ggccttattg ggtccggaca tcgggaaaca gccttttacg tcatctcctt tctcatacgg
     2281 atgagttacg gattcagatg atacaaaatc tgctgcatgg caaagccgtt cgcgtcacct
     2341 tagatgacag cctcatctat tctgaaatag accaggaccc gggagccctg ttcaatctcc
     2401 tgttgacgac gggctatttg actgtcgaaa aggtcttatc ccttagcgat gaccgttatg
     2461 cattaaggat acagcatgag gaaatccgca aactatacag cacagaaatc atgaatgccg
     2521 taggaaacca tgtgaccaag aacacctttg atgacctgtt cgattgagct aacgaagcct
     2581 tacgacaaat tgaaaccagg aatacatcac agagtttcaa accaggggaa tccataacgt
     2641 atggaaatac gggattgctt tttatcacag tttcgccaga agactctcct cttaggtagg
     2701 ggcatcaatg gtgatggatt ttgcaggttt gatgcctgca aaatccactc tatgtctttt
     2761 ccgtcttagc cttttcactt cgtctacggt ataatggtag ataacagagg aggtgaat
//
LOCUS       DAWCYS010000223         2812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1816_length_2812_cov_3.927457, whole genome shotgun sequence.
ACCESSION   DAWCYS010000223 DAWCYS010000000
VERSION     DAWCYS010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_2812_cov_3.927457"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          465 a          767 c          893 g          687 t
ORIGIN      
        1 attgtttctt tggctttatc atcgggttcc gtgtgttttt cggcattgcg cgtttcctga
       61 taatgttcca gctggcgttt gacccgctgc agggcattgt cgatggactt gacgtgccgg
      121 tgcagctgac gggacatctc gttgtaggat ttgccgtcga gataggctgt caggacctgc
      181 cattccagat cgctgaggat ctcattcatc ttcagttcga tatcattgaa ttcttcgcgg
      241 ctgatgacca gctcttcggg atcgctgacg cggatgccgg acaggatgtc ctgcagcgtc
      301 cggtccgatt cgtcttcata gacgggcttg ttgagagaca catacgagtt aaggggtatg
      361 tgtttctgcc gcgtcgccgt cttgatggcc gtgatgatct gccgggtaat gcataactcg
      421 gcaaaggccc ggaacgacga cagcttgtcg ccgcggaaat cccgcgtcgc cttgtagagg
      481 ccgatcatgc cttcctggat gatgtcatcc cggtcggcgc cgaccaggaa gtaggcgcgg
      541 gctttggatt tgacgaagtt gcgatacttg ttgacgatat aatccgccgc tttgccatcg
      601 tgctcttcct gggcgatgcg tacgatttct tcatctgtca tgcctgcaaa gcggtcatgt
      661 ttttcttctg cattggacgc tgcctgcata atcgttacct cgcttatttt aaattattcg
      721 cctgcctgcc gctgccgcac gacttcatac atgagcaggg ccgctgcgac ggaggcgttg
      781 agggagttga cgtggccgaa catggggatc tggaccagga cgtcgcacgt ctccttgacc
      841 aggtgcgaga tgccccggcc ttcactgccg atgaccagga cgaccgggtc cgtcagggag
      901 ctgtggaaat agtccgtctc gccgtccata tcggcgccga tgacccagaa accgcgcttc
      961 ttgagctgct tcagggtctg ggcgatgttg ccgatctgca cgaggggcat gtattcgacg
     1021 gcgccggccg acgtcttgcc gacagtctca ttgatgagcg ccgtccgccg cttgggcagg
     1081 aggacggcgt cgacgccggc acattccgcc gtgcggatga tggcccccat gttgtggacg
     1141 tcttcgatgc cgtcgaggag gaccaggaac ggtaccttcc ccttttcatc agcagcgatg
     1201 agaacgtctg tcaaatcggc gtattccacg ggcttggcaa aggcgatgac gccctggtgg
     1261 cggttccccg gtgccaggtg gttgaggacc ttcaggggca cgtcttcgac gaggatttcc
     1321 ttccggcctg ccaggtcgcg gatgcgctgc agcggctgcg actcattgcc ggcagcgacg
     1381 tacagcttgg tgatggaccg gccggccttc agggcttcgg tgacgctgtt gcggccgatg
     1441 atgatatttg cctcactcat tccattcctc catgtttttc gttacaatag gctttcgttt
     1501 cttcatagac ctgggccatg acggcgtcca ggcgttccgt ccggccagcc atgaccaggt
     1561 agcccaccag ggcttccagg gccgtgctgt cgtggtactc ggcgacggtg gcgctcttcg
     1621 gcgccagggt ataggcattg cgggcccgct ggcagacggc cttttcgtcg tccgtcaggc
     1681 cgtcgtggat gcggcggtag acataggcct gggccttggc ggatacgaat tctgccgcca
     1741 gcgtgtgcag gacctggacg tgggggatgc ccgtttccac caggcgccgg cgcagggtca
     1801 gggcgtaata ggcgtcaccg acgaaggcca ggttgatcga gtcgagatgg gccgcatcct
     1861 ggtcgggcac gtccaggcgc ttgccgtcgg caaaggcctg gtaggcccgt ttcttcagtg
     1921 cttgcagttg tttgaatttc actttttcca gcgcgctccc tgcggcgaat cttcgatagt
     1981 gatgccaaga gcggccagtt tatcgcggat ggcgtcggcc agggcgtact gtttctgggc
     2041 gcggcattcc tgacggatgt cgaggatggc ctgcatgagg tcgtcgtagt ccttgctgct
     2101 ggcgtcttcc ttcttgccgg tccagcggtc ttcgaggatg ccgaggatat ccgtgaaggt
     2161 cttgaagacg cgcttgactt ccgacatggc tgccgaatcg acggggacgc cgttgttgac
     2221 gacggcattc ttgaagatgt tgacgttctt ggccaaatcg aacatggccg cgctggccag
     2281 ggccgtattg aagtcgtcgc acatagcatt ttcaaagtct tcttcgtcct tgtgggccgt
     2341 ttccaggagc tgctgggctt ccggcgattt agcaccggcc ggcatagcca ggagttccag
     2401 gacgttgtcg atggccgtgc ccaggcgttc gaggctgcgc gtcatttctt ccagccgttc
     2461 gtcgctgaag tccaaggggc tgcggtagtg gttggaaatg aggaagaagc gcagggcatc
     2521 ggggctgtag tgttccagga cgtccttgac caggaagaag ttattcaggg acttgctcat
     2581 cttttccgag ttgatggtga tgaagccgtt gtggagccag tatttgacca tcggtttctt
     2641 gcccgtaaag gcttcggact gggcgatttc gttttcatgg tgcgggaaga tgaggtcgct
     2701 gccgcagccg tggaagtcga aggtatcgcc aaggtactta ttgctcatag ccgagcattc
     2761 gatgtgccag cccggacggc ctttgcccca ggggctgtcc cacgacggtt cg
//
LOCUS       DAWCYS010000224         2805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1821_length_2805_cov_6.109091, whole genome shotgun sequence.
ACCESSION   DAWCYS010000224 DAWCYS010000000
VERSION     DAWCYS010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1821_length_2805_cov_6.109091"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          544 a          829 c          857 g          575 t
ORIGIN      
        1 cgccggcaat cttatagatg tcgtggagca cggcgccggt cttcgacgtc aggacgccga
       61 tgcgccaggg gaagcgcggc agctccttct tgtggctgct gtcgaaatag ccctgagccg
      121 ccagttcatt cttgatgcgt tcgtactggg cctgcagaga ccctgtcccc tgggaaaaga
      181 gctgttcaat gataaaggaa agggcgccgc ctttttcata gacgttgacg gcgccgatga
      241 cgacgacatg gtccccttcg cggacggccc ggaccgctgc cgtcacgcgg ctgcggaaga
      301 cgatggcccg gatggagcac tgatcatcgc ggatattcat ataataatgg ccgttcgacg
      361 cccgcttgag gccgatgatg ttgccttcca tagcgatgct ctgcaagcgg taatcgccgt
      421 ggatatcatc tttgatgcgg cggacgacgt ccgtaaccga tgcatatttc atgatttctt
      481 cccttcctgt acggcagccc agaaggcggc gcccgacgcg ttgtcggcac tgaagccgtc
      541 ggctgccaga ttcaggtcta tctgatggcg gcggcaataa tcgctcatct gccggcgcag
      601 gtagccattg gacatgacgc cgccagcggc gacgagatgt gtcatgccat cggcagcggc
      661 gtaatcgagg aggctctcca agccgcgcca gaccgtcgac agcgtccagc tgcaaaggcg
      721 ggccgggtct tccccaccgg cggcgatgcg ccgctggccc tgcgattcgg gcccggaaaa
      781 gctgacctgg ccatcgcggc aaaagacatg agcttccttg acgggcggat ttaccgtctg
      841 agccagcttg tcgacatgga cgccagccgg aaagggcagg tccagggcga cgcccatgcg
      901 ggcgatgaac tggccggcac tgatgtcgct ggtgccgccg atacgggtga tatcgaggcc
      961 tttttcatcg ggcacggccc gcagcaggtc cgtcgtcccg ccggacaggt ggatgccgta
     1021 gaacggctcg ctgccgatat agcccatgct gcgcaggacg gcgaggagat ggttttcctg
     1081 atgactcagg cggtacaagg gcacgcccag aacggcggcc agggcccggc cataaccgag
     1141 gccggccagg aaagctggca tgtacgaatc accgcggcgc cggggctggt cagtcacggc
     1201 aatggcccgg atatgggcac tggccaggac gccagccagc cgttcaaaga ggaccggcag
     1261 gttgcgtgta tgctgataga ccatgttgga ctgggacagg ccgcggccgc ccttggcgac
     1321 gctcaggatg atgcgttcgt cggcgatgac gtcataagcc gcgtcgacga ggcacagcga
     1381 cgtcgtataa cagctggtat cgatgccgag aaagacgtcc atcaggcctg ggaccttaca
     1441 taagcgccca aaataccgtt gacgaaccgc gccgacttgt cgccgctgaa ttccttggcc
     1501 aggatgaccg cttcattgag gacgatggac ggcgccaggc tttccttggg gtatacgagt
     1561 tcgcacaagg ccaggcgcag tatattgcgg tccgtgcggt tcatccgttc gatgtcccag
     1621 cccttggcaa aggagcggat gatcgtatcc agttcgtccc ggtgttccgt cgtcgtttcg
     1681 acgagatacg tgcaatagtc attgcgttcc tgatcactca gttccgggcc gtcttcggtc
     1741 aaggcgtcgg tcgtgacgta atcattttcc aggatttcct gttcgctgta ctggatatca
     1801 ttttctccgt ggaattcacg ggaatataaa agttcgagcg cctgctggcg cgctttatgt
     1861 ctgctcacga gtttcctcct gtaataactt tattcagccg gcagtaccgc caggtcttca
     1921 atggtaacgg aaatatcaag gccctctggt tcgaccatgg cgtcgatggc ctcagccgcc
     1981 cgctgctgga catccagggc caggccgtgc aggtcgctgc cataacgggc gcggacggcc
     2041 agatggagat ggacgacgcc gtccgtcacg gccgcgctgc ggctgcgtat agcgacgacg
     2101 ccagaggcct ggcggccggc cgtctcggcg atatggccca gggccttggc actgacggca
     2161 taggcaaaat cctgttcctc atccatggcg gtcctcctct ccaaacacta actgttgtac
     2221 aatgatgtgg accgccgtaa ccgtgcaggt cgtcgccttt tcgacggcat ccttgacgga
     2281 cttctgcact tccagggcta cgtcgggaat acggcagcca tagcggacga ccaggcggac
     2341 cgtcaattcg atgcgccggc cgtcgataga gacgtcgacg ccgcggcccg gaagggtacg
     2401 ccccagcttt tccgctgcca cttcggcgat attcgtcccc aaatcagcga cataggccga
     2461 ttgggccgcc gtcagagccg caatagaggc aatgacgcgc tgggaaatat ggatactccc
     2521 ccattcattt acttcagtcg tttccatggg aatcacccct ttagtccaca aaaggggctg
     2581 tcgcacgtgc gaagcccctt tgatacccta tgctagcggc tggcggctaa cgacttacgt
     2641 taaccttatg ctctgccgat atactggccg gttctcgtgt cgatctggag aacttcgcct
     2701 tcattgataa agagcggtac tttgacgaca tagcccgttt ccaatttagc cggtttgctg
     2761 ccgcctgtag ctgtatcacc gcggaaaccg ggatgggttg cgagg
//
LOCUS       DAWCYS010000225         2799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1827_length_2799_cov_3.903790, whole genome shotgun sequence.
ACCESSION   DAWCYS010000225 DAWCYS010000000
VERSION     DAWCYS010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1827_length_2799_cov_3.903790"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          693 a          714 c          830 g          562 t
ORIGIN      
        1 gcctgggaca gcatcacgtc tgctgtcgat gcgggtgtcc agtggtgcat tgataagtgg
       61 gaaggactga aagaggcctt ttctcatccg attgatgccg tcgtcaattt catcaaaggc
      121 ggcgacagcg atgccgcttc cgcagcgggc cagtccgcaa gcggcggcgt tttcagtcat
      181 ccctatgtga cgtgggtagc ggaagcagga tatccggaag tcatcgtacc tatcactcac
      241 gatgcaaacg cctacaattt atgggctacg gccgggcaaa tgctcggcat cgggcctgcg
      301 gcgatgccga cgatgaccgg agtacccgcg gcggcgcctt ccggcaattc ggcacagatt
      361 acctttgcgc cgacgattaa cgtccagggc gcaggcccag ggacggaaca gcggatttct
      421 cagctcatgg accagaaaat gcgcgagttt gacagcatga tgaagcgatg ggcggcaaat
      481 cagaggaggt tgagctatga gtagcaccta cagcaccgta caaggcgaca tgtgggacct
      541 gatatcctat cgcgtgtacg gtaccgaaaa gtacgtcaaa aacctgctgg aagcgaatcc
      601 gcaatatagg aatgtcgtcg tcttccccgc agggttggcg ctcatctgcc cggatatccc
      661 gtccagcgta tcgtctattt tgcctccgtg gaagcggtga tctaattggc attactgaaa
      721 aaactacaag cggacctcaa gaaaatcgag gcccaggcca tggcgggcgt cgttgctcaa
      781 gagcatctgg ggcgccgcgc atggctgcaa gtgacctatg ataacaagga catctccgag
      841 gcactggccg aatacctgat cagcgccagc tacaccgaca atctatccgg gcaggtagac
      901 gacatctcgc tgacattgga agacaaagcg ggattttggc agggcgattg gatgccgacg
      961 aaaggcgcta cgctcgatgt cactttatgc acgtataact ggataggcct gtacgacggg
     1021 gaagccgatg tcacgttggg caaattcgag gttgatgaaa tcgagctgtc cagcgcgcct
     1081 gatgtcgtca caattaaggc cgtggccagc atggtcagtg atgacagcac acttaggagc
     1141 acgctgcgct cgcggacctg ggaaaatgtc acggtccaaa aagtagcgaa tgacattgcc
     1201 ctggaaaaca acatgaacat ttgctgggac tgcggcgatg acccggccat cgataaagtc
     1261 gagcagaacg acgaatcaga cctggacgtc ttgaagaagg tttgtgatga cgcgggtttt
     1321 gccctcaaag tcacgaccga cacgatcatc gttttcgacg aggcgctgta tgaggaagcc
     1381 gagccggtag tagaaatcta ccatcctgac acccaggaga ttatcaccgt cgcggaagcc
     1441 gacgaggacc atacaccgga gcgaatccta cacagtacgg gctattcgtt tacgtctaaa
     1501 atccgcgacg tctacaagca atgccatgta aagtacgccc aggacaagga caaatccgtc
     1561 atcgagtcca cgttggtgga gccgactaaa gaagacggtg catcgctggc actccatcag
     1621 catgtttcga agagagcgga agcggaccgc ctggccaaga agaagctgcg cgagaaaaac
     1681 agcgaggaat gcacaggcag ctttaacagt gagggccagc cgtttttatg cgccggtgaa
     1741 accgtcgaga tgattggatt cggcacgttt tccgggaaat atatcattac gcaggccaag
     1801 cacgatttaa gcagcagcgg ctatacaacg agcatcgatg tgaggaggtg cctgattggc
     1861 tactgatatt tataaggtat tggcgcggtg catccgcgtc gggcgcgtat cctcggtaga
     1921 cacggcggac gcttccgtcc gggtgacatt tccagaccat gacaacattg tatccgcgcc
     1981 gctcaaggtt ctcatgcggg gctgtaagag cacgaaagac ttctggatgc ccgccgtcga
     2041 tgaccaagtc ctttgtcttt ttgtcgccga cagcggcggc aagggaagcg gcgccggata
     2101 tgtcctgggg acgatttaca gcactgtcga tacaccgccg ggcggcgggt cccgtgtgct
     2161 gaatgtcccg gatgatttgg tgatcaactg cggcagcctc aaggtcaatg ccggcggcgg
     2221 ggatgtcacg gtcaacggca tatcccttgt cagtcacgta cacggcgggg tatcgtcggg
     2281 cggcagtaat acaggcaagc cgcagtaaag aggtgatacg gcatgtatat cgggtacttt
     2341 ggtaccgttg tatttggcgt ggctgagcat tatctagtca ctcctgatga cgtcgagcgc
     2401 tccggggagg cccggtggca gacacatgac gtcattttaa aaaagcccgt gccgcagttc
     2461 atcggcccgg gacaggagga actgtctttt aagctccatc tcatgaccct gtacaatgcc
     2521 atgccgtcgc agcagctggc aaagctccgg gaaatgaggg atacgggcat cgtctgcccg
     2581 ctcatcatcg gcggtatgcc ggtatctcaa aattactggt atatcgagag cattgacgag
     2641 gcggacgctg tctacaatgc ctacggcaag ataatcgcta tcacggcgaa cgtcaaacta
     2701 aaggaatacg acacgacgaa tacagatgaa gagtcgacac tgaatcaagt cggcaggatg
     2761 tataacggga ttacgaatgt agtcgggaga ggtggagtg
//
LOCUS       DAWCYS010000226         2783 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1841_length_2783_cov_4.792889, whole genome shotgun sequence.
ACCESSION   DAWCYS010000226 DAWCYS010000000
VERSION     DAWCYS010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_2783_cov_4.792889"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          732 a          747 c          765 g          539 t
ORIGIN      
        1 ttgaagacga atgatgacgg acagcgatta taaaatcgcg gaaaccctgc cggcgtcgct
       61 tgcacttgaa cctatcccgg accttgcgcg cgtcgtcgac gatgaactgg ccggcatcaa
      121 tcccgatttg ctcttgattt ctccggctat cgattcgctg ggcagcgcct tagttgatta
      181 tctggccgag cagatgcacg tcgacgaata cgacagcacg gcagcgctgg acgtcaggcg
      241 ccagcaggtc aaagaatctt tcctgctcca taaatttaaa ggcacgaaat gggcggtaca
      301 gcgcgccgtc gctacggtgt atcagtcagc agtagtccag gaatggcccg aatacggcgg
      361 cacggcgtat cacttccgtg tcacgctcat cacggcgccg ctgtcgggta ctgcactgat
      421 tgataaaatg gtccgcctta tcaacgcata taaaaatacg cgctcctggc tggactacgt
      481 ccaatttatc cgtcggtgca gaggcgatgt taaatttggc gcgaacatga gcatcgtccg
      541 cagtacgacg attacatttg acctcaaacg gacgtacatc gcacaaaaga gcatccacat
      601 tgtcgccggc gtaggcgaat tcagggaggt acagattaat ggcgaattgg aataaaatca
      661 cgatgaccga cgtcggcgcg gcgttgcagg cgaaaatcaa cgccggcctg acgacgctca
      721 aatttacgcg tgtagctatt gtctccgttt cacgtactgg cgaattaggc agcgcgacag
      781 ggctagtcaa agaagaaatg acgctgggta ttaataaaat cacgcagagc ggcaacacgg
      841 taacaatcga attgactatc agcaacagcg gcgtcaagac aggttttaaa atcacggaaa
      901 tggggctttt cgccacggat ccggacgccg gagaaatcat gtatgtcgca ctgaccgacg
      961 ataacccgga ttatatgccg gctgagggcg gcagtactgt cgtacagcag gaattccagc
     1021 tgcaatttac gatgagcaac accggcagcg tatcggcgac gattaaccct aacggattcc
     1081 tgaccgtagc tcataacagc gatgaggccg cccacgaaaa catcctgatg gtgacgacaa
     1141 cggctaataa accgacatcg atgtcggagc gcggcatgtg ggttgagctc gtcgaatagg
     1201 gggcggtaat gtgctgaaaa tcaaaggcat ggacatctac tacatccgcg gcgacgatga
     1261 ctctttcagc gtgcagccga caacagcgga tggtacggcc atcacaggct atagcggcgt
     1321 cttctcggtc aagcggtcct acgatgacac ggcctacgtc ttgcaggtcc ctatggacgg
     1381 tgctgtcgtc gatttgacgc acgataagac gcagggcctt gcttacggtg actatgtctg
     1441 ggacgtccag ctggtgctgg aagacggtac gcatcagacc atcggcccgg gcaaattcca
     1501 cctgctgccg gacgtcacga cgacgtagga ggtgccacat ggataaaata agggctgtat
     1561 tgacggcacg gtcccctgcg ctgtctgccc gtctgagcgc cgggaatacc ctgacggtag
     1621 gcatgggcgt agccgggacc aaaggggctg tctatacgcc acacatcacg gatgacggca
     1681 tattatcttg gaccaatgac ggaggacggg acaatcccga cccggtggat atcaagggtc
     1741 caaaaggtga tacaggtccg caaggtccga tggggccaca ggggccggca ggaaaggatg
     1801 cactggccga caccatactg aacatggata ttgatgcgat tttttaacgg gaggaaacta
     1861 tggcaacaaa atttctggac ctcgatggcc tgaaatattt taaaacgaag caggatgcgg
     1921 cgaacgacgg gaaatatgtg ccccagggca tcaccatcaa tggcgtcgca ctcaataaga
     1981 ccggcatcac gattaccgat gacaccaaat taagtaagac cgatgcggcc agcgtttact
     2041 tgagtaagac ggatgcagct actacttatc tgggcatcag tgcgaaagcc tcgactgccg
     2101 gtgccgctga tacagcaacc aagttagcta cagcacggac catcaacggc gtgtcctttg
     2161 acgggtcggc gaacatcact attaatgcgg tcgatagcac agcacgtatc gcaacgtcgc
     2221 tcatcggcgc ggccaatggg gttgccccgc tcggatctga caaaaagatc ccggcccagt
     2281 acatcccagg cgatatcggc gaagtaatcg aaggctacta cagcggcggc aaattctaca
     2341 aagaatccac gcatacgacg gaaatcacag gctcgaccaa caccatgtac gtcgacattg
     2401 gcagcaccga caacgacata tacagatatt ccggcacggc ctacgtgctc atcaacgacg
     2461 ccgtcagcac tgccgacaag gccgtccggg atggcgacgg caacaccatt acgacgacct
     2521 atgtcaaagt cgtaagcggc aaagggctca gtaccaatga ctatacaacg gccgaaaaga
     2581 ataaactggc cggactcagt aactacacat tgccgacagc tacttccagc gttttagggg
     2641 gtgtgaaaat cgggagcaat ctcagtgttt catccggcgt catctcgctg acgtctgcta
     2701 atgtcacagc agccctcgga tacacgccgg caaattcggc cagcatagtc acgtactcgg
     2761 cgcttagcca gaccgagatc gac
//
LOCUS       DAWCYS010000227         2764 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1857_length_2764_cov_8.298265, whole genome shotgun sequence.
ACCESSION   DAWCYS010000227 DAWCYS010000000
VERSION     DAWCYS010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_2764_cov_8.298265"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          599 a          706 c          740 g          719 t
ORIGIN      
        1 acggcgtatg acggccgcct tcatctttgg tcaggacata gacctgagcc ttgaatttcg
       61 tgtgcggatg aatcgtgccc ggtttagcca ggacctggcc gcgttcgacg tctttgcggt
      121 cgatgccacg gagcagtgcg ccgatgttgt cgcctgcttc tgcgaggtcc aggatcttac
      181 ggaacatttc aacgcccgta acgacggtat ctttcggttt atcagcgagg ccgacgattt
      241 cgacagcgtc accgactttg atcgtaccac gttcgacacg gcccgtagca accgtaccac
      301 ggccggtgat ggtgaatacg tcttcgacag gcatcaggaa cggctggtcc gtcggacgtt
      361 tcggcgtcgg gatgtagctg tcgacagcat ccatcaagtc gaggatggat ttttcagctt
      421 ccggatcgcc ttcgagggct ttcaatgcag aacctacgat gataggtact tcatcgccag
      481 gataatcata ttcagtcagg aggtcgcgga cttccatttc aacgagttca atcagttccg
      541 gatcgtcgac ctggtcagct ttgttgaggt atacgacgat agccggtaca ccgacctggc
      601 gggccaggag gatgtgttca cgagtctgcg gcatcgggcc gtcagctgcg gatacaacga
      661 ggatagcacc atccatctga gcagcaccag tgatcatgtt cttgacatag tcagcatgcc
      721 ccgggcagtc aacatgtgca tagtggcgtt tttccgtttc atattcgacg tgcgccgtgt
      781 tgatcgtaat accacgttca cgttcttccg gagccttgtc aatatcggag taatcttcga
      841 acttggcaaa gcccttcttt gctaatactt tcgtaatagc agccgttaat gtcgttttgc
      901 catggtcgac gtgaccaatc gtaccgatat taacatgcgg ttttgttctt tcataatgtt
      961 cttttgccat tgtaatcatt tcctccttta ggaaaaaaac agctacccac ttgtattcta
     1021 tcacacatcc atactctttg cttaaaaaaa taggaaaatt taaacaaaaa atatttttgc
     1081 atatagaaat aacctcgtat gcacgaggtt attagtgtgg tggcggctcg gagattcgaa
     1141 ctctggacac tgcgggtatg aaccgcatgc tctagccaac tgagctaagc cgccatgtgt
     1201 ggagctattg accgggattg aaccggtgac cttatcctta ccaaggatac gctctaccga
     1261 ctgagctaca acagcttatg aactggtcaa agccaattat gaagttgttt ttttacctga
     1321 cagtaaaaaa atttggtagc gggggcagga cttgaaccta cgaccttcgg gttatgagcc
     1381 cgacgagcta ccaactgctc caccccgcga tgattggtgg tgggggaagg attcgaacct
     1441 tcgaaggcaa acgccggcag atttacagtc tgctcccttt agccactcgg gaaccccacc
     1501 atttactgga gccaacgata ggacttgaac ctacaaccta ctgattacaa gtcagttgct
     1561 ctaccaattg agctacgttg gcttactgtt ccgtggaacg tagtctatta tatcaaagga
     1621 ctgacgttct gtcaagaact tttttatcag cttttcttat ttcttctgac gaggcagaac
     1681 cccgtttcaa aaggctctgt cagaaccgct gacttgatta gtatactata ccccacggaa
     1741 taatgcaagt gctttttaca ctttttttaa attaatttta tctacccttc atgctgcgtg
     1801 tccaggttga ggaacagcgt gaactcgttc ttgaagtaga gcttgaccga cccgacaggg
     1861 ccgttgcggt gtttggccag gatgacttct gtaatgtgct ggttctccgt ttccttatcg
     1921 tagtaatctt cgcggtacaa gaaggaaacg atatccgcat cctgttccag ggcccccgat
     1981 tcgcggaggt cactgagcat aggccgttta tcctgccggc tttcgacact gcggctgagc
     2041 tgggacaagg cgatgagcgg tactttcagt tcccgcgcca gggccttgag ggaccgggaa
     2101 atctcggaga tttcctgctg gcggctttcg gcattgcgcc cctgcatgag ctgcaggtag
     2161 tcgacgataa tcaggtccag gccgtgttcc gacttgaggc gccgcgcttt cgatcgcatt
     2221 tcggcgacag agatgcccgg cgtatcgtcg atgtaaatgg gcgactcata aagcttgtcg
     2281 caggcatcgg tcaggcgcgt ccactcttca tcgctgttga gctggcctgt gcgcagtttc
     2341 tgcgagtcga tatgggcgat ttggcagagc agtcgctgga ctagctgttc ctgggacatt
     2401 tccagggaaa agaaggctac cgtcttatgc tgacggacgc cgacgttctg ggcgatgtgc
     2461 agggtaaagg ccgtcttgcc catactcgga cgggcagcga ccaggatcag gtccgacggc
     2521 tgcaggcctg acgtcatgcg gtccaggtcg cggaatcccg tcggcaggcc ggtgaggccc
     2581 gctttatttt catagagctt ggtgatcttg tccagggtca cttcgacgac ggcgccgacg
     2641 gaagcgaaat cgccgcggtt ctttgtatcc gacagctgga gaatgcgccg ttcggcatcg
     2701 tccaggaggg cgtgcgtttc cttttgtcgg tcctaggctt ccgtggtcag gtcgggacac
     2761 ggcg
//
LOCUS       DAWCYS010000228         2761 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1859_length_2761_cov_4.128973, whole genome shotgun sequence.
ACCESSION   DAWCYS010000228 DAWCYS010000000
VERSION     DAWCYS010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1859_length_2761_cov_4.128973"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    577..586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          626 a          743 c          717 g          665 t
ORIGIN      
        1 gccattgatg cccctaccta agaggaagga gtcttctggc gaaactgtga taaaatagag
       61 cggagctatc acaggaagca aagtctgctt tctgtaacag ctccgttact tttaaatcct
      121 tacttcttca aggaatcctg gcaagtctat tgtccatata agatttggca cgcatgcgtg
      181 ccaaatctcg cgtttccaaa ataggtagcc agttatggac aaattttctg tcttcactaa
      241 cagcagacag attcttgaat tggtcagata gtgtttttgt tctgccaaat agataaggct
      301 tcctagcatc tccagaagcc ccatcagacc gccgttggcg gccagtttcc ctaataatag
      361 cgacgtctga cataaaccgt caaaccatct tcggaaaatg attttttgac ggccaatatt
      421 ttagagatta tgaagcagca tggttaagcc atttttatgt atttgattat aaagtccagt
      481 acctgaatca ggactggact tctttggaca aggctcctgc cgccttggtt tcatcctgta
      541 tctaatacac atctgacgca gcccaccaat agagagnnnn nnnnnnccat ttttatgtat
      601 ttgattatta agtgcactag ctgaatcagg tctggacttt tttcgacatg gctcctgctg
      661 ccttggtttc atcatccaag ggcagccggg ccaggcgttc gataaaaaag atgcggcgat
      721 tgaagaaatt gacgaagcgc ccgacaagca tggccgatac cagggtgccg acgccgaggc
      781 cggtcagggt tcccaggcca ccataaagag tcgataaaat gacggctatg atgaccatgg
      841 tcgaatcaaa aacgcctttt gtcgacccca tccgccattt tcccgtctgg gcaatggcct
      901 tgacggcccc gtcgccaggc accatcagga cctgcgacgc cacttcgatg cagatgccga
      961 aggccagggt ggcacagccg gccactaaga gaactgcggc aaaaggccag ttcagtccct
     1021 gtaatcctgc catggcgtgc atgaaaaagt cgatggcggc actaaagacg aagttaatgg
     1081 caatttgcag cagctctacg ggccggaact ggcggcgcag cagggcaatc tgcaagacga
     1141 tgaaggccat gttgaggaca aaggtcagct ggccgaaggt ccacggcgta tagaggctga
     1201 cgacgagggg cacgctggaa atgggcgacg tccccaggct ggcgacagta atcaagtcga
     1261 tgccgaaagc cgtcatcaag atactgcaaa gaaagacact gacccgtatt ttcaaatgat
     1321 gcatatgacg catactcaat cgccccactt cttttcattc tccataattc gatgaaacgt
     1381 cgccacgaac tgctgtcgtg cttccggctc gataccctgc cacatttcat cgtcgatgcc
     1441 ataaatgacc tggcggatcc gcttcgcgtg ttcccagccc ggtgccgtca ggaagatggc
     1501 cacgccccgc tggtcttcga gccgcgtttc cttgcggatg aggtccagct tttccatgcg
     1561 tttcagcagg ctggtcaccg tcgacttgtc gatgatgcac tcgcggctga tatccttttg
     1621 gctggcaccg tcatggaacc acagatattc cagtatctta ggctgaccgg gcatgagccc
     1681 ttcccgcttg atgcggcggc cgatttcccg gttcgaccgg tgaaacccta acaacagtaa
     1741 aatatgcaac gcttcttcat ccatggcgat tcccctttta attagtttta attccaacta
     1801 cgttccttat tatatcgcaa gaaggagaaa agtcaacagg gatgcctctt atgaagcatg
     1861 catcatgatc cagtcgaagc agcgcgccgg caggatgctg tggaggaaaa tcaggggttt
     1921 ggcgccgaag ccgatgacgt agcgtgtctt tggccgtttc tggttaacgg ctttatcgat
     1981 ggccttggct acaacggatg ggtccgacat catagttcca gcatactgct tgcgtatgcc
     2041 tgcagccgtt ttgacagccg ccgcttcgta ggctccgcct tgggccgatt gttccaggtg
     2101 gtccgctgcg atatcgcccc attcggtctt gatgccgccc ggttcgatga gcgtgacgct
     2161 tcgcttcgtc cagggagacg gcgtcgacgg cgccatagga accgtagccg gcattattga
     2221 tgaggacgtc gatgcgccgc tgctcttcca gaatctggat gacgacctgg cggcacgatg
     2281 aggcgtcggt cacatcgagg accacaggga tgacgccgta cttctctttc aattctgcca
     2341 tgcgttccac gcggcgggcc gcaccgtaga tgatgtggcc ttcgttagcc aggcgttttg
     2401 ccgtttcaaa gccgatgccg ctgctggcgc cggtcaataa gataactttt ttattcatgg
     2461 tcatgtttgt ctcctttttt aaagtcacgg acggtctggc ccgtccaaat ggcaaaggcc
     2521 cgcaggaagg aattcacttc ggcgtagcct aacaaaaaag caatgtcctg ggtcgtcata
     2581 tccgtatggc gcaggtagtg gcgggccagg acttcccgcg tcccgctcaa tactttctgg
     2641 aacgtcgtcc cttcgtcttt caggcggcgc tgcaaggtcc gcgtactgat gcccattcgg
     2701 tcggccacgt gctggctcgt cacttccccg gctggcagca gctccgtcag gccactgcgg
     2761 a
//
LOCUS       DAWCYS010000229         2756 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1863_length_2756_cov_4.398741, whole genome shotgun sequence.
ACCESSION   DAWCYS010000229 DAWCYS010000000
VERSION     DAWCYS010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1863_length_2756_cov_4.398741"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    922..931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          503 a          787 c          875 g          581 t
ORIGIN      
        1 tatttctatt tcattatatt acaagcctga caggagtaca agcgcctagt cggcaccggc
       61 cagggccagc agggcttcaa tgggtgtcat cttgcctgta tcggtcgtgc gggacacggc
      121 cgtccgcacc aggccggtcc gggcatcgta ccatatttcg tcatacgtga gggcaaagtc
      181 atattctttt cccttgaggc gccgcgcctg gaactggcgg agcaggacct cgctgcagcc
      241 gtgctgccgg gcctggcgca gggcttctcc cagggactgt tccctgcggg caaagacagg
      301 atgcaggcgg tagccgagct ggtcgtcggc agcccgttcc agatagggcg tatcggcctg
      361 cagggcctcg tcgagggtca gggacaaggg cgggacccag accggtgagc cgaaaggccg
      421 gccgccatcg tctggcacag catggcccag ataggtttca taagccgtct tagcatcgta
      481 ataacggcgc agagcggtgt cggcatatcc ggctatatca tgggcaaagc ggcgcgtcgt
      541 cggcgctgcc ggcccgaccg tcttgtccgg gaactgttca tacgtgcggg aaacggcctg
      601 tacggccttt tctacgggat cgccctccat gacgatgcgg tccagccgga tgcgccggcc
      661 gtaagcctca ttaaaggcgt cattgccgac agccggagcg ccgaaggtca cgacctggag
      721 ctgcgacgca ccctggtcgg ccaggcgggc cgccgccagg acggcgacgg ctccgcccag
      781 gctgtgaccg gtcaaggtca gccgtttcgg tgcatgggca tcggcagcca gcacctggcc
      841 tacggtctgg ccgccatagg gcgctgccag aagggtcgtc gtgtagtgct ccaagccggc
      901 atggacgcgg cggccgccgt cnnnnnnnnn nctttccggg ccaggtggaa ctgggctgcc
      961 gatttatcat ccttctggac ctggctgtcg atgtgccagc cccggcgcac cagctcctga
     1021 cgggctgcca gaccgggcct gtcgccgtaa gaagccatgg ccatccaggc acagagggct
     1081 tcctgccggg cctgttcata agcggcgcgc ggcgaatcgg caaagacaga aaaagctgtt
     1141 gtcacgcaaa aaaggcacgt gaaaacagct ttgtaacaag aagtgaacat aggctttccc
     1201 ctttccagcc catcagggct cgtaggacaa aatggaaaag acttcccggt tcggatggtg
     1261 tgattcttca aaatagagag tccgcttcag ctcccgttcc cattcggctt tgcgcttctt
     1321 gtccttgagc agggccagga cgtcgggatg ggcttcgatg aggatatccc ctttgatgtg
     1381 gcggacctgg accatatggc gcaggcggcg gatgatctgg atggccacgg tttccggcga
     1441 atagaggtgg cctgaaccgc cgcagacgtc ccacgggctg aactggacct ggtacaggct
     1501 ctggcgggcc ttcttgcggg tgatttccac cagtgtcagg gccggcatgc gcaagacgcg
     1561 ggtcttggtg cagtcctgcg ccgtttcccg ggtcaggatc tggaggattt cgtcgcgttt
     1621 cgtcggctgg gccatgtcga tgaaatcgat gatgatgatg ccgccaatat cgcgcaggcg
     1681 cagctggcgg gcgatttcca cggccgcttc cttgttgacg tggaaaatcg tgtcctgcag
     1741 ggtcgaaccg ttaccggtgt acttgctgct gttgacgtcg atgaccgtca gggcttccgt
     1801 gtggtcgatg accagattgc cccctgacgg caggggcaag atgcgcgaca tgaggtgggt
     1861 caggctctct tcgatggcgt actgttcaaa gatggacacc gtcccttcgt agaggcggac
     1921 gcggctgcgc caggccgggt cgggaaagac ctggcagatg cgttcataag cttcgcggct
     1981 gtcgacgacg acttgtttga cgtcggccgt gaaatggtcg cggatcatgc gcatgaccag
     2041 gtcggcttcg cggtataaga gggtcggctt gcgggaccgc ttataccgct ggaggatgct
     2101 gtcccacgtc cccaggagat actgcagatc cttgaggagc tcttcccggg aaacgccctt
     2161 ggcgacggtc cggatgatga ggcccatgcc gtcgggcttg atttccgcta cgatgcggcg
     2221 caggcgcttg cgttcttctt cgtcgcggat gttcttggac acgccgatgt aatcgacagt
     2281 cggcatgagg acggcgtagc ggccggccag gctgatattg gccgtgactt tcggcccctt
     2341 catgccctgt tcgtccttga tgacctggac caggacggac tggcccacgg agaggtgcat
     2401 ctgctggatt tcttccttcg acgccgcccg cgggaacatg tcgcccatat agatgaaggc
     2461 gttcctgcgg cggccgatgt tgacaaaggc agcctgcagg gacggcagga tgttctgaat
     2521 cgtcccttta tagatatggt tgacaatgtg catgtcgtca ttgcgttcga cagcgaaatc
     2581 acgaagacga ccatcctcca ggatagccat gcgcgtttct tctggcatga cattgcctac
     2641 gattgttttc atcttctcat tcctcctgtg cgggcaaatt ggtaatcgca gacaggctat
     2701 ttcactaaca agacggcgaa ataacccgtt tctttggcgt cagccgcttt ttcata
//
LOCUS       DAWCYS010000230         2732 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1875_length_2732_cov_3.881957, whole genome shotgun sequence.
ACCESSION   DAWCYS010000230 DAWCYS010000000
VERSION     DAWCYS010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_2732_cov_3.881957"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          608 a          734 c          689 g          701 t
ORIGIN      
        1 ggccggtgtc gcccgcatca acctcatcga ccccatggaa tatgaaccct ttgccaatct
       61 catgagcctg agcagcctgg tcctcaccga ctctggcggc atccaggaag aagcgccgtc
      121 tctcggcaag cctgtcctgg tcctgcgcaa tacgaccgaa cggcctgaag ccgtcgaagc
      181 cggcacggta cgcctcatcg gcacggacaa ggatgtcgtc tatgccgaaa cgaagcggct
      241 cctgaccgac caggccgctt acgatgccat gagcaacgct gtcaatccct atggcgacgg
      301 caaggcttcg cagcgcatcg tccaggctat tctccacgtt ttttccgggg aagaagccgt
      361 tccggatgat tttagccgct aagtattgtc ttaggctata ctgagaatcg tttttagcta
      421 taacgtttaa atttctttca ttttgtattg attttccaga ttttccgtgc taaaataagc
      481 caataatcgt tgctggcagt atataactgc tataccaaac gattatggaa aactgccatc
      541 gcccgagcag gagtgtatgt agtatgagac atcatgatga tcctcacgat gacaaattaa
      601 atcacatgag gctttgggat attgttgtca ttgccggttc cttaggtttg tctctcttcg
      661 tctgcatctg tgtcggcgtc gccatagggc acttcatcga tgtccatgcc gggacgtcgc
      721 cgtggggatt gattcttttt tccttcatcg gtgccatctc cggattctgg accttatata
      781 aaaaagcctt gtcctatcag caggacgagg cgcgccatgg ccggcatctg cgcaagtgaa
      841 atgtcacgga ggatagccta tggaagcgtt ttatgcttat gtaaaggcaa caatatacaa
      901 gctcgttggt tttacgtgta tcgtcacggc cgtccttttt ctaggaggct ggtggcagta
      961 cataagtggc tggtgtatcg gcagcggctt gaacatcgtt tatttcttca tgctcagcag
     1021 ccgttcggta cgggccttaa aattgccgcc tgggcaggcg gcgtcattca tccgcggcgg
     1081 tgccgtgttc cggctggtct tcatctgcct ggccgtcatc gtcatcctgc agttcccgtc
     1141 catttccctg ggggctgccg tggccggtat cttttcgtac cgcgtcctcg tcttcgcaga
     1201 tgtactgact gcgcgtctca gacggtaaag gaaaggaggt attgcgtatg gaattacatg
     1261 cagggcatca tatggtcgtc cagctcctgg gtctgaatgt caacttggat acgctcatga
     1321 tgacctggct ggttgcggct ttggtcatta tcgtcactgt cgctgcgaca aggggccggt
     1381 ccctcgtccc gtcaggactt cagaacgcca tggaaatcgt catcgaagca ttgctcgatc
     1441 agttcaagga aacgttgggg ccgaaatacg gccaggtcgt ttcggtcttg ctgacgatgt
     1501 tcctgttcat cctcttcgcc aacgaattgg gcatgctgcc gaatccgcat gtcctggctt
     1561 cgccgacgaa tgatttgaat acgactgtcg ccctggcact ggtttcttcc tttatggtcc
     1621 acttcatggc cttgaagaac caggggttca aaaagcattt caagcatttc ttcgaaccct
     1681 ttgcgccgtt tgtcgtcatc aatctgatgg aagaaatcac caaaccgttg acactggcct
     1741 tccgtctctt tggcaacatc ctggcctgtg aaatcctgtt ggaagtcctg tacttcctcg
     1801 tgcctgtcgc catcccgatg atttggatcc tctttagctt cgtcatcggc ctcatccagg
     1861 cattcatctt caccattctg actacatcgt atctggcacc gtcctttacg gaagactaag
     1921 aaaagctcag ataccgttac aacttaggag gttatttatt atggaaaaag ctctcgtatt
     1981 ggcattatct gcatttggtg catgcttagg tattggtttg gcagctctcg gcgctgctat
     2041 cggtgacggc catgccgcat cgaagatgct tgaaggtacg gcccgccagc cggaaatggg
     2101 cggcaaactc ctggtcaaca tgcttatctc catcggcctg atcgaatcca tgccgattat
     2161 cgcagctgtc atcgctatcg tattggtctt cgccaatccg ttcgtcagcc tgctctaatc
     2221 cgaagattct aatatacagc cggggaggga agtacattgg ttagtattaa tggtaccttg
     2281 ctgttccagt tcatcaactt cttcgtgtta gttgccattc tggcaaagtt cgcttataaa
     2341 ccgctgctca aagtcctgga agaccgccgc aacaagattg cgtcggacct cgacaacgca
     2401 gccaaagccc gggaaacggc tgaaaagatg aaagccgact atgaagccca gatccgcgat
     2461 gcccgtgctg aagcccaggc cattgtcgac aaagctgtca agcaggccga caaagaagcc
     2521 caggcccagc tggaagccat ccgcgcccag atcgcccggg aaaaacaaat cgcccagacg
     2581 gaaatcgcca acgaacggga agcggctatc cgggaaatgc gtaatgaagt agtcacactg
     2641 tccatggctg tggcagaaaa actgctccag aaaaacatgg atcctgacat gaacgccaaa
     2701 ctggtagccg attgcatcga ccagctccag ac
//
LOCUS       DAWCYS010000231         2727 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1879_length_2727_cov_7.976422, whole genome shotgun sequence.
ACCESSION   DAWCYS010000231 DAWCYS010000000
VERSION     DAWCYS010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_2727_cov_7.976422"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          868 a          638 c          600 g          621 t
ORIGIN      
        1 gtatagaggg catccgtatc ccggccaata tcttcataaa tgaagccttc ccgtatcaac
       61 gccgtcacat ggcgcccctg taaaagagac agtaaatccg tctccgtttc agccgtcaca
      121 tggcgcagca tttcccggat aatgccattt cccgacgtat tgacccaata atctccgaca
      181 tcaccgtgac ggaaaaaatt gacaatcgac cagggattat aaatctccgc cccaccgaag
      241 agatagccat cataccacgc tttcaaggcc ggaagggccg tggtcatgga aaggtcttct
      301 gccatctggg ccacttcctg tggcgtaaat ccaatgacat cccgataggc agcatccatg
      361 acggagcaaa cgtcgagatt attcaaatcg ctgaaaatac tctctttggc cacccgcagg
      421 acccccgtca gtacggcaaa atccaagtcg cgattgccct tcaggacact gcccaggaac
      481 tgtttcataa aagcgatgca ctctgtataa aagccatgtt cccaagcctg ctgaatcggc
      541 gcatcgtatt catcgagcaa taaaatgaca ggcttgccga aatgctgatg catcatcttg
      601 accaatagtg tcagggccag ctgcatttca ttctgcccgg ccccctgctg tttcagtgcc
      661 aggaagcgcc tgcgtaaatc cgggttcaca gccggactct ctgccaaata ctcatggtcg
      721 atgaaccaat aactaatcca ggaactcagg gattgcagca tctgcggcca agtaaggtcc
      781 ttcaaatctt tcaaagacaa gaacagcacc ggatactggc cgcgatgggc catgtaggca
      841 gcgccggctc cttcgatagc cagtccgtcg aagagacggc ggctctgtgc ctctttttcg
      901 acagaaaaga aatgctccag catactcagg gtcaaggtct taccgaaccg ccgcggccgc
      961 gtaatcagcg tcaccgcact gtgcccgtca atgagctggc ggataaaatc cgtcttgtcg
     1021 acgaaataat aatgcttact ccgtatatca aaaaaattat cgacaccaat cggcatctgc
     1081 cacttcatac catcaccttc tttcataggc gggggccttc tcctgttctt tgtcttttat
     1141 tataacaaga ctgacgagaa acaaaaagag gagacacgca atgattggtg agctgcaccc
     1201 caatagttag accaaaaatc taacgattgg gggtcagttt ttttatggca aaacacagtt
     1261 atgaatttaa gaaaaaaata gtactggaat acttgagcag tgacgaaggg tgtacttcta
     1321 tttcacgtaa atatggaatg gaaagtagca gccaattgct aaagtggact gcagcctaca
     1381 aagcatttgg agatgatgga ttgaaaagat ctcgctctcg aaaaatatac tctttcaaag
     1441 agaaactttc tgtggtacag tcttacttaa caagcgaatt ttcatatcag gaactagccc
     1501 ttcaagtagg aatcaacaat ccgacaatga ttgccagatg ggtcaatgaa tttaaaatag
     1561 ctgggccgga tgcattacgg ccacatagga aaggcaggag aagaactttg agcgaatcac
     1621 gaaccagaaa accagatact caggagcaga agccagtcgt cgatgccagc gtggaacatg
     1681 tccaggagtt agaggatgaa aatctcaaac tccggataga gaatgctttt ttaaaagaac
     1741 tgaggagact gcgtttagag gacgaagcaa aaacgagagg gttgcaaaaa tcatctgcag
     1801 tctccgaaga tcattcaaat taaacgatct tctctcctat acgcagatgc ccaaggcaac
     1861 ctatatgtac tggcagaaac ggtttgaccg tgagaatccc gatcaagaac tagaagacaa
     1921 aatacaggaa atccgtagcc agcataaaga ttatggctat cggcgcatga ccggtgaatt
     1981 aaaaaatcaa gggtattgcg tgaacaagaa gaaagtccaa cgtatcatgc agaaactagg
     2041 ccttcaggta acatccttta ctcgaaaaag ccgtaaatac agctcttata gaggcaaggt
     2101 aggaaccgtg gctccgaatc ggatacgacg tcgttttaac acgactattc cgcaccagaa
     2161 gattacaacc gacacgtcag aattcaaata ctatgaagta gatacacagg gacatctgac
     2221 cctacacaag ctttatctgg atcctttcct ggacatgttc aacaatgaaa tcatcagtta
     2281 tggaatagcg aaacatcctt cggctaaaag catactggaa gctcaggctg aagcaatcaa
     2341 aatcacggct gattgtccgt atcgaagaac gtttcactcc gatcagggct gggcatatca
     2401 aatgaaagcc tatacccaga atcttaaaga agaacgaatt tttcagagca tgtctcgaaa
     2461 gggcaactgt catgataacg ctgtcatgga gaacttcttc ggcttaatga agcaagaaat
     2521 gtattatggg gtgatttact acagctatga ggaactaaaa gatgctattg aacgatatat
     2581 caaatactat aatgagcaaa gaatcaaaga gaaactaggt tggaaaagtc ctgtccaata
     2641 cagaattttc cgacagacag cataaaaaag taacgagaca gtaaaaactg tctcgttacc
     2701 aaggtctaac tttttggggt cacatca
//
LOCUS       DAWCYS010000232         2726 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1881_length_2726_cov_4.378884, whole genome shotgun sequence.
ACCESSION   DAWCYS010000232 DAWCYS010000000
VERSION     DAWCYS010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1881_length_2726_cov_4.378884"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          457 a          795 c          868 g          606 t
ORIGIN      
        1 acgacgttgc tgtagacgtc ctggctgccc aggggaacgc ccaggcgctt ttccaggacg
       61 gccaggagga tgatcaggcg gttatagtcc atgcccgatg ccgtccgccg cgggacggcg
      121 aagacggaat gggccgtcag ggcctggatt tcaccaagca tgggccgcat gccatccagg
      181 acggcagcta cggctgagcc ggcctggtcg gccgcccgtt cggccaggag cagctgagac
      241 gggttctcca gttccgacag gccgctgctg gtcatggtga acaggcccga ttcattggtc
      301 gagccgaagc ggttcttgat gccccgcagg atgcggaact ggtagttccg ttcaccttca
      361 aagtagagga ccacgtctac catgtgttcc atcatgcgcg gcccggcaat ggcgccgtcc
      421 ttggtgacgt ggccgatgac gacgatgggg atgccttcgc ttttggcaaa ggtcatgagg
      481 cggctcgtgt tctcgcgtat ctgggccaga ctgcccggcg tcccttcgac ggactggctg
      541 tacatggtct ggatggaatc gacgaccagc agggccggcc gccgcttctt ggcttcgtcg
      601 aggatatggt ccagggatgt atcggcctgg atgatcaagt ccggcgtgga aatgcccagg
      661 cgctgggcgc gcatcttgat ctggcccgcc gattcttcgc cagagccgta gaggacggtg
      721 ccgttcttgt cacagacggc agcggccagc tgcaggacca gcgtcgactt gccgatgccc
      781 ggatcgccgc tgagcaggat cagcgcccct gggacgatgc cgccgccaag gacgcggtcc
      841 agttcgccaa tgcccgtttc catgcgggcc atgtcttcca tggcgatatc cgccagggac
      901 gccgcctgcg acgccggacc gctgcgagcc atgctctgtg ccgctttggg cgcttccggc
      961 gcgaccgtct cttcaaccat ggtattccat tcgccgcact gcgggcagcg accgagccag
     1021 cggctggaca cgctgccgca gttactgcag acgtaacggg tctttatctt tgccatgact
     1081 gctccttact ccgtcgtaaa gacgagtttc ttcttgtcca ggctgtcgac atggatcgta
     1141 ttgccttcct tgacactgct gctgaggagc atttcggcaa tcgggtcttc gacgagcttc
     1201 tggatagccc gcttcaacgg ccgggcgcca taggcgaagt ccgtcccttc ctcgactagg
     1261 tcgtcgtcgg cagccgttgt ccagtcgagg gcgacaccct tttcaccgag gcgcttggtc
     1321 aggtccttga gcaggatgtc gacgatctgg cgcaggtctt tctgttccag cggcttgaag
     1381 acgatgattt cgtcgatacg gttgaggaat tccggccgga agaagcgttt cacatcggcc
     1441 atgatttcct tcttggctac tgcaaaagat gcttcccggt ccttggcgct gtcgccgccc
     1501 gtcgagaagc ccatgaccgg gcccgacggc ttgagatgcg atgcgccgag gttgctggtc
     1561 atgatgatga ccgtattgcg gaagtcgaca gtccgccctt tcgaatcggt caggcggcct
     1621 tcgtcgagga cctgcaggag caggttgaag aagtcgctgc tggccttttc cacttcatcg
     1681 aagaggatga cgctgtacgg cttttcccgg acggcatcgg tcagttcgcc gccttcttca
     1741 tagccgacgt agcccggagg cgcgccgacc aggcgggaaa cgctgtattt ttccatgaat
     1801 tcgctcatgt cgatgcgtat catgttgtca gcactgccga agagctgggc tgccagggtc
     1861 cgcgccagtt cggtcttgcc gacaccggac gggccgagga agaggaagga accgataggc
     1921 cggttggcat ccttgaggcc ggcgcgggcc cggcggacgg ctttggccac agccgtgacg
     1981 gcatcgtcct ggctgatgac ccgtttgtgc agttcggctt ccaaatggag gaggcgctgc
     2041 gattccgttt ccgtaatctg ttggaccggg acgcctgtcc acatggatac gacgtcggca
     2101 atgtcgtttt ccgtaacgac gagcttttca tcgtcgtgct gcttcttttc tttctgaagg
     2161 gccgagattt ccgacgatac tttcttgccg gcatcgcgga gggacgcgca tttttcaaag
     2221 tcttcgccag ccagggccgc ttctttttct ttattgatgt cggccagttt ctgttccagg
     2281 tcctgaagtt catgggacgg cgcatagacc ttcatgcgga cgcgggccgc cgcttcgtcc
     2341 atgacgtcga tggccttgtc cggcagataa cggtcggaaa tgtaccgtga cgacagtttg
     2401 accgcagcct ttacggcttc atcggtgatg tgggccttgt ggaaggcttc gtagcggtcg
     2461 cgcaggccga agaggatagc catggcatcg tcttccgtcg gttcgccgac caggacgggc
     2521 tggaaacggc gggccagggc ggcatctttt tcaaagtgtt tcttatattc gtcgatagtc
     2581 gtggcgccga tgcactgcaa atcgccgcgg gccagggccg gtttgaggat gttggccgca
     2641 tccatggcgc cttcgacagc accggctccg atgagctgat ggaggtcgtc gatgaagagg
     2701 atgatggcgt cgtcctcttc gagcgg
//
LOCUS       DAWCYS010000233         2711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1889_length_2711_cov_4.519202, whole genome shotgun sequence.
ACCESSION   DAWCYS010000233 DAWCYS010000000
VERSION     DAWCYS010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_2711_cov_4.519202"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          533 a          900 c          852 g          426 t
ORIGIN      
        1 cagcaacacc agggactgca cgacggctcc ggcagcgccg gcagcccgac agctagcggc
       61 gacacattcg gccggctagg cgccgaccgt ccagtcaccg ctcccgtcac cgcctttcga
      121 cggacaagcc gcctcgaagt cataaggccg cccttcttca ctgaccaggc cggcaccggc
      181 gatgagggca tcggcttcct gccggtctgt cgtccccagg gacaggcgct tccaaaggac
      241 gctcggcccg ccgatgagga cgcgcaggcg ccagccagcc aggccatagc gctgcaggag
      301 ttctggcaga caggactgga cgcccccttc ctggcccatc cagtccgggt cccgcttggt
      361 ttcatagtcg cgatgaccct ggcggaaggc cacgcgtcca taccaggtcc gtttcgtctt
      421 caaaagatgg acagactgtc cattcagcca taaacaggca ttttgttgcc accacataag
      481 ctcacctctc tactaattga catactgtac gaaatgcagc acggtccggc cctgttcatc
      541 ggccgtcacg gcaaagtgca ggccgcacgt ctgctggacg cccgttcccg gcgcagcggc
      601 ccgggcctgc agatagccgc ccggcgtctg accggccggc tgatactggc agcggacggt
      661 caggatgtcc ccagggccag ccagcccttc tgcccggggc acggccatgg cctggcccgc
      721 cagaaaagcg tccgggtcct gccgcagctg gttccagccc tcacagaggg cgctttcggc
      781 caaataggtc agccgcacgg tctcttcata ggtccgggcc atctggaagt gctgccacgc
      841 cagggtcgcc gccgtcaggg ccaggctcag ggtcagtacg gcgacggtca cgatagaaaa
      901 agaaaagaag ccattgcgtc gtttcatgat tcaccttcat tctggggaca cacggcaaac
      961 tgcacgggcc gggacaggcc gctccgccgt tcttttagga gaaaggcgac ttgtacggcc
     1021 tggccggtcc gggagaaaaa gggccaaccg tccaggggct ggatgaccag ggcccggccc
     1081 tggacggtgc tgccgccgcc tgtcaggggc tgatagttgc catcgctgag gcggacatac
     1141 aggccttgat ggcggacggc aaagcccttc acggcgccct tggccgtgcg gaagcggacc
     1201 tcctgaggac ggacttcaac gtcccggcca tagcgcaggg cctggaacag cgtttcttct
     1261 acgctgacgt gctgccgcag caggccttct tcaatctggc agcgccaatc caggcgggcc
     1321 gtctgtcccg ccagaggcag caaagccgtc agagtcaggg ccgacaggct caaggccagg
     1381 agtacgtcca agaggatcca gccgtttttc ttcggtccct gccggcacca gaagccggca
     1441 ggaaacatgg gggccatggt catcacctgc ctctacctgc caaaggacat agccctcgcc
     1501 gagagccgtc tgcgcatgcg tcacctcata ggtccggccc tggatgacga cgcggtctga
     1561 tggcggacag ccgctcttcc agccttccat gacctgccgg cagaccatgg ctgccagctg
     1621 ccgctgtctg gcctggtcat gcagtctgac ggcctggagg gaaaggccca gggccgccgt
     1681 gaacaagcag gctccgagga gcaggcccgc caggccgtcg gtcaggaaaa agaagccatt
     1741 ggcctcaggc gatacggatg cgcccggtct gcgccgccac gacgatagaa cgcgtatagg
     1801 ccccatcggg cgaagtaatg accacagcca tcgtatgctg cggacgacca tcgacagcaa
     1861 agtgtacatc tcctttcgtt acctgcaggc ccggcggata gggccgttct ttgaccggga
     1921 tgtagccttc ctggaggacg tagcgctttt cttccaggac cagtttccag cggccgttgc
     1981 tctggttgtt gccgacacag cgctggcgga cctgctgcat atccagggcc atttcccgcg
     2041 ccaggccatc gacggcctgg tggctgcggc tgcgctgacc ggcagtgagt cccaggccca
     2101 gatacaagag cagcaggccc agggccagca tcaggccggt caggctgata aagccgttcc
     2161 gcctcaccat agccagcctc cgggccaggc gcgctggagc ggttcctggg ccagccacca
     2221 gccggccagg aggaacgggc cgaagggaat gatcgtcttc cctgtgcgga aacggtagaa
     2281 caaggccacg agcgcggcca ggccaaaaga agtgactgcc atggtccagg ccccccagcc
     2341 cgggcagccc ggcgccaggg ccagggccag cttgatgtcg ccgctgccga tggaaccggg
     2401 ccagagacca tagaggagca ggaagagggc ggcgacgccc agggaaacgg gccaaaaggg
     2461 ccggaccaga ccggcataac tggcggcgcc gttgcccaca gccagggcca ggacgggcgc
     2521 gtcggcaatc cagcagcgcc ggatatcggt atagcaaatc caggccgtac agccggctgc
     2581 cgcagcgtac cagagagata caacagaccc tcccatccta tcaggcctcc ccttcctggg
     2641 gcgatgccgc atcggacgag aaatgcttgt tgttataatc ggcggaaacg acgccatcag
     2701 ctatctggta t
//
LOCUS       DAWCYS010000234         2710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1890_length_2710_cov_7.294539, whole genome shotgun sequence.
ACCESSION   DAWCYS010000234 DAWCYS010000000
VERSION     DAWCYS010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1890_length_2710_cov_7.294539"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          627 a          650 c          689 g          744 t
ORIGIN      
        1 atggcatcga gtaaataggc atagacgacg atccataaga agaccttgag cttcatgagc
       61 ttggtcagct tattcacagg cgccgcatcc ttcacaattt tcttccgggt ccccgaagag
      121 gcgggctttc tggccgcgca tagccaggac ggcctgaatg tacatggcac attcgatgcg
      181 cttcttctgg atcttgcagc tcagttcata cggcgtctgg atgttgccgc cgtacttgat
      241 gttattggca tggcagccgc cgctgcagaa gaacttggcc cagcagtcgc agcagatggg
      301 cttgttgagg acgtgcatgt cgcggaagag cggcggcagt tccgtattgg tcacgccgtc
      361 atagacgttg ccgatgacat agtcgtcctg gccgacgaac tgatgacagg gatagatgtc
      421 gccgttgggg acgacgcaca tgtattcatg gccggcacca cagccgcgca ggcgcttggc
      481 gatgcaggga ccgcgataga ggtccatgat gaagtggtaa taaatgaagg gccggccttc
      541 atccatgcgt ttcaggtaga aatcggccag gcggtcgtat tcttcataaa tgcggggcaa
      601 gtcatcatcg gtgatggcat aggacaagtc gtcgccgacg acgggttcca tggacaggcc
      661 ttcaaagccg aggtcagcca tgtgttctac gtctttggtg aaatcgagat tgtattttgt
      721 atacgtcccg cggacgtaat attcttcacc gttgcggtgt ttcgccgcat agacctgatt
      781 gtcggcacag gtcttgtacg agccgtgacc gccggcatcg ggacgcatgc ggtcgtgtac
      841 ttccggacga ccgtcgaggc tcaggatgag gctgatgtga ttatccgtca aatacttgac
      901 cttggccggg tcgaggagca tgccgttcgt cgtcagggac agcttgaata ctttatcgtg
      961 gatcttttcc tgttcccgga tgtactcgat ggtctgcttg acgacgccga agttcatcag
     1021 cggttcgccg ccgaagaagt cgatttcgca gtgctggcgt ttgccttcgg tgctcttgat
     1081 gagcaggtcg acagcgtgtt tggctacgtc gaagctcatg agttcccgct tgtgcgtatc
     1141 gtaatcgccc ggggaggcaa agcagtattg ccagcggagg ttggactcgt gggcaatgtt
     1201 caggcagagc gatttgatga ccggttcttc ttcggcgacg accttgaagt tttccgtcat
     1261 cggtgcaaag agcatttcct tttcgatgag ctcatccagt tcgcccaggg tttcgtccaa
     1321 atccttttta tcatacgtcc cggcaaaggc ctgatagaca gcgtctttat tggtaccgtc
     1381 atagacgtcg aggacatcgt aggtcatctt gtcgatgacg ttgatgatgc cgctgttgac
     1441 gtcgaggagc atgtacgtcc cgttctgaca gtatttatga atcattggtt taatttcagc
     1501 catgaataat gtttcctcca tacaaaaaga agggggtgcc gcgtgagcga cgcccctttt
     1561 ccttcacgta acttattttg tgcagatctg attgccaaca gtgcaagaag ttttgcaagc
     1621 ggactggcag gacgtctggc attcgctgca gccagcatgc tgtgccgtct tctggatcga
     1681 ttctgtgttg attgtaatga tgtgttttgc catgatattc acctcgctat caatgattat
     1741 tccttatttt accacattct cggtgattct acaatatgac aggattacaa gagtttgaag
     1801 ccttcaaaga agaaattctt gcccatgctg atgacgatgg accgttcatc gaatacgtca
     1861 ttggtatcga agaatacgtc gaagcctttc ttataagtag cataaatcga agtgatgcga
     1921 cttaaataac tgcggatttc ctcggctgtc acttccggtt cgccgtcaga gcgccatttc
     1981 ttgttcttga aattcgcaat cagcggcgca tgagcgatga cacgggagcg gtgcgccaaa
     2041 acccagttcc aaactttttc accgtactgc tgtgcttcct cttcatgacc ttcttcgagg
     2101 ccatcgaggg taaacaggac aggccggcct tcgtacatga tttcatccat gtaactgcct
     2161 ttgacatatt ttaaattcac agaagcaccc cctgcaaaat catgtgaaac atgacgaata
     2221 tttatgtcta acagtaatca ttgtatcgtg cttccgggat attgtcaata aaaaggtccc
     2281 gtcgcatagc gacaggacct ttttgagtgg ttagcagcta gtggctagtg gttagcagcc
     2341 ggttaaaaat ttgaaaccat ctgctacaaa ctacaaactc tatactacaa actaattaat
     2401 ccatttttac gaaagcgtct ttatcagcgc cgcaggtcgg acatacccaa tctgccggga
     2461 gatcagcgaa tttcgtgcct gctgctacgc cgttgtctgc gtcgccttca gcttcgtcat
     2521 aaatataacc gcaaatgctg cattcgtact tatccatgtg tttttcctcc tgaattaaca
     2581 tattaacgaa taattcttac taatgatttt gacacgctta taatatcata cttgcctaaa
     2641 aaaagcaaga ttattttatg actgattatg cgtgtctgcg catattgata tcgatgatgc
     2701 ccgacgaata
//
LOCUS       DAWCYS010000235         2698 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1898_length_2698_cov_5.136587, whole genome shotgun sequence.
ACCESSION   DAWCYS010000235 DAWCYS010000000
VERSION     DAWCYS010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_2698_cov_5.136587"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    416..425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    789..798
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          537 a          738 c          742 g          661 t
ORIGIN      
        1 ggcaaaatgg gcatctcatg ttttttcctc ctaccttctt ctcgacggta tttttaaaaa
       61 aaagcgggtc cgccgggatc cgcttttttc atgctgatta tctcatccac gtaggaattt
      121 ctacgccgcc ggcattgtct ttcgtatcgc cggctttggc tgctgtacca gtgccctggc
      181 caaatgcggg aatcgaagac ggttctttgc caaaacccgt agccacgaca gtgatgcgga
      241 tggaatcttc catatccgga tcaatgacgg aaccaaagat gatgttggct tccggatcga
      301 cagcttcagc cactttttca gctgcttcgt tgatttcgta caagctgata tccgggccgc
      361 cggtaacgtt gagcaagatg ctctgggcac cgtcgatgct cgtttccagg agggcnnnnn
      421 nnnnnttgca ccggagccgt cgccgatacc catgatagct tcgccctggt cggacatgat
      481 ggtcttgaca tcggcgaagt cgaggttgat gaggcctgtc gtcgtaatca aatcggaaat
      541 gccctgtacg ccctggcgga ggacatcgtc agcgacgagg aatgcgtcct tgaggggcgt
      601 cttcttgtcg atgatctgga gcaatttatc gttagggata gtgatgatcg tgtcgacctt
      661 ttctttcaag tagtcggcac ctttatcagc gacttcctta cggcgtttcc cttcaaaaga
      721 gaacggcttg gtaacgacag ccaccgtgag ggcccccatt tccttggcac attcggcaac
      781 gataggagnn nnnnnnnnga taggagcagc accggtaccg gtaccgccgc ccatgccggc
      841 tgtgacgaag accatgtcgg caccctgcag ggccttgacg atttcttctt tgctttccag
      901 ggcagcctgt tcgccgacct ggggattggc accggcgccg aggccgcgcg tcaatttttc
      961 accgatctgg atcttgacat cggctttgga tacttccagg acctggtctt ccgtattgac
     1021 cgaaatgaac tggacgccct gcaagccgct ttctatcata cggttgacag cgttgttacc
     1081 gccgccaccg acacctatta cttttatgtt tgcaaactct gccatatcgc attttcctcc
     1141 ttcagactct ataacgcaca ccctgtgcag taggattcct tattatttta cactatctta
     1201 gaaaatttat ctacctgttt ttgaaaatca aggccctatg cgccttattt cgtcttgaca
     1261 aggggcgagc gcacatccgt gtcgatgtat tggacggcca gatgattttc ctgaacttcg
     1321 gccagcagcg tttccgtcag cttcgcccgt tctgccggtt catcgccgct gcccaggtgg
     1381 atagggatgg aatcggtcgt ataggcgacg atgctttcgg gatttcccac gttgatttca
     1441 gcgatattct tgagaatatc cggcggcagg ctctggagat agaccatggc cgaatggagg
     1501 gtcggatctg tcaccgtatc gcctaagagc aggttatcga ctttcttgcc cgtcatgatt
     1561 ggcagggata cacccttgat ctgcggcccc agttcgatga cccgccccgt cttgtcgaca
     1621 taggcgaagc cgtatagcgt cgtgatgaca gcggctggct tgcgttctgt cagggtgacg
     1681 tggatcgtcg ccgggaattc ccgcgtcact gtcgcgtcgg caatgcgcag gtctttcttc
     1741 aaccgttctt ccacatcgtc tgtcgacaac tggacgacgt tgacatagga ataagccccg
     1801 caggcccggt agacgtcttc aaaggacatg ttctcgttgc catcgacgat gatcgtcccg
     1861 aaagagaccg gcgccaggcg caggaagagc cagataacga agacgccgag gagcgtacag
     1921 ctacagccgg tccggcggcg ccgggcccaa atctggttct gccgagcctt gttcttgttc
     1981 cgccgtgctg cctggcgggc cttgcggcgg cgctgggctt ctttcttggc ggccagggct
     2041 tcccggtccg gttcgttcag cgggtcgttc tcaagatgag gaggaacaaa cacatcatga
     2101 gaatcgttag gcagcacgac ccgtcgctgc cccgtatgat atgatttctt tcccattaaa
     2161 acttacctac cgatgcttcc agcaaaatct tttcacacaa atcttcaaag gacattccca
     2221 tggcccgggc ggcatcgggg accaggctgg tcgccgtcat gcccgggacg gtgttgattt
     2281 ccaggacgaa cggatggcct tctttgtcgg tcatgatatc gacgcgggat acgccgctgc
     2341 agtggcacag atggaagaca ttcagggcca ggtcctgcat ttctttggtc agttcctgcg
     2401 aaatcggagc cgggcagaga tgcttggcga cgccaggggc atacttggaa tagaagtcgt
     2461 acttgcccgt gctggacacg atctggatga ccgggaaggc gacgtcgtcc atgacggcga
     2521 cggtgaattc atcgccatcg atgaaggctt cggctaccag cgaagcgctg tacttgaagg
     2581 cttcttccag ggccggacgg agttcctcct tggtctggac aatgaccgtg ccgatactcg
     2641 agccctgacc ggacgacttg atgacgacag gcaggtcgaa gcgctgttca atgtcggc
//
LOCUS       DAWCYS010000236         2691 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1903_length_2691_cov_4.703718, whole genome shotgun sequence.
ACCESSION   DAWCYS010000236 DAWCYS010000000
VERSION     DAWCYS010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1903_length_2691_cov_4.703718"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          609 a          811 c          757 g          514 t
ORIGIN      
        1 tcgaaggaca gctctcaggc cgtcgtcacg ggccagacgt cggaagacgg caaggtcgtc
       61 aacttgtccg tcgaatatcc gtcgtcccag agcagccaga gctcgccgtc gagcagctcc
      121 agcggctccg atggcagtgc caataaccag gaaccggccg ggacgcctca ttacgggacg
      181 gtcaatatca gcgtaccgtc tggcgcttcc agccagcatg tccagatcgt cgtatccgac
      241 gacaatggct cgcgcgtcgt ctatgaccgc agccaggccg gcggcgacaa catctctaag
      301 agcgtcagcg gcacgggcaa gacgagagtc aaagtataca tcaacaattc gttagtacag
      361 gaccagtata tctaagggga ggaacatgac aaaaggacac gtcatcaaga actataacgg
      421 ctattattac gtcgatgtcg gccgggaagg gctcatcgag tgccgacgcc gcggcaaatt
      481 gctgaaagcg aagatcctcg tcggcgacga gctggaaatc accgagctgg gccaggacaa
      541 gggcgtcatc gaagccctgc tgccccggcg caaccagata cgccggccgg ccgtggccaa
      601 tatcgaccag ctcctggtca tcatggcggc caagtcgccg gatccgaacc agttcctcgt
      661 cgataagatg ctcatgacct gtgaatacgg cggcatccat ccgacgctgt gcttcaataa
      721 gtgcgacctg gaccgggaga cggccgaatc ctacaaggcc ttttatgaac gctgcggcta
      781 tgacgtctat ctcgtgtcgg ccaagaccgg cgaaggcctc gataccctgc gtgatctgct
      841 gccgcaccgc atgacggcct tttccggccc gtcgggcgtc ggcaagtcga gcctgctgtc
      901 ccagatcctg ggccgggaag acttgtccgt cggcgccgtc agcgagaaga tcaagcgcgg
      961 ccgccatacg acacgccatt cggaaatcat gcgcctcgac agcgatacgt atgtcgtcga
     1021 tacgccggga ttcagctctc tcgacttcga gcacctcgac ccgaaggaag tcctgcgcct
     1081 cttcccggac atgctgccct acagcggcgg ctgcaagttc tcgtcgtgtc tccatctgtc
     1141 cgaaccggaa tgtacggtca aacaggccgt cgctgacggc catatccaga aagaacggta
     1201 tgacacatac tgtaagatac tcacttccat tgcagaaagg aaacgatagt catggtaacc
     1261 atttgcccat ctatattatc ggcggacttc tcccgcctgg ctgaagacat tcaggacgtc
     1321 gaacggaagg gggcagattg tatccatctc gacttgatgg acggccattt cgtgccgaac
     1381 ctgacctttg gcgtccccgt cgtcaaggct ctgcgtagct gcaccaagct gcccttcgat
     1441 gcccatctca tggtagaaca tccggaaacc tacatcgacg gcctggctgc tgccggtgtc
     1501 cagtacgtca cggtccatca ggaagcctgc acccatctcg accgggtcct gcagcagatc
     1561 cacgaagccg gcatgaaagg cggcgtcgcc ctcaacccgg cgacccccgt atcgactttg
     1621 tcctgcctgg ccgggcagct ggacatgatc ctcatcatga gcgtcaaccc cggcttcggc
     1681 ggccagaagt tcattcctta tgccgtcgat aagatccgcc aggccaaggc gcttctggaa
     1741 caggccggca atacgacggc tgtcatcgaa gtcgatggcg gcgtcaatgc caagaccgtc
     1801 caggccgtca aagacgccgg tgcgaccttc ctcgtcgccg gcagtgccgt cttcggagct
     1861 gacgaccggg cagctgccat cgcgtccttg cgataaggac ttgtatttct tcttttcatg
     1921 tgctaaaatc atacatgtag ttcaaatgaa taaggatagt ctttgaggca aggtgagaaa
     1981 gcggcgcttt caattcctga ccggcggtga cagtccgcta acccgcaagg gttgaaccga
     2041 tgcaatttcg gtaccgacag tacagtctgg atgggaaaag acgacagatt gtgtttttgt
     2101 tgcgctcaaa aggctatggg aatcttacgt ccatagcctt tttttatctt gcgaatctga
     2161 atagaaggga aggaatacat tggaaccggc agagtatatg aaaaaagccc tggaattggc
     2221 aaagaccgcc ctgggctata cgacacccaa tcctgccgtc ggttgcgtca tcgtcaaaga
     2281 tggcgaaatc gtcggcacgg gataccatca taaggccggc acgcctcatg ccgaagtatg
     2341 ggcattgcgt gaagctggcg aacgggcgaa gggggctacg gtctatgtga ccctcgaacc
     2401 ttgcgcccat tacgggagaa cgccgccgtg tgcccggacc ctcggcgaca aaggcgtcgg
     2461 caacgtcgtc atggccatgc tcgaccccaa ccccttagtg gctggcaaag gcgcggccat
     2521 cctgcgcaaa gccggcatcg ccgtcgaagt cggcctcatg tcgaaggaag cggctcagct
     2581 caacgaagtc ttgatcaagc acatgatggt ccagcaaccg tgcatcccca tcaacctggg
     2641 ccccagcctc caaggccgca cagcttcacg cacggggacg gcccagtggc g
//
LOCUS       DAWCYS010000237         2691 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1904_length_2691_cov_4.153642, whole genome shotgun sequence.
ACCESSION   DAWCYS010000237 DAWCYS010000000
VERSION     DAWCYS010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_2691_cov_4.153642"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          676 a          823 c          713 g          479 t
ORIGIN      
        1 cgccggaaac cctcaagacc cgtccgcctg tcgtcaccat catgggccac gtcgcccacg
       61 gcaagacgag cctcctcgac gccatccgtc agaccaatgt caccagccac gaagacggcg
      121 gcattactca ggacatctgg tcttatcagg tcagctacca gtgcaagaaa atcaccttca
      181 tggatacgcc ggtccacgaa gctttcacgg ctatgcgtgc ccgcggtgcc caggtcacgg
      241 atatcgccgt cctcgtcgtc gcagctgatg acggtgtcat gccgcagact gtcgaatcca
      301 tcaaccatgc caaatcggct ggcgtcccca tcatcgtcgc tatcaacaag atcgataagg
      361 aaagcgccaa tccggaacac gtcatgcagc agctgacgga atacggcctg gtcagtgaag
      421 actggggcgg cgacaccatc atggtccccg tttcggcaca taagaaaatc ggcctcgacg
      481 atctcttgga aaatatcctc gtcctggcag aagtacagga tctcaaggcc aacccgaacc
      541 gtccggctca gggtgtcgtc atcgaagcca agctggataa aggccgcggc ccggtcgctt
      601 ccgtcctcat ccagaaaggg accctccgcg tcggcgatac gatcatcgtc ggcacctgct
      661 tcggcaaggt ccgcgccatg aacaacgaac ggggcgaacg cgtcaagaaa gcggaaccgt
      721 ccatcccagt cgaaatcctc ggcctcaccg acgtaccgga agccggcgac atcctcaacg
      781 tcaccgacga aaagacggcc cgttccgttg ccgaaaaacg cctggaaaag aaacgttcgt
      841 ccgcactcca cgtcaatgcc aaagtcaccc tggatgacct gttcaaccag atccagcagg
      901 gcgaactgaa ggaactgccc ctcatcatca aaggcgacgt ccagggttcc gtcgaagccg
      961 tgagccagtc cctcatgggc atcaagagcg acgaagtccg catctccatc gtccatgccg
     1021 gcgtcggcgc cattaacgaa tcggatatca tgctggcttc ggcatcgaac gccctgatca
     1081 tcggcttcaa cgtccgcccc gatgcggcag cccgcaagat ggcagaacat gaaaaagtcg
     1141 acatgcgcat gtaccgcgtc atttacgacg ccatcaacga cgtcgaagcg gctatcaaag
     1201 ggatgctgga aaagaaatac gaagaaaaga tcgtcggccg tgctgaagtc cgtaaagtca
     1261 ttacgacgcc gaaagtcatc gtcggcggtt cctatgtcaa ggaaggcaag atcaccagca
     1321 cgtccaagat acgcctcatc cgcaacggca tcgtcatcca cgaaggcgaa atcgacggcc
     1381 tgcgccgttt caaagatgat gtcaaggaag tcgcagccgg ctacgaatgc ggcattaccc
     1441 tggaaaaata ccgcgacatc aaggaaggcg acgaaatcga agcctacgaa atggtcgaag
     1501 tcgaacccga ataaagggag gtacgtacta tgtgagaatt acgagtacgc aagatacagg
     1561 aatttatcaa acaggaagta tcgaagatgc tcctgaatga actgaaagat ccgcgcatcg
     1621 gtttcgtcac cgtcaccgat gcccgcatca ccggcgacct gcgcgatgcg acagtctacg
     1681 tcagcctttt cggcagcgac gaagccaagc aggaaatgct gaaagccctg aagagcgcga
     1741 ccggctatat ccgcacggaa gtcggcaagc gcctgggtat ccgctattcg ccgaccattg
     1801 tgtttaaaga agatacatcc ctcgattacg ggatgaagat cgataagatt cttcgcgaca
     1861 ttgaaaagaa ggactgatac ccatgaattg ttcggctcag gaaatctata ccttactgcg
     1921 ccattacgat aagatcctgc tgacagccca tgtcagtccc gatggcgacg ccatcggcag
     1981 cctcctggcc ttgtggcact ggctgcagct gcaggggaag aaggccgtca tggtcatcga
     2041 cgacgatatc gacgaccgct atgacttcct cgacggcctg cagtacatca acaagccgga
     2101 gcaggtcgaa acggatgatt cctggctgac cgtcgtcctc gatgcgacgg gcctcggccg
     2161 catcggccag gctgaaaagc tggtccgcgg caaggtcctc aacatcgacc accatatttc
     2221 caacgagcat ttcgcccagt gggaatacgt ccggcccgac tgcgcggcga caggcgaaat
     2281 cctgacctat ctcttcgacc tctggcagcg gggctggaac acggccatgg ccgatgccct
     2341 ctatacggct atcgccacgg actgcggctt cttcaagttc aacaacacga caggccatac
     2401 gctgcgcatg gcagccctcc tcgtcgacca cggcgcccgg cccgatttca tttcggaaca
     2461 cctcgacgcc cggtccctgg aaaaactgaa ggccctgtcc aaggtgctgg aacacctgga
     2521 gctctttgat gacggccgca tttcctgcct gtcctacacg ccggaagtcc tcaagatcac
     2581 tggcgaacac acggggatgt acatggatta cgcccgcaac gtcaaagggt cggaagtggc
     2641 ttttacggtc aaatacgtca gcgataagga aacgcgggtc agcctgcgct c
//
LOCUS       DAWCYS010000238         2689 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1906_length_2689_cov_5.778284, whole genome shotgun sequence.
ACCESSION   DAWCYS010000238 DAWCYS010000000
VERSION     DAWCYS010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1906_length_2689_cov_5.778284"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          478 a          771 c          630 g          810 t
ORIGIN      
        1 agtttttata atattgggcc tttcggttga ggcacctttg cttcatggct ttccagcacc
       61 aatatatttc tttatgcttt tgcctaatcg ttggcatcgt tattgccccc aataatcttt
      121 cctttgttat ctaattcgct ccataaaaat gaacctttac cacttccgcg ccattgccca
      181 atgccgtgat agcgtccata gtcaagccat tcacgcactg ccgcttcata tgcatcagac
      241 agaactacta ccgtaaatgt aagtttgcta ccggctggca cgctttcact gtttgccagc
      301 gccacgcgtt cgccctgtgc ggtctgcgct ctcaacggcc gctggcagtc gccgatttca
      361 gcgccttccg gcatttcaat catgattttc cgcggttcga caaagataca gccgtcgatg
      421 actttcttgt aagccttaat tttattcgta tgggcggcga atttttcgcc cttgcaacgc
      481 tgtaagaatc cggcggccgc tttgaagaac ccgcggattt ggtacgagta gacgaacggc
      541 ttgccgtctt ccttcgggaa aatcgtcttg cccttttcga tgacggcttc cacgcccagc
      601 gcttcgcgtt cttcttcccg gctgccggca tccggtgctt tcgacgcgat gaattcttca
      661 tgaatcttcg ggtcgctgtt cgccgtgccc aacatttctt cgatgagttc gatagttacc
      721 tgcaatttct tcatttgttt ttcctcctgt tatccctgta aaagttatct ggctccacgg
      781 ttgccgtggt atatatcgac tggcccgttc tgcttctcat acgcttccag tgccgggtaa
      841 tctatattgc actctacatt gaggcatttc atgaattccc agataggcac ttcgttatga
      901 gtcagcacgt agaccttatc tttgtagtag ttctgcaaat ccttgaggcg tttgtcacgc
      961 catttatatt tcgtgtacag cgtccagaat atgacgatgg ctaccccggc tatcatgtcg
     1021 cgtgtcttcg ccatgagttt cagatcgcgc gtccggccgg ttatcatttt ctgcatccgt
     1081 tcggcgatac gctcatttaa gaaccggtcg aatccatgtt cacatagttc gtcgcgccag
     1141 tcgtaaatga agccttcctg gaattcgtcg agatgctccc acatgtcgtt catcgcggcg
     1201 aatcggttgc ggccgaatcc gtatttatcg tgaaggcagc tgaacaataa tgtcgttgcc
     1261 cagtccgtac cgctttcggc cccggcttca cgccgtactt ccatgcgctg cctgtcctgt
     1321 ttcgccaggg cgcggctgat agcgttcatc gtatcagccc tttcttgcgg accatgtaag
     1381 cgccgtatga caatcccgct ttacgggcgg ccgcaatgtc atcatcgagt cccgtccgga
     1441 gtcggctttc aaaggccgtt tctacagcct tgtctctcgc ccttttcatg cggttgcgcg
     1501 cctggataat cgttttcggt cgcatgatct ggtagtaaat cttcttccac gcgtcgcggc
     1561 aatccgggca tacgctgaat ccactcatca tgggcttgtc gcacacgatg caggtaggcc
     1621 ggcgtgtata gccctgtttt cggtaggcgt cccaatccca atagcgcttc gtgaattcat
     1681 cgaggcagga ctggcacaac cgctgtgctt tatagtacgg gtcgaattca tccccgcaca
     1741 ccatgcattt gctcatcttg ctgctcctct cttcatgacg atggtcatgc tcacaagccc
     1801 cgtgttcggg tccagccaga accgcatgta ttcgttaccc atgcgcatgt tgcaggccat
     1861 cccttccttg aaggcgggga cgttcttgat aatcagttcg tccgggattc cgggaaccgc
     1921 ccggcgggcg aattcgatga tgtctttcgg cacgtcgtcc agcgacggcg gcttcttttc
     1981 cttgctcggc cgcttcgtcc actggtgtct aaggccgctt atcatgtcgt ggatgcgctt
     2041 gcgcccggtt tcgttgatcg gcgcttccgg aagtgccggg tagtccggca cggcgttgat
     2101 tttccgcttt acttctttct tgatcatcgt cacgctcggc atggagtcgg cgctgtcaat
     2161 cacacgcccc acggcccgca ggacgtcgcc gtcgctgtag cgttcaaact ggagcatcat
     2221 cgcgccgatg aattcatcgc cattctggcc actcagcttc caggcgtcat ttggataaga
     2281 gcctcttaat atcttcaaaa cttgattggc tgtgttgctc gtcatactct ttgcacatcc
     2341 tttctctccg tgcgtcatct tcccgcagcc gttcgtcgcg ggtcttataa cgccctttat
     2401 atggtgtcga agggctttcc cgtttttccc aggtgcgtac cgtggctttc cagtccttca
     2461 tgtgggtgtt tcctctcatc catccgttag cctcgtaatg atcatagaag tattccgggt
     2521 caatgttgtt tccacgctct tcacaatacg cacggatttc ttctaccgtg ggctttacaa
     2581 aacgtcgttc cttgtgtttt ttagtcgtgg gcgcggcgga aggtttactt ccgctatcta
     2641 tactttcagt cttacttaat tcagtcttac ttaattcagt cttattagc
//
LOCUS       DAWCYS010000239         2685 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1914_length_2685_cov_4.587833, whole genome shotgun sequence.
ACCESSION   DAWCYS010000239 DAWCYS010000000
VERSION     DAWCYS010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_2685_cov_4.587833"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          647 a          775 c          743 g          520 t
ORIGIN      
        1 gatacaaaag taacggattc gcgggctacc gatgacggca aggccatccg tcgccgacgc
       61 gaatgccagc agtgcggccg ccgtttcacg acgtatgaaa tggtcgaaga agtgccgctc
      121 gtcgtcgtca agaaagatgg ccgtcatgaa ttattcgacc gcaacaaatt attgaacggc
      181 ctgctccggg cctgcaacaa gcgggaagtc acgcggcagc agctggagga catcgtcacc
      241 aaggtcgagc gtaacatccg caataccctg gcccaggaag tgtccagtga aaagataggg
      301 gaaatggtcc tcagcgagct gcgctctgtc gatgaagtcg cttatatcag cgttgcttcc
      361 ttataccgcg aattcgccgc tctggacagt ttcatggctg aactgcagca tctcatgggc
      421 acgaaagaaa agaaataaga gaccggaatc gaggaacatt atgacagaaa attatatcga
      481 tcattttttg aaccatgtcc tgccggaatt gcgcatcgcc gtcatcggcg acatcatggt
      541 cgaccgctat gtgttcggta aagtggaacg catttcgccg gaagcgcccg tacctgtcaa
      601 taaagtcgac cacatgacgt ccgtcctggg cggggctgcc aacgtagcct cgaacctggc
      661 caatctcgat tgtcacgtct ttctgggcgg cctcatcggc gacgatgaaa acgcgtctct
      721 cctgcaccag ctgctggaag acgcccatat cgacgggtcg ggcctggtcg tgcgccagga
      781 ccacgcgacg acgacgaaaa tgcgcatcct cggcgcccgc cagcagatgg tacgcctgga
      841 ttttgaagaa gtgacgccct tggccgttac ggaagaggaa caactcatga cctggctgta
      901 caactgcgtg gcagccggcc tggacggcat catcctgtcg gactataaga aaggcgtcct
      961 caccgattcc ctggctcaga gcatcatcaa agaagccaat cgggccggga ttcccgtcct
     1021 ggtcgacccc aagggcagcg attggacgaa atacaacggc gccgatttcg tgacgccgaa
     1081 catgaaagaa ctgagcgact gcctgggacg gcctgtagcc aatgaaggac aggccgtcgt
     1141 agcggcagcg gaaatcctgc atgagcagta tcagttcgcc cacctcatgg tgacccgctc
     1201 ggaaaagggc atcaccgtca tcggccggga cggcaaggtg tggaacaacc cggcgacggc
     1261 gcaggaagtc ttcgacgtat ccggtgccgg tgataccgta gccgcagctt tcctgtcggc
     1321 tattgccggc cacttgtcga ttcgcacgag cctgcagatc gccaacgccg cagcgggtat
     1381 cgtcgtcacc aaggtcggga cctatcccat ccatcgccac gaattgctca aattatggca
     1441 ggaatggcag ccggcccact ggcatccgta tcagcccctg agtcgggaag aagcggctga
     1501 aaagatacgc caatggcaga agaagggcga taccgtcgtc tttaccaatg gctgcttcga
     1561 catcctgcac cgcggccacg tcctctacct gcagcaggcg gccatgctgg gccagcacct
     1621 cgtcgtcggc ctcaactccg atgcgtccgt gcggcgcctg aaaggggaaa cgcggcccct
     1681 ggtcaaacag gaagaccggg ccatcctttt gagtgccctg gcctgcgtcg atgaagtggt
     1741 catcttcgag gaagatacac cgaaagaact tttacaggtc ctgcgtccgg atatcctggt
     1801 caaaggcggc gattacaaag ctgatgaagt cgtcggccgg gaatcggtca agcgcgtcga
     1861 gatcatttcc tttgaagacg gatattcgac gacaggcctg atccagaaaa tagctgattt
     1921 agtaaagaag gggaaactat gaaaattgta acaggcggtg ccggttttat cggcagcaat
     1981 atcgtaaaac agttgaacaa ccggggcatc aacgacatcc tcatcgtcga cgacctgacg
     2041 gacggccgca aatgccgcaa cctccagggc ctcgatttct ttgattacat ggactatgcc
     2101 gacttcgacg aacagatggc cgacgacacc ttcgactgcg gctacatcga cgtcgtcttc
     2161 catgaagggg cctgctcgga cacgatgaac tacgacggcc gctacatgat gaagaacaac
     2221 tatgaaggca gcaagaatct cctgcgctac tgcatgcagc gcaagattcc cttcctctac
     2281 gcttcgtcgg cttcgactta tggcagcggc aagcacggct tccgcgaaga cccggcctgc
     2341 gaacaggcgc tgaatcccta tgcctattcc aagctccagt tcgaccgctt cgtccgccgc
     2401 atcatgcccc tggccaagag ccagattgtc ggcttccgct atttcaatgt cttcggtccc
     2461 caggaaaacc acaaggaccg catggcttcg ctcatctacc agaagtacca tgaactccag
     2521 gaaaaaggga agattaccct ctttgaaggg acggcaggct atgaaaatgg cggccagatc
     2581 cgcgatttca tctacgtcaa cgacgtagtc aacgtaatct tctcttgcgg ggaccatccg
     2641 tatctgtcag ggatctacaa ctgcggctcc ggcatgggcc atacg
//
LOCUS       DAWCYS010000240         2682 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1918_length_2682_cov_4.123715, whole genome shotgun sequence.
ACCESSION   DAWCYS010000240 DAWCYS010000000
VERSION     DAWCYS010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_2682_cov_4.123715"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          711 a          758 c          714 g          499 t
ORIGIN      
        1 ctgacgccgg cagccgacct cgatccggtc cacctggcag ggacgacaga caaacgtgcg
       61 acgctgcaca atgaggatta catcaaagaa aaagacatcc gcataggcga taccgtcgtc
      121 atccagaagg ccggcgaaat catccccgaa gtcgtccgct ccctgccgga actgcgcgac
      181 ggctcggaaa aagtcttcgc catgccgacg acttgtcctg cctgcggcag ccctgtcgtc
      241 cgccgcgatg gggaagcggc ctggcgctgt accaatccgg actgcccggc tgtcatcggt
      301 gaaaaaatcg cccatttcgt ctcccgcgat gccatgaaca tcgacggcct gggcgacgcc
      361 atcgtccagc agctcctggc ccagcatctg atccacgacg tagccgacat ctacggcctg
      421 actaaagaag aactggtcga cctcgaaggc tttggcgaca aatcggcaga caacctgctc
      481 aaagccatcg aaacgagcaa atccgtcggc ctggcccggg tcctctttgc cctgggcatc
      541 cgcttcgtcg gcgccaaagc cggccgcgtc ctggccgaat ccttcggctc cgtcgatgcc
      601 ctcatgaaag ccaatgccga tgacctgaca gctatcgacg acatcggacc gcgcattgcc
      661 gacagcatcg tagcctactt cagcgacgcc aagaaccagg ccctcatcga aaaactgcgc
      721 gctcacggcg tcgatatgac ggcagaaaag aaaacactca tcaatacgac ctttgacggc
      781 gaaatcgtcg tcctcaccgg caaattgcag atgttcagcc gcaaagaagc cgaacaggcc
      841 gtcgaagcac gaggtggtaa atgcacgtca tcggtcacca aaaagacgac ccttgttgtc
      901 gtcggagccg acccgggcag caaatacgaa aacgccaaca aactggggac ccccatcatc
      961 agcgaagccg aattcaaaga atggctcgag cgctaaataa gccacgtctc agtcagcaga
     1021 gctgccagct cacctacaaa tggagcctta agcctcccct cataggccct ttttggcccg
     1081 tcaggtgctg accgtaggga agcgcagggc ggttgccaaa acggagagcc gagctggagg
     1141 tggcccaacg ggccggagag gttgcttgta ctttcgcagg ccgccggtcg cagttcgcag
     1201 ttcgcaggcc gcctcggact cagatgccgc tgtgcggcga cagactcaga ctaacaacga
     1261 cgcttcggga aaccctccgc gcgtagcggg ccgccctctg ggacggcccc tacggatgcc
     1321 gcggcgcggc gacagtagaa atgcccccgg ctggggcgta ggggtcgccg cgtgggcgac
     1381 ccgccacaga acgaatggga atacctaaac agactgaatg gagatgattt tatgcgtgct
     1441 atggataaag taatgaaaga attacatgca tttattgcta agaaggctcc gaacggtgtt
     1501 aagagcgaag aagagatgca gcagattatt gaagaattca tgatgcagca taatgaggct
     1561 gtccaacact tgcaggactt tttgcaggag aatgggcagg acgatacagt accagccgat
     1621 gtctatgatt atttagactt agctgagcag gcgagccgaa aaaaagacaa acgggaatat
     1681 ttggctaaag cagccgaact ggagccggat aatgtggaag tcaaactggc ccaagctgaa
     1741 ttggattcga aaggccccct ggatatgctt gaaatccttc cgggcatgat tgcagccgaa
     1801 gaaaaacggt taaaagatca ggaaatatac cagcgtagca aaggtgattt ttggctggac
     1861 tttgaaacac ggccttatat gatgctgctt caagaatatc tatcggatct gactgaatgt
     1921 ggtatctaca acaaagccat ccagataggg gaagaaatgc ttcgcctcaa tcaaaacgac
     1981 aacttgggaa tccgttttct gctgatgccg ctgtatgcta aaatgtgcaa tgaaatgaag
     2041 gctttgaaac tgtataaacg gggaaaatac gaacaaaaga gttcctttta tctcttggcc
     2101 ttagccttgt tgtacttcaa actcggcgat tggcccgagg cgaaatcata cttggaaacc
     2161 ttgaagcagg aataccctat tacgaaaaag ctcatccgtt ccattaaaaa gggtgaccag
     2221 agcatcttcc agccctacct tgacaacccg ccatacgccc cattttccga tgaagaactc
     2281 atcactgcct atctgacgaa ctatgccatt tacgcaaccg aaccgtactt cttcgactgg
     2341 gccgacgaag ctctcatcgt ccatcgtaaa aagaagaaat aatcggaatc aaatgccgct
     2401 gcgcggcaac agattcaaag tgacaacgac gcttcgggaa accctcagtg cgtagcgggc
     2461 cgccctctgg gacggcccct acggatgcgg cgtcccgccg cgacaaacgc aaaccaacaa
     2521 cgacgactcg cgtaatcctc agctggtatc gtaaaaaaat gccccttgcc ggggccttcg
     2581 taggggacgg ccaagccttg ccagcccgtc aggtgctgac caacgagaag cgcaggacgg
     2641 ttgctggaac ggacagctgt ggctgggcgt cccgctcaga at
//
LOCUS       DAWCYS010000241         2672 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1925_length_2672_cov_3.948796, whole genome shotgun sequence.
ACCESSION   DAWCYS010000241 DAWCYS010000000
VERSION     DAWCYS010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_2672_cov_3.948796"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          600 a          789 c          762 g          521 t
ORIGIN      
        1 ctctatatcg gttatgggga gtttatccat gcgtaccaca cgtcggtcgt cgtcgaccgg
       61 gtacggcgct ggctgccccg gattgtgggc ttttatgatt gggtgggaga taagacatga
      121 ttgacatcat tttagtaaaa aacatatttg acgaccatcc aaagacggag gtccagtcta
      181 aagattatat cgccggcatg acgctggcag gctatgtcga catgcgcgat aaggacgcct
      241 acgtcggtgg tatgtgggtc cgcgacccga agatctttta cgtcaaagac ggtatgcagc
      301 tcctcatcct gccgcacgta gcgggtggtg gcttcaaaaa gattttgggc accgtcctta
      361 tggtcggcct catgatcgca gcgccggcca tgtttgtcgg ctggtcgtcc ctggctttgc
      421 gtgcgctggc gtccggcgct gtcatgatcc ttggcgggaa aatcatcaac agtattttcc
      481 acctcaacca ggcggcatcc cgcggcattg acgaccagag ctcaccgacg tatggctggg
      541 aactgccgaa cgtacagacc caagaaggcg gcgtcatcgg cgagacgttt ggtgagtgta
      601 tccctgcgcc gcagctcctt atgtatcacg tcgagacgac gaacagcgat gaccaggaca
      661 agaacaacca gtacttgaac gtcttgtact gtggtggcta cgggccggtc gaaagcatcg
      721 atgacatccg catcgggcac acgcccatcg agaactttag cgacgtgcag attgaaacac
      781 gtaaagggac gaacaaccag tccccgatac cgtttttcgg cagcaccgtc gccgaccagt
      841 tcgtcgacat gaccctggct gagaataagc ctgtcgtacg ctcgacggac atggagaact
      901 gcaaccgcat cgatgtgacc gtgtcctggc cgtccgggct ctatcacatg aacgacgacg
      961 ggaactatga aggctgcacc gcgcggttcc gcatcgagta tcggaagacc gggacgtcgg
     1021 actggcgcgg cggcaatatg tacgaagcgc actcttcgtc cagccttatc agtaatgtaa
     1081 ccgccggggc aaaagcggac cgcgatgaag tgtggaccat agcccggcag gacactacga
     1141 cgcgtgtgaa agtgcgtact cactatcgca ccgagactac ctggaagtgg gtcatccgga
     1201 gcaataagcg ctctgaagtc ttcgacctgc cagcgttgaa aaagggtgac cgctatgata
     1261 acggctacat cgcatttact cgcgaactcg tgttcagctt ccttgatgga ttcagcctgg
     1321 atggggtaca gatctacgtc cagaaatgcg actactacat cacgaaggcg acgaacagcg
     1381 gcgtacggcg ttcatacgct atcaaaggcc tggagccggc ccgctacgat gtgcgggtca
     1441 cagccctcag tctgcctcac acgtcccggg actgctcttt tatgcagtgg aacacgctgt
     1501 cgtcctttgc cgacgacagc gcctacagcc gtccgaacaa ggtcctcgtg gccctgcgca
     1561 tcaaggccac gaaccagctc aacggcggcg tgccggacat caactggcgc cagcgccgca
     1621 ctatcggcta cgtctggaac ccggaaacgc aggcttacga acaagtggac ctgcgcaacc
     1681 cggtgtgggc cgcgtacgac atcctgcatc actgccggaa gctctttaac gtcaacacgc
     1741 agaccgaaga gtatcatgtc gatggctgcc cgaaggaacg ctttacgcaa tactgggacc
     1801 agtggcaggc cgcggcggac tatgctgacg aggatgtgaa gaacgctgac ggctcgacag
     1861 agccccgctt ccgcttcgac gcgtactatg gcacgtccct gcgccggtac gaagctgcga
     1921 acaaggccct ggccgttgct catgcaacgt tggtgcgcca tggtctccag ctcggtgttg
     1981 tcgtcgataa gcccggtgtc atccaccagg tcttcggtga aggacggacg ctcatctcct
     2041 cggtaaaagg cgagttctcc gcgaccgagg accgggccca cagcttacat gtcacgtaca
     2101 acgacacaca gaacgacttc aagaatacgg aattcttcat ccgctccgac gcctatgcct
     2161 cggccgaagc gaatggccag gacaatacag cgcaactgtc tttgttcggt gtctccagcc
     2221 ggacacaggc gtaccgcgaa gccgtctacg ccctggccac caacgaacgg cagctacagg
     2281 ccatccagtt ctcggctgac gtcaacgctt ttgtctgcga gtacggcgac ctcatcggcg
     2341 tcaactcaca ggtaccgcgt atcggcgtgg ccagtggccg catcgtgtcg gtgtccggca
     2401 cgacggtcaa gctcgacaag actgtcacct ttaccaaggg ggaagcgtac accgtcatgt
     2461 tccagctgtc gactaatgac gcgctgatca gccgcgaagt gaccgctttc acggcggaca
     2521 cgacgaccga tacggttacc gtcacggcag cttttgatga cggcgcggtt ccagtcgccc
     2581 tcgacaacta tgtactgggc atcaaggaca aggccgcaaa gcctttccgc atcacgaaaa
     2641 tcgagcgcga cggcgaccag aaggtcagca tc
//
LOCUS       DAWCYS010000242         2667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1929_length_2667_cov_5.104135, whole genome shotgun sequence.
ACCESSION   DAWCYS010000242 DAWCYS010000000
VERSION     DAWCYS010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_2667_cov_5.104135"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          616 a          739 c          700 g          612 t
ORIGIN      
        1 gctcatctat ttggcctttg tctttcccaa gctggccagc gacgcctctt tttcccgcga
       61 cgaattgccc aagctgctca agaatcccgt cgtcatcagc gtctatatcc tgtccgtcct
      121 gttcgcgacg gcctatttta cagcgtacag ctatatcgag ccctttctca agaccgttgc
      181 tgcctttagc gaccagggca tcacgtcgac gctcatgctc cttggcgtca gcggcctcct
      241 ggggagtttc gtcttttccc ggttttacaa tcatttccgc tatggcgtca tccgggctgg
      301 tctgttgggc ctggtcatcc cgttcctcct gtggcaggcc gctgcgggca gggcagggac
      361 gatggtcgcc gcctgcctgg tcgtcggctg tgcgtcgacg ctgttcaacg tcaccttcca
      421 ggccgaactc atccgccacg tcccgatggg ggcagccccg gtggccatgt ccatcttttc
      481 aggcatcttc aacgtcggta tcggcggcgg gacctggatt ggcgggcagg tcacggccca
      541 tggcctgcta ccgtatatcg gctatgccgg cgcagccatc ggcttagtcg ctgccgtgta
      601 ttgctttacg gcctatttcc atttcctggg cagggccgga gaaagtcatc aaaaaaattc
      661 gtaaaaaaga cttgcatttt tctatctctt caagtataat attaattgtt ccgattcgga
      721 acgagcttga agtgatattc ctcaatagct cagttggtag agcaatcggc tgttaaccga
      781 tttgtcgtag gttcgagtcc tacttgagga gccatggccc cttggtcaag cggtctaaga
      841 catcgccctt tcacggcgag aacatgggtt caaatcccgt aggggtcacc atatgaaaag
      901 gtcctgtcgc atatgcgaca ggaccttttg ttgttcgttg ttcgtggcta gtgattagca
      961 aagtgcttac gagttactaa cggctgacgg ctgactgcta actgctgact gcttacggtt
     1021 gactgctgat tacgagtcac gaagaaactg ccataaagga agcttcttcc tttcgcatcg
     1081 ttcgatgtcg gcttatgcaa aataagcaat gatgatttct ggttcagtaa acatgagtct
     1141 ctttataatg gacaaatacg cgaatactct attgacaatg gacagataaa ggcgtatagt
     1201 aaaggcataa gcaaaccgta tttattctaa ctaacgaagg agggacgctg ctatttagca
     1261 gcacatacca tgagtgaaga aaaagtaacc ttacaaatga tcaaagatgc tcaggaacgc
     1321 ttgaagggcg tcgcccagaa gacaggcttg tcgtacagca attccgtcag cgatctggct
     1381 ggctgcgaag tctatttgaa gctggaaaac ctccagcgga caggctcctt taaactgcgc
     1441 ggtgcctata acaaagtcgc atccctgaca ccggaagaac gggaaaaagg cgtcatcgcc
     1501 gcttcggccg gcaaccatgc ccagggcgtt gccctggcag ccagcgaata cagctgccag
     1561 tccgtcatct gcatgccgaa acacgctccc ttgagcaaga ttacggcaac ccgcggctac
     1621 ggtgccaacg tcgtcctcta cggggacttc tttgacgaag ccgctgccaa agccgttgaa
     1681 ttgacgacgg aacacggcta caccttcgtt catcccttca atgacccgca agtcatcgcc
     1741 ggccagggga cgattgccct ggaaatcatc gaacagctgc ctgaagtcga cgccatcgtt
     1801 gcaccgattg gcggcggcgg cctcatttcc ggcctggccg tagcagccaa gagcgtcaat
     1861 ccgaagatta aagtcatcgg cgtccagacg gaaaacatgc cgtccatgaa ggaatccgtc
     1921 gaaaaggggc aggtcatcac ctacaacggc aaggctaccc tggccgatgg catcgccgtc
     1981 aagacaccgg gcgacctgac ttttgatatc tgcaagaaat acgtcgatga aatcgttact
     2041 gtcgatgaat cggaaatcgc cagcgccatc ctgctcctcc tcgaacgggg caagaccgtt
     2101 gccgaagggg ctggtgccgt accggtagca gccctcatga gcggcaagat ttccggcatc
     2161 cgcaacaaga aagtcgcagc cctcgtcagc ggcggcaaca tcgacgtcaa taatatgacc
     2221 cgcgtcatca accagggcct gatcaagtcc cagcgcaaga ttttcttcca gaccgttatc
     2281 cccgacgtac cgggcgaact ggtcaagctc ctgaccctta ttgccggcac caatgccaac
     2341 gtcctttcca tcacccatga acgcagccag cacggcatcg acatgggcat gaccgccgtt
     2401 tcactggaac tggaaacggc caacgaaaag cacgttgaaa aattgatgac catgctcaaa
     2461 gaccatcatt attttgttac cttgaagtag tggaggtctg atcatggaac agcagaaaat
     2521 ggaacgcggc ctgaagccgc gtcacgtaga gatgatcgct cttgggggga cgattggtgt
     2581 cggcttgttc atggggtcgg ccaacaccat tcagatggcg ggaccgtcgg gagtgctctg
     2641 ttatgccgta acgggggtcg tcatgtt
//
LOCUS       DAWCYS010000243         2649 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1941_length_2649_cov_5.077487, whole genome shotgun sequence.
ACCESSION   DAWCYS010000243 DAWCYS010000000
VERSION     DAWCYS010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_2649_cov_5.077487"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          553 a          760 c          738 g          598 t
ORIGIN      
        1 atcatagccc ggccccgtta cttgcggatg ccgacgcgga gcttcgcact gcgggcccgc
       61 ggattcgtat ccagttcttc ctgcgacggc aggatgggct ggcgcttcgt ttttaggatg
      121 gcatgatgat ggcacgtaca aacgggcatg tccggcgggc agatacaatc tgtcgccaag
      181 agcctcaacg tctgtttggc aatgcggtct tccagggaat ggaaggtaat gacgcacagc
      241 cgtccgcccg gcttcaggcg ctgggcagcc tgttccaggg aatcgtgaag gattcccagt
      301 tcatcattga cttcgatacg gatggcctgg aacgtccgtt tggccggatg gggtccgtcc
      361 tttcgggcac cggccgggat ggccttcttg atgacctgga ccagttcttc tgtccgttcc
      421 aagggctttt cccgccgcgc ctcgacgatg aaggccgcaa tgcgcttggc ccaccgttct
      481 tcgccgtagg tcttgatgat gcggtataag tcggcctggc tgtagccatt gacgatatcg
      541 taagcgctgc gcttctggtc ccggtccatg cgcatgtcca ggggaccgtc ctgcatgtag
      601 gaaaagcccc gttccggcgt atcgagctga tagctggaca cgccgaggtc gaacatgacg
      661 ccgtccacct tttctatccc caattcatcg agcacggcgg gcagctagcg gcaagtccgc
      721 tgtaccagga tacgccggca ggccgtatcc ttcagccgtt ccgtcgccgc tgcaatggcg
      781 tcccggtcct ggtcgaggcc gatgagcgtc gctttaggcg acaagcgttc ggccagcgct
      841 cgggaatgac caccgccgcc gagtgtacaa tcgacataga tcccgtcagg gtcggtaatt
      901 aagttatcta tggtttcttt acgaagcaca ctgacgtgat gaaaattcat aattcctcct
      961 aaaagtccaa gtccagggca tcgtcgagac tttccgcaat ttcttccatg accggtacgg
     1021 tcttggcagc atacgcttca taagcgtctt tgctccagat ttcgacctta tcccctgtcc
     1081 ccaggacagt cacatccttg accagcttgg cataggccct caatgtctcc ggaatcagga
     1141 ttcggccttg gttatcgaat tatacttcag cagcactgcc gaagacaaac cgattgaagg
     1201 ctcggtcatt ttctttagaa gcccggagtt ttttcaaact gccggccaac cgcatccacg
     1261 cctccatcgt atagacagaa aggcagcctt ccaaaccgcg ggtaacgacg aactttggcc
     1321 ctagttcgtc ccgaaatttg acaggcaaaa taacgcgacc ttttgcatcg atggtatgtt
     1381 catattcacc cataaacata aggccgcaac tcctttcact tttcaccaca ttttaccaca
     1441 tttccacacg ccgtgacaac ataatggcga aaaaaagaag ttttgcctga aagtttcttc
     1501 tttcagacaa aacttcttgg ggagattcgt ggttcgtagt tcgtagtcca agttaaaagt
     1561 ttgcagtttg aagtttgtag tgaatagttt taaatttaaa gccatcttct acaaactccg
     1621 tactacaaac tatagagaaa agtaacttcg cataatgaca ggaacctgag tgcgtctcct
     1681 ctggcgggcc gccctttggg cggcccctac agcatgttta cgacgatggg ctcggtgcgc
     1741 aatttgtgga aatcgcagcg ccggacaagt tcatcccggc tatagcggcg ccgttccggc
     1801 accaggccgc cggcgtaggc caggtaggat aagatgtctt ctgccgtaag gccccggtcc
     1861 cgcaaggagc ggatagtgat gccgtgctgg cgcttggata ggcggttccc gacttcatcg
     1921 acgagcatgg gcacgtgggt gtatacgggc ggcgtataac ccaacgtttc catgagccat
     1981 atctgctggg ccgtcgacga taacaggtct tcgccgcgca ggacatgggt gatggccatg
     2041 gccccgtcgt cgacggagac ggccagctga taggcataca tgtcgtcggc ccggcggacg
     2101 acgaagtcgc cgcagcagcg cgtcaagtct tcgtggaacg gcccgtagac gccgtctgta
     2161 aaggaaatgg tccgttccgg cacatggacc cgccacgacg gcttcttggt catctgcttc
     2221 cgctgggctt cgctcaggcc gtagcaatgg ccgtcgtaga cggtatgctc gccggcatga
     2281 ggggcgccga cggcctgcag gcgggcgcgg ctgcaataac agggatacaa gaggccggcg
     2341 gttttgagtg tatccagggc cgccgcatag cggtcgtatc gttcctgctg gatgtacggc
     2401 ccgtaaggtc cgcctatgtc aggcccttca tcccaatcga ggcccagcca gtgcaggtct
     2461 tccatgaggg cctgggtaaa gatgggtttc gatcgctgtt catcaatatc ttcgatccgc
     2521 aggaccaagg tcccgccggc ctggcgcact tgcagccagg ccaggaaagc cgaccagaca
     2581 tttcccaaat ggatatagcc cgtcgggctc ggcgcaaaac gtccgcgcat gacactcaca
     2641 tcctaatcg
//
LOCUS       DAWCYS010000244         2633 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1956_length_2633_cov_3.884407, whole genome shotgun sequence.
ACCESSION   DAWCYS010000244 DAWCYS010000000
VERSION     DAWCYS010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_2633_cov_3.884407"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          704 a          609 c          501 g          819 t
ORIGIN      
        1 attacgatat ttactggcgg atgaaatgaa tttgcgaaag aatcttgaca ctacccacgc
       61 aataaacgaa caaacattct gggttcaata tatcatatct agggcaaatt agcaagatat
      121 ccattggtct ctcgtaatgc ccagaaattt gaaaaagcca cgaacctaga aagttcatgg
      181 cttttaccgc attacctata ctgatttaga taagtccgaa aagtatcttt taattcagga
      241 cgccgcaagg catattctac tgttgctttg aggtaatcca atttattgcc gatgtcgtag
      301 cgttttcctt tgatttcgca cgtgtagacg gcttggcggc tggccatgag ttttagggcg
      361 tcggataatt tgattttcct tttttattag gttggacacg gcgcagtatt tcaaagatgt
      421 ccggtgtcag gacatagcgt cccagcatgg ctgattttcc ctcggtttca tgaccgactg
      481 ctttttctgc accgaggaaa cgggaaactc cctcttcgtc cgtagcgaca ctttgaatga
      541 tattccgcgg aacgacctgt gcttccttca atgtctggca tcccaccata gtggcaccgc
      601 atttattata cgttcgcatc agctggtgta tggcgggttc cggcgcatga tagatgatat
      661 catcgccgag gagtacggca aagggttcgt tatcgatgaa ggattcagca caaaggatag
      721 catcccccac gccacgtata tgtttctggc gaatgtaatg aatatttatc ttggagattt
      781 cctgtaccat gtccagcatc gctactttcc catgctggcg cagatggtat tctaaatcta
      841 cattggtgtc gaaatggtcc tcgatagccc gtttcccgtg accagtgatg atcaaaattt
      901 cttcgatacc actttgaatg gcttcttcta cgacatactg gatgacaggc ttgtctacaa
      961 tgggaatcat ctctttaggg attgatttag tcgccggcaa aaagcgcgtt ccataaccgg
     1021 ccgcggggat aacggcttta cgaatgggct tcatattttt tcaccatctc cagagcactg
     1081 gcaatgacat catccatatc atagtacttg tattccgcta agcggccacc aaagatgacg
     1141 tttttttctt tcgctgctaa ggctgcatac tgttcatata aatgctgatt ccgttcgttg
     1201 ttgaccggat aataggcttc ttcgccggga tgccaatctg ccgggtattc tttcgtaaca
     1261 taagtaacag gctgttcgcc gaattcaaaa tgcttatgtt caatgctgcg cgtatacggc
     1321 gtttcccgat ccgtatagtt cataacagct acgccttgat aattttcttc ttccagacgt
     1381 tccgtttcaa aacgaagccc tcgatattcc agacgtccca gtttatagtc gaagtattca
     1441 tcaatagggc cggtatagac caccgtcttt gccactcctt catattcatg gcgaacctga
     1501 ttataatcga cgccagtccg cacttcgatg ccttcaagca gggcgttgat caatacatta
     1561 tagccgccga tgggaatgcc ctgataacgg tcattaaaat aattattatc aaacgtatag
     1621 cgtacaggca ggcgcttgat gatgaaagcc ggaagttccg tgcagctccg tccccactgt
     1681 ttttccgtgt accctttgat cagcttcgta tagatatccg taccgataag ggaaatagcc
     1741 tgttcttcca gattcttcgg ttccgtaatc cccgcttcct gacgctgctg ggcaatcttg
     1801 gcagccgcct gcttcggcgt gacgacaccc cacatcttag taaaggtatt catattgaaa
     1861 ggcaggttgt acaactcacc tttataattc gctaccggtc agttgacata attattgaat
     1921 tccacgaact ggttcacata atcccaaatc tctttattcg aagtatggaa aatatgagcc
     1981 ccatatttat gaatgcggat accttcgttt tcttcgcaat acccattgca gcccacataa
     2041 tcaggccgtt ccagaaccag acaagactta ccagccttcg tcatttcatg agcaaagaca
     2101 gccccaaata aaccgctacc aacaatcaga taatcgtaca ccgataaaac ctcctgtaaa
     2161 ataccattat gtatattgta ctacagtttt tctacttttt aacttttggt ataattataa
     2221 aattatttta atattaatcc atatgatatt tattttttct taattcaaaa gctatctttc
     2281 caccttctaa aatatcacat atatgttgtt gaattccttc agtattaaga ataccaggaa
     2341 ttaatcttcc attttcatct acttggggat gattattttc aattgaacca cttcgatatt
     2401 catatcgctt tgtaccattg gggatatcct tcccatcttg attagctaca atgacttctt
     2461 ctgcatctcc ccctaaaaca acatttgcat tatgagtaac aataattatt tgtcgctgcg
     2521 ttttttttgc acgaataaac ggaattaatt cattaaaaat agacctatta tctaaatcat
     2581 cttcaggctg atcgattaaa attggacatt tactttgatc taaattaata agc
//
LOCUS       DAWCYS010000245         2631 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1957_length_2631_cov_6.777950, whole genome shotgun sequence.
ACCESSION   DAWCYS010000245 DAWCYS010000000
VERSION     DAWCYS010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_2631_cov_6.777950"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          773 a          758 c          643 g          457 t
ORIGIN      
        1 gtatccacgc caacaactac caaagaaaac gtcgcgggcg ccgcccagga atcacagccg
       61 gagtctttgt atctcatgac agactcgcgc accggcagga agagcatttc cgccgcgaaa
      121 cccgaaccga cggaaccggc agaaacgaag acagaggaac cgcctgcaca ggatgagccg
      181 gaatcccagc ccacggaacc aaccgaaccg acagaaacga aaccagccga accggcggca
      241 accgaaccag ctgccgaaaa acagcccgaa ccgctcatcc acaccgaacc gtacacgctg
      301 gacgaactga ataccgctat cgcacagggg aacgtcaatg aaagccgtat cccccagcag
      361 tatcaactgc aatatgcaca gtaccagcag gaacaggcac gccgtcaaca gcagtaccag
      421 cagcagcagc aggccttgca gatgcaggcc cagcaacagc agttggaaca gcagaaaaga
      481 atgtttgccg atattgataa ggccgcgacc gaccaggcca tgaaggccct tggcattacg
      541 caggacgata ttgataccgc cgaatattcc gacgacgacg ccgtgaaaca gaaggtagcg
      601 catttcaata ctgccaaatc ctactacaag gagcagctta ttggcgctat caagcagcag
      661 cagatgcgga cgcaggccgc acagaacgaa cagcgggcca tttatcagag tattgttgat
      721 tttacgcagc agaaacaggc cgaagaacct cattttgccg acattaatca gttaatggac
      781 agttattatc agacaatgcc gtataaagac gccgctgtca tcggggatgc tatcaaagcc
      841 ctccagggcg gtaatatcaa tcccgcgcag tgcaaggtgc tagaaggcta ctacgacaaa
      901 tgccgcacgg catactatgc caaagcgaat gatctgacga agcagccgaa gaaagtaccg
      961 gtcccgaaag tcgaacagcc tggcacgggc gcgaaagcac cggctaaacc cattgatttt
     1021 acgcagatgc gaaatatgac agtccgcgaa cgccgcgcgt tcatcgcagg cctcagcggc
     1081 agataaagga gagatgaaaa catatggcat atgatgtaca gagaaacttg aataaatcgg
     1141 ccaaccagtc gtacacctac gacgctatcg gccatgcgga ggatatcagc cctatcctca
     1201 ccaacattac cccggaactt accttgttct attctaaatt cggcgactcc gaaccggcaa
     1261 aagccatgaa cttttcctgg atgacgaaag gcttgttccc gccgcaggat aacgcccacc
     1321 tcgaaatgga agactataag ttccagccgg gcggctccat cgaaggcttg tcgaacaacg
     1381 tccagttctt ccagaagacc ggcatggtat ccgacgcaca gaacaaagtc cagaaagcct
     1441 atcagaacga acacggctcc gaacttgcgg acctccgtta tgacgcttac acaggcctgg
     1501 cacaggatat tgaatacatg ctcgtcaact ccacgaataa agtcgacggt tcgaccacgg
     1561 tacagccccg ttccggcggc gtaccgttct tcatgcagca gaacctcatc gacgtcactg
     1621 tttccactac ggataacact atcacgtcgt ccacggaaac ccacctggct accggcgata
     1681 tctgctactt tgtcgctgac accatgccga cgggccttaa agacggcctc tactactacg
     1741 tacgcgtaga cagcaccaac agcaagaaac tgaccatctt tgatacgcag aaaggcgcta
     1801 tcgaaaacat cgaagacgac caggtaacat tgtcggccgc tggcacgaac gtcaagatcg
     1861 taacgaacaa cgtcctgtct ttaggcaaga agcgcacgta taccctcgac gacatcaaca
     1921 acgctatgga aatgacgtcc aaacgcggcg gtcatcctac gcaggcgtat atgtcgtcca
     1981 gtaagtaccg tgaattcatc aagctcgtcc tggctaccat gacggctacg cgccaaggca
     2041 atgaaaaggc cgatgccacg gaattcgcta cgtcctacca gggcgccttt ggcctcgtca
     2101 acgccaacgt ccatccgatt tacccggaca accgtatcga catcctcgac ctgtcttact
     2161 gggatatgaa gtacctcgac aaaccgcacg aagtaccgcc cgaaaaactc agtaaagacg
     2221 gcacgtacga aaaattcgtc atcgaagcca gcctgggcct caaaggcaca cagcctaaag
     2281 caagctgctc catcgttgac attaagcgat agtcaattcc ataaaagaag gggttcacgc
     2341 cccttctttt tatataaaag gtggtgactc tgagtgataa cgaaacagaa gatttaccag
     2401 aacggcgacg aaatctgctt gcggaacact gtcgacgtat caagtgccgt ggatgcggcc
     2461 cggcgcgtca atgagattga caacggcggc tgggctggcg acaagaacga acggattcag
     2521 ctcatgggct tcattccgcc ggaattctgg ggattcgacc cgtggctcat ctgcgccaag
     2581 agggcggaat tagaagggaa ccaggcgaaa taccagtact acatccagaa g
//
LOCUS       DAWCYS010000246         2626 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1962_length_2626_cov_6.428627, whole genome shotgun sequence.
ACCESSION   DAWCYS010000246 DAWCYS010000000
VERSION     DAWCYS010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1962_length_2626_cov_6.428627"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          675 a          719 c          780 g          452 t
ORIGIN      
        1 gccgatgacc cacgtactgt atacgccggt aatggccatg acgcccatgt tttccccata
       61 cgtcgtattc ggcgtcgaac cgaccaggcc ggacaggacg ttggacaagc cgtcggcaaa
      121 gagcgaccgg tgcaggcccg ggtccttgat gaggtcgcgg ccgacgatat cgctggtgac
      181 gaagagatgg cccaggtgtt cggccaggac gacgaacagg gccggcatga tcatcatgat
      241 agcgctgaga ttgaattccg gcttatagaa ggtcggaaaa gcgaaccagg gcatttcctg
      301 gacatgggtc caatcgacga cgcccatgaa gaacgacagg atatagccgg caatgacgcc
      361 gatgaggatg gggatgatgc cgatgaagcc ccggccgagg acggtggcca ggatggtgac
      421 gaccagggtg aacgtcgaaa tgatcatggc cgtttcattg gacatgccct ggacttcgcc
      481 catataaccg gacatattca tggccagcgg cgccagttcc aggccgatga tggctacgat
      541 ggagcccatg gcagccggcg ggaagaggac gtcgatccac ttggtgccga ccttgtcgac
      601 aatggccgcc aggatcatga aggacaggcc gaagacgatg aagcccccct gggcgtcgcc
      661 ataggacatg cccggtgtcg cgcagacagc caggaccggc gaaatgaagg cgaaactcga
      721 accgaggtag gccggcaggc gccatttaca aatggtcaga taaatgagcg taccgacgcc
      781 gttcatgaac agggccgtcg ccgggtcgac gtggagcagg aacgggacca ggaccgtcga
      841 gccgaacatg gcaaagagat gctgtaaact caggggaatc gtcggcaaga taggcagccg
      901 ttcttccaca tgtataattc ttcgttgttc cataaaatca aaatcctcct aaattcaatg
      961 ccccataaag ggcggacgca aagcctgccg ggcctgctgg gcactcaagt cctgcgcctt
     1021 ggccgcggcc atgtaattat catagctgac gcgcagcagg agcgacgcag aaatcaggtt
     1081 caaaataagg gatgacccgc cgtaactgat gaagggcagc gggacaccga cgacgggaaa
     1141 caggtccgta accatagcca tgttgaccag gccctggccg ccgagagaaa aggtaattcc
     1201 catggccagg aacatgccga accggtcctg acaggagcct gccgcctgga cgccaaaata
     1261 aatcagggcg aaaaacagga ataagaccag gacaccgccc aaaaagcccc attcctggca
     1321 aaggatagca taagcgaagt ccgtatgggc ttcgggcagg taactgaact tgctgatgcc
     1381 cgtccccatg ccctggcccc agacgccgcc ggaaccgatg gcgatgaggc tctgggttat
     1441 ctgatagccg atggtcttct tgtagtcctc agggtatatc caggcccgga tgcggtcttt
     1501 ctggtaatca tgaccaaaga aataaaaagc caggacgccg ataacgattg ccgcaagggt
     1561 ccacaggccg attttcttat acgaccagtg ccggtcatag agtgggatgc cgctgcaaaa
     1621 gagcatgaac gccgacggca caaagagcac gatagccgtg ccggcatcag gctgaatcaa
     1681 gataatagca gagaggaaga gagccggcaa gaggcagagc agaccatagc tcttgaagag
     1741 cttcttcttt gccaataacg acggcgccaa gtgctcgatg cgccattttt tagtgaatcg
     1801 ttcgacgaca gtctgctggt caccgtgaca ttcccaaaag aaagtcagcg ccgcagccgt
     1861 gcaaatgatg gctgccagct tagcaaattc cgacggctgg aaagacacgg ccccatagcc
     1921 cagccagcgc tgagcaccgt tgacggtgat gccgatggcc ttgacggcaa agagcagcac
     1981 ggcgctgatc aggaccagcg taccgcacca gcggcgcagc cacggatagc ccaggtgcca
     2041 gacgccggca gacgccagca ggcccatcac caggaagccc aattgtttca agagataacc
     2101 atagaccgta ctgccgccat cgaggacaaa ggtcgagctg aggatatttt ccatgccgat
     2161 gacgacgagg cacgaaaaga tacccaccag ccagaggacg ttagtcctcc ggcgccgggt
     2221 ccggacggtc agtctgggtt ttaacatgag caatcacttc cttgggacgc gtagcgacac
     2281 aacggaaaat gggctgtccc ttggcgctga acttcgcttc atattccgtc atggcttcat
     2341 tcaaaatcgg gctgttatgc agatcataag tcacttccga caagggccag ccacattcgc
     2401 ggaactcgtc gagggtgaaa tcgaagagac cggcattatc ggacttgaag taaatctggc
     2461 cattttcccg caggagccgt gcgtagcggt cgaggaaacc gcggtacgtc aggcgccgtt
     2521 tggcatggcg cttcttgggc cagggatcgc agaagttgat gaagaagcgg tccacttccc
     2581 ctggggcaaa actatcttcc aggcgtgcta cgtcaaagcg cagaag
//
LOCUS       DAWCYS010000247         2620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1971_length_2620_cov_4.100975, whole genome shotgun sequence.
ACCESSION   DAWCYS010000247 DAWCYS010000000
VERSION     DAWCYS010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_2620_cov_4.100975"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          508 a          735 c          790 g          587 t
ORIGIN      
        1 catgggctaa ggcgacggga atgaagccgg agcaggtaat gagggtcccc gtcagcatgg
       61 gcttggccgt cgccttgaag gcatagcagg ccgcatcgac gcggttcatg cccttctcca
      121 gctggaccgt catcatttcg acggcaatga tggcgtcgtc gacgaggagg cccagggcga
      181 tgatcaggga acctaaggaa accttatgca agtcgatgcc ggctacttcc ataacacaga
      241 agacgccggt caggaccagt gggatacaga aagcgacgac caggcccgtc cgcagtccca
      301 ggctgaggaa gctgacgacg aggacgatga tgatggcttc acgcagggtg ttggtaaagt
      361 cgctgatgga ctctttgacg acctgcggct ggtccgatac ctggtggatt tccatgccga
      421 ccggcaaatc ggatttgacg ctttcctgca acttcttcaa gttttcgccg agctggaggt
      481 tattgccgcc gctcttcatg gacaaggcga taccgacggc aggcttgcca ttatagaaca
      541 tcttcgggtc cgccggttcc gtatagcgcc gttcgaccgt ggcgatatcg cccaggcgga
      601 agacgcggcc attggcattg atgggcaggt tgcggatggc ttcgacgtct tcaaagacac
      661 cggacaggcg caggtagacg ttgtccgact gggtatcgac catgccggcc ggcgtcatct
      721 ggttctggcc tttgacggca ttggcgatgg tcgtcggcgc aatgcccagt tcggccagtt
      781 tggaattggc cacttcgatg tagacctttt ccgactgttc gccgacgagt tcgactttgc
      841 tgacatcgtc gacggacatc atcatgcggc gtatcttttc cgctttctga cgcaattctt
      901 catacgaata gccatcgccg gtgatagcat agatagaacc gtagacgtcg tcgaaacggt
      961 cgttgtaata aggcccatag acgccttcgg gcaaatcttt cttcatatct tccgtcaaat
     1021 tgcggacttc atgccaggtg ctgcggatgc tgtcgcgcgg cgccttgtcg tcgaggtaga
     1081 cgtagatgac cgactggccg ggccgggaat aactcttgat gtaatccagg ttcggcgtat
     1141 cctgcagttt cttttcgatt ttatccgtca cctgttcctg catctgttcc gccgtggcac
     1201 cgggccaggc gacggagacg accatagtcc gaaccgtata cgtcgggtct tccatgcggc
     1261 ccagtttcat gtaggaaaag ataccggcaa tggcgacgag gatgatgaag taccagacaa
     1321 ggacgcggtt gtgaatagag acttccgata aatttctcat tcatcttccc cctctacgcg
     1381 gactttctgt ccttcactga gcatatggac gccggccgtg acgacgacat caccgcggct
     1441 gaggcccgtg actttgaccg tattatcgcc aaaatcctgg accgtcacgt ctttcaggga
     1501 caaggtctta tccttgccga cgaaccagac ctggggcgtg tcgccggtct gatagatggc
     1561 ggccagcggc aggacgaagg tatcttcagc ggttgcctgt tcactgccgt tggcaaccgt
     1621 agccgtcatg ccgagctgca tgccgtccgg cggattgggc agggaaatat tgacctggta
     1681 cgtccgcgac gccgcatcgg ccataggagc cacttcgcgg acgacaccgg cggcctgctg
     1741 gttctggagg gcccagaagg tgacggtcac gttctggccg actggaaaat cggccaattt
     1801 attttccggt acattgacct ggacttccag gtcccctgaa tggaccaggg tgactacagt
     1861 ctggccggca gcgacgacct ggccgacttc tgccgatacg gctgagatga cgccgtcggc
     1921 atcggccgtg agctgcgtat aggataactg gttttcctgc gcctgctggg ctgccaaggc
     1981 ctggttatac tgggccgaag cctggtcgta agctgtctgg tactggtcca ggacggacgc
     2041 gctgatggca tccatatcgt ataattgctg ataacgggtt aaattggact gggccagctg
     2101 gagctgagca gcagcggcgt cgacctgggc ctgggtctga ttgacagcct gggccacgtc
     2161 tttcgggtcg accgtcatca ggacgtctcc ggcatggacc tgggagccga gctggacgtt
     2221 gcgggccgta atgcggccgc cggcctggaa ggacaaatta ctctggtatc ggcccttgac
     2281 cgtccccgaa tagggaccgg ccgtcgtgcc cgccgtttca ctgccgacct ggatggcctt
     2341 gaccagctgg ggctgttcgg cctgctgggt ctgctggccg cagcctgtca ggcccaaggc
     2401 cgtcaggacg gctatgacga ggactgtata tttccaatcg cgtttcatgg cttcatctcc
     2461 tttgtgggat taagggcagc aatatcggct tcagcggcat tgtcggccac ttgatgcatg
     2521 agctgcaacg acggcgccag cgtcacctca tcgacgccct ggactaggac gtcgacccat
     2581 ttcgttaaaa tctcttccat cgtatcctgc agctcccggg
//
LOCUS       DAWCYS010000248         2618 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1973_length_2618_cov_6.401873, whole genome shotgun sequence.
ACCESSION   DAWCYS010000248 DAWCYS010000000
VERSION     DAWCYS010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1973_length_2618_cov_6.401873"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          671 a          706 c          714 g          527 t
ORIGIN      
        1 gtatggatat caaccgcctg gtcgtcagta tgcagcagca gatccatgcg acgtcagtca
       61 tcgtcactca cgatatggac tcggccttct atgtcgctga ccgcctggct tatttacaag
      121 acgggcgctt ccggctcatt gccgataaga agacctttgc ccatacagat gatgaggaag
      181 tgcagcgctt tatccacggc gggcgtcttc cagaagaaag aggggatagc ctatgaagtg
      241 gagtggagaa gcaaaagtgg gattggtcac tatcgtcggc gtcctcttat ttacctttgt
      301 cgtcctgacc ctggctcatg ccgaaatctt tggtaagccc ggctttgtcg tccatacgga
      361 attcaaggat gccaatggcc tgcaaaaagg gaattccgtc cgctatgtcg gcgtccacgt
      421 cggtaaggtt gaaagtgtaa aaccgtcccg cgatggcgtc gatgtggccg tgaaactgga
      481 aaaagggacg gaaatccccc gcgattccaa agtgacgatt acgacggacg gcctgctcgg
      541 cgaaaaaatc gtggccatta caccggtgaa tgacaagaat catcttctcg ctgatggaga
      601 ttacatcgat ggcacacaga gcaagaacat ggatgacatg atggatagtg ccggcaagct
      661 catgggcagt gctgacgata tggtcaagaa tatcaatgcc atcatcggcg atgaaaagac
      721 gcaggccgct atgcgcggtt ccatccagca tatcgacgat attaccggca agacgagcca
      781 gatgatggat gccaatgccg gcaatatcca gcagattacg gctaatatgg cagccatgac
      841 agctcagatg aacgcgtcca tgcagaacct cgacggcgat ggcgcggctt cagctaacgt
      901 ccgggccacg gcggccaaca tgaagcagat caccgaccgc ttcgagatca ttgcccagtc
      961 gatggaaaag atgacgacgg atccgcagac ccaggccaat attcagacga cgctgaataa
     1021 tacggcccag attacaacaa aagtgaataa gatactcggc ggcgcatcgg acatcaaagt
     1081 ccagggcgaa gccggactct tatataatac gacgaagaat gagaccagcg gcactgtaaa
     1141 ttttaaggtg taccgtggcg atacgttcgg cctcgtcggg gcagagaaca tcggcaacgg
     1201 tacgaacctt aacttgcagt acggccgccg cggccgtctg ttcgacacgc gggccggact
     1261 gatcaacggc gacctcggtg ccggcttgga cttcttccgg gacgggcctt tccgcttgtc
     1321 cctggaaggc tatgatcccg atgactggcg cttccgtttg aaagcccagt accgcctgac
     1381 gccggatctc tatctcttca gccagttcac gcggcccatg agtcgcagtg acggtggcaa
     1441 ttattatggt attagttacg ctttttagca gattatagaa ataaacgggg gtacacatga
     1501 tgaacaacaa actctacaac aaattgctca tagccaagat gattacgacc ataagcgcag
     1561 tcgccgccgc ttcggcagcg gatacgacgg cttctgtccg tcctgtcgac ttagcgcagg
     1621 ccgatgcgat ccagtcccgc gccaaggcgg aacagcagga aatcaaggct gaaaaagctc
     1681 agcagaagaa agccgccaag gccgaagcca aagccaagaa ggctgccgcc gccgtccggc
     1741 ccgtagacat tacgccggaa ctgcgcgaag aactggctca gaaaatcgct gacgatgccg
     1801 ccaagagtgc tgaaaaagca gcggataagc gggcgaagaa ccaggaagtc gacccggtca
     1861 tcgtcgtcgg ccgcgtaccg accaatggca ctgtcgattt gaacctgccc aagaccgttc
     1921 agatggccct cgattacaac cgggatatca agaattccca gtacgccctg aagagggctg
     1981 aatacgccat taatcaagcc caggccggta agaagccgac tgtcgactat acctttggtg
     2041 ctcagcgtag tcgtgctact gacgttgcta cttatgcagg ttcttctgct ggttcaagtg
     2101 gtgcgtctcg ggcattacga gacattatgc gacaacttgg tatggatcct agtgcttatg
     2161 cgggaagtgg cagtgctaaa agtgccaata gtatcagtaa tgccttttct aatggcattt
     2221 ctgtaaatat tccactgtac acaggcggtc tcgttgaagg ccaaattgac gtggctaaat
     2281 tgggcaagac caacgcccag gaagaaatcc tgcgcgtcga acaggcgacg aaatattcgg
     2341 ctatccaggg ctattatggc ctcctggctt atcaggaact gcaaggcgtc taccatgaag
     2401 ccgtcgacaa cctccagggc cacttggata acgtccaggc tcagtacaac gtcggcacca
     2461 aggccaaggt cgacgtcctc tcgtctgatg tctccctggc caacgccaag acgacggcca
     2521 ttacggcaga taataacgtc gccgtcgccg aatcgaactt gagcagcatc ctcggcctgc
     2581 gttgggcaac gacgctcacg ttggccgccc ctccgcag
//
LOCUS       DAWCYS010000249         2616 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1975_length_2616_cov_5.151894, whole genome shotgun sequence.
ACCESSION   DAWCYS010000249 DAWCYS010000000
VERSION     DAWCYS010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1975_length_2616_cov_5.151894"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          584 a          673 c          843 g          516 t
ORIGIN      
        1 gggcaaagac agccaatcct tcaaggacta ctgcaatctg ctctacgatc aggctctgct
       61 cctggaaggc atcatgcccg acgaccccgt cgcctttgcc aataaagtag ccagcctgat
      121 ggcaaaataa aattccatac agaaaagccc ctcccggtgt gtgaccggaa gggacgttcc
      181 tccagcaaaa ggtcctgtcg ttagacgaca ggaccttttg ccgtaggggc cgtcccaagc
      241 ctttccagcc cgtcaggtgc caaccaacgg gaggcgcagg acggttgctg gaaaggacag
      301 ctgtggctgg gcggcccgct tgtgggaatt ccgaaagata gtgccgcagt tcgtaggcgg
      361 cagatcatcg attaacctct cagtcagctg cgctgacagc tctcctagca ggagagccgg
      421 gctgccatag gggccgcgag gcacgagcca caaactgcta accgctaacc cctaacccct
      481 agtggaatat gttgatgaac acggtgatga tcgatgcgtt gactgggtcg atgatgagcg
      541 agccgatgag ggggatgacg aagaaggccg tcggtgccgg gccgaagagt tcggtcatgg
      601 ctgtcatgtt ggcgatggcg ttcggcgtag cgccgaggcc gaagccgcag acgccgctgg
      661 tcatgacggc tgcttcgtag tccttgccca tggcgcggaa gacgatgaag taagcgaaga
      721 tggccatgaa cagggcctgg ctgaggagca tgacgaccag ggggatggcc aggtcggcga
      781 gctgccagag cttgaggctc atgaggacgc aggtcaaaaa gacggtcagg ctcaggttgc
      841 ccaggacttc gctttcgcgc tgatagattt tataggcgtg cgtgaagtcg gcgatgttgc
      901 ggatgatggc ggcgacgatc atggagccga tatagcccgg gaagaccagg ccgatgctgc
      961 tgaagaagcc gctgaccaga gtgcccaggc ccatggccag gaagaggtgg gccatggcgt
     1021 tcatgaaacg gtagttgtcg atggtctggc cgattttatt ttcaatctta atggcgtttt
     1081 cggcgcgttc tttattgccg gcgacggctt tgagcgtcga aacggtgccg gcattgaagg
     1141 acgggtcgaa gcgttccgat tcggccgagc tgacgaggtg gaatttgtgg accagttctt
     1201 cgccaatcgg gccgccgatg acgccgccgg cgacgaggcc gaacgtagct gctgcgacgg
     1261 caatggtcac gccgccgtcg aggccgatgc tttccaggag cgggccgaaa gccgctgccg
     1321 taccgtggcc gccgaccatg gggatggagc ccgttgccag gcccaggagc gggcgcaggt
     1381 taaaggccat agccaggccg gagccgatga tgttctggca gatgatcagg accgtcgtga
     1441 caatggccat tttcaggacc atgacgccgc cgcgcttgat gaggctgatg ctggccgtat
     1501 agccgacgct ggtgaagaag agcatcatgc agatgttctg catgatcgta tcctgttcgt
     1561 atttccagat gccctgcgta tataagatgc aattgatgat agcaaataag ataccgccga
     1621 cgacgggcgc cgggatacag tacttacgaa ggacgctgat cttgacgcgc aggaagacgc
     1681 caacgtagta gacgacgatg gccgcagcca gggtctgata aatattaaga tttaaagaca
     1741 cacggaaaac ctccttataa ttttaaaaat acaatacata tcataacaga ttttaggctg
     1801 taaaacaatt gtaaacatga gaaaatgttt tgatttttta agcatatatg ggatattatt
     1861 ttttaataga cagaaaaaat ggtgaatgat aataatcttg acggcctttt cgctggctct
     1921 ctttagaaaa acaaaagaac tggatgtcgc tggccttgag gtccagcttc agctgttcct
     1981 gtcccagggg agagaggccg ggagcgtcgg aacgcacttt ggtgaccacc gggagctggc
     2041 ccttggcccc tttcaggaaa gctgcaccgc ggctgttgag ggccaggagg cgggcatatt
     2101 gcggacctgc ctcataggag cagtccatga ggtcccgcga gggctgcaag agcgtatagg
     2161 cgcccatgcg gcagaggcgg ctgtaggcat agcgccgcgt cttgatgctc ctgagggcct
     2221 ggtcgtaact gccgctttcc atgaaagcgc ggtgccagcg gttttccaat ccttccgtaa
     2281 aggccggcag actgcgcagc tggtccggtg tcagcaggcg gttatggctg gcgacgaaat
     2341 cgccgtagcg gccgtaatca gtgaaggcgc cctgggccag ctggtcttcg gcacctgcca
     2401 ggctggccgc tgtgagataa ggccgacagg cggcggctgt cccgctgcag cgcatggcct
     2461 ggcgcagggc cgaggcgctg gcaaaggcgg caccgaggcc ggtatcgccg tgtcggccct
     2521 ggcggggcag gggaagaaag gtcaagggct gttcctgttt caacatggcc cgggcatatt
     2581 caaaggccag cagggcattg ggctgctgca gcagcc
//
LOCUS       DAWCYS010000250         2602 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1990_length_2602_cov_5.736160, whole genome shotgun sequence.
ACCESSION   DAWCYS010000250 DAWCYS010000000
VERSION     DAWCYS010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1990_length_2602_cov_5.736160"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          590 a          753 c          679 g          580 t
ORIGIN      
        1 gccttttaca tcgcctacag cgaaggcaag ccgcgctggt attacctggc ctacgccttt
       61 gccggcctgg ccgtcctgac caaagggcct atcggcttct tccagcccgg gctgatcatc
      121 ctgcttttcc tcgtatggcg ccgggatctc aaggccctgc tgcacatcaa gctcttttcc
      181 ggcctgatcc tgttcctggc catcaccctc atctggtatg gtccgatgta cgccatgcat
      241 ggcagcgact tcgtcagtca gttcatcggc gtccataacg tcctgcgggc taccgtttcc
      301 gaacatcctc agttcaatgt ctggtattat tacagcgcca tcttcctggc cggcttcatc
      361 ccctgggtct tcactctgcc cctggcctgg cgcgactatg gcctgacccg gaaaatcgcg
      421 gcggcctggc gggaaaaacg actgcgcctg cctgccttgt ccatgaagac ccagttcctc
      481 atcgtctggg cggctacgat tttcgtgacc tatcagctca tggcgacgaa atacatgacc
      541 tataccttcc cgtatatgat tcccctggcc atcggctttg cgtcgtattt gaagaaatat
      601 gaccgcctgg tcctcaacat ggccggcatc gtcctgacca tttacgtagc cgctacgtgt
      661 acggtcatcg tgcctcagtg ccagcaggct tcggcttatg atgccgccca ggtcgtcagc
      721 ggcctggccg ataatgaaac ggccgtcgtc acctatggcg gccgctaccc cgtatccctg
      781 acctattatt ccaatcatcc ggcttatcgc ctggaacagg ctgatgacat tgccaataaa
      841 ctgcctggcg gtatcagctg gaatgccaag aacgtcatgc ccttcatggc tatcgaagac
      901 ctgccgaacc atcctaaagt cctggccgtc gtccgcgacc gcgatgaagc ccacttcctc
      961 caggaagtac cggggaactg gaaatggcgc acccagaaag gcgtttgggc catataccaa
     1021 aaagaagccg atttgtagcc ctcctgaaat ggccttgtga ctgaattgta acggctcatg
     1081 agtatactat gagtaaccta aataaggaga gtgatactta tgaagaagtt caactatata
     1141 gtacgcatga tgctggtttt ggtcgttacc ttcatgacct tcagcggcgc cgccctgacg
     1201 gcccaggccg caggccccag agattatcca cagcgctggg aacagcacga tgaccgcgac
     1261 tatcgtcatg atgaccgcaa ctgggaaaaa gaacggaaag attgggaaaa gaaccataag
     1321 acatcgaccc agaaagatgt cgacaaagcc aaccgtaagg ccaacatcgc catcggcgtg
     1381 gccgccgtcg ccaccatcat cgccttgacg aagtgagtgg gaataatagt ggctagtggt
     1441 tcatggctct ccttatagga gagctgtcag cgaagctgac tgagaggttg atcttcgtta
     1501 tagtttgcag tattgagttt gtagtttgta gaaaattgct ttaaatttaa aagccttcac
     1561 tacaaactac aaactgcaaa cttaaaactt aaaaaggggc tgtcgcacat gcgacagccc
     1621 cttttttcga cgaacgatga acgacctacg actctaaccg ctaaccccta accgctaacc
     1681 cctaacccct agctacgaac cactagcccc tttcaattct cgtcccgtct gttataatag
     1741 ggagtgcaat tagattctgt ttataaagga agtgtttata tcatgaaatt aacggaccgc
     1801 ctggctgcgg ctgtatccct cattccacag ggggccgtca ttgccgacat cgggaccgac
     1861 cacgcctata tcccggtctc cgtctgtgag agcggccact gtccgtcggc cattgccgcc
     1921 gatatcgtcc cgggaccgct ggcagcggcc gtggcccacg tccgcggtgc cggcttgacc
     1981 gaccgcatcg actgccgcct gggtgacggc ctgacctgcg tcaaagcagg ggaagtcgat
     2041 ggcgctgtca tctgtggcat gggcggcccg ctgatggtgc ggatactcca gacttcctat
     2101 gcggtctggc agtctttgaa atttgtcgtc ttgcagcccc agagcgatgc cggtgccttg
     2161 cgccgctacc tgtatgagca tggctggcac atcgaagacg aacggctggt catcgacgac
     2221 ggccgccttt acgaactgat gcgggccgtg ccgggacagg ccggggccct gccggactgg
     2281 atctatgaca tcggccccgt gaactggcag cgccgcgacc cgctgctcag ccgcaagata
     2341 gacattctca tggaaacaaa agggcacatc ttggaaggcc tgaagaagag ccgcaaagat
     2401 gtgcacgaac ggatggaaaa aatagaaagc gaattgcgct tctggaggga attgaaatgg
     2461 caactacagt aggtgaaatc gtcaaagtat tgaaagcttg ggcgccgttg tcgctcaaag
     2521 aatcgtggga taatccgggc ctgctcatcg gcagccctga cgaaccggtc gataaactca
     2581 tggtcaccct ggacgtcatg at
//
LOCUS       DAWCYS010000251         2600 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1992_length_2600_cov_5.926523, whole genome shotgun sequence.
ACCESSION   DAWCYS010000251 DAWCYS010000000
VERSION     DAWCYS010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1992_length_2600_cov_5.926523"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          557 a          696 c          657 g          690 t
ORIGIN      
        1 tatatttttt tctattgata aaattcatga tatactggct tttgtttcta cgaaaggagt
       61 cttactatgg aatggattac gacgacggat attcacatcc tctttgggct gcaccgcctg
      121 gccgggccgc acatggatgc tttcatcact tacttcacga cggtgatcaa tacgctttgg
      181 ctctgggcca tcctagcggc cgccggtctt tggaaacggc ggagccgctg ggtcacgctt
      241 accatcatcg tcgccgtcat cctggccatc ctcgtcggcg atgtggccat caagcacttc
      301 gtcatgcgta tccggccgta tctggtcatc cccggtgcgc cgacgctgga tacgctgaag
      361 tacccgatca gctactcttt tccgtcaggc cattcgttct tcttctttgc cggggccacg
      421 gtcttattcc ggtcctgccg gcgcctgggc atagcggcct acggcctggc ctgtgtcgtc
      481 gccttttccc gtatgtacct gttcatgcat ttccccagcg acgtcctgac cggtgccgtc
      541 ctggggatta tcatcggaaa tattgcctgg cagatatgcg ggtatgccaa aaagaggctg
      601 tcgcattaag acagcctctt tttgagcttt aagttttcag tttgtagttt ttagtgaagg
      661 cttttaaatt taaaaccatc ttctacaaac tacaaacccg gtactacaaa ctataagaca
      721 aaagggacca cgcatgatgg cagagaaacc ttcgtgcgtg gtcttttttc aattaagctt
      781 ttgctttttt cggcactaaa tcaccgatcc ggtccaggat ataatggggc tggtaagaga
      841 actgctggat attttcccga ttagagacac cgctcatgac caagactgtt tccatacctg
      901 attctacccc accgatgatg tccgtatcca tccggtcgcc aatcatgacg gcttcttcgg
      961 aatggacgcc cagctttttc aagccagtgc gcatcattaa tggattcggc ttgccgatga
     1021 agtaagcttt cttccccgta gccagttcaa tcggtgcaat caaggcccgg caggccggga
     1081 taacgccccg gtcactagga ctggtcatat cgccattggt gccaataagc ttggcccctt
     1141 tgttgatcag ctcggttgcc ttgatgatca tttcataatt atagcctgtc gtttcgccga
     1201 cgactacgaa atccgtattg acatcatgga cactaatacc aacttcatac agagcattga
     1261 tcaggccagg ggctccgatg atgtaagccg aacagcctgg agcctgggtt ttcaggaaat
     1321 gagccgtcgc cagggcgctc gtataaaaat gactttcctc gacatccagt cccatagact
     1381 gaagcttttt gcgaagttcc agcggggtcc gttcactaga attggtaagg aacagaaact
     1441 gcttcttttc tttctggagc cagttcacaa aatcctttac ccccggaatc aaggtgctgc
     1501 cttggtagat aacaccgtcc atatcactga taaaaccttt tttagaacgt ataatgttca
     1561 aatccctcat gaattggttc ccccttcatt gatgagtgtt atttattcgc cacgaagccg
     1621 gcaatgacca tgcgctgaac ttcgttggtg ccttcgtaaa tctgcatgat cttggcatcg
     1681 cggacgagtt tttctaccgg atattcccgg ctgtaaccga agccgccaag gatctgaaca
     1741 gcttccaatg cgacctggac ggccatgtca ccggcaaagc atttggcaat ggcagcttcg
     1801 cgggtatagt tccgtttcgg tacggcatag taggtgttga ggaagttgat gattgccgca
     1861 cgggcgactt cggtcttcat gtccatgtca gccagtttaa aggcgatgac ttcgtgcttg
     1921 ataatcggtt tgccgaaggt gacgcgttgt ttggcgtatt tgaccgattc gtcaatagcc
     1981 cgctgggcaa tgccgacggc cagggatgcg accataggcc gggccaggtc cagggtctgc
     2041 atggcataga tgaagccctt cccttcctga ccgatgaggt ggtcagccgg gatatggacg
     2101 tcctggaaaa tgacttccgt cgtgttggac aggcggatgc ccatcttatc ttcttccttg
     2161 ccgacagaaa cgcctgggcg gtcccgttcg acgatgaaag ccgaaatgcc tttgacgccc
     2221 tttgtcttgt cggtaatggc aaagaccgtg tagaccgaag cgacgccgcc attggtgatg
     2281 aagcatttcg tgccgttcaa tacgtattca tcgccctgtt tgacagcgac tgtcgtcccg
     2341 gctgccgcat cggagccggc attggcttcg gtcaggcaga aagcgcagaa accgctgccc
     2401 ttttcaccgg tcaggtattc ggcatagagc ttcttctggg catccgtgcc gtgctggagt
     2461 accggtttca gacccaggcc ggaagcggca atagccgtag cgacaccggc gtcgactttt
     2521 gccagttctt catagacagc ggcaacggta taataatcct ggcccaggcc gccgtattct
     2581 tccgggattt ccaggccgca
//
LOCUS       DAWCYS010000252         2600 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_1991_length_2600_cov_9.201965, whole genome shotgun sequence.
ACCESSION   DAWCYS010000252 DAWCYS010000000
VERSION     DAWCYS010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_2600_cov_9.201965"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          508 a          695 c          783 g          614 t
ORIGIN      
        1 ctacgacaaa tcggttaaca gccgattgct ctaccaactg agctattgag gaataaatgg
       61 agcggaaaac gagactcgaa ctcgcgaccc ccaccttggc aaggtgatgc tctaccactg
      121 agctacttcc gcatgtgtgt tgtctgtttc agaagacttg attataatac caaatcagaa
      181 ggattctgtc aagggatttt ttgaaaaaaa ataaaaaagg cgctgtccca tgaagacagc
      241 gcctttttga gtggctagtg gttaggggtt agcggctggt tttaaattta aaggattttc
      301 cgcacgtggc ggacccgcta gctactgaca gctgactgct agcccctagc ccctaactgc
      361 taatccttaa tcactttcgc cttgaagacc agggctacgt cggcgtcgga acagcagaat
      421 tcggcttctc cttccggctg tcccggaagc tggccgccat agcgcaggat atcgacatag
      481 ggaaaggcac agtgcagggc catgggacag atttttcccc ggtccgtatc gtaatcaaag
      541 gtttccccga tgtgatggcc gcgatggcag cccatagtcc ccagtttccc cgtaatcgta
      601 agctggatag cgggacgttt tcctcgtgac atggtgttct cctttgtgga taagtaagca
      661 gaaattatcg cattaagcta tcggctttcg tgcggcaact ctgtttcaca ctgtaggggt
      721 cgccacgtgg gcgacccgtt gggcattggg tgacgctctc tccaagctgg aaaatcgctc
      781 atattccgga gcgggcttgc cacgtgcaag cccctacaag attgcaggtt acggaatcgc
      841 aaacagccgc ttggcgtttt ctgtggtcgc ccgggccact tcggcggcgt ctatgccccg
      901 cagggcggcg atttcttcgg cgacgtagtg gacataggcc ggttcattgc gctggccgcg
      961 gtgggcctgg ggcgtcaagt acgggcagtc cgtttcgatg agcatgcggt ctaacggcaa
     1021 atgggacgcg acgcgtttgg cccgtttgga attgggatag gtcaagggcc cgtcaaagcc
     1081 gaaatagaag cccatgcgca gcagttcgtc cgtcatttcc agactgcctg aatagcagtg
     1141 gaagacgccg cggatgccct tgccgtactg gcgcaggatg tccagcgtgt cgccgtgggc
     1201 ttcgcggtca tggatgatga tgggtacgtc gagttccaca gccagtttga ccatgtcgat
     1261 gaagacggcc tgctggacat cccgggccgg ttcaggccag tgataatcca ggccgacttc
     1321 gccgacggcg acgactttct cttccgactg tatccattgg cgcagcttgt cgacatcgcc
     1381 gtcctgggcc ttggccgctt cttcgggatg gatgccgacg gcggcataaa gaccgtcgtg
     1441 ttccctggcc atggcgatgc actgggctga cgtttccata tccgtcgccg ggcagatcat
     1501 gaggcctacg ccggctgcgg ccgcccggtc gaacacggcc ggatggtctt cagcgtaagc
     1561 accgtcattg acgtggcaat gggtatcgaa gagcatcagc ggacccggct tcccggtgcc
     1621 atgccgtcga cgaaagcgac ctgcaggtta tcgttgccat cggaagcagc caggagcatg
     1681 cctttcgatt caacaccgca gagcttgacc ggtttcaggt tggtgacgac gatgacgcag
     1741 tggccgatga ggtcttccgg tttatagtac agggaaatac cgctgacgat ctggcgcgtt
     1801 tccgtaccga ggtcgacttg cagtttcaac agtttcttgg ctttaggtac tttttcacag
     1861 gctacgattt tgccgacgcg gaggtcgatc ttgctgaaat cgtcgatagt gatttccggc
     1921 ttcgtcggtt cctgggcagc cgggcaggcc tgagcggctt tcttttcttc ttccgcttcg
     1981 gcgacttctt ccttcaaatc gtagcgcggg aagagggcat ggcctttgca gaccttcgta
     2041 tcagccggat aacggcccca ttcctgggca gccggcaggt tggcttcggc aaagtcacct
     2101 aagccgagct gtttccacat ttccgctgcg ctggccggga tgaaggggct gaccagggta
     2161 gcgatgatgc gctgggcttc gaggagattg tacaagaccg tctgcaggcg ctgggctttg
     2221 gcatcgtctt tggccaggac ccacggcgtc gtttcgtcga tgtatttatt gctgcggcgg
     2281 atgagggccc agacgtcgtt gatggcatcg ttgatcttca tgtcgtccat gtaggtttca
     2341 aaacgttttg ccgtttccac agcgcacgtt gccaaatcgt cgtcgaccgg ttctttgacc
     2401 gtcgggccgg cagcgacgcc gccttcgtat ttttcgacca tagccagggt acgctgcaag
     2461 aggttgccca agtcgttggc caaatcggag ttgatgcggt tgatgaacga cggcagggag
     2521 aagttgccgt cctggccgag gcggatttcc ttcaggaggt agtaacggag cgcatcggcg
     2581 ccgtagcggt cgatgagggg
//
LOCUS       DAWCYS010000253         2573 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2014_length_2573_cov_6.624305, whole genome shotgun sequence.
ACCESSION   DAWCYS010000253 DAWCYS010000000
VERSION     DAWCYS010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2014_length_2573_cov_6.624305"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          468 a          641 c          816 g          648 t
ORIGIN      
        1 gtcagcggga tgtagatgac atcgtcctgg tcctggccaa agccggagct gcccgtggat
       61 gccaggccgc cgatgatctt gtacggctgg ttgttgatgc ggatgttctt gccgaccggg
      121 ttggcttcgt cgaagaggtt ggacgcgacc gtcggtccga tgacggcgac gcggttgcgc
      181 gagttgaggt cgctctgtgt gatgaaggag cccgactgga cgacgaggga gcggatgtgc
      241 aggtattccg gcgtgacgcc gtagaccgat gtattccagt tctggttgcc gttgacgatc
      301 tgataggaac tgttgactgt cggcgatacg tagtcgatgt tctttatctt gttcttgatg
      361 gccgtcgcgt cgtcgtattt taatttctgg ttcgacccgg ctgccgagcg gacaccgccc
      421 tgattggtcg agccgggcat gacgatgagc atgttgctgc ccaggctggc gatggagttg
      481 gtgacgttct gcttgacgcc catgccgatg gaaatcatgg cgatgacggc accgacgccg
      541 atgatgatgc ccagcatggt caggatagag cgcagtttgt tggacaacag ggccgtgacg
      601 gcaatctgta cgctttcact aaataacatc catgacgacc ccctttccgg cgtctttggt
      661 aatcatgccg tccgagagca ggagctggcg gttggcacag gcggcgattt ccggttcgtg
      721 cgtgaccagg atgatcgtct tgtgctcttc gtggagtgcc tcgaagaggc gcatgatatc
      781 tttcgtcgat ttcgtgtcca agttgcctgt cggttcgtcg gccatgatga tatgcgggtt
      841 gttgaccaga gcccgggcga tggcgacgcg ctggcgctga ccgccggaca gttcgttggg
      901 cttgtggtgg gcccacgttt ccagaccgac ggatttcagc ttttccatgg cccgttctgc
      961 ccgttccttg cggccgacgc cggcgtagac caagggcagg gcgacgttgt ccagggcgga
     1021 aatgcgggat aagaggttga agttctggaa gacgaagccg atgtgctggt tgcggacgac
     1081 ggccagttca tcgtcgctga ggtgggcgac ttccttgccg ccgagcttgt acgagcccga
     1141 cgtgggccgg tccaggcagc ccaggatgtt catgagcgtc gatttcccgg agcccgacgg
     1201 ccccatgagg gcggcgaatt cgccttcttc tatgttcaag gtaatgccgg ccagggcggc
     1261 gaaatcttca ccgccgatgt ggtatatctt tttgatatct ttcaattcga tgacatgctt
     1321 catggcgatg cttcctttac atgggcggcg gcccctggcg ccggttgttg ttcgatgacg
     1381 aagatttgct ggaggccgta taggtgatgg aaaccttgtc gccctgggtc aggcctgaaa
     1441 ggatttccac gtattcatcg ctgtagatgc ccgtcgtgac cgggacctgt tcggtctggc
     1501 cgttggcatc gacgcggatg acataggaac cggaatcgtt ggtcttcaag gcggcgatgg
     1561 ggacgacgag ggcatcgtct ttttgcgatg tcgtaatgtc gacgcgggct gtcatgccta
     1621 gtttcaggac gtcgtccggg tcatcgacgt cgagggtgac gtaatagtag atgacgctgg
     1681 ccgaggagct tgaagaggag ctcgatgtcg aactcgtgcc attcgtgtcc cacgtgttgc
     1741 tcgtatcggt cttggcgatt ttcgagacgt gggccgtgaa agtccggtcc gtaaaggcgt
     1801 cgaccgtgaa ggtagcatcc tgaccgactt gaatcttgcc gatatccgtt tcatcgactt
     1861 tggccagaat ctgcttggtc gacgtgtcgg caatgcgcat gatgactgtc gggttgctgt
     1921 tgccctggac ggccatggtg ccgaccgcct tcggttcgcc gacgacgacg ccgtccatag
     1981 gtgctgtgat gaccgtttca gcgacatctg attcggcttc tgtcaaggtg ctgacggccg
     2041 tatcataatt gaacttggct gtatccaaag cctgtttcgt gccggcaccg agatcataaa
     2101 gctgctgggc ccggtcgtat tcgactttgg tattcgtgac tttatattga gcctggtcgc
     2161 gcttggcttc atagtccttg ccgtcgagga tagccacggt ctggccggct gtgacccggt
     2221 cattttcttt gaccaggacc tggcggatgc gggccgtgat tttcggtgag acttctacgg
     2281 aatcgacagg gctgatggtc cccgtcgccg aaaccgtcga gcgcaggtct ttgcggacga
     2341 cgtcggccgt ttcgatggcc ttggcctgct tcgccgtttg ttgggactgg taatatgtat
     2401 agccgccaaa ggcacaggca gccaggatga cagcggcaat gacgattttc aggcgcttgt
     2461 caattttcat ggtatcactc caaacgtata aataaaaaaa gattataaga ttattatagt
     2521 tattattgtt gttgttagct aagtatagca tgagcctaaa aaggaataaa gct
//
LOCUS       DAWCYS010000254         2570 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2016_length_2570_cov_5.481909, whole genome shotgun sequence.
ACCESSION   DAWCYS010000254 DAWCYS010000000
VERSION     DAWCYS010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_2570_cov_5.481909"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          646 a          740 c          714 g          470 t
ORIGIN      
        1 gagcaacctc tcagtcagct tcgctgacag ctctcctata aggagagcca cgagccacga
       61 attacgagcc actagccttt tcacaaaatt gtgttacaac ggccacagga aggcgaacca
      121 ggcgtggtat aatgaattgc aaaaaaattt caattcgagg tgacctcttc atgaaaggta
      181 aacatattcc cgctctggct gtgctctgct gtgccctcag cctgacggct cctgtctggg
      241 ccgcaccggc agtggatggc ttagatcaga cggccgtgac ggcgacggca gcggcgacag
      301 cggctccatc ttctatccgc gacgtgacgg ccttgagtac ggtctatgat gacggcgaaa
      361 gcgtatcggc tgtagccctt cattatccca agaccattga tgccagccgc ctgaaggcgt
      421 cggatttcac cgtcgccggc aagacgatcg acaaggtcta taccaatgat gcgccggaaa
      481 cggccctggc cggcaagaag ggcagttacg tcatcgtcag cctagtccat ccggctacgc
      541 ccttgccgac ggcagcttcc cagaagaagc ccaaagatga ccagggcgat aagggcaaag
      601 aaggctccaa cgccttgaac ggcggcgatg cgcccatgta ttccgaccgc aaggcaccgg
      661 acctgagcgt cgctgtcagc cagaccggga cgatctacgc gacggatggc acggcttatg
      721 gcccgacgac gacggccctg gctgccgaca agaccgtcga tgccgtcaag gacgccttta
      781 aaacttatat atataaggac ccggctacgg gctatgaaat gccgtataat atttacctgc
      841 ccaaagatta cgacgccgac aaggccaaga cttatcccat ggtcgtcttc atcgccgatg
      901 ccagcgccaa catcaacgac cccaaggccg tcctctatca gggcaacggg gccatcgtct
      961 gggcaacgcc ggaagaacag gccaagcatc cggccatcgt cctggcgccg cagtatacgg
     1021 aagacctcat ccgctctatc ggcatgatga ccaccgatac ccaccagtgg acccagggcc
     1081 tgaccctggt gacggacctc atcatggatg tatccaataa gtaccgcgtc gacaaggacc
     1141 gcatctacgg gacgggccag tcccagggcg gcatggccaa catcgccatc agcgacgcct
     1201 atccggaact ctttgccggc cagtacctcg tcgcctgcca gtgggatacg caggaaatgg
     1261 aaaaactcaa gggcaagaac ctctggatca ccgtctgcca gggcgacagc aaggcctttc
     1321 cgggcatgaa cgaagcgacg gccctgtggg aaaaggacgg cgctgtcgtc gcccgcagcc
     1381 gcatgtggga cagcacgggc ggtacgcctg tctttgaaca gatgatgcgg gtcatggaag
     1441 accagcaggc acccatcaac tatacggtct tcaaggacgg cagccacatg tatacctggt
     1501 cctttgccta caatatcgaa ggcatccggg actggctctt tgaacagacc aaggccgaag
     1561 atgtcctgaa gaaaaaggca ccggccggca tggatgccaa acagcacggc aaggacggcg
     1621 cccataaggg cagcccccag ggcaaagccc tgctcaacac gggtattgcc tacttgtacg
     1681 gccaggacgg tgcgacggac tacgacgtcg cccgcgaatg tttccagaag gcctggaacg
     1741 acggcaacat gaaggccggc cgctacctgg gcctcatggc cgctaacggc ttgggcgaaa
     1801 aacagagcga tgcccaggct gcccagtggt acgacaaggc ctagcagaaa ggcgatacga
     1861 ccagtatgta tcttttgggc aagtgctacg aacagggcaa gggcgtcaaa caggattacg
     1921 ccaaggccag gagcctctac gaaaaagcgg ctcagcgcat aaagcctccc ttactagggg
     1981 aggtggcccg aaagggccgg agaggttgaa tttcactgta ggggctcgca cgtggcgagc
     2041 ccgctgggta tatgtgagat acttgtgacc gatgggtgtg agcgggcgcc caacgtggcg
     2101 cccctacaga aaaaagagct gtcgcatgtg cggcagctct ttttttacaa ctaatttgac
     2161 tttttgactt atattctctt gacaaaacat attctccatg ctatcttata tatagtctta
     2221 gaagtcaaat agtcagacca ctaaaaatgg tcaaatgagc agaaggtgag tacgatggat
     2281 ttggatgcac gaaaacagaa aatactgcaa gccattattc aggattacat ctcctctgcc
     2341 gaacctgtcg ggtcgagaac gattgcccgc aagtatgact taggcgtcag cgccgcgacg
     2401 atccgcaacg aaatgttcga tttggaaata atgggctacc tggaacagcc tcacacgtcg
     2461 tccggccgca tcccgtcgct gaagggatac cgattctacg tcgactgcct gctcaagccg
     2521 acgaagctca cggacgcaga taagcaattc gacctcgcct ggttccagga
//
LOCUS       DAWCYS010000255         2564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2021_length_2564_cov_4.036269, whole genome shotgun sequence.
ACCESSION   DAWCYS010000255 DAWCYS010000000
VERSION     DAWCYS010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_2564_cov_4.036269"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          595 a          646 c          712 g          611 t
ORIGIN      
        1 atgacggaac aattcaaagg ggatgactcc gatcagtata cgcggtacga aacagatgaa
       61 cgcgtcggca acgacgttat actgtacagc aaacaacagc aacgcgctcg gtgcctgcaa
      121 ggaccggata aaggaaaaga taccgaacat gaagccgacg atcatgccga ccttgcggcc
      181 tgccgtcagg ctgccgatga tagtcgggat gtgaaggatc gtcgcgttca tgatgaccag
      241 cgggatgaaa ccatagcccg tgaggcctaa aaaaatcgta atcgccgata agaaaccggc
      301 aaccgttaac tgacgaatcg acaaaaaagt gcgtcttgcc ggcatgacgc cagcaacatt
      361 ctgtttttcc atgtttttcc tccgttctcg ctctttcgca agatacaagt cgcaatacga
      421 ttaaagacgc aaaaacacgg ccggtccgcg ccagtatccg cccgtttccg gtaccaaccc
      481 ggcgctttaa aagaacctgc cttatgtgtc acgcgtagta tatcatactt gaaggggtct
      541 tgccaatagc aattgggaat attgtgaatc tttacacaag cataatggag gataaaggcc
      601 ttcggccgca gttggcaaag aaacacggtc cagcaagaac gggctgtgga tttttctcca
      661 gcaggctcgc cctctatcat gaatcaaact tcaaccctga aaaagaaagg gactgtacgc
      721 gatagcacaa agcttcatgc gacagtccct tttggtgcga gtggagggac ttgaaccctc
      781 acggtgtgag gcgccagatc ctaagtctgg tgcgtctgcc attccgccac actcgcaagt
      841 acctttttat tctaccatgc ttccctccta ttggtcaaac aaaaaatatg gtatcatatt
      901 tctatatata tcataaagga ggattgttat gttattacaa agggaatcga aatttgaagg
      961 gtctgtactc ggatttatcg gctatggcct catcttagtc atccttacga tttgtacgtt
     1021 cggcatcgcc tatccttggg gcgaagtcat gtttcgccgc tggatctgcc gcaatacgat
     1081 catcgacggc cagcgtctca cttttgatgg aacaggtctg cagctcttcg gcagctacat
     1141 caaatggtgg ttcttcacta tcatcacgct gggcatctac ggcttctggc tgttcaacaa
     1201 gatgaccgcc tggcgggtaa aacatacgca ttttagggaa taataaaagg gctgtcgcat
     1261 cgcgacagcc cttttaagtt gttagtggct agcagttagt ggttagcgga tagttttaaa
     1321 tttaaaggtt ttctctgtga gaaaaggact acacatacga tgaggataac cagcgagtta
     1381 ctaaccacta accacgagcc accaatctca cacgacctga aacaggtaga acttcaggta
     1441 attcgtttcc gggacattcc agaggatggg atggtccggc gactgctggc gcccttcgat
     1501 ctggcggagg cggacgccgg cgtctttggc ggcgtcgtgg agcatccggc ggaacaggtc
     1561 gtcacgcatg aagtgggagc acgaacaggt cgcgaggtaa ccgccgcggg gcaggagttt
     1621 catggctttc aagttgattt ccttgtagcc ccggatggcg ttgtggacgg tatgacccga
     1681 tttggtgaag gccggcgggt cgaggatgat gaaatcatag tcatggcact tgtggtcggc
     1741 cagattggtc aagaggtcga agacgttggc ctgcttggcc gtgacgatgt cttccaggcc
     1801 gttgcggcgg atattttcgt tggtcatgtc gacggcttcc tgggaaatgt cgacggctgt
     1861 gacgctggcc gccccgccct tggccgcatt gagggcaaag gctccggtat gggtgaagca
     1921 gtccaggaca tgcttgccgg cagcgatttt ggcgacagcc aggcggttgt atttctggtc
     1981 caggaagact cccgtcttct ggccattttc cacatcgaca tcgtagagga tgccgttttc
     2041 gtcgatggtc gtcaagacgc tgcccggctg gggcaggaag tcggcctgat accagccttt
     2101 atactcttcc atgccgtcca gttcgcggac tttgacgtcg ttccgttcat aaatgcctcg
     2161 gatggtgacg cccatggcgg ccagttcgtc gacgagagcc ttgaagatga ccggtttgac
     2221 ctggtccatg ccatagcaca gggtctgggc gacgaggacg ttgttatagc ggtctacggt
     2281 caggcccggc atgtcgtcgg cgtctccgaa gatgaggcgg cagcaggcga agtctttatc
     2341 gcccatgacg gtcttgcggt agtccagggc atatttgacc cggcgctgcc agaaagcttc
     2401 gtcgaagcgg tcgttggcat tgcgcgacac gatgcgcacg cgtatcttag aaatgtcgtt
     2461 ggcaaagccc gtgccgagat agcggtcttt gtcgctgtag acgtcgacga tgtcccctgt
     2521 ctggtacgtg ccttcgacat gggtcacttc ttcgccatat accc
//
LOCUS       DAWCYS010000256         2564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2020_length_2564_cov_4.872858, whole genome shotgun sequence.
ACCESSION   DAWCYS010000256 DAWCYS010000000
VERSION     DAWCYS010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2020_length_2564_cov_4.872858"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          572 a          772 c          678 g          542 t
ORIGIN      
        1 acccctatct acatctatct attagtctgc agcgtcagtt gtgtattcta gtcagtatga
       61 tgacctcttt cccggcctcc tcgcgtgggg atgggaaggt ctctatgacg gcaaggccat
      121 ggccggcctg atctggctgc tggcctgtct cttggccttt ccctgcctgg tcgtcgtcgc
      181 catagcccgc tttttctgcc ggccccggcg ccgccggaga gggcgtcggc gtcaacggcg
      241 ctcgttgggc gtgacgcgcc ggtctttcct caaaggcctg gcggcgctga tccccgtggc
      301 ggctttcgcc accagcggag cgggcaatct cttgggcgaa cgcgatttgg atacgaccta
      361 ccatgtcttg aaatacaaga acctgcccga ttatctcgac ggctataaaa tcggccagct
      421 ctctgacctg cacatggggc tctttttcag tccccggcga ttgcaggaag ccctcgatgc
      481 cgtagcggca cagggcgtga accgcctgga aatcacaggc gacctcatcg atgaactggc
      541 cctgctgccc aaggcccagg ccatcctcaa agccaatgcc agccgcttcc ctgacggcat
      601 cgacttctgc tatggcaatc atgagtatta ccgcagtttc gatgcgatta cagccatgct
      661 cgatgacacg ccggtccggg tactgcgcaa ttccaccttc caggccagcc gcggctgcgg
      721 ccagggcctg aggggctgca atggccgcga cggccggtct ttttatatcg ccgccactga
      781 ttacagcttt gccaagggtg atgaagcctt tgccaaagaa cgggaacagt atgtccggga
      841 aacgctggct aaggttccgg aagatgcctt tgtcgtcatg ctggcccatc attcggcctt
      901 tatcgacgaa ggctttgccc atcacatccc cctgactttg tgcggccata cgcacggcgc
      961 ccagttcgcg cccattgcgc ccttggtcac ggccctgggc ttcaagtacc tccgcggcat
     1021 gttccagaaa ggggactgcc ggggctatgt caaccgcggc acaggccact ggctgccttt
     1081 ccgcgtcctc tgttcccgcg aggccagtgt ctttgaatta cgaaaacaag cgtaaaggat
     1141 gaatgcccat tatgatgatc aatacagctg tactccatat ttttgatttc aataccaacg
     1201 tctgcattgt atcccagaag gacctcgatt tcagcagcga tgtcgtctac gaatacgtca
     1261 gcaagcggct gaaccgcatc atcggcgatg ccgcccagaa gacgggcgtc ttctatgcga
     1321 ccagcgcctt tcagatgaaa ttgcaggccc tcgtcgacgg gaccctgact ttcgacgatt
     1381 tggcgtccca gagcgcccgc gagctctacc agctcgtgtc ccattgtgat gaaccggaat
     1441 cgacagacct cctggtcatc gacttccagg atgacgacga tgtccgcaac ctgggcatcc
     1501 tcatgctgga caacaagacg gcctataccc atcagatcct cgacgacgaa gggacggtct
     1561 acaacaagct catcaaacat tacgccatcc tgcccgggac ggcgcagaaa gccgaggcct
     1621 atgccctcat ccgcctgaac gatttttcta tccatttcgt cgacaagaag cggaaaatgg
     1681 acggtgaaga cgtctacctc ctgccggaca agctcctgca gtgcacgtcg gtcatttcca
     1741 gcaaggaagc cgtgaaggtc gtcagcaaga ttgccgaaaa agtagcggaa gaacacggcg
     1801 ccagcacggt cgaagcctta tccaaagcca agacctatct ggtggaaaat gccgaaacgg
     1861 ctgattcctt ctcgccgcag gacctgggca gcgacgtctt cggcgattcg ccggtcctgc
     1921 agcgggaatt tgaagaacaa gtcaaagaag cgaaactgcc tgaagccgta gccatcgaaa
     1981 aggaatatgc ccagaaggcc ggccgcagcc agaaaatcaa gaccgatacg ggcatcgaga
     2041 tcacgtttcc gtcggaatat atcgaaaatc ccgacttcat cccagtcatc accccccccg
     2101 atgggacctt gtccattgaa ttgaagaata tcgggaaaat cgtcaataaa taagggggat
     2161 tattatgtca cgcaagaatc ggcgcaaaaa agccaggaat actgcagccg ttccggaagg
     2221 gacagccgca acggcacagc cgaaaaagaa gaaaggaccc gctcacggtc tggaccggag
     2281 aacctgggtc ttgtacctcg tcggcttttc cgccctggcc tggtcctatt ttatcttatt
     2341 cacccgcaac tggtcggacg caggctttgc cttcaccgtc ctggttacgg ttctctggcc
     2401 ctggtctttg tataagaatt ggaagaaagg gccgaaaaag aggtaacgct tatggatatg
     2461 tcccattaca ataaagatta cgatacgtcc cacccggtcc ccgtcgtctc gccttatgaa
     2521 cctatgggct cccagcccga ggccatcgcc tctctggcag acgg
//
LOCUS       DAWCYS010000257         2560 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2025_length_2560_cov_5.412774, whole genome shotgun sequence.
ACCESSION   DAWCYS010000257 DAWCYS010000000
VERSION     DAWCYS010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2025_length_2560_cov_5.412774"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          536 a          665 c          650 g          709 t
ORIGIN      
        1 ccgtaaaatc ttcggcataa gcgaagacca ggcggccgcg taccgtaccg tagccggtac
       61 cgacgccgtc gccggggatt tccttttcct gcatgttgaa attcatgcag cgatgatgga
      121 tgaactgccc catttccgtg aagctgcctt catcaaataa cttagccagc cgttcgcggg
      181 ctgtcatctt gcctttttta tgttgcgaat caattcgctt ctgaccgccg cccatctcga
      241 ttttcgcgag cttttcatgg agctggtcaa tcttttcctg aactgtagcc attcttatac
      301 ctccagtatc aaagtatttt atcaggtcac ataacaataa agtatagcat gaaatgctct
      361 aaaatgctat atattaatca aagctgctca aaacagccgt tttccaatga ttgtattttt
      421 tcattatgtc catattgtac cacattgtat cacgttgtaa catttatttt tgacgatttt
      481 ttgacgagcc gtaaatggaa aacagcctgc cgtccgtgat ggatagcagg ctgttccttt
      541 atttatcgaa ctttaggacg actttcttag caaggtcatt tatcttggtt tgccgctggt
      601 cgatttcctg gcgtttccgg tccgggtcct ttttcggatc attgcggata gccttgattt
      661 ctttattgag tttcgatacc gaacttacag cggtgtcgac ttgtttcaat tccttcggct
      721 tattcgagga agacttctcg cccttgcgct gtttgccagc gtcggaacgt tcgtagtcgt
      781 cgttcatgtc cttctggatt tcatacatac ggttgacgta ttcgctgttc tggccgttct
      841 gacggatgaa cgagccaatg acaggcattt cataccaata cttagccggt tccgacggac
      901 ggctgttacc gcgtacatag ctaatcgggt cattgagcat attagctatg aaacggccgg
      961 cgctgccgaa ccatccacta atccagttat caatcgcaat cggcgacaac ttgatgctct
     1021 tggcaagcca ggtgtcacct aaagacttgg ctaattcgct tgtatagctg gtaaactgca
     1081 tttcggacgg agccttttgc aggctggcgg gtacgaggtt ccggcccgtc cagaacgaat
     1141 agttggtcat ccattcaatg agcgggcgga cgacagccgg atacaggctg ggcgtcatcg
     1201 cttcggcgta ctggcgcgcc cattctttca tcccgtgcgg gtccgtgccg gtcatctggt
     1261 tcaagacggc ttcgataccg gagccgaaca ggataccgac ttcctgcggc ttcgggatga
     1321 gtagatgcgt tccgcccggc agtgggatgg cccagttggt atacttcgtg ttttcgtcga
     1381 gttccttata ccaatcgtcg tcatggttga tagcccacaa tcccaacgac ggcaggataa
     1441 tccaaataac ggtattcaga atagcccgca cggggttatc cttgaacgtc cggataaact
     1501 tgtccgcacc ttgaatcgtt gcattgaaga acggcacgta cttattgact atctggccct
     1561 tcttgccatg gcgcgagaag tccagcgtga tttcacgtgc cagctggcct gcctggtcga
     1621 tggacaagcc ctggttgcgg gcgcgtctga attcgccggc gcgggtgccg tcttcgatga
     1681 gattgccgaa cttttcgtac ttgtgccatg catagctgat gagctggcgg acctgcttca
     1741 cgataaccgg gtacgagtcg tacttatggc ccagtaattc cttggtgagg tcacggctgc
     1801 tgtccgcgtc cgcccgcata tacgtcgaca tgctggcccc gctggcatga tattcggcgg
     1861 agaattcctt atcaaagtgc agtttccagg caccacgtac attatcgacg aatggcagat
     1921 agaacccggt cttggagttt accgtggccg acatggtatc acgcaataag ttacggacga
     1981 tgaaggacgg gctgatagtg gccccagtcc gcaaggtctg cgcgatactg cttgccatct
     2041 taatcgtgaa ccggttcgtt tgcgcgtcgt tattcgtgag tgcgtcgtag atttccggcg
     2101 tcgtgcggta tacgacttgt tccccgttct gccataccgt gaaggcaaag gccgccgggt
     2161 cggtgtgttt caatgtcggg tcgcggacga cgacgtcacc cagcccggca aaatcctggc
     2221 ccagcttgac gaatgtcttg gcgacgtcgt tccgttcgca tttgctaatc atagataccg
     2281 ccatctgcga caacgaggtt atcgggtcta ggataggccg cttaccgccg ccttcgatac
     2341 gcttgatacc gttgctgacg ttggcatagc cgccgcctta attgaatacg ccgatagtac
     2401 ctatcatctc atccatatct gtgatactgt ccgacatgtc gcgatacatc gggcaataat
     2461 gcttatattt gcggagtttc tcggcgatgt ccttggacat aaggccctgc tgttggagaa
     2521 tgatgacgat attcgtactg atatcccagt acatctgcgc
//
LOCUS       DAWCYS010000258         2557 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2028_length_2557_cov_4.020384, whole genome shotgun sequence.
ACCESSION   DAWCYS010000258 DAWCYS010000000
VERSION     DAWCYS010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2028_length_2557_cov_4.020384"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          532 a          680 c          726 g          619 t
ORIGIN      
        1 tcgtaggcga cgcggtcgtc gtccctcccc tggtagaacc ggaattcatg cacgtcctct
       61 acgtcatctg gaaacggggc cactacctct cccatgccgc ccagttgtgg ctcgacttcg
      121 tccgcaataa ggcccgcgtc atttccaact tataaggctt atggacagac acaagttccc
      181 tcctgacctg acggggatcc gccaaaagga gcagcacggc cgggcggccc tctcccgcct
      241 gcgccgccgc ttctgccagg cctggcacgg tctgccaaag gcccagaaat gcgtcgtcat
      301 cgtcgccctg ctggaattct tctggatcgc agccttgcag ttccaggtct atgaactggc
      361 ggacaccgta tccacccagc gcgttcgcat ggcacagctg gaaaagcagg ccaagaaaca
      421 aaataatatg ttatattccg cctcgcagac actggacgat atcgaaaaga aatggcaccg
      481 cctcaatttc cgcgtcctcg aaaacacctg gcgcatcgaa gaaccggaaa aagacccgga
      541 cgctttatag tttggagttt gcagtttgta gccggtggtt ttaatttttt cagccattca
      601 ctgcaaacta aaaacttaaa actcaaaaaa taggctgtca cattaatgca tgttttgctt
      661 aatgtgacag cctatttttt acacttttct ttaattttcg atgattttca ggaaggcttc
      721 cggttccggc agggaccgca gtatcttcca gggcgcgcac tgggccagtt cctcatagga
      781 atagcggcct gtagccacgc tgatcgtgcg ggcgccgatg gctttgccgc actggatgtc
      841 gtaaggcgtg tcgccgatga cataagccat ctcgatggca tcgccccagg cttccttggc
      901 gatggccagg gcattgcggg ccagatcatc gcgatagtaa taattgaagg cgaacccaga
      961 atgagagaaa tcgaagtact gggacaggcc gaagacgtcc atcttcatgc gggcgccgat
     1021 ggcgctgttg ccggtcagga ggagctgcct gtaatccttt cgctgttgga agaaagccag
     1081 gttctcatct acctatggca ggacgacgcc atcttcggcc ttgagctaca gccaattcag
     1141 gagattcttt tcatagcccc ggcaaaaggc atggacttca tcgtctgtcg gcatgatgcc
     1201 tgtggcttta tacaagagct gctggcagat gaaattatcc gtccggccgc cggcggcgat
     1261 cttcggcacg ggcacgtcgg cgcccttgag gtcgtggaag acttcttcaa tggcgtacag
     1321 accggcccgg cccgtattca ataaggttcc gtcaatgtcc cagtatagta atttcacagt
     1381 attcactcca tcttcgggca cgcactttgg tcattactgt ttggatacga tgtccttcgt
     1441 atcgcgggcg atcatgattt cttcgttcgt cgggatgacg tacatcttga ccgtcgaatc
     1501 atccgtgctg atgagggcat ctttgccgcg gacgttgttg cgctgcgggt cgagtttcgc
     1561 accgaggtat tccaggcctt cagcgatggc tgcacggtct tcgatgccgt tttcgccgac
     1621 gccagccgta aaggtgatga cgtctacgcc gcccatggca gctgcgaaag cgccgatttc
     1681 cttgcggacc tgatagtgga acatgtcgag ggcgagctga gcgcgttcgt tgccttcagc
     1741 agcagcctgg ccgaggtcgc ggaagtcgct ggaaacgccg gaaatgccga ggacgccgga
     1801 tttcttattc atgagcgtat ccatttcttt cgtcgaaagg ttgtggttgt ccatgacgtt
     1861 gaggacgata gccgggtcga tatcgccgca gcgggtgccc atgaggacgc cggcgagcgg
     1921 tgtgaagccc atggtcgtgt cgacgcattt gccatatttg atggcagcca gggaagaacc
     1981 gttgccgagg tggcagttga tgatgcgcag tttttcaacg ggtacgccca tgactttagc
     2041 gacttcgcca gctacatagc gatggctcgt gccgtggaag ccataacggc ggatgtggtc
     2101 ttctttgtag agttcatacg ggaggccata catgaaggct ttcgccggca tggtctgatg
     2161 gaaagccgta tcgaagacgc cgacctgagg aacgccgggc atgatggctt tgcaggcgtt
     2221 gatgccgatg atgttaggcg gattgtggag cggagccata gccgagcatt tttccaaggc
     2281 tttcatgact tcatccgtaa tgagcgtgga agcagcgaat tcttcgccgc catggacgac
     2341 gcggtggccg acagcgtcga tggcatccat ggatttgatg acgccgcatt cagcgtcggt
     2401 gaggatgtcg aggacgagtt tgacagcgac ttggtgatcc gggatggact gtttgatttc
     2461 tttcttctgg ccattccatt tgtgggtcag ctgcgaatcg ggaaggccga ttttttcgcc
     2521 caaacctttt gccattactt cttcattgtc cattgtg
//
LOCUS       DAWCYS010000259         2551 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2032_length_2551_cov_5.763221, whole genome shotgun sequence.
ACCESSION   DAWCYS010000259 DAWCYS010000000
VERSION     DAWCYS010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_2551_cov_5.763221"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          647 a          753 c          619 g          532 t
ORIGIN      
        1 ccctatggac gactaccagc cgaaactgcg ggaaacggcc agcctggtca tcgccggcgg
       61 caatgacatc atcgactacg ctccggccaa atggcatctg accaatgccg acatcatcca
      121 catcgacacc atgccggccg atatcaacaa gaactatcag cccgaagtcg aagtcgtcgg
      181 caacatcccc aactcgctgg aacgcatcat ggccaaagcc gcccgcctgg ctgaaccgaa
      241 acagttcctg gatatccgtc agaagatggt cgacgaacac gaaagctacg cccacgatat
      301 ggccttcccg gtcaagccgc agaaagccct catcgattgc cgccgcgtca tgggcaaaga
      361 cgacatcgtc atttccgacg tcggtgccaa taaagtctgg atcgcccgcc attacaactg
      421 ctacgaaccg aatacgtgcc tcatctccaa cggcttcgcc accatgggca tcgccgttcc
      481 cggcgccatc tcggccaagc tcgtccatcc ggacaagaaa gtcctggcca tcaccggcga
      541 cgccggtttc ctgatgaacg tccaggaatt tgaaacggcc taccgcctgg gcacgaactt
      601 cgtcatcctc atcttcagcg actgcagcta tggcctgatc aaatggaaac aggacatgca
      661 gtacggccat caccagtacg tcgacttcac caacccggac ttcgtgaaac tggccgaaag
      721 cttccacgcc atcggctacc gcatcaccaa gaccgaagac ctcctgccga ccctgaaaaa
      781 agccttccag cagaacaaac cagtcatcat cgactgcccc atcgactacg atgaaaacat
      841 gaaactcaca gaacatctgg aagaactgat gaaaacgtta taagaaaaag gcgctgtccc
      901 ataaagacag cgccttttta gtggctagtg gcccgtaggg gccgtcctat caaggcggcc
      961 cgcaagctag tggctagggg ttagtagtta gtggctaggg gctcgcacgt ggcgagcccg
     1021 cccagagttc ggcgacaatg ttcccggcct tcgcagggat ccggcccgcc tggatatcgg
     1081 gcgccaaact acaaactccg tactataaac tatttcttag gcatcttttc tgcgtttttc
     1141 aacatatccg tgacggcgct gatgatgtcg tcatcgcctt ctttggcgat gttgcccagg
     1201 atggcggcga cttccattgt atcgccgtgc tgccaggcca ggcagtggat ggtttccgtg
     1261 aagggattgc cgttgatgac cagggatttg gcgatggtcc attcataaaa gagatggcca
     1321 ttggtctttt tcgccgtcac cggcgagacg acgtggaagc cctctggaag gcgtcccggc
     1381 atattctggt tcagccggtt catgaggatg cgcatatccg gccggctgcc gatatcgcgg
     1441 agataagagt tttcccggtt ctccccatag acggccgcct tgacgtccca tatggtcgcc
     1501 gtataatgca gtagataggc atactggaaa tcggcatcgt ccttcttgac gacctgatac
     1561 gtttccggct ggataggaaa gattcgttca aacagggcga tgacatcgtt gccttcatca
     1621 gcagccgtca gcatgggctg ccgccctgac tgcatgtaga tatcatcggg gaccgtgatg
     1681 acgccccagt tggggaactt cgacagcgtc cctttatcct tggccagggc agccgtcccc
     1741 atcgccaaga cggcggctgc caccagcacg attgctttaa ttttcatggt ttcactcctc
     1801 gtttttctta cacaatgaca cgtccattgt atctcatgac gctttgtctg tcaaaaaaga
     1861 ggaaagtatg atatgatata gttatgtaaa tcgcctcaat ctatacggtt caggcatatt
     1921 cccatttatt taggaggtta taccactatg gaattcaaca tcaaagccaa ctcgacagca
     1981 gaagtttctg aaaaatgcac ggaaaacaat acggccatca aattgggcag cggcaaatcc
     2041 ccggtctacg caacgccggc cctggtagct ctcatggaaa acgcagccat caacgcctgc
     2101 gaccctcagc tcccggaagg ctataacacc gtcggcatcg ccatgaacgt caaacacgtc
     2161 gccgctacgc ctatcggcct gaccgtcacg gccaaagcca ccctgatcga acaggaccgc
     2221 aaaaaactga ccttcaaaat cgaagcccac gacgaagccg gcctcgtagg cgaagccacc
     2281 cacgaccgct tcatcatcga atcggcaccg ttcctcgcca aagccgaagc caaaaaagct
     2341 gcagcaaagt agtggctagt aggggccgtc ctgtcaaggc ggcctgcaag ctcgtggctc
     2401 gtaaaaaaag gcgctgtcgc atgaagacag cgcctttttt gagttttaag tttgtagttt
     2461 ttagtttgca gcaaatggta aaaaatttaa aaccatcttc tacaaactac aaactccata
     2521 ctacaaacta tgaagcaaag gaacctcgca t
//
LOCUS       DAWCYS010000260         2548 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2035_length_2548_cov_5.972323, whole genome shotgun sequence.
ACCESSION   DAWCYS010000260 DAWCYS010000000
VERSION     DAWCYS010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_2548_cov_5.972323"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          652 c          773 g          564 t
ORIGIN      
        1 gagtaagcac ggagagagga ggtcgatgat ggaagggaag gtttggcaga atttgatcga
       61 tgccggttgt tcggcggctt ttatcgaaca gtatgaggcc ctgcccgagg aagagcagtt
      121 gtcgtgcctg cagcgccatc gccggtactt gctcgatgct attcatgaca aacagcttca
      181 gctggaccgg ctggattatt tcttatatgt gttacgaaaa agaggagacg aaagaaaatg
      241 actttagaag aaatcaagca gacgagcccc tttgccatgg gcggagaaaa cgtcgactat
      301 gcccagtatt ttgatggcac cagttatttg aacatgttgt ccctaaaaca ggtcgtcgtc
      361 ggcaatgtca cttttgaacc ggcctgccgc aatcattggc acatccacca tgccagccag
      421 ggcggcggtc agatgctgtt agtcacggcc ggccgtggct ggtatcaaga atgggggaaa
      481 ccggcccagg ccctcctcgc cggtgatgtc gtccacatcc cggccaacgt caagcattgg
      541 cacggtgctg ccaaggacag tgccttccag catctggcca ttgaagtcga cgggactgat
      601 acgagtacgg aatggtgtga agccgtagcg gaggccgatt atgatgccct tccgtaagca
      661 cttcttagcg gccgggctca tggtcctggc gggaatcgga tttctcccct tttacagcgg
      721 ctggcccaag gcctgggcgg ccttcggcta tgccaaggaa gtctacgggg agtaaatgtc
      781 tctatttgaa ataatcagga ggtaaaaagc agatgatgaa gaaagtttta atgcttgccc
      841 tgagcagtct catggtcctg ggcctggctg cctgtggcaa tacgaacggc gccgatgcgt
      901 cttccagcgc ctcaaaaaag acggatgcgg cggcccaggc cccggccaag ggcaatggca
      961 agacgctggt cgtctattat tcggctggcg gtcataccaa gaatgtagcc aattacattg
     1021 ccaaagcgac caacggcgac gttatggaac tgaaaccggc ccaggactat acggaagcgg
     1081 atctggacta caataaccgg gacagccgcg tctatcgcga acacgatgat gccagcctgc
     1141 gcgacatccc tctggtcaag gataaggcag acaactggga aggctacgat acggtcttca
     1201 tcggatatcc tatctggtgg gggatcgctg cctggccggt agatactttc gtcaaacaca
     1261 atgacttcag tggcaagacg gtcatcccct ttgccacgtc ggccagctcc ggcctcggcg
     1321 acagcggcag acagctgcag cagatggctg gtacgggcaa ctggctggaa ggtaaacgct
     1381 tcccgtccaa tgtgtcggaa agtacggtcc agcagtgggt ccagagcctc aatctatagg
     1441 ttccagcgca tccttttcct gctttaaaag ctggatgaat tgctgcgtcc ctttggggaa
     1501 cagctgaaaa cgcttccagg cgatgagcag cctgtccgaa aggggcgggt ccaggaggcg
     1561 gaaacagagg gtatcatcgt cttccgggac acggatgatc ttttccagca tgatgcagga
     1621 ccccagccct tcgcggacca tgagggacgc attataggcc aggttgtacg tggcgctgat
     1681 atgcaggtca gcgaagggct ttttcagcca gactcgcagc ctgtctgcct gattgtcatc
     1741 ctgcaggacc tgattggata cgatgagttt ttcatcccac aggtcctgcg gggtgatttt
     1801 ttctttttgc gagagcgggt tatcgcggcg caggaggagg ccccagcggt cgacagttgg
     1861 gcaggggagg acttcgtatt tatccatatc gacgtggcca tagacgacag ccatgtcgat
     1921 gaggctcttg tcgaggagtt ccatcatctg ctgaccatcg ccgctggtga tatggaaatg
     1981 caggtgggga tacctggcct ggatgtcatg ggcggcgtgc atgaggaagc ggacgctgtc
     2041 cgattcggcc gagccgatgc ggatggtccc gttcagttcg gtgtcgtcgc tgcgcagttc
     2101 ctcttccgta tgactggcca gggagaggat ttcttcagcc cgctttttca ggaataagcc
     2161 ttcttcggtg agcgtaatgc gccgggggcc gcgaatcatc agttcttttc ccagcatctc
     2221 ttccatgtcg cgcagctgcc gcgacagcgt cggctgggat aggtgcagtg cctcggcagc
     2281 atgggtgata ctttcttcgc gggcgacggt gaggaaataa ttgagtaccc gtaaatctaa
     2341 catccgaatc agtcctttcc ttgccaatgg atttcttctt tcaggccctt caaaaaggct
     2401 tcgcacgccg gtgacaggag ttggtatttt ttccaggcca cgagacacga gaaggtgacg
     2461 gctggtttca gggggacgaa ggccaggtcc ggcgtatgga gctggaagag ctcgtcgatg
     2521 ccgagggcac tggtaatgcc cgtccgga
//
LOCUS       DAWCYS010000261         2548 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2036_length_2548_cov_5.432010, whole genome shotgun sequence.
ACCESSION   DAWCYS010000261 DAWCYS010000000
VERSION     DAWCYS010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2036_length_2548_cov_5.432010"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          693 a          711 c          671 g          473 t
ORIGIN      
        1 cccgcccgac gcgcatcctg accggctacc acccgccaat gcccccgcga ttccctgcct
       61 gcgcggacat gcacgcagta tgagaaagaa aagattgttt tacgtatcgc acccgtacgg
      121 cggcaagcct gaaaatgaaa aggccgtaca agccgttata gaagaactga aagaaaagca
      181 catacccatc agcaccgact ttccgacaat gtactacagc aacgtggaat tcgtatcgcc
      241 gattcagctc ctggggccgc tctacgatgc tatgccctat ctcgaaggac tgaacctgtg
      301 ccttagcctc ttgaaacgct gcgacaccat catcatgtgc ggtgattggc agaactcgcg
      361 cggctgcatg gccgaataca gctatgccct ggctaagcat atccctatta ttagtcagga
      421 ggatttggaa tggtgaacgt cttagatcag tacgtatcgg accgggtatc cctttacaac
      481 ggcgacagcg tagaggtcct caagggcctg cctgataact gtatccatta tgccatcttc
      541 tcgccgcctt tctcgtcgct gtatacctac tccaacagcg accgcgacat gggcaacagc
      601 gcgagcgatg aacagtttaa tacgcatttc aagttcctga tccgcgaatt ggcccgcgtc
      661 atcatgcctg ggcggcttgt atcggtgcac tgcatggaca ttccgaaaat gaaaagccgg
      721 gccctgggct ttgaattaaa agaatcgtac tacaaacaaa tggtactgaa cgtcaaaaag
      781 gaggaaaacc ttgatgaaaa ttaatcttga atttaacgga accgctgatg aaatcatgaa
      841 agaattccag gcactcggct caatcttgag ctcagcagca ttgccctcaa atgtgaagtc
      901 aatgaatccg gaagcactga gtccagattt gggctcagtg gaacagcatc caaaaatgga
      961 tgcagtagaa aacacaactt ccgcgccgaa aaaggccaca aaagcgccgg aaaaggccca
     1021 tcccacgctg gaagctgcac cttctgcgcc gacaccggaa ccggtgaaat ccgctgatcc
     1081 ggaacctgta aagcctgccg cgcctgaacc gcctgcggct cctgtcgtgc cgacggcacc
     1141 ggctaagaag tacacgctgg acgaactgct ggccgcgacg gcgccgctga tggatgcggg
     1201 caaaatcgct gagctccaag cgctgatgca gaaattcggt gtaccgtcga tgatggacat
     1261 cccggaagaa aaatatgggg aactcgcaac tgccctgcgt gaattggggg cggaattatg
     1321 agccgacaac atgcactctt aagcgcctcg gcgtccgcgc gatggctgca atgcacggcg
     1381 gcgccgcaga tggaagcgaa attcccggat acgacgtcag aatttgccag ggaaggcacg
     1441 ctggcccaca gcatcgcaga actcaaactg cgggcctatg ccgtcgaacc gatggccaag
     1501 tcgacgtata caaggcacct gaacaagttc aagaaagatg agctgtatca gccggaaatg
     1561 gatacccaca cggaaaccta tctcgactac atcaagggca tcctgctgtc gtacaagaca
     1621 aagccctata cggttgtaga aaagcgcgtc gactttagcc aatatgtgcc gcggggattc
     1681 ggtacagccg actgcctgat catggcgccg aatgaactcc acatcgtgga tttcaaatac
     1741 gggaaaggcg tacccgtcga cgccaacaac aactcacaga tgcgcctgta cgcgctgggc
     1801 gcgctgaacg cgtaccagct actgtaccaa ttcaagacgg tacacatgca catcgtacag
     1861 ccgcggatcc agaactttag tcaggaaacg ctcggcgtcg atatcctgcg gcaatgggca
     1921 gaagacgtcg tcaagccgaa agcccaggag gccctgagcg cggacggcgg gaaattccat
     1981 cccggtgaat gctgccgatt ctgccgggca aaggcccaat gcaaagcccg tgccgaatac
     2041 tacgcagcta tggctgacac ggcgcacgaa aagcgcgata tgacgatgat caacatgacc
     2101 gagctcggcc aatacctgac catcgccaaa cagctcaagg cctgggcaga cgacttgcag
     2161 gaatacggcc tttcctgcgc gctgaaaggc ctcaacgtcc ccggctggaa atgcgtagaa
     2221 ggccgcggca gccgggcctt taccgacacg gatgcagcat tcaagacgct catcgataat
     2281 ggtatcgatg aatccgtcct gtatgaacgc gtgccgctga ccctggctaa aacggaaaag
     2341 gccatcggca agaatctctt taccgaactc gtcggcgatt acgtcgaaaa gaagccgggc
     2401 aagccgacgc ttgcaccgga atctgacaag cggccgccga tggacctgac gccgaaagcg
     2461 gctgatgtat tcaaaaagat ttatgattaa ttggaggaat gaaaatggaa aataccagca
     2521 ttgtagtaga aaacgtacgt ctcagcta
//
LOCUS       DAWCYS010000262         2545 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2042_length_2545_cov_4.701205, whole genome shotgun sequence.
ACCESSION   DAWCYS010000262 DAWCYS010000000
VERSION     DAWCYS010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2042_length_2545_cov_4.701205"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          486 a          763 c          695 g          601 t
ORIGIN      
        1 ctggacgacc ttccccttga ggattacgtc ggtctggtcc tcgccgagga cgtcgttgat
       61 ggcgacggca tcgccgccaa aggcccggcc catccagacc cggctgtcgc cgccggtctt
      121 ggtgcccgtc ctggtactgc ccgtaccggg ctgggacgcc gactggcagg ccttcttgta
      181 atcttcatcg gcgtacaatt cgtcgtccac atcgtaaatg gaatcgtagc agtcttcatg
      241 aggaatcccg tagccttcca tatcatcagt ggaaatatcg gcgacctggc gcagcgggtc
      301 attgtcgggc acggccagag acatatcgcc gccgtgaaag accgacgtca cccggtgttc
      361 gaccgatacc gtcccgtcca ggcccaattt ccggctgacc cggtcgcgga gaagatccat
      421 atcgccgatg gcataaggcg attccgtccg gacatgccag gcatgaccgg ccagatcggc
      481 cgcaatcgaa gcgaccgtca ccgaccgccc gccgtcgacg gggatcaaaa tatcattttt
      541 aggaacaata tgccagactt tcatatattt tcctttctat aagcgggcct gtcgcacaaa
      601 gtttttacca ccatgcgcgg tcccttttac gttatagttt ggagtttgca gacaatggct
      661 ttaaatttta accactgaac tgggacggcc cctacatgcc tcccctgctt cccatccatg
      721 ggtaggggcg tccacgtggg acgcccgtcg cacacagtgt gggaacattc gctacctgtt
      781 ggtgaaattc ctacaaaatc caaacggact caccctctac agcggtccgt ttcagcagct
      841 atcctgccat ttcttaccgt catgacctga cggactcgcc acgtgcgagc ccctactaca
      901 tgaaccgatg agttattcct catcggccgt gttgaccgca tcgggcgccg ggacattgaa
      961 gtccatcatg tcgtgcggtg cgttgaggag cgggtcctgc ggttccaggt ccgccgtgct
     1021 gacgacttct tcgctatcgt cgccgggcgt gaggatgatt tcatcattcg gttcataatg
     1081 ggactcgatg gtatcgacat ggcggtcgcc gtcgctgtaa gccacggtcc ggcggatagt
     1141 cacgtcatgg ccgtcgtagc cttcctgctt gcgcttatct tcggtcacac tgtcgtcgtg
     1201 cttcttgatg accttatgag gcaggttctg gagatgcgtc gtttcaaagg acacactgca
     1261 cgtatcggcg tgattgccca ggatatacgt cgtcaccgta ttctgaccgg cgacggtata
     1321 gacgcagacc ggatgctgga aaggattggt aaaggcaaaa tccaggccgt catcggcgac
     1381 agtcgcatcc atgccgacgg gcacataagc ggccggggca aaatgcggtt cccggctggc
     1441 aatgaacaag cccgcccgca aagccgcatt gaacagggtc gtactgacct ggcagacgcc
     1501 gccgcctgtc gactgttcga gcttgttttc aaaatacgac ggtgcctgct tatagccttt
     1561 gtccggcgtc cgcgtgccga catacttgtt aaaggagaaa tccttctggg ccatgacgac
     1621 ggcgtggttg aggcgcgtct gtgccaggcg gatgttgacg ttgcggtccg ggttggtatc
     1681 atcgaaatgg gtcgtatagt acgacaagac cgtgtcgata ttcttgaggt cgtcggcctt
     1741 gatgtccgcc ccttcccggc tgtctacggg gacgtccagc gtgtccgtcc tgcccgtatg
     1801 cagtttggcc gtcacagcat ctttcagctt atctgtattg atgacgatgc ggtccttggc
     1861 atcttcaatg gcgacagtct tctggtcgtc cttgacatag gcataggcat tctgcggatc
     1921 cttatcgtac ttgtcgtgga catcggccag ggccttatcc aatttatcct cgtcaacggc
     1981 aatggccagg ggcacatcct tgccctgcgt gagcgtcgtc accacatcgg ccacgcgctg
     2041 ccagggcagg ccccggcggc cgacgagatg cagttcatca tcgatatagc tgcggtccaa
     2101 cttggctccc agttcgtcca ggtccagcgt cacatcgacg ccgtccgcgg ccaggcgcag
     2161 cttgcagtct tccgcttcct tctcgtggag gtcgagataa ttggtgattt cttcatcagt
     2221 catatcggca atagattgcc cagtgagggg caccagctgt tccggcttgc tctgggccgc
     2281 aaaaaagccg ccgaaaccaa tcacacacaa ggccaagaca acagcagccc gcttcttcca
     2341 ggacggcgtg tccttttccg gaggcaatcc atgcttttcc cggtaccgcc ggcgatattc
     2401 cttcatccgt tctttccgtg ctcgttcttc gtcagtcaca ttctaaccta ccttttctct
     2461 atatttcaat cgcccctggc gcccctcata gggggagctg tcagcgaagc tgactgagag
     2521 gttctcccct gcgatctgca ggccg
//
LOCUS       DAWCYS010000263         2536 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2049_length_2536_cov_4.425232, whole genome shotgun sequence.
ACCESSION   DAWCYS010000263 DAWCYS010000000
VERSION     DAWCYS010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_2536_cov_4.425232"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          632 a          740 c          661 g          503 t
ORIGIN      
        1 gtaaagaaaa cggccaaggc gccgggaaag aaggcgacga cccgtggaaa agggaaagcc
       61 aagacggcag cgtctgaaaa gaagactgct gcaacgagaa agagaagaac tgggcgtcct
      121 gccaaaaagg aagcagacgc ctgatacggt acttagggaa agtgggggac atacgatgaa
      181 tgtattattt gtggcctcag aagcagtgcc atttatcaaa acaggcgggc tggccgatgt
      241 catgggcgca ctgcccaagg ctttagccaa gaagggcatg gacgtccgcc tggtcattcc
      301 gaaatacagc cgcatcgctg aagaagcgaa gaagcagatg aaagaagtga cgacaggcat
      361 cgtgaacctg gtctggcgcc agctctacta cggtgtcgaa gaaatcaagc aggacggcgt
      421 gaccgtctat ttcatcgaca acgcatggga gttcaagcgc gacggcctct acggctacgg
      481 cgatgacgcc gcacgcatcg cctatttcgg ccgcgccgtc ctgaccatgc tgcccaagat
      541 cggcttcaag ccggacatca tccactgcaa cgactggcat acgggcctca tgggcgtctt
      601 cctcaaagaa gatttctggc acgatgattt ctacaaggga atcaaagtca tctataccat
      661 tcacaacctg aaataccagg gcatctatcc gccgtcgatc atgcgcgaca tcattggcct
      721 gccgcaggaa ctcttcgaca acggcagcct ggaatgtgac ggctgcgtga actacatgaa
      781 gtcgggcatg gtctacgccg accacatcac gacggtcagt ccgtcgtatg cccaggaaat
      841 cacctatccg tactttggcg aaggcctgga tggctatatc cgcgacaatg gggaaaaggt
      901 gacgggcatc atcaacggcc tggacatcga cgtctacaat ccggaaatgg acccgtatat
      961 caagcgccag tattccgtcg ccaacgcccg ggctggcaag cgggaaaata aagatgccct
     1021 gcgggccgaa ctgggcctgc gcatcaagcg gggcgtcccc atgatcgcca tggtcacgcg
     1081 cctcgtcgaa gacaagggcc tggacctggt cacgcgcatc atggatgaac tcgtcgacga
     1141 cgacgtccag ctggtcctgg tcgggacggg cgatgccggc tatgaagaag ccttccgcga
     1201 actggcccgc cgccatccga ccaaggtatc ggcgaacatc accttcaacg aagccctggc
     1261 ccacaaggtc tacgcctctg ccgacatgtt cctcatgccg tcgcggtacg aaccgtgcgg
     1321 cctgagccag ctcatcgccc tgcgctacgg cgccattccc atcgtccgcg aaacgggcgg
     1381 cctcaaggat acggtccatc ccttcgataa atacagcaaa cagggcaatg gcctgacctt
     1441 cccgaacttc aacgcccacg aactgctgtt cacgacgaaa cgggccatgg gttactatga
     1501 agacacggcc ctgtgggata tcctcgtccg caacgccatg accagcgact acagctggga
     1561 ccgctcggcc gaagcctacc agaacctgta cgaaaaagta ttacaacaat aaaagacagt
     1621 ttgtagttca gagtttgtag tttgtagaaa ttggctttgc atttccagct attcactaaa
     1681 aactaaagac tgcaaactca aaagggactg tcgcatgaag gcttctgcca ttatgcatag
     1741 tccctttgac ttatagtttg cagtacggag tttttagttt gtagcagatg actttaaatt
     1801 taaaaccatt cactacaaac tgcaaactta atactcaaaa agaggctgtc ttcatggaca
     1861 gcctctttta ctttccccaa ccacgaaagg accgcttatg gacttacgat tgtaccacaa
     1921 ctctcacgaa ttctattacc gctcgtcttt tggggccgtt ccttccggga gccggctcat
     1981 cctgcgcctc cgtgccagcg gcagcgatgc tcagaactgc cgggcccgca tccgcctgtg
     2041 gcagatcaat gtcggcgaat cctttgtacc catgagttat gaagacggca tcttcacggg
     2101 catcgtcacc attccgccca agggctgtct gctctggtat tatttcatca tcgacgatgg
     2161 ccataggacc ttctactatg gcaacaacta cgaccgcctc ggcggcgaag gggccaaata
     2221 tcccgacgtg ccgccggcct atcaggtcac ggtctacgat aaggacgccc atacgccgga
     2281 ctggttcaag cacgccatcg tctaccagat cttcccggac cgcttccgcc acggtaccca
     2341 tacgtcgggc cgcctctacg gcaagaaggg cgccgtcatc cacagcgact gggacgatgt
     2401 gccctattac tgcaagcgcg aaggccaggg cgacatcgtc tattacgacg atttcgtcgg
     2461 caacctcgcc ggcatcctgg caaaattccc ctatctcaat gagctgggca ttactgcgat
     2521 ttagctcaac ccgatt
//
LOCUS       DAWCYS010000264         2502 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2081_length_2502_cov_5.770740, whole genome shotgun sequence.
ACCESSION   DAWCYS010000264 DAWCYS010000000
VERSION     DAWCYS010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2081_length_2502_cov_5.770740"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          550 a          654 c          640 g          658 t
ORIGIN      
        1 ttttcaatcg tctttacgtc agccatatga gccatagggc agctggccgt cgcattgggc
       61 atgaggacct tcttttccgg gttgagcatc ttggcacttt cccccataaa gtcgacaccg
      121 gcaaagacga tgaccttggc atccgtttca atcgccttct tggccaggcc aaaagaatca
      181 ccgacaaaat ctgcatattc ctgaacttca tcaggtacat aatagtgagc aaggataata
      241 gcctgcttct cttttttcag agctgcaatc tcatcttcga tatacaccaa acttcatcct
      301 ttcagggaag aggttgcccc ataaagacac cttcatgtga caacctcttt tatttaaagt
      361 acataacggc tcatcatccc ttgcgggata gaccgttttt ttgtttaccc tattataccg
      421 tatcggtaaa gatttgacaa cactactgtc aagacattgc aagatgtgaa aaagaggctg
      481 tcgcattaag acagcctctt tttagtggtt agccgctagt ggctaggggt tagtggatgg
      541 cttgaaattt aaaggtttgc tctgaaaact acaaactccg tattacaaac tatcacctta
      601 tatcaattta ttttgcttgg caatttcagc gactgcttcg atagaaagtt tcatgtaccg
      661 ggcaatttgc tcaaaggata gaccatcttt aatcatttcg agcgctgctt cccggtcttt
      721 ccgttcagaa tttcgttttt cttgctcaac gccttttttt atgccctttt ttatgccttt
      781 ttctataccc ttttccaagc ctttttccat ggctctttca tagagtcctt cacctaagtt
      841 gcacatagtc gtgacatcct tcttcccttt agccgtcaaa tcaatgtcat attcatcatg
      901 taaggctgtc agtagatctt ctgatttcat cttttttgat ttaacctcat tgtagcaaat
      961 acccccactt tgtataacac gaataaggtg tcgtcttatg atttttccat atacaaaagg
     1021 gacctgccac gacggtatgg caagtccctt caggatatca agatttcagc cacgaaccac
     1081 tagccactaa ttactaactg ctagccccta cccgtggatc aagtcgagca tggcgtggag
     1141 tttgtcgatg gtgtctgccg cttcctggcg gacttcttcc gggtcgcggg tgatgcgggc
     1201 gacgccgctt tccatggcag ctttggcgac ggctgcggcg acggccggag cgacgcgttg
     1261 gtcgaaggcg tcggggatga cgtggtcttc ggccagttcg tcatcgccga ccagggaggc
     1321 gatggcgtaa gacgcagcca gtttcatgtc atctttgatg ctgcgggccc ggacggccag
     1381 ggcgccgcgg aagatacccg ggaagaccat gacgttgttg atctgattcg gtgcatccga
     1441 ccggccagtg ccgacgactt tggcaccggc cgctttggca gaggcgtagt ccgtttccgg
     1501 gatggggttg gccatggcga agacgacggc atcgtcggcc atggcctgga tgatttcctt
     1561 agtaaaaata tgcggtgccg atacgcctag gaggaggtcg gcccctttga cggcatcagc
     1621 cagggtcccg tgggcatggg acggattggt cagggatgcc agttccgcct ggaccgggtt
     1681 cagcccttcc atgccagcgt agagaatccc cggacggtcg accatggtga cgttccgagc
     1741 tccggcgcgg accaggaggc ggccgatgga agcgccggca gcccctgccc cattgatgac
     1801 gatgcgggct gtcttcaagt ccttatggac atagcggaag gcaccgatga cgccggccag
     1861 ggcggcaatg gccgtgccgt gctggtcatc atggaagaca gggatgtcca tttcccgttt
     1921 caaggtctgt tcgatttcat agcatttcgg cgaagcaata tcttccaggt tgataccggc
     1981 gaaagacggc tgcaaacgct ttaccgtttc gatgaattct ttcgggtcct tcgtatccag
     2041 acagataggt acggcattga cgccgccaaa gaccttgaag agaatggatt taccttccat
     2101 gaccggcatg gcagcttccg ggccgatgtc gcccaggccg aggacgcggg tcccgtccga
     2161 tacgacggcg atcatattgc cacggcaggt caagtcgaaa gacaaatccg ggtccttagt
     2221 gatttcccgg cacggctggg caacgccagg cgtatagaga atactcaggt cgtcgtgggt
     2281 cttgaggact tcccggggaa tggtttcaat aacgccgtgt ttgccgcggc ggattttcat
     2341 ggcttcttca tcgaaattca tgacgctaaa actcctccta taactgttta tgcataaaaa
     2401 atggcctggg gttctgtaaa ttccaacatc ccatctttgc cgccttcacg gccgatacca
     2461 ctgtccttaa agccgccgaa aggcgctgtg acgtcgcgcg gg
//
LOCUS       DAWCYS010000265         2494 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2086_length_2494_cov_5.216892, whole genome shotgun sequence.
ACCESSION   DAWCYS010000265 DAWCYS010000000
VERSION     DAWCYS010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_2494_cov_5.216892"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          649 a          654 c          682 g          509 t
ORIGIN      
        1 gaaaagccct gttccatgcc cgatggcgta tagcccaggg cagagccgaa gaaatccgta
       61 tcggccactt cttcggcatc ccattcgccc caggccgtgc cggcagctaa caagccgagg
      121 ggcgtggcgg cgatgagggc ggccaggagt ccgaagaggg acttgctcga cgacggggcc
      181 tggaccgcat ggaagaggtc tggcgacgtc ttgcgcagat aggtcaggat gcctgtcgtg
      241 aagacgactt ccgccaggcc gaacaaggtc aggtggccga tcatcatggc cggaagggaa
      301 atccacaggg gatacgggca gtacagggcc tgaccggcag catcggtgaa cagcaggggc
      361 tggatgccaa attccacggc ggcacagaag gcagctgcgt tgatgccaac ataagcgccg
      421 atgccggcgg cgaccgtatc cgacgagatg accttcttca ggccgtgata gatgaaatat
      481 cccgtaaagg gcaggacgaa agccatgttg aagcagttgg cgccgaaagc caggatgccg
      541 ccgtcaccga agaacagggc ctggatgagc agggccacgc tgacggccag acaggccgcg
      601 tacggcccga agaggatggc aatgagcgta ccgccgacgg cgtgtcccgt cgtaccgccg
      661 ggcaggggga tattgaacat catggccagg aaggaaaagg ccgcagcaat gccgaggagg
      721 ggcagctttt cacggggcac cgtttccttg accttgcgga tggacagcag ccagataggg
      781 accatgacgg cgccgaggac accgcacgtc gaagggctca ggtaattttc aggtatatgc
      841 ataatgaaac ctcttttcct tagaaattta actatattac tattttacta aaaaagcccg
      901 gccaatataa gcccatgggg gggataaaaa ataggccttt acctgacgct ctggcagata
      961 ctcgatggcg catccatgct atggtataat ataaaataaa tctatattag aaagagagta
     1021 taaacgatga ttgattttca tgaaattctg gaccgcatga atcaaaacca gcggatcaac
     1081 ttcgataaag tattccagcg catggcccgc cgttggcagc aggaaggcgt caagccgcgg
     1141 gtcctgctcc attcctgctg cggcccgtgc agtacggccg tcctggatag gttgaccggc
     1201 gtggccgatg tgacggtcta ctatttcaac cccaacatcc atccggaagc ggaataccgg
     1261 cggcgggaac tcgtccagaa acagttcatc gaagcctata acgaaaagac cggccatgat
     1321 gtccatttcc tggccgctcc ctatgagccg gagacatact ttgaagccgt caaaggcctg
     1381 gaagatgaac ccgaaggcgg cgcccgctgc cgcgtctgct tcgtccagcg catgaaggcg
     1441 acggccgaaa aaatgcagga actgggctat gattacttta cgacgaccct gaccgtcagc
     1501 ccccataaga attcccaggt catcaacgaa gtcggccggg acatcgaaga ccagtacggc
     1561 tacgcctatt tgccgacgga cttcaaaaag aacaacggct tcctcacatc gacgaaaatg
     1621 accgaagcct accacctcta ccgccagccc tactgtggct gcatcttcgg tgcccgcgac
     1681 cagggcctgg acttagaaaa aatccgccag gacgccgacg cctttctggc gaaaaatggg
     1741 tagggacgca ggccgcagtt cgctggtcgc aggtcgcaaa aataacccca atcggggcat
     1801 tcgtaggggc tgtcctttaa cctctcagac cgcctttggc ggccagctcc cctataaagg
     1861 gagccttgtg cgtcccgttg cgagatttca tcacagcctt ttcgagtata atggcaggct
     1921 tttacgagcc acgagccacg aaccacggcc ttttccacga gccgagggtt actcgaggcg
     1981 atgtcattat tctgagtctg tcgccgcgaa gcggcctaag tccgaggcgg cctgcgaact
     2041 gcgacctgcg gggccgttgg ccccaaaatt cgatgaacga cctacgacgc acgccttacg
     2101 cctggccgtc aggcccctat cttacccaaa aatctcagaa aaatttttgt tttgctatat
     2161 tcatattttt acaattaatt gtattaaaaa tatattaaaa atattaatac aaaatacggg
     2221 tcaacgactt agcaatggct tatgaatggc tatatatggc tttattttgc attttttgta
     2281 tgctataata agataacagt tttgttacaa atatgtcatt ttgttaactg cataagaggc
     2341 atataatgca aagaaagggg ctgtcttttt atgaatcgaa cgtataaggt catttggagt
     2401 aaagccagac ggtgctatgt cgtcgtatcg gaattggcca aaggccaaca caagtcgtcc
     2461 agtaagagcg aacacataag ctctgatgtt cacg
//
LOCUS       DAWCYS010000266         2492 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2088_length_2492_cov_5.682807, whole genome shotgun sequence.
ACCESSION   DAWCYS010000266 DAWCYS010000000
VERSION     DAWCYS010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_2492_cov_5.682807"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          566 a          657 c          777 g          492 t
ORIGIN      
        1 cgcgggctgt cgttgacgta gacgttgccc gacataacgg cttctgcctc cttcgaggct
       61 tcttctttgt gctgtgccca gacggcagcg ttgaggccat aaggcgtatc gttggcaatg
      121 gccagggctt cgtcggcggt atcataagcg atgacgctga ggacggggcc gaagatttct
      181 tcgcgggcaa tggtcatgtc attggttacg tccgtaaaga tgacaggctt gacatagtaa
      241 ccgtggtccg gcgcatccgg acggccgcca gccagaagtg tcgccccttc ttccaggccc
      301 ttggcgatgt aggcatcgac ggtctgccac tggtcatacg atgcgacagg accgattttc
      361 gtcgtttcca gagccgggtc gcctacggta tattccggca gtatcttcag catgagctgt
      421 tcaatctgtt ccttgtcatc ccgcgggatg atcaggcgcg acagggccgt acaggtctgg
      481 ccactgttga ggaaaatgga attgaagcac atgcggatag ccttttcgta gtccgccccc
      541 ttcaagagga cgaaaggcga cttgccgccc agttccatac tgatgcgctt catcgaagct
      601 aaggcgcgct gagatacctt gatgccggct gccgtcgacc ccgtaaagga taccatggcg
      661 atattcgggt cgtcgcagat ggcattgccg acctggctgc cccggccggc gacgagattg
      721 aagacgcctt tggggaaacc ggcctggtca aaggcttcgg ccaggtagta cgccgtcacc
      781 ggcgtgtgct gactcggctt gaggacgact gtcgccccga cgagcaaggc cggtacgact
      841 ttctggataa tctgccctaa aggataattc cacggcgtga tacagccgat gacacccaaa
      901 ggccggcggt agacgacgga ttgggacaga tgttcgacca gtggcaattc cggtgccaag
      961 tcgatatacg actgggtccg cacgaactgg tagccaatat ggttggtcct ggcaaaggct
     1021 acaggcgccc ctaattcctt gacgacggcg tcgacgaact gcggttccaa tctgatcatc
     1081 tggaccagca ggttcttcat gaaggtgatg cggtcttcca gcggcatagc cgaccaggcc
     1141 gggaaagctt tggcagccgc ctgggcagcg gcagcgacat catcggcacc agccgcggcg
     1201 acagtggcga agatttcttt cgtcgccgga ttttctacgt caatatattg gccagacaag
     1261 ggcgcaaccc actggccatc gatatatagt tttttcaaat ccatgaaatt accccctttt
     1321 actcgagatt attctttaat tatagagata agtcattgat tatgcaagtc ggggaaaaaa
     1381 atagactgcc taaaaggcag tctattttta gtggcgcccc ctacagactc cataccacaa
     1441 actatcttat ttcgcttcgg cttctacggg ttctgaggcg gatacgaaat gaccgatgat
     1501 ggagccgccg atgaggccga tgataaaggg gacgatccag ccgaaaccgt atgtggacag
     1561 gggcgtagcg gcgacgatgg ctttagcaaa gcccaggctg ctcagacccg gcagggattc
     1621 caaaagggcg aagagcgtga ccaggtagac agagcctttg taagcaccgt tgttgggcat
     1681 gaagcggccg atgatgccca ggaccaggag ggccaggcac ggcggatagc agacgccgaa
     1741 aatccagccg atgtaggtga cgataccgtc gacgccgagg agggccgtca gggccgacaa
     1801 gatgcagcag gcgacggcga cttgcttata gctgatgcgg ttgcccgtag ccgtgctggt
     1861 gaattcggca acggcggcaa tctgaccgat ggctgtcgtc aggcaggcca ggacgacggc
     1921 gatgcccatg ggaaccatga cccacgtgcc gacgcgctgg atgatggcga ccaggagttc
     1981 cgcacggccg atgttctgcg catagtcacc gctgacgcag gcgcccatgt agagcaggct
     2041 gccgtaaatg atgagcagca ggacgaagga aacgatgcca cagtagatga gcactttatt
     2101 gcgctggccg acggtcgtat agcccttgcc gtaaatggag ctgatgaaga cagctgccat
     2161 gatgaagctg accaggacgt cgcccgtgtt atagccgccg aggaaggcat tgacgaaggg
     2221 atggtccaca gccggagcga cgggtgtccc caggggattg aagatgccga cgaaggcgac
     2281 gatgaccagg atcagggcca ggagcggcgt caggtatttg cccatgcggt ccatgacgtt
     2341 gttctggtcg gcaacaaaga agaagctgat gacgaaatag gcgatgacca gcgggatgaa
     2401 cggtgctgcc ggcaggttgc cctggatgcc gagctggatg gccgtcgcac acgtacgggg
     2461 aatggaaacg aaggtacaga ccagcatgag gg
//
LOCUS       DAWCYS010000267         2456 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2131_length_2456_cov_5.218659, whole genome shotgun sequence.
ACCESSION   DAWCYS010000267 DAWCYS010000000
VERSION     DAWCYS010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2131_length_2456_cov_5.218659"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          622 a          616 c          752 g          466 t
ORIGIN      
        1 gataactgag tccatttttt tcgatgaggc tattatgtta cagccccttt tttaggggtt
       61 agtggctagc agttagtagt tagcggatgg ttttaaattt aaagcttttt ctctgcaaac
      121 tccatcctct ttttttcata tgaaacctct ccggcccttc gggccacctc ccctataatg
      181 gggaggcttg gcgggcttgc cacgtgcaag tccctacagg acggagctta cgaactacga
      241 attgctgctt tctttctgca agaggctgtg tttgcgtccg taaaccatgt agatgatgag
      301 gcccaggatg gtccagcctg tgaacagcat cagcgtatgc ggcgacagtt cggagaagat
      361 gaaagcgcag cagatgaagc ccagcggagc gatgacgtgt acagccgggc agcggaatcc
      421 acgcggatgg tccggataga gccgacgcag gacgaggatg ccgacgcagg agcagaggaa
      481 ggcgaaaatc gtaccggcac tgcatgtttc agcgacaatg tgaatcggtg tgaaaccggt
      541 gatgcaggcg acgatgacgc cgatgacgat ctggatgata tacggcgtat ggaacttcgg
      601 atgaaccttg taaaggctca tgggcatgag gccgtcacgg ctcatcgagt agagagcccg
      661 gctctgagcg aagagcatgc ccaggacgac cgtcgacagg ccgcagatag cgcccgtacc
      721 gacgatggcc gaaccgagat ggaggccgat gtattccagg acgaaggacg ccggtgctgc
      781 cgtatcgagc atgggatacg ggacgacgcc ggtcatggcc gcacagacgg cgatgtacag
      841 gaccgtacag atggccaggg ccgaaatgat gccgataggc atgtcgtgtt tcggattctt
      901 cgtttcttcg gctgccgtcg acagggcatc gaagccaagg taagcgaaga agagaacggc
      961 cgtcccggca aagacgccgt ggatgccata aggcaggaac ggctgccagt tcatcggttc
     1021 gatatgcggt gctgccagga agaggaagag gaaaatcgtc ccgatcttga cgtacaccag
     1081 gatgcggttg acgtgagcgc tttcgcggac gccgacgacg aggagcgccg tcgccaggac
     1141 gacgatgagg accgccggca gattgatgag tccgccgtca gccggcacgg ccgtcaaggc
     1201 tttcggcaag tcgatgccgc aggaatgaag gataccgacg aagtaggccg accagccgcc
     1261 agctacggcg ctggcgccga ccgagtattc caggatgagg ctccagccga cccaccagcc
     1321 aaagatttca ccgagcgacg cataggcgta ggtgtacgaa ctgccggata cgggcaggaa
     1381 cgaagccagt tccgaataag caagacagac gaacgcgcag gtaatagcgg cgaagacgaa
     1441 ggataacatc aggccgggac cggcgtattt cgctgcgccg ataccggtga ggacgaaaat
     1501 ccccgtcccg atgatgatac ccaggcccat gagggtaata tcgaaggcgc cgagagcctt
     1561 gttcaatttc ttttcctgcg tatgggccaa caagtcatcg aaggatttgc ggcgtaaaat
     1621 attcataaac atgcccccaa tccttactga ctagctttgt ttgaagaaac agcagctctc
     1681 caaatgaaac cgatgatgaa cccagcgatg gtgaagctga tccagcccat gcccagcgtg
     1741 tggagcggta ccatggtatt gaagatatta tcgataggtc ctaaggaaat tttcgccgtc
     1801 tggagccctg tgaccaaagc cgggatgagt gtcaggccga tggtccaggc gtagacacac
     1861 tgccgtccat caaagagatt gttgaggaag gtcaggatga tgatggcgac gacgagagga
     1921 taaatgaaca tcaagaccgg aatagcggcg acgatgatgg tcttcaggcc gaacatggcg
     1981 acgaagaagg aaatgaccgt aaagatggtg acccatttct tatagccgat attgccgacg
     2041 agttcattga aatacgtagc gcacgacgta atcagaccga tggacgtcga taagcaggcc
     2101 aggagtacga tgacggccag gatgacagcc ccgacattac caaagaggat catagcgctc
     2161 ttggaaagga ccggtgcgcc tgtttcctgg ataccgaaag cggcgacgct gtcggcaccg
     2221 atcttggcga tgaagatgta gacaaaagcc aggcagctga cggcaatgag gccggcctta
     2281 aagacttcct tagtgatagc cgccggttcc gtagccccgt cttccatgac gaattcgatg
     2341 accaggatgg cgaagaccag cgaggcgata gcatccatcg tgttgtagcc atcgaggatg
     2401 cactgtacgg cagcgatgct ggccgttgca taagcagcgg tcggttcctg cggcga
//
LOCUS       DAWCYS010000268         2449 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2143_length_2449_cov_4.395572, whole genome shotgun sequence.
ACCESSION   DAWCYS010000268 DAWCYS010000000
VERSION     DAWCYS010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2143_length_2449_cov_4.395572"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          514 a          729 c          675 g          531 t
ORIGIN      
        1 acgtcaggaa aacatccaga agctgacgga taaaaaaatc aaggtcatcg acgaaatcaa
       61 agcgaaaaaa gaaaaagaag tcatggccgt atgatggata tacggatttt tattggcatg
      121 ccgttacggt atgccaaatc tgtattacag gaaaaaaata ttccttttgt catagagcgg
      181 acgctgtcgc ggagccactt tttccactgc gaccctgagc agacctatgt catccgcgcc
      241 cagcggcggg atgacggcac agtctgcctc ttggtcaacg acagcctgga gatgagcgga
      301 tccgtagcgg cagccctgcg aaatggagac gtacagcatg attaactttt ttggcaaaaa
      361 gaaggaaaca gcaggctccg tgtccgaagc catgctggat ccgaacaaca ttccccgcca
      421 cgtagccatc atcatggacg gcaatggccg ctgggccaag cgcaagggcc tgccccgcag
      481 ttacggccat cgcgccggtg ccgatacgct caagcgcatc gtcgtcgctg ccgacgacct
      541 gggcatcaag gtcctgacgg tctatgcctt ttctacggaa aattggaagc gccctgatga
      601 agaagtgtcg tacatcatga agctcatgaa gagttacctg agcaagaatc tcctggaact
      661 caaggaacac aacgtgcagc tccacgtcat cggcgatatg aagcggctcc atccggacct
      721 gcaggatacc ttttctcagg ccgaagccga aatggccgcc aatacgggcc ttatcctcaa
      781 cgtcgccgtc aattacggcg gccgcctgga aatcacccag gctgcccgca aagtggcaga
      841 agccgtccgg gacggccaga tccgccccga agacattagc gaagcgacgc tggcccagta
      901 tctctatacg gagccggaaa atgacgtcga tctgctcatc cgccccggtg cggacaagcg
      961 ggtcagcaat ttcctcctct ggcagatggc ctacgccgaa ttctggtaca cccagctctg
     1021 ctggcccgat ttcagcaaag ataccctggt cgaagctatt ttgtccttcc agggccggga
     1081 acggcgcttt ggcggcttaa aataaatcag ggaaggtata tgatatgttg gcaaaacgaa
     1141 tactgacagg catcgtcggt ggtgccttta cgatttttgt gatttacgaa gggaactggc
     1201 tgtttttcct catgatggcc ctgttatccc tcgtcgcctg gtatgaatac ggcaacctgg
     1261 tccacaagct ccacgccgtg acggccgaca agtgcggtgc cgtatggctc gtcgccatgc
     1321 tggccgccta ttggttcggc agcaccaagg tcatgctcgt cctggccttg ggcctccttt
     1381 tctggctcat gctgcggacc gtctttttcc acaacaagat taagcccgta gacagtgcct
     1441 ataccttgta cggcctgctc tatatcggct gtggctttct ggccatgctg gccctgcgca
     1501 gcggctctgt ggcttccttc atgggcagca gcttcggcgc cgtcatgttg gaaccggcgc
     1561 ggttcttcgt ctttctcctc atcttttcga cctgggccag cgatactttt gcctttgccg
     1621 tcggcaagat gatgggcaaa gtcaagctct gcccggccat cagccccggc aagacccgcg
     1681 aaggtgctat cggcggtttc gtcggtaccc tggccgtggc catcatcttt tcgctcatct
     1741 ttaaattctc cgtcctccac gccctggcca ttggcgtcat catcggcatc atggctcctt
     1801 taggcgatct ggtcgaatcc atcttgaagc gcgtctgcgg cgtcaaggat tcggggaaca
     1861 tcatccctgg ccacggcggc gtcctcgacc gcttcgacag cctgctcttt gcagcgccgg
     1921 ctatgtacgt ctatttgatg ctggtcattt aaaggagggc agggaatgaa ggatattttg
     1981 attttaggca gtaccggctc tatcgggacc caggccgtcg acgtcatcga cgcccatccc
     2041 gaggccttcc gcctggccgg cgtcgccgct tattcccatt ggcaggaagt ggcccgccag
     2101 gtccgggacc atcaggtgaa atgggcctgc atggtcgaca aaaaggcggc tgatgaactg
     2161 cgccgtgccg tcggtccggc gtgtactgtc ttttccggca gtgacggcct cttgaaactc
     2221 atcgatgaat gcgacgccca gatcgtcctg acgtccctcg tcggcgccgc cggtatcgaa
     2281 ccgacgctcc acgccatcga gaaggggatg gacatcgccc tggccaacaa gcaaaccctc
     2341 gtcgctgccg gtgaactggt catgaaggaa gccgcagccc gcggcgtctc catcctgccc
     2401 gtcgacagcg agcacggggc catcttccag tgcctccagg ggcgccgcc
//
LOCUS       DAWCYS010000269         2446 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2144_length_2446_cov_5.868256, whole genome shotgun sequence.
ACCESSION   DAWCYS010000269 DAWCYS010000000
VERSION     DAWCYS010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2144_length_2446_cov_5.868256"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          538 a          647 c          721 g          540 t
ORIGIN      
        1 aaactataaa gtaaacgggg ccgcgcatga tggtagaagc cttcgtgcga cagcctccca
       61 cggatcacca ttcactattt cgccggtgct tcgatagggt cgtaatcagc cggtttgtcc
      121 gtaatggatg cttcgtcgat ggccgacggt accgggtcga agccctggcg gaacagggcc
      181 gtgatggcag cgagggacgg cgggatgatg gtgtccatga ggagatagcg ctagcggaaa
      241 aggttgcgct tctgcgactt gatttcgccg tatttgcgcc gtctttcttc gataggcagg
      301 tggtaatacc acggttcctt gccatccggt gcggctcctt cgccgatcca gaattcattg
      361 aaattggtct tcttcgagcg gttgccgcgc agcatatggc tgttggtata gaagccttcg
      421 tccgtgatga catatatgtc atggatgccc aaggtctgga cgaagatgcg catgagatag
      481 aggatgaaat tcttcggccg atagccaaag agcttcttgg tcaggacctt ggtcgtttcc
      541 aggccgtgct ttgagccctg gatggtgccg atgtacacgg acggcgtccc gtcaacattg
      601 taatcgaaac gaaaattgaa atgatagatc atctggcctt catgatagag gtagagcgac
      661 aagaagcctt ccttgcgctg gcccgtatcg aagatgagcc gcgcttccag gggcagcgat
      721 tcgtccggcg aagtccacag ggtatagccg ttcccccagt agagttcgtg gatgacctcg
      781 tcagaaaaga aagtccgcaa gatagtgaaa tgatgtttca aggcatcgag acgctggcgc
      841 atggtcgaat tcttgaacaa gaagacgcgg gtcatgactt cctggaaatc cgggtcatgc
      901 tgctccaaga agcccttcat cggttcataa tggtcaaaga aatcgtagag ctcttcaaat
      961 aaagcccggt tggacacggc acgggcaaaa aaaacgacag cccgcttgtt ttcctggaaa
     1021 ttccggggcc cgtatataag gcgcgataag tgccagaaat aggaaatgct gtgcatggaa
     1081 tacctcctat caagtacaaa gctaatagta ctattatacg ggcaagacaa gcaggctgtc
     1141 aatgtgggat gcgtggccag tggctagctt ctatctaatc cagcttccgc acgatattcg
     1201 ccgggccatg ggacaaatcg cggacgccgg tgatcatcat ggcatccatg agctggcgtt
     1261 tgagcgtatc caggaggagt ttgacccctt cggccccacc tccgacagca gcgacggcag
     1321 ccggtctgcc gatgaggacg gcgtcggcgc cgagggccag catcttgagg atgtcttcgc
     1381 cgtgacggat accgccatcg acgaaaatgg tgatccgccc tttgacggct gcggcgatat
     1441 caggcaggac atcggccgta ccggccatgc cgtcgaggac gcggccgccg tggttgctga
     1501 cgacgatgcc ttgggcaccg gcttcggcac aggagacggc ttcttcggcc gacatgacgc
     1561 ctttgacgat gaagggcagt ttgatagcct ggacgacttc ggcaatgtca cgggcactct
     1621 tgggccctac gggctgaccg aaaatccgca tgttaatcag cgtcgccgca tcgatgtcgc
     1681 tggcaacggc aaaagcgccg gcttcttcgg cctgatgggc tttctcgatg atctggtcgt
     1741 tgctgcgggg cttgatagtc gggatgacga tgcccggccg tttcttacaa gatgcaatgc
     1801 cggatgaata catatacgga gcaccgctgt caccggtaaa gcacagcgta cccacatcca
     1861 gggctgcctg agtgacggct tcgtcatact cttgttctac ttccgccggg tcgccgtcta
     1921 cctgggcatt gaggacgatg ccgccgaccg gcgccaggag taccggcata ctgagccgtt
     1981 ttcccataag ggtcacagcc gtatccgggt cgtcgacgcc agacatgctg cgcatgacca
     2041 ggccgtattc ctggagcgct tcgacgttac gcatgaaaga ccgtcccgtg cgcaggccgc
     2101 cgaaaccggg aatcatgccg acgcaggctt tgccgttgca caggggacag acatggcagc
     2161 ctttcatttt ttcgcgtgct ttgttcctta cttcttgtaa atccatgata atcacttcct
     2221 aaataaatat tttattgtca gcccggccct gataggtcgt ctctaacatc ttgacatggg
     2281 ataaaaaggc agggtcgtcg aaatacttgg cgccatgcgg gcagttgcgc acgcagcgct
     2341 ggcacttgat gcagatgcca ttgacgacgg acggctggtc cgggtcgatg gagcccatgg
     2401 gacactggcg ggcgcagagg ccgcaggata cgcatttttc caggtc
//
LOCUS       DAWCYS010000270         2442 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2147_length_2442_cov_4.878090, whole genome shotgun sequence.
ACCESSION   DAWCYS010000270 DAWCYS010000000
VERSION     DAWCYS010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2147_length_2442_cov_4.878090"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          586 a          726 c          612 g          518 t
ORIGIN      
        1 gatctggaca tcgacgagcg accagtaatc gagaccggcc cgttttaaat gcttgtcatc
       61 aatggaaaag cccaggggct tgaccaaatg cagcgtaata tgattggctg cgcataaacg
      121 ggcgacattg cccgtattgc cggggatttc cggctctacc attacgatat gcatacttat
      181 atcactctcc aagggatatt gtacaggatt tatgcaggaa gtacaagagg aacattgcgt
      241 ttccgacctt tttatgatat acttataaat aatagttatt atttaataga aatgtaaagt
      301 ttctgtttat tttagaaaga ggtgtcaatt attatggata actttgacaa cattgcccag
      361 taccccatgt atttcgctcc cggctgtaag ctgctgcagc tgcagcccca gatggtttcc
      421 gacgtctacg attacctgcg tcagctcttc ggcagcaaaa tcaaactgta tacacgctgc
      481 tgcggcctcg acgatgccaa ccagcacaac gacgaagccg tcttcatcac gctctgcgac
      541 acgtgctaca agatttacgg cgaaacctac gccaacctgc acatgcgcga cttctggaac
      601 gtctatgacg aatacaagga cgtctatccc ctgggcgaaa aagaaggtga actccgcaag
      661 accttgaaga acaccttctg tggctccctc ccgcaggaac acgtcaagag ctggttcgaa
      721 gaatggaaga aatggagttt gaacagtaca gaaaaagact aattcgtctt taaggagcag
      781 aggctatcat gaaattcact tactacggtc attccgcatt cacactttcc gatgacgtga
      841 cgacgattct ttttgacccg ttcattacgg acaatccctg gacccaggtc tgtccgacag
      901 acattgcctg ccagtatatt atcgtcaccc actgccacgg cgaccactac ggcgacaccg
      961 aagccattgc caaagccaat gacgcgacgg tcatcagcac cgccgaagta gccggcaaag
     1021 cgggcgaagc aggctgtaaa gcccatgcca tgcatgtcgg cggcagtgcg gattttccct
     1081 ttggcaaagt acggctgacc ccggccttcc acgggtctgg cattgccgga gggctggcct
     1141 gcggtgttat cgtcgaattc ggcggcaaac gcatctacta tgccggcgat acgggcctct
     1201 tctcagacat gaaaatcctc gaccgtttcg gacaaatcga ctgcgctatc ctgcctatcg
     1261 gcgacaactt cactatgggc atggatgacg ccgccctggc cgccctctgg gtccagccgc
     1321 gcttcgtcat ccccgtccac tacaagacct ggccgatcat cgaagcagac cctcaggaat
     1381 acaagaagct cacggaaacg aaatacaaca tccccgtcca ggtcgtcgac ccggggacga
     1441 cgtatgaatt ctaatcatga tccttcctga tttctacgga cgtttccgct gcaaagccag
     1501 cgcctgccaa cacacgtgct gccggggctg ggacatcgac atcgacgacg tgacggccga
     1561 ttactaccag cagctcgaag gccccttagg cggggccatc cgccaacata tcgccgaagg
     1621 accggacggc tggcatttct gcctcacggc ggaagggaac tgtccctttc tccgcccaga
     1681 tggcctgtgc cagctcatcc gccaggccgg ggacgatatc ctctgcgata tctgcgctct
     1741 ccatccgcgt tttttccagg tcgtcgaaaa cggcagcagc caggccatcg agctcggggg
     1801 cgtcggcctc tgctgtgagg cggcgtgtga actgcttctc agcgaaacgg ggccactcac
     1861 cttctgcgac agtgaaacag gccgctccat cggcagcctg gcccagttgc tgaaatggct
     1921 ggactatccc cttccccgca aagggcttac gtatgaaccg gccacggctg aaaaggacca
     1981 tgaacggctc ctggccgtct tagaccgtac cgaaccgatt gatgatgcct ggacggccca
     2041 gctgaaagcc ttacaggcgc agacagcgaa ttcagctgat tccacagccc tcaccgggaa
     2101 caccgggacg gccggacgct atcagcgcat atacgattac atcttctacc gccagctgga
     2161 aacctttgcc gacgtcccgt cagacttcct ggccacctat gccggacgca gcacggactt
     2221 catcgccctc acagccgccg ccacgggcga tctgcccgaa gcggtacgcc gctggtcgga
     2281 acaaatcgaa tatgatacgg acaacgtggc gctcctcaag aaagcggcag cggccggaga
     2341 attttgactt ttccggcatt tcgtgacagc catggccact ttacccgtcc ctatcgaaat
     2401 aggcgcgtct ttcgtgctat agtagagaaa aatcccttat cc
//
LOCUS       DAWCYS010000271         2434 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2157_length_2434_cov_4.296343, whole genome shotgun sequence.
ACCESSION   DAWCYS010000271 DAWCYS010000000
VERSION     DAWCYS010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2157_length_2434_cov_4.296343"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          617 a          753 c          627 g          437 t
ORIGIN      
        1 aacagcaacc ttctcaagaa tctcccgctt ctataagcgg gagaggttca aaatagacat
       61 aagttatagt tacgaggaga attatcatga aaatcacaca gattatgtat accaatgaag
      121 tggcaggcct gtcgtgccgc ggcgaagaac gctttctgcc ttatggcgaa gccctctgcc
      181 tggcaggccg cgccgtaccg acagcccggc tgtggaaaaa gatgcaggat gacggcgcgg
      241 ccgaattccc agaccagacg ggccacatgg acggcacgtg tcagctgatg cagcagtcct
      301 tcacgccgcg caaagccgtc tacgaactgt atcgcacgga ggccgcacag cagcgtcata
      361 tcgccacgat aacgctggaa cagttcccgc gaccgacagc catcggcggc cacaaagtcc
      421 gcggccatat cgccgtgagc catcccctca caccgtcgag cacagcagaa gccctaagcc
      481 tggcaggcgt cctggacacg gccggtcaaa tcgccctggg cctgggcgtc atccgcctgg
      541 tctatgacga cacgccgccc gtcgccgacg acgatgccgc ccggctgcgc gacgaactgg
      601 cccagaccca ggcctattac cagaccttgc aggaaagtta tgatggctac gtccagaccc
      661 tggaacaatc cctgcgcgaa tgggaaacgc gctgtcccca ggaagacgtc gaaaaaaggg
      721 cccttcccct gcccgacgac tacacgtcgc gcctggaaaa ggccctggaa ggcttccgcg
      781 tctgtctggc cggcggcagc gccctgtggc atgacaaagt ccgcacgcgc ttcccgcact
      841 ggatgatcct ggaaaacaag cacttcgacg tccaaaaact ccagggcgcc gacgtactca
      901 tcatcaacac caaccataca ggccacgccc tggtccgcaa agcctgccag caggccgctt
      961 cccagggcac cctcgtctgc tacaccagcc gctacaacct gaacgctgcc gcagccgaca
     1021 tcctctacgc cttgggcgag taagcctaag agccactgac ttttattttg caacattttt
     1081 gtaaaattga acaaataatt gcatttccca taagattctt tacggcatat tgacgctcgc
     1141 cgcagcggcg cgctatagta aaggcatgag gaggtggtat cgtgaaaaaa tggaaacagg
     1201 ctatcctggc cgccttgttc tgcacgtgta cgacgttgac aggactggct gcagacacgg
     1261 ccaaaacggc acctgccgaa gcggccgttc agacccaggc caccgaaaca ggatctgaaa
     1321 aaacagcaga aaaagccagt gtgcctacgg atccgttagc cggccataca ggtaaaatca
     1381 tcgacaaata ttacctgacc aaaggctcga cctataaaga cgtgacgacg ctgctccaga
     1441 ccatcgattc ccagcggacc gggctggaca caacaaccag gccttattat ttccgcgtcg
     1501 acaaagacct gacggacaag tacttccgcc gtgtccgggc ctatgacgac aaaacgacgg
     1561 aacagattgg cgaatactta gtcgccaaag acaagagtgc cgtctggcgc atggatacgc
     1621 cccacgaagg cttgatttat ggcagcgcta aaaaaatgat gaaaaagagc cgcatcatca
     1681 tctacccgcg ctacctggcc ctgggcagca aaggcatcgt cgccctgcag acgccgggca
     1741 atggcctgac ggccaagagc ctgaatgagt ccgtcgccaa aatcggagac gacaatacca
     1801 tcatccccgt caagacgggc caagtagaca ttctggcaga cgtgaccgtc ggcgacggca
     1861 aagcaacggc atcgcgccgc atctccgtcg tcacccaggc cgacctgcag cgcatggcct
     1921 acaacgccta catgacccag ctttatatgg atccctattg gaattggggg gcgcgcagcc
     1981 ctttttataa tgactggggc tggcatcgcc cgccaccacc gccacgccac ggccatcggc
     2041 cgccaccgcg gagatagaaa aggagctgtc gaacgacagc tccttttaag tggttagcag
     2101 ctagtggcta gtggttagcg gatggttaaa aatttgaaac cagcttctac aaactacaaa
     2161 gcgaacgggg cctcgcatga tggcagaaac cttcgtgcaa cagtcccttc ttctgtatgg
     2221 gcgccacgcg gggcgcccgc accggaatat gtacgatttc ccgacctgtt tgctagaaga
     2281 atcatccaaa tccctaactt taaagagaaa cctctcagac cgcctttggc ggccagctct
     2341 cctattagga gagcctttta tcaagcctca aacacctcac accaaatgtc tgggagcagc
     2401 cgagacggcg ccttccaggc tttctttttc tgtt
//
LOCUS       DAWCYS010000272         2434 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2155_length_2434_cov_7.146280, whole genome shotgun sequence.
ACCESSION   DAWCYS010000272 DAWCYS010000000
VERSION     DAWCYS010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2155_length_2434_cov_7.146280"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          438 a          656 c          750 g          590 t
ORIGIN      
        1 cgtcagcccc gtccgtccag ggacaaactc aacagagacc ggcagtgagc catttcttcc
       61 actccttcat gatgttccct gcctttcttt acaaagggat ttcaaaaact cgtccgtccc
      121 gctgcaggcc tgcggcctga cggttgacat aggcgatgta ccagccgttc caccattccc
      181 cggcagcaca agtcgccgtc tgtccctgcc acttcaaaat gaccaggtga atatctcctt
      241 catggacggc accacagacg acgggcagcg tcccttcttt tttctctttt tccggttgtt
      301 ccttgacttc ttcaggcgca gccgccggaa cggtcagcgg taccgcttca ggcgcagcag
      361 tcttcttcgg cagactgccg gcaaacaaat ccggtaaggg cttatagcgc aaggccggtc
      421 ggacatcata gacgaggttt cctttaccgt ttccccgttc cgcttcgtca gaagcagaaa
      481 aaacgggatg cgacccagct tcatgctgag cggcatcccg tttcatgtgt tcttgtcctt
      541 gcgacggctc gtcccaccac gtccagatgg cgccaaggca gacaagtatc cctatatagg
      601 gaagccatgt cttgcgatgt tgaacgatac cgttccttac cttttgcaga gcggacaaga
      661 ccttattgct tatctccata gccctgcgga tgatgctgat gccagttcca ggcatcggca
      721 ataatttgtt ctaccttact gtgtgtcggc tgccagcctg tgacgcggcg gattttttcg
      781 gatccggcga tgaggatggc cgggtcgccg gcgcggcgcg gtgcttcttc gactttgaag
      841 tctttgcccg tgactttctt ggcggcttcg atcatttcac ggacgctgaa gccgttctga
      901 gagcctaagt tgaagtactg cgattctccc ccttcggcga ggtatttgag gacccttaca
      961 tgggcgtctg cgaggtctgt gacgtggatg tagtcacgga tgcaggtgcc gtcgggcgtc
     1021 ggatagtcgg tgccgaagat cttgatgctg tcccgctggc ccagggccgt ctgcaggatc
     1081 agggggatga ggtgtgtttc cggtgtgtgg tcttcaccaa tggtgccgct tccatcggcc
     1141 ccggcggcat tgaagtagcg cagggccacg tagcgcaggc cataggcccg gctgaactgg
     1201 gccagcatgg tttcgatcat gagcttggtc tggccgtaga cgttggtcgg atggtagggc
     1261 atgtcttcgg tgatcggcgt ctgttccggt tcgccgtaaa cggcggccgt cgacgagaag
     1321 acgaagtatt tgacgccggc ccggcgtaca gcttccagga ggcagtacga gccgacgacg
     1381 ttgttgtcgt aataaattgt cgggttttcc atggattcgc cgacctggct atgggcggcg
     1441 aagtgcatga cggcgtcgat gttcatgtct tccatgatgt gccgtacccg gtcgatgtca
     1501 tggatgtcga cgacgtaaaa agggatttct tccgggacgg ctgcccgatg gccgcgcgaa
     1561 agattatcaa tgacgacgac gttgtggccc tgagccaata aggctttgac cgtatggctg
     1621 ccgatgtagc cggcgccgcc ggtaacgaga atattcattg agagtctgcc tttcgttatt
     1681 tattgagcgg gctccccacg cgggagcccc tacggtatac tgggcgggct ccccatgtgg
     1741 gagcccctac gataatgttt ctgcggatta taaattattt ttcctgtaat tttttcagac
     1801 ggccgccgag gaatttcttg tagacttcga cgccgggctg gttgaaggcg tcgacgttgg
     1861 cgtattcgcc ttcgtaagcg atggacaggc agagcatgaa catcaattcg cccaggtgat
     1921 acggggtcag ggcgggcagg atgtagttgg cgctgggacg gccgtcaccg gcgagggctt
     1981 cggcgttgga gctgcgggcc gcttccagga tctggctcaa aggcaggccg ctgaaggcag
     2041 cgagtttcgg gtattcgtca tagagatggg gtaccgtgac gtcgtcgggc cagttttcga
     2101 cggagacgaa ctggacgact ttgtcctgcg ggccttcctg atgttcctgg gtctgggcgt
     2161 gcatgtccgt cgtgccgacg gcgacgatag gcgtgcggcc gtagttgacg acgttgccct
     2221 gtttgtccag gcgtttgccc agggattcag ccaggagctg gatgtaccat tcggacaggg
     2281 atttcaggtt atccgcatag ggcatgagga cttccaggtc gcggccgtgg ttcttgccgg
     2341 ccaggaattt gaggacactg ttgagcaggg ccgggttctg ccatatatcc cggttctggc
     2401 aggcgtgttc catgtcgcgg gccccgtcga ggaa
//
LOCUS       DAWCYS010000273         2433 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2159_length_2433_cov_5.546678, whole genome shotgun sequence.
ACCESSION   DAWCYS010000273 DAWCYS010000000
VERSION     DAWCYS010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_2433_cov_5.546678"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          613 a          598 c          793 g          429 t
ORIGIN      
        1 agggaggcga cgcccgctgc cgggaacagc aggcagagga tggcatttat gagaaaactg
       61 tggccgagcc agcgcccttc ctgacggcac cgttcgatgg acgcggccag gaggaccgac
      121 aagggcagcc agcagggcag gatgtacgtc ggcagtttcg agtcggaaat actgtagaag
      181 acgtaaatca ggccggccca gagggcgagc agcagcaggt ccctcttatg ccgcagggaa
      241 ccgggcttgc ggaaaatccc gtctttgctg aagaacgtcg ccacgaagcc cgtccagggc
      301 atgaggccgg cgaggaggac cgggacgaag tagtaccagg gatggaagcg ttcgtgcatc
      361 ttggtggcga agcgcagaaa gtgttcgcgg atgaagaaga aatagaagaa gtccggattc
      421 ttctggcaga ccaggtagta ccagggcacg gcgatgacca ggaagaggat gatgcccggc
      481 aggtagaaga gtttgaggaa gaggcgcggc cgcttgctca gcagggcata ccagaagagg
      541 atgccaaagg ggaagacgat ggcgatgagg cctttcgtga gcatgccgaa acccatggca
      601 gcgtagaaga cgagcaggta tttcttgttt tccgtccgtt caaagacgta gaacgagacc
      661 aggcaggcat cgatgaaaaa ggtcagggcc atgtccatga cgccgataga ggcgacgatg
      721 agctgcagtg ccgacgtagc cacgatgacg gcagccagga gccctgtgcg ctggttgtac
      781 atggtcttgg ccagccagaa ggccaggaag acgttaccaa ggcccgtcag ggccggcagg
      841 atgcggccag tgaattcacg gatgccaaag agctccatgg acagggccgt gacccagtat
      901 tggaaaatag gcttctcgaa atagggcatg taatcgaagt ggggcgtgat gtagtcgccg
      961 gacaggacca tttccatggc gacccggtga tagcgtcctt cgtcgaggtc gatgagatga
     1021 tacgacccga tgtgggccag gtacaaaaag gccgcagcgg cgatgaacag gcccatcaga
     1081 aagcgccggt ggcggcgaag tgcgtcagtc ataaaatacc tcctatatat gtgcgttact
     1141 atatagtata caggaagatt ataaataaat cataaaagaa atgacaattt cctgtgtttt
     1201 catatttctg tgacatatgt catttttacc ttaatgcgtc agggacaggc gcggccgtct
     1261 gggtttgacg atgagcagga cgccgcccag gaccaggaga atgcccaggg ctgtatgcca
     1321 ggtgaaggct tcgcccagga cgaggatacc gctgacgacg ccgacgacgg ggacgaccag
     1381 tatggcgacg ccggccttgc cggcttccat gtgggcgagg atataattcc ataagaagaa
     1441 gccgacagct gcagccagga taccgttata gagcagacag gcggcagctg ttgttgtcca
     1501 gtggatggaa ggctgtggga caatcagggc gacgatgaag agcaggagcg agccgacgac
     1561 catctgccag gccgagtact gcaggggcgg actgtcctgg aggtgccgtt tcaggatgat
     1621 gcccgatgcg gcccaggcgg cggccccggc gaggacgatg gagacggctg tcatattgcc
     1681 caggtcatgg atgtccatga ggacgaagag gccggcgatg cagatggcta tgccggctat
     1741 tttccggagc gtcagccttt ctttgaggaa gagctgggcc agaatggcca tccacacggg
     1801 catggtgtag ttcaacacgg agacgacgcc ggctgtcagc gaatacatgc cgatctgcat
     1861 gcagacattg ttgacggcaa tctgcaagat gcccgtagcg gcgatccact tccagtactt
     1921 ccgttccggg acgggaatac ggcgccaggc gcagaccgag agcatgacgg cggcgccgat
     1981 gaggaagcgg aaggccacga acaggacggg cgggaaataa gcattggccg tcttcatgac
     2041 gacccagttg aaaccccaga tggcatataa tagaaataga gcgcgcatca taggaaaaca
     2101 ccttcttttt tagaaattgg ttagcaacct tattttatcg gacgggagag gagaaatcaa
     2161 ggcagcggcc cgtccggcag ggcatggaca gacaggccgg gaacggctat aatggaagaa
     2221 aagatttaca ggaggagcga ttcttatgtt acatattggc ctgaccggcg gcattgcctc
     2281 cggcaaaagt actgtcgtca ccatgctgcg cgaattgggg gcaggtatcg tcgactgcga
     2341 cgtcattgcc cacgacgtcg tcgaaccccg ttcagaagga cttgcggccc tccctgccgc
     2401 atttgggacg aagacgctct tagccgacca atc
//
LOCUS       DAWCYS010000274         2427 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2170_length_2427_cov_4.460371, whole genome shotgun sequence.
ACCESSION   DAWCYS010000274 DAWCYS010000000
VERSION     DAWCYS010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_2427_cov_4.460371"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          654 a          672 c          569 g          532 t
ORIGIN      
        1 aaggaatcgt tggcagagtt tgaaaaggca ggccgcagcg acctcgtcga agaaacgaag
       61 gccgaaatgg ccgtactgga aaagtacatg cccaaacagc tcacagccga tgaaatccac
      121 cagatcgtca acgatgccgt tgccggtcag agcggtctca agatgggcga cgtcatgaag
      181 ctcgtcatgc ccaaggtcaa aggtaaggcc gacggcaagc ttgtttcaca gattgttcga
      241 gaaattttac aaaataatta gttttgttag atttttctaa aaaatctatt gacaaataga
      301 ctgattgatt gtattatttt agcatgattt gaaaataaaa taggtcatga aacaagagtc
      361 aaagaagact ccgaacctta accacagggg cggagtcttt tttttcaccc aaaggaggag
      421 tcatgatggc taaagatgat ttagtatatg taatccctgc aggtcaatat ggtaaagaag
      481 gtgtgctggc tttactcgaa cagcacccgg aaattaaatt tgtatctctc gtaggcatcg
      541 acctcgccgg taacgatacg gacgaaaaga ttcctatcag tgtattcttc gatgaatacg
      601 ataaattctt caatgctacg gctttccaga cagacggttc ttccgttgtc ctgccgggca
      661 tcgctacctt gtccaatgct cgtgtcgaca tcaaagccga ccccggcgtc gactggttcg
      721 tcgattacaa tgacggcaat atcgattcgg aaacgggcaa acccgtcggt acgctccgta
      781 tcccgagctt cctccgccat gctacgggct ttatcgactc ccgttccatc cttcgcaaca
      841 cctgcgacta cgtaggcgac cagattttgg ctcttgtcaa gaaatacggc atcaaaggca
      901 cgtccatcaa tcctgatgac atcgaaaaga tcgtcttcac gacggctaca gaattggaat
      961 tctgggtcaa gaccccgagc caggacgtag ctacccgact cttgtcggca tcccagaaga
     1021 tgcaggaaca gtactggcag cgtacacacg gcgtcgtccg cacggcgctt gaacagaccg
     1081 tcgaacgcct tgaaaaatac ggcctcgaac cggaaatggg ccataaagaa gtcggcggcg
     1141 tcaaagccca gatcgacgat tccggtaaat tgaccttcgt cctcgaacag ctcgaagtcg
     1201 actggaaata cgccagcgac cctgtacaga cagccgataa cgaaatcgct gcccgtatca
     1261 tcgtccgcga agtcttccgc gaaaacggcc tcgaagtcac cttccaggca aaaccgatcc
     1321 acggtgttgc cggctccggt gaacatacgc atctcggcat cggcgctatc atgaagaatg
     1381 gcaaattcgt caacctcttc aacccggatg acatgaagag cgaattcctc tcggccgtcg
     1441 gctatggcgc tatcatgggt atcctcaaac actacgaagt cataaacccc ttcgtatcgt
     1501 cgacgacgga ctccctcaac cgcttgaaac cgggcttcga agctcctgtc tgcatcgtta
     1561 cggctctcgg cggcgaaaca ccggacgttc cgactcgtaa ccgttccgtc ctggccggcc
     1621 tcatccgtga catcaccagc ccggcagcta cgcgtttcga actccgcgct cccaacccgt
     1681 tcaccaatac gtacatcgcc atcgctatgt gctacctcgg cgctctggac ggcatcaaat
     1741 acgctctctc cagcggcaaa tcgacggctg aactcctggc tgaactctcc aagaaaccgg
     1801 gcgaagacgc agattacctg gaaaaagacc gggcataccg ctccgaagtc gacgtcttcg
     1861 aaaacttcag cgacgaagaa cgcaatgcca tgttcggcaa agctccgcgt acggtatggg
     1921 aaaacttcaa agcccttacg acgtatccgg aaaaactgga cgacctcgct tgcgacgctt
     1981 tccagaaacg catcatggaa tccttcaaac tcggcgctct cagccgctgg gcactggaac
     2041 tccagtaccg catcattccg gaaaacctct cgaaagtcat cgctgccgaa cagctccatg
     2101 ctgatggcga cagcaatgct ctcgaagtcg aacgctggac caagattaac gctctccgca
     2161 cggaattagg ccgcgacacc gaaaagagcc tgtccatctt ctccaagatc aaacaggccc
     2221 tggctgctga cgactacgat accgcatcgg acctcatggt cgaaatgagc gacaaaatgg
     2281 atcagctcac cgacatgtac tacgaatatt ctcataacgc tttctaatat atcgtttgga
     2341 tgtaaaaagg agctgtcgca ttgcgacagc tcctttttga gttttaagtt tgtagttttt
     2401 agtttgcagc aaatggtaaa aaattta
//
LOCUS       DAWCYS010000275         2405 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2190_length_2405_cov_5.866809, whole genome shotgun sequence.
ACCESSION   DAWCYS010000275 DAWCYS010000000
VERSION     DAWCYS010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_2405_cov_5.866809"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          583 a          735 c          642 g          445 t
ORIGIN      
        1 gaaccggata cgattgtgaa ggtcgaaaag cgcaagcgca aacgccagcc ccagccgccg
       61 ttcacgacgt cgacgatgca gcaggaatgt gtcagcaagc tcaatttcgg cgccaagaag
      121 acgatgatgc tggcccagca gctctatgaa ggcctcgata tgggcgacca gggccatgtc
      181 ggcctcatta cctacatgcg tacggactcg gtccgcatca acgatgatat ggtccaggcc
      241 gcgcgggagt tcattacgtc gaactacggc gaagaataca tccccgacaa gccccgcgtc
      301 tacaagacga aacagaccag tcaggatgcg catgaagcca tccgtccgac gtcgctggaa
      361 ctgacaccgt acaaggtgtc ggaattcctg tcccgcgatc agctgcgcct gtatacgctc
      421 atttggaacc gcttcctggc cagccagatg gaatcggtcg aaacggaaca catggccatc
      481 atcatcgaca gcggccgcta cgaactgcgg gctgcaggct acaaagtcct gttcaagggc
      541 tttacggaac tctacgaaga cgccaagaag gacaagactc tggctgagct gccccatatt
      601 tcggcccata ccgtcgtcca caaccaggcc gtcacgccgc agcagcattt cacccagccg
      661 ccggcacgct ataccgaagc cagcctgatc aagacgctgg aagaaaaggg tatcggccgg
      721 ccgagtacgt atgcgcccat catggatacc atccagaacc gcaattacgt cgaaaagaag
      781 gataaacagt tcgtcccgac ggaactgggc gtcatcatcg tcgatttgct gaagaaatat
      841 ttcgcccaga tcatcaacgt cggcttcacg gcccacatgg aagaagagct ggacgccatc
      901 gaacagggca aggacacgta ccgccacgtc ctccaggaat tctatgacgt cttcaagccg
      961 gaaatggacg aagctgaaga aaagatggaa aaagtcacca tttccggcca ggattcgggt
     1021 caggtctgcg agctctgcgg cgcgccgatg gtctataaat tcggccgctt cggcaaattc
     1081 ctggcctgct ccaatttccc ggaatgccgc aataccaagg ccatcgtcga agacctgggg
     1141 attacctgtc ccaagtgcgg caaggggacg ctcatcaagc gtaagtccaa gcggggccgc
     1201 gtcttttacg gctgcagcca gtatccggaa tgtgacttcg tcctctggaa ccagcctgtc
     1261 gacaagaagt gtcccctttg tggcagcatc atgattgtca aacattataa aaaaggaccc
     1321 gataagattt tctgtagcaa tgcagagtgt gaaaaccata aaaaaggtga ggtagtgcat
     1381 gaagaatagt gtcttaatca tcggagccgg cctggccgga tcggaagctg cctggcagct
     1441 cgtccgccgc ggcatcccgg tagaactcgt agaaatgcgg ccggtcaaat cgtcgccggc
     1501 tcaccaaacg gaatatttcg cagaactcgt ctgcagcaat tccctgcggg cagcgaatat
     1561 tgaaaacgcc gtcggcctct tgaaggaaga aatgcgccgt ctcggctcgc tcatcatgga
     1621 tgccgccgat acgcaccgcg ttccggctgg cggggccctg gccgttgacc gcgtcccctt
     1681 cagccagtac gtgacggaaa aactcaagga tcatcccctg gtcaccatcc gccacgaaga
     1741 agtgacggaa ttgccggacg accagatctg catcgtcgcc agaggccccc tgaccagtga
     1801 cgccttcgcc gataacatcc gccgcatgac cggggaggat tccttccatt tccacgacgc
     1861 cgccgcgcct atcgtcgccg tcgactccct ggatatgact aaagtctacc gcgccgcccg
     1921 ctacggcaag ggtggggcag actatctcaa ctgccctttc accaaagaag aatacgaagc
     1981 tttctggcag gccctgacga cggctgaatg tgccgaactc cacgactttg aaaaagacga
     2041 cagcgtcttt gaaggctgca tgcccatcga agtcatggcc ggccgcggca tcgataccat
     2101 gcgctacggc cccatgaagc ctgtcggcct ggaactgccc ggtacaggcg aactgcctta
     2161 tgccgtcgtc cagctccgcc aggataacgg cagcgccacg ctgtacaata tcgtcggttt
     2221 ccagacccat ctcaagttcc ccgaacagaa gcgggtcttt tccatgattc ccggcctgga
     2281 acacgccgaa ttcgtccgct atggcgtcat gcaccgcaat tcctatatca attcgccgga
     2341 actgctcctg ccgacgctgc agctgcgcaa acagccgaac ctgctcttgg ccggccagct
     2401 gaccg
//
LOCUS       DAWCYS010000276         2378 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2212_length_2378_cov_5.582006, whole genome shotgun sequence.
ACCESSION   DAWCYS010000276 DAWCYS010000000
VERSION     DAWCYS010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_2378_cov_5.582006"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          503 a          724 c          601 g          550 t
ORIGIN      
        1 ataataggca agaaagaact gctctacgtc atttatagta agcagaccca gaacctggtc
       61 aatgccaaag tcgattacgg cgctgacgtc gaatcggcga ttgccaacct gaccgatgcc
      121 atcgaaaaaa tgggcatcat caaataccca gtccgctggc tggccgtcaa gctcctggaa
      181 aacgactccg acgccatcgc caaagtccgc gctatggaag ggacccagtc catcctggcc
      241 ctggcttcga ctctgcgcga ttccctggcc aacaagatcg atttggactt ctatttcgct
      301 caatgccgat atcaattcgc aactgatgta tttaataaat cgattatcaa tgtcggttcg
      361 agcgactcct tgtccgacaa gatcgaccgc gtcctgaccc accgttactt agggatcccg
      421 attttcctgg ccctcatgtg gctcatgttc gtcgtcgtca tcaacgttgg cgcctacccg
      481 cagggctggc tcgacacggg cttctcgatg ctcggtgact ggtgcagcga tgtcatcgaa
      541 gatgaacagc tccgctccct cgccgtcgac ggcgtcatcg gcggtgtcgg ttccgtcctt
      601 tctttcgttc cccttatcgt cctcttgtac ttattcatca gccttctcga agatacgggt
      661 tatatggccc gtgccgcctt cctcatcgac cgcgctatgc gtgccctggg gctgcacggc
      721 aagtccttca ttcctatgat cctcggcttt ggctgcaacg taccgggcat catggccgct
      781 cgtacactgg acaatgaaaa agaccgtctg gtcacgattc tggcctgccc gttcatgagc
      841 tgcggtgccc gcctgccggt atacaccttg ctcatcgccg ctttcttcgg cgcttccggc
      901 cacggtggta cggtcctctt cggcgtatac cttttaggta tcatcatttc cgtctgcgtc
      961 gccctggtcc tccgtcatac gacctttaag ggcgaacagg aaccgttcgt catggaaatg
     1021 ccgccgtatc acattccgac cttgaaaggc gtcctcatgc acatgtggga acgcacggtc
     1081 ttgtacctca agaaagccgg tactttcatc ctcggcgcat ccgtcctcgt ctggttcctc
     1141 acggcatacc cgatggacgt cgaatattcc caggacttcg atgccgctaa aaatcaggtc
     1201 acggctgaaa tggaacagaa acagtctgac atccttcagt cctacggcct cagcgctatc
     1261 gaagacaatg ccgctttgaa cgatatgtat gaatccatgg tcgctgccgc tgatgaagcc
     1321 gctgacgaag ccgatgctga cgaagccgat acgggcaatg ccctgaaccc ggctgccgct
     1381 ttgggctccg ttgaagacca ggtcaagaat tccgaagaag aagaagacaa cttgttcatg
     1441 ggtaaatacc cccagagctt cgccgacctg caggaacaga atccggccgt cttcgcccag
     1501 gctctgccgc tcttcgatgc caaagccgat gctgacgacg aaacgtccaa actcgacgac
     1561 cagcagacgg ctgaaaaact ggaacagtcc tatgctgccc gcctggggca cttcgtagaa
     1621 ccggtcatcg caccgcttgg ctttgactgg aagatcggcg taggcctcat cgcctgcacg
     1681 gctgctaaag aagtcatggt ttcgaccttg ggtacgattt acagtgtcgg cggtgacgac
     1741 acccacgaat cgggcatcgt cgcttacctc cgtgatgacc cggacttcaa cccagccgtc
     1801 gccctgtcgc tcatggtctt cgtactgctc tacatgccgt gcgtcgctgc catggccgtc
     1861 atcaaacgtg aaacaggctc gtggaaaatg ctccttgcca gcgatgccat gtgcctcgta
     1921 ctctcgtacg tcctggcctt tgtcacctat cacctggccc tcatggccgg cctcggcgct
     1981 taagacttga agcagcccct tccataacgt ataatattag tggctcgtgg ctagcctccc
     2041 ctcataggcc ctttttggcc cgtcaggcgt tgaccgcagg gaaaggcagg acggctgcca
     2101 aaacggagag ccggctggag gtggcccaaa gggccggaga ggttgatttt acaacgaata
     2161 attgctaatc ccgagaggag gtatatctat gagtcctgca accattatcg tcgctatcgt
     2221 cgtcatcatc gccgtagcgt acttcattca ccatatccgc ggcctgttca acggaacggc
     2281 cagctgctgc ggcaactgct caggcagctg ctcatccact ggtatatcaa aagcgggggc
     2341 cgattccaaa gtgcctccgt tctgcaggat atagcata
//
LOCUS       DAWCYS010000277         2373 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2218_length_2373_cov_9.038827, whole genome shotgun sequence.
ACCESSION   DAWCYS010000277 DAWCYS010000000
VERSION     DAWCYS010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_2373_cov_9.038827"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          557 a          697 c          517 g          602 t
ORIGIN      
        1 gatggcatcg agccctcaga aaaacctgca attcatctca ccgcctaaag aggcgggaga
       61 cttcttgcag aatcaggtta aagatgcccc ttcattcaga gcatacgtag cggccatatg
      121 acgcaaagag tgaacaccaa tatggggtat tcctatcttt ttgcacattt ttgtcagcca
      181 tgtattcaga taactaggac ttgcaggaac tagatgatca tgtagttatc aggtaaatca
      241 tctattgcct tcagcaattt cttcagctgt tcttcctgaa taatgggata gtgatgataa
      301 ctccatccct tggatttcgc ccccgatgac aaaggctcct tcgccgtcgg attcggctcc
      361 tacaagagcg aacatgccgc agccctcggc gcctctaaat aacacgattt cctccttaga
      421 caaaaaggca tcctttgact tgcagcagcg agggatgtct ttttgctatt gtttctgaaa
      481 tcttatggtc aacaggaata acacatgaaa aacctttatt gacaggagta tgaaaaggga
      541 ttatactgag ctgtatttag caaaatgcag tttcttacaa taaaaaaggg aggctatcat
      601 gctattcaaa acgattaatc gccaccttct cggcaagcct ttggagaata ccaaactcga
      661 gggcgaacag atgccaaaat ggaaggccct gcctattttc tcatcggatg ccctgtcatc
      721 cgtcgggtac gggccggaac agattgccat cgtcctggct tcggtaccaa ccttagcgac
      781 ataccgttat ttcggaccta tcgtcttagc tatcattgcc ttattattca tcgttgccct
      841 gtcctatacc caggtcgtcc gggtcaatcc gggcggcggc ggttcctatg gcatcgccat
      901 gaaatacctc ggtgtctacc cagcccttac ggcaggggcg gcgcttctgg ccgattacat
      961 cctgaccgtt gccgtttcca tttcttcagg gacggcggcg ctggtcagtg cctttccggt
     1021 cctccaccct tatcacgtct accttgacct cggcgttctc atcctgatga tgatcgtcaa
     1081 cctgcgcggt accagggagg cctcaaccgt cttcgtctgg ccgacgtact tcttcttggc
     1141 tgccatgggg atcaccttaa tcggcggctt ctatcaaatc tggaacggca ccctcacggc
     1201 tcataccgtc gctgtcgaaa cggtagcccc attgaatcag accgccttga tgctcatcgt
     1261 cctgcgggcc tttgctaacg gctgtagttc catgaccggc atcgaagcca ttgccaacgg
     1321 cgtcaccatg ttcaaggcgc ctcagcaaaa aaacgccatc gaaacgacgg ctatcatggc
     1381 ctgtatcctg gcaatcatgc tcggcggcct cggttacctc atcatttatc ttcacctctt
     1441 gccgacacaa ggctacaccc tattgtccct ccttgtcgaa gatatcttca gccggacctt
     1501 gatttactac gtcatccaga ttttgatgat ggtcatcctc tacatcgccg ccaatacggc
     1561 ctacaacggc ctgccgccgc ttctttcttt catggccgtc gacggctacg tcccccgcta
     1621 ctttgccaac cgcggtgaac gcctgagcta ttctaacggc atccaagtcc taacgctcat
     1681 ggccggcatc ttgattgtcc tcttccaggg taatattgag cacctcattt ccctctatgc
     1741 catcggcgta ttcctctcct tcaccattgc ccaatcggcc atggtcgtct actggagaca
     1801 atgccgggaa cgaaactggc gcctcttttc cgtcatcaac ggcttcggtg ccctggtcac
     1861 ggcctgcgtc gtcgtcatcg tcctcatcac caaattcatc tacggagcct ggatcatcgt
     1921 cctgctcatc ccgaccttgg tctatatgtt ccttaaagtc catcatcact atgatgacgt
     1981 ccgagaacag ctgttgctga caccggaaga gtatacgaaa gtcatgacca ccgacttagg
     2041 ccgcaatatc atcatcatcc ccatcgcgtc cccgactcaa gccgtagcca aggccattcg
     2101 ctacgccaag atcatcagcg gttcgaaaga taaaatctat gccgtccacg tctacagcga
     2161 cgatgaacgg ggtaaaaaaa tcaaaacctt gtggcgccaa ctggaacccg acatcgagct
     2221 catcatgctt caatcgcctt atcggcagtt gacggacccg ctcatcacct ttatccaaga
     2281 gctgcggaaa gagacggctc cgaaagatat gattaccggc ctcattccgg aatttgaaac
     2341 gaaaaaatta tggcaccgcc tgcttcacaa cca
//
LOCUS       DAWCYS010000278         2370 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2221_length_2370_cov_9.434557, whole genome shotgun sequence.
ACCESSION   DAWCYS010000278 DAWCYS010000000
VERSION     DAWCYS010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_2370_cov_9.434557"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          592 a          642 c          555 g          581 t
ORIGIN      
        1 atggtcggtg aaaacgtcgc ccatcgtccg ggcggtgccg tttccctggc tgtcggcatg
       61 gctttcatct tctccaacat ccctggcctc gaccacctca tgaactactg gtatcacttc
      121 tgtatcatgt ttgaagccct cttcatcatg accatcatcg atgccggtac ccgtgtcggc
      181 cgttacatgc ttcaggaact cgtcggccgc gtatggccga agttcggcga tccccactgg
      241 ctgcccggcg ctatcatcgc ctcggctctg atctccggtg catggggtta catcgtcctc
      301 caagggaact tgtccaccat ctggcctatc ttcggtgtat ccaaccagct gctggccatc
      361 attaccctgg ccatctcgac ggttgtcatc tgcgctatgg gtaaggcccg cttcgcctgg
      421 gtcacgatta tcccctgggc tttcctgacc gtcgtcatct tctgggccga ttacctcaac
      481 atgttcaaca tctatctgcc caaacaagaa tggctcctgt tcaccgtttc ggccatcatg
      541 gctctcctgg tcatcatcgt cagcatctcc tcgatcagaa gatgcctgta cctggctaag
      601 accgtaccgc ccagctacga tacgacagaa actgtagaag ccgaagaatt gaaacacctg
      661 gaagaactca tcaagaccga cccggtcgcc aaacgcttca aagaacttac agttgatcat
      721 cattagaaaa aagaggctgt cgcattaaga cagcctcttt ttgaatttta agtttgtagt
      781 ttttagtttg cagcaaacgg taaaaaattt aagccaaact tctacaaact acaaactata
      841 aagtaaaagg tgcctcgcat gatggtaaaa accttcgtgc gacagtccct ttttaggtgg
      901 ttcgtggggc gtccgctcgc gcaaaccgac ctcggtattc ccacatatat cgggcgggct
      961 cgccacgtgc gagcccctac aatctataaa cgttttgcct aatcatttaa cgtttaacct
     1021 tgttttattt taaaaattta tggaaaaaca tcttgacata gaccagcaaa ttgcgtacaa
     1081 tgtgttcaag cttcaggaaa caaaccgtga agcgaaatat tcgtaaatct aatgacgaga
     1141 gaaagtagtc tgtaccattg cttttcagag agctggcggc cggtgcgagc cagtacgatg
     1201 atactgatga agctcctctc cgaagatcga cgctgaattt cattaggcgc cgacggcatt
     1261 tcaccgttac cagaaacgcg tatgttggta cgttgtagag agtccggctg cagcaatgcg
     1321 ccgggaatca gggtggtatc gcggaattat actccgtccc ttacagaagg ggcggagttt
     1381 ttttgttggc ctcttttgac aaatctctaa atcccaacga taaaattctt attttatatt
     1441 acaaaggaga tatgttatca tggcaaaagt attttatgat caggatgtaa actgggatgt
     1501 aatgaaaggc aaaaaagtcg caatcatcgg ttatggcagc cagggccacg ctcatgcgtt
     1561 gaacctcaaa gacagcggca tcgacgtcgt cgtcggcttg tatgaaggca gcaaatccat
     1621 cgccaaagcc caggcagccg gccttgaagt taaatccgtt cctgaagccg ttaaagaagc
     1681 cgatatcacg atgatcttga ttccggacga aaaacaggct gacgtctacg caaaagaaat
     1741 cgctcccaac ttgaaagacg gcagcgccct cgctttcgct cacggtttca acgttcactt
     1801 taaacagatt gttcctcctt cgacagtcga cgtcttcatg gtcgctccga aaggcccggg
     1861 ccacctcgtc cgtcgtactt tcactgaagg cggcggtgtt cctgacgtat tcgctgtcga
     1921 acaggacgct acaggcaaag ccttcgacat cgctttggcc tatgcccgcg gcatcggcgg
     1981 tacccgtgcc ggcgtcatcc agacgacttt ccaggaagaa acggaaacag acctcttcgg
     2041 cgaacaggca gtcctctgcg gcggcatctg ccagttgatc accaacggtt ttgaaaccct
     2101 ttgcgaagct ggttatcagc cggaaatcgc ttactttgaa accttccatg aaatgaagtt
     2161 gatcgtcgac ctcatgtacg aaggcggcat ggctaagatg cgtaaatcca tcagtgatac
     2221 cgctgaatac ggcgattaca cggctggccc gcgtgtcgta acccaggaat cgaagaaagc
     2281 catgaaagaa gtcctcaacg acatccagtc cggtgccttc gccaaagatt ggctcctcga
     2341 aaacaaagcc ggcggccgcg ctcacttcct
//
LOCUS       DAWCYS010000279         2362 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2226_length_2362_cov_3.706545, whole genome shotgun sequence.
ACCESSION   DAWCYS010000279 DAWCYS010000000
VERSION     DAWCYS010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2226_length_2362_cov_3.706545"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          598 a          557 c          694 g          513 t
ORIGIN      
        1 aattgtcaag gtcttatctt ttagtgtgta agcaacgtca ctagtttttt cataattcat
       61 gtttgtttct ataaaagatt tgacctggac ttcctggagc gcatctttta ccgttagatg
      121 agccatatag atgcttggaa tcgtaattaa taggccgagg ataactaaaa tgagacgctg
      181 tttgcgaaaa tgagcttcag cgacattgcc gtgaggcggg actttcagaa tcttaaatac
      241 caagaacgaa gacaaggcaa tgaaaaaggc attgatgaag aacaggtaca aggcacccag
      301 gaaaaacgtg tggttcccgg aggccagccc atagccggcc gtacagaggg gcggcatcag
      361 tgccgtggcg atggcgacgc cggggatgac attgctcttt tctttccgcg tgttcccgac
      421 cgcaccggca atcccgccgc ataaggcgac gaggacagcc cagattgtcg gggtagtccg
      481 ggccaggatt tcatctgtcg gcgaggtaaa cggcgaaagg agaaaataaa tcgaagctgt
      541 cagcacggcc aggccgacct ggaaggataa tttgatgagg gatttgcgga taaaagggac
      601 gtcatatgtg gccattccgt agcccgtcgc tacgataccg cccatcagcg gggaaatgag
      661 catggcgccg atgatgacgg cgacgctgtt caagttcagg ccgatgcagg cgatcatgat
      721 ggccatgact aagacgatca agttcgtgcc ttcgatggtg gcgtcggata agatgcgctg
      781 attgatgact tccatcgggg ccgagtcgcc tttcaaatcg attaagttat tccagcggtt
      841 catgattttc ctccttgtcc gggatgtact ttatctgtca ggtataagtc gtgtttgccg
      901 gctgccagca tcttgacata tcgttcatat tgacggagga cgtcggtgat caattcatct
      961 ttctgcatat atttgatgct gtatccgcgt cggccgtcgg caaaataaca aatgggttca
     1021 taattttcct cttccgtaat ggtcgggaag gacagggagc cgacgacagt atcggatacg
     1081 tcattggccc ggtagcggat gccataaatg aaattgcgga ggctcccgct ttcgacaatc
     1141 aattcccggc aggccgtctg actatcatca ctgccctgga tgtggacgga aattccattc
     1201 cgcagaaatt cctggcgcag ttcctggaaa gccgggtcga cggtctgttt gaggaactgc
     1261 tggaaatcgt ccaaggtccc gacggatacg atttttttca gccggtcctg ccaatgggag
     1321 ccatcccagt agacggtact ttctgacaat tttctgctga aataggcgtt ctcgatcgtc
     1381 aagccccgga gcaggcagaa caccatcagg atcatgatga cggcaaaggg cagggcgatg
     1441 atgacggtca tcgtctgcag ggcttggagg ccgccggaat agagcagggc gatggacagc
     1501 agggcaagca gggttcccca taagacagtc tgccacttgg gaaacgtatt cttcccttgc
     1561 gaggcgatgc tgttgatgac gaaaatcccc gagtcagccg aagtgataaa gaaaatgacg
     1621 atgaccagca gggcaatcag tgaggtgacc agggccaggg ggaattggtc caggaacagg
     1681 aataagagct tttccgtttc cgcggcggcc tgggtcagga tgccaccggt ctggccgtcg
     1741 acccagatgg ccccgttgcc gaacacggtc atccagaaga tattgaacag ggtaggaacc
     1801 aggaggacgc agacgacaaa ttcgcgtatc gtgcggcctt tggaaatctt agcgatgaag
     1861 agaccgacga agggcgccca ggaaatccac caggcccaat aggtgatggt ccagttcgtg
     1921 aaccattctt ctttggtgcg ttcgtaggcg aaggtgcgga aagacagttc gatgatgtgt
     1981 gtcagatagt agcctacgtt ttctgtgaag gccgacaaga gatataccgt cgggccggcg
     2041 aagaggatga acaggagcag ggcgatggcc aggatcagat ttccctggct catgtaacgg
     2101 atccccttac ttacgccgga aatggccgac aagatggcca ggccgacggt gatgacaatc
     2161 aagaggacga tgtgggaaaa agaggctgtc gaaaaaatgc cgacagcact caggccggcg
     2221 ttgagctgca tggctccgaa gcccagcgtc gtcgtcagac cgaaaatcgt cgcacagagg
     2281 gcgacgatat cgatcaggtt gccccagaaa ccgttcagtc tatgcttcag gaggggataa
     2341 aaaccggaac gcagggttac gg
//
LOCUS       DAWCYS010000280         2356 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2234_length_2356_cov_4.702738, whole genome shotgun sequence.
ACCESSION   DAWCYS010000280 DAWCYS010000000
VERSION     DAWCYS010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_2356_cov_4.702738"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          498 a          599 c          624 g          635 t
ORIGIN      
        1 acgatgaaag gtgcacgtat gcgcaactgc catcgtttaa aactgactta ctacctgccg
       61 taaacccctc tttcgacacg ttaatagctt ctacatcttg atacatattg atattagtat
      121 acccaacggc cgaagtttgt agccttatag ggaataaaaa aacggccggg gtaacatccc
      181 acgaattggc aaacttgaca gtttgaccat cattcacggt tcctgtgcaa aatctcccaa
      241 tacccgcaaa cgcatgacca ttgctgtcaa taaaggacat gccactttga ccaaattgga
      301 cagagctacc gcttgtttct ttaactgtca taccattttc atccagggta acagctccgc
      361 cgacaatctt taaggcgcca gccatattga tttcattagc cgctaattta tcagcggtga
      421 tagctcctgc tccgatagat tcagcagtaa cagcaccagc agctatccgg tcagtcgtga
      481 tagccccggc tttgattttt tctgtagtca cagagttgga ggccagctcg ttagcggtaa
      541 cactattcgc ggagagttta gctgtactga tagctccgtc tgcaatctta gtgcctacga
      601 cttgagaatc tcctatgtaa gaggagacga taaccccgtt gtcgaatacg gtatccccgg
      661 taatgtgtat cttgctaccg tttatcgaaa taccttcttt agataggttg atttgctgga
      721 taagttcgcc ggtttttact ctcatagcaa tatcgttctg gttctgtaca atagctgtgt
      781 agttactatt tacctcagtt ttcgtatctg ccaaatcttt ttgggtagca gtaaggtctg
      841 tgactaccga agaaatgcta tttgcatttt gcgtgatttg agaaacgaag gaagtattct
      901 tagcgtcctg agctgttttg ttgctctgta ctgtcgacga caagctggta gcgtcctgtt
      961 tgatctggct gatttgggta tccaccgacg agttgacctc gtcctgggac gctttcacgg
     1021 tcgcgaaagt cgaggacagc tccgtttcgg atttgttcaa atcgctgata gacttataga
     1081 cactgcttcc cgggtccaga ttcaagttat tgacgatgcc gttcacttcg ctgtcgagcg
     1141 atgtgatgcg cggaatcgcc atgttggcgt tattgacagc cgtcttgatg tcctggtcga
     1201 cgtttttcag tgctacggcg ttatcggcca ggaactgctg gtcgaccttg tccgtgacgt
     1261 agacgtggag cgtgatgctc ttttcgcctt cgccgaagag gtcatagtag gcggcggcca
     1321 catcgtaaat cccggccgtc ttcacggcat ggttaatagt cgtgttctta gtcttttcca
     1381 gctggtcgtt gatgtagtaa tcgacgccca gacacccggc ggggatggcc ccaacctgaa
     1441 gacgcaagtt gccgatgccg gcctgcgcgt cgatagtcgg cgcagctggc atatttttgt
     1501 tgtactggag cacggccggg cggctgtatt tgtggtccgt actgcacgca aagacacaga
     1561 ccgtgcccgt acggctcttg agaggaattg tcgtgctgag ccggttcgtg cgggccagaa
     1621 ggcctgcatc gtcggcgccg gcactcgtat cggtacggat ttcgtagtat ttgatgtcgc
     1681 agttcgtaac agagtcccag ctgatatgcg aggcctcatc gaagatcagg ctgacgttgt
     1741 caggcgtatt cggtatgagc ttcttcggct cgacatagat ttccgtgtaa ggcgcgctgt
     1801 tggcgtcggt gtacaagccc aattcgtcgg ccgtgcatac agcgattttg tagtattcgc
     1861 cggctatcgc aggcgatata gtcagctggt tcgccccggc cccagcaaag gtccagtcgg
     1921 aagaataccc cgtcttctca gcggccacgc cgttctttaa gaccgcgttc tgagtctgcg
     1981 tcccggtgcg cttataatag acctggccag ccagcttatc cggcctccag gtaatcgtga
     2041 tggaatagtg aggcgtccca tcggacagga acgcatactt ttcgatagct gtcaggtccg
     2101 tcggcgtcac agcgggctct acatggccgg tgcactgcac ggtcgcggcc acgggcttcg
     2161 actcgatgcc gtcgataacg gagcagacct tcacgtgata cgtgagcggc ggcgtgacgt
     2221 tctcgatagt cgcgcaggtg ccgaacacgg tgttgacgcg ggtccagttt tcaccgtccc
     2281 aggagtagta gacgtggaag tcatcgtact tcgcgttctt cggcatgccc caggatgcat
     2341 agatgcggct ggtctt
//
LOCUS       DAWCYS010000281         2355 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2236_length_2355_cov_4.794348, whole genome shotgun sequence.
ACCESSION   DAWCYS010000281 DAWCYS010000000
VERSION     DAWCYS010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2236_length_2355_cov_4.794348"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          524 a          657 c          654 g          520 t
ORIGIN      
        1 aagtcccacc gtctggccgt ctctatggaa gtggaagacg aaatgcgccg ggcttcgcgg
       61 gcggcgacgg acgccaaagt cggcgactgg gtcgaagggg cggtctgcaa catgaccgac
      121 cagggggcat acctcatgac ccgcgaacgc tggatcgcct tcctgcaccg cagcgaattc
      181 aacgggccca tcctcatcgg ccagatgatc aaaggccgca tcacctacct gcgcgaagac
      241 ggccgcatta acatctccct gcgccagacc aaggaaaatg ccatcaatcc cgatgccgaa
      301 cgtatcctgg acttcctcaa gcagcgcaag ggcaagatgc cctatggcga caagacggca
      361 ccggccgtca tcaaagccaa attcggcctg agcaaagccg ccttcaagcg cgccttgggc
      421 cacctcatga aagaaaagaa aatccaccag gaccagggct ggacctattt ggatatatag
      481 gggctcgcgg gtggcgagcc cgcatgtggc acgttgtggg aatcccgcaa gctggtgtga
      541 ttgagcggtt cgtataacca acgggaccgt gcacgaagac atatgtcgtc atgcacggtc
      601 ccgttttgat tttatagggg cttgcacgtg gcaagcccaa tccagaatat gaacgctttt
      661 cccgtttgag gagagtgccg ccaaataccc gacgggctcc ccacgtggga gaccctacgg
      721 attgaacttt ttctgttaga cagaaaaagt tcaatctgtc ccatttacaa tggggtggat
      781 ataccctaaa atagagtaca gattaatcat aaccatacat tggccaatgt gtgaaactct
      841 ataaccgtta gggggatttc acatgtcatc atattctatg gaacacaaac aatcggccac
      901 ttcggctggg agcctttgtt atgcggccgt tttgacggcg ttggtcttcg tcctcacttt
      961 tgtgccgaag attcccattc cactgggcta tgctcatttg ggagacgcga ttatttttgc
     1021 gtcggtcctg ttcagtacac gccggcaggc ggccctggct gcctctatcg ggtcggccct
     1081 gtccgatttc attggcggtt tcccgatttg gatcatcccg acgctgatca tcaagtacgt
     1141 catggtcgaa gccgtctttt atacggtacg cccggacaaa cgggaatgga cgctgacgtc
     1201 gccgcgcgtc ttcatcggtt tcttcttatc cgctgcctgg atggtcctgt cttataccgt
     1261 atcgggcagt ctcctctacg gcagctgggc cgtcggcatc cccatggtgc cgggactcat
     1321 cggcgaaggc gtcgtcaata tgattgccgc ttatgcactg gccgcttgtc tggtcaaggc
     1381 taaattccat ggttatggtt atagcctgaa aagataagga atgattgtat atgcatcaga
     1441 agaaactggc tctggtcaat gatgtgaccg gctatggccg ctgcagcatc gccgtggccc
     1501 tgcccatcgt gtcggccctg aaagtccagg gctgcatttt gccgacagct atcttgtcgg
     1561 tccatacggg cttccacgac tattatctcg acgactatac ggatcacatg acgccgtata
     1621 tcgagagctg gaagaagaac ggcctgacct ttgacggcat cagtacgggc ttcctcggct
     1681 cggcccggca gatcgacatc gtcctcgatt tcctccgcga tttcaaggga ccccatacat
     1741 tggctatcgt cgaccccgtc atgggcgaca atggccagct gtatccgtcg tatacccagg
     1801 agatgtgcga tgaaatgcgg cgcctcctgg cctatgccga cgtagtgacg cccaatgtga
     1861 ccgaagcctg tgagcttttg aacctgccgt accggtcgga ccgcgacttg agcgaggacg
     1921 atttcgccgc catggccaag gccttggccg acaaggggcc gcagggcgtc atcctgacgg
     1981 gcatccacaa tgggccgtac atcggcaatt tcatctatga ccaggggaag atgagctggg
     2041 tccgggccaa aaaggtcggc ggcgaccgca gcggtacagg cgatgtcttt acggccatcg
     2101 tatcggcgtc cgtcgtccgc ggcgaaagcc tggaacaggc cgtacagaag gcgtcggact
     2161 tcatctgccg cgtcatggcc tatacggaag aactggacct gccgcacaac gccggattgg
     2221 cctttgaaga atatctgacc acactgagat gatgtctgcc attcaggcag ctctaaatca
     2281 caatacagcg gatagggttt gatgatcgtt tacactgtag taggacaagg acaatatgta
     2341 gatctcgcag atggc
//
LOCUS       DAWCYS010000282         2348 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2240_length_2348_cov_8.406454, whole genome shotgun sequence.
ACCESSION   DAWCYS010000282 DAWCYS010000000
VERSION     DAWCYS010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2240_length_2348_cov_8.406454"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          496 a          652 c          654 g          546 t
ORIGIN      
        1 ctcttgcacc acgccacccg catccaggcc tccgccgtcc ccttcgacac ggccggccgc
       61 ttctccggca ccgcgacagt cctcttcgaa cagcccgact ggtccgccct gtgggccgcc
      121 aaatggcccc tgctctacgc cggcggcctg tccagcggtg tcgcctacac cctgcagatc
      181 gtcggccaga aatataccga accgacgacg gcgaccctca tcatgagcct ggaatcggtc
      241 ttcgccgccc tggcaggctg gctcatcctg ggagaagtca tgacggccaa agaactgaca
      301 ggctgcgtcc tcgtcttcgc cgccgtcatc ttagcacaga ttcccctgcc acatctctct
      361 ttccgtcaaa agaattaaat tatatttgaa aatattatat ttcaaaatat aatattttga
      421 agtataatat ttttcttcta aattctcaaa aaaaagctgc ctatgtcgat tgacataagc
      481 agcagcgaaa aaaattcatt aaattttgtt gctccgccgc cagatgcgct gggcttcggc
      541 ggctttgatc aagtcttcac gcatatcggg atgggcaatg tttatcaggg cttctgcccg
      601 ctgccacgtg gccaggccgg acaggttggc tacgccgtat tccgtgacca tgtatggcgc
      661 ctgggtccgc ggcgtcgtga tgatatcgcc ggcagtgaac ttggggagga tgttggaatg
      721 gcggactccc tttttatcga cccggctcga cggcatggcc aggaaggctt tgccgtggtc
      781 ggcggcatag gcaccggtca cgaagtccaa ctggccgcca gtcccgctga tgtgccgcgt
      841 accggccgat tcggagcaga cctggccgta aaggtcggcg gcgatacagc tgttgatgga
      901 aatgaaatca tcgagctggc ggatggtttc cggattgttg acgtaatgca taggcgctga
      961 caggacatcc tgattgtggt ccaaaaattc atagagttcc ttcgagccca tacaaatgga
     1021 gaagacgcct ttcccccgct ggaaaggctt tttgtcgttg gtaatcttgc ctgccttata
     1081 caggttgaga tagccgtcgc tcatgagctc tgtatgcatg cctaagtcct tcaagtccga
     1141 ttcggcaatg aggataccca gggcattggg catgccgccg ataccgagct gcagggtaat
     1201 gccgtcgtgc aggtagggga aaatattctc ggcgatctgc ttgtcgatgg cactcggttc
     1261 ccgattcggc gccaggctga ccgcttcctg gctttcgacg atggcctgga ctttcgaaac
     1321 gtggatgtgg tcgctgccca tgccaaagac gacgggcatg ttgggattga cttcgacgat
     1381 gatcgtttcc gccgcgtcga gcatttcctg ggtacagcca ttggtcaggc caaaggaaaa
     1441 attcccgtgc ctgtccatgg gagctacggt gatcatggcg acgttaaccg gcgcaaagcc
     1501 ccgcgtataa taggagccgc agtcgcggaa cagcatcggc tcatggtagg cccggccggc
     1561 atcgagatat ttccggtcga tagcggaaca atgccagaga ttatacgtaa aggtctcgtt
     1621 ttccgggtcg gcttccaaaa tagctacagg gtggcaggaa atggcattgc ggaccttgat
     1681 gtccttcaaa tccccctggc gccgggccag ggccttgtcc agggccgtcg gataagcaca
     1741 gctcatgcca taatcgaccc aatcgccatc cttgacgacc tgcacggcct ggtcagccgt
     1801 acagcatttc cctttatatt cctgatatac gtccatgacc ggtcacctca ctgtctggcc
     1861 gcagccatac cggcgtcaaa ggcttttaag ttgatgtcga caaaggctgg tttcactgtt
     1921 ttggcaatga tgctcttcca atcgatatca tggaggccca gggcttcgat gagggcgccc
     1981 aggaggacga cgttggcact cttgggattg cccaggtcgg tggcgatttt cgtcgcatcg
     2041 aggtcccgga cggggacgtg tttcttcagt tctgcaatga tgccctgggg atactggcag
     2101 gctccgaaaa ggacgggcag ggacgggatc tgcgtcgtat tgacgaccat cttgccgcct
     2161 ttcttgagga agcgggcata gcgggcggct tccatttctt caaaggaaac gaggatatcc
     2221 gctgagcctt cgccgatgat gggcgaatag acttttttgc cgaaacggac ctgcgtgctg
     2281 acgctgccgc cgcaatggct catgaactga acttaggaca acttgacatg taacatatac
     2341 acatctga
//
LOCUS       DAWCYS010000283         2327 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2258_length_2327_cov_5.593310, whole genome shotgun sequence.
ACCESSION   DAWCYS010000283 DAWCYS010000000
VERSION     DAWCYS010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_2327_cov_5.593310"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          595 a          695 c          540 g          497 t
ORIGIN      
        1 gctgttctcg ttgaagcagg aagctcccgc ctctataggc gggagtacgt tcactgtgaa
       61 tcccctgctt ttccgcttcc ttggctacgg cttcgacgac ttcggcatgc ggaccggctg
      121 tggccccgat cttgatttcc ttgtcgctgc tgccggaact ttccattgaa tcatcaatga
      181 taccccctct taagattgtg cctaaatgat acgctttcag tatagttttg tcgatggcct
      241 tcctggcctc ttgacaaatc ccctatccat gataaactaa gaatcaagac aactttagac
      301 gacatttatc cagagtggtg gagggactgg ccctatgaaa cccggcaacc gacgatgacg
      361 gtgctaattc ctgcggatta ttccgagaga taaagaatca gcaatcaacc tctttctctt
      421 tcggaaagag gtctttttta tggataaaat gacggaacag gatagtctat attattctat
      481 attttgttag gaggaatctt catgaaaaat ctcaagaaac tctcgcttct cgccgcttcg
      541 gccctgctgg ccgtcgcttt cctggctgcc ggctgcggca gtgattccgc ttccagcggc
      601 tcgtcttcca agaacacgac ggtcaccgtc ggcgctacgc ccgtaccgca cggcgaaatc
      661 ctcaacgaaa tcaagccgct cctggccaag gaaggcgtcg acttgaaagt cgtcgaattc
      721 acggattacg tcaaaccgaa cctggccctg aacgacaaag aactggacgc caactgcttc
      781 cagcattcgc cgtacctcga taaattcaac agcgaacacg gcacgaaact gacagccatc
      841 gccaagatcc acgtcgaacc gatgggcatt tattcccata agatcaagga cttgaaagac
      901 ctgccggacg gtgcgaaagt cgccatcccc aatgacccga ccaacggcgg ccgcgccctg
      961 ctcatcttgc agagcgccgg cctcatcggc ctgaaagacg gcggtaacat tacctcgacc
     1021 gtacaggacg tcgaaaagaa cgataagcac ctccagttcg tcgaactcga agccgctcag
     1081 attccccgtt ccatcgatga cgtggacatc gctctgatca acaccaactt cgctatggaa
     1141 gccggcctta atccgctgaa agacgccctc ttcctggaac cgaaagattc gccgtacgcc
     1201 aatgtcctcg tcatccgtga aggcgatgaa aaccgtccgg aaatccagaa actcgtcaaa
     1261 gccctccagt ctccggaagt caagaaattc attgaagaca aataccaggg cgcaatcctg
     1321 ccgagcttct aataaaagtt ggatgtttga cgtttgatat tgagggggaa ttcatataca
     1381 tgcatacgaa tgagcttttt gccaaaaaaa cggtcttttc ttttgaagtc tttccaccga
     1441 aacgggacat tcccgtcgaa acggtctatc cgaccctgga cgagctgagc cagctccatc
     1501 ctgacttcat cagcgtcacc tgcggcgccg gcggcacggg cggtaaccgt accgtcgaag
     1561 tcgctacgta catcaagcag ttaggctgcg aaacggcggc ccatatgccc tgcatctacc
     1621 tcacggaaga agcagccatg cagaacctga aagaattgaa agaagcgggc atcgaaaaca
     1681 tcctggccct gcgcggcgac gaagtaccgg gccgcgaacg ggctcacgtc tttgaacatg
     1741 ccagcgacct cgtagccttc atcaaagaaa aggaaccgga cttcaacgtc atcggcgcct
     1801 gctatcccga aggccatacc gaagccaaga ccctggctgc cgacatccgc aacttgaaga
     1861 ccaaagtcga cgccggtgcc agcgagctca tttctcagct attcttcgac aacgcccttt
     1921 tctaccgctt cctcgaacgc tgtgaaatcg ccggcatcaa cgtccccatc gaagccggca
     1981 tcatgcccgt caccaataaa gctcaaatcg aacgcatggt caccatgtgc ggcgccacgc
     2041 tccccatcaa attccagcgg gccattgcaa aatacggtga cactcccgaa tccatgcgcg
     2101 acatcggcat cgcctatgct gtcgaccaga ttgccgacct cctggccaac ggcgtagacg
     2161 gcatccacgt ctacaccatg aataacccgt acatcgccaa aaaaatcgcc gaagccgtaa
     2221 agacattgtt atagaaatct ttgtacatca cctctatagt aggggcttgc acgtggcaag
     2281 cccgtcccag aacattagcg agtctcaagt tcagggaatg cattgcc
//
LOCUS       DAWCYS010000284         2325 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2263_length_2325_cov_3.944934, whole genome shotgun sequence.
ACCESSION   DAWCYS010000284 DAWCYS010000000
VERSION     DAWCYS010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2263_length_2325_cov_3.944934"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          564 a          528 c          547 g          686 t
ORIGIN      
        1 ctttctgtgt tcctggctgt cgcgtgattc attatcgccg tgacacttta catgcacaaa
       61 gaagaaagac gcgtcacaga gccactgaca gtaaaatatg aggattcgac caattccggg
      121 aaaatgaaga aggcgttagg tgtcgatgcc ggcacatctc gagagattac gcgggaaatt
      181 gagcggatcc acgacggcga tgtgcggccc atcgtcactt acaccgtcga ggcgccgacg
      241 gtagaagcgg cggccacgga aacggtgaaa gccatcaaag agggagatgc ccgactaccg
      301 gcccccgcgc tggagcaaac cgaccggact gtcgtcacgc cgaacacaga caaacaaacg
      361 gtcgatgtct acaagataaa cctgcgtaac aatcacaaaa tcaaagccgg actgctgtca
      421 gcggacggca agacctatct cggtgccggc taccaggccg gacgatggga aggcatggta
      481 tacacgagat ccgggaaagg cattgacgcc gcttccgtct cttacacaat caagcagtgg
      541 taagaataag cccctggtca cgagtgtggc cgggggctat atacaaaaag gtagataccg
      601 ggctgatgaa ctcaagaacg aactcaaaaa ggacagctcg aaatgagctg tcctttttag
      661 ctatagcaca tcagctgata atttttaata cacgtaaaag ttttagagta ttcggctaaa
      721 aataactttt tttccgccat tttttccgtc gtcggtggcg aaagcttgta taaaagcata
      781 taaaaacata gttaaatatt tttagctact tgcttgataa tcggcgtaat tacgctagcc
      841 agtttaaaag tatagaaccc tatataaacg tattatttat tactaccgat aatttaacgt
      901 tatcggaagt aatcgagggg ctatttctac cccatgttca aaatgactgc aaaatcgctg
      961 tatcgagttc ttcgcagtca ttgattgaat tcatttattt tatcttggct atttgtgatt
     1021 ctccctgctg atatgctatt atttttttga tgaaaagttt ttctgtgttt tataatatat
     1081 atctgtatct gtttgcagag atatatatat tgttaggctt atgcaggctg gttggaatcg
     1141 gatataggat ccgtggtatt tttgggtaca ctaagcggag tcgacgcctg gattgtgcga
     1201 atcgctatat agtccgcgat attgtcgtat tatcgcatga tttagtatca ggctgatgtg
     1261 ttatattatc tatggatatg ggcctttttt ctatacagta gtgttttaat agttgagcta
     1321 atcaactatt tgcaaaatca gccttttctc gtaaaaatag ttcaaaaaat tatttcttgc
     1381 gaatgatttt ctctggcagt ataattacat ttatctatca atttattttt acggagggtt
     1441 attcgcgatg cagcgatttt tatcttatga aaatacgatt ttagccgtcg tcatgatggt
     1501 atctttcatc aatcctttta ccagcagcgc cctgacgaca gccctgccta gcatcggtac
     1561 ggcttatggt gcgactgatg ctcagctcag ttgggtcatt gagttgttct tgatttcttc
     1621 gaccgtgacc atcatgccaa tcagcaagat tgccgaccgg attggcaagc gtcatatctt
     1681 tttattcggc gtctccttgt tctgtgtctc ttcgctggcc gtttattttg tgaactcctt
     1741 gtacggtctg ttcttgctgc gaatcctgca ggggctgggc agtgccagta ttttcgcgac
     1801 cagcatggcc atcgtatccc tggtcacgcc gccacagcgc cgcggccggg ccatgggctt
     1861 tacgattgcc gccgtatatt gcggcctgtc ctttgggcct gtcctgggcg gcttcttttc
     1921 ttattactta ggctggaaga cgatttttta tttcatcgcc ttcatctgtg ccctggcctg
     1981 gctgattgca gcccgcagca tgacgaagga agaatggatc gtcaacgcga aaggcagtct
     2041 cgatttgcct ggcagtctgt tgtatgccct ggccatgatt gccattatgg ttggcttatc
     2101 ggaagtaatg actctggcat acgccaaata cctcttagct gccggtctcc tcttgttcct
     2161 agctttctta tacggtgaat ggcaccggaa aagcccggtc ctgccagtcc gtatttttgc
     2221 aggcaaccgc gtcttctctt gttccagtct ggcggccatg ctcaattact gcgcgacgtt
     2281 cggcatcagt atcctcttgg acatttactt gcagcgcatt accgg
//
LOCUS       DAWCYS010000285         2313 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2275_length_2313_cov_4.927369, whole genome shotgun sequence.
ACCESSION   DAWCYS010000285 DAWCYS010000000
VERSION     DAWCYS010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_2313_cov_4.927369"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          552 a          680 c          630 g          451 t
ORIGIN      
        1 gccttcgcct aggccccaga ttttccggtc gacggcggtt cgctcggcga aatgcacgct
       61 caaaaaatcg cccagatgcc ggaaatggct ctgaagctgg gagcccctgt catcggcatc
      121 aatgactccg gcggtgcccg catccaggaa ggcgtcgatg ctttgtccgg ttttggccgc
      181 atcttcatga ataatacggc cgcatcgggc gtcatccggc agatcgccgt catcatgggg
      241 ccctgcgccg gcggtgccgt atacagtccg gccctgatgg acttcgtctt catggtcaag
      301 aatacgtcgg atatgttcct cacgggcccg gccgtcatca agtcggtcac ggccgaagaa
      361 gtgacggccg aagaattggg cggcgccatg acgcacagct ctatttccgg cgtggctcac
      421 tttgcctgtg aaaatgaaga cgactgcatc gaaaagatca aggccctcct cgacttcctg
      481 cccagcaaca atatggaaga agcgccggtc gtcgaatcgg acgatgaccc gaatcgcgaa
      541 gacgaaagcc tcaattcggt catccccgac gacagcagca tgccttacga tatgctcgac
      601 gtcatcaaat cctgcgtcga cgacggcaat gtcttccagg tcctggaaaa ctatgccctc
      661 aatatcatca cctgcttcgc ccgctttgac ggccagaccg tcggtatcat cgccaaccag
      721 ccgaagttcc tcgccggctg cctggatatc aacgcatcgg acaaggcggc tcgtttcatc
      781 cgcttctgcg acagcttcaa tatccctgtc gtcaccttcg tcgatgtacc gggcttcctg
      841 cctgggacga ctcaggaata cggcggcatc atccgccacg gtgccaagat gctctatgct
      901 tactgtgaag cgacggtccc caagattacc gtcgtcaccc gcaaggccta cggcggtgcc
      961 tacatcgcca tgtgctgccg ccagctcggc gccgacatgg tcatggcctg gccgtcggca
     1021 gaaatcgccg tcatgggccc cgatggtgca gccaatatca tctttaagca cgacccggaa
     1081 aaagaaaaga agaaacagga atatatcaat gaattttcga cgccctataa agcagccgaa
     1141 cacggccaga tcgattgcgt catcgaaccg aaggaaacgc gtccgcgcat catcagcgcc
     1201 ctggctatgc tttacagcaa gcgggaagaa cggccgtcga agaaacacgg caacatgccg
     1261 ttataaggag gctgctgtat gatagaaatt caatttgccg tcatcgtcct gctggtcgcc
     1321 atgacattgg tcctgagccg gtactataat acccgcttcc gcgctctgga agacaagatg
     1381 aaggacgttg ccgacttcca gcagagcgtc aaggaactgg ccgaagccgt ccaaaatttg
     1441 aaggaccctg tcaaggttga aaaaacggtc ccgtcggaac cggaagaagc ggaaaaatcg
     1501 gaattgacga accccgtcgt ccctgtcggc cagcgccagc ccgccgtcgt cgcagcccgg
     1561 ccgatggctg tcgtaaaaga aacggccccc caaggttctc aagatggcct agacgatgaa
     1621 gtcgtcgctg tcatcatggc cgctgttgcc gcctatggct atccgctgtc ggcaatccgt
     1681 tctatccgtc cgcagaaacg cggttataaa cgccatcggg aaagctgggc tatggcagcc
     1741 cgcctgggtg gtatgagtcg ttaatgaagt gaatatttaa atccaggagg attatatata
     1801 tgaaaaaatt caaggttacc gtcaatggaa aagcatatga agtagaagtc gaagaaatgg
     1861 gaggcgctcc ggcagctccc gcaccgacac cggcagcggc tccggctccg gctgccacgc
     1921 cggctcccgc cgtaggcggc ccgatcccgg aaggtgccat caccgtcaag gcccccatgc
     1981 cgcgcaacat ttcggccctc aaagctgaat ctggcaaagc cgtcaaacga gccgacatca
     2041 tcctcgtcct ggaagccatg aagatgcaga acgacatcac ggcgacggca gacggaacgc
     2101 tccacgaaat ccgcgtcaac cccggcgata acgtaaagac cggcgacgta ctggctgtta
     2161 ttgtaaaata gtggctcgta ggggacgccc aaagggcgtt ccgcaggccg caagccgtaa
     2221 aaaaaagtgg ctgtcgcatg aaggtttttg ccatcatgcg aggccccttt tactttatag
     2281 tttgtagtac ggagtttgta ggtggtagaa gat
//
LOCUS       DAWCYS010000286         2293 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2296_length_2293_cov_4.883825, whole genome shotgun sequence.
ACCESSION   DAWCYS010000286 DAWCYS010000000
VERSION     DAWCYS010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2296_length_2293_cov_4.883825"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          623 c          644 g          467 t
ORIGIN      
        1 atggtccagt gggtaaagcc gggcgacggt tcctgcccga ggcccaggat gagcaatgtc
       61 ccggcaaaga ggatgaccag gaccgtcagg accttgatgc cggcgaagat gaattcgctt
      121 tcgccatagg accgggtcga caagtagttg aggacgaaga ggataatgag gaacaggccg
      181 ctccagatgg cagccggtac gtcagggaac cagtatttca tgatcagggc gccggcgacg
      241 agttcggctg ctaccgtgat ggcccagttg aaccagtagt tccagcccag ggcaaagccc
      301 agggatttat cgacgtagcg gttggcgtag gtttcaaaag aaccgctgac cggcaggtac
      361 gacgccattt cgccgaggcc tgtcatgagg aaataaacca tgataccgat gagggcatag
      421 gcgacgaggg caccgcccgg accggcagag ctgaccgttt caccgccggc gacgaacagg
      481 cccgtgccga tagcgccgcc gatggcgatc atgttcatgt ggcgggcttt caggctgcgg
      541 cgcagtccct gctgctgttg agtttctttc atgcaaaatc acaacctttt tttcattgaa
      601 ttgataaata aaatctaaaa aaactcatac ccttacatta taaaggatga atccttctct
      661 gacaaatgga tattctgcat ggaagtaaat cgtttatgcg attaatggat atataacgtg
      721 acgctgtagt cttgacaagg atttcgagaa caggtacact aaagataaaa tagttgaaca
      781 tgttcaagaa ggtgttttta tggaaaaaga taaagacgaa gtccgtcagc gcatcctggc
      841 tgcggcgaag gagctctttg tccagcaggg gtataaaaag acgacgatcc gtcagatcgt
      901 cgagaaatcg ggcgtcctca cagggagcat ctattatttc ttcaagaata aagaagatat
      961 cttccagtcc ctggtcatgg agctgctgcg gcagtgtacc gatttgattg cagcccattt
     1021 caggcaggag tcgccggcct tccgctacgc cgccctgtgc ctcatcgaat ttgaagccgt
     1081 ggccatgaac cagctcgtcc gcgaaaccta ttatgaaggc tatacgacgc ccttgacgtt
     1141 tgaaaagatc acggaccatc tggccgatat gacgggccgc atcttcggcg attccctggg
     1201 attatcgaag gaggatattt tcctgcgctc cctgctcatc aaaggggcca tgcgcggcta
     1261 catcgtccag ttcgacttca gccagcccat ggacagccaa aagtacgccg ccctggtcct
     1321 ggatacggct ttgcagctgc tgggcatttc ggctgctgag agccatgacg tcctggctca
     1381 gctcgaacag cgcctgccgg aaatcgaagc cattgccgat gagatgatcc accggcgata
     1441 ggacggcttt ctgtaaagga ttgccgcatg aggctatttt ggggcttcat ggggcagcct
     1501 tttttgctca agatttgaac atgttcaaca aacaaaaggg ggaactcatc atgttcatag
     1561 aaaaaaagaa atgggctgcc ggcttgctcc tggccctggc cgttgtctgc ctggccagcg
     1621 gctgcagttt gcagaaggaa gcagcgtcag atgaattgtg gggccgggcc gatgccaagg
     1681 aaatcgacat caactccaaa gtcaccggcc gtgtcgtcca gctccgcgtc aaggaagggg
     1741 atacggtcaa gcagggcgac atcatcgcct acatcgacca gcgcgacctc ctggcccaga
     1801 aggcccaggc cgaagccaat atcaaggccc tccaggccca ggtgcgccag tccggcgtcg
     1861 tcacggacct ccaggacagt acgtccaagt cccaagtcga tacggcttcg gctggcctcg
     1921 attcggccca gtccaatctg gacctggcag aaaaggattt caaccgttac caggaactct
     1981 atgcccaagg agctatttcc aaatccgtct tcgatgccta taagaccaag tacgaagttg
     2041 cccagtcggc gtacagccag gcccagtcca gcgtatcgtc ggcccaggcc ggcctcttgc
     2101 agaccgatgc caacttagcg gctaccgatg cggcccagaa gaaactggaa gcggctcagg
     2161 cccggctgca gcagatcgaa gtatccctgg atgaaacaga actcacggcc ccgttcgccg
     2221 gcgtcattac ggctccagac agcgaagacg ggtccctgca ttccctgggg ccgccgcagg
     2281 tcgcgctgca gga
//
LOCUS       DAWCYS010000287         2290 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2299_length_2290_cov_4.480537, whole genome shotgun sequence.
ACCESSION   DAWCYS010000287 DAWCYS010000000
VERSION     DAWCYS010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_2290_cov_4.480537"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          474 a          639 c          548 g          629 t
ORIGIN      
        1 gaataccaac gcatgctgcc cgttcagcgt ttcaaccaac tccataaacg actccacctt
       61 gccgtcatgg actacatgca cctcgtgctc atcgtcgtag atagcgccgt tggccagctg
      121 ttggagcttg ttcgacagcg ccgccgccga tgtcacgtcg atatcgccat ccggcaaagc
      181 aaggatcatc gttttttcca gctcccgata ggcctttttc gctttcggcg tcagcactac
      241 cggcacatcg tcatagatgc acgccggcag ttgcagatag tccgacgctt tcatgctgat
      301 acagatgtcg gctattttct tcatgacggc atcctgagcc ccgtctttcg ggtcatactc
      361 gtaaatcaca tcacggctgc ggcgccccgg ctcaaagtag cgctcccgaa agtgggtata
      421 atagcggccc agccgttcgc cccggtccag cagatacacc tgactccaca agtccatcaa
      481 gccgttcggc gacggtgtcc ccgtcagccc gacgatgcgc cggatgtgag gacgaatggc
      541 agccagggcc ttgaaccgtt tcgcgctgtg actcttgaaa gatgaaaatt catcgcatac
      601 caccatatca aatggccagt cgttctggta gtaggtgact agccatacga cgttctcgcg
      661 gttgatgaca tagacgtcgg ccggcgtatt cagcgcccgg atccgtttcg tttcgccgcc
      721 caggaccgtg gaaaagcgca agtgcttcaa gttatcccac ttctgcgctt cccgctgcca
      781 cgtcgcctcg gctactttct taggcgcgat gacgagtacg cggcggacca aaaagcggcc
      841 gtatttcagc tcattgaccg ctatcagtgt gatagctgtc ttaccaaggc ccatatcgag
      901 aaaaaggccg atagccggtt tctgcaaaat ctgccggata caatactgct ggtatggatg
      961 cggcttgaat atcatattgc atccctccta atcgttacgg tttccattct acgcccaggc
     1021 ggatggagtg gacgtctaga tactcattga cggccgtttc gccccgcagg acgtaaacga
     1081 cgcagttcat gtgctgcaag cgtttcatct gtgccgtctg tagcggcgtc ggcttattcg
     1141 tttccgtttt cagctcgacg aattcgatct taccgtccgg gaaaatgacg atccggtccg
     1201 gtacgcccgt atttccaggc gatacccact tataggcgat accgcccatg gcttttacgc
     1261 cgctgaccag ctttctttcg atgtttcttt ccagtgccat tgtcaggccc cccttgtata
     1321 aattcaattt tgatactgcc cggctttttt gcgggtgtac ccattctatg taaaaagtat
     1381 ttttttttag aggatttgag gacgcttata cacgtaatgc atacgtctat tttctctaat
     1441 ccccctaata cccctctaat tcttactttt tactatttat atataactta tgggtacatt
     1501 gggtacatat ggtaaataat gggataagta cgcggtttaa gctgtaccca taagcaaatt
     1561 taaagaggta cttatgggta caccttaaat gcgttttagt tcttaaaact ttatcacgcg
     1621 ttctttatgc tttttaagtc ttgctcaaat tcgtttttaa tgggtacact ttgggtacgc
     1681 ctcttttctg tatattaatt agttttatta catttttatg atttcaaacc cccgctgcgc
     1741 gccgcagtag ccatatcggc gcttgtcttt gttgcgcttc catcccggca ggctggccag
     1801 aatgtagtta atgtctcgcg aatcggcccg cttcatattc cggggatccc cgccaaagca
     1861 cgcacaccag atttataagg cacatacttt cgtccgccgt gtcgtcgtgt cgcattgggc
     1921 cgtacccgcc cagtacatcc gccgggccgc caggctcagg ctgtcataat tgtccggtat
     1981 cggcttttct aaaaagtcgc ggatcatgcc ttctttgacg ttgtcctcgc ggtgctgttc
     2041 ctgttcggcc ttggccgcgg cgtcgagttc cggtgtatcc atgtacagcg gctcgccctg
     2101 tttccagtaa tagacggctt ccgcccatag ttggtcgact tccccgggca gttcgctcca
     2161 gatgtttttc gttggtgtgc agataccgac atcgacgggc caaaagcggc ggctccccgt
     2221 cgggtccttt aaaaagtcat ggtcgttgca agtaccaaag aaaatgcact tgcgcgggaa
     2281 gcggcccgtg
//
LOCUS       DAWCYS010000288         2280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2315_length_2280_cov_5.077303, whole genome shotgun sequence.
ACCESSION   DAWCYS010000288 DAWCYS010000000
VERSION     DAWCYS010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_2280_cov_5.077303"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          520 a          553 c          580 g          627 t
ORIGIN      
        1 aaatttaaag ccatccgcta acaactaact gctagccact aacaacttta aaggggcttg
       61 tcatcgtgcg tcaagcccct ttaatccttt atgttcttca atcaataatc cactcgacgt
      121 cgacggattc gctttctgtc gttgaattgg cagccagctg gttagagagg tctaaggatt
      181 tgggcttttc atatcttagc gaatcaggcg ccagcatatc acaataacgg attgaatctt
      241 cgctatagtt attcatagat accgatttga tgccaatgat tttttttcct accgtagctg
      301 cgatggcttc tgcccgatgt ttggaattgg cgacagccta ttccttgctt tttcaaaatt
      361 agtggggaat agaccgcgat tggctcggat tcagatgcgg cattgcgccg cgacaaactc
      421 agactgacaa cgacgcttcg ggaagccctc agcgcgtagc gggccgcccg ccgctgtcat
      481 cccgtccggg gaattgtctc cagcatctga cgggtcagaa cgctctgggc ggcccatacg
      541 gaatcgcagt caggcgatgg gaataacgca gtcacaggtc acaaaaaaac acggtcaatc
      601 actatggtgc cgttcggcca ggggttcaag ggcaacgtgg tgatgcggca ggacccggcg
      661 gggaacatca aaacggacaa agaaaaatcc gtcgaaaaaa tcgacggaat cgtggcgtcc
      721 atcatggcac tggaccgctg cgtccgcaat gggactggca gcggcagtgt ctatgatgaa
      781 cgggacgtta tttcattttg atttttgtca gccggctgca gccacctgga gtttcaggtc
      841 gagggcctgc ataagcttga cgagagtatc gagctttggc gttgtcttca gtgtttcgat
      901 acgggctacc gaggactgtg ggatgccgca tcgctctgca agggcgcgct ggctgatgcc
      961 cagttcctgt ctccgccgga tgatggagga aacaatgtcg cttacttctt cgatttcttc
     1021 catattgcgg cgttcttctt cgctgacggc ctttacatga ttcttatagt cttcccaggt
     1081 tctcattgtt ctgcctcctt ttggcggatg taatcgtcgc gttccgtttt ggcacgggcg
     1141 atttcccggt tcggtgtttt ctgtgatttt ttgcggaaat gatggagcag tacatactgg
     1201 ttctcgtcat agtaaaaata aaagacccgg ttgtttcctg gacgcagttc ccagatgtct
     1261 tcttccagat gtttggtgat attgaccggc agattggtgc cgttttttgc gagcaggtcg
     1321 atgtagaaga gaatctggtt gtactggata cgggcatcct tgctgctttt gcttttcacc
     1381 cgcagggctt ccagaaaatc ccagacttct gattcgccgt gctggttctc ataaaattca
     1441 atcttgtaca tcatgatctc ctttatgttc ctatttagat gatagcataa atgctatcaa
     1501 aaggcaaggg aggcaaagca gcatcgattc attgcatttt ttgatttcat gtcaggaggt
     1561 tttcatgcat atcccttttc cattccatta cagataagtc cttggtttcg tggttctgat
     1621 atccgcaaac atggcacagc tggctgcttg gataccactt gtctatcttg ataagctgtt
     1681 taccttgcca agcaagcttg tactccagca tgttcgtgaa catattccag ccattgtcag
     1741 ttacggattt gccaaagctg aacttgccac cttttttctg ctttgccatc gctttgacgc
     1801 taatgtcctc aatgccaatg gtatcatagc ggtctgcaag gtagcgcgtc ttaaagtgcg
     1861 tttaaaaaca gagagctggc aatttttcac aaaaaggaaa aatgccagct attgtgttag
     1921 ttatgggaaa gaaggcaggc tgcagctgaa tgcgccctcc gcgcgtagcg ggccgcccga
     1981 cgctgttgtc cgttctgata accggtcctg tacctgatgg gttagaaaag ctctgggacg
     2041 gcccctacgg aatcgcagtc aggcgatgga aataaacgca gtcacaggtc ataaaaatac
     2101 acggcccatg gccttgtgcg gcccgctgtg agattcggtc acagcccatt cgagtataat
     2161 ggtaggcttt tacgagccac gaaccacgaa caacggcctt tctctcggat tcagatgcgg
     2221 cattgcgccg cgacagattc atgaaatgaa cgcagttcgc aggtggccgg tcgcagttcg
//
LOCUS       DAWCYS010000289         2278 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2318_length_2278_cov_4.360324, whole genome shotgun sequence.
ACCESSION   DAWCYS010000289 DAWCYS010000000
VERSION     DAWCYS010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2318_length_2278_cov_4.360324"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          647 a          574 c          539 g          518 t
ORIGIN      
        1 tcaattcgag gccatcaacg cagattccca acatgacacg gaccatatat ggacgagtaa
       61 taattccagc tgataacgct gtatgtatag aggccgtatg gacatcagcc atgagcttct
      121 tcccttcatc agcaatatgg agatctgcaa aagcctggtc aaaataaggc tgagaatagc
      181 tttcaccgac aatgaggaaa atatgagatg gctgcttgat tttcgcaccc ttcgcttcat
      241 ggcggaaagc ttccattgcc tggtcaaaag aatcgataca accgccacgg gcctcgacaa
      301 agggtttgac aaggcgtaag ttttcttccg ggctcttttg catcgtctcg ttcataggat
      361 ggcgtcgcag cattttcagt gcgattaaat catcgacagt cgctttcgac aagaaagggt
      421 cttccttgac gacagccggt atcgtatccc attctggctt attgtcatgc atgaaggtac
      481 cgccgtaacg gaagaaatag aaggctgcaa tcaaggccag gacgatggcc gtattgacaa
      541 gataataagc gattcccgta atggccggat aagatacagc cgggatggcc agtatggcct
      601 gcaaggcagc aaagacgaca acaaggtaag gcagtatcag agccagtttc cgggcaccgt
      661 ggtactgatt gaaaaagaca tccatgagat tatgtttctc cgctttttgg ccaagaaaca
      721 caatattatt gaaaatatca tgaaattgag aataaaaaat catcttgcct acgaaagcca
      781 catagaggac aaaggcataa gccaggccga gcagcgtgcg gataacatcg ccgtgctgac
      841 tccaccaggg tatgaacagg cccggcagtg tcaccagaac caaagaaata acgaagacat
      901 aggcattgag atccatgccc caccaaaaac cgaaacgaaa acattcacgc aaggccttcc
      961 cctttcctgt cagagaggga tacggcttat ggactgcaat gaaaatagcc cggaaaacgg
     1021 cacataaaat cggaaagaaa atccataact tgatatcttg ctgaacggac agaaaaaaat
     1081 gtgtaaacac cataaatccc ccttaaggcc gcgaagcctg ctgaatctgt tcatcaatga
     1141 gtctttgcca ttgagtccat acggcttcgg gatagtacgg ttccatatcg gacactgcct
     1201 gacgccccat agccgaccgc agggccggtt ctttcatgag tcgttctaat ccttgggcaa
     1261 aagccggaac accatcttca cataagatac cattttttcc atcaacaatc aattcattga
     1321 cggcatcagc cgaacgaaag cctacgctgg gaaggcccgt cgccatagct tctgtcaacg
     1381 ccaaggggaa tccctcaaaa gccgaaggaa aagcaaagat atcggcttgg cgcagctcat
     1441 cctggacagc cgtcgtcgtt cctgccagat gaatacgtcc agtcaggccc tgcgcctcga
     1501 tgaactggca taagcgctga taataatcct ttttctttac ttcaccccaa atccagacgt
     1561 cccactgggg atactgcttg gccaggaaag caaaggcttc gacgaggata tgcgtctgtt
     1621 tctgcaaccc atcgatacgg ccaacggtga tgatgcgata gcggtctttt acgacaccgg
     1681 gatgagcctg tttttccggg cagaccggtt cgaccacgtt aggaatccac acgaccggcg
     1741 tatgcgggag atactgtctg gtttccgcta catacgatgg catgaggacc tggatgacat
     1801 tggccttggc cttttccgct aaagcccggc cgttgcaatt ccccatgatg atacggatag
     1861 cattatgcat catagcaatg acaggaaccg ttccgtggac taaacggtcc gcgacctgga
     1921 tggcctcatg attataggca atgatacaat cgatggaatc ccccttcagc tggtcgcgca
     1981 gcttgcggac agataccgaa gcccggtagg cttcgacggg cttgtctaaa atgggaaaaa
     2041 tgcgatttac ttcccgtttt acttttacat aaaaaggaat tttcttcgta tcgacatcca
     2101 aatacagaca atgcacgcga gagtcaaaag ggaaataagg tgcggcatcg gtcttggcgt
     2161 ttgtcgccac gatgacatca taacatttct ctaccatggc attggccata ttcgtcaaga
     2221 ctcgttccgt gccggcactg gccgtcacgg tttcatcgcc catatataac agtaataa
//
LOCUS       DAWCYS010000290         2277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2321_length_2277_cov_6.009901, whole genome shotgun sequence.
ACCESSION   DAWCYS010000290 DAWCYS010000000
VERSION     DAWCYS010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_2277_cov_6.009901"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          564 a          524 c          569 g          620 t
ORIGIN      
        1 ttttattctg tcgtcccctc cctcttttct gtgttcttcc gttaggaaaa aacttcaata
       61 tgctttacat agggttggag cctgcggacg cttttctgga tatcgcgatt tgtattatat
      121 cccgggatac ggggaacccg gacccatata gaatcctgcc catagggagc atgttgtaca
      181 agatacatca gattgttcca cataaggtcc atctgcccat gagtataccg gtaataaaca
      241 tcaggattca aatctttgat gtcgatgatc cacaatgaaa tataaggcag caattcctgt
      301 acgttcttta tggatgcctg caatgatgtc tcgatacgga gagggatatc tggcagtgtt
      361 ttttgactat acaaagcaca ttctttgaca aaatactctt ggcccagcgg ctcaccaccg
      421 ccgaagacga tgcccccacc tgtcatacgc atatacagct catcccgtct gacaaaatca
      481 atcagttcac gagcactata atcaggccac tgactacgat tagtatgaca aataggattc
      541 ggacaatact gacaatttaa gggacagccg gaaaaaccaa tgagggtgga tataccaggg
      601 ccatctgttg ccatcctgaa ccgtgaaatg gagaaaatac ctgacatctg tctacctcct
      661 gtcattcatt gtcaatctgc taggcggacc ttcttccctg ggaacgaagg agacgctata
      721 tttttatgtt aatccgggtt actctaacta tgagtattat agcatgaacg attttgtata
      781 gcaaaaatgg agtatctagg acggtgccaa catattatgg gaatttcttg aataagaaaa
      841 aggtctttga aacttgacaa taatccatag tccatgctat aatgttttcc gtaccagaca
      901 catgggggta tagctcagct gggagagcgc ttgcatggca tgcaagaggt caggagttcg
      961 atcctcctta tctccaccat aaagaatctg ataaccacgc ggtttatgag ccgcgtggtt
     1021 attttttatt acatgaaaaa tcgaagaagg ttagcactag ggttagcaat aaaaaaacga
     1081 cagtaaccag caacaggaac tgtcgttttt ttattcattc tttaaggaaa agggaccgcg
     1141 catggcagta aaagtgctat gcgacggtcc ctttgcatct gactatgtta ccggagtggg
     1201 attctggtaa acgaatcagg caaccttatc tgtttgggta cggtaatagg tgttttcggc
     1261 aaagtctttg accgattcta cgtcgatcaa ttcgttttct cccaaaggtg tcttagccat
     1321 ggcttcggcc acgtaattga cgtgggtccc ggcgacccat acgccgctgg gcaggagttc
     1381 gacggcgctg cgcaggttct tcgagatgtc gtcggagaag aggtcgcggt gcatgatggc
     1441 aaagagggcg acttttccgg ctttgagccg cggcaggatg gcttgggaga cgttatctaa
     1501 tttcaggttc aggaaccaga cgccggcaaa gacgatatcg taatcgtcaa agttttccgg
     1561 ggcttgttcc atgggcagga agtccgtatc tttcggcagg accgaacgga tggcttcggc
     1621 taattttttg actctacctg tctgtgagga atacaatacc agtgctttca tagtttccac
     1681 gctcctttca catggatgag catccctttt ctctctggct ttactatatc atagaaaata
     1741 gcggaaaact gttgtattta caacacctca cagttcttca aagacgtgtt cggcaatatg
     1801 aagggcccgc agctgggatg tttttttgta ttggtgctgt tccatgcgct ggaggatgtt
     1861 ggtcaggacg gtgacagctt cgacgatgta cgggccttta ttgagcatga cacattcagc
     1921 ccgttcgccc atggcggcgt cggtgatttc cgcccgtgac ggcaggcccg tcttgaccag
     1981 gttttccagg acttgtgtgg cccagatgac ggggatatgc gccgcttcgc agacccacat
     2041 gatttcttcc tggagctcag ccaggcgccg atatccgact tcgacggcca ggtcaccgcg
     2101 ggcgatcatg atgcccaagg gctgacggga tgcagcctgg atgatgatgt ccggcaagtg
     2161 gtctacggct tctttatttt cgattttggc gaccagggcc aggtggtgga acgatttgcc
     2221 gcagcgtttc ttcaattctt tctgcaagag ggctacgtcg gctgcggact tcacgaa
//
LOCUS       DAWCYS010000291         2274 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2324_length_2274_cov_4.239297, whole genome shotgun sequence.
ACCESSION   DAWCYS010000291 DAWCYS010000000
VERSION     DAWCYS010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2324_length_2274_cov_4.239297"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          487 a          713 c          604 g          470 t
ORIGIN      
        1 ccgctcgagc tctgccatgc gcgtctggct caatccggaa aaactcgccg aacgcggcct
       61 gaccgtatcg gacgtcattg cggccatcaa ggaacagaat atgtcggctc cggccgggac
      121 cattggcagc ctgccggcac cggcgacaca ggaaaaacag tacagcgcca aagtcgaagg
      181 ccagctgtcg acgcaggaac aattcggcaa tatcatcatg aaggatacct cgaatggcca
      241 gttcgtctac ctcaaagatg tggccaatat ccagacgggg acgcagaacg aaagcagcaa
      301 ctccaaaatc aacggcaaga cagggattgg cttcggcatc cagctttcca gcgacgccaa
      361 cgccctggac acgattacgg aagtcaagaa ggtcctggaa caggccaagg aaaccttccc
      421 gccggacctg gattatacca ttgtcatgga taacacgaat ttcatcaacg catccatcaa
      481 cgaagtcaaa gatacctttg tcgaatcgct gctcctggtc atcctcgtcg tcttcgtctt
      541 cctgcagaaa tggcggacga cgctcatccc ggtcctggcc gtgccggttt ctatcgtcgg
      601 gacctttgcg tcctttaagg tcctcgattt caccatcaac accctgaccc ttttcgccat
      661 ggtcctggcc atcggcctgg tcgtcgacga cgccatcgtc gtcatcgaaa acgtcgaaga
      721 gcatatgtcc aaggataaac tgtcggccaa ggacgcgacg gaagcggcca tgaaagaagt
      781 ccaagggccg attatcgcca cgtctgctgg gttggcctcg attttgatcc ctgtcgcgtt
      841 catcggcggc gtctcgggcg tcctctacaa gcagttcgct gtgaccatca ccatttccgt
      901 cgtcatctcg gctttcgtcg ccctgacctt gacgccggcc ttatgcggtg ttctcctgaa
      961 gcccgatgat aaggccatcc aggccgggcc gctgggtgcc ttcttccgcg ggttcaaccg
     1021 ggttttcgat catttcaagg accgctacac ggggaaagtc ggctggatga tccgtcatct
     1081 gaaatatgcc gctgtcttcc tcatcctggt cacgggcctt atgggcatct gctacaaagt
     1141 tctgccgtcg acgttcgtcc ccgacgaaga ccagggcgtg tttatggcca gtatcaccat
     1201 gcccgaaggg agaatcgatt atcgccaagt tcaataagac tgtcgtcccc aaccatcccg
     1261 aagcccgcgt agcggctttc aatctggcat ccctccccgg catgggccag gaaggcggct
     1321 ggaccatgca gctcatggat aatacgggcc tgtccgatac ggaactgggc aatgcggcca
     1381 accaggtcgt cgccgcctgc tcgcagcggc cggaattatc cggtgtccgc acgagctatg
     1441 ccaccaatac accgagcgtg gcctatacgg tagaccgtga aaaaatcaag aatctgggca
     1501 tcgaattgtc cgacgtcttc acggcgctcc agaccaattt cggcggttcg tcggtcaatg
     1561 acttcaccca gttcggccgg tcgtacaagg tcatcgtcca ggccgacacg tcgtatcgct
     1621 ctcaggcaga cagtctgaaa ttcatttccg tcaagtcgtc gtctgggaaa atggttcccc
     1681 tcgatacgct gctgaccagc tccatgacca ccggcccgtc gtcaatcacc cgtttcaacg
     1741 gcgtccgcag cgtcaccatc cagggcagcg ccgccagcgg ctacagttcc ggtgaagcca
     1801 tggcagcggc cgaagaagcg gccaagagcg tcatgccgac ggggatgacc atcgaatggt
     1861 ccggccagag ccgcgaagaa agcacgtcgt ccagttcgac gacgaaaatc ctgctcatgg
     1921 ccctggtctt cgccttcctc tgcctggctg ccctctatga aagctggtcc atgccgttcg
     1981 ccgtcctgtt caccgtaccg acggggattt tcggggctgt cggctcggaa tacatcatcc
     2041 gccagctggc gaccttgttc aacgccaatg ccagcggctt cttgaacagc gtctacatgc
     2101 agattggcgt catcatgatc atcggcctgg cggccaagaa cgccatcctc atcgtcgaat
     2161 tcgccaaaga gcgtgtcgac aagggtatcg acccgatgga agctgtcgtc atcgcctcca
     2221 agcttcgcct gcggccaatc ctcatgacgg cctttacggc catcatcggc tgcc
//
LOCUS       DAWCYS010000292         2272 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2329_length_2272_cov_4.534957, whole genome shotgun sequence.
ACCESSION   DAWCYS010000292 DAWCYS010000000
VERSION     DAWCYS010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2329_length_2272_cov_4.534957"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          533 a          503 c          524 g          712 t
ORIGIN      
        1 tgacctgggt catctaggct ttgtcaacgg ttttgctgct gcgaatgacg atgacctgac
       61 ctggactttc cttgacagca tctttcatgg tttcgccaag tttaacgggg tcattaacac
      121 tacccaaaat gcctgcagcc tgcgtatcat cactgggacg ataggaattt ggtcgccgat
      181 tgtgattgtt ccggctccag cttgtgcatt caagcccaaa gtaatggaat cagccccacc
      241 tgctccttag ccaccatagt tagcatattt gctatcgtcc aacagtccat ccgtgcgttc
      301 tacaccaaca aagttagaac catcagtctt ggttttatcg gccgcttctg cggtcatcgg
      361 cataaatacc gatgcaactg agaacgtccc cagtgccagc agcacagctg ccgtcaatgc
      421 tgttttattt cttgccgtat gactacctgc cgttttgcag tgacttttag ccagttctga
      481 aacgacgacg taacagtgtc tggttttgct ataaataact ttaaatatct tattcatgtg
      541 ttcgcacatc ctttgctgat aatgatttca ccgtatatat catgtaagat tttttcgatg
      601 caccttcttt cattattttg catttttatg taaataatat gtgtattttg tgacatcatt
      661 atagcattta tttttctttt taaaaggctt tattacgcga tttatctgct atttgcagta
      721 gtcattgctc ttgtttgtaa ttatattttt attctttatt ttgtaattag ttaatatata
      781 gtattaacat tgtagaaaca aaatatgaca caaaaatttt tgtaaaaaaa agagcattcc
      841 atgaaatccc ttatcaaggg tttcataaaa tgctcttttt ttgaataact gcgacttttt
      901 tacggctgcg ggatatgttg ggccaaatct tcggtcatct ctatgtgttc aaagtattta
      961 tgggtcttcc acaggagcgc gctgaaggcg ctgaaggtca gacggaaccg gaagtgtttg
     1021 aagctgatag ggccttcaaa catggggacg cggtccaggt catacgggtc gtcccagggg
     1081 aagtcgcggc ctgtttctac ggtgaaagcg tagcggtagc cggctccccg cgtttcttcc
     1141 atgaccatat cgttatattt gccttccggg aaggcgatga agtggcacgg tttccctgtc
     1201 atcttttcga tgatgtcttt ggaatcctgc agttcgtgac gcacgccagc ccggtcaaag
     1261 ctggtcaggg gcttatggct gatggtgtgg gacccgaatt ccatgccgtc ggcggacatt
     1321 tccttgacct ggtcccaggt caggtagccc ggcgtattca tgaggttgat gatcaggaat
     1381 atggtgccgc gcatgtggta tttcttcagg atgggatagg cgttttcata gttgtcgacg
     1441 tagccgtcat caaaggtgat gaggactggc cggtcgggca ggtcgccttc gccgcgcatg
     1501 taggcatcga actgttccag cgtaatggaa tggtaatcgt tgtcgtgcag atatttcatc
     1561 tgttcgtcaa agttagccgg cttcatggtc agcacggtgt tgaatttatt attgacctga
     1621 tggtagttca ggaccgggac gcctgtcagg tgatagttcg tcgacccgaa ataccaaaaa
     1681 gcaaagatac cgataaggag gaccaggacg gtgagaatga cgccgatgat ccacgagcat
     1741 attttcttca acataagact tcctttattt tgttacgttt ttcagggtct tgatgaaatg
     1801 gttctcagtc agccattttt catcgccttt ttccagggct tctttcagct ctttgataca
     1861 gatattttcc atatggatcg tttcgatggt cgtcgtcatg tatttcgttt cttcatacat
     1921 gtaatcatcc agttcctgct tgaccaggac gtctttcagt tcaaagagca ggtcttccga
     1981 ttcctgctgg atggccagca gttcggtcca gtggttgtat tcttcttcgg tcaggacgaa
     2041 ctcgccgtac atgtccgtga ccagctgttc ctgggaacag aggatctgtt tggcataatc
     2101 gacgcccgta gaccggtcat agacatggag ctcacggttt tctccattga cactaacgcg
     2161 catggcgtaa cacctccgct taagtttaat caaagtacat acatcctcta ttatacagga
     2221 ttttgagaac acgacaagtg ttctttggca taaaaaaaaa gctttatgat ca
//
LOCUS       DAWCYS010000293         2267 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2332_length_2267_cov_4.641501, whole genome shotgun sequence.
ACCESSION   DAWCYS010000293 DAWCYS010000000
VERSION     DAWCYS010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_2267_cov_4.641501"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          659 a          550 c          500 g          558 t
ORIGIN      
        1 tatcatgcac tcttaaaatt ttcagtactg gtctgaattc acaggcccat tatagggtaa
       61 ataataaatc gggatttttt attttaaatc ccgacctctc ccaccaccgt gcgtaccgtt
      121 cagtacacaa cgtttctcta gtttttacaa atttgcgcat aatagtccag caaggtgatg
      181 tatccttgcc gggctatttt tttatttatc agaatggagt tcagcatgag tgcggtttat
      241 tatcgaatta tgttataaaa ccaacatact tgtctcccct gccatgctat actcattcca
      301 gaaagacgaa gaaaacaata aaaaatgcct gtaatgaccg tatcatgaac aggcaacaga
      361 aaggggcaga cctcatgaaa aatatagaat ggaaagataa agtggcaagt tttaaaaaaa
      421 ttgatgtgcg cggcatcgcc ggtaatttcc ttgatgggct gaaaaaacag gcagccaagc
      481 ttcccgtcgg cgaagggatg gaagtcattc aaagttttga accaatcccc ctctatgaga
      541 tcatggaaat gctgggatat gagcattata cagaaaaaac agcagaagat gaatatcacg
      601 cttatttcta tcggaccgag gaaaaaggga acgtggaaga tgcgccagaa cggccggcgg
      661 tcatcacgaa ctatcccatg atcgatgaaa aactgggcga actggctgta gaattttggg
      721 atatgacctg gaaaagcgaa aaacgatatc tcgattataa catccgcctc ctgctttcgc
      781 tggccaatgc cgtcggtgcc ggacggatgc ggcaggccat gcgggaactg ctgaaagcct
      841 atgccaatgg cctggactcc agagccctgg acgacgtatt cgaacagctg gcctggaaca
      901 tgggcatcgg cttcttcagt tctgaaatcg ctccgtcccc actgtttcat gcgtataaat
      961 tgatcaaaca aatggaaaaa cagggaaaag aaagaaatga aatcaaccag atgctaagag
     1021 aaaaattatc cgataaaaaa ggaggatgca agtaaaaaaa cagaggctgt cgcattaaga
     1081 cagcctctgt ttgagtttta agtttgtagt ttgtagcgaa ggtttttaaa tttaaggccc
     1141 ttctcaacga tagaattcag gccgacgcaa ggtaatatag ggccttgcct ggcggcttgc
     1201 tttgcatttt tccaagtcga tatcgcaata taccagtccg gcttccccac ccgctttgaa
     1261 cagtgtctgt ccttcactgt cgacgaccag ggactgaccg gcgaaggtaa cgtctccttc
     1321 ccggccgacg cggttgcaca tggcgatgaa gacattgttt tgataggcct ggacccggat
     1381 ttcccattca aagaggtcta acggttctgt cgtcaaattg gccgtcggta tcaagaccaa
     1441 ttcggccccc atggcagcgc aagtgcggat gctttccggc aaatggcggt caaaacagat
     1501 gacgacgccg attttcccaa aagaagtatc ataggcgcga aaaccatctg gcgaaggcgt
     1561 atagtaatcg agttcgtaga actggggcgc actggtgaca tggaccatag ccgattttcc
     1621 cagcagattc cccttttcgt cgatgaacaa agacgtatca taggcctgcc cccgatattg
     1681 caaatagaga ttaggcgaca ccatgcaatg tgcctgccgt gacaggtcat aaaaggccgt
     1741 cacccgcggg tcatcagggt ccagcagata atccggcagc tcgtctgccg tgtactgcgg
     1801 caaaaaaggc atatgctgca cttccggaaa gaagagcaga tccgccccgg ccgcttcctt
     1861 cacagcctca taactcacct gtaaattccg ttccatatcc tcagtcatcg ccatctgcat
     1921 catcgcaatc cgcatcaacc tcgctccttt cactctgcct gttttacaca aattttctta
     1981 ccacaaaagg aaatgccata tttccagact ttctggattt cccgattcgg aatcaccaat
     2041 tcacaccaat aaccgccaaa ggctttttcg caagatatag gagtcaaata tcccgttgta
     2101 agcagcatcg tatacagcgc atcttcatca ttaccgattt catcataaat aatgccttcc
     2161 cgaatcatgg ccgatatgct ttctccacgt aaaagagaaa tcaaatcatg ttccttttcg
     2221 tcagacagac acagcatcag ctgattcaaa attgaattcc ccgatgt
//
LOCUS       DAWCYS010000294         2259 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2345_length_2259_cov_5.208258, whole genome shotgun sequence.
ACCESSION   DAWCYS010000294 DAWCYS010000000
VERSION     DAWCYS010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2345_length_2259_cov_5.208258"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          436 a          611 c          725 g          487 t
ORIGIN      
        1 cctgcgggtc ttcaaggttc agcagctgcg cgaaatactg cagcaccgag cgctgcatgt
       61 agggatggag gtgcatttcc ggttcatcga tggagatgat gaggggcagg tagcgcttcc
      121 cttcttcgtc gacgatgacg ttgtgttcca acggtacagc gcggctgatg gacatcatgt
      181 agacctgggt gatgacgcac agggcggcca gggaaatgaa tttcatgttg tccgccctgg
      241 gccggtccat gcggcagagc atgcgggaca agaggaagat gttgaggaaa tacgatgaat
      301 cgaggctgcc gacggcgact tcgtgatgga tgaaggcctt ggcttcttcc gacaggccgt
      361 cccagtgagc ttcgctccat tgggagagct tctgttccag ggcgcggtaa atacgcggcg
      421 gcacgcgctg ttcttccggc atggccgccg aataggacac gtagcgcagg cggcggatga
      481 gttctaacgg cagttcttcg ccgctgttgt cgtcgtagag atggggatac atgtcgctga
      541 catgcatttc catgcggacg cgcaggctct gacggtgctc gcccggcgta tcggcgaaat
      601 actccttctc gctctggagc aggtgcaggt ctaaggtgat gatgatgggc cgggtcacgt
      661 cggcgtagtc gtcttcttcg acattgcggc cggcactcat gtaggaaatg aggcgcagca
      721 ggccgctctt gccaatggcg ttttcaccga cgatgtagtt ggccctttcg tggaaagtca
      781 tttcgacgtc ttgtagattc cggtaatttt gaatacgcat gtaagtaata tacacaaaat
      841 cacatccttt tcaacattac ttttactata cgtattcgac aaaaaacgcg ctactccctt
      901 cttttagaga gcagcgcgtt attcctttta cgtatcgttc atggtttgat gaacggccta
      961 tgggttcagc aaacatcgtg aaaaacgatg tgaccaatct ttggcgggcg ccccacgtgg
     1021 tcgcccctac gatgaatggc ctacgacgaa cgacctacga tcaacggccg ctttcgcggc
     1081 acactttggc cagccaatgg gcgatgtcga aggtcgtctg gccttctttg agctgccagt
     1141 gccagttgcc gtcggcgacg ccgggcgtgt tcatgcgtcc cgacgacggc agggccagga
     1201 ggtcctgcat ggggatgatg actgtttcgg cccggcggca gtaggtatag gcgatgaggg
     1261 cgccgacgat ttcctgcggt gccgcttcat cggacaatcc tagcatctgg cggacgtgga
     1321 gctgttggct cggcgtcagg tcgtcttcat accagccgcg gatagtgttg ttgtcatggg
     1381 tcccggtata ggcgatgcag tccggctcgg tatcgaaggc caggcggccg tcggcccggt
     1441 ccttgaggtg gaactggatg actttcatgc ccggcaggtg cagggcatct ttcaaggtgc
     1501 acacatcgtc ggtgatgacg cccaggtctt cggcgaccag gcggatcctg ccgattttct
     1561 gatagaccgc ccggaagagg tccaggccgg ggccatcttt ccaagcgccg ctcttggccg
     1621 tttccgaacc ggccggtacg gcccagtaag cggcaaagcc gcggaaatgg tcgacccgga
     1681 cttcgtcgac gaggtccatc atgcggcgga agcggtcagt ccaccactgg tagttgtccc
     1741 tggccatggc ctggtaatca tagaggggat tgccccagag ctggccgtcg gcactgaagt
     1801 agtcaggcgg gacaccggcg acggacgacg gattccctgc ttcatcgagg tcgaactggt
     1861 cctgatgggc ccagcagtcg gcactgttat gggcgacgaa catgggcacg tcgccgagga
     1921 tggacacgcc gttttcagcg gcataggccc ggacatcgtg ccactggcgg ctgaagatgt
     1981 actgcatgaa tttgacatgc gatacggcgc cggccagctc cttgccatac tgggccaggg
     2041 cttcccggct gtgatggcgg atgtcttcgg gccattccgt ccagcaacgg ccgccgaaga
     2101 agtcgcggac agcccggaag agggcgtagt catcgagcca gtaggcattc ttttcacaga
     2161 aggcccggaa cggttcccag ggaatggtca ggtccggatc aagtgacgga tcccagagga
     2221 gggaggcctt ataggctgcc cggctgtgcc gggccgccg
//
LOCUS       DAWCYS010000295         2256 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2349_length_2256_cov_6.072240, whole genome shotgun sequence.
ACCESSION   DAWCYS010000295 DAWCYS010000000
VERSION     DAWCYS010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2349_length_2256_cov_6.072240"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          501 a          568 c          642 g          545 t
ORIGIN      
        1 atgcagtaga cgtggtggcg cgtcgtgcca tagcggtcgg cttttttgcc gccggcgttg
       61 gtagccaagt tgccgccgat gaggcagctg tcggagctgc acgggtcacc ggcgtagagc
      121 agaccctttt tgcgggctgc attctggatg tcgatagtgc ggacaccggc ttcgatggtc
      181 atatacatgc catcttcgtt caattcgatg atgtggttca tccgttccat gagcaggacg
      241 atgcctttat aagccggaac ggcgccgcag ctgacgctgg tgccggcact gcgcggcgtg
      301 accgggacgt cgaagcggtt ggcgatcttc atgatttccg aaacttcctg ggtattagcc
      361 ggcaggacga cgacttccgg cgtcttccag aaggacggtg ccaggctttc atcggattta
      421 tagatgtcca ggacatcggc atcggtcttt acatatttat caccgacgac ggctttcaaa
      481 gcgtcgatga cttcagctgt aaccggatta tattctttca ttgtaagaac ccctttactt
      541 tttaatgaaa aatggaacca tcatttcact tattgcaact atttctcgtg aattatgaat
      601 attatagtat acaaggtgcc atttgtcttg gaaattgtaa aaaaaagata tacagtttag
      661 tcattttaat acatgcacaa acgtaataaa tatggtaaaa cgtataaccg tagagcataa
      721 aaggcactgt cacatgaaga cagtgccttt ttccgtaggg gtcgcctaaa aggccccgct
      781 gtgaatcgtg ggtatgagca tttgggtata tgctacgaac cacgaacacg atcgtcccac
      841 aagcgtgccg aacaggacaa gcccctactg ccgaacgggg aaagtggcgg cggcgtggcc
      901 catgacagtg actttgtagt cttccggcag atcggcctgc gctttttccc aggcttcttc
      961 cagggttgct gccgggatga ggccgctgtg acggatgatg tcgaagtttt ccggccgcgt
     1021 gacgacgtat accgccttca tggagcggaa aatgcagtgc gacttgaagg cgacgaaggc
     1081 gggaatggaa aagtctgccc gcaagtccct ttcaaaggct tccatgtcgc tgcggaagaa
     1141 ccagtccgta aagatggccg gttctttgat atccgggcag tccagggtca ggatcatcgt
     1201 gccgcccggc ttggtggcga agacggcgtt catgggcgcc ttcatgctct ggtagaggtt
     1261 catgtccttg ggatagccgc cggccgatgc gatggtaata tccgccagtt cctggatggg
     1321 aacgctggcg atgtgcagca agtcgtcaca gccttttttc caggcatcgt acaaagggcc
     1381 gccgacgact tcatagagat cgccgtctgc agaaaagacg gtgttgacca gaaaacaggg
     1441 attgagcagg gctgcggcct gtttcatatc gtcgtggatg ggattgtctt ccaacagact
     1501 cgtttcgcat cggggattgc agccgccgcc aaaggtatcg gtcaaggcca tggcgtggtt
     1561 gcgcatgatc gtttcatagc tggcaacgcc gggcaggacg gctttgcggc cgccgccaaa
     1621 accggccatg ggatggaagg atacggcgcc ggtcaagatg actttatcgg cctggacgac
     1681 gtgcgagtcg agatagacgt cattgccatg cgtcgtcgtc ccgattttgg tcagctgcga
     1741 cgcatcgcgg cagtcatggc tgacgacttt gacgcgcttg accatgtctt cgccgcagac
     1801 gatgacgtct tcttccggcc tatgggcccg gtgcgtcccc tgggccgtga cgacggtaat
     1861 ctgttcatcc cggacgccgg cctggttcaa ttcatcgacg atgaccggca gcatctgtgc
     1921 cgtatggacc agacgggtaa tgtcactgac gatgatagct accgtatcgc cggcctggac
     1981 gacgtctttg agcggtgcgc tgccgatagg gtggcgcaga gcctgtcgga tggcttcctg
     2041 ttcatcggct acggcatcga cgggattgcc gttcaggtca tacaatacgt gtgcttcatc
     2101 gagcgaaacg gtctgtgtac ccttcccaaa gggaagagaa aaagtcttca tcataaaaaa
     2161 tacccctctt taatataaat tttaatataa attctttaat atcagcactt tacaactttt
     2221 acgccgcctc ggaagttgac gaagcgagtt tacaca
//
LOCUS       DAWCYS010000296         2250 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2356_length_2250_cov_3.771754, whole genome shotgun sequence.
ACCESSION   DAWCYS010000296 DAWCYS010000000
VERSION     DAWCYS010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_2250_cov_3.771754"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1884..1893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          401 a          650 c          619 g          570 t
ORIGIN      
        1 ccttgcgacg cttctggtac atcctgtcag aatctgcaat agacttctgg atataccgtt
       61 cctgtttctt ggtcaactgg tcgagctgct tttcttcctg cgccgtcgag ccggccttct
      121 ggcctgcgcc tagaacgttg cgggccgtat cgtacatatt cttgccggcg gttacggctt
      181 tactggccat ctgcaaggat ttgtaaatgg ccagtagttt cgtaccctcg acgatgaccg
      241 tgctgatttc gtttttattc tgggcgacaa tcgtggctac tttagcgagc cccgcctgca
      301 cgctcggcag gatctgcgat accaaaggag ccagcgcaga cccggcgacg acaccgagct
      361 tgctgaactg catgctgact tcctgcatgt ccatataagc tttgtgcatc tcttccgggt
      421 tcagtccgac gcctttgatt ttagatgccc gttccgctgc ttccgtataa ttgtccaggg
      481 tcttcgtaag ggccaggcca cggacgccga gggtgttcat caggaattcc tgcccctggc
      541 ctgcggcctt cgccttttcg tagcccttgg cgagttcgcc cagctgttca ttcagcggtt
      601 tcaagcggcc cgacgagtcg gtcatcgaga tgccgagctg ctgcatgatg ctgcgagcct
      661 tgtcaccggc tgggcccgac gacgaaaggg tcttgtcgaa gcgcatcata gccgcggccg
      721 cggtatcgac gtcgccgccg gtcagcttca tgaccgcgtt cagctggcct gcctgggcgg
      781 cggacatgtt gtatcgctgt cccagctgat aaacggcttc tccggcatta acggcacttt
      841 ggacgacggc gttcaagccg aagccaccgg cggcaatgct ggccaacttc gtgaggttac
      901 cagtcaggcc gctgattttc ccggcaactt cgtctacgct gcccgaaaat tctttcaccg
      961 ggctcacgct gaaggtctgg ttcagcgcct ctttcgattt attcagctcc tgggacagac
     1021 ccgtgctgtc cgccccaatt ttgatcataa ggtctgctaa ggtggccaac gtctacccct
     1081 cctctttcgg catcatcgat ggaagcccga cggctttcag gaattcttcg cgctccaggc
     1141 gcggatccgg cttgtcctcc ggatgcagtc catagtagat agagtcaggc tggacaacct
     1201 ggctggtcct acagctcatg atgcaggccg tgaaatagga ctgcttacgt tcctgctctt
     1261 tcttgcggag cagatagccc tctaccattt cattaaactc atggatctgc aaggcctcga
     1321 actcatccgg tttaagtgcc agcggcccat aggccaccgg ctcagcgttc tttatccatt
     1381 cttgtgcgga gccgacgacg acactgcctt tttcttcgtc tccgtcttgg cgtttttttc
     1441 cggtaccctc acacgggtgt agagtccggt ctgcaagatg gctccgatga agttggccgt
     1501 catgtcgtcg atgctgttcc cgtcatcgca ataggcctgc aaaaggtcat agacctggtc
     1561 gtcgttgcgt atgccgtgca gtttatcgtg gatgccccag cggagcatgg agaccagtac
     1621 gtcgaggttg cagctggcta ccagctgacc gacaccgtca cccatgatgg acaggatgga
     1681 ccggccgact tcgcgttcga tgtggcgcag gtcgccgata gtcagatata agctgtaccg
     1741 ggtcccgctg atggtaatat caatcgtctc tttcaaaatg catcgctcct tgtgcgtaat
     1801 aagggcgtcg cgtgacacga cgccctttcc tgcttcctac gcagaaatag tcacattcac
     1861 ggcgacatcg aggtcggacg tcannnnnnn nnntattcta tcttgatggt aaggacgcct
     1921 tcgacatacg agtattctac ttcctgcgtc agcggaacgt tgtcgcaagt gatgctgcgg
     1981 acaaaagcgt cgaccggcgt gacgttgacc gtagcgtctt tggcgctggc cttggtgaag
     2041 gtgatcgtcg gcgatgcgat ggccggcgtg ccgaccgatt tgataccgct gatagcgccc
     2101 ttgccctcta aggtcatctt gagcgtcgat accccgttgt agctgtgttc ttcatcgagg
     2161 ctcgtaatgg cggcccagcc ggtacgatac gatccgtcgg ggtattcctg gccgatgtaa
     2221 atcggctaga gcgcttcgta gcggttgttg
//
LOCUS       DAWCYS010000297         2248 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2358_length_2248_cov_5.314637, whole genome shotgun sequence.
ACCESSION   DAWCYS010000297 DAWCYS010000000
VERSION     DAWCYS010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2358_length_2248_cov_5.314637"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          480 a          669 c          618 g          481 t
ORIGIN      
        1 tgttcccggt catcacggcg gccctggtct tgtcgtggat cgtatcggtc atggtggcgc
       61 cgctcttcgg ctattacctg atcaagccga aagtggccaa aggcgaagag ccggagccct
      121 tgtatcagag ccggttctac gtcttcttcc gcaaggtcct gacctggtgc ctgacccacc
      181 gggtattagt catcggcggg acagcggcta tgttcatctt gtccgtcggc atgatgaaac
      241 tcattcccca ggaatttttc ccgccctccc tgcggccgga actcatcgtc gaaatgaccc
      301 tgcctgaagg ctcgtccatg caggccacgc agaaggaagc atcccgcttt gcccagtatc
      361 tggacagtca gcaggcgaag ctcaaagatt attcgtacta cgtcggcgaa ggggcaccgc
      421 gtttcgtcct gaccatcgaa ccggtcctgc cggccaataa ctacgcccag ttcgtcgtcg
      481 tcgccaagga tacgcagacg agggaagaat tgacgcagtc catcaaacag gaaatggccg
      541 acaagttccc caacgtccgc tgcaacgtca agaacgtgcc cttagggccg ccgtcggatt
      601 accccatcat gctgcgcgta tccggctacg atacggataa ggtcaaagcc tatgcccagc
      661 agatcgcggc gcgcattgcc aaggacccga atacggaaaa tgtcttcctc aactgggacc
      721 agaaaaataa gatcatgcac gtcgacctgg atcagaataa gctgcgggcc atgggcattt
      781 ccggtgaaga cattgcccag accttgtata cggaactgtc cggggcgacg gtcgcccaat
      841 actatgcggc cgataagacc atcggcatcc agctgcgcct ccaggacagc gaccgcaatg
      901 acctggccaa gatcaagagc ctgcccgtct acgtaggcca ggccggttac gtgccgctgg
      961 aacagattgc cactatttcc tataaaggcg aagatggcct catctggcgc cgcgatttga
     1021 aaccgaccat caccatcaac ggcggcatca agtccggtac ggccaacgat gtgacgcaga
     1081 aagcctacga ttccattgcc gatatccggg aaagcatgcc ctttggctat accgtcgaag
     1141 tcgacggggc cctggaaaac agccagaagt ccctgaagta cctcctggta ccggtaccgg
     1201 tcatggtcat catcatcatg accctgctca tgttccagct gcgcagtccg tcgctcatgt
     1261 tcctggccct gattacggcg cctatgggac tcatcggcgt ctcctggggg atgctcctct
     1321 tcgggaaatc cctgggcttc gtcgccgatt tgggcatcct ggccctgggc ggcatgatca
     1381 tccgcaactc cattattctc ctggaccaga tacagaaaca tatggctgcc ggcgaagacc
     1441 catggcatgc cgtcatcgac tcggcggtca tgcgcttccg gcccatcatg ctcacggccg
     1501 cagcggccat cctgggcatg atacccctca tgaagagcgt cttctggggc cccctggccg
     1561 tcgccatcgc cagcggcctc ttcgtcgcca ccgtcctgac cctgctggtc ctgccgacca
     1621 tgtacgccgc ctggttcaaa gtaaagagaa cgagttagct tagtggctcg tggttcgtgg
     1681 ctcgtagggg ccgtcctgaa aggcggtccg caagggctct ccttatagga gagatgtcag
     1741 cgaagctgac tgagaggttg ctcttcatag tttgtagtac ggagtttgta gaagatagtt
     1801 ttaaatttaa gaccatccgc taaccactag ccactagcta ctaatcactc aaaaaagtcc
     1861 tgtcgcgcat gcggcaggac tttttttgcc atgctcccaa cctacatgtg ctcttacagc
     1921 agcccctttg aagtcaccct gccggctttg gctgccgccg ctcccggtgc ccgcctcttt
     1981 acggggctga ccctggataa ggatacggat tacagcgtca tcgatccctg gctggataaa
     2041 gccggattgt tataagacag gagggaatac agatgtctca tgatatgagc cggcggactt
     2101 ttttgaagct cctcggcggg ggactggctg gagcggctgc cggcggggcc ttcatgaagc
     2161 gcgaggatat cctggctttt ctcgatgccg ataaggctgc cggggaagcc gggcaggatt
     2221 tgggaaaagt tacgacgcgc tattataa
//
LOCUS       DAWCYS010000298         2245 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2359_length_2245_cov_5.941096, whole genome shotgun sequence.
ACCESSION   DAWCYS010000298 DAWCYS010000000
VERSION     DAWCYS010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_2245_cov_5.941096"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          409 a          635 c          703 g          498 t
ORIGIN      
        1 ccagcacttc tttgccgtgg gctgtgtttc cgcgcaggac gaggccggct tcatcctttt
       61 cgcacagcgg ctgctggagc ttggcggcga cggcgcagaa actgggcacg ccgggcacga
      121 tttccgtgtc gtagccggcc tcatggacga ggcggtggac gtagatgctg gtcgcgtaga
      181 ccgtcgggca gcccaaggtg atgaggcaga cgtcgcggcc ctggtcgagt tcgtcctgca
      241 ggcggcgcgc cgctttctca tgggctccct ggaggacctg gtcgtcgcgg gtcatgggga
      301 aatccatggc gatgaccatt ttatcttcta aggaaacgcc gcagccgtcc agggccttca
      361 agaggatgcc ctgggccgtg acgacgccgg tccggctctg gggcacggcc aggacgggac
      421 aggtcctgat gattttcagc gccttcaggg tcagcagttc cgggtcgccc gggccgacgc
      481 cgacgcaata gagtgtacct ttcatgatca tccctcaaat aacgaatatt tctgaccgtt
      541 ttccttgcgg acgaaaatac ccgtccgccg ggccagcatc atgtccgtgt ggatgggcag
      601 gccgaattgg ctgcccagga tttcccagac ctgggacggc ttgatggccc cttcgccggt
      661 ctgatagatg ccgacagtca gatagacatt gccgtccttc acatggccgt ggacggcttc
      721 gacgacgtgc tgcttgacgt cgatggtacg cacgcggtgc ttgcccttgt gggacacctt
      781 ttcatagagg acgctggctg cgtcattgaa tttcgccaaa atctggttga gtccgtcctg
      841 gctgaggtct tccgtcagcg ggccgcgcag ggtatagacg gcgtaattgg caatggccat
      901 cagcttcagt gccttggcat cgacgtattt gccatcgagg acggcaaagc cctggggcga
      961 catggcgttc atgcgcgcca tgacgtcggc gacgggcagc ttttcagcca gttccatgtc
     1021 gaggtattcc acatcggccg agacaccgac gcccagggcc gaggcgaagc tgagcttcat
     1081 atgcggattg aagccttcag aataacagat aggcagtttg gcccggatga tgacgtagcg
     1141 cacggcccgg gcaaaatcca ggtgcgacag gaattgcagg gccccatctt tcttaacagc
     1201 taaacgtagt ctttccatgg tcgtcccctt cctttctgtc gatgatggat acgcccagat
     1261 tcgggcagac accgcagccc gtacagggca ggcggcggca gtcgtgagtc agttcagctc
     1321 gagaggcctt ctgccattcg cgcttcaaat aggccttggc ggcgccgttg ctgagatggt
     1381 cccagggcag ggcttcgtcg aggctgcgcg tccgggcggc atagtaatcg gggtcgatgc
     1441 cggccaggtc gaaggctttc atccacatat cgtaatcgaa catttccgtc cagccgtcga
     1501 acttgcagcc cagtttccag gcttcgtaga ggactttgcc catgcggcgg tcgccgcggg
     1561 cgaaggtcgc ttccaggcgg cccgtcttgg catcgtggta atggtaggaa atgtgtttgt
     1621 cgttgatgag acccttgatg tactgctgtt tccgttcgat ttcttcaatc ggcagctggc
     1681 cgaaccactg gaaggccgtg tgcgacttgg gtacgaagct cgatacggag accgtgacct
     1741 tgccgttgtt cttgtgggtc actttgcggt agagattgag gaccttgtag gccaggtcgg
     1801 cgataccggc cagatcttcg tccgtttctg tcggcaagcc catcatgaaa tagagcttga
     1861 ccgtggacca gccgttttca aaggcatttt cacaggctgc cagcaaatct tcttccgtaa
     1921 cgcccttgtt gatgacatcg cgcaggcgct gggtcccggc ttcgggcgca aaggtcaggc
     1981 cgctcttgcg gacctgctgg accttcttgg cgatatcgac ggaaaagctg tcgacccgca
     2041 gcgacggcag gctgacgctg actttctggc ccttgaattc gtccaagagc tggtcgacca
     2101 gttcgggcag gcaggaataa tcggccgagc tcaggctcat gagggaaatc tcattgtagc
     2161 ccgaattggc gataatcagt ttggccaggt cgggcacgtc ttccgtcgat ttttccctga
     2221 ccggccggta gagcatggcg gccag
//
LOCUS       DAWCYS010000299         2237 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2371_length_2237_cov_5.128781, whole genome shotgun sequence.
ACCESSION   DAWCYS010000299 DAWCYS010000000
VERSION     DAWCYS010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_2237_cov_5.128781"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          625 a          612 c          489 g          511 t
ORIGIN      
        1 atatcatgca ctcttaaaat tttcagtact ggtctgaatt cacaggccca ttatattaag
       61 tttgcagttt gtagcaaata ttttcaaatt taaagccctc ttctacaaac tacaaactcc
      121 gtactacaaa ctccgtacta caaactttat ttccaaaatt ctttggcttt agcgatgttg
      181 gcgcgaatcg tagcgcagca ggctttgaag tccttcattt cctgttccgg caggttatat
      241 tcgatgactt cttcgatgcc attggcgccg atgaccgacg ggacaccgca gaagatgccc
      301 gtttcgccgt attcaccatc taaaggcgcg ctggctgcca tgattttccg ttcatcccgc
      361 aggacggccc ggaccatggt agctgccgtc atactgatgc cgtattccgt gcagtgcttg
      421 ccagaatagg tgacccagcc ggcatggatg gtttcatcct ggacgcgggc cttatcgaag
      481 acataggccg gattctgttc cagttctgcc agggatttac cgccgaactt gatgagcgac
      541 caggggacga tctgggaatt gccgtgttcc cccatcatgt aggctgagaa ggaatgatga
      601 tccaagccgg tctgctggga aatggcactg accaggcgcg cgctgtccag ccccgtgccg
      661 gtaccgaaaa catgaccttt cggaaggccg ctcttcttag cgatgaggtc cgtaatgacg
      721 tcacaagggt tggtgatatt gatgatgatg ccctggaagc cgccggccat gaccttgggg
      781 atataatcag cgacctgagg cacagtgaaa tgcatttcat cgtcgcggtt ccccgatgcg
      841 cagagcgtaa tgtcgccgat agcattgacg atgacgtcgc agtctgccaa ttcttcatag
      901 ctggcaatcg tgtaattgac acgatgaggc atgaacatga cggcatccat catatcctgc
      961 cgttcactga ccagtttttc cgacttcacg tcgcataact tgacttcatc ggcaatcccg
     1021 aggaccccta aattaaaagc tacatgagcc ccgacatgac ctaagccgat gacccctacc
     1081 acacgtttct taaaagccat acaaaacgcc tcctgttcac tatagcatat agaaaaagtc
     1141 tatttatata ttatatcaaa aaaacaccct ctttgtccgt tgtaattaca acaatcccta
     1201 ggctctcaat tttacctatt tatttcatac tttatacaaa tccaaaaaaa tccgggccta
     1261 taatgcagta tgtaaaaatg tcaactacgg tacaggaggc caggaatcat gacaagccca
     1321 agccgtccaa agcccccgct gggacgctac ggtccgccag ggaatcaacg acttcatcgc
     1381 ggcttacggc aaagattcgc cgaactacga taaaaacaat catccctatg ccgtcttcga
     1441 tttcgacaat acgacatcca tcctcgacgt aggcgaacag ttggaagtct ggcagctcga
     1501 tcatctgcaa ttcgccgtcc cgccgacaca gatgaagtcc gtcctcatga cggggatccc
     1561 agctgataaa ttagacgcca tccgcgcagc cgtttcctat ttcaaagtcc ctggcgtcaa
     1621 agatgtcctg gccatgacca acaaagtaga cgccaatggc cgttacctca atcagtacga
     1681 ttacgacctc catccccaga cccagggcgt cggtaaagcc gaaacactgg caaaaatcct
     1741 ggtaccgaaa tacaaaggcc acggccccag cttcacggct atggattccc agggggattt
     1801 caacttctgc accgaattca aagacacgaa agaagtcttg gtcatcaacc ggacgcgcac
     1861 cgatgacgct gccctctgcg ccggcattgc agcttaccag aaaaaacacg gcatcgacct
     1921 cgccgcagcc aacaaaaatg gcgacagcct cttcctgatc cagggccgta acgaaaacaa
     1981 aggcgaattc tggaacgacg acaagaccct tctcttaggc aaaaagacgc atcctacctg
     2041 tccgataaag ccaccaaagc cgaaacagaa ctcgaacaag gaaaaaccat cgtccaagta
     2101 ttgaaagaca atacgaaatt aaaagacaaa tatcagggat ataagacgcg ggaaactctg
     2161 attatctcct caaaaaaagg actagcacat gatatggtcg aaatcttcat gcgctagtcc
     2221 ttttaacatt gatagag
//
LOCUS       DAWCYS010000300         2222 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2382_length_2222_cov_6.260729, whole genome shotgun sequence.
ACCESSION   DAWCYS010000300 DAWCYS010000000
VERSION     DAWCYS010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2382_length_2222_cov_6.260729"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          490 a          574 c          574 g          584 t
ORIGIN      
        1 ccatggggtt cacgacgcgc tttagcaggt tccgcgtatc gtcgaacctg gccgccgttg
       61 cgcctgcgaa gccgacgagg atgtccgccg tcaggaccag gtcgggcatg cgcttgcggg
      121 catagtcgac gagttccagg taatgttcca ccgtgtagcc gcggttcatc ttcttcaaga
      181 gctcgtcaga accgcactgg atgggcagat gcatgtggtt gacgaccttt ttgctgtcgg
      241 caatggcgtc gatcatggca aaagtcatat ccttggggtg gctggtcatg taccggaccc
      301 gttcgatgcc gtcgatatcg tcgacagcgg cgacgagctt ggagaaatcc gtgccgtcct
      361 tgaggtccag gccataggaa ttgacattct ggccgagaag ggtgatttcc ttgtagcctt
      421 cagcagcgac cttcttgatt tcttcgccaa tggcgtcgat gggacgcctc acttcacggc
      481 cccggacata aggcacgata cagtacgtgc agaacttgtt gcagccgttc atgatgggga
      541 tccaggcaaa gaatttctgg aagcgcttga ccggcatatc atggaaagcc ggcagggtca
      601 tgtcggcagc catataatgg tcggccttgc tgcgccgttc ccggatgagc tcgatgagct
      661 tgtggatatt gtgcgtaccg atgaccaaat cgatgtgcgg cgcccgcttg aagagcttat
      721 cctgccattc ctgggccatg cagcccgtga cggcaatgat caaatcgggg ttctgcttct
      781 tcaaatgctt gagttcgccg attttcccca gcgtcttgtc ttccgccgtt tcgcggacac
      841 aacacgtatt gatgaggatg acatcggctg tatccaaatc ttccgtattg tgatagccca
      901 gctcctccaa ctgaccggca taatgctcac tgtcagacat attcgcctga cagccataag
      961 aaataatatg ataaaatcga ttgctctgaa aatccatgta ttctctccgt ttttcacaaa
     1021 aatcataatt tagggtttac agtatataat accatatata ggccgcccct gtgaagggct
     1081 ttttaggggc tcgtggcccg caggccgccg gtcgcagttc gcaggccgcc gtgaatcctg
     1141 tgcccagccg ggatttatga cgaacgatat ctgctaactc ctgaccccta gctgctaaca
     1201 gcttgaaagg acttgtcgcc gtgcgacaag ccctttctcc ttactctctc gattctattt
     1261 tttgctcgaa gcctttattt caaagggaat ccgttgttcc cggacggttg tcttgatgaa
     1321 caaatggatg gctgccgtca tgctgatgcc ggcagcatcg caaaatgctt caaactcctt
     1381 tttgaggtct gaatcgatac gaaaagacaa tcctatctgt gcgatgatac tcccctcctc
     1441 tactgttttg cttcgggaat gacgcggact cgttcgatcg tcggtttgcc attcttatct
     1501 ttggcaatcg tatcgatgcg gatgtgtttc atttccggcg agaattcttt cgctaattta
     1561 tccagcgtgc cgtcctggct caaagccggg ttggaagcgg caccggccat gatggcctgg
     1621 tacacgatgg cagcaaattc ataacgcgtc atcatgcggt cgccgtcgaa gttcccatcg
     1681 ggataacccg tcaggatacc ggcctgtttc agcttggtaa cgtattctta agaagtatat
     1741 cttcacataa aaaaacgcag gaaccaaacc ggcgcccctt ccggtttagt tcctgcgtat
     1801 ttatagactc cactgtctaa aaggcgaatc gtgaatatac atttatcctt tatggaatta
     1861 cactatagca atatacttgc tataatagta atcatagatt ttaactcaaa ctttagtagt
     1921 tagctttaat cagcggacgc ggacgagttc gtattcccgt gtgcctaagc cgattttttc
     1981 agcgtgttcc agggtcgtct tccagtcgac attaggattg ctgtcgagga agtgatcgtg
     2041 atggtgctgc caatcgggtt tagccaggtt atccgacaac tggctgttgg gcataggagc
     2101 ggctttcatg caggcatcgg cacaagcctg gtcgagggcc accgggtcga aggaggcgaa
     2161 catgccgaac tccggcagga tagctgactc ttattcggca aggcagtcgc actaccgcca
     2221 ta
//
LOCUS       DAWCYS010000301         2211 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_6812_length_2211_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000301 DAWCYS010000000
VERSION     DAWCYS010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6812_length_2211_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          443 a          694 c          620 g          454 t
ORIGIN      
        1 acctcatgca caccgatttc ctgaccgtcg ccgacttcct gacccacggc ggcctgctca
       61 tcgcctcgct cttcgtcggc accctggcag cggcctgggt ctggcatcgc aagaaggact
      121 tcctctcggt cctggtcatc ctcagctgca tggcctatgg cttcggcctc ggcgcccatc
      181 agatccaggg ccagattcac gaccatcaga cggcctatac agtcagccag gacatccggt
      241 ccctgaacgt cccggacagc acgccgatca tcatgtatgg ctcgttcatg ccaggcctgg
      301 tctactatct ggatcgtccc atcgccgccg gcaacttcat gggcgagctg gaattcggca
      361 tccatcatac ggaccagacg aacatgtact acggcagcca ggacttgtat gacctctgga
      421 acagcgacca gccggctatc gtcgtcgtcc agccgaaata ccgcgacgaa gccctgcgcg
      481 ttttgggaac ggttccggcc cagcgcctcg acgtcgaaga ctatacggtc ctcctgaacc
      541 gggccgccgc cggaggtgac aaacgatgaa gcaaatcgac atcctcgtcc cctgttataa
      601 cgaagaagcc gtcctgcccc agttctatca gcggacgtgc gccgtcatcg acgccctgcc
      661 agactacgcc tttaccttca tcttcgtcaa cgacggcagc cgcgaccaga ccctgcccat
      721 cctgcgccag ctccacgaaa aagataagcg cgtggcctac gtatccctgt cgcgcaactt
      781 cggcaaggaa gcagccatgc tggccggcat ggactacgcc gaaggcgacg ccctggtcat
      841 catggacgcc gacctccagg acccgccgga actcctgccg gacatgatcg cctggtggga
      901 aaaaggctac cgcgacgtct cggcccgccg ccggcgccgc gaaggggaaa cgtttttcaa
      961 gaagtggtcg agccatgtct attaccgcct gctcaagaaa ttgtcccccg tcccggtcca
     1021 ggaagacgtc ggcgacttcc gcctgctcga caggcgctgc gtcaaggccc tgcgcagcct
     1081 gcgcgaatgt cagcgctata ccaagggcct gttcagctgg attggcttcg agaagaaaga
     1141 aatcctcttt gaccggccgc cccgggctgc gggcaagacc aagtggaact actggaagct
     1201 cttcaacctg gccatcgacg gcattaccag cttcacgacg gcgcctttgc gcatcgccgc
     1261 ctttaccggc tgtttcctgg ccatcgtcgc cgccatctat ctcgtcttca tcgtcctgcg
     1321 caccctactc ttcggcgaag acgtcccagg ctatccgtcc ctcatctccc tcatcctctt
     1381 catcggcggc gtccagttca ccttcatggg catcctcggc gaatacgtcg gccgcatcta
     1441 taacgaaagc aaaggccgcc cgccgtacat cgtcggcgaa agcacacggg aaaagaaata
     1501 aatcaatcaa aaatgaccgc tccgtcggca gacagatggc ggtcattttt aggttccatc
     1561 acagccattc atcactgtgg cagcagatgg atatgaggtc ctgcatgggc tgtatcaggt
     1621 atttgttttt gtgatgtact aggcggaaat ggcgcttgaa ttcgaggccc ggcaaaggga
     1681 gttcgacgag gcggcccgtt tcgatttcgt tttgaaccag gcggcgggac aggccggata
     1741 cgcctaagtt ggccatgacg gctttcttga tggcttcgga gttattgtag acgccgatga
     1801 tgcggcaggt caggtggtgg tcttccagga cgcgctcaaa gaggtcccgc gtgccgcttc
     1861 cctgttcgcg gacgaagacg ggcaggtcgt ccaggtcctg cggcgctgta atggccttac
     1921 ggctccattc gctgttcggc gaagcgatga gggtcagata atccgtaagg atgggccggg
     1981 atacgaggta tggcgaatgg actcgtcctt cgacaagggc caggtccagt ttatcttcca
     2041 tcaggagctg ttccagttcc tccgtattgt ggatagccga caggatgtcc cattcaggat
     2101 gtatctgacg gcaatgcgtc aggagttcga ccaggaagat gtcgccaatg gtgatgctga
     2161 cgcccaggcg gattggcagc tgatgctgca tggccgtcag gctgtcttca g
//
LOCUS       DAWCYS010000302         2187 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2420_length_2187_cov_5.282364, whole genome shotgun sequence.
ACCESSION   DAWCYS010000302 DAWCYS010000000
VERSION     DAWCYS010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_2187_cov_5.282364"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          455 a          634 c          567 g          531 t
ORIGIN      
        1 tcgtcgagcc gcgggcgccg gaatccatga ccgtcacatt gcggtacaag tcggtcatga
       61 aggcccgggt cttgatttca tttcccccga aatggtcaag gccccagctc caggcggcca
      121 ggaagttcca gcaggcgccg ccgctgctct tcgggtccgg cgtaatgacg ctcacgccgg
      181 gctttaccag gtcgtcccag tcgcgtatcc ctttcgggtt ccccttgcgg accaggaaga
      241 cgatggtcga cgtgtacggc gccgaatcgt tggcgaaatc cccctgccag ccttcatgga
      301 tgaggccggc cctttcgatg agcgacacat cgtgggccag ggccagggtc acgacatcgg
      361 ccttgctgcc ttcgatgacc gaccgggcct gactgcccga tccgccatgg gactggatga
      421 tgcggatatc cttgcctgtc gtctcacgat agtgctgctg gaataattca ttgtattctt
      481 tatataactc acgggttgga tcatacgaga cattggtcag cgtcatcgtg ccgccgcccc
      541 cggcgtcatc gctgcgtccg cagccagcca gggccccggc gacggcgagt gccgccgaaa
      601 aagccagtat agctatgatg cgcttcttgt tcattttctt tccccctcta taccggctta
      661 gattccttac ccctcaaata aagccgtcga taaatagcgt tcgccgccat ccggcaggag
      721 gccgacgata gtcttgcctt cgttttccgg ccggctggcc agttccatgg ccgcctgcag
      781 cgacgcaccg gacgaaatgc cgaccaaaag gccgtcggtc ttggcgactt ccctggcccg
      841 gccgtaggcg tcctcattgg atacgtcgat cacatcatct acgagagata tatccaaaac
      901 tttcgggatg aacccggcgc caatcccctg aatggcgtgc gggcccggcc ggccgccgtg
      961 caggacggcg gaatctttcg gctcgacggc gacgatctgg acgtccggct tatggccttt
     1021 catgccctgg gccgtaccgg tgatggtgcc gcctgtgccg acggacgaaa tgaagatatc
     1081 taccttgccg tccgtgtcgc gccagatttc ttccgccgtc gtctggcggt ggatggccgg
     1141 attggccgga ttgtcgaact gctggggaat gaaggcattg ccgtaagaat ccctcaattc
     1201 ttcggctttg ccgatgctgc cgcgcatccc ctcgatgccc ggtgtcagca cgatttccgc
     1261 ccccagggcc gacacgatct tacgccgttc caggctcatc gtatcgggca tgggcaagat
     1321 caggtgcagg cccttcacgg cggcgaccag ggccaggccg atgccggtat tgccgctcgt
     1381 cggttcgatg atgaccgtat ctttgttgat cttcccctgc cgtatccctt cgtcgatcat
     1441 ggctgccgct acgcggtcct tgacgctgct caaggggttc atgtactcca gcttggccag
     1501 gacgcgggcc ttgaggccgt gccgagcctg ccaatgagtc agttccaaga gcggcgtgtt
     1561 gccaatcagc tctgtcaaat acgttgccac atgctccatg tactcagctc ctttttggca
     1621 aaaagaaaag cgccgcctgc tgatgcagat ggcgctataa atagacgtat gcccatgact
     1681 tcatctgcca ggttatttcc tgctggaatt ggcaccttac ctacgtaggt tgccgcagcg
     1741 tcatcgggcc agtccctcag ctgctctgga tgaataggtt tcgtattgta ttgtccttat
     1801 gaatggttgt acaatacgat catatcatac aacacatgat ggtcaaattt tttaagtacc
     1861 ccatgataac atacctcttt gatgactaaa atgaatagct tcctatcgga aacgttacca
     1921 agagtgtaca ctttacagag tatattgtca agggatttta agcaaaaact ttcatatttt
     1981 acaatgtaaa cgttatacct atagctttag ataggaattt aactttataa attatatttt
     2041 ttataagtaa aaagggcttg ttgtatatac agcaggccct ttttagggat tagccgttag
     2101 tgtctaacag ctaaccccaa accccaaact gctaactgct aaaaactcac tgataactga
     2161 tcaactcaaa ctcctgaccg ctaacac
//
LOCUS       DAWCYS010000303         2180 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2434_length_2180_cov_4.427765, whole genome shotgun sequence.
ACCESSION   DAWCYS010000303 DAWCYS010000000
VERSION     DAWCYS010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2434_length_2180_cov_4.427765"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          405 a          652 c          596 g          527 t
ORIGIN      
        1 cactgcggcc gggagaccca gtgacgcccc gatttgcgca atcccggaat tcgcgacggc
       61 ctcgaacgtg gacgctgtcc ggctgccgtc gatagctttg acgtcgacgc cgctcggcag
      121 gagattcagt gtccccgggc cgatttctac gtgccgcagg tcgtccgggt caatctgctc
      181 gcccgggccg tatgtctcgc tcaagacatc gcccagatca ttcgtcgtac cgctcgacgt
      241 aaagaatagc gtgaaaaacg acttgatgat tgctgcggat aattccgcat tggtgtatcg
      301 gctaatttgc ttcaaaacct caatcgccgg cgccagatat ggcacgccgc gatactgttc
      361 cgggcgctct tcatgcgaaa tttgcaggac taagggccgg cctgtgcgtc ggccaaaggc
      421 ctcgacacgg gaccagcgca gggcctgcga attgttcgta gggtcatacg gcactcggtt
      481 agcaatccaa tatgccacga cggccccatc ggcatcgatt tccacgccgt taataatccg
      541 gttatttgtt ttcgggttat aacattcgac ggtcatgctg tccgtgttgc tgtaagcctg
      601 catagcgcct gggttacata cccgggacgc ctcgaacagt tggacccgtg tcgagtacag
      661 gccgcccggc gccggcttgc ggtacttgat agccgcccat gcatcgccgt ccaccaggta
      721 cgacagatag gcgatgtcct gcatgtcgta atatgaatgc tgtttatcca gctcgcactg
      781 gaccgtatcc gcccatatgc tgaattcccg ctgcgtgtgg cgctgccatg ctttagcctc
      841 gtcggcagtc atgcccagga gcttgtagtc aatcttaggg atcacctgca agcccgcgcc
      901 gatgacgttc gcccgattcg tgttgatagc gctggcccca atcggcgaat tgatatacat
      961 atccgaggac cggttgcgca gcgtgcttaa attagcatcg atgtcagcgc gcggcgacga
     1021 cttcatcggg ttgtacccgc gcagtgcctg cctggaacgg ctggcggcgc cgtcgctata
     1081 gcctgtattc gtaattttcg tattgacgcc gcctgtcggc tgccttgcgt tgcgtttctt
     1141 tcgttttttg ctcattgcag cctcctagtc acgtagtacc acctgccggg accgggatcc
     1201 cgttcgtgcg gcgccgtcaa ccgttgcgcc ggcggatatc aatctgctga tgacgctctg
     1261 tatctcggcc aaatcggcgc gggtaagcgt gcggttgccg attttatacg actgaccgcc
     1321 cgtcaggata gccgtttccg ctgcaatgta ctgttttagt cgtgcattca gtaattcgtt
     1381 catcctatcc ccctcttaat cacgccgaat cgaggccgct tacgcggacc tgttggccgt
     1441 ttttcgtctg tctgtgttcc tgtcatcagt gctttcagct tggaaaagtc cggagaaatg
     1501 gacgccaggc aggctaaatt atagactcgt aaatcgagcg gttcgtttcg atggtccttg
     1561 gcgatgttta cccagcggta tacaatgcgg ccgttcttct tttgcggttg tttttcttct
     1621 gctgtcaggc cttggaaata gatttcgtca tagccccggt tccacagcac ctcattaacc
     1681 gcatcctgct ctggcttatc gagcggaaaa tggaaatacc gcggccccgg cacatcgatg
     1741 gccaggcggt ccataatata ctgcttgccg ctgtccgtgc cgatgagcac cagcggaata
     1801 gtccgcccgt gataggcctg ggccttgctg tacttatgga taatcggcac gcccatcgtc
     1861 gacgagccgc ggacagcaaa gcgctgccgc gccaaatgca tgaggctgta gttataaacc
     1921 tcgctagtgt agtgaccgcc ggagtcgatg aacgtccggg ccaccagcag ccctgtaccg
     1981 tcggcaaagt ggtattcgcg gtcaagctgt tcgtccaact gccgccatac cgccgccgta
     2041 tccggcgcgc ctaagataat gcccttcttg atgccccagc actcctcctc ttcgccccat
     2101 ccgacgattt catattcgag gcggttatcc tggacatcga cggccgctgt cagaatcagc
     2161 acgccgtcgg gaagttccgc
//
LOCUS       DAWCYS010000304         2169 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2447_length_2169_cov_5.284768, whole genome shotgun sequence.
ACCESSION   DAWCYS010000304 DAWCYS010000000
VERSION     DAWCYS010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_2169_cov_5.284768"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          550 c          656 g          534 t
ORIGIN      
        1 ggccttgccg atgaccagac cgcttgggaa ggacccggcg atataggcca gtaccaggca
       61 gaggattccg agcatcataa gctttcaccg tccttcttcc cgcgcaggat gagccggatc
      121 ggcgtccctt caaagccgaa ggtttcgcgc aggcggtttt ccagaaagcg caggtacgag
      181 aaatgcatga gctgcggttc gttgacaaag aggatgaacg tcggcggctg gaccgaagcc
      241 tgggtcatgt agtaaatctt gagcagcttg ccgcccttgg ccggcggcgg attggtcagc
      301 tgggcatctt cgatgagttc gttgaggacg ctcgtcgaca cgcgcatgtg ctgctgttcc
      361 gacacgaact tgaccatgtc ggccaggcgg ttgatgcgct gcttggtcag ggccgaagcg
      421 aagaggatgg gcgcatactg gaggaaagcc agttcgcggc ggatgtcttc ttcaaatttg
      481 tggctcgtct tgctgtcctt ttcgacgagg tcccacttgt tgacgacgat gatgcagccc
      541 ttgccggctt catggacgta accggcgatc ttcgtatcct gttcggtcac gccttctgtg
      601 gcatcgatga ccaggacggc gatatccgaa cggtcgacgg cgcgcagggc ccggacgacg
      661 ctatagcgtt cgacaggcat gtcgatcttg gccttgcggc gcataccggc cgtatcgatg
      721 aggacgaaat ccgtcccttc ataggaccag tgggtatcga tggaatcgcg cgtcgtcccc
      781 ggcacgttgc tgacgatgac ccgttcctgc cccaggaggg cattggtcag ggatgacttg
      841 ccgacattcg gccggccgac gagggcgatg tggatggctt cgtcgtcatc gtccatggcc
      901 gggactttct tgatgtactt caggacttcg tcgaggaggt cgccgaggtt catcaggttg
      961 acagccgaga cgccgatggg gtcgcccagg ccgaggctgt aaaattcgta gatattcatt
     1021 tcttgttcga cgctgtcgat cttgttgacg accaggatga ccggcttgcc gcatgagcgg
     1081 aggaggtggg cgatttcttc atcctgaggc tggacgccga ctttgccgtc gacgacatag
     1141 aggatgacgt cggcttcgcg gatggccagt tcagcctgat aacgcatact ggtaaagata
     1201 tggttatctg aatcgacgaa ttcgatgccg cccgtatcga tcatggtgaa ttcctggccg
     1261 agccattcgg cgtcgaaata aatgcggtcg cgcgtcacgc cgggaatgtc ttcgacgatg
     1321 gaaatgcgct tcttgacaat ggcattgaag agcgtcgatt tgccgacgtt cggacgcccg
     1381 acgacagcga ctaagggttt gtacatggca gaatcctttc tatagacgaa aaaaggataa
     1441 ccggtagagc ccggctatcc cctttttgtg aaaccttagt tgttttcttc ttctttatcc
     1501 tggttatcga gatagttgcg aacttcctgt tcttcttcat ctttctgaat ggccaagacg
     1561 ctgagagaaa tctttttctt atccgtatcg atgccgatga tcttgacgtt catgacatcg
     1621 ccgatgttga ccaggtcttt gacagcaacg ccgcgttttt ccgtcaattc ggaaatgtgg
     1681 agcaagccct gcaattccgg ctcgatggcc aggatagcgc cgaagggcag gagcttcttg
     1741 acttcgcctt tgacgatatc gccgacttca aatttttcga tagcagcgat ccaggggttc
     1801 ttctggagtg ctttcaagga caaggaaata cggttccgtt ctttatcgaa tttctgaacg
     1861 aggacctgga gtttttcacc cgtatggaga acggcatcga cagagctgat gcgtttccag
     1921 gaaatgtcgg aaatgtggag caggccttct acgccgccga gatcgatgaa tgcaccatag
     1981 ggcatgatct tgacgaccgt gccttcgagg acagcgcctt cttcaatgtg tttgagggct
     2041 tcttcgcgtt ttgcttcgcg ttctgcttcg aggacggctt tgcgggacag ggcgcggggg
     2101 gtctggggtt cgtcgatgtc gatgactttg agctggcggg tctggcctcg gagctagtcg
     2161 aaggctggt
//
LOCUS       DAWCYS010000305         2164 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2456_length_2164_cov_3.710763, whole genome shotgun sequence.
ACCESSION   DAWCYS010000305 DAWCYS010000000
VERSION     DAWCYS010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2456_length_2164_cov_3.710763"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          575 a          541 c          587 g          461 t
ORIGIN      
        1 gaagcgcatt gccgaaatgc aggcatacgt aaaagggaaa ccggaccatt tcacattgtg
       61 ccagtccgtc atcgggcagc gcatcagctt caagactgct tacgaaacag ggcagtcggt
      121 cgtagaactt catgagggag cccggacagc gaagctggcc cgcgcctata tgcacagtgc
      181 tgatgaaatg gtggcgctgt gtgatgagat ccgaagcgcg ttagggaagg gggtatgatg
      241 atgcctttca tcgaagaatt gccgaaaaag aagatccagg acttttttgc ggaaggggat
      301 ccggaacgga cgagccaaaa atcgcatacc aagcaggcgg aaaagaagac ccgtattccc
      361 gtatccaagc agaaccgcaa gaaaatcgtg acctacctgg acgatgaaac gtatgatttc
      421 ttcaagcgct attgtagcga acatgacatg aaaccaagcc tggttgtgcg gcatctcatc
      481 aaagaatgtc tgaacaagcc tttgtaagtt ataagttatc ggaagaaggt atatatcatg
      541 aacaaaccca tcaaattctg cctcatcctc accctaaccc tatgtaccct cctgctatca
      601 ggctgtggtg aaaaagacaa attcgacaaa gaccatgctg aagtcatcaa actgatggaa
      661 caggcgaaag ctgtacagac caaagacttg aagatacggg acaacgatga cctgacggcc
      721 aatcaggacg acgtcaaagc cgtagaagct gacatcgcgg atacagaaaa gcgccatcag
      781 gacatcatcg accaggtcaa tgccaaactc caggaaatgc agggctatgc cgataaggaa
      841 gcgtccctgg caccacgcct ggagaacatc aagaaagaag tcaaggaaca ggatcagcag
      901 tggcatgacg gcttgaacga atacaaagcc atcacggata tacagaaaca ttctgccggt
      961 gccaaaccga catacgaccc gttcaagggc catctgcact ttgaaaattg accacataca
     1021 gcaactgccg tacctcatag ggtgcggcag tttttttatc actcatcgcc ggtatcctgc
     1081 gtcgaaagga agccgaagtt ctctaatttc ggcgcctgtt cccggtcgtt gtctgacgga
     1141 tggtacgggt catactcata gaacatgccg aactgtgctt catcatgctc gaagcgttcc
     1201 tgtgcatatt cccgttcatc agcttcaatc tgacggatcg tctcagccgc aatcatgcgg
     1261 gtctcatcat tttcgttgag tgtcgccagc gaggccaggg ccgcggattt ctgtgtcgta
     1321 ccgacgatac cattgttatc ggcatcgagg atcgatgctg ctgtgcccag ccgctccccg
     1381 gacagggcag ccgtcgtctg agcctgtacc agggcgtcgg cattggcata ggtgacttca
     1441 ttgagcaggc cccgctggaa gccgttcgct tccatgggat cggttatctg ctctggcgtc
     1501 gcagctgtcc ggagaatact gtcatgaagc atggccgtcg ggtcgaagac gaagctgtgg
     1561 ccatggaaat tctggctgta gcggtcgatg gctgctgaaa tgcgctgatt caggttcttg
     1621 acgccggagt agagcagaat cttattcttc aatttctcca ggtcctgctg atattctgcc
     1681 agcttttgag ctgtttcctt gatgctgtcc tgcagtcgct gcatgtcgat gacgtgatcg
     1741 ccaggatgga tgagctgatg gtgccgggcc tgagccctgg gcatcccggc aaacatggcc
     1801 acgaccatag ccgtcctcat gacgaggacg gcttttgtgc ttaccatgaa aaacacctcc
     1861 catcataaga actgatacaa gaagtcatca atggcgtcgt aattaatgag aaggaagacc
     1921 ttccgttcga cgtgggatga aacgataggc ccgaagtacc gggagtccaa ggaatgaatc
     1981 ggtggattca ggaagagcat cgtcccttca ggaacggaaa acgtaccatg cggcagctga
     2041 ggcagccatg gccgatgata aatcggatat acctggccgt tggtggccat gacgtcgtcc
     2101 gtcacggtat acgtgtcgcc gggaaaagcg gcgacacgtt tgaggagacg gaaatcatag
     2161 ggaa
//
LOCUS       DAWCYS010000306         2152 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2470_length_2152_cov_5.489747, whole genome shotgun sequence.
ACCESSION   DAWCYS010000306 DAWCYS010000000
VERSION     DAWCYS010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_2152_cov_5.489747"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          428 a          542 c          625 g          557 t
ORIGIN      
        1 gacggaacgg tcccggcttt cccgccggca tccactgcgg ctgcagtggc agtcccggtc
       61 aggggctttt ccggcttttc agctggtgcc gcccccgttg ccggccccca ggccaggcag
      121 gcagcgaaga ccagcgcagc catggctgta catttcatag cgttcctcct tcgtaggatt
      181 gagaaaacgg agctgtccaa tgaagacagc tccgttttac gttggtcgca ggccgttcat
      241 cgtttgtcgc aagggagcaa aaataacaag ccatcgcgat gaacgatgaa cgacgaacga
      301 aaaactttta agtcaaagga atcgtgcatg atggcagaaa ccttcatgga accgtcctta
      361 tcttatttat gctgttccgg gcggctcaac gggaagagga gaacgtcgcg gatagacggg
      421 ctgttggtca ggaacatgac cagtcggtca atgccgatgc ccaggccgcc tgtcggaggc
      481 aggccatatt ccagtgcgcg gcagtagtct tcgtccatct gatgcgcttc gtcatcgccg
      541 cgttcccgtt ccttcatctg ttcgatgaag cggttgcgct ggtcgatggg atcattcaat
      601 tccgagaagc cattggccag ttcgcggcca tagatgaagg cttcaaaacg ataggtcaag
      661 agcggattgt cccggtcctg tttggtgagc ggcgagattt ccaggggatg accggtgatg
      721 atcgtcggct ggatgaggtt ttcttcgacg aatttttcaa agaaagcgtt gatgatcttg
      781 ccgacgccat cgtactggcc gtattcaacg tcgtcttcgt cagccatctt gcgggccgtt
      841 tcgacatcgg taatgccttc aaagtcagca ccgctgaatt tcttgacggc ttcgatcatg
      901 gtcatgcggt cccacggcgg cgtgaggtcg atttccttgt cgatataggt caatttcatc
      961 gtgccataga gcatttctgc cagtttggag accatgtctt ccgtaatgtt gatgacgtct
     1021 tccaagtcgc cataagcctg gtacaattcg accgtcgtga attccgggtt atgccgtgta
     1081 tcgatgcctt cgttgcggaa gcagcggttc atttcataga cccgttccaa gccgccgacg
     1141 aggaggcgtt tgaggtggag ttccggtgcg atacgcaggt agatgtccat atcgagggcg
     1201 ttgtgatggg tcttgaacgg acgggctgca gcgccgccgg cgatggtatg gagcatcggc
     1261 gtttcgactt ccaggaagtg gcggtcatcg aggtatttgc ggatgccctt gatgatcttc
     1321 gaccgtttga tgaatgtgtc tttgacttcc ggattgacga tgaggtcgag gtagcgctgg
     1381 cgataccgcg tttccttatc ggtcaggcca tggaattttt ccggcagcgg acggagcgat
     1441 ttggacaaga gggtccattt ttcgacgcga atcgtcgttt cgcccgtatg ggtgacgaag
     1501 actgtccctt cgacgccgac gatatcaccg atatcgagga gcttgaataa attgtacgat
     1561 tcttcaccga gtacgtcttt gcggaaatag agctggattt cgccgctcaa gtcgcggagg
     1621 acgcagaatg cagtcttgcc gtggcggcgg atggccatga ggcggccggc gacgctgacg
     1681 acggtttcat tcttttccag ttcaacggcg tcatctttga cttcctgtgc atgatgatcc
     1741 cagttgtatt tctgaccgaa gggatagacg ccggcgtcga tgaattcctg catcttttcg
     1801 cggcggacct tcatttggtc attcaatttt tcttcttcaa taatgggcgt ttctttttct
     1861 ttcgtgtccg acatgatatt cctcctagga tttattctgc atcaggcggc aagatgcggt
     1921 atttgatttc acctgccggt gctttgacgg taacgatagc cggattggca gcatcgatga
     1981 cctggccgag gatggctttc cctacaggag attcattgga aatgcggccg ttgaacggat
     2041 cggcttctgc cgtaccgacg acggtgtaat cgaagcggtc ccccgtttcg gcatcttcca
     2101 ggatgacatt ggaaccgagg cggactttat ccgagccagc atcggttgcc gt
//
LOCUS       DAWCYS010000307         2149 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_6388_length_2149_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000307 DAWCYS010000000
VERSION     DAWCYS010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6388_length_2149_cov_7.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          455 a          654 c          621 g          419 t
ORIGIN      
        1 cattccggaa atcgaaccgt cggcggtcct ggccatgctg cgcatctccc aggccgctga
       61 aatcatccgc tcgtccatca acgacgtcct ggccaagcaa taccacttgt cccaggggaa
      121 actgcgggtc atgatcgtcc tgcaccagag cccggaaggt attgcaccgt cacagctggc
      181 tgagaagacc ggcgtgaccc gggcgacgat tacgaccatg gtcaagcgca tggtccgcga
      241 cggattgacg acggtcgtca tcgatcagga agacaagcgg ggcaaaaagg tccgcctcac
      301 ggcccagggc cgccagttca tggaccaagt actgcccggc cattatctgc gcatttcgac
      361 gctcatgaac cgcctcagtg aggacgaaca ggccgaactc atccgcctgc tgacgaaact
      421 gagccagaaa tgattttctc agcaaatttt aacctgaaaa gcactgcttt tcaggttttt
      481 ttcatttttc cctgttacac tgggggcaaa tggaaaaaga aagaaggttt gcccatgtat
      541 ccgactcatc acttccgcca ggacttccat tacttgtccc tgcgctcatc tctgttggaa
      601 gaaatctctc tgctctatgg cctgcccctg gggcaggcga agggccaggc cggccggatc
      661 tgccgctgcc gccgtatgcg ccgggaactg ctcgcggcct gccgacagca gccggaccag
      721 ccggtatatc cctatcttct gggcgtcctc ctggaacggg tcggtcaatg gccgctgacc
      781 agccgccccc gatgggccta tgaccaggcc gagggctatt acgaccgggc ccggcagctc
      841 cagcagcggc agcctcccgg cacgtatgac cgccagcact atctgcgccc gtctgtggcc
      901 ctgctgcgcc tgagcctgcg ccgcagacag gaggagcggt tctatgactg gtgggaccgc
      961 tgcggcggcc tgcgccgctt tcaccgcgat gtacaggccc tgttccaggt gcgctggctc
     1021 atcgtcaagg aagactatga acgggcggcc tttaccctcc ggaatctgca tggattgacc
     1081 ggcaggcata gcgctttttc agctgcccag gcccgaatcc tatcagatat cgtcacggcg
     1141 gcctgtcagg ggccgggggc gatactcaag ggaacctatg gcccgtatgt ccgccaggcc
     1201 ctgtgggacg ccctttttcc tgccaggccg cgacaaatga cggactccgt gttataatga
     1261 caggcatagg catatttgcc gggaaatgag gaactgctat ggaaatcaag atcatcccgc
     1321 tggggagcga cgacaagccc ctggattcgt atggccagga cggccagtct tttgccgaga
     1381 cttatgcgcc catcgttgag ctgctgctgc gcgaaagccg cctccatgcc gtcgtccata
     1441 ccgtcccggg tggaccggcg atggcccagg ccgtgtgccg tctcatcgaa gaggcccgga
     1501 agcggcaggg ggacggtgct gaggccatgc ccttcgacgt aacgcccatc gtcgacgtga
     1561 cggcgcgcct gcgccgcgaa gacggctgcc cctgggaccg ggcccagacc catcggacct
     1621 tgcgccgcta cctcatcgaa gaggtctatg aaatgctcga tgccatcgac agccacgacg
     1681 tagcgggcat ccgcgaggaa ttgggggaca tcctctatca ggtcgccatc cacgcccgca
     1741 tcgcccagga aaaaggcctt ttttcggctc aagatgtcgt aaaagacgtc acagataaga
     1801 tgattcggcg ccatccctac gtttttagcc aaaaaagcct tgaaaacata ggtacgagta
     1861 tgttaaactg ggatagatta aagcaaggcg aacaacgcca gcagcatgct cacctgctgg
     1921 acggcgtcgt tcccggactg ccgtcgctcc tggctgcaga taaacttcag gaaaaagcgg
     1981 ctaagaccgg tttcgactgg cccgatgtcc agggcgtctg ggataagttc catgaagaat
     2041 gggaggaatt ccgtcaggcc cttggcgaag gagattccgt ccacgccgaa gaagaagctg
     2101 gggatgtcct gtttgtcttt gcaaatttat gccgacacta tcatataga
//
LOCUS       DAWCYS010000308         2148 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2476_length_2148_cov_4.781175, whole genome shotgun sequence.
ACCESSION   DAWCYS010000308 DAWCYS010000000
VERSION     DAWCYS010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2476_length_2148_cov_4.781175"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          393 a          565 c          677 g          513 t
ORIGIN      
        1 aaaggtcttg ggcttggtgt cgacgagcat ctggcggacg aaggacgtgc cgaattccgg
       61 gatgcccagg ctgccgactt tcgagcccag ttcttccggc ggcacgccca gggccttggt
      121 cgagctgaac aggctcagcg tcgccgggtc atcaaaggga atagtcgtcg ccttgatgcc
      181 ggtcatatct tcgagcatgc ggatgaccgt cggatcatca tggccgagga tatcgagctt
      241 gaccagacgg ccttcgatgg agtgataatc gaaatgggtc gtaatgatgc cgctgtcctt
      301 cttgttggcc ggatactgga cgggcgtgaa atgatggacg tccatatcgc ggggaatgac
      361 catgacgccg cccggatgct ggcccgtcgt attcttgatg cccgtgcagc cggcgaccag
      421 gctgttgaca taggcatcgt tgaccttgat gcccttggct tcggcccact tcatgacgta
      481 gccataagcc gtcttgtcct tgacggcacc gatggtcccg gcgcggaaga cgttgtcctt
      541 gccgaagagt tcttccgtgt acttatgggc tttcggatgg tattcgccgg agaaattgag
      601 gtcgatatca gggaccttgt cgccgtcgaa gcccaggaag atagcgaacg ggatgttgtg
      661 gccgttcttg tggagcggcg tgccgcactt cgggcagacc ttgtcgggca ggtcgaagcc
      721 gccgccgact tcgccgtgcg taaagaactc gctccactgg cacttggggc agacataatg
      781 cggcggcagg gggttgactt cggtaatgtc cgacatagtc gccacgaagg acgagccgac
      841 agacccacgg gagccgacga ggtagccgtc gtcgttggag tgcttgacca gcttgtgggc
      901 gatgtaatag aggacgccga agccgtggcc caggatggac gtgaattctt cttccaatcg
      961 gtcttcgaca atcttcggca gcgggtcgcc gtagatttca tgggcctttt tatagcacat
     1021 gttcttcagt gcttccgacg aaccggcgat ctgcgggaaa tagagcgttt ccttcggcac
     1081 cggccggcag tcgtcgatca tatcgttgat cttattgggg ttggtaatga ccacttcccg
     1141 cgcccgttcc tcgccgaggt aggcaaattc agccagcatc tcgtcggtcg tgcgcaggta
     1201 gaggtcgggc tggaattccg catccttgaa gcccttgcca gtcatgagga tttcccggta
     1261 aatcttgtct tcggggttca tgaaatgagc atcgcaggtg gcgacggtca atttccccag
     1321 gtcatcgccg agcttgagga tcgtccggtt gatgtcgcgc aggccttctt catcggcgac
     1381 gaagccttcg cggacgagga agccgttgtt ggtgagcggc tggatttcca gatagtcgta
     1441 gaacgatgcg attttcttca attcttcata aggcagcttc ttctggacca tcgagcggac
     1501 cagctcgccg gcttcgcaag ccgagcccag gatgaggcct tcgcggtatt ctgacaggac
     1561 cgcccggggg atgcgcggcc ggccgcggta gatatatttc aaatgggaaa tgctgaccaa
     1621 tttatagaga ttgcggatgc cgatctggtt cttggccagg atgatgatat ggtacgactg
     1681 gtcgatctta tcatcgaaga ggtagccttc catgccatag atgaccttga cgttgttggt
     1741 cttgtccgtc gagatttcct ggagcagcgg gaaggactgg acgacgccgt ggtcggtgac
     1801 ggcaacggcc ggatggcccc acttcttgat ggtcttgatg agctgtttga ccgtgatcag
     1861 ggcgtccatg tcgctcatga ccgtatgcag gtggagttcg acgcgcggcg tgggattatg
     1921 gtcttcccgt tccactgtac cgccgtcggc ttccatcatg tcgtaggcca tgaggacgta
     1981 gtcgttctgg taattgtcgt actggatatt gccgtggacc cggacgtaga cgccgggctt
     2041 gagcttcttg accaggttgt cgtattcttc cggcgtcagc gtgttcttgc ggcggaactg
     2101 ggcgcctccg ccctgcttgc tgccgtcgag gaacttcttg gctgcaat
//
LOCUS       DAWCYS010000309         2141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2479_length_2141_cov_6.824065, whole genome shotgun sequence.
ACCESSION   DAWCYS010000309 DAWCYS010000000
VERSION     DAWCYS010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2479_length_2141_cov_6.824065"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          488 a          542 c          581 g          530 t
ORIGIN      
        1 cagcggcggt accaggcgca ggacgagttc gccgatagct gtcgacagca tgtgtttgtc
       61 gaagcatttg tgcttcaagt cgacgctgtt gatcttgtcg tcgaattcaa gccccaggaa
      121 gcagccctga ccgcgggctt ccttcagatg cggcagtttt tcgcgggcct gttccaggaa
      181 gtatgccccc atcttcttgg cattgccggc caggtctttg tccaacagtt cgttgacttc
      241 ggccaaagca gcggcgcagc agaccggatg gccgccgaag gtactgccgt gggaacccat
      301 ggtgaaggct ttggaaacgt cttcgcgggc gcagatggct gcaatcggca tgccgccgcc
      361 aagggctttt gccatggaaa cgatatccgg tttgatgccg tagttcatgt aggacatgac
      421 agcaccggtg cggcaccagc cgctctgtac ttcgtccagg aggaggagca ggcctttttc
      481 atcgcacagt ttgcgcaggc ctgtgaggaa ttctttcgtt gccggatgga caccgccttc
      541 accctgtacc ggttcgacca tgatgccgat ggtgttttcc gtgatggcgt cttcaaaggc
      601 tttcaaattg ttgaattcag catatttgaa gcccggtgtc atggcgccga aaccgatctg
      661 gcaggcattg tccggctggc ccgtagcact catggaacca aaggtacggc cgtggaagga
      721 ttcttttgcc gtaatgattt cgtatttgtt gggaccaaag tgttcgacgc cgtatttacg
      781 ggccatctta atcatggctt cgttggcttc tgcaccggag ttctggaaga aaatcttgtc
      841 catgccgatg gtcgtgcaga ttttttcagc cagcagggcc tgcgggaccg tgtacggata
      901 gttgaaggtc tgcatgatgt ccatgcactg gtcgatgacg gccaggacga ctttatcgtt
      961 acagctgccg gcactgttga cggcgatacc agcgtagaaa tcgaggtacg gcgtgccatt
     1021 ttcatcgtag acgtacatgc ccttgccgcg ttcagcgatc atgtcgaagc gttcgtacgt
     1081 ttcgatcatg tacttgtcta ctttggcttt gatttcttct ttggtcaatc ctacatcgcg
     1141 taatctcatt cgtaactccc cctaaactga tgattttata aagaaatgat aactaacaaa
     1201 gaattcccca aacaaaaaca gaggcaagga aagctgatgt tccatcaggc tcccttgcct
     1261 cttgtctccg ttattttctt tgttgaatat agtgtaatac tttacacttt ctttgtcaat
     1321 atgttatttg cttttatttt tataaatctg tgttatttta actttctaat tttaaaggca
     1381 atctttgaga aggcatttac aatttgaccg tcttcagttc actcgacggc agggccgctt
     1441 ccctgacttc cgtatccatg gcgacgtcga tatgggtagc gaagcattcg ccgtatttgg
     1501 ccttgcagta tacaccgaag atgacgccca gcagggacca gccggctacc atgccccatt
     1561 cctgcggagc cagggcggca ccggaaccgg ggacgatgta catgacgacc atgaaggccg
     1621 acatgaggac agccatggca ccgacgaatt tatagtggcg taccttatag ggacggacca
     1681 tgtccggcgc cttcttgcgc aggatcagga aagaaatgga aaccatgcag taagccatgc
     1741 agcagccgaa gttgccggca tcgacgaccc agaccagcat cttgcggccg aagaacaggg
     1801 cgacaatgga caagacgccg atgagcagga gcgcgttgac cggtgtctta tatttgtcgt
     1861 gcaatttagc aaaagtatgc ggaatcatgt acgattcagc catggaatac atggcacggc
     1921 tgccaccgat gaggaaggaa ttccagctgg tgacgacgcc gcagataccg ccgatgatga
     1981 ggactttggc catcatggaa ctgccaaagg cgacagccat ggcatcggcc gtgaccaggc
     2041 tgccgacgcc tttcatagcc agggctattt cactctggtt gaggatatag ccgacaccga
     2101 agatgatgag ggcatagaag agaaccgcca ggacgatgga c
//
LOCUS       DAWCYS010000310         2132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2495_length_2132_cov_5.262879, whole genome shotgun sequence.
ACCESSION   DAWCYS010000310 DAWCYS010000000
VERSION     DAWCYS010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2495_length_2132_cov_5.262879"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          412 a          568 c          610 g          542 t
ORIGIN      
        1 ggtcagcacc tgacgggcca aaaagggcct atcaggggat gcaatggctc ccatcatagg
       61 ggagctgtca gcaaagctga ctgagaggtt tacgcgctct tacgccgtga tttcgttttc
      121 aacgacttca tggatacccg tgatggggtc ttcataaagc gtaccgatgg cgttgacggc
      181 ggtgacgacg tttgccggtt cgacgccggt gacgaggtta cgcatctgta cggatttttc
      241 gcacaaattg tcattttcgt cgttgtagct gatgtccagc tttaacttgc tcgaaatcgc
      301 tttgcgttct gctgccatag ttcatttctc ctttcttttc gtatatgaag agccttcgta
      361 gctcataaaa gcctgccatt atacctggat aggctgtggt tgaatctctc cagcgggctg
      421 ccctttggga cagcccctac gcacaaggcc gatggccgtg aatttttttg gtccgcagga
      481 ccgcggcgaa accgcatccg taggggcgcc acgtgccttt tcagcccgtc aggtcatgac
      541 tgcaaagaat gtcaagacgg ttgctgaaac ggaccgccgt agagggggcg cccgctacgc
      601 gctgagggct tcccgaagcg atgttgttag tttgagtctg acgccgcgca gcggcatttg
      661 agtccgaggc ggccggccac ctgcgaactg cggcctgcgt tcatcatgcc gcctgagcgc
      721 catgcatctg gtgcgggtac tgtatgatgc ccgtcgcgat ggcccgggct gcttcgtcgc
      781 ggtgggcgtc gtagcggcgc cagtcgaagt cgttgtcgat gaaacagact tcgacgagga
      841 cggcaggcat gatggtattg cgcaggacaa agcgtcccgg ccgttccttg agtccccggt
      901 tgggcagatt caggacggct ttcaagttgg tctggatgca gtgccccagc atgaggctgc
      961 cggtggaccc ggaaataagc gtttccgtgc cgctggccct gccgttatag gcgttgaaat
     1021 gaatggatac gaagagatca cattcggcct gattggcctg gtcgcagata cgatagaggt
     1081 catcgtcctg cccaaagaca acggctattc catttcgttc taaatattgc tggacaccca
     1141 gggccaattc ataggccgca tcacattcac acagattcag gcggcgattg acggcgcccg
     1201 gatccagctg ccggtcgtgc ccggcattga gatatacttt catagcactc ctttcgatag
     1261 tatggcctga cggccgtttt gttgttcatc gtaggtcgtt cgtcgttcat cgagtttgag
     1321 gcttaacggc cccgccggtc gcaggccgct tcggaaaaag ccgtagctcg ttgttcgtgg
     1381 ttcgttgttc gtaaaaacct gccattatac ctggataggc tgttgttaac tctctctagc
     1441 gggcttccca cgcggaagcc cctacgcaca aagccatcgc cgtacatttt ctttgcgaat
     1501 tgcgaactac gttgattttt tatcgcctgt atgcgatatg taggggcgtg cacgtggcgc
     1561 gcccgccgtg acctctgtgc atagccggaa ttcatgacga atggcacatg gtcgtaatca
     1621 aatcatgagt ctgtcgcggc ggaacgccgc atctgattcc gaggcggcct gccgcctgcg
     1681 acctgcggcc tgcgtctccc atttactgcc tcatatggac gctggcggtc ttgccgccga
     1741 tggcgatgcc ctgcatgggc ctgtgtttta tggccggaca ggggcagtgc ccgcgtccgc
     1801 agtctggata gggcctaatc ggcagcaata cggcactggc tctctgcaag atgaaaatga
     1861 gcttcctttt atcgaggttc ctcaaaaatc gtagtatccg gttcattctc gtctcctttc
     1921 tcttattgga ttttagacat atacagcgta atgacgccgc gtttatcgag tttcatataa
     1981 gtcatatagc cgtctttccg ttccaagtct ttctgccggt cggtcataca gttagagatt
     2041 tccaactggt tctgttcgat gacggacagg ctgatttccg ggaaaaagct gcgggcgaat
     2101 catgagcggg gaacctggga tgttgacttc gg
//
LOCUS       DAWCYS010000311         2129 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2500_length_2129_cov_6.296528, whole genome shotgun sequence.
ACCESSION   DAWCYS010000311 DAWCYS010000000
VERSION     DAWCYS010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2500_length_2129_cov_6.296528"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          500 c          561 g          651 t
ORIGIN      
        1 gcataatgtc gccgttctca tctaattcac atcgcgacga atatgtcggg ctgatgcatg
       61 gcattaagtt tcccgtttcg tctatttcga agaagttttc accgtcaaga gtaattttta
      121 cccagtttgt tgagtcggtt ggtgtttcgc cagcaacatc agtgccaatg caacgatatg
      181 tatttccgtc tgtgtaggca acgacagttg gatagctgta agttgttgac tcattccagg
      241 ccggagctgt agcggcaaat gaagtagctg aatagtttcc cgcttgttca gcccggtttg
      301 tggcgatgtt tgcctgtgct gttgctttgt tggcttcttc tgttgctgcc gcgtgttcgg
      361 cagcggcttt ttgcatgtga tccagtgctt cataggcata tgtcatagcc agggccgctt
      421 gttttgttgc ttcttgtgcg ctgattggag ccaggttggc gcgataaatt gtttcctgaa
      481 gcatcatgat aatccagtcc atcccttttt cgatgaggga aaaaggtaag cggtccggca
      541 ggtcgatgtt gttttgcagc ggtgtttctc tggttatttt gatacgcgta tcggcgtcta
      601 tggcgtcgcc cgctactgga taggtatatt tgttttcgac ggtatcgaat tggtagtttg
      661 tcgtgatttc tgtttcgttg ccgacggtat ctactaagta gccatgtata tcacctccaa
      721 tccggtatgg gtatgggtag gaaaacgttg tcgtttcccc atcgccacga tacgttattg
      781 atacttctgt tgcttgtatc attttttagc ctcctttgct tttttctttt cttcctgggc
      841 gcgctcttta gccgttttat atcgccggtc gaagatgacg gcattggcca gggccgtgag
      901 actgcggtcg gtgtctacca tgctgaagcg catgagggac cagaagccat cggtcagggt
      961 gtccggcaat ccaacaaagc ggttcaaggc gcggtttgcg gcgcgggcta catcggtggc
     1021 gtcctgattc ttggatgctg ctgctttcga ggctttcatg agttcgtcta cagcagatac
     1081 ggccaggacg ttgctactgt cgtagttcgg caatccgaac atatgatttc cgatgatttc
     1141 ggccgcatcg cggacgacgg ggataccttg tacagtgttg gtcatggatt tgacgcccat
     1201 ttttttgagc agtttgtcca ggtcatcccc ggatacggcc gagcgataca gctgttcaaa
     1261 tacgctgttc aggacaatcc agtacagcat tgcgttgaac atggccaggc gattgcccga
     1321 cttccactta tagcccgcgt cgatgagggc attcataacg gtattacagt atgagtagaa
     1381 gggcgtaatc tgcgcgacga gtccattttt tcgttgcagt tcggcctggt cttttaccat
     1441 accggagccg agcacgtcgc gaacggcctg gtctgcttca aagagtgcct ggtcgcgcat
     1501 caatttttcg tccgtcttcc cagcgtcgat ttgctgccga agggattctt catactgata
     1561 tttccacaat gccaggctgc acatgaggtc tgtttcggtg atgaagaagt agccgtagcg
     1621 gttgagtgcg tcgcgagcga tacgtgcctt ctgccctgcc cggctggtgt ttttcggcat
     1681 agtcaggcgc attttctgct gcatgtcttt atctatggtg ttgatacggt ccgccatgaa
     1741 aggcgagtgt tccatgacaa agcggcgatt ccgattgtat gtcggcgtcc ctttataaaa
     1801 tccaatgcca aaattcacca tggcttttac tgtattccat accccaatgc ggttcatcat
     1861 cggcaggata ttcaagccat tcaagacagc tgtactggtt cgataggcca taaccgcaaa
     1921 ggtcgtgtta cgacgcatgt tttccagcat acgggatatt ttatcggtct tctggacgtc
     1981 ggtcttccaa cagtcttttg cccattgttt aagggacgcg tacgttttca tgccatagtt
     2041 ttcctgaaca gccgcttcca cgtccggatg tgaaattaat ttgtacacat cagtgacggc
     2101 ttcgcgcata cagatatggt ggattgctt
//
LOCUS       DAWCYS010000312         2125 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2502_length_2125_cov_4.486473, whole genome shotgun sequence.
ACCESSION   DAWCYS010000312 DAWCYS010000000
VERSION     DAWCYS010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_2125_cov_4.486473"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          572 a          514 c          527 g          512 t
ORIGIN      
        1 gtccattgta gatacaccgt cgaactttgt cacgccgaca cctttcagct gtgtgagtac
       61 ggcgcctgcc tggtccttgg ttaatcccgt agcgtcggca atatcactcg tgggatcact
      121 gccgcagccc gacaccagca gggccgacaa agctaaagag gctagaattg attttttcat
      181 tacgtcatct cctctttttg tatatactca tccggcaatg aaaaaccgga agtcatctag
      241 atcagcatcc gcgcaggcct ggccatgcag gagcttttcc agcatgtccg cctggacgtc
      301 tgcgctaaag tcgttgcctt taatgtgcat cagctcatga aggacagtgg ctttttgtgt
      361 ttcccaatta caacgggcat tgatgataat tgtatagctg ccgtcgtcat tcatgtggac
      421 caatgcccgg attcgcccgg gcaggtcctg ataggtcagt acaatattca ctttttacct
      481 tctttcgctt tcaatccatt aatcagattc agtacgatat cgatgtcggc tttactcagg
      541 tcctttgtcg cgtcgaaaag gatgcggcga ttagggtccg ttcgcagctc ttccgcgagg
      601 gccgcgactt ccggatccac gtaatagccg cggctgtcat cagctgtttt tgggtcctca
      661 tcagggccta gcaaatctag gggcgatatg tggagggcat ctgcaagcag cgccatgtta
      721 tctcggcgca tgttctttat gtatccggat tcccatttct taacggtact cttgctaacg
      781 ccgactttat cggctacttc ttccaaggtc aaacctagtt cttttcgcct gttagctatt
      841 aattcattaa ctttcatatc gcgatctctc ctttttatcc tacatatata atactatttt
      901 cgtttccaaa aagcaatatt ttttccgaaa aagaaagaaa agtttcctaa agtgttgaca
      961 tatatcggaa attgcattat actgtaagtg tcctaaagga aacgacaccg agggaggtga
     1021 aaatatgaac ccgaataaat tattagcgct tatcaagctc agaggctttt ccatgtcgga
     1081 ctttttaatg gcgattcaga tgccgccgtc tacatggtcc aagaaaatcc ggggcttatc
     1141 tgaattcact cgcgcagaaa tgtgctccat catccacgta cttaccatgg acgatgggga
     1201 agtaatggat gtttttttta atcaaaaagt ttcctaaaag aaacaaaagg agaacactat
     1261 gacgaagaga gaacttatcc gggctgaaat taatcttaaa agccgctgga gtcaagcaat
     1321 ctatcagcaa cagctcgcca aagaaaaacc ggaaagaaaa gaaatcgctg aagaagcggc
     1381 aaatgttgca ctgggctaca tgtacgctat cgcagatatc ctgcaagtca ctaaaggcgt
     1441 atcgaatgcc gacagcatcg tccgcgcatg ggccgatgaa gttgaagaaa aagaaggagc
     1501 agtcctggaa tgagaaaacc aaaactggtt atggaattag gctacgatag caccggaaaa
     1561 acattctgga caatatctag ccctaaaggc ggcgaacgac gccatggaag cgttatttct
     1621 acagactggg ggcgggtact gatgagaaaa ggtaagaaac ggggaatccc aggcgcggct
     1681 gttgcggccg tcgcgattgc cctcagcctg ggctggttca tcaaccgtcc tgccgacgtc
     1741 agtgccgcag aagtcaaggc cgacacggca gacgtccaca tcgtccagac aggggaaaac
     1801 ctctgggaca tctgccggcc gctggccgac gcgcgcggca tggacatcag agaagtcatc
     1861 tacctgatta gcgtcaacaa tggcatcaat caggatgcaa gcctgaaacc gggccagcga
     1921 atcacgctgc gattctaagg aggtgagagc gatgctcgat ttgttctgcg cggatctcat
     1981 cgaattttac agcaagcccg aaaacgtcaa ggctctggaa gaataccgga aggcccgcga
     2041 aaacaccggg aaaggaggtg ataacgatga tggaaccgga aacatgggta acgatggtgc
     2101 gcggcgagac cggcccgcct gaggg
//
LOCUS       DAWCYS010000313         2118 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2515_length_2118_cov_4.598158, whole genome shotgun sequence.
ACCESSION   DAWCYS010000313 DAWCYS010000000
VERSION     DAWCYS010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2515_length_2118_cov_4.598158"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          446 a          560 c          614 g          498 t
ORIGIN      
        1 cgatgattat gatggccggg agccattcgc acttcaagtg cagcatgacc aggcaaccca
       61 ggatggcgcc tatgaaggtc cccggcagga gccgcttcaa gaggctcatg tcgaccttgc
      121 cagcccgcca gaactgccag gcgctcatga cggcgccaaa ggtcgcggaa aatttattag
      181 tcccaatggc caggtgcggc ggcaggccgg ctgccaaaag gacgggcagc gagatgaggc
      241 cgccaccgcc ggcgatggaa tcgacgaaac cggccaggaa gccgccggcc atgatgaaga
      301 gcagcagcga cgccgacagg gtcgtatgaa aaagttggaa ttccatgaaa aaattactcc
      361 ttttggaaaa aaactcctct tgcttcgcag agcgaaaccg aggagaccct tttctcagtg
      421 ttcatatatc aagtccgccg ccgttcccgc tgtctggggc cgcagaaggc ggacgatttc
      481 cttgccgatg atccgcgcac cgtcgcggtg gcagtcgagg ctggcttcgg acatggctgc
      541 cagcttatcg ggatgtgtca acaggtccgc tacgacggaa acgaggtctt cctgcttctt
      601 gacccagcgg gcacagcctt tggcctgcat gtacgtcgca ttggcttctt cctggcccgg
      661 gatgggacta taaaggacca tcggtacctg gacggcggcc gcttcggtac aggtcagggc
      721 gcctggtttg gtgaccagca gcgatgcctg gcacatgagc tggggaatcc ggttggtata
      781 gcccaggatg ttgacgtcgt gatgcagttc gctgcgcatc ttggacagtt cgtcatagag
      841 atcggcattg tagccggtga cgacagtgaa gctgtcgacg gcgtcgaggc ggtcgaggag
      901 cgtcagggac tgtttgatgg aacccatgcc caggccgccg cccatgatga ggacgttctt
      961 atccgcctta tgggcggcat cccaatgcca gcgttcctgt tcaaaggcct tgcgcacggg
     1021 gatcccggag acgacgatct tgccggcatc gatgccctgt tcgacgagca ggtccttgag
     1081 ctgggacgcg gcgacgcagt agacatcgat ctgaggatag acccagagct gatggatggc
     1141 aaaatcggtg atgacgccga ccagggggat atcgaggcgg tgctggcgct tgaggagcgc
     1201 tgccgcccct gccgggaagg gatgggtaaa gacgaggaca tcgggcttct tttcggccaa
     1261 taccctgagc atccgccgct tgaggcccca ggcaaagagc gtcttgacga cgagtccctt
     1321 cttatatcct tgtgacgaat agtacatcaa atcgtacagc atgggaaaga catcgaggat
     1381 cttgatgtac gtttccttga cgatgttgtc gatagacagg acgtcgttgg acaggaaatc
     1441 caggtgttcc acttcacatt cttcgggaat ggtcttgacg tattcttcta tcgcccgtgc
     1501 tgcctgtgta tgccccgtac cgatggatgc ggacaggatc aggactttct tctttgtcat
     1561 gcatgatacc ctcttttata aacagcatta gtctagtagc tcatactact atggtacgac
     1621 aatttgcaag aaaagaaaag ggccgttttt catgcggccc ttcggaatcg taccttattt
     1681 gttgttgacc aaatctttga gctgtttgcc agctttgaaa gccggggtct tggatgcttc
     1741 gatctgaatc ggttcgttgt tgcgcgggtt gcggccttca cgggctttac gctgacggac
     1801 ttcaaaagtg ccaaagccga tgatctgtac tttatcaccc tgggtgaggc tgtcgctgat
     1861 agcttcgaat acagctttta cagctttttc agcgtctttc ttggtcaaat cggatttctg
     1921 tgcgactgca gcgattaatt ctgttttgtt catgatacta ggtcctcctt taaatcgtta
     1981 catctgcgta acgctaactg tctgtatttt ccttctgccg ttctatctgt ttggcctcct
     2041 gccagaacga gtcgagctca tccagcgaaa aagcatccca cggccggccc gactggcgga
     2101 cccggtcctc tacgtgag
//
LOCUS       DAWCYS010000314         2117 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4647_length_2117_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000314 DAWCYS010000000
VERSION     DAWCYS010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4647_length_2117_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          401 a          627 c          639 g          450 t
ORIGIN      
        1 gtatcgttcg agctctggga atgattgacg tggtcattgg gatggccgtt caagtccttg
       61 ccctgggcct gtaaaatctg cttggcccgg ttggcgacga cttcgttgac attcatgttg
      121 aactgcgtgc cgctgccggt catccagacg gccagaggga aattgccgtc gagcttgccg
      181 gccaggatgt catcgcaggc ctggacgatg gcgtcggccc ggtcgccgtc gacgacgccc
      241 aattccttgt tggtcaaggc agcagccttc ttcaaatagg caaagaccgt gaccatttca
      301 ggcggaattt tttccgtgcc gatcttaaaa ttctcgaaac tgcgctgtgt ctgggcaccc
      361 cagagtttat cagccgggac tttgatttct cccatcgaat ctctttctat acggtattcc
      421 atgaaaacct ccaatcagat tatataaaac atagcggact caggcgggcg ccctttgggt
      481 cgcccctacg ttgggtcccc acaggggacc attcgaggaa cgatgaacga cctacgacaa
      541 gcccatggga ttgggctgga agatgcggcc gtccatgagt tgcaggtcat cggcgatgag
      601 cgggcggaat tccatctggc tcaggatgtc ccgttcaagg tcgacgcccg gggcgatttc
      661 tgtcagcatg agtccctgcg gcgtcaggcg gaagacggcc cgttctgtaa cgaccaggac
      721 gtgctggccg tgggctgcgg cgtagtcccc ggaaaaggtg acctgttcga tatccgtctt
      781 gaatttctgg tacttccctt cccggtcgat gaccagcctc ccgccttcac agtgttctcg
      841 cagaccgccg gctgtaaagg tccccatgag gatgagtttc ttcgtgtgct gggccaagtc
      901 gatgaagccc ccgggcccgg tgacgcggcc gtggaatttt gagacattga cattgcccat
      961 ggcgtcgatt tccgccaggc ccagggcaca gaggtcgagg ccgccgccgt ggcaaatatc
     1021 gaacatgtcg gccatcttca gttccgcttc ggcattgaca gcggccccca tatcgaggcc
     1081 cgacaagggg atgccgccga tgatgcccga atcggccgcc agagtcaggc tcggcccgaa
     1141 gccctcttca gcggcgacag agccgatgat ttccgggatg ccgatgccca ggttcatgac
     1201 gtcgcctttt ttcagttcca tcgccgcccg gcgggcacag attttgcgat tgtccagagg
     1261 atgcggcggc agggacttga tggacttgcg cccttcgccg ctcagttccg gccggtagcc
     1321 gtgggtcgca aagctctgga cgtggtcctt cggccgcggc acgacgacgt aagcgacgag
     1381 gaaatggtgg atcttcacga gccgcgaatc gaggctgccc cggagcacga cgtcttcgac
     1441 ctggacgacg acgatgccgc cggaattatg cgtcgcttcg gccacttcca gctgttcacc
     1501 gatcatggct tcgcgctgga gggaaatgtt gccgtcctca tcggccaggg agccgcggat
     1561 gaagcagacc tggatgggaa aggttggata atataagtag tcgtcgccgt cgaacgagac
     1621 caggcggacg atatccttgc cttcgcggac ggtcttttcg ttggccttgc tgccgtccag
     1681 gcgcggatca gccaacgtgt gcaggccgat atcggtccag acgcccttgt gaccggcggc
     1741 gatggcccga tagagagcca gtatcgtccc ggccggaacg acataggcca ggcactgatt
     1801 ggctgccact ttatcggcca acttcttttc catgccgaaa tggctggtaa tgatcgtccc
     1861 gatgaggccg tcagctgcca ggcggtcgcc gccgcgctgg ccaccgtcgc cggtcccggc
     1921 aatcttgacc aaggtcacat tacggggatg gccttcctca tcataacggt cgtgaagggc
     1981 cttgaacagc ccgtccggcg tcccgaagcc ggcatgaccg ctgacagcca gggtcatatc
     2041 atcgccgacg agacgcgccg cttccacagg cgtaataaac tgtgtcataa cgaccttcct
     2101 ttcgtgtggt agtcaaa
//
LOCUS       DAWCYS010000315         2104 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2537_length_2104_cov_6.584187, whole genome shotgun sequence.
ACCESSION   DAWCYS010000315 DAWCYS010000000
VERSION     DAWCYS010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_2104_cov_6.584187"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          473 a          555 c          553 g          523 t
ORIGIN      
        1 tcaattttca tggtatcact caaaactttt aaataaaaaa agattttaat attattatat
       61 ttattattgt tgttgttagc taagtatagc atgagcctaa aaaggaataa agctaaatac
      121 cctttttctt agtaaagtga gttgaaattg tgacaaagtg actgtcctgc atagggtttt
      181 cccggcagat gtgctatact aatagagcta atgatttata tgcagaaagg ttggtatcat
      241 attgtgaatg aacgtaagac atctgtcttt tctaacggcc tgttatggtt cggcgccggc
      301 atttccatcg ctgaaatcct gacgggcatg cttctggcac cgctgggctt tgccaaggcc
      361 tttgcggcca tcatcctggg ccacctcatc ggcgggacag tcatgtatct ggccggcctt
      421 atcggcgggc ggaccggcgg cagcgccatg gaaacggtgc gcatttcttt cggcactcag
      481 ggtgccaagc tctttgccgg gctgaatgtc atccagctcg tcggctggac ggccgtcatg
      541 gtcgccagtg ccgccttggc agccaatacc atcgccgcca ttggcgtgtc ctggtggagc
      601 ctcatcatcg gcgccttcat tgccttgtgg atcgtcatgg gcctgaagaa cctgagcaag
      661 gtcaacgtcg tcgtcatggc ggcgctgttc atcttgacca tcgtcctcag cctggtcgtc
      721 ttcaaaggcg gtacgcccca ggtaccggct ggcgacctga cctttggcgg cgctgtcgaa
      781 ctggccgtcg ccatgccgct gtcgtggctg ccgatcattt cggactacac gcggacggct
      841 gaaaagccgg gcctggctac ggcggccagc tgtagtgcct atttcatcgc cagctcgtgg
      901 atgttcctca tcggcatggg cgccgccctg ttcaacgggc aggacgatat tgcggccatc
      961 atggtccagg ccggcatggg cgtggccggg atcctcgtcg tcctcttgtc gacagtgacg
     1021 acgaccttcc tcgatgcctt ttcagccggc gtcagctgcc agagcctggt tcccaaggtt
     1081 tcggataagg tcctggctct catcgccacc gtcctgggcg tcatcctggc catcttcgcc
     1141 gcgccggacc agttccagga cttcctctac ctcatcggtt cggtctttgc gccgatgacg
     1201 gccatcctct tgtcggattt cttcatcctc aagcaggacc acacggcaga cagcgtcaac
     1261 tggctcaacc tggtcctctg ggtcctgggc gtcatcttgt accgctttct gctcaacgtc
     1321 gatacgccag tgggcattac ggtccctgtc gtcgtcatcc tcatggtcat cagtgctttg
     1381 gtccataagg tattgaataa agcataaaga gattctaata ttgaattgat gaaatgcaaa
     1441 aatgattcgt aattagcatt gacaaaaagg catttaaaag cgtaaactta tgctcaagca
     1501 agataataat gattgaagtc ttaagaggag gcttttcaga tgaataaaca ctttactttc
     1561 gatgatatga tcaataccat gatgcccctg cccatcagcg gcaacgccaa cggcgaaggc
     1621 atctactgcc gcggtcagga actcgtcgct tccatggacc agccgaaaga aacggcccat
     1681 caggaaaaag aaaaccgcgg attcgacctc cgcggcatct tctgccagta ttgcatcgat
     1741 taataaaaag ctagtggtta gtggctagtg gctagttgtt agcggatggt tttaaattta
     1801 aggggatttg ctgcaaacta aaaactataa acttaaaact caaaaagagg ctgtcttcat
     1861 gcgacagcct cttttacgtt gttagcagct agcggttagg ggctagcgga tggaaaggat
     1921 ttagcctctc ctgctaggag aggtggcccg aatgggccgg agaggtttaa cctcgtaggg
     1981 gctcgcacgt gcggggatat gcaggaatcc cgtgacccat ttatctgaag cgggcgcccc
     2041 acgtggtcgc ccctacgagc aacgaaccac gaaccacgag ccccacgagc cacgagccac
     2101 gagc
//
LOCUS       DAWCYS010000316         2094 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2551_length_2094_cov_3.964198, whole genome shotgun sequence.
ACCESSION   DAWCYS010000316 DAWCYS010000000
VERSION     DAWCYS010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_2094_cov_3.964198"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1452..1461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          550 a          667 c          463 g          404 t
ORIGIN      
        1 ctcctatgat catgaaataa atttaaaata aatcattaca caatcaaaat aaaaaaaatc
       61 tttcatctcg aaaagagacg aaagattata cttacgcgtt accactctaa ttcctatccg
      121 aagataggct ctcaacaggt acggacatac gtcgataccc tcctgtctgt taacggccag
      181 gccccggctc cacctactcc tttcgtttca gcgagctgct tagagatgag ttctgttccg
      241 tgccgctgct gcctcacacc atccggcagc tctctggaaa cattccggaa cttactagtt
      301 ctcttcaacg cattttatat tttggattgt ttgtctgttg acgttttgta tgttatcata
      361 cccttttgta atttgcaagg ctttttccag aacttttcca aatattttta gcttaaacgt
      421 gatactttat ttaataaaat tccgtgaaga cgggtttaac gtttatcttt acatgtaacg
      481 attctttgca ggatgcgtcg cctcccccct atctttttcc ccttattaat gctataataa
      541 ataagttaca caacaaggag gaaggacatg ttagaaaaaa tccaaaattt gaatattagc
      601 gaagtcatta tccgtgccct gaatgaaatg ggctttgaag aaccgacacc gatccaggct
      661 gaatcgattc ccatcgccat gagcggcagc gacatgatcg gccaggccca gaccggtacg
      721 ggtaagaccg cagcctacgg catcccggtc ctggaaaaaa tcctgaaagc cgaaccgtcc
      781 aaggatatcc agaccgtcgt cctgtcgccg acgcgtgaac tggccatgca ggttgccgaa
      841 gaactgaacc acctggccca gttcaccaac atccaggccc tgccgatcta cggcggccag
      901 gatatggaac gccagctgcg ccgcctgcgc aaatgcccgc aaatcatcgt cgccacgccg
      961 ggccgtctca tcgaccacat gaaacgcggc accatcgatt tgagccacat cacgaccatc
     1021 gtcctcgacg aagccgacga aatgctggac atgggcttca tcgatgacat caacctcatc
     1081 atggccgcta cgccgaatac gcgccagacc ctgctcttct cggctaccat gcccaagccg
     1141 atccagcagc tggcagaaac gttcctgcac gacccgctca tcatccgcat gaaggccaaa
     1201 gaagtcacga tggacctcat cgaacagtcc tacatcgaag tgccggaccg ccagaaattc
     1261 gacatcctct gccgtctcct ggacctgcag gaaccggacc tggccatcat cttcgtccgc
     1321 accaaacgcc gcgtcgacga agtgtcggaa gccctcaaca ggcgcggtaa ctccgccgac
     1381 ggcatccacc gcgaccggac ccaggaccag cgctagacgc ggataacaca gttacgagaa
     1441 cagaccatcg annnnnnnnn ncctgaccca gtccaatcgc gatacggtca tccgccattt
     1501 ccgtgaaaag accatcgaca tcctcgtcgc taccgacgtc gccgcccgcg gcctcgatat
     1561 cagcggcgtt acccacgtct tcaactacga cctgccccag gacccggaaa gctacgtcca
     1621 ccgcgtcggc cgtaccggcc gtgccggcca gagcggtgaa gcgacgacct tcgtcatccc
     1681 ccgcgaaatg gaacacctgc ggaccatcga acgcctcatc aagcgccgca tcacccgccg
     1741 caaggccccg accttctcgg aagccctgga aggcgcccag caggccgcca tccgcagcct
     1801 gatcacgacg acagaagaag gcaacttcgg gacgtataaa gaaaatgccg aagaactcct
     1861 gtccaactac gactcggccg ctctggtagc cgcagccatc aagctcttga ccaaggaacc
     1921 ggacacgaca ccggtcaaga ttaccgaaga agcgccgctc cgcagccgca acaaccgcgg
     1981 cgaccgcaac aaccgcggcg accgcaataa ccgccgctac aaccgcaacg accgccgtgg
     2041 agaccgccgc aatcgcaaca acaaccagcg ccgtgaaaac aaacaacata aaga
//
LOCUS       DAWCYS010000317         2086 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2568_length_2086_cov_4.763171, whole genome shotgun sequence.
ACCESSION   DAWCYS010000317 DAWCYS010000000
VERSION     DAWCYS010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_2086_cov_4.763171"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          489 a          599 c          565 g          433 t
ORIGIN      
        1 tcaatgaatg cagcgtctcg gaaatcaaga agatcgcagc cctgccgaat atcgaaatac
       61 gcggcatgtt cacccatttt gccgtttccg acatgaaaga caagacgttt acgcgcgaac
      121 agtacggcca tttccgctgg atgtgcaagc gcatggaaga agaaggcatc cacatcgccc
      181 tgcgccattg ctgcaacagt gcggccgtcc tggaactgcc cgaatactac tgcgacatgg
      241 tccggccggg catcatcatg tacggctgtg aaccgtcgcc ggaaattgac atcaagcctt
      301 atgacctgcg gcccgtcatg tccctgcgct gctgcatcgc ccatgtcaag ctcatcgacg
      361 ccggcgcgac cgtcagctat ggccgccatt ataaaactcc gtcgcgccgg aaaatcgcga
      421 ccctgcccgt cggctatgcc gatggttatt cgcgcatcct gtcggggaaa gtcgacgtcc
      481 tttaccatgg ccaccgcgtc ccccaggtcg gtgccatctg catggaccag tgcatgatcg
      541 acgtcagcgg cgaagccaac gtccacgccg gtgacgaagt cgtcctcttc ggccgccagg
      601 gcgacagctt catccccatc gaagaaatcg ccgcagtctg tggcaccatc aactacgaaa
      661 tcatgtgcaa catctcgcgc cgtgtccccc gcgtctacat gaagaacggc aaagtcgtcg
      721 gccgcgaaga atacctcttc gataaaccgt agggataggc agttcgcagg ccgcagttcg
      781 taggggacgc ccaaagggcg gcccgccaga gtcatctgtc aggctgaaac ggatcgcggt
      841 agaggggcga aaaaacgtta tcattatctg taggggctcg cacgtggcga gcccacaggt
      901 atatgtagga tacttgtaaa acagattttg cgtgaacggg cgccccacgt gggcgcccct
      961 acaagaaaaa agggcttgtc gctgtgcgac aagccctttt ttcctcttcc atgattttgt
     1021 tgcaagtggt atactgtata taagatatac gcatatataa atgcaaaata atcataaaaa
     1081 ggcggtgtgt tattatgaat tggaatgata aaacggcggc caacgttaag gccatcaaac
     1141 cctcgggtat ccgcaagttc ttcgatattg cagcccaggt caaaggggtc ttgtccctga
     1201 gtatcggaga accggacttt gccgcaccgg acaaggtact ggcagctatg aaagccagcc
     1261 ttgatgccaa ggaaacgagt tataccggga attcaggcat gatggagctc cgtgaagcca
     1321 ttagccatca ttttgaacaa cgctatggtg tccattatga ccctaaagat gaaatcctgg
     1381 tcactgtcgg cgtttccgaa ggcctggcca tggccctcct ggccattacc gaaccgggcg
     1441 atgaagtcat catccccgac ccggcttacg ttgcctatcc ggcgtctgtc gaactggctg
     1501 gcgggacgcc ggtcttcgtg ccgacctatc cggaacacga tttcaaactg actgtcgaag
     1561 ctttggaaaa ggtcatcacg ccgaagacga aagccatcgt catcggctat ccgaacaacc
     1621 cgacggggac cgtcatgtcg gcagaagaat tgatgcctat tgccaaattt gccgaagaac
     1681 acgatctcat cggcgtgtcc gatgaaatct actgtgaact catgtacggc gctgccaagt
     1741 tcacgtcctt cgccaaattg ccgggcatgc gtgaacggac ggtcatcttc aacggctttt
     1801 ccaaggccta tgccatgacg ggtatgcgcc tgggctacgt ctgtgcaccg cgggaaggcc
     1861 tggcaccgat tttgaagctc catcagtacg ccatcatgtg tgccaatacg acggctcagg
     1921 tcggcggcat tacggccctc aaggaatgtg acgacgacgt cgaagccatg cgtcaggaat
     1981 acgaccgccg ccgcaaggtc atcgacaagg gtctgtgcga catggggctg cccgtctttg
     2041 cgccgacagg cgccttcgac atcttcccgg ccatgcggtg cccggg
//
LOCUS       DAWCYS010000318         2077 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_10648_length_2077_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000318 DAWCYS010000000
VERSION     DAWCYS010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10648_length_2077_cov_8.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          532 a          565 c          532 g          448 t
ORIGIN      
        1 atgtacgatt gccagctcag tttgcccggg gaatcatcca aatgcctaac ggagcacctg
       61 caaaaaactg gtttacggat acgctaagtt cagcctttca atttcagtcc gtcatggaag
      121 gcctggccta cgacgggacc gatcaggcga tagctcatgg tagcgatgtc gaagatgatg
      181 ggctgcagtt tgtcgtagtc gattttgccg tctgtcagga ccgattcgtc ggcctgctga
      241 gctacgattt tgcctacaag gatacccgta ttgtcatcat agctggtaca ttcacattcc
      301 aaggtgactg ggaattcttc gataatcggg gcattgacat gatgactctt gtggacatgg
      361 caggcggctt tttcgatttt attcacgttg tttcccgttt ctacgccaaa gtaatcggct
      421 atttcaaccg tatccttggt ggcaaaggcg acggtaaagg ccttggtatg tttgatgtta
      481 tccgtcgtct tgtgcttagc caaaaagagc gtgatttcgc cgtagtccga ttgggctccc
      541 caagcggcat tcatggcatt gggcacgccc ttttcgtcgt acgtcccgat gataaatacc
      601 ccttccggcg caatgcacgc cttatttcct tcaaattcta cactcatgga aaatccctcc
      661 ttatcctatt ctttcctaat tctatatttt tattatactc cgaatccgta cagccaggca
      721 atcaggacca gtaccagcag ataggccagg ttgagcaggc taaatagttt cagatacctt
      781 ccctgctgcc ggggacaggc ccggacgtac atgccatagg aaatgaggct tgccatggag
      841 gcgatcagtg tccccaggcc acctatatcg gttcccagca gcaaggccgc tgcctcatcg
      901 gtaaagcccg acaggaggac ggctgccggc acattactga tgacctggct tgccaaaagg
      961 gccatgaaga attcatgacc gtccagatac cctgtcagca ggtccctgaa tccaggcatc
     1021 tggctcatat tgcctacgag gataaaaaag gccacaaagg ttcccagaag ccggtaatcg
     1081 gcataacgga aataccgtgg ccgcgtcaat gccgtgacga ccacgacaaa ggccagggcc
     1141 aggcgatagt cgagcacatg cgccacggcg agcagggaaa ggaaaaacat cccgcccaaa
     1201 atccagagct gccgcgacgg agccaataca ggcagatttc gtgtccgccg tgccgctagc
     1261 cgcacggaaa tgggaatcgg cgtccccggc aagcggaaca gggacagcgt aagcagcagg
     1321 aaagacacca gggtatatgg caggagcagc gtcagaaacg atggcaaatc catagcgtaa
     1381 taaaaataaa gatacaagtt ctgcggattg ccgacgggcg tacacatact gcccagattc
     1441 gccgccagcg tctggaggac gatgacaaga gccatctgtt cctcactggc caccttgggc
     1501 agggccatca gggaaaaagg cacaaaggtc aggagggcca catcattggt cacccacatg
     1561 ctgacaaaaa aacaggcaaa taccaggacc gccgccagct ggcgcatcgt ccccaggcgg
     1621 agcaggagac gggccagcac ggcagaaaag acgccgcttt cccgcacgcc gccgatgacg
     1681 accatgagac agaacaaaat cgccagtaca ggcatattga tatacccggc ataagcagcc
     1741 gaaggcggta cggccgtcat cgacgccagc gccgctgcca ctgccagaaa aagtaccata
     1801 ttctctttga ttattgaacc aatagatttc atgctatgtc cttcattcga gttacttccc
     1861 gttctcactg cgcacctggg ccgacacgag gagcgtaaag acgccggtgc cgccgatgac
     1921 tcgcaccttc tgtttttttg actgctggca ggaaaaatca cagtcatggt tacaacgttt
     1981 aatcatggac ttcatccttt ctgatacttt tctttcatta taccatgttt cctgccgtcg
     2041 cagcaggcgg aaagcaaaaa gaggctgtcg cacgaag
//
LOCUS       DAWCYS010000319         2056 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2609_length_2056_cov_4.837581, whole genome shotgun sequence.
ACCESSION   DAWCYS010000319 DAWCYS010000000
VERSION     DAWCYS010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_2056_cov_4.837581"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          396 a          581 c          649 g          430 t
ORIGIN      
        1 ctgataatta agcggttctt cataacggga aaaggcagcg ccaggatcag tcgtttccgt
       61 ctgcctcccc ttctgcagga gcggcgtccg gtgcgcggcc aggaccagcg tccccgcttc
      121 ccgcccgggc acatagacga agagttcccg gtgcgacaag tcgctggcga actgcaagag
      181 ttcttcactg ttctgtaaaa tatggatctg cacgggcgac aaggtgctcc ttgtccggca
      241 gaccgcttcg agtgtagcca tagaagattc ccccatctct tctcaggatt agtccgatgt
      301 aatcggcagg gccggcttgg cgttgagggt caagtcggca aagcggccgt ccagggccat
      361 ataatatgcc cggcaaccga tcatggcgcc gttgtcggtg cagagtatcg gttccggccg
      421 gtagaaggca tagcccttgt cggcacaggc cgcactcatg gccgcagcca ggccgctgtt
      481 ggccgagacg ccgccggcga cggcgatttt cctggctccg cagtaggccg cggcatccat
      541 ggtcttttcg acgagcgttt cgacgacggt cttctggaag ctggccgcca cgtcggcggc
      601 attataggac tggccccgct gttcctgtgt gtgcaggtag ttgaggactg ccgatttgag
      661 gccgctgaag ctgaactcga aattgtgctt ttcatggagg gccttgggaa aggcgatggc
      721 gtcgggatcg ccttctttgg ccagtttgtc gatctgcgga ccgcccggat agggatagcc
      781 catgacgcgg gcgatcttgt cgaaagcttc gccggcggca tcgtcgcggg tctggcccat
      841 gagtctgaac gaattatagc cattgatctg gaccagctgg gtatggccgc cggatacgac
      901 gagggccagg aacggcggtt ccaaatcggg atggctgagg aagttggcaa agatatggcc
      961 ttccatgtgg ttgacgccga ccaggggctt atcggcagca aagctgatag ccttggccgc
     1021 tgcgacgccg accagcaggg ccccgaccag gccgggaccg taagtgacgc cgatatggtc
     1081 gatatctgcc aaggtgacgc cagccgtatc aagggcttcc ttgatgatgg gcatgacgta
     1141 ttcgatgtgc tggcgcgacg cgatttccgg tacgacgccg ccgaatttcc ggtggatcgg
     1201 cacctgtgtc gatatgatgt tggagcagat gtggcggcca tctttgagga cagcagccga
     1261 cgtttcgtca caactcgttt ctaaagctaa ggtatacata gatttcatcc ttttttctat
     1321 agtgaatggc cgagccattg atacttctct ttttgacaat gcatgatgca cgcatcttcc
     1381 acaggctggg aataatagtc cttgcggaca gcaagggctt catagcccag cttgcggtac
     1441 agtccctggg ccggcgcgtt ggattggcgg acttccagga agaaactcgt gcagccttcc
     1501 cggaagaggg cttccatggc cgttcgcagg agaatctcgc catagcccag gccccgtgct
     1561 gacggatgga tggcgatgtt ggccagctgg ccttcatcgg cgatgagcca gacgtcggca
     1621 taaccggcga cgctcccgtc ttccttttcc aggacataat aacgggctac gggattatcc
     1681 agttccgctg ccagggactg atgggaccag ggcacggaaa aggacgcctc ttcgatggcg
     1741 atgatatccg gcaggtcctc ccgacaggcc tgacggaccg ttactgacat ggggcaccgc
     1801 cgtggcgttt ttcccagagc acttcagctt cgctgcggcg cagggacgac ggcgtcaggg
     1861 tcatgcagtc atcgtagtca ccggcggcaa aacggacatt ggctgccagg gcgacgctgc
     1921 cggcacgggg aatgaccatc gtcggcggcg ccatgcggaa caaggggctc cggccttcga
     1981 tatcccggcg ccctttgagg gagccgtcac cgcagaagac gacgggctgg ccttcccttt
     2041 ccagggtatc caggac
//
LOCUS       DAWCYS010000320         2056 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2608_length_2056_cov_5.334333, whole genome shotgun sequence.
ACCESSION   DAWCYS010000320 DAWCYS010000000
VERSION     DAWCYS010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_2056_cov_5.334333"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          509 a          537 c          547 g          463 t
ORIGIN      
        1 actgacggta ttgcagaccg taaagccgat ggaacgaacg aagatgacgc cggacaggcg
       61 ggcgaaggca ctccagtcgt tggtccggac ttccccggga tgggctttct tttcggccgt
      121 cgccgcttcg ctgaccagag ataagatgtg cggcatctga tggtagagga cgaaggcgat
      181 gagggcgttg acgatgccga agacgaccag accgtggatg tcgaaggcat aggcacaggc
      241 tccggcaacc atggggccga tggcgaagcc ggcattgccg ccgacggaaa aggtccccat
      301 agcctgtccc ttgcggtcgc cggcgatgtt attgaccatg cgggccgctt cgggatgata
      361 gatggaagag ccgaagccgc tgaacatgga gcagaggaag atgagccagt agttctgcgt
      421 aaaggccagc atcgtcaggc agaggccgca gaggatgggg ccgagcggag cgaaccaggg
      481 cttggacacc ttgtcggaat aataaccgaa aataggctgg gaaaccgatg ataagagcat
      541 gttggcaaag atgagcgtcg ctgcgtcctg atagttcagg ttgcacgacg agatgaaata
      601 gggcaggagg gccggaatgg cgccttgcgc ccagtctacg gaaaaatggc ccgtagcgaa
      661 taggtataca taaggactca gtttcttcat gcaaattctc ctttcaggaa gttgaaatca
      721 tatctattat agcataggct gttttccttt tcatattggt gttataataa taacaaacta
      781 atcaatatat attaaaagga gttgttatta tggcatcaaa agaaatgtac gccttgggct
      841 cccaggcttc ggtcatccgt gacctcttcg cttatggaca ggaacaagcg gctatcgtcg
      901 gtaaggaaaa tgtcttcgat ttcagcatcg gcaatccgac cgtcccggcc ccggcctgtg
      961 tcaaagaagc cattgaagac atcttggaaa cgcgggaatc cgtggccatc cacggctata
     1021 cggcagcttc cggtgatgcg tctgtccgcc agggcctggc cgattacatg aaccagactt
     1081 atgacgccca ggtacgggcc gataatttct acatgacctg cggggctgca gcttcgctga
     1141 ccatcagcct caaagccctc gtcgaaggcc cggatgacaa aatcatcctc attgcgccgt
     1201 tcttcccgga atatacggtc tttgtccaca atgccaatgc caaagccgtc gtcctgccgc
     1261 cggatacgga acacttccag atttctctgg acgccctgga aaaggccatt acaccgcata
     1321 cacgggcaat catcatcaat tcgccgaata acccgtctgg cgtcgtctac tcggctgata
     1381 cctatgaaaa attggcggcc ctgctgacgc ggaagtccca ggaaatcggc catcctatct
     1441 atatcatttc agatgaaccg taccgggaaa tcatttacga tggccttccc atcctctatg
     1501 tcccgaaatt ctacaaagac acgatcatct gctattcgta cagtaaatcc ctgagcctgc
     1561 cgggcgaacg tatcggctat gtcctggttc cggactgcgc tgccgatgcc caggaagtct
     1621 atacagctgt ctgcggtgcc gcccgttcca tgggctttgt ctgcgcacct catcttttcc
     1681 aggacgtcgt cctcaaatgc ctgggccaga ctggcgacat gaacatttac gacgaaaacc
     1741 gcaagctctt atataatggt ttaagagaaa tgggctacga ctgcgtctat ccgagcggcg
     1801 ctttctacct cttcgtcaaa tcgcctgaag ccgatgccaa ggctttctcc gacaaggcca
     1861 agaagctgaa tctgctcatc gtaccggccg acagctttgg ctgccctggc tatgtccgcg
     1921 tatcgtactg cgtcgatccg gatatgatta aacggtcctt tgctgccttt aagaaactga
     1981 tggaacagtt taagtaaaaa gatgtaagaa attggataaa tttccttgac acgtcctgta
     2041 tctgtggtat actaaa
//
LOCUS       DAWCYS010000321         2052 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2613_length_2052_cov_5.573861, whole genome shotgun sequence.
ACCESSION   DAWCYS010000321 DAWCYS010000000
VERSION     DAWCYS010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_2052_cov_5.573861"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          406 a          574 c          482 g          590 t
ORIGIN      
        1 catgaccggc cgtccggatg cgatgatgtg cgctattttc tcatcattgg cggccgcttc
       61 ctgggcttcg gcttcatgcc ggtcagcctt ttcttctgcc gatggcgtaa tcatggcctg
      121 cgtctgttct ttgacagggg ttattttaag ggcatcgctc atatcgcccg acgtatcaaa
      181 ggtatagtct gccatatttc ctcctaataa atataagacc cgccaccttc ggactggtct
      241 tcatcttccc cattcgcata agctatatta ttttccacgt gttccgtaac gctttcatcg
      301 acggcatcag ccgcttcgct gacagcttcg cctacttcac tggctgtttc tgtcaaagtg
      361 tcaacatagc tattgtcatc atcactgcta tcactactat ctccatcatc actggccgct
      421 tcagccgatt cgactacatc ggccaaggtc cgttcgccgt cgaggatttg ttgtacgtcc
      481 cagcggtcaa tggtatacga attatggttc cagtctgtaa cgattgcata tccatcgtcg
      541 cctaccgtaa tacgaaggat acctgctgcc cgcatttgtg cctgtgaata gccgccattc
      601 gtcggatctg ccgttatagc attgacgcca aactgaatca aatcattcat agacggttca
      661 ttcccattgt gttcggcacg gtattgaacg gctgccccgc gaatcagctg ctgaataaca
      721 ggccaatttc ctttaaattc cgccggattg ataccgctgg catcagcaat ctgctgaggc
      781 gtgatattgt acatcggctt atattcacca gttccattct gataatcact ggccgctttc
      841 tgcaaggtat tgaggtttgc cgctgataag tgaatcccat gattattgca atagttgact
      901 atatcgttga aggactctat gccgttgctt ccgaacagtg attttaggac agtcaaattg
      961 gccccgctgc tacgagagct gccacttcca ccacgaccac cgccgcgacc gtttcctccg
     1021 gatgatccgc cagatccgcc agtacggaag ctgacatagg cactgataca gcttttcagc
     1081 gtcgtatata catcaggatt attgtagccg tatcgctctg caatggctaa ataggcttgt
     1141 gggtccagat tccctgattg atagagctgc tgcatttcca ttcgtccctg ttcgacgata
     1201 gcgtcattgg cttctttttc ctgccgtttc tgctcagcaa tgatagaagt cgccatttgt
     1261 tccgcttttt ggagttctgc ttcgtcatag atgacttccg tatgagccgt cacgccgccg
     1321 gatttttcca agccgccgtg ataaccgcct aaatggaggt gatagccact gccggcatcg
     1381 tggaagagga cctggtcaaa ggcgccgctg tcttcaaagg ttttgcgtac ttcttcggct
     1441 ttctcagcgc tggtcccttc aggaagtaca atgtcgacgg cattgccccc gttcggtcca
     1501 atgatatgct gactggtcgg tgagccattg acttcggcgt tatgttcccg tgtgcggacc
     1561 gccgaagaga tttccgcgcc gtcggcaacg cccatctggt tgagcatccc gccgatgaac
     1621 ggcagggctt gctgaaattc cggcgtcagt tctttaactt gttcgtcgat gtccgctccc
     1681 tgggtcggca ggttatatgg ctgtatctta ctgccgccgt taccggataa gtccatacca
     1741 tctaaggagt cgtaattatg cttgacaagc tgtttatact gttccgccgc ttccccgctg
     1801 ccattgtatg cccgaacgcc ttcccaatgg tcgccgccgt tttcgtcggt cttctgtttg
     1861 aggatatacg cgccagcacg gatattctgt ttcgggtccg tattccatcc aggaaactta
     1921 ctatccaaat cataggcccg cgccgtttca tcggtaatct gggcgtaccc gccgccgtct
     1981 gccatgtgca tcccttcgat ggtatcgcca ccggtttcac gcatgcctgt agataagtag
     2041 attctagggt cc
//
LOCUS       DAWCYS010000322         2050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2616_length_2050_cov_9.921303, whole genome shotgun sequence.
ACCESSION   DAWCYS010000322 DAWCYS010000000
VERSION     DAWCYS010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2616_length_2050_cov_9.921303"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          413 a          614 c          612 g          411 t
ORIGIN      
        1 gccttcgatg atgaataatt tacctttcat agaaatcctc cttcaagacg cgtatcgtcg
       61 ccaagatccc gtcagccgga ccgttcggct tatagccgcc agccatcttg cgtcggatat
      121 aggcctggca ggccgccgta atttcttcac ccaaggtcag gaccggaatg cctggcgggt
      181 agaaagtcac gttctctgcg gcaatgcggc cacaggcctt ttcaaagggc accgtctcta
      241 ccgggcagtt ccaggcctgg cgcggcgcca tgacgacgcg gggcacgggc agggcctgtt
      301 cggccaccgg gccggcctgg cagaggctgc gcccttcggc gatagtccgg caggcctgta
      361 ccagagcctg ggctgtttct tccgtatcgc ccaaggtgat cagggccagg acgtgattgc
      421 cggcgacgag ctcgacttcg atgccctgct gacgcaggag ccgttcggct gtcacgccgt
      481 cgaggccgac gccggaaaag tcgatagtaa tcttcgtttc atcgaagccg ctgacgacct
      541 gtccgtcgat gatgtcccgg ccaaagacgg tcaagccggg aatggcgttg atagcgtccc
      601 gcgccgaccg ggccaggtcc agggcccggc cgatgagttc cggcccttcc agggccagct
      661 gctggcgggc gctgtccagg gatgccagga accagtaatg aggactcgtc gactggacga
      721 cggccatgga ggcggcgacg cagtccgaca ggggaaagcc ctggcggcag tgcagcatcg
      781 atgtctgggt cagggagccg gccatcttat gggtactctg ggccacgcag tcggcaccgc
      841 aggccagggc ttcggcaggc aggtcccgat ggaagcccag atgggcccca tgggcttcat
      901 cgacgaggac atagaggcct ttttcatggg cataggccgt gagggccttc aagttaccgg
      961 cgatgccgtc gtaagtcgga taggtgaaga cgacggcctt ggcccgagga tgggcggcga
     1021 cagcccgctt cagactgtcc aggtccggtc ccaatgatac ctgacgggcc gcatcgaagc
     1081 ggctttccat atagaccgga acggcgccgc tcaggaccag gccgttgatg acgctcttgt
     1141 gggcttcgcg ggggatgatg agctcatcgc catcgccgca gacagccagg atcatggctt
     1201 cgatgcaggc cgtcgtgccg ttgatggaaa agaaagtccg cccggcgcca tagaggtcgg
     1261 cggccagttc catggctccc ttcagcgccc cttcaggctg gaacaagtca tccaaagcat
     1321 acatgacgcc caggtcccgc cgcagggcgt cgccaaagag attggcctgg taggcagaaa
     1381 tgccctgtcc cagtttatga cccggtgtat ggtaggcctt catgtgctgc tggcaatacc
     1441 cttccagtgc ttcgacgaaa ggcgcctttt gctgcttttc tctatccatg ggccacctca
     1501 tatagccgaa aggaagccgc gcagcaaggc gtggctcttt tcataagaga actgtttcat
     1561 ctgcttcagc gagcgctgca gagagccgtg caggcggtac gacggcagtc cggccggcgt
     1621 gacccagcgg taggccagat gttcttctga taatgtaacg ttatcggacg tggtcatggc
     1681 cgtaaagatg acaccattca ggaattggcc gtctttcttc tggtaactcc agatacctgc
     1741 cggagcctct aaggtgatgg aaaggcccgt ttcttcgctg acttcccggc gcaggccggc
     1801 ttcaaaatct tctgtaaagc gcaggccgcc gccagggaat tcccaatgat gcagcccttc
     1861 ggcatcgttc ttctgcaaca gcagcagctt cccttcatga aaaatcatcg ctttgacact
     1921 caaccccatc aatacatttg cttcttgcgc cataacccca atctcccatc tatacaaatt
     1981 cattgcaata aatatagtga acgtactccc gcctatagag gcgggagctt cctgcttcaa
     2041 cgagaacagc
//
LOCUS       DAWCYS010000323         2049 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2618_length_2049_cov_4.849549, whole genome shotgun sequence.
ACCESSION   DAWCYS010000323 DAWCYS010000000
VERSION     DAWCYS010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_2049_cov_4.849549"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          370 a          596 c          636 g          447 t
ORIGIN      
        1 aacggctcgg ggccaggaga tagccctgca actggggctg cagttccatc gtcggcacgg
       61 ggatgatgtg cttgccctct ttgatgcgga tggcccgggc cttggcgatt tccgacgggc
      121 acgacacatc ttcgatgcgg atgaactgcg acgcttccgg caggtgcagg gactcacaga
      181 tgcggtgtat catgtgcttc gacgtgccca ggatgagcaa tttatcggga tgtatcttct
      241 ccagcgccgc ccgcatggcc gctgcgtctt cgggcttgtt gaagatagct cggcgcacgg
      301 ctttcaggcg gttcgtctct tctttggccg accggccggc gacgatgcgg ttgtggtcga
      361 tgagcaggcc gtcgtcgatg atggcctgga tatcgtggtc ataggcgacg accagggcgt
      421 gatggctctt gcccgtgccg ctggcgccga taaaaccgaa aacctccata atctcacctg
      481 cttttcttta ggttcccttc tacatggtcg agcagagctt ttaccgcctt ggcatcgacg
      541 gtaaagacgc cgaccttttc ggggaaacgg cctgtcgtgc cgtggaaatc tgagccgccg
      601 ctgacgtacc catggcggtc ctcagccagt ttcaagaatt ctttatagcg gccgcggttc
      661 ttgggatgat agacttcgac gccgtcaaag tcatgggcca ggacctggtc cagcgtccgc
      721 ttgagcaggc ccggatgggc caggatggcc gtaccgccgg cctggcggat gagggccacg
      781 cagtcgtcga tgctgcggcg gtgataaggg acgtaagccg gaccgccgcg gtagagcagt
      841 ttatcgaaga ccgtcttgac gtcggggaaa tagcccttgg ccacgagcag gcgggcaatg
      901 tggggccggc cgacagtccc gccctggcgc agtgtcgctt cgatgccgct gcgattgatg
      961 tcatagccta agctgcggca gcggtcgacg atttccaggg ccctgttgaa gcgccgttcc
     1021 ttgaattgtg cgcagtaagc ctgcaagccg gcgtcagccg tgtcgatgcc gtagcccagg
     1081 atgtgcacat cttcgccgtc acattccgag ctgatttcaa tagccgggat gacgcggacg
     1141 cctgcggcga aacgatggct gccgtcataa gccgctaccg tatcgtggtc ggaaatagcc
     1201 atgacgctga gcccggcgtc gacggccatc tgtgttagtt gttccggtgt atatacgccg
     1261 tctgaacaag tcgtatgcat atgtaaatca acgatcatgg gacgccacgc ctccctgcaa
     1321 taagtgaaga atggatttga tgccgacgcc tgtcgccccg atgaggcccg tttcgtcggc
     1381 cttttcattg aaggcctggc cggcgatgtc caagtggatc cacggcgtgc cgtcgtggac
     1441 gaactgcttg aggaagaggc ctgccgtgat ggctcccgct tcggagccgc cgacgttctt
     1501 caaatcggcg acgtcgcttt ccaggaactt ttcatattct tcatcagccg gcagctgcca
     1561 tatcttttcg tgaacttccg tcgcagcgct gaagaaacgg ttcatccatt cctggtcgtt
     1621 gccgatcatg cccgtccgca cggtccccag ggccgtcacg caggcaccgg tcaaagtcgc
     1681 caggtcgatg agacgcgtcg cccccttttc ctgagcatac gtcagggcat cggccaggat
     1741 gagccgccct tcggcatcgg tgttcttgac ttcgaccgtc ttgccgttgt acatggtcag
     1801 gatgtcgtcg acgtgataag ccatgccgga gggaatattt tcgaccagcg ggaggacggc
     1861 caggacgttg acgggatatt tgagcagcat caaagcccgc atgacgccga cagccgctgc
     1921 ggcaccggcc atgtcgtcct tcatgaattc cagattggca tggctcttga gggacaggcc
     1981 gccgctgtca taagtgacgc ccttgccgac gatgcccagg acttcccggc ggttagggtc
     2041 gccctggta
//
LOCUS       DAWCYS010000324         2043 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2629_length_2043_cov_5.378270, whole genome shotgun sequence.
ACCESSION   DAWCYS010000324 DAWCYS010000000
VERSION     DAWCYS010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_2043_cov_5.378270"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          412 a          503 c          569 g          559 t
ORIGIN      
        1 tcgaggggaa gatttccgtg cgttgttccc attcctgcgt cttcccgacc ttcacgacgt
       61 acttgatatt gtaaatagat aaagcatccc agtagaaata cagattggcc ccgttctttt
      121 ctacccagaa gcccgtgacg ttcgggacaa aacaggacag gtaggcacgt tcaccttctc
      181 cgaattggtc gtaataggct atatacaact ctttaatatc tgtgttcgga tacaagaaga
      241 cgttatccac ggcctgatac ttcacgccgt caatgtatag gttagcgccg atacagttag
      301 acggtatctc caggaaggta ataagtgtcc cttcattgtt cttggtaaag gatatgtcag
      361 acggtgcgga cggcctccgt ttgttatacg tgatggtccg gccgttggat atcttgccgt
      421 gcttgctgat ggcaaatagg tatatcttgc cgctggccgt cgtggggata gacaggcttg
      481 atgtagctat ggtcttttcc agcaggccgt atgcgttgcc gacgtcggca ttggtgcgga
      541 cttcgtagta agccaggtcg gcgtcgtcga cggcgtccca gttgagtacc ccgcccagcc
      601 ggtcgaacgt cagcgtgaag ttacgcggca tggggaagtc acctttattc gaggcgtcta
      661 catcgtctgc cgtgaatcca ttggctacgt tgacctgctc attgactgat ttcagatact
      721 tccgcaagag ggatataagc tgtctaccgt cgccctggat agcggtcgga aggtctggga
      781 atgtcagtgc ttgtttcttg tattctgcca tagcatcaac tcattcctgt gctgattgcc
      841 tgctgtaagg cattgacgat attcgtatcc tggctaatgt cgtactcgtt ttcattgagt
      901 gccaggagga ccgccgactt gacgatgata tcgttgatgg cgtcatggtt gaacggcagc
      961 tctttcgtcg cgctttcgat aagggcaggt gtggcaaaat acctgaattt cacggcggta
     1021 atgtccgggt cggtgatatg cacggtcccg gccgtcatgg ccaggggata cgtcccacaa
     1081 gcgctcatat agtttttggg gatggaatcg ccgtccctca tggtcgtttc ttctacgagt
     1141 accggccatt tagccccaat gagcaggctt gccacctgtt gcgtggcggt attgaggaat
     1201 tgcaggcaac gttcgtcgct gtattccttg ctgatatcgt gcgtctcctg ccggatacgg
     1261 gtaatggcat cttctacttc cattcagtca ccccctagca gatgaacggc atacgctttt
     1321 ctgtgttggc gtatttccgc ataggtacga cattggccag ggccgcttcc acggcctgtt
     1381 gcatggtatc gccgtccggt gtctgtgtga ggaccataca ggccagcttg cacaaggaat
     1441 caaggaagac ggctggcagg tcgattttcc cggtatcgag gtcagtgata ctaaggaacg
     1501 cggcgttata gagcatatcc acgtccttta caccggcata aagcttgttg tggaatatct
     1561 tgtattcatc ccagcgcggc ggccggatag cgtctcctgg atgaaggtcg cgcccgtggc
     1621 cgtcaacgat acggacgaga gtaaggaagt cgtcgggcag atctacgccg gtaatcggca
     1681 tgtcgatatg ctctttcggc gtcggcttta cagccgtttc gtcggacggg tccgtcgata
     1741 gactggcgtt gtactcgtcg atttcccggt tcatgtcgtc ctgccggtag tgctggacct
     1801 tttcgaggaa gtcgctgttg atgtagtact gattgacgta acgcaatact tcgttgatgg
     1861 cctggaggat gtcatagtcg ctgtactgga cttcgttatt atcccctagt ttgtagcgga
     1921 tgagctgttt gagtgatttc gcggtaatca tcccagcaac accgccccac gccacgtacg
     1981 tttcttgtgg ttgacggcga actgcttcca tacagtgaag aactgctgga tgtagtactg
     2041 gta
//
LOCUS       DAWCYS010000325         2039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2634_length_2039_cov_4.331653, whole genome shotgun sequence.
ACCESSION   DAWCYS010000325 DAWCYS010000000
VERSION     DAWCYS010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2634_length_2039_cov_4.331653"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          351 a          613 c          593 g          482 t
ORIGIN      
        1 gggcaatacg aaaccgaatg ggcagacgca ggcccgcaag gctgagacgg ctcaggccaa
       61 tgccagccgg acgacgtctt cgtcccgcct gggcagcgtc atgcgcggta tcggcctctt
      121 tgccggcggc atgttcctgg gcagtatgct cagccatctc ttcggctggg gttccctggg
      181 ctggggtgcc gatatcctcg gcctgttagt gaatatcttc atcttctacc tggtcatcaa
      241 aggcgctgct gccctctggc gctggctgcg cgaccgccgc agaaggtgac gtatggacga
      301 atcgtttatg aaagaacggg cgattttgcc gctcatcgct tctatggccc tgccggccgt
      361 cgtgtcgatg atcgtcaatt cgctctataa tatcgtcgac agcctgttcg tcgccatgat
      421 cagtgaacag gccatgacgg ctgtgtccct ggtctttccc atgcagaact tcgtcaatgc
      481 cgtctgcatc ggctttggcg tcggcctcaa tgccattatt tccatctgcc tcggcgccgg
      541 ccagaagggg acggccgacg tggctgcgac acacggtgtc ttcctggctg tcattcatgg
      601 tctggtcatg acggcaggct gtatctgggg cgtgccgtct tttttgcggg cctttacgca
      661 ggacgcggga ctcatcgacc tgggcctgcg ctatgccgtc atcgtctttg ccttttctac
      721 gatcctcagc gtcgacctgg tctatgaaaa gatgttccag gccgtcggcc gcatgcaggt
      781 caccatggcc ggcttgtccc tgggctgcct ggtcaatatc atcggcgacc cgattctcat
      841 ctttggcctg ggaccgattc cggccatggg gattgaaggc gcggcctggg cgacgggcct
      901 gggccagacg gtgaacctcc tcttttatgc cgtggtctgc cattggcggc cgctgcgggt
      961 cgaagtttgc cgctgccatc tccactggga catcgaactc gataaacggc tctatggcat
     1021 cggcatcccg gcggccctca acatgggcct gtcgtcggtc ttcctcgtcg ccctgaacca
     1081 tctcctggcg cctttttcgc cgacgtatat tttcatcctc ggcgtctatt ataagctcca
     1141 gacctttctt tctatgccga caaatggtat cattcaaggt gtgcggccgc tgatcggcta
     1201 taattacgga gctggggaac tgggccgcgt gcgccgcatt tatcggacga cgctgttcct
     1261 caacggcctg gtcatggcgg caggcctggt catctgcctg gccgtaccgg acgtcctcat
     1321 gggctggttc acgtcccgtg aggaaaccat cgcagccggt gcgtcggccc tgggcatcat
     1381 tgcctgggga ttcgtaccgt cggccttgtc tattacggcg tctggcgccc tggaagggct
     1441 ggggaagggc aatccgtcct ttgccatcac cctgttgcgc tacgtcgtca tcagcctgcc
     1501 ggcggcgtgg ctcctctgcc gcgtttatgg accggacggc gtgtggcacg ccttctggat
     1561 ctttgaatgt ctggcagcac tggcagcctg gaagatttac gaccactatg ccccagccgt
     1621 tacgaaagaa aaggagacga catgaagtat aagatcattg cttgtgatat ggatgaaacg
     1681 gtgctcagca gcgacgcgac gatgtcccgg cgaaatatcg aagccattac ggcagccatt
     1741 gccaaaggcg tgaaattcgt gccctgtacg ggccggggct tccgttccat cgaaggcgtt
     1801 ctgaggactc tgggcctcta cgaccaggcg gaccagtatg ttatcggctt caacggcgcc
     1861 aatattacgg aaaacaaggg cagccgctcc ctcttctggg acccgatccc ctttgacctg
     1921 gccgaccgga ttttccgccg ctgctgtacc tatggtcaat gtatgcatag ctatacgcga
     1981 gatgacgtct acatcacgca tattactcca tacgaagaag ccttcctcca cggccgcag
//
LOCUS       DAWCYS010000326         2038 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_12854_length_2038_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000326 DAWCYS010000000
VERSION     DAWCYS010000326.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12854_length_2038_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          543 c          524 g          519 t
ORIGIN      
        1 atcgacctct gttcgacatg ggccctgtat gacggcgaaa tgaagatgag ccagatgatc
       61 tcgaagatta tgaagctcgg cgcctttctg tttatcatta tgtattggaa cgacatcaac
      121 cacgctatca tggtatcctt ccaatacgcc ggcctcacag cggcgaatgt gccggtgaac
      181 ggcgatgtca tcgagcccag caagctgctg gataacggct tcaacgtctg taagacactg
      241 ttcgatggct tgtcttcggt cgggcttaat atcggtaaag ccctgatgta cgtcattact
      301 atcggcctgg tcatgttctc gttcttcatc atggcgttac agattcttat tacgaaaata
      361 gaattcaata tctttgccag tatcgccgtc atcctcatgc cgttcggggc catccggtat
      421 acgaatttcc tctgtcagcg ctgtatttca gcggtttttg ctttcggtgt gaagctcatg
      481 gtcatgttct ttctcatcgg cctcgttcaa acgattgtcg gcaaggtcga cgccatcccg
      541 aaagatgctg tcacttttgc cgtcatgctg aaatatgcca tcagctatct tatccttggc
      601 tatctggtct ggaaagtacc gaacctggcc gctatgatga tgcagggcca gccagctttg
      661 gatgctccca tgcagtccat caagaattac ggtcagcagg cggcgaagac agccctgaac
      721 gtggcgacac tgcctgttcg tgccggccag aaaggcctgc aaagtttcgg caacttccaa
      781 agtacgctga acacagcccg tatccgtgct aacggcggta cgctgtataa cggcaatcat
      841 gaaccgttcc agacggcagg ttccggacgg accgtaaaga atgaatttgc ccgtgaaata
      901 ttccggcaga agttcggcaa cttcctcagc ggtaacgtcg aaaaaggcgc tcagaacgca
      961 ctggaacact cgaagtcctg gcgtaatgtc cgttccgggg catactccaa ggaagggtat
     1021 acaccgaata tgcccgatgc ttctgaatcg ccccgctctg cttcttcaca ggctccgtca
     1081 gggaaatcca ataagaagtg aggcggtgag tgatgaaatt tgcaatccgt atcctctgcc
     1141 ttctgacgct gctcctgacg atgtgcatcc atgtcttgga agctccgcct attgatggcg
     1201 gtcctgtcac cagtccgttc ggaccacgtt ggggacggga ccataaaggg gtcgatatcg
     1261 gcgttcccga aggaacgccg gtagtagccc tttcgatggc tattgtgaag gggcccctga
     1321 cggtggtttt ggctactggg tcacagttac caataccagt accggcgaag tctggctttt
     1381 cggtgattta tctgacgcct ctgaaggctg tgcccgtggc aacgtccacg aaggagatgt
     1441 cgtcggtttt gttggtggtg cttttgatta ttatgattca actggcactc acaactactc
     1501 cactggggat catcttcatg cagaatatca tccctatggg tacgaccctt atcatgggca
     1561 gatcgacccg gtcccctatt taatcatgct tggcgtcaac ctgactggga ataccagcag
     1621 ctctggctct ggtggcggcc gtttcggtca tgataacgta ggcatcccgt ggggtatcaa
     1681 ggagatgtac gaaatcggca atgatttgag caaggacatc gaacagttct cgaaatgggc
     1741 caataaagcc tttacactgg tccataaatt cgccgtttcc ctgctcgtcg cgctgtgcat
     1801 catcgacctg gccctgccta tcctggtggc gggtatgcgc ttttccaagg aaacgttcat
     1861 ccgcaaggtc atgaagtatg ccgcactatt cggtattgtg gcctgctgga ataagttcac
     1921 cgataacatc ctgctcggca tcgtcaaatc ggctgccagt acatacagcg gcagcttcga
     1981 cctgatttcc tcgaacatgt cccagccaca gcttctccag ccgcacgctt accggagg
//
LOCUS       DAWCYS010000327         2034 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2638_length_2034_cov_6.068722, whole genome shotgun sequence.
ACCESSION   DAWCYS010000327 DAWCYS010000000
VERSION     DAWCYS010000327.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_2034_cov_6.068722"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          501 a          578 c          476 g          479 t
ORIGIN      
        1 cgacgagctc atcgtccttc aagggaatcc agtcataggc cacatccgct ccgggcttgg
       61 cggccagaca gcagtcgacg gccttctcat tgatccactg ggccaattcc tgattcgtcc
      121 cttctaacaa atggacttcc accttgggat actgggcctg gaaacggcgc aaaatcttcg
      181 gcagccacgc cgcggcggca ctataataac tgccgatatt gaggaccccg gccaggacgc
      241 cttgaatggc cgcccccaag tcggcggcct tttgcgaagc cctgacgact tcctgaaagg
      301 ccggcaccat ctgctggcca ttggccgacg gccggacgcc ctgcttgttg cgcacgagca
      361 gagggaagcc gatttccttc tccagcgaac ggatcatacg ggtcatcccc gactgagtat
      421 accccagttc cttgccggcc gccgtcagac tgccatattg aatcgccagg agcaaagcct
      481 gacattttat actatccata tcacttattc acccatttcg tcatgacata catgctaaat
      541 caccgtttgt gtcatatctt attgtaccgt ataataaggc catccgtaaa ggattagacc
      601 caaatgagga cccattatga ctactttatt acccttcgcc tcagactata tggaaggcgc
      661 atacccggaa taaccatgaa tttcacagct caaagtggat acggtactga ttattcgttc
      721 atcgaaattc ggtcctgtcg gccctaacgg ttcaaaaagg ctctgggacg gcccctatgc
      781 acgaggccgc tgtccgtgta ttttttcggt ccgcaggacc gcggcggcca gcgaactgcc
      841 tcctaataat tcactgcgtt ttgttgaaac atataacgtt taacgtcaat ttacttgaaa
      901 ttttttgcga aaatcatttg acaaagtccg tttttgatgg tagacttacg tcaacaactt
      961 gatagtgttg caatgaagag aagagtacac gataggaatt ggcacagaga gttcccattt
     1021 ggtgaaaggg aatgcaaggg tcatcgtgga agatggactc cgagcagatt ggtcgaacag
     1081 ttaggccgtc cgggcgtgcc cgttacagca ctatagtatt caaggctcat ttgcctgcgt
     1141 acctgaagag gctcatcctg tgagggatga gtaaaattgg gtggtatcac ggtgcttccg
     1201 tcccttactt gggacggaag ctttttttat tacaaggctt tcaagtttat attacattat
     1261 atatttttta atattagggg gaaatgtatt atgagcgaaa acacaaacta caaattcgaa
     1321 acattacagc agcatgcagg tcaggtaccg gatccggcaa cgggtgcccg cgccgttcct
     1381 atttaccaga ccacatctta tgtctttaaa gatacacagc aggccgaaga ccgcttcgct
     1441 ttgaaagacg ctggctacat ctacagccgt ctgaccaacc cgacgcagga cgtcctggaa
     1501 aaacgtatcg cagccctcga aggcggcaca gccggtttgg ctacagccag cggcgcttcg
     1561 gctatcgcct actccatcat caacctcgcc ggttctggtg acgaaatcat cgctgctccg
     1621 accttgtacg gcggcacagt cgaactgttc agcgaaacct tcaaacacct cggcattacg
     1681 acgcacttcg ccgatcccga cgacccgcag tcctttgccg aatacatcaa cgacaagacc
     1741 aaagccattt acctcgaaag cctcggcaac ccggccatca acattccgga cttcgaagct
     1801 atcgccaaaa tcgcacacga caacggcctt ccggtcatcg tcgacaacac ctttgctacg
     1861 ccgttcctct tccgcccgct cgaacacagt gctgacgtcg tcgtccattc ggctacgaaa
     1921 ttcatcggcg gccacggcac gaccctcggc ggcctcatcg ttgaaaacgg caaatttgac
     1981 tgggctggct cgggccgttt caaagaattc gcttcgcctg acgaatccta caat
//
LOCUS       DAWCYS010000328         2030 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2650_length_2030_cov_6.567089, whole genome shotgun sequence.
ACCESSION   DAWCYS010000328 DAWCYS010000000
VERSION     DAWCYS010000328.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_2030_cov_6.567089"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          503 a          570 c          541 g          416 t
ORIGIN      
        1 accagatact caacccgcgg gcgacgtggg acaacaagaa gtcctacgac atcatggcca
       61 agaaagtcgc cgccatgttc tacgaaaact tcaagaccaa gtaccccgac atgcccgaag
      121 aaatcatcca ggctggaccg cgggtgtaga ggggcgcccg tcccgggcag gccgcaggcc
      181 gcagttcgca agccgcaagc cgcaggccgc agcgacatca tagatgtcgc actgtagggg
      241 ccgtcctgtt aaggcggccc gttgtgggaa tcccgttatc gttcatcata tagttgcctt
      301 tcggacttgg ctcaatttga cgggaccgcc gatgaagatc tgctgcagtt ccccttgttc
      361 gccgactttg gcggcaatgg ccagggtacc gccgggctgc ttcatgtagc aggccacgtt
      421 cttatgggtc gtgcaggcca gggccgcggc gacggctgcc gatccgctgc cgcagctgtt
      481 ttcccaatat agcgtgtccg tatcgcggac gtagacagcg ggaatcatgg tctgttcctt
      541 cgtatcgcag agaatcaggc cgtaagccgg gaaatcctcg ccatcgacgt aatcagccag
      601 gatatgccag tatttctctt tatcaatgcc ttctaaatca ggcacgaagt ggacgaagtg
      661 cgtaatcccc ggcaaatcga cgcggaaaaa acgggccggc acatcgccgg cgtcgacgag
      721 gacgccgctc acatcttcgg gataaggcat ttcaatgaag gcttcataca ggccatcgcc
      781 tttctgggca accttagccg gcaggaccgt atcgcagccc gagcaggata catcgtatac
      841 gccttgtgtc ccgccgtcac aggccagggc ccaggccgcc gccgaccgcg atgcattgcc
      901 gcagaactcg ccgcccatca tgtcgacgcg caggggcttg cccgcttcgc gggtcacata
      961 gccgacctgt tccgcttcga tgcccggcag ggccaataac ttcgccgcca aagcactgtg
     1021 ttcctctttc ggcactggcg tcagcaccag aatcgtcgta ttgccgctag ggtcggctac
     1081 taaataatct atggtcatgt ccatcgctcc tttagtctag taaaatatgt tcctattata
     1141 ccatgatttg gtgaaaaaag ggcttgtcgc acgacgacaa gccctttaaa gttgttagtg
     1201 gctagcagtt agttgttagc ggatggtttt aaatttagta gaaaacgcag gccgcagttc
     1261 gcagttcgcc ggccgcaggc cgctgtaggg gcttccacgt gggaagcccg tttaagaggt
     1321 tagcggatag cgaccggcgg gacggcaaca gacgtcccta tacgcccaaa atatggtata
     1381 atgtaattga ataggagtca tgctcccatc gaatttatga taaaggggtt gatcttatgt
     1441 cgaaaacggc agcagatatc atggaaaaaa atgtcattac ggcgcagaaa gatacgtcta
     1501 tctttgacct cgtcgatatg ttcgtagaaa acaacatcac ggccattcct atcatcaaag
     1561 acgacaagga aatcatcggc atcgtcaccg acgcagacct cctgtacaag gaagtcaagc
     1621 cctgtgtccc ccaatacgtc aacctcctgg gagccaacat ctattacggc aacctcaagg
     1681 aataccagca gggcttcaag aaactctttg cctgcacggc cgaacaactc atgaccaaaa
     1741 aagtcatcat ggccggcccg gacgccacca tggaagacct ggccagcgtc atggtcgcag
     1801 aacacctgaa agccatccct atcgtcaagg acaagaagct cctgggcatg gtagaacgaa
     1861 agaacatcct caaacaactc tacaatgaat acggcgaaaa tacacagtga aaaaaaaagc
     1921 gctgtcgcat atgcggcagc gcttttttag cagttagcag ttagcggctg gcggctggca
     1981 gcccggctct cctgctagga gagctgtcag cgcagctgac tgagaggtta
//
LOCUS       DAWCYS010000329         2018 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2667_length_2018_cov_7.089659, whole genome shotgun sequence.
ACCESSION   DAWCYS010000329 DAWCYS010000000
VERSION     DAWCYS010000329.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_2018_cov_7.089659"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          511 c          545 g          533 t
ORIGIN      
        1 gtcgcatcgc gacagctcct ttcaagttgt tagtggctag ttgttagttg ttagcagatg
       61 ggtttaaatt taaagctttt ctctgcaaac tacaaactcc gcactacaaa agataaagcc
      121 aaaaaggggc cttgcatgat ggcaaaaacc ttcgtgcagc ggccccgacg agctacgagc
      181 cacgaatctg gcactgtacg aaattcccac atatacgaaa gcgggcgccc tacgtgggcg
      241 cccctacaaa ttactaaata cggatttcgt tagtatcgcg gtagatgagc cattcggcga
      301 tattgttggc gtggtcggcc atgcgttcga tatatttgat gaccaggacc aggtcgatgt
      361 aatccttgga gttttccgga tgagccgcca tttcagcagc caaggtttcc ataaggttat
      421 tgaaagcctg gtcgacgata tcgtctttat cgcgcatgag gctggcctgg ctcgatgtgc
      481 cccgttcttt gtagaagtcg atgacatcac tgaccatgga ggacatgacg ccgtacattt
      541 ccttaatgcc ctgcggcaag ggtacgttgt gatgggtctg ttccaaatgg atgacgtagt
      601 ggctgatgtc ggcgcagtga tcggcaatcc gttctaagtc ggagaggatt ttcaacgtcg
      661 ccatcaagct ccgatagtca gccgctacgg gaccctgcat catactgatg gtcaagtcct
      721 tcttcataca gttcttgacc aaatcatcga tgacgtcgtc gccatcgtaa atgcgctgag
      781 ccagttcgac atctttgttt tccagcgctt tccaggcatt gtcgacggcc tgttcgacct
      841 tcatgcccag gttgatcata tcatttttca attctgttac ggatttttcg taaatttcca
      901 gcataagtgc ctttcctcct cttaaccgaa acggccggta atgtattctt ccgtcttggg
      961 attggacgga ttggtgaaca tatccagggt gttattgtat tcaatgagtt cgcctaagag
     1021 gaaaaatgcc gtcttgtcgg aaatgcggcg ggcctgctgc atgctgtgtg tgacgatgac
     1081 gatagtatat ttttccttca attccaaggc caggtcttcg attttctgcg tcgaaatcgg
     1141 gtccagtgcc gatgtcggtt cgtccatgag gatgaccgac ggatccgctg ccagggtacg
     1201 ggcaatgcag agacgctgct gctggccgcc ggagaggccg agggcgctct tactgaggcg
     1261 gtccttcatt tcatcccagc aggcagcctg acgcaggctc ttttcgacga tggcgtcgag
     1321 ctgcttctta tccttgatgc cctggatgcg cggaccatag gcaatatttt catagatgct
     1381 cttcgggaac ggcgtcggct gctggaagac catgccgaca cggtaacgca gggccagggg
     1441 gtcgacttcc ttgaagatat ccttaccttc aaagcggata tcgccagtga cccggcagcc
     1501 ttcgacccag tcattcatgc ggttcagtgt cttgaggaac gtcgacttgc cgcagccaga
     1561 cgggccgatg agggccgtga tttcattctt cttgattttc atgttgatgt ctttcagcgc
     1621 ctggaactca ttatagaaga ggtccatgtg gctgacatcg aaggtatatt ccgctgtacc
     1681 ggcagcagcc tgcgaacttt cctgcacggc ttctgcagcc cgtttctttt caatcgtcgc
     1741 tgcggcaaag ccggtcagcg tcgtagtcat ttctgccatt atttatttcc tcccattttc
     1801 ttgtcaattc gatagctgaa atagcgtgcg gccaggctga agacgaggac catgatcatc
     1861 aggatggccg acgacgcagc agcctgctgg gcagcgtctg catgaagggc ttccgtacgc
     1921 agggcccaga tgtggagggc cagtgtttcg ccgggacgga aggggttgag cgggcacgac
     1981 gtagccgtaa tatcccagtt cgtccagtcg atgtctgt
//
LOCUS       DAWCYS010000330         2016 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2670_length_2016_cov_4.542580, whole genome shotgun sequence.
ACCESSION   DAWCYS010000330 DAWCYS010000000
VERSION     DAWCYS010000330.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2670_length_2016_cov_4.542580"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          370 a          628 c          587 g          431 t
ORIGIN      
        1 atggcagacc agtatgcccg cggctgcccc atgctcgtcc tggcctggag tcgcttgccg
       61 atggggctgg aaggggcggc tgccggtgcc gccttcggcg ccgtcccggg ggcggcggca
      121 gggctggtgc tcatcgcttt cctgtattac cgtcacagcc gccatcccat gcccgatgac
      181 gacggcgtct tcgtcatcca gtcgccgcgg cgcatcctcc gccggctcat cgtcctggcc
      241 gtgcccgtag cggcggccaa catgctcctg ccggccgtgg ccagcatcga cttgttcatc
      301 gtgcccctgc gtctggaagc agctggctac agcacccatc aggcgacggc cctttacggc
      361 tatctgacgg gcatggccaa cggcctggtc cagctgccgg ctatcttgac catcgccttg
      421 gcgacgagcc tcgtcccggc cgtatcggct gccttttccc agcggaatcg ggacgtcatc
      481 ctcgaccgga ccgacacggc catgcgcatt gccaacgtca tcaccgtgcc ggcctgcctg
      541 ggcctggccg tcctggccgt acccatttcc cggctcctct atgcgacgcc ggcagcgggg
      601 ccggccatct gcgtcctgtc catttcggtc ttcctcgtcg gtctccagca gatcaccagc
      661 ggccttttgc agggcatggg ccatacggcc gtcccgctgt acaacatggc cgccgcggcc
      721 gtcgtcaaga ttatcctcag ctggcacttg acggccattc cctggctggg cgaagtcggc
      781 gccgcctggg cgaccaatgc cgatttggcc gtcgctacgg cgctcaatct ttactttgcc
      841 cgtaaataca ttgcctaccg cgtccagtgg ggctacatcg ggaaactctt tctggccggc
      901 gccgccatgg ccggcacggc ctggctcgtc caccacgcct tgttcgtcct cttcggcaac
      961 gccgtggcca ccatcgccgc catcatggcc gcagccctgg tctacctcat cggcctcgtc
     1021 gtcttccggg ccctcacggc agaagatatc gaaaaaatcc ccgtcgtcgg gaagaaagta
     1081 agccggcgga tacatttgta ggaaagacgc aggccgcagt ccgcgggtgg caggccagaa
     1141 aaaaatacac ggcctgcggc cgtatgcgta ggggctgtcg cacgaaggct tcggccatca
     1201 tgcgaggccc cttttacttt atagtttgta gtacggagtt tgtagaagct ggtcttaaat
     1261 tttttaccat ttgctacaaa ctaaaaacta caaacttaaa actcaaaaag aggcgatgtc
     1321 gtcagtttga atctgttgcc gcgcatgtca tcggggatgt acacaggatt cacgcgggac
     1381 gccttaacag gacgtccccc tacgatgcgg tctgcgaccg gtagggacgt tagccccaaa
     1441 actcgatgaa cggctctagg ctgcggcctg cgggacggcc tttggccatc ccttgctttt
     1501 tcccgttctt tttgctatac tggaaacaaa tagttgagca aatcaactaa ttactgaaga
     1561 aaaggggatg cagcacatgg attatttgaa ggctggtcat gatttcagtg tcatcaacca
     1621 gcgttcccaa gcctatgtgg tcgaagcgtg ccgtccttgg aatttgtctt attcggaaca
     1681 tgtgacgctc ctgagtttgt acagccacga tggctgccgg cagaatgagc tctgtcaggt
     1741 catgcaggcc gacaaagccc tggtggcccg gaatctgaag acgctggagg cgaagggatt
     1801 cgtcagccgg cgccagcagg ggacggaccg gcgttacaag tatctctatt tgacgcccaa
     1861 ggcccgggag ctgcaggata cgatggaaga gattttaagg aaatgggtcg acgtcctcgt
     1921 ccagggcgtc gatgaggtga cgctggggcc ggcgttgcag ctcatgcatc cagtggccga
     1981 ctctgccgct gaagccgata ttgcgtccct taatcc
//
LOCUS       DAWCYS010000331         2004 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2683_length_2004_cov_4.831195, whole genome shotgun sequence.
ACCESSION   DAWCYS010000331 DAWCYS010000000
VERSION     DAWCYS010000331.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_2004_cov_4.831195"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          369 a          561 c          540 g          534 t
ORIGIN      
        1 ttcctgcctg atttcttcgg acatggcatc gctcttatcg ttagcaatgt cgcgaagttc
       61 ctgtgccaga gcatccatac gctgcttccg ttcgcgaagc gctgccaaat gcgtaccgcc
      121 ctggttcatg gtagacgcat ccatgagggt attcgtatca aacgattcat cggcccgctg
      181 cgcaaagacg ccggtcttga tttccaaatc catgccattc tgtaccgctg tatctacctg
      241 ttccggcgtc actatatcgc gctttacgag gtcgttcagt acgttgacgc cttccggagt
      301 ccgggccagc tcctgggcat cggtgtagat agtgcccata tcgtgctgtt cggcctggct
      361 ttggatgacg gtttgatacg tggacggaga atctgtagcc agcttattat ctgatttatt
      421 agccacaagg tctgtaatca tttgtttttc tacgttgcgc tgatattctt cgcgccagga
      481 gtcgactttg aggctggcaa tattacgcat ggcgttatag tgcccgactg tatgcatccc
      541 ggttcccata gcgcccatgc cgagggcggc cgggacagcc tctaccatcg cgtcgacggc
      601 gttgttgagc acatcgccga cggtgttcca gctgccttta tgatggattc catattctac
      661 gtttgttgcg acgtcaccga tgacggactg tacaccttct tccgtcaatt cagacagcgt
      721 cccgcgggcg tactgtttcg cccctgcgag agctgcgatt ttagccaggg ctaatttgcc
      781 ctggtttact acgtcccgtt gagcagccgc attggtcagc agggattttg cggcatcttt
      841 gccgaaggcc gctttaatcg gaccatagcc aaattccaac aatcccaatt cgacggctcc
      901 gttcagcgcg ccgacgacgg cactatcagt cagcatgttc gcccggctgt ataaaggctt
      961 tccattcgac tgctgctgtg ccatctgcca atagcggtcg gccatggatt gtttgctgat
     1021 ttcattgtac aggccggtcc gtaaccccca ggcggccccg gtcgcaccgg cggcggccaa
     1081 gatggctgcc gagccagccc cgagtgttcc cggaccagcc gcggcggcag caggagtggc
     1141 cgtggccata gccagggcca tgcccttcgg gacatactgg agagcacgca acccctgtgt
     1201 gccgtaaatg gtcagctgct gcacggtgtc atagacgatt ttccccagtg ccgatgtagg
     1261 ccggtcttct tcctggtagg ctttgaggcg ctgtgtgatt tcgtcgactt ccggtttgac
     1321 ggtatccagt tcttcaccat tgacagcctt catctgtgca tcatagagtt tcaccatatc
     1381 agagccggag ttaaaagcgt cagcaaagag ttccccggca ctgttgaagg catcgccgat
     1441 gcgttcaaag atgctgcgag tatcattcaa gtcccggtga ttccgcaagg ccagggccgc
     1501 accaacgggg ttttcctgcc ggaatttcgc caattccgga taatatttat ccagcgtttc
     1561 tggcgaatac cagttattgc caggtaggaa gtcattgata gtcaataggg tatcgcgttt
     1621 ctttgtttct tcaagcagtt cgggatgatc taccatatac tgcggcgata cgccgagcat
     1681 aggggcatag gtatcggcat ccttgagtac ctgcgcattt tcgttgaaga gattgcgcca
     1741 aatgtcgcgg gccgaatcga cgacccattc agcggccttc tgccattccg gcttttcttg
     1801 cggctctggt ggggtcaagt tccccgtgat ggttggcgtt gtcagtacat ccggtatttt
     1861 acgcatgacc ggccgtccgg atgcgatgat ttccccaata ttctcatcat tggcggcctc
     1921 ttcctgggct tcggcttcat gccgttcagc cgtgtcttct gccgatggcg taatcatggc
     1981 ctgcgtctgt tctttgacag gggt
//
LOCUS       DAWCYS010000332         2004 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2681_length_2004_cov_6.979990, whole genome shotgun sequence.
ACCESSION   DAWCYS010000332 DAWCYS010000000
VERSION     DAWCYS010000332.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_2004_cov_6.979990"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          402 a          553 c          548 g          501 t
ORIGIN      
        1 tcgtgaggac cagggacggc acgggggcct ggatgggctc gccgatctgg acggcgccgg
       61 gagatacgaa ctcactgacc gcaagggcgg cccttggccg tgctgtcggc caagtcgata
      121 aggcgggcgg cgagctcgcc attatcggtt ccctgataat atcggataat ccgatctctg
      181 ttcttcaagc taatccctca attactagtg ctgcggcgtg ctgctgccgt tgttgttatt
      241 gttgttgctg ttccaggcca ggggcggcgt attgaaatcg ccgtagtcgt cacgcttgcc
      301 gtggtcgacg gtgacattgc tcggtacgca aaggaagatt tccttgccga ccttctggat
      361 gttgccgtcc agggcatacg tagcgccgct gacgaaatcg acgacgcgtt tggcaatgtc
      421 gtccggcgtc gattcaaagt tgatgacgac gggtttgcgg tcgcggatat aatcggcgat
      481 gctctgggaa tcgtcaaagg tttccggttc gacgatgacc atgcgcagcg gtttttcctg
      541 gggtgctgca taattgacgc cggaacggcg cggggctaca gccggcgtgc ggcgcggtgc
      601 gcgttcttct tccatttctt cttcatcctc gtattcgtct gtttcaaatt cgtcgtcttc
      661 gttgacgccg cctacagtac ctactaattt atcccaaact ttctttactc ccatggtgaa
      721 tactcctcct tagtaattac ggggcccaaa gattgccgta cctacgcgga cgacattggc
      781 gccttcttcg attgctattt caaaatcatg ggtcatgccc atggacaggt aacggatttg
      841 accttccgga aacttcggct tcatgtcttc atacaaagcg tgggcaatgc ggaatacagg
      901 ccgtacatcc tctacgttgt cgtaattcgg ggcgatgcac atgaggccca ggacgcggac
      961 gtgttccatg gtcttggcca tttcgcacat agccgggaag tcttccactt ccatgccgaa
     1021 tttgcttcct tcgcgggcca cattgacctg caggagaata tcctgtactt tgccgatctt
     1081 gccggcttcc ttatccacag ctttgagcag gtgttcggaa tcgatcgagt ggatgaggtc
     1141 gaagtgttcg acggcatgct tggccttatt ggtctgcaaa tgaccgatta agtgccaggt
     1201 caaatggtta tccgggaacg cttccagctt ctgaagtgct tcctggaccc gattttcacc
     1261 gacgtcggtg acaccgcatt tagcggcttc ttccatcatt tctacgggat gattcttcgt
     1321 gactgcgaca agcgtaattt tgtcagtcct gccgacgcgg gcagctgcat cggcaattct
     1381 cttatttact gcctcaagat tttccttaat caagcctatc cctccgtagt gcatgtgatt
     1441 atatatattc atatatttat aaaacattac acttctttta ttatatatag ttaaggctat
     1501 aaaagctagt catatttaaa aatttgtccc atctgctttt caaaggacag caaaggcccc
     1561 catgcggccc gtatgaccgc cgtcgcggcg ataggaaaag aacttccctt cctcggcaaa
     1621 ggcacagctc gtcgtgactt cgatgtggtc cggccgaagg cccgcttcct ccaggagcag
     1681 ccggttggcc tgccacaagt caacggacca gacgccatct ttttcgccgg cgcagttccc
     1741 ataagccgtc cccaggcggc ggaaggccat ggccgtagac tcgctgacct cgtaatgggc
     1801 ccgggaaatg gaaggcccca cataggccag gaggtctttc gggtccgacc cgtaggccag
     1861 gcgcatggca aagaccgtct tggccaccag gcgctggacc gtaccgcgcc agccgtcgtg
     1921 gatgacggcg acggcccgct tcaccggatc gtagacgatg accggcacgc agtcggcgat
     1981 gcagagcatg agcggcacgc cggg
//
LOCUS       DAWCYS010000333         1997 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2696_length_1997_cov_5.587539, whole genome shotgun sequence.
ACCESSION   DAWCYS010000333 DAWCYS010000000
VERSION     DAWCYS010000333.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2696_length_1997_cov_5.587539"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          390 a          528 c          602 g          477 t
ORIGIN      
        1 ctttgatccc cgtttccacg atttccgttt ccgtcgcctg gtcctggaat ttcgggaccg
       61 gacgatggat aggccagtaa tcatcggctt cgactttagc gtcgacttta tcgacagtct
      121 ggccgaggac gttgaagata cggcccagga cacctttgcc gacggggacc ttgatgggac
      181 cgcccgtatt ttcagctgcc atgccgcgga cgaggccgtc cgtcgagtcc atggcaacgc
      241 agcggacgac gtcgtcgccc aagtgctgca tgacttctac aaccaggtcg atcttctggt
      301 cgccttcgcc accggtgata tggatggcgt tgtaaatggc gggcaaatct gcttccgagg
      361 caaaccgcac gtcgacgacc gggccgatga cctggactac tttcccaata ttattactca
      421 tgaagaggta ctcctctctt tcagaatgat cgccaggcgg cttactgcaa agcgttggcg
      481 ccgctgacaa tttccgtcaa ttcgttggtg ataccagcct gacggacttt attgtaggta
      541 acagtcaatt tgtcgatcag ttcgcctgcg ttgtccgtag ccgacgtcat ggccgtcata
      601 cgggcaccga gttcgctggc agcggactga aggaggctgt tgtaaatgaa cagatccaga
      661 tactgcggca gcaaggcgtc gaggacgagc ttcggatccg gcgcgaagag gtattcttcc
      721 cgcgggcccc cttcctgctg gccttccttc tgggtaatgg gcagcatctt gacgatgcgc
      781 gtatcctggg acagggccga cttgaagtgc gtgtaaatga catagacttc gtcgacttcg
      841 ccatcgagga acttctgcgt cgccaagtgg gctatctgct gggcgtggct gaactggggc
      901 ttgtcggaaa aaccgaacca cttcttttcc ggtaccaggt ggaggtgctt gaggtactcc
      961 aggggcttgc ggcctgcgac caggagcatc gatgcatcgc ggggcttgtc cttgaggacc
     1021 gacacgatgg ccttgttgac attggaattg aaagcaccgg ccagcccttt gtccgaaccg
     1081 atgatgatgt agcacgtctt cttgaccgta tcatgaggcg tgaacaaggc gttgcccgca
     1141 tcgggcgaag ccgcagacag acgctgcagc atgccttcca tcttttcggt atacggggcg
     1201 gtagccacgg cccgatcctg tgcacggcgc agtcgtacgg aggaaaccat cttcatggcc
     1261 ttagtgatct gctgggtatt ggtgacggat ttaatacgtc tgtttatttc gcgtgcactc
     1321 ggcatagctt attcctcctc tttgatcagt tcgtgagaag cgccgtactg cttggtgtat
     1381 tcagcgatag ccttctgcag cgtagcttct gcgtctttgc cgagttcctt cgtcgatttg
     1441 atgctttcgc cgacttccgg atagttactg tgcatgaact tgatgaagcc gtcagcaaac
     1501 tgagaaactt cttcaacctg tacaggcatg aggtagttct tgacggccag gtaaatctgc
     1561 atgacctggt cttcgacggg catcggctga tactgcggct ggatgaggag ctgcatcgtc
     1621 cgttcgccgg cgtcgatctg ggctttcgtg ctcttatcga ggtcggagcc gaactgggcg
     1681 aaagcggcca attcgcggta ctgggccagg ctcaggcgga gggtaccggc gattttcttc
     1741 atggccttga tctgggcgga gccgccgaca cgggaaacgg agaggccgac gttgatagcc
     1801 gggcggatcc cggagttgaa ggcttctgtt tccaggaaga tctggccgtc tgtgatggaa
     1861 atgacgttag tcgggatata ggccgacaag tcaccggcca gggtttcgat gatcggcagg
     1921 gccgtaatcg agccgccgcc gagttcatcg gacagcttgg cagcgcgttc gaggagacgg
     1981 gaatgtaagt agaatac
//
LOCUS       DAWCYS010000334         1980 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2724_length_1980_cov_3.776104, whole genome shotgun sequence.
ACCESSION   DAWCYS010000334 DAWCYS010000000
VERSION     DAWCYS010000334.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_1980_cov_3.776104"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          537 c          641 g          445 t
ORIGIN      
        1 gccttgagga gcgattcgtc ctgggccggc gtgatgcctt ccgtgcggag ataacaggga
       61 ccgttgccgt ccagcgtttc ccggaccgtg ccgtacaggc gctgtgacgt ggtcaggccg
      121 tatttctgtt catatacgtc accgcgggcg ttgacctgct tggccccgac gccctgggcg
      181 atagtccctg tcggcgcgat ggtatccttg cagcgcaggg cgatgaagcg catttcaaag
      241 gtcgtcattt cggccccgcc ccggatgccc atggcatagc cggcaccggt gttgaaggga
      301 cagtaccaca tcttgtgccg ggaaaaaccg ggattgttgg gccggtagag gccggctgcg
      361 ccgcctgtgg cacagaggac ggctttggcc cggatttcat aggccgtgcc cgtgcgcata
      421 tcgaagccca gggcgccggc catggtattg tcgcggacga tgtagtcggt aatattcaga
      481 tggttgatga cctggacgcc atcgagcttc ttgacggcgt cggccaggat ggtcttgata
      541 ttttcgccgt taatcttgat gttgcgcgtg ccgcgggcga cgtattctcc tttttcgtcc
      601 ttcaagatga ccagtcccag gtcttccagc ttcctggtga cttcgttgag gccttcggac
      661 atggtgaaga gcaagtcttc ccggacgatg ccggctgcgt ctttcttggc gtagtcgacg
      721 tagaactgcg gcgtatggcc cttggtgata taggcgttca gggcattgac gccggctgcc
      781 agacagccgc tgcggcggat atcggccttt tcggcgatga ccacggacgc atcagacagg
      841 ctgcggagag tcagggcggc ataacagccg gccgtcccgc cgccgatgat gaggacatcc
      901 gtatggatga tcttctcgct ggtaatctgc ataacataga ctccttcgtt aaatatagaa
      961 aacgtcagcc gaggtcatgg cgctgttttc ttttaaatac gtcttgtcgc cgtcgaggac
     1021 gtacaagcgg taaatgagga cctggacggt ctcgccgaca gacagtggcg ggtcgtcgaa
     1081 ggaatattcg ccgtggatgg ccaggccctt gatgcggacg gtcacgtcga ggtggctgcc
     1141 gcggaagacg acggcttcga ccgtcccttc ttcagcagcg ctgatatact gtttcatcgt
     1201 gccgtgtttg taaattttaa ggaactccgg tcggacgatg gcttgtttgc ccgctccgac
     1261 ttgcaggaat cccgtgagga tgttgcagtt gtcgatgaca actggagttc cgataaattg
     1321 agctacaaag ggcgtcctgg gctggcggta aatatccagc ggcgtcccga cctgttcgat
     1381 atggccgtgg ttggtgatga tgatttcatc ggccacttcg acggcttcgt cctggtcgtg
     1441 ggtgacgaag atgctggtaa tgccgacgcg gtgaatcgta tccttgagcc agttgcgcag
     1501 ttcctgacgg accttggcgt cgatggcggc aaaaggttca tccaggagca ggacctgggg
     1561 attcggcgcc agggcccggg caaaggccac gcgctggcgc tggccgccgg agagctggtt
     1621 gggatagcgc ttttccatgc ccgacaggcc gacgaggtcg atcatctcca atacgcgctg
     1681 gcgtatctcc tctttcggcc gcttctggat ttccaggccg aaggcgacgt tgtcgtagac
     1741 ggtcatatag cggaacaagg cgtagttctg gaagacgaag ccgatgcccc gcttggcagc
     1801 gggaatgtcg ttgacccggc ggccgtcgat gaagatgtcg ccgctgttgg gattctccag
     1861 gccggccagc atgcgcagga tcgtcgtctt gccgctgccg ctgggaccga ggagggcgac
     1921 gagcttgccc ttgtcgatgc caaaggaaac gtcgcgcgac gcgtggaagg acccataatt
//
LOCUS       DAWCYS010000335         1975 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2730_length_1975_cov_5.619792, whole genome shotgun sequence.
ACCESSION   DAWCYS010000335 DAWCYS010000000
VERSION     DAWCYS010000335.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_1975_cov_5.619792"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          453 a          496 c          595 g          431 t
ORIGIN      
        1 agggtcgtcg aggatgagga gcggccgcgg atggaacagg gaccgggcca gcgcgatacg
       61 ggcctgctgg ccgccggaca agcgctggcc gccttcgccg atgggcgtct ctatccccat
      121 cggaaaggta gcgcagtcct catccatgca gacctttttc aggaccgggg cgatgtcacc
      181 ggacaggccg aggcggatgt tctcttcgac ggtagccgca aagagttccg cgtcgtggcc
      241 gcagtagccg acgatgccgt ctggcgccgt gtggccgttg cccagttcct tggcgccaaa
      301 gaaaatctgg ccgccgtgcg ggttttcaca gaggaaggcc tggcccagcg tcgatttgcc
      361 gcaggcgaca ggcccggtga tgccgatgat agtaccggga cgggccgaga aggagaggtc
      421 ctggaaaata gggccgtcac tgccgggata ggtgaaggac aggtggcgga ccgtcagggc
      481 tgccggggct gccggcggta cgggatcggg cagttccgtt gccgcctgga gatggggctg
      541 gatgcgctgc cacgaaacct gggctttctg gatggcgttg aacaattttg ccgcatggga
      601 tgatttcgtc gccagcttga tgaaacaggc caggtacgtc gagaaagcgg cgatatccca
      661 gctttcccaa cccgttccca ggacgttgcg gccgccgaac cagatgacca gcatcgtgcc
      721 gatcatggcg atgactttgt aaatcggctg gagtgtattt tcccagagat tggccaggat
      781 catcttcttt tcatagtcag ccaggtagcc ttcataccgc tggtcctgta tggcttcttc
      841 gccgtagacg cggtacgtca gggcgttgct gatgcggtcc agggtcatgc ccgacagggc
      901 gccgctgctt tctttgctgc gggccgccgt cacggcgacc aggcggcgca ggctgttggc
      961 cagggcatag gcgatgggcg ggaagacgag gacgcacagc gtcaggcgcc agtcgtacca
     1021 gacgagcatg accacatagg ccgccatgac gacgccggta tcgaagattt ccgtcgtgaa
     1081 cttgcgcatc ccttcgacgc aggtgtcgat gtcggaaatg actttggtca tcaaggtacc
     1141 cgtatcggcc tgctccatgt cccgcttcgg cgtctggacc aggtgctggt aaagccggtc
     1201 tttcatggaa aggctgatgt tgttggcgaa cttccggacg tacaggcgct tgacatagcg
     1261 ggcgccctgg acgccgagga tgaccagggc gtaacagaga cagagggcgt acatatccgc
     1321 tgccgtccgg gtaccgccga aaatatcata gaggtattgg gccagctgcc cgtcgaacca
     1381 ggggccggca atcatgccga cattgtataa gataccgctc agcgtgacga tgagcagcaa
     1441 tttcttttca tggtaaaaat aataacggac cgaacccggg ccgaatcgtt tcaatgggaa
     1501 cacctcacat ttgatgatta tagttcatat atcatacact attatgggcc tttgtacaac
     1561 aggacgcagg cagggtgccg ttcatcgttc gtcgtaggac tatcgcatga agatttctgc
     1621 catcatgcaa ggtacctttc aatttatagt ttgtagcatg gagtttgtag aaaaccactc
     1681 actaaaaact acaaactgca aacttaaagc tcaaaaaggc gttgttttca tgtaacagcg
     1741 cctttttttc gaatgagact attgcctata tgatgggaaa tggggacagg cctcttgcgt
     1801 aaagtcaaaa agacgaatat aatagagttg tatgaaagtc aaaaaggaaa atatattttt
     1861 acggggtgat tatgatgagt gtattagcgg ataagattga acaattcatt ttacataaat
     1921 tcttagaaga acacaattca cacattgtat tgcgccgtaa tgagctggcc gaaga
//
LOCUS       DAWCYS010000336         1965 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2738_length_1965_cov_5.802094, whole genome shotgun sequence.
ACCESSION   DAWCYS010000336 DAWCYS010000000
VERSION     DAWCYS010000336.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2738_length_1965_cov_5.802094"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          395 a          509 c          525 g          536 t
ORIGIN      
        1 cataggcgtt cgagtcgctt ggagcctgga ccttgccatg gatggtattc ggatccacgg
       61 acggattata gctcttgatc tgcatgattt cctcctagta gtatttttcg agattgaccg
      121 gacggatacg cggtttggaa tcgtatttgg caatcgcctt atccaggttg actggtgtaa
      181 ttcccgtata cgtgtaggcc ggtgtgaaaa tgccggcttc ctgtttgaac cggttcgtat
      241 aggtatagtg gccgctgccg ttataatagg tgcccgggtt aatatcataa gcgccattgt
      301 acgtgccgct ggtcttgtcg gcggctccgt aggtccgatg gataccgtac atggacgcgg
      361 ccgtccctag aatcgtgccc cataaggcac tctttttttg agcttgtaag ttcgccgcgc
      421 tggtccggta ggcattggcc tggttctgat aattgacctc gttcacatgt tcggaccaaa
      481 cgtcattgcg ctggttctgc aataaggtac tgctgtcatc ccgccaggca tcatagctgg
      541 aagacagggc atccaaaggg gacccgctga gttgcagtcc gctggcgccg gcctgagcgg
      601 ctgtctgacc agcggctaat ttcatgcggt cattcagttt agactgctgt gctgcatatt
      661 gttcggcaat ctgttcttgt ttgacctggc tgatacgggc attttgttcg gctgccttag
      721 cctgtgcatt atacagtgca gattgtgcgt tatactgctg tttttgctgg ttgtactgat
      781 tgattccctg tatggccgta agggccatca tccacggtgc gccgcacatg gttcccactc
      841 ccttctatga aaaatagctg ccaggacatg tctttataga gaaagggctt cgagaattcc
      901 gcgccgaatg atttcagcca gcggcgggac ttctcgttgc tctgagttat catattccac
      961 attttcggat agcgccgctt ccagcgcggc aggatctgcc gccccagggc gacgaatact
     1021 ttacggtaat gctgatatag gtcaaagcgg gccacacacc acactacatg gccatagccg
     1081 caaatcacgc tgcggctgac gccgaagagc agcagcggcc gtccgtcgac gtaagcgata
     1141 tagttgtcgt aatctacatc gacggcgaag gtttccaggt ccgtttccgt tgcttttagt
     1201 tccatatcgt cccggtcccg caggcgtgct gccagccagc ggacgtcggg cagtaattct
     1261 ttcgttatcg gtttaaccgt tatatgcgct aaccaatccc ccatcaatac tgacctcctt
     1321 gattatggcg ttgagtttaa aggggtacgg ttcatcactc atgatacaca agtggttgcg
     1381 cgtgttggcc ccaatatcgt ataaagggac gctctgagtt aggtccccgg aaaataaggt
     1441 atattcgtcg gtgtatttga gttcatccat tttatcgaaa gtcaggccga ttttaccgcc
     1501 gtatgtatct tcgacgcgca gggtgaccgc gttgatttta tggacacgcc cctgtagggt
     1561 tccttcccgg aggctgactt ccatgccagg ctgttcaatc ttggtcatgt acggcaggcc
     1621 cgcgatgata cggctatacg actcatcgag atgcaccatg ccgtcggcag ggacaacttc
     1681 gtccttctga cggataccgt cacctacgat ggttaccgtt ttcccgatga ggtgcggcaa
     1741 agcaatcatc gtgccgctgc ccgtgacata agagtcggcg tattggtcgg tatcgtcgcg
     1801 catgacggca aactgctcca aataccgctt cggctgcccg ttcacggtcc gttcgacgat
     1861 agcgtagagt tcgtaattct cattgcgagg gatggcgaca atccatttat atttcccgtc
     1921 tgtaacgaaa tgggaccagg cgaatacttt ctgctctcgt ctcat
//
LOCUS       DAWCYS010000337         1964 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2739_length_1964_cov_4.665794, whole genome shotgun sequence.
ACCESSION   DAWCYS010000337 DAWCYS010000000
VERSION     DAWCYS010000337.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_1964_cov_4.665794"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          400 a          474 c          518 g          572 t
ORIGIN      
        1 tcattatcct tggccagttc ttcgttcttc ttcttttctt ccttgaaggc gtcgtcttcc
       61 aggtccaggt cgccgcggct gatggagtgg aagcggtggc cttcgtattc gccgatggct
      121 tcgacggcga attcatcgac cgggtcggtc atgtagagga cttcgatgcc gcggtcgcgg
      181 agcatttcca tctgcggcag gccttcgatg gcttctttat ccttgcccgt tgcgtagtag
      241 atcttctgct ggccgtcttt catgcggtcg acgtattctt tgatggtgca cagtttgcca
      301 tctttcgacg acatgaagag cagcaggtcc ttcaattttt cgacgttgtt ttcaccggtc
      361 atcataccgc tgtaaacgcc gattttcagg gatttgccgt attcagccca gaatttttcg
      421 tatttttcac ggtccttttc caggttgcgc ttcaacgtct tgaggatggt cttttccagg
      481 ttacggccga tgaggctcaa ttcgcggctc ttctgcaaca gttcacggga aatgttcaac
      541 gacaggtccg gcgaatcgac gaggcctttg acgaagcgca ggtaatccgg caggaggtcc
      601 ttgcatttat ccatgatgaa gacgtggcgc gaataaagct ggatgcccgg ttcataatcg
      661 gcatagtaga ggttgaacgg agccttgcct gggatgaaga gcagtgccgt gtaatcgacg
      721 gccccttcga cgcggttgtg gaagatttcc atcggttctt cccattcgta gaactgatgc
      781 tggaagaatt ccttatattc ttccggtttg atgtccgatt tattgcgcgt ccacaaaggc
      841 tgcatggagt tcagcgtccg cgtttccgta tgttcttctt ccggtgcgtc ttcgatgacc
      901 ttgccgtcgg cgtctttcgg tttttccttg gtcgtgaagt ccatggtgat cgggtagcgg
      961 atatagtcgg aatacttctt gaccaggctc tggagggtca tcttgtcggt atagttttct
     1021 tcactgtcgt cgccgtagaa gtccttgccc aggctgagga tgatcgtcgt gccgtgtttc
     1081 ggtttctgac attcttccag gctgtattca ccgtcgccag tggattccca tttataagcc
     1141 tgttcttcgc cggccttgcg ggtgacgacc gtgactttat cggcgaccat gaaggccgaa
     1201 tagaaaccga caccgaactg accgatgaga tccttatccg tgctgtcggc atcctgggat
     1261 tctttcaatt tttccaggaa tgccttggtg ccggactggg cgatagtacc gaggttttcc
     1321 atcaaatcct gtttattcat gcccaggccg ttatcggaga tcgtcaacgt gtgggcatcg
     1381 gcatcaggcg tcaaatggat ttcaaaggcc gtatcgcctt cgaggagatc gccattggtc
     1441 agggactggt aatgcagctt gtcgatggca tcggatgcat tggaaatcag ttcgcgaagg
     1501 aagatttcgt ggttcgtata aatagaatgg accatcaagt ccagtaattg tttcgtttca
     1561 gcttgaaatt catgggtttc ttttgccata ttcaaacact cctttgtacg attctgccgt
     1621 agtggcggat accttcaggt acccttacac caatatcata cagcaaaaag tcaaatagtc
     1681 aagtattttt taagaatttg acctttatcg tgcatccagt atgcaatttg aaatagtgta
     1741 ccagattgta ttatactata tttagtacgt tagtatgatt aaaagagata ggtgttcgca
     1801 gaccgcagag gctgtcctgc caaggcggcc cggctctcct gctaggagag ctgtcagcac
     1861 agctgactga gaggttattt cgcagtctgc ggtgccagat tatggggtta ccacaaacgg
     1921 gccgcccagc gggcggcccc tacgagccac aaatctcaaa ggag
//
LOCUS       DAWCYS010000338         1960 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2744_length_1960_cov_7.044094, whole genome shotgun sequence.
ACCESSION   DAWCYS010000338 DAWCYS010000000
VERSION     DAWCYS010000338.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2744_length_1960_cov_7.044094"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          444 c          467 g          490 t
ORIGIN      
        1 ttaagttttc cagaaccgtc ctttttctgt tgtatcatgg aggtatggat atatctgctc
       61 gttctgtttc atattataat aaagaaaaga atggtgaatc tgttgaaaga aaaattgact
      121 gccaacgaaa tccgctgtca taggtatgat tgtattacga ccatgtgctg tccgtcatac
      181 attccgacta tacataggat tcacggcggg acgcccgcca ctgctgtccg ttctggcagc
      241 cgccctgcac ctcccgatag tcggtacctg gcggaccaga aaggctctgg gcgtccccta
      301 cggtatcgca tacaggcgat gggagagaaa gaggtcacag attacaaaaa aatacacggc
      361 cacggccttg tgcgtagggg ccgaccttta acctctcaga ccgcctttgg cggccagctc
      421 ccctataaag ggagccttgt gcgtcccgtt gcgagatttc atcacagcct tttcgagtat
      481 aatggcaggc ttttacgagc cacgaataac gagccacggc cgttgccacg agccgagggt
      541 tactcgaggc ggccggtggg gccgtcggat tcccacttga tgaactagaa gaaaaaaagc
      601 ttgacaaggc ctgtctaaat ctgtaaaata aataagtaag cttttcactt ggactccatt
      661 agccccggag aaagagcacc aaaagcttgg tcagttagtt aaattcaggg aggaatcaca
      721 cagcatgaaa actacattta tggccaatgc aggcaacatc gaacgcaaat ggtttgttgt
      781 tgatgctgaa ggccagacat taggccgtct tgctgccgaa gtagctaaag ttcttcgggg
      841 aaaacataaa cctactttta ccccgcatgt tgatacaggc gattatgtaa tcgtagtaaa
      901 cgcagcaaag gttaaattaa caggtaaaaa attaatccag aaaacctatt tccatcattc
      961 cggttatccc ggtggagcta aattcactca ggccggccac atgctcgaag cacgtccgga
     1021 acgcgttgtt gaaatggcag ttcgcggtat gctcccgaaa aataaattgg gtgaacagat
     1081 gtatcgtaaa ctgaacgttt atgcaggtgc tgaacatccg catcaggctc agaaacctga
     1141 agttttgaag ttaaacattc gctaattaat accgggagga agatttcatg gcagtagcac
     1201 agtactacgg cactggccgt agaaagacat ccgttgctag agttcgtctg gttccgggtc
     1261 agggtaaaat cacagtcaac aaaaaagaat tgaacgaata tttcggtcgt aaaacactgg
     1321 aattggttat taaacagccg ctcgtactca caggcgtaac tgaacagtac gacgtcatcg
     1381 ctgacgttaa gggtggcggc ccgtccggcc aggccggcgc aatccgtcac ggcatcagcc
     1441 gtgccctttt ggaagtcgac ggcgatttcc gtcaggctct caaaaaagct ggtttcctca
     1501 cacgcgaccc gcgtgaaaaa gaacgccgta aatacggcct caagaaagct cgtaaggctt
     1561 cccagttctc gaaacgttaa tattttttca agatattttc gtcaaaccta tgccattaaa
     1621 agaggctgtc gcatgtgcga cagcctcttt ttgagtatca agtttgcagt ttgtagttgt
     1681 tagtgaaggc ttttaaattt aaaaccattt tctacaaact acaaactccg tactaaaaac
     1741 tataaaataa aagaggctgt cttcatatga cagccccttt ttgaacccgt cccgccacta
     1801 gcccaagcgg cctaaagatg ttaaaatgga taggaaataa gctgtttgct gtgtgaaggg
     1861 ggaatctgca tgtttacgta ttatatgccg acgaaggtat ttttttgaaa gtgctgtatc
     1921 gcaaattcag tccaatggaa tgatgatctg ggatggaagg
//
LOCUS       DAWCYS010000339         1957 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2750_length_1957_cov_4.722397, whole genome shotgun sequence.
ACCESSION   DAWCYS010000339 DAWCYS010000000
VERSION     DAWCYS010000339.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2750_length_1957_cov_4.722397"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          564 a          506 c          392 g          495 t
ORIGIN      
        1 atctttactg atttcatcat atatattgcc ttcacgattg atggccgata tgctttctcc
       61 acgtaaaaga gatatcaaat cctgtagctt ttcgtcagac agcgacagca tcagctgatt
      121 caaaattgaa ttccccgatg tattaatcca ataatcatcc aaaacttttt gatcgaaaaa
      181 attgatgacc gaccatggat tataaatatc ctgatcccca aaatgatagc catcgtacca
      241 agcccggata tctgataaag ccccttccat cgccaggtcc tgcgccattt tctttatttc
      301 atatggggta aatcccatga catcagcata catccgagtc ataacagaac atacttttaa
      361 attattgagg ccgctgaaaa tactttcttt agcaattcgt aatacccctg tcaaaacagc
      421 aaaaaccagg gatgcattgc tttttaaagc gttgttgaac caaatccgga aataagaaat
      481 cgctttatca taaaaaccgt gatcgtaagc acattgaatc ggtgcatcat attcatcaat
      541 caggataatg gtttttctgt cataatagcg gcgtaaatag tgggataagt ataacaagct
      601 gacctgataa tctgcttcac ttccggtcaa atcaaggatg cgtctataat acgcctgttc
      661 agacggtttc aataagctgc ccttcagtag atagtcatgc cgttcatatt ctgactgcat
      721 caataaacaa aaggaatcat ataaagattg ccaagtatca ttctgtacgc ctttcagcgt
      781 caaaaaaatg acaggatact ggccgcgatg cttcatatat ccagcaccat ctgaagcaat
      841 atgcaatcct tggaagagct cccggctttc cttttctttt tcagctgaaa agaaccagtc
      901 cagcatactc attgtcaagg ttttcccgaa acgccgtggc cgtatcaata aggtaacttc
      961 accatgacta tcaatcaggt ccttaataaa atccgtcttg tcgacgaaat aatagccttc
     1021 gcgaactttc tgaaaatcat ctattcctac aggcatacgg tcattcatca cattcgcctc
     1081 cattccgcgc catcatttct tatattctaa caaaattctg cacattttcc aattctggcc
     1141 cgccgaccct ttctctgact gtgacatcgt ggtatgattt tgatgatagg attacgaaag
     1201 gagttatcat catgaccaat acagaaaaag aacggcaaaa agccattacg acggccggca
     1261 atccggccag accgaccggg gaagcggggc agcagatgct ggcgcgcatg aatgagagcc
     1321 atgccgccct tacggcatgg ggcctgtccc atttccattg gcgcggcgat gaaacggtcc
     1381 tggacatcgg ctgcggcggc ggagccaatc tccatcgcat gagcgcccac atcacgacgg
     1441 gccatctgac tggcgtggac tattcagcga cgtcggtcca gacctcacgc cagacgaatg
     1501 ctgacgatat agctgccgga aaaatgacag tctgcgaagg ttctgtcgaa gccctgccct
     1561 ttaccgacca aacctttgac aaaatcacga ccgtcgaaag tttctatttc tggcccaatc
     1621 cgccggaaaa cctgaaggaa atccatcgcg tcctgaaacc aggcggcacg ttcctgctca
     1681 tcgccgaaat ctacggccac gacggcctca gcgctgacgt ccgggaaaac atcgaacgct
     1741 accacctcta taacccgacg ccggaaacct ttgaaaccat tttccatgat gccggcttct
     1801 cctccgtcca ggtccacatc gaaaaagacc accgctggat ctgcgtcgaa gggcggtaaa
     1861 aaaagaggct gtcgcattaa gacagcctct ttttgagtat tatataatgg gccccttgtc
     1921 aagaccacgt tctaaaattt taagagtggc agtaagc
//
LOCUS       DAWCYS010000340         1952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2758_length_1952_cov_5.009489, whole genome shotgun sequence.
ACCESSION   DAWCYS010000340 DAWCYS010000000
VERSION     DAWCYS010000340.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_1952_cov_5.009489"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          388 a          533 c          586 g          445 t
ORIGIN      
        1 cctcattgta atagtaaaaa tgcgtatttt tttctattat tgtatgaaat cgccagaaat
       61 gctatagatt catcagaatt gcttgaaata gcccatttct ctcctgccgt aattgacaac
      121 atttataagg ttatgttaca tcgtcagcaa tggaagaagt aacactgagg gcggttccgg
      181 acgtatcctg aagggcgcca tggccatgaa gtttctcaga atgcttcttt cattttttat
      241 tttgcactgt aagggtcgcc acgtggggcg cccgttcctg cccgatgtgg gaatgtactc
      301 acatcccgga cgggctgaac aggcaggtgc gattccccac gattatacgg gatcgacatc
      361 gatgaggacg ccggtctgcc agcaggccgg gatgcgctgc atggcttttt tgatggctgt
      421 caggtctttt ccggtcaggg acaggaccag gcggtattta ttgcgtatct tcttgatgac
      481 gtcttcgtaa ggaccgatga cggcgacttc atcgccgtgg tccccgttcc agctgttcag
      541 ggccgctgcg acgtcgttgg ccttgcgcca gacttgttct tctgattcat cctggactaa
      601 gatcttggcc atttcctgga aaggcggata gcccaattct ttccggaact ggatttcctg
      661 gtcataaaag gcccggtaat cctggcgggc agcgcactgg atgacgtaat ggtccggcgc
      721 gtaggtctgc atgacgacgc gccccgggat atcaccgcgg ccagccctgc cagccgcctg
      781 ggtcaggagc tggaaggtcc gttccccagc ccagtaggcc gggatattga gcaggctgtc
      841 ggccgataag atgccgacgg cgctgacgcc ggggaaatcg tggcctttgg cgaccatctg
      901 agtccccagg aggatgtcat attcatgacg gcggaaagct tcgatgatac ggtcgccgct
      961 gtgcttgcgg ctggtcgtat cctggtcgag gcggatgatg cgcgccgacg gcaggagttc
     1021 gtggagctgc tgttccactt tttccgtgcc gctgccaaag aatttgatgt atttgctgcc
     1081 acatgacgga cagaccgtcg gcacgggctt ttcggcatcg cagtaatggc agcgcaggtg
     1141 ggcggcctga cgatggtaga ccatggccac gtcgcacgta tcgcatttga cgacgtagcc
     1201 gcacttgcgg cacataacga atgtcgagaa gccgcggcgg ttgagaagga tgatggcctg
     1261 ttgccgggct gccagtgtct cttccaggag attcaataag caatcggaaa cgacgcggta
     1321 attcccgcgg gccaattcat cgcgcatgtc gacgacctgg accgaaggca ggggccgctg
     1381 gccgacgcgc tctgtcagct cgaccaggac ataatcgccc tgcagggcct tatagtagtc
     1441 gcccagcgcc ggcgtggcac tgccaagaat tacggggcag ccgtagtacg ccgcccgctg
     1501 gcgggccacg gcgacggcat gatagcgcgg cgcttcgtcc tgcttatacg aactgtcatg
     1561 ggcttcgtcg acgatgaaca ggccgatgga ctgggccgcc gtaaagccag ccgaccgggc
     1621 accgatacag atatgactcg tcccgttctg catacgcagc cagttgttac gcttctcacc
     1681 ctgggaaagc tggctgtgca tgacgacgac atcgtcgccg aagcggcgca ggaaacggcg
     1741 gacgatttgc ccggtcaggg cgatctccgg cactaaaacg acgacctgcc ggccttcttt
     1801 caggacctgg ctggccagct gcatgtagac ttcggtcttg ccactgccgg tgatgccgtg
     1861 gaggacgtag gtttttcctt gttcatcgct tccgataact ttaactgcct ggcgttgttc
     1921 cggtgtcagc gtaaagggag catcgccaga aa
//
LOCUS       DAWCYS010000341         1932 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2795_length_1932_cov_4.778903, whole genome shotgun sequence.
ACCESSION   DAWCYS010000341 DAWCYS010000000
VERSION     DAWCYS010000341.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_1932_cov_4.778903"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          395 a          564 c          503 g          470 t
ORIGIN      
        1 tgcggttgaa cagcttgtgt ctgatgggag acagctgggg gagagtcgga gtctggatct
       61 tgacgaagag cttgcccgta taagccggac ggatccaggt cagcgtatcg tcatcgcctt
      121 caaagcggac gtccgtgcag tctgcgacca ggccgacgcc gagctgagcc gacaggcgac
      181 cgccgaggtc acggccatcg ggcgaaccgc cgatgaggat gacgctgggt tcatattttt
      241 cagccaaagc ggcgatagct ttggtgtaca agacggaatc gtaggcagcc agggcactgt
      301 cttcgacagc gacgacggca tcagcgccat aggaaatggc ttcctggctg gctttggccg
      361 tatcggcacc ggcgacgaag gcgacgacct gtttgctctt ggcatcggcc agctgacggg
      421 ctttacccaa caattcgatg ctcagcgggc tcagggcacc gccggcgatt tctatatata
      481 cgcagatgtt ctgtttcaat tccataattc agccctcctt acagcaagtt cttggcagtc
      541 agttctttga cgagattagc cgcagcgact gccggcgtag ccccttcgat catgacgccg
      601 accggattcg gttccggtgc gaagagttcc gtgactttcg tcgccgaacc agatttcccg
      661 acacttgctg catcgaggcc caggtcttcc actttccaga catcgaagac ggcttttttg
      721 gcagccatct tgccgcggat cttcggagaa cgcggcgtat tgctcttttc cgtaaccgag
      781 acgacagccg ggagctgagc ctgtaccgtt tcaatgccgt cgtggtttag gcgcgtagcc
      841 gttaccgtcg tgccgtcgac actgaccgaa ttggcatagg aaatgagggc catacccaaa
      901 cgctgggcaa tagccggcgg gatctgcccc gtatcgccat ccgtcgagcg cttgcccgtg
      961 aagaccaggt cggcaccgcc caatttttca atggccttgg ccaataccag gcctgttgcc
     1021 agcgtatcgg agccagccat gcgttcgtcc gagacgagga cacctttatc ggcaccgata
     1081 gcaatgccat cacgcatcat ttcaccagcc agggcgttgc ccatggtcag gatcgtcacc
     1141 gtaccaccga tggcatcctt gacggctacg gccgcttcga tggcgtgttt atcaaccggg
     1201 ttgagcatcg acggagcgcc gacacggacc aagttgttgt ctttggcgat ttcgatatca
     1261 gaaatcgcag gaacctgctt taccaaaact acaatattca taagtaagcc tccttttttc
     1321 gtcttcttat gtcacctatt atactccata ttcaacaatt tatcaacgga taattatttt
     1381 tatttacaga atctatatct aacgtatctt cccattgaca gccctaggct attcttttaa
     1441 aattaaatag tattaaacag gttaatcgtt tgattttcca gctaatcttt atacttttag
     1501 gaggttagga ggtttttatc atgtctgaat cccatccgga gcggcgccct tatgatccgc
     1561 ccatgtctcc ggaccagaat tacgttgaaa tcaatgctta tgtcgtcggc tgggggctgt
     1621 tcttcgccgc cctcttctcc ctggccgtcg gctatctctg cctgaaaatc ggccagaccg
     1681 tcgatgcctt tgcgcccgtc tccgtcctgg ccatgggcat ggccgtcctc ttcaagcgca
     1741 agaatgcctt cccggaaacg gtccacatcc aggccatcgc cagtgccggg accaacatca
     1801 tcggcggcgt catgttcatc ctgccggccc tgttcatctt gcagctcgac agccagctga
     1861 cctatacgga aatggtcctc cccatcgtcc tcggcggcgt cctgggcgtc ctcctggcga
     1921 cgacgttccg cc
//
LOCUS       DAWCYS010000342         1923 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2810_length_1923_cov_4.858137, whole genome shotgun sequence.
ACCESSION   DAWCYS010000342 DAWCYS010000000
VERSION     DAWCYS010000342.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_1923_cov_4.858137"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          547 c          559 g          479 t
ORIGIN      
        1 gactgcttcg actgcgtcgc cgtctgcccg aaaggggccc tgactttttc ccgggccttt
       61 ccggtcaagg acccggaaaa aacgccatca ggcgagggcc tcagccggcg ggaaatgctg
      121 gtgacgacgg ccgttgccgt cgggaccgtc gccgctggcc tggcccgcag tcagaccgtc
      181 gtgccggcct tgctggggac ggggaaaggc aagaagaaaa tcttgccgcc tggggccgtc
      241 tcggccgacg cctttgtcca gcgctgtacg gcctgccatc tctgcgtcag ccgctgccct
      301 agcggcgtcc tgtccccggc gtcttagaga acggcacctt gcagatgggc cagccctaca
      361 tggatttctc taagggctac tgtgtctaca attgcaattt ctgttctcaa gcctgtccgg
      421 ccggcgctat ccgtccgctg gacctggctg tgaaggagca gacgaagatc ggcatcgccc
      481 gttatgtcca gttccactgc ctcatcgccc gcgacggcat cgtctgcggc aactgcgccc
      541 gccactgccc ggtccaggcc attaccctgg tcgagaaccg ggacggccgc agctatcctc
      601 atgtcgatgg ggcgcgctgt atcggctgcg gttcctgcga atatcactgt ccggccaaac
      661 cggcggccat ttccgtcagt cccttgccga aagcctgagg cgtcagtctt ccgggtccgt
      721 agcaatgtcc ggttcgttct tgcggttgaa gccgatgatg cggcgcggct tttcgatgtc
      781 gtcgtggcgg gggacggcga cgaggagcaa gttgagctgg gaaatgtgct ggaccagttc
      841 gaccgccgag ccgtcggaca attcgccgta gaagacgatc atgttcgggt cccagtagcc
      901 gatgtcctgg atgctgaagg tgatgtcgcc agccgatacc aggcgcccgc cggcctgcat
      961 gtcgtcgggc aggcccgatt caaagttgtt gatcagggcg acgatgcgct gatacatgtc
     1021 ggaagccaga tagggcgctt ccgggtcgtc ttcttcttcc gggacggcgc cgggctggag
     1081 gatagtcagt tccttttctg cagcggcctc tttttcgggg gccttttgtt ttttcttatc
     1141 gtttacgtct gccataaaaa tccctcctta tcattctcat tatatcattt agacatagaa
     1201 tgaggcaatt cctgctactt ttttccgggt agcaggcttt ttttcttggc atccctatgc
     1261 tgatatggta taatggtcaa gacatgctga catcaggtaa catattttca cacgaaaaga
     1321 ggttttttct tcatggaata tgtgaacgag ttggacacta tatcggcggg agctgcgaaa
     1381 aaagaggcgg ctctcaaggt acatcgtctt acgtttatgg aggctacgat gatggtcgtc
     1441 gggtctacca taggttccgg tgttttaggt ttagcttttg ccagtcgcaa tgccggctgg
     1501 cccgtcctgc ttacgtggct tgtcgtagca ggcttttttt ctatcatctc catgctctat
     1561 gtcgccgaag cgacgctgcg caccacgagg cccttacagc tgtcgggcct ggctgaaaag
     1621 tacgtcggca agatcggttc ctggctgatt ttcttttccg tcggggccac gtcgttttgc
     1681 agcctcatcg cctataccag cggctgcggc aagatattga gcgaattctt cggcatttcc
     1741 ctggaaatgg ccagctgcct ctttgccctc ggggcgacga gcgtcgtctg gctgggcctc
     1801 aaggcaaccg gggtagcaga gaaatatttg agttcagata tgacggtcat gcttattctc
     1861 ctcgtcggcg cgtcactttt ttcggcccga gtacctgtct cagatatcct ctatacccac
     1921 tgg
//
LOCUS       DAWCYS010000343         1915 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2826_length_1915_cov_5.204839, whole genome shotgun sequence.
ACCESSION   DAWCYS010000343 DAWCYS010000000
VERSION     DAWCYS010000343.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2826_length_1915_cov_5.204839"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    816..825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          502 a          524 c          416 g          463 t
ORIGIN      
        1 ctgcacatct acggcatcga cgcaccggcc ttctgctatg ccgatacact ggaagtcgcc
       61 aagaaactct atcacttccc gtcgaaccag ctcgacgtca tctgcgatga aatggatatc
      121 cagaccgaag aaggcaacct gttgagccag gccatcgcca taggccgctt ctttctcctg
      181 gcccacaagg aataccccat gttcctgccc gacctgacgt acgtccaaaa agcaccgacg
      241 acagacgaca aagaagccgc cctcatcgcc gccgtcgaac gcgaagaagg aacgccgaaa
      301 gaaatgttcg ctcccggaac accggacccg gccctcatcg acgacctggt acaaaaaggc
      361 tacgtagaag ccggcgaaaa accaggcaca tactacgcca ccgacaaagg cctggacttc
      421 tctgaaggac ggtaagttag tggctagtgg ttcgtggctc gttggtcgtg gctcgtgggt
      481 cgtgacatga atgtttgacg aaaggctccc attatagggg agctggccgc cgaaggcggt
      541 ctgagaggtt gcctttaaca tcaaacaaaa aaaggggact atcgcatgca gtctcatatc
      601 accatgcgta gtcccttgtt ttataaaatc atcagctagc cactaactgc taacccccaa
      661 aaaaaggcgc tgtcttcatg cgacagcgcc tttttgttac catcaaacag ctcttatttg
      721 gagtacaatt cgacgataag tgtttcgttg atttctaccg gaatttcgtc gcgttcgggc
      781 atacgggtaa gtgtgccttc gaagccgtcg aagttnnnnn nnnnnttctt ttcgatgtac
      841 ggtacctcga aagtcttcaa ttcctggaaa gattttttgt acatttcgtt ggaacgggaa
      901 gcttctttca aagcgataac ggaaccaact ttaacgtgtg ccgacgggat gtcgcagcgt
      961 ttgccattga ccaggatgtg gccgtggttg accatctgac gtgcctggcg gatggagttt
     1021 gcaaaaccga tacggtatac caggttatcc aaacggcatt ccagcaattt cagcatgttc
     1081 tggccagtaa cgccttccat cttcttggct ttatcataat aacggacaaa ctgacgttcg
     1141 agcaaattgt agtatgcgcg aagtttctgt ttttccaata actgtgtgcc atattcagac
     1201 atttttttct gacgctgatc tttacgtacg cgttttaatg ccttagggtg tccgtacaca
     1261 ttaactccga accggcgaca ttccttaaaa cgcgggtctc tcctcgttgc cattttctac
     1321 atcacctcat attagtaatt caagaaatta aattttgctt acgcaatgta taaataatat
     1381 catgaaaaaa gccttgctgt caaggggtca tgattcttga cttcttttct tcactatggt
     1441 atactttagt caataaacaa ctaataatta ctatcaatta ggaaatgatt tgtaaattca
     1501 ggaggtgctc ttatgaccaa cttcatcaca gccgtcagca accggcgcac gaactacacc
     1561 ctcagcaaag acgtcacgat tccccaggaa accattacca agactatcga agacgtcatc
     1621 cgcgaagtac cgtcggcctt caacatgcag tccggcaaag ccgtcatcgc ctttggcgaa
     1681 acgcacgacg ccatctggaa aatcaccatg gatacgctcc gcaaaatcgt cccgccagcc
     1741 aacttcgcca cgacggaagc caagatcaac tccttcgccg cagcctatgg cacgatcctc
     1801 tacttcgacg acacgaccat cgtcgacaac atggcctccc agttcccgct ctacgccaag
     1861 aacttcccca tctgggccca gcagtcaaac cgaatcctgc agttcgccgt ctaga
//
LOCUS       DAWCYS010000344         1914 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2829_length_1914_cov_4.560516, whole genome shotgun sequence.
ACCESSION   DAWCYS010000344 DAWCYS010000000
VERSION     DAWCYS010000344.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_1914_cov_4.560516"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          488 a          454 c          517 g          455 t
ORIGIN      
        1 gatacgagca ggccgatatt gccgctgcgg ataatggcca tgggattggc gacaatgacc
       61 aggccgatga tactcaagac caaggtaatg gccttcttgc gggtaaaggc ttcgtgaatg
      121 atgaaatacg aaaaaatcat gacaaaaatc ggggtactgg agaaaacgat agctgccagg
      181 ccggcattgg ccatgttgac cccgatctgg aagaggatca tgctgaagca gatgttaatc
      241 agcccgagga cagccaaata gccccaatca gaacgatcga ggtggatgct gcgcttcttc
      301 aaatcgcgga cagccagcgg catgagcagc agcccgccga cgaggaaccg aaggaaagtc
      361 agctggaaag gcgtgaacgc cgttccggca atcttcaagg aaatttccat cgtaccaaag
      421 gctatggctg tcaaaacaat acataatgta cccattttag atgacataca gaaagacccc
      481 tttcaaaatg gttataaaaa cacgctttat tatataccta ttgagaaagg atagcaataa
      541 aaaaggagct gtcgcagtgc aacagttcct ttttgagtat gaagtttgca gtttttagtt
      601 tttagtgaag gttttttaat ttaaagccct cttctacaaa ctacaaaaag aaaagacccc
      661 gcatgagggt cgaaaccttc atgcggggcc tttcttctat tctcatgcag cgagtgcaat
      721 caaattagtc ttcaaaaccc ttctgtaaac gtacatagga ttcgcgacga gcttttgcgt
      781 ctgcttctgt cttagcatag agagcgtctg cttcttccgg gaagcctttc ttaagggaag
      841 cataacgaac ttcaccctgt aagaattcct ggaatttgct gtaatccggt tctttggagt
      901 cgaggctgaa cggattcttg ccctgagctt tgagatccgg gttgtagcgg aagagatccc
      961 agtaaccgca ttcgacagcg agtttttctt cgagctggct cttgcccatg cctttacgga
     1021 tgccgtggtt aatgcaagga gcgtagcaga taaccaggga cgggccatgg taagcttcag
     1081 cttcagtcat ggctttcatg agctggttct tatcagcacc catagctacc tgagctacat
     1141 agacatagcc ataggagata gccatcatgc cgaggtcctt cttcttcgtg cgtttgccgg
     1201 cagcagcgaa ctgagcgata gctgcagtcg gggtagcttt agaggactga ccgccagtgt
     1261 tggagtaaac ttcggtatcg aggacgagga cgttgacgtc gtggtcggaa gcgaggacgt
     1321 ggtcaacacc gccgtaaccg atatcgtagg accagccgtc accaccgatg atccactggc
     1381 tacgtttaac gaagaactgt ttcttagcga ggaagtcttc gagaaccggt ttgccagcag
     1441 cttctttttc gaggagagct gtcagtttgt cagcgcgttc acgagagcct tcgccttcgt
     1501 tcaggttagc agcccagtcg ctgagtgcag cctggagttc cggagaagca acggctttag
     1561 cagcgtcgag gttttcagcc aagtctttgc ggactttgtc tacgccgagg aacatgccga
     1621 gaccaaattc agcagcatct tcgaagaggc tgtttgccca agccgggcca tggccagcag
     1681 cgttcatggt gtacggggaa accggagcgg atgcacccca aatggaggag cagccagtag
     1741 cgttagcaat catcatgcga tcgccgaaga gctgcgtgat gagtttgaca tacggtgttt
     1801 caccgcagcc tgcgcatgcg ccggagaatt cgaggagcgg agtttcgcac tgagaagcga
     1861 tgccggtctg cttgttctgc gggttagctt tcggagcatc cgtttccgta ccat
//
LOCUS       DAWCYS010000345         1913 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2833_length_1913_cov_5.213671, whole genome shotgun sequence.
ACCESSION   DAWCYS010000345 DAWCYS010000000
VERSION     DAWCYS010000345.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2833_length_1913_cov_5.213671"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          358 a          520 c          553 g          482 t
ORIGIN      
        1 gtgcgtggat acggtagacc gacgggtttt cgctgtcccg gagatagcag gctacggttt
       61 cattggcaat gagcatgctt tcttcgacga tctgttcggc aatggtccgt tcccgttttt
      121 ccagacgcag cggtacgccg tccggatcaa ggatgacttt gtattcaggg aaatcgaagt
      181 cgatggcccc gcggcggagg cgcatgttct tgaggatcgt cgacaaggcc cgcagttcgt
      241 ggagcatggg catgaagggc tgcaagtcgt caggcacgat gccttccagg agggccttgc
      301 ggatttcgac gtagttgcag cgccgtttga cgcggatgac ggccgggccg atgtcggcct
      361 tgaggacctt gccttcttcg tcgatttcca tgacgcaggt catggagtaa cggtcttcat
      421 tggcgttgag gctgcagatg ccgttggaca ggaccgtcgg cagcatgggg atgacccggt
      481 cgacaaggta gacgctggtg ccgcggttat atgcttcctg gtcgatggcc gatcccgggc
      541 ggacgtagta gctgacgtcg gcgatgtaga cgcccagtcg gaaatggccg ttgggcaggc
      601 ggacgacgtc gatggcatcg tccaggtcct tggcatcttc actgtcgatg gtgatgagct
      661 ggcggtcgcg gtaatcgcgg cgcccttctt ccaaggtgac cgtcgtgtcg atcttggcgg
      721 cttccttctt gacgtcgtcc gggaagtcga aaggcaggcc gtggcgggcc atgatgacct
      781 tgatatccag gtccttgtcg ccgtcatagc cgaggacttc cgtcacttcc ccttcggctt
      841 tgcgcccttc ttcgggccac ttggtgatct tgaccaggac gcgggcactg ctgcgggcgc
      901 cgccggtctt gtcgaggggc acgtagacgt cttcgcggat gcgttcgtcg tccggcgtga
      961 cgaaggcgct gtgtttttcc agctggaacg taccgaccag ggtcttgttg acccgttcga
     1021 cgatggatac gatttcccct tcctgtttat gacggccatc gccgctgtgc aggatgcgga
     1081 ccgtgacctt atcgttattc atggccgtat ggcggttctg ttcggcaatg tatacgtcct
     1141 ccgtcaggtc ctggctgagg acgaacccga aggtcggcgt ataaccttta taaataccgg
     1201 tgatttcctg gggctgtttg gccgagatat accggtctcc tgcgattctc atgagctttt
     1261 cactcctcac taattcttca aatgtgtcaa atacgtcagc cagccgggcg ccggtgatgc
     1321 cgagctgttc ggcacagtct tcgaccgttg cgccgccgct gtcttcatta catatactta
     1381 ttatatcatg agccagctgt aaatccatag ttcccctcct caaatcgccg ttctgcaaag
     1441 gataaaaaag gagccgccgc atatgcgaca gcccctaaga attcattaaa aagtatgcat
     1501 cagtctgcct aacagcaatg tacaaatggc aaatgctatc gacaggacga tagtcagttt
     1561 ggaaagcagg gcatccatgc cgcgggcccg gctcccgaac aagtcttcgg caccgccgcc
     1621 gaaaccggcg cccatgccct gggtcttgct cttctgtgcc acgacgacac cgatgatgag
     1681 cagtgccaaa atgacaacga tgatttctaa tgctgtgatc acgttaatcc tcctcacaca
     1741 atgacgctaa cagtactatt gtacactcat ttagcttttc tatcaatagg ttctttggct
     1801 ttctcatcgg gttccgtttc tttttctgca ttgcgcgttt ccagataatg ttccagcttg
     1861 cgtttgaccc gctgcagggc attgtcgatg gacttgacgt gccggtgcag ctg
//
LOCUS       DAWCYS010000346         1913 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2832_length_1913_cov_6.906351, whole genome shotgun sequence.
ACCESSION   DAWCYS010000346 DAWCYS010000000
VERSION     DAWCYS010000346.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2832_length_1913_cov_6.906351"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          513 a          473 c          507 g          420 t
ORIGIN      
        1 cccccagtaa aaaagacatc aaaatagccg tcaggaccgt ccgtaccact ttatcgtcgc
       61 ggcgggcacc gagatactgg gcaatggtga cattagcccc cagggaaatc cccatgagca
      121 gcatgaccag gatgccgatg acaggcatat tgttgccgac agcagccatg gcgctcttgc
      181 cgacaaaacg tcccaaaata aagacgtcgg cactgttgaa gagctgttca aataagccca
      241 tgaggccgat cggcatggca aaaaagaaca acttgtcggc aatagagccg tgcagcatat
      301 ccatgccgcg gcggccctta ttttgtgtat tttccataat tatgattacc tctcgttctc
      361 gtgaatgatg ctttcttccc ttagtatagc ataaaagagc ttgccgcaca tgcgacaagc
      421 ccttttaagt ggttagtggc tagtggttag tggttagcgg atggctaaaa atttgaaatc
      481 atcttctaca aactccgtac tacaaactat atatgtcaat cgtggcggat gaggagctgg
      541 atgttttctt ttttataggt cgtatagatg cttttgatga actggtgctt gaggacggct
      601 tcgtcgacga tgttgcggat acgcaggacg actttgaagg aaatgccgta ttcccccatc
      661 tggtcatagc ggacgagggg ctcgaagccg gtgacgccgt attcactgtg gtcgaggatg
      721 tgccgggcca cgccgagggt gacgtcttcg acacgctgca ggtcattgtc ataggtaatg
      781 gtcaagggaa tggaaatggt acattctgcc aaaggctgtt cgtagttggt cagcgtcgcc
      841 gtggcaatgc tcttgttggg gacgatgacc atattccccg tcgccgtgcg aatcgtcgta
      901 ttacgccagt tcatatcgac gacgcggccg gcttcgccgc tggacaggcg gatgtaatcg
      961 cccatgtgga cctgtttcga taccagcgtc gtaatgccgg aaaagaggtt ggccagtgta
     1021 tcctgcaagg ccagggccga tgccaggccg ccggcgctga gagctgtcag gagcggcgaa
     1081 atggaaatgc cataggaact gaggatgaaa aggatgccca tcagagtcgg catgaaacgg
     1141 aagatattgc gccagctgtc gcgcgggatt ttcgtcacgc ggctcagcac ttcgatgacg
     1201 atccggaaga cgccgtgcag gatgacaatg ccaatgaaga tgaataaaaa aaagctagga
     1261 aatgaacgtg cggcccgcca tattccattt cataggaaaa agtattcaca atggattcca
     1321 taaaaaaagc ctcctccgaa gcaataaatc taaaataata atgatttatt atactccgga
     1381 gaaggtacta ccacaatatg gcaacggtta cagtttcatg tcattttggg catgaaacct
     1441 ttgttgtaag gccctttcaa aagtcgtata ctaaagaaaa tccataccct aggaggaaac
     1501 ccatggaaat acggacactg caatacttcc tggccgtcgc cagggaacag aatatgaccg
     1561 gtgcagccaa tatgctgcat atcacacagc cgacgttgtc ccggcagatg gccgatctgg
     1621 aaaaggaatt gggcaagcag cttttcatcc ggaccaaccg cagtacactg ctcacggaag
     1681 aaggcatccg cctgcgccag cgggccgagg aaatcgtgtc cctcgtccag cagacgaaag
     1741 ctgaaatcag cgacgaccag ccggaactga cgggctgtat ccgcatcggt gccggcgaaa
     1801 caccagtcat gcacttccta accgacacct ttgccaaact gcagcggaaa catccgggca
     1861 ttacgtgtga actctttacg ggtaacgccg atacggtcga agagaaactc tcc
//
LOCUS       DAWCYS010000347         1911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2838_length_1911_cov_5.656789, whole genome shotgun sequence.
ACCESSION   DAWCYS010000347 DAWCYS010000000
VERSION     DAWCYS010000347.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_1911_cov_5.656789"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          570 a          409 c          376 g          556 t
ORIGIN      
        1 gtttttctct ttgtattaat attatatatt gatacatatc aaaccgctat cacttttacc
       61 ccccctgtat atttttagtc atattataca tgaattatat tcatttatat ttttgtacta
      121 aaggctgcca ttaattgaca gccttacacc tacttcacca gttctttttc catagtttga
      181 aaggccggca catagtagcg aataatacgc cgaacttttt cctccactgg ttcacaacaa
      241 tcgggatgat atcgatcctt aagcatcgac agccaagtag attcactaac aaataaatat
      301 cgttcataag catctaaaag taattctttc ggtgtcaggg aaatagcaag gtcaggatct
      361 ttattttcaa ggcgttcccg aaatacctgt tggcctaaat caaattcgtc cataaaccag
      421 tgaacaatcc catgtaatac aaagacattc gacaaatctt cttttttcag accagaaaaa
      481 tccttcaacc cttgggaaaa taattcaaac gttttcatat tatcaccacc tttttatctt
      541 tttcttttct ataatattat cgtataaaat aaatacacaa agtgcaaaag agttaaattc
      601 attctagtca tcgcttttat tcattaattt tatattaaac tgaaaaaagg actcgaatcc
      661 ttgacaggat tatttgatac atgaaatact atttattgat gttcgaattt atcataatgg
      721 gggaattttt catgtatgta aaaccaaaaa cgttatccat attgctagca gccacatttg
      781 ccgcttccat ggcctgggga acggctccaa aaaatgtttc ccagaccttc gctacaaaag
      841 cggaagcgtc ggtaaaggcg ccgcttcagg cgaaagcgcc ggcttataca ggaaaggcat
      901 tacagaacac tcgcaacggc atggttcagg gatatgtaca aaatcaggct ctaatctgga
      961 aaggaattcc gtaggggcag gcagaacgct ggaaagctcc gacagacgtc aaggcctgga
     1021 atggcatctt cgatgcgacg aaaccgggac ctgttgccat tcagggagcc atgggcaaaa
     1081 caaaaggcag tgaagactgc ctgaatctcg atattttcca tcctaataca aaagaaaaaa
     1141 atctgcccgt cctgttcttt atccatggtg gtaataatca gagcggtgct gctgaccaag
     1201 tgaactggca gaagtttgct gaaagggaac acgtcgtcgc catcagtatc aatcaccgtc
     1261 tgggtgcact gggctttaat cccctaccag cattaaagca tggtacggct gaagaaaatt
     1321 ctggtaactt tgctgtattg gattttgtga gaggcttgga ctggacgaaa gaaaatattg
     1381 ccagcttcgg cggaaatgct aacaatatta cgattgcagg cttttcttcc ggtggacgtg
     1441 acgtcatggc attgcttatt tctcctatag caaaagggaa attccagaaa gcgatttcct
     1501 tcagtggcgg tatgactact tctgatacgt cctgggctca gcagattttc gcccagcatt
     1561 ttgcttctct cgtcgttaca gacggcatca aaaagagcga catagatgcc gccaaatggc
     1621 tgttgcagga tacaccggag gtccgtgatt ttttacagaa tctatccggc gaccgtattg
     1681 ccgctgcatt tggtcatgct ggtatccgca tgagtgcctt ccctcatctc tataatgatg
     1741 gcatcgtatt agaaaaagat gcttttgcta cgaaaaatta caatcaagta ccgctcatca
     1801 tgacgactgg tacagatgaa ttctccctat atacgcgaac tgatagttac ttttctctat
     1861 aatgggcctg tgaattcaga ccagtactga aaattttaag agtgcatgat a
//
LOCUS       DAWCYS010000348         1905 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2855_length_1905_cov_6.646486, whole genome shotgun sequence.
ACCESSION   DAWCYS010000348 DAWCYS010000000
VERSION     DAWCYS010000348.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_1905_cov_6.646486"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          448 a          558 c          523 g          376 t
ORIGIN      
        1 aagaaattct ccgcaagcac cgcattgaaa aattacccct tgtcgataaa gaaggcaccc
       61 tccagggcct catcaccatc aaggatatcg aaaaggctac aaaatacccg aattctgcca
      121 aagatggcaa cggccgtctc ctggtcgctg ctgctgtcgg cgtaacccat gacatgaccg
      181 accgtctcga cgctctcgtc gctgccaaag tcgacgtcgt cgtcatcgat acggctcacg
      241 gccattccct gggcgtcctc aatacgctga aagaaatcaa gaagatgtac ccgcatctcc
      301 cggtcatcgc cggcaacgtc gctacgggtg ccgctacgga agccctcatc gaatgcggcg
      361 tcgacgccgt caaagtcggc atcggccctg gctccatctg cacgacccgt atcgtcgctg
      421 gtatcggcgt accgcagatc acggccgtct atgaatgtgc taaagtcgct cagcgctatg
      481 gtatccccat catcgccgac ggcggcatca aatattccgg tgatatggcc aaagccatcg
      541 ctgccggcgg caacgtcgtc atgatgggca acctcctggc cggcacggaa gaaagcccgg
      601 gcgaaacggt catctatcag ggccgcagct acaaagaata ccgcggcatg ggttccctgg
      661 ccgctatgga atgtggcagt aaggaccgtt acttccagga agatgccaag aagctcgtcc
      721 ccgaaggcat cgaaggccgt gtaccgtaca aaggaccggc agctgacacg atttaccaga
      781 tggtcggcgg tctccgcgca tccatgggct actgtggctg ccacaacatt aaagaaatga
      841 ttgaaaatac gcagttcatc cagatcaccg cagccggtct tcgtgaaagc catcctcacg
      901 atgtcaacat cacgaaagaa gctccgaact acagcgtaaa ctaagcaggg cggagggata
      961 cgttgattag tacaatcgat atcttgtcca tccccatatg gaacgtcact atggaagaag
     1021 cccggaacct ggcctttaag tgcatcgacg aaggccgggc cgcttccatt gcgacggcca
     1081 atgcagaaat ggtcatgcgg gcccagacgg accgcgaact ggcccatatc ctccagcatg
     1141 ccgacctggt agttcccgat ggggccggcg tcctgtgggc tgcagaacag cagggcaaga
     1201 aatttaaaga acgcgtgaca ggtgtcgatt tggcgtgcag tctgctccaa gaagcagccg
     1261 cacgccaaac ggctgtctac ctctttggcg gtgccccggg cattgccgaa caggctgcgg
     1321 ccaatgtgca gcagcaactg gggtccctga acatcgtcgg gactcattcg ggattctttt
     1381 cggctgacga agaacagggt atcattgaag atatccgcag caaggggacg cgcatcctct
     1441 tggtcgccct cggcgtgccg aaacaggaaa aatggatatc tgagcatctc tacgaactcg
     1501 gcccctgcgt ctgtatgggc gtcggcggga ccttcgacgt cctggctggc aaagccggcc
     1561 gggctccgaa atggatgcag gacaaccgcc tggaatggct ctatcgcctg ttgaaggagc
     1621 cgacgcggtt caaacgcatg ctggcgctgc cgaaattcgt cgcggctgtc aagctgggcg
     1681 gccggcctaa gtgatgaggt gatgagttga aaagtgatgt cttgaacaaa ccttatatcg
     1741 tagaagacgg ataccccttc attatcgtgc ctctgttact ggctgtggca gcaggctatt
     1801 tcatcaatgc ctacgccgtg gttccgctgc tgctgctggc tgcgtattac acctatttct
     1861 tccccaaccc cagacggaac atgccgacag aagaccatct cctcg
//
LOCUS       DAWCYS010000349         1904 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2858_length_1904_cov_4.326663, whole genome shotgun sequence.
ACCESSION   DAWCYS010000349 DAWCYS010000000
VERSION     DAWCYS010000349.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_1904_cov_4.326663"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          446 a          446 c          533 g          479 t
ORIGIN      
        1 cgttattaga atattgtacg gacgcattag cctcagccat cgcacgcggc gtcagagact
       61 acgaatgtag cttacggtag gaggtaatga atcatgagca aatggacaag ttttagagac
      121 agcatggaat ccgcattaga cattgcggac gtcacggaag ccgcaaaaga acaggtaatt
      181 gcaagcatca caggggatgg catggacgcc atttccgccg ttgcggataa attcgtcgca
      241 cagattcagg cccaggcgga cacggaacag ggctggaata tggtacgcga taagttcgtc
      301 ctgcctttgc tgatcaacag catcctctgg gcgattaagc tcgtattaag caagagtacg
      361 acggccagca ctcagcccgc cgcacagccg gaagcaacaa cgactgacgg ccagcagtag
      421 caatttcata ataatgtatt ataagggcgt cgcgttttgc gacgtccttt tttattgcca
      481 tttttatttt atacgtataa aactattgac attatacgta tgtatatgct atagcaggaa
      541 aaggcgaatg cgattgtaaa gaaatatagc aaaaaaagac ggccgataat gaggccgtct
      601 ttttcatgcg gaaatgcacg atgcaaaata atccggatag aattgtaaca ttatgttcgc
      661 attaactttt gtatggtgca ccaaaaaaat agtgaaaagg accccgcacg aaggcttttg
      721 ccatcatgcg gggtcctttt tcattttata gtttgtataa tctggtttta aattgttaac
      781 catccgctaa ctgctgcatg gtagggaata cacaaaattc acagcgggac gccctctggg
      841 cgtcccctac agcaaaaaag ggattgtcgc atgtgcgaca atcccttttc tatatcttac
      901 actaaacctt gttcaatcat gacgtcggcg actttcatga aggccgtgat gttggcaccg
      961 gcgacgtagt tgtcggggac gccgtatttt gcagcgttgt cacgggattc ttcaaagatg
     1021 ttgcgcataa tgtctttgag ggtagcgtcg actttttcaa agggccagga caggcgttcc
     1081 gagttctgag ccatttccag ggccgatacg gcgacgccgc cggcatttgc ggctttggcc
     1141 ggcgcaaaga ggacgccgtg ttcctggaag taagccatag cgtcgagggt cgacggcatg
     1201 ttggcgcctt cgccgatggc ttttacgccg ttctggacga ggattttagc cgattccagg
     1261 tcgatttcgc cctgtgtagc gcaggggagg gcgatgaagg ccgggatttt ccacatatcg
     1321 cggctgtttt cgtggtattc tgcatccgga tgggtttcga tgtattcttt gatgcggccg
     1381 cggcggactt ctttgatgtc cttgacggtt gcgacgtcga tgccattggc gtcatagata
     1441 tagccgctgg agtcggagca ggtgacgacc ttggcaccca gggcctgggc tttttcgatg
     1501 gcatagatgg cgacgttgcc cgaaccggaa acgatgaccg tcttgccttt gatgtcgatg
     1561 ttcttgttgt ccagcatatc cttgacgaaa tagaggagac catagccggt agcttctgta
     1621 cgggcgaagc ttccgccgta atcaatgcct ttgcctgtga gtacgccggc atcgaaagcg
     1681 tcctggatgc gtttgtactg gccatagagg aagccgattt cacgggcgcc gacgccgatg
     1741 tcgccagccg gtacgtcgat gttggggccg atgtggcggc acagttcggt catgaagctc
     1801 tggcagaaat gcatgacttc ccggtccgat ttgcctttcg ggtcgaagtc cgagccgcct
     1861 ttgccgccgc cgatgggaag gccggtcagg gcgttcttga agat
//
LOCUS       DAWCYS010000350         1895 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2876_length_1895_cov_5.365761, whole genome shotgun sequence.
ACCESSION   DAWCYS010000350 DAWCYS010000000
VERSION     DAWCYS010000350.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_1895_cov_5.365761"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          385 a          486 c          497 g          527 t
ORIGIN      
        1 atcgcctcaa gggcgccact agggctgttt tttggcgact tggcttttct ccttcgattt
       61 cagtgaaaaa gactccctta tcgtccccaa tgccataccg ctcttcttga tttccagtat
      121 tttcagactt tcgaggcagt gcatattgaa taacacgtta tcccaatacg tttcccgaac
      181 ggcccatgcg tcctttaatc tgatgatact ggcactcttc catttgtctg ccgtatgcag
      241 gctttccagc ggccgggtat tgaagagata atggtacagc tcatcaacgg gtgtcgtgat
      301 agtggatacc ttgtcgttga tttcggacgc tctataatat ctgagtgcgg tatcgaatat
      361 gattccaggt tttccatgcc ttctgtcgta ttcttccagc agccagacgg tttccttatt
      421 cgtcgcgctg tacgccgtct tgccaaaagc atcaagcgtc cggctggcac ggctgtcaga
      481 tagggtgaat gaggtatcat cgagcttgta cagcgtgaac gttttggcaa tatccatggg
      541 cgttacgaca aggtgaactg gaaggtcgtc gtcatggtat cgtcagcggc cttgttgatg
      601 acgtcgaata cgacgcggtc gaggaacgtg ccaccgcttg cggcgttgca gataccggct
      661 tccgtgatgg caccggtagc ttccccggcg tcaaaggtcg tcgtcagcgt gaagagtttc
      721 gtgccggaac tatgagcgta gctggccgct ttccgcttga tttccgtaac caatgcagtc
      781 tgtgtagcgt caacggcggt cgtgcctgtg ccaactgccg tataccccat gacggccggg
      841 cgcgtcgggt cggccatagc ggcgcagata tagtcaaacc cgccgttcaa gataaggttg
      901 tctttatggc gggtttctac gtcgccgttg gcgtgatgga tgacaacatt caaagagcct
      961 ttgattttca tttcgtcttt attcatgtgc ttttactcct ttgggttaaa gaaaatacgg
     1021 tcaaatgcac tgcatggcgg aatgaacgcc ttgatgtatt tggtcgtatc gaggtcgaat
     1081 tcgcggatga agaaaagccg cttgtcggca ctctgtgcaa gtccgaaaaa cagccagtcc
     1141 ctatctgcgg tatcaatttg taagctgagt atccgttcat tgacagtatc ccgtacgtag
     1201 aacgagtccg tccgcttgtc atacccgatg tacagcgtaa tatcgcgcca tatgccgtca
     1261 gcgcctaaat gctgtgcttc gtagctgtcg acggtgaagg tcatgtcctt gacggcgata
     1321 tagtctgtta ggttcggctt gttcccgcgc atctctgcaa tgaagcagtc tgtgagcggg
     1381 gccgtcttct tgaaccagaa tcggaatgag actgtttcgg ggatactgca cgagtattcc
     1441 agctggccca cgtcggtgat gagtgcgccg tcgttccagc gtaccggggc atagcttgta
     1501 ttcttctccg tcagcagtgt gccgcccgtg gccttactcg tgccgtcgat gatagcatca
     1561 aaggcatcat cggccttgcc ggtataccgg gctatctgct ttttaagctc tacgccgtca
     1621 aggtcgccaa ggatgccgca taccaggata tgcgacgcct cgtagctgtc gacggtgaag
     1681 gtcatgtcgc atacccttaa cgtctgctcc gtgacggcat tgattttgca gtcaatccag
     1741 ttacgggcct ttatcttctg cggcaacgat actttcatga ggtattcgcc gttaaaggat
     1801 tccttctcca gccgcaaacc ttccatggcc gcgttatagt acatattcgt ctttatcccg
     1861 ctatagccca gcttgtattg gttatagtcg aggat
//
LOCUS       DAWCYS010000351         1893 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2880_length_1893_cov_4.056583, whole genome shotgun sequence.
ACCESSION   DAWCYS010000351 DAWCYS010000000
VERSION     DAWCYS010000351.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_1893_cov_4.056583"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          506 c          578 g          459 t
ORIGIN      
        1 gttcatgatg cgcgtgactt tgccgacgta ggtcttgccg acttcgacgt cttcggtcag
       61 tttttcgatc cattctttgg ccttctggcc gctttcgccg tcaacggcag cgatgtatac
      121 caggcccgaa ttgtcgatgt cgatcttggc gcctgtttct tcggtgatct gcttgatcat
      181 cttgccgccc ggcccgatga cgtcgcggat cttatccggc ttgatgtgca tggagatgat
      241 gcgcggagcg tagggagaga gctcctttcg cggttcactg atgacctgca tcatcttgcc
      301 catgatgtgc atacggcctt cgtgggcctg tttcagagcc tgggccagga tttcacggct
      361 gaggcctttg atcttgatat ccatctggat cgccgtgacc cctttcatgg taccggcgac
      421 tttgaagtcc atgtcgccca aggcgtcttc catgccctgg atatcggtca ggatagtgaa
      481 gtcatcgcct ttggtgacca ggcccatagc gacaccggca acgggcgctt tgatgggcac
      541 gccggcatcc atcagggcca gggtgctgcc gcaaacgctg cccatggagc tggagccgtt
      601 cgattcgagg acttccgata cgacgcggat ggcgtacggg aattcttctt cactgggcat
      661 gacctgggtc aatgcgcgtt ctgccagttt accgtggccg atttcacgac ggccgggacc
      721 gcgggacgat ttcgtttcgc cgacgcagaa ggacgggaaa ttgtagtggt gcatgtagcg
      781 gcggtctgtt tccaggccga cgccgtcgat gtgctggcat tccgacagcg gagccagggt
      841 gcagaagctc atgatctgcg tctggccgcg ggtgaacagg cctgtgccgt gagtgcgggc
      901 aaagaggccg acttcgcagc tgaccggacg gacttcgttg acacggcggc cgtcaggacg
      961 gatcttgtcg acggcgatca tgtggcggac gatttccttg atcatcttct gcgtgacttc
     1021 agcgacatca tcggcgttgt ccggatagat gtcggcaaag tgttcataaa tgtcggcttc
     1081 gacagcgtcc atcttttctt cgcgttcgac tttgtcagcc gtgaagatag ccgtcttcat
     1141 ggcttcttcg ccataagcgt ggacagcttc gacgatatct gccggcggtt ctttttcgac
     1201 gaattcctgt ttcggtacgg aaatcttgtc gaggaagctg agctggaatt cgacgacttt
     1261 cttgatttct tcatgggcga acatgatggc gtccaggacc tgttcttccg ggacttcgtg
     1321 agcgccgcct tcaaccatga ggatggcgtc ttttgtaccg gcaacgacga tatccatgtc
     1381 gctctgttca ctctgggcga cggtcgggtt gatgatgaac tggccatcga cgcggccgac
     1441 gcggacaccg gcaatcgggc cggcaaaggg gatatgggac atgcacagcg atacggatgc
     1501 gccgatcata gcggcgattt ccggagcgtt gtcatggtct acgcagaaat cataagccac
     1561 gacgtggacg tcatgacggt atgtcttcgg gaagagcggg cggatcggac ggtcgatgag
     1621 gcgggcaaaa agggttgccg attcaggcgg tttgccttcg cgtttgatga agccgccggg
     1681 aatcttgccg acggagtaca tcttttcttc gtaatcgaca gtgagcggga aaaagtcagc
     1741 gtcttcacgc gcttccttcg agcccgtagc cgtgacgaaa acggccgagt cgcaataacg
     1801 gaccaatgag gcaccgctcg cctgtttagc cactttgccg acttcaatca ccagcggggg
     1861 gccggcgaaa tagatatgga atttcggttc ttc
//
LOCUS       DAWCYS010000352         1883 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2902_length_1883_cov_5.363786, whole genome shotgun sequence.
ACCESSION   DAWCYS010000352 DAWCYS010000000
VERSION     DAWCYS010000352.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_1883_cov_5.363786"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          415 a          491 c          525 g          452 t
ORIGIN      
        1 gttcaaaggg gatgacatat ttatcgaaga gggcgacgaa ccagcggata ggacggacga
       61 agtggaagtc caggctgccc cagtgcatgc tcttggggaa attaagaccc gtgacgatgc
      121 ccttcatgag gtccggcaag aggccggctg tgtcagcgcc gacgctggtg acgttggcat
      181 agacgtagcc gtcttcaacg acgaggtcct tgacgtcgat tttctgaccc cgggcaaagc
      241 ccatggcagc tttcgtcggg ttaccgtcgg catcgtaagc gatcttgacc gacgggcctt
      301 tatgtttggc cgatacgtcg gcctgttttt cagcgacgcc cttcatgatg agggcgatgc
      361 ggcgcggcgt accgagggtg cggatcgatt cataggcgat atgggattcg ccaaaggatt
      421 tcccggccag gtcccggacc tgggaaagga tactcggcat ataatgagcc gggatttctt
      481 cagtaccgat ttcaagtaac aaatctttgc tcatcttatt tttcctcctt attcagcatc
      541 gggaaaccta atttttcacg ctgttccaag tacgctttgg cgcaggcccg tgccatgttg
      601 cggacgcggc cgatgaaggc agcccgttcg ctgacgctga tggcgccgcg ggcgtcgagg
      661 aggttgaacg tgtgcgagca tttcaatacg tagtcatagg ccggaaggac gaagccggct
      721 ttgatgaccc gcatggcttc tttttcgtat tcatcgaaga ggtggaacaa gaggtcggcg
      781 ttgctcaatt caaaggcata cgtcgactgt tcgacttcgt tctgatggaa gatgtcgcca
      841 taagtgacgt cttcattcca tttgaggtca tagacgtttt cgacgccctg gatatacatg
      901 gccaggcgtt ccataccgta tgtgatttcc gacgaaacag gagcgacgtc gatgccgccg
      961 acctgctgga agtaggtgaa ctgcgtgact tccatgccgt cgagccagac ttcccagccc
     1021 aggccccagg cgccgagggt cggcgattcc cagttgtctt cgacgaaacg gatatcgtgt
     1081 tctgccgggt tgatgcccaa cgtttccaga ctcttcagat agagttcctg gatgttgtcc
     1141 ggcgacggtt tgcaaatgac ctggaactga tgatgctggt acaggcggtt cgggttgtcg
     1201 ccatagcggc cgtcagccgg gcggcgagac ggttcgacat aggcaacgtg ccacggttcc
     1261 ggcccgagcg tgcgcaggaa ggtagccggg ttcatggtcc ctgccccttt ttctacatca
     1321 tacggttcgt agatgataca gccctgagaa gaccaaaagc tcttcaatgc aaatatcatg
     1381 tcttgaaaat tcatcgtatt ccctcctaaa tacaaaaaag tccctgtaac acatgacatg
     1441 ttacagggac gagtatacgt acccgcggtt ccaccctgat tggtccaaag acccgcttga
     1501 ttcaccggcc atacacatcg aaaagacttc cggccgcggc cttcagagcg ccttccgcat
     1561 cggcagcagg ctcacactgt ccctgcttcg ctgaggatga tacgtactta ctctcctgca
     1621 tcgcctattg atttgtacaa accttatcat agcagagatg gggggaaaag tcaataaaaa
     1681 ggagccgtct cgtagagaca gctccttttg agtttgcagt ttgtagtttg cagtaatcaa
     1741 tactttgaat ggattcacta caaactctat actaaaaact ttaaactcac tatcattgct
     1801 tgccgtattt ttcggcgacg tccaggcggc gcagggctcg ttgcagggcc gcttcggcgc
     1861 gggtcacgtc gaggtcggca gcg
//
LOCUS       DAWCYS010000353         1872 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2917_length_1872_cov_4.332966, whole genome shotgun sequence.
ACCESSION   DAWCYS010000353 DAWCYS010000000
VERSION     DAWCYS010000353.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2917_length_1872_cov_4.332966"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          533 c          528 g          464 t
ORIGIN      
        1 gttcctttta ggagtttcac aagttgcggt tccttatagt gtcctctttg tcagtatctt
       61 cctgttcgtc gtcattcctc tggcaggcgg tatcctgacc cggatgactg tcgtcaaacg
      121 aaaaggtctc gattattttg aacagcactt tgtacacaaa ttcgattcgg caacgacgtg
      181 gggactgttg ctgacgctgg tcatcatttt cagctcccaa gcccaggtca tcttatcgaa
      241 tccactccac atcgtcctca tcgccattcc ccttaccttg cagaccttct ttatcttctt
      301 ccttgcctat ggagcctgcc ggctgctgaa actgcccttc gacattgccg caccggccgg
      361 gttcatcggc gcttctaact tctttgaatt ggccgttgcc gtagccattg cgctctttgg
      421 cgtcaccagc ccggcagcct tggcgacgac cgtcggtgtc ctgaccgaag tcccgattat
      481 gctgaccctg gtccgcattg ccttagcaac taaaaactag ctacgctagt ccctggaatt
      541 gtaggggctt gctgcagcaa gcccgctcca gaatatgtgt gctttcccaa tcagttttct
      601 ggagaaatcg tccccatatc caacgggcgc cccctctact gcggtccgtt tcagcaaccg
      661 tcctgccatt ccttacagtc atgacctgac gggctgaaaa ggcacgtggt cgcccctaca
      721 aataaaaccc catccgctaa ccacgaacca cgagccacta aaaagcggct gtcttcaaag
      781 gacagccgct ttttcctact tcccttcgta ataataccgc gtccacatga cgacttccga
      841 cagggacggg tgaggggtca tggtttcgct gaaggtgtag accgatttgg gattgagggt
      901 ccgcgtcagc ggtttggccc gcagggcctg gaccgtcggc gacgcgatgt cttcgtggat
      961 gggatggacg acgtgggtgc cgctcatgag gttattgatg aaaggctgga tcaggatgca
     1021 tgcttcgggg ccgacgatgt gggcgccgag gatgaatttc gtcttcttgt cgatgaccaa
     1081 tttgacgaag ccgtcgtctt catcgcccgg ttcgtagccc atggcgtagc ccttggccga
     1141 cgatgaatag tggttgatgg ccgtttcgac gtcatagcct ttcttcacgg cctggtcttc
     1201 cgtcaggccg acgtgagccg cttcgggata ggtgtaggtg acggccggca cggcatcgta
     1261 atcggcccag cgccacgttt ccggttcgtt tccggaaaag aggttgtgtg ccaggatttc
     1321 cgcttcgtaa ttggccttgt ggcggaaggg ggccaggccg ttgacgtcgc ccagggccca
     1381 gatattttca gccgtcgttt ccaggaattc attcgtttcg atgtagccgc gcttgtccag
     1441 gcggacgtcg gtattttcca ggtgcagcag ttccgtcatg ggccggatgc ccggagcgac
     1501 caggatttct tcggcagcca cttcgccttc ttcgcccgtc gtgcggtcgc ggaaggacag
     1561 gaccttcaag ccgtcgcgct ggctgacgga aaccgtgtct ttattgaggc ggatatccat
     1621 gccgtagcgc tggaactgct tgaggatgaa tgccgatacg gccgggtctt ccttgggcag
     1681 gaggcggaca ttgtgctgga ccatggtgac cttcgtgccg gcggcgtcga agacgtgggc
     1741 aaactcacag ccgatagggc cgccaccgat gataatcaga ctctggtagg gcttcttcgg
     1801 atacgacggg ccgaagaacg attccgacgt caaatagccc gcttcttcca ggcccggcag
     1861 ggaggggata tt
//
LOCUS       DAWCYS010000354         1871 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2918_length_1871_cov_5.582048, whole genome shotgun sequence.
ACCESSION   DAWCYS010000354 DAWCYS010000000
VERSION     DAWCYS010000354.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_1871_cov_5.582048"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          461 a          509 c          458 g          443 t
ORIGIN      
        1 acacgctggc gttccatgat ggcaaggtat ggcgcaacgg ccagccttta gatgcaccgt
       61 acatcaatga accgatggaa tacagccgca aaggggaaat caagatcccc gaaggctatg
      121 tcttctgcat gggtgataac cgcaaccaca gcagtgacag ccgcttcatc ggcccggtcc
      181 ccatcgacca cgtcttaggc cacgtcatct ggtaggcctc aaaagagcat acgacagttc
      241 gcaatccatc ctgtcggtaa acaaaactag gaagcattac aatggtatct tgcaatgcag
      301 acgggattgt ttcggcaacc ccgtcttttt ttcttttctt tggaaggagg ttacgcctat
      361 gtataatttt ctcgcgaata cgaaacgcat gaccgtcgct gccatggtca tggccctgta
      421 catcgtcatc ttgtacgtca ctcaggggat ttcctttggc ccctatcaga tccgcatcgc
      481 cacgtcgctc tatgcattgg cctttgtata tcccttcctg gtggtcccct tggggcttgc
      541 gaacttcatt gccaatttcc tcttcggcgg cctgggcttt atcgacatga tcggcggctg
      601 cttcgtcggc atggccacga catggctcat cgtccagatc cgtcagcgtc agtatagtcc
      661 atggctggct gccctgccca tctggtgggt gccggccctc tgcgtcagcg cctggctgtc
      721 gtacctgctc gatttgccct acgccgtcct ggtcagcagc ctggctgtcg gtcagctgcc
      781 gccggccatc tgcggggtcc tgctcatcca ggccctgagc catcgttatg tcttgaagaa
      841 ggagttttga tatgagccga agtaaagaag aactgcaggg cgtgacccag ctgggcaatc
      901 aacacacgac gtacgcccag gactacgacc cgtccctgtt ggaaacattt gtcaacaagc
      961 atccagaccg ggattatttc gtcaagttta actgtcccga attcaccagt ctctgcccca
     1021 tgacgggcca gccggactat gcgacgattt acatttccta cgtccctggt gagcgcatgg
     1081 tagaaagcaa atcgctgaaa ctctatctgt tcagcttccg caatcacggt gatttccatg
     1141 aagactgcgt caacatcatc atggacgacc tcatccaggc catggacccg aaatatatcg
     1201 aagtatgggg gaaattcctg ccccgcggcg gcatcagcat cgatccgtac tgcaactacg
     1261 gccggcccgg cacgccctgg gctgacgtgg cctggcagcg cctgtcccag cacgatttat
     1321 atcctgaaaa ggtagataat cgctaaatct attacctttt acggctgttt catgatataa
     1381 taagtctaaa aacaacagaa gtaaggggtg ttacctgtga aattgaaaaa aatgcttttg
     1441 gccctgggat tctccctggc cttgtcggca tcggtcttcg cagcggataa tatgtggcaa
     1501 tataaccagt atacgtccat cgacatgaac tcggccaagc tctattacga tatgggccgc
     1561 cagatcctgt cctttgagac ggtggaacaa tccggtgaaa cgaccgacta ctgcacctat
     1621 gtgtataaca tcgatgacca gacgatccag ctgaaaaaga tgattaccaa gacgaagaag
     1681 cataagtata agagcagctt ctatccggaa tccatcaaag acggcaacgc cgtcatcaag
     1741 gccagagccc gcatggccaa tgatgtattg gtcaaagtaa aggaaaagga tgagaaaaaa
     1801 tagtgtggga gttggcagtt ttcagagaaa acctttaaat ttaaagccat ccgctaacaa
     1861 ctaactgcta g
//
LOCUS       DAWCYS010000355         1867 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2929_length_1867_cov_5.643488, whole genome shotgun sequence.
ACCESSION   DAWCYS010000355 DAWCYS010000000
VERSION     DAWCYS010000355.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2929_length_1867_cov_5.643488"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          435 a          525 c          514 g          393 t
ORIGIN      
        1 gcgtataagg ctgacggcag ggctgggcac aggcaccgcg gttgccgctg cggccgccga
       61 ggaaactgct catcaggcac tggccggaat aggccatgca cgacgcgccg tggatgaaga
      121 cttcaatggg aatggacgac tgggagcaga tgaagcggat ttcctgcgcc gacaattcgc
      181 gggacaagac ggcctgagtg aagccattgg cctcaaggaa acggacaccg tccaagtcgg
      241 ccacggtcat ctgcgtactg ccgtggagcg gcagatccgg tgcgactttc cgggccagag
      301 cggctacggc caggtcctgg acgatgatgc catcgacgcc gaatgagtcg aggagctgca
      361 aataggccga caaagcctct aattccgtgt ctgccataag gatgttgacc gtaacgtaga
      421 cttttacgcc aaataaatga gccgtccgga cagcctgtcc gatttcagca tcgtcgagat
      481 tatgagcaaa tttgcgggca ttgaagcctt tcccgccaaa ataaacggca tcggcaccgg
      541 cgtcaatggc cgtgagtacg ttttctaaag agcctgccgg ggctaataat tctgccatga
      601 aaatcctcct acttttaata gtttatagca tagagtttgt agtttgtaga aattgggttt
      661 taaatttttg ccctcactac aaactaaaaa ctaaaaactg cgaactcaaa aagcgctatc
      721 gtcatacggc aattacgctt ttacgattta tgtatcccag ccaactgctg ataaatacga
      781 gtgagcaact ctgccgtgac tgtgtagggt gtccgctgga cgccgccggt gggcagggtc
      841 atggtgacga atttttcgat ttcttcatca ctcaagtcga ccgtatcgcc catgaggtcc
      901 tgcatgaggg ccgtaaagtc atcgagtccg gccaggccca ggatggccca gaccttttct
      961 gccttatcgg ccatcgtttc ttccatgagc ttcatgtacg ccggcaagag caggccattg
     1021 gcccggccat ggctgagccc cttataatag gtcagcatat aacccatgct gtggaccatg
     1081 gtcgtccccg tctgggcaat ggtgatgcct gccagggtcg agccatagag caggtcttcc
     1141 cggacgacag gcgaaacgtc gcctttgagg tccgtcaaat ggacgccgag gaaacgcatg
     1201 gcttcttcgg cccagacatc gctcatgggc gtcgacgccg tggacatata gccttcgatg
     1261 gcatgggaca aggcatcaat ggccgtatca atggtgacgg cttcgggcac actgtccgta
     1321 aaggccgggt cggcaaaggc atagaccggg aaaatgagcg gcgtattgat ggactgcttg
     1381 gtcccggcct tgggattggt caggacgctg accttggtca cttcactgcc cgtcccggcc
     1441 gtcgtcggta cggcgacgat gggcaagggc ttgcggtacg ggccggtaaa caactgttca
     1501 tcatccagct cctgctcctg cgtgcagacc agggcgatgg ccttggccgc atccatgggc
     1561 gaaccgccgc cgataccgat gacgaaatcg acgccctttt ccaaggccaa cgcagcggcc
     1621 ttgcggatcg tcgctacaga cggattattt tcgacctcat caaaaaggaa ccaggaaatc
     1681 cccaattcat ccagcttttc cacaacagca gcctgggacc cattcacctt ggccgaatga
     1741 cgccccgtga ccagcagcgc cttccgtccc agatcagcca gcacttcgcc ggactgagcg
     1801 atacagccct ttccaaaaaa taccatcgta ggcatataat acgtaaacat gcagattccc
     1861 ccttcac
//
LOCUS       DAWCYS010000356         1864 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2932_length_1864_cov_5.129353, whole genome shotgun sequence.
ACCESSION   DAWCYS010000356 DAWCYS010000000
VERSION     DAWCYS010000356.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_1864_cov_5.129353"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          410 a          550 c          527 g          377 t
ORIGIN      
        1 cgcggaggac tactgtataa tattacgcct atgcattcgg cgacccagga cgggtggatc
       61 ggcgtcgccg tcggtacgcc cgggggcggg gccattgctt atacggaaga cgggaccaac
      121 gtctgctcgt cggaagcctt ttatccgctg ttgacacctc atccggactg ggctgaagaa
      181 agcccgctcc agatcctcga agccgtccag gaagtcatca gtaagacggc ggctcagctg
      241 cgttataaga acaaagaact ggcaggcatc gccttgagta cggtcatgca cagttttgcc
      301 ggcctcgatg aaggcagaga gcccctcatg gacatgcaga cctgggcaga cagccgcagt
      361 gcggccatcg tccgggagat gaagaaagac gaagccttgt gccgctcctt ctatggacgg
      421 acgggctgcc ccatccacgc ctgctatccc ctggccaaga tcatctggct gcgccagaat
      481 cagccggaac tcttccgcca gatgcgctat gtaggctcgc tgaaggatta tttattctat
      541 catttgactg gccaatgggt catcgataag tctgctgcca gcacgagcgg tatgtacaac
      601 gaacagactc tggattggga cgatgaaatc ctggcctatg ccggtatcac caaggaccag
      661 ctgccgcctg tcgtatcgac gacgtacagc caggggctct gtgaagaagc ggccaaagcc
      721 ctgcgcctgc ctgttggcct tcccgtcgtc atcggcgcta ctgacggcgt cctggtcaat
      781 gtcggcatcg gagccgtcga accgggccag ctcagcggga ccatcgggac cagcggcgcc
      841 ctgcgcatgc tgacccgcca gcctaagaca gacccgcaga tgcgcacgtg gtgctataac
      901 ctgaccgacg acatgtgggt cgccggcggg gccatcaaca acggcggcat gattctgcgc
      961 tgggtccgcg acaaaatctg ccattacggc ggcagtgccc tggaaacgct agacatcgac
     1021 ccttatgacc tgatgaccat gaaggcagaa catgtcgatg ccggcgccga cggcctcatc
     1081 tgcctgccgt atttcacggg tgaacgggct ccgtactgga attcggaact gcggggcatg
     1141 ttcttcggct tttctctaaa tcacagccgg tcccacatga tccgcgccgt catggaaggc
     1201 atctgttaca gcctgaacag cgtcatggca gctctcaagg aattcggtga cgtcaaggac
     1261 atccgcgtca gcggcagttt caccaagtcg aaactgtggc tccagatttt atcagacgtc
     1321 ctcaaccagc ccattaccct gccggacaac agcgaaggcg ctgccttcgg ggcggctgtg
     1381 ctgggcttca tttccagcgg caggttgaag agcatcgccg atacggccga cctggtccac
     1441 gccaagaagg tgtatacgcc tatggaagaa aacgttgccg tctatcagca gctctatgac
     1501 atcttcgtcc ggctctatca taacttgcag cgcgaatttg ccgacatcac ggcatatcag
     1561 cagaaattat aatttccagt gaaaaggagc gattgctatg gcacttcacg atattggcgt
     1621 cgtcggcatg gccgtcatgg gcagtaacct ggccctgaat atggccgacc acggctatga
     1681 cgtttcggtt tacaactata cgccggactt gacggaacag ttcctccaag aacggcctca
     1741 tgaaaagatt acgggctact tcgagctgaa agatttcctg gcttccctga agcggccgcg
     1801 caaggtcatg ctcatgatca tggccggcgc tcccgtggac agcaagctcg accaactgct
     1861 gcct
//
LOCUS       DAWCYS010000357         1861 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2944_length_1861_cov_4.764120, whole genome shotgun sequence.
ACCESSION   DAWCYS010000357 DAWCYS010000000
VERSION     DAWCYS010000357.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_1861_cov_4.764120"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          453 a          578 c          461 g          369 t
ORIGIN      
        1 tgttatgccg tcagccctga gtcgcgccgt caatacatcg aatacatcga tgaaaagtat
       61 aatgcggaaa ggctgaccca gatcctgcgc aacgtggcgg acatcatcgg cgcccacgtc
      121 ctcaacgtcg ccagccagga ttatgacccc cagggggcca gcgttaccat cctcatttcc
      181 gaagaagaag tgcacgatgc cgatgtcgtc tgtcacctcg acaagagcca tctgactgtc
      241 catacttatc ccgaaagcca tccccagaag ggcatcatga ctttccgggc cgatatcgac
      301 gtgtctacgt gcggccgtat ttcgccgctc aacgccttga attatctcct cgacagcttc
      361 gattcggaca tcgtcaccct cgattaccgc gtccgcggct tcacgcgcga tgtgaacgga
      421 aagaaactgt tcatcgacca ccgcatcaat tccattcaga attatatcgc cccggccacg
      481 cgcaaccgct acgatatgat tgacgtcaat gtctatcagg aaaacatctt ccataccaag
      541 atggtcctgc acgacttcga cctggataat tatctcttca atattaccaa ggatgacctg
      601 acgccgcgcc agcagcagca gatacgccgc caggtacgcc aggaaatgct ggaaatctac
      661 aacggccgca acatgccgtc catgcgctaa ggaggcgaga gcttggatac caagaaaaca
      721 ctgtgccgca tcggacagaa aatgaaagaa gccggactag tcgccgcctg tgatggcaat
      781 atcagtttcc gccgcgacga cggtaccgtc gtcattaccc cgtccggcgt gccaaaaggg
      841 gaattgaagc cgaagcacct gctcgtcgtc gccctcgatg gccatgtcct ggaagggacg
      901 ggaaaggcgt catccgaatc ggctctccat ttagaaattt atcggaagcg gaatgatgtc
      961 cgggccatca tccacgccca tcccgtcacg gccacggccc tgaccgtagc cggcataccc
     1021 ttccggccgg acatcgtcac cgaaggggca ttggtcctgg gcccggtacc gaccgtacct
     1081 tatgcaccgc cgggatcggc agaactggcc acggcctgtg ccgaagccgc agaaggagcc
     1141 gacgtcttcc tcatggaacg ccacggcgcc gccaccgtcg gccgagacct ggaacaggcc
     1201 ttctaccgcc tggaaaccct ggaaaccgta gccaagatgt accgcgattc cctcatcttc
     1261 gctgccacca gcagccaaca aaaaaataac cccgccgcca aagcccagcc cttgagctgg
     1321 tatttgaagc agtgaaaaaa aggagctgtc ccatgaagac agctcctttt tggcagtcag
     1381 cggctagcta ttaaaagacg tccctcttca caggatacgg caaaacatgg tatcattaaa
     1441 aggattgttt gtttcagtga gaattacgat aaggaggtat catcatgaat ctggatggta
     1501 tcacattaca ttgcattacc aacgaactgg ccgacatcct caagggcggc cagatttcta
     1561 aaatctatca gctcgacgcc cggtccctct atttccgcat cttcaacgat acgggcatcc
     1621 atcacctggt catcaccctg gacgactcgc cgcgcctgta cgtcgccgat accatgccgc
     1681 cgacgccgga tatcccgacg ggcctctgca tgttcctgcg caaatattat gaaaacggcc
     1741 gcatcgcgtc catcgcccag ctgcacctgg accggctcat cgaaatcgcc gtcgacgtcc
     1801 tcgacatgtc gggccgcctg gggagacgga aaatccatgt gtatctgatg ggctagtgcg
     1861 t
//
LOCUS       DAWCYS010000358         1854 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2954_length_1854_cov_4.188438, whole genome shotgun sequence.
ACCESSION   DAWCYS010000358 DAWCYS010000000
VERSION     DAWCYS010000358.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_1854_cov_4.188438"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          356 a          519 c          528 g          451 t
ORIGIN      
        1 cggcagggac tcctctaccg cctcgcgccg cttggccgcc agctcgaaac ggcgcgcatc
       61 ctgccagcgg gcgaggaaga gctggacggg cgtcttcatg ggcaggggct tcatattccc
      121 catttcgccg atgcgcccgg caatatattc ctcttcttca taggcttccg gcgtgtagac
      181 gtacaaagtc ccttcaaaat cggccgtaca gagctggccc acttcgatgg cttcccggag
      241 gatgtcgtgc agccccaggg catcagcctg gaggatgaaa gccgcccgcc ggaccagttc
      301 cgtatctggg atacagacgt ggccattctg ggtcatcgtc cgcaggacat aggtcaggcc
      361 ggcgctgagg cgctgcgggt cctgccggtc catgccatag gccagggcaa actggtcggc
      421 cgtcttgaag ccgatgccgt cgatttcagc gatcatgcgg tacggttctt ccgtcaggac
      481 gtagtggacg tcgtcgccgt atttctgcaa gagccggctg gcataacggc cggcgacgcc
      541 gtgctgttcc aggtcatagg cgatgtcgtt gacctgtttt tcttcataga aggattccgt
      601 aatgacagcc agcttcttag ggccgatgcc ttcgacttcc aggagccgct gcgggtcttt
      661 ttcctgtacg gccatggtat ccttgccaaa ggctgcgacg acgcggtggg ccagggccgg
      721 tcccatgccc ttgacggcgc cgctgcccag gaagcgttcg atgccgtcga ccgattcggg
      781 gatgatgcgg ctccagccgt cagcggcgaa ctggcggcca tagcgcttat gctggaccca
      841 gtggcccttc acttccaggc ggtcgccgac gaggggtttc gtcccgtggc cggtgacggt
      901 atacgtcgca tcatcgtctt catcgtgcag gcggaagacg cggtaatccg aatcgggcgc
      961 ttcaaataag aggcgttccg caatgcctcg tactacgatt tgttccatct tcacagcccc
     1021 atatgctccc gttcgtattt cttgctctca tccatgatgc actgataaat acgccgcaga
     1081 tacggcgaca attccttctg gacggtcaga ttggcgatct tatccaggac ctgccgttcc
     1141 cggcgggggt catagatttc catatgatgc tccttcttat aggcggcgac ttcagcgacg
     1201 acctgcatgc gccgttccag tattttcgtc aaatcccggt ccaatacgtc gatttcccga
     1261 cgacattctt ctaattccat acactagccc ttcccttctg cataatacct ttattataac
     1321 aaaaaaagcg ggttcaggcc attttattca tcttgtacac tctatacaaa atcggatttg
     1381 aaaattagta actctctcat cgcctgtgtg cgatttcgta ggggacgtcc tgaacaaggc
     1441 gtcccgctga gaatcctgtg aatagccaaa gtttatgacg atgccagaat gggctttgat
     1501 gaaatctcgc aacgggccaa aaaggcttgg gacagcccct acgcaacccc aatcggggtt
     1561 agttgattgt tttgtttctg tttatctttc gtctcagcta taattttttg catacagaaa
     1621 caggacctgt gcacagttat gtgcgcaggt cctgtttagg taatcccgat tcaattagtg
     1681 agctctggtc aacaaggcca cggactcgac gtgatgggtc cgcgggaaca tgtccacggg
     1741 ctgtactttg gtggtgtggt agccgtgctg gtcgaggtgg gccaggtcgc gggcctgggt
     1801 ggccgggttg caggagaggt agacgacgcg tttgggtttg ctctgggcga tggc
//
LOCUS       DAWCYS010000359         1849 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2962_length_1849_cov_5.488852, whole genome shotgun sequence.
ACCESSION   DAWCYS010000359 DAWCYS010000000
VERSION     DAWCYS010000359.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2962_length_1849_cov_5.488852"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          382 a          537 c          521 g          409 t
ORIGIN      
        1 cgtcgacgcc gcagcccaga tcatagatcc acgattcccc gacgagcggc accttgcaga
       61 tgcgcgtatc gaagccgatg tgctgttctt ccaaatactg catgacgcca gtcgaggcat
      121 tgaggccaaa ggcactgccg ccgctgagca ggatggcgtg gatgcccgac gcagccgcca
      181 taggactgag cagttccgat tcccggctgg ccgggccgcc gccgcggacg tcgacagcac
      241 agggcgcact ttccgggaac acgaggaccg tgcagcccgt gccgccctgg cggtcctggg
      301 cattgcccag gcggaaaccg ttcgtccggg ccatggctaa catatctatt tctttcatga
      361 agacacttcc ctttatttca tcttttcaaa gtctttccag agcatactgg gctggatgcc
      421 cgtattgttc tggtacttgc cgtgctgata cgtatcgtat tcgccgtcga tatcatgata
      481 gtatatctgg cagatttcca cgttcggata gatgcggatg ggctggacac agaagatttc
      541 cagcgtccag taaccggcaa aaccgacgtc gccgaagcca gccgtgacgt ggatgaagac
      601 gcccaggcgg ccgacggacg accggccttc gagcatgggg atatagccgt cagtccgggt
      661 atattcattg gtccggccca gatagagctt gttaggctgc aagacgtagc ccgacttggg
      721 gatataaatg gtcgatgccg gattgggctt ggccatatcc agttcttggt ggtcatagac
      781 catcagttca ttgtacaaag acaggttata gctattggga ttgacccgtt ccggatggaa
      841 aggttcaatg atgatttcct tacctaaatg cttgacaatt tccttgcctg ataaaatcat
      901 gagtgccact cactttctct agtaaattca cttcttatca tatagcaaaa ccggttcgat
      961 gtccacggcg cgatgagccg gtttttcttt ccctgtctca aagaagtcct ctacatagaa
     1021 cgtgaccttc tccccttcct ccgggcggac gcggtacagc gaagcggtac ggaaataata
     1081 ctgattgccg ccgtattccc ggaggaagcc ggccttgctg ccgggcagga ttttttcgat
     1141 ggtccccttg tggcgcgtct ggccggcgtg cttctcgtcc atccagaaac catggagccc
     1201 gtccatcagc tcctgccggt ccggttcggg aaaagcatag ctgtcggtct tggccaggag
     1261 ttcggccttg accttccaat gctggtccat gcggacccgt ttggccagga ggtaatggaa
     1321 ataggccatc ttctcataac cgcccatatt ctccaaagcc tgggcgaact ggaccatgaa
     1381 attgaccttg gccttgaact cgccgccagc caggaaggcc gacgccccat agcggcgcat
     1441 ggccgcgctg tcgccctcgg cctgggcggc aaagaacagg ccccggaaaa gaatccagtg
     1501 ctgcttgtac ttcagcaggt tctccaggcg ctgccgccct tccccgacgc ggccctggcg
     1561 cagcaggcag agggcgatgc ggtaagaaaa ccagatgtcg ttgtcatgat ggagctgagg
     1621 gaaatagttc agggcctgcc ggcacaaggt gatgcagtcg tagaacttct cttacttgac
     1681 caggatgcgg ctcatgaggg aataccagcg tgcccggtcc gaggcaatga cccgttcccg
     1741 ccccttcaaa gtcacggtct tctcctggtc cggcagctgg tccgggtcga ggcggcacaa
     1801 gtaggacccc atcagtcccg cctgaccggg ctcgttcttc aggccatcg
//
LOCUS       DAWCYS010000360         1812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2964_length_1846_cov_5.036293, whole genome shotgun sequence.
ACCESSION   DAWCYS010000360 DAWCYS010000000
VERSION     DAWCYS010000360.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_1846_cov_5.036293"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    821..830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          459 a          467 c          493 g          383 t
ORIGIN      
        1 tgcccaagcc tgtgcgccgt cagaagcctt catcggcagt catgaagata cactggctgc
       61 agaatcccgc cgtcatgtct taggtgaaat ggaaagggag cgtgaatgtg atgaacagcg
      121 taaccatgaa attgcaggct gatcctttga ttttacaggc cctgcaggaa gacatcaccg
      181 gtgaagacgt gacgaccaat gccattttgc cggaagactg ccagggtgaa gcggaactcc
      241 tgtgcaaaca ggacggcatc atcgccggcc tcgatgtctt tgcccggccc tttactctgc
      301 tggacgacaa ggtctggttc gagttcttgt tatatgatct cgataatgct cataaagtca
      361 agaaactgtc taaattcgtc ggcagtatgc gggccatctt gtcggccgaa cgggtaggcc
      421 tgaactattt gcagcgcata agcggcattg cgacctatac ccaccaggtc gcagccctgc
      481 tggaagggac ggggatgacc ctcgtcgata cgcgcaagac gacgccgaac atgcgggtct
      541 ttgaaaagta cgccgtcacc gtcggcggcg gcaagaatca tcgctacaac ttatcggacg
      601 gcgtcctcct caaggacaac cacatcagtg ccgccggcgg cgtggccaag gccattgcca
      661 tggccaaagc ctatgcgcct ttcgtccgca agatcgaagt cgaaacggaa acgctggaca
      721 tggtccgcga agccgtcgaa gccggtgcgg acatcatcat gctggataac atgagccacg
      781 acatgatgaa agaagctgta gccctcatcg acggccgggc nnnnnnnnnn atgttccggc
      841 aacgtaacca aagaaaatat cgccgccctg gccggtatcg gcgtcgatta catctccagc
      901 ggcgccctga cccattccgc gcccatcctg gatttatccc tgaaacacct gcacaggata
      961 tagtggttcg tggtccttga caagaggaaa atcagcgata taatgaagac aaagaaaaga
     1021 caacgatgtc gtccatgatg tcgttgtctt tttgcttttt attatatcgt attttgggag
     1081 ggggcctctt tatgtatgta tggcgaatgg aaaagatcat ggcagaaaat ttccgccaat
     1141 ggaaaatgct gcagcagtat gaaaagtatc agaaagggcg caaagccgtg cagcctaaga
     1201 aagaggacct gcatcttcgc gacggcgtcc attcggaaac gtgatgaaac ttgagagaac
     1261 gcgtgtttga caaaaagcct ctggatttct atactagtag ttgatagtgt agaaagaaag
     1321 aggcttattt atatggatat aaagaaagaa cattggagcc cgctgctcct cgactggttt
     1381 gccgcccatg gccgggatct gccctggcgc gacgaatcgc cgcgggatcc gtataaggtc
     1441 tgggtgtcgg aaatcatgct gcagcagacg aaagtcgaaa ccgtccgtcc ctattatgac
     1501 agctggatgg accatttccc gaccattccc gccctggcgg cggcttccca ggatgaagtc
     1561 ctgcggcagt ggcagggctt ggggtattac tcgcgggccc gcaacctcca tgaagcggtc
     1621 caggaagtac aggtcaaata cggcggccat gtgccggaaa ataaaaaaga cgtacagtcc
     1681 ctgaaaggcg ttggcgatta cacagccggc gccattttaa gcctggctta cggtcagaag
     1741 gaagccgctg tcgacggcaa cgtcctgcgc atctttgccc gcctttacga tctgtctatt
     1801 aaacacatca ca
//
LOCUS       DAWCYS010000361         1845 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2968_length_1845_cov_4.998324, whole genome shotgun sequence.
ACCESSION   DAWCYS010000361 DAWCYS010000000
VERSION     DAWCYS010000361.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_1845_cov_4.998324"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          320 a          553 c          586 g          386 t
ORIGIN      
        1 aaaagagggg cacatacgtg gcctggtcgt tgcgggcgtc gcggccgacg ggtttgccca
       61 gcaaagcttc atcgccgacg acatcgagga tatcgtcggt gatctggaag gtcaggccca
      121 tgtacgacgc ataggcttcc agggcctgcc gctgctgctg cgacgcatcg gcgacgatgg
      181 ctgccatgtc gatggccgcc ttgaagatga cgcccgtctt ggaacggtgc aggagttcca
      241 tgccttcccg gccgatggcc atgtcgccgt ccgaagccag gtcgaaggcc tggccgccga
      301 ccatgccggc cggtccggcc gctctggcga tgacggcgat gcagcggttc agtttgtccg
      361 gtgcaatgcc tttctggctg gccatgagct cgaaggcaaa ggtcagcagg ccgtcaccgg
      421 ccagggtagc caggccggca ccgtagacga cgtggttcgt cggcttgccc cggcgcaggt
      481 cgtcgtcatc catacagggc aggtcgtcgt ggatcaagga atacgtatgg atacattcca
      541 gagcgcaggc cagtcccgta tagggagagg catcctgttc cagggcttcg accgtcgcca
      601 gcagaaggac cgggcggatg cgcttgcctc cggccaggag gctgtaattc atggcttcat
      661 agagcctgga aaaacacggc tggtccgtcg tcaatacgtg ggacaggtaa tcgtcgacgc
      721 gcttacgctg tcctgccaga tagtcttgga aggtcataag tccaacaccc cttcagccgg
      781 cgcctcgtcg ctgctgtcct tatcggcccg gacatcggcc agggcctggg ccttctggcg
      841 ggcatattcc agggcgcctt cgcaggactg ggataatttg atggcctttt catataaatc
      901 gagggactgc ttcagcgtca ggtccggtgc gtccagttcc tgtacgagct gttccaattt
      961 ttcataattg ctttcaaaac gcttcaattt atcagtcgtt cttgccatat atgtccacct
     1021 ctttaacagc cgtcgtaatc gtcccgtcga cgacttgtat cgtcagcggg gcctctttcg
     1081 acacgctgtg gatatctgta aggatcttgt catgctgcat gagctggccg tagccgcggc
     1141 gcacgagggc tgccgggttc agggcctgca gggaagccgt cagggtattc agcttcccct
     1201 gcttctgttc cagatggagg cgcagctggc ggtcggcctg ttccatcagg cctgccaggc
     1261 gtgcttcgcg catggccagg aaggcatcgt agcgctgcag gttcagccgg cccgccgcct
     1321 gttcgaccca gcgctgccgg tcttcgatgc aggccatgac ccgttcgtaa gcccggtccg
     1381 ccagtgcggc caggtccatt tccgcttcgc tgacgtcaaa gaaagccatc tcggccgcgt
     1441 gggtcggcgt cgccgcccgc acgtcagcgg cataatcggc cagggtcgta tccgtttcat
     1501 ggccgatggc cgtaatgacc ggtacgctgg catggtaaat ggccttgatc acagccgggc
     1561 tgttgaagca ccacaggtct tccatggagc cgccgccgcg gccgacgatg aggacgtcca
     1621 ggtccttgcg ccggcccatc tcgccgatgg ccttggcgat ggccgtatcg acgccgtcgc
     1681 cctggacggg gatgggatgc aggatgattt cggcataagg attgcgctcg ccggcaatct
     1741 tatagatgtc gtggagcacg gcgccggtct tcgacgtcag gaaggcgatg cgccagtgga
     1801 agcgcggctg ctccttcttc aggctgctga cgaaatagcc ctgag
//
LOCUS       DAWCYS010000362         1834 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_2993_length_1834_cov_5.163013, whole genome shotgun sequence.
ACCESSION   DAWCYS010000362 DAWCYS010000000
VERSION     DAWCYS010000362.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_1834_cov_5.163013"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          463 a          523 c          507 g          341 t
ORIGIN      
        1 tcctggccgt cgtcgccgcc atcgccttcg tccttgcatg gcggtgcctg tacctggatt
       61 accagtcctt ctaagagggg ccgatccagc aggatgtgcc ggacacgagc cagacccaga
      121 gcgacagctc cgtctttgcc cagaccagcc cggacaagcc gctctacgtc ctgctcctcg
      181 gcaccgatgg cggggacccg cagcaggcga atttcgtcgg cgtcgcggct atcaacaagg
      241 ccaagaagca catcgacatc atcatgctgc ctgacgatac gaaaatcgaa gggcgcaagg
      301 aaaagggcat ccaggaattg caggatgtct acagtgaagg cggcatgagc ctggtccggg
      361 ccgtcgtcga agacattttc cacatcccca tcccgtatta tgcgaccttt acggccgatt
      421 ccttctcgaa gatggtcgac ctcagcggcg gtgtgccgat ttacgtcgaa aagaacatgt
      481 accacggcga cgaagcgacg gggaagaccg acatcaacct cttccagggc tatcagaccc
      541 tgaccgggaa ggaagccgcc ggctatatgc gctatatcga cgcggacggc tatctgtcgc
      601 ggacgcagcg gcaggaacgg ttcatcaaga acttctatga agaccggcag gactgcttcg
      661 gcgtgaccaa tgccttcttc atgtaccggg cctggcacaa cgtcgattcc aacatttccg
      721 ccaaggatat ggcccagctg gccttcgatt tccgcggcgt gacggctgac aacatcgatt
      781 tctatatcct gcccggcgaa atggccatga gcggcaagaa cggcgatacg aaatactact
      841 ggacctacga tccggtcgaa gtccagaaag tcattggccg cacgaacaac gccatctcga
      901 cggcgccgga accggatgat acgaaacaat aattacgtca taaaggagtt actatataca
      961 tgccaattac catgaaagca gcgaaagctg aacaaccgaa agaactgaag agctatgaaa
     1021 ttgcctgcaa ggccgctgac gacaagaaag cctgggacat cgtcaccctc gatatgagcg
     1081 attcgtccct catggcggac tacttcgtca tctgcagtgc ccgcaacaac cggcagtccc
     1141 agagtattgc cgacaatatc gaagaacaga tggaaaagaa cggctatgat gtccgtcacg
     1201 tcgaaggcta ccgcgaaggc aagtggatcc tcatcgatgc cggtgaagtc attgcccacg
     1261 tcttcgtcga acaggatcgt cagtattacg atctggaaaa cttatggtgc gacgcaccgc
     1321 gcattgccta cgaaggtgaa taagatgacg acacttcagg aaaatagtat tgttacgctg
     1381 gaagccctgc gccagagcga tatgggcgtc tttttgaacg gcggtacggg caatacgaac
     1441 gacgatattt tactccataa gaaccagcag acaggcgacg tcaaagtcgg cgacaaagtg
     1501 acggtcttct tgtaccacga cccgtcaggc cgcctgacgg cctctatgcg cctgccgaag
     1561 atcaaagtcg gccagatcgg ctatgcgccg gtcatcaata cgacgcgctt cggtgccttc
     1621 gtcgacgtcg gcacggaacg gggcatcttc atgccctttg ccgaaatgaa aggccgcccc
     1681 aaggaagggg aatacgtctg ggtcaagctc tatgaagaca agtcccaccg tctggccgtc
     1741 tctatggaag tggaagacga aatgcgccgg gctacgagcg cggcgacgga cgccaaagtc
     1801 ggcgacaggg tcgaaggcgc tgtctacaac ctga
//
LOCUS       DAWCYS010000363         1828 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3006_length_1828_cov_4.968415, whole genome shotgun sequence.
ACCESSION   DAWCYS010000363 DAWCYS010000000
VERSION     DAWCYS010000363.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3006_length_1828_cov_4.968415"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          334 a          569 c          536 g          389 t
ORIGIN      
        1 acgagcgacc ctcgggccat cggcggcggc caggatcagc acgatgtcaa ccagaaagcc
       61 ctgtcggccg ccctggacca ggtcgtcacg tctgtcgtca actacgtcgg cgtcgacctc
      121 aacacggcat cgccggccct cttgcagcac atcgccggcc tgacggcggc gacggcaggc
      181 aatatcgtcg cctaccgcaa cgagaacggc cccttccaga accggcagga cctgctcaac
      241 gtcccccgcc tgggcccggc gacgttcacc cagtgcgccg gtttcctgcg catcaaggac
      301 gggacggagc ccctggacaa cacgtcggtc catcccgaat catacggcct ggccgaaaag
      361 atagccggcc actacggcct gagccatgac gagctcaagg acccggaaaa attagccatc
      421 ctgcgcgata agatccagat gaacgccgct cccaaactgg ccgcctctct cggcgccggt
      481 gagccgacga tcaaagacat cctggaagaa ctgcgcaagc ccggccgcga cgtccgcagc
      541 gacttcccga aaccgctgac gcgccgccac gtcctgtccc tgtcagacct gcagattggc
      601 tccgtcgtcc gcggcacggt ccagaacgtc gtcgacttcg gcgccttcgt cgactttggc
      661 ctcaagacgc cgggactggt ccaccgctcc cagctgagcc ggcatccctt ccgccatccg
      721 acggacgtcg tccacgccgg cgacatcgtc gaagccgaaa tcatcagcgt cgacgccgcc
      781 cgcggccgca tcggcctgtc catgaaaaaa gtgaagaaat gagtgtgaaa aaggagctgt
      841 cgcaatgcga cagctccttt ttaggggtta gcagatggct ttaaatttaa agcttttctg
      901 taggggcgac cacgcggggc gcccgccccg ggatatgggt gattcaccat tcagccgtag
      961 gggccacgaa cattacaaac tattccattc gtcgttgagt ttatcgtagc ggttcgatgc
     1021 ggcctggccc cgctggaagc ctgggcggta gtcctgtccg cgctggctgg cgctgacgcc
     1081 gtcgtagagg ccgcggacgc ggtcctgttc ggcctggaag agggcatcga gcttatattt
     1141 catggccgta ttgtcgatgg acgtatcgtt gtccagctgg ctgcgggtct tggaaatctg
     1201 gacgtagagc gtattggccc ggctggtgaa ttcgcgattg cccttgaagt ccgtgtggtc
     1261 attcaagtag gcgttgatgt cgccggccaa tttgccgata tcgtcgttga acaagtcctt
     1321 gctgctggca taggacgcca ggatctgctt atccggattg gccttggctt caggctgcgg
     1381 cttttcatcg ggcttggtct ggacgctgac gtatttttcc aggtagctga ccaggtcgtc
     1441 gccatcgacg gcagctgtga aatcggcacc ggggaccttg atggtccgcg ccgtcttctt
     1501 ggccttctgg acttcgtcgc gcaggtcatc caatttttcg ctgatagcgt cgtctgacgg
     1561 attttcagca atcgtcatgg ccgtgtcata catggccatc tggtctcgca tgacttcgcg
     1621 gatagccttg tcatcggcgg catactggtc gggaatggtc ttgggctgat aattcttggc
     1681 catcttttcg acgttcttgc ggctcttgcc caaatccttg accagggaat cgacatcgtc
     1741 gcctttgctc ttctggctca ggcgcttggc cgtactggtg gcagattcat tttccgacgc
     1801 cagctgctgc gacagggaaa cggcatcc
//
LOCUS       DAWCYS010000364         1820 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3019_length_1820_cov_4.534844, whole genome shotgun sequence.
ACCESSION   DAWCYS010000364 DAWCYS010000000
VERSION     DAWCYS010000364.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3019_length_1820_cov_4.534844"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          491 a          434 c          503 g          392 t
ORIGIN      
        1 ctcaggcggg ggcctacgac tgtgatctgc tcgaactttt gcacgataca gcctatggag
       61 aatgtggggc ggaagactgg ctcaacgacg tcacgcacgc cgacatgcga gatctgggcg
      121 tattcttaga caacgccttt atgtgctggc ttgacaagca cccggaatac aaaccaggat
      181 tttatacatt gaccgattta cagttattcg acaaagaagg tgagccgctt gattaatgga
      241 gccacagcca aactgatttt caatattcgc atcgcgtact gtgaacagcg ggatggaatc
      301 gaacttgcct gcgatgattg cgccataaag agccgttgcc gcgcgatgcg gcacgaccac
      361 agcgcgccga cggagctgga cccatatccg gacaaggaaa caaaaggggc caacctggaa
      421 gacccattcg atgattaaag gaggaaaaga acatgatcga taacagcagg gaaacgattt
      481 ccggagccgt agctgtaaaa gccttgaaca cgattataat ttattgtgcc acgcacctct
      541 gcccggattg ccctttaaga caggacttag aaaaagataa ttcctgccct atcccgaaac
      601 tggatgtgaa aaagtatgtc ggctttttca aggacaccgt cggtaagcgt gctcctgagc
      661 caccgaagtt cgatggcagc cttcagctca gcccggaatt caagagctac ctgacaagtg
      721 tgctcgaccg ggcgctggcc gatgaaggca ttgacagcca ggacctggtg aaacacggag
      781 aacttgccgt gaggatcaag gccaacggcc tcgtaaaggt cggggcccag cgggatggcc
      841 aaatcaaaat actgggcacg gcgaaatgcc acccgaacga tgctttcaac ttagagacgg
      901 gcgtacgatt agccgtacac cggatggtac aggacgcgga taagccgttc gagcaagtgg
      961 ccggttacgt atattacttc gtcgactata acggtggcat catgggcaag ccctggaacg
     1021 atgacgtgct agacagctgc aacctggccc tggataactg cttcgcctcg acgtttgcgg
     1081 cctggctcca cattacgccc gtaaagcggg gcatccagca cctcgccgat gccctgcggc
     1141 tgacgctccc ggagccggag aaagacggtg attagatgcg acagataacg tggattgtac
     1201 tcatagctta cttcatgatt tgcatcaccg cctttctgac gctgctagtc atgattttca
     1261 agtaaaggag gggatacgca atgattgaca agacgaaagc cgcggcctac cgggtactct
     1321 tcatcgggag cctgctcttc tgggcggtac tgattggcgt cctggtagcg gacgcgtggt
     1381 aaggagggct aatggagaaa ttagggtttt gcttaggctg gatgttagcg tgtatattca
     1441 cgctgctcat cacagaaatt gaatacggct gccataataa gtccaccttt tatatagtcg
     1501 cgatgctgac tggcggcata gcagccgcac tgctattcac accgtggtaa ggggacattt
     1561 atgatgatcg atgaaaataa agcgctcata gcagggctta aaaaaatcaa gaaagattgt
     1621 atggctctcg cagattgcga cgaatgcctg ctttatggtg aagacggctg tatgctgaac
     1681 atctacgaat taggcggccg cccttatgtt tgggatttaa gcgtactaaa aggaaaggag
     1741 gaatcgagat cgagccacaa tttatctgtg aaaaatgcct agaggcgtat gatacagccg
     1801 aggaagctaa agaatgagaa
//
LOCUS       DAWCYS010000365         1797 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3053_length_1797_cov_4.361653, whole genome shotgun sequence.
ACCESSION   DAWCYS010000365 DAWCYS010000000
VERSION     DAWCYS010000365.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_1797_cov_4.361653"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          289 a          540 c          582 g          386 t
ORIGIN      
        1 ctggggattc aggcgggacg ccctgtgggc ggcccctacg agccacgagc cacgactcac
       61 aaagtgccgt gcagggcttt gagcagggtt tcgcgtttgt cataggccct gcggtaggta
      121 gccgtatggt tttcgtcggg cacgtaggtg gccttgattt ccagctggcg ctggagggcc
      181 tgtttgaggt ccggtatgtc gcctgcggca taggcggcga cgatgcagtt ggtcgggacg
      241 gcgccgccgg cgactttgag ggtcacgatt ttcgtatcca gcatgtcggc cttgatctgg
      301 ttccacagtt catcgcggct gccgccttcg gtgatggtca gctgggtcag gtcgatgccg
      361 gccttgcggt agcggtcggt gatttccata tagtcatagc cgatgccttc gaggacgcag
      421 cgccacatgg ccccctggtc ggagtcgagg gtcaggcccg tgaaggtccc gcggacgttg
      481 ggatagtcgc cgttgccgcc ggtcaggtag ggaatgaaca tggccccgtc gcagccgggc
      541 tgatggcgcc gggccagttc gtccagggtc ccgtagtagt catcgccttc ttcgcgggca
      601 atgctgtcct tgaaccagcg cagggccagg ccgcctgtgc ggaccatgcc ccagtagaaa
      661 tacgtccccg gcagggtgcc ggaattgaag atgaggttgc tccccggcac ggacagttcc
      721 ggaatgatgc cgtccgtggc ggcgcagaac atggcgcacg tcccggcgac gtcgacggcg
      781 cggcccgctt ccaggatgcc gctgcccatc atggattcca tcgtgtcgcc ggcgccggcg
      841 cagatgggga tacctgcagg gaagcccgtt tccatggcat cttcttcgca gagcctgccg
      901 atgatgtccc agggcttgac gatgcgcggc atatagcgtt cagggatgcc caggatatcc
      961 agctgttctg gcgaccattt cttctggacc acgtcatagc ccaggcccca gccggacatg
     1021 gcgccccagt cgatgaaggc gtcttcccct ttgaggccgg ccaggtgcat gaggacgtac
     1081 gggcagttgt ggacgaactt gacgcccttt tcctgccagg ccgggacgtt cttcatgaac
     1141 cagcgagcga acatggccgg gaacatgatg ttcggttcgg cattgcccgt ttctttgccc
     1201 cagatggcca gcttctgcgc cttcagggct tccacgtcgt cggccgtgcg gctgtcgaga
     1261 tagttgacat aaggcgtgat ggcgttgccg tcggcgtcga taccggcgat gccgcagatg
     1321 atcccgtcgc ccatgatggc ccggacgttc ttcacgtccc agcccttctc ttccatctgg
     1381 cggctgcacg aagccatgca ctgcttgatg aggcggtaat attcatcgac gtccatctgg
     1441 acccagcccg gctgggggta ggccagatgg gtcttggccg tgtccatagc caggcagtgc
     1501 tggtcttcgt cgtagacggc gactttgacg ctctgcgtcc cggcatcgaa accgaggtaa
     1561 tacttgtcac tcataggtaa cacatccttt ccgttcagcc ccggcaatcc tttaccatcc
     1621 cgccaaggcg gctcacgagg cccgtcgtcg actcgttcgg tttcgggaag cggcgcaggg
     1681 cggtcatggc ttcgacgtaa cagaagaact cttccatggc cgtctcgatc tgccccagcg
     1741 tctggccgtc gatgtgctcg atgagggcca ggtcgtgctg ttcatagcgg ttgatat
//
LOCUS       DAWCYS010000366         1784 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3081_length_1784_cov_4.714286, whole genome shotgun sequence.
ACCESSION   DAWCYS010000366 DAWCYS010000000
VERSION     DAWCYS010000366.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3081_length_1784_cov_4.714286"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          534 a          408 c          452 g          390 t
ORIGIN      
        1 accgggagga gcatctcgac agcaatcaac ttcttcatca agaaatccct cgcggaacaa
       61 cggagcccct tcgagattaa tgataaacgc gatagcgaaa agtcgtaaaa taaaacaggg
      121 tcttgtcgca tgtgtgacaa gaccctgtaa gtggctagca gatgacttta aatttaaagg
      181 ctttctttgc aaactacaaa ccccgtacta taaagaaagg gaccacgcac gatggtagaa
      241 aacttcgtgc gtggtcccct tttttgtccg cttttcctcg gattcagatg cggcttcgcc
      301 gcgacagact catgaaataa acgcagtttg ctggtcgcag accgcaaaaa taaccccaat
      361 cggggcattc gtaggggacg acctttaacc tctcagaccg cctttggcgg ccagctcccc
      421 tataaaggga gccttgtgcg gcccgccaga gagatttcat cacagcccat ccgagtataa
      481 tggccggctt ttacgagcca cgagcatctg tcatattata caataaaagt gaatctggag
      541 gtgtgacgtc atgtccgatt cggcacaaca ggccaaagcg cagaaaaaag cgtatgacga
      601 ggcgtgcaag agggttcttt cagagcgggg cattatggct catatcctga aggcctgcac
      661 ggaagaattc aaggattgtg atttgcagga tattgcccaa cgctatatcc agggcgaacc
      721 gagcatcagc cgtattgcgg tggaaccgga agctatttca ccgcgcatcg aaagcgagca
      781 gacggaagac aaatccggaa cggaaggaac ggtctattac gatatccggt ttcatgctgt
      841 agctcccatt gatggaaagc gcatccagct catcatcaac ctggaagcgc agaacgattt
      901 caatccgggt tatcccttgt taaagagggc cgtctactat tgcggccgca tgatttccag
      961 tcagtatggt acggttttcg tgaaatccca ttacgaaaaa atccagaagg tctattccat
     1021 ctggatctgt acgatgccga cgaaaaaatg ggagtacaat atttccagtt accaattgat
     1081 tgagaaacat ctaatcggtc atacacaggc cgagtggagc cattacgatt taatcaatat
     1141 cgtcctggtc tgtctgggaa gcaagagcca taaacagcta aaaggcatcc tacggctgtt
     1201 gaacatgctg ctcttggata acatcagctc tcaagaaaaa cagaaactat tggagaaaga
     1261 atttgacgta acaatgacac cgcacttagc gaaaggagtg gcagccatct ctaacttgag
     1321 cgaagctatc gaacggcgtg tagaattacg tggccgtaaa ctaggtgaag aagtgggaga
     1381 aaaacgtgga gaactacgcg gcaggaaaat tggagaaaaa attggacaag aacgcggaga
     1441 aaaaacggga agaaaagcgc tcagtgtatt gctccagaaa ctgatgcaag aaggcagaaa
     1501 agaagacgtc gaccgcgtct tgcaggacga tgaataccag gaacaattgc tgcgggaata
     1561 tcatttgaaa taagagggta atcatttatg acggcgttgt catataagac agcgccgttt
     1621 tgcgttcttc ggattcagat gcggctatgc cggaaataaa cgaagttcgc tggttgcagt
     1681 tagcagaccg caaaaatagc cccaatcggg gcattcgtag gggccgactt ttaacctctc
     1741 agaccgcctt tggcggccag ctcccctata aagggagcct tgtg
//
LOCUS       DAWCYS010000367         1781 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3084_length_1781_cov_6.198725, whole genome shotgun sequence.
ACCESSION   DAWCYS010000367 DAWCYS010000000
VERSION     DAWCYS010000367.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_1781_cov_6.198725"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          421 c          498 g          435 t
ORIGIN      
        1 aaagggcatt atccgggaaa tgctgcgcca tgtgacggct gaaacgtaga cggatttact
       61 ttctctttta caggggcgcc atgtgacggc gttgatacgg gaaggcgtca tttatgaaga
      121 tattggccgg gatacggatg ccctctatac gatgctgctg acgacgggct atttgacggc
      181 tgtcagccgc aagcggggaa tcggcggcat acgctgtgaa ttggtcattc ccaaccggga
      241 agtacaggat gtatatcgtt ttgaaatcct tgacaagatg agaggtcgat tttccgtgtc
      301 ccggctggaa accatgttcg atgatttact ttcaggcaac ggaaaggcct tttcggaact
      361 gctgggaggc tatctccggg acctggtcag cgtatatgat acggccaata aagaaagttt
      421 ctatcacggc ttcgtccttg gcatgacggc gttatttgta tccgattata ttatcgaatc
      481 gaaccgggaa agcgggtacg gccgcttcga cctggcaatt attcccaagg atgtgtctaa
      541 ggccggcgtc atcatggaat tcaaagtggc ttcgagggaa gctgacatgg cggcgaaggc
      601 caaagaggcg ttgcagcaga ttgaagaaag agaatacgtg acggaattcc gccagcgggg
      661 aattcagcgt gtatggaaat acggtatcgc cttttgcggg aaaaaaatcc ttgcatttca
      721 aggaccaatg tgctaaacta ttctagcata ggttttgaat gattgataca gaaagaggtg
      781 taagtggtga aagaaggtat tcatccgaag tactacccga atgcgaaagt tacttgcgca
      841 tgcggcaata cgtttgaaac aggttctacg aaaccggaac tgcgtgttga aatttgctcc
      901 aaatgccatc cgttctttac ggggcagcag cgcgctcagc aggctcgcgg ccgtatcgaa
      961 cagttcaata aacgttatgg taaaacagct aaataagcag cagtacaagt gggcagcaga
     1021 gcagaaatgc tctgctctat ttgtatgtac agaaatggag gcaactcttt gaagaaaccg
     1081 aagacgtacg tcggcggcca ggccgtcatc gaaggggtta tgatgcgcgg tcctgtcagc
     1141 attgccacag cagtgcggac gccgtccggt gaaattactg taaagaaaga cgccgttcac
     1201 tccattaccg aacggtatcc gattttgaag aagccttttt tgcggggcag tgttgccttg
     1261 tgcgagtccc tggtatacgg catgaagagc ctgtcctatt cggcccaggc tgccggcgaa
     1321 gaagatgagc agctgtcgaa cacgcagatg gccctgacta tggccgtgtc tgtcgtcctg
     1381 gccatcgtct ttttcctggc catcccgacg tatgcagcca agtttattcc cggcgtggct
     1441 gacagtgcct ttcgcctgaa cgtcgtcgaa ggggtcctgc gcctcgctat atttctatta
     1501 tatatatggg ggatttccct gacgtcggac atccgccgcg tctttgaata ccatggtgcc
     1561 gaacacaaga ccatttggac gtatgaatcg ggagaagagc tgaccgtcga gaacgtcaag
     1621 cgccacagcc gcctccatcc gcggtgcggt acgaatttcc tgctcatcgt catggtcgtg
     1681 tcgattttcg tctttgcctt tttgggctgg ccgaatctcg tcgaacgcat cgcatcccgc
     1741 gtgctgctca tgcccgtcgt cgccggcatc agttatgaaa t
//
LOCUS       DAWCYS010000368         1765 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3110_length_1765_cov_10.997076, whole genome shotgun sequence.
ACCESSION   DAWCYS010000368 DAWCYS010000000
VERSION     DAWCYS010000368.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3110_length_1765_cov_10.997076"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          406 a          476 c          499 g          384 t
ORIGIN      
        1 tgagactctt cacttcgatg tctttcgtgc tttttttgtt aatcattttg ggagcagcga
       61 tgattagacc tgtcaattcg tcggcagcat agagggcttt ttccatcaag tgctccggtg
      121 cgacatcgac acagatgcca tagccatggc agacgacggc atggataaag gcatcgtcgg
      181 cgtcgatttc agccagtaat tccggtgcct ttttacagtg ctgatccgga tatttttcaa
      241 aatccacatc gtggagcagg ccgacgagtc cccagaaatc gacgtcatcg ccatagccca
      301 gttcctgggc ataccagcgc atgacgcctt cgacggtcag ggcatgcatg atgtgaaaag
      361 gctcctgatt atatttctgc aataaagcca atgcttgtga gcgttccatg tctttatctc
      421 cccctagcaa atctcgattt accccattat agcataaaaa acgggcctga ctagtctttt
      481 ttcacgaaag cgcctgtatc ggtcatcaac gaggccaggc ggttcatgcg gaagtcttcg
      541 acgacctgga tgaaaggccg gtaatggatg ggattttcgg caaacccgaa gagatgggac
      601 gggcagtcgc aatcggtaca gccaaagccc tcgatatgcc agtctgccac gacatcgacg
      661 gcccgggcca ggtcgtgctc caggtcatcc gccgtccgga cgggcagggc ggcgacgacg
      721 tcggcttcct ggcgcaggta gtcgatgtgc cagtgcatct tcttgcgctt gcggctgtga
      781 cgggccagac gggcagccag gttcttccgg gccgacccgg tatagacgta atagccggca
      841 ggaaaaggga actccccttt cgaaccgatg gcgatatcct tcgcatctgc caggtgaagg
      901 atgaataaat aatcaccgct gtcatggttt tcccgttcca ggatggcctg gggataggtc
      961 aggacgccgg ctacgccggg ccttgaaaac gagccatccc agtgcagggc tagggccttc
     1021 cagtccaagt gcggcgccgc ttcataaaag gcctgggcaa aggccggatc cgtatggtag
     1081 tccggcagga accagcgggc ccgattccag tggaccagga acaagacgcc cgtgtggatg
     1141 ccctggcggc tcatggcagc taattccaag atatgtttgc ggccccgttc ggtgacggca
     1201 tcgggaaaca tggcgccgtc gcggctaaag agggtacacg atttgacttc gacgaaaaag
     1261 cgctcttcgc cccggcccag gagcaggtcg aaacggctgt cgccgacggt gatttcccgg
     1321 cggatgaggt cccagtcttc ccagcccggg atgaggtgtt cctggaccag ccaggctgcc
     1381 gtatcattat tgtactgtgt atcgagcagg ataggcacgc catcgcgctc gataccgacg
     1441 acatcgtaag ccgtccggcg cttgggattg tctgccggcc ggacatagac cgtcacaccg
     1501 ggaaagagca gttcccacat gcgcccggga ttgggcatat gacaagtcac gacatcgcca
     1561 tcgagagaaa catgggcgat gaagcggttc ggcctgtcga caaaggcccc tttgcgaata
     1621 gaaaacattt ccatatgata ctcctgtctg tgaaaaagag gggctgtaac ataatagcct
     1681 catcgaaaaa aatggactca gttatctgaa cccatttttt taagcaaaag aggccccaaa
     1741 gggcctcttt gctggtataa ttatt
//
LOCUS       DAWCYS010000369         1759 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3117_length_1759_cov_8.917254, whole genome shotgun sequence.
ACCESSION   DAWCYS010000369 DAWCYS010000000
VERSION     DAWCYS010000369.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_1759_cov_8.917254"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          425 c          379 g          437 t
ORIGIN      
        1 tactgccact cttaaaattt tagaacgtgg tcttgacaag gggcccatta tactccatcc
       61 gtcaaagacg gctcaatttt tacggatccg gaaaagcata aagaattcgc ttttgctaag
      121 acatatggca gccgtctgta tgagctattc aatgccgaag aatcagctgt caccatgttc
      181 aaccactatg gcaaagcacc tatttatacg atgaaaattg cctatggaaa taatccgaag
      241 ctcgtcggtg aaaaaacggc ctttacgtcc ggctctatcc acggcatctg gattcctttt
      301 gctaccggca tcccaacagc tacgacagca ggctatccgg caggttcttt cgacaatccc
      361 ggcgcgctgg cactgacggc acaaatacag gattatctgg gtaatttcat gcgtacaggg
      421 aatcctaatg gcaagggcct tgtaacatgg aagaaatgga ccagcgcaac aaagggaccg
      481 agccagttat atgtcaattc cgatgggaca aaagtaaata tccatcaaga atacggccgc
      541 acgacctatc agtctatcct tgacaaaata gtcagggata cgtctataac cgagaatgca
      601 aaagaagaac tcatcaagca cgttctcagt ggccgctggt tcagcaaagg acttgacaca
      661 ttcttccata acaattaaga aatcatcact tacatacaat caaaaaatcc tgcactcata
      721 aaccattgga attgtgagtg cagaattttt aatagactta gtgcagactt ggataaactg
      781 gcttcatcaa tttccaaggc aacgtaaaaa gtttaaattt catatttttg catatagaac
      841 aaggactgta caagattacg tacgacaagc ccctttttct ccaaatatac aaatctagcg
      901 ggttttgcta caatagaatc aatctgtaat tctaactcaa tcgactcttc cctattattg
      961 gatgtaccat tcttattttc aggaggatca gcatggatta tttaaatgat atgttttctt
     1021 tgaaaggcaa tgtcgccctt gttacgggcg gttcttacgg catcgggttt gccatcgcca
     1081 aagccttagc ccgcgccggc gccgtcatcg ccttcaactg ccgcagccag gaacacttgg
     1141 accaggctat gaaggattat aaaagagagg gcatcgacgc acacggctat atttgcgatg
     1201 tcactgatga aattcatgtg cagcaattag tcagcgaaat agaaaagacg ttaggaacca
     1261 tcgacattct cgtcaacaac gccggtatca tcaaacgcat tcccatgtgc gacatgacga
     1321 cggaagagtt tcgccaagtc gtcgatatcg acttagtcgg cccgtttatc gtatccaaag
     1381 cggtcatccc cggtatgata aaaaaaggcc acggcaaaat catcaatatc tgctctatga
     1441 tgagcgaatt aggccgtgaa accgtatcgg cttacgctgc agctaagggc ggactgaaaa
     1501 tgctgacccg caatatctgc tctgaattcg gaggagcgaa tatccagtgt aatggcattg
     1561 gtcctggcta catcgcaacg cctcagacag ctccgctgcg cgaaaaacag cctgatggct
     1621 cccgtcatcc ctttgaccag ttcattgtct ccaagacgcc ggctggtcgc tggggcacaa
     1681 cagaagacct cgaagggccg gctgtggtag tgtcaagatt ctttcgcaaa ttcatttcat
     1741 ccgccagtaa atatcgtaa
//
LOCUS       DAWCYS010000370         1750 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3129_length_1750_cov_9.522124, whole genome shotgun sequence.
ACCESSION   DAWCYS010000370 DAWCYS010000000
VERSION     DAWCYS010000370.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3129_length_1750_cov_9.522124"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          327 a          459 c          513 g          451 t
ORIGIN      
        1 atcccctaac ccctaaccac taaccactag ccactaacca ctagccactt aaaaagggct
       61 tgtcgaatga cgacaagccc tttcttcacg cctcaatcac tttccccggg ttgaggatgt
      121 ctttcgggtc catggcccgc ttgatggtct tcatgatttt cagttcgcct tttggcgtga
      181 actgttccat atatttcagc ttcttggcac cgatgccatg ttcaccggcc aggcggccgc
      241 cgacgctgta ggcataggcg tagacctgct ggtggaattc ttcgacgttc ttttcccatt
      301 cttcgtcgct catatccatc ttgcagagga cgatgtgcag gttgccgtcg ccgatatggg
      361 cgttgatctt gacttcaaaa ggatagttct taccgatgtc catgattttt tcaatggtcc
      421 cggcgatttt atgtaccgga acgacgacgt cgtccgtgag gaagaccttg ctctggagtt
      481 cgacggacgt ctggcagtta cggcgcatat tccagacgcg gtcgtcagct tccaggactt
      541 cggcagcgcc cgattcttca cagatcgtgc agacagcgtc catcttggtg tccaattcgt
      601 cttcgtggaa cgtttcgacg gtgatgatga cgtaaatgcc gtcgtcgtag tgcggcatgt
      661 ccttgtaatg gcagtagtca gcgcagccgc ggacgtagga attgtccatg tattcgacgc
      721 tggtcgggtc gatgccggct ttcatcagct tcggcaccat gtgcagggcc tggcgcgggt
      781 ccgtgtagat ggccaggacg tcgaagcggt acggcggcag ggggatgagc ttgactgtga
      841 ctttggtgat gatgcccagc gtcccttcac tgccgatgat gagctgttcc aggtcgtagc
      901 cggtagagca tttcttgagg atgcggccgc attcgacgat atcccctgta ggcgtgacga
      961 tttccaggca gtagacttgg tcgcgggtga cgccatagcg gacggcgcgc aggccgccgg
     1021 cattggtggc caggttgccg ccgatgaggc agctttcggc gctggacggg tcgccggcat
     1081 acatgagacc aacttttttg gcggcgttct gcaagtctac cgtgcggacg ccggcttcga
     1141 cggtcatgta catgccttct tcattcagtt cgatgatctt gttgagacgg tccatgcaga
     1201 ggaccaggcc gccgcagaca gggatggcac cgtcggccag gctggtgccg ccgctgcgga
     1261 cggtaatggg gaagtcgtat tcattggcca atttgacgat gtccgagatt tcctgggcat
     1321 tggccggtga tacggctgct tccgggagat ggaacatggc cggattggtt tcatagtctg
     1381 tctgatactg taagagcagg tctttatcgg tcttgacata agtcggaccg gtaatggcct
     1441 ttaatttttc aatgatttct gccgtaatag gctgataagt tttcaagcga agggcctcct
     1501 tataaaaatc ggttgtctta ttttaccacg cccgctttgc ttagggaagg catcagaact
     1561 tgaaatcgat tgttgccgag acgccgacgc cctggacgcg gctgaggttg gtgacattcg
     1621 tcgtcgccat tgggatgagg gcattggccc gggcgatgcc gttgaattcc ccttccagct
     1681 gagcgacggc cttgaccttt tcgacctgtg ccgatgggcc gatgacctgg acggcgccga
     1741 tatatttacc
//
LOCUS       DAWCYS010000371         1744 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3140_length_1744_cov_15.120189, whole genome shotgun sequence.
ACCESSION   DAWCYS010000371 DAWCYS010000000
VERSION     DAWCYS010000371.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3140_length_1744_cov_15.120189"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          420 a          423 c          447 g          454 t
ORIGIN      
        1 ttgaactttt ttaaattcaa acttcctgct gcgtcagggc cttgccccga cagctcattc
       61 agtatactac tgtgtaaagg aattgtcaag gattttttta aaatccttga cgcaggccgc
      121 cgcgacatca cagatgtcgc tgtaggggcc ggccaaaggg cggcccgttg tgggaatccc
      181 gtgaaatgct ttgtagcgaa atcgccggtc gcaaaaccat gccacatata caggctaagt
      241 atatccccca ttgttcgcag cgggctgcct ttcaggacag cccctacgca caaggccatg
      301 ggccgtgaat gatttgatgt gaatccataa cattaacatc gcctggctgc gattccgtag
      361 gtgacgccca aagccctttc cagcccgtcg gttgccgacc aatgggaggc gcaagacggt
      421 tgctggaacg gacagcagag gctgggcgtc ccgttgtggg aatcccgcga aatgcgcttt
      481 gtcgaaatcg taattcgcag gccgcagttc gccggtcgca aaaccatgct acattgactt
      541 gacaagtatt ttccccatta ttcacagcgg gtcgcccaag gctcccttca taggggagct
      601 ggccgccaaa ggcggtctga gaggttaaag gacgtcccct acgatggccc cggttggggc
      661 gtaagccgcc cctgcgaaga agagggggac cgcttgcggt ggaggggttc tcctctacga
      721 gccgaggggt actcgaggcg atgttgtcag tccgagtcag tcgcggcggg acgccgcatc
      781 tgaatcgtcg tcgatgaacg atgaacggcc tacgacgaac gacatcgcca atgacgatgg
      841 gctccggcct gcggcctgca aaaaaagggc ttgccgcgtg tgcggcaagc ccttttttat
      901 cccttaaaat tccgctttcc aaataccgtg gagattgcac gattcgtata cgacgccgga
      961 gtcgacggct gcgaatacgg ctttcggttc ttcaccgggt ttgaggaatt tgacttccag
     1021 tttatcatcc gttgccaagg caatccattc gatgtggtgt tcttccaaca tcgggtggat
     1081 gacagagccg acagtgactt ccagtttatc gccgacgcgt tttacgacgg ggatgtgttt
     1141 ttctttagct gcatcaacgg aaccgacggc aatgggttca gcgccttcta cgctgccgct
     1201 gagcaaagcc aatacgtttt taccttcgag ctgatagaat tttacgtctt tcatgataac
     1261 tcctcctcta acaaaaaatt caagtgcata tataaggatt atatattata tgtcgttcat
     1321 aggtggtaat aaaatctttt gtacctttaa ttgatattgg ttctttgtta tgtatatatc
     1381 ttacatgatt tagccgtttt tgtcaagaaa taatcaacaa ataattatta tttattcgta
     1441 aatcaaaaaa aaaaagaaaa gagcctcaca tgatgggcgg aagtttcatg tgaggtccct
     1501 ttctctggag cgttatctta tatactatta ttctattata tgtacttcta tatgtacttc
     1561 aggaagaaag acggtgagcc ggcttatcgg aggatagcgc cggaaatgac catctgctgt
     1621 acctggttgg tgccttcgta aatctgagtg atcttagcat cgcgcatgta gcgttcaacc
     1681 gggtattcac gagtgaagcc atagccgccg aatacctgga cagcatcggt ggtgacctgc
     1741 atag
//
LOCUS       DAWCYS010000372         1739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3151_length_1739_cov_5.365796, whole genome shotgun sequence.
ACCESSION   DAWCYS010000372 DAWCYS010000000
VERSION     DAWCYS010000372.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_1739_cov_5.365796"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          418 a          528 c          461 g          332 t
ORIGIN      
        1 aggctccctt tataggggag ctgtcagcgt cagctgactg agaggttgaa tttgcattca
       61 cgtgtaaggt tcaagtttgt agtttttagt ttgtagacca tggttaaaaa tttaaaacca
      121 tctcctgcaa actacaaact ccgtactaca aactataaag agaaggacca cgaaccacga
      181 gcccctagcc acgagccacg attcgacggg attcccacaa gcgggccgcc tttacaggac
      241 ggcccctact agccactaat ccccaaccat ggtatgatat aaggtacagt ctatttcaga
      301 aagagagatg ccttatgaaa ctttttattg ccgaaaagcc gagcatggcc cgggaattgg
      361 cgaagtgcct gcctgatccg caccagcggg ggaacggctg tatccgcacg ggcggcggca
      421 tcgtgacctg ggcttatggc cacgtcctgc agcaggccga gcctcaggat tatgatccca
      481 agtacaagcg ctggaacgcc gacgacttgc cgattatccc caagcagtgg aagctcctgg
      541 tcagcctggc cagtaaggac cagttcaagg tcattacgga cctcatcgcc aaccccgacg
      601 tcgatactat cgtcaacgcc ggtgacccgg accgggaagg gcagctgctc atcgatgaga
      661 tcctcgatta cgtcggcaat aagaaacctg tcgaacgcat gctcttgaat gccctcgact
      721 agaagagcat cctcgaatcc ctcgacgatt ggcgcagcaa cggcgatttc gtccatctca
      781 cggaatcggc cctggcccgc agccgcgccg actggctcat cggcatgaat ctgtcccggg
      841 cctataccct ggccgcccgc cgtcagggac accgcgtcgt ctttcccatc ggccgcgtca
      901 agacgccgac cctggccctc gtcgtccgcc gtcagcggga attggacaac ttcgtccccg
      961 tcacgtatta cgtcgtcaaa gccgatttcc ttcataagaa cggcctcatc cgcgcccagt
     1021 ggcagcccag ggatacccag agcggcctcg aaccggaagg gcgttgtgtc gatgaaaaga
     1081 cggccaagtc catcctcgaa cgcctgcagg aacggcctga cggaaccatc gtcgagcata
     1141 aaaagaccaa gaaaaaagaa gcccagcgcc tgcccctgtc cctgtcgtcc ctgcaggtcc
     1201 tggcaggcag gcgttatggc tatacaccgc agcaggttct cgatacggct caggaattat
     1261 atgaacggaa actgacgacc tatccccggt cggactgcga atacctgccg gtgaaccagc
     1321 gcaaggatgt accccatatt ttggccaatc tcgggaaact gccggcaggg aagctggctg
     1381 aatgggtcgc cggtgccgac ggcaagatac gcagccgcgc ctggaacgac agcaagatca
     1441 cggcccatca cgccatcatt ccgacgaccg tagcctgtcc cttcgataaa ttgacgtcta
     1501 tgcagcagca tatttattac ctcgtcgccc aggcctatat tgcccaattc tatcccatcc
     1561 atgaatatga ccagacgcgc atggtcatcg aagcggctga tgaacgcttc gtcgcccatg
     1621 gcaagacggt caccgtgccg ggctggaagg tcctctatca gagcgacaag cacgatgacg
     1681 acgaagaaga gaaggggacc ctgccggctg tccgcaaggg cgatggcgtc cattatcag
//
LOCUS       DAWCYS010000373         1736 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3159_length_1736_cov_3.945271, whole genome shotgun sequence.
ACCESSION   DAWCYS010000373 DAWCYS010000000
VERSION     DAWCYS010000373.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3159_length_1736_cov_3.945271"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          345 a          529 c          448 g          414 t
ORIGIN      
        1 cgtctctgtc gtcaaggaac aacacgccaa ggccaagaaa atcctcgaag agcatcgccc
       61 gtccctggat gccctggcca aatacctcta cgaaaaagaa acgattaccg gcaaagaatt
      121 catggatatt ttgagagcgc agaccgcagg tcgtgactag tggctcgtgg ctcgtggttc
      181 gtggttcgtg gctcgtggtt cgtggttcgt ggctcgtagg ggcgccacgt ggggcgcccg
      241 ccatgaataa tggcgaatac gtggctatcg gctgactccc ctttgggacg gcccccacga
      301 acggctaaca cttcactata tacaaggtct ggcaaatgtg ctaaaattaa ttgttatgtt
      361 ggtaaaaatt ttatagagtt aaggggttat catggcaaac aagtttatct ccggcacatt
      421 gatcctgacc ttgtcgggct ttgtcgtcaa agccattggc agtatcaact ggatcatctt
      481 gtcccgtatt ctcggcggtg aaggcatcgg catctatcag atggctttcc cgatttacct
      541 gctggcactc gatatttcca gtgccgggct gccgattgca atttccatca ttacagctga
      601 aaaagtagcc aaacaggact atttcggcgc ccagcgcatt ttccacgtgt ccttgtccat
      661 gctctgtacg acggcacttt ttttgagtat cctcatgttc tttggttcca gcttcctcat
      721 cgatgaacac atcatccgcg aaagccgggc ctattattcg ctcatcgccc tggcaccggc
      781 catcttcttt acgacgatca ttgccggcta ccgcggctat ctgcagggct ggcagcagat
      841 gacaccgaca gccatttccc agatcgttga acagctggtc cgcgtcgtcg tcatgctcgg
      901 cttcgccgcg ctcctcctgc cgtacggcct cgattatgcc gccggcggtg ccagtctcgg
      961 cgccggcgcc ggtgggtttg cagcctggct ggtcctggtc tattactatt ataagttgaa
     1021 gcggaccctc cccgtcgacg ggccgacatt cgagcaggaa agcatcaagc acatcctcaa
     1081 gcgcctggtc atcctggcta tccccatttc cctgtcgagc atcatgctcc ccgtcgtatc
     1141 caacctggac ctgctcatcg taccgcggcg gctggaagta gccggttatt cgacccatca
     1201 ggccacggag ctgttcggtt acctgacggg catgtccgta ccgctcatca acctggcaac
     1261 gatcctgacg gcagccatgg ccatgagccg ggtgccggcc atcagccaca gtttcacctt
     1321 gaaggaatac gatgaaatcc gcgaacggac ggcgacgtcc atgcgcatct ccttcctggt
     1381 caccattccc ttcagcgtca tcctctacgt catggccgaa ccgattgtca ccttcatcta
     1441 caacgcgccg gccgctacgg atgccacgcg ggccgtggcc atcgccatct gcttcttggg
     1501 cctgcaccag ctttcgacgg ctatccttca gggcctgaag aaaccgaaga tcccggtcat
     1561 caacatggct gtcgcctgtg tcgccaaggt cctgtgcaac tggggcttag tcgccatccc
     1621 gaccctgggc atcaccgggg cttcctaggc gacagtggcc gacctcggcg tcgctgccgt
     1681 ccgcaacatg ttcgtcattt accgtaaaat cggttacgtt agcgtcatcg aagtcg
//
LOCUS       DAWCYS010000374         1734 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3163_length_1734_cov_5.375819, whole genome shotgun sequence.
ACCESSION   DAWCYS010000374 DAWCYS010000000
VERSION     DAWCYS010000374.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3163_length_1734_cov_5.375819"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1045..1054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          451 a          489 c          451 g          333 t
ORIGIN      
        1 gccatcctcg ccgaaaaggt agccgaagac aacgccgccc tcggtgagac ggacagcttc
       61 ttcactgtgg aacagctgga agaagccatg cagaaagtca tggataccta cgccggcggc
      121 atcggcagcc attaccagta caacgaaaag cagctggccc tggccgatga aaagatcgac
      181 cagctcatgg aactggccgc ccacgtcggc gccagcgatt accatgaact gctcttcgtc
      241 tacgaactgc gcgaacggct gacggtctgc aaggtcctca tcgcccacct gcgggcccgc
      301 aaggaaacgc ggtggcattc ctttgcagag aacctcgatt atccggaaca gcgtgcggat
      361 tggctgaaat acgtcaattc ccgcatggac gaagacggcc aggtccacat gatctaccgc
      421 gatttagtac caggagggga aacttatgag catacgcatt aagcaggaac gctgtatcgg
      481 ctgcggccag tgcatcgacg tctgcccggg cagcctcatc gaccgcaacg gccagggcaa
      541 ggcctggatg cattatccca aggactgctg gggctgtgta tcctgcgtca aagtctgccc
      601 ggtccaggcc atcgacttct acctcggcgc cgatatgggc ggccgcggca gcacgatgaa
      661 cgtcactgct gacggccact acctgcattg gcatatccat aaggccgacg gcagtgaaaa
      721 gaccattacc gtcgaccgca aacaggcgaa ttcatattaa ctagtttgcc gtttgtagta
      781 gcgagtttgt agtaaaagaa cacccattac tgcaagctac aaactacgaa ctgaaaactg
      841 cattttatca atagggggat tcatattatg tgtgcattat cgcatctcga caagttagag
      901 gccgaagcga tttacatcat ccgcgaagtc gctgcggaat gtgaaaagcc ggtcatgctc
      961 tattctatcg gcaaggacag ttctgtcatg cttcacctgg cccggaagga cattgatccg
     1021 gacatgacgc ctgtcccgat cctannnnnn nnnntcctcc atgtcaatac gacgtgtaag
     1081 ttcaaggaaa tgatgcgctt ccgcgatgaa accatgaaga agtacggcct gcagctcatt
     1141 gaatacatca accaggaagg cgtcaaacag ggcgtcaatc ctttcgatta cggcgcagcc
     1201 tatacggaca tcatgaagac ccaggccctc aagcaggccc tgcacaaata cggcttcacc
     1261 gctgcttttg gcggcggccg ccgggacgaa gaaaaatccc gcgccaaaga acgtatcttt
     1321 tccttccgca atgaagccca ggcctgggac ccgaagaacc agcgcccgga aatgtggaag
     1381 ctctacaata cgcacatcca taaaggcgaa agcatgcgcg tattccccat ttccaactgg
     1441 acggaaaaag atatctggca gtacatcaaa cgggaaaaca tcgacatcgt ctctttgtac
     1501 tttgccaagg aacggccctg cgtctatcgt gacggcaaca tcatcatggt cgacgacgac
     1561 cgcatgcgcc tcaagccggg agaagaaatc atgcacaaga agatccgctt ccggaccttg
     1621 ggctgctatc ctttgacagg gggcatcgaa tccgatgccg atacgctgga tgccatcatc
     1681 gatgaaacgc tgagcgccgt atcggcggaa cggacgagcc gcgtcatcga taat
//
LOCUS       DAWCYS010000375         1734 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3164_length_1734_cov_4.936867, whole genome shotgun sequence.
ACCESSION   DAWCYS010000375 DAWCYS010000000
VERSION     DAWCYS010000375.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_1734_cov_4.936867"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          395 a          494 c          488 g          357 t
ORIGIN      
        1 cttagtcctg gctttatggc gcgtccagtg ggtaatcacc gacttctatc aggaactgtt
       61 ccgcgtgagc ctcgccggtg atgtcgacgg tcttatcgag tatatttctt cttttggcat
      121 ctatgccgct ctcatcagta tcttcatgat cactatcacc gacatgaccg gcctgccgtc
      181 gattcccttt ctgaccgtca acggagccat cttcggtctc gtgccgggcc ttattatttc
      241 ctgggtcggt gaagtcctgg ggacggaact gagcttcctc atcttgcgga cggtcctgcg
      301 ccgccaggcc ctccacgtca tctcccgcca tcacttgcgg aatcagctcg ataaatacag
      361 ccgcttctcg acgctggcca tggcccggtc cattccgtat atccccaacg taccctttac
      421 ggctatcgcc gccgtcagcc acctcagcca gaaagaccac ttcctggcga cggctgtcgg
      481 caaggtcccg cgggtcgtca tcgaagtcct tttaggccat gacctcatcc acctcagcca
      541 gcacagcggg cggctgatgt tctgggtcat cctgacggtc ctgatctgct actgctggca
      601 ccgccatcag caggcttagc ggccgaagag gaccatgatg atgccgccca gggtcggcat
      661 ggcgacgata gctgccttga gcaggggctt aggggctaag tgcgtgaact ttgcacctat
      721 ataggcgccg agactcaggc ccagcagggt ctggacgaaa atccacaggt ccaggcggcc
      781 attgaacaag tagctcaagc cgccggcagc cgaaataggc aggacgatca gcatgcaggt
      841 cccgatagcg tggagcaggg aaatgccgaa gacgaccatc agagccagct ggatgaaggc
      901 cgcggcaccg atgccgaagg caccggacag gaagccgacg atgagaccgg taatgatgcc
      961 gtagatatag agcttctttc ccgttaaggc tgtcgtccgc acgggaaagt gccaggtcag
     1021 ccaggcgtct tggtagaggc ggacatagag aatcagtccg gaagcgatga gcatgagcga
     1081 cgtcatcgtc gccagcagtt ccgacggcat gatgttggac acattggcgc cggcaatcga
     1141 gccgatcata ccgctgccgc cgatgatggc cccaggcttc acatcgactt catgttcccg
     1201 gtaatgactg accgtcccgg aaatcatggt aaaagtcatg gccgccaggg aaacggccag
     1261 ggccgtatgg acaggaaaac agaaaccgac cgtcagcggc gtaatcgtgc cgcctgctcc
     1321 gccagcaccg acaaagccga tgacggaacc taacagaatc atggcaacga aaatcataac
     1381 gaaaaatccc cctaatatat gatataataa cacttattat accatatatt aggggaaaga
     1441 aaggacgcaa cttatgaata ttaccgttta tctcggttcc cgcagcggga accggtcttg
     1501 ttatgctgat tatgcttatg cgttaggggc ttggattgcc cgccacgggc acaccctcgt
     1561 ttacggcggc agccggactg ggctcatggg gaaactggct gacggtgctt tgcaggccgg
     1621 aggccaggtc atcggcgtgg aaccgcagtt cttcatggat gaggaattac agcatgaagg
     1681 cttgacgaaa ctgattgtga cgccggatat gacgtcacgc aagcagcaga tgat
//
LOCUS       DAWCYS010000376         1729 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3176_length_1729_cov_4.502987, whole genome shotgun sequence.
ACCESSION   DAWCYS010000376 DAWCYS010000000
VERSION     DAWCYS010000376.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3176_length_1729_cov_4.502987"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          318 a          566 c          424 g          421 t
ORIGIN      
        1 gatatggata tggctttcaa tgaggcccgg tgcggcatag gccccatggg catcgatgac
       61 ttcgtgtcct tcatacgggc cgccgacgcc ggcgatgatg ccgtcggcgc tgacgatatc
      121 cccaggccgg atggtctgtg tatagacgtc gacgatctgg cagtttttaa ggacgacgtc
      181 ccccggttcc aagccccggg ctacggcaat acgccgtttc aaaagattcc gtgtcatagt
      241 cataatgtac tccttcctat cggtaaaggc gtaagatttt tatctttcgg actcattgta
      301 ccatagattt cctttttttg acaaaatagt tgcccaaaaa ggcagaacac cctataataa
      361 tgatatcagt tgatgtccgt cgtctgctat ttggcttcat gtatttccgg atggatgtca
      421 acttgtttta ttactaaagg agtgtgaatg tattatggaa aaccaaccgt ccttcctcga
      481 ccgtttcttc cacttgagcg aaaatggcac gacggtccgc acggaaatcc tggctggtat
      541 cacgaccttc atgaccatgg cctacatcct ggccgtcaac ccgaccatca tgagcgccgc
      601 cggcatggac aaaggtgccg tcctcacggc aaccgccctg gcttccctta tcgggacgct
      661 gtgcatggcc tttttcgcca actatccctt cgccctggca ccgggcatgg gcctgaacgc
      721 cttcttcgcc tttacggtcg tcctgcagat gggctacacc tgggaaatgg ccctggccgc
      781 cgtcttcttt gaaggcgtta tcttcatcat cctgtctttg accaatgtcc gcgaagctat
      841 cttcaacgcc atccccatga ccttgaagaa agccgtttct gccggcatcg gcctgttcat
      901 cgccctcatc ggcctgctga acgcccagat catcgtcgcc aacccggcaa ccaagattgc
      961 cctgttctcg ttcaaacagt ctgccgctac gggtaccttc cacactgtcg gcattacggt
     1021 tctcctggcc atgatcggca tcgtcttcac agccgtcctg atggtcaaga aagtccgcgg
     1081 caacatcctc tggggcatcc tctttacctg gatcctggcc attctttgcg aattgaccgg
     1141 cctctacgtg cccaatccgg aaatgaagat gttcagcgtc atccccgacc tgtccggcgg
     1201 cgccgctgcc tttgccccgg ccagcctgag ccccatcttc ggccagctcg acttcagccg
     1261 cgtctttagc ctggacttcc tggtcgtcat gttcgccttc ctcttcgtcg atatcttcga
     1321 taccctgggc acgctcatcg gcgtctcgtc caaggccaac atgctcgatg aacggggccg
     1381 cctgccgcgc atcaaaggcg ccctcctggc cgacgctgtc gccacgacgg tcggcgctgt
     1441 catcggcacc agtacgacga cgaccttcgt cgaaagcgct accggcgtca gcgaaggcgg
     1501 ccgcacgggc ctgacggcag tctgcgtcgc tgtcctcttc gccctgtccc tcttcctgtc
     1561 gccgttcttc atggccatcc cggcctttgc cacggcaccg gccctggtca tcgtcggcta
     1621 cctcatgcgg acgtccctcg ccggcatcga cttcgacgag ttgagcgaat ccatcccggc
     1681 atacatcacc atcatcgcga tgcccttctg ctacagcatc tccgaaggc
//
LOCUS       DAWCYS010000377         1726 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3182_length_1726_cov_3.569120, whole genome shotgun sequence.
ACCESSION   DAWCYS010000377 DAWCYS010000000
VERSION     DAWCYS010000377.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3182_length_1726_cov_3.569120"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          399 a          452 c          497 g          378 t
ORIGIN      
        1 ctgaacgaat tttcgtaggc cgcccgggac gactccgaca gagccggccc ttgcggcggg
       61 tgcccctgct cgtagatgtc ggcaaacgtc gctgtaagcc ctgttggctc gtgattgtat
      121 tttaaaagga aagtgagggc cgcatcatgt gtctcgaaat agcccaaggg tttttgatgt
      181 ccgttaatgg ttttctttac aacccacggc ctcctcctat taccctgtaa tttatagata
      241 gacccaaagc catttggtaa acgcattata atcactccgt ttttaactga gtgtaccatg
      301 aaaggacggc gaaaagaatt gctggaccaa atcatcgtag cagtcatcag ctacgtcatc
      361 ccggccgcta gtatcacaat gctcggctgg atcatcaagg ctttccgtaa ggataggact
      421 cgtcagcagg ctctagaaga ggggcttaag tgtctcctgc gcgaccgcat catcgcggcg
      481 caccactatc acatcatcga gggccacgaa gtgtcgcacg aggaatataa cgccatggcc
      541 gatatgatag cgagctatac ggcgctgaaa ggcgccaacg ggtacatcga gcgcattgcg
      601 aaagagtaca cggaagcgcc ggtcgccgac gcgatgcact gacagggggc ggcgctatga
      661 aagaaaaaat catccagctc ggccgctggg gccagcagaa ctggctgccg ctcatcatca
      721 tcctcagctg cgtaaggatg cttattctcg tggcggtcat ggtgagctgg ctggtcggat
      781 actgggccaa cgcgctgtac ggcatgcatt tcgagctctc cagctgctgg agcggcatca
      841 gcgttgtcgt gaccgggctg gccggggtcg tcgccctggc aggcgcctgt tggacgaaat
      901 atcacacaga cagtaaattc aacacgccgg cggggcttcc gccggtcgta tcccgtatcg
      961 tagacaaggg aggtaacgca aaatgatgga aggtatcgat gtaagctact gccaggcagg
     1021 ctttgatttc caggccgcgg cagatgaagg gaaagaattc tgtatcgtcc gtatcggacg
     1081 tacgcgtagg gatggccagc aggagctgga cgactacttt gtcgataata tcaacggcgc
     1141 gaaagcggcg ggaatgaagc tcggcatcta tttttattcg cttgcgacga cggaagccga
     1201 agcggcgcag gaggccgagt ggctcttaga cacgatgcat gagtaccttg acggcgtatc
     1261 gctcgaagcc ggcatctggc ttgacgtcga agcgccctgt cagctcgctc tgagccctgc
     1321 cgatctgacg gcgacggtca tgagctgggt caacacgatg aacgcagcgg gcgtctatgt
     1381 cggcatctac tcgtattact cgatgttcaa cgacaacttc gaactcggca gcatcccggg
     1441 ctacgtcccg ctttgggtcg caaacaccgc gtcggtcaac tacctcaagg ccgagaaccc
     1501 ggaactcaac atcccggttt ggcaatactc cgatgcgggg aatatcgctg gcatcaacgt
     1561 cgacctggat gtttggtatt gaggtggtga tacaatggac cgaaaagagg tcgtcctcca
     1621 atttttccgg gaaaactgga aattctggct ttctgtgtgc ctggctgtcg cgtttttcat
     1681 tatcgcggtg acagtttaca tgctcaaaga agatagagtc gtcaca
//
LOCUS       DAWCYS010000378         1726 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3181_length_1726_cov_4.944345, whole genome shotgun sequence.
ACCESSION   DAWCYS010000378 DAWCYS010000000
VERSION     DAWCYS010000378.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_1726_cov_4.944345"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          382 a          462 c          550 g          332 t
ORIGIN      
        1 ccggccgtgg gcgggcgacc agggtgcctc cgctggcgtc gaagtgggac gcgatggcca
       61 ctccaatggc ctggccgtca tagcggggca cggtcttttc gccgccgccc aagggataga
      121 tgacggtgat cttgggatga taattattga aatcgttgct ggcccgcgtg atgagcagca
      181 ggcccagctg gtcggcaccg gggaaggagc cgaaaaccgt cttggggatg cccgtaccgg
      241 ccattttcag gtacttcgat tccagcgacg actgggtgct gacggaatta tcgtcgtgcc
      301 ccaggctgta ataggtaaag acgcctttgc gggcatcatc gatgaggcgg taattgatgc
      361 tcatgttctt gcggcgccgg ttataccagt cctggaggta ttccatgggc acgcggcgca
      421 tgatggcgaa ccagtcattg cgttcctgcg gattcaggcc ttcttcgtcc atcttggcct
      481 ggagcgatgc cagctgatag atgtcggagc ccatggtcag gtaatagtcc ggttcgacgc
      541 ccttgccgcc ggcccaggga ctgcgcatga ccgtgctgaa gacataaatg ggcacgttct
      601 tgtgcgattt atgcagcgct tcgacctttt ccaggcgcgc tgtcagcgtt tccatcggca
      661 ggttatgctt gcgcgaatcg acgaggccgc catagaccag ggaatcgatg gacaggacca
      721 tggcatcggc cttgccggca ttggcattga cccaggccat gagcttatcc ggcgaaccgt
      781 ggaagttcat gcccgacaga tactgggccg gcggcgtcag gacctgatag cccgccgctt
      841 cggccgtact gaccgtgtat tcataatcga caggccggtc atcctggggt acatataaaa
      901 tcgtctttgc cagtgcagcc ggcgaggcac ataaaaagaa agaggctacg gccaggctga
      961 ctgctttctg taacagattc acgttatcac tcctattatg aagaaaaaaa ttgtaccctt
     1021 tattatatca cggccctggg aaaatttgta ggggcgccct acgccctccg atggtgggcc
     1081 cggcgaatcc ttgggatatt gaggtgcagt cggatggcca ccaggcggat ggcgacggtg
     1141 acggaaaagg cgacgatggc gccgacttcc aaggggacct caaaggggat gacggcgccg
     1201 tacaagaaga tactgcccag gagggacgcc gtcgcgtaga cttccttgcg caggacgccg
     1261 gggatacgcc cggccaggac gtcgcggatg acgccgccgc cgacagctgt caggacgccg
     1321 agggtgatgt cgaggatcca gtagtcggga taatagaagc agcccaaaag ggtcccggta
     1381 accgtaaagg agccgaggcc gatggcgtcg cagacgagat accagatgga gagcttgccg
     1441 acgacgcggc gccacatgcg gatattgaaa tagcggacga tgatcgtgat gacggccatg
     1501 gagccgagga tgatccacaa atagatactg ttcttaaagg cagccggcgg gatattgccg
     1561 atgaggatgt cgcggatgat accgccgccg acagccgtcg ccgcactgag gacgaagatg
     1621 ccgaagaggt ccatgcgccg tgaaatggct gcgaagacgc cggatacggc aaaggccgtc
     1681 gtccctaata agtcgaaaat ctgccaccaa atggcatcca taatag
//
LOCUS       DAWCYS010000379         1721 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3197_length_1721_cov_5.541417, whole genome shotgun sequence.
ACCESSION   DAWCYS010000379 DAWCYS010000000
VERSION     DAWCYS010000379.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3197_length_1721_cov_5.541417"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          316 a          497 c          565 g          343 t
ORIGIN      
        1 tcccaccaca gtgcggtgag gtagacgccg tctgggggga tggccatgtt gatggggatg
       61 agcatgttgg ccgtagcgaa accgatggtc ctcccccgcc ggtcgccggg cacgatggtg
      121 ccggtcaggg caaagggacg gcccaggaga ttctcggccg tttccatgtg gcccatctgg
      181 atgagacggc ggatgacggt gctgctgatc ggtgtcgtcc ggcccggccg ttcgaggagg
      241 gtcagggcga caacggggat gccgtactgg ctcatgaaga atttcatggt ctccgtattg
      301 cctgccgcct tggcaccata ggtaaagttc gagccgacga cgatggccga cggatggatg
      361 taggtcagga ggttctggca gaactcttcc ggcgattcct gcaagagggt ctggctcatg
      421 ggcaggagga ccaggccgtc gatgccgacg gcttcgatat attccgcttt ctggccgatt
      481 gtggccaggc gcagcggttc tttttccgga cacaacacgg acagcggatg ggccgtaaag
      541 gtgacgacca tggttttcac accgccggca gcggcccgtt tgatggccgt cttcatgacc
      601 atctggtggc cccggtggac gccgtcaaag gtcccaaggg ccacgacggc tccggtaaag
      661 ccggttattt cctgaaagga gtggaatatt tccatcgtta gttacctcac aatccctctg
      721 cgaacatctt atgaggccgc aggatatggt gttccccgtc gtagtgggca atgcccatga
      781 gccgctggtc cggcccgtac acgcggatga cgtcgccatc ggccaggtac ggcccggcga
      841 agggcacggg acggccctgg agcaggaaga cacactgttt ggcggagcaa ttcgcctttt
      901 taaggtcctg gatagccata tcggccggcc ggacataggc catgggatcc cgttccagct
      961 cttccagcgt cgccgcgtcg tcgatggtga acagcccggc ccgggtgcgg accagatagg
     1021 tcatgcaggc gcaggtcccc aggacctgac ccaggtcgcg gatgagggaa cgcacgtagg
     1081 tccctttgga acaggtcacg gcaaaggtgc cgcgccggcc gtcaaaggac agcaggcgca
     1141 ggtcgtggat ggtcacgtgg cgcacgggca gggtgaaatc gacgtcctgc cgggccagtt
     1201 tataggcctt gacgccgttg acgttgatgg cggaatattt ggacggtgcc tgttcgatgt
     1261 cgcccacaaa ggacgccagg gcgcggcgca cgtcgtcagc cgacagatcc ggtacggccg
     1321 ccgtttcgac gacggtcccc gtactgtctt ccgtatccgt cgccaaccct aaggcgaatt
     1381 cggcatggta tgatttgacg tcgctgtcac cgtattcgat gaggcgcgtc gcctgaccgg
     1441 catagaccgg caggacaccg gccgccaaag ggtccagggt gccggcgtgg cccatcttct
     1501 tcatgccata gatacggcgc aggcagccga tgacgtcgtg actggacatg cccgtcggtt
     1561 tcagtacatt gatgagtccg tgtatcatag gcgcttttcg atttccttta cgatgagttt
     1621 cttcgcttca tccaaaggct tatccaaggt gcagccggct gcgcggacgt ggccgccgcc
     1681 accgaagaca gcggctacgg cgttgacgtc catgcccttc g
//
LOCUS       DAWCYS010000380         1717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3206_length_1717_cov_4.371841, whole genome shotgun sequence.
ACCESSION   DAWCYS010000380 DAWCYS010000000
VERSION     DAWCYS010000380.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_1717_cov_4.371841"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          362 a          529 c          476 g          350 t
ORIGIN      
        1 ctgccccttg cgctcctcct agggcgccta attcttttat ggctgctcga aggtattacg
       61 cgccggctga tgccgaaacg ggcgttgtac tacatccagg tcacaggggc cgtcctcttg
      121 ggctccctgt tcctcttcgc catgtttcag gatatcagcc gcttttagaa aggcaggcct
      181 tgcatgaaac gtagaaaatc tcttcccgtc caggcgggca gcgtcaccat cggcggcgat
      241 gcacccgtat cggtccagac catgtcgacg gtcaacccgg cccatatggc cgaagccgtc
      301 gtcgatgccc gccgcctggc atcgtatggc gctgaaatct tgcgcttcgc cgttcccgat
      361 atggaagcgg ccaaaggcct ggccgccgtc gttcaggccg tggatatccc cattgtcgcc
      421 gatatccact tcgattaccg cctggccctg gccgccgtcg acagcggcgt ccaggccctg
      481 cgcatcaatc cgggcaatat cggatctgac gacaatgtcg tcaaagtcgt cgaaaaagta
      541 cgtcctaagg gaatccccat ccgtatcggc atcaatacag gttccctgcc ggaagatatc
      601 ctggctcaga gcggcggcca tccgacggca gaaggcatgg tcgaaggggc cctgcgccat
      661 atccgcatcc tggaacgcat ggattaccgc aacatggtca tttccctcaa gtcctccgac
      721 gtgcccttga tgatcgaagc ctatgaagcc ctggccgata aggtgcctta tgccctgcac
      781 ttgggcgtaa ccgaagccgg cctggtccgt gaagggagca tcaagtcggc catcggtatc
      841 ggtacgctgc tccacgaagg catcggcgat acgatccgcg tatccctgac gggcgatccc
      901 gtcgaagaaa tcaaggccgg ccagaccatc ctcagtgccc ttggcctgcg ccgtttcggg
      961 ccgaccctca tttcctgtcc gacttgcggc cggacccagg tccacctcat cgacctggcc
     1021 caggaagtgg aagaggccat ttctcatctg actgtgccca tcaaggtcgc cgtcatgggt
     1081 tgtgtcgtca acggtcccgg tgaagccagg gaagccgatt tcggcattgc cggcggtaag
     1141 gggaagggca tcgtcttttc tcatggccag gtcctgcgca ccgtgccgga agaccggctc
     1201 gtgtccagcc tgctggaaga aattgaaaaa accattgcaa aggagtctta actcatgtta
     1261 gcatcgaaat tgtatagtcc gacgctgcgg gaaatcccgg cagacgccga aatcgccagc
     1321 catcagtata tgttgaaagc cgggatgatc cgcaagaacg gcggcggctt gtattctttc
     1381 ctgcctttag gccgccgggt cgaactcaag atcgaagcca tcgtccgcga agaaatgaac
     1441 aacatcggcg cccaggaact catgatgccc attatgcagc cggctgaaat ctggcatgaa
     1501 tcgggccgct ggaatgccta tggcccggaa atgttcaaac tcgaagaccg ccatggcaat
     1561 catttctgcc tcgggccgac acacgaagaa ttgattacga ccctggtccg caatgaactg
     1621 cgctcgtaca agcagatgcc cgtcacgctg tggcagatgc agaataaata ccgcgacgaa
     1681 atccgcccgc gtttcggcct catgcgcagc cgtgagt
//
LOCUS       DAWCYS010000381         1717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3205_length_1717_cov_5.382070, whole genome shotgun sequence.
ACCESSION   DAWCYS010000381 DAWCYS010000000
VERSION     DAWCYS010000381.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_1717_cov_5.382070"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          438 a          335 c          472 g          472 t
ORIGIN      
        1 ccagcccgtg cccatgcaca ttttgccctt tacttcatgg ctgccgacga cgaattcgac
       61 gcggggattg tggctgacgt tcttttcagg cttgtggaaa ccgaagcagg ggatgaggat
      121 tttttcatca tccgttacga tgaggtattt gttccaggtg tttgcgatgt gtgcctggcc
      181 ttcggcattg gccgaagcga cagagacgat gccatcgtgt ttgatacaat cgaaaaagac
      241 gttggttaac atggggattc ctcctttagc aaaaaagact acgttcatta tagcaaaagg
      301 gaaggggaat gtatacggcc gtgagatatg attgggtatg gtactttact cataaattta
      361 tgagttactt attaatttct ttatatcttc cgcaaaaggt gtaaatttca tatactaagt
      421 gtgtcgatag aaagccatgg ctcagtttta gattatcttt atgttgggag agattatgat
      481 gaatgaagct gtaaagaata ttttgaaaaa ttgtatgtgg gatttggcta cgtgtgctaa
      541 gggcgaaccg aatgtcgttc ctgtggcttt taaggatatt accgacgatg gcaagctcgt
      601 tgtcggcgac gtattcttgc agacgacgtt ggataatttg aaggccaatg acggcaaaat
      661 cgctatttct gcctatgatg cacagaaatt ggaaggctat cagattaaag ggacggcggt
      721 ttatgtcacg gaaggtcctg tcgtcgatac attcaagaaa atcgtcgaag atatgttcaa
      781 aggcgctgct acggctaagg gcgccctggt catcacgccg gaacgcatca tcgttacgac
      841 gccaggtgct gataataaga aagtcatttg cggctaattg gcaatgatgt tatgagaaaa
      901 gggctgttgc atgagtatca gacttcatgc gcagctcttt tcttttttgc gggtcgccgg
      961 aaagtcttga aaatcggcgg cagaagggct aagatgcaaa cagaagggag gagagaatat
     1021 gtataagaaa aagctggaag aggacatccg ctgtcctttg gaatatggcc tggaactctt
     1081 tggcggcaaa tggaaatcgc gtatcatctg cgtcctggca gcgtgccgtg tcctgcgtta
     1141 cagtgagctg cgccacgaaa tgtataatat taccgacgcg gtcctggcga cgacgctcaa
     1201 gcagcttatc caggatggtc tccttgtccg caagtcatac gatgaagtgc cgccgcgggt
     1261 cgaatacagt ctgacagaaa aagggaagtc tgttgtaccg attctgcagt ctatttgtca
     1321 ttggtcgggt gctttttaca aggaagagac ggaccatccc atgacacagt gccaaaaatg
     1381 tgatttttcc tctaaagttt aagaaaatga ggctgtcttt gcgggacggt ctctttttgt
     1441 taaattttgt tgccacgagg cggtctgagg ttatataatt ataaatcata cattactttt
     1501 ttaggaggac gtatatggca gatattcgta tcatccgggg gcagcgaatc gtccccgacg
     1561 tggagcaggc tctgcggttt gctggttatg aatcgaaagg ggcagcttgg gaacgggctg
     1621 aagggatgtg ccgggatatg gcaccgctct tgcggcggtc tttgcaggcc aaggcggtcc
     1681 tggcctttac agagaaacgg ctctatgtca tcctgat
//
LOCUS       DAWCYS010000382         1704 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3230_length_1704_cov_4.735597, whole genome shotgun sequence.
ACCESSION   DAWCYS010000382 DAWCYS010000000
VERSION     DAWCYS010000382.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_1704_cov_4.735597"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          468 a          357 c          358 g          521 t
ORIGIN      
        1 cacgtcgttt tacgtagcga ccaggtatgt ttcatgacat aattcctggt acaacatctc
       61 tttttgagag atataatctt tgattaaatt tacgacgtct tctccatttt tgagtctacg
      121 aaacaacgat tcaaggaaat atgcatttac ttcaatttcc ttcttggtgt cctcacgcga
      181 tgcgagtcta cgttcaagtt ccttttttac ttcttctttt gctgtcatga tgtttgtctc
      241 ctttctactc cctgggactt ccttgtccct ctttctgatt atattgtaac acattttatc
      301 atgttgtcaa ctgtattttt taagttattt tatttctttc tgatttctat tgtgtagccg
      361 taccgactaa caatatcgtc taattcgata tactttatcg tagcgttcct ggtctttgcg
      421 ttgaatgtct gttgcttgag tccctgttct ttgcaaagtt ccgtttccga cttgccaatc
      481 tcgacaagcc atttcttcca cataatttta aattcttctt tcgtcatgat gttttctcct
      541 ttctgttatc tatagtatat ataactatgt ttaaaaagtc aataactttt aaaaattttt
      601 aaataactct atttttactg ttgacaagtg gttgaactcg tgatattatg tagacaacaa
      661 atgaaatgca gttacagaga ggagaaaata tcatgacaat gttaaagaaa aacacctaca
      721 ctatcgacgg agaagaattg cagaagagaa tctcgagtga aattgaatcg ctcaagtaca
      781 actaccgcga atggcggtta gctgaacctg gctcgcatga ttcggaaacc tgctacaccc
      841 gttacgaatg taatttagcg gttttaagag cgctgcttcg tatccaggga ttagacggca
      901 cacaggtggc aatgattata aacaacatca aggatttggc agaatacgac tccagtaact
      961 acagacgtag tctcttggcc ggaaaaattg acgccctagg cacacagctg taccacatcg
     1021 tcaaaacttt caaagtagag gacgacggtg aaagtgaaaa accgcggtat tatgcggtta
     1081 aagctcagcg agacgaatta attaagcgcc gtagcgtatt aatccggaaa attgctagat
     1141 agtagattgt tgtacggggc cgaaaggccc ctagaaagga tggataccat gacttgcaca
     1201 atcccgggca ttaaggccaa cttgaaagac cacgccgcat ataaggcgca aaggaaggct
     1261 gttgacgatg cttggaaaaa ggtattagct caaccgctcc aggctctcta ctttgacgac
     1321 gtctgcctca gccctgccac gaatggcagt ccgtatccgt ggcgggtaac ctgggtagat
     1381 ggggataccc ctatttgcca tgtgatgctc gacagcctgg aaaaggcctt ttgggaattg
     1441 cgcggctaca ctggccgcac ggtaacagtt gttttttagg aaggatgata actatgaaga
     1501 agacttatta cttttactac atgctcgaac gtcctgtatc tattggtact cagcccgata
     1561 agttcataag ctttacggat gagcagggca tgacgccgtc tgatcatcac cattggggca
     1621 ttgtctacta tgaccgccct ttgagtgatt aagaaatgaa tgactatgaa atggaatacg
     1681 gcggcgttac agatagtttg ccgt
//
LOCUS       DAWCYS010000383         1695 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3247_length_1695_cov_5.526829, whole genome shotgun sequence.
ACCESSION   DAWCYS010000383 DAWCYS010000000
VERSION     DAWCYS010000383.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_1695_cov_5.526829"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          438 a          404 c          432 g          421 t
ORIGIN      
        1 atacgagcca ggagtccgtc tcgggggctc ggagatgtgt atacgcgaca gaataagtag
       61 aggcgcgcac gtgacgagcc cgccggccgc agttcgcagt tttgcctccc ctgatagggg
      121 aggtggcctg aaaggccgga gaggttgggg ttagctataa gctgatggct agccactgac
      181 aactaacaga tatgggcgcc ccgcgtggcg cccctacagt aaaagggctt gtcgaagtac
      241 gacaagccct tttactttgt acttctttga acttagtctt ttcctcagaa aggagccttg
      301 tatgaaagca tttaagccat ggccggaccg tatcgtccgc cagaacacct gtatgaatac
      361 gcgggcttgt gccgtcttag tgccgattat ctacaaagac ggggaagaac aggccgtgtt
      421 tgaagtgcgc agcagcaagc tgggctggca gcctggcgac gtctgcttcc ccggcggccg
      481 catcgacgac ggcgacgaca gcgctctgga agcggccctg cgggagacga aggaggaact
      541 cggcgtcgac ccatcccata tccacgtctg gggccccctg gactacatgg aatcacctgt
      601 aggcgtcacc gtatggccct ttgccgccta tatggataca gatgacttcg tcctcaaccg
      661 gggcgaagtg gaccatacct ttacggtccc tctgtcctgg ttcgatgagc atgaaccgga
      721 aatcggccgc gtcgaactgg ctacccggcc ggcaccgggc tttcccaagg gcctggatat
      781 ttcccatcag acaggctgga aacagcgccg gacgtataac gtcaagattt atacgtatga
      841 aaattataaa atctggggaa ttacggcaca catccttgac aacttccggg gtatccgtgc
      901 tataatacaa ccgtaagctt tccttaatat ttgtaaccag tatagttagg tatacgtaca
      961 aaagcgtata cggaaaagca aggcgtactc tgtcaagaag attttaatct ttttgcctcc
     1021 attagtacaa aaaagtcttg ttaaagtttt aggatgagaa aatggaatag tgatattgtg
     1081 ctttataggc tttttgctat tgatttaaca tgtgaaaagt tgtacaatgc atttgtaagc
     1141 actgagacgg tgtgaatata tatccagaca gaatttactg taaggaaatg tatcgcctcg
     1201 atgttcaggt ttttatctta tggctttact aaactaacta tacactaagg agagtggaaa
     1261 catcatggat tttaacttaa cagatattca acaggacttc ttaaaactcg ctcatgattt
     1321 cggcgaaaag aaattagctc cgaccattac ggaacgcgac cacaaaggta tttatgacaa
     1381 agaccttatt gatgaactgc tcagcctcgg tattaccggc gcttacttcg aagaaaaata
     1441 cggcggttcc ggcgatgacg gcggcgacgt tttgagctac atcctcgctg ttgaagaatt
     1501 ggctaaatac gacgctggtg ttgctatcac cctgtcggca acggtttccc tttgcgctaa
     1561 cccgatttgg cagttcggta cagaagctca gaaagaaaaa ttcctcgttc cttggttgaa
     1621 ggtactaaaa tcggtgcttt cggctagacc gaaccgctct ctggtactgc tgtcaattct
     1681 acacagctgc cgctg
//
LOCUS       DAWCYS010000384         1693 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3254_length_1693_cov_3.778999, whole genome shotgun sequence.
ACCESSION   DAWCYS010000384 DAWCYS010000000
VERSION     DAWCYS010000384.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_1693_cov_3.778999"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          613 a          372 c          396 g          312 t
ORIGIN      
        1 gataataaca gcaagaacat catgaacgca atgattgccg ctgcaccgga agtcgcgatg
       61 gtcaacgaag gtatcaagaa ctctaccttg tatcctgatt atgatgtttc cgacgtcatc
      121 acgaagacgg ccaagactat catgtcgctc cgtaatgaag gcaaaccgct gtccttccac
      181 ttgcaggaaa ccagcctgtt ctcgcaggga gaatcggaag ccgaacggct ggtacttgaa
      241 ttcatcgaac gtaataaatt caagagccgt accattgccg atatgtacaa aggggcttgc
      301 gaccgtatct ttgccatcgg tagtacgaaa caatccaagc tgttcgacag caaggaagcg
      361 ccacgtatca gccttgaaaa catcatctcg aacgccatcc aggaggtagc acatggacaa
      421 tcgttattcg acaccacaga agaaaagcca gccgaaaaag ctgtatccga agtatcagat
      481 aatcgacagg ccgaacccgc cggaagtgga cgcgtacatc aacaagaagc tggcagagta
      541 cagagccaag aaaaagaagg gaatgaagta gatgaaaaga gcaagcaaag tgaccatgtc
      601 gacaccgaac ctcagcaaac tgaaagtaaa gacaaggaaa gtacccatgc cgaagaaggt
      661 tcccaaggtg acgtacaaga agaaataagt aaattccata atgtactgga tgacgaaaaa
      721 tcaacgccta aacaggttat agacgcttat aaaagtgtcg ttgataaagt catcgcaagt
      781 gccgacggaa gccgtaaaaa cgccaaaatg ggcgataaaa tcgtaaccga cgaataccag
      841 tctttgacta attccaagca ttggaacgca ttcgtgaacg aagacggcgg acggaactgg
      901 catgaagcca ctatcatcaa cgcagaagct cacaagaaat tacatactat catcaaaact
      961 gcaggtgtaa aaaccaaaga agcaccatca tctcaaggag ttgcacctta tagacaagcc
     1021 gtagatgata tcatggataa gctggctcat aagaagctta ccccggcgca ggctatcaat
     1081 aagctaaaaa ccattatgaa ggatgctaat gccgctcgtt ggggagaatt caaagccatg
     1141 gaagccatcg agtcgaaaga aaagttatcg ttcgatgatt ataaaatggc actcgattta
     1201 gccaatgacg ccatgaaaaa agccaatgac atgcgtctcg aagccagccg taaacgcaag
     1261 gcccggaaga cggagaaaga attggaatcc gctacggaag aaacaaagtc ggcagaacag
     1321 ccaaaagaag aatccggatt cactgatgag gaaatcaagg ctctcacgga cagaggcttc
     1381 aaccgctgga cgaagaaact gcctaatggc aaggtaatgg accgcctcga tattaaaccg
     1441 gaatatttag ggcttgaact tacccggtat aaatctggca acatttcatc tgcaaaattc
     1501 aacggggaaa caatcagtaa ctcagaagca agacgcatag aaggtactaa atgctatgta
     1561 gacgttgcga caaaagaagt cgtatgtgac agagcagacc tgaaaaacgc ggcacaagaa
     1621 gtcattgacg atgcgttatc aaaagaaaaa ttcaagtaag cggaaaaact aaagaaatat
     1681 tcagtacggg aag
//
LOCUS       DAWCYS010000385         1689 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3263_length_1689_cov_9.287026, whole genome shotgun sequence.
ACCESSION   DAWCYS010000385 DAWCYS010000000
VERSION     DAWCYS010000385.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_1689_cov_9.287026"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          510 c          392 g          449 t
ORIGIN      
        1 aaaaagagag ctgtcgcaca aagccgaaat ggcttaatgc gacagcccct tttttctgta
       61 ggggcgacca cgtggggcgc ccgtttacgc aaatcggttc atggatattc tacatatccc
      121 cagcgggctc gccacgtgcg agcccctaca ggcacaaatc acgagccact gtcaataatg
      181 cacaaagtgc attgttgacc attgataata tactcaaaat tctctttgcg caaaggaaat
      241 gcccgtttta taacttttta ttatttttat tatgaattta tatcatgctg ttttcgtaat
      301 ctttactata agtaacttga aattttctct cctttagcct atagtaagtg tataagtaca
      361 gtgtatactt tatttctcgt gcgatacata attcatatta caccaattag aaataagtat
      421 agctccggat ccgtgggaag tagagtagcc tacacacaat ttcataaagg agggattgtt
      481 ttatgaatgg tttgtattta gtcattatct ctgcattagt atttgtactg gcttaccgtt
      541 tctatggcgc tttcatcgct gccaaagtct tgacggtcaa ccagtacaag gtcacgccgg
      601 ccttccgctt tgaagacggc cacgactacg tgcctaccaa caagtacgtc gtcttcggcc
      661 atcacttcgc cgctatcgcc ggtgccggcc cgctcgtagg cccggtcatt gccgctcagt
      721 tcggttatct gcccggtgcc ttgtggatcc tcatcggtgg cgtcctcgcc ggtgccgtcc
      781 acgacatggt catcctcttc tgctccatgc gttacgatgg caaatccatc gctgaaatcg
      841 caaaagccca gattggtgac aagactggct tggctacgtc cttcgccgtc ctgttcctca
      901 acatcctggc tatggccggg atggccgtcg tcatcgtcaa cgccctgcac gacagcccct
      961 ggggtacctt caccatcggc atgaccattc ctatcgctat cttcatcggt atttatatgc
     1021 agttcctgcg tcctggccgc atcaaagaag gcactgtcat cggcgtcgcc ctgaccttgc
     1081 tggccgtcgt cctcggccct gccgtccagg catcgcctac tttggctccg ctcttcacca
     1141 tcagcgccaa agggatttcc atcgctctca tcatttacgg cttcgtagcc gctgcccttc
     1201 cggtatggct gctcctggct ccgcgtgatt acctctcgac atacatgaag atcgggacca
     1261 ttggcgccct ggctatcggc atcatcatcg tcggcccgac cgtccagatg ccggctatca
     1321 cccagttctg cgccggcggc ggcccggtca tcaccggccc tgtcctgccg tacatcttca
     1381 tcaccatcgc ctgcggtgcc gtctccggct tccactgcat gatctctacg ggcacgacgc
     1441 cgaagatgct catgaacgaa cgctccatcc tgcccgtcgg ctacggtgct atgctgaccg
     1501 aatccttcgt cggcatgatg gctttgattg cagcctgctc gctcgtaccg aatgactact
     1561 tcgccatcaa ctcgtcggca gaacatttcc aggccctggg catccaggtc gtcgacctgc
     1621 cgcagctgaa cgccatggtc ggtgaaaacg tcgcccatcg tccgggcggt gccgtttccc
     1681 tggctgtcg
//
LOCUS       DAWCYS010000386         1686 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_3406_length_1686_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000386 DAWCYS010000000
VERSION     DAWCYS010000386.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406_length_1686_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          299 a          439 c          512 g          436 t
ORIGIN      
        1 gccgtcttct tggtggagtc ggtcagctga ttgcaggcgc tgttgatgat atacggtacg
       61 ccgttgatag taccagtatt gccctggctc tggatgtcgt agaacttctg tttggtggaa
      121 gtacgtacgg atgcgaaggc cagcaggtcg agcttgttca ggatgaggac ggctgcgtct
      181 tcgacgtctt ccttgccgcc atagttaaag atgatctggt cgagggtggt atcatcgata
      241 ccggccaggg tgaggtcgga agccgggtcg atggccgtgg ccttgtcggc gaagatgccg
      301 acgaaggaat tggtggcgcc gctgccgagc aggatttctt tcgtcatctg cgcacggata
      361 gaggtgcgga tggcgttctg tacgtagccg gcgtaattgg cgttcggcag tttgagcatt
      421 tcgcgggaga tttcgctgta cgcggtgatc ttcgatttcg tcatggctgc cttgccaaag
      481 gtgacgtcag tatcggccgg cgtaccgcct tcagtggtgt acgtgccttc ggggatgtcg
      541 atacgatacg gttcttcata agattcgccg ccgttgaggt tgacgtgtct gactcggtcc
      601 accagggacg atacgacgtt gaagccttcg ttgatgaccg ggctgtcaaa ggtcgggacg
      661 atgatcgtcg gcgtcgatgt caagatggaa cgtttttcgt gcgggaacaa gccttcggcc
      721 gcgaagcgga cgaccgtgcc gcgctgtttg aggtccgcgc cgcgtttttc catttccgcc
      781 tgggcgtcct gtgcgcgttt atccgtagct tccggcgcag tgaaagcacc tttcgggaca
      841 tcagcgcgtt tttcggcttc cttgtgcacg gcggcagtac gatcatcgac aacaggagct
      901 gccggagccg gttccgccgg tttagctcct tcgacatgag cgtcacggcc ttcctggtcc
      961 gctttagcgg ccttcagaat ttcgatgttg ctgcggagag tctgcatttc acgatagatg
     1021 gagcggagtt cggcgacgtc ttccgtcttt tcggtctgtt ccatcagggc ctgcatacgc
     1081 ttttcagcgt cggccagcat ttttgcataa tccattattc taaacctcct aagattttga
     1141 ttttgagttt ttaagcatcc cgcaattccg catcgctctc cagcgacgtc gcacgactct
     1201 ccagtcgtgc tttggcccgt gcactctcca gtacccgctg cgcgctctcc agcgcgtcgg
     1261 cagcctgccg ggcgttgatg tcagtatctt cataagcggg aaaggtgacc gcgcttattt
     1321 caaatacttt cgcaagtttg aggatgtgcc gggtcggcat atccgagtct aaatcggtcc
     1381 agcggtcttc gttgacccgg aaactatagc tcatcccgtc gatgtcgcca cgctggatgg
     1441 ccgaatagag ctgacgggct tccggattgt tatcggtatc gacagacgca ctgaaagcca
     1501 ggccggtgtt gtcgaccatg agcgccagcg tactgttccc gttgttgttg cggctccgtg
     1561 ccaacggaat cttgtgggag tcgtggttga cgatgaaagg tacatcggtc aggtcacagc
     1621 cgtcgaaagc accgggctcg atgacttcat tgaaccaatc gccgatagct gtttgacgac
     1681 tgtaga
//
LOCUS       DAWCYS010000387         1685 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3268_length_1685_cov_5.700000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000387 DAWCYS010000000
VERSION     DAWCYS010000387.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_1685_cov_5.700000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          392 a          478 c          500 g          315 t
ORIGIN      
        1 gccgactact gcatggctta tggttccctc tcgaactaca ccatcgcaac gttcgccgat
       61 attgaagtcg cccagtctga ccagtacaag ttcaaggaag gtatgatctg caaccgcggc
      121 gacgtctatg caggcggcaa cgtcacggtt tacaacggtt tcctccgtat caaacggaaa
      181 gcgacggcct agaaaggggg tgacaggtca tggctgacgc aacactgacg gccgcagagc
      241 tgcagcgcgc tttgcacatc gactccccgg acgagctcga gaacgcgcag agcttgcagg
      301 cggcggctga gatttatctg gagaacgccg gcgtcgaacg ggattacggc aacgcgctct
      361 ataagcaggt ggtggtgaac tacatcgccc ggcttatgga ccagcctgac ctgcttacga
      421 atctgtccga aaccaacggc ttcatgctga acggtttcat ccaccagctg cggctgagtc
      481 agcaggtgag agcggaggcg gatgcgaatg ctgaccaata gctatggcga agcccgtacc
      541 ggcgcgctga accgccgggt gactatcctg tcgtacccgg aaaaggatga cggccagggc
      601 ggccgctacg tcgataagaa gcatcctgta gaaaccgtgg tctgggccta tgtcgctaag
      661 ccccatttca ccgagggcaa cagcggcggc ggtccggcct cacagattac acagggcttc
      721 actatccgca agcgggccgt cgggctgtcc gacgtcgtgc gctatcaagg tacaacgtat
      781 aagatcctgc acatcgatta ctccggtgtg cgtaatctta cgctgacctg tcaggcggtg
      841 atgcataatg gctaaaacct tttgggtcac gacaaatgtc acggccgagg tcaaaaaggc
      901 cctgggggac gtcacaaagt acgataagga cacccagcag cgcctggatg atgtcgtacg
      961 tgatatgacg gaccgcgtct tcaaggaaac ggtatcccgc gtgccgaaga aatccgggaa
     1021 actggccagc agcatcaaac aggaattccg gccgacgccg cacgggctcc agggctatgt
     1081 caaggccatg gaccacatcg cccacatcat cgagttcggc gcggccggcg ctgtcgtggt
     1141 acctgtccgg cggaaggccc tgcatcccgg ggccgccgga tggtttgcgg cccatgctat
     1201 cgtcccgcag cgtacggcgc atccctttat gcagcctgcg atggacgcgg tccgtccgga
     1261 tttggaaaaa gcagtcaagg aggcggtaga ccatgataag taggattcct ttcaatgagg
     1321 tccagcaggg gctgtatcag ctcctgagcc gcggccagac cgttcctgtc tatgacagca
     1381 tcccggacgg ctcagaggcg atgccataca tctggctcgg cgaattccat ggcgctccgg
     1441 ctgaggaaaa taaaacagtc gtgatgcacc aggtatcgca gcagctccac atctggagcg
     1501 accagaaagg caagctcgaa gtaaatggta tcatgaacga tattgtcacg ctgctgacca
     1561 agtaccagct gtccttagac ggcttccgtc aggtcggcgg cgctacggta tcgctatatc
     1621 agacgaccgg agagctctat gagaacggcg ataaggccta tcatggaggc atccaggtcg
     1681 aatat
//
LOCUS       DAWCYS010000388         1680 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3281_length_1680_cov_4.892308, whole genome shotgun sequence.
ACCESSION   DAWCYS010000388 DAWCYS010000000
VERSION     DAWCYS010000388.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_1680_cov_4.892308"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          369 a          479 c          519 g          313 t
ORIGIN      
        1 aatatagccc ccatcgagga gcggtacggg caggagattc aagatgccca ggttgaggct
       61 gaggaaggcc gtgaacatga gcaaattggc aaagcccgat gcagcgacct gaccggccag
      121 ctgagcgacg ccgataggac cggcgacttc ggccttttcc cggcccgtca ccatatcata
      181 gaggccgacg atcatgagct ggcagagctt gcctgtccgt tggacggcca tgcccgccga
      241 ttcaaagaag ccatgagctt ctttggtcgt catgggcatg acgccgagca tggctttctt
      301 cgaggcgctg tctacgtcag gcaccatttc gacgccctgg atctggccgt cccgttcgat
      361 ttccacggta acgacggcgt ggctgtatgg cgcgacggcc tgagaaatat cagaccattt
      421 ggaaatttcc tggttgccga tgcggacgat gcggtcgccg gctgcaaggc ccgactgttc
      481 ggccggtgag ccggcgatgg tcgagccgac gacggcttcc ggcgaaacgg tcaccgtacc
      541 gacgacgaag aacaggcccc acaagaggac gatggccagg acgaagttca tgataccgcc
      601 ggcagcgatg acgatcatgc gcttccagac aggtttgctc aggaaagatt tatcgttcaa
      661 gtcttcctct tccgtcatgc cggcgatgcg gttgaatccg ccaagaggaa tggcccgcag
      721 ggaatattct gtatctccgc ggcgtacact gtacactttg ggaccaaagc cgagtgcgaa
      781 ctcatcgacc tgcatccccg cccacttggc cgtcgcgaaa tggcccagtt cgtggacgaa
      841 gacgatgaca ctgaagacga agatcgtcgc taaaatagta agacccacgg gaaatcccct
      901 ttctacagca aggaagcagc acagcggcgg gcccaggcat cggcctggcg gatagacgcc
      961 agggtgacgt catgcgagac cgtgtgccgt tccatgactg tacggacaat atcaaaaata
     1021 cgcgtaaagg aaatctcttt acgcagaaaa gcatagacgg cgacttcatt ggccccgttg
     1081 aggacacagg gcatggtacc gccgatgcgg ccggcttcat aggccaggcc gatggatggg
     1141 aacttctcgt cgtcaggcgg gaagaattcc agcgtcccca gcgtcttcca gtcgatgggc
     1201 cgggcgccgc ggacggtcat ccgttccgga taggtaaagg cgtactggat gggcaggcgc
     1261 atatcgggca tgcccagctg agccaggaca gaaccgtcga tgaactcgat catggaatgg
     1321 atgatgctct gcggctggat gacgacttcg atcttgtcga agggcatgtg gaagagccag
     1381 tgggcttcga tgacttccag gcccttattg aacatcgtcg ccgaatcgat ggtgatcttg
     1441 ccacccatgt tccacgtcgg atgcttgagg cactgttcga ccgtaatcga cgaaaattcc
     1501 gacgcaggcc ggcggacccc atttccgccg gaagccgtga ccagcagttt ggccacttct
     1561 tccgggcggc gcccctggag gcactggaag atggccccgt gctcgctgtc gacgggcaag
     1621 aagcggacgc cgcgggctgc ggcgtacttc atgaccagta caccggcagc gacgaggggt
//
LOCUS       DAWCYS010000389         1679 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3286_length_1679_cov_4.322044, whole genome shotgun sequence.
ACCESSION   DAWCYS010000389 DAWCYS010000000
VERSION     DAWCYS010000389.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_1679_cov_4.322044"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          445 a          451 c          436 g          347 t
ORIGIN      
        1 cgcggcagat cgaatgcgcc cgcctgaatg tcactaacat gatcacgagc aagcgtaaac
       61 tgcgcctcct cgtcgaaaag ggcatcgtca gcggctggga tgatccccgt atgccgacgg
      121 tttccggcat ccgccgccgc ggctatacgc cggaatcaat ccgcgacttc tgcgaacgca
      181 tcggcgtcgc caaggcagac agcatggtcg atatttccct cctggaatac tgcatccgcg
      241 aagacctgaa gatgaaagca ccgcgcttga tgacggtcat cgacccgatc aaggtcgtcc
      301 tcaccaatta tccagacggc aagacggaaa cgatgacggc cgacaacaat caggaaaatg
      361 aagccatggg cacgcgcgaa atgacgttca gcaagaacct ttatatcgaa cgcaacgtct
      421 tcatggaaga accggtcaag aagttcttcc gcctgtctcc gggcaaggaa gtcagcctca
      481 agtatgcata catcatcaaa tgcgaagaag tcatcaaaga cgaagatggc aacatcgtcg
      541 aactgcactg cacgtatgac ccggaaagca agagcggcag cggtgccaac gccaaccgca
      601 aggtcaaagg gacgttgcac tgggtcaatg ccgaagatgc tgtcgacgtc gaaacccgcg
      661 tctatgacta tctcttgaag gacgacggca gcgaggaaga aggggaaagc aagaatttcc
      721 tcgaccagat caatccccac agcctcgacg tataccacag caaggccgaa aaggcattgg
      781 caacgtatca tgtcggtgat aagttccagt tcctgcgcca ggggtatttc gtcattgatc
      841 ccgatacgac ggcagaccac ctggtcgtca accgcatcgt cggcctccgc gacacctggg
      901 ctaagatgca gaaaaagaaa aaacaataag cgattattaa aaaaggggct gcctttctca
      961 gaatggatta gaatttcatg agaaagggcg gccctttcgt atcttgcgtg tataatggag
     1021 gtaggccatg aaaacccctg gctcacacag tctgttgttc ttctttattt tcctcatcgt
     1081 cggcggtatc ctcggtggca tcgtcggaga ggccctgtat gctgttccgg ccttgaaggg
     1141 tatcctgccg gccctgacgc agcattatga aatcttgaat atccagaata ttcacctcaa
     1201 tctctatctc atggaattgc agtttggcat ccatctggca ccgaatatgc tcagcatcat
     1261 cggcgtcatc ctggcagcca ttctctttcg tcatcttcaa taatttagga gggattatta
     1321 catgctcatc ttagcttctg gttcgcctcg tcgccgcgaa ctgctgacgc agatcggcgt
     1381 cgcctatatg gtcaatccga gtacgtacag tgaagaatca ccgaagaaaa agaatccgga
     1441 aaaatacgtc caggcccagg cgttgggcaa ggcccgcgat gtagctgaaa aattcccggg
     1501 acagtgggtc ctcggcgccg atacgattgt cgccatcgat ggggaaatgc tgggcaagcc
     1561 ccgcaatgaa gccgctgccg tcgagatgct acgggaattg tccggaaaga aacactgcgt
     1621 ctattcgggc atcgctttga tcaatggcca taaggaatac acgaaggtcg tcgaaacga
//
LOCUS       DAWCYS010000390         1669 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3304_length_1669_cov_5.339529, whole genome shotgun sequence.
ACCESSION   DAWCYS010000390 DAWCYS010000000
VERSION     DAWCYS010000390.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3304_length_1669_cov_5.339529"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          382 a          472 c          490 g          325 t
ORIGIN      
        1 atagccatct tatcatagta tagaagggga gggattcttg aggggtttga tcaaagcagc
       61 tatcggttct gtcggcggcg tcctggccga ccagtggaaa gagttttttt actgtgacag
      121 cctgaatgaa aatatcttga tggccaaagg tcagaaacgg accagccgtc atggccgcag
      181 cggcaatacg gacggcgaag acaatatcat ttccaacggc tcgcgcattg ccgtcaacaa
      241 cggccagtgc atgctcatcg tcgaatcggg aaaaatcgtc gaagtctgcg ctgaaccggg
      301 cgaatataca tacgatgcgt cgacggaagc gtctatcttc gacggcgact atgccggtga
      361 cctgggcgaa tcgttccgcc tgctcgtccg ccagatgaag gaacgctttg cctttggcgg
      421 cgatacgggc aaggaccagc gcgtctacta tgtcaacctt aaggaaatca tcggcaataa
      481 atacggtacg cccagtgaag tgcccttcaa gtcttatgac gatacgacgg gactggccct
      541 gctcatgcgc ctgcgctgct tcggtgaata cagctaccgc atcgtcaatc ccatcctgtt
      601 ctatacgaac gtggccggca atgacgtcga cgtctacgaa cggagccgcc tggacagtca
      661 gctcaaatcg gaactcttga cggccctgca gccggccttt gccaggattt cagccttgca
      721 catcgactat tcccagctgc ccggccatac gccggaactg gctcaggccc tgaatgaagt
      781 cctgtcgccg aaatggcgcg acctgcgggg catcgaaatc gtatctatcg gcatcaacag
      841 tgtcaaagcc aatgaagaag acgaagaaaa gattcagaaa gctcagatgg gcttgaccat
      901 gtctcggccc gatatcggcc tggggaccct cgtcgatgcg acggccgatt ccatgcgcac
      961 ggctgcagcc aatgaaggcg gcatgggtgc tgccatgggc tatatgggca tgaatatggc
     1021 caatcaggcc ggcgccggcc tggcccagga actggcagcc cgccaggcgg cccaggcgcc
     1081 ttcgccgatg ccgcaggctg cgccggccgg cagctggact tgtgcctgcg gtcaggccgg
     1141 caacacgggg aacttctgca cggcctgcgg ccgtcccaaa ccgcaggccg ccgctgcctg
     1201 gacctgcccg aaatgcggcc aggccgggaa tacgggcaac ttctgcagca attgcggcgc
     1261 gccgcggccc caggttccgg ccaactggac ctgcccgtcc tgcaggactg tcaatacggg
     1321 gaatttctgt atgaactgcg gtgcaaagaa gccggaaagg taaggtgagg gtcatggcgg
     1381 atacatcggt cagctatcaa tgcccgaact gtggcgcacc gctgcaattc aagccgaaag
     1441 acaagaccgt cacttgtgac tattgcgata cgaagttcga cgtccagacc ctggaagcca
     1501 tctatgccca gaaagaagcg cgggctgccg aagctgatgc ggcccgcaag gtccgttgga
     1561 agacgtaagc ggcatgacag tatttttttt tttatgattc ggctaatata gagatctaca
     1621 cgtgttcgtc gtgtggcgcc gaaatcgtct gcgatgagaa taccatggc
//
LOCUS       DAWCYS010000391         1665 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3313_length_1665_cov_5.484472, whole genome shotgun sequence.
ACCESSION   DAWCYS010000391 DAWCYS010000000
VERSION     DAWCYS010000391.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_1665_cov_5.484472"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          379 a          540 c          454 g          292 t
ORIGIN      
        1 tattgccggg cgcatcggcg aaatggggaa tatgaagccc ctgcccatga agactcacgt
       61 ccagctcttc ctcgaccgct ggcaggatgc gcgccatttc gagctggccg acaagcagcg
      121 cgaagccgta gagaaatccc tgcagtccgg catgaccgtc atcaccggcg gccctgggac
      181 gggcaagacg actgtcgtcc agaccatcat ccgcctggcc gaacaggaag gcctgcgcat
      241 cctgctttgt gcaccgacgg gacgggcggc caagcgcctg gccgaaacga cgcagcgcaa
      301 ggccaagacc atccaccgcc tgctcgttcc cgacggccac gtcggggcca tgcaggtctt
      361 tgaatacaac gaaacgaaga tgctgccggc cgacctggtc atcgtcgatg aagtatccat
      421 gctcgacatg gaaatgatgt accatctcct cagcgctctg aagccgcagt gccgctgcat
      481 cctcgtcggc gacgccgacc agctgccatc ggtcggggcc ggcgccgtcc tgcacgatat
      541 catcgcgtcc ggtcaggtcc cggtcgtccg cctggatacg atttttcgtc agaaagaagg
      601 gggacgcatc gtcaccaatg cccatctcat caacagcggc cgcctgcccg tcgtcaatga
      661 agacacggaa ttccgctttg tcgaaatcga caacgaagcc gacggcgccg aaaagataag
      721 cgccctctat aacagcgaat tattggaaac gggcgataaa ttcgccgtcc aggtcttgtc
      781 gccgatgtat aagaacccct gtggcgtaga taacctcaat cagctcatcc aggaacggtt
      841 caacccgccg gccgaaggga aggccgaact gaaagggaag aacgtcgtct tccgcgtcgg
      901 cgataaggtc atgcagaagc acaacgacta tgaaaaaggc gtcttcaacg gcgatatggg
      961 tgaaatcttt gccatccaga aggacatggt ctatgtccgc tatcccgaac aggatgtcaa
     1021 atacgaaggc caggaagtcg atgaaatcac cctggcctat gccattaccg tccacaagag
     1081 ccagggcagt gaataccaca cggtcatcat ggtcctggtc aacagccacg ccatcatgct
     1141 ccagcgcaac ctcttctata cggctgtcac tcgggccaag cgcaaagtca tcctcgtcgg
     1201 caccaagcgg gccgtccaga cagccgtcca gaaccagcgc acgagccgcc gcttcacact
     1261 gctcatccca cgcctgcaag gagaactcat cgtctaggga gggaagacca tgtccctgat
     1321 cacgcagctt ttcaacctct tatatccgcc gcgctgtccg tcctgcggtg ccatggtagc
     1381 ccatgatggc gactggtgcc cggcctgtct ggctgccatc tggcatcccc ggcgcatcaa
     1441 caagacgccg gctgtgaggc acctcagcgc ctgttactgc ctgaccgatt accgcggcgc
     1501 catgcgccat gtcctgcacg acatcaaata taacggcaag ccagggaagt gccaggcctg
     1561 ccggatcctg ctcgactgct tcccctggcc ggaacggtgc gaccgcatcg acgtcgtcgt
     1621 ccccgagccg atggcaccgg aaaaattaca tcccagcggc ttcaa
//
LOCUS       DAWCYS010000392         1657 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3328_length_1657_cov_7.177903, whole genome shotgun sequence.
ACCESSION   DAWCYS010000392 DAWCYS010000000
VERSION     DAWCYS010000392.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_1657_cov_7.177903"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          283 a          438 c          541 g          395 t
ORIGIN      
        1 cttaaacagc aaccttctca agaatctccc gcttctataa gcgggagagg ttcaattctt
       61 tccgttcttt ccgtgtttct tttcaaaagc aatcatgtgt gcttcttggt cggtatggcc
      121 ttcggcttct gcctgggcga tgagggcgac catttcttca taggcttcag gaatgacttt
      181 ggtcaggtga gtgacggtgt cgtcccagtt ttccaagagg atgtggccca gattgctgtc
      241 cgtgtagtgg acgtgttttt ccagcatggc tttgacagcc gctttttcgt cgtcgtcggt
      301 gagcggttcc agtttgacca ggtcggtgtt gcatttattc gggtccaagt cgtagacgta
      361 ggcgatgccg ccggacatgc cggctgcaaa gttgcgccct gtctggccga ggatgaggac
      421 ccggccgccg gtcatgtatt cgcagccgtg gttgccgacg ccttcgacga cggcactgat
      481 gccgctgttg cggacgcaga agcgttcacc ggctgtgccg ttgatgtagg cttcgccgct
      541 ggtggcaccg tagaaggcga cgttaccgat gatgatgtta tcttcggcag ggaataccga
      601 ttcccgcggc ggcgatacga tgatcttgcc gcccgacagg cctttgccca ggtagtcgtt
      661 ggcatcgcct tccagggtca gcgtcaggcc acaagggatg aaggcgccga agctctggcc
      721 ggcagagccg acgaaggaca agatgatcgt gtcttcggcc aggccttttt cgccgtagcg
      781 cttggtgatt tcactgccca ggatggtccc ggtgacgcgg tcggtattct tgatgcgcag
      841 gcgggcccgg atatgtttct gttcttccag ggccggccgg cacatgcgga tcagtttgga
      901 catatccaag gtcgtattga tcttgtggtt ctggggcgtc gtgaaatgct ggccgaccga
      961 cgcatccgtg tacggacggt acaggaggtt cttcaagttg accgtcttgg ctttccagtt
     1021 gccgaagtga tcgcgctgtt tcaggcagtc atagcggccg accatttcgt cgatggtatg
     1081 gaagcccagg cgggccatga tttcccgcag gtcctgtgcg acgaagcgca tgaagttgat
     1141 gatatattcc ggtttgccct tgaagcgttt gcgcagtttt tcatcctgcg tgcagacgcc
     1201 gtacgggcag gtgttgaggt ggcagacgcg cagcatatgg cagccgatgg ccaggagcgg
     1261 tgccgtaccg aagccgaaga cttcagcgcc gaagagggcg gcaatggcga cgtcgcggcc
     1321 tgtgagcagt ttgccgtcga cttccagggt gacgcggtcg cgcaggcggt tgaggagcag
     1381 cgtctgctgg acttcggaca ggccaatttc ccagggcagg ccggcatggc gcaggctcgt
     1441 ccgcggcgaa gcgccggtcc cgccgtcgta accgctgaca acgatatcgt cggccttggc
     1501 tttgacgaca ccggcggcaa tcgtgccgat gccggctccg gacgtcagtt tgacgccaat
     1561 cgtagccttg cggttggcat tcttgaggtc aaagatgagt tcggccaggt cttcgatgga
     1621 ggtcttatcg tgatgcggcg gaggcgtgat gagggcg
//
LOCUS       DAWCYS010000393         1650 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3344_length_1650_cov_4.639498, whole genome shotgun sequence.
ACCESSION   DAWCYS010000393 DAWCYS010000000
VERSION     DAWCYS010000393.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3344_length_1650_cov_4.639498"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          317 a          459 c          442 g          432 t
ORIGIN      
        1 tacgatcttg agctttcccg cttccttctc gatacgcttg atgcagttgg tgaccaggtc
       61 gctcaggtag atgtcccggc cgaagcggtt catgacggcc tggttgccgg tgtagatgct
      121 ggtcatgagc tggtcattcc gatgcgactt gtatttcgtt aggatgttct gaaagaattt
      181 cagcaccgga tgtcacctcc tggccccggt tccggtaaaa ccgatttctg atgttcatga
      241 tattccgatt tgaagcgccc atacgcggca taggcgatga tgaagccgag ggccccatcg
      301 atacggttct tcgactggcc gtacaccttg accggcatca tcatgccgag gttgttgacc
      361 ttaaatgctg tgttggccaa gcaccatttg tcaatcggat tgtcgttgta gttaagcacc
      421 ttgcggcgca aatcgctttc cagcgctgac atggggttcg ataaagacag atagtccatg
      481 atgattcgtt cgagaacttc atcgccgaag tattcgccga tgtatttctt gaagtccttg
      541 gcgtgccaat tatcgtaccc gattttgaag gggatgacgt gatagtcgtc atacagcttc
      601 aagaaccagt cggcgaccat cttcgcgtcg acttccgtgc ctgggcaaat cgtgaccaat
      661 ctttctctgg cccattcccg gtaatctttc tgttccggat tcagggcatc gccgttctcg
      721 gtacccaggg cctcggcctt gatttccggc acgaaataca tcgacaacgt ctttttctgc
      781 cgcgtccacg ggtcgacgaa gagcgccttg gcgctgcaaa ggtccggcgt ttccgcaaag
      841 tcgagggaac cgatgtaata ctggccgtcg agttcctccg gcgcgaaggt ccgcgggttc
      901 tcgatagtag ccgactccag ccaggctgca gccgtgttct gtttgatatt gaaatccttg
      961 gccagcacga aggcccgcgt cttgcggttc gtccgggctt catcgaccat ctgacgcatg
     1021 aaagaccatt tcttaatggt acccatgccc ggattggctt tataccagga ctgctcatcc
     1081 tggaaaatct cttcctccgt atcctgctga tacaggaaga tgagccagcg cggcatctca
     1141 gcctctcccg tcagcacctg tttggccagt gccatacgtt catcgaggta accgccgtcg
     1201 gtaaagcctt ccgtcgtgat ttcgatgtaa agcggctcat cctgtgtgga cagggcctga
     1261 cggataggca tgaccagcgt gttgtcctgc atttcatgga cttcgtcgac gacgccgatg
     1321 gagatgttct tcccttcctt ggcgctctgc cgtgccgaca gcttcttgat ggtccctttg
     1381 ttctgccggc tgaatttccc gcgggcgtgc ttctgcttct gattgcccca gaagatgccc
     1441 ttctggttct tatgggtgca tcgagccagt ttcggcgatt cctcgcgcat gtcattagcg
     1501 cactggaaaa ggatgtcgga ctggtcaaag tcgttggatc catacaagat gttaccgccc
     1561 atcggaccgc acacccattc agcaagcgat gtggccgctg ccagcgggct gttgtgcgta
     1621 gccgtcatcc ggagaccagc tagatacaga
//
LOCUS       DAWCYS010000394         1635 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3370_length_1635_cov_4.510759, whole genome shotgun sequence.
ACCESSION   DAWCYS010000394 DAWCYS010000000
VERSION     DAWCYS010000394.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3370_length_1635_cov_4.510759"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          372 a          507 c          461 g          295 t
ORIGIN      
        1 ccggggcctc ctgctccggg agggcggggc cgtgtcggtc aggatcaatg acggagacca
       61 tttctgcatc cagacggcag cacctggttt cgacctccag cgcgtctggg accaggcggc
      121 ccaggtcgac gatgccctgg aaagccacct ggatttcgcc ttccgcgacg atttcggtta
      181 cctcacggcc agtccgtccc tgaccggtac gggcctgacc gtcggcgtga ccatccacgt
      241 cccggccctg atcctcatga agcggttcaa ccgcatcgtc cagggcatta ctaaattcgg
      301 cttcaccgta tccgggatgt acggcgaacg caatgaatgt atcggcaaca tcttccagat
      361 caccaatcag attaccctgg gagtgaccga aacagatatc ctcgaccagc tgcgcaaaat
      421 cgtcacccag attgtccagg aagaacagaa ttgccgcagc ctggtctggg accacaatga
      481 aaatacatta aaagataaat ggctgcgtac ttacggcacc ctcagccggg catggctcct
      541 ggccgaccag gaatccctgg ccctggccag cgacctgcgt ttcggcatcg acatgggggt
      601 catccccgaa ggccctctgg cttatgaagc catcatgacc gtcgtcgaac cggcttatct
      661 gcagctcaag gccgggaccg aactgaacgg ggaagagctg gaacaccagc gggctgaagc
      721 cgtgcagcaa gtactcattg cttaccggag gaagtgattt cccatgatga acaatcgtct
      781 caccaatgac gctaaagccg tcctgcagta tgcgcaggaa gtggcacaga aattccacca
      841 tgattacgta gggaccgaac atatcctgct cggtctcgtt ctcaatacag acggcatcgc
      901 cggcctgctc ctcaaccagc tgggcctgac gccggaaaac gttacccgtg ccatcgaaca
      961 gcatgtcggc tggggccagg attcgccgac gaagctgcgc ctgacgccgc ggacgaagcg
     1021 ggctatggaa ctggccgtcc gtgaagccaa ccggctggaa cagaactatg tcggcacaga
     1081 acatatgctg gccggcatcc tcgaagaagg gagcggcatg gccgtccaga tcctggaaga
     1141 tatggacatc gactcggacc tggtctccca gcgcctgagc gagcttatga acgatccggc
     1201 cgtcgaaggc ggcgatgccg taccgtccaa gaatggcagc gacttgtccc agttcggccg
     1261 cgaccttaat gaatgggcca aacaggggaa aatcgacccg gtcatcggca gggaaaagga
     1321 aatcagccgc gtcatccaga tcctcagccg ccggaccaag aacaatccgg tcctcatcgg
     1381 cgcccctggc gtcggcaaga cggccatcgc cgaaggtctg gcccagcgca tcatcactgg
     1441 caatgtgccc gacagcctgc tggacaagcg cattttctcc ctcgatttgg ccagtatcgt
     1501 tgccggtacc aagtaccgtg gtgaatttga agaacggatc aagcgcatcc tcgacacgat
     1561 cgaagaggat gactcgctca tcctcttgct cgacgaaatc cctcagctca tcggagccgg
     1621 tgctgtcgcc ggcgc
//
LOCUS       DAWCYS010000395         1632 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3381_length_1632_cov_4.546607, whole genome shotgun sequence.
ACCESSION   DAWCYS010000395 DAWCYS010000000
VERSION     DAWCYS010000395.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3381_length_1632_cov_4.546607"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          440 c          436 g          304 t
ORIGIN      
        1 ggtacaggac agctacgtcc aggaaatcca gggatggggc gaaacagccc gccaggaact
       61 gggcggaaaa ttagacgaaa ccgtagcagc cgcttgtacg acgcgtaact acctggaaca
      121 aaaagtgccg ggctttacga aaatgctgaa cctcactggc gctggcaacc acattgccat
      181 gatccgcgcc atggccggga tggcgtctct tgtgggcgaa gacccgggac acagtggcaa
      241 gggcagcggc ggaggacgcc gccgctcgtt atatgatcag acagattttt caaaatatta
      301 ggaggctaaa ctatggctta cggtaacacc gcattgactt tttcggatct gcgcaagcgc
      361 atggatcccg atggcaagat tgcctggatc atggaaatca tggcccagag caacccgatt
      421 atgcagcaca tcccctggat ggaaggcaac ctgccgacag gcaatcagac gacactgcgc
      481 acgtcgtatc ctcatcccca gctccgccgt atcaaccgcg gcattacacc ggggaaatcg
      541 acgacccgtc agattgtcga tacctgctgc ctcatggaag gcatgagcca ggtagacgtc
      601 cgtatcgtca acttggcacc ggacaaagaa gcaacccgac gttcggaaga cggcgccttc
      661 gtcgaaggat tcacccagga cctggcaaaa tatatgttct acggcgacac agaaaaggac
      721 cccgacgaat tcaatggcct gggcattcgc ttcaacacct tcacgggtga taaaggaacc
      781 tatggtttcc agactattaa cgccggcggc acaacggaag ataagcagac gtcgatgtac
      841 attgtcgact ggggcgaaaa tgccgtcacc ggcatttatc caaaaggctc ccaggctgga
      901 ctcaaaatgg aagacaaagg cgagcatatc gtggaagata gcgaaggggg caagtacaat
      961 gccctcgata cctggttttc ctgggacgct gggctggccg tccagaatct ccgttaagta
     1021 gcggctagcc gcaacgtcga tgttgccaca gccccgacag ggattacggc agccgaccgg
     1081 aagaaactcg tcgaaaatat catcgtcgca aagaaccgta tcgtcaaccc gaaacgcccg
     1141 attctctacg tgtcggacaa agtctataca attctcgaat tatatttgaa cgacaaaaac
     1201 aacatctacg tcacccagag cgaagccttg aacggaatcc cgaaactcta tgtacagggc
     1261 ctggaagtgt ccaaatgtga cgccctgagc gataccgaac cggttattac agaataggag
     1321 gaccctatgg tttacgatgg cgaaaatacc ttttactgga agaaagcact gaacgggacg
     1381 acgacaggca cgtcggacgt cgtccagacc ggcaaagggg atgcgggcaa tccgcttatc
     1441 ctctacgtca cggctcccgg cgctacggac gatttgaccg tcgaattaca gacggctgtg
     1501 gacgaagcct ttacaaaagc cgttaccctg ggaacgtata ccctgaaaaa agacgacggt
     1561 ttgaaaacaa aagttcccta tggggacttg ggcttcatgc gtatcaaata cactggcgca
     1621 tcggccctga ct
//
LOCUS       DAWCYS010000396         1621 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3405_length_1621_cov_3.802043, whole genome shotgun sequence.
ACCESSION   DAWCYS010000396 DAWCYS010000000
VERSION     DAWCYS010000396.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_1621_cov_3.802043"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          446 a          405 c          362 g          408 t
ORIGIN      
        1 tcctgcagct ccccggcttc atccagggca ttcctggcct ggcgataggc tttcgccgcc
       61 tggtcatacg acgtaccgcc gacgcggtcc acacgctgcc ttccctgacc atcttccgtc
      121 gggacttcaa tcgtataata ccatttcttg cctcgttttc gtaaatacat atgcggcacc
      181 tcctttgttt actattgtac tatgagaaaa gacaagatgc aacaaacgtt cctgttgaat
      241 ttgccgattt aaggaaaata aaacaagttt cccatgtgat tactcacaag atgataaaaa
      301 acgtctcata aggccattta tgaaaattag gcggattttt ttgcaaataa agaaactatg
      361 ttcgacgaaa cgattatccc tttagcgaac atagtttcta tttgctttat tgggccgata
      421 tggtacaata aaaatagaga attagcaaat tgttggattc ggtagaagca aagcacaatt
      481 tttatggaat atttacgctg tttatacatt cctttgttat actgaaaaga ctagcacaga
      541 aaaggaatga tatcatgaag aaactgactt atgaaaatat tgcccatgcc aaagagctga
      601 atgtttattt agaacaggga aaccgggtcc tcgatgctct ggggtatacg gaccattcgc
      661 aaaagcatac ggctaaagtg gccgtcacgg ccggcaaaat cctgaagaag ctcggctatg
      721 acgcccacat ggtggaactt gtgcgcattg ccggttatat gcacgatttg gggaacagca
      781 tcaaccgcgt agaccatgcc catacggggg ccctcatggc gttccatttc ctgcgcgact
      841 ggggcggacc accagaagat attgctgcca tcatctcggc cattggccag cacgaggaac
      901 ggaccggcat cgcctttgac cccctgtctg ccgcccttat cctggccgac aagagcgatg
      961 tccgccggga ccgcgtccgc aacaccgtac cggaaacctt cgacaaacac gaccgcgtca
     1021 attatgctgc cctgtcgtcg acgctccacg tcgacccgcg caagaaagtc attaccttga
     1081 acatcgagct cgatgaatcg atttgctcca tgattgatta ttttgaaatc ttttcccagc
     1141 gtatgctcat gtgcaagcgg gcagccgaag ttctgggcct gcgcttcaaa cttgtagcca
     1201 atggcaataa aattgcatga cagcttctaa ctacatataa ttaccataaa aagtacatat
     1261 ttcgcagctt atcgaaaaga aggcgacacc taatttttaa catttaggca tgttgccttt
     1321 tttttccatg atacacttat aatatagttt aaaacatgtt gtagagaaag gaatcatgca
     1381 tatgctacgt tatggaaaag gaacacttct cactacggtg atggccggac tgctggccct
     1441 gtccctgctg acggcaggct gcggcagcac gacaccgact aagagtgccg ctgtgtcggt
     1501 caaggccatg aaagtcctcc agcaggatac ggctgtgagc cacgactatt cgggtcagat
     1561 catatcgacc aacgccgtca ccatcaagcc ccgcgtttcc ggagaaatcg tagagaaata
     1621 c
//
LOCUS       DAWCYS010000397         1613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3427_length_1613_cov_4.388960, whole genome shotgun sequence.
ACCESSION   DAWCYS010000397 DAWCYS010000000
VERSION     DAWCYS010000397.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3427_length_1613_cov_4.388960"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          314 a          483 c          417 g          399 t
ORIGIN      
        1 cccattcttc aaatcctgct gccgccaata cttcctgctc attggagcct tccacccagt
       61 agacagaccg gaacggcatg tgctggtttc ccagttcgtc ggcatcagct ttatcgtttt
      121 cttcgacgag ccagcaaacg gtaaagtatg tgctgtagcc attattactg cggtagacat
      181 cctgcacgct ctgtgggcag ttctccaggc caaatttgcc gacaatctgg gctgctgtca
      241 tgcggacctt gcgggcaaag gtattgacga taccctgggc attggtgccg agagcatagg
      301 tgccaatggt atacggcaca aaggttatgc cgcgtccggc ggcaaatatg cccataggcg
      361 cctgccccat gggcaattcc atgtataccg tatggacggc attgtagaaa ttgctccgtg
      421 ccaggacata ctctgtaatg tcacagcgct gatctaacac gcgcttgacc atgacgttat
      481 cattgagcgt catgtcggcc agggtatagc ggaaccactt ccgcgacggc ggcgtcaggc
      541 ccgactggat ccccgccgca aaaatactgc ggcagtcgcc tggcgtcgtg ttgtagatat
      601 tgtcgtcgtg caggttcggc ttgcctggct ggtcatcgtc gaactggcca tcgtagggaa
      661 gttcattgtc ccgtatgtcc ttccatatat ccagccagcg ctggcggttc cgaaagagtg
      721 cggcatagcg ctgtacgagt tttgatttct gctggaccgt gttggtcttc ttgaatttat
      781 ggctgcccgt cggcgagcgg gccaattctg tttcaataac cggtctcata gcatcaccct
      841 aatgtattct tgccattggc gctccctaaa atggagtcca ggccggaccg gaagtcccgc
      901 aactgcgttg ccgcaaagcc gcgtttcttc ttcgcatttt tcgtggcatc gtccgtcgta
      961 ttgctcgtat catcgccgac gttgacggtc gttgctacgg ggtctgcctg cgggaccgtc
     1021 ggggcgctgc tgctgccccc gccaaatcct aaaataccgc caatggcttt ccctactttt
     1081 ccacacatag tgttacctcc tcttgaacaa atcgtatttc gtattcgccg tccctatatc
     1141 cgcatccctg cggagtacag gaaaggcaaa ggtcagagcc agagcatctg cttcgttcgg
     1201 agatggaagc cctcgccgct tcatgtcttc ttttttctcc agctgtattt cgcctttagc
     1261 attgacaaag gcttccggcc cgatgaggtc gtcatggatg acgtccccgt cttcgagtac
     1321 cccgccatcc cgcagccagt cccggagggc gccccacatt tcggcccgtt tattggcata
     1381 gccccgcttt ccggacgcac tgccaaaagc cacgagccgc cactggcgcc ccatggtcag
     1441 gccgaaagag tacacgcccg ttccgtaacc ctggtcaatg aatacggcct gggcctggta
     1501 ctcatcttca aagacagcaa tcttcccggc aatgacgccg tcgatgtcgt ttttcgcgta
     1561 cgacgccatc ttgcggctgt acagtccctg ccgcacatag atgacggacg caa
//
LOCUS       DAWCYS010000398         1599 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3456_length_1599_cov_5.159326, whole genome shotgun sequence.
ACCESSION   DAWCYS010000398 DAWCYS010000000
VERSION     DAWCYS010000398.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3456_length_1599_cov_5.159326"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          344 a          424 c          457 g          374 t
ORIGIN      
        1 agtttgagga ggcccgaaat ctgacggcgt gccttttcgg cagcgtattc ggtcatcatt
       61 tcggccccat tcgacagggc caggagggtc aggctcgatt ctggcttgcc agccaggacg
      121 gacgcagcga ctgccgtcgc cgtcagggta tcggcattgg cctgatggtc ctttacgagc
      181 cccatgatgc cgttcttaat gtagcggcgt gcaatatata aggtaaataa tcggcgaaac
      241 aagaggatcg acccgtagaa ttccgggttg accttgcgca ggatgttcat ggcgatgaag
      301 ccgccgacgg agatgatggc ttcccggcgg tacgtagcaa catccatgtc gtcgtctttc
      361 tgttccttct tgcggccata tttttcttca aaggcacgga tgaacaggtt cagtgcccgc
      421 aggtccagct gtcccacata gtagacggtg accttctttt cttcgacgac agccgagacg
      481 atgccgcgga cctggcggag cttgacgctc aggacagggc gcatgcggct ggggatctcg
      541 ctgtgcagga agatggtatt gcgcatgtac atgacttaac gccccctcaa caacgacagg
      601 gcccaccaca gcatctgtgg tcctgacggg cgctgtccca tgaatacgac tttgcgcaag
      661 ccgcgaaggg tcaggagcaa ggcagcgact gtgcgcaagt caaaaaaacg actggattca
      721 ttgcggatga tactgctgaa aaaagccccc gtatcgcgga ccgctttgag atacgtcgtc
      781 tcttcctttt tctggccttc ctgtttttct gcatcgcaga cggcactgac gatgccttcg
      841 acgacattcg ggaagaggcg gaagcggaag aagttttcca gccggtccaa ggtcccttcg
      901 ctgtgggcga agaccaggat gctgcccgtg acgacgctga cctggatgaa ctggatattg
      961 ggcagggtgc tgactttttc ttcgatcaag cgggccagtt ccgcattgcc gacgagggcc
     1021 gctgcccgat agcggcgacg gcctttcaag acggatacgc aggcgaaacg gggcacttcc
     1081 ggtactccct ttgccgcctg gacctgaggc cggaaggcac cggcgggcgc agctgcggca
     1141 gccgctttgc ccttcttatt gtggaacaag tcgtatacgg ctttgccgat cgacgtgccc
     1201 accataaaac ctgaggcata agacataatt accttctcct ttcaacatgg gtcatgatat
     1261 agttctctac ccggacgagt tcccgattgg cccgcaggac ctgcggccgg taggtgatca
     1321 ggacggaacc cgtgaggaca ttgacatcga ccttgtcgat ttctttataa ctggcaatgt
     1381 aggagtagac cttgcggccc aattcggcat tgccaatgag ttttttactg tgcaggcgga
     1441 tacggcctgg taagtaagac gaaatagaaa cggctctcat gatgaggtcc catttcaacg
     1501 tcggaatgat catacaagca gtccttcctg gtgttgttcc tcttaaaaag aggctgtcgc
     1561 attaagacag cctctttttg agttttaagt ttgtagttt
//
LOCUS       DAWCYS010000399         1591 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3477_length_1591_cov_2.591146, whole genome shotgun sequence.
ACCESSION   DAWCYS010000399 DAWCYS010000000
VERSION     DAWCYS010000399.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3477_length_1591_cov_2.591146"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          295 a          518 c          472 g          306 t
ORIGIN      
        1 ggaaggggtc ctgccgttta cacccaatct ccacgatgaa gaggcccggg cttttgaagc
       61 ggcccggctg gtcgtcttca tcggcgccgc aggcattgcc gtccgcacta ttgcgccctt
      121 tatccgccat aaagacctgg acccggccgt cctggtcatc gacgagaagg gcgcgttcgt
      181 cctccccatc ctgtgcggtc atatcggcgg cgccaatgcc atcgcccgcc gtctggctgc
      241 agaactggaa ggccaggccg tcgtgacgac ggcgaccgat gtcaacgcct tgttcgccgt
      301 cgatgaatgg gcggcccgcc agggcctgcg cctgtcgtcc ctgaacgatg ccaaggcctt
      361 tgccgctgcc atactggaaa agggcgctgc cggcgtgtac agcgatttcc ccctgcaggg
      421 gccgctgccg ccgggcctgg tctatgcctt ccatggcgac gccggcctgg tgatcacgac
      481 caaagcccac gtgcccgtgt tccgcacgac tgtcgtcgcc cggccggtca tcgtccacat
      541 cggcatcggc tgccgcaagg gtacgccggc caaagacatc gcttattggg tcaatgccgt
      601 cctacagcgg gccaacttgt cgcccgacgc cattgccgac gtcagctcca tcgacgtcaa
      661 gcaggatgaa gccggtctct tggcctttgc caaggcccag caatggccgc tgcacttctt
      721 tacggccgat gaactcaatg ccgtgccagg cgactttacg gcgtccgatt tcgtcaaaca
      781 gactgtcggc acggataacg tctgcgaacg ggctgccgtc ctggccagcc atcaagggac
      841 cctcatcgtc accaaacaga gccgccgcgg catcaccgta gccgtagcct gcgaacgcta
      901 ccaggtccgg tttgcatgtt gaaaggagaa aggttatgaa acatcgtatt tatgtcgtcg
      961 gcatcggacc ggggtccctg cagggcatga ccctgcaggc ccgggaaatc ctcgatggct
     1021 gcgactgcat cgtcggttac gacacctata ttgatttgat acgtcattat tttcctgata
     1081 aagaattcgt cacgacgggc atgaccaaag aagtcgaacg ctgccgcatg gtccgggacc
     1141 gggccaagca ggggcagacc gtggccatcg tgtccagcgg cgatgccggg atttacggca
     1201 tggccggcat catgcacgaa gtggctgccg atgacgacgt cgacatcgtc gtcattcccg
     1261 gcgtgaccgc tgccagcgcc ggtgcggccc tgctcggtgc gccgctggtc gccgatacgt
     1321 gcctcatttc cctcagcgat ttgctgacgc cgtgggaaaa aatcgccaac cgcctcgact
     1381 gtgccgctgc cggcgacttc gtcatctgcc tgtacaatcc ggcctgcaag aagcgggcag
     1441 actatctgga aaaggcctgt gacatcatct tgcgccatca gccagccgat acgccggccg
     1501 gcatcgtccg caatatcggc cgcgaaggcc aggaagtgac ggtcctgacc ctgggcgaac
     1561 tgcgcaagat gcgcctggat atgttctgta c
//
LOCUS       DAWCYS010000400         1590 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3478_length_1590_cov_5.000000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000400 DAWCYS010000000
VERSION     DAWCYS010000400.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_1590_cov_5.000000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          422 a          445 c          394 g          329 t
ORIGIN      
        1 aacctcaatc cgcagatcga taaagtcgta gaaaacgatt acaccaaaga agaaatgaag
       61 atggtcaacg aaacgcacaa gatgctccac gatgactcca tcgccattac ggctacgacg
      121 gtccgcgtcc cggtcttccg cagccatgcc gaatcggtct atgtcgaaac ggccgatgaa
      181 ctgacagttg atgccgtccg caaagccatc gatgctttcc cgggtgccca ggtccaggat
      241 aatccggctg aaatggaata tcctatgcct ttgttcacgt cggaaaaata tgactgctac
      301 gtcggccgta tccgcaagga tctttacaac ccgaaagcca tcaacctctg ggtatcgggt
      361 gaccagatcc gcaaaggcgc tgcgctcaat gccctgcaga ttgccgaata catgctggcc
      421 cacgacatga tccactagta cgaagagggg agagaatcct ttatgttaac gtttcccaat
      481 gttattgtcg ctatgattac gccgttccat caggatggct ccgtcaatta cgaagctgcc
      541 gtcgaactgg ctaagaaata tgctgacgaa ggatcggcca tcctcgtcgg tgctactaca
      601 ggtgaagggg ctgtcatgac ggaagaagaa aaactcaagc tcttcaaagt cacagccgat
      661 gccctgaaag gccagaccct ggtcatgggc aatgtcggga ccaacaatac ggctcagacc
      721 atcgccatga ttcagaaagc gaaagatacg ggcatcgatg ccctgctgtg catcgtcccg
      781 tattacaata aaccgaacca ggaaggctgt tacgcccact ttgcagctat cgccaagtcg
      841 acggacatgc ctatcttcat ttacaacgta ccgggccgta caggcggcaa aatcgaaccg
      901 cagacggtct gccagctggc tcatgacttc ccgaacatca tcggtctcaa agatgccagc
      961 ggcgacctgg actacgccgg ttacgtcgca gccaatgcac cggaagggtt ccatctctac
     1021 agcggtgatg acaacctgac cctgcctatc gtcgctgtcg gcggcgaagg cgtcatttcc
     1081 gtcgctgctc acgtcatcgg caaggaaatg aacgaaatga tccaggccca caaagacggc
     1141 gacgtcaaga aggcagcggc cctccatcag aagtacctgc ccttcatgaa aggctgcttc
     1201 tgcacggtca gcccggtccc ggtcaaaact atgctcaaca tgctcggctt cccggccggc
     1261 ccgttccgcc tgcccctggt agacgccaca ccggaaataa aggccaagtg cgaacaatac
     1321 ctgaaagaca tcggcagatt ataagtgctg ataaacggaa acctctccga cctgtactca
     1381 agcctccctt ataggggagg tggcccagtg ggccggagag atttctataa acttggtttt
     1441 atattaaaaa aagccttgac atatccccga tttcttgtta ttatatatga gtcgtcagga
     1501 acgacaagat atgactcggt agctcagctg gatagagtga ctgactacga atcagtaggt
     1561 ctagggttcg aatccctacc gggtcaccat
//
LOCUS       DAWCYS010000401         1587 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3480_length_1587_cov_3.242820, whole genome shotgun sequence.
ACCESSION   DAWCYS010000401 DAWCYS010000000
VERSION     DAWCYS010000401.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3480_length_1587_cov_3.242820"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          343 a          495 c          410 g          339 t
ORIGIN      
        1 tggccgtctt cgtccatcac agcttccgcg gcaggggcga cctgcgggac cgggaactgg
       61 tagaacagaa tatccgccag cagacggcga gcgacgtctg ccagaccagt tatgcccgct
      121 accgcgtcat cgcctgtgac ggtaccgtac gctatgcaga acacttcagc cgcaaagtca
      181 ccctgcccgg cgtgggtgag gtctattacg tctttatcgc cgattacgac cgcaaatacc
      241 tgaccagcga catcgacaac atgaccggcc tgcccgggaa ccggcgcttc ctccagtatg
      301 tccgggacct catggaatcc ggtcagtccc tgtcgggata ctgcctggtc tatttcaacg
      361 tcatcaactt caagctcgtc aatatccgct atggcgtcgc cgtcggcgat gactgcctga
      421 aaggcctggc caccatgatt cgggaaacct tcccgcaggg cttcttgtcg cgtcgccgca
      481 atgaccactt ccagctgctc acgccggaag ggtctgtgcg ggaaaagctg gaaacacttc
      541 atgactccat ctgccaggtc tatcgcaagt accagctgga aatcaaggcc ggcgtctacc
      601 attttcctga aaatcaggtc gtcgacccgg ctctggccgt ggattatgcc aaaatcgcct
      661 gccagcacat ccgcgatgaa gtgggcgtct ccgtcttgga atatacccag gacctgcaga
      721 agctgattac ggtcaacaac tacattgccg gacactgtga tgacgctatt gagcacggct
      781 ggatccaggt ctattatcag cctgtcatcc gcaccctgac cggtgccgtc tgcggcatgg
      841 aagccctggc ccgctggaat gaccccgtcg tcggcttcct gtcgccggcc cgcttcattc
      901 ccatcctgga aaagaaccac gacatctata aactcgactg ctacatcctg cgccagacat
      961 gccaggccgt gagccgccga ctggatcagc agcttcccgt agtcacggga tcattcaatt
     1021 tctcgcgtct ggtctttttc ctctgggaca gcttccaggt cgtcgaagat acggtccgga
     1081 catatggcct gccgcgggac ctcatccatc tggaaataac ggagaccatg ctggtccacg
     1141 ataaagaagt catcaaagag gccctgggac gcttcctcca ggctggctac caaattctca
     1201 tggacgattt tggcagcggc tattcgtcgc tcgacgtcct caaggattat gacttcaata
     1261 tcatcaagct ggatacgcat ttcctctcgt cgtcgacgga caaggcccgt tccatcatcg
     1321 gcaataccgt cgacatgatc aaggaaatcg gcagccagag cctggccgaa ggcgtagaga
     1381 cgcagaaaca cgtcgatttc ctggcctctg taggctgcga taagctccag ggctatttct
     1441 tcagtaagcc catgccctgg aacgatttat tttcctatct ggaaggccag taccggtctt
     1501 tcgaagatgc ggccagccga gcttattacg agaaacccag cgccgccctc cgcaggaccc
     1561 atcagtccct ggccctggtc gaatacg
//
LOCUS       DAWCYS010000402         1577 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3504_length_1577_cov_5.135348, whole genome shotgun sequence.
ACCESSION   DAWCYS010000402 DAWCYS010000000
VERSION     DAWCYS010000402.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_1577_cov_5.135348"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          279 a          424 c          489 g          385 t
ORIGIN      
        1 cgggcgggaa tctataggca atcgcccagc ggggaatctg gacggtattg cccaagcggg
       61 ccggctgggc aaaggaggtg actttgacga ccatgccgtc cgttgcataa ggcaggctgt
      121 cgcggcggtc gtcccaactg tggatgaagg cgatgacgtc ttcgatgttc gtgaacttgc
      181 ggtattccgg attgacctgg aaatggaact gggccaaggc ctggagcagt tcttcctggc
      241 tctcgatggt gatgccgctg tttccgccca aggcgtaggc aaagaaagcc agtttgcgct
      301 gggccgtaat gcgcgggtcg agctggcgca gtgagccggc ggcggcattg cggggattgg
      361 caaaaggttc cagttcgtca tcgcggcgct gctgattcag ggcttcaaac gacgtaatgg
      421 gcatgtagac ttcgccgcgg atctcgatgt gcggcgaatc gctggcgatg tgcaggggaa
      481 tggtgcggat ggtccggacg ttgttggtaa tgtcttcgcc gacgacgccg tcgccgcggg
      541 tgacgccgcg gatgaactgg ccctgttcat aaatgaggtt gactgccagg ccgtcgattt
      601 tcagttcgac gacgtattcc aaatcttcgc cgcccaggtc atcgcggacg cgctggtcga
      661 attcccgcaa ttcatcgtcg ctgaagacgt cgcccaggct gagcatgggc cggtcatggg
      721 tatatttttc aaagccgccg gaaatcttgg agcctacgcg ctgcgtcggc gaatcgggcg
      781 tgacccagtc ggggtgggct ttttcgatgt tttccagttc ccggtacatc atatcgtatt
      841 cataatcagt cactgtcggc ttgtcgaggg tgtaatattg ataactcaat tcatctattt
      901 ttttccgcag ctccaagagc cgcgcttttt cagtatccat gggtttctcc ttcattcgtg
      961 aaaatcattc ctattcgtcc tgtagagcgt ctcatcacaa gttctgtggc gggtcgccca
     1021 cgtggcgacc cctacgaaag cccggccggg gcatttttac gagccgcggg gtgctcgagg
     1081 cgatattgtc ggttttagtc tgtcgcggcg ggtcgccgta tctgattccg aagcgacctg
     1141 cggcctgcaa aagtgcaagc aacctctccg gcccttcggg ccacctccag ctcagctctc
     1201 cgttttggca accgtcctgc gctttcctgt ggtcagcacc tgacgggcca aaaagggcct
     1261 aatatggaag gcttaggctc ccctactgta ttttctctaa gggggcgtat ttcacgacga
     1321 cttggcggac gcctttggtc gggaattgga ttttcatctg catgttcggc ccttcgccga
     1381 tgacgtcgag gacggtgccg atgccccaga ttttatggcg gactttatcg cctttggcaa
     1441 aggtatcggt aacggcatgt ttcttgggca ggctcgacgc gacgggcttg gtcaggatgg
     1501 agacgcgctg tttccccggg accgccgtcg cccggctctg gggcatggcg ctcttgcgat
     1561 gatagtcttc catgaac
//
LOCUS       DAWCYS010000403         1573 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3515_length_1573_cov_4.965744, whole genome shotgun sequence.
ACCESSION   DAWCYS010000403 DAWCYS010000000
VERSION     DAWCYS010000403.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3515_length_1573_cov_4.965744"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          275 a          394 c          474 g          430 t
ORIGIN      
        1 gagaagtata cgattcttcc tgttccagat aggttatccc gtagcgttca cacagctgtt
       61 tcaaggtact gcgcagatag ctcaagctga taagaggaag gagtcttctg gcgaaactgt
      121 gataaaaggc aacctctcag tcagcttcgc tgaccctacg aaccacgaac tacgagccac
      181 aaattatcat ttttttggtt ctaccatata cgtcgtctgg tcggtgaaga tgacgtagcc
      241 cgggacagcg tgggccggtt tcttgacgtg gcgcacgagg gtgcagtcga cttcgacttt
      301 cgagctttcc cgggctttgc tgtagtaggc ggccagctgg gcagcccgtt cgatctgggc
      361 atccgtcggc gtcacattgt ggcaggccag gatgacgtgg gagccgggct ggttcttgac
      421 gtggaaccag aggtcatagg gatgggcttt cttcagggtc aggaagtcat tctgccggtt
      481 gttgcggccg acccagatat cgaggccgtc ggcctggagg gtcaggatat cctgggccga
      541 ttcttttttg agcttgtccc ggttgtggcc gctgatgagg ttcatctgtt tcatttcggc
      601 cttgatatcg gcgatctcat ctttcgtact cgtgttttcc agggcgtatt ccagcgaata
      661 gagatagtcc aggtgtttct ggttctcttc gtggagctgg gccgacatct gcttgcggtt
      721 acgcatcttg gtgtagcgct tgtaatagcg attggcattg tccgtcatgg aataggccgg
      781 attcaaggga atggtcacct tttcctggtc ttccgacagg aggttctgga cagtcacttc
      841 tttttcatgg tcgtgctttt cgtaggcgta gatcatgagc aggtcaccgt agagcttgta
      901 cgtatccatt ttcttcgttt ctttcatttc gctggcaatg cgcttgattt tgcgcttctg
      961 cttttcgatg agcttgccga ccttcttctg cagctgttcc tgttcgccgt tgaggctgcg
     1021 gtgcgtggcc tggtattcgc tgaggtaggt ctcgatgagg ctgaagtggc gccgcggata
     1081 ttgggccagg ctgtgcagtt ccagcgggaa gatgacttcc ttgcccttga ccgtatagac
     1141 gtaagccccg gacatacctt ccaggcgctt gccaaaggct tcgactgtac ggcaccaggc
     1201 gaaacggtct gccggcgaca ggtccccgac gggccgccct gcatcgatgc ccgaatcgtg
     1261 ggacagttcg ttgaggacga tggtgctcat gccgttgaag cgcttgagcc tccacgtctt
     1321 gagcggttcg tccgtccgcg tttccatcat gtccgtcagt tccagggccg aaaaggcaaa
     1381 gggatccatg cgcatgaagt tcggcgggaa ggcatagggt tccttggggg cgatggtccg
     1441 cagggcctgc ttgttcttcc ccgtcttgat gagggcttcg atgatggtgc cgtcttcggt
     1501 aaagatgacg ttgctgtatt tgcccatgag ttcgacatgg attttacgtg tcaccaggcg
     1561 gcccgacatg tcg
//
LOCUS       DAWCYS010000404         1564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3534_length_1564_cov_3.658052, whole genome shotgun sequence.
ACCESSION   DAWCYS010000404 DAWCYS010000000
VERSION     DAWCYS010000404.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3534_length_1564_cov_3.658052"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          431 a          448 c          429 g          256 t
ORIGIN      
        1 cgacggcaag acggccatcg ccggcgacgg tcaggtcacg atgggcaatg ccgtcatcat
       61 gaaagggacg gcccagaaag tccgccgcct ctaccacggc aagatcatca gcggttttgc
      121 cggctccgtc gccgatgcgt tcaccttatt cgaccacttt gaagaaaaat tgaacgaaca
      181 caatggcaac ctcgtccgca gtgccgtcga actggccaag gactggcggt ccgacaaaat
      241 ccttcacaag ctggaagccc tgctcctggt cgctgacagc cagcgcatcc tgctcatttc
      301 cggcaacggc gaagtcatcg aaccggacga cggcgtcctg gccatcggtt ccggcggcaa
      361 ctatgccctg gcttcggcac gggccctcat gcagaacacg gacttgtcgg cccgggaaat
      421 cgctgaaaaa tccctgcata ttgcagccga tatctgcgtt tacaccaacc acaacgtcat
      481 tgtcgaagaa atttaagaaa gaggtatttt catggaaaaa acagaattga cgccaaaaca
      541 gatcgtggcg gaattgaata aatacatcgt cggccagggg gaagccaaga aatccgtctc
      601 catcgccctg cgcaaccgct ggcgccgcaa acgcctgagt gaagacatgc aggatgaaat
      661 catccccaag aatatcctgc tcatcgggcc gactggcgtc ggcaagacgg aaatcgcccg
      721 ccgcctggcc aagctcgtcg gtgcgccgtt catcaaagtc gaagcgacga aatacaccga
      781 agtcggttat gtcggccgcg acgtcgaaca gatgatccgc gacctcgtgg ccaatgccat
      841 ccgcatggtc caggaacagg aaagccagcg ctatgaagaa aaggccgaag aagaagccaa
      901 caagcgcatc ctccaggtct tctggccgaa gaagtccatc aaggaaaaag tcaagaaccc
      961 gatggacgtc ttcttcagca gcgatgatga cgacgatgaa atgccggaag aaccgaagca
     1021 gctggaagag ccgaagtccg accgccggca gaagatgttt gaagacattt gcgcccataa
     1081 gatggacgac cgggaaatcg aagtcgaagt ggctgaacag aaccagggct tccagggcat
     1141 cctgacgggc aattcgacgg aagaactgac caacaatttc caggaaatgc tgggcagcat
     1201 catgccgaaa aagaagaaga aaaagaagat gaccgtcgcc aaggcccggg aaatcttcaa
     1261 ggaagaagaa ttggaaaaat gcctggatat ggacatcatc gtcgacaaag ccatcgaagc
     1321 gacggaagaa tcgggcatcg tcttcatcga cgaattcgat aagatcgccg aaaagggccg
     1381 ttccaacggg cccgacgtct cccgcgaagg cgtacagcgc gacattctgc ccatcgtcga
     1441 aggagctacg gtcaacacca agtacggccc ggtcaagaca gaccccatcc tcgtcgtcgc
     1501 tgccggtgcc ttccatgtat ccgagccgag gggcctcatt ccggacctgc agggccgctt
     1561 ccgg
//
LOCUS       DAWCYS010000405         1558 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3542_length_1558_cov_5.187625, whole genome shotgun sequence.
ACCESSION   DAWCYS010000405 DAWCYS010000000
VERSION     DAWCYS010000405.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_1558_cov_5.187625"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          323 a          470 c          431 g          334 t
ORIGIN      
        1 atcctcatcc tccaaggcac gaaggatgaa atgattccca tcgacctcgt ccgggaattc
       61 gctgaaaaca acgtcatcga attcgttccc gtcgaagggg ccaaccaccc cttccagaac
      121 ccgaaccaca tggccctggc catccacaag atcatcgaat tcttccatga cgacaaggcc
      181 tgatggtgcc accgccgttc ctagaacgag ccgccaccgc cgccgtggtc gtcgtcgctg
      241 cagtcagcgt cactgtcgaa atcaggcagg tcatcatcgg ccggacgggc cttggcaatg
      301 tgctggacgg tcatgaagac gaacgtatcg ctgctgtcgg tcagatggaa actgccggcg
      361 tcgacatagg catcagccgc cgcggcttgg cggacattgc tcatccggct gatgagatag
      421 gcccggaacg ccacgccgcc acccacagcc aggacgaggg ccacgaccag ggccaggggg
      481 ctgaaggcat cggccgggtc ataggccttg cccttttcct ggtaataagc caatagtttt
      541 gccgacgact ggccataggc ctcgaaggct ttcgcataat gaccgtcctt caaggccggc
      601 aggaaggcat ccttgagata agggatgcct tttttattgc tgattttctc cttcatggcc
      661 ttgtctgtcg caacatacca gtcatggcgg tccatggcca ggagcaggac catattgccg
      721 ttctggccgt ccttatagtc ccggtccagg agctgattgg cataggttcc ggccttgacg
      781 cccttcgtcg aggcgacggt gactacagcg acgcgtacac catatcttgc ttctgtatac
      841 tgcaagacgg ccgtcacttc agcctgttct gccggcgtga gtagatgggc gccgtcggac
      901 aggctcggcg ctgccgctgc ggcaccagcc gtcgtcagga ccacgacaac tatggcaagg
      961 attgtttctt tcaaccattt ccacatcgta ctcactccct tcataacagg cccgaccaga
     1021 ccatgacgaa tttcaccagg aaatacagga ttggtaccag gacggcggcc agggccgcgc
     1081 cgtattcata ggacttggcc ttgtcatacc gcagggaacc gaccatcctg ccagtctggc
     1141 cattcatcat gaaggtgtag gctttgccgt catagcgcgt cgtcaagatc cagaccggcg
     1201 ccatggcgta ttccacggtc ccttcgtctt tatggacgac gccgtcttca aatgtccggc
     1261 gctgatgatt gtcgactgtc tcttccaaca tggcgaaggc actctgcgac agccgttcat
     1321 cggccctggg gacggcagcg tcggcgtcga cgtcgtattt atcggccaag taaccggcga
     1381 aataggccgg actgaacggg accatttcgc cgtagtcaaa gggctcgatg ctttccatat
     1441 atgtatcgtc catcttctgg ctgccgtcga cagggatgcg atggaaggcc agagagcccg
     1501 tccggtcaca gtggtagatg ctggtttcga taatcgtttc gtccggcgtc tcaaagac
//
LOCUS       DAWCYS010000406         1555 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_6123_length_1555_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000406 DAWCYS010000000
VERSION     DAWCYS010000406.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6123_length_1555_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          304 a          506 c          452 g          293 t
ORIGIN      
        1 aacttgtcct tccgcgaggg cgagcgcctg gccctgaaga ccttcaacac gcgcctgggc
       61 atcgtcggcg gcctggccgt cctggggacg tcgggcatcg tcgaccctat gagtgaacag
      121 gccctggtcg atacgatcca tacggaaatg gattcccgcc gcgctgccgg ccagacccat
      181 ttattggcct ttttcggcaa ttacggcgtc gatttcagcc gcgaccatct gggtgtcgac
      241 gtcagccagc gcgtgaccat cagcaactat gtcggcgaaa cccttgatta tgccgcctac
      301 aaaggctttt ccgacgtcct ggtcatcggc catatcggca aattggtcaa agtcgcccaa
      361 ggcatcatga atacccatta ccattatgcc gacggccgca cgaccatcct ggccctggaa
      421 gccgtctttg ccggtgcctc acggcagctg gcccgtcaga tttacgacag cctgacgacg
      481 gatgaagccg tgcgcctgct gaaggaacaa aacctgctgg aaccggtcat ggccgcagtc
      541 tgtgagaaaa tcgaggccta tatggaccag cggacccatg gtgaaatccc gacggcggcc
      601 gtcgtctttt ccaatgccta cggcgtcctg ggcctgacgt cgcgggccaa agaatttctt
      661 gcattgcata cgaaggggga actacgatga tacactttgt cggcgccggt cccggtgctg
      721 tcgacctcat taccgtccgc ggccagaaat tactggaaac agcggaccag attatttacg
      781 ccggctccct ggtcaaccca cagctgctgg cctgtgccaa agaaggctgc cgcatcctcg
      841 acagcgccgg catgacgttg gaagccgtcg tggcggccat ggaagaaggg gagggccagg
      901 gctggatgac cgtccgcctg catacgggcg atccatcagt ctatggcgcc atccgcgagc
      961 agatggacat cctcgcccag aagggcatcg cctatgacgt cgtacccggt gtcagctcct
     1021 tctgtgccgc tgccgcgtcc ctccaggccg aatataccct gccgggcatc agccagtcgg
     1081 tcatcatcac ccgcatggaa gggcggacgc ccgtgccgga taagcagaaa atcgccgatt
     1141 acgccgctca tcaggcgacg atggtcattt tcctcagcgc atccctcttg gacggcctcc
     1201 aggccgaact cctgcgcggc ggctatgcgg cagatacgcc ggcagccatc gtctacaaag
     1261 catcatggcc agaagaacag acttatacct gcacggtcgg ccccctggct gaaacggccc
     1321 ggcgccatgc catttccaag acggctctca tcgtcgtcgg caaggtgctg gggccggctt
     1381 atgaccgcag ccttttgtat catccggcct ttacccacgg ctgccgccag gcggcgccgg
     1441 aggaccagca gtacggcgac cacatcggtc aggagacgga accatgaaag ccgtcgtcct
     1501 gtcctttacc caggccggga cggccctggg ctgccgcgtc gctgcctgct tgcgg
//
LOCUS       DAWCYS010000407         1547 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3570_length_1547_cov_4.505362, whole genome shotgun sequence.
ACCESSION   DAWCYS010000407 DAWCYS010000000
VERSION     DAWCYS010000407.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_1547_cov_4.505362"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          488 c          411 g          301 t
ORIGIN      
        1 ggcatgtgct gccgccgcct gggactgcag ggtatcggcc acatcgcggc taaccattcc
       61 gtctgggacc tggaactgga aaacctgtcc gtcgaatcgc tgttgctcat ttatgccgat
      121 ttccgcgtca agagcagccg cgatgcccag ggaagggaaa tcgtccattt ctattccctg
      181 gcccaggcct tcgacgtcat cctggggaag ctggataatg tcgacgacgc caagcgccag
      241 cgctatcaga aagtctatgc taaattggcg gactttgaag atttcatgaa agaacacggc
      301 gccgtcaccg aactgcctgc cgacttcgcc tgggaaccgg ccgacccgcc gcagcccatc
      361 catcgggaaa aagtcctcct cgaaggcaat gacgtcattg cccagctcaa atatgccgcc
      421 atcgaccaca acatccgcgt cacgagcatc ttccgtgagg aaagcgattt tggcaacctc
      481 atcgaagcgg cccgcagcga gcggcagtgg aagaatgtcc gcacgtatat cggcatcttc
      541 gaggaatatt cgacgtacat gacggagcat cagaagctca tgaccctgaa gttcctctac
      601 gagctcttgt cccaccgcga aggcgacatc cgcctgcagg cggcagccct catgggacgc
      661 atcatcgcca atttcaacga tacgtatacc aaggaactgc ccgaaggcgt ctccctgccg
      721 gccaaggccg tgaccaacct gtccctgttc acgacctacc tggaccgcat catctgcccg
      781 gatatccgct tcaccgagca gcacaagctg tggatcggct actgcctgag cacctttgta
      841 tcggccgtcc tgcaccgctg tgcccggtcc cagcgtcccc gctatacggc cgtcatgggg
      901 ccgtattatg aacggcgcaa ttacagtgaa gaagagtata tcatcctctt gaaggtcctg
      961 gccgacatgg accgggacta tctggacgaa gcctttatgg ccgccgccgg ccccttcata
     1021 gaaagggcca agcagagtga atccttcaac ctgcgcgtgg ccgccctgaa ctgcgcccgc
     1081 cacgtctttg ccgactatac ggatgacttg tactataagg acctgctcaa ggtcatgaac
     1141 ctgcctgaag ggcgggacgc cttctttgaa cgggaaggca acctcttcct ggccgacctc
     1201 aagatgggga cccattgggt cgtcaaagtg gccaacctgg aactcatgct gcgctacatc
     1261 gacggccaga gcgatgccgg cagcgtcatg catctcggga cccatctgac caatctgctc
     1321 aaggtcagcg aatccatctt cgtccgccgc gccgccggca acagcctgct caagattgcc
     1381 tcgaacatga cttatgccca gcgcaacgaa ctgacggtcg aactgttcaa cggcctggaa
     1441 atcggcgacc cgcagatttc taaatacgtg ccggaatttt tgggcaagat gatcctcaag
     1501 ctgccgccgc gggagtgcga cgaattcgtc aagaccatcg aaggcca
//
LOCUS       DAWCYS010000408         1543 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3573_length_1543_cov_7.228495, whole genome shotgun sequence.
ACCESSION   DAWCYS010000408 DAWCYS010000000
VERSION     DAWCYS010000408.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_1543_cov_7.228495"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          321 a          421 c          486 g          315 t
ORIGIN      
        1 atgaggcgcc gcggctcttc gatgacttcc atggcaaagg ccacgttttc ctgagccgtc
       61 ttattgggca ggaggcggaa atcctggaag acgatgccca ttttacggcg cagatagggc
      121 acgcggttct tggccagctt attgacttca atgccgttga cgaagaccga accccgcgat
      181 ggcagttctt cataggacaa gagcttgatg agcgtcgact tgccggcacc gctggcgccg
      241 acgacgaaga cgaactcgcc cttgccgata tggagcgata cgtggtccag ggcaatggag
      301 ccattgtcat agaccttgga tacgttcttt aattcaatca taaggacctc cgatgctgtt
      361 gcaggcgctt gcggacgccc ttgcggcctt ccaggatgcg cagggccaga taggcattgc
      421 gcagggactg gcgccgcagg tgattgaggc gggccttgat ttccggcgcc aaatggccga
      481 gagccaattt ctggctcacg tcggcgacga tatccgcagc ctggacgtct tctgtcgtgc
      541 cgcagaccct atcgccgatg aggtcgatga aactgtcgat tttcgggtca tacgacacgg
      601 ccgtcgccgg cacctgcatg atagccgaaa agatgagggc atgcaggcgg ttggcaatga
      661 ccgcatccat acagcccatg agggacatga attccaccgt cgtatagtgg ccgtcgagga
      721 cgacggcttt cgtcttcatg cggctggcaa tgtcgcggcc ggcttcgacg tcggccgggt
      781 actgcatggg gatgaagatg acgtggacgt cctgtgtttc ctgcagctcg tcaacagcag
      841 cggcaatggc gtccttatag gcttcatggt cctgccagtt gcggacggca atgcccaggc
      901 gccggcggat gccggtgatg ccgtgtttct tgagaatata gaggccgatg ttcttatcga
      961 cgggatgcat ggacaagacg gcgtcagccg tgacatggat aggcggccgg gtcacgccca
     1021 tggcggccag ttcggctttg gaatcggcat cgcggacgcc gatgacggcg acgcgctgca
     1081 agacatcgcg cacagcccgg cgggccttgg caccgcggac ggggccgatg ccctgagcgt
     1141 agagcatgac gggcttcttg aagaacaggg cgattttcat gatccacaag taataataga
     1201 ggctgcgggc gctggtcacg tcctgcaaaa ggctgccacc gccgcttata agaatatcac
     1261 agcggcgaat ggccgctgcg atagcaaaga agttcaggcg gtgtacagcc tgtacgttat
     1321 ggttttcccg ggtcatgcgg cagttgccgg tgatgaccgt aatcgatacg tcgggaatga
     1381 tgtctttcag ggagccgaca atggccgcca gcatggcttc gtcgccggca ttggcaaaac
     1441 cgtaatagcc ggaaataacg actctagtca cgatgggcca cctgctttcc cagccacgtc
     1501 gtcacatagc tgacgatggc aatggcgatg atcaggccga tac
//
LOCUS       DAWCYS010000409         1536 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3588_length_1536_cov_7.027009, whole genome shotgun sequence.
ACCESSION   DAWCYS010000409 DAWCYS010000000
VERSION     DAWCYS010000409.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_1536_cov_7.027009"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          454 c          413 g          298 t
ORIGIN      
        1 taggaggaat tcgttttggc aacgtcaccg acaaagggtg tgaaatatat ttcggacaac
       61 cgcaaggccc gtcacgatta ttttatccat gaaacgtatg aagccggcat tgccctgacg
      121 gggacggaag tcaagtccct gcgccagggc aaggtcaatc tgaaggacag cttctgccgc
      181 atcgagaacg gcgaagtctt cctcctgggc atgcacatca gtccctatga tcagggcaac
      241 cgcttcaacc acgatcccct gcggacgcgc aagctcttgc tccataaata tgaaatcatc
      301 aagctcctgg gcaagatcca ggaaaaaggc tataccctca tcccgctcaa cctctatttc
      361 aagaagggga aggtcaaagt gaccgtcgcc ctggtaacag ggaagaagct ctacgacaag
      421 cggcaggccc tggccgaaaa agaagccaag cgcgacatcg aacgccgcat gaagagccgt
      481 aactactaat caggaggaga agtatctatg aattcagaag acccaaaaat cctttcggat
      541 cacgaacggg atagtttccg cggccagacc atcgatgaag atgggacggt ccaagaccag
      601 aagtccctct atgaagaacg gctgcgtcag caacaggaag cgcatcatgg catccacatc
      661 gtcacgtccg gcctttcgtg gcgtgtcaaa ctggccctgg gcttcatcgt cttctgcatc
      721 gtcgccgtca tcatcggcgc cctgggcttt atcatcatgg ccatgcccta tctcttgggc
      781 atcttcgtca tctactgtat ctacagcatc gtcagctccc tgttgcatcg gtgagtgggc
      841 gtagggaggt tctatgaaag aaaatatcgt catcatcgac ggcagcagtc tgctctatcg
      901 ggcattttat gccatcccgc atctcaccga tgcccagggg cacccgacga atgccgtcta
      961 tggttttttg aatatgttat tgaaattgta cggagaactg gacccgcagt acgttgccgt
     1021 cgccttcgac aagagcaagc acaccttccg caacgacttg ttcgacggct acaaggctac
     1081 gcgcaagccc acaccggatg acctgcggcc ccagttcgcc ctgatccgcg aagtcctggc
     1141 ctgcctgggc atctgcgtcc tcgaacagga aggctacgaa ggggacgaca tcatcggcac
     1201 cctggcccgc cgcacggcag ccgatggcta tgccgtcgct gtcgtcaccg gtgaccgcga
     1261 cgccctgcag ctggtcacgg acgacgtgac ggtctatctg accaagaagg gcatcaccaa
     1321 catgctggcc gttacgccgg ccgtcatgga agccgaatac ggttatacgc cgggccaggt
     1381 catcgacatg aaggccctca tgggcgatac ggccgataat atccccggcg tccccggcgt
     1441 cggcgaaaag acggcgctca agctcatcag ccagttcggc agcgtccacg gcgtctacgc
     1501 acacctggac gaagtgaagg gcaagaagct gcagga
//
LOCUS       DAWCYS010000410         1530 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3611_length_1530_cov_4.496271, whole genome shotgun sequence.
ACCESSION   DAWCYS010000410 DAWCYS010000000
VERSION     DAWCYS010000410.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3611_length_1530_cov_4.496271"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          300 a          405 c          468 g          357 t
ORIGIN      
        1 tcattagacg ggctgattct ctgtgtagcc aggtcgatct tggcggctga gacgagagac
       61 gtgccgagca cagatccctg ggcagccagg aaggtgttgg ccatgccgcc gccgataatc
      121 atgaggtcga ctttcggcag aaggttggaa atgacttcga tcttgtcgga aactttagcg
      181 ccgccgatga tagctgcgaa ggggtgagcc ggattatcga cagcaccgcc gatgaagcgg
      241 atttcttttt ccaggaggaa gccggcagcc atgggcagga acttcgtaat gccagcgacg
      301 gaagcatgag cgcggtggca gcagccgaaa gcgtcgttga tgccgacttc agccagggaa
      361 gcgagctggc gggcgaaatc ttcgccgttc ttttcttctt ctttatggaa acggaggttt
      421 tccagaagca ggacttcacc cggtttgagg gcatcagcca tggctttcgt ttctgcgcct
      481 acgcagtccg gagcgaattt gacgtcgatg ccgaggagtt ccgacaggcg tttagcgacc
      541 ggtttcaggg aatacttttc gacgacctgt cccttcgggc ggccgagatg gctggccagg
      601 atgacagcag ccttgtggtc gagcaagtat ttgatggtcg gcagcgtctt ctggatacgc
      661 gtgtcatctg tgatgtggca gttttcatca agggggacgt tgaagtcagc gcggacaaag
      721 acgcgtttgt tttcgacatt tacatcggtg atcgtttttt tcatgaaagg ttcctcctcc
      781 aattaaagca gttgtatgat tttttcagcc gcaccttcat cagtgaccag cgtgcccgta
      841 tggcaggccc gcatgacgcc gacgatggct tctgccttgc tggcgccacc ggctacggcg
      901 atgatgtgcg gatgatattt gatttcccgt aatgaaatcc ctacattatt cgtaccatat
      961 aagattttgc cgtccaggcc gcaatagcag cccagcgatt cgccacaggc gccgaggctg
     1021 tgcagggcat cgcgctggag cgccgtcatg ccccgcttgc gggccatgcg gtcggcccgg
     1081 ccgatgccga agatgacgac ctgggcccgg gcggtcatgg cgacattttc ctgggtctga
     1141 cggtcagctt taagcatcat ctgcagcgcc gagtcgctca ggccttcggg gatgtggatc
     1201 atgcggtacg agctgtgagt cttggacgca atgttggcgg caatatagtt ggcctggact
     1261 tcgacgtggt tccccatgct gccgcgggcc ggcaggatgg tcacattggc cagacattcc
     1321 ggcagcatac gggccacttc ggccatggtc gaaccgccgc tgatggcgac agtatcgacg
     1381 ctgccaaaga ggtcgttgag caagagggcg ccacggcggc ccagttcttc cttcgctgtc
     1441 ttatcgctgt cactgtcacc gggaacgatc tagacattct tcaggtccag cttctggcga
     1501 atctggcgct acatgtcatc aaaatagttc
//
LOCUS       DAWCYS010000411         1527 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3621_length_1527_cov_4.283288, whole genome shotgun sequence.
ACCESSION   DAWCYS010000411 DAWCYS010000000
VERSION     DAWCYS010000411.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_1527_cov_4.283288"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          274 a          422 c          509 g          322 t
ORIGIN      
        1 ggttaggggt taggggctca caggtcgaaa ccacgagcca ctaggcctca ggccgaaacc
       61 acgagccact aacaacgcgc ttccagcttc tgcccgtcag tgccgatgag ggtgacttcg
      121 acgacggtgc cttcggcatg aggcggaaga ttttcgacga cggcccgttc gtagttctcc
      181 gtaaagcctt cgcctgtcgt gccgtcttgt ttttcgatga ggaccgaggc cttcttgccg
      241 accagggccg tcaggagcgc cgctttctgg cgggccgaca gttcattgac gagttcgacg
      301 cgccgtttct tttccgccgt ttcgacttgg tccttcatat ccgctgccgg cgtccccttg
      361 cgctgggaat acgggaaggc gtggatatgc gagaaagaga ggcgttccag cgtcgccatg
      421 gtgtcctcga agagggcttc cgtttcgccg ggaaagccca ggatgaggtc cgtcgagaca
      481 gtcaggccgt cgatgcggct gcgcaggcgg gcgatgaggt cctcatattc agccaccgtg
      541 taatggcggt tcatggcctt caggatcggg tctgaaccgg actggagtgg ccggggtaga
      601 ggcgggcaga gccggtccga gcggttcatc acgtcgagca tggcgtcgcc gatttccagg
      661 gactcgatgg aacccaggcg gatgcgggcc acgtcggttt ccttgaccag gcggtccagg
      721 atgtgggcca gcgacggatg gtcgttgagg tcgcggccat aggcgccgag gtggatgccg
      781 gtcaggacga tttccttgaa gcccgctgcg accaggcggt tggcttcggc gacgatggcg
      841 tcgggctggc gcgatttcaa agcgccccgc gtatagggaa tgatgcagta ggaacagaaa
      901 ttgttgcatc cttcctggat cttcaggtcg gcccgtgtgt gttccactgc cgacggatag
      961 agggggattt cctcgaattc atcgtggcgg cgcacgtcgt ggacggccgt gatggcttcg
     1021 tcgtcttcgt cgtgcaggag ttcgcggaca atggatacga tgcgcttgcg ttcattggtc
     1081 ccgacgacgg cgtcgacttc gggcatggac gtcaaaagct ccgggtcgag ctgggcatag
     1141 cagccggtga cgatgacctt ggccgcatcg ttgcgcttct tcgtccgccg gatgagctgg
     1201 cgcgatttct tttcgccgac ctgagtgacc gaacaggtat tgatgacata gacgtcggcc
     1261 gggccattga aatcggcttc cgtgaagcct gcagccagga agaggccgcg catactgtcc
     1321 gtatcgtact ggttgacacg gcagccaaga gtggcaaaag cgatagttgg cattacttga
     1381 atcctctttc gtacataatg atagacaggg ccgccagcga cgccgtttca gcgcgcagga
     1441 tgcggggccc gagagagacc gtgcggcacc acgacagacg ctgcatggca taggcgattt
     1501 ctttttcggc atagccccct tcgggac
//
LOCUS       DAWCYS010000412         1522 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3634_length_1522_cov_5.769598, whole genome shotgun sequence.
ACCESSION   DAWCYS010000412 DAWCYS010000000
VERSION     DAWCYS010000412.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_1522_cov_5.769598"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          356 a          411 c          395 g          360 t
ORIGIN      
        1 gcctccagcc ctggaataag cagagcgctg actgactggc aagccgcgtc ccgtatggcg
       61 tggaactgac gccgcaccgc ctggctcaga tcgatgcggc cgaagccgcc gtagcgcctt
      121 acgtccaggg gtccctgcgc gtccgctatc acggcgatgt agcccgcatc gaagtcgccg
      181 tcgatgaaat cccgtcggtc ctggctcatc gggaggaaat cgtccgggcc atcaaggata
      241 cgggctttac gtacgtcacc ctcgatttgg gcggctatga aatgggcagc ctgaacgacg
      301 taatccatac tgatgaggaa gtaaagtgat atggctattt ccaagaaagt aaaagatgaa
      361 atgctgcagc ggttccagga ccagtacggt atcatgaaac cggctctgca ctataattct
      421 ccttttgaat tgatgattgc cgtcatcttg tcggcccaat gtacggatga acgggtcaat
      481 atcgtgacgg ccggcatgtt cccggaatat aacacgccgg aaaagatgct gaccctgggg
      541 acagcgggac tggaagaaaa gatacggacc tgcggcctct atcgcagcaa ggccaagaat
      601 atcctggcca actgtgccat cctttgtgaa aaatatcatg gccaagtgcc gcagactttc
      661 gatgagctgg tgaaactgcc cggcgtcggc cgcaagacgg ccaatgtcct ggtcagtatt
      721 ttgttcaata cgccggccat tgctgtcgat acccacgtct tccgtgtgtc caaccgtctg
      781 cagctggccc aagggacgac gccgctggaa gtcgaaaagg gcctgcagaa ggccattccc
      841 aaggaatggt ggagccgggc ccatcattgg ctcatctggc atggccgccg ggtctgcaaa
      901 gcccgcaagc ctttatgcga caactgcttt ttggctgacc tttgtcccag ttgtcagaag
      961 taaaacgtta aatcaaaata aatacattga caagtcgtta aaaattttat aaaataacac
     1021 caatacagac aaatttcata agttatatcc agagcagctg agggactggc ccgatgacgc
     1081 tgcggcaacc tgcctaaggt aaggtgccaa ttcctacagg ctattcctga aagatatcat
     1141 tatgactgag gcgcagcttg gaggcagcgc ctcttttctt ttggcgtatt atgtacttat
     1201 tttcacatca ttcaatcatt cttagggggt attttcatga acaacaactc ttctggcggc
     1261 aaggctatcc gggggctgga tgtgctcatc ctgggattcg ccttctttgc cacctatttt
     1321 ggcgccggca atttgatttt cccgccccag ctcggtttga acagcggcag cgatttcctg
     1381 tccggcctgg ctggcctgac cctttcgggt atttttcttc ctatcttcac gctcatcgtc
     1441 atcggcctca acggcgacgt ccactccatt actgaacggg tagggaagaa tacgtataac
     1501 gtcctcctgg cagccctcat gc
//
LOCUS       DAWCYS010000413         1521 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3637_length_1521_cov_4.607776, whole genome shotgun sequence.
ACCESSION   DAWCYS010000413 DAWCYS010000000
VERSION     DAWCYS010000413.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3637_length_1521_cov_4.607776"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          384 a          418 c          352 g          367 t
ORIGIN      
        1 ccctatcagg tattcatgaa ccggtcggac atccccggcg gctcgaccct gggctccctg
       61 ctctcagcca atctccccct gcgggccatg gatatcggca tccccattac ggccatgcac
      121 tcgtgccggg aactgatggg cgctgccgac caggaaagcc tggtccgcct gattcgtgcc
      181 tttttcagcc ataaatagct ttttattcta tactccgtat acatctctat gtttttatca
      241 atatttttct taatttaagt atcatttttc gtcccaatat tatatttaag cataatactt
      301 gcaagttctt agaatcatac ggttgacgct ttttatcatc catattataa tgatttccat
      361 attataatga tcagtgtata ttacattgtg ttgtatagta tacgaaattg aatgtgtaca
      421 acagagacta ttgtgtacag aaagagtgaa cgcgcatgac tttaggacaa gaaattagat
      481 attatcgcaa aaaaaagggc ttaacccaga cggctttcgg tgccctcttt ggcatgtcca
      541 aacaagccgt ctattcttgg gaaacgggcc tctattcacc ggatatctct gtattattga
      601 agatggctga tttcttccag attcccatct gtatcctcgt cggccggccg ggcatgtact
      661 gcaaaggcag cgaagagcac ggcaacagct gcgacttctg tgcttccatc accgaagacg
      721 acagccacat catccgggcc atccacgcct tgccgccgga aaaacaggag gccattaaaa
      781 cattattgaa tatcaatggc aaaaaataaa gtgttacaaa aagaggctgt cgcattaaga
      841 cagcctcttt ttgagtggtt agcagctagc ggatggattt aaatttaaag ttcatgcaaa
      901 tccgattcac aagtatctca catatcccct ggcgggctcg ccacgtgcga gcccctacga
      961 gccacgaacc actagccacg agccactaaa ccatggtatg atggaagaaa aatgtaaagg
     1021 acgatgtgtg ttatggaatc tctcatccgc tcggtctacg gccgggccga cgctgaatat
     1081 gtcatcaaga agtcccgctt catcgcgacg gtctgcgaag tgaaatccga agacgaggca
     1141 gcagccttca tcgaggaaat gaaaaagaaa tactgggatg cccgccacaa ctgctcggcc
     1201 taccaggtcg ggccggacgg ccgtttccag cgttccagcg acgatggcga accagccggt
     1261 acggcaggcc gtcctatcct ggaagtcctc aagaagcagg gcctgaccaa tacggccatc
     1321 gtcgtcaccc gttatttcgg cggcatcaag ctcggcgcca gcggcctcat ccgggcttac
     1381 agccacgctg ccgccctggc cctggaagcc gcggatatcg ccgtctatac gcccttcacg
     1441 atcctgacgg cgactgtcgc ctatcccctg gtcagcacga tggaacgctt cgtccccgac
     1501 cacggcggca ccattaccgg c
//
LOCUS       DAWCYS010000414         1519 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3640_length_1519_cov_3.643443, whole genome shotgun sequence.
ACCESSION   DAWCYS010000414 DAWCYS010000000
VERSION     DAWCYS010000414.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3640_length_1519_cov_3.643443"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          339 a          473 c          393 g          314 t
ORIGIN      
        1 cgcgccaccg catcctctcc agccatacgg acgacgaatg tcagatcttc ttcctcgaca
       61 cgccgggcat ccacaagccg aaacataagc tggtggaatt catggatgaa gcggcctatc
      121 agtccctgcg cgacatcgac gccgtcctgt tcctcatctc gggcaatgaa aagaagggcc
      181 ctggcgacct cttcgtcctg gataagctga aagacgtcgg cgttccggtc ttcttgatca
      241 tcaacaaagt cgacctcatg acgaaagaag agatactgca caagattacg gaatatgccg
      301 aactctatcc tttcgcccag gtcatcacca tttccgccct gaaaggggac aacgtcgatg
      361 ccgtcgtcga cgaactgcgc aagatcctgc cggaagggcc caagtatttc cctgacgaca
      421 tgatcacgga ccagccggaa cgccttttgg tcgctgaaat cgtcagggaa aagctcttcc
      481 gctgtacccg cgatgaagtg ccccatgcca tcgccgtcta tgtcgaagaa atgaaggacc
      541 gcggtcacaa taaggtctat atccgggtca cggtctatgt cgaacgggat tcgcagaagc
      601 gcatcgtcat cggcaagaac ggcacggtcc tcaaagaagt aggcaacctg gcccgccagg
      661 aaatcgaaaa cctcctcggt tcttcggttt acctcgacat ctgggtcaaa gtcaagcggg
      721 actggcgcaa taagtcgggc gccctcagcg aattgggcta taagaacgag tagggggctg
      781 catgatggaa gtacaatcaa tcgtccaatg tctgctcgtc tttctcatct gtttggccgt
      841 cagcgccatc tgcgtcaccg gtaaattcgc ctttgcccag ctgcgccggg aatacctgga
      901 agaactcgtc cgcgacggcg atacgtcgct gaaaccgctc ttgtccctct acgacaagcc
      961 catcgtcttt ctggggacgg cccagctcgg catggccttg tccggcctca tcgccggcgc
     1021 tgccggattc tgcggcctct atggcctcta cggcctgctg gctccgtata tcggccatac
     1081 gtggctcatc tggctcatcg aaatctgcgc cggtatcgtc ctctgcttca tcctctgggt
     1141 ctttggcgag ctcatcccca agtccatcgg cctccagcgc ccggaaaaga gcctcaaggc
     1201 cctgcgccgc ttcatccagc cctggagccg gcccttcatg cccttcatcc tcttcgggaa
     1261 ctggctgggc cgggccatcc tggcagaccg caacctcgat gtgaccaatg aaatcgatat
     1321 ggaccattcg gaagatgaaa tccgcatgct ggttacggac atccacaagg aagggaaaat
     1381 cgaccaggtc gaaagcgaac tcatcggcaa cgtcttcgat ttcgccgacc gcctggccaa
     1441 ggaaatcatg gtgccccgcc aggacatcgt ctgcctctat acggaagatc cgatgaacca
     1501 gcatttgcag accatccgc
//
LOCUS       DAWCYS010000415         1519 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_3806_length_1519_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000415 DAWCYS010000000
VERSION     DAWCYS010000415.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3806_length_1519_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          355 a          493 c          407 g          264 t
ORIGIN      
        1 acggcgccgg caagacgaca ctcctgaaaa tcattgccgg cctgaccgac accgacagcg
       61 gcacgatctg gcgcaatccc aaggcgcgca tccattacct gccgcaggaa ccgcagctcg
      121 tgtcgggcca tacggtcctg gaacgcgtcc tcgacggcga cctgccggtc atgaagctcc
      181 tccagtgcta cgaagaagcg gtccagcgcg atgacgatgc agccgtcgtc gccctcagtg
      241 ccgaaatgga cgaacagcag gcgtgggaac tggaagccca tgccaagatc attctcagta
      301 agttgggcag ttccaatatt tccccgtccg tcgcctaatt gtccctcggc ccgcgcaagc
      361 gcctgggact ggcccgggcc ctgatcatgc cctgcgacct gctcatcatg gacgaaccga
      421 cgaaccacct cgacgacaag accatcatgt ggctcgaaga atacctgcgc gacagcaagg
      481 ccgctatcct gctcagcacg catgaccgct atttcctgga ctgcgttgcc gattccatga
      541 tcgaactcga ccgggggcac gtctatacct ataccggcaa ttacggccag tacctggaac
      601 agcgccaggc ccggctcgaa cgggaagaag cgagcgaagc caagcggcgc aacctcatcc
      661 gccgtgaaaa ggcctggatc cagcgcggcg cccaggcccg gtcgacgaag cagaaagccc
      721 gtaaggaacg gtatgaacag ctctgcgcca tcgaagcggg caaacccatc ggcgaagtgg
      781 aaatgttcga cacgtccgtc cgcctgggca agaccatcat cgacatcgac gacatggcct
      841 tcggctacga cccggcccat cctttgttcc agcacgtgac ctatcacgtc gtcaagcacg
      901 accgcatcgg catcatcggc gagaacggcg tcggcaagtc gacactgctc aaagtcctgg
      961 ccggccagct ggcgccgacg gaagggacag tcactatcgg ccagaccgtg cgcttcggat
     1021 tctttaccca gcagctgccc cagttcgacg aaggcatgcg ggtcatcgac tacgtccagg
     1081 aacacggcca tttcgtgacc aaccagttcg gccagcgcat ttcggcgacg cgcctgctgg
     1141 accagttcct gttcactgaa gaaatgcagt atacctacat tgccaagctg agcggcggcg
     1201 aacggcggcg cctgtacctg ctgcgcctgc tcatggacca gcccaacgtc ctcctcctgg
     1261 atgaaccgac gaacgacctg gacatcccga ccctgaccgt cctcgaacgc tacctcgata
     1321 ccttccaggg cgtcgtcctc gtcgtatccc atgaccgcta cttcctggac cgggtggttg
     1381 ataaactttt cacattagaa aaggggacat gggaccgctt ttacggtgac tatagtgaat
     1441 acttggaaga aaaatacagt catcaccaag aaaagaaaag cgaaaagccc gtttcacctt
     1501 ctattactaa aacgaatat
//
LOCUS       DAWCYS010000416         1517 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_9386_length_1517_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000416 DAWCYS010000000
VERSION     DAWCYS010000416.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9386_length_1517_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          397 a          413 c          391 g          316 t
ORIGIN      
        1 aacgaggacg ccgcaagtgt cgcagtcaca accatattta gcgcgggtat agccgatgtc
       61 acgaatcgtt tcgcggacga tgtgcgggat atcgacgtag cacgtcgtag aaatttcacc
      121 gacgacatgt accataccag tcgttaccag ggtttcgcaa gcaacgcgag catttttatc
      181 ttttgcgaga atggcatcca aaatgctgtc cgaaatctgg tcagccattt tatcgggatg
      241 gccttcggtg acggattcgg atgtaaagaa ttcacgattt tttgccatga gtgatcctcc
      301 ttaaaaaaaa ttcccctcat ccatgacttc atgagagggg cttctaatcc ccatctgcca
      361 gtatatcact gctggaatta gcacctttca tagctgaagg ttgctgcgac atcacagggc
      421 cagtccctcc gtcgtctctt gatggttctt tatttggttt gctcatccat tataattcaa
      481 gtccgcctat tttgcaagtg ttttttatgc ctaaaaaaca ttgtctacta gacgttgccc
      541 tataggcagg gttataatgg agaagataga aaggatgatt agatatgacc aaattgcggc
      601 ctagagatat attcttcaac gacccttatt ttgattatgc aacctgcagc aagcccggcg
      661 acctgcggct ggccaagcgg gtagaagtat tcggcaaatg gtacaagacc ccgagcctgg
      721 tgacgaccct tctttcccag gaacggctga ccttcagccg ccacaccggc acagaccagg
      781 aagacgcctt ggtactgcgc atctacgaac ggacggccga ccggctgctc atcaatacgt
      841 attatacgtc caaacagaac ccggaagaaa tctacgccgg cacgatccac ctcgaacgct
      901 acgatatggc gcagactatg gaatcttttt cctatatggg catcatcgac ctgctcatgc
      961 tccagacgga cagtcagatt ctcggcttga ccgacgtctt tgaagacgtg cagatggcca
     1021 agcacgcggc agcccatgcc ctgggcatct atctcatggt catctgcgac cagccctggt
     1081 ccgacgaagc cgtcactggc ggccaggaaa cgccggccat gatgatgggc aaagaaaaag
     1141 aagaacttca gcagctgcgg gaagaaaaag cccagatgga cgccgaccgg gaagaactcc
     1201 tgcgcctgcg cctggaagcc aagaaccgca cggccgtcct ttctgaactg gaacggctgc
     1261 gccaggaact cgatgcggaa aggacgggcc gcgaacagga ccgcaaacag tatgaagacc
     1321 gtatcatcaa gcaggaacag tatatcgaaa agataaagga aacggcatcc agccagattg
     1381 ccgaactcct ggccctgcgc aaggaactca acaagcgcca ggtgcggccg gatatgacca
     1441 aaggccaggc gccgcggaaa gtgacgcccc aggaagcgtc gcgttccgtc cgggacccgg
     1501 aagaccgcgg cctggac
//
LOCUS       DAWCYS010000417         1515 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3651_length_1515_cov_6.302055, whole genome shotgun sequence.
ACCESSION   DAWCYS010000417 DAWCYS010000000
VERSION     DAWCYS010000417.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3651_length_1515_cov_6.302055"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          325 a          435 c          445 g          310 t
ORIGIN      
        1 caaggatttc atcagcgatt tgaaaatcgt accggagaaa gtcgtcgtca gcggggcgcg
       61 ccgcctcatc cagcacgtca gccaggcagt cgtagatatc ccggtcaatc agcgcaatgg
      121 cgactttgct attatggcgc cgattcattt gtatcaggct gatggcacgc ctgtcgaagg
      181 actggaaatg acgccctggc agagcaatgt caaagtggcc attgcccatg atgccgtggc
      241 caagacggtg cctctcaacc tgaacgctac gggccagacg gcctggaaga ccgtcacagt
      301 ccagccgatg tcggtccagg tccgcggtga agccgatacg ctgaagaata tcagctccat
      361 cgatttgcca gatatcgatg tggaaaacat gacggaagaa aagacctgga aagtgctgat
      421 tccgcccatt gatggtgtcg tcatagaccc tgatgaagtc gacgccacac tgcatattaa
      481 gaaagatgag tagaaaggga gaagattgtg cttagagtat tgaatatccg cacggctgtg
      541 cccatgaaga agcctttgga agcgttagtt gccaagaagc tccgctgcag ccgcagcgat
      601 atttcccgcc ttatcattgt ccgccggtcc atcgacgccc ggcggaaacc gcggatttat
      661 ttcgttttta cggtcgatgt ggaattcgtc gacgaaagcc gcgtttggaa gcgctgtcgt
      721 gacaataagg atgtccgcca gctggctgag tcgccggcgc cggatatccg gcccggtacg
      781 cagccgctta cccaccggcc catcgtcatc gggaccggcc cggctggcct ggcggcagcc
      841 ctggccctgg ccgaacacgg ctatgcgcct atcgtctttg aacgggggtg tgacgtcgat
      901 acccgcacga aagacgttga agatttttgg cgcggcggcc ccttcaaggc atcgtcgaac
      961 gtccagttcg gcgaaggcgg agcggggacc ttttccgatg ggaaattgac gacccgcgtc
     1021 aatcatccgc tgctgcggcg cattttgcag actttcgtcg atgccggagc gccggaagac
     1081 atcctgtatg cctataatcc ccacgtcggg accgatgtcc tgcgccgcgt cgtcaagaat
     1141 atgcgccaca ccatcgaggc gcagggcggc gaagtccatt tttccagctg tgtcacggca
     1201 atccatcgcg acgatagcgg acgcatcacg tcggtgaccg tcaacggctg cgatgagtac
     1261 ccggcagagc tggtcgtcat gggtatcggc cacagtgccc gcgatacgta ttacatgctc
     1321 catgaccagg gcattaccct ggaaaagaag ccttttgccg tcggtgtgcg tatcgaacat
     1381 tcccaggatg tcatcgaccg ggcccaatat ggctgcccgg cagccgacct cggcctggaa
     1441 ccggccgatt acgccctggt ctatcagccc ggaaccgcgg tcgtgctagt ctatctgcat
     1501 gtgtcccgga ggtgt
//
LOCUS       DAWCYS010000418         1514 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3653_length_1514_cov_5.417409, whole genome shotgun sequence.
ACCESSION   DAWCYS010000418 DAWCYS010000000
VERSION     DAWCYS010000418.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3653_length_1514_cov_5.417409"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          349 a          480 c          373 g          312 t
ORIGIN      
        1 ccattcctta cagtcatgac ctgacgggct gaaaaggcac gtggtcgccc ctacatctct
       61 cacatgctaa cctacctttt ctctatattt caactgctcc tgcaaacggc aaatcaacct
      121 ctccggcctt tcaggccacc tcccctatga ggggaggcga actgcgacct gcgacctgcg
      181 ggccgccgta ggcggcccct acctacaata caaacaaata aggggtccca tatcggccgg
      241 cgacgccgtg ccgcggcgga tatggagctg gagcgtaaaa atttggcaaa tacaactttc
      301 caactcttct attttccaat aagatgccac tttttgcaag tggtataaga catattttac
      361 gctgcggccc ttattgatgg tctgcaggat ctgttcgcac tggtgctggc cggcatgggc
      421 ccggcgcaat tccttataag ccaggagcag gcgcaggcgc gacaacatat agcccgtatt
      481 cttcaaaaaa gcatcttgcc ggctgaacag gccttccaaa aaaggcaggg tcttttcccc
      541 attgcgccgc aaaaattcat ccataaaggt gaagagattc ttttccatcg tcccggcaaa
      601 gaggtcctgc aaatcggcca catcgacggt tttcctgctg tcgggcacgg tgatgcacaa
      661 tttctccagc tccgaaaaga catagagcaa cgaaatatca ccccaggtct ggaacagggc
      721 ccgcaaatag gcgacggccc ggctggtcag gcgcttgccc ttccctttca aatagtccgt
      781 cacataaggc atgaccgtct tttccgtcac cgcttcggcc gccacgacgt cggccatggg
      841 cttcagggcc ttgaaaaaag cactgcccgt atccatcttg cccttgctat agaacaaaag
      901 actgttctcg tcgctgagcc gccgggcctc gtccaggaac cactgctcat ccttggtgag
      961 cttgctccgc gaccggccgc cgcgcttgag cggcagaaag ggacagtcat accaaatcgt
     1021 gaccgtccca ctgctaaaga gactcgaccc ctggaaggat tcgacgaccg tcgccgccac
     1081 agcatccttc tggaaagact gaatctcggc ttccccgccg gcccgctcgt gggccgccgc
     1141 caggaaccgc ttccgactct cttccatgac atacggttct gtcccataaa taatcattat
     1201 atgtttctta tattccatga ctgttcctca ttaaacgtag aaatgcgcca ctgcccccgc
     1261 cagcggtaca gacggacctg gccgtcccgg gatggcgaaa aacaaggcac accgtaaccg
     1321 gccgccgtct cgccccactc tgtcagggtt tcatcgctgc ggcgcgagcc atagacaatg
     1381 gtcgccatgg ccgatacggg gaaatcccgg ccattccagc ccgtcaggga ccggaattcc
     1441 agacaaagcg gtactgccgg aaaaggactg gccgccgacg ccagacagcg aaaaggcaca
     1501 tcgccgctgc ccgt
//
LOCUS       DAWCYS010000419         1511 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3668_length_1511_cov_6.290522, whole genome shotgun sequence.
ACCESSION   DAWCYS010000419 DAWCYS010000000
VERSION     DAWCYS010000419.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_1511_cov_6.290522"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          351 a          390 c          443 g          327 t
ORIGIN      
        1 ttcatattcc atggggtcgc tgaggtggat gcggtcgaca ccggccagtt cctcttcgac
       61 gactttgcgc accagcggat tacggtggac aggaaagacg atttccgtat cgggaatctc
      121 ttcgataatg cggcgcaggg ccttatagat atggcgcatc ggttcaccca ggttttcccg
      181 gcggtgcgtc gtcaggagga tgacccggtg gttatggaaa tcgacgtcct tcaggtcgtc
      241 gccgaagtca tagtcgccgg caacggtcat catcaaggcg tcgatgaccg tattccccgt
      301 cacataaata tgagaaggat ttacattttc cttgagcaga ttggccttgg ccgtcgccgt
      361 cggtgcgaag tggatggctg cgatggcacc ggtcagcttg cggttcattt cttccgggaa
      421 gggcgaatag atgtcccctg tccgcagacc ggcttcgacg tggcctaccg ggacctgctg
      481 gtagtacgag gccagggcgc cggcaaaggt cgtcgtcgtg tcgccatgga ccaggaccag
      541 gtcgggcttt tccttggcca ggacatcttt caggcccatg agggacttgg tcgtaatgtc
      601 gtacagcgtc tgcccctggg acatgatatc caggtcatag tccggtgtaa tccggaagag
      661 atccagtacc tggtcgagca tctgacggtg ctgggccgta acggcgacga tggtctggat
      721 ccggtcggca tgtttctgca attccagtac gacaggggcc atcttgatgg cttccggccg
      781 cgtaccgaat acagtcatta ccttgatcat aataaaaaaa cctccttata agacaaccga
      841 ttccacccac tactgtttgt gggtagaatc ggtcgttgat ttgcgcaata tgccaatgcg
      901 ttttgccgaa taaatgatga cggcaagaac gaccaggaca gtcacgatac ccgtcttata
      961 gctcacgttg gctacaaaga gggctacgat gccgagaatc atgctgacaa aatacattat
     1021 taatacggct tgcttctgtg tcatgcccag agccagcagg cgatgatgca ggtgcccctt
     1081 atcaggctgg aaaatcggaa cgccgctcat cttgcggcgg atgatagcaa acgtcgtatc
     1141 gaggatcggc agacccaggg cgatgatggg tacgacgaga gccacggtgg ctgccgtctt
     1201 gaccaggcct aagacggcgg cgacggacag ggtatagccc aggagcatac tgcccgtatc
     1261 acccatgaaa atcttggccg ggttgaaatt gtactgcaag aagcccaaag ccgccccggc
     1321 catggacacg atgaccagag ccggcaggaa ctggttcagg ttataagcca ggaggaacat
     1381 cgaaatcgat gcgatgaagg cgacgccggc tgccaggccg tcaagaccat cgatgaggtt
     1441 caccgtattg gtaaagccga tgatccagaa aatcgtcacg ccaatggcaa tgatttagtg
     1501 caggacgatg a
//
LOCUS       DAWCYS010000420         1504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3695_length_1504_cov_2.431332, whole genome shotgun sequence.
ACCESSION   DAWCYS010000420 DAWCYS010000000
VERSION     DAWCYS010000420.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_1504_cov_2.431332"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          368 a          395 c          376 g          365 t
ORIGIN      
        1 aatagtgcag tgatctttcg ccttggcggc gtcgcgcgta cgcaggccgt acaatccagc
       61 ttcacagccc aggtccatga cggccgtctt gacgacatgc gggtgcaagg tatccagagc
      121 cgtattgagg aagcggaacc atttgccccc ttcatcgatg agtctgcgtg catcgtcaaa
      181 ggattccttt tcaaagaggt cgccaaacag atgctgcatg acataagata agcgtttcaa
      241 ttttttttga cggtctgccg gattccgttc ggccatgatg cggtcgacca ggatttcgac
      301 agccttcttt tcgatacgat gggttaactt gctcatacca atcatctcct acaaaattaa
      361 acaattgaaa cggcgctgtt tctatttctt tgtatagttc tattatacgc ttattttagc
      421 aaaagtcaaa aagtcaatat gaattggaaa acaaaaggct tttacttttt tgatgatata
      481 ctttataaat tacttgtata atatttattt acaccataaa agggaatttt ttatggctaa
      541 gaaagaaaat tagttcaagg ggtatgacat ttcgacacag gctgtacaca ctggtgccgg
      601 ctatgacgca gaaacgggcg ctgtccgccg gccattgcac atggccaaca gctacaagct
      661 gcccgatgat ctgtcgaagg tcaactattc atcgacggag tttctgatgt atgcccgtaa
      721 cgggaacccg aatcaagatt ggctggaaga aacagtagcg gcccttcatg gtgccgatga
      781 cgccatcgtc ctgggaagcg gcgtcgccgc ccttcatgcc ctgttctgga ccctgctcaa
      841 gacgggcgac cgcgtcgtct atccgaaagt cagttatatg gcagtctatc gcctgttcca
      901 cgaattattc aaccagaaat tcggcgtcga aacggtcatg gtcgatatga ctgacctgga
      961 agccgtcaag aaagcgatta cgccggggac gcgcctagtc catattgaaa cgccggacaa
     1021 cccgactgat ggcatttcag atatcgaagc catcgctaaa atcgctcatg aaaacgaggc
     1081 tgtcttgtcc gtcgacaata cctttgcgtc gcctctcaac cagcgaccac tggacctggg
     1141 agccgatttc gtcgtcgaat cgctgacgat atatatcaac ggccatggcg atgcccaggg
     1201 cggggccatc atttccaatg atttggcagc gatggaccgg atccgctatg aagcccaggt
     1261 caacgtcggc tctgtcatca gcccttcaat gcctggcaga ttttccgcgg tgccgtgacc
     1321 ttccccttgc ggatggaacg catcaacgca tcgacgcaga aaatcgccga atggctggaa
     1381 cagcggcaaa acgtcacctt cgtttcctat ccctgcctgg ccagcgatcc cggtcatgac
     1441 tggggcaagc cgcagatgcc gcacgggggt ggcgcgctca gttcctgtgg catcgccggc
     1501 gacg
//
LOCUS       DAWCYS010000421         1502 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3703_length_1502_cov_3.646856, whole genome shotgun sequence.
ACCESSION   DAWCYS010000421 DAWCYS010000000
VERSION     DAWCYS010000421.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3703_length_1502_cov_3.646856"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          299 a          407 c          403 g          393 t
ORIGIN      
        1 ttcatggtct atccgatatg cccgtaaagc cttggacaga tggcagcgct ttttcttcgg
       61 ccgtttcggc ggctggtagc ggaccagctg gcacggcgcg ccgctcatgc agtcccggtg
      121 ggcaggccgg tacttcccgt catggaagac gtggagatac cagccgcata cgatggcctt
      181 gtggcataag caacatttca ttcgtcttcg tcctgtgcgg ataccaaggt ctctaagatc
      241 caacgggcgt agcggaatcc gtcggcgaca ccctggtaat agcgggcctg ctggaacagc
      301 aggttcgagc ccgtatcttc ttcaccctgg gcgagattgt agaaatcgac ggacatgttc
      361 tccagcatgc tccggcggcg gtcgtagttg tcggcgagat tgccgacgaa ttgcttgagg
      421 tcagttttca tgacgggtat ccccctttcg atggagctcg ataatctggt cacccaagga
      481 gtgactggcg tcgtcccgcc gctcacgcag gctggcgacg ataaacagcc gcgggaccgg
      541 tgacgccttt gtatccgtcg cgatacgata caacgctccg cccatcgctc tcaggatgcc
      601 ttcgctcggt ccgtggagcg aaataagagc ttttggtttc gggtcgccct tcggctgata
      661 tttcccggcg aggatgtaat ccacaccgtt gcgggtcatg atgtcccgga aattctcggc
      721 ggcctgccgg atttcgattt cgtccggttc cgtgttgcgg tccttttctc cggccgcggc
      781 atcgtcgatg atgccctgca cttcgtcaat gagtcttttc gtttcttcat tcatgtttct
      841 ccagctcctt ttaaatcaca tgatccaatc cttggcctgc cgcaggatgt cgtagatcat
      901 cgccgggacg ttgtccaaag cgatgttcac gtcgcgggtg cattggcttt cgatgtccgt
      961 caccttgacg gtgttgccgt cgtcgctcag acggaacgag aaccacttat catcgacgcc
     1021 cagggccagt tctaagtaat gtaacgcttt ctgcttcttg actctttctt ccatagcaca
     1081 tcagctcctt aaattcttgg tacttctcgt catgctataa tagcgatgag aggaggtgaa
     1141 accatgtcta acgttttaca tttttctgat ttgtccgccc cgatgcagga taagcttatc
     1201 cattacatag gctccagata cagcaagcgt gatacgataa accatgcggt atccgcgtat
     1261 ggccttaagc agcatttcac tgcgttggcc gcatccgagg acgaacatgt aacttcgcag
     1321 tgcttcaaag aagcgatgga agcttgcggt ttccgtgctg tgcttcggga aggcacagcc
     1381 ggtgaatccg cgaattggga attcaatgcc tacgttttaa aacatccgcg agactagcgg
     1441 taatctcact tcttgaaaat gaatccgtat acagcggatc gggtaggggt gcatttttca
     1501 tg
//
LOCUS       DAWCYS010000422         1501 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3706_length_1501_cov_5.054633, whole genome shotgun sequence.
ACCESSION   DAWCYS010000422 DAWCYS010000000
VERSION     DAWCYS010000422.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3706_length_1501_cov_5.054633"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          418 a          392 c          403 g          288 t
ORIGIN      
        1 gcagcgtcga ccagattttt cagtcgacgg ccaagttact ggccaggatc actcacaagg
       61 ccaccctggt catggcgtca cagcggctca attccaagct gaagtacatc cgcttcttac
      121 cgctcgatga ccgccgggcc atcatgatcg tcgtcaccga tacgggacag gtagagaact
      181 gcatttatcc caaaccggcc ggtgccagcc tggacgatct gaacatcatc gcccagcggc
      241 tgaccaacta cctggccggc atggccatgg accatatcga tgaaaaggcc atcgaagcct
      301 ttcacgagac catcgtcgac gacgtcgaat tgtaccgcct ggccttccgg gccatgggac
      361 gggccttcca caaagaccag cagttctaca aaggcgggac catggagctc ctgaacaagc
      421 cggagttcaa agacgtcgac aaggccaaga gcctctttac catgctggaa gaacaggatg
      481 tcgtcgccaa tctccttcac gacgagggcg aaggcggggc cgtgacggtc cgcatcggcg
      541 aagaagcgaa attgtcgtct atcaatgatt gcagcatcat tgaagcgacc tttaccgacc
      601 atgatgtagt cctcggcaag ctggccgtct tgggaccggc tcgtatggag tatgccaaga
      661 tcatcggtct tttggacttt atgaaacagc atgtgacaca tatgctggca cattaccacg
      721 acgataagta gggaggggta cacagacatg agtaagaaag aaaataaaga agaaaaagat
      781 accgtattag cagaagacgc ggaaaaagtc gccgaagatg ctgctaagga aaaggtagac
      841 gaagccaagg gaacggcggc agatgaaggc gccgataaaa aggcaccgga tgccaaagac
      901 gctgctgcca aagcggaagc cgacaaggcc gccaagctcc agcagcagtt cgtccggctc
      961 caggcagatt ttgctaacta taagaaacgc acagcggctg aaaagctcca gatttcagaa
     1021 gtcgtcaaga tggacgtcat cactaacatc cttccggtca tcgacaattt cgaacgggcc
     1081 ctgcaggtcc cgcaggataa ggtggccgat gatgtgaaat ccttcatcga cggctatgaa
     1141 atgatttaca aacagctcct gggcgtcctg gaaaaggaag gggtcactaa gatcgatgct
     1201 gtcggcaaac ctttcgaccc caattatcat caggccgtca tgcgcgtgcc cagcgacgaa
     1261 tacgatgacg acgtcgtcgt agaagtcctc caggaaggat atctcctggg cgacaagacg
     1321 ctgcgtccgg ccatggtcaa agttgcattc aacggttaat catcgtttga aataagattg
     1381 gaggaatctt ttatgagtaa agttattggt attgacttag gtacaacaaa ctccgtagta
     1441 gctgtcatgg aaggcggcga acctacggtc atcacgaata cggaaggatc ccgcctcaca
     1501 c
//
LOCUS       DAWCYS010000423         1500 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            NODE_3709_length_1500_cov_4.355017, whole genome shotgun sequence.
ACCESSION   DAWCYS010000423 DAWCYS010000000
VERSION     DAWCYS010000423.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_1500_cov_4.355017"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          337 a          376 c          437 g          350 t
ORIGIN      
        1 gcccaggtcc atgtcggcat tgtcgatgac ctggcggact tcagaaacgg tgacggcttt
       61 attgaactgc atatcgagga tcgtgccgcc cgtgtagtcg atgccgaagt tgaagccgtt
      121 gatgaagata gagatcaagc tggcaatgac gaggacagac gataaggtga accaccatac
      181 gcgatgtttg acaatatcaa aactcatttc tgtcccccct ttttcaacat atgttcaggc
      241 ggtttgcggt tcaggccgta gaaccaggga ttggtgatga ggcctgtatt gatgaaccac
      301 gtgagcaggg accggctgac aacgatggcc gtgaacatgc tgaccacaag gcccaaggcc
      361 aggttgacgg caaagccttt gaccgtgccg ctgcccatga cgatgaggac gatggcggcg
      421 atcatgaccg acacgttggc atcgaggatg gtattgaagg cccggcggaa accggcttcc
      481 atagacgtcc tcaggctctt gccgacgagg acttcttcct tgaaccgttc aaagataagg
      541 atgttggcat cgacggcgaa acccatggac aagatgatac cggcaatgcc cggcagggtc
      601 agtgtcgcgt cgaggtattt caggatgccc agcaggatga ggacgtagac cagcagggcg
      661 atgttggcta cgacgccgga catacggtaa atgacgatca agaagatcat gatcaggatg
      721 atgccgatgg caaaggcctt ttcactcttg atcttggagt cgaggcccag gctcgggccg
      781 atggtgcgga cttccatgac tttcaatttg acaggcaggg caccggaacg caggaggata
      841 gccaggttct tggcgtcttc cagagtctgg ctgcccgtga tgacagcttt gccgccggta
      901 atcggttcgt tgacgacagg attggtgagc aagtcgccgt cgaggtagat gccgatttta
      961 tggcctacat tggacgctgt cagatcggcg aatttcttcg ccccttcgtc ggtaaattca
     1021 atggcgacga cattcttatg gccctggtca atctgttcct tggcagtctt cagatcattc
     1081 ccggtcatac gggtcgtgcc gctttcatcc ttgaactcca tgacagccgt tttcccgatt
     1141 gtctcgatgg ctttttccgg attcttttca ccgggcagct cgacgatgat acggcgggcg
     1201 ccttccttct ggagaagcgg ttcggtcagg cccatttcat tgatacggcg ttcgacgatg
     1261 gtgacggcac ggctcaagga gtcttccgtg accggagcgt ccggcgtatc ttcggcttcc
     1321 aagacgatat gcgtaccgcc ctgtaagtcc aggccctgtt tgatggagtt ggcaagcgga
     1381 taaatcaagg taacaaaggc ggccaggatg atgatgactg ccccgaaaaa ttttatcatg
     1441 ttccgagagc gcaacaaaac tccccgctct gggtaatctt caatagtttg ttatggatgg
//
LOCUS       DAWCYS010000424         1496 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_12535_length_1496_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000424 DAWCYS010000000
VERSION     DAWCYS010000424.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12535_length_1496_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          270 a          438 c          487 g          301 t
ORIGIN      
        1 acacaggaat cctcctcttt tttcctcttg gatgacgccg ccgcccagga cgcgttcgcc
       61 tgtttcggcg tcgtaaagta ccagggcctg gccggccgtg acggcgcgct gggcatcgtc
      121 gaattcgacc cgcaggctgt cgccgtcggg atagaccgtg caggccactt cacgggcagc
      181 atagcgtatc ttgcccaggg cccggaacgg tgcggacggt gcctgttcga cccacgtcgc
      241 ttcaccggca tggacagtct tcgagaacaa atcctgttcc gagccgacga cgacccggtt
      301 atgggccacg tcgagggccg tcacataata cggaccgccg ggaccgccga ggttcaaccc
      361 ccgccgctga ccgatggtat aggcggcaat gcccttgtgg cggccgatga cgcggccgct
      421 ctggtcgacg aagtcgccgg gcttgaagat gcccttgcgt tcgtgctgca agaagccctt
      481 atagtcgttg tccgggatga agcagatatc ctggctttca ggcttgttga agaccggcag
      541 gccccattct ttagccagtt cccgcgtatg gaccttttcc aggccggcca tggggaataa
      601 gagatggggc agcaggtcct gtgtcagctg atagaggaca taggactggt ccttgcggcg
      661 gtctgtgccg cggagcaggg aatagattcc cgtcccttcg tccttgacga tgcgggcgta
      721 atggcctgtc gccaggaagt cggcaccgaa ctgcttggcc ttttcccaga gcagaccgaa
      781 tttaatgaat ttattgcagg caatgcaggg attgggcgtc ctgccgtgga cgtaggcatc
      841 gacgaagtag tcgatgacgt cgcgccggaa gatatcgcgg aaatccaggg tataatgggg
      901 aatccccagg accgaggcga cggctttggc atcgtcgacg gaactgaggg agcagcaggc
      961 gtggacgttg tcgatggtgc aggccgggtc ttcttcccac aggcgcaggg taatgccgat
     1021 gacgtcatac ccctgctgct tcaaaaggcc ggccgtgacc gaactgtcga cgccgccgct
     1081 catagctacg gcaactcgtt tcatagtgac tctcctctgc ttttacggac agcgtcgccg
     1141 atgatggcga tggctgcttc gatttcatct aaggtatttt ctttgcctac ggacaggcgg
     1201 atgctgctgc gcagccattt gtcctcaacg cccatggcct ggaggacgtg ggacgcttcg
     1261 acggcgccgg cttcgcaggc cgaaccagcc gatacggcca gaccctgcag gtcgagggcg
     1321 ataagcagga tggcgctgtc cacgccgtcg acgctgaagt cgatgatgtt gggcaggacg
     1381 tggttgcgcc ggccgttgag gtggaagcgg gcgtcgccct tgatgagccg gtcatggacg
     1441 gcatcggtca ggcgccgcgc cgcccggacg cggtcgtccc gttcggcctc caggag
//
LOCUS       DAWCYS010000425         1487 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_13179_length_1487_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000425 DAWCYS010000000
VERSION     DAWCYS010000425.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13179_length_1487_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          293 a          470 c          421 g          303 t
ORIGIN      
        1 catatctatt caggcaaata gggaagacgc agttcgcgaa aaatagcccc gttcgggaaa
       61 tttttgcgag cgagggttac tcgaagtgat gttgtccgtt tgaaactgtt gccgcaccgc
      121 ggcatctgag tccgagcgga ctgcgacctg cgggcgccca tgaaaggaga cataccatgc
      181 acgtatatat tatcggcaca gggcccggca atccggcttt gttgacgggg caggccaaag
      241 cggccataga ggcttcgccc atcctcgtcg gcgacaagcg gatgctggcg ccttttgcgg
      301 cttccgggaa gcgcctggtc gcgacgtacc ggcgtgacga tatttgtcag cttgccgctt
      361 ctttgtccga tgaggaaggg cccatggcca tcctcgtgtc cggcgacgtc ggctttttca
      421 gcctggccgc cttgctcacg gacatccctg gctgcacggt gacccgcatc gccggtatca
      481 gcagcctcgt ctactttgcg gccgccttgc agacgacgtg gaacgacgcc tttatcgtca
      541 gccgccatgg ccgccgggaa tccctcgtcg ccgccgtccg ccagcatcgg acagtcttcg
      601 tcctgacagg cgggtccgat tcaccggccg ccttgtgccg ggaactctgc caggccggcc
      661 tggatatggt gtccgtagcc attggctgcg acttatctta tgacaatgaa aacatcctcc
      721 gcgggacggc tgccgatttc accgaccgtc ccgccgattc cctggccgtc atgatggtag
      781 aaaacgaaaa ggcccggccc tgggtgcggc ccgtccacgg tctgcccgat gaagccttcc
      841 tgcgcggcaa agcgcccatg accaagcagg aaatccgcag cgtcgtcctg tccaaactgg
      901 ctccggaagc agacgccgtc gtctgtgaca tcggggccgg tactggttcg tgcacggtcg
      961 aactggcctt gcaggctcct tttggccacg tctatgcctt tgaaatcaat gaagaggccc
     1021 tgactgtatt gcagcagaat atcggccatt tcggcctgac taacgtcacc gtcgtcgccg
     1081 gcgatgccag ccagaccttg aaaaccttgg acatcgcacc ggattacgcc ttcattggcg
     1141 ggaccaaagg caatctggca tccatcctcg atgacctgta tgacaagaat gaagcctgcc
     1201 gcatggtcat tacggccatc agcctggaaa ccctggccgc cgtcaccgct tattacgccg
     1261 cccgtaacgg ataccagctg gacatcaccc aggtcagcgc ggcccgcagc aaacgagtcg
     1321 gccagcatca tctcatgatg gcccagaacc cggtatatat catgacagct gtccgtcagg
     1381 acgcagggga agcttagacc cagactgtcg cacaaaggtt tctgccacct tgcaaggggc
     1441 ctttgctgga agaactcctc agtcggctat gctgccagct ccccttc
//
LOCUS       DAWCYS010000426         1448 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_1651_length_1448_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000426 DAWCYS010000000
VERSION     DAWCYS010000426.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651_length_1448_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          287 a          384 c          446 g          331 t
ORIGIN      
        1 gatttgaggt cagctggttt atagtccttg aggatctttt cttcgacttc tttctgggca
       61 tcgccagtaa acatcatgga gaactggcca taggtcatct tgcagacgat ggaattgtca
      121 ttggtcaggc cgctttcaga aacgctctgg gtattttctt tagtgaaagg cgtgcccggc
      181 gacaggacgg taaaggtgac gtcatcaccc agggtgaagg tatcgccctt tttcaaggtc
      241 ttataagcaa tattcttggc cttgatgttc ttcaggtaat tgcggtacat ggccgtattg
      301 gcactctggc cattgtcata gatctgcttg attttgaagt ccttgaacag ttccgccatg
      361 ccgcccaggt ggtcgccgtg aggatgggtg atgacgatga tgtcgatgtc cttgaccttg
      421 cgttctttga gctgtttgac caaatcggcc cggctgtcga cttcgcctgt atcgatgagc
      481 atattcttgc ctttatactg gatgagcgtc gcatcggcct gaccgacgtt gaggacggat
      541 atgcgcaggt ccttgtcaga ggcactcagg gactggacgg ccttggcccc gccagacgag
      601 ctggacgacg cgctgttgcc acagccggcc aggacgagca tgaagctcat gaccagggcc
      661 agggcggcgg taaagagttt tctttttttc aatttatttt cctccaatca agggctgctg
      721 caaaatgggc agggccaaat cttcttctaa agaagccagg acgccgggaa gggcatcggc
      781 tgtttccatg ggcgtaaagc cataggggcc gcgggctttg cggcaggcat cgccggctga
      841 tccgtggaga tagacgccgg cacaggcggc actgcacaaa tcgctcatgc cctgatgggc
      901 cgtcatggcg gcgatcatgc cggtcaggac gtcccccatg ccgccacagg ccataccggg
      961 attgcccgtg gagttgatgt agacgttccc cgtccgggcc gaaacgatga gcgtcggcgc
     1021 ccctttgagg acgaggttga ctttccattt cgaggcaaaa tgacgggctg ccgcgatggg
     1081 gtccgctttg atttccgtca tggacaggcc gctgaggcga gaaaattcgg ccaagtgcgg
     1141 cgtcatgata atctggctgc cccgcgaagc gatgagggcc ttttctccgg ccagcaggtt
     1201 gagggcatcg gcatcgagga cgacagggca atccacagcg gccaggactt tcaggagaaa
     1261 ttctttcgtc ccggcgtcgc ggcccatgcc ggggcccatg gccaggacgg accaggtctt
     1321 gctttcagca atgatttcat cggcatcagc agcagtgaag tggtcgccag taccgacgcc
     1381 gcggaccatg atttccggct gggcgccgat gcagtacggc gccgtcgcac cgggcacgcg
     1441 gaggaaga
//
LOCUS       DAWCYS010000427         1439 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_10058_length_1439_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000427 DAWCYS010000000
VERSION     DAWCYS010000427.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10058_length_1439_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          373 a          389 c          358 g          319 t
ORIGIN      
        1 ccgtcgacag gggtccaagt atattggcga actcaaaagc agcactcaag acgctatcct
       61 cgttagtttt tacaattctg aaactgacgc gtatctcctg gaagtcggcg tccaatacat
      121 ccggatttgg aaagatggtg cctatacggg catagaaatc agtacgcctt atagtaatcc
      181 taaagggtta caattcagcc agtccggcga tgtcatgtat atttgttccg gccagtatcc
      241 ggtaaaactg ctgcggcata aacaggacgg ctgggatctt atcgacatgg aaataaccga
      301 accctattat gacgccatgc tcgatgccgt cgtcgataat aaagtcacgt catccggcac
      361 ctctgggacc gtgactatca gctcgcaagc cgctattttt catagtggca tggaaggcgg
      421 ctatattcag ctcaaccaga aagtcggcag ccagaccttg tccggatcct ggggcgaaga
      481 aacgacgacc tggacgtcag gcgaactcta tgtcggggaa aaatggaaaa tcgtcactca
      541 cgggacacac cactacgaaa tacttctgca gaagcgggaa aagaaaagct ccatttggcg
      601 ggaatatcgc aagtacacgt ccaatgacga ccagaactat accgaatcgg gcagtgaaac
      661 ggagggctgc tatttgcggc tcatcgtcaa agtctggaat gatgatgagg caagctcatc
      721 gaaactgacc gtagatctga cacgcttgcc ctatacccat acggggacgg caaagattac
      781 ggctgtcaat tctgggacga ccgtcaccgc tgccgtcaaa gatgtattcg gcagcaccga
      841 cgaaacggcc gactatgcat tatcgtcgtg gaacagctat tacggtttcc cgcagcagtc
      901 ctgttttttc caggaccgct tagtctttgc cgctaactac aagaatccct attcgctatg
      961 gatgagtaag accggcgagt atccgaattt ctccgtcgaa aaagtagacg gtacggtcac
     1021 cgatgacagc gctatcaaaa tggacctcat cgtccgcagc tcctatcaga ttcgccacct
     1081 cgtcccgtcg caggacctcg tcgtcctcac gtcagggaat gaatgggtca tcagtggcga
     1141 cagcgtcctg acgccgacga aagcctaccc aaagtcacag accatgcgcg gctcctcgac
     1201 gtgtctgcca cagcatatcg gcaaccgtat cgtccatgtc cagcggtccg gttcgaccgt
     1261 ccgcgacctg ggttatcagt acgaatcgga taactataac ggcgacgagc tggatattct
     1321 ggctacgcac ttagtcaaga accataaact gctctcatcg gcttattgcc aggaaccgga
     1381 ttctacgctc tttttcgtcc gcgatgacgg cgtactcctg gcctttacga tgatacgag
//
LOCUS       DAWCYS010000428         1423 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_8767_length_1423_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000428 DAWCYS010000000
VERSION     DAWCYS010000428.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8767_length_1423_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          233 a          525 c          376 g          289 t
ORIGIN      
        1 acgccatcgc cggcctgccc gatgccgcag gctacatcat ttcggccatc gtcggcacag
       61 ccctgctcat catcatcttc aaagtcatcg cgtccttcct gccatcccgg ccgaccttcg
      121 atacgccggc ccagcactag ggaggattgc ccatgaagct ccctgaatgg gcggcccgcg
      181 aggaaatata taatcccggc agcgaccggg attatttcat ttcccgctcc ctgctccgcc
      241 tcgtatccat cctgacggcc ctgcgcagcc agggcctgcg gccaacccgt cccatcggcg
      301 cggcgccggc cttattggca accctggtca tggtcctctt cgtcgtcctg gcccggacga
      361 cggccttcct ctgggccgtt ctggcaggcg aactggtcct cctctgcttc catcgcggcc
      421 ggcagctgcg ccaagtcctc ggcgccgccc tggcagccgc tctgttctgc gccgtcctcg
      481 tcgccccgtc ttttttcctg ggcaatggcc gctacgcctt tctcctgccc tgcaagactt
      541 tcctcaccgt gacggccctg atgctcctgc aggaaaacct ggcctggcat gaactgaccg
      601 gcgccctgcg gacgttccgc gtcccgtcgc tggtcatctt catcctggac acgacgctgc
      661 gctacatcgt catcttaggc caggaagcgt ccgacctgct gacggccctc aagctgcgct
      721 ctgtcggtca taatcccgac aagaaggccg ccatgagcgg cgtctccggc attttgctac
      781 aaaagtccca gcgcctgtcc caagacctgt acgaagccat gtgctgccgc tgcttcactg
      841 gcgattaccg gccctatgag gacggccgga agcgccgttt ctctcccgcc acggggctgc
      901 tcgtcctggc cctgctggcc tatgtctatc tcttcatccg cctggaagga gtcctctcat
      961 gatacaactc gacgacgtct gtttcgccta tgacgacagg cccatcctct ctcatctcac
     1021 ggaaaccatc gaaccgggcc aggccgtcct cctcagcggt cccaacggct cggggaaatc
     1081 gacgctcctg cgcctgctga acggcctgat tttcccccag caggggacgt atacctttga
     1141 cggcacggtc atcacggccg gcaagatgcg ggaccacgcc tattccaaat ggttccacca
     1201 gcgcctgggc tacgtctggc agaatcccga ttcccagctc ttctgcagca gcgtccgcga
     1261 ggaactggcc ttcggcccga tccagatggg attgaagcct gaagaaatcc accagcgcgt
     1321 cgacgatgcc ctggaactgc tgggcctgac ccggctggcc tcccgcccgc cttacaccct
     1381 gtccggcggc gaaaagaaac ggacggccat cgcctccata ctg
//
LOCUS       DAWCYS010000429         1389 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_510_length_1389_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000429 DAWCYS010000000
VERSION     DAWCYS010000429.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510_length_1389_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          354 a          391 c          362 g          282 t
ORIGIN      
        1 aaaaggagta gtaatgctat gaatgattcc ggattgctca tcgtcgtgtc cggcccgtcc
       61 ggagccggca aggggactat ctgttctgct ctgcgggaac agttccccaa tatccagtat
      121 tccatttcta tgacgacccg tgacccccgg cccggggaag tagacggcgt caattattat
      181 tttgccgata acgcgcggtt cgaacagctc ctggcagaag atgccttcct ggaacacgcc
      241 aaggtctacg accattacta cggcacgccc aagaagtacg tctaccagat gttggaagaa
      301 ggcaagcatg tcatgctgga aatcgacatc cagggagcca tgcaggtcaa agaacggtat
      361 ccccagggcg tcttcatcta catcgtacca cccagctggg ctgtgttgga aaaacgcctc
      421 cgcggccgcc agaccgattc ggacgacgtc atcgccggcc gcctggccaa ggcccgggaa
      481 gaactggatt ggatcgaccg ctatgattat gtcatcgtca acgacgattt ggataaggcc
      541 gtcgacgaag cggcttctat cttgaaagcc gaacagtgta aagtgacgcg caatgcccat
      601 cgcatcgccc atacgaaaga attgtacaaa taataggaga gatgaactat gttaattaaa
      661 ccgactcttg aatccctgat gactaaagtg gacagcaaat atacgctggt tacgctggct
      721 gccaaacggg cccgccagct taccgatggc gacgaacccc tcgtcgacgt cgatacgacg
      781 aaagtagttt ccattgccat ggaagaaatc gaccagggca agatcacgta tgaagcaccg
      841 cgtgacggca ttaaataaca cggaggtgtt ttccatgagc ttggaaaaga aaaagattgt
      901 cttaggaatc actggcggca ttgcggcctt caaggcagct tctattgcca gtcagctccg
      961 caagcggggg gccgaagtca aatgcatcat gaccgaccat gcgacgaaac tgattacgcc
     1021 cttgacgatg cgggaaattt ccggcaatcc cgttgccgta tccatgttcg acgatgtgcc
     1081 tgaattcaac gtagaacaca tcgccctggc ccgctgggcc gacgtcttcg tcatcgcacc
     1141 ggcgacggcc gacatcattg gcaagatcgc caacggcatt gccgacgaca tgctgtcgac
     1201 gacggtcatg gctacgacgg caccggtcct catcgcaccg gctatgaata ccaacatgta
     1261 tctcaacccc attactcagg acaatatggc caagctgaag aaattcggct atcatttcat
     1321 cgctcccgac agcggtcatc tggcctgcgg catcgacggc gtcggccgcc tgcccgaacc
     1381 ggataccat
//
LOCUS       DAWCYS010000430         1380 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_2364_length_1380_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000430 DAWCYS010000000
VERSION     DAWCYS010000430.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364_length_1380_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          288 a          425 c          362 g          305 t
ORIGIN      
        1 atcaagatgt ccctcttgcg caacgtcttc ctggccggcg tcttcctggc gcccgtaggc
       61 cggcatttcc tcaaggatta ccagaagtcc cgtattacgg tcttcctcaa tcccaatgcc
      121 gacccctttg gcgccggcta tcatatcatc cagtcgaaga tcgccatcgg ttcgggcatg
      181 ctctttggca aaggcctctt ccaggggacc cagagccagc tgaacttcct gccggaaaac
      241 catacggact tcatcttttc cgtcatcggc gaagaactgg gcttcctcgg ctgcgtcttc
      301 ctcttgttcc tgtacttcat cctcgtctat cgcggcctca tgatcgccaa ggactgcaag
      361 gacagcttcg gcatgatgct gttgacagga gtcgtttcca tgtgggcttt ccagatcctg
      421 gtcaacgtcg gcatgacctg cggcatcatg ccggtcacgg ggatccctct gccgttcatg
      481 agctacggcg tcagcgcctt gacgaccaat atgatgggcc tgggcctctt actgagtatt
      541 tacatgcgtc agcagacgat gattttttaa cccgccgtga aggaggaaat taatttgtca
      601 catcccgtgg agatcgatcc gattttactg agcaaagtca gcaagccggc ccgctacgtc
      661 ggcggcgaat ggaacagcgt cgtcaaggac cacgatgccg tcaaactgac cgttgcctat
      721 tgtttccctg acgtctacga agtcgccatg agccatctgg gcctgcgcat cctctatgcg
      781 ctcctcaatg aacggcccga tgtggcagcc gaacgggtct acgcgccctg gccggatatg
      841 gaagaagtca tgcgcagcca gggctatccg ctcttttccc tggaaacgaa gacgcctgtg
      901 cgcgacttcg atatggtcgg ctttacgctc cagtacgaaa tgagctttac caacatcctc
      961 aacatgctcg atttggccgg catcccgctc tttgccaaag accgcggcga cgacatgccc
     1021 ctggtggcag ccggcggtcc ctgtgcctac aacgccgaac ccctggccga ttacatcgac
     1081 gtctttttcc tgggcgaatc ggaagaatcg acgcaggtcc tggccgatac gatcatggcc
     1141 tggaaagagc agggaaaacc gggcggcaag aagggcctct tgaagcagct ctcccagcag
     1201 ccgggcaact acgtgccgtc tttctatgaa gcccagtacg atgcaaaggg cgacttccag
     1261 ggcctgaccg tcactgaacc gggagcgccg gcagaaatcg agaaatgcgt cgtttccgat
     1321 gtggaacacg tcttcttccc gacgaaaccg atcgtaccca acatcgacat cgtccacaac
//
LOCUS       DAWCYS010000431         1380 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_10142_length_1380_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000431 DAWCYS010000000
VERSION     DAWCYS010000431.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10142_length_1380_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          330 a          332 c          380 g          338 t
ORIGIN      
        1 ggatagtcat gaatctgacc ttgttaggga cggggaacgc aacggtgacc cattgttata
       61 atacttgttt cgtcctccat gaaagcggtc aatatctgct cgtcgatggc ggcggcggga
      121 acggactcct gtgtcagctg gaaaaggccc atttgcgctg gcaggatatg cggacggtct
      181 tcgtcaccca taaacacatg gatcatcttc tgggcatact ctggcttatg cgcaggatgt
      241 ggtagtcctg gcggcgcggg acctatgagg gcgaagccgt tatctacggt catgatgaag
      301 tcatttccct gctgcgcacg atgggaaagg ctctgctgtc ctcgaaagac agtgctttcc
      361 tcgatgaccg ccttcatctt gtgacggtga ccgatgggga gacgcgtcag ctcatgggtc
      421 atgacgtaat ttttttcgat attcgttcga ccaaggccaa acaattcggc ttttctttag
      481 aactcgccga cggcagactg acctgctgtg gtgatgagcc gtatcagccc tgggaagaag
      541 cctatgtccg ggatagtaaa tggctccttc atgaagcttt ctgcctcgat gccgaagcgg
      601 atatcttcca tccctatgag aaacaccatt cgaccgtcaa agacgcctgc caaacgtcag
      661 cccgcctggg cgtacagaat ctgatcctct accatactga agacaaggac ctggcccatc
      721 gccgccagcg ttaccatgcc gaaggaagcc ggtattatca gggaaacctg tatatcccgg
      781 acgatttgga cgtcatcgct ttatagtttg tagtacggat ttgtaggagg cgcacgtggg
      841 caatccgttc gggatttgga tggtttttct agcaaactgg tcggggaatc gtacatatcc
      901 cggagcgggc gccctacttg ggcgccccta cagaaaaggg cttatcgtcg gcgacaagcc
      961 cttttcgttg tacaatgtat ccgattctgt tagaatagac gtaaggccat ggcctcgaaa
     1021 gaatttggca gcttgggaaa atgttgcagc aaaaattagc agaagttgaa gaagatcatt
     1081 aagggataga gtaaggagac agggtaaaat gatacgtttt aacaatgact ataatcatgg
     1141 cgctcatgcc agcgtcttgc aggctttgaa tcagacgaac gatacgcagt acggcggcta
     1201 tggtgtcgat gactggtgtg acaaggcaga aaagctgatt ttggaattgg cccagtgccc
     1261 gcaagggaag gtttactttt tcccgggagc gacgcaggcc aatttcgtca ccatttcagc
     1321 cctccttggt tctgtgacag cgtcatctgt gccgacacgg gccatatcaa ctgtcatgaa
//
LOCUS       DAWCYS010000432         1371 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_10041_length_1371_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000432 DAWCYS010000000
VERSION     DAWCYS010000432.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10041_length_1371_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          392 a          332 c          292 g          355 t
ORIGIN      
        1 gaataattct acacgtcagg cattaattcc tgctttcccc tgcctttctt cggaatatat
       61 ttcatcgtgt acgtaagacc tgtctggccg cccatttcga tgaaatgcca gtttttcttc
      121 catctgtcct atttaattca acgaaatcca tgtgttataa tagagacaaa ttttactata
      181 tttgaggtga ttatgttgaa aggaaaaaaa ctcgcatcca tgttagctgt cacagctatg
      241 ttgaccagct tcggcctgac ggggattgtt gaaggtgcca gccgggccga attagccgcg
      301 attcacgtca atcagtccga aaatttccag tattggaacg agaattctcc ggctaagaaa
      361 aagctggtag cttatgtgca aaaagtaacg aatcccaaaa atcctgattt catcccggca
      421 gaacatcgcc tggccgtctt cgatatggat ggaaccctgt tgggagaacg gaccccgtcc
      481 tatacagatg gcgtctttta tgcggaccgt gtcctgcatg acccaaactg taaagtgtcc
      541 gatgaagaca aaaaatttgc tcaagcctat tgggatgcca tttctcagcg gaaacccgtt
      601 cccccggaaa tcgaaaagaa cttgtaccac aacttatatg ccggactggc cgggatgacg
      661 ccgcagtccc tggacgctta cgcccaggga tttatgaaaa agcccgttat cggcctgacg
      721 aacctgactt atggcgaatc cttttacctc cctatggtag aagtgatttc ctatttagaa
      781 gctaataatt tcacctgcta catcgtttcg gcctctgacc gccctctcgt ccgcgcactc
      841 gtccatggag ccctgcctat cgcaccgaat caagtcatcg ggtcggatcc gcaaatcgtt
      901 gccagccatc aaggccaaac tagcggtggt tcatatcaaa tgacacagga agataccatc
      961 cagtacggcg aattggaccg ggtcaatgaa aaaatgaata aagtgtcatc cattgtccgt
     1021 gaaatcggga tacagccggt cttagccttc ggaaacagca gcggcgatta ttccatgttc
     1081 cggtatacgc taaaggacaa cccctataaa gctgaagtat tcaatattct ctgtgatgat
     1141 acgcaacgtg aatatggccg ccctgaacgg gccgaacgcg ataagaagac atctgccgaa
     1201 aatggctgga atactatttc catgaaaaat gactggaaga caatttacgg agataacgta
     1261 aaaaagacac aatgacccac cagtccatct attttgttcg aaatgttccg tcatactttg
     1321 aataataatt ctaaaagcat tgacaaatac tcgttttaat gtataatatt c
//
LOCUS       DAWCYS010000433         1368 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_7987_length_1368_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000433 DAWCYS010000000
VERSION     DAWCYS010000433.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7987_length_1368_cov_7.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          313 a          347 c          329 g          379 t
ORIGIN      
        1 tacgtagcca gttcctttac ctgttcgtcc gtaatgacct gctgtttctg cagttccggc
       61 ggaacgagtt tttcttcgca atcgccgccc ggtttgcgga ccagttcgat cggtttggta
      121 ttgatcatct tggagaggat ggtcaggtcg tctttgcgga ctttgaagtt atccggcgta
      181 accgtgccct gtacgacgta ttcgccgagg ccccaggccc cttcgatggt aatcgtcgaa
      241 tcatcgcccg tgaccaggtc gacggtgaac atgacgccgg cagctttgga gaagaccatc
      301 atctggatag cggcgctcaa agcgacggag agatgatcga agccctgttt cgtgcggtag
      361 tacgtagcgc ggtcggtaaa ggtcgatgcg taacattcct tgaccttctg gatgaccatg
      421 tcagccccct ggacgttgag gtacgtatcc tgctgaccgg caaagctggc gtccggcaag
      481 tcttcagcgg ttgcactgga gcggacggcg acgaaaggat tggcttcgcc gactttctta
      541 cccaattctt cgtaactctt gcggatatcg gctgccaagt cggccggcat ttctgcatca
      601 cagatggcct tgcggatctt tgccgtcaca tcgcggagct ggacagcgtc ttcgacgtcg
      661 gtcaggcctt tcagtaattc cgggatcttg acgtccatgc ccgtttcatg catgaaatgg
      721 cgatagcctt cggctgtcgt cgcgaaccca tagggaacgg gaacatccgt ctgagacgtc
      781 aattcaccga gggaagacga tttaccgcct acgagagcta catcggctct gcgcagttca
      841 tcgaaccaca atacaaatgc tttttctctg tcctttgcca taatccttac ctcctgctat
      901 tataaccaag cgtgtatgct aaattgtctg cttgttacgt ttatagtacg ctatttttct
      961 gcgattgtca atggcatttc taaaaaagct acacgatact cttttaaaga gtatactaca
     1021 tatgaaatat tttcggattc cgtgtagcgt aaaacgattt aatcgccaaa aagactttaa
     1081 attttttcaa cctgtccctc ctatgtcccg cctatatagg ataatgatgt atgatgtgat
     1141 ataataagaa attagtaatg tctcattacc tgctcatctt gaacgattac gtcgtcacgt
     1201 aggaggaaaa aatgtataca cccagacgca gagcaaaaaa agccaaaaaa gccggtcccc
     1261 ttcgccgcat ccgccttttc atcgccttat gccttgtcgt tttcgcaggc ttagggtttg
     1321 gctatatttt tgctgcctat caaagtttgc ctgctgtagg caataaca
//
LOCUS       DAWCYS010000434         1321 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_11796_length_1321_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000434 DAWCYS010000000
VERSION     DAWCYS010000434.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11796_length_1321_cov_3.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          319 a          335 c          439 g          228 t
ORIGIN      
        1 gcggcagggt gatgcggaag aaaatagtcc agccgctggc tcccatgagg gccgccgctt
       61 cctcttcatc gcgcccgttg gtgctgaggc cggggatgat ttcccggaag acgaagggaa
      121 aggtgacgaa aatcgtcgcc aagatgacgc cgggaacggc aaagacgatt tggatatccc
      181 tcccccagta gtcgttgagg acttcgatcc actgaccgat ccagcccatg cggccgaagg
      241 tcatgatgta ggccaggccg gcgatgaccg gcgagatgga aaagggtata tcgatgaggg
      301 tcgtcaggaa atgcttgccc cggaagtcga atttggaaat agcccaggcc gcaccgaggc
      361 cgacgatagt attgacgatg acggcggcga ccgtggcgat cagggtcagg cgcagggccg
      421 acaggacgta tggcgtcgtc agggcttgta cgtaaaagtc ccagccttcg gccagcgagt
      481 tggtgaggac cgacacgagg ggaacgatga gcatgaccac gatgaagacg acgctgatcg
      541 tgagcaaggc gatttccgtc ttactgtgtt tcataatggc tcccctcctc tactggacgc
      601 gccgggcctg atggacctgg atcatattga tagaaaacag tgtgacaaag gcgatgacca
      661 gcatggccag ggcgatggcc gtggcgctgg cgtaatcgac gtaattgagc ttctgcatga
      721 tgacgtaaga aacgacctgc gtatgatgct tggcactgtt gccagaaatg tagatgacgc
      781 tgccgtattc gccgaggccc cgggcaaaag ccaggccgaa gcccgtcaac agagccggcc
      841 gcagttccgg gaagatgatg cgccggaaaa tggtgaactg cgacgcgccg agcatgctgc
      901 ccgcttcttc atacgtcggg tcgagcttct ccaggaccgg ctggatcgag cggacgacga
      961 acgggatgcc gacgaagacc atggcgatgg tcaggccgat cttggtatag gcgatctgga
     1021 tgccatagct ggcaaagaaa ctaacgaaga agcccgtatc ggaatagagc ttggaaaggg
     1081 tgatgccggc gacagccgtc ggcaaggcaa agggcagttc gatgacgccg ttcaggagac
     1141 gtttcccgaa gaaatcgtag cggaccagga cccaggccag gatgaagcca aagacgcagt
     1201 tgaccaaagc cgccaggaca gaacaggaaa tgctggtgat gaaggcgttg cggatgccgc
     1261 tgttggtgat gagcttggca tagtcattgg ccgtcagctg cagggagtgc gccaggaccg
     1321 a
//
LOCUS       DAWCYS010000435         1318 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_3244_length_1318_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000435 DAWCYS010000000
VERSION     DAWCYS010000435.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244_length_1318_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          298 a          363 c          337 g          320 t
ORIGIN      
        1 aagccgcact gcgccccgtt tcttcatcag gcgtgaagcc gatgcggatc atcccgtgag
       61 tgatgtccgg gtgctggatg aggtattcca tagcgctgac gatagccgtg atgcccgctt
      121 tatcgtcggc cccgaggagg gtcgtcccat ccgtaaaaag gatgtcctgg cctttgtatt
      181 tcaggacttc cggaaaagcc ttcgtcgaaa agacgatgcc ccttgcttcg ttgaggacga
      241 catccttgcc gtcataatca gtgacccgga cgggcttgat gtccttcccc gatgcatcgg
      301 gactggtatc gacatgagaa atgaagccga cgaccgggcc ttctgccccg ttggccggca
      361 gcgtggccat gatatatccg tgttcatcca aggtgacatc agacaagccg attgccttca
      421 gttcctctgc caaggccttg gcaaagacca gctgtcccgg tgtactcggg cagacggcat
      481 cattgtcttc atcggactgg gtatcaaaag aaacatactt caaaaaacgc tgtacaacgt
      541 gttccgtgtc tatcatagta atcattctcc ttctttaaga atcgttaatt caattttatg
      601 ccgttatcgg caatttatca atcgtttaac ttgaatattt tcataagata aattgccgat
      661 aatcagtcac gcagccgcag gaccaggatt ttttcccggt catcgtcctc atcgaacagg
      721 tcccggcagt cgatgacgtc ttcaacgacg aggttgggat gatgcgtcag gaaagcttct
      781 gtttcttcat aaggataata gaaaaacagg cgcatcggcc gcggcgcctg gtaatgggcg
      841 tcgaaaagtt tccccaggac ccgcttcagc gtgtgcaggg caaaggggtt gaagaaaaag
      901 aaacgattcc cctgcggcgg gacctcgtac tccacggcat cggccaagac gaaagtcacg
      961 cgccgccggg acgccgccgt ctgtccattg gccagggctt tttcccacag ccgccggtcg
     1021 tattccacac cgatggcctg acagcgcgtc tgataagcca ggaagaagct gacccgtcct
     1081 ttgccgctgc cgtaatcgat gagcgtatcc ctcttgccaa tatagccgct gttggccaat
     1141 cgttcgagga cgccgtaatc cgtcggctca taaggatggt gctccccatc ggaatggctg
     1201 tcgtcgcggc cagccgtttt gatatgcaat aactgttccc attcccgttc atccatcgtt
     1261 atgccctcca tgattatatg ataccttatt atactcgctt ccaggggata gaaaaagg
//
LOCUS       DAWCYS010000436         1299 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_8223_length_1299_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000436 DAWCYS010000000
VERSION     DAWCYS010000436.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8223_length_1299_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          358 c          343 g          241 t
ORIGIN      
        1 gaattcgtgc gcggcgaaac gttaaaagaa tacatcgaaa aacacggcca cctgccgatc
       61 aacacgtcga tacagattgc cttcgatatc ggcgaagccc tggaaaatgc tcatgccaac
      121 ggcattgtcc actgcgacat caagcctcat aatatcctgg tcaccgagac aggacgcatc
      181 aaagtcgccg atttcggcat cgcccgggct gtgaattccg tcgaaaacag cgaccgtacc
      241 gtcctcggct cagtccatta tttttcaccg gaacaggcca gtggcggcca tatcgacgaa
      301 cggacggata tttattccct aggcgtcgtc atgtatgaaa tgatgactgg cgtcgtccct
      361 ttccagggcg atacggccat cagtatcgcc ttgaagcacg tccaggacga tattcccttg
      421 ccgacgaagt acaaccgccg tatcccgcgg ctcgtcgaac aggtcatcct caaggccatg
      481 gccaagaacc cggacgaccg cttccagtcc atcggcgaga tgatgtcgga actgcgcctg
      541 tcccagggtt tcatcaatac caacggcgct cggccgatca tcagcaaggg tccgtataac
      601 ttcggcacgc agaaattaca gcctatcaag gaaaaggacc tgcccgaaga agaaccgaaa
      661 gaaaagaaga agaacccctt cacgcggttc atggattcta tcagcaatca ttcgcagaaa
      721 actatcatca tcggtatgct cggcgtcttc ctcgtcgcct ttgtctgggc cttcttttcc
      781 tttgggaatt tttggagtac cgaagacatt accgtaccgg atgtgacggg taaacaggtc
      841 gaaatcgcca agaagatact gaaagataag aaactcgacg tttccgtcaa ggaagtggaa
      901 agcgacgaag tgccagtcgg cgaagtcatt tcccagacac cgtctggcgg agccgtcgtc
      961 aaggccaacc ggaccattta cctgaccgtc agcaagggga ataaaggtga agaagtcctc
     1021 ataccggacc tgcggagcct gaccctcgat gaggccgaaa agaaactgaa ggaaatcggc
     1081 ctgtctgtgg gcaaggtgcg ctacaaagag agcgctcagt atgccgacgg gaccatcatc
     1141 gaccaggacc cggaatcgcc gaagaaagtc gaaaagggga cgaaagtcga cctcgtcgtc
     1201 tgccgtaaga gcgaagccga aaaagaagca gaagcggcca agaatgaagc ccctaatacg
     1261 agcggcatga gcctcgatgc ggccgtgaag gccctggaa
//
LOCUS       DAWCYS010000437         1287 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4836_length_1287_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000437 DAWCYS010000000
VERSION     DAWCYS010000437.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4836_length_1287_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          196 a          405 c          357 g          329 t
ORIGIN      
        1 gaaaatcgac ggcgtccctg tcgatatgaa gatcatcagc cgtgtcctca gttatttctt
       61 cctcttcatg gcgacgttct tcgtctccat gctgatcatt tccctgtccg gcgtcacgcc
      121 catgcaggcc atggggatgg ccgtaggctg cctgtccagt gtcgggtcga cgatggacct
      181 cttcggcgta gccgacgttt ccgtcctgcc ggcatggact aagctgtatt gcagtttcct
      241 catgatttta ggacgcgtcg aaatcttttc cttcctcgtc gtcatccaga ccgcattcgg
      301 ttatctgcgg cggcgctggt aggaggtaca ccatgaatat acgaattgtt ttatttgtct
      361 cggggaaatt ggctcaggcc tgcgggctgg ccttgatgat tccctttgtc gtcgcccttt
      421 tctatgggga agccagtatc gccgcgttcc tcgtggccat tgtcatcagc ctcctcatag
      481 ggcgcctgct catgtggcag ggccatcggc cgacggacag cctgactgtc cgcgaaggcg
      541 tggccattac ggctctgggc tggatcatgg tcactttcct gggcatggtt ccctatgctg
      601 ccggcggcta tttgtccgtc ctggacggtg tctttgaatg tatttccggc ctgtccggca
      661 cgggggcgac ggtcatcgac gacatcgaag ccatgccggc cagcctggtc ctctggcggg
      721 ccctgaccca ctggttcggc ggcctgggta tcatcgtcat cttcatcgct gtctttcctc
      781 agttcggcaa gggcatcgtc cacatgttca atgccgaaag cacaggcccc cggtcggacc
      841 gggccctgcc gcgcatcaag gagatggcca aggccctgtt cacggtgtac gtcgtcttta
      901 ccgttgcctc gacggtcgtc ttcatgctct gcggcatgtc cttcctcatc gcagccgacc
      961 acgcctttgc cactatcgct acgggcggtt tctcgccgta caataacagc gtcgcccatt
     1021 tcgacagccc tgtcgtcgaa tgctgcctgt cctttttcat gctcatcagc agcgccaact
     1081 tcggcatgta cgtcgcggcc tggaagaagg gctgcaaagt catcctcggc gatacggagt
     1141 tccgcgtcta cttggccatc gtcgccatct cgacggcggc catggccctc aacctggtcc
     1201 tggcccagca gtgggacggc atcgaagccc tgcgccaggc ctttttccag tccgtgtcca
     1261 tttcgtcgtc gacgggcttc gtctcca
//
LOCUS       DAWCYS010000438         1264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4399_length_1264_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000438 DAWCYS010000000
VERSION     DAWCYS010000438.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399_length_1264_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          368 a          284 c          323 g          289 t
ORIGIN      
        1 cggtagaata taaatatgca ttggaagtgg ctgcggcttc tgttccgtcc cggatgggct
       61 tggatgactt gcgtatttcc gggtccggcc agttttcggc tcgccagatc gacgccctcg
      121 tccatgagct gcggaaaaag acccggggtc ctatttatga cgtcgacttg cgccaggaat
      181 cacacggctt ttttgacggc acggccgtca gctggtatgg ccgtcacgat tggggaaata
      241 taggaaagtc ccaggccgag gttctcatcg atgaacagca gcgcttgcag gccgctttgg
      301 ggacagacgt cacggtctac gaccagggga aaggcgattt gcccgttcat cctcagatta
      361 tgaccgtacg acgcgtccag acggaacggg aactggcaga aagcaagggc gtccactacg
      421 tccgcctggc cagcacggac catctctggc cgacgccggg cgaaatcgac gccttcctag
      481 ttttcgtccg cactttgcca gacgatgcct ggctccattt ccattgcgaa gccggagccg
      541 gccggacgac ggcttatatg gtcatgtacg atatgataaa gaatcccgac gtgtcctacc
      601 gggatatcgt ctatcgccag tatgaaatcg gcggtaacta cacgccacat gacgtatccc
      661 gtcccaaacg aggcgattgg aaaggaatgt attatcatga aaaacacgaa atggtgtcct
      721 tattttatca gtatgtacag gaccaggcga aacagggatg gagccagtcg tggtcacaat
      781 ggttacagaa taaaagaatg agagtcaata acgtttataa cgataacgac atataaaacg
      841 acaagaaatg tataaataac catttacaaa atttttatgc aaaaataagc tttaggaaat
      901 caaaaaataa gactaaaaaa tggaagctga atggcgctgt taagccaatc tttatttcgc
      961 taaaactggt ttgcagatat agagttttga caagcgccgg gaaaagttgt ataataatcg
     1021 caacaggtgt ggtgcggatg caggcaacac ctgcggaaag agcaaaaaaa cagtacattt
     1081 ttcaaggcag taaaagagag gaaactagga caactcgcca atgaaccctt gaaaaaatac
     1141 gtttcgacgc tcgctcgtaa cggtgtaagg cttttaacga aacttatttt ttattggaaa
     1201 aggggtaatt ctcatgaaat ccaaagtatt agcagctctc gtagcaacta tggctgttgg
     1261 cgca
//
LOCUS       DAWCYS010000439         1262 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_994_length_1262_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000439 DAWCYS010000000
VERSION     DAWCYS010000439.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994_length_1262_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          270 a          340 c          326 g          326 t
ORIGIN      
        1 gcgggctggc cgtgatgtta cgcggattgt cgctgccggc gccgacgcag tagacgccgt
       61 ggtcgaaaca ttcctgatag agggcgagga cttctttttc gcccaaatga tggagctgca
      121 gggcctcacc tgtcgtgaag tggacgaggt cgatgtcgta gcggtcgagg acgtccttca
      181 ggaaagcgat gtgtgccggt tcgatggcac cgccggaaaa gcgcaggcgc agcatggtgc
      241 tatgggcccc ccgttcgcca tagcagccga acttgcccga cgtcccctta tatttggccg
      301 gcggcagttc cttagcgtaa aaagcctgcg tcgcttcgac gaactgcggg aagcggcggc
      361 tgatttcttc ctttgtttct tttgtgattt gagtcatttc ataaacaccc cttaatttga
      421 gtcatttcat aaacacccct tatttaatcc aaaactatac tagatttgta ccctattata
      481 acagactttt ttaaagaggg caaaagattc accagaaaat ttgctttttg ttatattttt
      541 gtttaacctt agtatgtata ctagaagaaa atttagagaa accaggtgag gactatgcaa
      601 ctccgggact ggcggctgcg caagcgcctc cttttttcca actttatcat gattttcatt
      661 cccgtcctgt ttacggtcgt cgtcagcatc gccatctttt tggccctgca attcggcaac
      721 atcaaccggg ccagtatcat ttcctttatc tggcctgaga gcggtcagaa catgtccatc
      781 cagttcgagc tgagccgcct ccgtgtccgg gccgatcagt ataatggcaa catggaccgt
      841 ctcctccagg ccgccgatca tttggaggac cagggcctca atgtatccat ttggcgcgat
      901 aaggaagacc tctacgatac ggatggcatc gacaaggtgt acctgcgcca gcaagtcatg
      961 gccggtcatc gtcagggcca tgccgacttt acctggactg gcgaaggcct gcgcttttac
     1021 tacgtctcgc ctcatacgga catccatttg gccgtcgccg gtgccgtgcc gagttattcc
     1081 gaaaacgagt acatcgactt gtcatcgaag gacatcttga aggtcgcctt ttacgtcctg
     1141 gctattctga ctatcttgct gaccatcctc gtcggcctga aactgtcgcg ctggatggcc
     1201 ggccagatcg tcggtcccgt cgaaaggctg cgcaatatgg ccgatgccat cagccagggc
     1261 ga
//
LOCUS       DAWCYS010000440         1236 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_5749_length_1236_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000440 DAWCYS010000000
VERSION     DAWCYS010000440.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5749_length_1236_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          353 c          359 g          272 t
ORIGIN      
        1 aagcggcagc gacgcctttg ccgccagcct gaccgacacg acccgcggcc gcgtcacctg
       61 ccggacaacc ggcaccatcg tcaagcccgt ggttagtggt taggggttag gggctagctt
      121 ccttttactc tataagtgta gtacagagag tttgtagttc gcagagagga ctttaaattc
      181 aaaaccatct gctaaccact agcccctaaa aaaatggctt gtcgcttagt gacaagccat
      241 tttctcatgc tttatacccg gtcatgaggt cgacgatgtt ttccagaggg tcgccggccc
      301 ggtagcgttt gagattatct gcggcgatgc ggacgatgta gtcgtgcgtc gcccggagat
      361 gatagccgcc ggagatgtgc ggcgtaatgt agatgcccgg cgtcttccac aagggggccg
      421 acgacggcag cggttccggg tcggtaacat ccagggcagc gccggccaga tggccctggg
      481 cgacggcttc ctgcaaagcg gcctggtcga cattggtgcc gcggccgaca ttgacgaaat
      541 aagcgccttt cttcatggcc gcaaagcgcg cggcgtcata gatatgatac gtttccggcg
      601 tatcgggcag gcacgaagcg acgatctccg ctttcgccag gtattcattg aaatcggcca
      661 tatcccccat ttcgtcggct tccggcggta cggcaccctg acggcggcgc aagccgatga
      721 cgtgagcccc catggccttg acgcgctggc cgaagttgcg gccgatattg ccaaaaccga
      781 cgacgacgac tgtcgcgccg tagagggatg tgaccggccc ttcgtcgtgc cacagggcct
      841 gatgctgatt gtcatagtaa agatagagtt tcttcatcat agccagtgtc tgggccagca
      901 tgtgttccga caaggccagg ccataggcac cggtcgcatt ggtcaaggtg acgccggccg
      961 gcaggatacc gggctggcag tattcgttgg caccggcact gttgagctgg agccattgca
     1021 gctgcgaggc cttcttcacc aggtcaggag cgatattgcc aatgatgacg tccgcttcat
     1081 ccagcgcctt gccggcctgg gtcccatcac cgggacaata gcaaaaatcc gcttccggag
     1141 cagctgtttc cagccactgt ttctgttttg catcgacatc catgacaact aaaatcttca
     1201 aacgaatcac tccctattgc tttttttgtt tagcgg
//
LOCUS       DAWCYS010000441         1235 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_5530_length_1235_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000441 DAWCYS010000000
VERSION     DAWCYS010000441.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5530_length_1235_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          295 c          317 g          285 t
ORIGIN      
        1 catctcgcct ttgaggacgt tgaaccgctg cggcaattcg tctaagctgg tcttgcgcag
       61 gaaggccccg ctcttggtga tgcgcgggtc gtttttcaat ttaaattctg tttcccattc
      121 tttccgggcc tgcgggtccc gtaccaagag ttctttcagt ttgacgtcgg catcgacgca
      181 catgctgcga aatttatagc agttaaactc tttgccatcc cttccgacgc ggcggtgttt
      241 gaagataatc ggtcccggcg aatctttgta aatccacagg gcaatggcca aaaagaacgg
      301 cgacaagcag ataatcccca gactggtaca gatgacatcg aagacgcgtt tgacagcccg
      361 attgtaccag agagccaggt tgttgcggaa gctgaaagaa acgatatggc cgtcgatgag
      421 gctttccgtg tcgagcgtcg ccaacggcag gctgcccata tcggggataa aagcgacttt
      481 cttgaccaag ggctggacgc gatagacgat gttctggaca gcgttactgc tgaggcccgg
      541 tgcaatgacc atgacgtgct ggacaccggt ctggcggata acatcttcgg catcttcaaa
      601 acggcctaaa tgcggcatca tggccgctac tttcgcttct ggcgtatagt cttccagata
      661 gccgatgaaa tcatagctga cgcccgtatc gtgcttaaaa taatccaata cgatggctgc
      721 tgtcttaccg gctcccatga tgaggaccgg cagctggagc agtttgaaat gccccaggat
      781 atgtttgacg atgtatcgtt cggctaccag gaaaaagaaa gaggtcagcc acaataaggc
      841 cacaaagagt cgcgacgtcg aggctgccgt ctgggcgata tagataaaaa agatcagtaa
      901 agcgacggca taaaagacgg atttaaaaag gccggaaatg acctgccaga actgcatacg
      961 tcgcgtataa agatgatgta gttgcaaaaa aaggatatat acgaccggta tgatgacgtg
     1021 gatactgagc caggagagat ggaaatgcgt ttcgggaacg agaaaatccc gcagtccata
     1081 agatgcatac tcggccagcc agatagcaat ataatcagca ataaccataa caatcggcaa
     1141 caattcattg ctgtatttat ttttgataga aatagaccca ttcgtcgata gcgtctccaa
     1201 aatagctcct cctctgattc tcccctgata ataag
//
LOCUS       DAWCYS010000442         1230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_9126_length_1230_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000442 DAWCYS010000000
VERSION     DAWCYS010000442.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9126_length_1230_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          304 a          313 c          336 g          277 t
ORIGIN      
        1 cttcacgatc cgtgccaagg ccttcaagct gtcggccatg atccacggcc tggaagggca
       61 ggaacgggcc atgaaggaaa tcgccgccaa gggcaagagt gcgtcgaaaa tcgaacagga
      121 cctctattcg gccctggaac tggccaagga aatgagcctc cgcggctttt cctttatgaa
      181 tgtcgacatc aaccgctcgg cagcgacgaa gttcaccatc tgcgacggca agatcctgcc
      241 gccctttacg gccatcgacg gcctgggcgc caacgtagcc gaacagatcg tatcggcccg
      301 cgaacaggcg ccgttcagct ccaaggccga cctcaagatg cgcggcaaag tgtcccagtc
      361 cctggtcgac gtcatgagcc aggaaggctg tctcggcgat ttgcccgaag atgaacagat
      421 cgacctcttc gccatgtagg cggaaaaaac atttcctcga cagattgact ttcgtgccgt
      481 ataatgtttt tagtatttaa cgaactaaag gaatctgtct tagaaagagg gattgtcatg
      541 gcagtgaatg aaaaattgcg agaccatttg cacgatcagt tatttcgtgc tattttggaa
      601 ttgaagagtg tcgaagaatg ttatgaattt tttgaagacc tttgcaccat tcaggaaatg
      661 aaggccattt cggcccgcct ggaagtggcc cgtatgctca aggccggcga tatctatgaa
      721 gacatcgtca agaagaccgg cgccagcacg gcgactatca gccgcatcaa gcgctgcctg
      781 gtctacggca gtggcggcta cgagaagatc ctctcccgca tggccgaaaa ggacccggat
      841 ttcctgaagt tatccaagta ggagggtagc cgatttcatg aaggctaaga ttgatgtcac
      901 cattttccat aatggtgata tggatatatt gcatgcttcc atttacgaag agttgtggaa
      961 ggattattgt acatttaaaa aacgggctgc catgcagcag gacaagggga ccaagaaggg
     1021 cacgttcctg gcccggcgct attaccgggc ggccctcttg tccctgttcg ctttctttga
     1081 aggcgtcttg aacagctggg tcaagaccgt catccaggaa cggcaggaat ttgccggcgt
     1141 cgaacggcag gatacgctca agaagtgcga cgccatggtg gaatactgct ttttctgctc
     1201 ctataccaag cggcccggta cgttttgttc
//
LOCUS       DAWCYS010000443         1206 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_1134_length_1206_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000443 DAWCYS010000000
VERSION     DAWCYS010000443.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134_length_1206_cov_7.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          287 a          379 c          303 g          237 t
ORIGIN      
        1 atatgcagct gacggcagat tatttcatgc actcctttgt cagcttcaac gacagttata
       61 atatcttttt ctatgacttc ccggctgacg atgccttgta tgtcaaaatt atcccgcgct
      121 ttctgaccaa ccccctctac gtcggctata tgattcccca aatcgccaac ggaggccatt
      181 tagcgcgctt cattcatgcc ttgcagcagc gcttgcatga agtataagaa aggacggtta
      241 ttacaactct atgcgtttat ttgcaatcgg tgatctacat ttatcaggca atccccccaa
      301 aaagcccatg gaagtcttcg gcagccgctg gaagaaccat tggcagcgca tcgccgccga
      361 ctggaagagc cgcgtcagtg acgacgacgt cgtcctcatc gccggcgaca cgtcttgggc
      421 catgcacctc aaggaggccc aggaagacct ggaccagata cgggccctgc cgggtaaaaa
      481 aatcataatc cgcggcaacc acgattactg gtgggaaagt gccggcaaac tgaaccggat
      541 ggacgccgag aataatatgc agtacctcta cggctcgaca gccgtcctgg gcgacaagga
      601 catcgccatc tgcggcaccc acggctgggt ctgcccgggc gacatccact accaggaaga
      661 ccgcgacgcc aagccctatc ggcgggaact tttgcgcgtc gaacggacct tgcaggaagc
      721 cgaggccctg cagtgcaagc acaccatcct cctgctccac tacccgccgg tctgcgatct
      781 gaccaagccc agcggcttta cggacctttt ggaaaaatat cacgtcccgc tctgcgtttt
      841 cggccatctc cacggcatgc agcctgggac catgttcccg cgccgctaca acgggacgct
      901 gctccagctc gtctcggcgg actaccggga ctgcaagctc ttagaaatca aattcgacgg
      961 acaggaggcc ctgcgatgag tcaaatagcc aaaaaacacc tcttagccgg catcatcttc
     1021 ggcgacgccg aaacgggtga atacatctac ctccctggcg gcgaagtcgg gaccgaccag
     1081 ccgctctgcg tctttgaaca cgacggtcaa aaagaagacg tcgacctgga ccgggccggc
     1141 tacctcgtcg accacctgac cctgaaaaaa tgcagccacc ccaccttagg caagcggtcg
     1201 ttctga
//
LOCUS       DAWCYS010000444         1199 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_12250_length_1199_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000444 DAWCYS010000000
VERSION     DAWCYS010000444.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12250_length_1199_cov_3.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          258 a          305 c          323 g          313 t
ORIGIN      
        1 cctacaacct gatccagtct atccgcctgc tcacggactg catggattcc ttccgcaagc
       61 actgcgtcgt cggcatcgaa ccgaaccgcg aacggattgc gaccaacctc tacaactcgc
      121 tcatcctggt cacgggcctg gtgcccctcg tcggctatga caaggcctgc accattgcca
      181 aacaggcagc caaagaaggg ctgaccctga aagagtcagc cctgaagacg ggatgggtca
      241 cggcagagca gttcgacaaa gccatggacc cggccaaatt ggcaggtttg aaataagcct
      301 tacttatcct tttcgatgtt atctttccag gagtcgtcgt cttgctgctg gacggcactg
      361 cgcgtataag cggcaatggc ggcgaagttc ttctggacgc ccgtcgatgt attgcggtac
      421 ttgacggcat ggtcggcatc gatacccagg ttctgggctt ccgaagcggc gtcgatattg
      481 gctccgagga aaatgaagtc ccagccctgg gcttttttat cggaaatcat tttctggatc
      541 gtcgccttgg aatattccgt gctggcattt tcctgtccgt ccgtgatgat gacgaagatg
      601 accttatgat ctttgtcaga aatacccggc gtgtcggaaa tcttggcaat cgtgttgcct
      661 acagcatcga ggagagccgt cgagccgccg acgcggtaat ccttggttgt catgtccgac
      721 aggctgttaa tgttgctgcg gtccgtcagg aagtcgactt tattgttgaa caggaccgtc
      781 gtgaccttgg tgtcgacgtc gaggtctttt tctttggcaa tcatggcgtt gtagccgccg
      841 atagtatcgc tttccaggcc atacatggag ccgctcttat cgaggaccag gaccaggtcg
      901 agatgtttga tcgtcgacgg cagggactgt gtttcagcct ggcgggcctt gtcggtcatg
      961 gtcgtcgtgt cggccgcgac agccgcgccg cagcagagca gggcgctgag ggtaaatatg
     1021 agtaattttt tgaacatggc agatgcctcc ttaaaaatgg acgaatgacg tttcttttat
     1081 tgtatcataa aagttgttac aaagaatacg cagtctgttc acaaatcttt atgattcctt
     1141 taacttttgc ctaatctttc gttagaaatg accgttatac tggccttatc aattaggac
//
LOCUS       DAWCYS010000445         1195 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_10860_length_1195_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000445 DAWCYS010000000
VERSION     DAWCYS010000445.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10860_length_1195_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          345 c          356 g          242 t
ORIGIN      
        1 gaatttccgg cgattttttt tgcgcctttt ggccagccag aagacgaagt cctgactgat
       61 gcccaaggga cagaggacgc ttcaataaat ccgtcccagg agcagggtca ggatggcgat
      121 gacagcgatg acccggagat tcccggccag gagggccggc aggaactgcc attgcggcag
      181 cacaggcgcc acgataccgc cgaaggtcag gaccatgacc cccatcagga gccaggctaa
      241 gacgatacgg cttcgttgca gcatgttccg catctatatt accccctttc ccgtatcgcg
      301 cccgaaatat tggacgacgt catccatggc ccgcacgtac ttagggatgt cgatatgctg
      361 cggacatttg gacaggcagc ggccgcaggc cgtacagcgg gaagcgcgct gcgaccgttc
      421 caggttattc tgataatggg ccaggaaggc tttttcctgc gctgccgagg ctccttgcgg
      481 atggtccagg tcgattccgg ccggttcgcc atagcggttg tagatatgga agacttcggc
      541 gatgtcgacg ccataaggac agggctcaca ataccggcaa tatgtacagg gaatggcccc
      601 gctgccggct tccttcccca gggagcgctg gatggcccgt tcttcggcag ccgacaaggg
      661 ctggaaatcg ctcagggtcc ggatattgtc caatacctgc tctaaattgc tcatacctga
      721 attcatggtg atgacccctt ctttagagcc gacccagcgc agggcccacg aggccatgct
      781 ggcctctggc cgctgctgct tcaagatggc ttccgccgac gacgacagat tggccagatt
      841 gccgcccttg accggttcca tgacaaaaat ggggacatcc ttttcccggc acttgcggta
      901 caggtccccg gcctgccggc tccccgacgg cgcttcgccc ggttcgttcc agtcggcata
      961 gttgagctga atcatgcaga aatcccaatc atactggtcc aggagataat ggaaaaaggg
     1021 cacgtcgcca tggaaggaaa agcccaggca gcggatgcgg ccccgggctt tttcctggcg
     1081 gaggtagtcc aggatgccgt cgtggatgta cagctcatcg aattggtccc ggctggtcag
     1141 ggaatggagc atgtagttat cgaaatacgc cacctgacaa cggtcgagct gggtc
//
LOCUS       DAWCYS010000446         1170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_499_length_1170_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000446 DAWCYS010000000
VERSION     DAWCYS010000446.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499_length_1170_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          305 a          298 c          315 g          252 t
ORIGIN      
        1 atcaacgacg caccggccct ggcctttgcc gacatcggcg tcagcctcgg cggcaacaag
       61 acggacatcg ccgctgaatc ggcagccatc accatccgtt cggaagaccc gtcgaaactg
      121 tacgatgccc tgtacatcgg cagggaaacg atgcgggcca tcaaccagaa cttcacggct
      181 accattgtag tcaactcggc ggccatgctg ctcggcgcct tagggaaaat cagccccctc
      241 tgggctgctg tcatccacaa cacagccacc ctggccgtcg tcctcaacag cgtccgcatc
      301 ctgaaaccgg cccgcccgac cagacgcccg gcatagggaa tcgcgcatgc caatggtttg
      361 tagggacccg cgcatgccaa tggcttgtag ggacctgcac acgccaatgg tttgtagggg
      421 ctcacacgtg gcgagcccgt ccgggagatg aatacattcc catatcaagc agaaacgggc
      481 gccccacgtg gcgcccctac aaaaggactt gtcgttatgc gacaagtcct ttttataaat
      541 gaatttcaat agcaactatt gacagggttt gtgaaaaaat atataatgtt tatggtaatg
      601 aaatgtatta tcattaatta gaagggatgt gtgagtatta tttacggagc tgaaaggagc
      661 gatgtaaatg agtgtcgtca tcgtaggtgg aaatgattgt atggtttgtc gctataagcg
      721 tatttgcaaa gagtacgatt ataaggcaaa agtctttaca caggtaccaa gcgatttcaa
      781 atcgaaaatc gggtcgcctg atcttgtagt gttgtttacc agtaccgtga gccacagtat
      841 ggtccgttgc gccatcaaag aagcgcagcg caagaacgtc gaagtcgtcc gttcccatac
      901 gagcagctcg aacgcgctga agaacatatt agccgcccgg atggaagagg ccgtctgagg
      961 caagcaaagg ggagttagtc atgaaaattg tttcattagc cgatatgaaa gtcggccagt
     1021 cgggcgttgt cgatgccttg aagggacggg gaaatatcca gcaccgtctc gtcgacatgg
     1081 gcgtcgtcaa gggctcgcat atttcagtct tcaaaaaggc cccgctcgga gatcccattg
     1141 aggtaaaagt caaagggtgc gcactggcac
//
LOCUS       DAWCYS010000447         1155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_11396_length_1155_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000447 DAWCYS010000000
VERSION     DAWCYS010000447.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11396_length_1155_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          306 a          270 c          277 g          302 t
ORIGIN      
        1 caccttgtct gttgccgtca cggtaccgct gtactcggcc ttgaccggcg tgtccgcggc
       61 gacgactttg tacgtgttga ctgcaacggg agccttagtc gcactttggt tggccccaca
      121 gccggctaag agcgcggcga tggtgagtac ggtaacgctc atcgtcgcaa gtttgcgttt
      181 ttgtatcatc ttcactttcc tcctatttct ttcgattcct gatttttaat gccataaagc
      241 aagacgttga tacagcgctt catatagtca ctaaaggtgg aatcgtgttt gacatcatta
      301 aaataatcga taatgtcatc ggctgcgcta tgcaggatac gcaagaacag gaacttatca
      361 aagggcttga attctcctgt ctcctgagct tcatcgagga gccgttccaa aaggagccag
      421 tcttttttat agcgggactg caagcgttca caagtaccgg gcatctttgc caggagatcc
      481 gatgttttcc gcacggacag gacctgccag ttgtcactgg gcacgtcaaa ataagcgatg
      541 agcttggctt cgcaagtcat ggccggatcg ctgacgatac gctgatgata ggcatagatc
      601 ttatcggtaa aatcggcgat ggcctgttcg atgatttcct gtttcgacga aaaattttcg
      661 tataaagtcc gcttgctgat gcgcagatta cgggctaagt cgtccatgtg gaaatcggaa
      721 ccatggcggt tgatttcacg aagggcttcg gccagaatct tggcgcggat attttctgtc
      781 atgtaaacac gtcctactca aaaaaataca aggtgatact tttgaaactc aaactataaa
      841 actcagtacc aatttatagt ttaatataca gcaccttata agtcaataaa caagttgata
      901 ttattatttt ctcaattctt cggcaaattt ccgaatcggc aggactttgt ataaaacagc
      961 accacaggcc gcaccaatac aggtattggg caaaaaggca acgatgaaga accataaggc
     1021 gacgactttg gaatcgagga cgaaagcggc gacaggatag gcgatgcggc cgcccagaac
     1081 gccggtcccg acgatttccc cgatgacggc accccagatg gaatttgttt ttttatacaa
     1141 aacgccggat aaagc
//
LOCUS       DAWCYS010000448         1151 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_8881_length_1151_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000448 DAWCYS010000000
VERSION     DAWCYS010000448.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8881_length_1151_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          355 a          297 c          269 g          230 t
ORIGIN      
        1 aaagacgccg ctttcccgca cgccgccgat gacgaccatg agacagaaca aaatcgccag
       61 tacaggcata ttgatatacc cgacataaga tgacgaaggc ggtacggcca gcatcgacac
      121 cgatgccgcc actaccgcca ggacaagtac cagattctct ttgagtactg aaacgacaga
      181 tttcatgcta tgacctcaat cgagctgctt cccattttca ctgcggacct gggccgacac
      241 gaggagcgtg aagacgccgg taccgccaat gatattgccc aagcctgtcg gcaggaggaa
      301 gcggaagaag taatcgtaca aatcagagcc acccatgagg acatcataag cggcttcaca
      361 agagccgacg acgacgtggg caaagttacc gatagaaatg aaataggtaa aggtcaaaat
      421 ggtaaagaaa gcgaagctcc gcgacatagg catgatccag acgatagccg caatgagcat
      481 acctgccggg ataccgcgca taatgttatc catcgcgtca ggcgccatga tgtgatgcga
      541 cacggtcagg aggcactgcg taatttccgg cgtcgtaaaa accgggctgg acatgaaaag
      601 cgctgcaatg aacgtaccga cgatattggc gccgaagaca atgccccaca agcgcaggag
      661 tttccgaaaa ttttcccagc aagggacacg gaagacagga ataaccgttg tgatcgtatt
      721 ttctgtaaaa agctgcaagc gcccgagaat gacaatcaag aaccccgtcg tatagccgaa
      781 gcccgtaatc gctcccgtcc aaggggcctg cccgacatag acatggataa tggagcggaa
      841 ataaaacgaa aaagaaatca tcaaacctgc cgccaacgcc gacaaaatca aaggccatgc
      901 cggccgttcc aattccagtt cgccatcgtg acggatgatc tcaaaaataa gattgggcga
      961 taatttctca tgttcttcga cgatgacacg catcttctgg tcttctgact gatgacaaga
     1021 aaaatcacag tcatgattac accgtttaat catggatttc acttctttct gacatttttc
     1081 tttcattata ccatgtttct cttatcccac atataaaaac aaggccctgc accattgata
     1141 aggtaaaata t
//
LOCUS       DAWCYS010000449         1148 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_13837_length_1148_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000449 DAWCYS010000000
VERSION     DAWCYS010000449.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13837_length_1148_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          245 a          275 c          284 g          344 t
ORIGIN      
        1 cgtgccgccg aagagcgttc ctgggtctgt tgcgttatcg ctgatatagt agcttccgac
       61 gggatgcgcg gccaggatgg cgtctttctt tgcctgcgtg acggtgtcgg tgatgagctt
      121 cttaagggct gcaatgtcga tggcctggcc gttgatatcc gcggcggcgc ggacgcccgt
      181 gccggacgtc tgggagtcgt gaagcgcctc gaggttgttg ttgatgtaat ccaaaatacc
      241 ggtgttgtcg gcattgacca agggggacgt acgtccgtaa gtccccgcct ggatgatgtt
      301 attgctggcg tcccggagct ccgggacttg gtaagcctgt tttttcatca cttcgcctcc
      361 gtttccgtag cagccgtgga tgcggcctgc gtgtctgtgc cagtggtatc ggcggttgtc
      421 gtcgcatcga ctgtggtagt tgcatcggat ttaagcgtat cggccgacgt gtccatcttt
      481 gccgtggttg tagtgttacc cgctgtgacg gagccggtga cggggtccac ggtcgtcgtg
      541 gcttcgctgg tatctgcttt cgcttctccc ttcttggtga cagtcatgac gacagcatca
      601 ccataatcaa aatccgtatc agtatcggca tttaaattaa tcatgtttcc gacgtcatcg
      661 ataagcgtgt acgtcgtacg ggatgcagcc ccctgcggat atgtgacctt gccgtttact
      721 ttaaattctt ttttcatgtt gattccctcc tataaatttg atacgtcaac tcgactaaaa
      781 gcaggcgaaa aataataagt atctgttaaa tactttaaca tctattcctc ctttagttcg
      841 tatatagatt caaaggcttc tttcggcatt gtactttcta tatattcacc gttcgtttta
      901 tagcggaata catagtcacc agaatcattt tgatatgctt ctacttcaaa cggcttttga
      961 acatactttt tatattccat aattacctcc tacgatatag aatagggaac cgtattcggg
     1021 tcaatagcaa tgaaccctgc tgtcccctgg caaacagtaa catctgagtc taaattagta
     1081 cggtatgacg tcagtttccc atacgaaacg ctataacact ctccgtaaaa ggaaaatgta
     1141 accgttac
//
LOCUS       DAWCYS010000450         1147 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_3784_length_1147_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000450 DAWCYS010000000
VERSION     DAWCYS010000450.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784_length_1147_cov_8.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          291 a          310 c          284 g          262 t
ORIGIN      
        1 gaacgccagc gtgtcgccag gacggccgat gacccgcttg acccagatgt tcttggtctg
       61 cagatcatga ttaaagaaag ccatgtaatt gttcatcggt tctgcgacgt catctttcca
      121 cgtgcgcgga tactggacgc ggctgtcgat gatgacaata tctccatagt tcgggacttc
      181 ccccaggacg tgattccatt tggatacgat gaggtattcc ccgttgtgca gggtcggcat
      241 catcgattca ccggaaacgc gtgtcggctg gaagaagaag atatggatga ccatggcaat
      301 ggccaacgcg atgatgatgg aataaatcca ttcgtaaact tcatgtagaa acgtattcac
      361 gctgaaacct cctcacttgc gtactcgtat attgtagcaa accaggggta taaaagcaag
      421 ggtcagagca ggtcgctttc ccctatgatt tcaaggcctt ctgcgcgtaa taacgccgcc
      481 gtcacgccgt cgccagccgt cagggtccgt gaaaaagtac catcgtaaat ctgtccatgt
      541 ccacaagacg gacttttctc tttcaagatg gccttcgtgc aatggaacag gcgggccaag
      601 tacagggccg cttcggcacc acgccggtat tgcgccgtca catcggtacc gtctgccgtg
      661 acgacccgtt ctccctgccg ttctgccggc ggacgcggtg tcgccaggcc gccggcctgt
      721 tccggacaga cgggaatcaa atggatatca tcccgcccga gcaaatcctg cacctgcgga
      781 taaaccttcg tctgaccatc atagcggcag tactggccga gcaggcaggc actgattaga
      841 acgttcataa aatcctccct ctcaaatgat tgaaaaacaa gctataccat attataatac
      901 aggttgcaac acgaatttca acctatccat ctatcgtcaa aggagtgttc acaccatgga
      961 acaaaagaag acgcgcaaca cagctgactt tctcgtcgaa tgtctggaag aagaaggcgt
     1021 ccattatatt tttggtattc ccggcgaaga aaacctcgcc gtcatggatg ccctggaaca
     1081 ttcaaagatt gaattcatta cagtccgcca tgaacagggc gcagccttca tggccgatgt
     1141 ctatggc
//
LOCUS       DAWCYS010000451         1144 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_2914_length_1144_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000451 DAWCYS010000000
VERSION     DAWCYS010000451.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914_length_1144_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          221 a          311 c          339 g          273 t
ORIGIN      
        1 ccttatcctg gccgatgagg cgggctgtcc gctgggtata ttctttcttc atcgcctagc
       61 gctccggaat gatttcccgc cagtagccgt cggggtcgac gatgaaataa atgcccatgg
      121 ccgggttctg atagacgacg cagcccatgt ctttgtgctt ggccagggcc gcatccatat
      181 ccttgacggc aaaggccgtg tggaattcgt tttcgccgag gttatacggt tccttgcggt
      241 ctttgagaca ggtcagttcc agctggaagt cggacacgcc gtcgccgaga tagacgatgg
      301 tgaaatccgg gttctcgatg cggctgactt cggtcaggcc cagggcttct ttgtagaagt
      361 ccatgctgcg ctggagattc aaaacgttga aattggtgtg cttaaaggtg aattgcatag
      421 tactaccccc tttttattta ttggttctct tcctgccagc ccttggatac gtcgacccat
      481 tccctggcat gggaataggt ttccagttcg ggcagtgtca gttcgatggc actgttggcg
      541 gtgccgcagg ccggaaagac ggtctggaag cgcttgagcg attcgtccag gaagacgtcg
      601 acgccttcgt tgatggcgaa aggacagacg ccgccgacgg gatggccgat gagccgttcc
      661 acgtcaccgg ccttgatcat ggtcgccttc ttgtggaacc gttctttgaa cttatggttg
      721 tccacgcggg catcaccggc aaaaaggacc aggatcggat ggtcgccgat gaggaaagac
      781 atggacttgg caatgcgccg gccctccgta tgaagggcgg ccgccgcttc ttcgacagtc
      841 gcactggact ggtcgagttc catgacccgg tctgccatcc cccaccgggc aaaatagtcg
      901 cgtacttttt gaatagacac gttgctcccc ctctcaattc gttataatcg tattatatac
      961 taatcacggc gctcaggcaa actcaaagga cgacgtactg ccccggtcgg tcttgtggac
     1021 ggccaggcgg gcgtcgatgc gttccttcag ttccggtacg tgggagatga tgccgaccag
     1081 gcggccgccg ctgcgcaggg acgccagggt cttcagggca taatccaggg tttcaccgtc
     1141 aagg
//
LOCUS       DAWCYS010000452         1135 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_2868_length_1135_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000452 DAWCYS010000000
VERSION     DAWCYS010000452.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868_length_1135_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          236 a          283 c          327 g          289 t
ORIGIN      
        1 agccgatggc atcggcatgt tggacgacga agtccaggtc tgctttgtcc ttttccgtca
       61 aaggcgacag gttcatcggc gtctgcggga agttcaggct cttctggttc ttaattttcc
      121 cgcctttcgg cttggtgtag gtaatcttca ggtaggcccc cttttcggtc aggctttcga
      181 cgacggcccg gattttccca tcgtcgatga ggacgggctg gccgctctga aggttttcaa
      241 agacttccgg gatgctgcac gacaagacga tatttcccgt ataatctgaa ggatacgatg
      301 aaatggcgcc ggatacgagg aagacttgtt ctcccggcat gaccttggct tcgccatagc
      361 gcagcaggac tgaggcaatg cggactttcg gaccggccag gtccatgtag acattgcagt
      421 gatgccccgt ttcggcttcg tcctggcgga tataagaaat catggcatcc cagacttcgc
      481 gggaatcgtg ggcgcagttg atgcgggcgc agtccatgcc ggcggcgacg aggttgcgga
      541 tcagggtccc gccatcttcg gcggcttctg tcggcagggt caccatgata cgcgtccccg
      601 tcttggccgt accaaaaatc cgccctgtgt tataggccag gagcgtatca ccgtagaaga
      661 agcgctttgt ctggggataa gggatgagtt tatctgccgg ttcatggcaa atccggccga
      721 gggaggccat gacggcatcc agattggtaa tcgcccgagc ttcgccgcgt cccagggagg
      781 acaggcccaa gggcaggagg gagcgctgga ttttccgcag gtcatggcag cgcagagcta
      841 agtaaaaggc catattccag gcgctataaa cgaagcgtgt ccgctgtatc tgcggcagcc
      901 agtgctggaa taaggcgtcg ccccgggaaa ggacgtcgcg ccggagctgg cagaggacgt
      961 cgtagagttc gttcgtcgat gtatatattt cctgttccaa tgtaatcacc tcaatatcat
     1021 gaattttctt catatctatt attatattcc cgacatgtgg ctttctcaat gctttctgtt
     1081 ttgttttaca gcgattttat agatatattt tttacatatg gataatctat gatat
//
LOCUS       DAWCYS010000453         1132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4653_length_1132_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000453 DAWCYS010000000
VERSION     DAWCYS010000453.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4653_length_1132_cov_9.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          303 c          307 g          270 t
ORIGIN      
        1 agttgtggag cgtttccata ccgccaatgc ccgtaccgat gatggtgccg atgcggtcgc
       61 ggtcttcttc atccagattg attttggaat cgtcaagagc cagtttggca gcagctacgg
      121 agaactgcgt ggacgggtcc atatggcggg cttctttacg gtcgatgccg tagtcagcgg
      181 ggttaaaatc tttgacttcg ccggcaatgc gggcagcata ttcggttgcg tcaaagtggg
      241 taatggggcc gatgccgttt tcgccgttga gcagggcatt ccagaaagca tctttaccga
      301 tgccaacagg agaaatgaca cctaaccctg taattactac gcgtttttcc aaagttttca
      361 cctcattata aaaagtaacg tactatcagt cgggaatggc ttccatttcc cgtttgatgt
      421 tggcaaatat atccttgacc ggcagaatgt cgtggatttc catcatgcgc ctgccggaaa
      481 ataccagacc tgtttcgaca tcgccctgct gagcccgcgt caatgcccgg atgatgcagt
      541 atttatgact gcacttcttg agacactggt cacatgtttc cggcttcggt gccgtcccca
      601 ggaggatccg ttcggagaaa ggcgtgcgga tagcctggcc gggaaggccg acaggactgt
      661 gtatgaggat gaagtcttcc ggtttattgg cccggagata gactttcttc aattcttcag
      721 aaccattcga ttcaacgctg gcagcaaagc ggctgcccat ctggacgcca ttggcgccga
      781 ggcggagcat ttccgcaatg tcttcaccat gcagtacccc gccggcaccg ataacaggga
      841 tgtcaacggc agcgcggact tccggcacga tgaggcggct cgaacggttg gtccccaaat
      901 gcccgcctgc ttcagctcct tcgacgacga cggccgaagc acccagggat tgggatattt
      961 tagctagttt ggcagaagac acaataggga caataggcgt gcccgatgct ttgccccagg
     1021 cgaacatgtc ccgggaaaag cctgcgcctg ccacaaccag atcgattcct tcttcaatag
     1081 cagtggtcac gatgcccttg aattcacggg ctgcaaccat tacgtttata cc
//
LOCUS       DAWCYS010000454         1132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4870_length_1132_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000454 DAWCYS010000000
VERSION     DAWCYS010000454.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4870_length_1132_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          249 a          345 c          317 g          221 t
ORIGIN      
        1 accagacact gcgcatcgac gacccggtcg gggctattgc cgctcacggc ggctgcggtg
       61 ccttggggac cctcctgacg ggcctgttca gcgtccaaga cggcctgttc tatacgggag
      121 aaatccgtct gttcctggtc cagctggccg gcgtcgtggc tatggccctg ttcgtcttcg
      181 tcgctatgac cctggttttc cgcatcctcc aggcgacgat aggcctgcgc gtctcggcag
      241 ccgaagaaat caacggcctg gacttcaagg aaaacggcct ggtaccggct tacgaactgg
      301 accgcagcga cgatttggag gatttgtcgc aagccttgaa ggccggggaa gcgccggaag
      361 gggcccaaga aatccccttg gaccaggcta tccccgattc ggtcatggcc agtccccagc
      421 cggtcggcgg ccatgccctg tataacgtga ctatcattac cgatgaaaag cgctttgaaa
      481 ccttgaaaga cgccatggaa gccatcggca tcacgggcat gaccatcacc cgcgccctgg
      541 gattcggcat cgaaaaggga cggactgaat tctaccgcgg ggccaaagtg tcggccaagc
      601 tcctgcccaa ggtccgcgtc gaaatggtcg tttccaaagt gtcgccgcgg accatcatcg
      661 aaacggcgaa gaaggtgctc tatacgggca attacggcga cggcaaggtc ttcgtgtcgg
      721 ccatcgacaa cgccgtcaag atccggaccg gcgaagaagg ctgcgatgcc ctgcaggact
      781 atcccgtcgt ccagaataaa tgacctttta cagacatttc ccttgatttt tgcaaaaaaa
      841 atacaggctc tggctatact gaaactgcac agccagagcc tgttttttca tggatgaagg
      901 aggaaatgcc ataagaccat atcctcatcc ggacctatga ccatacccgc ggcgagtggc
      961 taccggcctt cgaccgtacc gtaaccctgc cggaatggtg ctgcctcacg gcgtgagggg
     1021 cggtgtggga acatcgcgcc aataccgacg ggtcgcccac gtgtcgaccc ctgcgattcc
     1081 ctgttgggaa aatcttgcta acccctagcc cctagccact aaccactaac ca
//
LOCUS       DAWCYS010000455         1119 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_9212_length_1119_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000455 DAWCYS010000000
VERSION     DAWCYS010000455.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9212_length_1119_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          267 a          337 c          310 g          205 t
ORIGIN      
        1 aagcaggacc gcacgccggt cctcctgcac gaacggctgc cggccgtcag catttccatg
       61 gaagacctgg atgccctgct gtaccagctc ctcaagcggc gcagcctgac caacaacgaa
      121 gcggtcatcc tacacttgac ttttgaatcc ctctatcgcc gcatcgacga tgaagggacg
      181 cgcctgctgg tcatcagcga cattttgaag aacctcaatc tccattgagt gatagaaagg
      241 acgtgagggc tatgttatgt gatgcctgta agaaaagaga ggctgttgtc catatcgtca
      301 agatcgagaa cgggcggcgc acggatttgc acctgtgtgc agcctgtgcc aagaaacaga
      361 ccaatttttc taatttcaga gatttgaata tcattgataa cgactttttc cgcaagatgg
      421 cctacccgga ctaccaggga cgcagcggcg acgaaccgcg ctgtgccagc tgcggtatga
      481 cctacagcga attcaaccgc ctcgggaagt tcggctgccc cgactgctac gaagccttca
      541 aggaagaaat tccgtcgctc ctgcgccgca tccacggcca cagcaagcac gtcggcaagg
      601 tgcccaaccg cggtaccggc gtcttccgga cggcgacaca cgtcaaacgc ctgcaccagc
      661 acctgcagaa actggtccag gaagaacgct atgaagaagc ggcgaaagtc cgcgatgaga
      721 tccgcgccct ccagcgtcag ctgccggatg gcggaaaggg ggaataagcc atggtccacg
      781 atatgctcca gcagccgctc tgcccctggc aggacggcac gggaaacttc aacgatatcg
      841 tcatcgacag ccgcgtccgc ctggaccgga acctgaagaa atacgtcttt cccgataagg
      901 cgtcggatac ggaactggcc gccgtcctcg aagagggcaa acgccagatt ggtacccgcg
      961 ataccttggg ccatgggcgc tataccttca tcggcctcga cgaattgtcg ccgctggaac
     1021 gggaaatgct ggcagtgaaa catttttcct cggccagtca tatcgaacag cccaagcacc
     1081 ggggcctcct gctccgtgaa gacggggccg tgtcgatca
//
LOCUS       DAWCYS010000456         1117 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_13284_length_1117_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000456 DAWCYS010000000
VERSION     DAWCYS010000456.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13284_length_1117_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          214 a          297 c          316 g          290 t
ORIGIN      
        1 cctgtgagtt cagcgacgtt gccgcctttg acgctgccgc cgtagaggat ggaaacgtct
       61 tctgccgtag ctgcgtcata gagggcagcc aggttcttgc gaatgatagc gcagacttct
      121 tctgcctggg cagcgctggc cgtcttgcac gtgcctctag cccagatcgg ttcataagcg
      181 atgatgactt tcttgacgtc tgctgccgtc aggccttcca aggctttttc aatctggctt
      241 tcgatccagg actgcatgat gccctgttcg cgctgttcca gcgtttcgcc gcagcaaaca
      301 atcggcgtca gattatgagc cagtgcggct ttggtcttct tgttgaccgt ttcgtccgtt
      361 tcattgaagt acgtccggcg ttcggaatgg cccaggatga catgggtgac accgatgtcg
      421 acgagcatag ccggcgaaac ttcgccagtg aaagcgcctt tttcttccca gtgcatgttc
      481 tgggctgcga cgtggatcgg cgtacctttg gtcatttcga cagccatggg caggtacggg
      541 aagaccggag cgatgatgac ttccggtttt acgtcctgcg ggactttggc gacgaggccc
      601 tggatagttt ctttggcgtc ggcaccgaga tggttcattt tccagttgcc ggcaatgatg
      661 gttttacgca taatgagttt cctcctttaa aggtgaatga ttttatttat cgctcaggga
      721 tacgacaccc gggagctgtt tgccttcgag gtattccagg gaagcgccgc cgcccgtgga
      781 gatgtggctg atcttgtcgg ccaggccggt cttgttgaca gccgagacgg agtcaccgcc
      841 gccgacgata gaaacagcat cggaatcagc aacagctttg gcgactgctt ccgtaccttt
      901 agcatagttt tccatttcaa atacgcccat aggaccattc caaacgatga gtttcatcgg
      961 agccagttct ttggcgaaga gttcctgtgt cttcgtgccg acgtcgagga tcatccaatc
     1021 agccgggacc gcatcgacgg gaacgacttt gtgatcagca tcgttcttga attcagcggc
     1081 gaccatgaca tcgaccggca ggagcagttt cgtaccc
//
LOCUS       DAWCYS010000457         1080 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_11211_length_1080_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000457 DAWCYS010000000
VERSION     DAWCYS010000457.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11211_length_1080_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          196 a          280 c          334 g          270 t
ORIGIN      
        1 cctttatcct ggaagatgtt cttcaggcgc agcgtgaagt agtcgtcgag ctgacccatg
       61 actttggctg cgtcttcccc ttctacgccg aggagattga ggacgaaggc aaagacttca
      121 tggcagttga ggttccagcc ggcatccatg aggatgttga gggtaccgat ggtctggcgg
      181 cgcagggcaa aggggtcctg cgagcccgtc gggatgaagc cgcggctgaa catgcccgta
      241 atggtatcga acttgtcggc aatgcccagg actttaccca tgtcggtctg cggcaggacg
      301 tcgccggcaa agcgcggctg gtactgttcg ttgatggctt cggccacgcc agcatcttcg
      361 ccgtcgatga gggcatattc cttgcccatg acgccctgga gttccgtgaa ttccatgacc
      421 atctgcgtag ccaggtcggc tttggccagg agcgatgcgc gctgcagctt ggcgtcatcc
      481 aggccgaggt cgagtttatg attgaagaag accgtaatct tttccaggcg ctgggctttg
      541 tcatagagcg tgcccaagcc gtcctggaag acgattttct tcaatttttc cgtgtaatcg
      601 gcgagcttat gtttcttgtc ttcttcaaag aagaacttgg catcgtccag gcgggcgcgg
      661 aggacgcgtt cgttgccgtg ctggaccgtt tccagatggt agtcgttgcc gttgcggaca
      721 gtcaggaaga ggttcatgag cttgccgtct ttatcgcgca tagggaaata gcgctggtgg
      781 tccttcatcg gcgtgatgat ggccgcttcc ggcagtttca ggtagtcttc atcgaattcg
      841 ccgcaaaggg ccgtcgggta ttcgacgagg tagacgactt cttccaggag gtcgtcgtcc
      901 atgatgatgg tgccgccctt ttcgtcagcc agtttctgca gaccttcgag gatcatctgc
      961 ttgcggactt ccgggtcaac gatgaggaaa tgttccttgc acgtttcttc ataggcttcc
     1021 gggctttcga tggtgaaatc gacggtaccc aggaagcggt ggccgcggct gacgttgccc
//
LOCUS       DAWCYS010000458         1080 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_3644_length_1080_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000458 DAWCYS010000000
VERSION     DAWCYS010000458.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3644_length_1080_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          242 a          312 c          290 g          236 t
ORIGIN      
        1 atagggaaca gcggccagaa gctgataagg tatctatcgc cgtcgtccag gtgcctaaga
       61 tttccaactt caccgatttc gacagcctgg cccgcgaaga cgacgtcgcc ctgtactatg
      121 cccgccatcc ccgcgacctg gccggcgccg atgccgtcat cctgccgggc agcaagaata
      181 cgacagaaga cttgtgtttc ctcctggaac aaggctggga ctgggcttta cagcatttca
      241 tcagccaagg gaaacccgtc atcggcatct gcggcggcta tcagatgatg ggccaggctg
      301 tctgcgaccc ggatcagacc gagtcccaac tgggccgcgt cgaaggcctg cacctcctgg
      361 gtgtgacgac gaccttcgta tcgcataaat tgacgtcgca agtgacggct gactgccgcg
      421 acctgccttt tgccggaacc atcctgcaag cgaagggcct gacgggctat gaaatccaca
      481 tgggccggac cgagcgggat ggcagcgata cgtatccctt tgtcgtcacc agccgttctg
      541 gagaaccctg tgaaagtctg gccggtgccg cccgtaaaga tggcctggcc tggggaactt
      601 atatccacgg catctttgat aacgacgcgt tccgccgcca attcctcaat gccctgcgga
      661 tccgcagggg atggcagccc ctgccggtga cgctgcacta ccggcaggaa aaagaagcga
      721 aatacgactg cctggcccgg atcgtccggg agtctctgga catgaagcgc ctgcggcaga
      781 ttatggaaga aggaataaaa tgattactgt actcaccgct tttatcgtag atttcatcat
      841 tggcgatccc catacgaaac tgcatcccgt cgccctcatc gggcgctgga tcagtctgct
      901 cgaacggctg ttctataatg agaaatcgac gaaatgggaa catttcatct taggcgggct
      961 gactgtcgtc attaccctgt tgtttgtcta caacataacc ctggcactgg tcctggactt
     1021 ggaattttta cctaacgaac attgtagtta tataatccag ggcctactct tatacttcat
//
LOCUS       DAWCYS010000459         1054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_8131_length_1054_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000459 DAWCYS010000000
VERSION     DAWCYS010000459.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8131_length_1054_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          274 c          276 g          208 t
ORIGIN      
        1 atgagcgcct gcgtctacct cgatgatttc acgcaggccg ccgactatct ggaccagttg
       61 agggcatact gcgagaagca gcattgcacg ccgaccggcg acgtcacctg ccagatcatc
      121 ggcgtcatcg acaaaaagga cttccgcaag acctccgaga tcctgcgcct ccaagtcccc
      181 gtcaaagtag gcgaaaaccc tcatcattga tgagccctgc ccagaaaatc gcatagaatc
      241 atcattcgac agcatacgac atgccataaa cgcacgttgt atgctgtttt tttgcaataa
      301 gagcatgtag aaaccgtccg ttataaagcg aaatgatttt aaatgatatc ttataacgat
      361 gactgtaaaa caaaaaacga aattgactct accactacca tagaccacat aattaggata
      421 tatttagaac gaaatgatag gaaatgataa gaaatggtac tttgaggagg atgtacaatg
      481 aatcgaatct ataaggtcat ctacagcaaa gcacggcagt gttacatcgt tgtgtccgaa
      541 ctggcaaaaa gcaaccataa aagcagccag cagggtgccg atcacactaa caccccagcc
      601 ctagcccgca tcatcgccgt agcccttgcg gccggtgccc tgacctgggg cagcgtgccg
      661 gaagtcagct gggcggcaga taccggacag gcagataccg gacagaacgc ggcaatcatt
      721 cagggcggca tccattattt cagtgtcaac cctgaaggtg ggaaccgcaa caagaattat
      781 gagaatgacg gtgctgtcgg ggtcgattcc ctagccgtcg gtgccggtgc ccatgcagac
      841 gggcgcttgg gtgccacggc cttgggcaac gatgccgtag ctaatggcga acgggccctg
      901 gccgtaggca atggctcgaa ggctacggga caggatacca tcgccatcgg aactggctcc
      961 cagggagaag ctgacggtat caatgccatc gccattggta atcagagcca tgccaagggg
     1021 acgtatacga cgactgtagg cggtttttct gaag
//
LOCUS       DAWCYS010000460         1052 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_5473_length_1052_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000460 DAWCYS010000000
VERSION     DAWCYS010000460.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5473_length_1052_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          310 a          292 c          279 g          171 t
ORIGIN      
        1 cgaaggcgac cacatgacct tcaccatgcc gaacgggtcc catatcgtcg ttgacgtgaa
       61 gaatgaaatc ctcttgacgg acgaagaact ggcacaggcg aagaaagacc accatatcga
      121 cgataacggc aacgtcgtcg tcgaaggcta cgcacagctc catggtaaag acgcttatat
      181 ggccctctcg cagggtagcc gtgaaaacac gggattccat gaagtctacc acctagcaga
      241 aggcgccgtc ttgacggacc gtgaaaaggc agctatcaag aaggccatcc cggacgccga
      301 aaaacgcgcc gacaagtatg ccgaatgggt agaagcccgc aagcacggcc gcggcacggc
      361 atggggcaag ctgttccaga aaatcaagga ctttgccgcg aagatgaaga gaatccttac
      421 caggactgaa accgtgaacg acatattccg gcagattgag tcgggcaagg tatgggaacg
      481 aggcgcccgc gataataatg aacggcgcta tgctgtccgc caggctgacc agaaagaagc
      541 gccggtcaaa ccgcaagaca ttatcgacgc tatcaacgat attgtccata tctacgaagg
      601 aagccgcctc accgacaagg aacggaagga actgagggaa gccagccaat tcgacccgga
      661 tcagaagcag gccgtccgtc cacaggctac cgacctgtat gatcgtcacg cccatgcagg
      721 ctttaaccgt atggggtact tcaatatgag caactacggc cgtatcctcg ccctgcatct
      781 cgacaacgtc atgcagctga aaggcaacct agaattggca aataaggtcc tggaccggca
      841 ggataagaac gccgccgaaa acaagatgaa cggcgtcaac gaacgtctta ccccggcaca
      901 agcacggcag aacgctgtca tggactttgg ggccatgatg attcgcaacc cggaactggc
      961 ccgtgaaacc tatccgactt attccaagat tttcgacgaa ggcctggaac agcatcccga
     1021 cttaaaagaa aagctcgaca aagtcattca gc
//
LOCUS       DAWCYS010000461         1049 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_12101_length_1049_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000461 DAWCYS010000000
VERSION     DAWCYS010000461.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12101_length_1049_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          268 a          325 c          271 g          185 t
ORIGIN      
        1 cggctggctc cagtcgcgca tccacgatgc cgaaggccgt tatgccgcct tggtactcga
       61 cgttgccggt gaccaggaag accgcctgac ggcactgaaa caggccgctt atgacttcgg
      121 tgccagccag catctcaatg ccactacggc cccggaagcc ttccaccgcc tggacgacct
      181 gctcctggac gggatgccct gcgcccaggt caaccgcgtc cgcgaaagcg atgacagccg
      241 catcacctgg gaccggacta tggacctgca cagcgaattc tggcagggcc acggcgaccg
      301 ctactacgcc ctgcgccagg ccttggtcga cggtctgtta tcggctacgg attacgcctt
      361 caccagcccg gccgaaggcc agtatgaaat cgtcaagaaa tctgcctaaa cgaaagagag
      421 ggagccttat gtattgcacg gaagtcatgg ttaaacaaca taccaatatt tcacggatgc
      481 tgaaagtcat caaagaatcg tgttgcgcta ttctggaagg ggctgaaatc gaccagcagg
      541 aatttgccga aatcctcgat ttcatccgcc gttatgccga cggccaccat caccgccggg
      601 aagaactcgt ccttttccct gaaatggtcg accacctgga agaagtcgcc tccatcatca
      661 tcaagcacgg catgctcgtc gaacacgacc tcatccgtgc ccatgtccgg gccctcgacg
      721 aagccctcaa actctacgcc caggaaccga agacggaata taaactccag gtcctgaccg
      781 aagccatggc ctatgccaac cgcctgcagc agcacgtaga aaaagaaaac aacgtcgtct
      841 accccttagc cgaccgcggc ctgtccgatg atatcaaagc taaaatcgac cagactgtaa
      901 aagacatgga acaagaagaa ggcccggaat tcgtaaaagg ctacatggaa ctcctggacc
      961 gcctggaaaa gaaatataaa atcgcgtaag gccgcctatg ggccgcccgt aggccgcggg
     1021 ccgcatcgga ctcatggtgg tattacgac
//
LOCUS       DAWCYS010000462         1039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_2156_length_1039_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000462 DAWCYS010000000
VERSION     DAWCYS010000462.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156_length_1039_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          191 a          314 c          262 g          272 t
ORIGIN      
        1 atcttatcgg agatgtcgag cggcttgccg ccggccacgt ggctgttact gtagtcgatg
       61 gccgacaggc tgcatatgtc atcttccagg cgcttccgct cgtgctcctt cacccgcagc
      121 cgtgcatcca ggcggcggat ggtgttcaaa tactcttcgg cttcactcaa ttcctcatcc
      181 ctccttcacg ccgttattca cgccgtcctt cagcgctttc acgacgtact ggttcaaggt
      241 caggccgtcg tcgttggcgg cgcccactag tttctcacgc aggcccgacg gcagggctac
      301 ggacagggcg catttgagct ggtcctccag ttccggcgtc gcgacgcgtt ccttaccgaa
      361 cagtttttcg tacttatctt cgtcgaagcc gcagccgatg aagtgctgca ggtcttcaag
      421 gcggatcgta ccgccaccga cgcaggccga gatgccgcag atcttatcat aggctctttg
      481 gagccggacg acgccgaagc cgaattcgtc atgaagcgcc atcagcgtac aggtcatcca
      541 gaaatcgacg gaacggtcca gggtcgactt gataagcatc ttatcatgca aatccagcgc
      601 tttgcggttc accggtttat ttttcagttg caatctccgg ccgacgtcga cagacaagcc
      661 ggacatgggc ggtttaacgg ccgcccgctt catcttcctt aaaaggctca ttttcttcag
      721 ctccttctac actatgtaac agcatcgtga gatattccgc ggctttggcg acgtccttca
      781 cgggagtccc tttcttgggg tatcggtaca aatatttgat gatgttcccc aggtaatagg
      841 cgtcctctcc cgaaaggccg cgcgtcatga ttccgatgac ttccttacat tcgatgcctt
      901 tccacgtata gtggtccggc ttcttgatgt catgcagcat tttcgcgtcc atacttacct
      961 tccttccctg aaatcctgcc acagctggct cacctggatg ccgtggttgt gctgatattt
     1021 cgccgaccgg tattcgtca
//
LOCUS       DAWCYS010000463         1038 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_13183_length_1038_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000463 DAWCYS010000000
VERSION     DAWCYS010000463.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13183_length_1038_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          273 a          293 c          253 g          219 t
ORIGIN      
        1 ggcatcgacc ccctggccgt caacgacatc cagggcatca tcagccattt gaaacggcgc
       61 ggcatgggcg tcctcattac cgaccacaac gtccgggaaa cgctgagcat cgtcgacaaa
      121 gcctatatct tgagtgaagg gaagatcctc atcgaaggca gtccggaaat gattgccaat
      181 gacccggtag ccaagaaatt ctacttaggc gaagaattca ggatgtaaca gggagtatgg
      241 aatgcgaata ttagataaat atatactaaa agaatttatt ggcccctttt tattcggcgt
      301 ctgtgccttt acagcagtct tcgtcgggac gggtacgctc taccgtattg ccaacctcat
      361 caaccagtac ggggcttcgg cctgggctgc cttccgcgtc ttcgtcctgg ccatgccgtc
      421 gatcatcgtc gtcacctttc ccatgtccgt cctcttaggg tcgctcatgg cctttggccg
      481 cctttccagt tccagtgaaa tgatcgtcat gcgggctggc ggacagaact tcatccgcct
      541 ggccatgccg attttcattg ccgctttctt catttccatc ggggcgacgg ccttcaacga
      601 atacgtcgtg cccaaggcca acaacgccta taacacgatc atcaatgaag aaataaagca
      661 caatgtcgcg ccgacgacgc aggaccatat catcatcaag aacaccaagg gctccgatat
      721 atccagcctc atgtatgccc gtcagtacga cgccaacacg aagcagctca aggatattac
      781 cgtccaggaa tttgaaaatg atacgctcat gcgcgtcgaa caggctgacc gggccgactg
      841 gaacggccag aaatgggtca tgcacgaagg catgatctat gacgtatcgg ccaatgaaca
      901 gacgacgcgg tccctgaagt tccagaacca ggccctgccg attacgcaga agccgaataa
      961 gatcaatgca tcccagaaga aaccggatga actgaccatc cgcgaactgc gggaacagat
     1021 taaactcttg gaccagaa
//
LOCUS       DAWCYS010000464         1034 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_5008_length_1034_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000464 DAWCYS010000000
VERSION     DAWCYS010000464.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5008_length_1034_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          247 a          291 c          304 g          192 t
ORIGIN      
        1 cccgtcccga agccaggagc tcatcggcct tgacgggctg tccgttgagc cacagggacc
       61 cagcggcctt caaggtcacg acgtactggg ccgacggctg ggcctgggcg gccgaagccc
      121 gcggcagctg gacgccgagg ctgtgcaaat cgaccatgga caaggaggtc atcatgaaga
      181 agaccagcaa aaagaacatg atgtcgatca tggggatgat cataatcgac ggctgttcaa
      241 aatcgcgaat attacggagg cgcatggtca ctgccccgct ttcacaggca tcgtttccaa
      301 tgcggaaaaa cacatttcca tatccgtaat gatgcggtcc aggcgctggg tgaaataaga
      361 atggacggcc agggccagga tggcgacgca gaggccgaaa gccgtggcga tgagcgcttc
      421 gccgacgccg ccggtaatgg ccacggcctg gccggactgg acattgagga tgctgaaaga
      481 gcagatcatg ccgctgatcg tccccaagag gcccaggagc ggcgccatgg tgacgatgac
      541 gctcagatag tagagacggc ggcgcagttt ggccacgaag atgcccgact gcatttcgac
      601 gaaggccttg acgtcaggac ctgcctgatg ctgaaagaca tcctgcaaaa tgaggggcag
      661 gccgccgcgg ccgtccatgg ccaagcgctg ggcctggtcg tagcgccgca gcttcatctg
      721 cgtgtagaat tgttcggcaa aggctttccc ggcgtccatg tctccataga aaatcgcccg
      781 ttcgatgcca atgtagccga cgaagagaga acaggccagg agcaggtaca tgaccgaccc
      841 gcctgcctga aataactgaa aaccctgtaa aatcgcttcc aaaataccct cctgtccggc
      901 ctgctgacgt gtcgaatgaa ggtacaaaat caaaaggagc cgtcttgtag cgacagctcc
      961 cttacgtatc ttttttacaa tctccttcct atcgctcgca ggtctatcgg tgattatccg
     1021 tcaggcagtt cttc
//
LOCUS       DAWCYS010000465         1020 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_2231_length_1020_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000465 DAWCYS010000000
VERSION     DAWCYS010000465.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231_length_1020_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          194 a          309 c          309 g          208 t
ORIGIN      
        1 cgtataggct gcgccttcat tgtcggcagc actcgccaaa gaacctgtcg tcatgcccag
       61 ggccagacaa agggtcaagg ccagggcaga tatcattttt ttcgttttca cgatacatac
      121 acttccccat gttgttgttt gattttctgg cagagccggt cccacagggc ctgcggttcc
      181 ggatggccgt acgtcccgag ccggatgtgc ggcttggccc ggccgtggta atcgctgccc
      241 aaggtcgcca tcaggccata gttatcggca atgcggcggt aaaaagcact cgtcccttcg
      301 tcgtgatagc tgcaataggc ttcgatgcca tcgaggcccg tagcgatgag ctcttccgtc
      361 agcggccggt tctgcttcat attggcaccg ggatgggcca ggacggcgac accgccgtgg
      421 tcgtggatga tggcgacggc tttggcaaaa tccatgaagg tcatgggcac gaaagccggg
      481 cctccctggc cgcagatatc ccagccaaag ttgacgtatg gcgcatcgct ccgcgcccca
      541 cccggccggt acggtgccag gacgggacaa tccgcattgc gcgggtccgc cagggcgacg
      601 cgggcgatat ggacggacgc aacggcatcg ccgctgcaga gcttccagat agccggccgg
      661 gcgaggacga tgcccagggc ttcgacggcg tcgaggacct tttctgaaat cgcccggcgc
      721 tggccgtata catccttttc aatggccgca aaaacagggt cctcggcatg gatgccatag
      781 ccgagaaggt ggaaattctt ccctttatac tggcagctga tttcgatgcc cggcaccagg
      841 ttcaagccca gccgggccgc ttcctgccgc gcttccggga tagcccgggc cgaatcgtgg
      901 tcggtcacgg cgacggtcgt cacgccggca tcggcgcatt ggcgcatcaa gtcggccggc
      961 tcgaattggc catccatact atatttcgta tgcatatgca gatcaagcca tgtttccata
//
LOCUS       DAWCYS010000466         1015 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_4289_length_1015_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000466 DAWCYS010000000
VERSION     DAWCYS010000466.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4289_length_1015_cov_6.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          231 a          265 c          298 g          221 t
ORIGIN      
        1 aactataaag caaacgggac ctagcacggt ggaataagcc tttatgcgac acggccccta
       61 cggtaaaaca ttaatcatgt aattctttgt ccagttcttc cagccgttcg atacgttttt
      121 ctgtcgacgg atgggtactg aagaggttgg caatggcact gccggcaccg gcaaaggggt
      181 tgatgatgca catgtgagcc gtcgccttcg tcgcacctgg cagggcataa tggtgagcgt
      241 agttatcgat tttcgccaaa gctctggcca gggcgatggg gtcgtcgatc atgcggcccc
      301 cttcttcgtc ggccatatat tcccgcgtcc gggaaatgcc gagctggatg atagacgcag
      361 ccaggggcgc tacgatagcc gtcaccaaga gggctacagg attgccttcc ccgtcgtcgt
      421 cgcggccgcc gccgaagatg gcagcccact gggcgatgtt ggcaatcatg ccgatggcgc
      481 tggcaaagca ggccacgata gtgctgatga ggatatcccg atgcaggata tggctcattt
      541 catgggccag gacaccgcgg atttcatctt cgtcaagaag gtccaggatc ccagccgtcg
      601 cggcgacagc tgcatgggac gggctgcggc ccgtagcgaa ggcattgggt acttccgtcg
      661 ggatgatgta gaccttgggc atgggcaggt ccgcccgctg agccagggcg gcgacgatgc
      721 ggtacaggta gtggtcttct ccgacttcct gtgcccgata ggctttcagg accatagtat
      781 cactgaacca gtaggaaatg aagttcatcg ccgtagcgat gaggaacatg accatgacgc
      841 cgctgcggcc gccgatgagg ccgccgatgg ccatgaggat acccgccagg gccgtcatga
      901 gaaatacggt ttttacacga ttcataaaat caactcctat caagaagcta aaagtcaaaa
      961 ggtaaaattc gttacactct cattatatac ggtcttatgt tcgttgtcaa tatgg
//
LOCUS       DAWCYS010000467         1015 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_9316_length_1015_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000467 DAWCYS010000000
VERSION     DAWCYS010000467.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9316_length_1015_cov_4.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          240 a          212 c          241 g          322 t
ORIGIN      
        1 cgccattgat ataaattcag ctaaaaagtt ccgttctcaa cgtccagcag gaattccttc
       61 aagtgtgcaa aatacacggg ggcattgtcg atcatatggt gatggccacc gtttggagta
      121 gttgcgaaac gggcgtgcgg gatggttgct gccatgcgcc ttcctgtttc aagcggcatc
      181 gtttcgtgtt cgccgtaagt gatgagcgtc ggcacggaaa tgtttttgat cttgtttctg
      241 atgtcccaac catccaaagc accggtgatg acgaattcgt tgtcgccttg gaagtaattg
      301 tagacggaag ttgcgacggt gcttgtcaaa tggctgattg caggcggctg cttgcgatcg
      361 acgtagccgc agttcagttt gtcgatcagc ttttggtagc gttcgttgtc atagtcgccg
      421 gaattttcgc attctttcat gaagtcaaga tcttcttttg aatattcgtg ttcgcgaatc
      481 ttattgatgt tgacgatata ttcggcaatg ttgtcggtca tgctggaaat gatgatgccc
      541 ttgaggtgtt tcggatattt caaagcatat tcttgggtaa ttgctccgcc ccatgactga
      601 ccgatgagat aaaaatcgtc gatgccgagt ttttggcgaa cctcttcaat ttcatcgaca
      661 aaatagtcca tttttaaaaa tttatcaacg ttttcctgct tggtgaaatc cggctgatca
      721 gaataccatg agccgagctg gtcatacatt gtaacctgta cgtcgatgtc ttttaatttt
      781 tcagcaaaat tttcataata ttcgtggttg ccgcctggcc ctccgtgaag gcaaagtaag
      841 cggatttttc cgccgacgtt gtcagtgtgg gtccataaat ggtagccgtt gtccaaagta
      901 agaattgtcg ttccttcacg catgataaca cctgatttct aatgtgatac attaatttta
      961 acactctttc ttagaatcag cgtcaaattt tgcatgattg tcatcggtaa aaccg
//
LOCUS       DAWCYS010000468         1001 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_2509
            k141_3828_length_1001_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWCYS010000468 DAWCYS010000000
VERSION     DAWCYS010000468.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37995932
            Sequence Read Archive: SRR5898979
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3828_length_1001_cov_5.0000"
                     /isolate="gSpreadC_ID_2509"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="India"
                     /lat_lon="23.26 N 77.4 E"
                     /collection_date="2018-10-13"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          190 a          299 c          302 g          210 t
ORIGIN      
        1 atacagaaac ggtccatttg cgcagcggcg gtatttccat agaaaacagg tgattccatg
       61 ataaaacgat ttgaacatgg cggcaatatc tatcagcccg caccggatgg cggtccgtgg
      121 ctggacttca gtgccaatat caatcctctc ggactgccgg agagcatccg ccaggccctg
      181 gcggcccatc tcgacgccgt cgtccattat ccggatccgg ccgggacggc gctgcgccag
      241 gccttagaag gggcctacgg cgtaccaggc gggactatcc tgctgggcaa tggcgcagcc
      301 gaactttttt atctctatat gcatactttc cggcctcagc gggtcctgct gcccgtccct
      361 tccttcagtg aatacgaacg ggcggcacgg gctgttgggg ccgttatcgg ctatgagtac
      421 ctgaaagaag acgatgactt tgcctttccc tgggaagggc tgcgccgggc ctgcggccag
      481 gtggactgca tcgtcatcgg caatcccaac aatcctacgg gcaccctggc gacggccgat
      541 gaagtcctgg ccctggccga agtcggcgcc cgcaagggaa cagatatcct cgtcgatgaa
      601 tcgttcctcg atttccttcc ctctgacgaa gcctattccg tcctggacca ggccggggac
      661 cgggataacc tgttcgtcat ccggtcgatg acgaaattct acgctctgcc gggcctgcgc
      721 ctgggttttg gtgtcacgtc gccgttgcgg cgcggcctca tggaggggca taaagacggc
      781 tggaatgtca atgtcctggc tcagtatgcc ggcgtcgccg gactccggga ccgggcctat
      841 cagcaggctt cgcgccaatt ggtccagaag gagaaagact ggctctatga acagatgacg
      901 gccctgccgg gtcttcacgt ctatcggcct tgtgtcaatt tcatcttctg gcgccttacg
      961 ggagaaggga agcggacggc agacctggca gacgccttgc g
//
